LOCUS       DAJOSY010000001       135043 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJOSY010000001 DAJOSY010000000
VERSION     DAJOSY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 135043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 135043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..135043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.001"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            204..797
                     /locus_tag="OHN27_000001"
     CDS             204..797
                     /locus_tag="OHN27_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2284516.1"
                     /translation="MKKTLLAAALVMVSGSALAVDGGHIDFNGLVQSGTCKVGVVDAG
                     MHGITTDGVVTLDTANVTDNFAEVNATAVGLLPKEFMISVNCEPGKTEASLTMGSASY
                     ANTSGTLNNNMNITVNGIQPAENVNIAVHNMTNKSGAAEIKQVHMNNSAEKHLLTLDA
                     NGDGQYVFNASYVKAPNSQDVTSGHVTTNALYTITYN"
     gene            880..1605
                     /locus_tag="OHN27_000002"
     CDS             880..1605
                     /locus_tag="OHN27_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCQ2284517.1"
                     /translation="MFPIIKKTVSALFISTLLTSAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTSTPPVSRIESKRGQTVKVMFTGTT
                     QLPADRESVFWFNVLEVPPKPKDAESDKNLLQLAFRTRIKLFYRPSGLKGEPAEAPAK
                     LTWKLNNAQLQVNNPTPYYVSFNDVAIESGGKSYKVDSSMVAPFGQVSFAVTDIASSV
                     SSGKVIYKAINDYGGNIDGSTSL"
     gene            1646..4246
                     /locus_tag="OHN27_000003"
     CDS             1646..4246
                     /locus_tag="OHN27_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCQ2284518.1"
                     /translation="MYTLTHQKSRLPKTTLLAACCAFFYGSNGAATESVEYDSSFLMG
                     TGASSIDVKRYSQGSPTPPGVYNVRVFINQQSVASLELPFVDIGENSAAACITLKNLA
                     QLHIKKPEYPITLLAREGEEGDCLDIKKSIEQAEVSYDGSEQHLEISVPQAYVYKTYG
                     GYVDPSLWESGINATMLSYSLNAYHSDSKNGNRDSIYGAFNTGLNLGAWHLRARGNYN
                     WSQDNGNSFDFQDRYLQRDIPAIRSQVIVGDAYTTGETFDSVNVRGMRLYSDSRMLPS
                     ALASYAPIIRGVANSNAKVTVTQNGYKIYESTVPPGEFVIDDLSPSGFGSELVITIEE
                     ADGSKRSFTQPFSSVVQLQRPGVGRWDISAGQIIDDSLRHEPNMAQASFYYGFNNLFT
                     GYTGVQITDNDYMSGLLGLGINTSIGAFAVDITHARTEIPDDKTYQGQSYRITWNKLI
                     EATSTSFNLAAYRYSTEDYLGLHDALALIDDANHLSVNDDKDTIRTYSRMKNQFTVSV
                     NQPLSFAYEDYGSLFLSGSWTNYWAGNNNRTEYNVGYSKSVSWGNFSVNLQRSWNEDG
                     NKDDAMYLNVSVPLENIFGGKRKSSGFRNLSTQFNTDFNGSHQLNVSSSGNSEDNLIG
                     YSVNTGYNLDKESENVASIGGYLSYDSTWGGFSASASASTDNRQQYSVSTDGGFVLHS
                     GGLTFTNNSFGSNDTLVVIKAPGAKGARVNNGTDEIDRWGYAVSSSLSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSIILASFETDQGRSAVLNISTSNGKPIPFAAEVYQD
                     EIMIGSMGQGGQAFVRGINDSGELIIRWFEDSRTINCKLHYQLPAQPETLGSTNTLLL
                     NNLTCKLVNH"
     gene            4260..4817
                     /locus_tag="OHN27_000004"
     CDS             4260..4817
                     /locus_tag="OHN27_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2284519.1"
                     /translation="MKRIIIISTLVGLGLPTTGSATNINVDFTAQLLATTCTITIKED
                     GGPAVNNTGGDDYSMTIPTVGLDKIANAHADAQANFQLVAKDCSEGYSKIITKLSGAT
                     ISGNLIKNEATDTPVATNIGMGIKRRDAADASFITPNNSASVEWTTEDKTNGMKLTVA
                     LRETTAGTGNIGNFRAKATFNFTYE"
     gene            4832..5428
                     /locus_tag="OHN27_000005"
     CDS             4832..5428
                     /locus_tag="OHN27_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2284520.1"
                     /translation="MKLKAIAILIVTGGMISHSALAGTTSATLTVDSIVTMGTCTAQL
                     LDSNDKEISQVAIGDVYISELRNETNKKAFKIRFSGCAGLPQKKALVKLTPRGIGCGG
                     YNANSAGFANAKNGAGAAGRVAVEVWTTKNAEIEAGSEIFNCFSPADKTVNLSSVTAT
                     QHFDYDLSAKMVVAEGRQLSDVTPGEFLSPATFTISYQ"
     gene            5449..6039
                     /locus_tag="OHN27_000006"
     CDS             5449..6039
                     /locus_tag="OHN27_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2284521.1"
                     /translation="MLFQEHNFALFFGLVTALCSPVSLANKDVDLTANIVSSTCQIEI
                     NNNGVVDLGTVTLDYFSNNITPDTDYAGGKNFTVNVVSCDNLQTTQSQIKFDFQPQTG
                     SLAQANQQVFSNQYELQSIGAKNVGVVIFSAQPHAQKFNVRGTDGASKAIYALPSNQV
                     VPSTWTFYARMQKVNNALAPVAGIVRSQVIVNVYYE"
     gene            6051..7163
                     /locus_tag="OHN27_000007"
     CDS             6051..7163
                     /locus_tag="OHN27_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2284522.1"
                     /translation="MQGKVKKILLHIYSAVLLFLASLWYVFAGTGSECYIESSSAGSY
                     TIFINTPPYSVATQQVTTPYEISDAATDVPIVLRGDGIACKAIPNGDESIHFLNTADA
                     NLISTYTSARGSTLLKTTVPGIAYTVELICDTCPEVVDLRIPPAGDDNFTPNSDIWWV
                     WGETDEKWKLRFRFFYTPEFKPKNGVTSGTLLPGKIASWYIGINTQPWINFYVDADTF
                     KFTVDQPTCSTIALDSTSQNISGNKINLGDYYVSQVKQEITQVVPFTIRGDYCYASKI
                     TVKLKATTEAPNKKLIGKSSGSATGVGVKVYSTANNSEVQLNADNSNEIIFNYANWSN
                     NLLYFPFTAQLVKDGSNGTISPGDFTGNATFSFSYE"
     gene            7297..8091
                     /gene="panB"
                     /locus_tag="OHN27_000008"
     CDS             7297..8091
                     /gene="panB"
                     /locus_tag="OHN27_000008"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCQ2284523.1"
                     /translation="MKPTTISLLQKYKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8103..8954
                     /gene="panC"
                     /locus_tag="OHN27_000009"
     CDS             8103..8954
                     /gene="panC"
                     /locus_tag="OHN27_000009"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCQ2284524.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQVG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(9028..9930)
                     /gene="rpnC"
                     /locus_tag="OHN27_000010"
     CDS             complement(9028..9930)
                     /gene="rpnC"
                     /locus_tag="OHN27_000010"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCQ2284525.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10204..10584
                     /gene="panD"
                     /locus_tag="OHN27_000011"
     CDS             10204..10584
                     /gene="panD"
                     /locus_tag="OHN27_000011"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCQ2284526.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10588..11817)
                     /locus_tag="OHN27_000012"
     CDS             complement(10588..11817)
                     /locus_tag="OHN27_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCQ2284527.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLSVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDNAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11881..12321)
                     /locus_tag="OHN27_000013"
     CDS             complement(11881..12321)
                     /locus_tag="OHN27_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCQ2284528.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12426..13196)
                     /locus_tag="OHN27_000014"
     CDS             complement(12426..13196)
                     /locus_tag="OHN27_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2284529.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13193..14119)
                     /locus_tag="OHN27_000015"
     CDS             complement(13193..14119)
                     /locus_tag="OHN27_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2284530.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPRLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14228..14890
                     /gene="can"
                     /locus_tag="OHN27_000016"
     CDS             14228..14890
                     /gene="can"
                     /locus_tag="OHN27_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCQ2284531.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14931..15467)
                     /gene="hpt"
                     /locus_tag="OHN27_000017"
     CDS             complement(14931..15467)
                     /gene="hpt"
                     /locus_tag="OHN27_000017"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCQ2284532.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15673..18063
                     /gene="gcd"
                     /locus_tag="OHN27_000018"
     CDS             15673..18063
                     /gene="gcd"
                     /locus_tag="OHN27_000018"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCQ2284533.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18265..19815)
                     /gene="cueO"
                     /locus_tag="OHN27_000019"
     CDS             complement(18265..19815)
                     /gene="cueO"
                     /locus_tag="OHN27_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCQ2284534.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19981..20328
                     /locus_tag="OHN27_000020"
     CDS             19981..20328
                     /locus_tag="OHN27_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCQ2284535.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20434..21300
                     /gene="speE"
                     /locus_tag="OHN27_000021"
     CDS             20434..21300
                     /gene="speE"
                     /locus_tag="OHN27_000021"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCQ2284536.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21316..22110
                     /gene="speD"
                     /locus_tag="OHN27_000022"
     CDS             21316..22110
                     /gene="speD"
                     /locus_tag="OHN27_000022"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCQ2284537.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22148..22510)
                     /locus_tag="OHN27_000023"
     CDS             complement(22148..22510)
                     /locus_tag="OHN27_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCQ2284538.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22686..25283)
                     /gene="acnB"
                     /locus_tag="OHN27_000024"
     CDS             complement(22686..25283)
                     /gene="acnB"
                     /locus_tag="OHN27_000024"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCQ2284539.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25638..27491
                     /locus_tag="OHN27_000025"
     CDS             25638..27491
                     /locus_tag="OHN27_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCQ2284540.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTFPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAANQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFHDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPKIQQRREAARERIQSASPEQRQ
                     AVREKMQTNPQNQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRLER"
     gene            complement(27562..28986)
                     /gene="lpdA"
                     /locus_tag="OHN27_000026"
     CDS             complement(27562..28986)
                     /gene="lpdA"
                     /locus_tag="OHN27_000026"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCQ2284541.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29311..31203)
                     /gene="aceF"
                     /locus_tag="OHN27_000027"
     CDS             complement(29311..31203)
                     /gene="aceF"
                     /locus_tag="OHN27_000027"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCQ2284542.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31218..33881)
                     /gene="aceE"
                     /locus_tag="OHN27_000028"
     CDS             complement(31218..33881)
                     /gene="aceE"
                     /locus_tag="OHN27_000028"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCQ2284543.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34042..34806)
                     /gene="pdhR"
                     /locus_tag="OHN27_000029"
     CDS             complement(34042..34806)
                     /gene="pdhR"
                     /locus_tag="OHN27_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCQ2284544.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35347..36720
                     /gene="aroP"
                     /locus_tag="OHN27_000030"
     CDS             35347..36720
                     /gene="aroP"
                     /locus_tag="OHN27_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCQ2284545.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36763..37617)
                     /gene="ampE"
                     /locus_tag="OHN27_000031"
     CDS             complement(36763..37617)
                     /gene="ampE"
                     /locus_tag="OHN27_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCQ2284546.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37614..38165)
                     /gene="ampD"
                     /locus_tag="OHN27_000032"
     CDS             complement(37614..38165)
                     /gene="ampD"
                     /locus_tag="OHN27_000032"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCQ2284547.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            38253..39146
                     /gene="nadC"
                     /locus_tag="OHN27_000033"
     CDS             38253..39146
                     /gene="nadC"
                     /locus_tag="OHN27_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCQ2284548.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39349..39789
                     /gene="ppdD"
                     /locus_tag="OHN27_000034"
     CDS             39349..39789
                     /gene="ppdD"
                     /locus_tag="OHN27_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCQ2284549.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTLGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            39799..41184
                     /gene="gspE"
                     /locus_tag="OHN27_000035"
     CDS             39799..41184
                     /gene="gspE"
                     /locus_tag="OHN27_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCQ2284550.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLRNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCIHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41174..42376
                     /gene="hofC"
                     /locus_tag="OHN27_000036"
     CDS             41174..42376
                     /gene="hofC"
                     /locus_tag="OHN27_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCQ2284551.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELAHQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWMQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42411..43454)
                     /gene="guaC"
                     /locus_tag="OHN27_000037"
     CDS             complement(42411..43454)
                     /gene="guaC"
                     /locus_tag="OHN27_000037"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCQ2284552.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(43610..43654)
                     /locus_tag="OHN27_000038"
     CDS             complement(43610..43654)
                     /locus_tag="OHN27_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2284553.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43679..44299
                     /gene="coaE"
                     /locus_tag="OHN27_000039"
     CDS             43679..44299
                     /gene="coaE"
                     /locus_tag="OHN27_000039"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCQ2284554.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44299..45042
                     /gene="zapD"
                     /locus_tag="OHN27_000040"
     CDS             44299..45042
                     /gene="zapD"
                     /locus_tag="OHN27_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCQ2284555.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            45052..45249
                     /gene="yacG"
                     /locus_tag="OHN27_000041"
     CDS             45052..45249
                     /gene="yacG"
                     /locus_tag="OHN27_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCQ2284556.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(45349..45738)
                     /gene="mutT"
                     /locus_tag="OHN27_000042"
     CDS             complement(45349..45738)
                     /gene="mutT"
                     /locus_tag="OHN27_000042"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCQ2284557.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGKPWGKEGQPG
                     EWMALVDLNADDFPPANEPVIAKLKRL"
     gene            complement(45798..48503)
                     /gene="secA"
                     /locus_tag="OHN27_000043"
     CDS             complement(45798..48503)
                     /gene="secA"
                     /locus_tag="OHN27_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCQ2284558.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(48565..49077)
                     /gene="secM"
                     /locus_tag="OHN27_000044"
     CDS             complement(48565..49077)
                     /gene="secM"
                     /locus_tag="OHN27_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCQ2284559.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(49308..50225)
                     /gene="lpxC"
                     /locus_tag="OHN27_000045"
     CDS             complement(49308..50225)
                     /gene="lpxC"
                     /locus_tag="OHN27_000045"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCQ2284560.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(50326..51477)
                     /gene="ftsZ"
                     /locus_tag="OHN27_000046"
     CDS             complement(50326..51477)
                     /gene="ftsZ"
                     /locus_tag="OHN27_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCQ2284561.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(51538..52800)
                     /gene="ftsA"
                     /locus_tag="OHN27_000047"
     CDS             complement(51538..52800)
                     /gene="ftsA"
                     /locus_tag="OHN27_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCQ2284562.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(52797..53627)
                     /gene="ftsQ"
                     /locus_tag="OHN27_000048"
     CDS             complement(52797..53627)
                     /gene="ftsQ"
                     /locus_tag="OHN27_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCQ2284563.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(53629..54549)
                     /gene="ddlB"
                     /locus_tag="OHN27_000049"
     CDS             complement(53629..54549)
                     /gene="ddlB"
                     /locus_tag="OHN27_000049"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCQ2284564.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYEYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(54542..56017)
                     /gene="murC"
                     /locus_tag="OHN27_000050"
     CDS             complement(54542..56017)
                     /gene="murC"
                     /locus_tag="OHN27_000050"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCQ2284565.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(56071..57138)
                     /gene="murG"
                     /locus_tag="OHN27_000051"
     CDS             complement(56071..57138)
                     /gene="murG"
                     /locus_tag="OHN27_000051"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCQ2284566.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRVARA"
     gene            complement(57135..58379)
                     /gene="ftsW"
                     /locus_tag="OHN27_000052"
     CDS             complement(57135..58379)
                     /gene="ftsW"
                     /locus_tag="OHN27_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCQ2284567.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(58379..59695)
                     /gene="murD"
                     /locus_tag="OHN27_000053"
     CDS             complement(58379..59695)
                     /gene="murD"
                     /locus_tag="OHN27_000053"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCQ2284568.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFYRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSNLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(59698..60780)
                     /gene="mraY"
                     /locus_tag="OHN27_000054"
     CDS             complement(59698..60780)
                     /gene="mraY"
                     /locus_tag="OHN27_000054"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCQ2284569.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(60774..62132)
                     /gene="murF"
                     /locus_tag="OHN27_000055"
     CDS             complement(60774..62132)
                     /gene="murF"
                     /locus_tag="OHN27_000055"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCQ2284570.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(62129..63616)
                     /gene="murE"
                     /locus_tag="OHN27_000056"
     CDS             complement(62129..63616)
                     /gene="murE"
                     /locus_tag="OHN27_000056"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCQ2284571.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(63603..65369)
                     /gene="ftsI"
                     /locus_tag="OHN27_000057"
     CDS             complement(63603..65369)
                     /gene="ftsI"
                     /locus_tag="OHN27_000057"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCQ2284572.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(65385..65750)
                     /gene="ftsL"
                     /locus_tag="OHN27_000058"
     CDS             complement(65385..65750)
                     /gene="ftsL"
                     /locus_tag="OHN27_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCQ2284573.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(65747..66688)
                     /gene="rsmH"
                     /locus_tag="OHN27_000059"
     CDS             complement(65747..66688)
                     /gene="rsmH"
                     /locus_tag="OHN27_000059"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCQ2284574.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(66690..67148)
                     /gene="mraZ"
                     /locus_tag="OHN27_000060"
     CDS             complement(66690..67148)
                     /gene="mraZ"
                     /locus_tag="OHN27_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCQ2284575.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(67750..68754)
                     /gene="cra"
                     /locus_tag="OHN27_000061"
     CDS             complement(67750..68754)
                     /gene="cra"
                     /locus_tag="OHN27_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCQ2284576.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(68836..68922)
                     /locus_tag="OHN27_000062"
     CDS             complement(68836..68922)
                     /locus_tag="OHN27_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCQ2284577.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(68934..69425)
                     /gene="ilvN"
                     /locus_tag="OHN27_000063"
     CDS             complement(68934..69425)
                     /gene="ilvN"
                     /locus_tag="OHN27_000063"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCQ2284578.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSGKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(69428..71152)
                     /gene="ilvI"
                     /locus_tag="OHN27_000064"
     CDS             complement(69428..71152)
                     /gene="ilvI"
                     /locus_tag="OHN27_000064"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCQ2284579.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVCSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            71271..71315
                     /locus_tag="OHN27_000065"
     CDS             71271..71315
                     /locus_tag="OHN27_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2284580.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(71470..72414)
                     /gene="leuO"
                     /locus_tag="OHN27_000066"
     CDS             complement(71470..72414)
                     /gene="leuO"
                     /locus_tag="OHN27_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCQ2284581.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            73075..73161
                     /gene="leuL"
                     /locus_tag="OHN27_000067"
     CDS             73075..73161
                     /gene="leuL"
                     /locus_tag="OHN27_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCQ2284582.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            73254..74825
                     /gene="leuA"
                     /locus_tag="OHN27_000068"
     CDS             73254..74825
                     /gene="leuA"
                     /locus_tag="OHN27_000068"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCQ2284583.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            74825..75916
                     /gene="leuB"
                     /locus_tag="OHN27_000069"
     CDS             74825..75916
                     /gene="leuB"
                     /locus_tag="OHN27_000069"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCQ2284584.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            75919..77319
                     /gene="leuC"
                     /locus_tag="OHN27_000070"
     CDS             75919..77319
                     /gene="leuC"
                     /locus_tag="OHN27_000070"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCQ2284585.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAIAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            77330..77935
                     /gene="leuD"
                     /locus_tag="OHN27_000071"
     CDS             77330..77935
                     /gene="leuD"
                     /locus_tag="OHN27_000071"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCQ2284586.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(77984..79162)
                     /gene="setA"
                     /locus_tag="OHN27_000072"
     CDS             complement(77984..79162)
                     /gene="setA"
                     /locus_tag="OHN27_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCQ2284587.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLILFHSRLALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWIIAVISVVALFLTAKVKDI
                     "
     gene            complement(79264..79395)
                     /gene="sgrT"
                     /locus_tag="OHN27_000073"
     CDS             complement(79264..79395)
                     /gene="sgrT"
                     /locus_tag="OHN27_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCQ2284588.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            79484..81139
                     /gene="sgrR"
                     /locus_tag="OHN27_000074"
     CDS             79484..81139
                     /gene="sgrR"
                     /locus_tag="OHN27_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCQ2284589.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEIDYDQWHTGEIES
                     DIWLNSANFTLPLDFSVFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLLHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      81173..81272
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            81303..82286
                     /gene="thiB"
                     /locus_tag="OHN27_000075"
     CDS             81303..82286
                     /gene="thiB"
                     /locus_tag="OHN27_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCQ2284590.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAINVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDTPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            82262..83872
                     /gene="thiP"
                     /locus_tag="OHN27_000076"
     CDS             82262..83872
                     /gene="thiP"
                     /locus_tag="OHN27_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCQ2284591.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFIEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            83856..84554
                     /gene="thiQ"
                     /locus_tag="OHN27_000077"
     CDS             83856..84554
                     /gene="thiQ"
                     /locus_tag="OHN27_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCQ2284592.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(84668..85432)
                     /locus_tag="OHN27_000078"
     CDS             complement(84668..85432)
                     /locus_tag="OHN27_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2284593.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(85518..86396)
                     /gene="araC"
                     /locus_tag="OHN27_000079"
     CDS             complement(85518..86396)
                     /gene="araC"
                     /locus_tag="OHN27_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCQ2284594.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            86735..88435
                     /gene="araB"
                     /locus_tag="OHN27_000080"
     CDS             86735..88435
                     /gene="araB"
                     /locus_tag="OHN27_000080"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCQ2284595.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRSPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            88446..89948
                     /gene="araA"
                     /locus_tag="OHN27_000081"
     CDS             88446..89948
                     /gene="araA"
                     /locus_tag="OHN27_000081"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCQ2284596.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAELVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            90233..90928
                     /gene="araD"
                     /locus_tag="OHN27_000082"
     CDS             90233..90928
                     /gene="araD"
                     /locus_tag="OHN27_000082"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCQ2284597.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            91003..93354
                     /gene="polB"
                     /locus_tag="OHN27_000083"
     CDS             91003..93354
                     /gene="polB"
                     /locus_tag="OHN27_000083"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCQ2284598.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVGGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQVG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            93519..96425
                     /gene="rapA"
                     /locus_tag="OHN27_000084"
     CDS             93519..96425
                     /gene="rapA"
                     /locus_tag="OHN27_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCQ2284599.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            96437..97096
                     /gene="rluA"
                     /locus_tag="OHN27_000085"
     CDS             96437..97096
                     /gene="rluA"
                     /locus_tag="OHN27_000085"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCQ2284600.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(97213..98028)
                     /gene="djlA"
                     /locus_tag="OHN27_000086"
     CDS             complement(97213..98028)
                     /gene="djlA"
                     /locus_tag="OHN27_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCQ2284601.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            98283..100637
                     /gene="lptD"
                     /locus_tag="OHN27_000087"
     CDS             98283..100637
                     /gene="lptD"
                     /locus_tag="OHN27_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCQ2284602.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            100690..101976
                     /gene="surA"
                     /locus_tag="OHN27_000088"
     CDS             100690..101976
                     /gene="surA"
                     /locus_tag="OHN27_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCQ2284603.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            101976..102965
                     /gene="pdxA"
                     /locus_tag="OHN27_000089"
     CDS             101976..102965
                     /gene="pdxA"
                     /locus_tag="OHN27_000089"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCQ2284604.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            102962..103783
                     /gene="rsmA"
                     /locus_tag="OHN27_000090"
     CDS             102962..103783
                     /gene="rsmA"
                     /locus_tag="OHN27_000090"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCQ2284605.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            103786..104163
                     /gene="apaG"
                     /locus_tag="OHN27_000091"
     CDS             103786..104163
                     /gene="apaG"
                     /locus_tag="OHN27_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCQ2284606.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            104170..105012
                     /gene="apaH"
                     /locus_tag="OHN27_000092"
     CDS             104170..105012
                     /gene="apaH"
                     /locus_tag="OHN27_000092"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCQ2284607.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(105090..105569)
                     /gene="folA"
                     /locus_tag="OHN27_000093"
     CDS             complement(105090..105569)
                     /gene="folA"
                     /locus_tag="OHN27_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCQ2284608.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(105761..107623)
                     /gene="kefC"
                     /locus_tag="OHN27_000094"
     CDS             complement(105761..107623)
                     /gene="kefC"
                     /locus_tag="OHN27_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCQ2284609.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLTSSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEKAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(107616..108146)
                     /gene="kefF"
                     /locus_tag="OHN27_000095"
     CDS             complement(107616..108146)
                     /gene="kefF"
                     /locus_tag="OHN27_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCQ2284610.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(108254..109586)
                     /locus_tag="OHN27_000096"
                     /pseudo
     CDS             complement(108254..109586)
                     /locus_tag="OHN27_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(109645..109932)
                     /gene="fixX"
                     /locus_tag="OHN27_000097"
     CDS             complement(109645..109932)
                     /gene="fixX"
                     /locus_tag="OHN27_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCQ2284611.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(109929..111215)
                     /locus_tag="OHN27_000098"
     CDS             complement(109929..111215)
                     /locus_tag="OHN27_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCQ2284612.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(111266..112207)
                     /locus_tag="OHN27_000099"
     CDS             complement(111266..112207)
                     /locus_tag="OHN27_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCQ2284613.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(112222..112992)
                     /locus_tag="OHN27_000100"
     CDS             complement(112222..112992)
                     /locus_tag="OHN27_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCQ2284614.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            113465..114979
                     /gene="caiT"
                     /locus_tag="OHN27_000101"
                     /pseudo
     CDS             113465..114979
                     /gene="caiT"
                     /locus_tag="OHN27_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
     gene            115010..116152
                     /gene="caiA"
                     /locus_tag="OHN27_000102"
     CDS             115010..116152
                     /gene="caiA"
                     /locus_tag="OHN27_000102"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCQ2284615.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            116281..117498
                     /gene="caiB"
                     /locus_tag="OHN27_000103"
     CDS             116281..117498
                     /gene="caiB"
                     /locus_tag="OHN27_000103"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCQ2284616.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLATHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            117572..119125
                     /gene="caiC"
                     /locus_tag="OHN27_000104"
     CDS             117572..119125
                     /gene="caiC"
                     /locus_tag="OHN27_000104"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCQ2284617.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNCPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            119234..120019
                     /gene="caiD"
                     /locus_tag="OHN27_000105"
     CDS             119234..120019
                     /gene="caiD"
                     /locus_tag="OHN27_000105"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCQ2284618.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            120025..120615
                     /gene="caiE"
                     /locus_tag="OHN27_000106"
     CDS             120025..120615
                     /gene="caiE"
                     /locus_tag="OHN27_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCQ2284619.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(120824..121219)
                     /gene="caiF"
                     /locus_tag="OHN27_000107"
     CDS             complement(120824..121219)
                     /gene="caiF"
                     /locus_tag="OHN27_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCQ2284620.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(121481..124702)
                     /gene="carB"
                     /locus_tag="OHN27_000108"
     CDS             complement(121481..124702)
                     /gene="carB"
                     /locus_tag="OHN27_000108"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCQ2284621.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGADRIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(124720..125868)
                     /gene="carA"
                     /locus_tag="OHN27_000109"
     CDS             complement(124720..125868)
                     /gene="carA"
                     /locus_tag="OHN27_000109"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCQ2284622.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(126324..127145)
                     /gene="dapB"
                     /locus_tag="OHN27_000110"
     CDS             complement(126324..127145)
                     /gene="dapB"
                     /locus_tag="OHN27_000110"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCQ2284623.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLELHSL"
     gene            complement(127312..128226)
                     /gene="rihC"
                     /locus_tag="OHN27_000111"
     CDS             complement(127312..128226)
                     /gene="rihC"
                     /locus_tag="OHN27_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCQ2284624.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLEVP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPHIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(128292..129242)
                     /gene="ispH"
                     /locus_tag="OHN27_000112"
                     /gene_synonym="lytB"
     CDS             complement(128292..129242)
                     /gene="ispH"
                     /locus_tag="OHN27_000112"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCQ2284625.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKNAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(129244..129693)
                     /gene="fkpB"
                     /locus_tag="OHN27_000113"
     CDS             complement(129244..129693)
                     /gene="fkpB"
                     /locus_tag="OHN27_000113"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCQ2284626.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINDDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(129818..130312)
                     /gene="lspA"
                     /locus_tag="OHN27_000114"
     CDS             complement(129818..130312)
                     /gene="lspA"
                     /locus_tag="OHN27_000114"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCQ2284627.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGGWHFATFNLADTAICVGAAMIVLEGFLPSK
                     AKKQ"
     gene            complement(130312..133128)
                     /gene="ileS"
                     /locus_tag="OHN27_000115"
     CDS             complement(130312..133128)
                     /gene="ileS"
                     /locus_tag="OHN27_000115"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCQ2284628.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVNGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(133171..134112)
                     /gene="ribF"
                     /locus_tag="OHN27_000116"
     CDS             complement(133171..134112)
                     /gene="ribF"
                     /locus_tag="OHN27_000116"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCQ2284629.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(134120..134338)
                     /locus_tag="OHN27_000117"
     CDS             complement(134120..134338)
                     /locus_tag="OHN27_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCQ2284630.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            134441..134704
                     /gene="rpsT"
                     /locus_tag="OHN27_000118"
     CDS             134441..134704
                     /gene="rpsT"
                     /locus_tag="OHN27_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCQ2284631.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        32886 a        36642 c        33807 g        31708 t
ORIGIN      
        1 atctaacttg acacccataa tcataaataa aacaaataaa ttcactgata ttcactaaaa
       61 aatagactat acacaaaaaa atccataaac caaataaata tattcagcca agtgatactt
      121 atcaaatact tacataataa ccacatattc agtaatatac gccagctata ttaactaata
      181 acttttttgt aaggacacat gtaatgaaaa aaacattact cgccgccgca ctggtgatgg
      241 tttctggttc cgctttggct gtagatggtg gtcatattga ttttaacggt ctggttcagt
      301 ctggtacttg taaagtcggc gtggtagacg cgggtatgca cggcattact accgatggcg
      361 tagtaacact ggataccgca aatgttactg ataactttgc ggaagttaac gcgactgcag
      421 ttggtttgtt gccaaaagaa tttatgattt ctgtaaattg tgaacctggt aagacagaag
      481 cctctttaac tatgggttcc gcaagctacg caaacactag tggtactttg aataacaata
      541 tgaacatcac tgtaaatggc attcagccag cagagaatgt gaatattgca gttcataaca
      601 tgaccaacaa atctggcgca gctgagatta aacaggtaca catgaacaac tctgcagaaa
      661 aacatctgct gactttggat gctaatggcg acggtcaata tgtgtttaat gcctcctatg
      721 taaaagcacc gaacagccag gatgtgactt ctggacacgt taccactaac gcactgtaca
      781 ccattactta taactaatgg tgcaataagc ataaggtttg cctgcgggca aaccttattt
      841 ttccgttttc acataattac acaatagcat ggagtgctta tgtttcccat tataaaaaaa
      901 accgtcagcg cgttatttat tagtacgttg ttaaccagtg cacctgcatt cgccgatatc
      961 ataatttccg gcactcgtat catatataat gcagataaaa aagatgttaa cgtccgtctg
     1021 gaaaacaaag gcaatcggcc cttgctaatt caaaactggt tggatacagg tgatgacaat
     1081 gcggatccca gccagatcaa agttccgttt acttctacgc cgcctgtatc tcgcattgaa
     1141 tctaaacgtg ggcaaacggt aaaggtgatg tttactggaa caacacaact tcctgcggac
     1201 cgggaaagtg ttttctggtt taacgtgctg gaagtcccgc ccaagccgaa agatgccgaa
     1261 tcagacaaaa acttattaca actggctttc cgtacccgta tcaaactttt ttatcgccca
     1321 agtggattaa aaggtgaacc cgctgaagca ccagccaaac tgacgtggaa gttaaataat
     1381 gcgcagcttc aggtaaacaa cccaacccct tactacgttt cattcaatga cgtcgcaatt
     1441 gaaagcggcg gtaagagcta caaagtcgac tccagcatgg ttgcgccttt tggtcaggtt
     1501 agttttgccg tgacagatat agcgagcagt gtgtcttcag gaaaagttat ttacaaagcc
     1561 attaatgatt atggtggcaa tattgatggc tccacatcgc tttaatcact aaacggatta
     1621 actttaaatc gccaggaacg caaccttgta tacgttaact catcagaaaa gccgtctacc
     1681 aaaaacaaca cttctggctg catgttgtgc ttttttttat ggcagtaacg gcgcggcaac
     1741 agaaagcgtg gaatatgaca gttccttttt gatgggtact ggggcatcga gcatcgatgt
     1801 caaacgttat tcacagggta gcccaacgcc tcctggcgtt tataacgtcc gtgtgtttat
     1861 caaccaacaa tccgttgcca gtttagaact tccttttgtt gatatagggg aaaacagcgc
     1921 cgcggcctgt ataacactta agaatctggc acaactgcat attaagaagc cagaataccc
     1981 aattacactg cttgccagag aaggcgaaga aggcgattgc ctggacatca aaaaatcgat
     2041 cgagcaagcg gaagtcagct atgacggcag tgagcagcat ttggaaatct cggttccgca
     2101 ggcttacgtc tataaaacat atggcggtta tgttgacccc tcactttggg agtcgggtat
     2161 taatgccacc atgctttctt actcattaaa cgcctaccac tccgactcta aaaatggcaa
     2221 ccgggacagt atatacggag ccttcaatac cggtctgaat ttaggagcct ggcacctgcg
     2281 tgcgcgcggc aactataact ggtcacaaga taacggtaac agttttgact ttcaggatcg
     2341 ttacttacag cgtgacatcc cggcaatacg ctcacaagtg atcgtgggtg atgcctatac
     2401 caccggtgaa accttcgact cagtcaatgt acgtggtatg cgtctttata gtgatagccg
     2461 tatgctacca tccgcactgg ccagctatgc gccaatcatt cgtggcgtag caaattctaa
     2521 cgccaaagtg acagtgaccc agaacggtta caaaatctat gagtccaccg ttccaccggg
     2581 tgaattcgtg attgacgatc tcagtccttc agggtttggt agcgaattgg tcattactat
     2641 tgaagaagca gatggttcta aacgctcatt tacccaacct ttctcttctg tcgtgcagtt
     2701 gcagcgccct ggcgttggcc gttgggatat tagtgccgga caaattatcg atgacagctt
     2761 gcgtcatgaa cccaatatgg cacaggcctc tttttactac ggtttcaata atttattcac
     2821 cggttatacc ggagtccaaa ttacagataa cgactatatg tctggccttt tggggctagg
     2881 gatcaatacc agtattggtg cctttgcggt agatattaca catgcccgta ccgaaattcc
     2941 tgacgataaa acatatcagg ggcaaagtta tcgtataacc tggaacaaat taatcgaagc
     3001 tacaagcacc tcatttaacc ttgctgccta tcgttattcc actgaggatt accttggtct
     3061 gcatgatgcg ttagccctca ttgacgacgc caatcatctg tcagtcaacg acgacaaaga
     3121 cacgatccgt acctattcac gcatgaaaaa ccaatttacc gttagtgtaa atcagccgtt
     3181 gagttttgct tatgaggatt acggttcact ctttttatct ggcagctgga caaactactg
     3241 ggctggtaac aataaccgaa ctgaatacaa tgttggttac agtaaaagtg tctcctgggg
     3301 gaatttcagc gtcaacttgc aacgtagctg gaatgaggac ggcaataaag atgatgcgat
     3361 gtatttgaac gttagcgttc cgctggaaaa tattttcggc ggtaaacgta aatcctcagg
     3421 gttccgtaac ctaagcactc aattcaatac tgatttcaac ggttcgcatc aattaaacgt
     3481 cagtagttcg ggtaacagcg aagataatct catcggttac agtgtgaata cgggttataa
     3541 ccttgataaa gaatctgaaa atgtcgcttc cattggtgga tatcttagct atgactccac
     3601 atggggaggt ttttccgctt ctgcttctgc tagcaccgat aacagacaac aatactctgt
     3661 ctctaccgat ggtggttttg tacttcacag tggcggcctg acctttacca ataacagttt
     3721 cggtagcaat gacactttag tcgtcattaa agcacccgga gcgaaggggg cacgagtcaa
     3781 taacggtact gatgaaatcg atcgctgggg ttatgccgtt tcctcatcct taagtccata
     3841 ccgtgaaaac cgggtgggat taaatattga aacattggaa aacgatgtcg aactgaaaag
     3901 tactagtgcc accactgtac ctcgtagtgg ttccattatt cttgccagtt ttgaaactga
     3961 ccaggggcgt tctgcagttc tgaatatcag caccagtaac ggtaaaccta ttccttttgc
     4021 cgctgaagtt tatcaggatg aaattatgat cggcagtatg ggtcagggtg gtcaggcatt
     4081 tgtacgcggt attaatgaca gcggagagtt aattatccgc tggttcgaag acagtcgaac
     4141 aataaattgc aaattgcatt atcaacttcc tgcacagcca gaaacacttg gaagtacaaa
     4201 caccttatta ttaaacaacc ttacctgtaa gttggttaac cactaaaatg gaacattcta
     4261 tgaaaaggat cataataata tctacgctag ttggccttgg gttacccaca actggctcag
     4321 cgacaaatat taatgtcgat tttaccgcac aattgctggc gactacttgt acaataacga
     4381 ttaaagaaga tggaggcccc gcggttaata atacgggcgg tgatgattat agtatgacta
     4441 tcccaacggt tgggctggat aaaattgcca atgctcatgc tgatgctcaa gccaattttc
     4501 aattggtggc caaagattgt agtgagggtt attcgaaaat tatcactaaa ctttctggag
     4561 caaccatttc cggtaacttg attaaaaacg aagcgacgga cactcctgta gcaaccaata
     4621 ttggtatggg catcaagcgc cgggatgcag ctgatgcttc gtttattacg cctaataata
     4681 gtgcaagtgt tgagtggact acggaagata aaactaatgg aatgaagtta accgttgccc
     4741 tgcgcgaaac tacagcaggg acaggaaata tcggtaattt ccgtgcgaaa gccaccttta
     4801 acttcaccta cgaatgacag gaacagcgac catgaaatta aaagcaatag caatactgat
     4861 tgttactggt ggaatgatta gccatagtgc cctggcagga acaacgtctg caaccttaac
     4921 ggtagacagc attgtcacaa tgggtacttg tactgctcaa ttactggact ccaatgataa
     4981 agaaatctca caagttgcga ttggtgacgt ttatatttct gaactaagaa atgaaaccaa
     5041 taaaaaagcc tttaaaatcc gttttagtgg ttgtgccgga cttccgcaaa aaaaagcact
     5101 ggtaaaatta actcccagag gaataggttg cggtggttat aatgctaact cagccggttt
     5161 tgcaaatgca aagaatggtg cgggcgcagc aggaagagtt gcagttgaag tatggactac
     5221 caaaaatgca gaaattgaag ctggcagtga aatttttaat tgttttagcc ccgcagataa
     5281 aacggttaac ctttcttccg tcactgcgac gcaacatttc gattatgatt taagtgctaa
     5341 gatggttgtt gctgaaggca gacagttgag tgatgtcaca ccgggtgagt ttctttctcc
     5401 agctaccttt acaatctctt atcagtaaca acagataagg aaattataat gcttttccag
     5461 gaacataatt tcgcactttt tttcgggctg gtaacagccc tctgctctcc tgttagcctg
     5521 gctaataaag atgtagatct tactgcgaac attgtgtcaa gcacatgtca gatcgaaata
     5581 aataataatg gtgtagttga tctcggcacc gtgacactgg actatttttc taacaatata
     5641 accccagata cggattacgc tggcggtaag aattttactg tcaacgtcgt ttcttgtgat
     5701 aatttgcaaa ccacacaatc gcagattaaa ttcgattttc aaccacaaac tggatctctt
     5761 gcccaagcaa atcaacaagt tttcagtaac caatacgagt tacaatctat cggagctaaa
     5821 aatgttgggg ttgttatttt ttcagcacag ccccatgcac aaaaatttaa cgttcggggg
     5881 acggacggag catcaaaagc catttatgcc ttaccctcta atcaagtggt tccgtcaacg
     5941 tggacattct atgcccgaat gcaaaaagtc aataacgctc ttgcacctgt tgctggcatt
     6001 gtgagaagtc aggttatagt taacgtttat tacgaataat aaggattact atgcaaggga
     6061 aagtcaaaaa aattttgctt catatataca gtgcagtgct attatttttg gcatcgttat
     6121 ggtatgtttt tgctggcacg ggttctgaat gctatataga aagcagttcg gccgggagtt
     6181 ataccatttt cattaatacc cctccctata gtgtagcgac acagcaggtc actactcctt
     6241 acgaaatatc tgatgcagcc acagatgtac caattgtact tcggggtgat ggaattgcat
     6301 gtaaagccat accaaatggc gatgagagta tacattttct taataccgct gatgccaatc
     6361 taatatcaac atatacttca gcacgaggtt ctacattatt aaaaaccacc gttccgggta
     6421 ttgcttatac tgttgaatta atttgtgata cctgtcctga agtagtcgac ttacgcattc
     6481 caccagctgg cgacgataac ttcaccccca actccgatat ctggtgggtg tggggcgaaa
     6541 cagatgaaaa atggaagtta cgttttcgct ttttctatac tccggagttt aaacctaaaa
     6601 atggtgttac cagtggaacg ttattaccgg gaaaaattgc ttcctggtat attggtatca
     6661 acacccaacc atggatcaac ttttacgttg atgcagatac atttaaattt accgtggatc
     6721 aacctacatg tagtaccatt gctctggatt caaccagcca aaatatcagc ggtaataaaa
     6781 taaacttggg agactattat gtttcccagg ttaagcagga aatcacccaa gtagttccat
     6841 ttactattcg gggcgattac tgttacgcaa gcaaaattac agtgaagttg aaagccacaa
     6901 ctgaggcacc aaataaaaaa ttaattggaa aatcatccgg ctcagcaacc ggtgttggtg
     6961 taaaagtcta ctcaactgcg aacaacagtg aggttcagct aaacgcagac aacagcaacg
     7021 agattatatt taactacgct aactggtcaa acaaccttct ttattttccg tttacggcgc
     7081 aactggttaa agatggaagt aatggcacaa ttagccctgg ggattttacc ggtaatgcga
     7141 cattctcttt ttcctatgaa taagtaagtc tcaccccaac tcaacgcacc agattcaaac
     7201 tctggtgcga aactttcttc acactaaaaa ccattgcccc cctgatttcg actgttagaa
     7261 tgcaatcaga tctgacttta gctatcagga tacgttatga aaccaaccac catctcctta
     7321 ctgcagaagt acaaacagga aaaaaaacgt ttcgcgacca tcaccgctta tgactatagc
     7381 ttcgccaaac tctttgctga tgaaggactt aacgtcatgc tggtaggcga ttcgctgggc
     7441 atgacggttc agggacacga ctccaccctg ccagttaccg ttgccgatat cgcctaccac
     7501 actgccgccg tacgtcgcgg cgcaccaaac tgcctgctgt tggctgacct gccgtttatg
     7561 gcgtatgcca caccggaaca agcctttgaa aacgccgcaa cggttatgcg tgccggtgcc
     7621 aacatggtca aaattgaagg cggtgagtgg ctggtagaaa ccgtacaaat gctgaccgaa
     7681 cgtgccgttc ctgtatgtgg tcacttaggt ttaaccccac agtcagtgaa tattttcggt
     7741 ggctacaaag tacaggggcg cggcgatgaa gcgggtgatc aactgctcag cgatgcatta
     7801 gccttagaag ccgctggggc acagttgctg gtgctggaat gcgtgccggt tgaactggca
     7861 aaacgtatta ccgaagcact ggcgatcccg gttattggca ttggagcagg caacgtcact
     7921 gacgggcaga tcctcgtgat gcacgacgcc tttggtatta ccggcggtca cattcctaaa
     7981 ttcgctaaaa atttcctcgc cgaaacgggc gacatccgcg cggctgtgcg gcagtatatg
     8041 gctgaagtgg agtccggcgt ttatccgggc gaagaacaca gtttccatta aggagtcacg
     8101 ttgtgttaat tatcgaaacc ctgccgctgc tgcgtcagca aattcgccgc ctgcgtatgg
     8161 aaggcaagcg cgtggcgctg gtgcctacca tgggtaacct gcacgatggc catatgaagc
     8221 tggtcgacga agccaaagcc cgcgccgatg tggtcgtcgt cagtattttc gttaacccga
     8281 tgcagttcga ccgcccggaa gatctggctc gttatccacg caccttgcag gaggactgcg
     8341 aaaagctaaa caaacgtaaa gtggatttag ttttcgcccc ttcggtaaaa gagatctacc
     8401 cgaacggtac tgaaacccac acttacgttg acgttcctgg cctttcgacc atgctggaag
     8461 gtgccagccg tccgggacat tttcgcggcg tttcgactat tgtcagcaag ctgttcaacc
     8521 tggtccagcc ggacatcgcc tgcttcggtg aaaaagattt tcagcaactg gcgctgatcc
     8581 gcaaaatggt tgctgatatg ggctttgata ttgagattgt cggtgtgcca attatgcgcg
     8641 ccaaagacgg tctggcgctg agttcccgta acggttatct gacggcggaa caacgcaaaa
     8701 ttgcgcccgg tctgtacaaa gttttaagtt cgattgccga caaattgcag gttggcgaac
     8761 gggatctcga tgaaattatt actattgccg ggcaagaact gaatgaaaaa ggcttccgcg
     8821 ccgatgatat tcagattcgc gatgccgaca cgttgctgga agtctctgaa accagcaaac
     8881 gggctgtgat tctggtagcc gcctggcttg gcgatgcccg cctgatcgac aacaaaatag
     8941 tcgaactggc gtaatactta actggcgcta cggctgatgg cgccagttat taatttgccc
     9001 tacgtcatct gtcataacac aggtaactta aataaggtta attaccttat caatctcagc
     9061 aagaggtaaa tttgccatct ctgcaacgtc ttcccgagac attcctttac tcagaagacg
     9121 cagggcgaat tcttgccttt cctcctgtct tccctgctga atccccttct caatcccttt
     9181 ctcttcaaac cactgcgcca gcgtcatcat agacttccct cccgtttccc tgtctctcag
     9241 cacaccataa aacaaatccg cttgttcagt atgaccgcgt tgcagcatat agttttgcat
     9301 ggcaactaac tgacttccgc tagtgtaccc ttcgtcaatc agcgtgacca gttgctcaag
     9361 caataacatt aagtcgcgct ggcgaatatg tttttgcagt agttcgagaa tcgcaatccg
     9421 ccgatgttgc atgatttcgt catccggtgt gatggtgata tccaccagcg ggaaaggact
     9481 gttatagacg cgtcgcgcca gctccggcga gtaaaacata tcaaaccagc acattgatag
     9541 cggataaggt gtggcctcgc cctgataaaa cagtatcggc accaccagcg gcagcttatc
     9601 gtggtcagcc tccagatgcc ggtgcatggc ggctatagaa taacgcatca tgcgaaaggc
     9661 cattttctta tccggcttgc tttggtgttc aatcacaaca tgcagataac cgggattgcc
     9721 ctgcatttgc acggaataga gcacgtccgt gctgtgtcct ttcaggctct cttcaatgaa
     9781 actccccgac tctaaatgaa gcgtgttgag gtcacaaagt tcgcgtaatt ccactggcaa
     9841 atgtatctcc agaaagtcgc gagccgtctc cgcatgcatt aaaaattgtt taaataccgc
     9901 gtcatgcggt gtggtactcg gtgcatccat cttccctgtc ttccgtgtgg ctttgcgtga
     9961 aggcagtatc cagtcaaggc gcgctatcgt cagccacctc tttattgttt accgagcagc
    10021 gttcagagaa atatcaattc gttacaggcg atacatggca cgcttcggcg cggtacgcaa
    10081 actgctgacg caaatgccgt cagatgagta gacactaaac aaaaatcggg caatactgcg
    10141 tgagaatttc ctcaaagcag gcatcgcctg cttcgttaac gacagggtag aaaggtagaa
    10201 gttatgattc gcacgatgct gcagggcaaa ctccaccgcg tgaaagtgac tcatgcggac
    10261 ctgcactatg aaggttcttg cgccattgac caggattttc ttgacgcagc cggtattctc
    10321 gaaaacgaag ccattgatat ctggaatgtc accaacggca agcgtttctc cacttatgcc
    10381 atcgcggcag aacgcggttc gagaattatt tctgttaacg gtgcggcggc ccactgcgcc
    10441 agtgtcggcg atattgtcat catcgccagc ttcgttacca tgccagatga agaagctcgc
    10501 acctggcgac ccaacgtcgc ctattttgaa ggcgacaatg aaatgaaacg taccgcgaaa
    10561 gcgattccgg tacaggttgc ttgattgtta tccctgcggc tggttactca ccagccgcga
    10621 catcgtctcc agcgaatccg ttcttaagat ataaagtcgt tttagtaaca acggattatc
    10681 ccccggtttt actttgcctt tcatggttgt caccgccagg tgaaatccgg catcgtttgc
    10741 tgccttcacg gcgttgtcat taaatccgcc aaacggatac gaaagatacc agacatgcgg
    10801 attaaattgc gccagagcgc ggcgtgaacg tgcaaaatca aacagaatat tgtgctcact
    10861 acggctcagt aatatgggtc ggcgataacc atctacccga tgcaaaaaat gggtatgtga
    10921 ctggaaatca aatacatcgc gaatttcgtt aagctcagaa acgctcataa attgcagcga
    10981 ttttgggttc cacttctgcg ggtgacgttt gatgcgtgag gtaacaataa acgccgtcgc
    11041 cttcatgcca tattgtttca acacaggata cgcatagcgg ctcaccgact tgaggccatc
    11101 atcaaaggta atcaccaccg ctcgcgcagg gagattgatc ttattcttca cgtagccttc
    11161 cagctgcacc atgctcagtg tcgcgtatcc cctgtcacgc agccaggcca tctggttatt
    11221 gaaagcgcgt accgatgtgg tcgtcgaagt atggcgaaaa cgggtgtttt cttcgtcgcg
    11281 cagaatatgg tgataggtta gcaccgacag gccattatcg ggttgggcat ccagtgcgct
    11341 gatataggcc agtcgatcgc caatacggat ctgataccag gtttgattta acctgtcttt
    11401 cagtttatgc aaaatcgggt agcgcaaatt gtccgccagt accccaaatg gcgcacttcc
    11461 cgcactcggc gcgttataga ccggcgtatc tttccaggta actaagttct gattactcag
    11521 cggcttgttg aggtcgccca aaccgtcttc aactttttgt cgcccctgaa ccggctcgag
    11581 atgaccttta tcgataaaac ctttgccaaa gccaaaatta aatgcgtaat aacttgcggc
    11641 agtgggctcc acggcaatga tttgtccggc ccgaatattc cccacggtca ccatcttgtc
    11701 accaatttgc gcccagaccg cagcattttc gatggtttgc atataacggg caggtaacgc
    11761 ggcactgaca cttgcggtaa acagcatcag caggagaata acagcttgtt tgtacatgaa
    11821 aaaataaccg agggtgaaac aacgctttta ttgtagcaaa agcgccatca gaatcgcggg
    11881 ttaatactca tacaaattat gtttgaggac gaaaggcgga tttttttgtt gttggtgcca
    11941 aagactactc acctccggcg cacccagttc gacaatacgc tcgcgaaact ctgaactggt
    12001 catggtttca cggcaagcca tcatctgttc aattaacggt aacgtgacac cagaaatcac
    12061 ttcgcaacgg gagtgtttgt ggcttaataa tgaagccacg cgatacggtg gcgctccggc
    12121 tatatcggtt aagaagatga caccctcacc agagtccgct tcatgcagag catcgcacat
    12181 catgcggctg agcatattag agcttaatcc gcgccagaaa ttcacggccc ggcactgaag
    12241 aagtgcccca tgttttttct ccagcgcatc cagtatctct tgcgcccggt catcgtgaca
    12301 ggtaattacc caacctaaca tcgccttcct ccttagccga aaatcagtct accctgacca
    12361 ttgttgataa agggtgatag ccgtcaaaac tcctctcccc caaatccaga ggagaggaaa
    12421 tagccttagc tacgcaaacc acgtccacgt tggatcagcg accaacagat caaataaaac
    12481 gccacaataa agaccaccag tacgccaaag gtagtgacca gcggaacatc attgatacca
    12541 aggaagccgt agcggaaacc actgatcata taaacgattg ggttcaggtg cgacagccct
    12601 tgccagaacg gcggcaacaa agtcagggag taaaagaccc cgcccaaata cgtgagtggc
    12661 gttaacacaa aggttggcac caggctgatg tcatcgaacg ttttggcaaa cacgccgttc
    12721 agcaaacccg caagggagaa tagcaccgcc gtgagcacca gcgttaaggc aacgaatacc
    12781 catgaatgca cctgaaacgg cacaaaaaac agtgaaattg ccgtcaccag aatgccaaca
    12841 aacagaccac gcgccacgcc accgccgaca tatccggcaa taatgacgtg agtcggaacc
    12901 ggcgctacca gcagctcttc aatattacgc tggaacttgg caccaaaaaa tgatgacgcg
    12961 acgttagcgt aggcgttggt gatcaccgac atcatgatca gccccggtac gatgaactgc
    13021 atgtagctga agccatgcat atcgccaata cgcgaaccaa tcaggttacc gaagataata
    13081 aagtaaaggg tcatggtgat gactggcggc actagcgtct gcacccagat acgcataaag
    13141 cgatggatct ctttcgccca gatgcttttt agcgccaccc agtaaagatg catcatgcgc
    13201 gatctccttg tttttcatta accagtgaaa caaacagctc ttccagacgg ttagctttgt
    13261 tacgcatact taatacctga atgccctgct cacttaactg cgtaaatacg ctgttgatcc
    13321 cctgctcacg cagcacttca acttccagcg tcgcggtatc aaccagtcga tactgatagc
    13381 catcgagcct cggtaacgga cttttcggtg cgagatcgag aataaaggtt tccgatttca
    13441 gcttcgccag cagcgccttc atcgaggtat tttccaccag ctcaccgtgt tgaataatgc
    13501 cgatattgcg gcacagcatt tctgcttctt ccaggtagtg tgtggtgaga atgatggtgg
    13561 tgcctttgtc gtttaaatcc ttcaaaaagc cccacattga gcggcgaagc tcaatatcca
    13621 cgcctgcggt cggttcgtcg agaatcagta gtttaggctc atgcattaac gcacgggcaa
    13681 tcattaaacg gcgcttcatc ccgccagata acatacgcgc acgttcgttg cgttttcccc
    13741 ataaatcgag ctgcttaaga tacttttcgc tgcggatgta cgcttctttg cgctccacac
    13801 cgtagtaccc tgcctgatta accacaattt gctgcacggt ttcaaacggg ttgaagttaa
    13861 attcctgcgg caccagtccc aactgacgtt tagcgttcac gacatccttc tcgagatcgt
    13921 aaccaaatac gctgacccgc ccggaggttt tatttaccag agagctgata ataccgatag
    13981 tggtcgattt cccggccccg ttcggcccga gaagcgcata aaaatcaccc gcttcgacct
    14041 gcaaatctat tccacgaagc gcctgaacgc cgcctggata ggttttttta agctgttgaa
    14101 gttccagtgc aatggtcata aatttttact taccttacgt tcttacactt tatctgtggt
    14161 ttaaatcgtc ccggagttgc cctatattag ccaaacgtaa ttatttggtt acaggtcgtt
    14221 aacctccatg aaagacatag atacactcat cagcaacaat gcactatggt caaaaatgct
    14281 ggtggaagag gatcccgggt tttttgagaa actggcacaa gcgcaaaaac cgcgctttct
    14341 atggattgga tgttccgaca gtcgcgttcc tgcagaacgt ttaaccggtc ttgagccggg
    14401 cgaactcttt gttcaccgta atgttgctaa cctggtcatt cacaccgacc tgaactgcct
    14461 ttccgtggtt cagtatgcag tggatgtact cgaagttgaa cacattatta tctgtggcca
    14521 ctacggttgc ggcggcgtac aagccgccgt tgaaaacccg gaactggggc ttatcaacaa
    14581 ctggctgctg catatccgcg atatctggtt caaacatagc tcattgctcg gcgaaatgcc
    14641 gcaagagcgc cgtctggata ccttgtgtga actgaacgtc atggaacagg tttataacct
    14701 gggccactcc accattatgc aatcagcgtg gaaacgcggg cagaaagtta ccattcacgg
    14761 ctgggcctat ggcattcacg acggcttgct gcgtgatctg gatgtcacag cgactaaccg
    14821 cgaaaccctt gagcaacgtt accgtcacgg gatttccaac ctcaagctga aacacgccaa
    14881 ccacaaataa aaatgccatg ccggatgcaa cacatccggc aacttcacac ttactcgtcc
    14941 agcagaatca ctttgccgat atacggcaga tgacggtaac gctgtgcgta atcaatgccg
    15001 taacccacca caaactcatc cgggatcgag aaaccaataa attctaccgg gacgttcact
    15061 tcacgacggg acggtttatc cagcagcgta caaatcgcca gcgacttcgg ttcgcgcagg
    15121 cttaagatct cacgcacttt cgacagtgta ttccccgagt cgatgatatc ttcaacaatc
    15181 agcacgtcct tgccacggat atcttcatcc agatctttga ggattttcac atcacgggtg
    15241 gtggacatgc cgctaccgta gctggaggcg gtcataaagt cgacttcatg agatacctga
    15301 acttcacggc acaggtccgc cataaacata aatgagccac gcagcagacc aaccagcacc
    15361 atatcgctgc cgctgtcttt gtaacgctca gtaatctgac gacccagttc ggcgatacgc
    15421 gctttaatct ccgcttcggg gatcattact tctacagtat gtttcatatc tctaaccata
    15481 tgatttaaaa acaaatcact tcgcacgggc aggttttggc accgcaacaa agcgcaagac
    15541 acgcagtata ccagtaaaac tattcattcg cggaatctgt taataaagct cgaattgtga
    15601 tgacgatcac acatgttaaa acctataatt aattgctact aaaatattaa tgagttgaaa
    15661 tggtgtctct ttatggcaat taacaataca ggctcgcgac gattactcgt cacgctaaca
    15721 gccctttttg cagcgctttg cgggctgtat ctactcattg gcggaggctg gctggtcgcg
    15781 attggcggct cctggtacta ccctatcgct ggccttgtga tgctcggcgt cgcctggatg
    15841 ctgtggcgca gtaaacgtgc cgcgctttgg ctatacgccg ccctgctgct cggcaccatg
    15901 atttggggcg tctgggaagt tggtttcgac ttctgggcgc tgactccgcg cagcgatatt
    15961 ctggtcttct tcggcatctg gctgatcctg ccgtttgtct ggcgtcgcct ggtcattcct
    16021 gccagcggcg cagttgccgc actggtggtg gcactgctga ttagcggtag tatcctgacc
    16081 tgggccggat ttaacgatcc gcaggaaatc aacggcacct taagcgccga tgccacacct
    16141 gcggaagcta tctcacccgt tgccgatcag gactggcctg cctatggtcg caatcaggaa
    16201 ggtcaacgct tttcacctct gaaacaaatt aacgccgata acgtccataa tctgaaagaa
    16261 gcctgggtgt tccgtactgg cgatgtgaag cagccgaacg atccgggcga aatcaccaat
    16321 gaagtgacgc cgattaaagt gggcgacacc ctttacctgt gtaccgctca ccagcgcctg
    16381 tttgcgctcg atgccgccag cggcaaagag aaatggcatt acgatcctga gctgaaaact
    16441 aacgagtctt tccagcacgt aacctgccgt ggcgtctctt atcatgaagc caaagcagaa
    16501 acggcttcgc cggaagtgat ggcggattgc ccgcgtcgta tcattcttcc ggtcaatgat
    16561 ggtcgactga ttgcgattaa cgctgaaaac ggcaaactgt gcgaaacctt cgccaataaa
    16621 ggcgtgctca atctgcaaag caatatgcca gacaccaaac cgggtctgta tgaaccgact
    16681 tcgccaccga ttatcaccga taaaaccatc gtgatggcag gttcagttac cgataacttc
    16741 tcaacccgcg aaacgtctgg cgtgatccgt ggttttgatg tcaacaccgg ggagctgctg
    16801 tgggcttttg atcccggcgc gaaagatccg aacgcaatcc cgtctgacga acacaccttt
    16861 acctttaact cgccaaactc gtgggcacca gcggcctatg acgcgaagct ggatctggtg
    16921 tatctgccga tgggcgtgac cacgccggat atctggggcg gtaaccgcac accggaacag
    16981 gaacgttatg ccagctcgat tctggcgctg aatgccacta ccgggaaact ggcgtggagc
    17041 taccagaccg ttcaccacga cctgtgggat atggatcttc cggcacagcc gacgctggcg
    17101 gacatcaccg ttaatggtca gaaagtgcca gttatttacg ctccggcgaa aaccggcaac
    17161 atttttgtgc tcgatcgtcg taatggcgaa ctggtggttc cggcaccgga aaaaccggtt
    17221 ccccaaggtg ctgccaaagg cgattacgtt acccctactc aaccattctc ggaactgagc
    17281 ttccgtccga cgaaagattt gagcggtgcg gatatgtggg gagccaccat gtttgaccaa
    17341 ctggtgtgcc gcgtgatgtt ccaccagatg cgctatgaag gcattttcac cccgccatct
    17401 gaacagggta cgctggtctt cccgggtaac ctggggatgt tcgaatgggg cgggatttcc
    17461 gttgatccaa atcgtgaagt ggcgattgcc aacccaatgg cactgccgtt tgtttcgaaa
    17521 ctgatcccgc gtggtcctgg caacccgatg gagcagccga aagatgccaa aggcacgggt
    17581 acggaatccg gcattcagcc acagtacggt gtaccgtatg gtgtcacgct caacccgttc
    17641 ctctcaccat ttggtctgcc atgtaaacag ccagcatggg gttatatctc ggcgctggat
    17701 ctgaaaacta atgaagtggt gtggaagaaa cgtattggta cgccgcagga cagtatgccg
    17761 ttcccgatgc cggttccggt gccgttcaat atgggtatgc cgatgctggg cgggccaatc
    17821 tccacggcgg gtaacgtgct gtttatcgcc gctacggcag ataactacct gcgcgcttac
    17881 aacatgagca acggtgaaaa actgtggcag ggtcgtttac cagcgggtgg tcaggctacg
    17941 ccaatgacct atgaagtgaa tggtaagcag tatgtggtga tctccgcagg cggtcacggt
    18001 tcatttggta cgaagatggg cgactatatt gtggcttatg cgctgccgga tgatgtgaag
    18061 taagacttgc tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
    18121 ccttatccag cctacaaaat tgtgcaaaat caatggattg cacagctagc gtaggcctga
    18181 taagcgtagc gcatcaggca gatttgcgtt tgtcatcagt ttaatgcccg gagagatccg
    18241 ggcatatttc cgaatacggt ctttttatac cgtaaaccct aacatcatcc ccgtatcttc
    18301 atgctccagc agatggcagt gcgccatata agcatgttct ttcggtgcat cgtgattaaa
    18361 cttcaccagc acttcgctga cattaccttc taccttaacg gtatctttcc agcccgcgcg
    18421 atgagccgct ggcggtttgc cattttctga caagatacgg aactgcgtgc cgtggatatg
    18481 gaacggatgc agcatcatgt cgcccacgcc agagataacc caacgttcgt attgcccttt
    18541 cgccgccgca aacatcggct tgttcatatc aaacgcctga ccgttgattt tgttggcatg
    18601 gtggaaatcg aacttcccgc cgtggttcat atgattcata ttgccgtgcc ccatatggcc
    18661 catcatctgg ctgtgatcca tcccggccat cgcctgatcg ccatatttct ccattagcat
    18721 ctgcatcccc atcatatcga gcatcgggtc catagagagt tgcagcttgc gtaccgtcag
    18781 cccttccagc gaaggtaacg caggcaggct acttaatgtg tctggcaaag caccggaggc
    18841 actaatagca atcggctgaa tccgcattac cggatgaggc ttatcaaacg gcgcaatcgc
    18901 catccccatc tggctgaccg gcagcgtcac caggtcaaag ggtttgttat cgttaacctc
    18961 caccagcact tcaaaacgct cgcccatcag caccggcagt tcgctcacct tcactggttc
    19021 aggtagcaga ccaccgtcgc tggcaatcac atacagcggg cgattgtcgc tggtggcgaa
    19081 attgagcgaa cgggcattac agccattgag caaacgcagg cgcagccaac cacgcggggc
    19141 agcgtgttgc gggtagattg caccgttggt cagcaacgta tcgccaaacc agcccacggc
    19201 ggcggtcatc acatccagtt gataatcaat ctgcccgtcg gcgctaaatt tcttatcctg
    19261 aacgatcacc ggaacatcat cgatacccca ctgttttggc agcattaatt tcaggatctc
    19321 gtcatcttca atcaccacca gcccagccag ccccatcgcc acctgtcgcc cggttttgcc
    19381 gtgctgatgc ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga
    19441 gcgcttgcca cctggcggaa taattccctg cgggccgccg tcgacttcac ccggtacttc
    19501 cagcccgtgc cagtgcaacg ttgtctcttc cgtcagttgg ttgtagatat caaccgttac
    19561 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    19621 agttgcagtt ttcccgccaa aggtggactg gcctgcgcca atagttaact gaatgcgatt
    19681 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttctg ccgcaaatac
    19741 tgcgcggctc cacagcggca aagccgaagc cacacccagc gcgacggaat attttaagaa
    19801 atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg
    19861 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    19921 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    19981 atgaagacgt ttttcagaac agtgttattc ggcagcctga tggccgtctg cgcaaacagt
    20041 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    20101 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    20161 tttttcgctc agcaaaacca gtgggacctc agtaattacg acaccttcga catgaaagcc
    20221 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc
    20281 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    20341 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcctttt
    20401 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    20461 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    20521 accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    20581 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    20641 gttccgctac tggcccatgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    20701 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    20761 gatgcgggtg tcgtatcgtt ctgccgtcag tatctaccca accataacgc cggtagctac
    20821 gacgatccgc gctttaagct ggtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    20881 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctt
    20941 ttcacttcgg cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    21001 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    21061 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    21121 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    21181 attcaggcgc gttttctcgc ctctggcctg aaatgccgtt attacaatcc ggcaatccat
    21241 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gccgtcctaa
    21301 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    21361 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    21421 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    21481 tgttccatta tcggggctaa tattcttaac atcgcccgcc aggattacga accacagggt
    21541 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    21601 gaacaccccg gcccactgcc agaaacggtc gttgcccatc ttgataaaag tcatatttgc
    21661 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    21721 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    21781 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttacccg cgacattaac
    21841 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    21901 atgaaggcgc tgtatgacat ggtggatgtg aacgtctatc aggaaaatat cttccatacc
    21961 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagac
    22021 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    22081 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    22141 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    22201 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    22261 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcaccgtcc atccacaggg
    22321 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    22381 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    22441 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    22501 cgtaatccat agtgtgttcc tctgctcccg gcaatgcacc gaaatcgcat tgcgccattg
    22561 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    22621 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctg
    22681 actttttaaa ccgcagtctg gaaaatcacc ccatcggctt tctcggtgta ctgagaaagc
    22741 tggttgaagt tcagataacg gtaagtatca acggctgttt tatctacctg cgccacgtag
    22801 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttcc
    22861 gcagaagcca ggaagacatt cgcgccagta cccagacggt tcgggaagtt acgggtagag
    22921 gtggaaacca ccgttgcacc gtccgccaca cgcgcctggt tacccataca cagggaacag
    22981 ccagggatct cgatacgcgc accactctta ccgaagacgc tgtagtagcc ttcttcggtc
    23041 aactgtgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    23101 ttatgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttggt catgcaggaa
    23161 ccgataaaca cttcgtcgat cttctcaccc tgtaccgcag acagcggacg cgcgtcatcc
    23221 gggtcgttcg gagcacacag gattggctct ttaatatccg ccagatcgat gtcgatcact
    23281 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    23341 atgccctgaa tacggcgttc cagggtacga cgatcgccgt aaccttccgc gatcatccac
    23401 ttcagcagga cgatgttgga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    23461 gtacaaccag cggcagaacg ctcggcggac gcatcggtca gctcaaaggc ctgctcaact
    23521 ttcagatccg gcagaccttc gatttccagg atgcggccag agaagatgtt tttcttgcct
    23581 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    23641 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    23701 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    23761 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    23821 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    23881 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    23941 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    24001 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    24061 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    24121 attttttgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    24181 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccaata
    24241 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    24301 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    24361 tcaattacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacctgcg
    24421 tcttccattg tgttaaagaa gattggtgca attttaccgc cgaggcacaa accaccgccg
    24481 cgtttgttcg gcacatgtgg aatatcatca cccataaacc acagtacgga gttcgtggcg
    24541 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    24601 tgttgcagag cttcgatttg cttgatcgga ccaacaacac ctggctggtc tggctcgatg
    24661 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    24721 gcatccggtg ccggagagag gtcatcggtg ttggtttcgc cagtgacttt gaagacggta
    24781 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    24841 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    24901 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    24961 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    25021 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    25081 aggaagcctg ctttgacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    25141 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    25201 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    25261 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    25321 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    25381 cgggcagaat agcaggattt tgacgaggtg ttaatctgtt tacaaaaaag caacataaaa
    25441 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    25501 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    25561 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    25621 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    25681 tttgcagtgc cgggctttgt gccgcctcta ccgggctata tataaaaagc cgcacagtgg
    25741 aagcgcctgt ggaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gcggttacgt
    25801 ttcctgcaac ggtttccgca cctcccgtaa cacccgccgt cgtcaaatcc gcattcagca
    25861 ctgcacaaat agatcaatgg gtcgcgcccg tcgcgctgta tcccgacgcc ctactttcgc
    25921 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    25981 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    26041 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    26101 aatgggtgca aaacctgggc gatgcttttc tggcccagcc gcaggacgtg atggactcgg
    26161 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    26221 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    26281 taccatccaa tacagtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    26341 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    26401 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    26461 aaccgtttgt tgacagcttt gtacgcggat tcggctatag catgggcgtt gctaccacgt
    26521 acgcactatt cagcagcatc gactgggacg acgacgatca tgaccatcat catcatgacg
    26581 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    26641 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    26701 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    26761 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagc gccacgcaat
    26821 tacctgcccc aacgcgcgac agccagcgtc aggcggcagc aaatcagttt cagcaacgaa
    26881 cacacgccgc accagtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    26941 atgaagctga acactatggg agctatgacg acttccacga cttcagccgt cgccaaccac
    27001 tgacccagca acaaaaggac gccgctcgtc agcgttatca gtccgcctcg cctgagcagc
    27061 gccaggcagt tcgcgagaga atgcagacta acccgaagat ccagcagcga agagaggcag
    27121 cgcgtgagcg cattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    27181 agactaaccc gcagaaccag cagcgaagag acgcagcgcg tgagcgtatt cagtccgcat
    27241 caccagagca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aatcaacagc
    27301 aacgtgataa cgcccgccag cgtgttcaat cggcatcacc tgaacaacgt caggtttttc
    27361 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    27421 tgaataacga gcaacggtca gcagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    27481 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    27541 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcggca ggtcggtaat
    27601 gctaccttcg aacacttctg ccgccaggcc cacagactcg tgcagagtcg ggtgcgcgtg
    27661 gatggtcagt gcgatgtctt cagcatcaca acccatttcg attgccaggc cgatttcacc
    27721 cagcagctcg ccgccgttag taccgacaat cgcaccaccg atcacacggt gagattcttt
    27781 gtcgaaaatc agcttggtca taccgtctgc gcagtcggaa gcgatagcac gaccagaagc
    27841 agcccacggg aaggtggcgg tttcatagct gatgcctttc tctttcgctt ctttctcagt
    27901 cagacccacc catgcaactt ctggttcggt ataggcgatg gacgggataa ctttcggatc
    27961 gaagtagtgt ttcttaccgg cgataacttc agcggcaacg tgaccttcgt gaacaccttt
    28021 gtgtgccagc atcggttgac cgacgatatc gccgatagca aagatgtgcg gtacgttggt
    28081 acgcagctgt ttgtcaacgc ggatgaaacc acggtcgtca acttccacgc ctgctttgcc
    28141 tgcgtcgagg tttttaccgt tcggcacacg accaatcgct accagcacgg cgtcgtaacg
    28201 ctgcggttca gcgggtgctt ttttgccttc catcgtcaca taaatgccgt cttctttcgc
    28261 ttcaacggcg gtaactttgg tttccagcat caggttgaat ttcttgctga tacgcttggt
    28321 gaagacttta acgatgtctt tgtcagctgc cgggataacc tggtcgaaca tttcaaccac
    28381 gtcaatctgt gaacccagcg cgtggtaaac ggtgcccatt tccagaccga tgataccgcc
    28441 acccattacc agcaggcgtt ctggtacttc tttcagttcc agcgcgtcag tggagtccca
    28501 gatacgcgga tcttcatgcg gaataaacgg cagttggatc gggcgagaac ccgctgcaat
    28561 gatcgcgttg tcgaagttga tcacggtttt gccgttctca ccttcaactt ccagggtgtt
    28621 agccccggtg aatttaccca gaccgttgac cactttgact ttgcggcctt tcgccatacc
    28681 agccagacca ccggtcagct gattgatcac tttctctttc caggtacgaa tcttgtcgat
    28741 atcggttttc ggttcgccga agacgatacc gtgttcagcc agcgctttgg cttcttcgat
    28801 aacttttgct acgtgcagca gtgctttaga agggatacag ccgacgttca ggcaaacacc
    28861 gccaagggtg ttgtaacgtt ctacgattac ggtttccaga cctaaatcag cgcaacggaa
    28921 ggcagcggag taacctgcgg ggcctgcccc aagtaccacg acctgagttt tgatttcagt
    28981 actcatcatg acctctatat atttatctcc ggcggtcata cccgtcgtct ttcaggccgc
    29041 agcggcgtta gcttcacgcc ctcaccccag tcacttactt atgtaagctc ctggggattc
    29101 tggcgcttgc taccttgctg cacccagaaa tccatagggt atgtgaagta ataccctaac
    29161 caccaccggg tcgttctatc cgtcggtatt ttacaaaatt gttaacaatt tttaaacaac
    29221 aaacggcaac cgatttgtct attcgctaat aacctcaata cattcatgag attaccagaa
    29281 aaaagccggc cgttgggccg gctcttttac ttacatcacc agacggcgaa tgtcagacag
    29341 cgtgttgtta atgatggtaa tgaaacgggc accatcagca ccgtcgatca cgcggtggtc
    29401 gaaggagaga gaaatcggca gcatcagacg cggcacgaac tctttaccat tccacaccgg
    29461 ctccatcgcg gacttggaaa cgccgaggat agccacttcc ggcgcgttca caatcggcgc
    29521 gaagtgggta gtacccaggc cgccgatgct ggagatggtg aagcaaccgc cctgcatttc
    29581 gcccgcagtc agcttaccgt cacgcgcttt cttagaaata gtcatcagct cgcgagacag
    29641 ctcgatgatg cctttcttgt tgacgtcttt gaataccgga acaaccagac cgttcggggt
    29701 atccaccgcc acaccgatgt tgatgtattt cttcagggtc agacgctgac cgtcttccga
    29761 cagcgaactg ttgaagcgag gcatctgctc aagagctgca gcaacggctt tcatgatgaa
    29821 gacaaccggg gttatcttca catccagctt acgtttcgcc gcttcttcgt tctgctgttt
    29881 acggaacgct tccagctcgg tgatatcggt tttgtcgaag tgagtaacat gcgggatcat
    29941 cacccagtta cggctcaggt tcgccccaga gattttctgg atacggccca gttccacttc
    30001 ttcgatttca ccaaacttgc tgaagtccac cttcggccac ggcagcatgc ctgggatacc
    30061 gccgccagtc gccgccggag ctgcttctgc acgtttgata gcttctttca cgtaagcctg
    30121 aacgtcttcg cgcaggatac gacctttacg gccagtgccc ttcactttcg ccaggttaac
    30181 gccaaactcg cgtgccagac ggcggatcag cggagtcgcg tgaacgtaag cgtcgttttc
    30241 agcaaattca gatttgcctt ccgcttttgc agccggagct gctgccgggg cttcagcttt
    30301 tgctgccgga gccggcgctg ccgcttcctg tttcgcagga gctgcagcag gcgctgcgcc
    30361 ttcaacttcg aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt
    30421 cagttccttc acgacgcctg caaacggagc cggaacttcc atagacgctt tatcgccttc
    30481 tacggtgatc agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt
    30541 gacttcaact tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg
    30601 tgcaggagcc gctgccggag ccgcttcctg tttagccgcc ggagctgccg cgcctgcttc
    30661 acccgcgact tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac
    30721 tttgatctct ttcacggtgc cagcaaacgg agccggaact tccatagaag ccttgtcgcc
    30781 ttctacggtg atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc
    30841 ggtcacttca acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgctgcagc
    30901 cgctggtgct gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc
    30961 tgctgcaccg tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc
    31021 aacagagact ttgatctctt taacaatacc cgcctgcgga gacggaactt ccatagaggc
    31081 tttgtcgcct tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac
    31141 caggatctcg gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc
    31201 cattattctt ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat
    31261 ttggcgattg cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc
    31321 agcgccgcta caaccacgta ggaagcatca acttcgaagt ggtgacgcag gttctcacgg
    31381 ctgtcggaac gaccgaagcc atcagtaccc agtacgcggt agtcgtcagc cggtacgtaa
    31441 gtacggacct gctcagcgaa cagtttcata tagtcggtag atgccactgc cggagcgtcg
    31501 ttcatcacct gagcgatata cggaacgcgc ggagtttcca gcgggtgcag catgttccag
    31561 cgttcacaat cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca
    31621 gaacctacgc cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggata
    31681 gaaccggagc ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag
    31741 atacctttac ggataccttc ctcagcacct tccggcattg ccggcatgtg atagttttcg
    31801 ttcagcgtgg tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga
    31861 ccgtcatgca tgatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc
    31921 gggatagtca gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcgccgttc
    31981 agggtggtac gaccggaagt accgccgatc aggaagccac gcgcttgctg gtcgccagcc
    32041 gcccagcaca gatcgccaat acgctggaag ccgaacatcg agtaatagat gtagaacgga
    32101 atcatcggca gattgttggt gctgtaagag gtcgccgctg ccagccagga acaacctgcg
    32161 cccagctcgt tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca
    32221 acctgctcgc ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga
    32281 cggaacagac cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga
    32341 tctttgatcg acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta
    32401 gagatctctt tgctctgctc ttccaacagc gcgccgaagt cttgcaggct cggcagctca
    32461 agcttctcgg tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga
    32521 gcgtgcagat aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg
    32581 atatctgcat cagacaccgg cacattgaaa cggtcgcgga tatgacgcac accgtccatg
    32641 ttcattttct taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa
    32701 cctttaatgg tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg
    32761 aatgcagcgt agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg
    32821 tcagtccagt ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga
    32881 acgtacgcac catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc
    32941 agctggatca gtttaccgct ggtatcctta cgcagcagtt catcccaacg gctaccccac
    33001 atcactttga tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc
    33061 ttgccgttac cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaagacc
    33121 aggttatcca gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc
    33181 atttcaccgt caccgaggaa cgcgtaaacg gtttgtttag aggtatcttt caggccacgg
    33241 tgttccagat atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata
    33301 gatacggtcg ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg
    33361 ccattgccgt gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc
    33421 aggaaagcac gagcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg
    33481 ccatcctgct cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg
    33541 gaagactgga aggacgccat atggccgccc agttcgaggt cttttttcga cgcacgcaga
    33601 accgtcatga tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta
    33661 cccggatact ccggttgttc ttcaacgggg atggtgttga tgtagttgct gataccggtg
    33721 cctgcggcta cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac
    33781 tgagcacgct caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga
    33841 gtttcgatcg gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc
    33901 taataacgtt gagttttctg gaacctgtcc cattgaactc tcgccggaaa gctcaataag
    33961 acaggttcta cgtttagttg ccgcgctttt atatgcgctt gatttacaac atcttctgga
    34021 taatttttac cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc
    34081 acggcggctt tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg
    34141 atgcgatgct tcgcgcgctt cttccggctt accggccata atcgcttcaa atatgcgggt
    34201 gcggtgacta ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg
    34261 gcggacattc tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt
    34321 gtgggccgct tcagtgacag caatctgata ctggagtacg gcgtttgatt ccgcgtccag
    34381 atcgccagac tgctgcgcca gttctatggc gtgatggagt tcacggatgc gttccttgtc
    34441 ttcatcggta ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt
    34501 ttcgagcaag tcatactgtg actcaggatg gtcggagagc agctccacca gcggatcgct
    34561 gaagctttgc cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa
    34621 gcccttcgct tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcaaactg
    34681 ttttgccagt tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat
    34741 caaaaactcc agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta
    34801 ggccatgagt tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt
    34861 ctgtgacgct aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca
    34921 tgaaactgtg cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca
    34981 caaatgtaga ctttacgtag gaaaggagtt ttttaacgat tattaaacta taaaaatccg
    35041 aattgaaccg attcacttac caattttgtg atttttaatt caattaaaac gaatttaaat
    35101 tcattctaca tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa
    35161 ctcatcttca accatgcata aagcgggtgc attcgctgcc gaataccatt attcttgatc
    35221 tgacggaagt ctttttgtaa caattcaaac ttctttgatg taaacaaatt aatacaacaa
    35281 acggaattgc aaacttacac acgcatcact gcgtagatca aaaaaacaac caccgcacga
    35341 ggtttcatga tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc
    35401 catattcagc ttatcgcgct gggtggcgcg atagggaccg ggttattcct gggtagcgcc
    35461 tccgtaatac agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc
    35521 gcctttctga tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc
    35581 tttagccact ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac
    35641 tactgggtac tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt
    35701 cagttctggt atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt
    35761 aacgccatca acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt
    35821 atcaaagtta ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc
    35881 aacggcggcc cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac
    35941 ggcttcaccg gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa
    36001 ctggtgggga tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact
    36061 aaccaggtta tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca
    36121 ctgatgccgt ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag
    36181 ttaggcgata cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc
    36241 gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggt
    36301 aatgcgccaa aagcgctggc gtctgtcgat aaacgtggtg taccagtaaa taccattctg
    36361 gtgtctgcac tggtaacggc gttgtgcgta ctgattaact accttgcccc agagtccgct
    36421 ttcggactgt taatggcgct ggtggtatct gcactggtaa tcaactgggc gatgattagc
    36481 ctggcgcata tgaaattccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct
    36541 gctctgcttt atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg
    36601 attatgctga tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc
    36661 gtgttaggta tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa
    36721 tctctctacg ccctcacccg tacagggtga gggcaataat ctttacacca acgccccgta
    36781 aatcgtcagt agtgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac
    36841 cgctgccttc ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc
    36901 cagacgcgtt aacacctgat actgcgaagt atggaaatca cccagcgaag caaaccaagc
    36961 cggtaacgct ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg
    37021 cacccaatcc agtacatgaa gcacggcatc aatgccggac tgtaaacgat gatgcggcgt
    37081 ctgatatcgc gccagccagt attgccatgc acgcaaaaac gcatacccca tcagcgtaac
    37141 gggtccccag gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt
    37201 aatccacagc aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg
    37261 gacgccgtga atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg
    37321 tgaagcagct gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag
    37381 caaaccaatc agcagccaca ccagtagcgt gggaacgttg aacaatactc cctgcaatgc
    37441 gcgtaacagt aaaaaagtca cgcccatcgc aataatggtc atgcctaacg tgcgcccgag
    37501 agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc
    37561 gcccaactta aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt
    37621 gtctccttgc tgaccagcac acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt
    37681 ttccgatccg gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga
    37741 tctatcagtg cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccagc
    37801 gtatcggtgc cttcaagctc aatcccaata gaaaaatcat tgcagcgttc gcgcccctga
    37861 tactgagaga ctcccgcatg ccatgcacgt ttatcgaaag gaacatactg gactatttca
    37921 ccatcacggc gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa
    37981 ggatgtgcct gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac
    38041 tcgcctggcg gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccggg
    38101 cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac
    38161 aacatgcagg atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac
    38221 cttatgattc gttagctatc tggagtttta acatgccgcc tcgccgctat aaccctgaca
    38281 cccgacgtga cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg
    38341 cgctgcggga agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt
    38401 taccggaaaa ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgcg
    38461 gcaaacgctg ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct
    38521 ggcatgtgga tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc
    38581 catcccgcgt gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag
    38641 gagttgccag taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt
    38701 tggatacgcg caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg
    38761 gcggcggagc gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaatcata
    38821 ttattgcctc cggctcagtg cgccaggccg tcgaaaaagc gtcctggctg cacccggatg
    38881 cgccagtaga agtcgaagta gagaatctgg aagaacttga tgaagccctg aaagcaggag
    38941 ccgatatcat catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca
    39001 ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat
    39061 ttgccgaaac gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac
    39121 tcgacctttc aatgcgtttt cgctaattgc tggcagattt atccgctcca tcgcgggcgg
    39181 ataatctccc cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc
    39241 aacgaaataa aaaacctcgg aagctgcttt cccgtttcgt tttttgcccc tcgcttgtgc
    39301 cgatggcgtt tgccaaagta gcgccaacca aatcaaggag cgaaacagat ggacaagcaa
    39361 cgcggtttta cacttatcga actgatggtg gttattggca tcattgccat tttaagcgcc
    39421 attggtattc ccgcttatca aaactacctg cgcaaagctg cactcaccga catgctacaa
    39481 acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat
    39541 acctgcgacg gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc
    39601 atgagtgtgg caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc
    39661 gtcgtcatga cgctaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc
    39721 aatatccaaa gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac
    39781 gccaactaag gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag
    39841 ggagtcttgc tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca
    39901 catgagttgc tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg
    39961 acgcgccaac aaatggaagg tcacgccagt cgcacacaac agacattgcc cgtagctgtt
    40021 caggagaagc atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa
    40081 caacgcgcgt ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt
    40141 atcgacggcg tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaacc
    40201 gccagattaa aagtgctgag aaacctggat attgcggaac atcgcctgcc gcaggacggg
    40261 caattcactg tcgaactggc aggaaacgcc gtctcatttc gtattgcgac cttaccatgt
    40321 cggggtggtg aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtc
    40381 aacacgcttg gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca
    40441 cagggactgg tgctggtaac tggccctaca ggcagcggca aaacggtcac gctttatagt
    40501 gccctgcaaa cgctgaatac cgctgacatt aatatttgta gcgtcgaaga tccggttgag
    40561 atccccatag ccggactaaa ccagacgcaa atccatccgc gtgccggact cacctttcag
    40621 ggcgtgttgc gtgcgttatt gcgccaggat cctgacgtca tcatgatcgg agagatccgc
    40681 gatggcgaaa cagcagagat cgctattaaa gcggcgcaaa ctggtcacct ggtgttgtct
    40741 accctacaca ctaattccac ctgcgaaacg ctggtacgtt tacagcaaat gggggtcgcc
    40801 cgctggatgc tatcatcggc gcttacgctg gtaatagccc agcgtctggt acgcaaactt
    40861 tgcccacatt gtcgccggca gcaaggggag cccatccaca ttccagacaa tgtatggcca
    40921 tcgccgctgc cccactggca ggcacccggt tgtatacatt gctaccacgg tttttatggt
    40981 cgtacggcct tatttgaagt tctgcccata acgccggtca ttcgtcagct tatttccgct
    41041 aataccgacg ttgaatcgct ggaaacgcac gcacgacagg cgggtatgcg tacgcttttt
    41101 gaaaacggct gcctggccgt agagcaaggc ttaaccacct ttgaagagtt aatccgcgta
    41161 ctggggatgc cgcatggcga gtaagcaact ctggcgctgg catggcataa ccggcgacgg
    41221 caatgcgcaa gatgggatgc tatgggcaga gagccgtact ttactgctta tggcactaca
    41281 gcaacagatg gttaccccac taagcctgaa gcgaatcgcc atcaattctg cgcagtggcg
    41341 aggagataaa agcgcggaag tcattcatca actggcgacg ctactcaaag ccgggttaac
    41401 gctttctgaa gggctggcac tgctggcgga acagcatccc agtaagcaat ggcaagcgtt
    41461 gctgcaatcg ctggcgcacg atctcgaaca gggcattgcc ttttccaatg ccttattacc
    41521 ctggtcagag gtatttccgc cgctctatca ggcgatgatc cgcacgggtg aactgaccgg
    41581 taagctggat gaatgctgct ttgaactggc gcatcagcaa aaagcccagc gtcagttgac
    41641 cgacaaagtg aaatcagcgt tgcgttatcc catcatcatt ttagcgatgg caatcatggt
    41701 ggttgtggca atgctgcatt ttgttctgcc ggagtttgcc gctatctata agaccttcaa
    41761 caccccacta ccggcactaa cgcaggggat catgacgctg gcagacttta gtggcgaatg
    41821 gggctggctg ctggtgttgt tcggctttct gctggcgata gccaataagt tgctgatgcg
    41881 ccgcccgacc tggcttatcg cgcggcagaa attgctgtta cgcatcccga ttatgggttc
    41941 actgatgagg ggacaaaaac tcacgcagat ctttacgatt ctggcgctga cacaaagtgc
    42001 aggcattact tttttacagg gcgtagagag cgtcagagaa acaatgcgct gcccgtactg
    42061 gatgcaactt ctgacacaaa tccagcacga tatcagtaac ggtcatccca tctggctggc
    42121 gctaaaaaat accggtgagt ttagcccgct ctgtttgcaa ttagtgagaa caggagaggc
    42181 atccggctcg ctggacctca tgttagacaa cctcgcccat catcatcggg ataacacaat
    42241 ggcgctggcg gataacctcg cagccttact ggaaccggcg ttgctgatca taacgggagg
    42301 aattatcggt acgctggtgg tggcaatgta tctgccaatt ttccatttag gcgatgcgat
    42361 gagtgggatg ggataacgct ggcgtgttgc tccacgccag cgttgggaga ttaaaggttg
    42421 ttgaaaatgc ggttttcttg ttccagcaca cgaataaacg tggtgcgctt ggtcagctct
    42481 ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca ggccgcccaa aatatcacgc
    42541 gcggtatttt caaccgggcc tcgcagcggc agcttaacgg ttttaccttc tgctgcgcga
    42601 tattccgcaa cgccgccaac gtgacgtttc atcgcagact cggagctcat gccgtagaac
    42661 agcataaatt tctcgccgtt ctcctcaacg atgcgaccgc cgctctcttc gtggcccgcc
    42721 agcatgccgc caagcatgac gaaatcggca ccgccgccaa aggctttcgc cacatcgccc
    42781 ggcgtggtgc agccaccatc gctgacgatc attccgccca gaccgtgcgc agcatcggca
    42841 cattcgatta ccgcagaaag ttgcggataa ccgacgcctg ttttgacgcg agttgtacaa
    42901 acagaacctg ggccaatgcc aactttaacg atatcggcac ctgagaggat aagctcctca
    42961 cacatttcac cagtcactac gttaccagca caaatggttt tggtcggcca cgcttcacgc
    43021 gctttcgcaa cgaactgcac gaagtgttcg gaataaccat tcgccacgtc aatacaaacg
    43081 aagtttaatg ccgggttcag gtcgagaatc tgtttagttt tttcgaaatc cgcatcagac
    43141 gtaccggtag aaaccatcac atgtttcagc acatcagcgg aagaattgtt gataaacgct
    43201 tgccactctt cgacagaata gtgtttatgc acagcagtca aaatatcaaa agaagccagc
    43261 gcagaggcca tagaaaatgt gcctacggtg tccatatttg cggcgataat cggcacgccg
    43321 gaccagctct gacctgaatg tttgaaggtg aattgacgtt ccagttcaac atcggaacgg
    43381 cttttaagag tggagcgttt agggcggatg agaacgtctt taaaacctaa cttcagatct
    43441 tcttcaatac gcatgtgcgg attcctgggg ttaatggcta taagggtaag agcgcaactc
    43501 cagtgacgct atcatacgca ctaatcaatg cggcgcaaga ctgcgaaatt gtcttttttt
    43561 acgctacaat cccataaatt tacctggtaa atagtgagcg aacgtttcat caatttcgct
    43621 ttcgtgcact catttttaac gttttgataa tcatgatgaa ttcccgggaa tataaattat
    43681 gaggtatata gttgccttaa cgggaggcat tggcagtggc aagagtaccg ttgccaatgc
    43741 gtttgctgat ctcggaatta acgtcattga tgccgatatt attgcgcgtc aggtggttga
    43801 accaggtgca cctgcgctac atgccattgc tgatcacttt ggcgctaaca tgattgctgc
    43861 tgatggaaca ttgcagcgcc gggccttgcg cgagcggatc ttcgccaacc cggaagagaa
    43921 aaactggctt aacgccctgc tgcatccgct gattcagcaa gagacgcaac accagatcca
    43981 gcaagctact tccccctatg tactgtgggt tgtgccattg ctggtagaaa actcactgta
    44041 taaaaaagcg aatcgagtgc tggtggtgga tgtcagccca gaaacgcaac ttaagcgcac
    44101 catgcagcgc gatgatgtaa ctcgcgagca tgtcgaacaa atccttgctg ctcaggcaac
    44161 gcgcgaagcc cgccttgccg tggcagatga cgtcattgat aataacggcg caccggatgc
    44221 tatcgcatcg gatgttgccc gcctgcacgc acactatttg cagcttgcgt cgcagtttgt
    44281 ctcacaggaa aaaccgtaat gcagacccag gtcctttttg aacatccact aaatgaaaaa
    44341 atgcgtacat ggctgcgcat tgagtttttg attcagcaac tcaccgttaa tttacccatc
    44401 gttgaccacg ctggcgcgct gcatttcttc cgtaatgtca gtgaattact ggatgttttc
    44461 gagcgcggcg aagtccgcac tgagctgttg aaagaacttg accggcagca acgtaaactc
    44521 cagacctgga ttggcgtgcc tggcgtggac cagagccgta ttgaagcatt aattcagcag
    44581 ttaaaagcgg cggggagcgt attaatttcc gcgccgcgta tcgggcaatt tctgcgtgaa
    44641 gatcgtttga ttgctctggt gcgtcagcga ctgagcatcc caggcggctg ttgcagcttt
    44701 gatttaccta cattgcacat ttggctgcat ctaccccagg cgcagcgcga cagccaggta
    44761 gaaacctgga ttgccagcct gaacccgctc acccaggcac ttaccatggt gctggattta
    44821 attcgccagt cggccccctt ccgtaaacaa accagcctga atggttttta tcaggataac
    44881 ggtggcgatg ccgacttgct gcgcctgaat ctgtcgctcg attcacagct ttatccgcaa
    44941 atttccggac ataagagccg ttttgccatt cgttttatgc cgctggacac tgaaaacgga
    45001 caggtaccgg aacgtctgga tttcgaactg gcctgttgct aaggagtaaa aatgtcagaa
    45061 actattacgg tgaattgccc aacctgcggg aaaacggtgg tgtggggtga aatcagcccg
    45121 tttcggccat tttgctccaa acgttgtcag ctgatcgacc tcggagaatg ggctgctgaa
    45181 gaaaaacgaa tccccagcag tggcgatctt tccgaaagcg atgactggag cgaagagcca
    45241 aagcagtgac atttgtctga tgccgcacgt aggcctgata agacgcggac agcgtcgcat
    45301 caggcatctt gtgcgaatgt cggatgcggc gaaaacgcct tatctgacct acagacgttt
    45361 aagcttcgca attaccggtt cattggctgg cggaaaatca tcggcattaa gatcgaccag
    45421 cgccatccac tcaccgggtt gcccttcttt accccacggc ttcccttccc agcgttcgac
    45481 cagccagaac cacagtgtta tatgcctgtc cgggaattca tattccagtt tttcaaatag
    45541 cgaaaaatgt tgtggggtaa tcccgacttc ttcctgaagt tcacgcacca ccgcctgttc
    45601 cggcgtttca cccatttcaa ttttaccgcc gggaaactcc agtttattcg ccatgtgcgc
    45661 atctgctgcg cgacgcgtta taaagatttc attgttctcg ttgcgaataa tacctaccgc
    45721 aatttgcagc tttttcattg tcttaaacct ataaaaaagg cgcagaatcc tgcgcctttt
    45781 acttcaactg ttagctttta ttgcaggcga ccatggcact gcttgtattt tttaccagaa
    45841 ccgcacgggc aaggatcgtt acgtcctact ttgcgttcac cggtttgcgc cgccagtgca
    45901 gctgcggctg cagagtcgtc atcctgatgg ctaagctgct gcatttgcgc taaacgctcg
    45961 gcttccatac gacgctgttg ttccagctcc tcaacctctt caggcatacg tacctgaact
    46021 ttgctcagcg tactgataac ttcatatttc aacgactcca gcatcgctgc aaacatggag
    46081 aacgattcac gtttgtattc ctgcttcgga tctttctgtg catagcctcg caggtggata
    46141 ccctgacgca gatagtccat cgctgccagg tgctctttcc acagggagtc aagcgtttgc
    46201 agcatgacgc ctttctcgaa gtgacgcatc atctctgcac caaccacttc ttctttacgc
    46261 tgatacactt cgatggactg cgccagaatg cgctcacgca gcgtctcttc atgcagttcg
    46321 ggttctttat ccagccactc agcaattggt aaatcgaggt cgaaatcgtt cttcagacgt
    46381 tcctgcagcc ccggaatatc ccacatttct tccagcgact gcggcggaat gtaggcatca
    46441 atggtggctt tgaacacatc ttcacgaatg ctgttgatgg tttcgctcac atcgctgaca
    46501 tccagcagtt cgttacgctg ggagtaaatg gcgcgacgct gatcgttagc cacgtcatca
    46561 tattccagca gttgcttacg aatgtcgaag ttacgacttt caactttacg ctgggcgttg
    46621 gcaatcgctt tggtcaccca cgggtgttca atggcttcgc ctggcttcat acccagttta
    46681 cgcatcatgc cggatactcg gtcggaagca aaaatacgca tcagcgcatc ttccatcgac
    46741 aggtagaaac gggaagaacc agcatccccc tgacgaccag aacgaccgcg caactggtta
    46801 tcgatacgac gggattcgtg acgctcggta ccgatgatat gcaggccacc tgctgccagt
    46861 accgcatcgt gacgtacctg ccagtcggct ttaatttttt caatttgctc tgcggtcgga
    46921 ttttccagcg cggcaacttc tgcctgccag ctaccaccga gcacaatatc tgtaccacga
    46981 cccgccatat tggtcgcgat agtcaccgca gccggataac ctgcctgagc aacaatcgcc
    47041 gcttcgttgg cgtggaattt ggcgttcagg acgttgtgct taataccggc tttggtcagt
    47101 tcgtttgaca ccagctccga tttttcgatg gagatagtac ccaccagcac cggctggcct
    47161 ttcgcagtac gttctttgat atcttcaatg atcgcctgaa ttttttccgc ttcagtcatg
    47221 tagaccaggt ccggcagatc tttacgaatc attggacggt tggtcggaac aacgacggta
    47281 tccagcttgt agattgagct aaattcgaaa gcttcggtat cagcagtacc ggtcatcccc
    47341 gccagttttt catacagacg gaagtagttc tggaaggtga tcgaagccag cgtttggttt
    47401 tcgttctgga tctgcacacc ttctttcgct tccacagcct ggtgcagacc atcggaccag
    47461 cgacggccct gcatggtacg accggtgtgt tcgtcaacga tgataacttc accatcttta
    47521 acgatgtagt cgacgtcacg ggtaaacagc gcatgagcgc gcagcgccgc cgttacgtgg
    47581 tgcatcagca tgatgttggc cggagagtac agagactccc cttcatccat gatgccctct
    47641 ttcaccagca gttcttcaat cagcaccaga ccacgttcgg tcaggttcac ctggcgagat
    47701 ttttcgtcca ccgagaagtg gccttcgccc tggaaggttt cggagtcttc tttttcctga
    47761 cggatcaggt gcggaataat tttattcacg cgtttataca tttccgagct gtcttctgcc
    47821 gggccggaaa tgatcagcgg tgtacgcgct tcatcgatca ggatggagtc cacttcgtcc
    47881 accagcgcat agtgcagttt acgctgtaca cgttcttcag ggctgaacgc catgttgtcg
    47941 cgcaggtagt caaagccgta ttcgttgttc gtaccgtaag tgatgtcagc tgcgtaagct
    48001 tcgcgctttg ccggtgctgg catgcccggc aggttgatac cgacagtcag gccaaggaat
    48061 tcaaacagcg gacggttgtt ttcggcgtca cgttgcgcca ggtagtcgtt gacggtaact
    48121 acgtgcacgc ctttaccggt tagtgcgttc aggtaagcag gcagcgttgc ggtcagggtt
    48181 tttccttcac cggtacgcat ttcggcgatg cagcgttcgt taagaaccat accgccgagt
    48241 aactgaacgt cgaagtgacg cataccaaag acgcgcttac ttgcctcacg taccacggcg
    48301 aaagcttccg ggatcagatt ttccagcact tcgccttttt ccagacgtgc acgaaactct
    48361 gcggttttcc ctttcagttc ttcgtcggag agtttttcca tctccggttc catggcattg
    48421 atgatgttga ccactttgcg catccggcgc agggtgcgat cgttacgact accgaaaact
    48481 ttagttaaca atttgattag cataataaaa tctcaaacgc cccgcgttgc ggagttaata
    48541 aaatgaagta aaggtttatt gttgttaggt gaggcgttga gggccagcac ggatgccttg
    48601 cgcctggctt atccagacgg gcgtgctgaa ttttgcttgt ggggtaaaat gcgcataatc
    48661 aatgcgataa cccttttcag acggcgtgcc ttcctgggtc agcagcgcgc tgagcgtatc
    48721 cagtaatgca agatgttgcg cctgaagagg caaagattct tcagcaacgg gcagtgtttg
    48781 cggtgccatt gcgaaagaaa gatgacggat taccgtgcga atggcatgtt gatgccagta
    48841 atcaacggaa tagttcgaat tcgggcggcg tgtgttcgct tccagcaagg ccaattgacc
    48901 aaagttaact ttggctgaag gctcgtggtt gcgggttgtc gcttttgcgg gcgcgtttgg
    48961 ttcggcggcg ttgctgagcg caggcaaacc taaactcgcc gcaaccatcc ctaataagag
    49021 atgcggccag aagtagcgtt taccaaactg tcgccagcgc gtcagtattc cactcacgtt
    49081 attgccatcc cgatcgtatt caaacgtatt tagcgcacaa atcttatcat taaagcctgc
    49141 agtccacagc acgaatgaag ataagttgtg cggaaaagtg cttaagaaaa cagcgttcgc
    49201 accgtttttt atagctctta taaaattcaa aaaaaaacga ctggttcacc tggccggaga
    49261 gagtgccaga tttgccagtc gaattttata cgacagtata aatgtcgtta tgccagtaca
    49321 gctgaaggcg ctttgaaggc caacggcagt tctgcgtcgt cctggaaggt cacatattcc
    49381 caggcttcct gtttcgccag gacagcctgc agcagtttgt tattcagtgc atgaccggat
    49441 ttataagcgg taaatgcacc aataatattg tgaccacaca tgaacaagtc accgatcgca
    49501 tcgagcattt tgtgacgcac aaattcgtct tcaaaacgca ggccgtcttc gttcagtacg
    49561 cgataatcgt caacaacgat ggcacaatcg aagctgccgc ccaggcacaa accacgggac
    49621 tgcagatatt cgatatcacg catgaaaccg aacgtacgcg cacggctgat ctggcgcata
    49681 aacgcatcag cggagaagtt catcgcatag cgctggttgc tggaatcaat agccggatgg
    49741 ttaaaatcga tggtgaaatc cagcgaaaaa ccattgtacg gcttaaattc agcccactta
    49801 tcgccatctt cgacacgaac agtctctttg atgcgaacaa attttttggc gcagttcaac
    49861 tcgtcgatac cggcgtcaag cagcaggtat acaaacggag cggcgctgcc gtccatgatc
    49921 gggatttccg gcgcgttaac ttcgataaca atgttatcga tgcccaggcc cgcgagagca
    49981 gcattgaggt gctctacggt tgaaatccgt acatcatgct cgttgaccag acacgtacag
    50041 agcatggtat cacgcacaga tttggcatcg gccgggaaat ctaccggtgg attcaagtcg
    50101 gtgcgacgat agatgacccc ggtgttggcc ggcgcagggc gtaacgtcag ggtgactttc
    50161 ttgccggtat gtaaaccgac acccgtcgcc tgaacgatac gtttaagtgt cctttgtttg
    50221 atcatcgtat tatctcgcca aattacctat ccaaccgaag tgtactatac attcggcggg
    50281 ccagtttagc acaaagagcc tcgaaaccca aattccagtc aattcttaat cagcttgctt
    50341 acgcaggaat gctgggatat ccagataatc cggctctttc gcagtttgcg gcgcattgtc
    50401 attcacgact ttagcaaccg gcttctgctc ctgggtcagc ggagccatcc catgctgctg
    50461 gtagcgatcc atcactggct gctgaacctg cttattggtc accagagtga tttcaggacg
    50521 tttgtccatg ccgatacctg tcgcaacaac ggttacgcgc agctcgtcat tcatatccgg
    50581 gtcaagagaa gtaccgataa ccacagtcgc gttgtcggaa gcaaatgcac ggatggtgtt
    50641 acctaccgtt tcgaactcat ccagacgcag gtcgaagccc gccgtgatgt taaccagcac
    50701 gccgcgcgcg ccagacaggt cgatatcttc cagcagcgga gaagagatag ccatttcagc
    50761 agcttcttcc gcacggtctt caccgctcgc cacgccagaa cccatcattg cgtagcccat
    50821 ttcagacatt acggtgcgta cgtctgcaaa gtccacgttc atcaaacccg gacgagtaat
    50881 cagttcggcg atgccctgaa cagcgccttt cagtacatcg ttcgctgcgc caaacgcatc
    50941 cagcagggag ataccgcggc ccagaacttt cagcagtttg tcgttcggga tagtgatcag
    51001 agagtccaca tgcttggaca gttcagtgat cccctgctcc gcgaatgcca tacgcttctt
    51061 gccttcaaag ttgaaaggct tagtgacgac agcaacagtc aggataccca aatcttttgc
    51121 cacttcagcg acgactggtg ctgcacctgt accggtacca ccccccatac ccgcagcaat
    51181 aaagaccatg tctgcacctt ccagcgccgc acgcaatgca tcgcgatcct catcagccgc
    51241 attgcggcca acttctggat tagcgccagc gcccagtcct ttggtgatac cgctaccgat
    51301 ttgaatcgtc tgtccaaccg ctgttttacg cagcgcttgt gcatcggtat ttaccgcgaa
    51361 gaattcaaca ccttcaatgc gctcgcgcac catgtgttca acagcattac cgccgccgcc
    51421 gccgacgccg atgactttaa tcaccgcgtc attggtaagt tccattggtt caaacatagt
    51481 ttctctccga tttgtgcctg tcgcctgagg ccgtaatcat cgtcggcctc ataaaaatta
    51541 aaactctttt cgcagccaac tattgagtcg cttgatccac gagccaactg atgctgtaac
    51601 acgtttttct acttcagctt caccgttaag atgtgactct ttcccatagt gaagcaatcc
    51661 caccgccgtc gaataatacg gctcctgagc ataatccgtt aaaccggtaa tgttcagcgg
    51721 cgcgccgata cgcacttgcg tatgaaacac gcgctgagca caggctgcaa gaccttcgat
    51781 ctgcgctgcg ccaccggtta atacaatgcc tgccgccagg tgatgtttaa ccccttgttg
    51841 gcgaagcttt tcctgcaact gcaatatctc ttcgttgacc aggttgagca gctcggtata
    51901 gcgcggctcg atcacctctg ccagggtctg acgttgcaga ctccgcggcg gacgaccacc
    51961 tacgctcggc acttccacgc tctcatcttt tccaacgatg gaacccagcg cacaaccgtg
    52021 gcgaacttta atcgcttcgg cgtcgcttgg cggcgtgcca aaggcgtaag cgatatcact
    52081 ggtcacgaca ttgccagcat aaggaattac cttagtgtgg cgcaatgccc caccggtata
    52141 aacggcgata tccattgtac caccaccgat atcgacgacg cagacaccca gttcacgttc
    52201 atcttccgtc aataccgaat aacttgatgc cagaccggca aatatcagtt ggtcaacttt
    52261 cagcccacaa cgttcaaccg ctttgacgat gtttttcgcc atatcgttgt gacatgtgat
    52321 caggtgcact tttgcctgca tccgcacgcc cgaaagtcct accggattct tgatcccttc
    52381 ctgatagtca atcgcatact cttgcgggat cacatgcagt acacgatgct catcgcgcac
    52441 acgcaccgat ttcgcggtat ggacgacgtt ttccacatct tcttgcgtca cttcttcttc
    52501 agaaataggc accataccaa tttcattctg gcagctgatg tgcttaccag aaagcgccag
    52561 atataccgaa gagatctgac aatctgccat caattctgcc tggtcaatgg cgcgttgtac
    52621 gcacttgacc acggattcga ggtcgttcac cccgccttta tccataccac gcgacgggca
    52681 gctgcccacg ccaatgatat tgaccatacc gtcgggcaga acttccccta ctaaagcggc
    52741 aaccttcgcg gtaccaatct ccagtcctac taccagtttt ctgtccgtcg ccttgatcat
    52801 tgttgttctg cctgtgcctg attttgttgc tgagtagatt cctctggcgg caagggcgcc
    52861 cagcctactg ccgctccaga gtcataacgc aaatcaacgt agctaatccg tttgccatcg
    52921 gtttgcgcct gctgctgtaa aaccggataa agttctacaa agcgagccaa acgtttcatc
    52981 gtatcgcccc ggccaagatt gagcttaata tcgttattca gcgtcaactg ccaggaacgc
    53041 cgcgcggtca tcgccgcttc cttcagagta aatctgtcct ttgccagcat ctgccccatt
    53101 tcgcgatagc cctgcaacac ttcattggcg ctgccttccg ggccatacag cattggaagc
    53161 acctgcttgc tggtgcgatc tggcggcacg ctgaaggtgt ttccttccgc gtctaccatg
    53221 tgttgatcat tccaccgcgc aatcggcaca tattcaacca gatgaatctt caattcatca
    53281 ggccactgct ttctgacgct cacctgctta atccacggca ggcgttgttc tatttgcgtc
    53341 tggatgatgt tgacatcctg ggtcataaag gtacccggct cacccaatgc caggatcgac
    53401 tgccggatat cgtcattacg cgtgtaatgg cgttcaccgg tcaacaccag ctttgagagc
    53461 ggcaggcgtt gcgcatcttc catccagccc aacacgaccc agccgctcac caacactgtc
    53521 gttaaaacgg tcagcaggaa aaggatcccc gccagacgcg ttccattatt gcggcgagaa
    53581 gaaacctctt cttcgctgtt tcgcgtgttc agagcagcct gcgacatatt agtccgccag
    53641 ttccagaatt cgtactacca actgcgagaa gctcatacct gcctgacgtg ccgccatcgg
    53701 caccaggctg tggctggtca tacccggtga ggtattggct tccagcagat aaaactgtcc
    53761 atcgctgtcc agcataacgt caatacgtcc ccatcctttg caacctaacg tcgtccatgc
    53821 tttcagcact aatgcctgca aattggcctc ttgtgacgct tccagacctg cggggcagaa
    53881 atactgtgtc tcatcagaga gatacttcgc ctcatactca tagaaggttc cggccggttg
    53941 aatacgtatt gacggtaaaa tttcttcacc gagtatcgca accgtgaact ccggcccact
    54001 tagccatttt tcaatcaata cttcttcatc gtgctgaaat gccaatctta atgcatcttg
    54061 tagagcattt tctgctacta cttttgacat tcccacactg gaaccttcgc ggctcggctt
    54121 aacgataacc ggcaaaccca gagcagaaat ttctgctaac tgcttatcgc tcaggccttt
    54181 ttcaaactct gcgcgggtta acgctaccca cggcgcgacc ggtaaaccgg caccttgcca
    54241 tagaagtttg ctgcgtagtt tatccattga aagcgcagat gccatcactc cgcttccggt
    54301 ataaggcaag cccatcagct cgagcatccc ctgcagcgta ccatcttcac cgccgcgacc
    54361 gtgtagcgcg ataaacactt tctgaaagcc catcgacttc agttgcgtca cgtcgacttc
    54421 tttcgggtcg acaggatacg cgtcaatacc gccttcacgc agtccggcta acaccgctgc
    54481 gccagaattc agagaaactt cccgctcagc ggaggtccca cccaacagga ccgcgatttt
    54541 atcagtcatg ttgttcttcc tccggagttt gcggcttcag tttgatttca gctaaagaac
    54601 gggcaatttt tccaatatta ccagccccct gaacgagaat caggtcgtta ccggttaata
    54661 ccggtgccag catctcggct acccgcgccg gatccggcac cagaatggga tcaattttcc
    54721 cacgtccacg aattgtgcga cacagcgaac ggctgtccgc tcccggaatt ggcgcttcgc
    54781 cagccggata cacttccagc atcaacaggg tatcaacctg cgtcagcaca ttggcgaaat
    54841 catcatacag gtcgcgcgta cgggtaaaac ggtgcggctg aaacagcatt accaggtttt
    54901 tatccggcca gcctgcgcgc gccgctttaa tggtggcgtc cacttccgtc gggtggtggc
    54961 cgtagtcatc gaccagcatt gccgtaccgc ttttaccatt cactggctcc agcgggaatt
    55021 caccgaggaa atcaaaacgg cgaccagtcc cctggaagct ttcaagcgcc cgcaaaatag
    55081 cctcgtcgtc aatgccctct tccgtagcaa ccgcaaccgc agctgcggcg ttcagcgcgt
    55141 tatgacgacc tggcgcattc agggtgacgc gcatcggctc tttgtcctgg cgcagcagcg
    55201 taaagtgccc ctgcgggcca atctgctgat aatcttctac acgcacgtcg gcatcttcgc
    55261 tgaagccgta agtcgtggtc tgacgcccca ctcgcggtaa caattcgcgg atcaccggat
    55321 catcaacaca catcaccgca cgaccgtaaa acggcaggtt gtgcagaaaa ttaataaaag
    55381 tctgttttaa attctcaaag tcgccctggt aggtatccat gtggtcggct tcgatattgg
    55441 tgacaatcgc caccatcggt tgcagatgca ggaacgatgc atcactctca tctgcttcgg
    55501 caatcaggta ccgaccatgc cccaaacgcg catgaacccc cgccgctttt accagcccgc
    55561 cgttaacgaa ggttgggtcg agccccgctt ctgcgtagat gctggaaacc atcgcggtgg
    55621 ttgtcgtttt gccgtgcgtt ccggcaatgg cgatgccatg acgaaaacgc attaactcag
    55681 ccagcatttc ggcacgacgg atcaccggaa tacgcgcttc atgagcggcg acaatttccg
    55741 ggttatcggc agaaatcgcg ctggaaacaa cgaccacgct ggcatcacgt acgttttccg
    55801 ggcgatggtt gaaataaatc gtcgcaccca gattcattaa ctgctgcgtg accggatttg
    55861 gcgctaaatc ggaaccactg atctgataac cttcattggc cagaacttcg gcaataccgc
    55921 ccataccggc accaccaatg ccgacaaaat gtatgtgccg aacgcgacgc atttcgggca
    55981 cgatggaacg cagttttgcc aattgttgtg tattcattct ttacgccatt aacttcttaa
    56041 attcatacga tgcaaaaggc atcgctacaa ttacgcccgg gcaacccggc tcacttcatt
    56101 tgccactcgc tcggtggcat ccggaatgga tgcagcgcgg gcgcgttctg ccatggttaa
    56161 taaggtttct cgcgaccacc cggccagggt gttggcgaca gcatccacgc taagctgtgg
    56221 ctgctcgata attttggctg cgcccgcttt ttccagcggt agcgcattcc agtattgctg
    56281 gcggtcttta tgttgaaacg gcacaaacaa cgccggtagt cctgccgcgg cgatttcact
    56341 caccgttaac gcaccggagc ggcaaacgac gacatccgcc cacgcatacg ccgccgccat
    56401 atcatcaata aattcagtca ctttatgctg cggttgcccc gcttcggcat acgcctgttc
    56461 aacggattgt tgcgaacctt tgccgctctg atgccagata gtgactgaat cacccagttt
    56521 cgcagcaacc tgcggcattg tctggttaag aatgcgtgcg ccctgggaac cacccactac
    56581 caacacacga accggacctt cacgtccagc caaacgttgc tgcggcaacg gcagcgccag
    56641 cacatcagta cgcaccgggt tacccactac ttccgcatta gggaaagcac ctggaaacgc
    56701 ctgcatcact ttggtggcaa tctttgccag ccatttattg gttaagcccg caataccgtt
    56761 ttgttcatga agtacaaccg gaatgcctaa cgaccacgcg gccagaccac caggacctga
    56821 tacgtagccg cccataccga gcaccacgtc aggtttgtac gctttcataa tcgcccgcgc
    56881 ctgacgccag gcgttgaaga tacgcagcgg ggcagctatc agtgctttta taccttttcc
    56941 acgcagacca gagatacgaa tgaaatcaat ttcgatgcca tgttttggca ctaagtccgc
    57001 ttccatacgg tcggcagtcc ccagccagcg aacttgccaa ccctgagcca ttagatggtg
    57061 cgcaaccgcc agtcccggga atacatgtcc accggttccg cctgccatca ccattaatcg
    57121 ctttccttga ccactcatcg tgaacctcgt acaaacgcct gcgctttctc cagacgcgtt
    57181 tcataatcaa tacgcaacag catcatgatg gctgtcgaca taatcagtaa gctcgaacca
    57241 ccgtaactga tcagcggcaa tgtcagacct ttggtcggta acatccccgc cgccgcgcct
    57301 acgttaacca gcgcctggaa gctaaaccag atgccaatag aacaggcgag aaaaccggaa
    57361 aaacggtggt caatttctaa tgctttacgg ccaatcgaca tcgcgcgaaa agcgacgaag
    57421 aataccatta aaagtgccag caccacaccg acatacccca gttcttcgcc gataatggcg
    57481 aaaataaagt cagtgtgcgc ttccggcaga tactccagtt tttgtaccga gttacctaaa
    57541 ccttgccccc aaagttcgcc gcgaccaaac gccatcagcg attgcgttaa ctgatagccg
    57601 ctgccaaagg gatcttccca cgggttccag aatgcggtaa cacggcggat acggtacggt
    57661 tcggcgagta tcagcaacac aaccgctgaa atgcccatac cgataatggc aatgaactgc
    57721 cacaatttcg ctcccgccag gaacaacatc gccagcgtag tcacaaacaa caccaccacc
    57781 gtaccaaggt ctggctgtgc cagcagtaac actgccaaca ccagaatcac gcccatcggt
    57841 ttcaggaagc cgcgcaggtt attacgtact tcgtcgcctt tacgcaccag atagttggcg
    57901 atatagcaaa acagcgacag ttttgtcagc tccgcaggct ggatacgcag caaaccgaga
    57961 tcgatccaac gcgatgcccc tttaaccgag ctacccacta ccagaacgat catcagcagg
    58021 atgatagatc cgagcagcat cgtggcactg tagcgttgcc agaactccat cggcagacgc
    58081 agcgtaatga tcgccagaat aaacgccaaa atcagataga caccatcacg cttcgcgaag
    58141 aagaacggat cgttggttaa gcgttgccct atgggcattg acgccgaggt caccatgata
    58201 aagccaatcg ccgcgaggcc gaaggtcagc cacagtaagg tgcgatcgta catgatcagg
    58261 ctgtcggtat ctttttcccg cgagcccatc acccagccct ttagcgccgt ggagatccag
    58321 accaggatac tgaatcctgg caggcgcggc attttcaggc gagggagaga taaacgcatc
    58381 aacctaactc cttcgccaga cgggcaaact cattgcctcg ttgttcaaag ttcttgaact
    58441 gatcaaggct ggcacaggcc ggggagagca gaaccatatc gcccggctga acacgcggag
    58501 ccagcaagcg catcgcctgt tccatagttt cggtttgttc tgccacttcc gggcgtagcg
    58561 ccgccagctg cgcgccgtca cgaccgaaac aatacagacg tacgttatcg ccattcaggt
    58621 aacgtgccag agggctaaag tccgccgatt taccatcgcc acccagcaac aaatgcagcg
    58681 tgccgtctac gtgcaggcca ttcagcgccg cttccgtact gccgacgttg gtcgcttttg
    58741 aatcgttaat ccaacgtacg ccgttatgct ccagcacaac ttcaaagcga tgcggcagac
    58801 cagtgaatgt ggttaatgct ttcaggctgc tggcgcgcgg caaaccagct gcgtctgcca
    58861 gtgccagtgc tgcgagcgca ttggtgtagt tatgctgccc ggaaagtttc atttctttca
    58921 cgttcagcac tttctcgcct ttcacccgca gccaggtttc gccctgctga tgattcaggt
    58981 gatagtcacc catgttgacg ccaaagctga cgcagcgttc atccgcaccg cgaatcggca
    59041 ttgttaaggc atcatcagca ttaaccacgc aaactttcgc gttttcgtaa atgcgcagtt
    59101 ttgctgcacg atactgttgt aaaccaaacg gatagcgatc catatgatct tcagtcacgt
    59161 tcagaatggt cgctgctacc gcctgtaagt tggaggtggt ttccagctgg aagctcgaca
    59221 gttccagcac gtacagttca cactcatcat ccagtagcat caacgcaggc aggccaatat
    59281 tgccacccac accaacgtta acccccgccg ctttcgccat ttcacccact agcgtggtga
    59341 ccgtgctttt gccgttagaa ccggtaatcg ccacaatcgg tgcttgtgct tcgcgataga
    59401 acagctcgat atcgccaacg atttcgattc cggcatcagc ggcagcgctt agagatggat
    59461 gcgccagcgc aataccggga ctggcgacaa tcagatctgc cgccatcagc cattcatcat
    59521 tcagactgcc cgtgtggcgt tctacggctt cgggtaattt atccaggcca ggcggtgtca
    59581 tacgcgtatc cataacgcgc ggcgtcacac cgcgagcgag gaaaaagtcc acgcaggaaa
    59641 gcccggtgag gcccaggccg ataatgacga catttttacc ctgataatca gccatgatta
    59701 acgtaccttc agcgttgcca gaccaatcag aaccagcatc agcgaaataa tccagaaacg
    59761 cacaatgacg cgcggttccg gccagccttt cagttcatag tggtgatgaa tcggtgccat
    59821 gcggaaaata cgttgtccgc gcagtttaaa ggagccgacc tgcaggatga cagaaagcgt
    59881 ttctaccacg aacacgcccc ccataatcac cagcaggaat tcctgacgta gcagtacggc
    59941 gataatgcct aacgcaccac ctaacgccag cgaacctaca tcgcccataa agacctgcgc
    60001 cggataggtg ttaaaccaca ggaagcccag tcctgccccg actatcgcgg tacagacaat
    60061 aaccagttcc ccggcgtgtc gcagatacgg tatatgcaag tagctggcaa agttcatatt
    60121 gccggtcgcc cacgccacca gcgcaaaacc accggcgaca aatacggtcg gcataattgc
    60181 caggccgtcg agaccatcgg tcaggtttac cgcgttgcca gtacccacaa tgacgaagta
    60241 agccagcaga atgtagaaca gccccagctg cggcatcaca tctttaaaga atgggaccac
    60301 cagctgcgtt gcgggcgtgt ctttgccggc aaggtacagg gcgaaggcga cacccagcgc
    60361 aatgaccgac atccagaaat acttccaacg agcgatcaac cctttggtgt ctttacgcac
    60421 cactttgcga taatcatcaa caaagccaat aacaccgtaa cctaccagca ccaccaacac
    60481 gcaccagacg tacggattgg acgggtaagc ccacagcagt acggagatca caatcgccgt
    60541 caggatcata atcccgccca tggtcggcgt accgcgcttg ctgaagtgtg attcaggacc
    60601 gtcgttacgc accacctgac caaaggaaag tttttgcaaa tgagcaatca tacgcgggcc
    60661 catccacaat gagatgaaca gcgcggtcag caggctgacg atggcgcgaa acgtcagata
    60721 ggaaaagacg ttaaagccgg aataatattt gaccaaatgt tcggccagcc aaactaacat
    60781 gtcccattct cctgtaaagc gcgtactacc tcttccatgg cggcactacg tgaaccctta
    60841 actaaaatcg taattacctg ttgctcagca atcagtgatt taagacgcgt aattaacgca
    60901 gttttatcag caaaatgttc gccaacgccg ctggcggtgc tgatagcatg gctttgttta
    60961 cccacgctta acacgcggtc aataccagca gcttttgccg cctcgcccac ctgtacatgg
    61021 caggcttcgc tttcagcgcc cagttccgcc atatcgccca ccaccagcac gcggtagccc
    61081 ggcatttcag ccagtacctg gactgctgca gtcattgaac cgacattggc gttgtaggag
    61141 tcgtcgagca gcaactggtt ttctgccagt tggatgggga acagacggcc tggaacagct
    61201 ttcagatttg ccagccccgc tttgatagca tcaagcgttg cgcccacgga catggagagc
    61261 gcagcggctg ccagcgcatt cgcaatattg tgacgccccg gcaacggcag cagaacatcg
    61321 acgctaccgg ttggggtttg tagggtaaat tccgtaccgt gcgaggtcac atggatattg
    61381 gtggcggtga aatcgctgtt ggcggcattg ggtgagaaac gccacacttt gcgtgagcca
    61441 attacgctct gccagttcag ccagtcgttg ttgtcggcgt tcataatggc gataccgttt
    61501 tccggcaggc cgctaaagat ttcacctttc gctttcgcga cacccgcaag cgagccaaaa
    61561 ccttccagat gcgccgctgc caggttgttg accagcgcag cttccgggcg agtcagactc
    61621 acagtccagg ctatttcgcc ctgatggttc gcgccaagtt caataactgc gtaatcgtat
    61681 tccggcgtta agcgcaacag cgtcatcggt acaccgatgt cgttgttgag attgcctgcc
    61741 gtataaagcg tgttgccgca ctggcttaaa atcgccgccg tcatctcttt aacggaggtt
    61801 ttgccggagg accccgtcag agcaaccacg cgcgccggaa cttgctggcg aacccatgca
    61861 gccagttcac caaacgccag acgcgtatcc ttgacgatta actgcggcag gtcgatgtcc
    61921 agcggacggc taaccagtag tgcgcctgcg ccgccagctt tcgcctggtc ggcaaaatcg
    61981 tgggcatcaa aacgttcgcc tttcagggca acaaacaggc agcccggcgt cagttttcgg
    62041 gtatcagtgg ttacagcatc aagggtgata tctgcacctt gcagttcacc gttgagaatg
    62101 tcggtaagtt ggctaagggt tacgctaatc atgcaatcac ccccagcaga cgcgccaccg
    62161 tgacgcgatc ggagtagtcc agacgctgat tgccaacaat ctggtaatct tcatggcctt
    62221 tgcccgcgac cagtaccaca tcattctctt tagcctgcat aacggcgcaa gtcaccgctt
    62281 cagcacggcc ttccatcact ttggcatgtc cggcatctaa cattcccgcc agaatatcgt
    62341 tgatgatggc acgcggttct tcggtacgcg ggttatcgtc cgtcaccacc gccacgtcag
    62401 caaactcttc ggcaattgcg cccatcagtg gacgcttacc tttatcgcga tcgccaccac
    62461 agccaaagac acaccacagc ttgcccgcac agtgcagacg cgccgcctgt aaggcttttt
    62521 ccagtgcatc cggcgtatgc gcgtaatcca ccaccaccgt cggtttgcct ggcgcagtga
    62581 acacttccat acgtccgcaa accggttgca gacgcgcggc ggttttcagc agatcagcca
    62641 gtggatagcc gagtgccaac agtgtcgcca gcgcgagcag caggttgctg acgttaaaag
    62701 cgcccatcag atggctttca atttcgccat cgccccaact tgagctaaag cgaatcgtcg
    62761 caccgctgtc gtgatagttc acttcggtcg ctttcaacca gcgtccgtga cagttcggat
    62821 taatatgatc ttccattgat accgcaaccg cgtccggcag ttttgccagc cagcggcggc
    62881 ccacttcatc gtcggcgtta ataatcgcct gaccgcaatg atgctcagaa taaagcagcc
    62941 atttcgcggc ttcgtagtgt tccatatcac catgataatc aaggtgatcg cggcttaagt
    63001 tggtaaagac cgacgccgca aatttcaatg ccgccacacg gtgctgtacc agcccgtggg
    63061 aggaaacttc cattgcgcaa aacgtcgcgc cctgatccac cagccccgcc agctcatgct
    63121 gaacatcgac tgccgaaccg gttgtatttt ctgtcgggat cactttcccc agcaggccgt
    63181 taccaacggt gcccattacc gcgctggttt cgccaagcag ttggctccac tgcgccaaca
    63241 gctgggtagt cgtggttttg ccgttggtgc ccgttacgcc cacgagacgt aaattgtcag
    63301 agggttcatg gtaaaagcgg cccgccagtg cagataaacg ctcgttgagc tggctgagat
    63361 agatgaccgg tacgccgtgc atttcacgga tttcaccatc ggtcgcctca tctttcgcct
    63421 ctgcaataat ggcagccaca ccttgcgcta tcgcctgcgg gatatatcga cgcccgtccg
    63481 cctgatgacc tactacagct acaaagagat cgcccgccgc agccacacgg ctgtcgagtg
    63541 tcatctctcg cagtgctcgc gaaggtgcgt ctggcaccca cggagcaaga aggtcgcgca
    63601 aattacgatc tgccacctgt cccctcgcct tgattaatca caaattcatt tttatcgccc
    63661 gttgtcagcg catccggctc gatgttcatg gtacgcaata cgccgcccat gatggcacca
    63721 aagaccggcg cggaaacggc gccgccgtag tatttacccg cctgcggatc gttgataaca
    63781 acaaccagcg cgaagcgcgg ctgactcgca ggcgcaacgc ctgcggtata agcaatatat
    63841 ttattgatgt agcgaccgtc cggcccgacc tttttcgcgg taccggtttt aatggcgata
    63901 cgatagcctt taatcgccgc cttcacgccg ccgccgcctg gtagcgccac gctttccatc
    63961 atatgcacca cagtgcggac aatggattcc gggaagacac gttcaccggg aaccgggggg
    64021 tcaactttgg taatcgacag tgggcgataa atgccgtagc tgccgatagt tgcgtagact
    64081 cgcgctaact gtaacggtgt taccattagc ccgtagccga aagagaaggt ggccctctct
    64141 atgtcagacc accgttgttt ttgaggatat aagccactgc gttctccgac caaccccaaa
    64201 ttggtcgctt ttcccagtcc aaaacgtgag taagtatcta ctaacgctga ggacggcatc
    64261 gctaacgcca gcttggaaac accgacgtta ctcgacttct gtaatacccc ggtcagggtt
    64321 aattcgctgt agcgtgccac gtctttgatt tcgtggccgt taattcgata aggaatggta
    64381 ttgagtaccg agttttcccg caccacgcca cgttgcaacg cggtcattac caccatcggt
    64441 ttaaccgttg agcccggttc aaacacgtcg gtgatggtac ggttacgcat cgcctctttc
    64501 ggcgtgccgc tcagattgtt agggttgtat gacgggctgt tagccatcgc cagcacttca
    64561 ccggtgttga catccaccag cacggcgcta ccagattcag ccttgttaaa ggccaccgcg
    64621 ttgttcagtt cgcgataaac cagcgcctgc aggcgttcat caatactcag cgccaggttg
    64681 tgcgctgcct ggctgtcagt agaagaaata tcttcaatta cgcgaccata gcggtcttta
    64741 cgcacaatgc gctcacccgg ctgcccggta agccatttat cgaaactctt ctcaacgccc
    64801 tcaatccctt gactatcgac gttagtaaag ccgatgaggt gagcagtcac ttcgccggac
    64861 ggatagtaac ggcgagactc ttcacgcaga tgaatccccg gcagtttcag ttttttgatg
    64921 tagtccgcca tgtcagggtt cacctgacgc gccagataaa taaagcgccc tttcgggttg
    64981 gcgttaatgc gggctgaaag ctgatccagc ggaatattga gcgcgttagc cagcgccttc
    65041 cagcggtcac cgacgctgat accgccagcg tcatgcactt ctttcgggtc agcccaaatc
    65101 gcttttaccg gcacgctcac cgctaacggg cgaccagaac ggtcagtaat catgccgcgg
    65161 gaggtggaaa cttgctgaac gcgaagagaa cgcatgtcgc cctctttcac cagcatatcc
    65221 ggggagataa cttgtaacca cgctacgcgt ccgagcagaa aagccagcgc caggagaata
    65281 cagccgcata acaacgcaaa acgccaactg ataaagttgg catgttcttc ctgacgtttt
    65341 ggtttctgcg ttttcgccgc tgctttcatg cgtcgcgttt atccttattt ttgcactacg
    65401 atattttctt gtgacggatc aacatgctgc atttgcagct tttccgtggc gatcctttcc
    65461 acccggctat ggtcgccgag cgcattctct tcaaggatca ggttgcgcca ttcaatgtct
    65521 aaagcatctc gctccagcac cagttgttcg cgctgagcgg tcagtaaacg ggtatggtgc
    65581 gccgtggtta ccacagtcac cgccgtcaaa ataatgcaaa tgaacaggca gagtggcagc
    65641 ttcccaaatc gcaaaagatc gtcaccgata acaccaggca atgcatggcg ctcgtggctt
    65701 cccatcgatc ctttaacttt gcttagagct tctgtcactc tgctgatcat gcattcgtcc
    65761 tctctgcaat acgcagaact gaactacggg cacgagggtt ctcagccacc tcttcttcgc
    65821 ccggcattaa cttgcctagt gctcgcagct gacggccacc cagttttttg agctgctctt
    65881 cagtcatcgg taaccctgcc ggaacttgcg gaccgcggct gttttcacgc ataaaacgtt
    65941 tcacaatacg gtcttccagc gagtggaagc tgatgatcga aagccgccca cccggggcca
    66001 gcacgttgag cgagcttttt agcgcctgct ctatctcctc cagttcactg tttacccaaa
    66061 tgcgcaccgc ctggaaggta cgggtcgcgg gatgtttaaa cttatctttc accggcgttg
    66121 cagcagccac gacttccgcc agttctttgg tgcgggtcat cggctgttcg cggttacgct
    66181 cgacaatggc gcgggcaatg cgtttggcaa aacgctcttc accataggtt ttcaataccc
    66241 aggcgatatc ggcttcttct gcggtttgta gccattcagc ggctgactgc ccacgggttg
    66301 ggtccatacg catgtccagc ggaccatcgc gcataaagga aaagccgcgc tcagcatcat
    66361 caagttgcgg tgaagagacg ccaagatcga ggagaatgcc gtcgatcttg ccgataagat
    66421 cgcgctcggc aacgtattcg cccagcgcgg aaaaaggtcc gtggatgatg gagaagcgcg
    66481 gatcatcaat agtcttcgca acggcgatag cctgcgggtc gcgatcgatc gccagcaaac
    66541 gcccctcttc gccaagctgc gagaggatca gacgtgagtg accaccgcga ccaaaagtcc
    66601 catcaatgta gatgccatca ggacggatat tgaggccatt aacggcttca tccaacagca
    66661 ccgtagtatg tttatagttt tccatcattt tatagagaca agtcctgcag tcgctccgat
    66721 aagtctccgg tagccaactg ctctgcgtcg atatcttcct tgacctgttg atgccaggtt
    66781 gtttcatccc acagctcaaa cttgttgaac tgtccaacca gcatcacttc ttttgtcagc
    66841 ccggcatgtt gccgcagtac tggcgcgatt aacaatcgac ctgcgccatc catctgacat
    66901 tcgctggcat gacctaacag taggcgctgc acacggcgct caaccgggtt catgctcgac
    66961 agacgcgata atttttgctc gataatttcc cattcaggca gggggtaaag cagcaggcac
    67021 gggtgatgaa tgtcaatggt gcaaaccatt tgaccggcag cgttctcaag cagctgttcc
    67081 cggtaacggg taggcactga taagcgccct ttgctgtcga gattgactaa cgttgctccc
    67141 cggaacatgc cagcctcacc ccttattccc actttgcccc acaatttccc actgaaagga
    67201 gtttacggag cggaggaaaa gcttgtcaag ccagtcacaa ggcataccga ggtaaaaaac
    67261 ccgatgttta cggctaatat cagcgttgtt tattttacgt ccaacgtgcg cagcaaatta
    67321 acgttatgaa tagctgtaag aaaaaatccc tcatcgtcat caccgtttta aaatgactca
    67381 ctattttcca cagcaaatat aaagtgtcag tttgcgacgc gagcggcatt ttaggacata
    67441 tcttccccgg ttaacagtcc ctgttgcgtc tgtgtggcgc ttgatgccag cgggtttgcg
    67501 caaaagcaaa gaaaagtgct tgttaaatcg gcaattcatc tctgcatgta acaaaataat
    67561 gcaaaaacgc gctgtcgata tcacttaatt attgtgaatt tcagctggtg agttaattaa
    67621 cgggcgggtt taaaagaact tatcaataat agtcaggaat agtcttattt actttaagga
    67681 tattgatgtc cattcccgta ctctacgcgc cagagggaaa ttcacctggc gcgtattttt
    67741 gttcgcggct tagctacggc tgagcacgcc gcggcgatag agattacgtt taatgcgcgt
    67801 taaaccaggt tttggcttac gcggttcgtc caggcttgcc aggacaatct ccagcacacg
    67861 ctctgcgaca tcgcggtgac gttgagccac tgccagcacc ggacactgta agaagtcgag
    67921 cagttcgtta tcgccaaagg tggcaattgc caggtcagaa ggcagtttgc cgtcgcgacg
    67981 cagcgtgaca tccatcactc cttgcaacaa cgcaaacgac gttgtgaaca gcgcctgcgg
    68041 catcggatgc gtttccagcc atttttcgaa taactgggca gccgcctccc gctcatagct
    68101 gttggcatac aggaaatgca cttcgcgcgg atcatctttc caggcagtac ggaaaccttg
    68161 ttcacgcagg aagctgacag aaagctccgg tagcgcacca agataaagca ccgtctcggc
    68221 gggaaactta cgtaactctt ccgccagcat ttcggcatca tcctgatcgg caccaaccac
    68281 gctggtgaag tgttcacgat cgagggcgcg gtccagcgcg acaatcggga acgggtcgtt
    68341 agcccagcgt tgataaaaag gatgctcagg aggcaacgac gtcgaaacaa taatggcatc
    68401 aacctgacgc tgtaaaaggt gctcaatgca ccgcatttcg ttgtctggct gatcttctga
    68461 gcaggcaatc agcagttgat aaccccgttg ccgcgcctgg cgttcaagat agttagcgat
    68521 gcgggtatag ctggtgttct ccagatcggg gatcacaaga ccaatagaac gtgtgcgtcc
    68581 agcacgaagc ccagctgcca cggcgttcgg gtggtaattg tgctcacgca ccacagccat
    68641 gactttttca acggttttgt cgctcacacg gtattgcttc gctttgccgt taataacata
    68701 gcttgcagtg gtccgcgaca ctcccgccag ccgagcgatt tcatccagtt tcacaattgc
    68761 cccttgcgta aaatgtaaaa accatagcca ttgtactggt atgggttaaa ttcattaaca
    68821 tctaagcgca gaaaattaac tgcggcaact gcttttattc caggttccca ctgatttcgc
    68881 aaaaaaagcc caacgtgaca tgttgggctg taaattgcgc attgagatca ttctcaacgc
    68941 attattttat cgccgcgcga aagtccgacc acaccagagc gagcaacctc cacaattttc
    69001 gccacatcgc gaatcgatgc taaaaatgca tcaagcttac cgctggtgcc tgctaattga
    69061 acggtataaa gcgagggtgt gacatcgata atttgcccac ggaatatttc cgtattacgt
    69121 ttcacttcgt cacgcccgta accgctggcc tgaattttca ccagcatgat ttcccgctca
    69181 acatgcgcgc cctgccccaa ctcactcacg cgcaagacat cgaccagttt gtgtaattgc
    69241 ttttcgatct gctcaagtac tttttcatcg cccacggtct ggatggtcat acgcgataat
    69301 gtcggatcgt cggttggcgc aacggtcagg ctttcaatgt tgtagccacg ctgggaaaaa
    69361 aggccaatca cgcgggataa cgcgcctgat tcattttcga gtaagactga taatatccgg
    69421 cgcataatca ggttctctcc gttttgctta accacatttc atccattccg cccccgcgaa
    69481 tctgcatcgg gtagacgtgc tcgctgccat cgacggtaac atcaacaaac accaggcgat
    69541 tattgcgcac ctgttccagc gcctcgctaa gtttgctttc cagctcatgc ggatgagaaa
    69601 tctggatccc gacatgccca taggcttccg ccagacggac gaaatcgggt agcgattgca
    69661 tataagattg tgaatgacgg ccggaataga tcatgtcctg ccactgcttc accatcccca
    69721 gatagcggtt attgagattc accaccagta cgggcaactc gtattgcaac gcggtagaca
    69781 gttcctggat gttcatctga atactgccgt cgccagtgac gcaaaccacg gtttcttctg
    69841 gcaacgccat tttgacgccc agtgccgcag gtaaaccaaa acccatcgtg ccgaggccac
    69901 cggaattgat ccagcgacgc ggtttgtcga atggataata aagtgcagca aacatctggt
    69961 gctgcccgac atcggacgtc acgtaagcgt ctcccttcgt caaccgccaa agagtctcga
    70021 tcaccgcctg cggtttaatc ttttcactgt gagtgtcata tttcaggcac tgacgagcgc
    70081 gccactgttc aatttgctgc caccagtcgc ggatctcatc cagtggttga tgggcggatt
    70141 cttgcgacaa gagttcaagc atttgttcga ggacctggcg agcatccccc acaatcggga
    70201 tatccgcagt cacggtttta gaaatggaag taggatcaat atcgatatgc agaacagtgg
    70261 catttgggca gtactttgcc agattgttcg tcgttcggtc atcaaatcgt accccgacgg
    70321 cgaaaatcac atccgcgtta tgcatcgtca tattggcttc gtaggtaccg tgcattccca
    70381 gcatgcccag tgcctgacga tgcgttgccg gaaacgcccc cagccccatc aatgagcaaa
    70441 caacgggcag attcaacgcc tccaccgttt ctttcaactg ctgatggcag cccgccgtga
    70501 ttgccccacc gcctacgtag acaaccggtt tttttgccgc taccagcgtt tgcagagcac
    70561 gcttaatttg ccctttatgt ccggtagtag tgggattgta agaacgcata ctgaccgact
    70621 ccggccagac atagggtaat ttgttcgccg gattaagaat atctttcggt aaatcaacga
    70681 ctactggtcc tgggcgacca cttgccgcca gccagaaagc ctttttcagc acctgcggaa
    70741 tgtcttccgt ttgcttaacc agaaaactgt gtttaaccac cggtcgcgaa atccccacca
    70801 tgtcgcactc ctgaaaggca tcgtaaccta tcaacgaggt cgctacctgc ccggaaagga
    70861 caactaatgg aatggaatcc atataagcgg tggcgatgcc agtaatcgca ttggtcgccc
    70921 ctggacccga cgttaccagc acgacgccga cttccccggt cgcgcgcgcc aggccatcgg
    70981 ccatatgcac cgccgcctgc tcatgacgaa ctaatacatg atcaatacca cccacggtat
    71041 gcaatgcatc ataaatatca aggactgcgc ctccgggata accgaatact tgtttaacgc
    71101 cctgatcgat aagcgatcgg acgaccatct cggctccaga caacatctcc atggcctgcc
    71161 tcactgtttg acggaaaaaa tgtgtaaaac acattaaccg tccgatcttg cttaagcaat
    71221 tggcagccag agacataaac acggggtaat aagaataaac aggaggaaag gtgaaaagaa
    71281 acgataaaat cctccattac agaggattaa attgataata aatcggctga atcccacaac
    71341 ttacagcaat ttctctattt ttttaattaa tcctaaaata gagagcccac acattcagaa
    71401 taaaattcta cattcaataa aaaatgcaga ataaaccaga cattcatgtc tgacctattc
    71461 tgcaatcagt tagcgtttgc aaattgagac taattgctct tccatccact gatggccttt
    71521 atcgcgcccg gcagcttcat gccaggagag ataacaggtt ctgctgtttt gttttaacgg
    71581 cagcggtaat acctgtaatt ctaaggattc agcgaactct tcagccagcc aacgcggcgc
    71641 aatagcgacc aaatgcgttt gcgacaccac gctaagtacg ctcatcattg ccatgccctg
    71701 atacgcgata ctggcttgct tatctaccgt gtcataccaa ggttgactaa atgacgcgaa
    71761 acgatcgagc gaaaccgccg catgttgttc gttataaaca tcatgtttca gtaacgggcc
    71821 cttaattgtt ggatgatttt tgctggctac cagcaccatt tcatctttaa ataatggtac
    71881 gctggtaaat tcaggacgat ggaagtcttc ataactaatc acaaactccg tttcctgata
    71941 acgcagctga tgttcagtgt tctgatttaa tgaagacttg aacataacat gtatatttgg
    72001 cgcaatctgc tcaatgtgat tataaatctg cgaggtcaga atgctgtcta acgggctgca
    72061 aacacaaaga tgaaatacac gttcactgct cgcgggttca aaacctgaac caggcaattc
    72121 attttgtact agttgcaatg cctgacgaac tgaaccaaaa agttgaaatg cgcgagcagt
    72181 cggttgaata ccacggccat aacgaacaaa aagctcgtca ttaaacatca ccttcaggcg
    72241 tgcaacagcg ttactgaccg caggttgcga cattcccaga acatgagcgg cacgagtaat
    72301 gttttgctcc tgcatcacgg catcgaaaac ggttaataag ttgagatcga ccatgcgtag
    72361 ctgtggcttg cttaactccg ccgtctctgg atgatctgtt tgtacctctg gcatacttaa
    72421 ctccactgtc acacttaact ccctttccct tattggaatg cagagaaata atcctcgaga
    72481 atatgattta tcatgcatat aaaataagaa aaaggaaaat gagtaaaatt aggaaaacat
    72541 aaagatacgg gttctgataa aaacagaatc cattgccgtg gaatgagtca tttaccacag
    72601 cataataatc cataatgttt ttgttgcgaa aacaatctaa tcataactac cgcgaatact
    72661 caatcatcta caaaatggat taaatgtgct tatttaataa ttaatttata tatttaatgc
    72721 attaattctt aacattaatt gatcaataat attcaccaaa tcaatatcaa aaaaaaatcg
    72781 caaaacatat aattcaatac aaatcatcag gataggtttt gcaacgcgtg cattttgtcc
    72841 cctttttcct cgttgattag atgcaaaaat ttatgctgaa atatgtcaac cgatgaaaag
    72901 cgtcggtagt taagcagaaa ttaatatcgc ttactttaac caccgcagca caattagcta
    72961 attttacgga tgcagaactc acgctggcgg gacgttttta ttgcgtcagg gttgacatcc
    73021 gtttttgtat ccagtaactc taaaagcata tcgcattcat ctggagctga tttaatgact
    73081 cacatcgttc gctttatcgg tctactacta ctaaacgcat cttctttgcg cggtagacga
    73141 gtgagcggca tccagcatta agccagcacg cagtcaaaca aaaaacccgc gccattgcgc
    73201 gggttttttt atgcccgaag cgaggcgctc taaaagagac aaggacccaa accatgagcc
    73261 agcaagtcat tattttcgat accacattgc gcgacggtga acaggcgtta caggcaagct
    73321 tgagtgtgaa agaaaaactg caaattgcgc tggcccttga gcgtatgggt gttgacgtga
    73381 tggaagtcgg gttccccgtt tcttcgccgg gtgattttga atcagtgcaa accatcgctc
    73441 gccaggttaa aaacagccgc gtatgcgcgt tagctcgctg cgtggaaaaa gatatcgacg
    73501 tggcggccga atccctgaaa gtcgccgaag ccttccgtat tcataccttt attgccactt
    73561 cgccaatgca catcgccacc aagctgcgca gcacgctgga cgaggtgatc gaacgcgcta
    73621 tctatatggt gaaacgcgcc cgtaattaca ccgatgatgt tgaattttct tgcgaagatg
    73681 ccgggcgtac acccattgcc gatctggcgc gagtggtcga agcggcgatt aatgccggtg
    73741 ccaccaccat caacattccg gacaccgtgg gctacaccat gccgtttgag ttcgccggaa
    73801 tcatcagcgg cctgtatgaa cgcgtgccta acatcgacaa agccattatc tccgtacata
    73861 cccacgacga tttgggcctg gcggtcggaa actcactggc ggcggtacat gccggtgcac
    73921 gccaggtgga aggcgcaatg aacgggatcg gcgagcgtgc cggaaactgt tccctggaag
    73981 aagtcatcat ggcgatcaaa gttcgtaagg atattctcaa cgtccacacc gccattaatc
    74041 accaggagat atggcgcacc agccagttag ttagccagat ttgtaatatg ccgatcccgg
    74101 caaacaaagc cattgttggc agcggcgcat tcgcacactc ctccggtatc caccaggatg
    74161 gcgtgctgaa aaaccgcgaa aactacgaaa tcatgacacc agaatctatt ggtctgaacc
    74221 aaatccagct gaatctgacc tctcgttcgg ggcgtgcggc ggtgaaacat cgcatggatg
    74281 agatggggta taaagaaagt gaatataatt tagacaattt gtacgacgct ttcctgaagc
    74341 tggcggacaa aaaaggtcag gtgtttgatt acgatctgga ggcgctggcc ttcatcggta
    74401 agcagcaaga agagccggag catttccgtc tggattactt cagcgtgcag tctggctcta
    74461 acgatatcgc caccgccgcc gtcaaactgg cctgcggcga agaagtcaaa gcagaagccg
    74521 ccaacggtaa cggtccggtc gatgccgtct atcaggcgat aaaccgcatc actgactata
    74581 acgtcgaact ggtgaaatac agcctgactg ctaaaggtca cggtaaagat gctctgggtc
    74641 aggtggatat tgtcgctaac tacaacggtc gccgcttcca cggcgtcggc ctggccaccg
    74701 atattgtcga gtcctccgcc aaagccatgg tgcacgtact gaacaatatc tggcgcgccg
    74761 cagaagtcga aaaagagttg caacgcaaag ctcaacacaa cgaaaacaac aaggaaaccg
    74821 tgtgatgtcg aagaattacc atattgccgt attgccgggg gacggtattg gtccggaagt
    74881 gatgacccag gcgctgaaag tgctggatgc cgtgcgcaac cgctttgcga tgcgcatcac
    74941 caccagccat tacgatgtag gcggcgcagc cattgataac cacgggcagc cgctgccgcc
    75001 tgcgacggtt gaaggttgtg agcaagccga tgccgtgctg tttggctcgg taggcggtcc
    75061 gaaatgggaa catttaccac cagaccagca accagaacgc ggcgcgctgc tgcctctgcg
    75121 taagcacttc aaattattca gcaacctgcg cccggcaaaa ctgtatcagg ggctggaagc
    75181 attctgtccg ctgcgtgcag acattgccgc aaacggcttc gacatcctgt gtgtgcgcga
    75241 actgaccggc ggcatctatt tcggtcagcc aaaaggccgc gaaggtagcg gacaatatga
    75301 aaaagccttt gataccgagg tgtatcaccg ttttgagatc gaacgtatcg cccgcatcgc
    75361 gtttgaatct gctcgcaagc gtcgccacaa agtgacgtcg atcgataaag ccaacgtgct
    75421 gcaatcctct attttatggc gggagatcgt taacgagatc gccacggaat acccggatgt
    75481 cgaactggcg catatgtaca tcgacaacgc caccatgcag ctgattaaag atccatcaca
    75541 gtttgacgtt ctgctgtgct ccaacctgtt tggcgacatt ctgtctgacg agtgcgcaat
    75601 gatcactggc tcgatgggga tgttgccttc cgccagcctg aacgagcaag gttttggtct
    75661 gtatgaaccg gcaggcggct cagcaccaga tatcgcaggc aaaaacatcg ccaacccgat
    75721 tgcgcaaatt ctgtcgctgg cactgctgct gcgctacagc ctggatgccg atgatgcggc
    75781 ttgcgccatt gaacgcgcca ttaaccgcgc attagaagaa ggcattcgca ccggggattt
    75841 agcccgtggc gctgccgccg ttagtaccga tgaaatgggc gatatcattg cccgctatgt
    75901 ggcagaaggg gtgtaatcat ggctaaaacg ttatacgaaa aattgttcga cgctcacgtt
    75961 gtgtacgaag ccgaaaacga aaccccactg ttatatatcg accgccacct ggtgcatgaa
    76021 gtgacctcac cgcaggcgtt cgatggtctg cgcgcccacg gtcgcccggt acgtcagccg
    76081 ggcaaaacct tcgctaccat ggatcacaac gtctctaccc agaccaaaga cattaatgcc
    76141 tgcggtgaaa tggcgcgtat ccagatgcag gaactgatca aaaactgcaa agaatttggc
    76201 gtcgaactgt atgacctgaa tcacccgtat caggggatcg tccacgtaat ggggccggaa
    76261 cagggcgtca ccttgccggg gatgaccatt gtctgcggcg actcgcatac cgccacccac
    76321 ggcgcgtttg gcgcactggc ctttggtatc ggcacttccg aagttgaaca cgtactggca
    76381 acgcaaaccc tgaaacaggg ccgcgcaaaa accatgaaaa ttgaagtcca gggcaaagcc
    76441 gcgccgggca ttaccgcaaa agatatcgtg ctggcaatta tcggtaaaac cggtagcgca
    76501 ggcggcaccg ggcatgtggt ggagttttgc ggcgaagcaa tccgtgattt aagcatggaa
    76561 ggtcgtatga ccctgtgcaa tatggcaatc gaaatgggcg caaaagccgg tctggttgca
    76621 ccggacgaaa ccacctttaa ctatgtcaaa ggccgtctgc atgcgccgaa aggcaaagat
    76681 ttcgacgacg ccattgccta ctggaaaacc ctgcaaaccg acgaaggcgc aactttcgat
    76741 accgttgtca ctctgcaagc agaagaaatt tcaccgcagg tcacctgggg caccaatccc
    76801 ggccaggtga tttccgtgaa cgacaatatt cccgatccgg cttcgtttgc cgatccggtt
    76861 gaacgtgcgt cggcagaaaa agcgctggcc tatatggggc tgaaaccggg tattccgctg
    76921 accgaagtgg ctatcgacaa agtgtttatc ggttcctgta ccaactcgcg cattgaagat
    76981 ttacgcgcgg cagcggagat cgccaaaggg cgaaaagtcg cgccaggcgt gcaggcactg
    77041 gtggttcccg gctctggccc ggtaaaagcc caggcggaag cggaaggtct ggataaaatc
    77101 tttattgaag ccggttttga atggcgctta cctggctgct caatgtgtct ggcgatgaac
    77161 aacgaccggc tgaatccggg cgaacgttgt gcctccacca gcaaccgtaa ctttgaaggc
    77221 cgccaggggc gcggcgggcg cacgcatctg gtcagcccgg caatggctgc cgccgcggct
    77281 gtgaccggcc atttcgccga cattcgcaac attaaataag gagcacacca tggcagagaa
    77341 atttatcaaa cacacaggcc tggtggttcc gctggatgcc gccaatgtcg ataccgatgc
    77401 aatcatcccg aaacagtttt tgcagaaagt gacccgtact ggttttggcg cgcatctgtt
    77461 taacgactgg cgatttctgg atgaaaaagg ccaacagcca aacccggact tcgtgctgaa
    77521 cttcccgcag tatcagggcg cttccatttt gctggcacga gaaaacttcg gctgtggctc
    77581 ctcgcgtgag cacgcgccct gggcattgac cgactacggt tttaaagtgg tgattgcgcc
    77641 gagttttgct gacatcttct acggcaatag ctttaacaac cagctgctgc cggtgaaatt
    77701 aagcgatgca gaagtggacg aactgtttgc gctggtgaaa gccaatccgg ggatccattt
    77761 cgacgtggat ctggaagcgc aagaggtgaa agcgggagag aaaacctatc gctttaccat
    77821 agatgccttc cgccgccact gcatgatgaa cggtctggac agtattgggc ttaccttgca
    77881 gcacgacgac gcaattgccg cttatgaagc aaaacaacct gcgtttatga attaatcccc
    77941 ttgcccggtc aaatgaccgg gctttccgct atcgtccacg tcatcaaatg tctttaacct
    78001 ttgcggttaa aaataatgcg acaacagaaa taaccgcaat tatccagtag acagcaaagt
    78061 gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc ccggtagaaa
    78121 tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa tcctgaaacc
    78181 atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt tgcagcgtca
    78241 tcaacgccag acggctatga aataaaatca atccggtgta aaacagtact cctgccgcca
    78301 ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca gccagaatca
    78361 ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt ttgtctggca
    78421 atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg ttgcaggtcc
    78481 acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag ccaccttgca
    78541 ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc ataaatgcaa
    78601 tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc gtaaagccgt
    78661 aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga gaaagctgcg
    78721 cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca tattcccgcg
    78781 ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc acaccacagg
    78841 tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg atagccatca
    78901 aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt tttgccaacc
    78961 agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg atccagaaag
    79021 gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt agcgccccgg
    79081 ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc atacggcgag
    79141 ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata aaaaaaacca
    79201 gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca tgatgcaggc
    79261 aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca tggttaatcg
    79321 ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa aatagtgctg
    79381 ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg acttaatata
    79441 gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat ctgctcgtct
    79501 gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg acacaacgct
    79561 caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc tgctcaacac
    79621 catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta aacgctcgcg
    79681 tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag acctgctgga
    79741 gcaggatcgt atcgaccaac tagtacagtt ggttggcgac aaagcgactg tgcggcaaat
    79801 gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc gcgtgctcta
    79861 ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg aaacccatat
    79921 tgcccggcaa atcttcagtt cgctaacgcg cataaatgag gaaaatgggg aactggaagc
    79981 agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct ttttgcgtcc
    80041 aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg cctctttaaa
    80101 acgaatcaat acgctgccgc tctattcgca tattgctgac attgtgtcgc cgacgccctg
    80161 gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttattgc tggggcaagt
    80221 tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca gccatcccat
    80281 cggcaccggt ccgtatgcgg tgatccgcaa cagcaccaat caactgaaaa ttcaggcatt
    80341 cgatgacttc ttcggttacc gggcattaat cgacgaagtt aacgtctggg ttctgccgga
    80401 aattgccgac gagccagccg gaggtctgat gctaaaaggt ccacagggcg aggaaaaaga
    80461 gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc gcacccatcg
    80521 cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa ctaatctggt
    80581 ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac tgctcccccg
    80641 ttggcaccat gcccgcacca taaagagcga aaaaccggct ggtctggaaa gcctcaccct
    80701 aaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc agcagattct
    80761 ggcaagtcac caggtcacgc tggaaatcaa agagatcgac tacgatcagt ggcatacagg
    80821 agagatcgaa agtgatatct ggctaaacag cgccaacttt accctgccgc tggacttctc
    80881 tgttttcgca catttatgcg aagtgccact gctacaacat tgcattccca ttgactggca
    80941 agccgacgct gctcgctggc gcaatggcga gatgaatctg gcgaactggt gccagcaact
    81001 ggtcgccagc aaagcgatgg tgccattatt gcaccactgg ctgatcattc aggggcaacg
    81061 cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat cagcgtggtt
    81121 tgcgccaccg gatccatgat tgctggttga taacaaaatc actacactaa cgccgttctc
    81181 aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg atccggataa
    81241 cgccggcgaa gggatttgag gctccttctc aagtcctttg ccactctttt ttgaggtgca
    81301 aagtgttaaa aaaatgtctg cccctgctgt tgctgtgcac agcgcccgtt ttcgctaaac
    81361 ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct ggtccggtgg
    81421 ttaaaaaagc ctttgaagcc gactgtaatt gcgaactgaa actggtggcg ctggaagatg
    81481 gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa cagcaaagcc gatgtagttc
    81541 tggggctgga taacaacctg ttagacgccg ccagtaaaac cggactgttt gccaaaagcg
    81601 gcgtggcagc ggatgccatt aacgttcccg gcggctggaa taatgacact ttcgtaccgt
    81661 ttgattacgg ctacttcgcc ttcgtctacg acaagaacaa actgaaaaac ccgccccaaa
    81721 gcctgaaaga gctggttgag agcgatcaaa actggcgagt gatttatcag gatccgcgca
    81781 ccagtacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc gatgacaccc
    81841 cgcaggcctg gcagaaactg gcgaagaaaa ctgtcaccgt caccaaaggc tggagcgaag
    81901 cctacggcct gtttttaaaa ggtgaaagcg atctggtact gagttacacc acctctccgg
    81961 cttatcacat tctcgaagag aaaaaagata actacgccgc cgcgaacttt agcgaaggtc
    82021 actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg gagctggcgc
    82081 aaaaatttct ccagtttatg gtttctccgg ctttccagaa tgcgatccca accggcaact
    82141 ggatgtatcc ggtggcaaat gtcacgctgc ctgccgggtt tgaacaattg accaaaccag
    82201 caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca tggattagcg
    82261 aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg taagcgccgc
    82321 cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga acgcgccgca
    82381 gggtaactgg gtggcaatct ggcaggacag ctacctgtgg catgtggtgc gcttctcctt
    82441 ctggcaggcg tttctctcgg cgctgctctc tgtcgtaccc gcgatattcc tcgcccgcgc
    82501 actctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg caatgacctt
    82561 gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc gtcagggctg
    82621 gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct acggcctgca
    82681 aggtattttg ctggcgcacg tattttttaa tctaccgatg gcgagccgct tattactcca
    82741 ggcactggaa aacattcccg gcgagcaacg tcagcttgcc gcccagcttg ggatgcgcgg
    82801 ctggcatttt ttccgcttca tcgaatggcc gtggctacgg cgacaaatcc cgccggttgc
    82861 cgcgcttatc tttatgctct gtttcgccag cttcgccacc gtgctgtcgc tgggcggcgg
    82921 tccgcaggca accactatcg agctggcaat ctatcaggcg ctgagttacg actacgatcc
    82981 tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc tggtgctgtt
    83041 gagtcagcga ctaagtaagg ccattgcgcc cggcaccacg ctgctgcaag gctggcgcga
    83101 cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaattgtgc tggcgctgct
    83161 gctgttgcta ccaccgttac tggcggtgat cgtcgatggg gtaaatcgcc agttgccgga
    83221 agtgctggca caaccggtgc tgtggcaggc gctgtggacc tcgttgcgta ttgcgctggc
    83281 ggcaggcgta ttgtgcgtgg tgctgaccat gatgctgcta tggagcagtc gcgaactgcg
    83341 ggcgcggcaa aaaatgctgg ccggtcaggc gctggagatg agcggcatgt tgattctcgc
    83401 catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca ccattggcct
    83461 gccgcaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga tcccttatgc
    83521 gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagta tgttgtgtca
    83581 gtcgctgggc attgaaggct ggtcgcgctt aaaagtggtc gagctgcgcg ccctgaaacg
    83641 tccactggcg caggcgctgg cttttgcctg cgtgctgtcg attggtgatt ttggcgtagt
    83701 ggcgttgttc ggtaacgatg atttccgtac cctgccgttt tatctctacc agcaaattgg
    83761 ctcctatcgc agccaggacg gtgcggtcac cgcgttaatt ctgctactac tctgttttct
    83821 actgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact gatatcacct
    83881 ggctttatca ccatttgccg atgcgtttta gcttaacggt agaacgcggc gagcaggtgg
    83941 caatcctcgg accaagcggc gcgggtaaaa gtaccctgct gaatttgatc gccggttttc
    84001 tgacgccagc cagcggttcg ctgactatcg acggcgttga tcacaccacg acgccgccgt
    84061 cacgccgtcc ggtgtcgatg ctgtttcagg aaaacaacct gttcagtcac ctgacggtcg
    84121 cgcagaacat cgggctgggg ctaaatccgg gattgaaact gaacgcggca cagcaggaga
    84181 aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcacgg ttacctggcg
    84241 agctttccgg cggtcagcga cagcgagtgg cgttagcacg ttgtctggta cgcgaacagc
    84301 cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcactacgc caggagatgt
    84361 tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg gtgtcacaca
    84421 gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac gggcgcatcg
    84481 cctggcaggg taagaccaat gagttgttga gcggtaaggc gagtgcttca gcactattgg
    84541 ggattacggg ttagtatgca ctgtcagatg cggcgtgaac gccttatccg acctacggac
    84601 agtgcgtttt gtaggcctga taagacgcgg tcagcgtcgc atcaggcaac ccgcacaaga
    84661 caatccccta aaccccaacc actttacgca aaatatcgat atacaccggc atcaacgggt
    84721 ggcgaattaa caccaccagc gccaccacgc cgatggcaga aatcaacggc gtcagccaca
    84781 acaaacggcc gcgcgacaaa taatgactta aacgatcggt tgctttaccg ctgcgccata
    84841 accgccagca cagccagcca ccaacccaca aaaataccgc cgtcgccagc agcaaccatt
    84901 taaactcgcc gctctgcatt ccggctggaa tatcgatcgc cgcgcccgcc agaatcccag
    84961 gcaggaagta aaacggtggc cacagcaggc agccgataat attcggcgta atgaattttg
    85021 ccaccggcag atccagcatt cccgccacca ttggcaccag cggacgcgtc gggccaacaa
    85081 agcgaccgac cagaatggtg aacatgctgt gttgatgcaa cgcatgttca gttttatcga
    85141 gtaatgcttt gtttttcttc agaaatgacc agcgatgcaa cggcttttta aaacgccagc
    85201 ccagccagaa ggagatccag tcgcccagca gacaaccgac aatccctgcc agccaggcgt
    85261 gccagaaact taactcgccg ctgccaatca gcgctcccag ccccgccatc agcaccgtac
    85321 cgggtagaat caaaccgacc agcgccagcg actccagaaa ggccaccaac accaccgcca
    85381 tcaatgaata cacggtagat tgggtaataa agtgttccag caatgcttgc ataatatgcc
    85441 tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt
    85501 gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa
    85561 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag
    85621 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc
    85681 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct
    85741 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg
    85801 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt
    85861 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac
    85921 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg
    85981 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag
    86041 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga
    86101 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga
    86161 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga
    86221 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc
    86281 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc
    86341 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt
    86401 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt
    86461 cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc
    86521 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat
    86581 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca
    86641 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
    86701 tctccatacc cgtttttttg gatggagtga aacgatggcg attgcaattg gcctcgattt
    86761 tggcagtgat tctgtgcgag ctttggcggt ggactgcgcc agcggtgaag agatcgccac
    86821 cagcgtagag tggtatcccc gttggcaaaa agggcaattt tgtgatgccc cgaataacca
    86881 gttccgtcat catccgcgtg actacattga gtcaatggaa gcggcactga aaaccgtgct
    86941 tgcagagctt agcgtcgaac agcgcgcagc tgtggtcggg attggcgttg acagtaccgg
    87001 ctcgacgccc gcaccgattg atgccgacgg taacgtgctg gcgctgcgcc cggagtttgc
    87061 cgaaaacccg aacgcgatgt tcgtattgtg gaaagaccac actgcggttg aagaagcgga
    87121 agagattacc cgtttgtgcc acgcgccggg caatgttgac tactcccgct atattggcgg
    87181 tatttattcc agcgaatggt tctgggcaaa aatcctgcat gtgactcgcc aggacagcgc
    87241 cgtggcgcaa tctgccgcat cgtggattga gctgtgcgac tgggtgccag ctctgctttc
    87301 cggtaccacc cgcccgcagg atattcgtcg cggacgttgc agcgccgggc ataaatctct
    87361 gtggcacgaa agctggggcg gcttgccgcc agccagtttc tttgatgagc tggacccgat
    87421 cctcaatcgc catttgcctt ccccgctgtt cactgacacc tggactgccg atattccggt
    87481 gggcacctta tgcccggaat gggcgcagcg tctcggcctg cctgaaagcg tggtgatttc
    87541 cggcggcgcg tttgactgcc atatgggcgc agttggcgca ggcgcacagc ctaacgcact
    87601 ggtaaaagtt atcggtactt ccacttgcga cattctgatt gccgacaaac agagcgttgg
    87661 cgagcgggca gttaaaggta tttgcggtca ggttgatggc agcgtggtgc ctggatttat
    87721 cggtctggaa gcaggccaat cggcgtttgg tgatatctac gcctggttcg gtcgcgtact
    87781 cagctggccg ctggaacagc ttgccgccca gcatccggaa ctgaaagcgc aaatcaacgc
    87841 cagccagaaa caactgcttc cggcgctgac cgaagcatgg gccaaaaatc cgtctctgga
    87901 tcacctgccg gtggtgctcg actggtttaa cggtcgtcgc tcgccaaacg ctaaccaacg
    87961 cctgaaaggg gtgattaccg atcttaacct cgctaccgac gctccgctgc tgttcggcgg
    88021 tttgattgct gccaccgcct ttggcgcacg cgcaatcatg gagtgcttta ccgatcaggg
    88081 gatcgccgtc aataacgtga tggcgctggg cggcatcgcg cggaaaaacc aggtcattat
    88141 gcaggcctgc tgcgacgtgc tgaatcgccc gctgcaaatt gttgcctctg accagtgctg
    88201 tgcgctcggt gcggcgattt ttgctgccgt cgccgcgaaa gtgcacgcag acatcccatc
    88261 agcccagcaa aaaatggcca gtgcggtaga gaaaaccctg caaccgcgca gcgaacaggc
    88321 acaacgcttt gaacagcttt atcgccgcta tcagcaatgg gcgatgagcg ccgaacaaca
    88381 ctatcttcca acttccgccc cggcacaggc tgcccaggcc gttgcgactc tataaggaca
    88441 cgataatgac gatttttgat aattatgaag tgtggtttgt cattggcagc cagcatctgt
    88501 atggcccgga aaccctgcgt caggtcaccc aacatgccga gctcgttgtt aatgcgctga
    88561 atacggaagc gaaactgccc tgcaaactgg tgttgaaacc gctgggcacc acgccggatg
    88621 aaatcaccgc tatttgccgc gacgcgaatt acgacgatcg ttgcgctggt ctggtggtgt
    88681 ggctgcacac cttctccccg gccaaaatgt ggatcaacgg cctgaccatg ctcaacaaac
    88741 cgttgctgca attccacacc cagttcaacg cggcgctgcc gtgggacagt atcgatatgg
    88801 actttatgaa cctgaaccag actgcacatg gcggtcgcga gttcggcttc attggcgcgc
    88861 gtatgcgtca gcaacatgcc gtcgttaccg gtcactggca ggataaacaa gcccatgagc
    88921 gtatcggctc ctggatgcgt caggcggttt ctaaacagga tacccgtcat ctgaaagtct
    88981 gccgttttgg cgataacatg cgtgaagtgg cggtcaccga tggtgataaa gttgccgcac
    89041 agatcaagtt cggtttctcc gtcaatacct gggcggttgg cgatctggtg caggtggtga
    89101 actccatcag cgacggcgat gttaacgcgc tggtcgatga gtacgaaagc tgctacacca
    89161 tgacgcctgc aacacaaatc cacggcgaaa aacgacagaa cgtgctggaa gcggcgcgta
    89221 ttgagctggg gatgaagcgt ttcctggaac aaggtggctt ccacgcgttc accaccacct
    89281 ttgaagattt gcacggtctg aaacagcttc caggtctggc cgtacagcgt ctgatgcagc
    89341 agggttacgg ctttgcgggc gaaggcgact ggaaaaccgc cgccctgctt cgcatcatga
    89401 aggtgatgtc aaccggtctg cagggcggca cctcctttat ggaggactac acctatcact
    89461 tcgagaaagg taatgacctg gtgctcggct cccatatgct ggaagtctgc ccgtcgattg
    89521 ccgtagaaga gaaaccgatc ctcgacgttc agcatctcgg tattggtggt aaggacgatc
    89581 ctgcccgact gatcttcaat acccaaaccg gtccagcgat tgtcgccagc ctgattgatc
    89641 tcggcgatcg ttaccgtctg ctggttaact gtatcgacac ggtgaaaaca ccgcactccc
    89701 tgccgaaact gccggtggcg aatgcgctgt ggaaagcgca accggatctg ccaactgctt
    89761 ccgaagcgtg gatcctcgct ggtggcgcgc accataccgt cttcagccat gcgctgaacc
    89821 tcaacgatat gcgccagttc gccgagatgc acgacattga aatcacggtg attgataacg
    89881 acacccgcct gccagcgttt aaagacgcac tgcgctggaa cgaagtgtat tacggatttc
    89941 gtcgctaagt agccgcatca ggtatgtaac gcctgatgcg atgctggcgc gtcttatcag
    90001 gcctacacgt tgcgattttg taggccggat aagcaaaccg catccggcat tcaacgcctg
    90061 atgcgacgct ggcgcgtctt atcaggccta cgcgttccga ttttgtaggc cggataagca
    90121 aagcgcatcc ggcattcaac gcctgatgcg acgctggcgc gtcttatcag gcctacacgc
    90181 tgcgattttg taggccggat aagcaaagcg catccggcac gaaggagtca acatgttaga
    90241 agatctcaaa cgccaggtat tagaagccaa cctggcgctg ccaaaacaca acctggtcac
    90301 gctcacatgg ggcaacgtca gcgccgttga tcgcgagcgc ggcgtctttg tgatcaaacc
    90361 ttccggcgtc gattacagcg tcatgaccgc tgacgatatg gtcgtggtta gcatcgaaac
    90421 cggtgaagtg gttgaaggta cgaaaaagcc ctcctccgac acgccaactc accggctgct
    90481 ctatcaggca ttcccctcca ttggcggcat tgtgcatacg cactcgcgcc acgccaccat
    90541 ctgggcgcag gcgggtcagt cgattccagc aaccggcacc acccacgccg actatttcta
    90601 cggcaccatt ccctgcaccc gcaaaatgac cgacgcagaa atcaacggcg aatatgagtg
    90661 ggaaaccggt aacgtcatcg tagaaacctt tgaaaaacag ggtatcgatg cagcgcaaat
    90721 gcccggcgtt ctggtccatt cccacggccc gtttgcatgg ggcaaaaatg ccgaagatgc
    90781 ggtgcataac gccatcgtgc tggaagaggt cgcttatatg gggatattct gccgtcagtt
    90841 agcgccgcag ttaccggata tgcagcaaac gctgctggat aaacactatc tgcgtaagca
    90901 tggcgcgaag gcatattacg ggcagtaatg actgtataaa accacagcca atcaaacgaa
    90961 accaggctat actcaagcct ggttttttga tggattttca gcgtggcgca ggcaggtttt
    91021 atcttaaccc gacactggcg ggacaccccg caagggacag aagtctcctt ctggctggcg
    91081 acggacaacg ggccgttgca ggttacgctt gcaccgcaag agtccgtggc gtttattccc
    91141 gccgatcagg ttccccgcgc tcagcatatt ttgcagggtg aacaaggctt tcgcctgaca
    91201 ccgctggcgt taaaggattt tcaccgccag ccggtgtatg gcctttactg tcgcgcccat
    91261 cgccaattga tgaattacga aaagcgcctg cgtgaaggtg gcgttaccgt ctacgaggcc
    91321 gatgtgcgtc cgccagaacg ctatctgatg gagcggttta tcacctcacc ggtgtgggtc
    91381 gagggtgata tgcacaatgg cactatcgtt aatgcccgtc tgaaaccgca tcccgactat
    91441 cgtccgccgc tcaagtgggt ttctatagat attgaaacca cccgccacgg tgagctgtac
    91501 tgcatcggcc tggaaggctg cgggcagcgc atcgtttata tgctggggcc ggagaatggc
    91561 gacgcctcct cgcttgattt cgaactggaa tacgtcgcca gccgcccgca gttgctggaa
    91621 aaactcaacg cctggtttgc caactacgat cctgatgtga tcatcggttg gaacgtggtg
    91681 cagttcgatc tgcgaatgct gcaaaaacat gccgagcgtt accgtcttcc gctgcgtctt
    91741 gggcgcgata atagcgagct ggagtggcgc gagcacggct ttaaaaacgg cgtctttttt
    91801 gcccaggcta aaggtcggct aattatcgac ggtatcgagg cgctgaaatc cgcgttctgg
    91861 aatttctctt cattctcgct ggaaactgtc gctcaggagc tattaggcga aggaaaatct
    91921 atcgataacc cgtgggatcg aatggacgaa attgaccgcc gtttcgccga agataaacct
    91981 gcgctggcaa cttataacct gaaagattgc gagctggtga cgcagatctt ccacaaaact
    92041 gaaatcatgc catttttact cgaacgggca acggtgaacg gcctgccggt ggaccgacac
    92101 ggcggttcgg tggcggcatt tggtcatctc tattttccgc gaatgcatcg cgctggttat
    92161 gtcgcgccta atctcggcga agtgccgccg cacgccagcc ctggcggcta cgtgatggat
    92221 tcacggccag ggctttatga ttcagtgctg gtgctggact ataaaagcct gtacccgtcg
    92281 atcatccgca cctttctgat tgatcccgtc gggctggtgg gaggcatggc gcagcctgat
    92341 ccagagcaca gtaccgaagg ttttctcgat gcctggttct cgcgagaaaa acattgcctg
    92401 ccggagattg tgactaacat ctggcacggg cgcgatgaag ccaaacgcca gggtaacaaa
    92461 ccgctgtcgc aggcgctgaa aatcatcatg aatgcctttt atggcgtgct cggcaccacc
    92521 gcctgccgct tcttcgatcc gcggctggca tcgtcgatca ccatgcgtgg tcatcagatc
    92581 atgcggcaaa ccaaagcgtt gattgaagca cagggctacg acgttatcta cggcgatacc
    92641 gactcaacgt ttgtctggct gaaaggcgca cattcggaag aagaagcggc gaaaatcggt
    92701 cgtgcactgg tgcagcacgt taacgcctgg tgggcggaaa cgctgcaaaa acaacggctg
    92761 accagcgcat tagaactgga gtatgaaacc catttctgcc gttttctgat gccaaccatt
    92821 cgcggagccg ataccggcag taaaaagcgt tatgccggac tgattcaggt gggcgacaag
    92881 cagcggatgg tgtttaaagg gctggaaacc gtgcgcaccg actggacgcc gctggcccag
    92941 cagtttcagc aggagctata cctgcgcatc ttccgcaacg agccatatca ggaatatgta
    93001 cgcgaaacca tcgacaaact gatggcgggt gaactggatg cgcgactggt ttaccgtaaa
    93061 cgccttcgcc gtccgctgag cgagtatcag cgtaatgtgc cgcctcatgt acgcgccgct
    93121 cgccttgccg atgaagaaaa ccaaaagcgt ggtcgcccct tgcaatatca gaatcgcggc
    93181 accattaagt acgtatggac caccaacggc ccggagccgc tggactacca acgttcacca
    93241 ctggattacg aacactatct gacccgccag ctacaacccg tggcggaggg aatactccct
    93301 tttattgagg ataattttgc tacacttatg accgggcaac ttgggctatt ttgagcaaaa
    93361 aaaagagttc gccagatacc attttgatgc gtgacgaatg ctttgccatc cagtaccata
    93421 gcgccctttc cattcctgga cctgaataac accactacct cataagcacg gtagcgggtg
    93481 gttattgcct gcaattaaag atatagagcc gaacacatat gccttttaca cttggtcaac
    93541 gctggatcag cgatacagaa agcgaattgg gacttggaac cgttgtcgcg gtggatgcgc
    93601 gaactgtcac tttacttttc ccatctactg gtgaaaaccg tctgtacgca cgcagtgatt
    93661 cccccgtgac ccgcgtgatg ttcaaccctg gtgataccat taccagccat gacggctggc
    93721 agatgcaagt cgaagaagta aaagaagaaa atggcttgct gacctatatc ggtactcgcc
    93781 tggatactga agagtccggc gtagccctgc gtgaagtttt ccttgatagc aaactggtgt
    93841 tcagcaaacc gcaggaccgt ctgtttgccg ggcagattga ccgtatggac cgctttgcgc
    93901 tgcgttatcg cgcgcgtaaa tattccagcg aacagttccg tatgccgtac agcggcctgc
    93961 gcggtcagcg taccagcctg atcccgcatc agctcaacat cgctcatgat gttggtcgcc
    94021 gccacgcgcc gcgcgtcctg ctggctgacg aagtgggttt agggaaaacc attgaagccg
    94081 ggatgatcct gcatcagcaa ctgctctctg gcgctgctga acgtgtgcta attatcgtcc
    94141 cggaaacctt acagcatcag tggctggtag aaatgctgcg ccgtttcaac ctgcgctttg
    94201 cgctatttga tgatgagcgt tatgccgaag ctcagcacga tgcttacaac ccgtttgaca
    94261 ccgaacagct ggtgatttgc tcgctggatt ttgcccgtcg tagcaaacag cgcctggaac
    94321 atctctgtga agccgaatgg gacctgctgg tggtcgatga agcgcatcac ctggtgtgga
    94381 gcgaagatgc gccaagccgt gaatatcagg ccattgaaca actggcagag cacgtgccgg
    94441 gcgttctgct gctgaccgcg accccggaac agctggggat ggaaagccac ttcgcccgtc
    94501 tgcgtctgct ggacccgaac cgtttccacg attttgcgca gttcgttgaa gagcagaaaa
    94561 attatcgtcc ggttgcggac gccgttgcca tgctgctggc aggtaacaaa ctgagcaatg
    94621 acgaactgaa catgctcggc gagatgatcg gcgagcagga tatcgagccg ctgttgcagg
    94681 cagcaaacag cgacagcgaa gatgcccaga gcgcccgtca ggagctggtt tcgatgctga
    94741 tggatcgcca cggcaccagc cgcgtgctgt tccgtaacac gcgtaacggt gtgaaaggat
    94801 tcccgaaacg cgagctgcac accattaagc tgccgctacc gacgcagtat cagacggcta
    94861 ttaaagtctc cggcattatg ggcgcacgta aaagtgcgga agatcgtgct cgcgatatgc
    94921 tctacccgga gcgtatttat caggaatttg aaggtgataa cgccacctgg tggaacttcg
    94981 atccgcgcgt tgagtggctg atgggctacc tgaccagcca tcgctctcag aaagtgctgg
    95041 tgatctgcgc caaagctgcc actgcgctgc aactggagca ggtactgcgc gaacgtgaag
    95101 gtattcgcgc tgcggtgttc cacgaaggta tgtcgattat cgaacgtgac cgcgctgccg
    95161 cctggtttgc cgaagaagac accggcgcac aggtactgct gtgctcagaa atcggttctg
    95221 aaggacgtaa cttccagttc gccagccaca tggtgatgtt tgacctgcca ttcaacccgg
    95281 atctactgga gcagcgtatt ggtcgtctgg atcgtatcgg ccaggcgcac gatattcaga
    95341 tccatgtgcc ttatctggag aaaaccgctc agtcggtgct ggtgcgctgg tatcacgaag
    95401 gtctggatgc atttgagcac acctgcccga ccggacgcac tatttacgat agcgtataca
    95461 acgatctgat taactatctg gcttcaccgg atcaaaccga aggctttgac gatctgatca
    95521 aaaactgccg cgagcaacat gaagcgctga aagcacagct ggaacagggt cgtgaccgcc
    95581 tgctggaaat ccactccaac ggtggcgaaa aagcccaggc actggcagaa agcattgaag
    95641 agcaggatga cgataccaac ctgatcgcct tcgccatgaa cctgttcgat attatcggta
    95701 tcaatcagga cgatcgcggc gacaacatga tcgtgctgac gccgtccgat catatgctgg
    95761 tgccggactt ccctggcctg tcggaagatg gcatcaccat cacctttgat cgtgaagtgg
    95821 cgctggcgcg tgaagatgca cagtttatta cctgggagca tccgctgatc cgcaacggtc
    95881 tggatctgat cctttctggc gataccggta gcagcacgat ttcactgtta aaaaacaaag
    95941 cgttgccggt aggtacgctg ttggtggaac tgatttatgt ggttgaagcc caggctccga
    96001 agcagttgca gctcaaccgc ttcctgccac cgacgccggt acgtatgctg ctggataaaa
    96061 acggcaacaa cctggcggcg caggtagagt ttgaaacctt taaccgccag cttaacgcgg
    96121 ttaaccgtca caccggcagc aaactggtta acgccgtgca gcaggatgtt cacgctatcc
    96181 ttcaactggg tgaagcgcag atcgagaaat ctgcccgtgc attgattgat gcagcgcgta
    96241 acgaagccga cgaaaaactg tctgccgagc tgtctcgtct ggaagctctg cgtgcagtga
    96301 acccgaacat tcgtgacgac gaactgaccg ccattgagag caaccgtcag caggtaatgg
    96361 aaagcctgga tcaggcaggt tggcgtctgg atgccctgcg tttgatcgtt gtaacgcatc
    96421 agtaacggag ccgaaaatgg ggatggaaaa ctacaatcca ccgcaggaac cctggttggt
    96481 tatcctgtat caggatgacc atattatggt ggtcaacaag ccgagcggtt tgttgtcagt
    96541 gccgggtcgt ctggaagagc acaaagacag cgtgatgacg cgcattcagc gtgattatcc
    96601 gcaggcagaa tcggtgcatc gtctggatat ggctaccagc ggcgtgattg tagtggcgct
    96661 gaccaaagcc gcggagcggg agttaaaacg ccagttccgc gagcgcgagc cgaaaaagca
    96721 gtatgtggcc cgcgtctggg ggcatccatc ccccgcagaa ggtctggtgg atctgccgct
    96781 gatttgcgac tggccaaacc gcccgaaaca gaaagtctgt tacgaaacgg gtaaacctgc
    96841 gcagacggaa tatgaagtgg tggagtatgc ggcggataac acggcaagag tggtgttaaa
    96901 accgattacc gggcgttcgc atcaattgcg tgtgcatatg ctggcgctgg gtcatccgat
    96961 tctcggcgat cgtttttatg catcaccaga agcgagagcg atggcaccgc gtttgttgct
    97021 acatgcagag atgttgacga ttacccatcc ggcgtatggc aatagtatga cgtttaaagc
    97081 gccagcggat ttttaatcgt cacgctggcg cagttcggta ggcggataag gcgttcacgc
    97141 cgcatccggc aattgtgcgc cgattgcctg atgcgacgcg ggccgcgtct tatcaggcct
    97201 acagtttaaa acttatttaa accctttctg ctgctttatc agctcatatg cctgctgaat
    97261 ttcctgcgct ttctgcttcg ccatctccat catctcaggc ggcaaacctt tcgccaccag
    97321 cttatcggga tggtgttcac tcatcagctt acggtaggca cgtttgatgg tggtcgcatc
    97381 atccgtcggc ttcacgccca gcacattaca ggcatcttcc agcgttgggc cacgctgcgc
    97441 ttgctgccag ttaccaccgc cagtttgctg ctgataaccg ccgccaaact gtgcaccgcc
    97501 ctgcatcatg cgcaaaaact ggtcaaactg agcgcgggag atccctaatt cttctgcaat
    97561 gacatacagc accgcccgtt cattcgggtg cagtgaacca tcagcaaacg ccgcctgaat
    97621 ctggatctcc agaaacatac gaattaagtc aaaacgacca aagcagacac tgcgaaactg
    97681 gcgcatcttt tcgcgcagcg ggtaattgtc tgattttccc acccggaacg cattttgcgc
    97741 cgcagtacgg gaagcgccat gaagattcat tcggtccatc aactggctgg cgatatgaat
    97801 atcagcctcc gtgacgcgac ctttggattt ggttaaatgc cccatcactt caaaagtggt
    97861 ggcaaaaaac agcgcctgac gctcacgctg gttggcgaac cacgccattt tacggctacg
    97921 ggctttatca aacatatggc caattaacag gcctaacact acgccccaaa agccgccgcc
    97981 catcagtaag gccacggcca cgccaatgat ttttccccaa tactgcatat attccccaaa
    98041 tcgacacacg gatatcaggg ctatctccca caatataaag gtgcttttac cgttttccgg
    98101 ctgcggtcaa ttgtgggaca tcgcctataa tttgcattat catacctgtc attcaatgcc
    98161 gtgcctaaca ccactgacgc tattcggaca ggattaacac tagcgtagag atgacgagta
    98221 cgttagtctc tgaccgtttg tcacgcgcaa cgttaccgat gatggaacaa taaaatcaac
    98281 gtatgaaaaa acgtatcccc actctcctgg ccaccatgat tgccaccgcc ctttatagtc
    98341 aacagggact ggcagccgac ctcgcctcac agtgcatgtt gggcgtgcca agctatgacc
    98401 gtcctctggt acagggcgat accaatgact tacccgtgac tatcaatgct gaccacgcga
    98461 aaggggacta cccggatgac gccgtgttta ctggcagcgt ggatatcatg cagggtaaca
    98521 gccgtctgca ggccgacgaa gtgcagctcc atcaaaaaga ggcaccagga caaccggagc
    98581 cggtacgtac cgttgatgcg ctcggtaatg tccattacga cgataaccag gtgatcctca
    98641 aagggccgaa aggctgggcg aatctgaaca ccaaagatac caacgtctgg gaaggtgatt
    98701 accagatggt gggtcgccag ggtcgcggta aagcggacct gatgaaacaa cgtggcgaaa
    98761 accgctatac cattctggat aacggtagct ttacctcctg tctgccgggt tctgacacct
    98821 ggagcgtggt aggtagcgaa attattcatg accgcgaaga acaagttgcg gagatctgga
    98881 acgcccgctt taaggtgggt ccggtaccga tcttttatag cccctatttg cagttgccgg
    98941 tgggtgacaa acgtcgctct ggtttcttga tcccgaacgc caagtacacc accaccaact
    99001 actttgagtt ctacctgcca tattactgga acatcgcgcc aaatatggat gccaccatca
    99061 cgccgcatta tatgcatcgt cgtggcaaca tcatgtggga gaacgaattc cgctacctct
    99121 cccaggcggg cgctggcttg atggaactgg actatctgcc ttcagataaa gtctatgaag
    99181 atgaacaccc gaacgatgac agttcacgtc gttggttatt ctactggaac cactccgggg
    99241 tcatggatca ggtgtggcgt ttcaacgtcg actacaccaa ggtcagcgat cctagctact
    99301 tcaatgattt cgataacaag tacggttcca gtactgacgg ctacgcaacg caaaaattca
    99361 gcgttggcta tgcggtgcaa aacttcaatg ccaccgtttc aaccaagcag ttccaggttt
    99421 tcagcgaaca gaacaccagt agctactcgg cagagccgca gttagacgtt aattactacc
    99481 agaatgatgt tggtccgttt gatacgcgta tttacggcca ggcagtgcac tttgttaaca
    99541 ccagagacga catgcctgaa gcaacccgtg ttcacctgga accgaccatc aatttgccgc
    99601 tctctaataa ctggggcagc atcaataccg aagcgaagtt gctggcaacc cattatcagc
    99661 aaactaatct tgactggtat aactccagaa acacgaccaa gctggacgaa tccgttaacc
    99721 gcgtaatgcc gcaattcaaa gttgacggca aaatggtctt tgaacgcgat atggaaatgc
    99781 tggctccggg ttatacccaa acgctggaac cgcgcgcgca gtatttgtac gtgccgtatc
    99841 gcgatcagag cgacatctat aactacgact catctctgct gcaatctgac tactctggcc
    99901 tgttccggga ccggacttac ggcggtcttg accgtattgc ctccgctaac caggtgacga
    99961 ccggtgtcac atctcgcata tatgatgatg ctgccgttga acgttttaat atttccgttg
   100021 gtcaaatcta ctatttcacg gagtctcgca ctggcgatga caacataaca tgggagaatg
   100081 acgacaaaac gggttcactg gtgtgggcag gcgatactta ctggcgtatc tccgagcgtt
   100141 ggggattgcg tggcgggatt cagtacgata cacgtctgga taacgtagcg accagtaact
   100201 ccagcattga ataccgtcgg gatgaagacc gtctggtaca gctgaattac cgttacgcca
   100261 gcccggaata tattcaggct acgctgccta agtactattc cactgctgag caatataaga
   100321 atggtatttc gcaggtaggt gctgtcgcca gctggccaat tgccgatcgt tggtccattg
   100381 ttggggccta ctactacgac accaatgcta acaagcaagc cgactctatg ttaggtgtgc
   100441 aatacagctc ctgctgctat gcaattcgcg tcggttacga gcggaagctg aacggttggg
   100501 ataacgataa acaacatgcg gtatatgaca acgcaatcgg ctttaacatc gaacttcgcg
   100561 gcctgagctc caactacggt ctgggtacgc aagagatgct gcgttcgaac attctgccgt
   100621 atcaaaacac tttgtgattt gttgatttac cacgtaatcc gcagtgcggt taattgaaat
   100681 ggaaaaagta tgaagaactg gaaaacgctg cttctcggta tcgccatgat cgcgaatacc
   100741 agtttcgctg ccccccaggt agtcgataaa gtcgcagccg tcgtcaataa cggcgtcgtg
   100801 ctggaaagcg acgttgatgg attaatgcag tcggtaaaac tgaacgctgc tcaggcaagg
   100861 cagcaacttc ctgatgacgc gacgctgcgc caccaaatca tggaacgttt gatcatggat
   100921 caaatcatcc tgcagatggg gcagaaaatg ggagtgaaaa tctccgatga gcagctggat
   100981 caggcgattg ctaacatcgc gaaacagaac aacatgacgc tggatcagat gcgcagccgt
   101041 ctggcttacg atggactgaa ctacaacacc tatcgtaacc agatccgcaa agagatgatt
   101101 atctctgaag tgcgtaacaa cgaggtgcgt cgtcgcatca ccatcctgcc gcaggaagtc
   101161 gaatccctgg cgcagcaggt gggtaaccaa aacgacgcca gcactgagct gaacctgagc
   101221 cacatcctga tcccgctgcc ggaaaacccg acctctgatc aggtgaacga agcggaaagc
   101281 caggcgcgcg ccattgtcga tcaggcgcgt aacggcgctg atttcggtaa gctggcgatt
   101341 gctcattctg ccgaccagca ggcgctgaac ggcggccaga tgggctgggg ccgtattcag
   101401 gagttgcccg ggatcttcgc ccaggcatta agcaccgcga agaaaggcga cattgttggc
   101461 ccgattcgtt ccggcgttgg cttccatatt ctgaaagtta acgacctgcg cggcgaaagc
   101521 aaaaatatct cggtgaccga agttcatgct cgccatattc tgctgaaacc gtcgccgatc
   101581 atgactgacg aacaggcccg tgtgaaactg gaacagattg ctgctgatat caagagtggt
   101641 aaaacgactt ttgctgccgc agcgaaagag ttctctcagg atccaggctc tgctaaccag
   101701 ggcggcgatc tcggctgggc tacaccagat attttcgatc cggccttccg tgacgccctg
   101761 actcgcctga acaaaggtca aatgagtgca ccggttcact cttcattcgg ctggcattta
   101821 atcgaactgc tggatacccg taatgtcgat aaaaccgacg ctgcgcagaa agatcgtgca
   101881 taccgcatgc tgatgaaccg taagttctcg gaagaagcag caagctggat gcaggaacaa
   101941 cgtgccagcg cctacgttaa aatcctgagc aactaatggt taaaacccaa cgtgttgtga
   102001 tcactcccgg cgagcccgcc gggattggcc cggacttagt tgtccagctt gcacagcgtg
   102061 agtggccggt cgaactggtt gtttgtgccg atgccactct ccttaccgac cgggcagcga
   102121 tgctcggttt gccgctcacc ctccgccctt attcccccaa ctcccctgca caaccgcaaa
   102181 ctacgggcac attaacgcta cttcctgtcg cgctacgtga atctgtcact gcggggcagt
   102241 tagcgattga aaatgggcat tacgtggtgg agacgctggc gcgagcgtgt gatggctgtc
   102301 tgaacggtga atttgctgcg ctgatcacag gccccgtgca taaaggcgtc attaacgacg
   102361 caggcattcc gtttaccggt cataccgagt ttttcgaaga gcgttcgcag gcgaaaaaag
   102421 tggtgatgat gctggcgacc gaagaacttc gcgtggcgct ggcaacgacg catttaccgc
   102481 tgcgcgatat cgcagatgct atcacccctg cacttttgca cgaagtgatt gctattttgc
   102541 atcacgattt gcgtacaaaa tttggtattg ccgaaccgcg cattctggtc tgcgggctga
   102601 atccgcacgc gggcgaaggc ggtcatatgg gtacggaaga gatagacacc attattccgg
   102661 tgctcgacga gctgcgggcg caggggatga aactcaacgg gccgctgcct gccgataccc
   102721 tgtttcagcc gaaatatctc gataacgccg acgccgtgct ggcgatgtac cacgatcagg
   102781 gtcttcccgt gctaaaatac cagggcttcg ggcgcggtgt gaacattacg ctgggcctgc
   102841 cctttattcg cacatcagtg gaccacggca ccgcgcttga actggcggga cgtggcgaag
   102901 ccgatgtcgg cagttttatt acggcgctta atctcgccat caaaatgatt gttaacaccc
   102961 aatgaataat cgagtccacc agggccactt agcccgtaaa cgcttcgggc aaaactttct
   103021 caacgatcag ttcgtgatcg acagcattgt ttctgccatt aacccgcaaa agggccaggc
   103081 aatggtcgaa atcggccccg gtctggcggc attgaccgag ccggtcggcg aacgtctgga
   103141 ccagctgacg gtcatcgaac ttgaccgcga tctggcggca cgtctgcaaa cgcatccatt
   103201 cttaggcccg aaactgacga tttatcagca ggatgcgatg acctttaact ttggcgaact
   103261 ggccgagaaa atgggtcagc cgctgcgtgt tttcggcaac ctgccttata acatctccac
   103321 gccgttgatg ttccatctgt ttagctatac tgatgccatt gccgacatgc actttatgtt
   103381 gcaaaaagag gtggtgaatc gtctggttgc aggaccgaac agcaaagcgt atggtcgatt
   103441 aagcgttatg gcgcaatact attgcaacgt gatcccagtg ctggaagtac cgccgtcagc
   103501 ctttacacca ccacccaaag tggattccgc cgtcgtgcgc ctggttcctc atgcaacgat
   103561 gcctcacccg gttaaagatg ttcgtgtgtt gagccgcatc accaccgaag cctttaacca
   103621 gcgtcgtaaa accatccgta acagcctcgg caacctgttt agcgtcgagg tgttaacggg
   103681 aatggggatc gacccggcga tgcgagcgga aaatatctct gtcgcgcaat attgccagat
   103741 ggcgaactat ctggcggaga acgcgccttt gcaggagagt taacgatgat caattcgccc
   103801 cgagtgtgta ttcaggttca aagcgtctac attgaggcac aatcttcacc tgataatgaa
   103861 cgttacgttt ttgcttatac cgtaaccata cgcaatctgg ggcgagcgcc agtgcagttg
   103921 ttggggcgtt actggctgat caccaatggc aatggccgtg aaaccgaagt ccagggcgaa
   103981 ggagtggttg gcgtccagcc acttatcgcg cctggcgaag agtaccagta caccagcggt
   104041 gcaatcattg aaaccccgct gggcaccatg cagggtcact acgaaatgat cgatgaaaat
   104101 ggcgtccctt tcagcatcga cattcccgta ttccgactcg ccgttcccac actcattcat
   104161 taaaagaata tggcgacata ccttattggc gacgttcatg gttgttacga tgaactgatc
   104221 gcattgctgc ataaagtaga atttacccct gggaaagata ccctctggct aacgggcgat
   104281 ctggtcgcgc gcggcccggg ttcgctggat gttctgcgct atgtgaaatc cttaggcgac
   104341 agcgtacgtc tggtgctggg caatcacgat ctgcatctgc tggcggtatt tgccgggatc
   104401 agccgcaata aaccgaaaga tcgcctgaca ccgctgctgg aagcgccgga tgccgacgag
   104461 ctgcttaact ggctgcgtcg ccagcctttg ctgcaaatcg acgaagagaa aaagttggtg
   104521 atggcccacg cagggatcac gccgcagtgg gatctacaga ccgccaaaga gtgcgcgcgc
   104581 gatgtagaag cggtgctatc gagtgactcc tatcccttct ttctcgacgc tatgtacggc
   104641 gatatgccaa ataactggtc accggaattg cgggggctgg gaagactgcg ttttatcacc
   104701 aacgctttta cccgtatgcg tttttgcttc ccgaacggtc aactggatat gtacagcaaa
   104761 gaatcgccgg aagaggcccc tgccccactg aaaccgtggt ttgcgattcc tggccctgtc
   104821 gctgaagaat acagcatcgc ctttggtcac tgggcatcgc tggagggcaa aggtacgccg
   104881 gaaggtatat acgcgctgga taccggctgc tgctggggtg gtacattaac ctgcctgcgc
   104941 tgggaagata aacagtattt tgtccagccg tcgaaccggc ataaggattt gggcgaagcg
   105001 gcggcgtctt aaacacagcc tgatatagga aggccggata agacgcgacc ggcgtcgcat
   105061 ccggcactag ccgtaaattc tatacaaaat taccgccgct ccagaatctc aaagcaatag
   105121 ctgtgagagt tctgcgcatc agcatcgtgg aattcgctga ataccgattc ccagtcatcc
   105181 ggctcgtaat ccgggaaatg ggtgtcgcct tccacttctg cgtcgatatg cgtcagatac
   105241 agtttttgcg cttttggcaa gaactgttca taaacgcgac cgccgccaat caccatgatt
   105301 tctggtacgt caccacacgc cgcgatggct tcatccaccg acttcaccca cgttacgcga
   105361 tcgtccgtac ccggttgact gctgaggata atatttttgc gtcctggcaa cggacgaccg
   105421 attgattccc aggtatggcg gcccataatc acgggtttat ttaaggtgtt gcgtttaaac
   105481 caggcgagat cggcaggcag gttccacggc atggcgtttt ccatgccgat aacgcgatct
   105541 accgctaacg ccgcaatcag actgatcatt gagatttccc gataaaaaaa attgtcgcca
   105601 ctatacgtaa agcgtaaacc gtcgtcgact ggtgcgagga tgatgttgag gaaaatttta
   105661 tattctgctg gcgagtccac gctctctccc tggactcgcc gcattacaat gaaacaaaaa
   105721 caaacagtta gctgtaaagt gtgatttacg tcactcttta ttaggatgag ggtttcgttt
   105781 ccggttcatc cgccatgttg ccggtatgtt taccttcttc ggttccctgc cagccatgtc
   105841 gttgaattaa tgacagatgt tcgcggtcct cggtaatgat ctcacttaac atcgcgctgg
   105901 tgcgtttata gaccgccgcg cgggctttgg tgtcgttctc aaccattgcc atctcttcca
   105961 ccatctgaat attaaagcgg cggaacacat cggcacgttc tcgcgcttca tacggcccca
   106021 gacctaaact ttccagtgcc agacgcccgg ttttcagcgc accttcgaag gtttcacgct
   106081 ccggcttttc aacgcctgcc tgacgcaaac gaatgtagtg gtcgacatcg cgggcgcggg
   106141 caataatctg caaatgcggg aaatgttctt tcaccatctc tgtcagttgc aggttggttt
   106201 gcggatcgtc gatggcgtta atcagcactt ccgctttcgc cgctccggca gattccagta
   106261 aatccatccg cgtggcatcg ccataaaaca ctttcatacc aaatttacgc aaggtttcga
   106321 tatggtccgg atcgtgatcg agtaccacca ttttcacccc gctggagagc agtaaacgtc
   106381 cggtaatctg cccaaaacga ccgaatccgg cgataatcac gcgcggctgt tcttcgtcga
   106441 tctcatcggc ttcacgcgct ttctcagtag aagattgctc aaggcgattg aggatcacca
   106501 gcagaatcgg cgttgctgcc atcgacagcg ccaccgccag ggtcagcgat ttcgcccact
   106561 ccggctccag cacattcgcc atctgcgccg cgccaaatac cacaaaggca aactcactgc
   106621 cctgccctaa caacaccgca aaccaacgac gctgtttatt tggcacttgc aacggtcggg
   106681 caatcagcca cagcatggcg attttgatga tgaggaaacc gagcagcaaa atgacaatgc
   106741 gcaatgggtt ttcaagcagc gtgccaaagt ctatcgacat gccaacaccg atgaaaaaca
   106801 gccccaacag caaaccttta aatggttcga tatcgctctc cagcgcatga cggtattccg
   106861 agcttgtcag cagtacgccc gccagaaacg cgcccatcgc catcgacaag ccgacctctt
   106921 ccagcagcaa accaaagcca aacacgagga ataacgccac ggcactaaac acttcccgca
   106981 agccagagcg ggctacaaaa cgcagcgccg gacgcgtgac atagcgcccc agcaatacca
   107041 ccagcaccag cgcacccgcc acttttaacg ccgagagagc aaatgcgccc atcgtcgtcg
   107101 aggcactgct cgttgccagt agcggaatca tcgccaccag cgggatcgcc gcgatatcct
   107161 ggaacagcag caccgcaaag gcactgcgac ccatttgcgt caccatcaga ttgcgttcat
   107221 tcatcgcctg catggcaatc gccgtagagg agagcgccag cgtcatgccg atcaattccg
   107281 cgacctgcca gcgcagccca agtaacatgc agaacagccc cagcaggccg ccgcaaatca
   107341 ccatctgcaa tgcgccaccg ccgaacactg ccgcacgcag cttccacagc ctttgtggat
   107401 cgagttcgag gccgataata aacagcatca gcaccacccc aatctcggca aagtgcagaa
   107461 tagattcggc atcggtcacc agtcgcagcc cccacgggcc aataatgcag ccggcgatca
   107521 ggtagccaag taccgatccc agaccaagac gtaccgcaat gggtacaatc agcgctgccg
   107581 aaccgagata aatcagcgcc tgaatcagcg tatggctatc catgatgggc ctcctgccat
   107641 tccagcagac gttgcttata gtgacgcgcc tgcccttcga gggtttcgtc gtcacaaata
   107701 aaggtgcagt gcatggcaaa cggtggcagc cagttcagcc cgcagtagat tgccgtcgcc
   107761 tgtagcggct gcgacagcac atcaaagccc ggatgcgcac caatttcaaa atggctttcc
   107821 ccgccgccgg tcgtcaccgc ccacagcaaa tgtttgccat gcagcgcctt gccgccgtga
   107881 ccgtaagccc agccgtgcga caaaacttta tcgatccaaa gtttaagaag cggaggaatg
   107941 ctgtaccact gcatcggatg ctgccagacg atcagatcgg cgcgagacag cgcctcctgc
   108001 tcggcggcaa tatcgatatt gaagtcagga tagagttgat aaagagagcg aatttcgacg
   108061 ccttccagcg tccttgcctg ttcaagcatc cgtttattcg catgggaatg atgcggatac
   108121 ggatgcgcat aaattataag aatcataggt agcccgcgtg attacgcctt tgttttccca
   108181 caaagtgtag tcatatattc acgcggctta tagtcaataa tattgattca gaaaagagag
   108241 aaatctgaac aatttacccc attctctgcc cgcggatcgt catattgctg gtctgcgcca
   108301 gtgacatccc tcgatgtctc cggggcaaac gctacggaaa tcaacaagcc aaacagcgag
   108361 atacccgccc ccattagcat cgtgttactg ataccgtaat tattgataaa gatcggtagt
   108421 gcccaggtcg aaacaatggt gccaatacga cttaaagaca taatcacgcc cacggcagag
   108481 gcgcggatat ctgtcgggaa gagttcatta ggatagagcc actgcaaatt acccggcccg
   108541 ccagagaaaa aggcatacac cgcaaaggcc atcactacca gccagatccc catatccggg
   108601 atcagcccca aaaccgccag cgccagcgtc atcatggcaa agctgccaat caacaatgga
   108661 cgccgtccgg cagtgtttag ccacagcatc ggcggaatac agccgagcat aaagaacagg
   108721 ctaatcacca cgtttcccag cgccgcgttt ttgccaaccc ccaatcccaa caaaccaacg
   108781 atttgcgggc caaaggtgta aatggcgaac attgggatca cctggcaggt ccagatggcg
   108841 gcaacaaaca gaacaaaagg aaaatggcgg cgattaaaca gatcgcgaaa acgggtttgc
   108901 tgcggctgct cttcatcgaa agccaccggt tcgccaaaca gtttgatcat catctcttcg
   108961 cactctttta ctcgcccttt gcgtaataac cagcggggag attcaggcag ttcgaatcga
   109021 ccaatcaaaa tcaacaaaca ggggatcgcc gcgctaccca gcatccagcg ccagccgcct
   109081 tccacatcat aaagccagta gccgaccaga tcggcacagg tcgcgccgac ataccacatc
   109141 gcggcaataa agctgatgga aaacgcccgc tgacgggtac tggagaactc ggtgatcatt
   109201 gaggtggcga tgggataatc tgcaccgatg acaatgccga taagtacccg catcaccaac
   109261 agttcgacgg gggatgaaac aaacatcgtc gccaccgata tcacgccgat ggcgatgata
   109321 tcaatgagga acattttgcg ccgtccgact ttatcggaga tatagccaaa cagcgatgtg
   109381 ccaacgaaca gcccggcgag cgttcccgcg cccagcaagc caatccagtc agcgtccagt
   109441 ttcagcgccg gggtcagttg ctccagcgcc acgccaatca ttaccagtac ataaccatcc
   109501 agaaacggac caccgcttcc ccacagcaaa atgcggcggt gaatagagga gaatttgaga
   109561 tcgtcaaagt ttctggacgg ttgcatgggt tattcctgtt tttttgcaat gagagtcagt
   109621 acggcgttac gtatcaaacc aacatcagcc gtaacggaac tccacaccaa aggtgccgcg
   109681 cgggtattcc cactgttcca gcgccgaccc cagccccaga atgcgacagg tgccgcactc
   109741 cagacatccg gcgtaatcga agcgcacact gccgtcatcc tgcttcttgt acagacctgc
   109801 ggggcacgct ttcaccagca gctccagcgc ctgtttatca gcatcggcct tcacaacaat
   109861 gtgcggatgc tcttcatcga cattgaattt attgacgccc agtttgacgt ccacattgac
   109921 gggagaagtc ataaaacggt cactcctttc atgccatcct tgattagatt gatgaagccc
   109981 actttcttac cgtggcggag gattttcttg cgcatcagtt ctggcgcact gccgtcaatg
   110041 gtgaacaggt cacgcgccac gcccaccgcc agttccgggt agccgctaaa catgcgtggg
   110101 ttatcaagga acgccggtag tttctgatac atacgcatat cgcgcagcgg accactctca
   110161 agatgctgac gatattccgc cagtttttgc ttactgaaat cgtcgctttt catcgctgaa
   110221 agcacggttt ttgctgcggc ttccccggcg gcaatcgcca gatccatacc gcgaatggta
   110281 aaaccgaggt tcatacacat tccggcggca tcaccggcaa tcaatacgcc gtcaccaacc
   110341 aactccggca gcatgttgat gcctgcttcc ggcactacgt gagcggaata ttccaccagc
   110401 ttgccgcccg cgatcagcgg tgcaactgcc ggatgctgtt tgaaatcttc cagcatttgc
   110461 ggcaccgatt ttttcgcgtc atgcagatga tgcagaccac aaaccagccc cagcgacagg
   110521 gtgttttcat tggtataaag aaaaccgccg cccatcaggc catcggtggg tgatcccgca
   110581 aacaggcaag ccgccccctg attaccctgc aactgaaaac ggtcttcaat aaccgacttc
   110641 ggtaactcga tcagttcctt cacgccaacc gccacatccg tcggtttgac gcgttttgcc
   110701 atccccaatt tttcggcaag gatggagttc accccatcag caaggatcac cgttttcgct
   110761 tcaatcacat cgccatcggc ttctacaccg acgactttgc catcgcgctg tacgaggtta
   110821 tcgacacgga tcccggtaat taactgcgcg cccgcttctt cggcctgctc catcaaccag
   110881 gcatcaaatt tactgcgcaa aacggagtaa gaacgctggg atggcgaggt ttcgtcaccg
   110941 ttgcagtagt ccatagtcat cgctgacttt tccgtcataa acgcgagttt ttcatgggtg
   111001 atcaggcgtt ctacgggggc ggattcggcg aaacctggaa taatgtgttc cagactgtgg
   111061 gcatagagac gcccgccagt aacgttcttg gcacctgcgg aattgccacg ctcgataact
   111121 aacacttgcg ccccttcgcg ggcgagcacc agtgcggcaa ccgaaccggc aagccctgca
   111181 ccgacgatga tggcgtcaaa gatatcttcg gacataagag ctccctgtca gcggcagagc
   111241 cgtaatgcag ccctgccaga gtggatcaac gcgctaaagc tgcggtcagt gccggaagga
   111301 tcttcacggc atcgccaaca atgccgtaat ccgcgtactg gaagatcggc gcatttttat
   111361 ctttattgat ggcgaaaatg gtttgcgatg cattagcacc aaccatgtgc tggatctgcc
   111421 cggagatccc caccgccaga tacaattcag gtttcagcat caggttggag ataccgacat
   111481 agcgttcgtg ctccatccat ttttcgtttt ccgccaccgg acgagaacag gccaactccg
   111541 cacctatcgc cttgcaaagc tgttctgcca gcgcaatgtt ctctttgctg ccaatgccgc
   111601 gaccgacgct gactaccaga cgggctttgt cgagatcgac gctgttgctc tggcgcgcct
   111661 gggttgccgt gcgggtaatc gccacagccg gagcctgcca ctccacggtg tgcgtttcac
   111721 cagtgcgtga cgcgtctggc tgagccgcat cgaacgtgcc gctgctgatg gtcagtaccg
   111781 catacggcgt ggcaatgcgt tcttcgccaa tcgccagacc gccgtaaacc atgtgtttca
   111841 ctgtcgcttt accgtcctgt acgctgacgg tgctggcatc gttagacacc gccgctttaa
   111901 ggcgataacc cagttttgcc gccagtaatt tgccgcgacg ggtgtttggc aacagcacca
   111961 gaccgtctgc gccgtgctgg cgaatagtgt cagccatgac accggcgtaa tcttcgatca
   112021 tccgatcgtc cggtttgccg tttaatttcc agacatgatt agcgccgagc tggattgcct
   112081 gtgcgccgtc ggcatcattg aggacaaagg cattgatttg attagctaaa gcctgcgcac
   112141 cgttcatcag ttccggcaga cgagaagggg tatcgctgaa tacccagact tgagaaaacg
   112201 tgttcatagc atcccctgta attaaatgac tttgcgaaga ttttcagcaa atgcggcgat
   112261 ctgttcttcg ccgtcgcctt caatcacgat gcgctgacgc tcgcgctgtt tcggcgcggc
   112321 aacctgttgt tctgaccagg ctgcctctgc gttaaaaccg atatccgccg ccgaccatac
   112381 ctggactggc tttttcgccg cgccgagaat ggctttcatc gaaggaattt gtggggagtt
   112441 gatatcagtg gaaacagcaa caaccgcagg cagcggaatg cttaacgttt ctgtttcatc
   112501 ttccagttcg cgctcaacgg tgagggtatc tgccgtcagg gagataattt tgctgacgcc
   112561 gttaactgcc ggaatattga ggatttcgcc taccagcaaa ccaacctgct gggcataaag
   112621 gtcggaagaa ccatcgccac agaggatcaa atcaaagcct gctttctggg cggctgcagc
   112681 cagtgcgctc gccgtttgtt gcggcagtgc ctgctcgaac tggtcatcaa ttaccacaat
   112741 cagttcatcc gggccgcgcg ataacacatc tttacgccct ttggcgttgg tcagggcttt
   112801 accgcccaca cttaaggctg tcacctgcgc ctctgctgcc tgttgcttta gctggcaagc
   112861 cgcttcaata gcgttgagat cgtattggct tattttggca tcggctttgc tgaagtctaa
   112921 tgaaccatca gcattattga ccgcaatatc ctgttcatca ggcacgcact tatagcaagt
   112981 aataatcttc attgcatctc cagaaatcat gaaggaaacg tcgttcatat cggtggtgtt
   113041 aattacagat atcacgccct ttaatttgga aataaaactt taatcaccaa tattgaaaat
   113101 gtcacacgca gataaatcca actttcaata ttgttaagtt cctcaccaat attgaaaaca
   113161 cggcgtagca aaaagaaatt ttcaatattg ttttatggat caccaatatt gaaagtcaga
   113221 tgttattttt ttactcacaa tagagcataa caaactgatt attaatcaaa attaacgaaa
   113281 aaacgccctc tattcacccg aaacaaaaat gtgataccaa tcacagaata cagcttattg
   113341 aatacccatt atgagttagc cattaacgcg tccacgaggt taataataat tatattaaat
   113401 gttaacaaaa ataaaacaaa cgggaacgca aaataaatat tcgttttcac agtggaatta
   113461 actcatgaag aatgaaaaga gaaaaacggg aatagaaccg aaggttttct ttccgccgtt
   113521 aataatcgtc ggcatacttt gttggcttac agtcagagat ctggatgcag cgaatgtcgt
   113581 tattaatgct gtattcagtt acgtcaccaa tgtatgggga tgggcatttg aatggtatat
   113641 ggtggtgatg cttttcggtt ggttctggct ggtgtttggc ccgtatgcca aaaagcgttt
   113701 aggtaacgaa ccgccagaat ttagcaccgc cagttggatc tttatgatgt tcgcctcctg
   113761 tacgtctgct gccgtactgt tctggggatc gattgagatc tactactaca tctccacccc
   113821 gccgtttggc ttagaaccga actcgacagg ggcgaaagag ttggggctgg cttacagctt
   113881 gttccactgg ggacctctgc cgtgggccac ttacagcttc ctttcagtcg ccttcgctta
   113941 cttcttcttt gtccgcaaaa tggaagtgat tcgccccagc tcgacactgg tgccgctggt
   114001 aggtgaaaaa cacgccaaag ggttgttcgg cactatcgtc gacaacttct atctcgtcgc
   114061 cttgatcttc gcgatgggta ccagtctggg ccttgccacg ccgctggtga ccgagtgtat
   114121 gcaatggttg tttggcattc cgcataccct gcaactggac gctatcatca ttacctgctg
   114181 gattatcctc aacgccattt gcgtcgcttg cggtctgcaa aaaggggtac gtatcgccag
   114241 tgacgtgcgt agttacctga gcttcctgat gctgggttgg gtgttcattg tcagcggtgc
   114301 cagcttcatc atgaactact tcaccgattc ggtggggatg ttgctgatgt atctgccgcg
   114361 catgttgttc tataccgatc ccatcgctaa aggcggcttc ccgcagggct ggaccgtgtt
   114421 ctactgggca tggtgggtga tttatgctat ccagatgagt atcttcctcg cccgcatctc
   114481 ccgtggtcgt actgtgcgtg aactgtgctt cggcatggtg ctggggctga cagcgtcaac
   114541 ctggatcctg tggactgtac tcggtagtaa cactctgctg ttgatagata aaaacatcat
   114601 caacattcca aatctgatcg aacagtacgg tgtggcgcgc gccatcattg aaacctaggc
   114661 cgctctgcca ctcagcaccg ccaccatgtg gggcttcttc atcctctgct ttattgccac
   114721 cgttacgctg gttaacgcct gctcttatac cctggcgatg tccacttgcc gcgaagtacg
   114781 cgatggtgaa gaaccaccgc tgctggtgcg tatcggctgg tcaattctgg ttggcattat
   114841 cggtattgtt ctgctggcgc tcggcggcct gaaaccgatt caaaccgcca ttatcgccgg
   114901 aggatgcccg ctgttcttcg tcaacattat ggtgacgctc tcctttatta aagacgcgaa
   114961 acagaactgg aaagattaat taacccccaa aatatcaaga ggttgaaaga tggattttaa
   115021 tttaaatgat gagcaggaac tgtttgtcgc cggtatccgc gaactgatgg ccagcgaaaa
   115081 ctgggaggcc tattttgccg agtgcgaccg tgacagcgtc tacccggaac gttttgtcaa
   115141 agcactggcg gatatgggta tcgacagtct gctgatccct gaagagcacg gtggtctgga
   115201 cgcggggttt gttactctcg ccgccgtgtg gatggagctg ggacgtctgg gggcaccaac
   115261 ctatgtactg taccagttgc cgggcgggtt caataccttc ctgcgcgaag gcacacaaga
   115321 gcagatcgac aagattatgg ctttccgcgg caccggtaag cagatgtgga actcagcgat
   115381 taccgaaccg ggcgcgggct ccgacgtggg tagcctgaaa acgacttata cccgtagaaa
   115441 tggtaagatt tatcttaatg gtagtaagtg ttttattacc agtagcgcct acaccccgta
   115501 catcgtggtg atggcgcgcg acggggcttc tccggacaaa cctgtctaca ccgaatggtt
   115561 tgttgatatg agcaaaccgg gcatcaaagt gaccaaactt gagaagctcg gtctgcgtat
   115621 ggatagctgc tgtgaaatca cctttgacga tgtggaactg gacgagaaag acatgttcgg
   115681 tcgggaaggt aacggcttta accgcgtcaa agaagagttc gaccatgaac gtttcctggt
   115741 agccctgacc aactacggta cggcgatgtg cgcctttgaa gatgcggcgc gctacgccaa
   115801 ccagcgcgtg cagtttggcg aggctattgg tcgtttccag ttgattcagg aaaaattcgc
   115861 ccacatggcg atcaaattaa actccatgaa aaacatgctg tatgaagcag cgtggaaagc
   115921 agacaacggc accatcacct ctggcgatgc agcgatgtgc aaatacttct gcgccaatgc
   115981 ggcatttgaa gtggtggata gcgcaatgca ggtgctgggc ggtgtcggga ttgcgggcaa
   116041 ccaccgcatc agccgcttct ggcgtgacct gcgtgtagac cgcgtttccg gaggatctga
   116101 cgaaatgcag atcctgacgc tgggtcgtgc ggtgctgaag caataccgct aagtccgtgc
   116161 gctgcctgat gcgacgctga cgcgtcttat caggcctacg ggagcacgaa tgtaggccgg
   116221 ataaggcgtt tacgccgcat ccggcagtca tgcgctacat aaatttttca ggagctaata
   116281 atggatcatc tacccatgcc gaaattcggg ccgttggccg gattgcgcgt tgtcttctcc
   116341 ggtatcgaaa tcgccgggcc gttcgccggg caaatgttcg cagaatgggg tgcggaagtt
   116401 atctggatcg agaacgtcgc ctgggccgac accattcgcg ttcaaccgaa ctacccgcag
   116461 ctctcccgcc gcaatttgca cgcgctgtcg ttaaatattt tcaaagatga aggccgcgaa
   116521 gcgtttctga aattaatgga aaccaccgat atcttcatcg aagccagtaa aggtccggcc
   116581 tttgcccgtc gtggcattac cgatgaagta ctgtggcagc acaacccgaa actggttatc
   116641 gctcacctgt ccggttttgg tcagtacggc accgaggagt acaccaatct tccggcctat
   116701 aacaccatcg cccaggcctt cagtggttac ctgattcaga acggtgatgt tgaccagcca
   116761 atgcctgcct tcccgtatac cgccgattac ttttctggcc tgaccgccac cacggcggcg
   116821 ctggcagcac tgcataaagc gcgtgaaacc ggtaaaggcg aaagtatcga catcgccatg
   116881 tatgaagtga tgctgcgtat gggccagtac ttcatgatgg attacttcaa cggcggcgaa
   116941 atgtgcccgc gcatgagcaa aggtaaagat ccctactacg ccggttgcgg tctgtataaa
   117001 tgtgccgacg gctacatcgt gatggaactg gtgggcatta cccaaattga agagtgcttt
   117061 aaagatattg gcctcgcaca tctgcttggc acgccagaaa tcccggaagg cactcagctt
   117121 atccaccgta tcgaatgccc ttacggccca ctggttgaag agaaactcga tgcctggctg
   117181 gcgacacata ccatcgcgga agtaaaagaa cgctttgctg aactgaatat cgcctgcgcc
   117241 aaagtgctga ccgtaccgga actggaaagc aatccacagt atgtggctcg cgaatcaatc
   117301 actcagtggc aaacgatgga tggtcgcacc tgcaaagggc cgaacatcat gccgaaattc
   117361 aaaaataacc ccggacaaat ctggcgcgga atgccctcac atggcatgga cacggctgcc
   117421 attttgaaaa atatcggcta cagcgaaaac gacattcagg agttggtcag caaaggtctg
   117481 gccaaagttg aggactaaac gcgtggcgtc agtcggcttg cctgactagc gcccgcccaa
   117541 taacaaacga acaaaaatgg atagaggtgc aatggatatc attggcggac aacatctacg
   117601 tcaaatgtgg gacgatcttg cggacgttta cggtcataaa acggcgctga tttgtgaatc
   117661 cagcggcgga gtcgttaacc ggtatagtta tcttgagtta aatcaggaga ttaaccgcac
   117721 ggcaaacctg ttttatacgc tggggattcg caaaggcgac aaggttgcac tacatctcga
   117781 caactgcccg gaatttatct tttgctggtt cgggctggca aaaattggcg cgattatggt
   117841 gccgattaac gcccgcctgt tacgcgagga aagcgcgtgg atcctgcaaa atagccaggc
   117901 gtgcctgctg gtgaccagtg cgcaattcta tcctatgtat caacagattc agcaggaaga
   117961 tgccactcaa ttgcggcaca tttgcctgac agatgtggca cttcccgctg atgatggcgt
   118021 gagttcgttt actcaactga aaaatcaaca acctgccacc ttgtgctatg caccgccgct
   118081 atcgactgac gatacggcgg aaattctctt cacctccggc accacctccc gaccgaaagg
   118141 tgtggtgatt acccattaca acctgcgctt cgctggatat tactccgcct ggcagtgtgc
   118201 actgcgtgac gatgacgtct acctgacggt aatgcctgcg tttcatatcg attgccagtg
   118261 tactgcggcg atggcggcgt tttctgccgg ggccaccttt gtgctggtcg agaaatacag
   118321 cgcccgcgcc ttctggggac aggtacagaa gtaccgcgcc accgttaccg aatgtattcc
   118381 gatgatgatc cgtaccttga tggtgcagcc gccttcagcg aacgatcggc aacaccgcct
   118441 gcgggaagtg atgttttatc tcaacttgtc ggagcaggaa aaagatgcgt tttgtgaacg
   118501 cttcggcgtt cgcttgctga cgtcttatgg gatgacggaa accattgtgg gcattatcgg
   118561 cgatcgccct ggcgataaac gacgctggcc gtcgattggt cgagcggggt tttgctacga
   118621 agcggagatc cgcgacgatc acaattgccc gctcccggca ggtgagatcg gtgaaatctg
   118681 tattaaaggc gtaccaggga aaaccatctt caaagagtat tttctcaacc cgaaagccac
   118741 tgcgaaagtg ctggaagctg atggctggct gcataccggc gataccggat accgcgacga
   118801 agaaggcttt ttttatttcg tcgatcgccg ctgcaatatg atcaaacgcg gtggcgagaa
   118861 tgtctcctgc gtggagctgg aaaatatcat cgccacccat ccgaaaattc aggacatcgt
   118921 ggttgtgggt attaaagatt cgattcgcga tgaagccatc aaagcatttg tggtgctgaa
   118981 tgaaggtgaa acattgagcg aagaggaatt ttttcgcttc tgcgaacaaa atatggcgaa
   119041 atttaaagtg ccctcttatc tggagatcag aaaagatctg ccacgtaatt gctcggggaa
   119101 aataatcaga aagaatctga aataaatgaa acggcaggga actaccctgc cagctaataa
   119161 tcacgctcta aaacaatatg cattttttgc aggaatgttg tcgtccctga aaaagcaaaa
   119221 atggagaaaa ggaatgagtg aatcattaca tctgacccgc aatggatcaa ttctggaaat
   119281 tacccttgat cgcccaaaag cgaatgctat tgatgcaaaa accagctttg aaatgggcga
   119341 agtatttcta aatttccgtg acgatccgca attacgtgtc gccattatta ccggtgccgg
   119401 agagaaattc ttttccgcgg gctgggattt aaaagcggca gcagaaggcg aagcaccgga
   119461 tgctgacttt ggcccgggtg gttttgcggg attaaccgaa attttcaatc tcgacaaacc
   119521 agttatcgca gctgtgaacg gctacgcctt tggcggcggt ttcgaactgg cgctggcggc
   119581 agattttatt gtttgtgccg ataacgccag cttcgccctg ccggaagcca aactgggcat
   119641 cgttcctgac agcggcggtg tgctgcgtct gccgaagatc ctgccgcctg ccatcgtcaa
   119701 tgaaatggtg atgaccggca gacgaatggg cgcagaagag gcgctgcgtt gggggatagt
   119761 caaccgcgtg gttagccagg cggaactgat ggataacgcc cgcgaactgg cgcagcagct
   119821 ggttaacagc gctccgctgg cgattgcggc gctgaaagag atctaccgca ccaccagcga
   119881 aatgccggta gaagaagcgt atcgctatat tcgcagcggc gtgttgaaac actatccgtc
   119941 ggttctgcat tcggaggatg ccattgaagg gccgctggcg tttgccgaga agcgcgatcc
   120001 ggtgtggaaa ggacgttaac aaccgtgagc tattacgcct ttgaggggtt aattccggtg
   120061 gttcacccga cggcgtttgt ccatcccagt gccgtcttga ttggcgatgt gattgtggga
   120121 gccggtgtct acatcggccc actcgcctca ctgcgtggtg actacgggcg gttgatcgtg
   120181 caagcgggag ccaatattca ggatggctgc attatgcatg gctactgcga cactgacacc
   120241 attgttgggg aaaacggcca tatcgggcac ggagcgatcc tgcatggttg tgtgattggt
   120301 cgcgatgcat tggtcgggat gaacagcgtg attatggatg gcgcggtcat tggcgaagag
   120361 agcattgttg ccgccatgag ctttgtcaaa gcgggcttta gcggcgagaa acgccagttg
   120421 ttgatgggta cgccagcccg cgctgtacgc agtgttagtg acgacgagtt acactggaaa
   120481 cggttgaata ccaaagagta tcaggatctt gtcgggcgct gtcatgcatc gttacatgaa
   120541 acgcagccgt tgaggcaaat ggaggaaaat cgcccccgtt tgcaggggac aacggatgtg
   120601 acgcctaaac ggtgatcttg ccggatgcgg cgtaaacgcc ttatccaact ctgctggaga
   120661 gcaaccgtag gccggataag atgcgccagc atcgcatccg gcgatagctg tcggatgcgg
   120721 cataaacgcc ttatccaacc cggctggaga gcaaccgtag gctggataag atgcgccagc
   120781 attgcatcca gcaaccaggc tgcgtggtat caacgaaatg acattaacga cgcatactct
   120841 ttgacaacat catctgccac ttctgctcgc ggttaagttc cgtgagcgga acggcaggaa
   120901 tacgcggcgt ctttcttaca ccgactaatt tttcccgact gttattgcgc aggcgcgcgt
   120961 aaatttgctc atcgatatcg accactttaa ccagtcgctg acactggcat ccccgccctt
   121021 ccagcttatt agggatcatc ttaacttcgc agcttatttc tgtgacttcc gacagaatat
   121081 aagtcagggt attaaccgcc ttgctgtgtt caatatcgaa atgaatagag atctctcttg
   121141 ctatcaccca ccgattttca gccatcatcc attcggcgat taacaagtag agtggttttt
   121201 caacatatcc ttcacacata cgaactccag tttcacaaat taaaagtctg gctatgactc
   121261 gttttgaagc aattaaacaa ggccatatta aaattgtgga tatatcgata gtatgcaact
   121321 tcaccgtcga taagtgtgag ctaaatcctg cttatgtcat caaaaatatc gattccccaa
   121381 aagatttact taatgggcaa aaaaaaacgg tcctcatcag agaaccgtat tagttattcg
   121441 atcaaattag cggatgaaaa atatctgcca tgacacgcta ttatttgatc tgtgcgtgca
   121501 tttcctgcac cgaaattact ttttcagtcg catcggcatt cagcgccatc gcggtggcaa
   121561 agccgccgtt cagggtggtg tcgtaatgca ctttatattg cagcgcactg cgacgaatca
   121621 cgcgggagtc ttcaatcgca cgacggcctg aggtggtgtt gatgatgtag gtatattcgc
   121681 cattcttgat acggtcctga atgtgcggac ggccttcatg caccttgttt accagacgcg
   121741 ggttgatacc tgcttcgccc agcacaatcg ccgtgccgtg ggtcgcatcc agctcgaagc
   121801 cctgtttcag cagttttgcc gccaggtcca ccacgcgttc tttatcgcct tcgcgcacgg
   121861 aaagcagcgc acgaccgtgt ttcttcatgg tggagttgct gcccagctgc gctttggcaa
   121921 acgcttcagc gaaggtgcgg cccacgccca tgacttcccc ggtagagcgc atttctggcc
   121981 ctaacagcgg gtcaacgccc gggaatttat tgaacggcag caccacttct ttcaccgagt
   122041 agtacggcgg gataacttct ttggttacgc cctgctcagc cagcgatttg ccagccatca
   122101 cacgcgccgc cacttttgcc agcggtacgc cggtggcttt ggagacgaac ggaacggtac
   122161 gcgccgcacg cgggttaact tcaatcaggt agacttcgtt gtttttcacc gcaaactgca
   122221 cgttcatcag accgcgcacc tgcaattcga aggccagttt ctgcacctgc tggcgcatca
   122281 catcctgaat ttcctgactt aaggtgtagg ctggcagaga acatgcggag tcaccggagt
   122341 gcacgcccgc ctgctcgatg tgctccatga tgccgccaat aagcaccatt tcgccgtcgc
   122401 agatggcatc cacgtcaact tctaccgcgt catcaaggaa gtgatccagc aacactggcg
   122461 catcgttaga cacgctgacc gccgtctgga agtagcgacg caggtcagct tcgtcataga
   122521 cgatttccat cgcccgaccg ccgagaacgt aagacggacg taccaccagc gggtagccaa
   122581 tctctttcgc cttctctacc gccatttcaa tagcggtaac ggtggcgttc gccggttgtt
   122641 tcagtttcag gcggtcaacc gcgtgctgga agcgttcacg gtcttccgca cggtcgatag
   122701 catccgggct ggtgccgata accggtacgc cagcggcttc cagtgcacgc gccagtttca
   122761 gcggggtctg accgccgtac tgaacgataa cgcctttcgg cttctcaata cgcacgattt
   122821 ccagcacatc ttccagggtt accggctcga agtagaggcg gtcggaagtg tcgtaatcgg
   122881 tggagacggt ttccgggtta cagttaacca taatggtttc gtaaccgtct tcgcgcagcg
   122941 ccagcgaggc gtgtacgcag cagtagtcga attcgatacc ctgaccgata cggttcgggc
   123001 cgccgccaag caccatgatt ttttcacggt cggtagacgg attcgcttcg cactcttctt
   123061 cataagtgga gtacatgtaa gcggtgtcgg tggcgaactc tgccgcacag gtatccacgc
   123121 gcttataaac cgggtgcagg tcatactggt cacgcagctt acggatttcc gcttcgcgta
   123181 cgcccgccag ttttgccaag cgcgcatcgg caaagccttt gcgtttcagc tggcgcagga
   123241 agtcagcgtt caggccagtg atgcccactt ccgccacttt ctcttccaga cgcaccagct
   123301 cttcaatctg taccaggaac cagcggtcaa tgttggtcag gttgaagacg ccgtccacag
   123361 acaggcccgc acggaacgca tcggcgatgt accagatacg atctgcgcct gcgtctttca
   123421 gttcgcgacg gattttggtt aacgcttccg ggtcatccag gctcactttc gggtcgaatc
   123481 cagtcgcacc gacttccagg ccgcgcagcg ctttttgcag ggattcctgc tgcgtgcgac
   123541 caatcgccat cacttcgcca accgatttca tctgagtggt cagacggtcg ttagcaccgg
   123601 cgaatttttc gaagttgaag cgaggaattt tagtaaccac atagtcgatg gacggctcga
   123661 aggaggccgg agtacgtccg ccagtgatgt cgttcatcag ttcgtcgagg gtgtaaccca
   123721 ccgccagttt cgccgccact ttagcaatcg gaaaaccggt cgctttcgac gccagcgccg
   123781 aagaacggga cacgcgtggg ttcatttcga taacaatcag acgaccgttt ttcgggttca
   123841 ccgcaaactg aacgttggaa ccaccggttt caacgccgat ttcacgcagc accgccatcg
   123901 aggcgttacg catgatttga tattctttgt cggtcagcgt ttgggctggc gcgacagtga
   123961 tggagtcacc ggtgtggatg cccatcgcat cgaagttttc gatagagcag acgatgatgc
   124021 agttgtcgtt tttatcacgc accacttcca tctcgtactc tttccagccg atcagcgact
   124081 catcaatcag caactctttg gtcggagaga gatccagacc gcgggcgcaa atttcttcaa
   124141 actcttcacg gttataagcg ataccgccgc cgctaccgcc catggtaaag gatgggcgaa
   124201 taatgcacgg gaagcccacg tcagcggcaa ccgccagcgc ttcttccatc gtgtgtgcga
   124261 taccggaacg cgcggtttcc agaccaattt tcttcatcgc tacgtcgaaa cgacggcggt
   124321 cttctgcttt atcaatcgca tcggcagtgg caccaatcat ggtgacaccg aactcttcca
   124381 acacgccctg acgttccagc tccagcgcgc agttcagcgc cgtctgaccg cccatcgttg
   124441 gcagcaccgc gtccgggcgc tctttttcaa taatcttgcg tacaacttcc cagtgaatcg
   124501 gctcgatgta ggttgcatca gccatttccg ggtcggtcat gatggtcgcc gggttggagt
   124561 tcaccagaat gacgcggtaa ccctcttcac gcagggcttt acacgcttgc gcgccagagt
   124621 agtcaaactc acacgcctga ccgataacaa tcgggcccgc acccagaatc aggatacttt
   124681 ttatatctgt acgttttggc atggctcttt tactcctgat tacttagcgg ttttacggta
   124741 ctgctcaatt aactcgataa agtggtcgaa caacggcgcg gcgtcgtgtg gaccagggct
   124801 ggcttcaggg tgcccctgga agctgaacgc cggtttatcg gtgcgatgaa tgccctgtaa
   124861 cgtaccgtcg aacagggatt tatgcgtgac acgcaggttt gcaggtaatg ttgcttcgtc
   124921 caccgcaaaa ccgtggttct gggcggtgat cattaccacg tttttctcca catctttaac
   124981 cggatggttg ccgccgtggt gaccaaattt cattttgaca gtcttcgcac cgctcgccag
   125041 cgccagcagc tgatgaccga gacagatgcc gaataccgga atatcggttt cgaggaattt
   125101 ctggatggcg gtaatggcgt aatcgcacgg ggccgggtcg ccaggaccgt tggagaggaa
   125161 gatgccgtct ggattcattt tcagcacatc ttccgcagaa gtttgcgccg gaacgatggt
   125221 caggcgacag cctctatcca ccagcatccg caggatgttg cgcttggcac caaaatcata
   125281 agccacgacg tggaacggca gctcgtcttc ttttttcgct tctggcaggc caccggtcaa
   125341 cgtccagctc ccttgtgtcc agctataggc ttctgcggtg gtcacttctt ttgccagatc
   125401 catgccattc agacctggga acgcgcgggc tttttctaac gccagcgccg catccgggtt
   125461 atcgcccgcg ataatgcagc cattctgtgc gcctttctcg cgcagtaaac gcgtcagctt
   125521 acgggtatcg atatcggcaa tcgccacgat gttatggcgt ttcaggtaag aagagaggtc
   125581 ttcggtatta cggaagttgc tggcaatcag cggcaggtcg cgaatcacca gaccttgtgc
   125641 atgtacctga gaagattctt catcggcgtc attagtgccg acattgccaa tatggggata
   125701 agtaagagta acgatttgac gagaatagga aggatcagtg aggatttctt gataaccggt
   125761 cattgaagta ttgaaaacga cttccccaac cgccgaacct gttgccccta tggcccgacc
   125821 gtgaaactgg gttccgtctt ccagaaccaa tagcgctgac ttaatcaaaa caccctccag
   125881 agaatattca ctcactttat ttgcatatta attcacaatg atgacgtaaa tcaatgcaaa
   125941 tctgcttacc gacgaatttc tggcaaacgg cggcattctg gagatatggg cgctaaaagt
   126001 caacttaatg gcgggatttt ttatattatt ttgtggctca tgcatgtttt acaggcatta
   126061 agcgacaaaa agatcatcta acctgttctg gaaaacgctt gcgcagcaag aagattgcat
   126121 gaagtaaaaa cgagtagaaa aaagtggacc aaatggtcaa aatttacatt aaaacaacaa
   126181 aaatagaaga caaaacatgt gatttttagc gtttgcataa aatttaaaaa cataacacaa
   126241 aaaataaaag ggctaaaaat taaagccctt tatattcaat aaattaaatg cgttactttt
   126301 gcatcactat aaagattttg tggttacaaa ctatggagtt caagcacatc tcgcatatca
   126361 aaaagaccac tttccttgcc actcaaccac aaagccgatc ttaccgcacc gttagcaaat
   126421 gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
   126481 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtaccaggc
   126541 acacgttcac cggtatggcc ttcacgactg tagaccgcgc aatctttcag atctttatca
   126601 agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
   126661 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct
   126721 ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc
   126781 gcaatatcgg cggcggcgtc acgaattgct tgtttaccgg cttcgtcaaa ccccgtagtg
   126841 ccgatcacca tccctttgcc atgctggcga caaaaagcga gatggttcag cgtaccttcc
   126901 ggacgggtaa aatcgataaa cacatcaaaa tcatctttta ccgcatcgag gctgctttgc
   126961 acggtaacgc ctgttttccc ggctccggcc agctcaccgg cgtcgctgcc cagtaaagaa
   127021 gatccttcac gctccagcgc agcgcccaac tgcacgccct ctaatgccag cgccgcctga
   127081 atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca
   127141 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
   127201 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
   127261 agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag gttacggcac
   127321 cagagccagc acctcagcta cccactgctg gaagcctttt acattcagat ccaatgccac
   127381 ctgtacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt
   127441 aaattcgcct tgagtttcta ccgcgacaaa acacggcttg agggtgaata agtccgggcg
   127501 caccagccag gcgatggcgc agagatcgtg cattcgcaga ccgctttgca tactgccgct
   127561 acggtagtgg ctaaacaggg cgtggagcat ttttccggtg cgattcaact ccggcaaagt
   127621 agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc acatgacaat
   127681 ttcgattccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc
   127741 ggcgtttggt gtgcagttgc cgcgaccggc agaaccaccc atgatcacca gacggcgaat
   127801 atgcggtttg cattccgggc attgtgaaag taacagcgca atattggtta acgggccgat
   127861 ggctaccagg gtaacaggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc
   127921 cggtacctcg agcggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc
   127981 gccgtgcaca gatgccgcat cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc
   128041 gagcggaatc tccgcattcc agaaatgcag cagttgcaag gcattacggg tagttttctc
   128101 aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gtgcaaaaat
   128161 cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa
   128221 acgcatgttt tctccataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg
   128281 ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa
   128341 acaatgtttt cttcacgtcc ttccagcgga atggcttcac caccgcccag ctgttgcaaa
   128401 cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg
   128461 catttaacct ctttcaccca ctcttcctgg atatctttcg catcatcaat caaaaacgcg
   128521 tttttgccca tacgctgggc cagctccgcc agacggttgg agttagagga gtttttcgaa
   128581 ccgaccacca gcacaacttc cgcctgttcg gccagggcac gtaccgcttc ctgtcggtta
   128641 gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc
   128701 agcgcgtcga tcacatcaga cgtgtcatcc accgacagcg tggtctgggt cataaaggag
   128761 agtttctctt cgtttttgac cgtcagtttc cacacatcgt ctggtgattc aaccagatac
   128821 attccccctt ccgggttgct gtactgaccc atcgtgcctt ccacttccgg gtgcccggcg
   128881 tgaccgatga gaatagattc ttcgccacgg cgactagcgc gggcgacttc catatgcact
   128941 ttggtcacca gcgggcaggt ggcgtcgaat accgtcaaat cacggctttt cgcttcgtta
   129001 cgtaccgcct gagaaacacc gtgtgcggag aaaatcagga ttgcgccgtc cggcacttcg
   129061 ctaatctgct caataaagat agccccacgc tcgcgcaggc tatcgaccac gtagcggtta
   129121 tgcaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca
   129181 atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc
   129241 atgttacgcc tccagtgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg
   129301 cccggccagc ggatggttga aatcaacggt aatggagtcg tcgttaattt cgcggatcac
   129361 gccaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc
   129421 gcctgcatcc ataaactcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc
   129481 aaacgccgca tctggctcca gcgagaaggt ggttttatcg cccactttca gccccagcag
   129541 gtgttgctcc agcccttcag aaagagaagc atcgcccagg cggaacagcg ccggtttacc
   129601 gttgttgcgg gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag
   129661 gacggcgcta ttgctctgta cagattcaga catgcaggtt tgctcttata tttgaggatt
   129721 tttgccggat gcggcgtaaa cgccttatcc ggcctacgat ttcgcagcaa tctgtaggcc
   129781 ggataagatg cgtcagcatc gcatccggca gggtttatta ttgttttttc gctttagaag
   129841 gtaaaaaacc ttccagcaca atcattgccg caccgacaca gatggcagta tcggcaaggt
   129901 tgaaggtggc gaagtgccag ccgccgacgt agaagtcgat catatcgaca acgaagccgt
   129961 gccacaggcg gtcgaacagg ttgcccagcg cgccgccaat aatcagcgcg taagcgatat
   130021 tgtttagctt ctgcgtggcc ttcgagcgat acatcatcac tgccaggatc acgctaatac
   130081 caatcgcaat accggcaaag aaccaacgct gccagccgcc gctatcggca aggaaactaa
   130141 acgccgcgcc atagttacgc gcataatgca gattaagcga cgggaacagc gggaccgtat
   130201 cccccagagc aaagttctgg aggatcaggt atttgctgcc cagatcgata atcagcacga
   130261 ctaccaccag ccacagccag cgtagccctg ttgaacagat cgattgactc atcaggcaaa
   130321 cttacgtttt tcaccgtcac cggcgacgtt gctgacacag cggccgcaga tttctgcgtg
   130381 ttccgccacc ttgccgacat cctgggtgta gtgccagcag cgtgggcact tctcaccttc
   130441 ggctttactc aacgcgactt tcagcccttt gagtacttcg ctctgctgag catcagcagg
   130501 tgcgtcgtta tagtctgcaa cggtagcgcc ggaggtcaac aagacaaatc gtaattcatc
   130561 gcccagcgcg gtcagtttcg ccgccagttc cggttctgca tacaaggtta ctgccgcttc
   130621 cagcgagcca cccactttct tgtcggcacg cgcttgctca atgactttgt tcacttcgcc
   130681 acgcactttc aacagctcgt cccagaacgc atcgttcatt gcttcactgt ctgccagacc
   130741 aaacaggcct tcgtaccact caccggtgaa gacgtatttt tcacgttcgc ccggcaggta
   130801 gccccacact tcatcagcgg tgaaggagag gattggtgcc atccagcgaa ccagcgcttc
   130861 tgcgatgtga tacagcgcag tctggcagct acgacgcgcc acgctgtccg ctttggcggt
   130921 gtactgacgg tctttgatga tgtcgaggta gaaggaaccc atctcaacgg agcagaagcg
   130981 catcagacgc tgtaccactt cgtggaaatc gtatgcttcg tacgccttga ggatgtcttc
   131041 ctgtgccgct ttcgcacaac ctacggccca gcgatccagt accaccatct cttccggttt
   131101 caccatatct tttgctggat caaaaccgtt caggtttgcc agcaggaagc gcgcggtgtt
   131161 acggatacga cgatagctgt cggcagcacg tttcaggatc tcgtcagaaa cggccatttc
   131221 accggtgtag tcggttgatg ccacccacag acgcagaata tccgcgccca gtttgttcat
   131281 cacatcctgc ggcgaaacgg tgttgccgat ggatttagac atcttgcggc cctgaccatc
   131341 cacggtaaag ccgtgggtca gtacctgacg atacggcgct ttgcctttca tcgcggtgga
   131401 gatcattagg gaagacatga accaaccacg gtgctggtca gaaccttcca gatacatgtc
   131461 cgctgcgtga ccggcaaatt ccggacgcac gtcaacaaca gaagagtggg tagatccgga
   131521 gtcaaaccat acgtcgagag tatccggcac tttcacatac tggtcagctt cgtcgccgag
   131581 gatctctttc gcatcgagat cccaccacgc ctggatgccg ttaacttcaa cgcgttttgc
   131641 cacttcttcc atcagttcga gggtacgcgg atgcagctct tccgtgtctt tgtgcacgaa
   131701 cagtgacatc ggtacgcccc aggtgcgctg acgggagata caccagtcag gacggttagc
   131761 aaccatcgac tcgatacgcg cctggcccca gtccgggatc cactgcacgc ctttgatctc
   131821 tttcagtgac tgcgcacgca gacctttctg atccatgctg acgaaccact gcggcgtcgc
   131881 gcggaagatg atcggcgttt tgtgacgcca gcagcacgga tagctgtgct gcattttctc
   131941 aacgtgcagc agcgcgcctt tttcctgcag cagcgcaacg acgatgtcgt tcgctttgaa
   132001 gacgttcacg ccatccagcg tcggataagt gcccggcaga taagtgccgt ccgggccaac
   132061 cgggttagcg gtttccaggc cgtatttctg accgatcaca tagtcgtccg ggccgtgacc
   132121 tggcgcggtg tgaacggcac cagtaccggc atccagggta acgtgatcgc cgaggattgc
   132181 cggaacgtcg aagcccataa acggatgggt aaagcgcagc agctcaagct ccgcaccttt
   132241 taccgtgccg agaatggtgt aatcggtcac gccgatacgc tgcattacgc tttcaaccag
   132301 atctttcgcc agaatcacgg cctgaccgtc gatctgcacc agcgcatagt caaaatcagg
   132361 tgcaatggag attgcgcggt tggcaggcag agtccacggc gtagtggtcc agatcaccag
   132421 cgagattggg ccgttaacgt tgcttacggc aaattttgct ttcagtgcat cctgatcgac
   132481 cgcctggaaa gcgacgtcga tggacggaga agttttgtcg taatactcaa cttccgcttc
   132541 cgccagcgca gaacggcagt caacgcacca gtgaactggc ttcgcgcctt tgtgcaggtg
   132601 accgttgccg atgattttgc ccagcgcgcg gatgatattg gcttcagttt tgaagtccat
   132661 ggtcaggtac gggtgcgacc agtcgcccag cacgcccaga cggataaagt ctttgcgttg
   132721 accgtcaacc tgggtcgccg cgtattcgcg gcacttggcg cggaactcgg cggcggtgaa
   132781 tttctcaccc ggcttaccgt attcttgctc tactttcagc tcgatcggca gaccgtggca
   132841 gtcccagcca ggcacatacg gcgagtcata accggaaagc cctttggact tcacgataat
   132901 gtctttcaga atcttgttaa ccgagtgacc aatatgaatg ctgccattcg cataaggagg
   132961 gccatcatgc agaatgaagg tttttttgcc ttttttagcc gcacggatga tgccgtacag
   133021 atcatcatca gtccaacgcg ccagcattcc cggttcgcgc ttggcgagat cgccacgcat
   133081 cgggaaccct gtttccggca aattcagggt tgatttatag tcactcatca gattctcggt
   133141 tccgtgtttc ggtttgatta cataacaggc ttaagccggt tttgttagcc caaaaaattc
   133201 gcgggcggtt aattcatcac gcgcaatctg cgctttcagt tcgtccagcg acgcaaatcg
   133261 ctgctcattg cgtatttttt tacgcagcac tacttgtata tggcgaccgt aaaggtccat
   133321 tgcaacatct aacaaatgca cttccagctg ctggcgaata ccggcaaccg ttgggcgtgt
   133381 tccaatgttt gccacgccgg gtaacggctt ttcaccgagg cccagcactt ctaccgcata
   133441 aacccctttc accggggaaa cctgacggcg tagcggtaca ttcgccgtcg ggaaacctat
   133501 agtgcgccct aattcatcac cgtggactac acgcccggag atggcaaacg ggtgccccag
   133561 taaactttct gccagagcca gattgtcatc cgccagcgcc tgacgcacag cggtgctgct
   133621 aatacgcacg ccaccttcgc aaaaagtttg cgtactagtg atatcgaagc cgtattccat
   133681 gccagctttc tgtaataaca agaaatcgcc ttcacgacca gcgccaaagc ggaaatcatc
   133741 ccctacggca agaaatttta cgcgcaaatg cttcaccaga agatcgctga tgaaattttg
   133801 cgcggttaac gccgcgaaac gcctgtcgaa acgcacgcac agcacgtaat caacgccaca
   133861 ctctgcaagg taacgcagtt tttcccgcag ccgggtcagt cttgccgggg ctttatcggt
   133921 agcaaacagt tccagtggtt gaggttcaaa aagcatcacc atcaccggta agttgcgctt
   133981 gcgcccttct tcctgcaagc cctgtaacag cgcgcgatga ccgcgatgca cgccgtcgaa
   134041 attaccaata gtcagcacac acccttcttg cggggcctgg ctgagattat gtatgccgcg
   134101 tatcagcttc atgtctggct caaaacagtg aaaatcgtcc gagtatacct tgtacagcgg
   134161 tcaaggttaa ccggcgattg agtaccgaaa tcagaaagaa gtggcttttc attgccatgg
   134221 cgcaaatcac gggaagaaag tgaccgcctg ctgcaatttt tatcgcggaa aagctgtatt
   134281 cacaccccgc aagctggtag aatcctgcgc catcactacg taacgagtgc cggcacatta
   134341 acggcgctta tttgcacaaa tccattgaca aaagaaggct aaaagggcat attcctcggc
   134401 ctttgaattg tccatataga acacatttgg gagttggacc ttggctaata tcaaatcagc
   134461 taagaagcgc gccattcagt ctgaaaaggc tcgtaagcac aacgcaagcc gtcgctctat
   134521 gatgcgtact ttcatcaaga aagtatacgc agctatcgaa gctggcgaca aagctgctgc
   134581 acagaaagca tttaacgaaa tgcaaccgat cgtggaccgt caggctgcta aaggtctgat
   134641 ccacaaaaac aaagctgcac gtcataaggc taacctgact gcacagatca acaaactggc
   134701 ttaatcgcca atttgctgaa gctttgtgaa aaagcccgcg caagcgggtt tttttatgcc
   134761 tgctgctttt atgatgcgtg agagaacaca ctaccggttg ccagatagtc aaaagatgct
   134821 ggcttttgca gacatgccgt tacactttct gaactcttcg tggcttaaga atcgtagaac
   134881 catcggctga tacattctga acaagaatat ctactcaatc tccagactcc cctacaaaga
   134941 atgtggatat tgtgatacac attgaggtat taaacttaag accactctat ttttgaatta
   135001 aattcctata cactttaacc attcataaat atctccttat tag
//
LOCUS       DAJOSY010000002        96406 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJOSY010000002 DAJOSY010000000
VERSION     DAJOSY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.002"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            4..522
                     /locus_tag="OHN27_000119"
     CDS             4..522
                     /locus_tag="OHN27_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCQ2284632.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            538..1077
                     /locus_tag="OHN27_000120"
     CDS             538..1077
                     /locus_tag="OHN27_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCQ2284633.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1170..2747)
                     /gene="guaA"
                     /locus_tag="OHN27_000121"
     CDS             complement(1170..2747)
                     /gene="guaA"
                     /locus_tag="OHN27_000121"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCQ2284634.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2816..4282)
                     /gene="guaB"
                     /locus_tag="OHN27_000122"
     CDS             complement(2816..4282)
                     /gene="guaB"
                     /locus_tag="OHN27_000122"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCQ2284635.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4444..5814
                     /gene="xseA"
                     /locus_tag="OHN27_000123"
     CDS             4444..5814
                     /gene="xseA"
                     /locus_tag="OHN27_000123"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCQ2284636.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLKKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5811..6026)
                     /locus_tag="OHN27_000124"
     CDS             complement(5811..6026)
                     /locus_tag="OHN27_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCQ2284637.1"
                     /translation="MELHCPQCQHVLDQDNGHAHCPSCGEVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6095..7567)
                     /gene="der"
                     /locus_tag="OHN27_000125"
     CDS             complement(6095..7567)
                     /gene="der"
                     /locus_tag="OHN27_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCQ2284638.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7685..8863)
                     /gene="bamB"
                     /locus_tag="OHN27_000126"
     CDS             complement(7685..8863)
                     /gene="bamB"
                     /locus_tag="OHN27_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCQ2284639.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8874..9494)
                     /gene="yfgM"
                     /locus_tag="OHN27_000127"
     CDS             complement(8874..9494)
                     /gene="yfgM"
                     /locus_tag="OHN27_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCQ2284640.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9512..10786)
                     /gene="hisS"
                     /locus_tag="OHN27_000128"
     CDS             complement(9512..10786)
                     /gene="hisS"
                     /locus_tag="OHN27_000128"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCQ2284641.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10897..12015)
                     /gene="ispG"
                     /locus_tag="OHN27_000129"
                     /gene_synonym="gcpE"
     CDS             complement(10897..12015)
                     /gene="ispG"
                     /locus_tag="OHN27_000129"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCQ2284642.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTSQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12042..13055)
                     /gene="rodZ"
                     /locus_tag="OHN27_000130"
     CDS             complement(12042..13055)
                     /gene="rodZ"
                     /locus_tag="OHN27_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCQ2284643.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPASTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13340..14494)
                     /gene="trmG/rlmN"
                     /locus_tag="OHN27_000131"
     CDS             complement(13340..14494)
                     /gene="trmG/rlmN"
                     /locus_tag="OHN27_000131"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCQ2284644.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14644..15075)
                     /gene="ndk"
                     /locus_tag="OHN27_000132"
     CDS             complement(14644..15075)
                     /gene="ndk"
                     /locus_tag="OHN27_000132"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCQ2284645.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15224..17536)
                     /gene="pbpC"
                     /locus_tag="OHN27_000133"
     CDS             complement(15224..17536)
                     /gene="pbpC"
                     /locus_tag="OHN27_000133"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCQ2284646.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17537..22495)
                     /gene="yfhM"
                     /locus_tag="OHN27_000134"
     CDS             complement(17537..22495)
                     /gene="yfhM"
                     /locus_tag="OHN27_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCQ2284647.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVLDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEESIALALKTPRNDELKWL
                     GDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAART
                     IQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYPQ
                     SAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLPA
                     GLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPVT
                     LVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22702..23547
                     /gene="sseA"
                     /locus_tag="OHN27_000135"
     CDS             22702..23547
                     /gene="sseA"
                     /locus_tag="OHN27_000135"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCQ2284648.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(24365..25141)
                     /gene="sseB"
                     /locus_tag="OHN27_000136"
     CDS             complement(24365..25141)
                     /gene="sseB"
                     /locus_tag="OHN27_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCQ2284649.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25283..26566)
                     /gene="pepB"
                     /locus_tag="OHN27_000137"
     CDS             complement(25283..26566)
                     /gene="pepB"
                     /locus_tag="OHN27_000137"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCQ2284650.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVVWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26625..26825)
                     /gene="iscX"
                     /locus_tag="OHN27_000138"
     CDS             complement(26625..26825)
                     /gene="iscX"
                     /locus_tag="OHN27_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCQ2284651.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26837..27172)
                     /gene="fdx"
                     /locus_tag="OHN27_000139"
     CDS             complement(26837..27172)
                     /gene="fdx"
                     /locus_tag="OHN27_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCQ2284652.1"
                     /translation="MPKIVILPHQELCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27174..29024)
                     /gene="hscA"
                     /locus_tag="OHN27_000140"
     CDS             complement(27174..29024)
                     /gene="hscA"
                     /locus_tag="OHN27_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCQ2284653.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(29041..29556)
                     /gene="hscB"
                     /locus_tag="OHN27_000141"
     CDS             complement(29041..29556)
                     /gene="hscB"
                     /locus_tag="OHN27_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCQ2284654.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEVWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29652..29975)
                     /gene="iscA"
                     /locus_tag="OHN27_000142"
     CDS             complement(29652..29975)
                     /gene="iscA"
                     /locus_tag="OHN27_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCQ2284655.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29992..30378)
                     /gene="iscU"
                     /locus_tag="OHN27_000143"
     CDS             complement(29992..30378)
                     /gene="iscU"
                     /locus_tag="OHN27_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCQ2284656.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30406..31620)
                     /gene="iscS"
                     /locus_tag="OHN27_000144"
     CDS             complement(30406..31620)
                     /gene="iscS"
                     /locus_tag="OHN27_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCQ2284657.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31732..32220)
                     /gene="iscR"
                     /locus_tag="OHN27_000145"
     CDS             complement(31732..32220)
                     /gene="iscR"
                     /locus_tag="OHN27_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCQ2284658.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32581..33321)
                     /gene="trmJ"
                     /locus_tag="OHN27_000146"
     CDS             complement(32581..33321)
                     /gene="trmJ"
                     /locus_tag="OHN27_000146"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCQ2284659.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33440..34243
                     /gene="suhB"
                     /locus_tag="OHN27_000147"
     CDS             33440..34243
                     /gene="suhB"
                     /locus_tag="OHN27_000147"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCQ2284660.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34388..35242
                     /locus_tag="OHN27_000148"
     CDS             34388..35242
                     /locus_tag="OHN27_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2284661.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35433..36713
                     /gene="csiE"
                     /locus_tag="OHN27_000149"
     CDS             35433..36713
                     /gene="csiE"
                     /locus_tag="OHN27_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCQ2284662.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGMTPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36705..37844)
                     /locus_tag="OHN27_000150"
     CDS             complement(36705..37844)
                     /locus_tag="OHN27_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCQ2284663.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(38004..38894)
                     /gene="hcaR"
                     /locus_tag="OHN27_000151"
     CDS             complement(38004..38894)
                     /gene="hcaR"
                     /locus_tag="OHN27_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCQ2284664.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            39030..40391
                     /locus_tag="OHN27_000152"
     CDS             39030..40391
                     /locus_tag="OHN27_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCQ2284665.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40388..40906
                     /locus_tag="OHN27_000153"
     CDS             40388..40906
                     /locus_tag="OHN27_000153"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCQ2284666.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40906..41226
                     /locus_tag="OHN27_000154"
     CDS             40906..41226
                     /locus_tag="OHN27_000154"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCQ2284667.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41223..42035
                     /gene="hcaB"
                     /locus_tag="OHN27_000155"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41223..42035
                     /gene="hcaB"
                     /locus_tag="OHN27_000155"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCQ2284668.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            42045..43247
                     /locus_tag="OHN27_000156"
     CDS             42045..43247
                     /locus_tag="OHN27_000156"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCQ2284669.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43344..43766
                     /locus_tag="OHN27_000157"
     CDS             43344..43766
                     /locus_tag="OHN27_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCQ2284670.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43814..44686)
                     /locus_tag="OHN27_000158"
     CDS             complement(43814..44686)
                     /locus_tag="OHN27_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCQ2284671.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44698..45792)
                     /locus_tag="OHN27_000159"
     CDS             complement(44698..45792)
                     /locus_tag="OHN27_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCQ2284672.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45825..46823)
                     /locus_tag="OHN27_000160"
     CDS             complement(45825..46823)
                     /locus_tag="OHN27_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2284673.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANIFLTQRSSKAKR"
     gene            complement(46848..48359)
                     /locus_tag="OHN27_000161"
     CDS             complement(46848..48359)
                     /locus_tag="OHN27_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCQ2284674.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48382..49365)
                     /locus_tag="OHN27_000162"
     CDS             complement(48382..49365)
                     /locus_tag="OHN27_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCQ2284675.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49462..52743)
                     /locus_tag="OHN27_000163"
     CDS             complement(49462..52743)
                     /locus_tag="OHN27_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCQ2284676.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQHSLRTCPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52861..54054
                     /locus_tag="OHN27_000164"
     CDS             52861..54054
                     /locus_tag="OHN27_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCQ2284677.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESHTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDHTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54252..55505)
                     /gene="glyA"
                     /locus_tag="OHN27_000165"
     CDS             complement(54252..55505)
                     /gene="glyA"
                     /locus_tag="OHN27_000165"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCQ2284678.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55833..57023
                     /gene="hmpA"
                     /locus_tag="OHN27_000166"
     CDS             55833..57023
                     /gene="hmpA"
                     /locus_tag="OHN27_000166"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCQ2284679.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(57068..57406)
                     /gene="glnB"
                     /locus_tag="OHN27_000167"
     CDS             complement(57068..57406)
                     /gene="glnB"
                     /locus_tag="OHN27_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCQ2284680.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57467..58801)
                     /gene="glrR"
                     /locus_tag="OHN27_000168"
     CDS             complement(57467..58801)
                     /gene="glrR"
                     /locus_tag="OHN27_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCQ2284681.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58791..59504)
                     /gene="qseG"
                     /locus_tag="OHN27_000169"
     CDS             complement(58791..59504)
                     /gene="qseG"
                     /locus_tag="OHN27_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCQ2284682.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(59669..61096)
                     /gene="qseE"
                     /locus_tag="OHN27_000170"
     CDS             complement(59669..61096)
                     /gene="qseE"
                     /locus_tag="OHN27_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCQ2284683.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61672..65559)
                     /gene="purL"
                     /locus_tag="OHN27_000171"
                     /gene_synonym="purI"
     CDS             complement(61672..65559)
                     /gene="purL"
                     /locus_tag="OHN27_000171"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCQ2284684.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65817..67373
                     /gene="mltF"
                     /locus_tag="OHN27_000172"
     CDS             65817..67373
                     /gene="mltF"
                     /locus_tag="OHN27_000172"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCQ2284685.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67370..67906)
                     /gene="tadA"
                     /locus_tag="OHN27_000173"
     CDS             complement(67370..67906)
                     /gene="tadA"
                     /locus_tag="OHN27_000173"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCQ2284686.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(67931..68566)
                     /gene="yfhb"
                     /locus_tag="OHN27_000174"
     CDS             complement(67931..68566)
                     /gene="yfhb"
                     /locus_tag="OHN27_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCQ2284687.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68775..69623
                     /locus_tag="OHN27_000175"
     CDS             68775..69623
                     /locus_tag="OHN27_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCQ2284688.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69679..69939
                     /gene="yfhL"
                     /locus_tag="OHN27_000176"
     CDS             69679..69939
                     /gene="yfhL"
                     /locus_tag="OHN27_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCQ2284689.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(70133..70213)
                     /gene="shoB"
                     /locus_tag="OHN27_000177"
     CDS             complement(70133..70213)
                     /gene="shoB"
                     /locus_tag="OHN27_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCQ2284690.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70634..71014)
                     /gene="acpS"
                     /locus_tag="OHN27_000178"
     CDS             complement(70634..71014)
                     /gene="acpS"
                     /locus_tag="OHN27_000178"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCQ2284691.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGDRLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(71014..71745)
                     /gene="pdxJ"
                     /locus_tag="OHN27_000179"
     CDS             complement(71014..71745)
                     /gene="pdxJ"
                     /locus_tag="OHN27_000179"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCQ2284692.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71757..72485)
                     /gene="recO"
                     /locus_tag="OHN27_000180"
     CDS             complement(71757..72485)
                     /gene="recO"
                     /locus_tag="OHN27_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCQ2284693.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72497..73402)
                     /gene="era"
                     /locus_tag="OHN27_000181"
     CDS             complement(72497..73402)
                     /gene="era"
                     /locus_tag="OHN27_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCQ2284694.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(73399..74079)
                     /gene="rnc"
                     /locus_tag="OHN27_000182"
     CDS             complement(73399..74079)
                     /gene="rnc"
                     /locus_tag="OHN27_000182"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCQ2284695.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(74351..75325)
                     /gene="lepB"
                     /locus_tag="OHN27_000183"
     CDS             complement(74351..75325)
                     /gene="lepB"
                     /locus_tag="OHN27_000183"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCQ2284696.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGLRLSRIGGIH"
     gene            complement(75341..77140)
                     /gene="lepA"
                     /locus_tag="OHN27_000184"
     CDS             complement(75341..77140)
                     /gene="lepA"
                     /locus_tag="OHN27_000184"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCQ2284697.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(77338..77817)
                     /gene="rseC"
                     /locus_tag="OHN27_000185"
     CDS             complement(77338..77817)
                     /gene="rseC"
                     /locus_tag="OHN27_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCQ2284698.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77814..78770)
                     /gene="rseB"
                     /locus_tag="OHN27_000186"
     CDS             complement(77814..78770)
                     /gene="rseB"
                     /locus_tag="OHN27_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCQ2284699.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78770..79420)
                     /gene="rseA"
                     /locus_tag="OHN27_000187"
     CDS             complement(78770..79420)
                     /gene="rseA"
                     /locus_tag="OHN27_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCQ2284700.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79453..80028)
                     /gene="rpoE"
                     /locus_tag="OHN27_000188"
     CDS             complement(79453..80028)
                     /gene="rpoE"
                     /locus_tag="OHN27_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCQ2284701.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(80025..80180)
                     /gene="rseD"
                     /locus_tag="OHN27_000189"
     CDS             complement(80025..80180)
                     /gene="rseD"
                     /locus_tag="OHN27_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCQ2284702.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80436..82058
                     /gene="nadB"
                     /locus_tag="OHN27_000190"
     CDS             80436..82058
                     /gene="nadB"
                     /locus_tag="OHN27_000190"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCQ2284703.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(82043..82780)
                     /gene="trmN"
                     /locus_tag="OHN27_000191"
     CDS             complement(82043..82780)
                     /gene="trmN"
                     /locus_tag="OHN27_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCQ2284704.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82912..84246
                     /gene="srmB"
                     /locus_tag="OHN27_000192"
     CDS             82912..84246
                     /gene="srmB"
                     /locus_tag="OHN27_000192"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCQ2284705.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(84455..85336)
                     /locus_tag="OHN27_000193"
     CDS             complement(84455..85336)
                     /locus_tag="OHN27_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2284706.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            85439..86026
                     /gene="eamB"
                     /locus_tag="OHN27_000194"
     CDS             85439..86026
                     /gene="eamB"
                     /locus_tag="OHN27_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCQ2284707.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(86082..86465)
                     /gene="grcA"
                     /locus_tag="OHN27_000195"
     CDS             complement(86082..86465)
                     /gene="grcA"
                     /locus_tag="OHN27_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCQ2284708.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            86770..87459
                     /gene="ung"
                     /locus_tag="OHN27_000196"
     CDS             86770..87459
                     /gene="ung"
                     /locus_tag="OHN27_000196"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCQ2284709.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(87507..88544)
                     /locus_tag="OHN27_000197"
     CDS             complement(87507..88544)
                     /locus_tag="OHN27_000197"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCQ2284710.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            88751..89170
                     /gene="trxC"
                     /locus_tag="OHN27_000198"
     CDS             88751..89170
                     /gene="trxC"
                     /locus_tag="OHN27_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCQ2284711.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            89239..89937
                     /gene="tapT"
                     /locus_tag="OHN27_000199"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             89239..89937
                     /gene="tapT"
                     /locus_tag="OHN27_000199"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCQ2284712.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            89969..92629
                     /gene="pat"
                     /locus_tag="OHN27_000200"
     CDS             89969..92629
                     /gene="pat"
                     /locus_tag="OHN27_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCQ2284713.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            92743..94098
                     /gene="pssA"
                     /locus_tag="OHN27_000201"
     CDS             92743..94098
                     /gene="pssA"
                     /locus_tag="OHN27_000201"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCQ2284714.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            94144..94467
                     /locus_tag="OHN27_000202"
     CDS             94144..94467
                     /locus_tag="OHN27_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCQ2284715.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(94464..95762)
                     /gene="kgtP"
                     /locus_tag="OHN27_000203"
     CDS             complement(94464..95762)
                     /gene="kgtP"
                     /locus_tag="OHN27_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCQ2284716.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(96087..96202)
                     /gene="rrf"
                     /locus_tag="OHN27_000204"
     rRNA            complement(96087..96202)
                     /gene="rrf"
                     /locus_tag="OHN27_000204"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(96297..>96406)
                     /locus_tag="OHN27_000205"
     rRNA            complement(96297..>96406)
                     /locus_tag="OHN27_000205"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT        23008 a        26255 c        24196 g        22947 t
ORIGIN      
        1 atagtgatga aatttaaaaa atgtcttctg cctgtggcaa tgttagcgtc attcactctg
       61 gcaggatgcc agtcaaatgc tgacgatcat gctgccgatg tttatcaaac cgatcaactg
      121 aataccaaac aagaaactaa aaccgttaat attatttcca ttcttcccgc aaaagttgcc
      181 gtagacaact cccaaaataa acggaacgca caagccttcg gcgcgcttat tggcgcagtc
      241 gctggcggtg ttatcggcca caacgtcggg tctggcagca attccggaac gacggcaggt
      301 gcagttggcg gcggagctgt aggcgcggca gcgggttcta tggtgaatga taaaacctta
      361 gtggaaggtg tttctttaac ctataaggaa ggcaccaaag tgtatacctc tacccaggtg
      421 ggtaaagagt gccagtttac gacaggttta gccgttgtta ttaccacgac gtataacgaa
      481 acgcgtattc agccaaatac caaatgtcct gaaaagagct aataatcagg aggagtcatg
      541 aagaaagttt ttctttgcgc catcttagcc tccttaagct atccggctat cgcctcatca
      601 ttgcaggatc aactctctgc tgtcgcagaa gcggaacagc aaggtaaaaa tgaagagcaa
      661 aggcagcatg acgaatgggt cgcggagcgc aacagggaaa tccagcaaga gaagcaacgt
      721 cgcgcaaatg cccaggccgc cgctaacaaa agagcggcaa cggcagcggc aaataagaaa
      781 gctcgtcagg ataaactgga cgccgaagcc tctgcggaca aaaaacgcga tcaaagttat
      841 gaagatgagc tacgcagctt agagattcag aaacaaaaac tggcgctggc gaaagaagaa
      901 gcccgcgtta agcgagaaaa cgaatttatc gatcaggaac tgaagcacaa agctgcgcaa
      961 accgatgtgg tgcaatctga agctgacgcc aacagaaata tgactgaagg cggtcgcgat
     1021 ctgatgaaaa gcgtgggcaa agcagaagag aacaaatcgg acagctggtt taattaatcg
     1081 atgttagtaa cttcaatcct ataattcttg aagataaaaa accctctgta gtaacagagg
     1141 gttttgttca ttcatagtgc agggtcaaat cattcccact caatggtagc tggcggcttg
     1201 ccgctgatgt catacaccac gcgggaaata ccgttcactt cattgataat gcggttggaa
     1261 acgcgaccga ggaaatcgta cggcagatgc gcccagtgtg cggtcataaa gtcgatggtt
     1321 tcgacagcac gcagagagac aacccagtca tacttacgac catcgcccat tacgccaacg
     1381 gaacgtaccg gcaggaacac agtgaacgcc tggctgactt tgtcgtacag gtccgcttta
     1441 cgcagttctt caatgaagat ggcgtcagca cggcgcagca ggtcacagta ctctttcttc
     1501 acttcaccca gcacacgaac gccaaggcct ggtcccggga atgggtgacg gtacaacatg
     1561 tcgtacggca ggcccagctc cagaccaatc ttacgcactt cgtctttgaa cagctctttc
     1621 agcggttcaa ccaggcccat cttcatctct ttcggcaggc cgcccacgtt gtggtgagat
     1681 ttgatgacgt gtgctttacc ggttgcagac gccgcagatt cgataacgtc agggtagatg
     1741 gtgccctgcg ccagccactt cacgtcttcc agtttcagcg cttcttcatc gaatacttca
     1801 acgaaaacgc gaccgatgat tttacgtttt gcttccggat cgttttcgcc agccagcgct
     1861 gacaggaagc gatcttctgc cggaacgtga acaatgttca gaccaaagtg atcgccaaac
     1921 atatccagaa cctgctctgc ttcgttgaga cgcagcaggc cgttgtcgac gaatacgcaa
     1981 gtcaggtttt taccgatagc gcggtgcagt agcattgcgg ttacggagga atccacgcca
     2041 ccagagaggc cgaggatgac tttatcgtcg cctacctgct cacggatgcg ggctacagca
     2101 tcgtcgataa ttttcgctgg cgtccacagg gcttcacact ggcagatatc acgcacaaaa
     2161 cgctccagca tgcgcatacc ctggcgggtg tgagtcactt ccgggtggaa ctgtacgcca
     2221 tagaagcgtt tttcttcgtt agccataatg gcaaacgggc agctttcggt gctggctacg
     2281 gtgacgaagt cggacgggat agcggtaact ttatcgccgt ggctcatcca gacatcgagc
     2341 agcggtttac cgtctgcggt cagcgcatct tcgataccgc gaaccagtgc gctgtcgttt
     2401 acgacttcaa cctgcgcgta gccaaattca cgttcgttag aggcttcaac gtgaccgccc
     2461 aactgcatcg ccatggtctg cataccatag caaacgccga ataccggtac gcctgcttca
     2521 aagacatact gcggcgcacg cggactgttt tcttcagtag tactttccgg gccgccggaa
     2581 agaataatgc cgcttggatt gaagtcacga atttgtgctt ctgtcacatc ccacgcccac
     2641 agttcgcagt aaacgcccag ctcacgcacg cggcgcgcaa ccagttgagt atactgggaa
     2701 ccgaagtcca gaatgaggat gcgatgctta tgaatgtttt ccgtcattga cgcttattcc
     2761 gaggcaagtg aaacagataa tataaatcgc ccgacatgaa gtcgggcgaa gagaatcagg
     2821 agcccagacg gtagttcggg gactctttag taatggtcac gtcgtgaacg tggctttcct
     2881 gaatgcccgc accgctgata cgtacaaact ccgctttagt acgcagttcg tcgatagtac
     2941 cacagccggt caggcccata caggagcgca ggccacccat ctgctggtga atgatctctt
     3001 tcaggcgacc tttataagct acgcgacctt cgataccttc cggcaccagt ttgtcggcag
     3061 cgttatcgct ctggaaataa cggtcagagg aacctttgga catcgcgccc agggaaccca
     3121 taccacggta agatttgtaa gaacggccct ggtagagttc gatttcaccc ggagattctt
     3181 cagtacccgc cagcatggaa cccaccatta ccgcgcttgc gccagcggcg atagctttgg
     3241 cgatgtcgcc ggagaagcga ataccaccat ctgcgataac cggaatacct gtgccttcca
     3301 gcgcttcaac tgcgtcagca acggcagtga tctgcggaac acctacgcca gtaacgatac
     3361 gagtagtaca gatagaacca gggccgatac cgactttaac cgcgctgcaa ccagcttctg
     3421 ccaaagcgcg tgcacctgca gctgttgcca cgttgccgcc gatgatttgc agatccggat
     3481 atttagcacg ggtttcacgg atacgctgca aaacgccttc tgagtgaccg tgggaggagt
     3541 cgatcagcag aacgtcaacg ccagcggcaa ccagcgcgtc aacacgctct tcgttacccg
     3601 cacctgcgcc aactgcagca ccaacacgca gacggccttg ctcgtcttta caggcgttcg
     3661 gtttacgttc cgctttctgg aaatctttca cggtgatcat gccgatcagg tggaattcgt
     3721 catcaaccac cagcgctttt tcaacgcgtt tttcgtgcat ttttgccagc accacttcac
     3781 gggcttcacc ttcacgcacg gtgaccagac gctctttcgg cgtcatgtaa acgctaaccg
     3841 gctggttcag gtcggtaaca aaacgcacgt cacgaccggt gataataccc accagttcgt
     3901 tttcttcggt aacgaccgga tagcccgcaa aaccgttacg ctcggtcagt tctttcactt
     3961 cgcgcagcgt cgtggttggc agaacagtct gcggatcagt caccacacca gattcgtgtt
     4021 ttttcacacg gcgaacttct tctgcctggc gttcaatgga catgtttttg tggataaagc
     4081 cgataccgcc ttcctgagcc agagcaatag ccaggcgcgc ttccgttacg gtatccattg
     4141 ctgcggaaag catagggata ttcagacgaa tagttttcgt cagctgggtg ctgaggtcag
     4201 cagtattcgg cagaacggta gaatgagcag gaacgaggag aacgtcgtca aacgtcagag
     4261 cttctttagc gatacgtagc atgggcaata tctcgaccag agtggttaat aaatattgcc
     4321 gcggcattat acagagcgta accgattgca tctacccctt tttgcaaaaa atgcttgcta
     4381 tccccggagg gcgggttact atcgactgaa taacctgctg atttagaatt tgatctcgct
     4441 cacatgttac cttctcaatc ccctgcaatt tttaccgtta gtcgcctgaa tcaaacggtt
     4501 cgtctgctgc ttgagcatga gatgggacag gtttggatca gcggcgaaat ctctaatttc
     4561 acacaaccgg cttccggtca ctggtacttt acactcaaag acgacaccgc ccaggtacgc
     4621 tgcgcgatgt tccgcaacag caaccgccgg gtgaccttcc gcccacagca tgggcaacaa
     4681 gttttagttc gcgccaatat tacgctctac gagccgcgcg gcgactacca gataatcgtt
     4741 gagagtatgc agccggccgg tgaagggctg ctgcaacaga agtacgaaca gctcaaagcg
     4801 aagttgcagg ctgaaggttt gttcgatcag caatacaaaa aaccacttcc ctcccctgcg
     4861 cattgcgttg gtgtgatcac ctcaaaaacc ggtgctgcgc tacatgatat tttgcatgtg
     4921 ttaaaacgtc gcgatccttc tctaccggtg atcatctacc ccaccgccgt tcagggcgat
     4981 gacgcgccgg ggcaaattgt tcgcgccatt gaactggcga atcagcgcaa tgagtgcgac
     5041 gtgttgatcg ttgggcgcgg cggcggttcg ctggaagatt tatggagttt taacgacgaa
     5101 cgcgtagcgc gggctatttt tgccagccgc attccggtcg tcagcgccgt cgggcatgag
     5161 acggatgtga ccattgccga ttttgttgcc gatctgcgtg cgccaacgcc gtctgccgcc
     5221 gctgaagtag taagccgtaa tcagcaagag ttactgcgcc aggtgcaatc ggcccgtcaa
     5281 cggctggaga tggcgatgga ttattatctc gccaaccgta cgcgtcgctt tacgcagatt
     5341 catcatcgct tgcagcagca gcatccgcag ctccggctgg cacgccagca aaccatgctt
     5401 gaacgcctga aaaaacggat gagctttgcg ctggaaaatc agcttaagcg tgccgggcaa
     5461 cagcagcagc gattaacaca gcggctgaat cagcaaaatc cacagccgaa gattcatcgc
     5521 gcgcaaacgc gcattcagca actggaatat cgtttagcag aaatcctgcg cgcacagctt
     5581 agcgccacgc gtgaacgttt cggtaatgca gtaacgcacc tcgaagccgt aagcccactg
     5641 tcaacgctgg cgcgtggata cagcgttact actgctactg acggcaatgt actgaaaaaa
     5701 gtgaagcaag ttaaagcggg tgaaatgcta accacacgtc tggaagacgg ctggatagaa
     5761 agtgaagtaa aaaacatcca gccggtaaaa aaatcgcgta aaaaggttca ttaagccagc
     5821 acgaattcaa cgcgtttttt cgatatcaag ccatgcccgt gctgacagaa gtaatctact
     5881 gcaccgcagg cttttaacac ctgcaacggt tgatggcagt ctggacaaag agctttcatt
     5941 tcaataactt ccccgcagga tgggcaatgc gcatggccgt tatcctgatc aagcacatgc
     6001 tgacattgtg gacaatgcag ttccatatga attcctccgg tagccacccc tctacattca
     6061 tagagggaat ggcagataaa atacttacgg ataattattt atttttcttg atgtgcttca
     6121 tcagacgctt acgtttacgc atctgggttg gcgtcagggt gttacgcttg ttcgcatacg
     6181 ggttttcccc ttctttgaac tgaatacgaa tcggcgatcc cattacgtcc agcgatttgc
     6241 ggaagtagtt catcaagtaa cgcttgtagg aatcaggcag gtctttcacc tgattaccgt
     6301 gaatcaccac aatcggcggg ttataaccac cggcgtgggc atatttcagc ttcacacgac
     6361 gaccgcgtac aagcggcggt tggtgatctt caacagccat cgtcatgatg cgcgtcagca
     6421 tagaggtccc cacacgacgg gtggagctgt catacgcttc acgtactgat tcaaacaagt
     6481 taccaacacc actgccgtgc aaggcagaga taaagtgcac acgagcaaaa tcgataaagc
     6541 ccagacggaa gtccagcgtt tctttcacct gctctttcac ttcctgactc aggccatccc
     6601 acttattcac cacaatgaca agtgagcgcc cactattgag aataaagccc agcagcgaga
     6661 gatcctgatc ggaaatacct tcgcgcgcat caatcactaa catcaccacg ttggcatctt
     6721 caatggcctg caacgttttg attacggaga atttctctac agcatcggtg attttaccgc
     6781 gtttacgtac gccagcggtg tcaatgagca catactcacg tccatcgcgt tccattggaa
     6841 tgtagatact gtcacgcgtc gtaccaggca tgtcataaac aacaacgcgc tcttcaccaa
     6901 gaatacggtt agtgagtgta gacttaccta cgttcggacg acccacaatc gccagtttga
     6961 tcggcagact ttgcgggtcg aagtcgtctt cctcttcttc ttcgccgttc tcttccgctt
     7021 caaattgcgc ccagtattca gcgtcttcgt cgacttcctc ttgcggtgcg agatcttcca
     7081 tccacggcag cagcacatgc tccagcagac ttaatacgcc acgaccgtga gacgcggcga
     7141 tcgggtagat ttcacctaaa ccaagcgagt agaaatcaac cacggcctga tcgggatcca
     7201 gaccgtcagt tttgtttgcc accaggaagg tcggtttttc acgggagcgc agatgtttgg
     7261 caatcgcttc atctgccggc atcaggcccg cgcgcgcatc caccataaac agtacgacgt
     7321 ccgcttcttc aatcgccagc agcgactgtt ccgccatgcg ggtttctaca ccgtcttctg
     7381 tgccatcaat cccgccggta tcaatacaga taaactcacg gccttcaatt tccgcacgac
     7441 cgtacttacg gtcacgagtc agacccggga aatccgcaac cagcgcatct cgggtgcgag
     7501 ttagacggtt aaataacgtg gattttccta cgttagggcg cccgacaagc gcgaccacag
     7561 gtaccatgtt taaagcctca tttttataaa tcatcagaca acgcacgcta tattcgcgtc
     7621 gttgttaaaa acaggaaaac ggcccctgtc caggagccgt tttcaaagtg aacgacagag
     7681 acgattaacg tgtaatagag tacacggttc cgtcttttgc ctggatcagc agtttgccgt
     7741 cagcggcaac cggttcagtc tggaaaccgg aactatcaac tttttgctgg gcaacgaaac
     7801 gaccatcttc gacgttaatc cagtgcagat aaccttcact gtcacctacc accaggttgc
     7861 cattatacag caccggagaa gtcagcaggc gatgcaacag atcgctttgt gtccacagcg
     7921 taacgccgcc atcaatggtc aacgccatca ccctgtcatt ttgatcgacc agatagatgc
     7981 gattgccgtc gacgatgaaa tcattcaccg aacccagttc gcgtttccac ataatctgac
     8041 cactgcgcag atcaagcgcc gtcaggttac cattataggc cagcgcgaaa acaacgccgt
     8101 taacaacgac gggagtcgtg tcaacatcgc tcagacggtc aatttcggta gaaccggtcg
     8161 cctgggaaat acgctgctgc caaatcatct ggccctgttc catcagcact gcgctgacgc
     8221 gaccattatc gccacccacg acggccgcac caaaagccgt tgccggcgca gactcgccac
     8281 gcaaagagag cgaaggcata tcgaggttaa ctgtccattt gacagcgccg tcagcttcgt
     8341 tcagcgcttg taactgaccg ttactggtgt ggattaacac cagaccgtcg ctgaccaccg
     8401 ggcgcgaaag tgcttcaccc gcgactttag tttgccatgc cacagtacca tcgctggtat
     8461 tcagcgcgta aacctgcgcc ttttcgctgc caatgtagac atgcccacca gacacggtca
     8521 caccgccaga aagtaatgca ggctctttag agaaccagcc atctttctcg gccaggctga
     8581 cagaccagat ttctttgcca tcatccgcat tcagcgcttt tactaaacca gcgcggtccg
     8641 ctgcatagac aacgttgtcc gccagtgccg gatgaagatt ggaatagaag ttgccaatgc
     8701 cgctaccaac ggaagtactc cacgccgtgg tcggcgtaaa ctggttttca acggttggca
     8761 atggggacat ctttaccaca tcttcttcgc tgttaaacag cgaacagccg cttaaaaggg
     8821 taacggaaag cagtcctggc agcagtaatt tacgcaattg catcgggtcc ctctcagatg
     8881 gacaaattat taattttcat ctgcatcatt tcgctcagtg ccggagtaac atcgcttttc
     8941 acgcctgctt cccatgcact acgcgcacct tgcttatcac ctttgctcag caatgcttca
     9001 ccacgcaggt cggcaacaat ggcagcccac ccttcacctt tgatggtatc aagggttttc
     9061 agcgcggcat cagcctgctt gagctgtacc tgaacgcgag caagacgcag atttatcacg
     9121 gctttgagat tttcatcgct cgtgtctgcc agcccctgtt gtaactgggc ggcagctttc
     9181 tctagttcat ttttgtcaac aaattgctgc gcaagttcca aagaagccag cgcaccataa
     9241 gtatttttat tttcagcagc aaatttttcc gccgccggga tgctatccgg tttgccttcg
     9301 ctcactgcgg taaccgcatt ttgataggca agagaagcgg agcgtgcaga atcaacctga
     9361 tggctgttcc agtagcgcca gccaatcagt gcgccaacgc ccaaaatcac cccaacagcc
     9421 agtgctttgc cattttcagc aaaaaagcgt ttaaccgctt ctacctggtc gttttcgttc
     9481 tcgtaaattt ccacgctgtc cttctccttc cttaacccag taacgtgcgc aaatgcgcgg
     9541 ctacgctatc ctgcgcaact gccgtttgct caccagagcg caaatccttc actactgctg
     9601 tgccgttagc cacttcagac tcacccagca ccacagcaac gcgggcaccc catttatcag
     9661 cacgggcaaa ctgtttctta aagttgccgc cgccgtggtt ggtcatcaat ttcacgcccg
     9721 gtaattcatc acgcagacgc tcagctaatg ccatagccgc agattgtgta tcagcacctg
     9781 aagccaccag gtatatatcg acaacaggat cggctttaaa ttccggatta acggcctgta
     9841 ctaacaatac aagacgttcg aggcccatcg caaaaccgac agccggtgtt gcacgaccgc
     9901 ccagttgttc cacaagaccg tcataacgac cgcctgcaca cacggtgccc tgggagccga
     9961 gactgttagt cacccactcg aaaacggtac ggttatagta atccagacca cgcaccagac
    10021 gctggtttac ggtgtaagcg atccccgcgc tctcaagcag tttgcacaga ccggcaaaat
    10081 gctcacgaga ttcctcgtcc agataatcac ctaatgccgg agcgtcgttg agaagcgcct
    10141 gcacttccgg attttttgaa tccagcacgc gcagcgggtt agtgtacatg cggcgtttgc
    10201 agtcttcgtc cagcttttct ttatgctgct caaggaatgc caccagcgca tcgcggtaat
    10261 tggcgcgtgc ttccagcgaa ccgatagaat tcagctcaag agttacgtgt tcggaaatac
    10321 ccagcgcgcg ccaccagcgg gcggtgagca taatcagttc agcgtcgata tccggacctt
    10381 gcagaccgaa aacttcgcag cccaactgat ggaactgacg ataacgccct ttctgcggac
    10441 gctcgtgacg gaacatcggc ccgatatacc acagacgctg ttcctgattg tacagaagac
    10501 catgctcgat gccggcgcgt acacagcccg ccgtcccttc agggcgcaga gtcaggctgt
    10561 cgccattgcg atcctcaaag gtgtacatct ctttttcaac cacgtcggtg acttcaccaa
    10621 tcgcacgttt gaatagcggg gtctgctcta caatcggcaa gcggatttca ctgtaaccgt
    10681 agctgccgag cacgtttttc agtgtgcctt caatgcgctg ccagatggcc gtttcgccag
    10741 gcaggtaatc gttcatgccg cgaatggctt gaatgttttt tgccacgttt attctctttc
    10801 tgaatataaa aatgaaccct caacgcttcc ctcaatgttt ggggagccat gcgggttcaa
    10861 tcatacacgg gaagcgaggc gcttcccatc acgttattat ttttcaacct gctgaacgtc
    10921 aattcgacgc gcttcatcca gctgactggc tttcgcacga atgcgtgctt ccagctggtc
    10981 gatcatatcg ttgttgtcca gacggtcttt gcgcacgcca tcttcataga ggccgctttt
    11041 cttgttgccg ccggtgacgc cgagtgtaga aaccagcgcc tcacctgggc cattcaccac
    11101 gcagccgata atcgaaacgt ccatcggagt gatgatatct tccaggcgtt gctccagcgc
    11161 gttaaccgta ccgataacat caaattcctg acgcgaacag gtcgggcagg cgatgaagtt
    11221 gatccctcgc gaacggatac gcagcgattt caaaatatcg aaaccgactt tgatctcttc
    11281 gaccggatcg gccgccagcg atacgcgcag cgtgtcgccg atgccttcag acagcagcag
    11341 acctaaacca atggcggatt ttactgcccc gctgcgtgca ccaccggctt cggtgatccc
    11401 cagatgcagc ggctgatcga tctgttttgc cagcaaacga taagactcaa cagcgaggaa
    11461 gacgtcagac gctttcacgc tgactttgaa ctgatcgaag ttcaggcgat cgagatgatc
    11521 aacatgacgc atggcggatt ccagcaacgc ctgcgacgtc ggttcgccat acttttcttg
    11581 cagatctttt tccagcgatc cggcgttaac gccaatacgg atcggaatgt ttttatcgcg
    11641 cgcacagtca accaccatgc gaatacgctc ttcattaccg atattgccag ggttaatacg
    11701 cagacaatcg acgccgtatt ccgctacttt cagcgcaatg cgatagtcga agtggatgtc
    11761 agccaccagc ggcacgttaa cccgctgttt gatgagtttg aacgcttctg ccgcgtccat
    11821 cgtcggtacg gagacacgga cgatatcagc gccaacgcgt tccagcgcct tgatttgatt
    11881 gaccgttgct tcgacatctg tcgtacgcgt attggtcatg gactgtacgg cgatgggagc
    11941 accatcgcca atcggcacat tcccaacgta aatacgtgtt gattttctac gttgaattgg
    12001 agcctggtta tgcatgaaaa atctcccgcg ttacccgtct gttactgcgc cggtgattgt
    12061 tcggcattga gggtcagacg cgcaacctgg ttagttctga taaaacgact cagatcgaca
    12121 ggtttccctt gatactggat ctgtactgcg gctggcgcac caattttcag tttgtacggt
    12181 gcctggccgg ttaagttcaa attaccgtct ttacgctgca taccgctaaa caatttttta
    12241 ccggtagcat cagtgacctc cagccagcaa tcggcagtaa agttcatcac cagcgcattc
    12301 ggatcagcca ccggcgtggt cacgccagcc tgatcggttg gcaacggcgc agcaccatct
    12361 ggcgttgttg ctgccgttga tgccggggtc gcggcggtat caacatttgc ctgcgaaggc
    12421 gaaacaaccg cattctgttg cggatcaaca gctggtgctg gcgcagttac ggcaggtgtt
    12481 tgcgtgttgg ttgcggtagt atccacagac gctggcggcg tggacgttgt agccgggtct
    12541 gtagttgtcg acgtatttaa cggaacactc tgcccctgct cgctattact gctcagttcc
    12601 gccgaagatt gatcggccat agtggtgatc tcttcctgct gagctttgtg gtcttgccac
    12661 caccaggcac cgctcaggcc gataaccaca aacaacacca gccaggtgaa ggtcatcagc
    12721 cagccgtcac gttttttgcg gcgtttaccg agggaaaaac tctgcatcgg cgcaactttc
    12781 gcagcccgta gtggagcctg cttttccagc cctggcagca gttcttcttc tggaatatgt
    12841 accagacgcg cataagagcg gatatatccg cgcaggaatg ttgaagcaag atcggcgggt
    12901 gccttatctt cttcaatgtc gcgtaccgtg gaaaccttca ggcaaagtcg ctcggcaacg
    12961 gcctgctgac taagtcctag ttgttcgcga gcattacgca ggcgagcgcc ggtagtaagt
    13021 gcttcatttt ggtcgtgcgt ggcttcagta ttcattcgct gcaggtacgt ttaaatgaga
    13081 attgagatgc cggtgaacca tcatgctcac ccacaccgcg aaacatccgt taagttaacc
    13141 gttatcatac agtataagac cgtctgtgtg ctcgtgacaa agcccacatt cactaaggct
    13201 aacttactgt tgcatcgtta catactgcct taaagtcagc aaaaacgcac cgttaatatt
    13261 gaccagacaa ttgcaactta atacttcatt cataaaagat tacgccacgg tacataaagt
    13321 aaccgtggcg taatggctat cagaccgctt taatgtcgat ggcttcaccc tgcatccgtt
    13381 tacgcagggt acgtttcgta cggtcgataa catcgcccgc caactgacca caggcagcat
    13441 cgatatcatc accacgggtt ttacgcacaa tagtggtgaa accgtagctc atcagcactt
    13501 ttgagaaacg gtcgatacgg ctgttcgagc tgcgtccata cggcgcgccc gggaaggggt
    13561 tccacgggat caggttgatc ttacacggcg tatctttcag cagttccgcc agttggtgcg
    13621 cgtgttcagt gccgtcgtta acgtggtcaa gcatcacata ctcaatagtg acacggccct
    13681 gattggcgtt ggatttctcc agataacggc gcaccgcagc aaggaacgtt tcgatattgt
    13741 actttttgtt gatcggcaca atttcgtcac gaatttcgtc gttcggcgcg tgcagggaaa
    13801 ttgccagtgc aacgtcgatc atatcgccca gtttatccag cgccggaact acaccggaag
    13861 tagaaagcgt gacgcgacgt ttagacaggc caaaaccgaa atcatcaagc atgatttcca
    13921 tcgccggaac gacgttgttc aggttgagca gcggctcgcc catgcccatc atcactacgt
    13981 tagtgattgg gcgctgaccg gtgacttttg ctgcgccgac gattttcgcc gcacgccaca
    14041 cctggccaat aatttccgat acccgcaggt tgcggttaaa gccctgctgg gcggtggaac
    14101 agaatttaca ctccagcgca caccccacct gcgaagagac gcagagcgtg gcacggtcgt
    14161 cttccgggat atacaccgtt tcgacgcgct gatcgccaac ggcgatcgcc catttaatgg
    14221 tgccgtcgga tgaacgctgt tcttcaacca cttccggtgc gcggatctcc gccacctctt
    14281 tcagtttgcc gcgcaacact ttgttgatgt cggtcatctc atcaaagttg tcgcagcaat
    14341 agtgatacat ccacttcatc acctgatcgg cgcggaaggg tttttcacct aaatctttaa
    14401 aaaactcccg catctgctga cggttgagat ccagcaggtt gatttttcca tctttcgtgg
    14461 tgacgttttc aggtgtgact aattgttcag acatatgcta ttccggcctc gttattacac
    14521 gttatggccc ctggagggtt gaaaaaagaa acgccccggt gagcggctgc tcgtccgggg
    14581 gcgctgcatt gtacaaattc tggcgcacgg atgccacgtt tgcacgcgac atttacgaaa
    14641 ttattaacgg gtgcgcgggc acacttcgcc ttcgccaaag aaataagcga tttcgcgagc
    14701 ggcagattcg acggaatcag aaccgtgggt accgttttcg gtcaggctgt cagcgtaatc
    14761 agcgcgcaga gtaccagcca gtgcgtttgc cggattggtc gcgcccagca gatcgcggtg
    14821 acgctgaacg gcgttttcac cttccagcac ggaaaccacg atcgggccag aggtcatgaa
    14881 ttcaaccaga ccatcaaaga acggttttcc atcgtgttca gcataaaagc cacgtgcctg
    14941 ttcaacggtc aggtgcagca ttttggtgcc gacaattttg aaccctgcag cttcaaagcg
    15001 cgcaaagata ttaccaatga cgttttttgc taccgcgttc ggtttgatga tggaaaaagt
    15061 acgttcaata gccattttta cctctgtaaa ttgttctgtt gttgtctgta ccagcgtacg
    15121 agatggcgcg gattataatg agcaacaggg ccgttgacta ttgatgaagg taacattttt
    15181 ttaaaataaa atgagtttta gcaacaatca gggacaaaac agactattgc atgacaaatt
    15241 tcactgtcgc gatttgcccc acatcatcca tcaccagcaa ttgataatca cctttatccg
    15301 tcaaatgcag ggtcacgttg cgcccgcgtt cagttaacgg ttcgccattc agaaaccacc
    15361 agcgttcacc tgcccctcca cttgattgca acggcaaagt cgcttccgct gcgcccggta
    15421 aacgtttaat aatcgcgcca tcgcgaacgc ctgtcagctg cagagggagc tgggcatcgt
    15481 gaccgtatgg cgggcaaatt gtcgaggccg gtggtaagcg cacagcgcga cgttctgatg
    15541 cgggcagcca gggttccagc ggtagcggcc agacatttat catttcttgt cgcgcttgcg
    15601 ggcaatcagc ggcaacacgt ttgccatttt catccagcca gatggggaaa cgaatgccat
    15661 taatgccttc ctgctccggc agtaatagag ttggcggctg actcccgtcc agcagccagg
    15721 ttgccaggcg gcggcgacag ttaccgtcac cttccggcaa agactgtccg cccggccagc
    15781 agataacgcc acgagtgact gagttcgggc gcgggtcttc cggcagattc gcactgcgcg
    15841 acagtaagat attattgacc tgattcaaca atggtacggc actggcaaag ccaaactgac
    15901 caacaacggg cgtgccgtcc ggtctgccag tccagatccc aatgacatag cgagcgttaa
    15961 ccccaatcgc ccaggcgtca cgatagccat agctggtgcc cgttttccat gccagtgggg
    16021 cgacgcgcgg caaggcacta tccggcaagg gttgcgcttc atcagccata atccggcgaa
    16081 tgatccacgc agcccccgag gacattaaag gccgttcaag cagcggatcg tcaggctgta
    16141 agcgcaattt gccggccttg ccgtggcgag caaacgcggt atacgctgcc gccatatctt
    16201 ccagttttgc accagcgccg ccgagaatga gcgaaagatt cggcgcagca ccgttgggca
    16261 gatacaacgg caatccaaca ttgcgtaatt ttgccgcaaa ccgtttcggt ccataggctt
    16321 ccagcacctg cacagcaggt aaattcagcg agcgcaccag cgcctcgctc atgctgatcg
    16381 ggccatgaaa accgctatca aagttacctg gtcgataatc accggtgcgc cgggggacgt
    16441 cttgcagcag tgatgccggg tggatcaagc cttcatccag cgccagacca taaacaaacg
    16501 gtttgagcac cgatcctggc gatcggatcg cattgaccat atcgacatga ccaaagcgtg
    16561 aatcatcgtt gagatcaacc gatcccaccc agccgcgaac acgcatatcg gtatgatcaa
    16621 ccacgatcat cgccagtgag ctgcgcggtg gtaatcgtcc tttccagttt tgcgccagtt
    16681 cttccagacg tcgttgaaga ccggcatcca gcgtagtaac gattttgtcg cttttgcttt
    16741 taccgagcat catgcgcgaa aacagcggtg ccagttgcgg catttgtcgg ggtgccagcc
    16801 agatgggttc ttcccttgac tcttttacct gctcacggga ccacacacct tgcacggcca
    16861 tccgttcgag cactttatta cgcgcggctt cggcacgctc cggccaacga tccgggcgaa
    16921 gacggctggg cgcttgcggc aaaaccgcca gcattgccgc ctcggaatag cttaaattcg
    16981 caggcgactt tccgagataa gcccaacttg ccgcaccgat cccctgcaac gtaccgccaa
    17041 acggagcgcg gttaagatac aaggtcagaa tttcacgctt agacagatgc cattccagtt
    17101 gcaacgcgcg ccagagctgg cgaattttgc cgccaaatgt tttggggtga ggatcaagca
    17161 gacgagcaac ctgcatagtg agcgtgctgc caccggaaat aacccgtccc gaagtgagat
    17221 cttgccatgc tgcgcgcgcc accgagaatg gattcacccc cggatgcttc cagaaccagc
    17281 gatcttcata attgatcagc gcttcaaggt aacgtggaga aacatcttcg attgttaccg
    17341 gataacgcca gatgccgtca gcatcggcga agcgccatag cggcgtacca tcctgcgcca
    17401 cgaccactcg tgcgggattg acttcatgca gcggtagagg ccagagttta tctgctcccc
    17461 acgctgcaag aaaaaggaga aatggcgcag ctgccagcgt tatccagcag ccgcgtttgg
    17521 ttaacaagcg aggcatttac ggtcggacaa tcagcagatc ttcagccgcg ccggtcgccc
    17581 gccattgggg aacatacatt gattccacca tcggttgcgg tacctgatac gttcccggcg
    17641 tcaccgcccg cgccagatac accagcgtta ccggttggta ttcatcaacg gcaaccgccg
    17701 ccacaaagcg atcgtcacgg aactcaatgt gcttaatgct cgcctgctgc atctggttca
    17761 gtaagttttg cacttcgcca ccactttgct ccaggctggc gctaccgttc gccagattct
    17821 gattttccag ttccagaccc gcaggcagca gatccacgac taacgcatcc ggcacgctgt
    17881 tactggcttt tacctgcaac cagaccagca ccagatcgcc gctacgtaac gagtccagcg
    17941 atttgctctt accatcagta ccaagaatat gacgctcgat ttgcagcaca ttgttcgccg
    18001 gtaaaggtgc ggattgcgga taaccgctgg catccacacg cagccataac ggctgatcgc
    18061 cactgtttct cacctgcaag gtggcaagtt gatcgctatt cagattgctg tttagcgttt
    18121 tctcgcctgt cagcggctca gctgagaaag aggtttgcgc ctgccattta cccggtaaat
    18181 cctgaatcgt acgggcagcc aggaacaacg cgttactttc ctgcgtcgat agccagcgtt
    18241 caccaaatgc ctgctgcgaa agcgtgttca gcaaagtgtt ctgctcatcg ggtagcagtt
    18301 tattttcttc cagcaaggag agcattaacg cgttgtcgcg cagtgggcta ccgtaatcac
    18361 ccagccattt cagctcatca ttacgcggcg ttttcagcgc cagcgcaatc gattcttcac
    18421 cacgcgtcgc atcacccatg gttttcagcg caacgccaag ttgcagcagc ggtaaaccag
    18481 aagcggcatc tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct
    18541 gacgggccag caccagcgca gcgtaagact gtacggcgaa tttactggct ttgagattat
    18601 ccgcgtacgg gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac
    18661 cccggttaat ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat
    18721 ccagcacgta agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc
    18781 cgccgttatc acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc
    18841 gtttctcatc actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa
    18901 gtgacgggaa caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact
    18961 ctttgatata acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca
    19021 gcgtaacagg cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct
    19081 gtaacgccgt accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc
    19141 actgcttatg ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct
    19201 gaatttctcc atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca
    19261 cacctggcgc taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg
    19321 cggtcagggc aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca
    19381 gacgcgaggt atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg
    19441 cggcaactat cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca
    19501 tgacgcgcaa ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt
    19561 cgttgagcgt taccggcagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt
    19621 taccaccacg tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct
    19681 gaccttcaat aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa
    19741 agaacgcctg ccacggatct ggcgtgacgt agtcagtaat attcagcaca ccactatcaa
    19801 cggcagacac cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa
    19861 ttttcacggt taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga
    19921 ggcgacggtt ttcatcgcca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg
    19981 cggagcgaga tttatcgcca ggacgtacca ccagcgtact taaatacaga tcatgacgat
    20041 tccaggtttt atcgaccgga atcgtcagat ccagcccttg agcccgaaca tcaatctctt
    20101 gccaccacag cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg
    20161 cggcgatatg caacttaatg gtgtcgccag ggcgataact ggctttatcc agtttcagcg
    20221 tgacacggtc gggtcgcact gcgccgctac cgtcgctgtt gtcctgccag ctatagccag
    20281 cccagaaacg aacactactg accgcttcat tcggcgcttt gacttccaga cgataagcgc
    20341 cccactctac cggaaaactg accttgccgg tttcgtccgc tttcagatcc agagtttgtt
    20401 cattttcgat cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt
    20461 tccagtagta atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt
    20521 tcacgccttg cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa
    20581 tgggctgttt caccgtgctg tcagtacgat aatcgtaaac cgatttcgag gcgaactgcg
    20641 gacggatccc cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg
    20701 ggcgaccgcc cgattccagc aaactaccct ggaaaataac ctgtaatggg gaatgcgttt
    20761 ccttccactg gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa
    20821 cttcatccag cgtgcgggaa agattttcgg cagctatatc gccgaattca aaaccaggta
    20881 aggctgacac agcttcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat
    20941 tagcaggtgc accatacagg tagtacccca ccacggagaa tttcacttca tctttcggcg
    21001 ttagcggggt tttctcaccg gtcagattca gcgccatgcg ctctggcata aaatcttcga
    21061 cgtggaaatc ccacatccga tactggttat cgcccgtgtt agcgcgaata tgccacatac
    21121 cggttgccgc attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac
    21181 taacgacgct cctgagtacc tgcccatcgg gtttaatcac gtctaacttg atgggttgat
    21241 tgggcaacgc tttaccgtct gcatcacgca gcaaaccatt gaggattacc gtttcacctg
    21301 ggcgataaag atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag
    21361 caatgttaaa ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac
    21421 cgtctttacg cgccaacagt aatgccgcgt ttttatcatt ttccagctgc acatgcccct
    21481 gagcgtcact ggttgcctga gtcagagtct gccctttctc atttaataaa gagacttcaa
    21541 ttccttgctg ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat
    21601 aacggtgagc tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat
    21661 cgtaacgtcc agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga
    21721 tatcgcccag cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc
    21781 gtccggtgta gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg
    21841 aattgcggta ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga
    21901 agttaacatc gacattatta acattgagcg ccattaccgg cagcccttca acgactttgc
    21961 caggcagcag cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta
    22021 tagttttttc gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa
    22081 tagtaacgat caaatcacgt ttcggttcga ggtggcgtaa acgtagctct ttaagattat
    22141 ctgacagctc ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc
    22201 gtgagaaatc ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac
    22261 catcaagttg gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg
    22321 ccagtttttg tcgctctggc gcggagagtt ttgctgaact cgcgttttct gaagaggccg
    22381 ctttagtaac ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt
    22441 cgcaccctgc cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc
    22501 atccctgcca caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt
    22561 tatgtaattc agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca
    22621 gccaaatcag cctcttttgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc
    22681 ccggcaaaaa tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc
    22741 gaacatattg atgacccgga aattcagatt atcgatgccc gcatggcgtc gcctggacag
    22801 gaggatcgta acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt
    22861 gatatcgaag cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa
    22921 acgttcgccg tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat
    22981 gacgaaggta atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta
    23041 gagaaagtgt cgattctggg gggtggactt gcaggctggc agcgcgatga tctgctgtta
    23101 gaagaaggtg cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc
    23161 gtggtgaaag taaccgatgt attattggca agccatgaaa atacggcgca aattattgat
    23221 gcccgcccgg ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc
    23281 ggacatattc ccggagcact gaatgttccg tggacggaac tggtgcgcga aggcgaacta
    23341 aaaacgaccg atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca
    23401 attatcgtca gctgcggctc tggtgtaacg gcagccgtgg ttttgttggc actcgcgacg
    23461 ctggatgtgc caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcacgggca
    23521 gatttaccgg ttgagccagt gaaataagct ttatacaggc aataaaaaac cgccgaattt
    23581 ggcggttttt tattgctggt ctggttcgcg gcctttccag caggttgact tgtgttacat
    23641 gagcaacgca ggtgcttcac agcaaaacaa tactcaccag taactctctt tttgtcaagc
    23701 aaaagagagt aattattgtt tatttagcgt attatcgaca ccggcccttt ccgccgtgtt
    23761 cggtaataaa ataacctggc ttattagtcc gaattcagac aaatataaac aaatcctgct
    23821 caaaattaaa aattctaacc ggtaaaagat attacttaaa catgtaaatt cactttcctt
    23881 taaaaaacaa aaaaccgcca aaatcaggcg gttttttgtt gctggtccgg ttcgcggcct
    23941 ttccagcagg ttgtattacc gtagtaatgc aagcgcgtct cagcggagac aatactcgcc
    24001 agtaactctc tttttgtcaa gcaaaagaga gttattattg ttctgttagt gtattatcca
    24061 ctgcggccct ttccgccgtc tcgcaaacgg gcgctggctt taggaaagga tgttccgtgg
    24121 ccgtaaatgc aggtgtttca cagcgcttgc tatcgcggca atatcgccag tggtgctgtc
    24181 gtgatgcggt cttcgcatgg accgcacaat gaagatacgg tgcttttgta tcgtacttat
    24241 tgtttctggt gcgctgttaa ccgaggtaaa taataaccgg agtctctccg gcgacaattt
    24301 actggtggtt aacaaccttc agagcagcaa gtaagcccga atgccgccct ttgggcggca
    24361 tattttagat tatccgattc tgtttaaagt cacgcaaaaa accaccccag cgacgttcat
    24421 agaatggcgc aatatgttcg gtaataaagt ggctaattcc tttttcccct tttttcacct
    24481 gacaaatatc gattggttca tcgccaggta atgtatcggt cgctacactt cccgtcgcct
    24541 gaataatttc ttcgatatca ccatcggctt caatgccaat aagtaaatta ggctgtgcct
    24601 cttcgttctc tttaattgaa caaataaaag cacgcttcac cggcttaatg gttttaaata
    24661 aggtggtgag tgaatcaatc atttgtgctg gcggctctgc gacttccgat aatatcagcg
    24721 attcaccgcc ttccaggatt tcctggctgc tcagcggatt tccctcttca ccaatcaaca
    24781 aactgatttc acgcggcata aattctttac cggttggcag tttggcatta aggaagagcg
    24841 tttcgccaag tgtcatctca aacagcgtgc gaacgggcat tacgacaaat gcctgttcgt
    24901 cttcaaccgc ctgttgaagt gcttctaacg aggtgaaaaa aggaatgacg ctggtgccgt
    24961 cttctttttc ccagtgctgt aaatcaagcg cgctatcttc aaccacagcc tcgccctgcg
    25021 ccgccgtacc aggcacccag acggtggatt ccagtagagt acggaaaaag gccgggcggt
    25081 gcgccggttc agttgctgct ttttccagca ggtcttcaag ttcgtttttt gtttcggaca
    25141 taagaaccac aattcattca acgttcgggg cgcaaatgcc tgatgcgcta cgcttatcag
    25201 gcctacaagg gattcgcaat ttgttgaatt tgcagaattt gtaggccgga taaggcgttt
    25261 acgccgcatc cggcatatta gtttacgccg ttaacagatt agcaatcgtg cgcacaccaa
    25321 gtcccgtagc gcccgcagac cactgttcaa ccggcgcttt acggtaagtc gccgagcagt
    25381 cgatatgcag ccagccttgc tgatagttct caacaaagtg cgacaggaag cccgccgccg
    25441 tgctcgcgcc tgccggatac gccgcgcttc cggtattgtt cagttcggca aagttagacg
    25501 gcagctggct gcggtggaat tccgccagcg gcagacgcca gaatggttcg ttctcttgtg
    25561 aggcactcgc cagcaaacga ccggcaagcg catcgtcaaa actgaacagc gcgtgataat
    25621 cattacccag cgcagttttc gccgccccgg tgagggtcgc cgcatcaatg atcagttccg
    25681 gtttctgcgc actggcatca atcagaccat cggcaagcac cagacgccct tcggcatcag
    25741 tgttcatcac ttcaactttt ttaccgttgc gataggtgat gatatcgccc agcttgaacg
    25801 cattgccgct aatcaggtta tccgcacagc agaggaacag cttcacgcgc ttgttcagtc
    25861 cgcgcgtaat ggcaaatgcc agcgccccgg taaccgttgc cgcgccgccc atgtccgact
    25921 tcatcgagtc cataaacgca gtctgtttga tgctgtagcc gccggagtca aaagtgatac
    25981 ctttacctac caggcacgcg tacactggcg cttctttatc gccagttggg ttgtaatcca
    26041 gcgccagcaa taccggagaa cgttctgaac cgcgtccgac tgtgtgcagc cccatataac
    26101 cttgctcacg cagatcttcg cctttggtga tccgataagt cacacgatcg cccgcgacgt
    26161 tgctgatcag atcaacagca cgctgtgcca gttgcgatgg tcccaattct tctgccggtg
    26221 cgttgatggt gtcacgcacc cagtcgatga tcatcaggcg gttatccagt tcctggcgct
    26281 gggcatcgtc cagatccggc cacaccactt tacgcgtgcc tttcggggct ttgtaacctt
    26341 gccagaatgc ccagcagcga tccgcatccc agccttcacc gcttaactga acatgcttga
    26401 tgcccagacc gtcaatcttg cgcgccgcac gctggatcag ccccagatcg tctgccccgt
    26461 tcaaatgcag ggtaatgccg tcattattaa tgctgtaagt tgctttttct ccccagcgcg
    26521 cgtcggcagg ttgggtagag agggtaatct tcatcgcttc tgtcatttta gttatcctta
    26581 ttcgtttaac agcaaaacgg gctaccccca ggcagcccgt tcattcactc ggcttcgtcc
    26641 agccagacta acaaaatcgc ttcgaggatt ttctcgttgg atgcctgcgg gtcgtcgtcg
    26701 aaatcttcca gatcgcaaat ccactgatgc atatcggtga atcgaaccgt tttcggatca
    26761 agatcgggat acgcatcgta cagtgcttcg ccaatttcgc ggctatcggt ccacttaagt
    26821 cccatactaa cctctgttaa tgctcacgcg catggttgat agtgtaacgc gggatttcga
    26881 ccactaaatc ttcgtcggtg acgcgcgcct ggcagcttaa acggctttcc ggctccagtc
    26941 cccaggcttt gtccagcatg tcgtcttcct gctctgagct ttccggcagt gagtcaaaac
    27001 cttcacgaac gatgcagtgg caggtggtgc aagcacagga tttttcacag gcgtgttcaa
    27061 tctcgatacc gttacgcaga gctgcgtcga gaatggtttc accgctatta gcttccagaa
    27121 cagcgccatc agggcagagc tcctgatgag gcaaaataac aatctttggc atattaaacc
    27181 tcgtccacgg aatggccttt cagcgcacga cgaaccgact ggtccatgcg gcgagcggcg
    27241 aaatcctggg tttgtttgtc tacgttttta atcgcttgtt cgatggcgtc aacatcatcg
    27301 ccctgcgcca cttcactcag gtgagcggca gcatcgtcaa tgacctgacg ttctgcggcg
    27361 cttaacagcg cggcatcagc agccagcgcg ccgtgcagac tttccagcac acgcgccgct
    27421 tctacttttt gttctgccag cattcgggct tttacgtcct gctcggcata gctcattgag
    27481 tctttgatca tcgaagcgat ttcgctatcg gtcagaccgt aagacggttt gacctgaata
    27541 gacgcctcaa cgccggtgga tttctccatc gccgtcacgc tcaaaagacc gtcggcatcg
    27601 acctggaacg tcacgcgaat atgcgcaccg ccagccggta gcgccggaat accacgcagc
    27661 gcaaaacgcg ccagtgagcg gcagtcctgc accagttcgc gctcaccctg cattacatgg
    27721 atagacatcg ccgtctgacc atctttaaag gtggtgaaat cctgagcgcg ggccaccgga
    27781 atagtggtat tacgcggaat cactttctcc accaggccgc ccatcgtttc gaggcccagc
    27841 gacagtggga tcacatcaag caacagcatt tcgctgtctg gcttgttacc caccagaata
    27901 tccgcctgaa tcgccgcgcc aatagcgacg actttatccg ggtcgatgga agtcagcggt
    27961 ggacgaccga aaaattcgcc tacccgttca cgcaccagcg gcacgcgagt agaaccgccc
    28021 accatcacca cttccagcac ttcgtcagct tctacacccg cgtctttaag cgcgcgacga
    28081 caagccagta aggttcgttt taccagtggc gcgatcagtt cattgaattg ttcacggctg
    28141 atttcgccct gccagcccgc aacgttaacg gtcacggagt ccgcatcgct cagcgcgatt
    28201 ttggctgcaa tggcggcatc cagcagttca cgctgaacgc ggttatcgct acgatcagga
    28261 atgcccgcct gctcgcgaat gtaatccgcc agcagatggt cgaaatcatc accaccgagc
    28321 gcggaatcac cgccggtcgc cagcacttca aacacaccgc gacttaagcg cagaatggaa
    28381 atatcaaacg tcccgccgcc gaggtcataa acggcgatca cgccttcctg accggaatcc
    28441 agcccgtagg cgatagccgc agcggtcggt tcgttaagta agcgcaggac gtgaaggccc
    28501 gccagacgcg ccgcgtcttt ggtgccctga cgctgggcat cgtcaaagta cgccggaacg
    28561 gtgataacta caccatccag ctcgcctgcc agggcttcag ttgcccgcgc cgccagtgct
    28621 ttgaggatgt ccgcagaaac gcgcaccggg ttcagcagcc ccgccgccgt ttcaatcatc
    28681 ggcaggccgt tttcgctggc ctggaattga taaggcagat gcggatagcg ttgctggata
    28741 tcagccagcg agcgccccat caggcgttta acagaactaa tggtgttggc ggtgtcaagc
    28801 gctgcgttag tacgcgcgtc ataacccacc gaatgccctt gctgttgata gtgaacaaca
    28861 gatggcagca ggtgacggcc ttcatggtcg gctaacgttt cggcctgacc gctgcgcact
    28921 gtcgccacca gcgagttggt tgtgcccagg tcaataccag ccgccagacg acgctgatgc
    28981 ggcgcagcac tcaaaccagg ttcactaatt tgtaataagg ccatgtttag cttccagaaa
    29041 ttaaaaatcg agcagttttt cttcgagttg ttcggcactg ctccgcagtt tatcgagaaa
    29101 acgcagctta cgcacggtat ccgccgccgc gtcccacacc tcgttgtcta actgttcaac
    29161 catcaactga tggcgggtat caaacatctt tttcacacgt ttgataaagc tttccagccg
    29221 cgcttcatct ttcgcctgtt cgatctcgtc cagctcttcg cgcagctcca actgttccat
    29281 caggaacgcg gtgtcgcgca cagtatgctg ctcgctggcg agatcaaaac cgtgtaaaga
    29341 aagcaaatat tccgcgcgca ttaacggatg acgcagcgtt tgccaggcct ggttaatggt
    29401 tgcagattgc tgtacggcgg cgagttgttc cgcctggctt ccgctggcga atttatcagg
    29461 atgatactga cgttgtagat cctgaaaacg caggctcagc gcctgggtat cgagttgata
    29521 acgggcaggc aagccaaaga gggtgaagta atccataaca ttctcagggc tgcgatccgc
    29581 cgcgataacg gcgaatcagg taaaacaaac cccacgcgca ggcgaccacg gtggggttat
    29641 cggtatgcgc atcaaacgtg gaagctttcg ccgcaaccac actcatcttt gacgttcggg
    29701 ttggtgaatt taaacccttc gttcaggcct tcttttacga agtccagctg cgtaccgtcc
    29761 agaaattgca ggcttttgcc atcgaccacg actttcacgc ctttgtcttc aaacacgatg
    29821 tcttccggcg tcggttcgtc aacaaattcc agtacataag ccatacctga acatccggag
    29881 gttctcacgc ccagacgcag gccaaaccct ttgccgcggt tagccagaaa ggtatttact
    29941 cgcgctgctg cactgtcgct cagtgtaatc gacataacca aacctcaact cttattttgc
    30001 ttcacgtttg cttttatagt ccgcaatggc ggctttgatc gcgtcttctg ccagaataga
    30061 acagtgaatt ttcaccggcg gcagttcaag ttcttcagca atatcggtat ttttgatcgc
    30121 ctgcgcttcg tcgagagact tccctttcac ccattcggtg accagggagc tggaagcgat
    30181 agcagaaccg cagccgtaag ttttaaaacg cgcgtcttca atgatacctt catcgttgac
    30241 tttaatctgc aacttcatca cgtcgccaca ggccggtgcc cccaccatgc cgctgccgac
    30301 gttctcgtcg ttgttgtcaa aggaacccac gttacgcgga ttctcgtaat ggtcgataac
    30361 tttttcgctg taagccatta taaattctcc tgattccgat accgattaat gatgagccca
    30421 ttcgatgctg ttcagatcca cgccctgctt gtacatttcc cacagcggag aaaggtcacg
    30481 cagacgaccg atggatttac gaactaactc gatggtgtag tcgatctctt cttcagtagt
    30541 aaaacgacct aaagagaaac ggatagagct atgtgccagc tcgtcgttca gccccagcgc
    30601 gcgcagcacg taggacggtt cgaggcttgc tgacgtacag gcggaacctg aagaaactgc
    30661 gaggtctttc agcgccataa tcagcgactc accttcaacg tagttgaagc tgacgttgag
    30721 aatgttcggc gcaccgtgtt ccaggtcacc gttcaggtaa acttcttcga tatctttgat
    30781 gccgttccac agacggttac gcaggccgcg cagacgttcc atctcggtcg ccatctcttc
    30841 ttttgcgatg cgataggcct cgcccattcc gacgatctgg tgaacaggca gagtgccgga
    30901 acgcataccg cgctcgtgac cgccgccgtg catttgcgct tcgatgcgta cgcgcggttt
    30961 acgacgtaca tacagcgcac cgataccttt cgggccatag attttgtgac cggagaaaga
    31021 catcaggtca actttcaact ggctcaggtc gataggcagt ttacccacgc tctgggttgc
    31081 atcaacgtga tagataatgc cacgagcacg gcacatttcg ccgatagccg cgatatcctg
    31141 caccacgccg atttcgttat ttacgtgcat gatggacacg aggatggtgt cgtcacgcat
    31201 cgctgcttca agttctttca ggtcgataat gccgttacgc tgcggtgcca ggtaggtgac
    31261 ttcaaaacct tcgcgctcca gctgacggca ggtatccagt accgctttgt gttcggtttt
    31321 gctggtgatg atgtgcttgc cttttttctg ataaaagttg gctgcacctt tgatcgccag
    31381 gttgtcagat tcggttgcac cagaggtaaa gacgatttca cgcggatcag cgccgaccag
    31441 atcggcaatc tgattacggg cgatatctac cgcttcttca gcctgccagc cgaaacggtg
    31501 agaacgggag gccgggttac caaaggttcc gtccatcgtc ataaactgca tcattttctc
    31561 ggcaacacgc gggtccaccg gcgtggttgc ggagtagtcg agataaatcg gtaatttcat
    31621 tgctctataa actccgtaca tcactcaatg caaggaatca ggctaccggc tggatgtacg
    31681 accgtgttta cggagtattt agcactccgg cctgattctg aattcttttt attaagcgcg
    31741 taacttaacg tcgatcgcgt cttgtgtgcg ggtgcgtggc gcgtcgtgag tatgctgacg
    31801 accagacaca tccagcactt cctggttatt aaccagttcg cctaaagtaa tgttgttgag
    31861 aaaaccggtg agacggtcgc tcaaatcacg ccacagcgcg tgggtcaggc atttatcgcc
    31921 gccctggcag ccgcctttac cctgacaacg ggtggcatct acagattcgt caacggcgct
    31981 aattacttcg ccaacggcga tgctgctggc atctttgcct aacagataac caccgcctgg
    32041 tccacgtacg ctggaaacca gaccattttt acgcagacgg gaaaacagtt gttccagata
    32101 agaaagggaa attccctgac gttcggaaat atcagccaac ggtaccgggc ccgcttcaga
    32161 gttgagcgca acgtcaagca ttgcggtcac ggcatagcgc cctttagatg tcagtctcat
    32221 gtcttacttc acctcaaact cgcccctgcc cggggttttt tattgtaaaa gtgggggtat
    32281 tgcatagcag ggtcaagtct gacattcccg agtaaattgg tcaactattt acttgactga
    32341 tttagtcggg tatttaacct tcagtgccat ttttttatcg tggcgtttgt acctgttgtc
    32401 ggatgcggcg tgaacgccat atccgccctg gggttctgta cactgtaggc ctgataagac
    32461 gcattacgcg tcgcatcagg caacggctgt cggatgcggc gtgaacgcct tatccgacct
    32521 acggttctgt tcactgtagg cctgataaga cgcattacgc gtcgcatcag gcaacggctg
    32581 ttattcggcc ttgttacctt tattctgctg ctcaatagaa gccagaatcc cgcgcaggat
    32641 attcaactcc tggctttccg ggcgcgcacg ggtaaacaga cggcgcaatt tattcatcac
    32701 ctgccccgga tggttttcac ggataaaacc ggttgccagc agcgtttgtt ccagatgacc
    32761 ataaaaacgc tccagatcat cgaccagcgg atacggcgtc tcttcatgct cgacctgctc
    32821 gccattttcc tgagttgcca gccaggccat gcgcacttca taggcaataa cctgaaccgc
    32881 catcgccagg ttcagcgagc tgtattccgg gttagccgca atcgcgacat gataatggca
    32941 tttctgcaac tcttcattgg tcaggccgac acgttcgcga ccaaaaacca gcgccaccgg
    33001 ggtatttgcc gcttcagcaa cgcttttcaa accgcattcg cgcgggtcga gcatcggcca
    33061 cggcagcgtg cgggaacgtg cgctggtgcc caccaccagg ctacaaccgg ctaacgcttc
    33121 gtcgagcgta tcgacgatgt gagcattacc aatcacatcg ctggcccccg ccgccagggc
    33181 aatcgcctgg gagtcaggtt tcaccagtgg attaaccagc cacagattgg ttaatcccat
    33241 tgttttcatg gcacgggcaa cagaacccat attgccggtg tgtgacgtct ccaccagcac
    33301 aattcgaata ttttgcagca ttgtctttct tcgtctaaag attattcgcg catcttatca
    33361 taaaacgaag acagatgccg atctcgctgc tatactctgc gccgttttcc cgttctttaa
    33421 catccagtga gagagaccga tgcatccgat gctgaacatc gccgtgcgcg cagcgcgcaa
    33481 ggcgggtaat ttaattgcca aaaactatga aaccccggac gctgtagaag cgagccagaa
    33541 aggcagtaac gatttcgtga ccaacgtaga taaagctgcc gaagcggtga ttatcgacac
    33601 gattcgtaaa tcttacccac agcacaccat catcaccgaa gaaagcggtg aacttgaagg
    33661 tactgatcag gatgttcaat gggttattga tccactggat ggcactacca actttatcaa
    33721 acgtctgccg cacttcgcgg tatctatcgc cgttcgtatc aaaggccgca ccgaagttgc
    33781 tgtggtatac gatcctatgc gtaacgaact gttcaccgcc actcgcggtc agggcgcaca
    33841 gctgaacggc taccgtctgc gcggcagcac cgctcgcgat ctcgacggta ctattctggc
    33901 gaccggcttc ccgttcaaag caaaacagta cgccactacc tacatcaaca tcgtcggcaa
    33961 actgttcaac gaatgtgcag acttccgtcg taccggttct gcggcgctgg atctggctta
    34021 cgtcgctgcg ggtcgcgttg acggtttctt tgaaatcggt ctgcgcccgt gggacttcgc
    34081 cgcaggcgag ctgctggttc gtgaagcggg cggcatcgtc agcgacttca ccggtggtca
    34141 taactacatg ctgaccggta acatcgttgc tggtaacccg cgcgttgtta aagccatgct
    34201 ggcgaacatg cgtgacgagt taagcgacgc tctgaagcgt taatgactca ggcgggtgat
    34261 atcaatcacc cgcccccgcc tttcaggcgc tattccgaaa tacttcctca ccgctttact
    34321 ttctttcgtc actctcccac catctttccc cgattaatgg atgataaaga agtcgcaagg
    34381 aaaggttatg gcactgccag tgaacaaacg cgttcccaaa attctgttta ttctctttgt
    34441 tgttgccttc tgcgtttatt tagtgccgcg cgttgccatc aacttcttct attatcccga
    34501 cgataaaatt tacggtcccg atccctggtc ggcggaatcc gtcgaattta cggctaagga
    34561 cggtactcgt ctgcaaggct ggtttatccc ttcttcgacg ggccctgctg acaacgccat
    34621 cgcaaccatc attcatgctc acggcaatgc cggaaatatg tccgcccact ggccgctggt
    34681 cagttggtta cccgagcgta atttcaacgt ttttatgttt gattatcgcg ggtttggtaa
    34741 atcaaaaggc acgccgtccc aggccggatt gctggacgat acgcaaagcg ccatcaatgt
    34801 ggtgcgccat cgcagtgatg taaacccaca acggctggtg ctgttcgggc agagtattgg
    34861 cggggcgaat attcttgctg ttattggtca gggtgatcgt gaaggcatac gtgcggtgat
    34921 cctcgactcc acatttgcct cttatgcaac catcgccaac cagatgatcc ccggcagtgg
    34981 ctacttactt gatgagagtt acagcggcga aaattacatc gccagcgtca gcccgatccc
    35041 gcttttactc attcacggta aagccgatca cgttatccca tggcagcaca gcgaaaagtt
    35101 gtatagcctg gcaaaagagc caaaacggtt gatcctaatc ccggatggcg aacacattga
    35161 tgctttttcc gatcgtcacg gcgatgttta tcgcgaacag atggtggact ttatccttag
    35221 cgcgttgaat ccgcagaact aacccatgat cgctagcacg ataatcattc acaaaaccac
    35281 cttaagacat gctaatccac tggtcagaac agtttaagat gagaaaaatt ctgtgacgct
    35341 tgccaacatt tctgatgatt agcattccct tcgtcatttc cctgagcaaa ctttagctat
    35401 tcttatcaat tatgcttatg ggagatctac agatgatgcc tacgcttgct ccaccatctg
    35461 tcctttcggc tccccagcgc cgctgccaga tcttgctgac gctctttcag ccggggttga
    35521 ccgccaccat ggcaaccttc agcgagctta atggtgtgga tgatgatatt gccagtcttg
    35581 atatcagcga aacaggacgg gagatcctgc gctatcatca actcacactg acgactggtt
    35641 atgacggtag ctaccgggtt gaaggtacag tgcttaacca acgtttgtgt ttatttcact
    35701 ggctacgacg tggtttccgt ctgtgtccgt catttattac cagccaattc acccccgccc
    35761 tgaagagtga attgaagcgg cgcggaattg cgcgtaactt ttacgacgat accaatctac
    35821 aagcgttagt gaatctctgc tcccgacggc tgcaaaaacg ctttgaatcg cgcgatattc
    35881 atttcctgtg tctgtatctg caatattgtt tgctgcagca ccacgctgga atgacgcctc
    35941 agtttaatcc gctccaacgt cgctgggcgg aatcctgcct tgaatttcag gtagcgcagg
    36001 aaattggacg ccactggcag cgtcgggcgc tccagcctgt accgcctgat gagccactgt
    36061 ttatggcact acttttttcc atgttgcggg ttcccgatcc actgcgggat gcgcatcagc
    36121 gagacagaca attgcgtcag tctatcaaac gtctggtaaa ccattttcgt gagctgggaa
    36181 atgttcgttt ttatgatgaa caggggttat gtgatcagct ttatacccac ctcgcccagg
    36241 cgttaaatcg cagtttgttt gccatcggta ttgataatac cctgccggaa gagttcgcca
    36301 gactgtaccc acgcctggtg cgcaccaccc gcgcggcgct ggccggattt gaaagtgaat
    36361 acggcgtcca tctttctgat gaggaaagtg gtctggtcgc ggtgattttc ggtgcctggc
    36421 taatgcagga aaacgatctg catgaaaaac agattattct actgaccggg aatgatagcg
    36481 agcgagaagc gcagattgag cagcagttac gcgaactaac gttactgccg ctcaacatta
    36541 agcatatgtc ggtaaaggca tttttgcaga caggcgctcc gcgtggcgcg gcactgatta
    36601 ttgcgcctta taccatgcca ttaccgctct tttcaccacc gctgatctat acggacctga
    36661 cgctgacaac acatcaacag gagcagatcc gcaaaatgct cgaatcagca tgagggaaca
    36721 actttcgggc gcaaaaacat tgccggcagc gccaccagcg ccattaccca gaacacgccg
    36781 tggcccagat attgatacag gaaaccggcg aaaacggtca tgatagcgat actgccgccc
    36841 atcgcgacgg cagagtaaac cgcctgtaaa cggatgactt cgctaccctg gcgagcagca
    36901 atataacgca tggcggccag gtggcagacc gtgaaggtgc cgcaatgcag aatttgcacc
    36961 actatcaacc acggcaacgc cgtagttgct cccataatgc cccagcgcac tacgccgcaa
    37021 atcgccgaga tcaacagcat atcgcgtgca ctacaacggc ggaaaagttt attactcagc
    37081 gcaaagataa tgacttccgc caccacgccc agcgaccaca aataccccac cgccgaggcc
    37141 gagtagccag ctgcctgcca gtaaatggcg ctaaaaccgt aataggccgc atgtgccccc
    37201 tgcaataaac aaacgcaggc cagaaagcgc cagttctggc gaaccagcgc caaccacgct
    37261 gaccaaccgg tgctctcctg ctggcggctt gccccttgtg gctgaatcgt cggacggatg
    37321 agaaagccga gcagcatgga tgccacgccc aacgtcaaca gcgcgagtat cacccgataa
    37381 tcaaacatag tgaccagttt gcccgtcagc gccgagccaa tgacaaacgc caccgagccc
    37441 cacagtcgca ctttgccgta atcaagcggg aactgctttt gccacgtatt cgccagtgca
    37501 tcggtcaacg gtaccagcgg tgagaaaaag aggttaaagc caatcatcac cagcatcagc
    37561 cacgctacgt gcgcccccgc ccagaaggcg acagcaaaga gaagtgtcag cagtgccagc
    37621 acgcgcaagg cggaaatcag gcgggaagga tcgctgacgc ggggcgcgat gagcaaactc
    37681 ccgagaaaac gggcaaccag acctgccccc aataacaggc cgatggtttc tggcgttaaa
    37741 ccaatccctt taagccagac gctccagaaa ggtagaaaaa tgccgtaact aaaaaagtat
    37801 gtgaaatagc cgagcgccaa ccagcgcgtg gattgcaaaa ccatgagtcc ctcccgtcag
    37861 gaggcgttag tctggggtta atcgaatgtt ggtgcaagtt gaaaaataag aatattcgag
    37921 cctgcttccc gatgtctgta acgccagttc acaagcacta aaaaactgag atccgcgacg
    37981 catgttgcaa aaataatgcg taattatgcc gttacgcttg ccaaacgttc ctgcacaatg
    38041 gcgatgaaat cgcgtaatgc cggcttcatc tcccctttct tccacgccat cagtaaagca
    38101 atggaaggca catttccggc aatgggacga aaaacaacct gcccggtatt aaaattattc
    38161 atataaccgg gtatcaaagt gacgcccagc cccatgccca ccagattcat ggtcaccaga
    38221 atattcgttg ccacctggac gatatttggc tggctgtttt cttgcgcaaa ccacgcttta
    38281 acgatcggcg caagcgaacc ggaatacgcc ggatcggtac tgacgaaatt cacgccatcc
    38341 agttgcgcgg cggtgatctc tttttcatgt gctaaagggt gatcaaccgg taacaccacc
    38401 actaatggtt cgtcaaaaag ctccagataa tcaatctccg ggctataaac aggatggcgc
    38461 attaagccga cgtcgagttc accacggcga attttttcct cctgttgcgt ggtgattaaa
    38521 ctcaccagct caatcaaggt gtctggctgt ctgagacgaa acatcggtaa tacttttggc
    38581 aataaattca cttccgccga tggcacaaag ccaatggtta attgtctgtc ttcctgaaca
    38641 attttccgcg cccgtaattt ggcattttcc gcttgttcga ggattgccag cgcgtcctgg
    38701 agaaaacatt ctcccgccgc cgtcaacgcg actttgcgct tatccctcac cagtaacgga
    38761 acaccgacac agttttcaag atcgcggatc tggctgctta acgaaggctg tgaggtatgc
    38821 agtttttccg ccgcacgggt aaagttcagt gcctgcgcca ctgcgacgaa atagcgtaaa
    38881 tgccgtagtt ccatcacctt ccccttgtta tcgaaaaaac gtctcagctg gtagaaatta
    38941 actatttcac atattagcaa ccaaccagca acatccttat ggcacaaaaa tagaaggtca
    39001 atacatctta tctttcagga ttaaaaaata tgaccacacc ctcagatttg aacatttacc
    39061 aactgattga tacccaaaat ggtcgggtca ctccgcgtat ttataccgac ccagatattt
    39121 accaactgga gcttgaacgt attttcggcc gttgctggtt atttctcgcc cacgaaagcc
    39181 agatcccaaa acccggtgat ttctttaaca cctacatggg ggaagatgcg gttgtcgtag
    39241 tgcgtcagaa agacggcagc attaaggcgt ttctcaacca atgccgccac cgggccatgc
    39301 gtgtgagtta tgcagattgc ggcaacactc gcgcctttac ctgtccgtat cacggctggt
    39361 cttatggcat taacggcgag ttgatcgatg taccgctgga acctcgcgcc tacccacaag
    39421 ggttgtgtaa atcccactgg ggactaaacg aagttccttg tgtggagagt tataaagggc
    39481 tgatttttgg caactgggat accagcgcac cgggcctgcg tgattacctg ggtgacattg
    39541 cctggtatct ggatggcatg ctggatcgtc gcgaaggcgg caccgaaatt gtcggcggcg
    39601 tacaaaagtg ggtgatcaac tgcaactgga aattcccggc agagcagttc gccagtgacc
    39661 agtatcatgc tctgttcagc catgcttctg ccgttcaggt attaggggcg aaagatgatg
    39721 gcagcgataa gcgcctcggt gatggacaaa ccgcccgccc ggtgtgggaa accgccaaag
    39781 atgcgctgca atttggtcag gacggtcacg gtagcggttt cttctttact gaaaaaccgg
    39841 atgctaatgt ctgggtcgat ggcgcagttt caagctatta ccgcgaaacc tatgccgaag
    39901 cagaacaacg tttaggtgaa gttcgcgccc tgcgcctggc gggtcataac aatattttcc
    39961 ccacgctttc atggctcaac ggcactgcca cgctccgcgt ctggcatccg cgcggccctg
    40021 atcaagttga agtgtgggcg ttctgtatta ctgacaaagc cgcctccgat gaagttaaag
    40081 ccgcttttga aaacagcgcc actcgtgctt ttggtcctgc tggttttctc gagcaggatg
    40141 actcggagaa ctggtgtgaa atccagaaat tgcttaaagg ccaccgcgcc cgcaacagca
    40201 aactgtgtct ggaaatgggg cttggtcagg aaaagcgtcg cgacgacggc attcctggca
    40261 ttactaacta tattttctca gaaactgccg ctcgcggaat gtaccaacgt tgggccgatc
    40321 tcctgagtag cgaaagctgg caagaagtgc tcgataaaac cgccgcttac cagcaggagg
    40381 tgatgaaatg agtgcgcaag tttcactgga gttacatcac cgcattagcc agtttctctt
    40441 tcacgaagcc agtctgctgg atgactggaa atttcgtgac tggctggcac agctcgacga
    40501 agagattcgt tacaccatgc gcaccacggt taacgcgcaa acgcgcgacc gccgcaaagg
    40561 cgttcagcca ccgacgacct ggatttttaa tgacaccaaa gaccagctgg agcggcgaat
    40621 cgcccgtctg gaaacgggca tggcctgggc agaagagccg ccgtcacgca ctcgtcactt
    40681 aatcagcaac tgccaggtaa gcgaaaccga catcccaaat gtatttgccg tgagggtgaa
    40741 ttatctactt tatcgggcac aaaaagagcg cgatgaaaca ttctatgttg gaacgcgttt
    40801 cgacaaagtt cgccgtctgg aagatgacaa ctggcgcttg ctggaacggg atatcgtcct
    40861 ggatcaagcg gtaatcactt cccataacct gagtgtactg ttctgatgaa tcgaatttat
    40921 gcgtgtcccg ttgcggatgt gccggagggt gaggctctcc ggatcgatac ctcgcccgtc
    40981 atcgccctgt tcaacgttgg cggcgagttt tatgccatta acgatcgttg cagccatggt
    41041 aatgcgtcaa tgtcagaagg gtatctggaa gatgacgcca cggtggagtg cccgctacac
    41101 gccgccagtt tttgcctgaa aacggggaaa gcgttatgcc tgcccgccac cgatccgctc
    41161 accacttatc cagtacacgt tgaaggtggt gacattttca tcgacttacc ggaggcgcag
    41221 ccatgagcga tctgcataac gagtccattt ttattaccgg cggcggatcg ggattagggc
    41281 tggcgctggt cgagcgattt atcgaagaag gcgcgcaggt tgccacgctg gaactgtcgg
    41341 cggcaaaagt cgccagtctg cgtcagcgat ttggcgaaca tattctggcg gtggaaggta
    41401 acgtgacctg ttatgccgat tatcaacgcg cggtcgatca gatcctgact cgttccggca
    41461 agctggattg ttttatcggc aatgcaggca tctgggatca caatgcctca ctggttaata
    41521 ctcccgcaga gacgctcgaa accggcttcc acgagctgtt taacgtcaac gtactcggtt
    41581 acctgctggg cgcaaaagcc tgcgctccgg cgttaatcgc cagtgaaggc agcatgattt
    41641 tcacactgtc aaatgccgcc tggtatcctg gcggcggtgg cccgctgtac accgccagta
    41701 aacatgccgc aaccggactt attcgccaac tggcttatga actggcaccg aaagtgcggg
    41761 tgaatggcgt cggcccgtgt ggtatggcca gcgacctgcg cggcccacag gcgctcgggc
    41821 aaagtgaaac ctcgataatg cagtctctga cgccggagaa aattgccgcc attttaccgc
    41881 tgcaattttt cccgcaaccg gcggatttta cggggccgta tgtgatgttg gcatcgcggc
    41941 gcaataatcg cgcattaagc ggtgtgatga tcaacgctga tgcgggttta gcgattcgcg
    42001 gcattcgcca cgtagcggct gggctggatc tttaaggaag cacgatgaaa gaaaaaacga
    42061 tcattattgt cggtggcggg caagcggcgg caatggctgc ggcctcgcta cgccagcaag
    42121 ggttcaccgg tgagctgcat ctgttttccg atgagcgaca tcttccttat gaacgacctc
    42181 cactctcgaa atccatgttg ctggaagatt ccccgcagtt acagcaggtg ttacccgcta
    42241 actggtggca ggaaaacaat gttcatctgc attccggtgt aaccatcaaa acgctgggtc
    42301 gcgacacacg agagttagtg ttaaccaacg gcgaaagctg gcactgggat cagcttttta
    42361 tagcaaccgg cgcggcagct cgaccgctgc cgttgcttga tgcactggga gaacgctgct
    42421 ttaccctacg ccatgccggt gatgccgcca gactgcgaga agttctgcag cccgaacggt
    42481 cagtcgtgat tatcggtgcc ggaactattg gtctggaact ggctgccagc gccacgcagc
    42541 gcagatgtaa ggtgacagtg attgaactgg cggcaaccgt catgggccgt aatgcaccac
    42601 cgcccgtgca acgctatctt ttacagcgcc accagcaggc tggtgtgcgc attctgctca
    42661 ataatgccat tgaacatgtg gtcgatggtg aaaaagtaga actgacgctg caaagtgggg
    42721 aaacgcttca ggctgatgtg gtgatttacg gtattggtat cagcgccaac gagcaactgg
    42781 ctcgcgaggc caaccttgat actgccaatg gcattgtcat tgatgaggct tgccgcacct
    42841 gcgatcccgc gatctttgcc ggtggcgatg tggcaatcac tcgtcttgat aatggtgcac
    42901 tacaccgctg cgaaagctgg gaaaacgcca ataaccaggc gcaaattgcc gctgccgcaa
    42961 tgttagggct accgctaccg ctactgccgc cgccgtggtt ctggagcgat cagtacagtg
    43021 ataacttaca gtttattggc gatatgcgtg gcgatgactg gctttgtcgt ggcaacccgg
    43081 aaactcagaa ggcgatttgg tttaatctgc aaaacggcgt gcttatcggt gcggtcacgc
    43141 tgaatcaggg gcgtgagatt cgcccaattc gcaaatggat ccagagcggc aaaacgtttg
    43201 atgcgaaact gctgatagat gagaacatcg cgcttaaatc actgtaacca ggataattag
    43261 cgaatatctc aatgcctagg gcgtggcgag gtgcaagagt gtgtattacg tttaaatcac
    43321 attatcttgc aaagggattg gttatgaaca cactacgtta ttttgatttt ggagctgccc
    43381 gccccgtttt gttattaatt gcccgtatcg ccgtggtctt aattttcatt atttttggtt
    43441 ttcccaaaat gatgggcttt gacggtacgg tccaatatat ggcctcgttg ggcgcgccaa
    43501 tgccgatgct ggcagcgatt attgcggtag ttatggaagt gcccgccgcg atattaatcg
    43561 tgcttggctt tttcacccgt ccgctggcgg tgctgtttat tttctacacg ctgggtacgg
    43621 cggtgattgg tcaccattac tgggatatga ccggcgatgc ggttgggcca aatatgatta
    43681 atttctggaa gaatgtcagt atcgctggcg cgttcttgct attggcaatt accgggccgg
    43741 gggcaatttc tctcgatcgg cgttaggaaa gatgccggat gcggcgtgaa cgccttatcc
    43801 ggcatttaat aaattacagc cactctaccc gcaacgacat ctctgaagtt gttgattccc
    43861 ctggtgctag cgcaatgaga tcaccccctt ccgggcgatg atgatcatcc ggcgcatggc
    43921 tcatcggttc cagacagaag aaatcaaacg catatccttt atcaaacgca gggtcggaaa
    43981 caaagatgaa ataacacggt gcaggtggcg tcgtttccat gatgatggca taaccttcct
    44041 gcggctgctc gatgcgggcc tgaccattcc atccggcaaa accattgttc acccactggc
    44101 gcggcaacgg cgcgggctgg ttaaaatcca gttcctgcgg tagctgctcg caaaactcac
    44161 ccgccagcca ctgctcccgc tccagccagt aaccgctcgc ctgtgcctga atccgcgttt
    44221 gcggcgacaa cggaaaataa ggatgccagc cggtaccaaa tggcagcgtc tctgcccctt
    44281 gattggtgac agagagcgtc accgtcagcg tatccgccgt taaatgaaac gcctgactta
    44341 cccgatagtg atagacaccg ctgcgatgtt catacaccag acacaaacta tcatcgctgt
    44401 gcgagacaca ttgccactcg cccagccagc catcgccgtg tagatagtgc gcatcccact
    44461 caacattcgg ttgcagttga tactcacgcc cctgccagac aaaacgattg ccgctcaccc
    44521 ggtttgcaaa tggcaccagc ggaaaacatg aggcatcggt tgccacaccg ctttttttac
    44581 caggacgtaa gagcggcgtc gtatcgcgcc agaagccttc gattacgccg ccctggtcag
    44641 aaacgtccag ctttagcgac ccgtgggata aggtatagat ggtcatccgc taactcctta
    44701 atccgggaag ttaatcacaa ctttcccgca tttgccgctc gccatcagcg cataggcatc
    44761 acctgcctgt tccagcgaga agcgatgggt aatggcgtta cgcggccaca gcttccagtc
    44821 cgtcagatca tgggcgcatt tttccatatg gaacagactg gtcacccagg agccgataat
    44881 ccgccgttga tggtgcatca gatcggcgct gacctcgaat tccacttttc cggtttcacc
    44941 aatgtaaacc acccgtcccc agtcagcggt ggattgcagt gccagcaagc gacctgcggc
    45001 attaccggaa caatcgagcg caacatccgc gccaccgtgg gtgagttcgg cgataatctg
    45061 cggcagacct tcggtggttg ctaaatagcc gtgatccatc acccctaact gttttgccat
    45121 cgccagacgt tccggcagca tatcaacgcc gatgatccgt tttgcaccgc gacctttcgc
    45181 cagcatcatc gccatcatgc cgaccggccc cagaccgacc accagcacgt tatcgctgcc
    45241 ggaaacttcg ccgcgcaaaa tcccttcata ggccgtgcca acgccgcaac tgataaacgc
    45301 accatcttcg tagctcagcg catccggcag gaggatcaga tctttttctt ccgccagcag
    45361 atattcggca tgaccgccgt cacgctgcca gccgtaagcc gcttttcctt tgccagtaca
    45421 agagatagga aagccgcggc ggcagttcgg gcaaaaacca cagccagaaa tgtgatacac
    45481 cagcacgcgg tcgccctctt taaaatggcg gcagccttgc cccatcgcca caatctgccc
    45541 gcatggttca tgaccgttga taaatccctg gtataacggt ttatcgggtg ccgccgccgt
    45601 ggcacggtgt tgatgataga tatagtggac atcgcttccg caaatcccgg aggatttcat
    45661 tttgatcagt acctggttaa tccccggcgt cggcaccgca acttcccgca gatcgacggt
    45721 tgaatttcct ggtaaataag ctgccagcat cgttttcata aatccctcat taacaatacg
    45781 attaattttc atccctgccg cacccgcgcc aggggcgtta aggtttagcg tttcgcttta
    45841 ctgcttcgct gggtcaggaa gatattcgcc agcaccgcca ccacgatgat gacgccgcgt
    45901 accacctgct ggaaaaagga gttaataccg agcagcacca gaccgttacc gattagcgta
    45961 atcaccagca caccaagcaa tgtaccgaac agggaaccgc gaccgccgga aagtgccgta
    46021 ccgccgacca cgaccgcagc gatgacgtca aactccagac cgtttgcggc acctgcgtta
    46081 ccggaaccga ggcgtgccgc cagcaaaatg ccggtcaccg ccgctaataa tcccgaaagg
    46141 gtaaagataa gaatgcgcac ccgacgaacg ttaataccgc acaactgcgc cgccgtggca
    46201 ttaccgccaa cggcaaaaac cgagcgcccg aaggcggttt ttcggctaat gaacacaaac
    46261 agcgcaaaca acaccatcat gatcagcgcg gatactggca caccgagaaa ttgtccgccc
    46321 agccagtcca gcacctcgtt ttcgtcaatc ggcactggca gtgcgttggt cataaacagc
    46381 cccattccgc gcagggcgct ccacagcccc agtgtggcaa cgaaacttgg tacgttaaac
    46441 acgccgcgca gcaccccggc gagcgttccc atcagcgcgc ctaacagcaa caccagcaga
    46501 caagcaatcg ccaacggaac ttcaaattgc agcaaaaatg ccaggcacac cgagacaaaa
    46561 gccaccatcg gcccaacgct gacatcaatt tcaccggaga taataatcag cgtcatcgcc
    46621 caggcggcaa tcccaatggt ggcggcatcg cgcagcacgt tcatctggtt attcaatgag
    46681 ataaagccag gcgcgttcag ggagaagacc agataaagaa tggcgattac caccagcaaa
    46741 ccgatctcat taatatggcg actgacaaat tgtttgagcg agacgctctt gccctgcggc
    46801 aatgataatg acgaagcaga catcttcagg ttcctcggta aaattcatca gtgcacagac
    46861 agaatggcgg acatcagctc atccacattg accggcgagt gaaactcctg cgagaacgta
    46921 ccgtgctgta acaacaggat gcggtcgcac accagcggca actcctccac ttcactggag
    46981 ataaacacca cgctttttcc ttcggcagcc agctcacgga caatacggta aatctgctgt
    47041 ttggcttcga tatcgacgcc gcgcgttggc tcgtcgagca acaaaatctg gctggcggca
    47101 tagacccaac gaccgatcac cactttttgc tgattgccac cagaaagcgt gccgatgggt
    47161 gtttcgctac tggcagcctt gaccgtcatc cgctgcatca cctcttcggt caggcggcgg
    47221 atggtggacc attgcagcac accgttggtg ctgatttttt gccgattggt cagcactgta
    47281 ttttcgtcaa cgcccagcca gggaatgatc cccgcttctt tgcggttttc tggcgtatag
    47341 ccaatgccgc gtttcagcat gtcgccgtaa tcggggcgca tgattttctc gccgttgata
    47401 acaatttcgc cctgttcata cgtctccagc ccaacaatcg ccttcagcaa ttcactgcgc
    47461 cctgccccca gcaggccagc aatgccaagc acttcgccac gacgtagcgt aaagctgata
    47521 tcctccagct tgggcttatg gcgtaacgca cggacttcca gtacggcctg atccacaatt
    47581 tcctgagggg ctaccggcgc aatatcaacg tgatcacgcc ctagcattag cgacacaata
    47641 tgatgcgtgg aggtgttttc gagcatcaca tcgcccgcca cctgaccatc gcgcataacg
    47701 gtggcacagg aggcaatgcg gcgaatttct tccatccggt ggctgacata aatcaccgcc
    47761 acgcccagtg ccgacatctt tttcaccgcg ctgatcacca gttcaacttc cgcactcgca
    47821 agcgaactgg taggttcatc aagaatgacc acgcgcggct cgcccttcat cacccgcgca
    47881 atttccacca gctgcttttg cgccgggctt agcgttgaaa caagttgttc aggactaacg
    47941 tcaacgccca gcgcctgtaa gcaacgttgg gcatcctgcg ccatttgcag gtaatcaatc
    48001 atgccgttgc ggcggggcca ctgaccgagg cagaggtttt ccgccaccgt cagcccttcc
    48061 accagactta attcctgata aaccgcacga acccccagtt cagcggcacg gcgagtcaac
    48121 gtagcttcgt caccttccag tcgcgtctcg ccaatccaga tatcaccgct atccgggcgt
    48181 tcgctgccgg taagcattcg aatgagagtc gatttgcccg cgccgttttt acctaacagc
    48241 gcacgaactt cgcctttatt gagcgtgaag ttaacgttat ccagcgcaac gacgccgggg
    48301 taacgcttat ttcctgccac cacttttgct accgggactg cctctgttgc cgtgaacata
    48361 gggacctctg cgaatcagcg attagggcag accatcaacg tgcgttgcca gccactgttt
    48421 accatcttcc gttttggtat agagatcgat tggcacctga atcacttttt caccgtcggc
    48481 ttgtttattg ataaccttca atgtttgcgc gaaaacagca ttgcccattt tcttaccgga
    48541 aatatccact accgctttca gcacctgatt gttttccagc tcctgagcaa tttcggttgt
    48601 catatccgaa ccgaaaacag caatttttcc ggcctgattt tgattacgta ccgcttttac
    48661 cgcgccgagt gtcgcaccgc ccgactcccc cataatggcg ttgagatccg gcgtggagat
    48721 aatcagtttt tcaccaacgg aaatcgcttt atctaaaaca gtcccttcct gattagcgac
    48781 aatttgcgcg ccgggaacgc gggattttaa tacttcttca aatcctttac gtcgctgcac
    48841 acaaacttca aaggcttcgc aattgatgac ggcaattttc ggctggtcaa ttttattggc
    48901 aataaaataa tcggcggcag cgttacccag ttttttacca aattccagtg gatcgccgac
    48961 cagatacgcc gagacatatt tatcgacacc cttttgatta atacaggtgt tgtagcaaat
    49021 caccggaata cccgcttcac tggcgcgacg aacggtacgg ctactgccat tttcagacac
    49081 tgccgataaa ataatggcat cgacattacg cgccacgagg gtatcaacaa aggtactttc
    49141 tttcgaaata tcaccctggg cgttagtttc aattaactgt acctgtactg aagaatcttt
    49201 tgccgcatcc tgaacgccct gacgcactcc agcgtaatat ccctgggtat caaggtatat
    49261 tgcaccaatg gtcatttctt tatccgcagc cctggcaaat agtgcgctac ctaatagcgt
    49321 agccattaat aatagattac gggttgttct cattttttta ggcatagtgc ctcctgtagg
    49381 ttttttatta acaacggctt attctaatta ttttgtgatg agcggcagcg catgccgctc
    49441 aaatattaca gttaataggg tttagtgaac gtagttaaaa ataaaaggca tcacggtggt
    49501 gaataatgcc gctttcggcc aggccggatt aatcgccgtc aattcagcgc gagcagattc
    49561 atattgcgca acctccccga gtcccgcgga cgccagcatc gccaccatca ggcatttttc
    49621 tttatgctgc tgttgtaaat cgccatacag cgacaacagg tcaggttgtg aaacagcaaa
    49681 gaaatccgcc tcaatactgg ttttcgccat ctcttgcgcc cactgtttca tttcactaaa
    49741 cagttgctgt gcggtttgct gttcacccag cagtcgcagc gccattcctt gccagaagag
    49801 ataatcaacc ggctgatcgt tgtaataact gtggatgtta atggtgcgat cgccggtcgc
    49861 cgccagacgt aaacaacgca tcgcttcagt ttcatcgccc tgcgcgttgg cgcatatcgc
    49921 ctgccagaac cagatgtcgt tatcagtttg ccccggtaaa cggccttcgc ttaaattctc
    49981 cggataatgc agcgcggcat gaagcagttc gctggcctgc tgcggctgtc tggcatcaag
    50041 atgctgccag gcgcgtaata actggttgag gataaactga ctggtgacct tcccttcccc
    50101 gccttcccac gggtggaatt tacgcgtggc gagaatgtcc gccgctttgt ctgcctgacc
    50161 cgtgagatgc cacaaattga gcagttctgc ggtcatgtcg tcgcgtttca gcgcaatttc
    50221 ctgattattt tccagacgcg ccagtcgttt ctccggtgtg gctccactta acttatcaag
    50281 caaatcccgt tcgaaaagca gacgtgcatc ctgcggcgca agctgataag cattatcaag
    50341 ataacgcgcg gccagctcat aatcgtgttg cttattccac gcatggatcg ctaacccgcg
    50401 ccagccgtcg gcaaactccg gcgacatctc tacgcaacgt tgccataagg caatggcttt
    50461 gttgtagcta cgtttgttgt agtagaagca ggccagtaaa tggcgagcaa accagcactc
    50521 ttcaatactc tccagcgccg ccacttcttc caacgtattc gggaaacgga caaactgcgg
    50581 gaagacatca atggcttttg cgaccagttc gccacgttcg gctttcggca gcaagctggc
    50641 ttgcaggtaa agcggcagcg tgcgctggca gtccagtgcg ttcagcatct ctgccgccag
    50701 ggtgggcatt ccccagttaa tcagttgccc ggcggtcatc agggcgttaa cgtcgcgtcc
    50761 ctgacacaga ccgcgccact gcgcgagagc tgattcgcta cgaccatcga accagttcag
    50821 ccaccacaga gtggcgttca gcggataatc gcgcagcagt ttctcgcgct gcaaacgcgc
    50881 gttgtcctga cgaccactta acaccagcag cagattatgc aggcaaagca cttcctgatt
    50941 ggttgggcag gtgcgaagac tgtgttggca aaaatccaga cccgcgtcga agtttccatt
    51001 acgcgccgcc agtcgtgcca gaccataata gccgcctgct ttactattgc cgctccagac
    51061 cgcacgccag aaatcctctt cggcttgttg atattgtccc tgacgttcgt aagcactggc
    51121 gcgaatcaaa ctcgcctgtc cgcactgcgg atttttgttc agcgcatgtg cgcgtttcag
    51181 cgcctgagtg gcatacgcca ccgcttgtgg gaaatctgcg cggttatatt ccagcatcgc
    51241 cagcgccagg ttacagcggt aatccagcgg atccagcgcc acaccgcgca ggtagtaatc
    51301 gaacggtgaa cggctggcgt gatgatattg ctccagatgc tgaccgataa accaggcttc
    51361 atctgtactg gtaatgtctt gtgctgccag tggcgctttg gcgacgtccg gcaacggcaa
    51421 ctcttgcggc tgatgttcct gataactcag tacaatattg ccgtcggcat cggagagctc
    51481 aatggtcagc ctttcaggat tgataccgtg caacacgccc tggatggcgg tcgctggtgt
    51541 caaggccacg gcatcatcaa gtaacgcgtt gcatttgccg atttcgcgga tcgccaggcg
    51601 gtatccgttc aacggagaga tggcatacag cccccactca atcccccgct cactacgctg
    51661 gagttttatc accgcatcgc gggaggcatt ttgcaccatg cccagagaat gataaggcag
    51721 gaaatactgc tcgaaacgct tctcttcgta agcatcaagc caggtaaaat caggctggtt
    51781 atcggcaaaa ataccggtca tcagttcgat atacgggccg ttattgtcgg tcaggctctt
    51841 gtcccacgcc tggccaaatt cactgtgtcc ccaactccac tgttttttac ctggcgcaat
    51901 atggtggttg gcaacgtgca gcaaaccgcc atcttcatcg tgacaccacg cgccaacaaa
    51961 atcgtactgt gatttttcag ccatatatga ggttggaacg ggcacatttt tatagcgaga
    52021 aatgtccact ccagcggagt agtccacttt gtagtaagtg ccggtggcga tggggaaagc
    52081 ggagacggcc cgtttgccgt gatcaaacac cgccgttaca tccggcggga aaacgctctg
    52141 atgcccttcc ccccctttca ctgccgggtt ggcccaccac aagaaatgac gcggcgtggc
    52201 gttgccgtta tagacgcggc tggcgatttc cagcgccgcc cggtcagggc gcagggtgaa
    52261 acctgtcatc acctgtaaac catgcatcgg ctccgtttcg ccgacccaca ccgtctgtgc
    52321 gccgtcttca tgggcttcga gggtgaaatc aacgggcata aaggtggtcg ggcgatggtg
    52381 ttgcggccag ttaaactcaa tcccaccgga gatccacggt cccagcagcc ccaccagcgc
    52441 aggtttaatg acttcattgt gataaacaaa atcgcgttgt ttcactttat cccatgcgcg
    52501 atgcacacga ccgcccagtt ccggcaggat catcactttg atgtagtcgt tttccagcca
    52561 caccgcctgc caggatttca gggttttctg ctcgctcagc gtatcggtca cgccataggg
    52621 ataaaccgcg ccggacgatc cctgataaac gcgattttcc aggaacatgg gatgtatatc
    52681 ctgcggcccg gtttcatagg tcgggatctc aacgcgctct tgccacactt ttactggagt
    52741 catggtgccc tcaatattaa caagacatac tgaattaaaa gatttgtggc agtgtattga
    52801 acaatctggc aatgttttcg cggaataatc acgcaattaa ctaaacaagg tttagtgaag
    52861 atgagagcct gcattaacaa tcaacagatc cgccaccata acaaatgcgt gattctggaa
    52921 ctgctgtacc ggcaaaagcg cgccaataaa tcaacgctgg cccgactggc gcaaatttcg
    52981 attccggcag tcagtaatat tttgcaggaa ctggaaagcg aaaaacgggt ggtgaatatc
    53041 gacgatgaaa gccatacgcg cgggcatagt agcggtacat ggctgattgc gccggaaggt
    53101 gactggacgc tgtgcctgaa cgtgacgccc accagtattg agtgtcaggt tgctaatgct
    53161 tgtttaagtc cgaaaggtga atttgagtat ttacagattg atgcaccgac gccgcaggcg
    53221 ctgctgtccg aaatcgaaaa atgctggcat cgccaccgta aattgtggcc ggaccatacc
    53281 atcaacctgg cgttggcaat ccacggtcag gttgatcctg tgaccggcgt gtcgcaaacc
    53341 atgccgcaag cgccgtggac aacgccggtg gaggtgaaat atctgctgga agagaagctc
    53401 ggcattcggg tgatggtcga taatgactgc gtgatgctgg cgctggcgga gaaatggcaa
    53461 aataattcgc aggaacggga tttctgcgtg atcaacgttg attacggcat tggctcgtcg
    53521 ttcgtgatta acgagcaaat ttatcgcggc agtttgtatg gtagcggaca gattggtcac
    53581 accatcgtta atccggatgg cgtcgtctgc gactgtggac gttatggctg cctggaaacc
    53641 gtcgcctcgt taagcgcatt aaaaaaacag gcgcgggtat ggctaaaatc acaaccggtt
    53701 agtactcaac ttgatcctga aaaactgact acagcgcagt taatcgctgc ctggcaaagt
    53761 ggagaaccgt ggatcaccag ctgggttgat cgctctgcca atgccattgg tttgagtctg
    53821 tataacttcc tcaacatcct caatattaat cagatttggt tgtacggtcg cagttgtgcc
    53881 tttggtgaga actggcttaa tactattatt cgccagacag gatttaaccc gttcgaccgc
    53941 gacgaaggac cgagcgtgaa agcgacgcaa attggctttg ggcaattaag ccgcgcacaa
    54001 caggtgctgg gaattggcta tttgtatgtt gaggcgcagt tacgacagat ttgatggcgc
    54061 gataacgtag aaaggcttcc cgaaggaagc cttgatgatc ataaacgaaa aattgcctga
    54121 tgcgctacgc ttatcaggcc tacacggaga ttgcaatata ttgaatttgc aagattttgt
    54181 aggcctgaga aggtgttcat gccgcatcca acatgaacaa cgagcacatt gtcagcaaat
    54241 caccgtttcg cttatgcgta aaccgggtaa cgtgcgcaga tgtcgagaac tttacctttg
    54301 atgcgctcga taacggcttc atcattgatg ctgtccagca cgtcacacat ccagccagcc
    54361 agttctttcg cttcggcttc tttgaagccg cgacgcgtaa ttgccggagt acccacgcga
    54421 ataccggagg tcacaaacgg gctcttcgga tcgttcggta cgctgttttt gttgacggtg
    54481 atgttagcac ggcccagagc ggcgtctgct tctttaccgg tcaggttttt atcaaccaga
    54541 tcaaccagga acaggtggtt atcagtgccg ccggaaacca ctttgtagcc gcgctcgagg
    54601 aacacttcta ccatcgcttt agcgtttttc gcgacctgct gctggtaagt tttgaactca
    54661 ggctccatcg cttctttcag agcaaccgct ttaccggcga ttacgtgcat caacggaccg
    54721 ccctgaccac cagggaaaac ggcagagttc agttttttgt acagctcttc gctaccacct
    54781 ttcgccagga tcaggccgcc gcgcggaccc gccagggttt tatgagtggt ggtagtaaca
    54841 acgtgagcat gaggaaccgg gttcgggtag acgccagcag caaccaggcc cgcaacgtgc
    54901 gccatatcaa cgaacaggta agcaccgatg ctgtcagcga tttcacgcat tttcgcccag
    54961 tcaaccacgc cggaatatgc agagaagcca ccgataatca ttttcggctt gtgttctttg
    55021 gcttgttttt ccagatcggc gtagtcgata tgaccggtag catcgatacc gtaaggaacg
    55081 atgttgtaca gtttaccgga gaagttaacc ggagaaccgt gagtcaggtg accgccatgc
    55141 gccaggttca tacccagaac ggtatcacct ggttccagca gcgcggtgta gaccgcaaag
    55201 ttagcctggg agccggagtg cggctggacg ttagcgtagt cagcgccgaa cagttctttc
    55261 gcacgatcga tcgccagttg ttcaacgata tcaacatact cgcaaccgcc gtagtagcgt
    55321 ttgcccggat aaccttcagc atatttgttg gtcagctgag aaccctgcgc ctgcattacg
    55381 cgcgggctgg tgtagttttc ggaggcgatc agttcgatgt gctcttcctg acgtactttt
    55441 tcctgctcca tagcctgcca cagttcggca tcataatcgg caatgttcat ttcacgcttt
    55501 aacatccgca tctcctgact cagctaacaa taaaattttt ggcctttata ggcggtcctg
    55561 ttggacaacg gcgaacagta taaccgaatc attgtgcgat aacaggtctt gacaaaggaa
    55621 tttacgcaaa cgattacctt caggctacgc aaggctttgg agaataaaga gcttgcaacc
    55681 ggaaacggat ttcttttcag gtttgtgatg caaatttttc acttcatcac attctttctg
    55741 aaaaacacca aagaaccatt tacattgcag ggctattttt tataagatgc atttgagata
    55801 catcaattaa gatgcaaaaa aaggaagacc atatgcttga cgctcaaacc atcgctacag
    55861 taaaagccac catcccttta ctggtggaaa cggggccaaa gttaaccgcc catttctacg
    55921 accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt aaccagcgta
    55981 atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt aatattgaaa
    56041 acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc agtttccaga
    56101 tcaaaccgga acagtacaac atcgtcggcg aacacctgtt ggcaacgctg gacgaaatgt
    56161 tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta ctggctaatg
    56221 tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc ggtggttggg
    56281 aaggtactcg cgatttccgc attgtggcta aaacaccgcg cagcgcgctt atcaccagct
    56341 tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg caatatctcg
    56401 gcgtctggct gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac tctttgactc
    56461 gcaaaccgga tggcaaaggc tatcgtattg cggtgaaacg cgaagagggt gggcaggtat
    56521 ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc gctccggcag
    56581 gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct gccggtgttg
    56641 gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac acagcacaag
    56701 tgaactggtt ccatgcggca gaaaatggcg atgttcacgc ctttgccgat gaagttaagg
    56761 aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag ccgagcgaag
    56821 ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc aaactggaag
    56881 gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc ttcatgcagt
    56941 ttaccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat tacgaatgct
    57001 ttggcccgca taaggtgctg taatttgatg ttgccggatg gaaacatccg gcaacccttg
    57061 acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg acccgtgcca
    57121 cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg cgaataatgg
    57181 tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc actttcggca
    57241 gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc tggcgaccaa
    57301 agcctttcac ttcggtcacc gtcatgccgg taataccgac ttcggccagt gcttcgcgga
    57361 catcgtccag cttgaagggt tttataatcg catcaatctt tttcatgcta ttccttgaaa
    57421 aggtcgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat tccttgaaat
    57481 cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg ttgcgccccg
    57541 ccattctcgc cgcgtgggtg acgttgcctt tggtgatttg cagcagctta cgcaaatagt
    57601 tgagttcaaa ctgattacgt gcctcaacaa aggttggcag cgccgtattt tcaccctcca
    57661 gcgcctgctc caccagcgca tcactaatca ccggagatga ggtcagcgcc acgcactgtt
    57721 caatcacgtt gaccaactgg cgcacattac ccggccagct cgcggtcatc aggcgtttca
    57781 tcgcatcggt agagaacgcg cggacaaacg gtttatgtcg ctctgccgcc tggcgcaaca
    57841 ggtgatttgc cagtagcgga atgtcttctg tgcgctccgc cagtgccgga attttcaggc
    57901 tgacaacgtt gaggcggtaa tagagatctt cgcggaattc cccgcgcgcc atcgcttttg
    57961 gcagatcacg gtgagtggca gaaataatcc gcacattgat atcaatatcg cggttactgc
    58021 ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag tttgacctgt aacggcgcag
    58081 gcatatcgcc aatttcatcg agaaatagcg taccgccttc cgccgcctgg aataaacctt
    58141 cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc tccgactcca
    58201 gcaattgctc gggcaatgcg ccacagttaa tagcaataaa tggtttgcta ttgcgcgggc
    58261 tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctgg ccattaatca
    58321 aaacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc agcatcagcg
    58381 ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt ggctggcgcg gattgctcca
    58441 gcgcatcgtc aattgcctga tatagcgcat ctttatcgac aggcttggtg aggaaactaa
    58501 aaacgccctg ctgtgttgca gcaacggcat cgggaataga accatgcgcg gtaagaataa
    58561 ttaccggcat tcccggctgc actttctgga tttcagcaaa cagctgcata ccgtccattt
    58621 catccatccg caggtcgctg ataactaaat ctactttttc gcgattcagt acccgtaatc
    58681 cttcagcgcc actttccgcc gtgaccacac tgtagccttc gctggtcagg cgcaggccaa
    58741 gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt ttatggctca
    58801 tcaggagtga cctcatgggt ggatggcgcg ggcttttcac tctcgtgcgg cgtatccggc
    58861 gagaaatttc cagccggttt gcgggtcgag agctggcgtt caatatcggt caggttttcc
    58921 agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg gcgcaatgta
    58981 tccagctcgc tgtcgctgga ctgctggagt ttactgtaac gttggcgctc ttccgccagt
    59041 tgcagttgca gcgcctgacc gtcgcgccag agttgataca gtgggcgaac ctgtgccggg
    59101 atctcggtac ttaacgcttc aatacgcgcg accagctggc ggcgctcata cggcgtaatt
    59161 ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc gtcgtcatat
    59221 tggcgagctt gctgacgcga ctgcgcaggc attaaacgat cagcacaatc catcgcccgc
    59281 agccagtaaa gcggattggt ttcggttgat ttaccttgca gcgcccagat gtcgctacat
    59341 tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc tggcgtatca
    59401 atggctggct tattgtgatt ctgcacgcaa cccagcaatg ccagacatgg cagccctgcc
    59461 agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac cagacttaaa
    59521 gcctatccca gtgggcgtaa ttgttgcaga cagtctggac atggacagcg cggagaaacc
    59581 ggagcgtaca tatcgtacgt gaagatttcg agcactgccc ggggccgaac tgacaaataa
    59641 aatagcctga taggataggc tgttagcatt atttcgtgtt tttcgacgac ggtaattcaa
    59701 tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct tgcatacggc
    59761 gaatacaatc cctggcaatg cttaatccca gaccgctgcc cttcaccgcc ccttttcgct
    59821 ggtggcttcc ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc ggaatgggcg
    59881 tgcctgtatt gatgacatca atataaaccc gcgcaccatg taaactgctg cgaaggcaaa
    59941 tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc agtacgctca
    60001 tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg gtatgcatca
    60061 ttttagcccg tgcgggcagg ctatgagcag aaaccactgt ctccaccagc ggtgctaatt
    60121 caacattctc cagttccacc gcactgtccg cctgtttacg gttgtaatca agcagttgtt
    60181 cgatcagttt ttgcaaattg cggctgctgc tatcaagaat gctcaccacc tctttttgct
    60241 ctggcgtaag cggcccgaca acctgatcag ccagtaattc agtgccctcg cgcatactcg
    60301 ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgt tgggattcca
    60361 gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt aactcgctcg
    60421 gtccactgaa cgaaacgcta ttgcccagag aacgcccttc ccccagacgg ttgatcatgc
    60481 gctcgatatt tttcaccggc ccgataatca tccgcgtgaa aagtagcacc atcaccagac
    60541 tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg gcgatttcac
    60601 gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt ggcctgcacc atttcggtat
    60661 tggcactggc aaaggcttca agacgcgcgg cggcggcagc atcgggacca ctgttgttac
    60721 actgaagttg ggccagattg ttcaaatcct gacgcaatgc ctggtaaagt ttatcgtctg
    60781 gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg ctttgataaa
    60841 ccttcgcaag cgtagggtcg tccagcacgc aatactgacg gtagctacgc tccatctcca
    60901 gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat aagcgtagtg cggttaacca
    60961 gcgccgcctg atcgctaagc gcattcaggc tttgccaggc ttgccatgcc agcaccaaca
    61021 ggggtagtag aattagcaaa aatgccagca ttaccagttg tcgtaatgag cggggaaaaa
    61081 cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg ctaaccgagg
    61141 ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat aaggcggtgc
    61201 ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa gttggacggc
    61261 aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg gctgacataa
    61321 gtcggtgaat gagccactgc ttactattat gcagtaactg tgccaataaa gaaaatagtt
    61381 tggtaacgta ctgattatac gttgctttga gggatttatg gcttcttcgc tatttgaatg
    61441 taaatcagcc actgtgtcgc taaaaagaga caacttaaga taaacttatt agattacaat
    61501 aaaatcaatc atttaagtgt cgccagaaag cgacacggta aaccacccat tgtcgtgatt
    61561 tacagacaca aaaaagctcc cggagttggg agcttatgat agtggttggt gcttaatgcc
    61621 ggatgcggcg taaatgccgc atccgggctg caaaaccaat gaactgatga cttaccccaa
    61681 ctgcttacgc gcattgcgga aaatgcgcat ccatgggcca tcctcgcccc agttttccgg
    61741 atgccaggag ttgctgacag tacggaaaac acgttccggg tgcggcatca taatggtgac
    61801 tcgaccactt tcagtcgtaa ccgacgtaat accgttcggt gaaccgttcg ggttagccgg
    61861 gtaggtttca gtgactttgc cgaagttatc aacatagcgc agtgccacca gccctttgct
    61921 ttccagtgcc gccagatgcg ccgcatcacg cacttccacg cgcccttcac catgagagac
    61981 agcaatcggc atttgcgagc ccaccatccc ctgcaacagc agagacgggc tttgggttac
    62041 ttcaaccagg ctgaaacgcg cttcaaagcg atcggaggta ttgcgcacaa aacgtggcca
    62101 caactcacta cctgggatca gttcgcgcag gttagacatc atctggcaac cgttacatac
    62161 ccccagcgcc agcgtttgcg gacggtggaa gaaggttgca aactcatcgc gtacacggtc
    62221 attaaacagg attgacttcg cccaaccttc accggcaccc agcacatcac cgtaggagaa
    62281 accaccgcac gcgaccaggg cgtggaaatc ttccaggccc gtgcgtccgg tcagcaggtc
    62341 actcatatgc acgtcgatag catcaaagcc tgcacggtgg aaagctgccg ccatttcaac
    62401 atgcgagttc acgccctgct cacgcagtac ggcaactttc ggacgtgcgc cagtggcaat
    62461 atacggtgct gccacatctt cgttgatatc gaacgacagt tttacattca ggccaggatc
    62521 ggcatcgtta gatttcgcct ggtgctcctg gtcagcacac tccgggttgt cacgcaggcg
    62581 ctgcatctgc caggtagttt ctgcccacca gacacgcaac gtggtgcggc tttcgctgaa
    62641 tacagtctgc ccgttggcgg taatcacaaa acggtcaccg gaaaccgcct gccctacata
    62701 atggacacaa tcagcaagcc catgctgtgc cagtacggac tcaaccgctt cacggtcagc
    62761 ggcacgaacc tgaatcaccg cacccagttc ttcgttaaac aacgccgcca ggcgatcgtc
    62821 gcccagagag gcaatatccg cgttaatgcc gcaatgacca gcaaaggcca tttccgccag
    62881 cgttaccagc aggccgccat cagagcggtc gtggtatgcg agcagcttac gctgtgcaac
    62941 cagcgcctga atcgcgtcat agaagccttt cagttgcgcg acgtcgcgta catctgccgg
    63001 tttgtcgcca agctgacgat aaacctgcgc cagcgccgtt gcgcccagcg cgttattgcc
    63061 tttgcccaaa tcaatcagca gcagtgcgtt atcttcggta gaaagctgtg gcgtgatggt
    63121 gtgacgcaca tcttccacgc gggcaaatgc agaaatcacc agcgacagcg gcgacgtcat
    63181 ttcgcgctct tcgttacctt cctgccagcg ggttttcatc gacatagagt ctttacccac
    63241 cgggatcgtc aggcccagcg ccggacaaag ctcttcgccc acggctttaa cggcttcata
    63301 caggcccgca tcttcaccag ggtggcctgc cgccgccatc cagttggcgg aaagtttaat
    63361 gcgtttgata tcaccaatct gtgttgcggc aatattggtt aacgcttcac cgaccgccag
    63421 acgggcagag gcggcgaaat ccagcagcgc aaccggcgca cgctcgccaa tcgccatcgc
    63481 ttcaccgtag tagctgtcga ggctggcggt agtgaccgcg cagttagcga ccggcacctg
    63541 ccacggcccc accatctgat cgcgcgctac catgccggtt acgctgcggt cgccaatggt
    63601 caccaggaag gttttttccg ccacagtcgg cagatgcagc acacgtttca ccgcatctgc
    63661 aatggtgatc ccttcacggg ccagcgcgtc gcctttcgct ttcagcgttt gtacatcgcg
    63721 ggtcatcttc ggcgttttac caagcaggac gtccagcggc agatcgatcg gctgattatc
    63781 aaaatgacga tcgtgcagag aaagatgcag ttcttcggtc gcttcaccaa tcaccgcgta
    63841 gggtgcgcgt tcacgcttac acagttcgtc aaacagcggt aattgatcgg cagcaaccgc
    63901 cagcacgtag cgttcctggg attcgttaca ccagatttcc agcgggctca tgcccggttc
    63961 gtcgcttaga atatcgcgca gttcaaattt accgccgcgt ccgccgtcgc tcaccagctc
    64021 cggcatcgcg ttagaaagac cgcccgcacc gacgtcgtgg ataaacagaa ttgggttagc
    64081 atcaccaagc tgccagcaac ggtcgatcac ttcctggcaa cgacgctcca tctccgggtt
    64141 gtcgcgctgt acggaagcaa agtcgaggtc ggcatcagac tgacctgacg ccatggaaga
    64201 cgccgctccg ccgccaagac cgatgttcat tgccgggccg ccgagaacga ccagcttcgc
    64261 accgacgttg atctcgcctt tttgtacgtg atcggcgcga atgttgccga tcccgcccgc
    64321 cagcatgatc ggtttgtgat aaccgcgcag ctcttcgccg ttgtggctgt tcactttttc
    64381 ttcataagta cggaagtagc cattcagtgc cggacgacca aattcgttgt taaacgccgc
    64441 gccgcccagc gggccttcgg tcatgatgtc cagcgcggtg acaatgcgct caggcttacc
    64501 gaaatcttct tcccacggct gttcgaagcc aggaattcgc aggttggaaa cggagaaacc
    64561 aaccaaaccc gctttcggct tcgcgccgcg cccggtggca ccttcatcgc ggatttcacc
    64621 gccggaaccg gtcgccgcgc ccggccacgg agaaatcgcc gtcgggtggt tgtgagtttc
    64681 gactttcatc agaatatgcg ccggttcctg atggaaatcg tagcggcccg tttcgtggtc
    64741 agcaaagtag cggcccactt cagaaccttc cattacggcg gcgttatctt tataagcaga
    64801 gagaacgtaa tctggcgtgg tttcgaaggt gtttttgatc atcttaaaca gcgatttcgg
    64861 ctgttgctca ccgtcgataa cccaatcggc gttaaaaatc ttgtggcgac agtgctcgga
    64921 gttcgcctgg gcgaacatat acagctcgat gtcgttcggg ttacgaccaa gctttgtgaa
    64981 agcatcctgc agatagtcaa tttcatcttc cgccagtgcc aggccaagac gcaggttagc
    65041 gtcgattagc gcctgacggc cctgccccag caaatcaacg cttgtgaccg gagtcggttg
    65101 atggtgggca aacaactgct ctgcatcatc taaagcaaaa aagaccgttt ccatcatgcg
    65161 gtcgtgcagt tcagcggtaa cctgctgcca ttgttcattg gtcagcgtac cggcttctat
    65221 atagtaagca acgccgcgct caaggcggtt tacctgttgt agcccgcagt tatgggcaat
    65281 atcggtcgct ttcgaagacc agggagagat ggtgccagga cgcggggtca ccagcaggag
    65341 tttgccttgc ggggcgtggc tggcgagtgc cggaccatat ttcagcaggc gttcaagttg
    65401 tgcgtgctca tcatcgttta acggcgcatt gaggtcagca aaatggacat actcggcgta
    65461 aatattgtga accgggagcc tggcagcctg aaaacgtgcc agcagtttgt tgattcggaa
    65521 tgccgacagt gcaggcgaac cacgcagaat ttccatcata agtctctcgt cttctaagct
    65581 ttcggtgtac ccaagggggg gaaacgggcg tcattataaa gaatctgatg cgctgacgaa
    65641 accgtttgcg tggaaataaa atcaccatcg tgaattagca acgcgtgccg ccaatggatg
    65701 taataagttg ccatctggcg caggtttacg caaaatgccg ctcatttatg agtaaacctt
    65761 tcactattat tacgtttttt caagctggga cgcgcacgac acagagaatt aactaattga
    65821 aaaaattaaa gattaattat ctgttcatcg gcattctggc actgctgctc gcggtcgctc
    65881 tctggccatc cattccctgg tttggtaaag ccgacaaccg tatcgccgcc attcaagcgc
    65941 ggggagagtt gcgtgtgagc accattcata ctcccctgac ttataacgaa atcaacggga
    66001 aaccttttgg cctggattac gaactggcga aacagtttgc cgattacctc ggcgtaaaac
    66061 tgaaagtgac cgtgcggcag aatatcagcc agctgtttga cgaccttgat aatggtaacg
    66121 ccgacctgct ggcggcagga cttgtctata acagtgagcg ggtaaaaaat tatcagcctg
    66181 gccctaccta ttattccgtg tcacaacaac tggtttataa agtgggtcag tatcgcccac
    66241 gtacgctggg caacctgacg gcggagcaac tcaccgttgc accgggtcat gtggtggtta
    66301 acgatctcca gaccctgaaa gaaacaaaat tcccggaatt aagctggaag gtagacgaca
    66361 aaaaaggctc tgcggaatta atggaagatg tcatcgaagg aaaactcgat tacaccattg
    66421 ctgattctgt cgccatcagc ctgtttcagc gcgttcaccc ggagctcgcc gtagcgctcg
    66481 atatcaccga tgaacaaccg gtgacttggt ttagcccgtt agatggcgat aatacccttt
    66541 ccgccgccct gctcgacttc ttcaacgaaa tgaatgaaga cggtacgctg gcacgcattg
    66601 aagagaaata cctggggcat ggcgatgatt ttgattacgt cgatacgcgc acatttttac
    66661 gcgccgtcga tgcggtactg ccgcagttaa agcccctgtt tgagaaatac gccgaagaaa
    66721 ttgactggcg tttgctggcc gctattgctt atcaggaatc gcactgggat gcacaggcca
    66781 cttcaccgac gggtgtgcgc ggcatgatga tgttaaccaa aaataccgcg caaagcctcg
    66841 gcattacgga tcgtaccgat gccgaacaga gcatcagcgg tggcgtgcgt tatttgcagg
    66901 atatgatgag taaagtgccg gaaagtgtgc cggagaacga gcggatctgg tttgccctcg
    66961 ctgcgtacaa tatgggctat gcgcatatgc tggatgcccg cgccctgacg gcaaaaacca
    67021 aagggaatcc tgacagttgg gctgacgtaa aacagcgtct gcctttactt agccagaaac
    67081 cctattacag caagctgact tacggctacg ctcgtggaca tgaagcctac gcttatgtcg
    67141 aaaatattcg taagtatcag attagcctgg tgggttatct gcaagagaaa gagaagcagg
    67201 ctacagaagc ggcgatgcaa ctggcgcagg attatccggc ggtatcgcct acggagttgg
    67261 gcaaagagaa atttcctttt ctctcgtttc tttcccagtc gtcatcaaac tatttgaccc
    67321 attctccctc tctgctgttt tccaggaaag ggagtgaaga gaaacaaaat taatccgtcg
    67381 aggattgcgc ttttttctgc gctttaattt cctggcggcg catgcgaaag aagtcactga
    67441 gcaacgccgc gcactcatcc gccagtattc cttccgtaat ttccactcgg tgattcatac
    67501 ccggatgatg cagcacatcc attaaagatc ccgcagcgcc agttttcgcg tcacgcgcac
    67561 caaagaccac gcgaccaatg cgactgtgga tcatcgctcc ggcacacatt acacatggtt
    67621 caagcgtgac atacaatgtg gcgtcgatca gacgataatt ttgcatcacc agaccaccct
    67681 gccgcagggc catgatttct gcatgtgcgg tgggatcatg gcgaccaatc gggcggttcc
    67741 agccttcgcc gattacccga ttgttatgca ctaataccgc gccgaccggc acttcccgct
    67801 catcccaggc acgtttcgcc agcgtcagcg cgtgacgcat ccagtattcg tggctaaatt
    67861 cgacttcaga caaaaagaaa actccggtta taaaagcgcg gcgcattata cacggacgct
    67921 atgctttact ctattccagt tgctggagtt caccgcgcgg ggttactcgc caacgatgct
    67981 gacagaaata aagcagcgga ttgtcctgat tactgtcgct atagccactg tacagccgca
    68041 gcggagtgcc gattttgcgc tccagttgtg cgaccttttc atgtcccaga caacgcatcg
    68101 tcaataccca accaccatag ccacgctgaa tttggctggc gataagatta acccgcggca
    68161 gccagggcgt atcgaaataa accgcttcaa ccagcggctg cggagagccg gtaatcaacc
    68221 agatatcagc atcggaactt aacaggtagg tggttaatcg ctcctgaacc agcggaaagg
    68281 cggtaacatt gtcgcgaaac cagcgcacga aatcggcctg caacgtctgt aaacgtgctt
    68341 cgctgtgacc aaaagtgcac ccccacagaa gcagactcat cggccagcgt gccgcacgac
    68401 cttttatcaa taacgcaatg gctataatcg gtaacaacgg caggacaagt aacgcattca
    68461 gcggttggcg acgtagtaaa tagcgcagaa aactgccgaa catatcctgc tgatgcaatg
    68521 ttccatctaa gtcaaaaaac accacacgac gctcgtgagt tgccaaacca tactcctctg
    68581 gactgaaaca tcctgattaa ttttcgctac tacatagcct aacagataga tcatcacttt
    68641 tccggcaaca atcgaccgca tcatggctaa ctggaatttt taattcatgg caattagcgg
    68701 caatggaata taaaattcac tcgcgtgtgt ctcatattgc ctgttgtcgc cactatttca
    68761 tcgcgaggaa agatatgaac ggcttacttc gtatccgtca gcgttaccag gggcttgccc
    68821 agagtgataa aaaactggcg gattatctgc tgctacaacc tgatacggcg cgccatttaa
    68881 gctctcagca actggccaac gaagccggag tcagtcagtc cagcgtcgtg aagttcgcgc
    68941 aaaaactcgg ctataaaggt tttccggcgc ttaagttggc gttgagtgaa gcgctggcaa
    69001 gccagccgga atcaccctcc gtgcccattc ataaccaaat ccgcggtgat gatccgttac
    69061 ggctggtcgg cgaaaaactg attaaagaaa atactgccgc tatgtacgca acgctaaacg
    69121 ttaatagtga agagaaactg catgaatgcg taacaatgtt gcgctctgcg cggcggataa
    69181 ttctgaccgg tattggcgct tcgggtctgg tggcgcaaaa ctttgcctgg aagctgatga
    69241 agattggctt caatgctgcc gcagtacgcg atatgcatgc gctactcgca acagtacagg
    69301 cgtcgtcccc tgacgatctg ttattagcca tttcctacac cggtgtacga cgcgagttaa
    69361 acctggcggc agatgagatg ctgcgagtgg gcggaaaagt gctggcgatt accggcttta
    69421 ctccgaatgc cctgcaacag cgtgcttctc attgcctgta taccattgcc gaagaacaag
    69481 cgacaaacag tgcttcaatc tctgcttgtc acgctcaggg aatgttaacg gatttgctgt
    69541 tcattgcgct gattcagcag gatctggaac tggcaccaga acgtattcgt catagtgaag
    69601 cgctggtgaa aaaactggtc tgagtaaaga atgcgcgtat aatgcccgcc cggtttgtgt
    69661 tgttttgaga gtttccttat ggcgttgtta atcactaaaa aatgcatcaa ttgtgatatg
    69721 tgtgaacccg aatgcccgaa tgaggcgatt tcaatgggag atcatatcta cgagattaac
    69781 agcgataagt gtaccgaatg cgtagggcac tacgagacac caacctgcca gaaggtgtgc
    69841 ccgatcccca atactattgt gaaagatccg gcgcatgtcg agacagaaga acagttgtgg
    69901 gataaatttg tgctgatgca ccacgcggat aaaatttaat attctactct ggaagtagag
    69961 tattaattat attactggga agccttaacg ccattatatt tatttaattg atgacattag
    70021 cataatcatt cactaagtta atttatatag tatctgccca gacacttatt tatagttatt
    70081 aaaggtgcgt ccgactggtt caccggacgc accttaagta cgtttccttg tgttataaga
    70141 acagaaggat cagctgtaaa acagcaatga tgattttgat gacccgttta atcaggtatc
    70201 ggcaatcagt cattcgtttt tccttaaaca aggaatgcag ccattcagat tagcccttac
    70261 atctccccaa aactgaacgt gcgagttatt gagggtgcat gctgcactcc acaccagagc
    70321 tttgacgaca ccactcgttt caatggggga attctgtggc atggtgtaaa gcacagcaaa
    70381 atcttcaata acgaagccaa ttttaatgta cttacgaatt ggcggtcacg ttaatctttc
    70441 catcaatatt gctttcttcg taaaggctcg agtttttatg ctaaagattg caagttgctt
    70501 gtaaaagata agtacactga tccataatcg ctgttgttga gggtgcatgc tgcacaaaat
    70561 taaagttaaa aagtaaaacc cccgttcctt accagttcgg gggttttact ttttaaagag
    70621 aacggtatta tttttaactt tcaataatta ccgtggcaca agcatagtgc cgctcatctg
    70681 ccagcgttac atgcatattt gcaacgccca gcttttccgc cagttttaat gcctcgcccc
    70741 atagccgtag ccgtggtttg ccgagctcat cattgaatac ttcaaattga ttaaacgcca
    70801 gaccattgcg gatcccggtg ccaaacgctt ttgctgcggc ttctttcaca gcaaaacgct
    70861 tcgccagaaa acgcaccggc tggtggtgcg ttttccagat agcccattcg ttatcgctta
    70921 atacgcggcg tgccaggcga tcaccggatc gggcgatcac cgcttcgatg cgagcgatct
    70981 ccacaatatc cgtgcctaaa cctaatattg ccattagcca cgcgcttcca gcatcagacg
    71041 cttcatttct gccaccgcat ctttcagtcc ggtcatcact gcacgaccaa taatggcatg
    71101 accgatattc agttcatgca tctcagggat ggcggcaatg gctttcacgt tgtgataggt
    71161 cagaccgtgt ccggcgttaa ctttcagacc gaggcttgcg gcaaaggtcg cggctttggc
    71221 gatacgcgcc agctcttgcg cctgttcggc gtcagttttg gcatcagcat agcaaccggt
    71281 gtggatctcg ataaacggtg cgccaacctc tgccgcagct ttgatctgct cttcatcggc
    71341 gtcaataaac agagaaacct gaatcccggc atctgccaga cgtttgcagg catcgcgcat
    71401 tttgtcacgc tgccctgcga catccaggcc gccttcggtt gttacttcct gacgcttttc
    71461 cggtaccagg cagcaaaaat gtggcttcgt ctcaacggcg atcgccagca tctcttcggt
    71521 caccgccatc tccagattca tgcgggtatc cagcgtctga cgcaggatgc gcacgtcgcg
    71581 gtcagtaatg tgacggcgat cttcacgtaa atgcacggta atgccgtccg ctcccgcctg
    71641 ctcggcaata aacgcggcct gcaccggatc cgggtaagcg gtaccgcgcg cgttgcgcag
    71701 cgtagcgata tggtcaatgt tgacgcctaa cagtaattca gccatgacaa tcctcatcat
    71761 tcataatgtg ttttcaccgt tcgcttaggc ataaattgcc ggaacagttc cctgctcttt
    71821 aaaggtttac cgccaagata cggcttaagc gccatgcggg taaagcgttt cgcggcgcgc
    71881 agtgtgtctg cgtcaggaaa ttcccgtgcg tttaacgctt ttaactgcct tccggtgaac
    71941 gttttattgt cgataacgac gcttgcgata aacccttttt cttcgcgata acgatacgtc
    72001 atggtgtcat ctaccggctc gccgctaccc gcacaatggg taaaattgac gccataaccc
    72061 agatgcccga gcagtgccag ttcaaagcgg cgcagcgcgg gttctggcgt accagtgacc
    72121 cctgcaagag actgaatgca gtgcaagtaa tcgaaaaaaa gttcagagaa gcgcgtctcg
    72181 tattccagta cgcgggagag aagttcgttg atgtacagac cgctgtaaag cgtgataccg
    72241 cttaatggca gcgccagcga gacggcttca gcactgcgca gcgttttgac ttcgccacgc
    72301 ccgccaaaac gtagcaagag aggggtgaaa ggctgtaatg cacctttcag ggtagagcgt
    72361 ttagagcgtg cgcctttggc aaccagacgc acgcgccccg attcctccgt gaagacgtcc
    72421 agcatcaggc tggtttcgct ccacgggcga ctatgcagga caaatgcgcg ctgccagcct
    72481 tccatcggag ttactcttaa agatcgtcaa cgtaaccgag actgcgcagt gcgcgttcgt
    72541 cgtcggccca accggatttc acttttaccc acagttcaag gtgaacaggc gcttcgaaca
    72601 tttcctgcat gtctttacgc gcttcaatcc cgatggtttt gatcttggcc cctttgttgc
    72661 caatgaccat cttcttctgc ccttcacgct caacgagaat caaaccgttg atgtcataac
    72721 caccgcgttc gttagagacg aaacgttcga tctccacggt cacggagtac ggcagttcag
    72781 cgccgaggaa acgcatcagt ttttcgcgga tgatttcaga cgccataaaa cgctgtgagc
    72841 gatcggtgat gtaatcttcc gggaagtgat gagtcgcttc aggtagatgc ttacgcacga
    72901 ttgccgcaat agtgtcaaca ttcagcccgg tttcggcaga gattggcacg atatcgagga
    72961 agttcatctg gcttgccagg aactgcaggt gcggcagcag atcggctttc tcctgcacgt
    73021 tgtccacttt gttcaccgcg aggattaccg gcgctttgcc ttcgcgcagt ttgttgagca
    73081 ccatttcgtc gtccggcgtc cagcgggtgc cttcaacgac aaaaatcacc agctcaacat
    73141 cgccaataga gctgctcgcc gctttgttca tcaggcggtt aatggcgcgt ttttcttcca
    73201 tatgcaggcc cggtgtatcg acgtagatcg cctgatacgc gccttcagta tggatcccca
    73261 caatgcggtg acgagttgtc tgcgccttgc gggaagtgat ggagattttc tgccccagca
    73321 gtttgttcaa caatgtggat ttgccaacgt tcggacgtcc gacgatggca ataaatccgc
    73381 agtaactttt atcgatgctc attccagctc cagttttttc aacgcctgtt cggcggcagc
    73441 ctgctcagcc ttacgacggc ttgaacctgt gccaaccacc ggttcactca ggccgctgac
    73501 ctggcagtgg atagtaaatt cctgatcgtg cgcttcgcca cgtacctgga ctaccagata
    73561 agtcggcagc ggcagatggc gaccctgcaa atattcttgc aagcgcgttt tcggatcttt
    73621 ttgtttatcg cctgggctaa tttcgtccaa acgagtttga taccagttga ggattaattt
    73681 ctcgacggtt tgaatatcac tgtcgaggaa tacgccacca attaatgctt cgacggtgtc
    73741 ggcgagaatt gactcacgac gaaatccacc gcttttaagt tcacctggcc ctaaacgtaa
    73801 gcactcgcct aactcaaatt cgcgcgccag ttccgccagc gtattgccac ggaccagcgt
    73861 ggcgcgcatc cggctcatat cgccttcatc cacacgaggg aaacggtgat aaagcgcatt
    73921 ggcgataacg tagctcagaa tagagtcgcc taaaaattct aaacgctcgt tatgtttact
    73981 gctggcacta cgatgagtta atgcctgctg caacagttcc tgatgattaa aagtgtagcc
    74041 cagcttccgt tgaagccgat taattacgat ggggttcatg cgataccaat aaataaatgc
    74101 gtcaacaatt cagcacacga aacagacctg atatacatgg ttctgctaac tgcttcgctg
    74161 cagtttcagt ttaccggtat atggggacca acgctgtttc gtgtgccgtg gcaacctgga
    74221 ggtgccaacc ttaaacttcg ggggaatatt ctatacacaa cgacggggga tgtcgttagc
    74281 cacgggagat ttatctcata aataattcac gttgtcgcca taacggcgac aacgtgaacg
    74341 aagatggcta ttaatggatg ccgccaatgc gacttaagcg cagaccagtc ggccattcgc
    74401 cttcttgctt atcgaagctc atccagatag ccgttgcccg accgaccaga ttcgcttccg
    74461 gcacaaagcc ccagtaacgg ctgtccgcgc tgttgtcgcg gttgtcgccc atcatgaagt
    74521 attgtcccgg aggaacaatc caggttgcca gttgttgccc tggctgctgg taatacatcc
    74581 ccacctgatc ctgcgcaatc ggcactgtca gaatgcggtg cgtcacatca cccagtgtct
    74641 ctttacgctc ggaaagacga attccatttt ctttggtttc gtttttcggc acttcaaaga
    74701 atccgctggt cgcttcccca ccattacggc gtgagaaggt ctgaacgaaa tcgctcggtt
    74761 ccacgtttga gtaggtgacc ggcagcgcgt tttcacacgc ctggccggaa ctgcatcccg
    74821 gttgaatcgt cagctctttt gagaccggat cgtaagtgac tttatcgccc ggtaaaccca
    74881 ccgcgcgctt gatgtaatca agctttggat cttccggata tttaaagacc acgatatcgc
    74941 cgcgtttcgg atgaccggtt tcgatcagcg ttttctggta gataggatct ttaatgccat
    75001 aagcaaactt ctctaccaga ataaaatcac caattaacag agtcggcatc atcgaacctg
    75061 acgggatctg gaacggttca taaataaacg aacgcacaat caatacgata gccagtaccg
    75121 gaaaaacaga agcaccggtt tccagccagc caggcttcgg cgcaaccttt ttcaacgttg
    75181 ctttatccag tgagtccccg gcagccgcct gcgccgctgc ctgacgttcc cgccgtttag
    75241 gtgcgaaaaa gaatttatcc acgcaccata aaatgcccgt caccagtgtg gcaatcacca
    75301 gaatcagggc aaacatattc gccatgccaa ctcctaaggg ttatttgttg tctttgccga
    75361 cgtgcagaat ggcgaggaac gcttcctgcg gcagctcgac gttaccgatc tgcttcatgc
    75421 gtttcttacc ttctttctgc ttctgcagca gctttttctt acggctgata tcgccgccat
    75481 aacatttagc cagtacgttt ttacgcagct gtttcacggt ggatcgcgca atgatgtgcg
    75541 taccaatcgc tgcctgaatg gcgatatcaa actgctggcg tgggatcaga tctttcatct
    75601 tctccaccaa ctcgcgaccg cggttttgcg aattatcacg gtgggtgatc aacgccagcg
    75661 catcaacacg ttcaccgttg attaatacgt ctacacgtac catgtcggac gcctggaagc
    75721 gcttgaagtt gtaatccaga gacgcataac cacgcgaggt agatttcagg cgatcgaaga
    75781 agtcgagcac cacttccgcc atcgggatct cgtacgtcag cgccacctga ttaccgtggt
    75841 aaaccatatt ggtctgcacg ccgcgttttt ctacgcacaa cgtaataacg ttgccgagat
    75901 atgcctgcgg cagcagcatg tgacactctg caatcggctc gcgcagttcg tagatgttat
    75961 ttaccgcagg cagcttggat gggctgtcga cgtagataac ttctcttgac gtggtttcaa
    76021 cttcatacac tacggtcggc gcagtggtga tcagatccag atcgtattca cgttccagac
    76081 gttcctggat gatctccatg tgcagcaggc cgaggaagcc gcagcggaaa ccaaagccca
    76141 gcgcgctgga gctttccggc tcatagaaca gtgaggcatc gttcaggctg agtttaccca
    76201 gcgcgtcacg gaaggcttca tagtcgtcgg aacttaccgg gaacagaccg gcgtatacct
    76261 gcggtttgac tttcttaaag ccaggcagcg ccttttctgc cggattacgc gccagcgtta
    76321 aggtatcgcc gactggagcg ccgtggatat ctttaatcgc acatacgagc cagcctactt
    76381 cgccacattt cagttcagtg cggtcaacct gtttcggcgt gaagatgccc agacggtcgg
    76441 cgttataggt ctgcccggta ctcatgactt tcactttgtc gcccttacgc agggtgccgt
    76501 ttttaatacg gataagtgaa acaacgccca ggtagttgtc gaaccatgag tcgataatta
    76561 gtgcctgcaa cgggccttcc ggatcgcctt ccggcggcgg aatgtcgcgc accagacgtt
    76621 cgagaacgtc ctgcacacca acgccggttt tcgctgaaca gcgcaccgcg tcggtggcgt
    76681 cgatgccgac gatatcttca atttcttccg ccacgcgttc aggatcggct gccggcaggt
    76741 caatcttgtt cagtaccggc acaacttcga gatccatttc catggcggtg tagcagtttg
    76801 ccagggtttg cgcttctacg ccctgcccgg cgtcgaccac cagcaatgca ccttcacagg
    76861 cagccagcga acgggaaact tcataggaga agtctacgtg gcccggggtg tcgataaagt
    76921 taagctgata ggtttcgccg tcagacgctt tgtagtccag cgtcacgctt tgcgctttga
    76981 tggtaatgcc acgctcacgc tcaagatcca tggaatcgag aacctgcgcc tccatttcac
    77041 ggtcagacag gccaccgcag atctggataa tacggtcaga cagcgtcgat ttaccgtggt
    77101 caatgtgagc tatgatcgaa aagttacgta tattcttcat aaagtatgat tattgtgcct
    77161 taatgcccgg ttaaccaggc ttttagaagt cgctgttctg agcttaacgt ctgtattaat
    77221 agaaacgccg cattctacac tacaacattg aggcgaggaa atgttcatac cgtatggatt
    77281 gtggtatctg gaaacgtcct cgcatttgtt atgcaaaatg caacaaagcc agtgaaatca
    77341 ctggctcgcg tcttccgaag atgtttcaaa tcgcacaagg ccaggcggca atgccacgct
    77401 taagatgatc ggttgccatt ctgcccgggc agcaaactta cgcgagtagc cgcgcgcaat
    77461 caggaacccg ccaataccac cgaggatcgc accacataat gctgcgacgt cagaagcaaa
    77521 gagtagctga aatagcgaag cgataaggaa taatcccacc agcggcgaca tataaaccag
    77581 taatgcggag ctaagcaggc tgccttcggc gatccccaat tccacttttt gccccggcac
    77641 taacggctca tcacagggta cgacaatggt atgcgtggtt tgcgggccaa gtttatttaa
    77701 cacgcggcta ccgcaaccgg cgcgtgaagc gcagctgctg catgaagctt taacatcaca
    77761 actgaccagc gcctgcccgt tttgccagga gacgacggta gcccactctt tgatcattgc
    77821 gctgccccga acttaatatt ctcggcaatg cgtttcgccg tttgcggcgg cagttcaccg
    77881 acaatggtga tttcggcgtt atcacgtacg cttgtactga cggttctgcg tccggtgcgc
    77941 aacatctgat cggtgctcga tggcgtagcg cggttaacgt ttaccgagaa gctgaataat
    78001 ccgtcggaat agagacgtga ttcgataggc atgttgtcca tcgtcggtag cggacgtcga
    78061 ctactggaaa cttcgctaaa accctgtggc aaccaggttg gcgtccagct gaatttagct
    78121 ttttcaccta caggaacaga aagcaacggc ggcaaatttg ccttcgccag cgtctgcata
    78181 ctgctgctga tatcctgatt gacgttaaaa gcaatcacgc gaaattgttc cagcgtttca
    78241 ccatcgcgat caaggagatc aacccgcatc ggtaatttcg attcggtgtc catccacacg
    78301 atgtagctgt agcgtgtacc atctcgggca accacgcgaa tgacttcgca aagacgatca
    78361 gcaatacgcg tgcgcccgac ggagataaag tcgtagtaag gagaaaggcg tttgaaatcg
    78421 gtatagataa gcgatggcag agaatcaaca atgtaatcgc cattaagcgt gaacggttca
    78481 agtcccggtt caaaatagct gatttcattg ccgcgctgta ccacttcccg gcgcgggcca
    78541 tccatttgca acaattgtgc aagaggacgg ttatcgaggc gtgcatgtcg ataacgcaga
    78601 gactcaacac cctgtttatt gatgctgatg aatgacagct cgtaattcag tgactgactg
    78661 gccaggttca tctgctgtaa taacgccccg gacgcgggag tggccgaggc gttagcagag
    78721 aataacaggc tacctgtcac taatgacatg gcaaaccaaa gttgcttcat tactgcgatt
    78781 gcgttcctaa agtttgaatt cctggcacct gtacagcggc ttgctgggtt tgcgcctgct
    78841 caaactgaag ctgttcagag tggagtcggc gttgcagttc gtaatcctgc aacattgcat
    78901 taatgcgacg acgctgctcc tgtacctgct gctgttgacc attgtttgcg gtcgcttcag
    78961 aaggtactcc caggcttacc gggctggctt tacccatcat cggcagtgta ttaaataccg
    79021 gcgtttcggg ctgctgggac gtttcagatt gtccattata gtgctggacg ccaacgataa
    79081 ctgcaagcga tacgcatgcg gctacgccca tttgggtaag ctgtgccgcc cacggacgta
    79141 ctttctgcca gaatggcatt ttctgccatt gatgcggcgc aggctgggct tccgggatca
    79201 atgtcgccgg ttgacgtact ggctcttctt caatggcggc catcacgcgt gaagagatat
    79261 cgaaatggag cacctcggga gtatcacccc gcattgagtc acggattaag tgatagcttt
    79321 cccaggtttt ctgcatttct gggttatgag ccagttcgtt aagcagctca ctatccagcg
    79381 tttcgccatc cattaaagcg gaaagttgtt ctttctgcat gcctaatacc cttatccagt
    79441 atcccgctat cgtcaacgcc tgataagcgg ttgaactttg ttatcaatag cttccctcgc
    79501 tcggaagata cgtgaacgca ccgtacctac cggacaatcc atgatagcgg ctatctcttc
    79561 atagctcagg ccatccagct cccgcaaggt tattgccatg cgtaaatctt ccgggaggga
    79621 ctcaatagtt cggaaaacta tctgtctcag ttcttctgac aacattaagt tctcagggtt
    79681 cgaaatttct ttcaacgcgc cgccactttc gaagttttca gcttcaatgg catccacatc
    79741 actggaaggt ggacgacgcc cctgagcaac caggtaattt ttcgctgtat ttacagcaat
    79801 ccgatacagc catgtataaa aagcgctatc tccccggaac gaatccagcg cacgataggc
    79861 tttaataaaa gcttcttgta ccacatcggg aacatcaccc gacggcacat agcgggaaac
    79921 cagactcgcc actttatgct gatagcgcac taccagtaag ttaaaggctt tctgatctcc
    79981 cttctggacc cgttcaacca ggacctggtc cgttaactgc tcgctcatcc gaggtaaagt
    80041 ctccccaaac caaatttcca cgcgctatcg aaacgccact ccattagctg caatttgagc
    80101 aagcaaaggg ttagagtgtc tcgtttttgt aaagttccgt aacgcatctg tttttgtttg
    80161 tcatgctgta gacggatcat tatctatcat tataagtcta cagaatctga acatcgcatt
    80221 atctgtgtag aaatgcccat ttaactgcct gaagagtaac ccaacggcct ttttatttca
    80281 ccacctaatc ctccaccagc cagtaacttc tctttttctc gccgccctgc gtcagcgtgt
    80341 ttagccactg taacaaatat taaaatagca ggtgtttatt cgcacaacat gatgctatgc
    80401 tgaccaaacc atgtttagta aattaaacaa agaaaatgaa tactctccct gaacattcat
    80461 gtgacgtgtt gattatcggt agcggcgcag ccggactttc actggcgcta cgcctggctg
    80521 accagcatca ggtcatcgtt ctaagtaaag gcccggtaac ggaaggttca acattttatg
    80581 cccagggcgg tattgccgcc gtgtttgatg aaactgacag cattgactcg catgtggaag
    80641 acacattgat tgccggggct ggtatttgcg atcgccatgc agttgaattt gtcgccagca
    80701 atgcacgatc ctgtgtgcaa tggctaatcg accagggggt gttatttgat acccacattc
    80761 agccgaatgg cgaagaaagc taccatctga cccgtgaagg tggacatagt caccgtcgta
    80821 ttcttcatgc cgccgacgca actggtagag aagtagaaac cacgctggtg agcaaggcgc
    80881 tgaaccatcc gaatattcgc gtgctggagc gcagcaacgc ggttgatctg attatttctg
    80941 acaaaattgg cctgccgggc acgcgacggg ttgttggcgc gtgggtatgg aaccgaaata
    81001 aagaaaaggt ggaaacctgc cacgcaaaag cggtggtgct ggcaaccggc ggtgcgtcga
    81061 aggtttatca gtacaccacc aatccggata tttcttctgg cgatggcatt gctatggcgt
    81121 ggcgcgcagg ctgccgggtt gccaatctcg aatttaatca gttccaccct accgcgctgt
    81181 atcaccccca ggcacgcaat ttcctgttaa cggaagcact gcgcggcgaa ggcgcttatc
    81241 tcaagcgccc ggatggcacg cgttttatgc ccgattttga tgtgcgcggc gaactggccc
    81301 cgcgcgatat tgtcgcccgc gccattgacc atgaaatgaa acgcctcggc gcggattgta
    81361 tgttccttga tatcagccat aagcccgctg attttattcg ccagcatttc ccgatgattt
    81421 atgaaaaatt gctcgggctg gggattgatc tcacacaaga accggtaccg attgtgcctg
    81481 ctgcacacta tacctgcggt ggtgtaatgg ttgatgatca tgggcgtacg gacgtcgagg
    81541 gcttgtatgc cattggcgag gtgagttata ccggcttaca cggcgctaac cgcatggcct
    81601 cgaattcatt gctggagtgt ctggtctatg gctggtcggc ggcggaagat atcaccagac
    81661 gtatgcctta tgcccacgac atcagtacgt taccgccgtg ggatgaaagc cgcgttgaga
    81721 accctgacga acgggtagta attcagcata actggcacga gctacgtctg tttatgtggg
    81781 attacgttgg cattgtgcgc acaacgaagc gcctggaacg cgccctgcgg cggataacca
    81841 tgctccaaca agaaatagac gaatattacg cccatttccg cgtctcaaat aatttgctgg
    81901 agctgcgtaa tctggtacag gttgccgagt tgattgttcg ctgtgcgatg atgcgcaagg
    81961 agagtcgtgg cctgcatttt acgctggatt acccggaact gctcacgcat tccggtccat
    82021 cgatcctttc tcccggcaat cattacataa acagataaaa agcctgggtt agcgccgtat
    82081 acgcttcgga atagttctgg tctggcccac gaatgactaa gcgatcgcta aagcattctc
    82141 ccgcctgcgg ggagaatgcc agcagcaccc gatgcggcag tcgcgcttcg ttttccgcca
    82201 catccgtccg caaacgtaaa tgccagccca tgcttaatgc cagctccgta aaaccattac
    82261 caatctgctc tggcagcact acgcagaaaa atccctcttc ggtaatgcac tccgccgcac
    82321 aggtcagcaa cgatgggtga tcaagcgtag tggtatagcg agcctgttcc cgttgaggtg
    82381 tcgagcactc tactccctgc tgatagtaag gtgggttact gatgattaaa tcgaagcgta
    82441 ctgtctgctg tgtgatccac tgctgaatat ccgccgtatg gacgttaatc cgctctgccc
    82501 acggggactg gttgatattt tcctgcgcct gcgcggcagc ttcactttcc agttcaactg
    82561 catcaatcat cacgctgtca tcggttcgct gcgccagcat taatgccagc aacccgctac
    82621 ccgcgccgat atcaaggcaa cgttttaccc cagccaccgg tgcccatgcg cccaataaaa
    82681 taccatccgt tcccactttc atcgcacagc gatcgtgagc aacaaaaaac tgtttaaaag
    82741 taaatccatt acgacgaagc acggatgtag actgtgacat gaaaataaaa ccttgcagga
    82801 aaaacggcga tagcaccggg tgagaacaat acccgagaag cgatatccat acaaacagat
    82861 gaagattgca gccgtaacgt ctataatcag cgccccacac agaggtagaa catgactgta
    82921 acgacttttt ccgaacttga actcgacgaa agcctgctgg aagccctcca ggataaaggt
    82981 ttcactcgcc cgaccgccat tcaggctgcc gccattccgc ctgcgctcga tggccgtgat
    83041 gtactcggtt ctgcgccgac aggcaccggt aaaacggcgg cgtatctgct gccagcgttg
    83101 cagcacctgc tcgatttccc gcgtaagaaa tccggtccgc cgcgtatttt gatcctcacc
    83161 ccaactcgcg agctggcgat gcaggtggcc gatcatgccc gcgaactggc gaaacatacg
    83221 catctggata tcgccaccat caccggcggc gtagcctata tgaaccacgc ggaagtgttc
    83281 agcgaaaatc aggacatcgt ggtcgccacg accggacgtc tgctgcaata cataaaagaa
    83341 gagaacttcg attgccgcgc ggttgaaacg ctgatcctcg acgaagcaga ccgtatgctg
    83401 gatatgggct tcgctcagga tatcgaacat attgctggcg aaacgcgctg gcgtaaacag
    83461 accctgctct tttcggcaac gctggaaggc gatgcgattc aggactttgc cgagcgtctg
    83521 ctggaagatc cggtggaagt ttctgccaat ccctccaccc gtgagcgcaa aaaaattcat
    83581 cagtggtatt accgcgccga tgatcttgag cataaaaccg cgttgctggt gcatctgtta
    83641 aaacagccgg aagcgacccg ctcaattgtg tttgtgcgta agcgtgagcg tgtgcatgag
    83701 ctggcaaact ggctgcgcga agcgggcatc aacaactgct atctcgaagg tgagatggta
    83761 cagggcaagc gtaacgaagc gatcaagcgt ttgaccgaag gtcgcgtaaa cgtactggtc
    83821 gcaaccgatg ttgccgcgcg cggtatcgac attcctgacg tcagccacgt ctttaacttc
    83881 gatatgccgc gcagtggcga tacttatttg caccgtatcg gacgtaccgc gcgcgccggt
    83941 cgtaaaggca ccgcaatttc gctggtggaa gcccatgacc atctgctgct gggtaaagta
    84001 ggccgctata ttgaagagcc aattaaagct cgcgttattg atgagttacg cccgaaaacg
    84061 cgtgcgccaa gcgaaaagca gaccggcaag ccatcgaaga aagtactggc taaacgtgct
    84121 gagaagaaaa aagctaaaga gaaagagaag ccgcgggtga aaaaacgcca tcgcgacacc
    84181 aaaaatattg gtaagcgccg taaaccaagc ggaacgggcg tgccaccgca aacgacagaa
    84241 gagtaatctc aatgccaggt ttaagcctgg tattaaaagt gctggcaaac gcaaaactgc
    84301 ctgatgcgct acgcttatca ggcctacgtg gctcatgcaa tatattgaat ttgcacgatc
    84361 ttgtaggccg gataaggcgt tcacgccgca tccggcatga ataaagcgca ctttgtcgac
    84421 aagttaaatg ccgggtttaa tcccgacatt atttttatcc agccacaaaa ctctcttcaa
    84481 cacactgaat aaaagtgtgc atcgccgggc taaccgcttt tccagcatga tgtgcgcaca
    84541 tggccgtaat ggtctgcgac tgttcgccaa aaggcaattc aattaattct ccgcactcca
    84601 actcttttgc caccgcaaag cgcggtaggt agctgacccc gatattcgcc gcgacacaac
    84661 gcttgatgct ttcgatactt ataagctcaa tggtgttttc caccgtgatc cgccgctgac
    84721 gcagcgtgct ctcaaatatc tgccggaaga cacattgcgg ttcgttgata ataaagctac
    84781 aggcgttatg tcttcccggc tcagtaaaat cgacatctgc aatttgcggt gaagccacca
    84841 gcaccagtga ttgttcaccc aactctcgtc gattcagagc atcatcattc cctacacgat
    84901 aaaagacgcc aacatcggcc tcatcattca gtagcgcatc acggatcacg taacagttca
    84961 gcgactgcaa cgataaacgc acttttgggg cgcgttgccg aaaacgctgc aacacctgcg
    85021 gcattcggta agagagcaac gtttcgcccg aaacaacgcg tagttccccg tccggatctg
    85081 actccttttt ggcagcctca cgaagcgtat ccatcactcg ggtaagttca taaatgtgcg
    85141 gcagcaactt ttttccttcg cgggtaaggc acatccgtcg gccaattttc tcaaataact
    85201 ggactgaaaa ctcctgctca agctgctgaa tatgaaaagt caccgtcgat tgtgtacagc
    85261 acaatttttg cgaagctcgc aaaaaggaac cctcttccac cacggtttta agcgtaataa
    85321 aacggcgcag atccataacc ccaaacctat cgaaaatatc gaatctagaa tataaaaaca
    85381 ttcatttttt taaatgttcc gtgtcgggta ctgtctacca aaacagagga gataacaagt
    85441 gacaccgacc cttttaagtg ctttttggac ttacaccctg attaccgcta tgacgccagg
    85501 accgaacaat attctcgccc ttagctctgc tacgtcgcat ggatttcgtc aaagtacccg
    85561 cgtgctggca gggatgagtc tgggattttt gattgtgatg ttactgtgtg cgggcatttc
    85621 attttcactg gcagtgattg acccggcagc ggtacacctt ttgagttggg cgggggcggc
    85681 atatattgtc tggctggcgt ggaaaatcgc caccagccca acaaaggaag acggacttca
    85741 ggcaaaacca atcagctttt gggccagctt tgctttgcag tttgtgaacg tcaaaatcat
    85801 tttgtacggt gttacggcac tgtcgacgtt tgttctgccg caaacacagg cgttaagctg
    85861 ggtagttggc gtcagcgttt tgctggcgat gattgggacg tttggcaatg tgtgctgggc
    85921 gctggcgggg catctgtttc agcgattgtt tcgccagtat ggtcgccagt taaatatcgt
    85981 gcttgccctg ttgctggtct attgcgcggt acgcattttc tattaacgaa aaaaagcgga
    86041 agaggtcgcc ctcttccgct tagtaacttg ctacttaagc cttacaggct ttcagtaaag
    86101 gtacgagcga taacgtcgcg ctgctgttcc ggagtcagag agttaaagcg aactgcataa
    86161 ccggatacac ggatggtcag ctgcggatat ttttccggat gcttaactgc atcttccaga
    86221 gtttcacgac gcagaacgtt aacgttcagg tgttgaccac cttcaacgcg aacttctggt
    86281 ttcacttcta ctggaacttc acggtattca atgtcaccca gtttgcttac tgcaaccact
    86341 tcatcttctg cataacctgc ttttgcaacg atgcaacgtg cttcgccttt ttcgctgtcc
    86401 agcagccaga aagagttcag cagatcgtcg ttagcggctt tagtaatctg gatacctgta
    86461 atcatgtgat gcctccccgg caaaattatt tgatttgttc agcctgtcgc ggccaattgg
    86521 taaaaccatt gttgcttgag tgtatatata ctcctcaacc acccttgatt ctttgattta
    86581 aatcaataaa aaccacacat cgattgtggt cgtaaatgga ttttattgtt ttacatcaac
    86641 ttatgcgggt gtgaaatttt accaatttac atttttttgc actcgtttaa gtctaaaaaa
    86701 tgagcatgat tttgttctgt agaaagaagc agttaagcta ggcggattga agattcgcag
    86761 gagagcgaga tggctaacga attaacctgg catgacgtgc tggctgaaga gaagcaacaa
    86821 ccttattttc ttaataccct tcagaccgtc gccagcgagc ggcagtccgg cgtcactatc
    86881 tatccaccac aaaaagatgt ctttaacgcg ttccgcttta ccgagttggg tgacgttaaa
    86941 gtggtgattc tcggccagga tccttatcac ggaccgggac aggcgcatgg tctggcattt
    87001 tccgttcgtc ccggcattgc cattcctccg tcattattga atatgtataa agagctggaa
    87061 aatactattc cgggcttcac ccgccctaat catggctatc tcgaaagctg ggcgcgtcag
    87121 ggcgttctgc tgctcaatac tgtgttgacg gttcgcgcag gtcaggcgca ttcccacgcc
    87181 agcctcggtt gggagacgtt tactgataaa gtcatcagcc tgattaacca gcaccgcgaa
    87241 ggcgtggtgt ttttgttgtg gggatcacat gcgcaaaaga aaggggcgat tatagataag
    87301 caacgccatc atgtactgaa agcaccgcat ccgtcgccgc tttcggcgca tcgtggattc
    87361 tttggctgca accattttgt gctggcaaat cagtggctgg aacaacgtgg cgagacgccg
    87421 attgactgga tgccagtatt accggcagag agtgagtaaa tttgcgggga aatgccggat
    87481 ggcagagttg ccacccggct gatttatcag gctttattct gacgccacca ttcaccaagc
    87541 aaaacgccgg ttgcgacaga gatattcagc ccggcaacgt tgcccgtacc gtcaatcttc
    87601 acgcgcagat cgttcggatc gcgtgcggca tccggtaacc cttcatattc ctgacccagc
    87661 accagtacca ttttcgctgg cagactagtt ttgaacagcg gtttaccctg ctcgctggaa
    87721 gtggtcacta cggtgtaacc tgcctgacgg aaatcatcca gcacgttaac aatgttgtcg
    87781 ccagtaatcg gctgaacgtg ctctgcgccg ccttctgcgg tacggatagc cgcccccgac
    87841 tccagcagtg ccgcatcctg caccacgaca cctttcacgc cgaagtgcgc gcagctgcgc
    87901 atcatgccac ccaggttatg cgggttagat tcgttttcca gtgccagaac gcaatcctgc
    87961 gcgcctgcct ggcttaccca ctgctgcacg gttgtaccgt tacgcttttt gatcaagaag
    88021 caaacgccgc cgtgatgttc cgtgcctgac gcttttgtca attccgcttc atccaccaca
    88081 tggtacgctt tgcggtttgc tgccatccag cgcaaagctt ctttaaaacg cggcgttaca
    88141 ctctggataa accaggcgcg aacaatcgct tccggacggc tctggaaaag tgcctgacat
    88201 gcattttcgc cgtagacgcg ggtttcttcc gcacgctgac gacgcaacac ttccggatca
    88261 ataaaacttt taccactgat gccaccgtga tcggcctttt ccggcgtctc atcacccggc
    88321 gcgcgggaaa ccgtgcgcca cggcgaatct tcccacttgc ggtcacgggg ctgactctgt
    88381 ttgtcatcgc gggcggggcg acggccaccg tcagcacgag attttcctgg acgcccgccc
    88441 cctttcccgg tacgcgggtt gtgggtacgt ttatcagaat catcatcact gcggacatac
    88501 atcactttga ccttgccgct tttacctttc atttcatcgt tcatgctttt ctccaccagc
    88561 gctgcgcgaa gcgcgcagat tacccgaagt ccgcgcggtt cgccatgatt tcgtaccaaa
    88621 gcctgcgact atcataccta ttgaataaaa cagattgttg tctggaacaa tgtccccgat
    88681 aatatgtaac atattagaaa cataccggcg tcgttgccga taagtctcct tactcatccc
    88741 gaggttagtt atgaataccg tttgtaccca ttgtcaggcc atcaatcgca ttcccgacga
    88801 tcggatcgaa gatgcggcaa aatgcggacg ctgcggtcac gacttgtttg acggagaggt
    88861 gattaatgcg accggtgaaa cactcgacaa attgctgaag gatgatctac ctgtggtgat
    88921 cgacttctgg gcaccgtggt gcggcccctg ccgtaatttc gcaccaattt ttgaagatgt
    88981 cgcgcaagag cgtagcggta aagtgcgctt tgtgaaagtg aataccgaag ctgaacgtga
    89041 attgagcagt cgctttggaa ttcgtagtat accgacgatc atgattttca aaaacggtca
    89101 ggttgtcgac atgcttaatg gcgcagtacc gaaagcgccg ttcgatagct ggctgaacga
    89161 atctctttaa tcttaccggg gcgcatcttg tgccccgttt tctcctctgc gacaatggcg
    89221 ttttttcgac gctctcttat gaccgaaaac gctgttctcc agttacgcgc cgagcgtatt
    89281 gcgcgcgcaa cacgtccttt tcttgcccgc ggtaatcgcg ttcgtcgctg ccaacgctgt
    89341 cttttgccag agaaattatg tctctgttcg acaattaccc cagcacaagc aaaaagtcgc
    89401 ttctgtttgc tgatgttcga caccgagcca atgaagccca gtaataccgg gcgtctcatt
    89461 gctgatattt tgcctgatac cgttgcgttt caatggtcgc gtaccgaacc ctcgcaggat
    89521 ttgctggagt tggtgcaaaa cccggactat cagccaatgg tggtctttcc cgcttcgtat
    89581 gctgatgagc aacgggaagt gatcttcaca ccacctgccg gtaagccacc gctgtttatc
    89641 atgctcgatg gtacctggcc ggaagctcgc aagatgtttc gtaaaagtcc gtatctggat
    89701 aatcttcccg tcatttccgt cgatctttcc cggctttctg cctatcgcct gcgtgaagcc
    89761 caggctgaag gccaatattg tactgccgag gtagccatcg cactgttaga tatggccggc
    89821 gataccgggg cggcggcagg gttaggcgag cattttaccc gctttaaaac acgctatctg
    89881 gcaggaaaaa cgcaacatct gggtagcatc acagcagaac agttagaaag cgtttaaaat
    89941 cattcggtca cttctgcggg agaccggtat gagtcagcga ggactggaag cactactgcg
    90001 accaaaatcg atagcggtaa ttggcgcgtc gatgaaaccc aatcgcgcag gttacctgat
    90061 gatgcgtaac ctgctggcgg gaggctttaa cggaccggta ctcccggtga cgccagcctg
    90121 gaaagcggtg ttgggtgtgt tggcctggcc ggatattgcc agcttgccct ttacacccga
    90181 ccttgcggtt ttatgtacca atgccagccg taatcttgct cttctggaag agctcggcga
    90241 gaaaggctgt aaaacctgca ttattctttc cgccccggca tcgcaacacg aagatctccg
    90301 cgcctgcgcc ctgcgccata acatgcgcct gcttggacca aacagtctgg gtttactggc
    90361 tccctggcaa ggtctgaatg ccagcttttc gcctgtgccg attaaacgcg gcaagctggc
    90421 gtttatttcg caatcggctg ccgtctccaa caccatcctc gactgggcgc aacagcgtaa
    90481 gatgggcttt tcctacttta ttgcgctcgg cgacagcctg gatatcgacg ttgatgaatt
    90541 gcttgactat ctggcacgcg acagtaaaac cagcgccatc ctgctctatc tcgaacagtt
    90601 aagcgacgcg cgacgctttg tttcggcggc ccgtagtgcc tcgcgtaata aaccgattct
    90661 ggtgattaaa agcggacgta gcccggcggc acagcgactg ctcaacacga cggcaggaat
    90721 ggacccggca tgggatgcgg ctattcagcg tgccggtttg ttgcgggtac aggacaccca
    90781 cgagctgttt tcggcggtgg aaacccttag ccatatgcgc ccgctacgtg gcgaccggct
    90841 gatgattatc agcaacggtg ctgcgcctgc cgcgctggcg ctggatgcct tatggtcacg
    90901 caatggcaag ctggcaacgc taagcgaaga aacctgccag aaactgcgcg atgcactgcc
    90961 agaacatgtg gcaatatcta acccgctcga tctacgcgat gacgccagca gtgagcacta
    91021 tattaaaacg ctggatattc tgctccacag ccaggatttt gacgcgctga tggttattca
    91081 ttcgcccagc gccgctgctc ccgcaacaga aagcgcgcaa gtattaattg aagcggtaaa
    91141 gcatcatccc cgcagcaaat atgtctcttt gctgacgaac tggtgcggcg agcactcctc
    91201 gcaagaggca cgacgtttat tcagcgaagc cgggctgccg acctaccgta ccccggaagg
    91261 aaccatcact gcttttatgc atatggtgga gtaccggcgt aatcagaagc aactacgcga
    91321 aacgccggcg ttgcccagca atctgacttc caataccgca gaagcgcatc ttctgttgca
    91381 acaggcgatt gccgaagggg ctacgtcgct cgatacccat gaagttcagc ccatcctgca
    91441 agcgtatggc atgaacacgc tccctacctg gattgccagc gatagcaccg aagcggtgca
    91501 tattgccgaa cagattggtt atccggtggc gctgaaattg cgttcgccgg atattccaca
    91561 taaatcggaa gttcagggcg tcatgcttta cctgcgtaca gccaatgaag tccagcaagc
    91621 ggcgaacgct attttcgatc gcgtaaaaat ggcctggcca caggcgcggg tccacggcct
    91681 gttggtgcaa agtatggcta accgtgctgg cgctcaggag ttgcgggttg tggttgagca
    91741 cgatccggtt ttcgggccgt tgatcatgct gggtgaaggc ggtgtggagt ggcgtcctga
    91801 agatcaagcc gtcgtcgcac tgccgccgct gaacatgaac ctggcccgct atctggttat
    91861 tcaggggatc aaaagtaaaa agattcgtgc gcgcagtgcg ctacgcccat tggatgttgc
    91921 aggcttgagc cagcttctgg tgcaggtttc caacttgatt gtcgattgcc cggaaattca
    91981 gcgtctggat attcatcctt tgctggcttc tggcagtgaa tttaccgcgc tggatgtcac
    92041 gctggatatc tcgccgtttg aaggcgataa cgagagtcgg ctggcagtgc gcccttatcc
    92101 gcatcagctg gaagaatggg tagaattgaa aaacggtgaa cgctgcttgt tccgcccgat
    92161 tttgccagaa gatgagccac aacttcagca attcatttcg cgagtcacca aagaagatct
    92221 ttattaccgc tactttagcg agatcaacga atttacccat gaagatttag ccaacatgac
    92281 acagatcgac tacgatcggg aaatggcgtt tgtagcggta cgacgtattg atcaaacgga
    92341 agagatcctc ggcgtcacgc gtgcgatttc cgatcctgat aacatcgatg ccgaatttgc
    92401 tgtactggtt cgctcggatc tcaaagggtt aggcttaggt cgacgcttaa tggaaaagtt
    92461 gattacctat acgcgagatc acggactaca acgtctgaat ggtattacga tgccaaacaa
    92521 tcgtggcatg gtggcgctag cccgcaagct cgggtttaac gttgatatcc agctcgaaga
    92581 ggggatcgtt gggcttacgc taaatcttgc ccagcgcgag gaatcatgag taaggtactg
    92641 gaaatgttga ccacttaatc gggactggtg ttattattgc ccgcttatgt cgtctgcatt
    92701 gcacagagga cccttcaatg aacagagaag aaatgcactg tgatgttgtc aaaatttaag
    92761 cgtaataaac atcaacaaca ccttgcccaa ctacccaaga tttctcaatc agttgatgat
    92821 gtcgatttct tttacgctcc cgccgacttc cgggagacgc tgctggaaaa aatagccagc
    92881 gcgaagcagc gcatttgcat tgtcgccctg tatctcgaac aggatgacgg tggcaaaggc
    92941 attctgaacg cgttgtatga ggctaaaagg cagcgtccgg aactggatgt gcgggtgctg
    93001 gtcgactggc atcgtgcaca acgtggacgc attggcgctg cggcatctaa cactaacgct
    93061 gactggtact gccgcatggc gcaggaaaat ccgggcgtag atgttccggt ttatggcgtt
    93121 ccaatcaata ctcgtgaagc ccttggtgtt ctgcacttta aaggctttat catcgacgat
    93181 agcgtacttt atagcggtgc cagcctgaac gatgtttacc tgcatcagca cgataaatat
    93241 cgctacgacc gttatcatct gatccgtaac cgtaagatgt cagacattat gtttgaatgg
    93301 gttacacaga atattatgaa tggccgcggc gttaatcgtc tggatgatgt taatcggcca
    93361 aaaagcccgg aaatcaagaa cgatattcgt ctgttccgcc aggagctgcg tgatgccgct
    93421 tatcatttcc agggcgatgc cgacaacgat cagctttctg taacgccgct agtggggctg
    93481 gggaaatcga gtctgttgaa caagaccatt ttccatctta tgccttgtgc cgagcagaaa
    93541 ctaaccatct gtacgccata cttcaacctg ccagcaatcc ttgtgcgcaa tattatccag
    93601 ttgctgcgcg aagggaaaaa ggtcgaaatt attgttggtg ataaaaccgc gaatgacttc
    93661 tacattccgg aagatgaacc tttcaagata attggcgcat tgccttatct ctatgagatc
    93721 aatctgcgtc gtttcctgag ccgtttgcag tattacgtca atactgacca gctagtggtt
    93781 cggttatgga aagatgacga caacacctat cacctgaaag ggatgtgggt tgatgataag
    93841 tggatgttga tcaccggtaa taacctgaac ccgcgcgcct ggcgtctgga tctggaaaac
    93901 gccattttga tccacgatcc gcaacttgag ctggcgccac agcgagagaa agaactggag
    93961 ctgatccgcg agcataccac catcgttaag cactatcgcg atctgcaaag tattgccgat
    94021 tatccggtga aggttcgtaa actcatccgc cgtttgcgcc gtatccgcat cgaccgatta
    94081 attagccgca tcctgtaatc acaaccccgt cctgtacggg gtttgttttt tggaggccac
    94141 gttttgcgta ttctttttgt ctgttcgcta ttgttacttt ctggatgcag ccatatggct
    94201 aacgatagct ggagcgggca ggataaagct caacacttta tcgcctcggc gatgctttcc
    94261 gccgccggaa atgaatattc acagcatcag gggatgagcc gggatcgcag tgccatgttt
    94321 ggattgatgt tctctgtcag tttgggggcg tcaaaagagc tttgggatag ccgccccgaa
    94381 gggagcggct ggagctggaa ggatttggcc tgggatgtcg ccggtgcaag caccggctat
    94441 accgtctggc aactgacccg tcactaaaga cgcatcccct tccctttgcg atgtagcatc
    94501 aaagaaacca gaaacgccac cacggccatc aaggtcacat accagaagaa ggctgtttcc
    94561 attcctattg atttcagcga caacgctacg tactccgccg aaccaccaaa tatagcatta
    94621 gcgaccgcat atgacagacc aacgcctaat gcgcgaacct gtgccgggaa catctcagcc
    94681 ttcagtattc cactgattga tgtataaaaa ctcactatca gcagggcaca catcaccaga
    94741 ccaaaagcgg cataaggcga ggaaacgttt tgcaatgctg agagaatagg aacggtaaaa
    94801 atggctgcca gcgaaccgaa acataacatt gaggtacggc gaccaatctt atccgacagc
    94861 gcgccaatga gtggttgaat aagcatgaat acaaacaatg cggcagtcat aatgccactc
    94921 gccacgttgg catgcattcc cgcagtattt accagatact tctgcatata agtagtgaag
    94981 gtatagaaac aaagggagcc cgcagcggta aaaccgagaa ccatgatgaa tgcacggcga
    95041 ttgcgccata atcctttcag agatccagct tcttttaaag cgcgcgtttc ttgttgcgaa
    95101 gtttcatcta actgacgacg taaccacaac gccacaacag ctaacacagc tcctaacgcg
    95161 aaaggaatac gccatcccca ctctctgagt gcagcgtctt ccatggtgtg ttgtaaaacc
    95221 acgacaacca gtagggctag cagttgtccg ccgatcaacg tcacatactg aaatgatgcg
    95281 taaaaacctt tgcgcccttc aacggcaact tcactcatat aggtggcgct ggtgccatat
    95341 tctccgccaa cagataatcc ctgaaataaa cgagcgagaa gcaataatgc cggagcccac
    95401 gtacctatag tttcataacc tgggaggcag gcgataacca gcgatccgaa acacatcata
    95461 cacaccgata acagcatcga ttttttgcga ccatgtttat cggctatgcg gccaaatagc
    95521 caaccgccta ttgggcgcat caggaatccc gcagcaaaaa cacctgctgt ttgtagtagt
    95581 tgagtcgtcg tgttcccgga agggaagaag atgtgggcaa agtagagtga acagaacgag
    95641 tagacataga aatcgaacca ctcgaccaga ttacctgaag aggcccccac aatcgcccaa
    95701 atgcggcgac gagtatcact acttgtcagt ttgctgtctg ccgttacagt actttcagcc
    95761 atgccattat gtctcctgcc gtaatccgat gcttttgtcg gtcgcttttg tttatttttt
    95821 tggcaaagga aatgttatac atttgttgca tatcattatg caaccttaac catgaattta
    95881 gttagcagga aaatggttat tgaggagctt aaggataaat ttctggtaag gaggacacgt
    95941 atggaagtgg gcaagttggg gaagccgtat ccgttgctga atctggcata tgtgggagta
    96001 taagacgcgc agcgtcgcat caggcatttt tttctgcgcc aatgcaaaaa ggccatccgt
    96061 caggatggcc tttcgcataa tttgatgcct ggcagttccc tactctcgca tggggagacc
    96121 ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga
    96181 ccaccgcgct acggccgcca ggcaaattct gttttatcag accgcttctg cgttctgatt
    96241 taatctgtat caggctgaaa atcttctctc atccgccaaa acagcttcgg cgttgtaagg
    96301 ttaagcctca cggttcatta gtaccggtta gctcaacgca tcgctgcgct tacacacccg
    96361 gcctatcaac gtcgtcgtct tcaacgttcc ttcaggaccc ttaaag
//
LOCUS       DAJOSY010000003        85324 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJOSY010000003 DAJOSY010000000
VERSION     DAJOSY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.003"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            4..171
                     /locus_tag="OHN27_000206"
     CDS             4..171
                     /locus_tag="OHN27_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCQ2284717.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            415..1803
                     /gene="frlA"
                     /locus_tag="OHN27_000207"
     CDS             415..1803
                     /gene="frlA"
                     /locus_tag="OHN27_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCQ2284718.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            1824..2846
                     /gene="frlB"
                     /locus_tag="OHN27_000208"
     CDS             1824..2846
                     /gene="frlB"
                     /locus_tag="OHN27_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCQ2284719.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2896..3726
                     /gene="frlC"
                     /locus_tag="OHN27_000209"
     CDS             2896..3726
                     /gene="frlC"
                     /locus_tag="OHN27_000209"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCQ2284720.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3723..4508
                     /gene="frlD"
                     /locus_tag="OHN27_000210"
     CDS             3723..4508
                     /gene="frlD"
                     /locus_tag="OHN27_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCQ2284721.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAIAQGTACAAKTIQYHGAW"
     gene            4608..5339
                     /gene="phoB"
                     /locus_tag="OHN27_000211"
     CDS             4608..5339
                     /gene="phoB"
                     /locus_tag="OHN27_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCQ2284722.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5491..6576)
                     /locus_tag="OHN27_000212"
     CDS             complement(5491..6576)
                     /locus_tag="OHN27_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCQ2284723.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAETGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6588..7892)
                     /locus_tag="OHN27_000213"
     CDS             complement(6588..7892)
                     /locus_tag="OHN27_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCQ2284724.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFVGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7904..8257)
                     /locus_tag="OHN27_000214"
     CDS             complement(7904..8257)
                     /locus_tag="OHN27_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCQ2284725.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8268..9146)
                     /locus_tag="OHN27_000215"
     CDS             complement(8268..9146)
                     /locus_tag="OHN27_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCQ2284726.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGTSEGKITPLEEKVFIAAALAYNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9143..10369)
                     /locus_tag="OHN27_000216"
     CDS             complement(9143..10369)
                     /locus_tag="OHN27_000216"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCQ2284727.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10369..11532)
                     /locus_tag="OHN27_000217"
     CDS             complement(10369..11532)
                     /locus_tag="OHN27_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCQ2284728.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFALKVLPEIVAEIQNLPGLHLAGLTHFPCLLWDEAVGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(11616..11978)
                     /locus_tag="OHN27_000218"
     CDS             complement(11616..11978)
                     /locus_tag="OHN27_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCQ2284729.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11995..12900)
                     /locus_tag="OHN27_000219"
     CDS             complement(11995..12900)
                     /locus_tag="OHN27_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2284730.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELANECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13190..14194)
                     /gene="trpS"
                     /locus_tag="OHN27_000220"
     CDS             complement(13190..14194)
                     /gene="trpS"
                     /locus_tag="OHN27_000220"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCQ2284731.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14187..14945)
                     /gene="gph"
                     /locus_tag="OHN27_000221"
     CDS             complement(14187..14945)
                     /gene="gph"
                     /locus_tag="OHN27_000221"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCQ2284732.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14938..15615)
                     /gene="rpe"
                     /locus_tag="OHN27_000222"
     CDS             complement(14938..15615)
                     /gene="rpe"
                     /locus_tag="OHN27_000222"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCQ2284733.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15633..16469)
                     /gene="dam"
                     /locus_tag="OHN27_000223"
     CDS             complement(15633..16469)
                     /gene="dam"
                     /locus_tag="OHN27_000223"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCQ2284734.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16576..17862)
                     /gene="damX"
                     /locus_tag="OHN27_000224"
     CDS             complement(16576..17862)
                     /gene="damX"
                     /locus_tag="OHN27_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCQ2284735.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17954..19042)
                     /gene="aroB"
                     /locus_tag="OHN27_000225"
     CDS             complement(17954..19042)
                     /gene="aroB"
                     /locus_tag="OHN27_000225"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCQ2284736.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19099..19620)
                     /gene="aroK"
                     /locus_tag="OHN27_000226"
     CDS             complement(19099..19620)
                     /gene="aroK"
                     /locus_tag="OHN27_000226"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCQ2284737.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(20021..21259)
                     /gene="hofQ"
                     /locus_tag="OHN27_000227"
     CDS             complement(20021..21259)
                     /gene="hofQ"
                     /locus_tag="OHN27_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCQ2284738.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21171..21575)
                     /gene="hofP"
                     /locus_tag="OHN27_000228"
     CDS             complement(21171..21575)
                     /gene="hofP"
                     /locus_tag="OHN27_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCQ2284739.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21565..22005)
                     /gene="hofO"
                     /locus_tag="OHN27_000229"
     CDS             complement(21565..22005)
                     /gene="hofO"
                     /locus_tag="OHN27_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCQ2284740.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21989..22528)
                     /gene="hofN"
                     /locus_tag="OHN27_000230"
     CDS             complement(21989..22528)
                     /gene="hofN"
                     /locus_tag="OHN27_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCQ2284741.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22528..23307)
                     /gene="hofM"
                     /locus_tag="OHN27_000231"
     CDS             complement(22528..23307)
                     /gene="hofM"
                     /locus_tag="OHN27_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCQ2284742.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23427..25979
                     /gene="mrcA"
                     /locus_tag="OHN27_000232"
     CDS             23427..25979
                     /gene="mrcA"
                     /locus_tag="OHN27_000232"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCQ2284743.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGHQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26147..26707)
                     /gene="nudE"
                     /locus_tag="OHN27_000233"
     CDS             complement(26147..26707)
                     /gene="nudE"
                     /locus_tag="OHN27_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCQ2284744.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            27027..29162
                     /gene="igaA"
                     /locus_tag="OHN27_000234"
     CDS             27027..29162
                     /gene="igaA"
                     /locus_tag="OHN27_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCQ2284745.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29227..29895
                     /gene="yrfG"
                     /locus_tag="OHN27_000235"
     CDS             29227..29895
                     /gene="yrfG"
                     /locus_tag="OHN27_000235"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCQ2284746.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29906..30307
                     /gene="hslR"
                     /locus_tag="OHN27_000236"
     CDS             29906..30307
                     /gene="hslR"
                     /locus_tag="OHN27_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCQ2284747.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30332..31210
                     /gene="hslO"
                     /locus_tag="OHN27_000237"
     CDS             30332..31210
                     /gene="hslO"
                     /locus_tag="OHN27_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCQ2284748.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31273..32997)
                     /locus_tag="OHN27_000238"
     CDS             complement(31273..32997)
                     /locus_tag="OHN27_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCQ2284749.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATNWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSNAEYMKDPIRARDLLMALDGEQHLQKVVSEKS
                     LAENVLIAPGSGKPDAAFWSALIKERYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLNVNLNE"
     gene            33376..34998
                     /gene="pckA"
                     /locus_tag="OHN27_000239"
     CDS             33376..34998
                     /gene="pckA"
                     /locus_tag="OHN27_000239"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCQ2284750.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35074..36426)
                     /gene="envZ"
                     /locus_tag="OHN27_000240"
     CDS             complement(35074..36426)
                     /gene="envZ"
                     /locus_tag="OHN27_000240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCQ2284751.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36423..37142)
                     /gene="ompR"
                     /locus_tag="OHN27_000241"
     CDS             complement(36423..37142)
                     /gene="ompR"
                     /locus_tag="OHN27_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCQ2284752.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37370..37846
                     /gene="greB"
                     /locus_tag="OHN27_000242"
     CDS             37370..37846
                     /gene="greB"
                     /locus_tag="OHN27_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCQ2284753.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37943..40264
                     /locus_tag="OHN27_000243"
     CDS             37943..40264
                     /locus_tag="OHN27_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCQ2284754.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDTAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40702..40929
                     /gene="feoA"
                     /locus_tag="OHN27_000244"
     CDS             40702..40929
                     /gene="feoA"
                     /locus_tag="OHN27_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCQ2284755.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40946..43267
                     /gene="feoB"
                     /locus_tag="OHN27_000245"
     CDS             40946..43267
                     /gene="feoB"
                     /locus_tag="OHN27_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCQ2284756.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43267..43503
                     /gene="feoC"
                     /locus_tag="OHN27_000246"
     CDS             43267..43503
                     /gene="feoC"
                     /locus_tag="OHN27_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCQ2284757.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLHTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43706..44584
                     /gene="rpnA"
                     /locus_tag="OHN27_000247"
     CDS             43706..44584
                     /gene="rpnA"
                     /locus_tag="OHN27_000247"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCQ2284758.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(44611..45381)
                     /gene="bioH"
                     /locus_tag="OHN27_000248"
     CDS             complement(44611..45381)
                     /gene="bioH"
                     /locus_tag="OHN27_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCQ2284759.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            45419..46102
                     /gene="gntX"
                     /locus_tag="OHN27_000249"
     CDS             45419..46102
                     /gene="gntX"
                     /locus_tag="OHN27_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCQ2284760.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46161..46736
                     /gene="nfuA"
                     /locus_tag="OHN27_000250"
     CDS             46161..46736
                     /gene="nfuA"
                     /locus_tag="OHN27_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCQ2284761.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47097..48413
                     /gene="gntT"
                     /locus_tag="OHN27_000251"
     CDS             47097..48413
                     /gene="gntT"
                     /locus_tag="OHN27_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCQ2284762.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48458..50542)
                     /gene="malQ"
                     /locus_tag="OHN27_000252"
     CDS             complement(48458..50542)
                     /gene="malQ"
                     /locus_tag="OHN27_000252"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCQ2284763.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50552..52945)
                     /gene="malP"
                     /locus_tag="OHN27_000253"
     CDS             complement(50552..52945)
                     /gene="malP"
                     /locus_tag="OHN27_000253"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCQ2284764.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWEVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            53557..56262
                     /gene="malT"
                     /locus_tag="OHN27_000254"
     CDS             53557..56262
                     /gene="malT"
                     /locus_tag="OHN27_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCQ2284765.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREDTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQDAVQHAQQLLKMMGYGV"
     gene            complement(56305..57320)
                     /gene="rtcA"
                     /locus_tag="OHN27_000255"
                     /pseudo
     CDS             complement(56305..57320)
                     /gene="rtcA"
                     /locus_tag="OHN27_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
     gene            complement(57324..58550)
                     /gene="rtcB"
                     /locus_tag="OHN27_000256"
     CDS             complement(57324..58550)
                     /gene="rtcB"
                     /locus_tag="OHN27_000256"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCQ2284766.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTRKTVRQQQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGARRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            58739..60337
                     /gene="rtcR"
                     /locus_tag="OHN27_000257"
     CDS             58739..60337
                     /gene="rtcR"
                     /locus_tag="OHN27_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCQ2284767.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSVLTQLLGAEAENIDLFDRLQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(60319..61077)
                     /locus_tag="OHN27_000258"
     CDS             complement(60319..61077)
                     /locus_tag="OHN27_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCQ2284768.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(61094..61924)
                     /gene="glpG"
                     /locus_tag="OHN27_000259"
     CDS             complement(61094..61924)
                     /gene="glpG"
                     /locus_tag="OHN27_000259"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCQ2284769.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(61969..62295)
                     /gene="glpE"
                     /locus_tag="OHN27_000260"
     CDS             complement(61969..62295)
                     /gene="glpE"
                     /locus_tag="OHN27_000260"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCQ2284770.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            62485..63990
                     /gene="glpD"
                     /locus_tag="OHN27_000261"
     CDS             62485..63990
                     /gene="glpD"
                     /locus_tag="OHN27_000261"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCQ2284771.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLAESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(64042..64647)
                     /locus_tag="OHN27_000262"
     CDS             complement(64042..64647)
                     /locus_tag="OHN27_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2284772.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(64650..65417)
                     /locus_tag="OHN27_000263"
     CDS             complement(64650..65417)
                     /locus_tag="OHN27_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2284773.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDETVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(65420..66130)
                     /locus_tag="OHN27_000264"
     CDS             complement(65420..66130)
                     /locus_tag="OHN27_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2284774.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(66146..67654)
                     /locus_tag="OHN27_000265"
     CDS             complement(66146..67654)
                     /locus_tag="OHN27_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCQ2284775.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(67779..70226)
                     /gene="glgP"
                     /locus_tag="OHN27_000266"
     CDS             complement(67779..70226)
                     /gene="glgP"
                     /locus_tag="OHN27_000266"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCQ2284776.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(70245..71678)
                     /gene="glgA"
                     /locus_tag="OHN27_000267"
     CDS             complement(70245..71678)
                     /gene="glgA"
                     /locus_tag="OHN27_000267"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCQ2284777.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(71678..72973)
                     /gene="glgC"
                     /locus_tag="OHN27_000268"
     CDS             complement(71678..72973)
                     /gene="glgC"
                     /locus_tag="OHN27_000268"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCQ2284778.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(72991..74964)
                     /gene="glgX"
                     /locus_tag="OHN27_000269"
     CDS             complement(72991..74964)
                     /gene="glgX"
                     /locus_tag="OHN27_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCQ2284779.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(74961..77147)
                     /gene="glgB"
                     /locus_tag="OHN27_000270"
     CDS             complement(74961..77147)
                     /gene="glgB"
                     /locus_tag="OHN27_000270"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCQ2284780.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(77420..78523)
                     /gene="asd"
                     /locus_tag="OHN27_000271"
     CDS             complement(77420..78523)
                     /gene="asd"
                     /locus_tag="OHN27_000271"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCQ2284781.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            78715..79308
                     /gene="yhgN"
                     /locus_tag="OHN27_000272"
     CDS             78715..79308
                     /gene="yhgN"
                     /locus_tag="OHN27_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCQ2284782.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(79365..80705)
                     /gene="gntU"
                     /locus_tag="OHN27_000273"
     CDS             complement(79365..80705)
                     /gene="gntU"
                     /locus_tag="OHN27_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCQ2284783.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(80709..81236)
                     /gene="gntK"
                     /locus_tag="OHN27_000274"
     CDS             complement(80709..81236)
                     /gene="gntK"
                     /locus_tag="OHN27_000274"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCQ2284784.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(81375..82370)
                     /gene="gntR"
                     /locus_tag="OHN27_000275"
     CDS             complement(81375..82370)
                     /gene="gntR"
                     /locus_tag="OHN27_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCQ2284785.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(82594..83289)
                     /gene="yhhW"
                     /locus_tag="OHN27_000276"
     CDS             complement(82594..83289)
                     /gene="yhhW"
                     /locus_tag="OHN27_000276"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCQ2284786.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(83412..84449)
                     /locus_tag="OHN27_000277"
     CDS             complement(83412..84449)
                     /locus_tag="OHN27_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCQ2284787.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALTKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFSADDSVGV
                     LEYVNDEGVTVREEIKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            84782..85270
                     /gene="yhhY"
                     /locus_tag="OHN27_000278"
     CDS             84782..85270
                     /gene="yhhY"
                     /locus_tag="OHN27_000278"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2284788.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDYMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSHWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT        20550 a        23292 c        21472 g        20010 t
ORIGIN      
        1 taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac gttaactgca
       61 tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct gctgcacccg
      121 gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta agtctctgac
      181 agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg agggcgtatt
      241 tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa ttcaatttga
      301 taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct tcctccacat
      361 tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt ttaaatgact
      421 gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctatggg aagccaggaa
      481 ctccaacgca agctcggatt ttgggccgtt cttgcaatcg ccgtcgggac aaccgtcggc
      541 tccggtattt ttgtatctgt gggtgaagtg gcaaaagcag cgggcacgcc gtggcttacg
      601 gtgctcgcgt ttgtcattgg cgggttaatt gtgatcccgc aaatgtgcgt ctatgcggaa
      661 ctatccaccg cttatccgga aaatggcgca gattatgttt atctgaaaaa tgccggaagc
      721 cgaccgctgg ctttcctctc cggctgggcc agcttctggg ccaacgatgc gccgtcattg
      781 tcgattatgg cgctggcgat tgtcagcaat cttggctttt taacgcctat cgatccgttg
      841 ctcggtaaat ttatcgccgc cggattaatt atcgccttta tgttgctaca cctgcgctcc
      901 gttgaaggcg gcgcagcgtt tcagacgcta attaccatcg ccaaaattat cccgttcact
      961 atcgtcattg gccttgggat cttctggttt aaagcggaga attttgccgc ccctaccacc
     1021 actgcgattg gcgcaacggg cagctttatg gcgctgctgg cggggatctc tgccaccagt
     1081 tggtcgtata ccggcatggc ctctatctgt tatatgaccg gcgaaattaa aaaccccgga
     1141 aaaaccatgc cacgagcgct gattggttcc tgtctgctgg ttctggtgct ctacaccctg
     1201 ctggcgctgg tgatttccgg cctgatgccc ttcgacaaac tcgccaattc tgaaacgccg
     1261 atttccgacg ccctgacctg gatccccgca ctcggcagca ccgctgggat ctttgttgcc
     1321 atcacggcga tgatcgtcat tcttggttcg ctttccagct gcgtgatgta ccagccgcgg
     1381 ctggaatacg cgatggcgaa agacaacctg ttctttaaat gcttcggcca tgtgcatccg
     1441 aaatacaaca cgccggatgt ctccatcatc ctgcaagggg cgctggggat cttcttcatc
     1501 ttcgtttccg atctcaccag cctgctgggt tatttcaccc tggtgatgtg tttcaaaaat
     1561 accctcacct tcggctccat catctggtgt cgtaaacgcg acgattacaa accgctgtgg
     1621 cgtactccgg ctttcgggct gatgaccacc ctcgccattg cgtcaagcct cattctggtc
     1681 gcctcaacct ttgtctgggc accgattccc ggccttatct gcgccgtcat cgttattgct
     1741 actggtctgc ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa cgctttgtcg
     1801 taatgttatc tggagaaaat aaaatgttgg atattgataa aagcaccgtg gactttctgg
     1861 tcaccgaaaa tatggttcag gaagtggaaa aagttctcag ccatgacgtt ccgctggtgc
     1921 acgccatcgt ggaagagatg gtgaagcgcg acattgatcg tatttatttc gttgcctgcg
     1981 gatcgccact caacgcggcg caaacggcga aacatctggc ggatcgcttt tccgatcttc
     2041 aggtctacgc catttccggc tgggagttct gcgataacac cccgtatcgc ctcgacgatc
     2101 gttgcgcagt aattggcgtt tctgactacg gtaaaaccga agaggtaatc aaagcgctgg
     2161 agctgggccg ggcctgcggc gcactcactg cggcgttcac caaacgcgcg gatagcccga
     2221 ttacctcggc ggcggaattt agcattgatt atcaggccga ctgtatctgg gaaattcacc
     2281 tgctgctctg ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg aacgcggaaa
     2341 tcggcaagat caaaaacgat ctcaagcagt tgccgaatgc gctcggtcat ctggtacgca
     2401 cctgggaaga aaaaggccgc cagcttggtg aactggccag ccagtggccg atgatttata
     2461 ccgttgctgc gggtccgctg cgtccgctgg gttacaaaga aggcattgta acgctgatgg
     2521 aatttacctg gacgcacggc tgcgtgattg agagcggaga gttccgccat ggcccgctgg
     2581 agattgtcga accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa agtcgccaca
     2641 ccaccgaacg cgccattaac tttgttaaac agcgtactga caacgtgatc gtcatcgatt
     2701 acgccgaaat ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg ttcgtgccaa
     2761 tggagtggct ctgctactac ctgtctattt acaaagatca caacccggat gaacgccgct
     2821 attacggtgg tctggtggaa tattaatccc tctcccggcc cggcaacggg ccggatttac
     2881 gcaaggagtt acccgatgaa aacaggtatg tttacctgcg gccaccagcg gctgcctatt
     2941 gaacacgcat ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat ttggggcggt
     3001 cgcccgcacg cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat caaggcgctg
     3061 gcgcagacgt atcagatgcc gattatcggc tatacgccag aaaccaacgg ctatccgtat
     3121 aacatgatgc tgggcgatga acatatgcgt cgcgaaagcc tcgacatgat caagctggcg
     3181 atggatatgg caaaagagat gaacgcgggt tatacgctga tttccgcggc ccacgcgggc
     3241 tatctcacgc cacctaatgt tatctgggga cggctggcag agaacctaag cgaactgtgt
     3301 gagtacgcgg aaaacatcgg catggatcta atcctcgaac cgttaacgcc gtatgaatcg
     3361 aacgtcgtgt gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc ttcgccgcgc
     3421 ctgttcagca tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt gatgagttat
     3481 ttcgacaaac tgggcgataa attacgtcat ctgcatattg tcgacagcga cggggccagc
     3541 gacacgcatt acattcctgg agaaggcaaa atgccgctgc gggaactgat gcgcgatatt
     3601 attgagcggg gctatgaggg ttactgtacg gtggagctgg tgacgatgta tatgaacgag
     3661 cccagactct atgcccgcca ggcgctggaa cgctttcgcg cgctgctgcc ggaggatgag
     3721 agatgaaaac cctggcgaca atcggcgata actgcgtcga tatctacccg caactgaata
     3781 aagcgttttc tggcggtaat gcggtcaatg tggcggtgta ctgcactcgc tacggcatac
     3841 agccgggatg cattacctgg gtgggtgacg atgactacgg cacaaagctg aagcaggatc
     3901 tcgcccgcat gggcgtcgat atcagccatg tccatacgaa acacggcgtt accgcacaaa
     3961 ctcaggtgga actgcacgac aatgatcgcg tttttggcga ctacaccgaa ggcgtgatgg
     4021 ccgactttgc cctgagtgaa gaggattacg cctggctggc gcagtatgac attgtgcacg
     4081 cggcaatctg gggacatgcg gaagacgcat tcccacagct gcacgctgcg ggcaaactta
     4141 ccgctttcga cttctccgac aagtgggaca gcccgctctg gcagacactg gtgccgcatc
     4201 tcgattttgc ctttgcctcc gcaccgcaag aagacgaaac gctgcgtctg aagatgaaag
     4261 cgattgttgc ccgtggcgca ggaacagtga ttgtcacgct gggtgaaaac ggcagcattg
     4321 cctgggatgg cgcgcagttc tggcgtcagg ctcctgaacc ggtgacggtt atcgacacca
     4381 tgggtgccgg agattcgttc attgccggat tcctttgcgg ctggtctgcg gggatgacat
     4441 taccgcaggc gatagcgcag ggaacggcgt gcgcggcgaa aaccattcag taccacggtg
     4501 cctggtaggt ataacgttgg cgtgagcatc ttcacgccaa cgtgctgtta cttgccggaa
     4561 aacgacccta taatccgagt aattcattct ttatttcagg gtcgattatg tcagctacgg
     4621 accgctactc tcatcaactc ctctacgcta ccgtccgcca gcgactgctg gatgatatcg
     4681 cgcagggggt ttaccaggcc gggcaacaga tccctaccga aaacgagctt tgtacacaat
     4741 ataacgtcag ccgcattacc attcgcaaag ccatcagcga cttagtggca gacggcgtac
     4801 tgatccgctg gcagggaaaa ggcacctttg tacaaagcca gaaagttgaa aacgccctgc
     4861 ttactgtcag tggttttacc gattttggcg tctcacaagg caaggcgacg aaagagaaag
     4921 tgatcgaaca ggaacgggtc agcgccgcgc cgttttgcga aaagctgaac atccccggaa
     4981 acagcgaagt gttccatctc tgccgggtga tgtatctcga taaagagccg ctgtttattg
     5041 atagttcatg gatcccgctg tcgcgttatc ctgactttga tgagatttac gtcgaaggaa
     5101 gctccaccta tcagttattt caggagcgtt ttgacacgcg agtggtcagc gacaaaaaga
     5161 ccatcgatat ctttgccgcc acccgcccgc aggcaaaatg gctgaaatgc gaactgggcg
     5221 aaccgttgtt tcgcatcagc aaaatcgcct ttgatcagaa tgacaaaccg gtgcacgtct
     5281 ccgaactctt ctgccgcgcc aatcgcatca ccttaactat tgataataaa agacattaac
     5341 cgtaggccgg ataagatgcg ccagcatcgc atccggcgat gctggcgcgt tgaattttac
     5401 atcccgtacg ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgttcagta
     5461 cggaatttag cttgacggtg ctatccagca ttaaatactg gcaatactct cacgcaaaat
     5521 ccgcagcacc gtctcttcac cgctgcgatt cgggttaatg cgaatcgcac aatgttctga
     5581 ttgtggattc gcctggcgaa acgttccgga aaggcgataa aagagcggcg ggatttcata
     5641 tttcgactct gcacccaccg ggtaaggcaa ggcaccgcgc ttttgcgcct cttccagcac
     5701 tctggcggca atcggctgat gaaactcgac aatcaacacc ttcgactgcg cattagcaat
     5761 caccgcgctt ttcacttccg gcaccgcacc accgttaagc aaagccagca accgttcaga
     5821 tacccctgcc tgcaccgcgt gcatcactgg cgcaaacacc agaccacgca atacttccag
     5881 cgcctgtgcg ccctggatct ggctaccgcc ggagtaaagc gtggcgcgaa tacggttgat
     5941 aacatcagca tcgccgacca ctgcaccaac accctctggc ccaaatagct tgaagcagga
     6001 aaatgtcgag acattcgcgc cgcattcaca gccgattcga gccaccttca tcaccgcata
     6061 gttgtcatcg gttaacgctg gaacacctgc cgcgcgcaac gttgccagca catctgccag
     6121 cacgtagctg tcctgcggct gctggcgcgt atgctgcacc agcgccgcat ccggttgttg
     6181 ctcgtcgacg acctgcttca gtgccgacag gtcattgaaa tcaacagtaa taagcgtcag
     6241 ccccatctgc tcaataataa cccgtgtcgt cgggtaaaca ggcgcgtcat gcaccagaag
     6301 acgctgcccc ggtttgagca aagccgccag cccggcgcga atcgcgccag tctccgcgcc
     6361 ctgcaccagc gccgcagcct gtgcgtgaaa tgcatcagcc agcacctgtt ccacacgctg
     6421 ggtaacgcgc ggttgattca gtcctggcgt taagcctaaa tcaccgccag taagaaactc
     6481 gctgccgggg aaatggcgac aaatgctatc caccagcgca aactgctttt gctgcgcctc
     6541 aataatcgtc aggctttgca gaggaaacgt cttcataggt gccttcctta agccggaacg
     6601 aacaggccaa gccagtaaag aacattcagc agaataccgg tgatcattac tgccacgacc
     6661 ggtgccgcca ttttctgtac cggacggccc agggattcgt tgaggaagta aatggcaacc
     6721 gcgatagaga atccggtata acccgccatc ttaattgctg cgaaaatcga accgaccagc
     6781 agcgccactt ccatcagcat attcatggcg ttacggatgt tatccgacgc attacgcacc
     6841 gacgggtagc gtcccagcca tttgccgatc gaacgaagca gcaagacttc cgccgaaata
     6901 accactgcgc ctaataccgc tgcaaccatc ggattcggcg agagatagcc caccgcataa
     6961 acaaaggtaa agcccgcaac tgcatacaca cccgttgcta acgcggtggt ggcaatcaac
     7021 ggcacaaacc ccagtccgcg cataaattct gccagagccg cctgattaat cagcgtttgc
     7081 gattgttccg gcgttacgcc tgcggaatat gctttctcca gtgtgaagat cgacacttca
     7141 ctgccaacaa aaatcttcat gctggcaacg gcggcaatca atgctcccac gatggcgata
     7201 tagggtaagt ttttgataat ccgtgacgtg cgttcttcaa acaccgaaag cccgctggca
     7261 tcaatgtcat tttcatcacg atgacgcagg tcgtgagtta tcgcgatccc cagcagcatc
     7321 accatgccaa taaagatttc gatggattca gggttaagat gtgggaaata gcgcacgact
     7381 accacacggg tcatcagtac caccacggcg gcgatcagac tttgcttcca gccaaactgg
     7441 tagaaaatcg ccaccagcgg gaacagtgca aaagctgaaa ccaccggcga gcttaattcc
     7501 cccaggctac ctaatacatc caccggcagc gcggtcagca gctggtttac tggcaacagg
     7561 caagtaagga tcaacacgcc ccagatagcg ccaagaccaa acgccatcag gctgtttatc
     7621 gccaggacgc cgaggatatc ggtaggaaga aagagtaacc atgcgttgag cagcccggtt
     7681 ttcagggtaa aagagatccc caccgaggcc acaaaaccaa tgctcagacc aaaagcgata
     7741 ctccccgcct cgcgacggtt catatagcct tcaatcagtt gcggcaggat cgggcggatg
     7801 ccgtcatgaa aaacagccgc cgagcgatgc gccagaagcg atgtcatacc cgtcaggcac
     7861 gccaccacga taatctgaat atacagatcc atagtcgtgc cttttatttc agatggttaa
     7921 tcagcatcgg aatcgcgtgt tcgacgtgct caacggaaag gccaaacgcc acttttcctt
     7981 cagcgatcat tttggcgata tgctcgtctt tcgctttaat gcctggtttg gcaatggtgc
     8041 aacttttgtt atagcctatc accgcgatgg caatcgacaa tgccgcgccc gcaccggtat
     8101 tacacgcgcc gatgtaataa tccagttgcc cggatttcac cttcattgcc gcttccatgt
     8161 cgttgtgaat gaaaacttca aaacagccag gagccgtcgc ttcaatagtt tttttaatct
     8221 gctcacgctg taagcctgca acgccaatct ttttcatgag tctgtcctta ttggaaaaat
     8281 tgagagggat tttcacgtaa catcacatcc acatcggcct gactgaatcc tgactggcgc
     8341 aattgcggaa taaaggtggt tagtaaatag tcatagccat aaccaccgtt ggcttttaaa
     8401 tgggagcggc gcgtaatatc catcgacagc atgacgcggt tcagcaaccc acggtcgcgt
     8461 agcgcatgaa gcatcgcaat acgcttttcg tccgggtagt aactgttctt gccgatggtg
     8521 tcgaactgca cgtacgcgcc gagatcgatc atcttcaaaa tgttgtcgag gttgtctttc
     8581 agatcgcagt gaccaacggt gacgcgcgaa agatcaaccc cgtgggcttg tagcaacgcc
     8641 agttgctcca gccccatcgt gctgaacgac gtatgcgtgg agatcgggcg tccggtctgg
     8701 ttatacgcca gcgcagcggc aataaatacc ttctcttcca gcggcgtaat ctttccttcg
     8761 ctggtgccga tctccgcgat gatcccggct ttcagctccg tgccatcgat accctgttca
     8821 atttcatcga ccatctcctg cgccagttcc tgcacgctgc gggtcgccac atgttccggg
     8881 aaaaacgcgt cctggtaata accggtacag gccaccacgt tgatccccgt ctcgcgcatt
     8941 acatcaagca taaattgcgc attgcgcccc atgtaacggt tggtcatctc aatcacatta
     9001 cgcacgcccc gggtcatcag gtcgttcatc tcctggcaaa tgaacgcata ctgatcaagg
     9061 cggcagtcca cgttgttttt aaagccggag agatcaatat gcagatgctc atgggcgagg
     9121 gtgtaacccg tcggatcaaa actcataggg tgtctcctgc aaaccggagg gaggaaagaa
     9181 aagagcgacc attttgtggc ggtggcgcgc ggaaaaattc acacacggta gcccccacat
     9241 cagaaagcgt ggtgcgcaca ccgagctgcg tagcgatcat cccttgctga taaaccagca
     9301 ctggcaccac ttcgcgggta tggtggctgt gaccaatggt cggatcgttg ccgtgatccg
     9361 ccatcacgac caggcaatca tctggctgca tcgcctcaac aagccgggca aggttacggt
     9421 caacgacctg caaacgttcg gcataacgtg cgacgtcttc tgcatgacca gcgaggtcgg
     9481 tttcctgaat gttggtgcaa ataaacgccg tcggatgggt gttaaattcg ttgagggtga
     9541 tatccataat ccgctggcta tccaccagat tttgccagct cacgccataa ggattgttga
     9601 cgatatctgc caccttaccc accagcacgg ttggcacgcc tgcttcatac agtttttgtg
     9661 gcacctgcac tttttcatcg acgccatagc ccatatgcac gacctggaaa ccgttgtcat
     9721 aagcgccaga acgcggcgca ttgataccaa taaagcgccc ttctttgctt tctgcggcat
     9781 cgagaatgcg ttgactgtcg gttaacaggc caccaaatgt aatgacccga ccgacctgta
     9841 cctgctcacg cacgatacga ccaattttga ttgcgtcgtc aaaagagatc acagagagat
     9901 tggcggtaat gttatagacc tggcctaaat ccgcctcgag attatcgcca atcgcaaccg
     9961 cctgattgac ccacagaaat tgcagatcat cgccacggcg ctccacctgc cagccagcgg
    10021 aaactaatgc ctgctcaaca cggtcaatca catcgcgaaa aggcatccgc agcggcggta
    10081 acgggcgcgt gcctaaaatt tcctgatgcc ccataaaggt atcgccacct tcatgttgca
    10141 gctctgccac gccccaggtt gcggaatctg acggctgcat atcgcctggc gcataaccca
    10201 atgcgttgat tagccccagc ttctccagcg ttggtagctg caaatgcggc aactggctca
    10261 ggatgtgacc acatgtattc gctcccgcat cttgcggacg caccagcgtg acatctttca
    10321 ttgcccctac gccaaagcta tcaatcacta acaccacaaa tcgcgccatt acgcccccag
    10381 agagtttcca agactgtcat aacgaccgac gatttccggt tcgccacgat gaataccgga
    10441 caccagcacc acatcgctac gggtgacaaa aatctgcgtg cgaaaacaga gcaccactgc
    10501 actgcttacc ggaaactcgc ctgccagcgg cagggtgtag tcgatactgc tgtcatccac
    10561 agttttgaga ttggtttcag taatcttttg attttctggc gtaaacacca acgcatgttg
    10621 cgcatgacca cgacgatagt aaccgccgcc gtagcagtag ctgtcgccac ggaaatgatg
    10681 ggagatttca cttaaccaga gcatcgcgat acgttcaggc tgatcgccct gctggtttgc
    10741 cggaatagtg cccgtcagcg catgaccggg ttcggcatga gtcacaccgt attgcgccag
    10801 taatggcagc gaagtgcagc tggtcgctga aggcgcgttc agttgctcaa gtgcaatacc
    10861 agatttcgcc agttgatccc gtgcctgtat cagcgtgtga agattcggtg tcggcaaaac
    10921 ttttccgaca gcctcatccc aaagcaggca agggaaatgg gtaagtccgg ctaaatgcag
    10981 ccctggcaga ttctggattt cggcgacaat ctccggcaac accttgaggg caaaaccgct
    11041 ctcctggccc ggataaagaa aatcatcgtc gctataaact ttaagcagca cagactggat
    11101 tcgcccggcc ttcaccgccg ccgcagaaac ttcccgcgct ttgtcgagag taaacacggt
    11161 gatgacgtcg gtgccctgtt caacggcgtc agcaacctga tgacaaggga tttgtaccag
    11221 atgcccctga tgcgccacag gcaaaccagc gcggcgcatg actcgcgcct ctttgtaatc
    11281 caccgccaca atgccgctgt agcctaatgc cagcaatttt tccgccagcc acggattacg
    11341 accaaactgc ttggtcatca gatacagttc aatgccgtaa agccgcgccg tctcaatcag
    11401 ccgcttaccg ttttccagta tctgatccac gtcgatcacc cagctgtccg gggcgatctt
    11461 gccctgctgc cacaggctta gtgcggcgga aatcagcgcc gggttctggc gtttcaatgc
    11521 ttctacaaac atcttgttgc ctcgtttctg ttccttgaat taaatattca tttttttgaa
    11581 tatttaggtt gtgcgacgtg gatttgtcgg gagcttcaaa cctcttcgtt agcagccatc
    11641 cataatccat aaaggttggc cagcaaatag ccttcttcac acgggttaac ttccagtgcg
    11701 aattccttca acaacacctg atgcaattgc acaacggctt gccagtggct ggattgcgcc
    11761 agttctgcca gcaactcgtt atccagcggc tctatttctt caccacggcg actgcgcatc
    11821 agtgcactcg ccatatgtgt catcgccatc gttccttgct cactgcgcac cggcagatgg
    11881 cactctgttt ccagtacgtt gacgacctgc atcatgcctt tgcagatgtc cttatcaata
    11941 acgcctgcct cgcaaagcag gttgagtctg gtttccatat cagatgcctt tcgtttaata
    12001 actcggttcc tgttccccac tcactacccg ttgttgatgg gcgagcaggg cgtgtttatc
    12061 tacaacggca cgcagcagtt gttgcattgg gtaatcatcg actcgcgtca ggaccacggc
    12121 ttcggtggcc tgtaaaaatc gcggatcgtc tgtgagcggc gtcgcctcta atcccagcat
    12181 ggtcagttcg ttttccgcca ccacgttcca gatcaccgca tcgacatcgc ctttaacaat
    12241 gcgttgtaaa ctctcgtgat aagagagatc gactcgttcc acatcactac cgccaaaaaa
    12301 aacatcggtc atgattttct gatccgccga acggttatcc agccccacgc gcttcacgtt
    12361 tgcggactcg cctttacggc aaatcaactg gtgctcgcca acataggtgt gcggccccaa
    12421 ctccagcgcg aggcataagc ctttttgcgt gagataactt tccgccgcta gtcgcgaaac
    12481 caccgccatg tcatacacgc cattaagcag acactccacg cgaatatccg cgccacgcat
    12541 gtgcgcatag taaaaaggaa tgccatcaaa ctgggctttc aatccgctcg ccaggccttc
    12601 gtacaaacgg gtatagggca agggcattgc acataccacg ttgttgatat ccacatgagt
    12661 cagcaatgct ttgttatcca tctcgaccag ataactgcca ttgcgcccac ggcgttcaat
    12721 ccgtatcgct ccgcttgatt ccagcgtttt caacgcggcc tgcgtcaggc caacggatga
    12781 acggcattca tttgccagct catctatggt tttcaatcga ttaccgcact tttcacccaa
    12841 caaataacgg gccagcgtcg tgatgacgac gccttctttt ttgataaacg ttcgacgcat
    12901 aataaatttt cagtaaattg aatatttata tcttcaggaa tttgaagata aggcgcaaca
    12961 gcgagatgtg gaaacggcga ggcacttcac attttttcgg attattgcaa atgaggataa
    13021 aaaaaccggg tttccccggt ttcagagtga tgataaaagc aaaattgcct gatgcgctac
    13081 gcttatcagg cctacatttc cttgcaatat gtgcattact ttgtaggccg gataaggcgt
    13141 tcacgccgca tccggcatga acaaagcgca atttgccagc aatagtgaat tacggcttcg
    13201 ccacaaaacc aatcgcttcg tacaccgctt ttagcgtacg ggaagcgtgc gcgctggctt
    13261 tttccgcgcc atctttcatc acctgttgca ggaaggcttc atcgttgcgg aaacggtgat
    13321 agcgttcctg caattcagtc agcataccgg aaacggcatc agccacttca cctttcagat
    13381 gaccatacat cttgccttcg aactgttttt ccagttctgg gatgctctgg cccgttaccg
    13441 ctgaaaggat atccaacagg ttggaaacgc ccgctttgtt ctgcacatcg tagcgaacta
    13501 ccggcggctc gtcggagtca gtgaccgcac gtttgatttt cttcactacc gatttcggat
    13561 cttccagcag gccgataacg ttattgcgat tatcgtcaga cttggacatc ttcttggtcg
    13621 gctccagcag cgacattacg cgcgcgccag atttcggaat aaacggctcc ggcaccttaa
    13681 agatctcgcc atacagcgcg ttgaaacgct gggcaatatc gcggctcagt tcgaggtgct
    13741 gtttctggtc ttcacccacc ggtaccagat tagtttgata cagcaggatg tccgctgcca
    13801 tcagcaccgg atagtcaaac agaccagcgt tgatgttctc ggcataacgc gcagatttat
    13861 ctttaaactg cgtcatgcga ctcagttcgc cgaagtaggt atagcagttc agtgcccagc
    13921 ctaactgtgc atgttccggc acgtgggact gaacaaaaat ggtgcttttc tcaggatcga
    13981 taccacaagc cagatacaag gccagcgtat ccagcgtcgc tttacgcagc ttctgtgcat
    14041 cctggcgcac ggtgatcgcg tgttggtcaa cgatacagta aatgcaatgg tagtcatcct
    14101 gcatgtttac ccactgacgc agcgcaccca tgtagttacc aatggtcaat tcacctgagg
    14161 gctgtgcgcc actaaaaacg atgggcttag tcatttttcg attcctgatt ttcgctatgc
    14221 ggaagcccta atgcgggcag aaggtcattt atagactgat aaattacatc aggctggctg
    14281 agatcgatag cctcgccgta gttatatccg taggttaagc caactgatgg gcaacctgcc
    14341 gcttttgctg cctgaatatc attgcgtgag tcgccaacaa acagcatctg ttgtggagca
    14401 attcccatcc gctcagccac cagtaacagc gggtccggat gcggtttttt gttttgcaca
    14461 tcatcaccac caatcaccac gctgaagtat ttggcgatat ctaaggcttc gagcagcggc
    14521 gcgacgaacg gcgtcggttt gttggtgacc aggcctagcg gcaggccttt agcctgcaac
    14581 gcgcccaacg tatcggcaac gtgcgggaac aaaaacgtcc cctcttcggc aacctcgcca
    14641 tagtagcgat cgaacagttt acgcagaata cgtacctgtt cttctgccgg aatgtcgtca
    14701 tcaacgggcg gtttacccat tgttttacgc agagtcgcac gttcctgacg cgcccaggtc
    14761 aatgcgcgct ccatcagaac atctgcgccg ttaccaatcc aggtaataac gcgttcttca
    14821 cctgcgacgg gcaactccag cgcatacagc gccatatcta ccgcagcagc aagaccggga
    14881 gcactgtcca ccagcgtacc gtcgagatca aaagcgacgc cgcgaatatc ttcaaactta
    14941 ctcatgactt acctttgcca gttcactgcg catttcatca atgacttttt tgtagtctgg
    15001 ctggtcgaag attgccgaac cggcgacgaa catatccgcg cccgccgcag cgatttcgcc
    15061 aatgttgttc accttcacgc caccgtccac ttccagtcga atgtcgaagc cagactcgtc
    15121 gatacgacga cgtacttcgc gcagtttatc cagtgtttga ggaatgaaag actgaccgcc
    15181 gaaaccaggg ttgacggaca tcagcaggat cacatccagc ttatccatca cgtaatccag
    15241 atagctcaga ggtgtcgccg ggttaaatac cagacccgct ttacagccat tttctttaat
    15301 cagttgcagc gtgcggtcaa catgctcgga ggcttctgga tgaaaggtaa tgatgctggc
    15361 accagcggca gcgaaatcag gcacaatgcg atcgacaggt ttcaccatca ggtgtacgtc
    15421 gataggggcg gtaatgccat agttacgcaa ggatttcagc accattggcc caatcgtcag
    15481 attgggaaca tagtggttat ccatgacgtc aaaatgcacg acatcagcgc cagctgccag
    15541 ggcttttgcg gtatcttcac ccaggcgggc aaaatcagcc gacagaattg agggggcaat
    15601 caaatactgt ttcatccgct tctccttgag aattattttt tcgcgggtga aacgactcct
    15661 ggtttgtaca aagccagcag ttcgtccacc tttttacgtg tgccgccgtt gctgcttata
    15721 ctgcgtcgaa ctttgacgac atgcaatttt gcgcgctgat accactcacg cgttaacatc
    15781 gtatcgtgat tggagatcag cactggaata tggcgatcaa ccagaccttc ggcgatctcc
    15841 gccagatgcg cttgttgttc aagcgtaaaa ctgtttgtgt gatacgccgt aaagttggcg
    15901 gtcgcagaca gcggtgcata aggcggatcg caatagacga cagatgcatc atctgcgcgc
    15961 gccatgctat cagcgtaaga ctcacaatag aaaaaggcat tctgcgcttt ttcagcgaag
    16021 tgatacaact ctgcttccgg gaaatagggt tttttgtagc ggccaaacgg cacattaaac
    16081 tcaccgcgca gattgtaacg acacaggccg ttgtaaccgt agcggttcaa atataaaaac
    16141 agtaccgccc gacggaacgg atcctggctt ttgttgaact cttcgcggaa ctgatagtaa
    16201 acctcggcgc aatttgtttc gggaacaaac agctcgcgtg cggcctgtac gtactcatca
    16261 gtacgcatct tcacaatgtt atagagactg atcaggtcgc tattgatatc ggcaaggata
    16321 taacgagaaa agtcggtgtt gagaaacacc gacccggcac ctacaaaagg ctcaaccaga
    16381 cattcgccct tgggcaaatg ccgtttaata tcatcaagca ggggatactt gccccctgcc
    16441 cacttcaaaa aagcgcgatt tttcttcatg ctgactaact aattacacct tctccggctg
    16501 tggagaaagc tccgacagca tccagcgctt caggcagctc ccgcaacgat aaaccattgc
    16561 gggagataac cttgattact tcagatcggc ctgtacctga cgcagcggtt tcgcccacgg
    16621 gtttttggcc tgaacatctg ctggcaatgt agataccgct tttttcgcct cttctttcga
    16681 agcgtacacg ccagaaacca ggacatacca cggctgacca ttacgcgtcg tttcatagac
    16741 aacgtagttt ttcagattct ctttcttcgc ccaaccgttc aggttgtcgt agttagagga
    16801 actgctcagc tgcagagtgt aatggctgga cggtgccgat ttcaacgaac caacattacc
    16861 tgcggtcttc cctccagcgg ctggtgttgc cgtggtttgc gccggggatg ccgtctgtac
    16921 tggagccgta gtcgccgtct cttttggtgc tggcgtagaa gtcgcagccg gtgccttcgt
    16981 gctcgccaca ggggcagctg gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    17041 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gagctgggcg
    17101 cgtagtggcc ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    17161 gcgaacaggc gcgacagtcg cgggttcggt cggcaatgtg gaattgaccg ccacattgtt
    17221 caactgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    17281 acgttgttga ccatccgttg ccgccggggt ttgcccttga gtcggcgtag aagagatcgg
    17341 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    17401 cccattcgcc tgatcggtcg cattgcctgc aagatcaata ctcttctcgc cagacgcggt
    17461 ttgatcgctg gaagaggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    17521 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    17581 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    17641 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    17701 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    17761 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    17821 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    17881 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    17941 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    18001 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    18061 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    18121 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    18181 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    18241 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    18301 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    18361 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    18421 acaacgcatc cagattctct tccagccagt tgaaaaacgc accgtcaaga ataatgccgt
    18481 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    18541 cgagatccac caccactgaa gcaggttggt agaacgcgcc aatcatgttt ttaccgaggg
    18601 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aatgtcgtcg
    18661 ggacttgaat gaaacggaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    18721 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggt ttttgtaaca
    18781 acgccgtaaa gacggtatcg agtacagcca ggcttttata ctgctcgccg tcagggagga
    18841 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    18901 gaggagccag ggtttcgttg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    18961 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    19021 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    19081 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    19141 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    19201 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    19261 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    19321 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    19381 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    19441 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    19501 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    19561 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    19621 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    19681 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    19741 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    19801 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    19861 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg cagcgaagag
    19921 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    19981 aacacgttca gcgtaaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    20041 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    20101 attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc gatttatttt
    20161 tacgggtaaa aatgccgccc agcgccaacg tttctccgct tttgacctcg acctgcgttt
    20221 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    20281 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    20341 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    20401 tggaaacctg atatggaatt tcgctcccct gtttaatgct ggcaggctga agatgtgagg
    20461 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    20521 caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    20581 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    20641 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    20701 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    20761 gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    20821 tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct cccgcgtcgg
    20881 cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    20941 ccgcctcctg gcgggcgata ttgttattct gccaggcaat ggaatgcact gagagaatgt
    21001 tgccttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    21061 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac acgaccaggt
    21121 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    21181 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    21241 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    21301 cagtttgtcc cggtacccag cgttagtacg tctggcgtca gctgtaaaat tgtccagccg
    21361 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    21421 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    21481 atccggcata gatcttccgg cggtttaaaa gggtcacgca taccggttaa aaggcacaat
    21541 gcaatacctg ccaacaacca gcgtttaacc ctcatgcggc gtctccagtt gtagcgtgaa
    21601 caaaagatca tcaccttcca cgcttaacga aaaacggctc acgctgacgt tgcgctctgc
    21661 cagccgtgta aatgccgacg gcactgcttc ccacagcgtt ttcaacgcca actcgcctcc
    21721 ctgcgcggat ggatgccagg aaaccagttg cgcgccggat aactgaaaat ccagtggcga
    21781 aaagggcagc gttttttcct cgctgaaggg agtggtgtct accaggcgat acagtgcggc
    21841 ccactgctgg tgatgacttg cccgtagtcg aattaatgcg tcgcgctctt catggtgtgt
    21901 cgatgacaga aaaatgagcg taactaacat cagcaaccag aatgcccagc aaagctggcg
    21961 gaggcggggt gatgtggcga accaccagtc aaagaacatg ttcatcgcta accttccttg
    22021 ttaactgata ctcaaattgc cagcgtccct gcgcatcctg ctgcgtggct ccccgctgat
    22081 tgagatgaaa agatgcatcc tggcggagtg acgtttctag tgcgtttaac gcggtaatgc
    22141 ttgttgtcag ccccttgatc tccagcgttc cctgctgcca gcttatcgtt gtcagccagg
    22201 cgtgctcggg taaaagcgcc gccagtgctt ccagcgcaga ttgccagtcg cgggtaaatt
    22261 gtcgctggcg ctgccgttgc gaacgctgtt cgcgtaactg ctgttgctcc agcaaacgtg
    22321 gcttcgttat ctgtaagctg cgggcgagtt gttgttccgc ctgaagcaaa actgcgtcta
    22381 tgcgcgcttc ggcgctgcct gtcagacgca gtattagcgt tatcccgacg gccagcagca
    22441 gaggcgcaac gaacatcagc aaccagaaac gcagaaaagc ggtccggcgt tgctgtcgcc
    22501 agggcaaaaa attaattggc gggttcatca gtacgccttc ccaagcgcca gccccagcgc
    22561 gatggcaaag tctccacctg gcggcggtag cggcggctga cgaacagaaa cggcctccca
    22621 gggatcaaat ccgccttcgc cacatatcgc gacgctttcg ggatcaacgg ataacgctgc
    22681 cgccagctcc ttcgcgctag tcatccccac cgccagtttg cgcccccagc gacagcgtgt
    22741 cgcccacagc cactgttcgt tatcacgcca ggccagacat tgctgatgag aaggtaaaaa
    22801 aggcaggaat tgctgtaatg cactggcatc cggggtgatc gcgctcacat gaacacgcaa
    22861 cctttctgcc agagtaagta gcgttgccag ctctttgctt tgcgcggcag tcacgttata
    22921 ggcggggctg agtgagtctt cgctgtaatc gaagcgcagg gagtccggat ccatatccag
    22981 ctcgcgggcc atcgtccctg acagccaggc cgtttgctcc cgctcaccaa gggacatcga
    23041 cgggcgcgga aatgaccgct gtaatgtgcg actggcggga aacgccaaca taatgtgatg
    23101 acgctgcggc agttcgcgac tccacggtaa caacgtttta gccagctgct gcgcatcaac
    23161 aatccgccca tctttgataa tgtcgttctc cagcggcaac cgccaccagc gttgcaaaaa
    23221 gcattctttt gctccccgca cgatcgcaac cgctaccgct tcttgctgtt gtaaatgcaa
    23281 accaatttgc cagatcttaa atgccattgt gatgatctcc ttatcacccg tcactctgac
    23341 gggtatatca atgcgtctgg cttgccttta tactaccgcg cgtttgttta taaactgccc
    23401 aaatgaaact aaatgggaaa tttccagtga agttcgtaaa gtattttttg atccttgcag
    23461 tctgttgcat tctgctggga gcaggctcga tttatggcct ataccgctac atcgagccac
    23521 aactgccgga tgtggcgaca ttaaaagatg ttcgcctgca aattccgatg cagatttaca
    23581 gcgccgatgg cgagctgatt gctcaatacg gtgagaaacg tcgtattccg gttacgttgg
    23641 atcaaatccc accggagatg gtgaaagcct ttatcgcgac agaagacagc cgcttctacg
    23701 agcatcacgg cgttgacccg gtggggatct tccgtgcagc aagcgtggcg ctgttctccg
    23761 gtcacgcgtc acaaggggca agtaccatta cccagcagct ggcgagaaac ttcttcctca
    23821 gtccagaacg cacgctgatg cgtaagatta aggaagtctt cctcgcgatt cgcattgaac
    23881 agctgctgac gaaagacgag atcctcgagc tttatctgaa caagatttac cttggttacc
    23941 gcgcctatgg tgtcggtgct gcggcacaag tctatttcgg aaaaacggtc gaccaactga
    24001 cgctgaacga aatggcggtg atagccgggc tgccgaaagc gccttccacc ttcaacccgc
    24061 tctactcgat ggatcgtgcc gtcgcgcggc gtaacgtcgt gctgtcgcgg atgctggatg
    24121 aagggtatat cacccaacaa cagttcgatc agacacgcac tgaggcgatt aacgctaact
    24181 atcacgcgcc ggagattgct ttctctgcgc cgtacctgag cgaaatggtg cgccaggaga
    24241 tgtataaccg ttatggcgaa agtgcctatg aagacggtta tcgcatttac accaccatca
    24301 cccgcaaagt gcagcaggcc gcgcagcagg cggtacgtaa taacgtgctg gactacgaca
    24361 tgcgccacgg ctatcgcggc ccggcaaatg tgctgtggaa agtgggcgag tcggcgtggg
    24421 ataacaacaa gattaccgat acgctgaagg cgctgccgac ctatggtccg ctgctgcctg
    24481 ccgcagtcac cagcgccaat cctcaggaag cgacggcaat gctggcggac gggtcgactg
    24541 tcgcattgag tatggacggc gttcgctggg cgcgtcctta ccgttcggat actcagcagg
    24601 gaccgacacc gcgtaaagtg accgatgttc tgcaaacggg acatcaaatc tgggttcgtc
    24661 aggtcggcga tgcatggtgg ctggcacaag tgccggaagt gaactcggcg ctggtgtcga
    24721 tcaatccgca aaatggtgcc gttatggcgc tggtcggtgg ctttgatttc aatcagagca
    24781 agtttaaccg cgccacccag gcactgcgtc aggtgggttc caacatcaaa ccgttcctct
    24841 acaccgcggc gatggataaa ggtctgacgc tggcaagtat gttgaacgat gtgccaattt
    24901 cccgctggga tgcaggtgcc ggttctgact ggcagccgaa gaactcacca ccgcagtatg
    24961 ccggtccaat tcgcttacgt caggggctgg gtcagtcgaa aaacgtggtg atggtacgcg
    25021 caatgcgggc gatgggcgtc gactacgctg cagaatatct gcaacgcttc ggcttcccgg
    25081 cgcaaaacat tgtccacacc gaatcgctgg cgctgggttc agcgtccttc accccaatgc
    25141 aggtggcgcg cggctacgcg gtcatggcga acggcggctt cctagtggac ccgtggttta
    25201 tcagcaaaat tgaaaacgat cagggcggcg tgattttcga agcgaaaccg aaagtagcct
    25261 gcccggaatg cgatattcca gtgatttacg gtgatacgca gaaatcgaac gtgctggaaa
    25321 ataacgatgt tgaagatgtc gctatctccc gcgagcagca gaatgtttct gtaccaatgc
    25381 cgcagctgga gcaggcaaat caggcgttag tggcgaagac tggcgcgcag gagtacgcac
    25441 cgcacgtcat caacactccg ctggcattcc tgattaagag tgctttgaac accaatatct
    25501 ttggtgaacc aggctggcag ggtaccggct ggcgtgcagg tcgtgatttg cagcgtcgcg
    25561 atatcggcgg gaaaaccggg accactaaca gttcgaaaga tgcgtggttc tcgggttacg
    25621 gtccgggcgt tgtgacctcg gtctggattg gctttgatga tcaccgtcgt aatctcggtc
    25681 atacaacggc ttccggagcg attaaagatc agatctcagg ttacgaaggc ggtgccaaga
    25741 gtgcccagcc tgcatgggac gcttatatga aagccgttct tgaaggtgtg ccggagcagc
    25801 cgctaacgcc gccaccgggt attgtgacgg tgaatatcga tcgcagcacc gggcagttag
    25861 ctaatggtgg caacagccgc gaagagtatt tcatcgaagg tacgcagccg acacaacaag
    25921 cggtgcacga ggtgggtacg accattatcg ataatggcga agcacaggaa ttgttctgat
    25981 taaaaaggcg cttcggcgcc tttttcagtt tgctgacaaa gtgcactttg tttataccgg
    26041 atacggcgtg aacgcgttat ccggctaaca aaatcgtgaa aactcaataa attgcagaaa
    26101 ccccataagc ctgataaaca ttgtgcatca ggcaaacttc acgcatttac actcgcccct
    26161 gccctttcaa ccattcgcgc acgaggaaca gcgcactgac attgcgcgct tcattgaagt
    26221 cagggtcttc cagcaaatcc atcatatgcg ccagcggcca gcgcacctgt ggtagcggct
    26281 ctggctcatc gccttccagt gattccgggt agagatcttg cgctaccacg atattcattt
    26341 tgctggaaaa gtaagacggt gccatgctga gcttcttcaa aaaagtcaga tcgttcgctc
    26401 caaatccaac ctcttctttt agctcgcggt tagcggcttc gtagacgctt tcacccggat
    26461 caattaatcc tttcgaaaaa cctaattcgt aggattcagt tcccactgcg tattcgcgga
    26521 tcaggatcag gtgatcgtcc acaatcggca caatcatcac tgcttcccgg ttggttggac
    26581 gcattcgttc ataaacacgc cgcacgccat tgctgaactc cagatccacg ctttcgacgg
    26641 taaacagtcg ggaacgggct acagtttcaa cattcagaat ggtgggtttt tgtaatgatt
    26701 tgctcatcgt gggatctatg ctgtgaaatc agcggttatt gtgcgatatc ggacacgctt
    26761 tcggcaatgt gaattgcatg ttatttacat ttatgtaact taataaataa tcgtcctcaa
    26821 atcaaattga aagtcaatag gttgaaataa ctccaggaat ttgctgatat tccgccttca
    26881 gagggtttgc tatgatcagc ggttactgtg atgtgcttaa tgatgctcaa gttaaactcc
    26941 acgcttgccg atagccaacc gcagaatcat gtattgtgtc cggtgcgact gaccacgcct
    27001 gacagactaa gtaagatggg gaaagcatga gcaccattgt gattttttta gctgctttgc
    27061 tggcctgctc actacttgcg ggatggctga taaaagtgcg atccagacgg cgtcagctgc
    27121 cctggaccaa cgccttcgcg gatgcgcaaa cgcgtaaact cacacctgaa gaacgtagcg
    27181 ccgttgaaaa ttatcttgag agcctgacgc aggtattaca ggtgcctggc ccaacgggag
    27241 ccagcgcggc accgatctct ctggcgctga atgccgaaag caacaacgtc atgatgctga
    27301 cacacgctat cacacgttac ggcatctcta ccgacgatcc gaataaatgg cgttactacc
    27361 tcgattcggt agaagtccac ctgccccctt tctgggaaca gtacatcaac gatgagaata
    27421 ccgttgaact gattcatacc gattcgctgc cgctggttat ttcactcaac ggtcatacgc
    27481 tgcaggagta catgcaggaa actcgcggct atgccttgca acctgttccg tcaacgcagg
    27541 cgtcgattcg cggggaagag agtgagcaaa tcgagctact caatattcgc aaagaaacgc
    27601 atgaagaata tgcgctgagt cgtccgcgcg ggctgcgtga agcgttgctg atcgtcgcct
    27661 ccttcctgat gttctttttc tgcctgatta ccccggatgt atttgttccg tggctggcag
    27721 gcggcgcgtt actgctgctg ggcgcaggtc tgtgggggct attcgcgccc ccggcaaaat
    27781 cctccctgcg ggaaattcat tgtctgcgcg gtacaccccg tcgttgggga ttgtttggcg
    27841 aaaacgatca ggaacagatc aacaatattt cactcggtat tatcgacctg gtctatcccg
    27901 cacactggca gccatacatt gctcaggatc tcggtcaaca aaccgatatc gatatctatc
    27961 tcgaccgcca tgtagtgcgt cagggacgat atctttcgct gcatgatgaa gtaaaaaact
    28021 ttccgttaca gcactggctg cgcagtacga ttatcgctgc gggctcgctg ctggtgctgt
    28081 ttatgctgtt attctggatc ccgctggata tgccgctgaa attcactctc tcatggatga
    28141 aaggcgcgca gaccattgaa gccaccagcg taaaacaact ggctgacgct ggcgtgcggg
    28201 taggcgatac attgcgtatt agcggtacgg gaatgtgtaa tattcgaact tccggaacct
    28261 ggagcgcgaa aaccaattca ccttttttac cgtttgactg ctcgcagatc atctggaacg
    28321 acgcccgctc attgccatta ccagaatctg aactggtcaa caaagcgacg gcattgactg
    28381 aagcagttaa tcgccagctg caccctaaac cggaagatga atctcgcgtc agtgcctcat
    28441 tacgttcagc aattcaaaaa tccggcatgg tattgcttga tgattttggc gacattgtac
    28501 tgaagacagc ggatttatgt tctgccaaag atgactgtgt gcgactgaaa aatgcgctgg
    28561 tcaatctcgg caacagtaaa gactgggacg cgctggtaaa acgcgccaac gccgggaagc
    28621 tcgatggcgt gaatgtgtta ttacgcccgg tgagtgcgga atcgctggat aacctggtgg
    28681 caacctccac cgcgccgttt atcacgcatg aaacggcgcg agcggcacaa tcactaaaca
    28741 gtccggcccc cggcggattc ctgattgtca gcgacgaagg cagcgatttt gttgatcagc
    28801 cctggccttc ggcatcactt tacgactacc cgccgcaaga acagtggaac gctttccaga
    28861 aactggcaca aatgctgatg catacgccgt ttaacgccga gggtatcgtc acaaaaatct
    28921 tcactgacgc caatggtacg cagcatattg gccttcatcc gatcccggat cgttccggcc
    28981 tgtggcgcta tctcagcacc acattgctgc tactgacgat gctgggtagc gccatttaca
    29041 atggcgtaca ggcctggcgt cgttaccagc gtcatcgcac tcgcatgatg gagattcagg
    29101 cctattatga aagctgcctg aacccgcaac tgatcacccc ttcagaaagc cttatcgaat
    29161 aacacgtttg cgcggcaggt tatgctaccc tgtcgcgcaa attgcttcac tctggagatt
    29221 tccctcatgc atatcaacat tgcctggcag gacgtagata ccgttctgct ggatatggac
    29281 ggcacgttgc tcgacctcgc cttcgataac tatttctggc aaaagctggt gcctgaaaca
    29341 tggggcgcga aaaacggggt tacgccacag gaagcgatgg aatatatgcg ccagcaatat
    29401 cacgacgtac agcatacgct aaactggtac tgtcttgatt actggagtga gcaactgggt
    29461 ctggatatct gtgcgatgac caccgagatg ggaccgcgtg ccgtactgcg tgaagatacc
    29521 attccgtttc ttgaggcact gaaagccagc ggtaagcagc gaattttgct caccaatgcg
    29581 catccgcaca acctggcggt aaaacttgag cataccggtc tggacgcaca ccttgattta
    29641 ttactttcca cccacacatt tggttatccg aaagaggatc agcggttatg gcatgcggtg
    29701 gccgaagcta cgggtctgaa agctgaaaga acgctgttta ttgatgacag cgaagcgatt
    29761 ctcgatgctg ccgcgcaatt tggtattcgt tactgcctcg gcgtgactaa tcctgattcc
    29821 gggattgccg agaaacagta tcaacgccat ccgtcactga atgactaccg ccgcctgatc
    29881 ccctcgctaa tgtgaaggag acgccatgaa agagaaacct gctgttgagg ttcgactgga
    29941 taaatggcta tgggctgccc gtttttataa aacccgcgcg ctggcccgtg aaatgattga
    30001 aggcggtaag gtgcattaca acgggcagcg cagcaagccg agcaaaatcg tcgagctgaa
    30061 tgccacgctc actctgcgcc agggaaatga cgaacgcacg gtgattgtaa aggcgattac
    30121 tgaacagcgt cgccccgcca gcgaggcagc cttgctgtat gaagagactg cggaaagtgt
    30181 agagaaacgc gaaaaaatgg cgctggcacg taaacttaat gccttaacca tgccgcaccc
    30241 ggaccgacgc ccggacaaaa aagagcgccg cgacctgtta cgatttaaac acggcgacag
    30301 tgaataactg tcacctgcaa gagagatgat tatgccgcaa catgaccaat tacatcgcta
    30361 tctgtttgaa aactttgccg ttcgcggcga actggtaacc gtttcggaaa ccctgcaaca
    30421 gatccttgag aaccacgatt atccgcagcc cgttaaaaac gtgctggcag aactgctggt
    30481 tgcgaccagc ctgttaaccg ctacgctgaa gtttgatggt gatatcaccg tacagctgca
    30541 gggcgacggt ccgatgaatc tggcggttat taacggtaac aataaccagc aaatgcgcgg
    30601 tgtggcgcgc gtgcagggcg aaattccaga aaatgccgac ctgaaaacgc tggtcggcaa
    30661 tggttacgtg gtgatcacca ttaccccgag cgaaggcgaa cgctatcagg gcgtagttgg
    30721 tctggaaggt gataccctgg cggcctgcct ggaagattac tttatgcgtt ctgaacagct
    30781 gccgacgcgc ctgtttattc gcaccggcga cgtagacggc aaaccggctg caggcggtat
    30841 gttgttgcag gtaatgcctg cgcaaaatgc ccagcaggac gactttgacc acctggcgac
    30901 gctaaccgaa accatcaaaa ccgaagaact gctgacctta ccggcaaacg aagtgttgtg
    30961 gcgtttgtat cacgaagaag aggtgacggt ttacgatccg caggatgtgg agttcaaatg
    31021 cacctgctcg cgtgaacgtt gcgccgatgc gctgaaaacg ctgcctgatg aagaagttga
    31081 tagcatcctg gcggaagatg gcgaaattga catgcattgt gattactgcg gtaaccacta
    31141 tctgttcaat gcgatggata ttgctgaaat ccgcaacaac gcgtctccgg cagatccgca
    31201 agttcattaa tgagtatgtc cggcagagag ggctctgccg gattttacga ctaatacacc
    31261 tgatgcgctt tgtcactcat tcagattcac attcagcctt tcaccatcca ctacaagatc
    31321 gcgccacgct ttaggcttcg ttcccagttt tccatccttc gcagctgtga gtaatttttc
    31381 tttcgttata tcacgcggaa gtaaactcat cgtaagctct tgccattctt tcttgtcagg
    31441 atcaaagcca tagacaatgt atctttcatc atcaaaggca aagaggatcc gctctgccct
    31501 gccatcacta ttcagatctt gctcgaccag tacgcaggcg tctttttcaa tacaggtcat
    31561 cacgttatag cgttctttga ttaatgccga ccagaatgcc gcatccggtt tgccagaacc
    31621 tggtgcaatt aacacatttt cggctaatga tttttccgac accaccttct gaagatgttg
    31681 ctctccatct aacgccatca gcagatcccg cgcgcgtatc gggtctttca tatactcggc
    31741 attgcttttc agcgactcaa gcgccgcacg tccatagcga ccgctctgtt cgagcatgta
    31801 gatagttacc tggtctggcg tgtttttgcc gctctgataa cgcgccatat gactgttcac
    31861 gctaatgcgc atactgtcca gcaccggcga attaagaagc accagtatca ccaacaccaa
    31921 taacgaaacc gcaaggttca ctttgccctg aagaacaagg ggattttgcc cattacgcca
    31981 gacgatactg acaaaatacc ccagcgacca caccagtaac accagcaccg ccagggcgcc
    32041 ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc agccataacg cccaggcggc
    32101 gacgaacaca tacagcggag caaccagcaa agcggtttta atcaggcaac gcaacggccc
    32161 cgtccaggga agtgacgctt tttgcggatc gcggacaata gccattaaga tcaattgcaa
    32221 aaaggccagc gttaacagca acccggcggc ggagatgtgg cgagaaatcg cgctcaggcc
    32281 cgtaaacggc agggtgatga taaacatcag ggttagcaat gatactaacg gcagcaaccc
    32341 cgtggcgatt aacgtgaaca acttttgaat agagtcgatt aaacgtgact gtgttcgcgc
    32401 gaggatcacc gccagcgcgg taaccagacc taacgtgaga taaataaacc agttggttgc
    32461 aaaaaagagc gtattgaaaa acgtgatgcc aacaagttta aacaactcac tccacagtaa
    32521 aagcaccagc cacgtcaagc cattggcgag aaaaatcacc agtaatataa gtacgttatg
    32581 ccatactgac tggtaaaaat agcgatagcg gctactgtca ttgcggatgc gaaggctttg
    32641 ttgtatccac ggtagcaaca acattgccat cagcagcaga tagcaaccaa aatcccaaag
    32701 cgccttttca gctctccagg ggttcatgcc atcagtttgc cacttcagcc agccgctcat
    32761 ccccagcgtg gcaataaaca ctagcgccag ccatccccaa aggcgcttct gtttaaaaga
    32821 gatcacggag aagagtaaaa cggatgaaaa cgcgacggtc gcaggcacgc cataaacaat
    32881 ccaactgtga ttttttccgg aaagccaggc gatcagcagg tagcaaacca gcccctgaag
    32941 taatccagta gcaatcatcc cccagcgtgt tgcaggtgaa agttcaacgt tatccatgtt
    33001 ggttatccag aatcaaaagg tgggttaatt atcgcatccg ggcagtagta ttttgctttt
    33061 ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat actttaccgg ttgaatttgc
    33121 atcaatttca ttcaggaatg cgattccact cacaatattc ccgccatata aaccaagatt
    33181 taaccttttg agaacatttt ccacacctaa aatgctattt ctgcgataat agcaaccgtt
    33241 tcgtgacagg aatcacggag ttttttgtca aatatgaatt tctccagata cgtaaatcta
    33301 tgagccttgt cgcggttaac acccccaaaa agactttact attcaggcaa tacatattgg
    33361 ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc cgcaagaact cgaggcttat
    33421 ggtatcagtg acgtacatga tatcgtttac aacccaagct acgacctgct gtatcaggaa
    33481 gagctcgatc cgagcctgac aggttatgag cgcggggtgt taactaatct gggtgccgtt
    33541 gccgtcgata ccgggatctt caccggtcgt tcaccaaaag ataagtatat cgtccgtgac
    33601 gataccactc gcgatacttt ctggtgggca gacaaaggca aaggtaagaa cgacaacaaa
    33661 cctctctctc cggaaacctg gcaacatctg aaaggcctgg tgaccaaaca gctttccggc
    33721 aaacgtctgt tcgttgtcga cgccttctgt ggtgcgaacc cggatactcg tctttccgtc
    33781 cgtttcatca ccgaagtggc ctggcaggcg cattttgtca aaaacatgtt tattcgcccg
    33841 agcgatgaag agctggcagg tttcaaacca gactttatcg ttatgaacgg cgcgaagtgc
    33901 actaacccgc agtggaaaga acagggtctg aattccgaaa acttcgtggc gtttaacctg
    33961 accgagcgca tgcagctgat tggcggcacc tggtacggcg gcgaaatgaa gaaagggatg
    34021 ttctcgatga tgaactacct gctgccgctg aaaggtatcg cttctatgca ctgctccgcc
    34081 aacgttggcg agaaaggcga tgttgcggtg ttcttcggcc tttccggcac cggtaaaacc
    34141 accctttcca ccgacccgaa acgtcgcctg attggcgatg acgaacacgg ctgggacgat
    34201 gacggcgtgt ttaacttcga aggcggctgc tacgcaaaaa ctatcaagct gtcgaaagaa
    34261 gcggaacctg aaatctacaa cgctatccgt cgtgatgcgt tgctggaaaa cgtcaccgtg
    34321 cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa ccgagaacac ccgcgtttct
    34381 tatccgatct atcacatcga taacattgtt aagccggttt ccaaagcggg ccacgcgact
    34441 aaggttatct tcctgactgc tgatgctttc ggcgtgttgc cgccggtttc tcgcctgact
    34501 gccgatcaaa cccagtatca cttcctctct ggcttcaccg ccaaactggc cggtactgag
    34561 cgtggcatca ccgaaccgac gccaaccttc tccgcttgct tcggcgcggc gttcctgtcg
    34621 ctgcacccaa ctcagtacgc agaagtgctg gtaaaacgta tgcaagcggc aggtgcgcag
    34681 gcgtatctgg ttaacaccgg ctggaacggc actggaaaac gtatctcgat taaagatacc
    34741 cgcgctatta tcgacgccat cctcaacggt tcgctggata acgcagaaac tttcactctg
    34801 ccgatgttta acctggcgat cccaaccgaa ctgccgggcg tagacacgaa gattctcgat
    34861 ccgcgtaaca cctacgcttc cccggaacag tggcaggaaa aagccgaaac cctggcgaaa
    34921 ctgtttatcg acaacttcga taaatacacc gacacccctg cgggtgccgc gctggtagcg
    34981 gctggtccga agctgtaatg atttgaagct ggagaatatc tatccagtat cttatagaaa
    35041 gcaaaacggg aggcaccttc gcctcccgtt tatttaccct tcttttgtcg tgccctgcgc
    35101 ccgcgttacc ggcactggca gccaggcgcg aatggaaagc ccgccccgct cgctggtgcc
    35161 aagctccagc atcccgttat ggttatccac gatacgctgc acaattgcca gccctaatcc
    35221 cgtgccgcta atggtgcgcg cactgtcgcc gcggacaaac ggctggaaca ggtgcttacg
    35281 ttgttccggc gcaattcccg gaccgtcatc ttccacctgg aaccaggcgc gattcggctc
    35341 cgtcccgctg ctgactttga tccagccatt gccgtaacgg gcggcgttga ccaccatatt
    35401 cgccaccgcg cgtttgatcg acagcgggtg cattttcact tcaatgctgc cggggtaaag
    35461 cgcggtttca atttcccgct catagccact ttcggcagca ataacctcac cgagtactgc
    35521 gttgagatcc gccatttcca tcggcatctc ctgtccggtg cgcaggtagt cgataaactg
    35581 ctcaatgatg gcattgcact cctcgatatc tttattgatc gattctgcca ggtagccatc
    35641 ctgctcgctc atcatctcgg tcgccaggcg aatacgcgtc agcggtgtac gcaagtcgtg
    35701 acttaccccc gccatcagca gcgtgcggtc atccgccagt tgcttaacac cagccgccat
    35761 atggttaaag gcgcgggtaa cggaacgcac ctcggacgcg ccatactcac gcagcggcgg
    35821 cggaataatc cctttaccga cctgcaaagc agcatgttcg agatcgacca acggtcggtt
    35881 ctggatacga ataaacagcc acgccccgcc tatcgccaat agcataatcg ccaacgtata
    35941 gcggaacagc ggagaaaaat cgccctgatg aatttcggtc agcggcacgc gtacccagat
    36001 attgggcgac agccaggttt tcagccagac gacaggcgaa cttttgttga cctcaacgcg
    36061 cacctccgtc gggccgccca gttgctgcgc catctgatgg cttaagaatt catagtgttg
    36121 tgcccaacgc agacctgcct cttcggcagc ttcgttggag tagagagaga tccccagctc
    36181 acggtagatc tcccgacgga aagcgggagg cacaaccaac tgcgtgccgt cctccagttg
    36241 cagtttgtcg gtcatcaaca tacgcacttc gtacgcgagg actttattaa actgctggag
    36301 gctcggcaaa atcgcgaagt tcagcaccac cagataagtc gtcaccaggc tggcgaacag
    36361 caaggtgacg atgagcaata acgtacgggc aaatgaactt cgtggcgaga agcgcaatcg
    36421 cctcatgctt tagagccgtc cggtacaaag acgtagccca gaccccagac ggtctgaatg
    36481 taacgcggat gcgctggatc ttcttccacc atgcggcgca gacgcgaaat ctgcacgtcg
    36541 atggagcgtt ccattgcgga atattcacga ccacgggcaa ggttcatcag cttatcgcgg
    36601 gagagcggct cacgcggatg gctgaccagt gccttcagta ccgcaaactc accgctggtg
    36661 agcggcatcg gctcgtcttc gcggaacatt tcgcgcgtac cgaggttaag tttgaactta
    36721 ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg gcagttcgtt cgcctgacga
    36781 cgcagcaccg cacggatacg ggccagcagt tcacgcgggt taaacggttt tggaatgtag
    36841 tcgtcagcgc caatctccag gcctacgata cggtccactt cttccccttt cgccgtcacc
    36901 ataatgatcg gcatcgggtt gctctgacta cgaagacgtc ggcaaatcga caagccatct
    36961 tcaccaggta acattaaatc cagtaccata agatggaaag attcacgagt cagcaggcga
    37021 tccatctgtt ctgcattagc gacgcttcga acctggaagc cttgttcggt gagataacgt
    37081 tccagcagcg cacgcaggcg catgtcgtca tcgaccacca gaatcttgta gttctcttgc
    37141 attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg tgtattctta aaaaagctca
    37201 cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa attgttacaa agcatattaa
    37261 acagcagctt aagtatacaa tttattcggc gaaacattat tgattctgtt gatatgatca
    37321 cgttataccc aatgtgcgca ttatcaaaca gacaaaggga atcaacgaga tgaaaacgcc
    37381 cttggttacc cgggaagggt atgaaaaact caaacaagag cttaattatc tctggcgtga
    37441 agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca agtctgggcg accgcagcga
    37501 aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa atcgaccgtc gcgtgcgcta
    37561 tctcactaaa tgcctggaaa atctcaaaat cgtcgattac tcccctcagc aggaaggcaa
    37621 agtctttttt ggcgcgtggg tggagattga aaacgacgat ggcgtgactc accgtttccg
    37681 tattgtcggc tacgatgaaa tttttggccg taaagattac atctctatcg attccccgat
    37741 ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggcg gtggtgaata cccctgccgg
    37801 ggaagcgagc tggtatgtta atgctatcga gtacgtgaaa ccgtaaggaa gagtcttaac
    37861 ctcctgccga tggctggcat ttttgccagc cagtccgtat aactatcccc tgatttttga
    37921 tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc tgccgcatta ttgcgggtga
    37981 aattcaggcg cgcccggaac aggttgacac tgccgttcgc ctgcttgacg aagggaatac
    38041 cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc ggtctggatg acacgcagct
    38101 gcgtaatctg gaaacgcgtc tgagctatct gcgcgagctg gaagagagac gtcaggcgat
    38161 cctcaagtcc atttccgagc aaggcaaact caccgatgat ctggcgaagg ccatcaacgc
    38221 caccctaagc aaaaccgaac tcgaagacct ctacctgccc tacaaaccta aacgccgcac
    38281 ccgcgggcaa atcgccattg aagcagggct tgagccgttg gctgacctgc tgtggagcga
    38341 tccgtcacac acgccagaag tcgccgctgc acaatatgtt gatgccgata aaggcgtggc
    38401 agataccaaa gccgcgctgg atggcgcgcg ctatatcctg atggaacggt ttgccgaaga
    38461 tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag aacgcgcatt tggtttctac
    38521 ggtggtgagc ggtaaagaag aggaaggggc gaaattccgc gactatttcg atcatcacga
    38581 accgttgtcc acggtgcctt ctcaccgcgc gctggcgatg ttccgtgggc gtaacgaagg
    38641 cgtactccag ctttcgctga atgccgatcc gcagttcgat gagccgccca aagagagcta
    38701 ttgcgagcaa atcatcatgg atcaccttgg cctgcgcctg aacaatgccc cggcggatag
    38761 ctggcgcaaa ggcgtagtga gctggacctg gcgcatcaag gtgctgatgc atctggaaac
    38821 cgaactgatg ggtaccgtgc gcgaacgcgc ggaagatgaa gcaatcaacg tctttgcccg
    38881 taacctgcac gatctgctga tggcggcccc tgccggactg cgtgcaacga tgggcctcga
    38941 tccgggtctg cgtactgggg taaaagtggc ggtggtcgat gccactggca aactggtagc
    39001 gaccgatacc atttacccgc acaccggaca agccgcaaaa gcagcgatga ccgttgctgc
    39061 cttgtgtgaa aaacataacg ttgaactggt ggcgatcggc aacggtacag cttcccgcga
    39121 aactgaacgt ttctatctcg acgtgcagaa gcagttcccg aaagtgaccg cacagaaagt
    39181 gatcgtcagc gaagctggcg cgtcggttta ctcagcttcc gagctggcag cgcaggagtt
    39241 cccggatctc gacgtttcgc tgcgtggcgc ggtgtctatc gcccgccgtt tgcaggatcc
    39301 gctggcggag ctggtgaaaa tcgatccgaa atctatcggc gtaggtcagt atcagcatga
    39361 cgtcagccag acgcaactgg cccgcaaact ggacgcagta gtagaagact gcgtaaacgc
    39421 cgttggcgtc gatctcaaca ctgcttctgt tccgctgtta acccgcgtgg cgggcctgac
    39481 gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag aacggccagt tccagaaccg
    39541 tcagcaactg ctgaaagtca gccgtctggg gccgaaagcc ttcgagcagt gcgcgggctt
    39601 cttgcgcatt aaccacggtg ataacccgct ggatgcttct accgtccacc cggaagccta
    39661 tccggtggtg gaacgcattc tggcagcaac acagcaggca ctgaaagatc tgatgggtaa
    39721 cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact gatgaaaaat tcggtgtgcc
    39781 gacagtaact gacatcatca aagagctgga aaaaccgggt cgcgatccgc gtccggaatt
    39841 taaaaccgct cagtttgccg atggcgtcga gacaatgaac gacctgcaac cgggtatgat
    39901 cctcgaaggt gcggtgacca acgtcaccaa ctttggcgcg tttgtcgata tcggcgtgca
    39961 tcaggacggc ctggttcaca tctcttcatt gtcgaacaag tttgtggaag atccgcatac
    40021 cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg gaagtggatc ttcagcgtaa
    40081 acgtatcgcc ctgactatgc gtctggacga gcagcctggc gaaaccaacg cccgtcgcgg
    40141 cggcggtaat gaacgcccgc aaaacaaccg cccggcagcc aaaccacgcg gtcgtgaagc
    40201 gcagcctgcc ggtaatagcg cgatgatgga tgcgctggcg gcggcaatgg gcaaaaaacg
    40261 ttaaacgccc ggactggccc ctaaacctgc gcgataaggc gttcacgccg catctggcaa
    40321 cgaacgcctt gcctgacata aaagtgccgg agaatatctc cggccttttt attccacaac
    40381 caaactcata atatattccg gcaatattta tcacttcatt aacaactgaa accttaatta
    40441 aacattagcc agtccgggta attcactatt cgaattatat tttcgctgcg atataacctt
    40501 gagccacatc aacattgagt cagattatta ttcaaaccaa cattcgcaca cattttaagt
    40561 attgctgata gaaaccattc tcattatcat tgtgttgttg attatttaat ctctcctttg
    40621 ttggcaaatc atctggtctc atgtcgctgt caaacgcccc atgaggtagt tatccagtta
    40681 atgagaaaca agtaggcacc tatgcaatac actccagata ctgcgtggaa aatcactggc
    40741 ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc tttctcttgg catgttacct
    40801 ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg accccattca tatcgaaacc
    40861 cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct tattagaagt ggaagcggtt
    40921 tcctgttaat acggtgataa caacaatgaa aaaattaacc ataggcttaa ttggtaatcc
    40981 aaattctggc aagacaacct tatttaacca gctcactggc gcacgtcagc gtgtaggtaa
    41041 ctgggctggc gttaccgtcg aacgtaaaga agggcaattc tccaccaccg atcatcaggt
    41101 cacgctggtg gacctgcccg gcacctattc tctgaccacc atctcatcgc agacctcgct
    41161 cgatgagcaa atcgcctgtc actacatttt gagtggcgac gccgacctgc tgattaacgt
    41221 ggtggatgcg tctaaccttg agcgtaacct gtacctgacg ctacaactgc tggaactcgg
    41281 cattccctgc attgtggcac tgaacatgct cgacattgcc gagaagcaaa atattcgtat
    41341 tgaaattgat gctctgtcgg cgcgtctggg ctgtccggtg atcccgctgg tttcaacccg
    41401 tggtcgcggt attgaagcgc tcaagctggc gattgatcgc tataaagcta acgagaatgt
    41461 ggaactggtg cattacgcac agccgctgct caacgaagca gattcactgg caaaagtgat
    41521 gccttccgac atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc tggaaggcga
    41581 tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat ctggatgccg ccctcgcccg
    41641 tctgcgtaat gagatggacg atccggcgct gcacattgcc gatgcgcgtt accagtgcat
    41701 tgctgccatc tgtgatgtgg taagcaacac cctgacggca gaacccagcc gtttcaccac
    41761 tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc tctttgtgat
    41821 gtacctgatg ttcctgctgg ctatcaacat cggcggggcg ttacagccgc tgtttgacgt
    41881 cggctccgtg gcgctatttg tgcatggtat tcaatggatt ggctacacgc tccacttccc
    41941 ggactggctg actatcttcc tcgcccaggg cctgggtggc ggcattaaca ccgtgctgcc
    42001 actggtgccg cagattggca tgatgtacct gttcctctcc ttccttgagg actccgggta
    42061 tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag gcgctgggct tgccggggaa
    42121 atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta ccgtcggtaa tgggtgcacg
    42181 tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg atggcaccgt ttatgtcctg
    42241 cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc ttcttcgggc agaacggtgc
    42301 gctggcggtc ttctcgctgt atatgctggg tattgtgatg gcggtgctga ctggcctgat
    42361 gctcaagtac accatcatgc gcggtgaagc gacgccgttt gtcatggagt tgccggtcta
    42421 tcatgtacca cacgttaaaa gcctgattat ccagacctgg cagcgtctga aaggcttcgt
    42481 tctgcgtgct ggtaaagtga tcatcatcgt cagcattttc ctgagcgctt tcaacagctt
    42541 ctcgctgagc gggaaaatcg tcgataacat caacgactcg gcgctggcgt ccgtcagccg
    42601 ggtgatcacc ccggtcttca agccaattgg cgtgcatgaa gataactggc aggcaacggt
    42661 tggcctgttt acaggtgcca tggcgaaaga agtggtagtg ggtacgctca acaccctcta
    42721 caccgcagaa aatattcagg acgaagagtt caatccggca gagtttaacc tcggtgaaga
    42781 gctgttcagt gcgatagatg aaacctggca gagcctgaaa gacaccttca gccttagcgt
    42841 actgatgaac cccattgaag ccagcaaagg cgacggcgaa atgggtaccg gggcgatggg
    42901 cgtgatggat cagaaattcg gtagcgcagc agcagcttac agctacctga ttttcgtcct
    42961 gctgtatgta ccatgtatct cggtgatggg agccatcgcc cgtgaatcaa gccgtggctg
    43021 gatgggcttc tccatcctgt gggggctgaa tatcgcttac tcgctggcaa cattgttcta
    43081 tcaggtcgcc agctacagtc agcacccaac ttacagcctg gtgtgcattc tggcggttat
    43141 cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg cgtagccggg tggatatcga
    43201 actgctggca acccgcaagt cggtaagtag ttgctgcgca gccagcacca ccggtgattg
    43261 ccattaatgg cttcacttat tcaggtgcgt gatttgctgg cgttacgggg ccgtatggaa
    43321 gcggcccaga taagccagac attgcacact ccgcagccaa tgattaacgc catgctgcaa
    43381 caactggaaa gtatgggcaa agccgtgcgg attcaggaag aacctgacgg ctgcctctcc
    43441 ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc gcgagtggtg ggcgctgcgt
    43501 taaccatact ccatcgcctg ttttgaaaag cccggtatgc gtctgcatcc gggctttttt
    43561 gcgtgcggct ttccataaaa atgcaattct tgcagcgcga cgtaagttcc tctgaaagcg
    43621 tctcgcaggg atgaaaactg gctaatgcac aggtgtggag tggcgcgtag agtcgcggca
    43681 ttcaaacaac aggtgaagga acgccatgag caaaaagcag agttccaccc cacacgatgc
    43741 gctgttcaaa ctctttttac gccaaccgga gacggcacgc gattttcttg cgtttcattt
    43801 accggcaccc attcacgcgc tctgtgatat gaaaaccctc aagctggagt cgagcagctt
    43861 tattgatgac gatctgcgtg aaagctattc cgatgtgctg tggtcggtga aaacagaaca
    43921 aggaccagga tacatctatt gtctgattga acatcaaagc acctcaaaca aactgatcgc
    43981 atttcgcatg atgcgttacg ctattgccgc aatgcaaaat caccttgatg ccggatacaa
    44041 aacgttgccg atggtggtgc cattgttgtt ttaccacggt attgaaagcc cctatcccta
    44101 ttcgctgtgt tggctggatt gtttcgccga tcccaaactg gcaaggcagc tttatgcctc
    44161 cgcatttccg ctgattgatg tcaccgtcat gcctgatgat gaaatcatgc agcaccgacg
    44221 tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa cgcgatctga tggggctggt
    44281 agaacaaatg gcctgcttat taagtagtgg atacgctaat gacagacaaa tcaaagggct
    44341 gtttaattac atcttgcaaa ccggcgatgc ggtacgtttt aacgatttta tcgacggcgt
    44401 tgccgaacgt tcaccgaaac acaaggagag tttaatgact attgcggaaa gattgcggca
    44461 ggagggggaa caatccaaag ccttgcatat agccaaaata atgcttgaat ccggagtccc
    44521 tcttgcagac atcatgcgct ttaccgggct gtcagaagaa gagttggctg cggcgagtca
    44581 gtaaagttct gtctcgccat tcaaaagtgc ctacaccctc tgcttcaacg ccaccagcac
    44641 gtgacgaaac tcgaccggat gcgaaataaa cggcgcatga gccgctttgg cgaagatgta
    44701 tgactcgctg tgtggccaga gtttatccag catcggcacc acttttcgcg gcaccagacc
    44761 atcgagatag ccatacaatc gcaaaaacgg catggacacg ttttgcagcg gctggcggag
    44821 atcgaccgtt ttcaggattt ccagcccgcc attaagcacg tcaacctccg gcatcggtaa
    44881 cgccagaacg gttttcttca acgcccgcgc atcctgacgc gccgtttcag tacccatggt
    44941 ttgtaacgca aggaaccgtt ccaccgtacg ctgaaaatca tcactgagtt gctgctgaaa
    45001 tcccgccagc acgtccggtt ttatccccgg ccactcgtca cgagcactaa aacaaggtga
    45061 cgacgccacg gtgaccagcg cctgaacacg ctcgggatgg gttaacgcaa tctggcttgc
    45121 caccagcccg cccagactcc agcctaacca aatggcttta tcaggtgcct gtcgcagcac
    45181 ggcttcggcc atatcagcaa gtgacatcgc accaaatccc cggctacgcc cgaagccggg
    45241 caggtcaaca aggtgcagcg taaaatgcga gctcagttcc tcgtcaatgc aacgccacac
    45301 ttcggcattc agtccccatc cgtgcagcag cacaagatga acattcccct gacctttggt
    45361 ctgccaccag atgttattca tccgctattg ttctcttttg acttacaagg atgaacatat
    45421 gctaacagta ccaggattat gctggctatg ccgaatgccg ctggcgttag gtcattgggg
    45481 gatttgttcg gtctgctcac gcgccgcccg tacagataaa acgttatgcc cacaatgtgg
    45541 attacccgcc acacactcac atcttccctg cggtcgctgc ctgcaaaaac caccgccctg
    45601 gcaaagactg gtcacggttg ccgactatgc gccgccgtta agcccgctaa tccaccagct
    45661 taagttttcc cggcgcagcg aaatcgccag cgccctgtca cgtctgttac tattggaagt
    45721 cttacacgct cgtcgcacca ccgggttgca attgccggat cgtatcatca gcgttccgtt
    45781 atggcagcgg cgtcactggc gtcggggatt taatcagagc gatttgctgt gtcagccgtt
    45841 atcacgctgg ttgcactgcc aatgggatag cgaagccatc acacgaacac gggccactgc
    45901 gacccagcat tttctcagtg cccggctacg caagcgcaac ctgaaaaatg cctttcgtct
    45961 tgaattgccc gtgcaaggtc gccatatggt gattgtggat gatgtcgtta ccaccggaag
    46021 taccgtcgca gagattgcgc agttgctttt acgcaatggt gcggcgactg tccaggtctg
    46081 gtgcctttgt cgaaccttgt agagcctcga tgatgggcgt attataacca actaaaatag
    46141 tcaactatta ggccattact atgatccgta tttccgatgc tgcacaagcg cactttgcca
    46201 aactgctggc aaatcaggaa gaagggacac aaatccgcgt atttgtgatt aaccctggca
    46261 cgcctaacgc tgaatgtggt gtttcttatt gtccgccgga tgccgtggaa gccaccgaca
    46321 ccgccctgaa atttgacctg ctgaccgcgt atgttgatga gttaagcgca ccatacctgg
    46381 aagatgcaga gatcgatttt gttaccgacc agttgggttc ccagttaacg ctgaaagccc
    46441 cgaacgccaa aatgcgtaaa gtggcagacg atgcaccgct gatggagcgc gtggagtata
    46501 tgctacagtc gcagatcaac ccgcaacttg ctggtcacgg tggtcgcgtt tcgctgatgg
    46561 aaatcaccga agacggttac gccattctgc aatttggcgg cggctgtaac ggttgttcca
    46621 tggtggatgt gacgctgaaa gaagggatcg agaagcagct gctaaacgaa ttcccggagc
    46681 tgaaaggtgt gcgcgatctc accgaacacc agcgcggcga acactcctac tactaagtta
    46741 tcccctcttt tgtagattgc ccgatgcgac gctaaagcgt catttcgggc ctacattgaa
    46801 catgtgccag acgttggcgc agcattgctt tggcaaatcc ctcccttcgt ttccccgcga
    46861 taatatgacc aacctctcat aatttaaatt taccccgctc tggtgattct caaacgccag
    46921 atgttacccg tatcattcac atgggtacca aacatactcc tgacatctga ctacaataat
    46981 tagttttagt gggtatcagt cgtggtgccg caatatctct gttcccgatt gggataatta
    47041 gagtttgtcg tcagaaaatt gacgttaccc ataacaaatg aaaggccagg taaatcatgc
    47101 cattagtcat tgttgctatc ggtgtaatct tgttgttgct cctgatgatc cgcttcaaaa
    47161 tgaacggctt catcgctctc gtcctcgtgg cgcttgctgt tggattaatg caaggaatgc
    47221 cgctggataa agttattggc tccatcaaag ccggtgtcgg cgggacgctc ggtagccttg
    47281 ccctgatcat gggttttggc gcaatgctgg gcaaaatgct ggcagactgc ggtggcgcac
    47341 aacgtatcgc caccacgctg attgccaaat ttggtaaaaa acacatccag tgggcggtgg
    47401 tactgaccgg ttttaccgtt ggttttgccc tgttctatga agtgggcttt gtgctgatgc
    47461 tgccgctggt gtttaccatc gcggcttctg cgaatattcc actgctgtat gttggtgtac
    47521 caatggcggc tgcactgtct gtgacccacg gcttcctgcc accgcatccg ggcccgactg
    47581 cgattgccac cattttcaat gccgacatgg gtaaaaccct gctgtacggt actattctgg
    47641 caatcccgac cgtgattctc gccggtccgg tttacgctcg cgtgttgaaa ggtatcgata
    47701 agccaatccc ggaaggtctc tacagcgcga aaaccttcag cgaagaagag atgccgagct
    47761 ttggcgtcag cgtctggacc tctctggtgc cggtagtact gatggcgatg cgtgcgattg
    47821 ccgaaatgat cctgccgaaa ggtcacgctt tcctgccggt agcggagttc ctcggtgacc
    47881 cggtaatggc tacgctgatt gccgtgctga ttgcgatgtt cacctttggt ctaaaccgtg
    47941 gtcgttcaat ggatcagatt aacgacacgc tggtttcttc catcaaaatc attgcgatga
    48001 tgctgttgat catcggtggt ggcggtgcgt tcaagcaggt gctggtagac agcggcgtgg
    48061 acaaatacat tgcttccatg atgcacgaaa ccaacatttc tccgctgctg atggcctggt
    48121 cgattgctgc cgtactgcgt atcgcgctgg gttccgcaac cgttgcggca atcactgcgg
    48181 gtggtatcgc ggcaccgctg attgcaacga cgggtgttag cccggagctg atggttattg
    48241 cggttggttc cggtagtgtg attttctctc atgtgaacga tccgggcttc tggctgttca
    48301 aagagtactt taacctgact atcggcgaga ccatcaaatc ctggtcgatg ctggaaacca
    48361 ttatctcggt gtgcggtctg gtaggctgtc tgctgctgaa tatggtgatt tgataacact
    48421 gactgccgga tgcatcgcat ccggcatcaa ccgcactcta cttcttcttc gctgcagctc
    48481 tgcgccgtct gtccaaatcc ttcagcaact tgttcacgcc atcatcggca aacatcgtct
    48541 caagcgttgc ggaaagcttg cgtcgccagt ttttatactg gtaactggtg ccaggaatat
    48601 tcaccggttc ggccatatcc agccagtctt ccggctgtag tcctaacaga gcactgttac
    48661 tgtcggcaat gtagcgctgc aaaccacggt tcagcgtcgg cgtcatcgac atcaacgatg
    48721 ccttatgccc ggcacgtttt ggcagacaac catatttatg cagtgcatcc agcagccctt
    48781 gcttcgccag ttcgcgatcc tgatacagac cgcgcagtac cacttcatcc ggatacagcc
    48841 ccagggtttt gcccagcgtt agatccccgc tctcccagta accgcgtagc gtaggcaggt
    48901 catgtgtcgc cgcaaccgcc atcgactgct ccggatacgc tttcggtgca cggaacgtct
    48961 tctcgtggtc gttttcgaaa tagagcactt tgtaggagta cacaccgctg ctgcgcagct
    49021 taccgacaat ctctaccggt acggtaccga gatcttcacc aatcaccata cagcgatgac
    49081 gtttactttc gagtgccaga atcgagagca gatcatccac cggatagtga acatacgcgc
    49141 cctgatctgc cgtctcgcca tacggtatcc accacaaacg cagcatcgac atcacatggt
    49201 caattcgtaa tgcgccgcag ttttgcatat tggcacgcaa cagctcgata aacggttcat
    49261 aggcacgcgc ggtgatgata tgcgggtcca ttggcggtaa tccccagttc tgccccaacg
    49321 ggccgaggat atccggcggc gcgccaaccg acgctttcag gcaatacagt tcacggtcgc
    49381 accaggtttc cgccccacct tccgctacgc caaccgccag atcacgatac aagccaatcg
    49441 gcatttcata gccctggctt atctcccagc aggcggcaaa ctggctgtaa gccagccact
    49501 gcaaccagag ataaaaatcg acgtcatcac gatgttcttc gcagaactga cgcacttctg
    49561 gtgaatccac gttctgatac atctctggcc atgcaggcca gccccagcgc atttcgtcct
    49621 ctttcacttg ctgggcatgt agcgcatcaa aggctgcctg ccagaacagg ctgtcgcctt
    49681 gatctgcaac aaactggcga aacgcggtca tctgctcatc atcacgttgc gcgaaacctt
    49741 tccacgccat tcgtaatgct gtcattttta gggcggtaac cgtggagtaa tcgacccagt
    49801 cggcatcccg cgcctgttgc agcgtctgtt gcgtggtcgg caactgccac caggcctgag
    49861 cctcttcgct aagatggaaa tcttcgacgg cgttaacgtc gatataaatc acattcagcc
    49921 aacggcgaga agacgggctg tatgggctgg cactctccgg atttgccgga tagagcgcat
    49981 gaatcgggtt caggccgatg aacgacccgc cacgttttgc cacatccacc agcatcgctt
    50041 tgagatcgcc aaaatcccca ataccccagt ttttttccga tcgcagcgta taaagctgaa
    50101 cgcaggcacc ccacagcttt tgtttattca gcaacgcctg cggttcgtaa cagcgtttcg
    50161 gggcgacaat cacccggcaa tgcgcgcgct ggtcgtcctg ggtgagtgtc agcgtgtgat
    50221 aaccttccgg cagcttcgtc ggtagattga acgctttgcc cccagttaca tggcctttgt
    50281 actgcgttcc ttcttcggtg gtcagcagcc agctatattc gccgctgccc tccaccacca
    50341 tcggcatttt tttgccgctg gtataaacca tgacattcgg gactggcgtt accgccactt
    50401 tcgtggcggt acgttgatgc atcgcgtcaa gcaaacgccg tttggtttcg gcgctaatcg
    50461 actgcggttt accgtgggca ttgatgtaat tggggctaat ccccgccgcc agcgcggcat
    50521 tatccagacg tttgctttcc atcgagcttc cttagcgttt tgcctgccag atacgagcct
    50581 gataatcgcg aatagagcga tccgagctaa acataccgca gcgggcggta ttgaggatcg
    50641 ccgcgcgagt ccaggcctcc tggtcgcggt acagcacatc cacctgcttt tgtgcctcta
    50701 cataagctgc gaaatccgcc atcaccagat acggatcgcc gccctgtttg ccgatactgt
    50761 gcagcatctg gtcgaaggca tgcttatcgc cgtcgctgta tttgccgctt tccagctctt
    50821 tcaatactgc gtccagcacc ttatctttct tccgccattt caccgggtcg tagcctttgg
    50881 ccagaattgc cttcacttgt tccacggtat gaccaaaaat aaagatattt tcttcaccga
    50941 ctttctcggc gatttcaacg ttcgccccat ccagcgtacc gacagtaagc gcaccattga
    51001 gcgccagttt catattgccg gtaccggaag cttctttacc tgcagtcgaa atttgttcgg
    51061 agatatccgc cgccgggatc agtttttccg ccgccgaaac gcaataatcc ggcaggaaca
    51121 ccaccttcaa cttatcgcca accagcggat cgttgttgat cacgtcagcc actttgttga
    51181 tcgcaaagat aatattcttc gccaggtagt agcccggtgc cgctttcgcg ccgaagagga
    51241 agacgcgcgg tacgcgatca gcctgcgggt tttcacgaat ttctttgtac aacgccagaa
    51301 tatgcagcag attcaggtgc tggcgtttgt actcatgcag acgtttgatc tgaatatcga
    51361 aaatcgcctg tggattgatc tcaataccgg tacgaacttt cacaaactcc gccagacgga
    51421 ctttattcgc ctgcttgatc tcgcgatatt gctgacggaa tttcgcatca tcagcgaatt
    51481 tttccagatt gatcagctga tcgagatcgt tagcccactc ttttttcagt gatttatcca
    51541 acagagccgc cagtgccggg ttgcactgtt tgatccagcg acgtggggta ataccgttgg
    51601 tgacgttatg gaatttgttc ggccatagct ggtgatattc cgggaacaga tctttcacca
    51661 ccagatccga gtgcagcgcc gcaacaccgt tcaccgcgaa accgccaacc acacacaggt
    51721 tcgccatatg cacttgttta tcgtgcacca ccgccagttt ggcccacact ttttcatcgc
    51781 ccggccaggt tttctccacc agcgttttaa agcgagtatt aatttcgtta ataatctgca
    51841 tatggcgcgg cagtaagcct ttcaccagtt tcacctccca gcgttccagc gcttctggca
    51901 tcagggtatg gttggtgtaa gcgaaagttt tgctggtaat ggcccaggcg tcatcccagc
    51961 tcatctggtg ctcatcgatc agcacgcgca gcagttctgg aatcgcgata gttgggtggg
    52021 tatcgttcag ctgaataact tcgtaatccg ccagttcgtg cagtttacgc cccgccagat
    52081 gatgacgacg caaaatatcc gctaccgaac aggcacactg gaagtactgc tgcatcaagc
    52141 gcagcttttt accggcagta tggttgtcgt ttggatagag aactttggtc agtttttccg
    52201 cattgatgcc ctgctgttcg gcacgcagga aatcaccgtc gttaaattta gtcagatcaa
    52261 acggatgcgc gtgcgtcgcc tgccacagac gcagcggctg cgccacgcca ttacgatagc
    52321 cgacaacggg gagatcccac gcttgaccgg taatggtaaa ctccggctcc cagcgtccgt
    52381 ctttcgtcac tttaccgcca atccctacct gcacatccag tgcttcgttg tggcggaacc
    52441 acgggtagtt actgcgatgc cagtcatccg gcgcttcaac ctgtttgcca tcgacaaaag
    52501 actggcggaa caaaccatat tgatagttca gaccgtaacc cgtcgcagac tgaccgacag
    52561 ttgccattga gtcgaggaag cacgccgcca gacgtcccag accaccgtta cccagcgccg
    52621 ggtcgatctc ttcttccagc aggtccgtca gattgatgtc ataagccttc aacgaatcct
    52681 gtacatcctg ataccagccg agattcaaca ggttgttgcc cgtcaggcga ccaatcaaaa
    52741 actccattga gatgtagtta acatgtcgct gattcgccac cggcttggcg aatggctgag
    52801 cacgcagcat ttcggccagt gcttcactca ctgctagcca ccactggcga ggagtcattt
    52861 cagccgcaga atttaagcca taacgctgcc actgacgtga aagcgcttcc tgaaattgct
    52921 tatcgttaaa aataggttgt gacataggag ttccactttt cttagatttt caacacaacg
    52981 ttatcgctag tttgccaggc tcgatgttga ccttcctcat cctgcggggg attaggcagg
    53041 gaggagttgc ggggatgagc aaggaaatgt gatctcaacc acttaaagct agtgcaaacc
    53101 acaggattag cagcaaatca atgcaataca gcgcagaaaa tctgtatcta agtgcaaaaa
    53161 atggccgttg cgtattttca aaaagcggaa ggtaactcta taaattaagt aaaggagtga
    53221 aacagtttca caagtaaaat atccagtgtg ctccatctca ttcttaatag atttattaag
    53281 atcatctttt tagatggcac tttcatcagg aatgaagaag aaacccttgc ttaaatgaat
    53341 ctgatgaaca taagggaaac cagtattcac gctggatcag cgtcgtttta ggtgagttgt
    53401 taataaagat ttggaattgt gacacagtgc aaattcagac acataaaaaa acgtcatcgc
    53461 ttgcattaga aaggtttctg gccgacctta taaccattaa ttacgaagcg caaaaaaaat
    53521 aatatttcct cattttccac agtgaagtga ttaactatgc tgattccgtc aaaactaagt
    53581 cgtccggttc gactcgacca taccgtggtt cgtgagcgcc tgctggctaa actttccggc
    53641 gcgaacaact tccggctggc gctgatcacg agtcctgcgg gctacggaaa gaccaccctc
    53701 atttcccagt gggcggcagg caaaaacgat atcggctggt actcgctgga tgaaggtgat
    53761 aaccagcaag agcgtttcgc cagctatctc attgccgccg tgcagcaggc aaccaacggt
    53821 cactgtgcga tatgtgagac gatggcgcaa aaacggcaat atgccagcct gacgtcactc
    53881 ttcgcccagc ttttcattga gctggcggaa tggcatagcc cactttatct ggtcatcgat
    53941 gactatcatc tgatcactaa tccagtgatc cacgagtcaa tgcgcttctt tattcgccat
    54001 caaccagaaa atctcaccct ggtggtgttg tcacgcaacc ttccgcaact gggcattgcc
    54061 aatctgcgtg ttcgtgatca actgctggaa attggcagtc agcaactggc atttacccat
    54121 caggaagcga agcagttttt tgattgccgt ctgtcatcgc cgattgaagc cgcagaaagc
    54181 agtcggattt gcgatgacgt ttccggttgg gcgacggcac tacagctaat cgccctctcc
    54241 gcccggcaga atacccactc agcccataag tcggcacgcc gcctggcggg aatcaatgcc
    54301 agccatcttt cggattatct ggtcgatgag gttttggata acgtcgatct cgcaacgcgc
    54361 cattttctgt tgaaaagcgc cattttgcgc tcaatgaacg atgcactcat cacccgtgtg
    54421 accggcgaag aaaacgggca aatgcgcctc gaagagattg agcgtcaggg gctgttttta
    54481 cagcggatgg atgataccgg cgagtggttc tgctatcacc cgctgtttgg taacttcctg
    54541 cgccagcgct gccagtggga actggcggcg gagctgccgg aaatccaccg tgccgccgca
    54601 gaaagctgga tggcccaggg atttcccagc gaagcgattc atcatgcgct ggcggcaggc
    54661 gatgcgctga tgctgcgcga tattctgctt aatcacgcct ggagtctgtt caaccatagc
    54721 gaactgtcgc tgctggaaga gtcgcttaag gccctgccgt gggacagctt gctggaaaat
    54781 ccgcagttgg tgttattgca ggcgtggctg atgcaaagcc aacatcgcta cggcgaagtt
    54841 aacaccctgc tagcccgtgc tgaacatgaa atcaaggaca tcagagaaga caccatgcac
    54901 gcagaattta acgctctgcg cgcccaggtg gcgattaacg atggtaatcc ggatgaagcg
    54961 gaacggctgg caaaactggc actggaagag ctgccgccgg gctggttcta tagccgcatt
    55021 gtggcaacct cggtgctggg tgaagtgctg cactgcaaag gcgaattgac ccgctcactg
    55081 gcgctaatgc agcaaaccga acagatggca cgccagcacg atgtctggca ctacgctttg
    55141 tggagtttaa tccagcaaag tgaaattctg tttgcccaag ggttcctgca aaccgcgtgg
    55201 gaaacgcagg aaaaagcatt ccagctgatc aacgagcagc atctggaaca gctgccaatg
    55261 catgagtttc tggtgcgcat tcgtgcgcag ctgttatggg cctgggcgcg gctggatgaa
    55321 gccgaagcgt cggcgcgtag cgggattgaa gtcttgtcgt cttatcagcc acagcaacag
    55381 cttcagtgcc tggcaatgtt gattcaatgc tcgctggccc gtggtgattt agataacgcc
    55441 cgtagccagc tgaaccgtct ggaaaacctg ctggggaatg gcaaatatca cagcgactgg
    55501 atctctaacg ccaacaaagt ccgggtgatt tactggcaaa tgaccggcga taaagccgcc
    55561 gctgccaact ggttgcgtca tacggctaaa ccagagtttg cgaacaacca cttcctgcaa
    55621 ggtcaatggc gcaacattgc ccgtgcacaa atcttgctgg gcgagtttga accggcagaa
    55681 attgttctcg aagaactcaa tgaaaatgcc cggagtctgc ggttgatgag cgatctcaac
    55741 cgtaacctgt tgctgcttaa tcaactgtac tggcaggccg gacgtaaaag tgacgcccag
    55801 cgcgtgttgc tggacgcatt aaaactggcg aatcgcaccg gatttatcag ccattttgtc
    55861 atcgaaggcg aagcgatggc gcaacaactg cgtcagctga ttcagcttaa tacgctgccg
    55921 gaactggaac agcatcgcgc gcagcgtatt ctgcgagaaa tcaatcaaca tcatcggcat
    55981 aaattcgccc atttcgatga gaatttcgtt gaacgtctgc taaatcatcc tgaagtacct
    56041 gaactgatcc gcaccagccc gctgacgcaa cgtgaatggc aggtactggg gctgatctac
    56101 tctggttaca gcaatgagca aattgccgga gaactggaag tcgcggcaac caccatcaaa
    56161 acgcatatcc gcaatctgta tcagaaactc ggcgtggccc atcgccagga tgcggtacaa
    56221 cacgcccagc aattgctgaa gatgatgggg tacggcgtgt aagtttagcc ggataacgcg
    56281 ccagatccgg cttacatctc tgcatcattc aatgctcacc cgcgttacgc catctgtttc
    56341 tatcaaacta aaccgcaccg gcaagaaacg ctccaccacc gcgatattgg tcagcagatg
    56401 gcatgaggga tgggcgaccg taaattcccc cgcgcccgcc agcgccatcg gtagcaccag
    56461 ttggtcagca agatattccc ccaccgccgc cgtgcttgcc aggtagcgtt tcacctcttt
    56521 caccaactgt gccgcaacca cctcggcact gacgcgcttt tcaccgacga caaaaagcgt
    56581 tcggtgatat tttcactttc gacttcaagc gagacggtat tacccggccc ctggtcgcgc
    56641 ggcaggttat gaatattctg ttcatgcagg gaaaaactcc ccgccagtgt agcgatttca
    56701 cgctcagcaa catggcgcgg cacaccagct aatagaactt ctccacgcat ctgcacaatg
    56761 ttcccgcgct cgccaagttg caaggtgtta aacgatgcaa cgggcgagac ttccgttgcc
    56821 accacaccgc ctccggcagg gtaaaaaccg tggcgtaaca gcgtggtttg ctgatgaatt
    56881 cccattttcg ccagcagcgg ctccagcacc cggcggataa aatccgcagg tggcgccgac
    56941 gggttatcgg taccgccgct cacttcaaca cgcgaaggtc catcggcaaa ccacagcgcg
    57001 ggcagcacag tttgcagcac cagcgtacaa cttccggcgc taccgatagc aaagcggtaa
    57061 tcgccgccgc gcacggtgcc gggccggaag agcagacgct gcgatcccag ctccgcacct
    57121 tccaccattg ccctacaaat ttccgcagcc gcttttaccg cggtcagatg ctggcgcagc
    57181 agccccggtt ttgcccgccc ggcacgaatg ccagtgatgg taaatggctg gccggttatc
    57241 atcgacaggc tcagcgccga gcgcaggatc tgtccgccgc cttcgccctg tgcgccatcc
    57301 agcgcaatca tccttttcat catttatcct tttacgcaca ccacctgacg cagggtgtag
    57361 ataacttcca ccagatcgct ttgtgccgcc atcaccgcat caatatcttt atacgccatc
    57421 gggatttcgt cgatcacttc ggcatcttta cggcattcca catgcgcggt ggcacgaatt
    57481 tgatcttcca cgctgaacag ttttttcgct ttagtacggc tcattacccg cctggcaccg
    57541 tggctgcacg aacagaacga ctcttcattt cccagcccac ggacgataaa gcttttcgct
    57601 cccatcgaac cgggaataat tccatattga ccagcacgcg cagacaccgc gcctttacgc
    57661 gtcacgtaga tctcttcgcc aaagtgctgt tctttttgca catagttgtg gtgacagttg
    57721 atctcttcca tcgccagcgt ttgctgttgt cttaccgttt tccgcgtaac gctctgcaac
    57781 gctgttacta cgttttccat cattgcatcg cggttaaggc tggcaaaaag ctgcgcccag
    57841 gccaccgctt tcaggtaatc atcaaagtat tccgtacctt ccataaagta cgccagatca
    57901 cgcgacggca acgtttcaag ctggtcctgc atctcttttt gcgccagatc gataaagtaa
    57961 gtcccgatgg cgttaccaat tccgcgtgaa ccggagtgca gcataatcca cacctggtcc
    58021 gactcatcaa ggcagatttc aataaagtgg ttacccgttc ccagcgttcc caggtgttta
    58081 tagttgttag tgttaaggaa acgcggatat ttttgcgtta accactgata accggcctcc
    58141 agctcagccc atttcgcatc gacgttaaca ggcgggtttt cccatgcgcc tttatcacgt
    58201 ttacaacggc cagtggtacg cccgtgcggc acggccgttt caatcgcctg acgcagctct
    58261 gccaggtttt caggcaggtc tgccgccgtt aacgcggtac gcagcgcgtt cattccacag
    58321 ccaatatcca cgcccaccgc cgccggaata atcgcccctt tggtcgggat cacgctacca
    58381 atggtggaac ctttacccag gtgtacatca ggcattaccg caatatgttt gaaaataaac
    58441 ggcatcttcg ccgtattaat aagttgctga cgcgcatcgg cttctaccgg cacgcctttg
    58501 gtccacattt ttaccggggc attttcagtg gtcagtaatt cgtaattcat tttgtttttc
    58561 tcttttcgtt gtttgctgtc gtgataattg caaccgtcgt gccagaaaat taaataagca
    58621 gattaatttt ttaattcatt gtttttaaat aggttaaatc tttacgtccg taaacggaga
    58681 ttttccacaa aaccaattca ccgataatga aatatcgtct tttataagga tatctaagat
    58741 gcgtaaaaca gtggcttttg gctttgtcgg aacagtgctg gattatgccg ggcgcggcag
    58801 tcagcgctgg tcgaaatggc gtccgacact ctgtttatgc cagcaagaat cgttggtcat
    58861 cgatcgactg gaattattgc atgacgcccg ctcgcgctcg ctatttgaaa cgcttaaacg
    58921 cgatatcgcc agcgtttcgc cagaaacaga agtggtgagc gttgagattg aactgcataa
    58981 cccgtgggat ttcgaagagg tttacgcttg cctgcatgat ttcgcccgtg gttacgagtt
    59041 tcagccagaa aaagaagact atttaattca cattaccacc ggcacccacg tcgcgcagat
    59101 ttgctggttt ctgctggcgg aagcgcgtta cctgcccgcc cggctgatac aatcttcacc
    59161 tccgcgcaaa aaagaacagc cgcgcggcgc aggtgaagtg acgattatcg atctcgattt
    59221 aagccgttat aacgccatcg ccagccgctt tgccgaggaa cgccagcaaa cgcttgattt
    59281 tcttaagtcc ggcattgcca cgcgtaaccc ccacttcaac cgcatgattg agcagatcga
    59341 aaaagtggcg atcaaatccc gcgcgccgat tctgcttaac ggtccaaccg gcgcgggcaa
    59401 gtcatttctg gcgcgacgca tcttcgagtt aaaacaggcg cggcatcagt ttagcggcgc
    59461 gtttgtggaa gtgaactgcg ccaccctgcg cggcgatacc gccatgtcga cgctgtttgg
    59521 tcatgtaaaa ggcgcgttta ccggggcgcg ggaatctcgt gaaggtttat tacgcagcgc
    59581 caacggcgga atgttgtttc ttgatgagat tggcgaactg ggcgcagacg aacaagcaat
    59641 gctgctaaaa gccattgaag agaaaacctt ttacccgttt ggcagcgatc gccaggtgag
    59701 cagtgatttt cagcttatcg ccggaacggt gcgcgatttg cgccagctgg ttgccgaagg
    59761 caaattccgc gaagatctgt acgcgcggat caatctctgg accttcaccc tgccggggct
    59821 acgccagcgc caggaagata ttgaacctaa cctggattat gaagtggagc gccacgccac
    59881 actcactggt gacagcgtgc gttttaacac cgaagcgcgg cgtgcctggc tggcctttgc
    59941 aacatctccc caggcgacat ggcgcggtaa ctttcgcgaa ctttctgcca gcgtcacgcg
    60001 gatggcaacc tttgccacta gcggacgcat cactctggac gtggtggaag atgagataaa
    60061 ccgtctgcgc tataactggc aggaaagtcg cccttccgtg cttacgcagc tgctgggcgc
    60121 tgaagcggaa aatatcgacc tctttgatcg gctgcaactg gaacacgtta tcgctatctg
    60181 ccgccaggca aagtcgcttt ctgctgccgg acgacagctt tttgatgttt cgcgccaggg
    60241 caaagccagc gtcaatgacg cggatcggct acgcaaatac ctggcgcgtt ttggtctgac
    60301 gtgggaagcc gtgcaggatc agcacagctc cagttgaata tggtggtccg tcagcacctg
    60361 catcacgctt actggcggcg gggcgtcggt gtagacggca tctaccatgc tgatgctgcc
    60421 catattgacc atcgcgttac ggccaaattt cgagtgatcg acaaccagca taacgtggcg
    60481 cgagttctca ataatggcgc gtttggtgcg aacttcgtgg taatcgaact ccagcagcga
    60541 gccgtcgcta tcgatgccgc ttatccccag aatgccgaaa tcaaggcgga actgggagat
    60601 aaaatcgagc gtcgcttcgc caatgatccc gccatcgcgg ctgcgtaatt cgccaccggc
    60661 gagaataatg cggaaatctt cttttaccat caacgtgtta gcaacattga gattgttggt
    60721 gacaatacgc aaattgctgt gattaagcag cgcgtgcgcc accgcttccg gcgtggtgcc
    60781 gatatcgata aacagtgtcg agccattggg gatttgctcc gccactttgc gggcaatcct
    60841 ctctttttct tcggtctggg tggctttacg atcgtgccac ggcgtgttaa ccgaactgga
    60901 aggcagcgcc gcaccgccat ggtgacgcag gatcaggttt tgctccgcca gctcattgag
    60961 gtcgcggcga atagtctgcg ggctgacgga gaagtgctcc accagctcct cggtactgac
    61021 ataaccctgc tgtttaacca gttcgataat accgttgtga cgttgtgttt gtttcatttc
    61081 taaatccctg gaattatttt cgttttcgcg cattgagcga atcaacaaaa gccatcgcta
    61141 aacccacggc taacccggcg atgtgtgctc cgttcgccat cgacatccca aacaaatcaa
    61201 accatccggc gacaatccag atcagcgcaa agataattaa cccacgttgc aggtaaatgc
    61261 cactttgcgg atcgcgttcg ccacgtagcc agacgtagcc catcagcgca tacaccacgc
    61321 cagaaagccc gccaaaccac ggcccgctga atttttgctg cacatagccg cttaacaggg
    61381 cgctaatgag agtaataaca attagcttac cgctaccgag gcgtttttcc accgcgccgc
    61441 cgagatacca ccaccagagc aggttaaaga ggatatgcat cagcgagaag tgcattaacg
    61501 cgtgggtgaa gtaacgccag aactcaaatt tcagtgttgg atcgaatggc caggccagcc
    61561 ataacatcac ttcctgatcg ccgagaattt gcatggcgat aaacaccacc acgcaggcga
    61621 tcatcatcac ccaggttacc ggacctgcgc gttcacgcaa ggcagcaaag aaaggataac
    61681 ggcgataatg caggccactg ccggtatgac ccgcctgcca gctggccgcc agataacgcg
    61741 gatctgccgg gttttcgaga aaacgcgcca gctccgcccg cacgcgctcg gcctgggact
    61801 catccgccag ccagacgtcg ctttggttat gttgttgaat cgtgaggata acaccctgcg
    61861 tcgccatgta atcaacaaac gcctgcgcca cgcgggggtt agcaaaagag gtaatcatca
    61921 acatcgttgc tgtcgcttat tccacacaaa aggggacagt ataaagcgtt acgcgccgta
    61981 cgccacctct gcgggaaact gtcgctgcca ggcttcaaag ccgccgtcaa tgctatagac
    62041 cacatcgtag ccctgttgca gcagatactg cgccgcccct ttgctgctat tgccgtgata
    62101 acacatcacc atcaccggag tgtcaaagtc gttatcacgc ataaacgcgc ccagcgtgtc
    62161 gttggttaaa tggaaagcct gcaccgcatg ccccatagcg aaactctgtg gatcgcgaat
    62221 atcgaccagc accgcctctt tttcctgcaa cttctggtgc gcgtcggcaa cgttaataca
    62281 ttcgaactga tccatgcgtc tctctttctt tacaaacaag tgggcaaatt taccgcacag
    62341 tttacgtcga agcggcagat aaacgccata atgttataca tatcactcta aaatgttttt
    62401 tcaatgttac ctaaagcgcg attctttgct aatatgttcg ataacgaaca tttatgagct
    62461 ttaacgaaag tgaatgaggg cagcatggaa accaaagatc tgattgtgat aggtggcggc
    62521 atcaatggtg ctggtatcgc ggcagacgcc gctggacgcg gtttatccgt gctgatgctg
    62581 gaggcgcagg atctcgcttg tgcgacctct tccgccagtt caaaactcat tcacggtggc
    62641 ctgcgctacc ttgagcacta tgaattccgc ctggtcagcg aggcgctggc tgaacgtgaa
    62701 gtgctactga aaatggcccc gcatatcgcc ttcccgatgc gttttcgcct gccacatcgt
    62761 ccgcatctgc gcccggcgtg gatgattcgc attggtctgt ttatgtacga tcatctgggt
    62821 aaacgcacca gcctgccggg atcaactggt ttgcgttttg gcgcaaattc agtgttaaaa
    62881 ccggaaatta agcgcggatt cgaatattcc gactgttggg tagacgatgc ccgtctggtg
    62941 ctcgccaacg cccagatggt ggtgcgtaaa ggcggcgaag tgcttactcg tactcgtgct
    63001 acttctgctc gtcgcgaaaa cggcctgtgg attgtggaag cggaagatat cgataccggc
    63061 aaaaaatata gctggcaagc gcgcggcctg gttaacgcca ccggcccgtg ggtgaaacag
    63121 ttcttcgacg acgggatgca tctgccttcg ccttatggca ttcgcctgat caaaggcagc
    63181 catattgtgg tgccgcgcgt gcatacccaa aagcaggcct acattctgca aaacgaagat
    63241 aaacgtattg tgttcgtgat cccgtggatg gacgagtttt ccatcatcgg caccaccgat
    63301 gtggaataca aaggcgatcc gaaagcagtg aagattgaag agagtgaaat caattacctg
    63361 ctgaaagtgt ataacgcgca ctttaaaaaa cagcttagcc gtgacgatat cgtctggacc
    63421 tactccggtg tgcgtccgct gtgtgatgat gagtccgact cgccgcaggc tattacccgt
    63481 gattacaccc ttgatatcca tgatgaaaac ggcaaagcac cgctgctgtc ggtattcggc
    63541 ggtaagttga ccacctaccg caaactggcg gaacatgcgc tggaaaaact gacgccgtat
    63601 tatcagggca ttggcccggc atggacgaaa gagagcgtgc tgccgggtgg tgccattgaa
    63661 ggcgaccgcg acgattacgc agctcgtctg cgccgtcgct atccattcct ggctgaatcg
    63721 ctggcgcgtc attacgcacg cacttacggc agcaacagcg agctgctgct cggcaatgcg
    63781 ggggcgataa gcgacctcgg ggaggatttc ggtcatgagt tctacgaagc ggaactgaaa
    63841 tacctggtgg atcacgaatg ggtccgccgc accgacgatg ccctgtggcg tcgcacaaaa
    63901 caaggcatgt ggctgaatgc ggatcaacaa tctcgtgtga gtcagtggct ggtggaatat
    63961 acgcagcaga agttgtcgct ggcgtcgtaa ggatgatgcc cggttgaagt gcaaccgggc
    64021 tatttggatt ccttagacta ttcattgtaa ataattgaat aacggcccct gagctaaaat
    64081 cgccaaactt aagcacgtta cccaatcgcc ccacttcttc ctctggtttc tattttttct
    64141 gcaccaatgg caataatatc ccaatgcaaa accaagctgt gcactaatcg ggccgacaaa
    64201 atcagtccaa ggctgcggcc aaaaagggga aaaattcagg ggaggccaga caaacgtcag
    64261 aaagaaattt cggatatttt ccagcaacgc tgatttctct ggagaaatat aaagaatgaa
    64321 attcgagaga attaaccaga aatagaccgg cgaaaagacc agccagcaat gcagtctgtt
    64381 ttttaattca ggaggcgttc tgcgggagaa ttgccaaccg atgccagcca ttacactgaa
    64441 aaaaagtgca tattcaacca ttaactcaag ttgtaattcc tgaagagaaa tacgcacaac
    64501 caggagcgga aaggagaata ttatttctgc cagaaaagtt aacgtgaata taagtacggc
    64561 taagacaata accgtcggcc ataccaataa tggcacgata acaaaaccga gaattacagg
    64621 gggaatataa agccggaatt taatcatttt tattttcctg gtgattcgga tgatgcgtca
    64681 tacatcgcgc ataaacactc atcaattatc cagtcgtcta cccagacctg atgctgtgtg
    64741 ccctgttctg gctgctttgg tgggttctca atcgcctgcc gcgcgcgttc tttcaagatg
    64801 gattgattgt tgaaggccca ggcattgatt tgggtggtaa tgacgttatc gatatcgtcc
    64861 tggttatctc tgatataatc ctcacaatat ctgttcaata aacaggcaag agaaacggtc
    64921 gaccaaaagc cgggaactga attaatggca tccagcaatg gcgcaagagc gatatcggca
    64981 cggaaaaaca tttccagcat atactgtaat tcgacagttt catcgccgga tacccaatca
    65041 gtcatatact ggcaggcaaa tcgttgcaaa attttccatt catcctcacg ccaattttct
    65101 ttactggcaa ggttgatacg gcgcagagaa aaaactacgt gaatattgtc aaaatgcccc
    65161 tgtgcaacaa attccagtaa acgcggcaga aaatagcgga cctcatcgct attttgcgga
    65221 tcaggccctg ggctactatt ccaggcattg attaaggaat acgggatcgc tcgtaacgat
    65281 gtactccgta atgccttttg ttcctgctga ctcaggcaat attcacaaca aaccacaaat
    65341 ttctgcggca ccggataacg acgaaaaata tgataaatct gctcaattaa tgcctgcatg
    65401 gcggggctgg gttgcataat taccccctcg cccgctgatc gcgtgtaaac ggttgataaa
    65461 caaaacgagc attcgccacg ttcaggcgaa taatggcact acgagagagg gaaaagctgg
    65521 ggcgaatgca actattgcag tctcgacagc tttcttcagc gcaattttcc actgtgtaat
    65581 aacgagcgta gagtttcagt tccgcttgct tatcacgaaa tccgcgtaaa ctgttatcgt
    65641 tatgaaattt ccaggcatct ttaaagacct gaaaaatgta gatatcctca atccaggact
    65701 cacatttttt aagcgggcgc tttgctatga tcacccgctc atccgggttt agcatcgccg
    65761 taaattgaac ggtgaggtca tttgtcgtgt tcagatcaca ttgaacatct ggtacatcac
    65821 tgatataggg gtcaaatcca aagtcagcac cacaggcaca aagacggcta atggtggcgg
    65881 tatcgaggga aaatcccggc catacattgc tggacgttaa gccaatattt aatgtgacat
    65941 tatatcgaag caatccctca agaccgtcag caccgacgaa cttttcccct tcatccaggg
    66001 cctggttaat gagttgctga acaatgcctt catcccagta tgccgatttg cagttggcaa
    66061 tcgtaatatc cacgcctgtg aagggatcag taccggcaag gcttgcaaat aatgtattaa
    66121 ctaattccat ttttattatc cagctttaag gtagcgtctt atacatcggg acataaccta
    66181 catcttccac caaactctgt ccctgcggcg ttaaaaacca ctccaacaat ttttgtgtct
    66241 ctgacgtcgt attttcccgc gttaccataa aggcatcgac gatataggga tatttgccgt
    66301 tgcgaatgtt ttcggcagtc ggtgcaatac cgttaatcgc tagcagtttt atatttttat
    66361 cgtcgttcat ttgtgttgcg taataacgga aggtgtaacc tattgcgttg tttgtattac
    66421 ggtattcggc aacgacttta atcattccct ccatcatact tgccacttct gtttcctgcg
    66481 gcgagatcat gcggacattt ttcatgacct gggattgcat caccgtctgg ctaccagagt
    66541 cttccgggcg ttgccaggtc tggatctcct gatcgttacc gccaaccgtg cgccagttgg
    66601 taattgcacc actgaagatg tcacgcactt gttgttcggt cagggaatta accgggttat
    66661 ctgcattgac gatgaaaaca aacgcttcac gggcaaaagg cgtgtaaatc aaagtgacgc
    66721 ccgattcctc cgcgcgtttt ttctgcccac cggaaggttg agccacaaag ataatatcgg
    66781 cattaccttt aacaatcttg ttatatgctt cgggagtacg agagttcgcc agatattccc
    66841 attcgtgaaa atcttccggc aaaacgctta gtgcataaaa ggcagaggca taaatggggt
    66901 acgccgccgt tgcgccatca agtcgcggcc agttttgcgt gaactgaatt tgcggtttac
    66961 cacgcagtgg tgtcagttga ttattgagtt tatcgggtcg ccaggcccag gtatcaatat
    67021 tttcacgcac accgacgccg ggattaagct gtttatataa ataagcctgg taaccggcga
    67081 caaaaccaag catgagaagg ataaacaaga gagaattacg gcaacgtaac ccggacgtgc
    67141 ccgttaaagg atatctggtc ggccagccgt accccagcga aaaactgata tgcgcgccaa
    67201 caggtataat gactaaccac cagtactcac caccgactaa tgaggctaac aaaacgccca
    67261 gccaggggag cataagatca gccaaccaac ccgctcccat ctggcgaaat tgataatctg
    67321 ccagatgcag cgcgattatc cagaccccca gggttaataa tgcgggtact aaaaaaagac
    67381 attgtcgcca gacggctatt tctctttttt ctggtgcact atcgtccgcc cacactcgcc
    67441 ccgagaaaaa tagcattaat gcgcccagcg ccagcataaa aatgttgagc agaaggtaaa
    67501 ttgttggcgt gataaagata ttacaaactt caataatacc ggcaagtccg acacccagca
    67561 tggcaataac cgccgccaaa aactgcgcca gaatggggat gccgaaaaaa gtcattacca
    67621 gcgaggtcaa aatccatttt ctgttttgca ttattctttc cattctttct gaatggcgaa
    67681 attatactcc ccgagtcccc ttgccccttc tggacacttt tccgaaatga tggcgaaaaa
    67741 aacgggaccc tttggccccg ttctatttat tagtgaaatt acaatctcac cggatcaata
    67801 tgccagatat gatcggcgta ctctttgata gtacggtcag aagagaagta gcccatattg
    67861 gcaatgttca gcatcgcttt tgcggtccac tcttcctgaa gctcgtagag ttcatcgact
    67921 ttatcctgac aatcgacata gctgcgataa tccgccagta cctggtagtg atcgccgaag
    67981 ttgatcagcg aatcaaccag atcgcgatag cgacccggat cttccggact gaatacaccg
    68041 ctgccgattt gcgtcagcac ctgatgcagc tcctcatctt tctcgtagta ttcacgcggt
    68101 ttgtagccct gacgacgcag ttcttccact tcttccgctg tgttaccaaa aataaagata
    68161 ttgtcagcac cgacatgatc cagcatctcg acattcgcac cgtccaacgt accgatagtc
    68221 agcgcaccgt taagcgcaaa cttcatgtta ctggtgccgg aagcttccgt ccccgccagc
    68281 gaaatctgtt cagacagatc ggccgccgga atgatcaact gcgccaggct aacgctgtag
    68341 ttcgggatga acacgacttt cagcttgtcg ccaatctgcg gatcgttgtt gatcactttc
    68401 gctacgtcat tgatcaaatg aataatgtgc ttcgccatgt aataggccga agccgcctta
    68461 ccgccaaaaa tattcacgcg cggtacccac ttcgcatccg ggtcggcctt gatgcggtta
    68521 tagcgggtaa tcacatgcaa cacattcatc aattgacgtt tgtattcgtg aatacgtttg
    68581 atttgtacat cgaacaacgc ctttggattc accaccacat tcagctgctg ggcgatatac
    68641 tctgccagac gctttttgtt ctccagcttc gcctgatgca cagcgtgatt aaccattggg
    68701 aaatcacagt gttgttgcag ctcattaagc aggctaaggt cggtgcgcca gttacggccc
    68761 aggtgttcgt ccagcacggc tgaaagcgat gggttcgcta ccgctagcca gcgacgcggc
    68821 gtcacaccgt tggtgacgtt ggtgaaacga cccgggaaga ttttcgcaaa gtcggcaaac
    68881 aacgattgca ccatcagatt agagtgcagt tccgatacac cgttaacttt gtggctcaca
    68941 acaaccgcca gccaggccat acgcacacga cgaccgttgg attcatcaat gatcgacgcc
    69001 cgtcccagca gatcggtatc gttcggatac tgttcctgca aggttttcag gaaatagtcg
    69061 ttgatttcaa agatgatctg caggtgacgc ggcagaattt tacccagcat atcaaccggc
    69121 caggtttcca gcgcctcgct catcagcgtg tggttagtgt aggagaagac ctgacaacac
    69181 acctcaaacg cgtcgtccca gctaaattgg tgctcatcga tcagcagacg catcatctca
    69241 ggaatcgaca gtaccggatg ggtatcattg agatgaatcg cgattttatc cgccaggtta
    69301 tcgtaggttt tatgcaactg ataatggcgg cttaaaatgt cctgaatggt cgaggaaacc
    69361 aggaagtatt cctgacgcag gcgcagctca cgcccggagt aggtggagtc atccggatac
    69421 agtacgcgag atacgttctc ggagtggttt ttatcttcca ctgccgcgaa gtagtcaccc
    69481 tggttgaatt taccgaggtt aatttcgcta ctggcttgcg cactccacaa acgcaacgtg
    69541 ttggtcgcgt cggtatcgta accagggatt atctgatcgt aagcgactcc cagaatctct
    69601 tcggtttcaa tccagcgcgt ttttttacct tcctgctgaa tgcgaccacc aaaacggact
    69661 ttatagcgcg tgttgtggcg tttgaattcc cacgggttac cgtattccag ccagtagtct
    69721 ggcgactctt tctggctacc gttaacgatg ttctgcttga acataccgta gtcatagcgg
    69781 atgccgtaac cgcgccccgg caaccctaac gtcgccagag aatcaaggaa gcaagccgcc
    69841 agacgtccca ggccaccgtt accgaggccc ggatcatttt cttcatcaat cagctcttcg
    69901 agatttaacc ccatcgcttc cagtgcgccc tgtacatctt cgtaaattcc tagcgacaac
    69961 atggcgttgg agagcgtacg gccaatcaaa aactccatcg acaggtagta aacctgacga
    70021 gtttcttgcg acaactgggc acggtttgaa cgtaaccagc gctccacgag acgatcgcgc
    70081 acagcaaata acgttgcgtt cagccattca tgtttattgg cgacgaccgg gtcctttcca
    70141 atcgtaaaca tcagcttgta agcgatagag tgcttaagag cttctacgct aagcgtgggc
    70201 gatgaatatg taaacggagc attcatatag gcgtttcctg aaaactattt caagcgatag
    70261 taaagctcac ggtacgactt cgccgcgacc tgccagctaa aatccattgc catagcctga
    70321 cgttgcacaa accgccacag tgaaggacgg gaccacagta caaaagcacg ccgaatagcc
    70381 cgtaacagcg accaggcatt actatcttca aagacaaacc cactggcgac gccatctgca
    70441 aggttctcaa gagaacagtc agaaaccgta tcagcaagcc caccggtgcg ccgcactaac
    70501 ggcagcgtac cgtacttcaa tccataaagt tgcgttaagc cgcacggttc aaaacggctg
    70561 ggcaccagaa tgacgtccgc gccgcccata atgcgatgcg aaaatgcttc gtgatagcca
    70621 atctgaacgc ccacctgacc ggggtattcc gctgccgccg caaggaaacc ttcctgcagc
    70681 accggatcgc ccgcgccgag tagcgccagc tgcccgccct gctccagaag gcccggtaag
    70741 gcttccagca ccaggtcgag acctttctgg ctggtcagac ggctcaccac tgcaaaaagc
    70801 ggcactttat cgtcaacctt aagccccatt gcgatttgta actggcgctt attttccgct
    70861 ttatcttcca acgtatcgcg ggtgtaacgc gaggccaaca gtaagtccgt ctcaggactc
    70921 cagattttct cgtccacgcc gttcagtacg ccggaaagac gcccttcacg gtgacgctgt
    70981 tgcaacagac cttccatacc gtaggcaaac tgcggttcgg tgatctcgcg agcgtaggtt
    71041 ggactgaccg ccgtaatgtg atcggcatag tacagaccgg ccttcaggaa agagatttgt
    71101 ccgttgaatt ccagcccatg aatattaaag aatgaccatg gcaattggat gtcattcatg
    71161 tgatgtgcat aaaacatgcc ttgataggcc aagttgtgta cagtaaacac cgacttcgcc
    71221 ggacgcccgc gcgccgccag atacgcaggc gcaaggcctg catgccagtc gtgcgcatgc
    71281 accacatcag gacgccagaa tgggtcaagc ccgctggcca tttctgcccc aacccacccc
    71341 agcagcgcaa aacgcaatac gttgtcggta taggcaaata agttggtatc gtgatacggg
    71401 cttcccggac gatcatagag atgcggcgcg tcaatcaggt atatgccaac cccgttgtaa
    71461 tgaccgaaca acagcgtgat atgtccggcg aaggtatcac gacgggatac tacctgcgca
    71521 tcggtcacgc cacggcgaat atcgggaaat gcaggcaaca gtacgcgagc gtcaacgccg
    71581 tctgcgattt gtgctgcggg taatgcccca ataacatcag ccagaccgcc ggttttaagc
    71641 agcgggaaca tctctgaaca tacatgtaaa acctgcatta tcgctcctgt ttatgcccta
    71701 acttccgtag catttcgcgc gttaccagca cgatgccttc ttctgaacga tagaaacgac
    71761 gtgcatcttc ctctgcgttt tcaccaatca ccatgccttc cggaataaca caagcacgat
    71821 cgatgacgca gcggcgcaga cggcacgagc gacctaccca tacttccggt aacaatacgg
    71881 cggaatcaat gttgcagaat gaattcacgc gaacgcgcga gaacagaacg gactgcacca
    71941 ccaccgaacc ggagatcaca cagccgccgg ataccagtga gttaagggtc atcccgtggc
    72001 taccggagcg atcctgcacg aatttcgctg gcggtaatga ttcattgtag gtgcgaattg
    72061 gccaattgcg atcgtacata tccagttccg gcaccacaga ggccagatcg aggttcgctt
    72121 tccagtaagc ttccagcgta cccacatcgc gccagtacgg ctcggcatcc gggtcggatt
    72181 gtacgcaaga gagcgggaac gggtgcgcat aggccagacc ggcttcggtg atcttgggaa
    72241 tcaaatcttt gccaaagtcg tggctggagt tctcatcacg atcgtcttct tccagcagtt
    72301 catacagata gtcggcgtca aagacgtaga tacccatact cgccagagat ttgctcggat
    72361 cgttcggcat tgacggtggg ttagcaggtt tttcaacgaa ttcgataatt ttatcgttct
    72421 catcaaccgc cataacgcca aatgcggagg cttcttcaat cggtactggc atacaagcaa
    72481 cggtgcaacg tgcgcctttt tcgacgtgat cgataagcat acgcgagtaa tcttgcttgt
    72541 agatatggtc gcccgccagg atcaccacgt attccgcttt ataacggcgg ataatatcga
    72601 ggttttgggt gaccgcatct gcggtgccgc gataccagtt ttcccctttc attctctgct
    72661 gcgctggcag cagatcgaca aactcgttca tttcttcatt aaagaatgac cagccgcgct
    72721 gaatgtgctg caccagagtg tgggactggt actgggtgat cacgcccata cgacggatcc
    72781 cggagttgat gcagttagac agcgcaaagt cgataatgcg gaacttaccg ccgaagtgta
    72841 cggccggttt tgctcgctta ttggttaaat ccttcaggcg ggtaccacgt cctcccgcca
    72901 gtatcagggc aacagatttc aatggcagct ggcgcgccaa cattaagtga tcgttcttct
    72961 ctaaactaac catgactaac tcctttttta tcatctctgg aacacacaca atccgtgtgc
    73021 aggtccctgc cagacagccg taatcactgg gttatcctct ccagcgaatg ggggaatggc
    73081 gtgccactcc ccagcaggta aaacaatctc tgttacctca agcgtggcgt taattgcgat
    73141 caaaaagcga tccgagagca gaatttgcag ctgtttcggc ccgttttgcc actcatccgt
    73201 gcttaaaggt tgagcatatc gatttagcca acggacattg ccgtcgcctt cttcccacca
    73261 gcgattctcc accaaagcgg gaatacgctt gcgcagatgg attaacgcgg cggtaaatgc
    73321 ggttaaacca ctgcttgcct gcgaccagtc caaccaggtt aattggttat cctgacagta
    73381 ggcattgtta ttgccatgct ggctgtgacc atgttcgtca ccggccagta acatcggcgt
    73441 gccctgggag agcaacaacg ttgttaacag ggcatgaatg ctgtcgcgcc gccgttcaac
    73501 cagatcaaga gtaccgccta acccttcttt accatgattg ttactgtaat tgttgttggt
    73561 cccgtcgcga ttttcttctc cgtttgcttc attgtgttta tggttgaagc aaacgcagtc
    73621 gcgaagcgta aaaccgtcat gcgcggtgac gagattaatc gcggcactcg gcagacgacc
    73681 attacgttta aaaacatcgc tggaggcagc aaaacgcccg gcaaacgccc ccagaggcaa
    73741 atcataatgt agccagaaac gacgggcagc atcgcggaaa tgatcgttcc actcggcaaa
    73801 cagcggcggg aaatttccca cctgataacc accaggagcg atatcccacg gttcagcaat
    73861 taacttcacc tgcgagagca ccgggcagtt ctggatagcg gtaaacaacg gcgcatcctg
    73921 acggaactct ggcgtacggc ccatgactgc cgccagatca aagcggaaac catcgacgtg
    73981 acaggtttct acccaataac gcaggcaggc gcaggcataa tccaccaccg ccggatgact
    74041 caaattgagc gtgttgccac aaccggtcca gttgtgataa tcgccgtctt ctcttatcca
    74101 ataatagcta cggttgtcga tcccacgcag cgagaataac gggccgtcga ggtccagttc
    74161 cgcactatgg ttgagcacga tatcaagaat gacttcgata cccgctttat gcagtgcttt
    74221 gattgcatcg cgaaactcat gcagcgccgt ttctggcgag caggcatacg ccggatgcag
    74281 cgcaaacatc gccaccgggt tgtaacccca gtagttactt agccccatgc gttgcagacg
    74341 tggttcactg gcaaactgcg ccactggcag cagttccagc gcggtaatgc ccaattgttt
    74401 caaatagttg atcatcaccg gatgtcccag ggctttataa gtgccacgga tctcgaccgg
    74461 gatctccggg tgcaggtacg ttaatccttt aacatgggct tcataaatga tggtgctgcc
    74521 ccacggcgtg cgcggcgggg catcatcttc ccagtcatag tgatcaacca ccactacgca
    74581 tttcggcgca atggcggcgt tgtcgcgata gtcaggttca ttatgaccgg cgtgcagcag
    74641 cgggttatct ttgaattccc cgtcaatttg ccgcgcgcaa ggatcaatca acaacttcgc
    74701 cgggttaaag cgatgcccct cggcgggttg ccaggggcca tgaacgcgat aaccataacg
    74761 caaacccggg cgcgcatccg gcagataacc gtgccaaatg tcgccactgt gccctggcaa
    74821 gtcatagcga tgttcctggc cattggcgtc aaagacacac agctctaccc gctcggcatg
    74881 agcggagaaa agtgtgaagt tgacgccctg accgtcgtaa tgcgcgccga ggggagcggg
    74941 tttgccaatg gcgagttgtg tcattctgcc tcccgaacca gccagatagt ggccagcggt
    75001 ggtagcgtca ggcttagtga atgctgacga ccgtggctgg caatctcatc gctgtgtacc
    75061 gtgccgccat tgcctgcatt actgccgtga tagtgcatgg aatcggtatt gaggatttca
    75121 cgccatttgc ccggctggtt tatgccgaag cgataatcat gacgcggtac cggcgtaaag
    75181 ttactggcaa cgatgatttc gttaccctct ttatcgcgac gcacaaagat cagcaccgag
    75241 cgttctttgt catccaccac cagccattca aagccgtacg ggtcaaaatc cagttcatgc
    75301 attgctttat ggtggcggta ggtgaggttc agatcgcgca ccagacgctg gacaccgtgg
    75361 tgccagttat cgccgccttc caacagatgc cagtcgaggc tggcgtcatg gttccactcg
    75421 cggccctggg caaattcgtt acccatgaac agtagtttct tgcccgggaa tgcccacatc
    75481 cagccatagt aggcgcgcag gttcgcgaat ttctgccatg cgtcgcccgg catgcggtcg
    75541 agaatcgatt ttttaccgtg gaccacttca tcatgcgaca acggcaggac gaagttttca
    75601 gtgtagttgt agagaatccc gaaggtcagt ttatcgtgat gatactgacg ataaaccggg
    75661 tcgagcttca tgtagtccag ggtgtcatgc atccagccga ggttccactt gtaccagaag
    75721 cccagaccgc ccatatcctg cggacgagaa acgccaggga aatcggtaga ctcctcagcc
    75781 attgtcaccg caccggaaac ctgctcacca agaatacggt tggtattacg caagaattca
    75841 atcgcttcaa gattctcgcg cccgccaaat tcgttcggga tccactcccc ctctttacgg
    75901 ctgtagtcgc gataaatcat tgacgccacc gcatcgacgc gcagcgcatc aataccaaaa
    75961 cgttcaatcc agtaaagcgc gttaccgacg aggaagttac tgacttcacg gcgaccatag
    76021 ttgtagatca gcgtgttcca gtcctgatga tagccttcac gcggatcgct gtgttcatac
    76081 aagttcgtgc catcaaattc ggcaagcgca aagtcatcgg tcgggaagtg gcctggcacc
    76141 cagtcgagaa tcacgttcag accagctgcg tgtgcggcat caatgaaata acggaagtcg
    76201 tcgcgagtac caaaacggcg ggttggtgca tacaggccgg ttggctgata accccaactg
    76261 ccatcgaagg gatgctcgtt aatgggcagt agttcgaggt gggtaaagcc catccattta
    76321 gcataaggca ccagttgatc ggccagctcg cggtagctca accagaaatt gttgtcggtg
    76381 tggcgacgcc aggaacccag gtgaacttca taaatagaga ttggcgcatc aaactgattc
    76441 gcttttttgc gctcttcagt ctgtacaacc ttttccggca gcccgcaaat aagagacgcg
    76501 gtttccgggc gcatttgcgc ttcgaaggca taagggtcgg acttcagacg caagttgcca
    76561 ttggcatcaa tcatctcgta tttatagagc tgaccgttat gcgccccagg gataaacagt
    76621 tcccagatgc cgctctcttt acgcaggcgc atcgggtgac ggcgaccgtc ccagtagttg
    76681 aattgcccaa ccaccgagac ccgacgggcg tttggagccc agacagagaa acgcgtacct
    76741 gtgacgccat ccatagtatc tgcatgcgcg cctaaggttt catacgggcg caggtgagta
    76801 ccttcagata atagccaggc atccatttcc tggattagcg gaccaaaacg gtaaggatca
    76861 tcaatcaggt tttgctgacc atgccagaca acagccaact gatagcggaa aaaattctta
    76921 cgtcgcggaa tgacgccgct aaagaatccc cgtgagtcga gacactccag ttttgcgagt
    76981 ttgcgcccgg ttttcggttc aatcacccac acatcggtag cgtcgggtaa aagggcacgg
    77041 acttccagtc ccgcggtggt tttatgcatt cccagtacgg aaaaaggatc cgcaaaatgg
    77101 cctgcaatta gcgcgttaat cacgtctcta tcgatacgat cggacatgct tgtcttcctg
    77161 ttttattgtg tcaccccatc caattggatt tttgacttct ggttgtgaca tttttttgac
    77221 ctgaacggcg cagcactctg tgcatcctct ctgcgtcgtc ctcacttcag gtaaggctgt
    77281 gaatactcat gtattcagcc acccttaaag aatagccaat gctctattta actcccggta
    77341 aatcatgaaa catctgcgct tactcctgta ttacgcacta acaggggcgg catcgcgccc
    77401 cagatttaat gaataaagat tacgccagtt gacgaagcat ccgacgcagc ggctccgcgg
    77461 ccccccacag cagctggtcg cccactgtaa aggctgacag gaactctggt cccatattca
    77521 gcttacgcag gcggcctacc ggcgtggtca gcgtgccggt aacggcagct ggggttagct
    77581 cacgcatagt gatttcccga tcgttcggaa cgactttcgc ccacggattg tgcgcagcca
    77641 gcagttcttc cacggtcgga atagacacat cttttttcaa tttaatagtg aatgcctggc
    77701 tgtggcagcg caatgccccg acacgcacac ataaaccatc taccggaatt acggaagatg
    77761 tgttgaggat cttgttggtt tccgcctgcc ctttccactc ttcgcggctc tgaccgttat
    77821 cgagctgttt gtcgatccac ggaatcaggc tacccgccag cggcacgcca aagttatcca
    77881 ccggcagctc accgctacgg gttaaggttg tgactttgcg ttcgatatcg agaatagcag
    77941 aggacggggt cgcgagttca tctgccacat ggccatacag atggcccatc tgggttaata
    78001 actcacgcat atgtcgcgca ccaccgccgg aagcggcctg gtaggttgca acggacaccc
    78061 aatcaacaag atcattggcg aataaaccac ccaacgacat caacatcagg cttacggtac
    78121 agttaccgcc aacaaaagtc ctgatgccat tatttaatcc gtcggtaatg acgtcctgat
    78181 tgacggggtc aagaatgatg atggcgtcat ctttcatgcg cagagacgat gctgcgtcaa
    78241 tccagtaacc ttgccatccg ctttcacgaa gctttggata gatttcgttg gtataatcgc
    78301 cgccctgaca ggtcacaatg atatcgaggg cctttagcgc ctccagatca aaggcatcct
    78361 gaagtgtgcc agtggttccg ccaaaagacg gcgcagcctg gccaagctga gaagtagaaa
    78421 agaagacagg gcgaatggcg tcgaagtcgc gctcttcaac catgcgttgc atgagaacgg
    78481 agccgaccat accgcgccag ccgataaaac caacattttt cataagcgtt ttttcctgca
    78541 aagatgtgtg ctgtataaat gtgccggtct cctcttggca catctttcac catacaaaaa
    78601 gcagccaaag tcgcaagtga aattaatcaa tgatagcgaa gccatcagta atgcgactta
    78661 tcctgctttg ttagcacgca gaaagtccgc ggcaattatc agggaatttg agttatgaat
    78721 gaaatcattt ctgcagcagt tttattgatc ctgattatgg atccgctcgg aaacctacct
    78781 attttcatgt ccgtactgaa acatactgaa ccgaaaagac ggcgggcaat catggtgcga
    78841 gagttgctta ttgctctcct ggtgatgctg gtgttcctgt ttgcgggtga gaaaattctg
    78901 gcatttctca gcctgcgagc agaaaccgtc tccatttctg gcggcatcat tctgtttctg
    78961 atcgccatta agatgatttt ccccagcgct tcaggaaata gcagcgggct tcctgcaggt
    79021 gaagagccat ttatcgtgcc gttggcaatt ccgttagtcg ccgggccgac tattctcgcc
    79081 acgctgatgt tgttgtctca tcagtacccg aatcagatgg ggcatctggt gattgctctg
    79141 ctgctggcct ggggcggcac ctttgtcatc ctgctacagt cttcgctatt tttacgtctg
    79201 ctgggcgaga aaggggtgaa cgcacttgaa cgcctgatgg gattgattct ggtgatgatg
    79261 gcaacccaga tgttcctcga cggcattcga atgtggatga aggggtaacg aggagagttt
    79321 ttcgaaaaga acccggtagc gtcacaacta ccgggcgaac aaacttaact caacagctgg
    79381 aacgcaatca tcccaacgat ggcaccgaca gtgccgagga tggtttccat catggtccag
    79441 gttttcagcg tttcggcttc ggtcgcgccg gtaaatttac cgaacaacca gaaaccagcg
    79501 tcgttaacgt ggctgacaac aatcgaacca ccagcgatac aaatcgacag tgccgccatt
    79561 tgcgcaccag agtagttcag ttgttcaatg actggcatca ccagtcccac cgccgttaaa
    79621 caggctacgg tggcagaccc ctgaatgatg cgcactgcag cggccagcac gaagcaggtg
    79681 atagcaatcg gcaggcccat gccggttaac gcttcgccca gtgccggacc tacgccagag
    79741 tcaaccagca cctgtttgaa cacgccgccc gcaccaatca ccagcagaat gatccccgcc
    79801 ggttgcagcg cgtgaccgca aatctccatc actttgtctt ttggcatgcc ctgacgcatc
    79861 gccaggccat aaatcgccac cagacaagca accagaatcg cggtaaacgg atgaccaata
    79921 aactcgaacc attcgtaagc ggtagagcct tctggcacaa aacgcgcggc aatggttttc
    79981 agccccacca gcaccagcgg caacaggatc agcgacaggc tgaatccgaa ggacggcatt
    80041 ttgccttcgc cgagatgcgg ttcgctgatg tcgtcaggaa tatgcaactc cacgtaacgg
    80101 ctgatgaaat taccccacag cggcccggca ataatcattc ccggaattgc cgcacacagg
    80161 ccaatcagga tcatccagcc aaaatcggcg ttcatctgcg atgccagcag cattggcgct
    80221 ggccctggca ccaggaacgc cgccgctgcc gccacgcctg cgaataatgg gattaccagc
    80281 ttcaccaggt tcgtaccggt gtggcgcgcc attgagaaag caacgctaat cagcagaaca
    80341 atcgccactt caaagaacag cggtagcgca cagaccagcc ccgcaaggcc gatggcataa
    80401 tgcgcgcggc tgtgaccgaa ggatttgagc attttgacgg caatctgatc gactgcgccg
    80461 gtttcatgta agatcttgcc aaacatagct cccagggcga caaccaccgc caggaagccg
    80521 agggtgcctc ccatcccttt ttccatcgtc gctgcgattt tatcgagcgg cataccggaa
    80581 aaaaggccag cccccatgga caccaccatt aaagccagga aagcgtgcat acgcgccttc
    80641 atgactaaaa acagcagcag taaaacagac cctactgctg ttaaaacaag cgttaatgta
    80701 gtcactactt atttgccttt tttaataacc tcaatggtgc ttgccacaac accttccagc
    80761 ggttgatcga tatccaccac cagtacatcg gtttcgtccg cacccggctc ctgcagcgtt
    80821 tcaaactgcg tcaccaacat ttgggtttta aagaaatggc ctttgcgcgc tttcaggcgg
    80881 ctttcaatca catcaaaatc gcctttcaaa tagatgaaag agagattcgg attaccttca
    80941 cgcagcaagt cgcgatagtg ttttttcaat gcagaacaga cgatcagcga cactttatta
    81001 gtgcgctgca tagcaaacgc ggcgtcgttc agcgcctgca accacggttt gcgatcgtcg
    81061 tcattcagtg gttcgccaga cgccattttt tcgatattgc gccgtggatg gaggaaatcg
    81121 ccatcaagaa acgcggcatg aagttgatgc gccacttcac tggcgaccgc agatttgccg
    81181 ctgcccgata cgcccatcaa gacgtaaatg tggtgatcat ggttagtcgt gctcaaagtg
    81241 gtgcccccac aatacaagaa ttaaaaatgt tacgggtaac tgttatcggt aacattgtcc
    81301 agccggacaa tatcagaagc aatatccatc catgccttaa gtgtataagt gtgagctact
    81361 tcaaatttgt gggcttaaat agatccgccc ggtgacaagg tgaaacctaa atctaacatt
    81421 ttcggtgtca cagattcgcc acgaatacgc gccagcaggc gttcagcgcc aatactgccc
    81481 atccgctcac gcggcgtcag cacgctcgca agtcgtggct ccatcacctg accaatgtca
    81541 tgaccgtgga aaccggcaat cgccatatcg tcaggaactt ttaaccccag acgctgacat
    81601 tcaaacgccg cgccgaccgc caggtcatca ttcgtacaga acacgccatc cagctgcgga
    81661 tattcccgcc gcgcctggcg aatcagttca ataccggaag agtaagaaga agattgctca
    81721 accatcacgc tatatggcac caggcctgca tccagcatcg cctgttcgta tcccttctgt
    81781 ttgatgatag tacgttcgtc gagacgtgcg ccgagatagg caatgtggcg atgcccgcga
    81841 gcaataatgg cagtggtcat ctggcgtgct gcttcaaagt tatcaaaacc gacggcgata
    81901 tcaaggcatg gcgacttgct gtccatcagt tccaccacgg gaatacccgc cacttcaatc
    81961 atctttaagg tgcgcggcgt gtgggtacgt tcggtgagga tcaggccgtc gatattccag
    82021 gagagcatgg attcgaggcg ttcttgctcc atttccggtt tataaccgta gtgcgccagc
    82081 atggtctgat aaccgtgcgc gtcggtgacg ctttcgattc cgcgtaatac ttccgcgaaa
    82141 acctggttgg tgagagaagg taacaggacg ccaatcgccc ggctggtggc gttagagagg
    82201 atatcgggcg cacgattggg aatatagccc agttcatcaa gagccgcggc aatcttgccg
    82261 cgtagagcga cggaaacctg ctccgggttg cgtaaaaaac ggctgaccgt cattttggtc
    82321 acgcctacac ggtcagccac atcctgaagt acgggtcttt tctttttcat cgtcctgaag
    82381 gtacaaaaga gatagattct cttagtttaa cacggacaca tcagaacctt cccggggaaa
    82441 acagtgcttg tttacaatta ttagatatac actaaatcaa ttgcatcgca ttgtgctaac
    82501 gacgcggcaa aaccccggaa gcgtacatca gcacgcgacc ggagcgagcg aggaagcctg
    82561 caaaaaggca ggcttcaaag atgccgttga gttttaaacc ggcggcagat cgaacagtaa
    82621 cacttcgctg tcgctatccg catggatgga gattgcctgc tcatcccaga ttgccagacc
    82681 atcgctggtc gaggctttca cgccgttaat ggtgacattg cctttcacca cctggatcca
    82741 gacgcggcgt tcagcggcaa tctgatgcac cgactgctca tctttcagca acgcccagcg
    82801 gtacagttcc atatcctgat gcactttcaa tgaaccatct cgcgcatccg gcgagagcac
    82861 cagctgtttg ccctgtacgg catcgaagcg acgctgttca taacgcggcg taataccgtt
    82921 ttcttcaggc atgatccaga tctgatacag atgcagacgc tcggtgctgc ttgggttgta
    82981 ctctgagtga cgaatacccg taccagcact cataatctgg aactcacccg ccggaacctg
    83041 ctctttattg cccatgctgt cctggtgctc aacagtacct tccagcacgt aggtcaaaat
    83101 ttccatatct ttatgtgggt gagtgccgaa gccctgccct gcttcaatca cgtcgtcgtt
    83161 aatcacgcgc agcgcggaga agcccataaa gttcggatcg taatagttgg caaaagagaa
    83221 agtatgccag gagtccagcc agccatgatt tgcatgacca cgttcatttg ctttgcgtaa
    83281 gtagatcatt tgtattcgcc ccctgaatga tttcgatggg cttagtgtgg acccgatctg
    83341 cctgggatga tagagggtga aaattgaccc ctctgttcaa aaaaattgaa ctattcgagg
    83401 ggccatcaaa cttacttcgc gagagtcact gtggagggag aggcttgctc gaatccgcgt
    83461 tcaaggattt ccagattcgt aagaacttca gattccttga cgtaatttgg cgcaccgtgg
    83521 gtgatggttt gatacaacgc atcataaacg cgcccgtaat cgcccatctc cggcttaatc
    83581 tcttctctga ccgtcacgcc ctcgtcattg acatactcca gcacaccgac cgaatcatcc
    83641 gctgagaatc ccggttcgcc cggcataata ttagccttca ggctggtttc ctgctggtcg
    83701 ataccatatt taataaacga acctttctta ccgtgaacga taaatttcgg ataatcgatt
    83761 ttcaccagat ggctggtttt gacgatggct tttagatcgc cataaaacag ttgcgcttcg
    83821 aaagtatcgt caggattggc tttattgcgc aggctgcgga tgtcataagc gacgtgatcc
    83881 gggcgaccga acagagaaat aatctggtcc atcgtatgca caccaaggcc atagaacgcg
    83941 ccatcctgcg gcaacccagg tttggtttct gcgaccgggc ggtaatagtc aaaatggctt
    84001 tccacttcaa caatctctcc caacttgcca ctttcaatcg cttttttcgc tgtcaggaag
    84061 caggagtcaa agcgacgatt ctgatacggc gtgacggtca gccctttgct tttcgtcaac
    84121 gcaaacagct ccttcgcctg ggcaagcgtc ggggtgaacg gtttttcgac cagcacattt
    84181 ttcccggctt ccagcgcgcg tttcgcgtac tcgaaatggc tgtccgcgtg ggtgcagaca
    84241 acaaccagct taacatcggg atcgtttagt acttcgtcga gatcgctggt gaaatggata
    84301 tgggaataaa tgggagcctg ttcttccggc ttcgcatggc gacgaaaaat atgcgcgaca
    84361 tgccagctat ccttgcggtt aagtacatac ggcagatggt aacgggtggt gcttttgccg
    84421 aagccaataa aggcgcagtt gatgaccatg atttcgtcct ttttaaggtg gttatttaca
    84481 ccttagcgca aagcggacgt ggttcctact ggagtgcgca taacgaacac aagcactccc
    84541 gtggataaat tgagaacgaa agatcaaaaa aaaagccagc acccggctgg ctaagtaata
    84601 ctggaagcaa tgtgagcaat gtcgtgcttt caggttctcc gcgagggtct tcctgatcgc
    84661 gagacaataa taatcattct cattcgcact tgtccaacac tttttgcaaa aaaatgcatt
    84721 tgactcgcat tcgaaagtca atgatgttga aagggacatt taccccaaag aggacaaagg
    84781 aatgagtgag atagtgatac gccatgctga aacgcgcgat tacgaggcca tcaggcagat
    84841 tcacgcccag ccggaggtgt attacaacac gctacaagtg cctcatcctt ccgattatat
    84901 gtggcaggag cgactcgccg atcgtcccgg catcaagcaa ctcgtcgcct gtattgatgg
    84961 agacgtcgtg ggccatctca ccattgacgt gcaacagcgc ccacgccgca gtcatgttgc
    85021 cgattttggt atctgtgtcg actcccattg gaagaatcgc ggcgtcgcca gcgcccttat
    85081 gcgagagatg attgaaatgt gcgacaactg gttgcgggta gatcgcattg aactaaccgt
    85141 gtttgtcgat aacgccccgg caattaaggt ctataaaaaa tacggctttg aaattgaagg
    85201 gactggtaag aagtacgcat tgcgtaatgg tgaatatgtc gatgcgtatt atatggcgcg
    85261 ggtgaagtaa gatagtgccc tttttctgag atgggaaaag ggtgtcattc aaaatcggca
    85321 tacc
//
LOCUS       DAJOSY010000004        80820 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJOSY010000004 DAJOSY010000000
VERSION     DAJOSY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.004"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            203..697
                     /gene="napF"
                     /locus_tag="OHN27_000279"
     CDS             203..697
                     /gene="napF"
                     /locus_tag="OHN27_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCQ2284789.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            687..950
                     /gene="napD"
                     /locus_tag="OHN27_000280"
     CDS             687..950
                     /gene="napD"
                     /locus_tag="OHN27_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCQ2284790.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            947..3433
                     /gene="napA"
                     /locus_tag="OHN27_000281"
     CDS             947..3433
                     /gene="napA"
                     /locus_tag="OHN27_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HCQ2284791.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3440..4135
                     /gene="napG"
                     /locus_tag="OHN27_000282"
     CDS             3440..4135
                     /gene="napG"
                     /locus_tag="OHN27_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCQ2284792.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4122..4985
                     /gene="napH"
                     /locus_tag="OHN27_000283"
     CDS             4122..4985
                     /gene="napH"
                     /locus_tag="OHN27_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCQ2284793.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4982..5431
                     /gene="napB"
                     /locus_tag="OHN27_000284"
     CDS             4982..5431
                     /gene="napB"
                     /locus_tag="OHN27_000284"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCQ2284794.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5441..6043
                     /gene="napC"
                     /locus_tag="OHN27_000285"
     CDS             5441..6043
                     /gene="napC"
                     /locus_tag="OHN27_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCQ2284795.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6062..6679
                     /gene="ccmA"
                     /locus_tag="OHN27_000286"
     CDS             6062..6679
                     /gene="ccmA"
                     /locus_tag="OHN27_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCQ2284796.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQSMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            6676..7338
                     /gene="ccmB"
                     /locus_tag="OHN27_000287"
     CDS             6676..7338
                     /gene="ccmB"
                     /locus_tag="OHN27_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCQ2284797.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7380..8117
                     /gene="ccmC"
                     /locus_tag="OHN27_000288"
     CDS             7380..8117
                     /gene="ccmC"
                     /locus_tag="OHN27_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCQ2284798.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8114..8323
                     /gene="ccmD"
                     /locus_tag="OHN27_000289"
     CDS             8114..8323
                     /gene="ccmD"
                     /locus_tag="OHN27_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCQ2284799.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8320..8799
                     /gene="ccmE"
                     /locus_tag="OHN27_000290"
     CDS             8320..8799
                     /gene="ccmE"
                     /locus_tag="OHN27_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCQ2284800.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8796..10739
                     /gene="ccmF"
                     /locus_tag="OHN27_000291"
     CDS             8796..10739
                     /gene="ccmF"
                     /locus_tag="OHN27_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCQ2284801.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10736..11293
                     /gene="dsbE"
                     /locus_tag="OHN27_000292"
     CDS             10736..11293
                     /gene="dsbE"
                     /locus_tag="OHN27_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCQ2284802.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11290..12342
                     /gene="ccmH"
                     /locus_tag="OHN27_000293"
     CDS             11290..12342
                     /gene="ccmH"
                     /locus_tag="OHN27_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCQ2284803.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYPTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12377..13024)
                     /gene="narP"
                     /locus_tag="OHN27_000294"
     CDS             complement(12377..13024)
                     /gene="narP"
                     /locus_tag="OHN27_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCQ2284804.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13344..15935
                     /gene="yejO"
                     /locus_tag="OHN27_000295"
     CDS             13344..15935
                     /gene="yejO"
                     /locus_tag="OHN27_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCQ2284805.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEIKGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEWG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIVNNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLENGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLIFGYSHSDIGFDRGGKGNIDSYTLGDYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAYVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNHVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            complement(16039..16115)
                     /locus_tag="OHN27_000296"
     tRNA            complement(16039..16115)
                     /locus_tag="OHN27_000296"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16079..16081),aa:Pro,seq:ggg)
     gene            complement(16190..17950)
                     /gene="pbgA"
                     /locus_tag="OHN27_000297"
     CDS             complement(16190..17950)
                     /gene="pbgA"
                     /locus_tag="OHN27_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCQ2284806.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(17970..18197)
                     /locus_tag="OHN27_000298"
     CDS             complement(17970..18197)
                     /locus_tag="OHN27_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCQ2284807.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            18379..19386
                     /gene="yejK"
                     /locus_tag="OHN27_000299"
     CDS             18379..19386
                     /gene="yejK"
                     /locus_tag="OHN27_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCQ2284808.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(19525..19809)
                     /gene="rplY"
                     /locus_tag="OHN27_000300"
     CDS             complement(19525..19809)
                     /gene="rplY"
                     /locus_tag="OHN27_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCQ2284809.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(19934..21694)
                     /locus_tag="OHN27_000301"
     CDS             complement(19934..21694)
                     /locus_tag="OHN27_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCQ2284810.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPVCGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            21844..22539
                     /gene="rsuA"
                     /locus_tag="OHN27_000302"
     CDS             21844..22539
                     /gene="rsuA"
                     /locus_tag="OHN27_000302"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCQ2284811.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            22567..23757
                     /gene="bcr"
                     /locus_tag="OHN27_000303"
     CDS             22567..23757
                     /gene="bcr"
                     /locus_tag="OHN27_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCQ2284812.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            24090..24434
                     /locus_tag="OHN27_000304"
     CDS             24090..24434
                     /locus_tag="OHN27_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2284813.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(24438..26027)
                     /gene="yejF"
                     /locus_tag="OHN27_000305"
     CDS             complement(24438..26027)
                     /gene="yejF"
                     /locus_tag="OHN27_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCQ2284814.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(26029..27054)
                     /locus_tag="OHN27_000306"
     CDS             complement(26029..27054)
                     /locus_tag="OHN27_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2284815.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(27054..28148)
                     /locus_tag="OHN27_000307"
     CDS             complement(27054..28148)
                     /locus_tag="OHN27_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCQ2284816.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(28149..29963)
                     /locus_tag="OHN27_000308"
     CDS             complement(28149..29963)
                     /locus_tag="OHN27_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2284817.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            complement(30045..31601)
                     /locus_tag="OHN27_000309"
     CDS             complement(30045..31601)
                     /locus_tag="OHN27_000309"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCQ2284818.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGIEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(31782..32348)
                     /gene="mepS"
                     /locus_tag="OHN27_000310"
     CDS             complement(31782..32348)
                     /gene="mepS"
                     /locus_tag="OHN27_000310"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCQ2284819.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(32760..33473)
                     /gene="lpxT"
                     /locus_tag="OHN27_000311"
     CDS             complement(32760..33473)
                     /gene="lpxT"
                     /locus_tag="OHN27_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCQ2284820.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(33512..34498)
                     /gene="yeiR"
                     /locus_tag="OHN27_000312"
     CDS             complement(33512..34498)
                     /gene="yeiR"
                     /locus_tag="OHN27_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCQ2284821.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADSVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(34616..36082)
                     /locus_tag="OHN27_000313"
     CDS             complement(34616..36082)
                     /locus_tag="OHN27_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCQ2284822.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(36305..36877)
                     /gene="yeiP"
                     /locus_tag="OHN27_000314"
     CDS             complement(36305..36877)
                     /gene="yeiP"
                     /locus_tag="OHN27_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCQ2284823.1"
                     /translation="MPRANEIKKGMVLNYNSKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            37032..37286
                     /locus_tag="OHN27_000315"
     CDS             37032..37286
                     /locus_tag="OHN27_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCQ2284824.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(37283..38464)
                     /gene="setB"
                     /locus_tag="OHN27_000316"
     CDS             complement(37283..38464)
                     /gene="setB"
                     /locus_tag="OHN27_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCQ2284825.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNANFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            38832..39962
                     /gene="fruB"
                     /locus_tag="OHN27_000317"
     CDS             38832..39962
                     /gene="fruB"
                     /locus_tag="OHN27_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCQ2284826.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            39962..40900
                     /gene="fruK"
                     /locus_tag="OHN27_000318"
     CDS             39962..40900
                     /gene="fruK"
                     /locus_tag="OHN27_000318"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCQ2284827.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            40917..42608
                     /gene="fruA"
                     /locus_tag="OHN27_000319"
     CDS             40917..42608
                     /gene="fruA"
                     /locus_tag="OHN27_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCQ2284828.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            43032..43973
                     /locus_tag="OHN27_000320"
     CDS             43032..43973
                     /locus_tag="OHN27_000320"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCQ2284829.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            43961..44899
                     /gene="psuG"
                     /locus_tag="OHN27_000321"
     CDS             43961..44899
                     /gene="psuG"
                     /locus_tag="OHN27_000321"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCQ2284830.1"
                     /translation="MSKLKISPELLQISPEVQEALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            44993..46243
                     /locus_tag="OHN27_000322"
     CDS             44993..46243
                     /locus_tag="OHN27_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCQ2284831.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(46311..47009)
                     /gene="yeiL"
                     /locus_tag="OHN27_000323"
     CDS             complement(46311..47009)
                     /gene="yeiL"
                     /locus_tag="OHN27_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCQ2284832.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLTAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            47139..48080
                     /gene="rihB"
                     /locus_tag="OHN27_000324"
     CDS             47139..48080
                     /gene="rihB"
                     /locus_tag="OHN27_000324"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCQ2284833.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFESLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            48180..49430
                     /locus_tag="OHN27_000325"
     CDS             48180..49430
                     /locus_tag="OHN27_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCQ2284834.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLTGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(49486..50574)
                     /locus_tag="OHN27_000326"
     CDS             complement(49486..50574)
                     /locus_tag="OHN27_000326"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCQ2284835.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(50577..51434)
                     /gene="nfo"
                     /locus_tag="OHN27_000327"
     CDS             complement(50577..51434)
                     /gene="nfo"
                     /locus_tag="OHN27_000327"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCQ2284836.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(51508..52557)
                     /locus_tag="OHN27_000328"
     CDS             complement(51508..52557)
                     /locus_tag="OHN27_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCQ2284837.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            52656..53537
                     /locus_tag="OHN27_000329"
     CDS             52656..53537
                     /locus_tag="OHN27_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2284838.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            53742..55211
                     /gene="lysP"
                     /locus_tag="OHN27_000330"
     CDS             53742..55211
                     /gene="lysP"
                     /locus_tag="OHN27_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCQ2284839.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            55505..57496
                     /gene="cirA"
                     /locus_tag="OHN27_000331"
     CDS             55505..57496
                     /gene="cirA"
                     /locus_tag="OHN27_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCQ2284840.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFSDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(57528..58364)
                     /gene="yeiG"
                     /locus_tag="OHN27_000332"
     CDS             complement(57528..58364)
                     /gene="yeiG"
                     /locus_tag="OHN27_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCQ2284841.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            58622..59290
                     /gene="folE"
                     /locus_tag="OHN27_000333"
     CDS             58622..59290
                     /gene="folE"
                     /locus_tag="OHN27_000333"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCQ2284842.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            59307..60464
                     /locus_tag="OHN27_000334"
     CDS             59307..60464
                     /locus_tag="OHN27_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCQ2284843.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            60606..61646
                     /gene="galS"
                     /locus_tag="OHN27_000335"
     CDS             60606..61646
                     /gene="galS"
                     /locus_tag="OHN27_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCQ2284844.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            61926..62924
                     /gene="mglB"
                     /locus_tag="OHN27_000336"
     CDS             61926..62924
                     /gene="mglB"
                     /locus_tag="OHN27_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCQ2284845.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            62985..64505
                     /gene="mglA"
                     /locus_tag="OHN27_000337"
     CDS             62985..64505
                     /gene="mglA"
                     /locus_tag="OHN27_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCQ2284846.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            64521..65531
                     /gene="mglC"
                     /locus_tag="OHN27_000338"
     CDS             64521..65531
                     /gene="mglC"
                     /locus_tag="OHN27_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCQ2284847.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(65774..67009)
                     /gene="preA"
                     /locus_tag="OHN27_000339"
     CDS             complement(65774..67009)
                     /gene="preA"
                     /locus_tag="OHN27_000339"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCQ2284848.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPVLVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(67003..68241)
                     /gene="preT"
                     /locus_tag="OHN27_000340"
     CDS             complement(67003..68241)
                     /gene="preT"
                     /locus_tag="OHN27_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCQ2284849.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(68436..68675)
                     /locus_tag="OHN27_000341"
     CDS             complement(68436..68675)
                     /locus_tag="OHN27_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCQ2284850.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(68678..69397)
                     /gene="sanA"
                     /locus_tag="OHN27_000342"
     CDS             complement(68678..69397)
                     /gene="sanA"
                     /locus_tag="OHN27_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCQ2284851.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(69547..70431)
                     /gene="cdd"
                     /locus_tag="OHN27_000343"
     CDS             complement(69547..70431)
                     /gene="cdd"
                     /locus_tag="OHN27_000343"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCQ2284852.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(70561..71256)
                     /locus_tag="OHN27_000344"
     CDS             complement(70561..71256)
                     /locus_tag="OHN27_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCQ2284853.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(71253..71651)
                     /locus_tag="OHN27_000345"
     CDS             complement(71253..71651)
                     /locus_tag="OHN27_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCQ2284854.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            71890..72840
                     /gene="dusC"
                     /locus_tag="OHN27_000346"
     CDS             71890..72840
                     /gene="dusC"
                     /locus_tag="OHN27_000346"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCQ2284855.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTCLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            complement(73228..73311)
                     /gene="yohP"
                     /locus_tag="OHN27_000347"
     CDS             complement(73228..73311)
                     /gene="yohP"
                     /locus_tag="OHN27_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCQ2284856.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            73535..74971
                     /gene="mdtQ"
                     /locus_tag="OHN27_000348"
     CDS             73535..74971
                     /gene="mdtQ"
                     /locus_tag="OHN27_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCQ2284857.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            75024..75785
                     /locus_tag="OHN27_000349"
     CDS             75024..75785
                     /locus_tag="OHN27_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2284858.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(75915..76493)
                     /locus_tag="OHN27_000350"
     CDS             complement(75915..76493)
                     /locus_tag="OHN27_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2284859.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            76663..77250
                     /locus_tag="OHN27_000351"
     CDS             76663..77250
                     /locus_tag="OHN27_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCQ2284860.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCVLVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            77424..78356
                     /gene="pbpG"
                     /locus_tag="OHN27_000352"
     CDS             77424..78356
                     /gene="pbpG"
                     /locus_tag="OHN27_000352"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCQ2284861.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADSSRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(78395..80110)
                     /gene="dld"
                     /locus_tag="OHN27_000353"
     CDS             complement(78395..80110)
                     /gene="dld"
                     /locus_tag="OHN27_000353"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCQ2284862.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            80306..>80820
                     /locus_tag="OHN27_000354"
     CDS             80306..>80820
                     /locus_tag="OHN27_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="catalyzes the hydrolysis of terminal
                     beta-D-glucosyl residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HCQ2284863.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRAS"
BASE COUNT        19535 a        20002 c        21570 g        19713 t
ORIGIN      
        1 aaccatttga aatgtgagca aaagcccgtt tttgccacgc ttcccgccac tcttttgatc
       61 ctgctacagg ttttaccccg atcggggtat gcatctttga cacatccttt aatatcttag
      121 cggctataaa aatggcttat taattatgcg gtttatttgg ccgctctcaa ttttcagagc
      181 gcgttaatga tggaaggtca atgtgaagat tgatgcatcc cgtcggggca tactcactgg
      241 tcgctggcgc aaagccagta acggtatccg tccgccctgg tccggtgatg aatctcattt
      301 tctgacccat tgcacccgtt gcgacgcctg tattaatgct tgtgaaaaca acattctgca
      361 acgcggcgcg ggcggctatc cgagcgttaa tttcaaaaat aacgagtgca gcttctgtta
      421 cgcctgcgcg caggcttgcc ccgaatcgct attttctccg cgccacacca gggcctggga
      481 tttgcagttc accattgggg acgcgtgcct ggcgtatcag tcagttgaat gccgccgctg
      541 tcaggatagc tgtgagccaa tggcaattat ctttcgcccg acgctctccg ggatctacca
      601 gccgcaactt aatagccaac tttgcaacgg atgtggagca tgtgcagcca gctgtccggt
      661 atcagccata actgcggagt atctccatgc acactaactg gcaagtttgc agcctggtcg
      721 tgcaggccaa aagcgaacga atttcagaca tcagcaccca actgaacgcc tttcccggct
      781 gtgaagttgc tgtcagcgac gcgccgagcg gtcagttgat tgtggtggtg gaagcagaag
      841 acagcgaaac gctgatccaa accattgagt cagtacgcaa cgtagagggc gtgctggcgg
      901 tgtcgctggt ttatcaccag caggaagagc aaggtgagga aacaccatga aactcagtcg
      961 tcgtagcttt atgaaagcta acgccgttgc ggccgctgcg gcggctgccg gtctcagcgt
     1021 gccgggcgtt gcccgcgccg ttgttggtca gcaggaagcc attaaatggg ataaagcgcc
     1081 gtgccgtttc tgcggtactg gttgcggcgt tctggtcgga acgcagcagg ggcgtgtggt
     1141 ggcctgtcag ggcgacccgg acgcaccggt taaccgtggc ctgaactgca ttaagggcta
     1201 tttcctgccc aagatcatgt acggtaaaga ccgtttgacg cagccgctgc tgcgtatgaa
     1261 aaacggtaaa tatgacaaag aaggcgaatt taccccaatc acctgggatc aggccttcga
     1321 tgtgatggaa gagaaattca aaaccgccct gaaagaaaaa ggaccggaat cgatcggtat
     1381 gttcggttct ggtcagtgga ctatctggga aggttatgcc gcgtccaagc tgttcaaagc
     1441 gggcttccgt tcgaacaaca tcgacccgaa cgcacgtcac tgtatggcgt cggcagtagt
     1501 tggctttatg cgtacctttg gtatggatga gccgatgggc tgctatgacg acatcgagca
     1561 ggctgacgcg tttgtgctgt ggggcgctaa catggcggag atgcacccga tcctctggtc
     1621 acgcatcact aaccgtcgtc tctctaacca gaacgtcacc gtggcggtgc tttctaccta
     1681 ccagcatcgt agcttcgagc tggcggataa cggcatcatc tttacgccac agtctgacct
     1741 ggtgatcctg aactacatcg ccaactatat cattcaaaac aatgcgataa atcaggactt
     1801 cttcagcaag cacgttaacc tgcgcaaagg ggcgacggac atcggctacg gtttacgtcc
     1861 gacccatccg ctggaaaaag cagcgaagaa tccgggttct gacgcctccg aaccgatgag
     1921 ctttgaagat tacaaagcct tcgttgccga gtatacgctg gaaaaaactg ccgaaatgac
     1981 cggcgtgccg aaagaccagt tagaacaact ggcgcagctg tatgccgatc cgaacaagaa
     2041 agtcatctcc tactggacga tgggcttcaa ccagcatact cgtggcgtgt gggccaacaa
     2101 cctggtctac aacctgcacc tgctgaccgg caaaatttcc cagccgggtt gcggtccgtt
     2161 ctccctgacc gggcagcctt ccgcgtgtgg tactgcgcgt gaagtgggca cctttgctca
     2221 ccgtctgcct gcggacatgg tggtgactaa cgagaaacac cgtgatattt gcgagaagaa
     2281 gtggaatatc ccgagcggca ccattccggc gaaaatcggt ctgcacgcgg tggcacaaga
     2341 ccgtgcactg aaagacggca agctgaatgt ttactggacc atgtgtacca acaacatgca
     2401 ggccgggccg aacattaatg aagagcgtat gccgggctgg cgcgatccgc gcaacttcat
     2461 catcgtctcc gatccgtatc cgacagtcag tgcgctggca gccgacttga tcctgccgac
     2521 cgcaatgtgg gtagagaaag agggcgctta cggtaacgcc gaacgccgta ctcagttctg
     2581 gcgtcagcag gtacaggcgc caggcgaagc gaaatcggat ctctggcagt tagtccagtt
     2641 ctcccgccgc ttcaaaactg aagaagtatg gccggaagag ctgctggcga agaaaccgga
     2701 actgcgtggc aaaacgctgt acgaagttct gtatgccacc cccgaagtga gcaaataccc
     2761 tgtatccgaa ctggcggaag atcagctgaa cgatgaatcc cgcgagctgg gcttctatct
     2821 gcaaaaaggt ctgttcgaag agtacgcatg gtttggtcgc ggtcacggtc acgatctggc
     2881 accgttcgat gactaccaca aagcgcgcgg tctgcgctgg ccggtggtga acggtaaaga
     2941 aacgcagtgg cgttacagcg aaggtaacga cccgtacgtg aaagcgggcg aaggctacaa
     3001 gttctacggt aaaccggatg gcaaagcggt gatcttcgcg ctgccgttcg aaccggcggc
     3061 ggaagcaccg gatgaagagt acgacctgtg gctctctacc ggacgcgttc tggagcactg
     3121 gcacaccggc agtatgactc gccgtgtacc agaactgcac cgcgccttcc cggaagcggt
     3181 cctgtttatt cacccgctgg atgcgaaagc gcgcgatctg cgtcgtggcg acaaagtgaa
     3241 agtggtttct cgccgtggcg aagtgatctc gattgtcgaa acgcgcggtc gtaaccgtcc
     3301 gccgcagggc ctggtgtaca tgccgttctt cgacgccgca cagctggtta acaaactaac
     3361 gctggacgcg accgatccgc tctcgaaaga gacggacttc aagaagtgcg cagtcaaact
     3421 ggagaaggtg taagccatta tgtcccggtc agcgaaacct caaaatggtc gccgccgctt
     3481 tctgcgcgat gttgttcgca cagcaggcgg gctggctgcc gtgggtgtgg cgctggggtt
     3541 acaacagcaa accgcacgcg catctggcgt gcggttgcgc ccgcccggag ccataaacga
     3601 gaacgccttt gccagtgcct gtgtgcgttg cggtcagtgt gttcaggctt gcccttacga
     3661 caccttaaaa ctggcgacgc tggcctctgg tctgtcggcg ggcacgccat attttgtcgc
     3721 acgggatatt ccttgcgaaa tgtgtgagga cattccgtgc gccaaagtgt gcccaagcgg
     3781 tgcgctggat cgtgagattg aatcgatcga cgacgcgcgg atggggctgg cggtactggt
     3841 ggaccaggaa aactgtctca actttcaggg gctgcgctgc gatgtttgtt atcgcgaatg
     3901 cccgaaaatt gatgaggcca tcaccctgga gctggagcgc aacacgcgta ccggtaagca
     3961 cgcccgcttt ctgccgacgg ttcacagcga cgcctgtacc ggttgcggta agtgcgaaaa
     4021 agtatgcgtg ctggaacaac cggcaatcaa ggtgttaccg ctgtcactgg cgaaagggga
     4081 gttaggtcac cattaccgct tcggttggct ggaggggaac aatggcaaat cgtaaacgtg
     4141 acgccgggcg cgaggcgctg gagaaaaaag gctggtggcg cagtcaccgt tggctggtgt
     4201 tacgtcgcct ttgtcagttc ttcgtgctgg ggatgttttt gagcggtcca tggtttggtg
     4261 tgtggatctt gcacggcaac tacagcagta gcctgttatt cgacaccgtt ccgctgaccg
     4321 atccgctgat gacgctgcaa agtcttgcca gtggtcatct gcctgccacg gtggcgttga
     4381 ccggtgcggt gattatcacc gtgctctacg ccctggcggg aaagcgatta ttttgcagct
     4441 gggtctgccc gctgaatccg attaccgact tagcgaactg gttacgcagg cgctttgacc
     4501 tcaatcagtc tgcgacgatc cctcgccaca ttcgctacgt gctgttggtg gtcattctgc
     4561 tgggatcggc cttaactggc acgctcatct gggaatggat caaccccgta tctctgatgg
     4621 ggcgcagcct ggtaatgggc ttcggcagcg gcgcgctgct tattctcgcg ctgtttttat
     4681 ttgatctact ggtcgttgaa cacggctggt gcgggcacat ttgtccggtt ggcgcgctgt
     4741 atggcgtgct gggtagcaaa ggtgtgatta ccgttgcggc caccgatcgg cagaaatgta
     4801 accgctgtat ggattgtttt catgtttgcc cggaaccgca tgtgctacgt gccccggtgc
     4861 tggatgaaca aagcccggtg caggtcacca gccgcgattg catgacgtgc ggtcgctgcg
     4921 tggatgtctg ttctgaggat gtatttacaa taactacacg atggagttcg ggagcgaaat
     4981 catgaaaagc catgacctga agaaagcgct gtgtcaatgg acggcgatgc tggccctggt
     5041 ggtaagcggc gcggtttggg cggctaatgg cgtcgatttt agccagtcgc cggaagtatc
     5101 cgggacacag gaaggggcca ttcggatgcc aaaagagcag gaacggatgc cgctgaacta
     5161 tgtgaatcag ccgccaatga tcccgcatag cgttgaaggt tatcaggtaa cgaccaatac
     5221 caaccgctgc ttgcagtgcc acggtgtcga aagttatcgc accactggcg cgccgcgtat
     5281 cagtcctact cactttatgg acagcgacgg caaagtgggc gcggaagtgg ctccgcgtcg
     5341 ttatttctgt ctgcaatgtc acgtaccgca ggccgatacc gcgccaatcg tggggaatac
     5401 ctttacccca tcaaaaggtt acgggaaata agaggttatt atgggaaatt ctgaccgtaa
     5461 gcctggtctg attaagcgcc tgtggaaatg gtggcgtacc cccagccgtc tggcgctggg
     5521 gacgctgctg ttgatcggtt ttgttggcgg catcgtcttc tggggtggct ttaacaccgg
     5581 gatggaaaaa gccaataccg aagagttctg cattagctgc cacgaaatgc gcaacacggt
     5641 gtatcaggaa tacatggatt ccgtgcacta caacaaccgt agcggcgtcc gtgcgacctg
     5701 tccggattgt cacgttccgc acgagtttgt gccgaagatg atacgcaagc tcaaagcaag
     5761 taaagagctg tatggtaaaa tttttggcgt tattgacacg ccgcagaaat ttgaagctca
     5821 tcgtctgacg atggcacaga atgagtggcg gcgcatgaag gacaataact cgcaggagtg
     5881 ccgtaactgt cacaacttcg agtatatgga tacaaccgcc cagaaatcgg ttgccgcgaa
     5941 gatgcatgac caggcggtga aagatgggca aacctgtatt gattgccata aagggatagc
     6001 gcacaagctg cccgatatgc gtgaagtcga gccaggtttt taacagggtt attgcgtggg
     6061 tatgcttgaa gccagagagt tactttgtga gcgggatgaa cgaaccttat ttagtggctt
     6121 gtcatttacg ctgaacgcag gagagtgggt acaaatcacc ggtagcaacg gcgcggggaa
     6181 gacaacgctt ctccgtttgc tgactgggtt gtctcgccct gacgctggcg aggttctctg
     6241 gcaagggcag cccttgcatc aggtacgcga cagctaccat caaaacctgt tatggatagg
     6301 ccatcagccg gggatcaaaa cccggctgac ggcgttagaa aatctgcact tttatcatcg
     6361 cgatggcgat gccgcacaat gtctggaagc cctggcgcag gccgggcttg ccggattcga
     6421 agatattccg gtaaatcaac tttctgccgg gcaacaacgc cgcgtcgctt tagcgcgtct
     6481 gtggctgacc cgtgccacgt tatggatcct cgacgagcct tttaccgcga ttgacgttaa
     6541 cggcgtcgat cgtctgaccc agagtatggc gcagcatacg gagcaggggg ggattgtgat
     6601 tctgactacc caccagccgc tcaacgttgc tgaaagtaaa attcgccgca tttcactgac
     6661 gcaaacgggg gccgcatgat gttctggcgc attttccgtc ttgagctgcg cgtagcgttt
     6721 cgccatagcg ccgaaatcgc caacccgctg tggttcttcc tgattgtaat tacccttttt
     6781 ccgctcagta tcggtccgga gccgcaactg ctggcgcgta ttgcaccggg cattatctgg
     6841 gttgctgcgc tgctttcatc cttgctggcg ctggaacgac tgttccgtga cgatttgcag
     6901 gacggcagtc ttgaacaatt gatgttgttg ccgttaccct tgcccgccgt tgtgctggcg
     6961 aaggtgatgg cgcactggat ggtaaccggt ctgccgttac tcatcctttc gccactggta
     7021 gcaatgctac tgggaatgga tgtttatggc tggcaagtga tggcgctgac gctgctgctg
     7081 ggaacgccta cgcttggctt tctcggtgca ccgggtgtgg cgttgactgt gggacttaag
     7141 cgcggtggtg tgctgctcag catactggtg ttaccgctga ctatcccatt actcatcttt
     7201 gccaccgccg cgatggacgc ggcttccatg catttgcccg ttgacgggta tctggcaatt
     7261 ttaggcgcgt tgctggcagg caccgcgaca ttaagtcctt ttgcgacggc ggcagcgtta
     7321 cgaatcagca ttcaataacg ctggattact gatttttgtt cgagtctggt atcgaaacta
     7381 tgtggaaaac actgcatcaa ctggcgatcc caccacggct gtatcaaatc tgtggctggt
     7441 ttataccgtg gctggcaatt gccagtgtgg tcgtgcttac cgtcggctgg atctggggat
     7501 tcggctttgc tccggctgat tatcagcagg gaaatagcta ccgcattatc tacctgcatg
     7561 tgcctgcggc gatctggtcg atgggcattt atgcatcaat ggcagtggca gcgtttattg
     7621 gccttgtctg gcagatgaaa atggccaacc tggcggtggc ggcgatggcc cccattggtg
     7681 ccgtgtttac ctttattgcc ctggttaccg gctctgcatg gggaaaaccg atgtggggca
     7741 cctggtgggt atgggatgca cgtctgactt ctgaactggt gctgctgttt ttgtatgtgg
     7801 gtgtgattgc cctgtggcac gccttcgacg accgccgtct ggcgggccgt gcggcaggta
     7861 tcctggtgct gattggcgtg gtgaatctgc cgattattca ttactccgtg gagtggtgga
     7921 acaccctgca tcagggatca acgcggatgc agcaaagtat cgatccggcg atgcgttcgc
     7981 cgctgcgctg gtcgattttt ggcttcctgc tcctgtctgc cacgctgacg ctgatgcgga
     8041 tgcgtaattt gattttgctg atggaaaaac gccgtccgtg ggtgagtgaa ctgatactga
     8101 aaagaggccg taaatgaccc ctgcatttgc ttcctggaat gaatttttcg caatgggcgg
     8161 ttacgccttt tttgtctggc tggcggtggt gatgaccgtt attccgctgg tggttttggt
     8221 cgtgcactcg gtgatgcaac atcgcgcaat tctgcgtggt gtggcgcaac agcgggcgcg
     8281 tgaggcgcgt ttacgtgctg cgcaacagca ggaggctgca tgaatattcg ccgtaaaaac
     8341 cgcttgtgga ttgcctgtgc cgtgttggca gggctggcgc tgactatcgg tctggtgcta
     8401 tatgcgctgc gctcgaatat cgatctcttt tatacgccgg gggaaattct ctacggcaag
     8461 cgtgaaactc agcaaatgcc ggaagtcggt cagcgtctgc gcgttggcgg gatggtgatg
     8521 ccgggtagtg tgcagcgcga tcccaattcg ctgaaagtga ccttcactat ttacgatgct
     8581 gaaggctcag tggatgtctc ttacgaaggc attttgccgg atctgttccg tgaagggcag
     8641 ggcgttgtgg tgcagggcga gctggaaaaa ggcaatcata tcctcgcgaa agaagtgctg
     8701 gcgaaacacg acgaaaacta cacgccgcca gaagttgaga aagcgatgga agctaaccac
     8761 cgtcgcccgg cgagtgttta taaggaccca gcatcatgat gccggaaatt ggaaacgggc
     8821 tgctgtgcct ggcgttaggg attgcgctgc tgctgtccgt gtatccgcta tggggcgtgg
     8881 cgcgcggaga tgcgcgcatg atggcgtctt cccgcttgtt tgcctggctg ctgtttatgt
     8941 ctgtggctgg cgcatttctg gtgctggtca atgctttcgt ggtcaacgac tttaccgtca
     9001 cctatgttgc cagcaactcc aatacccagc ttccggtgtg gtatcgcgtg gcggctacct
     9061 ggggcgcgca tgaaggctcg ctactgctgt gggtgctgct gatgagcggc tggacctttg
     9121 cggtggcgat ttttagtcag cgtattccgc tggatattgt ggcccgcgta ctggcgataa
     9181 tggggatggt cagcgtcggc tttttgctgt tcattctctt tacctctaac ccattctcac
     9241 gcacgctgcc gaacttcccg attgaagggc gcgatcttaa cccgctgtta caggatccgg
     9301 ggctgatctt ccatccgcct ctgctttata tggggtacgt gggtttctcg gtggcgtttg
     9361 cttttgccat tgcttctttg ctgagcgggc gtctggacag cacttatgcg cgttttactc
     9421 gcccgtggac gctggcggcg tggattttcc tgacgctcgg cattgtgctc ggttccgcat
     9481 gggcctatta cgaactcggc tggggtggct ggtggttctg ggatccggta gaaaacgcct
     9541 cgtttatgcc gtggctggtg gggactgcgc tgatgcactc actggcggtc actgaacaac
     9601 gcgccagctt caaagcgtgg acattactgc tggcaatcag tgccttctcg ttgtgtctgt
     9661 tggggacttt cctcgtgcgt tccggcgtgc tggtatcggt acacgcgttt gcgtctgatc
     9721 cggcgcgcgg tatgtttatc ctcgccttta tggtgctggt gattggcggt tcgctgctgc
     9781 tgtttgccgc gcgtggacac aaagttcgct cacgcgtaaa caatgcgctg tggtcgcggg
     9841 aatctctgct gttagcgaac aacgttttgc tggtcgccgc gatgctggtg gtgttgctgg
     9901 ggacgctgct gccgctggtg cacaagcaac tgggactggg cagtatttcg attggcgaac
     9961 cgttcttcaa caccatgttt acctggctga tggtgccgtt tgcgctactg ctgggtgtcg
    10021 gtcctctggt gcgctggggg cgcgatcgcc cacgtaaaat ccgcaattta ttgattatcg
    10081 ccttcatctc tacgctggtg ctgtctctgc tgttgccgtg gctgttcgaa agcaaagttg
    10141 tggcgatgac ggtgctcggc ctggcaatgg cctgctggat tgcggtactg gcaattgcgg
    10201 aagctgcgct gcgtatttca cgcggcacga aaaccacctt cagttattgg gggatggtgg
    10261 cggcgcacct ggggctggca gtgacaattg ttggcattgc ttttagccag aactatagcg
    10321 ttgagcgtga tgtgcgcatg aagtccggcg atagcgtcga tattcatgaa tatcgcttca
    10381 ccttccgtga tgtcaaagag gtgactggcc cgaactggcg tggcggtgtg gcgactatcg
    10441 gcgtaacgcg agagggtaaa ccggaaacag tgctgtatgc ggaaaaacgt tattacaaca
    10501 ctgccgggtc gatgatgacc gaagcggcaa ttgacggcgg catcacgcgt gacctgtacg
    10561 ccgcgctcgg tgaagagctg gaaaacggcg cgtgggccgt gcgtctttac tacaaaccat
    10621 ttgttcgctg gatttgggcg ggcgggctga tgatggcgtt gggcggactg ctgtgtctgt
    10681 ttgatcctcg ctatcgtaag cgcgtgagtc cgcaaaaaac tgcgccggag gccgtatgaa
    10741 gcgcaaagta ttgttaattc cgttgattat cttcctggcg attgccgcgg cgctgctgtg
    10801 gcagctggcg cgtaatgccg aaggggatga tccgaccaat ctggaatcgg cgctcattgg
    10861 caagcctgtg ccgaagtttc gtctcgaatc actggacaat ccggggcagt tttatcaggc
    10921 ggatgtgctg actcagggca aaccagtact gcttaacgtc tgggcgacct ggtgtccgac
    10981 ctgccgtgcg gaacatcaat atctgaatca gctttcagcg cagggcattc gcgtggtcgg
    11041 catgaactat aaagacgatc gccagaaggc aatcagctgg ctgaaagagc tgggcaatcc
    11101 ttacgcgcta agcctgtttg atggcgacgg catgttaggg ctggatctcg gtgtctatgg
    11161 cgcgccagaa accttcctta ttgacggcaa cggtatcatt cgctatcgcc atgcgggcga
    11221 tctcaatcct cgcgtctggg aagaagagat caagccgctg tgggagaaat acagtaagga
    11281 ggccgcacaa tgaggttttt attgggcgtg ctgatgctga tgatctccgg ctcagcactg
    11341 gcgaccatcg acgtgttgca gtttaaagat gaagcacagg aacaacagtt ccgtcagctc
    11401 actgaagaac tgcgctgccc gaaatgccag aacaacagca ttgccgattc caactcgatg
    11461 attgccaccg acctgcgtca gaaagtgtat gaactgatgc aggaaggtaa aagtaaaaaa
    11521 gagattgtcg attatatggt ggcgcgttac ggcaacttcg tcacttacga tccgccgtta
    11581 acgccgctga ccgtgctgct gtgggtgctt ccggtagtgg ctattggcat tggcggttgg
    11641 gtcatatacg cccgctcgcg gcgtcgggta cgcgtagtgc cggaagcgtt tcctgaacaa
    11701 agcgtgcagg aaggtaagcg tgccggatat attgtttatc tgccgggtat tgtggtggcg
    11761 ttaattgtgg ctggcgtcag ctactacccg actggcaatt atcagcaggt gaaaatctgg
    11821 cagcaggcca cggcacaggc tccggcgttg ctggacaggg cgctggatcc gaaagccgat
    11881 ccgctcaacg aagaagagat gtcgcgtctt gcgctgggga tgcgtactca actgcaaaaa
    11941 aatccgggag atatagaagg ctggattatg ttgggccgcg ttggcatggc gctgggtaac
    12001 gctagtatcg ccaccgatgc atacgctact gcgtatcgcc tcgatccgaa aaacagcgat
    12061 gccgcactgg gttatgctga agcgttgaca cgttcatctg atcccaacga caaccgcctc
    12121 ggtggtgaac tgctgcgcca gttggtgaga agcgaccaca gcaatatccg tgtgttaagc
    12181 atgtatgcgt ttaatgcctt tgagcagcag cgatttggcg aagccgttgc cgcgtgggag
    12241 atgatgttga aactcttacc tgccaacgat actcgccgtg cggtgattga acgtagtatc
    12301 gcgcaggcga tgcaacattt gtcgccgcag gagagtaaat aaaaagaggc ccgatggtga
    12361 tgccatcggg ctatttttat tgtgccccgc gttgttgcag gaacagaatg gtggctgcca
    12421 cgcgtgagcg gacattgagt ttgcgcagca gattgcgaat atgtactttt actgtctgct
    12481 ctgaaatatt caacaccgag gcaatctgtt tatttgacag cccctgtgcc agctcgtgca
    12541 gaacatccag ctcgcgctcc gtcagcacgc tgaagggatc ttcttccgcg ccaaacattt
    12601 cacgttcacg taagtactga ttgacgcgtt cgctaaagac tttgctgcct ttcgctccag
    12661 cacgaattgc ttccagcaat acttccgggt cgctgtcttt caacagataa ccgtctgcgc
    12721 ctgcgtctat cagcgcaaag acatcgctgg aggcatctga tacggtcagg ataataattt
    12781 gcgcggtaac gccatccctg cgcaaggcat tgagggtatc caggccactc atacctttca
    12841 tattgagatc cagcaagatc acgtcgatat ccagtctatt cgccagatcg atcgcgctcg
    12901 cgccgtcgcc cgcttcggcg accacttcaa agccagaatc aagctccagt aactgacgaa
    12961 caccgcgtcg cataagtgga tgatcatcca caatcatcac ctgaaaaggc gttgcttcag
    13021 gcatagtagt ctcctgaggt tttattagaa tcattatgtt tgagcgtagc gttggtaacg
    13081 ctaccaggcg cgtgaatgtt accccatttt atgtatggac atgtagtgaa aactacaacc
    13141 ttgtcgttcg ctttggcatc attttcatta taaagacgga attttgcatc agcttctcat
    13201 caagaaaaac aactaaaaaa tcaatcacaa tacttgtgca tttctttaaa cagtttttaa
    13261 aaactgactc cgggtatgga gctatgggta ttttccgtac ccaatgcttt taacagcaat
    13321 taatttcata ggatgaaagc tcaatgcatc aatctggttc tgtttctctt tgtcgttccg
    13381 caatatctgt tctggtggct acagcgttat attcacccat agcattggca tcaacagttg
    13441 agtatggtga gacagttgat ggtgttgtcc tggaaaaaga tatccagctg gtttatggga
    13501 ccgccaataa tacgaaaatc aatcctggcg gagaacagca tataaaagaa tttggtgtaa
    13561 gtagtaatac tgaaattaaa ggagggtatc agtacattga aatgaatggt accgcagaat
    13621 actcagtatt aaatgacggt tatcaaattg ttcaaatggg tggcgcggca aaccagacta
    13681 ccctcaataa tggtgtgcta caggtttatg gcgcagcgaa tgatcccacg attaaaggcg
    13741 ggcgcttaat cgttgaaaaa gatgggatta ccgtccttgc cgctatcgaa aagggaggat
    13801 tactggaggt taaagagtgg ggattagcga ttgcggtaga tcagaaagca ggcggtgcta
    13861 ttaaagcaag cacgcgggtc atggaggtat tcggaacaaa ccgtctcggt cagttcgaaa
    13921 tcaagaatgg tattgttaac aatatgctgt tggaaaacgg cggaagtttg cgagttgaag
    13981 aaaatgactt cgcttataat actactgtag atagtggcgg cttactggag gttatggatg
    14041 gcgggactgc aactggcgtt gataaaaaag caggcggaaa attaattgtc tcaacgaatg
    14101 cgctggaagt gagtggtaca aacagtaaag gccaatttag tataaaagat ggtgtgtcaa
    14161 aaaattatga actggatgat ggttccgggc tcattgttat ggaggacacg caggccattg
    14221 atactatcct tgatgagcat gccactatgc aatcgctggg aaaggatact ggtacgaaag
    14281 tgcaggcaaa tgcggtatat gatctcggtc gatcagacca gaatggaagt atcacgtatt
    14341 cctctaaagc catctctgaa aatatggtta tcaacaatgg ccgcgctaat gtctgggctg
    14401 gcacaatggt taacgtgtca gtcagaggga atgatggcat tcttgaggtc atgaagccgc
    14461 aaataaatta tgcacccgct atgttggtgg gtaaggtagt ggtttctgag ggcgcttctt
    14521 ttagaacgca tggtgccgtg gataccagca aagcggacgt ttcgctcgaa aatagcgtat
    14581 ggaccatcat tgccgatatc actacgacga accaaaacac cctcctcaac ttagccaacc
    14641 ttgcgatgtc tgacgcaaat gtgattatga tggatgagcc agtgactcgt tcatcagtga
    14701 cggcaagtgc ggaaaatttc attacgttga ccaccaatac cctgtcggga aacggcaatt
    14761 tttatatgcg taccgatatg gctaatcatc agagcgatca gctcaacgtc accggtcagg
    14821 caacaggtga tttcaaaata ttcgtgacgg acaccggtgc cagcccggca gcaggagata
    14881 gccttacact ggtaacaacg ggcggcggtg atgctgcatt tacgttgggc aatgccggag
    14941 gcgttgttga tatcggtacg tatgaatata ccttgctgga aaatggcaac catagctgga
    15001 gtttggcaga gaatcgcgcg caaattaccc cttcaaccac tgatgtgctg aatatggcgg
    15061 ccgcacaacc gctggtattt gatgcagaac tggacaccgt gcgtgagcgt cttggtagcg
    15121 taaaaggcgt tagttacgat acggcgatgt ggagttcggc aattaacacc cgcaacaacg
    15181 tgaccactga tgcgggagct ggttttgagc aaacattgac gggcctgacg ctcggtatcg
    15241 atagccgttt ctcccgtgaa gaaagcagta caattcgcgg cttgatcttt ggttactctc
    15301 attctgatat tggttttgat cgcggcggca aaggtaatat cgatagctat accctggggg
    15361 attatgccgg ttgggagcat cagaacggtg cctatgttga tggggtggtg aaagttgacc
    15421 gttttgccaa caccatccat ggcaagatga gtaatggggc aacagcgttt ggcgattaca
    15481 atagtaacgg cgcgggtgct tatgttgaga gcgggttccg ttgggttgac ggattgtgga
    15541 gtgttagacc ctatctggcc tttaccggct ttaccacaga tggtcaggac tacacgttat
    15601 caaacggcat gcgcgcggat gtgggaaata cccggatatt acgcgctgaa gcgggaacgg
    15661 cggtaagcta tcacatggac ctgcaaaatg gtacgacgct ggaaccctgg ctgaaagccg
    15721 ccgtacgtca ggaatacgcc gattctaacc atgtgaaagt taatgacgat ggcaaattta
    15781 ataatgatgt ggctggaacc cgtggcgttt atcaggctgg gataaggtca tcgtttaccc
    15841 cgacgttaag cggtcatttg tcagtcagct atggcaatgg cgcaggggta gaatcgccgt
    15901 ggaatactca ggcgggtgtg gtctggacgt tctgataaca aaaaataaac aagctgtgat
    15961 gtgtcacggt ctgtttatcg aattaattgc agatataaaa aaaaccaacc gtaaaggttg
    16021 gttttttctt gggatttttg gtcggcacga gaggatttga acctccgacc cccgacaccc
    16081 catgacggtg cgctaccagg ctgcgctacg tgccgactcg tggctgctaa tactaccgtt
    16141 ttccacaccg attgcaagta agatattttg ctaactgatt tataattaat cagttagcga
    16201 taaaacgctt ctcgtctgtc agcacttgca ataacaaact taactgtggt ttttcatctt
    16261 tcactctttc accacgtaag ttgtaagtgc ggtatttacc gttattgttc agcaccagcg
    16321 tctttttcgg ggtggtaatt gccagcgtat cgttatccgc tgcagtaacc cagtaatgac
    16381 ggcgttgagg gttgaacaaa tcctgacctt gcgaatattc gctggcaggt gtgctgacat
    16441 gtagcaggcg ttgcatcagc gtcgtcatca gatcggtatg atcagtcaga gcattaatac
    16501 gctgcgccgg cgtgcctggc cagtgaatca ctaatggcac ctgcagatga ccgtgggacc
    16561 agtcaaaggt ttcttcctct tcgctcagtg gaataccccg accggcagtg ataatcacca
    16621 ccgtattgtc cagtttacca gaatcacgca gtgcattgag cacgcggttg atctggtcat
    16681 cgacattgcc tgccgcccgg ctatatttcc gtgcaaatgc ctgctgattg ctgtcgtcaa
    16741 tgttagtgcc attgaaagag acccacgaga accagcggtt atcttcttgt gcgtagcggc
    16801 ccagccagtt gatccactgc gtggcggtct gctcgtcgga ttgggtgcgt acgctcggca
    16861 tcgagaaatc tgacaacaat gcctggcgat acagcgggct ggtaaagcca tctgacgaga
    16921 ataaccccag ctgatagcct tgctgattaa gcgcagtaat taatgccgca ggcgtacggg
    16981 tcgacagaat gccgtccata tagctcggcg agatgccata gaacaggcca aagatgccgt
    17041 tgtctgtagt gttgccggag ctcatatggc gcgtgaacga aatattttgc tcagcaaaac
    17101 ctgccagcgc aggcatctgc ttctcgaagc gtgagtagtt caggccatcg acagtaatca
    17161 acagcacatt ctgcccggtg cccatatcgc gatagcgcag ttcgcttaac ggatactgta
    17221 cggaaacggc gtctggatta ccttgctcaa taagacggcg ttgatactcc tgcgcatcaa
    17281 gcaggccatg cttctcaagg aaacgtcgcg ccgtcatcgg gtacgaaagc ggcaggttag
    17341 cgcgctgcat ggtgatcggg cgatagaagt tggcatcggc ccagatatac accacatgcg
    17401 aggcgataaa ggcgataaat aagaatgcgg ccagcgggcg cgcgaagcgt cgacgacgcg
    17461 tcaggctgcg cagcttttgc cagctccacg tcgcaaacac cagttcaagc aataaaataa
    17521 ccggcacgct gatgaacatc agctgccagt cgcgcgccat ctcattttcg tctgggttga
    17581 taaccagttg ccagacgatg ggattaagat ggagatggaa acgagtaaag acttcgctat
    17641 cgatcagtaa tagcgtcatt cccgccgttg ccagaatgac ggacaaaaac ctcatcagcc
    17701 tctgggagcc gacgataaag gtcagcggga agaggatcag caagtaggtg gcgaacacca
    17761 ggaagctgaa atggccgata atgcttacgt aggaataaat gcgaccagca agcgttgtcg
    17821 gccagtcggc gataaacagg taacggctgc caatgacgag cgaaagcaga atattgaaca
    17881 gtgcaaacca gtgcccccaa ctgaccatct gggagacttt ttcacggtag cgctgacgat
    17941 gagttaccat aacctgttat ctgtttccct tagtgcgctt tgtcttcgtt gatagaggac
    18001 tgtaaggcgc gggcaaaaga gttggcaatt gcctggcgtt gggccggggc aatgctggtg
    18061 ttgataaggt tagtgaccat gttgcctaac accatcaggg aaaggtcggt cggagcctta
    18121 tgtttttcca gtacgttgag cagctctgcg agcaattgtt caacctgttc atcactgtag
    18181 cgggaaattt gtggcataaa tcgaaatcag ttagttgatg aaagggcaac atattaccgt
    18241 agcaacggtt ttttttctgc ttttttatgc gttaaatcat tctcgttacg ctgccttctg
    18301 taaaatgacc gccatctttt attgcgtcgc ttaaccggcg gtggttgaat accgcccggt
    18361 cttaaaggag agtttatcat gagtctggat atcaaccaga ttgccctgca ccagcttatc
    18421 aagcgtgatg agcagaacct tgagctggtg ttgcgcgatt cattgctgga accgacagaa
    18481 accgtcgttg agatggtggc agaactgcat cgggtctata gcgccaaaaa taaagcttac
    18541 ggcttgttta gcgaagagag tgaactggcg cagacgctgc gcttacagcg tcagggcgaa
    18601 gaggactttc tggcatttag ccgcgcggca accgggcgcc tgcgtgacga attagcgaaa
    18661 tatccttttg ctgacggtgg attcgtgctg ttttgccact accgttatct ggcggtggag
    18721 tatctgctgg tggcagttct gagcaacctg agcagtatgc gcgttaacga aaatctcgac
    18781 atcaacccaa cccattatct tgatatcaat catgcggata ttgttgcgcg tatcgattta
    18841 accgaatggg aaaccaatcc agagtccacc cgctatctca ctttcctgaa agggcgagta
    18901 gggcgcaaag tggcggattt ctttatggat ttcctcgggg ccagcgaagg attgaacgcg
    18961 aaagcgcaaa accgtgggct gttgcaagcg gttgatgatt tcactgccga agcacaactg
    19021 gataaagcag agcgacagaa cgtgcgccag caggtttata gctactgtaa tgagcagctg
    19081 caggcaggcg aagagatcga actggaatcg ctgtcgaaag agctggcagg cgtaagcgaa
    19141 gtgagcttca cggagtttgc ggctgaaaaa ggctacgaac tggaagagag cttcccggca
    19201 gaccgtagca cactgcgtca gttgacgaag tttgccggta gcggcggtgg cttgactatt
    19261 aactttgatg cgatgctgtt aggtgaacgc atcttctggg atccagcaac cgataccttg
    19321 accattaaag gcacgccgcc gaatttgcgc gaccaattgc agcgccggac gtctggcggc
    19381 aattaatcgg ttttgtgttc tgaatgccag atgcggcgtg aacgccttat ctggcctgca
    19441 aaaaatagca ataccgtaat caggcataat ctgcagatac aaaaaacccc gccggagcgg
    19501 ggttttttac aacttattca gcaattaagc gcgaacgaag tcgatgtgct gcagcttcgg
    19561 tttgtacggg tgacgctgta cgtcctgagc tttaactttg atttctttac cgtcaacaac
    19621 gatggtcaga acttcgctgt agaattcagc tttagcttgc atgttcatga ctttgtcgtg
    19681 atccagctcg atagccagcg gcgcttcttt gccaccgtag atgattgccg ggaacttgtt
    19741 agcggcacgc aggcggcggc tcgcaccctt accctgctct ttacgtactt ctgcgttgat
    19801 agtaaacatt tctttctctc ttaaattaat cctgctacag gcgacccagc aacaggtaag
    19861 tgatctgctt tgcgtatgca aaagcgggcg agattctata ctcaatcgcc ttatacatca
    19921 atcaaaacta ccattaaccg cgtaattcgt gcgcccgacg aaaacgacct tcgtaatcga
    19981 acactttttc acgcacttgc cagtactgac ctttcatgcg ggcgacgaca aaatccggat
    20041 gtcgcagtaa ggcttgctgg gcgaggatat cggcggcggt gatccagcgc agagggatgc
    20101 ccggtgtgcg cgtatgcggg cggataaaaa gctgctcgaa ggcggtacgc tgggcgggcg
    20161 tttgcagacg gaaacgctca ctcacatccg cgccgtcttc atcgtaatag gtgattttca
    20221 accattcgcc tttctcgtca tgcccatgct gtaaagacat gccgctacag cgtaatacca
    20281 gcgcgtcttt cagtcgtagc gccgctttta acatatcgtc tggatcaacc agtacggtgt
    20341 cacattcgcg gcagcggcga gcggcaatat cattttctgc gttacattgc gggcaatttt
    20401 taaaacggaa acggaagtca cactgttcgc gatgaccgtc gtcatcttca aaccatccct
    20461 gacagcgacg accaaagtgt tcaatcaatg tcccgtcggc ggtcgttttt ccccaaaagg
    20521 tgttggcaaa accgcagaca gggcagaaaa cctgtaccgg gacgttgtca cttttgcctt
    20581 tcggtgtacc aacttccggc gcgtagagat cgtgaggatt acccgcataa tcaagaatta
    20641 aacaatcagt cttgcccgga gcgagacgca gaccgcgccc gacaatttgt tggtaaagac
    20701 tcactgattc ggtaggacgc agaatggcga taagatcgac gtgcggggcg tcaaatccgg
    20761 tggtcagtac tgcgacgttg accagatagc gaaaacgctg ggctttaaaa tcttcaatta
    20821 acacatcgcg ctcagcgccg ggggtgtcgc cagtaatcag tgcagcatct tcggcaggca
    20881 gtaatcccac aatctctttt gcgtgttcaa cggtggcggc gaaaatcatt acccctttgc
    20941 gcttttcggc gaactccatg atctggctga tgatatgtgg cgtaatgcgt tgctgttttt
    21001 tcagctcacg gttgagatcg gcttcgctga acagcccgtt gctctgtgcc tgcaagcggc
    21061 tgaaatcgta ttgcactact ggcatatcca gtcgttctgg cggcgtcaga tagccgtgtt
    21121 taatcatata acgcagcggc agctcataaa tgcagtcacg gaaaagggct ttctcatcgc
    21181 cgcgtaccat gccgtgataa tggaactggt agatccagcc tttgcccaat cgaaaaggcg
    21241 tggcagtcag ccccagcagg cgtaagtggg gattcacttt tgtcaggtga gtgaggattt
    21301 gctgatactg gctctcttca tcgtcaccaa tacggtgaca ttcatcgaca atcaacagcg
    21361 aaaattcacc ctgaaaggca tcaagattac gggcgaccga ctgaacgctg ccaaatacca
    21421 ctttaccgtg gctctctttg cgctttagcc cggcggcaaa aatatcggct tccagcccca
    21481 gcgcctgata ctttgcatgg ttttgcgcca ccagttcttt aacgtgtgcc agcaccagta
    21541 cgcgaccacg agccagccgt gccagttccg ctatcaccag gcttttacct gcgccggtgg
    21601 gcagcacgat aacggcaggg gttttatgac gacgaaaatg gttgagcgtg gcatccacgg
    21661 cttcttgctg atatgggcga agtgtaaaaa tcatggtctc gcgtaggtag attattctgc
    21721 taatagtatg ccacggatct ttttcccttg agggatgtta ttagaccgct atactgggca
    21781 gacaacagtt cagattttcg ggtcattacc cgacctccgt tttttacagg caaaatcaca
    21841 cacatgcgac ttgataaatt tatcgcacag caactcggcg ttagccgtgc tattgccggg
    21901 cgtgaaatcc gcggcaatcg tgtcaccgtt gatggcgaaa tcgtccgcaa tgcagcgttc
    21961 aaactgctcc ctgaacacga tgttgcttat gatggcaatc cgctggcgca gcaacacggc
    22021 ccacgttatt tcatgctcaa caagccccag ggctatgttt gctcaactga tgacccggat
    22081 cacccaacgg tgctctattt tctggatgaa cctgtggcgt ggaaactgca cgcggcgggg
    22141 cggttggata ttgataccac cggtctggtg ctgatgactg acgacggtca gtggtcgcat
    22201 cgcattactt ctccgcgtca tcattgcgag aagacctatc tggtgacgct ggaatcaccc
    22261 gtagctgacg atacggcaga gcaatttgcc aaaggcgtac agctgcataa cgaaaaagat
    22321 ctcactaagc cagcggtgct ggaagtgatt acccctacgc aggtccgtct gaccatcagc
    22381 gaagggcgtt atcatcaggt gaaacgcatg ttcgccgctg tgggtaacca tgtggttgag
    22441 ctgcatcgtg aacgtattgg cggtattacg ctggatgctg atttagcccc cggtgaatat
    22501 cgtccgttaa ctgaagaaga aattgccagc gtcgtctaac ttttccatga ctttcaggag
    22561 cccgttgtga ccacccgaca gcattcgtcg tttgctattg tttttatcct tggcctgctg
    22621 gccatgttga tgccgctgtc gattgatatg tatctgcccg cgctaccggt aatttcagcg
    22681 cagtttggcg tacctgcggg cagtacgcag atgaccctca gtacttatat tctgggcttt
    22741 gcgttggggc agttaatcta cgggccgatg gcagacagct ttgggcgtaa gccggtggtt
    22801 cttggcggta cgctggtgtt tgccgccgcc gcggtggcgt gtgcgttggc acaaaccatc
    22861 gatcagctga ttgtgatgcg tttcttccac gggctggcgg cggctgcggc cagcgtggtt
    22921 atcaacgctt tgatgcgtga tatctacccg aaagaagagt tctcgcggat gatgtcgttt
    22981 gtcatgctgg tgacgaccat tgcaccgctg atggcaccga tagttggcgg ctgggtgctg
    23041 gtgtggctga gctggcatta catcttctgg atcctcgccg tggcggcgat tctggcttcg
    23101 gcaatgatct tctttctgat caaagaaacc ttaccaccgg agcgtcgcca gccttttcac
    23161 attcgtacca ctattggtaa ctttgcggcg ctgttccgtc ataagcgtgt cctgagttat
    23221 atgcttgcca gtggtttcag ctttgccggg atgttctcgt tcttaagcgc cggaccgttt
    23281 gtctacattg aaattaacca cgtcgcgccg gaaaactttg gttattactt tgcactaaac
    23341 attgttttcc tgttcgtgat gaccattttt aacagccgct tcgtccgccg tattggcgcg
    23401 ttaaatatgt tccgctcggg gttgtggata cagtttatta tggcggcgtg gatggtcatc
    23461 agtgcgctgc tggggctggg attctggtcg ctggtggttg gcgttgcggc gtttgtgggc
    23521 tgcgtgtcga tggtgtcatc caatgcgatg gcggtcattc ttgatgagtt tccccatatg
    23581 gcgggaacgg catcttcgct ggcaggaacc ttccgttttg gcataggggc aattgttggc
    23641 gcattgcttt ctcttgcgac ctttaactct gcatggccga tgatttggtc aattgcattc
    23701 tgcgcaagca gctccattct cttctgtctg tacgccagtc gaccgaaaaa acggtgatct
    23761 attgcacaac gaggaagcta aaaggcttcc tttgttgatg catgtcaacc acaaatctat
    23821 cattcccccg atatatgttt attttttgta aaatcaattt atgtaaaaag tcacatcatt
    23881 gtagttaaaa aggttgagtt agatcgcaga aacgggtaca tatagccccg caaacgtgac
    23941 cacgcccgca gatattactt aaatcagagc catagaggcc acgcaggcga ggcatcaatc
    24001 tttacgatct gtataaagac ggattgttga tgatgtgtta aaattgatgt aaacaaattg
    24061 tgaagtgaat gtgcttccgg ggaaaataag tgacttcatt acaactctca atcgtccatc
    24121 gactgccgca gaactatcgc tggtctgctg gtttcgcagg ttcgaaggtt gaaccgattc
    24181 cgcaaaatgg accgtgcggt gacaacagcc tggtggcgct taaattgctt agcccggatg
    24241 gtgataatgc atggtcggtg atgtataaac taagccaggc gttaagcgac atcgaagttc
    24301 catgttcggt gctggagtgt gaaggtgagc cgtgcctgtt tgtaaatcgc caggacgagt
    24361 ttgctgcaac atgccgattg aaaaattttg gtgtggcaat tgccgaaccc ttttcaaact
    24421 acaatccttt ttaagcgtca gctcaacgcc agtagctgac gcgtatactc ctgctgcggt
    24481 gtggcaaaca cgcgcgcgca cggtccttgt tccactactt ccccttgtcg cagtacgata
    24541 acctgatgac ataacgcgcg gacaacgtgc aaatcgtggc tgataaacaa ataggccagt
    24601 tgatgctttt gttgcaatga tttcaatagc gtcaatattt gcgcctgtac cgttttgtcg
    24661 agtgatgatg tcggttcatc aagtatgatc agcgagggct taagaattaa tgccctggca
    24721 atcgcaatac gttgtcgctg accaccagag aactccgccg gataacggtg gcgtgtttca
    24781 ggatctaatc ccacttcatg catcacggct atcacttgtt gttcgcgttg tgcggcagaa
    24841 agcgtcggct ggtgaacccg taagccttcc tcaataatct gcaaaacgtt gagtcgtggg
    24901 ttgagcgagg agtttggatc ctgaaatacc acctgaatgc gatgacgaat aggtaacagc
    24961 tggcggcgat ttaaattttg cagtggctga ccgtcaaaga tgatgctgcc ctgagaatta
    25021 atcagtcgca gcagcgccag tcccgtcgta cttttcccgg aaccggactc gcccactaaa
    25081 cccagtgttt cacccgctcg tagcgtaaaa ctgatgtttt tcaccaccac attatgatcc
    25141 acaatgcgct tcaaaatccc tttgcgaatg gggaaggcaa cctgaagctg ttcaacatcc
    25201 agcaacgttg aggcaggttc tggcaacggc actggatcgc ctgacggttc actgttgagt
    25261 agcttttgtg tgtaaggatg agtgggtgat gcaaatagcg tagcggcgta attttgctcg
    25321 acacagcgac cgttttgcat taccgccacg cggtgggcca gttttctgac aatgctgagg
    25381 ttatgagtaa taaacagcat gcccatattc agctcgcctt gcagttcgcg caacagctgt
    25441 aaaatctgcg cctggacaga gacgtccagt gcggtggtcg gttcatcggc aattaataat
    25501 tccggtcgcg ttaacagcgc catcgcaatc atcacccgct ggcgttcgcc gccggagagc
    25561 tgatgcggat aatctgtcag ccgttttgcc gcctggcgga taccaacgcg atcaaggcag
    25621 ttaagaattt cgccacgagc cgcttcccga cgcatcccgc ggtggagtga aagcacttca
    25681 taaagctgtt tttccagggt atgcaatgga tttaacgaca ccatcggttc ctgaaaaatc
    25741 atggcgatct tattaccgcg tacaccgcgc aacgtttgat cgctggcatg aagcagcgat
    25801 tcgccatgaa aacgaatatc gccggagaga tattcaaccg gcggggaagg gagcaggcgt
    25861 aaaattgaca gcgcggtaac gcttttgcct gaacctgact cacccaccag cgccagcgtt
    25921 tcgccagcct caatctgtag tgaaacatca ttgactactg tacgtacggt ttgctgatgg
    25981 cgaaaaccca ccgacaaatt ttcaatcgct aacagagttt gcgtcatgct acaccgcctt
    26041 attaggatca aatgcgtcgc ggacggcttc accaataaag atcagcaaag acaataatat
    26101 cgccaccgac aagaaggcgg tgatcccaag ccacggggcc tgaaggttat ttttcccttg
    26161 taacagcagt tcaccgagtg acggtgaacc gagcggtaga ccgaagccga ggaaatcgag
    26221 cgaggtcagg gtggttatcg aactacataa aataaacggt aaaaaggtga gggtcgcgac
    26281 catggcatta ggcaacatat gacgcaggat gatactgcga tcgctgacgc caagcgcctg
    26341 tgccgcacga atgtagtcga aattacgagt acgtaaaaac tccgcccgca ccacgccgac
    26401 cagactcatc cagccaaaca agacagtaat tgccagcagc caccagaagt taggctgtac
    26461 gacgctggaa agtaaaataa tcaaaaacag cgtcggcatc cccgaccata cttcaataaa
    26521 gcgttgtccc cagagatcga ctttaccgcc gtaatagcct tgtagcgccc ccgccagcac
    26581 gcccatcacg ctggaacaga gagtcagcat caggccaaac agaaccgaga tccgcgtgcc
    26641 atagagaatg cgtgccagca catcgccgcc gttggcatcc gttcccagcc agttttgccg
    26701 ggagggtgga gaagggaagg gcttatcggt agcaaagttg atactggtag caccaaagcg
    26761 aatcggtgcc cacagtaccc agccgttatt ttccagccgt tgtttcagcc acgggtcctg
    26821 ataatcagcc tgacttgcca gcgggccgcc aaaatcgctt tcgctgtagt ttttcaataa
    26881 cgggaaatac caactgccgt cataacgcac cagcaacggt ttatcgttgg cgataagttc
    26941 agaacacaaa ctcaaaccaa acaagacgag gaaaatccat aacgaccagt agccgcgacg
    27001 gttatgacga aaacgcgccc aacgggcctg attgacgggg ctgagtcgcg acattaacgt
    27061 ccctcaaaat caatacgcgg atcaaccagc gtatagctga tatcactgac aatattcagc
    27121 agcaggccaa tcagggtgaa aatatacaag gtaccaaaca ttacaggata atcgcgcgag
    27181 acggtcgctt cgtagcccag taagcccaga ccattgagtg aaaacatcac ctcaatcagc
    27241 agcgagccgg taaaaaacat gctgataaac gtcgccggaa aaccggcaat caccagcagc
    27301 atggcgttgc ggaacacatg tttccagaga atattttttt cacttacccc tttcgcacgt
    27361 gcggtcacca cgtattgctt gcgcacttca tcaaggaatg agttttttgt cagcatggtc
    27421 agcgccgcaa agccaccaat cactgtcgcc agcaccggca gcgtgatatg ccacagataa
    27481 tcggtgattt tctgatacca cggcagcgaa tcaaagttag cggaaaccag gccgcgtaga
    27541 gggaacaggt cgaaatagct gccgcccgcg aagaagacaa tcagcaggat ggcaaacaaa
    27601 aaggccggaa tggcgtagcc gatgatgata aatgcgctac tccagacgtc aaagcggctc
    27661 ccattataaa cagctttgcg aatgcctaac ggaatcgaca ccagatagat aatcagcgtg
    27721 ctccacaatc cgagggtgat ggaaaccggc agactgtctt taatcagcgt cagcaccgag
    27781 gcgctgcgaa acaggctatc gccaaaatca aagcggatgt agtcccagag cattttgaag
    27841 taacgttcgt ggatcggctt atcaaaaccg tagcgatgag tgatctcagc gatcacttct
    27901 ggatctaatc cgcgtccgcc acggtaatta ctgtcgctga tattgccgac acccgtttgc
    27961 gcatggctgg cacgaacacc ttcaccgcct gcgccgggta atactccggc attaccaaac
    28021 tcaatggcgg cgatggcctg gtcgaccgga ccgccaggcg caatttgcac gatgaaaaag
    28081 ttgatggtga taatcgccca taatgttggg atcaccagca acagacggcg aatcaggtaa
    28141 gcgcccatct actctccctg tttgctggcg gacggcagtt tggccgcttt attgacgtca
    28201 taccaccagg tatcgatacc gaggctatag atggggcgca cggccggctg ggagaattta
    28261 tcccaccagg cgagacggtc ttccgccatg taccacattg gcagcatgta ataattccac
    28321 gttaatacgc gatccagtgc tcgccccaac ggcagtaatt tttctttatt tccctgcgcg
    28381 gcaataattt ggttgatcag cgagtcgata accgggcttt gcacgccggg ggcattataa
    28441 gtggaattga tatattccga tgaccaggaa atctgtaaat cggaactggg ccacggcatc
    28501 gcccgccata cgcgcggcat catgtcatag tcgcgactgc gcatgcggtt agtgatttgc
    28561 gagttatcca ccttgcgaat gtccatgttg atacccagcc gttgcaggct gtgctggaac
    28621 ggcaataccc actgactatt gctgcttgcg ggaagcaata attcaaagct gagtggctga
    28681 cccgttgtgg cattaacgcg ttgctgaccc ttcagcaccc agcccgcttc gttgagaagt
    28741 ttgtcggctt ttaacaggtt gtcacgatcg tagccatcgc ctttggatac cggcggctgg
    28801 tagatttgtg tgaagacttc tggcggtaga tcttttttca ttggtgccag aagcaccagc
    28861 tccgcggcgt cggggtaatt tctggccgcg tattcggtat tctgaaaata actgttcgtg
    28921 cgactccagg cattgtaaaa caacgccttg ttcatccatt caaagtcaaa ggcgagagtg
    28981 attgcttccc gcacccggcg atcgctgaat actggacgtt ggatattaaa cgccagccag
    29041 cgcgtatcct gggctgattc gttcttttgc tcatctttga tgatgtattt tttatcgaaa
    29101 tttttaccgg tataacgcgt ggcccagttt ttggcgtcgt tttccatacg caaatcaaag
    29161 gcacctgctt taaacgcttc aaaggcgaca ttatcatcga ggtaataatc gtagcgaatg
    29221 gtgtcgaaat tccagcgtcc gcggtttacc ggtaagtttg ctgcccagta atctttcacg
    29281 cgggaataga caatattttg ccccattttc caggacgtaa tgcggtacgg accactggca
    29341 agcggaggcg tggcgagcgg gtcgctaagt ttgtgatcct tccagtactt ttctggaaat
    29401 accggcagcg aaaacagact caacatatct tctttgccgg gtttagctaa ctcaatgcgc
    29461 acggttaacg gtgcaatggc tttgacggtg gtgcctttgt agaccagacg aaattgcggc
    29521 acgccttcgg tcataaattt ttgaaaagta aactctacat cgcgggcagt aatgggcgaa
    29581 ccatcatgaa aacgggcgcg cggattaata gcgacctcca cccaggaata atcgtcagca
    29641 tagcgtgcgc tttcagcaat cagcgggtaa taactgcctg gttcgtcatc ggaagtcgta
    29701 aatagcgtgt cgtacagctg ctcggtgcgt gcgcccgggt tgccgcgcag tgcatagcgg
    29761 ttgaaattat cgaaggtgcc gagggctgac aacgttatct gcccaccttt tggcgcggcg
    29821 gggttcacat aatcaaaatg gttgaaatta aacgcgtacc ggggttcgcc cagcacggca
    29881 aaggcatagc tttccttgat agcctgcgcc tgcacaccaa aggtgaacag agcgataaac
    29941 agcagcagta tgcgcacaat catctgcaac ggatttcctt ttactcatca ttagccgcaa
    30001 tccggcatgc tttcactatg aataataagg gacgtaagca caccttacca ctgcggcgta
    30061 tacggtttct tcagccagcg gacaaaatcg tccagcggta aagggcgact aatccagtag
    30121 ccttgcatga aattaacgcc gcgttcgctt agccatcgcg cctgttccgg cgtttcgacc
    30181 ccttcagcaa ccgtcagcat attgaggcgt ttcgccagcg tcagcaccgc gtcaagtacg
    30241 ggtgaagtga tcgtttccgt accgatggcg ttgataaatc cacggtcaat tttcagataa
    30301 tcgagcgtaa aacgctcaag atagataagc gcgctgtgcc cggtgccgaa gtcatcaata
    30361 gcaatttcta tgccgaccga gtgcagccag gcgaagagtt gtgtggcttc ttgctctttc
    30421 agcatatcgc gctcggtaat ttccagcaca atctggaaat ggtgtgcggg cagggaagtg
    30481 agcagtttct ggatatctgc tttaaagctt tcgctgtgca gatggtccgg cgcaatgtta
    30541 ataccaaatt tgacgcctac tggcagcact ttttctaatt ctgcggcatc gcgggcaatt
    30601 aactcaaaca ggtgctgagt cagcggcaca atcatctttt gcgattcggc aaagttaatg
    30661 aaggcatccg ggggaatttc tcccgcgaca ggatgccgcc agcgtagcag tacttccagg
    30721 cccgttactc gcaaagcttg tgtatccacc accggttgat acgccacgta aaattgttcg
    30781 cgcttgatgg cggtcatgat ttctctgccg gggcgcatac gtacgctcat caggtaatag
    30841 cagagcaggc caacgacagt tccactcatg ccgcccagta aaaatgcgta ccacacatcg
    30901 ttccatgtcc agtcatccgc ataaagccga acggtcagag gaatgcccgc aattttagtt
    30961 tcacggaccg gcatgtcggt taattcatta acgttcatca aacgtgaaga aaaggtagat
    31021 agcgcagtat tgccaataat gagggcgacg ccatcgtaat cttcctgccg tgaactataa
    31081 aagagagaag gcgtcaggtt gagattcaga gcggcaaaca caccgctatt tttcagcaaa
    31141 gggttgcgat accagatgac gattgcgggt ttgttcggca ccatcggcgt gccgggtaag
    31201 atcgccatat cgacgttttt attaatgtcg agcgccggaa tcaattcatt gagtggaatg
    31261 tccatctcac cagtcgcaga tgaacagaat gtttttttat ctttcaccag cacaaacgta
    31321 cggacattca tgctaaacgc cgcgcgagcg gtcagttcgg ggttagcctg ctggcaggta
    31381 tctaaggtca gcggctgaag ccggtcagta gtggatttca ggtcggcaaa ataggtatcg
    31441 agatactttt gtacgtcgat aatcagtgtg tcgtatttga cttcgtgctt atgccaggcc
    31501 actaaaaact gaaggcagct aaccagtatc gcaatcatca cgcctgctac aatgcaggta
    31561 agcaggagct tacgtccaga attgggagcg cgtatgaaca tgattccgtt aatttcctga
    31621 aattacgaca acatttaaaa gataacaatg accggactat agcctggatc aagacgcgga
    31681 aacatcattt tctctgatga atgaaaacaa aaaagcactg cctaagcagt gctttcagtt
    31741 aactcgtcag gatagccaag ggattgcatc caaacggttt attagctgcg gctgagaacc
    31801 cggcgtgctt cgttgtaacg cttcttccag tacggttcat tcatgctgga aataataaca
    31861 ccactgctgg tggaagcatg gacaaactga ttgttgccga tataaatacc gacatggcgt
    31921 cccgttgaac cggcacggaa cagaactaaa tcacccgtac gcaaattact gcgggaaaca
    31981 gatttaccca tttcctgctg ttcgtaagtc gaacgcggaa gttctaagcc aaattgctca
    32041 cggaatgtac gctgtacgaa accagaacaa tcgatacctt ttttagtgct gccgcccaga
    32101 cgataacgta cgcctttcca gtcagcatac tgatccataa ttcgcgattt tacgtcgaca
    32161 ttacgaacca ggttttcaaa ttcatcctga gaagcctgca gtgatgatgt ttcactaccc
    32221 actgcacgtg tctcaggatg catattcttt gcggtgttat ttgcactaca tgcagaaagc
    32281 agaaccgcta ctgcaatcgc gggaatcccg cgcaagatat atctcaaaat cggttgagat
    32341 ttgaccatgt tgtttgtttt cccttgaagt ccttaacgac aaatatcgtt ataaaaaaat
    32401 gccaagcgac acgagactaa ttacctgggc atgttgaaac aattccttaa ggccaaatct
    32461 gtggactaac gcacaaaatt atatgctgtg aggataatct accgccgagg caaaacgagt
    32521 tcgagattac ccgattggtg cggtcttggc gagcggtttt acatgatttt ttataagaaa
    32581 aagtgataca gaaagttaat aagcgggatt ggtcaattaa agtacgattg agtgaaacgc
    32641 atgcaatcca ttcatttact tagagaatga tttttgcgtt cgatagatga aagcacggtg
    32701 cgcatcatca ggattatcct cactataaaa ataaacctga tgatgttaat tactgtgagt
    32761 tatttgtttt ggaaatgttt gttttttccc ggtagtgatt tgtcaaaaaa ggtgattaat
    32821 cgatcactta atggcgtcag caacacccag ggcaaaccga tcaatatcac ggtcatcgaa
    32881 ccgacaatga tgtcagtaaa ccagtgtgcg ccaatcatta ctctgggaaa cgcaaaaacc
    32941 acaaaaataa taagggcgat aagacctgca actttgccga aataacgcca catgaatgcc
    33001 gaaaaaataa gcagcatcat gccgtgatcg ccgggaaagc tatctcgtga ggcatctttc
    33061 gtggggacag agagcagttc gctgacgcgg ttaatattag taaacgtcaa tgttgggctg
    33121 gcccgtttta caggaattaa tgcctgaccc agctggttta ataccactgc agttaatagc
    33181 atgaccagac caataatcac gatacgtcgt ctgcctgggg cgttttcttt cagccagaaa
    33241 ctcagcatca acattcccat cgccagcaga gaacaaccgt cgaaagcgcg attgttggtc
    33301 aatgcaacca gccataaaaa ggccttactt tcgaccagtt tctgattaaa gaaataaaaa
    33361 atatccgcat caatcggcaa ccataatcca tgattaacgg gaatatacca ggaaagaaac
    33421 agcgcgaggc cgacaatatt caacaacact atttgcggca aatttttaat catattttct
    33481 ctaattatct tactgaaaaa cgcaggcaac cttacgcggt agtcgctaaa cgaagcttca
    33541 acagcgcgct ctgcaaggca ttccagtcag cttcgctgct ggaaatcagc tcaatacggc
    33601 tgtccggtgg cgcaacgttt tgcgtttcaa tgtgcaggtc atcgccctga cggttgattc
    33661 gtaccagccc ttcgggaata cgcagcacgc ctttgacgcg ttccaccggt gcaagtcgcg
    33721 cccattccag aatgccaatg gtgtcgaata ccgaatcagc gtcgaatatc cagccgcagg
    33781 cctgatatcc ttgcccgctg ttcagactgc gacgccagcg ttgatgctct ggcaggctta
    33841 acgctgctaa cccttttttc acgacatgct gatgagaatg cgcggcgctg gcgggcaact
    33901 cggctaaatt gcgacgcggc aaatccagaa gatgaccgtc aactttccca tgctcactgt
    33961 gaattaattg tcgatcgcca ccattttgct gccaccaacg ctgtagcgct tgctcacttt
    34021 cgggcgtcgc acggtcggat ttattggcga caatgatgtc tgcggcagcc agctggtcac
    34081 ggaagttttc attgctggcg cttttttcgt ccagcagcaa gcgcggatcg agaatgcaca
    34141 aggtggcgcg cagatctatc cacggttcat agactggtgc ggttaacaga tcgaggatct
    34201 gtttcggatg gcccagcccg gtcggctcta tcaacaagcg gtctggtttt ccctgacgca
    34261 gtaaggtatt caaccctacc tgcatgggta aaccattaac gcagcacatg cagccgccgg
    34321 ggatctcttt cagcaatgcg ccgctatcgg cgagcaaagc accatcaatt ccgacttccc
    34381 caaattcatt aaccagtacc gcccattttt cgttgggatc tttatgggct aacagatgaa
    34441 gaatcgacgt ggttttcccg ctgccgagaa aaccggtgat gaggttggtc ctggtcacaa
    34501 cggctccgca atactctggt aagttaactg agcgttaatc ctggctaaaa aaggcagaaa
    34561 agagaagggg agagcggacg aaggtgagct tcatccgctt taaccgcaga aatcgttaaa
    34621 ttttgagggt gtttaatagc gcctgatgtg cgccgaattg tacgatttgt tgccaggctt
    34681 gttcgatggc ctgcacaaaa tgcgggttat ctggcagatc atcaccgaaa acttcacgca
    34741 gggaaagcag ggcggttacg cgttgttcac tgctgctgcc cgcaacaagt tcgcgaattt
    34801 tatcgctaag cggatcgcga acatcaatgg catttccggc atcatcaacg ccgctgacgt
    34861 aacgcatcca gccagcaacg cctaatgcca gcaacgacca gtccgtttca cgccccagat
    34921 gtatgcgaat acctgccagc atgcgttgcg gtaatttctg gctgccatcc atcgcgattt
    34981 gccaggtctt atgtttcagc gacggattag caaaacgtgc aattaactta tccgcatatt
    35041 gtgttaaatc gacatcttta atttgcagtg tcggcgcttg ctcatccagc attaatgttc
    35101 tggcagcatg gcgaaatacg cgatcctgca tgcaatcact gatatgggcg aatcctgaga
    35161 ggtaacccag ataagcgaga aaagagtggc tgccattaag catacgcagt ttcatctctt
    35221 cccatggcag gacatcattc accatttgta cacctgcgac ttcccaggca ggacgcccag
    35281 cgatgaagtt atcttcaacc acccactgga taaacggttc gcagctaatc gcgcagggat
    35341 catttacccc cagatgctgg ctgatttctg ccagtgattc gtcggttgca gccggaacaa
    35401 tgcggtcgac catggttccc ggaaaactga cgtgttcttt tatccacccg gcgagttctg
    35461 gcgaacgttt ttctgccatt cccagcaccg cgtttttcac cacatggcca ttgtcgggaa
    35521 tattgtcgca ggagagcacg gtaaacggtg taaggccgcg ctcacggcgg cgtttcagtg
    35581 cttcgacgag aatacccggt gcggagtgag gttcttcagg ggtttgtaga tcgtgaataa
    35641 tccgcggatt actggtgtcg agtgcaccgg tggccgggtc aatacaatag cctttttcgg
    35701 taatcgtcag ggaaacaatt gccacctgtg gctcgcaaaa tttctcaata attgccgcta
    35761 aggaatccag ttttgcatta aggcattcgt gaacggcacc gacaattatc acctgattgc
    35821 cgtccgcacc tttctccagc acggtatata aatggttctg tgcgcggagc tggctcatca
    35881 gttgatcacc gctgaacaag ctgatttcac agatccccca gtcaccgccc tgggcattca
    35941 gcacacgatc ggtcagtaac gcctgatgag cgcggtgaaa ggcgccaaag ccaaaatgaa
    36001 cgatacgtga ttgcaactgc tggcgatcgt agcgtggagc gtgtacatga tgcgggagcg
    36061 taacggaggc aattgtcttc ataggagtca cgtcttagta acgataaatt aacaatgaca
    36121 gtgtaaagtt atattacagc aattgaaatt ggtctggatt gttaatgcga tgaagtgtga
    36181 gggcgatcaa tcaacgagtg aaaattgagg gcgtaaggtg ttcaggaagc gcaaaataga
    36241 agattggtat aataaattaa aaaagggaca gcctgagcta tcccttttct gcatgcggct
    36301 gaagttagtc agcacgcccc atataacggc gttcttcgat atggatacga attttttcgc
    36361 ccgggcttaa gtattctggt acctgaatca ccagaccagt gctcaatgtc gccggtttgt
    36421 tacgggcgct ggcagatgcc cctttgatgc ctggtgcggt ttcaacgatt tccagatcaa
    36481 cggtctgcgg cagctcaagc gccagcagtt gaccgtccca ggtcagcacc tgcatgtccg
    36541 gcatgccgcc ctctggcata aacagcaact cttcttcaat ctgatctttg gtgaaggtat
    36601 acggggtata gtcttcttta tccataaaga catattcgtt gccatcgaca taggagaagt
    36661 caacgtaacg gcgggtcagc gtcacggtgt caacgatatc atcacctttg aaacgctctt
    36721 ctactttcag cccggtacgg acatcagaaa aacgcatttt gtacagcgta gcggcaccgc
    36781 gggcagtggg cgactgaata tcaatatcct taaccagcag tagtttgctg ttgtaattca
    36841 gtaccatacc ttttttaatt tcgttcgctc ttggcatcga aaaattcctg tagtcgaaaa
    36901 tgtcaaaaat atcgcgacaa agtactcgcg acgggccttt cgggcaagtg gtattcgcac
    36961 ttttgctggt gcaaaaaagg tggtactgtg cgcgctcatc aatccggtgg ttaaccttaa
    37021 gagaacaacg tatggaatgc cgtccgggtt gcggggcgtg ttgcactgcc ccttcaattt
    37081 ccagccctat tcccggtatg ccagatggca agcccgccaa tacgccctgc attcagcttg
    37141 atgaacagca acgctgtaaa attttcacct cgccactgcg accgaaagtc tgtgcaggct
    37201 tacaagccag cgctgaaatg tgcggtaact cgcgtcagca ggccatgacc tggcttatcg
    37261 atctggaaat gctgaccgcg ccttaaacat ctttaatccg cagtaagcaa aacagagtgg
    37321 cgataatcat caccatcgca aaccagaaca cagcgtgata attccagatc tcggcgacga
    37381 tgcccgccac tgatcctgcg atgatccagc ccacgcgcga agtgttggta tagagcgtgg
    37441 tggctgaacc cgcctgaccg ggcatcagat cctgaaaata gagcatcccg atgccgccca
    37501 gaatgccaat aaaattagca tttagcagct gcaagcccaa cagaatgaca ggtgaatgcg
    37561 ccatcagcat tcctgcgtaa aaacagacgc cacccacggc agcaacgcgc attaagaaac
    37621 gcttacccag acgtttggcg aaatatccgg caatcaacat cgtcgggatt tccagcccgg
    37681 cggcggtccc catcatcaca ccggccagtt tctcgggaag atgcagttcg ttgataataa
    37741 atagcggcat gttgatgatg tacaggctgt tcgagcccca cattaatgta caaatgacaa
    37801 acagcagtag cgtatcgcga cggttacggc gcggcgcttc aaccgtgccg gtcgccagcg
    37861 gaagctcttt ttgcatcgac ggtaaaaaca gccacaccat cacgccgcaa acaataaacg
    37921 ctaccgctgc gctcagatac attaccgtaa agctgaaacc catcgctaac gcataagcca
    37981 gcggtgggcc aatgacccat gccagtgaaa cctgagcgcg taaaaaagag ctgaacatca
    38041 ccgcctcacg tccggttttg tcggcatgtt cacgggcaag ggcaaacatt tgcgggttag
    38101 cggtcgagcc aaagctgcta agaaagacgc caacgaatag caaaacaaag tagttgcgat
    38161 tccaggcaaa aagggtgcag gccagcacgc ctaacaggca gcaaaagaca atcagcgatt
    38221 tgcgatcgcc gcgcttatca gagcgcccgg cgagaaactg acttaccaga atcccaatga
    38281 cagcgctgcc ggtaaagaag aatcccacca tcgccggacg ggcatgtact tcatcggtaa
    38341 gaaaaatact gagtgtcggg gtttgcagag cgcccgcaat accggtgaga aaggcaacga
    38401 ttaaaaacgc cgtcgaggtc aggtcaaacg atttcgcgct ggagactgcg ggggagttat
    38461 gcatgctggt cgatcactgg aagaggaaga cgcggagttt acgctgcttg tcagaaaata
    38521 aacagacagc gaaatcaaaa gcgccacgaa taatcaaaat gccatttcac tgttgctgat
    38581 tggctgctga cgctgctgaa atgaaattgc tgataacgga ttttcccatc agcaattagg
    38641 aaaaatggca aaaaattgtg cagcacatca aacttttgct cataacttta cggctttcct
    38701 tgcgtgctga aacagaagta ttatgctttc ttgaaacgtt tcagcgcgat cttgtcttta
    38761 accctaagcc aggttggcgc tttttttctc atagaggctg aatcgtttca attcagcaag
    38821 agaggagaac tatgttccag ttatccgtac aggacatcca tccgggcgaa aaggccggag
    38881 acaaagaaga ggcgattcgc cagctcgctg cggcgctggt gcaggccggt aatgtagcag
    38941 aaggctacgt caatggcatg ctggcgcgcg aacagcaaac ctcaacgttc ctcggcaatg
    39001 gtattgctat tccacacggc actaccgaca cccgcgatca ggtgctgaaa accggcgttc
    39061 aggtatttca gttcccggaa ggcgtcacct ggggtgacgg tcaggtagcg tacgtggcaa
    39121 tcggtattgc tgccagctcg gatgaacatc ttggcctgct acgccagctg acccacgtac
    39181 tgagcgatga ttccgttgct gaacaactga agtcagcaac aacagcagaa gaacttcgcg
    39241 cattactgat gggcgaaaag cagagtgagc agctgaagct cgacaacgaa atgctgacgc
    39301 tggatatcgt cgccagcgat ctgctgactc ttcaggcgct gaacgctgcg cgtctgaaag
    39361 aggcgggggc agttgacgcc actttcgtca ccaaagccat caatgaacaa ccgctgaatc
    39421 tcggacaggg tatctggctg agcgatagcg ccgaaggcaa tctgcgtagc gcgattgcgg
    39481 taagccgtgc ggcaaatgct tttgatgtgg acggcgaaac ggcagccatg ctggtgagtg
    39541 tggcgatgaa tgacgatcag cccatcgcgg ttcttaagcg tctcgctgat ttattgctcg
    39601 acaataaagc tgaccgcttg ctgaaagcgg atgcggcaac gttgctggcg ctgctgacca
    39661 gcgatgatgc gccgaccgac gacgtgttaa gcgcggagtt tgtggtgcgc aatgaacacg
    39721 gcctgcatgc tcgtccaggt accatgctgg tcaataccat taaacaattt aacagtgata
    39781 ttaccgtgac aaaccttgat ggtactggca aaccggcaaa cggacgtagt ctgatgaaag
    39841 ttgtggcact tggcgttaag aaaggtcatc gcctacgctt taccgcccag ggtgcagatg
    39901 ctgaacaggc gctgaaagca atcggcgacg ctatcgctgc tggtcttggg gagggcgcat
    39961 aatgagcaga cgtgttgcta ctatcaccct taatccggct tatgaccttg ttggtttctg
    40021 cccggaaatt gaacgcggcg aagtgaacct ggtgaaaacc accggtctgc atgcggcggg
    40081 taaaggcatc aacgtggcca aagtattaaa agacctggga attgatgtca ccgttggcgg
    40141 cttcctgggt aaagacaatc aggatggttt tcagcaactg ttcagcgagc tgggcattgc
    40201 caaccgtttc caggttgtac aggggcgcac ccgaattaac gttaagctga cggaaaaaga
    40261 cggcgaagtg accgacttca acttctcggg ttttgaagtc acccccgccg actgggaacg
    40321 ctttgtgact gattctctga gctggctcgg tcagttcgat atggtctgtg tcagcggaag
    40381 cttaccgtca ggcgtcagcc cggaagcgtt caccgactgg atgactcgcc tgcgtagtca
    40441 gtgtccttgc attatctttg atagtagccg tgaagcgtta gtagcaggtt tgaaagcggc
    40501 accgtggctg gtgaaaccta accgccgcga gttggaaatc tgggcaggcc gtaaactgcc
    40561 tgaaatgaaa gatgtgattg aagctgcaca tgcgctacgt gaacaaggta ttgcgcatgt
    40621 tgttatttca ctgggtgccg aaggcgcgct ttgggttaat gcctccggcg aatggatcgc
    40681 caaaccaccg tcagtcgatg tcgtaagcac cgttggcgca ggggattcta tggttggtgg
    40741 cctgatttat ggcttgctga tgcgtgaatc cagtgaacac acactgcgtc tggcgacagc
    40801 tgttgcagcc ctggcggtaa gtcaaagcaa tgtgggtatt accgatcgtc cgcagttggc
    40861 cgcaatgatg gcgcgcgtcg acttacaacc ttttaactga cagcaggaga ggcataatga
    40921 aaacgctgct gattattgac gctaatctcg gtcaggcacg cgcctatatg gcgaagaccc
    40981 tgctgggcgc ggcggcgcga aaagcaaaac tggaaatcat cgacaatccg aacgacgcag
    41041 aaatggcgat tgttctcggt gattccatcc cgaatgacag cgcgctgaac ggtaaaaatg
    41101 tctggctggg tgatatttcc cgggcagttg cgcaccctga gctgttcctg agtgaagcca
    41161 aaggccatgc gaaaccttac actgcgccgg tcgctgcgac agcaccggtt gctgccagcg
    41221 gtccgaaacg cgtagttgcg gtgactgctt gcccgactgg cgtagcacac acctttatgg
    41281 cggctgaagc cattgaaacc gaagcgaaaa aacgtggctg gtgggtgaaa gttgaaaccc
    41341 gtggttctgt tggcgcgggc aatgcaatca cccctgaaga agtcgcagca gcggatctgg
    41401 tgattgtggc ggcagatatc gaagtggatc tggcgaaatt tgctggtaaa ccgatgtatc
    41461 gcacctctac cggtctggcg ctgaagaaaa ctgcgcagga actggataaa gcggttgctg
    41521 aagcaacgcc gtatgaacca gcgggcaaag ctcaaacagc gaccactgaa ggtaagaaag
    41581 agagtgcagg cgcttaccgt cacttgctga cgggcgtctc ttacatgctg ccgatggttg
    41641 ttgcaggtgg tctgtgtatc gcgctttctt ttgcttttgg tatcgaagcg tttaaagagc
    41701 cgggtacgtt ggcagcggcg ctgatgcaga ttggtggtgg ttcagccttt gcgctgatgg
    41761 tgccggtact ggcaggttat attgccttct ccattgccga tcgtccgggc ctcacaccgg
    41821 gtctgattgg cggtatgctg gcggtcagca ccggttctgg ctttattggc ggtataattg
    41881 ctggcttcct ggctggttac attgcgaagt taatcagtac gcaattgaaa ctgccacaga
    41941 gtatggaggc gctgaaaccg atcctgatca ttcctctaat ttccagtctg gtggtcggtc
    42001 tggcgatgat ctacctaatc ggtaaaccgg ttgctggcat tctcgatggg ttgactcact
    42061 ggctgcagac catggggact gcgaatgcgg ttctgctggg ggcgatcctc ggtggcatga
    42121 tgtgtactga catgggcggt ccggtaaaca aagcagcgta cgcattcggt gtgggtctgc
    42181 tgagtactca aacctatggc ccgatggcgg cgattatggc ggcaggtatg gtgccaccgc
    42241 tggcaatggg tctggcaaca atggtggcgc gtcgcaaatt cgacaaagcg cagcaggaag
    42301 gtggcaaagc cgctctggta cttggtctgt gctttatttc ggaaggtgca attccgttcg
    42361 ctgctcgtga tccgatgcgt gtgctaccgt gctgtatcgt gggtggggcg ctgactggcg
    42421 caatctcaat ggcgattggt gcgaaactga tggcaccaca cggtggtctg tttgttctgc
    42481 tgatccctgg cgctattacg ccggtattgg gttacctggt agcaattatt gccggtacgc
    42541 tggtggcggg tttggcctat gccttcctga aacgtccgga agtggacgca gtagcgaaag
    42601 cagcgtaata aaaggtgtgt tacagggcag aaatttcctg ccctgtaaca tttgatgagc
    42661 gcaaagagaa tgaccagctt gtgaatgtaa ttttatgaat ttaaatggtt ttatctttca
    42721 gtagaaactt caaacgttca tcaaacacta aggttatttc ctcaagggca gattattttc
    42781 tgcccttttt tattgttcct gttctgtgca tcccgtcaca aattcctcgc ttttgtaatt
    42841 caggcattta atcgcatttt gtgagcctgc tcaaatctgc ccattttgcc tctctggcgg
    42901 taatttactt tacgtaaaac atgcatacta tgagcacatg tttaaatatg aacacatgtt
    42961 taaaccgtct ttgcagtatc tatattcttg tggattgaca gggttggtaa ttttttacca
    43021 gaggacgtgc tatgcgcgaa aaggattatg tcgtaattat aggctcggcg aatattgatg
    43081 tcgccggata ttcacatgaa tcattaaatt atgcggattc aaatccaggt aaaataaaat
    43141 ttacgcctgg tggagtcggg cgcaatattg cacaaaacct ggcgttgctg gggaacaaag
    43201 cctggctact gagcgcagta ggcagtgact tttatggtca atcgctgcta acgcaaacca
    43261 atcaatctgg cgtttatgtc gataaatgcc tgattgtgcc gggagaaaat acgtctagtt
    43321 atttatcatt actcgataat accggtgaaa tgctggttgc tataaatgac atgaatatta
    43381 gcaacgctat tacagctgaa tatctcgcac agcaccgtga atttattcag agggcaaagg
    43441 tcattgtcgc ggactgtaat atcagtgaag aggcactggc atggattctg gataatgccg
    43501 ccaacgtacc cgtatttgtc gatccggttt ccgcatggaa atgtgtcaaa gtgcgcgacc
    43561 gtctaaatca gatccacact ctgaagccaa accgccttga agcggaaacc ctgagtggga
    43621 ttgcgctgtc agggcgtgac gatgtggcaa aagttgctgc ctggttccat caacatggcc
    43681 tgaaccgact ggtattgagc atgggcggcg acggcgttta ttacagcgat atcagcggtg
    43741 aaaatggctg gtctgcgccg atcaaaacca atgttattaa tgttaccgga gcgggcgatg
    43801 ccatgatggc gggacttgct tcgtgttggg tagacggaat gccgtttgcc gaatctgttc
    43861 gtttcgcaca gggatgttcg tcaatggcgc tctcctgtga atacaccaat aaccccgatt
    43921 tatcgattgc caacgttata tcgttagtgg agaacgcaga atgtctaaat taaaaatttc
    43981 ccctgaatta ttacaaattt ccccggaagt gcaggaagct ttaaaaaaca aaaaaccggt
    44041 tgtggcgctg gaatcgacca ttatttctca cgggatgccg ttcccacaaa atgcccagac
    44101 cgcaattgaa gtagaagaaa ctattcgtaa acagggcgca gtacctgcca ctatcgccat
    44161 tattggcggc gtgatgaaag tgggtttaag caaagaagaa attgaattac tgggtcgtga
    44221 agggcataac gtgaccaaag ttagtcgtcg cgatttacct tttgttgttg ccgccggaaa
    44281 aaatggcgca accactgtgg cttcaacgat gattattgcg gcgcttgccg gaattaaagt
    44341 atttgccacc gggggaattg gtggtgtgca tcgcggggcg gaacatacct tcgatatttc
    44401 tgccgatttg caagaactgg caaatactaa tgtcaccgtt gtttgtgccg gggcgaaatc
    44461 tattctcgat ttaggattaa ccactgagta tttagaaacc ttcggtgtgc cgttaattgg
    44521 ctatcagact aaagcgctgc ctgcgttttt ctgccgtacc agctcgtttg acgtcagcat
    44581 tcgtctcgac agcgccagtg aaattgcccg tgcaatggcg gtgaaatggc aaagcgggct
    44641 gaacggtggc ctcgtggtag cgaacccgat cccggaacag tttgcgatgc cggaggaatc
    44701 tatcaatgca gccatagatc aagccgtcgc cgaagccgaa gagcagggcg ttattggtaa
    44761 agaaagtaca ccgttcctgc tggcgcgcgt tgctgaactg accggcggtg atagcctgaa
    44821 atccaatatc cagctggtgt tcaacaacgc cattctggcg agtgaaattg ccaaagaata
    44881 ccagcgtctc gcgggttaat ttctgacggg cagaaagccg tcgcccgtac tcatcaaaca
    44941 taccaaaacc tggcgttgac gccgggttta ctggcatgaa gggaaaatca ttatggacat
    45001 aatgagaagt gttgtgggga tggtggtgtt actggcaata gcatttctgt tgtcagtgaa
    45061 taaaaagagc atcagtttgc gcacggttgg agccgcactg ctgctgcaaa tcgctattgg
    45121 tggcatcatg ctctacttcc caccgggaaa gtgggcagta gaacaggcgg cattaggcgt
    45181 tcataaagtg atgtcttaca gtgatgccgg tagcgccttc atttttggtt cgctggttgg
    45241 gccgaaaatg gatgtcctgt ttgacggtgc gggttttatc ttcgcctttc gcgtacttcc
    45301 ggcgattatt ttcgttactg cgctcatcag tctgctgtac tacattggcg tgatggggct
    45361 gctgattcgc atccttggca gcattttcca gaaagctctt aacatcagca aaatcgaatc
    45421 ttttgtcgca gtcacaacta ttttcctcgg gcaaaatgag atcccggcga tcgttaaacc
    45481 gtttatcgat cgcatgaatc gcaacgagtt gtttaccgca atttgtagcg ggatggcgtc
    45541 cattgctggt tcgatgatga ttggttatgc cggaatgggc gtaccaattg actacctgtt
    45601 agcggcatcg ctgatggcga tcccaggtgg tattttgttt gcacgtattc ttagcccggc
    45661 caccgagcct tcgcaggtca catttgaaaa tctgtcgttc agcgaaacgc cgccaaaaag
    45721 ctttatcgaa gcggcggcga gcggtgcgat gaccgggctg aaaatcgccg ctggtgtagc
    45781 gacggtggta atggcgtttg tcgcaattat tgcgctgatc aacggcatta tcggcggaat
    45841 tggtggctgg tttggtttcg ccaatgcctc tctggaaagt atttttggct atgtgctggc
    45901 accgctggcg tggatcatgg gtgtggactg gagtgatgct aatcttgcgg gtagcctgat
    45961 tgggcagaaa ctggcgatta acgaattcgt cgcttacctg agtttctccc catacctgca
    46021 aacgggcggc acgctggaag tgaaaaccat tgcgattatc tcctttgcgc tttgtggttt
    46081 tgctaacttt ggttctatcg gtgttgtcgt tggcgcattt tcggctattt cgccaaaacg
    46141 cgcgccggaa atcgcccagc ttggtttacg ggcgctggca gcagcaacgc tttccaacct
    46201 gatgagtgcg actattgccg ggttctttat tggtttagct tgagtttcat tgccggatgt
    46261 acgcgcttat taagcgttgc atccggcaat cgcgctattg caaataattt ttactgcatc
    46321 atcccggaga atttattctc cgggtccatc tccagcgcca gtcctgacaa ctgctttctg
    46381 tttttaatta gatatccttt cttgctttta attaataaac cgtcgtgaat gaattgcgcg
    46441 agaacatata aaagatgtcg ataagaaacg cctaaatact ctgctgcctg ggtatgtttt
    46501 tcgtgataaa gatcaccctc ctgcgagagt aatataaatg ctgtcaggcg attcactaac
    46561 ggaaatgatt gattctgagt taaagaaaca atattacgat aatttttatg acttaaggtg
    46621 acgcagagtt ttcgtaaaaa tagcgtgtcg tttaataaca gtggacggta atgtttcata
    46681 gggagcgcaa ggcaccaaca ctcttcaata gcctgcaccg cacgcggttc atggtcttta
    46741 tcgattaact caatctcgcc aataaaacag ggggcggcaa agaaatcgat cagcgacacg
    46801 cgaccattag ctagcgtggc gtaaagcctg gcgcggcctc gcgtcaggta aaacagccag
    46861 gacggctgtt gcccttcctg cacaatgtag tcacgcgcta aaaaatgaaa cagccgcgta
    46921 tcggctgaaa catccgttaa aaagcaatcc ttaaacgcgg attcactcat caattgctgc
    46981 ttaagatcat tattgtggat ttctttcacg atgtttatcc gctatgagat ttctcatatt
    47041 tagcatacgt acgctgttgt taagattcaa tcatcggccc ggtcgaattt tctttgctgt
    47101 agaaaaaata acgcaactgg aaacagagga aataaacaat ggaaaagaga aaaattattc
    47161 tggattgtga tccgggtcat gatgatgcta ttgctataat gatggcggcg aaacatccgg
    47221 cgattgattt attaggtatc accattgttg cgggtaatca aaccctcgat aaaacattaa
    47281 ttaatggcct gaatgtttgc cagaaactgg agattaatgt tccggtttat gcggggatgc
    47341 cgcaacccat tatgcgtaaa caaatcgttg ccgataatat tcacggtgaa accggactgg
    47401 atggcccggt attcgagtcg ctgacccgcc aggcagaaag tacccacgcg gtgaaataca
    47461 tcattgatac cctgatggca agcgatggcg atatcactct ggtgccggtt ggtccgcttt
    47521 caaatatcgc ggtggcaatg cgtatgcaac ccgcgatcct gcccaaaatc cgtgaaattg
    47581 tgctaatggg cggcgcttac ggtacaggca acttcacgcc atctgccgag ttcaacatct
    47641 ttgctgatcc ggaagcagcc cgcgtggtgt tcacctccgg cgttccatta gtgatgatgg
    47701 gcctcgatct caccaaccag accgtttgca ccccggacgt gattgcacgg atggaaaggg
    47761 caggcggccc tgccggagag ctgttcagcg acatcatgaa cttcactctc aaaacgcagt
    47821 tcgaaaacta cggcctggct ggcggcccgg tgcacgacgc cacctgcatc ggttatctga
    47881 ttaaccctga tggcattaaa acccaggaga tgtacgtcga agtggacgtc aacagtggcc
    47941 cttgctatgg gcgtaccgtc tgcgacgagc tgggcgttct tggcaagccc gctaatacca
    48001 aagtcggcat cactattgat accgactggt tctggggctt agtcgaagag tgcgtgcgcg
    48061 gctacatcaa aacccattaa gtgttgataa caagccgggt cagtatcgca agatccaagc
    48121 ccggctattt ctgatgggca gaaagccgtc gcccaatatg acatgaagag ataataacta
    48181 tggatgtcat gagaagtgtt ctgggaatgg tggtattgct ggcaattgcg tttttactgt
    48241 cagtaaacaa gaagaagatc agcctgcgta ccgttggcgc ggcgttagtg ttacaggtcg
    48301 tgattggcgg gattatgctt tggttaccgc cagggcgttg ggtcgcagaa aaagtcgctt
    48361 ttggcgtcca caaagtgatg gcgtacagcg acgcaggtag cgcatttatc ttcggttctc
    48421 tggtcggacc gaaaatggat accttatttg atggcgcagg atttatcttt ggtttcaggg
    48481 tattaccggc aattatcttc gtcactgcac tggtgagtat tctctactac atcggtgtga
    48541 tggggatctt aattcgcatt ctcggcggta tcttccagaa agctttaaat atcagcaaga
    48601 tcgagtcatt cgtcgcggtc accaccattt tcctcgggca aaacgaaatt ccggcgatcg
    48661 tcaaaccctt tatcgatcgt ctgaatcgca atgaattatt tacagcgatt tgtagtggca
    48721 tggcctcgat tgctggttcg acaatgattg gttacgccgc actgggcgta cctgtggaat
    48781 atttgctggc ggcatcgtta atggcgatcc ctggcgggat cttgtttgcc cgcctgttaa
    48841 gcccggctac ggaatcttcg caggtttctt ttaataacct ctctttcacc gaaacaccgc
    48901 caaaaagcat tattgaagcc gctgcgacag gggcaatgac cgggctgaaa atcgccgccg
    48961 gtgtagcgac agttgttatg gcatttgtcg ccatcattgc gttaattaac ggtattatcg
    49021 gcggcgttgg cggctggttt ggttttgaac atgcctcgct ggagtccatt ttaggttacc
    49081 tgctggcccc acttgcgtgg gtaatgggtg ttgactggag tgatgcaaat cttaccggga
    49141 gtttgattgg acagaaactg gcaataaatg aatttgtcgc ttatctcaat ttctcgccat
    49201 atctgcaaac gggtggcact ctggatgcta aaaccgtggc gattatttct ttcgcgttgt
    49261 gcggtttcgc taactttggt tctatcgggg tggtggtggg ggcgttttct gcggttgcgc
    49321 cacaccgtgc gccggaaatc gcccaacttg gtttacgcgc gctggcggcg gcgacacttt
    49381 ctaacctgat gagtgctact attgcaggat tctttattgg tttagcgtag tgcgcaattt
    49441 atgagccgga taagatgcgg tcaagcgtcg aatccggcaa cattgttaca ccattggcac
    49501 taatgaaagc gcgttatcgg cagacagggt agggttgttt aagctgccgc tggcgcgcga
    49561 aattgccgcg caggctaccg caaaacgggc gctgtcgcgg aaactgtatc cttcaagaaa
    49621 gctatatacc aggcccgcca taaaaccatc gtcagcacca aaactgtcta ctgtcgtatg
    49681 cgctggcgcg gtcagcaaaa attgttctcc atccttttcg ctgcaataaa ctgactcatc
    49741 gggcaaataa acaaacagtt gctgaacacc ttgctgatgc aatgcattca ctgcggcatt
    49801 acggtcagca tcgctggtga tcgcctgtcc ccataaaatt tccagctccg gtaaagtggg
    49861 tttcagggtg tgaatatgcg ccagccagtg tttgattttg cccgctttga attctgaaac
    49921 ggtatcgaca aacaccggga tttcatcagc gagggtaaag acccattcca gcgcctcggc
    49981 tgtcaggttg caatcagcca gtaccacgcc cgcatgacga agtaaatcgc gcgacccgtt
    50041 cagtagttgc ggtgtcaact gttccagcag atgggtatcg ttaatcgcca gcacggtttg
    50101 atcgtctcga ttggcaattg ccagatacgt cgatgtgctt tgaccatgca aacgaacgca
    50161 gccggagaca ttcacgccgg cacggcgcgt ttcttccagg agcatttcgc cataaaagtc
    50221 atcgccaatc actgaaagca aatggacgtc acggcctaac agcgccagat tgtgggcgat
    50281 gttgcgtccc acgccgcccg ccgagcaatg aattgtaccg ggatgagaag ccgcttgcgg
    50341 gtaacggata tccgccatcc cgcgaatatc catattgatt gtccccacca ctacgcagta
    50401 ttcctgctcg gtgagaatgt aacctttgcc tttaatccgc cctttgcgca ttaaatccat
    50461 aatatgcgcc gcaacgcgcg aacgactgat ttgcagcatg tccgcaattt cgttctgctg
    50521 aatcagcggg ttacgccgta aaattgcaag gatctccttt tcccggttat tcatcttcag
    50581 gctaccgctt tttcagtttg ttgcgctttc agccaggcga tctcttctgc ccagatatcc
    50641 gggttgatgg tttcaaggat cagcggaatg ccgtcgaaac ggtcgtcctg catgatccag
    50701 cggaacgcat catgaccgat attgccttca ccgaggctat gatggcggtc aacgcggctg
    50761 ccaaaggtgc ttttcgcatc gttaaggtgc atcccgcgca gatacttaaa gccaacaata
    50821 cgggcaaaat ccgcgaacgt tttctcgcat tcggctggag tacgcaaatc atacccggca
    50881 gcgaaagcat ggcaggtatc aatgcagacg ccgacgcggg atttatcttc cacgccgtcg
    50941 ataatcgccg cgagatgttc gaatttaaac cctaagttac tgccctgacc ggcggtgttt
    51001 tcaatcaccg ctgttacacc ttgagtttta tccagcgcaa tgttgatgga ttcggcaata
    51061 cgcgcaaggc aatcctcttc tgaaatctgc atcagatggc tgccagggtg gaagttgagc
    51121 aaagaaagcc ccagttgttc gcaacgctgc atttcatcta taaaggcatc gcgcgatttt
    51181 tccagagctt cagtgaccgg atgtccgagg ttaatcagat aactgtcgtg gggaagaatt
    51241 tgcgccgatg tgtagtgata tttttcacag gcggctttga attcatcgat ggtttgcgtc
    51301 gtgagcggtg cggcacgcca ctgacgttgg tttttggtga acaaggcaaa cgcggttgcg
    51361 tcgatttcgg cggcgcgaat tgcggcattt gccagaccgc cagcagcact aacgtgcgct
    51421 ccaatgtatt tcatgcgagg actcctgtta aacccgctgg aggaaaacgg taatgatagc
    51481 gggttaacag gagcaagatg tagtggttta tgcgatgacg ctttgaatca catagttaat
    51541 cgcaccacca ccaacaatca gccaggcaaa cagtaccagt gccatcagca gaggtttcgc
    51601 cccagctttt ttcagcgcgc tgacgtgagt ggtcagaccc agcgccgcca tcgccattgc
    51661 cagcaggaag gtatccagcg ttaccagcat gttcaccacg ctctgcggta acaggtggaa
    51721 cgagttaaag atggcaacga cgatgaacaa gatggcaaac cacggaatag tgattttgct
    51781 tttctcgccg ctgttcgccc cagacagctg tttaacacgc gccgccagca ggatgaggaa
    51841 cggagccagc atcatcacgc gcagcatttt ggaaataact gctgcgtttt ccgcatccgg
    51901 gctgatggca tgacctgccg ccaccacctg cgccacttcg tgcacagtag aaccaatgta
    51961 gataccgaaa gtttccggac taaaccattg agacatcagc ggatatatcg ccgggtagag
    52021 gaaaatcgcg acggtcccga agataacaac ggttgcaaca gccacggtta ctttactggc
    52081 ttccgctttc actaccggct cagtcgccag taccgcggca gcaccacaga tactgctacc
    52141 ggcaccgatc aaccagctgg tgtgcttatc cagaccaaac actttctgcc ccaggaagca
    52201 agccagcagg aaggtactgg acagcgtcaa cacgtcaatg atgatcccac tgataccgac
    52261 atcggcaatt tgcgagaacg tcagacggaa gccataaaga atgataccca gacgtaataa
    52321 atattgcttg gcaaacagca caccaccgtc acagcttttc cagatgtgcg gatagatggt
    52381 gttgcctaaa accatcccca acaagattgc gagggtgagg gcactaaacc cggcacccgc
    52441 aaccgcggga atggaaccac cccacagggc gaccccggtg ataactgcac tcagggctaa
    52501 ccccggaata aaatgccaca gtgtacgatg ttgtttctgt aaggtgatat tcgtcataac
    52561 cctctccttt aaccgggcat aaggttacga ctaactggtt taaaaataaa attgattata
    52621 tatttataat taatctttat aagtggtaag cgactatgca catcaccctc cggcagttgg
    52681 aagtttttgc agaagtattg aaaagtggat caaccaccca ggcgtcggtg atgctggcgt
    52741 tgtcgcaatc agcagtgagc gcagccttga ccgacctgga agggcagctt ggcgtgcaac
    52801 tgtttgatcg cgtggggaaa agactggttg ttaatgaaca cgggcggctg ctctatccgc
    52861 gtgcgttggc attgcttgaa caggcggttg aaatcgaaca actgtttcgc gaagacaacg
    52921 gcgcgattcg tatctatgcc agtagtacca tcggtaacta cattctgcct gcagttatcg
    52981 cccgttatcg ccatgattat ccgcagttgc cgattgaact tagcgttggg aacagccagg
    53041 acgtgatgca agcggtgctg gatttccgcg ttgatattgg ctttattgaa ggaccgtgcc
    53101 acagcactga aatcatttct gaaccgtggc tggaagacga gctggtggtt ttcgccgcgc
    53161 cgacttcgcc gttggctcgt ggtccggtca ccttagaaca gctggccgct gcgccgtgga
    53221 tcctgcgtga acgcggttcc ggcacgcggg agattgtcga ttatctattg ctgtcacatt
    53281 taccgaagtt tgagatggcg atggaattag gtaactccga ggcaatcaaa catgcggtgc
    53341 gtcatgggtt gggaattagt tgcctgtcgc gacgggtgat tgaagatcaa ttgcaggcag
    53401 gcacattaag tgaagttgcg gtccctctgc cgcgcctgat gcgtacgttg tggcgtatac
    53461 atcatcggca aaaacacctt tccaacgcgc tacggcgctt tctggactat tgcgatcccg
    53521 cgaatgtacc gcgttaaggt gctgtacaag aacatgctgg tgctgtgtcg atttcgtgac
    53581 gcagcgccct cagcatgcat tcgccagaaa agagattggc tgctttactt ataatccctg
    53641 ggcgatcatg aaggtgtctt ataaccgtgt atttctgccg gaaggattgc caatcgtctg
    53701 ctacaatcgc gcctcatttt taagatggat agcatttttg tatggtttcc gaaactaaaa
    53761 ccacagaagc gccgggctta cgccgtgaat taaaggcgcg tcacctgacg atgattgcca
    53821 ttggcggttc catcggtaca ggtctttttg ttgcctctgg cgcaacgatt tctcaggcag
    53881 gtccgggcgg ggcattgctc tcgtatatgc tgattggcct gatggtttac ttcctgatga
    53941 ccagtctcgg tgaactggct gcatatatgc cggtttccgg ttcgtttgcc acttacggtc
    54001 agaactatgt tgaagaaggc tttggcttcg cgctgggctg gaactactgg tacaactggg
    54061 cggtgactat cgccgttgac ctggttgcag ctcagctggt catgagctgg tggttcccgg
    54121 atacaccggg ctggatctgg agtgcgttgt tcctcggcgt tatcttcctg ctgaactaca
    54181 tctcagttcg tggctttggt gaagcggaat actggttctc actgatcaaa gtcacgacag
    54241 ttattgtctt tatcatcgtt ggcgtgctga tgattatcgg tatcttcaaa ggcgcgcagc
    54301 ctgcgggctg gagcaactgg acaatcggcg aagcgccgtt tgctggtggt tttgcggcga
    54361 tgatcggcgt agctatgatt gtcggcttct ctttccaggg aaccgagctg atcggtattg
    54421 ctgcaggcga gtccgaagat ccggcgaaaa acattccacg cgcggtacgt caggtgttct
    54481 ggcgaatcct gttgttctat gtgttcgcga tcctgattat cagcctgatt attccgtaca
    54541 ccgatccgag cctgctgcgt aacgatgtta aagacatcag cgttagtccg ttcaccctgg
    54601 tgttccagca cgcgggtctg ctctctgcgg cggcggtgat gaacgcagtt attctgacgg
    54661 cggtgctgtc agcaggtaac tccggtatgt atgcgtctac tcgtatgctg tacaccctgg
    54721 cgtgtgacgg taaagcgccg cgcattttcg ctaaactgtc gcgtggtggc gtgccgcgta
    54781 atgcgctgta tgcgacgacg gtgattgccg gtctgtgctt cctgacctcc atgtttggca
    54841 accagacggt atacctgtgg ctgctgaaca cctccgggat gacgggtttt atcgcctggc
    54901 tggggattgc cattagccac tatcgcttcc gtcgcggtta cgtattgcag ggacacgaca
    54961 ttaacgatct gccgtaccgt tcaggtttct tcccactggg gccgatcttc gcattcattc
    55021 tgtgtctgat tatcactttg ggccagaact acgaagcgtt cctgaaagat accattgact
    55081 ggggcggcgt agcggcaacg tatattggta tcccgctgtt cctgattatt tggttcggct
    55141 acaagctgat taaaggaact cacttcgtac gttacagcga aatgaagttc ccgcagaacg
    55201 ataagaaata agtttcctcc cttccttgct aagccctctc aaccgagagg gctttttcaa
    55261 ttccatttcc ctgacaaatc atgcggatat aaaatttaac atttggattg ataattgtta
    55321 tcgtttgcat tatcgttacg ccgcaatcaa aaaaggctga caaatcagag gctgttccgg
    55381 ctttctggga tgatcacctg cataaaaaat aagtccaccg cgatgctgcc gtacgcaagg
    55441 ggacgtgaag aagatgtgag cgataaccca ttttattttc gtagttacct catggagata
    55501 tggaatgttt aggttgaacc ctttcgtacg ggtcgggctg tgtttgtccg ctatttcttg
    55561 tgcatggcct gtgttagcgg tcgatgatga tggcgaaacg atggttgtca ctgcatcttc
    55621 cgtggaacaa aatcttaaag atgcacctgc cagtatcagc gtcattaccc aggaagacct
    55681 gcagcgaaaa ccggtacaga atctgaagga tgtcctcaaa gaagtgcctg gcgtacaact
    55741 gacgaacgaa ggggataacc gtaagggcgt tagtattcgt ggtctggaca gcagctatac
    55801 cctgattctc gtcgacggta aacgcgtgaa ctcccgcaat gccgtcttcc gccacaatga
    55861 tttcgatctg aactggatcc cggtcgattc catcgaacgt attgaagtgg tccgtggccc
    55921 gatgtcgtcg ctgtacggtt ccgatgcgct cggcggtgta gtgaatatca tcaccaaaaa
    55981 aatcggtcag aaatggtcgg gtaccgttac cgtcgatacc accattcagg aacatcgcga
    56041 tcgcggtgat acctataacg gtcagttctt taccagtgga ccattaattg atggtgtgct
    56101 gggaatgaaa gcttacggca gcctagcaaa acgtgaaaag gatgacccgc aaaactcaac
    56161 gaccaccgat accggagaaa cgccgcgtat tgaaggattc tccagccgcg acggcaatgt
    56221 cgaatttgcc tggacaccga atcaaaatca cgattttact gccggatacg gtttcgaccg
    56281 tcaggatcgt gattccgact cgctggacaa aaaccgcctg gaacgccaga actactccgt
    56341 cagccataat gggcgttggg attacggcac cagcgaactg aaatactacg gtgagaaagt
    56401 cgagaacaaa aaccctggca acagcagccc gataacttcc gaaagcaata cggtcgacgg
    56461 caaatacacg ttgccgctga cggcgattaa tcagtttctc acggttggcg gtgaatggcg
    56521 tcacgacaaa cttagcgatg cggtgaacct gaccggggga accagctcca aaacgtctgc
    56581 cagccagtac gcgctgtttg tggaagatga atggcggatc ttcgagccgc tggcgctgac
    56641 gaccggcgtg cgtatggacg atcacgaaac ctacggtgaa cactggagtc cgcgtgccta
    56701 cctggtttat aacgccaccg acaccgtaac ggtgaaaggg ggctgggcga cggcatttaa
    56761 agcaccttct ctgttgcaac ttagccctga ctggacgagc aattcctgcc gtggcgcatg
    56821 taagattgtg ggtagcccgg atctgaaacc agaaaccagc gaaagttggg agctggggct
    56881 ttactacatg ggtgaagaag gctggctgga aggggttgaa tccagcgtta ccgttttccg
    56941 taacgatgtg aaagatcgta tcagcatcag ccgtacgtct gacgtcaatg ctgcaccggg
    57001 ctaccaaaac tttgtcggtt ttgagacggg cgctaacgga cggcgcatac cggtatttag
    57061 ctactacaac gttaacaaag ctcgtattca gggcgtggaa accgaactga aaattccgtt
    57121 cagcgatgaa tggaaactgt cgatcaacta cacctacaac gatggtcgtg atgtcagcaa
    57181 cggcgaaaac aaaccgctat ccgatctgcc gttccatacc gctaacggta cgctggactg
    57241 gaaaccgctg gcgctggaag actggtcatt ctatgtttct ggtcactata ccgggcagaa
    57301 acgagccgac agcgcgacgg ctaaaacacc tggcggttat accatctgga ataccggcgc
    57361 ggcctggcag gtgactaaag acgtcaaact gcgcgcaggc gtgctgaacc ttggcgacaa
    57421 ggatctcagt cgtgacgact acagctataa cgaagacgga cgtcgttact ttatggcagt
    57481 ggattatcgc ttctgatgag aagatgcccg gcgaaccggg cggactttca cttcagtaaa
    57541 tactgcgcat ggaagcgcag gtgatcctct ataaaagagg cgatgaagta gtaactgtga
    57601 tcatatcccg gctgaatacg cagcgtcatc ggccacgctt tctggcgcgc ggcttctgcc
    57661 agtaccgcag gttgcaactg gtcggcaaga aactgatcat tatcgccctg atcgataagc
    57721 gtcgggatcg catcctgcgc gttactggca tacatcagcg cgcaactgtc ccattccagc
    57781 catgcatttt tgtcctcacc taaatagctg ctaaacgctt tgattcccca cgggacgctg
    57841 cacggattca caattggcgc aaaggccgaa acgctggtgt atttacccgg atttttcagc
    57901 gccataatca gcgcaccgtg accacccatt gagtgaccgc taatggcgca gcggtcgctg
    57961 acattaaatt gcgactgaac cagcgccggt aattcatcgc gcagataatc atacatccgg
    58021 taatgcgtcg cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc ctggcctaaa
    58081 tcgtagccat cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg catcaccagt
    58141 acaatcccca gttctgccgc cacgcgctgg gcacccgcct tggtggtgaa gttctcgtca
    58201 ttgcaggtta atccggaaag ccagtacagc actggtggcg gggtgtgatc acgagggggt
    58261 gggagaaaga tactgaacgt catcgggcag tttaaggtac tggagtcgtg tcgccagcgt
    58321 tgctgccagc cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg ctccttgttg
    58381 tgttgtttgc aatttcttca tcataccgtt tattccatgt gctgtgagta actttcactt
    58441 ccgtatttgc ataacgatgt tttaacatct gctgatgaaa ggcagcggca attacaataa
    58501 ttatcgctgt gaatactgga ttatgtgcgc cgcctcacgc acaataatca ggctgtaaat
    58561 cagcttaata actttgcccc cacgcagggc ggaggcgtca cacctgcagg agaaatcata
    58621 aatgccatca ctcagtaaag aagcggccct ggttcatgaa gcgttagttg cgcgaggact
    58681 ggaaacaccg ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca aaagccttat
    58741 tgctggtcat atgaccgaaa tcatgcagct gctgaatctc gacctggctg atgacagttt
    58801 gatggaaacg ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct ccggtctgga
    58861 ttacgccaac ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg atgaaatggt
    58921 caccgtgcgc gatatcactc tgaccagtac ctgtgaacac cattttgtta ccatcgatgg
    58981 caaagcgacg gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa aaattaaccg
    59041 tatcgtgcag ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc agcaaattct
    59101 tattgcgcta caaacgctgc tgggcaccaa taacgtggct gtctccattg atgcggtgca
    59161 ttactgcgtg aaagcgcgtg gcatccgcga tgcaaccagt gccacaacaa cgacctctct
    59221 tggtggattg ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg ctgtgcgtca
    59281 tcacaactga ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat tttgttcgcg
    59341 gcgtcgccat tctggggatc ctgctattaa acatcagcgc ctttgggcta ccaaaggcgg
    59401 cttatctcaa tcccgcctgg tacggcgcta ttacgccgca ggatgcatgg acctgggcat
    59461 ttctcgatct catcggccag gtgaaattcc tcacgctttt tgcgctgctg tttggtgcgg
    59521 gcctgcaaat gttgctgccc cgtggcagac gctggatcca gtcgcggtta acgctgttag
    59581 tcttgctggg ttttattcac ggtttattgt tctgggacgg cgatattctg ctggcttacg
    59641 ggctggtggg cttaatctgc tggcggctgg tgcgcgatgc gccatcggta aaaagcctgt
    59701 ttaatacagg cgtcatgctt tatctggtgg ggcttggcgt tttgctgtta ttggggctga
    59761 tttccgacag ccagaccagc cgcgcctgga cgccggatgc atcggctatt ttgtatgaaa
    59821 aatactggaa gcttcacggc ggcgttgaag cgatcagtaa tcgtgccgat ggtgttggca
    59881 acagtttact ggcactgggc gcacagtatg gctggcaact ggcagggatg atgctcattg
    59941 gtgcggcgct gatgcgcagc ggctggctga aagggcagtt cagcttacgt cactatcgtc
    60001 gtactggttt tgtgctggtg gcgattgggg tgaccattaa ccttcctgcc atcgccctgc
    60061 aatggcagct ggactgggcg tatcgctggt gtgccttctt acttcaaatg ccgcgggaac
    60121 tgagtgcgcc gtttcaggcg attggctatg cgtcgctgtt ttatggcttc tggccgcaat
    60181 tgagccgctt taagctggtg cttgcgatcg cctgcgtcgg acggatggcg ctgaccaact
    60241 atctattgca aacgctgatt tgtaccatgc ttttttacca cctcggtttg tttatgcatt
    60301 ttgaccgcct ggagctgctg gcgtttgtta ttccggtatg gctggcgaat atcctcttct
    60361 ctgttatctg gctgcgttac ttccgccagg ggccggtgga atggctctgg cgtcagttaa
    60421 ctttgcgtgc tgccggaacg gcaatatcta aaacatcaag ataacgatct ggatcacatt
    60481 cgttaacaaa acggctgtaa ccgtttccat tgctgtgact cgattcacga agtcctgtat
    60541 tcagtgctga caaaatagcc gccagcaagc agtcatttac tgcaatctca taacaggtag
    60601 tgaatatgat caccattcgt gatgtagcgc gtcaggctgg cgtctctgtg gcaacggttt
    60661 cccgggtgct caataacagc acgctggtca gtgccgacac gcgtgaagca gtaatgaaag
    60721 ccgtgagtga gctggattat cggccaaacg ccaatgccca ggcgctggca actcaggtta
    60781 gcgacaccat tggcgtggtg gtgatggacg tttctgatgc gtttttcggc gcgctggtaa
    60841 aagcggtgga tctagtcgct cagcagcatc agaaatacgt gctaatcggc aatagctatc
    60901 atgaagcgga aaaagagcgt cacgccattg aggtgttaat tcgccagcgt tgtaatgcgt
    60961 tgattgttca ctcaaaagca ttgagtgacg atgaactggc gcaatttatg gataacattc
    61021 ccggtatggt gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc gtttgcctgg
    61081 ataatctcag cggtgcccga atggcgacgc gcatgttgct gaataacggt catcaacgta
    61141 ttggttatct ttcttccagt cacggcattg aagatgacgc catgcgtaaa gcaggctgga
    61201 tgagtgcgtt gaaagagcag gatattattc cgccggaaag ctggattggc actggtacgc
    61261 cggacatgcc gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat ctacaactta
    61321 ccgctatatt tgcttataac gacaatatgg ccgctggcgc actgacagca ttaaaagata
    61381 atggcattgc gattccgtta catctctcaa tcatcggttt cgatgatatt cccatcgccc
    61441 gttacaccga cccgcaatta acgaccgtgc gttatcccat tgcttcaatg gctaaattag
    61501 ccaccgaact ggccttgcag ggggcagcag gcaatattga tcctcgtgcc agccactgtt
    61561 ttatgccgac gttagtgcgt cgccattctg tcgcaacacg ccagaatgcg gcgacgatca
    61621 ctaactcaac aaatcaggcg atgtaaccgc tttcaatctg tgagtgattt cacagtatct
    61681 taacaatgtg atagctatga ttgcaccgtt ttaacgttgt aacccgtatg taacagtgaa
    61741 taatcacttt caccgaggta atagcgtcat aacaacaatt aaagccgttt tctggagcgt
    61801 taccgggcat ggaagaacga attttaaaaa gtgagcttcg gcgttcagta acacttcatt
    61861 aactctactg ccccgccgag catttatctc aagcactacc ctgcataaga aaaaccggag
    61921 ataccatgaa taagaaggtg ttaaccctgt ctgctgtgat ggccagcatg ttattcggtg
    61981 ccgctgcaca cgctgctgat acccgcattg gtgtaacaat ctataagtac gacgataact
    62041 ttatgtctgt agtgcgcaag gctattgagc aagatgcgaa agccgcgcca gatgttcagc
    62101 tgctgatgaa tgattctcag aatgaccagt ccaagcagaa cgatcagatc gacgtattgc
    62161 tggcgaaagg ggtgaaggca ctggcaatca acctggttga cccggcagct gcgggtacgg
    62221 tgattgagaa agcgcgtggg caaaacgtgc cggtggtttt cttcaacaaa gaaccgtctc
    62281 gtaaggcgct ggatagctac gacaaagcct actacgttgg cactgactcc aaagagtccg
    62341 gcattattca gggcgatttg attgctaaac actgggcggc gaatcagggt tgggatctga
    62401 acaaagacgg tcagattcag ttcgtactgc tgaaaggtga accgggccat ccggatgcag
    62461 aagcacgtac cacttacgtg attaaagaat tgaacgataa aggcatcaaa actgaacagt
    62521 tacagttaga taccgcaatg tgggacaccg ctcaggcgaa agataagatg gacgcctggc
    62581 tgtctggccc gaacgccaac aaaatcgaag tggttatcgc caacaacgat gcgatggcaa
    62641 tgggcgcggt agaagcactg aaagcacaca acaagtccag cattccggtg tttggcgtcg
    62701 atgcgctgcc agaagcgctg gcgctggtga aatccggtgc actggcgggc acagtactga
    62761 acgatgctaa caaccaggcg aaagcgacct ttgatctggc gaaaaacctg gccgatggca
    62821 aaggtgcggc tgatggcacc aactggaaaa tcgacaacaa agtggtccgc gtaccttatg
    62881 ttggcgtaga taaagacaac ctggctgaat tcagcaagaa ataagactga ttgggcgcag
    62941 tcgtttactg cgcccttatt gtatggccaa caaggtaaaa ttatatggtc agctcaacga
    63001 ctccgtcttc cggggaatac ttgttggaaa tgagcggtat caacaagtct tttcctggtg
    63061 ttaaggcact tgataacgtt aatttaaaag tccggccaca ttctatccat gcattaatgg
    63121 gggaaaacgg cgcaggaaaa tcaacattat taaaatgcct gtttggtatt tatcaaaaag
    63181 actccggcac cattttattc cagggtaaag agatcgattt ccattctgcc aaagaagcac
    63241 tggaaaatgg tatttcgatg gtacaccagg agttaaacct ggtattacaa cgttcggtga
    63301 tggataacat gtggctgggg cgatatccca ccaaaggcat gtttgtcgat caggacaaaa
    63361 tgtaccgcga aaccaaagcg atttttgatg aactggatat tgatatcgat ccgcgtgcgc
    63421 gcgtcggcac attatccgtt tcgcaaatgc agatgatcga aatcgccaaa gcgttttcct
    63481 ataacgcgaa aattgtgatt atggatgaac cgacttcttc gttaaccgaa aaagaggtca
    63541 atcatctgtt cactattatt cgtaaattaa aagagcgcgg ctgcggtatt gtttatatct
    63601 cgcataaaat ggaagaaatc ttccagttat gtgatgaagt taccgtattg cgcgacggtc
    63661 agtggatcgc caccgaaccg ctggcaggac tgacgatgga caagatcatc gccatgatgg
    63721 ttgggcgttc tcttaatcag cgttttcctg acaaagaaaa caagccgggc gaagtcatcc
    63781 tcgaggtgcg taacctaacg tcactgcgcc agccgtcgat tcgcgatgtc tcgtttgatc
    63841 tgcataaagg ggagatcctc ggtattgccg ggctggtggg ggcgaaacgt accgatattg
    63901 ttgagacgtt atttggtatt cgcgagaaat cggctggcac cattacgttg cacggcaaac
    63961 agatcaataa ccataatgcc aacgaagcca taaaccacgg atttgcactg gtaactgagg
    64021 agcgccgctc aacgggaatt tatgcctatc tggatattgg ttttaactcg ttaatttcca
    64081 atattcgcaa ctacaaaaat aaagttggtt tactggataa ctcgcggatg aaaagcgata
    64141 cccagtgggt gattgattcg atgcgggtaa aaacgccggg tcatcggacg caaattggtt
    64201 cgctctccgg tggtaatcag caaaaggtga ttattggtcg ctggctacta acgcaaccag
    64261 aaatattaat gctcgatgaa ccgacgcgcg gtattgatgt cggggcgaag tttgaaattt
    64321 atcagttaat tgccgaactg gcgaagaaag gcaaggggat tattattatc tcctctgaaa
    64381 tgcctgagtt gttagggata acagaccgta ttctggtcat gagcaatggt ctcgtttccg
    64441 gaattgtcga tacaaaaaca acaacgcaaa acgaaattct gcgtcttgcg tctttgcacc
    64501 tttaagatta ggggcttccc atgagtgcgt taaataagaa aagttttctt acttacctga
    64561 aagagggcgg tatttacgtc gttcttttag ttttgctggc gattattatt ttccaggacc
    64621 caacattttt aagtctgttg aacttaagta atattctcac ccagtcatcg gtgcgtatta
    64681 ttatcgcgct cggtgtggca gggttaattg tcacccaggg gaccgatctt tctgctggtc
    64741 gtcaggtagg gctggcggca gtggtggctg cgacattatt gcagtccatg gataacgcca
    64801 acaaagtgtt cccggaaatg gcgacgatgc cgattgcgct ggttattctg attgtctgtg
    64861 ccattggtgc ggtgatcggt ttgatcaacg gtctgattat cgcttatctc aacgtgacgc
    64921 cgttcattac cacgctcggc acgatgatca tcgtctatgg catcaactcg ctctattacg
    64981 actttgtcgg ggcgtcgcca atttctggtt ttgacagtgg cttctctacc tttgctcagg
    65041 gctttgtcgc gctggggagt ttccgtctct cttacatcac cttctacgcg ttgattgcgg
    65101 tggcgttcgt ctgggtgttg tggaacaaaa cccgcttcgg taagaacatt tttgccattg
    65161 gcggtaaccc ggaagcggca aaagtatctg gtgtcaacgt cggcctgaac ctgctgatga
    65221 tctacgcgtt gtctggcgtg ttctatgcct ttggcgggat gttagaagcc ggacgtatcg
    65281 gctctgccac caacaacctc ggctttatgt atgagctgga tgctatcgcg gcgtgcgtgg
    65341 taggcggtgt atcgttcagc ggcggtgtgg ggacggtgat tggcgtggtg accggggtaa
    65401 ttatttttac cgtcatcaac tatggcctga cgtatatcgg cgtaaaccca tactggcagt
    65461 acatcatcaa aggggcgatt attatcttcg ccgtagcgct ggattcactg aaatacgcgc
    65521 gtaagaaatg atgtgaatgt ggtccgcatt cggtcataaa cgcacataac atcatgatct
    65581 gtgtggtttt gtaggccgga taaggcgttc acgccgcatc cggcaacagg agccaaatgc
    65641 cggatgcaac gctggcgcgt tttatccggc ctacaataag ctcaacatac tgatttgtat
    65701 ggttttgtag gccggataag gcgttcaagc cgcatccggc gacaggaacc aaatgccgga
    65761 tgtgacgcgg gttctacaac gttaccgggt gttctttctc gcctttctta aacttcactt
    65821 ccccgagctc aatacaaccc accgggcaga cgtgaccaca aagcagacaa cccacacatt
    65881 tctcggtatt acaatgcggt gtgcgggttt tctcgctcca ttccatcgcc tggtgaccgc
    65941 cgtcgtaaca ggaaatataa cagcgtccac agccaacaca tttgtcaaga ttgatacggg
    66001 gatagacaat ataactgcgg tctaaatctt ccgccgggac aatattgtta ttcgccagac
    66061 ctaccatctc ctgcaacgaa tcaaatccct gatcggcgag ataatgcgat aacccgctcg
    66121 ccatatcttc tacgatgcga tagccgtact gcataatgcc ggtggtgact tgtaacgttg
    66181 ctgcgccgag caataaaaac tccgccgcat cctcccaggt ttcaatgccg ccgataccgc
    66241 tgattgggaa atcgcgcagt tctggatggg tgcgcatttg ctgaatgaag cgcagggcga
    66301 tcggttttac cgctttaccg gaatatccgg aaatactcga tttaccgtta acgatcggca
    66361 taccgatttt ctgattaaga tcgatattgg tgatggattt aacggtgtta attgccgcaa
    66421 tgccatctgc gccgccgcgc ttcgccgcca gcgccacttc gcacatatca ccgatattcg
    66481 gcgtcatctt cgccagcatt ggcagcgtgg aaccgcgttt caccgcccga caatattttt
    66541 ctaccagcac cggactttgc ccgacatcgc tacccatcgc atgagaagtc atttgcggac
    66601 aggagaagtt acactcgatc atatccgcgc cagcttcttg caccaggcgc gccagctcct
    66661 cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc gggtaatctt
    66721 ctttcagccg acgcagggcg gccagattct cctccagcgg atgctcggca atctgctcca
    66781 tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga
    66841 cttcgttggc gataaaaaag ccgatcgttt taaacaccac accgccccaa cctgtgtcgt
    66901 aggctttggc acacatctca tagcagttgc ctaccggcga agaagagagg cagaacgggt
    66961 tgggaaactt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg agcaagctcc
    67021 ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg catagaccac
    67081 ggttttgtct ccctcaacaa tatcgccagc agcaaagact tgcgggtcgc gggtctggta
    67141 attttgtgtt ttgatggtgt tacgctgcgg ctctaactcc gcaaaggcat ctagtctggc
    67201 atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc ccgataaccg
    67261 tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc catcgatgat
    67321 cgaaacgccc agctcccggg cgctggtaaa ctctttttcg cttgccggaa actcatctaa
    67381 ctcttcacgc gctacgcaag ttaccgcctg acagccgaga actttcagcg tgctggctac
    67441 gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc tgatatcgcc
    67501 ttgcgcctgt cgtacacgtt gcaggaagtc gacggcaatc tcaacgtcac tatgctcaaa
    67561 cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc ggttttctgc
    67621 tttcagctgc tcaagggtga gtgtgttacc gacttcgttg ttgcacttaa tggtcacgcc
    67681 cattttttca atacgggcga tctctgcatc cagcactgat tgcggtaacc ggaattgcgg
    67741 aataccgtta cgcaaccagc caccggggtg cgcttctttc tcataaatcg tgacgtcata
    67801 accctggttt gtcagtgtta cactggcctg caatccggca ggacctgcgc caataatcgc
    67861 gactttgccg agcgttttag taccgggctg ataaatttcc atcccagttt gttgttcaaa
    67921 atcagtaaca aaacgctgta agcgaccgat atcaatggga gtatcaacac cggcacgggt
    67981 acaaccgctt tgacataatt tctccgtcgg gcacactctg gcacaaacgg caccgagggc
    68041 attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat
    68101 aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt catgacataa
    68161 taaacagcga gaggcttctt taatcgccag taaaggcgta aaagccggag tgagttcatc
    68221 cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta aggcgagcgt
    68281 ttagtaaata ataatgtact gatattaagg ctaagcgtgt ggggaaattt aaactgtgaa
    68341 aggattcaca aatagctgcg gttcagaatg ttatatccgc agcttattga gtgaagtaat
    68401 aattattctc aataattaac agaaaagaga aaatattaac gatagaaaat aagataaaca
    68461 atcatgccga tagataaaat tccgaagccg atatatgcca ccatataagc gaaaaagagt
    68521 ccgggatttt ttgctcgcca gacatacacc ggttccggtg catttttaac ccgtttatca
    68581 atcgcgcctg cgcgccagcc tttccatgct tcgcggaaac aaaatatcgg gataaggaaa
    68641 aaaacggcaa cgacaaaaaa ctgctgtaca tccataacta ctttccttgt ttcttttgta
    68701 attcaagtaa ctgttcgggt gtgacggcgg gatacccctg cgcatcttcc ggcacctggt
    68761 gcatagccgg aatagggacc agcggcccta aaaaacgcgg ttcacgttta aaaatataaa
    68821 ggtcagccag cgcaccgaaa cgggcagcaa attcgcgaat acgtactgac agcatatctt
    68881 tcggtgacgg tacggcataa cactgagcct gaatccccat atgcagcgca ataaataatg
    68941 cccgctcaca gtggaaacgt tgggtgataa taatgaaatc attagtatcg aacactttgc
    69001 gtgtacgcac gatggaatcc agcgtacgaa agcctgcgta atcgagaaca atatctgatg
    69061 ggtcgacacc agctgcgatt aaatctttgc gcatggtcat cggctcatta taactttgca
    69121 atgcgttatc gccgctcagt aatagataat ttaccttacc gctgttatag gcattaatcg
    69181 ctccttgaat gcggtagcga taatactgat taattacgcc agtacgataa tattttgctg
    69241 ttccgagcac cacaccgacc tggcggtagg ggagatcctg caattcgtcg tagatataag
    69301 gcgcggtttt ccagctcatc cagcgatcga ggccgagcac agtcaacagc agcaagccga
    69361 tcaggactaa caggctgagg aacacgcgct ttaacatgca ggagactcga tctaaggaaa
    69421 atttccatca ggctacttta cccgctggtt ttgcgcaaga aacggcaaac tgtttgccgg
    69481 gaatttcgac accaggagag ggctggcaag ccggacaagg cgttcacgcc gcatccggca
    69541 ccaggcttaa gcgagaagca ctcggtcgat actgtgacag ccgagagctt tcagcgttgc
    69601 ggaggtggca tcccactgaa tcaacggcgc atcggctttt tctgccagaa ccgcgcgctg
    69661 gatatccggg taatcataac ccttgagatt caacagaatt aacgctccct gcaacggtgg
    69721 cagagtcggg ttgaatgcgg cgttttcagc gtagctgcca ctgaaaatac gaccgtcttt
    69781 acattccagc gcgacaccgc ttggcgactt actgtaaggc atgtgcgaac ggtttgccgc
    69841 tgcaatcgcc gcctgagaaa gcgcatcacc agtcagcgca tagccgtgat cctgctcgtc
    69901 catcagcagc gttttaatct ccagatcttt cggcccaaag gcatccggca gatagtcacg
    69961 cagcgcgtgt gcctcgcggc ccggcagatg aatacgcaga tccagaccgc tgttcagttc
    70021 attcataaac tgacggcagt gaccacaagg cgtgtagtta acggtgatgg ctgcaagcgc
    70081 tttttcacca ctcaaccagg cgtggctgat cgcgctttgt tcggcatgaa cggtttgctg
    70141 cattgtcgca ccaataaatt ccatattggc accgaaatac caggttccgc tcacaccgcg
    70201 cgcaattgcg ccaacattaa aattcgacaa tggcgtacgc gcacaggccg ccgccagcgg
    70261 aagtagtgcg aatgccagcg cgtcttcgtc cagccccgtt gcgctcttca gcgatgagac
    70321 ttgctccccg gtcaacaaag cggggaagta cttgtctgcc agaataggtt ccagtgcaga
    70381 ttgcaagtta tccgcaagtt gggcaaaagc ggtttgaaaa cgtggatgca tggcgttgcc
    70441 tcataatcat gtaatgggat aacagtttac gaacccgtta tggctttata tgtgatctga
    70501 atctcattaa ttatgcaacg cgtgaaactt acatcaaaaa tgcgcgacgc atcgcaaatt
    70561 ttagcccatt actgccagaa taatcgggaa aaggaacggt gcgatcagcg aagtaattat
    70621 cccgcataac accagcgcta gcgaactaaa tgcaccttcc tgataatcca gctcggcgca
    70681 gcgcgccgtg ccgagggcgt gcgaggcagt ccccattgcc agaccgcgcg cagctttggt
    70741 acgaatacgc atcgcattaa gcaatgtatg gccaaatacc gcgccgagga tgccgacgaa
    70801 aatcacgcaa actgcgctga ttgccggaat accgccaata ctgccgccaa ccgccattgc
    70861 aataggcgtg gtgactgatt tcggcaggat tgacgcggcg atttccggtg aagcgcccat
    70921 caacaatgcc acggaagtcc ccgtcaccat cgcaaccacg ctgccgataa aacagatggt
    70981 gatgatcgat ttccagcgcg cgcggatctg gtgtagctgc tcatataaag gataggccag
    71041 cgcgacgacc gccggttgca gcagatcgtt tagcacttcg ctacctttaa agtagctgtc
    71101 gtaagagatg ccggtcagca ttaaaaaagg gatgatcacc accattgcta ccagcaacgg
    71161 gttaagcaag ggaaacttat accgtgccgc cagtttgcgg gcggcaaaaa agacaatcaa
    71221 tgtcagtggt aatgaccacc agatattcgc catcattctt ctgatccttt ctgacctacg
    71281 actttacgtt caccgtgcac cagttgcgaa ctccagctca ccaccagaaa aaccaccagc
    71341 gtactgacag cacaggaaac cactaccggg ccaaactgtg cgcggagcaa atcaaaatat
    71401 tgcatgacgc ctacgccaat cggcacaaac aatagcgcca tatagcgaat cagtacgtag
    71461 caccccggat tgacccattt tgccggaaga atttgcaagg ccagcaggac aaacaggatc
    71521 agcatcccga tgatgctgcc cggaatggtt accggtagca gggaagcaat gaaaatgcct
    71581 gcatacaggc aggcataaat caggacgaaa gcgcgtaaat attgccagat aatgttcagt
    71641 gtcttactca taatcaacac cccagatgag atggattcat catacaatta attcgtaaaa
    71701 cgtgctaccg atcacattaa gaaatcagcg cttatttttt cgctccccgg cattacgcaa
    71761 tgcgcaacga actcaggcat acatcaagca tagagaggga ttacagtttg cagcaatccc
    71821 tctttgcaga cctttaatcc gttgaccata ggcgctaaca tagcgcctca tttttttgcg
    71881 ggtgaagata tgcgtgtgtt actggctccg atggagggtg tgcttgactc tctggtgcgt
    71941 gaattgctga ccgaagttaa cgactacgat ctgtgcatca ccgagtttgt ccgcgtggtg
    72001 gatcaactgc tgccggtaaa agtttttcat cgcatttgcc ctgaactaca aaacgccagc
    72061 cggacaccat ccggtacgct ggtgcgcgtg cagttgttgg gtcagttccc acaatggctg
    72121 gcagagaacg ccgcccgtgc ggtggaatta ggttcctggg gcgtggatct caattgcggc
    72181 tgcccgtcga aaacggtcaa cggtagcggt ggcggggcga cgttactcaa agatcctgaa
    72241 ctcatctacc agggtgcaaa agcgatgcgt gaagctgtac cggcgcattt gcccgtcagc
    72301 gtgaaagtgc gtctgggctg ggacagcggt gagaagaaat ttgaaatcgc cgatgcggtt
    72361 caacaggctg gtgctacgga gctggtggtg catggacgga caaaagagca gggttaccgc
    72421 gcggagcata ttgactggca ggcgattggc gagattcgcc agcggctgaa tattccggtg
    72481 attgccaacg gtgaaatctg ggactggcag agtgcgcaac aatgcatggc gatcagcggc
    72541 tgcgacgcag tgatgattgg tcgcggggcg ctcaatattc ccaacctgag ccgggtggta
    72601 aaatataacg aaccgcgaat gccgtggccg gaggtggttg ctttgctgca aaaatatacc
    72661 tgtctggaaa agcagggcga taccgggtta tatcacgttg cgcggattaa acagtggttg
    72721 agttatttgc gtaaagaata cgatgaagca acggaattat ttcagcatgt tcgggtgttg
    72781 aataattccc ctgatattgc aagggctatt caggcaattg atatcgataa gctgcgataa
    72841 tagtggaatc gcagtttatc agagacaatg aatcgcttat tcagcttcat catcttgttg
    72901 cgctcctttc atgagctaag ccacatattg ccactggcgc aaggagcgcg cagggggagg
    72961 acaatcgcct cctccccctg cacccccggg ctctggcgaa caaaatcgcc gctgcgcggt
    73021 accttcagct tatccccttc ggctaccggg tcgggcgcga ggtaacgtcc ttgtaagacg
    73081 cgccctcagc ccacatccat gtgggctgcc ccggccttcg gggaacgctt cagcgatttt
    73141 gacgccacca acaccggagc tgtttgcaaa tcaagtacac aaaatatcta gttcctgacc
    73201 tgacaactta tatgccatat ctgattttta aaatatcatc ttaaatacgc cagtcaccac
    73261 cagtagccca atcaggaaaa taatcaatac agcccagagt ataattttca ttggcgttcc
    73321 tttatttgtg agtgctgaat aagtatagga aaaaacgaag ccctttgccg ttaaccataa
    73381 acgattaaga taaatcggaa gatatcattt acttagtcta tatttttctg ggtatgtcgt
    73441 tttattgcta ctgatgtatt gccacgcgaa actaaaaaat atatcgcaac acacggtaat
    73501 ctccgcgcac cgtttcttac ctgattattc ccccatgaat cgtgattcct tttatccagc
    73561 catcgcctgt tttccgctgt tactgatgct ggccgggtgt gcgcctatgc atgaaacccg
    73621 ccaggcgtta agccagcaaa cgcccgctgc acaagttgac accgcattac ccacggcgct
    73681 gaaaaatggt tggccagaca gccaatggtg gctggagtat cacgataatc aactcacttc
    73741 attaattaac aatgcactac agaacgcacc ggatatgcag gtggctgaac agcgcattca
    73801 gctagctgaa gcccaggcga aggcagttgc cactcaggat ggtccgcaga tcgacttttc
    73861 ggcggatatg gagcggcaaa aaatgtcggc agaaggctta atggggccgt ttgctctgaa
    73921 cgatccggcc gcaggtacga ccggcccgtg gtacaccaac ggtacttttg gcttaacggc
    73981 gggctggcat ctcgatatct ggggaaagaa tcgggcggag gttactgccc gcctgggtac
    74041 ggttaaagca cgggcggcgg aacgcgagca aacccgccaa ttgctggctg gcagcgtagc
    74101 ccgcctgtac tgggagtggc aaacccaggc ggcgttaaac acggtcttgc agcaaataga
    74161 aaaagagcag aacaccatta tcgcgaccga tcgccagcta tatcagaacg ggattacttc
    74221 ttcagttgaa ggtgtggaaa ccgatattaa tgccagcaaa acccggcagc agctcaacga
    74281 tgtcgcgggg aaaatgaaaa ttattgaggc acggttaagc gcacttacaa ataaccagac
    74341 aaagtcattg aagcttaaac cggtcgcgtt gccgaaagtg gcaagccagc ttcctgatga
    74401 actggggtac tccttactgg cccggcgggc agatttgcag gcggcgcact ggtacgttga
    74461 gtcatcgcta agcaccattg atgcggcaaa agcggcattt tatcctgaca tcaacctgat
    74521 ggccttcctg caacaggatg cgttgcactt aagcgatctg ttccgtcatt ccgcgcagca
    74581 aatgggcgtt acggcaggcc tgacgctacc cattttcgat agtggtcgtc ttaacgccaa
    74641 tctcgatatc gcaaaagccg aaagcaactt gtctatcgcc agctacaaca aagcggtggt
    74701 tgaagcggtg aatgacgtgg cgcgggcagc cagtcaggtt cagacactgg cggagaaaaa
    74761 ccagcatcag gcgcaaattg agcgcgatgc cttgcgtgtg gtaggtcttg cgcaggcccg
    74821 ctttaacgcg ggcatcattg ctggttcccg cgtcagcgaa gccagaatcc ccgcgctgcg
    74881 tgagcgggcc aatggcctgt tattgcaagg gcagtggctg gatgcctcca ttcaactcac
    74941 tggtgcgttg ggcgggggtt acaaacgctg atgatatgcg ccttctatac ttaacgttta
    75001 ttcagcgtta agtggagaac tcgatggcac aggttgcgat tattaccgcc tccgattcgg
    75061 ggatcggcaa agagtgcgcg ttattactgg cgcagcaagg gtttgatatt ggcattacct
    75121 ggcactcaga tgaagaaggg gcaaaagata ccgcgcgtga ggtagttagc cacggcgtac
    75181 gtgcggagat cgtgcagctg gatctcggta aactgccaga aggtgcgcag gcgctggaga
    75241 aactcattca acggctgggg cgtattgatg tgctggtgaa taatgcgggc gcaatgacca
    75301 aagcgccgtt tcttgatatg gctttcgatg agtggcgcaa gatttttacc gttgatgtcg
    75361 atggtgcatt tttatgctca caaattgcgg cccgccagat ggtgaaacaa gggcagggcg
    75421 gtcgcatcat caacattacg tcggtacatg aacatacgcc gctgccggat gccagcgcct
    75481 acacagccgc taaacatgcg ctcggtgggt taaccaaagc gatggcgctg gagctggtca
    75541 ggcataagat tttggtgaac gcagtcgcgc ctggggcgat cgccacgcca atgaatggca
    75601 tggatgacag cgacgtgaag cccgacgcgg agccttcgat tcctctgcgg cgttttggcg
    75661 caacgcatga gattgccagc ctggtagcgt ggctgtgctc agagggcgca aattacacca
    75721 ccgggcagtc gttgatagtg gatggcggct ttatgttggc gaatccgcag ttcaacccag
    75781 aatagcgcag tgaggtgcgt ggttatttca atgggtaaaa ttttggtagg catgataaga
    75841 cgcacaagcg tcgcatcagg catggtgcac cggatgtcgg atgcggcgcg aacgccttac
    75901 ccagggcaat ggttttacgc ctgatgatcc ggctttttct tcccgcgccg tttcagccac
    75961 cagcgcacgc ccaccaccag aaccacgacc agaatcaacc agacccagtg cttcaaatgc
    76021 tggtcgagat tgtgcaacca cggcgcaatc acctgaccac cagcgtaacc aatagtggta
    76081 aaaatcaacg cccaggcaaa tgcgccgaga atattcagcg gcagaaagat tttcggcggc
    76141 agctggctgg caccaatcag cgtcgggcca atcacccgaa agccatacat aaagcgcgta
    76201 ccaatgacaa acagatacgg atggcgttgg ataagtttct gcgcccgctc aattttatcc
    76261 tgatgtttcg agaaacggcg taacagcttg ccgccaaacc gccgcccgca cagataaagc
    76321 acctggtcgc caatcatgcc gccaagcgcc acagaaagta ccaccagcgg gaactttaat
    76381 agcccctgat gtgccgcaac gcctcccagc aaagtcacgg tttcaccttc cgccaggcta
    76441 ccgatcacca gcgcggcata accatattgt gagatgagtg tattgagatc cataggccag
    76501 tctgtctccc tttggttgcc tgtaagcata caccctggaa atcattcacg ctgaaaaagg
    76561 acgtacatca gacggtgcaa attacatgag cgaacaaaaa ataatcacaa gctgacttat
    76621 acttgaactg tcaggttcat gaactcacaa caagggggcg gtatgagcca tgtctgggga
    76681 ctgttttccc atcccgatcg tgaaatgcag gtgattaatc gcgaaaacga aacgatttct
    76741 caccattaca cccaccacgt tttgctgatg gcggcgattc cggtgatttg cgccttcatt
    76801 ggcactacac agattggctg gaattttggc gatggcacta tcctgaagtt atcctggttt
    76861 accggactgg cgctggctgt cctgttttat ggcgttatgc tggctggggt tgcggtaatg
    76921 gggcgggtca tctggtggat ggcgcgtaat tatccgcagc gtccgtcact tgcgcactgc
    76981 atggtcttcg cgggctacgt tgcaactccg ctgtttttaa gcggtctggt ggcgctttat
    77041 ccactggtct ggttgtgtgt gctggtcggc acggtggcgc tgttttacac cggctacctg
    77101 ctttaccttg gtattccgag cttcctgaat atcaacaaag aggaaggcct tagcttctct
    77161 agttcgacgc tggcgattgg cgtgctggta ctggaagtgc tgctcgccct aaccgttata
    77221 ctatggggct acggttatcg tttgttctag aaagttcttt gacgttgcat tgctggcgta
    77281 aatgtcagca atgcagcatt tcctcacgat tctcctttga cgatcgcact ttttgctcgt
    77341 tatgatggcg tcgctagcct cagtaaatcc ttactccggc ggtgcgcaac ctgtgcgcgt
    77401 gaaccactat ctgaatgctc atcatgccga aatttcgagt ttctttattt agcctggccc
    77461 tgatgctggc tgtgcctttg gcaccgcagg ccgttgcgaa aacggcagcc gctaccaccg
    77521 cttcacaacc tgaaattgcc tccggtagcg cgatgattgt tgatctgaat accaacaaag
    77581 tgatctactc gaaccacccg gatctggtgc gtccgattgc gtctatcagc aaattaatga
    77641 ccgcgatggt tgtgctggat gcacgactgc cgctggatga aaaactaaaa gtggatatca
    77701 gccagacgcc ggagatgaaa ggggtctatt cgcgcgtacg actgaatagc gaaatcagcc
    77761 gtaaagatat gctgttgctg gcgctgatgt cttcagaaaa ccgcgcggcg gcaagccttg
    77821 cacaccatta tcccggtggt tacaaagcct ttattaaggc aatgaatgcg aaagcgaaat
    77881 cgctcggaat gaacaacacg cgctttgttg aacctaccgg attgtcggtg cataacgttt
    77941 caactgcccg tgacttaacc aaactgctca ttgccagcaa acaatatccg ttgatcgggc
    78001 agttaagtac tacccgggaa gatatggcaa ccttctctaa cccgacgtat acgctgccgt
    78061 tccgcaatac taatcatctg gtgtatcgcg ataactggaa tattcagtta accaaaaccg
    78121 gctttaccaa tgcggcgggc cattgtctgg tgatgcgtac ggttatcaat aataaaccgg
    78181 tggcgctggt agtgatggat gcgtttggca aatataccca ttttgccgat tccagccgcc
    78241 tgcgtacctg gattgaaacc ggtaaagtga tgcctgttcc agcagcagcg ttgagctata
    78301 aaaaacaaaa agccgcccaa atggcggcgg cggggcagac ggcacagaac gattaagtga
    78361 atgacggatg gcgatactct gccatccgta atttttactc cacttcctgc cagtttttcc
    78421 gtttactggt tttaccgatc cccggattca tgctgttggt cggatcgttc tcgcgataga
    78481 acttctgcaa cgtctccggt gctttataca aatgaccgac gttatgctcg gcagggtact
    78541 gcgcgccgcg ctgctgtagc agttccagca tctgttcttt taacgcatgc acatccacgc
    78601 cttttttcac tatgtaatcc tgatggaaga catagcacat aaaatggccg taatagagct
    78661 tgtgcaccag ctggctgtcg atctccggcg gtaaatgctc ataccactcg gtgtcgttac
    78721 gccgcagagc gatatccaac gccagaatgt cttcgacttc atcggaatgc accgcctgat
    78781 aacgaattgc tgcgcccgca gcggcgaaac ggtgtaaaaa cgctttgctg ccttcctccg
    78841 gcgtacagac aaagaaatcg ccttcggcct gtttgaaata atctaccagc cacgatttgg
    78901 cttcaccgac gccatcgccc gccattttta acagcagatg atgctcgtat ttatcgcgcc
    78961 agtttttcat gcgcggcggt aaatggctgg ggaacaggtg accgaatttt tgcatcgcac
    79021 ggtcagtaaa atgcggacgg aagaatttca ctttctccag catcgcatcg gtgcgtccct
    79081 tgagattaaa gaagaacggc atcttgtcgg tgcctaactt atcaatcatc aggaaggtgt
    79141 ctttgccgta tttttccgca atatcgtaga tatcccggtg catatattcc ccggcaaccg
    79201 gcagattttc gaagttagcc agaatatgac ggcggatttc ggtcagcact tccggctggt
    79261 tggtgccgat ataaaacacc tgctgatttt tttccgcttc gaaggtatct agacgtactg
    79321 caaagaccgc cagcttcccg gcgcaaccag aagattcaaa taaccgatca ggatcggcgt
    79381 tataacgtgc gggcgtgtcg gcttcaatat cacgaacgcg gtggacatag tcataatcgt
    79441 gggcgtgacg accatcgtga cgcacatcgt catctttgat gcgatcatca tccagcttgc
    79501 taaggatctg ctccggcgtt tcgcccagat caatccccag atggttcacc agcgtcagtt
    79561 tgccgtcttc atttatacgc gcgaataacg acatttcggt atacgccggg ccacgttgca
    79621 ccagcgagcc gccggagttg ttacaaatac cgccgatgac cgatgcgcct atacacgatg
    79681 atccaatcac tgagtgcggt tcgcgtccca gcggtttgag ggctttttcc agcgaataga
    79741 gcgtggtgcc cggataggcc agcacctgtt cgcccttgcc aagaacgtgc agcttgtcga
    79801 gacgcagggt gctgatgatc acgatatcgc gatcatagtc gttaccgttt ggcgtcgatc
    79861 cttcggtcag gcctgtattg gcagcctgca tcagaataat tttgtcggcg gtgacgcagg
    79921 ctttcagcac ccgccacaat tccagtagtg agccagggaa aacgacagcc agcgcgtcgc
    79981 cctgaccaga acggaagccc ttgcgatagc gggccgtttt tgcgggatcg gtgagcaggt
    80041 gtgaatgacc gaccagacgg gcaagttcat tcaaaaaggc tttattatca gttgttgtca
    80101 tggaagacat tttccactcc ttgtggtggc gaaaaaatat cacaaactag aatagcgcct
    80161 tgtatgacaa ttcagcgagt gaatattttg aaaattcaga caataaactg agcattcttt
    80221 ttctctctcc atcatgctta tggcaaacta cggtttttgc accattttcg ctgcctcgtc
    80281 gggcaacaga ggaagaaaaa tccatatgaa atggctatgt tcagtaggaa tcgcggtgag
    80341 tctggccctg cagccagcac tggcggatga tttattcggc aaccatccat taacgcccga
    80401 agcgcgggat gcgttcgtca ccgaactgct taagaaaatg acagttgatg agaaaattgg
    80461 tcagctgcgc ttaatcagcg tcggcccgga taacccgaaa gaggcgatcc gcgagatgat
    80521 caaagacggt caggttgggg cgattttcaa caccgtaacc cgtcaggata tccgcgccat
    80581 gcaggatcag gtgatggaat taagccgcct gaaaattcct cttttctttg cttacgacgt
    80641 gctgcacggt cagcgcacgg tgttcccgat tagcctcggt ctggcctcgt cttttaacct
    80701 cgatgcagtg aaaacggtcg gacgtgtctc tgcttatgaa gcggcagatg atggcctgaa
    80761 tatgacctgg gcaccgatgg tcgatgtctc gcgcgatccg cgctggggac gtgcttccga
//
LOCUS       DAJOSY010000005        79899 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJOSY010000005 DAJOSY010000000
VERSION     DAJOSY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.005"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            5..589
                     /locus_tag="OHN27_000355"
     CDS             5..589
                     /locus_tag="OHN27_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2284864.1"
                     /translation="MNKGTKIAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPMTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGIAATGVGIRIDDAESGNIMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            669..1364
                     /locus_tag="OHN27_000356"
     CDS             669..1364
                     /locus_tag="OHN27_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCQ2284865.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKIDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1394..3910
                     /locus_tag="OHN27_000357"
     CDS             1394..3910
                     /locus_tag="OHN27_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2284866.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGVGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3921..5012
                     /locus_tag="OHN27_000358"
     CDS             3921..5012
                     /locus_tag="OHN27_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2284867.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKNNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5055..5915)
                     /gene="rsmI"
                     /locus_tag="OHN27_000359"
     CDS             complement(5055..5915)
                     /gene="rsmI"
                     /locus_tag="OHN27_000359"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCQ2284868.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5980..8016
                     /gene="lpoA"
                     /locus_tag="OHN27_000360"
     CDS             5980..8016
                     /gene="lpoA"
                     /locus_tag="OHN27_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCQ2284869.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDAQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITLRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRNLSWLQYQQGQVVPVS"
     gene            7974..8369
                     /locus_tag="OHN27_000361"
     CDS             7974..8369
                     /locus_tag="OHN27_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCQ2284870.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTIFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8389..8979
                     /gene="diaA"
                     /locus_tag="OHN27_000362"
     CDS             8389..8979
                     /gene="diaA"
                     /locus_tag="OHN27_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCQ2284871.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8989..9564
                     /gene="yraP"
                     /locus_tag="OHN27_000363"
     CDS             8989..9564
                     /gene="yraP"
                     /locus_tag="OHN27_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCQ2284872.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9678..10718)
                     /locus_tag="OHN27_000364"
     CDS             complement(9678..10718)
                     /locus_tag="OHN27_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCQ2284873.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10791..11426)
                     /locus_tag="OHN27_000365"
     CDS             complement(10791..11426)
                     /locus_tag="OHN27_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCQ2284874.1"
                     /translation="MSQVLITGSTGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWQKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11554..12072
                     /gene="yhbO"
                     /locus_tag="OHN27_000366"
     CDS             11554..12072
                     /gene="yhbO"
                     /locus_tag="OHN27_000366"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCQ2284875.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12052..12495)
                     /locus_tag="OHN27_000367"
     CDS             complement(12052..12495)
                     /locus_tag="OHN27_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCQ2284876.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12546..12848
                     /gene="yhbQ"
                     /locus_tag="OHN27_000368"
     CDS             12546..12848
                     /gene="yhbQ"
                     /locus_tag="OHN27_000368"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCQ2284877.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12835..13338)
                     /locus_tag="OHN27_000369"
     CDS             complement(12835..13338)
                     /locus_tag="OHN27_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2284878.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13332..13856)
                     /locus_tag="OHN27_000370"
     CDS             complement(13332..13856)
                     /locus_tag="OHN27_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCQ2284879.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14065..15060
                     /locus_tag="OHN27_000371"
     CDS             14065..15060
                     /locus_tag="OHN27_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCQ2284880.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15069..15947
                     /locus_tag="OHN27_000372"
     CDS             15069..15947
                     /locus_tag="OHN27_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCQ2284881.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16028..17035
                     /locus_tag="OHN27_000373"
     CDS             16028..17035
                     /locus_tag="OHN27_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCQ2284882.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17153..18397)
                     /gene="mtr"
                     /locus_tag="OHN27_000374"
     CDS             complement(17153..18397)
                     /gene="mtr"
                     /locus_tag="OHN27_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCQ2284883.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18551..20440)
                     /gene="deaD"
                     /locus_tag="OHN27_000375"
     CDS             complement(18551..20440)
                     /gene="deaD"
                     /locus_tag="OHN27_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCQ2284884.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20433..20513)
                     /gene="yrbN"
                     /locus_tag="OHN27_000376"
     CDS             complement(20433..20513)
                     /gene="yrbN"
                     /locus_tag="OHN27_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCQ2284885.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20620..21504)
                     /gene="nlpI"
                     /locus_tag="OHN27_000377"
     CDS             complement(20620..21504)
                     /gene="nlpI"
                     /locus_tag="OHN27_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCQ2284886.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21613..23748)
                     /gene="pnp"
                     /locus_tag="OHN27_000378"
     CDS             complement(21613..23748)
                     /gene="pnp"
                     /locus_tag="OHN27_000378"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCQ2284887.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAQLLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNAHVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23995..24264)
                     /gene="rpsO"
                     /locus_tag="OHN27_000379"
     CDS             complement(23995..24264)
                     /gene="rpsO"
                     /locus_tag="OHN27_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCQ2284888.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24413..25357)
                     /gene="truB"
                     /locus_tag="OHN27_000380"
     CDS             complement(24413..25357)
                     /gene="truB"
                     /locus_tag="OHN27_000380"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCQ2284889.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVKYPA"
     gene            complement(25357..25758)
                     /gene="rbfA"
                     /locus_tag="OHN27_000381"
     CDS             complement(25357..25758)
                     /gene="rbfA"
                     /locus_tag="OHN27_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCQ2284890.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25922..28594)
                     /gene="infB"
                     /locus_tag="OHN27_000382"
     CDS             complement(25922..28594)
                     /gene="infB"
                     /locus_tag="OHN27_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCQ2284891.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28619..30106)
                     /gene="nusA"
                     /locus_tag="OHN27_000383"
     CDS             complement(28619..30106)
                     /gene="nusA"
                     /locus_tag="OHN27_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCQ2284892.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30134..30586)
                     /gene="rimP"
                     /locus_tag="OHN27_000384"
     CDS             complement(30134..30586)
                     /gene="rimP"
                     /locus_tag="OHN27_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCQ2284893.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30793..30869)
                     /locus_tag="OHN27_000385"
     tRNA            complement(30793..30869)
                     /locus_tag="OHN27_000385"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30833..30835),aa:Met,seq:cat)
     gene            31217..32560
                     /gene="argG"
                     /locus_tag="OHN27_000386"
     CDS             31217..32560
                     /gene="argG"
                     /locus_tag="OHN27_000386"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCQ2284894.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(32568..34193)
                     /locus_tag="OHN27_000387"
     CDS             complement(32568..34193)
                     /locus_tag="OHN27_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCQ2284895.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34652..34738)
                     /locus_tag="OHN27_000388"
     tRNA            complement(34652..34738)
                     /locus_tag="OHN27_000388"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34702..34704),aa:Leu,seq:gag)
     gene            complement(34753..35085)
                     /gene="secG"
                     /locus_tag="OHN27_000389"
     CDS             complement(34753..35085)
                     /gene="secG"
                     /locus_tag="OHN27_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCQ2284896.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35313..36650)
                     /gene="glmM"
                     /locus_tag="OHN27_000390"
     CDS             complement(35313..36650)
                     /gene="glmM"
                     /locus_tag="OHN27_000390"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCQ2284897.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36643..37491)
                     /gene="folP"
                     /locus_tag="OHN27_000391"
     CDS             complement(36643..37491)
                     /gene="folP"
                     /locus_tag="OHN27_000391"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCQ2284898.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37581..39515)
                     /gene="ftsH"
                     /locus_tag="OHN27_000392"
     CDS             complement(37581..39515)
                     /gene="ftsH"
                     /locus_tag="OHN27_000392"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCQ2284899.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39615..40244)
                     /gene="rlmE"
                     /locus_tag="OHN27_000393"
     CDS             complement(39615..40244)
                     /gene="rlmE"
                     /locus_tag="OHN27_000393"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCQ2284900.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40370..40663
                     /gene="yhbY"
                     /locus_tag="OHN27_000394"
     CDS             40370..40663
                     /gene="yhbY"
                     /locus_tag="OHN27_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCQ2284901.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40819..41295)
                     /gene="greA"
                     /locus_tag="OHN27_000395"
     CDS             complement(40819..41295)
                     /gene="greA"
                     /locus_tag="OHN27_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCQ2284902.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41543..42976
                     /gene="dacB"
                     /locus_tag="OHN27_000396"
     CDS             41543..42976
                     /gene="dacB"
                     /locus_tag="OHN27_000396"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCQ2284903.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43162..44334)
                     /gene="cgtA"
                     /locus_tag="OHN27_000397"
     CDS             complement(43162..44334)
                     /gene="cgtA"
                     /locus_tag="OHN27_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCQ2284904.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44350..45315)
                     /locus_tag="OHN27_000398"
     CDS             complement(44350..45315)
                     /locus_tag="OHN27_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2284905.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45442..45699)
                     /gene="rpmA"
                     /locus_tag="OHN27_000399"
     CDS             complement(45442..45699)
                     /gene="rpmA"
                     /locus_tag="OHN27_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCQ2284906.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45720..46031)
                     /gene="rplU"
                     /locus_tag="OHN27_000400"
     CDS             complement(45720..46031)
                     /gene="rplU"
                     /locus_tag="OHN27_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCQ2284907.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46290..47261
                     /gene="ispB"
                     /locus_tag="OHN27_000401"
     CDS             46290..47261
                     /gene="ispB"
                     /locus_tag="OHN27_000401"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCQ2284908.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47490..47768
                     /gene="sfsB"
                     /locus_tag="OHN27_000402"
     CDS             47490..47768
                     /gene="sfsB"
                     /locus_tag="OHN27_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCQ2284909.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47816..49075)
                     /gene="murA"
                     /locus_tag="OHN27_000403"
     CDS             complement(47816..49075)
                     /gene="murA"
                     /locus_tag="OHN27_000403"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCQ2284910.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49130..49384)
                     /gene="ibaG"
                     /locus_tag="OHN27_000404"
     CDS             complement(49130..49384)
                     /gene="ibaG"
                     /locus_tag="OHN27_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCQ2284911.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49544..49837)
                     /gene="mlaB"
                     /locus_tag="OHN27_000405"
     CDS             complement(49544..49837)
                     /gene="mlaB"
                     /locus_tag="OHN27_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCQ2284912.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49837..50472)
                     /gene="mlaC"
                     /locus_tag="OHN27_000406"
     CDS             complement(49837..50472)
                     /gene="mlaC"
                     /locus_tag="OHN27_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCQ2284913.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50491..51042)
                     /gene="mlaD"
                     /locus_tag="OHN27_000407"
     CDS             complement(50491..51042)
                     /gene="mlaD"
                     /locus_tag="OHN27_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCQ2284914.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(51047..51829)
                     /gene="mlaE"
                     /locus_tag="OHN27_000408"
     CDS             complement(51047..51829)
                     /gene="mlaE"
                     /locus_tag="OHN27_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCQ2284915.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51837..52646)
                     /gene="mlaF"
                     /locus_tag="OHN27_000409"
     CDS             complement(51837..52646)
                     /gene="mlaF"
                     /locus_tag="OHN27_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCQ2284916.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52856..53833
                     /locus_tag="OHN27_000410"
     CDS             52856..53833
                     /locus_tag="OHN27_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCQ2284917.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53847..54833
                     /gene="kdsD"
                     /locus_tag="OHN27_000411"
     CDS             53847..54833
                     /gene="kdsD"
                     /locus_tag="OHN27_000411"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCQ2284918.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54854..55420
                     /gene="kdsC"
                     /locus_tag="OHN27_000412"
     CDS             54854..55420
                     /gene="kdsC"
                     /locus_tag="OHN27_000412"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCQ2284919.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55417..55992
                     /gene="lptC"
                     /locus_tag="OHN27_000413"
     CDS             55417..55992
                     /gene="lptC"
                     /locus_tag="OHN27_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCQ2284920.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55961..56518
                     /gene="lptA"
                     /locus_tag="OHN27_000414"
     CDS             55961..56518
                     /gene="lptA"
                     /locus_tag="OHN27_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCQ2284921.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56525..57250
                     /gene="lptB"
                     /locus_tag="OHN27_000415"
     CDS             56525..57250
                     /gene="lptB"
                     /locus_tag="OHN27_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCQ2284922.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57298..58731
                     /gene="rpoN"
                     /locus_tag="OHN27_000416"
     CDS             57298..58731
                     /gene="rpoN"
                     /locus_tag="OHN27_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCQ2284923.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58754..59041
                     /gene="hpf"
                     /locus_tag="OHN27_000417"
     CDS             58754..59041
                     /gene="hpf"
                     /locus_tag="OHN27_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCQ2284924.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59159..59650
                     /gene="ptsN"
                     /locus_tag="OHN27_000418"
     CDS             59159..59650
                     /gene="ptsN"
                     /locus_tag="OHN27_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCQ2284925.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59696..60550
                     /gene="rapZ"
                     /locus_tag="OHN27_000419"
     CDS             59696..60550
                     /gene="rapZ"
                     /locus_tag="OHN27_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCQ2284926.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60547..60819
                     /gene="npr"
                     /locus_tag="OHN27_000420"
     CDS             60547..60819
                     /gene="npr"
                     /locus_tag="OHN27_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCQ2284927.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            61032..61664
                     /gene="yrbL"
                     /locus_tag="OHN27_000421"
     CDS             61032..61664
                     /gene="yrbL"
                     /locus_tag="OHN27_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCQ2284928.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61661..62389)
                     /gene="mtgA"
                     /locus_tag="OHN27_000422"
     CDS             complement(61661..62389)
                     /gene="mtgA"
                     /locus_tag="OHN27_000422"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCQ2284929.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPRRFKVSAPSGYVHS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62386..63039)
                     /gene="elbB"
                     /locus_tag="OHN27_000423"
     CDS             complement(62386..63039)
                     /gene="elbB"
                     /locus_tag="OHN27_000423"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCQ2284930.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63269..65605)
                     /gene="arcB"
                     /locus_tag="OHN27_000424"
     CDS             complement(63269..65605)
                     /gene="arcB"
                     /locus_tag="OHN27_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCQ2284931.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65701..66630)
                     /locus_tag="OHN27_000425"
     CDS             complement(65701..66630)
                     /locus_tag="OHN27_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCQ2284932.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLTRQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            67305..71765
                     /gene="gltB"
                     /locus_tag="OHN27_000426"
     CDS             67305..71765
                     /gene="gltB"
                     /locus_tag="OHN27_000426"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCQ2284933.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEQMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNTELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71778..73196
                     /gene="gltD"
                     /locus_tag="OHN27_000427"
     CDS             71778..73196
                     /gene="gltD"
                     /locus_tag="OHN27_000427"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCQ2284934.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73380..73859
                     /locus_tag="OHN27_000428"
     CDS             73380..73859
                     /locus_tag="OHN27_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCQ2284935.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            73876..74181
                     /locus_tag="OHN27_000429"
     CDS             73876..74181
                     /locus_tag="OHN27_000429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2284936.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(74257..74721)
                     /locus_tag="OHN27_000430"
     CDS             complement(74257..74721)
                     /locus_tag="OHN27_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCQ2284937.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74718..75593)
                     /gene="nanK"
                     /locus_tag="OHN27_000431"
     CDS             complement(74718..75593)
                     /gene="nanK"
                     /locus_tag="OHN27_000431"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCQ2284938.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75590..76279)
                     /locus_tag="OHN27_000432"
     CDS             complement(75590..76279)
                     /locus_tag="OHN27_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCQ2284939.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALVQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76327..77817)
                     /gene="nanT"
                     /locus_tag="OHN27_000433"
     CDS             complement(76327..77817)
                     /gene="nanT"
                     /locus_tag="OHN27_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCQ2284940.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(77927..78820)
                     /gene="nanA"
                     /locus_tag="OHN27_000434"
     CDS             complement(77927..78820)
                     /gene="nanA"
                     /locus_tag="OHN27_000434"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCQ2284941.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(78942..79733)
                     /gene="nanR"
                     /locus_tag="OHN27_000435"
     CDS             complement(78942..79733)
                     /gene="nanR"
                     /locus_tag="OHN27_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCQ2284942.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        19692 a        21572 c        19834 g        18801 t
ORIGIN      
        1 atatatgaat aaaggtacaa aaatagccat tgcgggctta cttgcgcttt tcgcaggtaa
       61 tgccgctgca acagatggtg aaatagtttt tgatggcgag attttaaaat ccgcttgtga
      121 aatcaatgac tctgataaga aaattgaagt tgctcttggt cactataatg ccgaacagtt
      181 tcgttctgtt ggcgatcgta gcccaaaaat tccattcact attcctttag ttaactgccc
      241 aatgactggg tgggagcacg ataacggcaa cgttgaagcg tctttccgtc tatggctaga
      301 aacgcgggat aatggtaccg ttcctaactt ccctaatctg gcaaaagtag gctcttttgc
      361 tggcatagcg gcaacaggtg tgggtatccg tattgacgat gcggaaagcg gaaacattat
      421 gccactgaat gctatgggca atgataacac ggtttatcag atcccggcgg actccaatgg
      481 tattgtcaat gttgacctca tcgcttacta cgtatcaacc gttgaagcat cagaaatcac
      541 cccaggggaa gcagacgctg tcgttaacgt aacgctggat taccgttaat acgttacggc
      601 gttatctgac ctgtcagata acgccctttt ccttcctctt tctcgttgta tcaggttgaa
      661 aaataactat gtcaaaacga acattcgcgg tgatattaac cttgttgtgt agcttctgta
      721 ttggccaggc gcttgcagga ggaatcgttt tacagcgaac gcgagtgatc tatgatgcca
      781 gccgcaaaga ggctgcgtta cctgtcgcaa acaaaggcgc agaaacgcct tatttactgc
      841 aatcatgggt agataatata gatggtaaaa gccgtgcccc atttattata accccaccgc
      901 tatttcgtct tgaggctggc gatgactcat cactgcgaat tattaaaaca gctgataacc
      961 tgcctgaaaa taaagagtcg ctgttctaca ttaatgttcg tgccattcca gcaaagaaaa
     1021 aatcagatga tgttaatgct aacgagttga cgctggtatt taaaacacgg atcaaaatgt
     1081 tttatcgccc cgcacacctg aagggacggg taaacgatgc gtggaaatca ctggaattta
     1141 aacgtagtga tcattcactc aatatatata acccaactga atattacgtc gtatttgccg
     1201 gattggcagt cgataaaatc gatctcacaa gcaaaattga atatatcgcg cccggagaac
     1261 ataaacagtt accacttcct gcatctggcg gaaagaacgt gaaatgggct gcgatcaatg
     1321 attatggcgg cagttccggg acagaaactc gtccactgca ataacaaata taaaaaacac
     1381 aggtcatcag ggaatgccac aacgacacca ccagggacat aaacgcacac cgaaacagtt
     1441 ggcgctcatt atcaaacgct gtttgccgat ggtgctcact ggcagcggca tgctttgcac
     1501 taccgctaac gccgaagagt attatttcga ccccattatg ctggaaacca caaaaagtgg
     1561 tatgcaaaca accgatctgt cacgattttc aaagaaatac gcacaactac caggaactta
     1621 tcaggttgat atctggctga ataaaaagaa ggtttcacag aaaaaaatta catttaccgc
     1681 caatgcagag caacttctgc agccacagtt tacggtagaa caactacgtg agctgggtat
     1741 taaggtggat gaaatcccgg cgctggctga aaaagatgac gatagcgtga tcaactcgct
     1801 tgaacaaatc attcccggta cagctgctga atttgatttc aatcatcagc gacttaattt
     1861 gagcattccc caaattgcac tgtaccgtga tgcaagaggt tacgtctccc cttctcgttg
     1921 ggacgatggt ataccaacgc tgtttaccaa ctactcgttt acaggttctg ataaccgtta
     1981 ccgccagggc aatcgtagcc aacgacagta cctgaatatg caaaatggtg ctaattttgg
     2041 cccctggcga ttacgcaact attccacatg gacacgcaac gatcagacat caagctggaa
     2101 taccatcagt agttatttac aacgtgatat caaggcattg aagtctcagt tgcttctggg
     2161 agaaagcgcc accagcggca gtattttttc cagctacacc tttactggcg ttcaactcgc
     2221 ttccgacgat aatatgctgc caaacagcca gcgcggattt gccccaacgg tacgcggtat
     2281 cgcaaacagt agtgcaatcg tgactatcag gcaaaatggt tatgtgatct atcaaagcaa
     2341 cgtgccagcg ggtgcctttg aaattaacga tctctacccc tcttccaaca gcggcgattt
     2401 agaagtcacg attgaagaaa gtgacggtac gcaacgtcgc tttatccagc cttattcttc
     2461 attacccatg atgcagcgac ctgggcatct aaagtatagc gcgaccgctg gacgctatcg
     2521 cgctgatgca aacagtgata gcaaggaacc cgaatttgct gaagccacgg caatatatgg
     2581 tttgaataat acttttacgc tgtatggcgg cctgctcggt tctgaagatt attatgcgct
     2641 ggggatcggt atcggcggca cacttggcgc actgggcgcg ttgtcgatgg atatcaacag
     2701 agctgacacc caattcgata accagcactc ttttcatggc tatcaatggc gtacgcagta
     2761 catcaaagat atcccggaaa ccaacaccaa tatcgctgtc agctactatc gctataccaa
     2821 cgatggctat tttagtttta atgaagccaa tacccgcaat tgggactata acagtcgcca
     2881 aaaaagtgaa attcaattca acatcagcca gacaatattt gatggggtaa gtctgtatgc
     2941 ctccggttcg cagcaagact attggggcaa taacgataaa aacaggaata tctctgttgg
     3001 ggtttccggc cagcaatggg gagttggtta cagcctgaat tatcaataca gccgctacac
     3061 tgatcaaaat aatgaccgcg cactctcttt gaatctcagt attccgttag aacgctggtt
     3121 accgcgtagc cgggtttcct atcagatgac cagccagaaa gatcgcccaa cccaacatga
     3181 aatgcgtctt gatggctcac tgctggatga tggtcgcctg agctatagcc tggaacaaag
     3241 tctggatgac gataacaacc ataacagtag cctgaacgcc agttaccgtt caccttatgg
     3301 aaccttcagt gccggataca gctacggtaa tgacagcagc caatacaatt acggcgttac
     3361 cggcggcgtg gttatccatc ctcatggcgt gacgctctcg caatatctgg gcaacgcttt
     3421 tgcgcttatc gatgctaatg gggcttctgg cgtgaggata caaaactatc cggggattgc
     3481 taccgatccc tttggctatg cagtggttcc ttatctcacg acttaccagg aaaaccgtct
     3541 ctcggtagat actacgcagc tgcccgataa cgtcgatctt gagcaaacaa cacagtttgt
     3601 ggtgcccaac agaggtgcaa tggtagcggc gcgtttcaac gccaatatcg gttatcgcgt
     3661 acttgttaca gtcagcgatc gcaacggtaa accgttgccc tttggcgctc ttgccagcaa
     3721 cgatgagacg gggcaacaaa gtatcgtcga tgagggcggc atactatatc tctctgggat
     3781 atcgagtaaa tcacaaagct ggactgtacg ctggggaaat caggcagatc aacaatgtca
     3841 gtttgctttt agtacaccgg attcagaacc cacaacctct gtattacaag gcacggcgca
     3901 gtgccattaa ggataaaaaa atgaaaagag cgcctcttat aacaggactt ttgttgatat
     3961 ccacatcctg cgcttatgcc tcctcaggag ggtgtggagc cgatagcact agcggtgcga
     4021 caaattacag cagtgtggtt gatgatgtta cggtgaacca gacagataac gtgacaggac
     4081 gggagtttac ctctgcaacg ctaagtagca ctaactggca atacgcctgt tcctgctctg
     4141 cgggtaaggc agttaaactt gtctatatgg tcagccccgt acttaccacc actggacatc
     4201 agacaggata ttacaaactc aatgacagcc tggatattaa aaccacatta caggcaaacg
     4261 acattccagg actcacaacc gaccaggttg tctctgttaa cacccgattc acacagataa
     4321 aaaacaacac ggtatattct gctgcaaccc aaacgggtgt ttgccagggt gatacgtctc
     4381 gttatggacc cgttaatatt ggtgcgaaca ccacctttac cctgtatgtc accaagccat
     4441 ttctcggctc gatgaccatt ccgaaaacgg atattgccgt cattaaaggc gcgtgggtcg
     4501 atggaatggg aagcccgtct acaggtgact tccatgattt agtcaagtta tcgattcagg
     4561 gaaatctcac cgccccacag tcgtgcaaaa ttaatcaggg cgatgttatt aaggttaatt
     4621 ttggattcat caatggtcag aagtttacca cccgcaatgc catgccagac ggttttactc
     4681 cagtagactt tgatatcact tatgactgtg gtgatacttc aaagattaaa aactcgttgc
     4741 aaatgcgcat cgacggtaca actggggtag tagaccagta caacctggtc gccaggcgaa
     4801 gaagttcaga caatgtgccc gatgtcggta ttcgtattga aaatctcggc ggcggagttg
     4861 caaatattcc ttttcagaac ggtatccttc ccgttgatcc ttccgggcat ggcaccgtca
     4921 atatgcgcgc ctggccagtt aatctggtcg gtggtgagct ggaaacagga aaatttcagg
     4981 gcacagccac cattaccgtc atcgtgcggt aaaagaagac gttgcgtttt gattaacgca
     5041 acgcttcggc actgttaccc ctgctgctcc agcgcatact tatacagcgc atttttcttc
     5101 acgccgtgaa tttctgcggc cagcgccgcc gcttttttca gcggcagttc tgcctgtagc
     5161 agcgccagcg tgcgcagggc atcggcgggt aagtcttctt cctgtgcttt atgaccttcg
     5221 acaatcagca ccatttcgcc tttgcgacgg ttttcatctt cctttaccca cgccagcagc
     5281 tcgccaacgg gcgcgccgtg aatggtttcc caggttttgg tcagctcacg cgccagaacc
     5341 acgtagcggg attcgcctaa taccgcaacg atatcttcca ggctatctaa cagacggtgg
     5401 gtagattcat aaaaaatcag cgtgcgcggc tccgcttcaa tggcttttag cgcatcacgg
     5461 cggccttttg atttggcagg taaaaagcct tcgtaacaga aacggtcaga gggtaaaccc
     5521 gctgcgctta acgcagtgat agcagcacac ggcccgggta gcggcaccac gcggatcccc
     5581 gcttcacggc aggtacgcac cagatggtag ccaggatcgt taattagcgg cgttccggca
     5641 tcggaaacca gcgcaatgtt ttgcccctct tgcagcttcg ccagcagcgt ttcagctttt
     5701 tgttgttcgt tatggtcgtg cagcgcaaac aaccgggcat taatcccaaa atgttgcagc
     5761 aataaaccgg tgtgacgagt atcctcggcg gcaatcagat caacggcctg taatacctct
     5821 aacgcacgct gggtgatatc cgccagattg ccgattggcg tcggtacaat gtaaagctgg
     5881 ccctgagaat tatccgccga ttggtgttgt ttcattgtgt cgtccgtatt gccgatttaa
     5941 tattgagcat tgcgtaaaaa aaatatcact ggatacatta tggtaccctc aacattttct
     6001 cgtttgaaag ccgcgcgttg tctgcctgtt gttctggcag ccctgatttt cgccggttgt
     6061 ggcacccata ctcccgatca gtccactgct tatatgcagg gtacggcgca ggctgattct
     6121 gccttttatc ttcagcagat gcagcaaagc tctgatgata ccaggatcaa ctggcaatta
     6181 ctcgccattc gtgcactggt gaaagaaggt aaaaccgggc aggcggtgga gttgtttaac
     6241 caactaccgc aagaactgaa cgatgctcag cgccgcgaga aaacactgct ggcggtagag
     6301 attaaactgg cgcagaaaga ttttgctggc gcgcaaaact tgctggcgaa aatcacacct
     6361 gccgatttag aacaaaacca gcaagcccgt tactggcagg caaaaatcga tgccagccag
     6421 gggcgtcctt ccattgattt actgcgcgcg ttaattgctc aggaaccgct gctcggcgcg
     6481 aaagaaaagc agcagaatat tgacgccacc tggcaggcgc tctcctccat gactcaggaa
     6541 caggcgaata cgctggtgat caacgccgac gaaaatattc tgcaaggctg gctggatctg
     6601 cagcgcgtct ggtttgataa ccgtaacgat cccgacatga tgaaagccgg gatcgccgac
     6661 tggcagaaac gttatccgaa caacccgggc gcgaaaatgc tgccaacgca gttggttaac
     6721 gtaaaagcgt ttaaaccagc ctcgaccaac aaaatcgccc tgctgttgcc actgaatggc
     6781 caggcagcgg tatttggtcg cactattcag caaggctttg aagcggcgaa aaatatcggc
     6841 actcagccag tggcagctca ggtagctgcc gcacctgccg cagacgtagc tgaacaacct
     6901 cagccgcaaa ccgtggatgg cgttgccagc ccggcacaag cctcggttag cgatctgacc
     6961 ggtgaacagc ctgcagccca gccggtgcct gtaagcgccc cggcgacaag caccgcagcg
     7021 gtaagcgcac ccgcaaatcc atccgcagag ctgaaaatct acgatacctc atcacaacca
     7081 cttagccaga tcttaagcca ggttcagcag gatggcgcga gtattgtggt cggtccgttg
     7141 ctgaaaaata acgttgaaga gttgctgaag agcaacactc cgctgaacgt actggcactg
     7201 aaccagccgg agaatatcga aaatcgcgtc aatatttgtt acttcgcgct ttcaccggaa
     7261 gacgaagcgc gcgatgcagc gcgtcatatt cgtgaccagg gtaaacaagc gccgctggtg
     7321 ctgatcccac gcagttcatt gggcgatcgc gtagccaatg cgtttgcgca agagtggcag
     7381 aaactgggcg gcggcaccgt tctgcaacaa aaatttggtt ccaccagcga attacgcgcg
     7441 ggtgttaacg gcggttctgg tattgcttta acgggtagcc cgattactct cagagcgaca
     7501 accgactccg gcatgacgac caacaatcca acgctgcaaa ccacgccaac cgatgaccag
     7561 ttcaccaata atggcggtcg tgtcgatgcg gtgtacattg tggcaacgcc gggtgaaatc
     7621 gcttttatca aaccgatgat cgccatgcgt aacggtagcc agagcggtgc aacgctgtac
     7681 gccagctccc gcagtgcgca agggaccgct ggcccggatt tccgactgga gatggaaggc
     7741 ttgcagtaca gcgaaatccc gatgctggca ggcggtaatc taccgttaat gcagcaggca
     7801 ctcagcgcgg tgaataacga ttattcactg gctcgcatgt atgcgatggg cgtcgatgcc
     7861 tggtcgctgg caaatcattt ctcacaaatg cgccaggttc agggttttga aatcaacggt
     7921 aataccggaa gcctgacggc taacccggat tgcgtgatta acaggaactt atcatggcta
     7981 cagtaccaac aaggtcaggt agtccccgtc agttaaccac caaacagacc ggcgatgcgt
     8041 gggaagcaca agcgcgtcgc tggctggaag gcaaaggact gcggtttatc gccgctaacg
     8101 tgaacgagcg tggcggcgag atcgatctga taatgcgtga aggccggacc accatttttg
     8161 tcgaggtacg ctatcgccgc tctgcgcttt atggcggcgc ggcagccagt gtgacccgca
     8221 gcaaacaaca caaattatta cagactgccc gcttgtggct cgcgcgtcat aatgggagtt
     8281 ttgatactgt ggattgccgg ttcgatgtgg tagccttcac cgggaatgag gttgagtgga
     8341 ttaaggatgc ctttaatgac cactcataat taaggtttaa ggattagcgt gcaagaaaga
     8401 attaaagctt gcttcactga aagcattcaa actcaaattg cggcggcaga ggcgcttccg
     8461 gatgccatct cccgtgcagc catgacgctg gttcagtctc tgctcaatgg caacaaaatc
     8521 ctctgttgtg gtaatggaac ttccgctgcc aatgcacagc attttgctgc cagcatgatc
     8581 aaccgtttcg aaacggagcg gcccagctta cctgccattg cactaaatac tgataatgtt
     8641 gtcttaacgg cgattgccaa cgatcgctta catgatgaag tgtatgcaaa acaggtgcgg
     8701 gcgctgggtc atgcgggaga tgtattgtta gccatttcca cccgtggcaa cagccgcgat
     8761 attgttaaag cagttgaagc cgccgttacg cgtgatatga ccattgtggc attgaccggc
     8821 tatgacggcg gcgaacttgc aggtttgtta gggccacagg atgtggagat ccgcattcct
     8881 tcgcatcgta gtgctcgcat tcaggaaatg catatgctga cggtaaattg cctgtgcgat
     8941 ctgatcgata acacgctttt ccctcaccag gatgattaag gagaatacat gaaggcatta
     9001 tcgccaatcg cagtccttat ttccgcgctg ctgttgcaag gttgtgttgc cgctgccgta
     9061 gtgggtaccg ctgctgtggg taccaaagcc gcaactgacc cacgcagtgt cggcacccag
     9121 gtggacgatg gtaccctgga agtgcgcgtg aacagcgcat tgtcgaaaga cgaacagatt
     9181 aagaaagaag cgcgcattaa tgtaacggcc tatcagggca aagtgctgct ggttgggcag
     9241 tcaccaaatg ctgaactttc ggctcgcgcc aaacagattg ctatgggcgt agacggtgcc
     9301 aacgaagtgt ataacgagat tcgtcagggc cagccgattg gtctgggcga agcatctaac
     9361 gatacgtgga tcaccaccaa agtgcgttcg cagctcttaa ccagcgacct ggtgaaatcg
     9421 tccaacgtga aagtgaccac cgaaaacggt gaagtgttcc tgatggggct ggtgactgaa
     9481 cgtgaagcga aagcggcggc agatattgcc agccgggtga gcggcgtaaa gcgggtaacc
     9541 acagcgttta cgtttattaa atagcagccc cttgtaatgc ctgatgcgac gcttgccgcg
     9601 tcttatcagg cctacaacga tgaaaaccgt aggtcggata aggcgtttac gccgcatccg
     9661 acatgttttc cctcaaatca gaacaacagc gccagcccgc cgacaatcac accagacact
     9721 gccaccatcg cccctgtcag ccataaggct ttcgccggga acgctttgcg cagcataatc
     9781 agtgacggca aactcactgc cgggagcgtc atcaacaatg ccagcgccgg agcggttccc
     9841 atacctgcca gcatcatcgt ttgtacaatc ggaatttctg ctgccgtggg aatgacaaac
     9901 aagcatcctg ctaccgccat cgccaccacc cacatcaggc tgttatcgac agcaccatcg
     9961 gcatggggga ataaccagac gcgtgcggca cccaacacca gtactgcaag gatgtaaacc
    10021 gggatcgtac tccagaaaag cgtccatagc gccctgcccc agcggctaaa aaaaccgctc
    10081 tgtgcttccg gtatgtcaat ttcgaccggt gcctgcgttt gcggtgtttc acgcacccat
    10141 ttttgcacca gcgtcgcaat cagcaacacc atcaccagcc cggccaccag acgaatcgcc
    10201 gcaaaacccc agccgaggac aaaccccata aacaccagcg tcgccgggtt taacaccgga
    10261 ttgcccatcc agaatgccag cgcaccgccc atcgatacct gttggcgacg cattcccgcc
    10321 gcaaccggag ccgcacagca ggtacacatc atgcccggca gcgaaaacag cgttcccagc
    10381 agcgtgccgc gaaagcgcga ttgcccaagc gtacgcaaca accagtcacg cgggatcagc
    10441 acctgaatca acgaaccgag gatcaccccc agcaccgccg ctttccatac cgcgaggaaa
    10501 tagatcatcg cgtaatccaa cgccgcttgc cacggattag catccgcctg cgcaaggata
    10561 gatttaccga tactgtgggt ttcggctgca gtaaacgctt tgccgtagta aggttgccat
    10621 ttcacatacc agagaccggc aatgacaacg agaaagaaaa gcgcgggttt ccaccactga
    10681 atgggcgttg ccgcctgaga tgaagactga ccagtcatag cattccccag gaataagtta
    10741 tgataattga gcgcgtgaat attacgctca ctatcaattc ttgggaataa ttattcagct
    10801 cttttgcgta attctgaaga gcttaaaatc gtcacgcctt catgctcagg ccgcatcgcc
    10861 tctgccagca tgacccgcgc cacgtctctc gcatcaatgg atttccagtt acctggtaac
    10921 aaacggaaca gcggcgcaaa aagcgtttcg ttcatccgct gtttgctacg atcgcccagt
    10981 aacatcgacg ggcgagcaat ggtcaatttc tgccagttct gggcaattaa tgcttcttcc
    11041 atctcccctt tgacgcggtt atagaaaaac ggcgagtggg cattggcccc catagcactg
    11101 accaccagca tatgctgcgc gcccagccgc cgcccggtta atgcggtatc cactaccagc
    11161 gtgtaatcgg catgaataaa cgcctctttg ctccccgctt ctcgccgcgt ggtgccgaga
    11221 caacaaaaca caatgtcgat gggatcggtg acctgcgcca gcgcgtcggt cagttgcgga
    11281 tcatggggat tgaacactcc tggcatatcg cccaacggac gtcgcgtcgg cgcggctatg
    11341 gcgttaactt tcggttcgtt aatcaacatc cgcagcaggt gaccgcccac cagccccgtt
    11401 gagcctgtaa tcagtacctg actcatcttc gctcctttac agaattgtct gccttgcgct
    11461 ccccggttca tgcaccaggc ttaatagacc atgaggtaat tatccccgat tgtggaaaat
    11521 tcgcctcatc caatgcaaca acgcggagga agcatgagta agaaaattgc cgttttaatc
    11581 actgatgaat ttgaagattc agaatttact tcacctgcag acgagttccg taaagccgga
    11641 cacgaagtga ttactattga aaaacaagcg ggtaaaacgg tgaaaggcaa aaaaggagaa
    11701 gccagcgtga ccatcgataa atccatcgat gaagtgacgc ctgcggagtt tgatgccctg
    11761 ctgctaccgg gcggccattc accggattat ctgcgtgggg acaaccgttt tgtcaccttt
    11821 acccgtgatt ttgtgaatag tggcaaaccg gtgtttgcca tctgtcacgg cccgcagttg
    11881 ctgatcagcg ccgatgtgat tcgggggcgc aaactgaccg cagttaaacc gatcattatt
    11941 gatgttaaaa atgcgggcgc ggaattttac gatcaggaag tcgtggttga taaagatcag
    12001 ctggttacca gccggacacc ggacgatctg ccagcgttta accgcgaggc gttacgcctg
    12061 ctcggtgcct gagtcgcgta gccaaatcat ttttttacca aagcccagcg tgttgtcggt
    12121 gaatttgatt tcatcgagcc ggatttccca caccggggcc gacagcattc tggcaaccgg
    12181 aaagcgacga ttgtacgcct tgcgcgcgag gtcgctttcc tcaccttcca gcctgcggat
    12241 ctcaccttta aactgcacac cgcgaattaa cgctaccgtt ttcggctgac cgtttaccgt
    12301 tccggcaact gccgcctgcg gcccgctcat ctgcgcgtgg cgcgtttttt cttccgtcaa
    12361 aatgtagaag gcgactttct gcgcatcaaa aagataaaag gcattagcgc accagagttc
    12421 cccttcctgc tgcacacacc aggtgacaac atgttgtttt gctagccaac ggctgatggc
    12481 gatgagtgtt tccattgctg ttctctttta tactgtgggc ctgaccttaa catgcgatgc
    12541 agacgatgac accctggttt ctttacttga tccgtaccgc cgacaataag ctttataccg
    12601 ggatcaccac ggatgtcgaa cgccgctatc agcagcacca aagcggcaaa ggggcgaaag
    12661 cactgcgcgg gaaaggagaa ctgacgctgg cgttttctgc tccagtgggc gatcgttcgc
    12721 tggcgttacg ggcggaatat cgcgttaagc aactgaccaa acggcaaaaa gagcgtctgg
    12781 tagcggaagg cgcagggttt gcagagctgt taagcagtct gcaaaccccg gagattaaaa
    12841 gcgattgaaa tgctcgtgat actcaaccag gccggtaacg ccattcagcg cgtcatccgc
    12901 taaacgatgt acctggaagg cgctttcagt gcccggccag cggcaacgca gatcgtgatg
    12961 cgccgccagt tcaaagccga aacgactgta cagcgccgga tcgcccagcg tcaccactgc
    13021 ggcatagccg aactcattaa gcgaatcgag tccttcatag accagttggc gtgccagccc
    13081 ctgtccacgg tatttttcat caaccgccaa cggtgccatg ccgacccatt gcaggtcttc
    13141 gccctgcaca tcaaccggac taaatgccac atagccaatg acctgacctt cgtcatctgt
    13201 cgccaccagc cccagcgtca gaaagccatc ttcacgcaga tcgtgaacca gcttcgcttc
    13261 cgcatcactt tcgaatgagc gacgcagcag ggcatcaata cccggcgcat caatgggaat
    13321 ttctactcga attagcatgg ttcacctacc gatgtctgtt tggtttcagg cgcggttttc
    13381 attcccgcct caacaaaatc cgccagttgc agcagcatca tgcgtaaggc tttcggcatt
    13441 tgctccagtt caatggcgtc catcaggttt ttcacataca gccccagctc cgtatcgcct
    13501 tcaatcacca gccgacgctg gaagaagagc gtatccggat cttgtttacg cgccgcaatc
    13561 atcagcagat cgctggcgtc ggcactaaaa ctcacatcag cttgcgcgtt ctggctgacc
    13621 accagtttgc cattcaccac cgaggtaaac cattgcaggt caatatcacg cacatgaata
    13681 cttaaccagc ggccttcaag aaactccagc tcgccatcat ccagcgcctg acggaattgc
    13741 cagcttaaga cctgctcaag aacctggcgt tttagcgcaa atggcgtcag ttttaccggt
    13801 acactcaaca gagatggccc caaatgcaca atacgggaac gcagtttatc caacacgagt
    13861 tttactccct gtttcaacaa tcatcctatt ttgccatatc agaaaaataa catagcggta
    13921 taaatcaaca attccatatg aaattgctgc taccaccaat acaactttaa ctgccttaaa
    13981 tcaaaaattg tcgcagcaag gttaactaaa atcccagttc gttaacattt ttgcgttttg
    14041 atagcgcaac cttcaggaaa aattatggag ctgctctgcc ctgccggaaa tctcccggcg
    14101 cttaaggcgg ccatcgaaaa cggcgcagat gctgtttata tcgggctaaa agatgatacc
    14161 aatgcccgtc acttcgccgg ccttaacttt accgagaaaa aattgcagga agcggtgagt
    14221 tttgtccatc aacatcgccg caaacttcac atcgcgatta acacttttgc gcatccggac
    14281 ggttacgccc gttggcagcg cgccgtggat atggcggcgc agctgggtgc cgacgcgctg
    14341 atcctcgccg acctcgccat gctggaatac gccgccgagc gttatccgca tattgagcgc
    14401 cacgtatcgg tgcaggcttc ggcgaccaat gaagaggcga ttaactttta tcatcgccat
    14461 tttgacgttg ctcgcgtggt gctgccgcgc gtgttgtcga ttcatcaggt gaaacaactg
    14521 gcacgggtca cacctgtacc actggaagtc tttgctttcg gcagcctgtg cattatgtcg
    14581 gaaggtcgtt gctatctgtc gtcgtatctg acgggtgagt cgcccaacac cgtaggcgcg
    14641 tgttctccgg cccgtttcgt gcgctggcaa caaacgccgc aggggctgga atcccgcctg
    14701 aacgaagtgc tgatcgaccg ttatcaggac ggcgaaaacg caggttatcc gacgctatgt
    14761 aaagggcgtt atctggtgga cggcgagcgc tatcacgcgc tggaagaacc aaccagtctc
    14821 aataccctgg aactgctgcc ggagttaatg gcggcgaata ttgcttcggt gaaaattgaa
    14881 ggccgccagc gtagcccggc gtatgtcagc caggtggcga aagtctggcg tcaggctatc
    14941 gaccgttgta aggccgatcc gcaaaacttt gtaccgcaaa gcgcgtggat ggagacgctc
    15001 gggtcgatgt ccgaaggcac gcaaaccact cttggcgcat atcaccgtaa atggcagtga
    15061 gaaaagcaat gaaatattcc ttagggccag tgctgtggta ctggccaaaa gagacgctgg
    15121 aagaatttta tcagcaggcc gccaccagca gcgccgacgt gatttatctt ggtgaagcgg
    15181 tatgcagcaa gcgtcgggca accaaagttg gcgactggct ggagatggca aaatcgctcg
    15241 ccgggagtgg taagcagatt gtgctctcca cgctggcgct ggtgcaggca tcatctgaac
    15301 tgggcgaact gaaacgctat gttgagaacg gtgagtttct gattgaagcc agcgatctcg
    15361 gcgtggtgaa tatgtgcgct gaacgcaaac tgccgttcgt cgccgggcac gcgctgaact
    15421 gctacaacgc ggtgacactg aaaatattgc tcaaacaggg catgatgcgc tggtgtatgc
    15481 cagtggagct ttcccgcgac tggctggtga atctgcttaa tcagtgcgat gagctgggca
    15541 ttcgtaacca gtttgaagtg gaagtcctga gctacggtca tctgccgctg gcctactccg
    15601 cccgctgctt taccgcgcgt tcggaagacc gcccgaaaga tgagtgtgaa acctgctgca
    15661 ttaagtatcc gaacgggcgc aacgtgctgt cgcaggaaaa ccaacaagtg tttgtactca
    15721 atggcattca gaccatgagc ggctacgttt acaacctcgg taacgagctg gcatccatgc
    15781 agggcctggt tgatgtggtc cgcctgtcac cgcagggtac tgacactttc gcgatgctcg
    15841 acgccttccg cgctaatgaa aatggcgctg caccactgcc gttgacggcg aacagcgact
    15901 gtaacggcta ctggcggcgg ctggcaggac tggagctgca agcctaagta aatagctcac
    15961 tttgttaaca actttaacta ctctttaatg cagtattaaa gattaatcgg taacaaagtg
    16021 agctgttatg actgataaaa ccattgcgtt ttcgctactc gatctggccc ccattcccga
    16081 aggttcttca gcgcgagaag cattctccca ctctctcgat ctcgcccgtc tggctgaaaa
    16141 gcgcggctat catcgctact ggctggcaga acaccacaat atgactggca ttgccagtgc
    16201 tgccacgtcg gtattgatcg gctatctggc ggcgaatacc accacgctgc atctggggtc
    16261 tggcggcgtg atgttgccta accactcacc gttggtcatt gcagaacagt tcggcacgct
    16321 taatacactc tatccggggc gaatcgattt ggggctgggt cgtgctccgg gtagtgacca
    16381 acggacaatg atggcgctac gtcgtcatat gagcggcgat attgataatt tcccccgcga
    16441 tgtggcggag ctggtggact ggtttgacgc ccgcgatccc aatccgcatg tgcgcccggt
    16501 accaggctat ggcgagaaaa tccccgtgtg gttgttaggc tccagccttt acagcgcgca
    16561 actggcggcg cagcttggtc tgccgtttgc gtttgcctca cacttcgcgc cggatatgct
    16621 gttccaggcg ctgcatcttt atcgcagcaa cttcaaaccg tcagcacggc tggaaaaacc
    16681 atacgcgatg gtgtgcatca atattatcgc cgccgacagc aaccgcgacg ctgaatttct
    16741 gtttacctca atgcagcaag cctttgtgaa gctgcgccgt ggcgaaaccg ggcaactgcc
    16801 gccgccgatt caaaatatgg atcagttctg gtcaccgtct gagcagtatg gcgtgcagca
    16861 ggcgctgagt atgtcgttgg taggtgataa agcgaaagtg cgtcatggct tgcagtcgat
    16921 cctgcgcgaa accgacgccg atgagattat ggtcaacggg cagattttcg accaccaggc
    16981 gcggctgcat tcgtttgagc tggcgatgga tgttaaggaa gagttgttgg gatagtgtgt
    17041 cttaacgcgg gaagccttat ccgagctggc aacgctgtcc tacatagacc tgataagcga
    17101 agcgcatcag gcattgtgta ggcagcagaa atgtcggata aggcaccgct gattactgat
    17161 acaccggcag taaattaaag ctcgataaaa tatgcaccag tgcgttgccg acgccaaaca
    17221 ccagaatcag cgcaatcatc ggcttgccac cccagacgcg gaatttcggg ctgccaaagc
    17281 gtttacgcga tgcacgggct aacagcgccg gaacaattgc cgcccagatg gtagccgcta
    17341 aaccagcata accaatggcg tacaggaatc cgttcgggaa caacagcccc cccacaactg
    17401 gcggggcaaa ggtcagcaat gccgttttca agcggcccac agccgagtcg tcgaaaccaa
    17461 acagatctgc cagatagtca aacaaaccca gcgttacgcc gaggaacgaa ctcgctaccg
    17521 caaagtttga gaacacgacc agcagcagat ccagactacg gctgttcagt acgccgctta
    17581 acgcctgtac cagcacatca atattaccgc ccttctctgc aataccgata aactccggac
    17641 gcgggatgtt acccatcgtc gccagcaacc agatggtata cagcgccagc gccatcagcg
    17701 taccgtacac cagacatttc acgatggttt tcggatcttt gccgtaatac ttcatcaggc
    17761 ttggcacgtt accgtgataa ccaaacgatg ccagacagaa cggcagggtc atcaacagat
    17821 acggtgcata agacgcattg ctttcggcga cgttgaacaa tgtcgcaggc tgcacatgcc
    17881 ccagcaggct accaaaggtg aggaagaagg taatgacttt cgcccccagc acaatcgctg
    17941 tcatgcgact gacggcttta gtgctcaacc acaccacaaa cgctaccagc aatgcaaaac
    18001 caaaacccgc cgcccgtgcc gggacgttta gtgacatctc tgcgaaggtg tgatgcagaa
    18061 tcgaaccact ggcagaaata taggcatagg tcaggatata gagcacaaag gcaatggaaa
    18121 tgccgttgac cacgttccag cctttgccca gcaaatcttt ggtgatggtg tcaaaactcg
    18181 aaccgattct gtaattcagg ttagcttcca gaatcatcaa gccggaatgc agcatacaga
    18241 accaggtaaa gatcagcgcc gccattgacc agaaaaacca cgccccggac atgaccactg
    18301 gcagagaaaa catccctgcg ccaataatgg tgccgccgat aatcaccacg ccgccaagca
    18361 gcgacggtga cgtttgggtg gtggttagtg ttgccatgag ggcttctctc cagtgaaaaa
    18421 tagtgcgact gcgttgttat gcattgcact gtaccagtac acgagtacaa aagacagaaa
    18481 aaaagccccg atggtaaaaa tcggggctgt atatattatt ttacagattg tgttcgctgt
    18541 tcagcgatga ttacgcatca ccaccgaaac gacgacgacc ggtagaatca tcacgacgcg
    18601 gagcgcggcc ttcacgacgt tcgccgctaa aacgacgacc atcaccacgg ccaccttcac
    18661 ggcgttcacc gctgaagtta cgaccgcctt cacgacgttc gccaccgaaa ccacgaccac
    18721 cgccacgacg ctcaccgcca gtatgcggct gtgcatcgcc cagtaactgc atgttcatcg
    18781 gcttgttgag aatgcgagtg cgcgtaaagt gttgcagcac ttcacccggc atacctttcg
    18841 gcagttcgat ggtggagtga gaagcaaaca gcttgatgtt accaatgtaa cggctgctga
    18901 tgtcgccttc gttagcaatc gcaccaacga tatgacgaac ttcaacacca tcatcgcggc
    18961 ccacttcaat gcggtacagc tgcatatcgc caacatcacg acgttcacga cgcggacgat
    19021 cttcacggtc accacgcggg ccacggtcgt tacgatcgcg cggaccacgg tcatcacggt
    19081 cacggaattc acgtttcgga cgcatcggcg catctggcgg tacgatcaga gtacgttcac
    19141 cctgtgccat tttcagcagt gccgcagcca gagtttcgag atccagctct tcaccttcag
    19201 cagtcggctg aattttgctc agcagtgcgc ggtattgatc cagatcgctg ctttccagct
    19261 gctgctgtac tttagcggcg aatttttcca gacggcgttt gcccagcagt tctgcgttcg
    19321 gcagttctac ttccggaata gtcagcttca tagtacgttc aatgttgcgc agcagacgac
    19381 gctcgcggtt ctcaacgaac agcagcgcgc ggccagcacg acccgcacga ccggtacgac
    19441 cgatacggtg aacgtaagac tcagaatcca tcgggatatc gtagttaact accaggctga
    19501 tacgctcaac gtccaggcca cgggccgcaa cgtcggtcgc aatcaggatg tccagacgac
    19561 catctttcag gcgttccaga gtctgttcac gcagcgcctg gttcatgtca ccgttcagcg
    19621 cggcgctgtt gtagccgtta cgctcaagag cttctgccac ttccagagtc gcgtttttgg
    19681 tacgaacgaa gataatcgcc gcatcaaaat cttccgcttc caggaaacgt accagtgctt
    19741 cgtttttacg cataccccag acagtccagt agctctggct gatgtcagga cgggtagtca
    19801 cgctggactg aatgcgcact tcctgcggct ctttcataaa gcggcgggta atgcgacgaa
    19861 tcgcttccgg catggttgca gagaacagag cggtctgatg accttccggg atctgcgcca
    19921 taatggtttc aacgtcttcg atgaagccca tgcgcagcat ttcgtcagct tcatccagaa
    19981 ccagaccgct cagtttagag aggtccagag tgccacgttt caggtggtcc agcagacggc
    20041 ccggagtacc gacaacgatc tgcggcccct gacgcagggc gcgtaattgc acgtcataac
    20101 gctggccgcc gtacagggca accacgttta cgccgcgcat gtgtttagag aaatccgtca
    20161 ttgcttcagc aacctgtacc gccagttcgc gggtcggtgc cagcaccaga atctgtggtg
    20221 ctttcagctc aggatcaaga ttttgcagca gaggtaaaga gaatgctgca gtttttccgc
    20281 tccccgtctg ggccataccc agaacgtcgc ggccattcag cagatgtgga atacactctg
    20341 cctgaattgg agatggtttt tcgtaaccca gatcgttaag ggcttcaagg ataggagcct
    20401 tcaggcccag atctgcaaaa gtggtttcga attcagccat gtagtacgtg tgcctcaaaa
    20461 ttaatggcgg ccagtctaca taactcatca tgaaattgat cagcaatttt cattgaaaag
    20521 tgtgaaccgg ctcaaagtag gtgtattaac gaacaacaac gccctcacca gttaaggtga
    20581 tggcaatcaa aaaagattac gggctgatgt gtacgtcagc tattgctggt ccgattctgc
    20641 caggtcatct tggtcctggc ccaggagcga taattccaac aatgcgtatc ggtgctcaac
    20701 aaagttatga acgttgttgg caaccgccag tttgaacagt gccgtggcgc tgtccaaatc
    20761 ccccagactt aggtagtact tacctaaata gaagttggtt tcactgagat gctcagcgag
    20821 cgaggtgtta tccgttgcgt ccgccttgag cctttccatt aacgtttgtt cgctaatgtt
    20881 gcccaggtag aactcgacaa tgttccatcc ccactgttcc ttatccgatt tttcgaagtg
    20941 ctgtttcaac acttctttag cctgcttctc atcgagcttc tgctcggcga gataaagcca
    21001 cagactacgg aaaggatcat tgggatcgtc ttgataaaac gccagcagat catcttgcgc
    21061 taacttgtca cgaccgccgt aatataatgc gatcccgcga ttcaagtgcg cgtagttgta
    21121 agttggatca agctcaagta cagaatcaaa cgcttcatag gcagcatcaa aattgcctgc
    21181 ctgcgttaaa tatatgccta agtaattgaa tacttcaggc atatccggtc ggattgccag
    21241 cgcttgcgaa aaatcgttac gcgctaatgc cctcagaccg agactatcat acaacactcc
    21301 gcgctcatat aaaagctgtg cgcgttcgtc atcggttaaa gcccgactgg caaggatttg
    21361 ttccatacgt gccagaatca cttcctgctg taaagtcggt tgcaatggta ccgcgaggac
    21421 ttcactttta cgccaggaag tattactgca tcctgcaagc gtaagtgctg tcgcaacgaa
    21481 acaccagcgc aaaaaaggct tcatttccca ctcccgaaga ccacggttga atgaacgtcc
    21541 tgttcccggt tgctaacaag gcgtcctgcc cggttaaaag ccccccgccg cagcggaggg
    21601 caaatggcaa ccttactcgc cctgttcagc agccggagct tccggtgctg cagcaggttg
    21661 agactgctca gtcgcttctt taatgctcag acggatacgg ccctggcgat caacttccag
    21721 aactttcacc ggtacttcct gacccatctg caggtaatcg gtcactttct caacgcgttt
    21781 gtcagcgatt tgagagatgt ggaccagacc ttctttaccg ccgccgatgg caacaaatgc
    21841 gccaaagtca acgatacggg tcactttacc agtgtagacg cggcccactt cgatttctgc
    21901 agtgatctct tcgatacgac gaatagcatg tttcgctttc tcgccgtcgg tcgctgcgat
    21961 cttcacagta ccgtcatctt cgatttcgat ggtggtgccg gtttcttcgg tcagggcacg
    22021 gataacagag ccgcctttac cgataacatc tttgatcttg tccgggttga tcttgatggt
    22081 atggatacgc ggtgcgaact cagagatatc gccacgcggc gcgttgatcg cctgttccat
    22141 tacgcccagg atatgcagac gcgcaccttt agcctggttc agcgcaacct gcatgatctc
    22201 tttggtgata ccttcaattt tgatatccat ctgcagtgca gagataccgt cgcgggaacc
    22261 tgcaactttg aagtccatat cgcccaggtg atcttcgtcg cccaaaatgt cagacagtac
    22321 aacgtagttg tcgccttctt tcaccagacc cattgcgata cccgcaacgg cagctttgat
    22381 cggcacacct gcgtccatca gcgccagaga cgcgccgcac acggaagcca tagaagagga
    22441 accgttggat tcagtgattt cagacacaac acgtacggtg tacgggaatt tgtccatatc
    22501 cggcatgact gccagcacgc cgcgcttcgc cagacgaccg tgaccaattt cacgacgctt
    22561 cggagaaccg accatgccgg tttcgcctac ggagtacgga gggaagttgt agtggaacag
    22621 gaaggtatcg gtacgttcgc ccatcagttc atcaagaacc tgcgcgtcac gagcagtacc
    22681 cagcgttgcg gtaaccagcg cctgcgtttc accacgggtg aacagcgcag aaccgtgagt
    22741 acgcggcagc acgccagtac gcacatccag accacggatc atatcttttt cacgaccgtc
    22801 gatacgcggt tcgcctgcca gtacgcggct acgaacaacg tttttctcga tcgcgtgcag
    22861 aatttcaccc agttcgtttt cgtccagggt ttcgtcttca gcaagcagcg tcgcgatggt
    22921 ttcagatttg atgacatcaa cctgcgcata acgctcttgt ttgtcggtga tgcggtaagc
    22981 atcgctcagg cgagcttcag ccagtgcagc aacgtgcgcg tttagcgctt cgtttaccgg
    23041 ctccggctgc cagtcccaac gcggtttacc ggcttctttc accagttcat tgatgttctg
    23101 aataacaacc tgctgttgtt catgaccgaa cactactgcg cccagcatct ggtcttcgct
    23161 cagcagttga gcttcagatt caaccatcag tacagcggct tcagtaccgg caacaaccag
    23221 atccagtttg ctctctttca gctcgtcctg agtcgggttc agtacgtact ggtcattgat
    23281 gtaacctacg cgggcagcac caatcgggcc attgaacgga ataccagaca gagacagcgc
    23341 tgcggaagca ccaatcatcg cgacgatatc cgggttaact tgcgggttaa cagaaaccac
    23401 ggtggcgata acctgaactt cgttgacgaa gccttccggg aacagcgggc gaatcgggcg
    23461 gtcaatcaga cgcgcgatca gggtttcgcc ttcgcttggg cggccttcac gacggaagaa
    23521 gctacccggg atacgaccag cagcgtaggt acgctcctga tagttaacgg tcagtgggaa
    23581 gaagtcctga cctggtttgg cttttttctg gccaacaacg gtaacgaata ccgcggtgtc
    23641 atccatgcta accataacag cggcagtagc ctgacgagcc atcatgccgg tttccagagt
    23701 cacggtgtgt tggccgtact ggaatttacg aacgatcgga ttaagcaatg taatatcctt
    23761 tctctttctt agacagtacc ttacggcact ggtgttaata cccgatcttc tgcgcatcct
    23821 cgcgactaat gacaacccta acccagctct atgtgggtaa agcctctcat tagccgcgcg
    23881 aacctctgca acggaagatc attcatagca acaatacatt agtttccagt gaattgctgc
    23941 cgtcagcttg aaaaaagggg ccactcaggc ccccttttct gaaactcgca agaattagcg
    24001 acgcagaccc aggcgctcga tgagctgggt gtaacgtgct acgtctttac gtttcaggta
    24061 gtcgagcagt ttacgacgct gagaaaccat gcgcagcaga ccacgacggc tgtggtgatc
    24121 ttttttgtgc tctgcaaagt ggccctgcag gtggttgatc tgtgcagtca gcagtgctac
    24181 ctgaacttcg gtagaaccgg tgtcgtttgc gtcacgacca aactcagaaa cgattttagc
    24241 tgttgcttca gtacttagag acattttaaa actccaaagt atatagaatg aaaggacgcc
    24301 gatctctaat tcagcgatcc cagtgtacgt tacgcaaagt gttaaacaat ttacgcgacg
    24361 ttaagcggca gtattctact cgtagcgacc tgttatcgca agacggttaa cattacgccg
    24421 ggtatttaac caccaggcga cgaggcgcaa cgcggccttc atcgtcaatt tcgcccatac
    24481 cgataaattt gccgttctca ccttccgtga cgcgaaccag tccttccagt ggcgcaccag
    24541 atgtacgaac cgggttacca tttttgaagt aaacagaaga cgttaacgga agattcacca
    24601 ccggatagtc cgaagctgga ctgtccattg gcatcagtaa tggatcaagt aactccgcgg
    24661 ctggaatatc ctgctgttca gcttgctcaa caagttcacg caggtgctcc agggtcacca
    24721 tccgttcaac cggatattta cttaccgcca gacggcgcag gtaaataaca tgcgcgccac
    24781 agccgagttt ttcacccagg tcatcaatga tggtgcggat ataagtgcct tttgagcagt
    24841 gaatttccag ctccagctca ttgccttcat ggcgaataaa cagcaattca taaacggtaa
    24901 tcggacgcgc ttcacgcgga acttcaatgc cctgacgcgc atattcgtac agttttttgc
    24961 cctgatattt cagtgctgaa tacatcgaag ggatctgttc gatatcgcca cggaaagtat
    25021 ccagtgccgc tgccagctgc tctgcgctaa aggttaccgg acgttcttca acgatctgtc
    25081 cgtcggcatc agaagtatcg gtacgctgtc caagacgcgc aatgacccga tagcgtttgt
    25141 cggagtccag cagatactgg gaaaacttcg tcgcttcccc gaggcaaatc ggcaacatgc
    25201 cggtcgccag cgggtccagc gcaccggtat gcccggcacg gttggcgtta tatatacgtt
    25261 tcactttttg cagcgcatcg ttgctggaca taccctgagg tttatccagc aacaaaacgc
    25321 cgttaatgtc gcgaccgcga cgacgaggac gactcattag tcctccttgc tgtcgtccgg
    25381 gttaacacga cgttcttcgt catgtttgac cacgctggtc accaggtttg acatgcgcat
    25441 cccttcaacc agagagttgt cgtagaagaa ggtcagttcc ggcacgatac gcaggcgcat
    25501 cgctttcccc agcaggctgc ggatgaaacc agaagcttct tgcaacgctt tgatgcccgc
    25561 tttaaccgcg tcttcatctt tgtcgttgag gaacgtcaca tatactttgg catacgccag
    25621 gtcgcgagac atttcgacac cggaaacggt ggtcatcatg cccaggcgag gatctttaat
    25681 ttcacgctgc aggatgagag cgatctcttt ttgcatttcc tgcgctacgc gctgcgggcg
    25741 accaaattct ttcgccataa taaattctcc tgacaaaaaa ggggctgtta gccccttttt
    25801 aaaattaatt tcaggtggaa gggctgttca cgttgacctg ataagacgcg ccagcgtcac
    25861 atcaggcaat ccatgccgga tgcagcgtaa acgccttatc ccgcatggaa ccctaaaaac
    25921 cttaagcaat ggtacgttgg atctcgatga tttcgaatac ttcgatcaca tcgccagtgc
    25981 ggacgtcgtt gtagttctta acgccgatac cacattccat accgttacgg acttcgttaa
    26041 cgtcatcttt gaagcggcgc agggactcca gctcgccttc gtagataacc acgttgtcac
    26101 gcagaacgcg gatcgggttg tgacgtttaa ccacaccttc ggtaaccata cagcctgcga
    26161 tggcaccaaa tttcggcgat ttgaacacgt cacgaacttc cgccagaccg ataatctgct
    26221 gtttcagttc cggagacagc ataccgctca tcgccgcttt cacttcgtca atcaggttat
    26281 agatgacgga gtagtaacgc agatccaggc tttccgcttc aatcacttta cgtgcagagg
    26341 catcagcacg tacgttaaag ccaaccagga tggcgttgga cgccgcagcc agggtggcgt
    26401 cggtttcggt gataccacct acgccagaac cgatgatctt cactttaact tcgtcagtag
    26461 acagtttcag caaggagtcg gagatcgctt cgacagaacc ctgtacgtct gccttcagga
    26521 cgatattcac ttcgtgaact tcgccttcgg tcatgttggc gaacatgttc tcgagtttag
    26581 atttctgctg acgcgccagt ttaacttcgc ggaatttacc ctgacgatag agtgcaactt
    26641 cacgcgcttt cttctcgtca cgtacaacgg taacttcatc acccgcagcc ggtacgccgg
    26701 acaggccgag gatttccacc ggaatggacg gacccgcttc cagcacttcc tgacccagtt
    26761 cgttacgcat cgcacgaaca cgaccgtatt cgaagccaca cagaacgata tcgcccttgt
    26821 gcagagtacc ttcacgtacc agaacggtag caaccggacc acgaccttta tcgaggaagg
    26881 attcgataac cgcaccgctc gccatacctt tacgtaccgc tttcagctcc agaacttccg
    26941 cctgcagcag gatagcgtcc agcagttcat cgataccggt acccgctttc gcagatacgt
    27001 gtacgaactg gctttcaccg ccccactctt ccggcaggat gccgtactgg gagagttcgt
    27061 tcttaacgcg atccggatca gcttctggtt tatcgatctt gttcactgca accaccaccg
    27121 gtacctgcgc cgctttcgcg tgctggattg cttcgatggt ctgcggcatc acaccgtcgt
    27181 cggcagcaac aaccaggact acgatgtccg ttgcctgcgc accacgagca cgcattgaag
    27241 taaacgcggc gtgccccggg gtgtccagga aggtgatcat gccgttttca gtttcaacgt
    27301 ggtatgcacc aatgtgctgg gtaatgccgc ccgcttcgcc agaggccact ttcgttgaac
    27361 gaatgtagtc cagcagagag gttttaccgt ggtcaacgtg acccatgatg gtcacaaccg
    27421 gcgcgcgcgg ttcagccgca gcacccgtgt cacggtcgct cattaccgcc tcttccagct
    27481 cgttttcacg acgcaggata actttatggc ccatctcttc agcaaccagc tgtgcggttt
    27541 cctgatcgat aacctggttg atggttgcca ttgcgcccag tttcatcatc gctttgatga
    27601 cctgagagcc tttaaccgcc atcttgttcg ccagttcgcc aacggtgata gtttcgccga
    27661 tcacaacgtc acggttaacg gcctgagcag gcttctggaa gccttgctgc agcgaagaac
    27721 ctttacgttt tccgccttta ccgccacgta ctgctgcgcg tgcttcttca cgatcagctt
    27781 ttgattcagc gtgtttgttg cctttcttcg gacgcgctgc tttcgcgtta cgaccacggc
    27841 cacggccgcc ttcgacttca cgatcgcttt cgtcttctgc ctggcgagca tgttgagaag
    27901 tagtgacgtg ataatcgctg gaatcttcag tcggttccgc gttatcagtc catttgtttt
    27961 cttccgccat acgacgtgct tcttcagcaa cgcgacgtgc ttcttcttcg agtttacgac
    28021 gcgcttcttc ttcagcttta cgcttgagct ctgcagcttc ctgctcacgg cgggcttttt
    28081 cagcctgggc gtttttagtc atatcgtctt gttgattgct cactttgtct ttttccgcag
    28141 cttcacgttt cgcttgttca gcagcttcac gcttagcttg ttctgcggcc tcacgttcag
    28201 ctttttgttg cgcctcgcgt ttagccgatt cttctgcctc acgacgggct tgctcttccg
    28261 cttcacgctg cgcttgctct tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca
    28321 caaaggtgcg tttcttgcgg acttcgattt gtaccgattt gctttttcca ccggtaccag
    28381 gaatgttaag ggtgctgcgt gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt
    28441 tctgattcag gtggtcaatc aaagtctgtt tctcttgtgc agacacagag tcgtcagcag
    28501 acttccggat acctgcatca gcaaattgct gtaccaggcg ttccacggag gtctgtcgct
    28561 ctgcggccag cgttttaatc gttacatctg tcatgctgtt ccttcctgct acagtttatt
    28621 acgcttcgtc accgaaccag caaatattac gggcagccat aatcagtgct ccggcttttt
    28681 cgtcggtcaa cccttcgata tcagccagat catcaatgcc ctgttcggcg agatcttcca
    28741 gcgtacaaac gccacgggcg gccagtttga atgccaaatc acgatctacc ccttcaaggt
    28801 tcagcagatc gtcagccggt ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg
    28861 tggccagtgc atttttagca cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt
    28921 cgatttccaa cagctctttc atcggcacat aggccagttc ttccagcgtc gagaagcctt
    28981 cttctaccag aacagtcgcg aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga
    29041 tcgctgcgtg cgcttccgcc tgatgcttag cctgcaggtc gtcaacggtc atcacgttga
    29101 gttcccaacc gctcagctgc gaagccagac gcacgttctg accgttacgg ccaatcgcct
    29161 gcgccaggtt accggcttca acggcgatat ccatggtgtg tttatcttca tccaccacga
    29221 tagaagcaac gtctgccggt gccattgcgt taatcacgaa ctgcgccggg ttatcatccc
    29281 acaggacgat atcgatacgc tcgccaccca gttcagtaga caccgcctga acacgcgcgc
    29341 cacgcatacc tacgcaagca cctaccggat cgatacgttt atcgttggtt ttcactgcga
    29401 ttttcgcacg agaacccgga tcgcgagccg ctgctttaat ttcaatcact tcttcgccga
    29461 tttctggcac ttcaatacgg aacagttcga tcagcatttc cggcttggaa cgagtgacga
    29521 acagttgcgc gccacgcgct tccgggcgaa cggaataaag cacgccacga acgcggtcgc
    29581 cagggcggaa gttttcacgc ggcagcatat cttcacgcag gatcacggct tcagcgttgt
    29641 tgcccagatc cagagagatg ttgtcgcggt ttactttttt caccacgccg gtgatgattt
    29701 caccttcgtg ttcacggaac tgatcaacca ccatcgcacg ttcggcttca cgcactttct
    29761 gcacgataac ctgttttgcc gtctgggtag tgatacggtc aaaggtaaca gactcaatct
    29821 gatcttcaac gtaatcgccc aggttcaggc tttcatcttc ataacgtgcg gcttcaaggg
    29881 tgatttcctt ggtcggctgg gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa
    29941 aatcaccgct tttgcgatcg atctgtacgc ggacgtcgat ctcttgttca tattttttct
    30001 ttgttgctgt cgccagcgcg ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct
    30061 tttcattgga tacggcttca actacagcca aaatttcttt gttcatcgcg ggcttttcac
    30121 ctcatccaga ctattaaaag tggggaacca ggttcgcctt ctggatatta ctcagcgcga
    30181 acacttcatc tttaccttcg acggtaactg tgatcatttc accgtctacc gctttgataa
    30241 cgccctgcca tttacgacgg ttttgtaccg ccatacggag aaccagagtc acctcttctc
    30301 cgacaaaacg ggcgtagtgt tcagccgtga acagtgggcg atcgagaccc ggtgaggaga
    30361 cttccaggtt ataagcaacg gtgatgggat cttcaacatc cagcacagca cttacctggt
    30421 ggctcacatc agcacaatca tcaacattga tgccatcttc actatcaata tagatgcgca
    30481 gtgtggatgt gcgaccgcga ataaattcga tgccaaccag ttcaaaaccc agggcctcaa
    30541 ctggcgcagt aatcatctct gttaattttt gctctaatgt ggacaagccc acccccaaga
    30601 cataaaaaaa gggcctaaag cccagttatt ctgtagtcag ataacaaaaa accccgataa
    30661 atcggggctt tatataactg aaccctataa ccgcaactgc ggtctggagc actttccaga
    30721 aggatttttt caaatcccac tacgaaggcc gaagtcttca cagtatattt gaaaaagaac
    30781 tctaagggaa agtggttgcg ggggccggat ttgaaccgac gaccttcggg ttatgagccc
    30841 gacgagctac caggctgctc caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg
    30901 gtaaaaaatg caaatactgc tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg
    30961 cattatagtg atccacgcca cattttgtca acgtttattg ctaatcatgt gaatgaatat
    31021 ccagttcact ttcatttgtt gaatactttt gccttctcct gctctccctt aagcgcatta
    31081 ttttacaaaa aacacactaa actcttcctg tctccgataa aagatgatta aatgaaaact
    31141 catttatttt gcataaaaat tcagtgagag cggaaatcca ggctcatcat cagttaatta
    31201 agcagggtgt tattttatga cgacgattct caagcatctc ccggtaggtc aacgtattgg
    31261 tatcgctttt tctggcggtc tggacaccag tgccgcactg ctgtggatgc gacaaaaggg
    31321 agcggttcct tatgcatata ctgcaaacct gggccagcca gacgaagagg attatgatgc
    31381 gatccctcgt cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg actgccgcaa
    31441 acaactggtg gccgaaggta ttgccgctat tcagtgtggc gcatttcata acaccaccgg
    31501 cggcctgacc tatttcaaca cgacgccgct gggccgcgcc gtgactggta ccatgctggt
    31561 tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac ggtagcacct acaaaggaaa
    31621 cgatatcgaa cgtttctatc gttatggtct gctgaccaat gctgaactgc agatttacaa
    31681 accgtggctt gatactgact ttattgatga actgggcggc cgtcatgaga tgtctgaatt
    31741 tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa aaagcctact ccacagactc
    31801 caacatgctt ggtgcaacgc atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa
    31861 aatcgtcaac ccgattatgg gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga
    31921 agaagtcaca gtacgctttg aacaaggtca tccggtggcg ctgaacggta aaacctttag
    31981 cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat
    32041 gagcgaccag attgaaaacc gtatcatcga agcgaaaagc cgtggtattt acgaagctcc
    32101 ggggatggca ctgctgcaca ttgcgtatga acgcctgttg accggtattc acaacgaaga
    32161 caccattgag cagtatcacg cgcatggtcg tcagttgggc cgtctgctgt accaggggcg
    32221 ttggtttgac tcccaggcgc tgatgctgcg tgactctctg caacgctggg ttgccagcca
    32281 gatcactggt gaagttaccc tggagctgcg ccgtgggaac gattattcaa tcctgaatac
    32341 cgtctcagag aacctgacct acaagccaga gcgtctgacg atggaaaaag gcgactcggt
    32401 gttctcgcca gatgatcgta ttggtcaatt gaccatgcgt aacctggata tcactgatac
    32461 ccgcgagaaa cttttcggtt atgccaaaac tggcctgctt tcctcctctg ccgcttcagg
    32521 cgtgccgcag gtggagaatc tggaaaacaa aggccagtaa ttcgacatca accctgccct
    32581 tcaggggtgg ggttaccttg cttatcctct gtaaactcct ttctgagcat cacataatcg
    32641 aaaacatcat ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc aatcacttcc
    32701 tcaagcgttt gcggctgttc cggctttgtt acccccatcc acgcattaat aaggtaatta
    32761 ttgtaagctg tcgaaaagat gttgttttcc gttgtgcgat ccacgccatc cccaagaacg
    32821 gggctatacc agataaacat cgggacatga tatgcctgct ggctagcctc cctgcctcca
    32881 tgaaaataga cgttcttctt cgtagggtca cgttccaggc cgtggtcggc aaaatacatg
    32941 accgaggcgc ggcgatcttt taataattca aaaacctgac ctagcaaact atctgtgtaa
    33001 tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat
    33061 tgcggatagg cgctacaagc cggttcatgg cttccattta aatgaagaac aatcagtttc
    33121 ttttgctgcg tattttgctg taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat
    33181 cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc tgtaccattc
    33241 tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg attagccata
    33301 ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc agcagttaaa
    33361 gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg
    33421 atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc atacaaagac
    33481 atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat acctgtatcc
    33541 ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct ttgatgctct
    33601 ttcgctgcta aagcaaaata gttgagattg aaaaacggcg tataggtagc aaatcgcgac
    33661 gctagtatat atggactgaa cgctttttta ttttttgcat ctttataagc aaattgacaa
    33721 gcggaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac tttttttgtc
    33781 ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa aaaagcaaag
    33841 gcacatagat aagggatata catccccagc attttgacaa cttcatccgg atcactctgg
    33901 agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca tgaatagagg
    33961 ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag caggcgaagc
    34021 ggaagggatg atatacgttt tacaaccagg atggtcagaa cgaagaacag caagcgctga
    34081 accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat aacacaaaga
    34141 tacaacaacc aatgagattt aaaacttcta gcaaatttgt tgaatactgt catccagaaa
    34201 tccaagttca caaccaatat aaacaattat caacaatgtt caattttata tcagacatgt
    34261 ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt taaatgatgc
    34321 ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac attacgcaga
    34381 acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa gtaaacatat
    34441 ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc agcagaaggg
    34501 aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct acgagtaaag
    34561 caactggacg agatacagat acctgacaac cattcctcag accaggacca aaacgaaaaa
    34621 agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact tgaacccgta
    34681 agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc acctcggcac
    34741 ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag ctggttgagt
    34801 ttgttcggtt ttcaccggtg cactcagatt ttcccattcg ctacctttat tggttttgtt
    34861 gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca gcagcgccgt
    34921 catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg aagcgcctgc
    34981 tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca gaccaacaag
    35041 gccaattgcc acaataagga aaactactaa aagagcttcg tacataatca acctgttcct
    35101 tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta atctttccca
    35161 ctgaagcggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa ttttatcgca
    35221 ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa aaaggcgaca
    35281 atcgccgcct ttttagccag ttatctaacg ctttaaacgg cttttactgc atcggcgatg
    35341 cggtgtgcaa attcagtcac ctgcgcttcg tcttcgcctt ccaccatcac gcgaattaac
    35401 ggttcggtgc cggatttacg cagcaacacg cgtccacggt tgcccagcgc agcttcaacc
    35461 tctgcggtca cggctttaac tgactcatgc tcaagtggat cgccgctacc tgcggtgtaa
    35521 cgtacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc gtgcaggctc
    35581 atatggttac gtgccatagc cgccagcacc tgcaagccag caacgatgcc gtcaccggta
    35641 gtagttttat ccagcaggat cacatgaccg gaattctctg caccgatacg ccagcctttc
    35701 tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa tggaattcct
    35761 aactgtttca gcgccagttc aagccccatg ttgctcatca atgtacccac agcgccacca
    35821 cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg atcgccatcg
    35881 actttattgc cttcatggtc aaccataatc acgcgatcgc catcgccgtc gaaggcaata
    35941 ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc ggtagccccc
    36001 acttcggcat tgatgtttac accgtttggc tcacaaccga tagcgataac gttcgccccc
    36061 agttcgcgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcaca atccaccaca
    36121 atcttcagtt cactgaggct aagttcgttc gggaacgtgg ctttgcaaaa ctcgatatag
    36181 cgacccgcgg catcaacgat acggctggct ttacccagtt ctgccgaatc aacgcagctg
    36241 atctcctttt ccatttccgc ttcgatggcc tcttctaccg catccggcag tttggtgccg
    36301 tcgatagaga agaatttaat gccattatcg tagaacgggt tatgcgatgc agatatcaca
    36361 attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt tggcatcggg
    36421 ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc tgactccagc
    36481 atatagccag aaatacgcgt gtctttacca ataatgatct tacgggagcc gtggcgcgcc
    36541 agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat cggcgcatcc
    36601 cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat agcgtttgtt
    36661 ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt ctttgacgtc
    36721 atgaacacga ataatgtgcg cgccttgcat tgcggcaatg accgcacagg ccagactacc
    36781 gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt ttcgtgacat
    36841 acctaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca gtaatgaata
    36901 gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt tctcttttgc
    36961 gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca cttctgcaaa
    37021 gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca tatgcatcag
    37081 acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg aaagggagcg
    37141 gatatcatta ataatgtgag cgccaacttt cgctgactca cggatgactt ctggtttgga
    37201 tgtatctacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca caggaataac
    37261 acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg actcgccacc
    37321 aacgtcaatg atcgtcgcgc cagcgttgat catcagattc gcatgtttca ccgcatctat
    37381 cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga ggatccccat
    37441 tacgtgagga tggctaaggt ccagtgaagt accctgggca aagagtttca tgatgttatc
    37501 cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc ggtacaaata
    37561 cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt gttacccggg
    37621 ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt gtcgccagaa
    37681 ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg acgtgccatc
    37741 aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc tttcatcgca
    37801 tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt acgctcaatc
    37861 agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg tttcgctttc
    37921 gctacgctac ggccgaggaa tacttcacct tcttcttccg cgtacagcag tggacccaat
    37981 ttctcagaga agccccactg agtcaccatg ttacgtgcca ggttggtcgc aactttaata
    38041 tcgttggacg caccggtaga aacatgttcc ggcccgtaga tgatctcttc tgccagacga
    38101 ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct gattgcgtcg
    38161 ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat cgtcactttg
    38221 tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc ttcgtggtaa
    38281 gccgttgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc acccatcatg
    38341 attttgtctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt gccacgagca
    38401 gcgaacagtg ccgcttcgtt caccaggttc gccaggtcag caccggagaa accaggagta
    38461 ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg catgtgaact
    38521 ttcaggatct gctcacgacc gcgaacatct ggcaagccaa ccacaacctg acggtcgaaa
    38581 cggccaggac gcagcagggc cgggtcgaga acgtccggac ggttagtcgc ggcgataacg
    38641 atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt cagagtctgt
    38701 tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac ggcgtcgatt
    38761 tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat gtcacgaaca
    38821 cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat agtaaagaac
    38881 ggaactttcg cttcgcctgc aatcgctttc gccagcagcg ttttaccggt acccggagga
    38941 ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg gctcggctcg
    39001 cgcagatact caaccagttc agcaacttct tcttttgctt cgtcgcagcc cgcaacgtca
    39061 gcaaaggtcg ttttgatctg atcttccgtc aacatgcgcg ctttgctctt accaaacgac
    39121 atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca gacaccaatc
    39181 agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg ttcttcaggc
    39241 ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt cggatcctga
    39301 accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat ttcacgtccg
    39361 ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt agagtaatcc
    39421 accttacggc cattagactc gctgggccca aagctctgga atactgacat cagcacaacg
    39481 gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt aacctcttat
    39541 tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc aaactttcgt
    39601 ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc ccgcgaacgt
    39661 gcacgagaag agtccggctt acgaacttta accttcgtaa acagggagcg aatttccctt
    39721 agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc acctggcgct
    39781 aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg ggggatatcc
    39841 accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg gactttgctg
    39901 tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc gccctgaaga
    39961 aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat gcggcctttg
    40021 ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc aaggtcgaca
    40081 accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag tttaaaccag
    40141 gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct aaagtgttcc
    40201 tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact ttcccatggg
    40261 gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga tatatgggag
    40321 atggcggtag aatgacccgt tttcaatccc aacgtaagca aaaaatacga tgaatctgag
    40381 tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag ttgttctgct
    40441 tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt tagagcacca
    40501 tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa actaaaacct tgatcgtgga
    40561 agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa cgctggtgct
    40621 ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc ctaaagttac
    40681 acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa tgccacgctc
    40741 tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata catcaacatc
    40801 ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt ctacttcgcc
    40861 gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc cacgagcaat
    40921 aggcgagttt acagaaatca ggttttgttt aaagtcagct tcatcatcgc caacgatgcg
    40981 ataagtctgt tcttcgtcag aatcgagatt cagcaccgtt acggtagcac caaaaataac
    41041 gcgcccattg ttaggcattt tggtgacatc aatcacctgc gcgtttgaca gcttggcttc
    41101 gatgtctttg atacggcctt cgcagaagcc ctgctgttca cgagctgcgt ggtattcggc
    41161 gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga tttcaggacg
    41221 gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tctgcgccgc gtaaggtcat
    41281 cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag tcttcaccca
    41341 ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca aaaaaatacc
    41401 gacccgggta caagtcccag gtcagctaca attcacattt tgatagtcat tttaccctga
    41461 agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg acggctcgat
    41521 tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat tgaccagctg
    41581 tatagcgttc agtgttcagg ccgcaaatgt tgatgagtac attactcaac tccccgctgg
    41641 tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta ttgattacca
    41701 cagtcagcag atggcgctgc ctgccagtac ccagaaagtg attactgcgc tggcggcgtt
    41761 gattcaactc ggccccgatt ttcgttttac cacgacgctt gaaaccaaag gcaatgtgga
    41821 aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga cgttaaaacg
    41881 tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc aaatcgatgg
    41941 caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg gctggccatg
    42001 gaatgacatg acacaatgct ttagcgctcc gcctgccgcc gccatagttg accgcaactg
    42061 tttctccgtc tcgctctaca gtgccccaaa gcctggtgat atggctttta tacgcgtggc
    42121 atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgtg gttctgccga
    42181 agcgcaatac tgcgaactgg atgtggtgcc aggcgacctg aaccgcttta cgctgacggg
    42241 atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg atggagccag
    42301 ctatgccggt gcaattctga aagatgagtt aaaacaggcg ggtatcacct ggagcggaac
    42361 actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta aacagtcggc
    42421 cccgctgcac gatctgctta agattatgct gaaaaagtcg gacaacatga tcgccgatac
    42481 ggttttccgc atgataggcc atgcgcgctt caatgtgcct ggaacatggc gggccgggtc
    42541 ggacgccgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa acaccattat
    42601 tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca tgatgcaggt
    42661 gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc tgccgctggc
    42721 gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg tggatggaaa
    42781 agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat tcattaccac
    42841 agcgagcggg caacgaatgg cgtttgtgca atatctttct ggctatgcag tagaacctgc
    42901 ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt ataaagatat
    42961 ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggcttc agattattga
    43021 caaagtgcgc tttgtttatg ccggatgcgg cgtaaacgcc ttatccggcc tacaaaatcg
    43081 tgcaaattca acatattgca attctcttgt aggcctgata agcgtagcgc atcaggctga
    43141 tttggcgttt atcatcagtg attaacgctt gtaaatgaac tcaacgcctt cttcgtcgtc
    43201 ttcgtcccag tcgtcatccc agtcttcatc atcctcttca gcaatctctt caagctgctg
    43261 gcgatgataa tcatcccaca tgaattcgac tttctctggc tgtttcgctt cttcagcctg
    43321 cacgaccggg ttttcaatga taaaggtcat cacatcccag cagagatctt tcacgcccag
    43381 tccactcgcc gcagagatca gataatattt atcttcccag cccagcgcct cagcgatcgc
    43441 tttcgctttc tcttcggctt ctaccttatc cagcagatcg atcttgttga acactaacca
    43501 acgcggtttc gccgccagat cctggctgta tttttccagc tcgctgataa taatacgcgc
    43561 gttttcaacc ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca acaggacgcg
    43621 gcaacgttcc aggtgcttca ggaagcgaat gcccagacct gcgccttccg cagcgccttc
    43681 aatcagtcct ggaatatcgg caacaacgaa gctcttttcg ttgtccattc gtaccacacc
    43741 cagacttggc accagagtgg taaacggata atccgccact ttcggtttag ccgccgatac
    43801 cgcacgaata aaggtcgatt tacccgcgtt tggcatcccc aacataccga cgtcagccag
    43861 cagcatcagc tccagcagca gctcgcgctt atcgcccggc gtgccgttgg ttttctgccg
    43921 cggtgtacgg ttaacggacg atttgaaacg ggtattgccc agaccgtgcc agccgccctt
    43981 agcaaccagc agacgctgac cgtgtttggt catatcgccc atggtttcac cagtaccctg
    44041 gtcgattaca cgcgtaccta ccggcacttt aattgtgacg tctttaccgc gcttaccggt
    44101 acagtcgcgg cttgcgccgt tctgaccgcg ctctgcacgg aaagattttt caaaacgata
    44161 atcgataagc gtgttcaggt tctcgtcggc ttccatccat acgtcaccac catcaccgcc
    44221 gtcgccgcca tccgggccgc ctttcggaat atacttttcg cggcggaagc tcacgcaacc
    44281 attaccgcca tcacctgcaa cgaccagaat cgatgcttca tcaacaaact tcattttatt
    44341 ctccgtaaat cattcgcctg cgcggggttg cgataccacc gttgtatgct tacgtaatcc
    44401 gccccaaata cgatgaccaa tggcggaata catcgcgccc gcaaccacga caaacgcacc
    44461 gagataacct aaaaggttta acatcggtct ggcgaagaaa tcgggccagg ccagtgataa
    44521 aagatctgaa aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc tcacctgcgc
    44581 tgcctgccag cgagccatcg cttccgccag ggcgccatat cctaccaatg tattcagtcc
    44641 gcaaaaaatt aaacatgcga gctgccagtg gctaagctgc gctatcactc caggctttgc
    44701 cagagggaag agcgcaattg tacataaagt gtacagtaaa aacaggatct gcggtgaggc
    44761 cagccgacgc aataaaacct tttgcgccac gccataactc acccaaaccg tcgcagcacc
    44821 gaccccaaag ataactcccc aggtgtaatc ggtgagcttt gtaaatatct cgaccagact
    44881 ggtgttaaaa aacatcacca ggccgctcag gagcatcaat gcccctacaa cctgagtgct
    44941 gcgcattttc tctttcagga taaatacgct ggcaaccatc atgccaactg gcgagagttg
    45001 cccaatcacc tgcgaagcgg tcggactcag gtattgcaag gatgagctga acaggatgaa
    45061 gttcccaaac agcccggcgg tcgccactgc caacaaaatc aaccagcgtg gcttacgaaa
    45121 cacgcgtaat ggcggcaacc tcttcttcac cgcaagaatg gcacccaggc caatactcgc
    45181 catcaagaaa cggtaaaaca cgattgtcgg aggttccatc acctccagca cctgcttcat
    45241 tgcgattggc aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa tgccaatgcc
    45301 tgcctgctgc ttcataccgt attccctgca aagcgcattt gcggattaac gtcgcaccat
    45361 gctcattttt accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg ggctttcatc
    45421 cgttaccggg acgcgaaaaa cttattcagc ttcgatgctg ataaatttac ggtttttcgg
    45481 gcctttaact tcgaatttca ctttaccgtc tgctttagca aacagagtgt ggtcacgacc
    45541 gcaacctacg ttagcgccag cgtggaattt ggtaccacgt tgacgaacga tgatgctacc
    45601 cgccagaacg gattcgccac cgaaacgctt aacgcccaag cgtttagctt ctgaatcgcg
    45661 accgttacgt gtggagccgc cagccttttt atgtgccatt tgaaatctct cctcaggtct
    45721 taggcgctga tgccagtaat tttcacatca gtgaaccact gacgatggcc ctgctgctta
    45781 cgatagtgtt tacgacgacg aaacttaacg attttaactt tctcgccacg accgtgagca
    45841 acaacttcag ctttgattac gccgccatca acgaaaggaa cgccgatttt gacttcttca
    45901 ccgtttgcga tcatcagcac ttcagcgaac tcaacagttt cgccagttgc gatgtccagc
    45961 ttttccaggc gaacggtctg accttcgctt actcggtgtt gtttaccacc actttggaaa
    46021 accgcgtaca tataaaactc cgcttccgcg cacacctttt caatgattca gagtgcgcta
    46081 taaatattca caatagggcg cgaatattac gcaaaacgca cgcctttgac aagtgctaca
    46141 gtcaatacac gaagaaaaaa aacacaactt gtacggtaac gtttatctgt gccatttttt
    46201 cagtacaatc accctatatt cctaaccata aaccctaagt tgcctttgtt cacagtaagg
    46261 taatcggggc gaaaagcccg gcttttgcga tgaatttaga aaaaatcaat gagttaaccg
    46321 cgcaagatat ggcgggtgtt aatgcggcaa tccttgagca gcttaattcc gacgtccaac
    46381 tgatcaatca gttaggctat tacatcgtca gcggcggcgg taaacgtatt cgtccgatga
    46441 ttgctgtact ggctgcacga gctgttggct atgagggaaa tgcgcatgtc accattgctg
    46501 ccctgatcga gtttatccac acggcgactc tgctgcacga cgacgttgtg gatgaatcag
    46561 atatgcgcag gggtaaagct accgccaacg ccgcatttgg caatgccgcc agcgtgctgg
    46621 taggcgattt tatttatacc cgcgctttcc agatgatgac cagcctcggt tcactcaaag
    46681 tgctggaagt catgtcagaa gccgtaaacg tcatcgcaga aggtgaagtt ctgcaactga
    46741 tgaatgttaa cgatccggac atcactgaag aaaactacat gcgcgttatc tatagcaaaa
    46801 ccgcgcgtct gtttgaggct gccgcgcagt gttccggaat tctggctggc tgtacgccgg
    46861 aggaggagaa aggcctgcag gattatgggc gctatctcgg cactgctttc cagttgatcg
    46921 acgatttact cgattacaat gccgatggcg aacagttagg taaaaacgtc ggcgacgatc
    46981 tgaacgaagg taaaccgacg ctgccgctgc tgcatgcgat gcatcatggc acaccagaac
    47041 aggcacagat gatccgtacc gccatcgaac agggtaacgg tcgccatctt ctggaaccgg
    47101 ttctggaagc aatgaacgct tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag
    47161 aagcagacaa agccatcgca gcgttacagg tgctcccgga caccccttgg cgagaagcac
    47221 tcatcggcct cgcgcacatc gctgttcaac gcgatcgtta atcccctccc ctcatcccgc
    47281 gcagtgcgcg ggatgattcc agtaaactcc ataagaatac ttattcagcg taataatgca
    47341 ctgaaaaacc agtgtataca tctttatata ttctgaatat tcacactctt tacaggaact
    47401 ttttagagca atcggccatc aggagtatag tgatgctcga cagaagaagt gttctgaatg
    47461 aaagcgacta acttaaggag tgaggaaaga tggaaagtaa tttcattgac tggcatcccg
    47521 ctgacatcat tgcgggtttg cgcaagaagg gaacgtcaat ggcggcggaa tctcgcagaa
    47581 atggtttgag ttcctcaacg ctggcgaatg cattatcgcg cccatggccg aaaggagaga
    47641 tgattattgc gaaggccctg ggaactgacc cctgggttat ctggccatca cgctaccatg
    47701 atccgcagac ccatgagttt atcgacagaa cgcagttgat gcgtagctac actaaaccga
    47761 aaaaatgagt ggtctggcgg tagccccgcg tacggggctg ccagctttca gacgattact
    47821 cgcctttcac acgctcaata tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt
    47881 agccacgatc gatgtgataa atacgatcaa ccaccgtcgt cccttccgca atacagccag
    47941 ccagcaccag gcttgctgat gcacgcagat cggttgccat aacctgtgcg ccagaaagtt
    48001 tttcaacacc gtgacaaata acggtattgc tttcgatttc ggcgtgcgcg cccatacggc
    48061 tcagctctgg cacatgcata aagcggtttt caaagaccgt ttcggtgatg aatccagtcc
    48121 cttctgccac caggttcaac agcgtgaact gggcctgcat atcggtcggg aatgccggat
    48181 gcggcgcggt acgtacgtta acagccttcg gacgtttgcc atgcatatcc aggctaatcc
    48241 agtcttcgcc gacttcgatg tccgctccag cgtcacgcag tttcgccagc acggcgtcga
    48301 gagtatctgg ctgcgcgtta cggcagataa ttttgccgcg agaaatcgcc gccgccacca
    48361 ggaatgtacc ggtttcgata cgatccggca gcacgcgata gacaccgccg cctaaacgtt
    48421 ccacaccttc gatgacgata cgatcggtgc cctgaccgct aattttcgca cccagcgtaa
    48481 tcaggaagtt cgcggtatcg acgatttccg gttcacgcgc tgcgttttca ataatcgtgg
    48541 taccttccgc cagggttgca gcacacatga tggtcaccgt tgcgccaacg ctgactttat
    48601 ccatcacgat atgtgcgcct ttcagacgac catcgacgga agctttaacg taaccttctt
    48661 ccagtttgat ggtcgcgcct aattgttcga ggccagaaat gtgtagatca accggacgcg
    48721 caccgatcgt acaaccgcca ggcagtgaaa cttgcccctg accaaagcgc gctaccagcg
    48781 gccccagtgc ccagatagaa gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata
    48841 cattaacgtc gcgggcatca atatgcacag aaccattacg ttctactttc gcacccagct
    48901 ggcttagcag cttcattgat gtatcgacgt ctttcagttt cgggacgttc tggatctcta
    48961 ccggttcttc cgccagtagt gcggcaaaaa ggataggcag agcagcattt ttagcgccgg
    49021 aaattgtgac ttcgccctgg agcttcgttg gcccctgaac acgaaattta tccatttagt
    49081 ttgttctcag ttaacaattc atatccgcta ccggcgaatc gcccatagct caaaagccgt
    49141 tcagtttgcg atcgcgcgcc cactccgcag gggtatacgc tttaatcgac acagcatgaa
    49201 tgcggttatc cgcaatatat tccatcagcg ggccatagac cgtctgctgt tttttaaccc
    49261 gactcatgcc gtcaaacaac tcacccacgg caataacctg aaagtggctg ccatcgccgg
    49321 aaacgtggac ttcctggagg gagagagcgt tcatcaacac gctctgaatt tcattatttt
    49381 ccatgggatc ttcaatcatc agttaataaa ccagcgaaac atcttagagc aaagttgcgc
    49441 tggcataaat aagcaaaaag cctcgctgat aaatcagaca aggctcgact tgcaggcagg
    49501 tttgccggac aggcggttaa cgccatatcc ggcctgaaaa aatttaacga ggcagaacat
    49561 cagcaggcaa attatacaat ttcgccaggg tatacacttt gtcgtttacc ccctgaagcg
    49621 tcacattgtt gccctgcttt ttcgccagat cgataagatg gagcagcagt gccagtcccc
    49681 ccgtatccac gcgggagaca cggctaagat cgatgcaggt aatccccttc accgcttcct
    49741 cacgcatttc ccaaagcggt agcaaaacgt cctgatccag ctctccggat aacgccagcg
    49801 tgtcacccgt ctgcatccag ctcagtgact cgctcattat tttttctctt ccagagtgat
    49861 tttctgttga gaaatcgatt tcagttgcgc agtcaggccg tcgatacctt tggtacgcag
    49921 cagcgttccc cactcgtttt gtttggtggt gatcatactg acgccttcag caatcatgtc
    49981 gtaagcctgc caattgcccg tctgggagtt tttacgccac tggaagtcca gacgcaccgg
    50041 cggacggcca ttcgggtcaa taatggtaac gcgaataggc acaatggttt tatcgcccag
    50101 cggctgttct ggcgcaatct gataggtttg accgtgatac atcgccagcg cctgaccgta
    50161 agcctgcttc aggtactcac ggaaagcggc aaagtaggct tcacgttgag caggggtcgc
    50221 actcttgtaa tactggccca gcaccagcgc accggcgtat ttcacctgta cgtatggcag
    50281 cagttcctga tcaacaatgg tacgcagata atccgggttg gcccgaattt gcggttgctc
    50341 attcttcagg cgatcgaacg ttttctgcgc cgcctcgtcc atcagcttat acggattggt
    50401 ctggtctgcc gcggttgccg cactcagagg tgcaatcacc agcaaagcga ccatcattaa
    50461 acgtttaaac atgcgtcggt tctcctgaaa ttatttcgtt gtacccacag gttcagtggt
    50521 ttcattatta cctggcgcag cagctggcgc atcgccacta ttcttattgt catcgccttt
    50581 actaccgtaa aggaactgac caatgagatc ttccagcacc atcgcagact tagtgtcctg
    50641 aattgtatcg ccatccttca ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt
    50701 taatgccaga tattgttccc ccagcaggcc ggaagtacga atgctcagcg aactggtatc
    50761 tggaatgtgg ttataacgtt gttcaatttc cagcgttacg cgcggcagat aggttttcgg
    50821 gtccagcgta atatccgcca cccgacccac aacaacgcca ccaatactga ccggagagcg
    50881 cgctttcagg ccgccaatgt tatcgaacgt cgcataaagc gtgtaggtcg gttcagtacg
    50941 tatggacgtc acgttcgccg ccttcaggca aacaaacagc gccgccagca gtgctgctaa
    51001 taaaaagata cccacccaaa tttcattttt tttcgtttgc atgaactcaa ttcccaaaca
    51061 tcaatgcggt cagcacaaaa tccagcccca gaacagccag agacgagtgg acaacggtgc
    51121 gagtggttgc ccggctaatc ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg
    51181 aaatccacgt caccgtgatg gcgaacacaa cgctcttaat cagacagttg accagatcca
    51241 tacgccagtc gacggcattt tgcattgccg accagaagaa cccgctatca atgcctttcc
    51301 aactgacgcc gaccagagat ccgccccaga tccccacggc gacgaaaata accgtcaaca
    51361 gtggtaatga aataacccca gcccagaaac ggggagaaat aacccgacgc agcggatcca
    51421 ccgccatcat ctccatactg gagagttgct ctgtagcgcg catcaggccg atttctgcgg
    51481 ttagcgccga accagcacgc ccggcaaaca acaacgcggc aaccaccggc cccagttcac
    51541 gcagtagcga taacgccacc agcataccca gactggtttc cgcactataa gtggtcagaa
    51601 ccagataacc ttgcagcccc aacaccattc cgatgaacac gccagaaaca acaataatca
    51661 gcatcgacag gacgccgaca ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa
    51721 attccggttt gccgaccagc gcattgaata acattaaccc ggcccgcccg aacgttctca
    51781 gggttttaat ccctttatgt ccgagcgacg ccagcgcatt taacagcatg agtggcttaa
    51841 ctccctggta aaagatcagc gtgataatcg ccggcaggat agcggaacgg aacaggcccg
    51901 tcagctatcc cgtccagaaa ctgacgtacg cgcggatcag gattcgcctg caacgcctgg
    51961 gcactgccat gagcgacaat ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt
    52021 aacacttccg gcacatcgtg agaaaccacc acacaagtca cgcccagcgc gctgttcagc
    52081 tcagaaatca gcttcaccag tacgcccatg gtgatgggat cttgcccaac aaaaggttca
    52141 tcaaacatga tgagatccgg ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc
    52201 atcccaccgg aaagttcaga aggcattagt ttagccgctc cacgcagccc cacggcctcc
    52261 agcttcatca tcaccgtact atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt
    52321 ggataggcga cgttgtcaaa tacgttcata tcagtgaaca acgccccgga ctgaaataac
    52381 atgctcatcc gtttgcgcac tgtatacagg cgcgaacgag acatcgccgg aatattctca
    52441 ccatcgaaaa ggatctcacc atgatctggt gcgatttgcc cgccaatcag acggagtagc
    52501 gtcgttttac cgatgcccga tggccccatg atcgccgtga tcttccctcg cggcacggtc
    52561 agggaaatat tatcgaagat gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg
    52621 actaaattcg ccacagactg ctccataatt cacccttcgt cttgcgttga ttttctaagc
    52681 atggcgctca atttaacctt gaacccaaca tatttacaga atattacccg ccgtggttag
    52741 cgaaagctgg catttgtttt actttttagc cgcataaagt caaaattaag catccgttac
    52801 ggctttctga aaatcttcag cggaccggcg agtatacctg aagaaaggac gttagatgct
    52861 tttagctacg gcactgttaa ttgttggttt acttttggtc gtttacagtg ccgaccgcct
    52921 ggtttttgcc gcgtctattc tttgccgaac ctttggcatc ccgccgctga tcatcggcat
    52981 gacggtggtc agtattggta catcgttacc agaagtcatc gtctcgcttg ccgcgtctct
    53041 gcacgaacaa cgcgatttag ccgttggtac agccctcggc tcaaacatta tcaatatatt
    53101 gctgatcctc ggtctggctg cgctggttcg tccttttacc gtccattctg atgttctacg
    53161 ccgtgaatta cccttaatgt tgttggtcag cgtggtggcc ggttccgtac tctatgacgg
    53221 acaacttagt cgcagcgatg gtatctttct cctctttctg gctgtgctat ggctgctgtt
    53281 cattgttaaa cttgcacgtc aggctgaacg tcaggggact gacagcctga ccagagagca
    53341 gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc tcggcattgc
    53401 gcttatcatc atgccagtgg ccacgcggat ggtggttgat aacgccacgg tgctggcgaa
    53461 ttactttgcc atcagcgagt tgacgatggg tctgacggca attgctatcg gaaccagcct
    53521 gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca ttgctgtcgg
    53581 aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat
    53641 aacgccagga gagattgatc cactggcgta cagtcgtgac tacagcgtga tgttgctggt
    53701 gagcattatt tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc gtggtgtagg
    53761 ggtattatta actggcggat ttatcgtatg gctggcgatg ttgtactggt tatcgccaat
    53821 actcgttgaa taactggaaa cgcattatgt cacacgtaga gttacaaccg ggttttgact
    53881 ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg gagcttgatc
    53941 aatacatcaa tcagaatttc acgcttgcct gtgaaaagat gttctggtgt aaagggaaag
    54001 ttgtcgtcat ggggatggga aaatcggggc atattgggcg aaaaatggcg gcaacgtttg
    54061 ccagcaccgg tacaccttca tttttcgtcc atcctggtga agccgcgcat ggtgatttag
    54121 gcatggttac cccacaggat gtggtgattg ctatctctaa ctctggtgaa tccagcgaaa
    54181 tcacggcctt aattccagtg cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc
    54241 gcccggagag cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag
    54301 aagcctgtcc gttagggctg gcaccgacca gcagcaccac cgccacgctg gttatgggcg
    54361 atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat tttgcgctct
    54421 cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata
    54481 cgggcgatga gatcccgcat gttaagaaaa cggccagtct gcgtgacgca ttgctggaag
    54541 ttacccgcaa aaatcttggt atgactgtca tttgcgatga caatatgatg attgaaggca
    54601 tctttaccga cggtgattta cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa
    54661 gtattgccga tgtgatgacg ccggggggaa tacgtgtgcg ccctggcatt ctggccgttg
    54721 aggcactgaa cttaatgcag tcccgccata tcacctccgt gatggttgcc gatggcgacc
    54781 atttactcgg tgtgttacat atgcatgatt tactgcgtgc aggcgtagtg taaagattca
    54841 aggataaaca acaatgagca aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag
    54901 cgccgacgtt atggcaaaag cagagaacat tcgtctgctg atcctcgacg ttgatggcgt
    54961 actgtcagat ggcctgattt atatgggcaa taacggcgaa gagctgaaag cgttcaatgt
    55021 tcgtgacggt tatggcattc gttgtgcgct cacctctgat attgaagtcg ctatcattac
    55081 cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca ttggggatca ctcacttgta
    55141 tcaggggcag tcaaacaaac tgatcgcctt tagcgatctg ctggaaaaac tggcgattgc
    55201 cccggaaaat gtggcttatg tcggcgatga tctcatcgac tggccggtaa tggaaaaagt
    55261 gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg ccgattacgt
    55321 gacgcgcatt gctggcggtc gtggcgcagt gcgcgaagtt tgcgacttat tactcctggc
    55381 gcagggcaaa ctggatgaag ccaaagggca atcgatatga gtaaagccag acgttgggtt
    55441 atcattgtgc tatcactggc ggttctggtg atgatcggca ttaatatggc cgaaaaagac
    55501 gataccgccc aggtggtcgt caacaacaat gatcccacct ataaaagcga gcatacggac
    55561 acgctcgtct ataacccaga aggggcacta agctatcgat tgattgctca acacgttgaa
    55621 tattattccg atcaggccgt ttcgtggttt acgcagccgg tacttaccac gtttgataag
    55681 gataaaatcc cgacatggtc cgtaaaagca gataaagcca agctgaccaa tgaccggatg
    55741 ctctatttat atggacacgt tgaagtcaac gcacttgtgc cggactctca acttcgcaga
    55801 atcacgacgg ataacgcgca gatcaatctg gtgacgcagg atgttacctc tgaagacctc
    55861 gtcacgttat acggaacaac atttaactcc agcggtctga aaatgcgcgg caacttacgc
    55921 agcaagaacg ccgagctgat tgaaaaggtt agaacatcct atgaaattca aaacaaacaa
    55981 actcagcctt aatcttgtgc ttgccagctc acttctggcc gccagcattc cggcatttgc
    56041 cgtaaccgga gacactgatc agccgatcca cattgaatcg gaccagcaat ctcttgatat
    56101 gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc acccagggca ccatcaaaat
    56161 taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag aagtgattga
    56221 cggctacggt aaaccggcaa cgttctacca gatgcaggac aacggtaaac ccgttgaagg
    56281 tcacgcttcc cagatgcact acgaactggc aaaagatttt gtcgttctga cgggtaatgc
    56341 ttatctgcag caggtcgata gcaacattaa gggcgataag atcacttacc tggtgaaaga
    56401 gcagaaaatg caggctttca gcgacaaagg caagcgcgta accaccgttc tggtgccgtc
    56461 gcagctgcag gataaaaaca acaaaggcca gaccccggca cagaagaagg gtaattaatt
    56521 cgttatggca acattaactg caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt
    56581 agaagacgtc agcctgaccg tcaactccgg ggaaatcgtc ggtctgctgg ggccaaacgg
    56641 tgccggtaag accaccactt tctacatggt tgtaggcatt gtgccgcgcg atgcgggcaa
    56701 catcattatt gatgatgacg atatcagtct gctgccactg catgcacgcg cgcgccgcgg
    56761 tatcggctat ctgccacagg aagcctccat tttccgtcgc ctcagcgttt acgataacct
    56821 gatggcggta ctgcaaattc gtgacgactt gtctgctgaa caacgtgaag accgcgcgaa
    56881 cgagctgatg gaagagtttc acattgagca cctgcgtgac agcatggggc agtcactctc
    56941 cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat
    57001 tctgctcgac gaaccgtttg ccggggttga cccgatctcg gttatcgaca ttaaacgcat
    57061 cattgagcac ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca acgtgcgtga
    57121 aacactggcg gtttgtgaac gcgcttatat cgtcagtcag gggcatttga tcgcccacgg
    57181 cacgcctaca gaaatcttac aagacgaaca cgttaagcgt gtataccttg gggaagactt
    57241 cagactctga tagggtagaa gtttgcgacg ttttagcagg agagtacgat tctgaacatg
    57301 aagcaaggtt tgcaactcag gcttagccaa caactggcga tgacgccaca gctccaacag
    57361 gcaattcgtc tgttgcagtt gtcgacgctg gaacttcagc aggagctaca gcaggcgctg
    57421 gagagtaatc cgctgcttga gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg
    57481 caagacagtg aaacgctgga caccgccgac gcgctcgaac aaaaagagat gccggaagag
    57541 ctgccgctcg atgccagttg ggacaccatt tacaccgctg gtacaccatc cggcaccagc
    57601 ggtgactaca ttgacgatga gctgccggtc tatcagggcg aaacgacgca gaccttgcag
    57661 gattacctga tgtggcaggt cgagctgaca ccgttttccg acactgaccg cgctattgct
    57721 acctctatcg tcgatgccgt tgatgacacc ggttatctga ctgtcccgct ggaagatatt
    57781 ctcgaaagta tgggcgatga agagatcgac atcgacgagg ttgaagccgt ccttaagcgg
    57841 atccaacggt ttgatccggt cggtgtagcg gcaaaagatc tgcgtgactg cctgctaatc
    57901 caactctccc aattcgataa aaccacgccg tggctggaag aggccagatt gatcattagc
    57961 gatcatctcg atctgttagc caatcacgac ttccgcactt taatgcgcgt cacgcgtctg
    58021 aaagaagatg tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc gcgccccggg
    58081 cagtcgatcc agactggcga acctgagtat gtcattccag atgtgctggt gcgtaagcat
    58141 aacggtcact ggacagtaga actcaacagt gacagcattc cgcgtctgca aatcaaccag
    58201 cactacgcct cgatgtgcaa taacgctcgc aatgatggtg acagccagtt tatccgcagc
    58261 aatctgcagg atgccaaatg gttgatcaag agtctggaaa gccgtaacga tacgctactg
    58321 cgcgtgagtc gctgtatcgt tgaacagcag caagccttct ttgagcaagg cgaagaatat
    58381 atgaaaccga tggtactggc cgatatcgcc caggctgtcg aaatgcatga atcgacgata
    58441 tctcgcgtga ccacgcaaaa atacctgcat agtccacgag gcatttttga actgaagtat
    58501 ttcttttcca gtcacgtcaa taccgagggc ggcggcgaag cttcctccac ggcgattcgt
    58561 gcgctggtga agaaattaat cgcggcggaa aacccagcga aaccgttgag cgacagcaag
    58621 ttaacctctt tgctgtcgga acaaggtatc atggtggcac gccgcactgt tgcgaagtac
    58681 cgagagtctt tatccattcc gccgtcaaac cagcgtaaac aactcgtttg acccaaccga
    58741 taaggaagac actatgcagc tcaacattac cggaaataac gtcgagatca ccgaggcact
    58801 gcgcgaattt gttacagcca aatttgccaa acttgagcaa tattttgacc gaatcaacca
    58861 ggtctatgtt gttctgaaag tggagaaagt cacccacacc tcagatgcaa cactgcatgt
    58921 aaacggtggt gaaattcatg ccagcgcgga aggtcaggac atgtacgctg ccattgatgg
    58981 cttaattgat aagctggcac gccagttgac caaacataaa gataaactga aacaacacta
    59041 attgtccggg caattagcat gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt
    59101 acggttaatg ctccgagcct gttccactgt ttgagtgggc aggttcttag gtgaaattat
    59161 gacaaataat gatacaactc tacagcttag cagtgttctt aacagggaat gtacgcgaag
    59221 ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa
    59281 acaacttagc ctgccgcctc aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg
    59341 cagtaccggt atcggcaatg gtattgcaat tccgcatggc aaactggaag aagatactct
    59401 gcgtgccgtt ggcgttttcg ttcagctcga aacgcctata gctttcgatg ccatcgacaa
    59461 ccaaccggtg gacttacttt ttgccctgct ggtgccggca gaccaaacta aaacgcacct
    59521 gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc gccgtttacg
    59581 cgcagcccag agcgatgaag agctgtatca aatcattacg gataccgaag gtactccgga
    59641 tgaagcgtag ttattcggta atgtctcttt tagacgttgt gaggagaaac agtacatggt
    59701 actgatgatc gtcagcggac gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga
    59761 agatatgggt ttttactgcg tggataacct tcccgtagtg ttgttacccg atctggctcg
    59821 aaccctggcc gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta atatgccgga
    59881 gtcaccagaa atattcgaac aggcgatgag taacctgcct gacgctttct caccgcaact
    59941 actgttcctg gatgccgacc gtaatacctt aattcgtcgt tacagtgaca cgcgccgact
    60001 gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag aaagcgatct
    60061 gctggagcct ctgcgttcgc gagcggatct gattgtcgat acctcagaaa tgtccgttca
    60121 cgagctggct gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg aactgacaat
    60181 ggtctttgag tctttcggct tcaaacacgg tatccctatc gatgcagatt acgtctttga
    60241 cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg cgtccaatga caggtcttga
    60301 taagcctgtc gccgcgttcc tcgaccgcca cacagaagta cacaatttta tctaccagac
    60361 gcgaagctat cttgagctat ggttacctat gctggaaacc aacaaccgta gctacctgac
    60421 ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg tatattgcag agcaactggc
    60481 tgactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc tggaaaaacg
    60541 taaaccatga ccgtcaagca aactgttgaa atcacaaaca agctgggcat gcatgcccgg
    60601 cctgcaatga agctgtttga attaatgcag ggttttgacg ctgaagtgct gttacgtaat
    60661 gacgaaggca ccgaggctga agccaacagc gttattgcgc tgctgatgtt ggattctgcc
    60721 aaaggacggc agattgaagt tgaagcgacc ggtccacaag aagaggaagc actggccgcc
    60781 gttatcgccc tctttaactc tggttttgat gaagattaat cttcatcact ttgacattca
    60841 aatcccttca agctcccccg gcaataagaa aagcgcgtac tgaaatcgtt ctcaatcaac
    60901 gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa accttaagaa
    60961 gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag aaaatatttc
    61021 caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga agacatcgga
    61081 aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac catcgtggcg
    61141 atggtggcga taaagagatc cgccgtgagt taaagtatta cgcgcatctt ggtcgtcggt
    61201 taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat tgcggaaccg
    61261 gttatgtcta cgatgtgatt gccgatttcg acggcaagcc ctccattacg ctgaccgaat
    61321 ttgctgaaca atgccgttac gaagaagacg ttgcacaact gcgccagtta ctgaaacagc
    61381 tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg cagaatatcc
    61441 tctgtcatcg catcagtgag tcagaggtta tccccgtcgt ctgcgacaac attggtgaaa
    61501 gcacattgat tccactggca acctggtcga agtggtgttg tctgcgtaag caagagcggt
    61561 tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa gatctgcaac
    61621 ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc tggtgttgtt
    61681 gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc tgacggctat
    61741 gcacgtagcc cgatggcgcg gagactttga aacgacgtgg attaggtaat acagctgcca
    61801 gtaatgcagc ttccgatcgg gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg
    61861 cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa accgtcagga
    61921 tacgcttttt gctccagacc gtttctatcc ccagcgttaa tccagcttcc agcccttttc
    61981 gtacccagct acgcccatcc cataaaaaga gatttttggc tgtctgctga gaaatcgttg
    62041 atgcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc tcaatggaag
    62101 cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc tgcggcaatc accgccagtc
    62161 ccatccatgg cgagatttga tccatactga cccagtcaga atgtgccacg taacgaaaat
    62221 tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag ggaacaggcg
    62281 caacgctaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc attaaccgca
    62341 aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt cagccagaac
    62401 cagcacgcgg gataccagct tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag
    62461 catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat catcgacagg
    62521 acagggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga tatcagtacc
    62581 gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg caatacacat
    62641 aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta attcacggtc
    62701 aacggtgcat tcgctaccaa gactggcaaa attgcttaaa ttcttcgccg cgccaaaccc
    62761 ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag gacggatttc
    62821 accgcgcgtt attcgtgccg cttcaatcag cacattgcgc gtttccgtca tcgcctcgcc
    62881 agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga ccgcctgcgc
    62941 accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag aaccgtcata
    63001 gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc tttcgcaatt
    63061 gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt aactgattca
    63121 tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa acagtgctgt
    63181 gcccttgtaa ctcatcataa taatttacgg cgcagccaag ttttccctgg tgttggcgca
    63241 gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc acccacgctt
    63301 tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat tcaccgacgt
    63361 tatcttccca ggccggaagg tcaggagact gaatttgctg acccagctgt tgcagatggc
    63421 gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca atgccttttt
    63481 tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc atcttctcaa
    63541 acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac tgttccagca
    63601 tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc gtactctcct
    63661 catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga acagaaagcg
    63721 gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct ttcagcacgt
    63781 tagcggttaa ggccaccagc ggcggtaaat cctcgcgcgg ataacgtttc gtcagttcac
    63841 gagagatatc cagcccggtc atatctggca actgaatatc cagcaacacc aggtcgtatt
    63901 cgcccggttt aaacatctcc agcgccgcct tgccggtcat ggcgacatca acgctgttac
    63961 ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag ttcaatgtct tccaccagca
    64021 gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg
    64081 ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt tcgctggtaa
    64141 ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga ccaataccgg
    64201 tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg gcgaaaattt
    64261 tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa tgcagcatat
    64321 cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt
    64381 tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg acctgatgcg
    64441 gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg
    64501 agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta tcaagctgga
    64561 ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc cccagcgtga
    64621 cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt tcggtatcca
    64681 gcagaatgcg gctcagaccg acgataccgt tcagcggtgt acgcaattcg tgactgatgg
    64741 tggagataaa cgtcgttttg tcgcggctgg cccgttcaag cgcatcctga taccgcttac
    64801 gctcggtaat gtcgcgacca aagcccatca aaccgtgacg tttacccacg cggtcgtagt
    64861 acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc agccactgtt
    64921 cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg acttttgcgg
    64981 cggcttccgg tgagtaaaca tcagcaggtt tcaggtgaac cagttgtttt tcgctttttc
    65041 cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg ttacgataaa
    65101 aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc tgctcgagct
    65161 gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag gtttcctgca
    65221 gttctgcttc cgctttttca cgaacggcaa tttcctgatt tagctgggca atattatctt
    65281 ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc
    65341 gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga aagtagaccg
    65401 cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat aacatcaatg ctttcgacct
    65461 gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg acgagggcca
    65521 gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca acataatact
    65581 gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg ccccaataat
    65641 acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc cctcaacgac
    65701 ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc catgttcatt
    65761 cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca gcggagcaag
    65821 cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg gtggcgtatg
    65881 gcgaatcatt tctccggcag tgagcgtgta atcctccagt tcaataccgt tcaaacgccc
    65941 cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcagcggat gcagctttat
    66001 gccgtccacg ccggtttcaa ccactcgctc cagcgtttgc aaacattcag cctgcccttc
    66061 gccaggcagg ccgacaatta agtgggaaca caccttcagc ccgcgctgac gtgtcagctg
    66121 ggttgtacgc tgataacagg caaaatcatg gccgcggttg atgcgatgca gtgttttgtc
    66181 gtgggcggtt tgtagcccca gctccagcca cacttcgtaa ccctggtcct tatattcgca
    66241 aagcagatcc agcaccgcat ccggcacgca gtccgggcgg gtaccaacac acaaaccgac
    66301 aatattggcc tggctcaccg cctgctgata catagaacgc agcacctgaa cttccgcaaa
    66361 ggtgctggta tacgcctgaa agtaggccag atagcgttta gcgcggttaa ctaaattcgc
    66421 ctggtgcgcc agttgctcgg caatggaacg atgctgctgc gcttcatcgg caaacgaggc
    66481 aacattacag aatgtgcagc caccacgccc gatggtaccg tcacggttag ggcagctaaa
    66541 accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga gatcaccacc
    66601 aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt gaaaagaggc
    66661 caaagcctgc catttttagc tcaattcggc gatgacctgg atcaatcgtc ccggcccgct
    66721 tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca cataaatcac
    66781 ttttgcttat tttgtgtcag atttttttat ctccttatgg attttaggca aaaacagtag
    66841 catgaaacgt cattaccaat taaggcagta taaaatgctg gttttctcgt cagttcaagg
    66901 caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct ctaacgactt
    66961 cccttttagc cttaaagata aaatctattt taatttcagc cgtttaataa agaattttgc
    67021 gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc tttcccgata
    67081 agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat ttatgcagta
    67141 attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg gggcgaatgc
    67201 gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct ttctgtgccc gtgcgcagcg
    67261 gttcggtagt ggggttcccg cagagcctgg gggaggttca cgatatgttg tacgataaat
    67321 cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc gaacctagcc
    67381 acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt ggcgcgattc
    67441 tcgccgatgg taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg gatcgctttt
    67501 ttcgcatcgt tgcgcaggag cgcggctggc gtttagcgaa aaactacgcc gtcgggatgc
    67561 tattcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt gaagaagaac
    67621 tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac gaaggcgtgc
    67681 tgggtgaaat cgccctctcc tctctgccac gcattgagca aatttttgtg aacgccccgg
    67741 caggctggcg tccacgcgat atggagcgcc gtctgtttat cgcccgccgc cgcattgaaa
    67801 agcgtctcga agccgacaaa gacttctacg tctgtagcct gtcgaatctg gtgaacatct
    67861 ataaaggtct gtgtatgccg gcggatttgc cgcgcttcta cctggacctc gcggacctgc
    67921 gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc gtaccgcgct
    67981 ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac accatcaccg
    68041 gtaaccgcca atgggcgcgt gcgcgtactt ataaattcca gacaccgctt atccctgacc
    68101 tgcacgacgc cgcaccgttc gtcaacgaaa ccggctctga ctccagttcg atggataaca
    68161 tgctggaact gctgctggca ggcgggatgg atatcatccg cgccatgcgt ctattagtac
    68221 cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc tttgacttta
    68281 actccatgca tatggagccg tgggatggtc cggcgggcat cgtgatgtcc gatggtcgct
    68341 tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc acgctacgtc attaccaaag
    68401 acaagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct gacgaagtgg
    68461 tcgaaaaagg ccgcgtcggg ccaggcgaat tgatggtgat cgacactcgc agtgggcgta
    68521 ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccacccttat aaagagtgga
    68581 tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa gaagtgggta
    68641 gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac tacagcgcgg
    68701 aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg gtcggttcga
    68761 tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt tacgactact
    68821 tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt gaagcgcatg
    68881 ttatgtcgct cgccaccagc attggtcgtg aaatgaacgt cttctgtgaa gcagagggcc
    68941 aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctcggatttc aaacagctca
    69001 cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt gacgtcacta
    69061 aaaccacgct cgaagcgacg gtcaaagagc tgtgcgacaa agcagaacag atggtgcgta
    69121 gcggcaccgt gttgctggtg ctctccgacc ggaatatcgc gaaagatcgc ctgccggttc
    69181 cggccccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc ctgcgttgcg
    69241 atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc gccgtgttgc
    69301 tgggtttcgg cgcgacggct atttatccgt accttgccta tgaaacgctg ggccgcctgg
    69361 tagacaccca cgcgattgcc aaagattatc gtaccgtgat gctcaactac cgtaacggca
    69421 tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc gcctcttacc
    69481 gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc ctgtgcttcc
    69541 agggggcggt cagtcgcatt ggtggggcga gctttgaaga cttccagcag gatctgctga
    69601 atctctcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc ctgctgaaat
    69661 acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg ctgcaacagg
    69721 cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt aatgagcgtc
    69781 cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg gtcaacattg
    69841 ctgatgttga accggcaagc gaactgttta aacgcttcga taccgctgcg atgtctatcg
    69901 gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaacagc atcggcggta
    69961 attcgaactc cggtgaaggc ggcgaagacc cggcgcgcta cggcaccaac aaagtgtcgc
    70021 gcatcaagca ggtggcttcc ggtcgctttg gggttactcc ggcgtatctg gtcaatgccg
    70081 acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt cagttgccgg
    70141 gcgacaaagt cacgccttac atcgccaaac tgcgctattc ggtgccgggc gtgacgctga
    70201 tctccccgcc gccgcaccac gatatctact ctatcgaaga cttagcgcag ctcattttcg
    70261 acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtttcc gaaccgggcg
    70321 ttggcaccat cgccaccggt gtggcgaaag cttatgcgga cttgatcacc atcgcaggct
    70381 atgacggcgg caccggcgca agtccgcttt catcggtgaa atacgcaggc tgtccgtggg
    70441 agctgggact tgttgaaaca caacaggcgc tggttgccaa cggcttgcgt cataaaatcc
    70501 gtttgcaggt cgatggcggc ctgaaaaccg gcgttgatat catcaaagcg gcgattctcg
    70561 gcgcagaaag cttcggcttc ggcactggcc cgatggtggc gctcggttgt aaatatctgc
    70621 gcatttgcca cctgaacaac tgcgccacgg gtgtagcaac tcaggatgac aaactgcgta
    70681 agaatcacta tcacggcctg ccattcaagg tgacgaatta ctttgagttt atcgcccgtg
    70741 aaacccgcga gctgatggca cagcttggcg taacacgtct ggtggatctg attggtcgca
    70801 ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg gcgctgtcga
    70861 agctgctgga gactgccgag ccgcatgcag gtaaggcgct ctactgcacc gaaaacaacc
    70921 cgccgtttga taacggcctg ctgaacgcgc agttgctgca acaggcgaaa ccgtttgtcg
    70981 atgaacgcca gagcaaaacc ttctggttcg atattcgcaa caccgaccgt tctgtcggcg
    71041 cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca gccgatccta
    71101 tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtgtggaac gcgggcggcg
    71161 tggaactgta cctgaccggt gatgccaacg actatgtcgg taaaggcatg gcgggcggct
    71221 taatcgccat tcgtcctccg gttggttccg ccttccgcag ccatgaagca agtattatcg
    71281 gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc cgcgcgggtg
    71341 aacgtttcgg cgtgcgtaac tccggtgcta tcaccgtggt agaaggcatt ggcgacaacg
    71401 gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc gttaacttcg
    71461 gcgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggcgat ttccgcaaac
    71521 gcgttaacac ggaactggtc gaggtcttaa gcgttgacga tctggcgatc catgaagagc
    71581 atctgcgcgg tcttatcacc gagcatgttc agcataccgg ttctcagcgc ggtgaagaga
    71641 ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg aagtccagtg
    71701 atgtaaaagc actgctgggt caccgtagtc gtagcgcagc tgagttgcgc gtgcaggcgc
    71761 agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg cagcgcgttg
    71821 atccgccaaa gaaaccgctg aagatccgca aaattgagtt tgttgaaatt tacgagccgt
    71881 tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc aacccatact
    71941 gcgagtggaa atgcccggta cacaactaca tcccgaactg gctgaagctc gccaacgagg
    72001 ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa caccctgccg gaagtttgcg
    72061 gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg caccctgaac gatgagtttg
    72121 gcgcggtgac catcggcaac attgagcgct atatcaacga taaagcgttc gagatgggct
    72181 ggcgtccgga tatgtccggc gtgaaacaga ccggtaaaaa agtggcgatt atcggcgcag
    72241 gtccggcagg tctggcgtgt gcagatgtcc tgacgcgcaa cggcgtaaaa gcggtcgtct
    72301 tcgaccgtca cccggaaatt ggcggcttgc tgaccttcgg tattccggct ttcaagctgg
    72361 aaaaagaggt aatgacgcgc cgccgtgaaa tcttcaccgg catgggtatt gaattcaaac
    72421 tcaataccga agtgggccgc gacgtacagc tggacgatct gctgagtgat tacgatgccg
    72481 tgttccttgg cgttgggact tatcagtcaa tgcgcggcgg gctggaaaac gaagacgccg
    72541 atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta atgggctttg
    72601 gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg gtccttggcg
    72661 gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg ccagggagcg aagcacgtta
    72721 cctgtgccta tcgtcgtgat gaagagaaca tgccaggttc ccgccgcgaa gtgaaaaacg
    72781 cgcgggaaga aggcgtagag ttcaaattca acgtccagcc gctgggtatt gaagtgaacg
    72841 gtaacggcaa agtcagcggc gtaaaaatgg tgcgtaccga aatgggcgaa ccggacgcca
    72901 aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg gcagatgcgg
    72961 tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa cacagcgtcg
    73021 agctggattc acaaggccgc atcatcgccc cggaaggcag cgacaacgcc ttccagacca
    73081 gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg gtggtgaccg
    73141 ctattgccga aggtcgtaag gcggcagacg gtattatgaa ctggctggaa gtttaagctc
    73201 tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt ctttctggaa
    73261 agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt gttaccggta
    73321 gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag ggacagataa
    73381 tggaatcgct ctcagagaga acctcaacag gctaccagca aatccatgac ggtattattc
    73441 acctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg atgaccgcga
    73501 catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag gccagggctg
    73561 cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg tataagcgtg
    73621 gtttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt caacatgttg
    73681 aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag acaccatctg
    73741 cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga ggctgtttga
    73801 tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca tgcaaataaa
    73861 cgaaggaaag ttgccatgat ttacattgaa cagcttgaat taatccataa aagtggtgat
    73921 gtgttgtatc cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt ccaccttgtt
    73981 ccattcggct taaataagac tcacgacctt ctagaggtgg aagatgcttc agaagcaatt
    74041 cggctggtga ttgatgaacg tcattccatt cgctgcagta cacttacagc aactattacg
    74101 aataaaaagg gcaagcgaat taagcggaca ggtatataca gtatcaaggg tgttaacata
    74161 aaagaataca acgtcaggta atggcacaga gcggtccgtc agttcaggtt tggctctgtg
    74221 ccaaagatgg gtcaggtcac acctgatcta ataaatttaa gccattaaat cagccttaac
    74281 cttcaccacc acctttttaa tctcacccgg ctcgccgaca acgcatcctg gtttatgcgg
    74341 ttctcccggc ataaacacgg cgaacattcc cggttttaag ttgatggttt gctcgttttc
    74401 aatggcgctg caaagctggt aatcatcttc atggtggaac tcttcacatt ggcgcgcagt
    74461 gcctgccatg ccaaacagaa tccgttcctc accgtttaat aacagctgga tatcaatgta
    74521 ttgctcgtgc aattccgctt ttttctcgac gggcgattga gtgttaaacg tcatgacatt
    74581 cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt gcggtctggc
    74641 agctaaggcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag acggtaatga
    74701 ctgtacttca cccatcatca taatttttct ccctgggcca acagcgcagc cccaagtaaa
    74761 cctgcatcat ggcggtagtg cgccgccagt aaatcaacat gaaatgccgc tggctcctgc
    74821 gccagataca tttccaccaa cgccagatac ccttctgcca gaccaacgct gccaccaacc
    74881 accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc aagcgtacgt
    74941 gcggagcgat gaatcagttg ctgcgcctgc tcatcgccct gcccggcgcg tgtgaaaata
    75001 gttctcgcat ccgcgccagc cagctcgccc tgcgctgccg ctgcaatacc gcgaccagaa
    75061 gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg tggatcggca
    75121 agcgtatgcc cgatatgccc cgccagaccg ccagggccgg taagcagttt accgccgctc
    75181 actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatctcggt tacatcgcct
    75241 tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc aatggtcggc
    75301 aaattggtaa gttgttccag cgttttgact aacggaaagt gtagcaatcc accgagatta
    75361 tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc gatggcaacc
    75421 cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg caaggcttct
    75481 ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc gtcagcgcca
    75541 atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtgtggt catagcaccg
    75601 cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta atggcagaac
    75661 cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca ggcgtgttgt
    75721 aacgcccttc ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc agcgccagat
    75781 ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca ataatttcgg
    75841 ctcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc atcgccagta
    75901 aaccgtgatg gtgaatacgt gccagcagcg tttcaacagg caccggacgc gggcggtcgg
    75961 tgccgtcaat ggcgataatg tccgcgcccg cctgcaccag cgcatcaaca tcttcaatat
    76021 aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca ataatcggca
    76081 cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcaatgcga atggcaaccg
    76141 cgcccgcctg ttctgccgct aatgccatgg cggcgacgat ttcgggttta tcgagcgggc
    76201 tgtccggaac cggctggcag gagacaatca ggccaccgtt agcagcgatt ttttgatcca
    76261 gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacat agaccgggca
    76321 acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaacg gcacggcacc
    76381 gctgaatggt ttaccgtcga tagcgtcatg agtacgcaac gcttccgggc gcaaccaacg
    76441 ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg tcagactgaa
    76501 cgagagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg cgccgatgat
    76561 tggggccagt gcgccgccca atgcgccaac gttgtaggta aagcccaggc ccgctgcacg
    76621 ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga tcccttgtcc
    76681 aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc cgccaatcgc
    76741 aaataccgga ataatcagca gctgcgaggc cagcaggcta caaacgtacg ctttgcgggt
    76801 tcccagccag tcaccgagga agccacctac gcagcatccc acggccgcgc caaagccact
    76861 aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt tcagatacgt
    76921 tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga ccaccatcag
    76981 cattacgccc gttggccagc gtttacccgt actttgcacc ataaagctga taaagattgc
    77041 ggcgcataac agcccgagaa cagcgacgat tgcagcattt tgcaggttac cggcgaagca
    77101 gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc gatgttcacc
    77161 acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt tctctttcca
    77221 gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgattg gcaaaatgcc
    77281 gataaagaac agcgcacgcc aaccccagac cggaaccacc aggctataga cctgagcggc
    77341 aacgacggcc cccacagaga agcctgaaat caaaaaacca ctggctttgt tacgcagatg
    77401 ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg ccatccccat
    77461 gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc cgcaggccag
    77521 cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc cgtagcggtc
    77581 acccatagcg ccgagcatca gtccgccgaa ccagcgagag ataaaggctg cggagatcag
    77641 acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga gtaccagggc
    77701 gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg aaaatgcgcg
    77761 ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag tactcattgt
    77821 gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag ggtacagatg
    77881 cgtttatttc ccctcacccg gtaggggcga gcgaggggaa aacaactcac ccgcgctctt
    77941 gcatcaactg ctgggccagc gccttcagtt ctggcaggta tttttcatct accggtccaa
    78001 acggtttgcg gcacagcggc acagaaacga catccatata atgcaggaca gttttcaggc
    78061 cgcggaatac gcccgttttg atcagtaaat caatgacttt gttgcactca gtttgcagtt
    78121 tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga tatcgccagc
    78181 ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag ccggaagcga
    78241 agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg atctgttcca
    78301 tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac gtaaccaggg
    78361 tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg tacaccacca
    78421 tcggcaaacc atccgctgaa tcaataattg ctcgatagtg atcgcagtgt tcttcaaagc
    78481 tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa cgtttagccg
    78541 atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg atgagtttaa
    78601 tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg gaaaggcttt
    78661 gtacaaaggc ttcgccggtc gaaccaccca cgtataaacc gtcgatgccc tgctgaatat
    78721 tgaactgaac caggcggcgc agactcgctt tatccagtgc ttgttgttga tcaaaaggag
    78781 tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct gaagtgatgc
    78841 ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag caacacccca
    78901 tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt tttgttggtg
    78961 gtctgaccga aggcgtgcca ggtagcagag acgctgttga gatgcgattg caacgcacga
    79021 tcggcttcgt caggatcatg acggcggatc gcatcaacga tcgcaatatg ctgttgatag
    79081 ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc ggcgatcagc
    79141 cagtcgagca gggcaacgtg gatcgccatg aagattgggt tacctgggat ctccgccagc
    79201 acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag cgactgactg
    79261 ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcgtg ttcagccgca
    79321 tagcgcacca ggctggattc aaagaacaga cgtaattgtt cgaaatgggc aatcccaccg
    79381 ggatgagaaa ggaaatcttt cgccatgccg gaaagctcac cgatgatagt gtccgcagaa
    79441 ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt gcgttttaac
    79501 gctgccagcg cttcgcgcac agaaggacgc ccgacgttaa agaacgccat cagttcgcgt
    79561 tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg ttccagctct
    79621 tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa gttgcgacca
    79681 attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc cattctgtaa
    79741 ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt aggggaaaat
    79801 ggcaggtttt tctctttgtg cctcatcatt accataatta acggaataat taactattgc
    79861 gaaaaattaa tgtaacgcag ataaacacat tccgattga
//
LOCUS       DAJOSY010000006        78821 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJOSY010000006 DAJOSY010000000
VERSION     DAJOSY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.006"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..957
                     /gene="phoA"
                     /locus_tag="OHN27_000436"
     CDS             <1..957
                     /gene="phoA"
                     /locus_tag="OHN27_000436"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCQ2284943.1"
                     /translation="ILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATS
                     EKCPGNALEKGGRGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREQAQARG
                     YQLVSDAASLNAVTEANQQKPLLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQ
                     RNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAV
                     QRALEFAKKEGNTLVIVTADQAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEE
                     DSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK"
     gene            1076..1396
                     /gene="psiF"
                     /locus_tag="OHN27_000437"
     CDS             1076..1396
                     /gene="psiF"
                     /locus_tag="OHN27_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCQ2284944.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            1498..2613
                     /gene="adrA"
                     /locus_tag="OHN27_000438"
                     /gene_synonym="dgcC"
     CDS             1498..2613
                     /gene="adrA"
                     /locus_tag="OHN27_000438"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCQ2284945.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2630..3439)
                     /gene="proC"
                     /locus_tag="OHN27_000439"
     CDS             complement(2630..3439)
                     /gene="proC"
                     /locus_tag="OHN27_000439"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCQ2284946.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            3559..4023
                     /locus_tag="OHN27_000440"
     CDS             3559..4023
                     /locus_tag="OHN27_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCQ2284947.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAAINPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRGQM"
     gene            4200..4724
                     /gene="aroL"
                     /locus_tag="OHN27_000441"
     CDS             4200..4724
                     /gene="aroL"
                     /locus_tag="OHN27_000441"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCQ2284948.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            4774..4965
                     /gene="yaiA"
                     /locus_tag="OHN27_000442"
     CDS             4774..4965
                     /gene="yaiA"
                     /locus_tag="OHN27_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCQ2284949.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            5223..5900
                     /locus_tag="OHN27_000443"
     CDS             5223..5900
                     /locus_tag="OHN27_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCQ2284950.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNRGYDVIILMSTANI
                     SSMTARNTILLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            5972..6256
                     /gene="ppnP"
                     /locus_tag="OHN27_000444"
     CDS             5972..6256
                     /gene="ppnP"
                     /locus_tag="OHN27_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCQ2284951.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            6411..6746
                     /locus_tag="OHN27_000445"
                     /pseudo
     CDS             6411..6746
                     /locus_tag="OHN27_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            6743..6826
                     /locus_tag="OHN27_000446"
     CDS             6743..6826
                     /locus_tag="OHN27_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2284952.1"
                     /translation="MTQRPWSKLQRKKHNIAALKIIARRSE"
     gene            complement(6904..7815)
                     /gene="rdgC"
                     /locus_tag="OHN27_000447"
     CDS             complement(6904..7815)
                     /gene="rdgC"
                     /locus_tag="OHN27_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCQ2284953.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            7940..8848
                     /gene="mak"
                     /locus_tag="OHN27_000448"
     CDS             7940..8848
                     /gene="mak"
                     /locus_tag="OHN27_000448"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCQ2284954.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(9093..10277)
                     /gene="araJ"
                     /locus_tag="OHN27_000449"
     CDS             complement(9093..10277)
                     /gene="araJ"
                     /locus_tag="OHN27_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCQ2284955.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIHDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(10403..13546)
                     /gene="sbcC"
                     /locus_tag="OHN27_000450"
     CDS             complement(10403..13546)
                     /gene="sbcC"
                     /locus_tag="OHN27_000450"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCQ2284956.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALVDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPGGLALTVTGEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            complement(13543..14745)
                     /gene="sbcD"
                     /locus_tag="OHN27_000451"
     CDS             complement(13543..14745)
                     /gene="sbcD"
                     /locus_tag="OHN27_000451"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCQ2284957.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            14935..15624
                     /gene="phoB"
                     /locus_tag="OHN27_000452"
     CDS             14935..15624
                     /gene="phoB"
                     /locus_tag="OHN27_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCQ2284958.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            15682..16977
                     /gene="phoR"
                     /locus_tag="OHN27_000453"
     CDS             15682..16977
                     /gene="phoR"
                     /locus_tag="OHN27_000453"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCQ2284959.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMNEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            17384..18703
                     /gene="brnQ"
                     /locus_tag="OHN27_000454"
     CDS             17384..18703
                     /gene="brnQ"
                     /locus_tag="OHN27_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCQ2284960.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            18779..20152
                     /gene="proY"
                     /locus_tag="OHN27_000455"
     CDS             18779..20152
                     /gene="proY"
                     /locus_tag="OHN27_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCQ2284961.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            20308..22125
                     /gene="malZ"
                     /locus_tag="OHN27_000456"
     CDS             20308..22125
                     /gene="malZ"
                     /locus_tag="OHN27_000456"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCQ2284962.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKTFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(22130..22711)
                     /gene="acpH"
                     /locus_tag="OHN27_000457"
     CDS             complement(22130..22711)
                     /gene="acpH"
                     /locus_tag="OHN27_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCQ2284963.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASRKAL"
     gene            22804..23874
                     /gene="queA"
                     /locus_tag="OHN27_000458"
     CDS             22804..23874
                     /gene="queA"
                     /locus_tag="OHN27_000458"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCQ2284964.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            23930..25057
                     /gene="tgt"
                     /locus_tag="OHN27_000459"
     CDS             23930..25057
                     /gene="tgt"
                     /locus_tag="OHN27_000459"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCQ2284965.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            25080..25412
                     /gene="yajC"
                     /locus_tag="OHN27_000460"
     CDS             25080..25412
                     /gene="yajC"
                     /locus_tag="OHN27_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCQ2284966.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            25440..27287
                     /gene="secD"
                     /locus_tag="OHN27_000461"
     CDS             25440..27287
                     /gene="secD"
                     /locus_tag="OHN27_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCQ2284967.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            27298..28269
                     /gene="secF"
                     /locus_tag="OHN27_000462"
     CDS             27298..28269
                     /gene="secF"
                     /locus_tag="OHN27_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCQ2284968.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVMKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            28398..28745
                     /locus_tag="OHN27_000463"
     CDS             28398..28745
                     /locus_tag="OHN27_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCQ2284969.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(28922..29806)
                     /gene="tsx"
                     /locus_tag="OHN27_000464"
     CDS             complement(28922..29806)
                     /gene="tsx"
                     /locus_tag="OHN27_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCQ2284970.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(30105..30644)
                     /locus_tag="OHN27_000465"
     CDS             complement(30105..30644)
                     /locus_tag="OHN27_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCQ2284971.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            30795..31244
                     /gene="nrdR"
                     /locus_tag="OHN27_000466"
     CDS             30795..31244
                     /gene="nrdR"
                     /locus_tag="OHN27_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCQ2284972.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            31248..32351
                     /gene="ribD"
                     /locus_tag="OHN27_000467"
     CDS             31248..32351
                     /gene="ribD"
                     /locus_tag="OHN27_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCQ2284973.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            32440..32910
                     /gene="ribE"
                     /locus_tag="OHN27_000468"
     CDS             32440..32910
                     /gene="ribE"
                     /locus_tag="OHN27_000468"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCQ2284974.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            32930..33349
                     /gene="nusB"
                     /locus_tag="OHN27_000469"
     CDS             32930..33349
                     /gene="nusB"
                     /locus_tag="OHN27_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCQ2284975.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            33427..34404
                     /gene="thiL"
                     /locus_tag="OHN27_000470"
     CDS             33427..34404
                     /gene="thiL"
                     /locus_tag="OHN27_000470"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCQ2284976.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            34382..34900
                     /gene="pgpA"
                     /locus_tag="OHN27_000471"
     CDS             34382..34900
                     /gene="pgpA"
                     /locus_tag="OHN27_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCQ2284977.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(34954..35928)
                     /gene="yajO"
                     /locus_tag="OHN27_000472"
     CDS             complement(34954..35928)
                     /gene="yajO"
                     /locus_tag="OHN27_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCQ2284978.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(36108..37970)
                     /gene="dxs"
                     /locus_tag="OHN27_000473"
     CDS             complement(36108..37970)
                     /gene="dxs"
                     /locus_tag="OHN27_000473"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCQ2284979.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(37995..38894)
                     /gene="ispA"
                     /locus_tag="OHN27_000474"
     CDS             complement(37995..38894)
                     /gene="ispA"
                     /locus_tag="OHN27_000474"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCQ2284980.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(38894..39136)
                     /gene="xseB"
                     /locus_tag="OHN27_000475"
     CDS             complement(38894..39136)
                     /gene="xseB"
                     /locus_tag="OHN27_000475"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCQ2284981.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            39342..40790
                     /gene="thiI"
                     /locus_tag="OHN27_000476"
     CDS             39342..40790
                     /gene="thiI"
                     /locus_tag="OHN27_000476"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCQ2284982.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(40844..41434)
                     /gene="yajL"
                     /locus_tag="OHN27_000477"
     CDS             complement(40844..41434)
                     /gene="yajL"
                     /locus_tag="OHN27_000477"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCQ2284983.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWLDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(41397..42308)
                     /gene="panE"
                     /locus_tag="OHN27_000478"
     CDS             complement(41397..42308)
                     /gene="panE"
                     /locus_tag="OHN27_000478"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCQ2284984.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            42476..42967
                     /gene="yajQ"
                     /locus_tag="OHN27_000479"
     CDS             42476..42967
                     /gene="yajQ"
                     /locus_tag="OHN27_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCQ2284985.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(43095..44459)
                     /locus_tag="OHN27_000480"
     CDS             complement(43095..44459)
                     /locus_tag="OHN27_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2284986.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLTVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLTVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(44608..45498)
                     /gene="cyoE"
                     /locus_tag="OHN27_000481"
     CDS             complement(44608..45498)
                     /gene="cyoE"
                     /locus_tag="OHN27_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCQ2284987.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(45510..45839)
                     /locus_tag="OHN27_000482"
     CDS             complement(45510..45839)
                     /locus_tag="OHN27_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCQ2284988.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(45839..46453)
                     /locus_tag="OHN27_000483"
     CDS             complement(45839..46453)
                     /locus_tag="OHN27_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCQ2284989.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(46443..48434)
                     /gene="cyoB"
                     /locus_tag="OHN27_000484"
     CDS             complement(46443..48434)
                     /gene="cyoB"
                     /locus_tag="OHN27_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCQ2284990.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLISYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(48456..49403)
                     /gene="cyoA"
                     /locus_tag="OHN27_000485"
     CDS             complement(48456..49403)
                     /gene="cyoA"
                     /locus_tag="OHN27_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCQ2284991.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGT
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(49864..51339)
                     /gene="ampG"
                     /locus_tag="OHN27_000486"
     CDS             complement(49864..51339)
                     /gene="ampG"
                     /locus_tag="OHN27_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCQ2284992.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(51383..51961)
                     /locus_tag="OHN27_000487"
     CDS             complement(51383..51961)
                     /locus_tag="OHN27_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2284993.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            52266..52583
                     /gene="bolA"
                     /locus_tag="OHN27_000488"
     CDS             52266..52583
                     /gene="bolA"
                     /locus_tag="OHN27_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCQ2284994.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            52927..54225
                     /gene="tig"
                     /locus_tag="OHN27_000489"
     CDS             52927..54225
                     /gene="tig"
                     /locus_tag="OHN27_000489"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCQ2284995.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            54471..55094
                     /gene="clpP"
                     /locus_tag="OHN27_000490"
     CDS             54471..55094
                     /gene="clpP"
                     /locus_tag="OHN27_000490"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCQ2284996.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            55220..56494
                     /gene="clpX"
                     /locus_tag="OHN27_000491"
     CDS             55220..56494
                     /gene="clpX"
                     /locus_tag="OHN27_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCQ2284997.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            56682..59036
                     /gene="lon"
                     /locus_tag="OHN27_000492"
     CDS             56682..59036
                     /gene="lon"
                     /locus_tag="OHN27_000492"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCQ2284998.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            59245..59517
                     /gene="hupB"
                     /locus_tag="OHN27_000493"
     CDS             59245..59517
                     /gene="hupB"
                     /locus_tag="OHN27_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCQ2284999.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            59709..61580
                     /gene="ppiD"
                     /locus_tag="OHN27_000494"
     CDS             59709..61580
                     /gene="ppiD"
                     /locus_tag="OHN27_000494"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCQ2285000.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            61731..62102
                     /locus_tag="OHN27_000495"
     CDS             61731..62102
                     /locus_tag="OHN27_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCQ2285001.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            62196..62594
                     /gene="fadM"
                     /locus_tag="OHN27_000496"
     CDS             62196..62594
                     /gene="fadM"
                     /locus_tag="OHN27_000496"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCQ2285002.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(62646..63341)
                     /gene="queC"
                     /locus_tag="OHN27_000497"
     CDS             complement(62646..63341)
                     /gene="queC"
                     /locus_tag="OHN27_000497"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCQ2285003.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(63406..65106)
                     /locus_tag="OHN27_000498"
     CDS             complement(63406..65106)
                     /locus_tag="OHN27_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCQ2285004.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            65206..66024
                     /gene="cof"
                     /locus_tag="OHN27_000499"
     CDS             65206..66024
                     /gene="cof"
                     /locus_tag="OHN27_000499"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCQ2285005.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            66177..66635
                     /gene="decR"
                     /locus_tag="OHN27_000500"
     CDS             66177..66635
                     /gene="decR"
                     /locus_tag="OHN27_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCQ2285006.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            66665..68437
                     /locus_tag="OHN27_000501"
     CDS             66665..68437
                     /locus_tag="OHN27_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCQ2285007.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            68430..70211
                     /locus_tag="OHN27_000502"
     CDS             68430..70211
                     /locus_tag="OHN27_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCQ2285008.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            70392..70730
                     /gene="glnK"
                     /locus_tag="OHN27_000503"
     CDS             70392..70730
                     /gene="glnK"
                     /locus_tag="OHN27_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCQ2285009.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            70760..72046
                     /gene="amtB"
                     /locus_tag="OHN27_000504"
     CDS             70760..72046
                     /gene="amtB"
                     /locus_tag="OHN27_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCQ2285010.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(72095..72955)
                     /gene="tesB"
                     /locus_tag="OHN27_000505"
     CDS             complement(72095..72955)
                     /gene="tesB"
                     /locus_tag="OHN27_000505"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCQ2285011.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            73173..73745
                     /locus_tag="OHN27_000506"
     CDS             73173..73745
                     /locus_tag="OHN27_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCQ2285012.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(73776..74087)
                     /locus_tag="OHN27_000507"
     CDS             complement(73776..74087)
                     /locus_tag="OHN27_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCQ2285013.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            74250..74346
                     /gene="ffs"
                     /locus_tag="OHN27_000508"
     ncRNA           74250..74346
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OHN27_000508"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            74466..74819
                     /locus_tag="OHN27_000509"
     CDS             74466..74819
                     /locus_tag="OHN27_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCQ2285014.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(74861..76411)
                     /gene="pdeB"
                     /locus_tag="OHN27_000510"
     CDS             complement(74861..76411)
                     /gene="pdeB"
                     /locus_tag="OHN27_000510"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCQ2285015.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVAYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLRDMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(76575..77045)
                     /locus_tag="OHN27_000511"
     CDS             complement(76575..77045)
                     /locus_tag="OHN27_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCQ2285016.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(77161..77712)
                     /gene="maa"
                     /locus_tag="OHN27_000512"
     CDS             complement(77161..77712)
                     /gene="maa"
                     /locus_tag="OHN27_000512"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCQ2285017.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(77883..78101)
                     /gene="hha"
                     /locus_tag="OHN27_000513"
     CDS             complement(77883..78101)
                     /gene="hha"
                     /locus_tag="OHN27_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCQ2285018.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(78127..78501)
                     /gene="tomB"
                     /locus_tag="OHN27_000514"
     CDS             complement(78127..78501)
                     /gene="tomB"
                     /locus_tag="OHN27_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCQ2285019.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        19072 a        19629 c        21120 g        19000 t
ORIGIN      
        1 attctggaaa tggcaaaagc cgcaggtctg gcgaccggta acgtttctac cgcagagttg
       61 caggatgcca cgcccgctgc gctggtggca catgtgacct cgcgcaaatg ctacggtccg
      121 agcgcgacca gtgaaaaatg tccgggtaac gcgctagaaa aaggcgggag aggatcgatt
      181 accgaacagc tgcttaacgc tcgtgccgat gttacgcttg gcggcggcgc aaaaaccttt
      241 gctgaaacgg caaccgccgg tgaatggcag ggaaaaacgc tgcgtgaaca ggcacaggcg
      301 cgtggttatc agttggtgag cgatgctgcc tcactgaatg cggtgacgga agcgaaccag
      361 caaaaacccc tgctaggact gtttgctgac ggcaatatgc cagtgcgctg gcaaggaccg
      421 aaagcaacgt accacggcaa tatcgacaag cccgcagtta cctgtacgcc taatccgcaa
      481 cgtaatgaca gcgtaccgac cctggcgcag atgactgata aagccattga attgttgagt
      541 aaaaatgaga aaggcttttt cctgcaagtt gaaggtgcat caatcgataa acaggatcac
      601 gctgcgaatc cttgtgggca aattggcgag acggtcgatc tcgatgaagc cgtacaacgg
      661 gcgctggaat tcgctaaaaa ggagggtaac acgctggtca tagtcaccgc tgatcaagcc
      721 cacgccagcc agattgttgc gccggatacc aaagctccgg gcctcaccca ggcgctaaat
      781 accaaagatg gcgcagtgat ggtgatgagt tacgggaact ctgaagagga ttcacaagaa
      841 catacaggca gtcagttgcg tattgcggcg tatggccccc atgccgccaa tgttgttgga
      901 ctgaccgacc agaccgatct cttctacacc atgaaagccg ccctggggct gaaataaaac
      961 cgcgcccggc agtgaatttt cgctgccggg tggttttttt gctgttagca accagactta
     1021 atggcagatc acgggcgcaa acgctcatgg ttaaaacatg aagagggatg gtgctatgaa
     1081 aataacatta ctggttacct tgctttttgg tctggttttt ttaaccactg tcggcgctgc
     1141 cgagagaact ttaaccccac aacaacagcg tatgacctcc tgtaatcagc aggcgacggc
     1201 gcaggcgttg aaaggggatg ctcgtaagac ctacatgagt gattgcctga agaacagcaa
     1261 gtctgcgcct ggcgaaaaaa gtttgacgcc acagcagcaa aagatgcgcg aatgtaataa
     1321 tcaagcaacg caacaatctc tgaaagggga tgatcgtaat aagtttatga gtgcctgcct
     1381 caagaaagcc gcctgatacc tgatagtgct aacgggtgag ctacgaaaat ggctcacccg
     1441 aaatttcata cttctgcctt tagctccgtc tctataattt gggaaaattg tttctgaatg
     1501 ttcccaaaaa taatgaatga tgaaaacttt ttcaaaaaag cggcggcgca tggggaggaa
     1561 cctcctttaa ctcctcaaaa cgaacatcag cggtccgggc tgcgcttcgc ccgtcgcgtc
     1621 agactacccc gcgcggttgg cctggctggc atgttcttac cgattgcttc aacgctggtt
     1681 tcacacccgc cgccgggctg gtggtggctg gtgttggtcg gctgggcgtt cgtctggccg
     1741 catttagcct ggcagatagc gagcagggcc gtcgatccgc ttagccggga aatttacaat
     1801 ttaaaaaccg atgcagtatt agcgggaatg tgggtaggcg taatgggcgt aaacgtgctg
     1861 ccttccaccg cgatgttgat gattatgtgt ctgaatttga tgggggcagg cggcccccgt
     1921 ctgtttgtcg cgggtctggt gttgatggtg gtttcctgcc ttgtcaccct cgagctgacg
     1981 ggcattaccg tgtcgttcaa tagtgcgccg ctggaatggt ggctctccct tcccattatt
     2041 gtcatttatc ctctgctgtt tggctgggtc agctaccaga cggcaaccaa actggcggaa
     2101 cataaacgca ggttgcaggt catgagtacc cgcgacggca tgacgggcgt gtataaccga
     2161 cgtcattggg aaactatgtt acgcaatgaa tttgataact gtcggcgaca taatcgcgat
     2221 gcaacgttac tgattatcga tatcgaccat ttcaagagca tcaacgatac ctggggccat
     2281 gatgtgggcg atgaagcgat agtggcgctt acccgacagt tacaaattac cctgcgcggt
     2341 agcgatgtga ttggtcggtt tggcggcgat gagtttgcag taatcatgtc cggtacgcca
     2401 gctgagagcg ctattaccgc catgttacgg gtgcatgaag ggctaaatac attacgtttg
     2461 ccgaatacgc cacaggtaac tttacggatt agtgtggggg ttgcgccgct gaacccacaa
     2521 atgagtcact atcgtgagtg gttgaaatcg gcagatttgg cgctttacaa agcaaagaaa
     2581 gccggacgta accgcaccga agtggcggcc tgacgtcagg cgaaagtcat caggatttgc
     2641 tgagtttttc tgatttttcc atacacttcg tcatcgcttc gatcactgcg gcacggaagc
     2701 ctttctcttc cagtacgcgt accgcttcaa tggtggtgcc tcccggtgag cagaccatat
     2761 ctttcagtgc ccccggatgt tctcccgttt ccagcaccat ttttgcggaa cccattactg
     2821 cctgagcggc aaatttatac gcctgggcgc gtggcatccc gcccagcacg gcggcgtcgg
     2881 ccatcgcttc gataaacata aatacgtagg ctggcgaaga accgctcaca ccgaccaccg
     2941 ggtggatcat cggctcagca atcacttccg cttcgccaaa gcagcggaaa atattcagca
     3001 catcagcggt atcttctggg gttaccagcg cgtttggcgt tacggaggtc atcccggcat
     3061 taaccagcgc gggagtgttc ggcatggcgc ggataatttt ccggtcatgg cccagcgcgc
     3121 gggcaagttg gtcgagcgtg acacctgcag caatagaaac gaccagagag tctttattca
     3181 ggctggaggt gatttcgcta agcactttaa tcatgatgcc aggtttaacg gcagcaaaaa
     3241 tgatgtcggc gatttgcgcc acttcttgcg ccgattctgc ggcgttgatg ccgaactggt
     3301 catgcagggc ggcgacttta tccggggagg gggtatatac ccagatttgc cctggaagca
     3361 cctgaccgct ggcaatcaga ccgccgagaa tggcttttcc catattgccg cagccaataa
     3421 aaccgatttt cttttccatt gcctcactcc tgccgtgaaa ttcattgttt tgataatcgc
     3481 tggcagaagc ataaacagaa ctatgccgga aggcaaaagc gcgacacaat agaggattac
     3541 ccaacaaagg atgactttat gacaatttgg gtggatgccg acgcgtgtcc caatgtaatt
     3601 aaagagattt tgtatcgcgc ggcggaacgt atgcagatgc cgctggtact ggtagcaaac
     3661 cagagtttac gcgtgccgcc atcgcgattt attcgtacgc tgcgcgtcgc ggcaggtttc
     3721 gacgttgccg ataacgaaat tgtccggcag tgtgaagcgg gcgatttggt gatcaccgca
     3781 gatatacctt tggctgctga agccatcgag aaaggcgctg cggcgattaa tccgcgcggc
     3841 gaacgttaca cgccagcgac cattcgtgag cgcctgacga tgcgcgattt tatggatacc
     3901 ttacgtgcca gtgggatcca gaccggcgga ccagatagtc tttcacaacg tgaccgccag
     3961 gcctttgctg cggagctgga gaaatggtgg ctggaagtgc aacgtagtcg cggccaaatg
     4021 taatttatta tttacacttc attcttgaat atttattggt atagtaaggg gtgtattgag
     4081 attttcactt taagtggaat ttttccttta caatcgaaat tgtactagtt tgatggtatg
     4141 atcgctattc tcatgacacc ggctttcgcc gcattgcgac ctattgggga aaacccacga
     4201 tgacacaacc tctttttctg atcgggcctc ggggctgtgg taaaacaacg gtcggaatgg
     4261 cccttgccga ttcgcttaac cgtcggtttg tcgataccga tcagtggttg caatcacagc
     4321 tcaatatgac ggtcgcggag atcgtcgaaa gggaagagtg ggcgggattt cgcgccagag
     4381 aaacggcggc gctggaagcg gtaactgcgc catccaccgt tatcgctaca ggcggcggca
     4441 ttattctgac ggaatttaat cgtcacttca tgcaaaataa cgggatcgtg gtttatttgt
     4501 gtgcgccagt atcagtcctg gttaaccgac tgcaagctgc accggaagaa gatttacggc
     4561 caaccttaac gggaaaaccg ctgagcgaag aagttcagga agtgctggaa gaacgcgatg
     4621 cgctatatcg cgaagttgcg catattatca tcgacgcaac aaacgaaccc agccaggtga
     4681 tttctgaaat tcgtagcgcc ctggcacaga cgatcaattg ttgattttcg agcgcctata
     4741 cttaacgttc atcccgtgaa ataaggaaga acgatgccaa cgaaaccgcc ttatcctcgt
     4801 gaagcatata tagtgacgat tgaaaaagga aaaccaggac agacggtaac ctggtaccaa
     4861 ctcagagccg atcatcctaa accagactcg ttgatcagtg aacatccgac ggctcaggaa
     4921 gcgatggatg cgaaaaaacg ctatgaggac cctgacaaag agtgaccgca tcagactgct
     4981 cggaagggat tctgagtgcc actacaaggg atctgcgtca catttttcat aattcatgtt
     5041 tttctaataa ttagaatatt aaacaataac aatccattac tggaatcatt tggaatcttt
     5101 acattatgcc gtgcacgtct gctgctacgc tttttgtcat ttgtagcaca agtaagtgtc
     5161 agcagtggtg cttcacactt gcccggtaat taacgacgaa agaaaagtaa ggtggatgaa
     5221 caatgagtgc gtcgttggcg atcctgacca tcggcattgt acctatgcag gaagttttgc
     5281 cgctcctgac ggaatacatt gacgaagata atatttccca tcatagcctg ctggggaagt
     5341 taagtcgtga agaagtgatg gcggagtacg cgccagaagc aggcgaagac accattctca
     5401 cattattaaa tgacaaccag ctggcccatg tttcgcgtcg caaagtggag cgtgacctgc
     5461 aaggtgtggt tgaagtactc gataatcggg gttacgacgt cattatatta atgagtacgg
     5521 caaacattag tagtatgact gcgcgtaata cgatccttct tgagccgtcg cggatattgc
     5581 caccgctggt ttcctctatt gttgaagatc atcaagtggg ggttatcgtt ccggttgagg
     5641 agatgctgcc cgttcaggcg caaaaatggc aaattttgca gaaatcgccg gtattttcat
     5701 tgggtaaccc cattcatgat tcagaacaaa aaatcattga tgccgggaaa gaattacttg
     5761 caaaaggggc tgatgtcatc atgctggatt gtttaggatt tcaccaacgt catcgcgatt
     5821 tactgcaaaa acagctcgat gttcctgtct tgctgtctaa cgtattgatt gcacggctgg
     5881 ctgcggaatt actggtgtaa ttttgcgtga cagtcagcgc ctctggcccc tatagtgaag
     5941 tagatattca actaccagac agggccagtt tatgcttcaa agtaatgagt acttttccgg
     6001 caaagtgaaa tcgatcggct tttccagcag cagcactggt cgcgccagtg tgggtgttat
     6061 ggttgaaggc gaatacacct tcagcaccgc tgagccggaa gagatgacgg taatcagtgg
     6121 cgcgctgaat gtgttactgc ctgacgcgac cgactggcaa gtgtatgaag ccggttcggt
     6181 gtttaatgtt cccggtcaca gcgagtttca tctgcaagtt gccgaaccca cctcttatct
     6241 gtgtcgctat ctgtaattcc tcgccttccc cttgaacggg agggcatttt tctgaaatat
     6301 cctctcataa acccataata atatttcctt tgctgcgatt ttttcgattt ccggtatatt
     6361 cataattcat caaggttgat ataaatatca gtgaagatct ccatgtattt tttgcggtac
     6421 tggctacgat aaaagataaa tcagatgatg aatggtggcg tgcattgctg caaagtcgca
     6481 atgatcattt gcgccaaacg gcattgcgca acgcacatac cccggcgttg ttgttaacga
     6541 cattgactga gcctcaggat cggtcgctgg ctattaataa tccacagctg tctgccgatg
     6601 tgaaaacggc gtggttaaaa gaggatccat cattactctt atttgtcgaa caacccgatc
     6661 tttcgcagtt acgtgattta gtgaaaaccg gggcaacgcg gaaaattcgc agtgaagcgc
     6721 gtcaccggct tgaggaaaaa caatgacaca acgtccatgg agcaagttac agcgtaaaaa
     6781 gcataatatt gctgcgctaa aaattattgc tcgccgtagc gaataattat atgcctggtg
     6841 tggcttcgta cgccggataa gacgcggcag gcgtcgcatc cggcattaaa ggaaaatcag
     6901 caattaacgt tgtgcttcgc cacctaatcc ttcaatcagg ttttgaatta acgctgccag
     6961 ttcaccagtc atcaggatga aatcggcatc aaaacgctgg gcgaaatctt cacggtcgat
     7021 atcttcgttt tgatcgcgca gctcgtcgca gaacttcaga cgcttgagcg aaccatcgtc
     7081 gcacatcaca aactgaatgc gctgctgcca gtcgagcgcc agtttagtca ccacttttcc
     7141 ggcttcaatg tgattggtga tctcttcgct ggtcagatct tgtttcttcg cgcggatcac
     7201 gccgccatct tccagcaacg atttcagctc ggcttcatca agcagctgga agccctgtgc
     7261 cgcactaccg gagcgaaccc attcggtcag cgtcagttca atcgggtttt ccatgctcaa
     7321 tggtacaacc ggtaacgacc ccaggctttt acgcagcagt gccagcgtat cttccgcttt
     7381 tttggcactg gcgcagtcca ccataatcaa accgttaacc gtgtcgatcc acatcattgt
     7441 ctggctaaaa cggctgaaag cacgcggcaa cagagagtgc agcacttcgt ctttcagcga
     7501 atctttttcg gttttcttca gcttacgcgc ctgttccgct tccagtttgg cgattttcgc
     7561 ttccagcgcc tgtttaatca ccggagacgg gaggattttt tcttctttgc gcgcgcagat
     7621 aacaatttga ccattggcaa cgtgcgttaa cgcatcgctg tgcgatccca tcggaggaac
     7681 ccagcccatc ttcgccatgt cctggctgcc gcatggggta aatgccatcg aggctagctg
     7741 tttttccatc tcttctgcac gcagcgaaat ctcgcggcta agacggtaaa ccattaaatt
     7801 tttgaaccac agcatgataa tttccacggc cttgtcgtta aatttagcgg gcatgataac
     7861 gaattgtcgg cggccttgca ttgccaatcc ggttgtccgt ctctacgcta ttgatattga
     7921 aaaaaataag gagagtaccg tgcgtatagg tatcgattta ggcggcacca aaactgaagt
     7981 gattgcactg ggcgatgcag gggagcagtt gtaccgtcat cgtctgccca cgccgcgtga
     8041 tgattaccgg cagactattg aaacgatcgc cacgttggtt gatatggcgg agcaggcgac
     8101 agggcagcgc ggaacggtag gtatgggcat tccaggttcg atttcgcctt acaccggcgt
     8161 ggtgaaaaat gccaattcaa cctggctcaa cggtcagcca ttcgataaag acttaagcgc
     8221 gaggttgcag cgggaagtgc ggctggcaaa tgatgccaac tgtctggcgg tttcagaagc
     8281 agtagatggc gcggcagcgg gagcgcagac ggtatttgcc gtgattatcg gcacgggatg
     8341 cggcgcgggc gtggcattca atgggcgggc gcatatcggc ggcaatggca cggcaggtga
     8401 gtggggacac aatccgctac cgtggatgga cgaagacgaa ctgcgttatc gcgaggaagt
     8461 cccttgttat tgcggtaaac aaggttgtat tgaaaccttt atttcgggca cgggattcgc
     8521 gacggattat cgtcgtttga gcggacatgc gctgaaaggc agtgaaatta tcagcctggt
     8581 tgaagaaagc gatccggtag cggaactggc attgcgtcgc tacgagctgc ggctggcaaa
     8641 atcgctggca catgtcgtga atattctcga tccggatgtg attgtgctgg ggggcgggat
     8701 gagcaatgta gaccgtttat atcaaacggt tccgcagttg attaaacaat ttgtcttcgg
     8761 cggcgaatgt gaaacgccgg tgcgtaaggc gaagcacggt gattccagcg gcgtacgcgg
     8821 cgctgcgtgg ttatggccac aagagtaaaa aacgtaggca attggcgcat catgcctgat
     8881 gcgacgcttg ccgcgtctta tcaggcctac aaaaggtgcc agaaccgtaa gccggataag
     8941 gcgttcacgc cgcatccggc aataagtgct ccgatgcctg atgcgacgct tgtcgcgtct
     9001 tatcaggcct acaaaatgtg ccagaaccgc gtagggcgga taaggcgttc acgccgcatc
     9061 cggcaataag caacgcgcac cgtgacagta atctacccca gtggtttcgc cagcaccgga
     9121 gtatccgccg cttgctggcg cttatagcga ccatacagca gcaatgacga catcgcagca
     9181 aacgaaagca gggcggcagg cagcgccacg taattatatg ccagccccag cgtcagcatc
     9241 ataccaccgc aatatgcgcc gacggcgcta ccgaggttaa acgctatttg cccacctgcg
     9301 gcacctaata actctccgcc tttggcgttt tgtagtaaca atatttgcag aggtgctgaa
     9361 agggcaaata atcccgcgca acaaataaaa gcaaaaataa gcgacgttgt tttcatgccg
     9421 ccgcagaaaa agagcatcag cagtgccagt acaatgataa agtcagtcac tgctgcaatg
     9481 cgcagtggtg aataacgtcc tgaaatcctg ccacttagca tatttcccag caccatccct
     9541 agcccaacta acatcataat aaaggtcatc gccgtttccg aaaaaccgga aataaacatc
     9601 atgtatggct ttacgtagct gaaccaggca aacacacctg cgttgccaaa catggtggcg
     9661 gcgaaaatta accacggggc cgggctgcgc aaaaagtgaa attgttcgcg cagctttcct
     9721 ttcgcctcgt cgtgaatatc tggcacccaa aaatagaccg atgccatcac cgcaatatta
     9781 aaaacagcga tcaataaaaa ggtgtagcgc cagctaaatt cctgacttaa atacgttccc
     9841 agcggaatgc ccagcaaatt ggcgactgtc atcccggaaa ccatccccgc cacggcggcg
     9901 gtgacttttc cgggtttgat aatttttgat aacacgatcg ctccgacgcc aaaaaatgcg
     9961 ccatgcggaa agccggaaac caggcgacca atggcaagca tcaggtaaga cgaagagagc
    10021 gtgaacatgg cgttgccaat gacgcacaac gccaccagaa acaacaagat atgtttgagt
    10081 gagtagcggc tggaaaagag tgcgatgatt ggcgcaccga ccaccacccc cagcgcataa
    10141 tacgagatca tatgcccggc agcaggaatc gaaattccta cgttatgcgc cagctccgtg
    10201 agcacgccca taatgccaaa ttcggccatc cccaaaccaa acgtgcccag agccaaagat
    10261 aaaatgactt ttttcatacc accatcacaa ccaccagaac ggctactaat caccgtagcc
    10321 aataaagtga tagcttgcag gataacgacc agcgaggcca agagataatt cgcccctctg
    10381 tattcattat cctgctgaat agttatttca ctgcaaacgc actttccagt ttgctgtagc
    10441 ccaggccgtt gatctttttc actttgatct gcaccggaat acgctctttc atcgcttcaa
    10501 cgtggctaat cacaccgatg gttttgccac tggcattcag ggcatccagc gcatcaaggg
    10561 cggtatccag cgtttcgcta tccagtgtgc caaaaccttc atcgaggaac agcgaatcaa
    10621 tacgcgtttt atggctgacc agatctgaaa gcgccagcgc cagcgccaga ctgacgagga
    10681 aactttcgcc gccagaaagg gtacgggtgt cacgtaccgc atctgcctgc caggtatcaa
    10741 caacctcgac ttccagcgcc tcgctggctt tgcgctgtaa cagatagcgc ccgtgcagcc
    10801 gggtaagttg ctgattagcg agatggacta aattatccag cgtcagcccc tgggcaaact
    10861 tgcggaattt atcgccctct ttggaaccta ttagcgaatt cagatatccc cagtcctcaa
    10921 cctgctgcgt catttgagca atttgctgca ttaaggtttg ttgttgctga cggttatctg
    10981 catcctgctt cagctgctgg cgaatctcgc cttgactcgt ggtgttttca cgcaactttt
    11041 ggtgagtttg cgctaactct tgctgaatct gctcccccgt cacagtgaga gccaacccgc
    11101 caggtcggtg ttgctgatgc tgtgccagcg tttctgctgt ctgagtgacc agcgtttgcg
    11161 cctgacggcg ctggttttcc agattctgct tgagctgttc cagctgcgtt agtgtttgtt
    11221 catccactag cgccgcaagg aacgcctgct gatcgtcaaa gacgctggcc tgtagcgcgg
    11281 tgtcaaactg cgcctgggct ttttgcagac tttgcgccgc cagaacgtcc tgttgctgta
    11341 acgtctgctg ctggctgtgt aatgcgagac attgttcatg aacctgccgc cagttatcca
    11401 gcgccacagt ttcttcgctg tgcgggagat catcactttg cggcaacgtt tccagaatcg
    11461 gcgtcagctg ctgaatacgg ttttgcagcg cggttaattc gttctggcgt tgctgccagc
    11521 tctgcgcttc ttgctgacgt gtcgccaacc agctctcttc ttcatcttcc tgtggcaatg
    11581 tcagtgcata acccgccaat gccgttaaga gctgctgctg gcgttgttca atttgctgtt
    11641 gatactggat aatttgctga ttatgcgcgg caatctgccc ttgtaattca tgtcgttggc
    11701 tgagtaaccg cagctggcgt tcgtgctcat cttgtgcatc cagccacggt tgaatatcgt
    11761 cctgtggctg caaggtgata ttgaggctgg ccgtgacggc ttgccattgt tgagtaagtg
    11821 cttgctcatc ttgtcggagg ctttgcgctt cgttttcatc gcgctgaagc tgcttcgtta
    11881 atgcatccag ttgcccgcgc agcgccgcac cttcttcgcc gagcttttta acttcgtttt
    11941 ccagcgccag taatcgagac tgattaacac caggctccag cgcctgatac gcctcgaccg
    12001 ccgggtggct ggtggaacca caaagtggac aaggctgacc cgcctgtaac tgtgcacgct
    12061 gggcttccag cgttttgatg cgcgcttcct gctcgcaaat ggttttcaca tcggcaagtt
    12121 gctgcgtctt ttctttataa cgctggcgca tttcgttaag tgcgacatta cgttgcgtct
    12181 gttccagagt gacattctgg atagtgacca ttaactgcgc cagacgtttt tgttggggaa
    12241 caatctgccc atgcagcgcg accaggcgct gacgcagtgg gcgttgctca gcatgttgcg
    12301 ccagggcggt agcaacttca tcggcggtta acgtcaacgt gatcgccgca agcgcattaa
    12361 gtttttgctc agcatgggtt aactgttgct gccattgccg cagatgctcg cgatcgctgg
    12421 tttgttggga gaactgcgca cgccaacccg ccagttcgtt gttccactga cggaagcggt
    12481 cgtgttcctg taaccaggta ttcaggcttt gttgctgctg ctgtaattct gctgactgct
    12541 tcgccgcgtg gtggcgaatg ctcgcgcgaa gcgccattgt gctctgtaag cgagtattta
    12601 cttcttcaat ctgctggcga gtatgcgcca gcgccgcgct gtgttctgcg atgcgttccc
    12661 agtgcggacg aagatttcgt gccggttgtg ccagactaag cgccgccagt tgaggttgcg
    12721 ctttttcttc ttcggctaac gcctgttgca aggcctgctg acggcggctg gcttcttgct
    12781 gcaattcgtc ctgacgcgtt aaccagttta gcgattgttg ttcttgctgc tgcgcggtaa
    12841 ttaactgttt ttcttcgtca gtaagtacct gcaaactcgc tgtcagcgat tgcacttgtt
    12901 ccggcgtgag caacgtgacg ccgctggcct gcgcttgcag cttctccagc tctgtgcggg
    12961 ccgatttgtg ctgctcaaaa accatcgccg agatttgccc gtagatttca gtgccggtta
    13021 actcctcgag caattccgcg cgttctttgg gtttggcatt caggaaggca gcaaattgcc
    13081 cctgcgaaag cagcatcgaa cgggtgaagc gcccgtaatc cagcccggtt aacgtcgctg
    13141 tcagttccag cttatctttc actttgtcgg cgagaatttt gccgtcggcg cagcgcgcca
    13201 gctctacgcg tggcacctgc aaattaccgt cgggttggtt acgcgcccga ttctggctcc
    13261 agaatgcacg gtacgcttca cctttcactt caaactccac ctccgccaga cattcggcgg
    13321 tatcgcgggt catgagatca ttttgcgatt gtgaaacgtt agagagacgc ggagtttcgt
    13381 gatacagcgc cagacaaatg gcgtccagca gggtggtttt ccccgcacct gttgggccgg
    13441 taatagcaaa cagcccgttg ctggcgaacg gctcgcgggt gaaatcaatc ttccattcgc
    13501 cttttaatga gttcaggttt ttcaggcgca ggctgagaat tttcatgctt cgtgttctcc
    13561 ggcgagggta tgcaacgtcg tggtgaaaag atgctgcaga cgttgctgct gcgattcatc
    13621 cagttcttcc agtgccagac ggcgattgaa cacctcttcg acgctgagtt cgctgagggt
    13681 ttcacgctgt tggctggcta acacacgctc gcgctgttca cgactccgac gtaccagcaa
    13741 tacttcgaca ggcaatgatt cggttaatgc ctggattttg cgctgaatat catgcagata
    13801 ctcatcagta gtgatttcga tatccagcca gacaggtggc tcctgcgata catcgcgcca
    13861 ctgttccagc tgtgcggtaa tcgacgccag atcgcctttc agcactgcca tgggttgcgt
    13921 taccggtacg ttcaggtttt ccacgctctc taatttgccg tttgaaaatg tcaccagatg
    13981 gacatattta ctcttaccgc attcatcaaa actcagtgga atgggggagc cgcaatagcg
    14041 aacatgttcc atgccgccaa taatctgtgc gcggtgaata tgcccgagcg cgatgtagtc
    14101 ggctggtgga aagttttgtg ccggaaacgc gtccagcgtg ccaatataaa tgtcacgcac
    14161 ggcgtcactt ttactggccc ccacggtcgt taaatgtccc gtggcgatga tgggcagagg
    14221 ctgatcgccg cgcagtttgc aggcatcggc atagtgttgt tggtaataat cggtaatcgc
    14281 tgccagtaaa tgctgctgtt tttcaatacc gttaagcccc gcctggctgg taataatgtc
    14341 acgcggacgt aaaaacggaa tggggcacag cactgcgcct ggcgtcccgt cgcgacgagg
    14401 caagatttgc ggcgcatgtc cggcgctggc gaccacggta gtattgagga acgccatgat
    14461 atcgcgcgat tcattcagcg tggcgaccga gtcatggttt cctgccagta ccaccagatg
    14521 acagccagtt tgctgtaaat tgacaacaaa acggttgtat aacgtgcggg cgtaactggg
    14581 cggcgagccg gtatcgaaaa catcaccggc aacaataatc gcatccacct gatgggtttg
    14641 tgctgtctcc agcagccagt caagaaaagc ctgatgttca gcttcgcggc ttttactgta
    14701 gaagttctgg ccgagatgcc agtctgaggt gtgaaggatg cgcataacgg ttccctggcg
    14761 aaaaagcatg ggcgcgatta tacccaaaca gatgtgccat ttgctttttt ctgcgccacg
    14821 gaaatcaata acctgaagat atgtgcgacg agcttttcat aaatctgtca taaatctgac
    14881 gcataatgac gtcgcattaa tgatcgcaac ctatttatta caacagggca aatcatggcg
    14941 agacgtattc tggtcgtaga agatgaagct ccaattcgcg aaatggtctg cttcgtgctc
    15001 gaacaaaatg gctttcagcc ggtcgaagcg gaagattatg acagtgctgt gaatcaactg
    15061 aatgaaccct ggccggattt aattctcctt gactggatgt tacctggcgg ctccggtatc
    15121 cagttcatca aacacctcaa gcgcgagtcg atgacccggg atattccagt ggtgatgttg
    15181 accgccagag gggaagaaga agatcgcgtg cgcggccttg aaaccggcgc ggatgactat
    15241 atcaccaagc cgttttcgcc gaaggagctg gtggcgcgaa tcaaagcggt aatgcgccgt
    15301 atttcgccaa tggcggtgga agaggtgatt gagatgcagg gattaagtct cgacccgaca
    15361 tctcaccgag tgatggcggg cgaagagccg ctggagatgg ggccgacaga atttaaactg
    15421 ctgcactttt ttatgacgca tcctgagcgc gtgtacagcc gcgagcagct gttaaaccac
    15481 gtctggggaa ctaacgtgta tgtggaagac cgcacggtcg atgtccacat tcgtcgcctg
    15541 cgtaaagcac tggagcccgg cgggcatgac cgcatggtgc agaccgtgcg cggtacagga
    15601 tatcgttttt caacccgctt ttaacgcctt gctcatcgga cgcagagcag ggcttatgat
    15661 ttcttaactg gagtatctta cgtgctggaa cggctgtcgt ggaaaaggct ggtgctggag
    15721 ctgctacttt gctgcctccc ggctttcatc ctgggtgcat tttttggtta cctgccctgg
    15781 tttttgctgg catcggtaac aggactgctt atctggcatt tctggaattt attgcgcctt
    15841 tcatggtggc tgtgggtgga tcgcagtatg accccgccac cggggcgtgg tagctgggaa
    15901 ccgctactat acggcttaca ccagatgcag ctgcgaaata aaaaacgccg ccgtgaactg
    15961 ggcaatctga ttaaacgctt tcgtagcggc gcggagtcgc tgcccgacgc ggtggtgctg
    16021 accacggaag agggcggtat tttctggtgt aacggtctgg cgcaacaaat tcttggtttg
    16081 cgctggccgg aagataacgg gcagaacatc cttaacctac tgcgttaccc ggagtttacg
    16141 caatatctga aaacgcgtga tttttctcgc ccgctcaatc tggtgctcaa caccgggcgg
    16201 catctggaaa ttcgcgtcat gccttatacc cacaaacagt tgctgatggt ggcgcgtgat
    16261 gtcacgcaaa tgcatcaact ggaaggggcg cggcgtaact tttttgccaa cgtgagccat
    16321 gagttacgta cgccattgac cgtgttacag ggttacctgg agatgatgaa tgagcagccg
    16381 ctggaaggcg cggtacgcga aaaagcgttg cacaccatgc gcgagcagac ccagcggatg
    16441 gaaggactgg tgaagcaatt gctgacgctg tcgaaaatag aagccgcacc gacgcatttg
    16501 ctcaatgaaa aggttgatgt gccgatgatg ctgcgcgttg ttgagcgcga ggctcagact
    16561 ctgagtcaga aaaaacagac atttaccttt gagatagata acggcctcaa ggtgtctggc
    16621 aacgaagatc agctacgcag tgcgatttcg aacctggtct ataacgccgt gaatcatacg
    16681 ccggaaggca cgcatatcac cgtacgctgg cagcgagtgc cgcacggtgc cgaatttagc
    16741 gttgaagata acggaccggg cattgcaccg gagcatattc cgcgcctgac cgagcgtttt
    16801 tatcgcgttg ataaagcgcg ttcccggcaa accggcggta gcggattagg gttagcgatc
    16861 gtgaaacatg ctgtgaatca tcacgaaagt cgcctgaata ttgagagtac agtaggaaaa
    16921 ggaacacgtt tcagttttgt tatcccggaa cgtttaattg ccaaaaacag cgattaatcc
    16981 gcctttgtca tcttttattg ccataagcca gtcgatgctg gcttattttc tttgcagtca
    17041 aaatacgggc gttagatttt acaacggttg gtgatttttt gttcgcatga ttagccatgt
    17101 ctttttcacg gaaatagtgt tttatactgg ttggtgattt cttatcgcta tatacctctg
    17161 gtttttagat ccctccttgc tttaaaacgt tataagcgtt taaattgcgc ttcaggtgct
    17221 gtcatactga ctgcattaac gcggtaaatc gaaaaactat tcttcgccgc gcctggttgg
    17281 gagtatttcc cgctaaaatt gtttaaatat accgctgtat catccccagg gattggcaca
    17341 aaaatttaac gttacaacac cacatccaca ggcagtatga tttatgaccc atcaattaag
    17401 atcgcgcgat atcatcgctc tgggctttat gacatttgcg ttgttcgtcg gcgcaggtaa
    17461 cattattttc cctccaatgg tcggcttaca ggcaggcgaa cacgtctgga ctgcggcatt
    17521 cggcttcctc attactgccg ttggcctgcc ggtattaacg gtagtggcgc tggcaaaagt
    17581 tggcggcggt gttgacagcc tcagcacgcc aatcggtaaa gtcgctggcg tactgctggc
    17641 aacggtttgt tacctggcgg tggggccgct tttcgctacg ccgcgtacag ctaccgtttc
    17701 ctttgaagtg gggattgcgc cgctgacggg tgattccgcg ctgccgctgt ttatctacag
    17761 cctggtctat ttcgctatcg ttattctggt ttcgctctat ccgggcaagc tgctggatac
    17821 cgtgggcaac ttccttgcgc cgctgaaaat tatcgcgctg gtcatcctgt ctgttgccgc
    17881 tattgtctgg ccggcgggtt ctatcagcac ggcgactgag gcttatcaaa acgctgcgtt
    17941 ttctaacggc ttcgttaacg gctatctgac catggatacg ctgggcgcaa tggtgtttgg
    18001 tatcgttatt gttaacgcgg cgcgttctcg tggcgttacc gaagcgcgtc tgctgacccg
    18061 ttataccgtc tgggctggcc tgatggcggg tgttggtctg actctgctgt acctggcgct
    18121 gttccgtctg gggtcagaca gcgcgtcgct ggtcgatcag tctgcaaacg gcgctgctat
    18181 tctgcatgct tacgttcagc acacctttgg cggcggcggt agcttcctgc tggcggcgtt
    18241 aatcttcatc gcctgcctgg taacggcagt tggcctgacc tgtgcttgtg cagaattctt
    18301 tgcccagtac gtaccgctct cttatcgtac gctggtgttt atcctcggcg gcttctcgat
    18361 ggtggtttct aacctcggct taagccagct gatccagatc tccgtaccgg tgctgaccgc
    18421 tatttatccg ccgtgtatcg cactggttgt attaagtttt acacgctcat ggtggcataa
    18481 ttcgtcccgc gtgattgctc cgccgatgtt tatcagcctg ctttttggta ttctcgacgg
    18541 gatcaaagca tctgcattca gcgatatctt accgtcctgg gcgcagcgtt taccgctggc
    18601 cgaacaaggt ctggcgtggt taatgccaac agtggtgatg gtggttctgg ccattatctg
    18661 ggatcgcgcg gcaggtcgtc aggtgacctc cagcgctcac taaatcactg aacatttgtt
    18721 ttaaccacgg ggctgcgatg ccccgtggtt ttttattgtg ttgatgggtt aggaattgat
    18781 ggaaagtaag aacaagctaa agcgtgggct aagtacccgc cacatacgct ttatggcact
    18841 gggttcagca attggcaccg ggctgtttta cggttcggca gacgccatca aaatggccgg
    18901 tccgagcgtg ttgttggcct atattatcgg tggtatcgcg gcgtatatca ttatgcgtgc
    18961 gctgggggaa atgtcggtac ataacccggc cgccagctct ttctcgcgtt atgcgcagga
    19021 aaacctcggc ccgctggcag gttacattac cggctggacc tactgctttg aaatccttat
    19081 tgtcgccatc gccgatgtga ccgcttttgg tatctatatg ggtgtctggt tcccgacggt
    19141 gccgcactgg atttgggtac tgagcgtggt gctgatcatt tgcgccgtaa acctgatgag
    19201 cgtgaaggta ttcggtgagc tggaattctg gttctcgttc tttaaagtcg ccaccatcat
    19261 catcatgatt gtcgccggtt tcggcatcat catctggggg attggcaacg gcgggcaacc
    19321 gaccggtatt cataacctgt ggagcaacgg cggcttcttc agtaacggct ggcttggcat
    19381 ggtaatgtcg ttgcaaatgg tgatgtttgc ttacggtggg atcgaaatta tcgggattac
    19441 cgccggtgaa gcgaaagatc ctgagaaatc gataccgcgt gcgattaact ccgtgccgat
    19501 gcgtattctg gtgttctacg tcggtacgct gttcgtcatt atgtctatct acccgtggaa
    19561 tcaggttggc actgccggta gcccgttcgt gctgacgttc cagcatatgg gcattacctt
    19621 tgccgccagc attcttaact ttgttgtgct gactgcttcg ctgtcggcaa ttaacagtga
    19681 tgtatttggc gtaggccgta tgctccacgg tatggcagag cagggcagcg cgccgaaaat
    19741 tttcagcaaa acgtcgcgtc gcggtattcc gtgggttacg gtgctggtga tgactaccgc
    19801 gctgctgttt gcggtgtatc tgaactacat catgccggaa aacgtcttcc tggtgatcgc
    19861 ttcgctggca accttcgcca cggtgtgggt gtggattatg atcctgctgt cgcaaattgc
    19921 cttccgtcgc cgtttgccgc cagaagaagt taaggcgctg aaatttaaag tgccgggtgg
    19981 ggtagcaacg accatcggcg ggctgatttt cctgctcttt attatcgggt tgattggtta
    20041 tcacccggat acgcgtatct cgctgtatgt cggtttcgcg tggattgttg tgctgttgat
    20101 tggctggatg tttaaacgcc gccacgatcg tcagctggct gaaaaccagt aatccttgcg
    20161 ttctgtaagc cggttcagga ggaaatcctg atccggctat tccgaaagtt atccgccccc
    20221 gtcctcctcc cccaaatatc cttcagatga tgagtgatcc tgcattaggc tatggcaagg
    20281 tgatcagatt ttcatcacag gggaattatg atgttaaatg catggcacct gccggtgccc
    20341 ccatttgtta aacaaagcaa agatcaactg ctcattacac tgtggctgac gggcgaagac
    20401 ccaccgcagc gcattatgct gcgtacagaa cacgataacg aagaaatgtc agtaccgatg
    20461 cataagcagc gcagtcagcc gcagccaggc gtcaccgcat ggcgggcggc gatcgatctc
    20521 tccagcggac aaccccggcg gcgttatagt ttcaaattgc tttggcacga tcgccagcgt
    20581 tggtttacac cgcagggctt cagccgaatg ccgccggcac gactggagca gtttgccgtt
    20641 gatgtaccgg atatcggccc acaatgggct gcggatcaga ttttttatca gatcttccct
    20701 gatcgtttcg cccgtagtct tcctcgtgaa gctgaacagg atcatgtcta ttaccatcac
    20761 gcagccggac aagagatcat attgcgtgac tgggatgaac cggtcacggc gcaggcgggc
    20821 ggatcaacgt tctatggcgg cgatctggac gggataagcg aaaaactgcc gtatctgaaa
    20881 aaacttggcg tgacggcgct gtatctcaat ccggtgttta aagctcccag cgtacataaa
    20941 tacgataccg aggattatcg ccatgtcgat ccgcagtttg gcggtgatgg ggcgttgctg
    21001 cgtttgcgac acaatacgca gcagctggga atgcggctgg tgctggacgg cgtgtttaac
    21061 cacagtggcg attcccatgc ctggtttgac aggcataatc gtggcacggg tggtgcttgt
    21121 cacaaccccg aatcgccctg gcgcgactgg tactcgttta gtgatgatgg cacggcgctc
    21181 gactggcttg gctatgccag cttgccgaag ctggattatc agtcggaaag tctggtgaat
    21241 gaaatttatc gcggggaaga cagtattgtc cgccactggc tgaaagcgcc gtggaatatg
    21301 gacggctggc ggctggatgt ggtgcatatg ctgggggagg cgggtggggc gcgcaataat
    21361 atgcagcacg ttgccgggat caccgaagcg gcgaaagaaa cccagccgga agcgtatatt
    21421 gtcggcgaac attttggcga tgcacggcaa tggttacagg ccgatgtgga agatgccgcc
    21481 atgaactatc gtggcttcac attcccgttg tggggatttc ttgccaatac cgatatctct
    21541 tacgatccgc agcaaattga tgcccaaacc tgtatggcct ggatggataa ttaccgcgca
    21601 gggctttctc atcaacaaca attacgtatg tttaatcagc tcgacagcca cgatactgcg
    21661 cgatttaaaa cgctgctcgg tcgggatatt gcgcgcctgc cgctggcggt ggtctggctg
    21721 ttcacctggc ctggtgtacc gtgcatttat tacggtgatg aagtaggact ggatggcaaa
    21781 aacgatccgt tttgccgtaa aacgttcccc tggcaggtgg aaaagcagga tacggcgtta
    21841 ttcgcgctgt accagcgaat gattgcgctg cgtaagaaaa gtcaggcgct acgtcatggc
    21901 ggctgtcagg tgctgtatgc ggaagataac gtggtggtat ttgtccgcgt gctgaatcag
    21961 caacgtgtac tggtggcaat caaccgtggc gaggcctgtg aagtggtgct acccgcgtca
    22021 ccgtttctca atgccgtgca atggcaatgc aaagaagggc atgggcaact gactgacggg
    22081 attctggctt tgcctgccat ttcggctacg gtatggatga actaacgttt tataacgcct
    22141 tgcgtgacgc ctgcgccatc atccgcggat aaaactgcca gaagcgggtt tcgagggcgt
    22201 cataatgagc gtctaaatcg taccaggagt cacgcagggc atccagacgt gggcggcggc
    22261 ttgccatgcc gtttaacacg ttctggatga aatccatatc gcgatagcgc accagccact
    22321 gctctgacca caagtaattg ttcagattga taaaacgtgg cggtgagtcc ggcaaaatcg
    22381 tcatcacttg ctcgcgggca taacagacaa attcctgtag cggaaagtcc ggcgacagct
    22441 gcgaccagtg gcgggaaaga aagtgatccc acatgacatc cagcgtaata ggcgcaacgc
    22501 ggcgcgtttc actacgaaac cactcccgtg cttcgcggac ttccggcaga ttgtcagtca
    22561 atacgtcgat acgtcgatgc atatgaatgc cagccacgac gtcgggcgga aaactttctt
    22621 caggatttcc gcgtacgaaa tcagccagta aattgccgga aagcgagctt tccgcgagat
    22681 gggctaaatg caggtgagct aaaaaattca tcgattatat tctatccaaa agggggtaaa
    22741 ggttgcaggg agagcgcccc ggcactagac tacccgcctc ttattttagt ctgagtcagt
    22801 gtcatgcgcg ttaccgattt ctcctttgaa ttgcccgaat ccctgattgc ccactatccc
    22861 atgcctgaac gcagtagctg tcgtttactg tcgctggacg ggccgacggg cgcgctgacg
    22921 cacggtactt tcaccgattt acttgataag ctcaaccccg gcgatcttct ggtttttaat
    22981 aatacccgcg tgatcccggc gcgcctgttt gggcgtaaag ccagcggcgg caagattgaa
    23041 gtgctggttg aacggatgct cgacgacaaa cgcattcttg cgcatattcg cgcctcgaaa
    23101 gcgccaaaac ctggcgcaga actgctgctg ggcgatgacg aaagtattaa cgcaacaatg
    23161 accgcgcgcc acggcgcact gtttgaagtc gaatttaatg atgaacgctc ggtgctggat
    23221 attctcaaca gcatcggcca tatgccgctg ccgccgtata tcgaccgtcc ggacgaagac
    23281 gctgaccgcg aactttatca aaccgtttat agcgaaaaac cgggcgcggt tgcagccccg
    23341 accgcaggtc tgcattttga cgagcctttg ctggaaaaat tgcgcgccaa aggcgtggag
    23401 atggcgtttg tgacgttgca cgttggtgcg ggcaccttcc agccggtgcg cgtcgacacc
    23461 attgaagatc acatcatgca ctcggaatac gctgaagtac cgcaggatgt ggtagacgcg
    23521 gtactggcgg cgaaagcgcg cggtaaccgg gtgattgcgg ttggcaccac ttcagtacgt
    23581 tcgctggaaa gcgcggctca ggcagcgaaa aacgatctca ttgaaccgtt cttcgacgat
    23641 acccaaatct ttatctatcc gggcttccag tacaaagtgg tcgatgcgct ggtgacgaac
    23701 ttccacttgc cagagtcgac gctgattatg ctggtttcgg cctttgccgg ttatcaacac
    23761 accatgaacg cctataaagc agcggtagaa gagaaatatc gcttttttag ttacggtgat
    23821 gcgatgttta tcacgtacaa tccgcaggca attaatgagc gcgtcgggga gtaattccgc
    23881 ggcgctggtt taaaacgttg gactgttttt ctgacgtagt ggagaaaaaa tgaaatttga
    23941 actggacacc accgacggtc gcgcacgccg tggccgcctg gtctttgatc gtggcgtagt
    24001 ggaaacgcct tgttttatgc ctgttggcac ctacggcacc gtaaaaggga tgacgccgga
    24061 agaagttgaa gccactggcg cgcaaattat cctcggcaac accttccacc tgtggctgcg
    24121 cccgggccag gaaatcatga aactgcacgg cgatctgcac gattttatgc agtggaaggg
    24181 gccgatcctc accgactccg gcggcttcca ggtcttcagc cttggcgata ttcgtaaaat
    24241 caccgaacag ggcgtgcact tccgtaaccc gatcaacggc gatccgattt tcctcgatcc
    24301 tgaaaaatca atggagattc agtacgatct tggttcggat atcgtcatga tctttgatga
    24361 gtgtacgccg tatcctgctg actgggatta cgcaaaacgc tccatggaga tgtctctgcg
    24421 ttgggcgaag cgtagccgtg agcgttttga cagtctcgga aacaaaaatg cgctgtttgg
    24481 tatcattcag ggcagcgttt acgaagattt acgtgatatt tctgttaaag gtctggtaga
    24541 tatcggtttt gatggctacg ctgtcggcgg tctggctgtg ggtgagccga aagcagatat
    24601 gcaccgcatt ctggagcatg tatgcccgca aattccggca gacaaaccgc gttacctgat
    24661 gggcgttggt aaaccagaag acctggttga aggcgtacgt cgtggtatcg atatgtttga
    24721 ctgcgtaatg ccaacccgca acgcccgaaa tggtcatttg ttcgtgaccg atggcgtggt
    24781 gaaaatccgc aatgcgaagt ataagagcga tactggccca ctcgatcctg agtgtgattg
    24841 ctacacctgt cgcaattatt cacgcgctta cttgcatcat cttgaccgtt gcaacgaaat
    24901 attaggcgcg cgactcaaca ccattcataa ccttcgttac taccagcgtt tgatggcggg
    24961 tttacgcaag gctattgaag agggtaaatt agagagcttc gtaactgatt tttaccagcg
    25021 tcaggggcga gaagtaccac ctttgaacgt tgattaatat taataatgag ggaaatttaa
    25081 tgagcttttt tatttctgat gcggtagcgg caacgggtgc accggcgcaa ggtagcccga
    25141 tgtctttgat tttgatgctg gtggtattcg gtctgatttt ctatttcatg atcctgcgtc
    25201 cacagcagaa gcgcaccaaa gaacacaaaa agctgatgga ctccattgcc aaaggtgatg
    25261 aagttctgac gaacggtggc ctggttggcc gcgtaaccaa agtagcggaa aacggctaca
    25321 ttgctatcgc gctgaatgac accactgaag tagttattaa acgtgacttc gtagctgccg
    25381 tcctgccgaa aggcaccatg aaggcgctgt aattaaaatt tttccctaag ggaattgccg
    25441 tgttaaaccg ttatcctttg tggaagtacg tcatgctgat cgtggtgatt gtcatcggtc
    25501 tgctgtatgc gcttcccaac ctgtttggtg aggatccggc tgttcagatc actggtgcgc
    25561 gcggagtcgc cgccagtgag caaacgctga tccaggtcca gaaaacgtta caagaagaaa
    25621 aaataactgc taagtctgtg gcactggaag agggcgctat tcttgcgcgc ttcgactcca
    25681 ctgacaccca gctgcgcgct cgtgaagcat taatgggcgt tatgggtgac aaatacgtcg
    25741 tggcgcttaa ccttgccccg gcaacgccgc gctggctggc agctattcac gctgagccga
    25801 tgaagctcgg ccttgacctg cgtggcggcg ttcacttcct gatggaagtg gatatggaca
    25861 ccgcgcttgg caaactccag gaacaaaata tcgatagcct gcgcagcgat ctgcgtgaaa
    25921 agggcatccc gtataccacc gttcgtaaag aaaacaacta cggcctgagc attactttcc
    25981 gcgatgctaa agctcgtgat gaagccattg cgtatctgag caagcgccat ccggacctgg
    26041 tgattagtag ccagggcagc aaccagctgc gtgcggtaat gagcgatgct cgtctgagtg
    26101 aagcgcgtga atatgcggtg cagcagaaca tcaatatcct gcgtaaccgt gtaaaccaac
    26161 ttggcgtggc ggagccggtg gttcagcgtc agggtgctga ccgtatcgtt gttgaactgc
    26221 caggtattca ggacactgcg cgtgcgaaag agattctggg tgcgacggca acgctggaat
    26281 tccgtctggt aaacaccaac gttgaccagg ccgctgcggc atccggtcgc gtaccgggcg
    26341 actctgaagt gaaacagacc cgcgaaggtc agccagttgt gctgtacaaa cgcgtaattc
    26401 tgaccggtga ccatatcacc gactccactt ccagccagga cgaatacaac caaccgcagg
    26461 ttaacatctc gctcgatagc gctggtggta acatcatgtc taacttcact aaggacaaca
    26521 tcggcaaacc gatggcaacc ctgtttgtgg agtacaaaga cagcggtaag aaagatgcaa
    26581 atggtcgtgc ggttctggtg aaacaggaag aggtgattaa catcgccaac atccagtctc
    26641 gtctgggtaa cagcttccgt atcaccggca tcaacaaccc gaacgaagcc cgtcagctgt
    26701 cactgctgct gcgtgccggt gcgttgatcg cgccgattca gattgttgaa gaacgtacca
    26761 ttggcccaac cctgggtatg cagaacattg aacaggggct ggaggcttgc cttgccggtc
    26821 tgctggtttc tattctgttc atgatcatct tctataagaa gtttggtctg attgcgacca
    26881 gtgctctgat tgccaacttg atcttaatcg tcggcattat gtcgctgttg ccaggcgcaa
    26941 cgctgagtat gccaggtatc gcgggtatcg tcttaaccct tgcggtggcg gtcgatgcga
    27001 acgtactgat caacgaacgt attaaagaag agttgagcaa cggacgtact gttcaacagg
    27061 caattgatga aggttatcgt ggcgcattca gttctatctt cgatgcgaac atcaccacgc
    27121 tgattaaagt catcatcctg tacgcagtgg gtaccggggc aattaaaggg ttcgcgatta
    27181 ctaccggtat cggtgtggcg acgtcgatgt ttaccgcgat tgtcggtacg cgtgccatcg
    27241 taaacctgct atatggcggc aagcgcgtca agaagctgtc aatctgagga gtgcgatgtg
    27301 gcacaggaat atactgttga acaactaaac cacggccgta aagtctatga ctttatgcgc
    27361 tgggactact gggctttcgg catctctggt ctgctgttaa tcgccgctat cgttattatg
    27421 ggcgtgcgcg gctttaactg ggggctggat ttcaccggtg gtacggttat cgaaattacg
    27481 ctcgaaaaac cggctgaaat tgacgtaatg cgtgatgcat tgcaaaaagc cggttttgaa
    27541 gagccaatgc tgcaaaactt tggtagcagc cacgacatca tggtccgtat gccgcctgct
    27601 gaaggcgaaa ccggcggtca ggtgctgggc agccaggtta tgaaggtgat taacgaatcc
    27661 accaatcaga atgcagcagt gaagcgtatt gagttcgtcg gtccgagcgt gggggcagac
    27721 cttgcgcaaa ccggtgcgat ggcgttgatg gcggcgctgc tgtctatcct tgtgtacgta
    27781 ggttttcgct ttgagtggcg actggcggca ggggttgtta ttgcgctggc gcacgacgtt
    27841 atcattacgc tgggtatttt gtcgttattc catatcgaga ttgacctgac cattgtggca
    27901 tcgttgatgt cggttatcgg ttactcgctt aacgacagta tcgtggtatc ggaccgtatt
    27961 cgtgaaaact tccgcaagat ccgtcgcggt acgccttacg aaatctttaa cgtgtccttg
    28021 acccagacgc tgcaccgtac cttgatcaca tccggtacta ccttgatggt tatcctgatg
    28081 ctgtacctct tcggtggtcc ggtactggaa ggcttctcgc tgaccatgct tatcggtgtt
    28141 tccatcggta ctgcatcttc catctatgtg gcatctgcgt tggctctgaa actgggtatg
    28201 aagcgcgaac acatgttgca gcagaaagtg gaaaaagaag gggcggatca gccgtcaatt
    28261 ctgccgtaat caagttcccg ttgatgttga aaatcccggt cagaagatcg ggattttttt
    28321 tgatgtatgg agattgcgaa aatccccgca tcttgggaaa ctgcgcgtaa ccctacattt
    28381 catccaggta actcttcatg gctatcatcc caaaaaacta tgcgcggtta gaaagcggct
    28441 atcgcgaaaa agcattaaaa atatatccgt gggtctgcgg tcgctgttcc cgcgagtttg
    28501 tttattccaa cctgcgtgaa cttactgttc accacattga tcacgaccat accaataacc
    28561 cggaagatgg cagtaactgg gaattgcttt gtctctattg ccacgatcat gagcattcga
    28621 aatataccga agcggatcag tatggtacga ccgttatcgc aggggaagat gcgcagaaag
    28681 atgtcggtga agcgaagtac aacccattcg ctgacctgaa agcgatgatg aacaagaaga
    28741 agtgattaaa acgtaaaatt gcctgatgcg ctacgcttat caggcctacg ttatttcagc
    28801 aatatattga atttacgtgc ttttgtgggg cggacaaagc gtttacgccg catccggcat
    28861 gaacaaagcg cacgttgtta acaatctgaa atgccgggaa taaatcccgg cattttcata
    28921 atcagaagtt gtaacctact accaggtaac caccccagcc ggtagagcga acgttgaagt
    28981 tgccgttgcc gaagttcagt tctgcatcgt cgttccactg accaccgtcg tgccagtaac
    29041 gagctacgac agagtagtgc cagtgatcgt agttcagagc cagaatatgg ctggaagcga
    29101 tagagttatt ggtacgggtc ttaataccgt tgattgcgtt accgctgtca tcccctaaat
    29161 cggaacccca gtcgaagttg gtgaagccga tgtagctcag ctgaccgccc cacagatcgg
    29221 taatcggcac aaagtattta attttgaaac ggtaaccgtc ccactcgttt tcgttcgctg
    29281 cgccatagtt ctgccactgg tatttcgcat agacgttcat ggacaggctc atcggcaggc
    29341 cagtgtcgat atcggtaccc agacccatgt accaggtgct ctggcgacca tctttattac
    29401 gacccatgtc gtaaatgtag ttgttcgcga agtaccactc tttgaacgga ccgaagctaa
    29461 ggtcagtatt agtcagcttg tcgatggaga aacgtggttc gatttccata aacagcggag
    29521 aaccgtggtt ccagatacct tttgcatcgg agttaccgcc gaagaatacc ggcgcatccg
    29581 cataaccata gaagtcgaac cagtcttttt tagcgaatgc ttcgtactca aggtaggtat
    29641 cgttgcggat ctgcggtccg aaacgggtgt gatagcttcc gacaacgtta acgctctggt
    29701 gccaccagtc ggaaagatac tgcggtttgt cgttttcagc tgcgttgaca gtaaaagacg
    29761 aagagagcgc cagtaccgca ccggctgcca gtaatgtttt tttcatatgt atgccactgt
    29821 ttgaaaatcc cttgcgggag tgaaaaaggc gcaaattgcg tttctaaata tttcgtatac
    29881 agaggagcct attataaaaa tcattgctca caaaaatatg tttcgtttca cagttctatc
    29941 atttacgtaa tcgattgcgt tcacgtttac acacattcgg gcagggattg tactgacttt
    30001 cacccttgtt gcaaactttt actatcaatc tgggtgatgt aagcggagta acaaaatgac
    30061 ggggaaagat gttgcgggac gcacaaaatc gccccgaaat acgtttactg cataacaggt
    30121 tgaatatcgt ggatacgtac gaagtctaac tgttctacag aaataccctt taactgtaac
    30181 ggaatatcaa catcgctggg ggcgaggacg ctggcagggg cattaatcaa ttgattctgc
    30241 acattgattt cctgaaagtt gtctattgtt ccctgaattt gcccatattc aacagttccg
    30301 ctgaatgcgg gcaagggatc attcgactcg ccctgaatac gtagcgtcag ggtggtgcca
    30361 tctgcatcag gcgtaatatt caccagcgac atacgtaaag taccgatctg gctttccagt
    30421 cttgccggtg ttttcgctcc tggcagaagg taaaccccac tgctggattt tgcattcagc
    30481 ctgttttgct gagtgatttt gaccgtttct ttattgagtt gcgtcatctc ttggttcagc
    30541 gtgctgacgc tgtgtttcat ctgtcgcact tcgctctgct gcacacaggc gctaaggctg
    30601 aacaggcttc ccagcaggag cagtcgaaaa acgttagtat tcataggtcg tccctttctc
    30661 gtcgcaatta gcctaatggt agagggtaaa agcagcgaaa gcatcgatcc tttatctcaa
    30721 aagcgttgcg cctttgttgt atcgtcagtt cagggtaaaa tagatttctg ttaaccacct
    30781 ggtcaggacg ccgtatgcat tgcccattct gtttcgccgt ggacactaag gtaattgact
    30841 ctcgtctcgt gggcgagggt tcatccgtac gccgccgtcg gcagtgtctg gtgtgtaatg
    30901 aacgtttcac cacctttgaa gtggcggagc tggttatgcc gcgtgttgta aaaagcaacg
    30961 acgtgcgtga accgtttaat gaagagaaat tgcgtagcgg aatgctgcgg gcgctggaaa
    31021 aacgtccggt gagttccgat gacgtcgaaa tggcaatcaa tcatattaaa tcgcaactgc
    31081 gcgccaccgg tgagcgcgaa gtgccgagca agatgattgg caatctggtg atggagcaat
    31141 tgaaaaagct cgataaagtc gcctatatcc gttttgcctc tgtctaccgc agtttcgaag
    31201 atatcaaaga atttggcgaa gagatcgcgc gcctggagga ctaagccgtg caggacgagt
    31261 attacatggc gcgggcgcta aagctggcgc aacgaggacg ttttaccacc caccccaacc
    31321 cgaatgtcgg gtgcgtcatt gtcaaagatg gcgaaattgt cggtgaaggt taccaccaac
    31381 gagcgggtga accacatgcc gaagtacacg cgttgcgtat ggcgggtgaa aaagccaaag
    31441 gcgcaacggc gtatgtgaca ctcgaaccct gtagccatca tggccgtacg ccaccgtgct
    31501 gtgacgcact catcgccgct ggcgtagcgc gcgtggtcgc agcgatgcag gatcctaacc
    31561 cgcaggtcgc tgggcgtgga ctttaccgtc tgcaacaggc tggcattgac gtcagccacg
    31621 ggctgatgat gagtgaagcc gagcaattga ataaaggctt tctcaagcgg atgcgcaccg
    31681 gctttcctta tattcagtta aaacttggcg catcgcttga tggccgcacg gcgatggcga
    31741 gcggcgaaag ccagtggatc acttcgcctc aggcgcggcg cgacgtacaa cgacagcgcg
    31801 cacaaagtca tgccatttta accagcagcg ccacggtgct ggcggatgat ccagccttaa
    31861 cagtgcgttg gtctgaactg gatgaacaaa ctcaggcgct ctacccgcaa caaaatctcc
    31921 gccagccagt acgtattgtg attgatagcc aaaatcgcgt gacgccggaa catcgcattg
    31981 tgcagcagcc cggcgaaacc tggttcgcgc gtacgcagga agattctagt gagtggccgg
    32041 aaacggtgcg taccttgctg attccagagc ataaaggtca tctggatctg gttgtactga
    32101 tgatgcaact gggtaaacag caaattaaca gcatctgggt ggaagcgggg ccaacgctcg
    32161 ctggcgcatt gctgcaggcg ggtttagtcg atgagctgat tgtctatatc gcacctaaac
    32221 tattaggcag cgacgcccgc ggattatgca cgctgccagg gcttgagaaa ttagccgacg
    32281 ccccccaatt taaattcaaa gagatacgtc atgtaggccc ggatgtttgc ctgcatttag
    32341 tgggtgcatg atctcccggc tcgaaaggga agcagcgcac gaaatattat gctaaaatcc
    32401 gcccccctgc ggggccatac tcgaacccga aggaagaaaa tgaacattat tgaagctaac
    32461 gttgctaccc cggacgctcg cgtcgccatc accatcgcgc gtttcaacaa ctttatcaat
    32521 gacagcctgc tggaaggtgc aattgacgca ctgaaacgta tcggtcaggt aaaagatgaa
    32581 aacattaccg ttgtttgggt gcctggtgcc tatgagctgc cgctggcggc gggtgcactg
    32641 gctaaaaccg gtaaatacga cgcggtgatt gcgctgggta cggttattcg tggtggcact
    32701 gcccactttg aatatgtcgc tggtggtgca agcaacggcc tggcgcatgt tgcccaggac
    32761 agcgaaattc cggttgcttt tggggttctg accactgaaa gcattgaaca agcgatcgaa
    32821 cgtgctggca ccaaagctgg caacaaaggt gcagaagctg cactgaccgc gcttgaaatg
    32881 attaatgtat tgaaagccat caaggcctga aattagtaag gggaaatccg tgaaacctgc
    32941 tgctcgtcgc cgcgctcgtg agtgtgccgt ccaggcgctc tactcctggc agttgtccca
    33001 gaacgacatc gctgatgttg aataccagtt cctggctgaa caggatgtaa aagacgttga
    33061 cgtcctgtac ttccgtgagc tgctggccgg ggtggcgact aataccgcat acctcgacgg
    33121 actgatgaag ccatacctgt cccgcctgct ggaagaactg ggacaggtag aaaaagcagt
    33181 actgcgcatt gcgctgtacg aactgtctaa acgtagcgat gtgccataca aagtggccat
    33241 taacgaagcg atcgaactgg cgaaatcgtt cggcgcagaa gacagccata agttcgtcaa
    33301 cggcgtactc gataaagcag cacctgtgat tcgccctaac aaaaagtgat atccaggccg
    33361 gtagattcac ggaagaccgt tccatgatcg ccggcctttt cttttttacc tgctgaggca
    33421 taacgtatgg catgtggcga gttctccctg attgcccgtt attttgaccg tgtaagaagt
    33481 tctcgtcttg atgtcgaact gggcatcggc gacgattgcg cacttctcaa tatccccgag
    33541 aaacagaccc tggcgatcag cactgatacg ctggtggcgg gtaaccattt cctccctgat
    33601 atcgatcctg ctgatctggc ttataaagca ctggcggtga acctaagcga tctggcagcg
    33661 atgggggccg atccggcctg gctgacgctg gcattaacct taccggacgt agacgaagcg
    33721 tggcttgagt ccttcagcga cagtttgttt gatcttctca attattacga tatgcaactc
    33781 attggcggcg ataccacgcg tgggccatta tcaatgacgt tgggtatcca cggctttgtt
    33841 ccgatgggac gagccttaac gcgctctggg gcgaaaccgg gtgactggat ctatgtgacc
    33901 ggtacaccgg gcgatagcgc cgccgggctg gcgattttgc aaaaccgttt gcaggttgcc
    33961 gatgctaaag atgctgacta cttgatcaaa cgtcatctcc gtccatcgcc gcgtatttta
    34021 caggggcagg cactgcgcga tctggcaaat tcagctatcg atctctctga cggtctgatt
    34081 tccgatctcg ggcatatcgt gaaagccagc gactgcggcg cacgtattga cctggcattg
    34141 ctgccgtttt ctgatgcgct ttctcgccat gttgaaccgg aacaggcgct gcgctgggcg
    34201 ctctctggcg gtgaagatta cgagttgtgt ttcacggtgc cggaactgaa ccgtggcgcg
    34261 ctggatgttg ctctcggaca ccttggcgta ccgtttacct gtatcgggca aatgaccgcc
    34321 gatatcgaag ggctttgttt tattcgtgac ggcgaacctg tcacgtttga ctggaaagga
    34381 tatgaccatt ttgccacgcc ataaagatgt cgcgaaaagt cgcctgaaga tgagtaatcc
    34441 gtggcatctg cttgctgtcg gattcggaag tggattaagc ccgatcgttc ctgggacgat
    34501 gggctcgctg gcagcgattc cgttctggta tctgatgacc tttttgccct ggcagctcta
    34561 ctcgctggtg gtgatgctgg ggatctgtat cggcgtctat ctttgtcatc agaccgcaaa
    34621 agatatgggc gtgcacgatc acggcagcat tgtctgggac gaatttattg gtatgtggat
    34681 cacgctcatg gcgctgccga ccaatgactg gcagtgggtt gccgccgggt ttgtgatttt
    34741 ccgtattctg gatatgtgga aaccgtggcc gatccgctgg tttgatcgca atgtgcatgg
    34801 cggcatgggg atcatgatag atgatattgt cgccggggtg atctccgctg gcatcctgta
    34861 ctttattggt catcactggc cgctgggcat tctgtcgtag ttgtgcaccg atgcctgatg
    34921 tgacgcttgt tgcgtctcat caggcctgga ctcttattta aatcctacta ccggatgcgg
    34981 tttatacggc gtttccagtt cggcaatctg ttccggcttc aaagtgatat ccaccgcgtt
    35041 caatagctca tcaagctgtt cttcccgcga tgtaccgata atcggtgcgg caatgcccgg
    35101 tttactcaac aaccaggcca gcgcaacttg tgctcgtgtt gcaccgagtt cttcactgac
    35161 gcccgttaac cgctctgcga tctgcgcgtc attttcatcg ctttctttat agagattttt
    35221 ccccacctca tcagacacca gtcgtgcggt agtttctccc cacggacgcg tcagtcgccc
    35281 ccgcgccagc gggctccacg gaatcaccgc cacgccctcc tgataacaca gtggcagcat
    35341 ctcgcgctct tcttcgcgat aaatcagatt gtagtgatcc tgcatactga caaactgcgc
    35401 ccagccgtgc tgtttttgga gttccagtgc ctgagcaaac tgcgaagcgt gcattgatga
    35461 cgcgccgata taacgcgctt tcccggcttt taccacgtcg ttcagggctt ccagcgtctc
    35521 ttcgatcggc gtgttgtaat cccagcgatg aatttgcagg atatcgacat aatccatgcc
    35581 gagacgtcgc aggctgtcgt cgatagagcg caatatttgc gcacgggata atccttccgg
    35641 caaatcacca acgcgatgga atactttggt ggcaacgacc acgtcttcac gacgggcgaa
    35701 atcccgcagt gcgcgaccga cgatctcttc gctgctgcca tcggaatagc tattggcggt
    35761 gtcaaagaaa tttatgccgc cttccagcgc gcgtttaatt atgggacggc tgctttcttc
    35821 cggcagtgtc catgcgtgat taccacgatc tggctcgcca aaggtcatac agccgaggca
    35881 aagtcgggaa acgcgaaggt cggtttttcc taaggggttg tattgcatgc tgccactcct
    35941 gccatactcg tcatacttca agttgcatgt gctgcggctg cattcgttca ccccagtcac
    36001 ttacttatgt aagctcctgg ggcttcactc gtttgccgcc ttcctgcaac tcgaattatt
    36061 tagagtctat gaataatttg ttaagcatag caggagtgga gtagggatta tgccagccag
    36121 gccttgattt tggcttccat accggcggca tcgaggccga gttcggcgcg catttcttcc
    36181 tgagttcctt gcggaataaa gaagtccggc agaccaatgt tcagcacggg tactggttta
    36241 cgatgggcca tcagcacttc gttcactccg ctacctgcgc cgcccataat ggcgttttct
    36301 tctacggtga ccagcgcttc atggctggcg gccatttcca gaattaacgc ttcatcaagc
    36361 ggtttcacaa aacgcatatc gaccagcgtg gcgttcagcg attcagcgac tttcgccgcg
    36421 tctggcatca gcgtaccaaa gttaaggatc gccagtttct cgccacgacg cttcacaatg
    36481 cctttgccaa ttggcagttt ttccagcggc gtcagctcca cgcctaccgc gttgccacgc
    36541 gggtagcgca ccgctgacgg gccatcgtta tagtgatagc cggtatagag catctggcga
    36601 cattcgtttt catcgctcgg ggtcataatg accatttccg gtatgcagcg caggtaagag
    36661 agatcaaaag caccctgatg ggtttgaccg tcagcaccaa caatgcccgc gcggtcgatg
    36721 gcgaacagga ccgggagttt ttgaattgcc acgtcatgca gcacctgatc ataggcgcgt
    36781 tgcaggaagg tggagtagat cgcgacaatg ggtttgtatc caccaatggc cagtcccgca
    36841 gcaaaggtca ctgcgtgttg ctcggcaatc gctacatcaa aatagcgatc cgggaattta
    36901 cgagaaaact cgaccatacc ggaaccttca cgcatcgccg gagtaatcgc catcagcttg
    36961 ttgtctttcg ctgccgtttc gcacaaccag tcgccaaaga tttttgaata gctcggcaaa
    37021 ccgccgctac ttttcggcaa acaaccgctg gagggatcaa atttaggcac ggcgtggaaa
    37081 gtgattgggt ctttttctgc cggttcataa ccacgacctt ttttggtcat gatatgcagg
    37141 aactgcgggc ctttcaggtc acgcatgttc ttcagcgtgg tgataagccc cagcacatcg
    37201 tgaccgtcaa ccgggccgat gtagttaaag cccagctctt caaacaacgt gccagggact
    37261 accatgcctt taatatgttc ttcggtacgt ttgagtagct ctttaatggg tggcacgcca
    37321 gagaaaactt ttttcccgcc ttcgcgcagt gaagagtaaa gcttaccgga aagcagctgc
    37381 gccagatggt tgttgagcgc gccgacattt tcggaaatcg acatttcgtt gtcgttgagg
    37441 accaccagca tatcaggacg gatatcgccc gcgtgattca tcgcttcaaa cgccatgcct
    37501 gcggtaatcg cgccatcgcc gattacacag acggtacggc gatttttgcc ttctttctcg
    37561 gcagcaaccg caataccaat tccggcactg atggaggttg atgaatgccc gacgcttaat
    37621 acgtcatatt cgctttcgcc gcgccacggg aacgggtgca ggccgccttt ctgacggatg
    37681 gtgccgattt tgtcgcggcg tccggtcaaa attttatgcg gataagcctg atgccccaca
    37741 tcccaaatca attggtcaaa cggggtgttg tagacatagt gcagcgccac ggtcagttcg
    37801 accgtgccca gcccggaggc gaagtgcccg ctggaacggc tcacgctgtc gagtaaatag
    37861 cggcgcagtt cgtcgcagag tttcggtaaa ctctctttcg gcaacagtcg taactcctgg
    37921 gtggagtcga ccagtgccag ggtcgggtat ttggcaatat caaaactcat caggggccta
    37981 ttaatactta ttgtttattt attacgctgg atgatgtagt ccgctagcgc ttccagtgcc
    38041 gaggtatcga gtgattgttc agccagttgt ttcagcgact gacgggcatc gtcgatcaga
    38101 tcccgggctt tcttccgggc ttgctcaaga cccagaagtg cagggtaggt acttttacca
    38161 agttgctggt cggcaccctg gcgttttccc aacgttgcag tatctcctac cacatccagg
    38221 atgtcatcct gaacctggaa ggcaaggccg atgctctctg cgtacttgtc gagtactggc
    38281 agagcacgac gccctttatc tccggcgctt aatgcgccaa ggcgaacggc ggcgcgaatc
    38341 aatgcgccgg ttttatgacg atgaatacgc tcaagcgcgt ccagaggtac gtgtttgcct
    38401 tccgcgtcta aatccaacgc ctgaccacca cacattccgg caataccgct ggcgctcgcc
    38461 agttcagaaa tcatcgaaat tctgtcgcga tccgacactt ccggcatatc agcatcgctt
    38521 aaaatcgaga acgccagcgt ttgtaaagcg tcgccagcga gaatcgcgtt cgcttcgcca
    38581 aacttcacat ggcaggtcgg caaaccgcga cgcagatcgt catcatccat cgccggtaaa
    38641 tcatcatgaa ttaatgagta agcgtggata cactctacgg cagcagcggg tgcgtccagc
    38701 gtgtttgtgc taacgccgaa catatgaccg gtggcataaa ccagaaaagg tcgcaggcgc
    38761 ttaccaccta ataatgcgcc atactgcatg gtttcgacca cgggagtgtt ctgaaagggc
    38821 agtggggcga taaaacggct cagcgcctgg ttggcctgct taacgcaggc ttcgagttgc
    38881 tgcggaaagt ccattactca ttgtccggtg taaaaggggt tagagaggcg tcttcattgt
    38941 cagacagcag aatttgtacg cgctgttcag cttgttgtaa tttggcctgc ccctgacgtg
    39001 ccagctgcac gccgcgttcg aactcgttca gcgcctcttc cagcggcagg tcgccacttt
    39061 ccagacgggt tacaatctgt tccagctcgc tcagcgcctt ttcaaagctg gcgggagcct
    39121 catttttctt cggcataatg aatgtctgac tctcaatatt tttcgccccg tcatggtaac
    39181 ggactcaggg caaatagcaa ataacgcgca atggtaaggt gatgtgcgca gcaaagcgat
    39241 gttagtggta tacttccgcg cctggatgca gccgcaggtg tgggctgctg tatttttccc
    39301 tatacaagtc gcttaaggct tgccaacgaa ccattgccgc catgaagttt atcattaaat
    39361 tgttcccgga aatcaccatc aaaagccaat ctgtgcgctt gcgctttata aaaatcctta
    39421 ccgggaacat tcgtaacgtt ttaaagcact atgatgagac gctcgctgtc gtccgccact
    39481 gggataacat cgaagttcgc gcaaaagatg aaaaccagcg tctggctatt cgcgacgccc
    39541 tgacccgtat tccgggtatc caccatattc tcgaagtcga agacgtgccg tttaccgaca
    39601 tgcacgatat tttcgagaaa gcgttggttc agtatcgcga tcagctggaa ggcaaaacct
    39661 tctgcgtacg cgtgaagcgc cgtggcaaac atgattttag ctcgattgat gtggaacgtt
    39721 acgtcggcgg cggtttaaat cagcatattg agtccgcgcg cgtgaagctg accaatccgg
    39781 aagtgactgt ccatctggaa gtggaagacg atcgtctcct gctgattaaa ggccgctacg
    39841 aaggtattgg cggtttcccg atcggcaccc aggaagatgt gctgtcgctc atttccggtg
    39901 gtttcgactc cggtgtttcc agttatatgt tgatgcgtcg cggctgccgc gtgcattact
    39961 gcttctttaa cctcggcggc gcggcgcatg aaattggcgt gcgtcaggtg gcgcattatc
    40021 tgtggaatcg ttttggcagc tcccaccgcg tgcgttttgt cgctattaat ttcgaaccgg
    40081 tcgtcgggga aattctcgag aaaatcgacg acggtcagat gggcgttatc ctcaaacgta
    40141 tgatggtgcg tgccgcgtct aaagtggctg aacgttacgg cgtacaggcg ctggtcaccg
    40201 gcgaagcgct cggccaggtg tccagtcaga cgctgaccaa cctgcgcctg attgataacg
    40261 tctctgatac gctgatcctg cgtccgctga tttcttacga caaagagcac atcatcaacc
    40321 tggcccgcca gattggcact gaagactttg ctcgcacgat gccggaatat tgtggcgtga
    40381 tctccaaaag cccgacggtg aaagcggtta aatcgaagat tgaagcggaa gaagagaagt
    40441 tcgacttcag cattctcgat aaagtggttg aggaggcgaa taacgttgat atccgcgaaa
    40501 tcgcccagca gaccgagcag gaagtggtgg aagtggaaac cgtcaatggc ttcggcccga
    40561 acgacgtgat cctcgatatc cgttctatcg atgaacagga agataagcca ctgaaagtcg
    40621 aagggattga tgtggtttct ctgccgttct ataaactgag caccaaattt ggcgatctcg
    40681 accagaacaa aacttggctg ctgtggtgtg agcgcggggt gatgagccgt ctgcaggcgc
    40741 tctatctgcg cgagcagggc tttaacaatg tgaaggtata tcgcccgtaa tttgtggttt
    40801 ttacgtcgca tctggtcaga tgcgacgttt gccgcatccg acactactcg taataattat
    40861 aaatccctgc cgccatcacc agttgtgatg ccacttcatg ggctttttca cgcccaacca
    40921 acaggtcgat aattttcaga ccaaagtcga tagctgtacc cggcccctgg ctggtcagca
    40981 attttacccg tgcatcccag acgacgcgct tgtccagcca ttgttcggcg ggaattttgt
    41041 ctttcagcgt cgggaagccg gtcatattac caatcgggaa gatatcgtgc ggcaccagca
    41101 cggtggctgg cgcggcgcaa atagccgcga cgatacgccc ggaacggtgg aactgtttaa
    41161 cggtttcaac cagcagagtg ctatcgcgaa aacactccgc gcctttaatg ccaccaggca
    41221 gcacgatcac gtcatattcg ccatcagcca cttcgaccag cggcgcatcc gccagcagct
    41281 tcacgccgcg cgagcaggta atcgccaggt taccatcgct ggcgacgctg gcagtggtga
    41341 ctttgatacc gccgcgaacc agcagatcga tagtggtgac ggcttcagtc tcttcactac
    41401 caggggcgag gcaaaccagt gccgatgcgc tcatattcac tctcctttct ttttaccatt
    41461 tcaaacaggc gggtgttttc cggtacggca atcccatgcg cgcgggcgcg gcgtaagaga
    41521 aaaccattga tatagtcgat ttcagtgtgg cgcagcgcgc ggatatcctg caacatcgac
    41581 gagatatttt ccgctgtggc atcaatcacc tgcatcacgt aatcacgcaa atcttctgct
    41641 gaagtatgat gcccttcgcg ttcgatcact gccgcgactt cttcgcatat ctgcataatt
    41701 tcttgcggat gatgacgtaa ttcaccgttc gggcaattcc agatggcagt cagtggatta
    41761 atcacacagt tgactgccag cttgcgccac agctcggcgc gaatattgtt atgccaggca
    41821 acgtcaggca acacggtttg caaaatatcc gccagataac tgtaatcgcc gtcctgttgc
    41881 cgtgctggac caatatgcgt gataccgttt gccacatgaa taatgacatt gccgtcgcgg
    41941 cgggctgcat gggtggtggt gcccatcagt aatggctgct gaatgttttg caactcttcg
    42001 atggtgccca tgccgttgtg aattaacagt attggcgtag ttacaggcag tgtggacgcg
    42061 aggcttttga cggcatcgga aacctgccat gctttcagcg tcaccaggag cagatcgctg
    42121 gtggcgagaa aatcgggatc gttggcggtc agcgattcgt taaatatcga accatctgtc
    42181 tcaaccagat tcacgctaca ataaggttgc ggtacgcgca gccagccctg aacttcatga
    42241 ccctgtttgc aaagtgctgt aagccataat tgccctaagg caccgcatcc caatacggta
    42301 attttcattg ttcctcctca cccgcaacca ctccgggtgt tcaataaggc tatcccttaa
    42361 ttgtgcatgc tgttgcgact atgcacaatt aagggatacg tcctggtgca ggactgtcgg
    42421 ttatttaact ttgcgggtat tatgcttcgc atcaaaaatg aagggagagg aaaagatgcc
    42481 atctttcgat attgtctctg aagttgatct tcaggaagca cgtaacgcgg tcgataacgc
    42541 gagccgcgaa gtggagtccc gttttgactt ccgtaacgtt gaagcctcat ttgagctgaa
    42601 cgacgccagc aaaaccatca aagtgttgag cgagtccgat ttccaggtca atcagttgct
    42661 ggatattctg cgtgccaagc tgctgaagcg cggcattgaa ggcagttcgc tggatgtacc
    42721 ggagaatatc gttcatagcg gtaaaacctg gtttgtggaa gcgaaactga aacagggcat
    42781 tgagagcgcg acccagaaga aaatcgtcaa gatgatcaaa gacagcaaac tgaaagtgca
    42841 ggcgcaaatt cagggcgatg aaatccgcgt aacgggcaaa tctcgtgatg atttgcaggc
    42901 tgtcatggcg atggtacgtg gtggcgatct cggtcagccg ttccagttca aaaacttccg
    42961 cgattaatcg cgactatgtg cgttttgttc atgtcagatg cggcatgaac gcctgattcg
    43021 gcctacaaag gtttgtaatt cagaaacttt gtaggcctga taagcgtagc gcagcaggca
    43081 attcgccgcg ttttttatgc ctgacgaatt gcctgttcta tctcaaagcg attcgtcact
    43141 ttgctgtcga ttttcacata agctgaatgt tcttcttctg caatcaacac ttctttgatg
    43201 ccttcagttt ctagcaaacg cacttttaac gcctcgtttg cggcaatgtt cgccgggatt
    43261 tcaatgcgca aactgctgac atacggcggt tctttcatgg tactggcgac tgtcagccac
    43321 actgcggcca gcattgcgcc agcgagaaat accccctgac cgtcaaacat gccgtcaatc
    43381 cagccgccca gcgaaccgcc aatcgccacg ccaagaaact ggctggtgga gtaaacaccc
    43441 atcgccgtac ctttgtaacc tgctggcgac tctttactga taagtgaggg caggagggct
    43501 tccatcaaat taaacgccac aaagaaaagc tgcacgccga ccaccagttg ccagaactgc
    43561 gtttgcgcgt tccacaacac aatttccgca accacgatca acccgacgca gaagacaaag
    43621 acttgcttca ttttgcgctt aacttcagcg tagataatga aaggcacgac cgagccaaag
    43681 gcgattagca ttgtcgccag atagaccttc cagtgttcag ccgccgggaa ccctgcatca
    43741 gccagttgtc cgggcagggc aacaaacgtc gacatcagca aaatatgcag acacataatg
    43801 ccaaagttga gtttcagcag ccgcggttcc gccagcactt tactgaaact gcctttcacc
    43861 attccggact cacgattaag tacgtgagtg ctactgttgg gcacaaccca aatggtcaac
    43921 gcaatgccgg tcgttgccag aatagcgatc atccagaaca gcgcgtgcag cccaagtttg
    43981 tgagtgatga tcgggccaag caccatcgca atggcaaagg taatgccaaa gctcacgccg
    44041 ataaacgcca ttgctttggt gcggttttgt tcgcgcgtga gatcggaaag cagcgccata
    44101 acggcagcgg caatcgcacc ggagccttgt agcgcccggc ccagaataat tccccagatg
    44161 gagtcagaaa gcgcagcgat aacgctaccg gcggcaaaca ccgtcagccc accgacaatt
    44221 aatggtttgc gaccaatgcg gtcggaaagc aggccaaacg gaatctgaaa aacggcctga
    44281 gtcagaccat aaataccaat ggcaataccg attaatgctt cgctggcacc ttgcagagcc
    44341 atgccatacg tggtcagaac cggcagaacc ataaacatgc ccagcatgcg caacgagaaa
    44401 acggttccta aaccccaggt cgcgcgccgc tcacctggcg tcattttata atcgttcatt
    44461 accacctctg ttttaaattc gcgactagtg taaagcggca aacgaatggg gtaaacatgc
    44521 ggatttttta gtaaatattt cgagagggat atgtttctaa tgctaagaaa aaaggtgccg
    44581 tagcaccttt ttaactgaga ggttttgtta ccacacagca gccagcagcg tatgcgagtc
    44641 cggtaccata aaatcaacgg acatcatcac cgagagggca gtgatggcga tgatagagaa
    44701 gccgaacagc ttgcgcgccc agattctgtc atcagcaact ttataaccgc gcagagccat
    44761 acctaaccac cagacgctaa ccgccgcggc gaccaccaga tatttatatc cagcgtaacc
    44821 gccaagagag agcatcagcg tggcaacggc aaaggcgatg atatacagcg tgatgtgatt
    44881 cttcgccacc gaaatgcctt ttaccactgg caataccgga atgtttgccg cctggtaatc
    44941 cttaaagcgg aaaatggcga tggcatagga gtgaggcatc tgccacaggc tgaagatagc
    45001 cagcaggatc gctgcgccgc tatcgaactc gccggttact gcacagtagc caatcaccgg
    45061 cggcgcagcg ccggagagcg aaccaatcaa tgtgccgtag actgagtggc gtttcatgta
    45121 caggctatag acgccgacat aaaccacaaa gcccatcacc cccagccagc aggccagcgg
    45181 attcgcgcca aaccacagca gcataaagcc agcaataccc agcaaggtgg cgtacaccag
    45241 cgagacagca ggagagatca ggcctttaac cagcacccga ttcttcgtcc tttccatctt
    45301 tctgtcgata tccctgtcga tgtagttgtt aaacacacaa cccgacgcca caaccagtga
    45361 caccccaacc agcgtgtaga taaacaaggg ataatcaatg ctgccctttg aggccagcag
    45421 gaatccccca atcaccgaga tcaggttgcc aaagatgatg cctggtttcg ttacttgcag
    45481 gtattgctta aacatcataa ccgccgctct tagtgcatca tcatgttgta gttgaggttc
    45541 cacataatcc agatggagcc tacaaccagg atagcgatga ttagcacggt gaagacaaac
    45601 gccgtcatgt tccagccttc atctgatttg gtattcatgt gcaggaagca caccagatgc
    45661 accagaacct gtaccactgc cattgccagg attgttccca gaattacggc cggagaggca
    45721 gcccctgtca tcaccatcca gaacggaatc accgtcagaa tgatcgacag gataaagcct
    45781 gtcatgtagg tttttacgct gccatgggac gcgccgctgt gatcggtaga atgactcatt
    45841 acatcgcccc catcagataa acaacagtga acacacagat ccaaaccaca tccaggaagt
    45901 gccagaacag gctcaggcac atgatgcggg tacggttagt gctggtcagg ccgcgacgag
    45961 cgatttgtac catcagcacc gccatccaga taagaccgga agtgacgtgc agaccgtgcg
    46021 tgccgaccag cgcaaagaac gctgacagga agccgctgcg atccggaccc atgccgttaa
    46081 caatcaggtg atggaattcg tagatttcca tcccgataaa tccggcacca aacaaccagg
    46141 tcaacgccag ccaggagata acctggcttt tgttgttttt gtacatggcg atagccgcca
    46201 tgccgtaggt gatggagctg aacaacagca agaaagtttc aaccagaacg aacggcagtt
    46261 cgaaaatgtc cttacctgtc gggccgcctg cggtgccgtt caccagaacg gcataggtag
    46321 caaacaagat tgagaacaga atgcagtcgc tcatcaggta gatccaaaat ccgaagattt
    46381 tggttccgcc tgcatcgtgg tgcccgtgtt cgtgcgcgtg ggcagtcgcg tgcgtcaaag
    46441 tatcagttgc catttttcag ccctgcctta gtaatctcat cgaaatgctg gttttccagt
    46501 ttttcgattt ctgccaccgg cacgtagtaa tccacgtcct cgtcgaagct tttcacgatc
    46561 caggtgatga tcatgcctgc gaagccaaca atcgccagcc accagatatg ccagatcatg
    46621 gcgaaaccga agatggtgga gaaagctgca atgacgatac ctgcaccgct gtttttcggc
    46681 atatgaattt cttcatagtg gtcaggcttt ttatacgctt cgcctttctc tttcatttcc
    46741 cagaatgcat cacgctcgtg aacgtgcggc acaacggcaa agttatagaa cggaggcggg
    46801 gaagaggttg cccactccag cgtacggcca ccccacgggt cgccagtcag gtcacggttc
    46861 tggtcgcggt cgcgaataga aacgtacatc tgaataacga ggcagagaat acccagcgca
    46921 atcagtactg caccgctggc tgcaatcatc agcatggtgt ggaactgcgg gtcaatctgc
    46981 tggctcaaac gacgggtcat gcccatgaag cccagcgcat acagcggcat aaaggcaacg
    47041 aagaagccga tgatccagaa ccagaacgcg cgtttacccc aggtttcgtt cagtttgaaa
    47101 ccgaacgctt taggccacca gtaggtcatc cctgcgaagc agccgaagac cacgccgccg
    47161 atgatcacgt tatggaagtg cgcaatcagg aacaggctgt tatgcagaac gaagtccgcg
    47221 cccggtactg ccagcagcac gccagtcatc ccgcccaccg agaaagtgac gataaaaccg
    47281 atggtccaca gcatcgcaga atggaacacg atgcggccct gatacatggt gaacagccag
    47341 ttgaagatct tcaccccggt cgggatggcg ataatcattg tggtgatacc aaagaaggcg
    47401 tttacgttcg cgcccgcacc catcgtaaag aagtggtgca gccaaacgat gaacgacagc
    47461 acggtgatac agacggttgc ccataccagc gaggtataac caaacagacg tttacgcgag
    47521 aaggttgccg caatttcgga gaacacaccg aaaacaggca ggatcaggat gtaaacttcc
    47581 gggtggcccc aggcccaaat caggttgatg tacatcatca tgttgccacc catatcgttg
    47641 gtaaagaaat gggtgcccag atagcgatcc agggtcaaca acgcgacggt aaccgtcaga
    47701 attgggaagg aagcaataat cagtacgttc gcgcacagtg atgcccaggt aaatactggc
    47761 atcttgaaca tggtcatgcc cggtgcgcgc atcttcagaa tggtaacgaa gaagttgata
    47821 ccggtaagcg tcgtacctat accggatagc tggagactcc atatccagta atcgacaccg
    47881 actcccggac tgtactctat tcccgatagc ggtggatagg ccagccagcc ggtctgcgca
    47941 aattcgccca cgccgagaga aacgttaacc agaatcacac caacaacagt aaaccagaag
    48001 cttaagttgt tgaggaacgg gaacgcaacg tcacgcgcgc cgatctgcag cggaaccacc
    48061 aggttcatca gaccgataac gaaaggcatc gccacgaaga agatcataat cacgccgtgc
    48121 gcggtgaaga tctgatcgta gtggtgaggt ggcaggaagc ccgcttcgcc cgccgaggca
    48181 agagcctgct ggctacgcat cataatggcg tcagcaaaac cacgcagcaa catcacaatc
    48241 gccacgatga tatacatgat accgaggcgt ttatggtcga cggaggtcag ccactctttc
    48301 cacaggtagg tccacttacc gaagtaactg atcaggccaa cgagcgccag acctcccaaa
    48361 ataatgccag cgatcgtaac catgacgata ggttcatgga acgggactgc atcaagtgat
    48421 aattttccga acatctttat tcttcctcaa cccctttaat gggcggattc cgcgtggctc
    48481 atgtccatgc cttccatacc ttcgtgtgca ctgtgctcac cttctggctg ggtcatgtcc
    48541 atgctcttac cgtgagccat aaacttgtta atcacatcgg caaacaagtc tggtttcacg
    48601 ttggagaaat attccacctg gttgtattcg ctaggcgcgg ccagtttttc gaacgcagcc
    48661 atgtcagaca tggtgttcgg cgactgtttc gcttttgcga cccattggtc gaattcggcg
    48721 cgatccggtg ttgcaatagc tttgaacttc gtgcctgaga agcccggccc gctgtagctg
    48781 gcggagatac cgtcataagt accgggttcg ttggcgatca gatgcaggcg agtctgcata
    48841 ccggccatgg cataaatctg gctacccaga cgcggaatga agaaggagtt catcacggag
    48901 ttggaggtca ctttgaagta caccggagtg ttcgccggga aagcgatttc attcacggta
    48961 gcaatgccct gttccgggta gatgaagaac catttccagt ccatggaaac cacttcgatg
    49021 gtaatgggct tctcgtcgtg tgccagcggc ttgctaggct caagagcgtg agtggttttc
    49081 caggtcagta ccgcaaggaa gatgatgatt aagataggta ccgtccagac cacagcttcc
    49141 actttattgg agtgtgacca gttcgggctg tacttagcat ctttattgct cgcacgatac
    49201 ttccaggcga aaccaacagc catcaagatt gcgggaataa cgacaatcaa catcaggcca
    49261 aatgccgtca gtatcagtga acgttgctcc agaccaatct gtcctttggg atctaacagc
    49321 gcagaattac agccactgag caatacagtg cctgcaaata atgacaacca tcccaaactt
    49381 ttattgtatt tcctgagtct catttaacga cctcaattcc acgggatctg gtggcgttta
    49441 aagtgtgtgg gcattttacg ggaaggttac attactgtaa acatgattaa attcctgtta
    49501 cttggatttg gctgtcaggt cacatatgtt gcaaaacata tcaagttgat tttaagaagc
    49561 tgttgcaaaa gggcgttata acgaaagggg agtgcagaag gagtttggac gggcaattgg
    49621 tataaccaat ctggaataaa acaattgttt aacaaataat tatcattatg gtgacaattg
    49681 ggtgggggga aaataaagac tcattagctg aatcgtgtaa gaaaaaatag aattcataaa
    49741 ttgcgtcaat aatttccaaa cgctaagccg cacaaaagaa caaatattat taagcgatac
    49801 tgatgtttat ttagccgtaa taattacggc gagtgattac tacagctaaa taatatttac
    49861 agattacgtc agatgcgttt ttcgtagcgc cagataatcc agcaaaccac caagcacgac
    49921 gccagaaagg gcgactaaaa ccccgacttc cagcagagca ggcaggaaag agaagtgcgt
    49981 caaatccagc gcgtccatcg tcaacagcag taaccacacg gccaacaggc tgacgcccgc
    50041 cgccagtgtc cacatggcaa aggcataacc tgccggatat tcggtacggg agataaagtt
    50101 gtcatttact cgtgtatatt caagcgtctg gcggcaaacc agcagcaaaa taagccccgg
    50161 tacggcagcg gcgacggaga atagatagaa tgtcgaccag ccgtgtgctt caacaaacca
    50221 acccgccacg gggccgacat aaactcgtcc tacagcggaa agcgctgaga gcagggcaaa
    50281 ttgagttgcg gaaaatgact tattacacag cgtcattaac agcgcgacaa aggctgatgt
    50341 gcccatcccg ccacagaggt tttcgaaaaa gacggctgcg cccatgctgt agagatgctt
    50401 atcagtaatc gacagcagcc agtaaccagc gttagacgca ccttgtaaaa tgccgaaaat
    50461 cagcagcgcc cggaacagcg acaggcgctg catcaaaatc ccaccgtaca atgcgcccac
    50521 aatggtcgcc agtaagccaa gcgttttgtt aaccacgcct acttcacccg catcaaaccc
    50581 gacgccgcga atcaaaaacg tggttgtcag gctcatggcg aatgcgtcgc ccagcttata
    50641 cagcacgata agaagcaaaa taagccaggc attattgcga ccaaagaaat ctcgcagagg
    50701 tgcaacaacc gcttgttcca gcgtttttgg cacaggaatg gtgtcggtcg gttctggtgc
    50761 aagcaacgtc gcgataatac aagggatcaa cagcgccgcc atcaaccagt acatgccctg
    50821 ccagcccagc catttatctg ccagccacag ggctaagccg ccggaaacca gcatccctaa
    50881 acggtaaccc agcacgctga ttgccgcgcc cgcaccacgt tcttctgccg ggagcacatc
    50941 ggttttccac gcatcgaaga caatatcctg agaggccgag caaaaagcga tcaccaccgc
    51001 cagcgccgcc atccagcgga gttgggtgcc tggttcgaga aaacccatcg ccgcaatggc
    51061 gactaataac aggatttgcg tggcgagcag ccaaccgcgc cgccgcccaa aaaatggggg
    51121 cgtgtagcgg tccatcagcg gtgaccagag gaatttaaaa acgtaagcct ggcctaccag
    51181 agagaagaaa ccaatggttt tgagatcaat attctcgacc gtcatccagg cctgtaaggt
    51241 accggatgtc agggcgagcg gtagcccgga agcaaaaccc aggatcagca atatggctga
    51301 acgcggctgt tgaaaaatac gtaaatattg actggacatg ggcgtctaca ggcccggctt
    51361 gcaccgggcc agagggcaga aattaacgcg cgttctgctt gatgaattcg tggatgctgg
    51421 tgtcctgaga catatcagcg atggtatcgg tcagcacact gttaaccgca tcggcgatat
    51481 ttttgttgga ggcctggaac gcaccttcaa cgttgtagct ggcacgatag tttttggtca
    51541 ttttgttgcc attctgcgcg gtagcgatga tggcgatatc cgctttggtc gcgatgttgt
    51601 agcgcacgtt gccctgggac acgtcagcat acagttggct aacgatgatt tgcagattaa
    51661 ccgggccatt cggaccaacc atgtaaccac gcgcggtcat ctgtttttcc agcacttctt
    51721 gcagcaggaa acgcagatcg cgggaggcgg tcagggtaac gatttgatta tcgcgggtga
    51781 cttttgccag cgcctgatcg gtacgctgat cggcaccatt aatgcttacg gtgacgccca
    51841 tcaggcttgg atcctgctgt ggcagtgtaa tcgtcgggga aacttcaata gttgttggcg
    51901 gttttgcgca tcctgcaagc ataaacagag caactaacgg gaagaggatt tttttgaaca
    51961 tgttcgggct ctcagagact cttaagcgtg tttggtaaaa attcccgcca tcataacatt
    52021 gccaacggcg aggggaagtg ggtaaggcat gtaaattcat catgttgacg aaataatcgc
    52081 ccctggtaaa agaaacactg atgcgaggcc tgtgtttcaa tctttaaatc agtaaacttc
    52141 atacgcttga cggaaaaacc aggacgaaac ctaaatattt gttgttaagc tgcaatggaa
    52201 acggtaaaag cggctagtat ttaaagggat ggatgacatc tcagcgttgt cggaggagat
    52261 atttcatgat gatacgtgag cggatagaag aaaaattaag ggcggcgttc caacccgtat
    52321 tcctcgaagt agtggatgaa agctatcgtc acaatgtccc agccggctct gaaagccatt
    52381 ttaaagttgt gctggtcagc gatcgtttta cgggtgaacg ttttctgaat cgtcatcgaa
    52441 tgatttacag tactttagcg gaggaactct ctactaccgt tcatgcgctg gctctgcata
    52501 cttacactat taaggagtgg gaagggttgc aggacaccgt ctttgcctct cctccctgtc
    52561 gtggagcagg aagcatcgcg taaaaacgca tttgcaactg tcggcgcttt tccagtatgt
    52621 tgctaaagat tttatgaaaa acggcctgcg ggccgttttg ttttgtctgg attttgcgct
    52681 ttttgcccag cattcagacg aaaattgccc gggaattgtg aaaaaatacg cgacagcgcg
    52741 caataaccgt tctcgactca taaaagtgat gccgctataa tgccgcgtcc tatttgaatg
    52801 ctttcgggat gattctggta acagggaatg tgattgatta taagaacatc ccggttccgc
    52861 gaagccaaca acctgtgctt gcggggtaag agttgaccga gcactgtgat tttttgaggt
    52921 aacaagatgc aagtttcagt tgaaaccact caaggccttg gccgccgtgt aacgattact
    52981 atcgctgctg acagcatcga gaccgctgtt aaaagcgagc tggtcaacgt tgcgaaaaaa
    53041 gtacgtattg acggcttccg caaaggcaaa gtgccaatga atatcgttgc tcagcgttat
    53101 ggcgcgtctg tacgccagga cgttctgggt gacctgatga gccgtaactt cattgacgcc
    53161 atcattaaag aaaaaatcaa tccggctggc gcaccgactt atgttccggg cgaatacaag
    53221 ctgggtgaag acttcactta ctctgtagag tttgaagttt atccggaagt tgaactgcag
    53281 ggtctggaag cgatcgaagt tgaaaaaccg atcgttgaag tgaccgacgc tgacgttgac
    53341 ggcatgctgg atactctgcg taaacagcag gcgacctgga aagaaaaaga cggcgctgtt
    53401 gaagcagaag accgcgtaac catcgacttc accggttctg tagacggcga agagttcgaa
    53461 ggcggtaaag cgtctgattt cgtactggcg atgggccagg gtcgtatgat cccgggcttt
    53521 gaagacggta tcaaaggcca caaagctggc gaagagttca ccatcgacgt gaccttcccg
    53581 gaagaatacc acgcagaaaa cctgaaaggt aaagcagcga aattcgctat caacctgaag
    53641 aaagttgaag agcgtgaact gccggaactg actgcagaat tcatcaaacg tttcggcgtt
    53701 gaagatggtt ccgtagaagg tctgcgcgct gaagtgcgta aaaacatgga gcgcgagctg
    53761 aagagcgcca tccgtaaccg cgttaagtct caggcgatcg aaggtctggt aaaagctaac
    53821 gacatcgacg taccggctgc gctgatcgac agcgaaatcg acgttctgcg tcgccaggct
    53881 gcacagcgtt tcggtggcaa cgaaaaacaa gctctggaac tgccgcgcga actgttcgaa
    53941 gaacaggcta aacgccgcgt agttgttggc ctgctgctgg gcgaagttat ccgcaccaac
    54001 gagctgaaag ctgacgaaga gcgcgtgaaa ggcctgatcg aagagatggc ttctgcgtac
    54061 gaagatccga aagaagttat cgagttctac agcaaaaaca aagaactgat ggacaacatg
    54121 cgcaatgttg ctctggaaga acaggctgtt gaagctgtac tggcgaaagc gaaagtgact
    54181 gaaaaagaaa ccactttcaa cgagctgatg aaccagcagg cgtaatttac gcagcataac
    54241 gcgctaaatt cgcacaaagg cccgtcaccg ccaggtggtg ggcttttttt tgtcatgaat
    54301 tttgcatgga accgtgcgaa aagcctcttt cggtgttagc gtaacaacaa aagattgtta
    54361 tgcttgaaat atggtgatgc cgtacccata acacagggac tagctgataa tccgtccata
    54421 aggttacaat cggtacagca ggttttttca attttatcca ggagacggaa atgtcataca
    54481 gcggcgaacg agataacttt gcaccccata tggcgctggt gccgatggtc attgaacaga
    54541 cctcacgcgg tgagcgctct tttgatatct attctcgtct acttaaggaa cgcgtcattt
    54601 ttctgactgg ccaggttgaa gaccacatgg ctaacctgat tgtggcgcag atgctgttcc
    54661 tggaagcgga aaacccagaa aaagatatct atctgtacat taactcccca ggcggggtga
    54721 tcactgccgg gatgtctatc tatgacacca tgcagtttat caagcctgat gtcagcacca
    54781 tctgtatggg ccaggcggcc tcgatgggcg ctttcttgct gaccgcaggg gcaaaaggta
    54841 aacgtttttg cctgccgaat tcgcgcgtga tgattcacca accgttgggc ggctaccagg
    54901 gccaggcgac cgatatcgaa attcatgccc gtgaaattct gaaagttaaa gggcgcatga
    54961 atgaacttat ggcgcttcat acgggtcaat cattagaaca gattgaacgt gataccgagc
    55021 gcgatcgctt cctttccgcc cctgaagcgg tggaatacgg tctggtcgat tcgattctga
    55081 cccatcgtaa ttgatgccag aggcgcaact gtgccgctat acttatccag ggcggcacaa
    55141 cgctgtaagc ggcttgcgcc tgagaatggc atttgcgtcg tcgtgtgcgg cacaaagaac
    55201 aaagaagagg ttttgaccca tgacagataa acgcaaagat ggctcaggca aattgctgta
    55261 ttgctctttt tgcggcaaaa gccagcatga agtgcgcaag ctgattgccg gtccatccgt
    55321 gtatatctgc gacgaatgtg ttgatttatg taacgacatc attcgcgaag agattaaaga
    55381 agttgcaccg catcgtgaac gcagtgcgct accgacgccg catgaaattc gcaaccacct
    55441 ggacgattac gttatcggcc aggaacaggc gaaaaaagtg ctggcggtcg cggtatacaa
    55501 ccattacaaa cgtctgcgca acggcgatac cagcaatggc gtcgagttgg gcaaaagtaa
    55561 cattctgctg atcggtccga ccggttccgg taaaacgctg ctggctgaaa cgctggcgcg
    55621 cctgctggat gttccgttca ccatggccga cgcgactaca ctgaccgaag ccggttatgt
    55681 gggtgaagac gttgaaaaca tcattcagaa gctgttgcag aaatgcgact acgatgtcca
    55741 gaaagcacag cgtggtattg tctacatcga tgaaatcgac aagatttctc gtaagtcaga
    55801 caacccgtcc attacccgag acgtttccgg tgaaggcgta cagcaggcac tgttgaaact
    55861 gatcgaaggt acggtagctg ctgttccacc gcaaggtggg cgtaaacatc cgcagcagga
    55921 attcttgcag gttgatacct ctaagatcct gtttatttgt ggcggtgcgt ttgccggtct
    55981 ggataaagtg atttcccatc gtgtagaaac cggctccggc attggttttg gcgcgacggt
    56041 aaaagcgaag tccgacaaag caagcgaagg cgagctgctg gcgcaggttg aaccggaaga
    56101 tctgatcaag tttggtctga tccctgagtt cattggtcgt ctgccggttg tcgcaacgtt
    56161 gaatgaactg agcgaagaag ctctgattca gatcctcaaa gagccgaaaa acgccctgac
    56221 caagcagtat caggcgctgt ttaatctgga aggcgtggat ctggaattcc gtgacgaggc
    56281 gctggatgct atcgctaaga aagcgatggc gcgtaaaacc ggtgcccgtg gcctgcgttc
    56341 catcgtagaa gccgcactgc tcgataccat gtacgatctg ccgtccatgg aagacgtcga
    56401 aaaagtggtt atcgacgagt cggtaattga tggtcaaagc aaaccgttgc tgatttatgg
    56461 caagccggaa gcgcaacagg catctggtga ataattaacc attcccatac aattagttaa
    56521 ccaaaaaggg gggattttat ctccccttta atttttcctc tattctcggc gttgaatgtg
    56581 ggggaaacat ccccatatac tgacgtacat gttaatagat ggcgtgaagc acagtcgtgt
    56641 catctgatta cctggcggaa attaaactaa gagagagctc tatgaatcct gagcgttctg
    56701 aacgcattga aatccccgta ttgccgctgc gcgatgtggt ggtttatccg cacatggtca
    56761 tccccttatt tgtcgggcgg gaaaaatcta tccgttgtct ggaagcggcg atggaccatg
    56821 ataaaaaaat tatgctggtc gcgcagaaag aagcttcaac ggatgagccg ggtgtaaacg
    56881 atcttttcac cgtcgggacc gtggcctcta tattgcagat gctgaagctg cctgacggca
    56941 ccgtcaaagt gctggtcgag gggttacagc gcgcgcgtat ttctgcgctc tctgacaatg
    57001 gcgaacactt ttctgcgaag gcggagtatc tggagtcgcc gaccattgat gagcgagaac
    57061 aggaagtgct ggtgcgtact gcaatcagcc agttcgaagg ctacatcaag ctgaacaaaa
    57121 aaatcccacc agaagtgctg acgtcgttga atagcatcga cgatccggcg cgtctggcgg
    57181 ataccattgc tgcacatatg ccgctgaaac tggctgacaa acagtccgtt ctggagatgt
    57241 ccgacgttaa cgaacgtctg gaatatctga tggcaatgat ggaatcggaa atcgatctgc
    57301 tgcaggttga gaaacgcatt cgcaaccgcg ttaaaaagca gatggagaaa tcccagcgtg
    57361 agtactatct gaacgagcaa atgaaagcta ttcagaaaga actcggtgaa atggacgacg
    57421 cgccggacga aaacgaagcc ctgaagcgca aaatcgacgc ggcgaagatg ccgaaagaag
    57481 caaaagagaa agcggaagca gagttgcaga agctgaaaat gatgtctccg atgtcggcag
    57541 aagcgaccgt agtgcgtggt tatatcgact ggatggtaca ggtgccgtgg aatgcgcgta
    57601 gcaaggtcaa aaaagacctg cgtcaggcgc aggaaatcct tgataccgac cattatggtc
    57661 tggagcgcgt gaaagatcga atccttgagt atcttgcggt tcaaagccgt gtcaacaaaa
    57721 tcaagggacc gatcctctgc ctggtagggc cgccgggggt aggtaaaacc tctcttggtc
    57781 agtccattgc caaagccacc gggcgtaaat atgtccgtat ggcgctgggc ggcgtgcgtg
    57841 atgaagcgga aatccgtggt caccgccgta cttacatcgg ttctatgccg ggtaaactga
    57901 tccagaaaat ggcgaaagtg ggcgtgaaaa acccgctgtt cctgctcgat gagatcgaca
    57961 aaatgtcttc tgacatgcgt ggcgatccgg cctctgcact gcttgaagtg ctggatccag
    58021 agcagaacgt agcgttcagc gaccactacc tggaagtgga ttacgatctc agcgacgtga
    58081 tgtttgtcgc gacgtcgaac tccatgaaca ttccggcacc gctgctggat cgtatggaag
    58141 tgattcgcct ctccggttat accgaagatg aaaaactgaa catcgccaaa cgtcacctgc
    58201 tgccgaagca gattgaacgt aatgcactga aaaaaggtga gctgaccgtc gacgatagcg
    58261 ccattatcgg cattattcgt tactacaccc gtgaggcggg cgtgcgtggt ctggagcgtg
    58321 aaatctccaa actgtgtcgc aaagcggtta agcagttact gctcgataag tcattaaaac
    58381 atatcgaaat taacggcgat aacctgcatg actatctcgg tgttcagcgt ttcgactatg
    58441 gtcgcgcgga taacgaaaac cgtgtcggtc aggtaaccgg tctggcgtgg acggaagtgg
    58501 gcggtgactt gctgaccatt gaaaccgcat gtgttccggg taaaggcaaa ctgacctata
    58561 ccggttcgct cggcgaagtg atgcaggagt ccattcaggc ggcgttaacg gtggttcgtg
    58621 cgcgtgcgga aaaactgggg atcaaccctg atttttacga aaaacgcgac atccacgtcc
    58681 acgtaccgga aggtgcgacg ccgaaagatg gtccgagtgc cggtattgct atgtgcaccg
    58741 cgctggtttc ttgcctgacc ggtaacccgg ttcgtgccga tgtggcaatg accggtgaga
    58801 tcactctgcg tggtcaggta ctgccgatcg gtggtttgaa agaaaaactc ctggcagcgc
    58861 atcgcggcgg gattaaaaca gtgttaattc cgttcgaaaa taaacgcgat ctggaagaga
    58921 ttcctgacaa cgtaattgcc gatctggaca ttcatcctgt gaagcgcatt gaggaagttc
    58981 tgactctggc gctgcaaaat gaaccgtctg gcatgcaggt tgtgactgca aaatagtgac
    59041 ctcgcgcaaa atgcactaat aaaaacaggg ctggcaggct aattcgggct tgccagcctt
    59101 tttttgtctc gctaagttag atggcggatc gggcttgccc ttattaaggg gtgttgtaag
    59161 gggatggctg gcctgatata actgctgcgc gttcgtacct tgaaggattc aagtgcgata
    59221 taaattataa agaggaagag aagagtgaat aaatctcaat tgatcgacaa gattgctgca
    59281 ggggctgata tctctaaagc tgcggctggc cgtgcgttag atgctattat tgcttccgta
    59341 actgaatctc tgaaagaagg ggatgatgta gcactggtag gttttggtac ttttgccgtt
    59401 aaagagcgtg ctgcccgtac tggccgcaac ccgcagaccg gtaaagagat caccatcgct
    59461 gctgctaaag taccgagctt ccgtgcaggt aaagcactga aagacgcggt aaactaagcg
    59521 ttgtccccag tggggatgtg acgaagttca agggcgcatc tactgatgtg ccttttttat
    59581 ttgtattcgg tgactttctg cgtcttgtgg gctgacaatt gcccccgttt cttgtcacaa
    59641 taggcctttg cgcgcatcga tacgttgcgt gaggtacaca gtcatctaca gcggagtgtt
    59701 gttacaccat gatggacagc ttacgcacgg ctgcaaacag tctcgtgctc aagattattt
    59761 tcggtatcat tatcgtgtcg ttcatattga ccggcgtgag tggttacctg attggcggag
    59821 gcaataacta cgccgcaaaa gtgaatgacc aggaaatcag ccgtgggcaa ttcgagaacg
    59881 ccttcaacag cgagcgtaat cgcatgcagc aacagctggg cgatcaatac tccgagctgg
    59941 cagcgaacga aggctatatg aaaaccctgc gtcaacaggt gctgaatcgt ctgatcgacg
    60001 aggcgctgct ggatcagtac gcacgtgagc tgaaactggg tatcagcgat gagcaggtta
    60061 aacaggcgat tttcgcgacc ccagccttcc aggttgacgg caaatttgat aacagccgct
    60121 ataacggtat cctcaaccag atggggatga ccgccgatca gtacgcccag gcgctgcgta
    60181 accagctcac tacccaacag ctgattaacg gcgttgccgg taccgatttt atgctgaaag
    60241 gtgaaaccga cgagctggcg gcactggtcg cgcaacaacg cgtggtgcgt gaggcgacta
    60301 tcgatgttaa cgcgctggcg gcgaagcagc ctgtgaccga acaggagatt gccagctact
    60361 acgaacaaaa caaaaacaat ttcatgacgc cggaacaatt ccgcgtgagt tacatcaagc
    60421 tggatgccgc aacgatgcag caaccggtta gcgatgcgga tatccagagc tactacgacc
    60481 agcatcagga tcaattcacc cagccgcagc gtacccgcta cagcatcatc cagaccaaaa
    60541 ctgaagatga agcgaaagcg gtacttgatg agctgaataa aggcggtgat tttgctgcat
    60601 tagccaaaga aaaatctgcc gatattatct ctgctcgtaa cggcggcgat atgggttggt
    60661 tagaagatgc cactatcccg gacgaactga aaaatgctgg tctgaaagaa aaaggccaac
    60721 tgtctggtgt catcaaatct tcggtcggtt tcctgattgt acgtctggac gacattcagc
    60781 cagcgaaagt gaaatcgtta gacgaagtac gtgacgacat tgcggcgaaa gtgaaacacg
    60841 aaaaagccct cgatgcgtac tacgcgctgc agcagaaagt gagcgatgcg gcaagcaacg
    60901 acaccgagtc tctggccggt gcagagcaag ctgccggcgt taaagccact cagacgggtt
    60961 ggttcagcaa agataacctg ccggaagagt tgaacttcaa gccggttgcc gacgctatct
    61021 ttaacggcgg tctggtaggt gaaaacggcg cgccgggcat caactctgac atcatcaccg
    61081 tagacggcga ccgcgcattc gtgctgcgca tcagcgagca caaaccggaa gcggtgaaac
    61141 cgttggcaga tgttcaggaa caagttaagg cactggttca gcacaacaaa gctgaacaac
    61201 aggcgaaagt ggatgctgag aaactgctgg ttgatttgaa agccggcaaa ggtgcggaag
    61261 ctatgcaggc tgccggtctg aaatttggcg agccgaaaac cttaagccgt tccggtcgtg
    61321 acccgattag ccaggcggcg tttgcactgc cactgccagc gaaagacaaa ccgagctacg
    61381 gtatggcgac cgatatgcaa ggtaatgtgg ttctgctggc gctggatgaa gtgaaacaag
    61441 gttcaatgcc ggaagatcag aaaaaagcga tggtgcaggg tatcacccag aacaacgcac
    61501 aaatcgtctt tgaagctctg atgagtaacc tgcgtaaaga ggcgaaaatc aaaattggcg
    61561 atgcgctgga acagcaataa tcctgaagcc gcctcgcaaa aaaatgcgtt gcagctgtaa
    61621 caagaaaagg tcgctttcgc ggccttttcc atttctgaac attgccattt gtttactgtt
    61681 ttcactgccg ttaaggtgat tccactgtta acaaacaagg agaaaacagt atgaaacacg
    61741 gaattaaagc actgctcatt accctgtccc tggcctgtgc cggaatgtct catagcgcgc
    61801 tggcggcagc ttctgtggcg aaaccgacaa cggtagaaac caaagcggaa gctcctgcag
    61861 cacaaagtaa agcagcagta ccggcgaaag ccagtgacga agaaggcacc cgggtcagca
    61921 ttaataatgc cagcgcggaa gagctagccc gcgcgatgaa tggcgttggc ctgaagaaag
    61981 cgcaggcgat tgtcagttat cgcgaagagt acggtccgtt taaaacggtg gaggatctaa
    62041 agcaggtgcc ggggatgggc aattcgctgg tggaacgtaa tctggcggta ttaaccctgt
    62101 aattaatttg catagtggca attttttgcc agactgaaga ggtcatacca gttatgacct
    62161 ctgtacttat aacaacaacg taaggttatt gcgctatgca aacacaaatc aaagttcgtg
    62221 gatatcatct cgacgtttac cagcacgtca acaacgcccg ctaccttgaa tttctcgaag
    62281 aagcccgctg ggatgggttg gaaaatagcg acagttttca gtggatgacg gcccataaca
    62341 tcgccttcgt cgtggtcaat atcaatatta actatcgtcg cccagcggta ttaagtgacc
    62401 tgttaactat taccagtcag ttgcagcaat taaacggtaa aagcggcatc ttaagccagg
    62461 tcattacact ggagccggaa gggcaggtgg tagcggatgc gcttattacg tttgtttgta
    62521 ttgatcttaa aacgcagaaa gcattagctc tggaagggga attgcgcgaa aagctggagc
    62581 agatggttaa gtaaacgttt tgtggtgccg gatgctcaag ccgcatccgg cgacacccgg
    62641 aataattacc tcaacccggt tttctgcttc atcgctgcca tcaccgtcgg tttatcggcc
    62701 agataatgat tcaaaccgtt ggcgcgtaaa ttacatgccg cacaatgacc gcaaccgtcg
    62761 cctttaatgc cgttatagca ggtcaacgtt tcgttacgga ctaaatccag tttgccgtaa
    62821 taatctgcca gcgcccaggt ttccgcttta tcaatccaca tcagcggcgt ttcaaaacga
    62881 atatctttcg ccatgcccaa actgacggca tggtttagtg ctttcacaaa ctcatcgcgg
    62941 caatccgggt agccggagaa atccgtttca cagacgccgg taattacggc ttctgctttt
    63001 acctgatacg catatattgc cgccagcgtc aggaacaaaa tattacgccc tgggacaaac
    63061 gtattcggga tgccatcggc ttcaggttca taatcaggca ccggaatgct gtcacgtgtc
    63121 aggctgctga ccgccagctc gttgagcagc gttacatcaa gaaccttatg cgcgcgtgct
    63181 cccagtttca gcgccagttc gcgtgccacg tcgatttctg cgcgatgtcg ctgaccgtaa
    63241 tcgaacgtca cgcaatggac ttcatcatat tgttgtaatg cctgcaccag acaggtggtg
    63301 gaatcctggc ctccactgaa cacaacgaca gcacgtttca tagataatcc tgcttgaaca
    63361 ataaaagcgt tatggtaacg cctgcgatta acccggacca gctattcatt gcgacggcgc
    63421 gggaagccag gcttcggtaa attcaaacca gccgcgcggt gtcagtcgca caccgttcac
    63481 gccgggaggg gcactaatgc gatagtgata gttgaacagc ggcgtcagcg tcgcgtctgc
    63541 cattaactgg ctaaaaaccg ctttcagggc attaaatcgg ttttcctcat caggcattac
    63601 ttgcaccgcg tccagcgtcg attgcagatg tgcatatgct ggagcgtcga aaacatgtgg
    63661 ccatagtgga tcgcaacgta gccattgctc cagagtatat tccggtgctt cgccaattaa
    63721 tctgtcgccc atcatgaggt ctgcgtgtgc cagtagggtc gtgtcgtccc agttttttgc
    63781 gttatgaaaa ataattgtga gttcacagcc ttccgctgcc agtgtcgcct gtaggcgttc
    63841 tgccatggta tgaagttcta tcggtaagtg ataaaccagc gtcaaggttt tcggtagttt
    63901 gacttcatcc ggtacttgcc agtgcggaat agtccagcct ggcagtaatg catgactggc
    63961 ggtgatcagg ttttctccga cttctaacgt ttgtaataaa ccggattgat gaataatgga
    64021 gatcactttt cgcgcctgcc agagggagag tcggggactt ttgcgcaacg ttaaatagca
    64081 aaaacctaaa ctgatgccac tactgacctg gctgacccgt tgcagctcct ccggtttgcc
    64141 gatggtgatt tgcacgggat gccgacaact agttcccaaa tctttttcga aaagcggcgg
    64201 agttatccag tactcaaccg ctttaagcag cggatgacgc aaatggtaat aatcatggct
    64261 ctccaggcgc accagctccg ctgtgaattg tgttaagcga aaaggaccag tgccgatcag
    64321 tgggaattgc ggatgcgcca gatggctgca atagctcgcc agccggtgcg caagccagta
    64381 atcggggcga tgtaaaaaga aggtcagaca ctgcggatgg gtgacctcaa tacgcttcac
    64441 gctaataaat aattgatcca gtgctggcag ttgtaacagc atcaataatc gctggtgtaa
    64501 gtgtgaggct tttactgcat cgccgttatg ccagtgtagg gttgaacgaa gataaaagtc
    64561 ccagcgtaac ccgtcagtag aggtttccca gtgatgcgct aaatcgccaa tcgggcgctg
    64621 agtattatta tcgaagcggg tcaggccgga aaatatctgc ccggcgagat gctgctcggc
    64681 acggccgggc aaaaagcctg gttgtagcgg ttcgagcggg cgatagtagg gaatacgcaa
    64741 cgtgggtgta tcgttttgcc attgtccgcc cataaacggc tgtaacagag tgcgcagctc
    64801 acctggggcc agttgcgcca gctccagcac atcttgctgc tttccggttt ccagtgcctg
    64861 ttccatcatc gcattgcgta gcgattccgg cgtgaccaga aagcgtaatt gtccgcgctt
    64921 tccgcgtcct gactgcgcct gccactccag ccatcccgcc tcctgtgcct gacgcaacag
    64981 cgtacgaaca tggcgttcgc tgcaaaaaca gcgttcggcc agttcgctga cggtgacggt
    65041 ttgcggcttt ccggcggaag gttgccacag acgttgatac tggttaagac ggttgagcaa
    65101 tcgcatataa acccggaaca atattattta actattcact attacttccg tatatatcag
    65161 gtgatactca atcaccatta accgtgtcac agagtggaga aagaaatggc tcgtctggca
    65221 gcatttgata tggatggcac tttattgatg cctgaccatc atttaggtga gaaaaccctc
    65281 tctactttgg cgcgactgcg tgaacgcgac attaccctca cttttgccac ggggcgtcat
    65341 gcgctggaga tgcagcatat tctcggggcg ctatcgctgg atgcgtattt gattaccggc
    65401 aacggaacgc gcgtgcattc tctggaaggt gaacttttac atcgtgatga tttacctgcg
    65461 gatgtcgcgg agctggtgct gtatcagcaa tgggataccc gagccagcat gcatatcttc
    65521 aatgacgacg gttggtttac cgggaaagag atccctgcgt tgttgcaggc atttgtctat
    65581 agcggttttc gttatcagat aatcgatgtc aaaaaaatgc cactcggcag cgtcaccaag
    65641 atctgcttct gtggcgatca cgacgatctt acacgcttgc agatccagct atacgaagca
    65701 ttaggcgagc gtgcacattt gtgtttttcc gccacggatt gcctcgaagt gctgccggtg
    65761 ggctgcaata aaggcgctgc attgacggtg ctgacccaac atttaggttt atcgttgcgc
    65821 gattgcatgg cctttggtga tgcgatgaac gatcgcgaaa tgttaggcag cgtcggtagc
    65881 ggatttatta tgggcaatgc gatgccgcaa ctgcgcgcgg agctcccgca tttaccggtg
    65941 attggacatt gccgaaatca ggctgtctct cactatttga cgcactggct ggactatcca
    66001 catctacctt attcccccga ataacgagat cccttccagc accgggcaat tgcccggttt
    66061 tttttgcgtt gaatttgtca ttttgtgccg tggtgtttaa accgcacaga ataaattgtc
    66121 gtgatttcac ctttaaaata aaattaaaag agaaaaaaat tctctgtgga agggctatgt
    66181 tagataaaat tgaccgtaag ctgctggcct tactgcagca ggattgcacc ctctctttgc
    66241 aggcactggc tgaagccgtt aatctgacaa ccaccccttg ctggaagcgc ctgaaacggc
    66301 tggaggacga cggtatcctt atcggcaaag tcgccctgct ggatccggaa aaaataggcc
    66361 tcggcctgac cgcttttgtg ctgataaaaa cgcaacatca cagcagcgaa tggtattgcc
    66421 gctttgtcac ggtggttacc gaaatgccag aagtgctggg gttctggcgc atggctggtg
    66481 aatacgatta tctgatgcgc gtccaggttg ccgacatgaa acgctacgac gagttttata
    66541 agcgtctggt aaacagcgtg ccggggctgt cggacgtcac ttccagcttc gcgatggaac
    66601 agattaaata caccacttct ttacccatcg aataaatatc cagaatcagg tcaggacaca
    66661 acgcgtgcga ttatttgctc aattaagctg gtatttccgt cgggaatggc gtcgctatct
    66721 cggggctgtc gccttgcttg tcattatcgc gatgctgcaa ctggttccgc caaaagtggt
    66781 tggtattgtt gtcgatggcg tgacagaaca acactttact accgggcaga tcctgatgtg
    66841 gatcgccacc atggtgctga ttgccgttgt ggtttatctc ctgcgttacg tctggcgggt
    66901 attgctgttt ggtgcgtctt atcaactggc tgttgaactg cgtgaagatt attaccgtca
    66961 gctaagccgg cagcatcctg agttttacct gcgtcatcgc accggtgacc tcatggctcg
    67021 tgcgaccaat gacgtcgatc gcgtcgtgtt tgccgccgga gaaggggtgc tgacgctggt
    67081 ggattcactg gtgatgggct gcgctgtgtt gattatgatg tctacgcaaa ttagctggca
    67141 gttgacctta tttgccctgt tgccgatgcc agtgatggcg atcatgatta agcgcaacgg
    67201 cgatgctttg catgaacgct ttaagctggc acaggcggcg ttttccagtc ttaatgaccg
    67261 cacccaggaa agcctcacca gtatccgcat gatcaaagcc tttggtctgg aagatcgcca
    67321 gtcggcgtta tttgccgcgg atgccgaaga taccggcaaa aaaaacatgc gggtggcgcg
    67381 tattgatgct cgtttcgacc cgaccatcta tatcgcgatt ggtatggcga acttgctggc
    67441 gattggcggc ggtagttgga tggtggtgca gggcagttta acgctgggcc agctcaccag
    67501 ttttatgatg tatttaggtc tgatgatttg gccaatgctg gcgctggcat ggatgtttaa
    67561 cattgtggaa cgtggtagtg ctgcgtacag ccgtattcgc gcgatgctgg cggaagcgcc
    67621 ggtggtgaac gatggtagcg aacccgtgcc ggaagggcgt ggcgaactgg atgtaaatat
    67681 tcaccagttc acgtatccgc agactgacca tcctgcgctg gaaaacgtca atttcgccct
    67741 gaaacccggt cagatgctgg gtatctgcgg gccgactggt tccggcaaaa gtaccctgtt
    67801 gtcgctcatt cagcgtcatt tcgacgtcag cgagggggat attcgctttc atgatattcc
    67861 tctgacgaag ttacaactcg atagctggcg tagccgcctg gcggtagtta gccagacgcc
    67921 attccttttt tctgacactg tggcgaataa catcgcgctg ggttgcccga atgccaccca
    67981 gcaagagatt gagcatgtcg cgcggttagc cagcgtacat gacgatattt tgcgtctacc
    68041 gcaaggttac gatacagagg tgggcgagcg cggtgtgatg ctttccggcg ggcaaaaaca
    68101 gcgtatctcc attgctcgtg cgttattagt caacgcggaa atcctcatcc ttgatgatgc
    68161 gctttcggcg gtggacggac gcactgagca ccagatcctg cataacctgc gtcagtgggg
    68221 gcagggaaga acggtaatca tcagtgccca tcgcctttct gcactgacgg aagccagtga
    68281 aattattgtg atgcagcacg gacatatcgc ccagcgtggc aatcatgatg tgctggcaca
    68341 acaaagcggc tggtatcgcg atatgtatcg ctatcaacaa ctggaggcgg cgctcgacga
    68401 cgctccggaa aatcgcgagg aggccgtcga tgcgtagttt tagccaactg tggccgactc
    68461 tcaagcgcct gttagcgtac ggttcgccgt ggcgtaaacc gctggggatt gcggtcctga
    68521 tgatgtgggt tgcggcagcg gcagaagtca gcgggccgct gcttatcagc tattttatcg
    68581 acaatatggt agcgaaaaat aacctgccgt tgaaagtggt tgcagggctg gctgcggcgt
    68641 atgttgggct acaactgttt gccgccgggc tacattacgc gcagtcgctg ctgtttaatc
    68701 gggcggcagt aggcgtagtg caacagttgc gtaccgacgt gatggatgct gcgttacgcc
    68761 agccattaag cgagtttgat acccaacccg tcgggcaggt gatttcccgc gtcactaatg
    68821 acactgaagt gatccgcgat ctctacgtta ccgtagtggc aactgtcctg cgtagcgccg
    68881 cactggtggg cgcgatgctg gtggcgatgt tcagcctcga ctggcgaatg gcgctggtgg
    68941 cgataatgat tttcccggtg gtgctggtgg taatggtgat ataccagcgt tacagcacgc
    69001 cgattgtccg tcgtgtgcgc gcctatctgg cggatatcaa cgacggcttt aacgaaatca
    69061 tcaatggcat gagcgtcatc cagcagttcc gtcagcaggc gcgatttggc gaacgtatgg
    69121 gtgaggccag tcgttcacac tatatggcga ggatgcaaac cctgcgcctc gacggttttc
    69181 tgctgcgtcc gctgctgagt ctgttttcat cgctcattct ttgtggattg ttgatgctgt
    69241 ttggcttctc cgccagcggc accattgaag tgggcgtgct gtatgccttt atcagctatc
    69301 ttgggcgact taacgaaccc ttaatcgaac tgaccacgca acaggcgatg ctgcaacagg
    69361 ctgttgttgc tggtgagcgc gtgtttgaac tgatggacgg accgcgccag caatatggca
    69421 atgatgatta cccgttacag agtggtacca tcgaagtcga taacgtgtca tttgcttatc
    69481 gcgatgacaa tctggtgcta aagaacatta atctctctgt gccttcgcgt aactttgtgg
    69541 cgctggtcgg gcataccggc agtggtaaaa gtaccctcgc cagtttattg atgggctatt
    69601 acccgctaac ggaaggtgag attcgccttg atggtcgtcc attaagttcg ctaagtcaca
    69661 gcgcgctgcg ccagggcgtg gcaatggtgc agcaagatcc ggtggtgctg gcggatacct
    69721 tcctcgccaa cgtgacactg gggcgggata tctccgaaga acgcgtctgg caggcgctgg
    69781 aaaccgtgca actggcggag ctggcgcgta gcatgagcga cggtatttac acgccgctgg
    69841 gcgagcaggg gaataatctc tcagttgggc aaaagcaact gctggcactg gcgcgcgtgc
    69901 tggtggagac gccgcaaatc ctgatccttg atgaggcaac cgccagcatt gactccggta
    69961 ctgaacaggc gattcaacat gctctggcgg ctgtgcgtga acataccacg ctggtggtga
    70021 ttgctcaccg cttatcgacc attgttgatg ccgacaccat tctggtgctt catcgtgggc
    70081 aagccgtgga gcagggcact caccagcaac tgctggcggc ccagggacgc tactggcaga
    70141 tgtatcaact gcaacttgcg ggcgaagagc tggcagccag cgtgcgtgaa gaagaatcat
    70201 tgagcgcctg aatagcgcaa tatttcatcg ttggtgcaaa aatgtaacgc actgtgcact
    70261 gtcatagtgc gttttcattt tcaatctttt taacttcctg ctctctttct cgtttttcat
    70321 ttctggcaca ccgcttgcaa taccttcttc gtgtagcaga accattaccg aattctgacc
    70381 ggaggggatc tatgaagctg gtgaccgtga taatcaaacc attcaagctg gaagacgttc
    70441 gtgaagcgtt atcttccatt ggtattcagg gcctgaccgt caccgaagtg aaaggtttcg
    70501 ggcgtcagaa agggcatgcc gagctgtacc ggggggcgga atacagcgtc aacttcctgc
    70561 caaaagtaaa aattgatgtg gcgattgctg atgaccaact cgatgaagtg atcgatatcg
    70621 tcagtaaggc ggcttacacc ggaaaaattg gcgacggcaa aatcttcgtc gctgaattgc
    70681 aacgcgtcat tcgtattcgt accggcgaag ccgacgaagc ggcgctgtaa tctctggcac
    70741 acagcaacag gaacgaaaaa tgaagatagc gacgataaaa actgggcttg cttcactggc
    70801 gatgctgccg ggactggtaa tggctgcacc tgcggtggcc gataaagccg acaatgcgtt
    70861 tatgatgatt tgtactgcgc tggtgctgtt tatgactatt ccggggattg ccctgtttta
    70921 cggtgggttg attcgcggca aaaacgtgct gtcgatgctg acgcaggtga cggtgacatt
    70981 tgcactggtc tgtattctct gggtggttta cggttactcg ctggcgtttg gtgagggcaa
    71041 caacttcttc ggcaacatta actggctgat gctgaaaaac atcgaactga cggcggtgat
    71101 gggcagcatt tatcagtata tccacgtagc gtttcaggga tcgtttgcct gcattaccgt
    71161 aggcttgata gttggggcgc tggcggaacg aatccgcttc tcagctgtgt tgattttcgt
    71221 agtggtatgg ctgacgctct cttacattcc gattgcgcat atggtgtggg gcggtggttt
    71281 gctggcttct cacggtgcgc tggatttcgc gggtggcacc gtggtgcaca ttaacgccgc
    71341 aatcgccggt ctggtgggcg cgtatctgat aggcaaacgc gtgggcttcg gcaaagaggc
    71401 gtttaaaccg cacaacctgc cgatggtctt caccgggact gccattctct atatcggttg
    71461 gtttggcttt aacgccgggt cagcgggcac ggcgaatgaa atcgcggcac tggcatttgt
    71521 gaatactgtg gtcgcaacgg cggcggcaat tcttggctgg atcttcggtg aatgggcgct
    71581 gcgtggtaag ccttcactgc tgggggcgtg ttctggcgcg attgccggtc tggtcggcgt
    71641 gacgccagcg tgtggctaca ttggggttgg cggcgcgttg attatcggcg tggtagctgg
    71701 tctggcgggc ttgtggggcg ttaccatgct caaacgcttg ctgcgggtgg atgatccctg
    71761 cgatgtcttc ggtgtgcacg gcgtttgcgg tattgtcggc tgtatcatga ccgggatttt
    71821 tgccgccagt tcgctgggtg gcgtgggctt cgctgaaggt gtgacgatgg gtcatcagtt
    71881 gctggtacag ctggaaagca tcgccattac gatcgtctgg tccggtgtgg tggcatttat
    71941 tggctacaaa ctggcggatc tgacggttgg tctgcgtgta ccggaagaac aggagcgaga
    72001 agggctggac gtcaacagcc acggcgagaa tgcctataac gcgtaacaag cactgcaaaa
    72061 aacagccgga cggttttcac ctccggctat ttttttaatt gtgattacgc atcacccctt
    72121 cctgaacggt cgaggcaacc agtacgccgt cctgggtata aaactcaccg cgcacaaagc
    72181 cacgtgcgct ggacgccgag gtgctctcca cgctatacag cagccattca ttcaaattaa
    72241 acgggcgatg gaaccacatg gaatggtcaa tggtggcaat ctgaatcccc ggttcgagaa
    72301 aaccgatgcc gtgcggctgt agagctaccg gcaggaagtt aagatcagaa gcgtaaccga
    72361 gcagatactg atgaacgcgc aggtcatccg gcacgctacc atttgcgcgg atccacacct
    72421 gacgatgcgg ttctgcgacg tgacctttca gtgggttatg aaactctacc ggacggactt
    72481 ccagcggacg atcgcagatg aatttatctt tcagcactgg cggcagcagg tgcgccagcg
    72541 actgggcgat ttgcgtttcc gaaggcaggc catcaggcgc tggcgcggac ggcattgttt
    72601 tttgatgttc gaaacccgct tctggtgcct ggaaagaggc cgtcatataa aaaatcggtt
    72661 tgccgttttg aatagcagca acccggcggg cgctgaagct gttaccgtca cgcagcgttt
    72721 cgacatcata aataatcggc ttcttactat cgccagggcg aagaaagtag ctgtgaaacg
    72781 aatgtaccag ccgctcttca gggacggtct cttttgcagc atacaaggcc tgacccacga
    72841 cctggccgcc aaacacctgg cgtaaaccta aatcttcact ctggccgcga aagagtcctt
    72901 cctcaatttt ttccagattt aacaatgtca gtaaattttt tagcgcctga ctcatataac
    72961 tctccagtaa caaagctgcc gcagcaagcc aaagtgagtt gagtataacg caaatttgtc
    73021 actggtccga tgggtgcaat ggtctgaatt acgggctaat tacaggcaga aatgcgtgat
    73081 gtgtgccaca cttgttgacg ttactatttt gttaaccact cttccggcga ggaaagttag
    73141 cccgctggtg cattgataat aaggagaaat gaatgaaact cgtgcacatg gccagtggtt
    73201 tagcggttgc gattgcgttg gcggcttgcg cagataaaag cgcggatatt cagacgccag
    73261 caccggctgc aaatacgtct atttcagcaa cacaacaacc agctatccag caaccgaatg
    73321 tctccggtac cgtctggatc cgtcagaaag tcgcactgcc gcctgatgct gtgctgaccg
    73381 tgacactttc tgacgcgtcg ttagccgatg caccgtcaaa agtgctggcg cagaaagcgg
    73441 tgcgtactga aggtaaacag tcaccattca gctttgttct gccatttaac ccggcagatg
    73501 ttcagccgaa cgcgcgtatt ctgttgagtg cggcgattac cgtgaatgac aaactggtat
    73561 ttatcaccga taccgttcag ccggtgatca accagggcgg aactaaagcc gacctgacat
    73621 tggtgccggt acagcaaacc gccgtgccgg ttcaggccag cggtggcgca acgactaccg
    73681 taccttcgac ttcaccaact caggtgaatc cgtcttcggc agttccagct cctacgcaat
    73741 attaagcgac gttaaccctc tccgcccgga gagggttagt agttccagcg ataacgctgc
    73801 aagtcgattt gcccgcttcc cgataccatc acaccttctg ccagtaatgc ctgtcgctga
    73861 cgctgtaaat ccggtccggt tagcgaaatt gtgccgtggc gattaaccac ccggtgccag
    73921 ggtaaggtgc tgccttcagg gagacgcttt aacacaccgc ccacctggcg cgcggcgcgg
    73981 ggcgatcccg ccagtttcgc cacatcaccg taagtggtga catagccttc gggaatagcg
    74041 gcgacgattt gccagacgcg ttggggaaat gaatcttctt tttccatctt ttcttcctga
    74101 ggtaattttt cagcataatc tggaaaaacg cccgagtgaa gtcgcattgc gcaagaaacc
    74161 agcatctggc acgcgatggg ttgcaattag ccggggcagc agtgataatg cgcctgcgcg
    74221 ttggttctca acgctctcaa tgggggctct gttggttctc ccgcaacgct actctgttta
    74281 ccaggtcagg tccggaagga agcagccaag gcagatgacg cgtgtgccgg gatgtagctg
    74341 gcagggcccc cacccatttc tgcctcccac cgtttcgtca aaaaatctca atatggctaa
    74401 actttaacca caactgacgt cgcaagaatt gtctggctgc gcagtacgct tcggaggtat
    74461 gtctgatgaa gtatgttgat ggttttgtgg ttgccgttcc tgccgataaa aaggatgcct
    74521 atcgggaaat ggccgctaag gctgcgccat tgtttaaaga gtttggcgcg cttcgtattg
    74581 tcgaatgctg ggccagcgat gtaccggatg gcaaagtgac cgattttcgt atggcggtga
    74641 aagcggaaga gaatgaagag gttgtcttta gctggattga atacccttca aaagaggtcc
    74701 gcgacgctgc taatcaaaag atgatgtcgg acccacggat gaaagagttc ggcgagtcca
    74761 tgccgtttga cggcaagcga atgatctatg gcggattcga gtcaatcatc gacgaatagg
    74821 tagcgtgacg ggctgtgcgg cgtgccgtca gcccaaaaat tcacaaatgt tgctcggccc
    74881 agcgtaagaa atcttctttc ggtaatgcct tgctgtagag ccagccctga ccgtagtgca
    74941 cgccatgctg gcgtaaccac tcttcttgtt tactggtttc gattccctcc gctaccattt
    75001 tcagtttcag tgtttttgcc atttcgatga tatgcggcgt gacattttta tattccagcg
    75061 catcaacgaa agatttatcg atcttcagaa tgtcgacatc cagatcctgt aaataactta
    75121 aacttgaata ccccgtacca aaatcatcaa gataaatttc atggcccgcc tcccggtagc
    75181 gagaaattat cggggcgctg gttttcggat cggcaaactc gcgttcagtg agttcaagcg
    75241 cgatctgtct gggattaacc tgatagtgat tgatcatgtc acgcagcaat tgcgggattt
    75301 tttccgaggt gagcacggtg gattcaagat tgatcgaaat atgctgctgt ggatgctgac
    75361 gcagccagtc gcccatatct tcaaagacgc ttcttataat cagtagcgtc aatggctcag
    75421 aaaggcccgt ttgctgtgcc agcggaataa aactatctgg tgacaaccaa ctaccgtctg
    75481 tctgcggcca gcgcgccagt gcctcagcac cgacaatttt gccattggct aaggagacaa
    75541 tcggctgata gtgcacgcaa atatcacgat tttcgatagc atcctgcagc cgatgatgcg
    75601 gtgactgaat acggcgcaaa atacgcagca caaacatcgc tgccagcagg ccaatcacca
    75661 acccgagcgg taaccaaata aagacttgcc gatgccagcc tttctgcaac attttcgttg
    75721 aagcccatgt gatgatcgaa atattcatct ccggtaaggg caggatatcg taaatgattc
    75781 cattattttc gatatgctca ccgggtgttt tttgtagcct ggtaataatt ccctgagcaa
    75841 tttcatcgct gctggttatg acaacgttat gggcattgcc aataatggcg gcatcaattt
    75901 gccatgagct atagggaatg acatcaataa aggaagcggg gtcgatcatg acgacataat
    75961 gtgccgttcc catggcgacc atgtaacgga taatgcctaa atcgttatgc gatgttaacc
    76021 agacacgata accatctttc gaaattttac ctggctcggg gaaggtatcg ggcggacttt
    76081 catgctccag agacgaacac tggggaacgt tgttatcgat ataagccact tcctgaatat
    76141 agcgataact gtaagatacc cgacgcattt ccatgagatg ggcttcgcta caggcagcgc
    76201 cttgccatct ttccagctcc tgcagcgcat ctttcccttg tgtcgccacc ttattggctc
    76261 gaatagcgac gcgggaggaa taggtatcca gctcttcaat aaacgatgtt tctacctgct
    76321 gatgggccag ccagatgctt aagccgacag gcagcaatac tgaaagaatc agtactcccg
    76381 aaataaggcc gaccagatgt cgtgttctca ccagcatatc cttattaaca cagtgtaggg
    76441 aaagtatatc tgattgtgga aaaaggctgg ctggagataa ggaggtagag ctggtatttc
    76501 atgaagtgat gggtggaaac gatcattgtc atttgaccag gcgcagagct ttacgcctgg
    76561 tatgtaaccc taacttaaga tgttgattcg gcgcgagcga gggtaaaaat atcgtaaaaa
    76621 gacagttcac ctttacggac gatcattttt tgcagttgtt ccttatgaac atccgcgaca
    76681 cgcggagagt tcaagatggc ttcaaccagc gcggcaggta caaagcgcgt gttgtaccat
    76741 tcgccgttat agcaaacgcg gaaatccagc acgtcgatat cttcatagcg gtagcgtggg
    76801 tagacatcga aaaagaagaa accattaagc aggataaata atacaaccag tagccagaca
    76861 gagcctgaca gcgtttcgga ctgcaacatc accgccaggg tggcaaaaaa agcaacgtac
    76921 ataccgataa acagccccgg atgtttacgg ataaaactga tactaaagcg gggtttgttg
    76981 tcgcgttttt cgcgggtatt cagagactca atcgtttcgg tcagcaggcg ttgtatttcg
    77041 gtcatcgttt gccttcgtgg tatctgcaac tttccaggga acaccctatt ttaacgtggg
    77101 aatagtagag gggaagtaac agatctacca caattttgca taacagttgc gaaaaaccga
    77161 ttacaatttt ttaattattc tggctggatt accgcccacg acaacgttgt ccgggacatc
    77221 ttttgtgaca actgcacctg aggctaccac gacgttatca ccaatggtca caccagggtt
    77281 aatgaccgcg cgtccgccaa tccagacgtt attaccgatg gtgacgggtt tccccagttc
    77341 agcaccgcta ttacgtgcta cagggtcgat gggatgtgtt gccgtgtaga tatgaacgcc
    77401 tggtgccaac atacagttat caccgatgcg aatagggcag acatcaagca tcacgcaatc
    77461 gaagttggcg aaaaaattat taccgagaaa aatgttatag ccatagtcac agcgaaacgt
    77521 tggctcaata taagcctctg tcacctgacc gaatagatca gcgagaattt gctggcgtaa
    77581 tgtgtgctct tccgccaggg aatgattgta tcggtgaata agctgacgag cgcgcaggcg
    77641 atcgcgagat aacgtctcat ctgccgagcg atacaactca ccagcaatca tcttttcttt
    77701 ttctgtgctc attactgacc tccggttgct gaaaatgcgg caacacagta agacgaaaag
    77761 tcagtgaaga gaacgttccc attacatctg tgtgataaag atcacatagg gtatgtcttc
    77821 atggtgaaaa gtataaaatt cttaataaac agccggttat agctccgaaa gcgaattatt
    77881 tattagcgaa taaatttcca tactgaggaa gggatcttgt cgtatagttt attcatggtc
    77941 aattcggcga ggcggtgatc tgcggctgag taaaataccg ccagttcatt atctgataat
    78001 tcgtatttat ttttctcgat aacacgctcc agcgtgtcaa ttgtctggca acgacgtaaa
    78061 cgcattaaat aatcggtttt cgttaaaggt ttttcggaca tacttctacc catggttgta
    78121 ataattctaa caagataaac tcgcaggatt ctctttcgtc gcattgacaa aacaacggaa
    78181 tagtcgatta cctgacttcc gccatttctg aagatcctgc atattaatac cataactact
    78241 gaacaacata aaggtgtcat ccagatattc gtcgatctgc tcaatgagct tattgtcttc
    78301 attatactta attttgtaat taagtgcgaa ggtcgcaata tgctcaatca gttcattcaa
    78361 ctggaggttg atcgccgagg ttgggtcgtt aacccagcca tgattgcttt cttcaaggtt
    78421 tgcaaggcag tcatgataca gggtttcaca gagaaactta agctgtgcga tatcatgtct
    78481 tttgggtgag tattcatcca taacgcgtcc ccttcttagc ggttgaacta acggacacct
    78541 ttcgggatgg aaaaaactta ctgacctgga cttgccctct cgtttgttag cttaactata
    78601 agccactctt tgcaggtttt catcgcatta ttacgaaaaa ttacaattag gaaggaagcg
    78661 ggacaggact cactttttca tactagcacc aacccgcagt aggtaaaagc agtttgctcg
    78721 aacagtttat aacatttcag gattatcgcc agatgacatt ggtgacacta ataccaggat
    78781 tgctctgaat atgacgtaat aaccgaggaa tgaataaaga a
//
LOCUS       DAJOSY010000007        77795 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJOSY010000007 DAJOSY010000000
VERSION     DAJOSY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.007"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..811)
                     /gene="mppA"
                     /locus_tag="OHN27_000515"
     CDS             complement(<1..811)
                     /gene="mppA"
                     /locus_tag="OHN27_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCQ2285020.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPK"
     gene            complement(1148..2047)
                     /gene="pgrR"
                     /locus_tag="OHN27_000516"
     CDS             complement(1148..2047)
                     /gene="pgrR"
                     /locus_tag="OHN27_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCQ2285021.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2185..3105
                     /locus_tag="OHN27_000517"
     CDS             2185..3105
                     /locus_tag="OHN27_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2285022.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            3176..3313
                     /locus_tag="OHN27_000518"
                     /pseudo
     CDS             3176..3313
                     /locus_tag="OHN27_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
     gene            3440..4168
                     /gene="mpaA"
                     /locus_tag="OHN27_000519"
     CDS             3440..4168
                     /gene="mpaA"
                     /locus_tag="OHN27_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCQ2285023.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4143..5108)
                     /gene="ycjG"
                     /locus_tag="OHN27_000520"
     CDS             complement(4143..5108)
                     /gene="ycjG"
                     /locus_tag="OHN27_000520"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCQ2285024.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARR
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5227..5733
                     /gene="tpx"
                     /locus_tag="OHN27_000521"
     CDS             5227..5733
                     /gene="tpx"
                     /locus_tag="OHN27_000521"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCQ2285025.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(5777..7318)
                     /gene="tyrR"
                     /locus_tag="OHN27_000522"
     CDS             complement(5777..7318)
                     /gene="tyrR"
                     /locus_tag="OHN27_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCQ2285026.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(7466..8527)
                     /locus_tag="OHN27_000523"
     CDS             complement(7466..8527)
                     /locus_tag="OHN27_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCQ2285027.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(8524..9921)
                     /locus_tag="OHN27_000524"
     CDS             complement(8524..9921)
                     /locus_tag="OHN27_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCQ2285028.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMSEGLDPLAPADENRLADIAAAWTDYLHHCKEQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            10077..11075
                     /locus_tag="OHN27_000525"
     CDS             10077..11075
                     /locus_tag="OHN27_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2285029.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALKAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(11186..12091)
                     /gene="ompG"
                     /locus_tag="OHN27_000526"
     CDS             complement(11186..12091)
                     /gene="ompG"
                     /locus_tag="OHN27_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCQ2285030.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(12136..13218)
                     /locus_tag="OHN27_000527"
     CDS             complement(12136..13218)
                     /locus_tag="OHN27_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2285031.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVSGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(13232..13891)
                     /gene="pgmB"
                     /locus_tag="OHN27_000528"
     CDS             complement(13232..13891)
                     /gene="pgmB"
                     /locus_tag="OHN27_000528"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCQ2285032.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            complement(13888..16155)
                     /gene="ycjT"
                     /locus_tag="OHN27_000529"
     CDS             complement(13888..16155)
                     /gene="ycjT"
                     /locus_tag="OHN27_000529"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCQ2285033.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGSYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAYTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLITVAEESVFCLGDF
                     ILPFNGTATKHQEDE"
     gene            complement(16152..17207)
                     /locus_tag="OHN27_000530"
     CDS             complement(16152..17207)
                     /locus_tag="OHN27_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCQ2285034.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(17217..18004)
                     /locus_tag="OHN27_000531"
                     /pseudo
     CDS             complement(17217..18004)
                     /locus_tag="OHN27_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(18023..19075)
                     /locus_tag="OHN27_000532"
     CDS             complement(18023..19075)
                     /locus_tag="OHN27_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCQ2285035.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19106..19948)
                     /locus_tag="OHN27_000533"
     CDS             complement(19106..19948)
                     /locus_tag="OHN27_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCQ2285036.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(19935..20816)
                     /locus_tag="OHN27_000534"
     CDS             complement(19935..20816)
                     /locus_tag="OHN27_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCQ2285037.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(20837..22129)
                     /locus_tag="OHN27_000535"
     CDS             complement(20837..22129)
                     /locus_tag="OHN27_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2285038.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWIGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLSTTLQASQKKLDELIEQH"
     gene            complement(22143..23822)
                     /gene="ycjM"
                     /locus_tag="OHN27_000536"
     CDS             complement(22143..23822)
                     /gene="ycjM"
                     /locus_tag="OHN27_000536"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCQ2285039.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLVECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLKHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(24035..24349)
                     /gene="pspE"
                     /locus_tag="OHN27_000537"
     CDS             complement(24035..24349)
                     /gene="pspE"
                     /locus_tag="OHN27_000537"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCQ2285040.1"
                     /translation="MLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(24424..24645)
                     /gene="pspD"
                     /locus_tag="OHN27_000538"
     CDS             complement(24424..24645)
                     /gene="pspD"
                     /locus_tag="OHN27_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCQ2285041.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVVLEPLLSRAANKLAQRYKR"
     gene            complement(24654..25013)
                     /gene="pspC"
                     /locus_tag="OHN27_000539"
     CDS             complement(24654..25013)
                     /gene="pspC"
                     /locus_tag="OHN27_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCQ2285042.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(25013..25237)
                     /gene="pspB"
                     /locus_tag="OHN27_000540"
     CDS             complement(25013..25237)
                     /gene="pspB"
                     /locus_tag="OHN27_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCQ2285043.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(25291..25959)
                     /gene="pspA"
                     /locus_tag="OHN27_000541"
     CDS             complement(25291..25959)
                     /gene="pspA"
                     /locus_tag="OHN27_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCQ2285044.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            26126..27103
                     /gene="pspF"
                     /locus_tag="OHN27_000542"
     CDS             26126..27103
                     /gene="pspF"
                     /locus_tag="OHN27_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCQ2285045.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(27223..28488)
                     /gene="puuE"
                     /locus_tag="OHN27_000543"
     CDS             complement(27223..28488)
                     /gene="puuE"
                     /locus_tag="OHN27_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCQ2285046.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(28526..29806)
                     /gene="puuB"
                     /locus_tag="OHN27_000544"
     CDS             complement(28526..29806)
                     /gene="puuB"
                     /locus_tag="OHN27_000544"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCQ2285047.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(29808..31295)
                     /gene="puuC"
                     /locus_tag="OHN27_000545"
     CDS             complement(29808..31295)
                     /gene="puuC"
                     /locus_tag="OHN27_000545"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCQ2285048.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(31570..32127)
                     /gene="puuR"
                     /locus_tag="OHN27_000546"
     CDS             complement(31570..32127)
                     /gene="puuR"
                     /locus_tag="OHN27_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCQ2285049.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(32154..32918)
                     /gene="puuD"
                     /locus_tag="OHN27_000547"
     CDS             complement(32154..32918)
                     /gene="puuD"
                     /locus_tag="OHN27_000547"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCQ2285050.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            33130..34548
                     /gene="puuA"
                     /locus_tag="OHN27_000548"
     CDS             33130..34548
                     /gene="puuA"
                     /locus_tag="OHN27_000548"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCQ2285051.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGNGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            34697..34861
                     /locus_tag="OHN27_000549"
     CDS             34697..34861
                     /locus_tag="OHN27_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285052.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            34851..36236
                     /gene="puuP"
                     /locus_tag="OHN27_000550"
     CDS             34851..36236
                     /gene="puuP"
                     /locus_tag="OHN27_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCQ2285053.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            36370..36615
                     /locus_tag="OHN27_000551"
     CDS             36370..36615
                     /locus_tag="OHN27_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCQ2285054.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            36928..38571
                     /gene="sapA"
                     /locus_tag="OHN27_000552"
     CDS             36928..38571
                     /gene="sapA"
                     /locus_tag="OHN27_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCQ2285055.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            38568..39533
                     /gene="sapB"
                     /locus_tag="OHN27_000553"
     CDS             38568..39533
                     /gene="sapB"
                     /locus_tag="OHN27_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCQ2285056.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            39520..40410
                     /gene="sapC"
                     /locus_tag="OHN27_000554"
     CDS             39520..40410
                     /gene="sapC"
                     /locus_tag="OHN27_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCQ2285057.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            40410..41402
                     /gene="sapD"
                     /locus_tag="OHN27_000555"
     CDS             40410..41402
                     /gene="sapD"
                     /locus_tag="OHN27_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCQ2285058.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            41404..42210
                     /gene="sapF"
                     /locus_tag="OHN27_000556"
     CDS             41404..42210
                     /gene="sapF"
                     /locus_tag="OHN27_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCQ2285059.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            42278..42631
                     /locus_tag="OHN27_000557"
     CDS             42278..42631
                     /locus_tag="OHN27_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCQ2285060.1"
                     /translation="MMDKIKSNARDLRSNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEETV
                     LENILQELNRRSPSP"
     gene            43001..43789
                     /gene="fabI"
                     /locus_tag="OHN27_000558"
     CDS             43001..43789
                     /gene="fabI"
                     /locus_tag="OHN27_000558"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCQ2285061.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            43933..45060
                     /locus_tag="OHN27_000559"
     CDS             43933..45060
                     /locus_tag="OHN27_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCQ2285062.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVDLFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIISQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLEKLLNSTRGNTQPDNQLAALLCARIN
                     GSPACFATCMDSSNEYKKISTLMRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            45128..47062
                     /gene="rnb"
                     /locus_tag="OHN27_000560"
     CDS             45128..47062
                     /gene="rnb"
                     /locus_tag="OHN27_000560"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCQ2285063.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDEMVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(47139..47243)
                     /locus_tag="OHN27_000561"
     CDS             complement(47139..47243)
                     /locus_tag="OHN27_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285064.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            47297..49282
                     /gene="pdeR"
                     /locus_tag="OHN27_000562"
     CDS             47297..49282
                     /gene="pdeR"
                     /locus_tag="OHN27_000562"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCQ2285065.1"
                     /translation="MKTVRESTTLYNFLGSHNSYWRLTESSDVLRFSTTETTEPERML
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIFILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQGFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            49429..49602
                     /locus_tag="OHN27_000563"
     CDS             49429..49602
                     /locus_tag="OHN27_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285066.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            49692..50441
                     /locus_tag="OHN27_000564"
     CDS             49692..50441
                     /locus_tag="OHN27_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2285067.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            50710..50928
                     /gene="osmB"
                     /locus_tag="OHN27_000565"
     CDS             50710..50928
                     /gene="osmB"
                     /locus_tag="OHN27_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCQ2285068.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(51054..51380)
                     /gene="yciH"
                     /locus_tag="OHN27_000566"
     CDS             complement(51054..51380)
                     /gene="yciH"
                     /locus_tag="OHN27_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCQ2285069.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGCKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(51380..52117)
                     /gene="pyrF"
                     /locus_tag="OHN27_000567"
     CDS             complement(51380..52117)
                     /gene="pyrF"
                     /locus_tag="OHN27_000567"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCQ2285070.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(52311..53480)
                     /gene="lapB"
                     /locus_tag="OHN27_000568"
     CDS             complement(52311..53480)
                     /gene="lapB"
                     /locus_tag="OHN27_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCQ2285071.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(53487..53795)
                     /gene="lapA"
                     /locus_tag="OHN27_000569"
     CDS             complement(53487..53795)
                     /gene="lapA"
                     /locus_tag="OHN27_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCQ2285072.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(53944..54708)
                     /gene="pgpB"
                     /locus_tag="OHN27_000570"
     CDS             complement(53944..54708)
                     /gene="pgpB"
                     /locus_tag="OHN27_000570"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCQ2285073.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLTQRICGPLTPPAEENREIAQREQES"
     gene            54878..55468
                     /gene="ribA"
                     /locus_tag="OHN27_000571"
     CDS             54878..55468
                     /gene="ribA"
                     /locus_tag="OHN27_000571"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCQ2285074.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(55532..58207)
                     /gene="acnA"
                     /locus_tag="OHN27_000572"
     CDS             complement(55532..58207)
                     /gene="acnA"
                     /locus_tag="OHN27_000572"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCQ2285075.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELDVNATHKDRQAVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLATAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKGIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(58371..58466)
                     /locus_tag="OHN27_000573"
     CDS             complement(58371..58466)
                     /locus_tag="OHN27_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285076.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(58580..58747)
                     /locus_tag="OHN27_000574"
     CDS             complement(58580..58747)
                     /locus_tag="OHN27_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285077.1"
                     /translation="MVGQEQLESSPLCQDSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(58750..58913)
                     /locus_tag="OHN27_000575"
                     /pseudo
     CDS             complement(58750..58913)
                     /locus_tag="OHN27_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
     gene            complement(59208..60182)
                     /gene="cysB"
                     /locus_tag="OHN27_000576"
     CDS             complement(59208..60182)
                     /gene="cysB"
                     /locus_tag="OHN27_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCQ2285078.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(60393..62990)
                     /gene="topA"
                     /locus_tag="OHN27_000577"
     CDS             complement(60393..62990)
                     /gene="topA"
                     /locus_tag="OHN27_000577"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCQ2285079.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            63370..63621
                     /locus_tag="OHN27_000578"
     CDS             63370..63621
                     /locus_tag="OHN27_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCQ2285080.1"
                     /translation="MNKETHPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(63657..64706)
                     /gene="sohB"
                     /locus_tag="OHN27_000579"
     CDS             complement(63657..64706)
                     /gene="sohB"
                     /locus_tag="OHN27_000579"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCQ2285081.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNARYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            64926..65684
                     /locus_tag="OHN27_000580"
     CDS             64926..65684
                     /locus_tag="OHN27_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCQ2285082.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            65681..66271
                     /gene="cobO"
                     /locus_tag="OHN27_000581"
     CDS             65681..66271
                     /gene="cobO"
                     /locus_tag="OHN27_000581"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCQ2285083.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     TACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(66311..67186)
                     /gene="rluB"
                     /locus_tag="OHN27_000582"
     CDS             complement(66311..67186)
                     /gene="rluB"
                     /locus_tag="OHN27_000582"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCQ2285084.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(67399..69294)
                     /locus_tag="OHN27_000583"
                     /pseudo
     CDS             complement(67399..69294)
                     /locus_tag="OHN27_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(69322..69942)
                     /locus_tag="OHN27_000584"
     CDS             complement(69322..69942)
                     /locus_tag="OHN27_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCQ2285085.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(69939..70820)
                     /gene="yciV"
                     /locus_tag="OHN27_000585"
     CDS             complement(69939..70820)
                     /gene="yciV"
                     /locus_tag="OHN27_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCQ2285086.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            70958..71002
                     /gene="trpL"
                     /locus_tag="OHN27_000586"
     CDS             70958..71002
                     /gene="trpL"
                     /locus_tag="OHN27_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCQ2285087.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    70979..71072
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            71094..72656
                     /gene="trpE"
                     /locus_tag="OHN27_000587"
     CDS             71094..72656
                     /gene="trpE"
                     /locus_tag="OHN27_000587"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCQ2285088.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTAFFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            72656..74251
                     /gene="trpD"
                     /locus_tag="OHN27_000588"
     CDS             72656..74251
                     /gene="trpD"
                     /locus_tag="OHN27_000588"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCQ2285089.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLTERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            74252..75613
                     /gene="trpCF"
                     /locus_tag="OHN27_000589"
     CDS             74252..75613
                     /gene="trpCF"
                     /locus_tag="OHN27_000589"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415778.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCQ2285090.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEEQERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            75625..76818
                     /gene="trpB"
                     /locus_tag="OHN27_000590"
     CDS             75625..76818
                     /gene="trpB"
                     /locus_tag="OHN27_000590"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCQ2285091.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGGPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            76818..77624
                     /gene="trpA"
                     /locus_tag="OHN27_000591"
     CDS             76818..77624
                     /gene="trpA"
                     /locus_tag="OHN27_000591"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCQ2285092.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT        18744 a        20602 c        19277 g        19172 t
ORIGIN      
        1 ttttcgggaa ggattcggtg atatcaatat cccccgcgag gtaacgctta gtggctgcgg
       61 attcctgatt aattggcagg aaggtcactt tttgcagtac cgttttggcg ttatcccaat
      121 aatgggtatt cggtaccacg accagttttt cattgactac gcgatcttta agaacataag
      181 cgccattgcc gatcagattt ccgggtttcg tccactcttt accgctttct acgttggctt
      241 tttgcaccgg aaagaaggca aagttagcgg ttaaattcac aaaccacggc aacggtttat
      301 caagctgaat tttcaaagta tgggcatcaa ctgcggtgac gccaagctgg tctggcgtag
      361 ctttaccatc aataatcgcc tgtgcgttgt tgattcccgc cagcgcggca aaccatgcaa
      421 atggcgatag cgttttcggg tccaccagac gttgccagct gtagacaaaa tcttgcgccg
      481 ttaccggtgt gccatccgcc cattttgcgt tatcgcgcag ggtaaaagtc cagatacggt
      541 tgtcattact tttccactga gtcgcaacgc cggggacaat ctcccctttc tcgttctgat
      601 tcaccagtcc ttcaaacaga tcgcgaatga cctgaatctc tggcaggccc acggctttag
      661 cgggatccag cgacgcaggc tcatctttaa tgtggcgcac cagctcctgc ttctctgcca
      721 gtactgtgcc gctcggaact tctgcagcat acgaaagaga aatgctgctg accaacagcg
      781 cacaacacgt gactgaaaca gagtgcttca taagtttccc tctaaccgac attaaaattg
      841 atgcgtaatt atttgtttcg ccttaaagaa atgcaaacaa cttccatcaa aaaagtgaca
      901 aaggaagcat caaatgtttt aagtatcaat atatcaaatc agatttagtg taaataatag
      961 tctccaaaat gcggtaaagc acatagaact ccgaacacta cgttcgggct tcgcacgttc
     1021 aaacccgttt tcggtatcat aatcagcatc tgtcgccatt gtacgaagaa taaaaaatga
     1081 ccaaaacgtt gaaaggaact gagaatacgt taagagaaga attaaattaa ggtatcagca
     1141 agtagtgcta aacagcacct ttatacttca gcctatctat aaataatgag aatgccgagc
     1201 cagcatgtcg acggtgtgga tagtacaggt gataaccggg taaatctggt gtgaatttat
     1261 ccaaaacacg tatcagtttt ttttctttaa tagcccgctc aacctgatca taaggtagat
     1321 acgccaatcc atgcccatca attgcagcat caatgatcag gtctatcgta ttcagtaaaa
     1381 gctgaccttc catgcgaaca cgaacttcac gtccaccgcg tattaatctc cagcgatttg
     1441 ctgtacccga tgtgggaaga tacaaattaa ttgcctgatg atctattaat tgtgacactg
     1501 acgttggaac acttcggcga gaaaaataat ccggtgagcc aacaatagcc attggtatat
     1561 ctggcccgat tcgaatggcg atcatatctt tatccatctc cccacccaga cggacgcctg
     1621 catcaaaacg ttcagaaacg acatcggtca aaccataatc aatggtgagc tgaatatcaa
     1681 tttcaggatg cgatttcagg aatgtgcgca ttgctggtaa caatatcgtt tttgctgcat
     1741 gttctacagt agtaatacgt attgtcccgg atgggcggtt ctgcagatcg ctcagggatg
     1801 ccatggctga atctatgtca tgcaacatag ggccaagaac agacaaaaga tgctcgcccg
     1861 cttcagttgg aacaacgctg cgcgtggttc gcgtcagaag ccgcaatccc aatcgttctt
     1921 ctatacgacg cactatctgg cttaaagctg actgcgccat gctcaggcgg gctgctgcac
     1981 gagtgaagct acgctcctct gcaacgacga caaacgccat cagatcagcg atttcttcac
     2041 gcttcattca gtatctccat gcaatctatt aatcatttaa atgataggtc taatcatgat
     2101 tttgggtcta atcaacttaa tgatcaatat ctatagtgat atcaccaacc gttaaaacga
     2161 accgacaact ccatgaggaa cggaatgatg aataataaag tcagcttcac taacagcaat
     2221 aatccaacca tctctttgtc tgcagtgatc tatttccctc caaaatttga tgaaacccgt
     2281 cagtatcagg caattgtgtt atctcaccca ggtgggggcg ttaaagaaca aacggccgga
     2341 acctatgcca aaaaactggc ggaaaaggga tttgtcacta ttgcttatga cgcatcttat
     2401 caaggtgaaa gtggcggcga gccgcgtcag ttagaaaacc catacattcg tactgaagac
     2461 atcagtgccg tgattgatta cctgacaacg ctttcttatg tcgacaatac ccgaattggt
     2521 gcgatgggaa tttgtgccgg tgcaggatat acagccaatg ctgcaattca ggatcgacgc
     2581 atcaaggcca tcggtaccgt tagtgcggtc aatattggtt cgatattccg taatggttgg
     2641 gaaaacaatg tgaaatctat tgatgccctg ccatacgttg aagcgggttc aaacgccaga
     2701 accagcgata ttagcagcgg agaatacgcc attatgccac tggcaccaat gaaggagtcc
     2761 gatgcgccga acgaagaact gcgtcaggcc tgggagtatt accacacccc tcgcgcgcag
     2821 tatccaacag caccgggtta cgctactttg cgcagcctta accagattat tacctatgat
     2881 gcttaccata tggcggaagt gtacctgact cagccgacgc agattgtggc gggcagccag
     2941 gcagggagca aatggatgag tgacgacctg tacgatcgag catcaagcca ggataaacgc
     3001 tatcacattg ttgaaggcgc aaaccatatg gatctttatg acggcaaagc ctatgtggct
     3061 gaagcaattt cagtattagc tccgttcttt gaggaaactc tgtaatgaaa catattcaaa
     3121 tccgcaatag tgatatggcc tggcatattg ctgccaataa tctgggataa ttttcatgaa
     3181 aaatgtactg attcttggtg ctggcggtca gatagcccgc catgtgatta atcaactggc
     3241 agataagcaa accattaaac aaactttatt tgcccgtcag ccagcaaaaa tccacaaacc
     3301 ctatccaact aataaaatgc aaacaacttc cggcaaaaaa gtgatacaag atcgatgaca
     3361 tagcgttgaa gaaaaggatt tgataatccg caaaatttgc acaacactgc ctgtaatact
     3421 tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg gcgcatttcc
     3481 gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct ggtttcctgc
     3541 ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg atgaaaactc
     3601 ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc gccgtcatca
     3661 tgtggtgctg tgtgtgaatc ctgacggctg ccagttgggg ttacgggcca atgctaatgg
     3721 tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa ccgtttatcg
     3781 ctggaacagc gccgctgaag aacgcgatgt cgtattactg acaggtgaca aacctggctc
     3841 cgaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg cctgggtggt
     3901 ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat taggcgaatg
     3961 gctggctcag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa cgcctggctc
     4021 tttcggcagt tggtgtgcag atctgaactt gcattgcatc accgcagagt ttccgcccat
     4081 ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc tgcgctggca
     4141 tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc taccgccagc
     4201 caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa agcggcgcta
     4261 atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg tgcacgcgct
     4321 tcagtcgcca gcgccagcgc ttccgtcaga cccccggttt tatcgagctt aatattaacc
     4381 atctcatagc gccctttcag cgccttcaaa ttgctacgag tatgacaact ttcatcagca
     4441 caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc aggaagcggt
     4501 tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc caacccttct
     4561 gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc tgtgcgaatt
     4621 gccaccatcc gctcactgat aagatggtta tccagcttca ctttcagtaa tttcgcgcct
     4681 gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat gacaaccgtc
     4741 tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag cgattgctgc
     4801 tgtcttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc tgcgccggca
     4861 gggagaattt tttgcaactc ctcccgtgtc agccctttct ctagttgcgg cacgacgctc
     4921 ataatttgcg ccattaccga ggcatcactt tccccataac gcggatacgg cgtgcattcg
     4981 ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc ttcactgcga
     5041 cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcgaa taccttaacg
     5101 gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa atagcgtttg
     5161 ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta aacaggaaag
     5221 ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc caattccatc
     5281 ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct gtctgacgtc
     5341 accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag tattgatacc
     5401 ggtgtttgcg ccgcatcagt acgtaagttt aaccaactgg caaccgagat cgacaacacc
     5461 gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa
     5521 ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct gcaagcctac
     5581 ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt tgtggttatt
     5641 gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac cgagccggat
     5701 tacgaagcag ctctggctgt actgaaagca taatttaata tgcctgatgg tgttgcacca
     5761 tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat tcccgcaact
     5821 tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc gtgctgggat
     5881 aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt tcgtccagcg
     5941 aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc ggcaacaaaa
     6001 tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag atagcgttct
     6061 ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc aggtcagcag
     6121 ccagtttcgg acgcggcacg ccctgctcgt cggcaaagcg ggcgacgaac agctcagtta
     6181 acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc aacacgttca
     6241 gacgataata gagatcttca cggaacatac ctttttgcac cagttcgacc agattcttct
     6301 gcgtagcgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca acccgacgga
     6361 aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac atttccccta
     6421 tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc ttcccttccg
     6481 gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca cagttcagcg
     6541 ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca aagagatctt
     6601 tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc agtttctgcg
     6661 cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg aaggcgctga
     6721 cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat cgcaacatca
     6781 ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca ggcgtaatct
     6841 ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct tgcggttcgc
     6901 tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta tggttgcgca
     6961 ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata tccactttgc
     7021 ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg ctcaacgcca
     7081 gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca cccgcaatac
     7141 ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca aaattgaggt
     7201 agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt agcacgagta
     7261 gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc agacgcatgg
     7321 gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt ggatagcagg
     7381 aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc ggcgtaaaga
     7441 tatccggtca gcacccgata ttgcattatt tttcgctggg cgttttgcct ttttgcagcg
     7501 tttctttcac ctgaccgata agctgacgac ggaaatcccc gaggcgaggt ttgtcatcgt
     7561 caatccacgg cagcgggcgg caaagctcca tagctttaat cccgagtcgt gccgtcagaa
     7621 gtcctgcacc aatcccctga gctgcgcggg tagacaaacg agcagcgaga tcttgcgaca
     7681 tccagtccat ccccacttcg cgcaccagtt cgctggctcc ggcaaaagcg atattcagca
     7741 ataccagctt aaacagacgc aaacggctgt aataccccag ttcaatgcca tacagcgtgg
     7801 cgatgcgatt aattaaacgc agattgcgcc aggcgataaa cgccatatcg accaacgcca
     7861 gcgggctgac cgcaatcatc aacgttgatt ccgccgccga acggctgatt tcgcgccgcg
     7921 cctgggcatc taaaactggc tggaccaaat gcgcatacag gctgaccact tcacggtcgt
     7981 tttgcgtttc atggattgag gcataccagc gttgcagcgc cggatgcgac tgatcaatac
     8041 ccgcctgctg cgccagtttt tcgcaaaatg cgcggccttt gcccgtgccg tggctatgca
     8101 ataaatcacg cgcttcgtcg cgttcatggg cgcgctgtcg caagcgccat aagcgccgcc
     8161 actctgttac cacagaacct acgccagcgc cgataatcaa tgccccagcg gcacatccac
     8221 ccagcgccac ccagtcctgg gtttgccagg cattcattgt ccactgtaca ccctgcccga
     8281 caacgcttgc gccaaacaga gccagcccgc ccatcaccat tttgcgccac aggctgcgtt
     8341 tcggacgtaa cgctgcgtcc attaccgctt cgacttgccc ttcttcttcc tgcgcttcgt
     8401 cgagcgtggc cggggcaaaa ttttgcgcct gattttcgtc aaaggtctgc tgcgccctga
     8461 atttaggatt ctgatcgacc tccagaggac cgtcgaaatc aatacgtggt tttaacggtt
     8521 cggtcatcgc aatttatctc ctattaaaaa ttccagcgca gcatcaagac gaatatgcgg
     8581 tagtggtttg tcgacatcca tcacctgcgg acgaaatgcc tcaaactgga agccttgctt
     8641 atcccagaac gcctgaccag gcaaacgtgc gggaacttcg ccaggataaa cagtgagcgg
     8701 tgcgccatcg ctaagtcgat taccacgcag cgccgggatt ttctcaccgt taacatcaat
     8761 aatgccgctg gtggtcgcct gaactgacgc cagccccagg cagtccatgc tgatcccttc
     8821 gaacgccgca ttttgccagg catcctgaat cagttgttgc agtaatgaaa ccatattagc
     8881 gtgctgatcg atggtcacat ggtccgcttt agtggcagca aacaatagct tatcgataac
     8941 cggcgaaaac aaacgccgga acagggtacg ctgcccgtag tgaaaacttt gcatcagctg
     9001 cgtcagtgcc agacgcatat cattaaatgc ctgtggccca ctgttgagag gttgcaggca
     9061 atccaccagc acaatctggc ggtcaaagcg cagaaaatga ttcttataga accccttcac
     9121 caccttctcg cagtaataat taaagcgctc gcgcagcatt ccggcattgg tatgcttatc
     9181 ggcctgcgcc agtttggact cgccccaggt atcgacatcc ggccacggga agaattgcag
     9241 cgcgggcgca cctgccatat ctcccggcaa gacaaagcgc ccaggctgaa taaagtgcag
     9301 cccctgctct ttacagtggt ggagataatc ggtccacgcg gcggcaatgt ccgccagccg
     9361 gttttcgtcg gcaggcgcta gcgggtccag cccttcgctc atcattcgcc atttcgccga
     9421 ccattctccg cgctgaccat tgagtaagcc cgtcatctgg cgcgaccagc ttaaatagtc
     9481 ctgcgccagc atcggcaggt cgagcaacca ttcgccggga taatccacaa tttccagata
     9541 cagcgtggag gtatccttaa agtggcgtag cagcgaatca ttcgatttaa aacgtagcgc
     9601 caggcggatt tcactgacgc cgcgcgttgg cgttggccag gcgggtggat cgccatataa
     9661 ctgcgccagc ccttcgtcat aggtgaagcg cggaatgcca aagtcacgct ggggaatgcg
     9721 tttcacgccc agcaggcgct cttcacgcac cgcacttaac agcggcaaac gtgctccggc
     9781 gtgaatatta agcaactgat taaccatcgc ggtgataaac gctgttttgc cgctgcggct
     9841 aagtccggtt acagcgaggc gcagatgtct gtcgacaccc cgattcacca gcgcattgag
     9901 ttcattttta agtcgcttca ttgctgtcct gtgctgctct gaagaccaga aaaaaaatca
     9961 ttcgtaagga tatgaatgtg ggggccaggc cattgattat caactgcccg tgttgtcatc
    10021 gaccggcgaa ctccataaga tgtgcagcat tcacgttcgg acggagttcc tgacctatgt
    10081 cccctactat ttatgatatt gccagggttg caggcgtatc aaaatccacc gtatcacgcg
    10141 tgctgaataa gcaaactaat atctccccgg aagcgcgcga aaaagtgtta cgggccattg
    10201 aagaattaca gtatcagcca aacaagctgg cccgcgcgct gacctcttcg ggttttgatg
    10261 ccattatggt gatttctacc cgttcgacca aaactacggc gggtaatccg tttttctctg
    10321 aagttttgca tgccatcacc gccaaagctg aagaagaagg tttcgacgtg atattacaaa
    10381 cgtcgcacaa tccggcagaa gatctgcaaa aatgcgaaag caaaattaag cagaaaatga
    10441 ttaaaggcat tattatgctc agttcgccag cggatgagtc attttttgcc caactcgata
    10501 aatatgatat tccggtggtg gtgattggca aagttgaagg tcaatatgcc catgtttatt
    10561 ctgtcgatac cgataatttt ggcgacagca ttgcgttgac cgatgcatta attgaaagtg
    10621 ggcatcaaaa tattgcctgc ctgcatgctc cgcttgatgt ccatgtttcg gttgatcgcg
    10681 ttaatggtta taagcagagc ctggctgcgc ataatattgc agtgcgtgat gaatggattg
    10741 ttgatggcgg ttatacccat gaaactgcat taaaagcagc acggcaatta ttaagccagt
    10801 caccgttgcc tgaggcggtg tttgccactg acagcctgaa attaatgagc atttatcgtg
    10861 cggcagcaga gaaaaatatt gctattccgc agcagttagc ggtggtgggt tatagcaatg
    10921 aaacgctgtc atttatttta acgcctgcac cgggcggcat cgatgttccg acgcaggagt
    10981 taggtcaaca aagctgcgag ttattattcc gcttaatttc tggaaaaccg tcaccacaga
    11041 atattaccgt tgccacgcat atgacgctga aataaatacc agctaccttg cctgaatgcc
    11101 ggatgcgatg ctagcgcatc ttatccggcc tacatttata gatcggataa agtactcgcg
    11161 ccgcatccgg caatttagcc cattatcaga acgagtaatt tacgccgaca cctgcataat
    11221 ggaatttatc actgtcgcct tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa
    11281 cggaaagtcc gttctggaaa tcataaccgt aaaataaacc tacacggtta aaatcatggc
    11341 cttcacgttc aatatcatcc tgccagtccc agttactcca gcgatccagc ccaatgcgcg
    11401 tatacggcgt caccgagtgg ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg
    11461 tggaaaactc accgttattg cggctgtcgt ccatattgaa gccgcgctcg agataatagt
    11521 tcactcgcaa ggcaaccgtt tcgttgaagg tatattgcag acctgtttcc gtttcgacac
    11581 gggtatcagc gtaaccggta gtgttcagat cgttggcaaa tttatacatc gacaaccaac
    11641 cgttgaaacg taaatcgtca gtcagtttca catcccagtc tggcgcgatt ttccagcgct
    11701 gcatattcgc cgtgtcttta cccggttcat caacgtagtg ataaccataa ttacggaaac
    11761 cgccggtcag gccgaaactg aaatcatcgt tttcgaggaa ctgataatgc acctccagct
    11821 ccgggcgatc aaaccacgtt ccacgtttac ccgcgctata atctaccggc ccttcctgat
    11881 aataggccag agcaattctc cacggcccgt tggcggcatt aaaatagact gaaggctccg
    11941 ccagcccatc catatcttcg ccataaccct cgacgttttc tatttcgtac atcgcgccga
    12001 tattaaagtg ccagtcgttc ctttcctcgg cctgtgcgca ggccattccc gcacacatca
    12061 ccagtgcggt acagggtaat aactttttca ttattgttcc ttgtattatc ctgggggaat
    12121 gccttccccc tgtatttagc gaattgctat ttccgtttct gcatcaaaga aatgacattt
    12181 cgtcatatca aaatgaatag tgatattttc tcctgcatga taatcattta acgcaccagc
    12241 acggaccact aactcgtgcc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    12301 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    12361 ttccggtcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    12421 taatttctct tcgggaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    12481 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggaaacaa acatattcgc
    12541 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    12601 cacaatccgc gtcgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    12661 ggtgttcagt ttctgatgca gcttgctgat ctctgcgcgc atttgcacgc gcagcttggc
    12721 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgta caatcgcccg
    12781 cccaagcgcc actcgctgac gttgcccgcc ggaaagcgcc cccggcttac gtttcaggta
    12841 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    12901 gattttttgc atcttcagac caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    12961 cgcgtagttc tggaacacca tcgctatatt gcgtgctttg gctggaacgt cattcattcg
    13021 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    13081 ggtggtcgac ttaccgcagc ccgacgggcc gacaaacacg atgaactctt tatcggcaat
    13141 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    13201 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    13261 gcggccaggt gagtgattcc gttgaaggca acagtaattg cgccccggtt aagcccgcgc
    13321 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    13381 caatgccgat acatgcctgc ggcggcacgc ccagccctgc acaggcggcg agaaagattt
    13441 ccgggtccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcgc
    13501 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacagaagcc agcccaaccg
    13561 agatctgctg tgcacggaga tctgccagca aagagcgaat gccgggtaga acagcgttga
    13621 ccgtcaactc gcgtagtgag tggacataga gcagattttt gcgatacgcc agttgcgccc
    13681 tctcctgcga gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    13741 catcgcggct gatccctttt agggattcgt taaactgcgc atcaatgctg atgccaattt
    13801 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    13861 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgtttgg tagcggtccc
    13921 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggttataag
    13981 ctgaccgttc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    14041 aagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    14101 ctcaggtaac gccggattga gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    14161 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    14221 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    14281 cagcaggcca cagcgtgcgg caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    14341 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgctg agaactgctc
    14401 cggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    14461 ttctgcgcgt gaataatcca gcagtatggt ttgcttcccc gccgccgctt tgtatttcgc
    14521 cagattaatc gccggcttag ccataaacga atcatcctgc ggcaaaacgc cgtcgggctg
    14581 aatttctggc atccatagct ctttgaggaa catttcggcg cgatggataa acgcatcgtc
    14641 gctacagccg aactggcggg caatattcag cgcctgttga acgttgtagc gggccatata
    14701 gctggtgtat gcattattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    14761 aatttccaga cgatcgttaa ctctcaccgc gcggctaatc cagaactttg ccgtctccag
    14821 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    14881 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    14941 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    15001 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    15061 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    15121 aaacagatga aacggtaaca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    15181 ttcgccggtt aagccttttg ccgcaatgct gctgcgctcg tcgtgggcag gcgtcatgat
    15241 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccgtttac
    15301 cgtgatacga cgtttctgcc accattgacg ccagttttct gttgatgctg caagaagttg
    15361 gtcgtaactc tgttgcgcac acatttcaag ctggcgaagc gacgcgctgc cccactcgtc
    15421 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    15481 ctcgcctgca tgaagctgcg cactggtatg ttgcagtaaa cggcgctctt tggcggtata
    15541 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    15601 atcctgggtt gtgtagctcc cctgcatcag atgctgacca aacacccgca cctgggtttc
    15661 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    15721 tgacgcgtcg gcgtccagtg gcgtaatagt gatttccagc gcaatgagcg gcagttggtc
    15781 tgccgaaaca aagcgacggc tggcgatggt gtaacctgtg ccgttgctgg tacgccagac
    15841 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    15901 taacgagaaa acctcaccat ttatggcaat ctccatcccc acgacatcag gcaggttcac
    15961 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    16021 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    16081 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    16141 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata caacgccgcc
    16201 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    16261 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    16321 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    16381 gcaccagctt tatcaccaca aaagctgacg ttcataatcg actgttcgcg gatgttgagt
    16441 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    16501 gaatcttcga cgctgtaagt tgccggatcc cactcaccaa attgaccaca gctcttttgc
    16561 gtgccgatct tttgaaagct atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    16621 tacatcgcag catccagcat atgaatgccg atgtcgatca gcgggccacc accctgcagt
    16681 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    16741 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    16801 agcgcaaaac gatggtgaaa gtcgtaggcc agcaccttgc ccagtttgcg cgcggtatcg
    16861 cacatttccc gcgcctgttc tggcgtcatg gcgggcggtt tttcgcacat cacatggcag
    16921 cccgcttcca gtgccatcag ggtatgttcg taatgaaaac ggttaggtga gcagacgcta
    16981 accacatcag gtttcaccgc cagcagcatg gcctgcggat cgtcccacac ggatgcattc
    17041 ccgtattttt ctgccagcgc ctgcgcctgg gaaaggcggc tgtcacagac agccaccagt
    17101 tccagatcgt tgcgggtgca gtagtacgaa gcatgaacct tatccgccac ctggcctgcg
    17161 cctattatcg cgacccgcag cggagagctt gtcattgcac ttttcacaaa gacctcttag
    17221 caggtacgca accaggccaa cgaatcacgg tacgcctggg caggatcttc cgcccggata
    17281 cgcccttcgt acactacata gccctgatag ttatccgcgc gcagctgttc aaacagcgcg
    17341 tggaaatcca gggtgccgct gcccggctgg tagcgatggt tatccgcaat atgcacatga
    17401 ccgagcaggt cgcggttgtc atgcagcgcc tgcgccaggt tatcttcttc gatattcatg
    17461 tgatagaaat cgccgataat ctgtacatgt ttaagatcgt tttcgacgat gtaacggcgg
    17521 gcatcggcga gggtgttgat catatgatcc tgatagcggt ttaacggtcg agatacacca
    17581 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accattttgc
    17641 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    17701 acgcagccgg aacgacgatg cctttaccgc ccacttctgc cagcgcttcg agaatgcgct
    17761 cgatctgctt taagccatta agacgacgct cttcgataaa gtcgccaatc catccgtcat
    17821 agccaccgca ggcggtggtc accggtaaac cggtttcttt gatcgccgct ttgacttctt
    17881 cgatgttgtt aaccagcagt ttgccgtcaa tctcaaaacc atcgaagccc atctctttga
    17941 tataacgaaa tttctccaga atgtttttcg gaaagaacgc ctgattctgt gtgccgattt
    18001 tcattttttt cattcctttg aattaaaacg taacgcccat tttgatgctc tgttccggat
    18061 gctggtcgac atactgcata tagctttccg ggctattggc aaaggtcacc accgggtcga
    18121 ttaaatcttc gcaattgaga taaccgttca tcagcagttc ccaacaggtt tcttcaatac
    18181 gcttgcggct ccagcgcgga taatccgggt tcggttcgct gcacgcgcga gagaagacaa
    18241 ttttggcgtt attgaaatgc gcttcgcgtc cgaggttaaa accttcggca aacggcttgg
    18301 caaacgcgac ataggagatg gtgccgccat aagccagacc gcggagcgcc gattgcagcg
    18361 cgtcggcgta gccgctggtt tcgataatca catcggcacc ctgcttgccg gtcagcgttt
    18421 tgatctcttt acctacatca gtgccgatgg ggttaaggca gaaatccgcg ccgtggcgac
    18481 gggcaatatc acagcgatgg gcgataggat cgacgccaat caccacagaa gcgccagcgc
    18541 gtttagccag ttggatggca atttgaccga ttgcgccaag ccctaccacc accacaaaat
    18601 cccctacgcg cacgttggca tcgcgcacgc cgctcatggc aaactgcgcc gggtcgtagc
    18661 agacggcgtt tttccaggag ctgccttgcg gcattttgcg cagcttgtag ttattcactg
    18721 cgttaatgat gaccgtctcg gagagcgggc cgtagccgca tacgctgtcg cccaccgcgt
    18781 agtcggtaac gtcgctgccg cactcgataa tgtcgccaac caccatgttg ccaagctgga
    18841 atttgccaaa ctcaatgccg cgcggcgcat ctgccggacg cggagtgaac atctgccatt
    18901 cgccgttaaa gtcttcatcg ataaacgggc tggcggcgcg gaagtcgacc acttccgttc
    18961 cgtgtttcgg tgcgccgaaa cgcacgcgga tcttcacttc attagctaaa atcgcccgat
    19021 cttcatactc aaccagcgca gcaacacgcg gtgctgtggc tactaacttt ttcatttaaa
    19081 actccttgta aagaaactgg ccgctttagc ccttcacgcc accggcggtc aaaccactct
    19141 taataaaacg ttccgaaagc gcatacataa tcaccaccgg caatgcggtc accagtgagg
    19201 ccgccatcat ccgtccccag atgtaatctg gcgtactgaa cagcgcgttc aggcccaccg
    19261 gtaaggtgaa attgctggcg ctggagagga aaatcgacgc aaacagatag tcgttccacg
    19321 ccaccataaa gcagtagacg aacacggata tcagcccgga catcgccaac ggcacggtga
    19381 tgcggaagat aatttgcaga cggttgaggc catccatcat cgccgcttct tcgatctcat
    19441 cggggatggt gtcgaagtag cttttcagca taaacacggc ggtgggcaga gtttgcgtca
    19501 ccatggtgat gatcagcgcc atctcagtgt cgtaaatgcc aagcgcggtg atgattttga
    19561 aaagcggcac caccagcaaa atgccagaga acatatacac cgtgtaaaag ctggcgttga
    19621 tggtcatccg acctttaaag cgcaggcggg aaagcgcgta agccccaaga atgccgagga
    19681 ataccgccac cacggatgaa accacagaca ccaccagact gttacggaag tagtcgacaa
    19741 acgggaaaat catcgggtta aaaatgtcga cgtaatgctc cagcgtccac tgctgcggca
    19801 gcagcgtagg atgcagtgag atcgcctctt tcgcgccctt gaacgaggtc atcagcatca
    19861 caaaaaatgg aaacagcgtg atgatgagaa acagcgccag cccgcagtaa aaaccgatgc
    19921 gactgagtgt gcgtttattt gttgccattg aggttcaccc gtttcctggt cagcagaatg
    19981 acagcgaaaa taatgatgaa gagcaccacc gagatcgccg ccgcttttcc taaatcatta
    20041 aaggcaaagg cggttttgta gagatataca ccgagaatat cgaccttggt cgtcagcaaa
    20101 taaacatccg cgaacatgta gaacatccag atggtgcgca gtgtcaccac cgtcgccagg
    20161 accggcataa ttgcgggcag cgtgacgata cgaaaccgtt gccaggcatt agcgccatcc
    20221 atctctgccg cttcgtacag cgatttgtcg atggtctgca aaatcgccag aaacgagata
    20281 aaggcatacg ggaagtagcg ccagatggcg aacagcacca ccagcacaaa gctactgccc
    20341 ggattatcga accacagcgg tgcctgctca tagagatgca gaagatcgac gccgaggtag
    20401 ttaacaatgc cgtagccgtt gttgaacatg tatttccagg cgaacaccag cgaaatggac
    20461 ggcgttacgt aggagaggat caccagcgaa cgcgcggttt tgcgcaggcg gaactcgcgg
    20521 ttgaaaaaca tcgccaccgc cagcccgagc acggtgctgc ccgccaccac cagcgcggta
    20581 taccagaccg tcatccacag cgaatgccag aagccgggat cggagaggat acgcacatag
    20641 ttgctcaccc caacaaacgt tgactcgatg ttgggattga gcggcagacg taaaaaactg
    20701 atttcgatat tcgacaccat cggccacgcc accagaccgc ccagcagtaa taagctgggc
    20761 gcgagaagca gcagcgcaaa gggcatatcg gaacgacctg aaaaaagcct gttcattgcg
    20821 gattcctgtc ctgggtttag tgctgttcga tcagttcatc cagttttttc tggctcgctt
    20881 gcagcgtagt agagagatcg gctttaccca cggtgacgtt atgcaccatt gaactcacca
    20941 cgccagaacc cgtcacatca cccatgcggg taaagttttt atcccctact gcgccaaaaa
    21001 cctgaatatt tggcagttca ccgattagct gattcggtag ttcacccagc gccttaataa
    21061 cgtcgttgtc tttccaggtg gcggtagtca ccaccgcttt attcaccggc agcgcagcac
    21121 ctggcgacat catcacccag tcggcaatgt tgtctgcctg ctccataaag gtgacaaatt
    21181 tttctgctgc ttccgtctct tcggtctttt gcccggcggt aatggtcagc gaggtcaaca
    21241 tgccgtagac cgcagagttt ttctcggttg gcaccacgaa accgacgttt ttcgggtcgc
    21301 cttctttaat cacagccgga aggatatagg tggagtaaat cgccatcggc gcggtgccgt
    21361 tcataaaggc gtctttcact tccatgatgt cgttagaacc cggcatagtg ttggcagcaa
    21421 ggtcgcggta ataggtcagt gcctgcatca tctctggtgt atcaagggtg attttgcctt
    21481 cggcgttaaa gacgttagcc tggttggata acgcaaactg ggagaaggat tgttccgtca
    21541 acacgctttc tgctgtaggc agcgcaatgc cgtatttttt attcgccggg tcattcagtt
    21601 tctgtgcaac gtccagcagc tgttgccagt ttttcggctc ctcaagcccc gcttttgcca
    21661 gcacatcttt gcgataccag ataccgccaa tccaggcgct gacaggaaca ccgatccatg
    21721 cgctaccatc ttcggtacgc acaatacgca gtacgccatc gtaaaacgcg ccttcaccaa
    21781 cgttgctgat gactgtggca accgctttgc gatcgataag ctgctctttg tccatcactt
    21841 tggcgtagtc atggctggtt tcgatcactt ccggcagcga accgctacgt gaaagagtaa
    21901 tgactttagt gttataggca tcttcttcca cgggcacttg cttaacggtg atgccagggt
    21961 tttctttttc aaaacgggca atcaatttac tgataacggc ctggcgctcc tgctccaccg
    22021 aagaatgcat aaattcgatg gatacattcg ttttattttc ttctttacag cctgaaatca
    22081 gggcgcagga aaccagtgct gataacagca cgatttttga tttaatcatg agatgttcct
    22141 ttttatttaa tccacataac ctgccagggg cgcagcgtta tttcattacc caatatatca
    22201 acttcactaa tcagatcccg accatgcaga ttagtaatat ttacatgctg aatattttta
    22261 ctgacattaa acaatccagt cagacaatta ccatcagcgt tacttctttg aatacgcatt
    22321 acggatgaat taatcgtatc aatggtaaaa ttattatccg gatgaaactc gttatggctg
    22381 cgacgaagtg taattaaacg cgacaactca tgatataccg catgctttaa tgtagcttca
    22441 tcgttcagtt ctcgggttat ctctttactg tgatattttt tacggttaat cgcacggtta
    22501 tatccgagtt tttcgacacc tgcgtaatca ttacgcgagc caagaatact ttgaatatat
    22561 atcgctggca caccggggaa acttaacaaa atcgcatggg cgagaataaa cctggcgcaa
    22621 cgttcttcat cgctactctc acggcggctt aacgcatcca tataggtcac atttatttca
    22681 tacggactgc gtgtaccgtc gggattattt ttccagttta ctaatgcacc ttcctgctgt
    22741 aacgcctcga ccagctctaa tatttcgctt tcaggcaaca agccccgtag cgggtttagc
    22801 ccgatgccat cgtgagaggc gaggaagtta aaccaggtgg tgttgctgga aggtagtgtc
    22861 aggttttgcg cccacgcaca aagcgcctca acgttctgtt tttgcaccgc atgcagcacc
    22921 agtggcggca gcgagaactg gtacaccata tgtgcttcgt catcgcctgc gccaaagtaa
    22981 gcaatgttgt ctttatgcgg aacattggtc tcggtaatga tcactgtacc tggcgcaacg
    23041 ttatcaataa tcgaccgtaa cagtttgata atcagatgtg ttttttccag atggatgcag
    23101 cttgttcccg gctctttcca cataaagcca acggcatcca ggcggacata ctcagcacct
    23161 ttcgcaaggt aacagagtaa aacatccacc atcgccagca acacttcagg gctacggtaa
    23221 ttcaggtcaa tttgatcgtc actaaaggtg gtccataaat ggcgcgttga atgatcgcgc
    23281 atctggaatg gcgttaataa cggtaacgca cgcgggcgag tgacggcgct gagatcggtt
    23341 tgcgggtcaa cggcaataaa aaaatcttca aaacctggat gctgttgtaa atagttttta
    23401 aaccattcac tttttgccga catatggttg cagacaaaat caaacattaa atgactgcat
    23461 tcaacgagtt gctgaatatc ctgccactcc cccgcttcac tggcgacctg atgataatca
    23521 attaccgaaa agccatcatc agatgaccat ggataaaacg gcaacaaatg aacatgtgaa
    23581 aaaatgcttt gcagccattg atggtaaaac tgattaaatg tgggtaatgg ttttaaatca
    23641 ttgctgtgaa attgatcggc ataggtaatt aacacgacat cactttcatc ccagtgtttt
    23701 ttacgtcgct gtgtaattaa tcgtttcgca ctctcaagac gcgttacaag tttctgtaaa
    23761 tgagttgcgg taaatgttcc accgtagatt tcgtccaggt aatccgtaat tttctgtttc
    23821 atgttgcctt tccgtggtag cggtcccatg aaacgagaga ctactccgca gaaaataaac
    23881 tgtcaattga gcacaagggt tttagtcacg caggaaatca ttttttacca gggaaaaagc
    23941 gtatttgtga gctgacgcaa ataatctatg gggatgtaaa taaggtgtga cagaaaaaaa
    24001 aacggcgcat aagcgccgct catggtgaat tcttttaacc tttgaccttc ggcattgcga
    24061 tgtctttcag gccaccggcg ttctcaacgt gggtatatcc catctcgcta aggatctctt
    24121 ttgcttgccc tgactggcgt ccggcattgc aatacacttt cacggtgtcg tttttatccg
    24181 gaacggcggt ggcaatgcgc tctttcactt ccttcagggg aatattgatg gccccctgaa
    24241 cgtgctcttg ctgatactgc tctggaacac gaacatcgat ccagtgttca gcggcgaaaa
    24301 cgggcagtga aaacaccagt gccagagcaa gtaagccttt ttttaacatg taagcgtcct
    24361 tctatggatg aaaattgcca tccattatag gcctgagatt taacaatttt tgcgaaactc
    24421 ccctcacctt ttataacgct gtgccagttt attagcagcc cgacttaaca gcggttccag
    24481 caccacagcc agcaacattt tcagcggtcg gcgagcaact gattttatcg cccagcccgc
    24541 tacacccgcc gggccatagc gcagtgcggt aagaagtacc agcttgcctg ctaatttgaa
    24601 accaggcttt accttttgcc cggcctgttg ccagcgagta ttcataactt tcctcacagt
    24661 tgacggaaac ggctacgtaa cgtgaaagta tcggaagtga cataacgttc catctcgcgt
    24721 aaacgcgttt cacttgccgc cagttcacgg tcgacttcat ccagcaattc gctgctggaa
    24781 ggtagctgct caccaaaggc catgttgtcc ggcattggat caagcgcaaa tgacaaaatg
    24841 atgtaagcaa ccagggtaaa cagcgccaga ccgaagaaaa tcgacagcac caccaggata
    24901 cgcaccagtt ttaccggtac atcaaaatag ttggcaatcc cggcgcagac gccgcggacc
    24961 atgccctgct gtggaatacg ccataatttt ttattgagat taatgcccgc cattagcgat
    25021 ccctccagtt cggatgttct gcatcaagaa tagattccag cgcctgaata cgttcgcgca
    25081 tccgttttgc ttcatcagcc agttgcgcta atcgctgctg ctcactttgc gacaattcac
    25141 tgcgaccaga acgattgctg taatgcagcc ataaccagat cggtaaaaca aacagcacaa
    25201 aaatggttaa cggaatagcc agaaatagcg cgctcataag tactccttag acgatgagcg
    25261 gcggcgcgtt cggacgccgc ctggatgtta ttattgattg tcttgcttca ttttggcttt
    25321 taattgtgcc agttgctcgc tgattgcatc atcggctttc agttcggcaa actgatcgtc
    25381 cagcgatttt tgtttaccga agctgtggct ttctgcttcc gcttccatct ggtcaattcg
    25441 acgttcgaaa gattcgaaac gagccattgc ttcatccagt ttgccactgt ccagctgacg
    25501 acgcacatcg cgcgacgagt ttgccgcctg atggcgtaac atcaatgcct gctggcgagc
    25561 gcgtgtttcg ctcaatttgt tttccagctc gccaatctct ttcttcatgc gtgccagcgt
    25621 atcgtccacc agcgtcactt catgttccag ggacttaatc agatcggtca gtttctgttt
    25681 ttcaattaac gccgcacgcg ccaaatcctc tctctctttc agcagcgcca gttcggcttt
    25741 ttcctgccat tcaacttcac gcgccgacgc ttgttcaata cggcgagtca gctgtttctt
    25801 ttctgccaac gcgcgcgccg aagtagaacg tacttcaacc agtgtatctt ccatctcctg
    25861 gatcatcaga cgaaccagtt tctgtggatc ttccgctttc tctaacagag cgttgatgtt
    25921 ggcattcacg atgtcggcaa agcgagaaaa aatacccata atgttgtcct cttgatttct
    25981 gcgttcagga ttgtcctgct gaactgttaa tacaatttgc gtgccaattt tttatctttt
    26041 tgatttataa agatctgatt gaagaatcat cagcaacatg ccaggatgaa ttagcgaatt
    26101 acactaacaa gtggcgaatt tcatcatggc agaatacaaa gataatttac ttggtgaggc
    26161 gaacagcttt ctcgaagtgc tggaacaggt ttcgcatctc gcaccgctgg acaaaccggt
    26221 gctcatcatc ggcgaacgcg gcaccggtaa agagctgatt gccagccgcc tgcattatct
    26281 ctcctcccgt tggcaagggc cgtttatttc ccttaactgc gcggcgttaa atgaaaatct
    26341 gctggattcc gaactgtttg gtcacgaagc gggggcgttt accggtgcgc aaaaacgtca
    26401 tccagggaga tttgaacgtg ccgacggcgg tacgctattt cttgatgaac tcgctacggc
    26461 accgatgatg gtgcaggaga aattattgcg cgtgattgag tacggtgaac tggagcgcgt
    26521 tggcggcagc cagccattgc aggtgaatgt gcggttggta tgcgcgacga atgccgatct
    26581 cccggcgatg gtcaatgaag gcacttttcg cgctgacctg ctcgaccgac tggcttttga
    26641 tgttgtacaa ctgccaccac tgcgcgagcg cgaaagcgac ataatgttga tggcagaaca
    26701 ctttgccatc cagatgtgtc gggaaatcaa gctgcctctg ttcccggggt ttacggagcg
    26761 cgccagagaa acattgctga attatcgttg gccgggaaat attcgtgaat tgaaaaacgt
    26821 ggtggaacgt tcagtgtatc gccacggcac cagcgattat ccgcttgatg acatcattat
    26881 tgatcccttt aaacggcgtc cgcctgaaga cgctatcgcc gtttcagaaa ccacctcgct
    26941 tccaacactg ccgctggatt tacgtgagtt tcagatgcag caggaaaaag agttgctgca
    27001 actcagtttg caacaaggga aatataacca gaagcgcgcg gctgaattac tggggttaac
    27061 ttatcatcag ttccgcgcgt tgttgaaaaa gcaccagatt tagcgaagtt gagcaataca
    27121 acttgccgga tgcggcgtga atgctttatt cggcatacat aatcaatacg tcgtttgcag
    27181 gcctgataag cgcagcgcat cagacattat tgcgttgggc tattaatcgc tcagcgcatc
    27241 ctgcaaaatt ttcattgccg catcgaattg cgcatccggg atggtcagcg gatacaggaa
    27301 gcgaatcacg ttgccgtatg cgccacaggt cagcaggagc agcccctgcg ccagcgcgcg
    27361 ttgctggatt ttctgtgcaa tcgccgctga cggctcgccc gtttgcggat cgttaaactc
    27421 tgccgcaatc atagacccca gaccgcgtac cgccgcgatg gccggaacgc tttctttggc
    27481 atcaatcagc gtgtttgtca gacgctggcc cagttgattc gcgcgttcgc agagtgattc
    27541 tttgtcgata atgttgagca ccgcgtgcgc ggcagccacc gccagcgggt taccggcgta
    27601 ggtgccgcca agcccgcccg gcgcgggtgc gtccataata ttcgcgttac cgaccacgcc
    27661 cgaaagcggc atcccgcccg cgaggctttt cgccatcgtc attaaatccg gcttatcggc
    27721 gtaatgatcc atggcaaaca gcttaccggt acgcgcaaag ccgctttgca cttcatcagc
    27781 aatcatcaca ataccgtgct cgtcgcacag gcggcgaata gcggcaacca gctcttttgg
    27841 cgcaacgttg aaaccgccct cgccctgcac cggttcgaaa ataatcgccg ccacctgctt
    27901 cgcttcgatg tctgatttaa acaagcgttc gatggcgtcg agggagtcct gtgttgaaat
    27961 gccgtgtaaa tctgacggat aaggtacgtg atacaccgaa ccagggaacg ggccgaagcc
    28021 gattttgtac ggcgcaactt ttccggtcag cgccatggtc atatacgtac gaccgtgaaa
    28081 gccgccgcta aacgcaatca cgccagggcg tccggtatgg gcgcgagcaa ttttcaccgc
    28141 gttttccacc gcttccgcac cggtggtgaa gaacgcggtt ttggcctgcc cgctcaccgg
    28201 ggcaagggcg ttgattttct ccgccagggt gacgtagctt tcatacggca caatctgata
    28261 cgcggtgtgg gtaaactgtt gcagttgctg ctccaccgcc gcgaccagat cagggtggcg
    28321 atgtccggta ttcagcaccg caatgcctgc ggcgaaatcg atgtactcgt tgccctcaac
    28381 atccttcagc gtggcgtttt cagccgactg ggcgaagaag ttacacatca cgccaacccc
    28441 gcgcggagtg gcagaaagac gacgctgatg gaattcattg ttgctcatgt ttcacacctt
    28501 ttgactcttc acggttaatc ggaaattaaa cgcccagacg atcgcgcagg ctgtaatacg
    28561 ccgcgcccat cgcggtaaac ggcacacgca gcgtgcgccc gccggggaac gggtaatgcg
    28621 gcagattggc gaaggcatcg aaacgttcgg cgtcgccgcg cagcagttcg gcaatcaaac
    28681 gtccggctag atgagtacag gtcacgccgt ggccgctgta gccctgcatg taatagatgt
    28741 tggtatcgag gcgaccaaac tgcggcatac gcgacagggt cagcaggaag ttgcccgtcc
    28801 agcggtaatc aattttcacg cccttcagct gcgggaaggt tttcagcagt ttcggcacca
    28861 caaggcgctc aacgtcatcc gggtcgcgcg cgccgtagac cacgccgccg ccgtaaagca
    28921 ggcggttgtc ggcggtaaga cggtagtaat caagcagata gttacagtct tccacacagt
    28981 agtttttcgg gatcagcgaa cgggctaaat cttccgacag ccgttcggtg gtgatcacct
    29041 gcgtgccgca cggcatgctg cgtttcgcca gttccggctc tactttatcg cccagatacg
    29101 cattcccggc gacaatcaca tacttcgccg tcacctgacc tttggcagtt cgcacaacgg
    29161 ctggcgtggt gtgctggatc tgcgtcacgg cagaaagttc atacacgcgc ccgccgttga
    29221 ggcggatggc gtccgcttcg ccaatggcaa ggtttagcgg atgaatatgc ccaccgctgt
    29281 gatccagcag cgcaccggta tagcgatcgc tggctacttc acggcgaatg gcgttggcgt
    29341 ccagcaattc cagctgttta ttgccgtagc gttcccagtt ctctttctgc tcttcaagtg
    29401 tggcgagctg tttatcattc atcgccacaa acaggccgcc ggggcggtag tcgcagtcaa
    29461 tctgataacg tttgatacgt tcgcggatga tctcaccacc ttcgaacatc atgctgccga
    29521 gcatacgggc ggtgtccatg ccgtagcttt tttcgatcac gtcgatgtcg cggctgtagg
    29581 agttcacaag ctgtccgcca ttgcgcccgc ttgcgccaaa gccgatgcgt gaggcttcga
    29641 gaaccactac gtcaaagccc gcttccgcca gatgcagcgc ggaggagagc ccggtatagc
    29701 cgccgccaac cacgcaaacg tcgcaggtga ttgactcatt cagcgtgtcg aatggcgcgt
    29761 atttattcgc actggcggcg taataactgc tggtatgttc ggtcatttca ggcctccagg
    29821 cttatccaga tggttttcag ttcggtgaat ttttcaaggg catgcaggga tttgtcgcga
    29881 ccgttgccgc tctgcttata gccgccaaac ggcacggtca tatcgccgtc gttgtagtta
    29941 ttgacgaaga cggaaccggc tttcaggcgg cggctcatac ggtgcgcgcg ggagaggtcg
    30001 cgcgtccata ccgccgcgcc aaggccgtac tggctgtcgt tggcaagctg tagcgcctgt
    30061 tcttctgatg tgaaacgcgt gaccaccagc accggaccga aaatctcttc gcggcttaag
    30121 gacgcattcg ggtctacatc cacaaagatg gtcgggccga tggcggcagc cagctcagcg
    30181 ttacgaccat ccagcagcag ttgccctttg ctttcgcctt cccgaataaa gctatggacc
    30241 gagtcggcgt gggcgcagtc gattaaggtg cccatggtgg ttgcgggatc aagtggatgg
    30301 cccggctgcc agttttgcgc ctgctgtttt aacagggcta agaattcatc ggcgatgctc
    30361 tcttccagca acaaacgcgt tccggcgatg cacacctggc cctggttgta gaagatgcct
    30421 gcggcggtgg cgcttgccgc tttttgcaga tccggacaat cggcgaaaac gaggttggcg
    30481 cttttgccgc ccgcttccag ccagacgcgt ttcatgttgc tgtcgcccgc atctttcagc
    30541 agctgtttcc cggtacgggt tgaaccggta aaggcaatgg cgtcgatatc gttatgacgc
    30601 gacagcgcct gccccgcttc atgaccaaaa cccgtcacca cgttcaacac accatccggc
    30661 aagcctgctt ctttcgccag ccccgcgaga cgaatcgcac tgagcggtga tttttcagac
    30721 ggttttagaa tcacgctgtt tcccgccgcc agcgccgggc cgagtttcca gcaagtcagc
    30781 aacagcggga agttccacgg cacgatggcg gcaatcacgc cgaccggttc acgcacgatc
    30841 atcgccagct catggctact ggtggtcgcc acttcgccat acactttgtc gatcgcttcg
    30901 gcgtaccagc gaatggcgcg cgccgcgccg ggaatatcat cacgcagact gtgacgaatc
    30961 ggtttgccgg tgtcgagagt ttccagcagt gccagctctt cggcgtgggc ttccattaaa
    31021 tcggcgagtt tattcagtac cgctttacgt ttagccggag aagagagtga ccagtcgccg
    31081 cgttcaaata cgccgcgtgc tgcgctcacc gcacggtcga tatcgacgct cttgccgcgg
    31141 gcaattttcg ccagcggtgc ctgggtgacc ggatcaacgg tttcaaaggt ttcattttcc
    31201 gccgcagcag tatattcacc gttaataaat aagcggtttt caatggcgag acttaacgct
    31261 ttatcctgcc agtaagccag atgatgaaaa ttcattatga ctcctgtttc acgtctatca
    31321 gatatatgca gtttgtgaac gtcagggcgc agagatcccc gggccgtatg cccgtttatt
    31381 tctggcgaca ttctttatat acacttcatc cttcaagccg cctctgcctt ggctgcgttc
    31441 gctcccccga atcacttacc tgagtaagct catcgggatt cgctcacttg ccgccttgat
    31501 gcaacttgaa tgattttgtg tatagattgc cgggcgcatt gcgcttaccc ggcttacaga
    31561 acaaaaagat taaaacgtgg tgggcgtatg ggcgctgata attcggcaaa tacctgccga
    31621 cgtattactg aaactgtgcg ggatgccggt attaatggca taactttgcc ccgcgacgag
    31681 gtggtaatcc tgaccattaa tcgtcagaac aatttcacct tccagtactg tgcctatttc
    31741 ctcaccctga tgcttaattc tttccccagt ggttgtgccc ggctggtacg tttcaaagat
    31801 catcgccagc gtgcgattcg ggttaccgtt atgaaccagc ttcattgaca caccctgact
    31861 gcccatctca attaagtcgt cctgattaat gacgacctgc ggctcatcag gtttttccgg
    31921 ctcggaaaag aattccgaga gtgacagacc atacaccttc agcagctttt gcagcgtact
    31981 gatggcaggg ctgactttat cttgttctat cgtactgata gcactgtgag tcagcccgga
    32041 gagttcggcg gcacgacgtt gtgaaagccc ctgctgctgg cggatttccg acaagcgttt
    32101 tcctggcgcc agtccctcat cactcatatt tgcatttcct taaactgtag tggtcagagt
    32161 cgctgttttt cagcgatatg gtgctgacaa gcggtgataa agccctcgaa caatatacgc
    32221 gaaagcgcgt actcgctact gttccattcc gggtgccact gtacgcccag cgcaaaagga
    32281 tgattgatga cgctaaccgc ctcaactaag ccatccggcg agcgagcttc aacacgcaac
    32341 cgtgggctaa cgaccttcgc cccttgtcca tgcagagagt ttacccaaaa gttgctacat
    32401 tcaggtaaca acgcagacag taatcccccc tcttcaacct gaacttcatg cgacggtgca
    32461 tattgctgtt ccaccggcag ttcgggatct tcccgatgtt ccagcaattc aggctgttcg
    32521 cacagcttgc gatgcaacga accaccggtt gccaccacca gttcctgtaa accccggcag
    32581 atggcgaaaa tggggatgcg cctttcgagt gcggcattaa ttaacgccat gctcagaaga
    32641 tcacgcccgg gatcggcgtc aggctcatcg ccgttttcac catataggtg cggctgcaca
    32701 ttgctgggac taccaggaag ataaatgcca tcgagtttcg gcaaaagttg ttcaagtaat
    32761 gacggttccg ccagcgcatg tggtaacgca attggcaagc cgcctgcgtg gatgatggca
    32821 ttcaggtact tttcttgcag agtctgggtc gcatgaccct taagcctgtt cctgcacatt
    32881 acgacaccga taaccggatt gttcattata ttttccatgc tcaatctcac aaagtggact
    32941 aaattatcgc cattactgcg ctttaaatga cctttatgtt caatattttt tcaatctagc
    33001 agtgggtttt catttttgca aactcaattt aacatttgac aaacatttag tttgcataca
    33061 gattcgaatg gtggtcatta tattttacgc tttgataacg agcggaaaac aaaccaaagg
    33121 cgaagaatca tggaaaccaa tatcgttgaa gtagagaact ttgttcagca gtcagaagag
    33181 aggcggggta gcgcctttac gcaggaagtg aaacgctacc tggagcgcta cccgaatacg
    33241 caatatgttg atgttttact taccgattta aatggttgct tccgtggaaa gcgtattcct
    33301 gtttcaagcc tgaagaagct cgagaaaggg tgttatttcc cggcctcggt atttgcaatg
    33361 gatattctgg gcaacgtagt agaagaagca ggtctgggtc aggaaatggg cgagccggat
    33421 cgtacctgtg ttcctgttct cggttcctta actccttctg ccgccgatcc agagtttatc
    33481 ggtcagatgc tcctgaccat ggtcgatgaa gatggcgctc cctttgacgt tgagccgcgg
    33541 aacgttctta accgcctgtg gcagcagctg cgccagcgcg gattgttccc ggtcgtagcg
    33601 gtagagctgg agttctattt actggatcgc cagcgcgacg ctgaagggta tctgcaaccg
    33661 ccctgcgcgc ctggcaccga tgaccgcaat acacaaagcc aggtttactc cgttgataac
    33721 ctcaaccact tcgccgacgt gctcaatgat attgatgaac tggcgcagtt acagctgatt
    33781 ccggcagatg gcgcggtcgc tgaggcctcg ccgggtcagt ttgaaatcaa cctttaccat
    33841 actgataacg tgctggaagc ctgcgatgat gcgctggcac taaaacgcct ggtgcgtctg
    33901 atggcagaaa agcataagat gcacgccact tttatggcga agccgtatga agagcacgcg
    33961 ggcaacggaa tgcatatcca tatcagtatg caaaataatc gtggcgagaa cgtgctttct
    34021 gacgcggaag gcgaagattc gccgctgctg aaaaagatgc tcgccgggat gattgacctg
    34081 atgccgtcgt cgatggcgtt gctggcacca aacgtgaact cgtatcgccg cttccagccg
    34141 ggaatgtatg tgccgacgca ggcgtcgtgg ggccataaca accgcaccgt cgccctgcgt
    34201 attccgtgcg gcgaccgtca taatcaccgc gtggaatatc gcgtggcggg tgccgatgcc
    34261 aacccatatc tggtgatggc agcgattttt gccggtattt tgcatggcct tgataacgag
    34321 ctgccgttgc aggaagaagt cgaaggcaac gggctggaac aggaaggctt accctttccg
    34381 attcgccaga gcgatgccct gggtgagttt atcgagaatg atcacctgcg ccgctattta
    34441 ggcgaacgct tctgccatgt ctatcacgcc tgtaaaaatg atgagctgtt gcagtttgag
    34501 cgcctcatca cagaaacaga aattgagtgg atgttgaaaa acgcgtaata ccccttgcgg
    34561 ggttgtttca tcggcctgag cggagccagg cctgacattt gctggtaatc cagacatttc
    34621 ccggatgtcg cgttatggag cgcgggcggc aacgggcttc cgttaacgat taagactgtg
    34681 acgaggaaat gagatgatgc cgatgattaa atccccgcat ggcgagggag gctgcgtatg
    34741 cgcccctcct gctactgact ggactccgcc ccctttgctt cccttgctaa acaggtttga
    34801 tttcaggtcg acacgaccgc aaaccttatt acgcagggga ggcagcaatt atggctatta
    34861 attcaccact gaatattgct gcgcaacccg gcaaaacccg tctgcgaaaa tcactgaaat
    34921 tgtggcaggt ggtgatgatg ggtctggcct atctcacgcc gatgaccgta tttgatactt
    34981 ttggtattgt ctccggcatt agcgacggtc acgttccggc gtcctatttg ctggcgctgg
    35041 cgggtgtgct gtttaccgct atcagctacg gcaaactggt tcgccagttt ccggaggccg
    35101 gttcggccta tacctacgcg caaaagtcga ttaacccgca cgtcggattt atggtcggct
    35161 ggtcatcgct gctggattat ctctttttgc cgatgattaa cgtcttgttg gcgaaaatct
    35221 atctctccgc cctcttcccg gaagtgccgc cgtgggtgtg ggtggtaacc ttcgtcgcca
    35281 ttttaaccgc cgcgaatctg aagagcgtca acctggtcgc taacttcaat accctgtttg
    35341 tactggtgca aatctccatc atggtggtgt ttatcttcct ggtagttcag ggactgcata
    35401 aaggagaagg cgttggcacc gtctggtcac ttcagccgtt tatcagcgag aacgcgcacc
    35461 tgatcccgat tattaccggg gcgacgattg tctgtttctc cttcctcggt ttcgatgcgg
    35521 tgaccacgct ttcggaagag acgccggacg ccgcacgcgt gatcccgaaa gccatcttcc
    35581 tgacggcggt ctacggtggc gttatcttta tcgcggcgtc gttctttatg cagctgttct
    35641 ttcccgatat cagccgcttt aaagatccgg atgccgcact gcctgaaatt gcgctgtacg
    35701 tcggcggcaa gctcttccag tcgattttcc tctgcaccac gtttgtgaac acgttagcct
    35761 ctggcctggc ttcacatgcc agcgtgtcgc gtctgctgta tgtgatgggg cgtgacaatg
    35821 tgtttccgga gcgcgtgttt ggctatgtgc acccaaaatg gcggactccg gcactcaacg
    35881 tcattatggt cgggattgtc gcgttgtcgg cgctgttctt cgatttagtt accgccacag
    35941 cgttaattaa cttcggtgct ctggtggcgt ttaccttcgt gaatctgtcg gtgtttaatc
    36001 atttctggcg gcgtaaagga atgaataaaa gctggaagga tcacttccac tatttgctga
    36061 tgccgctggt tggcgcgctg acggtgggtg tgctgtgggt taacctcgag tcaacgtcac
    36121 tgacactcgg tctggtatgg gcttcgttag gcggcgcata tttatggtat ctgatccgcc
    36181 gctatcgcaa agtgccgttg tatgatggtg acagaacgcc ggtgagtgaa acgtaatagt
    36241 cggatgcgcg gcgcattatc ctgcctgagg gtaacgcgcc gcgccattaa ctataaaaag
    36301 atgtatgacc cggatcacga gtgctatcat ccgcccttat gcttagcgca ttaaccccag
    36361 gggcaaataa tgaaccacga tatcccgcta aaatattttg atattgccga tgagtacgcg
    36421 accgaatgcg cagagcctgt cgcagaggct gaacgcacac cgctggccca ctacttccag
    36481 ttactgctca cccgtttgat gaacaatgaa gagatcagcg aagaagccca acatgaaatg
    36541 gctgctgaag cggggattaa tcccgtacgc attgatgaga tcgcagagtt tttgaatcaa
    36601 tggggtaatg agtaatgatt taattgcaga cgatcggtca gtatcgttgt ccggataaag
    36661 cgttcacgcc gcatctggct aacaatgaca cgcactgcag cgtgctttaa tgggtttctc
    36721 acgatcgtac cgctctgctc ctcgctaagt tgttctgtga gttatagtta tagagaaatc
    36781 acgggtacga atgttgaaca gatggcgacg tcacctgttc aacgaccaac cacacggccc
    36841 tcattacgac ccctgaacac aaacggtaat ggcaaatgtc attaaatatt agtaagatag
    36901 cctctttaca taaaatccct taatattatg cgccaggtat tatcgtctct tttggtgatt
    36961 gctggacttg tgagtggtca ggcaatcgcc gcgcctgaat cccccccgca tgctgatatc
    37021 cgcgacagcg gttttgtcta ttgcgtcagc gggcaagtca acacctttaa cccatccaaa
    37081 gcgagcagtg ggttaattgt cgataccctt gccgcccagt tttatgatcg actgctggat
    37141 gtcgatccct atacctatcg cctgatgccg gaacttgccg aaagctggga agtactcgac
    37201 aacggcgcga cctatcgctt ccacctacgt cgcgatgttc cgtttcaaaa aaccgactgg
    37261 tttactccca ctcgtaaaat gaatgccgac gatgtggtgt ttaccttcca gcgaattttt
    37321 gaccgcaaca acccgtggca taacgtcaac ggcagcaact tcccctactt cgacagcctg
    37381 caatttgccg ataacgtgaa aagcgtccgt aaactggata atcataccgt tgagttccgt
    37441 ctggctcagc cggatgcttc ttttttgtgg cacctcgcaa cccattatgc ttcggtcatg
    37501 tcggcagaat atgcccggaa gttagagaaa gaagatcgcc aggagcaact cgaccgtcaa
    37561 ccggtcggca ctgggccata tcagttgtcg gaataccgcg ccgggcaatt tattcgccta
    37621 caacgtcatg atgacttctg gcgcggtaaa ccgttaatgc cgcaggtggt ggtggattta
    37681 ggctccggcg gcaccggacg tctgtcgaaa ctcctgaccg gggaatgcga cgttctggcc
    37741 tggcctgctg ccagccagct atccattttg cgtgacgacc cgcgcttgcg tttaacgctg
    37801 cgtcctggga tgaacgtcgc ctatctggca tttaacaccg ccaaaccgcc gctaaataat
    37861 cccgctgtcc gccatgcgct ggcactggcg attaataacc agcgcctgat gcaatccatc
    37921 tattatggta cggctgaaac ggcggcctct attttaccgc gcgcctcgtg ggcctatgac
    37981 aacgaggcta aaattactga atacaatccg gcgaaatcgc gcgaacagtt gaaggcgttg
    38041 gggctggaaa atttaacgct gaaactgtgg gtgcccacac gttcgcaggc gtggaacccc
    38101 agtccactga aaactgccga actgattcag gcggatatgg cgcaggttgg cgtaaaagtg
    38161 gtgattgtgc cggtagaagg tcgctttcag gaggcgcggt tgatggatat gagccatgat
    38221 ctgacgttat ccggttgggc gacggacagt aacgacccgg acagtttctt ccgtccgtta
    38281 ctgagctgcg cggcaattca ttcacagacc aacctcgccc actggtgcga tccgaaattc
    38341 gacagcgtgt tgcgtaaggc gctctcctcg cagcagctgg cagcgcgtat tgaagcctat
    38401 gacgaagcgc agagtattct ggcgcaggaa ttacctattc tgccgctggc gtcgtcattg
    38461 cgtttgcagg cctatcggta cgatatcaaa ggtctggtac ttagcccgtt tggtaacgcc
    38521 tcctttgctg gggtgtatcg cgagaaacag gatgaggtga aaaaaccatg attatcttca
    38581 ccttacgccg cattttgtta ttgattgtca ccttgttcct gctgaccttc gttggcttca
    38641 gtttgagcta tttcactcca cacgcgccgt tacaaggcgc gtcactgtgg aatgcctggg
    38701 tgttctggtt taacggcctg atccactggg attttggtgt ttccagcatt aacggtcaac
    38761 cgattgccga acagttaaaa gaggtcttcc ctgcgacgat ggaactgtgc atcctcgcct
    38821 tcggttttgc gctgattgtt ggcatccccg tggggatgat tgccgggatt acgcgccata
    38881 agtggcagga taacttgatc aacgccatcg ccttgctggg cttttcaatc ccggtgttct
    38941 ggctggcgct tctgttgacg ctgttttgtt cactcacgct aggttggttg cccgtttcag
    39001 ggcgtttcga tctgctgtac gaagtgaaac cgattaccgg gtttgcgttg attgatgcct
    39061 ggctttcgga ctcaccatgg cgggatgaaa tgatcatgag cgcaatccgc catatgatat
    39121 tgcccgtgat caccctgtcg gttgcgccaa caactgaagt gatccgcctg atgcgtatca
    39181 gcactatcga agtgtatgac cagaactatg taaaagcggc ggcgacccgc ggtttgtcgc
    39241 gctttactat tttgcgtcgc cacgttctgc ataacgcgtt accgccggtt attcctcgcc
    39301 tgggcttaca gttttccacc atgctgacgc tggcgatgat caccgaaatg gtttttagct
    39361 ggccgggttt agggcgctgg ttaattaacg ccatccgcca gcaggattac gcagccattt
    39421 ccgccggagt gatggtgtgt ggctcactgg tcattattgt taacgtgatt tctgatattt
    39481 tgggtgccat ggctaaccct ctgaaacata aggaatggta tgccttacga tagcgtatac
    39541 agcgaaaagc gcccgccggg cacgctgcgt accgcctggc gcaaatttta tagtgacgcc
    39601 tctgcgatgg tcggcctgta cggctgcgcg gggctggctg tactgtgtat ttttggcggc
    39661 tggtttgcgc cttacggcat cgaccagcaa tttctcggtt atcaattatt gccgccgtca
    39721 tggtcgcgct atggcgaagt ttctttcttc ctggggactg acgacctcgg acgcgatgtt
    39781 ttaagccgtt tactgagcgg agctgcgcca accgtgggtg gcgcatttgt ggtgacgctt
    39841 gccgcgacga tctgtggcct ggtactgggc accttcgccg gggcgacaca cggcctccgc
    39901 tcagcggtgc ttaaccatat tctggatacc ttactggcga tcccttcgct gctgttggca
    39961 attatcgttg tagcgtttgc cggaccgagt ttgtcccacg ctatgtttgc tgtctggctg
    40021 gcattgctgc cgcgtatggt acgttcgatt tacagcatgg tgcatgacga actggaaaaa
    40081 gagtacgtta tcgccgcccg tctggatggc gcatcaacgc tgaatattct ctggtttgct
    40141 gtgatgccaa acatcaccgc cgggctggtc accgagatca cccgcgcact gtcgatggca
    40201 attctcgata tcgccgcgct gggctttctc gatctcggcg cacaactccc ctcgcctgaa
    40261 tggggagcga tgctcggtga tgcgctggaa ctgatttatg tcgccccgtg gactgtcatg
    40321 ctgccaggtg cggcaattat gattagcgtg ttgttggtta acctccttgg cgatggcgtt
    40381 cgtcgtgcga ttattgcggg ggtggaataa tgccattact cgatattcgt aacctgacca
    40441 ttgaatttaa aaccggtgat gagtgggtta aagccgtcga ccgggtaagt atgaccttaa
    40501 ccgaaggtga aatccgcggt cttgttggtg aatccggttc cggcaagagt ttgattgcga
    40561 aagcgatttg tggggtgaat aaagataact ggcgtgttac tgctgaccgt atgcgttttg
    40621 atgatatcga tttgctgcgt ctctccgcac gcgaacggcg caaactggtt ggccataacg
    40681 tgtcgatgat tttccaggaa ccgcagtcgt gtcttgaccc ttcagaacgt gtgggtcgcc
    40741 agttgatgca aaacatccca gcctggacct ataaaggccg ttggtggcag cgttttggct
    40801 ggcgcaaacg ccgtgccatt gaactgctgc accgcgtggg gattaaagat cacaaagatg
    40861 cgatgcgcag ttttccctat gagttgaccg aaggtgaatg tcagaaagtg atgatagcca
    40921 ttgcactggc gaatcaaccg cgtctgctga ttgctgacga accgaccaac tcaatggagc
    40981 caacaaccca ggcgcaaatc tttcgcctgc tgacgcgtct caaccaaaac agcaatacca
    41041 ctattttgct tatcagccat gacttacaaa tgcttagcca atgggcggat aaaattaacg
    41101 tgctttactg cggtcaaaca gtggaaaccg cgccgagtaa ggagttggtg acgatgccac
    41161 atcatcctta tacccaggcg ctgatccgcg cgataccaga cttcggcagc gcgatgccgc
    41221 ataaaagtcg cctcaatacg ctgcccggcg ctatcccact gctggaacag ttaccgattg
    41281 gttgtcgtct ggggccacgt tgcccgtatg cacaacgaga atgcattgtg acgccacgtt
    41341 tgacgggggc gaaaaatcat ctctatgcct gtcatttccc gctgaacatg gagaaagagt
    41401 gagatgatcg aaacgctgct tgaagtgcgt aatttaagta aaacgttccg ctaccggacc
    41461 ggctggtttc gtcgtcagac cgtagaagcg gtaaaaccct tgagctttac gctacgtgaa
    41521 ggccagacac tggcgattat tggcgagaat ggttcgggta aatccacgct ggcaaaaatg
    41581 ctggcgggaa tgatagagcc taccagcggc gagttattga ttgacgatca tccactgcat
    41641 tttggcgatt attccttccg cagtcagcgt attcgcatga tttttcagga tccttcgacc
    41701 tcgttgaatc cccgtcaacg tatctcgcaa attctggatt ttccactgcg cctgaacacc
    41761 gacctggaac cggaacagcg tcgcaaacag attattgaaa cgatgcgtat ggttgggctg
    41821 ctaccggatc acgtcagtta ctatccgcat atgttggcac ccggacaaaa acagcgtctg
    41881 ggtctggcgc gcgcgctgat attgcgccca aaagtcatta ttgccgatga agccctcgct
    41941 tcactggata tgtcgatgcg ttcgcagttg attaatctga tgctggagtt acaggaaaaa
    42001 cagggcattt cgtatattta tgttacccag catatcggaa tgatgaagca cattagcgac
    42061 caggtgctgg tgatgcatca gggcgaggtt gtcgagcgag gcagcaccgc ggatgtgctg
    42121 gcatcaccgc tgcatgaact caccaaacgg ctgattgccg ggcactttgg cgaagcattg
    42181 acagcggatg cgtggcgtaa agatcgctaa ttttctgaat cctccttcca gattattcac
    42241 ctcattctga caagagatta aatcttgtca gaatgaggtg atggataaaa taaaatcaaa
    42301 tgcgcgtgat ttaagaagca atctcacttt gcaggaacga aagctctggc gatatcttcg
    42361 cagccgacgt tttggtgatt tcaaatttcg ccgtcaacat ccggtgggga gctacattct
    42421 cgattttgct tgctgctcgg cgcgtgtagt cgttgagctg gatggtgggc agcatgattt
    42481 ggcagttgcc tatgattcca ggcgcactag ctggcttgag tcgcagggct ggaccgtgct
    42541 gcgtttctgg aataacgaga ttgattgtaa tgaggagacg gtgctggaga atattctgca
    42601 ggaactgaac cgccggtcac cctctccctg aaagagcgag ggggccgacc gagccgaata
    42661 gctgttgtgg tgaaaatatg gtgacagttc tggagaatat tcggcaaggt ctgaaccgtc
    42721 ccagccatcg ccctgaaagg gttagggggc tgtataagcc tgtttgttgc tggggtgaca
    42781 atatttgcac aatacggtcc cctcgcccct ctggggagag gattagggtg tggggaaaag
    42841 cgcccaccct gcccgcaccc tgctccggtc ggacctggca gctatagcca ctcacagcca
    42901 ggttgattat aataaccgtt tatctgttcg tactgtttac taaaacgacg aatcgcctga
    42961 ttttcaggca caacaagcat caacaataag gattaaagct atgggttttc tttccggtaa
    43021 gcgcattctg gtaaccggtg ttgccagcaa actgtccatc gcctacggta tcgctcaggc
    43081 gatgcaccgc gaaggagctg aactggcatt cacctaccag aacgacaaac tgaaaggccg
    43141 cgtagaagaa tttgccgctc aattgggttc tgacatcgtt ctgcagtgcg atgttgccga
    43201 agatgccagc atcgacacca tgttcgctga actggggaaa gtttggccga aatttgacgg
    43261 tttcgtacac tctattggtt ttgcacctgg cgatcagctg gatggtgact atgttaacgc
    43321 cgttactcgt gaaggcttca aaattgccca cgacatcagc tcctacagct tcgttgcaat
    43381 ggcaaaagct tgccgctcca tgctgaatcc gggttctgcc ctgctgaccc tttcctacct
    43441 tggcgctgag cgcgctatcc cgaactacaa cgttatgggt ctggcaaaag cgtctctgga
    43501 agcgaacgtg cgctatatgg cgaacgcgat gggtccggaa ggtgtgcgtg ttaacgccat
    43561 ctctgctggt ccgatccgta ctctggcggc ctccggtatc aaagacttcc gcaaaatgct
    43621 ggctcattgc gaagccgtta ccccgattcg ccgtaccgtt actattgaag atgtgggtaa
    43681 ctctgcggca ttcctgtgct ccgatctctc tgccggtatc tccggtgaag tggtccacgt
    43741 tgacggcggt ttcagcattg ctgcaatgaa cgaactcgaa ctgaaataat cgttctgttg
    43801 gtaaagatgg gcggcgttct gccgcccgtt atctctgtta tacctttctg atatttgtta
    43861 tcgccgatcc gtctttctcc ccttcccgcc ttgcgtcagg ataacgattt cctttacgac
    43921 caaggagcgc ccatggaaca acgccacatc accggcaaaa gccactggta tcatgaaacg
    43981 caatccagta ctacggagta tgacgttctg cctctggtcc cggaagccgc aaaggtcagc
    44041 gatccctttc tactcgacgt gatccttgaa aaagaaacgc tggccccctt cctttcatgg
    44101 ctggaccctg cgcgtgttct tgcagtggat ttgttccctg accagcttac cgtgacccgt
    44161 tcacagacct tcaccgctta tgaacgcttg tcgacggccc tgacggttgc tcaggtttgc
    44221 ggcgtccagc ggttatgtaa ctactattcg gcgcgactta cgccgctccc cgggcctgat
    44281 tccaccaggg aaagtaatca tcggttggca caaatcacgc aatatgcccg ccaactggct
    44341 agctcgcctt ctattatcga caaccgatcg cgccagcatc tgaatgacgt cggtcttact
    44401 gcctgggact gtgtgatcat tagccaaatc attggtttta ttggctttca ggcgcggaca
    44461 attgcgacat ttcaggctta tctcgggcat ccggtacgct ggttacccgg gctggagata
    44521 caaaactacg ccgacgcgtc actgtttgct gatgaatcat tacgctggcg aagcagctat
    44581 gaggtggaaa aactacctga agagcacaca aaaagttcaa ctgcagaact ttgccaactg
    44641 gccgaaatac tctctctcca ccctatttca ctttcccttc tcgaaaagtt gttaaacagc
    44701 acacggggca atacacagcc ggataatcag cttgcggcgt tgttatgcgc gcgtataaat
    44761 ggcagtcctg cttgttttgc cacctgtatg gattcatcaa atgaatataa aaaaatcagc
    44821 acccttatgc gcaagggcga aaatgaaatt aaccaatggg ctgaccgtca ttctgttgag
    44881 cgcgctaccg ttcaggcgat acaatggctg acccgagcac ccgatcgctt tagcgccgcc
    44941 cagttcagcc ctttactcga acacgaaaaa tcatcaacgc agattattaa tctgctggta
    45001 tggagcgggc tgtgtggctg gataaatcgc ttaaaaatcg cgttgggtga gacatattaa
    45061 ccttgccgcg tcagacagat tcgcgtaaaa ctgtcagccg ctctaatggc caccaaaata
    45121 gacaattatg tttcaggaca acccgctgct agcgcagctt aaacagcaac tgcattccca
    45181 gacgccacgc gctgaagggg tggtaaaagc cacagaaaaa ggctttggct tcctggaagt
    45241 cgacgcgcaa aaaagttatt tcattccgcc gccgcagatg aaaaaagtca tgcatggcga
    45301 ccgaattatc gcggtgatcc acagtgaaaa agaacgtgaa tccgcagagc cagaagaact
    45361 ggttgaaccg ttcctgactc gtttcgtggg taaggttcag ggcaaaaatg accgtctggc
    45421 catcgttcct gatcatccac tcttaaaaga cgccattcct tgccgcgcag cccgtggcct
    45481 gaaccacgag tttaaagaag gcgactgggc ggttgccgaa atgcgccgtc atccgctgaa
    45541 aggcgatcgt tctttctatg cagaactgac acaatacatc acttttggtg acgatcactt
    45601 tgtaccgtgg tgggttaccc ttgcacgcca taatctggaa aaagaagcac cagacggcgt
    45661 cgctaccgaa atgctcgatg aaggtctggt tcgtgaagat ctgaccgcgc tggattttgt
    45721 caccatcgac agtgccagca cagaagatat ggatgacgcc cttttcgcta aggcgttgcc
    45781 ggatgacaaa cttcagctga ttgtggcgat tgccgatcca accgcgtgga ttgctgaagg
    45841 cagcaagctg gacaaagccg cgaaaattcg cgcattcacc aactatctgc ctggcttcaa
    45901 catccctatg ctgcctcgcg agctttctga cgatctctgc tcactgcgcg ccaatgaagt
    45961 ccgcccggta ctggcatgcc gcatgacgct ctccgctgat ggcaccattg aagataatat
    46021 cgaattcttt gccgccacca tcgaatccaa agcaaagctg gtgtatgacc aggtttctga
    46081 ctggctggaa aataccggtg actggcagcc tgaaagtgaa gcaattgccg aacaagtccg
    46141 tttgctagcg caaatttgcc aacgccgcgg cgagtggcgt cataaccacg cactggtgtt
    46201 taaagatcgc ccggattacc gctttattct cggtgaaaaa ggtgaagtgc tggatatcgt
    46261 cgccgagcct cgtcgcattg ccaaccgtat cgtcgaagaa gcgatgattg ccgctaacat
    46321 ttgtgcggcc cgcgtactgc gcgataagct cggttttggc atctataacg tgcatatggg
    46381 ctttgatccg gcgaatgccg acgcgctggc agcgttgctg aaaacgcacg gtctgcatgt
    46441 cgatgccgaa gaagtgctca cgctggacgg tttctgcaaa ctgcgtcgtg aactggatgc
    46501 gcaaccaact ggtttcctcg acagccgcat tcgtcgcttc cagtcatttg ctgaaattag
    46561 cactgaaccc ggtcctcact ttggcctcgg tctggaagca tacgccacct ggacatcgcc
    46621 gatccgtaaa tatggcgaca tgatcaacca ccgtctgctg aaagcggtta tcaaaggcga
    46681 aactgcgacg cgtccacagg atgagatcac tgtccaaatg gccgagcgtc gccgtctcaa
    46741 tcggatggca gaacgtgatg ttggtgactg gttatacgca cgcttcctga aagacaaagc
    46801 cgggaccgac acccgtttcg cggcggaaat tgtcgatatc agccgtggcg gcatgcgtgt
    46861 tcgtttggtt gataacggcg ctatcgcctt tattcctgca cctttcttac acgctgtgcg
    46921 cgatgaaatg gtttgcagcc aggaaaacgg caccgtacaa attaaaggtg aaacggttta
    46981 taaagtaact gacgttattg acgtcaccat tgccgaagtc cgcatggaaa cccgcagcat
    47041 tattgcgcgc ccggtcgcgt aatctccttt cacggcccat tccttatgaa tgggccgttt
    47101 atttccccgc tctaccttca tcatatcccg gcagtttttt aactgacttt cgtttgaaaa
    47161 ctggcgtttt tccaaaactg gattacaaat aaatatataa aaaaaacaat gcgtttagcc
    47221 gccgttaatt tcatgtaagt catgttgttc cttttattta accgcggact ccgctgttaa
    47281 ccggaggata tgcatcatga aaaccgttag ggagtccaca acgttgtaca actttctcgg
    47341 atcgcacaat tcatactggc ggttgacgga aagcagcgat gttttgcgct tttctaccac
    47401 cgaaaccaca gaacctgaac gtatgttgca gttatctgcc gaacaggctg ctcgaatcag
    47461 ggaaatgacg gtcatcacct ccagcctgat gatgagtctg accgtcgatg aaagcgatct
    47521 ttctgtgcat ctggtaggac gaaaaatcaa taaacgggaa tgggctggca acgcgtctgc
    47581 atggcatgac acaccggcgg ttgctcgtga tttatcacac gggctttcct ttgctgagca
    47641 ggtagtttct gaagcacatt ccgcaatatt cattctcgac agccggggga atattcaacg
    47701 cttcaatcgc ttatgtgaag attacacagg gttgaaagaa cacgacgtca ttgggcaaag
    47761 cgtgtttaaa ctgtttatga gccgtcgtga agctgcggca tccaggcgca ataaccgtgt
    47821 attttttcga agcggcaatg catatgaagt cgaactgtgg ataccaacac gtaaaggcca
    47881 gcggctgttt ctgtttcgca ataaatttgt ccacagcggc agtggcaaaa acgagatttt
    47941 tttaatctgt tccggtaccg acattaccga agagcgccgc gctcaggagc gactgcgtat
    48001 tctggcaaat accgacagta tcaccggact gccgaatcgt aacgcaatgc aggagttaat
    48061 cgatcacgct attaatcatg cagataacaa taaagttggg gttgtgtatc ttgatttgga
    48121 taattttaaa aaggtcaacg acgcctatgg gcatttgttt ggtgaccagt tattacgcga
    48181 cgtgtcattg gctattttaa gctgtctcga acatgaccag gtgttggcgc gtccaggtgg
    48241 cgatgagttt ctggtactgg catccaatac ctcacaaagc gcgctggaag caatggcatc
    48301 acgaattttg acccgcttac gcctcccctt tcgtattggt ttaattgaag tttataccag
    48361 ctgttcagta ggtattgcac tctctcccga acatggttcg gacagcgcgg ctattattcg
    48421 tcacgccgac acagcaatgt acacagcgaa ggaaggcgga cgaggacaat tttgcgtttt
    48481 tactccagaa atgaatcagc gggtatttga atatctctgg ctggatacca acttgcgtaa
    48541 agcactggaa aacgatcagt tggttattca ctatcaaccg aaaatcacct ggcgtggcga
    48601 agtgcgcagt ctggaagcac tagtacgttg gcagtcgcct gaacgtgggt tgattccacc
    48661 gttggatttc atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt
    48721 gattctcgat gtcgtacgcc aggtggcgaa gtggcgggat aaaggcatta acctgcgagt
    48781 ggcggtaaac atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    48841 ggttctccag gaactgaatt ttgaatactg cccaatagat gttgaactga cagagagttg
    48901 tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc
    48961 gcaagtgcat ctggacgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    49021 tccgatcgat gccatcaaac ttgaccaggg ttttgttcga gatattcaca aacagcctgt
    49081 ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    49141 cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    49201 gcggcaagga tttttgtttg ctaaaccgat gcccgccgtc gccttcgaac gttggtataa
    49261 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgttat attccgtcat
    49321 tatatatcgt attccaggat gataattagc cgttgctgtt aaacacttct cttttagcgg
    49381 caatttttgc attattcatt ttccactttt gctataagga tgtccttgat gtctgaattt
    49441 gatgcccagc gcgttgccga acgtattgat attgtgttag atatccttgt ggcaggcgat
    49501 taccactctg cgatccataa tctcgagata cttaaagcag aactcttgcg ccaggttgct
    49561 gaatctacgc cagacatccc gaaggcaccg tgggagattt aacaatcgtg ccttcaggtt
    49621 ttcctatttg aggatgcccg gcgcgtcagt atttttttat taagtattat aacgttataa
    49681 gaattacagc gatgaattcc cgacaacaaa ctattctaca gatggtcatt gaccagggtc
    49741 aggttagcgt aaccgatctg gcaaaagcca ctggagtttc tgaagttacc attcgccagg
    49801 atctcaacac actcgaaaaa ctgagttacc tccgccgtgc acatggcttt gctgtttcgc
    49861 ttgatagtga tgacgtcgaa acccgtatga tgagcaacta tacgctgaag cgtgaactcg
    49921 ccgagtttgc cgcgtcgctg gttcaaccgg gcgaaaccat ctttatcgaa aatggcagca
    49981 gcaatgccct actcgcccgg actctgggcg agcagaagaa aaatgtcact atcatcacgg
    50041 tcagcagcta catcgcgcat ttgctgaaag acgcgccttg tgaagttatt ttgctcggtg
    50101 gcgtgtatca gaaaaaaagc gaaagtatgg ttggcccttt gacacgccag tgcatccaac
    50161 aggtgcattt cagcaaagca tttattggta ttgatggctg gcaacctgaa actggattta
    50221 ccggtcgcga tatgatgcgt accgatgtgg tcaatgccgt gctggaaaaa gagtgcgaag
    50281 cgatagtcct gactgacagc tcgaaatttg gtgctgtaca ttcatactcc atcggtcccg
    50341 ttgagcgatt caatcgcgtg attaccgatt cgaaaatacg cgccagcgat ctgatgcatc
    50401 ttgagcacag taaactcacc gttcacgtcg ttgacattta accatttccc ttctctacgg
    50461 atgatttgca gtttggcaaa tcatccgctc taagatgatt cctggttgat aattaagact
    50521 atttacctgt tattaacact ctcaagatat aaaattatta tcagcgatat aacaggaagt
    50581 cattatcacc tgcgtgatat aaccctgcgc gcgagcagat ttcacggaat aatttcacca
    50641 ggcttattct tagctattat agttatagag agcttacttc cgtgaatcat aaattcagga
    50701 gagagtatta tgtttgtaac gagcaaaaaa atgaccgcgg ctgttctggc aattactttg
    50761 gcaatgtctc tgagtgcctg ttctaactgg tctaaacggg accgcaacac cgcaatcggc
    50821 gcgggtgcag gggcattagg cggtgcagta ctgaccgatg gcagtacgtt gggtacatta
    50881 ggtggtgcag ctgtcggtgg tgttattggt caccaggtcg gtaaataatc gtaaataaat
    50941 gtcgattatc gactcgcaat tataatacgc ttgattctgt tcagtacgaa ataactgaat
    51001 aatacgcaca gtaaaatatt cgaaagccac ggatatatcc gtggcttttt atgttaaccg
    51061 cctgcgagtt ttaccttcat ccctttcgct tcaagtagtg attttaataa atcacgctta
    51121 tcaccctgga tttcaataac tccatcttta actgctccgc cgcagccgca ttttttcttc
    51181 agttccgctg caagttttgt cagttcggca tcatcgagat cgacaccggt aatcaggcaa
    51241 acgcccttac ctttacatcc actggtctga cgctgaatac gcaccacacc gtcgccttta
    51301 gggcgtacag gggccgcttt gggctcatca atacgtccgg tttccgttga gtagaccaga
    51361 cggctgttgg aatcactcat catgcactcc gctgtaaaga ggcgttgatc gctttcagcg
    51421 tctgcgctgg atctaccgat tgcgttaccg ggcgaccaat caccatataa tcaacaccag
    51481 ccgacaacgc ctgttctggc gtcataatgc ggcgctggtc accagcttca ctcccctgcg
    51541 gacgaatgcc cggcgtaacc agtttgaact cctgaccgaa tacctgttta aagcgcacag
    51601 cttcctgagc agaacacacc acaccatcaa ggccacattt ttgcgtcagt gccgccagac
    51661 gttctgcata atctgcaggt gacagtgtca tgccaagatc gaccaggtcg ctggcttcca
    51721 tgctggtcaa cactgtcaca gcaatcaaaa gcggtgcatc tttgccaaac ggaaccagtg
    51781 cctcacgcgc tgcggtcatc atacgcgccc caccagaggc atgaacattc accatccaca
    51841 cgcctaagtc agctgcagca gcgacagcgt gcgctgcagt gttggggata tcgtggaatt
    51901 tcaggtcaag aaagatatca aaaccacgct gttgaagttc gcgcacaaac tgtggcccaa
    51961 acaatgtaaa catctctttg ccgaccttca gacgacaatc gcgtgggtcg atcttgtcga
    52021 caaaggccag cgcgtcatca cgattatgat aatcaagggc aacaaccaca ggagaattcg
    52081 taacagcgcg ggaagaagat gaagcagtta acgtcatgac cagaccttct tgatgatggg
    52141 caccggaagt ggcgcgaaac aggtaaacgg cgagcattct acccgtggaa ataaaaaatt
    52201 aacagttgcg atttctcccc tggcaggctt tttctgcgcc gtcgttgccg gagcacaatg
    52261 gaacattaat aattagtatg ttgtaactaa agtcggattt tttttaaaaa ttacaggcca
    52321 tcaagaccgc gaatcggttt aatggttgac caggcccgac aagacggaca atgccagtag
    52381 agggtgtatg cggtaaaacc acatttctgg caacgataac gaggcttact acgtaccttc
    52441 tcgccaacca tgtcacgcag caccatcagg ctctctttgg cacgcccttc ttccgcttca
    52501 tttaagtggt aatccattaa cttatggaac acacgcatgg tcggatgacg ctgaagctgg
    52561 cgcgtaatat agacctgtgc ggcctcacta ccgtcgcgcg cttcgatgat atcagcaagc
    52621 atcaattcag catcggcacc ggtgttctct tccaccgcac gctgcaggaa ttctgcccat
    52681 tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc
    52741 agttctctgt cctgggatat cacgcgttgc agactttcga cggctttggc gtattctcct
    52801 ttcgccataa acacgcgtcc catcattatg gatacgcggg cgctgttttt atctgccgcc
    52861 gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat atgctgcagg
    52921 gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc cagcttcacc
    52981 aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta gatttgtagc
    53041 aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt gaacatgtct
    53101 tccgcgcggt catataaccc ggcggccatg taatcacgcc ccagttgttg aatcgccaac
    53161 agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat gcgaatagcg
    53221 cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc ttcaacggtg
    53281 cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg ttgattacta
    53341 agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc atcttgcttg
    53401 ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac aggcaaaagc
    53461 agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg cggcactaca
    53521 gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg ttcagcgcgc
    53581 gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc gatagcaaac
    53641 cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg cgctaacaga
    53701 taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat cacgaagatc
    53761 gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat gtggttagag
    53821 cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt cgcgccgtct
    53881 gcgaaatggc atccacacgc attgttgatg atttaagggc taaattcgga aaatcaacca
    53941 gcgttaactt tcttgttctc gttgcgctat ttcgcgattt tcttccgcag gcggtgttaa
    54001 tggcccacaa attcgttgcg taagccacgt tgccaccgcc accagcgccc acgaaatcaa
    54061 cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc ttcccatgac
    54121 tcccgttgcc cagaccagca agatagcaat ggttaacgtt cgccgacgcg gccacagcaa
    54181 accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa
    54241 ccccgtctct ttctgccagt gtgaacgcaa atattgtggg atatttttct cttcagccaa
    54301 ctgttctttc actagatttc cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg
    54361 aatatgatgt gttttttcca gccagataac aaaaggtcgt ggttcctgga ctttgtcttt
    54421 gatccaggat ttaacgcctt gtcccacaag gattgccgcc gccagaatgg caaataatac
    54481 aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaata aaatcaaatg
    54541 tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg cttttagtag
    54601 ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta ctggcataac
    54661 aagcaatagt gcagctccca ctgcggtacg tcgggcaatc gaacgcatgg cctctccttt
    54721 ttgataagtc ccacaatcat aactgaaaat gccagttcca ggaaaaattg acagatttgt
    54781 gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa
    54841 taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac gtgtggcaga
    54901 agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag aactggcaac
    54961 cggacacgat catgtcgcgc tagtctatgg cgatatttcc gggcataccc cggtacttgc
    55021 gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct gcgattgtgg
    55081 cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta
    55141 tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt acgcactgca
    55201 ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg
    55261 cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag tccgcttgtt
    55321 aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata ttgttgaacg
    55381 cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata ccaaagccga
    55441 gaaaatgggc catttgctga acaaataacc ctcttgcatt gtgtaattca tttgcttgcc
    55501 ggaagcaaaa taaccggcaa gcaaatagtt gttacttcaa catattacga atgacataat
    55561 gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata cgacaacggc
    55621 agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc gtcgcgccgg
    55681 gttgtaggtt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc cctaacgttt
    55741 tacgcgttac gccttgcgga aattccagcg gcaggatgcc catgccaatt aaattcgaac
    55801 ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat accaagcaga cgcggacctt
    55861 ttgccgccca gtcacgactg gagcctgatc catactcttt cccggcaatc accgccagcg
    55921 gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg tcgctgtcag
    55981 gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg atgcgaatat
    56041 tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg taggagttaa
    56101 agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg tcgggcttaa
    56161 tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc
    56221 gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca aagaaaggcg
    56281 ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa
    56341 ttcccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg
    56401 tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata tctttcagat
    56461 aaaccggatc gcctttgcga tcatggccga taggctcaga agccaggttg atattcatat
    56521 ttcccgccag cgcataggca accaccagcg gcggcgaggc cagccagtta gttttaacca
    56581 gcggatggat acggccttca aagttacggt tgccggacag caccgcaccg acggttaaat
    56641 cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta ccaatacagg
    56701 tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt gtcagtttcg
    56761 ctgttgccag ataatcagaa acgactttcg aacccggtgc cagcgacgct ttgacccatg
    56821 gttgccgctt gaggcccaga gttacggctt ttttcgccag caagcctgcg gccatcagca
    56881 cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag
    56941 gtaactgata ctgatgtccg ttcataacat aatcgaccgc ctggcgatct ttatgcgtgg
    57001 cattcacatc cagttcgtta ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc
    57061 gatcctgtgg gcgtttaggc cctgccaggc tcgcttcaac gtcattcata tccagttcta
    57121 acgtactggt aaaaattggt tcatcgcccg ggttacgcca catgccctgc gctttggcat
    57181 atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg
    57241 ttacagcatc gattgggaag aagccacagg tggcaccata ttctggcgac atattggcaa
    57301 tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat tcgacgaatt
    57361 tccccaccac gccatgtttg cgcagcattt gggtaaccgt gagaaccagg tctgtggcgg
    57421 taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg ataagcatgg
    57481 aaaccggctg gcctaacatt gcggcttctg cttcgatgcc acccacgccc caccccagca
    57541 cgccaaggcc gttgatcata gtagtgtgcg agtcagtacc aacgagtgta tccggataag
    57601 caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat tcgaggttaa
    57661 cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac gcttgctttc
    57721 cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt acgttttctt
    57781 caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg accaggtcga
    57841 ccggtgagag cggattaacc tttgcagtat cgccgccgag gcgtttaacc gcttcgcgca
    57901 ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc acccttgccg
    57961 ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc agcgcgtgga
    58021 tatcctcttc ggtaaccgag ttaccatcct gccagcgcag caggttttcg agcaaaactt
    58081 taagtgactt gggtagacgg gtgatatcgc ccagtgattt ggcagcaagc ggcaggctgt
    58141 agtagtggta agttttatct ttggcctgca acgtgtcctt actggcttct cgtagggttg
    58201 acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc agggtgaata
    58261 ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga taaaagagga
    58321 gaaagcttgt ggcaaagcaa atgaaaaccc cgccgcagcg gggtcaagga tcagtgagta
    58381 atgatccaga ggagttcggc ccagaagagc atcgacagag aaaacgcgcc catccaggac
    58441 caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta ctttgtgttg
    58501 agataactca acctgttatt gctgcggtgt attaaaagcc ctttaattca ttaaaaatgg
    58561 gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa
    58621 aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt
    58681 tcgtttctgg ttcattgtca ctatcctggc ataatggtga cgactccagt tgctcctgac
    58741 caaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc agaaagtgca
    58801 gaaccaagaa aaaccgccag ccaggcttta cgttttaatt caggatcgcg gcggggttcc
    58861 tgatttccag aaggcattgc taacctcata taatcgccat cacttatcag caagacgttg
    58921 aacgattggt gtaattaatc atacgatgag acaaatccta aagaaacttt agtcaaaaat
    58981 ccagcgaatt tacgaatcgt ctccagtgaa taattcaatc ttttgacctg aaatagatat
    59041 ttgaaaactg gaatttgcga ggcgggtaat tagacactac tttacaggta atttacccta
    59101 taaatatgag ggataaaaat attgtgcacg gtatacaaat aagtaacaaa agagaatata
    59161 ttccggcacc ttcgctacat aaaaggtgcc gaaaataacg caagaaatta tttttccggc
    59221 agttttatat ctttaaacat gacctcaatt tcttcattag agcgcaatgc gacagccgca
    59281 tcaacgacat cacgcgttaa atgcggtgca aaacgctgaa tgaaatcata catataactg
    59341 cgcaaaaaag tactacggcg aaaaccaatt ttggttgtac tgtggctgaa gatatcgtga
    59401 gcatcaacac gcacaaggtc gggatcggcg accggatcca ccgccatgct ggcaatgacc
    59461 cctaccccca gccctaaccg gacgtaagtt ttaatgacgt cagcatccgt tgccgtgaag
    59521 acgatacgcg gcgttaaccc tgcgcggtta aaggcagtat ccagttctga acgtccggta
    59581 aagccgaagg tatatgtcac caacggatat tgcgccagtt cttcaatggt aatggctttt
    59641 ttgcctgcca gcgggtgatc cggagtgact acaatagccc gattccagtg gtagcacggt
    59701 aacatcacta aatcttcata cagatgcagc gcttctgtgg cgatagcgaa atcggcattg
    59761 cctttagaga cggcatcagc aatttgtgtc ggcgagccct ggtgcatatg caaagaaacg
    59821 cggggataac gctcaataaa gcctttgatg acgtttggta atgcgtagcg tgcctgggta
    59881 tgcgtggtgg cgatatacag tgagccttta tccggccagg tgtgctctcc ggcaaccgat
    59941 tttatggcat cgactttcga caggacttcg cgagcgatac gaattatttc ttgccctgct
    60001 ggcgttacct gcgtcaggtg cttgccgctt cgggaaaaaa tttgaatgcc tagctcgtct
    60061 tccagcattc tgacttgttt actgatcccg ggttgtgatg tgtaaagtcc ttccgctgtt
    60121 gatgagacat tcaggttatg attgaccacc tcaacaatat agcgaagttg ttgtaatttc
    60181 atgttaaacc atccacttag acgccatcag aacatcgttt tttcgtcaca ccaggcggcg
    60241 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
    60301 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
    60361 ctttgtttat aaaaaactga cagaattaaa ggttattttt tcccttcaac ccatttgcca
    60421 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
    60481 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgcggc
    60541 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata cagctcttcc
    60601 accagcggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaaaacaccc
    60661 gcagcgccgt cacgcaacac aaaataagca tccgattttt cgcaaggtag ttctggcagc
    60721 ggaaccggat cttctttcgg tggtgccact tcgccgttac gtaaaatctt acgtgtgttt
    60781 ttacactctt cgttggtgca ggccatgtat ttaccaaaac gccccatttt caggtgcatt
    60841 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
    60901 ccctcttcga tctcgtaacc gtcgcaagtt gggttattac cacagacatg caacttacgt
    60961 ttcggatcga tgagatagct gtccatcgcc gtgccacatt tcgggcaacg acgttttgcg
    61021 cgcagcgcgt tggtttcagc gtcttcgcct tccagcacgt tcagcacttc gttttccggc
    61081 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcataacc agaacagcca
    61141 aggaataccc cagtgctcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
    61201 atgctggtca gaaccatctg gttcgggcgc atacccccct cttccggatc tttttcagct
    61261 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
    61321 tcgtgatttg ccacctggtc aaggctgttt tccatctgcg cggtgaagtc gtagttcatt
    61381 aactcgcgga aattctcttc caggcgatcg gtgacgattt cgcccatttt ttccgcatag
    61441 aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat gatcgacgca
    61501 taggtagacg ggcgaccgat accacgtttt tccagttctt taaccagcga tgcttcactg
    61561 aaacgggctg gcggcttggt aaagtgctgg gctggtgtca gttcaacgag cgtcagagca
    61621 tcgcctttat cgactgccgg taagatacga tcttcatcgc ctttacgcag tgcaggcatc
    61681 actttcgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
    61741 gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggcaacgaac
    61801 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
    61861 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
    61921 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
    61981 ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
    62041 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaatcca
    62101 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
    62161 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
    62221 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcgttctg atgagtcacc
    62281 tgcaacgcca acgcttcacc agatggcgtg gtcgtgctgg catcgacttc ccagaactct
    62341 tccggcacga acgctttaat ttcacgctca cgctcgacca ccaggcggac tgccaccgac
    62401 tgcacacgac cggcagacag accacgagcg atctttttcc atagcagcgg cgaaaccata
    62461 taccccacca cacggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
    62521 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
    62581 actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca tgcaatggct
    62641 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
    62701 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
    62761 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
    62821 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
    62881 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cactacccag atacttgttg
    62941 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
    63001 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
    63061 taccgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
    63121 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
    63181 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
    63241 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
    63301 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
    63361 gagtaaataa tgaacaaaga gacacacccc atcgaccgcg aaacgctgtt gaaagaggcc
    63421 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
    63481 cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca aggattgcca
    63541 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
    63601 aaatatcacc tggttgatta aagcaaaacg cgaggtctta ccctcgcgtt tgtcttttac
    63661 atcaatggct tttgcccccg ctgccaccag cgtaacaaca atcgatcggc gctctctgcc
    63721 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtgc attgaccact
    63781 tctcggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
    63841 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
    63901 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
    63961 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtacgctta
    64021 tactgcccgg cggtgtgaag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
    64081 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
    64141 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
    64201 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
    64261 tgcaccatgc caccagggct ttccagacgt agtacaacct gatcctgcgg tttgaatgct
    64321 gcgagtacag ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
    64381 ttaaaatcca gcacccagac gcggggttta ctgtcagttg ccacctctcc cagtttggct
    64441 ttcgcttttg ctgctttcgc ttcttgctta tgctttttct tctgcgcttt gtgccactgt
    64501 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
    64561 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
    64621 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt cgccaaaaac
    64681 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
    64741 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
    64801 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
    64861 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
    64921 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
    64981 gagccagcga tgggattggc cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
    65041 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
    65101 aaactggacg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaagatt
    65161 gccaacaact ggcacagcgc attaccgtta attatccgcg tctggatggt gttttgcata
    65221 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccacag gtctggcagg
    65281 acgtcataca ggtcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
    65341 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac
    65401 gagccaactg gggcgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
    65461 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
    65521 ccgcaatgcg tgccagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
    65581 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
    65641 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
    65701 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
    65761 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
    65821 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
    65881 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
    65941 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccaccg cctgccgcga
    66001 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
    66061 tgagctgact tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
    66121 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
    66181 tcttgaactg gcggatacgg tgagtgagtt gcgccccatc aaacatgcgt ttgatgccgg
    66241 tgtaaaagcg cagataggga tcgattatta atctgtaaaa atgccggata tgacgtccgg
    66301 catactttga ttaaccgtta ttattacgtc cgccagaacg acgaccgccg ctcacctgac
    66361 tgtgacgttt caccgcacga cgaatctgat tcgccttcat gcgacgacgg tctttttcta
    66421 cagcgacttt agagctggtt tctggcggta gctccaccag ttcgcgcaga tagttagtct
    66481 gggcgaggtc cagctccgtc cagccaccgc gtggcagacc tttcggcagt gggatatcac
    66541 cgtaacgaac acggatcagg cggctgacct gcacaccaac cgcttcccac agacgacgaa
    66601 cttcacggtt acggccttcg gtcagagtaa cgttgtacca ctggttgatc ccttcgccgc
    66661 cgctgaactt gatggtttta aaagctgccg gaccatcttc cagttgcacg ccacgactca
    66721 aatcacgcag tttcgcgtcg tcaacctgac caaatacacg cacggcgtat tcacgttcaa
    66781 cttcacggct tggatgcatt aaacggtttg ccagttcacc gtcagtggtg aacagcagca
    66841 gaccacaggt gttaacgtcc agacgaccta cggcaatcca gcgtgcgcca cgcagctttg
    66901 gcagacggtc aaacaccgtc ggacgccctt ccggatcgtt acgggtacac aactcacctt
    66961 ccggtttgta ataagccaga acgcgacaaa tttgttcagc ggactcacgt accgaaataa
    67021 ggtgaccatc gatacggatt ttcaatccag gggtaacttc aacgcgatcg ccgagtttag
    67081 caattttgcc atcaacactc acacgaccgg cttcaataat agattcgatt tcacggcgag
    67141 aaccgtggcc ggcacgcgcc agcactttct gtagcttttc gctcatagag cttccttttg
    67201 gtgtcgcctt cccaggcgtc tgatatgctt ttgtgcaaca gtgagtcaga acttacatct
    67261 gactcactga tagtaaagtc tgtttgaact cttcaggcct taagtaaacg actcgacgtt
    67321 ttaactggcc tggctttgag gcgcaacata ctacactatc tttatggtta aagcttgttt
    67381 cgattaacat cagcttaatt accagccacc accgcctccg ccacccgcgc cgccgccagc
    67441 accaccaccg gaataacttc gccccaacaa actggtatag gccccgcaac ttaggtgcgt
    67501 ctgtaatgag aataaaacgc cgctacgggc aatttccggg gcgcgaacag ccgctgtgag
    67561 tgatggggtg atacgtgctg cccattctct gccaaggttt gccgagaccg cccaacctag
    67621 caacgcataa tccagtttcc gtgtttcccc tttccgacgc cgcccactgt gtgttgccgc
    67681 ctctttacgc gcaagatagt taacgatagc ttcaccgtgg gcataacgtt gctgtccagt
    67741 ttgggtaaac tttggcagaa aaatataccc catgccagtg agataaccgg tcaggcaaac
    67801 agcgctaaaa taacccgctg gcatatacca gtaaaagaat acatctccga cattgatata
    67861 gagagaatag aaggccacgc ctgaacaaat cagtggtaaa tacaaagtga tgattgcacg
    67921 attaacgcaa ggcttgtcat catcgcgggc aaataaaagc tcaagggaac aaaataaaaa
    67981 aggaataatc cagcagatca taataaacat atcgcctacc agactcattc ctgcccaacc
    68041 tacgggactc aggttaccgt agagaatgac cgccaatatt gccagtccac cccattgcaa
    68101 aaacgtatca ggccggtaaa acgaagactt tttacgcggc agataaaatt tctgcatccg
    68161 taaaaatgcc ctgcgtaaga gtctgttgcg tctccccgta agaacgacct tatcgccttt
    68221 acgaaataac gcttccagta ataactgctc tgcgggcagt tataccgcat tatttctggt
    68281 cgcgccttca ttaacacaaa tcaggatttg ctggttctta tcatactgat cttccagatg
    68341 aattttcccc ttcacgatca gatttacgat atcggcacag aaacctttat cgtcgtaaac
    68401 cagcttcgca tcgagacgta acatgccggg cgtgtaatct ggcggaatga catcggtttc
    68461 aatcacatct accggcgtaa attgcggcct gcgcttccag agatataacc atccacaaac
    68521 caggaataga cacggaaacc aaattaacaa cgatgacgtg gagggtaaaa gaatatggct
    68581 gaaaatattc gtcgtttgtg gagccggggc attgctgagt aaagcgtgag gccagcggta
    68641 gagtaccgtg aaatcttcac gataaaacgg atcgctggat tcaattcttc cgtcctcaaa
    68701 gatgcggcca tttcgatatg tgtcgccctc ttctccagta aagagatcga tttcgctaaa
    68761 tggattaccc gcgatatcag ggaacttgag tgaaaatcgg gtcttataga tttcaaacgg
    68821 ccagtggtta ccagtcacgt tccagattaa cagatctgaa tctccctcac gcaggaaagc
    68881 atttttaacc tgataatgaa tttgataatg atagtcgcct tgaggcaaga aacgttgttt
    68941 atcgcccacg acaataatca ttaacccgat atgccagtca agatgccagg gttcaggaat
    69001 accatcacgg gtgacgccaa caatatgata gttcagcagt gcagcgtccc catcaggctg
    69061 catccagagt cgtggtaaat cacgataaaa tccgtgttta atctgctgat ttcgcgcctg
    69121 aaaatgaata ttttctttga tgtcgagcac cccatccggt tgcagagtgg cttcaacatc
    69181 aaagagcgaa atcccttctt cataagaagg gatctccgtg ttttctgcat aacttagaga
    69241 tgatacaaac agccctgcta tcaacagcaa aatgcagcga aacttccctg ccatagtctt
    69301 ccatccgtgg aacgcaattt gttataagaa aggcttcaca tcacctacgc cttcacgcac
    69361 cacgaccggc gtatcatcgg taagatcaat aaccgttgtc ggtttctgcc cgagatagcc
    69421 gccgtgaata atcaaatcta cctgtttttc cagacggtct ttaatttctt cgggatcgga
    69481 ttcggtaaat tcgctgcctg gcagcattag cgaagtggaa agcatcggtt cgcccagtgc
    69541 ttcaagcaac gcctgcgcaa tcgggttaga aggcacacgc ataccgatgg ttttgcgttt
    69601 ttcctgcaac agacggcgtg gcacttcctt cgtccccttc aggatgaagg tatagttgcc
    69661 cggcgtgttg tttttcatta aacgaaacgc aacgttatca acaaatgaat aggtcgagag
    69721 ttcagaaaga tcgcgacaca tcagggtaaa gttgtgaccg tccggcagct ggcgaatacg
    69781 acaaatacgc tccatcgcgt ttttatcttc aattttacag ccgagcgcat agccggaatc
    69841 agttggataa acaatcaccc cgcctttacg cacgatctcc accgcctggt tgatcagacg
    69901 ttgctgtggg ttatcaggat gaatataaaa aaactggctc ataattccct ctctgtggtg
    69961 ttctgcggct gttcccatag ctgccagacg ccttcaacgc ctgcgggcag ccagagttta
    70021 cgacccagtt cgatccacgg gcatggctga tgaaaatcag atccttgtga tgcccataaa
    70081 tgatgctgac gcgcaagggc cgccagctgg gtacgttcat tgggcgattg ctggcactgc
    70141 gcgacttcca tcgcgtcacc gtggtgttcg gcaaaatgcg caaccaatct tttcagccat
    70201 ttagcactaa gattgtaccg cccaggatga gctagcaccg ccttaccgcc agaatgatga
    70261 atgacatcaa tagcttgttc tattgtacac cactgtggcg gaacgtatcc ggttttcccg
    70321 cgcgccagat actttttaaa gacatccgcc attgaactgg ctttgccgca ctcaacaagg
    70381 aaacgcgcaa aatgaccgcg cgtcactgcg ccgccctgcg ccagtcgttg tgcgccttcc
    70441 agcgccccag gtatttgcgc tttttcaagt ctttcggcaa tcagctgcgc ccgctgattg
    70501 cgccgttctg tctgctgcgc gaggaactca cacattagcg gatgagtaat atcaatattc
    70561 agcccgacaa tatgaatttc atgattttcc cagaccgtgg aaatttccac gccgggaata
    70621 agattcagcg ccagtcccga acgtgaaatt tcctctcttg ctggcgcgat tgcagctgtg
    70681 gtgtcatggt cggtgatcgc cagggtgccg acgcgcatct cgactgcacg gtgcaccaat
    70741 gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact
    70801 gcgtaattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat
    70861 cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat cgaactagtt
    70921 aactagtacg caagttcacg taaaaagggt atcgacaatg aaagcaattt tcgtactgaa
    70981 aggttggtgg cgcacttcct gaaacgggca gtgtattcac catgcgtaaa gcaatcagat
    71041 acccagcccg cctaatgagc gggctttttt ttgaacaaaa ttagagaata acaatgcaaa
    71101 cacaaaaacc gactctcgaa ctgctaacct gcgaaggcgc ttatcgcgac aacccgactg
    71161 cgttttttca ccagttgtgt gggaatcgtc cggcaacgct gctgctggaa tccgcagata
    71221 tcgacagcaa agatgattta aaaagcctgc tgctgataga cagtgcgctg cgcattacgg
    71281 cattaggtga caccgtcaca attcaggcac tttccggcaa tggcgaagcc ctgctggcac
    71341 tactggataa cgccctgcct gcgggtgtgg aaagtgaaca atcaccaaac tgccgtgtgc
    71401 tgcgcttccc ccctgtcagt ccactgctgg atgaagacgc ccgcttatgc tccctttcgg
    71461 tttttgacgc tttccgttta ttgcagaatc tgttgaatgt accgaaggaa gaacgagaag
    71521 ccatgttctt cggcggcctg ttctcttatg accttgtggc gggatttgaa gatttaccgc
    71581 aactgtcagc ggaaaataac tgccctgatt tctgttttta tctcgctgaa acgctgatgg
    71641 tgattgacca tcagaaaaaa agcacccgta ttcaggccag cctgtttgct ccgaatgaag
    71701 aagaaaaaca acgtctcact gctcgcctga acgaactacg tcagcaactg accgaagccg
    71761 cgccgccgct gccagtggtt tccgtgccgc atatgcgttg tgaatgtaat cagagcgatg
    71821 aagagttcgg tggcgtagtg cgtttgttgc aaaaagcgat tcgcgctgga gaaattttcc
    71881 aggtggtgcc atctcgccgt ttctctctgc cctgcccgtc accgctggcg gcctattacg
    71941 tgctgaaaaa gagtaatccc agcccgtaca tgttttttat gcaggataat gatttcaccc
    72001 tatttggcgc gtcgccggaa agctcgctca agtatgatgc caccagccgc cagattgaga
    72061 tctacccgat tgccggaaca cgcccacgcg gtcgtcgcgc cgatggttca ctggacagag
    72121 atctcgacag ccgtattgaa ctggaaatgc gtaccgatca taaagagctg tctgaacatc
    72181 tgatgctggt tgatctcgcc cgtaatgatc tggcacgcat ttgcaccccc ggcagccgct
    72241 acgtcgccga tctcaccaaa gttgaccgtt attcctatgt gatgcacctc gtctctcgcg
    72301 tagtcggcga actgcgccac gatctcgacg ccctacacgc ttaccgcgcc tgtatgaata
    72361 tggggacgtt aagcggtgcg ccgaaagtgc gcgccatgca gttaattgcc gaggcggaag
    72421 gtcgtcgccg cggcagctac ggcggcgcgg taggttattt caccgcgcac ggcgatctcg
    72481 acacctgcat tgtgatccgc tcggcgctgg tggaaaacgg tatcgccacc gtgcaagccg
    72541 gtgctggcgt agtccttgat tctattccgc agtcggaagc cgacgaaacc cgtaataaag
    72601 cccgcgctgt actgcgcgct attgccaccg cgcatcatgc acaggagact ttctgatggc
    72661 tgacatactg ctgctcgata atatcgactc ttttacgtac aacctggcag atcagttgcg
    72721 cagcaatggg cataacgtgg tgatttaccg caaccatatt ccggcgcaga ccttaactga
    72781 acgcctggcg accatgagca atccggtact gatgctttct cctggccccg gtgtgccgag
    72841 cgaagccggt tgtatgccgg aactcctcac ccgcttgcgt ggcaagctgc ccattattgg
    72901 catttgcctc ggacatcagg caattgtcga agcttacggg ggctatgtcg gtcaggcggg
    72961 cgaaattctt cacggtaaag cgtcgagcat tgaacatgac ggtcaggcga tgtttgccgg
    73021 attaacaaac ccgctgccgg tggcgcgtta tcactcgctg gttggcagta acattccggc
    73081 cggtttaacc atcaacgccc attttaatgg catggtgatg gcagtacgtc acgatgcgga
    73141 tcgcgtttgt ggattccagt tccatccgga atccattctc accacccagg gcgctcgcct
    73201 gctggaacaa acgctggcct gggcgcagca gaaactagag ccagccaaca cgctgcaacc
    73261 gattctggaa aaactgtatc aggcgcagac gcttagccaa caagaaagcc accagctgtt
    73321 ttcagcggtg gtgcgtggcg agctgaagcc ggaacaactg gcggcggcgc tggtgagcat
    73381 gaaaattcgc ggtgagcacc cgaacgagat cgccggggca gcaaccgcgc tactggaaaa
    73441 cgcagcgccg ttcccgcgcc cggattatct gtttgctgat atcgtcggta ctggcggtga
    73501 cggcagcaac agtatcaata tttctaccgc cagtgcgttt gtcgccgcgg cctgtgggct
    73561 gaaagtggcg aaacacggca accgtagcgt ctccagtaaa tctggttcgt ccgatctgct
    73621 ggcggcgttc ggtattaatc ttgatatgaa cgccgataaa tcgcgccagg cgctggatga
    73681 gttaggtgta tgtttcctct ttgcgccgaa gtatcacacc ggattccgcc acgcgatgcc
    73741 ggttcgccag caactgaaaa cccgcaccct gttcaatgtg ctggggccat tgattaaccc
    73801 ggcgcatccg ccgctggcgt taattggtgt ttatagtccg gaactggtgc tgccgattgc
    73861 cgaaaccttg cgcgtgctgg ggtatcaacg cgcggcggtg gtgcacagcg gcgggatgga
    73921 tgaagtttca ttacacgcgc cgacaatcgt tgccgaactg catgacggcg aaattaaaag
    73981 ctatcagctc accgcagaag actttggcct gacaccctac caccaggagc aactggcagg
    74041 cggaacaccg gaagaaaacc gtgacatttt aacacgtttg ttacaaggta aaggcgacgc
    74101 cgcccatgaa gcagccgtcg ctgcgaacgt cgccatgtta atgcgcctgc atggccatga
    74161 agatctgcaa gccaatgcgc aaaccgttct tgaggtactg cgcagtggtt ccgcttacga
    74221 cagagtcacc gcactggcgg cacgagggta aatgatgcaa accgttttag cgaaaatcgt
    74281 cgcagacaag gcgatttggg tagaagcccg caaacagcag caaccgctgg ccagttttca
    74341 gaatgaggtt cagccgagca cgcgacattt ttatgatgcg ctacagggtg cgcgcacggc
    74401 gtttattctg gagtgcaaga aagcgtcgcc gtcaaaaggc gtgatccgtg atgatttcga
    74461 tccagcacgc attgccgcca tttataaaca ttacgcttcg gcaatttcgg tgctgactga
    74521 tgagaaatat tttcagggga gctttgattt cctccccatc gtcagccaaa tcgccccgca
    74581 gccgatttta tgtaaagact tcattatcga cccttaccag atctatctgg cgcgctatta
    74641 ccaggccgat gcctgcttat taatgctttc agtactggat gacgaccaat atcgccagct
    74701 tgccgccgtc gctcacagtc tggagatggg ggtgctgacc gaagtcagta atgaagagga
    74761 acaggagcgc gccattgcat tgggagcaaa ggtcgttggc atcaacaacc gcgatctgcg
    74821 tgatttgtcg attgatctca accgtacccg cgagcttgcg ccgaaactgg ggcacaacgt
    74881 gacggtaatc agcgaatccg gcatcaatac ttacgctcag gtgcgcgagt taagccactt
    74941 cgctaacggt tttctgattg gttcggcgtt gatggcccat gacgatttgc acgccgccgt
    75001 gcgccgggtg ttgctgggtg agaataaagt atgtggcctg acgcgtgggc aagatgctaa
    75061 agcagcttat gacgcgggcg cgatttacgg tgggttgatt tttgttgcga catcaccgcg
    75121 ttgcgtcaac gttgaacagg cgcaggaagt gatggctgcg gcaccgttgc agtatgttgg
    75181 cgtgttccgc aatcacgata ttgccgatgt ggtggacaaa gctaaggtgt tatcgctggc
    75241 ggcagtgcaa ctgcatggta atgaagaaca gctgtatatc gatacgctgc gtgaagctct
    75301 gccagcacat gttgccatct ggaaagcatt aagcgtcggt gaaaccctgc ccgcccgcga
    75361 gtttcagcac gttgataaat atgttttaga caacggccag ggtggaagcg ggcaacgttt
    75421 tgactggtca ctattaaatg gtcaatcgct tggcaacgtt ctgctggcgg ggggcttagg
    75481 cgcagataac tgcgtggaag cggcacaaac cggctgcgcc ggacttgatt ttaattctgc
    75541 tgtagagtcg caaccgggca tcaaagacgc acgtcttttg gcctcggttt tccagacgct
    75601 gcgcgcatat taaggaaagg aacaatgaca acattactta acccctattt tggtgagttt
    75661 ggcggcatgt acgtgccaca aatcctgatg cctgctctgc gccagctgga agaagctttt
    75721 gtcagtgcgc aaaaagatcc tgaatttcag gctcagttca acgacctgct gaaaaactat
    75781 gccgggcgtc caaccgcgct gaccaaatgc cagaacatta cagccgggac gaacaccacg
    75841 ctgtatctca agcgtgaaga tttgctgcac ggcggcgcgc ataaaactaa ccaggtgctg
    75901 gggcaggcgt tgctggcgaa gcggatgggt aaaaccgaaa tcatcgccga aaccggtgcc
    75961 ggtcagcatg gcgtggcgtc ggcccttgcc agcgccctgc tcggcctgaa atgccgtatt
    76021 tatatgggtg ccaaagacgt tgaacgccag tcgcctaacg tttttcgtat gcgcttaatg
    76081 ggtgcggaag tgatcccggt gcatagcggt tccgcgacgc tgaaagatgc ctgtaacgag
    76141 gcgctgcgcg actggtccgg tagttacgaa accgcgcact atatgctggg caccgcagct
    76201 ggcccgcatc cttatccgac cattgtgcgt gagtttcagc ggatgattgg cgaagaaacc
    76261 aaagcgcaga ttctggaaag agaaggtcgc ctgccggatg ccgttatcgc ctgtgttggc
    76321 ggcggttcga atgccatcgg catgtttgct gatttcatca atgaaaccaa cgtcggcctg
    76381 attggtgtgg agccaggtgg tcacggtatc gaaactggcg agcacggcgg accgctaaaa
    76441 catggtcgcg tgggtatcta tttcggtatg aaagcgccga tgatgcaaac cgaagacggg
    76501 cagattgaag aatcttactc catctccgcc ggactggatt tcccgtctgt cggcccacaa
    76561 cacgcgtatc ttaacagcac tggacgcgct gattacgtgt ctattaccga tgatgaagcc
    76621 cttgaagcct tcaaaacgct gtgcctgcac gaagggatca tcccggcgct ggaatcctcc
    76681 cacgccctgg cccatgcgtt gaaaatgatg cgcgaaaacc cggataaaga gcagctactg
    76741 gtggttaacc tttccggtcg cggcgataaa gacatcttca ccgttcacga tattttgaaa
    76801 gcacgagggg aaatctgatg gaacgctacg aatctctgtt tgcccagttg aaggagcgca
    76861 aagaaggcgc attcgttcct ttcgtcacgc tcggtgatcc gggcattgag cagtcattga
    76921 aaattatcga tacgctaatt gaagccggtg ctgacgcgct ggagttaggt atccccttct
    76981 ccgacccact ggcggatggc ccgacgattc aaaacgccac tctgcgcgcc tttgcggcag
    77041 gtgtgactcc ggcacaatgt tttgaaatgc tggcactgat tcgccagaaa cacccgacca
    77101 ttcccattgg cctgttgatg tatgccaatc tggtgtttaa caaaggcatt gatgagtttt
    77161 atgcccagtg cgaaaaagtc ggcgtcgatt cggtgctggt tgccgatgtg ccagttgaag
    77221 agtccgcgcc cttccgccag gccgcgttgc gtcataatgt cgcacctatc ttcatctgcc
    77281 cgccaaatgc cgatgacgac ctgctgcgcc agatagcctc ttacggtcgt ggttacacct
    77341 atttgctgtc acgagcaggc gtgaccggcg cagaaaaccg cgccgcgtta cccctcaatc
    77401 atctggttgc gaagctgaaa gagtacaacg ctgcacctcc attgcaggga tttggtattt
    77461 ccgccccgga tcaggtaaaa gcagcgattg atgcaggagc tgcgggcgcg atttctggtt
    77521 cggccattgt taaaatcatc gagcaacata ttaatgagcc agagaaaatg ctggcggcac
    77581 tgaaagtttt tgtacaaccg atgaaagcgg cgacgcgcag ttaatcccac agccgccagt
    77641 tccgctggcg gcattttaac tttctttaat gaagccggaa aaatcctaaa ttcatttaat
    77701 atttatcttt ttaccgtttc gcttaccccg gtcgaacgtc aacttacgtc atttttccgc
    77761 ccaacagtaa tataatcaaa caaattaatc ccgca
//
LOCUS       DAJOSY010000008        75302 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJOSY010000008 DAJOSY010000000
VERSION     DAJOSY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.008"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            55..927
                     /locus_tag="OHN27_000592"
     CDS             55..927
                     /locus_tag="OHN27_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCQ2285093.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSDSGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(987..2285)
                     /gene="eno"
                     /locus_tag="OHN27_000593"
     CDS             complement(987..2285)
                     /gene="eno"
                     /locus_tag="OHN27_000593"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCQ2285094.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2373..4010)
                     /gene="pyrG"
                     /locus_tag="OHN27_000594"
     CDS             complement(2373..4010)
                     /gene="pyrG"
                     /locus_tag="OHN27_000594"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCQ2285095.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4238..5029)
                     /gene="mazG"
                     /locus_tag="OHN27_000595"
     CDS             complement(4238..5029)
                     /gene="mazG"
                     /locus_tag="OHN27_000595"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCQ2285096.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5100..5435)
                     /gene="mazF"
                     /locus_tag="OHN27_000596"
     CDS             complement(5100..5435)
                     /gene="mazF"
                     /locus_tag="OHN27_000596"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCQ2285097.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5435..5683)
                     /gene="mazE"
                     /locus_tag="OHN27_000597"
     CDS             complement(5435..5683)
                     /gene="mazE"
                     /locus_tag="OHN27_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCQ2285098.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5761..7995)
                     /locus_tag="OHN27_000598"
     CDS             complement(5761..7995)
                     /locus_tag="OHN27_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCQ2285099.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8043..9344)
                     /gene="rlmD"
                     /locus_tag="OHN27_000599"
     CDS             complement(8043..9344)
                     /gene="rlmD"
                     /locus_tag="OHN27_000599"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCQ2285100.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARYNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9401..12157
                     /gene="barA"
                     /locus_tag="OHN27_000600"
     CDS             9401..12157
                     /gene="barA"
                     /locus_tag="OHN27_000600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCQ2285101.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12389..13726)
                     /gene="gudD"
                     /locus_tag="OHN27_000601"
     CDS             complement(12389..13726)
                     /gene="gudD"
                     /locus_tag="OHN27_000601"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCQ2285102.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLMNLSGAHAPFFTRNIVIIKD
                     NSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQT
                     FDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKATP
                     LPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAES
                     IVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMAE
                     FRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGLT
                     WGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQVP
                     EKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13728..15080)
                     /gene="gudP"
                     /locus_tag="OHN27_000602"
     CDS             complement(13728..15080)
                     /gene="gudP"
                     /locus_tag="OHN27_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCQ2285103.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNKKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15515..15964)
                     /locus_tag="OHN27_000603"
     CDS             complement(15515..15964)
                     /locus_tag="OHN27_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCQ2285104.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(15982..16764)
                     /gene="truC"
                     /locus_tag="OHN27_000604"
     CDS             complement(15982..16764)
                     /gene="truC"
                     /locus_tag="OHN27_000604"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCQ2285105.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(16764..17093)
                     /locus_tag="OHN27_000605"
     CDS             complement(16764..17093)
                     /locus_tag="OHN27_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCQ2285106.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(17715..18260)
                     /gene="syd"
                     /locus_tag="OHN27_000606"
     CDS             complement(17715..18260)
                     /gene="syd"
                     /locus_tag="OHN27_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCQ2285107.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18328..19176
                     /gene="queF"
                     /locus_tag="OHN27_000607"
     CDS             18328..19176
                     /gene="queF"
                     /locus_tag="OHN27_000607"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCQ2285108.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19288..20652
                     /gene="ppnN"
                     /locus_tag="OHN27_000608"
                     /gene_synonym="ygdH"
     CDS             19288..20652
                     /gene="ppnN"
                     /locus_tag="OHN27_000608"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCQ2285109.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21209..22498
                     /gene="sdaC"
                     /locus_tag="OHN27_000609"
     CDS             21209..22498
                     /gene="sdaC"
                     /locus_tag="OHN27_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCQ2285110.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22556..23923
                     /gene="sdaB"
                     /locus_tag="OHN27_000610"
     CDS             22556..23923
                     /gene="sdaB"
                     /locus_tag="OHN27_000610"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCQ2285111.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24035..24790
                     /gene="xni"
                     /locus_tag="OHN27_000611"
     CDS             24035..24790
                     /gene="xni"
                     /locus_tag="OHN27_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCQ2285112.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(24845..25993)
                     /gene="fucO"
                     /locus_tag="OHN27_000612"
     CDS             complement(24845..25993)
                     /gene="fucO"
                     /locus_tag="OHN27_000612"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCQ2285113.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26021..26668)
                     /gene="fucA"
                     /locus_tag="OHN27_000613"
     CDS             complement(26021..26668)
                     /gene="fucA"
                     /locus_tag="OHN27_000613"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCQ2285114.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27215..28531
                     /gene="fucP"
                     /locus_tag="OHN27_000614"
     CDS             27215..28531
                     /gene="fucP"
                     /locus_tag="OHN27_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCQ2285115.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            28564..30339
                     /gene="fucI"
                     /locus_tag="OHN27_000615"
     CDS             28564..30339
                     /gene="fucI"
                     /locus_tag="OHN27_000615"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCQ2285116.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            30448..31866
                     /gene="fucK"
                     /locus_tag="OHN27_000616"
     CDS             30448..31866
                     /gene="fucK"
                     /locus_tag="OHN27_000616"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCQ2285117.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            31868..32290
                     /gene="fucU"
                     /locus_tag="OHN27_000617"
     CDS             31868..32290
                     /gene="fucU"
                     /locus_tag="OHN27_000617"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCQ2285118.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32348..33079
                     /gene="fucR"
                     /locus_tag="OHN27_000618"
     CDS             32348..33079
                     /gene="fucR"
                     /locus_tag="OHN27_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCQ2285119.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33123..34223)
                     /gene="rlmM"
                     /locus_tag="OHN27_000619"
     CDS             complement(33123..34223)
                     /gene="rlmM"
                     /locus_tag="OHN27_000619"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCQ2285120.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34216..34611)
                     /locus_tag="OHN27_000620"
     CDS             complement(34216..34611)
                     /locus_tag="OHN27_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCQ2285121.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(34630..35547)
                     /gene="gcvA"
                     /locus_tag="OHN27_000621"
     CDS             complement(34630..35547)
                     /gene="gcvA"
                     /locus_tag="OHN27_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCQ2285122.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(35898..36125)
                     /locus_tag="OHN27_000622"
     CDS             complement(35898..36125)
                     /locus_tag="OHN27_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCQ2285123.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36317..37522
                     /gene="csdA"
                     /locus_tag="OHN27_000623"
     CDS             36317..37522
                     /gene="csdA"
                     /locus_tag="OHN27_000623"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCQ2285124.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIASVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37522..37965
                     /gene="csdE"
                     /locus_tag="OHN27_000624"
     CDS             37522..37965
                     /gene="csdE"
                     /locus_tag="OHN27_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCQ2285125.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38016..38822)
                     /gene="tcdA"
                     /locus_tag="OHN27_000625"
     CDS             complement(38016..38822)
                     /gene="tcdA"
                     /locus_tag="OHN27_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCQ2285126.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39061..40158)
                     /gene="mltA"
                     /locus_tag="OHN27_000626"
     CDS             complement(39061..40158)
                     /gene="mltA"
                     /locus_tag="OHN27_000626"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCQ2285127.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40367..40443
                     /locus_tag="OHN27_000627"
     tRNA            40367..40443
                     /locus_tag="OHN27_000627"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40401..40403,aa:Met,seq:cat)
     gene            40477..40553
                     /locus_tag="OHN27_000628"
     tRNA            40477..40553
                     /locus_tag="OHN27_000628"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40511..40513,aa:Met,seq:cat)
     gene            40587..40663
                     /locus_tag="OHN27_000629"
     tRNA            40587..40663
                     /locus_tag="OHN27_000629"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40621..40623,aa:Met,seq:cat)
     gene            complement(40738..41991)
                     /gene="amiC"
                     /locus_tag="OHN27_000630"
     CDS             complement(40738..41991)
                     /gene="amiC"
                     /locus_tag="OHN27_000630"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCQ2285128.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42223..43554
                     /gene="argA"
                     /locus_tag="OHN27_000631"
     CDS             42223..43554
                     /gene="argA"
                     /locus_tag="OHN27_000631"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCQ2285129.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43616..45442)
                     /gene="recD"
                     /locus_tag="OHN27_000632"
     CDS             complement(43616..45442)
                     /gene="recD"
                     /locus_tag="OHN27_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCQ2285130.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLSDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(45442..48984)
                     /gene="recB"
                     /locus_tag="OHN27_000633"
     CDS             complement(45442..48984)
                     /gene="recB"
                     /locus_tag="OHN27_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCQ2285131.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQEQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQIGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVASVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(48977..51865)
                     /gene="ptrA"
                     /locus_tag="OHN27_000634"
     CDS             complement(48977..51865)
                     /gene="ptrA"
                     /locus_tag="OHN27_000634"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCQ2285132.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52041..55409)
                     /gene="recC"
                     /locus_tag="OHN27_000635"
     CDS             complement(52041..55409)
                     /gene="recC"
                     /locus_tag="OHN27_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCQ2285133.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55422..55745)
                     /locus_tag="OHN27_000636"
     CDS             complement(55422..55745)
                     /locus_tag="OHN27_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCQ2285134.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55730..56137)
                     /locus_tag="OHN27_000637"
     CDS             complement(55730..56137)
                     /locus_tag="OHN27_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCQ2285135.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(56134..56697)
                     /locus_tag="OHN27_000638"
     CDS             complement(56134..56697)
                     /locus_tag="OHN27_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCQ2285136.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTGTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56688..57158)
                     /locus_tag="OHN27_000639"
     CDS             complement(56688..57158)
                     /locus_tag="OHN27_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCQ2285137.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57343..58137)
                     /gene="thyA"
                     /locus_tag="OHN27_000640"
     CDS             complement(57343..58137)
                     /gene="thyA"
                     /locus_tag="OHN27_000640"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCQ2285138.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58144..59019)
                     /gene="lgt"
                     /locus_tag="OHN27_000641"
     CDS             complement(58144..59019)
                     /gene="lgt"
                     /locus_tag="OHN27_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCQ2285139.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59170..61416)
                     /gene="ptsP"
                     /locus_tag="OHN27_000642"
     CDS             complement(59170..61416)
                     /gene="ptsP"
                     /locus_tag="OHN27_000642"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCQ2285140.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61429..61959)
                     /gene="rppH"
                     /locus_tag="OHN27_000643"
     CDS             complement(61429..61959)
                     /gene="rppH"
                     /locus_tag="OHN27_000643"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCQ2285141.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            62644..63333
                     /gene="mutH"
                     /locus_tag="OHN27_000644"
     CDS             62644..63333
                     /gene="mutH"
                     /locus_tag="OHN27_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCQ2285142.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALVGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63402..64115
                     /locus_tag="OHN27_000645"
     CDS             63402..64115
                     /locus_tag="OHN27_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCQ2285143.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64253..64471
                     /gene="ygdR"
                     /locus_tag="OHN27_000646"
     CDS             64253..64471
                     /gene="ygdR"
                     /locus_tag="OHN27_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCQ2285144.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            64579..65619
                     /locus_tag="OHN27_000647"
     CDS             64579..65619
                     /locus_tag="OHN27_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCQ2285145.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(65651..66844)
                     /gene="lplT"
                     /locus_tag="OHN27_000648"
     CDS             complement(65651..66844)
                     /gene="lplT"
                     /locus_tag="OHN27_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCQ2285146.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(66837..68996)
                     /gene="aas"
                     /locus_tag="OHN27_000649"
     CDS             complement(66837..68996)
                     /gene="aas"
                     /locus_tag="OHN27_000649"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCQ2285147.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            69582..70613
                     /gene="galR"
                     /locus_tag="OHN27_000650"
     CDS             69582..70613
                     /gene="galR"
                     /locus_tag="OHN27_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCQ2285148.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(70620..71882)
                     /gene="lysA"
                     /locus_tag="OHN27_000651"
     CDS             complement(70620..71882)
                     /gene="lysA"
                     /locus_tag="OHN27_000651"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCQ2285149.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALATDGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            72004..72939
                     /gene="lysR"
                     /locus_tag="OHN27_000652"
     CDS             72004..72939
                     /gene="lysR"
                     /locus_tag="OHN27_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCQ2285150.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(72926..73618)
                     /gene="ygeA"
                     /locus_tag="OHN27_000653"
     CDS             complement(72926..73618)
                     /gene="ygeA"
                     /locus_tag="OHN27_000653"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCQ2285151.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(73747..75165)
                     /gene="araE"
                     /locus_tag="OHN27_000654"
     CDS             complement(73747..75165)
                     /gene="araE"
                     /locus_tag="OHN27_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCQ2285152.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGI"
BASE COUNT        18398 a        20282 c        18416 g        18206 t
ORIGIN      
        1 aaattaccga tgccgccgtc aatttattta tccagattta atgagaaatt tataatgcgc
       61 tatttcatac taatgttcac tttcgtatgt tcctttgttg cagcccaacc aactattgtc
      121 ccacaattac agcaacaagt tacggatctt acgagtagct taaactcaca agaaaagaaa
      181 gaactgacgc acaagttaga atctattttc aataataccc aagtgcaaat tgccgtatta
      241 attgttccca caaccaaaga cgagacaatt gaacaatatg ctacaagagt ttttgacaat
      301 tggcgtttag gagatgccaa acgtaatgat gggatactga tcgttgttgc ctggtcggat
      361 cgcactgtcc gcatccaggt aggttatggg ctggaagaaa aggtaaccga tgctctggct
      421 ggagatatca tccgtagcaa catgataccc gcatttaaac aacaaaaatt agctaaggga
      481 ttagagctag ctataaacgc tttgaataac caactcactt cacaacacca atatccgact
      541 aatccttcag aaagtgaatc agcgtcttcc agtgatcatt attactttgc tattttttgg
      601 gtatttgcag tgatgttctt ccctttctgg ttttttcatc aaggcagtaa tttttgtcgc
      661 gcatgtaaaa gtggagtctg tatttcagca atctatcttt tagatttatt cctgttctcc
      721 gataaaattt tttccattgc tgtattttcc ttctttttta ctttcaccat atttatggtc
      781 tttacctgtt tatgtgtact tcagaaaaga gcatctggta gaagttatca ttcagacaac
      841 agcggttccg ctggaggctc agatagcggc ggatttagcg gcgggggcgg ttcttctgga
      901 ggtggcggcg catccggccg ctggtaactc aaccataaaa aatgccagcc cggaggctgg
      961 catttttaaa tcagataaag tcagtcttat gcctggcctt tgatctcttt acgaccgttg
     1021 tacggtgctt tttcgcccag agcttcttcg atacgaatca gctggttgta tttagcaaca
     1081 cggtcagaac ggctcataga accagttttg atctggcctg cagcagtacc aacagccagg
     1141 tcagcgatgg tagcgtcttc agtttcgcca gaacggtgag agataactgc agtgtagcca
     1201 gcatctttcg ccatcttgat tgcagccaga gtttcggtca gagaaccgat ctggttgaat
     1261 ttgatcagga tggagttagc gatacctttt tcgatacctt ctttcaggat cttggtgttg
     1321 gttacgaaca ggtcgtcacc aaccagctgg attttgtcgc ccagaacttt ggtctggtat
     1381 gcgaaaccgt cccagtcaga ttcgtccaga ccgtcttcga tagaaacgat cgggtactgt
     1441 ttggtcagtt cttccaggaa gtgagtgaat tcttcagagg tgaacgcttt gttgccttcg
     1501 ccagccagaa cgtatttacc atctttgtag aattcagaag ctgcgcagtc catcgccaaa
     1561 gtgatgtctt tgcccagttc ataaccagca gctttaacag cttcagcgat aacagccaga
     1621 gcttcagcgt tggaacccag gttcggcgca tagccacctt cgtcaccaac agcagtgttc
     1681 atgcctttcg ctttcagaac ttttgccagg tgatggaaaa cttcagaacc catgcggatg
     1741 gcttctttca cagttttcgc gccaaccggc tgaatcatga attcctggat atcaacgttg
     1801 ttgtcagcgt gctcaccacc gttgatgatg ttcatcatcg gaaccggcat agagtatttg
     1861 cccggagtac cgttcagttc agcgatgtgc tcgtacagcg gcataccttt agcagctgca
     1921 gcagctttgg cgttagccag agatacagcc aggattgcgt tcgcgccgaa tttggatttg
     1981 ttttcggtgc cgtccaggtc gatcatgatc ttgtcaatgc cagcctgatc tttagcatct
     2041 ttgccaatca gcgcctgagc gatcgggccg tttaccgcag caacagcttt ggttacgcct
     2101 ttacccagga aacgggattt gtcgccatcg cgcagttcca gagcttcacg ggaaccagta
     2161 gaagcacctg acggagcagc tgccataccg acgaaaccac cctccagatg tacttcggct
     2221 tcaacagtcg ggttaccacg ggagtcgatg atttcacgac cgatgatttt tacgattttg
     2281 gacattaggt tttcctcaag tcactagtta aactgaaact ccagacaaac aacgcgtacc
     2341 cagggtacgc gttgccgctc taactttttt acttacttcg cctgacgttt ctggaactcg
     2401 ctggcggctt tcacaaagcc tgcaaacagc gggtgaccat cacgtggagt agaagtaaac
     2461 tccggatgga actggcaagc cacgaaccac gggtgattcg gaacttcgat gatctcgacc
     2521 aactgatcat ccccggaacg gcccgcaacg cgcagacctg catcttcaat ctgtttcaac
     2581 agcatgttgt tgacttcgta acggtgacga tgacgctcaa caattgtcgg cgcattgtac
     2641 agctggcgaa ccaggctatc gtcaaccaac tggcactgct gtgcgccgag acgcatggta
     2701 ccgccgagat cgctcttctc gctacgaact tcaacgttgc cgttttcatc gcgccactcg
     2761 gtaatcagcg ccacaaccgg gtacttacag tctggcacaa attccgtaga gttggcgttc
     2821 tccatgttgg caacatggcg agcgtaatca attaacgcca cctgcatacc caggcaaatg
     2881 cccagataag gaatattgtt ctcacgcgca aaacgcgcgg tcgtaatcat gccttctacg
     2941 ccacgatagc cgaaaccgcc aggtacgagg attgcgtcca gacctttaag gatttcaacg
     3001 ccgcgcgttt caacatcttg tgaatcgatc agtttgatgt tgacgctgac acgattcttc
     3061 agcccaccgt gtttcagtgc ttcgatcact gatttataag catccggcag ttcaatgtac
     3121 ttgccgacca taccgatggt gacttcactt accgggttcg cttcttcgaa gataacctgt
     3181 tcccattcgg acagattcgc ttccgggcag tttaagctga atcgtttaca aatataatcg
     3241 tccagcccct gagatttcaa caggcccgga attttataga tggaatcgac gtctttcaga
     3301 gaaataaccg ctttttccgg aacattacag aacaatgcaa tcttcgcacg ttcgttcgcc
     3361 ggaacagcgc gatctgaacg acaaatcagg atgtcaggct ggataccgat ggagagcagc
     3421 tcttttacag agtgctgagt cggtttggtt ttgacttcac cagacgctgc catgtacggc
     3481 accagcgtca ggtgcataaa cagagtgtgc tcacggccaa tttcaacagc catctggcga
     3541 atcgcttcga ggaacggcaa ggattcgata tcacctactg taccgccgat ttctaccagt
     3601 actacgtcat gaccttcgcc accttccagc acgcgctctt tgattgcgtt agtgatgtgc
     3661 ggaataacct gcacggttgc gccgaggtag tcaccgcggc gttctttacg cagaacgtca
     3721 gagtagatac gacccgtggt gaagttgttg cggcggctca ttttggtacg aatgaaacgc
     3781 tcgtagtgcc ccaggtccag gtcggtttca gcgccgtctt cagtaacgaa cacttccccg
     3841 tgttggattg ggctcatagt acctggatcg acgttgatgt acggatccag tttcatgatg
     3901 gtcacattga ggccacgggc ttcaagaatg gctgcgaggg aggctgcggc aatgccttta
     3961 cccagagagg atacgacccc gccggtcaca aaaatatagt tcgttgtcat gctgaacctg
     4021 agaagttagg ttgaaaagac gatggaataa ccaggacggg aaagcagtat acccgaacat
     4081 gacctgtgcc acaaactttc attatccctc ctcttcgcca gcgcactatt gaaatcagga
     4141 gtgagaaaat agcccctttg gggtaaatgt ttttgacgca aatcaagcgc ttgtcattta
     4201 aaaaatgaca caaatggcgc ttgaccgcgt aattccctta gagatcaatt tcctgccgtt
     4261 ttacctgttg ccagacttct tccattgttt cgaggtcaac acctgtcatt tccagtccac
     4321 gcgcggcaac aatacgctcc acttcgcgaa aacgacgctc gaatttttcg ttcgcttttt
     4381 gcaatgcgat ttctgctttc gtccctaaat ggcgagccag attaaccgtg gcaaacagca
     4441 ggtcccccat ttcctcctcc agtttagcct ggtcgacaac agcctgccgc gcttcgtaca
     4501 tcacctcgtc gatctcttcg tagactttat cgactaccgg accaagcgtc gtccaatcga
     4561 agccaacgtt ggcgcaacgt ttctggattt tttgcgcacg cattaaagcc ggtaaactac
     4621 gaggaatatc gtccagcgcc gaatgctgcg ctttctgcgc gcgctcttcg gttttgattt
     4681 gctcccaacg ggcaagcact tcactactgt tttcggcaga actatcagca aaaacatgcg
     4741 gatggcgacg ctctaattta tcgctaatag cagcgcaaat atcattaaag tcaaagcgcc
     4801 cttcttcctg agccatttgc gcgtaaaaca ccacctggaa tagcagatcg cccagttcac
     4861 cgcgaagatc gtcaaaatct tcacgggcga tggcgtccag cacttcgtag gtttcttcaa
     4921 gggtgtaagg cgcaatggtg gcaaatgtct gctctttatc ccacgggcag ccgttttccg
     4981 gatcgcgcag gcgctgcata atagtgagca aacggtcgat ttgattcatt gaattgtcct
     5041 gaaaattgcg ggtctgtcag gtggaaacct gtgaccagaa tagaagtgag ttagtaacac
     5101 tacccaatca gtacgttaat tttggcttta atgagttgta attcctctgg ggcaactgtt
     5161 cctttcttcg ttgctcctct tgcccgccag gcgatacttt ttacctgatc agctaacgct
     5221 acgccatcac gttcctgacc ggataaaaca acttcgaacg gatatccttt tgattgcgtt
     5281 gtacaaggaa cacacagaca catacctgtt ttgttgttgt acatgaaagg actcaggaca
     5341 acagctggac gatgtccagc ttgctcgcta ccttttgtcg ggtcaaaatc aacccaaatc
     5401 agatcgccca tatcgggtac gtatcggctt accattacca gacttcctta tctttcggct
     5461 ctccccagtc gatattctcg tggaggtttt ccggcgtgat gtcgttgacc agttcagcaa
     5521 gcgtaaatac gggctcttta cgcactggct caataattaa tttgccatcc accaggtcaa
     5581 tcttcacttc atcatcaata ttcagattga gcgcctgcat taacgtagcc gggatccgca
     5641 ccgccggtga atttccccaa cgctttacgc tactgtggat cataaccctt tcctcaaacc
     5701 gctatcatat gtagatacag tatatatcaa tctacattgt agatacgagc aaatttcggc
     5761 ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga gtttacccag
     5821 cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg tcgccagttg
     5881 ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt tggcgagaat
     5941 ggtcgtgata tcacgtaaca acccactacg atcattagct accacgcgga ccaccagcga
     6001 atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg gcgcatggga
     6061 gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc gcccctgggt
     6121 aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga tgtggtgcat
     6181 caggttgcca acaccttcga ctaccacgcg accgttatct ttactgcggt tttgcggcgt
     6241 gtagcttttt tgctgaagtt gcttcagcgc ggcggcgtcc tgctcttcgg cactcggctt
     6301 attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc cgccaccaat
     6361 cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat gtttttctgc
     6421 ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc gcccagccag
     6481 aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac gcccacggct
     6541 ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg gctgtttctg
     6601 ggtgataatt tcaatctggt cgcccatctg cagctggtag gtgaacggca caatgcgccc
     6661 gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt aagcgaagtc
     6721 cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa agacgtacac
     6781 ccggtcgtca aagacctgac tacgtacttc gtcgagcatt tcgccggaat cagccatctc
     6841 ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc ccgaacgtgc
     6901 gccgccagca gccgcgccct ctttatattt ccagtgcgca gcaacaccca actctgcatc
     6961 ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg gccccagaac
     7021 cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat cgtcaaactc
     7081 atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcatagc aatcctgtaa
     7141 acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcaaagg cgaggttctt
     7201 tttctgcatt ttacgccaga tgctgtagat gtgtttcgga cgaccataca cttccgcttt
     7261 aacgccttca gctttcatct cagcgcgcag atgaccaacg aactcttcga tgtagtgttc
     7321 gcggtcgaga cgccgttcat gcagcagttt ggcaattcgt ttgtattcgg ttggatggag
     7381 gtaacggaag cagtaatctt ccagttccca tttcagttgt ccgattccga gacggttagc
     7441 cagcggtgcg tagatgttgg tacactcttt tgccgccagt acacgttcat cttccggcgc
     7501 atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta cgcagcgaaa
     7561 atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg aggaaacaga
     7621 atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc cgtgaataag
     7681 gttaacgacc gacttaccga cgctctcacg cagcacatct tcgctgacta cgttggcatc
     7741 cgccagaggg aaaagcagcg ccgcccgcag cgtgtcaatg tccatactta atgtcgagag
     7801 gatctccacc atctcaacac cacgccacaa taacaggctg gcatccggat gcccctgcgt
     7861 ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct ggctggtaat
     7921 acccagactt gcgatccatt tttccggatc aaattcacca gccttattga tatgtgcact
     7981 tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc ggcaaatcgc
     8041 aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg tgggaacata
     8101 tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc gctatcccga
     8161 gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag ttttataatt
     8221 tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt atcgaagccg
     8281 tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata aaacgtcaca
     8341 ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc cggaacacct
     8401 tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt gcccataccg
     8461 cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa cgcacgcgct
     8521 accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct aaaagttaag
     8581 cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag tatctcacta
     8641 tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag tttttcgcga
     8701 tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct ggttgcctgt
     8761 accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca tgccctgact
     8821 ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac gtcgacaatg
     8881 tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg taagtagttc
     8941 agacttaaac gcgcgcgacg gcgatagccc cagggaacat cggcgatcac ttcagagaca
     9001 tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa atccacgctg
     9061 gcgtgttgtt gctgacagcc accgcatacg ccaaaatgag gacagcgtgg cgtttcgcgt
     9121 tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt tttatcttca
     9181 gtaacagtaa cttccgcgtt ttcctgcggc aataatccgg ggataaatag cgttttgccg
     9241 ttatatcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac ggttatgatc
     9301 tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga gactttctca
     9361 atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact acagcctgcg
     9421 cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat tgctgagtat
     9481 ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg ccggtgccag
     9541 cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa atcgcgaatc
     9601 tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc gcgcgatttc
     9661 ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg atccctcatc
     9721 aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc gtgacggcga
     9781 tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg aatcgcccag
     9841 tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgctggagc tggatcttaa
     9901 atcggttcgc ttgcagcaat ataaagagat ctttatttcc agcgtgatga tgctgttttg
     9961 tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg gtccgattcg
    10021 caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc gggtggaagg
    10081 atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg caatgtcgct
    10141 ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg atctgcgtga
    10201 aacgctggag cagatggaaa ttcagaacgt tgagttagat ctggcgaaaa agcgcgccca
    10261 ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc tgcgtacacc
    10321 actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa caccaacgca
    10381 gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa ttattaatga
    10441 tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta ttccattccc
    10501 actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc acgataaagg
    10561 gttagaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg gcgacccgct
    10621 gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca ccgagaatgg
    10681 caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc agattgaagt
    10741 gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct tattccaggc
    10801 cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc tggggctggt
    10861 gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata gccagccgaa
    10921 tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca ttattatcga
    10981 agggccatcc acccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac caaactccgc
    11041 agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg tttatagccc
    11101 aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca tcgcggtgac
    11161 cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat cgatgaccga
    11221 tttcctgatg ctggcacttc cttgccatgc acaagtcaat gctgaaaaac tcaagcaaga
    11281 tggtatcggc gcgtgtctgc tgaaaccatt aacacctaca cgcctgttgc ctgccctgac
    11341 ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa gtaagctggc
    11401 aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg gcgcattgct
    11461 ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcgg ttgaacgggc
    11521 gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca tggatggcat
    11581 tcgggcctgc gaactcatcc accaactccc gcatcagcaa caaacgccgg ttatcgcggt
    11641 aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga tgagcgatta
    11701 tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct acaagcctgg
    11761 cagcggtatt tcctctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg tgaacccgaa
    11821 tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg atttagcgcg
    11881 cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag ttgaggaaca
    11941 gctggttgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc atggcagttg
    12001 cggctatagc ggtgtgccac gtatgaagaa tctctgccaa ctgatcgaac aacagctacg
    12061 tagcggtact aaagaagaag atttggaacc ggagctgctg gaactgttgg acgagatgga
    12121 taatgtcgcg cgcgaagcca gcaaaattct cgggtaatgg atgtcttgtc gagtagcaat
    12181 cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta tgccggatgc
    12241 ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta aaaaatgatg
    12301 tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc aaaccggctc
    12361 cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc ttgttatcga
    12421 acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg ccaagcccgt
    12481 gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct acacccagcc
    12541 ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct ttggtcaggc
    12601 gctgattgcc ttcctgccaa atccagtgcg tatcaatagc agtaatttta cccggtgcag
    12661 cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgtta gagtgtgaac
    12721 cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct tgcattgtcc
    12781 agaaatgcgg atccgccagc gggatatcaa cggattgcag ggagagcgta tggcccattt
    12841 gccgccagtc ggtggcgatc atattggttg cagtcggcag acctgtcgcg cgacggaact
    12901 ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct tctgcataag
    12961 ccagcgaacc tttcaggtat ttaccgattt taatcgcttc gttcagcgac caggcaccgt
    13021 taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca atagactcgg
    13081 cctcttcttc cccggccagt acaccgccct tcagtttgaa atcgttgaag ccatattttt
    13141 catatgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct tcatgacgca
    13201 ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagcggc gtggctttgc
    13261 gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt tgaccatcgc
    13321 ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc attgccgctt
    13381 ctatcccggt aactacatga atagtggtac gtaggtcaaa tgtctgcaaa ccgcgcccac
    13441 cagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg tattcaccca
    13501 gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt ttctcgccgc
    13561 cgggaatttc ccctacgcca gtgtgaccag aattatcttt gataatcaca atattacgcg
    13621 taaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga cccgccaccg
    13681 gaatgacttt catatcagta ataacaggac tggattgtgt cgccatctta ttgccccgca
    13741 acaggtttca actcgatacg cttgatatcg cccaccagca ccaggtagct cagtaccgcg
    13801 attaaggcat gaacaccaac ataaatcagc gccccattaa acgagccagt cgtgccaacg
    13861 atataaccaa ttgcgattgg cgtgacgatg ccagaaatgt taccgaacat gttgaacagg
    13921 ccaccggaaa gaccgctgat ctcttttggc gcggtatctg ccattactgc ccaacccagc
    13981 gccccgatgc ccttaccgaa gaaggccagc gccataaagc cgatgatcat ccactcaacg
    14041 ttgacgtagt tgcagaacac catcaccatc gacagcaaca tgcccattac gatcggtgtt
    14101 ttacgcgcaa tgttcagcga tcccgtgcgg cgcatcagcc agtcggaaat aatcccaccc
    14161 agcacaccgc cgataaaacc gcaaaccgcc ggaacggaag ccacaaagcc cgctttcaga
    14221 atcgacatcc cgcgtgcctg caccagataa accgggaacc aggtaataaa gaagtaagtc
    14281 agggcgttga tacagtactg accgatataa acgccgatca tcatccgtga ccctagcagc
    14341 tgtttgatct gcccccactt cacgctgaac ggcactttaa ctttggtgtt ttgctgatcc
    14401 atattgatca gcgcgccacc cgcggcgatg tactccagct ctttcttatt tacccccgga
    14461 tgttgatttg gctcgtggat gactttcaac cagataaagc tgatgacaat ccccagaccg
    14521 cccataaaga agaagacgtg tgaccagccc acttcatgcg tcagccagcc cataatcggc
    14581 gcgaagatca ccgttgcgaa gtattgagcg gagttaaaaa tcgacaccgc cgttcccctt
    14641 tcctgcgccg gaaaccaggc cgcaacaatg cgactgttgc cggggaaaga tggcgcttca
    14701 gcaagcccga ccaggaagcg cagcgtaaac agggcaacga taatgccgaa tccactaaag
    14761 atatcgacga agccttgcag caaggtaaac atcgaccaga taaagatcga ccagaagtag
    14821 acgcgttttg aaccaaaacg gtccagcaac cagccaccag ggatctgccc gataacataa
    14881 gcccatgaga aagcagagaa cacatagccc attcccacgg gatcaaggcc gatatctttg
    14941 gccatttccg aaccggcgat agagagcgta gcgcggtcgc cgtagttgaa ggatgtgacg
    15001 ataaacaaca tcaccactat ccagtaacga gcatttgtgc gtttttccac actgctcgca
    15061 gcctgactta aagaactcat tgttgcactc ctgaaaattc gcgttagcca cgctcactct
    15121 ggactgcgac atcgccagga aatcagaggt gacgtagggt gttttttgcc gtttttatag
    15181 gtcgttcgcc gaatacggcg cgtgtttata tctggcaata gcagtataaa aagcgcgcca
    15241 tagcggctca ccgtgcaaca acacaacatt aatgcgttca atgaggcccg attttggcat
    15301 tagccctgga cggtggaatc cacttcacgg aaatgaaaac aagaacaaga aaggaagggt
    15361 taaaacgaag aaataaagaa gagtatgaaa tggatcgctt gactccaggc aaacgccagt
    15421 aaaaatccgc gctatgaagc agtttttact ggcatttgcc tgaaaagatt cgattcagca
    15481 ccgctaaaac gacatttacc gctcgctgaa catatcagga caacagcgtg ccccactgtt
    15541 cgacccacgg atttgattcc gtttccggtt ccgggttctc actggcgtca atcaacagca
    15601 tttcgccaac ccgctgagcg ctctgttcct gcaacaaggc atcgaactgt ttgccgccat
    15661 tgcagaaatt cacataacta ctgtcgccga gcgcaatcac gccataacgc agattcggct
    15721 ggaagcccag actatctttg attccctgaa agagtggcac aatgctatca ggaaggtcgc
    15781 cctgcccggt cgtggacgta acaaccagaa catacttatc ctgatagggc agccagtcgc
    15841 ttaattcagg atcttcaaat accgttgctt tgtggccctg cgcggtcaga atcgcttccg
    15901 cttcttcggc cactaacagt gaattcccgt acatggtgcc gacaaaaata ccaatttccg
    15961 ccataccgtt ttctccctgg attaggaact tatctctcca tcctgacccg atggcgcact
    16021 gaactcaacc ctttcatttt caggaagcag accgcgccag ccaaattgtg ataacgcctg
    16081 catccaggtg tcgtccaggc ccgcgtgaat agtcagcggc tcgccagtaa aaggatgcgt
    16141 caatgacaac tgactggcat gtagcattaa ccgctggagg ccaaaatgct cagcaccgct
    16201 gcgattctgg cgtaaatcgc catgtttgct atcgccaata atcggatgac gcaaatgggc
    16261 aagatgtcgg cgaagctgat gtttgcgtcc ggttttcggc tccagttcca ccaggccgta
    16321 gcgcgtggtc gggtaacgtc cggtcgctac cggcatttct acggtcgcca gaccgcgata
    16381 atgcgtcact gctggctgcg ggcctttatc ttcgcgggca aatttatcag cgattttgtc
    16441 cagttcttcc accagtggat aatccagcac cgcttcttcc atcaaccagc cgcgcacaat
    16501 cgcatggtaa cgtttctgga tttggtgctg ttcaaactgt tgtgccagca gccgtccggc
    16561 ctcgctggat aatcccatca acaacacacc agaagtgggt cggtccagac gatgagcagt
    16621 aaaaacatgc tggcctatct ggtcacgcac ggtttgcatg accactactt tctcgtcgcg
    16681 atccagccag ctgcggtgaa ccagccagcc ggagggttta tttaccgcaa ccagccattc
    16741 atcctgatag agtatttcca gcattagctc gcatcatccg caaaaagagc atccagtttt
    16801 tccagctcag ccagaataag cgcgcgttgc ggatggtccg tcgccagcgc catttcataa
    16861 tagggtgcaa cggcaaaagc gcccggtaac ggctgtttgt tatctaacaa atcgtgcatt
    16921 cgcgggatca gcacccactg caaccactcc agtggttcca tggtgtccat aaagaacggt
    16981 tgggtactat taaattgatg cggctggggt tcatcgtttc gccagtgctg atgttcacgc
    17041 agtaacgctt caagcgcctg caactggaga cgaacgcggt catgagtggt cataaagcaa
    17101 cctcaataag aaaaactgcc gcgaaggata gcaggaataa aaaaagggag cactgtattc
    17161 acagcgctcc cggttcgttt cgcagcattc cagctacttt tgttgctccc tgctcatcct
    17221 tgacaacttt tcctctggcc ttgcggccaa tcgttcatcc tgaactattg cttcctgctc
    17281 acaccacccc gatgtgatac ttcatcctga agtgtccctg gccttcctga cccaccgaat
    17341 catcctgacc ggttctcatt ctccatcctg gaggtgtcct ttaacgcgtc ctgcgtcatc
    17401 ctcttcgctt catccagaag cctttccctg aaacaccatc ctggtgtgtc ctgcagaagt
    17461 gtcatcatcc tgatgttcac ttcgttgtct gactccctgt cgacgaagat agaatcgtct
    17521 ttttcgcgaa gtcttacaag gcgcttacag acaatgcctt aaggaaaatt tcatatgaaa
    17581 gttaagaatt aacaatcaac attttgattt taaatgaaaa taacgattct tatcttttta
    17641 agtattcgct tatttcctgg cgatctccta cagagtctgt aagagatctc tcacaaccag
    17701 atggggtatt tggattacag aagaggctta agttgattaa ggaattccgc aagatttgag
    17761 gctaaatgcg tccgtttacg tgttcccagc gtctctttgc acacttcacc ggagagatta
    17821 cacaccgaga tgacctctaa ctcttcttcc agcgtcgcga taaatagcgt gggcggcagt
    17881 tttagccttt tctgagtcac cagatgacca atcagatttt cctgcacccg acggaagtcg
    17941 tcttcactcc aggtttgcag caatgtcagt ttgatatcgc caaactgcgc gtgcatatcc
    18001 ccggcaaact gagtcgtata aaaggtatga attgtgggtt gtatcacaat atcaaaagcg
    18061 cgttcaaccg cgtttacatt ttgttccccg gtaaacggct ggggttgcca gtatacggca
    18121 tcttcggtag ttgaaataat gcacggtgaa ggaacaccgt acagttcctc acttaacggc
    18181 cagcttttat gctcttcatg ccacgcatcg cagtagcgtg cagtaaaatc tttcagggct
    18241 tgtgcggtca aatcgtccac taatttctct cttcacttaa accagataca cttgtcgttt
    18301 agtttatctg gtttatgacg gtgaaacatg tcttcttatg caaaccatca ggcacttgcg
    18361 ggcctgactc ttggaaaatc aaccgattac cgggatacct atgacgccag cctactgcaa
    18421 ggcgttccac gcagcctgaa tcgcgacccg ctgggtctga aagcggataa cctgcctttt
    18481 cacggtacgg atatctggac gctgtatgaa ctttcctggc tgaatgcgaa aggtttgccg
    18541 caggtcgctg tcggtcatgt tgaacttgat tacaccagcg taaatctgat tgagtcgaag
    18601 agttttaagc tctatctcaa cagttttaac cagacgcgtt ttaataactg ggatgaggtg
    18661 cgccagacgc tggagcgcga cttaagcact tgcgctcagg gtaagattag cgtggcgtta
    18721 tatcgtcttg atgaactgga aggtcagccg ataggtcatt ttaatggcac ttgcattgat
    18781 gaccaggata tcactatcga taactatgaa ttcactactg actatctgga gaatgccacc
    18841 tgtggtgaaa aagtagtgga agagacgctt gtcagccacc tgctgaaatc aaactgcctg
    18901 atcacccatc aaccagattg gggttcgatc caaattcagt atcgtggacg ccaaattgac
    18961 agagaaaaac tgctgcgtta cctggtctca ttccgtcatc acaacgagtt ccacgaacag
    19021 tgcgtggaac gcatctttaa tgacctgtta cgcttctgcc agccagaaaa attgagcgtt
    19081 tacgcacgtt atacccgtcg tggcggtctg gacattaacc cgtggcgcag taatagcgat
    19141 tttgtcccat cgaccacaag actggttcgg caataaattt tttctcaatt ttgcgtgctg
    19201 gattcacgca gaaggttgtg aaaggtcatc aggcagggct attgtaatca aagggaatga
    19261 cgatattcgt cccataagga gtttttcttg attacacata ttagcccgct tggctccatg
    19321 gatatgttgt cgcagctgga agtggatatg cttaaacgca ccgccagcag cgacctctat
    19381 caactgtttc gcaactgttc acttgccgta ctgaactccg gtagtttgac cgataacagc
    19441 aaagaattgc tgtctcgttt tgaaaatttc gatattaacg tcttgcgccg tgaacgcggc
    19501 gtaaagctgg aactgattaa tcccccggaa gaggcttttg tcgatgggcg aattattcgc
    19561 gctttgcagg ccaacttgtt cgcggtcctg cgtgacattc tcttcgttta cgggcaaatc
    19621 cataacaccg ttcgttttcc caacctgaat ctcgacaact ccgtccacat cactaacctg
    19681 gtcttttcca tcttgcgtaa cgctcgcgcg ctgcatgtgg gtgaagcgcc aaatatggtg
    19741 gtctgctggg gcggtcactc aattaacgaa aacgagtatt tgtatgcccg tcgcgtcgga
    19801 aaccagctgg gcctgcgtga gctgaatatc tgcaccggct gtggtccggg agcgatggaa
    19861 gcgccgatga aaggtgctgc ggtcggacac gcgcagcagc gttacaaaga cagtcgtttt
    19921 attggtatga cagagccgtc gattatcgcc gctgaaccgc ctaacccgct ggtcaacgaa
    19981 ttgatcatca tgccagatat cgaaaaacgt ctggaagcgt ttgtccgtat cgctcacggt
    20041 atcattatct tccctggcgg tgtgggtacg gcagaagagt tgttgtattt gctgggaatt
    20101 ttaatgaacc cggccaacaa agatcaggtt ttaccattga tcctcaccgg cccgaaagag
    20161 agcgccgact acttccgcgt actggacgag tttgtcgtac atacgctggg cgaaaacgcg
    20221 cgccgccatt accgcatcat cattgatgac gccgctgaag tcgctcgtca gatgaaaaaa
    20281 tcgatgccgc tggtgaaaga aaatcgccgc gacactggtg acgcttacag ctttaactgg
    20341 tcaatgcgca ttgcaccaga tttgcaaatg ccgtttgagc cgtctcacga gaatatggct
    20401 aatctgaagc tttacccgga tcaacctgtt gaagtgctgg ctgccgacct gcgccgtgcg
    20461 ttctccggta ttgtggcggg taacgtaaaa gaagtcggta ttcgcgccat tgaagagttt
    20521 ggtccttaca aaatcaacgg cgataaagag attatgcgtc gtatggacga cctgctacag
    20581 ggttttgttg cccagcatcg tatgaagttg ccaggctcag cctacatccc ttgctacgaa
    20641 atctgcacgt aatctcccct cccccggtga cgtttgttgc cgggttttct cgtttttggt
    20701 cacttactca tcaactcatt tcatttgtta tatgaatgtt tcttaccacc ctcacgcgac
    20761 aaatatcatc acagttaata tgtcatacaa tttatattgc aacgcaaacg tttccctatt
    20821 ttcataaacc gtattttatc gctatgaaaa agaaattatc gccatgatta actaaaagta
    20881 ttgatttttt cagttcaacc tacatatatt gcgcgccccg gaagaagtca gatgtcgttt
    20941 aatgggcaaa tattgccctt aaattctctt ttacttttaa tttacagagt aaagcgttgg
    21001 gataatctat cttccaagta gattattgta tttgggatca agatcactga tagatacata
    21061 acttgtgtgt atctttccgc cctcaaatta ttacggcggt aaatgattaa gccatcgccg
    21121 atagacagat ttcattttta cggtcaggca ccttcccggg ctgaactggc taaaagctga
    21181 attatttgca ttcctccagg agaaatagat ggaaacgact caaaccagca cgattgcgtc
    21241 gaaagactct cgtagtgcct ggcgcaagac agacaccatg tggatgctgg gcctttacgg
    21301 cacggcaatc ggcgcgggcg tgctgttcct gccaatcaac gccggtgttg gcggtatgat
    21361 cccgctgatc atcatggcta tccttgcgtt cccgatgacg ttttttgctc accgcggcct
    21421 gactcgcttc gtactgtctg gtaaaaaccc gggcgaagac atcaccgagg ttgtagaaga
    21481 acactttggt attggcgcag gtaaactgat taccctgctc tacttcttcg ctatctaccc
    21541 gatcctgctg gtttatagcg tggcaatcac caataccgtt gaaagcttca tgtctcacca
    21601 gctgggtatg acgccaccgc cgcgtgcgat tctgtcgctg atcctgatcg tgggtatgat
    21661 gaccatcgtt cgcttcggtg agcagatgat cgttaaagcg atgagtattc tggtattccc
    21721 gtttgttggc gtactgatgc tgctggctct gtacctgatc ccgcagtgga acggcgctgc
    21781 actggaaacg ctgtctctgg acactgcatc tgcaaccgga aacggtctgt ggatgaccct
    21841 gtggctggca attccggtaa tggtgttctc gttcaaccac tctccgatca tctcttcttt
    21901 cgccgttgcg aagcgtgaag agtacggcga tatggcagaa cagaaatgct cgaagatcct
    21961 ggcattcgca cacatcatga tggtgctgac cgtaatgttc ttcgtcttca gctgtgtact
    22021 gagcctgact ccggcagacc tggctgcggc taaagagcag aacatctcga ttctgtctta
    22081 cctggctaac cactttaacg caccggttat cgcgtggatg gctccgatta tcgcgattat
    22141 cgctatcacc aaatccttcc tcggtcacta cctgggcgca cgtgaaggct tcaacggtat
    22201 ggtgattaaa tctctgcgtg gtaaaggtaa gtctatcgaa atcaacaagc tgaaccgtat
    22261 cactgcgctg ttcatgctgg taacgacctg gattgttgcc accctgaacc cgagcatcct
    22321 gggtatgatt gaaaccctgg gcggtccaat catcgcgatg atcctgttcc tgatgccgat
    22381 gtacgcaatt cagaaagtac cggcaatgcg taagtacagc ggtcacatca gcaacgtatt
    22441 cgttgtcgtg atgggtctga ttgcaatctc cgcaatcttc tactctctgt tcagctaagt
    22501 cctttcgcgc cgctttcggg cggcgcttcc tccgttttaa cgcgatgtat ttcctatgat
    22561 tagcgtattc gatattttca aaatcggcat tggcccttcc agttctcata ccgttggacc
    22621 aatgaaagcg ggtaaacaat ttaccgacga tctgattgcc cgtaacctgc ttaaagacgt
    22681 gacccgcgtg gtggttgacg tgtacggctc gctctctctg accggtaaag gccaccacac
    22741 tgatatcgcc attattatgg gcctggcggg taacctgccg gataccgtgg atatcgattc
    22801 catccctggt tttattcagg atgtgaatac tcatggtcgc ctgatgctgg caaacggtca
    22861 gcatgaagtg gagttcccgg ttgatcagtg catgaacttc catgcagaca acctttctct
    22921 gcatgaaaac ggtatgcgca ttaccgcgct ggcgggcgat aaagtcgttt acagccagac
    22981 ttactactct attggcggtg gctttatcgt tgatgaagag cattttggcc agcagaatag
    23041 cgcaccggtt gaagttcctt atccgtacag ttcagcagcc gatctgcaaa aacattgtca
    23101 ggaaaccggg ctgtcactct ctggcctgat gatgaaaaat gagctggcgc tgcacagcaa
    23161 agaagagctg gaacagcacc tggcgaacgt ctgggaagtc atgcgcggcg gtattgagcg
    23221 cggtatttct accgaaggcg tgttgcctgg caaactgcgc gtaccacgcc gtgctgcggc
    23281 actacgccgg atgctggtca gccaggataa aaccaccact gacccgatgg cggttgttga
    23341 ctggatcaac atgtttgcac tggcagtgaa cgaagagaac gccgctggcg gtcgcgtggt
    23401 gactgcgccg actaacggtg cgtgcgggat tatcccggct gtgctggcgt actacgataa
    23461 gtttatccgc gaagtgaacg ctaactcact ggctcgttac ctgctggtag ccagcgccat
    23521 tggttctctt tataagatga acgcgtcaat ttctggtgct gaagtcggct gccagggtga
    23581 agttggtgtg gcgtgctcaa tggcggcggc tggtctggca gaactgttag gtgcaagccc
    23641 ggcgcaggtg tgcatcgcgg cggaaatcgc catggagcac aacctcggtc tgacgtgtga
    23701 cccggtcgcc ggacaggtcc aggtgccatg catcgagcgt aacgccattg cggcagtaaa
    23761 agcggtgaac gccgcacgta tggcgctacg ccgtaccagc gagccgcgcg tctgcctcga
    23821 taaagttatc gaaaccatgt acgaaacagg taaagatatg aacgccaagt accgcgaaac
    23881 ctctcgcggc ggcctggcaa tgaagatcgt tgcctgcgat taatcgctct ccagaggcct
    23941 cgttttgcga ggcctcttcc cgatttctca tccagccgta gcctgttccg gcatcgaatg
    24001 ttacccttat cgcctgatct ttaagggggt tatcgtggct gttcatttgc ttattgtcga
    24061 tgcactgaat cttattcgtc gcattcatgc cgttcaggga tcgccctgtg tcgaaacctg
    24121 ccagcatgcg ctcgatcagc tcattatgca cagccagcca acccacgcgg tcgccgtttt
    24181 tgatgatgaa aaccgcagta gcggctggcg tcatcagcgt ttaccagatt acaaagcggg
    24241 tcgaccgcca atgccggaag agttgcacga cgagatgcct gcattacgcg ccgcctttga
    24301 gcaacgcggc gtcccgtgct ggtcaaccag cggcaacgaa gccgatgact tagccgccac
    24361 gctggcggtc aaagtgacac aggccgggca tcaggcaacg attgtttcga cagataaagg
    24421 ctactgtcag ttactttcac cgacattacg tattcgtgat tacttccaga aacgttggct
    24481 ggatgcgcca tttatcgata aagaatttgg cgttcaaccg cagcagttgc ccgattactg
    24541 gggacttgcg gggatcagca gttcaaaggt accgggtgtt gcgggaatcg gaccaaaaag
    24601 cgccacgcag ctgctggtcg agtttcagag tctggaaggg atatatgaga atctggatgc
    24661 ggttgccgaa aagtggcgca aaaaattaga aacccataaa gagatggcgt ttctgtgccg
    24721 cgatattgcc cgcttacaaa ccgatttgca tatcgacggc aatttacagc aattgcggtt
    24781 ggtacggtaa cggcgagccg gatacgccgc aaacgtcgta tccggcatta tcacatcagc
    24841 gcatttacca ggcggtatgg taaagctcta caatatcctc aagcgttgct tcacgcgggt
    24901 tgccaccggt acaaacatca tccagtgccg cctgcgccag tgccggaatg tcttccttgc
    24961 gtacaccaac atcacgcaaa tgtggcggaa taccgacatc acggttgaga gcaaacaccg
    25021 cttcaacagc ggcattacgc gcctcttcca ggctcatacc ttccactttc acgcccataa
    25081 cgcgcgcgat atcgcggtac ttctcaccgg taaagtcagc gttataacgc atgacatgcg
    25141 gtaacaggat ggcgttcgca acaccgtgtg gagtgttata aaacgcgccc agtggatgcg
    25201 ccataccatg caccaaccct aacccaacat tcgagaagcc catacccgca acatactgcc
    25261 cgagcgccat ttcttctccg gcatccttat caccagcaac cgatcctcgc agcgccccag
    25321 caatgatttc aatcgcttta atgtgcagtg catcggttag cgcccacgcg ccacgggtaa
    25381 tatacccctc aatagcatga gtgagcgcat cgacacccgt cgcagctttc agcgctggag
    25441 gcataccatc catcatgtca gcgtcaataa acgccacctg cgggatatca tgcggatcaa
    25501 cgcaaacaaa cttgcgccgt ttctcttcgt cagtgatcac gtagttaatg gtcacttctg
    25561 ccgcagtacc tgctgtggta ggaattgcca gaatcggtac actgggttta ttggtcgggg
    25621 aaagcccttc caggctacgc acatcggcaa actccgggtt gttgctgata atgccaatcg
    25681 ctttacaagt atcctgtgga gaaccaccac caatagcgat caggtaatcc gcgccgctat
    25741 tctggaatac accgagccct tctttgacga cagtaattgt tgggttgggc actacgccgt
    25801 cgtaaatcgc ccatgccagc cctgcagcat ccatcttatc ggtcactttc gccaccacgc
    25861 cgcattgcac cagcgtttta tcggtgacga tcagcgcctt ctgataacca cggcgtttca
    25921 cctcatcggt taaagcccca acagcacccc gaccaaacca tgccgtttcg ttcagaatca
    25981 ttctgttagc catcatcctt ctccttgttg ctttacgaaa ttactcttca atccgtaacc
    26041 cataggtttt gaatttctcc agcactacgg caatctcttc atcgctcagc actggcaccg
    26101 ggtccgtaat cgccagggtc gtcaggtaaa gttgcgccag cacttcaact tcatgcgcca
    26161 gccataacgc tttttccaga ttcacctcac aagcgataag cccatgatgt tgtaacaaag
    26221 ttgccttacg atttttgaga gccagcgcaa catgttcaga aagttcgcgt gttccaaagg
    26281 tcgcataagg cgcgcaagga atagaattac cgccagccgc cgcaatcatg tagtgaatag
    26341 cggggatcgg tcggttaaga atggaaactg ctgtgcaatg aacggcatga ttgtgaacaa
    26401 ccgcgttggc atccggtctg ctttgatagg ccgccatatg gaaacgccat tcgcttgagg
    26461 ggagctttcc ttcctcatgt ttaccgttgc catcaataaa gacaatatgc gactccgtca
    26521 gtttttcata tggaatgcct gtaggcgtaa tcagcatccc atcctgataa cgtacactga
    26581 cgttccccgc tgtcccctgg ttcagtccca ggcgggtcat ttccaggcaa gtgtcaataa
    26641 tctgacgagc aagtttattt cgttccatta gctacctctc tctgattcaa aacagggcaa
    26701 taatgttgtt cctttcacac tattgaatta gccgtttaat tacccaccat cttcttcctg
    26761 attaaccaga aagaaattca caagcttata ttttgtgacc tggttcaact aatcacagta
    26821 aataactgca agttctcttt ttataacccc attaaaaatg accgctctta aaaatattta
    26881 tcaaaacggt catttttcta ttcactcaag ccaggaatgc ccgttttcgg cataaacaat
    26941 taatacggtc atctggtttg tgttttttat gatttatttt ctgaaacggg catgaaattt
    27001 cgattattaa agtgatggta gtcacataaa gtcaccttcc agctaataag tgtgaccgcc
    27061 gtcatattac agagcgtttt ttatttgaaa atgaatccat gagttcattt cagacaggca
    27121 aatattcact gatatgaagc ccgaactcgc tggttttgca cttttgaaaa cataaccgat
    27181 tacgtgctta agcttctgaa cctaagagga tgctatggga aacacatcaa tacaaacgca
    27241 gagttaccgt gcggtagata aagatgcagg gcaaagcaga agttacatta ttccattcgc
    27301 gctgctgtgc tcactgtttt ttctttgggc ggtagccaat aaccttaacg acattttatt
    27361 acctcaattc cagcaggctt ttacgctgac aaatttccag gctggcctga tccaatcggc
    27421 cttttacttt ggttatttca ttatcccaat ccctgctggg atattgatga aaaaactcag
    27481 ttataaagca gggattatta ccggattatt tttatatgcc ttgggcgctg cattattctg
    27541 gcccgctgca gaaataatga actatacctt atttttagtt ggcctattta ttattgcagc
    27601 cggattaggt tgtctggaaa ctgccgcaaa cccttttgtt acggtattag ggccggaaag
    27661 cagtggtcac ttccgcttaa atcttgcgca aacatttaac tcgtttggcg caattatcgc
    27721 ggttgtcttt gggcaaagtc ttattttgtc taacgtgcca catcaatcgc aagacgttct
    27781 cgataaaatg tctccagagc aattgagtgc gtataaacac agcctggtat tatcggtaca
    27841 gacaccttat atgatcatcg tggctatcgt gttactggtc gccctgctga tcatgctgac
    27901 gaaattcccg gcattgcaga gtgataatca cagtgacgcc aaacaaggat cgttctccgc
    27961 atcgctttct cgtctggcgc gtattcgtca ctggcgctgg gcggtattag cgcaattctg
    28021 ctatgtcggc gcacaaacgg cctgctggag ctatttgatt cgctacgctg tagaagaaat
    28081 tccaggtatg actgcaggct ttgccgctaa ctatttaacc ggaaccatgg tgtgcttctt
    28141 tattggtcgt ttcaccggta cctggctcat cagtcgcttc gcaccacaca aagtcctggc
    28201 cgcctacgca ttaatcgcta tggcactgtg cctgatctca gccttcgctg gcggtcatgt
    28261 gggcttaata gccctgactt tatgcagcgc ctttatgtcg attcagtacc caacaatctt
    28321 ctcgctgggc attaagaatc tcggccagga caccaaatac ggttcgtcct tcatcgttat
    28381 gaccatcatt ggcggcggta ttgtcactcc ggtcatgggt tttgtcagtg acgcggcggg
    28441 caacatcccc actgctgaac tgatccccgc actctgcttc gcggtcatct ttatctttgc
    28501 ccgtttccgt tctcaaacgg caactaactg aacatatttt ccgaataaag tgaggaatct
    28561 gtaatgaaaa aaatcagctt accgaaaatt ggtatccgcc cggttattga cggtcgtcgc
    28621 atgggtgttc gtgagtcgct tgaagaacaa acaatgaata tggcgaaagc tacggccgca
    28681 ctgctgaccg agaaactgcg ccatgcctgc ggagctgccg tcgagtgtgt catttccgat
    28741 acctgtatcg cgggtatggc tgaagccgct gcttgcgaag aaaaattcag cagccagaat
    28801 gtaggcctca ccattacggt aacgccttgc tggtgctatg gcagtgaaac catcgacatg
    28861 gatccaaccc gcccgaaagc tatttggggc tttaacggca ctgaacgtcc cggcgctgtt
    28921 tacctggcag cggctctggc agctcacagc cagaaaggca tcccggcatt ctccatttac
    28981 ggtcatgacg ttcaggatgc cgatgacaca tcgattcctg ccgatgttga agaaaaactg
    29041 ctgcgctttg cccgcgccgg tttggccgtc gccagcatga aaggtaaaag ctatctgtcg
    29101 ctgggcggcg tttcgatggg tatcgccggt tctattgttg atcacaactt ctttgaatcc
    29161 tggctgggaa tgaaagtcca agcggtggat atgaccgaac tgcgtcgccg tatcgatcag
    29221 aagatttacg acgaagccga actggaaatg gcactgacct gggctgataa aaacttccgc
    29281 tatggcgaag atgaaaataa caaacagtat caacgcaatg ccgagcaaag ccgcgcagtt
    29341 ctgcgcgaaa gtttactgat ggcgatgtgt atccgcgaca tgatgcaagg caacagcaaa
    29401 ctggccgata ttggtcgcgt ggaagaatca cttggctaca acgccatcgc tgcgggcttc
    29461 caggggcaac gtcactggac cgatcaatat cccaatggtg acaccgccga agcgatcctc
    29521 aacagttcat ttgactggaa tggcgtgcgc gaaccctttg tcgtggcgac cgaaaacgac
    29581 agtcttaacg gcgtggcaat gctaatgggt caccagctca ccggcaccgc tcaggtattt
    29641 gccgatgtgc gtacctactg gtcaccagaa gcaattgagc gtgtaacggg gcataaactc
    29701 gatggactgg cagaacacgg catcatccat ttgatcaact caggttctgc tgcgctggac
    29761 ggttcctgta aacaacgcga cagcgaaggt aacccgacga tgaagccaca ctgggaaatc
    29821 tctcagcaag aggctgacgc ttgcctcgcc gctaccgaat ggtgcccggc gatccacgaa
    29881 tacttccgtg gcggcggtta ctcttcccgc ttccttaccg aaggcggcgt cccgttcacc
    29941 atgactcgtg tcaacatcat caaaggcctg ggaccggtac tgcaaatcgc ggaaggctgg
    30001 agcgtggaat tgccgaagga tgtgcatgac atcctcaaca aacgcaccaa ctcaacctgg
    30061 ccaaccacct ggtttgcacc gcgcctcacc ggtaaagggc cgtttacgga tgtgtactcg
    30121 gtaatggcga actggggcgc taaccatggg gttctgacca tcggccacgt tggcgcagac
    30181 tttatcactc tcgcctccat gctgcgtatc ccggtatgta tgcacaacgt tgaagagacc
    30241 aaagtgtatc gtccttctgc ctgggctgcg cacggcatgg atattgaagg ccaggattac
    30301 cgcgcttgcc agaactacgg cccgttgtac aagcgttaat acattactcc ctgatgtgat
    30361 gcccggtcgc tccggctacc gggcctgaac aagcaagagt ggttagccgg ataagcaatg
    30421 ttatccggct atattgcagg agcgattatg aaacaagaag ttatcctggt actcgactgt
    30481 ggcgcgacca atgtcagggc catcgcggtt aatcggcagg gaaaaattgt tgcccgcgcc
    30541 tcaacgccta atgccagcga tatcgcgatg gaaaacaaca cctggcacca gtggtcttta
    30601 gacgccattt tgcaacgctt tgctgattgc tgtcggcaaa tcaatagtga actgactgaa
    30661 tgccacatcc gcggtatcgc cgtcaccacc tttggtgtgg atggcgctct ggtagataag
    30721 caaggcaatc tgctctatcc gatcattagc tggaaatgtc cgcgaacagc agcggttatg
    30781 gacaatattg aacagttgat ctccgcacag cggttgcagg ctatttctgg cgtcggagcc
    30841 tttagtttca atacgttata taagttggtg tggttgaaag aaaatcatcc acaactgctg
    30901 gaacgcgcgc acgcctggct ctttatttcg tcgctgatta accaccgttt aaccggcgaa
    30961 ttcactactg atatcacgat ggccggaacc agccagatgc tggatatcca gcaacgcgat
    31021 ttcagtccgc aaattttaca agccactggt attccacgcc gactcttccc tcgtctggtg
    31081 gaagcgggtg aacagattgg tacgctacag aacagcgccg cagcaatgct cggcttaccc
    31141 gttggcatac cggtgatttc cgcaggtcac gatacccagt tcgccctttt tggcgctggt
    31201 gccgaccaaa atgaacccgt gctctcttcc ggtacatggg aaattttaat ggtccgtagc
    31261 gcccaggttg atacttcgct gttaagtcag tacgccggtt ccacctgcga actggatagc
    31321 caggcagggt tgtataaccc aggtatgcaa tggctggcat ccggcgtgct ggaatgggtg
    31381 agaaaactgt tctggacggc tgaaacaccc tggcaaatgt tgattgaaga agctcgtctg
    31441 atcgcgcctg gcgcggatgg cgtaaaaatg cagtgtgatt tattgtcgtg tcagaacgct
    31501 ggctggcaag gagtgacact taataccacg cgggggcatt tctatcgcgc ggcgctggaa
    31561 gggttaactg cgcaattaca gcgcaatcta cagatgctgg aaaaaatcgg gcactttaag
    31621 gcctctgaat tattgttagt cggtggagga agtcgcaaca cattgtggaa tcagattaaa
    31681 gccaatatgc ttgatattcc ggtaaaagtt ctcgacgacg ccgaaacgac cgtcgcagga
    31741 gctgcgctgt tcggttggta tggcgtaggg gaatttaaca gccctgaaga agcccgcgca
    31801 cagattcatt atcagtaccg ttatttctac ccgcaaactg aacctgaatt tatagaggaa
    31861 gtgtgaaatg ctgaaaacaa tttcgccgtt aatttctccc gaactattga aagtgctggc
    31921 agagatggga catggagatg aaattatttt ttccgatgct cactttcccg cccattcgat
    31981 gggaccgcag gtgatccgcg ctgatggcct gttggtgagc gacttgctcc aggcgattat
    32041 cccgttattt gaactggaca gttatgcacc gccgctggtg atgatggcgg cggtagaagg
    32101 tgacactctc gatcctgaag tagaacgacg ttaccgtaat gcgctttcac tacaagcccc
    32161 gtgtcctgac atcatccgca tcaatcgttt tgcgttttat gaacgggcgc aaaaagcctt
    32221 tgcgatcgtt atcacaggcg aacgagcgaa gtacgggaat attcttttaa aaaaaggggt
    32281 aacaccgtaa tctcataccg gtacgcccgc atgacgcggg cggttatcga atgatggggt
    32341 gaaaaatatg aaagcggcac gccagcaagc gatagtcgac ctgctgctga accataccag
    32401 cctgaccacg gaagctctct ctgaacagct aaaggtcagt aaagaaacca ttcgtcgcga
    32461 tctcaatgaa ttacagacgc agggtaaaat tctgcgcaat catggacgcg ctaaatatat
    32521 ccaccgtcaa aatcaagaca gtggcgatcc ctttcacatc aggctgaaaa gccattatgc
    32581 gcataaagca gatatcgcgc gcgaggcgct cgcgtggatt gaagaaggga tggtgatagc
    32641 cttagacgcc agttcaactt gctggtatct ggcacgccag ttgcctgaca tcaacattca
    32701 ggtcttcacc aatagccatc cgatttgcca tgaactcggt aaacgcgaac gcattcaact
    32761 gatcagttcc ggcggcacac ttgagcgcaa atatggctgt tacgtcaatc cctcgctgat
    32821 ttcccaactt aaatcgctgg aaatcgatct gtttattttt tcttgtgaag ggatcgatag
    32881 cagcggcgca ctgtgggact ccaatgcgat caacgctgat tacaaatcga tgctattaaa
    32941 acgtgccgcg caatcgttgt tattgattga taaaagtaaa tttaatcgtt caggggaagc
    33001 ccgcatcggg catctggatg aggtaacgca cattatttct gatgagcgcc aggttgcaac
    33061 ttctttggta acagcctgac ggaaagggta gcaggccgaa gacgacgccc cggccttgcc
    33121 tgttatcgct cgtcgcgacg accacccacc gcagcccaga tgcggcggac gtgcaccgtc
    33181 acttcttcgc gatcgtgata caactgccgc gcctgaatct gagcatttat gccatgttca
    33241 tcaagctgtg cctgaatata cgccagattg tgtgatactt cttcgtagcg ttttttcatc
    33301 ggcagtttga gattgaaaat ggtttcacgg caccagccat taaccagcca ctgcgccatc
    33361 aatgccgcaa ctttagccgg tttttcaacc atatcgcata ccatccagga gatattgctg
    33421 cgtgtcggac ggaatttgaa accgtcttcc cgcagccacg tcacctgccc ggtatccatc
    33481 agactttgcg ccatcgggcc gttgtcgacg gaataaaccc acatgttgcg cttcaccagt
    33541 tggtaggtcc agccgccagg gcaagcgcct aaatccaccg cccacatccc gttcgccagg
    33601 cgttcatccc actcatccgc aggaataaac acatgaaatg cctcttccag tttgagcgta
    33661 gaacgactcg gcgcatctgc cggaaatttc aggcgcggaa tgcccatata gaacggcgaa
    33721 ttattgttgc tgtatgagta accggtatag cagcagcctg gtgcaatgaa gaatacatgc
    33781 actaccggac gcttcggcgt ttcatagttc gccagcaccc cggcatcgcg caaggcagcg
    33841 cgtagcggca cggtgaattt acggcagaat ttcagcaact ctttgctttc gttggtatcg
    33901 gcaacttcaa cacgcagttc accgcccttc tctactacgc cctgtaacat gccgacaatg
    33961 ggggtaatac gatcttctgg cggcaaatgc tgcaggagtt cccccaccac aaaccactgg
    34021 cgggcaaaaa ttaatgaact gaacggcagc tcacggatta acttatcgcc atcatcaggt
    34081 tgataacatt cataaatgac ataacccgca ttctctttca cgcgggcaaa accgaaaatt
    34141 tcccgctggc cggctttatc ggtaatttct gcggcgcact ctttttcaaa gcccggacgg
    34201 cacagcaata caaccttatt catgacttac gcccttgcgc tttaaacgga tagcaccaac
    34261 taacattaac gcccagcccg caaggaagct cacgccgcca accggagtga caaacgccca
    34321 caaacgcaga tgggacagtg ccaggcaata aaggctgccg ctgaacagca ccgtgcctaa
    34381 cgcgaggaaa acgctactcc agtaaaacca gatactgatg cgacgctgca ttgccaccgc
    34441 cagacctaag atcgccagcg tatgaaacgc ctggtattcg aggccggtct ggatccagcc
    34501 catctcaacg gcccccatgg ttttacttaa cacatgcgcg ccaaaagcgc ccagagccac
    34561 aaaaatgaag ccgctaatgg cggcgaaaat cagcataaaa cggctggtca tggtcgtacc
    34621 ctacgtaaat tattgttcat aacgaaagcg gaatttttct tgttcagcag cggctttcgc
    34681 caggatccat tggcgaaagg cggctatttt acccagttct gcctgactgt catgacaaac
    34741 cagataaaaa gcatttttac tgaccagaac atcattaaac gggcaaacaa gacgtccggc
    34801 ctcgatttca gattgcgcca tcacgttatt tgccagcgcc actccctgcc cgtggatagc
    34861 cgcttgcagc accatggcgc tatggctaaa aattggccct tgctgaacgt tgatatgatt
    34921 taaccccaac tgtcgggtat atgtctgcca gtcacggcgc gaagcatcat gtaataacgt
    34981 atgtttagcc agatcttccg gtgtcttcaa gggtttttcg ccagtcagca gtagcggcga
    35041 acacaccggc aataaatatt cggcgtacag tttttccacc cgtagccccg gccagttgcc
    35101 ccgaccataa aatatcgcca catcaacatc atccgccagc ttatcttcct gacgatcaac
    35161 cgcctggatt cgaacgtcaa ttcccggata agctgaatta aagctggaaa gtcgcggaac
    35221 caaccaatga atggcgaaac tggggagtaa actgaccgtc aacgccccct tggcgctacg
    35281 ggcctggagt ttacgcgtcg cttcggttaa ttgcgaaaat atctctttga tatcgaggaa
    35341 atagctttgc ccttcctcgg tcagcaggag tgaacgatta cggcggcgga acagttttag
    35401 ccccaaaaaa tcctcaagag acttgatttg atgacttact gcggcctggg tcacaaaaag
    35461 ctcttctgct gcgcgagtga aacttaaatg gcgtgctgcg gcatcaaaaa ctcgtaaggc
    35521 atttagcggt ggtaatcgtt tagacatggc tattaaactt tgatgttaaa tgaatttaac
    35581 aattagatca cactatgtaa cctattagtt tttttaatct gagccattat aaattgtccg
    35641 ttgagcttct accagcaaat acctatagtg gcggcacttc ctgagccgga acgaaaagtt
    35701 ttatcggaat gcgtgttctg gtgaactttt ggcttacggt tgtgatgttg tgttgttgtg
    35761 tttgcaattg gtctgcgatt cagaccatgg tagcaaagct accttttttc acttcctgta
    35821 catttaccct gtctgtccat agtgattaat gtagcaccgc ctaattgcgg tgcttttttt
    35881 taacttgcga tcgcgaatta ctgatccagt tcgaccatct ctttcacgtc agtacggttg
    35941 atctgctgtt tgttgccatt agcgtcttta tacgaaatca taccggtatc gttatcagtc
    36001 tgtggtttgc cgtcagagac gatggtacgt ccgtcattgg tgtgcatcac atagttcgaa
    36061 ccggaacagg cgctcagggc aaaagtcagc atacaggcag aaataattgc ggcagtcttt
    36121 ttcatcatct tctccttaaa gcgattaata aatcttaata gtattgatta acaggctaaa
    36181 attaacgcct aacactattc agcatatgtt acttacgcgg cttcgccagg atatccagat
    36241 aattctgatg gttagcactc tccttgtatc aaagtgaatt ttgcgtcacg atcggtgcat
    36301 caagccgagg agtaccatga acgtttttaa tcccgcgcag tttcgcgccc agtttcccgc
    36361 actacaggat gcgggcgtct atctcgacag cgccgcgacc gcgcttaaac ctgaagccgt
    36421 ggttgaagcc actcgacagt tttacagcct gagcgccgga aacgtccatc gcagccagtt
    36481 tgccgaagcc caacgcctga ccgcgcgtta tgaagctgcg cgggaaaaag tggcacaatt
    36541 actgaatgca tcggatgata aaactatcgt ctggacgcgc ggcaccactg aatccatcaa
    36601 catggtggca caatgctatg cgcgtccgcg tctgcaaccg ggcgatgaga ttattgccag
    36661 cgtggcagaa caccacgcca acctcgtccc ctggctgatg gtcgcccaac aaactggagc
    36721 caaagtggtg aaattgccgc ttaatgcgca gcgactgccg gatgtcgatt tgttgccaga
    36781 actgattact ccccgtagtc ggattctggc gttgggtcag atgtcgaacg tgactggcgg
    36841 ttgcccggat ctggcgcgag cgattacctt tgctcattca gccgggatgg tggtgatggt
    36901 tgatggtgct cagggggcgg tgcatttccc cgcagatgtt cagcaactgg atatcgattt
    36961 ctatgctttc tctggtcaca aactgtatgg cccgacgggt atcggcgtgc tgtatggcaa
    37021 atcagaactg ctggaagcga tgtcgccctg gctgggtggc ggcaaaatga ttcacgaagt
    37081 gagttttgac ggcttcacga ctcaatctgc gccgtggaaa ctggaagcag gaacgccaaa
    37141 tgtggctggt gtcataggat taagcgcggc gctggaatgg ctggcagatt acgatatcaa
    37201 ccaggcagaa agttggagtc gcagcctggc gacgctggcg gaagatgcgc tggcaaaacg
    37261 tccaggcttt cgttcattcc gctgccagga ttccagcctg ctggcctttg attttgctgg
    37321 cgttcatcac agcgatatgg tgacgctgct ggcggagtac ggtattgccc tgcgcgccgg
    37381 gcagcattgc gctcagccgc tactggcaga attaggcgta accggcacac tgcgcgcctc
    37441 ttttgcgcca tataatacaa agagtgatgt ggatgcgctg gtgaatgccg ttgaccgcgc
    37501 gctggaatta ttggtggatt aatgacgaac ccgcaattcg ccggacatcc gttcggcaca
    37561 accgtaaccg cagaaacgtt acgcaatacc ttcgcaccgt tgacgcaatg ggaagataaa
    37621 tatcgccagt tgatcatgct ggggaaacag cttccggctt tgccagacga gttaaaagcg
    37681 caggctaaag agattgccgg atgcgaaaac cgcgtctggc tgggatatac cgtggctgaa
    37741 aacggtaaaa tgcacttctt tggcgacagc gaaggacgta tagtgcgcgg cctgctggcg
    37801 gtgctgttga ctgccgttga ggggaaaacc gccgccgaat tgcaggcaca gtcgccgctg
    37861 gcattgtttg atgagctggg attgcgtgcg cagcttagcg cctcacgtag ccaggggtta
    37921 aatgcgttaa gcgaggcgat tatcgctgcg acgaagcagg tttaagcaca gaaccgggtc
    37981 ggataagacg ttcgcgtctc atccgacctg attgtttaac cctgacgcgc cgctttcgcc
    38041 atcatcttct tcagcgcatg agaaaccgca acaaacccaa aggtggcggt caccatcgtt
    38101 gccgcgccaa atccagatgc acaatccatc cgctttggcc cttctgccgt ggctttcatc
    38161 gcacacaccg taccgtctga ctgcgggtac accagcgctt cagtagaaaa cacgcaatcc
    38221 acgccgagct tacctttact gtttttcact acgccaaaat cgcttttcag gcgctcgcgc
    38281 aacttcgccg ccagcggatc ctgaatcgtt ttcgccagat cggtaacctg aatctgcgtc
    38341 gggtcaatct gcccacccgc gccaccggtc gtgaccagcg ggattttatt gcgccgacaa
    38401 taagcaatca gcgccgcttt gggccgtaca ctatcaatgg catcaatcac gtacgaataa
    38461 cccacgctca tatactgcgc tacgttatcc ggcgtcacga aatcatccac caccgttaca
    38521 cggcactccg ggttaatctg gcgaatacgt tccgccatga cttccgcttt tgccagccca
    38581 acgttatcgc gcagggcatg aatttgccga ttggtattgg tgacgcacac atcatccata
    38641 tcgataagcg tgattgcgcc aatccccgtg cgcgccagcg cttccgccgc ccaggaaccg
    38701 acaccgccaa taccgaccac acaaatatgc gcgtcagcaa acagttgcaa cgctttttca
    38761 ccatacagac gcgctgtgcc accaaaacgc tgacgccatg catcactaat taccacagac
    38821 atagcacctc agaattaaaa agggcgaggt tatccctcac cctaaacgca taaatgcctg
    38881 atgcgctacg cttatcaggc ctacataact cctgcaatat tttggatttg catgatttgt
    38941 aggccggata aggcgttcac gccgcatccg gcatgaacaa cgcgcactta gtcaataatc
    39001 aggatgtgag gttacccctc accctgtcat atccgtaaaa acggcataca gaatatcaca
    39061 tcagccgcta aagacgttac ctgcgcccgg ggcggttttc agcacccaga cacgtccata
    39121 gtggttgtac caacctgcgc ggtgcccggc ttccggcccg atcccttgat agatatcgaa
    39181 gtgttggcct ttgattgcgc caccgacatc cagcgccacc atcagacgca attcgtactg
    39241 accgttaaat ttgccgttat tatccagcag cggcacttct gccagcaagg tagtacctgg
    39301 cggaataatt gaacgatcgg aggcaacaga agcacggcca accagcggca ccgcacttgc
    39361 ccctttcacc ggagcaaaag attgcggttt aaagaagacg aaagacgggt tctgttccag
    39421 cagctcgcga acctcggctt cactgtgtgt ttcgccccag tgacgaatcg cctgcatcga
    39481 catatcttct tttttcactt cgccacggtc gatcagcacc ttaccaatgc tgcgataggc
    39541 atgaccgttt ttccctgcat agctgaaaaa gttaagcgga ctgccatcac caaagtcgat
    39601 atacccacta ccctgcacat ccataatgaa gttatccatc agggagttac tgtaagcgag
    39661 aatatattta tcactcaatg cccccgcgta gatctccgca cgagacggca gacgaccacg
    39721 ttttggcggc atacggtaaa taggatactg gaactcgccc tggcgggtat ggcgcgcctg
    39781 aattaccggc gtgtaataac cggtaaactg cacgttacca tagttgtcgg caccttccat
    39841 ctgccaggca tcaatgccga actggcgcat attgcgggta tcaccgcctg cgcgcagcca
    39901 ctcttgcacc gcgttataaa cattactctg gttgccatac agacgcggtg acgaattacg
    39961 gatatggtta atttgctcgg caaaatcacc ggcgttaatc ggcgcgccaa cggcatctgg
    40021 ctggttcacc agagagaaag gctgggtaaa tttcccgtct ttatattgct gtccgcgatc
    40081 ggttggtttg gaagagcagg cggcaagcat tgccacaacc gtgcccataa gaaggtactt
    40141 tacccaacgt cctttcattg ttctcttctt caggttaaaa aataaggcgc aacgaagata
    40201 acaaaccgcc gcgtttaatg aaatgagcag cctctccctg acgcaaattt tgcacaaaaa
    40261 ataggcttta gtgatttgtt tttgttcaaa atcatgccag atccgtgatc ggggtaaaaa
    40321 aaaggttgca tgaaaacgcg agcggagtat agtgcgcatc cacggacgcg gggtggagca
    40381 gcctggtagc tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa
    40441 ccaattaaaa tttgatgaag taaagcagta cggtgacgcg gggtggagca gcctggtagc
    40501 tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa ccaatcaaat
    40561 ttgatgaagt aaaagcaata cggtgacgcg gggtggagca gcctggtagc tcgtcgggct
    40621 cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa ccaattattg aacaccctaa
    40681 cgggtgtttt tttgtttctg gtctcccata aaaaaagcgc cattcagcgc ctttttatca
    40741 tccccttctc gccagcgttg ccccatcggc aaaatacgct ttaatccccg caagaataga
    40801 ctccgcaact tcctgctgga aagtcgccgt tttcaatttg cgctcttcct caacgttact
    40861 gataaacgcc gtttcgacca gaatggaggg aatatcgggc gctttcagca cagcaaaccc
    40921 ggcctgctca acctgattct tatgcagttt gttgattttc cccagcttat ttagcacggc
    40981 cttaccaaac ttcaggctgt cggcaatggt caacgactgc accatatcga acatggtgtg
    41041 gtcgacatag cggtcgccgc ttttgctcac gccaccaatc aagtccgagg cgttctgggt
    41101 ttgtgccaga tattttgccg cagtactggt tgcgcctttg gttgagagcg caaacaccga
    41161 ggaaccgctc ggctggcgac tggtaaaggc gtcggcatgg atagagacaa acaggtcagc
    41221 acgctgtttc tgggcttttg ctacgcgcac ttgcaacgga atgaagatgt cttcattgcg
    41281 cgtcatgtac accttcatat tgccctcttt ctcgatcaga gagcgcaaac ggcgagctat
    41341 ttgcaatacc acgtcttttt cgcgcgtttt gtatttcccc accgcaccgg agtcttcgcc
    41401 accgtggcca gggtcaagca taatgacaat cggacgatcg cgccctgctt tacccggttg
    41461 tggaccactt tgcgctggcg gcacctgctt ttcgaggtcg cctttgttgt aatcctccag
    41521 cagcgccagc agcgggtcct gcatatcctg tgcattggcc ggatagaggt ccatcaccag
    41581 acgctcttta aacccggcga ccggcgcaag ggcaaacagc tgcggtttta cgttttgctt
    41641 taattcaaaa accatgcgta cggtttgcgg gtcaaattgc ccgacgcgcg ccgacttgat
    41701 gaacgggtcg tctgcacgaa tttgtgccgc catccccttg agtaccgagt tcaggtttac
    41761 atcttcgata tccaccacca cgcgttccgg gttactcaac gcgaactgct tatatttcag
    41821 ctgacgattt gattctaccg tcacacgggt gtagctggac gcaggccaga cgcgcaccgc
    41881 cacgacctgg ctaaccgcag ccaggctgac ctgacttacg ctcaatagcc acatggcgcc
    41941 cgcgccttgc agtaaacgac gacggctgat tgcagtgttg gatcctgaca tgcctctccc
    42001 gagcaaaaga aatctaagct gtgtaacaag taaacgacta atttgaccgg aaactttagc
    42061 gaaagacgca taatctgtca tctaataaac ggtaaacatt ctttttatat tcacggcatt
    42121 actgataaaa aagtcgctct cgcataaaat ttacacttgc accctgcgaa aaaacagaat
    42181 aaaaatacac taatttcgaa taataatgca aagaggtgtg ccgtggtaaa ggaacgtaaa
    42241 accgagttgg tcgagggatt ccgccattcg gttccctata tcaataccca ccggggaaaa
    42301 acgtttgtca tcatgctcgg cggtgaagcc attgagcatg agaatttctc cagtatcgtt
    42361 aatgatatcg ggttgttgca cagcctcggc atccgtctgg tggtagtcta tggcgcacgt
    42421 ccgcagatcg acgcaaatct ggctgcgcat caccacgaac cgctatatca caagaacata
    42481 cgtgtgaccg acgccaaaac actggaactg gtgaagcagg ctgcaggaac attgcaactg
    42541 gatattactg ctcgcctgtc gatgagtctc aataacacgc cgctgcaggg cgcgcatatc
    42601 aacgtcgtta gtggcaattt tattattgcc cagccgctgg gcgtcgatga cggcgtggat
    42661 tactgccata gcgggcgtat ccggcggatt gatgaagacg cgatccatcg tcaactggac
    42721 agcggtgcaa tagtgctaat ggggccggtc gctgtttcag tcactggcga gagctttaac
    42781 ctgacctcgg aagagattgc cactcaactg gccatcaaac tgaaagctga gaagatgatt
    42841 ggtttttgct cttcccaggg cgtcactaat gacgacggtg atattgtctc agaacttttc
    42901 cctaacgaag cgcaagcgcg ggtagaagcc caggaagaga aaggcgatta caactccggt
    42961 acggtgcgct ttttgcgtgg cgcagtgaaa gcctgccgca gcggcgtgcg tcgctgtcat
    43021 ttaatcagtt atcaggaaga tggcgcgctg ttgcaagagt tgttctcacg cgacggtatc
    43081 ggtacgcaga ttgtgatgga aagcgccgag cagattcgtc gcgcaacaat caacgatatc
    43141 ggcggtattc tggagttgat tcgcccgctg gagcagcaag gtattctggt gcgccgttct
    43201 cgcgagcagc tggagatgga aatcgacaaa ttcaccatta ttcagcgcga taacacgact
    43261 attgcctgcg ccgcgctcta tccgttcccg gaagagaaga ttggggaaat ggcctgtgtg
    43321 gcagttcacc cggattaccg cagttcatca cggggcgagg ttctgcttga acgcattgcc
    43381 gctcaggcga agcagagcgg cttaagcaaa ttgtttgtgc tgaccacgcg cagtattcac
    43441 tggttccagg aacgtggatt taccccggtg gatattgatt tactgcccga gagcaaaaag
    43501 cagttgtaca actaccagcg taaatccaaa gtgttgatgg cggatttagg gtaagcgacg
    43561 aaaatcgccg gatgcgacat gcgtaacact cgtgcgtcgc atccggcaat tacgtttatt
    43621 cccgcgaact aaacaacgcc gccagaccac tgcgccgctc agtacgagtg gcgattgccg
    43681 cacttaatat acgctcatcg gcatacagcg acagacggcg acgcgcgcgg gtcaccgcgg
    43741 tgtataccag ctctcgcgtt actaccggcg tgcgttggct cggtaaaatc aacgccgcat
    43801 ggtcgaactc cgatccctgc gatttatgta ccgtcatcgc ccacgtagtt tcgtgctctg
    43861 gcaagcgact cggttgcaca gacttaatat tgccgtccgg catcgcaaac cagacgcgcg
    43921 tcccctgccc gcgatccagc gcaataccga tatcgccatt aaacaaccca agcgcgctgt
    43981 cattacgggc aatcatcacc ggtcggcctt cgtaccaacg agagtgcgga tgacgatgaa
    44041 ttttgcgctt ctgttgcata aactgctcaa ttcgctcatt cagtccagcc acgccaaacg
    44101 gcccttcccg cagggcgcac aaaagctggt actcattgaa cgcctgaatg attaaatccg
    44161 gctcggcacg cgcttgcagc agatccagat aacgtccgta acccgcaaga gcttcctcaa
    44221 gcatcgcaat ataatcttcg ccgctctgta aaagtcgttt ttcgatatca gtaaaatcct
    44281 gctgaaaaac ggttttcact gccgttttat caccacgatt gatcgccgca gctaactgac
    44341 caatgccaga atcgctgccg aaacgatagc ttttttgcag caggcagaga ctgtcgcgca
    44401 aagatgccgc ttctgtgccg gttcctgccg gaacgtgagt ccccgtcagg cggcttagct
    44461 gcctggcacg ctcggcggta aagcccgcgt tggcataagc gcagatatcg cccagcacag
    44521 ccccagcctc aaccgaggcc agttgatcac gatcgccgag aaagatcact cgcgcatgat
    44581 ctggcaaggc gtcgatcagt ctcgacatca taggcagatc gatcattgac gcttcatcta
    44641 ccaccagcac atcaagatgc agcgggttac cggcatgatg acgtaaacgc tggctacccg
    44701 gctgcgcacc cagcaatcgg tgcaaagtgc tggcatcttc cggaatgcgt ttcttttgtt
    44761 catcgctcag cggtaactgt cgcaaagcct tgccgagaga ttcggttaag cgcgcggcag
    44821 ctttacccgt tggtgcagcc agacggatac ggcagcgttc gccgtcggcc atttgaatta
    44881 acgctgccag caacttcgcc acggtggtcg ttttaccggt gcctgggccg ccggaaatca
    44941 ccgagatccg ccgcgtcagc gccactgccg ccgcaacttt ttgccagtta atttcatcgc
    45001 ttactggaaa aagtttgtcc agggtttgcg ccagtagagc ttcatcaacc tcgatggcat
    45061 gattcacctc attgaagaag cgcgctaccg tccgctcgtt acaccacatg cgattcaaat
    45121 aaagacgatc gccacagagg atcatcggcg tgggttcatc tccccggctg accgcttgtg
    45181 aagccagcaa gcattcttcc caattttgta gctcaccgat ttcactgaca caggtcgcca
    45241 acagcggatg cgacgcctcg ttattttcca gtcgtgaaag cggtaaacag acatgccctt
    45301 ccccggcgtc gtggcttaac agggctgccg cgagggttac ggcaggatgt tcatctcccg
    45361 ccacggtcag ggcaaattgc acatccagcg ggcgtagctg tttgtgctcc acagcttcca
    45421 gtaattgctt ttgcaatttc attacgcctc ctccagggtc ataccggcaa acatctcatc
    45481 catcaggtca atcaaccctg cattgggtcg ggttgtgtaa atcccctgtt gcggatgttc
    45541 tttatcaacg ccacgcagga acagatagat aacgccgcca aagtgacgat catagtcgta
    45601 atcagcaatg cgatggcgca gataacgatg cagcgccagg gtataaagct gatattgcag
    45661 atcatagcgg tgtgcctgca tcgctgctgc catagcctgt tgggtgtaag ccgaactgtc
    45721 ttcacccaac cagttggatt tatagtcgag caggtaataa cgcccttcgt ggcggaacac
    45781 caggtcgata aagcctttta acatgccacg tacctgcatg aactccagcg gcgggcagcc
    45841 tgcggatagc gggtcaaact ggcggattaa cgtatcaagc tgactggcga taagcggttc
    45901 actaatcggc agataaaact ccatctccac ctgtttattg cgggcggaaa gttgactcag
    45961 gcttacgccg gtttcattga gaggtgcctg gaggacagcc gtgatccact cggtcaatac
    46021 cggttcccac tgcgattcaa agccgccgag ttccagtttt tcccgcaccc agttcgggtc
    46081 aaccggctgg gtaaaatcca ggtcttcaaa caaactgtgc aagaacgtcc ccggtgacgc
    46141 accgcgcgga aactgatgtg gtgttaacgt cggttcttca acgacgctgg caacgcctgc
    46201 agcatcgaca tccagccgag gcatcaaatc ctgggcgata ccgtgaccac gctgttgcaa
    46261 accagagtag ctggtgacgc gccagttatc gccgggcaat cgttgtaacg tcttcgcatt
    46321 cagctctgct gtagaaacat cattaacctg ccagggttgg ttatcaccaa tttgtgccgt
    46381 ttgccaggca atatcatcat cgcacaatgc ttcaatacag gtgcgaagcc ctgccgcatc
    46441 ttgcggttcc cctttttgca gcaaacgccc gagcgcactt tggtggacgt cggtgtcacc
    46501 ttttttatcg ccacgacggc gcaccagcgg tgcaacgccg agactacaat gccaaaccga
    46561 acgcgtcagc gccacgtaaa gcaaacgcag atcttccgcc agacgttcgg cctccgcgag
    46621 gtcgacgctt tctggcgcag cattaagatc cagaactgcc tcaaacgagt ggcgatcgtg
    46681 ataaaacgcc tgctcctgga cgcggaaatt ggtgataaac ggcagccaga ccaatggata
    46741 ttccagccct ttcgatttgt ggatcgtgac aatctgcacc agatgtttat cactttcgag
    46801 acgcatttgt tggctggagg cattactgtc tggctcgagg atatgttgcg ataaccagcg
    46861 taccagcgca tgttcacttt ccagctgcgt tccggcttct tgtagcagtt cgctgatatg
    46921 caagatatcg gtaagacgcc gctcaccgcc tgccgttgct agcaagtttt ctgcaatgtt
    46981 acgcgccgac atcagcgccc gcagcatcgg cataacgcca cgtttgcgcc agatttgccg
    47041 ataaccatcg aactcttcaa ctaccgcatc ccacgcatgt tcgtcattat tcagcgtttc
    47101 gatatccagc gcgttcagcc ccatcattga cgttgccagc gcactacgca gggtgttctc
    47161 acgttcgggc gtcatcaccg cctgcaacaa ccaaagcatt tcctgcgctt ccagagtttc
    47221 aaaaacactg tcgcggttcg aaaggtaaac ggaagggatt tccagcaacg ttaaggcatc
    47281 gcgcacctgg gcggcctcct ggcggctgcg caccagcaca ctgatgtccg aagcacgcac
    47341 cggacgcgcg tcgtcgccgt tcatcagcaa cgcttcgccc ctctgtccgg cttgtagcca
    47401 gtcgcggatt tgcgcagcac atacctgcgc catggtactt tgataatcgc caacgccgca
    47461 gctttcgcct tccatcagcc acattttcat cgcaggctgc gtttcacctt taaatacaaa
    47521 acgtaacgcc tgatttttcc cggctgattt cactggaata aacggtattt cgcgaaacat
    47581 gaacgcgtca tcagtctggc tgaaaagctt attcacgctg ttcaccattc ctggtgcgga
    47641 acgccagttg gtgtctaaag tgtagtgggc gtgaacttcg ctacgcgcct tcatataagt
    47701 gaagatatcc gcaccccgga atgcatatat ggcctgcttc gggtcgccaa ttagcaacaa
    47761 tgcggtttcc ggctgatggt gccagatacg gcgaaaaatt cggtactgct gggggtcggt
    47821 atcctgaaat tcatcgatca ttgccaccgg gaatcgcgta cggatcgccg ctgccaacac
    47881 ctcaccgctt tcgctacgca gcgcggaatc gagccgactt aacatgtcat caaaacccaa
    47941 ttcgccacgg cggcgttttt cacgcgctac tgtttcgcgg atctcagcca atgcgcgggt
    48001 gatcaccaga tcgcggatcg acaatggttc tgcaagcagt tgatcgatcg cctcaaacag
    48061 tggatgtcgc ggggtttccc ccccggcctt cgtgcgatct tctaagaaac gctgggagaa
    48121 tttttccagc gactccggca actgataact gtttgtctct tcttctgccc aggcgctgat
    48181 cttgtcgatc catttagcct gattgctacg gttaaacttg cgtcgatcaa taccagaaga
    48241 ttcgatcagc gcatccagtt cacccactgc gtcgcgccac tgctgtttta ccgtatcaat
    48301 acgcgccaca atttgcgcgt gacgggaagc cagcgtttca tcatcgggcg gcggtgcttt
    48361 gataaccggc gcttcgcctt gcagataacg attaatatcg cgcagcaacg cctgcggccc
    48421 tttccaggtt tcaaagacga cctgggctat ttcacgcggc agcgggtagc agtggcgacg
    48481 ccagaaatcg gcgcaggcct ggtagcgtag cagagactca tcttcaatca gctgctgctc
    48541 aaacagcatg ccggattcaa aggcattcag gttgagcatg cgctggcaaa agccgtgaat
    48601 agtaaagact gccgcttcat ccatctgccg ttcggctaac aacaaccact gcgcggcttg
    48661 cgctttatcg tcgatctctt ccagcaggcg ttcgtacagt ggattgtcgg tggtttcacg
    48721 cagacaggcg atgcgcaact cgtggatatt gctacggata cgaccgcgca attctgccgt
    48781 ggcagcctcg gtaaaggtga ccaccagcag ttcttcaacg gtcagcgggc ggggaaaggc
    48841 ggcggaaccg cctagtccaa gtaacaggcg caaatagagc gccgcaatcg taaaggtttt
    48901 gcctgtgccg gcagaggctt caatcaggcg ctcaccctgt aagggcaagc gcaaaggatc
    48961 tagtgtctcg gcgacatcac tcattctttt cactcatcag gggcattgtt tgctgcaacg
    49021 cgctgacgtt ctcccacact ttccagcctt cagggtgtac atattcggct ttcccgttct
    49081 ggctgccgga aatctgcgac agaatagcca tgccttgcgg ctcgaccacc gcctgatgga
    49141 agaaatcagc aagtttttgc ggcgtcagca gttttatctg ggccacgatt ttatcacgcg
    49201 aatcgaagcg catattgccg cgatcgaaat ctttacttaa cttcgatgct tcttcgccga
    49261 gcgtttgcgg tgcctgcagc atctgggtaa ttaccgcctg ctggatttgc gcaaactcat
    49321 ctggcttcat cgctcgcaat tttgcctctg cggttgggaa aaacgccttg taacgctccc
    49381 acaagaatga aggctgttta tcattgcttt gcaaaaggaa gcccatgccc cactgacgcc
    49441 ccacgctcat tggaaacgca aacacggcat agcccaattg ttcttcggta cgcaactgat
    49501 tgtagaacca cggctgtacg atctgcccca acagagagct ataggctgag ctggtgtatt
    49561 catcgtagcc agtcggtaca aataccgctg ccagtgcgga gtcggtgctg ttaccggctt
    49621 tttcaaagat gacggattgt tttttatcga ccactacatc tttgtttcga caccactctg
    49681 aaccatcagc gcccaactgt ttttgcacat cgcgtgccag cgttgttgcc tgggcctcgg
    49741 tcatgttgcc gataaccata aactctggtc gagcccctga ttttaaggcg tcgcgatagg
    49801 ccagcacctc tttcaacgta atggagggca aaattttacg ccgttcatct cgcgagaagt
    49861 acggcacttg cgagagcatc tgcgcgggca taatcgcctg ctcaaacgct ttaccctttt
    49921 ctgcggaatc catcatctgg ttataccagg acttcgcctg ctcaagctga tcttccgtgg
    49981 cggtatagct aaagtagcct tccagcaatg cctggaacag ctgcggcaga cgctgggtgt
    50041 aaccattagc attaaccata aggccgttgt tagcgttggt ggaaaaactt atgccaccaa
    50101 ccgacgcctg gttgcttaac tgatcaagcg ccagccctgc gagataatca ttgagcgcaa
    50161 acatcacctg attgcgggcg ctgtccatgg ctttcggatt acgcaaaatc aggctgacat
    50221 cggcttttgg ctcgctggca aaataacggc ttggcgcata cactacgcgc agattcgact
    50281 catcaacaat cagctctgga tggtcatatt tcttgtctga cttaatcagc gagaaatcgt
    50341 caggaatata agggttaagc tctggcaaag agagcgcaat gtcggcggct tttttctgcc
    50401 agtcggcgaa agtttgtgcg ctgattttat cgacctgata cggcgcatcg acaaagtaag
    50461 ccgttttgtt gtgcggctct ttcgggctga tataccagat acgcgcattc tgcggcgtca
    50521 tcatcgccag acgttccttt actgctttag catcgtaccg atcggcaata ttgactgcat
    50581 ccagcgtatg ctcaacagga acgcgaatca tggtatctgc cagccattcg acgtaatcca
    50641 tatcacgggt gattgacgga taacggaagt cgatatccag cacattcgcc agttcatcga
    50701 agtattgttt atcgatccct ttttcacgta acaaattgag gtagctaaaa atagccgcca
    50761 caacctgatc gcgattcgcc agacctttat cggttaaaga cgcagagatc gctaatacgc
    50821 cgctgttgcc gttgacgata ggatcggagt tggcgctaat gccctcaact aatccctgct
    50881 tttgcagcca gtcggaaagt gtacctgggc tgcgattacc aatcagatag gtaatcaatt
    50941 catcggtttt gctacggaat ttcgccgagt tgttatcaat acgaaactct acgcgcaaca
    51001 ctttacgcgg cagcgccggg acgtaatgaa tgataatgcc cttttgcgcg tcggtgacta
    51061 ccggcacggt gatttccggt tttttgctct ctttgttcgg cacgcgacca aaggtgtccg
    51121 ccgccatttt tgccaactcc ggcagcggtt tattgctgta aataaccgcc ttcatcagat
    51181 tggcggaata gtacttctcg tggaaatctt tcagcgcctg ctgcaccgga ttaccaggtt
    51241 tgtcgcttaa agtttcgagg ttaccaccag aaaactttga accggggtgt gccgggttaa
    51301 tggtttctgc gctgacctgc gccatgcgca tcccgtcacg cgtacgcgcc atggttaatt
    51361 cggcattcac tgcattacgt tcacgttcgg cgtatttctt gtcgagcaag ggttctgcaa
    51421 tagcgtccgc caggcggtct accgcaccgg gcaaggcgtc gttctcaact tccagataga
    51481 aagccgtgcg atacggtgcc gtgctggcat tgtgactgcc gccgtgcatt ttgagatatt
    51541 cggccagact gtcagcctgc gggtactttt tcgaccccat cagactcata tgttcaaggt
    51601 aatgtgccag cccctggtac gcctcgggat cttccagcga cccaacgggc accaccagcg
    51661 ccgagagcga tttaaccgcc tgcggatcag aaaccagcaa gaccaccata ccgttatcca
    51721 gacgtatagc ctgatactgg cggttatctt tatcactttt acggatggtt tcctgaatcg
    51781 gctgccatcc cgtttctgcc tgacttaagg gtgcccaaag ggcaacaaac aacaataatg
    51841 ctttgaacca ggtgctgcgg ggcattcacg gacctcataa tcaactttat tttctggcca
    51901 gattcaacag gttaacgcaa atcatctgcc agaatttaat cttgtgctgc acgagtcagc
    51961 ctatgtctat atatccatca gtccgtgact ggtgcgcatc ataatgtaag cgaatgaatt
    52021 gcgcaatttt tatacagcac tcatgactga ttaaagcgaa acagcggtaa caggaaacgt
    52081 tgcgactgtt caacgatggc ctccattgtc tctggtgtta attgccgcca gagccgttga
    52141 taccagatat catcaccttc gccacgcacc atcatgttgc cttcgtaagc ctgaaggaat
    52201 ttcgtacggg ctttttgcaa cgtggaatcg tcatccagca tggcatcgtt ttgcgcgtca
    52261 taacaggttt ttagccacgc gccgccactt tcaggtaaca ccagcaatgg cgcggacatt
    52321 ccttcacgat acccctcaat cagttgtgag aggtaatgca aagcctgttc ggctgcaagc
    52381 ggcggaaaac gccactcacc gtctttgcgt agaaaaaggc gactttcgcc attaccaccg
    52441 ctggcacagt agacaaggtg ttccagccaa agttgcattc cctgcgccac acttaataaa
    52501 gagggacgcc agcgcaacag gccatccggc tgcacctgcg gcaaccagcc agttatctgc
    52561 acaccgttgc aggcgagatc aatttccata ctctgccccg gctggcgaca ggcaatgact
    52621 ctgtcggcaa gctgctgcat ctcctggcac tgtgtttccc agaaaatttc accaaaagcg
    52681 ccatacggta aatcccctgc cgcccggaag cggcggaaca agcgttcggc atcatcctgc
    52741 tcaaccagtg cattcaataa ctgctgattg atttgataac ggctaagtcc ttccagaata
    52801 aatggctcgg tgtctgggat ttcgctgtct tcagtacgga agttcacctg caaacgcatc
    52861 tggaagaacg cccgcaccgg atgtgcccag aatcgttgta gcgtttccag cggcacggtt
    52921 tccggtaagg taaacggcag cggctgaaca aattcagaat gtgctttacc agcctggctg
    52981 gccgcaggta gccattcacg agcatagctt tgtcgttcgc ctggctggta gttttgtgga
    53041 tcaaacggca tccgggtatg gaggcaagta agatgcgctt ttacccttgc ctcgctttca
    53101 tcacagttga gcgcttcatc gcccggtaga taatgacttt gcccgatgta gtcgatcagt
    53161 tcctgcacca gtaccgacgg gaaacgttca ctgttatcct gaatggaacg cccgatatag
    53221 ctgatataga gtttttgctg cgcggaaatt aacgcttcca ggaacaggta gcggtcgtca
    53281 tcgcgacggc tacggtcgcc acgcttcggt ttctggctca tcaggtcaaa gcccaatggc
    53341 gcaagctgac gtggataaac gccgtcgttc attcccagca ggcaaaccac tttgaacgga
    53401 attgaacgca ttggcatcag agtacaaatg ttaaccggtc cggcgagaaa acgctggctg
    53461 atacgttctt gatccagacg ctgcgccagt tcatcacgca atagtgacag cggcaccgcg
    53521 tcgccatact gcgcacctaa accttcggcg ataatcgcct gccattgttg ttcgatcagc
    53581 gtcatcgccg cttcggtttc cgcatccggc aggaagaagg cgttgagcat atcgcgacaa
    53641 accggcaacc actcttctag cggacgctcc tgtgccagcc cgcgacgcca gatgtttagc
    53701 tgcattagca gtgaagccag atgccccacc agttctgcaa ttaagccgct cgattcatca
    53761 taaggtagaa ccgattgcca ctcgccctgc gcgctctcca tcgcgtagcc cagcaacata
    53821 cgcgtcaggc caaatcgcca ggtgtgttgt ccggtggcgg ggagttccag ctcgcgaacg
    53881 ttgtcgtcat ctatccccca acgaatgccg gattcgttga cccactggcg taaataacgc
    53941 agcccttctt cggtgatgtc aaaccgcgcc gccagcaccg gcacatccag cagcgccagc
    54001 acatcctctg acacaaagcg actgtcaggc agtgataaca ggctgataaa cgcttccagt
    54061 accggatgtg actgccgcgc acgacggtcg gaaatggcgt aaggcaggta acgatccgca
    54121 ggtgcgctac caaacactgc ctgaataaac ggactgtagc tgtcgatatc agccaccatc
    54181 acgatgatgt cgcgcggagt aagtgtcggg tcttcctcca gcatcgccag caagcgatcg
    54241 tgtaaaactt caacttcacg ctgcgggcta tggcaaacgt ggaatgtgat actgctatcc
    54301 agtggatcaa gcgggcgttt gttatcgcta cgggaaaact cttcgatgtt cacacccgca
    54361 acggcgcggt tttccagttc cagaatgtca gactgaatgt tatgcagcag gttatctggc
    54421 gtgacatcga caaaggcgtc cagctcctgg ctgctctcca ggtcagaaag gagataaatg
    54481 tagtcgcgcc caagcttacc ccatgaagcc agcagcgggt tgccgacatc ctgttcacca
    54541 tcgctgttaa agagctgccc ggcattttcg ctatcacgaa atagcggcaa ttcgcgatct
    54601 tcaaaactgt gtcgacgctg acgggtcagc agtttcgcca gataagcagg atctttaata
    54661 tcgccccagt aataacggca ggggttggta aacaggagat ggatttcaat atgtttaccc
    54721 agcgcctgga gcgcctggag ataaacaggc ggtaacgcgg aaataccgca tataaagacg
    54781 cgcgaaggta accccggcgg gcaggtcgtc gcggactcca gcgtttcgat aaagcgctga
    54841 tagagattgg cgcggtgcca gcgcggttgt ccgagttcat gggtatattc caccagcgcc
    54901 ttccacaacg gcgcttgcca ggcctgtgct tctcccagcc cttcaaccaa atgtcctgtt
    54961 tcccattgtg ccagccactc cgggcgatag accagatact ggtcaaacag gtccgccgct
    55021 tttgaggaaa gctggaacag ttttcgcttg tcgctatcgt cagtcagata atgccgcaac
    55081 agggtaaagt cttcgcgctc cagcaattgc ggcagcagag tcatcagttt ccagctcatg
    55141 ctctgtttgt taaaggcgct ctctttgggg atttccggta acacccggac gaacatatcc
    55201 cagataaagc tcgctggcag cggaaaatca atgtttgccg caataccaaa cttttgcgac
    55261 agggtcattt gcagccactg cgccataccg gtactttgca ccagaatcat ctctggttcg
    55321 aaaggatcgt ccagccgttc gcgttcgaca ataaactcca tcaacgcttc cagcacgtcc
    55381 agacgattgg aatggtagac ccttaacata gcggctcctg actactgacg attcgggcaa
    55441 tgcaggcggg tcatctcgcc ttctctgccc ccgggtgaaa ctagcgtaac gctgatgctg
    55501 acacatcccg cctgcgatgt ctgcattcgg ttgacctgcc agttggcagg tggcgaaatc
    55561 gcgcgcagtt gcgtttgctg ccaaccatgc cgccagagct gttggtactg gtttctgctg
    55621 gcgagactgt tcattaatgt tcgctggata cccgataacg cagtgacaat catcaccatc
    55681 agcaccatcg caaccattac ctccggcagg ctaaagcctt gttgattctt cagggaagct
    55741 gacataatgc cccctctttc agcggacaaa aatcgctcca gccgcgtggc gaaaaaacaa
    55801 tttttccatc aatgacttcg cctgttcgcc acaacgaaac gccttcataa ccggcaatca
    55861 ataaggcttc attatctgcc agtaaacgca aacaaacctg ggcatcggtt ccagcgtact
    55921 gcgagcactg aactgctggc tgcgtctgcc aggagtgcat ttttccccac gccagcgccg
    55981 actgaacgat ggcctggcgg cgcaatgact gactttccat gctcacgcga gaggcaaaac
    56041 tgcgatcctg ctgactcatt ccttgcaata gcaagctacc caaaaccagc agcatcagga
    56101 ccagagccag tgacgaaaca cctttttcgc ggttcacagg ttgaatcctg tcacgctata
    56161 gctggcatcc accacggttt gcggttcaga cttactggca gcacgcatat taaccgtcaa
    56221 caccggcgag aagccgctga catcctgacg tacgacctga aaagtgtcga taatgatggc
    56281 atccggatta gtgactttat cccagccctt accttcacag gatgtcgcac cgcgtagcgt
    56341 ttccagcaca tgctccttca gacgaaatcc aatctggtcg gactctttta ccggttcgcg
    56401 atcccagata ccgttactgt tcgcatccca ctgcacaatg atacagtcac cctgtccgac
    56461 aatttccagc ccttcgccgg tacagctgcc atgacaataa cccgccctct ggagatgctt
    56521 cgcgacggta aatacccgca gccagatttc atcttccagc gccagcttac gggtgcccgt
    56581 taaactttca cgctgtaacg caggcagaaa gcgtgccgct cccagcaaca atacgctact
    56641 gatcgccata gcaatcaaca cttccagcag agaaaaacct tgctctttta caggcatcct
    56701 tctgtttctc cttgctgaca aagccggagt cttccccacg gcgaaaccac cagccaccac
    56761 tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg cggtattgcg caggccaaag
    56821 aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg gccagcgtgg cacaaagacc
    56881 aatggtgaac tgccatgaca ggtattcgcc ccagcaacgg aactcacaag gcaccataac
    56941 gtcccctctc tgataacact gatactgtgg tcgcggttat gccagttggc atcttcacgt
    57001 aaatagagca aatagtcccg cgcctggctg gcggtttgcc aaagccgttg cgactgctgc
    57061 cagtattgcc agccatagag tccacttgcg cttagcatga ccagaatcag catcgcgacc
    57121 agcgtttcaa tcagcgtata accacgttgt gttttcatgc cggcagtatg gagcgaggag
    57181 aaaaaaagac gagggccagt ttctatttct tcggcgcatc ttccggacta tttacgccgt
    57241 tgcaggacgt tgcaaaattt cgggaaggcg tctcgaagaa tttaatggag ggtaaaaaaa
    57301 accgacgcac actagcgtcg gctctggcag gatgtttcgt aattagatag ccaccggcgc
    57361 tttaatgccc ggatgcggat cgtagccttc aatctcaaaa tcttcgaaac ggtagtcgaa
    57421 gatggattcg ggtttacgtt tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa
    57481 ttgcagatga gtttgatcca tatgattgct gtacaggtgc gtgtcgcccc ccgtccagac
    57541 aaaatcgccc acttccagat cacactgctg cgccatcata tgcaccagta acgcgtagct
    57601 ggcaatgtta aacggcaggc cgaggaaaac gtcacaggag cgctgataaa gctggcaaga
    57661 gagtttgccg tctgccacat agaactggaa gaacgcatgg cacggtgcca gcgccatttt
    57721 atccagttcg cctacgttcc acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt
    57781 cagctggttc agtaccgtag tgatctggtc aatatgacga ccatccggcg ttggccaggc
    57841 gcgccactgt ttaccataca ctggcccgag gtcgccgttt tcatcggccc attcgtccca
    57901 gatggtgaca ttgttttcgt gtagataagc aatgttagtg tcgccctgca gaaaccacag
    57961 cagttcatgg atgatggaac gcaggtggca acgtttagtt gtcaccagcg ggaatccatc
    58021 ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc gttccggttc cggtacggtc
    58081 gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt tctaaatact gtttcatggt
    58141 tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc cagaccatca tgatcacacc
    58201 cgcgacaatc atcgggatgg aaagaatttg tcccatgctg atgtactgca cccaggcacc
    58261 agtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtaacc
    58321 aatcaggaac aaacctgaga cagctcccat tgggcgcggt ttacgaatat acaggttgag
    58381 gataataaac agcactacac cttccagcaa cagctcgtaa agctgtgatg ggtggcgcgg
    58441 cagcacaccg taagtatcga aaatggattg ccactgcggg ttggtttgca gcagcaaaat
    58501 atcttctgta cgggagccag ggaacagcat ggcaaacggg aagttcgggt caacgcggcc
    58561 ccacaattca ccgttaataa agttgcccag acgcccggca ccaagaccaa acggaatgag
    58621 tggtgcgata aaatcagaga cctggaagaa ggaacgttta gtacggcggg cgaagataat
    58681 catcaccacg ataacgccaa tcaggccgcc gtggaaagac atgccgccgt cccagacacg
    58741 gaacagatac agcggatcgg ccataaactg cgggaaattg tagaacagaa cataaccaat
    58801 acgtcccccg aggaagacgc cgaggaagcc cgcatagagt aagttttcaa cttcattttt
    58861 ggtccagccg ctgcccggac gattcgcccg tcgtgttgcc agccacattg caaaaatgaa
    58921 acccaccaga tacatcaggc cgtaccagtg aagcgccacg ggtcctattg agaaaatgac
    58981 cggatcaaac tccggaaaat gcagatagct actggtcatc tgtcaccaca agttcttgtt
    59041 atttcgctga aagagaacag cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg
    59101 tgcgaataat agcacaaggg gacctggctg gttgccggat accgttaaaa gatatgtata
    59161 tgatccgcgc tataaccctc cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc
    59221 tgcaacctga tggcgaactt cggtcgccag ttgcgcttcc agactacgct gcgcaagatt
    59281 ttctgcttcg gcataatcaa tgcgccgcag caggtatttt acccgcgcta cagaacgtcc
    59341 gttcatagac agatggcgat acccaagccc aatgaggatt gccacgcaca tgggatcgcc
    59401 cgccatttca ccgcacaaac ggagatcgat tccatgtatt tccgcttccc gggcgatcat
    59461 cgccagagct cgtaacattg caggatgaag actgtcataa atgttcgcca cccgggtatt
    59521 gttgcgatca acggccagaa tgtattgagt cagatcgttg gtgccaacag agatgaaatc
    59581 gacccgcttt gccagatgcg gcagcataaa taccattgac ggcacttcca gcatgatgcc
    59641 gatacgtggt ttgggaattt cgtaaccgat catctcctcg acttcacgtc cggcacgttc
    59701 aatcaggcgg cgtgcttcgt caacttcatc gagactggtg accatcggca acagaatatt
    59761 caggttgccc gtagcggcat tagcacgcag catcgcccgc acctggatca agaagatctc
    59821 cggctgatcg agcgtaatgc gaatcccacg ccaacccagg catggattct cttcgctgat
    59881 cggcatgtaa ggcagctgct tatctgctcc gacatccagc gtacgcaagg tgacgggttt
    59941 atcattgaac atttgcagca tcccctgata ctgcgccacc tgttcttctt ccgacgggaa
    60001 accactttgc agcatgaatg ggatttcagt gcgataaaga ccgatgccat caatacggct
    60061 gcccagtttt tcttcatgtt ccgggcttaa accagcattg agcatgactt ttatacgctc
    60121 accgcttttt aactgcgcgg gtaaattgac gtcatcttcc gccagacggc taagctcaat
    60181 ctcttcacta attagccgct gatattcttg cagcagtacc ggctccggat cgaccagcaa
    60241 ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc agcaccgaag gctgaatatc
    60301 cgcgcccatc acggtaggga tccccagcgc acgtaccatg atcgcagcat gggagttggc
    60361 tgcgccatct cgcacgacaa caccgactaa gcgatcctgg ggcagctcag caagagttgt
    60421 cgctgacagt tcatctgcca ccagaatgaa acgttccggc caggcgttcg gcccttgatt
    60481 agcgtcatca agatgaaaca gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc
    60541 tttgagatag ttatcgctta gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt
    60601 taccgcccac tctgccaccg agcctttatc aacctcggca aacaattcgc gacgcagccg
    60661 ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc gccgtttctt tttgtgcacc
    60721 ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt
    60781 cagtcgttcg cgttccagag ccggatccag cgttgatgcc tgatacacct gttccattaa
    60841 aggtaacgtg gcatcctgcc agccttcggc aatcgccaca ccaggtgctg ccggtaatgc
    60901 gcggattcgc gtctggcgat attgcccaaa caaggcagtc aactgcgact gagaaagaat
    60961 agctgccatc tgggtggcaa gcgtcaccag gaaggattct tcactttcgt catactggcg
    61021 caactctcgt tgctgtacca ccagtacacc aagcaactgg cgacgttgaa taattggtac
    61081 gcctaaaaac gcgcggaaac gttcttcttt tacggagggg atgtatttga agctggggtg
    61141 cttttgcgca tctgcaaggt ttatcggttc cgccagcctg ccaaccaggc cgacgatccc
    61201 ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat
    61261 caggtagtaa caacgtcgat catgatcggc caggtagacc gaacagacct cggtatccat
    61321 cgcaagacag atgtcggtaa ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc
    61381 taccttttcg actatttcgc gcaggcgagt gagcataatt ggcgtgactt aacctctttt
    61441 acgtcgataa gcagatgcgt tttgtggttt tggcgtattt tcctgcagtg acatcaccac
    61501 actcgcgaac tctttcatta ccctacggta gacatcacgt ttaaatgaca ccacctgtct
    61561 gaccggatac cagtaactta cccatcgcca gccgtcaaac tctggtgtac tgctggtttg
    61621 catattgatt tctgcatcgc cgctcaccag ctgcaagaga aaccattttt gtttttggcc
    61681 gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc ggtaatttgt agcgcaacca
    61741 gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa
    61801 ttcacggtac atcgcctgct ctgcggattc tccggggttg attccgcctt gcggaaattg
    61861 ccaggagtgc tgaccaaatc gccgggccca cattacctgc ccctggcgat tacaaatcac
    61921 gatacctacg tttgggcggt agccatcgtc atcaatcacc ggactacctc aaaataaagc
    61981 tttatatacg aatgattgtt tcatactcca ggaagacggt aaaccactct ctgcagggca
    62041 ttacacacta ataacaattg aataactcac agttatgtgc agagttataa acagaggaag
    62101 gggtggatag ccgtttttat tcaccttttc tgtggataga gttgtgaaga actacggaat
    62161 tactacggga aaacccggag cattctgaat aagcccgata tagacatttt aaaatattca
    62221 ttattagtca atgcattaca tgtaatttga gtaatgaaaa agagtaattc gtgacccagg
    62281 tcacacctct catttacggg ttggcgaaag atcaaccaat gccgtattta tccacagaat
    62341 gtgccactaa gttaagcact gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg
    62401 taaatggaaa cagtagtgga ggtttttcac agttatccca gctttctgtg gataacatgg
    62461 tgtaagatcc tgtttatttt cagtgaccag atttggaaaa cccgttgcag tgttgcgcaa
    62521 ctcgattacc ggcaacctaa aaagcaatat aaatcagaga attgaacaac gcatgtggaa
    62581 aaagttacac tgcgaatatt cggcacataa ttgctgtttg ttttttaacc aaggtatcat
    62641 gacatgtccc aacctcgccc actgctctct cctcccgaaa ctgaagaaca gttgttagcg
    62701 caagcacagc aactttctgg ttatacattg ggagaactgg cggcacttgt cgggctggtt
    62761 acgccagaga atttaaaacg cgataaaggc tggattggcg tgttactgga gatctggcta
    62821 ggtgccagcg cagggagtaa acctgagcaa gattttgctg ctctgggcgt ggaacttaaa
    62881 actatccctg tggatagtct tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg
    62941 ttaacgggca atagcggggt gacctgggaa accagccacg tgcgccacaa actcaaacgc
    63001 gtactgtgga taccggttga aggcgagcgc agcatcccgc tggcgcagcg tcgcgttgga
    63061 tcaccgttgc tgtggagccc gaatgaagag gaagaccggc agctacgcga agactgggaa
    63121 gaattaatgg atatgattgt tctcggtcag gttgagcgga ttaccgctcg tcacggggag
    63181 tatttacaga tacgaccgaa agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc
    63241 cggggcgaac ggattctgac gctgccacgc ggcttttatt tgaagaagaa tttcaccagt
    63301 gcgctactgg cccgtcattt tctgatccag tagccatcgc tttgacctgc cgctttccgg
    63361 gcatataatt accgcttcat ttttttggca gggcttttta gatgttattt gcatggataa
    63421 ccgatcctaa cgcctggctt gcgctcggta cgctgacgct gctggagatc gttcttggga
    63481 tcgacaatat tattttcctt tctctggtgg tggcaaagct tcccacagca caacgtgctc
    63541 atgcgcgccg tctggggttg gcgggagcca tggttatgcg tctggcgctg ctggcatcaa
    63601 tcgcctgggt tacgcgcctg acaaatccgc tttttacaat attcagtcag gaaatttctg
    63661 cccgtgattt gattctgctt ctgggtggct tgttccttat ctggaaagcc agcaaggaaa
    63721 tccacgaatc cattgaaggt gaagaagaag ggctgaaaac acgcgtttca tcattcctcg
    63781 gcgctatcgt gcagattatg ctgctggata ttatctttag cctcgactcg gtgattacgg
    63841 ctgtgggtct gtcagatcac ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg
    63901 tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga acgccatcct tcggtaaaaa
    63961 tgctggcgct ctctttcctg attctggtgg gctttaccct gattctggaa agtttcgaca
    64021 tccacgtacc gaaaggttac atctacttcg cgatgttctt ctctattgcg gttgaaagcc
    64081 tcaacctgat tcgcaacaaa aagaatccgc tctgatactc cgtacgctct cctgcgggag
    64141 ggcgtactca ctccttttac cctcacagtt taagattttc ctgctttcaa gatatataac
    64201 gtcggtttat aaacagacta ttatcatagg taggccaaat gaggataacc ggatgaaaaa
    64261 atgggcagta ataatttctg cagtcggact ggcgtttgct gtttccgggt gttccagtga
    64321 ttacgtcatg gcgaccaaag atggccgtat gattttgacc gatggaaaac ctgaaattga
    64381 tgatgatacc gggctggtga gttatcacga tcagcaaggt aacgcgatgc aaattaaccg
    64441 tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg tcagcatccg gctggcctta
    64501 agatttttct ctttcccttt tcccttccct ctgccatttt tatattcctt atgtcgtgat
    64561 tataaaaagg aaacggctat gcaatatcac cgtatacccc acagttcgct ggaagtcagc
    64621 acgctggggc ttggcacgat gacgtttggt gaacagaaca gcgaagccga cgcccacgca
    64681 caactcgact atgccgtcgc tcagggcatt aaccttatcg acgttgccga aatgtaccca
    64741 gtacctccgc gccccgaaac gcaagggtta accgaaacct acgtcggcaa ctggctggcg
    64801 aaacatggca gccgcgaaaa gttaattatc gcctccaaag tgagcggacc gtcgcgcaat
    64861 aatgacaagg gcatccgccc ggatcaggcg ctggatcgga agaatatccg cgaagcgctg
    64921 catgacagcc tcaagcgcct acagactgat tacctcgatc tttatcaggt gcactggccg
    64981 cagcgcccga ccaactgctt cggcaaactc ggttatagct ggacggattc tgcgcctgcg
    65041 gtttcgctgc tggatacgct ggacgcactg gcagagtacc aacgagcggg aaaaattcgt
    65101 tatatcggcg tgtcgaacga aactgcattt ggcgtaatgc gctacctgca tctggcggac
    65161 aaacacgatc tgccgcgtat tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt
    65221 tttgaagtag gtctggcaga agtcagccag tatgaagggg tcgaactgct ggcctattcg
    65281 tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca
    65341 cgtaatacgc tctttagtcg gttcacccgc tatagcggtg agcaaacgca aaaagccgtc
    65401 gcggcgtatg ttgatatcgc cagacgtcat ggcctggacc ctgctcagat ggcgctcgcg
    65461 tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg gcgcaaccac gatggatcag
    65521 ctgaaaacta acatcgaaag tttgcatctg gagttaagcg aagacgtatt agctgaaatt
    65581 gaagcggtgc atcaggttta tacttatccg gcaccataag attaacgccg gttaaaaccg
    65641 gcgttaaata ttaatgacgg cgctgccaga tccacagcgc cgttattgcc agcgcaaaca
    65701 gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc taccattacc gccagcgagt
    65761 aaatgcccag catcaacaac atggcgctgt tttcgccaag gttttgtact gcaatcgcat
    65821 tccccgcccc gacgcttttt ttaccccgct cctgtagcaa cgcattgagc ggaacgacaa
    65881 aaaagccccc catcacgcca atcagcatca acaaggcata ggctggcagc agctcgtgtt
    65941 gcagggaaaa aatcagtacc accacgccaa tcaaaatccc ggctggcata cagcgtgaca
    66001 cggtttccag cgtaactaac ttcgctgccg cacctgcgcc aaccacgata ccaatcgcta
    66061 ccatcgcgtt gagataggtg ggcgtagcgt tatcggtaat gcccagcgcc accggtaccc
    66121 acagcaccaa caggaaacgc agcgtgacac ccgctcccca gaataaactg gtgcccacca
    66181 gcgaaaaacg cgtttcacca ttgcgccata gcgaggtgca ggcattcagg aaactgcggg
    66241 tcatgttgat gagattccag gactgccccg gacgcgccgc cgccagtttg ggaatgtaga
    66301 tattggcaac gaccgcgcca ccgtaggcca gtgcgcatgc ggccagggcg acgaggacat
    66361 gccagtcagc cagcacacca ccggctacgg aaccgagcaa aatcgccgct atggtagaag
    66421 cttccattaa accgttagct ttcactaact tactacccgt ggttaattcg ccgagaatac
    66481 cgtatttcgc cggtgaatag gctgcagcac caacacccac cagcgtatag ccgagaaacg
    66541 gattgatacc aaagcagata ctggctgcgc ccagcagctt caggccgttg gcaaacatca
    66601 tcacccggcc tttggcgaag ctatccgcca cctgcccgac aaacggcgca aaaagaatgt
    66661 aagcacctac aaacaccatt tgcaggatgg gctggctcca ctccggatag aactgcgctt
    66721 tcagtaacgc cagagtggca aacagtaggg cattatcgcc aaacgcagag agaaactgcg
    66781 ccacgataac cgctttcatc cccttcgacc acaacgaagt gttagtgtgc actgactcac
    66841 tcatcgtgtt gttccgcttc gtctacccag cttttcaacg tgacaaagtc aggtttgccg
    66901 ctgccaagta atggcatctg tttcagatag cgaatatcgc gcggtacagc aagctccggc
    66961 acgccgtgct cgcgggcata ctgttgcaac ttatcgcgcg tcagttcgtt atctgtggtg
    67021 aaaagcacca gtgcctcgcc tttgctggca tcgctcttaa tcgcagtggc atggacttta
    67081 tctggcgaaa caccaagtgc cagttgttcc accatttcca gcgacaccat ttcgcctgca
    67141 attttggcaa agcgttttgc gcggccctga atctgcacaa agccctgctc gtcaaaacgc
    67201 acaatatcgc cagtgtcata ccagccgcgc tccatttcgc cgcgaacatt ctcggcggtg
    67261 ggcacttcca gtacacctgg cttctccacc cgcagatagc cgttcattat gttcggccct
    67321 ttcagttgca ggcgtccgcc ctcttcgata ccaggaaccg acaacaggcg cgcatccatt
    67381 cctggtagaa tacgccctac cgtaccgggt ttcgccgcca tcggtacgtt gatagaaacg
    67441 acaggcgcgc actcggttac accatagcct tcaaggatgc gcaggccaaa tttatcctgc
    67501 caaagctgtt tggtactttc ttgtagtttt tctgcgcctg ccaccacata gcgtagacga
    67561 tagaagtcat acgggttggc gaaacgcgcg tagtgaccga ggaaagtcga ggtaccgaac
    67621 aacacggtgc aactgcggtc atacaccaac tccggcacaa tgcggtaatg cagcgggctt
    67681 ggataaagga acacttctgc acctgtaagc agtggcgtaa acaggcctac cgtcagccca
    67741 aaggagtgaa acagcggtaa tgccgacata aagcgatcgt tggtggtgaa gtcggcaatc
    67801 gttttaatct gctcgacgtt cgccagaatg cttttatggc tatggacgac gccttttgga
    67861 tggccttcag aaccggaggt aaaaaggatc agcgcctctt cttccggctg ctgtttaacc
    67921 tgtgccagac gcggcatcag caaatgagcg aagatccata ctttgtcggc agtggtgaca
    67981 tctgctttta aatcttccag atagacccag cgcacctgag taagttgctc cggcagatgc
    68041 cagagtttgc ctttatcgag aaactggcgg gaagtgaaga tggttttgat ttcagccgcc
    68101 gtaatagcac tggtcagccc tttcaccccg gcagtgtagt tcatcattgc gggaatgcgg
    68161 cgacgggcga tggccccaaa aatcactgcc gcactgatgc ctgcattggg cagcattaag
    68221 ccgatgcgtt cgccttcaac actgtatttt tcaaggatgc gtccaacaaa cagcgttttc
    68281 gtaagcaatt tgcgatagga gtctggggta aagttgacgt cttcgacaca tttcttcccg
    68341 gctccgaagc ggtacattgc actcagtaaa gattcgtaca gcgtttcacg cgggcgcacc
    68401 gccattcgcg cttccatcat tatttgatgc agcatttcgc cagcgatttt gcgacggtca
    68461 cgggcacgcg gcgcatccgg catcgccacc tgcgttggtg gcaaaatatg cagagtaatt
    68521 tgcgggaaca agcgacgttt aaccagacct ttcaggcggc tgaagtgcgt aagttccgcc
    68581 ccttcaatac gcacaggaat aaccgttgca ccagacttcg ccgcgacaaa acccgcgcca
    68641 tcgtagattt tcatcagcga gcctgtagtg gtgatgcgtc cttcagggaa aatcaccact
    68701 ggtcggccct gttccaccag acgtaccaga tgtttaatag ccataggttg cgtcgggtcg
    68761 agaggaacaa agtcgataaa tgatttcagc caacgcatat accactgttg gcttattgag
    68821 gtgtaaacgg caaacactgg acgcacaggt aaaaacagtc caagcaaaat gccatcaata
    68881 aaagagacgt gattaggcgt aattagaacg cgctcgccct tcagtgcctg ggtgtcaccc
    68941 gtaacgcgaa cgcgatacaa aacacggcac aaatttcgaa aaaagctaaa aagcattcca
    69001 actccctttg ctctgattca gtaaaagcga atggagggag attacacgag ataaagaacg
    69061 cgagcgacag taaattaggt gcgaaaaaaa acctgcgcat ccgcgcaggt tggtgcaaga
    69121 gacagggtac gaagagcgta ccgaataatc tcaccaatca atacctctgg gatcttgatt
    69181 gtggtctgca cgacgactct tcgccagcga gaaaacgcaa aggaatgaag ggaaatgcaa
    69241 cgaggtgtgt aaattgtcgg ttactgttac agattgatga ccggcaaaaa aaaacctgcg
    69301 catctgcgca ggctggtgta attcatgtgc tcaacccgaa gttgacttca cctatcaata
    69361 cctctgggat caccacttta gcaacctgaa gccaaacgcc accagcggtc aatcgcaaca
    69421 gcgtttcgca aagtgtaacc aaaggtttgc aatctctttt tttgtcttac ttatttgcgt
    69481 ttggctcaca ttcccacgat gaaaacacgc caccccttga accaacgggc gttttccgta
    69541 acactgaaag aatgtaagcg tttacccact aaggtatttt catggcgacc ataaaggatg
    69601 tagcccgact ggcaggcgtt tcagtcgcca ccgtttcccg cgtcattaat aattcaccta
    69661 aagccagtga agcttcccgg ctggctgtgc atagtgcaat ggagtctctt agctatcacc
    69721 cgaacgccaa cgcccgtgcg ctggcgcagc agaccactga aacggtcggt ctgatcgttg
    69781 gtgatgtttc cgatccgttt ttcggtgcaa tggtgaaagc ggtcgaacag gtggcttatc
    69841 acaccggtaa tttcttattg attggcaacg gttaccacaa cgaacaaaaa gagcgtcagg
    69901 ctattgagca actgatccgc catcgctgtg ctgcgttggt cgtccatgcc aaaatgatcc
    69961 cggatgccga tttagcctca ttaatgaaac aaatgcccgg tatggtgctg atcaaccgta
    70021 tcctgcctgg ctttgaaaac cgttgtattg ctcttgacga tcgttacggt gcctggctgg
    70081 caacgcgtca tttaattcag caaggtcata cccgcattgg ttatctgtgc tctaaccact
    70141 ctatttctga cgccgaagat cgtctgcaag ggtattacga tgcccttgct gaaagtggta
    70201 ttgcggccaa tgaccggctg gtgacatttg gcgaaccaga cgaaagcggc ggcgaacagg
    70261 caatgaccga gcttttggga cgaggaagaa atttcactgc ggtagcctgt tataacgatt
    70321 caatggcggc gggtgcgatg ggcgttctca atgataatgg tattgatgta ccgggtgaga
    70381 tttcgttaat tggctttgat gatgtgctgg tgtcacgcta tgtgcgtccg cgcctgacca
    70441 ccgtgcgtta cccaatcgtg acgatggcga cccaggctgc cgaactggct ttggcgctgg
    70501 cggataatcg ccctctcccg gaaatcacta atgtctttag tccgacgctg gtacgtcgtc
    70561 attcagtgtc aactccgtcg ctggaggcaa gtcatcatgc aaccagcgac taaccgcagt
    70621 taaagcaatt ccagcgccag taattcttcg atggtctggc gacggcgaat caaccgcgcc
    70681 tgaccattat caaacagaac ttctggtaac agcggacggc tattgtagtt ggatgacatt
    70741 gatgcgccat atgcccctgt atcatgcagt accagataat cacctgcctt cacttccggc
    70801 aaggcgcggg tttcaacatt tcccccttcc tgctgggtaa agacatcgcc cgattcacat
    70861 aacggtccgg cgacgacggt ttccaccgtt ggcgcgtgtt ccagagaacg accatcagtt
    70921 gccagggcac tgatatggtg gtaactaccg tacattgccg ggcgcatcag atcgttgaac
    70981 ccggcatcaa ccagcacaaa gtggcggctc cccatttgtt tgacgctccg cacctgagta
    71041 attaatacgc cagactgcgc taccaggaag cgacccggtt caatttccag tttcacaggg
    71101 tggcccaaat ggcgggcgat ttgctcacgc gcggcattcc acagaccata ataatgttcg
    71161 gtatcaaccg cctcttcacc ctgttgataa ggaacagaaa gcccaccgcc cgcagaaata
    71221 gcctgtaaat cctgaccgaa ttcgatgacc tgacgcacca tagcaccaca cacctgttcc
    71281 agatgggcat aatcaacgcc agaaccaatg tgcatgtgaa tgccgaccag ctgcagatga
    71341 tgacgttgta tcacgtccag tgcggcgggc agatcggtgt accagatacc gtgcttgctg
    71401 ttttcgccac cggtattggt tttttggcta tgtccgtgac caaaccccgg attaacgcgc
    71461 agccataccc gatgccctgg cgaaacctgg cccagttggt cgagcatatc aacagaaccc
    71521 gcattcaccg gaatttgcaa ttcactgacg cgttcaagcg tcgcctgatc gataacatct
    71581 gccgtaaaaa caatatcatc ggggtgcgtt tgcggattgt aacccgccgc caacgcacgc
    71641 tctatttcgc ctaacgagac ggaatccact ttcacgccct gctcacgcat taagcgcaaa
    71701 atatgaatat tggaacaggc tttctgtgca aagcgcacca catcaaactg tttcagcgct
    71761 gcaatctgcc gacgaataat ttgcgcatcg tagacccaca ccgggcagcc aaattcagcg
    71821 ggcaaacgca gcagattttc ggcggtgaga tcggtatcgg tgctgaacag tgaatgtggc
    71881 ataacaaact ccagataagt gcttttttat gattacgcca catcataaaa agaataaaaa
    71941 atatcgattt atgtcgagtc tatgcaaaaa tgatatggat taccggattg cgagagagcg
    72001 ctaatggccg ccgttaactt acgtcatatt gaaatttttc atgcggtaat gaccgccgga
    72061 agcctgactg aggcggcaca cctgctacac acctcacagc caaccgtcag ccgcgaactt
    72121 gcgcgctttg agaaggtgat cgggctgaaa ttgtttgagc gcgtacgtgg gcgattacat
    72181 cctaccgtgc aaggactgcg tctgtttgaa gaagtgcaac gatcctggta cggactggat
    72241 cgcattgtca gcgccgcaga aagtctgcgc gagtttcgcc agggagaact gtctattgcc
    72301 tgcctgccgg tcttttcgca atctttttta ccgcagctcc tgcaaccctt tctggcacgt
    72361 tatcccgatg tcagcttaaa tatcgtgccc caggaatcac cgctacttga agagtggctc
    72421 tcggcccagc gtcatgattt aggactcact gaaacgctcc atacgcctgc gggaacagaa
    72481 cgtaccgaat tactctcttt agatgaagtg tgtgtgttac ctccgggtca tccgctggcg
    72541 gtaaaaaagg tattaacgcc ggatgatttt cagggtgaga actacatcag cctttcccgt
    72601 actgacagct atcgccagtt gctggatcag ctatttactg aacatcaggt taaacgacgc
    72661 atgatcgtag aaacccacag cgccgcgtca gtctgcgcaa tggtacgggc gggggtaggt
    72721 gtttcggtgg ttaacccgct caccgcacta gattatgcgg caagcggttt agtggtgcgg
    72781 cggttcagta ttgcggttcc gttcaccgtc agcctgatcc gccccctgca ccgcccgtca
    72841 tcagcgctgg ttcaggcgtt tagtgggcat ttacaagcgg ggttaccgaa actggtcact
    72901 tctcttgacg ctattttgtc gtcagctacg acagcataaa agcgacagca tcctcggcat
    72961 ggatcgccgc ggtatcaaac acaggcaaaa cactgcgctc ttctggcacc agtaaaccaa
    73021 tttctgtgca gccaaaaatg acgccctgtg cgccctgttc tgcaaggcga gcaatcactt
    73081 gcgcataata agcgcgtgac gcttcggtaa attgccccag acacagttct tcaaaaataa
    73141 tctgattaat tttcgcccgt tcatccgctt caggaataag acaattgatg gaaaattgtt
    73201 ccgtcagccg cccgcgataa aaatcctgtt ccatggtgta acgcgtaccc agcagcgcca
    73261 cacgagtcat tccggccccg gtaattgcac gtccggtggc atccgcaatg tgtaagaaag
    73321 gcagagtgca acgtgactca atggcatccg ccactttatg catcgtattg gtacataaca
    73381 caataccttc tgcgcccgcc cgctgtaagc caagcgccgc ctcagccaga atgtccccgg
    73441 ttttatccca ttccccgcga cgctggcact cttctatttc atgaaaatcg acgctatgta
    73501 gcagcacttg cgcagagtga agcccaccaa gccgctgttt aatgccttca tttatcaaac
    73561 gatagtaagg aatggtggat tcccagctca ttcctcccag caaaccaatt gttttcattc
    73621 ctctctcctg atgtgcgtta ccgcaacaag gctgaataag gaacgagaca aacgcctcaa
    73681 cggccaagtg cccgatctct attaacgaaa aaagggccgg atgtacaaca catccggccc
    73741 gtgaaatcag atgccgatat ttctcaactt ctcgcctgcc atcagtttgc gttcgatatg
    73801 ttccagcgtg acatttttgg tttccggaat gagccagaaa gtgatgccca caaacgcaat
    73861 gttcagcgca gtgtagagcc agaacgtacc ggcagcgcca atgctgtcga gcagcgtcag
    73921 gaaggtcgcg ccgataatca tattcgacac ccagttcgtg gtggtcgaac aggtaatacc
    73981 gaaatcgcgg catttcagcg gctgaatttc agagcacagg atccacacca ctggcgcggc
    74041 gctcatcgca taaccggcaa tacacatcat cgtcatgcca acagagagcc aggacaagcc
    74101 actggaagcc gtaccgttat caaactgcat caggcaatag cccagcacca gagtgcctaa
    74161 cgccatcacg ctgaaaccaa ttttcagagc cggtttacgc cctgctttat ctaccgtaaa
    74221 caccgcaata aaggtggcga acataaaggt cagccctacg accagagtcg caatcatctg
    74281 ttgttctgtg gtcgtaaagc ccgccatttt gaagatacgc ggcgcgtagt acatgatgat
    74341 gttcataccg gtaaactgct gcatcgcctg caacaacata ccgagaaaca cagcacgacg
    74401 gacgttacgg ttgatcttaa acagtgccca accgccctgt tttaacttca ggctttcacg
    74461 aatttcgttg agttcttctc gcgctttttc cgacgtatcg cgcagcatac gcaatacttc
    74521 ttccgcctca atatgacgcc ccttttccgc cagccagcgc gggctatttg gcaggaagac
    74581 taccagaata atcagcagaa ctgctggtaa agcaagaacc cccaacattg cgcgccagtt
    74641 accgctataa ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat
    74701 caactggtac atactgatca tcttaccgcg aacgttttca cttgccattt cagaaagata
    74761 cagaggagcg gtgtaagacg cgatcccgac agcaatgccc agcaccacac gagcggcgat
    74821 taacatctct acgctggtcg caaaagcgga ccctatagaa ccgagtacaa acaggatggc
    74881 ccccgccatc aggctgtatt tacgccccag gcggaacgac agccaaccat taaacagcgc
    74941 accaattgct gcaccgagca tcatgctact aaccacccat tcctgcaaac gactggtcag
    75001 cacaaagtga tcggtaatga acggcaacgc tccggcgatt acgccgatat caagaccaaa
    75061 taacaatcct gcgaccgcag cagctaccga aacaaacata ttcatacgcc gcgtatcccg
    75121 caaagaacgt ggcgttaaag cagattccgt attgatagta accatttttt cctgccagca
    75181 gagagtaaga catagtgaaa aaatacgtga acaactcacg caggtgtcag gtcggaaaca
    75241 gcataaatat ggattaaatt gctgcgacat gtcgttatgt gatggatatt ccaattttca
    75301 aa
//
LOCUS       DAJOSY010000009        70301 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJOSY010000009 DAJOSY010000000
VERSION     DAJOSY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.009"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(125..3274)
                     /gene="acrB"
                     /locus_tag="OHN27_000655"
     CDS             complement(125..3274)
                     /gene="acrB"
                     /locus_tag="OHN27_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HCQ2285153.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGDSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3297..4490)
                     /gene="acrA"
                     /locus_tag="OHN27_000656"
     CDS             complement(3297..4490)
                     /gene="acrA"
                     /locus_tag="OHN27_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCQ2285154.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4632..5279
                     /gene="acrR"
                     /locus_tag="OHN27_000657"
     CDS             4632..5279
                     /gene="acrR"
                     /locus_tag="OHN27_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCQ2285155.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5407..8769
                     /gene="mscK"
                     /locus_tag="OHN27_000658"
     CDS             5407..8769
                     /gene="mscK"
                     /locus_tag="OHN27_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCQ2285156.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWQKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLAGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8981..9142)
                     /gene="rsmS"
                     /locus_tag="OHN27_000659"
     CDS             complement(8981..9142)
                     /gene="rsmS"
                     /locus_tag="OHN27_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCQ2285157.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPAHTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9156..9683)
                     /gene="priC"
                     /locus_tag="OHN27_000660"
     CDS             complement(9156..9683)
                     /gene="priC"
                     /locus_tag="OHN27_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCQ2285158.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLVRVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9753..10130
                     /locus_tag="OHN27_000661"
     CDS             9753..10130
                     /locus_tag="OHN27_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCQ2285159.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10283..10834
                     /gene="apt"
                     /locus_tag="OHN27_000662"
     CDS             10283..10834
                     /gene="apt"
                     /locus_tag="OHN27_000662"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCQ2285160.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10963..12894
                     /gene="dnaX"
                     /locus_tag="OHN27_000663"
     CDS             10963..12894
                     /gene="dnaX"
                     /locus_tag="OHN27_000663"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCQ2285161.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12227..12291
                     /gene="dnaX"
                     /locus_tag="OHN27_000663"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12947..13276
                     /locus_tag="OHN27_000664"
     CDS             12947..13276
                     /locus_tag="OHN27_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCQ2285162.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13276..13881
                     /gene="recR"
                     /locus_tag="OHN27_000665"
     CDS             13276..13881
                     /gene="recR"
                     /locus_tag="OHN27_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCQ2285163.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13991..15865
                     /gene="htpG"
                     /locus_tag="OHN27_000666"
     CDS             13991..15865
                     /gene="htpG"
                     /locus_tag="OHN27_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCQ2285164.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16046..16690
                     /gene="adk"
                     /locus_tag="OHN27_000667"
     CDS             16046..16690
                     /gene="adk"
                     /locus_tag="OHN27_000667"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCQ2285165.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16926..17888
                     /gene="hemH"
                     /locus_tag="OHN27_000668"
     CDS             16926..17888
                     /gene="hemH"
                     /locus_tag="OHN27_000668"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCQ2285166.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17885..18844)
                     /gene="aes"
                     /locus_tag="OHN27_000669"
     CDS             complement(17885..18844)
                     /gene="aes"
                     /locus_tag="OHN27_000669"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCQ2285167.1"
                     /translation="MKPENKLPVLDLISAGMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQVEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18996..20300
                     /gene="gsk"
                     /locus_tag="OHN27_000670"
     CDS             18996..20300
                     /gene="gsk"
                     /locus_tag="OHN27_000670"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCQ2285168.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20433..22109)
                     /locus_tag="OHN27_000671"
     CDS             complement(20433..22109)
                     /locus_tag="OHN27_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCQ2285169.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGVAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22346..23566)
                     /gene="fsr"
                     /locus_tag="OHN27_000672"
     CDS             complement(22346..23566)
                     /gene="fsr"
                     /locus_tag="OHN27_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCQ2285170.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23784..25436
                     /gene="ushA"
                     /locus_tag="OHN27_000673"
     CDS             23784..25436
                     /gene="ushA"
                     /locus_tag="OHN27_000673"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCQ2285171.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25473..25952)
                     /gene="ybaK"
                     /locus_tag="OHN27_000674"
     CDS             complement(25473..25952)
                     /gene="ybaK"
                     /locus_tag="OHN27_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCQ2285172.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGALAKILDAKFADIARRD"
     gene            complement(26156..26950)
                     /locus_tag="OHN27_000675"
     CDS             complement(26156..26950)
                     /locus_tag="OHN27_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCQ2285173.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27088..27429
                     /locus_tag="OHN27_000676"
     CDS             27088..27429
                     /locus_tag="OHN27_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCQ2285174.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27745..30249)
                     /gene="copA"
                     /locus_tag="OHN27_000677"
     CDS             complement(27745..30249)
                     /gene="copA"
                     /locus_tag="OHN27_000677"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCQ2285175.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30511..31443
                     /gene="glsA"
                     /locus_tag="OHN27_000678"
     CDS             30511..31443
                     /gene="glsA"
                     /locus_tag="OHN27_000678"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCQ2285176.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31446..32738
                     /locus_tag="OHN27_000679"
     CDS             31446..32738
                     /locus_tag="OHN27_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2285177.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            32863..33270
                     /gene="cueR"
                     /locus_tag="OHN27_000680"
     CDS             32863..33270
                     /gene="cueR"
                     /locus_tag="OHN27_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCQ2285178.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33271..33729)
                     /locus_tag="OHN27_000681"
     CDS             complement(33271..33729)
                     /locus_tag="OHN27_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCQ2285179.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33726..34643)
                     /locus_tag="OHN27_000682"
     CDS             complement(33726..34643)
                     /locus_tag="OHN27_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCQ2285180.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34789..35466
                     /gene="fetA"
                     /locus_tag="OHN27_000683"
     CDS             34789..35466
                     /gene="fetA"
                     /locus_tag="OHN27_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCQ2285181.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35453..36232
                     /gene="fetB"
                     /locus_tag="OHN27_000684"
     CDS             35453..36232
                     /gene="fetB"
                     /locus_tag="OHN27_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCQ2285182.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36295..37149)
                     /gene="cnoX"
                     /locus_tag="OHN27_000685"
     CDS             complement(36295..37149)
                     /gene="cnoX"
                     /locus_tag="OHN27_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCQ2285183.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQESNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(37210..38019)
                     /gene="ybbO"
                     /locus_tag="OHN27_000686"
     CDS             complement(37210..38019)
                     /gene="ybbO"
                     /locus_tag="OHN27_000686"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCQ2285184.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(38009..38632)
                     /gene="tesA"
                     /locus_tag="OHN27_000687"
     CDS             complement(38009..38632)
                     /gene="tesA"
                     /locus_tag="OHN27_000687"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCQ2285185.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMPA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            38603..39289
                     /locus_tag="OHN27_000688"
     CDS             38603..39289
                     /locus_tag="OHN27_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2285186.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39286..41700
                     /locus_tag="OHN27_000689"
     CDS             39286..41700
                     /locus_tag="OHN27_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2285187.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRITLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(42413..43507)
                     /gene="mnmH"
                     /locus_tag="OHN27_000690"
     CDS             complement(42413..43507)
                     /gene="mnmH"
                     /locus_tag="OHN27_000690"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCQ2285188.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(43576..44502)
                     /gene="allS"
                     /locus_tag="OHN27_000691"
     CDS             complement(43576..44502)
                     /gene="allS"
                     /locus_tag="OHN27_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCQ2285189.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            44732..45214
                     /gene="allA"
                     /locus_tag="OHN27_000692"
     CDS             44732..45214
                     /gene="allA"
                     /locus_tag="OHN27_000692"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCQ2285190.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            45292..46107
                     /gene="allR"
                     /locus_tag="OHN27_000693"
     CDS             45292..46107
                     /gene="allR"
                     /locus_tag="OHN27_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCQ2285191.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            46197..47978
                     /gene="gcl"
                     /locus_tag="OHN27_000694"
     CDS             46197..47978
                     /gene="gcl"
                     /locus_tag="OHN27_000694"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCQ2285192.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRAMAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAIEMLLQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            47991..48767
                     /gene="hyi"
                     /locus_tag="OHN27_000695"
     CDS             47991..48767
                     /gene="hyi"
                     /locus_tag="OHN27_000695"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCQ2285193.1"
                     /translation="MLRFSANLSMLFGEYDFFARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            48867..49745
                     /gene="glxR"
                     /locus_tag="OHN27_000696"
     CDS             48867..49745
                     /gene="glxR"
                     /locus_tag="OHN27_000696"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCQ2285194.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            49915..51369
                     /locus_tag="OHN27_000697"
     CDS             49915..51369
                     /locus_tag="OHN27_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HCQ2285195.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAAGDFKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTAAEKTGEQKVTG"
     gene            51430..52791
                     /gene="allB"
                     /locus_tag="OHN27_000698"
     CDS             51430..52791
                     /gene="allB"
                     /locus_tag="OHN27_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HCQ2285196.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKSEGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHICHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            52848..54149
                     /locus_tag="OHN27_000699"
     CDS             52848..54149
                     /locus_tag="OHN27_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCQ2285197.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            54171..55316
                     /gene="glxK"
                     /locus_tag="OHN27_000700"
     CDS             54171..55316
                     /gene="glxK"
                     /locus_tag="OHN27_000700"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HCQ2285198.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(55544..56329)
                     /gene="allE"
                     /locus_tag="OHN27_000701"
     CDS             complement(55544..56329)
                     /gene="allE"
                     /locus_tag="OHN27_000701"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCQ2285199.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNS
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(56340..57575)
                     /gene="allC"
                     /locus_tag="OHN27_000702"
     CDS             complement(56340..57575)
                     /gene="allC"
                     /locus_tag="OHN27_000702"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCQ2285200.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAILRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(57597..58646)
                     /gene="allD"
                     /locus_tag="OHN27_000703"
     CDS             complement(57597..58646)
                     /gene="allD"
                     /locus_tag="OHN27_000703"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HCQ2285201.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAAHAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            58963..60630
                     /locus_tag="OHN27_000704"
     CDS             58963..60630
                     /locus_tag="OHN27_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCQ2285202.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGAGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            60640..61899
                     /locus_tag="OHN27_000705"
     CDS             60640..61899
                     /locus_tag="OHN27_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCQ2285203.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            61910..62725
                     /locus_tag="OHN27_000706"
     CDS             61910..62725
                     /locus_tag="OHN27_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCQ2285204.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWMLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            62722..63615
                     /locus_tag="OHN27_000707"
     CDS             62722..63615
                     /locus_tag="OHN27_000707"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCQ2285205.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(63820..64887)
                     /gene="purK"
                     /locus_tag="OHN27_000708"
     CDS             complement(63820..64887)
                     /gene="purK"
                     /locus_tag="OHN27_000708"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCQ2285206.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFG"
     gene            complement(64884..65393)
                     /gene="purE"
                     /locus_tag="OHN27_000709"
     CDS             complement(64884..65393)
                     /gene="purE"
                     /locus_tag="OHN27_000709"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCQ2285207.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(65511..66233)
                     /gene="lpxH"
                     /locus_tag="OHN27_000710"
     CDS             complement(65511..66233)
                     /gene="lpxH"
                     /locus_tag="OHN27_000710"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCQ2285208.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(66236..66730)
                     /gene="ppiB"
                     /locus_tag="OHN27_000711"
     CDS             complement(66236..66730)
                     /gene="ppiB"
                     /locus_tag="OHN27_000711"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCQ2285209.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            66904..68289
                     /gene="cysS"
                     /locus_tag="OHN27_000712"
     CDS             66904..68289
                     /gene="cysS"
                     /locus_tag="OHN27_000712"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCQ2285210.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(68325..68846)
                     /locus_tag="OHN27_000713"
     CDS             complement(68325..68846)
                     /locus_tag="OHN27_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCQ2285211.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(68954..69166)
                     /gene="ybcJ"
                     /locus_tag="OHN27_000714"
     CDS             complement(68954..69166)
                     /gene="ybcJ"
                     /locus_tag="OHN27_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCQ2285212.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(69168..70034)
                     /gene="folD"
                     /locus_tag="OHN27_000715"
     CDS             complement(69168..70034)
                     /gene="folD"
                     /locus_tag="OHN27_000715"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCQ2285213.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
BASE COUNT        16583 a        18145 c        18867 g        16706 t
ORIGIN      
        1 ttttatccgt ggttaatact ggttttcgta tgagatcctg agttggtggt tcaattactc
       61 cttaatgttc gtaggttatg cataaaaaag gccgcttgcg cggccttagt gattacacgt
      121 tgtatcaatg atgatcgaca gtatggctgt gctcgatatc ttcattcttg cggctaaagc
      181 ggcggcgaac caccacaaag aataccggaa cgaagaagat tgccagtacc gttgcggtca
      241 ccatcccgcc cattacaccg gtacctactg cgttctgcgc gccggaacca gcaccagtac
      301 tgataaccag cggcataacg ccgaggataa acgccagcga ggtcatcagg atcggacgta
      361 aacgcatccg caccgcatca agcgtcgctt caatcagacc tttaccttct ttatccatca
      421 agtctttggc gaattcgacg ataagtatcg cgttcttcgc cgacaaccca atggttgtga
      481 gcaggcctac ctggaagtaa acgtcattgg tcaggccacg gaaggtggca gccagcaacg
      541 caccgataac ccccagcgga acgaccagca taacggagaa cggaatcgac cagctctcgt
      601 acagcgccgc cagacacagg aacacgacaa tcaacgaaat cgcgtacagt gaaggtgcct
      661 ggttgccgga gagacgttcc tgataggaca tccccgtcca gtcatagcca acaccggtag
      721 gcagtttgct cgccagttgt tccatcagct ccattgcttc accggtactt ttacccggtg
      781 ccgcctggcc taagatttcc atggatggca ggccgttgta acgttccaga cgcggcgaac
      841 cgtactccca acgagaagag gagaacgccg agaatggcac catctgacca tcagcagcac
      901 gaacatacca gtcgccgata tcatccggca gcatacggta tttcgcttct gacatgacgt
      961 aaactttctt cacacgaccg cggtcgataa agtcgttcac atagctgccg ccccatgcag
     1021 cgcccagagt ggtgttaatg tcgttgatag aaacacccag cgcctgcgct ttttcctggt
     1081 cgatatcaat cttaaactgc ggggtatctt ccagaccgtt tggacgtacg ctggtcaaca
     1141 tatcagggtg cttcgctgct tctgcaagca gctggttacg cgcctgagtc agtttttcgt
     1201 gaccaaggcc agcctggtca atcagctcaa agtcaaagcc ggttgcggta cccagttcca
     1261 cgattgcggg caggttaaag gcgaaaacca tcgcatcttt gatttgcgag aaagcgcgtg
     1321 ttgcacgcat ggtaatcgct tcaactttgt tttcttcgcc cggacgatcg gcccagtcct
     1381 tcaaggaaac gaacgcaata ccggtattct gaccacgtcc cgcaaagccg aagccgttaa
     1441 cggcgaacac cgactcaacg ttgttctttt ctttggtcag atagtaatgc gttacctcat
     1501 tgagcacttt ctgtgtacgt tcctgcgttg cacctgctgg cagctgaacc atggtcataa
     1561 atacgccctg gtcctcatct ggcaagaagg agcttggcag acgcacaaac agataggcca
     1621 tgccgaccac gatgatcaga tacagcacca ggtaacgccc tgtactgcgc agaataccgc
     1681 ctacgctgtc ggtgtagtgg tgcgtgctct tctcgaacat gcggttaaac cagccgaaga
     1741 agcctttttt accttccccg tgatcgcctt tggcaatcgg tttcagcatg gtggcacaaa
     1801 gagccggagt caggatcaac gctaccagta ccgacagcgc cattgctgaa acaatggtaa
     1861 tagagaactg acgatagata gcaccagtag aatcgccaaa gaaggccatc ggtacgaata
     1921 ccgccgacag taccatcgcg ataccgacca gagcgccctg aatctgcccc atcgacttac
     1981 gggtagcttc ttttggcggc aaaccttctt ccgccataac acgctcaacg ttttctacca
     2041 caacgatagc gtcatccacc aacaagccga tggcgagcac catcccgaac attgttagcg
     2101 tgtttatcga gaagccaaag gcggcaagga cggcaaaggt cccgagcaat accaccggta
     2161 cggcaatggt cggaatcaac gtcgcgcgga agttctgcag gaacagatac ataaccagga
     2221 acacgaggat gatcgcttcg accagcgttt taaccacttc gtgaatagag attttcacga
     2281 acggcgtggt gtcgtacggg taaacaattt tcagacctga cgggaagaac ggttccatct
     2341 tcgccagttc agcacggatt gccgcagcgg tatccagcgc gtttgcaccg gtcgccagct
     2401 tgatccccag accggaagcc ggttggccgt taaactctgc gatgatgtcg tagttctcac
     2461 cacccagctc aatcttcgcg acgtcacgca gcagcacgcg ggaaccatcc tgattcactt
     2521 tcagcaggat tttgccgaac tcttcagtag aggtcagacg cgtctgagca ataatagagg
     2581 cgttaagctg ttgccctttc accggcggcg taccaccgag ctgacccgcc gcaacctggg
     2641 cgttctgcgc tttgatggcg gtaatgacat caaccggcgt tagctggaat ttgttcagct
     2701 cattcgggtt catccagata cgcatcgcgt actgtgaacc gaacaactga acatcaccca
     2761 cgcccgacgt acggctgatg gcatctttca tattcgccgc tacgtagtca gagatatcct
     2821 cctgcgtcat ggtgccatcg gtgttgataa cgccgacaac catcaggaag ctgctggatg
     2881 atttctcaac gctcacccct tgctgctgaa cttcttgcgg cagcaacggc atcgccagct
     2941 gcagtttgtt ctgcacctga acctgcgcga tatccgcatc agtaccagac tcaaaggtca
     3001 gggtgatctg cacggtaccc gtggagtcac tgttagagga catgtacatc aggttatcga
     3061 taccgttcat attctgttcg ataacctgtg tcaccgtgtc ctgcactgtt ttcgcatcag
     3121 cgccggggta ggaggcggag atcgttactg ccggcggtgc aatcgtagga tattgcgcca
     3181 ccggcagttt gaggatcgcc agcccccctg ccaacatgat gataatggcg atcacccacg
     3241 caaaaatcgg gcgatcgata aagaaattag gcatgtctta acggctcctg tttaagttaa
     3301 gacttggact gttcaggctg agcaccgctt gcggcttgct ggttattatc agcggtaact
     3361 tcttgtgctt ttacctggac accaggacgc actttctgca gcccacttat tactacgcga
     3421 tcgcctgctt tcagaccttc tgtcaccagc cacttatcgc caatagcctg gcttgcaacg
     3481 atcggacggg tttccacttt gtcatccgcg ccaactacca gtacggtggc atcaccacgc
     3541 ggcgtacggg ttacgccctg ttgcgggact aaaatagcgt ttggattaag cccttcttcc
     3601 agacgtgcgc gcacgaacat acccggcagc agagtgtgat ccgggttcgg gaagatagcg
     3661 cgtagggtga tagacccagt ggtctgatca acggtaacgt cagagaattc cagcgtaccg
     3721 tcctgcggga acttaatgcc gtcactggtg atcagcgaca ctttggcttt gccgttctct
     3781 tgtttcagcg tgccattcgc cagttcctgt ttcaggcgca ggaagtcgtt gctggactgg
     3841 gtcacatcaa cgtagatcgg atcaagttgc tgcacggttg ccagcgcagt cgcctgaccg
     3901 ttctgtacca atgcgccttc cgtcacgttc gacttaccaa tgcgaccgct aatcggagag
     3961 gtgactttgg tgtaagccag attgattcgc gcagtttcaa cggcagcttt cgccgcagtt
     4021 accgcagcat tcgcctgttg cgcatcagcc agagcctgat cgtactcttg cttactgatg
     4081 tactgagtac cgagcagttt ctgataacga ttcaccgtca attgcgcgat attggctgca
     4141 gcctgggctt tcgccagatc acctttcgca ctgtcgtatg tcgcctgata ggtcgcagga
     4201 tcaatctgat agagagagac acctgcttcg atgtcgctac cttctttgaa attacgcttc
     4261 aggataatcc cgctaacttg aggacgaact tctgcgatcc ggtaggcact ggtgcgaccc
     4321 ggaagctcgg ttgtgatctg cagaggttca gttttgactg ttactacgcc aacggcgggc
     4381 atctgctggc caccttgttg ggcctgtttg tcgtcacatc ctgttagggc taagctgcct
     4441 gagagcatca gaacgaccgc cagaggcgta aaccctctgt ttttgttcat atgtaaacct
     4501 cgagtgtccg atttcaaatt ggtcaatggt caaaagttaa taaacccatt gctgcgttta
     4561 tattatcgtc gtgctatggt acatacattc acaaatgtat gtaaatctaa cgcctgtaaa
     4621 ttcacgaaca tatggcacga aaaaccaaac aagaagcgca agaaacgcgc caacacatcc
     4681 tcgatgtggc tctacgtctt ttctcacagc agggggtatc atccacctcg ctgggcgaga
     4741 ttgcaaaagc agctggcgtt acgcgcggtg caatctactg gcattttaaa gacaagtcgg
     4801 atttgttcag tgagatctgg gaactgtcag aatccaatat tggtgaacta gagcttgagt
     4861 atcaggcaaa attccctggc gatccactct cagtattaag agagatatta attcatgttc
     4921 ttgaatccac ggtgacagaa gaacgacgtc gattattgat ggagattata ttccacaaat
     4981 gcgaatttgt cggagaaatg gctgttgtgc aacaggcaca acgtaatctc tgtctggaaa
     5041 gttatgaccg tatagaacaa acgttaaaac attgtattga agcgaaaatg ttgcctgcgg
     5101 atttaatgac gcgtcgggca gcaattatta tgcgcggcta tatttccggc ctgatggaaa
     5161 actggctctt tgccccgcaa tcttttgatc ttaaaaaaga agcccgcgat tacgttgcca
     5221 tcttactgga gatgtatctc ctgtgcccca cgcttcgtaa tcctgccact aacgaataac
     5281 cctgaatctg actccaggat ttttcctgga cattttcgtc gttgctattc tggttcactg
     5341 cgtcgtgata ttcttgcggt ttgacttttt caggtcgttc ttcaggttca gaaaccttca
     5401 ttcatcatga ctatgttcca gtattacaaa cgatcacggc attttgtttt ttcagcattt
     5461 attgcttttg tttttgtctt gttatgccag aacacggcgt ttgcgcgggc gtcatcgaat
     5521 ggtgatctgc cgacaaaagc ggacctgcag gcgcaacttg actcactaaa taaacaaaaa
     5581 gatctttctg ctcaggacaa actggtgcag caggatctga cagatacatt agccaccctc
     5641 gataaaatcg atcgcataaa agaagagaca gttcagctac ggcaaaaagt cgctgaagcg
     5701 ccggaaaaaa tgcgccaggc gaccgcggcg ttaacagcac ttagcgatgt cgataacgac
     5761 gaagaaacgc gcaaaattct gagtacgctt tcgttgcgcc agctggaaac tcgcgttgcc
     5821 caggcgctgg acgatttgca aaacgcacaa aacgatctgg cgtcttataa cagccagctg
     5881 gtttcgttac agacgcagcc cgaacgcgtg caaaatgcga tgtataacgc ttcgcagcag
     5941 ctgcaacaaa ttcgcagtcg tctggatggg actgatgtcg gcgagacagc cttacgtccc
     6001 agccagaaag tgttaatgca ggcccagcag gcgttgctga atgcggagat tgaccagcag
     6061 cgtaaaagcc tggaagggaa caccgtcttg caggatacct tgcaaaagca acgtgattac
     6121 gtgacggcga acagcgctcg tctggagcac cagttacaac tgttgcaaga agcggtaaac
     6181 agcaagcgcc tgactttaac cgaaaaaacg gcgcaggaag ccgtctcccc ggatgaagcc
     6241 gcgcgtattc aggctaatcc gctggtgaag caggaactgg aaattaacca gcagttaagt
     6301 cagcgtctga ttaccgcgac tgaaaacggt aatcagttga tgcagcaaaa cattaaagtc
     6361 aaaaactggc tggagcgggc gctgcaatcg gaacgcaata ttaaagagca gattgccgtc
     6421 ctgaagggca gcctgctgtt gtctcgtatc ctttaccagc aacaacaaac gctgccctcg
     6481 gcggatgaac tggaaaacat gaccaaccgc atcgcggatt tgcgtctcga acagtttgaa
     6541 gttaaccagc agcgtgatgc actcttccag agcgatgcgt tcgtcaacaa actggaagaa
     6601 ggtcacacca acgaagtcaa cagcgaagtt cacgatgcgt tattgcaagt ggttgatatg
     6661 cgtcgcgaat tgctggatca actcaacaaa cagttgggta accagctgat gatggccatt
     6721 aacctgcaaa tcaaccagca gcagttaatg agtgtgtcga aaaacctgaa atccatcctg
     6781 actcagcaaa tcttttgggt gaacagtaac cgtccaatgg actgggactg gatcaaagcg
     6841 ttcccgcaaa gcctgaaaga tgaatttaag tcgatgaaaa tcacggtgaa ctggcaaaaa
     6901 gcctggcccg ccgtttttat cgctttcctc gctggtttgc cgctgctgtt gattgccggg
     6961 ctgatccact ggcgtctggg ctggctgaaa gcgtatcaac aaaaactggc ttccgctgtg
     7021 ggttccctgc gtaacgacag ccagctcaac acaccaaaag cgatccttat cgacctgatc
     7081 cgtgcgctgc cggtgtgcct gattattctc gcggttggcc tgattctgtt gaccatgcag
     7141 ctcaacatca gcgaactgct atggtcgttc agtaaaaaac tggcgatttt ctggctggtg
     7201 tttggcctgt gctggaaggt actggagaaa aatggcgttg ccgtgcgtca cttcggcatg
     7261 ccggagcagc agaccagcca ctggcgtcgg caaattgtcc gcatcagtct cgcgttgctg
     7321 cctatccatt tctggtctgt ggtggcagaa ctttccccgc tgcatctgat ggatgatgtg
     7381 ctggggcaag cgatgatttt cttcaacctg ctgctgattg ccttcctggt atggccgatg
     7441 tgccgcgaaa gctggcgtga taaagagtcg cacaccatgc gactggtcac cattaccgtg
     7501 ctgtcgataa tcccgattgc gctgatggtg ctgactgcta caggctactt ctacactacg
     7561 ctgcgtctgg caggacgctg gattgaaacc gtttatctgg tgatcatctg gaacctgctg
     7621 taccagacgg tactgcgtgg cttaagcgta gcggcgcggc gtatcgcctg gcgtcgtgcg
     7681 ctggcgcgtc ggcagaatct ggtgaaagag ggcgcagaag gtgccgaacc gccggaagaa
     7741 cccaccattg cactggaaca ggttaaccag cagacgctgc gtattaccat gttgctgatg
     7801 tttgcgctgt tcggtgtcat gttctgggca atttggtccg atttgatcac cgtgttcagc
     7861 tatctcgaca gcattacgct ctggcattac aacggtactg aagctggtgc tgcggtggtg
     7921 aaaaacgtca ccatgggcag cctgttgttt gcgattatcg cctcaatggt ggcctgggcg
     7981 ttgattcgca acctgcctgg tttgctggaa gtgctggtgc tctcgcgact gaatatgcgc
     8041 cagggcgcgt cgtatgccat tactaccatc cttaactaca tcattattgc cgttggtgcg
     8101 atgacggtgt tcggatcgct gggcgtctct tgggataaac tccagtggct ggccgcagca
     8161 ttatccgtag gtcttggttt tggcttacag gagatcttcg gtaacttcgt ctccggtttg
     8221 atcattctgt tcgaacgtcc ggtgcgtatt ggagatacgg taaccattgg tagcttctcg
     8281 gggacggtaa gtaagatccg tattcgtgcg acaacgatta ccgatttcga tcgcaaagaa
     8341 gtgatcatcc cgaacaaagc gtttgttacc gagcgtctga tcaactggtc gttgactgac
     8401 actactacgc gtctggtgat ccgtctcggc gtggcctatg gctccgatct ggaaaaagtg
     8461 cgtaaagtgt tactgaaggc ggcgactgag cacccaagag tgatgcacga accaatgccg
     8521 gaagtcttct ttacggcgtt tggtgccagc acgttggatc atgagctgcg tctgtatgtg
     8581 cgtgaactgc gtgaccgtag tcgtactgtc gatgagctga accgtactat cgatcagctg
     8641 tgccgtgaaa acgacatcaa cattgccttt aaccagcttg aagtgcatct gcacaacgag
     8701 aagggcgatg aggtgacgga agtaaaacgc gactacaaag gcgacgatcc gacaccagcg
     8761 gttgggtaat aaccaaaggg ggcgacattt agttgccccg agattactga caaagtgcgc
     8821 gttgttcatg ccggatgcgg cgtgaccgcc ttatccggcc tacgaaaccg caagaattca
     8881 atatattgca ggagcggtgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg
     8941 cccacaatct taggggacat ttagcggccc catttatttc tcacttttcc gcctcatcat
     9001 cgcgcgttaa tttcttttca taatcgcgtt ttacaatatc cagcgcgcgc agcacggtgt
     9061 gggctgggat ctgattttct tccagcagca caatcaaatc gacagccagt ttgacatcgt
     9121 caggggcatt ttcaagtgac atactctctc cattgctagc gggttaagcg cgctagcctg
     9181 ttttcgattt tttccagcgc atggcggcag cgtgccaggc gcgcttcata ggcttccact
     9241 tcacgatgca gcgtttgctg ttccacgaga tcggtcaccc gcaccaggcg ggctctgcgt
     9301 tcggcaacca tctcacgtag ccgccgctca aaatcctgat gctgaatacg tttacgctgc
     9361 cagcgggcaa ttttcggtgg cgcactgtcc cactcgcgca atgaccaggc ggaggcttca
     9421 cgcgcgatgg cttccagttg tgccgccaga tgttccgcca gccaggccac ttgcggcagc
     9481 tgttgctgct caactgcatg acgaagcgca gccagattat cgcccgcctc gtcgagacaa
     9541 gcctgtagtg ttgtcgcacg agtctgaaaa agatgcctgt cgaaacgagc acttagcgtg
     9601 gcgaactgtg acaccggggc acaacgctga cgcagcgtag cgagctgtcc ttccagtttt
     9661 tccagcagca gggcggtttt cacgataaga cctcaatgaa aatgataatt gttatgctaa
     9721 agtagccact ctgtaagctg acaacactca atatgcaacg aatcatttta atcatcattg
     9781 gctggctggc ggtagtgctg ggtacgctgg gcgtggtatt accggtatta ccgacgacgc
     9841 cgtttatcct gctggcggcc tggtgctttg cccgttcttc cccgcgcttt cacgcctggt
     9901 tgctgtaccg ctcatggttt ggcagctatc tacgtttctg gcagaaacat catgcgatgc
     9961 cacgcggcgt caaaccgcgg gcgattttgc ttattttgct cacgtttgcc atttctctgt
    10021 ggttcgtcca gatgccatgg gtgcgcatca tgttgctggt aattctcgcc tgtttgcttt
    10081 tctatatgtg gcgaattccg gtgattgatg aaaagcaaga aaagcactga agcacaacaa
    10141 tcgcagttgc aattattgcg tacagccagt acattctggc gttttcgagc acaggcgcag
    10201 gcggtcaaag gttaaacaac tgttactttt gatacgttta aaacgcgccg tgagtaccac
    10261 cgtaacaagc aggcatacac ttatgaccgc gactgcacag cagcttgagt atctcaaaaa
    10321 tagcatcaaa agcattcagg actacccaaa acccggcatt cttttccgcg atgtcaccag
    10381 cttactggaa gacccgaaag cttacgctct cagcatcgac ttgctggttg agcgttacaa
    10441 aaatgcgggc attaccaaag ttgtcggcac cgaagcgcgt ggcttcttgt ttggcgctcc
    10501 ggtagctctg ggtctgggcg ttggctttgt gccggtccgt aaaccgggca aactgccgcg
    10561 tgaaactatc agtgaaactt acgatctgga atacggcacc gatcagctgg agatccacgt
    10621 tgatgccatc aaaccgggcg acaaagttct ggtggtggac gatctgctgg caaccggcgg
    10681 cactatcgaa gcgaccgtta aactgatccg tcgtctgggt ggtgaagtgg ctgacgctgc
    10741 gttcattatc aacctgttcg atctcggcgg cgaacagcgt ctcgaaaaac agggcattac
    10801 cagctacagc cttgtcccgt tcccgggcca ttaattatcg ccagtcttgt gctgcccacg
    10861 ctacggacag cacaagatgt gcattcagcc tcgccgttct gacggggctg tgttagcatt
    10921 accccttcgt gaatccacct tccagcgttt cagagcctgc caatgagtta tcaggtctta
    10981 gcccgaaaat ggcgcccaca aacctttgct gacgtcgtcg gccaggaaca tgtgctgacc
    11041 gcactggcga acggcttgtc gttagggcgt attcatcatg cttatctttt ttccggtacc
    11101 cgtggcgtcg gaaaaacctc tatcgcccga ctgctggcga aggggctaaa ctgcgaaacc
    11161 ggcattaccg cgacgccgtg cggcgtgtgc gataactgtc gtgaaatcga gcaggggcgc
    11221 tttgtcgatc tgattgaaat cgacgccgcc tcgcgcacca aagttgaaga tacccgcgac
    11281 ctgctggata acgtccagta cgctccggcg cgtggtcgtt tcaaagttta tctgatcgac
    11341 gaagtgcata tgctgtcgcg ccacagcttt aacgcactgt taaaaaccct tgaagagccg
    11401 ccggagcacg ttaagtttct gctggcgacg accgatccac agaaattgcc ggtgacgatt
    11461 ttgtcgcgct gtttgcaatt tcatctcaag gcgctggatg tcgagcaaat tcgccatcaa
    11521 cttgagcaca tcctcaacga agaacatatc gctcacgagc cgcgggcgtt gcaattgctg
    11581 gcgcgcgccg ctgaaggcag cctgcgagat gccttaagtc tgaccgacca ggcgattgcc
    11641 agcggtgacg gccaggtttc aacccaggca gtcagtgcga tgctgggtac gcttgacgac
    11701 gatcaggcgc tgtcgctggt tgaagcgatg gtcgaggcca acggcgagcg cgtaatggcg
    11761 ctgattaatg aagccgctgc ccgtggtatc gagtgggaag cgttgctggt ggaaatgctc
    11821 ggcctgttgc atcgtattgc gatggtacaa ctttcgcctg ctgcacttgg caacgacatg
    11881 gccgccatcg agctgcggat gcgtgaactg gcgcgcacca taccgccgac ggatattcag
    11941 ctttactatc agacgctgtt gattggtcgc aaagaattac cgtatgcgcc ggaccgtcgc
    12001 atgggcgttg agatgacgct gctgcgcgcg ctggcattcc atccgcgtat gccgctgcct
    12061 gagccagaag tgccacgaca gtcctttgca cccgtcgcgc caacggcagt aatgacgcca
    12121 acccaggtgc cgccgcaacc gcaatcagcg ccgcagcagg caccgactgt accgctcccg
    12181 gaaaccacca gccaggtgct ggcggcgcgc cagcagttgc agcgcgtgca gggagcaacc
    12241 aaagcaaaaa agagtgaacc ggcagccgct acccgcgcgc ggccggtgaa taacgctgcg
    12301 ctggaaagac tggcttcggt caccgatcgc gttcaggcgc gtccggtgcc atcggcgctg
    12361 gaaaaagcgc cagccaaaaa agaagcgtat cgctggaagg cgaccactcc ggtgatgcag
    12421 caaaaagaag tggtcgccac gccgaaggcg ctgaaaaaag cgctggaaca tgaaaaaacg
    12481 ccggaactgg cggcgaagct agcggcagaa gccattgagc gcgacccgtg ggcggcacag
    12541 gtgagccaac tttcgctacc aaaactggtc gaacaggtgg cgttaaatgc ctggaaagag
    12601 gagagcgaca acgcagtatg tctgcatttg cgctcctctc agcggcattt gaacaaccgc
    12661 ggtgcacagc aaaaactggc tgaagcgttg agcatgttaa aaggttcaac ggttgaactg
    12721 actatcgttg aagatgataa tcccgcggtg cgtacgccgc tggagtggcg tcaggcgata
    12781 tacgaagaaa aacttgcgca ggcgcgcgag tccattattg cggataataa tattcagacc
    12841 ctgcgtcggt tcttcgatgc ggagctggat gaagaaagta tccgccccat ttgatcgtaa
    12901 gcacagctta cgttcgtcat ccttaacgtg attgagagag aaacctatgt ttggtaaagg
    12961 cggtctgggt aacctgatga agcaagccca gcagatgcaa gaaaaaatgc agaaaatgca
    13021 ggaagagatc gcgcagctgg aagtcaccgg cgaatctggc gcaggtctgg taaaagtgac
    13081 catcaacggt gcacacaact gccgtcgcgt agagatcgac ccgagcctgc tggaagacga
    13141 caaagagatg ctggaagacc tggtggctgc agcattcaac gacgcagcac gtcgtattga
    13201 agaaacgcag aaagaaaaaa tggcctctgt atcctccgga atgcagctgc cgcctggctt
    13261 taagatgccg ttctgatgca aaccagcccg ctgttaacac agcttatgga agcactgcgc
    13321 tgtctgccgg gcgttggccc gaagtcggcg cagcgtatgg cgttcacgct gcttcagcgc
    13381 gatcgtagcg gcgggatgcg tctggcgcag gcgctcaccc gggcgatgtc ggaaatcggc
    13441 cactgcgccg attgccgcac tttcaccgaa caggaagtct gtaacatctg ttcgaatccg
    13501 cgtcgtcagg aaaacggtca aatctgtgtg gtggagagtc cggcggacat ctacgccatt
    13561 gagcagacgg ggcagttttc aggccgttat tttgtgttga tggggcacct gtcaccgctg
    13621 gacggcatcg gtccggatga tatcggcctt gatcgtctgg aacagcgtct ggcagaggaa
    13681 aaaatcactg aagtgatcct cgccaccaat cccacggttg aaggtgaagc taccgctaac
    13741 tacattgccg agctttgcgc gcaatatgac gtggaagcca gccgaatcgc tcatggcgta
    13801 ccggttggcg gcgaactgga aatggtcgat ggcaccacgc tgtcacactc ccttgccggg
    13861 cgtcataaga ttcgttttta agcaaacgag agcaggatca cctgctctcg cttgaaatta
    13921 ttctcccttg tccccatctc tccctcatcc tgtttttaac cttaaaatgg cattattgag
    13981 gtagacctac atgaaaggac aagaaactcg tggttttcag tcagaagtga aacagcttct
    14041 gcacctgatg atccattctc tctattccaa taaagaaatc ttcctgcgtg agcttatctc
    14101 taacgcctcc gatgcggcgg acaagctgcg tttccgtgcg ctatctaacc cggacctgta
    14161 cgaaggtgat ggcgaactgc gcgttcgtgt ctctttcgat aaagacaagc gcacgctgac
    14221 catctccgat aacggcgtgg ggatgacccg cgacgaagtg attgaccatc tggggactat
    14281 cgctaaatcc ggtaccaaat cattcctcga atccctgggt tctgaccagg cgaaagacag
    14341 ccagctgatc ggtcagtttg gtgttggttt ctactctgcg tttatcgtgg ccgacaaagt
    14401 gaccgtgcgt actcgcgcgg caggcgaaaa accagaaaac ggcgtcttct gggaatcggc
    14461 tggcgaaggt gaatataccg ttgccgacat caccaaagaa gatcgtggta ctgaaatcac
    14521 cctgcatctg cgtgaaggcg aagacgagtt cctcgatgac tggcgcgtgc gttccatcat
    14581 cagcaaatac tccgaccata tcgcgctgcc ggtagagatc gaaaaacgcg aagagaaaga
    14641 cggcgaaacc gttatctcct gggagaaaat caacaaagcg caggcgctgt ggactcgtaa
    14701 caagtcggaa atcaccgatg aagagtacaa agagttctac aaacacatcg cccacgactt
    14761 taatgatccg ctgacctgga gccacaaccg tgttgaaggt aagcaggagt ataccagcct
    14821 gctgtacatc ccgtcccagg ctccgtggga tatgtggaac cgcgatcata aacacggcct
    14881 gaaactgtat gttcagcgtg tgttcatcat ggacgacgca gaacagttca tgccgaacta
    14941 tctgcgcttc gtgcgtggtc tgattgactc cagcgatctg ccgctgaacg tttcccgtga
    15001 aatcctccag gacagcacgg taacgcgtaa cctgcgcaat gcgctgacca agcgtgtgct
    15061 gcaaatgctg gaaaaactgg cgaaagacga cgcggaaaaa taccagacct tctggcaaca
    15121 gtttggcctg gtactgaaag aaggtccggc ggaagatttc gctaaccagg aagcgatcgc
    15181 caaactgctg cgttttgctt ctacccatac cgattcttct gcgcagaccg tatctctgga
    15241 agactacgtt tcccgcatga aagaagggca ggagaaaatc tactacatca ccgcagacag
    15301 ctatgcggca gcgaagagca gcccgcacct ggaactgctg cgtaagaaag gcatcgaagt
    15361 tctgctgctt tccgaccgca tcgatgagtg gatgatgaac tatctgactg agttcgacgg
    15421 taaaccgttc cagtcggtgt ctaaagttga cgagtcgctt gaaaaactgg ctgacgaagt
    15481 tgatgagagc gcgaaagaag cggagaaagc actgactccg ttcatcgacc gtgtgaaagc
    15541 cctgctcggc gagcgcgtga aagatgtccg tctgactcac cgtctgaccg atacgccagc
    15601 gatcgtttcg accgacgcgg acgaaatgag cactcagatg gcgaaactgt tcgctgcggc
    15661 gggccagaaa gtgccagaag tgaaatacat cttcgaactg aacccggatc acgtactggt
    15721 gaaacgtgcg gcagatactg aagatgaagc gaagttcagc gagtgggtag aactgctgct
    15781 ggatcaggcg ctgctggcag aacgcggcac gctggaagat ccgaacctgt ttattcgtcg
    15841 tatgaaccag ctgctggttt cctgatgtca tgccggatga cctttgtgtc atccggcatt
    15901 tttcttttca tcatctgcac tttccgcaaa ttatctcgcc attaaccgtt tcagcctcag
    15961 gtgcctttct tgaggcaatc gcctgttggt ggtatcgttt atcgcttttt caaaaaattc
    16021 gacacatttt aaggggattt tcgcaatgcg tatcattctg cttggcgctc cgggcgcggg
    16081 gaaagggact caggctcagt tcatcatgga gaaatatggt attccgcaaa tctccactgg
    16141 cgatatgctg cgtgctgcgg tcaaatctgg ctccgagctg ggtaaacaag caaaagacat
    16201 tatggatgct ggcaaactgg tcaccgacga actggtgatc gcgctggtta aagagcgcat
    16261 tgctcaggaa gactgccgta atggtttcct gttggacggc ttcccgcgta ccattccgca
    16321 ggcagacgcg atgaaagaag cgggcatcaa tgttgattac gttctggaat tcgacgtacc
    16381 ggacgaactg attgttgatc gtatcgtagg ccgccgcgtt catgcgccgt ctggtcgtgt
    16441 ttatcacgtt aaattcaatc cgccgaaagt agaaggcaaa gacgacgtta ccggtgaaga
    16501 actgactacc cgtaaagacg atcaggaaga aaccgtgcgt aaacgtctgg ttgaatacca
    16561 tcagatgact gcaccgctga tcggctacta ctccaaagaa gcggaagcgg gtaacaccaa
    16621 atacgcgaaa gttgacggca ccaagccggt tgctgaagtt cgcgctgatc tggaaaaaat
    16681 cctcggctaa ttcgaaagcg cgcacggaca gtcccctcgc cccctcgggg agagggttag
    16741 ggtgagggga acaggcccgc acaagcaaac ttatcagcaa tctcaggccg gatattcatt
    16801 cggcctttta caaaaacctc aatctgcgct attccttttt ctgatttgac ctctcacagc
    16861 aattagttct tcttcctcac ttttccgcta caattatcaa caagttgaat cgataagagg
    16921 cggtaatgcg tcagactaaa accggtatcc tgctggcaaa cctgggtacg cccgatgccc
    16981 ccacacctga agcggtaaaa cgctatctga aacaattttt aagcgacaga cgcgtggttg
    17041 atacctcacg gttgttatgg tggccgttgc tgcgcggcgt gattttgccg ctgcgctcgc
    17101 cgcgtgtggc gaagctgtac gcctcggtct ggatggaaga cggctcgccg ctgatggttt
    17161 acagccgcca gcaacagcag gcgttggcac aacgtttacc ggatacgccc gtagcgctgg
    17221 gcatgagcta cggctcgcca tcactggaaa gcgccgtcga tgaactcctg gcagagcatg
    17281 tagatcatat tgtggtgctg ccgctttatc cgcaattctc ctgttctacg gtcggtgcgg
    17341 tatgggatga actggcacgc attctggcgc gcaaacgtag cattccgggg atatcgttta
    17401 ttcgtgatta cgccgataac cacgattaca ttaatgcact ggcgaacagc gtacgcgctt
    17461 cttttgccaa acatggcgaa ccggatctgc tgctgctctc ttatcatggc attccccagc
    17521 gttatgcaga tgaaggcgat gattacccgc aacgttgccg cacaacgact cgcgaactgg
    17581 cttccgcact ggggatggca ccggaaaaag tgatgatgac ctttcagtcg cgctttggcc
    17641 gggaaccctg gctgatgcct tataccgacg aaacgctgaa aatgctcgga gaaaaaggcg
    17701 taggtcatat acaggtgatg tgcccgggct ttgctgcgga ttgtctggag acgctggaag
    17761 agattgccga gcaaaaccgt gaggtcttcc tcggtgccgg cgggaaaaaa tatgaatata
    17821 ttccagcgct taatgccacg ccggaacata tcgaaatgat ggctaatctt gttgccgcgt
    17881 atcgctaaag ctgagcggta aagaactgag cgccgtcgcg aagagcctcg tcggcggttt
    17941 tcatcattcg tgaataatgc aaaaaggcat gcagcgtacc tgggtagagt ttgaactcac
    18001 agggctgctg atgcgccgct aacgtctggt aaagcagacg gctgtcatcc agcagcggat
    18061 cgaactccgc cccggcaata aaacagggcg gaacttcgcg agtgagatca ttattaaaca
    18121 gacagtaata cggtgactcg cggtccgcgt cgttgcttaa atacgcctct tcatacatct
    18181 gcaaatcctg ttgcgttaag ccatcccaga caccgcccaa cagacgacga gtcacggaat
    18241 cccgtaatcc gtaaagccca taccacagca aaacacccgc aattttaccg caatcgatct
    18301 gtttatcacg caaccacaac gcactggcga gcgccagcat ggcacctgcg gaatcaccgg
    18361 caaagccaat gcgggacata ttgatttgat aatcctccac ctgctggtgg aaataacaac
    18421 aagcagccac aatttcctct atcgcttgcg gaaaacgcgc ttcaggtgaa agggtgtaat
    18481 caataccaat caccgtacat tggctgtagc ttgccagcag gcgcatgatg cgatcgtggg
    18541 tatcgagatt gccgagaata aaaccgcctc catgcaaata aaatagcgtc gccgggctat
    18601 ctggctgcgg acaaaagaga cgtgtttcca cctgcccata ttttgttgga accatgtaag
    18661 ctctggttgc catttctgga gcgcccgcat tccagaatcg gcgctcaagc gtgtaatact
    18721 gtcgttgctc agcaatcgtt cccgttgcgg gccagggcgg taaatccggc tgaagagtat
    18781 tcacaacggt cttcattcca gcagaaataa ggtccagaac aggtaatttg ttttccggct
    18841 tcatacaaaa actcctttca aattacgtca ttgtaaggaa ccactgccat gaaaatgcga
    18901 tcccgcctgc tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga ttgtgttaac
    18961 attcgccgct cagttaacca cccgtaaaaa caaccatgaa atttcccggt aaacgtaaat
    19021 ccaaacatta cttccctgta aatgcacgcg atccgctgct tcagcaattc cagccagaaa
    19081 acgaaaccag cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat attgaagcga
    19141 aagtggatga tgaattcatt gagcgttatg gattaagcgc cgggcattca ctggtgattg
    19201 aggatgacgt agccgaagcg ctttatcagg agctaaaaca gaaaaacctg attacccatc
    19261 agtttgcggg tggcaccatt ggtaacacca tgcacaacta ctcggtgctc gcggacgacc
    19321 gttcggtgct gctgggcgtc atgtgcagca atattgaaat tggcagttat gcctatcgtt
    19381 acctgtgtaa cacttccagc cgtaccgatc ttaactatct acaaggcgtg gatggcccga
    19441 ttggtcgttg ctttacgctg attggcgagt ccggggaacg tacctttgct atcagtccag
    19501 gccacatgaa ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc ggagcctcgg
    19561 cactggttct cacctcatat ctggtgcgtt gcaagccggg tgaacccatg ccggaagcaa
    19621 ccatgaaagc cattgagtac gcgaagaaat ataacgtacc ggtggtgctg acgctgggca
    19681 ccaagtttgt cattgccgag aatccgcagt ggtggcagca attcctcaaa gaccacgttt
    19741 ctatccttgc gatgaacgaa gatgaagccg aagcgttgac cggagaaagc gatccgttgt
    19801 tggcatctga caaggcgctg gactgggtag atctggtgct gtgcaccgcc gggccaatcg
    19861 gcttgtatat ggcgggcttt accgaagacg aagcgaaacg taaaacccag catccgctgc
    19921 tgccgggcgc tatagcggaa ttcaaccagt atgagtttag ccgcgccatg cgccacaagg
    19981 attgccagaa tccgctgcgt gtatattcgc acattgcgcc gtacatgggc gggccggaaa
    20041 aaatcatgaa cactaatgga gcgggggatg gcgcattggc agcgttgctg catgacatta
    20101 ccgccaacag ctaccatcgt agcaacgtac caaactccag caaacataaa ttcacctggt
    20161 taacttattc atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc tatcaggtac
    20221 tgaaccagca ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac agcctggaag
    20281 agtcttactg ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc gttcacgccg
    20341 catctggcat ttggctctcg atgcctgatg cgacgctggc gcgtcttatc atgcctacat
    20401 atttttcata ttttacatcc ggcaaccacc gtttaccccg tcaccacctc acccgccggt
    20461 ggcgtttcca gcagttccag catggtacgg gcgatttcac gctcgcccat cactacctga
    20521 ttcgcaccac gttcggtgat atacgccact tcatcgtcat aatgggcgcg ggcaataatc
    20581 tcaatatccg gatttttcgc gcgggcagat gccacaatct cacccgcttc ataaccgttg
    20641 ggaatcgtca ggatcagcca ttttgcacat tccagatgcg ccagttgcat aatttcttcg
    20701 ttcgccgcat tgcccaatac tgcgcggacc ccgcgctctc gcagctcatc aacacgggtt
    20761 cgtgacgtct caatcaccac cagcggaata tcagaggcga gcaatttctc ccccagcagg
    20821 ctgcctacac gaccgtaacc caccagtagc gcatggttgc aaatatccac tgggatctgc
    20881 ttctcttctt cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt cttcgccaga
    20941 tatttctcca gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat cgcccctgcc
    21001 agtaccaggt tttgtccggc ctgcggcagt aaattcaatg ccattcccag tcccgccagg
    21061 ataaacgcga actcaccaat ctgcgccagg ctggcggcga tggttaatgc cgtacgttgg
    21121 gagtgaccaa acagtcgcac caggaaaaat gcggctaacg acttaccaaa cagaataatc
    21181 gccagcgtcg ccagcactgc cagcggttgc tgaatcagaa ttaacggatc aaacaacatc
    21241 ccgacggaga caaaaaacag caccgcaaac gcgtcgcgca atggcagcgt atcgtgggcg
    21301 gcacggtgac tcagttcaga ctcgttcagt accatcccgg caaagaacgc accgagtgca
    21361 aaggagacat caaacagctc taccgcacca aaggcaaccc ctaacgccag cgccagcacc
    21421 gacagggtaa acagctcgcg agaaccggtt gccgcgctgc gtgccataat ccacggcacc
    21481 agacggcgac ctaccagcat cataatggcg ataaatgcga tcactttgcc gatggtgatc
    21541 cccatatcga ctgcaagagt ggcaaagccc acatcgccct gttccatcat tcctgccact
    21601 gcgggcagca acaccagcgt cagaaccatt accaggtctt ccacaatcaa ccaaccgatg
    21661 gcgatttgcc cacgctgact gtcaattaat tgccgttctt caagtgcgcg cagtaacacc
    21721 acggtactgg cggtggaaag acataaaccg aacacgatac cggtcattaa cgaccagccc
    21781 agcacggcag agagcgccat acccagcagc gtcgccacgg ctatctgggc gatcgcaccg
    21841 ggaatggcga tggcctttac cgccatcaaa tccttcagcg aaaagtgcaa accgacgcca
    21901 aacatcaaca gaatgacgcc cagttcagcc agttccgggg caagcttggt atcggcaaca
    21961 aagcccggag tgaatggtcc tgccagcaca cccgctaaca gatatcccac cagaggagaa
    22021 atacgtagtt tattggccag catgccgagg ataaaggcga gcacaaggcc gccaacaatg
    22081 gtggtgataa gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg ttattgtcca
    22141 tttttggccg ggaaaaccaa aattacaggt aatagtttat gacaatttca ttgatgatgt
    22201 tcatgaataa ttattgaatt ttgcagaaaa atggaattag ctgcaaaaaa gcacggatcg
    22261 gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac gatgcagcca
    22321 aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc aggaatatgg
    22381 tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg atgctggtgt
    22441 gatcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa gcaaaaccga
    22501 aaaagagtcc agaaaccata ccgatacgtc ctggaagcag ctcctgagcg tagaccagaa
    22561 tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc cccgtccagt
    22621 gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata gagccccaaa
    22681 tcacatattt ccgcccaatt ttatccccta caggcccgcc gatcaccgta cctgccgcaa
    22741 cggcaaacag gaaggcaaac agatgaagct gagcattctg gatagataat ccgaattttt
    22801 gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat ttcgagaaaa
    22861 tgaggattaa cagaatgctg accgccagta caactttatt gcgcggcagt ggattgataa
    22921 tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa cggctgattt
    22981 gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg ttgcctttgc
    23041 cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca aagttgccgc
    23101 cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata cgggccacgc
    23161 gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc gccagcagaa
    23221 ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc gcttaaggta aagcacatgc
    23281 caattggcaa cgaccatggc atcggatatt tatcggtcca gtagccgacc actggttgca
    23341 gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata aatgtcagag
    23401 aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc atgtcgttca
    23461 gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc ttggtcgttg
    23521 acgcagccgc gcccgccaca ggctggggtt gttcactcat tgccatagga aagtcacttt
    23581 ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact aacatacctg
    23641 tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct ggatgcagat
    23701 ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt tcaacacgtt
    23761 gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg ttagcgctgt
    23821 taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat aaaacctaca
    23881 aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat gaatatggcg
    23941 aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag gttgcggctg
    24001 aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg cccgagtctg
    24061 acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat gacgcgatgg
    24121 cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag gaaaagtggg
    24181 ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag cgcctgttta
    24241 aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg ctgacaaccg
    24301 atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt cgtaagcccg
    24361 ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca gacattatta
    24421 tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac gcaccgggcg
    24481 atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc ggtggtcact
    24541 cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac gtgccgggta
    24601 cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat gagtggggca
    24661 aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg gttaactacc
    24721 agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa agcgagcgcg
    24781 tgctgtacac ccctgaaatc gctgaaaacc agcaaatgat ctcgctgtta tcgccgttcc
    24841 agaacaaagg caaagcgcag ttggaagtga aaataggcga aaccaatggt cgtctggaag
    24901 gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttaatt ctggcagcac
    24961 aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt cgtgattcta
    25021 tcgaagcagg cgatatcagc tataaaaacg tactgaaagt gcagccattc ggcaatgtgg
    25081 tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc gtcgcgcaga
    25141 tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg gcgaaagacg
    25201 gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccagcgaaa acttaccgta
    25261 tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt gataacaaac
    25321 cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat atccagaaaa
    25381 gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg cagtaatccg
    25441 aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat atcagcaaat
    25501 ttggcatcga ggatctttgc cagagcgcct gccgccagtt cgatatccag tccgcgcttg
    25561 ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat cgttggcaga
    25621 cgttttttct gccccagtgg gctaatccct ccaaccaggt atcccgtcga acgctgcgcg
    25681 accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac ttttttaaga
    25741 tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc accgttcact
    25801 gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt gacgacttca
    25861 tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa cgaaatcttg
    25921 tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta aacaaaaaaa
    25981 cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc taagagtatt
    26041 ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt gacggcataa
    26101 taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca tttttttagc
    26161 gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc gcgaccacat
    26221 atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc tgatgcatca
    26281 gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta ttttgcggag
    26341 gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta tgccagtggg
    26401 tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac atggcaatct
    26461 ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgtttcgcc gcctgcaata
    26521 gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc gtcgcctgaa
    26581 gaaccatcgc gatttgccac agcggttggg tagaaaagag cgagggagaa atgcccatct
    26641 cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct tccagcgcct
    26701 cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc tcgacgatca
    26761 gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc atatcgtggc
    26821 tacccatatg aatactgcca atcagatgaa aatggcgatt gccgggaagg gtgatatcga
    26881 tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt acccggtaca
    26941 acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga gggatggcgc
    27001 aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct tgtatcgcta
    27061 ttcacaggga agcaacagtt aaaaactatg aaacaggcaa caagaaaacc gacgacaccc
    27121 ggagatattc ttctctatga atatctggaa ccgctagatt tgaaaatcaa tgagttagca
    27181 gagttgctgc atgttcatcg taatagcgtc agtgcactga tcaataacaa tcgtaaactc
    27241 accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga tttttggcta
    27301 aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac ccaggaagaa
    27361 ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg tgcaaaaaag
    27421 gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt cacgccgcat
    27481 ccggcatccg agcaccattg cctgatgcga cgcttacccg tcttatcagg cctacaaacc
    27541 tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcatcc gagcaccatt
    27601 gcctgatgcg acgcttaccc gtcttatcag gcctacaaac ttgtgcctga accgtaggcc
    27661 ggataaggcg tttacgccgc atccggcagt catgcgtcga tgccaaatgc gccgccctaa
    27721 agcagcgcat ccgcaatgac gtacttattc cttcggttta aaccgcagca accggttggc
    27781 gttactcact acggtaatcg acgagagcgc cattgccgct ccggcaacca ccgggttaag
    27841 cagtgttcca gtaaacggcc acaaaatacc ggcggcgacc ggaataccga tactgttgta
    27901 gataaacgcg ccgagcaggt tctgcttcat gttgcgcagc gttgcgcggg aaatagcgag
    27961 cgcatccgca acgcccatca ggctatggcg catcagggta atcgccgcgg tttcaatggc
    28021 aacatcactg ccgccgccca ttgcaatgcc gacatccgcc tgggccagcg ctggcgcatc
    28081 gttaatgccg tcgcccacca ttgccacctg acgtccttca ctttgcaggc gtttaatcgc
    28141 ttcggcttta ccgtccggca gcaccccggc gatcacttca tcaatccctg cttctttggc
    28201 gatcgcattg gcggtggttg ggttatcccc ggtcaacatc accagacgat atcccgcttt
    28261 atgcaggcgt tgcagcgccg ccacgctatc actacgcaat ggatcgcgta ctgccagcag
    28321 ggctaccgct ttcccgtcaa ccgccagcag cacaggcgtt gccccttgcg atgcctgagc
    28381 agtaatctcc gcttcgatag ctttggtacc aacctgttgc tcatttaaca gcgcctgatt
    28441 gcccagcaat aacgcatgac cttcagcttc accgctcacg cccagcccgc gcaatgtgcg
    28501 gaaaccgttg acctgcggca gctgcatatc acctgcttta tcgaggatcg cccgtgccag
    28561 cggatggctg gaaccttgct ccagtgccgc cgccagacgc aatgcctgcg cttcatcaac
    28621 atcagcaaac gttttcactg cgacaacctg cggcttccct tcggtcagcg tcccggtttt
    28681 atcgaacacg acggtatcga gtgtactggc gcgttgcagc gcgtcggcgt cccgcaccag
    28741 cacgccaaac tcagccgccc gcccgacgcc ggaaataatc gacatcggcg tcgccagccc
    28801 cagcgcacac ggacaggcga taatcagtac cgtggtggca atcaccaggg tataaacaat
    28861 ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg accagcgcaa tcaccaccac
    28921 cacaggtaca aatacggctg agattttatc cgccagctga ccgatttctg gcttgctgct
    28981 ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg gtatggctgc caaccgcact
    29041 ggcacgaaac agcacactgc cgtcctgtac cactgtcccg gcatggacgc tttcgccttc
    29101 gcctttttgc tgtgggattg gttcgcccgt cagcatcgct tcatccagcc atgcttcgcc
    29161 ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc gtcaggcgca gtaacatccc
    29221 tggctgaact tccgccagcg gcacgctttt ttcaccctct gcggtgacca ctcgcgccgt
    29281 cggtggggtt aaatcgagta atttctccag cgccttagaa gaacgctgac gtgcgcgcgc
    29341 ttccagcata tggccgagat taatcagacc gataatcatc gcactggctt catagtaaag
    29401 atgccgcgct tccatcggga accactgcgg ccacaggttg acgctcatcg aatagagcca
    29461 cgccacaccc gttcccagcg ccaccagcgt atccatcgtc gctgcaccgt tcagcaggct
    29521 tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc atcactgcca gggttatcag
    29581 gccgataact aaccacaagc tgcggttgtc agcggtgacc atcatgttat cgccgatcat
    29641 cccccagacc attaccggga tacccacagc cagtgcaaca attgcctgcc agcggaagcg
    29701 cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg cgtttagcgt catcttcaat
    29761 cgcttccgca ccgtagcccg ctttttccac cgcctgcact aaatcttgtg gggaggcact
    29821 gcccatcacc agcgcagtac gctccgccag gtttacccgt gcctgagtga cgcccggtac
    29881 gctttgcagc gcattttgta cgcgggtgac acagctggcg cagctcatgc cgctcagcag
    29941 caactgctgg ctgtcatcgt catcggcggt cgctgccgga agcgcctcag aaaccgctgt
    30001 cagtgcttcc gacgggattg atgactccgc cagcggttta gcctttgggt ggcttacaga
    30061 tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt tctgcactgg cagtcccggt
    30121 aacgtgcgct tcagtgatag acacatccgc ctgctcaaca tccgggcgct gttcaagact
    30181 ttctttcacg cgtttaacgc agtgaccgca ggacaggccg tccagggtca ggtcgatagt
    30241 ttgtgacata aaacactcct ttaagacagt tttgactggc tgtgataaag gttaaacctt
    30301 ccagcaaggg gaaggtcaag aaattaataa accaggcgag taaaagtccg taaagattaa
    30361 aaaattggct caatttgcat caggattaga catttatctc tttgttttcc tgtagttaag
    30421 ttgcgggtgc taagttaaat caggatgcct gaaaatcggc accggggtga ggaattacct
    30481 cccgcatcta taaaaaggag ttaacaaaag atgttagatg caaacaaatt acagcaggca
    30541 gtggatcagg cttacaccca atttcactca cttaacggcg gacaaaatgc cgactacatt
    30601 ccctttctgg cgaatgtacc tggtcaactg gcagcagtgg ctattgtgac ctgcgatggc
    30661 aacatctatc gcgcgggtga tagtgattac cgctttgcgc tggaatccat ctcgaaagtc
    30721 tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg cggtacagga caaaattggc
    30781 gctgacccga ccggattgcc ctttaactca gttatcgcct tagagttgca tggcggcaaa
    30841 ccactgtcgc cactggtaaa tgctggcgct attgccacca ccagcctgat taacgctgaa
    30901 aatattgaac aacgctggca gcgaatttta catatccaac agcaactggc tggtgagcag
    30961 gtagcgctct ctgatgaagt caaccagtcg gaacaaacaa ccaacttcca taaccgggcc
    31021 attgcctggc tgctgtactc cgccggatat ctctattgtg atgcaatgga agcctgtgac
    31081 gtgtacaccc gtcagtgctc cacgctcatc aatactgttg aactggcaac gcttggcgcg
    31141 acgctggcgg cgggtggtgt gaatccgctg acgcataaac gcgttcttca ggccgacaac
    31201 gtgccgtaca ttctggccga aatgatgatg gaagggctgt atggtcgctc cggtgactgg
    31261 gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg gcggtattct ggcggtcgtc
    31321 cctggcgtga tgggaattgc cgcgttctca ccaccgctgg acgaagaagg caacagtgtt
    31381 cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg gctataacgt gtttaagggc
    31441 tgatcatgat gaacacggaa ggtaataatg gtaacaaacc tctcggtcta tggaacgtcg
    31501 tttccatcgg cattggggca atggtggggg cggggatctt cgcgctgctg gggcaggctg
    31561 cattgctaat ggaagcctcg acctgggtcg cctttgcttt tggcggtatt gtggcgatgt
    31621 tttccggtta tgcctatgcg cgtctggggg cgagctatcc cagcaatggc ggtattatcg
    31681 acttttttcg tcgcggatta ggcaacggcg tcttttcgct ggcgctctcg ttactgtacc
    31741 tgttgacgct ggcggtgagc atcgccatgg tcgcccgtgc ttttggcgct tatgccgtgc
    31801 agtttttgca tgaaggcagc caggaggagc accttatttt gctctacgcg ttggggatca
    31861 ttgcggtgat gacgcttttc aactccttaa gcaaccatgc ggtagggcgg ctggaagtga
    31921 tcctcgtcgg cattaaaatg atgatcctgt tattgctgat tattgccggt gtctggtcgc
    31981 tgcaaccggc gcatatttcc gtctctgcgc cccccagctc cggtgcgttc ttctcctgta
    32041 tcgggataac cttccttgcc tatgcgggct ttggcatgat ggcgaacgcg gcggataaag
    32101 tgaaagatcc gcaggtcatt atgccgcggg cgtttctggt ggcgattggc gttaccacgt
    32161 tgctttatat ctcgctggca ctggttttgc ttagcgatgt atcggcatta gagttagaaa
    32221 aatatgccga taccgccgta gcgcaggctg cttctccgct gctcggacat gtgggttatg
    32281 tgatcgtcgt catcggcgct ttactggcga cggcttcagc cattaacgcg aacctgttcg
    32341 ccgtgtttaa catcatggac aacatgggca gcgaacgcga actgccgaag ctaatgaata
    32401 aatccctgtg gcggcagagt acctggggca acatcattgt cgtggtgttg attatgctga
    32461 tgacggcggc actgaattta ggctcactcg ccagcgttgc cagcgccacc tttttgattt
    32521 gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac gcctcgttgc
    32581 cgattcttat cgttggtacg ttggtgatgt tgttggtgat cgttggcttt atctacagtc
    32641 tgtggtccca gggtagccgt gcgttgatat ggattattgg ctcactctta ctcagcctta
    32701 ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca tctctctgtg cgcagtactt
    32761 cctgtattat tgtggtggcg gtcgatattc gcactggcaa aaaaacgtgc ttgaatatct
    32821 gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat cagcgatgta
    32881 gcaaaaatta ccggcctgac tagcaaagcc attcgcttct atgaagagaa ggggctggtg
    32941 acgccgccga tgcgcagcga aaacggttat cgcacctaca cgcagcagca tctcaacgaa
    33001 ctgaccttac tgcgccaggc acggcaggtg ggctttaacc tggaagagag cggcgagctg
    33061 gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg tcaaacggcg cacgctggag
    33121 aaggtggcgg agatcgaacg acacattgag gagctgcaat ccatgcgcga ccagctgctg
    33181 gcactggcga atgcctgccc tggcgatgac agcgccgact gcccgattat cgaaaatctc
    33241 tccggctgct gtcatcatcg ggcagggtga ttaagacgag acggctcgga tatgtagggt
    33301 tatcccttct atcgcaatga cttcaacatg cgtacctgca ccgagatcct cgctggcgct
    33361 gacaggccat gaactgtcac cgacgcgcat atgaccgcgc ccgttgacca gcggagattc
    33421 cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg tttaaatgac tgtcgctgtg
    33481 cttttgttcg cgcacccgcc gcgacaacca tttccaccac agccaggcgg cgagcagcgt
    33541 caggacggca aacatcaccc cttgccactc ccaacccagc ggcaccagcc agaccaccag
    33601 gccggtaatc accgctgcca cgccgctcca caacaaataa ccatttccgc ccagcatctc
    33661 ggctgccagc agcaaaccgc cgagactgag ccagaaaata tgtggatgaa cgaccattaa
    33721 ctccatcatg gctgagtccg cttgttggcg ctgtctttca ccagctcggc aatcccggca
    33781 atcgacccca tcaggctgct ggcctctaat ggcatcatca ctactttgct gttgctggac
    33841 gaaccgatct gctgtaacgc ttcggtgtat ttctgcgcta cgaagtagtt caccgcctga
    33901 atatcaccgg aggcgatagc ttcagacacc attttggtgg cgcgggcttc tgcttcggcg
    33961 gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac gttcgccttc cgctttcagg
    34021 atttgcgact gtttttcacc ttcggctttg aggatttccg cctgacggat cccttccgct
    34081 tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct gcgcgttcat tgaagagata
    34141 agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg tgactttaat cccccacggg
    34201 ttggtggctt catcgacaat acgcagcagg cgtgagttga tgctgtcgcg ctgagagagc
    34261 atttcgtcaa gttccattga acccagcacg gtacggatgt tagtcatggt caggttgatg
    34321 atcgccagct ccagattgct gacttcataa gccgcgcgcg gcgcgtcaat cacctgaata
    34381 aagcacacgg cgtcgatggt aacgttggcg ttatctttcg agataacttc ctgggaaggg
    34441 atatcgagca cttgctccat catattgatc ttgcgaccaa tgcgatccat aaacggcacc
    34501 accagactga gccccggctg taacgttttg gtatagcgac caaagcgttc tactgtccac
    34561 tgatagccct gcggtacgat tttgacaccc gcgccgacaa tgaccagcgc gacaaaaatg
    34621 agaatcggga taaagataag catcggaaaa acctcctgtt gtaccgtcca taatcagcaa
    34681 aattgctgct tgattaaaca aattatacct gattactgaa agagagttcc cccttattcc
    34741 tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg gaacagcgat gcaggaaaat
    34801 agtcctttgc ttcagctaca aaacgtagga tatctggcgg gtgatgcgaa gattcttaat
    34861 aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa ttaccggtcc ttctggttgt
    34921 ggcaaaagta cgctgctaaa aatagttgct tcattgatca gcccaaccag cggaacgtta
    34981 ctgtttgaag gtgaggatgt cagcacacta aagccagaaa tctaccgcca acaagtctct
    35041 tactgcgccc agacaccgac gctgtttggc gatacggtat acgataatct gatctttccc
    35101 tggcagatcc gtaaccggca gcctgaccca gccatttttc tcgattttct cgaacgcttc
    35161 gccttgccgg acagcatttt gacgaagaat atcgccgagc tatctggtgg tgaaaaacaa
    35221 cgcatctcat tgattcgtaa cctgcaattt atgccgaagg ttttattgct ggatgaaata
    35281 accagtgcgc tggatgaaag taataaacat aacgtcaatg agatgatcca tcgttatgtg
    35341 cgcgagcaaa atattgccgt gctgtgggtg acacacgata aagacgaaat taatcatgcg
    35401 gataaagtga ttacactgca accgcatgcc ggagaaatgc aggaagcacg ctatgaactc
    35461 gcataatatt actaacgaat cattagcact ggcattaatg ctggtggtgg tggcaatctt
    35521 aattagccat aaagaaaaac tggcgctgga gaaagatatt ctctggagcg tcgggcgagc
    35581 gataattcag ctgattattg tcggctatgt gctgaagtat attttcagcg tggatgatgc
    35641 cagcctgaca ttattgatgg tgttatttat ctgctttaat gcggcgtgga acgcgcaaaa
    35701 acgcagtaaa tatattgcta aagcttttat ctcatcgttt attgccatta cggtcggggc
    35761 gggaattacc ctggcggtgc tgattctctc agggtcgatt gaatttatcc cgatgcaggt
    35821 gatccctatc gccgggatga ttgccggtaa cgccatggta gcggtggggt tgtgttacaa
    35881 caatttaggg caacgggtca ttagcgaaca gcaacagatt caggagaaac tgagtcttgg
    35941 tgcgacgccg aagcaggctt cagcgatatt gattcgcgac agtattcgcg cggctttaat
    36001 tccgacggtc gattcagcaa aaacggttgg cttagtgagt ttaccaggaa tgatgtccgg
    36061 gctgatattt gccgggattg atccggtgaa ggcgattaaa tatcagatta tggtgacctt
    36121 tatgctgctc tcaaccgcca gcttgtcgac cattattgcc tgctatttaa cctatcgtaa
    36181 gttttataat tcgcgccacc agttggtggt gacgcaattg aagaagaaat gatgcgatgc
    36241 cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc cgatcaacgg aatatcaata
    36301 caacaatgca tacagctggc ggcgatactt cgacgccagt gcatcacccg tacccagcgc
    36361 agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct gcggcggtga gatctttacg
    36421 cagatgcccg aacagcaact ccagcgcctc ttcattgcgc ccaacctgat gcagttgcag
    36481 cgccagttgc gtcgccagtg cggcatcttc tggattctcc gccacctgct gttgcaactg
    36541 ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg atttgcgcca ccagcccctg
    36601 gtagcgggtg tcctgatcct gcaacggaat ggttttcagc accgcttccg catcttcaga
    36661 acggttcagc gcaatcagcg tttctgccag cagcaggccg atctccccgt tctgattcga
    36721 caactgccag gcgtctttca gcaatggcag ggcatcggtg taattgcttt cctgcatcag
    36781 ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc agcactttat ccagcagggc
    36841 gcggatcgcc tcttccggtt gcggcccctg gaagccatct accggttgcc cgttctggaa
    36901 cagatacacg gtcggaatcg cgcgcagacc aaactgggcg gcaatcatct gctccgcgtc
    36961 gcagtccagc ttcgccagaa taaattgccc gttgtactgc gccgcgaggc tttccaggat
    37021 cggggttaac tgcaaacagt gctggctacg ttcagaccaa aaatagaaca gcaccggagt
    37081 ggtcatcgac tgttcaagaa cctgttgcag gttagattcg ttaatgttga caatattttc
    37141 tacggacatg gagtcgctct ctgttgtcga tattttcttt gacatggggg cttaagcgcg
    37201 cgcttcaact cacccctgca atattttgtc catcacgcgc cccggcagca ggcgcttaag
    37261 caccattact gcccaggtca ccagcgttac cggatagcgc atcttcggct tctcgctaat
    37321 aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc aacgtaaagc gggcggcgat
    37381 gccgggattt tcgactggtt tatcactttg cgtctggttg acgttgtcgg tgaagcgagt
    37441 acgaatggga ccgggttcga tcaggctgac tttaattccg ctgtggcgta gctccatgcg
    37501 cagcgcgtct gaccacgcct ccagcgcata tttactggcc gcgtaagcgc cacgacccgg
    37561 cgtggagatt aatcccatca ccgatgatgt catcacaata cgcccttcac cgtgcggtaa
    37621 catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg aaaaagttgg cggaaaactg
    37681 ctgttccatc tgcgcacggc tgatggtgga aagggggcca tacatgccga atccggcatt
    37741 gttaaagatc ccatacagac aattatcggt cagggcgatc acttcgtcgg ctgcgcgatc
    37801 aacactttct ggcgaatcca gatcgatcaa cacgccggta aatcccatgc tgttcatacg
    37861 ctcaacatca tccggtttcc ggcaacctgc cagcacatga aaaccctggc gttttaattc
    37921 gagcgcgctt tccaggccaa ttccactgga acatccggta attaagaccg atttttgcat
    37981 aactttacct gtcaggatct ccgttgcttt atgagtcatg atttactaaa ggctgcaact
    38041 gcttcgccat ccagtcggca ataaacggct gggcgtcgcg gttgggatga ataccgtcat
    38101 cctgcatcca ttgtggcttg aggtagacct cttccataaa aaagggcagc agcggaacat
    38161 caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc ttcattataa cggcgaccat
    38221 agtttgcagg cagacgtatt tgcattaaca atggttcagc gttggcggct ttgacatcct
    38281 gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg aaaaccacgc aaaccgtcat
    38341 tgccgcccag ttcaaccagc acccaacgcg gctgatgctg tttcagcaga gccggaaggc
    38401 gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc attaactacc gacgttttac
    38461 tctgccactt atcattcaac aaggcaggcc aggccgcgct ggcaggcatt cgatacccgg
    38521 cgctcaggct atcacccaga atcaataacg tgtccgctgc ggcggcacgg aaggttaaca
    38581 ggaccaggaa caggaagggc aaatgccagc ggaaaacatt gttgaagttc atcatcttaa
    38641 gaagtccgtc ggtcaggggg agcatgaact ctccatcctc accggagttg agctggttgt
    38701 caaacgtggc gagaccatcg cactggtggg cgagtcggga tcgggtaagt caaccttgct
    38761 ggcgatcctc gccgggcttg atgacggcag cagtggcgaa gtgagtctgg tgggacaacc
    38821 gctacataat atggacgaag aagcgcgggc aaagttgcgc gcgaagcacg tcggctttgt
    38881 ttttcagtca tttatgttaa ttcctaccct taacgcgctg gaaaacgtcg agcttccggc
    38941 tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg gcgaaagcgt tgctcgaaca
    39001 gttagggctg ggtaaacgtc tggatcatct tccggcacag ctttccggcg gtgaacagca
    39061 acgagtggcg ctggcacgag cctttaatgg tcgacctgat gtgctgtttg ccgacgaacc
    39121 caccggcaac cttgaccgcc agacgggcga taaaattgcc gacctgctgt tttccctcaa
    39181 ccgtgaacat ggcaccacgt tgattatggt gacccacgac ctgcaactgg cggcacgctg
    39241 cgaccgctgc ttacggctgg tgaacgggca gttgcaggag gaagcatgat tgcacgttgg
    39301 ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct ggctggcgct aagcctggcg
    39361 gtggcctgcg tgctggcgct gggcaatatc agcgatcgca tggagaaggg cttaagccag
    39421 caaagccgtg agtttatggc gggcgatcgg gcgttgcgca gttcacgcga agtgccgcaa
    39481 gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg gcaagcagct gactttcgcc
    39541 acaatgacct ttgcaggcga cacaccgcag ctggcgaacg tcaaagcggt ggatgatatc
    39601 tacccgatgt atggcgatct gcaaactaat ccccctggcc tgaaaccgca ggcgggcagc
    39661 gtattgctgg ccccacgcct gatggcactg cttaacctga aaacgggcga caccattgac
    39721 gtgggcgatg ccaccttgcg gattgccgga gaagtgattc aggaaccgga ttccggtttt
    39781 aaccccttcc agatggctcc gcgtctgatg atgaatctgg cggatgtcga taaaaccgga
    39841 gccgtgcagc cggggagtcg ggtcacctgg cgttataaat ttggcggcaa cgagaaccag
    39901 ctcgacggct atgagaaatg gttgttacct cagcttaaac ccgaacaacg ctggtacggt
    39961 ctggaacagg acgaaggcgc gctggggcga tcgatggaac gctcgcaaca gttcctgctg
    40021 ctttcggcgc ttctgacctt gctgctggca gtggcagcgg tggcggtagc gatgaatcat
    40081 tactgtcgca gtcgctacga tctggtggcg atcctcaaaa cgctgggggc agggcgagcg
    40141 caactgcgta agctaatcgt cggtcagtgg ttgatggtgc tgacgctttc agccgttacc
    40201 ggtggggcca taggtctgtt gttcgaaaac gtgttgatgg tgctgctcaa gcccgttctg
    40261 cctgctgcac taccgccagc cagcctctgg ccgtggctgt gggcgcttgg caccatgacg
    40321 gtcatctcgc tgctggtggg gctacgacca tatcgcttgt tgctggcaac gcagccttta
    40381 cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc tgaagtttta tctgccgatt
    40441 gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg gtggcagcat gctgctttgg
    40501 gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg gtgtgctggg ctggatgctg
    40561 ctgaatgtac ttcgccgcat aacgctgaaa tcgctgcctc tgcgcctggc ggttagccgc
    40621 ctgttacgtc aaccgtggtc aacgttaagt cagctttcgg cattttcgct ctcctttatg
    40681 ctgctggcgc tgttgctggt attgcgtggc gatctgctcg accgctggca gcagcagcta
    40741 ccgccagaaa gcccgaacta ctttttaatt aacatcgcca cagaacaggt agcaccgcta
    40801 aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat tttatccggt agtgcgggcg
    40861 cggctgacgg cgattaacga taagccgaca gaaggtaatg aagatgaggc gcttaatcgc
    40921 gaactcaatc ttacctggca aaatacgcgg cccgatcata atccgattgt cgccggtaac
    40981 tggccgccaa aagccgatga agtgtcgatg gaagaggggc tggcgaaacg cttaaacgtt
    41041 gccctcggtg ataccgtgac ttttatgggc gatacccagg agttccgcgc taaagtgacc
    41101 agcctgcgca aagtggactg ggaaagtctg cggcctaatt tctattttat tttccctgaa
    41161 ggggcattag acgggcaacc gcagagctgg cttaccagtt tccgctggga gaatggcaac
    41221 ggcatgttga cgcaactcaa ccgccagttc ccgaccatta gcctgttaga cattggcgcg
    41281 attttaaaac aggtcggtca ggtgctggag caggtaagtc gggcgctgga ggtgatggtg
    41341 gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac aggtgcaggt gggaatgcgc
    41401 cagcgtcatc aggagctggt ggtgtggcgc acactcggtg cgggtaaaaa gctgctgcgc
    41461 acgacattgt ggtgcgagtt cgccatgctt gggtttgttt ctggcctggt ggccgcaatt
    41521 ggtgcggaaa cggcactggc agtgttgcag gcgaaagtgt ttgattttcc gtgggagcca
    41581 gactggcgat tgtggattgt tctgccgtgc agcggtgcgc tgctgctgtc gcttttcggc
    41641 ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt tcaggcagtt tgcggggtga
    41701 tgacaggcgt tagattgcct gatgcgctac gcttatcagg cctacgaaga tcgtgcaata
    41761 tattgatttt gcaagattgt gtaggccaga taaggtgttt acgccgcatc cggcatgaac
    41821 agtgtgcatt ttggcaataa atctgaaacc aactgtcatg tcggtttttt taaaggccga
    41881 aggtggtaaa ccttcggcct tagctacgtt tcctcagtta ttcaataaac tgacggatca
    41941 gttccagcag cgcgattagc gccttaagaa cgatgagaac tttatcgaga aaagttttca
    42001 tccgttgctc ccttaaggga gcaagacaat cacagcttac ccttacgttg cctgtaacta
    42061 tgattttctt catgtgctag atgaggactg cctctctcca aattagagca ccagaatcag
    42121 caccacctgt atcctgacca ggacttaaaa gtactttacc gcactgcggt aatcccttga
    42181 ggcagtatca ctactagcgt cgggattgta tcgccaacat agctgtttga atatgtaggc
    42241 atcctgactt ataattcact tgctagatga ggactgtttc atttctgccc ggaaccacca
    42301 agtcttgggc ggaaaaaata aaaaaaccga ctgtcatgtc ggttttttta tatctgtatt
    42361 ctggtgcaac cattctgttc tcacgacaat cgccgtttca gaataccgcc catcactgcg
    42421 ccttaaccca ttccgccact tccgcccact caccgcgaaa gacaactttt tccgcttttt
    42481 tctcaagctg atagcgatac atcgggtcgt aatattcttc aagtaacggc accagccagg
    42541 ccagatgacc gtcggtgctg ccggtggtga gttgcgttgt cagtgctgca tccagccttg
    42601 cagccagttc gttatatcgc tgtagcccca gccgacgctt aatagccgaa agtccgtgat
    42661 gcaggtattc gcaatattcc tgccagccct gttcgtcgcc gtacgcgtgg gtaaaatcat
    42721 gatgcatacg caagaaatac tcttcgttca ggcgctcaag acggatctca aacggatctt
    42781 ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag gcattccggc aggtgattcg
    42841 aaccgatcat ccggctttcg tcttccagca cccacaggcg caaattctga cgggcgtcgg
    42901 tttttagcat ttcggcagcc agcaggtttt caaaactcgc ctggctaagt tgtggttgta
    42961 acgtgcgacc aaacgccgaa ccgcgatgac gcgccaaccc ttccagatca acaccgttcg
    43021 gctgttgctg cactaacagc gttttaccgc tgccggtaca accgccaatc agcactatcg
    43081 gtttttgtgc cagttcaata gtcgcctgaa tcgcggtctg gcgcagtgcc ttataaccgc
    43141 cttccaccag cggataatca atccccgctg catgcaacca gctttgcaca atatgtgagc
    43201 gctgaccgcc acgggcgcag cagagaatac cttgcggatt ttgcaggcac gctgcccgcc
    43261 aggcgtccat gcgctgctga cgaatttcac ccgcaaccag tttatgtccc agcgccagcg
    43321 ctgcgtctga gccttgctgt ttatagcagg tgccaacggc ggcgcgttca tcgttattca
    43381 ttaacggcag attgatagcg gcgggcattg cgccttgctc aaactcgata ggggcgcgaa
    43441 catcaataat gggcgtatca gcaatcagca gggcacgata gtcctgttcc gtgtgtctct
    43501 cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc tattttacgc gccaacgcgc
    43561 aaggaaactt gatttttaac tgcgtgggtt gccgaaaatt tctaaaaatc cgctgatttc
    43621 cggcctgcgc tgggtaaaca aggtcacaat atcttctacc gctttgccgc tgccaaattt
    43681 gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt gggattaccc ggctgaccag
    43741 ttgttgatta tcgatcattg actggcaaag cgattttggc aaaaaaccaa tgccaacgcc
    43801 cgccagatgg gcggcgattt tcgtttccat atcgggaaca ataatctctt tttgccctgg
    43861 caatcgccag gcgacgcgtt tggttaaggt gcgggcgctg tcttcaatat tgaccgccgg
    43921 aaagcgccgc aactgcgctt ctgttagcgg ctcttcaacg ttcgccagcg gatgatccgc
    43981 cgccatgaca aatcgccatt gcaccgatcc taagggatca agactaaagg tatttgccag
    44041 cgcctcagtt cccgtgacgc cgatagccag cgaaaaacct tcgtacaata gcgagtccca
    44101 gacgcccata tagatttgtc gggagatgtg aaactgggta aaggggtaac gctcattcag
    44161 ccacgccagc aactgggcga cggcctgggg gttgtagagc aggttgttga tgacaatatt
    44221 cacctggcgt tccacgccat cattcacctg ttgcagctcg cttggcatac tttccagcca
    44281 gctcagccag tctctggcct gggaaagtag atgctcgcca gccgctgtca acgtcacgct
    44341 gcgagtcgta cggaaaaaca gcgctactcc ggtattctct tccagaagtt taatgcgata
    44401 actgatcgtc gccgtggttt tacataatcg ttctgccgct tttgaaaaac ttcctgtttc
    44461 agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac atcttcaggt atcccctttt
    44521 aaatccgcaa gttgcgtgat tttcttatcc tctgatttat cagtattttt acatgataac
    44581 cctgttcaat ttgtggacta aatctagttt tggaaaaata ttccaacttt tgtattgatg
    44641 ttgttctctt aaggttttag attgcctgtt attgaaacca agctgaccgg tcggcggtgg
    44701 ttgaacggaa ttatgttaca aggacaaaaa gatgaaactt caggtattac cgttaagtca
    44761 ggaagccttt agtgcttatg gcgacgtaat cgaaacgcag caacgggatt ttttccatat
    44821 taacaatggc ctggtggagc gttaccacga tttggcgctg gttgagattc ttgagcaaga
    44881 ccgtacgctt atcagcatta accgcgcgca accggcgaat ctgccgctga ccattcacga
    44941 actcgaacgt catccgctgg gtactcaggc ctttatcccg atgaaaggtg aggtgtttgt
    45001 ggtggtcgtg gcgttaggtg acgacaaacc agacctgtca acgctgcggg cgtttatcac
    45061 caacggcgaa cagggagtga attaccatcg taacgtctgg catcacccac ttttcgcctg
    45121 gcagcgcgtc accgattttc tgaccatcga tcgcggcggc agtgacaact gtgatgttga
    45181 aagtattcct gaacaggaac tctgttttgc gtgacgcctg caaccgactt gcataagata
    45241 aactaattgt tcattgttta tgctcacttg taggtcggag ttaacgtagg tatgacggaa
    45301 gttagacggc gcggcaggcc aggacaggcg gagcctgtgg cacagaaggg cgcacaggca
    45361 ttagagcggg gaattgcgat tctgcaatat ctggaaaaaa gtgggggaag ttcgtcggtt
    45421 agcgatattt ctctcaatct ggatttgccg ctctccacga cctttcgctt gctgaaggtt
    45481 ttacaggcag cggattttgt ctatcaggac agtcagttag gctggtggca tataggatta
    45541 ggtgtcttta acgtcggtgc ggcgtacatc cataaccgcg atgttctctc cgtcgccggg
    45601 ccgtttatgc gccgcctgat gttactttcc ggcgaaacgg tcaatgtcgc gattcgtaac
    45661 ggcaatgaag cagtattaat tggtcagctg gagtgtaaat cgatggtcag gatgtgtgcg
    45721 ccactgggca gtcgtctgcc gctgcatgct tccggtgcgg gcaaggcgtt gctttatccg
    45781 ctggcggaag aggagttgat gagcatcatt ctgcaaaccg gtttgcagca gtttacgcca
    45841 acgacgcttg tggatatgcc caccttgcta aaggacctgg aacaagcgcg tgaactgggc
    45901 tataccgtag ataaagaaga gcatgttgta ggcctgaatt gcatagcttc agcaatttac
    45961 gatgatgtag gtagtgttgt tgccgctatc tccatctccg ggccttcatc aagactgaca
    46021 gaagatcgtt ttgtcagtca gggtgagctg gtcagagaca ccgcccgcga tatcagcacg
    46081 gcgttgggac tgaaagcaca tccatagtgt ctgtcgcatc ccgctctgcg gagcgggttt
    46141 ttttgacaaa atttgaaagt tggaaaattt ttccaattaa tagaggtagg aagaaaatgg
    46201 caaaaatgag agccgttgac gcggcaatgt atgtgctgga gaaagaaggt atcaccaccg
    46261 ccttcggcgt tccgggagct gcaatcaatc cgttctactc agcgatgcgt aagcacggcg
    46321 gtattcgtca cattctggcg cgtcatgtgg aaggtgcttc gcacatggcg gaaggttata
    46381 cccgcgcaat ggcagggaat atcggcgtat gtctggggac ttccggtcct gcgggcacgg
    46441 acatgatcac cgcgctctat tccgcttctg ctgattccat tcctattctg tgtattaccg
    46501 gccaggctcc gcgcgcccgt ctgcataaag aagatttcca ggctgttgat atcgaagcta
    46561 tcgccaagcc ggtaagtaaa atggcggtta cagtacgtga agcggcgctg gtgcctcgtg
    46621 tgctgcaaca ggcatttcac ctgatgcgtt ctggtcgtcc gggtccggta ctggtggatt
    46681 taccgttcga cgttcaggtt gcggaaatcg agtttgatcc tgacatgtac gaaccgttgc
    46741 cggtttacaa accagcagcc agccgtatgc agatcgaaaa agctattgaa atgcttctcc
    46801 aggctgaacg tccggtgatt gttgccgggg gcggggtaat taatgctgac gcagctgcac
    46861 tgttacaaca gttcgctgaa ctgaccagcg ttccggtgat cccaacgctg atgggctggg
    46921 gctgtatccc ggacgatcac gaactgatgg ccgggatggt gggtctgcaa accgcgcatc
    46981 gttacggtaa cgcaacgttg ctggcttccg acatggtatt tggtatcggt aaccgttttg
    47041 ccaaccgtca taccggttcg gtagagaaat acaccgaagg gcgcaaaatc gttcatatcg
    47101 atattgagcc gacgcaaatt ggccgcgtgc tgtgcccgga tctggggatt gtctctgatg
    47161 ctaaagcggc cctgacgctg ctggttgaag ttgcgcagga aatgcaaaaa gcagggcgtc
    47221 tgccatgccg taaagagtgg gttgctgagt gccagcaacg caaacgtact ttgttgcgta
    47281 aaacacactt cgacaacgtt ccggtgaaac cgcagcgcgt gtatgaagag atgaacaaag
    47341 cctttggacg tgatgtttgc tatgtcacca ccattggtct gtcacaaatc gctgcggcac
    47401 aaatgctgca tgtctttaaa gaccgccact ggatcaactg tggtcaggct ggtccgttag
    47461 gctggacgat tccggcggcg ctgggggttt gtgccgctga tccgaaacgc aatgtggtgg
    47521 cgatttctgg cgactttgac ttccagttcc tgattgaaga gttagctgtt ggcgcgcagt
    47581 tcaacattcc gtacatccat gtgctggtca acaacgctta tctggggctg attcgccagt
    47641 cgcagcgcgc gtttgatatg gactactgcg tgcaactcgc tttcgagaat atcaactcca
    47701 gtgaagtgaa tggctacggt gttgaccacg taaaagtagc ggaaggttta ggttgtaaag
    47761 ctattcgggt cttcaaaccg gaagatattg cgccagcctt tgaacaggcg aaagccttaa
    47821 tggcgcaata tcgggtaccg gtagtcgtgg aagttattct cgagcgtgtg actaatattt
    47881 cgatgggcag cgaactggat aacgtcatgg aatttgaaga tatcgccgat aacgcagcgg
    47941 atgcaccgac tgaaacctgc ttcatgcact atgaataagg gagataaata atgttacgtt
    48001 tctctgctaa tttatcgatg ttatttggtg aatatgattt tttcgcccgt tttgagaaag
    48061 ctgcgcagtg tggttttcgc ggcgttgaat ttatgtttcc ttatgactac gacattgaag
    48121 aattaaaaca ggtactggcg agtaataaac tcgaacatac gctgcacaat ttaccggcag
    48181 gtgactgggc ggcgggcgag cgcggtattg cctgtattcc tggccgtgaa gaagagtttc
    48241 gggatggcgt agcagcagcg attcgttatg cccgtgcgct gggtaataaa aaaattaact
    48301 gtctggtcgg taaaacgccg gctggtttca gcagtgaaca gattcacgca acgcttgtag
    48361 aaaacctgcg ttatgccgcg aatatgctga tgaaagaaga tattttatta ctgattgaac
    48421 ctattaacca ttttgatatt cctggtttcc atctcaccgg aactcggcag gcgctgaaat
    48481 tgattgatga tgttggttgc tgcaatttga aaattcagta tgacatttat cacatgcagc
    48541 ggatggaagg tgaattaacc aacactatga cgcaatgggc tgataaaatt ggtcacctgc
    48601 aaattgccga taatccgcat cgcggcgaac cggggaccgg agaaattaat tatgattatc
    48661 tctttaaggt aatcgaaaat tctgactaca acggttgggt tggttgtgaa tataaacccc
    48721 aaaccaccac ggaagccggg ttacgctgga tggatccgta ccgttaaaac gtaacgctgt
    48781 tcaggcaatg cttttttagg ccgctaagtt ggcaggggat cgtgttgtct gaatccagga
    48841 aaagcaaaat ttaaaagagg ttaattatga aactgggatt tattggctta ggcattatgg
    48901 gtacaccgat ggccattaat ctggcgcgtg ccggtcatca attacatgtc acgaccattg
    48961 gaccggttgc tgatgaatta ctgtcactgg gtgccgtcag tgttgaaact gctcgccagg
    49021 taacggaagc atcggacatc atttttatta tggtgccaga tacaccacag gttgaagaag
    49081 ttctgttcgg tgaaaatggt tgtaccaaag cctcgctgaa gggcaaaacc attgttgata
    49141 tgagctccat ttccccgatt gaaactaaac gtttcgctcg tcaggtgaat gaactgggtg
    49201 gcgattatct cgatgcgcca gtctccggcg gtgaaatcgg cgcgcgtgaa gggacgttgt
    49261 caattatggt tggcggtgac gaagcggtat ttgaacgcgt gaaaccgctg tttgaactgc
    49321 tcggtaaaaa tatcaccctc gtgggcggta acggcgacgg tcaaacctgc aaagtggcaa
    49381 atcagattat tgtggcgctc aatattgaag cggtttctga agccctgctg tttgcttcaa
    49441 aagccggtgc agatccggta cgtgtgcgcc aggcgctgat gggcggcttt gcttcctcac
    49501 gtattctgga agttcacggt gagcgcatga ttaaacgcac ctttaatcca ggtttcaaaa
    49561 ttgcgctgca ccagaaagat ctcaacctgg cactgcaaag tgcgaaagcg ctagcgctga
    49621 acctgccaaa cactgcgacc tgccaggagc tatttaatac ctgtgcggca aacggtggca
    49681 gccagttgga tcactctgcg ttagtgcagg cgctggaatt aatggcgaat cataaactgg
    49741 cctgatgccc gcaataaaaa tggccgatat cagcaaatga atcggccagc aatattaaaa
    49801 aaagaaagca gccaaagatg ttgcttcagc attaaaaata atatttttat tttatttgtt
    49861 cctcatagct agattaaaac aacgttattc gatacgtgaa attaagaggg atttatggaa
    49921 catcagagaa aactattcca gcaacgcggc tatagcgaag atctattgcc gaaaacgcaa
    49981 agccagcgga cctggaaaac atttaactat tttaccttat ggatgggttc ggttcataac
    50041 gttcccaatt atgtgatggt cggcggcttt tttattctcg gcttgtctac ctttagtatt
    50101 atgctggcaa ttatcctcag cgcctttttc attgccgcgg taatggtatt aaacggcgct
    50161 gcgggcagta aatacggcgt accgtttgcc atgatcctgc gtgcttctta cggcgtacgt
    50221 ggcgcactgt ttcccggatt attaagaggc ggtattgctg ccattatgtg gtttggcctg
    50281 caatgttatg cggggtcact ggcctgcttg attctgattg gcaaaatctg gccgggattt
    50341 ttaactctcg gtggtgattt cacgctgtta gggctttctc taccgggctt aattactttc
    50401 ttactcttct ggctggtcaa cgttggtatt ggttttggcg gtggcaaagt tttaaataaa
    50461 ttcactgcca ttcttaaccc gtgcatctat atcgttttcg gcggtatggc gatttgggcg
    50521 atttcgctgg tcgggatcgg tccaatcttt gactacattc cgagcggtat tcagaaagca
    50581 gaaaacagtg gcttcctgtt cctggtggtg ataaacgcgg tagttgcggt ctgggcggca
    50641 ccggcggtga gcgcatccga ctttacgcaa aacgcccact cgtttcgtga gcaagcactg
    50701 gggcaaacgc tgggtttggt cgtagcttat attctgtttg cggtcgccgg ggtatgtatt
    50761 attgccggag ccagtattca ctacggcgct gacacatgga acgtgctgga tattgttcag
    50821 cgttgggaca gcctgtttgc ctcgttcttt gcggtactgg ttattctgat gacgaccatt
    50881 tccactaacg ccaccggtaa tattattccg gccggttatc agattgccgc cattgcaccg
    50941 acaaaactga cctataaaaa cggcgtactg attgccagta ttatcagcct gctgatctgc
    51001 ccgtggaaat taatggaaaa tcaggacagt atttatctgt tcctcgatat catcggcggg
    51061 atgctgggtc cggtaattgg tgtcatgatg gcgcattatt ttgtggtgat gcgtggacaa
    51121 attaatcttg atgaactgta taccgcagca ggcgatttca aatattacga taacggcttt
    51181 aaccttaccg cgttctcagt aactttggtc gccgttattt tatctctcgg tggtaaattt
    51241 attccgttca tggagccttt atcacgcgtt tcatggtttg tcggcgttat tgtcgcattc
    51301 gctgcctacg cgttattaaa aaaacgtaca gcagcagaaa aaacaggaga acaaaaagtc
    51361 acaggttaat taatcccgat attgaacatt gagttaaaaa accaatctgt attttacaag
    51421 gagtttgtta tgtcttttga tttaatcatt aaaaacggca ccgttatttt agaaaacgaa
    51481 gctcgcgttg tagatatcgc cgttaaaggc ggaaaaattg ctgctatcgg tcaggatctg
    51541 ggcgatgcaa aagaagttat ggatgcgtct ggtctggtgg tttcgccggg catggttgat
    51601 gcgcacaccc atatttctga accgggtcgt agccactggg aaggttatga aaccggtact
    51661 cgcgcagcgg caaaaggtgg tatcaccacc atgatcgaaa tgccgctcaa ccagctgcct
    51721 gcaacggttg accgcgcttc aattgaactg aagttcgatg ccgctaaagg caagctaact
    51781 atcgatgcgg cacaactcgg tggcctggtg tcttacaaca tcgaccgtct gcatgagctg
    51841 gatgaagtgg gcgttgtcgg cttcaaatgc ttcgttgcga cctgtggcga tcgcggtatc
    51901 gacaacgact tccgtgatgt aaacgactgg cagttcttca aaggtgcgca gaagctgggc
    51961 gaactggggc agccggtgct ggtgcactgc gaaaacgcgc tgatctgtga tgcactgggc
    52021 gaagaagcga aaagtgaagg tcgcgtaact gcccatgact atgtggcttc gcgtccggta
    52081 tttaccgaag tggaagcgat tcgccgcgta ctgtacctgg cgaaagttgc cggttgccgt
    52141 ctgcacattt gccatatcag cagcccagaa ggtgttgaag aagtgactcg tgcacgtcag
    52201 gaaggtcagg atgttacttg tgaatcctgc ccgcattact ttgtactgga taccgatcag
    52261 ttcgaagaaa tcggtactct ggcgaagtgt tcaccgccga tccgcgatct ggaaaaccag
    52321 aaaggcatgt gggaaaaact gtttaacggt gaaatcgact gcctggtttc cgaccactca
    52381 ccatgccctc cggaaatgaa agccggcaac atcatggaag catggggcgg tattgccggt
    52441 ctgcaaaact gtatggacgt gatgttcgat gaagcggtac agaaacgcgg aatgtctctg
    52501 ccaatgttcg gcaaattaat ggcgactaac gcagcagata ttttcggtct gcagcaaaaa
    52561 ggccgtatcg ccccaggaaa agatgccgac ttcgtcttca ttcagccgaa tagcagctat
    52621 gttcttacca atgacgatct ggaatatcgc cacaaagtca gcccgtatgt tggccgtacc
    52681 attggcgcgc gtatcacgaa aaccatctta cgtggtgatg tgatttacga cattgaacag
    52741 ggcttccctg ttgcgccgaa aggtcaattt atccttaaac atcagcagta atctggcccc
    52801 tgcaatgccc gtccttgcgg cgggcattct ccggttaagg tgtgtttatg ttcaattttg
    52861 cagtcagccg cgaaagcctg ttatcaggat ttcagtggtt tttctttatt ttttgcaaca
    52921 cggttgtggt tcctcctacg ctactttctg cttttcagtt gccgcaaagt agcctgctta
    52981 cgctcacgca atatgctttt cttgctaccg cactggcctg cttcgctcag gcgttctgcg
    53041 gtcatcgtcg cgctattatg gaagggccgg gtggcctgtg gtggggaacc atccttacta
    53101 tcacccttgg cgaagcatcg cgcgggacac cgatcaacga tatcgctacc agtctggcgg
    53161 tggggattgc actctccggc gtgctgacga tgttgattgg ttttagcgga ttaggccatc
    53221 gcctggcacg gttatttacg ccgtcggtga tggtcttgtt tatgttgatg ctgggcgcgc
    53281 agctgaccac tatctttttc aaaggtatgc tcgggctgcc gtttggcata gccgacccaa
    53341 attttaaaat tcagttacca ccgttcgcgc tctcggtggc ggtgatgtgc ctggtactgg
    53401 cgatgattat ctttctgccg caacgttttg cccgttatgg cctgctggtc ggtacgataa
    53461 ccggctggtt gttgtggtac ttttgctttc cttcttcgca ttcgctctcc ggtgagttgc
    53521 actggcagtg gttcccgctc ggcagtggcg gtgctttgtc gccgggaatt attctgacgg
    53581 cggtgattac aggtctggtg aatatcagca atacctacgg tgcgattcgg ggcacggatg
    53641 ttttttatcc gcagcagggc gcggggaata cgcgttatcg tcgtagcttt gtggcgaccg
    53701 gatttatgac gctgataacc gtaccgctgg cggtaattcc attttcaccg tttgtttcat
    53761 ccattggttt attaacccag actggcgatt acacgcggcg ttcgtttatt tatggcagcg
    53821 ttatttgcct gctggtggcg ctggttccgg cactcacacg actgttttgc agtatccctt
    53881 tacccgtgag tagcgcggtc atgctggttt cttatctgcc tttactcttt tccgcgctgg
    53941 tgtttagcca gcaaataacc tttaccgctc gcaatattta tcgactcgca ttgccgttat
    54001 ttgtcggcat atttttaatg gcattaccgc ctgtgtatct gcaagacctt ccattaacgc
    54061 tgcgtcctct gctcagtaac ggcttattgg tcgggatttt actggctgtt cttatggata
    54121 atcttattcc gtgggaacgc atcgaataat ttgttgaaaa aggattgata atgaagattg
    54181 tcattgcgcc agactctttt aaagagagct taagtgcaga aaaatgttgc caggcaatta
    54241 aagccgggtt ttcgaccctc tttcccgatg cgcactatat ctgtttgccg atagcggatg
    54301 gcggcgaagg aacggtggat gcgatggtcg ccgcgacggg cggcaacatc gtgacgcttg
    54361 aagtctgcgg gccgatgggc gaaacagtga atgcttttta tggccttacc ggcgacggga
    54421 aaacggcggt gattgagatg gcagcggcaa gtggcctgat gctggtcgcg cctgaaaagc
    54481 gtaatccgtt gctggcctcc agttttggta cgggggagtt aattcgtcat gcgctggata
    54541 acggcattcg ccatattatt ctcggcattg gcggcagtgc gacggtcgac ggcggtatgg
    54601 gcatggcgca ggcgctcggt gtgcgtttcc ttgatgccga cggtcaggtg ctggtggcta
    54661 acggcggtaa tttagcgcgc gtggcaagca ttgagatgga tgaatgcgat ccgcgtctgg
    54721 cgaattgcca tattgaagta gcatgtgacg ttgataaccc gctggtaggg gcacgcggcg
    54781 cggcggcggt gtttggcccg caaaaagggg caacgccgga aatggtcgaa gagctggaac
    54841 aggggctgca aaattacgcc cgtgttttac aacagcaaac tgaaatcaac gtctgccaga
    54901 tggcgggcgg cggcgctgcg ggcggtatgg gtattgcggc ggcggtattt ctcaatgcgg
    54961 atattaaacc aggtattgag attgtgttga atgcggtcaa tcttgcgcag gcagtgcagg
    55021 gcgcagcact ggtgattacc ggggaagggc gcatcgactc gcaaacggca ggcggtaaag
    55081 cgccgctggg tgtggcgtcg gtggcgaagc agtttaatgt accggtgatt gggattgctg
    55141 gcgtattggg tgatggcgtg gaagtggtgc accagtacgg cattgacgcg gtattcagca
    55201 ttttgcctcg tctggcacct ttagccgaag tgctcgccag cggtgaaacc aatctcttca
    55261 acagcgcgcg aaatattgcc tgcgccatta aaataggtca gggaattaaa aactgaccct
    55321 tacctttaaa gcggatgcga tttatatcgc ataagagtgc agtactcatg ccggatgcgg
    55381 catgagtacc atatccttcc tgaaaatcgc gcaaattcta tatattgcag agatcatgta
    55441 ggcctgataa gcgaagcgca tcaggcaatg ttacaaaaaa agccacggta taaaccgtgg
    55501 caaaatccaa catagctaaa aataatcagg cgagtggtat gacttaaatc tctacgtcgc
    55561 ggttgcaatc tttcgagtaa atatagctga acgcttcacc acgccctacg ccataaccag
    55621 cctgtaaaga ataagcgccc ataaagatgt aatcgccttt tttcaccggg atccagctat
    55681 tgtcgaggtt ataaaccccc tgaccggaaa gaatataggc accgtgttcc tgaacgtgtg
    55741 tttcgatata accgtggctg gcacctggtg caaaagagag gatatgcatg ttcatatcaa
    55801 aacctaactc tttgggcaga aaatccagca ggatcacatc atccatacct tcatagtgaa
    55861 tgcgttccag ttcgctggca ttgccagaaa ccagccacgg tgcatggcct tctaccggaa
    55921 catagcggcg cttatataaa aagatttggc tgtcttcggc ctgggcgtta acaaacgtca
    55981 ttaaggagcc tggcgggcaa taaagatagc caccttcgct taaggcaaat gttttgcctt
    56041 cggctttggc agtgatattt ccagagatca catacaggaa cgtttcaatg ccttcgccac
    56101 cgaagccctg ttggttgccg ccgttttgat gcagtgtgac cagataatca acaaaagagg
    56161 cacccagctt tggcgtggag aggattgtcg cgtcacaatt ttcaaagccc ggaataatat
    56221 tttttaccag accgtctggt gttaacagtg cgaaattacc gtgtttcaca atcgcacggt
    56281 tagccagtaa atcatcgcgg taaccggtga cgttatttaa atatcccatt tatgactcct
    56341 tatttctgcc aggcaagttg ataaagcatg agtgccaacg ttttgacccc ttcggcaagg
    56401 tcggtaatat tggtgcgttc cgccgggtta tggctgatcc cgttgatgct gggaatgaaa
    56461 atcatgcagg ttggtacgcg aggcgcgaaa atttgcgcgt catgcccggc accactgtgc
    56521 atcacccggt aattcagttt ttcactttca cacaattctg tcagggtggc gaccaggtcc
    56581 ttattcatcg gcacgggttc ttcgtccatc cataaatcga tatcaatacc aatgtccatt
    56641 tcatcgcaaa tcgcccgcat gtcgttttct aactgttggg tgaaatcacg caggatggcg
    56701 gcgtcggtat gacgacaatc aatggtgaac gtggttttac ccggcaccac attcaccgta
    56761 ttcgggcgcg gctctacttt gccaaaggtc agcaccagcg ggtcgcccat ctttttcgct
    56821 ttttcgatcg actgatggca aatgcgactg aaagcgtaaa ctgtatcacg acggtaaccc
    56881 atcggcgtgg tgcctgcatg gtttgattcg ccgttcagcg tcaccgtata gcgacgttgc
    56941 ccgacaattg cattcaccac gccaattgat tgcccattac tttccagcac acagccctgt
    57001 tcgatatgca gttcgacaaa ggctttaata tcctgacgcg gagttagcgg ggcgtccggc
    57061 agagtaaatc cgcaagcctt catcgcatcg acaaaactat ttcctttggc atcacagata
    57121 ttccgcacgt cgtcaggatt cgccagccca aagatatttt tactgcccca gaaaacatac
    57181 gggaagcggc tgccttcttc ttccgccatc gccaccactt caaccgtacg taacggcgcg
    57241 ccgtattgcg ttttcagcca gtcaattgcc agccacgccg ccagcgcgcc gaattgcccg
    57301 tcgaggttac cgccgttaac cacggtatcg atatgcgaac cgctcagaac cacttcctgt
    57361 ggatattcgg tgccattcag gcgaccgtat aaattcccca cttcatcgaa acgtgtttcc
    57421 agcccgcttg ctgccattct ttttttaaat tgctgctggg tttccagcca ttccggcgaa
    57481 taaagtaaac gggtcatccc acccgctggg tcagcgccaa atgaggaaag ccagggtagc
    57541 gtttcttcta tggcttgacg aaaatgtgta atcataagaa agtcctgtct caataattat
    57601 tgcgcaaagg gatttttcgt ttcgtatgac gtgttataaa gcgcgtcgga aattaaatac
    57661 tggtaaatat catcaacaat ttcgatgcct tcgacggcgg ctttgcgttg tttaatatcc
    57721 tgatcctgtc cgggataata aacctgatta aaaccgggcg cgggggtaat ggcatttaat
    57781 tcgcgcatgg tctggctaag atgttgacgg aataattcgc tggaggagaa aaagttcggg
    57841 ttaataacta catgtaattg ccccaaatta cgcccggcgt gtaaatcgtc atacatcgaa
    57901 ctaacctgtc gcccgaacgg taagccgagt aagacgcctg agaggacgtc aatcatcatc
    57961 atcaggccat accctttcgg cccggcggcg gggagcagag catgtaccgc gaacggatcg
    58021 gttgttggtg caccgttttt atcgaccgcc caggtatccg ggatagacat attacgcgag
    58081 cgggcgtcga gcacttttcc ccatgcctgt acggtagttg ccatatcaaa ggtaaggatc
    58141 tcgtcgcctt ctcccggcgc ggcaaaggcc agtgggttag taccgtagta aatttccgcg
    58201 ccgccaaacg gcaccaccat tggatcggac tgacacatcg aaatgccaat taatccggcg
    58261 tgggctgcct gctgcacaaa ataagagatt gcgccgctgt gacccatccg gctgataccg
    58321 accaccgcaa cgcctttttg ctgggcggtt ttgatggcat gttccatacc cattttcgcc
    58381 gcgacctgtc cggcagcatt gtcggcatgt aaaattgccg agcacggccc ggtttcctca
    58441 agacgaaact ccggttcgcg gttggtgccg ccttttgaaa tgcgttccgc gtagtattcc
    58501 acgcgcaccg cgccatgaga gtggatccct ctggcatcgg cgtaaaccaa tacttcagcc
    58561 acggttgcag cgtgctcacg ttttaaccca gcctggcaga gtttattctc aattagctgg
    58621 tggagtgttt cccgactgat tttcatctgt cttccttttt aacgacggtg tgaagcatga
    58681 ctgcaattaa catacaggga aaatatctgg attatgtgat ccagacaggc aaaaaaatat
    58741 agttagaatt tatttgataa tccgctcgct tttaacctgg tttttaaaac aaccacgctt
    58801 attaaaaaat aacacgtaat agcatggcgg ttatttgaat tcttttgtta ataatccctg
    58861 tgtgatattc atcaccttat ttacccgttg tcatcgatac cgtaatcgcc acattaacac
    58921 tgctcgtgca attgccatgg gtgcaatttt taaggagttg ttatgatcca cgcctttatt
    58981 aaaaaagggt gttttcagga ttcggtcagt ttaatgatta tttcacgaaa actcagcgaa
    59041 tcagaaaatg ttgatgatgt ttccgtaatg atgggtacgc ccgccaataa agcgttatta
    59101 gataccacag gtttctggca tgacgatttt aataacgcca cgccgaacga tatttgcgtg
    59161 gcaattcgta gcgaagcggc ggatgcgggg atcgcgcagg cgattatgca gcagcttgaa
    59221 gaggcgctaa aacaactggc gcagggggca ggcagcagcc aggcgttgac gcaggtgcgt
    59281 cgctgggaca gtgcctgtca gaaattaccc gacgccagtc tggcgctgat ttcagtggct
    59341 ggtgagtatg cggcggagct ggcgaaccag gcgctggatc gcaacctcaa cgtgatgatg
    59401 ttctccgata acgtcacgct ggaagatgaa atccaactta aaacccgcgc gcgggaaaaa
    59461 ggcttgctgg tgatggggcc ggactgcggt acatcgatga ttgccggcac gccgctggct
    59521 tttgctaacg tgatgccgga aggcaacatt ggcgtcattg gcgcttccgg taccgggatt
    59581 caggagctgt gctcgcagat tgcgctggca ggggagggaa ttactcacgc gattggcctt
    59641 ggcgggcgcg acctcagccg tgaagtaggc ggcatcagtg cgctgacggc gctggaaatg
    59701 ctcagtgcag acgagaaaag cgaagtgctg gcatttgttt caaaaccgcc tgccgaagct
    59761 gtgcgtctga aaattgttaa tgccatgaaa gcaaccggca aaccgacggt ggcgctgttt
    59821 ttaggttata ccccggcggt ggcccgcgac gagaatgtct ggtttgcctc ctcgctggat
    59881 gaggccgcac gcctggcttg cctgctttca cgtgtcacgg cgcgacgtaa cgcaatagcg
    59941 cctgtcagca gcggatttat ttgcggtttg tataccggcg gtacgctggc tgccgaagcg
    60001 gcgggattac ttgctggaca ccttggcgtg gaagccgacg atacccatca tcacggcatg
    60061 atgctcgacg ccgatggtca ccagattatt gacctcggcg atgatttcta caccgtcggg
    60121 cgtccccatc cgatgatcga cccagcctta cgcaatcagt taattgccga tctcggcgct
    60181 aaaccgcaag tgcgcgtatt gctgcttgat gtcgtgattg gcttcggtgc gaccgccgat
    60241 cctgccgcct cgctggtaag cgcctggcaa aaagcatgtg ccgcgcgttc agacaatcaa
    60301 ccactgtatg ccattgccac ggtgacaggc actgaacgtg acccgcaatg ccgctcgcag
    60361 caaatcgcca cgctggaaga tgcagggatc gcggtagtga gttcgctgcc ggaagccacc
    60421 ttgctggcgg cggcactgat tcgtccgctt tcgcctgcca ctcagcaaca cacaccgtca
    60481 ttactggaaa acgtcgccgt gattaacatc ggattacgca gctttgcgct ggagctacaa
    60541 agcgccagca aaccggttgt gcattaccaa tggtcgccag tcgccggcgg caataaaaaa
    60601 ctggctcgtt tattagaacg tttgcaataa ggggttccca tgtttacatc agtagcgcaa
    60661 gccaatgctg cggttatcga acaaattcgt cgcgctcgtc cacactggct ggatgtgcaa
    60721 ccggcttctt cacttatcag cgaactaaac gagggcaaaa cactgcttca cgccgggccg
    60781 ccaatgcgct ggcaggagat gaccggaccc atgaaagggg cgtgcgtggg cgcatgtctg
    60841 ttcgaaggct gggcgaaaga tgaagcgcag gcgctggcaa tactggagca gggggaagtg
    60901 aacttcattc cttgtcacca tgtgaatgcc gtcggtccaa tgggcggtat tacttctgcc
    60961 agtatgccga tgctggtggt cgagaacgtg accgacggca accgggcgta ctgcaacctc
    61021 aacgaaggta tcggcaaagt gatgcgtttt ggcgcttacg gcgaagatgt cctgactcgc
    61081 catcgctgga tgcgcgatgt gttaatgcca gtattaagcg cggcgctggg gcgcatggag
    61141 cgcggtatcg atctcacggc gatgatggcg cagggcatta cgatgggcga tgagttccat
    61201 caacgcaata ttgcttcctc tgcactgtta atgcgtgcgc tggccccaca aattgctcgc
    61261 ctcgatcatg ataaacagca catcgccgaa gtgatggatt tcctcagcgt gaccgatcag
    61321 ttcttcctca acctcgcgat ggcttactgc aaggcggcga tggatgctgg cgcgatgatc
    61381 cgcgcaggca gcatcgtcac ggcaatgacc cgcaacggca atatgttcgg gattcgggta
    61441 agcgggctgg gcgaacgctg gtttactgcg cctgtaaaca ctccgcaagg tctgtttttc
    61501 accggcttct cgcaggagca ggcgaacccg gatatgggcg atagcgcgat taccgaaacc
    61561 tttggtatcg gaggtgcggc aatgatcgca gcgcctggcg taacgcgctt tgtcggtgcg
    61621 ggtggcatgg aagcggccag agcggtatct gaagagatgg cggaaattta ccttgagcgc
    61681 aatatgcagt tgcagatccc aggctgggat tttcagggcg cgtgcctggg actggacatt
    61741 cgtcgcgtgg tagaaaccgg cattacgcca ctcatcaata ccggtatcgc ccataaagag
    61801 gcggggatcg ggcagattgg cgcaggcact gtgcgggcac cgctggcgtg ttttgaacag
    61861 gcgctggaag cactggctga aagcatgggg attggttgag gaacgcgcaa tgacgatcat
    61921 ccatcctctg cttgccagta gtagcgcacc gaattatcgc cagtcctggc ggttagcggg
    61981 agtgtggcgg cgggcgatta acctgatgac ggaaagcggc gaactgttaa cgctgcatcg
    62041 tcagggtagt ggtttcggcc ccggaggatg gatgcttcgc cgtgcgcaat tcgatgcgtt
    62101 atgcggtgga ttatgcggca atgaacgacc acaggttgtg gctcaaggga ttcgcctcgg
    62161 gcgtttcacg gttaaacagc cacagcgtta ttgtttgctg cgtattacgc cgcctgcgca
    62221 tcctcaacca cttgcagctg catggatgca acgcgcggag gaaaccgggc ttttcgggcc
    62281 actggcgttg gcggcaagcg atccgctgcc tgctgagtta cgccagtttc gtcactgttt
    62341 tcaggccgcg ctcaatggcg ttaagaccga ctggcggcac tggctgggta aaggccccgg
    62401 attaacgccg agtcatgatg acacgctgag cggaatgctg ctggcggcct ggtattatgg
    62461 cgctttagat gcgcgctccg gtcgccagtt ttttgcctgt tccgacaatc tgcaactcgt
    62521 taccacagcg gtgagcgtca gttatttacg ttatgccgcg caaggatatt tcgcctcgcc
    62581 actcctgcac tttgttcatg ctctgagttg cccgaaacgt accgctgttg cgattgattc
    62641 gctgctggcg ctggggcata cgtcaggggc agatacactg ctggggttct ggcttggtca
    62701 acaattatta caaggaaaac catgaaaaca ctggttgtag ctcttggggg caatgccttg
    62761 cttcagcgcg gtgaggcgct gacggcagaa aatcaatatc gcaatatcgc cagtgctgta
    62821 cccgcgctgg cgcgcctggc ccgttcttat cggctggcga ttgttcacgg caacgggccg
    62881 caggtggggc tgctggcatt gcagaatctg gcgtggaaag aggtagaacc gtatccgctg
    62941 gatgtgctgg tggcagaaag tcaggggatg attggctata tgctggcgca gagtttgagc
    63001 gcacagccgc agatgccgcc cgtgacgacg gtgctgacgc gcattgaggt ttcgcctgat
    63061 gatccggcgt ttttgcagcc agagaaattt attggtccgg tttatcagcc agaagaacaa
    63121 gaggcactgg aagcggctta cggctggcag atgaaacgtg atggtaaata tttgcgccgg
    63181 gtggtggcgt ctccgcaacc gcgtaaaatt ctcgacagcg aagccatcga gttgttgctc
    63241 aaagaggggc atgtggtgat ttgcagtggc ggcggcggtg tgcctgtgac ggaagatggg
    63301 gcagggagtg aagcggtgat tgataaagat ctcgctgctg cgttgctcgc tgagcagatt
    63361 aacgccgatg ggctggtgat cctcactgat gctgatgcgg tatatgaaaa ctggggaacg
    63421 ccgcagcaac gtgccattcg ccatgccaca ccggatgagt tagcgccatt tgccaaagcc
    63481 gatggttcga tggggccgaa ggtaacggcg gtgagtggct atgtcagaag ccgtggcaaa
    63541 cctgcgtgga ttggggcgtt atcgcggatt gaagagacgc tggcgggcga agcggggacc
    63601 tgtatttcgc tgtagtgatg agagcatccg gcaatcggtg caccatgcct gatgcgacgc
    63661 ttgacgcgtc ttatcaggcc tacaaccggt gccgcatccg gcaattggtg cacaatgcct
    63721 gatgcgatgc ttgacgcatc ttatcaggcc tacaatgggt accggatcgg taggccggat
    63781 aaggcgttta cgccgcatcc ggcaagaata gagcaccagt taaccgaact tactctgcgc
    63841 ccaaatcacg ccgctggcat attccggcgg cagcagcggg attaaggctt ccagcgtcgc
    63901 agtcagacgc gatgtgtcgc tgtcggtcaa attcagatgc cccactttac gccccggacg
    63961 gacttctttg tcgtaccagt gcagatgcac cagcggcaat ttcagccagt cataattcac
    64021 atcgctacca atcagattga tcatcaccga cggactattc actactggtt gcggtaacgg
    64081 cagatcggta atcgcccgca gatgcagctc aaactggctg atgctggcac cgttttgtgt
    64141 ccagtgaccg ctgttatgca cacgcggagc cagttcgttg atcagcagac cttgcggggt
    64201 gacaaaacac tccatcgcca tcacgcccac atagcccagc tcctgcataa tcgccgacag
    64261 catctcttcg gcttgcgcct gctgctgtgc gttggcctgc ggaaaagcga cgctggtgcg
    64321 caaaataccg tcctgatgca ggttatgcgt cagcggataa aacacggtgc tgccatcaaa
    64381 gccgcgcgcg ccaaccagcg acacttcacc agagaagtta atgccctgct cgacaataca
    64441 ttcgccgtaa cactctgccg gtaactgttc ggtttcattt gcgcgtaaac gccattgacc
    64501 gcgaccgtca tagccaccag tgcgacgctt aacaatcgcc agttcaccta aacgatcaaa
    64561 caccgcaggc cactcgctgc gatcggcaag taactgccac ggcgcggtcg gcaggtggag
    64621 cttatcgaaa agctgcttct gagtcagacg gtcagcaata atcgggaaca catcgcggtt
    64681 cacaaaggcc ggatggcgcg ccagctcgcg ggttaatgcg gtttccggcc agcgttctat
    64741 ctcagcggta atcacgcttt gttgaaaagg caccgccgcc ggttcagcgt ccagcccgac
    64801 tggccagaca gcaatgccta acggttcgcc tgcctgacgc agcatacggc ctaactgccc
    64861 gttaccgagg acgcaaacct gtttcatgcc gcacctcgcg ggtccgggtt ttccagcact
    64921 tcgtcggtct gggctttgcg ccagtcattc agacgctggt gcagttcttt atcatgagtc
    64981 gcaagaattt gtgctgccag taacgccgcg tttgccgcgc cagctttacc aatcgccagc
    65041 gtacccaccg gaatgccgcg cggcatttgt acgatggagt agaggctatc gacaccgctc
    65101 agtgcggcgc tctgtactgg cacgcccagc accggcacca gcgttttggc ggcaatcatg
    65161 cctggcagat gcgctgcgcc gcctgcgccc gcaataatca cctgataacc gttctcttcg
    65221 gcgctttcgg cgaagctgaa cagtttatcg ggggtgcggt gagcagaaac cacttcaacg
    65281 tggtgcggga cattcaggat ttcgaagatt tcggcggcga actgcatggt agcccagtcg
    65341 cttttggacc ccatcacgat ggcgacacgc gccggattat tgcgggaaga catgcgtctt
    65401 aaaactcctg tggtgcacaa ctctcggctt tagagggcac agagaatagc acggaaagag
    65461 agcaaggaaa acggttgcgt ggctgtgaaa tcagcaaagt tgcgggtttt ttaaaacgga
    65521 aaatgaatca gctcaacgtc atccgccgtg actttcacca tcgaaccttc tgtatgccag
    65581 gcaccgagta ccacgcgaaa agcaggttgc tggttggcga taagttcatg taccgccggg
    65641 cgatgggtat gcccgtggat cagccattgc acctgatgtt tttccatcgc actgactacc
    65701 gcgttttggt taacgtccat gatcgccagc gatttactgc tgttggcttc tttactgttc
    65761 gctcgcattc gcgcggcaat gcgtttgcgc acaaacaacg gcagggcgag gaatagcgtt
    65821 tgcagccagg gtttgtggac tttggcgcga aaagcctgat aacccgcgtc atcggtgcac
    65881 agcgtgtcgc catgcataat caacacccgg cgaccataaa gttcgagcac cttttcttcc
    65941 ggcaataatg tcatgccgct ttcacgggca aagcgtttgc cgagcagaaa atcacggttg
    66001 ccatgaatga aataacaggg aacgccggaa tcggacaccg ctttgatcgc tgccgccatc
    66061 tggcgatgga gtgggttggg atcgtcgtcg ccaatccatg cttcaaacag atcgccaaga
    66121 atatacagcg cgtcggcctt gcgggcttcc cccgctaaaa aacgcagaaa accggcggtg
    66181 atcgccggtt cttccacgca gagatgaaga tctgcaataa agagtgtcgc cacgattact
    66241 cgctaacggt cacgctttca atgataacgt cttcttttgg cacgtcctgg tgcataccgc
    66301 tacgaccggt tgcaacacct ttgattttgt ctaccacgtc catgccgtca accacttcag
    66361 caaacacgca gtagccccaa ccttgcaggc tttcgccaga gaagttcagg aagtcgttat
    66421 caaccacgtt gatgaagaac tgtgcagttg cagagtgcgg agcctgagta cgtgccattg
    66481 ccagcgtacc acgggtattt ttcaggccgt tgttggcttc gtttttgatc ggttctttgg
    66541 tggctttttg tttcatgccc ggttcaaaac cgccgccctg aatcataaag ccgttgataa
    66601 cacggtggaa aatggtgttg ttgtaaaaac cttcgcggca gtagtccagg aagtttttaa
    66661 ctgtttcagg tgctttatcg tcaaaagttt tgatgacaat atcgccgtga ttggtgtgga
    66721 aagtaaccat ttttgcatcc tgttccgttt gattggtgct tcaacccagt tcgggtcata
    66781 tatagggtgg tgttatagca taaccgcacg atcggatcat cacgcaatgt atgctgattc
    66841 gcgcgggaaa tatgggtatt atacgcaact caattaccca cacatgtcta aacggaatct
    66901 tcgatgctaa aaatcttcaa tactctgaca cgccaaaaag aggaatttaa gcctattcac
    66961 gccggggaag tcggcatgta cgtgtgtgga atcaccgttt acgatctctg tcatatcggt
    67021 cacgggcgta cctttgttgc ttttgacgtg gttgcgcgct atctgcgttt cctcggctat
    67081 aagctgaagt atgtgcgcaa cattaccgat atcgacgaca aaatcatcaa acgcgccaat
    67141 gaaaatggcg aaagctttgt ggcgctggtg gatcgcatga tcgccgaaat gcacaaagat
    67201 tttgatgcgt tgaacattct gcgcccggat atggagccgc gcgcgacgca ccatatcgca
    67261 gaaattattg aactcactga acaactgatc gccaaaggtc acgcttatgt ggcggacaac
    67321 ggcgacgtga tgttcgacgt cccgaccgat ccaacttatg gcgtgctgtc gcgtcaggat
    67381 ctcgaccagc tgcaggcagg cgcgcgcgtt gacgtggtcg acgacaaacg caacccaatg
    67441 gacttcgttc tgtggaagat gtcgaaagag ggcgaaccga gctggccgtc tccgtggggc
    67501 gcgggtcgtc ctggctggca cattgaatgt tcggcaatga actgcaagca gctgggtaac
    67561 cactttgata tccacggcgg cggttcagac ctgatgttcc cgcaccacga aaacgaaatc
    67621 gcgcagtcca cctgtgccca tgatggtcag tatgtgaact actggatgca ctcggggatg
    67681 gtgatggttg accgcgagaa gatgtccaaa tcgctgggta acttctttac cgtgcgcgat
    67741 gtgctgaaat actacgacgc ggaaaccgtg cgttacttcc tgatgtcggg ccactatcgc
    67801 agccagctga actatagcga agagaacctg aagcaggcgc gtgcggcgct ggagcgtctc
    67861 tacactgcgc tgcgcggcac agataaaacc gttgcgcctg ccggtggcga agcgtttgaa
    67921 gcgcgcttta ttgaagcgat ggacgacgat ttcaacaccc cggaagccta ttccgtgctg
    67981 tttgatatgg cgcgtgaagt aaaccgtctg aaagcagaag atatggcagc ggcgaatgca
    68041 atggcatctc acctgcgtaa actttccgcc gtattgggcc tgctggagca agaaccggaa
    68101 gcgttcctgc aaagcggcgc gcaggcagac gacagcgaag tggctgagat tgaagcgtta
    68161 attcaacagc gtctggatgc ccgtaaagcg aaagactggg cggcggcaga tgcggcgcgt
    68221 gaccgtctta atgagatggg gatcgtgctg gaagatggcc cgcaagggac cacctggcgt
    68281 cgtaagtaat tgcgctattg ccggatgcga gttttcgcat ccggttatcg tctgcgccac
    68341 cacaacattc ccatcagtag catccccggc aaccacaccc acatcaattc agaaataatc
    68401 acctgatgcc cgtacgctgt ggtgtaacga gacaatgcaa acggcgcgac tttaatcacc
    68461 tgccagggag cgaaaaagcg ttcatctgac cacggccaca gccagccaac ccctttaccg
    68521 ccagtggtta ccgaatccag caagctgtgc gatagcaacg agacggttaa aaacagccag
    68581 cagcgaatca gcccagccct gaaccatcga cgtccaatca gcacacataa cagcgggaca
    68641 acaaacgcaa acaccagcga atgggtaaac ccgcgatgac caaaaacatt gccgtaagca
    68701 acgccaaaat taaacgacaa gacgtcggca tcgggcagca tcgccaggat gattccggca
    68761 aataacaggc gcggagggat gactttcgaa cccaacccta aaccaatgca taggggaacg
    68821 gcggcgtgcg taataacggt tggcatgatg gtcgcttcgg caaaatgtcg atgctatcag
    68881 catggatgaa cggggcgtag agggcaaaag tctgaaaaga gaaccggcct gttgatacag
    68941 gccgggaaag ggatcaggca acaacctgta cgctgtgacc tgcaaaactc actgtctgac
    69001 cggcgacgat tttgcagcgt ttgcgcgttt caaccgcacc gtcgactttc acctggcctt
    69061 cggcaatcgc gattttcgcc tgcgcgccgc tttcgctcca gccttccagt ttcagcaagt
    69121 cgcacagctc aacgtgcgga tgtttaccta aagaaaatgt cgccatgtta ctcatcctgt
    69181 ggatcatgat attcaacgca cgcctgtagc gtgttttcaa tcagcgtggc aaccgtcatc
    69241 gggccaacgc cgccgggaac aggcgtaatg tatgaggcgc gtttagccgc gtcttcaaac
    69301 acgacgtcgc ccacaacttt gccattttcc agacggttga tgccgacatc aatcacaatt
    69361 gcgccttctt tgatccagtc accgggaata aagcctggct tgccaacggc aacgatcaat
    69421 agatcggcat tttctacgtg atgacgcaga tttttagtga agcggtgagt cactgtagtg
    69481 gtgcaacctg ccagcagcag ttccatgctc atcgggcggc caacgatatt cgatgcgcca
    69541 atcaccacgg cgttgaggcc gaaggtatca atgttgtaac gctcaagcag cgtgacgata
    69601 ccgcgcgggg tgcagggacg cagacgcggc gcgcgctggc acagacgacc gacgttgtaa
    69661 ggatggaaac cgtccacgtc tttgtccgga tgaatacgtt ccagcacttt gacgttatca
    69721 atacccgccg gtaacggcag ttgaaccaga atgccatcga tggtgttgtc ggcattcagc
    69781 gtatcgataa gctccagcag ctccgcttcg ctggtggttt ccgggaggtc ataagagcgg
    69841 gagacgaacc cgacttcttc acaagccttg cgtttgcttg cgacataaat ttgcgatgca
    69901 gggttactac ccaccagcac aacggccagt cctggtgccc gcagtccggc tgcaatacgc
    69961 gcctgaactt tttgagcaac ttcagagcgc acctgctgcg caatcgtttt accgtcaata
    70021 atctttgctg ccatcagaga gaggattcca tctgttacgt aggtcgaagg ggatgcgcct
    70081 attttgtcag aagcggggcg cgctgtcagg tttcgtttca gatttatcgc gtgaagcgac
    70141 ctcttgcgaa ggtgaggcgc accgtcgctg agactgaaag cttcattttt cgtccatgat
    70201 ggcgttgtaa atctggaact gatttatttc cttgtctaag gattaagata atttaagaaa
    70261 tacctgacaa tataaaaaga attttcagcc tggtaattta c
//
LOCUS       DAJOSY010000010        65526 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJOSY010000010 DAJOSY010000000
VERSION     DAJOSY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.010"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(219..1031)
                     /gene="yidA"
                     /locus_tag="OHN27_000716"
     CDS             complement(219..1031)
                     /gene="yidA"
                     /locus_tag="OHN27_000716"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCQ2285214.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(1146..1544)
                     /locus_tag="OHN27_000717"
     CDS             complement(1146..1544)
                     /locus_tag="OHN27_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCQ2285215.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(1784..4198)
                     /gene="gyrB"
                     /locus_tag="OHN27_000718"
     CDS             complement(1784..4198)
                     /gene="gyrB"
                     /locus_tag="OHN27_000718"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCQ2285216.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(4227..5300)
                     /gene="recF"
                     /locus_tag="OHN27_000719"
     CDS             complement(4227..5300)
                     /gene="recF"
                     /locus_tag="OHN27_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCQ2285217.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(5300..6400)
                     /gene="dnaN"
                     /locus_tag="OHN27_000720"
     CDS             complement(5300..6400)
                     /gene="dnaN"
                     /locus_tag="OHN27_000720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCQ2285218.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(6405..7808)
                     /gene="dnaA"
                     /locus_tag="OHN27_000721"
     CDS             complement(6405..7808)
                     /gene="dnaA"
                     /locus_tag="OHN27_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCQ2285219.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            8105..8185
                     /locus_tag="OHN27_000722"
     CDS             8105..8185
                     /locus_tag="OHN27_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285220.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            8415..8555
                     /gene="rpmH"
                     /locus_tag="OHN27_000723"
     CDS             8415..8555
                     /gene="rpmH"
                     /locus_tag="OHN27_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCQ2285221.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            8572..8931
                     /gene="rnpA"
                     /locus_tag="OHN27_000724"
     CDS             8572..8931
                     /gene="rnpA"
                     /locus_tag="OHN27_000724"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCQ2285222.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8895..9152
                     /gene="yidD"
                     /locus_tag="OHN27_000725"
     CDS             8895..9152
                     /gene="yidD"
                     /locus_tag="OHN27_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCQ2285223.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            9155..10801
                     /gene="yidC"
                     /locus_tag="OHN27_000726"
     CDS             9155..10801
                     /gene="yidC"
                     /locus_tag="OHN27_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCQ2285224.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10907..12271
                     /gene="mnmE"
                     /locus_tag="OHN27_000727"
     CDS             10907..12271
                     /gene="mnmE"
                     /locus_tag="OHN27_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCQ2285225.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            12514..12588
                     /gene="tnaC"
                     /locus_tag="OHN27_000728"
     CDS             12514..12588
                     /gene="tnaC"
                     /locus_tag="OHN27_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCQ2285226.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            12809..14224
                     /gene="tnaA"
                     /locus_tag="OHN27_000729"
     CDS             12809..14224
                     /gene="tnaA"
                     /locus_tag="OHN27_000729"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCQ2285227.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            14315..15562
                     /gene="tnaB"
                     /locus_tag="OHN27_000730"
     CDS             14315..15562
                     /gene="tnaB"
                     /locus_tag="OHN27_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCQ2285228.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWLSSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            15694..16869
                     /gene="mdtL"
                     /locus_tag="OHN27_000731"
     CDS             15694..16869
                     /gene="mdtL"
                     /locus_tag="OHN27_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCQ2285229.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            16844..17803
                     /gene="yidZ"
                     /locus_tag="OHN27_000732"
     CDS             16844..17803
                     /gene="yidZ"
                     /locus_tag="OHN27_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCQ2285230.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRINVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHQTWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            17960..18709
                     /locus_tag="OHN27_000733"
     CDS             17960..18709
                     /locus_tag="OHN27_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCQ2285231.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLQPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            18731..19297
                     /gene="yieF"
                     /locus_tag="OHN27_000734"
                     /gene_synonym="chrR"
     CDS             18731..19297
                     /gene="yieF"
                     /locus_tag="OHN27_000734"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCQ2285232.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(19351..20688)
                     /gene="adeP"
                     /locus_tag="OHN27_000735"
     CDS             complement(19351..20688)
                     /gene="adeP"
                     /locus_tag="OHN27_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCQ2285233.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAESFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            20855..21520
                     /gene="yieH"
                     /locus_tag="OHN27_000736"
     CDS             20855..21520
                     /gene="yieH"
                     /locus_tag="OHN27_000736"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCQ2285234.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            21587..22060
                     /locus_tag="OHN27_000737"
     CDS             21587..22060
                     /locus_tag="OHN27_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCQ2285235.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            22109..22696
                     /locus_tag="OHN27_000738"
                     /pseudo
     CDS             22109..22696
                     /locus_tag="OHN27_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
     gene            complement(22758..23480)
                     /locus_tag="OHN27_000739"
     CDS             complement(22758..23480)
                     /locus_tag="OHN27_000739"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCQ2285236.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGASKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(23495..24664)
                     /locus_tag="OHN27_000740"
     CDS             complement(23495..24664)
                     /locus_tag="OHN27_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCQ2285237.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(24691..26307)
                     /gene="bglH"
                     /locus_tag="OHN27_000741"
     CDS             complement(24691..26307)
                     /gene="bglH"
                     /locus_tag="OHN27_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCQ2285238.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENYMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(26376..27788)
                     /gene="bglB"
                     /locus_tag="OHN27_000742"
     CDS             complement(26376..27788)
                     /gene="bglB"
                     /locus_tag="OHN27_000742"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCQ2285239.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKKITIKAP"
     gene            complement(27807..29684)
                     /gene="bglF"
                     /locus_tag="OHN27_000743"
     CDS             complement(27807..29684)
                     /gene="bglF"
                     /locus_tag="OHN27_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCQ2285240.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTSLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(29818..30654)
                     /gene="bglG"
                     /locus_tag="OHN27_000744"
     CDS             complement(29818..30654)
                     /gene="bglG"
                     /locus_tag="OHN27_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCQ2285241.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(30940..31665)
                     /gene="phoU"
                     /locus_tag="OHN27_000745"
     CDS             complement(30940..31665)
                     /gene="phoU"
                     /locus_tag="OHN27_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCQ2285242.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(31680..32453)
                     /gene="pstB"
                     /locus_tag="OHN27_000746"
     CDS             complement(31680..32453)
                     /gene="pstB"
                     /locus_tag="OHN27_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCQ2285243.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(32636..33526)
                     /gene="pstA"
                     /locus_tag="OHN27_000747"
     CDS             complement(32636..33526)
                     /gene="pstA"
                     /locus_tag="OHN27_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCQ2285244.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(33526..34485)
                     /gene="pstC"
                     /locus_tag="OHN27_000748"
     CDS             complement(33526..34485)
                     /gene="pstC"
                     /locus_tag="OHN27_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCQ2285245.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(34572..35612)
                     /gene="pstS"
                     /locus_tag="OHN27_000749"
     CDS             complement(34572..35612)
                     /gene="pstS"
                     /locus_tag="OHN27_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCQ2285246.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPDLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILVHKDQKKPEQGVEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(35926..37755)
                     /gene="glmS"
                     /locus_tag="OHN27_000750"
     CDS             complement(35926..37755)
                     /gene="glmS"
                     /locus_tag="OHN27_000750"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCQ2285247.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(37917..39287)
                     /gene="glmU"
                     /locus_tag="OHN27_000751"
     CDS             complement(37917..39287)
                     /gene="glmU"
                     /locus_tag="OHN27_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCQ2285248.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(39638..40057)
                     /gene="atpC"
                     /locus_tag="OHN27_000752"
     CDS             complement(39638..40057)
                     /gene="atpC"
                     /locus_tag="OHN27_000752"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCQ2285249.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(40078..41460)
                     /gene="atpD"
                     /locus_tag="OHN27_000753"
     CDS             complement(40078..41460)
                     /gene="atpD"
                     /locus_tag="OHN27_000753"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCQ2285250.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(41487..42350)
                     /gene="atpG"
                     /locus_tag="OHN27_000754"
     CDS             complement(41487..42350)
                     /gene="atpG"
                     /locus_tag="OHN27_000754"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCQ2285251.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(42401..43942)
                     /gene="atpA"
                     /locus_tag="OHN27_000755"
     CDS             complement(42401..43942)
                     /gene="atpA"
                     /locus_tag="OHN27_000755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCQ2285252.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(43955..44488)
                     /gene="atpH"
                     /locus_tag="OHN27_000756"
     CDS             complement(43955..44488)
                     /gene="atpH"
                     /locus_tag="OHN27_000756"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCQ2285253.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFVAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(44503..44973)
                     /gene="atpF"
                     /locus_tag="OHN27_000757"
     CDS             complement(44503..44973)
                     /gene="atpF"
                     /locus_tag="OHN27_000757"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCQ2285254.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(45035..45274)
                     /gene="atpE"
                     /locus_tag="OHN27_000758"
     CDS             complement(45035..45274)
                     /gene="atpE"
                     /locus_tag="OHN27_000758"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCQ2285255.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(45321..46136)
                     /gene="atpB"
                     /locus_tag="OHN27_000759"
     CDS             complement(45321..46136)
                     /gene="atpB"
                     /locus_tag="OHN27_000759"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCQ2285256.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(46145..46525)
                     /gene="atpI"
                     /locus_tag="OHN27_000760"
     CDS             complement(46145..46525)
                     /gene="atpI"
                     /locus_tag="OHN27_000760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCQ2285257.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(47143..47766)
                     /gene="rsmG"
                     /locus_tag="OHN27_000761"
     CDS             complement(47143..47766)
                     /gene="rsmG"
                     /locus_tag="OHN27_000761"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCQ2285258.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(47830..49719)
                     /gene="mnmG"
                     /locus_tag="OHN27_000762"
     CDS             complement(47830..49719)
                     /gene="mnmG"
                     /locus_tag="OHN27_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCQ2285259.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(50098..50541)
                     /gene="mioC"
                     /locus_tag="OHN27_000763"
     CDS             complement(50098..50541)
                     /gene="mioC"
                     /locus_tag="OHN27_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCQ2285260.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     PASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWVNLLK"
     gene            complement(50631..51089)
                     /gene="asnC"
                     /locus_tag="OHN27_000764"
     CDS             complement(50631..51089)
                     /gene="asnC"
                     /locus_tag="OHN27_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCQ2285261.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            51241..52233
                     /gene="asnA"
                     /locus_tag="OHN27_000765"
     CDS             51241..52233
                     /gene="asnA"
                     /locus_tag="OHN27_000765"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCQ2285262.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(52238..53689)
                     /gene="viaA"
                     /locus_tag="OHN27_000766"
     CDS             complement(52238..53689)
                     /gene="viaA"
                     /locus_tag="OHN27_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCQ2285263.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(53683..55179)
                     /gene="ravA"
                     /locus_tag="OHN27_000767"
     CDS             complement(53683..55179)
                     /gene="ravA"
                     /locus_tag="OHN27_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCQ2285264.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            55402..57270
                     /gene="kup"
                     /locus_tag="OHN27_000768"
     CDS             55402..57270
                     /gene="kup"
                     /locus_tag="OHN27_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCQ2285265.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            57437..57856
                     /gene="rbsD"
                     /locus_tag="OHN27_000769"
     CDS             57437..57856
                     /gene="rbsD"
                     /locus_tag="OHN27_000769"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCQ2285266.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            57864..59369
                     /gene="rbsA"
                     /locus_tag="OHN27_000770"
     CDS             57864..59369
                     /gene="rbsA"
                     /locus_tag="OHN27_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCQ2285267.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVNRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            59374..60339
                     /gene="rbsC"
                     /locus_tag="OHN27_000771"
     CDS             59374..60339
                     /gene="rbsC"
                     /locus_tag="OHN27_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCQ2285268.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            60364..61254
                     /gene="rbsB"
                     /locus_tag="OHN27_000772"
     CDS             60364..61254
                     /gene="rbsB"
                     /locus_tag="OHN27_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HCQ2285269.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            61380..62309
                     /gene="rbsK"
                     /locus_tag="OHN27_000773"
     CDS             61380..62309
                     /gene="rbsK"
                     /locus_tag="OHN27_000773"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCQ2285270.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            62313..63305
                     /gene="rbsR"
                     /locus_tag="OHN27_000774"
     CDS             62313..63305
                     /gene="rbsR"
                     /locus_tag="OHN27_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCQ2285271.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(63271..64698)
                     /gene="mdtD"
                     /locus_tag="OHN27_000775"
     CDS             complement(63271..64698)
                     /gene="mdtD"
                     /locus_tag="OHN27_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCQ2285272.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(64721..65413)
                     /locus_tag="OHN27_000776"
     CDS             complement(64721..65413)
                     /locus_tag="OHN27_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCQ2285273.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT        15848 a        16936 c        16410 g        16332 t
ORIGIN      
        1 attactcaaa ggtgatggcg ttctaccatc catttaaagt gcgattttat gaatactatt
       61 cacccagagg cattccggat ggtgtttccg cagcattact gccagtgact gttacgctgg
      121 acatcattac tgcaccgctg caatttctgg ttgtatatgc agtaaaccaa taatcagtaa
      181 gcgggcaaac gcgtttatgc tgtttgcccg cccacagatt aattcagcac atacttctca
      241 atagcaaacg ccacgccatc ttcaaggtta gatttggtga caaagttcgc cacttctttc
      301 actgaaggaa tagcgttatc catcgccaca ccgacgcctg catattcaat cattgcgata
      361 tcgttttcct gatcgccaat cgccatgatt tcttccggtt taatacctaa cacgtcggcc
      421 agtgatttca cccccgtacc tttgttaacg cgtttatcga ggatttcgag gaagtacggc
      481 gcacttttca gcacggtata tttctctttc acttcctgcg gaatacgcgc gatagcctgg
      541 tcgaggatgg cgggttcatc aatcatcatc actttcagga actgggtatt ggggtccatt
      601 ttctccgctt cgcagaacac cagcggaatt gtggcaacga aggattcatg caccgtgtag
      661 tagctgatat cacggttggc ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa
      721 ccgacttcgc gagagagttt ttccaggaaa cggtagtcgt catagctgag agcggtttgc
      781 gccacggtgc taccatcagc ggccttctgt accagcgcgc cgttataagt aatgcagtag
      841 tcgcccggca gttccatatg cagctctttc aggtaattgt gaacacctgc ataagggcga
      901 cccgtcgtta gcacgacatt cacgccacgg gcgcgagctg cggcaatcgc atttttaacg
      961 gcgggtgaaa tggtgtgatc gggtagcaga agggtgccat ccatatcgat agcaatgagt
     1021 ttgatagcca tgagttcccc aggtagatta gttcctgacc catgctaacg cgattccgct
     1081 caaaaatcag tacaacaccc gagggaaaag ggggatgcaa cgcgggttgc atgctcccca
     1141 tttgcttagc ggaagagttt ccctttcagc agttccatgc ctgcggaaag cagatcgtta
     1201 ttggcttgtg gtgacacttc accttgcggt gagagcgcat caataatctt cggcaattgc
     1261 tctgccagta aactggaagc tgtactggta tccacgccaa gtttttgccc gagatcggac
     1321 accgcatttg tgccgagcgc cgattccagt tgctcgccac taaccgattg attgccctgt
     1381 tgattactca gccaggttga gagaatggcc cctaagccgc cactttgcag tttttccagc
     1441 agcacctgaa tgccgccctg ctcctcaacc caacttaaaa tagcctgata tttccccgca
     1501 tcgcctttca gaaaggcacc gacaacttca tcaaaaagcc ccatgataat cacctgtaaa
     1561 gcgttacgtg ttgacccaaa aagtatagat ttgcgggatg ataattgcgg attgcagaaa
     1621 taaaaagggc ggagatgatc tccgcccttt tcttatagtt tcttgccgga tgcggcgtga
     1681 acgccttatc cggcctacaa aatcatgaaa attcaataca ttgcaagatt ttcgtaggcc
     1741 tgataagcgt agcgcatcag gcacgctcgc atggttagcg ccattaaata tcgatattcg
     1801 ccgctttcag ggcgttctct tcaataaacg cacggcgcgg ttcaacggcg tcgcccatca
     1861 gcgtggtgaa caactggtcg gcagcaatcg catctttaac ggtaacgcgc agcatacgac
     1921 gactttccgg gtccatagtg gtttcccaca gctgttccgg gttcatctcg cccagacctt
     1981 tataacgctg gatggagagg ccgcgacggg actctttcac cagccagtcc agcgcctgct
     2041 cgaagctggc taccggctga cgacgctcgc cacgttcgat aaacgcatct tcttccagca
     2101 agccacgcag tttctcaccc agcgtgcaga tacgacgata ttcgccaccg gtgataaact
     2161 cgtgatccag cggatagtca gtatccacac cgtgggtacg cacgcgaaca atcggctcga
     2221 acaggttttg ttcggcattg gtgtggacat caaacttcca ctggctgccg tgctgttctt
     2281 tgtcgttcag ttcgctgacc agcgcgttca cccagcgggt aacggtctgc tcatcagaga
     2341 ggtcggcttc cgtcagcgtc ggctgataga taagctcttt cagcattgct ttcggataac
     2401 gacgctccat gcggttgatc attttctgcg tcgcgttgta ctcagacacc agtttctcta
     2461 acgcttcgcc agccaatgcc ggtgcgctgg cgttggtgtg cagcgttgcg ccgtccagcg
     2521 cgatagagat ctggtactga tccatcgctt cgtcgtcttt aatgtactgt tcctgcttgc
     2581 ctttcttcac tttgtacagc ggcggctgag cgatgtagac gtgaccacgt tcaacgattt
     2641 ccggcatctg acgatagaag aaggtcaaca gcagcgtacg aatgtgcgag ccgtcgacgt
     2701 ccgcatcggt catgatgatg atgctgtgat aacgcagttt gtccgggttg tactcgtcac
     2761 gaccgatacc acagccaagc gcggtgataa gcgtcgccac ttcctgagaa gagagcatct
     2821 tatcgaagcg cgctttctcg acgttgagga ttttaccctt cagcggcaga atcgcctggt
     2881 tcttgcggtt acgcccctgc ttcgcagagc cgcccgcgga gtccccttcc accaggtaca
     2941 gttcggaaag cgccggatcg cgttcctggc agtctgccag tttgcccggc aggcccgcta
     3001 agtcgagcgc acctttacgg cgggtcattt cacgcgcgcg acgcgcagct tcacgggcac
     3061 gggcagcatc gataattttg ccaactacga ttttcgcgtc ggttgggttt tccagcaggt
     3121 attctgccag cagttcgttc atctgctgtt caaccgccga tttcacctca gaagaaacca
     3181 gtttgtcttt ggtctgggag gagaatttcg ggtccggcac tttcacggaa acgaccgcaa
     3241 tcaagccttc acgcgcatcg tcaccggtgg cgctgacttt ggcttttttg ctgtagcctt
     3301 ctttgtccat gtaggcattc agggtacggg tcatcgccgc acggaagcct gccaggtgag
     3361 taccgccgtc acgctgcgga atattgttgg taaagcagta gatgttttcc tggaagccat
     3421 cgttccactg cagcgccact tcaacgccaa taccgtcttt ttcagtggag aagtagaaga
     3481 tattcgggtg aatcggcgtt ttgttcttgt ttagatattc aacgaacgcc ttgatgccgc
     3541 cttcatagtg gaagtggtct tctttgccgt cgcgcttgtc gcgcagacga atggaaacgc
     3601 cggagttgag gaacgacaac tcacgcagac gtttcgccag aatttcatat tcgaactcgg
     3661 tcacattggt gaaggtttcg aggctgggcc agaaacgcac catggtgccg gttttttcag
     3721 tctcgccggt aaccgccagc ggggcctgcg gtacaccgtg ttcgtagatc tgacggtgaa
     3781 ttttaccctc gcgctggata accagctcca gtttttgcga cagggcgttt actaccgaaa
     3841 caccaacgcc gtgcagaccg ccggacactt tataggagtt atcgtcaaat ttaccgcctg
     3901 cgtgcagaac ggtcatgatc acttccgccg ccgatacgcc ctcttccggg tgaataccgg
     3961 tcggaatgcc gcgcccgtca tcctgtacag agacagagtt atcggcgtga atggtgacga
     4021 taatttcttt acagtgaccc gcgagcgctt cgtcgatagc gttatctacc acctcgaata
     4081 ccatgtggtg cagaccggtg ccgtcatccg tgtcgccgat atacataccc gggcgcttac
     4141 gcaccgcatc cagccctttc aggactttga tactggagga gtcataagaa ttcgacatca
     4201 acgtttctcg ctcatttata cttgggttaa tccgttattt tacccttttc cacggtaaac
     4261 atcttcgaat tttcgtccga catgtctata acgtgttcag cactgatcgc gctgacaaag
     4321 acctgtgatt gcgtcgcttt taagcggctg gcaagcagcc cgcgacgctc atcatcaagc
     4381 tcagaggcaa aatcatctat caggtagaga caccgccgcc cgctttcacg ggtgaggaac
     4441 tctccttgcg ccagacgtaa ggcgcacatc aacagcttaa gctgcccacg cgataaggta
     4501 tcttccaccg gcgcaccgtc ggcgcgaatg cgtaagtccg ctttgtgcgg gccgtgcgcg
     4561 gtgtaggtta gctggcgatc gcgttcaaaa ttacgttcca gcacctcagc atattctgtc
     4621 tctttctccc agccgcgctg gaaagagaaa gtcagagaaa actcagggag aaattgctta
     4681 caggtatcag ccatatcggc cgcgataccg gcgctatact ccgcgcgcca ggtgctgatt
     4741 tgctccgcca gcgggatcag ctctttatcc cacgggcgta gctgttcgta acgtgtcacc
     4801 tggcgcagcg ccgcattgcg ctgcttgagc aatcgcttga gattgctcca ggcggtgaaa
     4861 aatccgggtt cgttgtgaaa gcatccccag tcgaggaatg ctcttctgta tttggggccg
     4921 ccgttgagta aagtaaaccc ttctggcgtt atcaactgca ttggcatcag gtgcgccagt
     4981 tccgcgacct tatgcccgtc tgtaccgtcg atgcggactt tgctgtcgcc ctgtttgtct
     5041 ttggttaagc caatcgctgt ctcgcgctct tcgccctgta atcgcccgtg gagaacaaac
     5101 gcctcctgct catggcgaat gacgcgacca atctgcaaac tgcgaaacgc ccgaccatgg
     5161 ccgagcgtat agatggcttc cagcacgctg gttttgccac tgccgttggc acctaccaga
     5221 aagttaaagc cgggagataa ggcgagatcc gcggtttcaa tgttgcggaa atcgcggatc
     5281 aacaagcggg tgagggacat tacagtctca ttggcatgac aacataagcc gcgctctggc
     5341 tggccgcatc ttcaatctgc acgctggaaa ccgaatcggt cagcatcatg cggacgtttt
     5401 cgcatttcag cgcgttcaga acatccagca cataactgac gttgaagccg atttccatct
     5461 ccgcaccgct ataggtaacg tcgaggatct cttccgcttc ttcctgttcc gggttgttgg
     5521 cggtgatttt cagctggttt tcgctgacat aaagacgtac gccgcggaat ttctcgttag
     5581 agagaatcgc cgcgcgagca aacgcctgct tgagcagatc gcagccagct tccagatgtt
     5641 tgtccgggtt cttcggcaga acgcggcgat aatccgggaa gcgaccatcc accagtttgg
     5701 aggtgaagat aaagtcgcca acgtgggcgc gaatgttgtt gctgccaatc tgtacgcgca
     5761 gcggattgtc gccgccgtcg agcatacgca tcagttcaat cacgccttta cgcggtacga
     5821 tcaccgaatg gcttggcaaa gattgaccaa ttggcattga acagaccgcc agacggtggc
     5881 cgtcggttgc cacggtgcgc agttcttcac cttcggtttc aaacagcata ccatttaagt
     5941 aatagcgaac gtcctgatgc gccatagaaa actgggtcgc ttcaatcaga cgcttcatcg
     6001 ttgcctgcgg cagggtaaat tcgacttcac tctgccagtc atcgaggttc gggaaatccg
     6061 ccgctggcag ggtagacagc gaaaaacggc tacgcccgga gcgtaccagc atccgttcac
     6121 cttccagctg cacggcaatt tccgcgcctt caggcagacc acggcagata tcaaagaatt
     6181 tgcgcgccgg aacggtcgtc gctcctggct cgtgtggctg aaccagcgca acacgtgcca
     6241 ccatttccat ctcgagatca gtaccggtca gcgacaacgt accgtcagca acctgtaaca
     6301 gcagattacc gagaatcggt agcgtaggac gaccacctaa cggaccgctc acctgttgta
     6361 gcggttttaa taaatgctca cgttctacgg taaatttcat aggtttacga tgacaatgtt
     6421 ctgattaaat ttgaaaaatc ttctttgata tcgtggctct cttcacgcaa ctgctcgatc
     6481 ttacggcagg catgaagcac cgtcgtgtgg tcacgaccac caaacgcatc gccaatctcc
     6541 ggcagactgt ggttagtcag ctctttcgcc agcgccatcg ccatctggcg cggacgcgcc
     6601 accgagcggg atcgacgctt ggaaaggaga tccgcgactt tgatcttgta gtactccgcc
     6661 accgtcttct gaatattgtc gatggtgacc agtttttcct gcaatgccag caagtcgcgc
     6721 agcgcctcac gcacgaagtc gatggtgatc gcccgtccgg taaagttggc attggcaatg
     6781 acgcggttca gcgccccttc cagctcacgt acgttagatc gtagacgctt ggcgataaag
     6841 aacgccactt cgcccggcaa acgaatgtcg ttttcgtcgg cctttttcat caggatcgcc
     6901 acacgggttt ccagctctgg cggttcgatc gccacagtca gtccccaacc gaagcgggat
     6961 ttcaaacgat cctcaacgcc gttgatctct ttcggatagc gatccgaggt gagaatgatc
     7021 tgttgattac cttccagcag ggcgttgaag gtgtggaaaa actcttcctg agatcgttct
     7081 ttattagcaa aaaactgaat atcgtcgatc agcagtgcat ctacggaacg gtagtagcgt
     7141 ttaaactctt cgatcgcgtt gttttgcagg gctttaacca tgtcctgaac aaagcgctcg
     7201 gagtgcatat aaaccacttt ggcattcggc ttgcgcgcca taatgccgtt acccaccgca
     7261 tgcagcaggt gagttttacc cagacccgtg ccgccataaa ggaacaacgg gttataggca
     7321 ccgccagggt tatccgccac ctggcgagcc gccgcgcgcg ccagttggtt agatttacct
     7381 tcaacgaagt tatcaaacgt gtgtttgacg tttacgttag aacgataggt cggttctgcc
     7441 ggggccggga cgttatccca acctgagcgc gtagaaggcg cagcacgttg cggctgcgtt
     7501 tgcgccacct gtgcaggggc cgcgacgttg ctcgtcactg ccgcttgtgg cgtttgcgtc
     7561 accggtttgg tgccgacttc aaaacgcagc tgtggggcat ccgctccgca gaaactggtt
     7621 agcagtccat tgatattatt aaggtacttg tcccgtaccc aatcgaggac aaaacggttt
     7681 ggcgcgtaca gggccagcgt gttatcgctc agttccgcct gcaatgggcg tatccacata
     7741 ctgaattctg tggctggtaa ctcatcctgc aatcgggcaa gacactgctg ccaaagcgaa
     7801 agtgacacgg cggactccac tcgaacaaaa gtcgataatg actaaggctg aaacattcat
     7861 gattgttgac gtacgtcgaa aagaccctgc ttgagggtga cgcacgaacc gctgtctgcg
     7921 gttatatgcc cgatcaagat cctgcaaaac gatcgggacc gcggatcata gcctaaactg
     7981 cgcaagagat cttctgtttc tcacagattt tcccgattta tccacaggac tttccagaac
     8041 tcgctaagtg taaacgatcc tgccgcgagg cgggcacgat ttacgccgca tattggaaaa
     8101 tttaatgacc atagacaaaa attggcttaa tcgatctaat aaagatccag gacgatcctt
     8161 gcgctttacc catcagcccg tataatcctc cacccggcgc gccatgctgg tttccactgg
     8221 tgtgaggtcg tacattttcc ctgcgaaaag gtgcggaaaa gcgcggtaaa taaggaaaga
     8281 gaattgactc cggagtgtac aattattaca atccggcctc tttaatcacc catggcttcg
     8341 gtgtccatcg tttcattttt cggcggatat ccaataaagc cattgaattt attcaagttt
     8401 aggtagaaat cgccatgaaa cgcacttttc aaccgtctgt actgaagcgc aaccgttctc
     8461 acggcttccg tgctcgtatg gctactaaaa atggtcgtca ggttctggca cgtcgtcgtg
     8521 ctaaaggccg cgctcgtctg accgtttcta agtaataaag ctaacccctg agtggttaag
     8581 ctcgcatttc ccagggagtt acgcttgtta actcccagtc aattcacatt cgtcttccag
     8641 cagccacaac gggctggcac gccgcaaatt accattctcg gccgcctgaa ttcgctgggg
     8701 catccccgta tcggtcttac agtcgccaag aaaaacgttc gacgcgccca tgaacgcaat
     8761 cggattaaac gtctgacgcg tgaaagcttc cgtctgcgcc aacatgaact cccggctatg
     8821 gatttcgtgg tggtggcgaa aaaaggggtt gccgacctcg ataaccgtgc tctctcggaa
     8881 gcgttggaaa aattatggcg ccgccactgt cgcctggctc gcgggtcctg atagccctca
     8941 ttcgggtcta tcaacgcctg attagtccgc tactcgggcc gcattgtcgt ttcactccaa
     9001 cctgttcaag ctacggaatt gaggcattgc gcaggtttgg agtgataaaa ggcagttggt
     9061 tgacggtgaa acgcgtatta aaatgccacc ctttacaccc tggtggtgac gatcccgtcc
     9121 cgcccggacc atttgatacc agagaacact aacgatggat tcgcaacgca atcttttagt
     9181 catcgctttg ctgttcgtgt ctttcatgat ctggcaagcc tgggagcagg ataaaaaccc
     9241 gcaacctcag gcccaacaga ccacgcagac aacgaccacc gcagcgggta gcgccgccga
     9301 ccagggcgta ccggccagtg gccaggggaa actgatctcg gttaagaccg acgtgcttga
     9361 tctgaccatc aacacccgtg gtggtgatgt tgagcaagct ctgctgcctg cttacccgaa
     9421 agagctgaac tctacccagc cgttccagct gttggaaact tcaccgcagt ttatttatca
     9481 ggcacagagc ggtctgaccg gtcgtgatgg cccggataac ccggctaacg gcccgcgtcc
     9541 gctgtataac gttgaaaaag acgcttatgt gctggctgaa ggtcaaaacg aactgcaggt
     9601 gccgatgacg tataccgacg cggcaggcaa cacgtttacc aaaacgtttg tcctgaaacg
     9661 tggtgattac gctgtcaacg tcaactacaa cgtgcagaac gctggcgaga aaccgctgga
     9721 aatctcctcg tttggtcagt tgaagcaatc catcactctg ccaccgcatc tcgataccgg
     9781 aagcagcaac ttcgcactgc acaccttccg tggcgcggcg tactccacgc ctgacgagaa
     9841 gtatgagaaa tacaagttcg ataccattgc cgataacgaa aacctgaaca tctcttcgaa
     9901 aggtggttgg gtggcgatgc tgcaacagta tttcgcgacg gcgtggatcc cgcataacga
     9961 cggtaccaac aacttctata ccgctaatct gggtaacggc atcgccgcta tcggctataa
    10021 atctcagccg gtactggttc agcctggtca gactggcgcg atgaacagca ccctgtgggt
    10081 tggcccggaa atccaggaca aaatggcagc tgttgctccg cacctggatc tgaccgttga
    10141 ttacggttgg ttgtggttca tctctcagcc gctgttcaaa ctgctgaaat ggatccatag
    10201 ctttgtgggt aactggggct tctccattat catcatcacc tttatcgttc gtggcatcat
    10261 gtacccgctg accaaagcgc agtacacctc catggcgaag atgcgtatgt tgcagccgaa
    10321 gattcaggca atgcgtgagc gtctgggcga tgacaaacag cgtatcagcc aggaaatgat
    10381 ggcgctgtac aaagctgaga aggttaaccc gctgggcggc tgcttcccgc tgctgatcca
    10441 gatgccaatc ttcctggcgt tgtactacat gctgatgggt tccgttgaac tgcgtcaggc
    10501 accgtttgca ctgtggatcc acgacctgtc ggcacaggac ccgtactaca tcctgccgat
    10561 cctgatgggc gtaacgatgt tcttcattca gaagatgtcg ccgaccacag tgaccgaccc
    10621 gatgcagcag aagatcatga cctttatgcc ggtcatcttc accgtgttct tcctgtggtt
    10681 cccgtcaggt ctggtgctgt actatatcgt cagcaacctg gtaaccatta ttcagcagca
    10741 gctgatttac cgtggtctgg aaaaacgtgg cctgcatagc cgcgagaaga aaaaatcctg
    10801 attcggtgag ttttcgctaa aataagggcg gtcagttgac cgcctttttt cttttcgtag
    10861 ggcggataag caccgcgcat ccgccacaca aagcaacagg aacatcatga gcgataatga
    10921 cactatcgta gcccaggcca cgcctccggg acgtggcggc gttggcatcc tgcgcatctc
    10981 cggcttcaaa gcccgtgaag ttgccgaaac cgtgctgggt aaactgccta agccgcgcta
    11041 cgccgattat cttccgttta aagacgccga cggcagcgtg ctcgatcagg ggattgcgct
    11101 atggttccct ggcccgaact cgttcaccgg cgaagatgtg ctggaactgc aaggtcatgg
    11161 cggtccggtg atcctcgacc tgctgttaaa acgcattctg accattcccg gcctgcggat
    11221 tgctcgccct ggtgagtttt ccgaacgcgc gtttcttaac gataaacttg acttagccca
    11281 ggccgaggcg attgccgatc ttatcgacgc cagttcggaa caggcggccc gttcggcact
    11341 taactcgctg caaggcgcat tctccgcacg ggttaatcat ctggtagaag ccctcaccca
    11401 cttgcgcatt tacgtcgaag cggcaattga tttccccgat gaagagatcg atttcctctc
    11461 cgacggaaaa attgaagccc agctcaatga cgttattgcc gatcttgatg cagtgcgtgc
    11521 tgaagcacgt cagggtagtt tgttgcgcga agggatgaaa gtggtgattg ccggacgtcc
    11581 taacgccggt aaatcgagcc tgttaaacgc gctggcgggg cgtgaagcgg caatcgtaac
    11641 cgatatcgcc ggaactacgc gtgacgtgct gcgtgagcat atccacattg acggaatgcc
    11701 gctgcatatc atcgataccg ccgggctacg tgaagccagt gacgaagtag aacgtattgg
    11761 tatcgagcgc gcgtggcagg aaattgaaca ggccgaccgc gtgctgttta tggtcgatgg
    11821 caccacaaca gacgccgtgg atccggcaga gatctggccg gaatttattg cccgtctgcc
    11881 agcgaaactg ccgatcaccg tggtgcgcaa taaagccgat atcaccggcg aaacgctggg
    11941 aatgagtgaa gtgaacggtc acgcgttaat tcgtctctcg gcaaggactg gtgaaggcgt
    12001 ggacgtgctg cgtaaccatc tcaaacagag catgggcttt gacaccaaca tggaaggcgg
    12061 cttcctggcg cgtcgtcgcc acctacaggc gctggaacag gcagcggaac atctacaaca
    12121 gggcaaagcg caactgttgg gagcctgggc aggtgaactg ctggcggaag agttgcgtct
    12181 ggcacagcag aacttaagcg aaatcaccgg ggaatttact tcagacgacc tgctggggcg
    12241 gattttctcc agcttctgta ttggtaagta accgcgctta cgaagccgca ttctgactgt
    12301 cagatgcggc ttcgcttcat tgttaccact cctgttattc ctcaaccctt tttttaaaca
    12361 ttaaaattct tacgtaattt ataatcttta aaaaaagcat ttaatattgc tccccgaacg
    12421 attgtgattc gattcacatt taaacaattt cagaatagac aaaaactctg agtgtaataa
    12481 tgtagcctcg tgtcttgcga ggataagtgc attatgaata tcttacatat atgtgtgacc
    12541 tcaaaatggt tcaatattga caacaaaatt gtcgatcacc gcccttgatt tgcccttctg
    12601 tagccatcac cagagccaaa ccgattagat tcaatgtgat ctatttgttt gctatatctt
    12661 aattttgcct tttgcaaagg tcatctctcg tttatttact tgttttagta aatgatggtg
    12721 cttgcatata tatctggcga attaatcggt atagcagatg taatattcac agggatcact
    12781 gtaattaaaa taaatgaagg attatgtaat ggaaaacttt aaacatctcc ctgaaccgtt
    12841 ccgcattcgt gttattgagc cagtaaaacg taccactcgc gcttatcgtg aagaggcaat
    12901 tattaaatcc ggtatgaacc cgttcctgct ggatagcgaa gatgtgttta tcgatttact
    12961 gaccgacagc ggcaccgggg cggtgacgca gagcatgcag gctgcgatga tgcgcggcga
    13021 cgaagcctac agcggcagtc gtagctacta tgcgttagcc gagtcagtga aaaatatctt
    13081 tggttatcaa tacaccattc cgactcacca gggccgtggc gcagagcaaa tctatattcc
    13141 ggtactgatt aaaaaacgcg agcaggaaaa aggcctggat cgcagcaaaa tggtggcgtt
    13201 ctctaactat ttctttgata ccacgcaggg ccatagccag atcaacggct gtaccgtgcg
    13261 taacgtctat atcaaagaag ccttcgatac gggcgtgcgt tacgacttta aaggcaactt
    13321 tgaccttgag ggattagaac gcggtattga agaagttggt ccgaataacg tgccgtatat
    13381 cgttgcaacc atcaccagta actctgcagg tggtcagccg gtttcactgg caaacttaaa
    13441 agcgatgtac agcatcgcga agaaatacga tattccggtg gtaatggact ccgcgcgctt
    13501 tgctgaaaac gcctatttca tcaagcagcg tgaagcagaa tacaaagact ggaccatcga
    13561 gcagatcacc cgcgaaacct acaaatatgc cgatatgctg gcgatgtccg ccaagaaaga
    13621 tgcgatggtg ccgatgggcg gcctgctgtg catgaaagac gacagcttct ttgatgtgta
    13681 caccgagtgc agaacccttt gcgtggtgca ggaaggcttc ccgacatatg gcggcctgga
    13741 aggcggcgcg atggagcgtc tggcggtagg tctgtatgac ggcatgaatc tcgactggct
    13801 ggcttatcgt atcgcgcagg tacagtatct ggtcgatggt ctggaagaga ttggcgttgt
    13861 ctgccagcag gcgggcggtc acgcggcatt cgttgatgcc ggtaaactgt tgccgcatat
    13921 cccggcagac cagttcccgg cacaggcgct ggcctgcgag ctgtataaag tcgccggtat
    13981 ccgtgcggta gaaattggct ctttcctgtt aggccgcgat ccgaaaaccg gtaaacaact
    14041 gccatgcccg gctgaactgc tgcgtttaac cattccgcgc gcaacatata ctcaaacaca
    14101 tatggacttc attattgaag cctttaaaca tgtgaaagag aacgcggcga atattaaagg
    14161 attaaccttt acgtacgaac cgaaagtatt gcgtcacttc accgcaaaac ttaaagaagt
    14221 ttaattaata ctacagagtg gctataagga tgttagccac tctcttaccc tacatcctca
    14281 ataacaaaaa tagccttcct ctaaaggtgg catcatgact gatcaagctg aaaaaaagca
    14341 ctctgcattt tggggtgtta tggttatagc aggtacagta attggtggag gtatgtttgc
    14401 tttacctgtt gatcttgccg gtgcctggtt tttctggggt gcctttatcc ttatcattgc
    14461 ctggttttca atgcttcatt ccgggttatt gttattagaa gcaaatttaa attatcccgt
    14521 cggctccagt tttaacacca tcaccaaaga tttaatcggt aacacctgga acattatcag
    14581 cggtattacc gttgccttcg ttctctatat cctcacttat gcctatatct ctgctaatgg
    14641 tgcgatcatt agtgaaacga tatcaatgaa tttgggttat cacgctaatc cacgtattgt
    14701 cgggatctgc acagccattt tcgttgccag cgtattgtgg ttaagttcgt tagccgccag
    14761 tcgtattacc tcattgttcc tcgggctgaa gattatctcc tttgtgatcg tgtttggttc
    14821 ttttttcttc caggtcgatt actccattct gcgcgacgcc accagctcca ctgcgggaac
    14881 gtcttacttc ccgtatatct ttatggcttt gccggtgtgt ctggcgtcat ttggtttcca
    14941 cggcaatatt cccagcctga ttatttgcta tggaaaacgc aaagataagt taatcaaaag
    15001 cgtggtattt ggttcgctgc tggcgctggt gatttatctc ttctggctct attgcaccat
    15061 ggggaatatt ccgcgagaaa gctttaaggc gattatctcc tcaggcggca acgttgattc
    15121 gctggtgaaa tcgttcctcg gcaccaaaca gcacggcatt atcgagtttt gcctgctggt
    15181 gttctctaac ttagctgttg ccagttcgtt ctttggtgtc acgctggggt tgttcgatta
    15241 tctggcggac ctgtttaaga ttgataactc ccacggcggg cgtttcaaaa ccgtgctgtt
    15301 aaccttcctg ccacctgcgt tgttgtatct gatcttcccg aacggcttta tttacgggat
    15361 cggcggtgcc gggctgtgcg ccaccatctg ggcggtcatt attcccgcag tgcttgcaat
    15421 caaagctcgc aagaagtttc ccaatcagat gttcacggtc tggggcggca atcttattcc
    15481 ggcgattgtc attctctttg gtataaccgt gattttgtgc tggttcggca acgtctttaa
    15541 cgtgttacct aaatttggct aaatccttca agaagccagc cattcgctgg cttcttgcct
    15601 ctcaggaaat cacttatgtc caaatggcaa ctcgcctgat cctccttcac cacgtatgct
    15661 ttgcgtcacc ttactatcag gacgctttag cctatgtccc gctttttgat ttgtagtttt
    15721 gccctggttt tactttatcc cgccgggatt gatatgtacc tcgttggttt accgcgcatc
    15781 gccgccgatc tcaatgccag cgaagcgcag ttgcatattg cgttctccgt atatctggcg
    15841 gggatggcag ctgcgatgtt atttgccggt aaagtggccg atcgttcagg gagaaagccg
    15901 gtcgccatac ccggcgcggc gctatttatt attgcctcgg tgttctgttc actggctgaa
    15961 accagcacgt tatttcttgc aggccgattt ctacaggggt tgggcgcagg ctgttgttac
    16021 gtagtggcgt tcgctatttt gcgcgacacg ctggatgatc gacgtcgggc taaagtgctg
    16081 tcattactca acggtattac ctgcatcatt ccggtgttag cgccagtgct cggacatctg
    16141 attatgctta aattcccgtg gcagagtctg ttctgggcga tggcaatgat gggcatcgcg
    16201 gtactgatgt tgtctttgtt tattttaaaa gaaacgcgcc cagcggcccc cgcagcttcg
    16261 gataaaccac gagaaaatag cgagtcgctg cttaaccgtt ttttcctcag ccgtgttgtt
    16321 atcaccaccc tcagcgtttc ggtgatcctc actttcgtca acacgtcacc ggtattgctg
    16381 atggaaatca tggggtttga gcgcggtgaa tacgccacca ttatggcgct gaccgctggc
    16441 gtcagcatga ccgtttcatt ctccacgcca tttgcgctgg gaatttttaa gccacgtacg
    16501 ttgatgatca cctcgcaggt gttattcctg gcggcgggga tcactcttgc cgtttcacct
    16561 tcccatgcgg tttctctgtt tggtatcacg ctgatttgcg ccggtttctc ggtaggtttt
    16621 ggtgtggcga tgagtcaggc gttagggccg ttttcattac gcgcgggcgt agccagctcg
    16681 accttaggta ttgcgcaggt ttgcggttcg tcactgtgga tttggctggc agcggtggtt
    16741 ggtatcggcg catggaatat gctgatcggg attctgattg cctgtagcat agtgagcctg
    16801 ttgctgatta tgttcgtcgc gcctggacgc cccgttgccg ctcatgaaga aatccatcac
    16861 cacgcttgat ctcaatctgc tgctttgtct gcaactgctg atgcaggagc gcagcgtaac
    16921 caaagcggcg aagcggataa acgtgacacc ttcggcggtg agtaagtcgc tggcaaagtt
    16981 aagagcgtgg tttgacgacc cgctctttgt gaactcaccg ctgggtctgt cgcccacacc
    17041 gctgatggtc agcatggagc aaaatctggc ggagtggatg caaatgagca acctgctgct
    17101 ggataaaccg caccaccaga caccgcgcgg cctgaagttt gagctggcgg cggaatcacc
    17161 gctgatgatg atcatgctta atgcgctgtc gaaacagatc taccaacgtt acccgcaggc
    17221 gaccatcaaa ttacgtaact gggattacga ttccttagat gccattactc gtggtgaagt
    17281 ggatatcggt ttttccggtc gcgaaagcca tcctcgctcg cgggagctgt taagctcgct
    17341 accgttagcc attgattatg aagtgctgtt tagtgatgtg ccctgcgtct ggttacgcaa
    17401 agatcatccg gcactgcatc aaacgtggaa tctggacacc ttcttacgtt atccgcatat
    17461 cagcatttgc tgggaacaga gcgatacctg ggcgctggac aatgtgttac aggagctggg
    17521 acgcgaacgc acgattgcta tgagcctgcc ggaatttgag cagtcactgt ttatggcagc
    17581 gcaacccgac aatctgctac tggcgaccgc gccgcgctac tgtcagtact acaatcaact
    17641 ccatcaactg ccgttggttg ctcttcctct cccgtttgac gaaagccagc aaaaaaagct
    17701 ggaagttcct tttaccctgc tgtggcataa acggaacagc cataatccga agatcgtctg
    17761 gttacgggaa accattaaaa acctttacgc gtcgatggca taaccgaatc gtatgaaacg
    17821 cgacccaatt tcacaataaa atgtaaaaaa gttgtaataa gcttgtctga atcgaacttt
    17881 tagccgcttt agtctgtcca tcattccagt aaatgattac tcttatattc ataatggacc
    17941 attaagcatg gagcgaaaaa tggcgactca ctttgcccga gggattttaa cggaaggaca
    18001 tctgatttct gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca ttccccctca
    18061 tcgtcaaagc cgttttctgg cgtccagagg tttactcgca gaactgatgt tcatgctgta
    18121 tggcattggc gaattgccgg aaatcgtcac cctgccgaaa ggtaaaccgg ttttcagtga
    18181 taaaaatttg ccttcgtttt ctatttccta tgccgggaat atggttggcg tggcgttaac
    18241 aaccgaaggt gaatgtggcc tcgatatgga actacagcgt gcgacgcgcg ggtttcatag
    18301 cccacacgcg cccgataacc acaccttttc cagcaatgaa tcgctatgga tcagtaaaca
    18361 aaacgatcct aacgaagcgc gggcgcagct catcacgctg cgccgaagcg tgctaaaact
    18421 aaccggtgat gttttgaatg acgatccgcg cgatctgcag ctgcagccca ttgccggacg
    18481 cctgaaatgt gctcatgtaa atcatgtaga agcgttatgc gacgcggagg acgtgctagt
    18541 gtggtccgtg gcggtcacgc ccgcgattga aaagctcagt gtctgggagt tagatggcaa
    18601 acacagctgg aaaagcctgc cggatattca cagccgcgcc aacaatccta ccagccggat
    18661 gatgcgtttt gcccagctct ctaccgtgaa gtctttttcg ccaaattgat agacaaccac
    18721 aggagtcatt atgtctgaaa aattgcaggt ggttacgtta ctggggagcc tgcgcaaagg
    18781 ctcatttaat ggcatggttg cacgtaccct gccgaaaatt gctccggcga gcatggaagt
    18841 caatgcgtta ccttccattg ccgacattcc cctgtatgac gctgacgtac agcaggaaga
    18901 aggttttcca gcaacggttg aagctctggc ggaacagatc cgtcaggctg acggtgtggt
    18961 gatcgtcacg ccggaatata actactcggt accgggtggg ctgaaaaatg ccatcgactg
    19021 gctttcccgc ctgccggatc aaccgctggc gggtaaaccg gtgttgattc agaccagctc
    19081 aatgggcgtg attggcggcg cgcgctgtca gtatcacctg cgccagattc tggttttcct
    19141 cgatgcaatg gtgatgaaca agccggaatt tatgggcggc gtgattcaga acaaagttga
    19201 tccgcaaacc ggagaagtga ttgatcaggg tacgctggac cacctgaccg ggcaattgac
    19261 cgcatttggt gagtttattc agcgagttaa gatctaaata aaaaacccgc cagtaatcat
    19321 gcatggcggg tttttaacgc gctatcgatt ttagtgagcg tcgataaaga caatcttcag
    19381 gataaacagc agcgcaacga tgattacgca cgggcttaga tcacgcagac gtccggtacc
    19441 gattttcatc acgcagtagg agataaagcc cagcgcgata ccttcggtaa tcgagaagct
    19501 gaacggcatc atcacggcgg taataaacgc cggaacagat tcagtaagat cctgccagtt
    19561 cacgcgtgcc agactggaag tcatcagcac gccaacgtaa atcagcgcac cagctgcagc
    19621 gtagcctggc accatccccg ccagcggcga cagaaagata accagcagga acagcagacc
    19681 aacaaccact gccgtcagac cggtacgacc gccaaccgat acgccggaag aggactcaat
    19741 ataagccgta acggaagaag taccgataaa cgaaccggtc acggaagaga tactgtcgac
    19801 atacagcgcc tgcttcatgc gcgggaattt acccttctca tccgccaggc ctgctttatc
    19861 ggtcacgcca atcagcgtac cggaggagtc aaacaggttg accagcatga aagagaaaat
    19921 cacccctgcc agcccgaggt taaacgactc ggctaaatct acatggccca cgacagtcat
    19981 tacgctcggc ggcgcagaaa cgatgccatt gtagtggaca tcacccagca tccagcccag
    20041 cagcgttgtc accacgatgg aaaccagcac cgccgcgtga atgttgcgcg aggccagaat
    20101 agcgatgatg aagaagccga ggatccccag cagcacgctg tgagaagtca ggttaccgat
    20161 gctcaccagc gtttccgggt tagcgacaat cacacctgca ttcttcagtc ccatcatacc
    20221 aatgaacaga ccgataccgc tggtgatgcc cacacgcaga cttaccggaa tgttggcaat
    20281 catccagtag cgaacgcgga aaatcgtcag taacagcaga ccaatcgccc cccagaagat
    20341 tgcgcccatc ccgacttgcc acggcaagcc catcgcctgc acaaccacaa aagcgaagaa
    20401 cgcattcagg cccatagcgg gtgccagtgc aactggcagg ttagcaaaca gtcccatcat
    20461 aatactgccg aatgcagcaa tcagacaggt agtaacgaag acggcgctgg tatccatgcc
    20521 agcaacgcca agaatttgcg ggttaacaaa aacgatgtaa accatcgtca ggaaggtggt
    20581 aaaaccggcg atcacttcgg tccgtgccgt cgtgccatgt tcgcgcagtt taaacacgcg
    20641 ttccagcatc ccctggccag aagcctgggt tgtgtgttgt tgactcatta tctatttccg
    20701 aacaaggagg gaaaatccgt cgctatcgta taccaaaatg agacaatatg gacggctgaa
    20761 gataactttt tttcattgta tttgcttata cggcaacgat tgcgtcatgc gaattggcat
    20821 gacgaaaacg ttaaactaac gataaaggaa agacatgtcc cagatagaag cggtattttt
    20881 cgactgcgac ggtacgctgg tcgatagcga ggtcatttgc tctcgcgcat atgtaacgat
    20941 gtttcaggaa tttggtatta cgctcgatcc tgaagaggta ttcaaacgtt tcaagggtgt
    21001 aaaactgtac gaaattatcg atattgtttc ccttgaacat ggtgttacgt tagcgaaaac
    21061 agaagctgaa cacgtttacc gtgcagaagt cgctcggctg ttcgattcag aactggaagc
    21121 catcgaaggg gctggagcgc tcctgtcagc gatcactgcg ccaatgtgtg tggtatctaa
    21181 cggcccaaat aacaaaatgc agcattctat gggcaagctg aatatgttgc actacttccc
    21241 ggataaactg ttcagcggct acgatattca gcgctggaag ccagacccgg cgttaatgtt
    21301 ccatgcggca aaagcgatga atgtaaatgt agaaaactgc attctggttg atgactcagt
    21361 tgccggtgcg caatctggta tcgacgcagg tatggaagtg ttctacttct gcgccgaccc
    21421 gcacaataag ccgatcgttc acccgaaagt caccaccttt acccatcttt cgcagttacc
    21481 ggaactgtgg aaagcgcgtg gttgggatat tacggcatag ttcttcacac tcccttcact
    21541 taccccgctt aaattggcgc tcaaaggtaa gttaagggtg tttgatatgt ctgtttcacg
    21601 tcgggtaata catcacggac tttattttgc ggttttagga ccgttaattg gtgttctgtt
    21661 tcttgtcctc tacatattct tcgcaaaaga accgctggtt cttttggtaa taatacaggt
    21721 acttccattg tttctcttat tgtcgattac tacgggagct attcctgcat tgttaaccgg
    21781 cgtgatggtc gcctgtctgc cggaaaagat cgggtcacag aaaaattatc gttgtctggc
    21841 tggtggcata ggtggcgtcg ttatcaccga gatctattgt gcagttattg tacatattaa
    21901 gggcatggct tcctcggagt tgtttgaaaa cattctttct ggtgacagtc tcgttgtccg
    21961 catcattcct gcattgctgg caggtgtggt gatgagcaga atcattaccc gtctgcccgg
    22021 attggatatt tcatgtcctg aaacagactc tttaagttaa gcgggatact ttatctttgg
    22081 gctactcaaa agcagacagg atgttactat gactcaaaat atcaggccgt taccccaatt
    22141 caaatatcat cccaagccac tggaaacagg cgcatttgaa caggataaaa ccgtagagtg
    22201 cgattgctgt gaacaacaga cgtcagttta ttactcgggt cccttttatt gcgttgatga
    22261 agttgaacat ctctgtccgt gatgtattgc ggacggttct gctgctgaaa agtttgcagg
    22321 tagttttcag gatgatgcca gcatagaagg tgttgaattt gagtatgatg aagaggacga
    22381 atttgccggt attaagaaca catatcctga tgaaatgctg aaagagttgg ttgaacgcac
    22441 gccaggttat catggatggc aacaggaatt ctggcttgcg cattgtggcg atttctgtgc
    22501 ttttatcggc tatgtgggct ggaatgatat aaaagatcgc ctcgatgaat ttgccaacct
    22561 tgaagaagat tgtgagaatt tcggtattag aaattctgat ctagctaaat gcctgcaaaa
    22621 gggtggtgat tgtcagggtt atctcttccg ctgtctccac tgcggcaagc tgagactgtg
    22681 gggtgatttt tcgtagttat ttaaataatg agaacaggcc ggagcgtaat tcatacatcc
    22741 ggccttattt cttcagctta accgagcgct aattctgcgg cagcggcttt atcagcgatt
    22801 accatcagcg aaggatgcag ctgcaacacg gaagctggaa catcttcggt aaccggacct
    22861 tgcagcacat ttttcagtgc ctgcgcttta cttgcgccac tgacaattat cagcaagttt
    22921 ttcgccgcca tgatgctttt cggccccatc gtcacgtagc tgtcaggcac taacgaaaag
    22981 tcgccgccca actcaccatg cgccacaata tcaaccatct ctccctggat cgggaactcc
    23041 acggtttgct catggaaatg ggtcgtattc ggcaaattgc cacaaaaatg accatctgca
    23101 cctaatccca gcaccaccaa atccaggccg ccttcacgcg ccagtttctg atcatgctcg
    23161 cggtagttat caatagtgag cttctggata ttctcttctt tgatccccgc aggggtgaaa
    23221 aacagattac gcagattggt aatcgttacg ccttctccct ctttgccgcg aaagggaatt
    23281 tcatcgaaat tatagaaata gcagttatcg taccagggct tacctttaac cagggtggtg
    23341 aggtattcat acatgccttt gggcgtgcta ccggcggtaa ttgccaggtt aacacgacgc
    23401 gtcttcgaca tataaccaag cagatggtgt gccgcgacac ggctcatttc ctggtaatct
    23461 tcggtaatga ttaatttcat cttaatgcct tattttattt aaataatttc tgtacgaagg
    23521 ctgcataggc aggccgccag acatccattt catggttcag gccgggatat tcctggtaat
    23581 caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc ttgccggtta
    23641 cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta atagctgccg
    23701 gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta acaccactga
    23761 atgtggccag ccagccaaag ctttccagat gattcattcc ggaaaccagc gcctggtacc
    23821 cgccttgtga aagccctgcc agcgcgcgac catcggcatc tttacggaca ttaaaacgct
    23881 tgctaatcag cgggataata tcgttcatca gttcgcgatc tgccgcttta gcattcagcg
    23941 gataaaagac tttacgtctt tcctgaggca cgaaatcttc gggaataatg cccttcgcat
    24001 cggtttcggt atccgggatc accaccagca tcggtttaat tttcccttca gccagcaggt
    24061 tatccatgat ttgcgggata cgcccctgat cgatagcgga acgtccggta tcaccaaaac
    24121 cgtgatagaa atagagcact ggcaaaggct cgcccatgcc ggtgtatcct ggcggggtcc
    24181 agacatacat ctgacgttca gattgcaatg cgttggagtg gtaagttatg gcgatcaaat
    24241 caccatgcgc aacagaacgc gtatccagat aactgcctgg caccagaatc atactggagt
    24301 taacctggcg ctgaggctta gtcattgcgg tgcctgtatc aatgctgcgt acaccatcaa
    24361 cattgaaaaa atactcgtac aggttgcctt tcaggatagg tgtgcgccac gaccagactc
    24421 ctgcttcgtc tttggtcatc gggtgaatat tgtccggaac cggaacaccc accactacag
    24481 agacattttt tgccccaggg gcaaagtagc gaaaggtcac gctgttatcg gcattgacct
    24541 gagtcacata ctgtttcacc gggatagtgg gtgccgggct ggctggcata tccgctgcta
    24601 tggcccactg agcagaaata ccgctggcaa tagccagcgt taaagcagca atttttatat
    24661 tcatcgtaat attcctttca ttaagttcca ttaccaccag atttcagcct gggcaccgac
    24721 agcaaactgg tcgtctttat tgtcttcgaa ggtgaagcca tccagttcgt tttccagggc
    24781 tttgatatac gtggcgtaga aacgaatttc cggacgcgag gtcaacatac tggtattgac
    24841 tttaaaggta tggaagagcg tggttttata accagactca ttaaatttat tactattcgc
    24901 atctttgttt tgctgggtga aatagcccag ttcaacacct gtctggttat attggtccca
    24961 aatataggcc gggcgaacaa ccgcacgaat agattcgaaa tcagagtgtg cgcctgtttc
    25021 gtagctataa atatcgttac cgaaggagta aacaatcgcg ttagccacaa taaaatgatc
    25081 accgatatag gcttcgccct gcgaggtcag acgaactgct gttccgccgg tgtgatcacc
    25141 ataataacga ccattaaagg tggtaaatgg acttgcgcca gcataacggc caaagttact
    25201 ggcgatagag ttattcgcaa ccaggaagga gaattcgttg aagccacctt tatcaaattt
    25261 ctgcgttaaa gatgtgccga acatccaggt atctttccag tcataatacc cgttattatc
    25321 ctgattatct ttttccgatg cgctttcgtt tgccgtgaca taacgaccac tcaccattaa
    25381 ggtcgcttta tcccataacg ggatatcttt atagcgtaaa tcaatagtat tggtattaat
    25441 ctgctgtttg ttttgcaggc tgcgatcgta atcatcgaca tcttcgcgaa ccagcgcgat
    25501 atcaatttta cccggaccga ctttccagtt ttccagacct acacccgctg cggcatcagt
    25561 acgctgcgtt ttccagtcca gcatctggat ttcaattttc ggcgcaccgt gtttacccac
    25621 ccagaaatca gcctctggcg caaagggcag gaaacctttg gtggtaacgt acatatcgga
    25681 gaactgcata tagttctcgc caccggcgtt atcgccaaac cagccggtag agtactgctg
    25741 accaacgtta ccatccatca tcacaacggc atcaacccgt ttgccgtttt cgttgtagac
    25801 acgttgtttt aactgcaaat caaaccagcc ggagtattcg ttaccaaagc ggcccagaga
    25861 accaatcgcc catgatttag gtgaaccatg agaggcggtc ccccaaccag aacggtaata
    25921 gccgttgtaa ctaaagccaa tttcatcttt cacaaattta ctgaaatctt tcagagtcat
    25981 ggaagaataa atcgagcctg tctcactggc atttttctct tcatttttga ccagtacagc
    26041 atcaggtttt gcggcactac tggtcgggaa cggattggcg gcataccctt gatcattcgt
    26101 acttacgcta cgattcaccg tcgctggcgt atactttttc ttctcttcat ctttatactt
    26161 tttgagttcg ctttgcgttt ctcttaacgc cttttctaat aactcaaggc gttgttccac
    26221 cgttaatgat tcagccaatg attgtgcaga aaaggctaac ggtgccatta ataagatggc
    26281 agaggtaata agatttcgtc taaacataat atccctttat ggtgcaaaga aagaattaac
    26341 gcatcgcatc cagactgttc tgaatgcgac gataattaag gtgctttaat ggttattttt
    26401 tttaatgaca gcccccgcgt cttgatcacc tctgcatacc atccgaagct tttcttgcgt
    26461 gttcttgtga ggcttccttc gccattatca tcacgatcca cataaataaa gccgtagcgc
    26521 ttagacattt gtgaatgaga tgcactgact aaatcaattg gcccccaact ggtgtacccc
    26581 ataatatcca caccatcggc aatcgcttca tttacctgta ccaggtgatc gtttaaatag
    26641 gcaattcgat aatcgtcctg tatcgaacca tccgcttcaa cgctgtcttt tgcgcctaat
    26701 ccgttctcga caataaataa cggtttttga taacgatccc aaagcgtatt taacagaacc
    26761 cgtaatccaa ccggatcaat ttgccacccc cactctgaac ttttcagatg cggattgggg
    26821 atcatattca gtatgttgcc ctgcgcattt ttattaatgc tttcgtcgtg ggaaacacaa
    26881 ccagtcatgt aataactaaa agagatgaaa tcgacggtat gttttaaatc ttctgcgtca
    26941 ctttcagtca tctcaatggt gatattgtgg tcgcggaaga aacgctgcat atagccggga
    27001 tactggccac gcgcctgaac atcaccaaag aacatccagc gccggttctc ttccatggcc
    27061 tgcaacatat cctgtggctg gcaggtgagg gggtaaacca gcccaccgag aagcatattg
    27121 ccgattttcg cttcggggag caggctatga caggctttaa ctgcccgcgc actggcaacc
    27181 agttgatggt ggatagcctg ataaacttcc gcctcgccac tctcttctgc cagccccacg
    27241 cccgtgaatg gcgcgtgtaa cgacatgttg atttcattaa acgtcagcca taacaccact
    27301 ttatgttggt agcgagtaaa gaccgtgcgg gcgtaatgct cgaagtgatc gatgaccgct
    27361 cgattagccc aaccgccgta gtttttcacc agcccatatg gcatttcgta atgggataac
    27421 gttaccagcg gcttgatccc cgcctgcgcc atttcatcaa acagccgatc gtaaaacgct
    27481 aaccccgctt cattcggttc gacttcgtcg ccctgaggga aaattcgcgc ccaggcaatg
    27541 gaaatacgca gacaggtgaa gcccatctcg gcaaataacg cgatatcttc cgggtaacgg
    27601 tgataaaaat cgatggcgac atctttgata ttctctttcc ccaggatgcg cggttccatt
    27661 tttcccatta cgccatgagg ctgtaaatct gaggtcgaga tccctttgcc atcttcctgc
    27721 caggcacctt ccacctgatt ggcagctgtt gcgccacccc aaagaaatgt ttctggaaat
    27781 gctttcataa ttaactcctt ttatcgttag cgaatgatgg ataacagcgg ttcacctgcg
    27841 cttatctgcg ccgtgccgtg gggtaatacg tccgtaaaat catcgctatt actgattaat
    27901 accggcgtcg tcagatcaaa tccggcctcg cgaatagcag ggatatcaaa agaaatcagc
    27961 cgatcgcctg tattgacctt gtcacccacg ttgacgtgag cggaaaagaa tttgccgtcc
    28021 agttttacgg tgtcgatacc gacatgaatc aggatctcca caccatcatc tgactcaatg
    28081 ccaatggcgt gtaatgtggc gaacaacgaa gcaattcgac ccgcaaccgg agaacgcact
    28141 tcaccaaccg agggcagaat ggcaatacct ttacccaaca ggccactggc aaacgtggta
    28201 tcagcgacgt gaatgagcgg cacaatctct cccgtcatcg gtgaacagat accgccctgc
    28261 tcaggtggtg taataacctc tggtgttttc tcttgcgggg caccctgcgc tggctgacgt
    28321 ttagcggtga tgaaatgaag catcaccgta ccgacaaatg cgcaaccgat ggcaatgaca
    28381 ccgccaataa cgctggccca gacggtgaaa tcaattcccg ttgacgggat ggtttgcatg
    28441 aaggtgaaaa tacttggcaa accaaaggag tagactttcg tttgcgcgta gccaatgatg
    28501 gtggccccca aagccccact gatacaggcg ataacaaagg ggtacttacg cggcaggttg
    28561 acgccatata ccgctggttc ggtgatacca aacagactcg tcaacgccgc tgatcccgcc
    28621 accacttttt tctgcgcatc gcgttcgcag aggaagacgc cgagcgccgc cccgacctgc
    28681 gccataatgg cgggcattaa cagcgggatc atggtgtcgt agcccagcac ggtgaagtta
    28741 ttgatacaca gcggcaccag gccccagtgc agtccgaaca tgacgaagat ttgccagaag
    28801 ccgcccatta ccgcgcccgc aaatgcagga accgcctgat aaagccagag ataaccggcg
    28861 gcaatcagtt cgcttatcca ggttgatagc ggccccacca gcagaaaggt gacgggtgtg
    28921 ataaccatca gacatagcaa tggtgtgaag aaatttttga ttgccgacgg taaccacgca
    28981 ttaagtcggc gttccagaat gctgcacaac caggcagaaa aaataatggg aataaccgat
    29041 gacgagtaat tcaacaatgt gaccggaata cccaggaaat ccagccccag cgcatccgct
    29101 ttttgcccgt tctcgaaagc agtcagaatt aatggatgca ctaacgctcc accaatcacc
    29161 atggcagtaa atggattacc gccgaagcgt ttccccgcgg tgtatcccag gattatcggg
    29221 aagaaccaaa acaaggcatc actggcgctg aataaaatta aataagtacc actttgttcg
    29281 gtcgtccact gaaaagtgag cgccagagcc agcatacctt tcaagatccc ggttgccgcc
    29341 atcaaaccga tcagaggcgt aaaaatacct gaaataacat aaacaaagcg gtttagcaga
    29401 ttacctttat catcattttc cggtgcctgt tgcgcttttt cgtcaaggcc tgccacactg
    29461 ttaaccgcca ggaagacatc ggccacatgg ttacctatga ccacctgaaa ctggccaccg
    29521 ctttccacca ccataataat accgggggtc tttttcagta cctctgcttg cgctttgctt
    29581 tcatccttta atttaaaacg taatcgcgtt gcgcaatgca tcagactcac aatgttatct
    29641 gcgcccccga ctcctgcgac tatttttctg gctaactccg tcataacttg ccctctaccg
    29701 ctttgcggca aaactccaaa aaaaaacctg aaaaaaacgg cctgacgtga atcaagcaat
    29761 ttttttcagg ttttgcccgc ttagtgcggt aacaatcctt tactcagtaa taatatttca
    29821 gtgttctttg cgcacgcgct ctatatttat ggctaaaaac ataatctctg cgggtgaaat
    29881 tttacgttga tactgcaaac caataaaaat ggcgatccgt tccgcacatt gccatgcttg
    29941 cgggtaattt tgttttactg cttgttgtaa tgattcatca ctatcgttaa ttgaagcatg
    30001 ttcaagaata cgccaggata aaaacttcag atgtgtaacc agtcgctgat aactcaagct
    30061 ttcttcctgg taattaaggc tgaactgaaa ttttattaat tgcagcattt cgcgcattaa
    30121 ctgcgtgaca cctgcaacat cctccatatt tccgctcatt tgggcactga ccagatgcat
    30181 ggcaataaag cccacttcat ctttcggtaa ctgcacgccc aaccgtttat caataatggt
    30241 taacgcttct tcccctagct ggaactcttt cgggtaaagc cgctggatat cccacagcaa
    30301 cgggttgggc agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt
    30361 tagcgagata taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataat
    30421 acgatcacag gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc
    30481 gttcagttca tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc
    30541 gccagcgcgt ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc
    30601 atcaataacc accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag
    30661 gacctttttt ataaacaaaa aaacccgact tcaccagtat tctctggtta tgtcaggttt
    30721 tgcctgcgaa tgcagtaaca atccagtcat ttattaatgg tttttataac gaacatccag
    30781 gttcggaaat taatttaatt gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat
    30841 gaccatgctc gcagttatta actttgtgta attttaggaa tttataaagt tatatataac
    30901 aaatcccaat aattaagtta ttgggatttg tctggtgaat tatttgtcgc tatctttccc
    30961 cgccagcagt ttatccagct catcgccacc gacgtgacgg aaatcctgcc ccttcacgta
    31021 gtagaagata aactcacaaa tattctggca gcggtcgcca atacgttcga tagaacgcgc
    31081 gcagaacagc gcagtaagta cgctcggaat ggtacgcgaa tcttccatca tgtaggtcat
    31141 cagttgacga acaatacctt cgtattcctg atcgactttt ttatcttcac gataaatacg
    31201 taccgcttcg tcaatgtcca tccgcgcgaa cgcgtccagc acgtcgtgca gcatctggat
    31261 ggtatgacgg cccagcgact ccagacttac cagcaacggc tgatgctgct gggagaattt
    31321 ctccagcgca gtacggcaga ttttgtccgc cacgtcgcca atacgctcca gctcggcaat
    31381 ggttttactg atcaccataa ccagtcgcag gtcgctcgcc gtcggctgac gtttggcgat
    31441 aatgcgcacg caggcttcat cgatcgccac ttccatcatg ttgacgttct tgtcgccttc
    31501 gatgacgcgc ttcgccagat cgctgtcctg gttatgcatc gcggtgattg catcagaaag
    31561 ctgctgctcc accatgccgc ccatggtcat cacctgcgtg cggatacttt ccagttcggc
    31621 gttgaactgg ccggaaatat gtttattaag attgagactg tccataacgc actcctgaat
    31681 caaccgtaac gaccggtgat gtagtcttct gtttgtttct tcgctggctt ggtgaacaga
    31741 tcgtccgtgt tgctgaactc aatcaattcg cccaggtaca taaacgccgt gtggtcggaa
    31801 caacgcgcag cctgctgcat gttgtgggtg acgatcacca cggtgtaatc ctgcttcagt
    31861 tcggtgatca gctcttcaat acgcccggta gagatagggt cgagcgccga acacggttcg
    31921 tcgagcagca gcacttccgg gcgaatggcg ataccacgcg caatacacag acgctgttgc
    31981 tgaccaccag agagagagta accgctctgg tgcaatttat ctttggtttc gttccacaat
    32041 gcggctttgg tcaatgccca ctgcacgcgc tcgtccatat cggcacggga gagcttctca
    32101 aacagacgaa cgccaaaagc gatgttgtcg tagatggaca tcgggaacgg cgtcggtttc
    32161 tggaacacca tgcccacttt cgcacgcagc agggcgatat cctgagagtt ggtcaggatg
    32221 ttgtcgccat caagcagaat ttcaccttcc gcacgctgct ccgggtacag ttcaaacatt
    32281 ttgttgaagg tacgcagcag cgtcgattta ccgcagccgg acggcccgat aaacgccgta
    32341 acctggtttt tggcgatatc caggttgatg tttttcaggg catggaattt gccgtagtag
    32401 aagttcaaat tacgaacctg aattttactc ggggcagttt caaccatact cattgcaatc
    32461 ttttcctcat cgtgcctgat gcacttcgct aatcaggctt accgtaggcc ggatcaggcg
    32521 ttcacgccgc atccggcaaa gtgttaaccg tgtacagagt caaaaaggcg ctcggtcggt
    32581 cccctcgccc ctctggggag agggttaggg tgaggggaaa accgtgtcag caatatcaac
    32641 cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagt acgcacaggg
    32701 taatgatcaa taccccggcc caggccaatt gctgccattc cgcaaacggg ctcatcgcaa
    32761 acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg ctccagaact
    32821 ggttggagag cgcggtaaac agcagcggcg cggtttcacc agcaatacgg gcaatcgcca
    32881 gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgca gagatcatct
    32941 tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc accagtttca
    33001 gcatgttctc ggtggtgcgg ataacaatcg gcacctgcaa caacgccagg gcaatcacgc
    33061 ccgcccagcc ggagaagtgc tccatctgcg ccaccacaat ggtgtaaaca aacagaccaa
    33121 ccacaatcga cggcgcagag agcagaatgt cgttaatgaa gcgaatcact tctgccagcc
    33181 aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc ggcgtaccga
    33241 ataccgtggc ccacaaaatt aacaggccgc tacccgccag ggcgttcgcc agaccaccac
    33301 cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata ccatcgatac
    33361 cgcgagtgat ggtggacatt aaaatccaga tcagccagaa cagaccgaag gccatcgtcg
    33421 ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc attttgcggc
    33481 gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc gtgccccctc
    33541 attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga aggtaatcac
    33601 aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt ccgcttccgc
    33661 aaattcgttc gccagcgcag aggtgatact gttgcccggc atatacagcg aggcgctgtc
    33721 gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga gcgcgcggcc
    33781 cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa cgatacgcca
    33841 gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca tcaccggggt
    33901 ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga tggcgaggat
    33961 cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg cgccaacaat
    34021 cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga acagcggcgc
    34081 aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca gcagctcaat
    34141 ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga acagggcgat
    34201 accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca acgtaccgta
    34261 gatcggcacc agcgccccgt agatatcgtt cggcgcatcc cactctttgg tccacaggaa
    34321 agccagacca aatttctgaa tgctcggcca tgaggagatg atcagagaga caataatgcc
    34381 acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga aaattatgtc
    34441 gcccttttta cccggtgggt taaaagcagg cttggttgca gccataagtt actcttcagt
    34501 taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac cgcacccggc
    34561 ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt cttccacgca
    34621 gcgcgaacct gttccactac gctatccggc aggctggcgt aatccaggtc atttgcctgt
    34681 ttagccccgg ttttatacgc ccagtcgaag aacttcagga cttctacacc ctgctctggt
    34741 ttcttctgat ctttgtggac caaaatgaac gtggtagagg taataggcca tgcatcttcg
    34801 cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc ttttgctgca
    34861 ttagcgaagt tttcttcagt cggcaaaaca ggtttaccat cagcggagat cagtttggtg
    34921 tacgccaggt tgttttgctt cgcataagca tattcaacgt aaccaattgc acccggcaga
    34981 cgctgaacga acgcagcgat accgtcgtta cctttaccac ccagaccgat cggccatttt
    35041 acggtagagc cagtaccaac gttgtttttc cactcttcat tcactttcgc caggtagctg
    35101 gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat gttctgtgaa
    35161 ggcagtttca gatccggatt cagtttggcg atggcttcat catcccactt cttgattttg
    35221 ccgaggtaga tgtcgccgag ggttttacca tccagcacca gctcgccaga cttcaaccct
    35281 ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa cagaccttcc
    35341 tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac ggtattagcg
    35401 ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt attaccggtt
    35461 tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt tgcacctgca
    35521 cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt cgcggcgaca
    35581 acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat tcataaagca
    35641 ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata tgtaaggaat
    35701 atgacagttt tatgacagag agataaagtc ttcagtctga tttaaataag cgttgatatt
    35761 cagtcaatta caaacattaa taacgaagag atgacagaaa aattttcatt ctgtgacaga
    35821 gaaaaagtag ccgaagatga cggtttgtca catggagttg gcaggatgtt tgattaaaaa
    35881 cataacagga agaaaaatgc cccgcttacg cagggcatcc atttattact caaccgtaac
    35941 cgattttgcc aggttacgcg gctggtcaac gtcggtacct ttgatcagcg cgacgtgata
    36001 agccagtagc tgcagcggaa cggtgtagaa gattggtgca atcacctctt ccacatgcgg
    36061 catctcgatg atgtgcatgt tatcgctact tacaaaaccc gcatcctgat cggcgaagac
    36121 atacaactga ccgccacgcg cgcgaacttc ttcaatgttg gattttagtt tttccagcaa
    36181 ttcgttgttc ggtgcaacga cgataaccgg catatcggca tcaatcagcg ccagcggacc
    36241 gtgtttcagt tcacctgcag cgtaggcttc agcgtgaatg taagagatct ctttcagctt
    36301 caatgcgcct tccagcgcga ttgggtactg atcgccacgg cccaggaaca gcgcgtgatg
    36361 tttgtcagag aaatcttctg ccagagcttc aatgcgtttg tcctgagaca gcatctgctc
    36421 aatacggctc ggcagcgcct gcagaccatg cacaatgtca tgttcaatgg aggcatccag
    36481 acctttcagg cgagacagct tcgccaccag catcaacagc acagttaact gagtggtgaa
    36541 tgctttagtg gatgccacgc cgatttctgt accggcgttg gtcatcagcg ccagatcgga
    36601 ttcgcgcacc agagaagaac ccggaacgtt acagattgcc agcgaaccaa ggtaacccag
    36661 ctctttcgac agacgcaggc ccgccagggt atccgcggtt tcgccagact gtgacaaggt
    36721 gatcatcagg ctgttacgac gcacggcaga tttacgatag cggaattcag aggcgatttc
    36781 gacgtcgcac ggaatacctg ccagcgattc aaaccagtag cgggaaacca taccggagtt
    36841 ataagaagta ccacaggcga ggatctgaat atgctcaacc ttcgacagca gttcgtcggc
    36901 gttcggtccc agctcgctta aatcaacctg accgtggctg atgcgcccgg taagggtgtt
    36961 ttttatcgcg ttcggctgtt cgtagatctc tttctgcatg tagtgacggt aaataccttt
    37021 atcgcccgcg tcatattgca gattggattc gatatcctga cgttttactt ccgcgccagt
    37081 tttatcgaag atgtttaccg aacggcgagt aatttccgca atatcgccct cttcaaggaa
    37141 gataaagcga cgggtcaccg gcaacagcgc cagctggtca gaagcgataa agttttcgcc
    37201 catacccagg ccaatcacca gcggactacc agaacgtgcc gccagcaggg tatccgggtg
    37261 acgggagtcc atgatcactg taccgtacgc accgcgcagc tgcgggatag cacgcagaac
    37321 agcctcacgc agagtcccgc cttgtttcag ctcccagttc accagatggg caattacttc
    37381 ggtgtctgtt tcagaaacga aggtatagcc acgcgctttt aactcttcac gcagcggttc
    37441 atggttttcg atgatgccgt tatgcaccac cacaatgtgt tcagaaacat gcggatgcgc
    37501 gttagcttct gaaggttcac cgtgggtcgc ccagcgagtg tgagcaatac cggtgccgcc
    37561 atgcagagga tgttcttccg ctgcctgagc cagcatctgg actttaccga ggcgacgcag
    37621 gcgggtcata tgaccttccg catcaacaac ggccagaccg gcagagtcat atccgcggta
    37681 ttccagacga cgtaaacctt caagaaggat ttctgctaca tcacgttgcg cgatcgcgcc
    37741 aacaattcca cacatagttt ttgattccga tttatatcgt tgtcggtcaa cctgtatgcc
    37801 cgttttttcg ggcgccccga gccttgtaga gagtggggtt atttttatag ttactgcttg
    37861 tgggagggag attatgttat ctcctcatcc cagatgaccg gattacccgg ccagaattac
    37921 tttttcttta ccggacgacg ccagccttct ttctgagtct gcggcacacg gctgatagct
    37981 aatgcatttt cgccgacatt acgcgtcaca gttgtacccg cagcaatggt cgcgcctttg
    38041 cctactgtta ccggggccac cagctgagtg tcggaaccga caaacacatc gtcgccgata
    38101 atggtcttaa atttattcgc accatcgtag ttgcaggtaa ttgttcccgc gccgatgtta
    38161 acgttatcgc caatttccgc atcgcccagg taagtcagat gcccggcttt cgagccttta
    38221 cccagacgcg cttttttcat ctcaacgaag ttaccaacgt gtgcaccttc cagcaactca
    38281 gcaccaggac gcagacgggc aaacgggcca atagtacagg ccgctgccag attcgcgtct
    38341 tccacaacgg tatacggact gatttcgcaa tcatcgccaa tcacgctgtt tttaatcacg
    38401 caaccggtgc cgattttcac gcgatgaccg agagtcacgt tgccctcgat gataacgtta
    38461 gtatcaattt caacatcgcg cccgtgagta agcgtaccgc gcagatcaaa acgcgccgga
    38521 tcgcgcagca taacgcctgc taacagcagt ttttcagcct gttcggactg gtaaacgcgc
    38581 tccagacggg agagttgcag gcggttattc acaccttcta cttcgcttaa acgttgcgga
    38641 tgaacggcga cgatttcacg cccttcctga tacgccagcg caataatgtc ggtgatgtag
    38701 tattcgccct gagcattatt gttggtcagc ttcgccagcc agcgtttcat atctgcgccg
    38761 ttagcgatca gaatgccggt gttgatctcc tgaatctgac gctgctcgtc ggtggcatct
    38821 ttgtgctcaa caatgccggt aactttgcca ttttcacggg tgatacgtcc ataaccggtc
    38881 ggatcatcca gtttcaccgt cagcagacca atgccaccct gcggtttagc atcacgcagg
    38941 cgctggagtg tttcgacaga gatcagcggc acgtcgccgt agagcattaa aatgtcttca
    39001 tcatcggcaa agaaaggtgc ggcctgctgc attgcatgac ccgtacccag ctgctctgcc
    39061 tgaagcaccc agttaaggtt gtcgtctttc agcgcctgtt taagcagatc gccgccgtga
    39121 ccgtacacca ggtgaacgtg cgctgcgcct aattcattcg cagcatcaat gacatgctga
    39181 accatcgctt tcccggcaag ggtatgcagc actttcggaa gatcggaata catgcgcgtg
    39241 cctttgcctg cggcaaggat cactacgctc atagcattat tcaacatacg cgtcctgact
    39301 gtaatttgag aacgaattta aaccgcttca ccttgaaaaa actacatttt tttcatcgtg
    39361 aaatggacag aggataaatt gttcaataac ggattatccc cgctgacgaa agtgccattt
    39421 tcgaccatcg tcacgccttt tgtctgcaga aaattaaggc agtaccttca tttaagcgtc
    39481 aggtggatgt ttttgctctt atttcgatca atgaataaac gggaaaacag gggtgtttta
    39541 tatcagcagg atctatgtga acgctcttca ggacggatca cgcgtaaaaa aaagccagcc
    39601 tgtttccaga ctggcttttg tgcttttcaa gccggtgtta catcgctttt ttggtcaact
    39661 cgataacgcg cagctgcgcg atcgctttgg ccagttccgc agacgcctga gcgtaatcta
    39721 cgtcgccgtg agagctgcta atgtgctctt cagccttacg tttcgcttcc atggctcgcg
    39781 cttcgtcgag atcctggccg cgaattgcgg tgtcggccag aacggtcacg ttgccaggct
    39841 gcacttcaag aatgccgcca gacagataga taaactcttc gtgaccgtgc tgtttcacga
    39901 tgcgaatcat accaggctta atggcggtga gcagcggtgc gtggccaggg tagatcccca
    39961 gttcaccttc gctacccgtt acctggattt tctcgaccag accagagaac atttgttgct
    40021 ctgcgctgac gacgtccagg tggtaagtca ttgccatatc accctccgat taaggcgtta
    40081 aagttttttg gctttttcca cagcttcttc gatggaaccg accatgtaga acgcctgctc
    40141 cggcaggtga tcgtattcgc cttccatgat gcctttaaag ccacggatgg tgtctttcag
    40201 ggagacgtat ttacccggag aaccggtgaa tacttctgcc acgaagaacg gctgggacag
    40261 gaagcgctgg atcttacgag cacgcgctac caccagtttg tcttcttcag acagttcatc
    40321 catacccagg atggcgatga tgtctttcag ttcctgataa cgttgcagga tggactgaac
    40381 gccacgcgcg gtgtcgtagt gttcctgacc aaccaccagc gggtccagct gacggctggt
    40441 ggagtccagc gggtcaacgg ccgggtagat acccagagac gcgatctgac ggctcagtac
    40501 cacggttgcg tcaaggtgcg caaaggtggt tgccggagac gggtcagtca agtcatccgc
    40561 aggtacgtat actgcctgta cggaggtgat agaaccagtt ttggtggagg tgatacgttc
    40621 ctgcagaacg cccatctctt ccgccagggt cggctgataa cctaccgctg aaggcatacg
    40681 gcccagcagt gcggatactt ccgtaccggc cagggtgtaa cgatagatgt tgtcaacgaa
    40741 cagcagaacg tcacgacctt cgtcacggaa tttctcagcc atggtcagac cggtcagagc
    40801 aacgcgcaga cggtttcccg gcggctcgtt catctggcca tacaccaggg atactttgtc
    40861 gataacgttg gagtcggtca tttcgtggta gaagtcgtta ccctcacgag tacgttcacc
    40921 tacgcccgca aacacagagt aaccggagtg ctcgatcgcg atgttacgaa tgagctccat
    40981 catgtttacg gttttaccta cacccgcacc accgaacaga ccaactttac cgcccttagc
    41041 gaacggacac atcaggtcga taactttgat accggtttcc agcagttcct gagagtttga
    41101 cagctcttcg taggaaggtg ctgcgcggtg aatcgcccaa cgctcttctt caccgatctc
    41161 gcctttcatg tcgaccggtt cacccagtac gttcatgata cggcccagag tcgctttacc
    41221 taccgggact tcaatcgggt gttcgaggtc ttttacatcc agaccgcgac gcagaccgtc
    41281 ggaggaaccc attgcgatgg tacgtacgat accgccgccg agctgctgct gaacttccag
    41341 caccagacgc tcattaccat tttgcacctc aagagcatcg tacacgcgcg gtacggcatc
    41401 ctgagggaat tcgacgtcaa ctacggcgcc gattacctgg acaatctttc cagtagccat
    41461 cttaaatcct ctacgaaata acctgtttaa accgcggcgg cccccgagac gatctcggtg
    41521 agttcctgag taatgctggc ctgacgagct ttgttgtata ccaactgcag ctctttaatc
    41581 aggctgccgc cattgtcggt cgcggctttc atcgccacca tacgggcggc ctgctcgctg
    41641 gccaggtttt caaccacgcc ctgataaacc tgagattcga cataacgacg cagcagggta
    41701 tccagcaacg ccttcggatc gggttcgtac aggtaatccc aggatttatg tttcagatca
    41761 tcatcatctg atgccggtaa cggcagcagc tggctgatgg tcggaacctg agacatggtg
    41821 ttaataaatt tgttgctgac aatgtaaagc ttgtccagac ggccttcgtc gtaggcctgc
    41881 aacatcactt ttaccggacc gatcagttcg gacagggaag ggttatcccc catgccggtg
    41941 acctgggcaa caacattgcc gcccacggag ttgaagaacg acacgccttt cgagccgatc
    42001 attgcgaggt cgcattgaac gcctttgtcg gtccaggtct tcatttccgc cagcagtttt
    42061 ttgaacaggt taatgttcaa accaccgcac aaaccacggt cggtcgacac caccaggtag
    42121 cccacgcgtt taacgtcgcg gtcttccagg taagggtgct tatattccag attaccgtgt
    42181 gcaaggtgac caatcacttt gcgcatggtt tctgcataag gacggctggc cgccatgcga
    42241 tcctgcgatt tacgcatttt ggaagcggcg accatctcca tcgctttagt gatcttttgc
    42301 gtgttctgga cgcttgcgat cttactacgt atctcttttg cgccggccat gagcttctcc
    42361 tcaatgcctt gcggcctgcc ctaaggcaag ccgccagacg ttaccaggat tgggttgctt
    42421 tgaaggaatc gaggatgcct ttcagcttgc cttcgatttc gtcgttgtag ccaccggtct
    42481 ggttgatctc ttgcatcaac ggagcgtgat cacggtcgac gtaagccagc agagcggctt
    42541 cgaagctgcc aattttcgac agttcaacat ccgccaggta accacgttct gctgcgaaca
    42601 gaaccagaga ctgctgcgca acggacatcg gcgcatactg tttctgtttc agcagttcgg
    42661 tcactttctg accgtggtca agctgcttac gtgttgcatc gtcaaggtcg gatgcaaact
    42721 gagagaacgc tgccagttca cgatactgtg ccagagcggt acggatacca ccggacagtt
    42781 ttttcatgat cttggtctgt gctgcaccac caacacggga tacggaaata cccgggttaa
    42841 ccgcaggacg aataccggcg ttgaacaggt tggtttccag gaagatctga ccatcggtaa
    42901 tggagattac gttggtcgga acgaacgcag aaacgtcacc cgcctgagtt tcgataatcg
    42961 gcagtgcggt cagagaaccg gttttccctt tcacttcacc tttggtgaag gcttcaacgt
    43021 attcggcgtt aacacgtgca gcacgctcca gcagacgaga gtggaggtag aaaacgtcgc
    43081 ccgggaatgc ttcacgtcct ggcggacgac ggagcagcag ggagatctga cggtaagcaa
    43141 cagcctgttt agacaggtca tcgtaaatga tcagcgcatc ttcaccgcgg tcacggaagt
    43201 attcgcccat tgcgcaaccg gcatacggtg ccaggtattg cagtgcagcg gattcagacg
    43261 cggttgctac cacaacgatg gtgttagcca gtgcgccgtg ctcttccagt ttacgtacca
    43321 cgttagaaat ggtggacgct ttctggccga tagcgacata gatacatttg ataccggaat
    43381 cgcgctggtt gatgatggca tcgatagcca gtgcggtttt acctgtctga cggtcaccga
    43441 tgatcaattc acgctgacca cgaccgattg ggatcatgga gtcaacggct ttataaccgg
    43501 tctgtaccgg ctgatctacg gactgacgtt cgataacgcc cggagcgatt gcttctacag
    43561 cagagaagcc gtcgtgatcc agcggacctt taccgtcgat tggtgcaccc agagtgttaa
    43621 ccacacggcc cagcaggcca cggccaaccg gaacttccag gatacggcca gtacacttaa
    43681 ctttcatgcc ttcggcaagg tcagcgtacg gacccataac aaccgcacct acagagtcgc
    43741 gctcgaggtt cagtgcgata gcgtaacggt tacccggcag ggagatcatt tcaccctgca
    43801 tacaatcggc caggccgtga atgcggataa caccgtcact tacagaaaca atagtacctt
    43861 cgttgtgagc ttcactcaca acattgaact gagcaatgcg ctgcttgatc agttcgctga
    43921 tttcggtgga attcagttgc atgctccagt ccccttaaga ctgcaagacg tctgcaaggc
    43981 gctcaagacg accgcgtacg ctgccatcaa tgaccatatc acccgctcgg atgataacgc
    44041 ctgccattac agacttatcg attttgcaat tcagcttaac tttgcgtgac agacgttttt
    44101 ccatcgcagc agaaattttc gcgagctgtt gttcactcag tgcggcagcg gaaatgacgt
    44161 ctacctcagc ggtagcctca ctcacggcac gcaggtgaat aaactgctcc agaacatccg
    44221 ggagcgcgtt aagacgacca ttttcagcca taacccgaat caggttctga ccgttttcgt
    44281 ccagttgctc accacaaact gcgataaacg actcggcgag cgtttctggc gcaagcgcgc
    44341 cagagagaag ctctgccatt tgttcgtttt tggttacctc ggcgacaaac gccagcatgt
    44401 cctgccagcg ttctacactt tggtgttcga cggcaaagtc aaaagctgct ttggcgtagg
    44461 ggcgagctac cgtaataaat tcagacatca gcccctccct ccttacagtt cagcgacaag
    44521 tttatccacg atgtcgctgt tagcagcttc atccacggaa cgttcgatga tcttctcggc
    44581 gccagcaaca gccaggatag caacttgctt acgcagctct tcacgggcac gtttacgctc
    44641 ggcttcaatt tccgcctgcg cctgggccac gattttagta cgttcctgtt ctgcctcagc
    44701 tttcgcttcg tccagaatct gcgagcggcg tttgttcgcc tgctcgatga ttacctgggc
    44761 ttccgctttc gcttttttca gctggtcggt cgcgctggcc tttgcaaggt caaggtcctt
    44821 atgtgctcgt tctgcggaag caaggccgtc agcaatttct ttttgacgtt tttcgatggc
    44881 tgccattaat ggcggccata cgtacttcat gcagaacaga acgaacagga caaacgcgat
    44941 ggcctggccg aggattgttg cgttaagatt cacagcacaa tgcctctatt tagttaacgt
    45001 tctgatattg ctctttaaat aaaagcaacg cttactacgc gacagcgaac atcacgtaca
    45061 gacccagacc tacagcgatc atcgggatag catccaccag acccataacg ataaagaact
    45121 gagtacgcag cagaggaatc agatcaggtt gacgcgctgc gccttccagg aatttacccc
    45181 cgaggatgcc gataccgatc gcagcaccga ttgccgccag acccatcatc acagcggcag
    45241 ccatgtacag cagatccata ttcaggtttt ccatgacagt ctccagtttg tttcagttaa
    45301 aacgtagtag tgttggtaaa ttaatgttct tcagacgcca tcgacagata gacgatcgtc
    45361 agaaccatga agatgaaggc ttgcagcgta atgatcagga tgtggaaaat ggcccacggc
    45421 acattcagga tccactgtga ccaccacggc aacagaccag caatcagaat gaaaatcagc
    45481 tcaccggcat acatgttacc gaacagtcgc aaaccgagtg aaactggttt ggacagcagg
    45541 cttacccctt caaggattaa gttgacagga atgaacgccc agtgattgaa cggctgcagc
    45601 gtcaactctt tcgtgaagcc gccgatgcct ttcattttga tgctgtagaa cagaatcagg
    45661 ataaatacgc ccagtgccat agacagcgtt acgttcacgt ccgcagacgg aaccacacgc
    45721 agtgcaggca gacccagtac atgttcagca atgtacggca gcaggtcgat aggcagtaaa
    45781 tccatcaggt tcatcaggaa tacccagacg aagatcgtca gggccagcgg agcaatcagc
    45841 ttgcttttgc catggtacat gtctttcacg ctaccattaa caaagccgat caccagctca
    45901 atcgcggtct gaaacttacc tggcacaccg ctggtcgcct ttttggctac gctacggaat
    45961 aaaaccagga acaacagacc cagcaccacc gagaagaaca tggagtcaat attgattgtc
    46021 cagaaggtgg ctggggggtt ttgtggatcc accagcgaga atgtacgcag gtccagctga
    46081 aggttattca ggtggtgtcc tatgtaatcc tgcggcgtca tattttctga agccatgatg
    46141 ccttttaccc tttgttgtta attacagccg gtgccagtat ctgaaccacc agcaccaaaa
    46201 cccacgtaac gatcagcggc aagaataccg cctttaaaac cgccaacgcc accaccagta
    46261 acaccaacat cgccagaact ttgaaagctt cgccaaatgc gaatgtccag gccacccggc
    46321 ctttcgctgg tgtatgcgcc tggtgacgcc aggcaaatat cataaacaaa acgttaggca
    46381 gaaagactgc caggcccccg cttattgcag agacgcccca gaaggggtct ttgaggctga
    46441 acagcaatcc acttgctatc accaccagta actgaacgag cagaagcttc cgagcaacgt
    46501 ttcgactcac gagcgacaca gacatcacgt ttttcactcc tgctcccttc gaggtatgcc
    46561 gcgtgtcgta taaaactttc tttaaggctt agagtcaagc atcaaaaagc ggtcaaatta
    46621 tacggtgcgc ccccgtgatt tcaaacaata agtagccaaa aggtgaataa atgtttaaat
    46681 atttttccag tgcatacaat tgcgactttt ctgctaaccc tgttcgatca tgaaaaactg
    46741 taaataacgc gtaaacactg gtgataaagc gtgcttcaga tcacatattg cgcatgttcg
    46801 cgcacagcat atttatttac ttggcaaatg atgcctttgc aagtttatga tatttcagtc
    46861 taaaaacaga tactgtttta ataaatgaca tttacacaac aaaaaccacc cattgacatt
    46921 tttaataatg ttttaacagc caatgatggt tcttagcgcc gatttttagc agactgatat
    46981 tttcactaat gacttatttt ctgcttacca aaaaaaagcc acgttatctt gttgatgcaa
    47041 aagagtgaac gtggcgttaa atgtaaccag ttatatcagt agaaaacctg gttgttgtta
    47101 acagtctaac cggtcaattt tttatgattt ttttgataaa aattaaattt tatttgcttt
    47161 aatcaccacc agatgacgtt cgccatccag ggctggaacc tgaagtttaa ccactgattc
    47221 gacctgatat tcttcgggca acaaagcgat ttcatcttcc ggcatttgcc ctttcagcgc
    47281 gtagaaacgg ccttgctcac caggaagatg gtggcaccag ctcaccatat cgttcagaga
    47341 ggcaaaagcg cggctaatta cgccatcaaa tggcggctct gaaggaaact cttctaccct
    47401 gctctgtact ggttcaatat tctccagttt aagctcatgt tgcacctgac gaaggaaacg
    47461 cacgcgttta ccaaggctat ccaacagagt gaaatgggct tcaggacgca cgatagagag
    47521 tggaatgcct ggcagtcctg gtccggtgcc gacatcgata aaccgttcac cttgcagata
    47581 cggtgccacc acaatgctat cgagaatatg gcgtaccagc atctcattag gatcgcggac
    47641 cgaagtcagg ttgtacgctt tgttccattt atgcagcata ttcacgtagg caataagctg
    47701 gtttttctgg tgatcggtaa gcgaaatacc tgcgtctttc agcagtaagg agagtttgtt
    47761 gagcacggtg attacctgtt cttgatgcgt tgcctggtaa gcgggtgctt accaggcatt
    47821 tttaatgcgt tatgcgctac gacgcagcat accctgtttt ttcagccaca ccagcagaat
    47881 ggagatggcc gcaggcgtga cgccagaaat acgcgaagct tggccgatag aggctggttt
    47941 gtgatcgtta agtttggcga tcacttcgtt agaaagaccg gatacctggc ggtaatccag
    48001 tgtcgcgggt agcagggtgt tctcgttacg cagctgcttt tcgatctcat cttgctggcg
    48061 cgcgatataa ccttcgtatt taacctgaat ctcaacctgt tccgccgcct gttcgtctgt
    48121 caacgcaggg gcaaacggcg tcagcgtggt taatttttca taagtcattt ccggacgacg
    48181 cagcagatct tcaccactgg cttcacggga aagcggcgca gtcaggtgag cattcacttc
    48241 ggctgcagct tccgccgacg gggttaccca ggtcgatttc agacgctgac gctcacgctc
    48301 gatattctca agtttctcgt taaagcgcgc ccaacgttcg tcatccacca ggcccagttc
    48361 acgaccgatt tcagtcaaac gcagatccgc attatcttcg cgtagcatca gacgatattc
    48421 tgcgcgcgaa gtaaacatac gatacggttc tttggttcct aaagtgcaca ggtcatcaac
    48481 tagtacgccg agatacgcct gagaacgtgc cggagcccaa ccttctttgt cagcagacag
    48541 acgggcagcg ttaagaccgg ccagcaaacc ttgcgcagcg gcttcttcgt aaccggtagt
    48601 gccgttaatc tgaccagcaa agaacagccc ctggataaac ttgctctcca gcgtcggttt
    48661 caggtcgcga ggatcgaaga agtcatactc aatggcataa cccggacgca cgatcttcgc
    48721 gttttccatc ccctgcatag agcggacgat ttgcatctgc acatcgaacg gcaggctggt
    48781 ggagataccg ttcggataaa tttcattaga ggtcagtcct tccggttcaa ggaagatctg
    48841 atgctgattt ctgtcggcga agcgcatgac tttgtcttcg atcgacgggc agtagcgtgg
    48901 gccgacacct tcgatcaccc ctgcgtacat tgggctacga tcgaggttac tgcggatcac
    48961 atcatgggtt ttctcgttgg tatgagtgat ataacacggc acctgctggg gatgctggga
    49021 cgcattgccc ataaacgaga ataccggcat tgggttatcg ccatgctgtt gcgccagtac
    49081 gctaaagtcg atggttcgag catcaatacg cggtggtgtc ccggttttca gacgaccaac
    49141 gcgcagcggc agttcacgca aacggcgaga aagcggaatg gacggcggat caccagcacg
    49201 gccaccgctg taattatcca gaccgatatg aattttaccg tcgaggaacg tcccaacggt
    49261 gagcacgacg gctttggcac ggaacttcag tcccatttgg gtaacagcac cgaccacgcg
    49321 atcgttttcg acaataagat cttcaaccgc ctgctggaag atcatcaggt tcggttggtt
    49381 ctccagcgcc gtacgtaccg cctgacggta gagcacacga tccgcctgag ctcgggtagc
    49441 gcgaaccgcc ggtcctttgc ttgcgtttag tatcctaaac tggatacccg cctgatcgat
    49501 cgctttcgcc atcagaccgc cgagtgcatc cacttctttt accagatgtc ccttcccaat
    49561 accgccgatc gccgggttgc agctcatctg ccccagagtg tcgatattgt gtgtcaaaag
    49621 cagagtctgt tgacccatac gcgccgcggc catcgcggcc tcggtgcctg catgaccccc
    49681 gccaatgata atgacgtcaa aaggatccgg ataaaacatg gtgattgcct cgcataacgc
    49741 ggtatgaaaa tggattgaag cccgggccgt ggattctact caactttgtc ggcttgagaa
    49801 agacccggga tcctgggtat taaaaagaag atctatttat ttagagatct gttctattgt
    49861 gatctcttat taggatcgca ctgccctgtg gataacaagg atccggcttt taagatcaac
    49921 aacctggaaa ggatcattaa ctgtgaatga tcggtgatcc tggaccgtat aagctgggat
    49981 cagaatgagg ggttatacac aactcaaaaa ctgaacaacc gttgttcttt ggataactac
    50041 cggttgatcc aagcttcctg acagagttat ccacagtgga tcgcacgatc tgtatactta
    50101 tttgagtaaa ttaacccacg atcccagcca ttcttctgcc ggatcttccg gaatgtcgtg
    50161 atcaagaatg ttgatcttca gtgtttcgcc tgtctgtttt gcaccggaat ttttgagttc
    50221 tgcctcgagt ttatcgatag ccccacaaaa ggtgtcatat tcacgactgc caataccgat
    50281 tgcgccaaag cggactgcag aaagatcggg cttctgttcc tgcaatgctt catagaaagg
    50341 agaaaggttg tccggaatat ctccggcacc gtgggtggag ctgataacca gccagatccc
    50401 tgaggcaggt aaatcttcta acagcggacc gtgcagcgtt tcggtggtaa aacccgcctc
    50461 ttccagcttt tcagccaggt gttctgctac atattcggca ccgccgaggg tgctgccgct
    50521 gataagagtg atatctgcca taaaccgcca cctttattaa gagtggcgta ttgtacgctg
    50581 tgaacgcgtt gggatctacc tgtggaaaag tatgggatta aaaaagccga tcagggcttg
    50641 atggtacgca tgatcgggtt ttgcaggacg atcaatgtct cggtggactg aatttcatca
    50701 attgtttgga tcttgttgat aagtacatgc tggagagcgt cgatcgaacg gcacatcact
    50761 tttataaaga tgctgtagtg gccggttgtg taataggctt cagtgacttc atcaaggctt
    50821 tccagctttg ccagcgcgga agggtagtct ttggcgctct ttaatataat gccgataaag
    50881 cagcctacgt cataaccgag ctgcttcggg ctgacatcaa tacgcgcccc ggtaatgatc
    50941 cccgcctgct tcattttctc tactcgaacg tgaatcgtcc ccggactgac gccaaattgt
    51001 ttcgccagtt cggcgtaagc ggtgcgcgca ttgcccatta atgcttccag gatgccacgg
    51061 tccagattgt cgatcagata attttccata ggattttctt atgcggattg atgattcatt
    51121 ctattttagc cttctttttt aatgaatcaa aagtgagtta ggctttttat tgaatgatta
    51181 ttgcatgtgt gtcggttttt gttgcttaat cataagcaac aggacgcagg agtataaaaa
    51241 atgaaaaccg cttacattgc caaacaacgt caaattagct tcgtgaaatc tcacttttct
    51301 cgtcaactgg aagaacgtct ggggctgatc gaagtccagg cgccgattct tagccgtgtg
    51361 ggggatggca cgcaggataa cttgtcgggc tgtgaaaaag cggtgcaggt aaaagtgaaa
    51421 gctctgcctg atgcccagtt cgaagtggtt cattcactgg cgaagtggaa acgtcagacc
    51481 ttagggcaac acgacttcag cgcgggcgaa gggctgtaca cgcacatgaa agcccttcgc
    51541 cccgatgaag accgtctttc tccgttgcac tcggtctatg ttgaccagtg ggactgggaa
    51601 cgcgtaatgg gcgacggtga gcgtcaattc tcgactctga aaagcacggt agaggcgatc
    51661 tgggcgggaa ttaaagcaac cgaagctgcg gttagcgaag agtttggcct ggcaccgttc
    51721 ctgccggatc agatccactt cgtacacagc caggagttac tgtctcgtta tccggatctt
    51781 gatgccaaag ggcgtgagcg ggcgatagcg aaagatcttg gcgcggtatt ccttgtcggg
    51841 attggcggca agctgagcga tggtcatcgc cacgacgtgc gcgcaccgga ttatgatgac
    51901 tggagcaccc cgtcagagct gggccatgcg ggtctgaacg gcgatattct ggtgtggaac
    51961 ccggtactgg aagatgcgtt tgagctttcc tccatgggga tccgtgtaga tgccgacacg
    52021 ctgaagcatc aactggcgct gaccggtgac gaagatcgcc tggagctgga gtggcatcag
    52081 gcgctgctgc gcggtgaaat gccgcagacc atcggcggcg gtatcggcca gtctcgtttg
    52141 actatgctgc tgctgcaact gccgcatatc ggccaggttc agtgtggagt atggccagct
    52201 gctgttcgcg agagcgtccc ttctctgctg taataattta tcgccgccag cgtctgagca
    52261 ggcggcttcg catcccggta tcaaagcgcc agatatgatc gaaaatgcgc atgatgccgg
    52321 gtttgccgtg tgccgacatc gccacggcat gaaagcgatg ctgatgtacc cgctgcagct
    52381 ctttcacttt actcgtcacg tcgtcaggca accgctgagc gataaaatca gaaatcacca
    52441 ccgcatcggc atcaaaccat tccctgcttt gcaagcgttc cataatggcg cgaaaacaac
    52501 tggcaagatc ggtgccgcca cgaaactgct ggcttaaaaa acggattgct tgttcgatgc
    52561 cttgtgggcc tgaaagctca taacggacga tctcggtgga aaatagcata atatagcagc
    52621 gccggttttc tgcgagagca atgcgcatca aggccaggca gaacgctttc gcacactgtt
    52681 cattaaagcc gcccattgag ccggaagtat ccacacagac aataaacggc ccgcgcggct
    52741 gttcatcgta atctttatgt accaccggac gttcgatcac tttttcacgc cacgactcac
    52801 cgtgcaggcg ataggtgagc aactgttttt ccaccagccg acggtaaaac tcatactcca
    52861 gttccgttat ccctagtgtc gccagttctg gcggcaggag acgtaaaata tcatcgcttt
    52921 gttgcagacc atcaacctgc tcaggaaccg tcgccggttc gcgcaccatg gtgcggaagg
    52981 tttccatctg cgcatcgttg cgcggtattg atttggcttc ccgagaacgc cccagctgct
    53041 ctgccaggcg tttcagttcc ggctgttcgt taagaaattc accgtatttc acaatcaact
    53101 gatagtcgcc acgtttaagc tgaccggcgc tcatatccca cagacgacca gctgcagtat
    53161 tgttatctgc gagaatcggt tcaagttgtc cgctcagcgt catgcgttcc tgaacttcac
    53221 tcaacagttg ttcgcgttct tcttctaata gctgttgatt taacgtcgtt gcttgcacga
    53281 tcagacttaa acgccaacgc tggagaaaaa gcgtgtgtaa cgctgaagtg atcgtgctgt
    53341 tagcatcaac caactgtcgg gcttgttctg cccatggaga attcagacga tgcagtaagt
    53401 ccaggatctg tggtagctgc acaataaact gtggcgtgga gaggagctgg ctttgctgat
    53461 agcacatcac ctcttcggtg agttccggcg ggactcgggc atctttcagc cgactgcgca
    53521 gcgcttcacg ccagcgggga acatcatcag tgattgccgc cttcaggcgt gggaattttt
    53581 caaagaagac cgccagctgc ggtgaggcca gcagtgcgat gatcatctct tcgatcaatc
    53641 cctcttcgct gacggccagc atcacattaa gcgtatccag cgttagcatt gttgtgcctg
    53701 gcgaatctgt gcaccgacat cctgtaggct ggcttcaatg cgacctaacc agtcgccagg
    53761 gataaacaga catttttgct gttcgctgaa taaagcgtgt tgcttgcgcc agtcgctttc
    53821 aagctcttcc agttgttgtt ttatttcacc aggcagacct tcagccgatg aaccgggaag
    53881 tgccagcgta ctgccttgta aacttacatc gcgaacgaca agatgttggg cgctatcaac
    53941 ttccagattc agtttctggg caaagccgat accgttcagt ttaccgcgaa tttcaccacc
    54001 tttgctcagc cactgttcca gcgcgctacg ctcaaaggag atatgaacca cttccatatc
    54061 atgcagtttt aacggttttt gcagcagtag agtcagagtg gaagcagtaa cgttaacagg
    54121 aagttgatac tgctgacgac ggctgaaaat gccgcccaga cgaattaccg ttaaggctgt
    54181 tttatcgctt tgttgctgct ggagttgcag gtgacgttgc acaatcgcgc caaggcgagt
    54241 caacatccct tgctgttgcc aggcgtgacc ggtcatcaat acatcaattt gttgttgtat
    54301 caaattcagg ctttgcgcgt cataccacag gcaatctttc agcaaaatga gatctaccgg
    54361 ggcaacagca ctgcgaccgc taaaaaaggc gctggcctgc aataatcgga tcgctttttt
    54421 ccagcgacga tccgagacat aaggcgcatc cggtaattta tccagttgct ggcgcagcat
    54481 aaaaatcagc tcaaatacat gatcgggcag cgtaatttca ccaatctctt tctgccagcg
    54541 ttcatattct tcatctgtga cctgcaaggc atcaggaacc ggattgtcgt tttcatcctg
    54601 ttgactggtc agcatggagc ggaaattcgc tttatcctgc actttatcta accacagacg
    54661 aatcagcatg cggtcatata acgcttccag actgctgtct gcttccggca gctcgttgga
    54721 ggccgccacc agcagacgca tcgggatttt ttctacgtgt gcaccgttgc ggaactggcg
    54781 ctcgttaatg gcggtgagca aggtattaag aattgccggg cccgctttcc agatctcatc
    54841 cagaaagaca atttctgctt ccggcaggta accgctggtt aaacgttcat agcgcccttc
    54901 atcttttagc gcctgaatag aaaggggacc aaaaacttct tccggcgtgg agaagcgggt
    54961 catcagatat tcaaacgcgc gggcattctg aaaggcgaat tttaagcgcc gggcgatcaa
    55021 acttttggca atacctggcg ggccaaggag gaacacactt tcaccactta atgccgctaa
    55081 taaacacaag cggatggcgt ggctacgttc ataaagcccc ttttccaacg aactgctcag
    55141 gcgggaaatt ctttccgcta ataaatgagg gtgagccata atgaagtggc gtcctttcgt
    55201 caaaagttct gcgtaaattg cgagtataga cgtttcttgc tggtggctaa aatagtctca
    55261 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
    55321 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
    55381 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
    55441 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
    55501 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
    55561 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
    55621 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
    55681 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
    55741 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
    55801 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
    55861 ttatttatga ttcaaaaaca tggcacggct atggtcggta agctgtttgc gccgatcatg
    55921 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
    55981 gtgctgcatg cgctgaatcc aatgtgggcg gtgcatttct tccttgaata caaaacggtt
    56041 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
    56101 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
    56161 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
    56221 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
    56281 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
    56341 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
    56401 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
    56461 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
    56521 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
    56581 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
    56641 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
    56701 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
    56761 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
    56821 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
    56881 aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct gcgtaccgaa
    56941 gacgcgccat atgtccataa tgtccgtcgg gtacagattg aacaactgtc gcccactttc
    57001 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
    57061 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
    57121 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
    57181 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaattccgcc aaacagggtt
    57241 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctc cataaggggg
    57301 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
    57361 aacgtcacga tggttttccc aactcagtca ggattaaact gcgggtcagc gaaacgtttc
    57421 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
    57481 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
    57541 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
    57601 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
    57661 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
    57721 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
    57781 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
    57841 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
    57901 ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgcgtga
    57961 tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
    58021 tctatgctcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
    58081 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
    58141 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
    58201 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgcgct
    58261 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
    58321 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
    58381 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
    58441 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
    58501 tttttcgtga tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
    58561 tgattgagat gatggtgggt cgcaagctgg aagatcaata tccgcacctg gacaaagcgc
    58621 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
    58681 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
    58741 aactgatgaa agtgctctac ggcgcactac cgcgcaccag cggttacgtc accctggatg
    58801 ggcatgaagt cgttaaccgt tcaccgcagg atggcttggc aaacggcatt gtgtatatct
    58861 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
    58921 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
    58981 aggctgtgag tgatttcatt cgtctgttta atgtgaaaac tccgtcgatg gaacaggcaa
    59041 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
    59101 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
    59161 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
    59221 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
    59281 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
    59341 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
    59401 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
    59461 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
    59521 ctccagcaaa cctcagtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
    59581 tcgggcatcg acttatcggt aggttctctg ttggcgctga ccggcgcagt tgctgcatct
    59641 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
    59701 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct
    59761 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
    59821 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
    59881 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
    59941 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
    60001 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
    60061 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
    60121 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
    60181 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
    60241 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
    60301 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
    60361 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
    60421 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
    60481 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg
    60541 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
    60601 gttcgcggca caaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct
    60661 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
    60721 ggtgaagtgg tgagccacat tgcttctgat aacgtactgg gcggcaaaat cgctggtgat
    60781 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
    60841 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
    60901 aatgttcttg ccagccagcc agcagatttt gatcgcacta aaggtttgaa cgtaatgcag
    60961 aacctgttga ccgctcatcc ggatgttcag gctgtattcg cgcagaatga tgaaatggcg
    61021 ctgggcgcgc tgcgcgcact gcaaactgcc ggtaaatcgg atgtgatggt cgtcggattt
    61081 gacggtacac cggatggcga aaaagcggtg aatgatggca aactagcagc gactatcgct
    61141 cagctacccg atcagattgg cgcgaaaggc gtcgaaaccg cagataaagt gctgaaaggc
    61201 gagaaagttc aggctaagta tccggttgat ctgaaactgg ttgttaagca gtagttttaa
    61261 tcaggttgta tgacctgatg gtgacataaa tacgtcatcg acagatgaac gtgtaatata
    61321 aagaaaagca gggcacgcgc caccctaaca cggtggcgca ttttatggac atcccgaata
    61381 tgcaaaacgc aggcagcctc gttgttcttg gcagcattaa tgctgaccac attcttaatc
    61441 ttcaatcttt tcctactcca ggcgaaaccg taaccggtaa ccactatcag gttgcatttg
    61501 gcggcaaagg cgcgaatcag gctgtggctg ctgggcgtag cggtgcgaat atcgcgttta
    61561 ttgcctgtac gggtgatgac agcattggtg agagcgttcg ccagcagctc gccactgata
    61621 acattgatat tactccggtc agcgtgatca aaggcgaatc aacaggtgtg gcgctgattt
    61681 ttgttaatgg cgaaggtgag aatgtcatcg gtattcatgc cggcgctaat gctgcccttt
    61741 ccccggcgct ggtggaagcg caacgtgagc gtattgccaa cgcgtcagca ttattaatgc
    61801 agctggaatc accactcgaa agtgtgatgg cagcggcgaa aatcgcccat caaaataaga
    61861 ctatcgttgc gcttaacccg gctccggctc gcgaacttcc tgacgaactg ctggcgctgg
    61921 tggacattat tacgccaaac gaaacggaag cagaaaagct caccggtatt cgtgttgaaa
    61981 atgatgaaga tgcagcgaag gcggcgcagg tactgcatga aaaaggtatc cgtactgtac
    62041 tgattacttt aggaagtcgt ggtgtatggg ctagcgtgaa tggtgaaggt cagcgcgttc
    62101 ctggattccg ggtgcaggct gtcgatacca ttgctgccgg agataccttt aacggtgcgt
    62161 taatcacggc attgctggaa gaaaaaccat tgccagaggc gattcgtttt gcccatgctg
    62221 ccgctgcgat tgccgtaaca cgtaaaggcg cacaaccttc cgtaccgtgg cgtgaagaga
    62281 tcgacgcatt tttagacagg cagaggtgac gcttggctac aatgaaagat gttgcccgcc
    62341 tggcgggcgt ttctacctca acagtttctc acgttatcaa taaagatcgc ttcgtcagtg
    62401 aagcgattac cgccaaagtt gaagcggcga ttaaagaact caattacgcg ccatcagctc
    62461 tggcgcgtag cctcaaactc aatcaaacac ataccattgg catgttgatc actgccagta
    62521 ccaatccttt ctattcagaa ctggtgcgtg gcgttgaacg cagctgcttc gaacgcggtt
    62581 atagtctcgt cctttgcaat accgaaggcg atgaacagcg gatgaatcgc aatctggaaa
    62641 cgctgatgca aaaacgcgtt gatggcttgc tgttactgtg caccgaaacg catcaacctt
    62701 cgcgtgaaat catgcaacgt tatccgacag tgcctactgt gatgatggac tgggctccgt
    62761 tcgatggcga cagcgatctt attcaggata actcgttgct gggcggagac ttagcaacgc
    62821 aatatctgat cgataaaggt catacccgta tcgcctgtat taccggcccg ctggataaaa
    62881 ctccggcgcg cctgcggttg gaaggttatc gggcggcgat gaaacgtgcg ggtctcaaca
    62941 ttcctgatgg ctatgaagtc actggtgatt ttgaatttaa cggcgggttt gacgctatgc
    63001 gccaactgct atcacatccg ctgcgtcctc aggccgtctt taccggaaat gacgctatgg
    63061 ctgttggcgt ttaccaggcg ttatatcagg cagagttaca ggttccgcag gatatcgcgg
    63121 tgattggcta tgacgatatc gaactggcaa gctttatgac gccaccatta accactatcc
    63181 accaaccgaa agatgaactg ggggagctgg cgattgatgt actcatccat cggataaccc
    63241 agccgaccct tcagcaacaa cgattacaac ttactccgat tctgatggaa cgcggttcgg
    63301 cttagattta cgctgtcttt tgatcaaatt attaccatcg gttgttttca gaagcatgaa
    63361 cattgctgct gaagcaacag taataatgcc cattgtgata aacgtatagt ggaattgttc
    63421 gacagtcgtt gtgccttcca ttccttcata aacgcgaagg acggccgcac ttacagcaac
    63481 gcctaaacta atcgacagtt gctgcgtgac cgccagaaca ctgttaccgc tgctggcatt
    63541 gtcatcggtc agatcggcaa gtgtgatggt attcatcgcg gtaaactgcg tcgacatagc
    63601 catccctaat ataaacaacg gcaagatcag catccatata gccattgctg gtgattgcaa
    63661 agagaactga gcgatcatta gcccaataat caccgtgatc cccactaacg tatggcgata
    63721 gcccagacga cgtaagactt gggtaaccat cgattttgca ataatggaac ctaacgctgt
    63781 cggtgccatc atacagccag caataaacgc ctgataacca aatcctacct gtaacatcaa
    63841 tggcataagg aacggtacac agccggtccc cagacgggtt gcaatattgc ctacgatacc
    63901 gatcgagaaa gtgcgggttt taaataaatc taatgaaatt aatgggtttg gcgtgcgtcg
    63961 tgcatggaga atatagagaa gcagtaaccc gatgctggtg acaattaccg tcaaggcaat
    64021 ccagctggcg acaatctttt ccccgaatag ctctattccg cttgagaaga gaacaaggct
    64081 gaggccaaac agcaaaaagc cagtgatatc gaatctgcgt cgtgcggtgg tgaaattggg
    64141 catatgtttg cgcgcgtaaa gaaggcccgc aatacctatg gggatattga ttaaaaatat
    64201 ccagtgccag gttgcccagg tgaccagcac gccgccaaga acggggccta aaattggccc
    64261 caccagaccc ggcatggcga caaaattcaa tactggaaga agttcattac gaggataagc
    64321 gcgcagtaag gccagccgag caacaggcat catcattgcg ccgcctatcc cctgaataac
    64381 ccggaagaca accagctgtg gtagcgaatt agaaagtgcg caggccagag aacccaatgt
    64441 gaacagactc acggcaaggg taaaaatgcg acgcgtaccg aagcgatcgg ctagccatcc
    64501 gcttaccgga ataagcatcg ccaccgtcag cgtataactg atgatggctg attgcatcgc
    64561 gagaggagaa cgattaaggc tatgagcgat tgcgggtaag gcggtattaa gaatagtggc
    64621 atcaagtgcc tgcatgaaga aggccatcgc cgcgatccac ggcaaacccg ccatactgcg
    64681 cttcttttta tcgctcattc aatgtcctgt tatcgggtta tcacttatca ggtgagcgta
    64741 gcagcgcctg acaagcttta aatgccgcgt cgccatcgct ttggataatc gcatcgacaa
    64801 tcgcctgatg cagatccagc tttatcactg tgtcgctggt aattgacgtg aagtaagtgt
    64861 gataaaccga atggaatagc gaggcgaatg aggtcaaaaa cggattggcg ctcatttcat
    64921 agatatgctc atgccaggcc atatcgactt cgatccagcg ttcacggcga aagttctctt
    64981 ttaatgccgc catttcggcc attaacgtat tgagatgcgc tttctgttcc gcggtgccaa
    65041 ccgttgctgc cagtaggcag gcttgcggct ccagacagat acgcataacc agaaagtgat
    65101 cgatgacctg atgaaagttc tcttctgtca tccaccaggt aagcaattcc tgatcaagaa
    65161 aattccagtt cgattgtggc atgacccgag taccaattcg cggtcgcggt aaaaccatcc
    65221 cttttgccgt taacgttttg accgcttcgc gtaccgctgt acgactcact ccaaattgct
    65281 cgcccagctc aatctcaccg ggcaaaatgg tgccgggttc atattcacct tttaagatcc
    65341 gttgcgccag cttctcagcc agaacatacg aaaggttttt ctgtgcagct aactgttgtg
    65401 cgcttaaagg cattacttat cttccttttt ctttttattc ctccttagta tgccaccagg
    65461 aagtgtgatt acggttgcaa aaacggcaaa ttgcttgttt tatggcacat taacggggct
    65521 tttgct
//
LOCUS       DAJOSY010000011        65238 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJOSY010000011 DAJOSY010000000
VERSION     DAJOSY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.011"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            140..772
                     /gene="comR"
                     /locus_tag="OHN27_000777"
     CDS             140..772
                     /gene="comR"
                     /locus_tag="OHN27_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCQ2285274.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(834..1373)
                     /locus_tag="OHN27_000778"
     CDS             complement(834..1373)
                     /locus_tag="OHN27_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCQ2285275.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1600..2904)
                     /gene="ndh"
                     /locus_tag="OHN27_000779"
     CDS             complement(1600..2904)
                     /gene="ndh"
                     /locus_tag="OHN27_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCQ2285276.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3312..3854)
                     /locus_tag="OHN27_000780"
     CDS             complement(3312..3854)
                     /locus_tag="OHN27_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2285277.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3877..4902)
                     /gene="nagZ"
                     /locus_tag="OHN27_000781"
     CDS             complement(3877..4902)
                     /gene="nagZ"
                     /locus_tag="OHN27_000781"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCQ2285278.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDSRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4913..5737)
                     /gene="thiK"
                     /locus_tag="OHN27_000782"
     CDS             complement(4913..5737)
                     /gene="thiK"
                     /locus_tag="OHN27_000782"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCQ2285279.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5718..6359)
                     /gene="lpoB"
                     /locus_tag="OHN27_000783"
     CDS             complement(5718..6359)
                     /gene="lpoB"
                     /locus_tag="OHN27_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCQ2285280.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6373..6750)
                     /locus_tag="OHN27_000784"
     CDS             complement(6373..6750)
                     /locus_tag="OHN27_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCQ2285281.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6753..7112)
                     /gene="hinT"
                     /locus_tag="OHN27_000785"
     CDS             complement(6753..7112)
                     /gene="hinT"
                     /locus_tag="OHN27_000785"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCQ2285282.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7446..9635
                     /gene="fhuE"
                     /locus_tag="OHN27_000786"
     CDS             7446..9635
                     /gene="fhuE"
                     /locus_tag="OHN27_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCQ2285283.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTNTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITSTYQF"
     gene            complement(9695..11128)
                     /gene="ptsG"
                     /locus_tag="OHN27_000787"
     CDS             complement(9695..11128)
                     /gene="ptsG"
                     /locus_tag="OHN27_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCQ2285284.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11423..12220)
                     /locus_tag="OHN27_000788"
     CDS             complement(11423..12220)
                     /locus_tag="OHN27_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCQ2285285.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12231..13235)
                     /gene="holB"
                     /locus_tag="OHN27_000789"
     CDS             complement(12231..13235)
                     /gene="holB"
                     /locus_tag="OHN27_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCQ2285286.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13232..13873)
                     /locus_tag="OHN27_000790"
     CDS             complement(13232..13873)
                     /locus_tag="OHN27_000790"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCQ2285287.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13863..14885)
                     /gene="yceG"
                     /locus_tag="OHN27_000791"
     CDS             complement(13863..14885)
                     /gene="yceG"
                     /locus_tag="OHN27_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCQ2285288.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14888..15697)
                     /gene="pabC"
                     /locus_tag="OHN27_000792"
     CDS             complement(14888..15697)
                     /gene="pabC"
                     /locus_tag="OHN27_000792"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCQ2285289.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15817..17058)
                     /gene="fabF"
                     /locus_tag="OHN27_000793"
     CDS             complement(15817..17058)
                     /gene="fabF"
                     /locus_tag="OHN27_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCQ2285290.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEVCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17146..17382)
                     /gene="acpP"
                     /locus_tag="OHN27_000794"
     CDS             complement(17146..17382)
                     /gene="acpP"
                     /locus_tag="OHN27_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCQ2285291.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17593..18327)
                     /gene="fabG"
                     /locus_tag="OHN27_000795"
     CDS             complement(17593..18327)
                     /gene="fabG"
                     /locus_tag="OHN27_000795"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCQ2285292.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18340..19269)
                     /gene="fabD"
                     /locus_tag="OHN27_000796"
     CDS             complement(18340..19269)
                     /gene="fabD"
                     /locus_tag="OHN27_000796"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCQ2285293.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19285..20238)
                     /gene="fabH"
                     /locus_tag="OHN27_000797"
     CDS             complement(19285..20238)
                     /gene="fabH"
                     /locus_tag="OHN27_000797"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCQ2285294.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20306..21376)
                     /gene="plsX"
                     /locus_tag="OHN27_000798"
     CDS             complement(20306..21376)
                     /gene="plsX"
                     /locus_tag="OHN27_000798"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCQ2285295.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21457..21630)
                     /gene="rpmF"
                     /locus_tag="OHN27_000799"
     CDS             complement(21457..21630)
                     /gene="rpmF"
                     /locus_tag="OHN27_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCQ2285296.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21682..22203)
                     /gene="yceD"
                     /locus_tag="OHN27_000800"
     CDS             complement(21682..22203)
                     /gene="yceD"
                     /locus_tag="OHN27_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCQ2285297.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22402..22986
                     /gene="yceF"
                     /locus_tag="OHN27_000801"
     CDS             22402..22986
                     /gene="yceF"
                     /locus_tag="OHN27_000801"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCQ2285298.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23098..24057)
                     /gene="rluC"
                     /locus_tag="OHN27_000802"
     CDS             complement(23098..24057)
                     /gene="rluC"
                     /locus_tag="OHN27_000802"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCQ2285299.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24630..27815
                     /gene="rne"
                     /locus_tag="OHN27_000803"
     CDS             24630..27815
                     /gene="rne"
                     /locus_tag="OHN27_000803"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCQ2285300.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEQAEPVVEPQEETADIEEVVETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(28011..28964)
                     /gene="flgL"
                     /locus_tag="OHN27_000804"
     CDS             complement(28011..28964)
                     /gene="flgL"
                     /locus_tag="OHN27_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCQ2285301.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28976..30619)
                     /gene="flgK"
                     /locus_tag="OHN27_000805"
     CDS             complement(28976..30619)
                     /gene="flgK"
                     /locus_tag="OHN27_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCQ2285302.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30685..31626)
                     /gene="flgJ"
                     /locus_tag="OHN27_000806"
     CDS             complement(30685..31626)
                     /gene="flgJ"
                     /locus_tag="OHN27_000806"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCQ2285303.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31626..32723)
                     /gene="flgI"
                     /locus_tag="OHN27_000807"
     CDS             complement(31626..32723)
                     /gene="flgI"
                     /locus_tag="OHN27_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCQ2285304.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMHVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32735..33433)
                     /gene="flgH"
                     /locus_tag="OHN27_000808"
     CDS             complement(32735..33433)
                     /gene="flgH"
                     /locus_tag="OHN27_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCQ2285305.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33486..34268)
                     /gene="flgG"
                     /locus_tag="OHN27_000809"
     CDS             complement(33486..34268)
                     /gene="flgG"
                     /locus_tag="OHN27_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCQ2285306.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34440..35195)
                     /gene="flgF"
                     /locus_tag="OHN27_000810"
     CDS             complement(34440..35195)
                     /gene="flgF"
                     /locus_tag="OHN27_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCQ2285307.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35215..36420)
                     /gene="flgE"
                     /locus_tag="OHN27_000811"
     CDS             complement(35215..36420)
                     /gene="flgE"
                     /locus_tag="OHN27_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCQ2285308.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGSAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36445..37140)
                     /gene="flgD"
                     /locus_tag="OHN27_000812"
     CDS             complement(36445..37140)
                     /gene="flgD"
                     /locus_tag="OHN27_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCQ2285309.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTILAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37152..37556)
                     /gene="flgC"
                     /locus_tag="OHN27_000813"
     CDS             complement(37152..37556)
                     /gene="flgC"
                     /locus_tag="OHN27_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCQ2285310.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37560..37976)
                     /gene="flgB"
                     /locus_tag="OHN27_000814"
     CDS             complement(37560..37976)
                     /gene="flgB"
                     /locus_tag="OHN27_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCQ2285311.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38132..38791
                     /gene="flgA"
                     /locus_tag="OHN27_000815"
     CDS             38132..38791
                     /gene="flgA"
                     /locus_tag="OHN27_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCQ2285312.1"
                     /translation="MLAIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38867..39160
                     /gene="flgM"
                     /locus_tag="OHN27_000816"
     CDS             38867..39160
                     /gene="flgM"
                     /locus_tag="OHN27_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCQ2285313.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39165..39581
                     /gene="flgN"
                     /locus_tag="OHN27_000817"
     CDS             39165..39581
                     /gene="flgN"
                     /locus_tag="OHN27_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCQ2285314.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39621..41156)
                     /gene="murJ"
                     /locus_tag="OHN27_000818"
     CDS             complement(39621..41156)
                     /gene="murJ"
                     /locus_tag="OHN27_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCQ2285315.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVMKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41266..42189)
                     /locus_tag="OHN27_000819"
     CDS             complement(41266..42189)
                     /locus_tag="OHN27_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCQ2285316.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42191..42838)
                     /locus_tag="OHN27_000820"
     CDS             complement(42191..42838)
                     /locus_tag="OHN27_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCQ2285317.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42849..43433)
                     /gene="rimJ"
                     /locus_tag="OHN27_000821"
     CDS             complement(42849..43433)
                     /gene="rimJ"
                     /locus_tag="OHN27_000821"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCQ2285318.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43669..44877
                     /gene="mdtH"
                     /locus_tag="OHN27_000822"
     CDS             43669..44877
                     /gene="mdtH"
                     /locus_tag="OHN27_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCQ2285319.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44941..45588
                     /gene="grxB"
                     /locus_tag="OHN27_000823"
     CDS             44941..45588
                     /gene="grxB"
                     /locus_tag="OHN27_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCQ2285320.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45722..46282
                     /locus_tag="OHN27_000824"
     CDS             45722..46282
                     /locus_tag="OHN27_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2285321.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46388..47434
                     /gene="pyrC"
                     /locus_tag="OHN27_000825"
     CDS             46388..47434
                     /gene="pyrC"
                     /locus_tag="OHN27_000825"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCQ2285322.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESTCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47508..47753
                     /gene="dinI"
                     /locus_tag="OHN27_000826"
     CDS             47508..47753
                     /gene="dinI"
                     /locus_tag="OHN27_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCQ2285323.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            48043..48297
                     /gene="bssS"
                     /locus_tag="OHN27_000827"
     CDS             48043..48297
                     /gene="bssS"
                     /locus_tag="OHN27_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCQ2285324.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48412..49530
                     /gene="solA"
                     /locus_tag="OHN27_000828"
     CDS             48412..49530
                     /gene="solA"
                     /locus_tag="OHN27_000828"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCQ2285325.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            49578..49691
                     /locus_tag="OHN27_000829"
     CDS             49578..49691
                     /locus_tag="OHN27_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCQ2285326.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49952..50518
                     /locus_tag="OHN27_000830"
     CDS             49952..50518
                     /locus_tag="OHN27_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCQ2285327.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAARAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50522..51097
                     /locus_tag="OHN27_000831"
     CDS             50522..51097
                     /locus_tag="OHN27_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCQ2285328.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(51139..52191)
                     /locus_tag="OHN27_000832"
     CDS             complement(51139..52191)
                     /locus_tag="OHN27_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCQ2285329.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLCIPDPTE"
     gene            52416..53336
                     /gene="lpxL"
                     /locus_tag="OHN27_000833"
     CDS             52416..53336
                     /gene="lpxL"
                     /locus_tag="OHN27_000833"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCQ2285330.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53508..54734
                     /gene="mdtG"
                     /locus_tag="OHN27_000834"
     CDS             53508..54734
                     /gene="mdtG"
                     /locus_tag="OHN27_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HCQ2285331.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            54817..55191
                     /locus_tag="OHN27_000835"
     CDS             54817..55191
                     /locus_tag="OHN27_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCQ2285332.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(55192..55419)
                     /locus_tag="OHN27_000836"
     CDS             complement(55192..55419)
                     /locus_tag="OHN27_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCQ2285333.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(55592..58135)
                     /gene="mdoH"
                     /locus_tag="OHN27_000837"
     CDS             complement(55592..58135)
                     /gene="mdoH"
                     /locus_tag="OHN27_000837"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCQ2285334.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(58128..59663)
                     /gene="mdoG"
                     /locus_tag="OHN27_000838"
     CDS             complement(58128..59663)
                     /gene="mdoG"
                     /locus_tag="OHN27_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCQ2285335.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYETPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            60057..61213
                     /gene="mdoC"
                     /locus_tag="OHN27_000839"
                     /pseudo
     CDS             60057..61213
                     /gene="mdoC"
                     /locus_tag="OHN27_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
     gene            complement(61221..62702)
                     /gene="clsC"
                     /locus_tag="OHN27_000840"
     CDS             complement(61221..62702)
                     /gene="clsC"
                     /locus_tag="OHN27_000840"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCQ2285336.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(62644..63177)
                     /gene="ymdB"
                     /locus_tag="OHN27_000841"
     CDS             complement(62644..63177)
                     /gene="ymdB"
                     /locus_tag="OHN27_000841"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCQ2285337.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(63272..63583)
                     /locus_tag="OHN27_000842"
     CDS             complement(63272..63583)
                     /locus_tag="OHN27_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2285338.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEY
                     N"
     gene            complement(63704..64036)
                     /gene="csgC"
                     /locus_tag="OHN27_000843"
     CDS             complement(63704..64036)
                     /gene="csgC"
                     /locus_tag="OHN27_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCQ2285339.1"
                     /translation="MNTLLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(64095..64550)
                     /gene="csgA"
                     /locus_tag="OHN27_000844"
     CDS             complement(64095..64550)
                     /gene="csgA"
                     /locus_tag="OHN27_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCQ2285340.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(64591..65046)
                     /gene="csgB"
                     /locus_tag="OHN27_000845"
     CDS             complement(64591..65046)
                     /gene="csgB"
                     /locus_tag="OHN27_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCQ2285341.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT        15938 a        17339 c        16008 g        15953 t
ORIGIN      
        1 aacaatcatt aattaattta aaaatcttta aatttcagtt aaatagaaag caattatttt
       61 tatgggatct ggactggtga acggaggtga ttatttttat aataaccttt cattcacagt
      121 caccaggtac aacggcatca tggcaactga ctcaacacaa tgtgtaaaaa aaagccgtgg
      181 ccgcccaaaa gtgttcgaca gggatgccgc gcttgataag gccatgaaat tgttctggca
      241 acacggttat gaagcgactt ctcttgcgga cctcgtcgaa gcgaccggag ccaaagcgcc
      301 cacgctatac gcggaattta ccaacaaaga ggggttattt cgtgccgttc tcgaccgcta
      361 tatcgatcgt tttgccgcta agcatgaagc acagctgttt tgtgaagaga aaagcgtgga
      421 gtctgcgctg gctgactatt ttgctgccat cgccaactgc tttaccagca aagacacccc
      481 ggcaggctgc ttcatgatca acaactgcac caccctctcc ccagattcag gagatatcgc
      541 caatacgttg aaatcacgcc atgcgatgca agagcgcact ttgcagcagt ttttatgtca
      601 acgacaagcg cgcggggaaa tcccgaccca ctgtgacgtg acacatctgg cagaattcct
      661 taattgtatt attcagggga tgtcgatcag cgcacgcgaa ggtgcatcgc tggaaaaact
      721 aatgcagatt gccggaacga ctttgcgttt atggcccgaa ctggtgaaat aaggcgcaaa
      781 aaaagcccct ctgcctgagc gaatgagggg ccaaattgca gagtacagcc ttgttatact
      841 ttgttattca aaatcagttg cccattacta tctagcggga tctgcgtacc cggatcgcgg
      901 tccatgcgga ttttgccctg ctgatcgcca atcttatagg tcacatcata accgagcatt
      961 ttttctgact tgtcatacac cgttttacaa cgctgttgcg tagtcgtgta agtatcgctt
     1021 tcctggagag agccctggat ctggttaccg gcatatccac cacccagcgc ccccacaaca
     1081 gtggcgacat ctttaccacg accaccacca aactgatgcc ctatcacgcc gccagcaaca
     1141 gcgccgagca ccgacccggt aatgcgattt tcatcctgca ccggtcgacg atgggtcact
     1201 gtgacgttgc gacactcctg acgcggtgtt ttaaccgttt ccttgattgg ggttgcagaa
     1261 acaacctgag cgtattgcgg gccccgttca aacacgttca gactggccac tgccgctacg
     1321 cccagcgcag ctgcgacacc aatcccgata cccgccaaca ttgatttatt cacgggaaca
     1381 cctccttctt tgcagttatg ccaatcatgc aagcgaagga gaaaagcagc tatcagacag
     1441 aagatgaaaa gcgcgggaat ggctggaaat acttaaggat tcggaggagt ctgaaggaat
     1501 gcctgatgcg acgcttaacg cgtcttatca ggcctaccgc tcgtaccatt tgtccagggc
     1561 ggatgaggcg tttatgccac atccgccagt gtacgtcgat taatgcaact tcaaacgcgg
     1621 acggataacg cggttaatac tccccaccag catcattaat ccggttttaa agtaaccatg
     1681 cagcgcaatc tgatgcattc ggtatagcga gatatataca aagcgcgcaa ttcgtccttc
     1741 aatcatcatt gagccgcgcg tcaggttacc catcaggcta ccgacggtgg agaagttcga
     1801 cagcgatacc agcgaaccat gatctttata ctgataattt ttcagcggct taccgttcat
     1861 ctgcgccagt atgttgttca ttgcgcaagt cgccatctgg tgtgcagcct gagcacgcgg
     1921 cggaacaaag cccccttccg gacgcgggca tgacgcgcag tcgccaatag cgtaaatgtc
     1981 tggatcgcgg gtggtttgca gcgtcggttc caccaccagc tggttgatac ggttagtttc
     2041 aagaccaccg atatctttca ggaagtctgg cgctttgatc ccggctgccc acaccatcag
     2101 atcagcctca atatattcgc catctttagt gtgcaggccg ccttcatcag cactggtgac
     2161 catggtttgc gtcagcacgc gaacgccaag tttcgttagc tcgttgtggg ccgcagcaga
     2221 gatacgtggc ggtaatgcag gcaaaatacg ttctcccgct tctaccagcg ttacgttcag
     2281 ggcttcgttg gtcaggcctt tgtaaccgta gctgtgcagt tgcttgaccg cgttgtgcaa
     2341 ttcagcggag agttctacac ccgtcgcgcc gccgccgaca atcgcaatgt tcactttgcc
     2401 atttgcgccc aggttggcgg agtatttcag gaagagattc agcatctcct ggtgaaagcg
     2461 acgcgcctgg tgcgggttat cgaggaaaat gcagttctct ttgacacctg gcgtattgaa
     2521 atcgttagag gtgctaccca gcgccattac cagggtgtca taggcgattt tacgttccgg
     2581 aaccagcagt tcacctttct catcgcgcag ttctgcaata gtgattgttt tcgcttcacg
     2641 atcaatatca atgacggaac ccagctggaa ctggaaacca tgattgcgcg catgggccag
     2701 atagctcaac gcatcgacgc cttcatcaag cgagccagtc gccacttcgt gcagcagcgg
     2761 tttccacagg tggctgtggt tacgatcgac cagcgtaatt ttggcttttt tcttacgtcc
     2821 cagcttatgc cccagctgtg ttgccatttc cagcccacca gcaccgccgc cgacaatcac
     2881 aatctttttc aatggcgtag tcaacgtgac ccccttaaat ttattaacca attgttaatt
     2941 aatggttatt aacatagctc ttaattaaca acaggttaca gcaatgaaaa tgttcttcgc
     3001 tattgagaat aacatgaatg gtgcattggt cataccaaaa ttgatgttaa tcaagttttg
     3061 ttgctgaaaa gataggcgac tttaccgggt gagatcaaaa aatagagttt attgtttatt
     3121 atttgagcaa attcggaaga gtaatatagg gtgaagcgta tatcgagggc gtttggtcgt
     3181 cctgataaga tgcgcagcat cgcatcaggc attgatgctc gactgtcgga tgcggcgtgg
     3241 acgccttatc cgacttacag aattcggagc gcatcaggta ccggcaaggt tacctgatgc
     3301 gacgagggca tttacccgag ggttttgaac gctttaatgc gctgtaaatg cggggagata
     3361 ttcttgaatt tgtgcgtctg ctcttcgtcc cagacaatct cgtaataatg atgcaactct
     3421 tcagatgtcc gctggctgtt aagtgcttca tcattacgcg acaaaataac cagacaacga
     3481 tcgcgattct tctcgcggaa gttggtcaca cacttagtgg caatatcggc atactcttcc
     3541 gggcgatcaa tcttgccttc catgttctca taagggaaca aattagggtt gaagatcacc
     3601 tggcggatgt cgcaaagaaa accaatccgt tccgcccagt atccgcccag gccaacgccg
     3661 caaattagcg gacgctcgtc aacgttcagt tgcaacattt tgtccacttc tttaagcaga
     3721 tgctgcatat catgtttcgg atgccgcgta ctgtagctta tcaagcgtac atccgggtca
     3781 ataaactgca attgtaagac tttctcgtgg ttacccggac tgttagagtc aaaaccgtgt
     3841 aaatagataa tcatcgcttc ctcacataag ccagggttag tgacctgctt tctcttcctg
     3901 ccagcgttcg tgtaactgat tcagacgggt gctgatcgct ttccagcgag ccgagtccat
     3961 cagttcctgt cgcgaaaatg aacctttatg atacaaacgt gtaacacgtt ctgccttgat
     4021 cggtgacaga ttatctaaca cgctgacggc ccctttacga ttattgcaga ccaggatcat
     4081 atcgcaaccc gcatccagtg atgcctgccc gcgttcggca taactgccca taatcgcggc
     4141 accttccatc gataaatcgt cagagaaaat cacgccgtca aaacccagtt cctgacgcaa
     4201 aacggttttc agccagtagg gagaaccgct cgccggacgc gaatcaacat cactgtagat
     4261 cacatgcgca ggcataatgg cgtcgagttt attttcgcgg attaaggaac tgaagaccga
     4321 catatcttta gcgcgaatct ccgcttgtgg acgtggatcg cacggtgttt ctttgtgtga
     4381 gtctgccgtt actgcaccgt gtcctgggaa gtgtttcccg gtcgttttca ttccggcttc
     4441 atgcatacca tcaataaacc ggctggcaat tgccagggct ttttgtggat cggcatgata
     4501 agaacgctcg ccaatcgccg cgctgatatg cccgacatcc agcactggcg caaagctgat
     4561 atcaatatcc atagcgatca tttcgctggc catcagccaa cccgcctctt gcgccagttt
     4621 gccgccctct tccattcctg acagcgcagc gaatgattgc gccgctggca agcgggtaaa
     4681 accttcacga aaacgctgca cgcgtccacc ttcctgatct accgccacca ccagatgatt
     4741 gcgcgaagct gcgcggatct ggcgcaccag ttcacgtaac tgggcaggat catgatagtt
     4801 acgcgtaaag agaatcagcc ctcccaccag cggatgcgca agtatttcac gctcttccgc
     4861 gtccagttcg taacctttga catccaacat tactggaccc acactgctct ccttattgtt
     4921 ttattaatag ctgccgccag gtgtcatcgg ccagcctgat aaattgttga tcgccggttt
     4981 gtcgccagcg gtactcaaac caccctgctt tgagcatcag cagccaggga aaccatcgcc
     5041 tgacctgacg ccataattgc gccggataaa tcttcgcgcg agtggcatag tcattgacca
     5101 attgccggtg ctgttcagta ttttccaccc acaccgccgc cagttccagc gcgatatcac
     5161 catctccggc atactcccag tcgatgagtt ttaaccctga cgcgctatgc actaaatttc
     5221 cggcgtggac atccatatgc aatggactta agcgtaaagg ccgtggttcc cgcgctttgc
     5281 gcagacgttt taacattcgc agccaaccca ctgtccgccg cgccggatcg ctttgctgcc
     5341 agtacagttc cagtaacggc aacagcgtta ttcgccagcc aaaacgtggt tgttgatgta
     5401 gataatacag caagcctgcc agttcgttgg tatccggcaa atacgttttt accgcgccgg
     5461 gcagatagtc gactaccatc cagtcacgga gatataaatg cggcttcggt gcaatgcttg
     5521 cgggtagttg tgataaagcc cgatactggc gcaagaacgc ggactgcggc gcatcaggat
     5581 cgtgcggctg acgcacaaca aaacgctggc cctgatgttc aatgagaaaa ctcccgccac
     5641 taagcccact attaaacgtc gtgacgggat gaaactgcgg gaaaaagcgc gacagcaatt
     5701 cgtcgcgcat gatgggatta ttgctgcgaa acggcacctt tacctgacca gataatttcg
     5761 cccgtctgca ccagcatcag ctgcatttgt agggtcggag cgttaacgtt gccagaggcg
     5821 ctggagtaca gcacgtaatg agcgccgaca ttgcgggcaa tgcctatggc tttactacgg
     5881 gtacctaaac tgtcctgcgg cgacaaacct aactgttgct tagccatcga cagctgctgg
     5941 gcggaaacca gggtaaattt cccgttatta gccagcgcat ttcgcagcgt ttcggtcgct
     6001 tctgcggcat tcagcgaacc gttagtacgg ttgttaacgc tatcaaccag caggacgcta
     6061 cccgcagtca ccccgtcagc cccaagcatc ttactgacca tcggctgcat tgcgccattc
     6121 cagtcataat ggcgaatatg cggcgcaggc ggtgccgttt gatcttcgtg ctcaattggg
     6181 cctggctgct gcgggatcgt cggcaccgag ggcactgtgg ggacaggctg ttgtggctcg
     6241 gctggttgtt ccggcgctgg tttcacttct tctaccggtg caggttcacg ttgccccaca
     6301 cacccggcga gaaacatcgc cagcgcggta atcaaggcgt agcgactcat ttttgtcatc
     6361 aagattcacc ccttacaaat atagataaag tctgactttg tgcgccccca gaaaattggc
     6421 gctgccgtac agcgttaccg ccgaatgtgc gggaatgcta acgctgcgtg gcctttccag
     6481 aggatgcatc tccagccctc tggcgtcata ccagtaaaaa cgataatgaa cggtaacggg
     6541 ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt tgaatatcag acgtcgaaag
     6601 gacgggcttt tctgcactga tgcccgcagc caataaagat gactccatca ccagcgattg
     6661 ctcatcattc accggaattt ccggatgtga acgacagccc accagcagta acaacgccag
     6721 agacaccagc ccaaagcatc cttttctcat cgttacagac ctttatgcgc cagcattggt
     6781 cccagcggac ggccacccaa caagtgcata tggatgtggt aaacctcttg tccgccatgg
     6841 cggttggtat tcatgatcag acgatagcca tcttcggcaa taccttcttg ctcagcaatt
     6901 tttgccgcta cggtgatcat gcgtcccagc gcctgctcat gctcagctga gacgtcgttc
     6961 acagtcggaa tgaggatatt cggaatgatc agaatatgcg ttggcgcttg cggcgaaata
     7021 tcgcgaaacg ccgttaccag atcatcctgg tagacgatat cggaggggat ctcacgacga
     7081 ataattttgc tgaatatagt ttcttctgcc acgacatttt cctttttcaa atagccatgc
     7141 gttgcgccat gctacgccgc gccacactgc gagtatagag tatggtcgag ttaaacctgc
     7201 tctttcaacc ttaactcacg atttcttaag caaaaaatcg ttttgctgcc gtatatatcg
     7261 ccattattcc catttctgcc tgcgaatgcg ctgaaaagac attctctgtt tggtttacat
     7321 gacaaatatt aatgcgtata tttctcattt gcatttacaa acaaaattat tcgcacacta
     7381 aatataatcg ctcatcaccc tcctccggca tgagcctgac gacaacataa accaagagat
     7441 ttcaaatgct ttcaacacaa tttaacaggg ataatcaata tcaagccatc accaaaccgt
     7501 cactacttgc cggttgcata gcactggcac tattaccttc tgccgctttt gctgcaccag
     7561 ccactgaaga aacggtgatt gttgagggtt cagccacagc tccagatgat ggcgaaaatg
     7621 attacagcgt aacgtctacc tctgcgggta ccaaaatgca gatgactcaa cgtgatattc
     7681 ctcagtcggt cactattgtt agccagcagc ggatggaaga tcagcagtta caaacgctgg
     7741 gcgaagtgat ggaaaacacg ctggggatca gcaaaagtca ggcggattcc gatcgtgctc
     7801 tttattattc ccgcggattc cagatcgata actatatggt tgatggtatc cccacctatt
     7861 ttgaatcgcg ctggaatctg ggcgacgcac tttctgatat ggcactcttt gaacgcgtag
     7921 aagtagtgcg tggcgcgaca ggactcatga ccgggacggg taatccatct gcggcaatta
     7981 atatggttcg aaaacacgcg accagtcgtg aatttaaagg cgatgtctcg gcggaatacg
     8041 gtagctggaa caaagaacgg tatgtggcgg atttacaaag cccactcacc gaagacggta
     8101 aaatccgcgc gcgaattgtc ggcggctacc agaataacga ctcatggctg gaccgctaca
     8161 acagtgaaaa gaccttcttc tcgggcattg tcgatgctga tttaggcgat cttacgacgc
     8221 tttcagccgg ttacgaatat cagcgcattg atgttaatag ccctacctgg ggcggtttac
     8281 cgcgctggaa tactgatggc agcagcaaca gttacgatcg tgcacgcagt accgcacctg
     8341 actgggcgta caacgataaa gagatcaaca aggtctttat gaccctgaag cagcggtttg
     8401 ctgatacctg gcaagcgaca ctgaatgcca cccactctga agtcgaattt gacagcaaaa
     8461 tgatgtatgt cgatgcctat gtaaacaaag cggatggtat gctggttggg ccatacagta
     8521 attatggacc tggctttgat tatgtcggcg gcaccggttg gaacagtggc aaacgtaaag
     8581 ttgatgcgct ggatttgttc gctgacggta gttatgaatt gtttggtcgt cagcacaatc
     8641 taatgtttgg tggcagttac agcaaacaaa acaatcgtta cttcagttca tgggccaaca
     8701 tcttcccgga tgaaattggc agtttctaca actttaatgg caatttccca caaaccgact
     8761 ggtcaccaca gagcctggcg caggacgata ccacacatat gaaatcgtta tatgctgcca
     8821 ctcgtgtcac ccttgccgat ccgctgcatc tgatcctcgg cgcacgttat accaactggc
     8881 gggttgatac gctgacttac agcatggaga aaaaccacac cacgccttac gctggtctgg
     8941 tgtttgacat caatgacaac tggtcgacct acgccagcta tacctctatt ttccagccgc
     9001 aaaatgatcg tgacagttca ggcaaatatc tggctccaat caccggtaac aactacgagc
     9061 tgggtctgaa atcggactgg atgaatagcc gtctgaccac cacgttagcc atcttccgta
     9121 ttgagcagga taatgtcgct cagtccaccg gtacacctat ccccggcagc aacggcgaaa
     9181 ccgcctataa agcggtggat gggacagtca gtaaaggggt ggaatttgaa ctcaacggcg
     9241 caattaccga caactggcag ctgacatttg gcgcaacgcg ctatattgca gaggataacg
     9301 aaggaaacgc cgttaatcct aatctgccac gcaccacggt taaaatgttc accagctatc
     9361 ggttgcctgt catgccagag ttgacggtcg gcggtggtgt taactggcaa aatcgcgtgt
     9421 ataccaacac cgtgacaccg tatggcacct tccgcgccga gcaaggtagc tacgcgctgg
     9481 tggatctctt cacccgctac caggtgacga aaaacttctc gttacagggg aacgtcaata
     9541 acctgttcga caaaacctac gataccaacg tggaaggttc tatcgtctac ggcacaccgc
     9601 gtaatttcag cattaccagc acgtatcaat tctgaataac acctgtaaaa aaggcagcca
     9661 tctggctgcc ttagtctccc caacgtctta cggattagtg gttacggatg tactcatcca
     9721 tctcggtttt caggttatcg gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa
     9781 ccactacgcc cgctgcgccc agtttcttca ggccggcctg atccacttta gacacatcag
     9841 caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt tctttaccac
     9901 caaatgcagc aaccagagcc ggtgccattt cgctggtacc tgtcgctttt gcatcttcag
     9961 tcgcgtcttc acgacccggc gttttcagat ccagtgcttt aatcagcacg cggaagatgg
    10021 tgtagtaaac aatcgcataa ccgataccga cgatcgggaa cagccacagt ttgctgctgt
    10081 taccagacag aacgatgaag tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc
    10141 ccagaagaat acagattggg aatgccaggc ctgccagaat cgcgtggatg atgtacagga
    10201 tcggcgcaac gaacatgaag gagaactcga tcggctcggt gataccggtc aggaacgagg
    10261 tcagcgccgc ggaaatcata ataccgccca ctttcgcgcg gttttctggt ttagcagagt
    10321 gccagatagc aattgcggca gctggcagac cgtacatttt gaacaggaag ccaccagaca
    10381 gtttacccgc agtcgggtca cccgccatat aacgcggaat gtcgccgtgg aaaacctgac
    10441 ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg tacgttccag atgtggtgca
    10501 gaccaaacgg taccaggcaa cgttcgatga aaccgtaaat gccaaacgca actaccgggt
    10561 tctggtaagc agcccactga gagaaggtct ggattgcaga accaatcggc ggccaaatga
    10621 aggacagcac aacgccagta aagatggcag ccaggccaga aatgatcggc acaaagcgtt
    10681 taccggcaaa gaagccaaga tactcaggca gcttaatacg gtagaaacgg ttaaacatgt
    10741 acgctgcgat cgcaccggag ataatccctc cgagtacgcc agtatccgcc aggtgtttag
    10801 aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc atggttttaa
    10861 ccatgatgcc ataggcaaca actgcggcca gcgcggatac gccatcgtta ttggtaaagc
    10921 cgagggcgac accgatcgca aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt
    10981 ctgccataac atgcgataca acggcgggca gccagctgaa attcgcggaa ccgacgccca
    11041 gcagaatacc tgcgataggc agtacggata ccggcagcat cagcgattta ccgacctttt
    11101 gcaggttagc aaatgcattc ttaaacataa ttgagagtgc tcctgagtat gggtgctttt
    11161 tttacgttct cacgcgtggc aaggggggag agcctcgccg tgtacagggc atctaagcgc
    11221 cctttattta ttacacagag taaaataatt cagtgccaat ttgtttgacg gctatcacgt
    11281 ttcaacttca gatcattgga gaagttgcac attttgacaa aacatatgtc acaatatttc
    11341 aaacgcggtt attcaccgcc caattttcgg cggtgaactt tactcgtttt agccattaat
    11401 tacgagcttt aaaaaaactc attcaacgga tggattgaag gcgggaagcg tcgatgtgaa
    11461 acagacgggc gaagttatcg gtggttacct gcgccagttc ttcaacggca acacctttca
    11521 acacagccat gtattctgca acgtcacgaa ccatcgcagg ttgattctct tttcctcgat
    11581 gcggtaccgg cgcaaggtaa ggtgagtcag tttccaccag taatcgatcc agggggacat
    11641 aacgcgcagc atcgcgcagt tgctccgcat tacggaaggt cacaatgccg gaaaaggaga
    11701 tgtaaaatcc gagatccagt aatttacccg ccgtttctct gtcctctgta aaacagtgta
    11761 gtacgccacc gcaatccgtc actttttctt cgcgcagaat tgccagcgta tcggcacggg
    11821 cgtcacgggt atggacgata accggcttgt tcagttcacg accaatctgg atatgatgga
    11881 tgaaggactc ttgctgacgt actttagttt ccggtgtgta ataataatcc agcccggttt
    11941 cacccagcgc tacaacaccc tcttctgccg ccagacggcg taaatcttct acatcgtagg
    12001 gatcattctg gttgagtgga tgcacgccac aagaaaatac gacgttgtca cgttcgccta
    12061 ccagatcccg catatgtaaa taacccggta atgttgtggc gactgccaga caaaatttca
    12121 catcgcgtgc ggcggctttc gccagaacgt catccacgtc cttatgcaaa gattcataat
    12181 ccagaccatc gagatggcag tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga
    12241 ggaaccggta gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa aagatcggtg
    12301 atgagaagct cgcggttgat gcctgtaaca gacattaact gttcacgaat gtggcaaaca
    12361 tcccccagta tagcctgcag gcgcgaggga gaaagatggt ttgccagttc ggcgaccagg
    12421 cccggcacat caacattagt cacctgcgca gcaccatgat ggcgttttag cgcatccatc
    12481 agcaacgttg ccagccagtg taaacgcgcc ggagcttgtt catgattaag ggccgctagc
    12541 agcgaatacc agtcgcccga tggcacgcta tatgccaacg cctgacacaa tgtttcacga
    12601 gcctgccagt tatctccctg aaacaacgcc agtgccgcgc caggcgaacc ggcgcttaag
    12661 cgcaatgcgg caagtaatgc atcctgtgac attgtcactt cgcgtgaaag ccaggtcacg
    12721 gcgtactgtt ccggcggcgg cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc
    12781 agtaaacgtt caggctcgcg ggtagccagg aaaaaccagg tttctgctgg tggctcttca
    12841 agcgttttca gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc ggttacccag
    12901 acaactttcg caccacctag gcgtgcgtgc tcattcagct tttcggtgac ctcacgtacc
    12961 gcatcaatgc ccagcgtatt tttccctttt tcgggagcca gggtgtagta atcgggatgc
    13021 gtgccagcct gcatcaactg acatccacga cagtgaccgc aacttttgtg gccctgcggt
    13081 tgttggcaga gtaaataacg gctcagggcg tagattaaag catcatcgcc catgcccggt
    13141 aacgcctgaa tgagtagcgc atggtgacct cttccggcct gatagctggc taccagtttt
    13201 tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc caactccttc acccagtggg
    13261 tcacggtagt gcggattgca tccatcacgg cctccagcgg ctgggtggca tcaatggtat
    13321 gaatgctttt atcttgtgct gccagttcca gatagcgggc gcgggtgcga ttaaagaaat
    13381 cgaaagactc ttgctcaata cgatccagct cgccgcgcgc acgcgcgcgt tttaagccaa
    13441 cttccggggt aacatcgaga tagagcgtta agtcggggcg aaaatcaccg agaacagcat
    13501 cacgcagtgt tgccagcata tgttggtcaa taccacgtcc gccgccctga tatgcctgag
    13561 tggagagatc gtggcgatcg ccaatcaccc aggtgccgtt agccagagct ggtttgatga
    13621 ctgtttctac cagttgaacg cgcgcggcat aaaacatcag aacttcggct ttatcggtaa
    13681 tgacttcatc gcctaccgat ttgatatcca gcaccaggct tcttaacttt tcggcaagtt
    13741 gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat acccagttgc tcgagcgtct
    13801 caaccaccac attacgcgcg gtagttttgc ctgcgccttc cagcccctca atgacgatat
    13861 acttactgcg catttttttc cttaagcact ttcagataat cctgcacaga cttgttatgg
    13921 ctggcaagat tggtattaaa cgtgtgacca cctttaccat cggccacaaa atagagatac
    13981 ggcgtttttg ccggatgcgc agcagccttc agcgaatccg cccctggcgt agctatcgca
    14041 cccggcggca aaccggtaat ggtataggtg ttatacgctg tcggcgtttc caggtctgca
    14101 cgagaaagtt tgccattata acgctctccc atcccgtaaa tcacggttgg gtcggtctgc
    14161 aggcgcatac caatgcgtaa acggttgata aataccgagg caacctgatc gcgttcactg
    14221 gcaacggcgg tttctttttc gataattgat gccatcgtca ccagctgatt tttatcttta
    14281 taaggcagac cgtccgcacg cccttcccag gcgctatcga ccgctttcac cattttcttg
    14341 tgcgctcgct tgagtaacgc gacatcggtg gtattggcgg tatacatcca ggtgtctggc
    14401 cagaaccaac cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc tacggtggcg
    14461 tacttatcgt cactcagcgt gtgcttgata tacggagcct cacgcaattg cttgaggtag
    14521 tcgctcagac gcatcccttc taccagtcgc agcgggaact gtgcttcttt accgctttcc
    14581 aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt cccggcttta
    14641 aagtgagaaa gatccggttc gatacgcagc agccattgaa aaacccgtgg gcgattgatg
    14701 atcttatcag cataaagctg ttcaccgagc gccagacgtc cggtccctgg cttcagggta
    14761 aatatcgtct cttctttgat aagcaatttg ctgtcggcaa gatggcgaac cttccagacg
    14821 cccacaccag cggcgatacc cagtaccacc agcaataaca agattatcaa taacactttt
    14881 ttcatgacta attcgggcgc tcacaaagtg gggctaaata ttcatataac gttgctgacg
    14941 aaaaggagac atcgccacag gcacgtacgg gcatcactgg cattaacgca ttacaaataa
    15001 ccatctcatc tgcctgcaac gcctcttcca gagaagcttg cacttcgaca agctgataag
    15061 aggattgtgc cagcaaacgg atacagaatt gtcgcataat gccgttaaca cctgcctgat
    15121 ccagtcgcgg cgtataaact acgttgccct tccgccagaa caaattagcc gcacagcatt
    15181 ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat
    15241 gagagcgaat caatacttgc tcaagacgat tgagatgttt aatacctgca agatgaggat
    15301 tgcgccccag ccgcaccggg cttagcgcca acgtcatccc ctcgttacgc aaacggtcgt
    15361 aatgtgcagg ataagccgta acggagagaa tccgcgttgc cggtccactg ttcaatgtgc
    15421 tgtaccctcg cccgccacta ccgcgactga tcacgacttt cagtacacca ttttgctgtt
    15481 ctgctgccag cgttttcatc tcttgttcaa gctgtggcca gaagtcacag gaaatcatca
    15541 accgctgaca agcatcctgt agtcgctgga tatgcgccga taacaaactg actttaccgt
    15601 cgataactct ggcggtggta aaacaaccat caccaaactg cgttgcccga tcgcttactg
    15661 ccagcgattc ctgcttataa ccgttaatta agaacatacc gactccttat cagagaattg
    15721 ttagtgtggc agcatgttca ctacggaaca agtcggaata aaagctaaga aaaaaggccc
    15781 gcaagcggac cttttataag ggtggaaaat gacaacttag atctttttaa agatcaaaga
    15841 accattagtg ccaccgaagc cgaaggagtt acacagcgtg tattccattc cgctgacctg
    15901 acgtgcttca tgcggtacga aatccagatc gcaaacttca tccgggttat ccaggttgat
    15961 ggttggcgga acagcctgat cgcgcagcgc caggatggag tagatagatt ctactgcacc
    16021 cgccgcacct aacaggtgac cggtcataga tttcgtggag cttaccaaca cacggcttgc
    16081 agcttcaccg aagatggttt tcaccgcctg cgcttcagct ttatcgccag ccggcgtaga
    16141 agtaccgtgc gcgttaacgt agccaatctg actcgcttca atgcctgcat cacgcagagc
    16201 atttgccatt gccagagctg cgcctgcgcc attttctggc ggtgacgtca tatgataagc
    16261 atcgctgctc ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc gttttttcgc
    16321 gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg cccagtacga agccatcacg
    16381 ctctttatcc cacgggcggc tcgccgcttg cgggttatca ttgcgggtag ataatgcacg
    16441 tgccgcgcca aaaccaccaa cacccagcgg cgtactggct ttctctgcgc cacctgcaac
    16501 catcacgtca gcatcgccat acgcgataat acgcgcagca tggccaatgt tgtgcacgcc
    16561 ggaagtacag gcggtcgcga tagagatgct tgggccacgc aggccataca tgatagtcag
    16621 atgacctgcc accatgttca caatcgttga cggaacgaag aatgggctga tcttacgtgg
    16681 accaccgttc atcagagatg tgtggttttc ttcgatcagt ccgaggccgc caatcccgga
    16741 gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc cagaatcctg
    16801 catggcctga acgccagcga caattccata ttgaatgaag gcatccatct tgcgctgttc
    16861 tttgcgcgag ataatgtcct cacagttaaa atcctttact aagccagcaa atttcgttgc
    16921 ataggcgcta gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg caagcagagc
    16981 tttccaggta gactctacgg tattgccgac aggagacaac atgcccagtc cggtcacaac
    17041 tacacgacgc ttagacacgt ttgtcctcca gggagggaaa aaatgattct agtgggacaa
    17101 aaagataaaa ctcaggcggt cgaacgaccg cctggagatg ttcacttacg cctggtggcc
    17161 gttgatgtaa tcaatggcag cctgaacggt ggtgattttc tcagcttctt cgtccggaat
    17221 ctcagtatca aactcttctt ccagagccat taccagctca acggtgtcaa gagaatccgc
    17281 gcccaggtct tcaacgaaag aagcattgtt ggtaacttct tcctgcttaa cgcccagctg
    17341 ttcgccgata attttcttaa cgcgttcttc gatagtgctc atactcttaa atttcctatc
    17401 aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg ttgaaaaatt tgcaactaaa
    17461 tcccggcagg tcttaccacg attttacgtt attttgaggc cttacccccc aataacgcaa
    17521 atcattttgc actaattatt actcataacc acgcagactg cgcgcaacat gagcattttg
    17581 tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca tgcaaagttt cacccgtgat
    17641 gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg gcgatttcct gtgcgccgcc
    17701 gaggcgaccc gcaggaacct gcgccaggat acccgcacgc tggtcatcgc tcagcgcacg
    17761 tgtcatgtcc gtttcaataa agcccggagc aacaacgttt acagtaatac cgcgtgacgc
    17821 aacttcgcgc gccagtgatt tactgaagcc gatcaagccc gctttcgccg cagcgtagtt
    17881 ggcctgaccg ccatttccca tggtaccaac cacagaaccg atagtgataa tacgaccatg
    17941 acgctttttc atcatagcgc gcattaccgc ttttgacaga cggaaaacag atgaaaggtt
    18001 ggtttcgata atatcgttcc actcttcatc tttcattcgc attaacaggt tatcacgagt
    18061 gataccggca ttattgacca ggatatccac ttcaccaaat tccgcgcgaa ttttttccag
    18121 aacagattcg atagatgccg ggtcggtcac attcaacatc agacctttgc cgttagcacc
    18181 taaataatca ctgatcgcct gagcgccatt ttcactggtc gcagtgccaa taactttcgc
    18241 gccacgggct gcgagcgttt cagcaattgc gcggccaatt ccgcggcttg caccggttac
    18301 cagtgcgatt tttccttcaa aattcatgat tttcctcttt taaagctcga gcgccgctgc
    18361 catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg tcgacaatgc gtttcgtcag
    18421 gccagtaagc accttgcccg ggccgacttc atagagatgt tctacgcctt gcgctgccat
    18481 gtactcaaca gacttcgtcc actgaaccgg gttatacaac tgacgtacca gtgcgtcacg
    18541 gatggcatca ccattggttt cgcatttcac atcaacgtta ttcacaacag aaactgttgg
    18601 tgcgttaaag gtgattttcg ctaattctac tgccagtttg tcggctgctg gtttcatcag
    18661 cgcacagtga gacggtacgc tcactggtaa cggcagcgcg cgttttgcgc cagcagcttt
    18721 acaggcagcg ccagcacgct caaccgcttc tttatgaccg gcaataacca cctgtcccgg
    18781 agagttaaag tttaccggag aaacgacctg accttctgca gcttcttcac acgctttcgc
    18841 aatagacgca tcatccagac cgatgattgc cgccatagcg cccgtgcctt ccggtacggc
    18901 ttcttgcatg aacttgccgc gcatctcaac cagacgcacc gcatcagcga aatcaatcac
    18961 accagcgcaa accagcgcgg agtattcccc caggctgtga ccggccatca ttgccggtgc
    19021 tttaccgccc tgctgctgcc atacgcggta cagcgcaaca gatgcagtca acagcgctgg
    19081 ctgggtttgc caggttttat tcagttcttc agctggcccc tgctgggtca gcgcccacag
    19141 gtcgtagccc agcgccgcag aagcttcagc aaacgtttct tcgacaattg gatagctcgc
    19201 cgccatatca gccagcattc caacggtttg agaaccctgt ccagggaaca caaatgcaaa
    19261 ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc gcggagcccc aggtgaatcc
    19321 accgccaaag gcttcaagca gaaccaactg tcccggctta atgcgcccgt cgcgtacagc
    19381 ttcatccagc gcgcacggga cagaggccgc agaggtatta ccgtggcgat ccagcgtcac
    19441 cacgacattg tccatagaca taccgagttt tttcgccgtt gcactgataa tacgcaggtt
    19501 agcctgatgc ggaaccagcc agtccagttg agaacggtca agattattcg ccgccagcgt
    19561 ctcatcaacg atgtgcgcca gttccgttac cgcaaccttg aagacttcgt tgcccgccat
    19621 cgtcagatga attgaattct ctggattcac acggtcagca ttaggcagcg tcagcaactc
    19681 accatagcta ccgtcggcat gcagatgggt ggagatgatt cccggctctt cagaggcagc
    19741 cagcaccgca gcgcccgcgc catcgccaaa aataataata gtcccacgat cggttggatc
    19801 gcaggtgcgc gccagtacat cggaaccgac gaccagagca tacttcaccg ccccagattt
    19861 cacgtattga tcggctacgc ttaatgcata ggtgaaacct gcgcaggctg ctgcaacgtc
    19921 aaatgccggg caacctttaa tgcccagcat gctttgaatc tgacaagctg cgctcgggaa
    19981 agcgtgcgta gcagaagtcg ttgccacaac gatcaggcca atctggtctt tctcaatgcc
    20041 cgccatctca attgcgcgtg tcgccgcttc aaagcccatg gttgaaacgg tttcgtttgg
    20101 cgcggcaatg tggcgttcgc ggataccggt acgagtgaca atccactcgt cagaggtgtc
    20161 caccattttt tccaaatcgg cgtttgtccg cacttgttcg ggcagatagc tgccagtacc
    20221 aataatcttc gtatacatgt acgctcagtc acttttcggt tatataccgt cacttgcaaa
    20281 ctgcgagttc gctggcagcg tcctgctacc gcagagttcc gcttttgcca ccgtccagca
    20341 gctcaaaacc agctgggtat acagattcca ggcgagcggc aattcgctga ggaacttgtc
    20401 gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa agctcgctga ttggctgcac
    20461 catgactttt tatcaccgtg ccgcgcaatc ctaacagaca ggcgccgtta tactggtcgg
    20521 ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag ccaacgcttt aataacagta
    20581 gccaccacga ccgttttttc ccttcaccct gagatttcag cagagaaagg aacatcctga
    20641 caacaccttc catcgtcttt aacgtgacat ttcctgtaaa gccgtcacaa accagcacat
    20701 ctgtcttgcc agttaacaac tcattggctt caagatagcc gatataattg atagaaggga
    20761 ttgttttaag caccgctgag gcatcccgaa tactgtcgag accctttact tcttcttcac
    20821 caatattgag caacgccacg cgaggattgg gaatttccac cacctcttca gccagaactg
    20881 agcccataat ggcaaattgc accaacattg tgctgtcaca atcgacgttg gcccctaaat
    20941 cgaggaccac cgttttgccc ttttgctgat gtggtaatac cgtcaccagc gccggacgct
    21001 caatcccctc caggggcttg agtaataatt ttgccagccc catcagtgcc ccggtattac
    21061 cggcactgac acaggcttgc gctcgacctt ctttcaccag ctccagggcc acgcgcattg
    21121 aactcccacg actggcgcgg atagcttgcg aaggccgggc atcactggcg ataactgact
    21181 gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc agctttagca agtaatggcg
    21241 tgatggcgtc gggattgccg actaaaagaa gagtgagttg cgaattagaa ttcagtgcct
    21301 gcaatgctgc aggcactgtc acggaagggc caaaatcccc tcccatgaca tctaacgcca
    21361 gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt ctttccccag ttgcctgggg
    21421 aaaatcctca ctaagcttca tcacgcagac gcgtgattac ttagcgatga ccttgcggcc
    21481 gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt ttttcaccag aagttttgtc
    21541 tacagacagg ctggtgactg cggtcagcgc gtcatgggaa cgacgcatgc cacgtttgga
    21601 acgggttggt ttattctgtt gtacggccat ggaccttact cctcaattac ggtttatccc
    21661 cgatccaacg gggagcacca attacttacg ctttaagctg gctaatacgg caaatgggtt
    21721 tggcttttgc acttcttcag gcagttcacc aaagaccatg tccgcttcgg acacttcaca
    21781 gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg atgatttcat cttcaaccat
    21841 tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc ggttcatacg cttccggcag
    21901 tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa tacgttgtgt agacctgatg
    21961 agtaaacggc ttcccgcaac gctgacactc gagcgttacc gtcaccttcg catcgccgtt
    22021 taacactgcg agacgttggt tatcgatagc gaacgacatg gagcattcca catcactgtc
    22081 cacactgact acggattcgg cgacgcgctc aacctgatca ggggtataga taccctggta
    22141 atcaaggcgt ttttgagccg tacgaaccgg atcgagagtc aggggtaatt ttaccttttg
    22201 catagggcgc gcatattaac tttgtaacgt catagagtca aagaaaaagg cagcctgtgg
    22261 ttgccttttg ccaataattc gcacacattg cgggttactg ctttattttc gttcagtgct
    22321 gataacgaac atcaacctgc aaaacgaagc gcatagttta aaatggctat catcaatacg
    22381 ctatatctga tggaaaaaaa tatgcctaaa cttattttag cctccacctc gccatggcgt
    22441 cgcgctcttc tggaaaaact gcaaatctct tttgaatgtg cagcaccaga ggtcgacgaa
    22501 accccacgca gcgacgaatc accgcgacag ttggtgcttc gactggcaca agaaaaagcg
    22561 caatctctgg cgtcacgtta tccggatcat ttaattattg gttcagacca ggtatgtgtt
    22621 cttgatggcg aaatcaccgg taaaccgtta acggaagaaa atgcccgtct gcaattacgc
    22681 aaagccagcg gcaatatcgt caccttctat actggactgg cgttgtttaa ttcggcgaat
    22741 gggcatctgc aaacagaagt ggagcctttt gacgtccatt tccgtcacct gagcgaggcg
    22801 gagattgata attacgtgcg taaggagcat cccctgcact gcgcgggtag ctttaagagt
    22861 gaaggatttg gcattacgct gtttgagcgt ttagagggac gtgaccctaa cacgctggtt
    22921 ggtttaccgc taatcgcgct gtgtcagatg ttacgtcggg aagggaaaaa cccgctgatg
    22981 ggatagttgt ttgcgccgga tgcggttacg tcgcatccgg caatcggtgc aggcggcctg
    23041 atgcggcgta aacgccttat caggccaacc gcccattatc aggcttatat gcttgtatta
    23101 gcgcgcgtta cgcagctttt gcaaacaacg cttcaaacct tcatccatcg gcgcttcgat
    23161 acgcatcacc tcaccggtcc ccggatgggt aaacttcaac gctgcggcgt gcaggaacag
    23221 acgatttaat cccgtgcctg cttcagtgag ctgtctgtca aattcacggt caccgtagcg
    23281 atcgtcaaag gcaatcggat gacccgcata ctgtgtatgc acacggatct gatgagtacg
    23341 tccggttacc gggctacaac gcaccagggt ggcaaatgca tagcgttctt ccactttaaa
    23401 gcgtgtttcc gacggtttgc cttcctgact gacacgcacg atacgttcgc cgctttgcag
    23461 aatatttttc aataacggcg cttgaacgct cttcacatgc gactgccact gaccgcgcac
    23521 cagcgccagg taatcttttt gcatcccttt ttcacgtaat tgctcatgca gagaacgcaa
    23581 cgccgagcgt tttttcgcta ccagcaaaac acctgaggtg tcccggtcaa gacgatgaac
    23641 cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcaataa cgccgaagct
    23701 taaaccactg ccgccatgta ccgccgtacc ggaaggttta ttcagcacca ggatgtgatc
    23761 atcttcatat aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg gcgaaaccgc
    23821 ctcttcttcc cgctcagcaa cgcgaaccgg tggaatacgc acctcatcac ccgcttcgag
    23881 tttatattca ggcttaatac gttttttgtt cacccgcact tcgcctttac gcaaaatacg
    23941 gtaaatcata ctttttggta cgcctttcaa ttgggtacgc aaaaagttat cgatacgttg
    24001 ccccgcttcg tcagcggtga tagcaacaat ttttacggat ggagtctctg ttttcatggt
    24061 tggcgattct aattagccaa catgattcgc gccactcatt tttctatgct tatatttact
    24121 tttgcacctt attacttcac tgcgtgatca ctttattgat ggttattaaa ccaatcacca
    24181 gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca tcttgctata
    24241 acaaggcttg cagtggaata atgaggccgt ttccgtgtcc atccttgtta aaacaagaaa
    24301 ttttacggaa taacccattt tgcccgaccg atcatccacg cagcaatggc gtaagacgta
    24361 ttgatctttc aggcagttag cgggctgcgg gttgcagtcc ttaccggtag atggaaatat
    24421 ttctggagag taatacccag tctgtttctt tgataattgc gctgtttttc cgcatgaaaa
    24481 acgggcaacc gacactctgc gcctctttga gctgacgata accgtgaggt tggcgacgcg
    24541 actagacacg aggccatcgg ttcacacccg gaaaggcgtt actttgcccg cagcttagtc
    24601 gtcaatgtaa gaataatgag taagttacga tgaaaagaat gttaatcaac gcaactcagc
    24661 aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct gtatgacctg gatatcgaaa
    24721 gtccagggca cgagcagaaa aaggcaaaca tctacaaagg taaaatcacc cgcattgaac
    24781 cgagtctgga agctgctttt gttgattacg gcgctgaacg tcacggtttc ctcccactaa
    24841 aagaaattgc ccgcgaatat ttccctgcta actacagtgc tcatggtcgt cccaacatta
    24901 aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat cgataaagaa gagcgcggca
    24961 acaaaggcgc ggcattaacc acctttatca gtctggcggg tagctatctg gttctgatgc
    25021 cgaacaaccc gcgcgcgggt ggcatttctc gccgtatcga aggcgacgac cgtaccgaat
    25081 taaaagaagc actggcaagc cttgaactgc cggaaggcat ggggcttatc gtgcgcaccg
    25141 ctggcgtcgg caaatctgct gaggcactgc aatgggattt aagcttccgt ctgaaacact
    25201 gggaagccat caaaaaagcc gctgaaagcc gcccggcccc gttcctgatt catcaggaga
    25261 gcaacgtaat cgttcgcgca ttccgcgatt acttacgtca ggacatcggc gaaatcctta
    25321 tcgataaccc gaaagtgctc gaactggcac gtcagcatat cgctgcatta ggtcgcccgg
    25381 atttcagcag caaaatcaaa ctgtacaccg gcgagatccc gctgttcagc cactaccaga
    25441 tcgagtcgca gatcgagtcc gccttccagc gtgaagtccg tctgccatcc ggtggttcca
    25501 ttgttatcga cagcaccgaa gcgttaacag ccatcgacat caactccgca cgcgcgaccc
    25561 gtggcggcga tatcgaagaa actgcgttta acaccaacct ggaagctgcc gatgagattg
    25621 ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt tatcgacttc atcgacatga
    25681 cgcctgtacg ccaccagcgt gcggtagaaa accgtctgcg tgaagcggtg cgtcaggacc
    25741 gtgcgcgtat tcaaatcagc catatttctc gctttggcct gctggaaatg tcccgtcagc
    25801 gcctgagccc atcactgggt gaatccagtc atcacgtttg tccgcgttgt tctggtactg
    25861 gcaccgtgcg tgacaacgaa tcgctgtcgc tctctattct gcgtctgatc gaagaagaag
    25921 cgctgaaaga gaacacccag gaagttcacg ccattgttcc tgtgccaatc gcttcttacc
    25981 tgctgaatga aaaacgttct gcggtaaatg ccattgaaac tcgtcaggac ggtgtgcgct
    26041 gtgtaattgt gccaaacgat cagatggaaa ccccgcacta ccacgtgctg cgcgtgcgta
    26101 aaggggaaga aacgccgacc ttaagctaca tgctgccgaa gctgcatgaa gaagcgatgg
    26161 cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc ggaacaacct gcgctggcaa
    26221 cctttgccat gccggatgtg ccgccagcgc caaccccagc tgaacctgcc gcgcctgtcg
    26281 tagccccagc acctaaatct gcaccggcaa caccagccgc tcctgcccaa cctgggctgt
    26341 tgagccgctt cttcggcgca ctgaaagcgc tgttcagcgg tggtgaagaa accaaaccgt
    26401 ccgagcaacc aacaccgaaa gcagaagcga aaccggaacg tcaacaggat cgtcgcaagc
    26461 ctcgtcagaa caaccgccgt gaccgtaatg agcgccgcga cacccgtagt gaacgtactg
    26521 aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg ccaggcacag cagcagactg
    26581 ccgagacgcg tgagagccgt cagcaggctg aggtaacgga aaaagcacgt accaccgacg
    26641 agcagcaagc gccgcgtcgt gaacgtagcc gccgccgtaa cgatgataaa cgtcaggcgc
    26701 aacaagaagc gaaggcgctg aatgttgaag agcaatctgt tcaggaaacc gaacaggaag
    26761 aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca gctcaatcag aaagtgcgtt
    26821 acgagcaaag cgtagccgaa gaagcggtag tcgcaccggt ggttgaagaa actgtcgctg
    26881 ccgaaccaat tgttcaggaa gcgccagctc cacgcacaga actggttaaa gtcccgctgc
    26941 cagtcgtagc gcaaactgca ccagaacagc aagaagagaa caatgctgat aaccgtgaca
    27001 acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca cctgcgcgtg agtggtcagc
    27061 gtcgtcgtcg ctatcgtgac gagcgttatc caacccagtc cccaatgccg ttgaccgtag
    27121 cgtgcgcgtc tccggaactg gcctctggca aagtctggat ccgctatccg attgtacgtc
    27181 cacaagatgt acaggttgaa gagcagcgcg aacaggaaga agtacatgtg cagccgatgg
    27241 tgactgaggt ccctgtcgcc gccgctatcg aaccggttgt tagcgcgcca gttgttgaag
    27301 aagtggccgg tgtcgtagaa gcccccgttc aggttgccga accgcaaccg gaagtggttg
    27361 aaacgacgca tcctgaagtg attgctgccg cggtaactga acagccgcag gtgattaccg
    27421 agtctgatgt tgccgtagcc caggaagttg cagaacaagc agaaccggtg gttgaaccgc
    27481 aggaagagac ggcagatatt gaagaagttg tcgaaactgc tgaggttgta gttgctgaac
    27541 ctgaagttgt tgctcaacct gccgcgccag tagtcgctga agtcgcagca gaagttgaaa
    27601 cggtagctgc ggtcgaacct gaggtcaccg ttgagcataa ccacgctacc gcgccaatga
    27661 cgcgcgctcc ggcaccggaa tatgttccgg aggcaccgcg tcacagtgac tggcagcgcc
    27721 ctacttttgc cttcgaaggt aaaggtgccg caggtggtca tacggcaaca catcatgcct
    27781 ctgccgctcc tgcgcgtccg caacctgttg agtaataatt agctcaaagt aatcaagccc
    27841 tggtaactgc cagggctttt ttatttcatc tttgaatcaa catattaaaa ttcatcgaat
    27901 ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa tgcccgcccc agcgatccca
    27961 gagcaggcag acaaaacata cccagtttca tgatatgttt taaagcgaaa ttatttgttg
    28021 agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt tatacgatgc ctgcaatgcc
    28081 gtttgctgca tgatgtaaga tgaaatagtt gcattccagt ccacatcaac cagatcgctc
    28141 atctgctgcg tttgccctaa agcgcgatcg ctacctaatg aatccagcga ctccagttcg
    28201 ttcagctgcg tgcctaattc cgcgcgaaca gtcagcacat tgttcagcga gtttttcagt
    28261 ccgcggttgg ttttatctaa cgccgcagcg gcggtttctt tatccgcttc gctatccgcg
    28321 accggcgttt tcagggctgc gatggcacta tccagcatgg caaaaagatt ggtttcagaa
    28381 gcgctaccgt ctggttccgc taccgcgttg ctggtaatac tgtcgaaaat tttgtcaccc
    28441 gtgtgcccta tcaccatcga acgcgaagca tcgacctgtt gtttaatact ttctgctcca
    28501 ccgacgtatt tccctttctc ttcgctaaac ggcgcagtct ctgttttata accggcaaaa
    28561 atgtagcgcc cgttaccgtc agtggtgttc gccagattca gcaactggtc acgaagcccc
    28621 tgaatatccg tagccagcga ggcccggtca tcgtcactca aggtgccatt gctggcgtag
    28681 acaatttttt cctgagcatt ctggatagca gtggtgacct ggctaagtac actctcttcc
    28741 agtgacactt tttgagtggc gaaagtacgt gccagcgtgt actggctgtt ttgcgcctgt
    28801 gcctgggaga gaactacggc ttgtgatgct gcaatgggat cgtcagaagg gttaacgact
    28861 cgcttacccg tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga attggtgatg
    28921 ccgcgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc tccccttagc
    28981 gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc gcattcgcca
    29041 ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc atcgagattg acaccggaaa
    29101 tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg ctactggttt
    29161 tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg tcgttaaagg
    29221 atttcgcacc gcccaccgtt ttactgttgc tttgcagatc cagcagggcc tgaccgttgc
    29281 ggttatcgct atcacccgca tctttttcgc tcgccatcgc tattttcgct tcgtcggtga
    29341 ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag ctgtcgttaa
    29401 cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta ccgttggcat
    29461 ccggcgtcac cgtaaaagtg gtattgctgg caaggcgggt gacctgccac tgattattat
    29521 cgaacgagat tttgtaatct gtcgccagta ccgcggaggc atcagttacc gtggcaccga
    29581 tcgcaacgtc acctttgttt ttcgtgtttt gcagaaccgc gggcttaccg atagcaaaga
    29641 aatcttcacc agcatcgccg ttggcatcaa atccggcttt gtgttggctg ttgaaagcct
    29701 cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt ctggtccaga tcctgagaac
    29761 ggaatgtcag aatgccgccc agcgacccgg tattcagtaa tttctccggg atctcaatat
    29821 tgcctgccgt cccatcaacg taagcgacag tcgtacgaga agggtcggcg ctggaaggaa
    29881 ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg gccatcgtga
    29941 tgttataagt accgccatcc tgaacgctga cttctacgcc aacaatctgg tttaattcgc
    30001 tcaccagttg atcgcgttga tccagcagat tgttaggtga cgcccctgcc cccacgcctg
    30061 tcaggcgcga gatttgatcg ttcaggctgg caatttgttt agcgtaattg ttgatctgat
    30121 caacgctggc acctatcgcg atattgacct gtttgtcctg gtcgcgcaga tattgatcgg
    30181 tggttttaaa ctgattcacc aacccttctg atttcccaat cagcgcctgg cgcgctgccg
    30241 ggtcttccgc gttactcacc agcgtttgca ggctggtgaa gaaatcctgc atctgtgttg
    30301 ccagcgaaga ggtactggtg gagagcatat tgtcgatttt cgacatctgc tcatagcggg
    30361 cggtcaggcc gctactttgc gtctgcgcag cacgtaactg gttggtaata aacgcatcat
    30421 actcacgctg cacaccagaa acgtagacgc cattgccaac ccagccgcca gcgcccaacg
    30481 tgctattggc ctgcgccata atagtggttt ggcgggtata tccggcaacg ttatagctgg
    30541 agatattatt acttgccgta tttaacgccg cctgggccgc gttcagtccg ctcatggcgt
    30601 tattgatcaa gctggacatg gaggttcctt ataagccttc aattacagag tattatcggc
    30661 agcgacccgc cggacttgag ttattcagaa cagattatca atgttcatgc tgtaggtttt
    30721 gctcaccttg tcgcttatcg atttcatctg ctggatcatg ttggtgagtt tacgggcata
    30781 gtgaggatcg gtagcatacc ccgcgtcctg tagggcctgc gccccctgct ccgcactcgc
    30841 ggcggtcgtc acggcggcgt agcgcgggtt acgtgttaac agcccaacgt aatccgacaa
    30901 tgcttccaga tacgagctgt agacccgaaa cttcgctttt actttcttcg cttcgccgtt
    30961 ttcatattca gtcgtggtga tttcagtaac tggccctttc cagttgccag aggctttgac
    31021 accaaacagg ttatagctcg gctcgccgtt ttcgcggcgg atttgccgtt gcccccaacc
    31081 agattccagt gccgcctgag cgaggatcaa atgatgtggc acaccgcttt gctggcttgc
    31141 cagttgggcg ggcagcgaga gttgcgcgag gaatgcttta ctgtcacccg gcagcgaatc
    31201 atcgtagtta cgtggcacgg ccttttgcac cagctgcgaa agcgcctgat tttgataacg
    31261 caccacagtt tcgagcggga atttcatcgg tgctgctggc gtggactcct ctggcaatgg
    31321 ttgttctggc gtcatctgtt taaccatcat ctctgcaagc cccagacctt tgcccgccgt
    31381 catctgttgg gcaatctgct ggtcatacat actggtatac aggcgagtgt gctcgctgct
    31441 gaacaggcca tcttttggta aagcgtcgcg catgcttttc aacatcatct gcacgaacat
    31501 cccttccacc tgacgggcca ccggacggat atttgccgcc ggatcttcgc ccgctttcgc
    31561 ctttagttcg ttgagtgatt gcgcatccca ggccgcactt gccagtagtt tgctgtcgct
    31621 gatcatcaga tgatttccag ttttgcccgc agacatcccg cactttgcat tgattgcagt
    31681 atggacatca gatccatcgg cgtagcgccc agcgcattga gcgcgcgcac cacgttattg
    31741 aggctggcgc tggaacgtac gctttgcagc gaaccgccgc tctggcgtaa atcgatctgc
    31801 gtttgtggag taaccacagt ctgtccacca ccaaacggtg tatctggctg gctgacattg
    31861 gcctgacgat taactgttac tgagagattc ccctgcgcta ccgcgcagct gtcgagggtc
    31921 acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa tcactacttt agcgtcctgc
    31981 ggggtgacat taacatgcat attctgaata tcggcaagga agcggacctg ggaactgttg
    32041 ccactcggta cgcgcacctg aatagtccgc gcatctaacg cggtggcgct gccatatcca
    32101 cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc tgaaatcttc gtcgttaagt
    32161 tgcaaattaa gggtattccc gacgccaaac tggctgggca attcacgttc aataaccgca
    32221 ccattggtga tccgtccacc gttcagttgg ttaacctgaa cactgctacc gccagcggag
    32281 gctcctgcgc cgccaaccag aatattgccc tgcgccagcg catacacctg actgtcaacg
    32341 cccttaagcg gtgtcatcaa caacgtacct ccacgcaagc ttttggcatt acccatggaa
    32401 gaaaccacca catcgatggt ttgcccctga cgtccaaacg gaggaagtga cgctgtcacc
    32461 attaccgcag cgacgttttt tagctgcata ttggtgcccg tcggaacggt aattcccagc
    32521 tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg tctgggttgt ctggtcaccg
    32581 gtgccatcca gccccaccac cagaccatag ccaatcagtg agttttgcct taccccctga
    32641 acactggtga gatcgcgaat acgctcagcc tgagccgccg tcgtgaccag tagaagaatt
    32701 aatgcagaga gaaatttaat caccacagcc tcacttacat tggcgacagg ttaaggaaga
    32761 aacgctgcaa ccagcccata ttttgcgctt cgttaatgta gccattgcct acgtattcaa
    32821 tgcgcgcatc cgccacctga gtagacggta cggtattgct gccgctgata gtgcgtggat
    32881 taaccacacc agagaagcga ataaattcgg taccctgatt aatggcaatc tgtttttcac
    32941 ccaccacatg caggttgccg ttgaccagta cctggtcaac cgtcaccgtc aacgtgccgc
    33001 taaaggtatt gctggcattg gccccgccct ttccgttgaa cgtgttacca ccggaggctt
    33061 cgacatcggc acgagcgtta ccaaacagcc cctgcaaata gcgcggcaca gtatcaaagc
    33121 caaaattagt tttaccgtca cggctggcat tcgcagagga gcttttgctg gcgctgacgt
    33181 tctcctgcaa cacgatggtc agcgtatcgc caatattgcg tggtcgacga tcttcaaaca
    33241 gcggttgata gccatagtta atcggctgag cagactggaa aatagaaccg ttggcgacgg
    33301 gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg caccagcggc gtggagggta
    33361 tccaggcgca gccggttagt gaaagcacca acaagctgga aatagcataa gtatgcgcag
    33421 cgtttttttg catggctatc atcttcaaaa atcagtaaac cggtgaacct gccaccggtt
    33481 atgccttaga gttgcgtcag tttttgcagc atctgatcgg tggtggacac cgctttactg
    33541 ttgatttcgt aagcgcgttg cacctgaatc atattgacca gttcttccgc cacgttgacg
    33601 ttagacgttt caacataccc ttgatacagc agtcccgcgc cgttcaggcc cggcgtgctt
    33661 tcgttcggtg caccggagga ttgcgtttcg gtgtagaggt tttcgccaat gctctccagc
    33721 ccggtatcat tcataaaggt ggtgagattg agctgcccaa cctgaaccgg agctgcctgg
    33781 ccttgttggg ttacgctgac cacgccatca cgaccgatgg tgatacttaa cgcattcgcc
    33841 ggaatggtga tcgctggctg cacctgaaaa ccaccagccg tcaccagctg cccgttctga
    33901 tccacctgga aagagccgtc acgggtatag gctgatgagc catctggcaa catcacctgg
    33961 aaaaagccct gccctttaat cgcgacatct ttgctgttgt tggtctgcga caggttgccc
    34021 tggctgtgta aacgttcagt ggcgaccggg cgtacccccg tgccgatttg taatccggag
    34081 ggtaaggtgg tttgttcgga agactgtgcc cccggctggc gaatggtttg ataaagcaga
    34141 tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac tgacgtttgc caggttgttg
    34201 gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg ttttggcgat ccataatgaa
    34261 ctgatcataa aatgtcctgt agtggattag tgacaaagtg catatgtctt cttgccggat
    34321 gcggcgtaaa cgccttatcc ggcctacaag agctcgcaaa atcgataaat tgcgattgcc
    34381 cccatagacc cgataaactt gcgcatcggg caacttatac ttgtcatgta tcctttcaat
    34441 taactcatcg acagcagttg gttggcacgg cctgcgttat catcgacgct gctgatcacc
    34501 ttcatctgca tttcaaaacg ccgcgcgctg gcaatcatgt cgctcattgc cgcaacggca
    34561 ttgacgttac tgccttccag aacccccgac atcacacgca aggttggatc tgcctgcagt
    34621 accggcccac gcgtggcctg ggtttctgcg cttaaacgaa aaatgccgtc gtcaccgcgc
    34681 tgcacttcgc tgcccgtggc tttcaccagt ttaagacgcc ctactggcgc aaccgtattt
    34741 gccggatcgc ccggattgag cgccgagatt gtgccatcgg cagcaatagt gatttccgcc
    34801 ccttccggca cagcaattgg cccagcctcg cctatcaccg gatgcccctg aattgtcagt
    34861 tgcccggtgg gatcaacctg aatgctgcca ttacgcgtat acccttcgct gccgtcagcg
    34921 gactgcacgg ccagccagcc atcctgctgc aacgcaacgt ccagcgggcg cgaggtgtaa
    34981 tccatcttgc cgggcgtcat gtctgcaccc ggtgttgacg ccgttaccag cgtgcgcgtg
    35041 ggcagtgaaa gcccttccac cggcactgca cgcaaggcgt ttaactgcgc gcgaaaaccg
    35101 ggcgtagagg cattggccag attactggcg gttaccgcct gttgattcag cgtctggctg
    35161 gctgctccca tcgcggtata aattgcgtga tccattgagc tatcccgtca gcgattagcg
    35221 taagttaacc agcgtgttga ggatctggtc ctgggttttg atggtctggg cgttagactg
    35281 atagttacgc tgggcaacga tcatattgac cagttcttta ctgagatcga cgttggacgc
    35341 ttccaacgca ccgttggtca gggtgccaaa gttgcccgtc ccggctgacc ccaacagcgc
    35401 cacgccagaa gattgcgtcg cagaccagac gttgtcgcct tcggatgcca gaccttcgtt
    35461 gttggcaaag ttcgccagta caatctgccc cagcagttgt gtttgttcgt tggaatagtt
    35521 gccgacaacc gtaccgtcat cattgatttg ataactcacc agatcgcccg gtttgtagcc
    35581 attctgggtg gttgccacaa tattgttagc gccggtattt tgctgcatgg agttgaggaa
    35641 gctcaggcta aacgtggcgg gatctgcgcc gttaattgcg ccagtggtaa tattctctgt
    35701 tggatttgag gtcagaacgc cattggcatt aaacaccagc ttcattgcag gctcggctgt
    35761 acctgttgga tcactgctat cctgggtgta gacctgccag ttattatccc cggtcttcac
    35821 aaagtagacg ctcatgtcat gagcattacc ctgactgtcg aaaacagtca ccgaaccttt
    35881 tttgttatag ctatccgcat tgctggcgct gaatggcgta acagtaggaa gcggatcgct
    35941 ggaattcagg ttgatctgca tcgatgccgt ggtggtagtt ttcgctgcca tcagggtatt
    36001 cgggatcgaa atattagtcg gattcgcccc ttgctgaata gtcggcggcg taccggttgc
    36061 cgggtaaccc gtcagctgta aaccttgcat attcaccagg ttacggtttt catccagctt
    36121 aaattgtccg ttacggctgt agaacaccga accgttgctg tctaccagac ggaaaaaacc
    36181 gttctggctg atagcaacgt ccagacctcg cccggtgttg gtggtcgtgc catcggtaaa
    36241 gtcctgagtg ataccggcaa cttttacccc cagtcccact ttcgaaccgg caaacatatc
    36301 ggcaaaagag gccgtgcctg atttaaagcc gtaggtggcg gagttggcga tattgttgcc
    36361 aataacatcg aggttggtgg cggcagcgtt taatccgcta accgcttgag aaaaggccat
    36421 gactgactcc tgaaatgtga aggcttaaat tatctgccgt acttcgtcga gggtggtggt
    36481 gccgtaagtg ccgagatcca gcgtattacc gttgttgccg cggatcacac cctgcaccag
    36541 agcaaactgc agcggctggg caaccagttg tgtaccaccg ttactggcgc taatcgctac
    36601 attgtaagaa ccgttcggcg cagtgctgcc atcagtcaac gtaccgtccc aggtgaaact
    36661 gtgaactccg gcggtcagtt caccaatatc aatggtgcgc acaaccgcgc catttttatc
    36721 ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt
    36781 cgtggtcaca gccccttctt cactgccggt tcccgcaaga atagtggtgc cggggatcat
    36841 cacgccgtga ccgatcaggt tactggcctg taacgactgg ctgttatcaa tctgtccgga
    36901 aatagatccg agcgtggtat tgagtttttc aatcccgctg accgtgctga tttgtgccag
    36961 ttgcgacgtc agctcgttgt tttccattgg attggtcggg tcctggtttt tcagctgcgc
    37021 caccagcaaa gtcagaaaac tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact
    37081 actgctggtg gtactgacgc cggtatttgt cggatcggtg gtggttaccg caatggacat
    37141 agctttctcc tttattgacc gagcgtaagg gttttcagca tcatgctttt caccgtgttg
    37201 agcacttcaa cattggcctg atagctgcgt gacgccgaca tggtgttaac catctccccg
    37261 acgacatcaa cgttcggcat ttttacgtag ccctttgcat ctgccagcgg attacccggt
    37321 tcataaacca gtttgtccgg ggcctgactt tctataacat cggcaacctt tacgccgcct
    37381 gtcgcagcac ctggtgcagc gttaacctgg aataccacct gttttgcccg atatggctgt
    37441 ccatcaggac cggtcacgct atcagcattt gccagattac tggccgccac gttcaggcgc
    37501 tgggactggg cagttaacgc cgacccggcg atatcaaaaa tattcagcag tgccatccgt
    37561 taatttccgc tctgtaaaac gttcatcatg cctttgattt gcccgctcaa cgcgctaagg
    37621 ctcatctggt attgcaggct gttatcggca aactgggtgc gttcgcgatc catatcgacg
    37681 gtattaccgt caagcgaagg ctggtccgga atacggtatt gcagttctgc ggtaggaggc
    37741 gtcagcgcct gcgccgggat gtgttgcgtt gaggtcatcg tcagtgcaac cacactggtt
    37801 gcatcccgtc cacgttgcat gactttttta agttcactgg caaaatcgat atcgcgcgcc
    37861 tgataaccag gggtatcggc attggcgatg tttgctgcca gcacttcctg acgctgggcg
    37921 cgcagattga gcgcctcttg ttgaaaacgt aaggcggcgt cgagcttatc gagcatatct
    37981 cctccgcagg tatcaaaatt ctgccatcag cttaaatgcc tttacgcgcg cgttatcggc
    38041 ggaataaacg caaaatgggt cgctatttat gccgttgatg accattgcgg acaggtacaa
    38101 ttcacgttgt agaaatggct ggggggcgaa aatgctggca ataaaacgta gcgtggcgat
    38161 catcgcgata ctgttcagtc cgttaagtac ggcgagcaat ctcacatcgc aattgcacaa
    38221 cttttttagc gcccaactcg cgggggtaag tgatgaggtt cgtgtttcta ttcgtacagc
    38281 gcccaatcta ctaccgccat gcgagcagcc attgctttcg atgagcaata attcccgcct
    38341 gtggggcaat gtgaatgtgt tggcacgctg cggtaacgac aaacgatatt tacaggttaa
    38401 tgtgcaggcc acaggaaatt atgtggttgc cgcaatgccc attgtgcggg gaggaaagct
    38461 ggaagctggc aatgtcaaac taaaacgcgg acggctggat accctgccac cgcgtacggt
    38521 gctggatatc aatcaacttg ttgatgccgt tagcctgcgc gatctatcac ccgatcaacc
    38581 tatccagtta acccagtttc gccaggcatg gcgggtaaaa gcgggacaac gcgtcaacgt
    38641 gatcgccagc ggtgatgggt ttagcgccaa cgcagaaggt caggcgctga acaatgcagc
    38701 cgtcgcacag aatgcgcggg tgcgcatggt atcgggacag gtagtcagcg gcgttgttga
    38761 tgcagatggg aatattctta taaacctgta agctgttaaa gattacccgt cccttgccga
    38821 taaataagca acacatgata aaagcgccct caatgaggaa taaaccatga gtattgatcg
    38881 cacttcgcct ctgaagcctg taagcaccgt tcaaccgcgc gaaaccactg acgcgccggt
    38941 aacgaacagt cgggcggcaa aaacaaccgc ctccaccagc accagtgtga cgttaagcga
    39001 cgcgcaagca aaactgatgc aacccggcag cagtgatatc aatcttgaac gtgtcgaagc
    39061 gttaaaactg gcgattcgta acggtgaact aaaaatggac accggcaaaa ttgccgatgc
    39121 gctgatcaac gaagcgcagc aagacttgca gagtaactga tcgtatgaca cgtcttgcag
    39181 agatcctcga ccagatgtcc gctgtgctta acgatctcaa aacggtaatg gatcaagagc
    39241 agcaacatct ctctatgggg cagatcaacg gcagccagtt gcaatggatt acagaacaaa
    39301 aaagctcact gctggcgacg ctggattacc tcgaacagtt acgcaagaaa gaacccaata
    39361 cagcaaatag cgttgatatt agtcaacgct ggcaggaaat tactgtgaaa acgcagcaac
    39421 tacgccaaat gaatcaacat aacggctggt tactggaagg acagattgag cgcaatcaac
    39481 aggcgctgga aatgttgaaa ccgcatcagg aaccgacgct atatggggcg aacggtcaga
    39541 cctcaacaac ccatcgcggc ggtaaaaaga tttcgatctg aagattatct ccggcctgca
    39601 ctgcaggccg gaatgcattg ttacaccgtc cggcgggcaa attctttaac tttgaagccc
    39661 agtaccgcca gtgcagcgaa gtacgcggca atccccgcca gcacgaccgc cattaaacgc
    39721 agtaagcgcc agggcatggt acccaatgac cactccggca tgatatgtaa catacctaaa
    39781 agcacgccag acatcaccag taccgccacc accagacgca acagaaacgc catccagcca
    39841 ggttgcggag taaagatttt ctgcttacgc aactgccagt aaagcagcga agcattcaga
    39901 cacgccgcca gaccaataga aagtgacagc ccggcatgtt tcaacggacc aataaacgcc
    39961 aggttcatca attgcgttaa aatcagcgta acgatggcaa ttttcactgg cgttttaatg
    40021 tcctggcggg aataaaagcc aggagccaac actttcacta caatcaggcc aatcaaaccc
    40081 accgagtagg caattaacgc ccgctgggtc atcagcgcat caaacgcggt aaatttaccg
    40141 tactggaaca gcgaaacggt taacggacct gaaagaatgc ccaacgcaac cgcactcggc
    40201 agcgccaaca ggaaacaaag acgcaacccc cagtccatca aacggttgta ttcatcatga
    40261 ttgccactgg caaaactttt cgacagcgat ggcagcaaaa tggtgccaag cgccacgccc
    40321 agcacaccgg agggaaactc cattaagcgg tcggcgtaat acatccaaga caccgaaccg
    40381 gaagcaagaa acgaggcaaa aatggtgttg atgattaagg agatctggct cacagagacg
    40441 ccaaggatcg ccggtcccat ctgtttcatc acgcgcattg ctccggcatc gtggaagtta
    40501 atgcgcggca ggaccagcat gccgatcttc ttcaggtgcg gtagctgata caccagctgc
    40561 aggatgccgc cgaccgtaac agcccacgcc agcgccagca ccggtgggtt aaagtacggt
    40621 gcggcaaaca gcgcgaaacc aatcatgctg atgttaagca gtgttggcgc aaacgccgga
    40681 atcgagaagc ggttccacgt attcagaatc gctcccacca gcgacgccag ggagataagc
    40741 aagatatagg gaaaggtaat ctttagtagc tggctggtca gggcaaattt gtcggcagtg
    40801 tcagcgaagc ctggcgcggt caccatgatc acccacggtg cggcgagcat gccagcgacc
    40861 gtcacaaccg ccagcgcgag tgtcagcagg ccagaaacat aagagacaaa gacccgcgtg
    40921 gcgtcttcac cctgcttact tttatattcc gccagaatcg gtacaaatgc ctgggaaaat
    40981 gccccttcgg caaagatacg gcgtaacaag ttaggaagtt taaaagcgac gaaaaaggcg
    41041 tcggttgcca tccctgcgcc aaagattctg gcgacaattg cgtctcgtgc gaagccaagc
    41101 acacgcgaaa acatggtcat cgagctgacg gcggccagcg attttaataa attcatcggt
    41161 gttctaatcc agacccacgg gcaaaacgcc tgcaaggcag gcgctgaaag aggcgaatag
    41221 tctacctgga ttatggtgaa ttgctaccgc cagatgttac agggtttatt cactcatcgc
    41281 atcgcgccag atcttgtcaa cgatacgttg cgccagcacg gcctgttcgc cggcggtttg
    41341 cggaactgtc tgattttgca cacattcaat gaagtgccgt gcacagccga caaatccacg
    41401 ttgctcaagc gtactctgcc aaccaggaat cggtttatgc actacgccct gcccgcgctc
    41461 ctcacgccat tcgcgcatat ccgtaatgtc gatgagcgca ccgtcagtca cggcctgcac
    41521 ggtttcacgc tgacttccgg cacggcgatg catacaggtg gtgatctgca aagggccggc
    41581 cgaaaaatgg tgctcggcaa acagcatttc gccagcgtcg ttagtcaata gcgtaccgcc
    41641 atccagagaa gctttgccgc ccgacaacca cagcgcggta tccaccacat gcagataatc
    41701 atccagcaac gtgaaataaa gatcgtgtgg cccgacgcta ttgctacgat gtttatccat
    41761 tcttagcgag gctgcggtag cgagttgcgt ttttaactca ccgtagagtg gtgcgaaacg
    41821 acggttaaaa ccgaccatca acgtcagttt tttccgcgcc gccagttcca ccagccgttc
    41881 agcatcgcgc agattttctg ccagcggttt atcgacacag acatgtaccc ccgcattgag
    41941 taacgtactg accacgtcaa agtggctggc ggtgctggaa tgcacaaaaa ccgcatcgca
    42001 actggcggca aggctggata acgaatcggc atatggaatg cgccagcttt cacaaattgg
    42061 cagggctttc gcgcgcgtag gcgaccaggc tccttgtaac gtccagtcag acgctgccgc
    42121 cagcaccggt aaccacgctt tttgcgcaat gccacctaat cccactacgc cgatacgtaa
    42181 ttttttcact ttaatctccc agatgggcca gcaacgaatc aagacgctgt ttcagttctg
    42241 ccacttcgat ttccagggct tcgacgcggg cctgtaaatc accatcaacc gcgttcgaca
    42301 tatccgtcac cgccggctga tcttcaacct caccactgaa aagatgcatg tagcggcttt
    42361 cgcgtttacc cggttcgcgg gccagacgca ccacaaaagg accatcttcg cgatttgcca
    42421 gttgttccag cgtcgactcc acttccgcca tatcgctgaa ttcatacatt cgcgcggcgc
    42481 ggctgcgcag ttcacctggc gtctgggcac cacgcaataa caacgtggtg attaacgcca
    42541 cttccgctgc gctcagtttc agatcgccaa attctgaatt acaaaaacgt tgctcatatt
    42601 tggtgacccg attaccaaaa ccgctcactg tgcgtagata atgacgtttg accagattat
    42661 ccagctgttc ctgcacttcg gattcactca gattcatgac cggttcacgg ttcgttttct
    42721 gattacaggc cgtgactaca ccattgactg agagcgggta ttgctccggc gtggtcacct
    42781 gcttttccag caagcaacca atcacgcgag cttcaagggc agtaagttga tatttcatga
    42841 gattctcctt agcggccggg cgtccagtct ggggtagtta atgccgtcag tacgtgatcg
    42901 cgccattgtc catcaatcaa cagatagtct ttcgcatagc cttctttttc aaaacccagt
    42961 cgcgccagta aatcaccgct gcgtttattg tgcggcatat aattagccat aatgcgatga
    43021 atatgttggg tgcgctgcat ataacgaatg gctgcggtca gggcttcaaa catgagtcct
    43081 ttgccctgcc atttttgccc aatcgaataa ccgagatagc aggcatgaaa agagccacga
    43141 acaacattgg aaaaattggc aacgccaata atctcttttt cgtccgggtc gaataagcca
    43201 aagtagaaag ctgaaccttg tttatgaaat tcgttaatca tccccagcct ggcctgccag
    43261 cctgatggat aacagtggct ttcgtcgcgc actggctccc agggcttgag gaaatggcga
    43321 ttctctgcgt aataatccgc aagacgccag gcatcacgat catgcaccag acgcacgacc
    43381 agtcggtctg tggttaagcg cacttttggc acgttactgc gatagccaaa cattctatac
    43441 ctactccttc ccgtaacgtc tttaatacgc tgtaagtatt actatacctg cgcaaccaga
    43501 ccctgtgaaa agtccaaaat gccattttct gcattattca catatttgat aaaaactctc
    43561 tatcaaagcg acgttttgat aaaaaaatat tgtccccgaa gtgatgggaa aagtcttagc
    43621 ggcaatgcag aatagaaagt gctcatcttt tatttcccgg gaggggaaat gtcccgcgtg
    43681 tcgcaggcga ggaacctggg taaatatttc ctgctcatcg ataatatgct ggtcgtgctg
    43741 gggttctttg ttgtcttccc gctgatctct atccgcttcg ttgatcaaat gggctgggcc
    43801 gccgtcatgg tcggtattgc tctcggtcta cgccaattta ttcagcaagg tctgggtatt
    43861 ttcggcggcg caattgccga ccgctttggt gccaaaccga tgattgttac cggtatgctg
    43921 atgcgtgccg ccggattcgc cacaatgggt atcgcccacg aaccgtggct attgtggttt
    43981 tcatgcctgc tttcgggact cggcggcacg ttgtttgatc cgccgcgttc ggcgctggtg
    44041 gtgaaactaa tccgtccaca acaacgtggt cgttttttct cgctgttgat gatgcaggac
    44101 agtgccggtg cggtcattgg cgcattgttg gggagctggc tgttgcaata cgactttcgc
    44161 ctggtctgcg ccacaggggc cgttttgttt gtactgtgtg cagcgttcaa tgcgtggtta
    44221 ttaccggcat ggaaactctc caccgtacgc acgcccgttc gcgaaggcat gacccgcgtg
    44281 atgcgcgaca agcgttttgt cacctatgtc ctgacgctgg cgggttacta catgctggct
    44341 gttcaagtga tgctgatgct gccaattatg gtcaacgacg tggcgggcgc gccctctgcc
    44401 gttaaatgga tgtatgccat tgaagcgtgt ctgtcgttaa cgttgctcta tcctatcgcc
    44461 cgctggagtg aaaagcattt tcgtctggaa caccggttga tggctgggct gttgataatg
    44521 tcattaagca tgatgccggt gggcatggtc agcggcctgc aacaactttt caccctgatt
    44581 tgtctgtttt atatcgggtc gatcattgcc gagcctgcgc gtgaaacctt aagtgcttcg
    44641 ctggcggacg caagagctcg cggcagctat atggggttta gccgtctggg tctggcgatt
    44701 ggtggcgcta ttggttatat cggtggcggc tggctgtttg acctgggcaa atcggcgcac
    44761 cagccagagc ttccgtggat gatgctggga attattggca tcttcacttt ccttgcgctg
    44821 ggttggcagt ttagtcaaaa acgcgccgcg cgtcgtttgc tggaacgcga cgcctgattt
    44881 gatactcccc cctgactcct ccatactgaa agtgaactga cagccaaatg gaggagtcac
    44941 gtgaagctat acatttacga tcactgccct tactgcctca aagcccgcat gattttcggc
    45001 ctgaaaaata tccccgtcga attacatgtt ctgctcaacg acgacgcaga aacacccacc
    45061 cggatggtcg gtcaaaaaca ggttcccatt ctgcaaaaag atgacagccg ctatatgcca
    45121 gaaagcatgg atatcgttca ctatgtcgat aaactcgacg gcaaaccgtt actgaccggc
    45181 aaacgttccc ctgccattga agagtggctg cgcaaagtca atggctacgc caacaaactg
    45241 ctgttgccgc gttttgccaa atcggcattt gatgagtttt ctactcccgc cgcgcgcaaa
    45301 tatttcgtcg acaagaaaga ggccagcgcg ggtaattttg ccgacctgct ggcccactct
    45361 gacggtctga ttaagaatat cagcgatgat ttgcgtgcgc tggacaaact gatcgtcaaa
    45421 ccgaacgccg tgaatggcga actttcggaa gatgatattc agctatttcc gctactgcgt
    45481 aatctgacgc tggtagccgg aatcaactgg ccaagccgcg ttgctgatta ccgcgataat
    45541 atggcgaaac agacacaaat caatttgtta tcatcaatgg cgatttaatc ctgacccgcc
    45601 gcggcagcct gtattcgcgg gttgccgccg ttttcagttt tttctgctgg cgagtttatc
    45661 gctggtgaca catgctgcgt tgtgtcagga tattgacgca gatttcatgt cgaggactac
    45721 catgaacaag tttttatttg ccgctgcatt gatcgtcagt ggcctgctcg ttggctgtaa
    45781 tcaactcacc caatacacca tcaccgaaca agaaattaac cagtcgcttg cgaaacataa
    45841 taatttctca aaagatatcg gtttacccgg cgtggctgac gcccatattg ttctgacaaa
    45901 cttgaccagc caaattggtc gcgaagagcc gaataaggtt accctaaccg gagacgccaa
    45961 tctggacatg aactccctgt tcggtagtca gaaagcgacc atgaaactga agctgaaagc
    46021 gctgcccgtg tttgataaag aaaaaggtgc gatcttcctg aaagagatgg aagtggtcga
    46081 tgcgacggta caaccggaaa aaatgcaaac ggtgatgcaa acgttgcttc cctatttgaa
    46141 ccaggcatta cgcaattact ttaaccagca acctgcttac gtcctgcgcg aagatggcag
    46201 ccagggcgaa gcaatggcga aaaaactggc gaaaggcatt gaagtgaagc caggcgaaat
    46261 tgtcattcca tttactgatt aatcacgagg gcgcatttgc gccctttatt tttcgtgcaa
    46321 aggaaaacgt ttccgcttat cctttgtgtc cggcaaaaac atcccttcag ccggagcata
    46381 gagattaatg actgcaccat cccaggtatt aaagatccgc cgcccagacg actggcacct
    46441 tcacctccgc gatggcgaca tgttaaaaac tgtcgtgccg tataccagcg aaatttatgg
    46501 acgggctatc gtaatgccca atctggctcc gcccgtgacc accgttgagg ctgccgtggc
    46561 gtatcgccaa cgtattcttg acgccgtacc tgccgggcac gacttcaccc cattgatgac
    46621 ctgttattta acagattcgc tggatcctaa tgagctggag cgcggattta acgaaggcgt
    46681 gttcaccgct gcaaaacttt acccggcaaa cgcaaccact aactccagcc acggcgtgac
    46741 gtcaattgac gcaatcatgc cggtacttga gcgcatggaa aaaatcggta tgccgctact
    46801 ggtgcatggt gaagtgacac atgcagatat cgacattttt gatcgtgaag cgcgctttat
    46861 agaaagcgtg atggaaccgc tgcgccagcg cctgactgca ctgaaagtcg tttttgaaca
    46921 catcaccacc aaagacgcag ccgactatgt ccgtgacgga aatgaacgac tggctgctac
    46981 catcactccg caacatctga tgtttaaccg caaccatatg ctggttggtg gcgtgcgtcc
    47041 gcacctgtat tgtctaccca tcctcaaacg caatattcac caacaggcac tgcgtgaact
    47101 ggtcgccagc ggttttaatc gagtattcct cggtacggat tctgcgccac atgcacgtca
    47161 tcgcaaagag agcacctgcg gctgcgcggg ctgcttcaac gccccaaccg cgctgggaag
    47221 ttacgccacc gtctttgaag agatgaatgc tctgcagcac tttgaagcat tctgttctgt
    47281 aaacggcccg cagttctatg ggttaccggt caacgacaca ttcatcgaac tggtacgtga
    47341 agagcaacag gttgctgaaa gcatcgcact gactgatgac accctggtgc cattcctcgc
    47401 cggggaaacg gtacgctggt ccgttaaaca ataaaaatcg tagcttcctg ttgtcattag
    47461 gttattttac ctgtataaat aaccagtata ttcaacaggg ggctattatg cgaattgaag
    47521 tcaccatagc gaaaacttct ccattgccag ctggggctat tgacgccctg gctggcgaac
    47581 tttcccgccg tattcagtat gcgtttcctg ataatgaagg ccacgtatca gtacgttatg
    47641 ccgcagcgaa taatttatcg gttattggcg cgacaaaaga agataaacag cgcattagcg
    47701 aaattctcca ggaaacgtgg gaaagcgccg atgactggtt tgtcagcgaa taatatgcag
    47761 tgatttttgt ttttgccggg tcgccccggc ttttttattt tacttacgaa ttttgagtac
    47821 aaaatttcta cacttcctca aaataattgc acaatatgct aaaaaaatat gaatgacatg
    47881 ctgttttatt gttcatataa atcttaaaag cagaaattta tctgttcccc cctttaaaaa
    47941 ttcggtgaat acccttactt attggtatac tgaagttgct tcagataaaa acgcattgaa
    48001 cctcgaataa cgttgtctag taacacgaat tagggggcca tgatggaaaa aaataatgaa
    48061 gtcattcaga ctcatccgct cgtagggtgg gacatcagca ccgttgatag ctatgatgcg
    48121 ctgatgttgc gtttgcacta ccagacccca aataagtccg agcaggaagg gactgaagtt
    48181 ggtcagacgc tctggttaac cactgatgtt gccagacaat ttatttcgat attagaagca
    48241 ggaatcgcca aaattgaatc cggtgatttc ccggtaaacg agtatcggcg tcattaacat
    48301 gacatgacca tccacccaaa aggcacctca ccggtgcctt taccatttct gggttgtata
    48361 tctgcattca gttaattact ctgcttaaac catgagagca gagagaaaaa aatgaaatac
    48421 gatctcatca ttattggcag cggttccgta ggcgctgccg ccgggtatta tgcaacccgc
    48481 gccggtttaa acgtgctaat gaccgacgcc catatgccac cgcatcaaca cggcagccac
    48541 cacggcgata cgcgattaat tcgccatgct tatggtgaag gcgaaaagta tgtcccactg
    48601 gtactccgcg cgcaaacact gtgggatgag ctctcccgcc acaacgaaga tgatcccatt
    48661 tttgtacgct ctggtgtcat taacctcggc ccggctgact ccgcatttct cgccaacgtc
    48721 gcccacagcg ccgaacagtg gcaactcaac gtcgaaaagc tcgacgcgca agggattatg
    48781 gcccgctggc cagaaatacg cgtcccggac aactacatcg gcttatttga gactgattcc
    48841 ggttttttgc gcagcgaact ggcgattaaa acctggatcc aactggcgaa ggaagcgggc
    48901 tgtgcgcagc tgttcaactg cccggtcacc gcaattcgtc atgacgatga tggcgtaact
    48961 attgaaacgg ctgacggaga gtatcaggcg aaaaaagcga ttgtctgcgc gggaacatgg
    49021 gtaaaagacc tgctcccgga gctgcctgtc cagcccgtac gcaaagtatt tgcctggtat
    49081 caggccgatg gtcgctatag cgtgaagaat aaattcccgg cgtttaccgg tgaactgccc
    49141 aatggcgatc aatattatgg ttttccggca gaaaacgacg cgttgaagat tggcaaacat
    49201 aacggaggcc aggttatcca ttcagcggat gaacgtgttc cgtttgcgga agtggtcagc
    49261 gatggttcgg aagccttccc gttcttgcgc aatgtcttgc cgggtatcgg ttgctgcctg
    49321 tacggcgctg cctgcaccta tgataattcg cctgacgaag attttattat cgatacccta
    49381 cccggtcacg ataatacact gctcattacc ggcctgagcg ggcacggttt taaatttgcg
    49441 tctgttttag gggaaatagc tgccgatttt gcgcaagaca aaaaaagcga ttttgattta
    49501 acgccattca ggctttcccg cttccaataa tcatcattac ggcctccaga atgaggccgt
    49561 ttttacagga gtatttaatg cgtcggctgt tgcactatct catcaataat attcgcgagc
    49621 atctgatgct ttatcttttt ctctggggat tgttggcaat tatggatctg atctatgtgt
    49681 tttattttta gttttccgca ctgacataaa ctgacaaata tttaatctcc ctttcattta
    49741 gcgtgaaaaa ttgaatgaga ttattcctac ttctacagca ctcaccagac atctctgatg
    49801 ctgaacccac taaacttatg aagattcttt cattattaat tcaaaattat tgaaataaaa
    49861 ataaatatca ttcaatttgt tatttttgtg ttgcaattca ttctccgaca cgccattttc
    49921 tttctctcgc aaattcgttt tggatgcact tatgtcattc acaaataccc ctgaacgcta
    49981 tggtgttata tctgcggcct tccattggct aagcgcaatt attgtctacg gcatgtttgc
    50041 tttaggtctg tggatggtca cgctcagtta ttacgatggc tggtatcaca aagcacccga
    50101 actgcataaa agcatcggta ttttgttaat gatggggctg gttattcgcg ttctgtggcg
    50161 tgtcatatct ccaccgccgg gtccgctgcc gagttattcg cccatgactc gtcttgccgc
    50221 aagagctgga catctcgcat tgtacctatt gcttttcgcc atcggcatca gcggctatct
    50281 gatctcaact gccgatggta aaccgatcag cgtttttggc tggtttgacg tccccgcgac
    50341 ccttgccgac gctggcgcac aggcagactt tgccggtgcc ctgcattttt ggctcgcgtg
    50401 gagcgtcgta gtactgtccg ttatgcacgg atttatggcc ctgaagcatc atttcatcga
    50461 taaagacgac actctgaagc gcatgctggg aaagtcgtca tctgactatg gagtataaaa
    50521 aatgaaaaaa agcctgcttg gtttaacctt cgcgtccctg atgttctctg ccggttcagc
    50581 ggttgccgcc gattacaaaa ttgacaaaga aggtcagcac gcctttgtta atttccgcat
    50641 ccagcacctt ggctatagct ggttatacgg cacctttaaa gatttcgacg gtacttttac
    50701 ctttgacgaa aaaaatccgg ctgccgataa agtgaatgtg acaattaaca ccaccagcgt
    50761 cgatactaat cacgccgaac gcgataaaca tcttcgcagt gcagatttcc tcaataccgc
    50821 aaaatatcca caggcaacat tcacctccac cagcgtgaag aaagacggtg acgaactgga
    50881 tattaccggc gatctgacgc tgaatggcgt aaccaaaccc gtcacgctgg aagcgaaatt
    50941 aattggtcag ggcgacgacc catggggtgg taaacgtgca ggcttcgagg ccgaaggcaa
    51001 aattaagctc aaggacttca atatcaagac agatttaggt ccagcttctc aggaagtgga
    51061 tctgattatt tcagtggaag gcgtacagca gaagtaagcg tgaaatgttg ccggatggca
    51121 cgcatccggc aatgatattt attctgttgg atcaggaatg cacagtgttg tattcagacg
    51181 tccacgagac ttattaaaga tcttattgcc attttcacgt cctgcccgac ggcgtcgctg
    51241 ttcttctggc ggtaacgcgc tttcttcgca gcaaatctca ctacaacaac ctttgtattt
    51301 ttccgcgcat actggacact gaataaacag caggtggcag ccatcatttt tacagttggt
    51361 atggctgtcg cacggcgcgc cgcactggtg gcaatgcgcg ataatctcat ccgatatacg
    51421 ttcgcccatc cgctcgtcaa aaacaaaatt tttgccaata aaacgcaccg gcaagccctg
    51481 ctcgcgcgcc ttacgggcgt attcaataat tccgccctcg atatgccaga ctttgttgaa
    51541 tccgttatgt ttcatccagg cactggcctt ttcacaacga atgccgccgg tgcagtacat
    51601 gacgattttt ttatctttat gtgcctgcat catctcgact gcttttggca gctgctcacg
    51661 gaaggtatct gccggaattt cgagcgcgtt ttcaaagtgc cccacttcat actcatagtg
    51721 gttacgcatg tcgataaata gtgcatcggg atcgtcaagc atggcgttca cttccgccgc
    51781 ttgcagatac tcaccaacat tgctggcatc aaagtgagga tcgtcaatac cgtcggcaac
    51841 aatgcgatcg cgtaccttca tgcgcagtac ccagaaggat ttcccgtcat catccaacgc
    51901 gatattcagg cgtaaaccct ctaaagccgg gtcgaaggca tagagctgcg cgcgaaatgt
    51961 ttcaacattg ctcgcaggta cgctgatttg cgcgttaatg ccctcatgcg ccagatacac
    52021 tcgcccaaaa acattcagcg cggtaaacag ctgatataaa gcgtcacggg tcgccttagg
    52081 atcggcgatg tggaaatact tgtaaaacga aatggtggtt cgcggttcgc tctcagccaa
    52141 cattttggct tttagcgcgt cgttggaaat gcggttgtgt aacactggca tggtgtacgg
    52201 ttcctgcgag atgggaaagt aaaaatccgc ggcatgatat agcaattatc gataattaac
    52261 atccacacat tttacgctac atttgcgcat taaaaattat ttgttattta caagcgcggc
    52321 aatttcgccc agtcttcagc cacaaatttt ggttgcgggc gaaaaaatgc gacaatacat
    52381 acaattgccc gaataggttg aaaaacagga ttgatatgac gaatctaccc aagttctcca
    52441 ccgcactgct tcatccgcgt tattggttaa cctggttggg tattggcgta ctttggttag
    52501 tcgtgcaatt gccctacccg gttatctacc gcctcggttg tggattagga aaactggcgt
    52561 tacgttttat gaaacgacgc gcaaaaattg tgcatcgcaa cctggaactg tgcttcccgg
    52621 aaatgagcga acaagaacgc cgtaaaatgg tggtgaagaa tttcgaatcc gttggcatgg
    52681 gcctgatgga aaccggcatg gcgtggttct ggccggaccg ccgaatcgcc cgctggacgg
    52741 aagtgatcgg catggaacac attcgtgacg tgcaggcgca aaaacgcggc atcctgttag
    52801 ttggcatcca ttttctgaca ctggagctgg gtgcgcggca gtttggtatg caggaaccgg
    52861 gtattggcgt ttatcgcccg aacgataatc cactgattga ctggctacaa acctggggcc
    52921 gtttgcgctc aaataaatcg atgctcgacc gcaaagattt aaaaggcatg attaaagccc
    52981 tgaaaaaagg cgaagtggtc tggtacgcac cggatcatga ttacggcccg cgctcaagcg
    53041 ttttcgtccc gttgtttgcc gttgagcagg ctgcgaccac gaccggaacc tggatgctgg
    53101 cacggatgtc cggcgcatgt ctggtgccct tcgttccacg ccgtaagcca gatggcaaag
    53161 ggtatcaatt gattatgctg ccgccagagt gttctccgcc actggatgat gccgaaacta
    53221 ccgccgcgtg gatgaacaaa gtggtcgaaa aatgcatcat gatggcacca gagcagtata
    53281 tgtggttaca ccgtcgcttt aaaacacgcc cggaaggcgt tccttcacgc tattaaatct
    53341 cccatgccgg atgcttcaga atggcatccg gcattaccac agcaaatccc cctgatttag
    53401 cgataaaagc tctctggatt gcgccccctg gaagtcgggc gcataattag tgtgcttatc
    53461 ttttcttctt atgttcaccg cgcctggcgc accaacagcg gattgctatg tcaccctgtg
    53521 aaaatgacac ccctataaac tggaaacgaa acctgatcgt cgcctggcta ggctgttttc
    53581 ttaccggtgc cgccttcagt ctggtaatgc ccttcttacc cctctacgtt gagcagcttg
    53641 gcgttaccgg tcactccgcc ctgaatatgt ggtccggtat tgtcttcagc attacatttt
    53701 tattttcggc catcgcctca ccgttttggg gtggactcgc cgaccgtaaa ggccgaaaac
    53761 tcatgctatt acgctctgcc ctcggcatgg gcatcgtgat ggtgttgatg gggctggcac
    53821 aaaatatctg gcagtttttg atcctgcggg cgcttcttgg gttacttggc ggatttgtcc
    53881 ccaacgctaa tgctcttatc gccacacaag taccgcgtaa taaaagcggc tgggcgctgg
    53941 gtacgctctc cacaggcggc gttagtggtg cgttgctcgg cccaatggct ggcggcctgc
    54001 tcgccgatag ctacggctta cgtccggtat tctttattac cgccagtgtg ctcatactct
    54061 gctttttcgt caccctgttt tgcatcagag aaaaattcca gccggtcagc aaaaaagaga
    54121 tgctgcacat gcgggaagtg gtgacatcac ttaaaaaccc gaaactggta ctcagcctgt
    54181 ttgtcactac gttaatcatc caggtggcga cgggctcaat tgcccccatt ctgacgctgt
    54241 atgtccgcga actggcgggt aacgtcagta acgtcgcctt tatcagtggc atgatcgcct
    54301 cggtgccagg cgtggcggct ctgctaagtg caccacgact cggcaaactt ggcgatcgaa
    54361 tcggacccga aaagatcctg attacagcgc tgatcttttc tgtactgctg ttgatcccaa
    54421 tgtcttacgt tcagacgcca ttgcaacttg ggattttacg ttttttgctc ggtgccgccg
    54481 atggtgcact actccccgcc gtacagacac tgttggttta caactcgagc aaccagatcg
    54541 ccgggcgtat cttcagctat aaccaatcgt ttcgtgatat tggcaacgtt accggaccat
    54601 tgatgggagc agcgatttca gcgaactacg gtttcagagc ggtatttctc gtcaccgctg
    54661 gcgtagtgtt attcaacgca gtctattcat ggaacagtct acgtcgtcgt cgaatacccc
    54721 aggtatcgaa ctgatttttc gcctttcata cttgcaaaag cggagaatca gctatccttt
    54781 tccctgaaac ctcatcaact caaagggaga atcgtgatga ccatgtacgc aacgcttgaa
    54841 gaagccattg acgctgcacg cgaagaattt cttgcagaca accccggcat cgacgccgaa
    54901 gatgcgaatg tgcaacagtt caatgcccaa aaatacgttt tgcaggacgg cgacatcatg
    54961 tggcaagttg agttttttgc cgacgaaggg gaagaaggtg aatgtttacc tatgcttagc
    55021 ggtgaagccg cgcaaagtgt ttttgatggc gactatgatg agatagagat acgccaggag
    55081 tggcaggaag agaatacatt acatgaatgg gacgaggggg aatttcagct tgagccaccg
    55141 ctggataccg aggaaggacg cgcagcagct gatgagtggg atgaacgtta atcactcata
    55201 cgggccatga tgaatgtcga tcggcagcag taaagtatca aaaaccagag agaatggcag
    55261 atcaaggatc gtgacataac gccaggcgga gtcacgcaca tcccattgca caccgggata
    55321 atattggttg ccatgcccct gccccggtat agtgcgacta ataatgctgc cacagccact
    55381 cagcaaggtc accatgatgc tcaccacaat taaacgcatt tgatacctct ttcgtaaaaa
    55441 aataccggca taacgccggt atttttattg gctcgttgtt tttgccggat gcggcgtgaa
    55501 cgccttatcc gacccacaaa gtcttgcaaa atcaataaat tgcaggaacg atgtaggcct
    55561 gataagcgta gcgcatcagg caactacgtt tttattgcga agccgcatcc ggtttacgca
    55621 atgccagtgg attgagcttt atcccttcgt aataactcac ccatgaagaa tatctctccg
    55681 gggaattcca gacacggaaa tgcagacggg ccatcgtcac cggatcgctt agcagcacca
    55741 ggcgacgatc gcgattcagc ttctctggcg tctcgttcag cgcctgttca acgtggcggt
    55801 cacgggcgat ttccagcacc ttactggcgc ggtgacgcgc ggtcgccatt gcggttgcca
    55861 gagcgttaaa tgacgggtta aacactgcgt gcataaagcc atcatcaagg gagcgttgac
    55921 gattcatctc aaggaaccga tcggtatcaa ccagcacctg cggcggcgaa tactcttccg
    55981 ggatcaggaa cagtttccag cgtttggtgc gcagaccaac ggtggcacgg ctggaaatca
    56041 ccgaaacaaa cggtgacagg atcaacgaga agacaatcgg tgccagccag aacaggaaac
    56101 gcagatccag ccacgccatc ccaacagccc acactaaccc taacagcagc tgtgagccgt
    56161 ggcgtttgaa cgcttcaccc caggaagtgg agtcatcatc acgctgcggt gaattccaca
    56221 ccacttccca gccaaggaac gcgctgacaa cgaagaccgt atggaacagc atgcgtaccg
    56281 gagccagcag cacggaaaaa agcacttcca gcagcaacga taatgtaacg cgccagaagc
    56341 cgccgtattc tttcgttcct ttgcaccaga taagcaaaat gctcaataac ttcggcagga
    56401 acaacagcac catggtcgaa gcaaaaagtg caatcgccag ctcaggacgc cactgcggcc
    56461 acactgggaa caactgccgt ggttgcagga agtattgcgg ttcggtcaac gcatgcacta
    56521 cctgcaatgc agtagagagc gcgaggaaca taaaccacag cggagcggag agataagaca
    56581 tcacgcccgt caggaacacc gcacggtgaa ccgggtgcat acccttcacc aggaacagac
    56641 ggaagttcat caggttaccg tggcaccatc ggcggtcacg ttttagctca tcaagcaagt
    56701 taggcggcaa ttcttcataa gaacccggga gatcgtaagc aatccagacc ccccaacctg
    56761 cacggcgcat caacgccgct tccacgaagt catgtgacag gattgaaccg gcaaaggaac
    56821 cttcgcccgg cagcggagcc agtgcgcagt gctcgataaa cggtttcacg cggataatcg
    56881 cgttatgtcc ccagtagtgc gactcgccaa gttgccagaa gtgcaaaccg gctgtaaaca
    56941 gtggcccata cacgcgggtc gcgaactgct gacagcgcgc atacagcgta tccataccgg
    57001 acgctttcgg cgacgactga atgatcccgg cgttcgggtt ggcttccatc aggcgcacca
    57061 gcccgcacaa acaatcaccg gtcattaccg agtcagcatc cagcaccacc atgtagctgt
    57121 actggctgcc ccagcgacgg cagaagtcat cgatattacc gcttttacgc ttcacgcggc
    57181 gacggcggcg gcgatagaaa atctgacctt cgccaccgac ttcagcgata agctccatcc
    57241 aggctttttg ctctgcgacg cagatatccg ggttataact gtcactaaga atgtagacat
    57301 caaagtgttt ggcattcccg gtggctttta ctgattccca cgttgcacgc aggccagcaa
    57361 aaacacggtt cacgtcttcg ttacagatag gcatgatcaa cgccgtgcga tgctccgggt
    57421 ttaatggttc atcgccaact gttgacgcag atatactgta tttatcgcga ccaataagca
    57481 gttgcaggaa gcccattaac gccgtccaga atccggcgga cacccaacag aacagtaccg
    57541 caaagaggat caggataccg gtttgcagca tataaggcag aagctgcata aaggaaaccc
    57601 acaaatcctg accaaccata tccataggat taatcagcgc ccaaccctga taaggaagaa
    57661 tggtcttcat ataccaggtc gcgacgacag tttgcgcgag cgtcaggatc aacagaatgt
    57721 aacggcggat ggtaccgacg gtacgccact tttgctcgct ctcctgctct tctttggtca
    57781 aacgagccag atagcgcggc gtgacatcgc gtccacgcag gcgatcccag aaacggccta
    57841 ccgggttggt acgccacggg tcgggaaaca tcgaggagcg ttttgcttct ggcatcgcct
    57901 tcagctgatc gcgcccttcg tcgtctttaa ttaactgtcc atcagcaagt gaatctggcc
    57961 aggcttgttc cagacgcgcc tttaccgagc cttgcgggga atcatcctcc cgcgcccagg
    58021 tgcggtgttc ggcatccagc gcctgatgaa cggcgcggat atcagtcttc ggcaatgccg
    58081 ctttctcgct tgcggcgatg ggcattgcgt caatgtactc agttgtctta ttcattggca
    58141 ggtaactggt agctccaggt ttcactcaac gtctgatcgg cattcaccag cgcagcacgc
    58201 atttcagtgg ttttcttggc atctttcact ttcacacgca tcaccagacg ccagcctttg
    58261 gtaaccgggt tatagcgcac cgtgctttca actatctcac cattatcacc aatgctggtt
    58321 tgcgctgtga ccggggtatc ctctggcagt tttttcatct ctgcgccggt aaaatcgacc
    58381 acaaaggcga tagtaccgtc aggctggcga atcaggttcg actgcttcac atcccccgtt
    58441 gaacgacgcg tttgttgcac ccatgcgtta tctggcgcat gcagtttgtc ttcatcacgg
    58501 ctgaaggtga tggtgtattt aaagttcatc tctttacccg gctccggcag ctgatccggc
    58561 gtccagtaag cgacgatgtt atcgttggtt tcatcgttgg ttggaatttc caccagctca
    58621 acgctgcctt taccccactc ccctttcgga gtcacccatg cgcttggacg gagatcgtaa
    58681 cgatcatcga gatcttcaaa gcgggagaaa tcacgaccgc gctgcaacag accaaagcct
    58741 tgcgggtttt ccatggagaa gctgctgacc gctaaatgtt tcgggttatt caacggacgc
    58801 cagatccatt cgccattacc ggcatggata gagagaccgt tagagtcgtg caactccgga
    58861 cgatagttat ttgcaggcga cggttggttc ggcccaaaca ggaacatact ggttaacggt
    58921 gcaaccccca gtttgccgac tttatcgcgc agatagattt tcgactgcac atccacaacc
    58981 gtgtcacgcc ctggcataac cacgaatttg taagcacctg tcgcacgcgg cgagtcaagc
    59041 aatgcataga tggttaaacg tttatcagtc ggttttggac gctcgatcca gaactctttg
    59101 aagcgcggaa attcttcacc cgatggcaag gcggtatcaa ttgccaggcc gcgggcagaa
    59161 aggccataaa cctgacctgc accaatcacg cggaaatagc tggccccgag catgctgacg
    59221 atttcatcgt ttttatcttt gctgttgatc gggtaaagca ctttaaaacc ggcaaagcca
    59281 aggtctttta ccgtatcttt gtcatgctga acatcgccga aagtgaaata atccgggctg
    59341 tatttgattc gtttgactgc ggtggcagtc acttcattta ttttgaccgg ggtatcgaag
    59401 tacataccct gatggtagaa ctcgagtttg aatggggtct tcagattgtt ccagtacgct
    59461 ttgtcatgat taaactggat ctgctgatag tccgcgtatt tcatatcgcg gaaaacggag
    59521 ggcaagttgc ttttgggcgt ctcgtagcct ttcccggcta aagattgagc ttgctttgcg
    59581 acatcatcaa tactgaaagc ccagcttgaa gatgtataca gggttaacat tactgcagca
    59641 ctcaaccaac gcattttcat catttgtagt ttatgtttca taattagtaa gcacttcccc
    59701 ctttgtgtgc ttatatcgat ccgatctatt ttaatggata accagccatt ccgacaacag
    59761 aatcccggta tcgttcagca cgctggctca tggattaagc acctgattgc aaccgttttc
    59821 actttccgtg cttctcctaa agacagaagc atcgacttcg tgtagggttt gccagcaaat
    59881 ttatttatca accgtcttac agacgcttcc attctggtta gtggtagcga tgatgctatg
    59941 cataaaaacc ttgaagcctg cgcctggcgc ggtcaggtcg gtgtcactat ggcaacaagc
    60001 ctaaaggcat tacccgacag ggataagacg aaaaatccga gattagttaa ccatatatga
    60061 acccagtacc cgcgcaacgt gaatatttcc tcgactccat ccgcgcctgg ctgatgttgt
    60121 tagggatacc ttttcatatt tctttaatct attcgagcca tacatggcat gtgaatagcg
    60181 ccgaaccatc attgtggctg acccttttta atgacttcat ccactcgttc cgcatgcagg
    60241 tatttttcgt tatatccggc tacttttcct acatgctttt tttacgctat cccttgaaaa
    60301 aatggtggaa agtacgtgtc gaacgtgtag gtatcccgat gttaacagcc atccccctac
    60361 tgacattacc gcaatttatt atgctgcaat atgtcaaagg aaaagcggaa agttggcctg
    60421 ggctgtcatt gtatgacaaa tataatacgt tggcctggga attaatatca cacctgtggt
    60481 ttttactggt gttagtggtc atgacgacgc tgtgcgtatg gatatttaag cgcatcagaa
    60541 ataatttaga aaattctgat aaaacgaata aaaattctcg atggtaaaac tatcggtgat
    60601 ttttttatgc ctcggcatcg gttatgcggt aataagaaga acgattttta ttgtgtatcc
    60661 gcccattctg agtaatggca tgttcaattt tattgtcatg caaacgctgt tttatttgcc
    60721 gttctttatc ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac
    60781 gccgtctcgt ggctgtaccc ttgcagcagc attggcgttt gtcgcttatt tactcaacca
    60841 gcgctatggc agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct
    60901 cggtctgtgg atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc
    60961 agcgcgggtg acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt
    61021 aacgctgttt ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct
    61081 ctgtggcctg atatttgtag tagggattgc gataattctg tatgaaattc atttgcgcat
    61141 cccgttactg aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc
    61201 accagcccgt taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg
    61261 accataaccc gcttccagaa actggtggcg ggttcttttt tgagaataat ctctttcttt
    61321 gcatgacgat caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca
    61381 tcatactggc tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc
    61441 acgaagccca tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata
    61501 aacaccgttt taccatcgat gctaaaggtt ttagcatgca ggctggctcc ggaattaccg
    61561 gttatgccgc gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ttcatataat
    61621 tccacgccat agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca
    61681 acatcgttag cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc
    61741 cgtaagagtt gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg
    61801 cgttcactgg gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt
    61861 gccttgccct cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc
    61921 ggcaatgttc catcaaccag atgatttata aatggactgg attccatttt gcgtaaataa
    61981 cgatgcgtca tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca
    62041 ccctccggga catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg
    62101 gcgaaatcat cggcaacgtc ctctaccacg ggtcctattg ccatgacatc taaatccgaa
    62161 aaaagtggct cctcccctgc tccaaaatag gcatcaccaa tatttcgtcc tcccaccagg
    62221 gtcaccacgc catcgacagt gaaactttta ttgtgcatac ggcgattaag acgggaaaag
    62281 tcggtgatat aaccaagcgg acgcagcaag cgaaacgaga aaggattaaa aagccggact
    62341 tcaatgcgtg gatgactgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg
    62401 tcgtccagca acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac
    62461 agtaaccgac ccgacatatc gtcctgccag atgtaatact gaacatcgag cgtatgctct
    62521 gccatttcgg ccagacgata ccgggcggca aacgcatcca gacttttctc cagaggaaaa
    62581 aggccacact gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc
    62641 aaatcattca tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc
    62701 ataacagaca aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt
    62761 ttttactgcg atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc
    62821 aggaaaagcc actgacgtat agctgtttgc cgccaccagt cgtaggctat tgagataggc
    62881 atcctgcaaa agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg
    62941 cactacggct ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca
    63001 atcgccctgc tgttgcctga cttttaaaca ggcatccagt agggccggac ccgctgcgcg
    63061 atgaatggcc ccatcgacgc cgccacctcc cattaatgaa ggattagccg cattcacaat
    63121 cacatcaacg gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac
    63181 gcctccttac accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt
    63241 ttctactcag caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca
    63301 gctttttctg tttatcaata tactgcactt ttactgaggc tacttgcgcg tttttgacat
    63361 tgccgctcat taacgctttg ctggaatagg tttttcccgg aacagaacca ttctgtgggc
    63421 agttcatttc tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc
    63481 cataaaaatg gatcacgccc ccttgcgcgg tgcttcctgc gatggcaata aaaggaaaaa
    63541 acaaactgcc gagcataaga gagtttaaaa aaggtctgaa catttattct atttcctcaa
    63601 tgattagtca tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag
    63661 accattctct ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag
    63721 agggcggcca ttgttgtgat aaatgaagtg actgtccatc agaaacagta acaactattt
    63781 tcacccgatc gtccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag
    63841 caggcaatga aagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca
    63901 gggacaatat ttgtactctg cacagacaag attgagtaag agtgacttca ggaataatgg
    63961 tatacacatc cccttgctgg gtcgtattaa aggttatctg actggaaagt gccgcaagga
    64021 gtaataacgt attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta
    64081 atacaaatga tgtattagta ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc
    64141 acgttgacgg aggagttaga tgcagtctgg tcaactgcag caccgttgcc accaccgaac
    64201 tgtttaaccg tcatttcaga atttttgccg ttccactgat caagagtagc gctgttaccg
    64261 aagccacgtt gggtcagatc gattgagctg tcatctgagc cctgaccaac atctgcacca
    64321 ttaccgccgc catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca
    64381 agtgcagagt taccgccacc gtactggtaa atgttcagct cagaatttgg gccgctatta
    64441 ttaccgccac caccgtggtt accgccgccg ccgtactgag gaacaacacc tgccagagcg
    64501 ctaccggaga atacgattgc tgcaattgct gctactttta aaagtttcat gtaaaacccc
    64561 catcggattg atttaaaagt cgaatggaaa ttaacgttgt gtcacgcgaa tagccatttg
    64621 cgactgtctc tgcactacaa ttgccgtttt ttgagtacca tactgtgtaa tatttgcttt
    64681 attaccagaa cctttctgga taatcatcgc agtattacca taagcacctt gcgaaatact
    64741 ggcatcgttg gcactgcccg cctgatcaat atatgcaagg ttataatctc ctgtctggtc
    64801 aatctttgcc cggttgctac taccttcttg cgcaacaacc gccaaaagtt ttgagcctcc
    64861 ctgccgtaac tgagcactat tattagtccc agcttgacca attatggctg cctgattaaa
    64921 tgaagactta ctcaattcat ttaccgcgaa gttatattct gaattagcta aatcataacc
    64981 tgctgcggct gcaatcccag gcgcacccag tattgttaac atcataaata acaatttgtt
    65041 tttcatgttg tcaccctgga cctggtcgta catttaagaa attaaatcat ttcaacttgg
    65101 ttgttaacgc aacctgtatt ttgttaacgc tgcgttacga tggaaagtat gtctgcggaa
    65161 atatttttaa taactcaccc gcgcgttgta tttttctttt ttagttcata ccaaagtatt
    65221 aaaacccacg taaatacg
//
LOCUS       DAJOSY010000012        63697 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJOSY010000012 DAJOSY010000000
VERSION     DAJOSY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.012"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            67..567
                     /locus_tag="OHN27_000846"
     CDS             67..567
                     /locus_tag="OHN27_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2285342.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            658..3342
                     /locus_tag="OHN27_000847"
     CDS             658..3342
                     /locus_tag="OHN27_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCQ2285343.1"
                     /translation="MDKKLLALLILASLSPAEAALTKIPAGFEVIAQGQQEYIEVYFS
                     GKNLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQASDDESSAYQLGVRLPLHPQYELYAGLANA
                     DNVSAFELGNDWTANLGGAGNLAISASVFRNDDGGKGDMQQANWSNSGWPTLGFYRTN
                     SDGDACATDSRESYNALSCYESISATVSQNFVGWNMMLGYSRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSDQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPASVEK
                     FPLVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            3339..4427
                     /locus_tag="OHN27_000848"
     CDS             3339..4427
                     /locus_tag="OHN27_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCQ2285344.1"
                     /translation="MRNRFIAAILGLFGAVTGVQAAPNVTSEITYDLANGRADYYFWN
                     EEPPPEVSYSTTFSFFQCSYPDSQQTCTSAGNTSVVQIYLTEKRSGMRWPVKLKGYMT
                     VQVWEDGPCKGWYDKKRLDDGTGYQCKDTINNVGYLAKTKVLTLYIEQEEMKKLPIGG
                     LWEGKVKLHFSYPATDYQADIKLNVLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVE
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4515..5813)
                     /gene="exuT"
                     /locus_tag="OHN27_000849"
     CDS             complement(4515..5813)
                     /gene="exuT"
                     /locus_tag="OHN27_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCQ2285345.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6296..7708
                     /gene="uxaC"
                     /locus_tag="OHN27_000850"
     CDS             6296..7708
                     /gene="uxaC"
                     /locus_tag="OHN27_000850"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCQ2285346.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7723..9210
                     /gene="uxaA"
                     /locus_tag="OHN27_000851"
     CDS             7723..9210
                     /gene="uxaA"
                     /locus_tag="OHN27_000851"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCQ2285347.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9293..9844
                     /locus_tag="OHN27_000852"
     CDS             9293..9844
                     /locus_tag="OHN27_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCQ2285348.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAVRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9849..11093)
                     /gene="sstT"
                     /locus_tag="OHN27_000853"
     CDS             complement(9849..11093)
                     /gene="sstT"
                     /locus_tag="OHN27_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCQ2285349.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(11492..12457)
                     /gene="alx"
                     /locus_tag="OHN27_000854"
     CDS             complement(11492..12457)
                     /gene="alx"
                     /locus_tag="OHN27_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCQ2285350.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(12740..13726)
                     /locus_tag="OHN27_000855"
     CDS             complement(12740..13726)
                     /locus_tag="OHN27_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCQ2285351.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKINVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAQGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(13805..14497)
                     /locus_tag="OHN27_000856"
     CDS             complement(13805..14497)
                     /locus_tag="OHN27_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCQ2285352.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATILIADRVMVNAS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAQK"
     gene            complement(14575..15078)
                     /locus_tag="OHN27_000857"
     CDS             complement(14575..15078)
                     /locus_tag="OHN27_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCQ2285353.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKVEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15163..16299
                     /gene="rlmG"
                     /locus_tag="OHN27_000858"
     CDS             15163..16299
                     /gene="rlmG"
                     /locus_tag="OHN27_000858"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCQ2285354.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPQLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            16584..16898
                     /gene="higB"
                     /locus_tag="OHN27_000859"
     CDS             16584..16898
                     /gene="higB"
                     /locus_tag="OHN27_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCQ2285355.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16895..17311
                     /gene="higA"
                     /locus_tag="OHN27_000860"
     CDS             16895..17311
                     /gene="higA"
                     /locus_tag="OHN27_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCQ2285356.1"
                     /translation="MITIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17356..19374)
                     /gene="fadH"
                     /locus_tag="OHN27_000861"
     CDS             complement(17356..19374)
                     /gene="fadH"
                     /locus_tag="OHN27_000861"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCQ2285357.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDNFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVAEDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHLKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVMINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19800..22151)
                     /gene="ygjK"
                     /locus_tag="OHN27_000862"
     CDS             complement(19800..22151)
                     /gene="ygjK"
                     /locus_tag="OHN27_000862"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCQ2285358.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GHWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNEHNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTTDGPIQENYNPLTGAQQGAPNFSWSAAHLFMLYNDFFRKQ"
     gene            complement(22168..23238)
                     /gene="ygjJ"
                     /locus_tag="OHN27_000863"
     CDS             complement(22168..23238)
                     /gene="ygjJ"
                     /locus_tag="OHN27_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HCQ2285359.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            complement(23372..24805)
                     /locus_tag="OHN27_000864"
     CDS             complement(23372..24805)
                     /locus_tag="OHN27_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2285360.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(24868..25317)
                     /locus_tag="OHN27_000865"
     CDS             complement(24868..25317)
                     /locus_tag="OHN27_000865"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCQ2285361.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25314..28406)
                     /gene="ebgA"
                     /locus_tag="OHN27_000866"
     CDS             complement(25314..28406)
                     /gene="ebgA"
                     /locus_tag="OHN27_000866"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCQ2285362.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28590..29573)
                     /gene="ebgR"
                     /locus_tag="OHN27_000867"
     CDS             complement(28590..29573)
                     /gene="ebgR"
                     /locus_tag="OHN27_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCQ2285363.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29792..30124
                     /locus_tag="OHN27_000868"
     CDS             29792..30124
                     /locus_tag="OHN27_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCQ2285364.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGQKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30166..31545)
                     /gene="ygjG"
                     /locus_tag="OHN27_000869"
     CDS             complement(30166..31545)
                     /gene="ygjG"
                     /locus_tag="OHN27_000869"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCQ2285365.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            31963..33483
                     /gene="aer"
                     /locus_tag="OHN27_000870"
     CDS             31963..33483
                     /gene="aer"
                     /locus_tag="OHN27_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCQ2285366.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVAHQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33637..34260)
                     /gene="nfeR"
                     /locus_tag="OHN27_000871"
                     /gene_synonym="yqjI"
     CDS             complement(33637..34260)
                     /gene="nfeR"
                     /locus_tag="OHN27_000871"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCQ2285367.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            34548..35312
                     /gene="nfeF"
                     /locus_tag="OHN27_000872"
     CDS             34548..35312
                     /gene="nfeF"
                     /locus_tag="OHN27_000872"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCQ2285368.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35366..35441)
                     /locus_tag="OHN27_000873"
     tRNA            complement(35366..35441)
                     /locus_tag="OHN27_000873"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35406..35408),aa:Ile,seq:cat)
     gene            35566..36072
                     /gene="mug"
                     /locus_tag="OHN27_000874"
     CDS             35566..36072
                     /gene="mug"
                     /locus_tag="OHN27_000874"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCQ2285369.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(36151..37992)
                     /gene="rpoD"
                     /locus_tag="OHN27_000875"
     CDS             complement(36151..37992)
                     /gene="rpoD"
                     /locus_tag="OHN27_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCQ2285370.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(38187..39932)
                     /gene="dnaG"
                     /locus_tag="OHN27_000876"
     CDS             complement(38187..39932)
                     /gene="dnaG"
                     /locus_tag="OHN27_000876"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCQ2285371.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(40043..40258)
                     /gene="rpsU"
                     /locus_tag="OHN27_000877"
     CDS             complement(40043..40258)
                     /gene="rpsU"
                     /locus_tag="OHN27_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCQ2285372.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40496..41509
                     /gene="tsaD"
                     /locus_tag="OHN27_000878"
     CDS             40496..41509
                     /gene="tsaD"
                     /locus_tag="OHN27_000878"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCQ2285373.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(41552..43015)
                     /gene="ttdT"
                     /locus_tag="OHN27_000879"
     CDS             complement(41552..43015)
                     /gene="ttdT"
                     /locus_tag="OHN27_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCQ2285374.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(43063..43668)
                     /gene="ttdB"
                     /locus_tag="OHN27_000880"
     CDS             complement(43063..43668)
                     /gene="ttdB"
                     /locus_tag="OHN27_000880"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCQ2285375.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(43665..44576)
                     /gene="ttdA"
                     /locus_tag="OHN27_000881"
     CDS             complement(43665..44576)
                     /gene="ttdA"
                     /locus_tag="OHN27_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCQ2285376.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            44783..45715
                     /gene="ttdR"
                     /locus_tag="OHN27_000882"
                     /gene_synonym="dan"
     CDS             44783..45715
                     /gene="ttdR"
                     /locus_tag="OHN27_000882"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCQ2285377.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(45728..46345)
                     /gene="plsY"
                     /locus_tag="OHN27_000883"
     CDS             complement(45728..46345)
                     /gene="plsY"
                     /locus_tag="OHN27_000883"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCQ2285378.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46450..46818
                     /gene="folB"
                     /locus_tag="OHN27_000884"
     CDS             46450..46818
                     /gene="folB"
                     /locus_tag="OHN27_000884"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCQ2285379.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            46908..47729
                     /gene="bacA"
                     /locus_tag="OHN27_000885"
     CDS             46908..47729
                     /gene="bacA"
                     /locus_tag="OHN27_000885"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCQ2285380.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(47910..49148)
                     /gene="cca"
                     /locus_tag="OHN27_000886"
     CDS             complement(47910..49148)
                     /gene="cca"
                     /locus_tag="OHN27_000886"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCQ2285381.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQKMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(49212..49832)
                     /locus_tag="OHN27_000887"
     CDS             complement(49212..49832)
                     /locus_tag="OHN27_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCQ2285382.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            50074..51375
                     /gene="ygiF"
                     /locus_tag="OHN27_000888"
     CDS             50074..51375
                     /gene="ygiF"
                     /locus_tag="OHN27_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCQ2285383.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            51398..54238
                     /gene="glnE"
                     /locus_tag="OHN27_000889"
     CDS             51398..54238
                     /gene="glnE"
                     /locus_tag="OHN27_000889"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCQ2285384.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALCNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            54286..55719
                     /gene="hldE"
                     /locus_tag="OHN27_000890"
     CDS             54286..55719
                     /gene="hldE"
                     /locus_tag="OHN27_000890"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCQ2285385.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(55839..55898)
                     /gene="ibsE"
                     /locus_tag="OHN27_000891"
     CDS             complement(55839..55898)
                     /gene="ibsE"
                     /locus_tag="OHN27_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCQ2285386.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(56214..56273)
                     /locus_tag="OHN27_000892"
     CDS             complement(56214..56273)
                     /locus_tag="OHN27_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCQ2285387.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(56512..58173)
                     /locus_tag="OHN27_000893"
     CDS             complement(56512..58173)
                     /locus_tag="OHN27_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCQ2285388.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFTRLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESISLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPTVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGDASTGSVSGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLTALTSPLTSTTPVAENVE"
     gene            complement(58200..58829)
                     /locus_tag="OHN27_000894"
     CDS             complement(58200..58829)
                     /locus_tag="OHN27_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCQ2285389.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALLYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            59098..59298
                     /gene="glgS"
                     /locus_tag="OHN27_000895"
     CDS             59098..59298
                     /gene="glgS"
                     /locus_tag="OHN27_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCQ2285390.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(59341..60405)
                     /locus_tag="OHN27_000896"
     CDS             complement(59341..60405)
                     /locus_tag="OHN27_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2285391.1"
                     /translation="MRYLLTVIAFIMGFSALPVWAMNCYAEHEGGNTVVIGYVPRIAI
                     PSDGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWVNIMMLLASGQDFYNQNSY
                     TLGVTYNGVDYDSTSTRAIAAPECIDVKGAGIYSNTYKNPAVCSGGPEPQLSVTFPVR
                     VQLYIKLAKNANKVNKKLVLPDEYIALEFKGMSGTGTIDTDKNLTFRIRGLNNIHVLD
                     CFVNVDLEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDIGNGLLLKIFHNDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(60407..61156)
                     /locus_tag="OHN27_000897"
     CDS             complement(60407..61156)
                     /locus_tag="OHN27_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCQ2285392.1"
                     /translation="MRYLNTKNIIAAGVLLSCMNSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKKLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPNEQIQIRMITQEKIAQL
                     PKDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMTYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGVTMLPQTQSLMVLPFSHADTQFKNVPSNFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            complement(61178..63676)
                     /locus_tag="OHN27_000898"
     CDS             complement(61178..63676)
                     /locus_tag="OHN27_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2285393.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVIIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLSTLINNKRTPIHLDLQWVLIDNLTAVCVTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFSITNLFNTLQGQLDVKVEEE
                     DGRVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLATGVGFNLNSLGSLSFDVTRSDAQLHNQNKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVSMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEDDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNNISHTASWYDSSDRNNSWSVSASGDNDEFKDMEASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDETSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPKSSNLTTGTVILPCIS"
BASE COUNT        15435 a        15944 c        16739 g        15579 t
ORIGIN      
        1 caaagataat ggcgagtttg aactggcggt ccattaatta tcattgtaat aaacgaagga
       61 atagtaatga aaaaggtatt tgcaaaatct cttctggtcg cagcgatgtt ttctgttgca
      121 ggctccgcat tagcagtaca aaaggatatc actgtaacgg ctaacgttga tgccgctctg
      181 gatatgacac agaccgataa cactgcgctg ccgaaagcag tagaaatgca atatctgccg
      241 ggtcagggtc tgcaatctta ccagttaatg accaaaatct ggtctaacga cgttaccaaa
      301 gatgtaaaaa tgcagttagt ttccccggcg caactggttc aaagcctgga cgctagcaag
      361 attgttccgc tgacagtaac ctggggcggc gaagaaatta aagctgatgc ggcaaccact
      421 tttaccgcaa ccaaaatctt tgcttccgat gcgttaacta atggttctct ggctaaaaat
      481 ctgatgtttg ctcaaaccac caaaggtgtt ctggaaacag gtatctaccg tggcgtagta
      541 agcatttatc tgtctcagga tatctaatcc ggataacaga attaacacat aacaaagggg
      601 ggctagccct cctttatttt aactgtttta gtatttgtta tggatttcag gattgccatg
      661 gataaaaaat tactggctct tttgatcctg gcgagtctca gcccggcaga ggcggcatta
      721 accaaaatcc ccgcagggtt tgaggttatt gctcagggac agcaggagta tatcgaggtt
      781 tatttttcag ggaaaaatct cggtaaatat tatgcaatgg ttaatcttga taccgtaaca
      841 tttcttgatc cagcaagttt atataacaag ctggaactgg atgtcgacga tcagaaaatc
      901 gcgcatatag tgaaagaaaa attatcgcag ccgctagctc gccacggtga attggcttgc
      961 ggttatgtac gtactgactc agggtgtggt tttctgaata ccgatacgct ggaaataatc
     1021 tataatgatg aagaaagttc ggcaacgttg tttattaatc cgcaatggaa ttcagctttc
     1081 gatgcgaagt cattatattt aaatccagac aaaaatacgg ttaacgcttt tatacatcag
     1141 caagacatca atgttctggc acaggatgat taccaatcgt tgtctattca gggaaacggt
     1201 gcgctgggaa taacagaaaa tagctatatt ggtgcacact ggaatttcaa cggttatgat
     1261 gcagatgatg tcagtgacag taatgctgat gtcagcgatc tctattatcg ttatgatttt
     1321 ttacgtcgtt attatgtgca ggcggggcgc atggacaacc gcacgctatt taatgcacaa
     1381 ggcgggaact ttacctttaa ctttttgcca ctcggtgcaa tcgacgggat gcgtatcggg
     1441 tcgactctta gctatttaaa ccaggcgcaa agccagcagg gaaccccggt aatggtcctg
     1501 ctttcgcgca attctcgtgt tgacgcttat cgtaatgagc aacttctggg atcgttttat
     1561 ctcaatagtg gttcgcaatt tattgatacc agttcctttc cgccgggtag ctatagcgta
     1621 gcgttaaaag tctatgaaaa taaccaactc acccgcaccg agctagtacc gtttaccaaa
     1681 acaggcggtc tgacggacgg aaatgcgcaa tggttcttac aggcaggtaa aactacatca
     1741 caggcttctg atgatgaaag ttcagcttat caactggggg tacgcctgcc attacatccg
     1801 caatatgagc tctacgcagg gctggcgaat gccgataatg tgagtgcttt cgagttaggt
     1861 aatgactgga cggcaaattt aggcggggca gggaatcttg caatcagcgc cagcgtgttc
     1921 cgtaacgatg acggcggcaa aggtgatatg caacaggcca actggagtaa ttcgggatgg
     1981 ccgacgttgg gcttttatcg gaccaactct gacggcgatg cttgtgcaac cgacagcaga
     2041 gagagctata acgccttaag ctgttatgaa agtatttccg cgacggtttc acagaatttt
     2101 gtcggctgga atatgatgct gggttattcc cgcacacaaa ataacactga tgatagtttg
     2161 cgttgggata aacagcagag ctttgaaaat aactatcttc gccagacaac tgcgcaaagt
     2221 atctccgaaa ctgtacaact tagcgcttcc cgcgcttttg tgatgcgtga ctggatcttg
     2281 agtacttcgg ttggtgtttt ccatcgtaat gacaacggtg gcgataacga cgacaacggc
     2341 ttgtacttat cgttttcgtt atctgacacg ccaacgatgg acagcaataa caacagccat
     2401 tcaaccaatg tttctacgga ttatcgttat agcgatcagg atggcgatca aacgtcatgg
     2461 cagttatccc atacttttta taacgattca ttcagccata aagaacttgg cgtaaccgtt
     2521 gggggcctga acaccgatac cataaacagc gcggttaacg ggcgttggga tggtcaatac
     2581 ggaaatgtct acgctaccgt atctgacagt tatgaccgta agaatcatga tcatctctcg
     2641 gcctttacgg ggacttacag ctctacactg gctgtcagtc gctatggcgt taatttgggt
     2701 gccagtggta cagacgattt gctgggtgcg gtattggtgg atgtgaaagg cttctctgaa
     2761 caggatgaag agagtcagga tctgcaactc gaagcgcggg tggcaggcag ccgaacgttg
     2821 cagcttggtc aaagcgacag tgtgttgttc ccttatcctg gatttcagtc tggttttgtt
     2881 gaggttaacg acagtagcca gggcaatcaa caagggacaa caaacatcat taacggtgcg
     2941 gggaatcgtg aattaatgtt gttgcctggc aaactgcgct atcgcgaagt gtctgccagc
     3001 tttaattaca actatatcgg tcgcttgtta ttaccagcat cggtagagaa attcccgctg
     3061 gttggtctga atagcgccat gttactggta gctgaagatg gcggatttac acttgaaatt
     3121 aacggtagcg aaaaagagct atatctgctt tccgggcagc aattccttaa gtgtccgttg
     3181 agtgttgtaa agaaacgcgc cagcattcgt tacagcggag atgtcacttg tagtgtggtg
     3241 acttattcac aattaccgga atccattcag gttcaggcac agttaaaaca gcctaaatta
     3301 cgtggaaacg ttcagacggc gcaaagggag gttgcaccat gagaaatcga ttcattgcgg
     3361 cgatattggg cttgtttggc gcggttactg gcgttcaggc ggctcctaac gtgaccagtg
     3421 aaattacgta cgatttggca aatggcaggg cggattatta cttctggaat gaggagcccc
     3481 caccggaggt gagttacagt acaacatttt cattttttca atgtagctac cctgattcac
     3541 agcagacttg tacatcagca ggtaatactt ctgtcgtgca aatttatcta actgaaaaac
     3601 gcagcggtat gcgctggccg gttaaactga aagggtatat gacagttcag gtgtgggagg
     3661 acggaccgtg taaggggtgg tacgataaga aaaggctgga tgatgggacg ggttatcaat
     3721 gtaaagatac gattaataac gttggttatc tggctaaaac aaaagtttta actctgtata
     3781 ttgagcaaga agaaatgaag aaactgccga ttggcggttt atgggaaggg aaagttaaac
     3841 tccattttag ctacccggca acagattatc aggctgatat taagcttaat gttctcgacc
     3901 ccaaccatat cgacgtgttc ttcccggagt tcgcccacgc cacgccacgg gtgcagttag
     3961 acttgcatcc aacagggagc gttaatggca gcaactacgc gcaagatctg accatgttgg
     4021 acatgtgctt gtacgatggt tttaacggta atgccatcag ttatgaaatc atgctcaaag
     4081 atgaagggcg acccgccgca gggcgcagag acggtgactt ctctatctat cgtcagggag
     4141 gaaccaccac cgacgaggga gaacgcattg attaccgggt caaaatgtac aacccggaaa
     4201 ccggtgggca aattgatgtg cgcaataatg aaaatatggt ctggaacagc attaacctga
     4261 aacgtgtgcg tccggtcgta ttgccaggga tccgctatgc cgtaatgtgt gtgccaacgc
     4321 cattaacgct ggcagtagaa aaatttagtg tgatggacaa acaggctgga tattacatgg
     4381 ggaaattgtc ggtaatcttt acgccttcct tgccaaccat caattaaccc tgcaaaccgg
     4441 acaaacgctt ttgtctatat aaacaatgaa gccgcctgat aaggcggctt cgtcattaca
     4501 acttacgtta aatgttaatg ttgcggtgcg ggatcgttat gtgtttcctg cgccacctca
     4561 atcgccggtt tgttctgcaa cacggtccag ataaccagcg cacctaacag gtcgaacact
     4621 gccagtactg cgaacagcgg gctgaagccg atggtgtcag ccagtgcacc gacaaccagc
     4681 gcaaatagcg tacttgccag ccatgcggac atcccggtta aaccgtttgc cgttgccact
     4741 tcgttacgac caaacacgtc agaagagagc gtaatcagcg cgccagacag tgcctggtga
     4801 gcaaaaccac cgatacacag cagcataatt gcgacatacg ggttggtgaa cagaccgatc
     4861 atacccgggc caatcatcag cactgcaccc agcgttacga ccatcttacg ggaaacgatc
     4921 aggttcacac caaaccaacg ctggaacagc ggcggcaggt aaccaccgag gatacaaccg
     4981 aggtcagcaa acagcatcgg catccaggcg aacatcgcga tctctttcag gttaaagccg
     5041 taaactttaa acatgaacag cgggatccac gcgttaaaag taccccaggc aggttctgcc
     5101 aggaaacgcg gcagcgcgat accccagaac tgacggttac gcaggatctg accaacggac
     5161 attttcttcg ccgtgctcac ctggtgctgg gcttcctgac cattaataat atagtcgcgt
     5221 tcttcatcgg tcagatgctt ctggtcgcgc ggatgtttat agaaaatcag ccatgccatc
     5281 gcccagataa agctcaatgc accggagatg ataaatgcca tctgccagct gtgcattacg
     5341 attgcccata ccaccagcgg cggcgcaatc atcgcaccaa tcgaagaacc tacgttaaag
     5401 tagcctactg cgatggaacg ctctttcgcc gggaaccatt cggagctggc tttcagaccc
     5461 gccgggatca tcgctgcttc cgcggcaccg accgcaccac gagcaacagc caggccaccc
     5521 cagctacctg ccagcgcggt tgcaccacag aacacggccc acagcacagc aaacattgca
     5581 taaccgattt tcgtacccag cacatccagt acataacctg ctaccggttg catgaccgta
     5641 taagcagcag aataggctgc gatgatatag gaatactgtt gggtggagat gtttaactct
     5701 tccatcagag ttggcgcagc tgccgccaca gtgttacgcg tcaggtaacc aagcacggtg
     5761 ccgagcgtca ccagtgcgat catataccaa cgtaaccctt taattttacg catgtaaaac
     5821 ctcatcgttt tgttatatcc gcaggaagcg cggccatcat cccgccagag gctgaagatg
     5881 ttagttacga ccgggtaacc gaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt
     5941 tcgtgctaat tcggcttccg taccggttaa cccgattcat ctaacaaatg aatgaatcgg
     6001 caatttgcat tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaact
     6061 ttaaaaggtg agagccatca caaatgtggg aatatttgta gggacattac ctgacgacag
     6121 caaggccagt actggcgcgg cctgcagcga gatttaccac tttgagagta atttttttaa
     6181 ctacgtttat tgatctaact cacgaaaata tcttcggact ctggaaattg gtgtgataac
     6241 tttgtcagca tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac
     6301 tccgtttatg actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga
     6361 ctacgcaaaa gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc
     6421 ggaagactat cgttttaaaa acctgtatga catctggctg aaaggcgatc actacaaatg
     6481 gcgcgctatg cgtaccaacg gtgtggccga gcgtctgtgt accggtgatg cgtctgaccg
     6541 tgaaaaattt gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca
     6601 ctggacgcac ctcgaactgc gccgtccgtt tggtatcact ggcaaattgc tttctccgtc
     6661 aactgccgat gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctccgc
     6721 gcgcggcatc atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga
     6781 ttctctggag catcacgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc
     6841 gagctggcgt ccggacaaag ccttcaacat cgaacaggcg acctttaacg actacatggc
     6901 gaagctgggc gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgccct
     6961 gaccaaacgt ctggatcact tcgccgctca cggctgtaaa gtgtctgacc acgcgctgga
     7021 tgtggtgatg tttgctgaag cgaacgaagc ggaactggac agcattctgg cgcgccgtct
     7081 ggctggcgaa accctgagcg agcacgaagt ggcgcagttc aaaactgcgg tactggtgtt
     7141 ccttggtgcc gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg
     7201 taataacaac ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa
     7261 cgaccgtccg atggcggaag agctgtctaa gttgctgagc aagcagaatg aagaaaacct
     7321 gctgccgaaa accattctct actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat
     7381 gatcggtaac ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg
     7441 gtttaacgat cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggttt
     7501 gctgagccgc tttgtcggta tgctaactga cagccgtagc ttcctgtcat acacccgtca
     7561 cgaatacttc cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc
     7621 accggcggac atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg
     7681 tgactacttc gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc
     7741 catgcgctgg ataacgtcgc ggtcgcttta gcggatttgg ctgaaggcac agaagtcagt
     7801 gtcgataacc aaactgttag gctgcgccag gatgttgctc gtggacataa atttgcgtta
     7861 acgaatatcg caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg
     7921 gcggatattg cggcggggga acacgttcac gcccacaata cgcgcacgaa tctgagcgat
     7981 ctggatcagt atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcgt
     8041 gaagtgcaga tctatcgtcg cgctaacggc gatgtcggag tgcgtaatga gctgtggatc
     8101 ctgccaaccg tgggctgtgt caacggcatc gcgcggcaga tccaaaatcg tttcctgaaa
     8161 gagaccaaca acgccgaagg tactgacggc gtgttcctct tcagccacac ctacggctgc
     8221 tcacagctgg gcgacgatca cattaatacc cgcaccatgc tgcaaaacat ggtgcgccac
     8281 ccgaacgcag gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca ggttgccgca
     8341 ttccgtgaaa cgctgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag
     8401 caggatgatg agatcgaagc cggaatcgag catttgcatc agctgtataa cgtgatgcgc
     8461 aacgataaac gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt
     8521 tctgacggtc tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg
     8581 attgctaacg gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag
     8641 ttgctgatgg accattgccg cgacgaagca acgtttgaaa aactggtcac catggtcaac
     8701 gacttcaaac agtactttat tgcccatgat cagccgatct atgaaaaccc atcgccgggg
     8761 aacaaagcgg gcggtatcac cacgctggaa gacaaatcac ttggctgtac ccagaaagcg
     8821 ggttccagcg tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccagggctg
     8881 aacttgttaa gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc
     8941 tgccatatgg tgctgttcag tactggtcgt ggcacgccgt atggtggatt tgtgccgacg
     9001 gtgaaaatcg ccaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac
     9061 gcgggtcagt tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgacacc
     9121 atcgttgagt ttgccaacgg taagcaaacc tgtaacgagc gtaacgactt ccgcgaactg
     9181 gcgatcttca aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc
     9241 gtttcataat gtgaagcgcc gtttgctttt ccatacctgc taaggattta tcatgaccgc
     9301 gtattggctg gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt
     9361 caatcgtgac gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt
     9421 acattttttt cttctgggta cctatcccgc cggtgccagt gccatcctta atgccgttcg
     9481 tacattgatt accttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac
     9541 tggcggaatt ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac
     9601 gattgtcagt acctgggcgc tattccgctg taaagggctg accatgcgct gcgtaatgtg
     9661 gttttcaacg tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacaat
     9721 gattgagggt agttttctgc ttatgaatgg cctgaatatc attcgtttct ggcggatgca
     9781 gaaaagggga attgatccgt ttaaagtaga gaaaacccct cccgccgtag acgaaagggg
     9841 ttaaacaatt aattacgcag ggcgctattt gccagacgat cgtcttccgc ctggcaagct
     9901 gccgcagtga acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat
     9961 acgccgataa taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac
    10021 atattacagg ccagtgggat cagcagcaga gaaccccccg ccacgccgga tgcgccacag
    10081 gcacacagag aagccactac gctcaacagc agcgctgtgg gcagatcgac cggaataccc
    10141 agcgtattaa ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg
    10201 atggtggctc ccagcggaat agaaacggaa taggtatcgc gatccagatt cagcttttca
    10261 cacagcgcca tattcaccgg aatgttcgct gcagagctgc gggtgaagaa ggcatacaca
    10321 ccgctttcgc gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac
    10381 accagcaatg ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc
    10441 agttgcgcgt agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccagc
    10501 ccaaaaatac caatcggtgc gaagcgaatg accagtttca ccataaaggt aacggcattc
    10561 gacatatcgt taaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaagccgagg
    10621 ccgatcgccc acaccagaat cccgatgtag ttacctttca gcagcgcgtc gatggggttg
    10681 gaaaccatgc tcattaccag cccgcgcatc acttcgacaa tgcctgacgg cggcgaaata
    10741 tcacccgcgc tactggataa atgcagggta gaagggaagg caaagctgaa gactactgcg
    10801 gccagagcag cagagaaggt gcccagtaga tagaggaaca aaatagggcg gatattggtt
    10861 ttctgcccgt gctggtggtt agcaatagat gccatcacca gcatcaacac caggatgggg
    10921 gcaacggctt tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg
    10981 ggttttgaga tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc
    11041 aggctgccat gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga
    11101 tcctttcatt gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt
    11161 aacaaagtgt ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc
    11221 tggattttaa agagaggtca tattttgcaa cttaatggtt acatttattt gacataaaaa
    11281 taaagccaac gtgcggcttc cagactgatg acaacatcaa aaaaggactc ggtcagtccc
    11341 ctcgccccgt ttggggagag ggttagggtg aggggaacag gccagcatcg gtgcgaaaaa
    11401 ttaccctcac cccgaccctc tccctggaag ggagagggag aaaacggtgc tcatgttaat
    11461 gacagggtac acggcttagg cagattaaaa attatccacc ccgctgctta tcatgccgat
    11521 aattcaccca ggcgttgata ataaacgtca tcaccagaat gccaaacacc acgcccagcg
    11581 agacggcgat tggaatatgg tagaagtcga caatcagcat cttgataccg ataaacacca
    11641 gaatcaccgc caggccatat ttgagcatcg agaaacgctc tgctacgccc gccagcagga
    11701 aatacatcgc acgcaggccg aggatcgcaa acaggtttga ggtcagcaca atgaacgggt
    11761 cagtggtcac ggcgaagata gccggaatgc tatccacggc gaaaatcacg tcgctcaatt
    11821 ccaccagaat cagtaccagc atcagcggtg tggcgtacag caatccattc ttacgcacaa
    11881 agaaatgctc gttgtcgatg gtgtcggtca tgcgcaaatg accgcgtagc cagcgcacca
    11941 gcggcttgtc gccaatgcct gattcatctt catgggcgag cgccatcttc acgccggtaa
    12001 acagcaggaa ggcaccgaag atatacagga tccagtcgaa ctgcgaaatt agccagctgc
    12061 cagtgaagat catgatggta cgcagaacaa tcgccccgag cacgccatac accagcacgc
    12121 ggcgttgtaa tgccgccgga acagagaaat agctgaacaa catcagccag acaaagacgt
    12181 tatcgaccgc cagcgatttc tcaatcagat aacctgtgag aaaggccagt gcctgtggat
    12241 cggcaacggc gcgaccttcg gtttgcacca gataccacca gaaagcggcg ttaaacagta
    12301 acgacagcgt cacccagacc agcgaccagg ccgcagcctg tttcatggtc atggcatgtg
    12361 ccccacgacg cccctgcaac aacaggtcga tagccagcat aatggcgaca acaacagcga
    12421 atccgcccca tagcaacggc gtgccgacag tattcataga agttccttac acataaaaaa
    12481 acggccaacg tcggaagacg tcagccgctg ctttttatgc atagacctcg ccttccggca
    12541 aggtctcact tacaacaaaa aaggaatacg tctgcgtatt cctttcgggt tgcccggtga
    12601 ccggatgtgg tttttcacac atcgtaatga cgatcgaccg gcaatgaagt tactcccctt
    12661 tgcaggtaac aaagtatgac agaccattca gccagtcaat ggtctgttac atacctttta
    12721 cactgtttta ctttggcaat tatagtttta cgctatctgc cggaaaaatc accccagtct
    12781 ggcggcggat ctcggtcagc agtttggcgg tgatgcgact gaccgccagc cctggatgat
    12841 ccaccagatg ctcatccacc agggtagcga acagctctgc ttcgtagagc atggtattaa
    12901 tatgctgcgg ctgggtgaga tcctgcattt ggctgccacg cggcacgaag cacactttct
    12961 ggcattcaga cagtttttca atcaccagcg atcctgcttc gccctgaatc tcgctcgcca
    13021 ggacagaatc actgactttt gagtgctgca aggtgacgct gaaatcaccg taatccatca
    13081 ccaccacgcc ctgggcgtca acgccgcttg ccagcaaact ggcggttgcc tgcacgcttt
    13141 tcggctcacc aaataacgcc accgccgacg ccagacagta aaaaccgata tccataattg
    13201 aaccgttaga gaatgccgga ttaaaggtgt tgggattctc accatccagg taacgttgat
    13261 accgcgagga atactggcaa tagttgaaaa agactttccg cagtttgccg actttcggca
    13321 gcgcctggcg caacaaatga aagttcggca ggcaggcggt tttaaatgcc tcaaacagca
    13381 ccacctgatt ttcccgcgca caggcaatgg cggcatccac ttccgccaga ttcgacgcca
    13441 gcggtttctc gcaaatcaca ttaattttat ggctaaggaa aagttgtgtc tgggaaaaat
    13501 gcagggaatt cgggctggca atatacaccg cgtcaatggc atcgctttcc gccatcgctt
    13561 ccagcgaggt aaacagatgc tcgacagaaa aatcattggc gaagtgctgg gcctgttcaa
    13621 ggctgcggga atatacggcg gttaacttgt atttaccgct ctcatgggcg gcctcgacga
    13681 actggcgagt gatccagttc gtaccaatca cagcgaaacg tatcataacg cgtggatact
    13741 ccataaaggg attctgtcgt caatgtagca cgcctttttc atcttcgacg tgcgcagata
    13801 cgcattattt ctgcgccgga cagccatgct cactaaacgg cccaatcatg accgaaggcc
    13861 cgagatattt cggctggcga tggaggattt tggcgtcaat cacggcgctg acacgagcga
    13921 gtttctcccg cagttgagta taaaaaccgt gcttcatatt gaggtctggc gcgttaaaac
    13981 cgatagcgtc gatgccgtac tgttttgcca gccagatggc gcgttgatta tgaaattctt
    14041 gcgagataat ggtgatatgg ttttcaccaa aaaccttttt ggcacgcact accgaatcca
    14101 gcgttgagaa tccggcatag tcgcagaaga tcacttttgc tggtacgcct ttagcgatca
    14161 aagcctgctg catacctgat gcttcatcgt aatttttacg tccgttatcg ccacttacca
    14221 gcagccattt cacttttccg gcgtggtaca acgctgctgc ggtatcaata cgtcgggtaa
    14281 agtagcgatt acccggcctc gcccccagca ataaacccac gttacgcgcc ggaacggcgt
    14341 tgacatcgct ccaggtcagc tgtttgctgg cgttgaccat tacgcgatcg gcgataagga
    14401 tggtcgctcc tgccacaagt aacagtaagc aggcaatctt tagagtccgg cgagaaaaac
    14461 aaatacggag aagaaggcgg gcaaatgcgc gcagcatggt cgttatcctt aacgaaaggg
    14521 ggggaggtcg attttaggcg ctaaagaaag aaaagataac gcctgacatc attttcagat
    14581 tttgtcctgc ccaagcgaca actgcgttag ccacagtcgg gtgtcgaact caagctggtg
    14641 gtactggggt tccatatgac aacacaactg gtaaaacgct ttgttatgct ctttctcctt
    14701 caggtgcgcc agctcgtgca ccacgatcat gcgcaaaaac ggttccggcg cgttgcgaaa
    14761 cacggtagcg acgcggatct ccacctttgc tttcagcttg ccgccctgca cacgagaaac
    14821 agcggtatgt aacccgagcg cgttttttag cacatggatc ttattgtcat acatcacttt
    14881 attgatcggc ggggcattac gcagaaactg atttttcaga tcctgagtat attgccagag
    14941 ggctttatcg gtggcgtagt cgtgtgttcc cggatagcgt tttgccagca catcgcccag
    15001 acgctgttcg ttaatcagtg ttcgcacctg ggaaagtagc tgctcgggat aaccctgaag
    15061 ataagtaaga ttgctcataa ccgcccacga aaaagagaaa aagggtatac tcacgcaccc
    15121 ttttcagggg caatgtcgaa attttatcat tcaggagggc cgatgagcca cttagacaac
    15181 ggtttccgtt cactgacact acaacgtttt ccggcgacgg atgacgttaa cccgctacag
    15241 gcgtgggaag cggcggatga atatttgctg caacagttgg acgacacaga aatccgcggc
    15301 ccggtgttga tcctgaatga tgcctttggt gcgttaagct gcgcgctggc ggaacataag
    15361 ccgtacagca ttggcgactc atacatcagt gaactggcga cgcgcgagaa tttacgcctc
    15421 aacgggattg atgagtcgag cgtgaagttt ctcgacagca ccgccgacta cccgcaacag
    15481 ccgggtgtgg tgctgatcaa agtgccgaaa acactggcat tgctggaaca gcaactgcgt
    15541 gcgctgcgca aagtggtcac gtcggataca cgtattattg ccggtgccaa ggcccgtgac
    15601 attcacactt ccacgctgga actgttcgaa aaagtgctcg gcccgaccac caccacgctg
    15661 gcatggaaaa aagcgcgcct gattaattgc actttcaatg aaccgcagct ggccgatgcg
    15721 ccgcagaccg ttagctggaa gctggaaggt actgactgga ctatccacaa ccatgcgaat
    15781 gtcttctccc gcaccgggct ggatattggc gcacgcttct ttatgcaaca tctgccagag
    15841 aatctcgaag gtgagattgt cgatctcggc tgtggtaacg gcgttattgg cctgacgctg
    15901 cttgataaaa acccgcaggc gaaagtggtg tttgtcgatg aatcaccgat ggcggttgct
    15961 tccagccgtt tgaacgttga aaccaacatg ccagaggcgt tggatcgctg cgagtttatg
    16021 atcaacaacg cgctctccgg agtggagcct ttccgcttta atgctgtgct ctgcaacccg
    16081 ccgtttcatc agcaacatgc gctgaccgat aacgtcgcct gggagatgtt ccaccacgcc
    16141 cgccgctgcc tgaaaatcaa cggcgaactg tatatcgttg ccaaccgtca cctggattac
    16201 ttccataaac tgaagaagat tttcggcaac tgcaccacca tcgccacgaa taataaattt
    16261 gtggtgctga aaacagtgaa gctggggcgt cgtcggtaag attgggagtt acctgccgga
    16321 tgcggcgtta acgccttatc cggcctacgt tcagcacgtt actcccaggc cggataagac
    16381 gcggcaagcg tcgcatccgg caatgtctgc ccagttctgg aatcgcgctt ccataaaaca
    16441 aaaaatccct ctcaccatcc tctacattct cttgtttagc gtttttctac gtttattctt
    16501 ccgtcacaca gataaattca tccgttgcca atctgtcaac ggatgttatc atattgccaa
    16561 tttgtcaacg gacgtgatga agaatgcacc tgataactca aaaagcattg aaagatgctg
    16621 cggaaaaata cccgcaacat aaaacggagt tggtggctct ggggaacacg attgctaagg
    16681 gatatttcaa aaaacctgag tcattaaaag cagtattccc atctctggat aacttcaaat
    16741 atctggataa gcattatgtt ttcaatgttg ggggcaatga attacgtgtt gtagcaatgg
    16801 tcttttttga atcgcaaaag tgctacatac gtgaagttat gacgcataaa gaatacgatt
    16861 tctttaccgc tgttcatcgt actaagggga aaaaatgatt acgattgccg acatcttgca
    16921 agcaggagaa aagctaactg ctgtggcacc ttttctggcg ggtattcaga acgaggaaca
    16981 atacacccag gcgctggaac tggtagatca tctgctgctc aacgatcctg aaaacccctt
    17041 actggatctg gtgtgtgcca aaataaccgc gtgggaagaa tcagcgcccg aatttgcgga
    17101 atttaatgcc atggctcaag ccatgcctgg cggtatagcc gtgattcgta cccttatgga
    17161 tcaatatggt ttaacccttt ccgatctgcc ggaaattggc agtaaatcta tggtgtcacg
    17221 cgttttgagc gggaagagga aattaacgct ggaacacgct aaaaaattgg caacgcgatt
    17281 cggcatttct cccgccttgt ttattgatta acgcgacgtg ccggatgcgt tgcatccgga
    17341 gcttcaaata acgatttaaa tctccagcgc cagccgtgtt ccctgggcaa ttgcccgtcg
    17401 tgcgtccagc tccatagcca catcgcagcc gccaattaaa tgcacggttt tcccgctatc
    17461 aatcagcggc tgcgccagcg cgcggtttgg ctcttgccct gcgcagatca ccacattgtc
    17521 cactgctaat acctgcgttt cgccgttgat catcacatgc agcccgtcat cgtcaatctt
    17581 ctgataactt acgcctggga tcattttcac accccgcgag agcagggtgg tgcgatggat
    17641 ccagccggtg gttttgccta acccctgtcc tggtttgctg gctttgcgct ggagcatcac
    17701 aatctgccgt gggctacggg ggatctgcat tccctgcggg cttaagccac cagcctgttg
    17761 taggctactg tcgatccccc attcattaca gaacccggcg atattctggc tggtggattc
    17821 gcccggctga cttaaataca tcgccgtgtc aaagccaatc ccaccacaac cgatgatggc
    17881 aactttgttg cccaccggcg ctttgtcgcg cagtacatcg agataactca ataccttcag
    17941 atgatcgatc ccgtcgatgg gcggagtgcg cggcacgatc ccactggcga ggatcgtttc
    18001 atcgaaagcc tgtaactgat ccgccgtcac ggtgtgattg agttttagcg tcacgcccgt
    18061 cacttcgatc atccggtggt aatagcgcag cgtttcgtaa aactcctctt tgccggggat
    18121 ctgtttggcg atattaaact gcccgccgat ctcgctgtgg gcatcaaaca atgttacctg
    18181 atgcccacgc gccgccgcgt taatggcaaa cgccagccca gcaggtcccg caccgaccac
    18241 cgccagattt tttttctgca cggcaggaag gattggcatt ttggtttcgt ggcaggcgcg
    18301 aggattcacc aggcacgagg tgactttgcc aacgaagatc tgatcgaggc aggcctgatt
    18361 gcagccaata caagtgttga tctcatcggc tcgccctgat tgcgcttttg acagcagttc
    18421 cgcatcagca agaaatggtc gcgccatcga taccatatcg gcatcgccgc gcgagagaat
    18481 atcctcggca acctgcggat cgttaatccg gttggtggtc accagcggca gcgagacgtg
    18541 acctttcagt ttgcgcgtga cccagctaaa tgcaccgcgc ggcacgggcg tggcgatggt
    18601 cggaatacgc gcttcatgcc agccaatgcc ggtgttgata atggtcgcgc ccgccgcttc
    18661 aatggcctgc gccagttcta ccgtttcggc aaaagtccca ccgccttcga ccaggtcgag
    18721 catcgacagt cggtagataa taatgaagtc gttgccaacg cgttcgcgta ccgcactcac
    18781 cacttcaacg gcaaaccgca tccggttgtg gtaatcgccg ccccactggt cactgcgctg
    18841 attggtgcgc agcgtcagaa attcgttgat caaataccct tcggaaccca tcacctctac
    18901 accgtcgtat cctgcctccc gtgccagttg cgcgcagtgg gcgaaattgt cgatcagttg
    18961 caggatctct tcatgggtga gttcgtgagg aacgaaacgg ttgatggggg cctgcaatgc
    19021 ggagggagcg accagatgcg gttggtagct gtagcgcccg gtatgcaaaa tttgcagggc
    19081 tattttgccg ccttcctgat gtaccgcttc ggtaatggtg cgatggtgtg ggatctggct
    19141 ggcgtcgttg agcattgcgc caccttccat gccaacgcct gttaaatctg gtgcaatgcc
    19201 gccgctaaca atcagcgcca cgccgtgacg ggcgcgttcg gcataaaacg cagccagccg
    19261 ttcagcaccg tccgggtatt cctccagccc ggtgtgcatt gaacccatca acacgcggtt
    19321 ttttaacgtg gtaaaaccta aatccagcgg ggcgaacagc gacgggtagc tcataaaatt
    19381 gtccagtatg taaaataatt gttatgtggt cggatgagtt ctaatttagc cgtcgccgga
    19441 gaaaagggaa aaggggagtg tggggtttgt gatgggattc aaaaataata cgcccattgg
    19501 aaatgccgga tgcgacgctt gtcgcgtttt atccaggtta cggaattgca catgtagggc
    19561 ggataaggcg tccacgccgc atccgccagt ggcgcggtgc agatgccgga tgcgacgctt
    19621 gtcgcgtctt atccgaccta cgggaacgca catgtagggc ggataaggcg ttcacgccgc
    19681 atccgccagt ggcgcggtgc agttgccgga tgcaacgctt gccgcgtttt atccggccag
    19741 gaggattgca catgtagggc ggataaggcg tttacgccgc atccgccagc agttgcaaat
    19801 tattgcttac ggaaaaaatc gttatacaac ataaacaaat gcgcggcact ccaggagaaa
    19861 ttcggtgccc cttgctgcgc gccagtgagc gggttgtaat tctcctgaat tgggccatcg
    19921 gtggttaatc ctttggcgtg ccggaagaac gtatccgcca gtttcagggc atcatcgcga
    19981 taaccgtaac gctccatccc tttcagacca aaccagaact gatccaccca tacgcgcccg
    20041 cgccagtaga tatccgcgcc aaaagccggg ttggttaacg ccgccgttcc cagcgggaca
    20101 aaggtgttga actctttagg atcgagcatc accttcacca ccgcgtcggc attggcctgc
    20161 gttgccgcac cgttaaacag cggcgaccag ccttccggcc ctttaccgcg ctcaacaatc
    20221 ggtttgcccg cgcagccgtt cgccagcggt ttatcttcaa tacgcacgtc atagtagaac
    20281 tgggtagtcg ggtcgaacat acaggtgttg atgtagtccg cgagctgctg tgccaactgg
    20341 cgatagcgtt tggcctcttc tggcttaccg aggatggttg ccatctccgc cagataatgg
    20401 ttatcgctgt acatatagct ggcctgatcc accgactcct gcaatagcga gtagcccagc
    20461 aacgttccgt cctgactgcg gttttcggcg aatttcaccg tccagtcgct acgtttgccg
    20521 ccattagcga catatttatc caactgttct ttgtcgataa acccaaagac ggcggcatca
    20581 tcacgccccg attcccacga tgcagcaacc tgtgccggaa tttccagact gtcgtactgg
    20641 cctttctcca ccacgcgggc gtagttgttc agcccagact gcgtctcttc tttatcgcct
    20701 tttttcaccg taaacagcat ctcgccgctc tcagtgttgt gggctttgtc gcgggtcgcg
    20761 ccatattccg gcacgccgtt gccgttgtga tcgcggttac gtaaccacca gtcgtgatag
    20821 gccaccagtt tcgggtacat ctctgccacc caggttttat cttgggtgac gttatacact
    20881 tccatcaccg accaggcggc aaggctgggt ttggtattat gttcgttcca gttgccgcca
    20941 tcgccaccac gctcggggct aaggttccac gctatcaggt cggggacaaa gcccacatcc
    21001 tgcggacgca cgctatcgcc aggctggatc tgccaggaga agaccgcgcg gatattctct
    21061 ttggcgatgt ccggattgaa atgcgccatc gcaaacgcct gcttccaggt atcccacggc
    21121 caggtctgat taccggagaa ccagtgcccg gtcaccgacg gtgtaacggt gttaaatttc
    21181 accgcaccgc ccggtgagcg ccagttaccg ttgagcgttt cgatcgcttt cactgcgacg
    21241 cgcgtctgtt ccggcgtcgc atccggattg gttaaccctt tcttcaggta ttcttcccag
    21301 cgttgctgcg aggcggtgag ataaaacgcc ggacgcgcca gaatatcgcg gatctgcatt
    21361 tgctctttgc taacttcctg agaggtcagc agatgggaat aggtggtata gagcgtcgtc
    21421 gaaccgttga tatgtgcctt actggtaaag cgattgccgt tgatttcagt ctgcaccggc
    21481 agggatttat gcacctgata ttccgattcg ccggaggtca gcagatccca ggtggcgcgc
    21541 actttgccaa aggtgacttt caggccatca cgggtggcgc tgattttgcg ctgatagtca
    21601 gggtactcgc cagcaatggt tttatcggaa agcggtttcc cttctttcgc ttccagtttt
    21661 tccagcagtt cgccgtccca caccagatcc agcggtttat tgctggtgat tttggtttcc
    21721 agtagtgacg tgcgcggcgt ggcgaagcgc agagtcattt cgacttgcac atcttttgct
    21781 gtcagttttt gcaccagcgc accgggaata ctgtatgcct ccagcgtaaa gtcgactttc
    21841 ttgccgtcct gccagacggt caggcggtcg aaattgctgg ccataaagtt gatgtactct
    21901 tccgtcagca gcgcaacgcc cggaaagccg cccatggtgt tagggccgtc tggcaacaga
    21961 tgaccatgcc aggctccgag atcgaaaaac ggattaaaac gctggtgatc gtcgtaatcg
    22021 taatccttca tgtactgcgg cgcgccagta cggttaatca cgtttttata attgtcggcg
    22081 ttagcagcat gggcggaaaa acttatcagc agagcgcagg ttactggcgt taaaatagtt
    22141 ttgattttca tgaaattaca gtccttatta ccagtagaaa tattgcatca ggaagatttg
    22201 gtcgctggga tcgttagagg catttttcat gtagaagcgg ctatcaaaag ccgttgcgaa
    22261 acgtccgccg ccatattcgt accagatacc aaactgcatg aaagaggtgg tttcatcgtc
    22321 agcgtggtca gggcgatgtt tggcgtaggt gtaagcggtg gagagataaa cgccctttaa
    22381 tgcttcatac atggcgctca ccatcatccc actttcactt cccggtacgt ccagcccgtc
    22441 ggcgcgtccg gtatggtgcc aggcgcgggc ggcgaagtta ggggtaagca ggccggtgag
    22501 atagagttgt ccggtgccgt cggtaatttc cagaccgttc atccaggtga tgccttcggc
    22561 aaggtcatac tggatgtaac cgttcatcat tgccggatag gtgtatttgt cgtcatagcg
    22621 atcgtatttg ccaaaatgta gccaggcttt gctttcgtca tgatgtccgg caggtgtggc
    22681 ggtgacgcta taacgaaagt tgccgcttaa gttctgcacc ttcagcgcgg tcataatgtc
    22741 gcgggtattg ggaatgacgt agcccagatc gggggtgaag tcgccccacc attgcaggtc
    22801 atccagtgag gaatcctgac gcagactgag catgatttcg gtgccatcat tggtacgata
    22861 accgccatag aaacggttaa ttccgccctc gaaaccaccc cagctatggt ctggcaccca
    22921 ggcattgccg tcgtggtcgg cctgcacggt ccagccttcg ccgtaaatca ggccgaacca
    22981 ggagccgtac ttcatctcca ggccaccttc aatataggta ccgtcgctgt atttatgttt
    23041 attctcaccc tctaaatcca tatatccacc aacccctaat tcgccgtaaa aacgaaatga
    23101 aggagaagat gtcgaggtaa ttgcgggtgt ttttgtgtcg tcatgttctg ccggacgtgc
    23161 ttcttctcct gccgcagaaa aggcgtagca aaacggcaga gcaagtaatg ctctgcatgg
    23221 tgcagtgatt aatttcattg tgttaatcca tgagctaaag aaaaagtaca gccttttatc
    23281 gttgtaatta taaaaatata aaggcagaaa ctattttccc tctcctcatc caggaagagg
    23341 gaaaatgcct gctcattttt tattattctg cttaatcaac attgctggct ggcgtcgctt
    23401 caatatggcg ctcttcagcc gttaatccct ttatatattt actgtatttc caccacgcaa
    23461 agccaaggaa gataacaata ccgccgacgt tataaaaaat gatggtcaga atattcgcgc
    23521 cagtcgggaa tgtcgaagcg acaaacccta cggcaaatat cgcaatcagc attgaaacaa
    23581 cgataatacc ggtgcggcgg gagcccatac ggaaatcgcg tggcaggtga tctaatttgg
    23641 cgcgtaaatt caggtaagcc agcatgatga ataacggcgg aagcatggac gctgcggcgg
    23701 tcatattaat aatagtattc atcagatcct gcacggtatt ggaaccgagc atcgggataa
    23761 tcatcagcgg gatgacgatc aggaactgga tccacgctgc gcgcgccgga acgccgtttt
    23821 cgttcagttc gacggttttc ttaccaaaga tgccttccgg gatttcggag aagaaaattt
    23881 tcaccggcgt tgcggtccac atcagcaggg aaccgaacat cgcggtaaag gacaccagac
    23941 cgacaaagcg attcatcaac gcttccggta gaccaaaata cgccgccatg ccgtggaata
    24001 cctgcaccga tccgccggta aatttcaact ctttgctgct gacgaagacg ttaatcagca
    24061 ccgaggagac ggaatacagt acgccgataa aaatcccggc gaggatgatc actttaacga
    24121 acgatttcga accgcctttg acgtcgttaa cgtacacagc gacggactcc gcgccacctg
    24181 cggccataaa gatccaggtg gtaacgccga ggaacgccca gttgaagttc gggatcatcg
    24241 cgtcaacggt gatggcgtca gcaggctgta cgccgccaac cagcgccgta cctgccagta
    24301 aaatgtagga gagcgttaac agcagcatca gcgttgaagt gacggaggta attggcccca
    24361 acattttcgc cccgttggtg gaaacccagg tggagaaggc gaacagcacc atactgataa
    24421 tggtggtggc aaccggcgtc ataatatatt cgtatccgag gaaggcatac gaagcataag
    24481 caataacgcg cggcaacagt gaggtgaaaa agaacaggtt tacgaaccag taggtatagg
    24541 cagtaataaa tgcccaacgt ccgcccagcg aactttttac ccacgcgtag acaccggctt
    24601 ctgagttttt atttaacgaa acaaattctg cgatgatcag acagaaggga ataaaataaa
    24661 aaatcgtcgc gaggaaaaac atcggtgccg aggccagtcc aagctcaata ttattattga
    24721 taacgttgtt aaagctgtaa acggcggcaa aagtcagcga gagcaagccg aatttgccga
    24781 ttgtattacg tttggtatca gacataacac atctccgaaa atgagtaggg tagcggtggc
    24841 ggcaaactcc ttttgccgcc gtagttgtta tttgttatgg aaataaccat cttcgatggt
    24901 gactttgaga accacttttt tgaccgcgtt attgcagata aaccgatacg cttcatggat
    24961 atcgcaaata acgatttgcc cttcgtggac ctcaacggtt tctccgcagc cttttaaata
    25021 ttcacggtca gtttcatcac gataatattc cactacctgt aatgtctctt tcggcgcata
    25081 ttcaattttt tgctgccctt gcaggtagta atgcacttca aaatagcgtc gatgcccggt
    25141 aaatagcgca tcggtcgcgg agtctgtctc cacgcggtaa gtcaatgagt caccgatgga
    25201 gtgggcgacg ccaggctgaa tgttgtcgat attttcaatc gcttcaacgc agcgttgcca
    25261 cttcttgcca gaggcgtaaa tctggcggaa ctgttctaag ttatcgatga tcctcattgc
    25321 ttattctcgc tgtgcaaatt cgtggaaaag aaccctgcgc cgaactcata cgacgccagg
    25381 ctttgcgcgg tagcttctcc gccagaaacc ggcagcaacg taaagccgta gctgaagtca
    25441 cggaaccaga cgcgccagga gtccagcacc tcgctgcccc aggagttgga gccgaggcca
    25501 agcagctggt gatcgaggtt cagggtgatg tcatcactgc gctgcagctc gttacagtgc
    25561 tgggcagcgt ggatgttttc ctgggtatag tgccaggcgc tgaagttaat tgggcgctgc
    25621 ggaaccacca gcagaccgtt accgtggcgg ttagtcagtg ccgtccagcg gacatgctga
    25681 cggttaccgt tgttctgcgg gaagggatag ttctcgaaca tggcatcgac ggtgctgcgc
    25741 cagatatcga tgatgttagc ctgctggctg tcggcgtagt tttcgcccgg tccacgaccg
    25801 taatacgcca cctgatcgta ttcgccgtta attcccatgg tgaaaccgat gcacggaatg
    25861 atgtgcggat agtcgccgta acgctcgccg gaaagcgcca cgttaacctg gccatcggca
    25921 gcgatgcgcc agatgtaggt gcagcgcatc ccgaagtcaa acaccggcgg ggcaataact
    25981 gtgcggctga tgatcagcac ttcaccatcg ctctgttcta cggcaaagtc gcgcagatgt
    26041 tcctgcatga tctgcaaatg attcggttgc cacagccctt cgtactcctg cttgtggttg
    26101 tcgatcatcg gcttgaagaa gttgatcttt ggctcgcgag tcagcagcga ttcgccattc
    26161 acctgccagg atgtcggttt gccactcatt tttgagaagg tgatcgcgaa gttgtagccg
    26221 cgaacggtgc agctcaaacg atcgtcttcc agcgtcagcg gacgcgcatt atttggtgcg
    26281 aaaggcactg gctgcgcggt gttttccttc agcgggaact gataagtggc gatagggtgt
    26341 ccggcttcgc tgtagcgggt gcgggaatct ttggtcaccg taatgttgag gaacgcttcg
    26401 cgggcgtcca gctgcggcag cgtgatctgc aagggggctt cgctgttcgg cgcaacgtcg
    26461 cgcagtttaa tctgctgcgt cgcgagcgtt tcaccttcgg cgcgcacctc tgcgtgcagg
    26521 gtgtagtcat caagcgtggt aaaccacagt ttattttcga ctttcaactc gccgcgagtc
    26581 agatcccgcg cgtggatttt taccggcgcg ataacctgtt tgtactcttt caggcccggt
    26641 cccggcgtct gatcggaata gatcaaacca tcaagacaga agttatagtt gttgggatag
    26701 tcgccgtagt cgccgccgaa tttataccag acattgccgt ggtcgtcctg tgcctggatc
    26761 ccgtggtcgc accactccca gacataatga ccctgaatgc aatcgtgctt atagaagacg
    26821 ttctggtact ccgtcagccc gcccggtccg ttccccatcg catgagcata ttcacagatg
    26881 atgcgcggct tcggatgcgg gtattcacca aactcattca tcagcggcac gcgggtgtac
    26941 atggtggaaa taatatcgac cacttcagca tcgcgatctt cttcgtaatg caccagtcgc
    27001 gtgtcatcca gcgctttcgc cgcatggtac atcgcgcgga tgttacagcc atagccggat
    27061 tcattgccca gcgaccagat gatgatcgac ggatggtttt tctgcgcgtg gatatggcga
    27121 acaatgcgct cgacgtagac tttttcccac tgcggatcgt cggtaatacg gctaatatcg
    27181 ccgacattag caaagccgtg cgattcgacg tcggtttccg ccatcacaaa caggccgtag
    27241 atatcacaca gttcgtaaaa acgcggatcg ttcgggtagt gagcggtacg cacggagttg
    27301 atattgtgct gcttcatcaa ctggagatct ttctcgacgc gatccattcc aacggcgcgg
    27361 cctttgcgat gatcgttgtc gtgacggttg acgccgtgca gcatcacata acggttattg
    27421 atccagaaca gaccgtcgcg cactttgata tcacggaagc caacgcgttg tggcaccact
    27481 tccagaacgt tgccgttggc gtctttcagc gtcatgacca gatggtaaag ataaggggat
    27541 tctgctgacc attgctgcgg ctgttcgaca gtaaaagcaa agctggcgct ggtcagtttt
    27601 tcaattgcca aatgatcaat ggcgctgctg tgcaccacgc gttcgccatc aaacagggta
    27661 tattccagcg tcgtgacgac aggggaggcg gcgagatttt ccagcaccac ttcgcaggaa
    27721 agcgtggcat cgcaataggc ttcgtcaaag tcggtacgca cagtgaaatc gttaatatgc
    27781 gttaggtgtt ttccgaccag ataaacatcg cggaagatcc ccgctgacca ccacatatcc
    27841 tggtcttcca cgtaggtaga gtccgcccac tgcatcacgc gcacacacaa caggttgtcg
    27901 ccggttttaa ccatcgcgct gatgtcaaac tctgcggtca ggcgactgcc cttgctgaaa
    27961 cccacatact gaccgttaac atagacttca aaataggttt cgacgccgtc aaatttaatc
    28021 agcgtctgtt taccctgcca gccgtcgctg agggtgaaaa tacgttgata ggcaccggtt
    28081 gggttatcgc tggggacaaa cggcacatcg atggggaacg gaaaaccttc gtcggtatat
    28141 tgcagtttgc cgtgaccttc catttgccac atggcgggga cggtaatatg cccccagtca
    28201 gccattaact cagaggtgaa ggcttctggt acttgcagcg gatggtcaaa aaagtggaaa
    28261 ttccactgac cgcttaaggg cagaaacagg ctgctggttt cgcgggcaaa ggtacgcgct
    28321 tgcgcaacag aatcatatga aaaaaagtac gcacgcggcg caagtcggtt ttcgtgggtg
    28381 agctgaatgt tttcccagcg attcataggg cctcccggta gagagaaatc ggaaccattc
    28441 atggtgatgg tagtttgtgt aattgctggg gagtttagta aaatttttac taaaaagtag
    28501 caggtaaatg catttacttt aactggatca cgaaaataaa cggcagcact gtccggcaaa
    28561 aacgattgcc ggacagtgtg taaggggatt taacgggtcg tgccgcgcag ttttaatttg
    28621 ctgggaacga agactaacag cggcagcgcg cgaccatcgc gggctttttc atacaccagg
    28681 ttaacgccct gacttcccat catctcggaa tggatgcgca cggtggagag cggcggaaag
    28741 gtaaatcgcg cggtggggat atcgttaacg ctgataagcg aaatatcctg tgggatgttc
    28801 aggcctcgtt catgaattgc ccgcagtacg ccgatagcaa tggaatcgga agcaacaaac
    28861 agtgccttcg gatagtcttc ccgcgccagc atttgttttg ccagttcata acccgacgaa
    28921 ctggaaaaac cgccgcgcca gatatcctct tcgcgtacca cttgtttcag tcggccatat
    28981 tccgcaaagg cgacctcacg aatatccgcc ttgccaggct catcttcacc gccaataaaa
    29041 ccaatacgat taacgccctg gttgatatag aagtcgatga tttctttact gatgcgtgcc
    29101 agatcgatat ccaccgcatc gtaaccgctg ccgggttcgt gaaagtcgat aaaacagata
    29161 ttgtcggtca acgcgctggc agcggcgcgc agggcgggcg tgggtttgcc gacaattaaa
    29221 ataccggtga cgtttttaat gtctggtaag ccgctgtgtt cataacagtt ggtgagctcg
    29281 atgcccagct tttcgcactg ggtttcaatg ccgtggcgga tcgccagata gtaaggatcg
    29341 ttgatctcca gctcctgctg gtagctgtag atagccagaa tatggtgttg gttgactgca
    29401 cctgtctgga gtttacgggc actactggtc ttgtactcca gcttttcggc gatctcgaga
    29461 atgcgatgtt tcgtctcttc tttcacattc aatgtcggat cgtcatttaa gaccctggat
    29521 actgtcgcca gggatacgcc agcttcgatt gcgatgtctt ttagtgttgc catgtttacc
    29581 ttttttactt tccctgatgt gcgcccccat tgtagaggaa gtgcttatcg ggtcacctgt
    29641 tttcagtaaa taaagcgcgt ttctttgacg ctgtaacggt aacacagcgg ctcacgaatg
    29701 tgatcacgcc tgcattctcc atttttagcc tacaggattg agaaaaactt ttgctatagt
    29761 ttagtaaaat tttaccctaa gggaggaagc aatggaaacc gtggcttacg ctgattttgc
    29821 acgtctggaa atgcgcgtcg gaaagattgt ggaagtgaaa cgccatgaaa acgccgacaa
    29881 gctgtacatc gtacaggttg atgtggggca aaaaacgctg caaaccgtga ccagcctggt
    29941 gccgtactac agcgaagaag agctgatggg gaaaacggtg gtggtcttat gcaatctgca
    30001 aaaagcgaaa atgcgtggtg aaacgtcgga atgcatgctg ttgtgtgcgg aaaccgatga
    30061 cggcagtgaa agcgtgctgt taacgccgga gcggatgatg cctgcgggcg tacgcgtcgt
    30121 ataaaaagat cggatggcga cgtcgtatcg ccatccgatt tgatattacg cttcttcgac
    30181 acttactcgc atggccgcca gcgccttacg cgccgctttg atcaccagtt cacactgttc
    30241 aatggtcagt gtcagtggcg gttcaatgcg gatcgttttg gcgttattga gcgttccggc
    30301 caccagtacg cgctggcgga acatctcgct ggcaaagtta tagccgattt cgttatcaac
    30361 aaactcaatc gccatcaaca tccctttacc acgcgcttcc tgtaccagat cgggatattc
    30421 ccgcgccagt tgacggaaac cgtccagcaa catatcgcct ttttgctcag cctgagccgg
    30481 taagttctgc tccagcaaca cattgatggt cgccagcgcc gccgcacagg ccagcgggtt
    30541 gccgccaaag gtggtggtat gcaggaatgg gttgtcgaac agaactgaaa acacctcttc
    30601 agtggcgatg gtcgcgccaa tcggcatcac gccgccgccg agcgctttgg caaggcagag
    30661 gatatccggc tgtacgttct catgctcgca ggcgaacatc ttgcccgtgc gccccatgcc
    30721 cgtttgtact tcatcgagga tcatcagtgc gccgaactca tcgcatagct tacgtacggc
    30781 ggtgagatag cccggcggcg gcagaattac gccaccttca ccctgaatcg gttcgaggat
    30841 caccgcagcc acatcatcac cggttttttt gcactcgtta agagccgtgc gcatggcttc
    30901 gatattgcca aacggcacat gacggaagcc cggcagtaac ggcataaacg gtttgcggaa
    30961 ggtcgatttc gccgtggccg acagcgcgcc aagtgattta ccgtggaacg cgccgctggt
    31021 ggcaataaaa gtaaacttgc cgcgcggtga ctggtaagct ttcgccagct tcagcgctgc
    31081 ttcaacggac tcggtgccgc tattacagaa gaagctgtat ttcagtttac cgggcgttag
    31141 cgcagcaagg gttttcgcca acatcgcccg taacggatcg agcagctcct ggctgtgcag
    31201 cggttgtttc gcaagttgat tctgtacggc ggaaaccaca actggattac ggtgccccac
    31261 gttgaaaatt ccaaaacctc ccaggcagtc gataaactcc tggccctggg tgtcgacaag
    31321 cgtatttaaa cttcccgctt gccactctac ggctccgtaa tccccgccgg cggtaacaga
    31381 tttgcgatac tctaaaaacc ccggattgac atgctctttg aagtattcaa tcacctctcg
    31441 gttaagtgct ttcatctcct catgatccag cgttcgcttc tcaatgagat tcagggcgtg
    31501 ggcgctgcac gctaaagccg atgcgctcga aggtaacctg ttcaaaatat gctccggagg
    31561 ctcgcgtatc acatgatacc gatttaagta ttgcagggat tgcgccactc cggctgcgat
    31621 cgcgaaaatc gggataaaca caaattcggt tatcatttgt gcaatattta atgctatctg
    31681 ttaacatttg ttgttatttt gcacggtttt tgtcgggtat ggatttgatg attgctgcgc
    31741 aaatgcacgt tttcagggca ataattgcct gatttttggg caagataatt aacgttttcg
    31801 tatagtaaca gagtgaaagt attttaaatc aaggaattag atttgtgttt aaatcgcaaa
    31861 ttgcgatcta aatcaaatta atcggttaaa gataaccgca gcggggccga cataaactct
    31921 gacaagaagt taacaaccat ataacctgca caggacgcga acatgtcttc tcatccgtat
    31981 gtcacccagc aaaatacccc gctggcggac gataccactc tgatgtccac taccgatctg
    32041 caaagctata tcactcatgc taatgacact tttgtgcagg tgagcggcta taccttgcaa
    32101 gagttacaag ggcagccgca caacatggtg cgtcacccgg atatgccaaa agcggcgttt
    32161 gcggatatgt ggttcaccct gaaaaaaggg gagccctgga gcggcatcgt gaaaaatcgc
    32221 cgcaaaaatg gtgaccatta ttgggtgcgg gccaatgcgg taccgatggt gcgcgaggga
    32281 aaaatcagtg gctatatgtc gattcgtacc cgggcgacgg atgaagagat cgcggcggtg
    32341 gagccgctgt acaaagcgtt gaacgccgga cgtaccagta agcgtattca taaaggcctg
    32401 gtggtgcgta aaggctggct gggtaaactg ccttcattac cgcttcgctg gcgggcgcgt
    32461 ggagtgatga ccctgatgtt tatcttgctg gcggccatgc tttggtttgt tgctgccccg
    32521 gtggtgacgt atatcctctg tgcgttagtg gtattgttgg caagcgcctg ttttgaatgg
    32581 cagattgtgc gcccgataga aaatgttgcc catcaggcac tgaaggtggc gaccggagaa
    32641 cgtaatagtg ttgagcatct gaatcgcagc gatgagctgg ggctgacatt acgtgcggta
    32701 gggcaacttg gcctgatgtg ccgttggcta attaacgatg tctcaagcca ggtgtccagt
    32761 gtcagaaatg gcagtgagac gctggcgaaa ggcaccgatg aactgaacga acatacccag
    32821 cagacagttg ataacgttca gcaaacggtg gcgaccatga accaaatggc ggcgtcggtg
    32881 aaacagaact ctgccacggc gtcggctgcc gataaactgt caatcactgc cagtaatgcg
    32941 gcagtgcagg gtggggaggc gatgaccacg gtgatcaaga caatggacga tatcgccgac
    33001 agtacccagc gcattggcac cattacttcg ctgattaacg atattgcgtt tcagaccaat
    33061 attctggccc tgaatgcggc ggtggaagcg gcgcgtgccg gcgaacaggg caaaggtttt
    33121 gcagtggtgg caggggaagt gcgtcattta gccagccgca gcgctaatgc tgccaacgat
    33181 attcgcaagc tgattgatgc cagtgctgat aaggtgcaat ccggttcgca gcaggtacac
    33241 gccgccggac ggacgatgga agatattgtg gcacaggtga aaaacgtcac ccagttgatc
    33301 gcccagatta gccattcaac gctggaacag gccgatgggc tttccagcct gacccgtgca
    33361 gtggatgagc ttaacctgat cacccagaaa aatgccgagc tggtggaaga gagtgcgcag
    33421 gtgtcggcga tggtgaaaca ccgcgccagc cgactggaag acgcggtgac ggtactgcat
    33481 taatcggtat gccggatccg gcggttggag cacaatgcct gatgcgatgc tggcgcatct
    33541 tatcaggcct gcggggtgtg tagcgggtgt aggccggata aggcgcgtca agcgtcgcat
    33601 ccggcaattg caccgcgcca ctggcggatg cggcgattaa tccagttgcg taatatcaaa
    33661 agcggcgcgg tcgatcaccg caatgatctt ttttatttgt gcatcactga tatcgctctg
    33721 gttgacgcgt aaatccagca ccgctttaaa attatccagc gcccgcttca tttgcgggtt
    33781 ctggcgcagc gccgcgccaa cgcaacgcgc tttgatgcgt tcttcaatca tctccacctg
    33841 ttcgcggttt tcttccagcc actgcgcgcc ttgttcggtc agcgcaatct gcttcttacc
    33901 tccttcctct tcgcggatgg taatcagcga ctgctcctgc agaaaatcca gcgttgggta
    33961 gatgacgccc gggcttgggg tgtaattccc ctgggttaga ttctcaatcg ctttaatcaa
    34021 ttcgtaaccg tggctgtcat cgcgcgagag aatatccaga atcaccagac gtaattcacc
    34081 gtgaccaaag aaacgttgcc gacgaccgcc gccgcgaccg tggcgaccac cgcagcattg
    34141 accgtgttca tgctggtgac cgtgtccgca gtgctcgtgt tctgacttct cacctttgca
    34201 gcagccctca tggcgtggct ggccttcatg tttacaacac ccttcgtgat gatggctcat
    34261 atcagcctcc ttttgcttaa ttagatatat ctaatttata tctgatatga gcgttcttgc
    34321 aagtgtaaaa atgattttta aacgtggact catctcacac gtaggccgga taaggcgttc
    34381 acgccgcatc cggcgttgag taatgatgcc tgagaagagg cggttcgtct tatcagacta
    34441 tagacatatc agttctacaa atcgcttgca tttatcatga ttaacaatca ttatcatttg
    34501 cgagttttat ttagatatat ctgattacca tcacgaaggc gataacaatg aataacaccc
    34561 cccgctaccc gcagcgcgtt cgcaatgatc tgcgcttccg tgaactgact gtgttacgcg
    34621 ttgagcgcat cagcgccggt tttcagcgca ttgtcctcgg cggcgaggcg ctggacggtt
    34681 ttacatcgcg tggctttgac gatcacagca aactcttctt tcctcaacct gacgctcact
    34741 ttgtgccgcc aacggtaacg gaagagggca tcgtctggcc ggaaggacca cgcccaccgt
    34801 cgcgtgacta tacgccgctg tatgacgaac tacgccatga actggcgatt gatttcttta
    34861 ttcacgacgg tggggtcgcc agcggctggg cgatgcaggc gcaaccgggc gataaactta
    34921 cggtggcagg tccgcgcggt tcgctggtgg tgccggaaga ttacgcgtat cagctgtatg
    34981 tctgcgatga atccggaatg cccgcattgc gccgccgcct ggaaacgttg agcaaacttg
    35041 ccgttaaacc gcaagttagt gcgctggtta gcgtgcggga taacgcctgt caggattatc
    35101 tcgcgcacct tgatggtttt aatatcgaat ggctggcaca tgatgagcag gcggtagatg
    35161 cgcgtctggc gcagatgcaa atccctgccg atgattactt catctggata accggcgaag
    35221 gtaaagtcgt taagaattta agccgccgct ttgaagcgga acagtatgac ccacagcggg
    35281 tacgtgcagc ggcttactgg cacgcaaagt aactgatata taaaaagaaa aaggctgacg
    35341 atttctcgtc agcctttgct atatctggtg gcccctgctg gacttgaacc agcgaccaag
    35401 cgattatgag tcgcctgctc taaccactga gctaaggggc catggtagcg gattataaag
    35461 taactccgtg tcgcaatcca gccattaccg cgcgcctgct gtttttataa acaatgtatt
    35521 ttcaatccgt tatacttttc ctggtgatgt tagaaaggag taaatatggt tgaggatatt
    35581 ttggctccag ggttacgggt cgtgttttgc ggtatcaacc ctgggctttc atccgccggg
    35641 actggttttc cctttgctca tccggcaaat cgcttctgga aggtgatata tcaggccggg
    35701 tttaccgacc gtcagttgaa gccgcaggag gcacagcatc tgctggatta tcgttgtggc
    35761 gtcaccaaac tggtagaccg tccaacggtg caagccaatg aagtttcaaa gcaggagcta
    35821 cacgcaggcg ggcgtaagct gattgaaaaa attgaagatt atcagccgca ggcgttggcg
    35881 attctgggca aacaagcata tgaacaggga ttcagccagc gcggtgcaca gtgggggaaa
    35941 caaacgctca ccattggttc gacgcagatt tgggtgctgc caaatcccag cggtttaagt
    36001 cgcgtttcac tggagaaact ggttgaagcg tatcgcgagc tggaccaggc gctggtagtg
    36061 cgtgggcgat aaaaaacgcc accgaaaagg tggcgtttgt gcagagggcc tagtgccggg
    36121 tgcggcgtaa cgcctgatcc ggcctaccga ttaatcgtcc aggaagctac gcagcacttc
    36181 agaacggctc gggtgacgca gtttgcgcag cgccttcgct tcgatctgac ggatacgttc
    36241 gcgagtaacg tcgaactgtt tacccacttc ttccagcgtg tggtcggtgt tcatatcgat
    36301 accgaaacgc atacgcagaa cttttgcttc acgcgcggtc aggccagcca gcacgtcgtg
    36361 cgttgccgca cgcaggcttt cggtggtcgc agaatccagc ggcagctcga gggtggtatc
    36421 ctcgatgaaa tcccccagat gcgaatcttc atcatcaccg atcggcgttt ccatggagat
    36481 tggctctttg gcgatcttca gcactttgcg gatcttgtct tccggcatca gcatacgttc
    36541 agccagttct tccggcgtcg gttcacggcc catctcttgc agcatctggc gagaaatacg
    36601 gttgagcttg ttgatggtct caatcatatg caccggaata cggatggtgc gcgcctgatc
    36661 cgcgatagag cgggtgatcg cctgacggat ccaccaggtt gcgtaggtgg agaacttgta
    36721 accacggcgg tattcgaatt tatcaaccgc tttcatcaga ccgatgttgc cttcctgaat
    36781 caggtcaagg aactgcaagc cacggttggt gtatttctta gcgatagaaa taaccagacg
    36841 taagttcgct tcaaccatct ctttcttcgc acggcgggct ttcgcttcac cgatggacat
    36901 acgacggttg atatctttaa cctgctcgat ggtcaggccg gtttcttctt caatctgctg
    36961 cagtttttgc agggcgcgat gcacttcttc agagacatcg tgcagttttt ccgaccacgg
    37021 cttgttcatc gcaattgccg cgttgaacca ggtatcgctg gtttcgttgc cggtaaacag
    37081 ggtaatgaag tttttcttcg gcattttgca ctgctcaacg cagagcttca tgatcagacg
    37141 ttcttgcgta cgaacgcggt ccatcatgac gcgcatgctg ttgaccaggt agtcaaactg
    37201 cttcggcacc aggcggaact gtttgaatac ttcagacagt ttcaggatct cttcctgagc
    37261 ggtagcgtga ctgcgacctt tcgctttgat ggtgtcacgc gttacaacgt actgagcgcg
    37321 tagttccgca aatttttcgc gagccagttc cgggtcgatg ctgttgtcat catcggcgct
    37381 gtcgtcatcg ccatcttctt cgtcttcatc ttcgtcatcg tccagatctt cctgggaaag
    37441 ctcagaaccg acgtgagtgg cggtaggtgc cagatcttct tctgcgttcg ggtcaacaaa
    37501 gccggtgatc agatcggaca gacgcgcttc ttctgcttca acacgatcgt actgttccag
    37561 cagataggtg atcgcttccg gatattcagc aacggagcat tgaacctggt tgatcccgtc
    37621 ttcaatacgc ttagcgatgt caatttcgcc ttcgcgggtc aacagttcaa cggtgcccat
    37681 ttcacgcatg tacatgcgta ccgggtcagt cgtgcgcccg atttcagatt ccacgctgga
    37741 aagcacctgc gcggcggctt cggcagcatc ttcgtccgcg gtgttttcag ccagcatcag
    37801 atcatcggca tccggtgctt cttccatcac ctgaatgccc atgtcgttga tcatttggat
    37861 gatgtcttcg atctgatctg aatcgacgat atcttccggc agatggtcat tgacctcggc
    37921 ataggtcaga tagccttgct ccttaccacg ggtgacaaga agtttcagct gtgactgcgg
    37981 gttttgctcc ataagacggt atccacactt atttcatgag gttggtgttg ggcgattgac
    38041 ccgatgtcct tcagccgtta acgctgaaat ccaacggttg tttaccgcca acgataatta
    38101 cgagggcgta cttatatatt tgccgctgcc tctcagtgcg gctgtcgggg gcttcccgat
    38161 cgctcttcgg cacttaagcc gttaaatcac tttttcgcca gctcctggtt taatgtccag
    38221 agctccaggc gttcttcgtt gcttaaacca tgcgtgcgct cacgagcgat taactcttcc
    38281 tggcgcagtt caagcagcga atcaaacata tggttgagtg agtcggtgaa ggtttgctca
    38341 gcaatattct tatctgctat atcgtcccac atcgacagtt tttcaagggt ggcagcatta
    38401 tttgtaccac gatagtgctc taaaagttgc ccggtggtca gacctggctg ggagagacaa
    38461 gtgttgacca gttctctgaa taagccaagt ccagggagct tattttcatc cagattctca
    38521 agcggcggga ccaacgtcgc taattctgga ttttgcacca gcaaccctat aagtatacgc
    38581 atggtcgtgc gttttagctg cggaacaggg cgagaaacgc cgctctctgc cgcttttggc
    38641 attaatcgtt caagctggct gtcatcaagt atgcctaatt tgttgcctaa ttcctgacga
    38701 agatatattc gcagcgtttc gcccggcact tgcgatatca atggtagtgc cagcgtactc
    38761 aaacgtgcgc gcccgtcagg ggtactcaga tcaacttgcg gcatcagact gttaaacaga
    38821 aatgcggaga gtggcatcgc ctgctccatc cgcgcttcaa acgcttcttt accttctttt
    38881 cgtaccagcg tgtcagggtc ttcgccatca ggcaaaaaca taaagcgtag ctgacggccg
    38941 tctgtcatgt aaggcagcgc cgtttccagc gctcgccagg cggcatcgcg gcctgcacgg
    39001 tcgccgtcat aacagcaaat gacattgttg gtcgcgcgga acaacagttg tatgtgatcg
    39061 gcggtggttg acgtacctaa cgacgcaacg gcgtaattaa tgccgtattg cgccagcgcc
    39121 accacgtcca tatagccttc gaccacaagc agacgattgg gttcagcgtt atcctgctgc
    39181 gcttcataaa gaccgtaaag ctggcggcct ttatggaaaa tgtctgtttc cggcgagttc
    39241 aggtatttgg gggtatcgtt gcccagcacg cgcccgccaa aaccaatcac ccgaccgcgt
    39301 ttatcgcgaa tggggaacat cacccgctcg cggaaacgat cgtaactgcg tccctgatcg
    39361 ttagtgacca acatccccgc atcaatcaat gactggcgat tttctggatt gccgccaaac
    39421 cgcttcagga cgttgtccca gccggggggc gcaaaaccaa tcgcaaagcg agcgataacc
    39481 tcgtggctta atccgcgttt ttccagatac tggcgcgcag acgtggcaac aggttgttgt
    39541 aaagattgtt ggtaaaacgt attcagaccg tccatcaact gataaagcgt ttgcctctga
    39601 tggcgctcga tctggctggg gccgctgcct gcttcaaatg gcacttcaag attgtgcatt
    39661 gctgccagct cttcgaccgt ttcgacgaac tcgagcttgt cgtagttcat caggaagtcg
    39721 atcgcgttgc cgtgcgcgcc acatccaaag cagtggtaaa actgtttctc accgttaacg
    39781 gtgaaggacg gggttttctc gttgtggaat ggacaacacg cgtggaaatt cttgccctgc
    39841 tttttcagct tcacacgggc atcgatcaga tcgacgatgt cagtgcgtgc cagcagatca
    39901 ttaatgaata cgcgtgggat tcgtccagcc ataggccccg atttttagca attcataaac
    39961 gaaaataagc cgcgcattcc tttcggaagc acggccttac aactacaact cggtctgatc
    40021 ggagagcaac gctctcgggg aattagtaca gacgagtgcg gcgtgcgttt tcgcgagcca
    40081 gtttcttcgc gtgacgtttc actgcagaag ctttagcgcg cttacgttcg gtagtcggtt
    40141 tttcatagaa ctcacgacga cgaacttccg ccagaacacc tgctttttcg caggaacgct
    40201 tgaagcgacg cagagctacg tcgaacggct cgttttcacg tactttaatt accggcatgt
    40261 gcctctcacc tttgattaat tcggtttgcc gctggcatca acgccagctt atttcaaaat
    40321 ggtgcggaat tttactgcaa ttgctgctgc tttgtaaagc accgcggcct tttttgaaag
    40381 ggacttttat aagggtgagg agtatacacg aggctttctc caggggcgaa caaagtttta
    40441 catcaacccg cattggtcct acactgcgcg gtaataaagc gaggtaaaac aagtcatgcg
    40501 tgtactgggt attgaaactt cctgcgatga aaccggcatc gccatttacg acgatgaaaa
    40561 aggtttgtta gccaaccaat tgtatagtca ggtgaaattg cacgctgact acggcggcgt
    40621 cgtgcctgaa ctggcctccc gcgatcatgt gcgtaaaacc gtaccgttga tccaggcggc
    40681 gctaaaggag tctggtttaa cggcaaaaga cattgatgct gtggcctata ccgcaggccc
    40741 tggattagtc ggcgcgctac tggttggcgc gaccgtgggg cgttctctgg cgtttgcctg
    40801 ggacgttccg gcgatccctg tacaccatat ggaagggcat ctgttagcgc cgatgctgga
    40861 agataacccg ccggaatttc cgtttgttgc gctgcttgtt tccggcggtc atacgcagtt
    40921 aatcagcgtg actggcattg gtcagtacga gctgctcggc gagtctatcg atgatgccgc
    40981 cggggaagcg tttgataaaa ccgcgaagct gctggggctg gattatcctg gcgggccgtt
    41041 actgtcgaaa atggcggctc agggtactgc cgggcgcttt gtcttcccgc gtccgatgac
    41101 cgaccgtccg gggctggatt tcagcttctc cggcctgaaa accttcgcgg caaataccat
    41161 tcgtgacaac ggcaccgacg accagacgcg tgctgatatc gcccgcgcct ttgaagatgc
    41221 ggtggtcgat acgctgatga ttaagtgcaa gcgggcgctg gatcagacgg gctttaagcg
    41281 actggtcatg gcgggcggcg tgagtgctaa ccgtacgtta cgggcgaagc tggctgaaat
    41341 gatgaaaaaa cgccgcggcg aagtgttcta cgcgcgtccg gaattttgta ctgataacgg
    41401 cgcgatgatc gcctatgccg gaatggtgcg gtttaaagca ggcgcgacgg cggatctcgg
    41461 cgttagcgtg cgtccgcgct ggccgctggc ggagttaccg gctgcgtaaa actattgggt
    41521 gccggagagc aatttccggc accgtcctca cttaaagcaa caccacgggc atccacagta
    41581 agccggtaat caccagcaat acgaggaata tcagcccaaa aatcgccccc agtcgccagt
    41641 aatccgccgt tggcagataa ccactgccgt agtaaatcgg actggggccg gtggcgtatg
    41701 gtgtaagaat gctccccaga ccaattgccg caccaaccat caggcagaac accggcagcg
    41761 ggatttccgg catcgccagc gcagcggcaa tcatcatcgg tgcgagagcg gaggtatacg
    41821 ccgtggcgct ggcgaaaaag tagcgcagta gataaaacac cacaatcaac gccaccatca
    41881 ccatcgttgg cgaataaccg cttaagctgc ctgctaacag tttgccaaac cagctaataa
    41941 aaccggtgtt gttgagtccg gtggcgaggg tgataagcga ggccagccag aagaaaacgt
    42001 tccacgccgc tttattactg acaatgtcgt cccaactgat aatgcgcaga agcagcatca
    42061 gtgccaccac gctgtaaccg accatcgcgg catcgatata atcaccgccg aaaatccaca
    42121 gcaccagcgc gcctaccatc agccccagca tccgtttttc acgcgaacag agcgggccca
    42181 ttgcctgcag ttccgtctct gcccagcgcg gcacctgatc gccagacttc agtaccggcg
    42241 ggtacagcac gtaagccagc cagggaacca gcagaaccag taaaatgctg agcggcaaca
    42301 tcccgaggaa ccagtcgccc caactcagcg tggcgtgaga tgcgcttttc atcagtccaa
    42361 ttaacagcaa gtttggtgcc atcgccgtca agaaaatggc gctggtcacg cagtcggcaa
    42421 caatccccat ccacatgatg tacgagccaa tgctgcgcga actgctgtcg tttggttgtg
    42481 attgatagag cggtggcagg ttacggatga tgggatagat aatccccgca ccacgcgccg
    42541 agttggacgg tgttacaggt gcgaggatca gctcggagaa catcaccgca tagccgagaa
    42601 acagcgtgcg atgtcccatc tttttcacca gaatcagcgc aatgcggcgt ccaagcccgg
    42661 ttttttcata gcctgtgcca aacataaagg cggcgaaaat cagccagata accgaattag
    42721 aaaaaccgga aacggcccac gagagggatt ttgcagtgaa tttaaagcct ggctgagcga
    42781 gctgctccgg gctgaacagc agccagggag agagtatggc gatgatggat atacccacca
    42841 tcgccaccac ggcacccggc acgggttcga ggatcagtcc aacgatcacg ccagtaaaaa
    42901 cggcaaagta gagccaggta tggttctcca gccctgcggg aaccggaagt agagcaataa
    42961 tggcgatgac cgccagcggc gcaagatatc gccaccattc agtggaaggt ttcatcgtgt
    43021 tatgcctgcg aaaaaggaga ggggtgagcc tctccgggag ggttatttga tgtaatggac
    43081 gtgctcgcag atctcttcca cgatgggatc gcggcgttcg gcgaacagct ttttgttttc
    43141 ggctatcagg ttgttgccgt gggtatcaat agagacaatc agcgggccga actctttgac
    43201 ccggcagacc cacagtgact ccggcattcc gagctctgtc cagtgcactt cttcaatctc
    43261 ttccacctgg gttgccgcca gcaccgcgca gcctgccggg aaaatcacat gtagcgcctt
    43321 gaatttctgg cagccttctt cggtcagcgg ccccatacca cctttgccaa ccaccagttt
    43381 cacgccggtc tgctcaataa attcacgttc aaaactttcc atacgcatac tggttgtcgg
    43441 gccgacggag accatctccc atttgtcgcc gtttttgcgc acgatggggc cagcgtggaa
    43501 aatcgctttg ccgttgagat cgtaagggat cggacgtttc agttcgatca aacggcggtg
    43561 acaaacgtcg cggcaggtca ccagcgtacc ggtcaggtag atcacatcgc cgacgcgaat
    43621 atcttgcaga tcttcagctt tgatcggggt tgtcaggatc tttttcataa cgcgctccgg
    43681 gtgtgagaca gattttcaaa ggtgagatcg gcatgaacca gcagcgtgcc gcgacgatgc
    43741 gcccagcagc cggtagagac agcaacgccg atggttgacg gatggcgggc ggcagattcg
    43801 atatgtacgc ccatcactga actgttgccg gtcagccctt gtggaccaat ccccagacgg
    43861 ttgagtcctt cttccaggcg tagctccagt tctgccgctt ttggattggg atggcggctg
    43921 ccaatcgggc gcaaaatggc tttacgcgag agtacggcgg cggtttccac cgaggtggcg
    43981 atgcccacgc ccaccagtac cggtggacag gcgtttacgg cgagggtgga gatattttcg
    44041 aagacgaatt tcaccacgcc ttcgtagcct tctgacggca ttaacacttt cgagcggcca
    44101 ggtagcgtgc agccgccgcc tgccatgtaa acttcgattt ccgcatcgtc attgtcgggg
    44161 atgatgtccc aggtgaccca cggtacgccg ctaccggtat ttttgccggt gtttacttcg
    44221 tcaaaaattt ctaccgcatt gtgacgtagt ggcgctttga cggttgcctc ttccacggct
    44281 tgtttgagta tgctttgcag ctcgccaagc agtgggaagc gggaaccgac tttaacgaag
    44341 aacataatct ccccggtgtc ctgacaggca ggacgattca ggtcaatcgc tttttgcatg
    44401 ttgtcgaaca tcgtatggta gataattttc cccatcgacg acgtttcggc atcctttagc
    44461 tgttttagtt tatccaccac gtcatcaggc attcgggtag aaatcatggc ggtaaagtta
    44521 gcgacaatct ctgtcaactt attcactgcc tgttgcttat tactttcgct catcatggca
    44581 actccagttt ttgtatttgt ctggaaaaat tcaccgccac aaactaccac taaaaatgtg
    44641 gttttatatt ttttgccacc gtgaatatgt aattaacagt tcgtgaatca acgcgaaaat
    44701 aagaatgaat attgcgccag agcagggaat ggcaatgttt tgcgaaatac actgcgctta
    44761 tcacaactgc ggataattaa cgatgctgaa tagctggcct ttagccaaag acttgcaggt
    44821 actggtagaa atcgttcaca gcgggagttt tagcgccgca gcggcgacgc ttgggcagac
    44881 gcctgcattt gtcaccaagc ggatccaaat ccttgaaaat acgctggcaa caacgctgct
    44941 gaaccgctcc gctcgcggcg tggcgctgac tgaaagtggc caacgttgtt acgaacatgc
    45001 gttggaaatc ctcacccaat atcagcgact ggtcgatgac gtcacgcaaa tcaaaacgcg
    45061 cccggagggg atgattcgta ttggttgcag ttttggtttt gggcgcagcc atattgcgcc
    45121 agctattacc gaactgatgc gcaattatcc tgagttacag gtgcattttg aattgttcga
    45181 tcggcaaatt gatttagtgc aggataatat tgatctggat attcgtatta atgacgaaat
    45241 tcctgattat tatattgcgc atcttttgac aaaaaataaa agaatattat gcgcagcacc
    45301 tgagtatctg caaaaatatc cgcagccaca atccctacag gaattaagtc gtcatgactg
    45361 tctggtgacc aaagagcgcg atatgaccca tggaatatgg gagttgggga acggtcagga
    45421 gaaaaaatcg gtgaaggtaa gcgggcatct ttcctccaat agcggcgaga ttgtgctgca
    45481 atgggcgctg gagggcaaag ggattatgct gcgttccgag tgggatgtgt tgccgtttct
    45541 ggagagtggc aaactggtgc aagtattgcc agagtatgca caaagcgcca atatctgggc
    45601 tgtttaccgg gagccgctct atcgcagcat gaaattacgt gtctgcgtgg aatttctggc
    45661 ggcatggtgc cagcaacggc tgggcaagcc cgatgaaggc tatcaggtca tgtagatcca
    45721 ccagaaatca ctcgggatcc ttttcgcgct ttcttttgaa tttcgtccag atttttgtct
    45781 cctgacgacg ccacagacgt tggatgttgt catgatgacg cagcaggatc aggcaagaga
    45841 gcatcgaaac cgggaaggtg aattgtggct taaaccacca gacataaaac ggagcaatca
    45901 gtgcactgac aatcgctccc agcgacgagt atccgctcaa tagcacggtc agtaaccagg
    45961 ttcccgccat tactccggtg agatcccagc caatgggtgc gatggcacca aaagcggtag
    46021 caacgccttt tcctccttta aatccgaaga aaacgggcca gatgtgtcca agacaggcgg
    46081 cgatggcaat taagcctagc cagaaggggc tgacacctaa ttcatacgcg ccccagacgg
    46141 gcaacattcc tttcagaacg tcgaaaatca gtactgctac ggctgctccc ttgccaccga
    46201 tacgtaacac attggttgcg cctggattgc cggagccgct ggttcgcgga tcgggcagcc
    46261 cacacaagcg gcaaaccaga atggcactgg aaatggagcc gcagaggtac gcgatgagga
    46321 tcattccagg cgcgattgca ctcataagct gttccgtttt gaaaattcgt gttcaacgat
    46381 gaatctgtgg ataatacgca catttcgccg gaagtggtat ccggttagcc aaaaagcagg
    46441 caggacgtga tggatattgt atttatagag caactttcgg taatcaccac tattggtgtt
    46501 tacgactggg aacagaccat cgaacagaag ttagtgttcg atatcgaaat ggcgtgggat
    46561 aaccgtaaag cggcgaaaag tgatgatgtg gcggattgcc tcagttacgc tgacattgca
    46621 gaaacggtgg tcagccacgt cgagggggcg cgttttgcgc tggtggaacg cgtggctgaa
    46681 gaggtggcgg agctgctgtt agcacgcttc aactcgccgt gggtgcgtat caaactcagc
    46741 aagccaggcg cagtggcgcg ggcggcgaat gttggcgtaa tcattgagcg tggcaataat
    46801 ctgaaagaaa ataattaatt ttacagctgt taaaccaaac ggttataacc tggtcatacg
    46861 cagtagttcg gacaagcggt acattttaat aatttagggg tttattgatg agcgatatgc
    46921 actcgctgct gatagcggca atattgggtg tggtcgaagg attgacagaa tttctgccgg
    46981 tatccagcac gggccatatg attattgtcg gtcacttgtt ggggtttgag ggcgacacgg
    47041 cgaaaacctt tgaagttgtg atccagttag gatcaattct ggcggtagta gtgatgttct
    47101 ggcggcgtct gtttggcctg attggcatcc actttggccg cccgttgcag cacgaaggtg
    47161 aaagcaaagg tcgtttaacg ctgatccaca ttttgctggg gatgattccg gcggtggtat
    47221 tggggctgtt gttccacgac acgattaagt cattgtttaa cccgataaat gtgatgtatg
    47281 cgctggtcgt tggcggtttg ttgctgattg ccgccgaatg cctgaagccg aaagagccgc
    47341 gtgcgccggg tcttgatgat atgacctatc gtcaggcatt tatgattggc tgtttccagt
    47401 gtctggcgct gtggccgggt ttctcccgtt ccggggcgac catttcaggt gggatgctga
    47461 tgggggtgag ccgttacgct gcttccgagt tttcgttcct gctggcggtg ccgatgatga
    47521 tgggcgcaac ggcgctcgat ctctacaaaa gctggggctt cctgacaagc ggcgatatcc
    47581 cgatgtttgc cgttgggttt atcaccgctt ttgtggtggc gctgatagcg attaaaacct
    47641 tcctgcaatt gattaagcgc atttcgttta tcccgttcgc catttatcgc tttattgtgg
    47701 cggctgcggt gtatgtcgtg ttcttttaat tgaggaatag tgtcggatgc ttggcgttgt
    47761 caacgattgc gtaggccgaa taaggcgttc acgccgcatc cggcaatcaa cgcctgatgc
    47821 gacgcttgcg cgtcttatca ggcctacaac tgtcgttgac atgtagccgg ataaggcgtt
    47881 tacgccgcat ccggcaaaat gcccaatact cattcaggct ttgggcaacg ttgttccttc
    47941 cagctggcta ccgccgcaat ccgccgtcgg gtcagctcct cgcgaatctc cacaccttta
    48001 aatcccgctt caacgacggc ttttgtcggc actgactgcg ccacttccca ggcttcgcgc
    48061 aaccagcggc cttgcgggta gtccgctgat tcaaaaccgg ttctgccgcg cacgtcagcc
    48121 tcgctggtca gcgccagttg ctcgacacgc tgcggtttac gccaggcatc gatggaatca
    48181 aataatttga cgatggtttt tgggttcagc attgggaagg tgtggatgag atcgtgaaac
    48241 tcagccacca gtctggctaa atcgcgaatt tcatttggca cgcgcagacg ctggcatagt
    48301 tgttccacta acttaacacc cgccgggcca tgaccatgat gacgcggcca gagttctggc
    48361 ggcgtcagcc ctttaccgag atcgtggcat aaagttgcga aacggacatc gacctgcgga
    48421 ctcagcatcg ccgccattga gagcgtcatt aaggtatgaa tacccgtatc gatttccgga
    48481 tgccacttgg caggggccgg aacgccaaac agtgcgtcaa tttccgggaa taaaacgcgc
    48541 agtgcgccgc aatcgcgcag tacctggaag aacacctgtg gattgcgggt ggtgagggcg
    48601 ctttccgtct ctttccatac ccgttcaggc gtcaggtgtt ccagttcacc cgcatgggtc
    48661 atctcgcgca tcaacgccag agtttcatcg gcaatacgaa aaccgaggtg ggcataacgc
    48721 gcagcaaaac gcgccacgcg caatacgcgt aacggatctt cgccaaaagc gggggaaaca
    48781 tggcgcaaca gacgattttg cagatcgccc agaccgttgt acgggtcgat aatctcgccg
    48841 ttatcgtcct gggccagcgc attaatggtc agatcgcgac gcttaagatc atcttccagc
    48901 gtgacatccg gtgcggcata gcaagtaaaa ccggtgtaac cggaaccgga tttccgttcg
    48961 gtacgtgcca gcgcatactc ttcatgcgtt tgcggatgca gaaacacagg aaaatcgcgg
    49021 cctacctgct ggtagcccgc gtcgagcatc ttctgtggcg tactgccgac caccacccaa
    49081 tctctgtctt tgaccggtag ccctaacaat gcatcccgaa cagcaccacc gaccagataa
    49141 atcttcacgc catcatccct tcgtcaaata cacaattcct aataataaga cagtgcaacg
    49201 aagaaggcga tttagttcat ccagcgatct ttgcgtttgc ggcttgggat caggtgcggc
    49261 agtaccagac cgagcagcaa gccaagcccc agcacgccac cgccatacat aaaccattgc
    49321 atgatgatgg tgcgctgttt gtcatccagc tgtacgctgg cggcatcgac ctttttctgc
    49381 gcgacaatca gctcgttttt cagtttctga ttttcttctt ttaacccgtt gatcacgctg
    49441 tcgctctgcg ccactttttg ctgcatttct gcggtgcgct gattccaggt gttatcgata
    49501 ttggtgagtt tatcggtcag ggttttgacc tgattttcca gatctggcac acgggagcgc
    49561 aggcttggct cagtgctaag ttgtttcaac gggatccagg cggtacggcc agagctgtct
    49621 ttcacctggg cataattggt gttggcgtca gtttgtaata aggtcacttc ctcgccggcg
    49681 ttaaccgtgc ccacgaggcg ataatgatct cccggaccgc tgcggaccca ggtattcagt
    49741 tcgtcggaaa catagcgcgt ttcttcagcg tgtgagacgg cagtcgcgct aagtgcgagt
    49801 aaagttaatc cgatcaggcg taattttggc atcaggctgt cgttattgtc atggaaagtg
    49861 gaacgatagt agtggcatca gtgtcgctac gcaaagcatt cgacatcaat cggaatcctc
    49921 tgtgtcaggt cttgccactg cttcaaactt ttacgcccgt caaattgccc gttgcatggc
    49981 aatttggcgc aaaatactat ctactgacaa aaaagatcgc attaagttcg ccgttcatcg
    50041 tcacttatgt gacataaccg ataagtaaag gccatggctc aggaaatcga attaaagttt
    50101 attgttaatc acagtgccgt tgaggcgttg cgtgaccatc tcaatacgct gggcggcgag
    50161 caccatgacc ccgtgcagtt gctgaatatt tactacgaaa cgccggataa ctggctgcgt
    50221 gggcacgata tgggcttacg tattcgtggc gaaaacggtc gctatgagat gaccatgaaa
    50281 gttgcaggaa gagtgacagg cggcttacat cagcgcccgg aatataacgt ggcgttgagc
    50341 gaaccgacgc tcgacctggc gcagttaccg acggaagtct ggccgaacgg cgaattgccc
    50401 gccgatctcg cctcccgcgt gcagccgctg ttcagcaccg atttttatcg cgaaaaatgg
    50461 ctggtggcgg tcgatggtag ccaaattgaa atcgccctcg accaggggga agtgaaagcg
    50521 ggtgaatttg ctgaacctat ctgtgagctg gaactggaac tgcttagcgg cgacacgcgc
    50581 gcggtgctga aactggcgaa ccaactggta tcgcaaaccg gattacgcca gggcagcctg
    50641 agcaaagcgg cgcgtggcta tcatctggcg cagggcaatc cggcgcgtga aatcaaaccg
    50701 accaccattt tgcatgttgc ggcaaaagcc gatgtggaac aggggctgga agcggcgctc
    50761 gagctggcgt tagcgcaatg gcagtatcat gaagaactgt gggtacgcgg caacgatgcg
    50821 gcgaaagaac aggtgctggc agccattagc ctggtccgtc atacgctgat gctgttcggt
    50881 ggtattgtgc cgcgtaaagc gagcactcac ttacgtgatc tgctgactca atgcgaggcg
    50941 accattgctt ctgcggtgtc tgccgtgacg gcggtctact ctaccgaaac ggcaatggcg
    51001 aagctggcgt tgaccgaatg gttggtaagc aaagcatggc agccattttt agatgccaaa
    51061 gcgcagggca aaatcagcga ctccttcaaa cgctttgccg atatccatct ttcccgccat
    51121 gccgctgaac tgaaaagcgt tttctgccag ccgttaggcg atcgctaccg tgaccagttg
    51181 ccacgcctga cgcgtgatat tgactcaata ctgttgctgg cgggttacta tgatcctgtc
    51241 gtcgcgcaag cctggctgga gaactggcag gggctgcatc acgctattgc gaccgggcaa
    51301 cgcatcgaaa ttgaacattt ccgtaatgag gcaaacaatc aggaaccgtt ctggttgcac
    51361 agcggaaaac gttaatcagg caaggataaa atcgcttatg aagccgctct cttcaccgtt
    51421 acagcagtac tggcagaccg ttgttgagcg gctgccagag cctttagccg aggaatcact
    51481 tagcgcacag gcgaagtcag tacttacttt tagtgatttt gtgcaggaca gcgtgattgc
    51541 gcatccagag tggctgacgg aactggaaag ccaaccgccg caggccgacg aatggcagca
    51601 ttacgcggca tggttgcagg aggcgctctg taatgtgagt gacgaagccg ggttaatgcg
    51661 cgagctgcgg ctattccggc ggcgcattat ggtgcgcatc gcctgggcgc aaacgctggc
    51721 actggttact gaagagagca tattgcagca gctcagctat ctggcggaga cgctgattgt
    51781 tgcggcgcgt gactggctgt atgacgcctg ctgccgcgag tggggaacgc cgtgcaatgc
    51841 gcagggcgaa gcgcaaccgc tgctgatttt aggcatgggt aagctgggcg gtggggagct
    51901 gaatttctcc tctgatatcg atctgatttt tgcctggccg gaacatggtt gtacgcaggg
    51961 tggacgccgg gaactggata acgcgcagtt ttttacccgc atggggcagc ggctgattaa
    52021 agtgctggat caaccaacgc aggatggctt cgtctatcgc gtggatatgc ggctgcgtcc
    52081 gtttggcgaa agtggcccgc tggtgctgag ctttgccgcg ttggaagatt attaccagga
    52141 gcaggggcgc gactgggagc gttacgcgat ggtcaaggcg cggattatgg gcgatagcga
    52201 aggcgtctat gctaacgagt tgcgtgcgat gctgcgcccg tttgttttcc gccgttacat
    52261 cgatttcagc gtgattcagt cgctgcgcaa catgaaaggg atgattgccc gtgaagtgcg
    52321 tcgacgtggt ttgaccgaca atatcaaact tggcgcaggc ggcattcgcg aaattgaatt
    52381 tatcgttcag gtgttccagc tcattcgcgg tgggcgcgaa ccgtcgctgc agtcgcgctc
    52441 tttactgcca acgctcagcg tcattgctgc gctgcatctg ctttctgaaa acgatgctga
    52501 acaattgcga gtggcgtatc tgttcctgcg gcgtctggaa aacctgctgc aaagcattaa
    52561 cgacgaacaa acccagacgc ttccttctga tgagcttaat cgtgcgcggc tggcgtgggc
    52621 gatggacttt gctgactggc cgcaactgac cggggcgctg accgcacata tgaccaatgt
    52681 gcgccgggtg tttaatgaat tgattggcga cgatgaaagt gaaactcagg aagagtcgct
    52741 gtcggaacag tggcgtgagc tgtggcagga tgcgttgcag gaagatgaca ctacgccagt
    52801 gctggcgcat cttagcgagg atgatcgcaa acaggtgcta acgctgattg ccgatttccg
    52861 caaagagctg gataagcgca ccatcgggcc gcgaggacgt caggtgctcg accatctgat
    52921 gccgcatctg ctaagtgatg tctgtgcgcg tgaagacgct gccgttacgc tgtcgcgcat
    52981 taccgccttg ctggtgggga ttgttacccg caccacctat ttagaattgc tcagtgaatt
    53041 ccccgcggcg cttaaacatt tgatttctct gtgtgccgcg tcgccgatga ttgccagcca
    53101 gctggcgcgt tatccattat tgctggatga attgctcgat ccaaacaccc tttaccagcc
    53161 gacggcgacc gatgcctacc gcgatgagtt gcgccagtat ttgctgcgcg tgccggaaga
    53221 tgacgaagag caacagcttg aggcgctgcg tcagttcaaa caggcgcagc tgttacgcat
    53281 cgccgcagcg gatatcgccg gtacgctacc ggtgatgaaa gtgagcgatc acttaacctg
    53341 gctggcggaa gccatgatag atgccgtcgt tcagcaggcg tgggttcaaa tggttgcccg
    53401 ctacggtaag ccgaatcacc tgaacgaacg cgaagggcgt ggttttgcgg tggtcggcta
    53461 cggcaagctg ggcggctggg agttaggcta cagttccgat cttgacctta tcttcctcca
    53521 tgattgccca atggatgcga tgactgacgg tgagcgggaa atcgacgggc ggcagtttta
    53581 tctgcgtctg gcgcaacgca ttatgcatct gttcagtacg cgtacctctt ccggcatttt
    53641 gtatgaagtg gatgctcgac tgcgtccgtc cggggcggcg ggaatgctgg tgacatccgc
    53701 agaagcattt gccgattatc agaaaaacga ggcctggacg tgggaacatc aggcgctggt
    53761 gcgtgcgcgt gtagtgtacg gcgatccgca gctcaccgcg cactttgacg cagtgcgtcg
    53821 cgagattatg acgctgccgc gtgaaggtaa aactctgcaa acggaagtgc gggaaatgcg
    53881 cgagaaaatg cgcgctcatc tcggcaataa acatcgcgat cgctttgata tcaaagctga
    53941 tgaaggggga attaccgata tcgaatttat tacccaatat ctggtgttgc gctacgctca
    54001 tgaaaaaccg aagttaacgc gctggtcaga caacgtgcgt attctggaac tactggcgca
    54061 aaacgacatt atggaagagc aggaagcgat ggcgctgacc cgtgcttaca ctacgcttcg
    54121 cgatgaactt catcatctgg cattacagga attgccgggc catgtgtcgg aggattgctt
    54181 caccgcagag cgtgaactgg tgcgggcaag ctggcagaag tggctggtgg aagaatgaag
    54241 tatggtatta tcgcgcgcaa attttgaatc tctcaggaga caggaatgaa agtaacgctg
    54301 ccagagtttg aacgtgcagg agtgatggtg gttggtgatg tgatgctgga tcgttactgg
    54361 tacggcccca ccagtcgtat ctcgccggaa gcgccggtgc ccgtggttaa agtgaatacc
    54421 atcgaagaac gtccgggcgg cgcggctaac gtggcgatga atatcgcttc tctcggtgct
    54481 aatgcacgcc tggtcgggtt gacgggcatt gacgatgcag cgcgcgcgct gagtaaatct
    54541 ctggccgacg tcaacgtcaa atgcgacttc gtttctgtac cgacgcatcc gaccattacc
    54601 aaattacggg tactttcccg caaccaacag ctgatccgtc tggattttga agaaggtttc
    54661 gaaggtgttg atccgcagcc gctgcacgag cggattaatc aggcgctgag ttcgattggc
    54721 gcgctggtgc tttctgacta cgccaaaggt gcgctggcaa gcgtacagca gatgatccaa
    54781 ctggcgcgta aagcgggtgt tccggtgctg attgatccaa aaggtaccga ttttgagcgc
    54841 taccgcggcg ctacgctgtt aacgccgaat ctctcggaat ttgaagctgt tgtcggtaaa
    54901 tgtaagaccg aagaagagat tgttgagcgc ggcatgaaac tgattgccga ttacgaactc
    54961 tcggctctgt tagtgacccg ttccgaacag ggtatgtcgc tgctgcaacc gggtaaagcg
    55021 ccgctgcata tgccaaccca ggcgcaggaa gtgtatgacg ttaccggtgc gggcgacacg
    55081 gtgattggcg tcctggcggc aacgctggca gcgggtaatt cgctggaaga agcctgcttc
    55141 tttgccaatg cggcggctgg cgtggtggtc ggcaaactgg gaacctccac ggtttcgccg
    55201 atcgagctgg aaaatgctgt acgtggacgt gcagatacag gctttggcgt gatgaccgaa
    55261 gaggaactga agctggccgt agcggcagcg cgtaaacgtg gtgaaaaagt ggtgatgacc
    55321 aacggtgtct ttgacatcct gcacgccggg cacgtctctt atctggcaaa tgcccgtaaa
    55381 ctgggtgacc gtttgattgt cgctgtgaac agcgatgcct ccaccaaacg gctgaaaggg
    55441 gattcccgcc ccgttaaccc gctcgaacag cgtatgattg tgctgggcgc actggaagcg
    55501 gtcgactggg tggtgtcgtt tgaagaggac acgccgcagc gcttgatcgc cgggatcttg
    55561 ccagatctgc tggtgaaagg cggcgactat aaaccagaag agattgccgg gagtaaagaa
    55621 gtctgggcca acggtggcga agtgttggtg ctcaactttg aagacggttg ctcgacgacc
    55681 aacatcatca agaagatcca acaggataaa aaaggctaac cggaaagcgg ttcacagatc
    55741 tcgcttcgta catggcaggc ctattgacgc tgttgttttt tcaacctaga gtaaaggaac
    55801 aagggtaagg gaggatttct cccccctctg atgagttgtt agtaagtcgg gaaacttaac
    55861 agtaacaaca caaccagtat gatgacgaac ttcatcataa ccctttcctt atacaaggcc
    55921 ccttcttcgg gaggggcttt cccgtttcag ccccttgctg ccccccgatt gttgatgagc
    55981 gccggacccc gcgcaatgct ttcccagagg caattttgcc ttccccgagc ggtcacgcaa
    56041 aacgctgcaa cgtcctgagc atttgcacct tttagcggaa cgcagatata aaaaatcccc
    56101 ggctttttgg gccagggata tgtgttaatg aaaccttctt gacgctgatt ttttttcaac
    56161 ctaaagtaaa ggaacaaggg tgagggagga tttctccccc ctctgattgg ctgttaataa
    56221 gctgcgaaac ttacgagtaa caacacaatc agtatgatga cgagcttcat cataaccctt
    56281 tccttctgta aggccccttc ttcgggaggg gctttcccgt ttcagccccc tgctgactcc
    56341 ccgattgttg atgagcgcca gaccccacgc aatgctttct ctgatgcagt tttgccttcc
    56401 ccgagccgtc acgcaaaatg ctgcaacaac ctgatcattt gcacttttta gcggaacgca
    56461 gatataaaaa atccccggct ttttgtgcca gggacgttca tagctgaaaa tttattctac
    56521 gttttcggcg acgggagttg ttgaggttaa gggtgaagtc aatgccgtca gtgagccgcc
    56581 tgaaacacca atttcattga gcaaggagtc aatcagcggt gcctgtgtgc ggtaagaaag
    56641 ggcggcagac aatgcctgct ctgcaaggtt tcctccacta acgctacctg tactcgcatc
    56701 acccgcagcg ccgccacgat tcagaccatc gacctgaata atcttgatac cgtcgattga
    56761 cttcatcggc tcaacggatt tctctattac cgtaggcagc gcctgcaaca aggccagttt
    56821 gaatttaagg ctggtttgtt catcagaaag tacgttgata gcatcgttca gcgcacgttg
    56881 tgcttctgct tccgccagac cctttttacg tgtagcttcg gctaactcaa cgatagccgc
    56941 cgcctgcatc tctgcggctt ctttttctgc tttcgcccgc acggtcagtt caaccgcttt
    57001 ggtttctgca tcctgcgcgg cagcgattag ggcaacttgt ttagcacgat cggcttcggc
    57061 agtctggcga gtggtttcta cgttttgttg ggcgcttacc gcttctgcaa gtgcgagatt
    57121 agcacgcgct tctgcctggg actgttgttc cgatttggcg gcaatagcga tcgatttcgt
    57181 ctggttagcg atttcggtga cctgctgctg ttcgatctct ttaatgcgaa cttcacgttc
    57241 agcttcaacc tttcttgagc ggacggcctg ttcgcggtcg atttctgttt cctgaatctg
    57301 tcgttcagcc agaattcgtg tctgctctgc ttcacgacga cgttcagctt caaaagccgc
    57361 aatacgtgca ttctgttcgg cagtacgggt tttaacctgc tgctcctgct caagcgtcat
    57421 aaacgcttct tgctgttcaa tctccagctt gcgcgatagc gcatcgcgat ttttttcacg
    57481 caccgcaact tctacatcct gttcaacttc gttacgttcg cggcgacggc gctctgtttc
    57541 ctgagtcagt ttggttaaac cttcggcgtc aaaggcattg ttcggattga aatgttcttt
    57601 cgaggtctgg ttaaagttgg taagtgaaat gctctccagt tccagaccgt ttttcgacag
    57661 gtcttctgcc actgtatttt gcaccccctg cacgaagttc tcgcgggtat cctgtaactc
    57721 atgcatggtc atttgcgcag ctgttgcacg gagggcatcg acaaatttat cttcaaccaa
    57781 catacgtaag tcttcaggcg acagggtgcg ttgccccagc gtctgggcag cggtggcaat
    57841 cccttctact gaaggtttta cccgcacaaa gaaagcgact actacatcga cgcgcatacg
    57901 atctttcgta atcaggctat caatggttga gcggctgact tccagcttca gagtattcat
    57961 attgatgggg attatttcat gaaagatcgg catcacgatt gcgccaccgc tcattaccac
    58021 tttttgccca cctaaaccag tacgaacaaa agcttgctct gccgaagcgc gacgatagag
    58081 cctggtgaaa ataattccaa taataaacag aatgcatacg gcaataatcg cggtaaacat
    58141 ccaggagggc acagaattaa caatatcatc cattattaaa tttccttttg attaataaat
    58201 tacaatattt ggggccaggg attgttttcc gccaaatatc gcgttgcaga aagtcggcaa
    58261 ataattaaca ctttgtctcc tttagtgaaa atttttcctt cttcaggctc cagtagtaaa
    58321 taatgtattt ggccaaattg atcggtgagt tttccttcgc agggattacc tgaagtggct
    58381 tgatgaccgg taattaacgc catgctacca atatattctt cttctgtaat agctgaacta
    58441 tggtcgcgtg gaatccaggg cgcaactatt ttaccggtat aatgcaccgc aataatcgta
    58501 aacagtaaac tgacaggaac cacgaacagg ttcgacagtg gtgattgcca gaccatgatg
    58561 caggcatgct gcaacaggat gccaataaga ccaaaaaaac cagccagtaa gcagagaacg
    58621 acgagagctg gcaatcttcc aatattgaga taaagaagtg cctggctaat atgacctgtg
    58681 gttatggaat cataatggtc aagatgtgca tcaagagcac cggagagcat gtgaccgcag
    58741 ataagcgcaa aaatttctaa caggccgatc aacagtacaa aggaaatagc gaaaagataa
    58801 ggggtgttat agtcggcgaa taaaatcata tatcactcca atgtccattt agtgacgcaa
    58861 tatttacttt gtaatagttt ccttgcttac atttatctgt gttaataata acacaggtta
    58921 gagaagttct ggaagagaaa gtcttttaat atggtttttt atttttcact aaaaagtgtg
    58981 atcggggaca atatatttac gcacgttatg tttaaaggca ctacactgat tggggaatac
    59041 tgaaatcaga aaaagcatat ggccttaaaa ggtatgcgat aaccggagga tgtgcttatg
    59101 gatcatagtc ttaattcttt aaataatttc gatttcctgg cgcgtagttt tgccagaatg
    59161 cacgcagaag gtcgcccggt cgatattctg gccgttactg gtaacatgga tgaagaacat
    59221 agaacctggt tttgcgcacg ttatgcctgg tattgtcaac agatgatgca ggcaagagag
    59281 ctggagttag agcactgata taacgggcct gatggcccgt tttagtgttt gtattagata
    59341 ctagttatag gtgattttca ggattacgtc tttactaaac ggtccctcaa caaccgtttc
    59401 tgccgggttc gcactcagtt ctgccctgaa tggtgcggtg accgaaggcg cactagacga
    59461 tgaaaaagaa gcaaattgtg agaagcggtt aaattccgtt gctgttccat cgttatgaaa
    59521 tatcttcaat agcagaccat tacctatatc cagatggcta taatcactca aaatatccgt
    59581 agtgaaaaaa ctccctaaaa tattaaactg ctcagtacag gccgcacccg gatctttggt
    59641 catgactacg ctaaacgtct cactcacgct ggtattttta attgttcggg aatttatttt
    59701 accaaagtcg acaacgccat cagctggttc cagatcaaca ttaacaaagc agtcaaggac
    59761 atgaatatta tttaacccac gaattctgaa ggtcaaattt ttgtctgtgt ctatagttcc
    59821 tgtgccactc atacctttaa actccagggc gatatattcg tcaggtaata caagtttttt
    59881 atttacttta ttagcatttt tagccagctt aatatacagc tgtacacgta ctggaaaagt
    59941 tactgacaat tgaggttcag gaccaccgct acagacagcg gggtttttat aggtattact
    60001 gtatatccct gcgcctttaa catcaatgca ctctggtgct gcgatagctc gtgtggaggt
    60061 tgaatcataa tcgactccat tataggttac accaagagta tatgagtttt gattataaaa
    60121 gtcctgacct gatgctaaca acatcattat atttacccat gcagatgggt attcttctcc
    60181 tggtgatggg gcgggtagtg cattattaca gaagacattc ataaaatatt cactgctttg
    60241 ccagatttta tcaccttttt taccatcgct ggggatagca attcttggta cgtagcctat
    60301 gactacggta ttcccccctt catgttcagc atagcagttc atcgcccata caggtaacgc
    60361 actaaaaccc ataataaatg caattacagt taataagtag cgcattttat ttatctcgtt
    60421 tggctacgga aacattacaa gaattattca ctgttgggca gtttatttca taaaaactta
    60481 agccaccaaa atcattgata taaccaacct gaaaatttga tggaacattt ttaaactgcg
    60541 tatctgcatg gctaaaaggg aggaccatta agctttgcgt ttgcggtaac atcgtgaccc
    60601 catttgttcc gatataaccc agcgtaacgt gataaggcat tttattgtta aagcggatcg
    60661 aaccatcatt tctacggatg atttcaactt tttcgtaagt cattacccca tcgtcttcta
    60721 actcaatggc ttttggccgc cagaataatt tcaaagcgtg ctgcatagtc acttgcatca
    60781 catttttttt atctgttttt ggcggtattt ctcgcacgtt aaaatagaac agagtttctc
    60841 tgtccttagg aagttgagca attttttcct gagttatcat tctaatctga atttgctcat
    60901 tgggatttaa acgcaccata ggaggaaccg taacaataaa gtcacgagat ttattgcctt
    60961 tcgtatcttc tatccatgtt tgggccagat agggcagttt tttactttga ttagtaagtt
    61021 ttaatgcttc gcctttatct gactcattca ttattatacg ggtacgatcc gggatgatgg
    61081 caccccaggc aatgctgttc atgcaggaga gtaatactcc tgctgcaatg atattctttg
    61141 tatttaagta gcgcattagt ttcatcctga attaatttta gctaatacac ggtaaaataa
    61201 ccgttccggt ggttaagttg ctgctcttgg gtggttgaat aaagcaagat ttgtcacccc
    61261 agcgtaaagt aagtttgctg tcatcctgaa tgccgttgag gtatacaaag ccgccatcag
    61321 caacaagtcc taattctttg tggctggttt catcttttac gctaatcccc agtggtgggt
    61381 gactaccatc tgccaggcga ataaccccca tgatttgata gcccttgcgg gtatcaagtt
    61441 tggcgtagcc aatagcacct tcggttaagg tggtggtgat aaccgagttt tcgatatcca
    61501 cattttctgg aagatttcgg gtatcaacgc ttaatgatgt tgttatgtaa ctgctgactg
    61561 atggaataac tccgatgcca taccgattag ttaccgcgcg tttattattc aacggaatat
    61621 cttcagcgcc gtctgcgtcg atcataaaac gcgagtcagc actaccacta tagtcatgga
    61681 aagcggcgcc gtggcgagtt gcagtgaatg aaccgttcca actggcattc agagaatagt
    61741 agctgtcccg ctgtgatgta ccggagagat agagacgacc gttctcggta ttatgctgat
    61801 aactggcgcg cagtgacgcc tccatatctt taaattcgtc attgtcgcct gaagcagaaa
    61861 cgctccagga attatttcga tcggaagagt cataccacga agcagtatgc gaaatgttgt
    61921 tatcctgatt tcgttgcata ccatagctca atgttctact ggttccccag ggaattgaaa
    61981 tatttaggta cagttgatcc tggttgtcat cttcccagtt tattcggcta aatgttagtg
    62041 aggtggatac gttttttaat gggccgatat caaaatcgcg gctaagtgat aatgaataat
    62101 tcacattact gctggcgtcc cagtaagtat tacgagccag acttacgtat gtatttaatc
    62161 tcaatgattc aagatactgg ttaaacgtga caatataact ctctttttca ttctgataat
    62221 tcgtgtaatg gttagtgtca ttgatatatt cattcatcga cacaaagttt ttatcagaga
    62281 aacggtagcc agcgaaagtg agctggctac cggtagattc aaaacgtttt gaatagttag
    62341 cacggtagct ataacccgtt tctttattct gattatgtaa ctgagcatca gatcgcgtga
    62401 catcaaagga taatgagccc aacgagttaa ggttaaaacc aactccggta gccagggatt
    62461 gataatcacg gtttgttaat actgaaccac catacaggga tacattgttc agccaacccc
    62521 atgagaattc accggtccag aagaagggct gttgtaagga atcgccgcca acgctggtcg
    62581 gtttccccat tgcagtagtg tagcgaatct gccctttacg cgtcagataa ggaatgctat
    62641 tagatgcaac ttgccattgc gtgacgcgcc cgtcctcttc ttcaaccttg acgtccagtt
    62701 gcccctgtaa tgtattgaat aaattggtaa tagaaaatgg gcctggagga acgttttctt
    62761 gataaataat acggttgttc tgtgagacag tgacctttgc attcgtttgt gcaacacccg
    62821 tgatttgcgg tgcgtaacca cgtagatcag gcggcaacat attctcatcg cttttcaacg
    62881 aaacgccaga aaaatggaaa gagtcgaaaa tagaggattc agtatcgtat tgacctatag
    62941 ttagttttgc attaattgct gggattgggc gaaacaaata aatacgcgga aaatccaggt
    63001 tgttcgcctg gcttttgcca ttgttaaatg actgatcgta ctggtaatca gtacgcaggc
    63061 gccaggcccc aagattaacc ccagcctgcc cataggaact tatattttgt gaattatcgc
    63121 cctgatgtgg tgcataatga gaggcatata aattgtagtc aagaaatgcc ccagcaatac
    63181 catgatccca aagttcagga ggcgtccagt ttgcatcttt gtattttaac catgcctgag
    63241 gtgcagatat ggataattgc attttccctt tatcgagata agttgtaatt tgtttttctt
    63301 tttcgatagg gtaacaacca tcgatcaggt tttgctgagc ctcttcaata atctcatcag
    63361 taaagcctaa taatgttaat tgctctggtg tcacgcaaac tgcagttagg ttatcaatta
    63421 atacccattg gaggtcaagg tggattggcg tcctcttatt attaatcaga gtgcttagtg
    63481 aatatttgcc gacgggtaat tggccatcag tttcaaattg ggaaaggtta acgttttcac
    63541 gatcttcggc ttcgattaaa tctttgttga attcaacggc ataagctgac gcacaaccaa
    63601 tgatgataac ggataatgaa caataaatat ttttaatcaa ttcgcttatt gtggttaatt
    63661 ttaatttttt atacatctga tccacttatc attaatc
//
LOCUS       DAJOSY010000013        63625 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJOSY010000013 DAJOSY010000000
VERSION     DAJOSY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.013"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            82..918
                     /gene="eutJ"
                     /locus_tag="OHN27_000899"
     CDS             82..918
                     /gene="eutJ"
                     /locus_tag="OHN27_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCQ2285394.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCLQPGVAELFRKQFPALQVHLPQHSLFMTPLAIAGSGREKAEGLY
                     AK"
     gene            908..2095
                     /gene="eutG"
                     /locus_tag="OHN27_000900"
     CDS             908..2095
                     /gene="eutG"
                     /locus_tag="OHN27_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCQ2285395.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            2312..3538
                     /gene="eutH"
                     /locus_tag="OHN27_000901"
     CDS             2312..3538
                     /gene="eutH"
                     /locus_tag="OHN27_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCQ2285396.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            3535..4938
                     /gene="eutA"
                     /locus_tag="OHN27_000902"
     CDS             3535..4938
                     /gene="eutA"
                     /locus_tag="OHN27_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCQ2285397.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            4950..6311
                     /gene="eutB"
                     /locus_tag="OHN27_000903"
     CDS             4950..6311
                     /gene="eutB"
                     /locus_tag="OHN27_000903"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCQ2285398.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            6332..7219
                     /gene="eutC"
                     /locus_tag="OHN27_000904"
     CDS             6332..7219
                     /gene="eutC"
                     /locus_tag="OHN27_000904"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCQ2285399.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            7229..7888
                     /gene="eutL"
                     /locus_tag="OHN27_000905"
     CDS             7229..7888
                     /gene="eutL"
                     /locus_tag="OHN27_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCQ2285400.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            7901..8401
                     /gene="eutK"
                     /locus_tag="OHN27_000906"
     CDS             7901..8401
                     /gene="eutK"
                     /locus_tag="OHN27_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCQ2285401.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSVGTLRKRSSR
                     YRLKPH"
     gene            8447..9499
                     /gene="eutR"
                     /locus_tag="OHN27_000907"
     CDS             8447..9499
                     /gene="eutR"
                     /locus_tag="OHN27_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCQ2285402.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRNQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSMTVKDAAMQWGFWHLGQFATDYQQLF
                     SEKPSLTLHQRMREWG"
     gene            complement(9505..10404)
                     /gene="hemF"
                     /locus_tag="OHN27_000908"
     CDS             complement(9505..10404)
                     /gene="hemF"
                     /locus_tag="OHN27_000908"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCQ2285403.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGADPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDRCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(10408..11277)
                     /gene="amiA"
                     /locus_tag="OHN27_000909"
     CDS             complement(10408..11277)
                     /gene="amiA"
                     /locus_tag="OHN27_000909"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCQ2285404.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDELLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            11491..11916
                     /locus_tag="OHN27_000910"
     CDS             11491..11916
                     /locus_tag="OHN27_000910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCQ2285405.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGDVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            11903..12352
                     /locus_tag="OHN27_000911"
     CDS             11903..12352
                     /locus_tag="OHN27_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCQ2285406.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            12413..12988
                     /locus_tag="OHN27_000912"
     CDS             12413..12988
                     /locus_tag="OHN27_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCQ2285407.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            13084..13983
                     /gene="yfeX"
                     /locus_tag="OHN27_000913"
     CDS             13084..13983
                     /gene="yfeX"
                     /locus_tag="OHN27_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCQ2285408.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEDNKEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(14223..15527)
                     /gene="pbp4b"
                     /locus_tag="OHN27_000914"
     CDS             complement(14223..15527)
                     /gene="pbp4b"
                     /locus_tag="OHN27_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCQ2285409.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYYQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMTIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(15532..16956)
                     /gene="murP"
                     /locus_tag="OHN27_000915"
     CDS             complement(15532..16956)
                     /gene="murP"
                     /locus_tag="OHN27_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCQ2285410.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(16960..17856)
                     /gene="murQ"
                     /locus_tag="OHN27_000916"
     CDS             complement(16960..17856)
                     /gene="murQ"
                     /locus_tag="OHN27_000916"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCQ2285411.1"
                     /translation="MQFEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            18020..18877
                     /gene="murR"
                     /locus_tag="OHN27_000917"
     CDS             18020..18877
                     /gene="murR"
                     /locus_tag="OHN27_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCQ2285412.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            19006..19797
                     /gene="ucpA"
                     /locus_tag="OHN27_000918"
     CDS             19006..19797
                     /gene="ucpA"
                     /locus_tag="OHN27_000918"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCQ2285413.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            19955..20971
                     /gene="cysP"
                     /locus_tag="OHN27_000919"
     CDS             19955..20971
                     /gene="cysP"
                     /locus_tag="OHN27_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCQ2285414.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            20971..21804
                     /gene="cysT"
                     /locus_tag="OHN27_000920"
     CDS             20971..21804
                     /gene="cysT"
                     /locus_tag="OHN27_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCQ2285415.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSINTLQSRFGRRVVG
                     H"
     gene            21804..22679
                     /gene="cysW"
                     /locus_tag="OHN27_000921"
     CDS             21804..22679
                     /gene="cysW"
                     /locus_tag="OHN27_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCQ2285416.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            22669..23766
                     /gene="cysA"
                     /locus_tag="OHN27_000922"
     CDS             22669..23766
                     /gene="cysA"
                     /locus_tag="OHN27_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCQ2285417.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            23900..24811
                     /gene="cysM"
                     /locus_tag="OHN27_000923"
     CDS             23900..24811
                     /gene="cysM"
                     /locus_tag="OHN27_000923"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCQ2285418.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(25000..25734)
                     /locus_tag="OHN27_000924"
     CDS             complement(25000..25734)
                     /locus_tag="OHN27_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCQ2285419.1"
                     /translation="MKKRFIYHDEKSNKFWWIDYEGDSLAVNYGKVGSIGKFQTKEFD
                     NEEQCLKEASKLIAAKMKKGYQEDPKFNFMDRYYFDDEEIGLHVKTSHPNFQCHFTDP
                     LYMCCWDEESPFGSDEGADALNVLENSLRKEPDLDCADFPQMLIETMWGMKYIAMDSI
                     LEEDVRAQLLVDEMSTIQSNMITYATAFGQIKVMGKISHKLKKMGLNALARHQLTAKI
                     LQWGDGQDSPILQKMIDDLTAFPHEN"
     gene            complement(25767..26141)
                     /locus_tag="OHN27_000925"
     CDS             complement(25767..26141)
                     /locus_tag="OHN27_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCQ2285420.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCYEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            26246..27097
                     /gene="pdxK"
                     /locus_tag="OHN27_000926"
                     /gene_synonym="thiJ"
     CDS             26246..27097
                     /gene="pdxK"
                     /locus_tag="OHN27_000926"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCQ2285421.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVVTSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(27140..27649)
                     /gene="crr"
                     /locus_tag="OHN27_000927"
     CDS             complement(27140..27649)
                     /gene="crr"
                     /locus_tag="OHN27_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCQ2285422.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(27690..29417)
                     /gene="ptsI"
                     /locus_tag="OHN27_000928"
     CDS             complement(27690..29417)
                     /gene="ptsI"
                     /locus_tag="OHN27_000928"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCQ2285423.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(29462..29719)
                     /gene="ptsH"
                     /locus_tag="OHN27_000929"
     CDS             complement(29462..29719)
                     /gene="ptsH"
                     /locus_tag="OHN27_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCQ2285424.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(30103..31074)
                     /gene="cysK"
                     /locus_tag="OHN27_000930"
     CDS             complement(30103..31074)
                     /gene="cysK"
                     /locus_tag="OHN27_000930"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCQ2285425.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(31259..32020)
                     /gene="cysZ"
                     /locus_tag="OHN27_000931"
     CDS             complement(31259..32020)
                     /gene="cysZ"
                     /locus_tag="OHN27_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCQ2285426.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            32250..33236
                     /gene="zipA"
                     /locus_tag="OHN27_000932"
     CDS             32250..33236
                     /gene="zipA"
                     /locus_tag="OHN27_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCQ2285427.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPSEAQVPPQHAPHPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            33307..35322
                     /gene="ligA"
                     /locus_tag="OHN27_000933"
     CDS             33307..35322
                     /gene="ligA"
                     /locus_tag="OHN27_000933"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCQ2285428.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            35324..35542
                     /locus_tag="OHN27_000934"
     CDS             35324..35542
                     /locus_tag="OHN27_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCQ2285429.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(35539..36537)
                     /locus_tag="OHN27_000935"
     CDS             complement(35539..36537)
                     /locus_tag="OHN27_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCQ2285430.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            36627..37553
                     /locus_tag="OHN27_000936"
     CDS             36627..37553
                     /locus_tag="OHN27_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2285431.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLSLRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(37592..38356)
                     /locus_tag="OHN27_000937"
                     /pseudo
     CDS             complement(37592..38356)
                     /locus_tag="OHN27_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38605..39438
                     /gene="xapA"
                     /locus_tag="OHN27_000938"
     CDS             38605..39438
                     /gene="xapA"
                     /locus_tag="OHN27_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HCQ2285432.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            39498..40754
                     /gene="xapB"
                     /locus_tag="OHN27_000939"
     CDS             39498..40754
                     /gene="xapB"
                     /locus_tag="OHN27_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HCQ2285433.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWAMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVTH"
     gene            40807..40965
                     /locus_tag="OHN27_000940"
     CDS             40807..40965
                     /locus_tag="OHN27_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HCQ2285434.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            41006..41890
                     /gene="xapR"
                     /locus_tag="OHN27_000941"
     CDS             41006..41890
                     /gene="xapR"
                     /locus_tag="OHN27_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HCQ2285435.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARIEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVDVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(41999..42124)
                     /locus_tag="OHN27_000942"
     ncRNA           complement(41999..42124)
                     /ncRNA_class="other"
                     /locus_tag="OHN27_000942"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(42155..42230)
                     /locus_tag="OHN27_000943"
     tRNA            complement(42155..42230)
                     /locus_tag="OHN27_000943"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42195..42197),aa:Lys,seq:ttt)
     gene            complement(42235..42310)
                     /locus_tag="OHN27_000944"
     tRNA            complement(42235..42310)
                     /locus_tag="OHN27_000944"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42275..42277),aa:Val,seq:tac)
     gene            complement(42357..42432)
                     /locus_tag="OHN27_000945"
     tRNA            complement(42357..42432)
                     /locus_tag="OHN27_000945"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42397..42399),aa:Val,seq:tac)
     gene            complement(42477..42552)
                     /locus_tag="OHN27_000946"
     tRNA            complement(42477..42552)
                     /locus_tag="OHN27_000946"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42517..42519),aa:Val,seq:tac)
     gene            42811..44226
                     /gene="gltX"
                     /locus_tag="OHN27_000947"
     CDS             42811..44226
                     /gene="gltX"
                     /locus_tag="OHN27_000947"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCQ2285436.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(44278..44670)
                     /locus_tag="OHN27_000948"
     CDS             complement(44278..44670)
                     /locus_tag="OHN27_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCQ2285437.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(44672..45031)
                     /locus_tag="OHN27_000949"
     CDS             complement(44672..45031)
                     /locus_tag="OHN27_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCQ2285438.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            45252..45327
                     /locus_tag="OHN27_000950"
     tRNA            45252..45327
                     /locus_tag="OHN27_000950"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45285..45287,aa:Ala,seq:ggc)
     gene            45367..45442
                     /locus_tag="OHN27_000951"
     tRNA            45367..45442
                     /locus_tag="OHN27_000951"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45400..45402,aa:Ala,seq:ggc)
     gene            45651..47840
                     /locus_tag="OHN27_000952"
     CDS             45651..47840
                     /locus_tag="OHN27_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCQ2285439.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVYCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(47890..49092)
                     /gene="nupC"
                     /locus_tag="OHN27_000953"
     CDS             complement(47890..49092)
                     /gene="nupC"
                     /locus_tag="OHN27_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCQ2285440.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            49428..50666
                     /locus_tag="OHN27_000954"
     CDS             49428..50666
                     /locus_tag="OHN27_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCQ2285441.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(50807..51133)
                     /locus_tag="OHN27_000955"
     CDS             complement(50807..51133)
                     /locus_tag="OHN27_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCQ2285442.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(51248..52504)
                     /locus_tag="OHN27_000956"
     CDS             complement(51248..52504)
                     /locus_tag="OHN27_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCQ2285443.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLTALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDDWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            52708..53673
                     /gene="glk"
                     /locus_tag="OHN27_000957"
     CDS             52708..53673
                     /gene="glk"
                     /locus_tag="OHN27_000957"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCQ2285444.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            53893..54219
                     /locus_tag="OHN27_000958"
     CDS             53893..54219
                     /locus_tag="OHN27_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCQ2285445.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            54241..55488
                     /locus_tag="OHN27_000959"
     CDS             54241..55488
                     /locus_tag="OHN27_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCQ2285446.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            55503..56588
                     /gene="ypdF"
                     /locus_tag="OHN27_000960"
     CDS             55503..56588
                     /gene="ypdF"
                     /locus_tag="OHN27_000960"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCQ2285447.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            56588..57625
                     /gene="ypdE"
                     /locus_tag="OHN27_000961"
     CDS             56588..57625
                     /gene="ypdE"
                     /locus_tag="OHN27_000961"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCQ2285448.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWVETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            57650..60145
                     /gene="ptsP"
                     /locus_tag="OHN27_000962"
     CDS             57650..60145
                     /gene="ptsP"
                     /locus_tag="OHN27_000962"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCQ2285449.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSCLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNKLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRLSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(60148..61005)
                     /locus_tag="OHN27_000963"
     CDS             complement(60148..61005)
                     /locus_tag="OHN27_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2285450.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRSVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(61018..61752)
                     /gene="ypdB"
                     /locus_tag="OHN27_000964"
     CDS             complement(61018..61752)
                     /gene="ypdB"
                     /locus_tag="OHN27_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCQ2285451.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(61767..63464)
                     /gene="ypdA"
                     /locus_tag="OHN27_000965"
     CDS             complement(61767..63464)
                     /gene="ypdA"
                     /locus_tag="OHN27_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCQ2285452.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT        14945 a        15962 c        17302 g        15416 t
ORIGIN      
        1 gggtaaccag cgcgcgtacg tttgtccgtc tgcgtcgctg tgtattagtc gatgcgtttc
       61 gcattgttta aggagtaaca gatggcgcac gacgaacaat ggctcacccc acgtctgcaa
      121 acggcggcaa cgctgtgtaa ccagacgccc gccgcgacgg aatctccgct gtggctgggg
      181 gtggatctgg gcacctgcga tgtggtgtcg atggttgttg accgcgacgg tcagccggtg
      241 gcggtatgcc tcgactgggc cgatgtggtc cgcgacggca tcgtctggga tttcttcggt
      301 gcggtcacca ttgttcgtcg ccatctcgac actcttgagc agcaattcgg tcttcgtttt
      361 agccatgcgg cgacttcatt tcctccaggt acggacccgc gtatttccat caacgtgctg
      421 gaatctgccg ggctggaagt gagccatgtg ctggatgagc caaccgcagt tgcggatctg
      481 ctgcaactgg acaacgccgg tgtagtggat atcggcggcg gcactaccgg cattgccatc
      541 gtgaaaaagg gcaaggtgac gtactcggcg gatgaagcca ccggcggaca tcacatctct
      601 ctgactctcg ccggaaatcg ccgtatttcg ctggaagagg cggagcagta caagcgcggt
      661 cacggtgaag agatttggcc tgcggtgaaa ccggtgtacg aaaaaatggc ggacattgtc
      721 gcccggcata ttgaagggca gggcattact gatttatggc tggcgggcgg ctcttgtttg
      781 caaccgggcg tggcggaact gtttcgcaaa caattcccgg cgttacaggt gcatttaccg
      841 cagcacagtt tgtttatgac cccgctggcg atcgccggta gtgggcgaga gaaagcggag
      901 gggctatatg caaaatgaat tgcagaccgc gctctttcag gcgttcgata ccctgaatct
      961 gcaacgggta aaaacattta gcgttccacc ggtgacgctt tgcggtccgg gcgcggtgag
     1021 cagttgcggg cagcaagcgc aaacgcgtgg gctgaaacat ctgttcgtga tggcagacag
     1081 ctttttgcat caggcgggga tgaccgccgg gctgacgcgc agcctggctg ttaaaggcat
     1141 cgccatgacg ctctggccat gtccggtggg cgaaccgtgc attaccgacg tgtgtgcagc
     1201 cgtggcgcag ttgcgtgagt caggctgtga tggggtgatc gcatttggcg gcggctcggt
     1261 gctggatgcg gcgaaagccg tggcgttgct ggtgacgaac cccgatagca cgctggcaga
     1321 gatgtcagaa accagcgttc tgcaaccgcg cttgccgctg attgccattc caacgaccgc
     1381 cggaaccggc tctgaaacca ccaatgtaac ggtgattatc gacgcggtga gcgggcgcaa
     1441 gcaggtgtta gcccatgcct cgctgatgcc ggatgtggcg atcctcgacg ccgcattgac
     1501 cgaaggtgtg ccgtcgcatg tcacggcgat gaccggcatt gatgcgttaa cccatgccat
     1561 tgaagcatac agcgccctga acgctacacc gtttaccgac agcctggcga ttggtgccat
     1621 tgcgatgatt ggcaaatcgc tgccgaaagc ggtgggctac ggtcacgacc ttgccgcgcg
     1681 cgagagcatg ttactggctt catgtatggc gggaatggcg ttttccagtg cgggtcttgg
     1741 gttgtgccac gcgatggcgc atcagccggg cgcggcgctg catattccgc acggtctcgc
     1801 gaacgccatg ttgctgccaa cggtgatgga atttaaccgg atggtttgtc gtgaacgctt
     1861 tagtcagatt ggtcgggcac tgcgaactaa aaaatccgac gatcgtgacg ctattaacgc
     1921 ggtaagtgag ctgattgcgg aagttgggat tggtaaacga ctgggcgatg ttggtgcgac
     1981 atctgcgcat tacggcgcat gggcgcaggc cgcgctggaa gatatttgtc tgcgcagtaa
     2041 cccgcgtacc gccagcctgg agcagattgt cggcctgtac gcagcggcgc aataaatgcc
     2101 ggatgcgacg cttgccgcat cttatccagc ctacgggatt gcacatgtag ggcggataag
     2161 gcgttcacgc cgcatccgcc agtggcgcgg tgcaattgcc ggatgcgacg cttgccgcgt
     2221 cttatccagc ctacgggatt gcacatgtag ggcggataag gcgtttacgc cgcatccgcc
     2281 aataaataac aactaacagg gagtaaaggc gatgggaatt aacgaaatca tcatgtacat
     2341 catgatgttc tttatgctga tagctgccgt agacaggatc ctgtcgcagt tcggcggttc
     2401 tgctcgtttc ctcggtaagt tcggtaaaag tatcgaagga tcaggcggtc agttcgaaga
     2461 aggctttatg gcaatgggcg cactgggcct ggcgatggtc ggtatgaccg cgctggcacc
     2521 ggtactggct cacgttctcg ggccggtaat tattccggtt tacgaaatgc tcggcgctaa
     2581 cccatcgatg ttcgccggaa cactgctggc gtgcgatatg ggcggcttct tcctcgccaa
     2641 agagctggcg ggcggcgacg tagccgcgtg gctatactct gggttaattc tcgggtcgat
     2701 gatggggcca acgattgtgt tttccattcc ggtggcgctc ggcattatcg aaccttctga
     2761 ccgtcgttat ctggcgctcg gcgtgctggc gggcattgtg accattccga ttggttgtat
     2821 cgctggtggt ctggttgcta tgtactccgg tgtgcagatc aacggccagc cggtggaatt
     2881 cactttcgcc ctgatcctga tgaacatgat cccggtgatc attgttgcga ttctggtggc
     2941 gctggggctg aaattcatcc cggaaaaaat gatcaacggc ttccagatct tcgccaaatt
     3001 cctcgttgca ttgatcaccc tcggtcttgc cgctgcggta gtgaaattcc tgcttggctg
     3061 ggaactgatc cccggtctgg atcctatctt tatggcccct ggcgataaac ccggtgaggt
     3121 gatgcgcgcc attgaagtta tcggttctat ctcctgcgtt ctgttagggg cgtatccgat
     3181 ggtgctgctg ctgactcgct ggtttgaaaa accgctgatg agcgtcggta aagtactgaa
     3241 tatgaacaac atcgcggcag ccggcatggt ggcaacgctt gccaacaaca tcccgatgtt
     3301 cggcatgatg aagcagatgg atacccgcgg caaagtcatc aactgcgcct tcgccgtttc
     3361 cgctgctttc gccctgggcg accacttagg cttcgccgct gccaacatga acgccatgat
     3421 cttcccgatg attgtcggca agttgatcgg cggcgtaacg gcgattggcg tggcgatgat
     3481 gctggtgcca aaagaagacg cgaccgcgac taaaaccgaa gcggaggcac aatcgtgaac
     3541 actcgccagc tattgagcgt cggtatcgat atcggcacca ccaccaccca ggtgattttc
     3601 tcccacctgg agctggttaa ccgtgcggcg gtgtcgcagg tgccgcgcta cgaattcatc
     3661 aaacgcgaaa ttagctggca aagcccggtg ttctttaccc ctgtcgataa acagggtggt
     3721 ttaaaagaag cggaactgaa atcgctgatt ttagagcaat atcaggctgc gggcattgcg
     3781 ccggaaagcg ttgattctgg tgccatcatc atcaccggtg aaagcgcgaa aacccgcaat
     3841 gctcgcccgg cggtgatggc gctctctcag tcgctgggcg attttgtcgt cgccagcgcc
     3901 gggccgcacc tcgaatccgt gatcgccggt cacggagccg gggcgcaaac cctttctgaa
     3961 caacggctgt gtcgggtact gaatatcgac atcggcggtg gcaccgcgaa ctacgccctg
     4021 ttcgatgccg gaaaaatcag cggcactgcc tgtctcaacg tcggtggtcg cctgctggaa
     4081 accgacagcc aggggcgcgt ggtttacgct cataaaccgg ggcagatgat tgtggatgag
     4141 tgcttcggtg caggcactga cgcccgttcg ctgaccggcg cgcagctggt gcaggttacc
     4201 cggcggatgg cggcgctgat tgtcgaagtg attgacggaa cgctttcgcc gctcgcgcag
     4261 gcattgatgc aaaccggttt gctgcccgca ggtgttacgc ccgaaatcat tacgctttct
     4321 ggaggcgtgg gcgaatgtta tcgccaccag cccgccgacc cgttctgttt tgccgatatt
     4381 ggcccacttc tggcaacggc gctgcatgac catccgcgcc tgcgtgagat gaatgtgcag
     4441 tttccggcgc aaaccgtacg cgccacggtg attggcgcgg gtgcacatac cctttcgctc
     4501 tctggcagca caatctggct ggagggcgta caactgccgc tgcgcaattt gccggtggcg
     4561 atcccgattg atgaaacgga tctggtgagt gcctggcaac aggcgctgct tcagctggat
     4621 cttgatccca aaactgacgc gtacgtgctg gcgcttcccg cctcgctgcc tgtgcgttac
     4681 gccgcggtac tgacggtcat caacgcgctg gtcgatttcg tcgcgcgttt tccgaatccg
     4741 catcccctgc tggtggtggc cgggcaggac tttggtaaag ctctgggcat gttgttgcgc
     4801 ccacagctac aacaactccc gttggcagtc attgacgaag tgattgtccg cgcgggggac
     4861 tatatcgaca ttggtacgcc tctttttggc ggatcggttg tgccggtgac ggtgaaatca
     4921 ctcgcatttc cttcctgagg gaacgactta tgaaactaaa gaccacattg ttcggcaatg
     4981 tatatcagtt taaggatgta aaagaggtgc tggctaaagc caacgaactg cgttcggggg
     5041 atgtgctggc gggcgttgca gcggcaagct cacaggagcg cgtggcggca aagcaggtgt
     5101 tgtcggaaat gaccgtagcg gacatccgca ataatccggt gattgcctat gaagatgact
     5161 gcgtgacgcg gctgattcag gacgacgtta acgaaacggc ctacaaccag attaaaaact
     5221 ggagcatcag cgaactgcgt gagtatgtgc tgagcgatga aaccagcgtg gacgacattg
     5281 cctttacccg caaagggctg acctcggaag tggtcgcggc ggtagcgaag atttgctcca
     5341 acgcggacct gatctacggc gcgaagaaaa tgccggtaat caaaaaggcc aataccacca
     5401 tcggtattcc gggcaccttt agcgcccgtt tgcagccgaa cgatacccgt gacgacgtgc
     5461 aaagtatcgc tgcgcaaatc tatgaagggc tttcctttgg ggtgggcgat gcggtgatcg
     5521 gcgttaaccc ggtaactgac gacgtggaaa acttaagccg cgtgctggat accatttatg
     5581 gcgtgatcga caaattcaac atcccaactc agggctgcgt actggcgcac gtcaccaccc
     5641 agatcgaagc gattcgtcgc ggcgcgcctg gcggactgat tttccagagt atctgtggca
     5701 gcgaaaaagg gctgaaagag tttggcgtgg aactggcgat gctcgacgaa gcgcgcgcag
     5761 tgggcgcaga gttcaatcgt atcgccgggg aaaactgcct ctacttcgaa accggacaag
     5821 gctctgcgct atccgctggc gctaacttcg gcgctgacca ggtgacgatg gaagcacgta
     5881 actatgggct ggcgcgtcat tacgatccgt ttatcgtcaa caccgtggtc ggctttattg
     5941 ggccggagta tctctacaac gaccgccaga ttatccgtgc tggcttagaa gatcacttta
     6001 tgggcaagct gagcggcatc tctatgggct gtgactgctg ctacaccaac cacgctgacg
     6061 ctgaccagaa cctcaacgaa aacctgatga tcctgctcgc caccgcaggc tgcaactaca
     6121 tcatggggat gccgctgggt gatgacatca tgctcaacta ccagaccacc gcattccacg
     6181 ataccgccac tgtgcgtcag ttactcaacc tgcgtccgtc accggagttt gaacgctggc
     6241 tggaaagcat gggcattatg gcaaacggtc gcctgaccaa acgggcgggc gatccgtcac
     6301 tgttcttctg atgacgcgga gataacacat catggatcaa aaacagattg aagaaattgt
     6361 acgcagcgtg atggcgtcaa tgggacaaac ggcccccgcg ccgtcagaag caaagtgcgc
     6421 caccaccaac tgtgcggcac cggtgacctc ggaaagctgt gcgctggatt taggttccgc
     6481 tgaagcaaaa gcgtggattg gtgttgaaaa cccgcatcgc gcagacgtat taacagaact
     6541 gcgccgcagc accgtggccc gcgtttgtac cggtcgtgcc ggtccgcgtc cacgtaccca
     6601 ggcgctgctg cgtttcctgg ccgatcactc tcgttcgaaa gataccgtac tgaaagaagt
     6661 gccggaagag tgggtgaaag cgcagggctt gctggaagta cgctctgaaa ttagcgacaa
     6721 aaatctctac ctgactcgcc cggatatggg ccgccgcctg tgtgcagaag ctgttgaagc
     6781 gctgaaagca cagtgtgttg ctaatccgga cgtacaggtc gttatttctg atggcctgtc
     6841 aactgatgcc atcaccgtca actacgaaga gatcctgccg ccgctgatgg cgggcctgaa
     6901 acaggccggg ctgaaagtcg gcacgccatt ctttgttcgt tatggtcgcg tgaagattga
     6961 agatcagatc ggtgagatcc tcggcgcgaa agtggtgatc ctgctggtgg gggaacgtcc
     7021 ggggttaggg cagtcagaaa gcctctcctg ctacgccgtt tactcgccgc gtatggcgac
     7081 caccgttgag gccgatcgca cctgtatctc taacattcac cagggcggca cgccgccggt
     7141 tgaagccgca gccgtcattg tggatttggc gaaacgtatg ctggagcaga aagcatccgg
     7201 catcaacatg acccgataag gaggcatcat gccagcttta gatttgattc gaccgtcggt
     7261 aaccgccatg cgggtgattg cctccgttaa cgctgaattt gcgcgtgaac taaaattgcc
     7321 gccgcatatt cgtagcctcg ggctgatttc tgccgattct gacgacgtca cctacattgc
     7381 ggctgacgaa gcgaccaaac aagcgatggt cgaagtggta tatggtcgct cgctgtatgc
     7441 gggagcggca cacggcccgt caccgactgc cggtgaagtg ctgattatgc ttggtgggcc
     7501 aaacccggcg gaagtgcgtg ccggtctgga tgcgatggtt gcacatattg aaaatggcgc
     7561 agctttccag tgggccaatg acgcagaaaa caccgccttc ctggcacatg tagtttcgcg
     7621 taccggttct tatctctcat caaccgccgg gatcacgctt ggcgatccga tggcgtatct
     7681 ggtggcaccg ccgctggaag cgacctacgg cattgatgca gcgttgaaat ctgccgacgt
     7741 acagctggtg acctatgtcc cgccaccgtc tgaaaccaac tactcggcag catttttaac
     7801 cggtagccag gccgcgtgta aagcagcctg taacgccttt accgatgcag tgctggaaat
     7861 cgcgcgtaat ccaatccagc gtgcgtaacg gaggttgccg atgatcaatg cactgggatt
     7921 gctggaagtg gacggcatgg tcgccgcgat agatgcagcg gatgccatgc tcaaagcagc
     7981 taacgttcgt ctgctcagtc acgaagtgct tgaccctggt cgcttaacgc tggtggtgga
     8041 aggcgatctg gcggcgtgtc gtgcggcgct ggacgcaggt tgtgctgccg cgatgcgtac
     8101 cgggcgtgtc atcagccgca aggagatcgg tcggccagac gatgacaccc agtggctggt
     8161 tactggcttt aaccgccagc cgaagcaacc cgtaagggaa cccgacgcgc cagttatcgt
     8221 cgcggaatct gctgacgagt tgttggcgct gttaacatca gtacgtcagg gaatgacggc
     8281 aggagaagtg gctgcccact ttggctggcc gctggaaaaa gccagaaatg cgctcgaaca
     8341 gctcttttct gtcgggacgt tacgtaaacg cagtagtcgt tatcgtctca agccccatta
     8401 acctgtcgga ggtgccgggt gtcctacaac acccggcatt aacatcatga aaaagacccg
     8461 tacagccaat ttgcaccatc tttatcatga acccttaccc gaaaacctga agctcacgcc
     8521 gaaggtcgaa gtggataatg ttcatcaacg acagacaacg gatgtctatg aacatgcttt
     8581 aacgattacc gcctggcagc agatttacga tcagctgcat ccgggcaagt ttcatggtga
     8641 atttacggaa attctactcg atgatattca ggtttttcgt gaatacaccg gtctggcgct
     8701 gcgtcagtcg tgcctggtct ggccgaactc gttctggttt ggcattccgg cgacgcgcgg
     8761 tgagcaggga tttatcggtt cgcaatgtct gggaagcgcg gaaatcgcca cccgccctgg
     8821 tggcactgaa tttgaactga gcacgccgga tgattacacg atcctgggcg tggtgctttc
     8881 tgaagatgtc atcacccggc aggctaactt tttgcataac ccggatcggg tattacatat
     8941 gttgcgtaac cagtcggcgc tggaagtgaa agagcagcat aaagccgcgc tgtggggctt
     9001 tgtccaacag gcgctggcga cgttttgcga gaatccggaa aatctccatc agcctgcagt
     9061 gcgaaaagtg ctgggggata atttgctaat ggcgatgggg gccatgctgg aagaagcgca
     9121 accaatggtg acggcggaaa gcatcagtca tcagagttac cgtcgattgc tttcccgcgc
     9181 ccgtgaatat gtgctggaaa acatgtccga accggtgacg gtgctggatt tgtgtaatca
     9241 actgcatgtc agccgccgca cgctacaaaa cgcgtttcac gctattttag gcattggccc
     9301 gaacgcgtgg ctgaaacgca ttcgcctgaa cgccgtacgc cgcgaactga taagtccgtg
     9361 gtcgcaaagt atgacggtaa aagacgccgc catgcagtgg ggattctggc atctggggca
     9421 atttgccacg gattaccagc agctgttttc cgagaagccg tcactgacgc tgcatcagcg
     9481 gatgcgggag tgggggtgag ggagttacac ccaatccctg accttaataa actcacttaa
     9541 cgccgcttct gggctgccat cttttggctg ataatcatat tcccagcgta ccagtggcgg
     9601 cattgacatc aggatagact cggtgcgccc gccagtttgc aggccaaaca gcgtgccgcg
     9661 atcccagacc agattgaact cgacataacg accgcgacga tataactgga aattgcgctc
     9721 gcgctcgccg taggccatcg ctttccgtcg ctcgacaatt ggtaaataag cgtcggtgta
     9781 gcctttgcct accgcctgca taaaggcaaa acagcggtcg aaatctggcg tgttcaggtc
     9841 atcaaagaac agcccgccaa taccgcgctg ttcgttgcga tgtttgaggt agaagtattc
     9901 gtcgcaccac tttttgtaac ggggataaac gtcttcgcca aatggcaggc acaggtcacg
     9961 ggcggtgcga tgccagtgaa tagcatcttc ttcaaaacca tagaatgggg ttaagtcgaa
    10021 gccaccgcca aaccaccaga cgggatcggc acccggtttt tcggcaataa aaaaccgcac
    10081 attcgcgtgg ctggtgggaa catacgggtt atgcggatgc actaccagtg aaacgcccat
    10141 cgcctcgaaa ctgcgcccgg caagttccgg gcgatgagcg gtggcggaag caggcatcgc
    10201 ctcaccgtgg acatgcgaaa agttgacgcc tgcctgttcg aaaacaccac cattacgcaa
    10261 cacccgacta cgcccgccgc cgccagcttc gcgctgccaa ctatcttcga caaattctgc
    10321 gccatcgacg gcggtcagct gctgacaaat cgtatcctga aggttgagca gaaactgttt
    10381 aacctggtgt gcgtcgggtt tcataactta tcgctttttc gaatgtgctt tctggttgtc
    10441 gaaccagtgg aaataactga tcacgccttc agcaatcgct gtggcgattt tctgacgaaa
    10501 cgccgccgtg cctaacagcc gttcttcttc cgggttggtg ataaacgagg tttccaccag
    10561 caccgaagga accgacggtg atttcaacac cacaaatgcc gcttgttcgg tgttgcggct
    10621 gtgcagttta tgcaccggct taatcttctt cagaatatgc gagccgagcg tcagactatt
    10681 tttaatggta tctgtttgca ccagatcaaa cagcacttgt tgcaataggt gatccttgtc
    10741 agtcgccttt ttaccggcaa cttcatcggc gcggttttcg cgttcagaca ggtatttcgc
    10801 cattgcgcta cttgccccac ggttagagag ggcaaatacc gaagcaccgg cagctttcgg
    10861 gttggtaaag ccatcggcat gaattgacat aaacagatct gcgccatgtt tatgggcgat
    10921 ttcaacgcga tcgtaaagtg ggataaacgt atcgccagaa cgcgttaaac gcgcatcaat
    10981 cccatgatta cgcaaaatgg aacggacgtt tttagcaatc gccagcacca catgtttttc
    11041 tttcgaaccg ttgcgtccga tcgctccggt atcaattccg ccgtgacctg gatcgagaac
    11101 aacgacacgt ttgccgccag attttttggc tttcggcttg ctgtgtccgt tgctggtttt
    11161 taaaagttcg tctttggcga tggcttgcga cattcctgac aacgtcaggg cagccaaacc
    11221 ggctttcagc acttggcggc gcgaagtgag tgtttttagt ggtttaaaag tgctcatacg
    11281 gcctgagttg taataataaa gttccagatg ttatatcgta tcgcgtaatc cgttacgacg
    11341 gtttgctgtt gagaattgtt ttacttttca tttcaatagt tgacaactcc ccattatgcc
    11401 actttttcaa ccgattttcg tcagatattg gctaattcga ccgttcacgc cataatcagc
    11461 cttttaaccc gcaaaaaggt aacgaatacc atggagatac gcgtatttcg ccaggaagat
    11521 ttcgaagagg tcatcaccct ttgggagcgt tgcgacttgc tgcgtccgtg gaacgatccg
    11581 gaaatggaca tcgagcgtaa gatgaaccat gacgtcagtt tgtttttggt cgctgaggta
    11641 aacggtgacg tggtcggaac ggtgatgggc ggttatgacg ggcatcgcgg gtctgcttat
    11701 tatcttggcg tgcatccaga gtttcgtggg cgtgggattg ccaatgcgtt gcttaatcgg
    11761 ctggagaaaa agctgattgc tcgtggctgc ccgaaaattc agatcaatgt gccggaagat
    11821 aacgacatgg tgctcggaat gtatgaacgc ctgggatatg aacacgccga cgtgctgagt
    11881 ctgggtaagc gtttgattga agatgaagag tactgagttt catcctgtcc attatgatgc
    11941 gcacggtcgc ctgcgtttac ccttgctctt ctggcttgtg ctattgcttc aggcgcgaac
    12001 ctgggtgctg tttgtcattg ccggtgcgtc gcgcgagcag ggtactgcgc tgctaaatct
    12061 gttttatccc gatcacgata atttctggct ggggcttatt cctggcattc cggcggtgct
    12121 ggcgtttttg ttgagcggtc ggcgggctac gtttcctcgc acctggcgtg tactctattt
    12181 tctgttgctg ttggcgcagg tggttttact ttgctggcaa ccgtggctgt ggctgaacgg
    12241 tgaatccgtt agcggtatcg gtctggcgct ggtagtggcg gatattgtgg cgttaatctg
    12301 gctgctgacc aatcgacgtt tacgcgcttg ttttaatgaa gaaaaagaat aacggcactt
    12361 tttggtgaat ttgcactcca agcaacgtta ttgaataacc aaaggaagtg acatgaaatc
    12421 gctgcgttta atgttatgcg ctatgccatt gatgctgacc ggctgttcca cgatgtcgtc
    12481 agttaactgg tctgccgcta acccgtggaa ctggtttgga tcgtccacca aagtgagcga
    12541 gcagggcgtg ggtgaattaa cggcgtccac accactgcaa gaacaagcca ttgccgatgc
    12601 gcttgatggc gattatcgcc tgcgcagcgg aatgaaaacc gcgaacggca acgtggtgcg
    12661 cttttttgaa gtgatgaagg gcgacaacgt ggcgatggtg attaacggcg atcaaggcac
    12721 gatcagtcgc attgacgtgc tggatagcga tattcctgct gacaccggtg ttaaaatcgg
    12781 tacaccgttt agcgaccttt acagcaaagc atttggcaat tgccaaaaag ctgatggtga
    12841 tgataatcgt gccgtcgaat gtaaagccga aggcagtcaa catattagct accagttcag
    12901 cggggaatgg cgtggtcctg aagggttaat gccttcggac gataccctga aaaactggaa
    12961 agtcagtaaa attatctggc ggcgttaatt tgcgtctgaa caaaccgccg ccgcgaaaaa
    13021 acacgtaaaa tagcgctcaa caatgccacg gattgcgtgg cattattatt ttcaggagga
    13081 acaatgtctc aggttcagag tggcattttg ccagaacatt gccgcgcggc gatttggatc
    13141 gaagccaacg tgaaagggga agttgacgcc ctgcgtgcgg ccagtaaaac atttgccgac
    13201 aaactggcaa cttttgaagc gaaattcccg gacgcgcatc ttggtgcggt ggttgccttt
    13261 ggtaacaaca cctggcgcgc tctgagcggc ggcgttgggg cagaagagct gaaagatttt
    13321 ccgggctacg gtaaaggcct tgcgccgacg acccagttcg atgtgttgat ccacattctt
    13381 tctctgcgtc acgacgtaaa cttctctgtc gcccaggcgg cgatggaagc ctttggtgac
    13441 tgcattgaag tgaaagaaga gatccacggc ttccgttggg ttgaagagcg tgacctgagc
    13501 ggcttcgttg acggtactga aaacccggcg ggtgaagaga cgcgccgcga agtggcggtt
    13561 atcaaagacg gcgtggatgc gggcggcagc tatgtgtttg tgcagcgttg ggagcacaat
    13621 ctgaagcagc tcaaccggat gagcgttcac gatcaggaga tgatgatcgg gcgcaccaaa
    13681 gaagataaca aagagatcga cggcgacgaa cgtccggaaa cctctcacct cacccgcgtt
    13741 gatctgaaag aagatggcaa agggctgaag attgttcgcc agagcctgcc gtacggcacc
    13801 gccagtggca ctcacggtct gtacttctgc gcctactgcg cgcgtctgca taacattgag
    13861 cagcaactgc tgagcatgtt tggcgatacc gatggtaagc gtgatgcgat gttgcgtttc
    13921 accaaaccgg taaccggcgg ctattatttc gcaccgtcgc tggacaagtt gatggcgctg
    13981 taagcgtttc tgattcattg attaaaggcc aggcagtaac attactgact ggcctcagat
    14041 tgttgaccaa gtgcgcgttg tacacgccgg atgcggcgtg aacgccttat ccggcctacg
    14101 aaatcgtgct aattcaatat attgtgggct ccacgtaggc ctgataagcg tagcgcatca
    14161 ggcagttttg cgtttgtcag cactctcaga ccagccagta acattactga ctggcctttt
    14221 tattacttct gctttaacgc cgcatacacc tgatcaacta cccaaccata agttgcaatc
    14281 ggcaactgac cgctttcgaa catattggga ttcttttgcg gatcggcaac cggcgaatgt
    14341 ggcttgttgc ttaacatcac aatcgtcata tgattcaccg gatcgataac ggtcaccgtt
    14401 ccggtccagc cagtgtgacc gtaggtttgc gggcttgcca gcgtgccaaa cgtcggcgtc
    14461 atggtggcat taccattcac gcgccagccg aggccaaaag tggcatcttc cttagagctg
    14521 gtggtgaaca tcttcaccgt ttccgcattg aacagctgca catcaccata gcccccgccg
    14581 ttcagcatcg tttgcattaa caccgcaata tcgccggtat tggaaaacaa acctgcgtgc
    14641 ccggaaacgc cgcccatcga ataaaaggct ttttcatcgt gcacctgacc ccagagagtg
    14701 gaggtgcgga tattcgggaa atggatcacg ccatcgcggg tattgccgtt caattccgtg
    14761 gcggcaattt gttgcggttt aaagcctttc agcagcgggt taaataccgt atgtgtcagg
    14821 ccgagcgggc gataaatcga ctcttcaaca tagcggtcaa gcggctgacc ggtgaccgac
    14881 tcgacgataa atccaagcag catataatcg acatcactgt aaatatgctt gctgccgggc
    14941 tgatactcta gcggcgtgcg cttgatcatc tccagcgtct ggcctttatc ctggtaatat
    15001 aacgcgcccg cgaccgcttt attcgggtat tgcggatctg ccgggaaacc gccgctgtga
    15061 tgcagcaggt cagaaatccg cagggtgttt ttgcctttaa tggtgtcatt tggactatcg
    15121 gcaaaccccg gaatatattt cgcaatccga tcgtcaggat gcagtttgcc ttcgctcatc
    15181 agcttttgca gggcgaagtt cgtggcgtac attttggtgt ttgaggccag gtcatacagc
    15241 gtcccggtgg tggctttgac aggctgttcc atcaacacgc tgccatcgta ctttttcgcc
    15301 gctccccagg ctttgcgata cacaatctga ttatctttaa tgatcagcag gtttacgccg
    15361 ggataaccga catcaacttg ctggctaatc cagcgatcca tctggttaag ccgttcgacg
    15421 ttaaaccctg ctttctctgg cgagctttct gtcagaacag ggtattttgc ggcgctgacg
    15481 ctacaactaa cagccagcag agaaagatag agcattgtcc gtttcatgat atcagtccag
    15541 attgacgtta cggcagccaa agagcgtggt gaaaataaac ccgcaaaccc atgccaccag
    15601 aatcccgcca gcataaaccg ccatggccgg aaggatgcct tgtgcggaag tcatcagcgg
    15661 cagcgctacc agaccagacg gcccaaaggc gctgtttaag cccatcggca gaccccacca
    15721 ggctatcagg ccgataaaca aaccgcccgc cgcgccgcct aaacaggcgg taacaaacgg
    15781 cttcatgcgg ggcagggtga caccgtaaat cagcggttca ccaacgccca gcaggccggg
    15841 aataatcgcc ccgcgtacct gactgcgtag cgcactgtgc ggttgcgccc gccagtagag
    15901 tgccagcgcc gcgcccacct ggcccgcgcc tgccattgaa aggatgggaa ataagctgtt
    15961 gaatccctgg ctgtccatta acgcgaggta aacaggaata aagccctgat gcacgccaaa
    16021 taccacggcg atcaggaaca gcccggctaa aaccgcacaa ccgaacggat tactgttcag
    16081 gtgcataaac agccatgaca taccttcgaa taaccagcca cccagcggca taatgatcag
    16141 gtacgcaagc gtagcggtga tcagcagggt gattaacgag gtcagcagca tgtcgagatc
    16201 gtccggcata aagcggcgca ccatgccttc aatgcgggcg caggcccacg cggcaatcag
    16261 cacgccgata atattgccgc gcggatcgat gggcagacca aagaaatcgt gaaaaccggc
    16321 gtagtaaccg gtggtcgctg cggggttata accgagcaaa aagagcgcgg cgataattgc
    16381 gccatttacg cccgtgccgc caaatgcctg ggcggcgtta tagcccacca gaatcaccag
    16441 gaaagtgaac aaacctttgc tgaacacctt cataaaattc agcgcatcgg gtagtgttcc
    16501 ctgagcatct gccggaacgt gcatcaccgt ggcaattaac gtcgctatcc ccagcagcag
    16561 accggcggca ataaaaccgg ggatcagcgg cgtgaagatg gtggcgaatt tggcgagaaa
    16621 ttgttgcacg ccggaggttt gtttggcttt taactgacgt ttgttctggg cggcgatttc
    16681 tgcggcatcc tgtaccggtg cctcgcccag cagctcgctc atcgcttttg cggcacgatg
    16741 tgctttacca ggcccaaaaa cgacctgcac ctggtcgctg gtcaaaatga cgcccttcac
    16801 gccttccagc gttttgatat tgggatcaac cagtgaactg tcatgtacac ccagacgcag
    16861 gcgcgtcata cagttaccac aactggcgat atttcccggt ccgccgacac gggtaagaat
    16921 ggtgttcaga agttcactgc tgatctcttt ggccatgggt tattccttgt ctaaaacctg
    16981 acgaataaag ccgccgtgtt gatccaggcg ttttttagct tctgcggcat cgagattttt
    17041 cagcaccatc acaatggccg ttttacagtt gcgctcgcaa gcaattaacg ccgcttccgc
    17101 ttgctctgcg ctacatccgg tggcgttttt aacaatattg acctggcgca catgcagttt
    17161 ttcgttggtg gcgaccacat cgaccatcag gttgccgaac actttgccgg atttaatcat
    17221 cagcccggtg gaaagcatat tgagcaccag tttctgcgct gtacctgctt tcatccgcga
    17281 agaaccggta acaacttcgg cacctacaat cggtgtaatg gcaaactcag cggtggttga
    17341 aacggcgctc cccggattac aggaaattcc cactgtgcgg cagccgagct ggcgtgcgta
    17401 ttccagtccg gcaatcacat acggcgtgcg accgctggca gcaatgccaa ccaccacatc
    17461 ctgtgccgtt aaattaatat ttttcagatc attaacaccg ccttcccggc tatcttccgc
    17521 gccttccacc gcgtgctgaa tggcatattc gccgccagca atcaaaccaa ccaccagacc
    17581 cggtttcacg ccgtaggtgg gcggacattc gctggcatcc agaatcccca gacgaccgga
    17641 tgttcccgca ccgaggtaaa tcagacgccc gccgccgctt acctgggcgt ggataacatc
    17701 gatcgccgcg gcgatatccg gcagtacgcg ctcaacggca agcggtacgg ttttatcttc
    17761 atcgttgata atccggcaca tttccagcgt cgatacgcgg tcaatttcag ccgaggcggt
    17821 gttcgagcct tcagtaatca tcttttcaaa ttgcatcggt ggtgacctta ctatttacga
    17881 tcaaataatt gcagatgtat ggaatcatat atcccgaatt gacagactta aaggaatcat
    17941 tgagtctaca ctcgcagagt gtgaagagcg tcataagaac ttaagcggaa tttttgctgc
    18001 cagcccagga gagtggaaga tgttgtacct gacgaaaata cgcaatgcgg aaagtgaatt
    18061 taccgagaac gagcaaaaaa ttgctgactt tctgcgggcc aacgtgagtg agctgcaatc
    18121 cgtttcttca cggcagatgg cgaaacagct cggcattagc cagtcgagca tagtgaaatt
    18181 tgcccaaaaa ctcggcgcgc aaggatttac tgaattgcgt atggcgttaa taggcgaata
    18241 cagtgccagc cgggagaaaa cgaatgccac ggcgctgcac ctgcacagtt cgattaccag
    18301 tgatgattcg ctggaagtga ttgcccgcaa actgaatcgc gaaaaagagc tggcgctgga
    18361 gcaaacctgt gcgttgttcg actacgcgcg gctgcaaaaa atcattgagg tcatcagtaa
    18421 agcaccgttt atccagataa cgggcctggg cgggtcggcg ctggtggggc gcgatctgtc
    18481 gttcaaactg atgaaaattg gctatcgcgt ggcctgtgaa gccgacacgc acgtacaagc
    18541 caccgtttcc caggcgttga aaaaaggcga tgtacaaatc gctatctctt acagcggcag
    18601 taaaaaagag attgtcttgt gcgcagaagc ggcacgaaag cagggggcaa cggtgattgc
    18661 cattacttct ctggctgact ctccgctgcg gcggctggcg catttcacgc tcgataccgt
    18721 ttccggtgaa accgaatggc gtagctcgtc gatgtctacc cgcacggcgc aaaattctgt
    18781 taccgattta ctgtttgttg gtcttgtgca actcaatgat gtggagtcat tgaaaatgat
    18841 tcagcgcagc agtgagctaa cacaacgcct gaaatgatgc ataaagcagc gactggattg
    18901 agattttcct gaattagtga gctgatccgc agcaatattt tgtttatcct gtattttcag
    18961 agggaatgga gtgtaacgct ctgtattaac aaggagagca ttaaaatggg taaactcacg
    19021 ggcaagacag cactgattac gggcgcattg cagggaattg gcgaaggaat tgccagaact
    19081 tttgcgcgtc atggcgcaaa cctaatcttg ctggatatct cccctgagat cgaaaagctg
    19141 gcggacgaac tgtgtggtcg tggtcatcgc tgtacggcgg ttgtcgccga tgtgcgtgac
    19201 ccggcgtcgg tagccgcagc tatcaaacgc gcgaaggaaa aagaagggcg cattgatatc
    19261 ctggtgaata acgcaggcgt ttgtcgtctg ggcagtttcc tcgatatgag cgatgacgat
    19321 cgcgatttcc atattgacat caatattaaa ggcgtatgga acgtcacgaa ggcggtgctg
    19381 ccggagatga ttgcccgcaa agatggtcgc attgtgatga tgtcttcagt cactggtgat
    19441 atggtggccg atcctggcga aacggcgtac gccttaacga aagcggcgat tgttggcctg
    19501 acaaaatcgc tggcggtgga gtacgcgcag tctggtattc gcgttaacgc catttgcccg
    19561 ggatacgtgc gcacaccaat ggcggaaagc attgcccgcc agtcgaaccc ggaagatcca
    19621 gagtcggtgc tgactgaaat ggcgaaagca atcccgatgc gtcgcctcgc cgatccgctg
    19681 gaagtcggcg aactggcggc cttcctcgca tcggatgaat ccagctattt aaccggtaca
    19741 cagaatgtga ttgatggcgg cagcacactg ccggagacgg ttagcgtcgg tatctgattc
    19801 acctctgttt cctccctgca tttgtgggga ggatttcgtc ttattccctt ttcaacttcc
    19861 aaatcaccaa acggtatata aaaccgttac tcctttcacg tccgttataa atatgatggc
    19921 tattagaaag tcattaaatt tataagggtg cgcaatggcc gttaacttac tgaaaaagaa
    19981 ctcactcgcg ctggtcgctt ctctgctgct ggcgggccat gtacaggcaa cggaactgct
    20041 gaacagttct tatgacgtct cccgcgagct gtttgccgcc ctgaacccgc cgtttgagca
    20101 acaatgggcc aaagataacg gcggtgacaa actgacgata aaacaatctc atgccgggtc
    20161 atcaaaacag gcgctggcaa ttttgcaggg cttaaaagca gacgttgtca cttataacca
    20221 ggtgaccgac gtacaaatcc tgcatgacaa aggcaagctg atcccggccg actggcagtc
    20281 gcgcctgccg aataacagct cgccgttcta ctccaccatg ggcttcctgg tgcgtaaggg
    20341 caacccgaag aatatccacg actggaacga cctggtgcgc tccgacgtga agctgatttt
    20401 cccaaacccg aaaacgtcgg gtaacgcgcg ttatacctat ctggcggcat ggggcgcagc
    20461 ggacaaagct gacggtggcg acaaagccaa aaccgaacag tttatgactc agttcctgaa
    20521 aaacgttgaa gtgttcgata ccggcggtcg tggcgcgacc accaccttcg ccgagcgcgg
    20581 cctgggcgat gtgctgatca gcttcgagtc ggaagtgaac aacatccgca aacagtatga
    20641 agcgcagggc tttgaagtgg tgattccgaa aaccaacatt ctggcggaat tcccggtggc
    20701 gtgggtcgat aaaaacgtgc aggccaacgg tacggaaaaa gccgccaaag cctacctgaa
    20761 ctggctctac agcccgcagg cgcaaaccat catcaccgac tattactacc gcgtgaataa
    20821 cccggaagtc atggacaaac tgaaagataa attcccgcag accgagctgt tccgcgtgga
    20881 agacaaattt ggctcctggc cggaagtgat gaaaacccac ttcaccagcg gcggcgagtt
    20941 agacaagctg ttagcggcgg ggcgtaatta atgtttgcgg tttcttcaag acgcgtgctg
    21001 ccgggcttta ccttaagcct cggcaccagt ctgctgtttg tttgcctgat tttgctgctg
    21061 ccactctccg cgctggtgat gcagctggcc cagatgagct gggcgcagta ctgggaggtg
    21121 atcaccaacc cgcaggtggt cgcagcctac aaagtgacgc tgctgtcggc gtttgtggca
    21181 tcgattttta acggcgtttt cggtctgctg atggcgtgga tcttaactcg ttatcgcttc
    21241 cctggccgca cgctgcttga tgcgctgatg gatttaccct ttgcgctgcc aacggcagtc
    21301 gccggtttaa cgctggcctc gctcttttct gtaaacggtt tttacggtga atggctggcg
    21361 aagtttgata tcaaagtcac ctatacctgg ctggggattg cggtggcgat ggcctttacc
    21421 agcattccgt ttgtggtacg taccgtgcag ccggtgctgg aagagttagg cccggaatat
    21481 gaagaagcgg cggaaacact tggtgcaacg cgctggcaga gtttctgcaa agtggtgctg
    21541 ccggagcttt ctccggcgct ggtggcgggc gtggcgctgt cgtttacccg tagtcttggt
    21601 gaatttggcg cggtgatttt tatcgccgga aatatcgcgt ggaagacgga agtgacgtcg
    21661 ctgatgattt ttgtgcgctt gcaggagttt gattacccgg cggcgagcgc gattgcttcg
    21721 gtgatcctcg cggcatccct gatactgctg ttctcaatta acactctgca aagtcgcttt
    21781 ggtcggcgtg tggtaggtca ttaatggcgg aagtgacaca attgaagcgt tatgacgcgc
    21841 gcccgattaa ctggggcaaa tggtttctaa ttggcatcgg gatgctggtt tcggcgttca
    21901 tcctgctggt gccgatgatt tacatcttcg tgcaggcatt cagcaagggg ctgatgccgg
    21961 ttttacagaa tctggccgat ccggacatgt tgcacgcgat ttggctgacg gtgatgattg
    22021 cgctgattgc cgtaccggtg aacctggtgt tcggcattct gctggcctgg ctggtgacgc
    22081 gctttaactt ccccggacgc cagttactgc tgacgctact ggacattcct tttgccgtat
    22141 cgccagtggt tgccggtctg gtgtatctgc tgttctacgg ctccaacggc ccgctcggcg
    22201 gctggctgga tgaacataat ctgcaaatta tgttctcttg gcccggaatg gtgctggtca
    22261 cgatcttcgt gacgtgtccg tttgtggtgc gcgagctggt gccggtgatg ttaagccagg
    22321 gcagccagga agacgaagcg gcaattttgc ttggcgcgtc tggctggcag atgtttcgcc
    22381 gcgtcacatt gccgaacatt cgctgggcgc tgctttatgg cgtggtgctg accaacgccc
    22441 gcgcgattgg cgagtttggc gcagtgtcgg tggtttccgg ctcgattcgc ggcgaaaccc
    22501 tgtcgctgcc gttacagatt gaattgctgg agcaggacta caacaccgtc ggctccttta
    22561 ccgctgcggc gctgttaacg ctgatggcga ttatcaccct gtttttaaaa agtatgttgc
    22621 agtggcgcct ggagaatcag gaaaaacgcg cacagcagga ggaacatcat gagcattgag
    22681 attgccaata ttaagaagtc gtttggtcgc acccaggtgc tgaacgatat ctcactggat
    22741 attccttcag gtcagatggt cgcgttgctg gggccgtccg gttccgggaa aaccacgctg
    22801 ctgcgcatta tcgccgggct ggagcatcaa accagcgggc atattcgctt ccacggcacc
    22861 gacgtgagcc gcctgcacgc acgtgatcgt aaagtcggtt tcgtgttcca gcattacgcg
    22921 ctgttccgcc atatgacggt gttcgacaat atcgcttttg gcctgacggt gctgccgcgt
    22981 cgcgagcgcc cgaatgccgc agccatcaaa gcgaaagtga caaaattgct ggaaatggtc
    23041 cagcttgccc atctggcgga tcgttatccg gcgcagcttt ccggcggcca gaaacagcgc
    23101 gtggcgctgg cgcgcgcgct ggctgtggaa ccgcaaattc tgctgcttga tgaaccgttt
    23161 ggcgcgctgg atgcgcaggt gcgtaaagag ctgcgtcgct ggctgcgtca actccatgaa
    23221 gaactaaaat tcaccagcgt ttttgtgacc cacgatcagg aagaagcgac cgaagtagct
    23281 gatcgtgtag ttgtgatgag ccagggcaat attgaacagg ctgacgcgcc ggatcaggta
    23341 tggcgcgaac cggcgacccg ttttgtgctc gaatttatgg gcgaagtgaa ccgcctgcag
    23401 ggaaccattc gcggcgggca gttccatgtt ggcgcgcatc gctggccgct gggctacaca
    23461 cctgcgtatc aggggccggt ggatctcttc ctgcgccctt gggaagtgga tatcagccgc
    23521 cgtaccagcc tcgattcgcc gctgccggta caggtactgg aagccagccc gaaaggtcac
    23581 tacacccaat tagtggtgca gccgctgggg tggtacaacg aaccgctgac ggtcgtgatg
    23641 catggcgacg atgccccgca gcgtggcgag cgtttattcg ttggtctgca acatgcgcgg
    23701 ctgtataacg gcgacgagcg tatcgaaacc cgcgatgagg aacttgctct cgcacaaagc
    23761 gcctgatagg ttgagtgaat gttaaacgcc cggaggcgct tcccgcgatc cgggcttttt
    23821 aatggcaagg tttgtaacct gtaggcctga taagacgcgc aagcgtcgca tcaggcaaca
    23881 ccacgtatgg atagagatcg tgagtacatt agaacaaaca ataggcaata cgcctctggt
    23941 gaagttgcag cgaatggggc cgaataacgg cagtgaagtg tggttaaaac tggaaggcaa
    24001 taacccggca ggttcggtaa aagatcgtgc ggcgctttcg atgatcgtcg aggcggaaaa
    24061 gcgcggggaa attaaaccgg gtgatgtgtt aatcgaagcc accagtggta acaccggcat
    24121 tgcgctggca atgattgccg cgcttaaagg ctatcgcatg aaattgctga tgcccgacaa
    24181 catgagccag gaacgccgtg cggcgatgcg tgcttatggt gcggaactga ttcttgtcac
    24241 caaagagcag ggcatggaag gtgcgcgcga tctggcgctg gagatggcta atcgtggtga
    24301 aggaaagctg ctcgatcagt tcaataatcc cgataaccct tacgcccatt acaccactac
    24361 cgggccggaa atctggcagc aaactggcgg gcgcattacc cattttgtct ccagcatggg
    24421 aacaaccggc actatcaccg gcgtttcacg ctttatgcgc gaacaatcca aaccggtgac
    24481 cattgttggc ctgcagccgg aagagggcag tagcattccc ggcattcgcc gctggcctgc
    24541 ggaatatctg ccggggattt tcaacgcttc tctggtggat gaggtgctgg atattcatca
    24601 gcgcgatgcg gaaaacacca tgcgcgaact ggcggtgcgg gaaggaatat tctgtggcgt
    24661 cagctccggc ggcgcggttg ccggagcact gcgggtggca aaagctaacc ctggtgcggt
    24721 ggtggtggcg atcatctgcg atcgtggcga tcgctacctt tccaccgggg tgtttgggga
    24781 agagcacttt agccaggggg cggggattta aggattaata gcatcggaga ctgatgacaa
    24841 acgcaaaact gcctgatgcg ctacgcttat caggcctaca aggtttctac aatatattga
    24901 attagcacga ttttgtaggc cggataaggc gtttacgccg catccggcat aaacaaagcg
    24961 cactttttta acagttgttg ctgccgacaa atgcagtatt taattttcgt gaggaaacgc
    25021 cgtaaggtca tcaatcattt tttgaagtat tggtgagtcc tgaccgtcac cccattgaag
    25081 aatttttgcg gtaagctgat gacgcgctag tgcattgagt cccatctttt taagtttatg
    25141 ggagatttta cccatgactt taatctgacc gaatgcagtt gcgtaggtaa tcatattgct
    25201 ctggatagtg ctcatttcat cgactagtaa ttgcgcacga acatcctctt caagaatact
    25261 gtccatagcg atgtatttca taccccacat agtttcaatt aacatttgag ggaaatcagc
    25321 acagtccaga tccggctctt tacggaggct attttcaaga acgtttagag catcagcacc
    25381 ttcatcgctg ccaaaaggag attcttcatc ccagcaacac atataaagtg gatcagtaaa
    25441 atggcactgg aagtttgggt gtgacgtttt aacatgtaac ccaatttctt catcatcaaa
    25501 atagtagcga tccatgaagt taaactttgg atcttcttga tagcctttct tcatttttgc
    25561 ggcaatcaat ttactggctt ctttcagaca ctgttcttca ttatcgaact cttttgtctg
    25621 gaatttacca atactaccta ccttgccata gttgacagct aaactatccc cttcgtaatc
    25681 tatccaccaa aatttattcg atttttcatc gtgataaata aagcgttttt tcatgttacc
    25741 tcctgtaaat gccttcccaa agcggattaa tttccttccg caggcaccgt tttatccgtc
    25801 tgcgcaatta acgcatgtaa aaaaggctgt gcgttctcat cgctcttacc ggggatcttc
    25861 acaatatacg gcttcccagt aatcgatgac gacgaagcaa ccttatcaat aaactgctcg
    25921 gcagtgtcaa tgcggttacg ggtattgccg agcttcagac gcagatgaga aaccgcttca
    25981 tagcaggtat gttcatcacc gttgcgcaca aatatcaaat cctttttctg tgctaatccc
    26041 tccagcatgg cgttgatgcg ggcttcttca tgggcagtta acttcgcgta aacggggagt
    26101 gtcattagta gtgtaatgac cagacaaatg attttcttca ttgaattacc ttatccttgt
    26161 tttgtgatag ccgatttgac tgccagaatc aggtaaagtt tcggttggat tacgatgagg
    26221 atcatcggca aggagaaaaa attaaatgag tagtttgttg ttgtttaacg ataagagtag
    26281 ggcactgcag gcggatatcg tcgccgtgca gtcgcaggtg gtttacggca gcgtgggcaa
    26341 cagcattgcc gtgcctgcta tcaaacagaa cggcctgaat gtctttgccg tgccgacggt
    26401 attgctgagc aatacgccgc attatgacac tttctacggt ggtgcgattc cggacgaatg
    26461 gtttagcggc tatttgcgtg cgcttcagga gcgtgatgcg ctgcgccaac ttcgtgctgt
    26521 aaccacgggc tatatgggaa cggcatcgca aatcaaaatc cttgccgagt ggctgactgc
    26581 gctacgcaaa gaccatcctg acctattgat catggtcgat ccggtgattg gcgatattga
    26641 tagcggaatt tatgtcaaac ctgaccttcc cgaagcgtat cgacaatatt tactgccgct
    26701 ggcgcaggga attaccccca atatctttga gttggaaatc ctgaccggta aaaattgccg
    26761 cgatctcgac agtgccattg ctgccgcaaa aagtctgctt tcagacacat taaaatgggt
    26821 ggtggttacc agcgcctccg gtaatgaaga aaatcaggag atgcaggttg tggtggtcac
    26881 tgccgacagc gtgaatgtca tttcccattc acgggtaaaa accgacctga aagggactgg
    26941 cgacctgttt tgtgctcagc tcatcagtgg cttgctgaaa gggaaggcgt taaccgatgc
    27001 agtgcaccga gcggggttgc gcgtactgga agtgatgcgc tacacccagc agcatgagag
    27061 cgatgaattg attttgccgc cgctggcgga agcataaaaa aatggcgccg atgggcgcca
    27121 tttttcactg cggcaagaat tacttcttga tgcggataac cggggtttca cccacggtta
    27181 cgctaccgga cagtttgatc agttctttga tttcgtccat gttggagata acaaccggag
    27241 tcagggtaga cttggctttc tcttccagca gcggcagatc aaattcaatg acagtatcgc
    27301 caactttcac gcgctgacct tcttcagcaa tacgcttgaa gccttcgcct ttcagttcaa
    27361 cggtgtcgat accgaagtgg acgaacagtt caacgccgct atcagattcg atagagaatg
    27421 cgtggttggt ttcaaagatt ttaccaatgg tgccgtctac tggcgcgacc attttattac
    27481 ccgttggttt gatagcaata ccatcaccaa cgattttttc cgcaaaaacg acatccggca
    27541 cgtcttcgat attgacgatc tcgccagaga gcggagcaat gatctcaata gttccggtat
    27601 ccttcttgtc gtcggaaacc agagatttca gtttatcgaa caaacccatg atcttctcct
    27661 aagcagtaaa ttgggccgca tctcgtggat tagcagattg ttttttcttc aatgaacttg
    27721 ttaaccagcg tcattaactc gtccgttgtc ggttgagcaa gagcctgctc tgctaacacc
    27781 ttcgcatctt cgaagttcgt gttacggata atcttcttaa tgcgcgggat agaaatggcg
    27841 ctcatagaga attcgtccag acccatcccc agcaacagaa gtgtagcacg ttcatcgcca
    27901 gcaagctcac cacacatgcc agtccatttg ccttcagcat gagaagcatc aataacttgc
    27961 ttgatcaagt tcagcacgga cggtgacatt ggctggtaaa ggtgtgaaat catatcatta
    28021 ccacggtcaa ctgccagagt gtactgcgtt aaatcattgg tgccgatact aaagaaatca
    28081 acttctttgg ctaaatgacg tgcaattgtt gcggcagccg gtgtttccac cattacgccg
    28141 atttcaattg actcgtcaaa cgctttacct tcgtcgcgca gttcctgttt gtagatttcg
    28201 atctctttgc gcagtgcacg cacttcttca acagagatga tcatcgggaa cataatgcgc
    28261 aatttaccga aagccgaggc acgcaggata gcgcggagct gatcgcgcag gatctcttta
    28321 cgatccattg cgatacggat agcgcgccag ccgaggaacg ggttctcttc tttcgggaag
    28381 ttcatgtatg gcagctcttt gtcgccgccg atgtccatgg tacgaacgat aaccgcctga
    28441 gagccacacg cttcagccac tgctttgtaa gcagcaaact gttcttcttc agtgggcagc
    28501 gcgtcgcggt ccatgaacag gaactcagta cgatacagac caacgccttc agcgccgtta
    28561 cgctctgcac cttcaacgtc acgaaccgta ccaatgttag cgcatacttc tacctggtga
    28621 ccgtccagcg taatagctgg cagatctttc agtttagcaa gctctgcttt ttcagaagcc
    28681 acttgctcct gaacagcgcg cattttatca ataacttcgt tggttggatt gacgtaaacc
    28741 tgattattta cggcatccag aatcagatag tcgtcatttt tcacctgaga ggtgacgcta
    28801 ccggtaccca cgatagcagg cagttccaga gaacgcgcca tgatagaggt gtgggaagta
    28861 cggccacccg cgtcggtgat gaaacccagc accttcttca ggttcagctg tgcggtttcg
    28921 gacggcgtca ggtcagcggc aaccagaatg acttcatcct gaatggcgct caggtcgata
    28981 atcttcaggc ccaggatgtt gcgcagcagg cgcttaccga tatcacgtac gtcagccgca
    29041 cgttctttca ggtattcatc atccagctct tccagggcag aagcctgacc ttcgataact
    29101 tcatgagcag ctgcgtcagc tgtcatgtgc ttatctttaa tcagggctat gatttcctgc
    29161 tccagctcct catcttcgag cagcataata tgcccttcaa agatggcttc tttttcttca
    29221 ccgaacgttt caccagcttt cgttttgatc gtttccagct gggctgatgc cttggcacga
    29281 ccgctcagaa aacgttcaac ttcctgatca acctggtcgg cagaaatttt tttccggtca
    29341 atgacaattt cgtcttcttt cagaagcaga gctttaccga aagcgatacc cggggatgct
    29401 aaaatgcctg aaatcataac cctaccttac ttgtgactga tttttaaaag aacccgggaa
    29461 attactcgag ttccgccatc agtttaacca gatgttcaac cgctttctgc tcgtcttcgc
    29521 cttctgcgga gatagtcaca acggtacctt gagtcaggcc cagagtctgc agtttaaaca
    29581 ggcttttcgc gctggcgctt ttgccgttgg aagtcacagt aatttcagaa gtgaagccct
    29641 tagcttcttt tacaaactgg gcagcagggc gggtgtgcag accgttcgga gcggtaatgg
    29701 taacttcttg ctggaacatt gtatttcccc aacttatagg tttagtgttg tggaactaaa
    29761 gtctagcctg gcggttcgac tttagcctgt attgttagcg ccagttttta acagacgcga
    29821 cgcacgaaga gcgcaataca gtccattgct ggcgggtgtt gaccgctgac gtttcgtacg
    29881 tcattaaaca ttatgccgct aaaggaagaa ttgaaccaaa tcataaaatc gattcagctt
    29941 tggcttttcc tgtaacgatt aatttcgcgc atcaaaataa ttagtctggt taaataccag
    30001 ttccgacagg ctgaatcaat gccaggaggg gcgaaagttt gaagcaggcc acaaaaaagc
    30061 acctaaaaag gtgctttttt acgcattttt aacaagctgg cattactgtt gcaattcttt
    30121 ctcagtgaag agatcggcaa acaatgcggt gcttaaataa cgctcacccg atgatggtag
    30181 aataaccaca atattcttgt tggtaaagct ttcatcttct tgtagtttca acgccgcggc
    30241 aacagctgct ccagaagaga tacctgcaag aataccttct tcttccatca gacgacgcgc
    30301 ggtagaaatc gcttcttcat tggtgatgcc aatgacttta tcgaccagct tgagatcgag
    30361 gttagccggg ataaaaccag cgccaatacc ctgaatttta tgcgggccag gtttaatctc
    30421 ttcacctgcc agcgcctggg cgataactgg agaatcggtt ggctcaacgg cgacagagat
    30481 aagatcggtc ttgcctttgg tgcctttaat gtagcggctg acgccagtca gcgtaccgcc
    30541 agtcccaacg ccagcaataa atacatcaac ctgaccgtcg gtatcttccc atatctccgg
    30601 accggtggtc ttttcgtgaa tttcagggtt tgccggattg ctgaattgtt gcagcagcag
    30661 gtatttctct ggattgctgg cgacaatttc ttctgctttt tggattgcgc ctttcatgcc
    30721 tttagcacct tccgtcagca ccaggtttgc acctaacgct ttcagcagct tgcggcgttc
    30781 aatactcatg gtttctggca tggtcagggt gagtttgtaa ccgcgagcgg cagctacata
    30841 ggccagtgca atcccggtat taccgctggt cggttcaacc agttcaacgc ctggtttcag
    30901 cacgccgcgc ttttcggcat cccaaatcat gttggcaccg atacggcact taacgctgaa
    30961 gctggggtta cgagattcca ccttcgccag aatgcgtccg ttaccgatgc gattcaggcg
    31021 aaccagcggc gtgtgaccga tagtcagcga gttatcttca aaaatcttac tcatggcctg
    31081 tccttaactg tatgaaattg ggatacaaca ggtagcatac ccgctcagag aatatgcgga
    31141 agtaaggatt tagcatatct atatacagaa gggaaataat gacatgcaag atggaataag
    31201 gggcggcata agccacccct gtttcacaca aacggtttac aaaataaccg gtagattgtt
    31261 accgccacat cgcgtgttta tcgcgatagc aatcgaccca catcgccgtc gcgccacaaa
    31321 cggcaacggg catgatgaac agattaagca gcgggatcat cgtaaacagg ctggttaaag
    31381 caccaaactg catattggtg attttgcgtg tgcgcagggc ggtgcgcatc tctttaaacg
    31441 gcactttgtg gttatcgaag gggtaatcgc aatactggat ggctaacatc caggcgctaa
    31501 acaggaacca cagtaccggc gcgacggttt gcccaatacc agggatgaag taaagaatta
    31561 gcaatacaat tgcgcgtggc agataccagg caaatttttg ccattcgcgt ttcatgattc
    31621 gcggcacatc tttcatgata ccgaaaatcc cggtatctgg cggtgtagcg ccagtcaatc
    31681 gtgcttccag ttgttcagcc aataaaccgt tgaacggagc ggcaatccag ttagcaatcg
    31741 ttgagaagaa atagccaaac actaacagca cagagatgac agccaggggc cacaacagat
    31801 agctcagcca ttgtaaccag tccggaacat aactcatgag agtcgggatc cagacatcga
    31861 gttgcgtaaa gagccaccag aatgcgcccc ccatcaacaa aatattgacc agcagcggta
    31921 aaataacgaa acgccgaatc ccaggttgcg agacgagctt ccagccttgc gcaaaatagt
    31981 aaaaaccgct gcgtggggca gatgtgaatg atgaaaccat aatcaggatg agctcctttt
    32041 gaccaatccc aggaaaattc tgcgtatttt accgggtaat tgcgcaatgg acagttagga
    32101 tatgttcgaa aaaacagcaa aaagcacgat ttcatctatc tttgtgctgt gaaagttaat
    32161 agtgcacttg cacttgaggt aatcggcaaa tactcttagt gagtaaatgt ttgccgtggt
    32221 ggcaaggtgt tagaacaaca gagaatataa tgatgcagga tttgcgtctg atattaatca
    32281 ttgttggcgc gatcgccata atcgctttac tggtacatgg tttctggacc agccgtaaag
    32341 aacgatcttc tatgttccgc gatcggccat taaaacgaat gaagtcaaaa cgtgacgacg
    32401 attcttatga cgaggatgtc gaagatgatg agggcgttgg tgaggttcgt gttcaccgcg
    32461 tgaatcatgc cccggctaac gctcaggagc atgaggctgc tcgtccgtcg ccgcaacacc
    32521 agtaccaacc gccttatgcg tctgcgcagc cgcgtcaacc ggtccagcag ccgtctgaag
    32581 cgcaggtacc gccgcaacat gctccgcatc cagcgcagcc ggtgcagcag cctgcctatc
    32641 agccgcagcc tgaacagccg ttgcagcagc cagtttcgcc acaggtcgcg ccagcgccgc
    32701 agcctgtgca ttcagcaccg caaccggcac aacaggcttt ccagcctgca gaacccgtag
    32761 cggcaccaca gcctgagcct gtagcggaac ctgctccagt tatggataaa ccgaagcgca
    32821 aagaagcggt gattatcatg aacgtcgcgg cgcatcacgg tagcgagcta aacggtgaac
    32881 tgcttcttaa cagcattcaa caagcgggct tcatttttgg cgatatgaat atttaccatc
    32941 gtcatcttag cccggatggc agcggcccgg cgttattcag cctggcgaat atggtgaaac
    33001 cgggaacctt tgatcctgaa atgaaggatt tcactactcc gggtgtcact atctttatgc
    33061 aggtaccgtc ttacggtgac gagctgcaga acttcaagct gatgctgcaa tctgcgcagc
    33121 atattgccga tgaagtgggc ggtgtcgtgc ttgacgatca gcgccgtatg atgactccgc
    33181 agaaattgcg cgagtaccag gacatcatcc gcgaagtcaa agacgccaac gcctgataca
    33241 cttaaggcaa attaactcct cttcgaaccc ccgcttgtcg ggggttttta gcattgatgg
    33301 tgcgatatgg aatcaatcga acaacaactg acagaactgc gaacgacgct tcgccatcat
    33361 gaatatcttt atcatgtgat ggatgcgccg gaaattcccg acgctgaata cgacaggctg
    33421 atgcgcgaac tgcgcgagct ggaaaccaaa catccagaac tgattacgcc tgattcgcct
    33481 actcaacgtg taggcgctgc gccgctggcg gctttcagcc agatacgcca tgaagtacca
    33541 atgctgtcac tggataacgt ttttgatgaa gaaagctttc ttgctttcaa caaacgtgtg
    33601 caggaccgtc tgaaaaacaa cgagaaagtc acctggtgct gtgagctgaa gctggatggc
    33661 cttgccgtca gtattctgta tgaaaatggc gttttagtca gtgccgcgac ccgtggcgat
    33721 ggcaccaccg gggaagatat cacgtctaat gtgcgtacta ttcgcgccat tccgctgaag
    33781 ctgcacggag agaatatccc ggcgcgtctg gaagtgcgtg gtgaagtgtt cctgccgcag
    33841 gcggggttcg aaaagattaa cgaagatgcg cgacgcacgg gcgggaaagt ttttgctaac
    33901 ccacgtaatg cggcagctgg ttcactgcgt cagcttgatc cgcgtattac agcgaagcga
    33961 ccgctcactt ttttctgcta tggcgttggt gttctggaag gtggcgagct gccggatact
    34021 catcttggcc gtttactgca atttaaaaag tgggggttgc cggtcagcga tcgggtaacg
    34081 ctttgtgaat cggcggaaga agtgctggcg ttctatcaca aagtggaaga agaccgcccg
    34141 acgctgggct ttgatatcga cggcgtggtg attaaggtca actcactgga acagcaggag
    34201 cagcttggct ttgtcgcgcg tgccccgcgc tgggcggtag cgtttaaatt cccggcgcag
    34261 gagcagatga cctttgtgcg tgacgtcgag tttcaggttg ggcgtactgg cgcgattacg
    34321 cctgttgcgc gtctggaacc tgtccatgtt gctggcgtgc tggtgagtaa cgcaacctta
    34381 cacaatgcgg atgaaatcga acgtcttggt ttacgcattg gcgataaagt ggtgattcgc
    34441 cgcgctggcg acgtgatccc gcaggtggtt aacgtcgtgc tttctgaacg cccggaagat
    34501 acccgtgagg ttgtattccc gacgcattgt ccggtatgtg gttctgacgt tgagcgtgtg
    34561 gaaggtgaag cggttgcccg ctgtaccggt ggcctgattt gcggtgcgca gcgtaaagag
    34621 tcgctgaaac actttgtttc ccgccgtgcg atggatgttg acggaatggg cgacaaaatc
    34681 atcgatcagc tggttgaaaa agaatatgtc catactccgg cagatctgtt caaactcacc
    34741 gcaggcaaac tgaccggact ggagcgtatg gggccaaaat cggcacaaaa cgtggttaac
    34801 gcgctggaaa aagcgaaaga aaccaccttt gctcgcttcc tctatgcact tggcatccgt
    34861 gaagtcggcg aggccaccgc agcaggtctg gcggcatatt tcggcacgct ggaagcgctg
    34921 gaagccgctt cgattgaaga gctgcaaaag gtgcctgatg ttggcattgt cgttgcatcc
    34981 cacgttcaca acttctttgc cgaagaaagc aaccgcaatg tcatcagcga gctgttggcg
    35041 gaaggtgttc actggcctgc gccgatcgtt atcaacgcgg aagagattga cagcccgttt
    35101 gctggtaaaa ccgtggtgct tacgggcagc ttaagccaga tgtcgcgtga tgacgctaaa
    35161 gctcgactgg ttgaactggg cgcgaaagtc gcgggcagcg tgtcgaagaa aaccgatctg
    35221 gtgattgccg gagaagcggc aggttctaaa ctggcgaaag cgcaggaact gggcattgaa
    35281 gtcatcgacg aagcggaaat gctgcgtttg ctgggtagct gagatggaaa aagagcagct
    35341 gattgaaata gccaatacga taatgccgtt tggcaaatat aaagggcgtc gcttaatcga
    35401 cctgccggag gagtatctgc tgtggtttgc ccgcaaagat gaattcccgg cagggaagct
    35461 cggtgagcta atgcaaatca cgctgctgat taaaaccgag gggctgacgc aactggtcca
    35521 gcccctgaag cgtccgcttt aagctttatc ggcgctgctt tcctgctgtg cctgtaactg
    35581 ttcggtctgg cgtttgtatc gacgcgccag caccgcacag accatcaatt ggatctgatg
    35641 gaaaatcatc aggggcagca ccatcatacc gatcaccgat gtggggaaca gaatgtttgc
    35701 catcgggatg ccatttgcca gactcttttt cgaaccacaa aagacgatag taatttcatc
    35761 tgccttattg aagcccagtc ggcgtgccat aaagacgtta actacaatca cgatagccag
    35821 aagaacgcag ctgaccacca cgataaacag caatgatccc cagccaactt tatgccagat
    35881 accattaacg acggcttcgc tgaacgctgt ataaaccacc aacagaatgg acgtctggtc
    35941 agttttcgca atccattttt tattgcgcga cacccagtca ccaatccacg gccgggaaag
    36001 atgccccaac acaaacggca gcagcagttg cagcataatt ttaccgacct gctcaaggct
    36061 gccccctgca ccgtgaacat tcatcaccag accaaccagc aatggtgaaa ggaaaatccc
    36121 cagcaggctg gatgctgacg cagaacaaac cgccgccgcg acgttaccgc ccgccattga
    36181 cgtgaaggcg attgcagact gcacggtagc cgggagaatg cacaagtaga gaaaaccgga
    36241 gtagagcatc gggtcgacat ttaccggttt ccaccaggca aacagtacac ccagaatcgg
    36301 aaacagcacg aaggtgctgc acattaccca caaatgcagt cgccagtgac caccgccagc
    36361 aataatcgcc tcacgcgaca acttcgcgcc gtgcataaag aacagcaggg caatagctgc
    36421 ggtggtcaga ttttcaaaga aggggacgaa atcgcctctg gccggaaaga aagaggccag
    36481 caacaccacc gtgatcaggg ttaaggtgaa aggatcgagg atacgaaaaa gtttcataaa
    36541 gactccagat gatcgatggt tctattgtgc ttcttccagt ttgagaaata aaattgattt
    36601 attgcatcca ttcatgaaga aaacagatga attattcttt aaaacaatta aaggttttcg
    36661 tcacagtagc gcaggagaaa agttttagtc gtgcaggaga gcgtatcggc ctgagccagt
    36721 cggcagtgag tcacagtgtg aaggagctgg aaaatcatac tggtgttcgc ctgctggaca
    36781 gaaccacgcg tgaagtggtg cttacagatg cagggcagca gctgtctttg cgtcttgagc
    36841 gactgctgga tgaactgaac agcacgttgc gcgataccgg gcgtatgggg caacaactga
    36901 gcggaaaagt tcgggtcgct gccagccaga ccatttccgc gcatcttatt ccgcaatgca
    36961 ttgctgaaag ccatcgccgc tatccagata ttcagtttgt cctgcacgat cgtccgcagc
    37021 agtgggtgat ggaaagtatt cgccagggag atgtcgattt tggcatcgtc atcgatccag
    37081 gcccagtggg cgatctgcaa tgtgaagcga ttctttccga gcctttcttt ctgctttgcc
    37141 atcgcgatag cgctttggcc gtggaagatt atgtaccctg gcaggcatta cagggagcga
    37201 aattggtgtt gcaggattac gcgtcaggca gccgaccgct gattgacgca gcgctggcgc
    37261 gcaatggtat tcaggcgaat attgtgcagg agattggaca tccggcgacg ctgttcccga
    37321 tggtagccgc aggcatcggc atcagtattc ttcctgcact ggcgctacca ttgcctgaag
    37381 gtagcccact ggtggtaaag cgcattacgc cggttgttga acgccagttg atgctggtgc
    37441 gcaggaagaa ccgttcactt tccaccgccg ctgaagcttt atgggacgtg gtacgagacc
    37501 agggcaatgc gctaatggct gggcgggaag gggatccgtt gtatcagata tagagtgaat
    37561 tcatactgcc gggagccccg gcagtaatca atcagaagtt aaatttgatg gagtagatta
    37621 tcgcatcctg gtaaaaatct tcgcccagtt tgtcatccgc atatcgatac tgaatccccg
    37681 tagtaatgtg tgatgttgca ttccaccaca gtgcaactgc gccattcagc ccttctttac
    37741 cgccattacc cgctgcatag gtagcgtcac ggtcaaactc gtactcgttc cagttggtaa
    37801 gagtgaattt ctcgctgccc agcataaagt tgtaacctgc aacccagccg gcgacatagc
    37861 cgttgtcgcc agtgtaatag gtttgatctg tataacgctt agcaaagaac ggtttgaacc
    37921 accaaccgct gtcggtgaaa ttgtagccga taccgtacag gaacatatcg tcgtggaaat
    37981 tcacgcgatt cgcagaaccg taggtgccat acgcatgcaa gtagagatta aatccggtgt
    38041 cacccaggta aatacggttg gtatttttaa aggtataacg ctgctcgcta cctggtttat
    38101 tatggcgacc gttatagaag ttttcccagt cgaagaaccc gtacatttct ccccaactaa
    38161 agttagcgcc accttcaaat tcaagatagc caaaatcatc tttgtgtgat ttggtcgacg
    38221 atttttcggt agtctggctg gttcagtcca gataatgcac gccgatatct gcaaaaccgc
    38281 ctttaaattc tgcatgagat acaaaaggta tcgctaatat agaggaaagt gtcagagtta
    38341 aaagatgttt tttcatagca taattcccta tgccgatcgt tattagtagg ttgcttaata
    38401 aagtggtttg ctaaaaacgg catattgtta atgcaaagga attaatatcg ccaatacagt
    38461 ttttaccgtg gattaataga gaaaagatat aaatccatcg atatcgaatc gctattgaat
    38521 gcgtgagaaa agtgtattgg taagagccgg gacaacctcg ctataaaaag gcgttagatt
    38581 ccaccctaca gaaaaaggat atatatgtct caggttcaat tttctcataa cccgctgttt
    38641 tgcatagata ttatcaagac ttataaacct gatttcacgc cacgagtggc ctttatttta
    38701 ggttccgggc tgggcgcgct ggccgatcag attgagaacg ctgtcgcaat ttcctacgaa
    38761 aagctgccgg gcttcccggt aagtactgta catggtcatg cgggggagtt agtgctgggc
    38821 catctccagg gcgtaccggt ggtatgtatg aaaggtcgcg gacatttcta cgaaggtcgt
    38881 ggaatgacca ttatgactga cgcaattcgt acctttaagc tgctgggctg cgagctactg
    38941 ttctgcacca atgcggcagg ctcgctgcgt ccggaagtgg gggcaggcag cctggtcgca
    39001 ttgaaagatc atatcaacac catgccaggt acgccgatgg tgggacttaa cgatgatcgt
    39061 tttggagagc gtttcttctc actggcaaat gcctacgatg cggaataccg cgcactgtta
    39121 caaaaagtgg cgaaagaaga gggtttccct ctgacggagg gcgtgttcgt ctcgtatccg
    39181 gggccgaatt tcgagactgc ggcggaaatt cgcatgatgc aaattattgg tggggatgtt
    39241 gttggtatgt ctgtggtgcc tgaggttatt tcagctcgcc attgcgacct taaagtcgtt
    39301 gcggtctctg cgattaccaa tatggcggaa ggtttaagcg atgtgaaatt gtcacatgcg
    39361 caaacgctgg cggctgcgga gctctctaag cagaacttca tcaaccttat ttgtggcttt
    39421 ctgcgcaaaa tagcctgaga aaaacaataa gaaacggcct cgcgggtgag gccatatgat
    39481 gcgacaagga aacgattatg agcatcgcta tgcgcttaaa ggtaatgtcc tttttgcaat
    39541 attttatctg ggggagctgg ctggttaccc tcggctctta catgattaat acccttcatt
    39601 tcaccggcgc taatgttggc atggtttaca gttccaaggg gatcgccgcg attattatgc
    39661 ctggtataat ggggatcatc gcagacaaat ggctgcgcgc agaacgtgca tacatgctgt
    39721 gtcacctggt gtgtgcgggc gtactttttt atgcggcatc cgtaactgat ccggatatga
    39781 tgttttgggc gatgttagtc aatgcgatgg cgtttatgcc gactattgcg ttatcgaaca
    39841 gcgtctctta ttcctgtctt gcccaggccg ggcttgaccc ggtgaccgct ttcccaccca
    39901 ttcgcgtttt tggtacggtg gggttcattg tcgcgatgtg ggcagtaagc ctgctgcatc
    39961 tggaattgag tagtctgcag ctgtatatcg cgtccggtgc gtcattgctg ctgtcggctt
    40021 atgcgctgac tttgccgaag attccggttg ctgagaaaaa agcgaccaca tcgcttgcca
    40081 gcaagctggg tctggatgcc ttcgtgctgt ttaaaaatcc acgcatggcc atctttttcc
    40141 tctttgccat gatgctgggt gcggtactgc aaattaccaa cgtttttggt aatccgttcc
    40201 tacatgattt cgcccgtaac ccggagtttg ctgacagttt tgtggtgaaa tacccctcca
    40261 ttttgctgtc ggtatcacag atggcagaag tgggctttat actgactatc ccattctttt
    40321 taaagcgatt tggcattaaa accgtcatgc tgatgagtat ggtggcctgg acgctgcgct
    40381 ttggcttctt cgcctatggc gatccgtcaa caaccggatt tattttgctg ctgctgtcga
    40441 tgattgttta tggctgtgca ttcgatttct tcaatatttc tggttcggta tttgtcgaac
    40501 aggaagttga ttccagcatt cgtgccagcg cgcaggggct ctttatgacc atggtaaatg
    40561 gtgtcggcgc atgggttggc tcgattctga gtggcatggc agtagattac ttttcggtgg
    40621 atggcgtaaa agactggcaa actatctggc tggtgtttgc aggatatgct ctttttctcg
    40681 cagtgatatt tttctttggg tttaaatata atcatgaccc tgaaaagata aagcatcgag
    40741 cggtgactca ttaaaaggat tcgcggctct gctcttcaga gctgctttta tgataaaggt
    40801 taattagtga aagatatttt attttcttcc ggcgttggtt ttggtattgg tgctttgttt
    40861 acaatcgtgc gcttgcctat tcctgtacca aatgtattac cgggtatatt atccattgtg
    40921 tttatgtatg tcggatatct ggtggtgaaa tactttatgc catgataatt taatacgatg
    40981 tatttattat atggagcact taattatgga acgcgtatac agaacagatc ttaagttgct
    41041 ccgttatttt cttgccgtag cggaagagtt gcattttggc cgcgcagcag cgcgtttaaa
    41101 tatgtctcag cctccgctca gcattcatat taaagagctg gaaaatcaac tcggcacgca
    41161 gctttttatt cgccattcgc gcagcgtcgt actgacacac gcgggcaaaa tcttgatgga
    41221 agaatcgcgt cgattgctgg tgaatgctaa taatgtattg gctcggattg aacaaatagg
    41281 tcggggagaa gcagggcgga ttgaactcgg cgtagtggga acggcaatgt ggggacggat
    41341 gcgcccggtt atgcggcgat tcctcaggga aaatcctaac gttgacgttc tttttcgcga
    41401 aaagatgccc gcgatgcaaa tggccttgct ggaacgccgc gaacttgatg ccgggatctg
    41461 gcgaatggcg acagaaccac cgactggttt taccagctta cggttgcatg aatcggcgtt
    41521 tctggtggcg atgcctgaag agcatcatct ctcatcattt tccaccgtcc cgctggaagc
    41581 gctacgtgac gagtattttg ttacaatgcc gcccgtttac actgactggg attttttgca
    41641 gcgagtttgc cagcaggtgg gattttcacc ggttgttatt cgcgaagtta atgaaccgca
    41701 aacggtactc gctatggtca gtatgggcat tggtatcaca ttgatagcgg acagctacgc
    41761 acaaatgaac tggccaggtg tcattttccg tccgctcaaa cagcgcatcc ctgcagattt
    41821 atatattgtt tatgaaacac agcaggtgac gcccgcgatg gttaagctgt tggctgcatt
    41881 gacacagtag gtgaggagac aggaattgcc ggaaatagta gtctgctaag tgaagcgcat
    41941 caggcaattt cgcaaatcgc agatagcaaa aaagcgcctt tagggcgctt ttttacattg
    42001 gtgggtcgtg caggatgact cgcttcgctc gcccttcggg ccgtcgccgc gagcggctcc
    42061 gttgtttcgc tgcgctcaac tcaaacctgc tgcaggttcg aatctttgag tatcgcagat
    42121 agcaaaaaag cgcctttagg gcgctttttt acattggtgg gtcgtgcagg attcgaacct
    42181 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc gaagtggtgg
    42241 gtgatgacgg gatcgaaccg ccgaccccct ccttgtaagg gaggtgctct cccagctgag
    42301 ctaatcaccc acttcggtac ttcacatttt tacaagaaat ttagctggcg agaaagtggt
    42361 gggtgatgac gggatcgaac cgccgacccc ctccttgtaa gggaggtgct ctcccagctg
    42421 agctaatcac ccacttctca atttcttgct acacggcgga gactacataa agtagttggt
    42481 gggtgatgac gggatcgaac cgccgacccc ctccttgtaa gggaggtgct ctcccagctg
    42541 agctaatcac ccccgctgtg tggagtcgca ttatagggag agttcaaaat gagtcaacgc
    42601 attttctaaa gaaattgttc gttcgtcgta aatttaagca agatgatcgc aaaacagacc
    42661 gtgttgcgca atttgtcaac gaaaacaata atgcgtaagg tagaaacccg aactacattg
    42721 aggaatcagg cgggagtgat agaatatcgc ccactcaatt tttccaggat ttgccggttg
    42781 tcggcatctt tctaaacgta aggccatttc atgaaaatca aaactcgctt cgcgccaagc
    42841 ccaacaggct atctgcacgt tggcggcgcg cgtactgctc tttactcctg gctttttgca
    42901 cgtaaccacg gcggtgagtt cgtgctgcgt attgaagaca ccgatcttga gcgttccacg
    42961 ccggaagcta tcgaagccat tatggatggc atgaactggc tgagcctgga gtgggatgaa
    43021 ggtccgtact accagaccaa acgttttgat cgctacaacg cggtgatcga tcagatgctg
    43081 gaagagggca ctgcttataa atgctattgc tctaaagagc gcctggaagc gctgcgcgaa
    43141 gagcaaatgg cgaaaggtga gaagccgcgt tatgacggtc gctgccgcca cagccatgag
    43201 catcatgctg atgatgaacc gtgtgttgta cgttttgcta acccgcagga aggttctgtt
    43261 gtttttgacg atcagatccg tggtccgatc gagttcagca accaggaact ggacgatctt
    43321 attatccgcc gtaccgatgg ttccccaacc tataacttct gtgtggttgt cgatgactgg
    43381 gatatggaaa tcacccacgt tatccgtggc gaagaccata tcaacaacac gccacgccag
    43441 atcaacattc ttaaggccct gaaagcgccg gtgccggttt acgcgcacgt ttctatgatc
    43501 aatggcgatg acggtaaaaa actgtccaaa cgtcacgggg cagtcagcgt aatgcagtat
    43561 cgtgatgacg gttatttgcc agaagcactg ctgaactatc tggtgcgtct gggctggtcc
    43621 cacggcgatc aggaaatctt cactcgtgaa gagatgatca aatacttcac tttgaatgcc
    43681 gtcagcaaat ctgccagtgc gttcaacacc gacaagctgc tgtggctgaa ccatcactac
    43741 attaacgcgc tgccgccgga gtatgttgct actcacttac agtggcacat tgagcaggaa
    43801 aatatcgata cccgtaacgg cccgcagctg gctgatctgg tgaaactgct gggcgaacgc
    43861 tgcaagacgc tgaaagagat ggcacagagc tgccgttatt tctacgaaga ttttgctgag
    43921 ttcgatgccg acgccgcgaa aaaacatctg cgtccggtag cgcgtcagcc gctggaagtg
    43981 gttcgtgaca aactggccgc gattactgac tggaccgctg aaaacgttca tcacgctatt
    44041 caggcgacgg cggatgagct ggaagtgggt atgggtaaag ttggtatgcc gctgcgtgtc
    44101 gccgtaaccg gtgcggggca gtctccagca ctggatgtta ccgttcacgc aattggtaag
    44161 acccgcagta tcgagcgtat caacaaagcg ctggatttta ttgctgaacg cgaaaatcag
    44221 cagtaattag cgtgtaaaaa gataaacggc aggagataat atcctgccgt tttttattta
    44281 tgctgattcg tcaatatcga gacgggcgag gaaattgcga attccttcac gtgacaaaaa
    44341 ttgcgcgact ttttcctgct caatgtctgt catattatct atcgcgttga tgatttgtcg
    44401 atacgccggg gcgcgtgctc caggagcgta ttcagcaatt ctctcttcag cttccatcaa
    44461 cattggtgtg ttatggaagg ctggcgtgtt ctgaataaac tcgcaaactt gcgtatcgac
    44521 cagaataagt ctggcacgac cgcctttcac gccgggaaat ttttccgttt tccagccttt
    44581 ttctctgatc caacggttaa cagtttgttt agccacaccg aggcattcag ccagttcttc
    44641 ggtggtcatt ttattgcgta atcttttcat attatttgct atcgcgaatc cctaagcgtt
    44701 gtaacaatcc gataatccct tcccgcagaa gcaaggatgt aaactgtttt tgttcaactg
    44761 gcgtcatctc tttcgccagt gtcaccagca atacttcaag cggcgcatca ccagaaaggg
    44821 caggcgcttc tccctgacct tctggacgtt cagcattgcg aatatattca cgaacctgtt
    44881 cattgacgtg aaccagtcgg gctttgccac cctggacgcc aggttttggt gacgttgtcc
    44941 agccttcctt gcgtacccat ttattaatgg tctggcggct atagccggtc agtctggcaa
    45001 gttcatctgg tgtcatcctc tccttgaaca taacaattcc ctgaatgtat gtgggtctat
    45061 tagtagttca ttttataaca ccactttaca ggaaaccgtg acatgaataa ctactgaatg
    45121 cgagcggttt tccaaaattg ttcatttgcc tgatttttca gcgattgaaa ctacacgttg
    45181 caatttgccg ttgacacatt cgggcggaat tcatatgatg ccgcccgtca actcgacaag
    45241 ctttacgtga cggggctata gctcagctgg gagagcgctt gcatggcatg caagaggtca
    45301 gcggttcgat cccgcttagc tccaccaaat tttgcaccca gcaaacttgg tacgtaaacg
    45361 catcgtgggg ctatagctca gctgggagag cgcttgcatg gcatgcaaga ggtcagcggt
    45421 tcgatcccgc ttagctccac caaatttcca accctcgctg caaagcgggg gttttttgtc
    45481 tctgcttttt gccgcttttg taatacagtc tacgtccggg ttagtgccgc ctggtgaaag
    45541 catcattgga tgaaaaatcg gcaacaggct ggccccctgt ttgcttcgcg atgcgaataa
    45601 acttattatt tgtgtgcctg aaaaccccga tcagtgagag tagtgtactc atgtttgtgg
    45661 agcataacct gataaaaaat atcaagatat tcacactagc gtttacgctc accgtggtac
    45721 ttattcagct atcccgtttt atttcgccac ttgccattat ccattccagt tatatctttc
    45781 tggcgtggat gccactgtgc gtaatgctgt caatcttgtt tatctttggc tggcgcggtg
    45841 tcgttcccgt tttatgcggg atgttttgca ccaatctgtg gaactttcat ctctcttttt
    45901 tacagactgc ggtcatgctc ggtagccaga cgtttgtcgt gttgtgtgcc tgcgcaatat
    45961 tacgctggca gctggggacg cgttggcgtt atggattgac cagccgatat gtctggcaac
    46021 gtctgttctg gcttggtttg gtgacgccga tcggcatcaa atgcagcatg tatcttgtgg
    46081 ggagcttctt tgatttcccg ctaaagatat ccaccttttt cggcgatgcg gatgccattt
    46141 tcacggtcgt tgatttgcta agccttttca ccgctgtgct gatttacaac atgcttttct
    46201 actatctcac tcgcatgatt gtaagtcccc actttgcgca gatattgtgg cgcagggata
    46261 tcgcgccgtc gttgggcaaa gagaaacgcg catttacctt aagctggctg gcagcgttaa
    46321 gcgtgctgct acttctgttg tgcacacctt atgaaaacga ctttattgcc ggttacctgg
    46381 tacccgtttt cttcatcatc tttaccctcg gggtcggtaa gcttcgctat ccgtttttaa
    46441 atctcacctg ggctgtttca acgctttgcc ttctgaatta caaccagaac tttttgcaag
    46501 gggtggaaac cgaatattcg ctggcattta ttcttgcggt gctgatttcc tttagcgttt
    46561 gcctgctcta tatggtgcgc atttatcatc gcagtgaatg gcttaatcgc cgctggcatt
    46621 tgcaggcgct gaccgatccg ttaacgctcc tgcccaactt tcgtgcgttg gaacaagcgc
    46681 cggagcaaga ggcgggcaag agtttttgct gcctgcgcat tgataatctt gagtttatga
    46741 gtcgtcatta cggcttaatg atgcgcgttt actgtatccg ctcaatttac cgtacgctgc
    46801 tgccgttgat gcaggaaaac gaaaagttgt atcaattgcc gggtagtgaa ctgctgttag
    46861 tgctgagcgg gccggaaacg gaagggcgac tccagcatat ggttaacatc ctgaatagtc
    46921 ggcaaattca ctggaacaat accgggctgg atatgggcta tggtgctgcc tgggggcgtt
    46981 ttgatggaaa tcaggaaacc ctgcaaccct tgttggggca gttaagctgg ctggcggagc
    47041 aatcctgcgc acatcaccat gtgctggcgc tggatagcag agaggagatg gtttccgggc
    47101 agaccactaa acaggtgcta ttgctgaata ccattcgcac ggcgttagat cagggtgatt
    47161 tactgctcta cgcccagcca attcgcaaca aagagggtga aggttatgat gagatcctcg
    47221 cgcgactgaa gtatgacggc ggcattatga ccccggataa gtttctgccc cttattgctc
    47281 agtttaacct tagcgcgcgt tttgatttgc aagtgctgga atccttgttg aagtggctgg
    47341 caacacaccc ttgcgacaaa aaaggaccgc gcttttcagt caatttaatg ccgctcacgc
    47401 tgctgcaaaa gaatattgcc gggcggatta ttcgtctgtt taagcgttat cacatctccc
    47461 cgcaggcggt cattcttgag atcaccgagg agcaggcgtt ttctaacgca gaaagcagca
    47521 tgtacaacat cgagcagctg cataagtttg gtttccggat tgcgattgat gactttggca
    47581 ccggatatgc caactacgaa cggttaaagc gtttgcaggc tgatatcatc aaaattgatg
    47641 gcgtctttgt gaaagatatt gtcacgaaca cgctggatgc gatgattgtg agatcaatta
    47701 ccgatctggc gaaagcgaag tcattgagtg tggtcgcgga gtttgtcgag acgcaacagc
    47761 agcaggcgct attgcataag ctcggggtgc aatatctgca agggtatttg attggtcgcc
    47821 cgcagccatt agctgattaa caggcgtaaa aaaaccgggg aattatcccc ggctttttta
    47881 tgtatggtct tacagcacca gtgctgcgat tgacgcagac agcacactca ccagggtaga
    47941 gccgtaaacc agcttcagac cgaagcgaga aaccacgtta ccttgctctt cattcaggcc
    48001 tttaactgca cctgcgataa tcccgattga agagaagtta gcgaaggaaa ccaggaacac
    48061 agagatgatg ccttcagcac gcggagagag cgtggaagca attttctgca gatccatcat
    48121 cgcaacgaac tcgttggaaa ccagtttggt cgccatgata ctgcccactt gcagtgcttc
    48181 actggaagga acacccatca cccatgcaat cggatagaag atgtagccca ggatgccctg
    48241 gaaggagatg ctgtagccaa accagccagt aacggtggca aacagtgcgt tcagcgcggc
    48301 gatcagggcg ataaagccaa tcagcatcgc ggcaacgata atggcaactt tgaaacctgc
    48361 cagaatgtat tcacccagca tttcgaagaa gctctgacct tcgtgcaggt tggacatctg
    48421 gatgttttct tcactggcat caacacggta aggattgatc agcgacagca cgataaaggt
    48481 gctgaacatg ttcagtacca gcgcagcaac gacgtatttc ggttccagca tggtcatgta
    48541 tgcaccaacg atggacatcg acacggtgga cattgccgtg gcagccatgg tgtacatacg
    48601 attacgggag attttgccga ggatatcttt ataggcaata aagttttcag actgacccag
    48661 aatcagggag ctgacggcgt taaaggattc cagtttgccc atgccgttga ctttggagag
    48721 caggaaacca attgcgcgga tgatcaccgg caacacgcga atgtgctgga gaataccgat
    48781 cagtgcagag ataaagacga ttgggcacag cactttcagg aagaagaatg ccaggccttg
    48841 atcattcatg ctaccaaaga cgaagttagt cccttcgttg gcaaatccga gcagtttttc
    48901 gaacatttcg gagaagcctt tcacgaagcc taaaccaacg tcggagttca ggaagaacca
    48961 cgccagtaac acttcgataa caagcagttg aataacataa cggatacgaa tttttttgcg
    49021 gtcgctgctt accagcagtg cgagaatcgc aacaacggca agtgccagta caaaatgaag
    49081 gacgcggtcc atatttgctc caaatatgag gcaggttaaa tttccgtgca cattctatgt
    49141 aacatggtta aggaaaacga gatctgacac acactataat gacgtcctgt gacaggcttc
    49201 aaaaatagtg atctgtcata catttctgct atgttgtgta tggaagctga aagttatgta
    49261 aatgtgctaa cattaaacat aaccataaca ttcaagtcat caatcttttc gtagtcataa
    49321 tcctggtcta tcagagaaat caccacaatc catttaaatg aatttgataa tcattctcgt
    49381 ttggcatagc atgaaacata gcaaaggcta tgttttagag gcacaagatg acgaactatc
    49441 gcgttgagag tagcagcgga cgggcggcgc gcaagatgag gctcgcatta atgggacctg
    49501 cgttcattgc ggcgattggt tatatcgatc ccggtaactt tgcgaccaat attcaggcgg
    49561 gtgctagctt cggctatcag ctactgtggg ttgtcgtttg ggccaacctg atggcgatgc
    49621 tgattcagat cctctctgcc aaactaggga ttgccaccgg taaaaatctg gcggagcaga
    49681 ttcgcgatca ctatccgcgt cccgtagtgt ggttctattg ggttcaggca gaaattattg
    49741 cgatggcaac cgacctggcg gaatttattg gtgcggcgat cggttttaaa ctcattcttg
    49801 gtgtttcgtt gttgcagggt gcggtgctga cggggatcgc gactttcctg attttaatgc
    49861 tgcaacgtcg cgggcaaaaa ccgctggaga aagtgattgg cgggttactg ttgtttgttg
    49921 ccgcggctta cattgtcgag ttgattttct cccagcctaa cctggcgcag ctgggtaaag
    49981 gaatggtgat cccgagttta cctacttcgg aagcggtctt cctggcagca ggcgtgttag
    50041 gggcgacgat tatgccgcat gtgatttatt tgcactcctc gctcactcag catttacatg
    50101 gcggttcgcg tcaacaacgt tattccgcca ccaaatggga tgtggctatc gccatgacta
    50161 ttgccggttt tgtcaatctg gcgatgatgg ctacagctgc ggcggcgttc cacttttccg
    50221 gtcatactgg tgttgccgat cttgatgagg cttatctgac gctgcaaccg ctgttaagcc
    50281 acgctgcggc aacggtcttt gggttaagtc tggttgctgc cggactgtcc tcaacggtgg
    50341 tggggacact ggcggggcag gtggtgatgc agggattcat tcgcttccat atcccgctgt
    50401 gggtgcgtcg tacagtcacc atgttgccgt catttattgt cattctgatg ggattagatc
    50461 cgacacggat tctggttatg agtcaggtgc tgttaagttt tggtatcgcc ctggcgctgg
    50521 ttccactgct gattttcacc agtgacagca agttgatggg cgatctggtg aacagcaaac
    50581 gcgtaaaaca gacaggctgg gtgattgtag tgctggtcgt ggcgctgaat atctggttgt
    50641 tggtggggac ggcgctggga ttgtagttga atgagcgtcg catctggcac tattggcgga
    50701 tgcggcgtaa acgccttatc cgccctacgc gttaaagagc cggtttgtag gcctgataag
    50761 acgcgctagc gtcgcatcag gcattgtgtt ccattggcga atgtgtttaa tggcggtgtc
    50821 catgtccgcg gccttttccg cgatgatcgt cacgatcgcg ccagccttca cgatagccac
    50881 gctcatacgc tttacgctta tcccagccac gatgatagcc attatcatga cgccaccagc
    50941 ggtttttacg ccactcataa ttgcgatgcc agtagtcacg gtcgcgccag tgaccaccgt
    51001 cccagtaatt accgtaatga tcgcgatcgc caatttgtaa tttgattgat ggcagtaggg
    51061 tgatttcacc tgcgtttgct gcaagcgggg taaatgccat cagggcggcc gccagaaaca
    51121 gtgacctgaa cattgttatt ctccttcacg atcgaagccg tcagcggcct gttaacgcaa
    51181 tattacgggg aggtaaagcc ccgtatcatc gccataactc ttaaatcata aaggagagca
    51241 ttttttgcta aacgttatcg tggggtggct gttgcttcgg acgattgacc atcatcatcg
    51301 gcttacctgc taataacagc catgccggga gcatgacgat acagagcagc ggtagcaatg
    51361 tggtattggg tacaacgact gccgccataa aaagacttaa ccagtcatcg cgtgttacca
    51421 ccagcacgat gccgagaata gcgcaggaaa cggttattgc cgctggaacg gcgggaacgt
    51481 gctcatgcag catcaaccct aatgccacgc cgacaaacac tgccgggaag attcgcccac
    51541 cgcgaaagcc actggcggca gcaacgacca gggcggtaag tttaatcacc gccagcaaaa
    51601 aataatcgct ggtgctgaaa gcctgatttg ccaccatctg ctgcatctca tccagccctt
    51661 taaacagcga aaccggccca ccaataactc ccagaatacc gagaataaat ccgccaatac
    51721 ctagcacgag caccggattt ttcatttgat gcatcatcgc gtgcaaccgt ggtaagcacc
    51781 atacggcgac cattcctgct gcgatggcaa tcgccgcgac aattgcaccg ctgagaatat
    51841 cggtcatttc catctgtccg taatgcgcaa tgggcagtga aaaatgagga tggaaaaata
    51901 atccggtagt aagtgcacca gctgctgccg ccattaacgg cgcaaagaga cgatcccata
    51961 gcggaacttc actactgcca tttaaggttt gcgaaaatat caacgccgcc gcaacaggag
    52021 tgccaaacag tgcgccgatg gttccggcag aggctaaaat agtccactcc attcggttga
    52081 cgcgcggtaa cagacgagcg ccaatggcaa ccgcaagggc gatattgacg gtcatgatcg
    52141 gatgttccgg ccccaggctg acgccgccag caagaccgag aattaatgct acgataagtc
    52201 caggtagcgc agagggcgga accggtgcgc cgatcagcgg ttcacaggcg gggtctggtc
    52261 cggcatgacc ctggctgaaa cggataacca accccaccgc aatacccgtt agcgttaata
    52321 caccgatgat ccagaggggt gaatcctggg ctatccccag agttcccggc agtcgttgcc
    52381 agagcaaatt ctgtaatacc gaggcgattt tcatcaccac aatcagaata agactggacg
    52441 caatcccaat tgccacggcg gggagcgata ataacaacat ggttctggct cgcggatgga
    52501 gcatgttcta ttccttatgc ggggtcagat acttagtttg cccagcttgc aaaaaggcat
    52561 cgctgcaatt ggtgctgaaa cgataaagta attgtgtgac ccagatcgat atttacaggg
    52621 agcctgcctt tccggcgttg ttgttatgcc cccaggtatt tacagtgtga gaaagaatta
    52681 ttttgacttt agcggagcag ttgaagaatg acaaagtatg cattagtcgg tgatgtgggc
    52741 ggcaccaacg cacgtcttgc tctgtgtgat attgccagtg gtgaaatctc gcaggctaag
    52801 acctattcag ggcttgatta ccccagcctc gaagcggtca ttcgcgttta tcttgaagaa
    52861 cataaggtcg aggtgaaaga cggctgtatt gccatcgctt gcccaattac cggtgactgg
    52921 gtggcaatga ccaaccatac ctgggcgttc tcaattgccg aaatgaaaaa gaatctcggt
    52981 tttagccatc tggaaattat taacgatttt accgctgtat cgatggcgat cccgatgctg
    53041 aaaaaagagc atctgattca gtttggtggc gcagaaccgg tcgaaggtaa gcctattgcg
    53101 gtttacggtg ccggaacggg gcttggggtt gcgcatctgg tccatgtcga taagcgttgg
    53161 gtaagcttgc caggcgaagg cggtcacgtt gattttgcgc cgaatagtga agaagaggcc
    53221 attatcctcg aaatattgcg tgcggaaatt ggtcatgttt cggcggagcg cgtgctttct
    53281 ggccctgggc tggtgaattt gtatcgcgca attgtgaaag ctgacaaccg cctgccagaa
    53341 aatctcaagc caaaagatat taccgaacgc gcgctggctg acagctgcac cgattgccgc
    53401 cgcgcattgt cgctgttttg cgtcattatg ggccgttttg gcggcaatct ggcgctcaat
    53461 ctcgggacat ttggcggcgt gtttattgcg ggcggtatcg tgccgcgctt ccttgagttc
    53521 ttcaaagcct ccggtttccg tgccgcattt gaagataaag ggcgctttaa agaatatgtc
    53581 catgatattc cggtgtatct catcgtccat gacaatccgg gccttctcgg ttccggtgca
    53641 catttacgcc agaccttagg tcacattctg taaatccttc cttttatatc gggaggtaac
    53701 tctcccgata atctttttaa aacatacagt taattcaatt tttctttgtg tcccctcaca
    53761 aggccgacct gcgtcacact tccgtacagc gggattaatt ctccagtaaa tgcattattt
    53821 gtctggtaac ggcgatttgt tttgcacgtt cataatttca ctcgtcaact atcactgaat
    53881 gaggaattaa cgatgagtaa gaaactgatt gccttatgtg cctgcccaat gggcctggct
    53941 cacactttta tggccgctca ggcgttggaa gaagcggcgg tagaagccgg ttatgaagtg
    54001 aaaattgaaa cccagggcgc ggacggtatc cagaatcgcc tgacggcgca ggatatcgcc
    54061 gaagcaacca tcatcatcca ttccgtggca gttaccccgg aagataatga gcgtttcgaa
    54121 tcacgcgacg tttatgaaat cactttgcag gacgcaatta aaaacgctgc gggcatcatc
    54181 aaagaaatcg aagagatgat tgcctctgaa caacagtaat attcgacagg gataattgtt
    54241 atggccatta aaaaacgcag tgcaaccgtt gtgcctggcg catccggtgc ggcagcggca
    54301 gttaagaatc cgcaggcctc taaaagcagc ttctggggtg aactcccgca gcatgtgatg
    54361 tcagggattt cacgcatggt gccgacctta attatgggcg gtgtgatcct cgctttcagc
    54421 cagctgattg cttatagctg gcttaaaatt cctgctgaga tcggcatcat ggatgctctt
    54481 aatagcggga aattctccgg tttcgacctc tccttactga aatttgcctg gctgtcgcag
    54541 tccttcggcg gcgtgttgtt tggttttgcc attccgatgt ttgctgcttt tgtggcgaac
    54601 tctatcggtg gcaaactggc gttcccggct ggttttatcg gcgggttgat gtctacccag
    54661 ccgacgcaac tgctgaactt cgatcccagc actatgcaat gggcgacctc ttcgccggtg
    54721 ccgtccacct tcattggtgc gctgattatt tctatcgtcg cgggttacct tgtgaagtgg
    54781 atgaaccaga aaatccagtt accggatttc ctgttagcgt tcaaaaccac atttttgctc
    54841 ccgattcttt ccgccatttt tgtcatgctg gcgatgtact acgtcatcac cccctttggt
    54901 ggctggatca acggcggtat ccgtactgtg ctgactgccg caggtgagaa gggcgcgctg
    54961 atgtacgcaa tggggatcgc cgcggcgaca gcaatcgacc ttggtggccc gatcaacaaa
    55021 gccgcaggtt ttgttgcctt cagctttacc actgaccacg tattaccggt caccgctcgt
    55081 tctatcgcta tcgttattcc gccgattggt ctggggctgg cgaccattat cgaccgtcgt
    55141 ttaaccggca aacgcctgtt caacgctcaa ctttatccgc agggtaagac cgccatgttc
    55201 ctggcgttta tggggatcag tgagggtgcg attccgtttg cgctggaaag ccccatcacc
    55261 gccattccgt cgtatatggt cggcgctatt gtcggctcaa ccgccgctgt ctggctgggc
    55321 gcagtgcaat ggttcccgga gtccgctatc tgggcatggc cgctggtcac taacctgggc
    55381 gtctatatgg cggggatcgc gctgggggca gtcataactg cgctgatggt ggtgttcctg
    55441 cgtctgatga tgttccgtaa aggcaaattg ttaatcgata gcctgtaaga aggaaaatcc
    55501 agatgacatt actcgcttcg ctgcgcgact ggcttaaggc gcaacaactg gatgcagtgc
    55561 ttctctcctc acggcagaac aaacagccgc atctggggat ctccaccgga tcaggttatg
    55621 tggtgattag ccgtgaaagt gcgcacattc tggtggattc gcgctattac gttgaggtgg
    55681 aagcccgtgc gcaaggctac cagctgcatt tgcttgacgc gacgaacacg cttaccacta
    55741 tcgtcaatca aatcattgcc gatgaacagt tgcaaacgct cggttttgag ggccagcagg
    55801 tgagttggga aaccgcgcac cgctggcagt ctgaactcaa tgcgaaactg gttagcgcca
    55861 cgccggatgt gctgcggcaa atcaaaacgc cagaggaggt ggagaaaatc cgcctcgcct
    55921 gtgggattgc tgatcgcggt gcagagcata ttcgccgctt tattcaggcg gggatgagcg
    55981 agcgcgagat agccgctgaa ctggagtggt ttatgcgcca gcagggcgca gaaaaagcct
    56041 cttttgacac cattgtcgcc agcggctggc gcggggcgct gccgcacggc aaagccagcg
    56101 acaagattgt tgcagcgggc gagtttgtca ctctcgattt tggtgcgctg tatcagggct
    56161 actgctctga tatgacgcgc accttgctgg tgaatggcga aggggtgagc gccgaatctc
    56221 acccgctgtt taacgtctat caaattgtcc tgcaggcaca gctcgcagca atctccgcga
    56281 ttcgccccgg cgtgcgctgc cagcaggttg acgatgccgc gcgccgggtc attacagaag
    56341 caggttatgg cgactatttc ggtcataaca ccggtcacgc tatcggcatt gaagttcatg
    56401 aagatccgcg tttttcaccg cgggacacca cgacgctaca gccaggcatg ttactgaccg
    56461 tggagccggg gatttatttg ccagggcaag ggggcgtgcg catcgaagat gttgtgctgg
    56521 ttaccccgca aggcgcagaa gtgctctacg ccatgccgaa aacagtgttg ctcacgggag
    56581 aggcataatg gatttatcgc tattaaaagc gttgagcgag gcagatgcga tcgcctcctc
    56641 ggaacaggaa gtgcggcaga tcctgctgga agaagcggat cgcctgcaaa aagaagtgcg
    56701 atttgatggt ctgggatcgg tgctgatccg cctgaatgaa tcgacaggtc cgaaggtgat
    56761 gatctgtgcg catatggacg aagtgggatt tatggtgcgc agcatctccc gcgaaggggc
    56821 gattgatgtg ctgccggttg gcaacgtacg catggctgcc cgccagctgc agccggtgcg
    56881 catcaccacc cgtgaagagt gcaaaattcc aggcctgctt gacggcgacc ggcaggggaa
    56941 tgacgtcagc gccatgcgcg tggacattgg tgcgcgctcc tatgacgaag tgatgcaggc
    57001 gggaattcgt cccggcgatc gtgtcacgtt tgataccact tttcaggttc tccctcacca
    57061 gcgagtgatg gggaaagcct ttgatgaccg cctcggttgc tatctgctgg tgacgttact
    57121 gcgcgaactg cacgacgccg aactacctgc ggaagtgtgg ctggtggcaa gttccagcga
    57181 agaggtggga ttacgcggcg ggcaaactgc cacccgcgcg gtgtcgccgg acgtcgccat
    57241 tgtgcttgat accgcctgct gggcgaaaaa ctttgattat ggcgcggcta accatcgcca
    57301 gattggtaac gggccgatgc tggtgttaag cgacaagtcg ctgattgcgc cgccaaaact
    57361 taccgcctgg gtcgaaaccg tggcggcaga aattggcgtg ccgttgcagg cagatatgtt
    57421 cagcaacggc ggcacggacg gcggggcggt gcacttaacc ggcaccggcg tgcccacagt
    57481 ggtgatgggg ccagcaaccc gccatggaca ttgcgccgca tcgattgccg attgccgcga
    57541 cattttgcag atgcagcaac ttttatctgc ccttattcaa cgtcttacgc gtgagacggt
    57601 tgttcaactg acggatttca gatgatctcc tgattaaccc ggagcggtta tgttaacgat
    57661 tcaatttctc tgtcctttgc ctaacggtct acatgctcgt ccggcgtggg aacttaaaga
    57721 acagtgcagc cagtggcaaa gcgaaatcac ttttattaac catcgccaga acgcaaaggc
    57781 agatgcgaaa agctcgctgg cgctgattgg caccggcacc ctatttaatg acagttgcag
    57841 cctgaacatt agcggcagcg atgaagagca ggcgcggcgc gtgctggaag agtacatcca
    57901 ggtgcgcttt atcgacagcg acagcgttca gcctacgcag gcagaactga cggcgcatcc
    57961 gctgccgcgt tcattaagct gccttaaccc ggatttactg tacggcaatg tgctggcaag
    58021 cggcgtcggc gtgggtacgc tgaccctgtt acaaagcgac agcctcgaca gttatcgggc
    58081 aatccccgcc agtgcgcaag attccacccg gctggagcac agcctggcaa cgcttgccga
    58141 gcaactgaac cagcaattgc gtgagcgtga cggcgaaagc aaaactatcc tcagcgccca
    58201 tttgtcgctg attcaggatg atgaatttgc aggcaatatc cgtcgcctga tgacagaaca
    58261 gcatcagggg ctgggggcgg cgatcatcag caatatggag caggtttgcg ccaaactttc
    58321 tgcctctgcc agcgattatc tgcgcgaacg tgttagcgac attcgcgata tcagcgaaca
    58381 gttgctgcat atcacctggc cggaactgaa gccgcgcaac aagctggtgc ttgaaaaacc
    58441 gaccattctg gtggctgaag atttaacccc aagccagttt ttgagccttg atttgaaaaa
    58501 tcttgcgggc atgattctgg agaaaaccgg gcgcacctcg catacactga ttctggcccg
    58561 tgcctcggcg atcccggtac tgagtggctt gccgctggat gcgattgccc gttatgccgg
    58621 gcaacctgca gtgcttgacg cccagtgcgg cgtgctggcg attaacccga atgacgcggt
    58681 gagcggttat tatcaggtcg cgcagacgct ggcggataaa cgccaaaaac aacaggcgca
    58741 ggctgccgcg cagctggcct attcccgtga taacaagcgt attgatattg cggcgaatat
    58801 cggcaccgct ctggaagcgc caggcgcgtt tgccaacggc gcggaaggcg ttgggctgtt
    58861 ccgtaccgaa atgctctata tggatcgcga cagcgcgccg gacgagcagg agcaatttga
    58921 agcctaccag caggtgctac tggcggcggg cgacaagccg attatcttcc gcacgatgga
    58981 catcggcggc gataaaagca ttccttatct gaatattccc caggaagaga acccgttcct
    59041 cggctatcgc gcggtacgta tttacccgga atttgctggc ctgttccgca ctcaactgcg
    59101 ggccattttg cgcgccgcca gtttcggcaa cgcccagttg atgatcccga tggttcacag
    59161 cctcgatcag atcttatggg tgaaaggcga gatccaaaaa gcgatcgttg agcttaagcg
    59221 cgatggcctg cgtcatgcag agacgattac gcttgggatc atggtggaag ttccgtcggt
    59281 gtgctacatc atcgaccact tctgcgatga ggtcgatttc ttcagtatcg gctccaacga
    59341 tatgacccag tatctgtatg cggtcgatcg taataacccg cgcttatcgc cgctatataa
    59401 cccgattacg ccatcgttcc tgcgcatgtt gcagcaaata gttaccactg cgcatcagcg
    59461 gggcaaatgg gtaggcattt gcggtgaact gggcggtgaa agccgttatc tgccgctact
    59521 gcttgggctg ggcctggatg agctgagtat gagtagcccg cgtattccgg cggtgaaaag
    59581 ccagcttcgt caactggata gcgaggcgtg tcgggaactg gcgcgtcagg catgtgaatg
    59641 ccgcagtgcg caggaaattg aagcgttact caccgccttt acgccggaag aagacgttcg
    59701 cccactgctg gcgctggaga atatctttgt tgatcaggat tttagcaata aagagcaggc
    59761 gatccagttc ctgtgcggca acctcggcgt taacgggcgc actgaacatc cgtttgagct
    59821 ggaagaagat gtctggcagc gggaagagat tgttaccacc ggcgttggtt ttggcgtagc
    59881 gatcccgcac accaaatctc agtggatccg tcattccagt atcagcattg cccggctggc
    59941 gaaaccgatt gactggcagt cagaaatggg cgaagtcgaa ctggtgatca tgctgacgct
    60001 gggtgctaac gaagggatga atcatgtgaa agtcttctcg cagctggcgc gtaaactggt
    60061 gaataaaaac ttccgccagt cgttgtttgc cgcgcaagat gcacaaagta tcctgacgct
    60121 gctggaaaca gaattaacct tctgacgtta gccctgaaaa cgggcgctgt actctcccgg
    60181 cgtcagacca aactgacgcc ggaaaacgcg acaaaaatag tcgctatccg gaaaaccgca
    60241 acgctgtgcc acttcatgaa ttgacagatg atatttctgc aaaatcatcc tcgccttcgc
    60301 cattcgcacc caacgcacat actcgataaa acgcatcgtt ccatgctgag caaacagttt
    60361 tgacagatga ttgggcgtga tattaaaaaa ctgcgccacg ctctcgcggg tgagcggctg
    60421 ggcataatta tcctgaaccc agttacaaat gctgtgatag agaaaatcgc cgcgcggctg
    60481 cgaattgccc ggcggcatat tcaccacgct acggcaaaga tgcagcaaac ttaacaccag
    60541 cggctgaatg atattttgct ccagcggcga acggctaaga tgggtgaggg cggtaagcat
    60601 cgcttcacct tcaccgcgtt gcaggctggg aagctggatc tggcgagcag gatgcaacaa
    60661 cgacgtggtg cggctatcgt aaaacgataa cccaagccag gtcggagcga acaccaggct
    60721 taacagcatc accggtttgt tgttgaccgg taaattagcc gcgcgcgccg gaataaacag
    60781 catttctcct tccaccaaac gttgctgctt tgcttccagc agattgccgt attcgccgcg
    60841 cagcacgata tccagacggg gaaaatcaat acataaactg cccaccggca acgaggcagg
    60901 ctggctggca aaccagacgc gccccagtag ttgcgggtta agcaccaggc cgctgaacag
    60961 atcggcgaaa aattgttgat cggcgggcaa gcctggtgct ttcatgaaag ttcccgatta
    61021 aagatgcatt aactggcgaa attcttttac tttgctgcgg ctgactggca cttcaaaatc
    61081 cagatctttc agccgcagaa tataggtgtt attaaaccac ggttcgattt cgcggatttt
    61141 gttcagattg acacaaaatg agcgatggca gcggaaaaaa tgcgacggcg gcagtttgct
    61201 gcaaaattcg gtaatgttca tcggcattac gtaggattca cgccgcgtat agacaaaggt
    61261 cattttctcg tgcgcttcgg cgtaatagat atcgttaatt ggcgtgacga ttattcgctc
    61321 atctttcacc agattaatgg tgtcattctc acgcgttacc gtcgcggcag gcgtgctgct
    61381 ggtctgctgt tgttgccagg ccgcttccag tttttgcagc atcccggtaa tgcgtgactc
    61441 ctgatacggt ttgagaatgt agtcaaacgc ctccagttca aacgcttcta ccgcatgttc
    61501 tttccacgcg gtgatgaaca caataaacgg tttatgggcg aactggctga tgttttgcgc
    61561 cagcaacaca ccatccagcg acggaatatt gatatccaga aaaatggcgt cgacgcggtt
    61621 atgctgcaaa aacttcaaca cgtccagacc gtcgtcaaag gtgccgacaa tctccatctg
    61681 gctgtgctct ttaattagcc agctcagttc ctgttgtgcc aggaattcgt cttcaacaat
    61741 gatgactttc acaatatcac tccggctcaa agcaataacg tagcctgtga ggcgactggg
    61801 gtgcgttggt taggaatgta aaacgcaatt tccgttcctg gttccaggcg gcggatatgc
    61861 agcccctcgc catacaataa cttcacgcga tgatgaacat tcagcaggcc aattttattg
    61921 cccggcattt cattcgcttc gacccgctca atcacctttg gatcgatgcc gtgcccggta
    61981 tcccgcaccg caatgcgtac ccgattgccg cactctgcaa cgctgatggt gacaacgcct
    62041 ttacctttgc aaggctggat accgtggaca atggcgttct ccaccaacgg ctggatcagc
    62101 aggctgggaa tgcagcaatt cacctcttca tcaatatcat agataaccgt cagcttgtcg
    62161 ccaaagcgag cctgctcaat ggcaatatag tctttaattt gatacagctc ttttttgata
    62221 tcgatttgct catcgtcttt taattcaatg ttatagcgca gataacgcga cagattaaag
    62281 atcaactggc gagcggtatc cggattcaga cggattgacg atgaaatagc gttcagagcg
    62341 ttaaacagaa aatggggatt aattttgctt tgcagggcgc gaagctctgc cttatttgcc
    62401 atttcacgta gctgctcggc gcgtgaaacc tccagttgcg tggagatgat ttgcgacaga
    62461 ccgaccgcca tttcctgtaa tgacgaggtg atctgatgcg cgtggcagta gtaaattttc
    62521 agcgttccgg tcacgacccc tttctcccac aatgggatca ccagcatgga atgaatctct
    62581 ggtgtgcggt gggcttcatc attgttttta atgatgattt ttccgtaatt catcgcctga
    62641 cgggtagtcg ggctaatgaa gtcatcgcca ttctgatagt tatgttcacc cacgccaaca
    62701 taggccagca catgatcggt attagtaatc gccaccgcat cggcgtgaat gtcatcgcgg
    62761 ataatttcgc agaccttgcg taatgactcg ctattgacat ggcgaaacag cggtagcgtt
    62821 ttgttggcga tatccagcgc cagcttggcc tgccgcgcgg cgctggcctc tttttcgccc
    62881 tcaacgcttt gcaccagaag cacaataaag ccgatacaga cgctaccgag gatcattgga
    62941 atgccgattt tagagacgat atcgatcccc aacgcggtag ttggtgccca gacaattacc
    63001 agaatcatgg tcaacgtctc acacaacatg ccgccgagaa taccgacgcg ccagcgctgt
    63061 gctttgggga ttttcaggtt gatccagccc gatatacaac ccgccagaat gctggtgata
    63121 aagcagggga tcgccgtcac gccgccgata tcaattaaat accggtgaat acccgcaatc
    63181 acgccggtaa tgatgcctac ccacgggcca aacagaatcc cgccggacat caccgcgata
    63241 atacgtacgt tcaccagcga gccttctacg ggaacgccgg accaggtgct gaacagggcg
    63301 aacagcgaga aaatggcggt aacggcgagc aattcttttg gggagtgcgc cgacttgtgc
    63361 aacagttcgc gaaacaggcg gatacggatg agaaagaaca ggcagataag cattaacgct
    63421 gcccgatcga agaccgccag cagcatgttg aatatttcgt gcacgggtga ctctgaataa
    63481 ggattaaacc tctatgatag gtaacctgaa ggctgatgac cagcaggccg tttttgagga
    63541 aaaaccataa atttaaaagg ggattgaaag ataaatggtg tattagcaat gtatctcttg
    63601 ataatgcgct tctggatggt aaatt
//
LOCUS       DAJOSY010000014        55959 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJOSY010000014 DAJOSY010000000
VERSION     DAJOSY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.014"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..2234
                     /gene="fhuA"
                     /locus_tag="OHN27_000966"
     CDS             <1..2234
                     /gene="fhuA"
                     /locus_tag="OHN27_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCQ2285453.1"
                     /translation="KTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITVTAAP
                     APQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEALSYT
                     PGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLERAEI
                     MRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALDDDGV
                     YSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGYYGWL
                     PKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLRFAEN
                     KTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDTQLQS
                     KFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDPANSG
                     PYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQFTWR
                     GGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRPIVVT
                     GAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYTDAEY
                     TTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANSFKVG
                     SYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVVATAT
                     FRF"
     gene            2285..3082
                     /gene="fhuC"
                     /locus_tag="OHN27_000967"
     CDS             2285..3082
                     /gene="fhuC"
                     /locus_tag="OHN27_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCQ2285454.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            3082..3972
                     /gene="fhuD"
                     /locus_tag="OHN27_000968"
     CDS             3082..3972
                     /gene="fhuD"
                     /locus_tag="OHN27_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCQ2285455.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGERPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            3969..5951
                     /gene="fhuB"
                     /locus_tag="OHN27_000969"
     CDS             3969..5951
                     /gene="fhuB"
                     /locus_tag="OHN27_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCQ2285456.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(6109..7389)
                     /gene="hemL"
                     /locus_tag="OHN27_000970"
     CDS             complement(6109..7389)
                     /gene="hemL"
                     /locus_tag="OHN27_000970"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCQ2285457.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            7614..9035
                     /gene="clcA"
                     /locus_tag="OHN27_000971"
     CDS             7614..9035
                     /gene="clcA"
                     /locus_tag="OHN27_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCQ2285458.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            9059..9124
                     /locus_tag="OHN27_000972"
     CDS             9059..9124
                     /locus_tag="OHN27_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285459.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            9117..9461
                     /gene="erpA"
                     /locus_tag="OHN27_000973"
     CDS             9117..9461
                     /gene="erpA"
                     /locus_tag="OHN27_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCQ2285460.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(9508..10131)
                     /locus_tag="OHN27_000974"
     CDS             complement(9508..10131)
                     /locus_tag="OHN27_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCQ2285461.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(10169..10969)
                     /gene="btuF"
                     /locus_tag="OHN27_000975"
     CDS             complement(10169..10969)
                     /gene="btuF"
                     /locus_tag="OHN27_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCQ2285462.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(10962..11660)
                     /gene="mtnN"
                     /locus_tag="OHN27_000976"
     CDS             complement(10962..11660)
                     /gene="mtnN"
                     /locus_tag="OHN27_000976"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCQ2285463.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            11744..13261
                     /gene="dgt"
                     /locus_tag="OHN27_000977"
     CDS             11744..13261
                     /gene="dgt"
                     /locus_tag="OHN27_000977"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCQ2285464.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFI
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSAR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            13391..14815
                     /gene="degP"
                     /locus_tag="OHN27_000978"
     CDS             13391..14815
                     /gene="degP"
                     /locus_tag="OHN27_000978"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCQ2285465.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            14970..16127
                     /gene="cdaR"
                     /locus_tag="OHN27_000979"
     CDS             14970..16127
                     /gene="cdaR"
                     /locus_tag="OHN27_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCQ2285466.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(16216..16602)
                     /locus_tag="OHN27_000980"
     CDS             complement(16216..16602)
                     /locus_tag="OHN27_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCQ2285467.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(16764..17576)
                     /gene="yaeI"
                     /locus_tag="OHN27_000981"
     CDS             complement(16764..17576)
                     /gene="yaeI"
                     /locus_tag="OHN27_000981"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCQ2285468.1"
                     /translation="MISRRCLLLAAAATLTAGSGFGYMRYWEPGWLELTHQRIAFFKD
                     KAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     NVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(17630..18454)
                     /gene="dapD"
                     /locus_tag="OHN27_000982"
     CDS             complement(17630..18454)
                     /gene="dapD"
                     /locus_tag="OHN27_000982"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCQ2285469.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(18485..21157)
                     /gene="glnD"
                     /locus_tag="OHN27_000983"
     CDS             complement(18485..21157)
                     /gene="glnD"
                     /locus_tag="OHN27_000983"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCQ2285470.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDVDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVHNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(21219..22013)
                     /gene="map"
                     /locus_tag="OHN27_000984"
     CDS             complement(21219..22013)
                     /gene="map"
                     /locus_tag="OHN27_000984"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCQ2285471.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            22381..23106
                     /gene="rpsB"
                     /locus_tag="OHN27_000985"
     CDS             22381..23106
                     /gene="rpsB"
                     /locus_tag="OHN27_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCQ2285472.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            23364..24215
                     /gene="tsf"
                     /locus_tag="OHN27_000986"
     CDS             23364..24215
                     /gene="tsf"
                     /locus_tag="OHN27_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCQ2285473.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            24362..25087
                     /gene="pyrH"
                     /locus_tag="OHN27_000987"
     CDS             24362..25087
                     /gene="pyrH"
                     /locus_tag="OHN27_000987"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCQ2285474.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            25379..25936
                     /gene="frr"
                     /locus_tag="OHN27_000988"
     CDS             25379..25936
                     /gene="frr"
                     /locus_tag="OHN27_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCQ2285475.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            26028..27224
                     /gene="ispC"
                     /locus_tag="OHN27_000989"
     CDS             26028..27224
                     /gene="ispC"
                     /locus_tag="OHN27_000989"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCQ2285476.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFAQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            27413..28171
                     /gene="ispU"
                     /locus_tag="OHN27_000990"
                     /gene_synonym="uppS"
     CDS             27413..28171
                     /gene="ispU"
                     /locus_tag="OHN27_000990"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCQ2285477.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            28184..29041
                     /gene="cdsA"
                     /locus_tag="OHN27_000991"
     CDS             28184..29041
                     /gene="cdsA"
                     /locus_tag="OHN27_000991"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCQ2285478.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            29053..30405
                     /gene="rseP"
                     /locus_tag="OHN27_000992"
     CDS             29053..30405
                     /gene="rseP"
                     /locus_tag="OHN27_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCQ2285479.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPSVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            30435..32867
                     /gene="bamA"
                     /locus_tag="OHN27_000993"
     CDS             30435..32867
                     /gene="bamA"
                     /locus_tag="OHN27_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCQ2285480.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            32989..33474
                     /gene="skp"
                     /locus_tag="OHN27_000994"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             32989..33474
                     /gene="skp"
                     /locus_tag="OHN27_000994"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCQ2285481.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            33478..34503
                     /gene="lpxD"
                     /locus_tag="OHN27_000995"
     CDS             33478..34503
                     /gene="lpxD"
                     /locus_tag="OHN27_000995"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCQ2285482.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            34608..35063
                     /gene="fabZ"
                     /locus_tag="OHN27_000996"
     CDS             34608..35063
                     /gene="fabZ"
                     /locus_tag="OHN27_000996"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCQ2285483.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            35067..35855
                     /gene="lpxA"
                     /locus_tag="OHN27_000997"
     CDS             35067..35855
                     /gene="lpxA"
                     /locus_tag="OHN27_000997"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCQ2285484.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            35855..37003
                     /gene="lpxB"
                     /locus_tag="OHN27_000998"
     CDS             35855..37003
                     /gene="lpxB"
                     /locus_tag="OHN27_000998"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCQ2285485.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LCQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            37000..37596
                     /gene="rnhB"
                     /locus_tag="OHN27_000999"
     CDS             37000..37596
                     /gene="rnhB"
                     /locus_tag="OHN27_000999"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCQ2285486.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            37633..41115
                     /gene="dnaE"
                     /locus_tag="OHN27_001000"
     CDS             37633..41115
                     /gene="dnaE"
                     /locus_tag="OHN27_001000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCQ2285487.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            41128..42087
                     /gene="accA"
                     /locus_tag="OHN27_001001"
     CDS             41128..42087
                     /gene="accA"
                     /locus_tag="OHN27_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCQ2285488.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            42186..44327
                     /gene="ldcC"
                     /locus_tag="OHN27_001002"
     CDS             42186..44327
                     /gene="ldcC"
                     /locus_tag="OHN27_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCQ2285489.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            44384..44773
                     /locus_tag="OHN27_001003"
     CDS             44384..44773
                     /locus_tag="OHN27_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCQ2285490.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            44838..46136
                     /gene="tilS"
                     /locus_tag="OHN27_001004"
     CDS             44838..46136
                     /gene="tilS"
                     /locus_tag="OHN27_001004"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCQ2285491.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(46185..46445)
                     /gene="rof"
                     /locus_tag="OHN27_001005"
     CDS             complement(46185..46445)
                     /gene="rof"
                     /locus_tag="OHN27_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCQ2285492.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(46432..46632)
                     /locus_tag="OHN27_001006"
     CDS             complement(46432..46632)
                     /locus_tag="OHN27_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCQ2285493.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            46798..47343
                     /locus_tag="OHN27_001007"
     CDS             46798..47343
                     /locus_tag="OHN27_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCQ2285494.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            47340..47762
                     /gene="arfB"
                     /locus_tag="OHN27_001008"
     CDS             47340..47762
                     /gene="arfB"
                     /locus_tag="OHN27_001008"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCQ2285495.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            47776..48486
                     /gene="nlpE"
                     /locus_tag="OHN27_001009"
                     /gene_synonym="cutF"
     CDS             47776..48486
                     /gene="nlpE"
                     /locus_tag="OHN27_001009"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCQ2285496.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPLTPMTLRGMYFYMADVA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYLNKDCSSLGQ"
     gene            complement(48660..49484)
                     /locus_tag="OHN27_001010"
     CDS             complement(48660..49484)
                     /locus_tag="OHN27_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCQ2285497.1"
                     /translation="MDKPKAYCRLFLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(49538..51256)
                     /gene="proS"
                     /locus_tag="OHN27_001011"
     CDS             complement(49538..51256)
                     /gene="proS"
                     /locus_tag="OHN27_001011"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCQ2285498.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(51368..52075)
                     /gene="tsaA"
                     /locus_tag="OHN27_001012"
     CDS             complement(51368..52075)
                     /gene="tsaA"
                     /locus_tag="OHN27_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCQ2285499.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(52072..52476)
                     /gene="rcsF"
                     /locus_tag="OHN27_001013"
     CDS             complement(52072..52476)
                     /gene="rcsF"
                     /locus_tag="OHN27_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCQ2285500.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(52594..53409)
                     /gene="metQ"
                     /locus_tag="OHN27_001014"
     CDS             complement(52594..53409)
                     /gene="metQ"
                     /locus_tag="OHN27_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCQ2285501.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(53449..54102)
                     /gene="metI"
                     /locus_tag="OHN27_001015"
     CDS             complement(53449..54102)
                     /gene="metI"
                     /locus_tag="OHN27_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCQ2285502.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(54095..55126)
                     /gene="metN"
                     /locus_tag="OHN27_001016"
     CDS             complement(54095..55126)
                     /gene="metN"
                     /locus_tag="OHN27_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCQ2285503.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISSGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            55314..55886
                     /gene="gmhB"
                     /locus_tag="OHN27_001017"
     CDS             55314..55886
                     /gene="gmhB"
                     /locus_tag="OHN27_001017"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCQ2285504.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        13238 a        13885 c        15352 g        13484 t
ORIGIN      
        1 ccaaaactgc tcagccaaaa cactcactgc gtaaaatcgc agttgtagta gccacagcgg
       61 ttagcggcat gtctgtttat gcacaggcag cggttgaacc gaaagaagac actatcaccg
      121 ttaccgctgc acctgcgccg caagaaagcg catgggggcc tgctgcaact attgcggcgc
      181 gacagtcagc taccggcact aaaaccgata cgccgattca aaaagtgcca cagtctattt
      241 ctgttgtgac cgccgaagag atggcgctgc atcagccgaa gtcggtaaaa gaagcgctta
      301 gctacacgcc gggtgtctct gttggtacgc gtggcgcatc caacacctat gaccacctga
      361 tcattcgcgg ttttgcggca gaaggccaaa gccagaataa ctatctgaat ggcctgaagt
      421 tgcagggcaa cttctataac gatgcggtca ttgatccgta tatgctggaa cgcgctgaaa
      481 ttatgcgtgg cccggtttcc gtgctttacg gtaaaagcag tcctggcggt ctgttgaata
      541 tggtcagcaa gcgtccgacc accgaaccgc tcaaagaagt tcagtttaaa gccggtactg
      601 acagcctgtt ccagactggt tttgacttta gcgatgcgct ggatgatgac ggcgtttact
      661 cttatcgcct gaccggtctt gcgcgttctg ccaatgccca gcagaaaggg tcagaagagc
      721 agcgttatgc tattgcaccg gcgttcacct ggcgtccgga tgataaaacc aatttcacct
      781 tcctttctta cttccagaac gagccggaaa ccggttatta cggctggttg ccgaaagagg
      841 gaaccgttga gccgctgccg aacggtaagc gtctgccgac agactttaat gaaggggcga
      901 agaacaacac ctattctcgt aatgagaaga tggtcggcta cagcttcgat cacgaattta
      961 acgacacctt tactgtgcgt cagaacctgc gctttgctga aaacaaaacc tcgcaaaaca
     1021 gcgtttatgg ttacggcgtc tgctccgatc cggcgaatgc ttacagcaaa cagtgtgcgg
     1081 cattagcgcc agcggataaa ggccattatc tggcacgtaa atacgtcgtt gatgatgaga
     1141 agctgcaaaa cttctccgtt gatacccagt tgcagagcaa gtttgccact ggcgatatcg
     1201 accacaccct gctgaccggt gtcgacttta tgcgtatgcg taatgacatc aacgcctggt
     1261 ttggttacga cgactctgtg ccactgctca atctgtacaa tccggtgaat accgatttcg
     1321 acttcaatgc caaagatccg gcaaactccg gcccttaccg cattctgaat aagcagaaac
     1381 aaacgggcgt ttatgttcag gatcaggcgc agtgggataa agtgctggtc accctgggcg
     1441 gtcgttatga ctgggcagat caagaatctc ttaaccgcgt tgccgggacg accgataaac
     1501 gtgatgacaa acagtttacc tggcgtggtg gtgttaacta cctgtttgat aatggtgtaa
     1561 caccttactt cagctatagc gaatcgtttg aaccttcttc gcaagttggg aaggatggta
     1621 atattttcgc accgtctaaa ggtaagcagt atgaagtcgg cgtgaaatat gtaccggaag
     1681 atcgtccgat tgtagttact ggtgccgtgt ataatctcac taaaacaaac aacctgatgg
     1741 cggaccctga gggttccttc ttctcggttg aaggtggcga gatccgcgct cgtggcgtag
     1801 aaatcgaagc gaaagcggcg ctgtcggcga gtgttaacgt agtcggttct tatacttaca
     1861 ccgatgcgga atacaccacc gatactacct ataaaggcaa tacgcctgca caggtgccaa
     1921 aacacatggc ttcgttgtgg gctgactaca ccttctttga cggtccgctt tcaggtctga
     1981 cgctgggcac cggtggtcgt tatactggct ccagttatgg tgatccggct aactccttta
     2041 aagtgggaag ttatacggtc gtggatgcgt tagtacgtta tgatctggcg cgagtcggca
     2101 tggctggctc caacgtggcg ctgcatgtta acaacctgtt cgatcgtgaa tacgtcgcca
     2161 gctgctttaa cacttatggc tgcttctggg gcgcagaacg tcaggtcgtt gcaaccgcaa
     2221 ccttccgttt ctaatttctc ttttggggca cggatttccg tgcccatttc acaagttggc
     2281 tgttatgcag gaatacacga atcattccga taccactttt gcactgcgta atatctcctt
     2341 tcgtgtgccc gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg ccgggaaagt
     2401 gaccggtctg attggtcaca acggttctgg taaatccact ctgctcaaaa tgcttggccg
     2461 tcatcagccg ccgtcggaag gggagattct tcttgatgcc caaccgctgg aaagctggag
     2521 cagcaaagcg tttgcccgca aagtggctta tttgccgcag cagcttcctc cggcagaagg
     2581 gatgaccgtg cgtgaactgg tggcgattgg tcgttacccg tggcatggcg cgctggggcg
     2641 ctttggggcg gcagatcgcg aaaaagtcga ggaagctatc tcgctggttg gcttaaaacc
     2701 gctggcgcat cggctggtcg atagtctctc tggcggcgaa cgtcagcggg cgtggatcgc
     2761 catgctggtg gcgcaggata gccgttgtct gttgctcgac gaaccgacct cggcgctgga
     2821 tatcgcccac caggttgatg tgctgtcgct ggtgcaccgt ttaagtcagg agcgtggcct
     2881 gacggtcatt gccgtgttgc acgatatcaa tatggcggca cgctactgtg attatctggt
     2941 cgccctgcgc ggcggtgaaa tgattgctca gggaacgcct gcggaaatta tgcgcggcga
     3001 aaccctcgaa atgatttatg gcatcccgat gggtattttg ccgcatccgg cgggtgctgc
     3061 acctgtgagt tttgtttatt gatgagcggc ttacctctta tttcgcgccg tcgactgtta
     3121 acggcgatgg cgctttcccc gttgttatgg cagatgaata ccgcccacgc ggcggctatt
     3181 gatcccaatc gtattgtggc gctggagtgg ttgccggtgg aattactgct ggcgctcggc
     3241 atcgtgcctt acggcgtggc ggataccatc aactatcgcc tgtgggtcag cgaaccacca
     3301 ttgccggact cagtgatcga cgtcggtttg cgcacagaac ctaaccttga actgctgacc
     3361 gaaatgaaac catcgtttat ggtctggtcg gcaggatatg gcccttcacc agaaatgctg
     3421 gctcgtattg cgccggggcg tggatttaac ttcagcgatg gcaaacagcc gctggcgatg
     3481 gcgcgtaagt cactgacgga aatggcagat ttacttaacc tgcaaagcgc agcagaaacg
     3541 catttagcgc actatgaaga ctttatccgc agcatgaaac cccgctttgt gaagcgtggt
     3601 gaacgcccgt tattgctgac gacgcttatc gatccgcgcc atatgctggt tttcggtcca
     3661 aacagcttgt ttcaggaaat tcttgatgag tacggcatcc caaatgcctg gcaaggggaa
     3721 accaacttct ggggcagtac cgccgtcagc atcgatcgtc tggcggcgta taaagatgtt
     3781 gatgtgctct gttttgatca cgacaacagc aaagacatgg acgcgctaat ggcaaccccg
     3841 ctgtggcagg ccatgccgtt tgtccgcgcc ggacgctttc agcgcgtacc cgcagtctgg
     3901 ttttatggtg cgacgctctc ggcaatgcac tttgtgcgcg ttctggataa cgccatcgga
     3961 ggtaaagcgt gagtaaacga attgcgcttt ttccggcgtt attgctggcg ttgctggtga
     4021 ttgccgctac ggcgctgacc tggatgaact tctcgcaggc gctgccgcgt agccagtggg
     4081 cgcaggctgc ctggtcgccg gatattgacg tcatcgagca gatgattttt cactacagct
     4141 tgttgccgcg tctggcgatt tcgctgctgg tgggcgcggg cctggggctg gtgggcgtgc
     4201 tgtttcagca agtgctgcgt aaccctctgg cggaaccgac gacgttgggc gttgcaacag
     4261 gcgcgcaact ggggattacc gtcactacgc tctgggcgat cccaggtgct atggcgagcc
     4321 agtttgctgc gctggcaggg gcttgcgttg ttggcttaat tgtctttggc gtcgcgtggg
     4381 ggaaacggct gtcgccggta acgctgattc tcgcggggct ggtagtgagc ctttattgtg
     4441 gggcaatcaa tcagttactg gttatcttcc atcatgacca gttgcaaagc atgttcctgt
     4501 ggagcaccgg aacgctgacg caaaccgact ggggcggcgt tgagcgttta tggccgcagt
     4561 tgctgggcgg cgtgatgctg acgttactgc tacttcgtcc gttaaccctg atggggcttg
     4621 atgatggcgt ggcgcgcaat ctcgggctgg ccttgtcgct cgcgcgtctg gcggcgctgt
     4681 cgctggcgat agtcatcagt gcgctgctgg tgaacgcagt ggggattatc ggctttatcg
     4741 ggttgttcgc gccactgctg gcaaaaatgc tgggggcgcg gcgtttgttg ccacggttga
     4801 tgctggcgtc gctgattggt gcgttaattc tgtggctttc tgatcaaatc atcctctggc
     4861 tgactcgcgt gtggatggaa gtttccaccg gttcggtcac tgcgttgatc ggtgcgccgc
     4921 tgctactgtg gctgttgccg cgtttacgca gcattagcgc gccggatatg aaggtcaacg
     4981 atcgtgtcgc ggctgaacgc caacatgtgc tggcgtttgc cctcgcgggc ggcgtgctgc
     5041 tgttgatggc tgtggtggtg gcgctgtcgt ttggtcgtga tgcgcacggc tggacgtggg
     5101 cgagcggggc gttgctcgag gatttaatgc cctggcgctg gccgcgaatt atggcggcgc
     5161 tgtttgcggg cgtcatgctg gcggtggcgg gctgtattat tcagcgactg accggaaacc
     5221 cgatggcaag cccggaagtg ctggggatta gctccggcgc ggcgtttggc gtggtgttga
     5281 tgctgtttct ggtgccgggt aatgcctttg gctggctgtt acctgcaggc agtctcggcg
     5341 cggcggtgac gctgttgatc attatgatcg ccgccggccg cggtggattt tccccacacc
     5401 gtatgttact ggcggggatg gcgttaagca ccgcgttcac catgcttttg atgatgttgc
     5461 aggcaagtgg tgacccgcga atggcgcaag tgctgacctg gatttccggt tcgacctaca
     5521 acgcgaccga tgcgcaggtc tggcgcaccg gaattgtgat ggtgattttg ctggcgatta
     5581 ccccgctgtg ccgccgctgg ctgaccattt taccgctggg tggtgatacc gcccgagccg
     5641 taggaatggc gctgacgccg acgcgaattg cgctgctgct gttagcggct tgcctgacgg
     5701 cgaccgcgac gatgactatt ggaccgttga gttttgttgg tttaatggca ccgcatattg
     5761 cgcggatgat gggttttcga cggacgatgc cacacatcgt aatttcggcg ctggtgggtg
     5821 gtttactgct ggtgttcgct gactggtgtg ggcggatggt gctgtttccg ttccagatcc
     5881 cggcggggct gctgtcgacc tttatcggcg cgccatattt tatctatttg ctgagaaagc
     5941 agagccgtta atttttccat ataacggccc tgtacgcctg gacggataaa gcgtttacgc
     6001 gacaacggat gcccgatgcg acgctggcgc gtcttatcgg gcctacaaac ggccccgaat
     6061 cgtaggtcgg ataaggcgtt cacgccgcat ccgacaaacc atgctggatc acaacttcgc
     6121 aaacacccga cgtgcagcat cgatggtgtt attgatatct tccatgctgt gcgccacgga
     6181 cataaagccc gcttcaaacg ctgacggtgc caggtaaaca ccttcgtcca gcatcatatg
     6241 gaagaaacgc ttaaagcgtt ccacgtcaca ggccatcaca tcctgatagc acgtcacgga
     6301 ctcggcgtcg gtaaagaaaa taccgaacat gccgccaacg tggttaacga ccagcggaat
     6361 tccggcttct tctgccgctt ccagcagacc ttctgccaga cgtgttgtca gctcatccag
     6421 cgtttcgtga acgcccggct gcgcgacttc attcagacag gcgaaacccg ctgccatcgc
     6481 aatcgggtta ccggaaagcg tacccgcctg atagaccgga cccgtcgggg ccagcgcatc
     6541 cattacatca cgacgaccac cgaatgcgcc taccggcatt ccaccgccga tgattttgcc
     6601 gaggcaggtt aaatctggca ctacgccgta ataatcctgt gcgccagcta gcgctacgcg
     6661 gaaaccggtc atcacttcat cgatgatcag caacgcgcca aattcgtcgc acagcgcgcg
     6721 cagacctggc aggaactctg gcagcggcgg aacacagttc atattgcctg ccaccggctc
     6781 gacgataata caggcaatct cttgcgggta ttgctcaaat gcggcgcgta cagaagccag
     6841 atcattataa gtacaggtta aggtatgttt ggcgaaatct gccggaacgc ccggcgagtt
     6901 tggctggcct aacgtgagtg cgccagaacc ggctttcacc agcaggcagt cagcgtgacc
     6961 atggtaacac ccttcaaatt taataatttt gtcgcgaccg gtaaaaccac gggccaggcg
     7021 gatggcgctc atggtcgctt cagtgccgga gttcaccatg cgcaccatat ccatggtcgg
     7081 gaccagttcg gtcaccagtt gcgccatttt cacttccatt tcggttggtg caccaaagct
     7141 taaaccacgc tcggcggctt caatcacggc attgcggatt gccggatggt tatggcccag
     7201 caccatcggc ccccaggaac cgacataatc gatataggct ttgccatcaa catcgtacag
     7261 ataagcgccg tccgcttttt cgataaacag tggagtgccg cccacgccag taaaggcgcg
     7321 aacaggggag ttcacaccgc cagggatcag ctcgcgcgct gcgctgtaaa gattttcaga
     7381 cttactcatg gagggttcct gattcgtaga aaaagtgaat ggctgctatt ctatgttatt
     7441 cataacaagt taaatactcg tcaaacatca ggctgcttgt actggtgcaa tcctgatttc
     7501 ttagagtata aaagttttgt gcatttgaaa cattacgctt tgcaaaggat tttcatggaa
     7561 cgtgcgagta aaatgccgtc atcttatttg tatgaccaat aagtgatcat tggatgaaaa
     7621 ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct gcgacgcaga caactgattc
     7681 gccaacttct tgagcgcgat aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg
     7741 gcacgcttgt tgggctggca gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc
     7801 aacgtatggg ggcgctggta catactgctg ataattatcc gcttctgtta accgtcgctt
     7861 ttctctgttc ggcggtgctg gcgatgtttg gctacttttt ggtgcgcaaa tacgcgccgg
     7921 aagcaggtgg ttcggggatc ccggaaattg aaggggcgct ggaagatcaa cgtcccgttc
     7981 gctggtggcg tgtattgccg gtgaagttct ttggcgggct ggggacactc ggcggaggca
     8041 tggtgttggg gcgcgaaggg ccaaccgtgc agatcggcgg taacattggc cgtatggtgc
     8101 ttgatatttt ccgcctgaaa ggtgacgaag ctcgccatac gctgctggca accggtgctg
     8161 ctgcggggct ggctgcggcc tttaacgcgc cgctggcggg tattttgttt attatcgaag
     8221 agatgcgtcc gcagtttcgc tatacgttaa tttcgattaa agcggtattt attggtgtca
     8281 ttatgtcgac cattatgtac cggattttta atcatgaagt tgcgttgatt gacgtcggta
     8341 aactttctga tgcgccgctt aatacgctgt ggctttatct gatcctcggt attatttttg
     8401 gcattttcgg ccctattttt aataaatggg tgctggggat gcaggatttg ctgcaccgtg
     8461 tgcacggcgg caatattacc aaatgggtgc taatgggcgg tgcgattggc ggtctgtgtg
     8521 gattgctggg gtttgtggca ccagcaacgt cgggcggcgg ttttaacctg attcctatcg
     8581 ctaccgcggg gaatttcagc atgggaatgc tggtgtttat cttcgtcgcg cgggtcatta
     8641 ccaccttact ctgcttctct tccggcgcgc cgggcggtat ttttgccccg atgctggcgc
     8701 tgggtactgt gctgggaacc gctttcggaa tggttgccgt tgagctgttt ccgcaatatc
     8761 accttgaggc ggggacgttt gctattgccg gaatgggggc attactggcg gcatctattc
     8821 gcgcgccgtt aacggggatc attctggttc tggagatgac cgataactac cagctcattt
     8881 tgccaatgat tattaccggt cttggcgcaa cactattagc gcaatttacc ggcgggaaac
     8941 cgctatactc ggcgattctt gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc
     9001 gaagcaaggc cgcatcagcc agcgagaata cttgaacgaa ataccagggt attagataat
     9061 ggcgattatt attgggttag aatttgccca attgccgatg tcgtttggag caaaatatga
     9121 gtgatgacgt agcactgccg ctggagttta ccgacgcagc agccaacaaa gttaaaagcc
     9181 tgatcgctga cgaagataac ccgaatctga aattacgcgt gtatatcacc ggtggcggtt
     9241 gcagcggctt ccagtatggt ttcacctttg atgatcaggt gaacgaaggc gatatgacca
     9301 tcgaaaaaca gggcgttggc ctggtggttg atccgatgag cctgcaatat ctggtcggcg
     9361 gttccgttga ttataccgaa ggtctggaag gttctcgttt catcgtgacc aacccgaacg
     9421 cgaaaagcac ctgcggttgc ggttcttcct ttagtatcta atctgttgtt catgtgccgg
     9481 ataaactatc cggcacatct tctctcttca acgcccattc tcatccagcg caaacgtcgg
     9541 tagcttaaga tgccaacgaa tagccgccag ccgaatcaat agcgtcacga ccatgcccat
     9601 catactggct gtttccagtg gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc
     9661 gccgataata caggcagttg cgtagatttc tgtacgtaaa atcatgggga tttcgcgggc
     9721 cagaacatca cgaatgatcc cgccgccaac gccagtaatg acgcccatac aaaccgcgat
     9781 taacggaccg gcttccgcat taaaggcttt attcacgcca atgccgacaa acaccgccag
     9841 accaacggcg tccagcaccg gcaacatcca ttttggtaag cgtcttggct ggcgcaccag
     9901 cacgatggtc agcatgctgg tgaccattgc aacgaccaga tcggtgggat ctttcaccca
     9961 aaataccggg ccgtgatcca gcgccatgtc gcgaattgtc ccgccgccta ctgcggttac
    10021 cacgcccagt accagaacac caaaagggtc catacgcaat tttccggcta acaaaacgcc
    10081 ggagatggca aataccgctg tgccgactat atccagccaa tagacgagca ttgttcaatc
    10141 cccactgagc acctgaaaag gtcaggcgct aatctacctg tgaaagcgca ttacagagct
    10201 gttgtgcagc gaggataata cgtgggcttg cacgttcaaa ccagtcactc gtgagaggaa
    10261 taacgggaat tttgagctgt tcaccccagt attgtttgat tttaggaatt tggtccggtc
    10321 cgcctgtaat gacaatcgcc tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg
    10381 gccagggaac ccggctgtct ttaaagatgt tttctccgcc acaaacttcg agtacctggt
    10441 tctgaatcga ctcttttcca ctggtaaatg gcggattaat gccgaattgc agaaaaacac
    10501 gttttttagg tttatcagca tattgcgctt tcaattgcgc gtactgatcc agcagggatt
    10561 gcgcggcttg ttcggccttg tctggttgcg gactccaggg ggccagttga cgtaacgcat
    10621 tggcaatttg ttcaatgctt gtcgcatcga cccacatcac ttttattccc agcgaagcca
    10681 gctggtcaac ctgccgctcg gcattacctc cacgccaggc aatcaccaga tcgggtttca
    10741 gcgcgacaat gcgttccaga ttcatcccct gccaggtgga aacctgctca atcttttgcg
    10801 cttgtggagg atagtcggaa tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg
    10861 caagttcagt gttggcggga gaaagcgtga tgacgcgcgg cgcggcgttg agccacagtg
    10921 gcgcaagaaa agacagggcg accagcgccc tgaacagtga cttagccatg tgcaagtttc
    10981 tgcaccagtg actcaaccat caggctggac tgtttagcgg caacagccag gaactcatcg
    11041 aagctaagat gagactgttg atcggccacg tcggagatgg cgcgtacgac aacaaacggg
    11101 acgttgaaat tgtggcagac atgggcgatt gccgtcgctt ccatctctac agcaatggcc
    11161 tgtgggaagt tgtggcggat tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg
    11221 ctaacaatca ggccacgtac agcgttaaga ttcagttcgg caatgcaggc ctcagcggca
    11281 gcgatcagtt tatcgtcagc tttaaagcct gccggacagc ctggtaactg accgtattca
    11341 taaccaaatg ccgtgacatc cgcgtcgtga taacgtgctt cgtccgagac aacgatatcg
    11401 cccactttca acgttggtgc caggccaccg gcagaaccgg tgttaataat cacatctggc
    11461 ttgcagtgtt ccaacagcaa agtggcaccc agcgccgcag cgactttacc gatgcccgat
    11521 ttcagaagcg caacctcggt tccattcagt tggccggtat agatttcgca accgccgaga
    11581 ctgatagttt gacggttttc gattttgtca cgcagcagcg taacttcttc ttccattgca
    11641 ccaatgatgc cgattttcat agatttactc gcgataagcc cgatttgaag gcatagttta
    11701 ccatgcgctt acggggaagc gtatttctca cgcgggagag gacatggcac agattgattt
    11761 ccgaaaaaaa ataaactggc atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca
    11821 tgagatcctg cggatcttcg agagcgatcg cgggcgtatc atcaactctc cggcaattcg
    11881 tcgtctgcaa caaaagaccc aggtttttcc actggagcgc aatgccgccg tgcgcacgcg
    11941 tcttacccac tcgatggaag tccagcaggt ggggcgctac atcgccaaag aaattttaag
    12001 ccgtctgaaa gagcttaaat tactggaagc atacggcctg gatgaactga ccggtccctt
    12061 tgaaagcatt gttgagatgt catgcctgat gcacgatatc ggcaatccgc cgtttggtca
    12121 ttttggcgaa gcggcgataa atgactggtt tcgccaacgt ttgcacccgg aagatgccga
    12181 aagccagcct ctgactgacg atcgctgcag cgtggcggca ctacgtttac gggacgggga
    12241 agaaccgctt aacgagctgc ggcgcaagat tcgtcaggac ttatgtcatt ttgaggggaa
    12301 tgcacaaggc attcgcctgg tgcatacatt gatgcggatg aatctcacct gggcacaggt
    12361 tggcggtatt ttaaaatata cccgtccggc gtggtggcgt ggcgaaacgc ctgagacaca
    12421 tcactattta atgaaaaagc cgggttatta tctttctgaa gaagcctata ttgcccggtt
    12481 gcgtaaagaa cttaatttgg cgctttacag tcgttttcca ttaacgtgga ttatggaagc
    12541 tgccgacgac atctcctatt gtgtggcaga ccttgaagat gcggtagaga aaagaatatt
    12601 taccgttgag cagctttatc atcatttgca cgaagcgtgg ggccagcatg agaaaggttc
    12661 gctcttttcg ctggtggttg aaaatgcctg ggaaaaatca cgctcaaata gtttaagccg
    12721 cagtacggaa gatcagtttt ttatgtattt acgggtaaac accctaaata aactggtacc
    12781 ctacgcggca caacgattta ttgataatct gcctgcgatt ttcgccggaa cgtttaatca
    12841 tgcattattg gaagatgcca gcgaatgcag cgatcttctt aaactatata aaaatgtcgc
    12901 tgtaaaacat gtgtttatcc atccagatgt cgagcagctt gaattgcagg gctatcgggt
    12961 cattagcgga ttattagaga tttatcgtcc tttattaagc ctgtcgttat ccgactttac
    13021 tgaactggta gaaaaagaac gggtgaaacg tttccctatt gaatcgcgct tattccacaa
    13081 actctcggcg cgccatcggc tggcctatgt tgaggctgtc agtaaattac cgtcagattc
    13141 tcctgagttt ccgctatggg aatattatta ccgttgccgc ctgctgcagg attatatcag
    13201 cggtatgacc gacctctatg cgtgggatga ataccgacgt ctgatggccg tagaacaata
    13261 accaggcttt tgtaaagacg aacaataaat ttttaccttt tgcagaaact ttagttcgga
    13321 acttcgcgct ataaaacgaa tctgaagaac acagcaattt tgcgttatct gttaatcgag
    13381 actgaaatac atgaaaaaaa ccacattagc actgagtgca ctggctctga gtttaggttt
    13441 ggcgttatct ccgctctctg caacggcggc tgagacttct tcagcaacga cagcccagca
    13501 gatgccaagc cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt
    13561 agaaggtagc acaaccgtta atacgccgcg tatgccgcgt aatttccagc agttcttcgg
    13621 tgatgattct ccgttctgcc aggaaggttc tccgttccag agctctccgt tctgccaggg
    13681 gggccagggc ggtaatggcg gcggccagca acaaaaattc atggcgctgg gttccggcgt
    13741 catcattgat gccgataaag gctatgtcgt caccaacaac cacgttgttg ataacgcgac
    13801 ggtgattaaa gtccagctga gcgatggtcg taagttcgac gcgaagatgg ttggcaaaga
    13861 tccgcgctct gatatcgcgc tgatccaaat ccagaatccg aaaaacctga ccgcaattaa
    13921 gatggcggat tctgatgcac tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt
    13981 tggtctgggc gagacggtaa cttccgggat tgtctctgcg ctggggcgta gcggcctgaa
    14041 tgctgaaaac tacgaaaact tcatccagac cgatgcagcg atcaaccgtg gtaactccgg
    14101 tggtgcgctg gttaacctga acggcgaact gatcggtatc aacaccgcga tcctcgcacc
    14161 ggacggcggc aacatcggta tcggttttgc tatcccgagt aacatggtga aaaacctgac
    14221 ctcgcagatg gtggaatacg gccaggtgaa acgcggtgag ctgggtatta tggggactga
    14281 gctgaattcc gaactggcga aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag
    14341 ccaggttctg cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac
    14401 ctcactgaac ggtaagccaa tcagcagctt tgccgcactg cgtgctcagg tggggactat
    14461 gccggtgggc agcaaactga ccctgggctt gctgcgcgac ggtaagcagg tcaacgtgaa
    14521 tctggaactg cagcagagca gccagaatca ggttgattcc agctccatct tcaacggcat
    14581 tgaaggtgct gagatgagca acaaaggcaa agatcagggc gtggtagtga acaacgtgaa
    14641 aacgggcact ccggctgcgc agatcggcct gaagaaaggt gatgtgatta ttggcgcgaa
    14701 ccagcaggca gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt
    14761 gctggcactg aacattcagc gcggcgacag caccatctac ctgttaatgc agtaatctcc
    14821 ctcaacccct tcctgaaaac gggaaggggt tctccttaca atctgtgaac ttcaccacaa
    14881 ctccatacat cttcatcatc ctttaggcat ttgcacaatg ccgtacgtta cgtacttcct
    14941 tatgctaagc cgtgcataac ggaggactta tggctggctg gcatcttgat accaaaatgg
    15001 cgcaggatat cgtggcacgt accatgcgca tcatcgatac caatatcaac gtaatggatg
    15061 cccgtgggcg aattatcggc agcggcgatc gtgagcgtat tggtgaattg cacgaaggtg
    15121 cattgctggt actttcacag ggacgagtcg tcgatatcga tgacgcggta gcacgtcatc
    15181 tgcacggtgt gcggcagggg attaatctac cgttacggct ggaaggtgaa attgtcggcg
    15241 taattggcct gacaggtgaa ccagagaatc tgcgtaaata tggcgaactg gtctgcatga
    15301 cggctgaaat gatgctggaa cagtcgcggt tgatgcactt gttggcgcag gatagccgtt
    15361 tgcgggaaga actggtgatg aacctgattc aggcagagga gaatactccc gcacttactg
    15421 aatgggcgca acggctgggg atcgatctca atcaaccgcg agtggtggct attgttgagg
    15481 tcgacagcgg tcagcttggt gtggacagcg caatggcgga gttacaacaa ctgcaaaacg
    15541 cgctgactac gcccgagcgt aataatctgg tggcaattgt ctcgctgacc gaaatggtgg
    15601 tgttgaaacc ggcgttgaac tcttttgggc gctgggatgc agaagatcat cgtaagcgag
    15661 ttgaacaact gattacccgc atgaaagagt acggccagct gcgttttcgc gtttcactgg
    15721 gcaactattt taccggtcct ggcagtattg cccgatccta tcgtacggcg aaaacgacga
    15781 tggtggtggg taaacagcgg atgccagaaa gtcgctgcta tttttatcag gatctgatgt
    15841 tacctgtgtt actcgacagt ttgcgtggcg actggcaggc caacgaactg gcgcgaccgc
    15901 tggcgcggct gaaagcgatg gacaataacg gcttgctgcg acgaacgctg gcggcgtggt
    15961 ttcgtcacaa tgtgcaaccg ctggcaacgt caaaggcgtt gtttattcat cgtaataccc
    16021 tggagtatcg gcttaatcgt atatcggaac tgaccgggct tgatttgggc aattttgatg
    16081 acaggttgct gctgtatgtg gcgttacaac tggatgaaga gcggtaggtt atgcgttaag
    16141 gttgaggcgg cgctggctca ttcccctcag cccgaagggg agaggggaat gcccgagtta
    16201 accgtcaatt cgggattatt tacgcgttag tttttccaga tcggcttcaa tctcgctgat
    16261 cttattggtt acgactgact ccaggtgacg taagtcgtcg aggatcttac gcttaagatc
    16321 aacttcgctg cggtcacgct ggcagatttg atcaagctca tcaatgatat accgtagatt
    16381 cgggctgatt tcctggactt ctttataacc ctgacccaca ccatcagcga cgaccgtttt
    16441 acgctgacgc ggatatttaa acttaacgct cttggcgaaa aactcgcctt tgtctttctg
    16501 gaaatagatt ttcagaatat cgttgttggc ttcctgccgg aggctgtaac gatcaatttc
    16561 ttcaggattg gtaataccca gacttttcag attgtcgtac atagcgttac ctcaaaatga
    16621 gtcagtaaat tgtgcttatt ttagcatttg gcctggcccg cccggctcga ctgtgatcgc
    16681 agaaagcctt tttcttccag atttctatca tggcgtaaaa atcaaaaaat tacctgcttt
    16741 attgtggtga taaaattcac gttctacacc agttccagca tcgttacttc cgggcggcag
    16801 ttcagacgca aaccatacaa actgcccacg ccacgggttg tgtagatgtg tcgttcgcca
    16861 aaggcattta atccggcgac gtaacgttta tcttcgacag gggcaaaagg ttcaccaacc
    16921 aacggtacgc gcagttgccc gccgtgagta tggccgcaca gcatcagatc ccagggttca
    16981 tcacgcatga cttctttgct gtcgggatta tgtgccagca tcagtcttgg cagatttgct
    17041 tcgctggcgg gaggtggttt gcattgtccg gcccataagt caccggtgcc taccagttcg
    17101 aattgcctgt tcggcgtggc gatcaccgta gcctggttaa acaacaccgt gatgcccact
    17161 gatttcaacg tctcgccaat taagtgattt ttttctgtac caacagggcg atcgtggttg
    17221 ccgaagcagg caaacgtcgg cgcacattcg gcaaggggag agagtacgtt actaaacgcc
    17281 gaaaaattca gcggcatatc aaataatacg tagtcgccgc ccagcaatat caaatcgggc
    17341 ttttgttcta tgccaagagc aatcgcgtca gaaattaggc ttaaaggaac aaaacgagag
    17401 taatggagat ctgccagaaa aagaattttg aagggtgcgg ctttgtcttt aaaaaaggcg
    17461 atgcgctgat gggttaattc gagccaacct ggttcccaat aacgcatata gccgaagcct
    17521 gatccggcag ttaacgttgc ggcggcagcc agtaacaaac atctgcgtga aatcatcgct
    17581 catccctgca aaaaaatcgg gcagcgtcgt gctgcccgtg tgcatacttt tagtcgatgg
    17641 tacgcagcag ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga
    17701 taaccgcaca gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca
    17761 cagaccccgc cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg
    17821 tgctctgacc gatgtatacg cccatggaaa taacggaacc ttcttcgaca atcacgccct
    17881 caacaacttc tgaacgcgcg ccgatgaagc agttatcttc aataatggtc gggttagcct
    17941 gcagcggctc cagcacgccg ccgatgccta caccgccaga cagatggacg tttttaccaa
    18001 tttgtgcaca ggaaccgaca gtggcccagg tatcaaccat cgtgccttca tcaacatatg
    18061 cgccgatgtt gacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac
    18121 gtaccgccgc tggtggtaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt
    18181 cggcgaattt catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat
    18241 tatcattaat acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact
    18301 gaccgtcaat tttttccgcc acacgcagtg cgccggaatc cagcagggcg atcacctgat
    18361 ttaccgcttc gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa
    18421 aagcggtctc aataatgttc tgtaactgct gcattgttaa actctttcca tataaataaa
    18481 cacattaccc tttatcgttt ggattgaggg cctctgtcaa ccgctgatgt acttcctgct
    18541 gcaactcgtt attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc
    18601 gttcgccaat ggttgtaatt cgggcgccat gaagcgaaat tcccagatcg gcaaaaattt
    18661 tcccgactct cgccagcagt cctggctggt cgagcgcgat aagctcaagg aatgatttgc
    18721 ggtcggtatg ggttggcaaa aacgtcactt cggtttcaac agtaaaatgg cgcaatttgg
    18781 caggttggcg acgtggctgc ggtggctgcc agctactttg tgtcagtacc tgctccagac
    18841 caaatcgaat aacttcatgg cgatctgcgg aaagtgggct gccatcgggt tccagcacga
    18901 taaaggtatc catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta
    18961 aattgcggcg gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc
    19021 agataaaaat ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc
    19081 ttaaatcatg ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga
    19141 caaaatagtt agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat
    19201 ccatgcgcag tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatctg
    19261 gcgtgttttg cattccgcgt cgtagctgtt tttcggtggc aaaatagagt tcgcgcaaca
    19321 gactttgctt ccagctattc cacagcgttt cgttggtggc gcaaatgtca gccaccgtca
    19381 ggcataccag ataacgaagt cggttttctg tttgcacttc ttcggcaaac tgcttgatga
    19441 cttccgggtc ctgaatatcg cgacgctgag ctgtcaccga catcagcaag tgctggcgaa
    19501 ccagccaggc gaccagctgt gtttcgcgtg agttcagccc gtggagttcg gcaaaatgca
    19561 ctacatcctg agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa
    19621 acagcgcggc gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca
    19681 acggatggcg ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga
    19741 tagtatgttc atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat
    19801 gcgaccattg cggcatatac gcgccgagca cgctatggcg atgcattggc aatagcccgc
    19861 gccgcaccgc tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tccggaatat
    19921 tacacagcgg ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt
    19981 aaatgccggt gatcgcactg ttgtgcacca tggtgtagaa catacgcaag atggcttccg
    20041 gctggcgcat aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact
    20101 catcgtcgat tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca
    20161 gttgcagcag catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca
    20221 tcatccgctc gaccggttcg ttaccttcac cactgtaatt cagacgctgg gcgacgctaa
    20281 gctggcgatc gaataacagg cgattatcgt aacggctgac gaccagatgc agggcaaagc
    20341 gaatacgcca caatatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga
    20401 agccaaaccc gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt
    20461 gcagagtgtg gatatcgcgc aagccgccag ggctgctttt gatgtctggt tcaaggttgt
    20521 agctggtgcc atggtaacgc tgatggcgct ggttctgttc ttcaactttc gccgcgtaga
    20581 acttgtcgga aggccagaat ccttcgctga aaatatgttt ttgcagttcg aggaacagcg
    20641 caacatcgcc aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata
    20701 acccttccag catgcactct tcaagcgtgc gcacgctatg accgacttcc agctttacat
    20761 cccagagcag cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac
    20821 ggcttaaaat cagtaaatcg acgtctgaaa gtggatgcag ctcgccacga ccgtagccac
    20881 cgacggcgac caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt
    20941 gcaggagctg gtcgataaac tcggtgcgcg cctcaatcaa ctgttctgca gagatcccat
    21001 tgtcaaaggc atcacccagc caacgctgga aagtatcgat atgggctttt atcccaccga
    21061 cggttaattc atcacggggc cagacgcatg gattttgcgg ttgaccgggc agggtgggga
    21121 gagcggtgtt tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa
    21181 ttatcgcatt aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc
    21241 gccgggatgg tgtcatcctt gcgtagcgtc agaatttcgc agccgttatc agtcaccaca
    21301 atagtatgct catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct
    21361 ttcatggtgc ggatctcttt tttacccgcg ttgaccattg gctcgatggt gaacgtcatc
    21421 ccaggtttca gtacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca
    21481 tggaagccgc gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct
    21541 tcgacaaatt tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg
    21601 cgtagcgcca ggtacaggct ttcttgcgtg atgcggcaca gacgttcgcc catgatggtc
    21661 ggcttaccga cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg
    21721 acatcaatgt taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg
    21781 cacaccactt cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag
    21841 gcagaaaccg cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg
    21901 gtgctgacgc ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg
    21961 ccagcgacgc gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct
    22021 gtccatcagc gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc
    22081 cgggcaaatt gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat
    22141 agaaaattcg acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat
    22201 ttgtggtata aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc
    22261 gacaatctca ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg
    22321 gggtcggtaa tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc
    22381 atggcaactg tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc
    22441 cgttactgga acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc
    22501 atcaaccttg agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt
    22561 gcttctcgca aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg
    22621 aaagacgctg ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg
    22681 ctgactaact ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag
    22741 tctcaggacg gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag
    22801 ctggagaaac tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct
    22861 ctgtttgtaa tcgatgctga ccacgaacac attgctatca aagaagcaaa caacctgggt
    22921 attccggtat ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc
    22981 ccgggtaacg acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc
    23041 gtacgtgaag gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct
    23101 gagtaataag gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc
    23161 gagaattccc gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat
    23221 ctgcggcgcg aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta
    23281 gggggcctgc atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag
    23341 atcacatctc cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc
    23401 gtgagcgtac tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg
    23461 acatcgagct ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag
    23521 caggcaacgt tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca
    23581 ttctggaagt taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg
    23641 cagacaaagt tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag
    23701 cacagttcga agaagaacgt gttgcgctgg tagcgaaaat tggtgagaac atcaacattc
    23761 gccgcgttgc tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg
    23821 gcgttctggt tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg
    23881 ttgctgcaag caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa
    23941 aagaatacca ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag
    24001 agaaaatggt tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc
    24061 cgttcgttat ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag
    24121 tgactggctt catccgcttc gaagtgggtg aaggcatcga gaaagttgag actgactttg
    24181 cagcagaagt tgctgcgatg tccaagcagt cttaattatc gaaaaggagc cgcctgaggg
    24241 cggcttcttt ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa
    24301 gcgacgtaca atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa
    24361 catggctacc aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc
    24421 tctgcagggc actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga
    24481 aatcaaagaa ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct
    24541 gttccgtggc gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg
    24601 gatgctggcg accgtaatga acggcctggc aatgcgtgat gcactgcacc gcgcctatgt
    24661 gaacgctcgt ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc
    24721 agaagctatc agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa
    24781 cccgttcttt accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt
    24841 ggtgctgaaa gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac
    24901 cgcaaccatg tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat
    24961 ggacctggcg gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat
    25021 gaacaaaccg ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac
    25081 ggaataattc ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt
    25141 aagtctggtt ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac
    25201 cggagcgtac aaaagtacgt gaggatggcg agcactgccc ggggccaaaa tggcaaataa
    25261 aatagcctaa taatccagac gattacccgt aatatgttta atcagggcta tacttagcac
    25321 acttccactg tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt
    25381 gattagcgat atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa
    25441 aacccaaatc agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt
    25501 cgtggaatat tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga
    25561 ttcccgtaca ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc
    25621 gattatggcg tccgatcttg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc
    25681 gctgccgccg ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc
    25741 agaacaagcg cgtgttgcag tacgtaacgt gcgtcgtgac gcgaacgaca aagtgaaagc
    25801 actgttgaaa gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca
    25861 gaaactgact gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga
    25921 actgatgcag ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc
    25981 ggctggcggc gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca
    26041 ccattctggg ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc
    26101 ccgaacactt ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac
    26161 agtgcctgga attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc
    26221 ttaaaacgat gctacagcaa cagggtagcc gcaccgaagt cttaagtggg caacaagccg
    26281 cttgcgatat ggcagcgctt gaggatgttg atcaggtgat ggcagccatt gttggcgctg
    26341 ctgggctgtt acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata
    26401 aagaatcact ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc
    26461 aattgttacc ggtcgatagc gaacataacg ccatttttca gagtttaccg caacctatcc
    26521 agcataatct gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg
    26581 ggtctggtgg ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgccggatc
    26641 aagcctgccg tcatccgaac tggtcgatgg ggcgtaaaat ttctgtcgat tcggctacca
    26701 tgatgaacaa aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc
    26761 agatggaagt gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg
    26821 gcagtgttct ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg
    26881 catggccgaa tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt
    26941 tgacatttgc cgcaccggat tatgatcgtt atccatgcct gaaactggcg atggaggcgt
    27001 tcgcacaagg ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg
    27061 cttttcttgc gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg
    27121 aaaaaatgga tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg
    27181 cgcgtgaagt cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca
    27241 gagagtcgcg ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt
    27301 aagtagttgg ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag
    27361 gcttcagtat tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc
    27421 tgctactcaa ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat
    27481 tatggacggc aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa
    27541 agccggggca aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc
    27601 gttaacgctg tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc
    27661 gttaatggaa ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa
    27721 cgtgcgtctg cgtattattg gcgataccag tcgctttaac tcgcgtttgc aagaacgtat
    27781 tcgtaaatct gaagcgctaa cagccgggaa taccggtctg acgctgaata ttgcggcgaa
    27841 ctacggtgga cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca
    27901 aggaaacctg caaccagatc agatagatga agagatgcta aaccagcatg tctgtatgca
    27961 tgaactggcc cctgtagatt tagtaattag gactgggggg gagcatcgca ttagtaactt
    28021 tttgctttgg caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt
    28081 cgatgaacaa gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg
    28141 cggcaccgag cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct
    28201 gatatctgct tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt
    28261 ggggttcgcc attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct
    28321 tagcggtttt accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt
    28381 ggcgctgatg ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga
    28441 aatctcactt tgggcttcgc tgggttggtg gattgtcgcg ctattgctgg tgctgtttta
    28501 cccaggttcc gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct
    28561 aaccattgtt cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa
    28621 tcattacagt ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc
    28681 cggcgcatat atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc
    28741 gggtaaaacc tggcaaggct ttatcggtgg actcgctact gcagcggtaa tctcatgggg
    28801 ttatggcatg tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt
    28861 cgcagcgtta gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg
    28921 aattaaggac agcggtcatt taattccagg acacggtggt attttagatc gtattgatag
    28981 cctgacggct gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta
    29041 acggaaggta atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt
    29101 gtacttatca ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc
    29161 gttgagcgtt tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc
    29221 accgaatatg ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc
    29281 gcagaaccgg tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa
    29341 cgagcggcga ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac
    29401 tggctggttt ttattattgg tgtgcctagc gtacgtccgg tggttggcga aatagcagcc
    29461 aattcgatag ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt
    29521 atcgaaacgc ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa
    29581 agcaccacca ttacagtagc gccatttggc agcgaccaac ggcgggatgt aaagctcgat
    29641 ttacgtcact gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt
    29701 cctcgtgggc cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc
    29761 aaggcaggtt tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag
    29821 tgggtgacct ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc
    29881 gaaaggcagg ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt
    29941 aaagcgattg gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa
    30001 gttgtacgcc agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag
    30061 ctgatgaagc tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac
    30121 aacctcagtg ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggtt
    30181 gtttattacc tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt
    30241 ccgttgcccg tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc
    30301 ggaccggtat ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg
    30361 ctgttaatgg ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag
    30421 aacgcataat aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg
    30481 ccaccgtata cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc
    30541 gtgtcgccgt tggtgcggcc ctcctcagta tgccggtgcg cacaggcgac acggttaatg
    30601 atgaagatat cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc
    30661 gcgtccttcg tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca
    30721 gcattacttt ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg
    30781 cttctggtgt gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag
    30841 gtctggaaga cttctactac agcgtcggta aatatagcgc cagcgtaaaa gctgtcgtga
    30901 ccccgctgcc gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg
    30961 aaatccagca aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc
    31021 atttccaact gcgtgacgaa gtgccgtggt ggaacgtggt aggcgatcgt aaataccaga
    31081 aacagaaact ggcgggcgac cttgaaaccc tgcgcagcta ctatctggat cgcggttatg
    31141 cccgtttcaa catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg
    31201 tcacggtgaa catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca
    31261 accttgccgg gcactccgct gaaattgagc agctgactaa gatcgagccg ggtgagctgt
    31321 ataacggcac caaagtgacc aagatggaag atgacatcaa aaagcttctc ggtcgctatg
    31381 gttatgccta tccgcgcgta cagtcgatgc ccgaaattaa cgatgccgac aaaaccgtta
    31441 aattacgtgt gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag
    31501 gtaacgatac ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat
    31561 ggctggggag cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg
    31621 aaactgtcga taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtcgtct
    31681 acaaggtaaa agagcgcaac accggtagct tcaactttgg tattggttac ggtactgaaa
    31741 gtggcgtgag cttccaggct ggtgtgcagc aggataactg gttaggtaca ggttatgctg
    31801 ttggtatcaa cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc
    31861 cgtacttcac cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg
    31921 cagatgacgc cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg
    31981 gcttcccgat taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc
    32041 tgtccaacat gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc
    32101 cgagcacctc tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt
    32161 ggacctataa caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga
    32221 ccggtaaagt gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg
    32281 cgacttatgt gccgatcgat gacgatcaca aatgggttgt tctggggcgt acccgctggg
    32341 gttatggtga tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg
    32401 gttccagcac cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc
    32461 cgcatcaggc cagtaattat gatccggact atgattacga atgtgcgact caggacggcg
    32521 cgaaagacct gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc
    32581 tcgagttcat caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt
    32641 ccttcttctg ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg
    32701 gatatccgga ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat
    32761 ggatgtcccc attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg
    32821 gagacaaggc agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa
    32881 aggaatgtag tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac
    32941 gcaaagaact gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg
    33001 ttattagctg caggtctcgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt
    33061 gcaatcgtca acatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac
    33121 acgctggaaa atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg
    33181 caggctaaaa tgaaaaagct gcagtccatg aaagcgggca gcgatcgcac taagctggaa
    33241 aaagacgtga tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat
    33301 cgcgcacgtc gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg
    33361 aaatccgttg ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac
    33421 aacagcagcg atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg
    33481 ccttcaattc gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc
    33541 gatatcgtca tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc
    33601 atggttaacc caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg
    33661 acccaggacg atcttccttt cgcgaaaagt gccgcactgg tagtgaagaa tccctacctg
    33721 acttacgcgc gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca
    33781 cccagtgcgg tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac
    33841 gcggtgattg agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc
    33901 gtaggtaaaa acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac
    33961 catgagatcc agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac
    34021 ggctttggtt atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta
    34081 attattggcg atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat
    34141 gacactatta ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc
    34201 gtgattggcg acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt
    34261 ggtcgttact gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac
    34321 aaagtgacgg ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat
    34381 tcctcaggca ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg
    34441 aacattgatg acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac
    34501 taacgttcca tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca
    34561 ggccgtgtta ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca
    34621 ctcatactct gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac
    34681 tggtggatcg cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct
    34741 ctgtcaatga gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc
    34801 tgattctgga agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac
    34861 tggaaccggg tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg
    34921 tcgtgcctgg cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga
    34981 cccgttttaa aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt
    35041 gtgctcgtag ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg
    35101 ccattgtgga agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg
    35161 ttggacccca tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg
    35221 gtcatactaa aattggccgc gataatgaga tttatcagtt cgcctccatc ggcgaagtta
    35281 accaggatct gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca
    35341 ttcgcgaaag cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg
    35401 gcagcgacaa cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc
    35461 gctgtattct cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga
    35521 tcatcggcgg catgaccgca gtccatcagt tctgcatcat tggtgcgcac gtgatggttg
    35581 gcggctgctc cggtgtggcg caggacgtcc ctccttatgt cattgcgcag ggtaaccacg
    35641 caacgccgtt cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga
    35701 ttaccgctat ccgcaatgcg tataagctga tttatcgtag cggtaaaacg ctcgatgaag
    35761 tgaaaccgga aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt
    35821 tctttgcacg ctcaacgcgc ggtctgattc gttaatgact gaacagcgtc cattaacgat
    35881 tgccctggtc gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct
    35941 gaaagaacat gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga
    36001 aggctgcgaa gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct
    36061 cggtcgtctg cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact
    36121 gaagccagat gtttttgttg gtattgatgc gcctgacttc aatattactc ttgaaggtaa
    36181 cctcaaaaag cagggtatca aaaccattca ttacgtcagt ccgtcagtct gggcgtggcg
    36241 acagaaacgt gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt
    36301 cgaaaaagcg ttttatgaca aatacaacgt accgtgccgc tttatcggtc ataccatggc
    36361 tgatgccatg ccattagatc cagataaaaa tgccgcccgt gatgtgctgg ggatccctca
    36421 cgatgcccac tgcctggcgt tgctaccggg gagccgtggt gcagaagttg aaatgcttag
    36481 tgccgatttc ctgaaaacgg cccagctttt gtgccagaca tatccggatc tcgaaatcgt
    36541 ggtgccactg gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc
    36601 gccagacctt tcagttcatt tgctggatgg gatgggccgt gaggcgatgg tcgccagcga
    36661 tgcggcgcta ctggcgtcgg gtacggcagc cctggagtgt atgctggcga aatgcccgat
    36721 ggtggtggga tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac
    36781 tgattatgtc tcgctgccaa atctgctggc gggcagagag ttagtcaaag aattattgca
    36841 ggaagagtgt gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa
    36901 aaccagccac gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc
    36961 cgatgagcag gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg
    37021 cacacgcagc tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc
    37081 gtcaccgctg cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa
    37141 aagctgagcg aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc
    37201 tggagtctgg gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc
    37261 atgctggcga tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt
    37321 gatggtaacc gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggcgatagc
    37381 cgcgtaccgg aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg
    37441 gcggcgctgg atattgtttt cccgcaatat ggttttgccc aacacaaagg gtacccaacc
    37501 gcttttcatc tggaaaaact ggctgaacac ggcgcgaccg aacaccatcg gcgcagcttt
    37561 gggcctgtca aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac
    37621 cggaatctga agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg
    37681 atgatcgatg gcctggccaa aaccgcaccg ttggtaaaaa aggcggcggc gttgggtatg
    37741 ccagcactgg cgatcaccga tttcaccaac ctttgtggtc tggtgaagtt ctacggagcg
    37801 ggacatggcg cagggattaa gcctatcgtc ggggcagatt ttaacgtcca gtgcgacctg
    37861 ctgggtgatg agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat
    37921 ctgacgttgc tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc
    37981 gatcgcgact ggcttatcga attaaacgaa gggttgatcc ttctttccgg cggacgcatg
    38041 ggcgacgtcg gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg
    38101 ttttatgaag aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg
    38161 gatgaagaaa gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc
    38221 gtggcgacca acgacgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc
    38281 gtcgcgatcc acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg
    38341 cagcaatata tgcgtagcga agaggagatg tgtgagctgt ttgccgacat ccccgaagcc
    38401 cttgccaaca ccgttgagat cgccaaacgc tgtaacgtaa ccgtgcgtct tggtgaatac
    38461 ttcctgccgc agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca
    38521 aaagagggcc tggaagagcg tctggccttt ttattccctg atgaggaaga acgtcttaag
    38581 cgccgcccgg aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc
    38641 ttcccgggct acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta
    38701 ccggtagggc caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc
    38761 accgacctcg atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt
    38821 gtctccatgc ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgag
    38881 cacgtagcgg acatgtacgg tcgtgatgcg gtatcgcaga tcatcacctt cggtacaatg
    38941 gcggcgaaag cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc
    39001 gatcgtatct cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa
    39061 gccgagccgc agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac
    39121 atggcgcgca aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt
    39181 atcgcgccga ccaaaattac cgattttgcg ccgctttact gcgatgaaga gggcaaacat
    39241 ccggtcaccc agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc
    39301 cttggtttgc gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg
    39361 gcgaagaatg gcgagccgcc gctggatatc gctgcgatcc cgctggatga taagaaaagt
    39421 ttcgacatgc tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg
    39481 aaggacctga tcaagcgtct acaacctgac tgcttcgaag atatgatcgc cctagtggca
    39541 ctgttccgcc ccggtccgtt gcaatcaggg atggtggata actttatcga ccgtaaacat
    39601 ggtcgtgaag agatctccta tccggacgta cagtggcagc atgaaagcct gaaaccggta
    39661 ctggagccaa cctacggcat tatcctgtat caggaacagg tcatgcagat tgcgcaggtg
    39721 ctttctggtt ataccctcgg tggcgcggat atgctgcgtc gtgcgatggg taagaaaaag
    39781 ccggaagaga tggctaagca acgttctgta tttgctgaag gtgcagaaaa gaacggaatc
    39841 aacgctgaac tggcgatgaa aatcttcgac ctggtggaga aattcgctgg ttacggattt
    39901 aacaaatcgc actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg
    39961 cactatcctg cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag
    40021 gtggtgggtc tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata
    40081 aactccggtc tttaccattt ccacgtcaac gacgacggcg aaatcgtgta tggtattggc
    40141 gcgatcaaag gggtcggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc
    40201 ggctacttcc gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttgaaccgt
    40261 cgcgtgctgg aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acatcgcgca
    40321 gcgctgatga actcgctggg cgatgcgtta aaagcggcag atcaacacgc gaaagcggaa
    40381 gctatcggtc aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa
    40441 tcctacgcca gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg
    40501 ttaggcctgt acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat
    40561 gtcggaggcg taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct
    40621 gcggggctcg ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc
    40681 tgcacgctgg atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat
    40741 aaataccagc aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt
    40801 gatgacttca gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc
    40861 cgggaaaaat atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag
    40921 cttttaaacc gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat
    40981 ctctactatc agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc
    41041 tctccgagcg atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa
    41101 ctggagtttg actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg
    41161 attgcagagc tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa
    41221 ctggatatta acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgaca
    41281 cgtaaaatct tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccacag
    41341 cgtccttata ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc
    41401 gaccgcgcgt atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt
    41461 ccggtgatga tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac
    41521 tttggtatgc cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa
    41581 cgctttaaga tgccaatcat cacctttatc gacaccccgg gggcttatcc gggcgtgggc
    41641 gcagaagagc gcggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt
    41701 agcgtaccga ctatttgtac cgttatcggt gaaggtggtt ctggcggcgc gctggcgatt
    41761 ggcgtgggcg ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa
    41821 ggttgtgcgt ccattctgtg gaagagcgct gataaagcgc cgctggcggc tgaagcgatg
    41881 ggtatcatcg ctccgcgtct gaaagaacta aaactgatcg actccatcat cccggaaccg
    41941 ctgggtggtg ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgttg
    42001 gcggatctgg ccgatctcga cgtgttaagc actgaagatt taaaaaatcg tcgttatcag
    42061 cgcctgatga gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt
    42121 ggcccttttt tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg
    42181 aacacatgaa catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca
    42241 tcaaagaact ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca
    42301 gcgttgattt gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact
    42361 gggatgagta cagtctcgat ttatgtagcg atatcaatca gcttaatgaa tatctcccgc
    42421 tttatgcctt catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg
    42481 cgctctggtt ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc
    42541 agtacaccga cgaatatctt gataacatta caccgccgtt cacgaaagcc ttgtttacct
    42601 acgtcaaaga gcggaagtac accttttgta cgccggggca tatgggcggt accgcctatc
    42661 aaaaaagccc ggttggctgt ctgttttatg attttttcgg cgggaatacc ctcaaggctg
    42721 atgtctctat ttcggttacc gagcttggtt cgttgctcga ccacaccggg ccacacctgg
    42781 aagcggaaga gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg
    42841 gaacatcgac gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt
    42901 tgatcgaccg caattgtcat aaatcgctgg cgcatctgtt gatgatgaac gatgtagtgc
    42961 cagtctggct gaaaccgacg cgtaatgcgt tggggattct tggtgggatc ccgcgccgtg
    43021 aatttactcg cgacagcatc gaagagaaag tcgctgccac cacgcaggca caatggccgg
    43081 ttcatgcggt gattaccaac tccacctatg atggcttgct ctacaacacc gactggatca
    43141 aacagacgct ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tacacccatt
    43201 ttcatccgat ctaccagggg aaaagtggta tgagcggcga gcgtgttgcg ggaaaagtga
    43261 tcttcgaaac gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcgctgatcc
    43321 acattaaagg cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca
    43381 cctcgcccag ttatcccatt gttgcttcgg ttgagacggc ggcggcgatg ctgcgtggta
    43441 atccgggcaa acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg
    43501 tccagcggtt gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg
    43561 tggatgaagc cgaatgctgg cccgttgcgc ctggcgaaca gtggcacggc tttaacgatg
    43621 cggatgccga tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg
    43681 acgaacaggg caatatgagc gaggagggga tcccggcggc gctggtggca aaattcctcg
    43741 acgaacgtgg gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta
    43801 ttggtatcga taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaaacgct
    43861 cttacgatct caacctgcgg atcaaaaata tgctacccga tctctatgct gaagatcccg
    43921 atttctaccg caatatgcgt atccaggatc tggcgcaagg gatccataag ctgattcgta
    43981 aacacgatct tcccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga
    44041 cgccacatca ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac
    44101 aactggtcgg tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt
    44161 tgatgccagg agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc
    44221 tttgttccgt agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg
    44281 acgaagacgg cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg
    44341 cttccgggcg agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca
    44401 ggttcaccat attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga
    44461 tattcttggt ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg
    44521 ggatttggcg cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga
    44581 gcgccccagc cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat
    44641 cgatgcggca gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga
    44701 tccatacacg caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact
    44761 gtatgagcag taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac
    44821 tgtaaaatcg caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga
    44881 ttctggtggc ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt
    44941 ggcggacgga aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg
    45001 ccaatgccga tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc
    45061 tggtggtcga acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc
    45121 aggcgcgtta tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc
    45181 aacatctcga cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg
    45241 ccgggctttc ggctatggcg gaagtttcgg agtttgccgg aacacggctt atccgcccgt
    45301 tgcttgcccg cacgcggggg gaactggcgc agtgggcatt ggcgcatggt ttacgctgga
    45361 ttgaagacga aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag
    45421 tgccgttatt gcagcagcgt tggccgcatt ttgccgaagc aacggcccgc agcgccgcac
    45481 tttgtgctga acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc
    45541 aaacgtcgca ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgccgcg
    45601 cggcgattat ccgtcgctgg ctggcagggc agaatgcacc aatgccttcc cgcgacgcgc
    45661 tggtgcggat ctggcaggaa gtggcgctgg cgcgggaaga tgcctccccc tgtttacgct
    45721 tgggcgcgtt tgaaatccgc cgatatcagt cgcaactgtg gtggattaaa tccgtcaccg
    45781 ggcaaagtga aaccattgtg ccgtggcaga cgtggctaca accgctggaa ttaccggcgg
    45841 ggctgggaag cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag
    45901 cggtcagcgt gcgtttcaaa gcggcaggat tgctgcatat tgtcgggcgc aacggcggac
    45961 gtaagctgaa gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc
    46021 cactgctgtt ttatggcgaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag
    46081 gtgtggctga aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga
    46141 aagccggata agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ctcgctcacc
    46201 accaccgtac cgatttccgg gtggctaaag ctggtaattt tatccagacg tagctcacgg
    46261 gtttcgccag cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcgctg
    46321 gcttttgcct gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg
    46381 caggctagct caagattatc gtaatcatca caattgattg gttgatacgt atcattcatt
    46441 gacataatcg ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc
    46501 agaaagggcg tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc
    46561 cggaacgtat cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca
    46621 atatttttcc acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc
    46681 ctacgaagat aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag
    46741 cgccgcaact gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg
    46801 gcgcttaaag cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag
    46861 tttctcgatg cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg
    46921 ctgcgcttgc tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg
    46981 tgtgccgatg atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg
    47041 attgagctgg ggctgccgga tgagcggcgg attaagaaag cctgcaccca ggccgcagaa
    47101 gtggcgctgt ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc
    47161 aaatgtgtgc agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa
    47221 gtaagcgcct ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggcgtgatc
    47281 tggttatcgg acgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca
    47341 tgattgtgat ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc
    47401 gtgcgcaggg cgcgggcggg cagcatgtta ataagacctc aacggccatt catctgcgtt
    47461 ttgacattcg ggcgtccagc ctgccagagt attacaaaga gcgtctactc gccgccagcc
    47521 atcatttgat cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg
    47581 aactgaaccg cgaagccgcg ctagcccggc tggtggcagt gattaaagat ttaacaacag
    47641 aacaaaaagc ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat
    47701 cgaaagcaca aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat
    47761 aaaaagaagg aatagatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc
    47821 tttactctga tgggatgtaa taaccgggcc gaagtcgata cgctttctcc agcacaggct
    47881 gccgaactga aaccgatgcc gcaaagttgg cgcggcgtgc tgccgtgtgc tgattgcgaa
    47941 ggaatcgaaa cctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat
    48001 ctcggtgctc gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct
    48061 gacaagctgg tattaaccga cagcaaaggt gaaaagtcat attatcgggc caaaggcgat
    48121 gcgctggaga tgctcgatcg tgaaggcaat ccgattgaat cgcagttcaa ctatacgctg
    48181 gaaccggcac aatccagttt acccctgacg ccgatgaccc tgcggggcat gtatttttat
    48241 atggctgatg tggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat
    48301 aacgcagagc tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta
    48361 ctgtcagtag aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa
    48421 gtattagcgc ccgatacggc gggtaaattt tacctgaata aggattgcag tagtttgggg
    48481 cagtaaacct gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag
    48541 gcctacagca tataatgcaa cattttgaat taggtgctca acgcctgatg cgacgctggc
    48601 gcgtcttatc atgcctacaa cccccctcat accttaaggc ggataaggca tttactttat
    48661 caccgggtca aaccaacaaa gcgacccgcc ttgatataaa tcccaggctt caggtgccca
    48721 acatactgta actgcgtttc tggcttaaac gcagagacat caccaatacg catatgcatc
    48781 agatcggcag ggctaatcca gcccgattgc gccagtgtaa gtggatgccc ggctttggca
    48841 aaggcatccg tgacaaattc cgaacaaaac cacgactttt tgtctccttc gcccacactg
    48901 cttaactgcg ctttcgccag gccgctgacg cactgttggc gaaaatcctc ggagaacgga
    48961 ttcagtgagc acatctggcg agtcaccata aagggaataa attcgacaat gccgcgatag
    49021 ttataaccgc tatctttgat tttattggca aacgcggtga tatctgtggc ttgttgcggg
    49081 gtaagatccg ggactcgtaa gacgaaaagc ttatcactat gcttcatcgc ttttttaagg
    49141 gaaacgatct ggacgccagc gcctgtcgct tctgcaacgt tattatcacc cagaaagatt
    49201 gcaacgtgac tcacagagga agtgctgaag acgcggattc caaatgaggt taccccaagg
    49261 cttgaggaga acagcaaatc gccgggtttg agatcaggtg ccgttatttc ttttattgat
    49321 tgttcggtga aagagctttg atgctggaat ttgacagccc atgtttttgc ctcggcatct
    49381 actgctgtgg ctgatgggtc tggctggctg atatcaacgg tacaagccga aagtaaaaga
    49441 aagctgggga gaaacaggcg gcagtacgcc tttggtttat ccattttata caatccatgt
    49501 aaaaaaaggg ccctgaaatt caggaccctt tctggcatca gcctttaatc tgtttcacca
    49561 gatattcgac gatgtcacca gtcttaatta actgtttctc gccgttgcga cgatatttat
    49621 attcgatatc gtcgttgtcg aggttacggt cgcccagcac aatagtgtgc ggaataccga
    49681 tcagttccat atcagcaaac atcacgcccg gacgctcttt gcggtcatcc agcagcactt
    49741 cgataccttg tgcacgcagt tcgctgtaca gtttctcagc aagctcttgt acgcggaagg
    49801 atttgtgcat gttcatcggc agaatcgcca cctggaacgg cgcgatagcg tcaggccata
    49861 cgatgcctcg ttcgtcgtag ttctgctcaa tcgccgcagc taccacacgc gttaccccga
    49921 taccgtagca acccatcgtc aggatttggt tacggccatc ttcaccctgt acggaggctt
    49981 tcagtgcttc ggagtacttg gtacccagct ggaagatgtg accaacttcg ataccacgtt
    50041 tgatcagcag cgtaccctgg ccatccgggc ttggatcgcc agccaccacg ttacggatat
    50101 ctgcaacttc cggggtagcg acatcgcgat cccagttgat gccgaagtag tgtttaccat
    50161 cgatgttagc accagcagcg aaatcactca tcgccgcaac ggtacggtca atcaccaccg
    50221 gaatcggcat gtttaccgga cccagtgaac ccggaccggc tttaaccacg gcacgaattt
    50281 cttcttcggt cgcgaaagtc agcgggcttg caacctgcgg cagtttttct gctttaactt
    50341 cgttcagctc gtgatcaccg cgcaccagca gcgcaaccag cgggaagctg ctgccttcaa
    50401 ccgctttaac cagcagagtc ttaaccgttt tctcaatcgg cagattgaac tgttcaacca
    50461 gttccgcgat ggttttcgcg ttcggcgtat caaccagcgt catttcctgg gtagcagcag
    50521 cgcgcggttc tttcggcgcg atagcttctg ccagttcaat gttcgctgca tagtcagagg
    50581 tgtcggagaa gaccacatcg tcttcaccgc tctgcgccag cacctggaat tcgtgagagg
    50641 cgctgccgcc gatagaaccg gtgtcggctt gtacggcgcg gaaatccagc cccatgcggc
    50701 tgaagatttt gctgtaggcc gcatacattg catcgtaggt ttcctgcagg gattcctgag
    50761 aagtatggaa agagtaagca tctttcatca ggaattcgcg ggaacgcatg acgccgaaac
    50821 gcggacgcac ttcgtcgcgg aacttggtct ggatctgata gaagttcagc ggcagctgtt
    50881 tgtaagagct aagctcgtta cgaatcaggt cagtgataac ttcttcatga gttgggccga
    50941 gtacgaacgg acgctcgcca cggtcaacaa aacgcagcag ttccggaccg tactgttccc
    51001 aacgaccact ctcttgccac aaatcggctg gctgaaccac cggcatcgac acctcgatcg
    51061 caccggcgtt gttcatctct tcacgcacga tgttttcgac ttttttcaga acgcgcacgc
    51121 cggtcggcag ccaggtatat aacccggagg ccagcttgcg gatcatcccg gcgcgcagca
    51181 tcagctgatg gctgatcacc tcggcgtcgg caggtgtctc cttgagagtg gagagcaggt
    51241 attggctagt acgcatgttg ttacggttcc agttggaagg tagaacaggc tcaaggcgag
    51301 cctgggacaa aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg
    51361 cgtgaattta acgcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc
    51421 gaacgttaaa atcatgtagc cagacggcat aggttttgcc cgtttcctca cctttacgat
    51481 aggccgggcg cgggtcctgc gccagtactt cgcggataaa cagcgttaac tgcggataac
    51541 gcttctccag cgtcaaaagc tgcttttcga cctctgcggt aaaactcacc gccatctctg
    51601 cagctggcgc actttgcgca tagctggcac tggcatcggg aagcgattcg gcaaagggga
    51661 gatacggttt gatatccact accggcgtac catcgaccag atccagactg ccgagcttca
    51721 gaatcacgct gtctttatgg caaacaacct ctttcagctc taccagcgac atgccaattg
    51781 ggttagggcg gaaagtagag cgtgttgcga aaacccccat tctggcgtta ccgccgaggc
    51841 gcggtggacg cacagtcgga cgccagccgc cttccatcgt ttgatgaaag acgaaaagga
    51901 tccataaatg gctgaacgct tccaggccgc gaacggcgtc ggcctggttg tagggagcaa
    51961 tgagatgcag ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga acggcgaact
    52021 tttctttata gggcgagcga ataacgccta tttgctcgaa ctggaaactg ctcatttcgc
    52081 cgtaatgtta agcgcagaac cgatacatac agcctgacga tagcagcctg gcgtaccgct
    52141 ggtgacttcg cagctatgca gtaatacagc attggctttc attttagagg cgttgatttg
    52201 catccgctta cgtgcggttg gaatgctcgg cggagagtcc tgattagagg cctggcaaga
    52261 gtcgccactg acttcaccga gatcgcggaa cggtttgccg actaattctt ctgcattggt
    52321 ataaattcgg accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg
    52381 gggtgcagtg ctttgaacgg gttcgacagg ggatctgctt aacatggaac agccgcttag
    52441 catgagtgct actaaacaga tcggtaaagc acgcatagta tttcctcaat gtatgatcaa
    52501 aacgtcaata ttgaatcagg agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga
    52561 atgatattac aaattgtgga aacagcctaa aaattaccag cctttaacag ctccgccgtt
    52621 aaacactttg tttgctgctt cgtaaacttc gtcagactga taagcctgga cgaatttctt
    52681 cacgttctcg gcgtctttgt tatcttcacg cgtcacgatc aggtttacgt acggggactc
    52741 tttatcttca acaaagatac catctttcgc cggagtcagg ccaatctggc tggcataggt
    52801 ggtattgata actgccagag cgatttgcgc gtcgtccaga gaacgcggca gttgcggtgc
    52861 ttccagttca acaattttca gatttttggg gttctcaaca acatcaagaa cggtcggcag
    52921 caggccaacg ccatctttca gtttgatcaa gcccactttt tgcagcagca gcagtgaacg
    52981 accaaggtta gttgggtcgt ttggcacggc aacctgcgaa ccatcctgca gttcatccag
    53041 tgatttgatt ttcttggagt aaccagcaat cggataaaca aaagtgttgc ctactgcgac
    53101 cagtttgtag ccacgatctt tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt
    53161 ggcgtcgata tcgcctttgc tcaatgcttc gtttggcaga acatagtcgt tgaaggttac
    53221 cagctcaacg tccaggccat atttgtcttt cgcaactttc tgcgcgactt ctgcaacctg
    53281 ctgttcggca ccaacaatca cgccgacttt aatgtggttt ggatcttttt catcctgacc
    53341 gcagcctacc agtgccagtg atccaatcag ggctcccact gccgcaaagg ttttgaattt
    53401 gaacgccata ccttattcct tcttcaatta tttatgttgt gttgaacgtt acttgcgagt
    53461 gacagcccgg acgatgcggt cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa
    53521 taccagtacc gtattcatca ccgtcgcgtt gtagccgatg tagccatact gatagccaat
    53581 ctgacctaaa ccaccggcac cgactgcacc acccatcgcg gaataaccga ctagggtaat
    53641 cagggtgata gttgccgcat tcaccagacc cggcaacgct tccggtaaca gcactttacg
    53701 gacgatctgc attggcgtgg cacccattgc gcgggaagct tcaattaacc cggttgggat
    53761 ctccagaaga gcgttctcga ccatacgggc aataaacggc gctgcgccaa cggttaacgg
    53821 aacaatcgct gcctgcaaac caatcgatgt accgacaata actcgggtaa acggaatcat
    53881 ccataccagc aagataatga acgggatgga acggaaaatg ttcacaattg cagaaacggt
    53941 acgatacagc ttcgcgttag caataatttg ccccggacgc gtgacataaa gcagaacgcc
    54001 aaccggcaga ccaatcacaa agccaaaaaa accggatacg aaggtcattg ccagcgtttc
    54061 ccatacgcca cgaaccagca gccacatcat cggctcagac ataacccagt acctctactt
    54121 ttacatggtg ttcctgcagc caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca
    54181 tttcagtcag catgatgccg aacttaacgc caccggcgta gtccatctgc gcgctaataa
    54241 tgttgttgtt gacgttgaaa cgacgcgcgg tttcagaaag cagtggggca tcgaccgact
    54301 gaccggtaaa ctccagacgc agcatcggca cgcagtcagt gaatggctcc gcttgcagac
    54361 gttcctggta atcttccggg atatccagat gcagggtcga ctgaataaac ttctgcgcca
    54421 acggcgtttt cggatgcgaa aacacttcac ttaccgtgtc ctgctcgatc agttcgccac
    54481 tgctgatgac cgccacgcaa tcacaaatac gctttacaac gtccatttcg tgggtgatca
    54541 acagaatcgt caaacccaga cggcggttaa tgtctttcag cagctcgaga atagaacgtg
    54601 tcgttgccgg gtccagcgcg ctggtggctt catcacacag caatactttg ggattgctgg
    54661 ctaacgcacg ggcaatcgcc acacgctgtt tctgcccacc ggaaagattc gatgggtagc
    54721 tatcatgctt atcgccaaga ccaaccaatg acagcaattc cgtcacgcga cgtttgatct
    54781 cgtctttcgg tgtgttgtcc agctccagcg gcagagccac gttgccaaaa acagtacgcg
    54841 aagagagcag gttaaaatgc tggaaaatca taccaatctg gcggcgagct ttggtcaact
    54901 cggattctga cagcgtggtc agttcctggc catcgaccag cacgctaccc tcggttgggc
    54961 gctccagcag gtttacacaa cgtataagcg tactcttacc cgcgcctgag gcaccgataa
    55021 cgccataaat ttgtccagct ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc
    55081 gggtgccctg gtggaacact ttggtgatat tcgaaagttt tatcattgat tatttattat
    55141 cgtcattaag ttagtcgtgg catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat
    55201 ggatgttaag gcatccagac gtctaaatca atcaggttta tgcgaagagc actttcttgc
    55261 aggtcgaaac atgcgatact agcgtcacat gccttattaa ggagctataa aaggtggcaa
    55321 agagcgtacc cgcaattttt cttgaccgtg atggcaccat taatgtcgat cacggctatg
    55381 tccatgagat cgacaacttt gaatttatcg acggtgttat tgacgccatg cgcgagctaa
    55441 aaaaaatggg ctttgcgctg gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat
    55501 ttaccgaagc acagtttgaa acgctgaccg agtggatgga ctggtcgctg gcggaccgag
    55561 atgtcgatct ggatggtatc tattattgcc cgcatcatcc gcagggtagt gttgaagagt
    55621 ttcgccaggt ctgcgactgc cgtaaaccac atccggggat gcttttgtca gcacgcgatt
    55681 atttgcatat tgatatggcc gcttcttata tggtgggcga taaattagaa gatatgcagg
    55741 cagcggctgc ggcgaacgtg ggaacaaaag tgctggtgcg tacgggtaaa cctattacgc
    55801 ctgaagcaga aaacgcggcg gattgggtgt taaatagcct ggcagacctg ccgcaagcga
    55861 taaaaaagca gcaaaaaccg gcataatgat taaaagatga gcgattgaaa taaaaatgca
    55921 tttttccact tgtcttcctg agacgactcc ctataatgc
//
LOCUS       DAJOSY010000015        55599 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJOSY010000015 DAJOSY010000000
VERSION     DAJOSY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.015"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            119..586
                     /gene="slyB"
                     /locus_tag="OHN27_001018"
     CDS             119..586
                     /gene="slyB"
                     /locus_tag="OHN27_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCQ2285505.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(633..1067)
                     /gene="slyA"
                     /locus_tag="OHN27_001019"
     CDS             complement(633..1067)
                     /gene="slyA"
                     /locus_tag="OHN27_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCQ2285506.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            1268..1504
                     /locus_tag="OHN27_001020"
     CDS             1268..1504
                     /locus_tag="OHN27_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCQ2285507.1"
                     /translation="MKFILNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            1507..2364
                     /locus_tag="OHN27_001021"
     CDS             1507..2364
                     /locus_tag="OHN27_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2285508.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            2364..4376
                     /locus_tag="OHN27_001022"
     CDS             2364..4376
                     /locus_tag="OHN27_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCQ2285509.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(4377..4898)
                     /gene="sodC"
                     /locus_tag="OHN27_001023"
     CDS             complement(4377..4898)
                     /gene="sodC"
                     /locus_tag="OHN27_001023"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCQ2285510.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(4979..5875)
                     /locus_tag="OHN27_001024"
     CDS             complement(4979..5875)
                     /locus_tag="OHN27_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCQ2285511.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(5924..6163)
                     /locus_tag="OHN27_001025"
     CDS             complement(5924..6163)
                     /locus_tag="OHN27_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCQ2285512.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            6266..6865
                     /gene="nemR"
                     /locus_tag="OHN27_001026"
     CDS             6266..6865
                     /gene="nemR"
                     /locus_tag="OHN27_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCQ2285513.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            6902..7999
                     /gene="nemA"
                     /locus_tag="OHN27_001027"
     CDS             6902..7999
                     /gene="nemA"
                     /locus_tag="OHN27_001027"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCQ2285514.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            8080..8487
                     /gene="gloA"
                     /locus_tag="OHN27_001028"
     CDS             8080..8487
                     /gene="gloA"
                     /locus_tag="OHN27_001028"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCQ2285515.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            8590..9237
                     /gene="rnt"
                     /locus_tag="OHN27_001029"
     CDS             8590..9237
                     /gene="rnt"
                     /locus_tag="OHN27_001029"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCQ2285516.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            9330..13946
                     /locus_tag="OHN27_001030"
     CDS             9330..13946
                     /locus_tag="OHN27_001030"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCQ2285517.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQPQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGTTSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIEEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSVLLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(13997..14344)
                     /gene="grxD"
                     /locus_tag="OHN27_001031"
     CDS             complement(13997..14344)
                     /gene="grxD"
                     /locus_tag="OHN27_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCQ2285518.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            14678..15493
                     /gene="mepH"
                     /locus_tag="OHN27_001032"
     CDS             14678..15493
                     /gene="mepH"
                     /locus_tag="OHN27_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCQ2285519.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            15621..16202
                     /gene="sodB"
                     /locus_tag="OHN27_001033"
     CDS             15621..16202
                     /gene="sodB"
                     /locus_tag="OHN27_001033"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCQ2285520.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(16437..17606)
                     /locus_tag="OHN27_001034"
     CDS             complement(16437..17606)
                     /locus_tag="OHN27_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2285521.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(17772..17861)
                     /gene="ynhF"
                     /locus_tag="OHN27_001035"
     CDS             complement(17772..17861)
                     /gene="ynhF"
                     /locus_tag="OHN27_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCQ2285522.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            18160..19185
                     /gene="purR"
                     /locus_tag="OHN27_001036"
     CDS             18160..19185
                     /gene="purR"
                     /locus_tag="OHN27_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCQ2285523.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(19182..20114)
                     /locus_tag="OHN27_001037"
     CDS             complement(19182..20114)
                     /locus_tag="OHN27_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2285524.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            20227..21438
                     /locus_tag="OHN27_001038"
     CDS             20227..21438
                     /locus_tag="OHN27_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCQ2285525.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            21729..22877
                     /gene="cfa"
                     /locus_tag="OHN27_001039"
     CDS             21729..22877
                     /gene="cfa"
                     /locus_tag="OHN27_001039"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCQ2285526.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(22917..23558)
                     /gene="ribE"
                     /locus_tag="OHN27_001040"
     CDS             complement(22917..23558)
                     /gene="ribE"
                     /locus_tag="OHN27_001040"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCQ2285527.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            23773..25146
                     /gene="mdtK"
                     /locus_tag="OHN27_001041"
     CDS             23773..25146
                     /gene="mdtK"
                     /locus_tag="OHN27_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCQ2285528.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALAEKAVGYLRALLWGAPGYLFFQVARNLCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            complement(25187..26443)
                     /locus_tag="OHN27_001042"
     CDS             complement(25187..26443)
                     /locus_tag="OHN27_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCQ2285529.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGTGWLKVATGGIVKCTQYGNNGTLSVSDGAIATDVVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            26751..26827
                     /locus_tag="OHN27_001043"
     tRNA            26751..26827
                     /locus_tag="OHN27_001043"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26785..26787,aa:Val,seq:gac)
     gene            26832..26908
                     /locus_tag="OHN27_001044"
     tRNA            26832..26908
                     /locus_tag="OHN27_001044"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26866..26868,aa:Val,seq:gac)
     gene            27016..27321
                     /locus_tag="OHN27_001045"
     CDS             27016..27321
                     /locus_tag="OHN27_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCQ2285530.1"
                     /translation="MATLLQLHFSFNGPFGDTMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            27447..29051
                     /locus_tag="OHN27_001046"
     CDS             27447..29051
                     /locus_tag="OHN27_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCQ2285531.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFHGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(29063..29875)
                     /locus_tag="OHN27_001047"
     CDS             complement(29063..29875)
                     /locus_tag="OHN27_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285532.1"
                     /translation="MIITQADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALVYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(29879..30664)
                     /locus_tag="OHN27_001048"
     CDS             complement(29879..30664)
                     /locus_tag="OHN27_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCQ2285533.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQTHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(30661..31329)
                     /locus_tag="OHN27_001049"
     CDS             complement(30661..31329)
                     /locus_tag="OHN27_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCQ2285534.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(31393..32031)
                     /locus_tag="OHN27_001050"
     CDS             complement(31393..32031)
                     /locus_tag="OHN27_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCQ2285535.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYLVAEEELSVLLESIKQNGDYADIACLTGSKDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(32044..34146)
                     /locus_tag="OHN27_001051"
     CDS             complement(32044..34146)
                     /locus_tag="OHN27_001051"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCQ2285536.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(34167..34793)
                     /locus_tag="OHN27_001052"
     CDS             complement(34167..34793)
                     /locus_tag="OHN27_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCQ2285537.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(35248..35457)
                     /gene="fumD"
                     /locus_tag="OHN27_001053"
     CDS             complement(35248..35457)
                     /gene="fumD"
                     /locus_tag="OHN27_001053"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCQ2285538.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            36014..37426
                     /gene="pykF"
                     /locus_tag="OHN27_001054"
     CDS             36014..37426
                     /gene="pykF"
                     /locus_tag="OHN27_001054"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCQ2285539.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            37737..37973
                     /gene="lpp"
                     /locus_tag="OHN27_001055"
     CDS             37737..37973
                     /gene="lpp"
                     /locus_tag="OHN27_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCQ2285540.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(38037..39041)
                     /gene="ldtE"
                     /locus_tag="OHN27_001056"
     CDS             complement(38037..39041)
                     /gene="ldtE"
                     /locus_tag="OHN27_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCQ2285541.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRVTQ
                     "
     gene            complement(39190..39606)
                     /gene="sufE"
                     /locus_tag="OHN27_001057"
     CDS             complement(39190..39606)
                     /gene="sufE"
                     /locus_tag="OHN27_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCQ2285542.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(39619..40839)
                     /gene="sufS"
                     /locus_tag="OHN27_001058"
     CDS             complement(39619..40839)
                     /gene="sufS"
                     /locus_tag="OHN27_001058"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCQ2285543.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPGQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKQASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHLVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(40836..42107)
                     /gene="sufD"
                     /locus_tag="OHN27_001059"
     CDS             complement(40836..42107)
                     /gene="sufD"
                     /locus_tag="OHN27_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCQ2285544.1"
                     /translation="MAGLPNSSNALQQWHHLFEADGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGVR"
     gene            complement(42082..42828)
                     /gene="sufC"
                     /locus_tag="OHN27_001060"
     CDS             complement(42082..42828)
                     /gene="sufC"
                     /locus_tag="OHN27_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCQ2285545.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVHPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(42838..44325)
                     /gene="sufB"
                     /locus_tag="OHN27_001061"
     CDS             complement(42838..44325)
                     /gene="sufB"
                     /locus_tag="OHN27_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCQ2285546.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(44334..44702)
                     /gene="sufA"
                     /locus_tag="OHN27_001062"
     CDS             complement(44334..44702)
                     /gene="sufA"
                     /locus_tag="OHN27_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCQ2285547.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(45250..45438)
                     /locus_tag="OHN27_001063"
     CDS             complement(45250..45438)
                     /locus_tag="OHN27_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCQ2285548.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(45538..45948)
                     /gene="menI"
                     /locus_tag="OHN27_001064"
     CDS             complement(45538..45948)
                     /gene="menI"
                     /locus_tag="OHN27_001064"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCQ2285549.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(45945..49001)
                     /locus_tag="OHN27_001065"
     CDS             complement(45945..49001)
                     /locus_tag="OHN27_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCQ2285550.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELSETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            49215..50327
                     /gene="ydiK"
                     /locus_tag="OHN27_001066"
     CDS             49215..50327
                     /gene="ydiK"
                     /locus_tag="OHN27_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCQ2285551.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            50513..50620
                     /gene="rprA"
                     /locus_tag="OHN27_001067"
     ncRNA           50513..50620
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OHN27_001067"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            50756..51112
                     /locus_tag="OHN27_001068"
     CDS             50756..51112
                     /locus_tag="OHN27_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCQ2285552.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            51212..52426
                     /locus_tag="OHN27_001069"
     CDS             51212..52426
                     /locus_tag="OHN27_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2285553.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCKFPPHPGRRSPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            52653..53918
                     /locus_tag="OHN27_001070"
     CDS             52653..53918
                     /locus_tag="OHN27_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2285554.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGIQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            53930..54796
                     /gene="ydiB"
                     /locus_tag="OHN27_001071"
     CDS             53930..54796
                     /gene="ydiB"
                     /locus_tag="OHN27_001071"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCQ2285555.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVY
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            54827..55585
                     /gene="aroD"
                     /locus_tag="OHN27_001072"
     CDS             54827..55585
                     /gene="aroD"
                     /locus_tag="OHN27_001072"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCQ2285556.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYSEADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVTDLRTVLTILHQA"
BASE COUNT        13418 a        14408 c        13529 g        14244 t
ORIGIN      
        1 ctttcattat gatttgctca tatttggtag aacatgtaac catggattca catatgccat
       61 atactttgac catgagggat gcttgcgtgg cgtttcatgg tgaacaggag atttttcaat
      121 gattaaacgc gtattggttg tttcaatggt aggtctgtct cttgtcggtt gtgttaataa
      181 cgacaccctg tcaggggatg tttataccgc ttctgaagcg aaacaagtac agaatgtcag
      241 ctatggcacc atcgttaacg tacgtccggt acagattcag ggcggtgatg attccaacgt
      301 tatcggtgca attggcggtg ctgttcttgg tggtttcctg gggaatactg ttggtggcgg
      361 aaccgggcgt tctctggcta ctgcagcagg cgctgttgca ggtggcgtag ctggtcaggg
      421 cgtacagagt gcaatgaaca aaacgcaggg tgtcgagctg gaaattcgta aagacgatgg
      481 taataccatc atggtggtac agaaacaagg caacactcgt ttctctccgg gccaacgtgt
      541 cgtactggcc agcaatggca gtcaggtgac cgtttctccg cgctaaataa gtttgcgtgt
      601 ggtcaggtta ctgaccacac gcccccttca tttcaccctt tggcctgtaa ctcaatgata
      661 ttatgctcaa gttttgcgat gagcgtaatc agttgctcca gttcctctgc ggagatgcca
      721 tgtaatattt ccgcgcgggt tttgttaata acagcttcca tttcgctgat cagcggctct
      781 gccttttccg tcagtttaat acgtttagcc cgacgatcgc tggcacaagt ttgacgcgaa
      841 attaaccctt tttcttccag ttggtccaga gtacggacca gtgatggctg ctcgatgcca
      901 atcgcttttg ccagttgaat ttgcgactgg tctggaggta actgatggat attgtgtaac
      961 gtaacccaat gggtttgtgt taactccagc ggtttcaggc gatggtctat cagagcacgc
     1021 catatgcgca ccaaccgtgc cagatcagaa cctagtggcg attccaattt catctcctta
     1081 taattagctt gctaagatat tatgcggctt ttagaatagt gtgcagcaat tgtattgcta
     1141 aaacaaatgt attgctgcat ttggttaccg tcagacatat ttttcagaaa ttgcgcgtaa
     1201 atttttcgca cttaaagaat atttattaat ctaacgcaat atattcggtc gtaaaggaat
     1261 ctactttgtg aagtttattc tcaatgcaac aggattgccc ttacaagatc tggtgttcgg
     1321 tgcgtccgtc tactttcctc cgtttttcaa agccttcgcg tttggattcg tcatctggct
     1381 tgtcgtacac cgcctgcttc gtggctggat ctacgccggt gacatctggc atcccttgtt
     1441 aatggattta tcgctgtttg cgatttgcgt ttgccttgct ctggcaatac tgattgcgtg
     1501 gtaactatgt caattaaaac aattaagtat ttctcaacaa tcattgtagc ggtagttgcg
     1561 gttcttgccg gatggtggct gtggaattat tacatgcaat ctccgtggac acgcgacggg
     1621 aaaatacgcg cagaacaggt ttctatcacc ccacaggtgt cgggacgtat tgttgagctg
     1681 aatataaaag acaaccagct ggttaacgcg ggggatcttc tgctcacgat cgataaaacg
     1741 ccctttcaga tcgccgagct gaacgcacag gctcagctgg cgaaagcgca atctgacctc
     1801 gcaaaagcca ataacgaagc gaatcgtcgt cgtcatctct cacaaaattt tatttctgcc
     1861 gaagaactcg ataccgctaa cctcaatgtt aaagcgatgc aggccagtgt tgatgccgca
     1921 caggcgacgc tgaaacaggc acaatggcaa ctggcgcaaa cggaaattcg cgccccggtg
     1981 agtggatggg tgactaacct tactacccgc atcggcgatt acgctgatac cgggaaaccg
     2041 ctgtttgccc ttgtcgacag ccactcgttt tacgtcattg gttattttga agaaaccaag
     2101 ttgcgccata tccgcgaagg tgcaccagca caaattaccc tctatagtga taacaaaacg
     2161 ttacagggtc acgtttccag tatcggtcgg gcgatttatg atcagagcgt tgaaagtgac
     2221 tcaagcctta ttccggatgt taaacctaac gtcccctggg ttcgactcgc ccaacgcgtt
     2281 cccgtccgct ttgcgctgga taaagtccca ggcgatgtca cgttggtgtc gggcaccacc
     2341 tgtagcattg ccgtaggtca ataatgaacg catcgtcatg gtccttgcgc aatttgccct
     2401 ggttcagggc cacgctggcg caatggcgtt atgcgttacg caataccatt gccatgtgtc
     2461 tggcgctgac ggttgcctat tatttaaatc tggatgaacc ctattgggcg atgacctcgg
     2521 ctgcagtggt tagctttccc accgttggcg gtgttatcag caaaagcctc ggacgcatcg
     2581 ctggcagttt gctcggagcc attgcggcac tgcttcttgc cgggcatacg ctcaatgagc
     2641 cgtggttttt tctattgagc atgtcggcgt ggcttggctt ttgtacctgg gcctgtgcgc
     2701 acttcacgaa taacgtcgcg tatgcatttc aactggcggg ctacacggct gccatcatcg
     2761 cctttccgat ggttaatatt actgaggcca gccagctgtg ggatatcgct caggcgcgcg
     2821 tttgcgaggt gattgtcggc attttgtgcg gcggcatgat gatgatgatc ctgcctagca
     2881 gttccgatgc tacagccctt ttaaccgcat tgaaaaacat gcacgcccga ctacttgaac
     2941 atgccagttt actctggcag cctgaaacaa ccgatgccat tcgtgcagca catgaagggg
     3001 tgattgggca gatactgacc atgaatttgc tgcgtatcca ggctttctgg agccactatc
     3061 gttttcgcca gcaaaacgcg cgccttaatg cgctgctcca ccagcaatta cgtatgacca
     3121 gtgtcatctc cagcctgcga cgtatgttgc tcaactggcc ctcaccgcca ggtgccacac
     3181 gagaaattct cgaacagttg ctgacggcgc tcgccagttc gcaaacagat gtttacaccg
     3241 tcgcacgtat tatcgccccg ctacgcccga ccaacgtcgc cgactatcgg cacgtcgcct
     3301 tctggcagcg actacgttat ttttgccgcc tttatctgca aagtagtcag gaattacatc
     3361 gtctgcaaag cggtgtagat gatcatacca gactcccacg gacatccggc ctggctcgtc
     3421 ataccgataa cgccgaagct atgtggagcg ggctgcgtac attttgtacg ttgatgatga
     3481 ttggcgcatg gagtattgct tcgcaatggg atgccggtgc caatgcatta acgctggcag
     3541 caattagctg cgtactctac tccgccgtcg cagcaccgtt taagtcgttg tcacttctga
     3601 tgcgcacgct ggtgttactt tcgctattca gctttgtggt caaatttggt ctgatggtcc
     3661 agattagcga tctgtggcaa tttttactgt ttctctttcc actgctggcg acaatgcagc
     3721 ttcttaaatt gcagatgcca aaatttgccg cattgtgggg gcaactgatt gtttttatgg
     3781 gttcttttat cgctgtcact aatcccccgg tgtatgattt tgctgatttt cttaacgata
     3841 atctggcaaa aatcgttggc gtcgcgttgg cgtggttagc gttcgccatt ctgcgtccag
     3901 gatcggatgc tcgtaaaagc cgccgccata ttcgcgcgct gcgccgggat tttgtcgatc
     3961 agctaagccg ccatccaaca ctgagtgaaa gcgaatttga atcgctcact tatcatcacg
     4021 tcagtcagtt gagtaacagc caggatgcgc tggctcgccg ttggttatta cgctggggtg
     4081 tagtgctgct gaactgttct catgttgtct ggcaattgcg cgactgggaa tcgcgttccg
     4141 atccgttatc gcgagtacgg gataactgta tttcactgtt gcggggagtg atgagtgagc
     4201 gtggcgttca gcaaaaatca ctggcggcca cacttgaaga attacagcgg atttgcgaca
     4261 gccttgcccg tcatcatcaa cctgccgccc gtgagctggc ggcaattgtc tggcggctgt
     4321 actgctcgct ttcgcaactt gagcaagcac caccgcaagg tacgctggcc tcttaattac
     4381 ttaattacac cacaggcata gcgttcaccg ccaccgccca gcggtttagg ttgatcggac
     4441 atattatcgc cgccaacgtg gaccatcagc gctttgtctt tgatttcatc cagtgatttc
     4501 agacgaggcg cgatgacggc atcggtagct ttgccgtcat tattgacgac cagtgcaggc
     4561 agatcgccta aatgcccggc accttctggc ccttcatgtt taccggtatt ttgtggatca
     4621 agatgcccgc ctgcggattc cgcggcgctg gctttgccat ctttggtggc tggctggcag
     4681 cttcctttgg catgaatatg gaagccatgt tcaccggggg gtaatgcttt cagatcgggc
     4741 gaaaactcca gacctttatc ggtttcagta atggtgacgc taccaattga ctgccctacc
     4801 ccttgcgacg tgacgaggtt catctcgact ttttcactgg cagcttgtgc gccggttgca
     4861 acaaccagcg ccagaatagc cagactaaaa cgtttcatag gacctccgtt cattgtgcat
     4921 tcctgaataa gtgtaaacca gtgacacatt tttgaacggc ggctattcct aaaagtgctt
     4981 acggtacgtc gtaccccagt gccgctttac ggatacgaaa ccattgttga cgggtcattt
     5041 tcagtgtttc tgcttcgaca gctgcccgta cgcgctcaat tttacctgaa ccgataattg
     5101 gcagcggctg cgatggtaaa cgtaataccc aggcgtaaac cacctgttca atcgagcccg
     5161 cgtttaactc ctctgccacc acagccagtt catcacgcag cggctggaaa taatcatcat
     5221 taaacagacg accaccacca aggcaggacc acgccatcgg acgaacacgc agttgttgta
     5281 gttggtcgag cgtgccatcc agcagtaacg gctgatgcac cggggatatt tccacctgat
     5341 tagtggcaag ggtaaacggc agacgtgatt gcaacagggc aaattgcgca ggcgtaaagt
     5401 tcgatacgcc aaaatgtcgc actttgccgc tctgatgcag atgtttgaac gcgtccgcca
     5461 cttcatcggc atccattaac gggtctggtc ggtggattaa cagcaaatcc agatgatcgg
     5521 tcgcgagatt aattagcgac tgttcggcgc tcttaatgat gtgatcgcgg tcagtgatgt
     5581 aatgaccaat gacgttttct tcacgcgcgg tcgtcgcgat accgcattta ctgacgattt
     5641 ccatccgttc acgcaggtga ggtgccagtt tcagtgcctc gccaaacgcc gcttcgcact
     5701 gatagccacc ataaatatca gcatggtcca cggtggtcac gccgagatcc agatgctctt
     5761 caataaaact gaccagctgg cgggcggaca tattccagtc catcaatcgc cagtagccca
     5821 tcacaaaacg ggaaaactcc gggccttgcg gcgcaatagt aatacgctga accataatcg
     5881 cttcctctta tcagatatga gaggagtata cgcaagatta ggttcaaaag agtgatggtt
     5941 gctccggttc gtctgatgac gctggcttat ttgcgcgtaa tttgcgcatt aatcgctgcc
     6001 gacaaaggcg tagcacctct tgtttttcgc catcgctcat tttattccag ttaaaacgct
     6061 catcacgact acgaaaacag ccgcgacaaa acccgcgttc gtcagactgg caaattcccc
     6121 ggcacgggct ctggacggga aagaactcta attgctccgc cacttcgccc tcctcagata
     6181 agattattac cattattgaa gctgttaatg tccaaagtag caactttgct tgcactagac
     6241 cgactggtct actacactcc aacgcatgaa caaacacacc gaacatgata ctcgcgaaca
     6301 tctcctggcg acgggcgagc aactttgcct gcaacgtgga ttcaccggga tggggctaag
     6361 cgaattacta aaaaccgctg aagtgccgaa agggtccttc tatcactact ttcgctctaa
     6421 agaagcgttt ggcgttgcca tgcttgagcg tcattacgcc gcatatcacc agcgactgac
     6481 tgagttgctg caatccggcg aaggtaacta ccgcgaccgc atactggctt attaccagca
     6541 aacactgaac cagttttgcc aacatggaac catcagtggt tgcctgacag taaaactctc
     6601 tgccgaagtg tgcgatctgt cagaagatat gcgcagcgcg atggataaag gtgctcgcgg
     6661 cgtgatcgcc ctgctctctc aggcgctgga aaatggccgt gagaaccatt gtttaacctt
     6721 ttgtggcgaa ccgctgcaac aggcacaagt gctttacgca ctgtggcttg gcgcgaatct
     6781 gcaggccaaa atttcgcgca gtttcgagcc actggaaaac gcgctggccc atgtaaaaaa
     6841 cattattgcg acgcctgccg tttagcaggc attttttatc accagacgac cgggagcctt
     6901 tatgtcatct gaaaaactgt attccccact gaaagtgggc gcgatcacgg cggcaaaccg
     6961 tatttttatg gcaccgctga cgcgtctgcg cagtattgaa ccgggtgaca ttcctacccc
     7021 gttgatggcg gaatactatc gccaacgtgc cagtgccggt ttgattatta gtgaagccac
     7081 gcaaatttct gcccaggcaa aaggatatgc aggtgcgcct ggcatccata gtccggagca
     7141 aattgccgca tggaaaaaaa tcaccgctgg cgttcatgct gaaaatggtc atatggccgt
     7201 gcagctgtgg cacaccggac gcatttctca cgccagcctg caacctggcg gtcaggcacc
     7261 ggtagcgcct tcagcactta gcgcgggaac acgtacttct ctgcgcgatg aaaatggtca
     7321 ggcgatccgt gttgaaacat ccatgccgcg tgcgcttgaa ctggaagaga ttccaggtat
     7381 cgtcaatgat ttccgtcagg ccattgctaa cgcgcgtgaa gccggttttg atctggtaga
     7441 gctccactct gctcacggtt atttgctgca tcagttcctt tctccttctt caaaccatcg
     7501 taccgatcag tacggcggca gcgtggaaaa tcgcgcacgt ttggtactgg aagtggtcga
     7561 tgccgggatt gaagaatggg gtgccgatcg cattggcatt cgcgtttcgc caatcggtac
     7621 tttccagaac acagataacg gcccgaatga agaagccgat gcactgtatc tgattgaaca
     7681 actgggtaaa cgcggcattg cttatctgca tatgtcagaa ccagattggg cggggggtga
     7741 accgtatact gatgcgttcc gcgaaaaagt acgcgcccgt ttccacggtc cgattatcgg
     7801 cgcaggtgca tacacagtag aaaaagctga aacgctgatc ggcaaagggt taattgatgc
     7861 ggtggcattt ggtcgtgact ggattgcgaa cccggatctg gtcgcccgct tgcagcgcaa
     7921 agctgagctt aacccacagc gtgccgaaag tttctacggt ggcggcgcgg aaggctatac
     7981 cgattacccg acgttgtaat ccaacattgc gagcggcgta aagccgccgc tatactaaaa
     8041 caacattttg aatctgttag ccattttgag gataaaaaga tgcgtcttct tcataccatg
     8101 ctgcgcgttg gcgatttgca acgctccatc gatttttata ccaaagtgct gggcatgaaa
     8161 ctgctgcgta ccagcgaaaa cccggaatac aaatactcac tggcgtttgt tggctacggc
     8221 ccggaaaccg aagaagcggt gattgaactg acctacaact ggggcgtgga taaatacgaa
     8281 ctcggcactg cttatggtca catcgcgctt agcgtagata acgccgctga agcgtgcgaa
     8341 aaaatccgtc aaaacggggg taacgtgacc cgtgaagcgg gtccggtaaa aggcggtact
     8401 acggttatcg cgtttgtgga agatccggac ggttacaaaa ttgagttaat cgaagagaaa
     8461 gacgccggtc gcggtctggg caactaatct cctgccgggc gtgaactcat cgcgcccgca
     8521 tctttactgc atcgacaagt aatatttgtc ataatgcgcg ctgcaattta tccgtattaa
     8581 gagaatcaga tgtccgataa cgctcaactt accggtctgt gcgaccgttt tcgtggtttt
     8641 tatcctgttg tgatcgatgt tgaaacagcc ggatttaacg ccaaaaccga tgcgctgctt
     8701 gagattgccg ccatcaccct gaaaatggat gaacaaggct ggctgatgcc ggacaccaca
     8761 ttacatttcc acgtcgaacc atttgtcggc gcaaatttgc aaccagaagc cctcgccttc
     8821 aacggcattg acccgaacga tcccgatcgc ggcgcggtca gcgaatacga ggcgctgcac
     8881 gaaattttta aagttgtacg taaaggtatt aaagcgagcg gctgtaaccg cgccattatg
     8941 gtggcgcaca atgccaattt tgatcacagc tttatgatgg ccgccgcaga acgcgcctca
     9001 ctgaaacgta acccgttcca ccctttcgcc acttttgaca ctgctgcact ggccgggctg
     9061 gcactcggac aaaccgtatt gtcaaaggct tgccagaccg ctggcatgga cttcgacagc
     9121 acccaggcgc actccgcgct gtacgacacc gaacgcactg ctgtgctgtt ttgtgaaatc
     9181 gtcaaccgct ggaaacgtct gggaggctgg ccgctacctg ccgccgaaga ggtgtaatcg
     9241 agtcgatgcc tgatgacatg caatgattca ggcatctata gtgaggctat tccacgcatc
     9301 ctgcatgata ttcacgggga atagcgttaa tggcagataa tccagaccct tcatcgctcc
     9361 tgccggacgt gttttcaccg gcgacccgcg actggtttct tcgcgccttt aaacagccga
     9421 ccgctgtcca gccgcaaacc tggcatgtgg cggcgcgaag cgaacatgcg ctggtgattg
     9481 caccgaccgg ctccgggaaa acgctggcag catttctcta cgccctcgat cggctcttcc
     9541 gcgaaggcgg cgaagatacc cgcgaggcgc ataagcgtaa aacctcacgc atcctctata
     9601 tttcaccgat aaaagccctg ggcaccgacg ttcagcgcaa cttgcagatc ccgttgaagg
     9661 gtattgccga tgaacggcgg cggcgcggcg aaacggaagt caatcttcgc gtagggatcc
     9721 gtactggcga tacgcctgca caggaacgca gcaaactcac ccgtaatccg ccggatattc
     9781 tgatcaccac acccgaatca ctctatctga tgctgacctc ccgcgcgcgc gaaacgctac
     9841 gcggcgtcga aacggtaatt attgatgaag tccacgcggt agcgggcagt aaacgtggtg
     9901 cgcatctggc gttaagtctg gagcggctcg atgcgctgct ccacacctca gcacagcgaa
     9961 ttggcctttc tgccactgtg cgctcagcca gcgatgtggc agcatttctt ggtggcgatc
    10021 gcccggttac ggtagtcaac ccgcccgcaa tgcgccatcc gcagatacga attgtcgtac
    10081 cggtcgccaa tatggatgat gtctcatcgg tcgccagcgg caccggcgaa gacagccatg
    10141 ccggccggga aggctccatc tggccatata ttgaaacggg tatccttgat gaagtgttgc
    10201 gccatcgctc gaccattgtc tttactaatt cacgcgggct ggcggaaaaa ctgacggcac
    10261 gattaaatga gctttacgcc gcacgcttac agcgttcccc gtctatcgcc gttgatgcgg
    10321 cccatttcga gtcgacctcc ggcacaacct ctaaccgtgt acaaagtagc gacgttttta
    10381 ttgcccgctc acaccacggc tccgtctcta aagaacaacg agcaatcacc gaacaggcgc
    10441 tgaaatcggg tgaattacgc tgcgtggtcg caacctccag tcttgaactg gggattgata
    10501 tgggcgcggt ggatctggtg attcaggtgg caacgccgct ttctgttgcc agtgggttac
    10561 aacgcattgg tcgcgccgga catcaggttg gcggtgtatc taaagggctg tttttccccc
    10621 gtacccggcg tgatttagtc gattccgcag tcattgtaga gtgtatgttc gcaggcaggc
    10681 tggaaaacct gacaccaccg cataatcctc tcgacgtcct tgcgcagcaa accgttgccg
    10741 ccgcggcgat ggatgcatta caggtagacg aatggtactc ccgcgtacgc cgtgccgcac
    10801 cgtggaaaga tctgccaaga cgtgtttttg acgccacgct ggatatgctt tccgggcgct
    10861 atccctctgg cgatttttct gcttttcgcc ccaaactggt ctggaacagg gagaccggga
    10921 tattgaccgc ccgacctggc gctcaattgt tggcggttac cagcggcggc accattccgg
    10981 atcgtggcat gtatagcgtg ttattacccg aaggtgaaga aaaggccggt tcgcggcggg
    11041 tgggtgaact ggatgaggag atggtatatg agtcgcgggt gaacgacatt atcactctcg
    11101 gcgctacctc atggcggatc cagcaaatca cccgcgatca ggtgattgtg actcctgctc
    11161 cgggtcgttc tgcccggctc cccttctggc gtggtgaagg taacggacgt ccggctgaat
    11221 taggcgagat gatcggcgat tttcttcatt tgctggcgga tggcgcgttc ttttccggga
    11281 ctattccccc gtggctggca gaagaaaata cgatcgccaa tattcagggg ttgattgagg
    11341 agcagcgcaa cgcgacgggc atcgttccgg ggagtcgcca tctggtcctc gaacggtgcc
    11401 gtgatgaaat tggtgactgg cgtattattt tgcactctcc ctatggaaga cgggtgcatg
    11461 aaccctgggc gctggcgatt gccgggcgaa tacatgcgct atggggcgct gacgcgtcgg
    11521 tggtcgccag tgatgacggc attgttgcac gtattcctga caccgatggt aaattgcccg
    11581 atgccgcgat ttttttgttt gaaccagaaa agttgctgca aattgtccgc gaggcggtag
    11641 gcagctcggc acttttcgcc gcccgttttc gcgaatgcgc cgcgcgggca ttattaatgc
    11701 cggggcgcac tccgggccat cgcaccccgc tttggcaaca acggctgcgc gccagtcagt
    11761 tgctggaaat cgctcaggga tatccggatt ttccggtcat tctcgaaacc ctacgcgaat
    11821 gtctgcaaga tgtttatgat cttcccgcac tggaacgttt gatgcgtcgc ctgaacggtg
    11881 gcgaaattca aatatccgat gtaacgacca ctacgccctc gcctttcgcc acaagtttat
    11941 tgttcggcta tgtcgcggaa tttatgtacc agagcgacgc cccgctggca gagcgccggg
    12001 catccgtact gtcgctggac agcgtgttac tgcgcaatct actcggacag gtcgatccgg
    12061 gggaattact cgacccgcag gtcattcgcc aggtggaaga agagttgcaa cgactggctc
    12121 ctggcagaag agcgaaaggt gaagaaggat tgttcgacct gctgcgcgaa ctggggccaa
    12181 tgaccgttga agacctggcg caacggcata caggcagcag tgaagaggtt gcgtcgtatc
    12241 tggaaaatct tcttgcagta aaacgaatct tcccagcgat gattagcgga caggagcgtc
    12301 ttgcctgtat ggatgatgcc gccaggctgc gtgatgccct cggcgtacga ctaccagagt
    12361 cattgccaga gatttattta catagagtca gttacccgct tcgcgacctc tttctgcgct
    12421 atctccgggc tcatgctctg gtcacggctg aacaactggc tcatgagttt agtctcggta
    12481 ttgccattgt cgaagagcag cttcagcaac tgcgtgaaca gggtctggtg atgaatctgc
    12541 aacaagacat ctgggtgagc gatgaagtat ttcgtcgtct gcgtttgcgc tcgctgcaag
    12601 ccgccagaga agcgacgcgt cccgttgcag ccacgaccta tgcgcgattg ctgctggaac
    12661 gtcagggcgt attacccgcc accgatggta gcccggcgct ctttgcctca acatcgccag
    12721 gcgtttatga gggcgtagat ggcgtgatgc gggtgatcga acagcttgcc ggagtcggtt
    12781 tacccgcctc actctgggaa agccagatcc tgcctgcccg cgtacgcgac tattcgtcag
    12841 aaatgctcga tgaattactg gcaaccggtg cggttatctg gtcggggcaa aaaaaactgg
    12901 gtgaagatga cggcctggtg gcactgcatc tacaggaata tgctgcagaa tcgttcactc
    12961 ccgccgaagc ggatcaggcg aatcgttcgg cgctgcaaca agcgatagtc gctgttctgg
    13021 ctgacggagg agcctggttt gcacaacaaa tcagccaacg gatacgcgac aaaatcggcg
    13081 aatcggttga tctctctgcc ctgcaagagg cgttatgggc gctggtctgg caaggcgtca
    13141 tcaccagcga catttgggca ccgttacgcg ccctcacccg cagcagttcc aacgcacgca
    13201 cctcaactcg ccgcagtcac cgggctcgtc gtggacgtcc tgtctatgcg caacccgtct
    13261 cgccgcgggt atcttacaac acaccaaatc tggctggacg ctggtcgtta ttgcaggtgg
    13321 agccactaaa cgataccgaa aggatgctgg cgctggcgga aaatatgctc gaccgctacg
    13381 gcatcatcag tcgtcaggcg gtgatagccg aaaatatccc tggcgggttt ccatcgatgc
    13441 aaacgctttg tcgaagtatg gaagactccg ggcgaattat gcgaggtcgt tttgtagaag
    13501 gtctgggtgg cgcgcaattc gctgaacgtc tgactattga ccgattgcgc gatctggcga
    13561 cacaagccac gcaaacgcgc cactatacac cagtggcgct ctctgccaac gatccggcta
    13621 atgtgtgggg aaatcttctg ccctggcctg cacatccggc aacgctggtt ccaacgcgtc
    13681 gggcgggtgc gctggtggtc gtttctggcg gcaaattgtt actctatctg gcgcaaggtg
    13741 gcaaaaaaat gctggtctgg caggaaaaag aggaattact cgccccagag gttttccacg
    13801 cgctgactac cgcactgcgt cgcgaaccac ggctgcgctt tacgctaaca gaagtgaatg
    13861 atctaccggt ccggcaaacg ccgatgttta cgctgctgcg tgaggcggga ttttcaagtt
    13921 cgccacaagg gctggattgg ggatagagaa aggactgacg gatgcccgtt cgcatccgtc
    13981 agtattgcag gacggattat tccgcgtccg gctcttcaga cttgtattta gcggcagttt
    14041 ctttgatcag ctgctgcagt tcgccacgct gatacatttc gatcacgata tcacaaccgc
    14101 cgaccagctc gccgtcaacc cacagttgcg ggaaggtcgg ccagttagca tatttcggca
    14161 gttccgcacg aatgtccgga ttctgcagaa tatcaacata ggcaaaacgt tcgccacatg
    14221 cggcaagcgc ctggactgcc tgggcagaga aaccgcagct cggcagtttc ggtgaacctt
    14281 tcatgtacag caggatcggg ttttcagcaa tctggcgttg gattttttcg atagtggtgc
    14341 tcattgtctt gcttcctcaa acttctttac ggcagtaatc tgacattgta gcgggtcagt
    14401 gcggcatcgg aaaataacat tttcatcacg cttttgctat tttatccctt tgctctattt
    14461 gttgcatttc aaatattcgt ttcttctctg atgccggcaa actggcgtat ttataaccat
    14521 ttttagcttt cactgctgca tttttttgcg ctcgccaacg aaacgtattt tttaacaata
    14581 aaagctatta actttctctt cttctatgca ttagaatcat caagttttgt aaatcagacg
    14641 caggcatgat agacctgcct taacagaggg acgctcagtg gcgcggataa accgtatttc
    14701 gatcacgctc tgtgctttac tttttaccac cctgccttta acgcctatgg cccatgcttc
    14761 aaagcaagcc agggagagtt ctgctaccac tcatatcacc aaaaaagcag ataaaaagaa
    14821 aagcacggca accaccaaaa agacccagaa aacagcgaaa aaagccgcca gtaaaagtac
    14881 gaccaaaagc aaaaccgctt cttccgttaa aaaatcttcc attaccgctt ctaaaaacgc
    14941 caaaactcgc agcaaacacg ccgtcaataa aacggcctca gccagcttta ccgaaaagtg
    15001 taccaaacgt aagggctata aatcgcattg tgtgaaagtc aaaaatgccg cgtcaggaac
    15061 tcttgccgac gcgcacaaag cgaaggtgca aaaagctacg aaagtggcaa tgaataaact
    15121 gatgcagcaa attggtaagc catatcgttg gggtggcagc tcaccgcgta ccggttttga
    15181 ttgcagcggc ctggtttatt acgcttataa agatttggtg aaaattcgta ttccgcgtac
    15241 ggcgaatgaa atgtatcacc tgcgtgatgc agcgccaatc gaacgtagtg aactgaaaaa
    15301 cggcgacctg gtctttttcc gtactcaggg acgcggcaca gccgatcatg tcggcgtgta
    15361 tgtcggcaac ggcaaattta ttcagtcacc gcgcacaggt caggaaattc aaatcacttc
    15421 tctcagtgaa gactactggc agcgccacta tgttggcgct cgtcgggtaa tgaccccaaa
    15481 aacacttcgc taaaacttta ccctgttgtt acggcaacag ggtaagttca tcttttgtct
    15541 caccttttaa tttgctaccc tatccatacg cacaataagg ctattgtacg tatgcaaatt
    15601 aataataaag gagagtagca atgtcattcg aattacctgc actaccatat gctaaagatg
    15661 ctctggcacc gcacatttct gcggaaacca tcgagtatca ctacggcaag caccatcaga
    15721 cttatgtcac taacctgaac aacctgatta aaggtaccgc gtttgaaggt aaatcactgg
    15781 aagagattat tcgcagctct gaaggtggcg tattcaacaa cgcagctcag gtctggaacc
    15841 atactttcta ctggaactgc ctggcaccga acgccggtgg cgaaccgact ggaaaagtcg
    15901 ctgaagctat cgccgcatct tttggcagct ttgccgattt caaagcgcag tttactgatg
    15961 cagcgatcaa aaactttggt tctggctgga cctggctggt gaaaaacagc gatggcaaac
    16021 tggctatcgt ttcaacctct aacgcgggta ctccgctgac caccgatgcg actccgctgc
    16081 tgaccgttga tgtctgggaa cacgcttatt acatcgacta tcgcaatgca cgtcctggct
    16141 atctggagca cttctgggcg ctggtgaact gggaattcgt agcgaaaaat ctcgctgcat
    16201 aataactgat ggcaaatgca gcattgcctg aagcgctacg cttatcaggc ctacgcggat
    16261 catcgatgta ggtcggataa ggcactcgcc gcatccgaca agcgctgcat gatacgacgc
    16321 ctaacgcgtc ttttcatgtc aacgcggatg agtcgttaac accgcatccg gcaagataaa
    16381 tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag
    16441 ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat
    16501 gccagtcccg cgacaatcgc tcccatcacc ggcacaaagc tgtatcccag cccagcggaa
    16561 attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc accaatattg
    16621 actgatgaag acagacccgg cgcttcactg gcgacacgca tcacgcgcat ctgtaacggc
    16681 ggtacgaccg caaaggttgc tgcgccccac accaccatgc taatagctgc gccgaactca
    16741 ttgcgggcca ggaacgggat tgccagcata atcaccatca gcagcaacaa aaagcctttc
    16801 aacgtgccgt taactgaacg atctgccagt ttgccgccga gatagttacc gatagagaat
    16861 ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt
    16921 tgcagtaccg gagagatata ggtgtagaga gtaaacattg caccagctcc cagtaccgtc
    16981 gtcagcaatg cagacagcac ctgcggacgc attaataccg ccagctcttt tttcacttca
    17041 ggtcgtgccc ctgcaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact
    17101 cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc
    17161 gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg
    17221 ctggcctgtt tatgttttgg caccacgctt gcggccacga ctgaacccaa accaaaaaat
    17281 gctccgtgat tcaggctggt caaaatgcgt gaaagcatca gggtcatata atccggcgcg
    17341 atggcggaaa gaacgttgcc tagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg
    17401 cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc ccaccattac gccaactgca
    17461 taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg
    17521 ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccagc
    17581 gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc attacgcgat
    17641 attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata acaaaaagag
    17701 aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga acgcgctccc
    17761 tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ctacgatcaa accgaggacg
    17821 ataatcgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc tcctttttta
    17881 ttaccacaca aaaagtgata ttacgcattt ttacacactg tgatgaaaaa atctcccgtc
    17941 atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc caaaattcca
    18001 cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg tcgtttttcc
    18061 ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt taccttgcga
    18121 ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat aaaagatgta
    18181 gcgaaacgag caaacgtttc cactacaact gtgtcacacg tgatcaacaa aacacgtttc
    18241 gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca ctactcccct
    18301 agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt gctggcgacc
    18361 agcagcgaag cggcctattt tgccgagatc attgaagcgg ttgaaaaaaa ttgcttccag
    18421 aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca gcgggcttat
    18481 ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc tgagtaccca
    18541 gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt gatggactgg
    18601 ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgtttga aggcggctac
    18661 atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgttat ccccggcccg
    18721 ctggaacgta acactggcgc aggccgcctt gccggtttta tgaaggcgat ggaagaagca
    18781 atgatcaagg tgccggaaag ctggattgtg cagggtgatt ttgaacctga atccggttat
    18841 cgcgccatgc agcaaatcct gtcgcagccg catcgcccta ctgccgtctt ctgtggtggc
    18901 gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggcctgcg cgtcccgcag
    18961 gatgtttcgc tgatcggtta tgataacgtg cgcaacgccc gctattttac gccggcgctg
    19021 accacgatcc atcagccaaa agactcgctg ggtgaaacag cgttcaacat gctgttggat
    19081 cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg cttgattgaa
    19141 cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc cgttgcggga
    19201 gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgcc gagatactca
    19261 agcaaccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca acatgccgaa
    19321 tccggaaagg ggttttccag ttctaatgct acccacttcc cctcattaag ccacggtttg
    19381 gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt tgccgatgat
    19441 tcccagtcag gcacgacaag tcttttttgg ttatccagca accaggtaat acgcttaggt
    19501 agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt atcatcgctg
    19561 aatgggccat ccatcaacgc cagcgggtgg tggctggcaa caacgcaact ccagcttagc
    19621 atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt tgcgccaatc
    19681 gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac ttcctgaaag
    19741 acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg ccgtgtacgt
    19801 tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc gttcgctatc
    19861 tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc ttctttgaga
    19921 aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa gagcggcacc
    19981 gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac gcgatgcagc
    20041 tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac ttcgagtgaa
    20101 tattctgacc acatagtctg cctacaaaat ttttgaaacc agtcatcaaa tattaccgtt
    20161 tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga gatttagaga
    20221 atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag cgtactcggt
    20281 tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga cctgcaaacg
    20341 cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc cgcagcccag
    20401 cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt aatcggcctg
    20461 acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc tacgctgctg
    20521 gtattgcgtt ttgtacaggc tgtgggggtc tgcgccgcgg cggttatctg gcaagcgtta
    20581 gtgacggatt attatccttc acagaaagtc aaccgtattt ttgcgaccat catgccgctg
    20641 gtgggtctgt ctccggcact tgctcccctg ttaggaagct ggctgctggt ccatttttcc
    20701 tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct gcctattttc
    20761 tggctcaaac ccacgacgaa ggcccgtaac aatagtcagg atggtctgac ctttaccgac
    20821 ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat atgcggcctg ttcagccagt
    20881 ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta cagcccggca
    20941 gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg ttatggctgt
    21001 cgcgccgcac tgcagaaatg gcaaggcaag cagttattac cgtggttgct ggtgctgttt
    21061 gctgtcagcg tcattgcgac ctgggctgca ggcttcatta gccatgtgtc gctggtcgaa
    21121 atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc tattgttgtc
    21181 gcccaggcgc tgcgtccctt cccacacgca actggtcgcg ccgcagcttt gcagaacact
    21241 ctacaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggctgat cagtatcagc
    21301 acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt cgcgctgggt
    21361 tacatgatgc aacgttgtga agaagttggc tgccagaatc atggcaatgc cgaagtcgct
    21421 catagcgaat cacactgatc tatatcgata tacttatact taggctgcta acaaaatttt
    21481 gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct acgataaata
    21541 ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat ttcggcaagg
    21601 gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt ctgcgagatt
    21661 tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg accagtgatg
    21721 gagaaactat gagttcatcg tgtatagaag aagtcagcgt accggatgac aactggtacc
    21781 gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct gccccggcgg
    21841 atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaagaaggc tctttggggt
    21901 taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg ttttttagca
    21961 aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac acgctgcgta
    22021 ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata gtcggcaaag
    22081 agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc atgcaatatt
    22141 cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg aagctcaaaa
    22201 tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt ggctgcggct
    22261 ggggcggact ggcacactac atggcatcta attatgacgt aagcgtggtg ggcgtcacca
    22321 tttctgccga acagcaaaaa atggctcagg aacgctgtga aggcctggat gtcaccattt
    22381 tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct gtggggatgt
    22441 tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat cgtaatttga
    22501 aaccggaagg catattcctg ctccatacta tcggttcgaa aaaaaccgat ctgaatgttg
    22561 atccctggat taacaaatat atttttccga acggttgcct gccctctgta cgccagattg
    22621 ctcaatccag cgaaccccac tttgtgatgg aagactggca taacttcggt gctgattacg
    22681 atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa attgcggata
    22741 actacagtga acgctttaaa cgaatgttta cctattatct gaatgcctgt gcaggtgctt
    22801 tccgcgcccg tgatattcag ctctggcagg tcgtgttctc acgcggtgtt gaaaacggcc
    22861 ttcgagtggc tcgctaaagg ctattctatc gccccctctc cgggggcgat ttcagatcag
    22921 gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg ttctaccgta
    22981 tctaccactg cctgagtttg tggatcgatt tcaatgttga cgcgtgcgcc aagttttttc
    23041 ttcccaagag tcgtgcgttc cagtgtttcc ggaattaaat ggacgcaaaa acgcgttggc
    23101 gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt gtacagaata
    23161 tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt ttccgaggtt
    23221 aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg tccgccaatt
    23281 tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt taaatcgcca
    23341 agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt gccgttaatt
    23401 tccgtcacgg tcaggcagca accgttatgc gccacggacg caccggtttc caggccgtcc
    23461 agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc gtcaatcgac
    23521 accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc tgaaatcagt
    23581 taagacattc tgttcagcac aatagcaggt ggcaaacgcc cttaccagtg aaggggtaag
    23641 aatgactatt ttttcactgg agaattaata aatcctcgct acaatagact gaatttcccc
    23701 tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata actacaaata
    23761 aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc attagcaatc
    23821 ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac cgtgatggcg
    23881 ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat ctggcttccg
    23941 gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc gcaattaaat
    24001 ggttctggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg gctggcaggt
    24061 tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat ccgctccatg
    24121 gaaaacatcg atccggctct ggcggagaaa gccgtgggtt atctgcgtgc gttgctgtgg
    24181 ggcgcgccgg gatatctgtt cttccaggtt gcccgtaacc tgtgtgaagg tctggcaaaa
    24241 accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacattcc ggtgaactat
    24301 atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg tggcgtggct
    24361 actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa acgcgcccgc
    24421 tccatgcgcg atattcgtaa cgaaaaaggc accgcgaaac ccgatcctgc ggttatgaaa
    24481 cgactgattc aactcggttt gccgattgcg ctggcactat tctttgaagt gacactgttt
    24541 gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg acaccagatt
    24601 gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc agcggtaact
    24661 atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac cgctgcgcgg
    24721 accgggctta tggtgggtgt ctgtatggca accctgacgg ccatattcac ggtttcactg
    24781 cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct ggctgcgcat
    24841 ttgatgttgc tggcggcggt atatcagatt tctgactcga tccaggtgat tggcagtggg
    24901 attttgcgtg gttataaaga tacgcgttcc attttctata ttacctttac ggcttactgg
    24961 gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt tgaacctatg
    25021 gggccagcag gcttctggat aggctttatt attggcctga cgtcggcagc cattatgatg
    25081 atgctgcgta tgcggttcct gcaacgtctg ccgtcagcca tcattctgca acgagcgtcc
    25141 cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac gcaggctgga
    25201 gcgttgcgcc aatcccgtct tcgtctggct gtagtttcag tgcgttacac agagtttgcc
    25261 gactggcatc tgttatctca acgcgtatat ccccggtaac ttcccctttc acattgatga
    25321 agtcaggtga aatttctttc gcactggttt gcaggcaaat cgctccctct ccgcgcaggc
    25381 gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt tttccgccat
    25441 tcagattgat gttaccaaca atgaaagtga cactgtcacc ttttaccgta ctgccgtcga
    25501 gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagctct gcgccataac
    25561 caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca accggtgcgc
    25621 gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg atcactgttc
    25681 caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccga ccacccacat
    25741 tcacggacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc gtccccagac
    25801 gataaatgga tccattctca acaatagtgt cagagtcttc accaccgaga ttattcatgg
    25861 tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg ccgccctgat
    25921 taatctcaca attgctggct tccccacctg gatacaccaa caattcacca ccttcatcta
    25981 ccacgaccgc tgaggttgtc ccattgacca acaggccgcc ctcttgatcg agaatgattt
    26041 tttccgcgcg atgtccttca agtacacgca ggttaccgcc attttccagc aacaaaccgc
    26101 aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta accgtagcga
    26161 gcgtagagag actaattgcg cctccctcgg actgaacaac atctgtggca atggcaccgt
    26221 cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttgacgatc ccaccggttg
    26281 caactttcaa ccagccggtc cccgtgacca cggtattttc agctcttccg ccagcctcaa
    26341 caattaactc gccttcagta agttgagtcg cgccaatgac ctcgccggtc ttaacaattt
    26401 gcacgttccc gtcgctcatc aagtttttcg catcagatcc catgatttat tcctttgctg
    26461 catctgtgtg cctttattgc tacctaagtg taaaggctac ggaggattta tccacgacag
    26521 atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat tgggttgtat
    26581 cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa tatagccaat
    26641 ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt gcctcttgcc
    26701 accttcccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt gcgttcatag
    26761 ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag tccaattgaa
    26821 cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat ggtgggggtc
    26881 ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtcttct cctttttccc
    26941 tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt atgcgttatc
    27001 agaaggagaa tcgctatggc aactttgtta caacttcatt tttctttcaa tggcccattt
    27061 ggcgacacaa tggctgagca gcttaaaccg cttgctgagt cgattaatca ggaacctggt
    27121 tttctgtgga aggtatggac cgaaagtgaa aagaaccacg aagccggtgg gatctacctt
    27181 ttcactgatg aaaaaagcgc ccttgcctat ctggaaaaac acactgcccg actgaaaaat
    27241 ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact gagtcaaatc
    27301 aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc ttttcatccc
    27361 tgtctgaaat ttctcaaatt ctaaaaattt caaccaaact tatctgataa cactaaattc
    27421 gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt gccgggccta
    27481 cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct atttctatct
    27541 tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa aactcaaaaa
    27601 tgatgctggc aaatattgcc agtattgaaa taccgccgat ttattgtacg tatctcgaat
    27661 ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa gaaaccttgc
    27721 acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat caatttttac
    27781 gattagtaga ccaggcacga aagcaaaaat ttgcagtggc tgtttatgaa tcatgccagg
    27841 ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag gatttaccca
    27901 gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat gaagaagaag
    27961 caacccgaac gtattttccc agcccgtggt caggcctgat ggaagcaaag gtcgatgcgt
    28021 gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg gcagtggcta
    28081 ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttcac cgcgataacg
    28141 caagtgaaaa gctaaatatc acgttgatgt cgcgcacggg tattttaccc gaagccgatt
    28201 tctattgccc tattccctac gagcccttac acatcgtcac cgatcaggca ttaaatgctg
    28261 agattcaaaa aggcgaagag ggccttttgg atcgggtatt tagattgata gtagaggaaa
    28321 tcaagtttgc tgatccagac tggagtcaac gcatagcctt agagagcctg aatgtcgatt
    28381 cctttgctca agcctggttt gccgagcgca aacaacgcga cccatttgac tgggcagaaa
    28441 aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc tggcgttatg
    28501 tcattctgcg cctgcatgaa gccgtacagg aaattgttcc acatctgaat gaacacgacc
    28561 ataaacggtt cagtaaaggc cttgcccggg ttttcatcga taattatgcg gcaatccctt
    28621 cagagtctat tcgtcgccta cttgccttac gtgaagcggg aatcattcat attctcgccc
    28681 tcggtgaaga ctacaaaatg gaaattaatg agtcgcgcac cgtcctgaaa acggaagaca
    28741 acagctactc gtttgacgtt tttatcgatg cccgcgggca gcgtccgctt aaagtgaaag
    28801 atattccttt ccatggacta cgcgaacaat tacagaaaac aggggatgaa atccctgatg
    28861 ttggtgaaga ttatacgtta cagcaacccg aagatattcg tgggcgcgta gcgttcggcg
    28921 cgttgccctg gttaatgcac gaccagcctt tcgttcaggg acttacggca tgtgcagaaa
    28981 ttggtgaggc gatggctcgg gcggtcgtaa aacctgcctc ccgtgcacgt cggcgtcttt
    29041 cgtttgatta aaggtaaagc tatcagcact caacaaactg atgctgttca ttaagacgat
    29101 agcggactcc ggctcgaata ttgttttcac cttcaatggc gacggcgaag cgaactcttt
    29161 cgccgtcatg atataccagt gcggcgcttc ctcctggccc gagaataatt gagtcgacca
    29221 cgcccgtgct cactaccgtt gaattttcac cgctggcaat gatgtgtaca ttgtcgccac
    29281 tgttggcaat tctggcgttt gcgccaaagc tggcgatttg caccaggtca ccattactgg
    29341 cgacatgaca tcgttcaccc aatgtacaaa cgcgtactcg cattccggta ttcgcaatcc
    29401 gactgctatc tccagcgctg cttatccgcg aagaattacc ggttacagca acccgtgcgc
    29461 gttcaccgga gctgccaatg tgactgttaa agccgacact tccaatccgc acgctataac
    29521 ccatactggc aatttgcgcg gcatacccag cggttgcgat tctggcgtta tcctcacaac
    29581 agccgatgcg agcatgatcg tcgctgcggg caaactgact gcaaaatggt ctctctccgg
    29641 ggtctgtggc cagtttttgc atagaggaaa cttcctgatg cgcgaagttt tcatccgcca
    29701 gccatttttt ccacgcatac tcaaccagac tctccgccca gtccgtatat ccttcctcaa
    29761 tcagtgcatg atgaatatcg gcataactac cgccgcgtgg gaaatggcgc aggaaccagc
    29821 gatacatcac cgctccgata cgccattctc gcaggtctgc ttgtgtaatg atcattcgtt
    29881 agtgccggtg atagccatcg accatgcttt taaaggtttc gtgtggcgta cgccccgtgg
    29941 tgcaaagata aagatgaccg aagataaaaa agaggcttat aaatgccaga gcaaaatgtg
    30001 tctgcaataa ccagtatctt acgccaggaa acacatctcc cacggcttgc ggatagagac
    30061 acagcagccc cgtcaatagt aacaacggca gcaatccata catgacacca acataggcaa
    30121 cctgctgtaa gggattaaat ttagactggg ttgttgccgg gaaaggatgt tcttccccct
    30181 gcataatgcc aaacaaataa aatcgcgttt gttttgccgc ccgctccagc caaccctgac
    30241 gacgaatgcg atagtggtgg ccattacccc caacggcatt gatcagaaca aagccgagcc
    30301 agcatgccag taacaaaaat ccgcaaactt catgcaccgc aaccagactt ttaaccgcag
    30361 ttgcgcccac cagcgcaaag tgatttatca gcccacttgc cagcaacaat acaaagagta
    30421 acgcattcga ccagtgccat aatctgaccg ctttgctgta aagataaatc ttctcaccat
    30481 gatcggtggc tgatttcttc acgccacgag cacgaagcaa ggcgtgcagc cccagcacca
    30541 accacatccc aaccagcaat actccggcaa tgatcaacca caccggccaa aattcgggag
    30601 tgaactgtgg cacatagttc gctaactggc tctgaaactg ttcagcatgt tgcgacgggt
    30661 tcatacattt tcctttttaa tcaaatgttg accgaaccgg cgatacagat gcggtttccc
    30721 ggcaccaggt agctgatatt gatagtattt attatcctgc aaccacgcct gaatttctgg
    30781 gctatcttcg cgaccaaaga tcaatgcgtg ttccgggcag gcgctgacgc aaatgggcgg
    30841 aaagcctttt gccagccggg actcagcgca gaaatcacat ttatctgcca ctttagtgac
    30901 agggttaagg tagcgaacct ggtacggaca agcgccaatg caataactgc agccgatgca
    30961 ctgcgatttt tctacccgca cgatcccctg ctcatcacgc cacgatgccc ccgtcgggca
    31021 aacgtcgatg cacggagcat cttcgcagtg ctgacatgac tggcgaaaaa agtgatactg
    31081 cgtttcgttg tcgttatcgg tgactggaat gtgtgctatc gataaacggc ttccctgagc
    31141 ggggacgtgg ttagttttac ggcatgcgcg ggcgcaaata ttacagccat tgcaccgtga
    31201 ctcgtcatga atcatggcat accgaacttc cttttcttgc ctcgtgttcg ctaacagaga
    31261 agacgcagaa ccagtaaaaa agattactgt tcccatcccc agaacaaatt tgcgtcgagt
    31321 gaaggacatc tgattatccc ctaatgcagt ggctgcaact gtccacccta tccatgacat
    31381 ctccctgaaa tttcaggggt tctgaaactg ctctacctcg aaccattcgc ataagccata
    31441 agccttgcca taggtcataa atcgctcgct aaacaagtac aacaccgccg tcgatgactc
    31501 cacctgccgt atatcggcat attcaggtgc aacatccatc gcggcgatcg cggcttcaat
    31561 ctgcgcttcc tggaagtaat atggcgcttg catcagcatg gcgactttgt aaggacgagg
    31621 atacgtctgg cactcaaaac gaacggcgtg tgcaatggcg cgacaaatgt cctgctccac
    31681 aacttgcaaa gacatcgccg cgtagttttc actcatggcc tgcgttgagt aatagtaatc
    31741 atccttgctt cccgtcaaac aagcgatatc cgcgtagtct ccgttctgct taatgctttc
    31801 cagcaaaact gacagttcct cttctgcaac caaatagggt ggcgagagaa acacggcgcg
    31861 cgccaccagc tgcccagccg cagaatgctg gcgaatgaac tcagccagga tttcagcagg
    31921 ttctggttcg gttaccgtat caacggggtg acgcatcccc tgcaaccatt gccgcttcgc
    31981 ctcgtcaact tcgctgactt tcgctaacgg caattcatcc tgatggttca tcttacccat
    32041 tccttacgca ggcagtagat tgtgtgctgc cagatcggcg gcaatatctt ccagccccag
    32101 acgctgcaat gtttcgcggg ttggacaacc aagctgtggg tcccagccca tttctttgta
    32161 gaacatcgtg agcgaagcat gcatatcgtc acgatccatt ttgtcagtac cttcagtaaa
    32221 gaccgggatc tgcggatcct tgtcgaatac ccaggaacag ataagatcgt gttcattacg
    32281 catatctttg gtttgcatca gttttaccgt gtaggcacga tgcaacgtaa aaatacgctc
    32341 tgcagctaaa tctaattttt cctgagtcat ctcttcgccg gtgatcgctt tgaagaattt
    32401 ggcttcaagc gccagatcgc cccggtaatt acggcttttc agcggcgaaa cggtcattgg
    32461 ccagacccag ttgcacaggg tgacggcgtt atgcaaacag acccgcaaca gcgaccactt
    32521 tgcatattta atttttgcgt cattgattgg cgtgtagttt ttggtttcat cgtaagcatc
    32581 ttcagaacca aaaagttctt tcgccacttc acgttgcagt ttcaatggca agccggaacc
    32641 aataaagttg atatgggtat gcgtcatgca gtcacggttg aacatacagt taacaatgga
    32701 accgacctgc gctgacgctt cattggcatg gtgaaccgga tagccaaacg gcgaccagag
    32761 tttatttttc gcgtagcccc agtactcttc acccaaattc cagcgttctg cgatggcata
    32821 tgaaccatca gccaggtgac tcagctcacc cacacgatgc gccagacggt agtaaaaatc
    32881 tttaatgaag ttaacgtcac ccgcttccag ttgatcccag cgaatttctg catactcttc
    32941 agctggcaga acacgcttga acacaccttt gctgtaacaa taagtaaagt cgcgatgcaa
    33001 ctgcccgtag ttacaccata ggccgtagtc atcgaacaaa ttcagaccca ccaggttacc
    33061 aatcacacga ccatcgtctt tatcttcaaa atctttcggc ccgttcggga agatggtggt
    33121 atggacaaag tttgctacac aagtgttacc acctgtgctg ggcacgccaa actctttcac
    33181 ccgaggaata ttcatttggg tcatacaacg gatcgggcaa gagtggcagc cgctcatttt
    33241 tactgtgtat ttctctgccg ccggtcctaa gtcaaaaacg gatttatagg tacgaaagcc
    33301 gaccgtattc tgattgcctg gcggaatttc acccgtttca atcggaccac cttcagccgc
    33361 gccccaaaac agccctttac gtgctgtcca gcgtgacttg ggatctgaat actctgccca
    33421 cgattgtggc gtacttggca cgacatggtt gttattcgca ccaataagtt cagtcatcat
    33481 gtaatcattg agacgcttca tctcctgacg atcggcaatg ttgacccctt tcgtcccttc
    33541 aaccgcaatc gctttcaggt ttttcgaacc cattattgcg ccagttcccg caccgccgct
    33601 gtggttacgg ctattcaaca tgccagagag aggaacaagg ttttccccag cctgaccaat
    33661 agccgccaca caggtttccg gactggtcaa tcgacaaatt tcttccgtcg ttgcgcgcgt
    33721 cccttttccc cataagaaat cggctttttc caggctaact ttgtcatctt taatcttcag
    33781 ccataccggt gatttcgcct tcccttcgat aataatgacg tcgtatccag cgaatttcat
    33841 ttgcgctgca aaaaagccac ccatatgggc atcgacgact aaatttcctt tggtaaaagt
    33901 agaaagtgag gtgatattta cgcgagaact acagggggca ccagatccag ttaatgggcc
    33961 ggtagcaaag actaatttat tcgcttcatc gaaaggtttc gtgcctggcg gtacttcgtc
    34021 atacataatt ttgtagccga agcccatgcc accgacaaaa cttttaaact tactggaatc
    34081 ttcgagggta atatttcctg tcgtgagatt gactcttaat atattacctg tccaaccgtt
    34141 agccatgatt ttttcctttg caagattcac acagtaatat ctttccactc gataattttt
    34201 aacgcccctg ttgggcaggc gtttgcgcat tcaccgcata acacacattt cgaggatttt
    34261 ttactttcgg tatttacggt ggccatcatc cacgggcatg ccgtagtaca ggcgctacag
    34321 ccaatacagc gtttatgatc gacggtaata cagccttctt tctgctgcca ggtaatcgca
    34381 ccaatcgggc agacgttcat gcattgcggc tctttgcatt gacggcaggt gtccgcggta
    34441 tagttgagat cgccatacag gccgccgcca gagccaaccc cgttgtcgcc aaagaaataa
    34501 ttgcgatgga ttttgatacg ggagaagaat gtccctactg agccatcgtt gaagttggta
    34561 caagagattt cacagcggtg gcaacccgtg catcgcgctc gctgggtcac cagcacccct
    34621 ttgggggtat ttatcaaccc tactgtgccg ctatcaatat cttcctgttt gcaacccaaa
    34681 agtgacagta acgcaggggc aattgttaga ccagcaaggc cttttcctga tatacgcaag
    34741 aattctaatc gcgttaagcc aatatctaat agtggacgat caaccgggtt catttatttc
    34801 gcatcctctt cgcaaacaca gtgctgacaa ttcagcagcc tgatattaag cattcaacta
    34861 atacgctgct gactctgtcc ccaacaccag atattttccc taacccgata gtgtatgaga
    34921 aaagattaac gccgataaac taatccttta tggttaggag aaaaaataat gtgattattc
    34981 tgagccctta acattgatcg ttatcaatta aaattacaaa gcaagaaatt ataagtgaac
    35041 tgatatttat tatcatttga aataaattta acttaagcga caattatttg tgacgttcat
    35101 tgcagaaaat aaaaataaca ttatcgctat aaattaaaat acataccgaa attcgtataa
    35161 tttcagcagt tgttatttag aatgattaac tggaccgcaa ctgaagcaag aatatcagga
    35221 tgaagcttac cgcctcatcc tgaatggtca ttttaccagt gcgttaatga cggtttccat
    35281 cgcctgcttt tccagctcac tgcgctgaat gcgtttattg gctaatgctg tacgtaatac
    35341 gcctgcgata acaatatgct ttggttcctg ccccagatcg cgcatttcca gcacaacttt
    35401 accgacaact ctgcacattt ctcggtacag ttcatcctct tttgtccgat ttcccattct
    35461 ttatacctat ttatcatgcc aactatcagc atatatcaat ctaaccaatt aaacaaaaat
    35521 caaacaaaat cagacaaata acgcgataaa ttattttaac tgtagcaatt gagcgatgat
    35581 atatttatac accggatgaa ctttcactta tcctcacact gacaacttcg gcaccagacg
    35641 ttgcgcaaac agtgaagttt ttgcgtaacc ttttccctgg aacgttaaat ctttgataac
    35701 aatttattgt ctaacaagtt gtatattttt tgaaacgctg tttttgtttt ccttttggat
    35761 taatttcagc gtataatgcg cgccaattga ctcttgaatg gtttcagcac tttggactgt
    35821 agaactcaac gactcaaaaa caggcactca cgttgggctg agacacaagc acacattcct
    35881 ctgcacgctt tttcgatgtc acctatcctt agagcgaggc accaccactt tcgtaatacc
    35941 ggattcgctt tccggcagtg cgcccagaaa gcaagtttct cccatccttc tcaacttaaa
    36001 gactaagact gtcatgaaaa agaccaaaat tgtttgcacc atcggaccga aaaccgaatc
    36061 tgaagagatg ttagctaaaa tgctggacgc tggcatgaac gttatgcgtc tgaacttctc
    36121 tcatggtgac tatgcagaac acggtcagcg cattcagaat ctgcgcaacg tgatgagcaa
    36181 aactggtaaa accgccgcta tcctgcttga taccaaaggt ccggaaatcc gcaccatgaa
    36241 actggaaggc ggtaacgacg tttctctgaa agctggtcag acctttactt tcaccactga
    36301 taaatctgtt atcggcaaca gcgaaatggt tgcggtaacg tatgaaggtt tcactactga
    36361 cctgtctgtt ggcaacaccg tactggttga cgatggtctg atcggtatgg aagttaccgc
    36421 cattgaaggt aacaaagtta tctgtaaagt gctgaacaac ggtgacctgg gcgaaaacaa
    36481 aggtgtgaac ctgcctggcg tttccattgc tctgccagca ctggctgaaa aagacaaaca
    36541 ggacctgatc tttggttgcg aacaaggcgt agactttgtt gctgcttcct ttattcgtaa
    36601 gcgttctgac gttatcgaaa tccgtgagca cctgaaagcg cacggcggcg aaaacatcca
    36661 catcatctcc aaaatcgaaa accaggaagg cctcaacaac ttcgacgaaa tcctcgaagc
    36721 ctctgacggc atcatggttg cgcgtggcga cctgggtgta gaaatcccgg tagaagaagt
    36781 tatcttcgcc cagaagatga tgatcgaaaa atgtatccgt gcacgtaaag tcgttatcac
    36841 tgcgacccag atgctggatt ccatgatcaa aaacccacgc ccgactcgcg cagaagccgg
    36901 tgacgttgca aacgccatcc tcgacggtac tgacgcagtg atgctgtctg gtgaatccgc
    36961 aaaaggtaaa tacccgctgg aagcggtttc tatcatggcg accatctgcg aacgtaccga
    37021 ccgcgtgatg aacagccgtc tcgagttcaa caatgacaac cgtaaactgc gcattaccga
    37081 agcggtatgc cgtggtgccg ttgaaactgc tgaaaaactg gatgctccgc tgatcgtggt
    37141 tgctactcag ggcggtaaat ctgctcgcgc agtacgtaaa tacttcccgg atgccaccat
    37201 cctggcactg accaccaacg aaaaaacggc tcatcagttg gtactgagca aaggcgttgt
    37261 gccgcagctt gttaaagaga tcacttctac tgatgatttc taccgtctgg gtaaagaact
    37321 ggctctgcag agcggtctgg cacacaaagg tgacgttgta gttatggttt ctggtgcact
    37381 ggtaccgagc ggcactacta acaccgcatc tgttcacgtc ctgtaatatt gcttttgtga
    37441 attaatttgt atatcgaagc gccctgatgg gcgctttttt tatttaatcg ataaccagaa
    37501 gcaataaaaa atcaaatcgg atttcactat ataatctcac tttatctaag atgaatccga
    37561 tggaagcatc ctgttttctc tcaatttttt tatctaaaac ccagcgttcg atgcttcttt
    37621 gagcgaacga tcaaaaataa gtgccttccc atcaaaaaaa tattctcaac ataaaaaact
    37681 ttgtgtaata cttgtaacgc tacatggaga ttaactcaat ctagagggta ttaataatga
    37741 aagctactaa actggtactg ggcgcggtaa tcctgggttc tactctgctg gcaggttgct
    37801 ccagcaacgc taaaatcgat cagctgtctt ctgacgttca gactctgaac gctaaagttg
    37861 accagctgag caacgacgtg aacgcaatgc gttccgacgt tcaggctgct aaagatgacg
    37921 cagctcgtgc taaccagcgt ctggacaaca tggctactaa ataccgcaag taatagtacc
    37981 tgtgaagtga aaaatggcgc acattgtgcg ccattttttt tgtctgccgt ttaccgctac
    38041 tgcgtcacgc gtaacatatt cccttgctct ggttcaccat tctgcgctga ctctactgaa
    38101 ggcgcattgc tggctgcggg agttgctcca ctgctcaccg aaaccggata ccctgcccga
    38161 cgatacaacg ctttatcgac taacttctga tctacagcct tattgtcttt aaattgcgta
    38221 aagcctgctg gcagtgtgta tggcattgtc tgaacgttct gctgttcttc tgccgatagt
    38281 ggtcgatgta cttcaacata acgcatcccg ttaggctcca cggaatattt caccggttcg
    38341 ttgatcactt tcaccggcgt tcccgtccgc acgctggaga acaaggcttt aatatccggt
    38401 gcattcatgc gaatacaccc tgaactgacg cgcaaaccga cgctgtccgg cgcactggta
    38461 ccatgaatga ggtattcgcc attaccatgc gcgaggcgca gtgcgtaacg tcctagcggg
    38521 ttatttggtc cggcaggaac gactggcggt aatttaatgc cacgctccag cgaacgctga
    38581 cgaatgcctg ccgtaggcgt ccaggttggg ttagggattt tctgcccaac acgcgtttcc
    38641 atcaccggcg tttccagccc ctgcaatcca atacctattg gataaacctg cacaatattt
    38701 tctcccggcg gataataata aaggcgcagc tctgcaaggt taacgataat cccctgacgc
    38761 ggtgcatcag gtaataacag ttgtgaagga atagttatcg tcgtaccagg ttttggcacc
    38821 ggggcgatag tgttattggc ttcaaggatc aacattgccg cagtatcaaa acgtcgggca
    38881 atagcctgaa ggtttttatc cccttcttgc accgtatacg tttgattttg cccaaccagt
    38941 cggcttccgg ttggtggtag cggataatca accgcccagg cagcctggat ggcgctaaaa
    39001 gcgccgataa gcgtgagtgt aagcaaagac gcgcgtttca ttgtaaacct cctgtatttg
    39061 ccggagactc acgctgaaac gtcggatggc gcttatgttc acctgaaacc aaaacactcc
    39121 tgtgcaggtc agtgtaaaca ttgaccatcc ggcaatgtga gccaaccgga tgaaagctgt
    39181 ccttttagtt tagctaagtg cagcggcttt ggcgcgaatt gcgcgaatca tcgcttccag
    39241 accttgtgaa cgagatgggg tgagatgttg ggtgagcgcc attttttcaa accacggacg
    39301 cacatcgaaa ttgacaatat cctgcggcgt catctgatcg tagagaataa agacgaccgc
    39361 aataagccct ttcacaatcg ccgcatcgct gtcgccctgt aattcaataa ttccctgggc
    39421 attctggcgc atgacaatcc acacctgact ctgacagccc tgaatgctat tttgtggact
    39481 tctgtcttcg tcgcgtaatt ctggcagacg ctggcccagc tcaataatgt agagatattt
    39541 ctcttcccag ttggcgcagc gtaaaaaatt acgcagcaac ttttctttat ccggcaataa
    39601 agccatagtg cctccctgtt atcccagcaa acggtgaata cgttgcaggc cggtcaccag
    39661 acgatccact tcttcatggg tgttatacat ggccagcgac gcccgacaca tcgcagggac
    39721 gttgtaatag gccatcaatg gcattgcgca gtgatgtccg gtacgcacag caatgccgta
    39781 attatcgaga aaactgccaa catcataggc gtggtgttta ccgagattaa aagcaataac
    39841 gccaagcctg ttttgtgggc catagagagt gagatccggt acagattcca gctgtgatag
    39901 cgcataatgc atcagattct gttcatactc ggctatgtta ttaagcccca gcgccgaaac
    39961 atactccagc gccgcgccaa gaccaatgat gcccccggta ttgggtgtac cggcttcaaa
    40021 ccgccatggt gctttggtcc aggtagtgcc ttcactcagg ctgacggtgg cgatcataga
    40081 accgccccct tcccacggcg gcatctcctg caacaaggct tctttgacat aaagaatgcc
    40141 aattccggtg gggccataca gtttatgccc ggagaacacg taaaagtcgc aatccagcgc
    40201 ctgaacatcc accagatgat gcatcaccgc ctgagcgcca tccaccagca cttttgcgcc
    40261 atgctggtgc gcaagcgtga tcatttccgc cagtggattt tctgtgccaa gcacgttgga
    40321 gacatgagta attgccagca ggcgagtttt ctcatcaaac agcgtaggca gcgtctccag
    40381 ttgcaacgta ccgtcgggat tgagcgggat cacacgcagc tctgcgccaa cgcgtgcgca
    40441 aagcatctgc cagggaacaa tgttagcgtg gtgctccatc tgacttatga tgatgttatc
    40501 gcccgcccgc acgttgctgt tgccccagct attggcgacc agattgatcc cttccgtcgt
    40561 gccgcggacg aacaccagct cttccgccga acgggcgttg ataaacaacg atgcctgctt
    40621 gcgtacgttc tccattttct cggtcgcctg ggcgcttaag gtatgaatac cgcgatgcac
    40681 cgccgcgtag ccgtgacgat aaaactcggc ctcggcgtca atcacctggc ccggtttctg
    40741 cgcactggcg gcgctgtcga gataagccag cggcaaaccg tttacctcac gagaaagcac
    40801 cggaaagtcg gcccgcactt tgtcgacgga aaaagtcatc ttacacctcc tggcagtcgt
    40861 tgaccgattc gggccagcac ctgctgttta agcccctcat cacgcagtgc ttccgtcagt
    40921 tcagcagcaa aggcgtaaat gatcatctgc tgggcatcct gctgattgat cccgcgcgag
    40981 cgcagataga acatctgttc atcatcaata cgccccaccg tcgcgccgtg gctgcatttc
    41041 acatcatctg catagatttc cagctgcggt ttcgtatcca cttccgccag tttgcccatc
    41101 agcagattat tgttggtcat ctgaccatcc gttttgatgg cgtgctgcgc gacgttgatc
    41161 aaaccgttaa ataccgcgcg gcctttgtcg ctgacgatag ttttgtgcaa ctgtcggctg
    41221 ttacaaaaac ctttattgtg ttccagccag gtgcgggtat cacacacctc gtttttcacc
    41281 ggcatcgcca ggctattgat ccgcagcgtg ctgttttcgc cattgagttg cgtactggtg
    41341 ttgtgtcgta acactgcgcc acccagcagg aaactatggc taaatgcggt ggcatcgtca
    41401 gccagcaaca aatcgttatg ggcaaagtgg tgactgaccg ggttttcaaa cgccagcttg
    41461 atatgctgca agtgggcgtt cgctgcgaca ttgatagtga accgtgcgcc ggtgaagtga
    41521 cgagcatcat taaggctgac aaaatgttcg atcaccgttg cttcggcacc ttccgccaga
    41581 tccagatgat gtcggtaatg ggcagtgttg acctcttcac ctgccacgcc ctgggtaata
    41641 tgcattaaca gcaatggctt tgccggccgt tgaccgcgct tcacggcgat atgcgtcacg
    41701 ctttgtgcca ggctttccgt caaatgcaga aacacttccg cctgaatagc gtcgggtacc
    41761 ccctgacggt cgtcgttaat gctcacttca tacccgctgc cttcagtcgc atcgctcagt
    41821 gctgacacgt aacgtccatc gacaaaaacc agccgcacag cgtctaatgt taacgctaag
    41881 gcatcacgct gctgtgggga tatctctccc gcaatgctga caaactggct attggtcagc
    41941 ccttccagcg gcgtatattt ccagttttca tgtttacgtg tcggcagtcc ggtacgcagc
    42001 aattgttgta aatgctgctg tgcttgcgga gagcgtttcg ctccgtcagc ttcaaacaag
    42061 tgatgccact gttgcagcgc gttactgctg ttcggtaagc cagccataac cctgctcctc
    42121 cagttgtttg accaacgtga aatcgccgga tttcacaatt cgtccctgat acagcacatg
    42181 gacgtaatca ggcttgatgt agtcgagaat gcgttggtag tgcgtaacaa tgatgaatga
    42241 acgcttgccg tcacgcagcg agttcacgcc atcggcaacc acttttaatg cgtcaatatc
    42301 cagcccggag tctgactcat caagaatgca taactccggt tccagcaccg ccatttgcaa
    42361 aatatcgttg cgctttttct cgccgccgga aaaaccaacg tttaccgaac gggttaataa
    42421 atcttccggc atcttcagga gagcgatttt ctcttccatc aaatcctgaa aatcaaagcg
    42481 gtcgagcgtt tcctggccgc gatagctgcg caccgcatta agtgccgttt gcaggaaaaa
    42541 ctggttactg acgccgggaa tttccaccgg atactggaag gccataaaga tgccttcgcc
    42601 cgcgcgatct tccggcgaca gcgcaagcaa atctttgcct ttgaactcaa ccgtgccgcc
    42661 cgtcacttca taatcttctc gcccggcaag cgttgccgat aaggtacttt tgcccgaacc
    42721 gtttggcccc ataatggcgt gaacttcgcc gggatgaacg tcgaggctta atccgcgcag
    42781 gatagcttta tcttccacgc tgacgtgtaa atctttaata cttaacatgt ttattcctta
    42841 tccgacgctg tgttcaagac tgatggcgag gagtttttgt gcttcaacgg caaattccaa
    42901 cggcagctcc gagaacacgt ctttgcagaa accgttaaca atcatcgaga tggcgtcttc
    42961 ttcgctgatc ccgcgttgca ggcagtaaaa cagttgatct tcaccaatac gtgatgtcgt
    43021 tgcctcgtgt tccagttgcg cactattgtt acgacactca acatacggga aggtatgcgc
    43081 cccacaatta gcgccaatca gcattgagtc gcactgagtg aaattgcgcg cattggttgc
    43141 cgtcggcatg attttcacta agccgcgata actgttctga ctatgtccgg cagagatccc
    43201 tttcgagata atggtcgatt tggtgttttt accgatgtgg atcatcttgg tgccggtatc
    43261 cgcttgctga tgaccgctgg tcagcgccac tgagtaaaac tcaccaatgg agttatcgcc
    43321 gcgcaaaatg cagctgggat atttccacgt aatcgctgac ccggtttctg attgcgtcca
    43381 tgacattttg ctgttttcgc cttcgcacaa agcacgcttg gtgacgaagt tgagaatacc
    43441 gccggtgttg ttatcgccag ggaaccagtt ttgtaccgtg gaatatttca cctcggcgtt
    43501 tttatggatg atgacttcca ccactgccgc gtgtaactga tagctgtcac gcaccggagc
    43561 ggaacagcct tcaatgtagc tgacgtagct gtcttcgtcg gccaccagaa tggtgcgctc
    43621 aaactgcccg gttttttccg cgttaatgcg aaaataggtg gaaagttcca tagggcagcg
    43681 cacaccttta ggcacataaa taaacgtacc gtcagaggct accgccgcat taagcgcggc
    43741 aaagaagttg tcattccccg gtaccacggt gccgagatat ttacgcacca gttccgggtg
    43801 atcgtggatc gcctcaccaa aggaacagaa aataattccc tgctccgcca gtttttcacg
    43861 ataagtggtg gcaaccgaaa cagagtcgaa aatggcatcc accgccacct ctttgccttc
    43921 ccgcacggga acgcccaact gctcaaacgc cgcctccacc tctttactta aaaaggcgtt
    43981 cgcgccagtt tgctgcaccg cgccgggttc agacgcgcaa gtgtcgtcac aattaccgca
    44041 cgatggtgct gagtagtagc tgtaatcctg ataattcagc ttgtcgtagt gcgctttcag
    44101 ccagtgcggc tcttccatct ccagccatgc gcgataggca ttgagacgaa actccagcat
    44161 ccactccggc tcattacgct tcgccgaaat tgcgcgcacc acctcttcgt ttatcccctt
    44221 tgccagctca tcggtggcta actgggtgaa gaatccttct ttataattca gcgggccgcc
    44281 ggtccaggtt ttgacatcgt cagttgcttc agtattacga gacatagtac cgcctatacc
    44341 ccaaagcttt cgccacagcc acattcattc tgggctttag ggttgtgaaa tttgaatatc
    44401 tgattaagtc cttcacgaac gaaatcgact tccgtgccat caataaacgg catcgcttgc
    44461 agcgggacaa acagcttcgc gccgtcgtgt tcaaacagca gatcgtcttt gtccggctcg
    44521 ctaacactgt cgagcacata gccaaagccc gcgcagcccg tttgcttcac gcctaagcgc
    44581 acgccgacca tacctggctg ctttgccacc agctcgcgga tgtgtatcgc cgctgcgggt
    44641 gtcaacgtta agccttgcca ggcgaaatct tgtgggttaa aggttcctga atgcatgtcc
    44701 atcgatttac ctcacttcat cgctttcagc gtataacagc atgttagtga taatgattat
    44761 cagttcaacc cagcaaacgc aggggcttta ccgtaaaaca tgctttttgc ctgcttttaa
    44821 taagcataga ccctgatgtg tgggttaaca ggcacgctaa gaattaggta tctcattgtt
    44881 agataatggt tattatctaa ggagcattaa ggctgtgatg aaaaagaaaa agttgtattg
    44941 aaaatgacta tttaagagat aggtaaaaaa gtgcagcgtt cagaaataag aaaaccctta
    45001 agtctgtgcg acacaggctt aagggtttct accccatccg gcgcttatct ccggcactct
    45061 cagtggctta gctcttgaag gggcgataag aataatctca taaagctaac ccgccgtttt
    45121 aacacaaact gcgattagta ttatttttga acaatatcag gcggtagata agcagtatta
    45181 agaaggtcat cgaacctgga cggaggttaa tccaggtcga tttggcgaac ttgcggcatt
    45241 aagtcaggat caatgcacgc ccagacgcca ggcaaagtag atttcttctt ttaattcagc
    45301 agaagagaga gtaagcaggt cagcaaattc aagttctagt tgtttcagac gtttgagata
    45361 ttgggcaggt gaaagattgc tctggtcacg gcgtaaaaat tcaatggcca gctgggtggg
    45421 atcaagttga gtagacatag catcctcgct tttagacaag acctgcacag tataccaccg
    45481 tttactgtgc agataatgac caaaagcaat atgcgtcaca cttttctggt gacaacgtca
    45541 caaaatggcg gtcgtcaatc gtgacgaaca gcacaaacgc cctttctcat cgaagatttc
    45601 aatctgccag acctggtgac gcgaaccgag atgcaacggt ttgcatacgc cgcgcacccg
    45661 cccttctcgt gccgagcgga cgtggttagc attgatttcc agaccaacca ctttttgctc
    45721 accttcggta cataaataac cggcaacgga accgatactt tcggccagta ccacggatgc
    45781 tcctccatgc agcaacccga aaggctgctt tgtccgcgag tctactggca ttgtcgcttc
    45841 aagggtgtca tcaccaatat gttcaaagcg aatatccagg aaccccacca tgtttccttc
    45901 acccatagca ttcagtgctt ccagggtgat ttttcgtttc catatcattt aataatctcc
    45961 agtaaagcct gcacaggatg gcgtaccccc gtgccttcaa cccgtttgac ctgactacgg
    46021 caggaatatc cggtcgccag acagcggtta cgcggcagtc gctgcatagc ctgatgccag
    46081 gataactcat agatcccgag cgaattttta tggtttttcg cttcatgtcc gtaagtccct
    46141 gccatgccgc agcaacccac gctgacattt tccagtttcg cgccaaaacg ggcaaatatc
    46201 gcggcccatt gtgctggcgc acccggcaag gcggtaactt cggtacagtg accaaagaaa
    46261 taccatgatt caccgctgac tgtagccact ggctgtgact caagtgcgct tgccagccat
    46321 tcattcgcca gtaagacgtt aaactcgcca cgctcctcgc ccagcgccag tttatattca
    46381 tcgcgataac aaagtaccag cgccggatcg acgcccacca ttggcatacc cagcttcgcc
    46441 atacggttga ggaaatccgc cgtctttttc gccgtcttcg caaaacgatt aagaaaacct
    46501 ttaatatgct gggctttgcc atttggcgaa aatggcagta acacaggctg gaaacctaat
    46561 ttttcgacca gacggacaaa atccgccacc acttgcgcat cgtaatagct ggtaaagggg
    46621 tcctgcacca ccaacactgt gcgcgctttc tgctctgcat tgagagattc aagctgttcc
    46681 agcgtcatgt ttgccgagcg atgccccacc atttgttgtt gtagcgaggg gaccgacagc
    46741 agcggcaaat caaccatgcc gatatgtttt tccgagagtt tgcgcaccag cggctggtta
    46801 atgaagaagt taaaggtttt cggcgcgcgt gccatcagcg gcgcgtagct ctcgaccgta
    46861 gcgacgaggt ggtcgcgcag cgggcgtaaa taacgggtgt gatagagctg cagaaaacga
    46921 gagcgaaact ccggcacatc aattttgatg gggcactggg tcgaacacgc tttacaggcc
    46981 agacagcccg acatcgcctc tttgacttcg tgtgagaagt catattcgcc tttattcgca
    47041 tgccagctat tgcgcgtgcg ggcaattaac gtccgcaaac tgacgccgga ttcaggcagt
    47101 tcttgttcca gtttgagtgg atcaacgccg cgatccgcca acaaacgcag ccattcacgc
    47161 accagcgttg cgcgcccttt cggtgaatga atccggttct gggtgatctt catcgacgga
    47221 cacatcggac tacgggcatc aaagttgaag cataaaccgt tgccgttaca ctccatcgca
    47281 ccgcgccact gctggcgtac cgcaatgggg atctgccgat cgaatgtacc gcgcttcacc
    47341 gcgtccactt tcatcatcgg cgcatcgaga ccttctggcg ggcaaatctt ccctgggttg
    47401 agtcggttat gcgggtcaaa tgccgctttc actttgcgca gttctgcaaa aagttcctca
    47461 ccgaaaaacg ccgggctgta ttcagcgcga aaacctttgc cgtgctcgcc ccacaacaaa
    47521 ccaccgtatt tcgcagtcag cgccaccacg tcatcagaga tttgcttcat caaaatctct
    47581 tgttgaggat cgcacatatc cagcgctgga cggacgtgca agacacctgc gtcgacgtga
    47641 ccgaacatac cgtagcttaa gccgtggctg tcgagcagcg cgcgaaattc agcaatataa
    47701 tccgccaggt gttccggcgg tacgcaggta tcctcagcaa acggaattgg cttagcggca
    47761 cctttggcat tgccaagcag accaacggct tttttgcgca ttgcatagat acgttcaacc
    47821 cccgccagct cacggcacac ctgccagccg atgacacctg cttgatgact ggcgatcagc
    47881 tcatccaacc gcgcacagag tgcatttacc cgctcatcaa tcagcgcctc atcatcacca
    47941 gcaaattcca caatgttcag cccgagcatc tcttggtcag gcacatcggt aatcaactcg
    48001 ctgacggaat gccagacaat atcttcccgc gccagattca gcacttttga gtccaccgtc
    48061 tctaccgaaa gcgcccgcgc ctcaaccata aacggcgcgt tacgcagcgc agagtcaaaa
    48121 gagtcatatt tgacgttcac cagacggcgc actttaggca agcgcgtaat atccagccgc
    48181 gcttcggtaa taaaggccag cgtcccttct gaacccgtca gaatgcgcgt caggtcgaac
    48241 tcggtcatct catcgttaaa gacatgacgc agatcgtaac cggtaagaaa gcggttaagt
    48301 ttggggaagt tgtcgataat taactggcgt tgctgacggc aacgttgata aaccgtgtta
    48361 taaattcgcc cgattgtggt attggattta cccagcgttt ccgacaattc gacgggtaaa
    48421 ggttgcgtat cgagaatatc gccccccaac aacaccgcgc gtacgccaag tacgtgatct
    48481 gacgttttgc catagaccag cgatccctga ccggatgcat cggtattgat catcccaccg
    48541 agcgttgccc ggttgctggt cgaaagttcc ggcgcaaaaa agtagccgaa cggtttcagg
    48601 tactgattga gttgatcttt tatcaccccg gcctcaacgc gcacccagcc ctcttcaggg
    48661 ttaatttcga tgatgcggtt catatggcgg gacatatcaa caataatccc ctggttgagc
    48721 gcctgaccgt tagtgccggt gccgccgccg cggggggtaa agatcagcga tgaatagcgt
    48781 tcctgcgcgg caagacgggc gatcagcgcc acatctgcgg ttgaacgcgg aaataccacc
    48841 gcatcgggga gaagttggta aatactgttg tcggtcgaca ttgtcagacg atcggcataa
    48901 cttgtcgccg tatcgccggt aaaaccttgt tgctccagct cttgcaaaaa attaagcacc
    48961 agttgaacga cgccgggtgc ctgggaaatc tgtggaatca ttatattgac cctttcctgc
    49021 ggtctgtgat gagagattaa tcgtttgcgt gttgcctgag ttgttgtacc acattttttt
    49081 ctaacacgcc catcagaatt aagggcagaa tcggcctgtt aaaaaccgct gaaattgctc
    49141 atcattatgt aggtgagttt cgcgtgttca cgtcgcgtcg acgatttgac gcacaaaaaa
    49201 ggtgaaaagt agttatggta aatgttcgtc agcccaggga tgtcgcacaa attctgcttt
    49261 cggtgctgtt tttagccatc atgattgtgg catgtctgtg gattgttcaa ccctttattc
    49321 tcggctttgc atgggccggt acggtggtta tcgccacctg gccggtattg ttacgtttgc
    49381 aaaagatcat gtttggccgc cgctctctcg ccgttctggt gatgacgctg ttattagtga
    49441 tggtgtttat catccctatt gctttgctgg ttaacagtat cgtcgacggc agcggcccgc
    49501 taattaaagc catttccagc ggtgacatga cgttacccga tctggcgtgg cttaatacca
    49561 ttccggtgat tggcgcgaag ctgtatgcag gctggcacaa cttgctggat atggggggta
    49621 cggcgatcat ggcgaaggtc cgcccttata ttggcaccac caccacctgg ttcgttgggc
    49681 aggcggcgca tatcgggcgc tttatggtgc attgtgcgct gatgcttctc ttcagtgccc
    49741 tgctgtactg gcgcggtgaa caggtggcac aaggcattcg ccattttgca acccgtctgg
    49801 caggcgttcg cggagatgcc gccgtactgc tggcggcaca ggctatccgc gcggtggcgc
    49861 tgggtgtggt ggtgacggcg ttagtacagg cagtgcttgg cggtatcggc ctcgccgtat
    49921 ccggcgtacc ttatgcaact ttgctaacgg tgttaatgat cctctcctgc cttgtccagc
    49981 ttggaccgtt accggtactg attccggcga ttatctggct ctactggacc ggcgatacca
    50041 cctggggaac ggtattgtta gtgtggagcg gtgtggttgg cacgctggat aacgtcatcc
    50101 gcccaatgtt aattcgcatg ggtgccgatt taccgctgat cctgatcctc tctggcgtta
    50161 ttggtggttt gattgctttc gggatgatcg gtctgtttat tggtccggtt ctgttagccg
    50221 tttcctggcg tctgtttgcc gcgtgggtgg aagaggtccc accgccgacg gaccaaccgg
    50281 aagaaattct cgaagaactt ggcgaaatcg agaaaccgaa taagtaattt ctcatcaggc
    50341 ggctctgccg cctgattgtt aaccaccgct aattgatatt tgcttgctct tccccatatt
    50401 ttcctgctta ccgccattca gctggtagta cctgtcgcaa attctttaca gtttttaaac
    50461 taatgagacg aatctgatcg acacaaaaag tccgtatgcc tactattagc tcacggttat
    50521 aaatcaacat attgatttat aagcatggaa atcccctgag tgaaacaacg aattgctgtg
    50581 tgtagtcttt gcccatctcc cacgatgggc ttttttttaa catttttccg cttcgctacc
    50641 tcgcccctca ctctttccaa taatgttgca caagatactg tgactctctt caagccgtca
    50701 gcaggctgat aatgttattt atattttatt gtttatttca tgcagccatt acacgatgaa
    50761 cgcttatgaa ctccaggcac tccgccatat ttttgccatg actattgatg aatgcgcgac
    50821 ctggattgcc cagaccggtg acagtgaaag ctggcgtcaa tgggaaaatg gcaagtgtgc
    50881 cattcctgat tgtgtagtcg agcaactgtt ggctatgcgt cagcaaagaa aaaaacatct
    50941 tcatgccatc atagagaaaa taaataaccg catcggcaac aacaccatgc gttttttccc
    51001 cgacttaacc gcatttcaac gagtctatcc tgatggcaat tttattgact ggaagatcta
    51061 tcaatcggta gccgctgaat tatatgcaca cgatctggag cggctttgtt aatttttcca
    51121 cagaaaggaa ttgtcgttgt tacaacaata atgaatggat actgacacaa catcgctaca
    51181 ctatttaaag cacctttgct aagtagaacc tatgaaaaat ccctattacc ctaccgcact
    51241 tgggttgtat tttaattacc tggtgcatgg tatgggcgtc attttgatga gcctgaatat
    51301 ggcctcgctg gagacacttt ggcagactaa tgccgcgggt gtctcgatag ttatctcatc
    51361 gctgggcatt ggtcgattaa gtgtcttgct ttttgcagga ttattatccg atcgctttgg
    51421 tcgccgccct tttatcatgc tcgggatgtg ctgctatatg gccttctttt ttggcatcct
    51481 gcagaccaat aacatcatta tcgcttatgt ttttggcttt ctggcgggaa tggcaaacag
    51541 ttttctcgat gcaggcactt atcccagttt gatggaagct tttccacgct cacctgggac
    51601 agccaatatt ttaattaaag catttgtttc cagcggacaa tttttattac cgctaatcat
    51661 cagcctgtta gtgtgggctg aattgtggtt cggttggtcc tttatgattg ctgcaggcat
    51721 tatgtttatt aacgctctgt ttttataccg ttgtaagttc ccaccccatc cgggtcgtcg
    51781 ctcacctgtc ataaagaaaa ccaccagctc tacggaacat cgctgttcaa ttatcgattt
    51841 agccagttat accttatatg gctatatctc aatggcaacg ttttatctgg ttagccagtg
    51901 gctggcacag tacggacaat ttgttgcagg catgtcatac actatgtcga tcaaactact
    51961 cagtatctac accgtgggtt cgctgctttg tgtatttatt accgctccac tcattcgtaa
    52021 taccgttcgc ccaacaacat tactgatgct gtacaccttt atctcattta ttgctctgtt
    52081 taccgtctgc ctgcatccca cattttatgt ggtgataata tttgcttttg tcattggttt
    52141 tacctctgct ggaggtgttg tgcaaattgg cctgacgtta atggctgaac gtttccctta
    52201 cgctaaaggt aaagctacag ggatctatta cagtgcgggc agtattgcga cctttactat
    52261 tccgttgatt acggctcatc tgtcccaaag aagtattgcc gatattatgt ggttcgatac
    52321 cgccatcgct gccatcggtt ttttactggc actgtttatc ggcttacgca gccgcaaaaa
    52381 aacgcggcat cactcgctaa aggaaaatgt cgctccgggt gggtaatgca atattctttt
    52441 catgtcatgc aagatcttac ggataaataa ctctttctgc gctaactaag gaaaatcgcg
    52501 atcaaaaaca aactatgaca tgcaatattc ctggaaacat aaactttatg ccatgtaccc
    52561 agggataatc atcttcagta tagtaattat gtaaaccgtc ggagaacaat acgtacggta
    52621 acgaaataat ctttcagcaa ggagctgtga aaatgtctca aaataaggct ttcagcacgc
    52681 catttatcct ggctgttctt tgtatttact tcagctactt cctgcacggc attagtgtta
    52741 ttacgcttgc ccaaaatatg tcatctctgg cggaaaagtt ttccactgac aacgcgggca
    52801 ttgcctactt aatttccggt atcggtttgg ggcgattgat cagtatttta ttcttcggtg
    52861 tgatctccga taagtttggt cgtcgggcgg tgatattaat ggcagtaata atgtatctgc
    52921 tattcttctt tggtattccc gcttgcccga atttaactct cgcctacggt ctggcagtgt
    52981 gcgtaggtat cgctaactca gcgctggata cgggtggcta ccccgcgctc atggaatgct
    53041 ttccgaaagc ctctggttcg gcggtcatac tggttaaagc gatggtgtca ttcgggcaaa
    53101 tgttctaccc aatgctggtg agctatatgt tgctcaataa tatctggtac ggctatgggc
    53161 tgattattcc gggtattctg tttgtactga tcacgctgat gctgttgaaa agcaaattcc
    53221 ccagccagtt ggtggacgcc agcgtagcta atgaattacc gcaaatgaac agcaaaccgt
    53281 tagtctggct ggaaggtgtt tcatcggtac tgttcggtgt agctgcattc tcgacctttt
    53341 atgtgattgt ggtgtggatg cccaaatatg cgatggcttt tgctggtatg tcagaagctg
    53401 aggcattaaa aaccatctct tattacagta tgggctcgtt ggtctgtgtc tttatttttg
    53461 ccgcactact gaaaaaaatg gtccggccca tctgggctaa tgtatttaac tctgcactgg
    53521 caacaataac agcagccatt atctatctgt atccttctcc actggtgtgt aatgccggcg
    53581 cctttgttat cggtttctca gcagctggcg gcattttaca gctcggtgtt tcggtcatgt
    53641 cagagttttt cccaaaaagc aaagccaaag tcaccagtat ttatatgatg atgggtggac
    53701 tggctaactt tgttattccg ctgattaccg gttatctgtc gaacatcggc attcaatata
    53761 tcattgttct cgattttact ttcgcgctgc tggccctgat taccgcaatt attgttttta
    53821 tccgctatta ccgcgttttt attattcctg agaatgatgt gcgttttggc gagcgtaaat
    53881 ttagcacccg gttaaacaca attaagcata gaggttaaag gagttaatta tggatgttac
    53941 cgcaaaatac gaattgattg ggctgatggc ctatcctatc cgccacagtt tatcgcctga
    54001 aatgcagaat aaagccttag aaaaagcggg attgccattt acctatatgg cctttgaagt
    54061 ggataacgat agttttcccg gagcaattga aggattaaaa gccctcaaaa tgcgcggaac
    54121 tggtgtgtcg atgccgaaca aacaactggc gtgtgaatat gttgatgaat taacgccagc
    54181 agccaaactg gtgggtgcca tcaacaccat cgttaatgat gatggctacc tgcgcggcta
    54241 taacaccgac ggcacgggcc atattcgcgc cattaaagag agcggttttg atatcaaagg
    54301 caaaacgatg gtgctgttag gggccggagg tgcctcaacg gcgattggcg cgcagggggc
    54361 aattgaaggt ttaaaagaaa ttaaactctt taaccgtcgg gatgagttct tcgataaagc
    54421 cctcgccttc gcgcagcggg ttaacgaaaa caccgattgt gtcgtcacgg ttaccgatct
    54481 cgccgatcag caagcctttg ctgaagcact ggcttccgcc gacattttaa ccaatggcac
    54541 aaaagtgggt atgaaacccc ttgagaatga atcattggtt tatgatatca gtctgttaca
    54601 tccgggactg ctggtcactg aatgcgtgta taacccgcat atgacgaagt tattgcagca
    54661 ggcacaacaa gctggttgca aaacgattga tggatacggc atgttgttgt ggcaaggggc
    54721 tgaacagttc acgttatgga ctggcaaaga tttccctctg gaatatgtta aacaggtcat
    54781 ggggttcggt gcctgacagg ctgaccgcgt gcagaaaggg taaaaaatga aaaccgtaac
    54841 tgtaaaagat ctcgtcattg gtacgggcgc acctaaaatc atcgtctcgc tgatggcgaa
    54901 agatatcgcc agcgtgaaat ccgaagctct cgcctatagt gaagcggact ttgatattct
    54961 ggaatggcgt gtggaccact ttgccgacct ctccaatgtg gagtctgtca tggcggcggc
    55021 aaaaattctc cgtgaaacca tgccagaaaa accgctgctg tttaccttcc gcagtgccaa
    55081 agaaggcggc gagcaggcga tttccaccga ggcttatatt gctctcaatc gtgcagccat
    55141 cgacagcggc ttggttgata tgatcgatct ggagttattt accggcgatg atcaggttaa
    55201 agaaaccgtg gcctacgccc acgcgcatga tgtgaaagtt gtcatgtcca accatgactt
    55261 ccataaaacg ccggaagccg aagaaatcat tgcccgtctg cgcaaaatgc aatccttcga
    55321 cgccgatatt cctaagattg cgctgatgcc gcaaagtacc agcgatgtgc tgacgttgct
    55381 tgccgcgacc ctggagatgc aggagcagta tgccgatcgt ccaatcatca cgatgtcgat
    55441 ggcaaaaact ggcgtaattt ctcgtctggc tggtgaagta tttgggtcgg cggcaacttt
    55501 tggtgcggta aaaaaagcct ctgcgccagg gcaaatctcg gtaactgatt tacgcacagt
    55561 attaactatt ttacatcagg cataagcaat aatatttcg
//
LOCUS       DAJOSY010000016        52258 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJOSY010000016 DAJOSY010000000
VERSION     DAJOSY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.016"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            252..1346
                     /gene="gcvT"
                     /locus_tag="OHN27_001073"
     CDS             252..1346
                     /gene="gcvT"
                     /locus_tag="OHN27_001073"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCQ2285557.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1370..1759
                     /gene="gcvH"
                     /locus_tag="OHN27_001074"
     CDS             1370..1759
                     /gene="gcvH"
                     /locus_tag="OHN27_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCQ2285558.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATTYEALLEDE"
     gene            1877..4750
                     /gene="gcvP"
                     /locus_tag="OHN27_001075"
     CDS             1877..4750
                     /gene="gcvP"
                     /locus_tag="OHN27_001075"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCQ2285559.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4916..6349)
                     /gene="bglA"
                     /locus_tag="OHN27_001076"
     CDS             complement(4916..6349)
                     /gene="bglA"
                     /locus_tag="OHN27_001076"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCQ2285560.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPEDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLRKGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            6394..6705
                     /locus_tag="OHN27_001077"
     CDS             6394..6705
                     /locus_tag="OHN27_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCQ2285561.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDETESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            6869..7528
                     /locus_tag="OHN27_001078"
     CDS             6869..7528
                     /locus_tag="OHN27_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCQ2285562.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(7724..8704)
                     /gene="ygfZ"
                     /locus_tag="OHN27_001079"
     CDS             complement(7724..8704)
                     /gene="ygfZ"
                     /locus_tag="OHN27_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCQ2285563.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            8947..9213
                     /gene="sdhE"
                     /locus_tag="OHN27_001080"
     CDS             8947..9213
                     /gene="sdhE"
                     /locus_tag="OHN27_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCQ2285564.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9194..9601
                     /locus_tag="OHN27_001081"
     CDS             9194..9601
                     /locus_tag="OHN27_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCQ2285565.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9641..10162)
                     /gene="fldB"
                     /locus_tag="OHN27_001082"
     CDS             complement(9641..10162)
                     /gene="fldB"
                     /locus_tag="OHN27_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCQ2285566.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10274..11170
                     /gene="xerD"
                     /locus_tag="OHN27_001083"
     CDS             10274..11170
                     /gene="xerD"
                     /locus_tag="OHN27_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCQ2285567.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11195..11905
                     /gene="dsbC"
                     /locus_tag="OHN27_001084"
     CDS             11195..11905
                     /gene="dsbC"
                     /locus_tag="OHN27_001084"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCQ2285568.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            11911..13644
                     /gene="recJ"
                     /locus_tag="OHN27_001085"
     CDS             11911..13644
                     /gene="recJ"
                     /locus_tag="OHN27_001085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCQ2285569.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFKLFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDSIWPI"
     gene            13735..14833
                     /gene="prfB"
                     /locus_tag="OHN27_001086"
     CDS             join(13735..13809,13811..14833)
                     /gene="prfB"
                     /locus_tag="OHN27_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCQ2285570.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14843..16360
                     /gene="lysS"
                     /locus_tag="OHN27_001087"
     CDS             14843..16360
                     /gene="lysS"
                     /locus_tag="OHN27_001087"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCQ2285571.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16403..16951)
                     /gene="idi"
                     /locus_tag="OHN27_001088"
     CDS             complement(16403..16951)
                     /gene="idi"
                     /locus_tag="OHN27_001088"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCQ2285572.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17006..17077
                     /locus_tag="OHN27_001089"
     CDS             17006..17077
                     /locus_tag="OHN27_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285573.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17074..17199)
                     /locus_tag="OHN27_001090"
     CDS             complement(17074..17199)
                     /locus_tag="OHN27_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285574.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17201..18649)
                     /gene="uacT"
                     /locus_tag="OHN27_001091"
     CDS             complement(17201..18649)
                     /gene="uacT"
                     /locus_tag="OHN27_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCQ2285575.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            19085..21004
                     /gene="ygfT"
                     /locus_tag="OHN27_001092"
     CDS             19085..21004
                     /gene="ygfT"
                     /locus_tag="OHN27_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCQ2285576.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVTIGNLERYITDTALAMGWCPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21004..21492
                     /locus_tag="OHN27_001093"
     CDS             21004..21492
                     /locus_tag="OHN27_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCQ2285577.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(21528..22895)
                     /gene="ghxQ"
                     /locus_tag="OHN27_001094"
                     /gene_synonym="ygfQ"
     CDS             complement(21528..22895)
                     /gene="ghxQ"
                     /locus_tag="OHN27_001094"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HCQ2285578.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22931..24247)
                     /gene="guaD"
                     /locus_tag="OHN27_001095"
     CDS             complement(22931..24247)
                     /gene="guaD"
                     /locus_tag="OHN27_001095"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HCQ2285579.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24265..25665)
                     /gene="xanQ"
                     /locus_tag="OHN27_001096"
     CDS             complement(24265..25665)
                     /gene="xanQ"
                     /locus_tag="OHN27_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HCQ2285580.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(25830..28700)
                     /locus_tag="OHN27_001097"
     CDS             complement(25830..28700)
                     /locus_tag="OHN27_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCQ2285581.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(28697..29476)
                     /locus_tag="OHN27_001098"
     CDS             complement(28697..29476)
                     /locus_tag="OHN27_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCQ2285582.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(29527..30855)
                     /gene="ssnA"
                     /locus_tag="OHN27_001099"
     CDS             complement(29527..30855)
                     /gene="ssnA"
                     /locus_tag="OHN27_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCQ2285583.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SFKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(30858..33956)
                     /gene="ygfK"
                     /locus_tag="OHN27_001100"
     CDS             complement(30858..33956)
                     /gene="ygfK"
                     /locus_tag="OHN27_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCQ2285584.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTIEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLLVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(34278..34856)
                     /gene="mocA"
                     /locus_tag="OHN27_001101"
     CDS             complement(34278..34856)
                     /gene="mocA"
                     /locus_tag="OHN27_001101"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCQ2285585.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWEQGTTLDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLIQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKERYTEI"
     gene            34959..35729
                     /gene="yqeC"
                     /locus_tag="OHN27_001102"
     CDS             34959..35729
                     /gene="yqeC"
                     /locus_tag="OHN27_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCQ2285586.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            35777..37402
                     /locus_tag="OHN27_001103"
     CDS             35777..37402
                     /locus_tag="OHN27_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCQ2285587.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(37623..38555)
                     /gene="arcC"
                     /locus_tag="OHN27_001104"
     CDS             complement(37623..38555)
                     /gene="arcC"
                     /locus_tag="OHN27_001104"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCQ2285588.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(38603..39988)
                     /gene="hyuA"
                     /locus_tag="OHN27_001105"
     CDS             complement(38603..39988)
                     /gene="hyuA"
                     /locus_tag="OHN27_001105"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCQ2285589.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(40041..41252)
                     /locus_tag="OHN27_001106"
     CDS             complement(40041..41252)
                     /locus_tag="OHN27_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCQ2285590.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(41310..42506)
                     /gene="dpaL"
                     /locus_tag="OHN27_001107"
     CDS             complement(41310..42506)
                     /gene="dpaL"
                     /locus_tag="OHN27_001107"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCQ2285591.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNTIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(42564..43751)
                     /gene="ygeW"
                     /locus_tag="OHN27_001108"
     CDS             complement(42564..43751)
                     /gene="ygeW"
                     /locus_tag="OHN27_001108"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCQ2285592.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            44230..46008
                     /locus_tag="OHN27_001109"
     CDS             44230..46008
                     /locus_tag="OHN27_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCQ2285593.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(46048..46527)
                     /gene="xdhC"
                     /locus_tag="OHN27_001110"
     CDS             complement(46048..46527)
                     /gene="xdhC"
                     /locus_tag="OHN27_001110"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCQ2285594.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(46524..47402)
                     /gene="xdhB"
                     /locus_tag="OHN27_001111"
     CDS             complement(46524..47402)
                     /gene="xdhB"
                     /locus_tag="OHN27_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCQ2285595.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(47413..49710)
                     /gene="xdhA"
                     /locus_tag="OHN27_001112"
     CDS             complement(47413..49710)
                     /gene="xdhA"
                     /locus_tag="OHN27_001112"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCQ2285596.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSQITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            50125..50880
                     /locus_tag="OHN27_001113"
     CDS             50125..50880
                     /locus_tag="OHN27_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCQ2285597.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            50959..51032
                     /locus_tag="OHN27_001114"
     tRNA            50959..51032
                     /locus_tag="OHN27_001114"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50991..50993,aa:Gly,seq:ccc)
     gene            51188..52258
                     /locus_tag="OHN27_001115"
                     /pseudo
     CDS             51188..52258
                     /locus_tag="OHN27_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12825 a        12992 c        13651 g        12790 t
ORIGIN      
        1 tctaatggca tcatctttat atagaaactg gtgtgttttt tggtttttta ttctgtcgcg
       61 attttggcgt tttttaacca taagctaatg tgatgatcaa ttttacctta tggttaacag
      121 tctgtttcgg tggtaagttc aggcaaaaga gaacgattgc gttggggacc gggagtggct
      181 ccgatgctgg gtttcgtggt gataatttca ccatgaaaag ttgtcagccc cgcttattca
      241 atgaggacaa gatggcacaa cagactcctt tgtacgaaca acacacgctt tgcggcgctc
      301 gcatggtgga tttccacggc tggatgatgc cgctgcatta cggttcgcaa atcgacgaac
      361 atcatgcggt acgtaccgat gccggaatgt ttgatgtgtc acatatgacc atcgtcgatc
      421 ttcgcggcag ccgcacccgg gagtttctgc gttatctgct ggcgaacgat gtggcgaagc
      481 tcaccaaaag cggcaaagcc ctttactcgg ggatgttgaa tgcctctggc ggtgtgatag
      541 atgacctcat cgtctactac tttactgaag atttcttccg cctcgttgtt aactccgcca
      601 cccgcgaaaa agacctctcc tggattaccc aacacgctga acctttcggc atcgaaatta
      661 ccgttcgtga tgacctttcc atgattgccg tgcaagggcc gaatgcgcag gcaaaagctg
      721 ccacactgtt taatgacgcc cagcgtcagg cggtggaagg gatgaaaccg ttctttggcg
      781 tgcaggcggg cgatctgttt attgccacca ctggttatac cggtgaagcg ggctatgaaa
      841 ttgcgctgcc caatgaaaaa gcggccgatt tctggcgtgc gctggtggaa gcgggtgtta
      901 agccatgtgg cttgggcgcg cgtgacacgc tgcgtctgga agcgggcatg aatctttatg
      961 gtcaggagat ggacgaaacc atctctcctt tagccgccaa catgggctgg actatcgcct
     1021 gggaaccggc agatcgtgac tttatcggtc gtgaagccct ggaagtgcag cgtgagcatg
     1081 gtacagaaaa actggttggt ctggtgatga ccgaaaaagg cgtgctgcgt aatgaactgc
     1141 cggtacgctt taccgatgcg cagggcaacc agcatgaagg cattatcacc agcggtactt
     1201 tctccccgac gctgggttac agcattgcgc tggcgcgcgt gccggaaggt attggcgaaa
     1261 cggcgattgt gcaaattcgc aaccgtgaaa tgccggttaa agtgacaaaa cctgtttttg
     1321 tgcgtaacgg caaagccgtc gcgtgattta cttttttgga gattgattga tgagcaacgt
     1381 accagcagaa ctgaaataca gcaaagaaca cgaatggctg cgtaaagaag ccgacggcac
     1441 ttacaccgtt ggtattaccg aacatgctca ggagctgtta ggcgatatgg tgtttgttga
     1501 cctgccggaa gtgggcgcaa cggttagcgc gggcgatgac tgcgcggttg ccgaatcagt
     1561 aaaagcggcg tcagacattt atgcgccagt aagcggtgaa atcgtggcgg taaacgacgc
     1621 actgagcgat tccccggaac tggtgaacag cgaaccgtat gcaggtggct ggatctttaa
     1681 aatcaaagcc agcgatgaaa gcgaactgga atcactgctg gatgcgacca catacgaagc
     1741 attgttagaa gacgagtaac ggctttattc ctcttctgcg ggagaggatc agggtgagga
     1801 aatttacgcc tcaccctcac tctcttcgta aggagagagg ttcacaattc actgcacgtt
     1861 tcaggaacca tcgctcatga cacagacgtt aagccagctt gaaaacagcg gcgcttttat
     1921 tgaacgccat atcggaccgg acgccgcgca acagcaagaa atgctgaatg ccgttggcgc
     1981 acaatcgtta aacgcgctga ccggccagat tgtaccgaaa gatattcagc ttgcgacccc
     2041 gccgcaggtt ggcgcaccgg cgaccgaata cgccgcgctg gcggaactca aggctattgc
     2101 cagtcgcaat aaacgcttca cgtcttacat cggcatgggt tacaccgccg tgcagctacc
     2161 gccggttatc ctgcgtaaca tgctggaaaa tccgggctgg tataccgcgt atactccgta
     2221 tcaacctgaa gtctcccagg gccgccttga agcactgctc aacttccagc aggtaacgct
     2281 ggatttaacc gggctggata tggcctctgc ttctcttctg gacgaggcca ccgctgccgc
     2341 cgaagcaatg gcgatggcga aacgcgtcag caaactgaaa aatgccaacc gcttcttcgt
     2401 ggcttccgat gtgcatccgc aaacgctgga tgtggtccgt actcgtgccg aaacctttgg
     2461 ttttgaagtg attgtcgatg acgcgcaaaa agtgctcgac catcaggacg tcttcggcgt
     2521 gctgttacag caggtaggca ctaccggtga aattcacgac tacactgcgc ttattagcga
     2581 actgaaatca cgcaaaattg tggtcagcgt tgccgccgat attatggcgc tggtgctgtt
     2641 aactgcgccg ggtaaacagg gcgcggatat tgtttttggt tcggcgcaac gcttcggcgt
     2701 gccgatgggc tacggtggcc cacacgcggc attctttgcg gcgaaagatg aatacaaacg
     2761 ctcaatgccg ggccgtatta tcggtgtatc gaaagatgca gctggcaaca ccgctctgcg
     2821 catggcgatg cagactcgcg agcaacatat tcgtcgtgag aaagcgaact ccaacatttg
     2881 tacttcccag gtactgctgg caaacatcgc cagcctgtat gccgtttatc acggcccggt
     2941 tggcctgaaa cgtatcgcta accgcattca ccgtctgacc gatatcctgg cggcaggcct
     3001 gcaacaaaaa ggtctgaagc tgcgccatgc gcactatttc gacaccttgt gtgtggaagt
     3061 ggccgacaaa gcgggcgtac tgacgcgtgc cgaagcggct gaaatcaacc tgcgtagcga
     3121 tattctgaac gcggttggga tcacccttga tgaaaccacc acgcgtgaaa acgtaatgca
     3181 gcttttcaac gtgctgctgg gcgataacca cggcctggac atcgacacgc tggacaaaga
     3241 cgtggctcac gacagccgct ctattcaacc tgcgatgctg cgcgacgacg aaatcctcac
     3301 ccatccggtg tttaatcgct accacagcga aaccgaaatg atgcgctata tgcactcgct
     3361 ggagcgtaaa gatctggcgc tgaatcaggc gatgatcccg ctgggttcct gcaccatgaa
     3421 actgaacgcc gccgccgaga tgatcccaat cacctggccg gaatttgccg aactgcaccc
     3481 gttctgcccg ccggagcagg ccgaaggtta tcagcagatg attgcgcagc tggctgactg
     3541 gctggtgaaa ctgaccggtt acgacgccgt ttgtatgcag ccgaactctg gcgcacaggg
     3601 cgaatacgcg ggcctgctgg cgattcgtca ttatcatgaa agccgcaacg aagggcatcg
     3661 cgatatctgc ctgatcccgg cttctgcgca cggaactaac cccgcttctg cacatatggc
     3721 aggaatgcag gtggtggttg tggcgtgtga taaaaacggc aacatcgatc tgactgatct
     3781 gcgcgcgaaa gcggaacagg cgggcgataa cctctcctgt atcatggtga cttatccttc
     3841 tacccacggc gtgtatgaag aaacgatccg tgaagtgtgt gaagtcgtgc atcagttcgg
     3901 cggtcaggtt taccttgatg gcgcgaacat gaacgcccag gttggcatca cctcaccggg
     3961 ctttattggc gcagacgttt cacaccttaa cctgcataaa actttctgca ttccgcacgg
     4021 cggtggtggt ccgggtatgg gaccgatcgg cgtgaaagcg catctggcac cgtttgtacc
     4081 gggtcatagc gtggtgcaaa tcgaaggcat gttaacccgt cagggcgcgg tttctgcggc
     4141 accgttcggt agcgcctcta tcctgccaat cagctggatg tacatccgca tgatgggcgc
     4201 agaagggctg aaaaaagcaa gccaggtggc aattcttaac gccaactata ttgccagccg
     4261 cctgcaggat gccttcccgg tgctgtatac cggtcgcgac ggtcgcgtgg cgcacgaatg
     4321 tattctcgat attcgcccgc tgaaagaaga aaccggcatc agcgagctgg atattgccaa
     4381 gcgcctgatc gactacggtt tccacgcgcc gacgatgtcg ttcccggtgg cgggtacgct
     4441 gatggttgaa ccgactgaat ctgaaagcaa agtggaactg gatcgcttta tcgacgcgat
     4501 gctggctatc cgcgcagaaa ttgaccaggt gaaagccggt gtctggccgc tggaagataa
     4561 cccgctggtg aacgcgccgc acattcagag cgaactggtc gccgagtggg cgcatccgta
     4621 cagccgtgaa gttgcggtat tcccggcagg tgtggcagac aaatactggc cgacagtgaa
     4681 acgtctggat gatgtttacg gcgaccgtaa cctgttctgc tcctgcgtac cgattagcga
     4741 ataccagtaa ttcactgatt cgactatttt ctaaaggcgc ttcggcgcct ttttagcttg
     4801 ttaataaagt gcgctttgtg tgtttcggct gaggcgtaat ataaaatatt gcgagctcaa
     4861 tatattaaaa gagtgccagg cctgatgagc gtagcgcatc aggcacatcg gctaattaca
     4921 gattctcgcc gttgctggca atcacctctt tgtaccagtt aaagctcttc ttacgtgaac
     4981 gcgacatatc gccagtaccg tcgtcatgtt tattcacata gataaagccg tagcgtttgc
     5041 tgtactgccc ggtggtgaac gacacgcagt cgatgcagcc ccacggtgtg tagcccatca
     5101 gatccacgcc atcgtaagtc accgcttttt tcatctcttc gatatgggcg cgcaggtagt
     5161 caatgcggta gtcgtcgttg atgctgccat cttcttccac tttgtcgtaa gcgccaaaac
     5221 cgttttcgac aataaacagc ggcctctgat aacgctcata cagttcgcaa agggcatagc
     5281 gcagacctac tggatcaatc tgccagcccc agtcagacgc tttaacatac gggttcggta
     5341 cgctgccttc gaaaccagag atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg
     5401 tcatgtaata gctgaaacca agataatcgc aggtgccttt gcgcagtaca tccagatcgc
     5461 catcttccat tttgatgtta aatccgcgac gctcccactc gttcaacaca taggacgggt
     5521 aatagccgcg tagctgcaca tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga
     5581 acatcacatc ttccgggtta caggagtagg gatacagcgg caccatcgcc agcatacagc
     5641 cgactttcat ctccgggtta atgcgacgcg cagctttcac cgccagggcg ctggcgacaa
     5701 actggtgatg cagcacctga tacatcgtct cttccgggtt ctcatgctcg gtataaacca
     5761 cgccggagca gcagtaaccg aacagcggtg cacgccagtt acgctggttg ttaatttcgt
     5821 tgaaggtcat ccagtatttg actttatgct tatagcgttc aaacaccact tccgcgaaac
     5881 ggacaaagaa atcaaccact ttacggttgg tccagctacc gtattgctgc accagatgca
     5941 gcggcatttc aaagtgggag agggtgatca ccggttcgat gttgtatttc agcagttcat
     6001 cgaacatatc atcgtagaac ttcagccctt cttcgtttgg ctgagcttca tcgccttttg
     6061 gaaaaatgcg cgtccaggcg atggaagtcc gaaaacattt gaagcccatt tcggcaaata
     6121 gcttgatgtc ttccttgtag tgaccataaa aatcaacggc ttcatggttt ggatagtatt
     6181 tgcctggcaa gacttctttg gttatttcgc gcggcacgcc gtgtgcgcca ccggtcagta
     6241 cgtcacaaat actcggccct ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg
     6301 caactgcgcc gccccataag aaatctttcg gtaaggtgag ttttttcact atcataggct
     6361 ccagatgagt caattcttac aggaaatagt accatgcagc caaacgacat cacttttttt
     6421 caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg cgacgagact
     6481 gaatcgcact tcaaaacggg tgatgtgctt cgtgtcgggc gttttgaaga tgacggttat
     6541 ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacgct gacggaaaaa
     6601 catgcagagc aggaaaatat gaccctgact gaactgaaaa aggttattgc tgatatctat
     6661 cccggtcaga cacagtttta tgtgattgaa tttaaatgtc tttaaattga actcacgcct
     6721 gttagttgaa atttcgcatt taatttcatg atttatcgga gattaatccc aaatcagaat
     6781 ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag aggtatacat
     6841 gtgcgtgtac gtttctccgg agtcagttat ggttcagaag cccctcatta agcagggata
     6901 ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggctgg tgtttggtat
     6961 cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagtg ctacagcgat
     7021 aaccagttac agcctttatg gcggcagtat gatcctgctg ttcctcgctt cgacgctcta
     7081 tcacgccatt ccccatcaac gggcaaaaat gtggctgaag aaatttgacc attgcgctat
     7141 ttacctgttg attgccggaa cctacacgcc gtttttgctg gtggggctgg attctccgtt
     7201 agcgcgcggg ttgatgattg ttatctggag cctggcattg ctggggattc tgttcaaact
     7261 gaccatcgcg caccgattca aaattttatc tctggtgact tatctggcga tgggctggct
     7321 gtcgctggtg gtaatttatg aaatggcagt taagctcgcg gcgggcagcg ttaccttact
     7381 ggcggtaggc ggcgtggttt attcgctcgg ggtgattttc tacgtctgca aacgcattcc
     7441 atacaaccat gccatctggc acggcttcgt gctcggcggt agtgtgtgcc actttctggc
     7501 gatctatttg tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg ataagacgcg
     7561 ccagcgtcgc atcaggcatc ggagcgcaac cgccggatgc ggcgtgaacg ccttatccga
     7621 tctacgggcc tcgtccctgt tgtgataaag gcatcaaatc ccagacatga taagacgcgt
     7681 cagcgtcgca tcaggcgata aatgcctgat gcgatacaga gatttactct tcgagcgaat
     7741 acggcagcgg ctcgatatgc agtgtattcg catcgtcgcg tacgcggaag atgctgtccg
     7801 gttccatatc gttattcatg acgacctgta ccacgacctg accatcttcc agttttaccg
     7861 cagccagcac cgtaccggta cgacgccagt tctcgcccat tttcagctct aagtcttcac
     7921 cagcttccgg cagtcggctg gcgctacctg ccagcaacca gagcgcacgt ttattggcac
     7981 cacggaattt tgctcgcgcc accatctctt gtccggtata acagcctttc ttaaagctga
     8041 taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg ttcgcggcat
     8101 caatcaccgg gaaacccgct tcaatgttta atgccagcca ctgttggcta ttgttcagtt
     8161 ccgcttcacc gcgcagttta tcggtcagca tattggcagt agcttcatcg gttacgatca
     8221 ggaaacgttc tgccgggtgt tcaaaccata gcaaagtggt cgcgccttct ttaactacct
     8281 gtttttctct cgaaggtagt tcactaaaga tattggccag cgcggcgcgc gcctgaaaac
     8341 cggcaacacc aagcagcaca cgctcgtcgt ctggcgcgat ggtcacttta gagaataccg
     8401 catatttttt cagttcagtc agctgcggtt cacgcacact gcgccgttca atccacgcaa
     8461 agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttacctttg gcgtcgcaat
     8521 gggcggcgag cagatgctga ttttctgtca tctggctgac atctgctgtc acctgaccct
     8581 gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca
     8641 tcagcgtcag cggtaaacgg gcagaagccg taggctgacg gggaggaaaa ggtgtaaaag
     8701 ccataataga gtcctgagtt gcttaacggc gtattaataa ctaatggtaa aagagctatg
     8761 aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac ttccaaaaaa
     8821 atgaagccct tgaattgttg tgaattaatt tcgcgatcgc gattgtgttt actgatattc
     8881 ccgtaaaaga agtcgaaaaa cacgttacaa tagcggggta ctggattttc gtgagacagg
     8941 aagacgatgg acattaacaa caaagcccgc attcattggg catgccgccg tggtatgcgc
     9001 gaactcgata tttcaatcat gccgtttttc gaacatgagt acgacagctt aagcgatgac
     9061 gaaaaacgca tctttattcg tctgctggaa tgtgacgatc cagacctgtt taactggctg
     9121 atgaatcacg gtaaaccggc cgatgcagaa ctggaaatga tggtccgact catccagaca
     9181 cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg
     9241 gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc cctggccgct
     9301 cagttacacc ccgttatgga tggtgttact ttcgctggtg gtgtttgatt gcgttcgcag
     9361 ccagcggcgt attaatgctc gccaggggga aattcgcttg ttgatggacg ggcgtttgcg
     9421 ttggcaaggg caggagtgga gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat
     9481 gctgcgttta cgttctgatg gcggtaaacg gcaacattta tggctggcag ccgacagcat
     9541 ggacgaagcc gaatggcggg atttacggcg gatattgttg caacaagaga cgcaaagata
     9601 aagatgacgg caaagcatat gtgctaagcc gtcagtgaca tcaggcgtaa tgctctgcca
     9661 tttcgttgag gatttgctcg caccagctct gaatacgctc gtcgctaagg tcatactggt
     9721 tagtttcatc cagcgccaga cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc
     9781 tggtaaattc atatccttcc gttggccagt agccgacgaa ctttacgcct ttggtcgaga
     9841 gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc agttgatcgc
     9901 caagcccata cagcgcaaca attttacctt cgaggttcag gtcgtcgagc tgatcccaga
     9961 cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc agaatgagca
    10021 catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag gtcaccagtt
    10081 ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag gtgctggaac
    10141 cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc ttttgtggct
    10201 agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa atgagtgacc
    10261 ataaggggcg caagtgaaac aggatctggc acgcatcgag cagtttcttg atgctctgtg
    10321 gctggaaaaa aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat
    10381 ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg acgatttgca
    10441 ggcattactg gcagaacggc tggagggcgg gtataaagcc accagctcag cgcgtttgct
    10501 gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc
    10561 cagtgcgcat ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt taagcgaagc
    10621 gcaggttgaa cgtttattac aggcaccatt aattgatcag ccactggagc tacgcgataa
    10681 agccatgctt gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg tcgggctgac
    10741 aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga
    10801 gcgcctggtg ccgttaggtg aagaggcggt ttactggctg gaaacctatc tggaacatgg
    10861 gcgtccgtgg ctactgaatg gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca
    10921 gcagatgacg cgacagacct tctggcaccg cattaaacat tatgccgtgc tggcgggtat
    10981 cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc acttattaaa
    11041 tcatggtgcg gatttacgcg tggtgcagat gctactgggc cacagcgatc tctccaccac
    11101 gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc
    11161 gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag aaaggtttta tgttgtttac
    11221 tttgttagcg gcgttttcag gctttgctca ggctgatgac gcggcaattc aacaaacgtt
    11281 agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg gcatgaagac
    11341 agttctgact aacagcggtg tgttgtacat caccgatgat ggtaaacata tcattcaggg
    11401 gccaatgtat gacgttagcg gcacggctcc ggtcaatgtc accaataaga tgttgctaaa
    11461 gcagttgaat gcgcttgaaa aagagatgat cgtttataaa gcgccgcagg aaaaacacgt
    11521 catcaccgtg tttactgata ttacctgtgg ttactgccac aaactgcatg agcaaatggc
    11581 agactacaac gcactgggga tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga
    11641 cagcgatgca gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa acaaagcgtt
    11701 tgatgatgtg atggcaggta aaagcgtcgc accagccagc tgcgacgtgg atattgccga
    11761 ccattacgca cttggcgtcc agcttggcgt tagcggtact ccggcagttg tgctgagcaa
    11821 tggcacactt gttccgggtt accagccgcc gaaagagatg aaagaattcc tcgacgaaca
    11881 ccaaaaaatg accagcggta aataattcgc gtgaaacaac agatacaact tcgtcgccgt
    11941 gaagtcgatg aaacggcaga cttgcccgcg gaattgcctc ccttgctgcg ccgtttatac
    12001 gccagccggg gagtacgcag tgcgcaagaa ctggaacgca gtgttaaagg tatgctgccc
    12061 tggcagcaac tgagcggcgt cgaaaaggcc gttgagatcc tttacaacgc ttttcgcgaa
    12121 ggaacgcgga ttattgtggt cggtgatttc gacgccgacg gtgccaccag cacggctcta
    12181 agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg actacctggt accaaaccgt
    12241 ttcgaagacg gttacggctt aagcccggaa gtggtcgatc aggcccatgc ccgtggcgcg
    12301 cagttaattg tcacggtgga taacggtatt tcctcccatg cgggagttga gcacgctcgc
    12361 tcgttgggca tcccggttat tgttaccgat caccatttgc cgggcgacac attacccgca
    12421 gcggaagcga tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa atcactggca
    12481 ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgca cctttttgcg cgatcagggc
    12541 tggtttgatg agcgtaacat cgcaattcct aacctggcag aactgctgga tctggtcgcg
    12601 ctggggacag tggcggacgt cgtgccgctg gacgctaata atcgcattct gacctggcag
    12661 gggatgagtc gcatccgagc cggaaagtgc cgtccgggga ttaaagcgct gcttgaagtg
    12721 gcaaaccgtg atgcacaaaa actcgccgcc agcgatttag gttttgcgct ggggccacgt
    12781 ctcaatgctg ccggacgact ggacgatatg tccgtcggtg tggcgctgtt gttgtgcgac
    12841 aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa
    12901 gagatcgaac aaggaatgca aattgaagcc ctgaccctgt gcgagaaact ggagcgcagc
    12961 cgtgacacgc tacccggcgg gctggcaatg tatcaccccg aatggcatca gggcgttgtc
    13021 ggtattctgg cttcgcgcat caaagagcgt tttcaccgtc cggttatcgc gtttgcgcca
    13081 gcaggtgacg gtacgctgaa aggttccggt cgctccattc aggggctgca tatgcgtgat
    13141 gcgctggagc gattagacac actctaccct ggcatgatgc tgaagtttgg cggtcatgcg
    13201 atggcggcgg gtttgtcgct ggaagaggat aaattcaaac tctttcaaca acggtttggc
    13261 gaactggtta ctgagtggct ggacccttcg ctattgcaag gcgaagtggt atcagacggt
    13321 ccgttaagcc cggccgaaat gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg
    13381 tgggggcaga tgttcccgga gccgctgttt gacggtcatt tccgtctgct gcaacagcgg
    13441 ctggtgggcg aacgtcattt gaaggtgatg gtcgaaccgg tcggcggcgg tccactgctg
    13501 gatggtattg cttttaatgt cgataccgcc ctctggccgg ataacggcgt gcgcgaagtg
    13561 caactggctt ataagctcga tatcaacgag tttcgcggca accgcagcct gcaaattatc
    13621 atcgacagta tctggccaat ttagcgtcat cttctctata aaaaagagcg tggattgggt
    13681 acaatcccgc tcttatcacc gcattttgac tagctcaata aaagaaatca gaccatgttt
    13741 gaaattaatc cggtaaataa tcgcattcag gacctcacgg aacgctccga cgttcttagg
    13801 gggtatcttt gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga
    13861 acagccggat gtctggaacg aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc
    13921 cctcgaagcc gttgtcgaca ccctcgacca aatgaaacag gggctggaag atgtttctgg
    13981 tctgctggaa ctggctgtag aagctgacga cgaagaaacc tttaacgaag ccgttgctga
    14041 actcgacgcc ctggaagaaa aactggcgca gcttgagttc cgccgtatgt tctctggcga
    14101 atatgacagc gccgactgct acctcgatat tcaggcgggg tctggcggta cggaagcaca
    14161 ggactgggcg agcatgcttg agcgtatgta tctgcgctgg gcagaatcgc gtggtttcaa
    14221 aactgaaatc atcgaagagt cggaaggtga agtggcgggt attaaatccg tgacgatcaa
    14281 aatctccggc gattacgctt acggctggct gcgtacagaa actggcgttc accgcctggt
    14341 gcgtaagagc ccgtttgact ccggcggtcg tcgccatacg tcgttcagct ctgcgtttgt
    14401 ttacccggaa gttgatgatg atattgatat cgaaatcaac ccggcggatt tgcgcattga
    14461 cgtttatcgc gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg
    14521 tattacccac atcccgaccg ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa
    14581 gaacaaagac caggccatga agcagatgaa agcgaagctt tatgaactgg agatgcagaa
    14641 gaaaaatgct gagaaacagg cgatggaaga taacaaatct gacatcggct ggggcagcca
    14701 gattcgttct tatgtccttg atgactcccg cattaaagat ctgcgcaccg gggtagaaac
    14761 ccgcaacacg caggccgtgc tggacggcag cctggatcaa tttatcgaag caagtttgaa
    14821 agcagggtta tgaggaacca acatgtctga acaacacgca cagggcgctg acgcggtagt
    14881 cgatcttaac aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc gtgagcaggg
    14941 aattgccttc ccgaacgatt tccgtcgcga tcatacctct gaccaattgc acgcagaatt
    15001 cgacggtaaa gagaacgaag aactggaagc gctgaacatc gaagtcgccg ttgctggccg
    15061 catgatgacc cgccgtatta tgggtaaagc ctcttttgtt accctgcagg acgttggcgg
    15121 tcgtattcag ctgtacgttg cccgtgacga tcttccggaa ggcgtttaca acgagcagtt
    15181 caaaaaatgg gatctcggcg acatcctcgg cgcgaaaggt aaactgttca aaaccaaaac
    15241 cggcgaactg tctatccact gcaccgagct gcgtctgctg accaaagcac tgcgtccgct
    15301 gccggacaaa ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc gttatctgga
    15361 tctcatctct aacgatgaat cccgcaacac ctttaaagtg cgttcgcaga tcctctctgg
    15421 cattcgccag ttcatggtaa atcgcggctt tatggaagtt gaaacgccga tgatgcaggt
    15481 gatcccaggc ggtgcggctg cgcgtccgtt tatcacccat cataacgcgc tggatcttga
    15541 catgtacctg cgtatcgcgc cggaactgta cctcaagcgt ctggtggtcg gtggcttcga
    15601 gcgtgtattc gaaatcaacc gtaacttccg taacgaaggt atttccgtac gtcataaccc
    15661 agagttcacc atgatggaac tctatatggc ttacgcagat tacaaagatc tgatcgagct
    15721 gaccgaatcg ctgttccgta ctctggcaca ggatattctc ggtaagacgg aagtgaccta
    15781 cggcgacgtg acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat
    15841 caagaaatat cgcccggaaa ccgacatggc ggatctggac aacttcgact ctgcaaaagc
    15901 gattgctgaa tctatcggca tccacgttga gaagagctgg ggtctgggcc gtatcgttac
    15961 cgagatcttc gaagaagtgg cagaagcaca tctgattcag ccgaccttca ttactgaata
    16021 tccggcagaa gtttctccgc tggcgcgtcg taacgacgtt aacccggaaa tcacagaccg
    16081 ctttgagttc ttcattggtg gtcgtgaaat cggtaacggc tttagcgagc tgaatgacgc
    16141 ggaagatcag gcgcagcgct tcctggatca ggttgccgcg aaagacgcag gtgacgacga
    16201 agcgatgttc tacgacgaag attacgtcac cgcactggaa cacggcttac cgccgacagc
    16261 aggtctggga attggtatcg accgtatggt aatgctgttc accaacagcc ataccatccg
    16321 cgacgttatt ctgttcccgg cgatgcgtcc agtaaaataa gcattacgtt atgctcataa
    16381 ccccggcaaa tgtcggggtt ttttaattgt gctgcgcgaa agcagacaac aattttcttg
    16441 cttcactatt ggctgcctgc attaccatcc acggactgaa cgcccacggc gtggcatcaa
    16501 taccgtgtaa tacatctgct aaatcacacc attgataatc catcacttca tcatcgttga
    16561 tctgtaacgc actgttggtg cgtgcggcaa ataccggaca cacttcattt tccacaatgc
    16621 cattcggatc ggtggcgcga tagcgaaagt caggatagat agattcagga ggcgtaattt
    16681 ccacgccaag ctcataacgg caacggcgga tcactgcgtc ttcgttgctt tctcccagtt
    16741 gtgggtgccc acaaaccgag ttagtccaca cgccaggcca tgcttttttg ctcagtgcgc
    16801 ggcgggtaac taataattgc cccttggcat taaacagcca actggagaac gcgagatgta
    16861 agagggtgtc tgccgtgtgt gcggcatact tttccagcgt acccgtggga actccctgtg
    16921 cattcaataa aatgacgtgt tccgtttgca taatttctca catgtaattc tgatcactca
    16981 tcattataga taattgaaga gtgaaatgat taaccaagtg agcgtttatc gacaaccgcc
    17041 cgttttgagc ggatgccgac aggtaaaaac catttaaaaa agtagcatca tatatttgaa
    17101 gcgactttca atggccattg caacacagtc agaaatgaca ggaatagaga gagtaaaaag
    17161 caacagcaga ctgaatatag cgcgcattaa aagattcata ttattctcca tgctcatttt
    17221 tcttcagctt gcgcatcaga agccacatac gtactgtcct gacttttaaa tctttatcag
    17281 agacggattc cttcaccagg tcagcatgat gctgatagcc attaaagaag acgttcagca
    17341 caacggcgct aagggttgcg agcataatgc cgctatgtag cagcggttgc agtacggccg
    17401 gtaacttaga aaagaaatcg tgagagagcg tcggagtcat gccaacgccg agactgatcg
    17461 ccacaatata gaggttgtaa cggttggtgg tgtagttaca gcgcgacaga attcgaatcc
    17521 ctgtcgccag taccatgccg aacatcacca gcccagcgcc gcccagcaca aattgcggaa
    17581 tggaggcgac cagcaccgcc atttttggca ccatgccaaa caggattaat ataattcccg
    17641 aagaaataca cacccagcgg ctatgaacac gcgtcacgct aaccaggcca acgttttgtg
    17701 aaaatgacgt gtgggggaag ctgttaaacg ttccgcctat cattgtccct acgccatcga
    17761 cacgcagccc gcgaataata tcgtgtgaag agagtttacg accgactatt tcacccagtg
    17821 ccaggaacat ccccatcgac tcgataaaca cgatgattaa cacggcagtc atggtcagaa
    17881 tggaaacggg atcgaaaatc ggcataccaa acgacatcgg ggtgacaatc gcaaaccacg
    17941 aggcatcatg tagcccggat aagttgactt cattcatcat ccacgaaagt aaaaagccaa
    18001 atacaatccc cagtaatacg gcgacgttgg acataaatcc tttcgcatag cgagtaatga
    18061 gcaagataaa aattaagacg gcaaaggaga tacctaaata aacgggatta ccatattgcg
    18121 gattcccttt acctccggcg gcccagtcaa tacccacctg aatgatgcta agcccaatag
    18181 aagtaataac cacaccggta accagtggcg ggaataaagg catcaagcga ccgataagtg
    18241 gcgctaataa tgtggtgata aaacccgcgg cgatagtggc accaaagatc cccagcaggc
    18301 cgatatccgg gttcatccca atggctatca ttggtgttac agcagcaaag gtcaccgaca
    18361 taatcaccgg caggcggatc cccataaagc ggccgatacc gatacattgc aataatgtga
    18421 cgatcccgca gcaaaagaga tccgagctaa tgagcatcgc aatagcttct tttgagaggc
    18481 ccagtcggtc accaatcata agaggaacag cgactgcacc tgcgtacatg acaaggacgt
    18541 gttgcagacc gagtatgatc agctttcctg gtgataatat gcggtcaacc tcatcagttg
    18601 ggcggtcttg cccagaagat gagggaagtt gggaatctat ggcgctcatc agtgtttctc
    18661 ctttcgcccg ccagagtaaa aagcaaccgt ttacgttaat gaaactgtct aaacacatgc
    18721 tcgtggagtg ccagtttttg cgaggaaggg gagttagaga caggaattgc ggaggcaatc
    18781 acataatcgt ggttaaacca cgaaaatgag aagagaacct gacaatggaa ttatctgtaa
    18841 ttttctcata ttgatatttc attaatcatt ctggcatgtc gtactctcgc atttaatcgt
    18901 ttttatctgg atagcactct tttgatcggc aatgttgaaa taatgaaagc ggcatagatt
    18961 gaggggcaat aatttgaact cagtcgcaaa attataatta aaacggtcat gctacctaat
    19021 atatgcccgt ttattgcatc gcgaaatttg agaagacata actcattaaa tgaaagggat
    19081 gcaaatgaat aagtttatcg ctgctgaagc tgcggaatgt ataggctgtc atgcttgtga
    19141 aattgcctgt gcggtggcac acaatcaaga aaactggccg ctgagtcaca gtgactttag
    19201 accgcgtatc cacgttgtag ggaaaggcca ggctgcgaat ccggtggcct gccatcactg
    19261 caacaatgcc ccttgcgtta cggcttgtcc ggttaatgct ctgactttcc agtccgatag
    19321 cgtacaactg gacgagcaaa aatgtattgg ttgtaaaaga tgcgcaatcg cttgcccctt
    19381 tggcgtcgtt gagatggtcg atacgattgc gcagaaatgc gacctttgta accagcgcag
    19441 ttccggcacg caagcctgta ttgaagtctg cccaacacag gcgttacgac tgatggacga
    19501 taaagggtta cagcagataa aggtggcccg ccagcgcaaa acggcagcag gaaaagcgtc
    19561 atcagacgct cagccatctc gcagtgcagc gttgctcccc gttaactcgc gtaaaggcgc
    19621 agataaaatt tcagcgagtg aacggaaaac ccactttggc gaaatatatt gcgggctgga
    19681 tccacaacaa gcgacttatg agagtgaccg ctgtgtttat tgtgccgaaa aagctaactg
    19741 caactggcat tgcccgctgc ataacgctat tccggattac atccgtctgg tacaggaagg
    19801 aaagattatt gaagcggcag aactttgcca ccagaccagt tccttaccag aaatctgcgg
    19861 cagggtatgt ccacaggacc gtctttgtga aggcgcatgt actttgaaag atcactctgg
    19921 cgcagtcact atcggtaatc tggaacgcta catcaccgat accgcgctgg cgatgggctg
    19981 gtgtcccgat gtcagcaaag ttgttccccg tagcgaaaaa gtggcggtga ttggcgctgg
    20041 gccagcaggg ttagggtgtg ctgatattct ggcgcgcgca ggagttcagg ttgatgtctt
    20101 tgatcgccat ccagaaattg gcggtatgct gacttttggc atacctcctt tcaaactcga
    20161 taaaacggta ttaagccagc ggcgagagat attcaccgca atgggaatcg actttcatct
    20221 taactgtgaa attggccgcg atattacctt tagcgattta acttctgaat atgatgcagt
    20281 tttcatcggc gtggggactt acgggatgat gcgagctgat ctgccgcatg aagatgcgcc
    20341 cggtgtcatt caggctctac cgttcttgac tgcccatacc cgccagctca tggggttgcc
    20401 ggagtctgaa gagtatccgc tgacggacgt ggaaggtaag cgagtcgtgg tattgggcgg
    20461 tggcgatacg acaatggatt gtttgcggac ttccatccgc ctcaatgccg ccagcgtgac
    20521 ctgcgcgtat cgtcgtgatg aagtcagtat gccgggctcg cgcaaagagg tggtcaatgc
    20581 gcgcgaggaa ggtgtcgagt ttcaattcaa tgttcagccg caatatatcg cttgtgacga
    20641 agatgggcgt ttaactgcgg tgggcctgat tcgtaccgcc atgggtgagc cggggccgga
    20701 tggtcgccgt cgtcctcgtc cggttgcggg ttcagagttt gaattgcccg cagatgttct
    20761 cattatggcc tttggtttcc aggcgcatgc catgccgtgg ttgcagggca gcggaattaa
    20821 actcgataaa tggggcctga ttcaaaccgg cgacgttggg tatttaccta cccagacgca
    20881 tctgaaaaaa gtctttgctg gtggggatgc agttcatggc gcggatctgg ttgtcactgc
    20941 catggccgca ggaaggcagg cggcgcgcga tatgttaact ctgtttgata cgaaggcatc
    21001 gtgatgaaat cgttaattat cgttaatccg gctgactgta ttggctgccg tacttgtgaa
    21061 gtggcctgtg tggtcgctca cccttcagaa caggagttga atgccgatgt ctttttaccc
    21121 cggctgaagg tacagcgact ggacagcatc agtgcgccgg tgatgtgcca tcagtgtgaa
    21181 aacgcccctt gtgttggcgc ttgccccgtg ggggcgctga cgatgggcga gcaggtggtg
    21241 caggccaatt ctgcccgttg tattggctgt cagagctgcg tcagtgcgtg cccgtttggg
    21301 atgatcacca ttcagtcatt gccgggggat acccggcaac aaatcgtgaa atgcgatctc
    21361 tgtgaacagc gggaagaggg gccagcctgc gttgaatctt gcccaacaca ggcgctgcag
    21421 ttgcttactg aaagagaact caggcgagtc cgccagcagc gtattgttgc cagcggtgag
    21481 aatcctctct gaaagaaatc tccgcgctga aaggcgcgga gaatctatta gattgcccaa
    21541 ccacccgcgt aaaatgcgac cagtgcggca gtaatgatca ccgtaccaat attcagcttt
    21601 tgccattcgc gtgcaaagac gcggcctacg accagtgtca caaagcccag cataataccg
    21661 gtaacgatat tacaagtcag aacgatgaac acggcacaca ccaggccagc catagcgtca
    21721 ataaaatcat tgaaatccag cttcgagacg ttacttaaca tcagcaaacc tacgtacatc
    21781 agagcgggtg cagtggcgta accagggatc aaaaatgaca gcggtgataa gaacagaatc
    21841 agcaggaata acgcccccac tacggttgcg gttaaacctg ttttaccccc ggcggcggtt
    21901 cctgccgctg attcgatata aaccgctgcg ggcgctgcgc ccaccaggcc ggagaatatt
    21961 gaacttactg agtcactggt cagggctttg ccgccgttga tgatctggtt gtctttatcc
    22021 aacaaattcg cctgaccggc gacggcacgg atggtgccag tagcgtcgaa cactgcggtc
    22081 atcaccaatg ccagtacact cggaagtaca gttggctgga gtgcacccat aatatcgagg
    22141 ctgaaaatca gagacttacc atcttcgcca gtcaggcttg gcatcgccac cagaccgtgg
    22201 tatttcaccg ctgggtcaaa gattaagccg atgatcgaaa ttgcaataat caccaacaag
    22261 atcccgccgg gtacgcgaca cttctccagg ccgaagatga ccgccagccc cagcaagctc
    22321 atcatcaccg ggaaggaggt aaacgcaccg agcgcaactg gcaagccttc aatcgggttt
    22381 ttgataacca tacccacacc gttagcagca atcagcagca gaaacagccc gataccgata
    22441 cctgtaccgt gagcgatacc catcggcaaa ttacgtaaga tccaggtacg cacaccggtt
    22501 acggaaatgg cggtgaagat gacgcccatc agaaataccg cgcccagtgc gacaggaacg
    22561 ctaatttgtt gcccgagtac cagactgaat gcggtaaacg ccgtcaagga aatcgcgcaa
    22621 ccaatcgcca ttggcaaatt agcccataat cccatcagca acgagccgaa gcccgcgacc
    22681 agacaggtgg caacaaacac agctgcggga ggaaaacctg ctttgcccag cattcccgga
    22741 acgacgataa cggaataaac catggccaga aaggtcgtta agccagccag tacttcctga
    22801 cgaacggtac tgccacgagc ggtaatttta aaataattat ccagcgcgcc ctgtggcttt
    22861 ggtgcgtccg gtgtttgtag gatgtctcca gacataatga tgtcctcacg aggatgttaa
    22921 agttttatta ttagttgcgt tcgtacacca gacgaccatc aacgtaggtg cggtagatcg
    22981 aacggtcatc gcccaacgtc atcatcacga acaatttgtc gactaaagaa acagagttgt
    23041 catagcgcag ctgctgtagc ggagtggcgg tgggttccat caccacgaaa tcagcctctt
    23101 tgccaggtaa aaagttgcca atcaaatcgt caaggcccag agatttcgct ccgccgagcg
    23161 tggccaggta aaacgcttca tatgccgaga ggcgatagcc ttgtaattgc aatactttgt
    23221 aggcttcgtt cagcgtttgc agcatgttga aagtggttcc ggcaccgata tccgttccca
    23281 tgcccacttt aactttcttc tgccatgctt ttttcaagtt gaataagccg ctgccgaggt
    23341 aaaggttgga ggtcggacag aaagcaatgc tggatttggt ttcgctgaga cgatcccact
    23401 ctttttcttc gagatggacg cagtgagcaa agacacagtt tttaccggtc aggccgtact
    23461 gatggtaaac atccagataa ccatcatggt caggataaag cgatttcacc caggcaattt
    23521 catctttgtt ttcacagaga tgggtatgta cccacgtatc cggatattct tctttcaggc
    23581 gttgcgccat cgccatctgt tcaggagatg aggtcggggc gaagcgtggc gtaatcgcat
    23641 atagcagacg accatttttg tgccagcgtt caatcagttc tttgctttgg tgatagctgc
    23701 tttcggcagt gtcgagcaga taatccggtg cgttacggtc catcatcacc ttaccggcaa
    23761 tcatacgcat attgatatga ctggcggctt caaacagcgc atcaacggat tgcggatgaa
    23821 cagtgccaaa caccagcgcc gtggtggttc cgttacgtaa aagctgcttg atgaagaacg
    23881 ccgacatttc gcgggcgtac tctaaatcct cataacgccg ttcagtaggg aaggtgtgtt
    23941 tattcaacca ctccagcaat tgctcaccat aggcccccac catttcactt tgcggataat
    24001 ggatatgtgt atcgacaaag cccggtacta tcagtttgcc gcgatagtcg cgcacgcgaa
    24061 tggtgtcagg aatttgatgc tttccgtttt cccattcgcc aaaccattcc acttttccct
    24121 gtttaatgag taataaacca tcctcaataa accgcagcgc agaggcaatc tcttccgggt
    24181 tatcgaccgt acgggtgaca tcaataaaac tgcctcgtac cgcttttaac gtgtgttctc
    24241 ctgacatcat tgactccttt actgttaatc catctcttcc gctgaggtaa taccaggcag
    24301 aacgttttcc tgtcggtagc caccagggag gataatattt aataaaatcg cagttaaccc
    24361 gccagcacaa atagggtttt caactaatac ataaatagag gctggcaata ttttaaaaat
    24421 ttcgggatcg taggagacgc caagccctaa acctaaagaa gtggcgacaa taagtgtttc
    24481 acgccgcttt aatccgttgg tgatgatgat gcgaatccca gcgatggcaa tcatggaaaa
    24541 catcaacgtc attgcgcctc ccagaactgc cgagggaatg gtcgtgaaga agccgccaat
    24601 catcggaaat aagccgagga taaccagcat taccgcgatg gttcgcccga cataacgtga
    24661 agcgacgcca gtcatctgaa taaccccatt attttgcgca aacgtggtta atggtaatga
    24721 accgacagcg gaggcgataa cagaaaccag accatctgcc agcacgccgc ctttcagtcg
    24781 ggactgatac tcttccccct gaatggggcg gcgggaaacc attgccgtgg cggtgatatc
    24841 gccgactgct tccagcacgc taagcagata aatcgtgcca accaccagga actgatggaa
    24901 gctaaaacta aagccgtatt tgaacggatg cgggatggtg attaacggca aattgcgcat
    24961 actgctgaaa tccaccatgc ccaggcataa cgatgcgata tagccgacac atagcccaat
    25021 ggcgatccct cccatgcgta gcaacggact gcgacagcag ttaaagccga tcaccacaat
    25081 taaaaccaat aaaccaacgc cgagatgttc gtaattgccg aacgtaccgc tgcttttggc
    25141 tgcaaatcct ccaccaaaat cgataatgcc gactttaatc aggcttaagc cgatcatcag
    25201 taccacaata ccgctgacgg taggcgtaat aacccgacgt aaatagggca agataaatga
    25261 agatccgaca accagaaatg cgccaacgaa ggagacgccg agaagcgacg acatgattaa
    25321 ctcttcgtga aaaccgtcgc ttttcatgct gctgcccagc gcaatcatga ccgtaacaaa
    25381 tgaaaaattg actgactgaa ttgagagtag gccagaaccg acgatgccgt agcggtttac
    25441 ttgtaaccag gtaccaatac cagaggcgat catcgccata gaaacaagat aggcagttgt
    25501 ttcagcggaa agctgtaagg ccgcacccac gattaacgcg ggggttacca tcggaacgaa
    25561 aattgccaac agatgggtaa tggcaccaac gagagcctga tgaaagggag ggcgatcctc
    25621 cagttcaaat ataaggtcag aacctgcatg gtttatatca gacatccctt cactcctttt
    25681 aaagtaaatt tgatcatctg aattagtaaa ataaattccc acgaatttgg ggcgcaggac
    25741 gccttgagaa cggcttatct cattttcgca gtcactattt agtcatcgtt caaacagaga
    25801 aacaggccgc cgaagcggcc atttattatt tatatttttt ccagcgcagt gagtattttc
    25861 tccggtgtga aatgccattc gcgtaaccag atgccgcatg catcgtgaat tgcggtagca
    25921 atcgccggag ctgcgccatt tacaccgatt tccgagatcg atttcgcccc gaacgggccg
    25981 actttatcgt cgctcggcac cagcacagcg cggaaatcac gcggaatgtc accaattttc
    26041 ggtgcgccgt aactgcgtaa atcacgcgtt aacgggtgac cttcggcgtc ataaatgatc
    26101 tcttcgctca tactgtggcc gatagcacgc agggtggcac cgtagatttg tcccaacgct
    26161 aactctggat tgaccggtgt accgcagtcc agcaaggcgt agaatttatc caggcggatt
    26221 tcacccgtac gcgtgttgac ggcaacttca gcgaagtttg cgccatacgg gaaggcgaaa
    26281 tcaggcgtga tataactgcc agttcccacc agtgaaccaa agccggtgcc ggtttcgcct
    26341 ttatgggcaa tatccccgaa actaacttcg cctttcttgc cgcgcacgac gcccggcgtt
    26401 gccagttgaa catctgccac tggctcaccc aacatttgcg cgccgtggaa cagaattttc
    26461 tcccgtagat tttccgctgc caaacgcgcc gcgttacccg agaagcaagt accggacgag
    26521 gcatatgcgc ctttatcaaa caacgcatga tcggtatcac cggagataac atgcacgtcc
    26581 tgcggtgggc agtgcagcac ttctgctgcc agtttcgtca ctacagtatc cagaccagta
    26641 ccaatatccg caccgccaga atgaacgata aaggtgccgt ccgattccag tttgatcatg
    26701 cagttagcct gatcgatatc cgggataccc gatttctgca tgataatggc gacgccgcga
    26761 ccgatgtgcc agtcaccatt ttgcggtttc ggtgacgacc attggatcat ctcgcgaccc
    26821 tgacgcagga tctcttccag tgcacagctg gcggcggaag gaacggaggt cggcgcttta
    26881 ccttcaccga ttgcaccgag aattttcagc tcttgccctt cgtgtacccg gttacgttcg
    26941 ataatttcca gttggtcgat ctgtaactgt tcagccagtt ccgctaatgc catggtgata
    27001 gcgaagttac ctttcggtgc gccataaccc tgataagcac catttgggca aatgttgctg
    27061 tagtaggtgg tgacctggaa atcgacgtta tcgcacggat ataacggcag cgacagcgcc
    27121 ggtccgttac acggtacggt gagtgagtgg ttgccgtaag ggccagtgtt ggcgcggaaa
    27181 tccatcttca ctgccgtcag gcgaccatct tttttcgctc ccagtttgac ggtgactttc
    27241 gcgacgtgac gagaggtgtt agcaataaac tcttcttcac gggtgtagcg gaacagtacc
    27301 ggacgcccgg tcacgcaggt tgcccaggcg cacacttctt ccagcaggat gtcctgtttg
    27361 gaaccaaaac cgccgccaac tcgctcttta atgacatgaa ctttatgctg tttcatgccc
    27421 acgaggcgcg cgacctggcg gcgtaagtgc catggtacct gggtggaggc gtggataacc
    27481 agacgatcgc cgtccatacg agtaaagcag atatgtgttt cagtcgggca ctgctgcgct
    27541 tgcgttgagt tataggttcg ctcaatgatc acatcggcat cggcaaagcc tttgtccata
    27601 tcgccaatat gaccatgaat actggcggcg atatttttgc gagggcgaga accgatcggg
    27661 aagttgatga tcatatgctc gccgcgctgg gctgcatggc tgttatcgtc ttccagagta
    27721 tctggcgcac cagcaacata caccaccggt tcatcgtgca cgacaggcgc atcttccgcc
    27781 attgcttcgt cgatcgacat taccggctta agcacttcat attcaacgtc gatgagcttc
    27841 agtgcttcga gcgcaatttc ttcactttct gcgacgaccg cagcaacacg atcgccgacg
    27901 tgacgcattt tcttgccgaa catacggcgg tcaagcggtg acggttccgg tgcgctctga
    27961 ccacccgggg tatagtagat atccgggcaa ttcaggtgag taataacgtg aacgacgccc
    28021 ggtaaggctt cagctttgct gacatccaga tgagtaatca gtgcgtgagc gtgtgggcta
    28081 cgtaacattt taatgacgca ggcgtcagcc gttacgcggt cttcaacata gcagggtttc
    28141 gcctgcacca ttttcgcggc atcagtttta ggataatgct taccaatgac ttctaggtcg
    28201 tcacggaaag tcggagcgat atcaatggtg gcctgcggat tatttttacg tgcaaccgcc
    28261 agttcaatga cctgatagta ttgctgccag ccagcatcgc ggctgaacaa accagaaagc
    28321 gcgtcgtcga tctcttcgcg ggtaggtgcg gcgatgcgat cgagaagatc ggtgataatc
    28381 agagctgcag ctggatcgtt ataaccagac tgcaccacgc caacatcaac cattgcctgt
    28441 tgaaccagac ttaactcgtt ccatttgccc agagattctg cggtacgaat atctgccttc
    28501 tctaactgtg cggcaataag caaggacgcg ttaacgatat taccgttaaa gattattgcg
    28561 tcagaaccgg caaacccgaa accatcatca ctgttgcgta cagagtgcat tcccatgtta
    28621 aacaacagct tttgcacgtt ttcgcctgga ttaacggtta gctcctgagg cgcgccattt
    28681 aaagtaaagt ggatgatcat acggcttcct ccccagcctg ttggcagtcg gcatacaggt
    28741 cggctaccag aactcccgtg atatagcgtt tataggccac gctgccccgc aaatcttcct
    28801 gcgggaaaat ggcgttggcg acagcctgtt caagtgcatt gccttccaga ttttgttttt
    28861 cgacatcatg cagacgcagt gctttactgg cgacgccatc cagcgcaatt cgcataccgt
    28921 cgtggtctgt cattgcaacg gctgccgtca cgacggttaa acctgcctga gaacggctaa
    28981 ttttgcgggt cgcacaggtg cgatacggat ctttaatgat aatttcggtt agcaggcgat
    29041 cgcatgggca ggccaggtag tcctcgattg acagcgtttc gccgttgcca aaaaccagtt
    29101 cagcatccag tgccaacagg acgggaagca gcaccgactc ttcctggcgg gcggcgattt
    29161 caccaccaat ggtcgactga ttacgaacat ggcgtgagta aacaaaaccg agggcttcac
    29221 gcagcgctgc aggaataaat cgcgcatcac gcagtggctg caagcgagac attgcgccaa
    29281 tccgcagtgc accgttatcc cagtcaaccc agtccagttc cagatcctgt aaggaaatgg
    29341 caatcttttt atcggtacgg gttggtgtag cattgagttt gctgcccccg gcgaaccaga
    29401 cggcttcatc ctggtagcgg cgcttcagtt ccagcgcctg ttcgacagag tcgggcctga
    29461 aaaattgttc aatcatcttc tttccttatt taaccctctt ccctgcggga agagggcatc
    29521 tgtcatttat gccagcgcat ccatccgacg ccacatactg gcagcggctt ttctggcttg
    29581 tgcataaatg gaatcgcaat cgaagttaaa ctgacggtct tcatagacca tcacaccatt
    29641 gaccatcacg ctgtgaacgc tgcctgagcc cataccgaaa gcgatatgcc cggcgatatt
    29701 gtctgccagc agcggcgtcg gcgagttgta atcgcaaatg gttaaatcag ctttgtaacc
    29761 ggcttccaga agcccaaatt tcgcgccaaa gttgcggctc atcagttcgt taccgttagt
    29821 cagggctttg gcaaaactgt caggccacag cggaccaccc gcatcgcgat gtttaaagaa
    29881 ggcaaatttc atctcttcaa acatgtccga accaatgccg tccgttccca acgccagatt
    29941 gcggatgtcg ctaaggtgat ggttgtagcc gacatggttg ttcatgtttg aacgggcgtt
    30001 atgcaccagg aacgcatcgc gctgattgag tagggtgatg tcatctttcg acaagtacag
    30061 cccatgagcg accagcgttt tgctgtcgat gagatcgaat tgcgccagtc gtgccagcag
    30121 gtctttgccg taccagtggt gactgtagga aacgtcgtaa aggtcttccg cagcgtgaat
    30181 atgcaaacca cggcctgtgg ctttcacggc ttcacgcagc atctccagac cggcatccgg
    30241 cacggtaaac ggcgcgtgag caccgatatg tgcttccacc agatacggct cgcttgtcgc
    30301 tttcttcgcc tcatcaatca aacgggcgaa acggatgttt tcttctacac cttcctgcaa
    30361 ctctttgatg ccgttgttac ggtcagtagt ttcaaaacag gtcatcgcgc gcaggccaac
    30421 ttttaaaaat gcgtcgcgca atgtggagag cgacccgccg atatacgccg gagaggcatg
    30481 gtgatcgata accgatgtac atccgctctt aatcgcttcc agggaacaaa tcagtccgct
    30541 gtaatagagc gactcttcat caagggcgcg atcgagccgc caccagagat ttttcagcgt
    30601 tgagatgaaa tccgggcaag gggcgatgtt tgccataatt ccgcgggaaa gccccgagta
    30661 aaaatggttg tgcgagcaga caatccctgg catcacaatc cggccatgca tctctttgaa
    30721 gctggcgtcg gggtagcgtt gcgtcagggc atcgccgata gcgacaatca catcgttttc
    30781 gatggcgata tcaacgcctt cctgcacttt tgccgggtgt agctgtaccg cagtgacatt
    30841 cttcagaatc aacatgatta cacctccacg cggcccagca gataatgatg atgctggtgg
    30901 acatggctga tgatgcggca catatcgttc agctccggtg gtacgttgtt aaactgacct
    30961 ttgctgtcga tgtttaacac ccagctttgg ttattcagac gtactcgtac ccggcaatct
    31021 tccacaagga agcctgggtt gctgctgtta tcaaagtctt gcgccaggct gaagacggtg
    31081 attttgtctt tgtacggttt accgttccac ggacagaact gagcgcagtt gccgcattcg
    31141 ttacagtaag cgtcgaggtg cagcgtctgg aaacggttct ggaagcctgg gaccgcaatg
    31201 gagacgttgg cgcggttcgg gcagacatcc acacacttgc tgcaaacgta gttacattcg
    31261 aggcagcgag cggcttcctg ggcgacaaac gcgtcacgat cgtcactgtt caccagcgtg
    31321 atagagatat cgcctttacg ttgatagatt tccgctggat tgacgttgtt ccagtattta
    31381 tcgttctggt gggaacggat attttcccga ctaaggatgg catcggtcgc ccgacgcgcg
    31441 gttccgacag cagcgacaat ggaggatggt ccgcgctgta cgtcgccgat cataaagacg
    31501 tcagtcagac gagtttcgcc attatggtcg acgtctggcc agccgttttt gtccagcggc
    31561 acgcccatcg cattcagggc ttcagtatcc tgctgttcac caatggcggt gatcaggctg
    31621 tctacaagca gtgttactgt ttcattggtt tcaaccggac gacgacgacc tttctcatcc
    31681 ggttcgccaa gcgacataac gcgcaaggtt aaggtgccat cagcatcgaa acgttccgga
    31741 ttattcagga aacggaactc tacgccgtcg tgcaacgctt cttcatactc ttcgcgccat
    31801 gcgggcatct cttgtagtga acgacggtaa acgatcgttg ctttttctac gcctggaacg
    31861 cgtaacgccg cacgagcgca gtccattgcg gtgttacccg ccccgacaac gaccacatgt
    31921 ttgcccagct tgagcgctgt acccttgttg tattcacgca ggaaggggag tgatttccag
    31981 acattttggt tgtcgcccgc cagtttcaca ccgctatttt tatcagtgcc ggtggcaatc
    32041 agaacatagt ggaagccctg attttttaac tgctcaatgg ttaaatcggg tgagcagcca
    32101 tactcaaatt tcacgccgtg agcggcaaca aaatcgatat cgtgctgaat taactctgca
    32161 ggaatacgga actgaggaat gatatttttc accacgccgc ccgcattggc ttcgcgttca
    32221 aacagcgtaa ccggatggcc cgctctggca aggaagtaac ctgctgccag acccgccgga
    32281 cctgcaccaa tcacggcaac cggatggcgt gaaccagaac cggctggttt gtgccagcgt
    32341 tgcttatatt catcccaacc tttttccagc gcgacttttt tcagttcgcg gatattcagc
    32401 gcactgtcgt aatccaggcg ggtacagttg tattggcact ggtgatcgca aatatgaccg
    32461 gtaatggcgg gcagagcgtt gcgttggtag atgagttcca gcgcgtcggc atagcggtgt
    32521 tcgccaagca gacggatgta ttccggaata tcttgcttga tagcgcaggc agtaacacag
    32581 ggggcaacgt agcagtcggt cagcggcagg tcttctgcca cttcaatacg ctcttctggc
    32641 ttccagtgtt tctgggtgta ttccatggtt agcgcgtctg ctgccagtct gttcagtcgt
    32701 tcgacgtcaa catggtcaag tccccaggcg tcggagcctt ccagctcgcg catgcaggca
    32761 cttaaacgca gatagccacc aggtttcagc aggtcggttg ccatagtaat agggcgaata
    32821 cctgtatcaa aaatatcgcg gatagtcagc tgactggcac caccggaata agaaatgggc
    32881 agtttgccgt caaaggcgcg agagagaact gctgcaacat tgatggagag cgggaacagc
    32941 gcacggcctg acatatacat ctcttcacca ggcagtgcgc ctttattgtt gatggtgccg
    33001 agagtgttag tcagttttac gccaaagccg agtgattttt cttttgccag tgccatcagg
    33061 cgttccagca tttccagtgc ttgcgtcagc ttgaggtcgt gatcaaatga ctcttctttt
    33121 aagcctatgt aaccgaaacc gcagacatcg aggatctcac gaacacgcgc gtaccccagt
    33181 aaggtcgggt taagtttcac aaaggtgttg agcccttttt cttccagcat gtagcggcaa
    33241 atggcttcga tttcatgcgg aggacagccg tgcatggtgg agagggtgac gccatgcacc
    33301 atactggtgg ggatgcgagc gggtaaggct tgcaagcttt cgcgtttttc ctgcaatccg
    33361 tggcgagcta aaaatgcgtc atcctggagt aatttattca gcgtatcgcg atattgagcg
    33421 aatttcggat ggtcagatgc gtccatcata ttgtcgatga actgttgcat cggcggttgc
    33481 ttaatacctt cgaggttgta accgacgctc atattaaaga tgaacgattt accggaatca
    33541 gaaggctgga acatcgcttc gagaaggtgc agggcaaacc aggctttgag gtattcatcc
    33601 caggctttaa gcagggtaaa ttcggtagac cattcggtgt taaagcactc gtcttcggca
    33661 tcgatacagg gcttttccag ctccaggcgg tcaagaattt ggacggtttt tagttcgatg
    33721 aagcgtccgc cagtcagcca ggaagtgaca atattttgcg cgagctgcgt gtgcgggcca
    33781 gcggcagggc cgacgggagt ggcacaggtt tcaccgaaga cgctaacagt tttacctttt
    33841 acaggtgagt aaaactgttg ctcgggaata ccaaagattg agcgttgttg ttggtattca
    33901 tcaaatatgc gcgtcaaaag ttcctcaaac ggaatgggac gcataatatc ccccataacc
    33961 ctctccttag cttatacaga tatcattgag tgaccggtgt tgagaaatcc ggtggtttag
    34021 ggggatagag caacaatcgc accactcttg ccaggtttgg cgggaaatat aaaaaaatgt
    34081 gaggcggctc gaaacttaag ggctttttgt gaatgaactc acaggtgcta aatgagaatt
    34141 aaaatcactc ctgaaaatta ttattgccag cccatttatc aatttttctc ttttctcaaa
    34201 atcagtgatt ccttatcatt atgataatga gtgccggaag gattaaatat ttgaacgcaa
    34261 tcgtttaact acgttttcta aatttcagta tacctttcct tcgctgtaat aaagtcgtcc
    34321 ggagtatcaa tatctaaaat tatttctgca ttttctattt cgacggaata atgatctccc
    34381 ataaggagtg cctgacgcat attggtgaca ttgggtcgtt ggattgcctg tatcaagcat
    34441 ggttttgaca ctaaaatcgg atggccgggg atgccattat ggagtggcag tattgcgcca
    34501 tcatttcgta acgaccagat ttttctaaaa atatcgatag tgagggttgg catgtcaccg
    34561 tgggtgagaa aacaatgttc tgtttgcact gcgggtactg cggccttgac ggatgttagt
    34621 aaaccctgcg cataatctgg gttgtgaata atagtgatat tgctctggtt cgcataacgt
    34681 tcgtgcagtt cattaccacg atagccggtg actaaaataa ttcggctaca aaactgcaac
    34741 gcatttttga tacttgtatc aagagttgtt ccctgttccc agggtaacat cattttccat
    34801 tgccccattc ttgatgataa tccggcagcg gtaattatac agtcgatggc tgacatagtg
    34861 gttccattat ttataggggc taataacgcc tgataattgc gttacttcct gatggattaa
    34921 gttggggata atttatccca gagaggtcat aaagactcgt gaaaagtata gttgacccat
    34981 cggcattagt cattgattta ggtgcgcaga aacgtcccac tgtgatttct gttgttggtg
    35041 ctggggggaa aaccagcttg cttttttggc tggcagaact gctccaggct agcggcaggc
    35101 gtgtgttaat cacgacgacg acacatatgt ttatgccaac gtctcactgg cccgtggttt
    35161 tctgtcgtga tcccgccatg cttcctcatg cgtctcttac atcccccatt tcattttgtt
    35221 ttcacagctg gaaagcgaac cagggaaaag tgcagggatt tacgccagaa gcgattgatg
    35281 cactggtgca acgaccagag tgtgacgtaa ttctcattga agcagatggc tcgcgtggaa
    35341 tgccgttaaa agcgcctgat gagcacgaac cttgcatacc caaaagcagt tgctgcgtga
    35401 ttgctgtgat gggaggacat actttgggcg cgaaagtgag cacagaaaat gtccatcgct
    35461 ggtcgcagtt tgctgatatt acggggttaa cacctgatgc aaccttgcaa ctgagcgatc
    35521 tcgttgcgct ggttcgccac cctcaggggg cgtttaaaaa cgtaccgcaa ggttgtcggc
    35581 gagtctggtt cattaaccgt ttttctcaat gtgagaatgc gattgcacaa agcgagctcc
    35641 ttcaaccgct gcaacaacac gacgtagaag caatctggct gggcgatata caagaacatc
    35701 ctgcaatcgc gcgcagattt gtgaattagc ggcccgggta ttccgggtta ctgaaaggat
    35761 atccattgag gttggtatga atattttcac agaggctgca aaactcgaag agcaaaattg
    35821 tccgtttgcg atggcgcaaa ttgttgatag ccgaggctcg actccccgcc attctgcaca
    35881 aatgttagtg cgcgccgatg gttctatcgt cggtacaatt ggtggcggaa tggttgagcg
    35941 gaaggtgatt gaagagtcac ttcaggcatt gcaggaacgt aagccgcgat tattccatgg
    36001 acgtatggct cgtaacggtg cggatgctgt cgggtcagat tgtggaggtg caatgtcagt
    36061 atttatcagc gtccatggta tgcgtccacg tctggtgttg attggcgcgg ggcatgtcaa
    36121 ccgggcgata gcccagagtg ccgcgctatt aggatttgat attgccgttg ccgatattta
    36181 tcgcgaaagc ctcaatcctg aactattccc accatcaacc acgcttctcc atgctgagtc
    36241 gtttggtgcg gcagtggaag cactggatat tcgccctgat aattttgtcc tgattgccac
    36301 gaataatcag gatcgtgaag ccctcgacaa actcattgaa cagcccattg catggttggg
    36361 gttgctggca agtcgccgca aggttcagct tttcctgcgt caattgcgtg agaaaggcgt
    36421 ggctgaagaa catattgccc gtttacatgc gcccgttggt tacaacatag gtgcggaaac
    36481 gccgcaggag atcgccatca gcgtgctggc agaaatatta caggtgaaaa ataacgcgcc
    36541 gggtgggctg atgatgaaac cttctcatcc ttccggacac cagctggtgg tgattcgcgg
    36601 tgcgggagat atcgccagtg gtgtggcgct acgtctgtat catgcgggtt ttaaagtgat
    36661 catgctggaa gtggaaaaac cgacagtgat tcgttgtacc gtggcgtttg cccaggccgt
    36721 gttcgatggc gaaatgacgg tcgaaggcgt cactgctcgc ctggcaacca gctctgcgga
    36781 agcgatgaaa cttaccgaac gcggattcat ccctgtgatg gtagatcccg cctgttcatt
    36841 gcttgatgaa ctgaaaccgc tttgcgtggt ggacgctatt ctggcgaaac agaatttggg
    36901 aacgcgggca gatatggcac cagtaacaat cgcgcttggg ccgggcttta ctgcagggaa
    36961 ggattgtcat gcggtaattg aaacaaatcg cgggcactgg ctcggtcagg tgatttactc
    37021 tggttgtgcg caggagaata ccggtgttcc tggcaatatt atggggcata ccacccgacg
    37081 ggtgatccgt gctcctgctg caggcattat gcgatccaac gtgaaattag gcgatctggt
    37141 gaaagagggc gatgtgattg cctggattgg tgagcatgaa attaaagcac cgttgacggg
    37201 gatggtgcgt ggcttgttga acgacggcct ggcagtggtt ggtggtttta aaattggtga
    37261 tatcgatcct cgtggtgaaa ccgctgattt caccagcgtt tctgataaag cccgggcgat
    37321 tggcggcggc gtacttgagg cgttaatgat gttgatgcat cagggcgtga aagcgacaaa
    37381 agaagtgctg gaagtggctt aaatgaaaat ggccggatgg tattacccac ccggcctgag
    37441 actgatgaca aacgccaaat tgcctgatgc gctacgctta tcaggcctac aagtgcattg
    37501 caatctattg aatttgcgat gttttgtagg acggataagg cgctcgcgcc gcatccggca
    37561 tcaacaaagc gcacttactt gacaatcttt ggccggatgg tattacccac ccggccttta
    37621 aattactgac aaatcaccgt tcctgttttg ccttcaatcc cttctttcgc tttgctcaat
    37681 acggtaatca gtgcttcacg acctgcgcga gagcgagcaa acgaggcggc agcttccact
    37741 ttaggcagca tagaaccttt cgcaaaatga ccttcttcga taaagcgttc agcatcactt
    37801 aatgacaggc gatcgagcca ctgttcattc tctttaccga agttaatggc cactttttct
    37861 accgcagtca gaatgatcag catatcggca tcaatcattt ctgctaaacg ggcgctggcc
    37921 cagtctttat cgataaccgc gctggcacca cgcagatggt taccttcacg aataacaggg
    37981 ataccaccac cgccaacggt gatcaccacc tgacctgcat ctaccagagc tttaaccgtt
    38041 tctttttcaa tgatatcaac cggttttggc gaggcaacaa cacggcgata gccgcgacca
    38101 gcatcttctt tcagagtgta accctgtttt gtcagttgct ctgcttcttg ctcggtaaag
    38161 aacgaaccga tcggcttggt cgggttgagg aatgccggat cgttagcgtc gacttcgacc
    38221 tgagtaacca gcgttgctac aggtttatta atgccccgag aaagcagttc ttctcttaaa
    38281 gcgttttgta gatcgtaacc aatataaccc tggcttaatg caacacagac agacatcggc
    38341 agcattggcg agtgcgcttc agtttttgcg gcagcttcaa aggcctggtt aatcatgccc
    38401 acctgcggcc cgttaccatg agtgacgata acttcatgtc cctgggcaat taaatcaaca
    38461 attgcctgag aagtaatttt taccgctttc atttgcccgg ccaggtcatc gcccagcgca
    38521 ttcccgccca gggcgagaac aattttctta ctcatttttc ctcactcatc attcttgatt
    38581 gtgtatagat ccctggcagt ggttagagca cgggagggac aaacggtttg cgtcgcagga
    38641 aacgacctcg cccggctttg cctgtaaagg tgccgtcaca gaaaatcgtt tcaccacggg
    38701 ataaggttct gacaatcgcg ccctgacagg taaaaccctc ccatggcgag tagtcggcgt
    38761 tgtcgtggag atggcgatgc tgaatttgtt ggctctgacg tgggtcgata atcaccacgt
    38821 cgccgtcgga accgggcgct aataatcctt tttgcggcca caggccaaac agcctggcgg
    38881 gcattgcgct ggttaattca acaaagcgtt ccggtgttat acgtcccgtc atcacgccac
    38941 tggaaaacag taactgcatg cggttttcca caccgggtaa gccatttggg cagcgactga
    39001 aatcgccttt agaaatttgc aggcgttgag ccatcgagaa ggtgcagtga tcggttgcca
    39061 ccacgtcaat cgcaccatcg ctgatgccac accacagttt gtcctgctcg cgtacgttac
    39121 gcagcggtgg gctaagaatg aacttcatgc catcttctgt atcgtaactg cgttcgtcca
    39181 acaggagata ttgtgggcag gtttcaaccc agactggctg gtgattcgca cgggcaagac
    39241 gcagataatc cagacctaag ccgttagaca ggtgcacgat atagagcggg gcgttaccgg
    39301 caatttgtgc caggttaatc atgcgggcga tggcttccgc ttcgcattcc agagggcgac
    39361 tcaaggcgtg atagcgcggc gcggttaacc ctgcggcgat aaactccgcc cgcttgctgg
    39421 cgatagccgc atcattttcc gggtgcacgg tggtcagcgc gccggattca tgcagacggc
    39481 gtaatgcctg caaaacctcg tcatcgttga gtttgtattg ataggttaaa tagagtttaa
    39541 aactgctcag tccttcctcg accatcatcg ggatttcgtc gaggattgcg tgattaatgt
    39601 gctggatcac accgtgaaag ctgtaatcga tgaccgcttt atgggcggca taaccacgat
    39661 aaacctccag ttgatggcgt aaccgacagc cgtttgggcc aaatcccata tggtcaataa
    39721 tggttgttgt accgccacac gcagctgcgc gggtaccggt aaaaaaatca tcacaactgc
    39781 gcgcgatgcc gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt
    39841 aacagccagt ggcatcaatt tcttcatacg ggagctgcgg cgaaatattg ttgcccaact
    39901 ggcgaacaat cccgctttca atcagcaaat cctgtttggc ttgtccatct gcgttaacga
    39961 cagtgccgtt tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga
    40021 ggggaataag tgaagagtaa ttattcggtt gccagccagc ttaacgggat tgcagcgtac
    40081 atcgcggcac aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca
    40141 ggttctttac ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg
    40201 ccgttagttg agaaggtcca cttatcaaca accggcgctt tgccaaacag cccttcgtag
    40261 gcattcacca gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag
    40321 cattcggttg ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca
    40381 accgcgttag ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag
    40441 gtttcgcccc aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa
    40501 cggcttgggg atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg
    40561 tcataaccca gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa
    40621 atggcgttgt caccgcgttc tggcgcagaa ccgtggcagc taacaccctg aacatcaata
    40681 cgaatttcca tacgaccgcg ctgaccacgg tatacctggc agtcggttgg ttcggtactg
    40741 accacaaatt ccgggcgaat gccggattgt tcaataatgt actgccagca cagaccgtcg
    40801 cagtcttctt cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg
    40861 tctttaatga ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta
    40921 ccgcgaccac cgattagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt
    40981 ttgatgttgc caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg
    41041 tggccgatat aaccgagaac gttgcccatc gggtcgattt caactttatc gaagccgact
    41101 ttttccatct cttctttaat acgatgtact acgcgttttt cgtcgcagct ttcactggga
    41161 atagcaacca tgtcgcgcag gaagcgagtc atgtccgcct ggtaatcttt tgctttttca
    41221 agaatcagtt tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg
    41281 ttttgcaggg agtagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag
    41341 acaacttcgc ggtagtgctt cacgtcggtg tcgccttcag tactgataac cagcactacg
    41401 gcatctttgt tcagcgccag tttttccatc aggctttgac gttgcgggtg ataatgaacc
    41461 gctgcgagaa cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg
    41521 ccgtacggat tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg
    41581 aattgggtgg cgcagttacg taggatttcc cagcccagcg ggttaggttc gccacaggcc
    41641 aggcccgcca tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg
    41701 gagcgataaa tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg
    41761 ctgtagacgt cgaccagata acccagcaca ccaccggcca ttgctccgac accggcttgc
    41821 agcagaacgt gcgtcggggt tacgcccatt tcacgcattt gctcgacggc ttcatctgcc
    41881 agggttgcgt agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg
    41941 tcctgtacca cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg
    42001 tcatagttca tatctgtgac gatgcactcg gcaccgaggt tcagaatggc gtcaacgcgt
    42061 tcctgagcag aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc
    42121 cacgccacac cgcgcccgtg gttgccgtcg gtggtggtcg cgaaagtcat tttttcgccg
    42181 atggtatttt tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac
    42241 aataattgag cgatggcgta cgcaccgcca agcattttga aggcgttcag accgaatcgt
    42301 tttgattcgt ctttgacgag aattttcttc acaccaaaaa ggtttgcgag atcgtccagc
    42361 gcacaaagcg gtgttgggcg ataaccagct attttctggt ggaactggcg cgccagtttg
    42421 gcttggcttt gcgaaaagag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata
    42481 tcaatcttca atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc
    42541 gaccagctga tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc
    42601 tcgagcagtg cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa
    42661 ggcttccagc tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc
    42721 acttcacctt ctttacagga tacgccgctg atatcagctg gcaggcagtg catgtacagg
    42781 gcttcgccat cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc
    42841 gcgttctgtg ccagacactg tttttccagt gcttttaagc cttcgtgatc gttcgcacgc
    42901 agcaattcag tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg
    42961 tctgcgtctt tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct
    43021 ttagcattgt ttttcgccac ttcaaccaca tccgggatca ggtcgtagcc ttccggatgg
    43081 gccagggtga catccatacc gaagcgagtc atcagaccga tgatgccttg tggtacagag
    43141 agcggtttgc catagcttgg agagtaggcc caggtcatgg cgattttttt acctttcagg
    43201 ttttccagtg aaccaaagtg ttcacgtaac cacgcgaggt cagccattga ctgagtcggg
    43261 tggtcaatat cgcattgcag gttcactaaa gccggacgct gtggcagtac accctgcttg
    43321 taaccgtcat caagtgcagc gccaacttca cgcatatagg cgttgcctgc gcccagatac
    43381 atatcgtcgc gaataccaat agcgtcggcg cagaaggaga tcatattggc ggtttcacgc
    43441 acggtttcgc cgtgagcgat ttgtgatttg ccttcgtcga gatcttgttg tgcaaggccg
    43501 agcaggttaa gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg
    43561 gaaataccta atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt
    43621 aatgctgcgg caacgtccag tacttgtttc agttcatctg gcgtctgttc ccacgttaac
    43681 aaaaaatctt tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta
    43741 acagttttca tcataaagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt
    43801 gacttgccaa agtgattggt gttttcacag ctatacctgg gactaacaat aagtgtgcca
    43861 gaattatccg cgcggctgca aaagactaat ttgacggata atgtgtgggg ttgatcacaa
    43921 attgatgcca gcttctggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt
    43981 tatttatttg ctgctaagta tcagaaataa gataaagggg caggctatct ctcttcagtt
    44041 tgataaactc accgccgtaa ttctcatatt gatatagaaa tgtgatatct cttccataat
    44101 ttcattagaa aatgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat
    44161 atcatcatga tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa
    44221 ggtaaagcta tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt
    44281 cagcgttttg ccagaatgct tgccagcgtt ttgcagcttg aggttgagat cgttgatgaa
    44341 aacttgtgtc gcgttgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc
    44401 ggcaactcac gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag
    44461 tctcgcttcg atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca
    44521 tttctgggta cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc
    44581 gttacccacg agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt
    44641 cgccatatat ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac
    44701 atcagtaaaa tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat
    44761 gatgaaagtc gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtacaa
    44821 aataatatta ttgggaaacc tatccgtttc agaccattaa catttgagag taattttact
    44881 catggacata tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa
    44941 ttgcataaca ttcagggccg acaattattt ttaatggcat ttcaccaatc gcataccagt
    45001 ttttctgtag caaatgcacc tgatgaacca catattgaac aattggttgg cgagtgccgt
    45061 gttatgcggc aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg
    45121 gtggttggtg aaagcggcac gggtaaagaa gtcgtcgccc gagcaatcca taagttgagc
    45181 ggaagacgga ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg
    45241 gaaagcgaac tgttcggtta tgttaaaggc gcatttactg gcgcttctgc caacggtaaa
    45301 acagggttga ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg
    45361 ccattaatgt tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt
    45421 ggtgccagta gcccaataca agtcgacatt cgcatcattt ctgcaactaa tcagaatttg
    45481 gcccagttca ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc
    45541 ccgataactc tgccaccgct gcgtgaacgt caggaagata ttgaactatt ggtgcattac
    45601 tttttacatc tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc
    45661 gtcgaaatat tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg
    45721 gaatatctgg ttaacgtggt tccttcaggt gaagttatcg acagcacgct attgccgcca
    45781 aatctgctga ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac
    45841 ctgtcactcg atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc
    45901 gaggcgcttt cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt
    45961 gctacgctct atcgcaagat taagaaatat gagttgttaa acacataacc tcagtgaatc
    46021 cggcctgaat tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt
    46081 tacaatcatc tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc
    46141 cgtaatggtt aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag
    46201 gccaggcgta caaaacccgc actgcactgc gccggatttc gcataagcct gctgaacatg
    46261 agaaagtttt ccgcctttcg cttcaccttc cagcgtgcgg atctcttttc cttcagccca
    46321 ggcggcaagg tataagcaac tgtctattgc tgtgccgtcg accaacactg tacaggcacc
    46381 acattcaccc acgcagcacc cttgtttgac acttagcagt ccttgttcgc ggagtaattc
    46441 cgagagcggc gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc
    46501 gatggtaatt gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg
    46561 ctaatcactt ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc
    46621 catgaagaac gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc
    46681 aggtttaatg gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc
    46741 gcggcaacac caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt
    46801 gcggcgcagc caatcgttga aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg
    46861 ctgcccgcgt gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc
    46921 tcaagagaca ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg
    46981 cgcggggagt ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg
    47041 ctggtggcac cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca
    47101 gcaatggacg tggccgcagc acataacgcc gggagatgac gttgagttat aggatcttct
    47161 attagctggg taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta
    47221 attccccgca gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga
    47281 tggtggagct gaatcagtac gtcagtgcca ccggcgagca gtttggcctg cgggttgtca
    47341 gccagcaggt tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac
    47401 atgatgttat cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt
    47461 cagcggcagt gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc
    47521 ggcaacagga attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc
    47581 attgatttcg acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta
    47641 atccagcaga ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa
    47701 tagcgcccag ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg
    47761 attaagaata tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag
    47821 cgcaatatcg accgtcagat caacaaaggt acagccaaac gccggtgggt tagtggtggt
    47881 tttgatggag ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat
    47941 cgccaaatct tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc
    48001 ttttatcagg gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat
    48061 tttctctttt aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg
    48121 tgaggcaaat gcgccgggat cgaacggcgt aacgtcggta tcttgagttg aaataacgcg
    48181 aacgtcgctg accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc
    48241 accctgaccg atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag
    48301 aaggcgcgcg cctgctattt ctacgccgac aggccaggtg ttagaggtgt agctaaaaca
    48361 ggcgacgcca acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt
    48421 ttcccattca aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat
    48481 acgtttgccc gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc
    48541 aacaggatca atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac
    48601 gacttgtggc gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt
    48661 ctttgaactg taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcccccccgc
    48721 agaagcgatg gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact
    48781 ataacctttc aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg taaaagcgtg
    48841 gcgggtacgg gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc
    48901 gccaagcttg ctggtcagga atgccgccat tggctcttcc agtacatcct gtttattacc
    48961 aaaaccgcca ccgacaaatg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc
    49021 ctgaccaacc acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaatttg
    49081 cgagtcatcc tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac
    49141 gggggtctga tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt
    49201 acccgtcgac atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc
    49261 ttctgccagc gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac
    49321 caattgcgcc gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc
    49381 accatgatga cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc
    49441 gtcaagtgtc caggcatgcc cagctgtagc gaatggaata tcaggcacat cttcccaggt
    49501 aaaaatcgcc agtacgcccg gcaaacttct ggcttgttca tcattaatac ttacggcata
    49561 accatgtgcg atagggctac gtacatattt cgcataacac atgcccgcca taacataatc
    49621 gtcagtatat cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc
    49681 acccgtagcg gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc
    49741 ttatggcaaa gatttagtct aagcaggcaa gatttacgcc agaaacgacg ataagcgtta
    49801 ggggaggtag gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct
    49861 gaataggggc agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattggg
    49921 attattacgc atcaggatgg gaactagggt aaaaagtcag gaagttcgat acctctacag
    49981 cgaatatttt gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac
    50041 cacgtttttc cagaagcaag agattgggta tcaagaggct ggctgctatg ataaggcgtc
    50101 ttgtttttta agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt
    50161 atcgtgatgt tgttatcggt tggactgctt ttggcgggct gttcgggtag caaatcatcc
    50221 gatacaggaa cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt
    50281 atttcgcgca ccacgggaac cagcgtaaaa gaactggcgc gactgaacgg catttccccc
    50341 ccttacacca ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt
    50401 acacgtaaat caaccgccaa atcaacgacc aaaaccgcat cggttacacc gtcatcagcg
    50461 gtaccgaaat catcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg
    50521 aaagttatca tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct
    50581 ccacggggta cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag
    50641 ctgcgtggct acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac
    50701 gcccataatg acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc
    50761 gccactatgg ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt
    50821 gcaacggcaa ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga
    50881 tggcgaatta atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa
    50941 tgccttacgc atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct
    51001 atacgagggt tcgattccct tcgcccgctc caatttatct tcgccgtaat acttccccat
    51061 tttgttgtaa ataaaaagcg tttacgcaat gcaatccttt tgtaacaacc caggtaaaac
    51121 accttatttt caagttgtta aataagtggt tatctacgct tttaacaaac gattgatgga
    51181 atattttatg aaagggaaat ctgcattaac tcttctgctc gctggtatat tttcgtgcgg
    51241 cacatgccag gcgacagggg cagaagtgac gagtgaatct gtatttaaca ttcttaattc
    51301 cacgggggca gcaacagata aaagttattt atcgttaaat ccagataagt atcctaatta
    51361 tcgtcttctg attcactcag ccaaattaca aaatgaaata aagagccact ataccaaaga
    51421 cgaaattcaa ggattgctaa cattaacaga aaacacacga aagttaacgt tgacggagaa
    51481 accctgggga acgtttattc ttgcttctac tttcgaagat gacaaaacag cggcagaaac
    51541 ccactatgat gctgtctggt tgagggatag tttatggggc tatatggcat tagtatcaga
    51601 tcagggaaat agtgtagccg caaaaaaggt tctgctcacc ctgtgggatt atatgtctac
    51661 gcccgaccag attaagcgga tgcaggacgt cattagcaac ccaaaacgac tggatggaat
    51721 tcccgtgcaa atgaatgctg tgcatatccg atttgacagc aactcccccg tgatggcaga
    51781 tgtgcaggaa gaaggcaagc cgcagctgtg gaaccataaa caaaatgatg ctttaggtct
    51841 ttaccttgat ctccttatac aagcaatcaa cactggtact attaacgctg aagattggca
    51901 aaaaggtgac cgattaaagt cggttgcgct gctgattgct tatctggata aagccaattt
    51961 ctatgttatg gaagattcct gagcatggga agaagatgcc cgattaaaca catcttccgt
    52021 tgcactggtg acctctggac tggaacgttt gtcaaaccta ctctcgaaga aagactctgt
    52081 ttttgtctct gatttactac gcgaagctaa agtcaatgaa ctggatgaaa ctctttcaac
    52141 aacgaggttg aaccacctca tagataaagg ttatgaaaga ataacgttac agcttgattt
    52201 agggggagaa tccccaggtt atctggaaaa agataaacat tacagagaag ccgatgcg
//
LOCUS       DAJOSY010000017        51283 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJOSY010000017 DAJOSY010000000
VERSION     DAJOSY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.017"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            57..1496
                     /gene="norV"
                     /locus_tag="OHN27_001116"
     CDS             57..1496
                     /gene="norV"
                     /locus_tag="OHN27_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCQ2285598.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNSVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            1493..2626
                     /gene="norW"
                     /locus_tag="OHN27_001117"
     CDS             1493..2626
                     /gene="norW"
                     /locus_tag="OHN27_001117"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCQ2285599.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DISHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(2754..5006)
                     /gene="hypF"
                     /locus_tag="OHN27_001118"
     CDS             complement(2754..5006)
                     /gene="hypF"
                     /locus_tag="OHN27_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCQ2285600.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLDLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRFFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(5159..5686)
                     /gene="hydN"
                     /locus_tag="OHN27_001119"
     CDS             complement(5159..5686)
                     /gene="hydN"
                     /locus_tag="OHN27_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCQ2285601.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(5835..6845)
                     /gene="ascG"
                     /locus_tag="OHN27_001120"
     CDS             complement(5835..6845)
                     /gene="ascG"
                     /locus_tag="OHN27_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCQ2285602.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINARHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEQLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIAL
                     SR"
     gene            7105..8562
                     /gene="ascF"
                     /locus_tag="OHN27_001121"
     CDS             7105..8562
                     /gene="ascF"
                     /locus_tag="OHN27_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCQ2285603.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            8571..9995
                     /gene="ascB"
                     /locus_tag="OHN27_001122"
     CDS             8571..9995
                     /gene="ascB"
                     /locus_tag="OHN27_001122"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCQ2285604.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPV
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     INYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(10153..10623)
                     /gene="hycI"
                     /locus_tag="OHN27_001123"
     CDS             complement(10153..10623)
                     /gene="hycI"
                     /locus_tag="OHN27_001123"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCQ2285605.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(10616..11026)
                     /gene="hycH"
                     /locus_tag="OHN27_001124"
     CDS             complement(10616..11026)
                     /gene="hycH"
                     /locus_tag="OHN27_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCQ2285606.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(11023..11790)
                     /gene="hycG"
                     /locus_tag="OHN27_001125"
     CDS             complement(11023..11790)
                     /gene="hycG"
                     /locus_tag="OHN27_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCQ2285607.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(11790..12332)
                     /gene="hycF"
                     /locus_tag="OHN27_001126"
     CDS             complement(11790..12332)
                     /gene="hycF"
                     /locus_tag="OHN27_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCQ2285608.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(12342..14051)
                     /gene="hycE"
                     /locus_tag="OHN27_001127"
     CDS             complement(12342..14051)
                     /gene="hycE"
                     /locus_tag="OHN27_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCQ2285609.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFHVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(14069..14992)
                     /gene="hycD"
                     /locus_tag="OHN27_001128"
     CDS             complement(14069..14992)
                     /gene="hycD"
                     /locus_tag="OHN27_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCQ2285610.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(14995..16821)
                     /gene="hycC"
                     /locus_tag="OHN27_001129"
     CDS             complement(14995..16821)
                     /gene="hycC"
                     /locus_tag="OHN27_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCQ2285611.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(16818..17429)
                     /gene="hycB"
                     /locus_tag="OHN27_001130"
     CDS             complement(16818..17429)
                     /gene="hycB"
                     /locus_tag="OHN27_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCQ2285612.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(17554..18015)
                     /gene="hycA"
                     /locus_tag="OHN27_001131"
     CDS             complement(17554..18015)
                     /gene="hycA"
                     /locus_tag="OHN27_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCQ2285613.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            18227..18577
                     /gene="hypA"
                     /locus_tag="OHN27_001132"
     CDS             18227..18577
                     /gene="hypA"
                     /locus_tag="OHN27_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCQ2285614.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            18581..19453
                     /gene="hypB"
                     /locus_tag="OHN27_001133"
     CDS             18581..19453
                     /gene="hypB"
                     /locus_tag="OHN27_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCQ2285615.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            19444..19716
                     /gene="hypC"
                     /locus_tag="OHN27_001134"
     CDS             19444..19716
                     /gene="hypC"
                     /locus_tag="OHN27_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCQ2285616.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            19716..20837
                     /gene="hypD"
                     /locus_tag="OHN27_001135"
     CDS             19716..20837
                     /gene="hypD"
                     /locus_tag="OHN27_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCQ2285617.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            20834..21844
                     /gene="hypE"
                     /locus_tag="OHN27_001136"
     CDS             20834..21844
                     /gene="hypE"
                     /locus_tag="OHN27_001136"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCQ2285618.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            21918..23996
                     /gene="flhA"
                     /locus_tag="OHN27_001137"
     CDS             21918..23996
                     /gene="flhA"
                     /locus_tag="OHN27_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCQ2285619.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(24033..24386)
                     /locus_tag="OHN27_001138"
     CDS             complement(24033..24386)
                     /locus_tag="OHN27_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCQ2285620.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPHE"
     gene            complement(24418..24597)
                     /locus_tag="OHN27_001139"
     CDS             complement(24418..24597)
                     /locus_tag="OHN27_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285621.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            24673..27234
                     /gene="mutS"
                     /locus_tag="OHN27_001140"
     CDS             24673..27234
                     /gene="mutS"
                     /locus_tag="OHN27_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCQ2285622.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            27340..27996
                     /gene="pphB"
                     /locus_tag="OHN27_001141"
     CDS             27340..27996
                     /gene="pphB"
                     /locus_tag="OHN27_001141"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCQ2285623.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPEID
                     LLISVGDNIDRGPESLDVLRLLNQPWFTSVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYAIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPNSGRLSFYKIK
                     "
     gene            complement(28047..28814)
                     /locus_tag="OHN27_001142"
     CDS             complement(28047..28814)
                     /locus_tag="OHN27_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2285624.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALVKQDLSLLVAKNE"
     gene            29010..29918
                     /locus_tag="OHN27_001143"
     CDS             29010..29918
                     /locus_tag="OHN27_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCQ2285625.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            29915..31177
                     /locus_tag="OHN27_001144"
     CDS             29915..31177
                     /locus_tag="OHN27_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCQ2285626.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQARKAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSIETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDDAFFSRAQREFLS"
     gene            31174..31812
                     /locus_tag="OHN27_001145"
     CDS             31174..31812
                     /locus_tag="OHN27_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCQ2285627.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            31817..32593
                     /locus_tag="OHN27_001146"
     CDS             31817..32593
                     /locus_tag="OHN27_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCQ2285628.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYNYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNIRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            32682..34046
                     /locus_tag="OHN27_001147"
     CDS             32682..34046
                     /locus_tag="OHN27_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCQ2285629.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGVVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(34140..35132)
                     /gene="rpoS"
                     /locus_tag="OHN27_001148"
     CDS             complement(34140..35132)
                     /gene="rpoS"
                     /locus_tag="OHN27_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCQ2285630.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(35195..36334)
                     /gene="nlpD"
                     /locus_tag="OHN27_001149"
     CDS             complement(35195..36334)
                     /gene="nlpD"
                     /locus_tag="OHN27_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCQ2285631.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(36474..37100)
                     /gene="pcm"
                     /locus_tag="OHN27_001150"
     CDS             complement(36474..37100)
                     /gene="pcm"
                     /locus_tag="OHN27_001150"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCQ2285632.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(37094..37855)
                     /gene="surE"
                     /locus_tag="OHN27_001151"
     CDS             complement(37094..37855)
                     /gene="surE"
                     /locus_tag="OHN27_001151"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCQ2285633.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(37836..38885)
                     /gene="truD"
                     /locus_tag="OHN27_001152"
     CDS             complement(37836..38885)
                     /gene="truD"
                     /locus_tag="OHN27_001152"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCQ2285634.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(38882..39361)
                     /gene="ispF"
                     /locus_tag="OHN27_001153"
     CDS             complement(38882..39361)
                     /gene="ispF"
                     /locus_tag="OHN27_001153"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCQ2285635.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(39361..40071)
                     /gene="ispD"
                     /locus_tag="OHN27_001154"
     CDS             complement(39361..40071)
                     /gene="ispD"
                     /locus_tag="OHN27_001154"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCQ2285636.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(40090..40401)
                     /gene="ftsB"
                     /locus_tag="OHN27_001155"
     CDS             complement(40090..40401)
                     /gene="ftsB"
                     /locus_tag="OHN27_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCQ2285637.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(40595..40918)
                     /locus_tag="OHN27_001156"
     CDS             complement(40595..40918)
                     /locus_tag="OHN27_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCQ2285638.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(40968..41573)
                     /gene="cysC"
                     /locus_tag="OHN27_001157"
     CDS             complement(40968..41573)
                     /gene="cysC"
                     /locus_tag="OHN27_001157"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCQ2285639.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(41573..43000)
                     /gene="cysN"
                     /locus_tag="OHN27_001158"
     CDS             complement(41573..43000)
                     /gene="cysN"
                     /locus_tag="OHN27_001158"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCQ2285640.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(43002..43910)
                     /gene="cysD"
                     /locus_tag="OHN27_001159"
     CDS             complement(43002..43910)
                     /gene="cysD"
                     /locus_tag="OHN27_001159"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCQ2285641.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            44162..45199
                     /gene="iap"
                     /locus_tag="OHN27_001160"
     CDS             44162..45199
                     /gene="iap"
                     /locus_tag="OHN27_001160"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCQ2285642.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(45598..45882)
                     /gene="cas2"
                     /locus_tag="OHN27_001161"
     CDS             complement(45598..45882)
                     /gene="cas2"
                     /locus_tag="OHN27_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCQ2285643.1"
                     /translation="MSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGVVSARIREMIW
                     EQISGLAEEGNVVMAWATNTESGFEFQTFGVNRRTPVDLDGLRLVSFLPV"
     gene            complement(45884..46801)
                     /gene="cas1e"
                     /locus_tag="OHN27_001162"
     CDS             complement(45884..46801)
                     /gene="cas1e"
                     /locus_tag="OHN27_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCQ2285644.1"
                     /translation="MAWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYALLAKQYGVTW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYD
                     IADIIKFDTVVPKAFEIARRNPGEPDREVRLACRDIFRSSKTLAKLIPLIEDVLAAGE
                     IQPPAPPEDAQPVAIPLPVSLGDAGHRSS"
     gene            complement(46817..47416)
                     /gene="cas6e"
                     /locus_tag="OHN27_001163"
     CDS             complement(46817..47416)
                     /gene="cas6e"
                     /locus_tag="OHN27_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCQ2285645.1"
                     /translation="MYLSKIIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKR
                     NTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKR
                     LDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARIEDVHPISERPQYFSGEGKNGKIQT
                     VCFEGVLTINDAPALIDLLQQGIGPAKSMGCGLLSLAPL"
     gene            complement(47403..48077)
                     /gene="cas5e"
                     /locus_tag="OHN27_001164"
     CDS             complement(47403..48077)
                     /gene="cas5e"
                     /locus_tag="OHN27_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCQ2285646.1"
                     /translation="MKSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACL
                     GIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHE
                     TIQTWREYLCDASFTIAIWLTPQATMVMSELEKAVLKPRYTPYLGRRSCPLTHPLFLA
                     TCQASDPQKALLNYEPVGGDIYSEESVTGHHLKFTARDEPMITLPRQFASREWYVIKG
                     GMDVSQ"
     gene            complement(48080..49171)
                     /gene="cas7e"
                     /locus_tag="OHN27_001165"
     CDS             complement(48080..49171)
                     /gene="cas7e"
                     /locus_tag="OHN27_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCQ2285647.1"
                     /translation="MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSL
                     KRAMRKSDYYAQNIGESSLRTIHLAQLRDVLRQKLGERFERKIIDKTLALLSGKSVDE
                     AEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDI
                     ALSGRMATSGMMSELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQE
                     FSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPA
                     DMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSLQAFNQYWDRVANGYGLNGAAAQFSL
                     SDVDPITGQVQQMPTLEQLKSWVRNNGEA"
     gene            complement(49184..49666)
                     /gene="casB"
                     /locus_tag="OHN27_001166"
     CDS             complement(49184..49666)
                     /gene="casB"
                     /locus_tag="OHN27_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCQ2285648.1"
                     /translation="MADEIDAMALYRAWQQLDNGACAQIRRVSEPDELRDIPAFYRLV
                     QPFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKPEQTTGISLGRALANSGRINERRI
                     FQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA
                     "
     gene            complement(49659..51167)
                     /gene="casA"
                     /locus_tag="OHN27_001167"
     CDS             complement(49659..51167)
                     /gene="casA"
                     /locus_tag="OHN27_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HCQ2285649.1"
                     /translation="MNLLIDNWIPVRPQSGGKVQIINLQSLFCSKNQWRLSLPRDDME
                     LAALALLVCIGQIIAPAKDDVEFRHRIMNPLSEDEFQRLIAPWIDMFYLNHAEHPFMQ
                     TKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
                     GFKSGLRGGTPITTFVRGIDLRSTVLLNVLTIPRLQKQFPNESHTENQPTWIKPVKPN
                     ESVPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGL
                     WPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQF
                     RNIASQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTAL
                     RKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLILDVLANINFSQADEVIADL
                     RDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG"
BASE COUNT        12342 a        13843 c        13103 g        11995 t
ORIGIN      
        1 caatctgcaa ttagcaagac atctttttag aacacgctga ataaattgag gttgctatgt
       61 ctattgtggt gaaaaataac attcattggg ttggtcaacg tgactgggaa gtgcgtgatt
      121 ttcacggcac ggaatataaa acgctgcgcg gcagcagcta caatagctac ctcatccgcg
      181 aagaaaaaaa cgtgctgatc gacaccgtcg accataaatt cagccgcgaa tttgtgcaga
      241 acctgcgtaa tgaaatcgat ctggcggata tcgattacat cgtgattaac catgcagaag
      301 aggaccacgc tggggcgctg accgaactga tggcacaaat tcccgatacg ccgatctact
      361 gtactgccaa cgctatcgac tcgataaatg gtcatcacca tcatccggag tggaatttta
      421 atgtggtgaa aactggcgac acgctggata tcggcaacgg caaacagctc atttttgtcg
      481 aaacaccaat gctgcactgg ccggacagca tgatgactta cctgacaggc gacgcggtgc
      541 tgttcagtaa cgatgctttc ggtcagcact actgcgacga gcatctgttc aacgatgaag
      601 tggatcagac ggagcttttc gagcagtgcc aacgttacta cgccaatatc ctgacgccgt
      661 tcagccgcct ggtaacgccg aaaattaccg agatcctggg ctttaactta ccagtcgata
      721 tgatagccac ttcccacggc gtggtatggc gcgataaccc gacgcaaatt gtcgagctgt
      781 acctgaaatg ggcggctgat tatcaggaag acagaatcac cattttctac gacaccatgt
      841 cgaataacac ccgcatgatg gctgacgcta tcgcccaggg aattgcggaa accgacccac
      901 gcgtggcggt gaaaattttc aacgtcgccc gaagcgataa aaacgaaatc ctgaccaatg
      961 tcttccgctc aaaaggcgtg ctggtcggca cttcgacgat gaataacgtg atgatgccga
     1021 aaatcgccgg gctggtggag gagatgaccg gattacgctt ccgtaacaaa cgcgccagtg
     1081 ctttcggatc tcacggctgg agcggcggtg cggtggatcg tctttccacg cgcctgcagg
     1141 atgcgggttt cgaaatgtcg ctcagcctga aagccaaatg gcgaccagac caggacgctc
     1201 tggagttatg ccgtgaacac ggtcgcgaaa tcgcccgtca gtgggcgctc gcgccgctgc
     1261 cgcagagcac ggtgaattcg gtggttaaag aagaaacctc tgccaccacg acggctgacc
     1321 tcggcccacg gatgcagtgc agcgtctgcc agtggattta cgatccggca aaaggcgagc
     1381 caatgcagga cgttgcgcca ggaacgccgt ggagtgaagt tccggataac ttcctctgcc
     1441 cggaatgctc cctcggcaaa gacgtctttg acgaactggc atcggaggca aaatgagcaa
     1501 cggcattgtg atcattggtt cgggcttcgc cgcccgacaa ctggtgaaaa atattcgcaa
     1561 acaggacgcc actattccat taaccttgat tgccgccgac agcatggatg agtacaacaa
     1621 acctgacatc agccatgtta tcagtcaggg gcaacgtgcc gatgacctta cccgccagac
     1681 ggcgggtgaa tttgccgagc agtttaatct gcacctgttt ccacaaacct gggtgacgga
     1741 tatcgatgcc gaagcccgtg tggtgaaaag ccagaataat cagtggcaat acgacaagct
     1801 agtactggca accggtgcca gtgcctttgt cccgcctgtg cctgggcgtg agttaatgct
     1861 gacgttaaat agtcagcaag agtatcgcgc ctgtgaaacg caactgcggg atgcccgacg
     1921 cgtgttgatt gttggcggtg gtttgattgg tagcgaactg gcgatggatt tttgtcgtgc
     1981 aggcaaagcg gtcacgctaa tcgacaacgc tgccagtatt ctggcgtcgt taatgccacc
     2041 ggaagtaagc agccgcttgc agcatcggtt gacggagatg ggcgttcatc tgctgttgaa
     2101 atctcagtta caggggctgg aaaaaacgga ttctggcatt ctggcaacgc tggaccgcca
     2161 gcgtagcatc gaagtggatg cggtaattgc cgccaccgga ctgcgcccgg aaaccgccct
     2221 ggcacgacgc gccgggctga cgattaatcg cggcgtttgc gtcgatagtt atctgcaaac
     2281 cagtaatacc gatatttacg cgctgggcga ttgcgcggaa attaacggtc aggtattgcc
     2341 gttcctccag ccgattcaac ttagcgcgat ggtgctggca aaaaatcttc tcggcaataa
     2401 cacgccgctg aaactcccgg cgatgctggt gaaaatcaaa acgccggaat taccgctgca
     2461 tctggcaggc gaaacccagc gtcaggattt acgctggcaa attaataccg aacgccaggg
     2521 aatggtggcg cgcggcgttg acgatgctga ccagcttcgc gcctttgtgg tcagtgagga
     2581 tcggatgaaa gaggcatttg gattgttgaa aacattgccg atgtaggtgg gctactgtgc
     2641 ctaaaatgtc ggatgcgacg ctggcgcgtc ttatccgacc tacggggacg catgtgtagg
     2701 ccggataagg cgtttacgcc gcatccggca atggtgtcca aatgcaacac gttttatccg
     2761 ttctggactt cacccgctaa ccaacgcgcc gcagcaataa ccccctgccc cagagacaaa
     2821 ccgccatcac ccgccggtaa actctgtgga aagagcaatg tgaaatcagc gagataatgc
     2881 gccagacgtg cacgcagcaa acggttatga ataaccccgc cgctaaatac cagcgtagtg
     2941 ataccacgca tcgtggcctg ctcacgcatc aacgcggcaa aaccctgcgc cagcgcatca
     3001 tgaaacgccc acgcgcgttg attaaccggt gcctgccagt tcagccactg ctgccagaaa
     3061 gtggcgagat ccagttgatt gtccacccgc ggcattgtca ccggatgcgt cactccgtgg
     3121 catgaggctg cgagcgcctc cagagcacaa gccgcttcac cttcataact taacgtggct
     3181 ggcgcacagc ccagtgccgc cgccactgca tcgaaaaaac gcccacacga tgacgccagc
     3241 ggcgcgttaa ttccacgctc aatggcccgc gccagcacgc tccagttttg ctgttgcaca
     3301 cttgctgttt cagagtaatt ctgccactcc ggcacaaagc gcaggcactg cgccagcagg
     3361 tttcgccacg gctgcttcgc tgccaaatcg ccacccggaa gcgccactgc aggcaagccg
     3421 cccaggtgct cacattcgcg atagttcacc cgcaggcact cgccgcccca caaagcgccg
     3481 ttctccccca taccgatacc gtcgagcgtc aaagcaatga catcaccgcc atccagcggc
     3541 cactgatgct ctgccagaca cgccgctgca tgggcatgat gatgcagtac cgtttgcgtc
     3601 ggcagattca tttcacgcgc ccactggctg gagacatagc ccggatgcgc gtcatgcaca
     3661 acgtattgcg gggtaaaatc gtagatgttt tgcatcaggc gtaacgcttc gcgccactgc
     3721 atctggatgc catcgtcact taaatcgccc agatgctgac tcaacaccgc ttgttcaccg
     3781 cgcaccaggc agaaggtatt tttcagatcc gcgccgagac acagcacagg cggaacattt
     3841 ttaaagcccg gaggcaaagc cagcgcatcc ggcacatacc cccgcgaacg gcgcagcatt
     3901 tcgccgcttt cgcgcaccac cgaatcatcc atccgctgca cgatgtcgcg gttatgtatc
     3961 aagaatccgt cggcaatgcc ctgcaaatcc gccagcgcct gttcgttgct gatagctggt
     4021 ggtttaccgc tcaggttgcc ggaggtcatc accagcgggc attgcagttc ctgtaacagc
     4081 aaatgctgga gcgggttcgc aggcaacatt accccgactt cgttaaggtc aggggcgata
     4141 tcatcacaaa gctcaggaac gtatttttta tccaccagca caatcggcgc ggcgggcgtg
     4201 gtaagcaact ggcgcgcagc gtctggtaaa ccttctgcca ctggcaacat aaccgccagc
     4261 ggtttcgccg ggcgatgttt gcgtgcccgc agtgtcgcca ccgcgttact gttacgtgca
     4321 tcgcaggcaa gatgaaatcc gccaatccct ttgatggcga caatgttgcc catttttaac
     4381 tgtgcgatag ctgcctgtaa tgccgcctct tgttctgcat gttcaccatg acttacccat
     4441 tcaagatgcg ggccacactc cgggcaggcc accggctggg cgtggaagcg acgatcgagc
     4501 gggtcacggt actctttatc acaggccgga cacagcggaa acgccgccat cacggtaaac
     4561 gggcggtcgt aaggcatggc gcgaataatg gtgaagcgcg ggccacagtg agtacagttg
     4621 ataaacggat aacgataacg ccgctcgcct ggggtattca tttcggcaag acaggcagga
     4681 caagtagcgg catcgggaac aatttgcgta ttcatggtgc cgcctgtgct ctggcgtata
     4741 gtgaactcgg tgggcagttg tgaccagata aacggctcac gctcgacgct atcaatacgc
     4801 gccagcggcg ggcagtgctg atgcaattga acaagaaacg tttccgggtc ttccagcagc
     4861 cggacttcta cgccatcgcc gtcattacag acatcgccgt gaagatctaa ttgctgtgcc
     4921 aactgccaga caaacggacg aaaaccgacg ccctgcactt tgccacgaat acgcagttgg
     4981 acaccgcaag atgtgttttt tgccattgag ttattcccgc catcatgaat tgcgtaaccc
     5041 gcccagccgg acacgacagc gtcgcatccg gcagtcacag gtcggcgata ccgcccgctc
     5101 cgtattctac gaatatttcc gggaattcct ttgatgccag aacagttctg taagattttt
     5161 agaacatcag cgccgtacgg cggcgttttt ctgcgctcag ttgttcaagt ttattgcgat
     5221 cgacacaaat tagcgcatgg gtcgggcaag ccgccataca cgccgggccg tcttcacgat
     5281 ggttgcacag gtcgcattta ttggcttcgg ctttgtcagc ccgtacattc agccccgcgc
     5341 cgctgttgcg aatcaccgga cgtaccacca cttccatcgc gccatacggg caagccacaa
     5401 cgcaggtttt gcaaccaatg caacgttcct gcatcacatg aacaaaccct ttatcacggc
     5461 tgatagcacc attcgggcag acgttagcgc acggtgcatc ttcacactga cggcaaactg
     5521 tcgccgtgga aatgttcaca cctttaatga catggatacg cggtaaaaat gtttccgggg
     5581 tcagcgatgc acagtcctga ttttcctgat gagaaaccac gcacgccact tcacaggtac
     5641 ggcaaccaat acatttactc gcgtcagcaa tgatgaaacg gttcatcaaa ttctccagca
     5701 atgacagtta atgcgccgat acattcacaa atcatgccag tttttaattt actgttattt
     5761 aaggaaatta atttctgtaa tgcaggaaaa aagatgtcat cgacactagt gacgatgaca
     5821 tgtgatgaca atgtttatcg cgaaagagca atgagtgagt cgcggcggat cagttttccg
     5881 ctgaaggttt tcggcggtgt gtaagcaccc ccatcgagca taaaaatcag ccgtccaata
     5941 atttcctgaa tcatctcagt caccggaatt tttacgctgg agagcgccgg aacgatatag
     6001 ggggcaattg cgatatcatc gaatccgata actgacacct gctctggcac cgctacgccg
     6061 cgctcgtgca acgctttcat cgcacctatc gccatatcgt cgttactggc aactaacgcg
     6121 ctaaatttag ccccacgttc gagcaacgtt tctacccctt cggccccgct ggcgggcgtc
     6181 catttaccgt tagcgataag ttgttcattg agcgcaatac catgctgcgc cagcgcgtct
     6241 ttatacccgg caagacgttc aatgctggtg ggggaatcca tcgagccggt aaggaaagca
     6301 atctcctgat gccgggcgtt tatcaactct gccacggcgt taaagctggt ctgtttatga
     6361 tcgcaccaga cgctatggct gctatttttg cgcaggcggc gattaagcac cattatcggc
     6421 tgactgtgcg cgtcaatgat gtcatcgatc tcatccacgc ttaaaaaacg cgggtaaatc
     6481 ataatggcgt cgcagcgcaa atccagcaga tactgaatcg cctggcgctc ttcttcggcg
     6541 ctgtgtttac catctgccaa tagcaactgt cgccctttct cttccgccat tcgcgcggca
     6601 tgaaacagta attcactaaa ataaatgccg tggtaaagcg tgttggtcac taccagcccc
     6661 agcgtctgag tactcttcgc cgacagattg cgcgccagca agtttgggcg gtaaccgctc
     6721 tcttctaccg cctgaaacac gcgatcttta gtctcctggc tgacgtagcc attacctgaa
     6781 agcacgcggg aaacggtcgc ttttgaaacc ccggcgcgct tcgccacttc cagcatcgtc
     6841 gtcatcattt tcatcccttt acacgcaatc aacgcagtgt actgcaccgt ttgccgattg
     6901 tccttgcgca atcggcggga aaaatattca ggtgaccggt ttcacaaata taaaaaatga
     6961 acaattcact ctcttgctta tttagtgaca actattcatg attttgtgaa accggtttct
     7021 taattccgtt tcagcatcgg catttttccg tcacgtcgac tgataacaac tacatctacc
     7081 ctactgataa caggataaaa tccgatggcc aaaaattatg cggcgctggc acgctcggtg
     7141 atagcggcac tgggcggcgt tgataacatc tcggcggtca cgcactgtat gacgcggttg
     7201 cgctttgtta tcaaagatga tgcgcttatc gacagcccga cgttaaaaac catccccggc
     7261 gtgctcggcg tggtacgtag tgacaaccag tgtcaggtga ttatcggcaa taccgtttca
     7321 caagcctttc aggaagtcgt cagcctgctg ccgggagata tgcagcccgc acagcccgtg
     7381 ggtaaaccca aactcacgct acgtcgcatt ggtgcgggga tcctcgatgc gctgatcggc
     7441 accatgtcac cgctgatccc ggcgattatc ggcggatcga tggtcaaact gctggcgatg
     7501 atcctcgaga tgagcggcgt gctgacaaaa ggatcgccga ccttaaccat tctgaatgtg
     7561 attggtgacg gtgctttctt cttcctgccg ctgatggtcg cggcatctgc cgccatcaaa
     7621 tttaaaacca atatgtcgct ggcgattgcc atcgcgggtg tgctggtaca tccgagtttt
     7681 attgaactga tggcgaaagc ggcccagggt gaacatgtcg aatttgccct gattccggtc
     7741 accgcggtga aatacaccta cacggtgatc ccggcgctgg tgatgacctg gtgcctgtca
     7801 tatatcgaac gctgggtgga cagcattaca ccggcggtga ccaaaaactt cctcaaacca
     7861 atgctgattg tgttgattgc cgcaccgctg gcaatcctgc tgattggccc gattggtatc
     7921 tggatcggta gcgccatttc ggcgctggtt tacaccattc atggttatct gggctggctt
     7981 tcagtcgcca ttatgggcgc gctgtggcct ctgctggtaa tgaccgggat gcaccgcgtc
     8041 tttacgccaa ccatcattca gaccattgcc gaaaccggca aagaagggat ggtcatgccg
     8101 tcagagatcg gcgctaacct gtcgctgggc ggttcatcac tggcggtggc gtggaaaacg
     8161 aaaaacccgg aactgcgcca gacggcgctg gctgcggcgg catcagccat tatggcgggg
     8221 atttccgaac cggcgttata cggcgtggcg atccgcctga aacgtccgct tatcgccagt
     8281 cttatcagcg gttttatttg cggcgcggtt gccggtatgg cggggcttgc cagccactca
     8341 atggcagcgc cggggctatt taccagcgtg cagttcttcg atccggcgaa tccaatgagc
     8401 atcgtctggg tgttcgcggt catggcgctg gcggtggtgc tgtcgtttat cctcacacta
     8461 ttgctcggct ttgaggatat tcctgttgag gaagcggctg ccgaggcgcg aaagcatcag
     8521 agcgtacaac cgaccgtcgc caaagaagta agtcttaatt gaggatgaaa atgtcagtat
     8581 ttccagaagg ttttttatgg ggcggcgcgc ttgccgccaa ccagtctgaa ggtgcgttcc
     8641 gtgaaggtgg caaaggactg acaacggtcg atatgatccc acatggcgag catcgaatgg
     8701 cggtgaaact ggggctggaa aaacgttttc agttgcgcga tgacgagttt tatcccagcc
     8761 atgaggcgac ggatttttat catcgttata aagaagatat cgccctgatg gcagagatgg
     8821 gattcaaggt tttccgtacc tcaattgcct ggagccgcct cttcccgcag ggcgatgaac
     8881 tgacgcccaa ccagcagggc attgcttttt accgttctgt ctttgaagag tgcaaaaagt
     8941 acggcatcga accgctggtc acgttgtgcc acttcgatgt gccaatgcat ctggtcaccg
     9001 aatatggctc ctggcgtaac cgcaagctgg tggagttttt cagccgctac gcccggacct
     9061 gctttgaagc atttgatggt ctggtgaaat actggcttac cttcaatgaa atcaacatta
     9121 tgttgcatag cccgttctcc ggcgcgggtc tggtgtttga agaaggtgaa aatcaggatc
     9181 aggtgaaata tcaggccgcg catcaccagc tggttgccag tgcgctagcc accaaaatcg
     9241 cccatgaggt taacccgcaa aatcaggtgg gctgtatgct ggcgggcggt aacttctacc
     9301 cttacagttg caagccggaa gatgtctggg cggcgctgga gaaagaccgg gaaaacctgt
     9361 tttttatcga tgtgcaggcg cggggcgcgt atccggttta ctctgcccgc gtattccgcg
     9421 aaaaaggggt aaccatcgac aaagcaccgg gcgatgatga aatcctgaaa aacaccgtcg
     9481 attttgtctc tttcagctat tacgcctcgc gatgcgcctc ggcggagatg aacgccaaca
     9541 acagcagtgc ggcgaacgtg gtgaaatcgc tgcgtaaccc atatttacag gtaagcgact
     9601 ggggctgggg aattgatcca ctcggtctgc gtatcaccat gaacatgatg tacgaccgct
     9661 atcagaagcc gctgtttctg gtggaaaacg gcctcggcgc aaaagatgaa cttgctgcca
     9721 atggcgagat taacgatgac tatcgcatca actatttacg cgaacatatc cgcgcaatgg
     9781 gcgaagcgat tgcagatggc attccgctga tgggctacac cacctggggc tgtattgatt
     9841 tagtttccgc ctctacgggt gaaatgagca aacgctacgg ttttgtctac gtagaccgtg
     9901 acgacgcagg caacggcacg ctgacgcgca cgcgtaagaa atcgttctgg tggtataaaa
     9961 aagtgattgc cagtaatggt gaggatttag agtaagcaac cgtgccggat gcggcgtgaa
    10021 cgccttatcc ggcctacatg tcgcacgaac caggtaggcc cgataagcgc agcgcatcgg
    10081 gcaatctggc gtttgctatc agttctgatt ccctcatcaa gaacatccct gtcctgattc
    10141 cttactgaaa aactactctt cttctaccgc taactgcgcg aaaccgccat tcccttccca
    10201 gccttccaga cgttgataaa cggtttcaac cgcatcttta atcggctggg tcatcgggta
    10261 gtaaaagccg acgatatccg gctggatgcc gagaaaaatc acttcgccaa tatcttcttt
    10321 caactggtcg ataagatagt tcagaggcat gttatgggta gtcatcataa acatctcggc
    10381 gatatcatcc gggtcgatga tgcggatttc gccggggttt agccccatat cagtagcgtc
    10441 aacaatcagt aaccgcgtag ggcgcagttc acggatggcg acgatgtcgt tttccggtgc
    10501 actaccgcca tcaatcacca cccagttacc tttcggcgcg gcggcgcact tttccgccag
    10561 cagcggacct gcgccatcat cgcccatcat gctattgcca acacagagta aaacgtcagt
    10621 cacgcagtct cctcaccatc aaatagatgg cgttttcctg atgaatatca tgcagcatac
    10681 ttaacagcat tgtactccat tcctgctgct gcgaagtttg cgctgcccgg gctttatcga
    10741 atgcattggc cagcatcggc acatggttaa tgtcgatgac gatctcgcca tatttcggta
    10801 cgccttccat tttgcggcgg gcttcgctgc ccgcctccag agtggcaatc catgccagat
    10861 attcatccca tgggcaggtg agcgccgctt ccaggcaatc gataacccca aggtggtgac
    10921 caatcgccag gctgtaatag accacctgct gcgcctcggc gggcgtggca tcgttctcat
    10981 caataaattt acggctcagt tgactgaaca ccaccttttc actcatcgga tacgcgcctc
    11041 ttcaacaaca tgattcagat ggctgacaat ctcgttcaga cgcggatcgt tttccgcttc
    11101 cagccagcgt gcaacctgtt cttcgccctg ccctaactgt gtaaggtaat catcggcaat
    11161 ctgacgaccg taacgataac ccgccaggcg acgtgcttcg cgatccactt tcacgcgcag
    11221 cggctgcacc atatcgccat gcagaatctc agccggttgt tcatcctgct cacccgggcc
    11281 acgggcgtga attttctgct ccagcaggcc gagtgccatt gcaaagccgt acagcgtggc
    11341 ggcaggcgtt ggcgggcaac ccggaatgta gacatctacc gggacgattt tatccgtacc
    11401 gccccacacg cagtagagat cgtggaaaat accaccgctg ttaccgcagg caccgtagga
    11461 gatacaaatt ttcgggtccg gcgcggactg ccacgcacgc agcgcagggg atcgcattgc
    11521 acgggtgacc gcgccggtaa acagtaaaat atccgcatga cgcggtgaag gaacgacttt
    11581 aatgccgaag cgttctgcat caaacagcgg cgataaagtg gcgaaaattt cgatttcgca
    11641 gccgttgcag ccgccgcagt ccacgcggta aacataggca gaacgtttga tttttttcag
    11701 taacgacgcc ttcatgctgg cgatggattc atccaccgtc atcgggaccg gaatgccgtt
    11761 ggcgtcacgg gggcctaata aattgctcat cagatggcct ctttcatatg gcgagtcagt
    11821 tcaatacggt cggacggcac caggcatttc tggcgcttac attccgggca agtctcaaag
    11881 ctttcgcggt ggttttccgc gcggctgtcg ccgttgtgct taagcagcgc aatggcgtag
    11941 tcgatctctt tctggacggc gaaaggacga ttgcagacgc gacagttgca cagcgcaaag
    12001 cgggactgtt gcaggaaatc ttcttttttc cacaccgcca gttcgtactc ttgcgacagt
    12061 ttgatcgccg ccgtcgggca aacttcttcg cagcgtccac agaagatgca gcgcccgaga
    12121 ttaaactccc acgcaagctc tcctgtggcg aggtcagttt caaccgttaa ggcgtttgac
    12181 gggcaggcat tgacgcaggc cgcgcagccg atgcactgct gcgggttctg ctctggctta
    12241 ccacggaagt ttttatcaac cgcaatcggc tccagcggat aagacgaggt cgccgtgccg
    12301 gttttgatga cttttttgat aaaggtaaac atggcgattc cttatttcag cggcgagttt
    12361 ttacgctcaa tgctgtaacg ctcgagttct ttgtacggca ccactttgct cttcttctta
    12421 cgcacatcga ctacggtcat gcggtcggta caggagtagc aagggtccag gctaccgata
    12481 atcagcggcg catcggaaac ggtgttgccg cgcagcatgt agcgcagggt cggccagttc
    12541 gcgtaggtcg cggcacggca gcgccagcgg tacagcttct ggttgtcgcc ggtcatgctc
    12601 cagtggatat catcgccgcg cggcgcttcg gcaaagccca gcgcaaagcg gtgcggaatg
    12661 taggtaaagc cttccaccat cagtgggcca cccggcaggt tatccagacc gtagtcgatc
    12721 atgttcagcg cggtatagac ttcgttgata cgcactttca gacgggaaat aacgtcgcag
    12781 ccctgctcgc tgtggacttc cattggcagc aggccataac cgacaaacgg gtgatcggca
    12841 cgggtatcgc gggcgtgacc gctggcgcgg accatcgggc cgacgttact gaagtcgcgg
    12901 gcaatttccg ggtccagacg accaatgccg acagtgcgtt gttccatgtt cggtgtgctc
    12961 aacagcacat ccaccagctc ctgcacttca cgacgcatct gttgcgccag ttgacgggtc
    13021 tggatcatgt catctttcag cagatcgcga cgaatcccgc cgatcaggtt caggccgtag
    13081 gttttacgcg cgccggtgag gatctccgcc attttcatag aggtttcacg cacgtggaag
    13141 aactgcataa agccggagtc aaagccggta aagtggcagg cgagaccgag gttgagcaga
    13201 tgcgagtgca ggcgttccac ctccagcaga atggcgcgga tcatctgcgc acgttctggc
    13261 acctgaatac ccatcgcgtt ttctaccgac gtggtgtagg cggtgctgtg ggcaaagccg
    13321 cagatcccgc acacacggtc agagaggaag gtcacttcgt tgtaacccat acgggtttcc
    13381 gccagttttt ccataccgcg atgaacgtag aacaggcggt agtcggcgtc gataatgttt
    13441 tcgccatcga cgaacagacg gaagtggccc ggttcgtcgg aagtgacgtg cagcggacca
    13501 atcggcacga cgttgttttt cttgtcgccc agttcgttga tgaactcgta ggtttcagca
    13561 tcggtggtcg gcgccggacg ctgacgataa tccatgctgt ctttacgcag cggataaagt
    13621 tcatccggcc agtcatccgg cagcaccaga cggcgttcat ccggcagacc aaccggaatc
    13681 aaaccgtaca tatcgcgcac ttcgcgctcg ccccacaccg ccgccggaac gcgcggtgtc
    13741 acggacggat attccggttt gttggcgtca acttcgacgc gaaccgtaat ccaacactta
    13801 gtgcccttct ccatcgacag cacgtagtaa acggcgtaat gaccattcag tttgcgttcg
    13861 tcgttaccaa acagcaccga cagccagcca ccctgtttgt agtaaagaaa ctccaccact
    13921 tccggcaggt agttcacctt tacggtgaca gtcagctgat ctttggtctg ccaggcgtgg
    13981 tccagcacga cgcccggaaa tgcctcattc agcgcggcga gataatgttg acctaatttt
    14041 tcttcagaca tgctcaaact ctctttaatc acgccgccag caaggagacg aacgctaaaa
    14101 atgcaaagcc aaacccagcc caggtaatgc gcggagtaat atcaagacgc agacgggcca
    14161 tgctgttttc gaacagcgcg ataaccagga cgccgaccac cagttttacg atggcaatca
    14221 ccagcgccag cagcagtcca ccggcggtga aggtttccat ttgtccccac ggaataaaca
    14281 ccccgacgaa catctgcagc accaccagct gtttcaggct gataccccat ttcatgacgc
    14341 caaagccgct gccgctatac tcagagagcg gaccttcctg caactcctgc tcggcttccg
    14401 ccaggtcgaa cggcagtttg cccatttcga taaaagtggc gaacgcacag gcacaaagcg
    14461 ccagtaccag cgggatgctc tggctcagcg gccagtgata aacggtgtcg gtgatgttgc
    14521 tgatgttggt ggaaccggca acctgtgcgg caacccacag accaagcagc agcatcggtt
    14581 cgaccagcac gccaagcatc gcttcacggc tcgcgccgat agcggtaaac gggctaccgg
    14641 tatccagacc agaaatggca aagaagaaac gcgcgatggc aaagagatac agtaaggtga
    14701 ttaaatcacc cagttgcggc agcggagaac cgacggtcac caccggcagc gcagtagcga
    14761 tagtcagcat gacgcccacc atcacatacg gcgtcaggcg gaacacccag ccggaggcat
    14821 ccgggccaac gctctggcgg cccagcagtt tgaaaatgtc acgatactcc tgcaacacac
    14881 ccggcccacg acggttatgc agacgggcgc gcgccacgcg ggtaataccg gagagcagcg
    14941 gcgcaacggc aaataacacc agcgcctgaa ttaacggata taaaacactc attctcaggc
    15001 tcctcgtgaa acaataatca ccaccagtac cgccagttca accagcgcca tccggcggaa
    15061 cagcaacgca ctaccttcgc actgccagcc cggcaccagc gacaccggat tcagccattt
    15121 gcgcagtttc agcactggcg caaacgcctg tttcaccggc atggcaaaac cgtgagcggt
    15181 aatcaccatt gatttttcgt ggtcgtagcc gcacacccag gccgcgccgc gggaacgcga
    15241 tggcaaacga tcgcctttgc aaatcgccat aatgatgaat ggcagcagcg ggcaggcaat
    15301 cagcagcaac gtgatcatcg gttgagaaac ggtggtgtta gcaggctcca gcggcagagg
    15361 tacagcagca gacagcatcg gcagcagcca cggcgcagca acaccgccaa ttacgcagca
    15421 aatcgccagt gccactacgc ttacgctcat caggagcggt gcacaggtgg cgttttcggc
    15481 ttctttggta cgcggcgcgc cgaggaacgt gacgccatag actttcgcca tacacatcac
    15541 cgccagcgca ccggtaattg ccagacccac ggcgagcagc ggccccagca gacgggcaac
    15601 aaacgcgcca ctattgctca gtttgaagaa ggattgatag ataacccatt ccccggcaaa
    15661 accattcagc ggcggcagcg cagccattgc catcagcccg actaacatgg cgatggagat
    15721 aaccggcatt ttcttgccaa taccaccgag tttttcgata tcgcgatgac cggtacggaa
    15781 ccagacgctc cccgctccga ggaacagtac gcttttgaac aggctatggt taagcagatg
    15841 gtacagacca ccgaccaggc caagagcaat cagtgccggt tgttcgagcg cgatacccgt
    15901 tacgccagcg cccagcccca gcaggataat gccgatattt tccagggtgt ggtaagccag
    15961 caggcgctgg atgttgtgtt ccatcaacgc atacagacca ccgacgaacg cggtgatcat
    16021 gccgagcacc agcagcgcga tcccccacca cagcggtgca ttaccgccca gcagtgacag
    16081 ggttaaaatg cccagcaggc caattttcat gactaccgta gaaaacaacg cggcagctgg
    16141 cgcagaggcg ttagcatgtg cctgcggcac ccagccgtgc agcggaataa tcccggccag
    16201 caggccaaag ccaatcacac cgagcagcca gatatcggaa ccgagcggca gctgttgcat
    16261 acgcatatcc agcaggcgca gatccagcgt gccataacgc tgccacagca gccagcaagc
    16321 aatcgccagc agcagagtgc caagacgccc cagcgcaaac cacagtttgc cctctttgct
    16381 gttgctggtg aggaacaccg cgcacagggc catgatttcg gccattacca cgaacatgcc
    16441 gaggttgctg gcaatgacgg cgcagacggc ggcagccatc aacatattga tctgcaagcc
    16501 gttgcacttc acctgcgcgt ggcgatgcca gtcaatgttg tagaggctga caaacagacc
    16561 gcacagaccg agcgtaatca gccaaatcgc gttaagcgga gaaatttgca catcgtagct
    16621 taccagcgac agcgcaccgc tcacgccaac cgcgccagtc agtacagtga agcccgcggc
    16681 tgccgtatac agactaccaa ccgcgccgcc aattccagct atccagccac ttaacgcttt
    16741 ttgaaaagaa aagagaaatg ccagaacagc ggcggcgaca aaccacgcca cgccgctatt
    16801 gatcagggaa attgcgctca tttagcctct ccactttgag cctgctgaaa caaggtgaga
    16861 tcgccaaagt ccgtgttaaa agtcagctca cgcttacgtt tgctgacgcg ggcgatatca
    16921 gtgttatcca ccagatgcag ggctttagtc gggcacatcc gcacgcaggc cggaccttgt
    16981 tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc gaatacctgg cacccagtca
    17041 agcaatgtgc tgacacgcgc cggagcaggc ggtgccggtg gcgctttcgg ggtattggcg
    17101 tttgccggaa tatccagcgg acggctgccg gaaaattcaa ttgcgccaaa cgggcaggcg
    17161 atgccgcaca gcttgcagct tacgcacagg ctttcattca actgcacggc cccatcaacg
    17221 cgggtgatgg cgttaaccgg gcagaccacc gcgcagggtg catcttcaca gtggtgacag
    17281 agctgcggcg cagattcttt ttcattcagc atcactctca ggcgcggcat tgattgcagg
    17341 ccgtgctggc gatgcgtctc tgaacaggcg gcctcacaag tgtggcagcc gatacagagc
    17401 gtggagtcag caattacaaa acgattcacc aggcattcct caggtgattg tcatttttga
    17461 cgaaaacatg ccgatgaaat gtcattttcg acactcatcg acacgcccat ccccaaacag
    17521 gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct gccgggcttt gtccctttac
    17581 cagttggctt aaattcaccg gcacattgtt ttcaatagcg gtgtataagc tgtcgtaaac
    17641 gccagcgatt ttttcgagat agtgttccag aacctgttcg cgatcgcgga tgttgacctt
    17701 gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca atccgctgtt tgtcttcgcc
    17761 atcttttgtt tcgacgtaat actcgatggt gtggctgtta aagccatccg ccagggtgac
    17821 gtaaatgcga aaatgttcaa aaaggacgaa acagagttct ttgtccggcg cgcagctcat
    17881 ggcgtgatgc aggcgcgctt tcgataacgt gatcccctga accagcgaat tgcagtagat
    17941 gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg taatcggctt tctcgcttat
    18001 ttcccaaata gtcattgtca ggttacccgt ttaacagaga tgccagcttt aagcattttt
    18061 tgtgccaact tttaatttat tgttattaaa gagattttta agctaaagat gaatttcgtc
    18121 gccgtgtcga cgtgtcattt cgacatcatc gacattattc accgcaggga taatcaacac
    18181 tggcacaatt attgcttgta gctggcaata gttaatggga ggcgatatgc acgaaataac
    18241 cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg gcgcaaaacg
    18301 cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca gctctcttgc
    18361 cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac tgcacctcga
    18421 agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc tactgaccca
    18481 gcgcgtccgc cgctgtccac agtgtcatgg tgacatgctg cagattgtgg cagacgacgg
    18541 tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa catgcggttg
    18601 cggtgaaggc aacctgtata tagagggtga tgaacataac cctcattccg cgtttcgtag
    18661 cgcgccattt gccccggcgg cacgcccgaa gatgaaaatc accggcatta aagcgcctga
    18721 atttaccccc agccagactg aagaaggcga cctgcattac ggtcatggcg aagcgggcac
    18781 tcacgcaccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg tgctggacaa
    18841 aaataaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc aactggtgct
    18901 caacctggtt tccagccctg gttccggtaa aaccaccctg ctgacggaaa ccctaatgcg
    18961 cctgaaagac agcgttccgt gcgcagttat tgaaggcgac cagcaaaccg tgaacgatgc
    19021 cgcacgcatt cgcgctaccg gcacaccagc gattcaggtg aataccggta aaggctgcca
    19081 tcttgacgca cagatgattg ccgacgccgc accgcgcctg ccactggacg ataacggtat
    19141 tctgtttatc gaaaacgttg gcaacctcgt atgcccggcc agcttcgatc tcggtgaaaa
    19201 acacaaagtg gcggtgcttt ccgttaccga aggtgaagac aaaccgctga aatatccgca
    19261 tatgtttgcc gccgcctcgc tgatgctgct caacaaagtt gacctgctgc cgtatctcaa
    19321 ctttgacgtt gagaagtgca tcgcctgcgc ccgcgaagtc aatccagaaa ttgaaatcat
    19381 ccttatttcc gccaccagcg gcgaagggat ggaccagtgg ctgaactggc tggagacaca
    19441 gcgatgtgca taggcgttcc cggccagatc cgcaccattg acggcaacca ggcgaaagtc
    19501 gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg cgatgaaaac
    19561 ggtcagccgc gcgtgggcca gtgggtactg gtacacgttg gctttgccat gagcgtaatt
    19621 aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga cgttgagccg
    19681 gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat gaatatcgcg
    19741 cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca catctctctt
    19801 acacagccga acgccctctg cggattatgg aagtgtgcgg tggtcatacc cacgccattt
    19861 ttaaattcgg cctcgaccag ttactgcctg aaaacgttga gtttatccac ggtccgggtt
    19921 gcccggtgtg cgtactgccg atgggtagaa tcgacacctg cgtggagatt gccagccatc
    19981 cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt gccggggaaa cagggatcgc
    20041 tgttgcaggc aaaagcacgc ggtgccgatg ttcgcatcgt ttactcgcca atggatgcgt
    20101 tgaaactggc gcaggagaat ccaacccgca aagtggtgtt cttcggctta ggttttgaaa
    20161 ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa agcccgcgat gtgcagaatt
    20221 tttacttctt ctgccagcat attacgctta tcccgacgct gcgcagtttg ctggaacagc
    20281 cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt tagtatggtt atcggcactg
    20341 atgcctataa ttttatcgcc agcgattttc atcgtccgct ggtggtcgct ggtttcgaac
    20401 cccttgatct actgcaaggc gtggtgatgc tggtggagca gaaaatagcg gcccacagca
    20461 aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctgctg gcgcaacagg
    20521 caattgctga tgtgttctgt gtcaacggcg acagcgaatg gcgcggctta ggcgtgattg
    20581 aatcttctgg cgtgcacctg acgccggatt atcaacgatt cgatgccgaa gcacatttcc
    20641 gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcacg ttgtggtgaa gtattaacgg
    20701 gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct caaaccgcat
    20761 ttggtgcact gatggtctcc tccgaaggag cgtgcgccgc gtggtatcag tatcgtcagc
    20821 aggagaatga agcgtgaata atatccaact cgcccacggc agcggcggcc aggcgatgca
    20881 gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg cagagcagga
    20941 agaccaggca cgtcttgatc tggcgcagct ggtagcagaa ggcgatcgtc tggcgttctc
    21001 caccgacagt tacgtcattg acccgctgtt ctttcctggc ggtaatatcg gcaagctggc
    21061 gatttgcggc accgcgaatg acgttgcggt cagtggcgct attccgcgct atctctcctg
    21121 tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag tgaccagcat
    21181 ggcagaaacc gcccgcacgg caggcattgc catcgttact ggcgatacta aagtggtgca
    21241 gcgcggcgcg gtagataaac tgtttatcaa caccgctggc atgggcgcaa ttccggcgaa
    21301 tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttgctggtga gcggtacact
    21361 cggcgaccac ggggcgacta tccttaacct gcgtgagcag ctggggctgg atggcgaact
    21421 ggtcagcgac tgcgcggtgc tgacgccgct tattcagacg ctgcgtgaca ttcccggcgt
    21481 gaaagcgctg cgtgatgcca cccgtggtgg tgtaaacgcg gtggttcatg agttcgcggc
    21541 agcctgcggt tgtggtattg aactttcaga agcggcactg cctgttaaac ctgccgtgcg
    21601 tggcgtttgc gaattgctgg gactggacgc cctgaacttt gccaacgaag gcaaactagt
    21661 aatagctgtt gaacgcaacg cggcagagca agtgctggca gcgttacatt cccatccact
    21721 ggggaaagac gcggcgctga ttggtgaagt ggtggaacgt aaaggtgttc gtcttgccgg
    21781 tctgtatggc gtgaaacgaa ccctcgattt accacacgcc gaaccgcttc cgcgtatatg
    21841 ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc tttgcggtgc
    21901 tttcctggaa gaacaaaatg tcatatacac cgatgagtga tctcggacaa caagggttgt
    21961 tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt gaggctcttt
    22021 cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg tggcaagcgc
    22081 agactcaacg tgcgtcttat tacgcgtcgc gtgaaaaaga cacccccatt aaatatgaag
    22141 acgaaactgt tctggcacac ggtccggtac gcagcatttt gtcgcgccct gatacgctgc
    22201 attgcagtta cgaagaattt tgtgaaacct ggccgcagct ggccgcaggt gggctatacc
    22261 caaaatttgg tcactattgc ctgatgccac tggcggcgga agggcatatt tttggtggct
    22321 gtgaatttat tcgttatgac gatcgcccct ggagcgaaaa agagttcaat cgtctgcaaa
    22381 catttacgca gatcgtttct gtcgtcaccg aacaaatcca gagccgcgtc gttaacaatg
    22441 tcgactatga gttgttatgc cgggaacgcg ataacttccg catcctggtc gccatcacca
    22501 acgcggtgct ttcccgcctg gatatggacg aactggtcag cgaagtcgcc aaagaaatcc
    22561 attactattt cgacattgac gatatcagta tcgtcttacg cagccaccgt aaaaacaaac
    22621 tcaacatcta ctccactcac tatcttgata aacagcatcc cgcccacgaa cagagcgaag
    22681 tcgatgaagc cggaaccctc accgaacgcg tgttcaaaag taaagagatg ctgctgatca
    22741 atctccacga gcgggacgat ttagccccct atgaacgcat gttgttcgac acctggggca
    22801 accagattca aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg ctgggcgtgc
    22861 tgaaactggc gcaatgcgaa gagaaagtgt ttaccactac caatctgaat ttactgcgcc
    22921 agattgccga acgtgtggca atcgctgtcg ataacgccct cgcctatcag gaaatccatc
    22981 gtctgaaaga acggctggtt gatgaaaacc tcgccctgac cgagcagctc aacaatgttg
    23041 atagtgaatt tggcgagatt attggccgca gcgaagccat gtacagcgtg cttaaacaag
    23101 ttgaaatggt ggcgcaaagt gacagtaccg tgctgatcct cggtgaaact ggcacgggta
    23161 aagagctgat tgcccgtgcg atccataatc tcagtgggcg taataatcgc cgcatggtca
    23221 aaatgaactg cgcggcgatg cctgccggat tgctggaaag cgatctgttt ggtcatgagc
    23281 gtggggcttt taccggtgcc agcgcccagc gtatcggtcg ttttgaactg gcggataaaa
    23341 gctccctgtt cctcgacgaa gtgggcgata tgccactgga gttacagccg aagttgctgc
    23401 gtgtattgca ggaacaggag tttgaacgtc tcggcagcaa caaaatcatt cagacggacg
    23461 tgcgtctaat cgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac cgtgagttcc
    23521 gtagcgatct ctattaccgc ctgaacgtat tcccgattca cctgccgcca ctacgcgagc
    23581 gtccggaaga tattccgctg ctggcgaaag cctttacctt caaaattgcc cgtcgtctgg
    23641 ggcgcaatat cgacagcatt cctgccgaga cgctgcgcac cttgagcaac atggagtggc
    23701 cgggtaacgt acgcgaactg gaaaacgtca ttgagcgcgc ggtattgcta acacgcggta
    23761 acgtgctgca gctgtcattg ccagatattg ttttaccgga acctgaaacg ccgcctgccg
    23821 caacggttgt cgcccaggag ggcgaagatg aatatcagtt gattgtgcgc gtgctgaaag
    23881 aaaccaacgg cgtggttgcc gggcctaaag gcgctgcgca acgtctgggg ctgaaacgca
    23941 cgaccctgct gtcacggatg aagcggctgg gaattgataa atcggcattg atttaactgc
    24001 aaattgccgg acagatctgc ctgtccggca tactattcat gaggtttttt cggacgatat
    24061 ttttccggca gttctggcac cggacgcttg tcatcgatga gatgacgcac ggttaagatc
    24121 ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat ctcttcacgc
    24181 tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggtttctc ttcgccgaac
    24241 acacatttat ccagccgctt ttgcgcgtaa acaaacaacg cctcgtaatg ctccggctcc
    24301 gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat cgtcagtttt
    24361 tcacgagaga tacgcttgcc ggacatgctg cctccacctc attaagatgt atttatatta
    24421 catcttaatc ttaaagggca ctatgactct aaagaagaag ggttcgccaa ccgacgcaat
    24481 tttgcgtact tgcttcataa gcatcacgca aaagctgcaa aacagcatct ttcccggaac
    24541 cagcatcaag aactcgccgt tcgcttcttc ccctgaaatg attaactccg gtatcatgtg
    24601 cgccttatgt gattacaacg aaaataaaaa ccatcacacc ccatttaata tcagggaacc
    24661 ggacataacc ccatgagtgc aatagaaaat ttcgacgccc atacgcccat gatgcagcag
    24721 tatctcaagc tgaaagccca gcatcccgag atcctgctgt tttaccggat gggtgatttt
    24781 tatgaactgt tttatgacga cgcaaaacgt gcgtcgcaac tgctggatat ctcactgacc
    24841 aaacgcggcg cttcagcggg agagccgatc ccgatggcgg ggatccccta ccatgcggtg
    24901 gaaaactacc tcgccaaact ggtgaatcag ggcgagtccg ttgccatctg cgaacaaatt
    24961 ggcgatccgg cgaccagcaa aggtccggtt gagcgcaaag ttgtgcgtat cgttacgcca
    25021 ggcaccatca gcgatgaagc cctgttgcag gagcgtcagg acaacctgct ggcggctatc
    25081 tggcaggaca gcaaaggttt cggctacgcg acgctggata tcagttccgg gcgttttcgc
    25141 ctgagcgaac cggctgaccg ggaaacgatg gcggcagaac tgcaacgcac taatcctgcg
    25201 gaactgctgt atgcagaaga ttttgctgaa atgtcgttaa ttgaaggccg tcgcggcctg
    25261 cgccgtcgcc cgctgtggga gtttgaaatc gacaccgcgc gccagcagtt gaatctgcaa
    25321 tttgggaccc gcgatctggt cggttttggc gtcgagaacg cgccgcgcgg actttgtgct
    25381 gccggttgtc tgttgcagta tgcgaaagat acccaacgta cgactctgcc gcatattcgt
    25441 tccatcacca tggaacgtga gcaggacagc atcattatgg atgccgcgac acgtcgtaac
    25501 ctggaaatta ctcagaacct ggcgggcggt gcggaaaata cgctggcttc tgtgctcgac
    25561 tgcaccgtca cgccgatggg tagtcgtatg ctgaaacgct ggctgcatat gccagtgcgc
    25621 gatacccgcg tgttgcttga gcgccagcaa actattggcg cattgcagga tttcaccgcc
    25681 gagttgcagc cggtactgcg tcaggtcggc gacctggaac gtattctggc acgtctggct
    25741 ttacgaactg ctcgcccacg cgatctggcc cgtatgcgcc acgctttcca gcaactgccg
    25801 gagctgcgtg cgcagttaga aactgtcgat agtgcaccgg tacaggcgct acgcgagaag
    25861 atgggcgagt ttgccgagct gcgcgatctg ctggagcgag caatcatcga cacaccgccg
    25921 gtgctggtac gcgacggtgg tgttatcgca tcgggctata acgaagagct ggatgagtgg
    25981 cgcgcgctgg ctgacggcgc gaccgattat ctggagcgtc tggaagtccg cgagcgtgaa
    26041 cgtaccggcc tggacacgct gaaagttggc tttaatgcgg tgcacggcta ctacattcaa
    26101 atcagccgtg ggcaaagcca tctggcaccc atcaactaca tgcgtcgcca gacgctgaaa
    26161 aacgccgagc gctacatcat tccagagcta aaagagtacg aagataaagt tctcacctca
    26221 aaaggcaaag cactggcact ggaaaaacag ctttatgaag agttgttcga cctgctgttg
    26281 ccgcatctgg aagcgttgca acagagcgcg agcgcgctgg cggaactcga cgtgctggtg
    26341 aacctggcgg aacgcgccta taccctgaac tacacctgcc cgaccttcat tgataaaccg
    26401 ggcattcgca ttaccgaagg tcgccatccg gtagttgaac aagtactgaa tgagccattt
    26461 atcgccaacc cgctgaatct gtcgccgcag cgccgcatgt tgatcatcac cggtccgaac
    26521 atgggcggta aaagtaccta tatgcgccag accgcactga ttgcgctgat ggcctacatc
    26581 ggcagctatg taccggcaca aaaagtcgag attggaccta tcgatcgcat ctttacccgc
    26641 gtaggcgcgg cagatgacct ggcgtccggg cgctcaacct ttatggtgga gatgaccgaa
    26701 accgccaata ttttacataa cgccaccgag tacagtctgg tattgatgga tgagatcggg
    26761 cgtggaacgt ccacttacga tggtctgtcg ctggcgtggg cgtgcgcaga aaatctggcg
    26821 aataagatta aggcattgac gctgtttgct acccactatt tcgagctgac ccagttaccg
    26881 gagaaaatgg aaggcgtcgc taacgtgcat ctcgatgcac tggagcacgg cgacaccatt
    26941 gcctttatgc acagcgtgca ggatggcgcg gcgagcaaaa gctacggcct ggcggttgca
    27001 gctctggcag gcgtgccaaa agaggttatt aagcgcgcac ggcaaaagct gcgtgagctg
    27061 gaaagcattt cgccgaacgc cgccgctacg caagtggatg gtacgcaaat gtctttgctg
    27121 tcagtaccag aagaaacttc gcctgcggtc gaagctctgg aaaatcttga tccggattca
    27181 ctcaccccgc gtcaggcgct ggagtggatt tatcgcttga agagcctggt gtaataacaa
    27241 ttcccgatag tcttttgcta tcgggaatat taacgacaac tgacgaataa aataaaaaca
    27301 ccctgtataa taggaaagct tattttacag ggtaaaacca tgccatctac acgctatcaa
    27361 aaaatcaatg cccatcacta tcgccatata tgggtcgttg gtgatattca tggtgaatat
    27421 cagttattac aatcccgctt acatcaactc tcttttttcc ccgaaatcga cttacttatt
    27481 tctgtcggcg ataatattga tcgtggaccg gagagtcttg acgtcctgcg cctgctaaac
    27541 caaccctggt ttacgtcggt taaaggcaac cacgaagcga tggcgcttga ggcattcgaa
    27601 actggcgatg gcaatatgtg gcttgccagc ggtggtgact ggtttttcga tttaaatgat
    27661 tcagagcaac aagaggcaat agatctgttg ctgaaattcc atcaccttcc acatattatt
    27721 gaaatcacta acgacaacat aaaatatgcc atcgcacatg cagattatcc ggggagtgaa
    27781 tatctctttg gtaaagaaat agcggagagc gaattactct ggcctgttga tcgtgtgcag
    27841 aaatcgctta atggcgagtt acaacaaata aacggcgctg attattttat atttggacat
    27901 atgatgtttg ataacattca gacgttcgct aaccagattt atattgatac cggatcgccg
    27961 aacagcgggc ggctgtcatt ttataaaata aagtagtctc atgcttcttc tgtgaagcat
    28021 gagtaacccg gtgttattgc aggccattat tcatttttcg ctaccagcaa agagagatcc
    28081 tgcttcacca gcgcgcgact ggcactctcc ggcaaaccgt cgtctgtaat aatctgatca
    28141 aactcgctta atggtaacgc cagccatgtc gccacctgac catatttcgt cgcatcacag
    28201 accaaaactc gctggcggct ggcactggca atcgcccgtt tcaccgtgac tttatcttcc
    28261 gctggcgtag aaatcccccg cacactccat gacgatgcag aaataaaagc ctgatcaatc
    28321 atcaggctgc gcagcatggt cgcagcggct tccccgacac aggaacggtt ttcccgacac
    28381 actgcaccgc cagtgtgaat aattgtgcaa ttactgttgt cgagcaagta gtccgcaata
    28441 acgaaatcgt ttgtgaccac agtcagtgac tccatgtgaa tcagatgctg tgctatcgct
    28501 aacgtggtcg ttcccgcatc cagatagata caacttcccg gctgaacaag acttgccgcc
    28561 agcttgccaa tagccgcttt ttgcgtcatt gccagcgcag tttttacctg atgagaaggt
    28621 tcatgcgcca cgcgtcccgg agactggacg cctccggaca ccagcacaac ggctccctgc
    28681 tgctccagtt tttgtaaatc ccgacgaatg gtcatatgtg acacattcat tctgtccgtt
    28741 agttcagcaa tactgacaat gcctttttca gctaccatct caaggatgat ttggcgacgc
    28801 tctacgggta tcaacttttg ctccttcctt tgtcctgctg acattctacg ctatttgcct
    28861 gcgaaacgtg cgcggcgcaa ctaacgctta gttcacataa aataacacat aatgttaatt
    28921 tatgtgaatc agatcaccat atcgttatct tccagcgctt atattcacaa tatcaaacaa
    28981 aatatcactt aaattaacaa ggagagcaga tgaaaacggg atctgagttt catgtcggta
    29041 tcgttggctt agggtcaatg ggaatgggag cagcactgtc atgtgtccgc gcaggtcttt
    29101 ctacctgggg cgcagacctg aacagcaatg cctgcgctac gttgaaagag gcaggtgctt
    29161 gcggggtttc tgataacgcc gcgacgtttg ccgaaaaact ggacgcactg ctggtgctgg
    29221 tggtcaatgc ggcccaggtt aaacaggtgc tgtttggtga aacaggcgtt gcacaacatc
    29281 tgaaacccgg tacggcagta atggtttctt ccactatcgc tagtgctgat gcgcaagaaa
    29341 ttgctaccgc tctggctgga ttcgatctgg aaatgctgga tgcgccagtt tctggtggtg
    29401 cagtaaaagc cgctaacggt gaaatgactg tcatggcctc cggtagcgat attgcctttg
    29461 aacgactggc acccgtgctg gaagccgttg ccggaaaagt ttatcgcata ggtgcagaac
    29521 cgggactagg ttcgaccgta aaaattattc accagttgtt agcgggcgta catattgctg
    29581 ccggagccga agcgatggca cttgcagccc gtgcggggat cccgctggat gtgatgtatg
    29641 acgtcgtgac caatgccgcc ggaaattcct ggatgttcga aaaccggatg cgtcatgtgg
    29701 tggatggcga ttacaccccg cattcagccg tcgatatttt tgttaaggat cttggtctgg
    29761 ttgccgatac agccaaagcc ctgcacttcc cgctgccatt ggcctcaaca gcattgaata
    29821 tgttcaccag cgccagtaac gcgggttacg ggaaagaaga cgatagcgca gttatcaaga
    29881 ttttctctgg catcactcta ccgggagcga aatcatgatc aagattggcg ttatcgccga
    29941 tgattttacc ggcgcgacgg atatcgccag ttttctggtg gaaaacggtc taccaacggt
    30001 acaaattaac ggtgttccaa caggtaaaat gccggaagca atcgacgcac tggtgatcag
    30061 cctgaaaacg cgctcctgtc cagtggttga agccacacag caatcgctgg cggctctgag
    30121 ctggttgcaa cagcaaggtt gcaaacagat ctatttcaaa tactgctcta ctttcgacag
    30181 tacggcgaaa ggtaatattg gcccggttac cgatgcctta atggatgctc tcgacacgcc
    30241 gtttacggtc ttctctccgg ccctgccggt caacggacgt acggtttatc aggggtattt
    30301 gttcgtaatg aatcaactgc tggccgaatc cgggatgcgc catcacccgg taaatcccat
    30361 gaccgacagc tatcttcccc gtctggttga agcgcaatcc acagggcgct gcggcgtcgt
    30421 ttcggcacat gttttcgaac aaggtgtgga tgccgttcgt caagagctgg ctcgcttaca
    30481 gcaagagggc taccgctacg cggtgcttga tgcgctgacc gaacaccatc tggaaattca
    30541 gggagaagcc ttgcgcgatg ccccactggt aacgggcggt tctggtctgg cgattggcct
    30601 ggcccggcag tgggcgcaag aaaacggtaa ccaggctcgc aaagcagggc gtccgctcgc
    30661 tgggcgcggc gtagtgctct ccggttcatg ctctcaaatg accaaccgcc aggtagcaca
    30721 ttaccgtcaa attgcaccag cccgtgaagt tgatgtggca cgctgcctct caattgaaac
    30781 tctggccgct tatgcacacg aactggcaga gtgggttctg ggccaggaaa gtgtacttgc
    30841 tccactggtt tttgccaccg ccagcactga cgcattggca gcaattcaac agcaatacgg
    30901 tgcacaaaaa gccagtcagg cagtagaaac actgttttct caactagcgg cgcggttagc
    30961 agcggaaggc gtgacacgct ttattgtcgc aggcggtgag acctccggcg tagtcacaca
    31021 gagcctggga ataaaagggt ttcatattgg cccaaccatt tcccccggcg tgccgtgggt
    31081 aaacgcactg gataagcctg tctcactcgc ccttaaatct ggcaacttcg gtgatgacgc
    31141 ctttttttca cgagcccaaa gagagttttt atcatgagcg atttcgcaaa agtagagcag
    31201 tctttgcgag aggagatgac gcggattgcc agttcattct ttcagcgcgg ctatgcaacc
    31261 ggttcggctg gcaatctgtc gctgctttta cctgacggga atttactggc gacaccgaca
    31321 ggttcatgcc tgggcaatct cgatccgcag cggctttcca aagtcgccgc ggatggcgaa
    31381 tggttaagtg gtgacaaacc ctcgaaagag gtgctctttc atctggcgct gtatcgcaac
    31441 aatccgcgct gtaaagcggt ggtgcatttg cacagcacat ggtcgacggc gctttcctgc
    31501 ctgcaagggc tggacagcag caacgttatt cgtccgttca caccatacgt ggtgatgcgg
    31561 atgggaaatg tcccgctggt gccttattac cgaccgggcg ataaacgcat cgcacaggat
    31621 ctggcggaac tggcagcaga caatcaggct tttttactgg caaatcatgg cccagtggtt
    31681 tgcggtgaaa gcctgcaaga agccgccaac aatatggaag agctggagga aacggcaaag
    31741 ctgattttta ttctcggtga ccgcccgatc cgttatctga ccgcaggtga aattgcggaa
    31801 ttaaggagtt aatgcaatgc ctcgttttgc agctaattta tccatgatgt tcaccgaagt
    31861 gccttttatt gaacgcttcg ccgcagcgcg aaaagccggg ttcgatgctg tggaatttct
    31921 gtttccctat aactactcca ccctgcaaat ccaaaagcaa ctggagcaaa accatctgac
    31981 actggcgctg ttcaataccg ctcccggaga tattaatgcg ggggaatggg gattatccgc
    32041 ccttcccgga cgtgagcacg aagcacacgc agacatcgac ctggcactgg aatatgcatt
    32101 ggcgctcaat tgtgaacaag tccatgtgat ggcaggcgtc gtgcccgctg gcgaagatgc
    32161 tgaacggtac cgggcagtat ttatcgataa tatccgctac gctgccgacc gctttgcgcc
    32221 acacggcaag cgaatattag ttgaagcact cagccccggc gttaagcctc attatctctt
    32281 ctccagccag tatcaggcac tggctattgt tgaagaggtt gcgcgagata acgtgtttat
    32341 tcaactagat acttttcatg cgcaaaaagt agatggcaac ctgacgcatt taatccgcga
    32401 ctacgccggg aaatatgcgc acgtacaaat tgccggacta cctgatcgac atgaaccgga
    32461 cgatggagaa atcaactatc cgtggctgtt ccgcctgttc gatgaggtgg gatatcaggg
    32521 ctggatcggt tgtgaatata aacctcgtgg cctcaccgaa gaagggcttg gctggtttga
    32581 cgcctggcgc taattcgtta aatccctaat tacaacgtac ccatacatcc ccccatctac
    32641 ggatggggta ggttaactat aactttcaga cagggtttcc catgtccaca attacattgt
    32701 tatgcattgc gttagctggc gtaatcatgc tgttgctgct ggtcatcaag gcaaaggtac
    32761 aaccattcgt tgctttgctc ctcgtcagcc tgttagtcgc acttgcggca ggtataccgg
    32821 cgggcgaagt gggtaaagtg atgatcgccg ggatgggcgg tgtgcttggt tccgtcacta
    32881 ttattattgg tctgggcgct atgctggggc gtatgatcga acactctggt ggtgcagagt
    32941 cactggctaa ttatttcagt cgcaagttag gtgacaaacg aactatcgct gcgctgactc
    33001 tggcagcgtt cttcctcggt attcccgtct tctttgatgt cggctttatt attcttgcgc
    33061 caatcattta cggttttgcc aaggttgcca aaatatcgcc actcaaattt ggcctgcctg
    33121 tcgctgggat catgctcact gttcacgtgg cggtaccgcc gcatccaggc cctgtcgccg
    33181 cagcggggtt actccacgca gacatcggct ggctaaccat catcggtatt gcgatttcta
    33241 ttcccgtagg ggttgttggc tactttgcag cgaaaataat caataagcgc caatatgcga
    33301 tgtcagtaga agtactggaa cagatgcaac tggctccggc cagtgaggaa ggcgcgacaa
    33361 aattaagcga taaaataaat ccaccgggcg tcgcgctggt cacctcgcta attgttattc
    33421 ctatcgcgat tatcatggcg ggtacggttt ccgcaacact gatgccgcct tcgcatcccc
    33481 tgcttggtac gctacagctg atcggctcac caatggtagc tctaatgatt gcgctggtgc
    33541 tggcattctg gttattggct ttacgtcgcg gctggagctt acaacacacc agcgacatta
    33601 tgggctcagc gcttcctact gcggcggtag tgattttggt tactggtgct ggaggggtat
    33661 ttggcaaagt gctggtggaa tcgggcgtcg gcaaagccct tgccaacatg ctacaaatga
    33721 ttgacctgcc tctgttacca gccgcattta ttatttcact ggcgctgcgt gcatcgcagg
    33781 ggtcagcaac cgtagcaata ctgacaaccg gcgggttact ctcagaagcg gtgatgggat
    33841 tgaatccgat tcagtgcgta ttggtgacgc tggcagcctg ctttggtggg cttggtgcgt
    33901 cacatattaa tgactcaggg ttctggattg tgaccaaata tctggggttg tcggtagcag
    33961 acggtctgaa aacctggaca gtgttaacga ccattctcgg ttttaccgga ttcttaatta
    34021 cctggtgcgt atgggcggta atttgacctt aataaaaagg tccgatgggc atcggacctt
    34081 ttattgtgca cagaaaaggc cagcctcgct tgagactggc ctttctgaca gatgcttact
    34141 tactcgcgga acagcgcttc gatattcagc ccctgcgttt gcaggatttc gcgcaaacgg
    34201 cgcaggcctt caacctgaat ctggcgaaca cgttcacggg tgaggccaat ttcacgacct
    34261 acatcttcca gtgttgccgc ttcgtacccc agcaaaccga atcgacgtgc cagcacttca
    34321 cgctgtttgg cgttcagctc gaacagccat ttgacgatgc tctgcttcat atcgtcatct
    34381 tgcgtggtat cttccggacc gttctctttt tcatcggcca ggatgtccag caacgctttt
    34441 tcggaatcac cacccagcgg ggtgtctacc gaggtaatgc gctcgttaag acgaagcata
    34501 cggctgacgt catcaactgg cttatccagt tgctctgcga tctcttccgc acttggttca
    34561 tggtccagct tatgggacaa ctcacgtgcg gttcgcaggt aaacgttcag ctcctttacg
    34621 atgtgaatcg gcaaacgaat agtacgggtt tggttcataa tcgcccgttc aatcgtctgg
    34681 cgaatccacc aggttgcgta tgttgagaag cggaaaccac gttccgggtc aaacttctct
    34741 accgcgcgga tcagccccag gttgccctct tcgataaggt ccagcaacgc cagaccacga
    34801 ttgccataac ggcgggcaat ttttaccacc agacgcaagt tactctcgat catccggcgg
    34861 cgagaggcga catctccacg cagtgcgcga cgcgcaaaat aaacttcttc ttcggccgtt
    34921 aacagtggtg aataaccaat ctcaccaagg taaagctgag tcgcgtccaa cacacgctgt
    34981 gtggctccct gcgataacag ttcctcttcg gccaaatcgt tatcactggg ttcctcttct
    35041 actaaggcct tttcgtcaaa aacctcaact ccgttctcat caaattccgc atcttcattt
    35101 aaatcatgaa ctttcagcgt attctgactc ataaggtggc tcctacccgt gatcccttga
    35161 cggaacattc aagcaaaagc ctggttccgc cgatttatcg ctgcggcaaa taacgcagcg
    35221 ggtttacgga tttccccttg taacgaattt caaaatgcaa gcgtgttgaa ctggttccgg
    35281 tgctacccat ggtcgctatt ttttgccccg ccttaacttc ttgttgttcc cggaccagca
    35341 ttgtgtcgtt atgggcgtag gcactcaggt aatcatcatt atgtttgatg ataatcagat
    35401 taccgtagcc gcgcagcgcg ttaccagcat aaacaacgcg gccatctgcg gtcgcgataa
    35461 ttgcctgtcc tttgctgcct gcgatatcaa tccccttgtt gcccccctca gaagcgccaa
    35521 aggtttcgat cactttgccc tcagtcggcc agcgccaggt ggagataggc gtactggttg
    35581 atgtactgct gacagtcggc tcggttgtgc ttgctgttgg taccgttaca ggcgctgtga
    35641 ccgtggtcgc agttggcttg ttgttcggca acattttgtt agcactctgt tcacccgaag
    35701 actcagaata cgtaattgtc ggttgcgacg caacagcaac ggtggaattt tgtgcaggct
    35761 tgatcacaac tccttgctct gctgcgtcgg cctgggtaat ggcatttccg ccagtgattg
    35821 gcgtaccgga agcattaccc acctgcaagg tctgaccaac gttcagcgcg tatggtgcct
    35881 gaatattgtt gcgctgagca aggtcacgga aatcgttgcc agtaatccag gcgatataga
    35941 aaagtgtgtc gccttttttc acggtatagg tactgccgct ataactgcct ttcggaatgt
    36001 tcccatactg acggttatag acgatgcgtc cgttttccat ctgtaccggc tgctgagcta
    36061 ctggctgcac tggctggatt tgcggttgtt gagtagcctg aatttgtggc tgctgcaccg
    36121 gctgaatttg cggttgctgc gctgtagatg tcgtccccat tttcggcggc ggcgtaatca
    36181 acataccaga attggtattt gcaggtgcat tgccattaac ggagctgacc ggtgccggtg
    36241 gatttgaagt gtcagaacag cctgccagcc atagcgaaac cagtgacaaa gccgcaatgc
    36301 ggcgaacggt gaattttggg cttcccgcgc tcatttatcc cccaggaaaa attggttaat
    36361 aaccagtgac ataattaccg tgcaaggcac cctactgaac actggaaaag atgttcacga
    36421 tacgctgacc tgcggcaaaa taaccaggaa aaatccaggt atttcctcac gttttaagcc
    36481 agctcaccct tcactaaagg gacaaagcgc acagcctcca cggtatcgat aataaattcg
    36541 cctccccgac gacgcacccg tttcaaatac tggtgctcct cccctacggg taagacgaga
    36601 atcccgcctt cgtccagctg cgtcattagt gcagttggaa tttccggcgg tgccgccgta
    36661 acaatgatag cgtcaaacgg cgcacgtgcc tgccaacctt gccatccatc gccatgacgg
    36721 gttgaaacat tatgtaaatc aagatttttc agacggcgac gcgcctgcca ctgcaagcct
    36781 ttaatccgtt caaccgagca aacatgctgg acaagatgcg ccaggattgc cgtttgatat
    36841 cccgaaccgg tgccaatttc cagcacccgc gactgcggcg tcagctcgag taattcggtc
    36901 attcgcgcca ccatatatgg ctgcgaaatt gtctgcccct gacctatcgg caaggcgata
    36961 ttgtcccagg ctttttgttc aaacgcttca tcaacgaatt tttcacgcgg cacggcggca
    37021 agtgcattca gcacctgctc atcctgaata ccttgcgcac gtaattgatc cagaagtgct
    37081 tgtacgcgtc tgcttaccat tgcgtgccaa ctcccacgct gtttaaccag tctgaaacca
    37141 catcttgcgc gctatgcgca gttaaatcca catgcagcgg cgtgatggag acatagccct
    37201 catctaccgc agcaaaatcg gtccccggac cagcatcaca tttaccgccc ggcgggccaa
    37261 tccagtacag cgtattgccg cgcggatctt gctgcgggat cacctgatct gccggatgtc
    37321 gtgtaccgca gcgcgtcacg cgaatacctt tgatttgatc caagggtaaa tccggaacgt
    37381 taatattaag aatacgcccg gtgcgcagcg gctctttaca cagtgcgcgc aaaattgaac
    37441 aggttaccgc cgcggcagtg tcgtaatgtt tatgcccgtc aagcgagacg gcaagcgccg
    37501 gaaaacctaa atgacggcct tccatcgcgg cggctaccgt accggaataa ataacatcat
    37561 cccccagatt cggcccggcg ttaattccgg acacaacaat gtccgggcgc ggacgcatca
    37621 gagcattcac gccaagatag acgcaatcgg tcggggttcc catttgcaca gcaatatcac
    37681 cattttcaaa ggtaaacgtg cgcagggagg attccagtgt cagagaattt gaagcgccgc
    37741 tgcggttacg atcgggggcg accacctgaa cgtcagcaaa ctcacgcaag gctttcgcca
    37801 gcgtttgtat accgggtgca tgtaccccgt catcattact cagcaatatg cgcataatca
    37861 cctgttgtgt tgataagttc cctgacaacg ctggttgcaa aactacccgc cggaagccag
    37921 aaacggatct ctacggtgac gtcatcccac caattccagc ttaattgttg cggatacagc
    37981 agcatcgctc tgcgcgcggc ttcaactttt tcgcgcacca gtaaagcttg taattcagtt
    38041 tctgcggcga cagctgcttg ttcgaatgcc agcgcttcac gctgagttcc ccattcgcca
    38101 ctgcctggca atgcggcggt tatcatcaac tctttatcgt tgacgcgacg ctgtaattcc
    38161 gccagttctt cggtggttgc gacaaaccag ctaccacgtc cggctaattg tagcgcatcg
    38221 ccgtcaacaa cttgattaac gtctgctttt ttgaggcgct cagcaacaat ctgattaaac
    38281 aacgcactgc gggctgccga caaccaaaaa ctccgtttat tgcgatcgcg caccggagta
    38341 ttggtttgcg cccagcgctg cgccccctgc aagttgctac cgccaatccc aaaacgttgg
    38401 gcaccgaagt agttcggtac accttttacg caaatatcga tcagacgttg ttcaacgtca
    38461 tcgcgattgc tcacttcgcg cagaaccagg gtaaaggcgt tacctttcag cgcgcctaaa
    38521 cgcagcttgc gcttgtgccg cgcatactcc agcacctggc agccttccag ttgaaaggcg
    38581 ctcagatcgg gcatttcctt gcccggcacg cgagcgcata accactgttc cgtaacagca
    38641 tgtttgtctt tttgcccagc gaagctgact tcacgggcat gaattttcag gaatttcgcc
    38701 agtgcatccg ccacaaaacg ggtattgcag ccgtttttga ggattctaac cagaatatgc
    38761 tcaccttcac catcaggctc aaagcccaaa tcttccacca ccacaaagtc ttccggattg
    38821 gctttcagca gcccggtgcc ttgcggttta ccgtggaggt aagtgagatt atcaaactca
    38881 atcattttgt tgccttaatg agtagcgcca ccgcttcaca ggcaatccct tccccacgtc
    38941 cggtaaatcc cagtttttcc gtagtagtgg ctttcacgtt aacatcatcc atatggcagc
    39001 cgagatcttc ggcaataaac acgcgcattt gtggaatgtg cggcaacatc ttcggtgcct
    39061 gagcgatgat agtgacatcg acgttgccaa gggtataacc cttcgcctga atacgacgcc
    39121 aggcttcgcg tagcagctcg cggctatcgg cacctttaaa tgccggatcg gtatccggga
    39181 acagcttgcc gatatccccc agcgccgccg cgccaagcaa tgcatcggtc aacgcatgga
    39241 gcgccacgtc gccatcagaa tgcgccagca atcctttttc gtaaggaatg cgtacgccac
    39301 caatgataat tgggccttca ccgccaaagg catgtacgtc aaaaccgtgt ccaattcgca
    39361 ttatgtattc tcctgatgga tggttcgggt gaggtaaaac tcggccagtg ccaaatcttc
    39421 cgggcgcgtg actttaatgt tatccgcacg gccttcgacc aactgaggat ggaatccgca
    39481 atattccagc gccgaggctt cgtcggtaat agtcgcgcct tcatttagag cgcgcgtcag
    39541 acagtcatgt aacagctcac gagggaaaaa ttgcggcgtc agcgcgtgcc ataagccgtt
    39601 gcgatcaacg gtatgagcaa tggcattttt gcccggttcg gcacgtttca tagtatcgcg
    39661 cactggtgcg gcgaggatcc cccccgtgcg gctggtttcg ctcaacgcca acaatcgcgc
    39721 gaggtcatcc tgatgcaaac aaggacgagc ggcgtcatgc accaataccc actgcgcgtc
    39781 gccagcggct ttcagacctg ccagcacgga atcggcacgc tcatcaccgc catctacaac
    39841 ggtgatttgc ggatgattcg ccagaggaag ttgtgcaaaa cggctatcgc caggacttat
    39901 ggcaatgacg acacgtttca cccggggatg cgccagcagc gcatgcaccg agtgttcaag
    39961 aatggtttga ttaccgattg agagatattg cttaggacat tccgtttgca ttcgacggcc
    40021 aaatccggcc gccggaacca cggcgcaaac atccaaatga gtggttgcca tgttaattcc
    40081 cgggctgatt tatcgattgt tttgccccgc agactgtgcg cgcttcgacg cgtcaggcac
    40141 cagacgataa aaagtttcgc ccggcctggt catgctgagt tcattacgcg cacgctcttc
    40201 gagcgcctcc tggccgccat tgagatcgtc aatttcggca aaaagttgat cgtttcgcgc
    40261 tttaagtttc gcgtttgtag cttgctgtgc cgccacatca tcattgacgc gggtatagtc
    40321 atgtataccg ttcttaccga accacagcga atactgtagc cagaccagaa tagccagcaa
    40381 cagcagcgtt agtttaccca tcctgccccc tgaaaaacgg catcatcatc ccatgcatcc
    40441 gaagacgact ctacatcctc tgttggggat accgcgacaa cgcgggcaaa tgtaccacat
    40501 ttgtccattg ttacgtatac ccagggcgtg cagaacataa tctcattatt agttacggtt
    40561 tgaattatga acagaggaga cgggaaagta caaattagcc cagtagccac ataaacagtg
    40621 cgccaaacat aatgcctact gtcaccaggg tgaaaacaat actgtagcgt agctttccgt
    40681 ccatcaatga atgcagcgca atccccacca ctaccgcgac gggcatcagc gccagaaaga
    40741 aaggccaggt gtagataaag aagaacagcg tgttagagcc ataaatcaac atcggcatcg
    40801 ccagcgcaaa taaccaggag ataaaaccga ccacggcacc aggcagtgac catgtggttt
    40861 cttcatcctc agtaaggctg tcgttatttg ttagtgtaat gttatggcta ttacgcatat
    40921 ttgatcctgt tactttgacg aaccgggcat ggaaacccgg tggtgtctca ggatctgata
    40981 atatcgttct gtctcaacag atctaataat tgctgtacca aatttgttac taattgttca
    41041 ccattgagat gaatttctgc cgattcaggc gcttcgtaaa cggaatctat tcccgtaaag
    41101 ttgcgcagtt caccggcacg cgctttctta tataagcctt tgggatcgcg ggcttcgcaa
    41161 atcgccagcg gcgtatcgac aaacacttcg ataaagcgcc cttctcctac gcgttcgcga
    41221 accatctggc gttcggcgcg gtgtggcgag ataaatgcgg tcagcaccac cagtccggct
    41281 tcaaccatca aattcgccac ttcaccgacg cgacggatat tctctttacg atcggcatcg
    41341 ctaaaaccga gatcgctgca taatccgtgg cgaacattgt cgccatccag cagatacgta
    41401 ctgacgccga gtttatgtaa cgcctcctcc agcgccccgg cgaccgttga tttaccggac
    41461 ccggagaggc cggtaaacca cagcactaca ccacgatgac cgtggtgtag ctcgcgttgt
    41521 tgcacagtga ccggatggct atgccagacg acgttttcgt catgcagcgc cattatttat
    41581 cccccagcaa atcgcgcgcg ccccagtgcg gaaagtggcg acgaaccaga gcattcaatt
    41641 ccagttcgaa tgcactgaat tcagatggcg cagcagttgc ctggctaact ggctcgtgca
    41701 ccataccggc acccacggtc acattgctca ggcgatcgat aaaaatcagc ccacccgtca
    41761 ccggattttg ttgataacga tctaacacca gcggctcgtc aaaagtgaga tccacgaggc
    41821 cgatcccatt cagtggcagg ttttcaactt cacgctgggt aaggttatta atatcaacct
    41881 gatagcgaat gccatcaaca cgcgcgcgcg tcttcttacc ggcaattttg atgtcgtaac
    41941 tctgccctgg agaaagcggc tgttccgcca tccataccac atccaccgac gcgctctgca
    42001 ccgccggtaa cgcttcgtct gccgccagca gcagatcgcc acggctgatg tcgatctcat
    42061 ccgtcagcac cagggtgatc gcttctccgg caaaggcttc ttcgcgatca ccatcaaaag
    42121 tcacgatccg cgcgacgttt gattccacac cagagggcag cactttgaca cgttgcccga
    42181 cttccacgcg accggatgcc agcgttccgg cgtaaccacg aaaatcgaga ttcgggcgat
    42241 taacgtactg caccgggaag cgcattggct gagcatccac cactcgctgg atctccacgg
    42301 tttccagcac ttcgagcagt gtcggaccgc tgtaccacgg catactttca ctttgcgatg
    42361 ccacgttgtc gccttccagt gcagagagcg gcacaaagcg gatatccaga ttacccggca
    42421 gctgcccggc aaaggtcaaa taatcttcac gaatacgggt gaacgtctct tcactgtaat
    42481 ccaccagatc cattttgttg atcgccacga ccagatgttt gatccccaac agtgtggaga
    42541 taaaactgtg acgacgggtt tgatcgagca cgcctttacg ggcatcgatc agtaagatcg
    42601 ccagttcaca tgtcgatgcg ccagtcgcca tattgcgggt gtactgctcg tgccctgggg
    42661 tgtcggcgat aataaattta cgcttctcgg tagagaaata gcggtaggcc acgtcaatgg
    42721 tgatgccctg ttcgcgctca gcttgcaggc cgtccaccag cagagccaga tccagctttt
    42781 cgccctgggt gccgtgacgc ttactgtcgt tatgcagcga tgagagctga tcttcgtaga
    42841 tttggcgggt atcgtgcagc agacgaccaa tcagagtact tttgccgtca tcgacgctac
    42901 cacaggtcag aaaacgcagc aggcttttat gttgttgcgc aatcatccag gcttcgacgc
    42961 cgccttcatt ggcgatttgt tgtgcaagtg cggtgttcat cttaaaaata cccctgacgt
    43021 tttttcagct ccatagaccc cgcctggtcg cggtcaatca cgcggccctg acgttcactg
    43081 gtggtggaaa ccagcatctc ttcgatgatt tccggcagtg tttgtgcatt tgactccacc
    43141 gcaccggtca gcggccagca gcccagcgta cggaaacgca ccatccgttt tttaatcact
    43201 tcgcccggtt gcaggtcgat acggttgtca tcaatcatca tcaacatacc gtcgcgttcc
    43261 agaaccggac gttccgcagc gagatatagc ggaacaatgt cgatattttc cagccagatg
    43321 tattgccaga tatcctgctc ggtccagtta gagagcggga agacgcggat gctttcgcct
    43381 ttgttaattt gcccgttgta gttgtgccac agctccgggc gctgattttt cggatcccag
    43441 cgatggaagc ggtcacggaa agagtaaatt cgctctttag cgcgggattt ctcttcgtca
    43501 cggcgcgcac caccgaaggc ggcatcaaaa ccgtatttgt tcagcgcctg tttcaggcct
    43561 tcagttttca taatatcggt atgtttcgcg ctgccgtgca cgaatggatt aatccccatc
    43621 gccacgcctt ccgggttttt atgcaccagc agttcgcagc cgtaggcttt agcagtacga
    43681 tcgcggaact catacatctc gcggaatttc cagccggtat cgacatgcag caacgggaaa
    43741 ggcagcgtac ctggataaaa cgccttgcgc gccagatgca gcatgacgct ggaatcttta
    43801 ccgatagagt agagcatcac cggatttgag aattctgccg ccacctcgcg aataatgtgg
    43861 atgctttccg cctccagttg ccgcaggtga gtaagtcgta tttgatccat aaccgttcct
    43921 ttgcaatacc gctattttct tgccatcaga tgtttcgact atagggagcg taagagaacg
    43981 aatgaaatta ccaattagaa tgagtagttc cttaacggaa taacgatttg gcaaagctaa
    44041 tatcaaaaag tgcttaaggc accggatttc gggcgtttag gaagatttga aattgtttta
    44101 gcgcagcggc agtttcatac tatggcggta aaaaaatttg catggtattt aaggactcac
    44161 tatgttttcc gcattgcgcc accgtaccgc tgccctggcg ctcggcgtat gctttattct
    44221 ccccgtacac gcctcgtcac ctaaacctgg cgattttgct aatactcagg cacgacatat
    44281 tgctactttc tttccgggac gcatgaccgg aactcctgca gaaatgttat ctgccgatta
    44341 tattcgccaa cagtttcagc aaatgggtta tcgcagtgat attcggacat ttaatagtcg
    44401 gtatatttat accgcccgcg ataatcgtaa gagctggcat aacgtgacgg gaagtacggt
    44461 gattgccgct catgaaggca aagcgccgca gcagatcatc attatggcgc atctggatac
    44521 ttacgccccg ctgagcgatg ctgacgccga tgccaatctc ggcgggctga cgttacaagg
    44581 aatggatgat aacgccgcag gtttaggtgt catgctggaa ttggcagaac gcctgaaaaa
    44641 tacgcctacc gagtatggta ttcgatttgt ggcgaccagc ggcgaagagg aagggaaatt
    44701 aggcgctgag aatttactca agcggatgag tgacaccgaa aagaaaaata cgctgctggt
    44761 gattaatctc gataacttaa ttgttggcga taaattgtat ttcaacagcg gtgtaaaaac
    44821 ccctgaagca gtaaggaaat taacgcgcga cagggcgctg gcaattgcac gcagtcacgg
    44881 aatagccgca acgaccaatc cgggtttgaa taaaaattat ccgaaaggca ctgggtgttg
    44941 taatgacgca gaaatattcg acaaagcggg cattgctgta ctttcggtgg aagcgactaa
    45001 ctggaatctt gggaataagg atggttatca gcaacgcgca aaaacagccg cattccctgc
    45061 gggaaatagc tggcatgacg taagactgga taatcaacag catattgata aggcacttcc
    45121 tggaagaata gaacgtcgct gccgtgacgt tatgcggata atgctacctc tggtgaagga
    45181 gttggcgaag gcgtcttgat gggtttgaaa atgggagctg ggtgttctac cgcaggggcg
    45241 gggaattcta agtgatatcc atcatcgcat ccagtgcgcc cggtttatcc ccgctggcgc
    45301 ggggaacact ttaaaagcat cagctaaata attaaagaaa tcggtttatc cccgctggcg
    45361 cggggaacac gatccccacg ataatcaccc tgggatcaat ttcggtttat ccccgctgac
    45421 gcggggaact cctatctgat tttgaataca catctctaaa acacggttta tccccgctga
    45481 cgcggggaac tctctaaaag tatacatttg ttcttaaagc attttttccc ataaaaacaa
    45541 cccaccaacc ttaatgtaac atttccttat tattaaagat cagctaattc tttgttttta
    45601 aacaggtaaa aaagagacca accttaaacc atccaaatct accggagtac gcctgtttac
    45661 cccaaatgtc tggaactcaa atcccgattc cgtattcgta gcccatgcca tcaccacatt
    45721 gccttcttcc gccagtccag atatttgttc ccagatcatt tcacggattc ttgcggatac
    45781 aacacctaca tacacccctg cacgtacctc caacaaccag atggctaatc tgcctcgtaa
    45841 gcgcggaggt acattttcag tgaccacgac caacatactc atttcagcta ctccgatggc
    45901 ctgcatctcc cagtgaaaca ggaagcggaa tggcaacagg ctgtgcatct tcaggtgggg
    45961 ccggcggttg tatttctcca gcagcaagca cgtcctctat aagcggaatc aatttggcta
    46021 atgttttact actgcgaaaa atatccctgc acgccaaacg gacttcccgg tccggctcac
    46081 caggattacg acgcgctatc tcaaaagctt tcggtacaac agtgtcaaat ttaatgatgt
    46141 ccgcgatatc gtaaacaaag gaaagaggct ttcctgtatg cacaaaccca atagctggtg
    46201 cataaccagc tgcaagtatc gccgcttcag ttacgccgta taaacaggaa gttgcagcgc
    46261 taatgcattg gttgaccgta tcgccctttt cccagtcttt cggatcgtag cgacgtccat
    46321 tccatgtcac gccgtattgc ttcgccagaa gtgcgtaggt tgcccgcacg cgactgcctt
    46381 ctatacctct gagttgctct acggagcgcc gggcaggcgc aggttctcca aaccgaagtt
    46441 caaacatttt acgtacgacc ttcagacgca aatcttcatc cagagcaagt tttgcctgat
    46501 agagcagctt atctgaacgc gcaccaccag gctgaccaga agcataaaca cgaacgcccg
    46561 cttcccccac ccataccaac aatgttccaa cttgcgcagc caggcgtaca gctgcatgcg
    46621 aaacccgggt accaggttcc agcatgatgc aggcaaccga gccaacagga atatgagtgc
    46681 ggatccctgt cttgtcgata agtacaaacg cgccatctat tacatcgatc tgcccatatt
    46741 gcagaaagat catggagacg cgatctttga gtggaatggg gttaagagga agccaagcca
    46801 ttttattaca cctcaatcac agtggagcca aagatagcaa accacatccc atcgatttgg
    46861 ctggcccaat accttgctgt aaaagatcta ttaacgctgg cgcatcgtta atggtgagca
    46921 caccttcaaa gcaaaccgtt tggatttttc catttttacc ttccccagaa aaatactgtg
    46981 gccgttccga tatgggatgc acatcttcaa tgcgcgccgc attgcccaat ttacgttgca
    47041 accacgcgat ttgttctgct tcttttatta acggaacccg acagcgttta atattccctt
    47101 tactgtccag gcgcttttga ttgtcgagaa tagttttgat cggatttgcc cgaagccgaa
    47161 aatagagtgg aacaccaacc tgaagttgaa attcaacctg tttagtttta atgactgtcg
    47221 caacggcagt tgaaacaggc atttgcgctg actgcaataa aacatgacag ccttctggtg
    47281 tgtttcgctt ctcaacatga aaaagaaaat cacgagcagc atccggtctg tttggaaata
    47341 aatgccataa tccctggtga agttggtaaa gatccctgct ccaggccctg gcaatgatga
    47401 ttttactgag atacatccat acctccttta atcacatacc attctcggga agcaaattgt
    47461 cgaggtaagg tgatcatcgg ttcgtcgcgc gccgtaaatt ttaaatgatg ccctgtaact
    47521 gattcctcac tatatatatc gccgccaacg ggctcataat ttaatagcgc cttctgagga
    47581 tccgatgcct ggcatgttgc aagaaaaagc gggtgtgtta gtgggcaact tctccgcccc
    47641 agatacggtg tataccgagg ctttaatact gctttttcaa gttctgacat aaccatcgtt
    47701 gcctgtggtg ttaaccagat agcgatggta aaggaggcat cacataaata ttcgcgccat
    47761 gtttgaatcg tttcatgact tttcaaacca cggtaatctt ctcgcgctcc aaggactgta
    47821 tggtaatcac gcaaccccgt tacagacaca cgacgatcgt caagaatgag ttcatcgcag
    47881 cgcactgcaa attgcacact ctctgataac gcctgtaatg aagaagtatc atcacgttgg
    47941 atcccaagac aagccccgag tagccctaat aacccgcttc gggtcggaaa tcttccggta
    48001 ggtcgcgttc cttcaaaggt cggctgtccc caagcttgca ttggcccagc aagtcgcaat
    48061 atcaaataag atttcatgtt cacgcctcgc cattattacg aacccaggac tttaactgtt
    48121 ctaaagtagg catttgctgt acctgaccag taatagggtc tacatcagac aagctgaatt
    48181 gcgcagcggc tccgttcaga ccatatccat tggcaacgcg atcccaatat tgattaaacg
    48241 cctgtaaaga cggttgcaaa aagccatctt tcgctttcac cgctttttca aaagcatttg
    48301 ccatagaaag tggcatatcg gagaaattaa ccattaccat atccgcaggg ttaaaagcgg
    48361 cataagtacg ctgttttgct ccagggacct ctgttgccag catatgaaca acatgggttg
    48421 caatttccag agcctgctcc ctggaggcac cgcctaaatt ttcctgaagt tgagcgaggt
    48481 taatattggc ataacgataa aaaacacccg atgaaaattc ctgtgttccc agatgtgcag
    48541 aaccttgttc ctgtaaatca tctacggcgg tgaaccagtc aatatcagaa tcaacctgat
    48601 gggtagtgat cgcatgcgca atggacattg caccatcaac ttttcccaac tcactcatca
    48661 tgccgctggt tgccattctt ccactaagcg caatatcaac accctgctgt aaattcacac
    48721 gtatggcggc aatatcttcc ttaagaactt tgagcagctt tttatcatcc agattatcag
    48781 cctctgcttt tgcaacctgc tcacagaacc aggctatttc tcccacaacc cagggagtaa
    48841 ccgcatcggc agaaatcttt tcggcttcat caactgattt accggagagc agagctaatg
    48901 tcttatcgat gatttttcgc tcaaaacgtt caccaagttt ttgccgaaga acatcacgta
    48961 attgtgcaag atgaattgtt ctgagactgg attcaccaat attttgtgca taataatcac
    49021 ttttacgcat cgcacgttta aggctttgac ttgaaattct tactcgtctt ttcccgccga
    49081 aaatagcgtc tttctgcatg ttcatatcgt cgcggttcag acatgaaggg ctgtgagaga
    49141 tcagaacatg aatattgata aagttagaca tagaaaggtt tccttacgca tttttgtttg
    49201 tggtcaatac aaaatcttcc agaagttgct ggcgttcgcg ctttccccac caggtcaaca
    49261 tcctggccat taatggccag tcaagtacgg gttcggcgtg agtaagtaat cgacgtaact
    49321 ggaccatatc ggctgttctg tcagcccgaa ttaattgaaa gatacggcgc tcgttaattc
    49381 ttccactatt ggctaaagct cttcccaacg agatacctgt tgtttgctcc ggttttttgt
    49441 cctgatgtcg gatgacattc tttcctgcgc tcaggcaaaa caccatgcgc aaaagagcct
    49501 gctggtgacg tgggttttcc caaccaaaag gttgcaccag cctataaaac gcagggatat
    49561 cgcgtaattc atcaggttct gaaacacgtc taatttgcgc acatgctcca ttatccagtt
    49621 gttgccaggc tcgatataaa gccattgcat caatttcatc agccatttga tggccctcct
    49681 tgcggtttta actcccgtaa atgtttgtat agcgtggcgc gggcaagcgc taatgtgctt
    49741 attaatttag gatgatgtgc atagggcgct acagattgat taaatagcat ttcacacaat
    49801 tgatgaagtt tgtctcgtaa atcagctatt acctcatcag cctgggaaaa attaatattc
    49861 gccagtacat cgagaattaa taattcactc tggcgataga aatgcctttc tgcagtctca
    49921 tgaacagaga ctccggcccc tttgaagtct ttatttttaa acccttctgc aaaggtatat
    49981 aacgccttgc gtaaggctgt tttatatccc aaaccaacag tcactatttc gtttatcaca
    50041 ttgccgtatt gttgccatcc ctgattaaac atcagcacat catgacgccg ttcaagaata
    50101 gatgcttgat tattacgata tccccccata atcaattcaa gaggactttg cgacgcaata
    50161 tttctgaatt gattcacaac cgccgccacg cgatttccat tttcattttg aataatttta
    50221 tctaccacaa ctcggctgat ttgtgtccat gatggtgcgg aggtggtgaa agcaagaaat
    50281 ttttcctcaa cctccccttt cttgactgtt accagacaag gggaatgcgg atggggccat
    50341 agcccattaa ctgtaaaggt aaatttttcc ttaagaaaac cggtataacg caaattgctt
    50401 tcctgtccac agcaagaaca tttaccaatc ccaatgggat cgcataattc aatatgcgct
    50461 ggttgccaga ataagccacg gacaaagcca attgacgaag ctggcacaga ttcattgggc
    50521 ttgacaggct taatccaggt aggttggttt tccgtatgtg attcattggg aaattgtttt
    50581 tggagacgag gtattgtgag gacattgagt aacaccgtcg agcgaagatc gatcccacgt
    50641 acgaaggtgg ttataggtgt tcctccacgt aaaccgcttt taaagccacc accaaaacca
    50701 ggtgcctgat tcgcctggtt gaataacgca atcgcagtgc atccaccaca taatgcttca
    50761 ccctgacccg gttgattgac aaatgcacaa ttcgtcgcgc cgcttacccc agccaacagt
    50821 ttttccattg gagtcacatc atttgcttta acacctttgg tctgcataaa gggatgttct
    50881 gcgtgattaa ggtagaacat atctatccac ggcgcgatga gtcgttgaaa ctcatcttca
    50941 ctgagcggat tcataatgcg atgccgaaat tcaacgtcat cttttgcagg ggcgataatt
    51001 tgcccgatgc aaaccagcag agctaaagcg gccagttcca tatcgtcacg gggcaaactt
    51061 aaccgccact gatttttact gcagaatagc gattgcagat ttatgatttg gactttcccc
    51121 ccactttgcg ggcgtacagg gatccagtta tcaataagca aattcatttg ttctccttca
    51181 tatgctccga catttctcct gcatttctat acgtcggcac acttccgtta tcggtaaaaa
    51241 caaagaaaaa atacggcgta attaattcaa taatcacatt cac
//
LOCUS       DAJOSY010000018        49184 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJOSY010000018 DAJOSY010000000
VERSION     DAJOSY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.018"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..752)
                     /locus_tag="OHN27_001168"
     CDS             complement(<1..752)
                     /locus_tag="OHN27_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCQ2285650.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARIVEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMQF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTAIIFSQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNPHDLNYSQVSCKNSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYMCNESISYLLGE"
     gene            complement(875..1327)
                     /gene="tabA"
                     /locus_tag="OHN27_001169"
     CDS             complement(875..1327)
                     /gene="tabA"
                     /locus_tag="OHN27_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCQ2285651.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1472..2065)
                     /locus_tag="OHN27_001170"
     CDS             complement(1472..2065)
                     /locus_tag="OHN27_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCQ2285652.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2136..2849
                     /gene="bdcA"
                     /locus_tag="OHN27_001171"
     CDS             2136..2849
                     /gene="bdcA"
                     /locus_tag="OHN27_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2285653.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHRQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2980..3375
                     /locus_tag="OHN27_001172"
     CDS             2980..3375
                     /locus_tag="OHN27_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCQ2285654.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            3656..3790
                     /gene="pyrL"
                     /locus_tag="OHN27_001173"
     CDS             3656..3790
                     /gene="pyrL"
                     /locus_tag="OHN27_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCQ2285655.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3794..4729
                     /gene="pyrB"
                     /locus_tag="OHN27_001174"
     CDS             3794..4729
                     /gene="pyrB"
                     /locus_tag="OHN27_001174"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCQ2285656.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4742..5203
                     /gene="pyrI"
                     /locus_tag="OHN27_001175"
     CDS             4742..5203
                     /gene="pyrI"
                     /locus_tag="OHN27_001175"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCQ2285657.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNAVLAN"
     gene            5276..5662
                     /gene="ridA"
                     /locus_tag="OHN27_001176"
     CDS             5276..5662
                     /gene="ridA"
                     /locus_tag="OHN27_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCQ2285658.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5868..8564)
                     /gene="mgtA"
                     /locus_tag="OHN27_001177"
     CDS             complement(5868..8564)
                     /gene="mgtA"
                     /locus_tag="OHN27_001177"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCQ2285659.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8705..8758)
                     /gene="mgtL"
                     /locus_tag="OHN27_001178"
     CDS             complement(8705..8758)
                     /gene="mgtL"
                     /locus_tag="OHN27_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCQ2285660.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8943..9890
                     /gene="treR"
                     /locus_tag="OHN27_001179"
     CDS             8943..9890
                     /gene="treR"
                     /locus_tag="OHN27_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCQ2285661.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10009..11430
                     /gene="treB"
                     /locus_tag="OHN27_001180"
     CDS             10009..11430
                     /gene="treB"
                     /locus_tag="OHN27_001180"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCQ2285662.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11480..13135
                     /gene="treC"
                     /locus_tag="OHN27_001181"
     CDS             11480..13135
                     /gene="treC"
                     /locus_tag="OHN27_001181"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCQ2285663.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALT
                     DESSVFYTYQKLIALRKQEAVLTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRG
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            13529..15667
                     /gene="nrdD"
                     /locus_tag="OHN27_001182"
     CDS             13529..15667
                     /gene="nrdD"
                     /locus_tag="OHN27_001182"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCQ2285664.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDKWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15827..16291
                     /gene="nrdG"
                     /locus_tag="OHN27_001183"
     CDS             15827..16291
                     /gene="nrdG"
                     /locus_tag="OHN27_001183"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCQ2285665.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPDKDIW
                     VWTGYKLDDLNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16336..16722)
                     /gene="cybC"
                     /locus_tag="OHN27_001184"
     CDS             complement(16336..16722)
                     /gene="cybC"
                     /locus_tag="OHN27_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCQ2285666.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDTLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16777..18129)
                     /gene="pmbA"
                     /locus_tag="OHN27_001185"
     CDS             complement(16777..18129)
                     /gene="pmbA"
                     /locus_tag="OHN27_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCQ2285667.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSADPCAGVADKELLAFEAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18223..18774
                     /locus_tag="OHN27_001186"
     CDS             18223..18774
                     /locus_tag="OHN27_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCQ2285668.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18930..20303)
                     /gene="mpl"
                     /locus_tag="OHN27_001187"
     CDS             complement(18930..20303)
                     /gene="mpl"
                     /locus_tag="OHN27_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCQ2285669.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESNFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20479..21477
                     /gene="fbp"
                     /locus_tag="OHN27_001188"
     CDS             20479..21477
                     /gene="fbp"
                     /locus_tag="OHN27_001188"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCQ2285670.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21510..22505)
                     /gene="yjfF"
                     /locus_tag="OHN27_001189"
     CDS             complement(21510..22505)
                     /gene="yjfF"
                     /locus_tag="OHN27_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCQ2285671.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22492..23514)
                     /locus_tag="OHN27_001190"
     CDS             complement(22492..23514)
                     /locus_tag="OHN27_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2285672.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(23528..25030)
                     /locus_tag="OHN27_001191"
     CDS             complement(23528..25030)
                     /locus_tag="OHN27_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCQ2285673.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25170..26126)
                     /gene="ytfQ"
                     /locus_tag="OHN27_001192"
     CDS             complement(25170..26126)
                     /gene="ytfQ"
                     /locus_tag="OHN27_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCQ2285674.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26436..26966
                     /gene="ppa"
                     /locus_tag="OHN27_001193"
     CDS             26436..26966
                     /gene="ppa"
                     /locus_tag="OHN27_001193"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCQ2285675.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27346..27687)
                     /locus_tag="OHN27_001194"
     CDS             complement(27346..27687)
                     /locus_tag="OHN27_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCQ2285676.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(27690..31469)
                     /gene="tamB"
                     /locus_tag="OHN27_001195"
     CDS             complement(27690..31469)
                     /gene="tamB"
                     /locus_tag="OHN27_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCQ2285677.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDVGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(31466..33199)
                     /gene="tamA"
                     /locus_tag="OHN27_001196"
     CDS             complement(31466..33199)
                     /gene="tamA"
                     /locus_tag="OHN27_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCQ2285678.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            33405..34043
                     /gene="msrA"
                     /locus_tag="OHN27_001197"
     CDS             33405..34043
                     /gene="msrA"
                     /locus_tag="OHN27_001197"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCQ2285679.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34366..35709
                     /locus_tag="OHN27_001198"
     CDS             34366..35709
                     /locus_tag="OHN27_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCQ2285680.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(35771..35977)
                     /locus_tag="OHN27_001199"
     CDS             complement(35771..35977)
                     /locus_tag="OHN27_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCQ2285681.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36302..36859
                     /locus_tag="OHN27_001200"
     CDS             36302..36859
                     /locus_tag="OHN27_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCQ2285682.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(36849..37589)
                     /gene="cysQ"
                     /locus_tag="OHN27_001201"
     CDS             complement(36849..37589)
                     /gene="cysQ"
                     /locus_tag="OHN27_001201"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCQ2285683.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNIMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37779..39722
                     /gene="cpdB"
                     /locus_tag="OHN27_001202"
     CDS             37779..39722
                     /gene="cpdB"
                     /locus_tag="OHN27_001202"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCQ2285684.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYISAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNNSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPDNTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMVNANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39845..40225)
                     /locus_tag="OHN27_001203"
     CDS             complement(39845..40225)
                     /locus_tag="OHN27_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCQ2285685.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40314..41174
                     /gene="qorB"
                     /locus_tag="OHN27_001204"
     CDS             40314..41174
                     /gene="qorB"
                     /locus_tag="OHN27_001204"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCQ2285686.1"
                     /translation="MIAITGATGQLGHYVIKSLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSETDFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNK"
     gene            41283..42248
                     /locus_tag="OHN27_001205"
     CDS             41283..42248
                     /locus_tag="OHN27_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2285687.1"
                     /translation="MINGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            42356..43018
                     /gene="ytfE"
                     /locus_tag="OHN27_001206"
     CDS             42356..43018
                     /gene="ytfE"
                     /locus_tag="OHN27_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCQ2285688.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43063..44475)
                     /gene="cycA"
                     /locus_tag="OHN27_001207"
                     /gene_synonym="dagA"
     CDS             complement(43063..44475)
                     /gene="cycA"
                     /locus_tag="OHN27_001207"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCQ2285689.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44784..45404)
                     /gene="fklB"
                     /locus_tag="OHN27_001208"
     CDS             complement(44784..45404)
                     /gene="fklB"
                     /locus_tag="OHN27_001208"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCQ2285690.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLQGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45623..46261
                     /gene="ytfB"
                     /locus_tag="OHN27_001209"
     CDS             45623..46261
                     /gene="ytfB"
                     /locus_tag="OHN27_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCQ2285691.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIILVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46245..46892)
                     /locus_tag="OHN27_001210"
     CDS             complement(46245..46892)
                     /locus_tag="OHN27_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCQ2285692.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            47105..47896
                     /locus_tag="OHN27_001211"
     CDS             47105..47896
                     /locus_tag="OHN27_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCQ2285693.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTIRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            47906..48748
                     /locus_tag="OHN27_001212"
     CDS             47906..48748
                     /locus_tag="OHN27_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCQ2285694.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPQGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGNRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            48758..>49184
                     /locus_tag="OHN27_001213"
     CDS             48758..>49184
                     /locus_tag="OHN27_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCQ2285695.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVP"
BASE COUNT        12075 a        13062 c        12284 g        11763 t
ORIGIN      
        1 ttttcaccaa gtaagtatga aatcgattca ttacacatat aaggcattaa tagcagatgt
       61 tctttcctta ttgctaataa tgcattgttc aatggttttt ctaataaatc cgtaatcgta
      121 gaatcaactg agtttttaca actcacctgc gagtaattta agtcatgagg attatttaac
      181 tcctgcggaa tatttaatag acgagctatt tccttgttag cttcgcgttg gtaacacgcc
      241 agaatcttgt tagtaatcag gtgtaatgaa ctctcatctt ttctttcaaa ggattttcct
      301 tcataacgat tagtcgaacc atcaggttgt gaaaatataa tggctgtatg tccttcacga
      361 atcccattat taaaagctga ggcaagtgta atttctattt ccaccttaga taaatccgct
      421 cgctcccctt caggattatt cataacaaac tgcattattt cactgccatc attatttttg
      481 atataagatg aggactcaat atgagccatc atatttctac ccgcttcgtt ggagcgtttg
      541 ctaaaatcca cataacccag ggtaaatatt tttaccaaaa actccaaaaa tgtcttagga
      601 tcaaggatat ttcttgtgtt atcctgcccc tcgacaattc gggcaaaatt attcattcca
      661 acacgccagg tatttaaaac ttcacttttt tgtttaaaat tgtcggctaa tgtaatgtgt
      721 tgagaataat tcaaatctga tattttgctc atgacggact ccttgagtta ataagaacgt
      781 ctaagcatta caaattctac tcacaaaaaa atatcaaaaa acgctctttt cacaaactcg
      841 ccgtaaacct ggagttattt acggcgaaaa gtcatcacgc catcagcatc ttcactactg
      901 ctttgcgaac ctgggctggc gcgcccactg cgcacagcgg tttatgcact tcccccggat
      961 aaaacacaac aaaatcacct tcattaagga taactgtttt ctcatcaacg ccttccggca
     1021 aaaatgcgat gtctttatca gctaaccagt cggtatccgg cgtgcctgca ggttgcgtgc
     1081 tgaaggtcat gccttcctga ccttttaaca caatctgaat gtcgagatag cgggcatgat
     1141 actccgcacg gcgagcttcg tacggctcgg tcatatcttc cgagataaga taaaacagac
     1201 gattgccttc gatatcgtgc ttaccctttg gcgtttctgc cgtaacgtgc gctttgatat
     1261 gctcaatcgc ctggcgtaac tcctggggta gccacggctg aagattatga atatttccga
     1321 tgatcatctt ttccactcct taattaaaac aatgtttcat ttctatcgtt atacgaaaag
     1381 atgatcaatt ccgccagcct taacgcgtga attattgtct tatcacaact tatcggcagg
     1441 cgcgttcccg cctttcggat caaggccagc atcactcctt gagaagagtt tttatcgctt
     1501 cccccgccag tgcagctgtt gcacagagtt gttcgattga gtgcccctcc cgtgccttcg
     1561 cagaaagccc tgacatcacc gtactcataa aatcagtcac acattgtgcg ctttgtggat
     1621 gccgcctggc gatatagtca taaatggtcg tttctgcggc gtgataatat tgaacggcaa
     1681 tatcacgcgc tagtggatca tgactatgaa taccttcaag aaccatacag ccagcgcagc
     1741 cgccattttg gctatatctg cgcgccgctt cttttaatac ctcaaccagg cactcgccta
     1801 ctggacgatc atcacgaaga atatcggcaa gcggaatagc ttccgtaccg acgtattcat
     1861 tgagtacacg gctaaataac ccagctttac tgccaaaagc cgcgtagagg ctcggggggt
     1921 taataccaag ataatcagta acttcagcaa cactgacagc atcgaaacct ttttggtgaa
     1981 aaagcacttt tgccgcagag actgcctgct caggagcgaa acgtctggga cgacctggaa
     2041 cgcgagattg ttttttagtg accattacaa aacctgttga cagaaagtta aaacagtttt
     2101 gtaatgcatg ttacataata aatcaaggag tccttatggg cgcttttaca ggtaagacag
     2161 ttctcatcct cggtggcagt cgtggtatcg gtgccgctat cgtacgtcgt ttcgtcaccg
     2221 atggggccaa tgtacgattc acctatgcgg ggtcgaaaga tgccgctaaa cgcctggcac
     2281 aagagactgg agcgacagca gtattcacag atagtgctga cagagacgct gtcattgatg
     2341 tcgttcgtaa gagcggcgca ttggatatcc tggtggtaaa tgcaggtatt ggcgtctttg
     2401 gcgaggccct ggaattaaat gccgacgata ttgatcgcct tttcaaaatc aatattcatg
     2461 ctccttatca tgcctctgtt gaagccgccc ggcagatgcc cgaaggcggg cgcatcttaa
     2521 tcatcggctc cgtgaatggc gatcgtatgc ctgttgcagg catggctgct tatgccgcca
     2581 gcaaatctgc cctgcaaggc atggcgcgcg ggctggcccg tgattttgga ccgcgtggga
     2641 tcaccattaa cgtcgtccag ccagggccaa ttgataccga cgctaatccc gccaacgggc
     2701 caatgcgcga tatgttgcat agtttgatgg ctatcaaaag acataggcaa ccggaagagg
     2761 tcgctggtat ggtcgcatgg ttagcagggc cagaagccag ttttgttacc ggcgcgatgc
     2821 ataccattga tggcgcgttt ggcgcataac cgactacgct caattaagcc cagccattat
     2881 ccatgatgtc tgggttttgt ttactcacgt cgtccgctaa aagcggctcc cggtaaagag
     2941 aaatcttctg ttcatgtcgt aaatcttgag gataaccaaa tggtagaaag aaccgctgtt
     3001 ttccctgctg gccgacattc actatatgct gagcatcgtt attctgcggc tattcgttcc
     3061 ggcgatttgc tgtttgtttc cgggcaagta ggaagtcgag aggacggaac accagaaccc
     3121 gattttcagc aacaagtcag actggcattt gataatttgc atgcgaccct ggcagctgcg
     3181 ggatgcactt ttgacgatat cattgatgtt acgagcttcc ataccgatcc agaaaaccaa
     3241 tttgaagaca tcatgacggt gaaaaatgaa atatttagcg ccccacctta tccaaactgg
     3301 acggcggtgg gtgttacatg gctggcaggc tttgattttg aaattaaagt gatagcgcgc
     3361 atccctgagc agtaagcaat agtgttagcc gttcgctttc acactccgcc ctataagtcg
     3421 gatgaatgga ataaaatgca tatctgattg cgtgaaagtg aaaaagaaaa aagcagggaa
     3481 tgtctgcaat tattgatacc gaacgacagt tcccctgcag aatcacatca aataaaaatg
     3541 catatacctt gacttttaat tcaaataaac cgtttgcgct gacaaaatat tgcatcaaat
     3601 gcttgcgccg cttctgacga tgagtataat gccggacaat ttgccgggag gatgtatggt
     3661 tcagtgtgtt cgacattttg tcttaccgcg tctgaaaaaa gacgctggcc tgccgttttt
     3721 cttcccgttg atcacccatt cccagcccct caatcgaggg gctttttttt gcccaggcgt
     3781 caggagataa aagatggcta atccgctata tcagaaacat atcatttcca taaacgacct
     3841 tagtcgcgat gaccttaatc tggtgctggc gacagcggcg aaactgaaag caaacccgca
     3901 accagagctg ttgaagcaca aagtcattgc cagctgtttc ttcgaagcct ctacccgtac
     3961 ccgcctctct ttcgaaacat ctatgcaccg cctgggtgcc agcgtggtgg gcttctccga
     4021 cagcgccaat acatcactgg gtaaaaaggg cgaaacgctg gccgatacca tttcggttat
     4081 cagcacttac gtcgatgcga tagtgatgcg tcatccgcag gaaggtgcgg cgcgcctggc
     4141 caccgagttt tccggcaatg taccggtact gaatgccggt gatggctcca accaacatcc
     4201 gacgcaaacc ttgctggact tattcactat tcaggaaacc caggggcgtc tggacaatct
     4261 ccacgtcgca atggttggtg acctgaaata tggccgcacc gttcactccc tgactcaggc
     4321 gttagcgaag ttcgacggca accgttttta cttcatcgcg ccggacgcgc tggcaatgcc
     4381 gcaatacatt ctggatatgc tcgatgaaaa agggatcgca tggagtctgc acagctctat
     4441 tgaagaagtg atggcggaag tagacatcct gtacatgacc cgcgtgcaaa aagagcgtct
     4501 ggacccgtcc gagtacgcca acgtgaaagc gcagtttgtt cttcgcgcca gcgatctcca
     4561 caacgccaaa gccaatatga aagtgctgca tccgctgccg cgtgttgatg agattgcgac
     4621 ggatgttgat aaaacgccac acgcctggta cttccagcag gcaggcaacg ggattttcgc
     4681 tcgccaggcg ttactggcac tggttctgaa tcgcgatctg gtactgtaag gggaaataga
     4741 gatgacacac gataataaat tgcaggttga agctattaaa cgcggcacgg taattgacca
     4801 tatccccgcc cagatcggtt ttaagctgtt gagtctgttc aagctgaccg aaacggatca
     4861 gcgcatcacc atcggtctga acttgccttc tggcgagatg ggccgcaaag atctgatcaa
     4921 aatcgaaaat acctttttga gtgaagatca ggtagatcaa ctggcattgt atgcgccgca
     4981 agccacggtt aaccgtatcg acaactatga agtggtgggt aaatcgcgcc ccagtctgcc
     5041 agagcgcatc gacaatgtgc tggtctgccc gaacagcaac tgtatcagcc atgccgaacc
     5101 ggtttcttcc agctttgccg tgcgaaaacg cgccaatgac atcgcgctca aatgcaaata
     5161 ctgtgaaaaa gagttttccc ataatgcggt gctggccaat taattgcggt tggtaataaa
     5221 agtctggctc cctataatga gccagacttt ttaccgctgt aataaaggag aaatcatgag
     5281 caaaactatc gcgacggaaa atgcaccggc agctatcggt ccttacgtac agggcgttga
     5341 tctgggcaat atgatcatca cctccggcca gatcccggta aatccgaaaa cgggcgaagt
     5401 accggcagac gtcgctgcac aggcacgtca gtcgctggat aacgtaaaag cgatcgtcga
     5461 agccgcaggc ctgaaagtgg gcgacatcgt taaaaccacc gtgtttgtga aagatctgaa
     5521 cgacttcgca accgttaatg ctacctacga agccttcttc accgaacaca atgccacctt
     5581 cccggcacgt tcttgcgttg aagttgcccg tctgccgaaa gacgtgaaaa ttgagatcga
     5641 agcgatcgct gttcgtcgct aatcttgatg gaaatccggg ctatcatgcc cggattaagt
     5701 ctgatgacaa acgcaaaatc gcctgatgcg ctacgcttat caggcctacg tgattcctgc
     5761 aatttattga atttgttggc cggataaggc atttacgccg catccggcat gaacaaagct
     5821 tactttgtct gcaatctgaa tccaggccac catgcccagt tgattcttta ttgccagccg
     5881 taacgacggc tataaaatcc tttcaccagt tgggttaacg tcatataccc tgccagaatc
     5941 gcaaccagcc acgggaaata gcttaacggc agcgcctgca gctgcagata actggccagc
     6001 ggtgaaaatg gcaatgcgat cccgacaatc atcacgatca cggtcatgat cattaacggc
     6061 cacgatgcac agctctgaat aaacggcaca cggcgggtgc ggatcatatg cacaatcagc
     6121 gtttgtgaca gcaagcccac cacaaaccat cccgactgga acagcgtttg cgtttccggc
     6181 gtgttggcat ggaataccca ccacatcagg caaaacgtca gaatatcgaa gatcgagctg
     6241 atcggtccga agaagatcat aaagcgcccc agatccgccg ggttccaacg ctgcggcttt
     6301 tgaatttgct cgtcgtcgac gttatcaaac gggatcgcca cctgtgacac atcatacagc
     6361 aggttctgaa taagcaagtg taacggcaac atcggcagga agggcaagaa agcactcgct
     6421 accagcacgc tgaacacatt accgaagtta gagctcgccg tcattttgat gtatttcagc
     6481 atgttggcga aagtgcgacg tccctcaata accccctctt ccagcaccat caggcttttt
     6541 tccagcagga tgatatcagc cgcttcacgg gctatatcta ccgcgccatc cacagaaatg
     6601 ccgatatccg cagcgcgtaa ggcgggcgca tcattaatac catcgcccat aaagccaacc
     6661 acatgccctt cgcgcttcag taaggtcaca atgcgttctt tatgcatcgg cgtcagacga
     6721 gcaaacagcg tggtgcgctg tgcgagattt gccagttcgt cgtcagatag cgtttcaata
     6781 tcactaccaa tgaccacctc tcccgcgtcc agccccactt catggcacac tttcgctgca
     6841 actaactcac tgtcgccagt aaggattttt acggtaattc cactcgcttt taatgccttc
     6901 agtgccggag cagttgtctc ttttggcgga tcaaggaaag caatatatcc ttcgaggatc
     6961 aggtcggatt catccgcccg ctggtaatct ccttcacgcg ctggcaggta tttcgtcgcc
     7021 accgcaacca cgcgcagccc ctgacgatta agcgtatcag taacccgctt aatcttacgc
     7081 agcatgatgt catcgagcgg cacaatctcg ccattgtgac gcacctgcga acacacattg
     7141 aggatttcct gcaatgcacc tttgcaaacc agctgatggt gctcggtatt ttctgccact
     7201 accaccgaca tccggcgacg ctcgaaatca aacggaatct catcaatttt ttgccaacgg
     7261 ctggccagag agcgcgctga ctcttcatcc gtaccttcga gcaccgctgt atcgagcagg
     7321 tttttaagtc cggtctgata atgactgttc aaccacgcgc tatgcagcac gcgttcgctg
     7381 gttttaccgg agatatcggt atgattctcc agcacaattt tgtcctgtgt cagggtgcca
     7441 gttttatcag tgcacagaat atccattgcg ccaaagttct gaatagcatc cagatgtttg
     7501 acgatcactt tctgtttcga cagctttact gcgccgcgcg ccagcgtcga ggtaacaatc
     7561 atcggcaaca tttccggcgt taagcctacc gcgaccgaaa gcgcaaacag tgccgcttcc
     7621 caccagtcgc ctttggtgta accattgatt aacagcacca ccggagccat caccagcata
     7681 aagcgaatca gcagcatact gacgcggctg atcccttgct gaaaggcatt cggctcgctt
     7741 tcctgctcac taacacgccc cgccagttga ccaaaccagg tattggcacc tgtagcaatc
     7801 accattgctt gtgccgtgcc gctcaccacg gtggtgccca taaaacacag ggtgtcgcac
     7861 tccagcggat tgctgtgctc cggctggcga gtggtagctg ctttttctac gggcagagac
     7921 tcaccggtta acgacgcctg agcgacgaac agatcccgcg cctgcaagat acgtaaatct
     7981 gccgggatca tatcccccgc cgccagtttt ataatatcgc cgggcaccag ctggtcgatc
     8041 gggatctcca gccagccatt ttcgcctttg tcgttaatta cgcgcagcac cgtcgcagta
     8101 ttgctgacca tcgctttcag ggcatctgcc gctttagtgg aacgtgcttc ctgaataaag
     8161 ttcagcaacg tagaaatagc gaccattagc gcgataacgc ccgcggcaaa taaatcttcc
     8221 gtggcgtaag aaatagcgcc gagaatggtg agtaaaatat taaatgggtt gcgatagcag
     8281 acccataaat gtacccacca cggcgacggt tgttgtgcgg gtaatttatt ttcaccatgt
     8341 tgttcgcggg cagattccac ttccgcctga tttaaccctt ccggatgggt gtcgaacgtt
     8401 ttccataatt cttcttcggg catcaccgcc attttcaggc aatgcgcact taaggacggc
     8461 gggaccgccg tattcactgt ctgctgcgcg ccaggcaatg gatcgcgatg aactagacgg
     8521 gaaggtaaat ggcgaatgag ccgggtaaaa atttctttaa acataaggag tccctccgca
     8581 ctgtctgaaa cagtacagaa atgtcttcag gcgtggcgaa gccttacctg aaggtaataa
     8641 agctgagcag ggacgttaaa acgtcacggc cttacgcatc cggtaacgcc gtgaaaagac
     8701 gggcttaccg gaaaagtttt aatctccgtc gagggagagg cgtgggatca ggttccatat
     8761 aacctccggt aagtgaaaaa agcgccgcaa ataataagga gaatccaata atattcgtag
     8821 catctatggc gatataatac ctgctggcaa ctaaaccaac gataaaccag actttaccca
     8881 ttgctgaatg cacgggtaaa gttaggctca aataattaaa caacacgtta caggacaaca
     8941 ggatgcaaaa tcggctgacc atcaaagaca tcgcacgctt aagcggcgtg gggaaatcta
     9001 cagtttcccg ggtgctgaat aacgaaagcg gcgtgagcca gcgcacccgc gagcgtgttg
     9061 aagcagtgat gaatcagcat ggattttccc cttcccgctc tgcgcgcgct atgcgtgggc
     9121 aaagcgataa agtggtcgcc atcattgtta cccgtctgga ttcgttgtca gaaaatctcg
     9181 ccgttcaaac catgctgcca gcgttctatg aacaaggtta cgacccaatc atgatggaaa
     9241 gtcagttttc cccacaatta gttgccgaac atttgggggt gctgaaacgg cgtaatatcg
     9301 acggcgtagt gctgttcggt tttactggca taacagaaga aatgttagcc cactggcagt
     9361 catcgctggt tctgctggcg cgtgacgcaa aaggctttgc ttcggtctgt tatgacgacg
     9421 aaggggcaat caagatcctg atgcaacggc tgtatgacca ggggcatcgt aatatcagtt
     9481 atctcggcgt gccgcatagt gacgtgacaa ccggtaagcg acgtcacgaa gcctacctgg
     9541 cgttctgcaa agcgcataaa ctgcatcccg ttgccgctct gccagggctt gctatgaagc
     9601 aaggctatga gaacgtggca aaagtgatta cgcctgaaac tactgcgtta ctgtgcgcaa
     9661 ccgacacgct ggcacttggc gcaagtaaat acctgcaaga gcaacgcatc gacaccttgc
     9721 aactggcgag cgtcggtaat acaccgttaa tgaaattcct ccatccggag atcgtcaccg
     9781 tagatcccgg ttacgccgaa gctggacgcc aggcggcctg ccaattgatc gcacaggtaa
     9841 ccgggcgcag cgaaccgcaa caaatcatca tccccgccac cctgtcctga tcgtttcctg
     9901 aacgataaat tgtgatcttc gctgcgcttc gggaacgttc ccgtttttaa atttttccgc
     9961 gcaatatatt ctgcagccaa ccaaaaatgt catctgccat ggggctttat gatgagcaaa
    10021 ataaaccaaa cggatatcga tcggttgatt gaactggtcg gcgggcgcgg caatattgcg
    10081 acagtgagcc actgtattac tcgcctgcgc tttgtcctca accaaccggc caatgccaga
    10141 ccgaaagaaa ttgagcaact ccccatggtg aaaggctgtt tcaccaatgc cgggcaattt
    10201 caggtggtga ttggcaccaa tgtgggtgat tactatcaag cactaatagc gtcaaccgga
    10261 caggcgcagg ttgataaaga gcaggtaaaa aaagccgccc ggcagaatat gaaatggcat
    10321 gagcagttga tctctcattt cgcggagatc ttcttcccgt tgctgcccgc gttgattagc
    10381 ggcggtttga tcctcggttt tcgcaatgtg atcggcgatt tgcccatgag caacggtcaa
    10441 acgctggcgc aaatgtaccc ttccctgcaa acgatctacg attttctgtg gttgatcggt
    10501 gaagcgatct tcttctacct gccggtcggg atttgctggt cagcggtgaa aaaaatgggc
    10561 ggcacgccga tcctcggtat cgtgcttggc gtgacactgg tttctccaca gctgatgaac
    10621 gcttatctgc tcgggcagca gctgccggaa gtgtgggact ttggcatgtt cagcatcgcc
    10681 aaagtaggct atcaggcgca ggtgatcccg gcactgttag ccggactggc gctgggcgtt
    10741 attgaaactc gccttaaacg catcgtgccg gattacctct atctggtggt ggtacccgtc
    10801 tgttcgctga tcctcgcggt gttcctcgcc catgcgctga ttggtccgtt tggtcgcatg
    10861 attggcgatg gcgttgcctt tgcggtacgt cacctgatga ccggcagctt tgctccgatt
    10921 ggtgcggcat tgtttggctt cctgtacgcg ccgctggtga tcaccggcgt acaccagacc
    10981 acccttgcta ttgatttgca gatgattcaa agcatgggtg gcacgccagt gtggccgctg
    11041 attgcgctgt cgaatatcgc tcagggctcc gccgtgatag gcattatcat ttccagccgc
    11101 aagcacaatg aacgcgagat ctccgtgcct gccgctatct ccgcctggct tggggtcact
    11161 gagcctgcaa tgtacggcat caacctgaaa tatcgcttcc cgatgctgtg cgcgatgatt
    11221 ggttctggtc tggcaggatt gctatgcggc ctgaacggcg ttatggcgaa tggcatcggc
    11281 gtaggcggcc tgccgggaat tctctcgatt caaccgagct actggcaggt gtttgcgctg
    11341 gcaatggcta tcgccatcat catcccgatt gtactcacct cgtttatcta tcagcggaaa
    11401 taccgcctgg gcacgctgga cattgtttaa ttttcttcgg ggcgcaattg cgccctctcg
    11461 catttgcagg aataacgtaa tgactaatct tccccactgg tggcaaaacg gcgttatcta
    11521 ccagatttat ccaaagagtt ttcaggacac cacgggtagc ggcaccggcg atttacgtgg
    11581 cgttatccaa cgcctggact atctgcataa actgggcgtt gatgccatct ggctaacccc
    11641 cttttatgtc tctccccagg tcgataacgg ttacgacgta gcgaactata cggcgattga
    11701 tcccacttac ggcacgctgg acgattttga cgaactggtg acgcaggcaa aatcgcgcgg
    11761 gattcgtatc attctcgata tggtgtttaa ccatacctct acccaacatg cctggtttcg
    11821 cgaggcgctg aacaaagaaa gcccttaccg ccagttttat atctggcgcg atggcgaacc
    11881 agaaacgcca ccgaacaact ggcgttcaaa atttggcggt agtgcgtggc gctggcatgc
    11941 ggaaagcgaa cagtactatt tgcatctctt tgcaccagag caggcggatc tcaactggga
    12001 gaatccagcg gtacgcgcag agctgaaaaa agtctgtgag ttctgggccg atcgtggggt
    12061 cgacgggttg cgcctggatg tggtgaatct gatctccaaa gacccacgtt tccctgatga
    12121 cctggatggc gacgggcgtc gcttctacac cgacgggcca cgagcacacg agtttttgca
    12181 cgagatgaac cgcgatgtgt ttacgccacg cgggttaatg accgtaggtg aaatgtcctc
    12241 caccagcctt gagcattgcc agcgatacgc ggcactgaca ggcagtgaat tgtcgatgac
    12301 ctttaatttt catcacctga aggtcgatta tcccggtggt gaaaaatgga cgctggctaa
    12361 acctgacttt gtggcgttga aaaccttgtt ccgccactgg caacaaggaa tgcacaacgt
    12421 agcatggaat gccttgttct ggtgtaacca cgatcagccg cgcattgttt ctcgctttgg
    12481 tgatgaaggt gaataccgcg tgcctgcggc aaaaatgctg gcgatggtgc tgcatggcat
    12541 gcagggaacg ccgtatatct accagggcga agagattggc atgaccaacc cgcatttcac
    12601 gcgcattact gactatcgcg acgtggagag cctcaatatg tttgccgagc tgcgcaacga
    12661 tgggcgtgat gccgacgagt tattggcaat cctcgccagt aaatcccgtg acaacagtcg
    12721 cacgcccatg caatggagca acggcgataa tgccgggttt acggctggcg aaccgtggat
    12781 tggcctgggc gataactatc aacaaatcaa cgtagaagcc gcgctgaccg atgagtcctc
    12841 ggtgttttac acctaccaaa agttaatcgc actgcgtaag caagaagccg ttctgacatg
    12901 gggcaattac caggatctgc tgccaaacag ccctgtattg tggtgctatc gccgtgaatg
    12961 gaaggggcaa accttgctgg tcattgccaa ccttagccgt gggatccaac cctggcagcc
    13021 agggcaaatg cgcggcaact ggcagcttgt gatgcataac tacgaagaag cctcaccaca
    13081 accctgtgcc atgaatttac ggccttttga ggctgtctgg tggttacaga agtaaatctt
    13141 ccctaagccc cggtaatgcc ggtcattccg gggttttgtc tgtcagcatg ttgtttttgt
    13201 tgatttttca accagcaaat tcattaaaaa atttacataa cgctgtagcg cccgtcatcc
    13261 gtacgctctg ctttttactt tgagctacat caaaaaaagt tcaaacatcc ttgatgcaaa
    13321 gcactatata tagactttaa aatgcgtccc aacccaatat gttgtattaa tcgactataa
    13381 ttgctactac agctccccac gaaaaaggtg cggcgttgtg gataagcgga tggcgattgc
    13441 ggaaagcacc ggaaaacgaa acgaaaaaac cggaaaacgc ctttcccaat ttctgtggat
    13501 aacctgttct taaaaatatg gagcgatcat gacaccgcat gtgatgaaac gagacggctg
    13561 caaagtgccg tttaaatcag agcgcatcaa agaagcgatt ctgcgtgcag ctaaagcagc
    13621 ggaagtcgat gatgccgatt attgcgccac tgttgctgcg gttgtcagcg agcagatgca
    13681 gggccgcaac caggtggata tcaacgagat ccagaccgca gttgaaaacc agctgatgtc
    13741 gggtccatac aaacaactgg ctcgtgctta catcgagtac cgtcacgatc gcgacattga
    13801 acgtgaaaaa cgcggtcgcc tgaaccagga gatccgtggt ctggtcgagc agaccaacgc
    13861 ctcgttactc aacgaaaacg ccaacaaaga cagcaaggtg attccaaccc agcgcgacct
    13921 gctggccggg atcgtggcta aacactatgc acgccagcac ctgctgccgc gtgacgtggt
    13981 gcaggcacat gagcgtggcg atattcacta tcacgatctc gattactcgc cgttcttccc
    14041 gatgttcaac tgcatgttga ttgacctgaa aggcatgctg acccagggct ttaaaatggg
    14101 gaacgccgag attgaaccgc cgaagtcgat ctctacggca accgctgtaa ctgcgcagat
    14161 tattgctcag gtcgccagcc atatttatgg cggcaccacc attaaccgta tcgatgaagt
    14221 gctggcaccg tttgtcaccg ccagctacaa caaacatcgc aagaccgcag aagagtggaa
    14281 catcccggac gccgaaggct acgctaactc tcgtaccatt aaagagtgct acgatgcctt
    14341 ccagtcgctg gagtacgaag taaacaccct gcacaccgcc aacggtcaga cgccgtttgt
    14401 aacctttggt tttggtctgg gcaccagctg ggaatcgcgc ctgattcagg aatctatcct
    14461 gcgtaaccgt atcgcaggcc tcggtaaaaa ccgtaaaact gcggtgttcc cgaaactggt
    14521 gtttgcgatt cgcgatggtc tgaaccataa aaaaggcgat ccgaactacg acatcaaaca
    14581 gctggcgctg gagtgcgcaa gcaagcgcat gtatccggat atcctgaact acgatcaggt
    14641 agtgaaagtc accggttcgt ttaaaacccc gatgggctgc cgcagcttcc tcggcgtgtg
    14701 ggaaaatgaa aacggcgagc agatccacga tggtcgtaac aacctcggcg tgatcagcct
    14761 gaacctgccg cgtattgctc tggaagcaaa aggcgatgaa gccaccttct ggaagctgct
    14821 ggatgaacgt ctggtgctgg cacgtaaggc gctgatgacc cgtatcgctc gtctcgaagg
    14881 cgtgaaagcg cgcgtggccc cgatcctcta tatggaaggt gcttgtggcg tgcgtctcaa
    14941 tgctgacgat gatgtttctg aaatcttcaa aaacggtcgt gcgtctattt cgctgggtta
    15001 catcggcatc cacgaaacca ttaacgcgct gttcggcggc gagcatgttt acgacaacga
    15061 gcagcttcgc gcgaaaggta tcgccattgt tgaacgtctg cgtcaggcag tggataagtg
    15121 gaaagaagaa acgggttatg gtttcagtct ctacagcacg ccgagtgaaa acctgtgcga
    15181 ccgcttctgc cgtctcgata ctgctgagtt tggcgtggtg ccgggcgtga ccgataaagg
    15241 ttactacacc aacagtttcc acctcgatgt ggagaagaag gtgaacccgt acgacaagat
    15301 cgactttgaa gcgccttacc cgccgctggc gaacggtggt ttcatttgct acggcgagta
    15361 tccaaacatt cagcacaacc tgaaggcgct ggaagatgtc tgggattaca gctatcagca
    15421 tgtaccgtat tacggcacca atacaccgat tgatgagtgc tacgagtgtg gctttaccgg
    15481 tgagttcgag tgcaccagca aaggcttcac ttgcccgaaa tgtggtaacc atgacgcctc
    15541 ccgtgtgtcg gtgactcgcc gcgtgtgcgg atatttaggt agcccggatg cacgtccatt
    15601 taacgccggt aagcaggaag aagttaagcg ccgcgttaaa catttgggga atgggcagat
    15661 aggttaatct tcactctacg ttaagcaaat gccggatgcg gcgtaaacgc cttatccggc
    15721 ctacatttgg caacggctgt gtaggcctga taagacgcga cagcgtcgca tcaggcattg
    15781 attaccggat gcagcctaca cgcggactga taagccaggc gaaataatga attatcatca
    15841 gtactatcct gtcgacatcg tcaacggccc cggcactcgc tgcaccctgt ttgtctccgg
    15901 gtgtgttcat gaatgccccg gttgctataa caaaagcaca tggcgggtaa attctggtca
    15961 gccatttacc aaagcaatgg aagaccagat cattaacgat ctgaatgaca ctcgtatcaa
    16021 acgccagggg atttccctct ccggcggcga tccgctgcat ccgcaaaacg tgccggatat
    16081 tctgaaactg gtaaaacgca tccgcgccga gtgtccggat aaagacatct gggtgtggac
    16141 aggctataaa ctcgacgatc tcaacgctgc gcaaatgcag gttgttgatt tgattaacgt
    16201 actggtcgac ggcaaatttg tgcaggattt aaaagaccct tccctgatct ggcgcggcag
    16261 cagcaatcag gtggtgcatc atttgcgatg ataagttccc tgttacctga gtgcaggcaa
    16321 cagggaaatg aggaattaac gatacttctg gtgataggcg ttgcgggtcg ttttcagttg
    16381 ctctgcggca gcctgcgctt cttttacttt accttcattt gccagcttca gcgcgtcgtc
    16441 aatctgacca accagaatat cgaaaccgtg acggaagtct ttcatttccg ggctgtccgg
    16501 tgatttatct tcgagcttcg gcggcgttgc tttttgcgca tccagcgctg cggcgcgcat
    16561 cttcgttaac gcgtctttga cttgcgccgc gttatcggct ttttcgacca cttttaaagt
    16621 gtcgttgagg gtttccatat tgtcttcaag atcagcggca aacgacgccg aactgaatac
    16681 caacgaggag actgcaagaa tagctaacag gcttttacgc attgctcact tcctttttta
    16741 ttatgaagat ttggcgcggc aggtcgcgcc agttttttac tgcccggcga ttttcatctc
    16801 cggcaacagc acagaaccac actgtatatt actgcgtgtt tcaatatcgt taccgacggt
    16861 gacaatattg cgccacatat cttttaaatt acctgcgatg gtgatttcgc taaccggata
    16921 ctgaatttcg ccgttttcta cccagaagcc cgccgcacca cgggaataat caccggtaat
    16981 tgcactcacg ccctggccca tcagctccgt caccaccagc ccggtgccca tctctttgag
    17041 catttgttcg aagcttagcc cttgtccggc aatccgccag ttgtgaatac cgcccgcatg
    17101 tccggtgctt ttcagcccca gttttcgcgc cgaatagctg gtcagcagcc actgagtcag
    17161 gatgccatct ttaataatat cgcgacgctc ggtgcgcaca ccttcgctgt caaatggcgt
    17221 cgatgccagc cctttcagca gatgcggatg ctcttcaatg gtcagccagt ccggcagaat
    17281 ttgtttaccc agcgaatcca gcaggaaggt agatttacgg taaaccgctc cacccgctat
    17341 cgcccccacc agatgcccaa aaagcccggt tgccacttca ttggcaaaaa tgaccggcgc
    17401 tttcatggtg gagagtttac gcggtgacag gcgcgataaa gtacggcgag cacagtcggc
    17461 cccaacccac tctggcgttt gcagatcgct catcgcacga ccaatggtgt aggcgtaatc
    17521 acgctccata tcgccatttt cttcggcaat tacacagctg gagagcgaat gacgcgtaga
    17581 gcagtaaccc tgcaacatgc cgtggctgtt gccaaaaact ttgacaccgt agtggctgtt
    17641 aaagctgcca ccttcggtat tggtgatacg tttgtccgcc tgtaatgctg cctgttctgc
    17701 gcgggccgcc agttcaatgg cttcatccgg ggaaacttcc gcagggtgga acaaatcgag
    17761 atccggtgcc tcaaaggcca gcagctcttt gtctgccacg ccggcacagg gatctgccga
    17821 ggtataacgg gcaatatcca gcgccgcctg tacggtgcgg gcaatggcct gcgggcttaa
    17881 atcggtggat gatgcgctac ctttgcggtt ctggtgataa acagtgatcc ccagcgcgcc
    17941 atcgctattg aattcaacat tctccacttc accataacgc gtgcttacgc taatgccggt
    18001 ggtcttgctg acggcaactt ccgcaccgtc cgatttgcct gaggccaact ccagcgcagt
    18061 ggaaactgct tcttccagaa tcttgcgctg cgcttcaact tgagagatta ctttcattgc
    18121 aagtgccata atgtagagag tttctctgaa gtctaacaga gaaccgtttt tcagtgcgca
    18181 tcttaactgg taacattagc ctcttttttt aaggagcctg agatgactaa gcagcccgaa
    18241 gactggctcg acgacgttcc cggtgatgac atcgaagacg aagacgatga aattatctgg
    18301 gtcagtaaaa gtgaaattaa acgtgatgcc gaggagctaa aacgccttgg cgcggaaatt
    18361 gttgatctgg ggaaaaacgc gctggataag atcccgttag atgcggatct gcgcgcggct
    18421 attgagctcg cccagcgtat taagatggaa ggtcgccgcc gccagttgca gctcatcggt
    18481 aagatgctgc gccagcgcga tgtagaaccg atccgtcagg cgctggataa gctgaaaaac
    18541 cgtcacaacc agcaggtggt gctgttccat aaactggaaa acctgcgcga tcgcctgatc
    18601 gatcaaggtg atgacgccat cgctgaggta ttaaatctgt ggcccgatgc cgatcgtcag
    18661 caactgcgta ctctgatccg taacgcgaag aaagagaaag aagggaataa gccgccgaaa
    18721 tccgcacgcc agattttcca gtatctgcgc gaactggcgg agaatgaagg gtaattttcg
    18781 ttgttgatgt gagtatggtt tgccggatgc ggtgcacctg taggcctgat aagacgcatc
    18841 aagcgtcgca tcaggcacta tgctcaaatg ccggatgcgg cgtgaacgcc ttatccggcc
    18901 tacgcacact atatcaggct gaggccgaat tactgcgcag cttccgcctt cttcgccaga
    18961 ccatccagca gtttctgatg gatcccacca aaaccgccgt tgctcatcac cagaatatgg
    19021 tcgccaggct gagcggtttt caccaccata tctgccagcg tatccacatc gccactccag
    19081 tgtgcaggct gaacgcaggc ttctgccact tctgccacct gccacggaat atgcgccggt
    19141 tgcagcagga agacttcatc ggcacgacct aatgaaggtg ccagatcgtc tttgcagatc
    19201 cccattttca tggtattcga gcgcggttcc agcacagcaa taatgcgcgc cgtaccacca
    19261 actttgccac gcagcgccgc cagcgttgcc agaatcgccg tcgggtgatg ggcaaaatcg
    19321 tcatataccg tgacgccatt cgcttcacca cgcaactcca gacggcgacg agcattaata
    19381 aacgaaccca gcgcgttagc ggcatctgcc ggcgctacac caacatggcg agccgctgca
    19441 atcgccatca ggccattgtg catattatgt tcgcctacca gcgaccattt cacttcgccc
    19501 actttttcgc catccagcaa aacttcccat tcggaagcat cggtggtcag ctttttcgcc
    19561 tgccagtgac cctgctcgcc caccagctcc tgctcgctcc agcagcccat cgccatggtc
    19621 tgtttcaggt tgatgtcgtt ttctggccag ataatacggc cctgccctgg aacgatacgc
    19681 accagatggt ggaactgttt ctggatcgct ttcaggtcgt caaagatatc ggcgtgatcg
    19741 aactcaaggt tgttgaggat cagcgtacgc gggcagtaat gaacaaattt agagcgtttg
    19801 tcgaagaagg cgcagtcata ctcatccgct tcgataacaa agaagttgct ttcgcccaga
    19861 cgcgccgaaa cctcaaagtt ccccggcaca ccgccgatca caaatcccgg tttgtaaccg
    19921 cactgttcca gaatccaggt cgccattccc gcggtggtgg ttttgccatg tgtaccggca
    19981 acggccagca cccagcggtc gcgcagcaca aaatcgtgca gccactgtgg acctgacata
    20041 taagggatgt ttttttccag taccgcttcc acacacggat ttccacgggt catggcgttg
    20101 ccaataatca ccagatccgg ctgcggatcg agctggctgg catcgtaacc ctgaatcagt
    20161 tcaatgcctt gcttctcaag taaggtgctc atcggcggat acacattggc gtccgaaccc
    20221 gttacttcat ggcctaactg gcgcgccagc atcgccagac cgcccataaa cgtgccacaa
    20281 attcctaaaa tatgaatgcg catacgtcac tatccttttt taatctgccg ctcattttac
    20341 gcatatgtcg ggcaagtgag aaacgcattt caggataatc cgcaatttgc tggcgcgatt
    20401 cacctgagcg caacattccg attatttgtt aagattgtta cggtcgcttt actccataaa
    20461 cattgcaggg aaagttttat gaaaacgtta ggtgaattta ttgtcgaaaa gcagcacgag
    20521 ttttctcatg ctaccggtga gctcactgct ttgctgtcgg caataaaact gggcgccaag
    20581 attatccatc gcgatatcaa caaagcagga ctggttgata tcctgggtgc cagcggtgct
    20641 gagaacgtgc agggcgaggt tcagcaaaaa ctcgacttgt tcgctaatga aaaactgaaa
    20701 gccgcactga aagcacgcga tatcgttgcg ggcattgcct ctgaagaaga agatgagatt
    20761 gtcgtctttg aaggctgtga acatgcaaaa tacgtggtgc tgatggaccc actggatggc
    20821 tcgtccaaca tcgatgttaa cgtctctgtc ggtaccattt tctccatcta ccgccgcgtt
    20881 acgcccgtcg gcacgccggt aacggaagaa gatttcctcc agcctggtaa caaacaggtt
    20941 gcggcaggtt acgtggtata cggctcctct accatgctgg tttacaccac cggatgcggt
    21001 gttcacgcct ttacttacga tccttcgctc ggcgttttct gcctgtgcca ggaacggatg
    21061 cgcttcccgg agaaaggcaa aacctactcc atcaacgaag gaaactacat taagtttccg
    21121 aacggggtga agaagtacat taaattctgc caggaagaag ataaatccac caaccgcccg
    21181 tatacctcac gttatatcgg ttcactggtc gcggatttcc accgtaacct gctgaaaggc
    21241 ggtatttatc tctacccaag caccgccagc cacccggacg gcaaactgcg tttgctttat
    21301 gagtgcaacc cgatggcatt cctggcggaa caagcgggcg gtaaagcgag cgatggcaaa
    21361 gagcgtattc tggatatcat cccggaaact ctgcaccagc gccgttcgtt ctttgtcggc
    21421 aacgaccata tggttgaaga tgtcgaacgc tttatccgtg agttcccgga cgcgtaatct
    21481 cctgcgcctc tccacacgga gaggcgtttt tatcgctgcg caatgttgac tcttgtcact
    21541 ggcgaactct gacgattctc ccacagcacc gttaatccac gctgtaatgc tataaaaata
    21601 aacaacaaaa taccgatggc gattttcgtc caccatgagc tcagcgtgcc atcaaagttt
    21661 atgtaagtct gaatcagtcc ctgaatcgcc acgccaaaaa gcgtccctaa taccgttcca
    21721 acgccaccgc tcagaagcgt accgccaatt accactgagg cgatagcatc cagttccaca
    21781 cctacgcccg ccagcgcata tccggcctgg gtataaatcg agaagacaat ccccgccagc
    21841 gttgccagtc cggtggagag catataaatg cgaatagtgg tgctgcgagt ggaaatcccc
    21901 atcaggttcg ccgacgttgc gttgccgcca atggcgtata cctgattacc aaaacgggta
    21961 cgatgcgcga ggaaaatacc gataaccacc accgccaaca tcagcagtcc catcgcactt
    22021 aagcgaccgc cgccagggat tttccacgca aggcttgaga gcgtgtcata aatgggatgg
    22081 tttatcggaa tcgactcttc cgaaacgaga tagctgacac cgcgcaaaaa gaacatcccc
    22141 gccagggtaa tgataaatgc cgggatcttc agggcgtcga tcaaaagccc cataaatgcg
    22201 ccgaaggcac agcccatcac cagcactagc ggaaacgcca gtagcggcga gaggccgaaa
    22261 tcgccaatca cttttgccag aaacacgcca gtaaaggcga tcaccgaacc gacggagaga
    22321 tcgatcccac cggagaggat cacaaaggtc atgccaacgg caatgatccc aagaaaggcg
    22381 ttatcggtca ggatattgca gatcactctt gtggaagcaa aaccgggaaa ctgggtcagg
    22441 cagtaaagat aacccaacac aaagacgccg atggtgatca tcagtggcaa attacgtttt
    22501 atcacggcta cgtactcctt taatcagact gataaagcgt tgcgactgga caatcagcac
    22561 gcaaagaacc accaccgctt ttacaacctg gttcatctct ggcggaaagc ccgaaagcaa
    22621 aattccggtg ttcatcccct gaataatcag cgcccccacc accgaaagca gtaggttaaa
    22681 acgtccgccc atcagcgatc cgccgccaat aaccaccgcg agaatggcgt ccagctccag
    22741 ccataacccg gcgttattgg catcggcacc gcgaatatcc gccgccacga taatgcccgc
    22801 aatcgccgca cacagcccgc tcaacacgta ggtaagcatg acgatgatcc gcgtgtttac
    22861 cccggcattt tttgccgccc gaatgttgat accaacggct tcgataaaca tccccagcgc
    22921 cgttttgcgg gtcaacagcc agaacaggat aagcgtcagc accgcgataa tgaccggcgt
    22981 tggcaggaac aacagcgatc cactgccaaa ccatgagaga tccggcgagt taaacgtgac
    23041 gatctgtccg gaggtgatca gttgcgccac gccgcgcccg gcgaccatca ggatcagggt
    23101 ggcaacaaac ggctgaattt tgaggatcgc taccagtatg ccgttccaca atcccgccag
    23161 gatgccagtg cccagggcgc ttaacaaaac aatcggcagg ctgaatcccg cgaccgtcat
    23221 cgcagccgtt gtggctccgg cgatagccat caccgccccc acggagagat caatcccacc
    23281 tgttgcgatc accagcgtca ttccgatagc caacagcgca acgggggccg cacggttaag
    23341 aatgtctatg gggctaccga ataaacgccc atcctggagc accacctgcc agaaatgcgg
    23401 ggccaccagg ctatcgacca gcagcaccag caacagtgcc gccagctgcg gcattcccgt
    23461 tggccagcga aagcgccttt tcggcggcgt agtgtccggg agagattgag gcatcacact
    23521 gttctcctta cgccgcaatg gcgttcatga tcgccggaac ggaaagctct gccagcggga
    23581 tctccgccac ctgtttgcga tcgcgcatga tgatcacccg gtcggcatag cccaccagtt
    23641 cttccagttc ggaggagatc accagcagcg ccagaccatc ggcgcatagc gtttcaatca
    23701 ggcggatgat ctcggcgtgg gcaccaacat caatgccgcg agttggctca tcaaggatca
    23761 gaaattgcgg tcgggtcagt agccaacgtg aaagcaacac tttttgctga ttgccgccag
    23821 agagaaattc aatcggttgt tcagttgaag gtgtgcgaat gccaagctgg cggataaagc
    23881 gttcggcaat ctcttgctgt tctttgcggg aaatgggacg tagccagccg cgctgggcct
    23941 ggagagcgag gatgatattt tcccgcaccg aggcggcagc gatgatgcca tcggttttcc
    24001 tgtcttccgg gcagaagcca atgcccagta ccgaagcctg atgtggcgat cgcaggtttt
    24061 gcggtttgcc tttgatcaac gccgtgccgc tgtcagcagg tttgataccg aagatcactt
    24121 cggcggtttc ggtacgtcct gatcccagca atccagccag accgacgatc tcgccggggc
    24181 gtacttcgag atcaaacggt gcgatcgttc cttttttgcc gtaatttttg aacgcggcaa
    24241 cgggtttgtc gctcaacaat gttcgcccgg cacgctgtag cgcgtgggta tccagctcgc
    24301 gccccagcat catttttacc agttcgatct gcggtagctc gcacgtttcc cgacagccta
    24361 cgaaactgcc gttgcgtaag acggtgatcc gatcgctgac ctgatagacc tgatcgagaa
    24421 agtgagtgac aaaaatcagg ctgacgccgc gatcgcgcaa ctgacgcatc aggtcaaaca
    24481 gtaactccac ttcctgggtg tcgagactgg cggtgggttc atcgaggatc agcactttgg
    24541 cagagagatc gatagcccgg caaatagcga cgatttgctg catcgcgact gaaaagcggt
    24601 tgagcggttc gcgcacgtcg agggagaaac cgtaagatgc catcagttcg gtggcgcgct
    24661 tttccatctc tttgcggcgt agaaggccga agcgtttggg ttcgcggcct ataaatagat
    24721 tatcagcgac cgacatattg ggtagcaggt ttacttcctg atagacggtg ccgataccga
    24781 gttgttgtgc atgggcggta tttttcggtg agatagcctg gccttccagc cagatggtgc
    24841 cgcgatcggc gtggtataca ccagttaatg ctttgattag cgttgatttt cctgccccgt
    24901 tttcaccgag cagcgccatg atttcgccgc ggcgcaggct gaaatcaacg ttgtctaacg
    24961 ctttgacgcc ggggaaaaat ttacttaatc cttcggtgcg gaggatctcc tggtgttggt
    25021 tagtcgtcat ggtttccccc ggtgtaaagc gtgctcccag taggcatgat aagacgcggt
    25081 aagcgtcgca tcaggcttca gcgcaccacc gccggatgcg gcgcgagcgc cttatccggc
    25141 ctacgaatcg gcatcaggca tagcaaccct caatacccca tatttttctt cttctctaac
    25201 tcttcttttg cggtatcagg cagataaagg gtggatttgg tcagcgtcag cttttcaggc
    25261 atggtgccgt cttttttgta tttctccagc gcgtcgaagg cgggacctgc catattcggc
    25321 gtcagttcaa cactggcgtt cgcttcgcca tcaatcatcg ctttgtagat atccggtacg
    25381 ccgtcgatag aacctgtcag aatatctttg cctggtttca ggcccgcttc tttaattgcc
    25441 tgaattgcac caatcaccat gtcgtcgtta tgggcgtaaa ccatgcagat gtttttgccg
    25501 ttgttttccg ctttgataaa gctctccatg acttctttgc ctttactgcg ggtgaagtca
    25561 cctgactgcg agcggataat tttgatattt ggcgcattct taatggcttc ggcaaagcct
    25621 ttcttacggt caatggcgac gctggcccca acagtgccct gcagctccac cacgttgcat
    25681 ggtttgccat tcacttcttt taccagccag tcaccaatca acttgccttc gagaatgttg
    25741 tcggcagtga cggtggtcat atagagagat ttgtctttca catcaataga acgatcgagc
    25801 aagaagaccg ggatttcggc atctttcgcc tcttttaata ccggttccca accagtcgca
    25861 accaccggag cgataaagat cgcatccacc ccttgcgcga cgaaggaacg taccgcttta
    25921 atctggtttt cctgcttttg ctgaccatcg gcaattttca gcgtaattcc gcgtttttcg
    25981 gcctcacttt tcgccacatt ggtttctgcg gcgcgccagc cagattccga tccgacctgc
    26041 gaaaatccta cggttaatgg agcggccaac gccatagacg acatggctgc cgagactgca
    26101 gagactacaa gtaagcgttt ccacataggg ttgtcctcgt cggggtaatt attattggtg
    26161 aaaagatgtt cgcgaaaaaa ctatagacaa ttcgttatgt aacggattgc gttacatcac
    26221 acttcaaaga agtaaataaa acgttaatca caagtttgta atcgctttca tctcactatg
    26281 aaaaatgcgg ctacggttat ggattttcct gctctgtata ccgtcttaaa attggcgaaa
    26341 aaggaaaatg aagacgaaaa caagcgaaga cattcggcgc gagttggcta taatactcgg
    26401 cacttgtttg ccacatattt ttaaaggaaa cagacatgag cttactcaac gtccctgcgg
    26461 gtaaagatct gccggaagac atctacgttg ttattgagat cccggctaac gcagatccga
    26521 tcaaatacga aatcgacaaa gagagcggcg cactgttcgt tgaccgcttc atgtccaccg
    26581 cgatgttcta tccgtgcaac tacggttaca tcaaccacac cctgtctctg gacggtgacc
    26641 cggttgacgt actggtcccg actccgtacc cgctgcagcc gggttctgtg atccgttgcc
    26701 gtccggttgg cgttctgaaa atgaccgacg aagccggtga agatgcgaaa ctgattgctg
    26761 ttccgcacac aaagctgagc aaagaatacg atcacattaa agacgttaac gatctgccag
    26821 agctgctgaa agcgcagatc gctcacttct tcgagcacta caaagacctc gaaaaaggca
    26881 agtgggtgaa agttgaaggt tgggaaaacg cagaagccgc taaagctgaa atcgttgctt
    26941 ccttcgagcg cgcaaagaat aaataagttc ttctagcgca ataaccctga acgccgggct
    27001 tcggttagta agggtttttt tatgcccgcg ataaataaac tctctattcc accatcatta
    27061 ttctcagcgg ttgcaaggct tgaacggtaa gaacaagcaa acccgaccac cattttgctg
    27121 ttcatagcca cttgctggaa gttagccgac ctcactcata ctcaccgttc aaagcgtgcc
    27181 cccgttttac ccctgcacat ttgtgaacgc cagcagagtt ttttatgcga ttgcagatat
    27241 ggaaagcggc tcggagagaa aagcaaaaga tggaaaagtt acaaaaaaaa atcgcgagat
    27301 acatcgcata aaacaacgcc acccgaaggt ggcgtgcttt tattattact tatccctgtc
    27361 taaccagtcg ccgctttcaa ttagctttaa tccatcgacg ggtcgttgat aaacgtacat
    27421 ccatgcactc ccgtacggcg tctgaatcaa ctggcgcgcg tattcaccgc ccctggtgcg
    27481 caaggcatca agttcggcca gcgtggcgtt gtcaatacga taaacttcac cgtgtactgt
    27541 tccgttcccc ggaactgcgc ctggatagtg gcccaggcta tacaactggt agttatcgat
    27601 actgaaatcg cccagtaact gggcattggt catccagtga ctgttgcctt gtttgtggcg
    27661 taaactgccg tagacaaata ttcgcattgc taaaactcga actgatagag caaatccagt
    27721 gcctggtcta caccagacac ggcttccaga tatagcttag gcatcaggcg ataacgtaac
    27781 gtgagtgttg ctatagagtc aaatataccc acgccgtatt tcacttgcag acctggcaat
    27841 acatagccgc tgaccactac ctgggaggag tcgcctactc cctgggtgtc gagcgctaaa
    27901 ttgcttacgc caaacgtctc gccgatttta cccacaatct ggccactttg cgcaaccccc
    27961 agaccaatca gcatcgaggt cattgccgca ctgtcgctct gatcgctctc cagcccttgt
    28021 ccacgtagca aataagacaa tgcagcttgt tgcgacatcg ccgggtcaga gaagatctcc
    28081 gctttcggtt cgtccgccag accagtgacg cgaactccgg cgattacgtc gtcttctgta
    28141 gcatccgggt tacgaatagc ttcaatatta agatacggtt gatccggcgg accagagaac
    28201 agtaactcac ctttacgcac aatcagatcc tgaccatagg catggaagcg cccttcaggg
    28261 atgttgatct gcccgttcag gcccagccct tgtttgtcct gaacgacgtt aagatcgccc
    28321 gtcagccgcg ctttcaggcc aaaggcgtca atgcgcacat tgttgccaac gtggacaatc
    28381 aggttactgt taatcggaat cgacgccgtt ttcggctctt ccggttgcag gttatcgtta
    28441 agcatcacca catcgctgga gacgcctact gcgctttccg gcagatcgtg caccacgatg
    28501 cgcgcccacg ggacatccac gcgaccatcg agggtaaaca ggtttggtgt agcctcgaag
    28561 acaacatctg gcgatacatc cattcgtacc atcggcggca cggtgatccg cactttactg
    28621 cctttcgccg ttacccgcgc ccgccagttt tcaatttggc tccagtcggc atcaccgttc
    28681 aggtagattt caccctgctg ggtccgtact gtaccggcaa gcgtcgagcg cataccatta
    28741 aagttgaccg caagctggct cggttgcata tcaaacggca taaagttgcc gtcgatatcc
    28801 acacccgtaa cctgaagctg accaaacaac tgcgggcttt gcacatcacc acccagacgc
    28861 aagttggcac tcaccatccc cgctgctttt tccccacggg taaagatggg gtttatcatc
    28921 gcaaggttga agttacggat attgacgttg ccaccaagat tacggcggcc ttgcggatcg
    28981 gtcacctgca cctgtccatc aaactggccg ttattggtca ggcggatggt ccagcccaat
    29041 tcggcacggt tgttacgcaa ttccgccgtc agattcagtg tctgaaacgc cacgggcagc
    29101 gccgcatcgt tgacggtttg cgttacctgc acgttacgcc ccgaaagggt gatactgccc
    29161 tgcggcagcc cctctttggt ggtgtcccag gcaacgtccg ctttacccgt gaagataccg
    29221 ctggcctgag tggtttctgg cataaatggt ttcagcatgg ccaggtcgaa gcggttgaga
    29281 ttcaccaccg cacgcccttc ggcacccgca tcgatagttt gcggcacgca cagttccgca
    29341 ttcgggttaa gccagcagtg tggcccgatg ctgatttttt gctccttatt gcggtaatcc
    29401 agcgcaatat cgcgggtcag cgaccacggg ccgaccggcg tctggaagcg ggtattgcta
    29461 agagttccct tccagcgttc ttctttgcga tcaaaacttc ctgccagatt aagctgcccg
    29521 gagacaggct cgccctgaat ccgcaactgt aactcgtgct gtttttcgct gcctttggca
    29581 ttcagggtga cgaggttgat atttacatcc ggctgcgaaa tttgctcaac gcgtacgtcg
    29641 agtttcccgg cgatctgatc ggtggattta atgtcgcctt ccacgcgaac ctgcgccacg
    29701 gaaagttcct gccagcgcag gccgcgcgcg gtgatatctg ccagtagttg tggcgcttcc
    29761 accgtgccgc gtactttcac cagccctttc gctgtaccgc caagccccgg cagcgcgtta
    29821 tccagccccg gcgcgttgat ggtggcatca agattgagat cttttacccc cagctcgcct
    29881 ttcacttcgg cactgtttgg ccccagttcc agatgaagcc ctgggatcat ccactgcata
    29941 taactgttgc ctttcagcgt gccgtcaacg ttcactttgt tctgtttgac gttaccggtc
    30001 agcttcagtt ctggcacctc catctgccag gtgccgccgt acaggctacc gcgggtttta
    30061 atcaagccat tgagtttcga cggccactct gggatctctt tggcggtgtt aatgccgtta
    30121 agcgttagct caccgcgcca actaatggcc tgctgccagt cgagcaacgc cttgagttca
    30181 gttttccctt ccagcgccgc gacggtgagt ttgtcgagat tgacctgctg ttcattacct
    30241 ttggcatcaa gggtaatggt ggctggcggg atctccagtc ccttcactgc cgtacgcata
    30301 gagagcgtgt aatcggtcat tttgccggta agtttcagtt tcagatcatc cgcctgatac
    30361 tgcttctcac cagtgaacgg ccagtaaatc tgtttgctgt tcacttccac gttgagcggc
    30421 aatccggctt ccgccagtcg cgtctgggcg cgtaaatcca tatccaccgg accggaaaga
    30481 ttaacgccaa tctccagctg ttcgcgcagc gcgccgccca ctttcagctt caccttttca
    30541 cctttcaacg gctccacgtt cagtgtacta ttgagagtga tgtccaccgg ccagttgtct
    30601 gacagctgcg ccgtgccgct ggcgttgacg atcccttgac tggaatcgat atccagggcg
    30661 tccagtttag tattgccgtc aatgctgctc actttcagca gcatggtgct cacggtgatg
    30721 tccgtgtcgc ccgtcacgcg caactgctcg cccttaaact cttcaatgtt caggttaagc
    30781 ggcaaatgca cgtcggtcat ttccggcaat accgggcgag aaaagagatc tttcagcgtt
    30841 tcgccgagcg gcttttcctc cggctgcgga ttttcaattt tcggttcgac cacttcttcc
    30901 tgcgccactt ccgccacttt cggcagagca atcagcaggc ctttcagcga cgtcggtttc
    30961 agggtcaggg ttttctcctg ccagttcagg ccggaggtga agtccatcac cgataccgtg
    31021 gtgtcatcaa tcttgatgtt gacgttgtcc agtgccaccc gtgttagggt gatgggatac
    31081 ggcgtggaga gatccagcgg accgctatct tcctcttctt caacctgttc agaagggggc
    31141 atttttttgc tgtcgatatt gacctggatg tctttcagcg ccagatcatt aatacaaaca
    31201 ctgctgttcc acaggcactc aagcccgacc gccagatgca gattgcctgc ttttaccgcc
    31261 acgcctggct gctcataacg aacgtcagac aaggtgagat cgcgccagcc gccggtgacc
    31321 ttgccaacat ccagtcctgg cacccagcga tccgccgctt taaataccag atgcaggccg
    31381 ctggtggtgc ccaccagaaa cgccaccgat cccagcaaca gtaagataac gataaccacg
    31441 ccgaggctga tttttttcca taaactcata attctggccc cagaccgatg taaaactgta
    31501 acccgtgttc gtctttatcc gcgaccggta cggcaaaatc gagtttgatt ggcccgaccg
    31561 gcgattccca gcgtacgccg accccggtac cggttttaaa gtcgctgcgg cgaatatcgc
    31621 ttaccgcttc gccactatcg acaaacaccg cgccccacca ttttccggtc acgttgtact
    31681 ggtactccag cgatccggtt atcaacttcg aggccccttt caggtcaccg ttggcgtatt
    31741 tcggagcgat agatttgtat ttgtagccac gaatactgcg gtcgcccccg gcgaaaaaac
    31801 gcagatccgg cggtactttg tcgaaatcac cggtttcaat ccagcccagc gtgccgcgtg
    31861 tcacaaaacg atggcgatcg tacagtgtgc ggatccagac gttttgtgcc tggaaaacgg
    31921 agaaatcgac atctgagccc caggcagtgt tggagtagtc gatagagtag cgttgcgagt
    31981 cgccccaggt tggcatcagg ccaccacgag aacgcgtgcg gctaatcatc accccaggat
    32041 aaaacagcat cgtggtgttg gtaatttcac cctgggtaaa gtggtcgaga ctccagcgca
    32101 ggttaatggc acgctgccag ccgctggaga gatcccagta gcgagaagcc accagcgtag
    32161 tggagtcaga ttcggtatcg ttcaggtcag tgcgcttaaa accgccctgc accaaataat
    32221 attgttccag tggattcttc agcagcggca ttttatagct gaagtcgagg atctgttccg
    32281 gcgcggaaat actggtactg gtggtcagac tgtgaccgta tgagttcatc cacggttttt
    32341 tccacgtcgc tttcacgcgc ggtcccacgt ccgtagagta accgaccccg gtttcgatgg
    32401 tgttttctgt tcgcggcgaa accacgcccg tcaatggtaa tactttcgtt tcgcgcgctt
    32461 tatcaaattg tggagccacc accaccgagt taaaccagcc ggtagcagaa agtcggcggt
    32521 tcagttccgc cagatctttc gattcgtact catcgccctc tttaaacggc accagatttt
    32581 gcaggtattc atcgcggatt tgtgatcctt caaaggtcac atgcccaaag cggtaacgtt
    32641 cgccactgtt ataatcaata tcccagaagg ctttatgcag gccgagcgca atgcccagct
    32701 gcgctttggt aaattcgcta tcgaaataac ctttacgcaa cgcaatgctg gttaaggact
    32761 ttttgaaatt ttcataatcg ccctggttca gcaccgtgcc aatagccggg cgagtatcga
    32821 gcaatttcaa atagtcttta tcggtccgcg cgccgccgcg caataccaca tcggtgccgc
    32881 caattaacac cggcacgcct ggcgtgactt tggcgatcaa tacctgccgc cctttctttg
    32941 gcggtggacg gagatcaaat tcaatggtcg gctggtaata acccagcgct ttcagacctt
    33001 cgcggatggc atcatcgacg cgtgcgcgaa agcgacggtc tggcgtcact tcatcacttt
    33061 caatcgtaga aagctgcgca cgaacgttct tttccagctg tcccgataac ccctcgacct
    33121 gtagacggac gttcgcggcg acggcagatc cgcttaagca gagtaagctt acacagcata
    33181 actgtcggat atagcgcaca ttttctcctg aatatccttt tttcctgccc ctggaacgcc
    33241 gttaaacggc ttaacaaaaa tccagtaata tggattaaaa agcagactaa accccaaata
    33301 tttcttatgt tttactttag acctattcac ggtggttatt gtgtgcaaat acgcctcttg
    33361 ttacaacctt aaccccaatg accgattttc gggagagcga caccatgagt ttatttgata
    33421 aaaagcatct ggtttccccc gccgatgccc tgcctggacg taacaccccg atgcccgtag
    33481 ccacgctgca tgcggtcaac ggtcactcaa tgaccaatgt acctgacgga atggagattg
    33541 ccatttttgc gatgggttgt ttctggggtg tggagcgtct gttctggcag ttacccggcg
    33601 tttacagcac cgccgcaggc tataccggag gctatacgcc aaatccgact tatcgggaag
    33661 tgtgctccgg tgatacgggt catgccgaag cggtacgcat tgtttacgat ccttccgtca
    33721 tcagctatga gcagttgcta caggtatttt gggagaatca cgatcccgcc cagggaatgc
    33781 gtcagggcaa tgaccacggc acgcagtatc gttcagcgat ttatccgctg accccagaac
    33841 aggatgccgc agctcgcgcc agtctggaac gttttcaggc ggcgatgctt gccgccgatg
    33901 atgatcgtca catcaccacg gaaatcgcta acgccacacc gttttattat gccgaagatg
    33961 accaccagca atatctgcat aaaaacccgt atggttactg tggaattggc ggaattggcg
    34021 tctgtctgcc accggaagca tagcgttacg ggtacaaatg tagattgttg ataaagtgcg
    34081 ctttatttat gccagatgcg gcgtaaacgc cttaccaggc ctacaaaatc gtccaaattc
    34141 aatatattgc aaggactgcg taggcctgat tggcatagcg catcaggcag tattgcattt
    34201 atcagcagtc tgaattttaa ccctctggcg actttacagt accttacgct atactagcca
    34261 ctgaaaatgc cggttcactt tcttcgaatc ggctttcaat gtgtatttca cacaaattaa
    34321 tcaacttccc cttccgagga tctggcctga aaggtcggat aagatatgtt aaacagtatt
    34381 ttagtcatac tctgcttgat cgctgtaagt gcgttcttct cgatgtccga gatctcactt
    34441 gccgcctcac gcaaaatcaa acttaaactg ctggctgatg aaggcaatat aaatgcccaa
    34501 cgcgttctga atatgcagga aaatcccggc atgttcttta ccgtggtcca aatcggtctg
    34561 aacgcagtgg cgattctcgg cggtatcgtc ggtgatgcgg cattttctcc agcttttcac
    34621 agcctgttct cccgctatat gtcggcagag ctctctgagc aactgagctt tattctctct
    34681 ttctcgttag tgactggcat gtttatcctg tttgcggatt taaccccgaa acgcatcggt
    34741 atgattgcgc cagaagctgt cgctttgcgt atcatcaacc cgatgcgctt ctgcctgtac
    34801 gtttgcaccc cgctggtgtg gttcttcaac ggactggcga acataatctt ccgtattttc
    34861 aaactgccaa tggtacgtaa agatgacatc acttctgatg acatctacgc ggtagtggaa
    34921 gccggtgcgc tggcgggcgt gttacgtaaa caggaacacg agctgattga aaacgtcttt
    34981 gagctggaat cccgtaccgt tccgtcgtca atgacaccgc gtgaaaacgt gatttggttt
    35041 gatctccacg aagatgagca aagcctgaag aataaggtgg cggaacatcc gcactctaag
    35101 ttcctcgtct gtaatgaaga tattgaccac atcatcggct atgtcgattc taaagacctg
    35161 ctgaaccgcg tgctggctaa ccaaagcctg gcactgaaca gcggcgtaca aattcgcaac
    35221 acgctgattg tgccggatac gttaaccctt tcagaggcgt tggaaagttt taaaaccgca
    35281 ggtgaagact tcgcggtgat catgaacgag tacgcgctgg tggtggggat catcaccctc
    35341 aatgacgtga tgaccacgct gatgggcgat ctagtcggtc aggggctgga agagcagatt
    35401 gtcgcccgtg atgagaactc atggctgatt gacggcggca ccccaattga cgacgtcatg
    35461 cgcgtgctgg atattgacga gttcccgcag tcgggcaact acgaaaccat cggcggcttt
    35521 atgatgttta tgctgcgtaa gatcccgaaa cgcaccgatt cggtgaaatt cgccggctac
    35581 aaatttgaag tggtggatat cgataactac cgcatcgacc agctgctggt gacccggatc
    35641 gacagcaagg ccaccgccct ttcgccaaaa ctgcctgacg ctaaagataa agaagaaagc
    35701 gtcgcgtaac ccccagaaac atcaacggct cctgaatcag gagccgtctt attactgcat
    35761 agcactttgg ttaagccatc tctgtttgca gacgcataac ctgacggtta acttcggaca
    35821 tcactgacaa atgcagctta tccttcactt ttgggataag aatcttaccc ttatcaaatt
    35881 caaaagcgcc aacgtccttg atgtataacc gtccacggaa caggatcttt acgtacttcg
    35941 ccacctgaag tgggttgtac cgttggaaaa ttttcattgt tatctcctgc tgagtattac
    36001 gcctgtgcgg tgccacaatc ggcccaacta ttatgaggcg caaattttaa tgcttagtga
    36061 ctatagacta tccgggcaat gtttccaccg cgtataactt tttttaccta aaggttacaa
    36121 ttattcagaa ttatcttttt accgaagcgc ggtcttcagt ataagcattc atttttcata
    36181 tgatttgtgc gcttgaccgc aaactggcat cacacttgcg ggaaattcga taaatagcac
    36241 atatgattaa aactcagacc caagtggtcg gatcacctgc atatcataag aaggaaacac
    36301 catgacccta cgcaagattc tggcactcac ctgcctgctg ttgccgatga tggcttccgc
    36361 acatcagttc gaaaccggtc agcgagtgcc gccgattggc attaccgatc gaggcgagtt
    36421 ggtgcttgat aaagatcagt ttagctacaa aacctggaac agcgcacagt tagtgggaaa
    36481 agtgcgagta ctgcaacata ttgctggtcg cacctctgca aaagagaaaa acgcgacgct
    36541 gattgaagcg attaaatcag cgaagttacc gcacgatcgc taccagacca ccaccattgt
    36601 taacaccgac gacgcgattc cgggttcagg gatgtttgtg cgcagcagtc tggagagtaa
    36661 taaaaagctt tatccctggt cgcagtttat tgtcgatagc aatggcgtcg cgcgcggtgc
    36721 ctggcagctg gatgaagaaa gttccgctgt agtggtgctg gataaggatg gtcgcgtgca
    36781 atgggccaaa gacggggcgc tcacacagga agaggtgcag caagtgatgg acctgctgca
    36841 taaattaatt aataaataga cactctgaat cccggattca ggaacgactc gcgcggagtg
    36901 taatccagcg gtttaccctg ccagtcatga acgtgcgctc cggcagctgc agccacagca
    36961 tgaccagcgg cggtatccca aatattcgtt ggtccgaagc gcgggtacag ctgcgcctgc
    37021 ccttccgcca ccaggcagaa tttcagcgaa gagccgatgg acgtggtctg atgttcgcca
    37081 agctgttgca gatactcttt cagctccgca tctgcatggg aacggctgat aaccaccagc
    37141 ggcgggcgcg catcgcggac ctgaatctgc ttgcgcacac cgcactcttc tttccaggcc
    37201 ttgccttctg ccgcgctgta cattatgttc attaccggcg catacaccac acctaaaatc
    37261 ggtttgccat ggtcaatgag cgcaatgtta acggtgaatt cgccattacg tttaataaac
    37321 tctttagtac catccagcgg gtctaccagc cagtaacgct gccagtgctg acggacttcc
    37381 caaccgggag gatcttcttc agaaaggacc ggaatatccg gtgtcagcgt acgtaaaccg
    37441 tccatgataa cggtgtgagc ggcaatatcc gctgccgtta ccggagaatt gtccgctttg
    37501 ctgacgacgt ccatcggttt cgtcccgtcg tagacctgca taatggcatc gcctgcattc
    37561 cgtgcaagct ggcatacttg atctaacatt tctccacctc gtctctgtga gcggtgttaa
    37621 cttattgttt tacttatacc ctatcgttaa tgaatgcgcc aactgtgata gtgtcatcat
    37681 tttcaaagcg taaaattgtg gcattcttca ctgttctata agtaaggcgt ttattctccc
    37741 ttttctttcg tattcccgat gataaaagga tgtccctgat gattaagttt agcgcaacgc
    37801 tcctggccac gctgattgcc gccagtgtga atgcagcgac ggtcgatcta cgtatcatgg
    37861 aaaccactga tctgcatagc aacatgatgg atttcgatta ttacaaagac accgccacgg
    37921 aaaaattcgg actggtacgt acggcaagcc tgattaacga tgcccgcaat gaagtgaaaa
    37981 acagcgtact ggtcgataac ggcgatttga ttcaggggag tccgctggcc gattacatat
    38041 cggcgaaagg attaaaagca ggtgatgttc atccggttta taaggcgctg aatacgctgg
    38101 attatacggt cggtacactc ggcaatcatg aatttaacta cggtctggat tacctgaaaa
    38161 atgcgttggc gggagcgaaa ttcccttatg taaatgccaa cgtcattgac gccagaacca
    38221 aacagccaat gtttacaccg tatttaatta aagataccga agtggtcgat aaagacggaa
    38281 aaaaacagac gctgaagatt ggctatattg gcgtcgtgcc gccgcaaatc atgggctggg
    38341 ataaagctaa tttatccgga aaagtgacgg tgaatgatat taccgaaacc gtgcgcaaat
    38401 acgtgcctga aatgcgcgag aaaggtgccg atgtcgttgt cgttctggcg cattccgggc
    38461 tatctgccga tccgtataaa gtgatggcgg aaaactcagt ttattacctc agtgaaattc
    38521 ctggcgttaa cgccattatg tttggccatg ctcacgccgt tttcccaggt aaagattttg
    38581 ctgatatcga aggggctgat atcgcgaaag gcacgctgaa tggtgttccg gcggtaatgc
    38641 cgggcatgtg gggcgatcat cttggggtgg tcgacttaca actcagtaat aacagcggta
    38701 aatggcaggt gacgcaggcg aaagcggaag cacggccgat ttacgacatc gccaataaaa
    38761 aatccctcgc ggcggaagac agcaagctgg tagaaacact caaagccgat cacgatgcca
    38821 cacgccagtt cgtcagcaag ccaatcggta aatccgccga caatatgtat agctatctgg
    38881 cgctggtgca ggacgatccg accgtgcagg tggtgaacaa cgcgcaaaaa gcgtatgtcg
    38941 agcattacat tcagggcgat ccggatctgg caaaactgcc ggtgctttca gctgctgcac
    39001 cgtttaaagt tggtggtcgc aaaaatgatc cggcaagcta tgtggaggtg gaaaaaggcc
    39061 agttgacctt ccgtaatgcc gccgatcttt atctctaccc caatacgctg attgtggtga
    39121 aagccagcgg taaagaggtg aaagagtggc tggagtgctc cgccggacag tttaaccaga
    39181 ttgatcctga caacacgaaa ccacagtcac tcatcaactg ggatggtttc cgcacctata
    39241 actttgatgt gattgatggt gtgaattatc agattgatgt tacccagcct gcccgttatg
    39301 acggcgagtg ccagatggtt aatgccaatg cggaaaggat taagaacctg acctttaatg
    39361 gcaagccgat tgatccgaac gccatgttcc tggttgccac caataactat cgcgcttacg
    39421 gcggcaaatt tgccggtacg ggcgacagcc atatcgcttt tgcttcaccg gatgagaacc
    39481 gctcggtgct ggcagcgtgg attgctgatg agtcgaaacg tgcgggggaa attcacccgg
    39541 cggcagataa caactggcgt ttagcaccga tagctggcga taagaaactg gatatccgtt
    39601 tcgaaacctc tccatcagat aaagccgcgg cgtttattaa agagaaaggg caatatccga
    39661 tgaataaagt cgcgaccgat gatatcgggt ttgcgattta tcaggtggat ttgagtaagt
    39721 aatacacttc tttttcggcc tataaatcat caaccgcatc cggcactttt gtcggatgcg
    39781 atgctggcgc atcttatccg ccctacaagt catgaaccgt aggccggata agcgcagccg
    39841 gcagttatgc cgcacgttca tcccgcaccg ccaacacctc gggcaaattc aactcaatcc
    39901 agtccgccag tgcagcaacc ttttcgctca cctgctcgcc cagcggcgtg aggctatatt
    39961 ccacatgcgg cggcaccacc ggatacgcga tacggttaag aaaaccatcc tgttctaacg
    40021 cctgtaacga ctgcgcaagc atcttttcac tcaccccacc gattttgcgc cgcaggtcgc
    40081 taaagcgatg agtaccttcg cgtagcgcca ccagaatcaa caccccccaa cggctggtga
    40141 cgtgtttcaa cacctcgcgc gacgggcact gttccgcaaa gagattaccc tctttcagtt
    40201 gttgtgacag gctaacctga ctcatttcat acttaccttt ttgtacgtac ttactaaaag
    40261 taagtttagg tgttagcgta tttaaacaca agacaaaacg atggagactt cccatgatcg
    40321 ctattactgg tgccactggc caacttggtc actatgttat taaatccttg atgaaaacgg
    40381 ttcctgccag ccaaatagtg gctatcgttc gtaatccggc aaaagcccag gcactgacag
    40441 cacaaggcat taccgtgcgt caggctgact acggcgatga agccgcactg acatctgcac
    40501 ttcagggagt ggaaaaacta ctgctgatct cttccagcga agtgggtcaa cgtgccccgc
    40561 agcatcgtaa tgttattaat gccgcaaaga cggctggcgt gaaatttatc gcttatacca
    40621 gtctgcttca cgcggataaa tctccgctcg gcctcgccga tgagcacatc gagacggaga
    40681 aaatgttggc tgattctggc atcgtttaca ccctgctgcg caacggctgg tacaccgaaa
    40741 actatctcgc cagcgccccg gcagcactgg agcacggcgt atttatcggt gccgcgggcg
    40801 atggcaaaat tgcctcggca acgcgggcag attatgcggc agctgcggca cgcgtgatta
    40861 gcgaagccgg tcacgaaggc aaggtttacg agctggcggg cgatagtgcc tggacgttga
    40921 cacagttagc ggcagagctg accaaacaga gcggcaaaca ggttacctat caaaatctga
    40981 gcgaaaccga tttcgctgcg gcgctgaaaa gcgttggcct gcctgacgga ctggcggata
    41041 tgctggcgga ttctgacgtt ggcgcatcga aaggcggtct gtttgatgac agcaaaacgc
    41101 tgagcaaatt gattggccgc ccaacgacaa cgttagccga aagcgtaagc catcttttta
    41161 atgttaataa atagttaatt aaagtggcat cctcccgcat cctctctgat aatgacggga
    41221 tgccgggagc aatcatgtct gcttcctgaa cgtttcttct gacagaccaa tggatgccag
    41281 taatgattaa cggcgtgctg tacgccctgt tagcagggtt gatgtggggg cttatttttg
    41341 tcgggccgtt gatcgtgccg gaatacccgg cgatgttgca gtcgatgggg cgttatctgg
    41401 cgttagggtt aattgcgcta cccattgcct ggctgggacg cgtgcgtctg cgtcagttgg
    41461 cgcgtcgcga ctggcttacc gccttgatgc tcactatgat gggcaacctc atctattact
    41521 tctgccttgc cagtgccatt caacgtactg gcgcgcctgt ttctacgatg attattggca
    41581 ccctgccggt ggtcattcct gtctttgcca atctgcttta tagccagcgc gacggcaaac
    41641 tcgcgtgggg aaaactcgcc ccggcactga tttgtattgg catcggcctg gcgtgtgtga
    41701 atattgctga gttaaaccac ggactccccg attttgactg ggcacgttat acctctggca
    41761 tcgtgctagc gttagtttcc gtggtctgct gggcatggta tgccctgcgc aacgcccgct
    41821 ggctgcggga aaatcccgac aaacatccga tgatgtgggc gacggcgcag gcgctggtca
    41881 cgctgccggt ttctctcatc ggctatctcg tcgcctgtta ctggctgaat atacaaacgc
    41941 cggacttctc cttacctttt ggcccccgtc cgctggtgtt tattagtctg atggttgcga
    42001 tagccgtgct ttgctcatgg gttggcgcac tctgctggaa cgtcgccagc cagcgattac
    42061 cgacagtgat tctcgggccg ctgatcgttt tcgaaacact ggcaggtttg ctgtacacct
    42121 ttttgatacg ccagcaaatg ccgccgctga tgacgctgag cggtatcgcg ctgttagtgg
    42181 ttggcgtggt cattgcagtc agagcaaaac cggaaaagcc tttaactgaa tctgtctcag
    42241 aaagttgaca tactggcagt gagttaaata agcctctgct acgtaagggt tatagcattt
    42301 gccttaaaga tgcatttaaa atacatctta tcttattaag aatgaggtat cagctatggc
    42361 ttatcgcgac caacctttag gtgaactggc gctctctatc cctcgcgctt cggctctgtt
    42421 tcgtaaatat gatatggatt actgctgcgg cggtaagcaa acgctggcgc gtgcggcggc
    42481 acgtaaagaa ctggatgttg aggtcattga agctgagctg gcaaagcttg ctgaacaacc
    42541 gattgagaaa gactggcgta gcgccccgct ggcagaaatc atcgaccata tcatcgtgcg
    42601 ctaccacgat cgtcaccgcg agcaactgcc ggagctgatc ctgcaagcga ctaaagtcga
    42661 gcgcgttcac gccgacaaac cgagcgtgcc aaaagggctg acaaaatacc tgaccatgct
    42721 gcatgaagag ctttccagcc acatgatgaa agaagagcag atcctcttcc cgatgatcaa
    42781 acaaggcatg ggcagccagg caatggggcc aatcagcgta atggaaagcg agcacgatga
    42841 agcgggcgaa ctgctggaag tgatcaaaca cactaccaat aacgtcacac cgccgccaga
    42901 agcctgcacc acctggaaag cgatgtataa cggcattaat gaactgattg atgacctgat
    42961 ggatcacatc agtctggaaa acaatgtact gttcccacgc gcgctggcgg gtgagtaatc
    43021 taaaagctgg atggcattgc gccatccagc atgataatgc ggttatttcc gcagttcagc
    43081 agcccgcttc ttaccaataa acaaccagcc cagccccagt gcgataaacc acagcggggt
    43141 gaccaacagc gcctggcgag tgtcatcttc cagtgtcagc aacacgacca cgaacacaaa
    43201 gaacgccata catacccagc acatcagctt gccgagcggc atcttgtaga tcgacttctc
    43261 atgcagatga ggacgctgtt tgcggtaaac caggtacgag caaaggataa tcgtccagac
    43321 gaacataaac agaatcgcgg aaacggttgt aatcatcgtg aacgcgccaa tcacgctagg
    43381 attcacatac agcatcacca cgccaccgag caggcagatg caggagaagg tcagcccttt
    43441 cgctggtact gcgcgcttag aaagtttagc gaacgctttc ggtgccacac cttcctgcgc
    43501 cagaccaaac agcatacggc tggtagagaa gacgccgctg ttagcggaag acgccgcaga
    43561 ggtcagcacc acaaagttga tcacgcttgc ggcagcaggc agccctacca acacgaacag
    43621 ttcaacaaac gggcttttct ccgggactac cgaactccac ggcgtcacgg acataatcac
    43681 aatcagcgcg aagacgtaga acataatgat acggatcgga atggagttaa tcgcgcgtgg
    43741 cagtgatttc tccggatctt tggtttccgc agctgttgta cctaccagct caatccccac
    43801 gaaagcgaaa accgctatct ggaatccggc aaagaagcca cttaaacctt tcgggaacca
    43861 gccgccgtca ttccacaaat gcgcgaatga cgcttccaca ccggtcggtg actgaaagtg
    43921 catcgccacc atgaccaggc cgacgacaat cagcgacacg atggcgacga ttttgatcat
    43981 cgcaaaccag aactccatct caccgaacat tttcacggtg gcaaggttga gcgtcaacag
    44041 cagcactatc accgccagcg aggcgaccca gtcggagaga tcggggaacc agaactgagc
    44101 ataagcagtg atcgccacca cgtctgccat accggttaca acccagcaga accagtaagt
    44161 ccagccggtg aaatatcctg cccacggccc gagtaaatcg gaagcgaagt cactaaaaga
    44221 tttgtattcc agattcgaaa gcagcaattc ccccattgcc cgcatcacga aaaagagcat
    44281 aaaaccgatg atcatataaa cgaaaatgat cgacggcccg gcaaggctaa tcgttttgcc
    44341 agaccccata aacaaccccg taccaatggc accgccaatg gcaataagct gaatatgtcg
    44401 gtttgtgaga ttgcgccgta gcgactgttc agccggagcc tgatcatcgg caacgacttt
    44461 tacctgatct accatgtttt tttcttcctg tacctgtctg tgttgttcag gctctacggc
    44521 ctttagtcag tctatgatac gacgaatctg tatcgggact catcgatatt aggtaacaat
    44581 acgcgggatg aatactaaga tttagatcta atgttaattt tatgtttaaa gtgagtgtca
    44641 tatcacggtg ataacgcgaa acagctcaca aaaatacacg cttacggggt ctttgcaaca
    44701 caaaatagcg ccgtttggca aactgaaggg tttattgctg aatgcctgct cccctctggt
    44761 tcgaggggaa cagaatgcgc tgcttagagg atttccagca gttcgacttc aaacaccagg
    44821 gtgctgaacg gagggatgga tgcgcctgcg ccgcgctcgc catatgccag ttcctgcggg
    44881 atagtcagtt cccatttaga acctaccggc atcagagtca gtgcttcaat ccagccaggg
    44941 atcacgccat taaccgggaa ttcagcgggt tcaccacgag caacggagct gtcaaacacg
    45001 gtgccgtcga tcagtttacc ggtgtaatga acacgaacgc ggtcagtacg tgccggaatt
    45061 gcgccttcac cctggttgat cacgcggaat tgcaggccag attcggtgct gttcacacct
    45121 tcttttttgg cattttcttc aagatatttc acaccttcgg cagccatcgc ctggaaacgc
    45181 tgacgacgaa cggcatcggc gcgttcgtgg atttcacgca gcgcgcgatg caccacatca
    45241 accggaacag ccggatgttt gccttccagc gcatctgcaa taccagccac cagcgcctcc
    45301 ggcagcagcc cttgcaggcc agattcactc agttgttggc ctacctgtaa accaatgccg
    45361 tagcttgctt gcgcttcgat ggtgtcaaaa gttggggtgg tcatcgtatt tcctttctta
    45421 gggtataaaa gtagcgggca gcatatcagc cacacttctt tgggtaaaac tttgtcgcag
    45481 gaatgatgac aaatcccggg gaaaaagaaa caatagcaat atcccgtatg aatataataa
    45541 aggcgggttt accacggaca tcatagcgtt agcttattca taggctatga ttgaggaaca
    45601 agacgcggag caggaggaaa ccatgcccgg gcgctttgaa ctaaaaccaa ccctggagaa
    45661 agtctggcac gcgccggaca attttcgctt catggacccg ctgccgccga tgcatcgtcg
    45721 ggggatcatt attgccgcca ttatattggt ggtcggattt ctgcttccat ctgatgatac
    45781 gcccaacgcg ccggtggtga cgcgtgaagc gcagttagat attcagtcgc aatcacaacc
    45841 gccgacagaa gaacagctgc gggcgcaatt agtcacacca caaaatgatc ctgatcaggt
    45901 ggctccggtc gctccagaac ctatacagga aggccagcca gaagaacagc cgcaaactac
    45961 ccaaacacaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    46021 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    46081 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaacgggca
    46141 aatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    46201 gaataatcaa caggtcctgt tcacccgcca gccggatggc agttttattc gtgcgcggta
    46261 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    46321 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    46381 atcaaataat atccgccaag cgcagcaata ttaatattca cctgcaatgg gtcgatatct
    46441 ggcttaaaaa tattgtgttt cttcccttca tccaataagg attccattaa ctgcaagtgc
    46501 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    46561 tcactatgca caatttttaa aaaccaggga ttagcaatat aataatccca tgtaaaatca
    46621 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    46681 tgtcgaatat cgttccaggt atattccagt acttcaataa ataaattttc tttattacca
    46741 aagtgatgat aaaccgtctg tttactacac cccgcagcct taacaatatt atcgaccctg
    46801 gccccttcat aaccatattc ggcaaattca ttaaccgcgc ataacagcag cttttccttc
    46861 aggctctgta cgttgctggt atcaagatac ataacgcttc cccacgacct cttcaagata
    46921 actatcttaa cccgattaat tagaagctcc agtaaaaagg ataaaaactt gtactttcat
    46981 caagttatca aatgtcctgt gatcactatc gcatatcaac caaccagatg gttgatcttt
    47041 cttgcgtcac gtcgcaagct aattgtcact tagaaaacaa tattcaaaaa aagagagcta
    47101 ttgtatgaaa tccactcgtc ctgttgccgt gattaccgga gccgctcgtg gtattggaaa
    47161 gggctgtgcg ctggagcttg ctcgtggcgg atttaacctg ctgattaacg atcttcccga
    47221 tgccgacagc gtagaaaaac tgcatatcac acaacaagaa tgtatagctg aaggcgtgga
    47281 agtgatttgc ttccctgccg atgtcggcga tctctccttg catgaagaga tgctcgacgc
    47341 ggcgcaaaac cagtgggggc gtctggactg cctgctcaat aacgcgggaa tttcagtgaa
    47401 aaaacgcggt gacctcctcg acctcgaacc agacagcttc gatcaaaaca ttgccattaa
    47461 cacccgcgcg ccgttcttcc ttgctcaggc attcagtaag cgtttactgg cacagccgaa
    47521 accggaagcg gaactgccgc accgctcgat tattttcgtc agttccatca acgccatcat
    47581 gctggcgatg aatcgcggcg aatacaccat tgccaaaacc gccgtttctg ccgctgctcg
    47641 tctgtttgct gctcgccttt gcaatgaaca aattggcgtt tatgaagtgc gccctggtct
    47701 catcaaaacc gatatgacta ttccggcaac ggcttattac gacgaactga ttgccaaagg
    47761 tctggtgccg tggggacgtt ggggttatcc ggctgatatc gcttccacca tccgcgcgat
    47821 ggcggaaggt aaactgattt acacctgcgg tcaggcggtt gccatcgacg gcggcttaag
    47881 tatgccgcgc ttctgaggct gaatcatgag agatctgtta caacgcccgg atttattcag
    47941 tatcaatacc gcaacactgg gctataaaac gccgctaccc gccattattg atgcctgcgc
    48001 cgcacgcggt attggcgcaa ttgccccctg gcgcagggag ttgcagggtg aagatctcca
    48061 gcaaatcgcc cgccagcttg ccgccagtaa catgagcgtt tccggtttgt gccgtagcac
    48121 ttactacacc gcgccgacac tggcggaacg caaactggcg attgatgata accgccgggc
    48181 gctggacgac gcagcggtgc ttaacgccgc ctgttatatg caggtggttg gcgggttacc
    48241 gcagggaaca aaagatttgt acgaagcgcg ggaacaagta aaacaaggga ttcgccagtt
    48301 gctgccacat tcgaaagatg tcggcgtgcc aattgcgctg gagccgctgc acccgatgac
    48361 tgctgccgac cgctcttgcc tgtgcacgtt acgtcaggcg ctggactggt gtgatgaact
    48421 cgatcccgat ggtgagtttg gcctcggcgt ggcggtggat gtttatcacg tctggtggga
    48481 tccggatctc gccagtcaga tcctgcgtgc cggaaaccgc atcctcgcgt ttcatgtttc
    48541 cgactggtta gtgccaacca ccgatctggt caatgaccgg ggaatgccgg gagatggcgt
    48601 catcaatatt ccgtcaattc gtcggctggt tgaaaacgcc ggatttaatg gcgctattga
    48661 actggaaata ttctcgccgt actggtggca gaaagatatt aacagcacgc tggatattag
    48721 cgtcgatcgc atcgcgcatt attgttaagg aatgatcatg agtgaccaac ccgttctgtt
    48781 cagcagagcg gcagcttcct gccgcctgac attgaaccgt gaagataaat gtcatgctat
    48841 taacgaagag atgattgaat ctctcgacca ttatttaaat gaaattgaaa acgacaccac
    48901 attacgatta gtcgaattaa cggcaaccgg cgataaattc ttttgtgcgg gcggcgatat
    48961 taaatcgtgg tcggcctatt cgccattaga tatgggcaga aaatggatta aacgcggtaa
    49021 tgacgttttt aatcgcctgc gcaacttacc ccaattaacc gttgcgaatc tcaacggtca
    49081 taccatcggc ggtggtattg aacttgcctt gtgctgcgat attcgtattg ctcgccccgg
    49141 cgcgaaattc tctaacccgg aagtgatgct cggcatggtg cctg
//
LOCUS       DAJOSY010000019        48639 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJOSY010000019 DAJOSY010000000
VERSION     DAJOSY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.019"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(74..1933)
                     /gene="gss"
                     /locus_tag="OHN27_001214"
     CDS             complement(74..1933)
                     /gene="gss"
                     /locus_tag="OHN27_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCQ2285696.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDDKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2138..3004
                     /gene="yghU"
                     /locus_tag="OHN27_001215"
     CDS             2138..3004
                     /gene="yghU"
                     /locus_tag="OHN27_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCQ2285697.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3127..3375)
                     /gene="hybG"
                     /locus_tag="OHN27_001216"
     CDS             complement(3127..3375)
                     /gene="hybG"
                     /locus_tag="OHN27_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCQ2285698.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3388..3729)
                     /gene="hypA"
                     /locus_tag="OHN27_001217"
     CDS             complement(3388..3729)
                     /gene="hypA"
                     /locus_tag="OHN27_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCQ2285699.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3722..4210)
                     /gene="hybE"
                     /locus_tag="OHN27_001218"
     CDS             complement(3722..4210)
                     /gene="hybE"
                     /locus_tag="OHN27_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCQ2285700.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4203..4697)
                     /locus_tag="OHN27_001219"
     CDS             complement(4203..4697)
                     /locus_tag="OHN27_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCQ2285701.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4697..6400)
                     /gene="hybC"
                     /locus_tag="OHN27_001220"
     CDS             complement(4697..6400)
                     /gene="hybC"
                     /locus_tag="OHN27_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCQ2285702.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLERG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHIVDADGNEVVSVKV
                     L"
     gene            complement(6397..7575)
                     /gene="hybB"
                     /locus_tag="OHN27_001221"
     CDS             complement(6397..7575)
                     /gene="hybB"
                     /locus_tag="OHN27_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCQ2285703.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICSYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7565..8551)
                     /gene="hybA"
                     /locus_tag="OHN27_001222"
     CDS             complement(7565..8551)
                     /gene="hybA"
                     /locus_tag="OHN27_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCQ2285704.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPQYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8554..9672)
                     /gene="hybO"
                     /locus_tag="OHN27_001223"
     CDS             complement(8554..9672)
                     /gene="hybO"
                     /locus_tag="OHN27_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCQ2285705.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9861..10148)
                     /locus_tag="OHN27_001224"
     CDS             complement(9861..10148)
                     /locus_tag="OHN27_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCQ2285706.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10267..11152)
                     /locus_tag="OHN27_001225"
                     /pseudo
     CDS             complement(10267..11152)
                     /locus_tag="OHN27_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
     gene            11309..12349
                     /gene="gpr"
                     /locus_tag="OHN27_001226"
     CDS             11309..12349
                     /gene="gpr"
                     /locus_tag="OHN27_001226"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCQ2285707.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTKELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12389..12883)
                     /locus_tag="OHN27_001227"
     CDS             complement(12389..12883)
                     /locus_tag="OHN27_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCQ2285708.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13074..13958
                     /gene="yghA"
                     /locus_tag="OHN27_001228"
     CDS             13074..13958
                     /gene="yghA"
                     /locus_tag="OHN27_001228"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCQ2285709.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14230..14655)
                     /gene="exbD"
                     /locus_tag="OHN27_001229"
     CDS             complement(14230..14655)
                     /gene="exbD"
                     /locus_tag="OHN27_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCQ2285710.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14662..15396)
                     /gene="exbB"
                     /locus_tag="OHN27_001230"
     CDS             complement(14662..15396)
                     /gene="exbB"
                     /locus_tag="OHN27_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCQ2285711.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15648..16835
                     /gene="metC"
                     /locus_tag="OHN27_001231"
     CDS             15648..16835
                     /gene="metC"
                     /locus_tag="OHN27_001231"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCQ2285712.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16975..17634
                     /locus_tag="OHN27_001232"
     CDS             16975..17634
                     /locus_tag="OHN27_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2285713.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17674..18573)
                     /gene="yqhC"
                     /locus_tag="OHN27_001233"
     CDS             complement(17674..18573)
                     /gene="yqhC"
                     /locus_tag="OHN27_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCQ2285714.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18767..19930
                     /gene="yqhD"
                     /locus_tag="OHN27_001234"
     CDS             18767..19930
                     /gene="yqhD"
                     /locus_tag="OHN27_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCQ2285715.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20035..20862
                     /gene="dkgA"
                     /locus_tag="OHN27_001235"
     CDS             20035..20862
                     /gene="dkgA"
                     /locus_tag="OHN27_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCQ2285716.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21062..21988
                     /locus_tag="OHN27_001236"
     CDS             21062..21988
                     /locus_tag="OHN27_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCQ2285717.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22039..22296
                     /gene="yqhH"
                     /locus_tag="OHN27_001237"
     CDS             22039..22296
                     /gene="yqhH"
                     /locus_tag="OHN27_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCQ2285718.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22339..24558)
                     /locus_tag="OHN27_001238"
     CDS             complement(22339..24558)
                     /locus_tag="OHN27_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCQ2285719.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24669..26081)
                     /gene="ftsP"
                     /locus_tag="OHN27_001239"
     CDS             complement(24669..26081)
                     /gene="ftsP"
                     /locus_tag="OHN27_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCQ2285720.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMNDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26156..26893)
                     /gene="plsC"
                     /locus_tag="OHN27_001240"
                     /gene_synonym="parF"
     CDS             complement(26156..26893)
                     /gene="plsC"
                     /locus_tag="OHN27_001240"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCQ2285721.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPTDAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27127..29385)
                     /gene="parC"
                     /locus_tag="OHN27_001241"
     CDS             complement(27127..29385)
                     /gene="parC"
                     /locus_tag="OHN27_001241"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCQ2285722.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29523..31130)
                     /locus_tag="OHN27_001242"
     CDS             complement(29523..31130)
                     /locus_tag="OHN27_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2285723.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGKVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31263..31658)
                     /gene="mqsA"
                     /locus_tag="OHN27_001243"
     CDS             complement(31263..31658)
                     /gene="mqsA"
                     /locus_tag="OHN27_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HCQ2285724.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            complement(31660..31956)
                     /gene="mqsR"
                     /locus_tag="OHN27_001244"
     CDS             complement(31660..31956)
                     /gene="mqsR"
                     /locus_tag="OHN27_001244"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HCQ2285725.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            complement(32161..32643)
                     /locus_tag="OHN27_001245"
     CDS             complement(32161..32643)
                     /locus_tag="OHN27_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCQ2285726.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32696..33088)
                     /gene="ygiW"
                     /locus_tag="OHN27_001246"
     CDS             complement(32696..33088)
                     /gene="ygiW"
                     /locus_tag="OHN27_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCQ2285727.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33240..33899
                     /gene="qseB"
                     /locus_tag="OHN27_001247"
     CDS             33240..33899
                     /gene="qseB"
                     /locus_tag="OHN27_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCQ2285728.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33896..35245
                     /gene="qseC"
                     /locus_tag="OHN27_001248"
     CDS             33896..35245
                     /gene="qseC"
                     /locus_tag="OHN27_001248"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCQ2285729.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNRLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(35291..35623)
                     /locus_tag="OHN27_001249"
     CDS             complement(35291..35623)
                     /locus_tag="OHN27_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCQ2285730.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            35942..36523
                     /gene="mdaB"
                     /locus_tag="OHN27_001250"
     CDS             35942..36523
                     /gene="mdaB"
                     /locus_tag="OHN27_001250"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCQ2285731.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            36554..36868
                     /locus_tag="OHN27_001251"
     CDS             36554..36868
                     /locus_tag="OHN27_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCQ2285732.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36916..38808)
                     /gene="parE"
                     /locus_tag="OHN27_001252"
     CDS             complement(36916..38808)
                     /gene="parE"
                     /locus_tag="OHN27_001252"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCQ2285733.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(38837..39418)
                     /gene="yqiA"
                     /locus_tag="OHN27_001253"
     CDS             complement(38837..39418)
                     /gene="yqiA"
                     /locus_tag="OHN27_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCQ2285734.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(39418..40245)
                     /gene="cpdA"
                     /locus_tag="OHN27_001254"
     CDS             complement(39418..40245)
                     /gene="cpdA"
                     /locus_tag="OHN27_001254"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCQ2285735.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(40270..40692)
                     /locus_tag="OHN27_001255"
     CDS             complement(40270..40692)
                     /locus_tag="OHN27_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCQ2285736.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(40693..41322)
                     /gene="nudF"
                     /locus_tag="OHN27_001256"
     CDS             complement(40693..41322)
                     /gene="nudF"
                     /locus_tag="OHN27_001256"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCQ2285737.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            41527..43008
                     /gene="tolC"
                     /locus_tag="OHN27_001257"
     CDS             41527..43008
                     /gene="tolC"
                     /locus_tag="OHN27_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCQ2285738.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            43156..43827
                     /locus_tag="OHN27_001258"
     CDS             43156..43827
                     /locus_tag="OHN27_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCQ2285739.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            43833..44993
                     /locus_tag="OHN27_001259"
     CDS             43833..44993
                     /locus_tag="OHN27_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCQ2285740.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(45031..45819)
                     /gene="ygiD"
                     /locus_tag="OHN27_001260"
     CDS             complement(45031..45819)
                     /gene="ygiD"
                     /locus_tag="OHN27_001260"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCQ2285741.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45962..46735
                     /gene="zupT"
                     /locus_tag="OHN27_001261"
     CDS             45962..46735
                     /gene="zupT"
                     /locus_tag="OHN27_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCQ2285742.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(46793..46963)
                     /locus_tag="OHN27_001262"
     CDS             complement(46793..46963)
                     /locus_tag="OHN27_001262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285743.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(47225..47878)
                     /gene="ribB"
                     /locus_tag="OHN27_001263"
     CDS             complement(47225..47878)
                     /gene="ribB"
                     /locus_tag="OHN27_001263"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCQ2285744.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47982..48130)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            48252..48542
                     /gene="ubiK"
                     /locus_tag="OHN27_001264"
     CDS             48252..48542
                     /gene="ubiK"
                     /locus_tag="OHN27_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCQ2285745.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        12059 a        12946 c        12113 g        11521 t
ORIGIN      
        1 aaatattgaa tattttgatt aatccccgta ctaattattc ttcataatca gtacggggtt
       61 gctacaacat gtattacttt ttcaccacaa ttaacggttc aatatcactc tcttttttga
      121 tgaccagtga ttcatcaccg cgcaaacacg tcccaccgta gttgccgcca acggtgaagg
      181 tacatacctg aatgtattta tcgtccactt tcggcaaaca ccacagttgc tgatagatgt
      241 ttttctgctc ggcaaattta ccgctggttt tgtccagcac ctcttcatga tggctgacca
      301 ggtcgatatt gctaccacag cgaccggcga tcggtttcac tgcgtaacct gttttcacca
      361 gttcatcatt aacagtgaaa tcggtatcca gcaggtaacg atggtgcgga aacagcgacc
      421 agaggatcgg cagaatcgct ttgttgccgg ggatcaccgt ccacagcggc tcaaagacca
      481 gcacttccgg gcgcagcaat acgtcgataa gacgcacttc gttttgcgga tgaccggtac
      541 ggattggcac cgcagcaaac tcacggtcgc taacttcacg aatctgatca aacgcggttt
      601 cccacgccca ggttttccac acgcagttaa ccagtcgccc ttccccatca atcagttgcc
      661 cggcagcatc ccagcccagt tcatccaatc cacgcaagat acgcgtttca aagcccgcct
      721 ggtgcagcgc ctgctccata aactgcgcgt gatagttttc ctcgatatct ttgtcctgca
      781 tgatatggac aaacggacgt gcacgactgt gtttccaggc accagccaat tcgttaatca
      841 gcccttccgc cggattgaag ccgttgcctt tatagccctg ctccgcccaa cgttcgagga
      901 tcaagcccgc ttcggtatga caggaggcgg agtcggcgtt gtactcgtaa accttcaggc
      961 cacgctcatc catgcagaaa tccatacgac cagtgatcat atggtgacgg cgacgctgcc
     1021 aggagagacg caaacgtggc cagaggattt tcgggatgtc gaacagcgcc agcaggttgt
     1081 catctttcag caccttgtcg gttgcgtgaa gatacatcag gtgcagctcg ttggtggctt
     1141 taattagctc ctgctcggca ctctcggtaa tggtgtagta atgataagga tcctgattga
     1201 tcacctgacc gttggcctgc acataggcgt tttgcagcgg atctttttca tccagccatt
     1261 taccgtcaaa ctggcctttg ttttccaggc gcgctccgct gattttcagc agctcgcctg
     1321 caatttccgg ctgcggtaag ctgtattcag tatcttccgt ctggatcatc cagcccagaa
     1381 tggtggtgtc atcaaaagtg tctttcaggg tatagcagcc gttttcgacc accatctcca
     1441 gctcacgcgt ccactgttgc ccttgcggca acggggaatg aatcacgttc tgttccgcaa
     1501 tacggacttt gttgccatgc aattgggtaa tgatggcgac atggccagtg tctttaaatt
     1561 caccgccttt atcccagata agaagcgcac ccgcgaccgg cgcacgcggc gagccgttag
     1621 gaaatgcctg caatggcagg atgttgtcat taaccacttc tcgcaggaag cgcagcgaga
     1681 aaatctccca cgccataccc acgtcagtaa agaccacacc gtaattcaga aagagaaaac
     1741 ggcgagcaaa ttcaacgcat tgccacttgt ggcccatata ttcgtcgtcg atatagctac
     1801 ggaatacggc gtcatcttcg tattcctgcg gatcgagaga actgtaatct gaagagtaga
     1861 ttgctacccc acctggggcg tagcccaata atgtcccgaa cggggcatcc tggctggtcg
     1921 ttcctttgct catcacttta cctttaacaa tacaacctaa gcagttggcg taattgttgt
     1981 gctctgatta cctgctcagc gacattaacc ggacagaggt cagactaatc atacacctca
     2041 tcgcagcggc tggcgcaaag ggttaaaaaa ttcacattct gtacagcaag tgacctgcta
     2101 cactgcttca acactaccac tcagaaggca actcactatg acagacaata cttatcagcc
     2161 cgcgaaagtc tggacgtggg ataaatccgc tggcggcgcg ttcgccaata tcaatcgccc
     2221 ggtttctggt ccgacgcatg aaaaaacgct gcccgttggc aaacacccat tgcaacttta
     2281 ttcgctggga acgccgaacg gtcagaaagt aacgattatg cttgaggagc tgctggcgct
     2341 gggcgttact ggtgcagagt acgacgcctg gctgattcgt attggcgatg gcgatcaatt
     2401 ctccagcggc tttgtcgaag tgaacccaaa ctcgaagatc ccggcgctgc gcgatcatac
     2461 gcataatccg ccgatccgcg tgtttgaatc tggttcgatc ctgctttatc tggcggagaa
     2521 atttggctac ttcctgccgc aggatttggc aaagcgtact gaaacgatga actggctgtt
     2581 ctggttacag ggcgcggcac cgttcctcgg cggtggtttt ggccactttt accattacgc
     2641 accggtaaag attgagtacg ccatcaaccg ctttaccatg gaagccaaac gtctgctcga
     2701 cgtgctggat aagcaactgg cgcagcataa gtttgttgcg ggcgatgagt acaccattgc
     2761 ggatatggcg atttggccgt ggtttggcaa cgtggtgtta ggtggtgtgt atgatgccgc
     2821 tgagtttctt gatgcgggca gttataagca tgtacaacgc tgggcgaaag aagtaggcga
     2881 acgtccggcg gtgaaacgtg ggcgtattgt taaccgcacc aacggaccgc tgaatgagca
     2941 gttgcatgag cgccatgacg ccagtgattt cgagacgaat acggaagata agcgtcaggg
     3001 gtaagggttg gtgttcgtcg cagcaagcca tccaggccgg ataaggcgtt cacgccgcat
     3061 ccggcaatcg tgcacaatgc ctgatgcgac gctgccgcgt cttatcaggc ctacgaaagc
     3121 taatcatcac gcgctggtaa tgtcgtaatc catttggcgc agtgcgtcta atgtggcttt
     3181 ggcttcatct tcgtcgatga tgctcatggc aaatccgacg tgtaccagca cccactggcc
     3241 cagtagatcg gcagggttac cttcacaaat cagggcgata ttcacatcgc gcttgatacc
     3301 acatacttca acctgcgcaa gctggtgaat atcttcaccg acagccagca cctggcctgg
     3361 aacgccaata cacattatta actccggtta ttcaacttca atacttttga cgatcagcga
     3421 atcgccggta tcgacacgca accgctcgcc gtgacagagc ggacactgcg catcgtgctg
     3481 atgaatctcc accacctggc tgcaatccca gcaccaggcc tgggcgggtt tatagacgat
     3541 atgtaaatcg cacccttgcg ccaccgttcc gtggcagaca atttcaaaac taaaacggac
     3601 ggcgctctcc tcaacgcagg agagcgcgcc aatttccagc cacacggcgg tgacgcgctt
     3661 aacatcgtgc tgctccgcct gccgttggat aatttcaacg gcgctctggc aaagagacaa
     3721 ctcatgcatt ttcgccactc ctgcgaccaa acagcagggc gcgacgccct gcgtgtggta
     3781 catccggatt cgtgactggc agcgaaagga tcattcgtgc gcagtcatcc gtcaggcgtt
     3841 ggccctcttc aatcgacatg ctgtgcgaaa gcggcgacat cagcgaacag gagagatatt
     3901 gcgaaacacc gtccagttcg ccaacggtaa aggtcatagt gccatacggc agttgcagac
     3961 caattttttc actcactttg cgcagcggcc agagttgatc cgggccgggg aagataactg
     4021 cactcagcat ccacggggtg atcacacacc ccgtccactg accttcgaac agcgtaaaat
     4081 cagaaacata caccggcatt gaaggatgca gaaaagagag atcgtgcatc gaacgccggg
     4141 caatttcttc aaacgctgcc tgtacttgcg ccttcgggga ggtctggaaa cctgctatct
     4201 cttcagtcat gaatcgcctc ccgtgggatg gcttccacgc cagattcacg cagcgcagcc
     4261 agaacctgct caagcgcagg ttcaatcatt gcttcaaccg tcggcgttaa gccgatgtgt
     4321 ggctccagcg attccgggat cacgccgacc agggtcagct ttttcggaaa ctcgccggtg
     4381 aagcgcaggg ccgacaagac gtcggccagg ccaagctgat gcggagagat tttgttggta
     4441 aacaacgccg gaacttcttc atcccgcagg atcatcatcg ttcccggcgc gttctttttc
     4501 gagacaatgg catccgcaat aatcaaatga tcgcgatttg ccatgtcgcc aagcagctcc
     4561 attcccgccg tgccgccatc gaggatctca acataatccg gcagaatgta tcgttgctct
     4621 aacgcttcga caatccgcac accgatggct tcatcggtca gcaaaatatt gccgacccct
     4681 aagactaaaa tacgcattac agaaccttca ctgaaaccac ttcgttgccg tcagcatcca
     4741 ctatgtgtac cgcacaggcc atgcacgggt caaaggagtg aatggtacgc accacttcca
     4801 gcggtttatt cggatcggca accggtgtac ccaccagcga ctgctcgtaa ggaccgacgt
     4861 catcattgaa gttacgcgga ccagagttcc aggttgatgg aacaaccgcc tggtagttgc
     4921 tgatgatacc gtctttaata accatccagt gagagagcat accgcgcggc gcttcgagga
     4981 agccaacacc tttgaattca cccgttgccg gaatgttcgg tttcacgaag gtggtgtgat
     5041 cgcctttgcc gatattggtg atcagtgcac tgtattggtt ttgcaggata tcctgcaatt
     5101 cgcagcagtg aacggtacga ccaataatac ggcccagcgt agagtgcagt tgcgccactt
     5161 ccagcgtgtt accagtcagt ttctgataaa tcgcaacgat ttcattcagt ttgttctggg
     5221 tagattcgcg acctgccgcc agtttcacca gcatattagc cagcggaccc acttctaccg
     5281 ttttgccgta gaaagtcggt gatttcaccc aggaatattt cccgtcgtca gaccaaccat
     5341 cataagccgg aatggtggta ccttcccacg gtgcctgcgg cgcttcgtct ttataccagg
     5401 agtgcttcgc gctttcctga atgcctttga tcaggtattc atcggaatga gaagtgatcg
     5461 gacgatacga ggacagatcc gcattctcaa tgtagccgcc agggaacagg aagctgccgt
     5521 ttttactgtc ggtcgggaat tccggcacgc tcaggtagtt caccgcacct ttaccacgct
     5581 ccagccattc cgggtagaac gcagcgataa ccgcggtatc gaccttgtaa acctgttcaa
     5641 caaagtcgct cagcttgtcg atgaaagact tgatgtacat caggcgctca aggttcagca
     5701 cgcccagacc gtcgaggttg attgggttag cgaccccgcc taccgccagg ttctgaatgt
     5761 gcggcgtttt accgcccagc agcgccacga cgcggttagc gtcacgctgg cactccagcg
     5821 cctgcaggta gtgcgctacc gcaatcaggt tcacttccgg cggcagtttc attgccgggt
     5881 gaccccagta gccgttggcg aaaatcccca actgaccgct ggcaaccaga tctttgatct
     5941 tgttctgaac tttggtgaac tcttccgggc tgttcaggtg ccaggtcgaa acgcctttca
     6001 gcatttcaga ggctttggtt gggtcagctt gcagtgcaga agtgatgtcc acccagtcca
     6061 gcgccgaaag ctgatagaaa tgaacgatat ggtcatgcgt ggtgtgcgca gccagaatga
     6121 tgttacggat gtattgcgcg ttaaccggaa cgtcgatatt cagcgcactt tctgccgcac
     6181 gaacggaaga cagcgcgtga gtggtagtac atacgccaca gatacgttgc acaatcatcc
     6241 atgcatcgcg cggatcgcgg tttttcacga tctcttccat gccgcgccac atggtaccgg
     6301 aagcccatgc tttcgaaacg acgccatttt cgatttcgca atcgatgcgt aaatgcccct
     6361 caatacgggt taccggatca atagtaattc tctggctcat gctttgctcg cctcatgacg
     6421 attatgatcg ttttgtttta aaggaggaag tatcggcagt agacgaatga gtacgatgta
     6481 agagcaaatc tcaatagcca caaaaccaat agaaatcaac agttcttccc aggtcgggaa
     6541 gtaggcgtaa ccgccgcccg ggttgaatgc caccagcgaa taggtcagac gccaggttgc
     6601 acaacctaac agtgcgctca gtgctgacag gaacagcatg cgggaatcat tacgcagctt
     6661 cgccacacgc agaacgacca gcgggaagag catcagcagg acttcaatcc agaacatcac
     6721 ggagtagaag tcaccggcaa acgctaacga cagcttgtcg cgatagataa gctcgccaaa
     6781 gcgcagcacg atgaaaatcg ccagcaacac actgatggtg ttggtcagct tgacgaacag
     6841 actcttttca tccggaccgt tgccacgcag acccgcctgc accagcgaac cttcaaagat
     6901 gacaatcgag aagcccatga tgaacgccgt cagcagcgag aacagcggca acatttcata
     6961 gctctgccac aacggatgca ccttgtagcc cgccgagatc atcagtgacc ccattgaaga
     7021 ctggtgcatg gtcggcaaca gcgcgccaag tgcaatgatg aagaacatca ccttgttcag
     7081 acgctgtagc gacaccttcc agcccagacg ttcaaacagt gccggagcaa actccagagc
     7141 catcacgccg atgtagatgg tcatacagac cgccgtctcg aacagtaccg agttcacgtt
     7201 gaagtgaccc ggaatgtaga agtacggcag gttccagtag cgacccacgt cgatagtgat
     7261 cgacaaacca cccagtgagt aaccaaacag actcgccaac agcgccggac gcaccagcgg
     7321 atggtattgc ccacggttaa agacgtatac cgcccacgcc agcgcccagc cgccacaggc
     7381 aaagccggtg ccaatcagca ggtcaaacgc gatccacacg ccccacggga agccgccgtt
     7441 caggtcagag acagagccca gaccgaacac cagacgcttc acaatcagga gcatacagat
     7501 gacgattaac ggtccaaaaa tcatgaccgg tttactgatg attttgccgc ccagcggttg
     7561 tggatcatga ctcatgatcg tctcctccgt cgtgatggtc gtttttggtg ttgcgacgaa
     7621 ccagcacggt taagcccgcc agcacagcca gtggtagcat catgccttta tacagggtgt
     7681 gttgaatatt ttcggaacgc gcaccggtag aaagatcgtc cagtttcggc aggtcgagat
     7741 tttcataagg cacacccgtc agtaccagaa cctgagtacc gccgccctct ttctcgccgt
     7801 acagatgcgg ataatattgc ggcaccgtat gcaggtaagt gtcgccagat ttcagcgtct
     7861 gacgcggata gtggtattcg ctgccaggct tcagcgccag acgttttttc gcctccgcca
     7921 tcagctcttc acgcgtaccg aaaatcaccg cgcccgccgg gcacacttct acgcagccag
     7981 gcagaccgcc tttatcgaga cgttccacac ctttctggtt gcacagctcg cacttatgca
     8041 gcgcaccaaa cgggttgttg tagtcgtact tcggcacgtt gtacggacag gcgaccatgc
     8101 agtaacggca accggtgcac acgtctttgt cgtaatggac aatgccggtt ttcggatctt
     8161 ttttcagtgc agagaccggg cacacagaga cacagttcgg atcgacgcag tgcatacact
     8221 gtttcttaat gtacgcgtag ccgttctcct cctggtcttt gttgacccct gtgccgctgg
     8281 tccacacctg aatgatgtta ttggtatacg gcgacagttt gtcgttgttc gaccaggtct
     8341 gttccccttg cgggttacgt tcagggaaat tgatatcctg acacttggtg acgcaagcct
     8401 ggcagcctac gcacaaggtc gagtcgtaca acatccccag cgatcccgga attggcgggc
     8461 ggttttcagc agccgcatga ctgacagacg gcagcgcgcc cgtcagcaat gccccgcagg
     8521 aggctgcttt aataaaatta cgtctgttca cggttattct ccccgtgagt cagcgttatc
     8581 tttcttttgc tgacgaccca gttcacgcac cgccatcacg ctgacaccgg caaccaaccc
     8641 aaccacaccg ccgagcaaac caatagcgcc tgcagagacg ttgccgccct ctttagcgtt
     8701 aacatccggt ttctgtgaac gcggagtttg attttcgacg ttggcaagct gatggatgcc
     8761 tttatggaag ccgatacctt cttcgttaca gccatagcac gggtgaccaa tcgccaccgg
     8821 ccacacaccg ccaacatcgc agaattgcag cgttgagcag ttgccgtaag tttctggccc
     8881 tttacagccg aggtggtaca ggcaccagcc ttcgcggtgg ccttcatcac cgaactcttt
     8941 ggcaaaacga ccagcatcga agtgcgggcg acgttcgcag tgttcgtgaa tcagacggcc
     9001 ataggcgaag gtcggacggt ttttgtcatc cagtttcggc ggtttgccgt aagtgatgat
     9061 gtgcgcaacg gtcgcgagga agttgtgcgg gttcggcggg cagcccggaa tattgataac
     9121 ggttttgcct ggcagaactt cttgcaggct gactgcgcca gttgggttaa ctccagctgc
     9181 ggcaacaccg ccccacgcag agcaggaacc gatagcgata atggctgctg cgccttccgc
     9241 cgctttgcgg atgtgatcca caatcggctc accggcaacc atgcaataaa taccgttatc
     9301 ttttaatggg atggaaccat ccaccactaa cacatactgc cctttgtact tctcgagagc
     9361 gttatgtttg ttctcttcga cctgatgacc gaaggcggcg gaaagcactt cgtgatactc
     9421 cagagagata gtctccagta cgaggttttc taccgttgga tgcgttgcac gaagcagaga
     9481 ttccgtacaa ccggtgcact cctgcgcgcc aatccagata actggcggac gctgcgggtt
     9541 agtaaccgat tcggccatct ctgcagcggc tttgctactt aaccccatgg tggcggctaa
     9601 tgctgcacaa agcttcatga aatcacgacg gttaatgccg tgagaatgga tgagggtgtt
     9661 atctccagtc atttatagtt attccgttgc gaagacctgg catatatttt gcctcaatcg
     9721 caaaatcaat aatgcgatcg atgcgccatt taccacacat ttattatggt tatcgatatc
     9781 atgatactgc ggcggggcgc taaaacgaag ggaagaagca ttagtgtaat taattaaagc
     9841 aagataatac gtatgtttga tcaattttcg aggcgataac cggccataaa aaagcgcaac
     9901 gtagaacagg aattattctc acgaaagaaa tccattacca tctctttatc cagtccatag
     9961 cggcgcgtca gaatacccgc ttcccaggcg ctaagctggc gatctccggt cttttcgtac
    10021 atccggtgtg agtagcctaa tacaaaaccg cgtttataat cggcacagaa ttttgtcaca
    10081 ttaaccgcac tgtcggcatg cgttgctttt aatcccgcca ttaagccttt accaaaatgg
    10141 ttattcatcg ttcacctcat tcgctatata ttgtcatata tagcgatatt ttaggcaaag
    10201 aacgactttt gcgcacgcta ttttttcatg cttgtttaag acgtaatccc gtaagagata
    10261 aacctatcag gagagatatt tatcgaacca tttcagtgtc ctttgccagg caagatcggc
    10321 ggcagatttg tcataacggg gcgtggaatc attatggaat ccgtgattaa cccccggata
    10381 gatatacgcc tcataaacct tattattggc tttcaacgcc gcctcgtaag caggccagcc
    10441 ctcgttgatt cgggtgtcca gttccgcgaa gtggagtagt aaaggcgctt caatcttcgc
    10501 cacatcggca gtgggtgcct gacgaccata aaacggcacc gcgcaggcca gttccggata
    10561 cgcgacagcc gccgcgttcg atacgccacc gccatagcaa aatccggtaa tacccacttt
    10621 gcctgtcgct tgcggatagc gttgcataaa ctcaattgcg gcaaagaaat cattcatcag
    10681 tttggttgga tcaacctgct gttgcagctc acgaccttta tcatcatttc ccggataacc
    10741 tccaacggaa cttaagccgt caggtgccag ggcgatatac cccgccttcg ccactcgccg
    10801 tgccacatct tcgatatacg gattcagtcc acgattctca tgcaccacca ctacggctgg
    10861 cgttttgccg ctcatctttg cgggcttcac cagataaccc cgcacctcgc cgtgaccatt
    10921 tggcgaagga tacgtgatgt actcagcaac aatttccggg tcggtaaact ctacctgagt
    10981 cgccagcgca taatttggct tgagcaaatc aaacaacgct aatgccgtca tcccgcctac
    11041 cgcacttcgc cgcaaggttg aggaactcac gtttcgagat tttcccgtga gcgtaatagt
    11101 catagtaatc cagcaactct tgtgggaaat ctttggcggt taaacgcggc atcgttgcac
    11161 tcctcagttg gtgttttttt aagcaaagca taagcacgtg tttttgccca gtttttcgtc
    11221 actctgtgag ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt
    11281 cgaaattgaa cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg
    11341 gcagatgcaa taccgctatt gcggaaaaag tggtttacgc ctgcccgcgt tatcgctcgg
    11401 tttatggcac aatttcggtc acgttaacgc gctggaatca cagcgtgcga tcctgcgtaa
    11461 agcgtttgat ttgggcatta cgcactttga tttagccaac aattacgggc cgcctccagg
    11521 aagcgcagaa gagaactttg gtcgcctgct gcgggaggat tttgccgctt atcgcgatga
    11581 actgattatc tctaccaagg ctggctacga tatgtggccc ggcccttacg gctctggcgg
    11641 ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta
    11701 tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc
    11761 tgcgctggct catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc
    11821 gccagagcgg acgcaaaaaa tggtcgagtt gctgcgcgag tggaaaattc cgctgttaat
    11881 tcatcaacct tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc tgctggatac
    11941 cctgcaaaat aacggcgtgg gctgtattgc ctttactcct ctggctcagg gattgctgac
    12001 cggaaaatat ctcaacggca ttccgcaaga ttcacggatg catcgtgaag ggaataaagt
    12061 tcgtggtctg acaccgaaaa tgcttaccga agccaacctc aacagcctgc gcttattgaa
    12121 tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa
    12181 agatgatcgc gtgacgtcgg tattgattgg tgccagccgc gcggagcaac tagaggagaa
    12241 cgtgcaggcg ctgaataatc tgacatttag caccaaggag ctggcgcaga ttgatcagca
    12301 tatcgccgat ggcgagctga atctgtggca ggcgtcttcc gataaatgac ctgttaataa
    12361 cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat
    12421 agcccatcac aaatgcagaa agcacaaacg tcagatggat aatgacgtac cacatcagtt
    12481 tgttatccgg gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg
    12541 ccacaatcga cgctgctact ttgtttttca gcgacgttgc gtccattttc cccagccagt
    12601 tcagcttctc tttgttctcg gagatatcca gctgtgagac gaaattctca taaccggaaa
    12661 acatcaccat caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga
    12721 ggatcaaatc tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga
    12781 agaacttcag cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca
    12841 gccagcgaga agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag
    12901 ttaaaacggg caacagtata tcgcaattga gtaaacagca cgcaacagca aatcaacata
    12961 tacgttaaac ctttgtcgca taattctgag gtttagggat cttcctggtt cccttatcaa
    13021 cgcatcgaga acgtctaccc ttaaaggacg ttgaacctga agggagaaaa acgatgtctc
    13081 atttaaaaga cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga
    13141 cgccaggcat ccaggcgaag atgacaccgg taccggattg cggcgagaaa acctatgttg
    13201 gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc
    13261 gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg
    13321 tggaagaaga agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg
    13381 ttctgctgcc aggcgattta agcgatgaga aatttgcccg ttcgctggtt cacgaagcgc
    13441 acaaggcgtt aggcgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc
    13501 cggatattgc agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg
    13561 cgctgttctg gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtatcatca
    13621 ccacttcgtc aatccaggca taccagccaa gtccgcattt actggactat gcggctacga
    13681 aggcggcgat tctgaactac agccgtggct tggcaaaaca ggtcgcggag aaaggtattc
    13741 gggtgaatat tgtcgcgcca ggcccgatct ggacagcact gcaaatttcc ggcggacaaa
    13801 cgcaggataa gatcccgcag tttggtcagc aaacgccgat gaaacgtgcg gggcaaccgg
    13861 cggaactggc ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag
    13921 aagtgcacgg cgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag
    13981 caaccgggca tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct
    14041 acaaacgcat gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc
    14101 atcaggcatt gagcacctaa tgccggatgc gtcgtgaacg ccttatccgg cctacaaacg
    14161 cacgaagatt caacatgttg caatagattt tgtaggcctg atgagcgtag cgcatcaggc
    14221 aattctactt tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc
    14281 cgcctgatgc agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttgtccgc
    14341 tcggaagaag atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat
    14401 tgtttcatcg gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag
    14461 ataaaccggt ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt
    14521 cacatctacc gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac
    14581 gtcgataaac ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt
    14641 aagatgcatt gccatcggat attatcctgc gcgtaatttt tgtgcgacac gaaccggatg
    14701 cgcagcggcg ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc
    14761 aacatcaccc agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac
    14821 gaccgcagga atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc
    14881 cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat
    14941 gatgccccag accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag
    15001 atagccgtta ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa
    15061 gctggtacgt tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt
    15121 ctgcgcttca ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc
    15181 gttggcctgg tttaaggaac gcgcttcagc cagcagttgc tgctcgcgct taaggcgacg
    15241 cttctgattg aagaattcta cgctcttact gaagaagatt gcccaagtga ctacggaggc
    15301 caaaataagc ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca
    15361 gacggaaagg tccgtctgca tcaaattatt acccactgtg tatctccagg acgcaagtca
    15421 caaaatctgc gcataataat atcaaaatga cgtcgaattg atagtcgttc tcattactat
    15481 ttgcatactg ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca
    15541 gcacgccatt tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata
    15601 ttcatgctag tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa
    15661 agcttgatac tcaactggtg aatgcaggac gcagcaaaaa atacactctc ggcgcggtaa
    15721 atagcgtgat tcagcgcgct tcttcgctgg tctttgacag tgtggaagcc aaaaagcacg
    15781 cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt
    15841 tctccttaca acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct
    15901 gcggggcggc agcggttgct aattccattc ttgcttttgt cgaacagggc gatcatgtgt
    15961 tgatgaccaa caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac
    16021 tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc
    16081 agccaaacac taaaatcgtg tttctggaat cgccaggctc catcaccatg gaagtccacg
    16141 acgttccggc gattgttgcc gccgtacgca gtgtggtgcc ggatgccatc attatgatcg
    16201 acaacacctg ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta
    16261 ttcaagccgc caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt
    16321 gcaatgcccg ttgctgggag cagctacggg aaaacgccta tctgatgggc cagatggtcg
    16381 atgccgatac cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc
    16441 aacatcatga aagcagtctg aaagtggctg aatggctggc agaacatccg caagtagcgc
    16501 gagttaacca ccctgctctg cctggcagta aaggacacga attctggaaa cgagacttta
    16561 caggtagcag cgggctattt tcctttgtgc ttaagaaaaa actcagtaat gaagagctgg
    16621 cgaactatct ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat
    16681 cgttgatcct ggcgaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg
    16741 attttagcgg gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg
    16801 ccgatctgga cgccggtttt gcgcgaattg tataacattg ccacttttgg acaattttgc
    16861 agacatttaa ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga
    16921 atcaggagta caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct
    16981 gttattcaag atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct
    17041 catattgtta gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg
    17101 ctgcccgcct catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc
    17161 cagggggtta tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc
    17221 tgctggctaa gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg
    17281 ctggcacagc ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt
    17341 ctgctggcgc tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg
    17401 gcgggaattt ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagtggattg
    17461 ctgtgggtca gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa
    17521 cgccatgaag atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct
    17581 ggcttgttag gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt
    17641 tccccgttcc cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct
    17701 tgccgcatct tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga
    17761 tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat
    17821 catcatccgc gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt
    17881 gacagactta aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg
    17941 ctcgacgctc aggttttcgg tgtatttatt ctcaatccgt ttcagcacgc ggctaatcag
    18001 actgaagtga gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag
    18061 cacgtagtac agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg
    18121 ctccatcacg tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga
    18181 gttaatcccg ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa
    18241 cagttcctgt aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga
    18301 cgtggcatag gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc
    18361 atcataacga aacacgcgtt cattgatata accgatttta tgcccggaaa agagaattat
    18421 gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggt gtctcgccat acaacaaacg
    18481 cacatcgggc aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc
    18541 cgccagcaag cggcaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg
    18601 gcaatttgat tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga
    18661 cattggcaga aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg
    18721 cagatcgttc tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa
    18781 tctgcacacc ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca
    18841 aattcctcac gatgctcgcg tattgattac ctacggcggc ggcagcgtga aaaaaaccgg
    18901 cgttctcgat caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat
    18961 tgagccaaac ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa
    19021 agtgactttc ctgctggcgg ttggcggcgg ttctgtactg gacggcacca aatttatcgc
    19081 cgcagcggct aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa
    19141 agagattaaa agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga
    19201 atccaacgca ggcgcggtga tctcccgtaa aaccacaggc gacaagcagg cgttccattc
    19261 tgcccatgtt cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc
    19321 gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac
    19381 caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctaat
    19441 cgaagatggt ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat
    19501 gtgggcggcg actcaggcgc tgaacggttt gattggcgct ggcgtaccgc aggactgggc
    19561 aacgcatatg ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact
    19621 ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgat accaagcgcg ctaagctgct
    19681 gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc gatgatgagc gtattgacgc
    19741 cgcgattgcc gcaacccgca atttctttga gcaattaggc gtgccgaccc acctctccga
    19801 ctacggtctg gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat
    19861 gacccaactg ggcgaaaatc atgacattac gttggatgtc agccgccgta tatacgaagc
    19921 cgcccgctaa gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag
    19981 ttcacaacac ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct
    20041 aatccaaccg ttattaagct acaggatggc aatgtcatgc cccagctggg actgggcgtc
    20101 tggcaagcaa gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat
    20161 cgctcgattg ataccgccgc ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa
    20221 aatgcctcag tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac
    20281 aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac
    20341 ctctacttaa tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc
    20401 atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc
    20461 catcacctgc aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa
    20521 cttcatccgc tgatgcaaca acgccagcta cacgcctgga acgcgacaca caaaatccag
    20581 accgaatcct ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt
    20641 cgcgatctgg cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg
    20701 gatagcggcc tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt
    20761 gatgtctggg atttccgtct cgacaaagac gaactcggcg aaattgcaaa actcgatcag
    20821 ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct aacatgcaaa ttctcccggt
    20881 ggcggtaatg ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc
    20941 gttgttactc ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga
    21001 agctcttaaa agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca
    21061 gatgaaaata atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcacagcc
    21121 cccctcacag accgtagaac aaacagtccg gcatatttat cagaactata aatcagatgc
    21181 cactgcccct tattttggtg aaaccggaga gcgggcgata acttctgcgc gtattcaaca
    21241 ggcgcttacc ctgaacgaca atcttacgct gccgggcaat attggctggc tggattatga
    21301 tccggtttgt gattgtcagg attttggcga tctggtgcta gaaagcgttg cgataaccca
    21361 aactgacgcc gatcatgccg atgccgttgt gcgctttcgt atctttaaag atgataaaga
    21421 aaagaccacg cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgacgatat
    21481 tgtcagcaat catggcagcg tcttacaagc agttaatagc gagaatgaaa aaacgctggc
    21541 cgctttagct tcgttgcaaa aagaacagcc ggaagccttt gttgccgaac tctttgaaca
    21601 tattgctgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt
    21661 taatgccttc tataaaacca ccttcaagac ggccaataat cccgatgaag atatgcaaat
    21721 agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc tattttcacg
    21781 cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc atgttcgttt
    21841 tacgctgacc aatggcaaca acgaagagca agaactggtt ttacagcggc gcgaaggcaa
    21901 gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc
    21961 aaaaactgcc gccagattaa agcaatgagc tgaattaaat aacaattagc cggaacaata
    22021 aataaaaggg aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc
    22081 tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc agctggcgag caatgtgcaa
    22141 acattaaatg ccaaaatcgc ccggcttgag caggatatga aagcactacg cccacaaatc
    22201 tatgctgcca aatccgaagc taacagagcc aatacgcgtc ttgatgctca ggactatttt
    22261 gattgcctgc gctgcttgcg tatgtacgca gaatgataaa aaaatccccg gtagcgtgtc
    22321 agttgccggg gatatttttt aacgtccaac cgccgcttta ggccgtttct tcgcaccagc
    22381 attcaccgga cgagattgcg tagacgacgc tttttttgcc gtagcaggcg tctgacgctg
    22441 ggtcgccatc ggcgtatgtt tcgtcaacgc cggacgggta ttgcggttct ggcgacgagc
    22501 ttcacgcatc tcttcaatgg ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag
    22561 atgctttttg cccatcgctt ccagcgcctg gcggattaac ggccagtttg ccggatcgtg
    22621 gtaacgcaac aacgctttat gcaaacgacg ctgtttgtcg cccttcggta cgaagacgtc
    22681 ttcgctctta taaccaatct tcgccagcgg gtttttcccg gtgtaataca tggtggttga
    22741 gttcgccagc ggcgacggat agaagttctg cacctggtcg aggcggaagc ggtgcttttt
    22801 cagccacagc gccagattca ccatatcttc atcacgcgta ccggggtgcg cggagatgaa
    22861 ataggggatc agatactgct ctttacctgc ctgttttgag taagtatcga acagctcttt
    22921 aaagcggtca tagctgccca tgcccggctt catcatcttc gataacggcc cttcttcggt
    22981 atgttccggg gcaatcttca gataaccgcc gacgtgatgg gtcgccagtt ctttgatata
    23041 gcgcggatct tctacggcta tgtcataacg cacaccagag gcaatcagga tctttttaat
    23101 gcctttcaga tcacgcgcac ggcgatagag gttgatcgtt ggttcgtggt tcgtgtccat
    23161 gtgcggacaa atatccggat aaacgcacga caaacggcga caagtttgtt cagcgcgtgg
    23221 cgatttgcag cgcaacatat acatgttggc agttggccca ccaagatcgg aaatcacgcc
    23281 cgtaaaacct ggaacggtgt cgcggatcgc ttcgatctca ttaatgatcg aatcttcgga
    23341 acggctctga ataatgcgcc cttcgtgctc ggtgatagaa cagaaagagc agccgccaaa
    23401 gcagccacgc ataatgttga ccgaaaaacg gatcatttcg taagccggaa tacgggcatt
    23461 gccataggcc ggatgtggca cgcgcttgta tggcagcgca aaaacgctgt ccatctcttc
    23521 ggtagaaagc ggaatagcag gcgggttgat ccacacatag cggtcgccgt gtttttgcat
    23581 caatgcgcgg gcacagcctg ggttggtttc gtggtgcaga atacgcgaag catgggcgta
    23641 cagcacttta tcgcccttca ctttctcgaa agaaggcagc aacacgtagg ttttttccca
    23701 cggtttcggg cgcggtggct gcacggttac ggctttggct tcctgctttt tcggtgccac
    23761 cggtttgtta tccgcgcacg gcaaatcttc accatacgga tgcgggattg ggtcgatttt
    23821 tccaggggta tcaagacggg tggaatccac gccgctccag ccaggcagcg cctctttcac
    23881 gataatcgcg gtattacgca catcgcggat ttcactaatc ggctcgccca tcgccagacg
    23941 gtgcgccacc tccaccagcg gacgctcacc gttaccaaac atcagcatgt cggctttcga
    24001 atccaccagc acggaacggc gcacggtatc ggaccagtaa tcataatgtg cggtacggcg
    24061 cagactagcc tcaataccgc cgaggatcac cggtacatct ttccacgcct ctttacaacg
    24121 ctgggtataa accagtgtgg cgcgatccgg gcgcttaccc gcgacgttat ccggcgtgta
    24181 ggcatcgtca tgacgtaaac ggcgatcggc ggtataacgg ttgatcatcg aatccatgtt
    24241 gccagcagta acaccgaaaa acagattcgg tttacccaga cgcatgaaat cgtctttgct
    24301 gctccagtct ggctgggcga tgatcccgac gcgaaagccc tgtgcttcca gcatacgacc
    24361 gcaaatcgcc atcccgaagc ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat
    24421 gtcgcagcta tcccagccaa gttgatccat ctcttcacga gacatcggca aaaacggtgc
    24481 cggtccaaaa caggcggccc agtactgcgg ccaggagaac aggtcgcgat ccggttggat
    24541 cagggagata gagctcattt tgcttccaga aatgataaaa aaataatcaa aggccgggga
    24601 ttataagccg gaacgaaaga gaaatcgaaa agtattccat actcgccctc ctcgggcgag
    24661 tatgaagatt acggtaccgg attgactaac agttgcccaa tcgagccacg gtccgccatt
    24721 tccagcgtct gactgttgaa gtagaacggg aagtgcgccc aggaaggctg accgaaataa
    24781 acaagcagct ccacttgtcc gtcaacccaa acggtatctt tccagcctct gtcttccggg
    24841 aacggcatcg cgccattcac gttacggatc tggaacatta cgccttcaat atggaacgct
    24901 tgcggctcgt ccgcgcgtac cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca
    24961 atacggttga cgtcccacag ctgtccatta atacccgggt catcacccag actgatatcg
    25021 cgactgcgaa ttggcgaacc agccatgatt tcagttggca gcaagcgcat cggaagactg
    25081 tctgtgacca gcggcagaag gccggttggg cgcagcgtta gcaccagggt agaaaccaga
    25141 atactggatg gctcaaagaa gccacgaata cgatcaacaa tgctcgccgc ttcgccacag
    25201 gtgatcgaca cttcatcgcc gttgctcata tccaccagaa tctcgcggcg ctcgcccggt
    25261 gccagcgaaa gttgcttcac cgatacagga gcaggcagga atccctgatc gccagaaatc
    25321 acatgtaacg ggcgaccatc gttcatctgt agttgatagc gacgagagtt cgacgcgttc
    25381 agcagtcgca agcgcaccca gccacgcgag acttcaacgt acgggctttg tacaccgtta
    25441 accagcagcg tatcaccaac aaagccgccg cttcccggtt cgttgtattc tggcgtacca
    25501 aagttatcca gccgtttatc ctggataatg accggaaaat catccacacc ataatggttg
    25561 gggataggca gcgacttgct gacttcatct tccaccagcc acattccggc aaggccgtta
    25621 tagacctgct gagccgtgcg gttgggagta ttggcgtgat accacagagt agctgcgttc
    25681 tggcgaatgg gcagtacggg tgcccagtca gcgtttggcg acatcatccg tgccggaccg
    25741 cccatcagcg ggcctggtac ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg
    25801 cggttgctgt aaataagctt aacatcgtcg cccttccaga cgcggatagt cggccccagg
    25861 taacgaccat tgattcccca gaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca
    25921 cgttgtacag tcataaacag cggttgccca cggcgagatt caagtagcgg cggaacgggt
    25981 agcggttgct gttgcccggc tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca
    26041 atccccgatg cctgaatgaa ctgacgccga ctgagtgaca tatttgctcc atgtaaaact
    26101 ggctaaagta ataccgtgca ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa
    26161 cttttccggc ggcttcgcgt tctgcgactt ctttatcgag ctcggcgatt ttttgttcca
    26221 ttatcgaacg acaatgggca gccagctcac gaacctgatc tttgccatac tgactgacgt
    26281 caattggcgg cagcatttcg acaatcacca gaccgttgtg cagtcgatta agattaatct
    26341 tattcgaagt tgtagagacg cacacgggaa taatcgggac gcccgccgca attgccgcgt
    26401 gaaatgctcc agtcttgaac ggtagcaggc cgcgaccacg gctgcgggtt ccttccggga
    26461 acatccagat ggaaatacgg cgttttttga agtgattcac tacttccgca atggtgccgt
    26521 gagctttagt gcgattgttt ctgtcgatca ataagttgcc ggttaaccag tacaactgcc
    26581 cgaagaaggg gatccacagc aagctctttt tacctaccgt caccgtcggc ggttgcacga
    26641 tgttcgatgc tgtcaccatg tcatagttgt tctggtggtt agcgatatag atagcattgc
    26701 cgtagctttc agcgtctgta ggtttacggc actcaacttt caggccaaac agcggcgcaa
    26761 gacgaccaaa catatgccca aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc
    26821 agtaaatgga gccgaataca cagactaaga tgctgtaaat cacggtaata ataagacgaa
    26881 agatatatag cataacaccc tctgaaggtc tgagagactg gcattgtacg tcacctgaaa
    26941 tcaggttagg gcattgttaa aagccgctct ctggaaaagc gtattgttaa tcgcaacgca
    27001 tgaataaaca acggtttcaa gggaattttt atcgtttccg ggggttgaat acgctgccgg
    27061 atgacgactt aacgtttcat ccggcgttcc ttgcaagcgg gaggaaacag cgccctcccc
    27121 ggcatattac tcttcgctat caccgctgct ggcacggcga ggagagtcga tctcaacacg
    27181 atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcgc cagtgacttt
    27241 ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct
    27301 ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg cttctgccga
    27361 tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cacttaccgg
    27421 gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat
    27481 caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct tacctgcacg
    27541 gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat
    27601 cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc caggcggcaa
    27661 cgttaatttg ccggtgagcg gctcgccctg accacgcgcc gacggcagcg taatcgggtc
    27721 aatggcatag ctacgaccgg tggaatcaac aaacactacc ggttggttgc tcttaccttt
    27781 caccgccgct ttgaagctat cacccgcttt ataattcagg cccggcgcgt cgatatcatg
    27841 gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag gttcagacgg
    27901 cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg
    27961 atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt
    28021 acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagtt cactctgctc
    28081 accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc
    28141 ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc
    28201 attacgaatg atctcaatca cttcgtcgat attgagaaac gccaccagca aaccttcgag
    28261 gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc
    28321 gcggcggaac accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc
    28381 atccagaccg atcatattaa ggttaatacg atagctcttt tccagatcgg tggtagcgaa
    28441 gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccaggcg
    28501 ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt
    28561 gcgcatttgc gcagcaattt gctccagtac gcgcgcacct gaaacctgat gcggcaatgc
    28621 gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgca ctgaaccacg
    28681 tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg cttcagtcgg
    28741 ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg gctggtcgat
    28801 taatgcgatt gccgcctgag ccacttcacg caggttatgc ggtggaatat cggtcgccat
    28861 gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacggg caggtagcat
    28921 tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag
    28981 ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc
    29041 gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg
    29101 gtaagagaac ggttgcgcca tcaggaccat cgcttcataa caggcgctat cgccgtgcgg
    29161 atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc
    29221 attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc
    29281 accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt
    29341 ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc
    29401 aacttattcg ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg
    29461 tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg gcgataaagg
    29521 catcaatgtg ccttgatata caactctttg ctgtagacat aatcctgggg attatgcagc
    29581 ggaaaaccgc caacgtaggg tttaagcagt ttgattaacg gctgatagta gataggaatc
    29641 agcggtgcct gttggttgat gatcacttct gcctgctgat ataacgcatt acgctttgtc
    29701 gcatcagtga tctgcgtggc ctggtttagt aaggcgtcat actgcgcatt tttccagtga
    29761 ccgacgtttt cttcactatc gcttttgagc gtgttcagga agctggaagc atcattgtac
    29821 gtcgcatccc acgactgccg agacagcatg aaatcaccgg ctcgtcgggc atcaagatag
    29881 gttttccact ccattgtgcg cagcgtcacc tgtgcaccca gccatttttt ccattcggaa
    29941 gacaacgcta tcgcggtctt ttcatgcaga tcgtacttgt tgtagaacag ctcaaagcgt
    30001 agcggatgag aggcgtcgta tcccgcctgt ttcagcaagg cttttgccat cgcgacgcgc
    30061 tcactcattg gcttttgcag ttcatcgaac gtcgtcgcgc taaagccttt tacctctggc
    30121 ggcgtcagcg tggttgcggg cgttctcaac cccagtacct tttgcgcaat aagctgtcga
    30181 tcaaccgtaa gatatagcgc ccgacgcact cgcacatcgt taaatggcgg tttctcaagg
    30241 ttgaagttgt aatattcgct gttcagacgc ggaataattc gtagctcgcc aggcagtgat
    30301 ttttcaatgg cgggaatttg ctgcgccgga acccaggtga gatcgacctt tcccgcgcga
    30361 tagcggttat agccggtgac cgaattatct agcgccagat actcaacctg ttgcaatact
    30421 gtatgttgcg catcgcggta ctttggattt ttgcgtgcag taatcttttc gttaactacc
    30481 cactgatcaa gcacaaaggc accgttgtaa accatgttct ctggcttact ccagctatcg
    30541 ccatgtttag cgatgacatg atgaggaacc gggaacagcg tcggccaggc gagcatcgtc
    30601 gtgaaccaag gaaccggctg ctcaagcgta acttcaagag tacgatcatc cgtcgctttg
    30661 acacccagcg atgtaacatc tgctttaccc gcaacaatag ctgcggcatt gttaatgtgc
    30721 gcctgtgcca gatagccagc aaaagggctt gccgttttcg ggtcaaccgc gcgctgccag
    30781 ccgaggacaa aatcctctgc cgtcagaggc tgaccgtcgg accactgcaa accgctacgc
    30841 agatggaaaa tatagcgctt gccgccgtcc agtatctccc agcgttcagc ctgagcgggc
    30901 tgcacctggc cttcaccgtc catccatacc agaccttcaa acagatccag cacaatctgc
    30961 gcggcagtat tctcctccac cttttgcggg tcgagcgtac ctgggtcgct atgattgttg
    31021 taacgaaaga cttgttgcgg ggcgagcggt gtgttggcgg gaacgtcagc agcgtagaga
    31081 ggagccgcac ttaccaggct gaccagccat aataaatttc gcgtatacat ggtattcctt
    31141 accggagaaa ttataagccc aacaacaata cgcctgtggc attgttcgct caaacttatc
    31201 gcgagtgatt tggctcacac tccggtaaag aaaaaagcga gttgcccccg cttttccatt
    31261 aattaacgga tttcattcaa tagttctgga tgcttatcca gaacacgtaa aagtttgatt
    31321 gtggaaggat gaggttgggc attgcctttt tcgtaacgcg aaaacgcatt tacacctccc
    31381 ccaaaaattt cgcttgcctc tttttgggta agagagagct tttttcgaac cttcactata
    31441 aattcaggtg ccactgtttc ggcattcacc gaagcccgaa atgcctttac ttgcgccatg
    31501 aaagcatctg actcttcttt attcatgatg ctctcttcgc aatggacaca atataaaccg
    31561 tggatacctt tcaatactgt ttttcgtcca cggaaggtgt atggaatatc tttaatgcca
    31621 gaaaccattt ctccctggtg gcaaaccgga catttcatat tacttctcct taaacgagac
    31681 gatcagtacg tcatgaatta ccgtaatttt aagataaacc tggcctgtaa caagcctggg
    31741 tctgtaaaca tcctgccaga tagtatgatc agagtaggtg gtcatgcttt tataaaagtc
    31801 gctctctgat aatccaatga taacattaca cataccatca aaatccaaac ctaactcatc
    31861 tgcatttaac agtgcactac gtgttgtacg aacttgcccg gcattgacaa gttttttaac
    31921 ctgactcaaa cgtgtatgtg gtgtgcgttt ttccataacc cccgcctccc tgttacttta
    31981 gttataacct aaaaggttaa ttacagcaat gaaaaagcac ctaaaaggtt agttagatgt
    32041 acggagatag tgaccacaca aaacgtattc tttaaggaaa gtgattgacc atataagaaa
    32101 gtggcgcatt agtagcgcca gtttgaagca ggaatttata agggaagctg gagtcaggca
    32161 tcacgccaac ggcacataaa catccgtttc cagtcgctgc tctgtcaccc cttcggcaag
    32221 attggtgtag tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca
    32281 gtggcgaatg atgccccata ccgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc
    32341 cacggcataa cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat
    32401 cggttcgcta acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg
    32461 gtcgtcccag gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt
    32521 acgccacatg ataaatttcg ccacgctgta gttgagcaat tcagggctac agcggtgcgg
    32581 caacatcgcc acaggtattg atgggaaatc gataatgttt acatccagtg tcaggtttgt
    32641 cattttgcct ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg
    32701 gatttacttt gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt
    32761 caccctgaat ctcaaccgta tctttcggcg tcaccgtcac gccgttccag cgtttgtggt
    32821 cgatatcaac attgatagta ccgctggcat ctttgaacac gtagagatcg tcagagatgc
    32881 gttcaacgat attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt
    32941 ctacagtcgt tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg
    33001 atgggccaga aaaaccgccc tgctctgctg ccatcaccgg tgcgctgcac agggccatta
    33061 ctgcgattac tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag
    33121 catttaacaa gatagtcctt aacaacttct taagggaaaa aaataaaatt tagtgctgta
    33181 cagagcgcgt tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa
    33241 tgcgaatttt actgatagaa gatgacatgc tgattggcga cggcatcaaa acgggcctta
    33301 gtaaaatggg ttttagcgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt
    33361 atagcgcacc ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg
    33421 atattttgcg cgaatggcga gaaaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc
    33481 gcgatgcgct ggcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta
    33541 aaccttttgc gttgatagaa gtcgccgcca ggctggaagc tctgatgcgc cgaaccaacg
    33601 gccaggccag caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg
    33661 ccacgctggc tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac
    33721 tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct
    33781 gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac
    33841 tcggtagtga ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa
    33901 atttacccaa cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc
    33961 ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga
    34021 attgttcgac acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga
    34081 aatcaacgcg gcggatcgca tggcacagac gccaaataga ttaaaacacg gtcatgttga
    34141 tgacgatgcg ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg
    34201 cgataacgga gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact
    34261 ggtcggtgaa gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg
    34321 catcgttgtt ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg
    34381 gcaattgatc ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact
    34441 gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga
    34501 ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa gtgcggccac tggttgagtc
    34561 gctaaatcaa ctgttcgccc gcacacatgc gatgatggtt cgtgaacgac gctttacctc
    34621 cgacgcagct cacgaacttc gtagcccgtt aacggcgctg aaagtgcaaa ccgaagttgc
    34681 gcagctctct gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg
    34741 gatcgatcgc gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact
    34801 ggataacctt caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat
    34861 ggatatttac cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca
    34921 cagcatcaaa cgcaccgggc aaccgctatt gctaagtttg ttggtgcgaa atttgctgga
    34981 taacgccgtg cgctacagtc cacagggcag cgtggtagac gtcacgctga atgctgataa
    35041 tttcatcgtg agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga
    35101 acgcttctat cgcccacccg gacaaaccgc taccggcagc gggcttgggc tatcgattgt
    35161 ccagcgaatc gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg
    35221 atttgaggcg aaggtaagct ggtaacttct ctgtctatga gagccgttaa aacgactctc
    35281 atagatttta ctaatagcaa aatataaacc gtccccaaaa aagccaccaa ccacaaacca
    35341 gacaaatacc gagcgagaat acatataccc aaaactcgaa catttcccgc ataaagagtt
    35401 ttcttaagat aagaataata agtggcgtaa gaagaaaaaa tgctgcaatc attgcttgca
    35461 atatacgatc ttcgcctgaa acggaaggta tactgcaaat atttcctccc gactcaagca
    35521 tccagtcata cttattgaaa gcaaaaaaag aatctattaa ccaataagta agcattatat
    35581 atagcaatgc ttcaaatatt gttttaattt tctgctttaa catatatcag tacgctcata
    35641 aaaatccatt tataattatc aacatgacac gttgatactt tcatctttta cttattcaaa
    35701 gccatatttt caatccccaa aaaaaccgaa ccaaacgtgt aaattattcc ggtgcgagta
    35761 agaattatga ggaatggcta tcagtattgt cattttcaga aaatatttat cctgcatcgg
    35821 tgagtcagag ctagatcaga cttttgctaa attcgcaaaa gactttgcac attttgctaa
    35881 tttcaccgta ccgctctgtg acgtactata gtcggcaaac gtctcacttt gaggttaaaa
    35941 aatgagcaac atcctgatta tcaacggcgc gaaaaaattc gcccactcca atggtcaact
    36001 gaacgacacc ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc atgatgttcg
    36061 catcgttcgc gccgacagcg actacgatgt caaagcggaa gtacaaaact ttctctgggc
    36121 tgatgtggtg atctggcaga tgccaggctg gtggatgggc gcgccgtgga cagtgaaaaa
    36181 atacattgat gatgtattca ccgaaggtca cgggacgctg tatgccagcg atggtcgtac
    36241 ccgcaaagat ccgtcgaaaa aatacggttc cggcggcctg gtacagggca aaaaatatat
    36301 gctttctctg acctggaacg caccaatgga agccttcacc gaaaaagatc agttcttcca
    36361 cggcgttggc gttgacggtg tgtatctgcc gttccataaa gcaaaccaat tcctcggtat
    36421 ggaaccgctg ccgacattta tcgctaatga cgtgataaaa atgcctgatg ttccccgcta
    36481 tactgaagaa tatcgcaagc atcttgtgga aatttttggt taactagagc tcaggcttta
    36541 gaaggagtta accatgctta ccgtaatcgc agaaatccgt actcgtcctg gtcaacatca
    36601 ccgtcaggcg gtattggatc agtttgctaa aatcgttcca accgtactga aagaagaagg
    36661 ttgccacggc tatgcgccaa tggtggattg cgcagctggc gtgagtttcc agtctatggc
    36721 accggattct atcgtgatga ttgagcagtg ggaaagcatc gcgcatcttg aagcgcatct
    36781 gcaaaccccg cacatgaagg cgtatagcga agccgtaaaa ggtgacgtgc tggagatgaa
    36841 tatccgtatt ctgcagccag ggatttaatc ctgccttgtt tgcccggcca tcctgaccgg
    36901 gcaatgttct ttcctttaaa cctcaatctc cgccatgtcg cctttctctt gcaaccagtt
    36961 gcggcgatct tccgagcgtt tcttcgccag cagcatatcc atcatcgcgt cagtacgctg
    37021 atcgtcttca tcatcgatag tcaactgcac cagacggcga gtattcggat caagcgtggt
    37081 ttcgcgcaat tgcatcgggt tcatttcccc cagaccttta aaacgctgga cgttcggctt
    37141 gcctttcttg cgttttaatt gctcaagtac gccctctttc tcttcttccg tcagcgcgta
    37201 ataaacctct ttcccgagat caatacggta gagcggtggc agtgcgacgt aaacgtgacc
    37261 gtgtttcacc aacgcgcgga aatgttttac gaacaaagcg cagagcagcg tggcaatgtg
    37321 cagaccatca gagtccgcat ccgcgaggat acagattttg ccataacgaa gctggctcag
    37381 atcgtcgctg tcaggatcga taccgatcgc taccgaaata tcgtgcactt cctgcgaagc
    37441 cagcacttcg tcggaagaga cttcccaggt gttaaggatc ttacctttca gtggcatgat
    37501 cgcctgatat tcgcgatcgc gcgcctgctt ggcagatccg cctgcagagt caccttccac
    37561 aaggaacagt tcggtacggt taagatcctg cgcggtacag tcagccagtt tgccaggcag
    37621 cgccgggccg ctggtcagct ttttacgcac cactttttta gccgcacgca tacgacgctg
    37681 ggcgctggaa atcgccatct ccgccagcag ctccgccgcc tgaacgttct ggttcagcca
    37741 caggatgaag gcatctttca ccacgcccga aacgaatgcc gcacactgac gcgaagagag
    37801 acgctctttg gtctgcccgg caaactgcgg atcctgcatt tttactgaca gcacataggc
    37861 gcagcgatcc cagatatctt ccgccgacag cttcacgccg cgcggcagaa tgttgcgata
    37921 ttcacagaac tcacgcatcg cgtccaacag gccctgacgc aggccattaa catgggtacc
    37981 gccctgcatc gttgggatca ggttgacgta gctttcggtc agcagttcac cgccttccgg
    38041 cagccacagt agcgcccagt ccacagcttc agtatcgcca gcgaaattac cgataaacgg
    38101 tttttctggc agcgtcggta aaccgtttac cgcttccgcc aggtaatcat tcagaccgtc
    38161 ctgatagcac cagcgctgtt cggtgttgtt gatctcatct ttaaaggtaa tctcaacgcc
    38221 ggggcacagt accgctttgg ctttcagcac atgcgtcagg cgtgaaacag aaaatcgcgg
    38281 gctgtcaaag aaggtttcat ccggccagaa gtgcacgctg gtaccagtat tgcgtttacc
    38341 gcaagtgccg acaacctgta aatcctgcac cttttcgcca ttttcaaagg cgatgttata
    38401 aacctgacca tcgcggcgca cgttaacttc tacgcgcttc gacagggcgt taaccaccga
    38461 aatccccacg ccatgcaggc cgccagagaa ctggtaattt ttgttagaga atttaccgcc
    38521 tgcatgcaga cggcaaagaa tcagttcaac cgccggtaca ccctcttccg ggtgaatatc
    38581 caccggcatc ccgcgcccat cgtcaatcac ttctaacgac tggtcagcat gtaagattac
    38641 atccacgcgt tttgcgtgac ccgccagtgc ttcatcgaca ctgttatcaa taacttcttg
    38701 ccccaaatgg ttagggcgag tggtatcggt atacatcccc ggacggcggc gaaccggctc
    38761 aagcccggtg agtacctcaa tggcatcagc gttataagtt tgcgtcatgg tttaagttag
    38821 caattcgagt tgatcgtcag agatggtgca gaccaagaaa atcgacgatc gggttgaaat
    38881 aatcttcgaa gcccgtgaat gcgtggttgc cgccttctat gacagtctgg cggcaggaag
    38941 cgtagtacgc caccgcctgg cggtaatcca gcacttcatc tcccgtctgt tgcagcagcc
    39001 agatcaaatc cggcgcttcc agcgggtcaa tctgcatgac tttgagatcg taaatatggc
    39061 gtgactctag cacatattgc tgcccggtgt aggggttctc gttctgaccg agatagtccg
    39121 tcagcagttc aaacgggcgt accgccgggt ttaccaccac tgcgggcagc ataaaacatt
    39181 gcgacaacca ggtggcgtaa tatcccccca gtgacgaacc gacaataccc agcgaatcgc
    39241 cgccatgttc caggacaatg gattccagca gttctgccgc gtcggaagga tacggcggca
    39301 actgcggaat gatcatctca acgtcagggt gatgttccgc cagccagttt tttaacaagc
    39361 tcgcttttgc agagcgcggc gagctgttga aaccgtgtaa ataaagaagc gtagacatca
    39421 gtagccttct gaagcggtat caggttggaa acgtgtgtcc gccaggcgat gcacctcggt
    39481 ggtcagcgtg ccatcagcat gtaactcgag agtacgccag ccgggcgcga tggtgtccag
    39541 cgtaaagttg gaacagtgcg gcttaaactg cacacaggtc gacggcgttg ccagcaggcg
    39601 gcgaccgttc cagtcgagat ccagctcctg atgaatatga ccgcacagca agtatttgac
    39661 gtgcggaaac ttcgccagca cggtatccag ttcgcccgcg ttacgcagac tgtgttgatc
    39721 gagccaacta caacccgcag gtagcggatg atgatgcagc agcagcaacg tatggcgttc
    39781 tggcgcatcg gccagtttac gttccagcca ctcaagctga aactcgctca gctcaccgtg
    39841 cggcacgcca aacacctggc tatccagcaa caggatttgc cattgctcac caataaacac
    39901 gcgcttcgcc ggggagatac ccgcatcctg taacgcgctg tacatcgcgg gctggaaatc
    39961 gtggttgccc ggcagccaga cgcagggcgc acgaaaactt gcgatgcctt cagcgaaatg
    40021 ctgataggcc gcagaggatt gatcctgcgc taaatcacct gtcgcgacaa tcaggtcgaa
    40081 ttcgtgctga tgtggacgaa tcgcctccag caccgcctgg tagctttccc aggtgtttac
    40141 ccctaacagg gcttcgtgct tttgtgcaaa caggtgagta tcggtaattt gtaaaatcct
    40201 gactctggcc tcaccagcca gaggaagggt taacaggctt tccaaatggt gtccttaggt
    40261 ttcacgacgc taataaaccg gaatcgccat cgctccatgt gctaaacagt atcgcaacca
    40321 gtccgctaaa aactgattaa tttgatgctt ttcgtcgcgt tgatgcaact ttttattagg
    40381 ataatcatac cgcgctttga agcgaaaaat ctgctggctt gaacacactt cagccaccat
    40441 cgcgtcatga tacagacgca ccgtcattga cggaaggctc cagtaactga tcgcgggcgc
    40501 agtctgttct attgtcacta gggtagtgta tcgggtcgat tccacaatcg tcagccgata
    40561 ttgtgcgttt gccacctgat agcttacagt ttcgccgggt gcgtcattgc gcggtaacaa
    40621 acggcgcaat tgtgaaaagt tcatctcgca caggcgcatc atttcaggaa agtcaggtgt
    40681 gtaacgcttc atttatgccc actcattttt taacgcttga tgatgcagct gcagccattg
    40741 caaagcgatg accgacgctg cgttgtcgat tttcccctct tctacccact ggtatgcctg
    40801 ttcccggctt accacatgaa cgcgaatatc ttcgttttca tcagccagac catgaatacc
    40861 gcttgcggtc gtggcgtcca cttcgcccac cataattgac gaacgctcac tggtgcctcc
    40921 cgggcttgcc aggaaactta acaccggttt ggtccgtttg actatcagtc ccgcctcttc
    40981 aatcgcttcg cgacgggcaa catcttccac actttcaccc tcttcaatca tcccggcaac
    41041 catctccagt agccaggggg tttcactggt gtcgtatgcg gcaatccgaa tctgctcaat
    41101 cagcacaact tcatcacgca ctgggtcaaa gggtagcaag actgcggcgt gaccgcgctc
    41161 aaaaatttcc cgccgtacct catgactcat ttgcccgttg aataaacgat gacgaaatct
    41221 ataaagatct aatgaaaaaa agccgcgata aagtgtttct cgtgcaataa tttctacatc
    41281 gtttttgcca aatgtaacgg gcaggttgtc tggcttaagc attgttaatg tcctggcact
    41341 aatagtgaat taaatgtgaa tttcagcgac gtttgactgc cgtttgagca gtcatgtgtt
    41401 aaattgaggc acattaacgc cctatggcac gtaacgccaa ccttttgcgg tagcggcttc
    41461 tgctagaatc cgcaataatt ttacagtttg atcgcgctaa atactgcttc accacaagga
    41521 atgcaaatga agaaattgct ccccattctt atcggcctga gcctttctgg gttcagttcg
    41581 ttgagccagg ccgagaacct gatgcaagtt tatcagcaag cacgccttag taacccggaa
    41641 ttgcgtaagt ctgccgccga tcgtgatgct gcctttgaaa aaattaatga agcgcgcagt
    41701 ccattactgc cacagctagg tttaggtgca gattacacct atagcaacgg ctaccgcgac
    41761 gcgaacggca tcaactctaa cgcgaccagt gcgtccttgc agttaactca atccattttt
    41821 gatatgtcga aatggcgtgc gttaacgctg caggaaaaag cagcagggat tcaggacgtc
    41881 acgtatcaga ccgatcagca aaccttgatc ctcaacaccg cgaccgctta tttcaacgtg
    41941 ttgaatgcta ttgacgttct ttcctataca caggcacaaa aagaagcgat ctaccgtcaa
    42001 ttagatcaaa ccacccaacg ttttaacgtg ggcctggtag cgatcaccga cgtgcagaac
    42061 gcccgcgcac agtacgatac cgtgctggcg aacgaagtga ccgcacgtaa taaccttgat
    42121 aacgcggtag agcagctgcg ccagatcacc ggtaactact atccggaact ggctgcgctg
    42181 aatgtcgaaa actttaaaac cgacaaacca cagccggtta acgcgctgct gaaagaagcc
    42241 gaaaaacgca acctgtcgct gttacaggca cgcttgagcc aggacctggc gcgcgagcaa
    42301 attcgccagg cgcaggatgg tcacttaccg actctggatt taacggcttc taccgggatt
    42361 tctgacacct cttatagcgg ttcgaaaacc cgtggtgccg ctggtaccca gtatgacgat
    42421 agcaatatgg gccagaacaa agttggcctg agcttctcgc tgccgattta tcagggcgga
    42481 atggttaact cgcaggtgaa acaggcacag tacaactttg tcggtgccag cgagcaactg
    42541 gaaagtgccc atcgtagcgt cgtgcagacc gtgcgttcct ccttcaacaa cattaatgca
    42601 tctatcagta gcattaacgc ctacaaacaa gccgtagttt ccgctcaaag ctcattagac
    42661 gcgatggaag cgggctactc ggtcggtacg cgtaccattg ttgatgtgtt ggatgcgacc
    42721 accacgttgt acaacgccaa gcaagagctg gcgaatgcgc gttataacta cctgattaat
    42781 cagctgaata ttaagtcagc cctgggtacg ttgaacgagc aggatctgct ggcactgaac
    42841 aatgcgctga gcaaaccggt ttccactaat ccggaaaacg ttgccccgca aacgccggaa
    42901 cagaatgcta ttgctgatgg ttatgcgcct gatagcccgg cccccgtcgt tcagcaaaca
    42961 tccgcacgca ctaccaccag taacggtcat aaccctttcc gtaactgatg acgacgacgg
    43021 ggcttcggcc ccgtctgaac gtaaggcaac gtaaagatac gggttatctg ccgcattctt
    43081 cccccttctc gcttcaattt cgaccagcca tcctctattc tgatgggtat ttaccactgg
    43141 tcccggaaga caaaaatgaa acggacaaaa tccatacgcc acgcatcgtt ccgcaaaaac
    43201 tggagcgcac gccatctgac accagtcgct ctcgcggttg ccactgtttt tatgctggct
    43261 ggctgtgaaa agagtgatga aacagtgtct ctctatcaaa atgctgacga ctgttcagct
    43321 gcaaacccag gcaaaagcgc cgaatgtacc accgcgtaca acaatgcgct gaaagaagcc
    43381 gaacgtactg cgccgaaata cgccacccgt gaagactgtg ttgctgaatt tggtgaaggt
    43441 cagtgccagc aggcaccagc ccaggctggc atggcaccag aaaaccaggc gcaggcccag
    43501 caatccagcg ggagtttctg gatgccgctg atggccggtt acatgatggg gcgtctgatg
    43561 ggcggcggcg cgggatttgc acagcagccg ctgttctcct cgaaaaaccc agccagtccg
    43621 gcttacggta aatataccga cgcgacgggt aaaaactatg gcgcagccca gccaggccgc
    43681 accatgaccg taccgaagac ggcaatggca ccaaaaccgg cgaccaccac taccgttacc
    43741 cgtggcggtt ttggtgaatc tgttgccaaa caaagcacta tgcagcgtag cgcaaccggc
    43801 acctcttctc gttcaatggg tggctgatac cgatggaaag agtcagtatt accgagcgcc
    43861 cggactggcg tgagaaagcc cacgaatacg gtttcaattt tcacaccatg tacggcgagc
    43921 cgtactggtg tgaagatgct tactacaagt tgaccctcgc ccaggttgaa aagctggaag
    43981 aagtcaccgc cgaactgcac cagatgtgcc tgaaagtggt ggaaaaagtg atcgccagcg
    44041 atgagctgat gaccaaattc cgcattccaa aacacacctg gagttttgtg cgtcaatcat
    44101 ggctgacgca ccagccatcg ctttattcgc gtcttgatct ggcgtgggat ggcactggcg
    44161 aacctaaact tctggaaaat aacgccgata cgccaacgtc actatacgag gcggcgttct
    44221 ttcagtggat ctggctggaa gatcagctta acgccggtaa cttgccggag ggcagcgacc
    44281 agtttaacag tctgcaagaa aaactgatcg atcgcttcgt tgagctgcgt gaacagtatg
    44341 gcttccagtt gctgcatctc acctgctgtc gcgacacggt ggaagatcgc ggaaccattc
    44401 agtatttgca ggactgcgcg acggaagctg aaattgctac tgagttcctc tacatcgatg
    44461 atatcgggtt aggtgaaaaa ggtcagttca cggatttaca ggatcaggta atttccaacc
    44521 tgttcaagct gtatccgtgg gaatttatgt tgcgtgagat gttctcaacc aagctggagg
    44581 atgcaggcgt acgctggctg gaaccggcgt ggaagagcat tatctccaac aaggcacttc
    44641 taccgctact gtgggagatg ttcccgaatc acccgaacct gctgcccgct tattttgcgg
    44701 aagatgatca tccgcaaatg gaaaaatatg tggttaaacc gatcttctcc cgtgaaggcg
    44761 caaacgtgtc gatcattgag aacggcaaaa ccattgaagc agcggaaggt ccgtatggcg
    44821 aagaagggat gattgttcag caattccacc cgttaccgaa attcggcgac agctatatgc
    44881 tgattggtag ctggctggtg aacgatcaac ccgccggaat tggcattcgt gaagaccgtg
    44941 cattgatcac ccaggatatg tctcggtttt atccacatat ttttgttgag taatcaatga
    45001 tgccggatgg cattcgccat ccggtaattg ttaacctatc tgcaccgaca gcatacttaa
    45061 actgcccatt tctataccat cgaccggaat ggtaattggc tcctgcccat cccacgcacc
    45121 taacacatac aacaacggca gatagtgctc tggcgttggg ttcgataacg cgccaccttc
    45181 atggtcgaga taattcacca gaggatgctg ctctaccggc ccttgccacg tcagattcgc
    45241 tttcacatac tcattaaacg acgtcgccca cggatacggt gaactatcac cgtgccactt
    45301 cactgtgcgc aggttatgca ccacgttacc gctggcgacc aacattattc cttcatcacg
    45361 cagcgccgcg agtttgcgcc ccatttcgaa atgccaggcg gcaggtttgc tactgtcgat
    45421 actcaactgc accatcggga tatcggcgtc aggatacatc ttaatcagca cgccccagga
    45481 accgtggtca aagccccagg cttctttatc cagcgtcacc gggatcggcg ctaacagctc
    45541 aaccagacgc tgtgccagcg caggcgaacc cggagcagga taatgcgtat cgtacagcgc
    45601 ctgcgggaag ccaccaaagt catgaatcgt gggcggcgtc tccatcgcgg tcactcctgt
    45661 tccacgggta aaccagtgag ccgaaaccac cacaatcgct tgcgggcgtg gcaatgtcat
    45721 ccccaacttc tgccagctgc gggtatacaa attatcttcc agcacgttca tcggactacc
    45781 gtgacctaaa aacaatgctg gcatacgtgt tgaagacatg atgatatcct taactaaagg
    45841 tgtcattttg atatcctcac aatacgcttg ttcggcggag taagaacccg gataacaatg
    45901 atgatgatca tcagttattt tgacgatctg cctgaaggtg aagatttata aggagttgtc
    45961 gatgtcagta cctctcattc tgaccatact ggcgggggca gccacgttta ttggcgcgtt
    46021 tctcggcgtt ctcgggcaaa aaccctcgaa ccgcttactg gcgttttcgc taggatttgc
    46081 ggcggggatc atgttgctca tctcattaat ggaaatgctt cctgccgcac tagcagctga
    46141 aggaatgtcg cctgtgttgg gttatggaat gtttatcttc ggtctgcttg gctattttgg
    46201 cctggaccgc atgttgccac atgcgcatcc tcaggattta atgcaaaaat cggtgcagcc
    46261 gttgccaaaa tcgatcaagc gcacagccat tctgctcact ctcggcatca gtctgcataa
    46321 cttcccggaa gggattgcca cctttgtcac ggcgagcagc aacctggagc tgggatttgg
    46381 catcgcactg gccgtcgcgt tgcacaatat ccctgaaggt ctggcagtgg caggcccggt
    46441 ttatgcggca acggggtcta aacgcaccgc gattctgtgg gcggggattt ctggactagc
    46501 agaaattctt ggtggtgtgc tggcgtggtt aatcctcggt agcatgattt ccccggtggt
    46561 catggcggca atcatggcgg cggttgcagg aattatggtg gcgctctcgg ttgatgaatt
    46621 aatgccgctc gccaaagaga ttgaccctaa taataacccc agctatggcg tactatgtgg
    46681 aatgtcagtg atgggattca gtttagtgct gctacaaacg gcgggaattg gttaaaaaca
    46741 acatcagaag ttgtccagcg catggacaac ttctgccagc ttcacgaaag ccttattcta
    46801 ccttttcagc tgtctcttct ctcgtactgt ttaagtattt gttccgcgct tcaagcagtg
    46861 cgtctctgtc ctggcgaaac gcccgacaat aacgtttcaa atgcaggaaa taacccacca
    46921 caaccagagc aatcaataca atccagtacc aggcgataaa catcctttta cccttaatat
    46981 ttaacaatac taaacattta gcgcataaat ttcacatatc ctttttcgga tatattcata
    47041 tggtcgcata ataacggaac aattatatga ttttgatcat aatgaaaaag aatgcctgat
    47101 attaatgaaa ttttctcata gaaagagaag cggaattaac atcttgcata gcagggaaaa
    47161 ataaaaaccg aagcctgcta caacttcggt ttcttgatta aggcagtaat ttaagcagcg
    47221 gttttcagct ggctttacgc tcatgtgcct gacggtatgc caccaggtct tcaatagtca
    47281 cgagcgccat attgtgttta ttggcaaact caatgcactc tggtgcacgc gccatcgtgc
    47341 catcgtcatt agtcagctca cacagcacac cagccggttt aaagcctgcc agcgtcatca
    47401 aatcaatagt cgcttcagta tgaccgccac gcgtcagtac accacctgcc tgcgcgcgaa
    47461 gtgggaaaac gtggcccggg cgattcagat cagaaggttt tgcgccatcg gcaatcgctg
    47521 cgcgaacggt cgtaatacga tcagcggcag aaacaccggt agtcacacct tcagccgctt
    47581 caatggtcac ggtaaaaccg gtgccatagg cgctggtgtt attttctacc atcattggca
    47641 gatcaagttg tttacggcga tcttcagtga tgcacaggca aacaataccg ctaccgtggc
    47701 gaatggtcag cgccatctgc tcaacggtca tggtttctgc cgggaagatc atatcgcctt
    47761 cgttttcacg gtcttcatca tcaagcacca ttacaccgcg tccttcacgc agcgcagcca
    47821 gtgcattttc aacacgttcg aaaggcgtac caaaagagga aagtagcgtc tgattcatgg
    47881 taaaaaaacc tcactaaaat tatggttacc agaatcaggg cagtcttagg agtggcggca
    47941 tatagccaaa ataacgtgag cgggtccatg cccgacagaa tcgttactct ctcccatccg
    48001 gactctaacc gtcggccccg gaattacacc ggatctgctg tcctttgagt tcgcactcaa
    48061 agcgctcgcg ggctttcaac caagttgatt taccgccggt ggggaatttc gccccgccct
    48121 gagaataagc ggattcacta taacgctaat gattagcggc agcaacgcat agcttcacat
    48181 aattctggtt tatgacttac ccttatcgca ctacaatggc actcaacacc tatcagtaca
    48241 gggaaactac aatgattgac ccgaaaaaaa ttgagcaaat cgctcgccag gttcacgaat
    48301 caatgcctaa aggaatcagg gagttcgggg aagatgtgga gaaaaaaatc cgccaaaccc
    48361 tacaagcgca gctgacgcgc ctcgaccttg taagccgtga agagttcgac gtccaaacgc
    48421 aagtcctgtt acggactcgt gagaaactgg cgctgcttga acaaagaatc agcgaactgg
    48481 aaaatcgcag tactgagatc aaaaagcagc cagatccaga gactctcccc ccaacgctgt
    48541 aaacatttgt ctttaatgca ctgtaaatta tgaattaatc ctctaattta cagtgttttc
    48601 tattacatta tcgcttaatc ccattcagag tatcatcac
//
LOCUS       DAJOSY010000020        48238 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJOSY010000020 DAJOSY010000000
VERSION     DAJOSY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.020"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            161..1378
                     /gene="alaA"
                     /locus_tag="OHN27_001265"
     CDS             161..1378
                     /gene="alaA"
                     /locus_tag="OHN27_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCQ2285746.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1462..2061
                     /gene="yfbR"
                     /locus_tag="OHN27_001266"
     CDS             1462..2061
                     /gene="yfbR"
                     /locus_tag="OHN27_001266"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCQ2285747.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLESRRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2120..3952)
                     /locus_tag="OHN27_001267"
     CDS             complement(2120..3952)
                     /locus_tag="OHN27_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCQ2285748.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4039..4689)
                     /gene="hxpA"
                     /locus_tag="OHN27_001268"
     CDS             complement(4039..4689)
                     /gene="hxpA"
                     /locus_tag="OHN27_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCQ2285749.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4700..5194)
                     /locus_tag="OHN27_001269"
     CDS             complement(4700..5194)
                     /locus_tag="OHN27_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCQ2285750.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5277..5732)
                     /locus_tag="OHN27_001270"
     CDS             complement(5277..5732)
                     /locus_tag="OHN27_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCQ2285751.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6070..7272
                     /gene="ackA"
                     /locus_tag="OHN27_001271"
     CDS             6070..7272
                     /gene="ackA"
                     /locus_tag="OHN27_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCQ2285752.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7347..9491
                     /gene="pta"
                     /locus_tag="OHN27_001272"
     CDS             7347..9491
                     /gene="pta"
                     /locus_tag="OHN27_001272"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCQ2285753.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9681..11201
                     /gene="yfcC"
                     /locus_tag="OHN27_001273"
     CDS             9681..11201
                     /gene="yfcC"
                     /locus_tag="OHN27_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCQ2285754.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11234..11776)
                     /gene="yfcD"
                     /locus_tag="OHN27_001274"
     CDS             complement(11234..11776)
                     /gene="yfcD"
                     /locus_tag="OHN27_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCQ2285755.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11834..12385)
                     /gene="yfcE"
                     /locus_tag="OHN27_001275"
     CDS             complement(11834..12385)
                     /gene="yfcE"
                     /locus_tag="OHN27_001275"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCQ2285756.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12441..13085)
                     /gene="yfcF"
                     /locus_tag="OHN27_001276"
     CDS             complement(12441..13085)
                     /gene="yfcF"
                     /locus_tag="OHN27_001276"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCQ2285757.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIVEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13221..13868
                     /gene="yfcG"
                     /locus_tag="OHN27_001277"
     CDS             13221..13868
                     /gene="yfcG"
                     /locus_tag="OHN27_001277"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCQ2285758.1"
                     /translation="MIDLYFAPTPNGHKITLFLEETELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13925..14287
                     /gene="folX"
                     /locus_tag="OHN27_001278"
     CDS             13925..14287
                     /gene="folX"
                     /locus_tag="OHN27_001278"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCQ2285759.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14308..15201
                     /locus_tag="OHN27_001279"
     CDS             14308..15201
                     /locus_tag="OHN27_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCQ2285760.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15249..16139)
                     /gene="rpnB"
                     /locus_tag="OHN27_001280"
                     /pseudo
     CDS             complement(15249..16139)
                     /gene="rpnB"
                     /locus_tag="OHN27_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
     gene            complement(16336..17109)
                     /gene="hisP"
                     /locus_tag="OHN27_001281"
     CDS             complement(16336..17109)
                     /gene="hisP"
                     /locus_tag="OHN27_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCQ2285761.1"
                     /translation="MSENKLNVIDLHKRYGKHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17117..17833)
                     /locus_tag="OHN27_001282"
     CDS             complement(17117..17833)
                     /locus_tag="OHN27_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2285762.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17830..18516)
                     /gene="hisQ"
                     /locus_tag="OHN27_001283"
     CDS             complement(17830..18516)
                     /gene="hisQ"
                     /locus_tag="OHN27_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCQ2285763.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18606..19388)
                     /gene="hisJ"
                     /locus_tag="OHN27_001284"
     CDS             complement(18606..19388)
                     /gene="hisJ"
                     /locus_tag="OHN27_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCQ2285764.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19609..20391)
                     /gene="argT"
                     /locus_tag="OHN27_001285"
     CDS             complement(19609..20391)
                     /gene="argT"
                     /locus_tag="OHN27_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCQ2285765.1"
                     /translation="MKKSILALSLLVGLSTAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20657..21226)
                     /gene="ubiX"
                     /locus_tag="OHN27_001286"
     CDS             complement(20657..21226)
                     /gene="ubiX"
                     /locus_tag="OHN27_001286"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCQ2285766.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSVIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21321..22838)
                     /gene="purF"
                     /locus_tag="OHN27_001287"
     CDS             complement(21321..22838)
                     /gene="purF"
                     /locus_tag="OHN27_001287"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCQ2285767.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22875..23363)
                     /gene="cvpA"
                     /locus_tag="OHN27_001288"
     CDS             complement(22875..23363)
                     /gene="cvpA"
                     /locus_tag="OHN27_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCQ2285768.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23622..24284)
                     /gene="dedD"
                     /locus_tag="OHN27_001289"
     CDS             complement(23622..24284)
                     /gene="dedD"
                     /locus_tag="OHN27_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCQ2285769.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24274..25542)
                     /gene="folC"
                     /locus_tag="OHN27_001290"
     CDS             complement(24274..25542)
                     /gene="folC"
                     /locus_tag="OHN27_001290"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCQ2285770.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSLVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25612..26526)
                     /gene="accD"
                     /locus_tag="OHN27_001291"
     CDS             complement(25612..26526)
                     /gene="accD"
                     /locus_tag="OHN27_001291"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCQ2285771.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26682..27341)
                     /locus_tag="OHN27_001292"
     CDS             complement(26682..27341)
                     /locus_tag="OHN27_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2285772.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27424..28236)
                     /gene="truA"
                     /locus_tag="OHN27_001293"
     CDS             complement(27424..28236)
                     /gene="truA"
                     /locus_tag="OHN27_001293"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCQ2285773.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTALRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28236..29249)
                     /locus_tag="OHN27_001294"
     CDS             complement(28236..29249)
                     /locus_tag="OHN27_001294"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCQ2285774.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29315..30451)
                     /gene="pdxB"
                     /locus_tag="OHN27_001295"
     CDS             complement(29315..30451)
                     /gene="pdxB"
                     /locus_tag="OHN27_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCQ2285775.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30550..31545
                     /gene="flk"
                     /locus_tag="OHN27_001296"
     CDS             30550..31545
                     /gene="flk"
                     /locus_tag="OHN27_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCQ2285776.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31542..32720)
                     /locus_tag="OHN27_001297"
     CDS             complement(31542..32720)
                     /locus_tag="OHN27_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2285777.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPVLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(32985..34205)
                     /gene="fabB"
                     /locus_tag="OHN27_001298"
     CDS             complement(32985..34205)
                     /gene="fabB"
                     /locus_tag="OHN27_001298"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCQ2285778.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34364..36370
                     /gene="mnmC"
                     /locus_tag="OHN27_001299"
     CDS             34364..36370
                     /gene="mnmC"
                     /locus_tag="OHN27_001299"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCQ2285779.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQARLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36491..36769)
                     /locus_tag="OHN27_001300"
     CDS             complement(36491..36769)
                     /locus_tag="OHN27_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCQ2285780.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36803..37351)
                     /gene="epmC"
                     /locus_tag="OHN27_001301"
     CDS             complement(36803..37351)
                     /gene="epmC"
                     /locus_tag="OHN27_001301"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCQ2285781.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37351..38160)
                     /locus_tag="OHN27_001302"
     CDS             complement(37351..38160)
                     /locus_tag="OHN27_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCQ2285782.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38160..38984)
                     /gene="mepA"
                     /locus_tag="OHN27_001303"
     CDS             complement(38160..38984)
                     /gene="mepA"
                     /locus_tag="OHN27_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCQ2285783.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCFDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(38988..40073)
                     /gene="aroC"
                     /locus_tag="OHN27_001304"
     CDS             complement(38988..40073)
                     /gene="aroC"
                     /locus_tag="OHN27_001304"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCQ2285784.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40108..41040)
                     /gene="prmB"
                     /locus_tag="OHN27_001305"
     CDS             complement(40108..41040)
                     /gene="prmB"
                     /locus_tag="OHN27_001305"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCQ2285785.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41206..41757
                     /gene="smrB"
                     /locus_tag="OHN27_001306"
     CDS             41206..41757
                     /gene="smrB"
                     /locus_tag="OHN27_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCQ2285786.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41829..42686)
                     /locus_tag="OHN27_001307"
     CDS             complement(41829..42686)
                     /locus_tag="OHN27_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCQ2285787.1"
                     /translation="MKILRYVFYFLLLICLSTGVQAAKKIATLYIPAGSMGVLTGFQF
                     NLSILTPEGAQYGVYQSRSVAILNVPLVSWTGQGTAPELHLEDARTILPTSRCPGLEE
                     YDARTPQTTWACVEMKVTVFYDGDMHGCPWIVSSYVKSEDATEAIYTGNNVPYIGPTK
                     VSSDCSAIPLDPYDVSWNENTVVHNKIVRMQSTGAVIEQTLPTYLMEGGKLCDGSKYD
                     ERGAYCRFVAQQMTFSTSGCDDAKVTVTPEPQPITSRQLHDMKLRVDTTAQQPIDATC
                     RFTYILNMY"
     gene            complement(42688..43227)
                     /locus_tag="OHN27_001308"
     CDS             complement(42688..43227)
                     /locus_tag="OHN27_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2285788.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLIVAGQSVHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGEFTASLTATFEYI"
     gene            complement(43224..43712)
                     /locus_tag="OHN27_001309"
     CDS             complement(43224..43712)
                     /locus_tag="OHN27_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2285789.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQEVDYELSCESDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            complement(43709..44218)
                     /locus_tag="OHN27_001310"
     CDS             complement(43709..44218)
                     /locus_tag="OHN27_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2285790.1"
                     /translation="MSHKFLAGAGLLVLAGWGVSGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVEFGNVVINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44234..44986)
                     /locus_tag="OHN27_001311"
     CDS             complement(44234..44986)
                     /locus_tag="OHN27_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCQ2285791.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNNCQVDEEQSRKG"
     gene            complement(45006..47648)
                     /gene="yfcU"
                     /locus_tag="OHN27_001312"
     CDS             complement(45006..47648)
                     /gene="yfcU"
                     /locus_tag="OHN27_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCQ2285792.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYLEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGVEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVTSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47730..>48238)
                     /locus_tag="OHN27_001313"
     CDS             complement(47730..>48238)
                     /locus_tag="OHN27_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2285793.1"
                     /translation="STATIAAGNNGTARFYGTIEDSVCSIVPDDHKLEVDMGDIGAEK
                     LKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYYTIFNTDTGAAFNNV
                     SLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPDLGNFEAN
                     TTFQITYL"
BASE COUNT        11718 a        13067 c        12095 g        11358 t
ORIGIN      
        1 ttattttgtc attattaagt tattaacagc acaatcgagc cttcccctct ggcaaaatct
       61 tattctgcag accttcaaaa caccgtcctg ggggagtaca ttgttctaag ctgacttcca
      121 cggcagggag tggcgataac agcaaaaaag gtcaagattc atgtccccca ttgaaaaatc
      181 cagcaaatta gagaatgtct gttatgacat ccgtggtccg gtgctgaaag aagcaaaacg
      241 cctggaagaa gaaggtaaca aggtactgaa actgaacatc ggcaacccag ccccgttcgg
      301 ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc aacctgccta ccgctcaagg
      361 gtattgcgat tccaaagggc tttactccgc gcgtaaagcc atcatgcagc actaccaggc
      421 tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc ggcaatggtg tatcggagct
      481 tatcgttcag gcaatgcagg cattgctgaa cagcggggac gaaatgttgg ttcctgcacc
      541 agattaccca ctatggaccg cggcggtttc gctttccagt ggtaaagcgg tgcattatct
      601 ttgcgatgaa tcctctgact ggttcccgga cctcgatgat attcgcgcta aaattacgcc
      661 tcgtacgcgt gggatcgtta ttatcaaccc aaataaccca accggcgcgg tatattccaa
      721 agagctttta atggagattg tggagattgc acgtcagcat aatctcatta tcttcgccga
      781 tgaaatttat gacaaaattc tttacgacga cgctgagcat cactcaattg cgccgctggc
      841 acctgacctg ctgaccatta cctttaacgg actgtcgaaa acgtaccgcg ttgcaggctt
      901 ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac gccaaaggct acatcgaagg
      961 tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt cctgcgcaac acgccattca
     1021 gaccgcgctg ggtggttacc agagcatcag tgaatttatt acccctggcg gtcgtcttta
     1081 tgagcagcgt aaccgcgcgt gggaactgat caacgatatt ccgggcgttt cctgcgtgaa
     1141 acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc aaacgcttta acattcacga
     1201 cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa gttctgttgg tgcaagggac
     1261 ggcattcaac tggccgtggc cggatcactt ccgcattgtc acgctaccgc gtgtcgatga
     1321 tatcgagctg tctttgagca agttcgcgcg tttcctttct ggttatcatc agctgtaatc
     1381 ttaatttcac tgccggagat tgcatccggc agtgttatcc cgccacaatg acctgatgat
     1441 gtcatcatac gtaaggtcac tatgaaacag agccattttt ttgcccatct ctcccgcctg
     1501 aaactcatta accgctggcc gctcatgcgc aacgtgcgga cggaaaatgt gtccgaacac
     1561 agtttgcagg tagcgatggt cgcccatgcg ctggcagcta tcaaaaatcg gaaatttggc
     1621 ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt accacgatgc ctcagaagtg
     1681 ctcaccggcg atctccctac tccggtgaaa tacttcaatt cgcaaatcgc tcaggagtac
     1741 aaggctattg aaaaaatcgc tcagcaaaaa ctggtcgata tggttccgga agagctgcgg
     1801 gatatctttg cgccgttaat tgacgagcat gcatatagcg atgaagaaaa atcgctggtg
     1861 aaacaggcag atgcactgtg tgcatatctg aaatgtctgg aagaactcgc ggccggaaat
     1921 aatgaattct tgctggcaaa aacgcgactg gaagcgacgc ttgaatcgcg tcgcagccag
     1981 gagatggact acttcatgga agtatttgtt cccagcttcc atctttcgct cgatgagatt
     2041 agccaggatt caccgctgta agcagccgga gtctgcgtcg catcaggcaa taagcgccgg
     2101 atgcgacatc aggctcttgt caaaacggaa acagcatcgg gatcatcacc acacaaaccg
     2161 ccatcacgat aatggtgaac ggtaccccca acttcacaaa gtcactgaag ctgtaatttc
     2221 ccggacctaa aaccagtgtg ttaacaggtg aagaaaccgg tgtcataaag gcggcggatg
     2281 ctgccatcgc cacgaccatc gcgaatggat aaggcgacac ccccatcgtt ttggcagcag
     2341 ccagcgcaat cggagccatc aacaccgccg tcgcggtatt agagataaat agcccaataa
     2401 ccgccgacaa gacaaacaaa caccccagca tcatatgtgg cccgtaacca ccgccaatgt
     2461 ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt ctgtaatgcc acagcaaacg
     2521 gcatcatccc aacgatcaaa ataatgctcg gccagtgaat ggatttatag gcgctttcag
     2581 catctataca gcggaatttc cccatcagca ggcaggcgat gatagcggca acaggattag
     2641 gaatttcatc tgtcagcatt aacgccacca ttagcaccag acagaaaatg gcatggggtg
     2701 cctggctgtg cgcgggcgat gcttcactca cctcttccgg taagttcagc gctacgaagt
     2761 cgcggccctg tttggccagc ataccgatca gtttccagtt acccacaacc aggatgatat
     2821 cgcccagcag cagaggctca tccgccagcg aaccttccag cgccacgcca ttgcgcttta
     2881 gccccaccac attcagtccg tagcgggtac gaaaaccaat ttcgcgcacc gatttaccaa
     2941 tcagttctga ctcaggaatt aatgaaatct ctgccatgcc cacatcaagg gcctggtcag
     3001 aaaaatactc gccgcgcagt accatcggct ccagcaattg ctcactacaa aattgccgga
     3061 gatcgacatc agccgcagac atatcaataa gcaaaacgtc acgcgcgcga aattcagaaa
     3121 ccccattaac gttcacgata acgcgacgaa aacgccgcca gcgttcaaca ccgatgacgt
     3181 tagcgccata acgctcacgt aatttgagat catccagccg ttgaccaatc attggcgatc
     3241 cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg atattcacgg ataagatcgc
     3301 gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg ggtatcccct ttcagcatga
     3361 aacgcatcac taacatatac aaaataccca gcaccagcac gaccaggcca ataggtgtta
     3421 cgctaaagaa actgaagccg tgatagcctt cacgcagcaa ttcactgttg actaccaggt
     3481 tcggcggcgt cgccaccagc gtcatcatgc cgcttatcag cccggcaaaa ctcaacggca
     3541 tcatcagacg tgacggcgaa gtttgcatac gcatggcaac gcttaacacc acgggaataa
     3601 agatagcgac aacgccggtt gaactcataa acgcgccaag ccccgcgacg gtcagcatca
     3661 acaaaaccaa cattttgatt tcactgttgc ccgcaacttt gaccagccat gttcccatta
     3721 cggtggcaac accggtacgg accaaaccat cgccaataat aaacaaggcg gcaatcagga
     3781 caacgttagg atcagaaaag ccggaaaata cttcggggac tgtcagcgtt ccgcttaatg
     3841 caaacgcgac aataacaaac aaagcgaccg catccatacg cactctgccc gtcgcaaaca
     3901 agacaatggc aacggccagt aatgaaagaa cccaaatcaa ttcaccgttc acaacttatc
     3961 cttgttaatt gagggagatg tcttgattct gccataaaaa agccccgacg agacggggct
     4021 aaatcatgat caggtgtttc actgaataat aacatcgcca tttggctgtt tggtcacagt
     4081 aatttgctcc agactgtgga ggaccaaatc gacctcattc aggcgcgggg tatctgccgg
     4141 agcgttaacc gcaatgacat gacaacccgc cgccaggcca gaaagcacgc cagcgggagc
     4201 atcttccacc accacacact cctgcggcgc aagccccagc agctgcgcgc ctaacagata
     4261 cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg gttacaaaca cctctggtgc
     4321 gggaagccca gctattttat ggcgcgctcg cgctaccggc atggagccag aagtcacaat
     4381 ggcccacgga atacctgctt tattcaaatg actgagtaag gcgattgccc ccggaagcgc
     4441 ggtaatacct tcggtttccg tggcctcgat gtgctccaga cgcgtaaact cggcggcaat
     4501 atcagcctcg gatttgcccg ccataaaatg gcgcagagag gtgatcgcct gtttaccgtg
     4561 aatgaaagcc agcacctctt ccggcgctaa cccatgacgt ctggcccagt tgctccacgc
     4621 ccgttctacc gcaggcaggg aatccaccag cgttccatca agatcaaaca gaaaaccttt
     4681 gcaccgcacg cgggcctcct caggcattga taatttgatt aatttcgttg gcgctcaaat
     4741 ggtactgacg cgggcaggca tgccacacat taagcatgcg ctgatatttt tcccacattg
     4801 gcgtctgggc gttaaaaccg tgagttccgg cgtcaaaatg ggtatagcgc ccttccacat
     4861 taaccataaa gcggacataa ccgaggtaac gtgcttcagt ggcggcgtca aagccgagga
     4921 aggtgacacg acgttcatcg atggattgct ggtcctgcaa attagaccag gaaacatgca
     4981 acgcatgata catctccata atgtcgatga tagtgcggca ggtttcttct ttcagctcgc
     5041 caaactcgcg atccaattca cgcatctgta atccgtaacc acgctcaata attgtttgca
     5101 ggcgacggta acgttcagca tttgccggat cgagcatagt catcatcttg tactgattag
     5161 acaaaataag acgttgcgcg ttggtcattt ccattgttga ctcctgtatc actctactac
     5221 ggtgaaaaaa aagaaggctg agtatgcctt cttttatatg cgtaatcagg ggtcaattac
     5281 aaatcatcaa ggaaagtttt atccagttgt ttgaaggcgc gcttaagcgt gtcagctaat
     5341 gcctggtaat caggcttgcc ttcaacgggt gccaacacct gtccagactc ctgcaattta
     5401 ccgcgaactt cataaaacca gttaaggatt gcagggggta atggcgttac agaacgcttg
     5461 cccagccacc acaatccctg catgggtaaa cttaaggcga acagcgcagt ggcaactgcc
     5521 ggcccaagct gaccgcccag ggcaatctgc cagcagagag taaatacggc gatcggcggc
     5581 ataaaacgga tcgcataacg cgtcatcttg ataacgcgat tttcgacaaa gaccggggca
     5641 aggcgttttt ccagcggcca cgtctttgag taatgctgtc cccggcgaaa caagctaaaa
     5701 aaattaacag aacgattatc cggcgttgac atgcttcacc tcaacttcac atataaagat
     5761 tcaaaaattt gtgcaaattc acaactcagc gggacaacgt tcaaaacatt ttgtcttcca
     5821 tacccactat caggtatcct ttagcagcct gaaggcctaa gtagtacata ttcattgagt
     5881 cgtcaaattc atatacatta tgccattggc tgaaaattac gcaaaatggc atagactcaa
     5941 gatatttctt ccatcatgca aaaaaaattt gcagtgcatg atgttaatca taaatgtcgg
     6001 tgtcatcatg cgctacgctc tatggctccc tgacgttttt ttagccacgt atcaattata
     6061 ggtacttcca tgtcgagtaa gttagtactg gttctgaact gcggtagttc ttcactgaaa
     6121 tttgccatca tcgatgcagt aaatggtgaa gagtaccttt ctggtttagc cgaatgtttc
     6181 cacctgcccg aagcacgtat caaatggaaa atggacggca ataaacagga agcggcttta
     6241 ggtgcaggcg ccgctcacag cgaagcgctc aactttatcg ttaatactat tctggcacaa
     6301 aaaccagaac tgtctgcgca gctgactgct atcggtcacc gtatcgtaca cggcggcgaa
     6361 aagtatacca gctccgtagt gatcgatgag tctgttattc agggtatcaa agatgcagct
     6421 tcttttgcac cgctgcacaa cccggctcac ctgatcggta tcgaagaagc tctgaaatct
     6481 ttcccacagc tgaaagacaa aaacgttgct gtatttgaca ccgcgttcca ccagactatg
     6541 ccggaagagt cttacctcta cgccctgcct tacaacctgt acaaagagca cggcatccgt
     6601 cgttacggcg cgcacggcac cagccacttc tatgtaaccc aggaagcggc aaaaatgctg
     6661 aacaaaccgg tagaagaact gaacatcatc acctgccacc tgggcaacgg tggttccgtt
     6721 tctgctatcc gcaacggtaa atgcgttgac acctctatgg gcctgacccc gctggaaggt
     6781 ctggtcatgg gtacccgttc tggtgatatc gatccggcga tcatcttcca cctgcacgac
     6841 accctgggca tgagcgttga cgcaatcaac aaactgctga ccaaagagtc tggcctgctg
     6901 ggtctgaccg aagtgaccag cgactgccgc tatgttgaag acaactacgc gacgaaagaa
     6961 gacgcgaagc gcgcaatgga cgtttactgc caccgcctgg cgaaatacat cggtgcctac
     7021 actgcgctga tggatggtcg tctggacgct gttgtattca ctggtggtat cggtgaaaat
     7081 gccgcaatgg ttcgtgaact gtctctgggc aaactgggcg tgctgggctt tgaagttgat
     7141 catgaacgca acctggctgc acgtttcggc aaatctggtt tcatcaacaa agaaggtacc
     7201 cgtcctgcgg tggttatccc aaccaacgaa gaactggtta tcgcgcaaga cgcgagccgc
     7261 ctgactgcct gatttcacac cgccagctca gctggcggtg ctgttttgta acccgccaaa
     7321 tcggcggtaa cgaaagagga taaaccgtgt cccgtattat tatgctgatc cctaccggaa
     7381 ccagcgtcgg tctgaccagc gtcagccttg gcgtgatccg tgcaatggaa cgcaaaggcg
     7441 ttcgtctgag cgttttcaaa cctatcgctc agccgcgtac cggtggcgat gcgcccgatc
     7501 agactacgac tatcgtgcgt gcgaactctt ccaccacgac ggccgctgaa ccgctgaaaa
     7561 tgagctacgt tgaaggtctg ctttccagca atcagaaaga tgtgctgatg gaagagatcg
     7621 tcgcaaacta ccacgctaac accaaagacg ctgaagtcgt tctggttgaa ggtctggtcc
     7681 cgacacgtaa gcaccagttt gcccagtctc tgaactacga aatcgctaaa acgctgaatg
     7741 cggaaatcgt cttcgttatg tctcagggca ctgacacccc ggaacagctg aaagagcgta
     7801 tcgaactgac ccgcaacagc ttcggcggtg ccaaaaacac caacatcacc ggcgttatcg
     7861 ttaacaaact gaacgcaccg gttgatgaac agggtcgtac tcgcccggat ctgtccgaga
     7921 ttttcgacga ctcttccaaa gctaaagtaa acaatgttga tccggcgaag ctgcaagaat
     7981 ccagcccgct gccggttctc ggcgctgtgc cgtggagctt tgacctgatc gcgactcgtg
     8041 cgatcgatat ggctcgccac ctgaatgcga ccatcatcaa cgaaggcgac atcaatactc
     8101 gccgcgttaa atccgtcact ttctgcgcac gcagcattcc gcacatgctg gagcacttcc
     8161 gtgccggttc tctgctggtg acttccgcag accgtcctga cgtgctggtg gccgcttgcc
     8221 tggcagccat gaacggcgta gaaatcggtg ccctgctgct gactggcggt tacgaaatgg
     8281 acgcgcgcat ttctaaactg tgcgaacgtg ctttcgctac cggcctgccg gtatttatgg
     8341 tgaacaccaa cacctggcag acctctctga gcctgcagag cttcaacctg gaagttccgg
     8401 ttgacgatca cgaacgtatc gagaaagttc aggaatacgt tgctaactac atcaacgctg
     8461 actggatcga atctctgact gccacttctg agcgcagccg tcgtctgtct ccgcctgcgt
     8521 tccgttatca gctgactgaa cttgcgcgca aagcgggcaa acgtatcgta ctgccggaag
     8581 gtgacgaacc gcgtaccgtt aaagcagccg ctatctgtgc tgaacgtggt atcgcaactt
     8641 gcgtactgct gggtaatccg gcagagatca accgtgttgc agcgtctcag ggtgtagaac
     8701 tgggtgcagg gattgaaatc gttgatccag aagtggttcg cgaaagctat gttggtcgtc
     8761 tggtcgaact gcgtaagaac aaaggcatga ccgaaaccgt tgcccgcgaa cagctggaag
     8821 acaacgtggt gctcggtacg ctgatgctgg aacaggatga agttgatggt ctggtttccg
     8881 gtgctgttca cactaccgca aacaccatcc gtccgccgct gcagctgatc aaaactgcac
     8941 cgggcagctc cctggtatct tccgtgttct tcatgctgct gccggaacag gtttacgttt
     9001 acggtgactg tgcgatcaac ccggatccga ccgctgaaca gctggcagaa atcgcgattc
     9061 agtccgctga ttccgctgcg gccttcggta tcgaaccgcg cgttgctatg ctctcctact
     9121 ccaccggtac ttctggtgca ggtagcgacg tagaaaaagt tcgcgaagca actcgtctgg
     9181 cgcaggaaaa acgtcctgac ctgatgatcg acggtccgct gcagtacgac gctgcggtaa
     9241 tggctgacgt tgcgaaatcc aaagcgccga actctccggt tgcaggtcgc gctaccgtgt
     9301 tcatcttccc ggatctgaac accggtaaca ccacctacaa agcggtacag cgttctgccg
     9361 acctgatctc catcgggccg atgctgcagg gtatgcgcaa gccggttaac gacctgtccc
     9421 gtggcgcact ggttgacgat atcgtctaca ccatcgcgct gactgcgatt cagtctgcac
     9481 agcagcagta atctcgtcat catccgcagc tttgcgctgc ggatatctga accggaaata
     9541 atcactattt ccggtttttt attctcttaa tctgcattaa tcctttctga ttatcttgct
     9601 taactgcgct gcatcaatga attgcgccat ttcactttgc atacttacca ctttgttttg
     9661 tgcaagggaa tatttgcgct atgtccgcaa tcactgaatc caaaccaaca agaagatggg
     9721 caatgcccga tacgttggtg attatctttt ttgttgctat tttaaccagc cttgccacct
     9781 gggtagttcc ggtggggatg tttgacagtc aggaagtgca gtatcaggtt gatggtcaaa
     9841 caaaaacacg caaagtcgta gatccacact catttcgcat tctgactaac gaagcaggcg
     9901 aacctgagta tcaccgcgta cagctgttca cgacgggcga tgaacgcccg ggcctgatga
     9961 acttcccgtt tgaaggatta acctcaggat cgaaatacgg gacagctgtt ggcatcatca
    10021 tgtttatgct ggtgattggc ggcgcgtttg gcattgtgat gcgtacagga accattgata
    10081 acggtatcct ggcgcttatt cgccataccc gcgggaatga aattctcttt attcctgcgc
    10141 tgtttattct gttttcactt ggcggcgcgg tatttggtat gggagaagag gccgtcgcct
    10201 ttgccattat catcgcaccg ctaatggtcc ggctgggcta tgacagtatt accaccgtcc
    10261 tggtgaccta tattgccacg caaatcggtt ttgccagttc gtggatgaac ccgttttgtg
    10321 tggtcgttgc tcaggggatt gccggcgttc cggtgctttc tggctccggg ttgcgcatcg
    10381 tggtgtgggt tatcgccact atgattggcc tgatctttac catggtgtac gcctcacgag
    10441 tgaaaaagaa tcctcttctg tcacgcgtgc atgagtccga ccgcttcttt cgtgaaaagc
    10501 aggcggatgt tgaacaacgt ccgtttacct ttggtgactg gctggtattg attgtcctga
    10561 ccgccgtaat ggtctgggtg atttggggcg tgatcgttaa tgcctggttt attccagaaa
    10621 ttgccagcca gttcttcacc atgggtctgg tgattggcat catcggcgtt gttttccgcc
    10681 ttaacggcat gacggttaat accatggctt catcctttac cgaaggggcg cgaatgatga
    10741 tcgcccctgc cctgctggtg ggtttcgcca aagggatttt gctgctggtc ggtaatggtg
    10801 aagcgggtga tgccagcgtg ttaaatacca tcctcaacag cattgccaat gccattagcg
    10861 gtctggacaa cgcggtcgcg gcctggttta tgttgctctt ccaggcggta tttaatttct
    10921 tcgtgacgtc cggttctggt caggcggcgt taaccatgcc gttactggca ccgcttggcg
    10981 atctggtcgg tgttaaccgt caggttaccg tgctggcatt ccagtttggt gatggcttca
    11041 gtcacatcat ttacccaacc tcagcgtcgt taatggcaac gctcggtgtg tgcagggtgg
    11101 acttccgtaa ctggctgaag gtgggcgcga ccctacttgg actgctgttt attatgtcca
    11161 gcgtcgtagt gatcggcgct cagttgatgg gctaccacta aaaatgttaa gagccgcaat
    11221 tgcggctctt ttttcattct gccgtttcag tctctacggc ttcatttttg gcattgcgct
    11281 tcatccacaa cgctagcgct ttcagcgagt ctggagtgaa ctcatcgcag cgtgcggtga
    11341 tttcttccgg cgtcagccag caaacttcac tgacttcatc ttcctgtagt gcgaagggac
    11401 cgtgagagac gcagctgaac aatgcgcccc agacacggca atttttatct tcgaaataga
    11461 actgcccgtg ctcggcaaag gggacaccgg caatgcccaa ctcttcttcc gcttcgcgac
    11521 gcgcggattc cagcagttgc tcatcggcct ggactacacc gcctgcggtc gcatctaaca
    11581 tgccgggtaa aaagtctttt gtctcggtac gacgctggac cagaattttg cccatgccat
    11641 catgcacgac gatgtaagtt gcacgatgac gcagacactg tgcccgcatt tgttcccggc
    11701 tggcttgtgc aatgacttcg ttctcttcat tgacaatatc cacccattca gtacttgcca
    11761 aacgacgctg ttccattatc aggaaacctt ctttttctgg cgctcttacg gcgcattttg
    11821 agttgtgggt aaattacgga ttaatcgcga cctgcgcaat gatactttga tcattgagtg
    11881 cgattacact aagtacgtca ttatccagca tgccataact cgccggatta ccgcctttcg
    11941 gaatactcac cgagccgggg ttgaagtgga aaatctcccc ccgctgttct gccactggca
    12001 gatgggtatg accgtacacc agcacatcgt tctggtttaa agcaggcaga ttttccgggc
    12061 caaaaagatg accatgcgtc aaaaacagac gttgtttttc cagtaatacc tgttgccacg
    12121 gcgcggttat cgggaaatgc agcagcattt gatccacttc gctgtcgcag ttgccgcgca
    12181 cagcgataac cttatgtgcc acttcattaa gccgttcagc gactttggct ggcgcgtaac
    12241 cctccggtaa agcattacgc gggccgtgat tcaacacgtc gccaaggatc accagccact
    12301 gggcaccgct ttgggcaaac aactccagaa cacgttccgt cgccggtaac gacccatgaa
    12361 tgtccgatgc aaacatcagt ttcatcactc actcctcgtc gaaaaaacgt aagctatgat
    12421 actggattct gtcgttgcta tcagccagat tgcttcgccg aaagtgcaat aaaacgctgg
    12481 acagacgctc gctgccactg gaatgttgca taatccacca ggcgttctgg cacctcatcg
    12541 ccatgtagta ccaggcggtt aatcattagc gccagatcag tatcagcaat gcaccattca
    12601 ccaaataaat tcggctgacc cagtactaac aaatgttctg ccatcgcgaa cagtttctct
    12661 gcactggctt ttccctcggc cgttagtggc gcttttttcg cccccgcaaa gacaacatcc
    12721 gtcggacgct cttcgcggat gggcatcaga tcgctgcgca accaggcctg aatctgtcgt
    12781 gcacgtgcac gattttctaa atcaagcgga taaatacgtt cccaggtcgg tggcgcaaat
    12841 cgatcttcca gatactccac aatggcagaa gattcactca actcaaaatc atcgatttgt
    12901 aataacggca cacggcgtgt ctgaccgtaa ccttgccacg tcggctgcaa atgttcaccg
    12961 ctgtcgaggt cgatggtctt gatatgaaac gacaggcctt tttcctgcaa cgccacccag
    13021 gcggataaca cataagggga gaaaaagtgg gcatctgacc aaagcgtgat agcgggttta
    13081 ctcattatgt cctcggcaat aaagggggct ggctatcaaa ctatagagcc tttttgtcac
    13141 tgtcacgtga cgaatactca cgtaagtcgc cgcgctttct atacttgtgg tgttcagatg
    13201 attaaacaac cggagctgca atgatcgatc tctatttcgc cccgacaccc aatggtcaca
    13261 aaattacgct gtttctcgaa gaaacagagc ttgattatcg cttgattaag gtagacctgg
    13321 ggaaaggcgg tcagtttcgt ccggaatttt tgcgcatttc gcccaacaac aaaattccgg
    13381 caattgttga tcattctcct gccgatggcg gcgaaccgct aagcctcttt gagtctggtg
    13441 ccattttgtt gtatctggct gagaaaacag gactcttttt gagtcatgaa acgcgtgagc
    13501 gcgccgccac attacagtgg ttattctggc aggtaggcgg actggggccg atgcttgggc
    13561 aaaatcatca ttttaatcac gcagcccccc aaaccattcc ttacgctatt gaacgttatc
    13621 aggttgaaac tcagcgtctt taccatgtac tgaacaagcg gctggaaaac tcgccctggc
    13681 tgggaggcga gaactacagc attgcggata ttgcctgctg gccgtgggtt aatgcctgga
    13741 ctcgccagcg aattgaccta gcaatgtatc cggcagtcaa gaactggcat gagcggatcc
    13801 gttcgcgccc tgccaccggg caggcactgc taaaagcaca actcggtgat gagcgttcgg
    13861 atagttaaca gaaacaggtt ctcgtgtatt atttcatcct aagtaaaaca acggagaacc
    13921 tgcaatggca caacctgccg ctattattcg tataaagaac cttcgtttgc gtacgtttat
    13981 cggaattaag gaagaagaaa ttaacaaccg tcaggatatt gttatcaatg tgacgatcca
    14041 ctaccccgcc gataaagcgc gcactagcga agatatcaac gatgcgctga attatcgcac
    14101 cgtaacgaaa aacattattc agcatgtaga gaataaccgt ttctctttgc tggaaaaatt
    14161 aactcaggat gtgctcgata tcgcacgtga acatcactgg gtgacgtatg ctgaagtgga
    14221 gatcgataaa ctgcacgcgc tgcgctacgc cgattcggta tccatgacct taagctggca
    14281 gcgttaatcg ccatatcggg aggctgcatg aacatagtga tcaccggagg gacgggatta
    14341 attggtcgcc atttgattcc gcgtttgctg gagctgggcc atcaaattac ggtagtgacg
    14401 cgtaacccgc agaaagccag ttccgttctc ggccctcggg tgacactatg gcaagggctt
    14461 gccgatcaaa gcaacctcaa cggcgttgat gcggtaatca acctggccgg agaaccgatt
    14521 gctgataaac gctggactca cgagcaaaaa gagcgtctct gccaaagccg ctggaatatc
    14581 acgcaaaaac tggtcgattt gattaatgcc agcgacacgc caccgtcggt actcatttcc
    14641 ggctcggcaa cgggctatta tggtgactta ggtgaagtgg tggttaccga agaggaaccg
    14701 ccgcataacg aatttaccca taaactctgc gcccgctggg aagaaattgc ctgccgggcg
    14761 caaagtgaca aaacgcgagt gtgcctgctg cgtaccggtg tagtgctggc accggatggc
    14821 ggtattctcg gtaaaatgct gccgccgttt cgtcttggcc tgggcgggcc gattggttcc
    14881 ggtcggcagt atctggcctg gattcatatc gatgatatgg tcaacggcat tctctggctg
    14941 ctggataacg agctgcgcgg gccatttaat atggtttcgc cctacccgat acgcaatgaa
    15001 caatttgccc atgcgctcgg tcatgcgctg catcgcccgg ctattttgcg cgtccccgca
    15061 accgccattc ggctgttaat gggcgaatct tcagtactgg tattaggtgg acaacgcgcg
    15121 ctgcctaaac ggctggaaga agcgggtttt gcgtttcgct ggtacgattt agaagaggcg
    15181 ctggcggatg tcgttcgctg atgtggttta ctgcaaacat ccgccagtta actcccggtg
    15241 ttacagggtt agtggctttg cgcgataaga tcgtctggtg aaagtcgggt cagcgtcagg
    15301 atgacttcgt ggtcaaatcc tttttccagc atctcctgag caatacgcag agcttcttcg
    15361 tgtttgccct gcattgcgcc ttcttcacgt aatctgtcag caatggtcat cagtttctcc
    15421 ttttcttgtg gtgcgcgttc tgttatctca ccaataaatg cacggaaacg ctgggcatct
    15481 cccgtttgta atacataatt aaacagggct tttagctgtc tgtcattagt cttccctgta
    15541 actagcagcg aaacaatttg gtcgactaat cccaacagat cgcgctgacg aatatgtttc
    15601 tgaattaact ccaacagcgc cattttgcga tgttgcataa tctcgtcatc cggcaccacg
    15661 gtaatatcca ccaacggaaa agccgatgaa tatattttgc gggctatagc aggctcggca
    15721 aattcatcaa gccagcagag tgaataagga taaggacttc tgcaatcatg ataaaacagc
    15781 atcgggatca ccaatggaag ctctttatag cccgcatcaa gatggttttg cattgccgca
    15841 atggaataac gcatcatgcg aaaagccatt aattcttccg gcttactttg gtgctctatc
    15901 actacataaa tataacccac tccctcctgc gttttcacag accacaagag gtcggaataa
    15961 tattgccgca ggtcgtcatc aataaaactg tttggttcca gtttaagcgt cgttaaatca
    16021 cacagtttgc gcagcggcgc gggaagatga atatcaataa aatcccgcgc ggtgtctgga
    16081 tggcgtcaaa aagatttaaa taccgcatca tgcggcgtgg aagttgtcga tattgtcatg
    16141 gcgttccgtc acccctcaaa tttaagatga cgcgacaata accacgatta acacccaatg
    16201 cagcaggcag tttttatctt tggacagcga attcagaagt atttttagcg gactgcaact
    16261 tttacatttc agcggaagct gactcgcaaa ttccccatga aaatactccg gcagcgttag
    16321 ccaccggaga ggaaattatt tcagcgatcc cttaaggaac tgttgcagac gagggctttg
    16381 cgggttgcca aataactgct ccggcgcgcc ctcttcttct attttcccct gatggaggaa
    16441 aatgacgtga gtagaaacat ggcgagcaaa gcccatttcg tgagtcacta ccaccatggt
    16501 tttcccctct tctgccagtt gctgcataat acgtaacact tcgcctacca gttcaggatc
    16561 gagcgccgag gtaggttcat caaacagtaa aacttccggt tccatcgcca gcgcccgcgc
    16621 gatagaaaca cgctgttgct gaccgccgga aagatgcacc ggatatttcc cctgcgcacg
    16681 ttcgtctatc ccgactttcg ccagatactt caccgcccgc tcgcgcgctt cctgcttgct
    16741 caggcccaac acctgaatcg gcgcttccat gacgttttcc agcaccgtca tatggctcca
    16801 gagattgaag tgctggaata ccatcgtcag gcgtgtgcgc agtaagcgca gttgattttt
    16861 atcggcgact ttgagttgac cgtctttgtc gcgcaccaga ttgatcgtct ggccattgac
    16921 cacgatcgac ccttcactcg gtttttcgag gaagttaatg cagcgcagaa aggtactttt
    16981 ccccgatccc gacgatccga tgatgcttat tacatctccg gcattcgctt gcagtgagac
    17041 ccctttcagc acttcatgtt tgccgtagcg tttgtgcaaa tcgataacgt ttaatttatt
    17101 ctcggacatc gtattctcag tgcgttgaag aaggtttcac atgctgcaac cagcgttttt
    17161 ccgctctgcg aaagaggctg atcaggacat aagagatgat taaatagagc accgcggcaa
    17221 tgccgaaggc ggtaaaaggt tgatacgtgg cggcgttaat atcgcgggct attttcagca
    17281 gatccggcac agtggccgta aacgccagcg cagtagagtg cagcatcagg atcacttcgt
    17341 tgctgtatgc cggtaacgca atacgcagcg cagaaggcaa aataatgcaa cgatacattt
    17401 taaaagtcga gaagccatag gctctggcgg cttcaatttc cccatgcgga accgaacgga
    17461 ttgccccagc aaaaatctcg gtagtgtaag cgcaggtgtt aagcgtcagc gccagcacgg
    17521 tacagttcag gccactgcgg aagaaagcgt taaggaattc ggttccctta acaatctcaa
    17581 gcgtgtacat gccggaatag aacaccagca actgaacata cagcggcgta ccgcgaaaaa
    17641 tataggtaaa taaccagatt ggaaactgga tgtatttatt actggagaca cgaccaatcg
    17701 ccagaaacag cgccaggact ccgcctatca ctaccgacaa aataagcagc cacagagtga
    17761 tcgccacacc agtaaagcga taaccgtcgg tccacagcag cggtttccag tattcatgta
    17821 agatttcgat cacaggtcag ccctcttcac acccacggag tagcggcgct caaggaacag
    17881 cagcacacca ttggaaacag tggtgaaaac caggtaaatc acgccacaga cgatggcgaa
    17941 atagaacggt tcccaggtac ttttgcctgc cagttgggtg gctttgacca catcttccag
    18001 gccgagtaac gaaaccagtg cggtagattt gaggatcacc tgccagttgt tgccaatgcc
    18061 tggcagggcg taacgcatca tcgccggaaa catgatccgc cgaaacactt gcccacgagt
    18121 aaaaccgaac gccgtcgccg cctctatatg tcctttcggc actgccataa aagcaccacg
    18181 gaacgtttcg gtaaaataag caccgtagat aaaaccgaga gtgataatac cagcgaccat
    18241 cggatcgata tcaatctgcc cgacgcccat cgcctccgtc accgtgttta gcgcaatctg
    18301 caaaccgtag aaaatcagca gcatcaacac taagtccggc acgccacgaa tcagcgtggt
    18361 gtacccttcg aaaatcagcc ccgaaagccg attttgcgag agcttaccgc cagcgccaat
    18421 taaaccgatg attacagcga gcactacaga gctgatagcc agctccagcg tgacgagcgc
    18481 accctgtaaa ataacacctg aaaacccata caacatgctg cctgtcctgt cgtgtgtggt
    18541 gaattaccgc ctcttgtctc cctccggtta cccgaaggga gcgggctttc tttcaccgat
    18601 ggcgattagc caccataaac atcaaaatcg aagtactttt tcgctaattt ctcgtaagta
    18661 ccgtcagcgc gcatttcggc aaaggctttg ttcagtgctt cgcgcagttc gttatcttct
    18721 ttacgcaggc ccatgccggt ccctacgcca aacagttttt catctttaac agacgggcca
    18781 ccgaatttgt aatctttacc gacaggttgt ttgaggaaac cttcgctggc ggcgacctca
    18841 tcctggaacg cggcatcaat acgtccggca gtcaggtcag aataaatgtt gtcctgcccc
    18901 tgatacgaga cgatttcaat gccttttggt gcccaatgtt cattaccgaa cgtctcctgg
    18961 gtggtgccct gcaatacgcc tacccgtttg cctttcaacg actcgactgt cggctgaatg
    19021 tcagaatttt tcgccaccac caaacgagaa tcggcagcgt acagtttgtc ggtgaaggct
    19081 atttcttgct gacgtttttc cgtaatggaa agcgatgaca tgatggcgtc aatcttcttc
    19141 gcttttaagg acgggattaa cgcatccagc ggattttcga caaacgtaca ttgcgtattg
    19201 atgcgtttgc ataattcctt tgccagatcg atatcgaagc caaccagttc gccttgtgaa
    19261 ttttttgatt caaatggcgc ataggtcggg tcggtaccga tgcggatgtt ttgcggaatc
    19321 gcagcaaatg ccgcagttgc gctggagaag gccagaacca gagagagcga tagcaccagt
    19381 tttttcataa tgtcctcaac tgacagtctt tttataggga atatttacag gtttgttaca
    19441 cttatcgtgc cataaaattg accatcaagg caaaatatcc cgccccgtta gacgtattat
    19501 ttttcattaa aaatgcttaa gtatgcattt caatgcacca cgatagtgca acaaacctgg
    19561 cgtgcaccat gatgatcatt ccatcaggta cagcttccca gcgacgtatc agtcaccgta
    19621 gacattaaag tcgaaatact ttttcgccat cttgtcgtag gtgccgtcct gacgcagctc
    19681 gccaagcgcc ttattgaagg cagccgtcag ttcagcatca tctttacgta gccctacacc
    19741 ggtgccatca ccgaagtatt ttttgtcttt tactgatgag ccagcaaagg cgaaatcttt
    19801 accagcaggt tgcttgagga atccttcgct ggcagcaact tcatcttgta acgcagcatc
    19861 cagacgtcct gcagccagat cggaatagac caaatcctgg ttggcatagg ccaccacatc
    19921 cacgccttta ctacgccagg tctcgttagc gtaagcttcc tgggttgatc cctgcagcac
    19981 accaacatgt ttacctttca gtgaatccag cgttggctga atcggtgaac ctttggccgc
    20041 aatcaaacga gaatctgcgg cgtacagctt gtcggagaag gcaatctcct gctgacgttt
    20101 atcggtaatg gaaagcgacg aaataatagc gtcgattttt ttcgctttca gtgaggggat
    20161 cagcgcgtca aagtcactgg caacccaggt acatttcacc tgcatccgtt tgcacatctc
    20221 gttaccgaga tcgatatcaa agccaacaaa atcaccttta gcatctttcg atgagaacgg
    20281 tgcgtaggtg gtatcggttc cgatacgtac cgtctccggt agcgccgcat agctggaagc
    20341 cgctgtggag agaccgacta acaaagacag agcgagaatc gacttcttca tacataaccc
    20401 tcaagtgaaa atggcttttt tatgttgtgt attgtgttgt gtgtttgcag gctctttcat
    20461 gcaggtctta tgccatcttg acgacaacag taacattcaa cgttaaatat gttaataaga
    20521 cgttgcatta ttgtcctgaa gttgaagata gcaggtatag cggttgaatc gcagcgtttc
    20581 gattgaatgg cagaaacaaa atgtcgagga tttgatcgcg gttgcaaaat tgccctgaaa
    20641 cagggcaaca gcggagttat gcgccctgcc agcgggcaaa gagatcttca ggaagggtta
    20701 tcgcaaactg gtcaagaaca cgattaaccg tctgatttat cacatcatca agggattgcg
    20761 ggcgatgata aaacgccgga acgggaggca taatcaccgc accgatttct gccgcctgag
    20821 tcattaaacg cagatggcct aagtgcaatg gtgtttcacg cacgcagagc accaacgggc
    20881 gacgctcttt cagcaccaca tctgccgcac gggtcagtaa gccatcagta tagctatgga
    20941 caatgacgga aagggttttg attgaacagg gtaaaatcac catccccagc gtctggaaag
    21001 aaccggaaga gatgctggcg gcaatatcgc gcgcatcgtg cgtgacatcg gctaatgcct
    21061 gcacttcgcg cagagaaaaa tccgtttcga gggataaggt ctggcgcgct gcctggctca
    21121 tcaccagatg cgtttcgata tctgtgacat cgcgcagaac ctgtaataag cgcacgccat
    21181 aaatcgcgcc gctggcaccg ctgatgccta caatgagtcg tttcataaat agttgccctg
    21241 tcagacttgc aggcagactt tgcaggattt cggcgggagt tgcaagtcag ggtgccagac
    21301 cggcaccctc agcgaaggca tcatccttcg ttatgcattt cgagattttc cacttcgttc
    21361 tgacgttgca ctgctttggc gtcatcatta cgtaacgtat cgaggaaatc gaggtagccc
    21421 tgatcaacgt ctttggtgac gtagacgccg ttgaataccg agcattcaaa ctgctgaata
    21481 tccgggtttt ccgcgcggac ggcttcgatc agatcgttca gatcctggaa aatcaacccg
    21541 tcagcaccga tgatctggcg aatttcatcc acttcacgac cgtgagcgat tagctccgtg
    21601 gcgctcggca tatcaatacc ataaacgttc gggaagcgaa tttccggtgc cgcagaagcg
    21661 aggtacactt tcttcgctcc ggcttcgcgt gccatctcga taatctgctc agaagtggtg
    21721 ccacggacga tggagtcgtc gaccagcagg acgtttttat cgcggaactc ggcgcggttg
    21781 gcattcagtt tacggcgcac ggacttacga cgcagctgct ggcccggcat gataaaggtg
    21841 cggccaacat agcggttttt aacgaagccc tggcggtacg gtttgcccag aatacgagca
    21901 atttccagcg cgatatcaca cgaggtttct gggatcggga tcaccacgtc gatatccaga
    21961 tcttcccatt cgcgggcaat tttctcgccc agtttcgtgc ccatattcac acgcgcgctg
    22021 taaacggaaa ttttgtcgat gaacgagtcc gggcgagcaa agtatacata ctcaaacagg
    22081 cacggattgc tgaccggatt gtcagcacat tgacgggtaa acaactgccc ttcttcagtg
    22141 atgtaaatcg cttcgcccgg cgcgacgtca cgcaggaaat caaagcccag cgtatcgagc
    22201 gctacgcttt cggaagcgac catatattct gtacggttct cgtcaatatc acgttttccc
    22261 agtaccagcg gacgaatccc gtttggatcg cggaaagcaa ccataccgtg gccgataatc
    22321 atcgccacac aggcatacgc gccgcggatt aagcggtttg tggcagcaat ggcagcgaaa
    22381 atattgtcgg cttccagcgg gtagtggcgg aaattgtcca gctcgctggc gaagatatta
    22441 agcagaattt ccgagtcgga agtggtgttg atgtggcggc gtttttcttc aaacagtttt
    22501 ttacgcaact cgtgagcgtt ggtcagattg ccgttgtggg caagcgtaat gccatacggg
    22561 gagttaacgt aaaacggctg cgcttcagag gcgctggagc tgccagccgt ggggtaacgc
    22621 acatgaccaa tgcccatatt gccctgcaaa cgctgcatat ggcgagcttc aaatacatcg
    22681 ctcaccagcc cgttcgcttt acgcaaacgg aagcagttat tggcatctat ggtgatgatg
    22741 ccggcggcat cctgaccgcg atgctgaagc accgttaagg catcataaat cgactggtta
    22801 accggcataa caccggcgat accgacaata ccgcacatac gtctttttcc tcgttaagcc
    22861 acatctcaga gcacttacgc tctgggcaag aaacttgacg agctttgcag ataatcaaaa
    22921 aaccatctga tgataaaact gaattgcggg atcagctgtg atttgctcca gtcttcgctt
    22981 ttcgacaccc cggtaaagga gtcgagaaag aagagaatgg cagcaacaat caacacaccg
    23041 cgcaacgcac cgaaacagac gcccagcacc cgatcggtgc ctgacaaccc cgttttctcc
    23101 accaactggc ctatcacgaa gttcacgata gcaccaacga tcagggtagc gataaacagt
    23161 accgcgatgg caatcccatt tcgaaccagt tcgtcttcaa agcccgtaaa ccagactgac
    23221 aggtaagtgt agtaatgact ggcaacaaag aaagcacaac cccatgtcac cagcgataac
    23281 gcttcacgaa caaagccgcg gatcaggcta accagagagg aaaaagcaat caccgcgatt
    23341 atggcgtaat caatccagac catatgtgtc ccacgatttt acgccctgtc atcctgttcg
    23401 gggcgcattc taacagaaaa agaaaacgtt tgcgtaggga tttccttccc gcgcatcaat
    23461 aaaaatggcg ctgaaaaaat attcaacgcc atcgactttt tatgcctttg cggcatcggg
    23521 caatgcgtgt cggatgcggc gtaaacgcct tatccgacct acggttctac ccctgcgtag
    23581 gcctgataag acgcgccagc gtcgcatcag gcaagaccgt attaattcgg cgtatagccc
    23641 attaccacgc cacttaagcc agaaagttgc ttcaactcac ccagcgaacc tttcagctta
    23701 tctttcgagg catccggccc aaccagaata cgggtaattt taccctgcac tggcgtggat
    23761 ggcgacgtat aaacccgata accggcaccg cgcagcttac cgacaatctc attcacttta
    23821 tcggcatttt tcagcgcacc cagttgcaca acataggctt tacccgtcgg tgcagctttt
    23881 tcttccacga ccggcttcgg ttctggcttc ggcgcaggtg gtgcttcaac ctttggttta
    23941 ggcggctcca ccggtttcgg cttcggtggg gcgaccggtg caggttccgg ttcaaactcg
    24001 gtgttattgg ctgcaatagt ggccggatcg agcgacggtg ctgcggcatc acctgcccgc
    24061 acctcttccg ctgcgccttc cggcggctgc gtcggtaacg cctgggtggc ggctggcatc
    24121 atatcaggct catcacgatc gcccgctttc ggcaccagcg ggatagccgc gaactcatcc
    24181 tgataatgtt ttttctgccc gtccagcagc cctggaagta caatcacccc cagcgccacc
    24241 agcacgatcg tgcccactaa ccgattctga aacttacttg ccaccgcttc tcctcgcgtc
    24301 aatcacttcc atgacatgtg cgaccgtgtg gaaagaacca cacaccagca cggtgtcttc
    24361 cgctttagcg tccgccattg cggcatccca tgcctgcgca acgctatcaa atgatttgcc
    24421 gttacccaaa tgctcaagca gttgttctgc cgtggcaccg cgcggccctt ccagtggcgc
    24481 acaataccag tcatcaacca cgcttttcaa ccaggccaga gttccggcaa tatctttatc
    24541 atgtagcata ccgataaccg ccagcacgcg cccgtttttc ggtagcgctt tcatacgccc
    24601 ggtgagatat tccgccgcat gtggattatg cgcgacatca aaaataacgc gtggcgactc
    24661 gctcacaatc tggaaacgtc ccggcaaaat tgcgctggca atcccgtcgc gaatggcatt
    24721 ttcactgact tccagcccgc tggcacgcag tgccgccagc gctgttgcgg cattcggttg
    24781 cgggacaagc ggcaacggca gattttccag cgtgccgtga gcatcgctaa acgcccagtc
    24841 atgatcggtg acggaatagt tccactcaac gccccgacgt tgtaacagtg cacctttttc
    24901 ctgcgccaca tcagcaatgg tagaaggcat ttccggctca ccgacaattg ccggtttttc
    24961 gctgcggaag atgcctgctt tctcgcgacc aatactttcg cgatctggac ccagccagtc
    25021 ggtatgatcc agcgcaatac tggttactac cgcgacatcg gcgtcgacaa tattggttgc
    25081 gtccagacga ccgcccagcc ctacttccag aatcaccacg tcaagttgtg cctgcttgaa
    25141 cagccacaac gccgacagcg taccgtactc gaaataggtc agggaaatat caccgcgtgc
    25201 cgattcaatc tccgcaaaag aggcggtgtg ggccgattcc ggcaattcct ggccctgcac
    25261 acgtacgcgc tcggtataac gcaccagatg aggcgaactg tagacgccca ctttgtaccc
    25321 tgccgccatc agaatcgact ccagcgtacg gcaggtggtg cctttgccat tcgtacccgc
    25381 aacggtaaac acaaatggcg ctggtttcag gacgccaaga cgcgccgcga ccaggctcac
    25441 gcgctcaagg ccgagatcga tagttttact gtgcaggttt tccagataag aaagccacga
    25501 agccagaggc gacgcggctt gaggagtgcg tttgataatc atggtatccg ctgattcgtt
    25561 acggtgagaa tagcaaaagg gcagagccag tggccctgcc cttatcagtt atcaggcctc
    25621 aggttcctga tccggtaccg ggggtaccac tacgccttca cgcggcgctt caggattcgg
    25681 cgctggcaga ttcatcaact tcgccagaat gctcgccagt ttcaggcgca tttccggacg
    25741 acggacgatc atgtcgatcg cgcctttctc gatcaggaat tcactgcgct ggaatccagg
    25801 cggcagtttt tcgcgaacgg tctgttcgat aacacgcgga ccggcaaagc cgattaacgc
    25861 tttcggttca gcgatgttga gatcgcccag catggcgaaa cttgcagaaa caccgcccat
    25921 cgtcgggtcg gtcagcacgg agatgtacgg caagccgcgc tcctgcattt ttgccagtgc
    25981 cgcagaggtt ttcgccatct gcatcagcga catcagtgct tcctgcatac gtgcgccacc
    26041 agaggcggag aagcagatca gcgggcagtt atcttccagc gcctgctcaa cggcacgcac
    26101 gaaacgtgca cccacaacag accccattga accgcccata aaggcgaact cgaatgccgc
    26161 agcgacaacc ggcattccat acagagtgcc tttcatcacc accagcgcat ctttttcgcc
    26221 ggtttctttc tgcgcagatg ccagacggtc tttatacttc ttggagtcac gaaacttcag
    26281 cacatctttc ggctcaagct cgctacccag ctccacaagg cttccttcat ctaacaggct
    26341 atgcaggcga ttacgcgctg tcatacgcat gtgatggtca cacttcggac agacctcaag
    26401 attacgttcc agctcagcgc ggtataaaac ctgaccgcag ctatcacact tagtccacac
    26461 cccttcagga atgctcgcct tgcgggtggg agtaatgttg cttttaattc gttcaatcca
    26521 gctcattagg gacctttctg tctgaacctg gttcgatgcc agttttatct ttggggacgc
    26581 ataatgccat ttttgccccc aacagaccat gaatgttgca cattaaaaca taacagcccg
    26641 aaactttgga taaaaaagtg gtcgaaccgc ggagttactt tttattttgc ggcgcgtgcc
    26701 gcagcgcgtt tgtgacggat tatttcgatg acgcccggca aaatagaaac cacaataatc
    26761 ccgacgatca gcagcttaag gttatcctga accatcggga ttgtaccgaa gaaatagcct
    26821 gcgtaggtaa aaagcagtac ccacaacagt gcgccgatca cgttataagc ggcgaaatga
    26881 cggtacgaca tgtggcccat tcccgcaaca aacggggcga acgttctgac gatcggcaca
    26941 aaacgggcga gaataatcgt tttgccgcca tgtttctcat aaaactgatg ggttttgtcg
    27001 agataactgc gacggaaaat tttcgaattg ggattactga acaacttttc accgaacaac
    27061 cgcccgatag tgtaattgac cgcgtcaccc acaatcgcgg cgatcagcat cagcaccacc
    27121 atcatatgga cgttgagatc gttggtttcc agtgatgcca acgctccggc aacaaacagc
    27181 aatgaatcac ccggtaaaaa cggcgttacc accagaccgg tttcacagaa caaaattaag
    27241 aacaaaatgg cataaaccca gacgccgtac tccgcgacca gctctgccag atgcacgtca
    27301 atgtgcagga taaaatcgat gaggaaataa atcaggtcca tatttgccta tgccttgtac
    27361 tcgtcatctt tcaggctgta actacgttgg ctacagcctg aaatgttccg agcattattc
    27421 tcgttagtcc gccagaaata gcgggcccat tggcggtttt ggaagatcat accggtcagg
    27481 gtaatccacc gcgaccagat acagcccttc cgcttttgcc gttgctgccg ccagcgttct
    27541 gtcctttgcc gccagcaact ctgctatcca gctctccggc tggttgtggg caccgacttc
    27601 catcaggctg ccgacaatat tcctgaccat atgatgtaca aaagcattcg ctttaatatc
    27661 taccaccaca taaggaccgt gacgcgtgac attaatatgc ataacgttgc gccacggggt
    27721 tcgggactgg cactgcaccg cacggaacga ggtgaaatca ttctcgccca gcaagcattg
    27781 cgcagcccga tgcatccgtt cagcgtccag cggttcgtaa aaatgggtta cccctttact
    27841 cagtaccgcc gggcgcagcc gatgattgta gatgatgtag cgataacggc gagccgtggc
    27901 gctaaatcgg gcatgaaaat catcaggtac agctttaacc caacgcacag cgatgtcacc
    27961 aggtaaattc gcatttacgc ccaaggtcca cgccgcgtct ttgcgcagcg cggttgtttc
    28021 gaaatgcaca acctgcccgg taccgtgtac ccctgcgtca gtacgcccgg cgcagaagac
    28081 ggtgatgggt tcgttcgcca cctgggagag cgccttttcc agcttctcct gcacactgcg
    28141 aacttcgttc tgccgttgcc agccgtaata cttactgccg tcgtactcaa tgcccagcgc
    28201 aattttataa actggcggtt gttgctggtc ggacattagt acagatactc ctgcaccagt
    28261 ttctcggcga tttttactgc catcagcgcg ccgccaaagc gaacgttatc ggccaccgac
    28321 cagaactgga cttgctccgg cataccgtag tcattacgca cgcagccaac agaaagatgc
    28381 ggcgtacccg aagcatcacc tacctgagtt gggaattcgt tctcttcaga gagcacaata
    28441 tcttcgcctt gaacaaacgc atcacgcgct tcttctgctg ccagtggacg cagagcttca
    28501 aagttgacca tctgggcatg accgtagaat accggtgcct ggacgacgct agccgaaatc
    28561 atcagccctt cgtcctgcag gattttgcgt acttcgtcaa cgatacgacg ttcttcacgc
    28621 acgctacctt cgctatccgg cagtaacggc agcatgttga acgccagctg acgcccgaag
    28681 aaatcttctt cgtcaatcgg aatgccgttg agcaatttcg cactctgccc cgctaacgca
    28741 tcgaccgctt ttttgccctg ggcggaggct gaaatcaggc tggtaacgct gatacgtgat
    28801 aaaccgccct gatcgattaa cggtttcagt gccgccagca gctggctggt cagactgtct
    28861 ggtacggcga tgacattccg gttccggtaa tctgtcagta caaacgggtt tacttccggc
    28921 accaccagcg gtacgtcggg ttcgagagca aacaatccac tgctgtcgat caccaggcaa
    28981 cctgagttgg tcgcttcttc aacccaggca gcggtagctt ctttgcctgc gacaaaaaat
    29041 gccagctgcg cctgcgtcca gtcgaattca gcggcatcct gcacggtgat tgtcttacca
    29101 ccaaagcgca gttgttcgcc tgcgctttcg ttacgtgcca gtgcataaat ttccccaacc
    29161 gggaactgac gttcagccag cgtttcaagc agggcttcgc ccacagcgcc agttgcgccc
    29221 aggacggcaa tgttccagcc ttcagacatg gtggtttact ccagaaatag caaagctccc
    29281 tgccaatgtt acagcagaga gcatgaagaa gagattaacg tgccggatga tgaacggcgt
    29341 taaaacccag tttacacagc aatgatgccg cactggcgtc atcacaaatt acatacagag
    29401 atgaccattc acggcgctca agatagtttt tgcgcagttt atcgaactca cccggtatcc
    29461 cggcgacttt acgcagcggt gcgtcatcgc ggcgcacatc atacaccaaa tgcaccagcc
    29521 ttttcagcgt cggttgatcg agcgggccat gcagcgtaat gcgaccaaac tctggcgcgg
    29581 gcagtaatgt atccagcgca acgtgctgtt catgcccaat aaacttgcta taggcttcaa
    29641 acacttgcgt agtaccgcgt gctttacctt ccagggtata acctgcgata tgcggcgtgc
    29701 cgatatccac ttttgtcagc aactccacgt taagttccgg ttcgccttcc cagacatcca
    29761 gcactacgct taacttctgg ccttcattca ggcaggtcag caacgcagta ttatcgacga
    29821 ctgcgccacg gcaggcgtta atcagaatcg ctcccggctt caggctacgg atcagttttt
    29881 catccgccag atgcagcgtt ttgtacggcc catctttgaa gagtggcgta tggaaagtca
    29941 gaatatccgc atgctgaact aattcatcca gcgagcggaa atcaccctca tccccacggt
    30001 cagcgcgagg cggatcgcaa agcaatgttt taatccctaa cgcttccagt cgcgcctgca
    30061 atcgacgtcc aacgttacca acgcccacga tccccaccgt acggtcgtgc agtgaaaatc
    30121 catcgcgttc ggcaagcatc agtagtgagg agaaaacata ttccaccacc gcaatcgcat
    30181 tacagccagg tgcagcggaa aaaccaattc ccgcctgctt caaccatgct tcatcgacat
    30241 ggtcggtccc cgcagtggca gtgccaacaa atttaatggg ttttcctgcc agcaaagatt
    30301 cattcacttt cgtgaccgaa cgcaccatca gcgcatccgc gtctgccagt tgagcgacgg
    30361 ggattggacg cccgggaacc gcggtcacct cacccaaacg gctaaataag tcgcgggcat
    30421 aaggcatatt ttcatcaaca aggattttca cgtttgtgtt acctgtatga gacgagagtt
    30481 aaccggacaa gtgtgccata atctcgcggc caggcatact tgcgaagatt tcaggtataa
    30541 ggatacgtaa tgatacaacc tatttccggc cctcctcctg ggcaaccacc aggtcaggga
    30601 gataacctgc cgtctggcgc gggcaatcag cctttatcca gtcagcaacg tacttcgctg
    30661 gaaagcttaa tgacgaaagt gacctcactg acgcaacagc aaagagcaga actgtgggcg
    30721 ggtatcaggc acgatattgg tctgtcggga gattcaccgc tgctttcgcg tcacttccct
    30781 gccgctgagc ataatctggc gcaacgtctg ctggccgcgc aaaaaagcca ttctgcccgc
    30841 cagcttttag cgcaattagg ggagtattta cgtctgggga ataatcgtca ggcggtcacg
    30901 gattatatcc gtcataactt tggtcagacg ccgctgaatc agctctcacc ggagcaatta
    30961 aaaaccattc tcaccctgtt gcaggaaggg aagatggtta ttccgcaacc acaacagcgc
    31021 gaggcgaccg accgtccttt attaccggcg gagcacaatg cgctaaaaca gctggtgacc
    31081 aaacttgcgg cggcaacggg ggaacccagc aaacagatct ggcaatcgat gctggaactt
    31141 tccggggtga aagatggcga gttaattcca gcgaaactgt ttaaccatct ggtgacctgg
    31201 ctacaggcgc gtcagacgct aagccagcaa aatacgccga cgctggaatc actacagatg
    31261 acgctaaaac aacctttaga tgccagtgaa ctggcggcgt tatcggcata tatccagcaa
    31321 aaatatggtc tttctgcgca atcatcgctt tcttctgccc aggccgagga tattcttaat
    31381 cagctttatc aacggcgggt taaagggatt gatccgcgtg ttatgcaacc gctgcttaat
    31441 ccttttccac cgatgatgga cacgttgcaa aatatggcaa cgcgtcccgc gctgtggata
    31501 ctgttagtcg cgattatcct gatgctggtc tggctggttc gttaaccccg acgaaatgac
    31561 agtatcgtga caataatacc cagcaccgca gagatcgccc cggcaagaaa taccgaagag
    31621 taaccaaacg tggtcgccag cattcccgcc agcggcccgg agacgccgag ggcgatatcc
    31681 tgaaacgcgg cgtaaccgcc cagtgcggtg ccgcgaactt gtgaggggac gcgtttaacc
    31741 acctccacgc ccagcgcagg aaagataagc gaacatccgg ctccggttaa cgccgcgccc
    31801 gctaatgcga cccatgcacc tggggcttgc cagagcagca acaagcccac cgtttctaca
    31861 agcagagaga caatcgccac tttcacgccg ccaaaacggt ccggcatcca gccaaacatg
    31921 acgcgcatca cgacaaatgc gccgccaaac gcggtaagag taaagcccgc catcgcccat
    31981 cctttgctgg caaagtagag cgaaacgaaa gtcccgataa cagcaaaacc aacgccttgt
    32041 agtgccagac ctaaccctgg tttccagata agcccgacaa cgctccacag cgatggacgt
    32101 tctcccgcca gggccggtac tttgcgcact gtgccgttac aggcccacgc cagtacgggt
    32161 aataccattg tggtgatcgc cagtgcggca aaaccgtaat ggctatgaat caacaggcca
    32221 agcggagcac caacagcgag ggcaccgtaa atcgccattc cgttccatga catcactttg
    32281 ccagagtgtt ttggccctac gatgcctagt ccccaggtca gagcgcctgt cagtaactgg
    32341 ctttcaccaa agccaagaat caaacgcccg acgaccaaca gagcaaattt gaacggtgcg
    32401 gagacaggca aaatcgccgc cagcagcaac gcgccgccag ccagaccaca agctaacatt
    32461 ccctgaagcg ccgaacgttt tgcaccatat tgatcggcca gtcgcccggc gtaaccacgc
    32521 gtcagcaccg tagccagaaa ctgaatcccg acggcaatgc cgaccatggt attgccatag
    32581 cccagttcat gatgaacaaa cagcgggata accggcaacg gcaaccctac ggtcatgtag
    32641 gtgagaaaaa ccgcaaaagc gatgcggaag agcgaaaaat tggcagaaga tcgtgtttcg
    32701 gtttggctta cagcagtcat gcattactcc agaatgcagc gcaaggcgag gagtatcccc
    32761 gtctcatctc tctggtttca gggttacggt gcgttggcag gatttaacgc gtacgtcttt
    32821 tcagaaggaa atcgacaaag cgggaagttt gcctggaact ggcggcgatt gtcaatgatg
    32881 tgaaaaaggg aaccatcagg ttcccttttg cgttagtgcc ggaggccgca ttggcgcgta
    32941 acgtcggatg cgacgctggc gcgtctactc cgacctactg cgaattaatc tttcagcttg
    33001 cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat aacggtggtc
    33061 agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc gtccagctct
    33121 tcaatgttga tgctcggggc gataaagccg tgttccagca tcagcagaga gtagatagct
    33181 tcctgtacgc cagcagcgcc cagagagtga ccggtcatgg ctttggttgc agaaatcgcc
    33241 gggctcttat cgccgaacac ttcacggata gctgccagct ctttcacgtc gccaaccgga
    33301 gtcgaagtac cgtgggagtt caggtaatcg attggggtat caacgccatg catcgccatc
    33361 ttcatgcagc gtactgcgcc ttcgccagac ggagcaacca tgtctgcacc atcagaggtt
    33421 gcgccgtagc caacgatttc agcatagatg tgagcaccac gcgccagcgc gtgttccagc
    33481 tcttcaacca ctaccatacc gccgccgcca gcgataacga aaccgtcacg gtgagcgtcg
    33541 taagtacggg aggctttttc cggggtgtcg ttgtatttag tagacagcgc acccattgcg
    33601 tcgaattcgc aagccatttc ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc
    33661 tgtttgccca gttggatctg ctctactgcg ttaccgatac agtgtgcgga agtcgcacac
    33721 gcggagctga tggagtagtt aacgccatga attttaaacg gggtggcgag gcaggcagaa
    33781 acgccggatg ccatcgcttt ggtgaccaca tacgggccaa ccgctttcag gccgcgcggg
    33841 ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg agccgccgcc ggaacctgca
    33901 atcaggccaa cgcgcgggtt attctggtaa gcttccggag agaggcccgc atcagcgatt
    33961 gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg cacaactttg
    34021 cggtcaatga ggccagtggt atccagtttt acgttgcccc agacgtggct acgcatgccg
    34081 gaatccttca gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag agatgccagg
    34141 acttcctgct ggttattacc gatgctggaa acaatgccca ggccagtaat cactgcacgt
    34201 ttcattcaat acctctgtaa gtcgcacata gagtaagttt cgaatgcaca atagcgtaca
    34261 cttgtacgcc gaacaagtcc gatcagccat ttaatagaga aatttgcgca gccttacaca
    34321 catcgctaag atcgagccac cgcctgtaag acgagtaact tacgtgaaac actactccat
    34381 acaacctgcc aacctcgaat ttaatgctga gggtacacct gtttcccgag attttgacga
    34441 tgtctatttt tccaacgata acgggctgga agagacgcgt tacgtttttc tgggaggcaa
    34501 ccaattagag gtacgctttc ctgagcatcc acatcctctg tttgtggtag cagagagcgg
    34561 cttcggcacc ggattaaact tcctgacgct ttggcaggca tttgatcagt ttcgcgaagc
    34621 acatccgcaa gcgcgattac aacgcttaca tttcattagt tttgagaaat ttcccctcac
    34681 ccgtgcggat ttagccttag cgcatcaaca ctggccggaa ctggctccgt gggcagaaca
    34741 acttcaggcg cagtggccaa tgcccttgcc cggttgccat cgtttattgc tcgatgaagg
    34801 ccgcgtgacg ctggatttat ggtttggcga tattaacgaa ctgaccagcc aactggacga
    34861 ttcgctaaat caaaaagtag atgcctggtt tctggacggc tttgcgccag ctaaaaaccc
    34921 ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc caggcggcac
    34981 gctggcgaca tttacgtctg ccggttttgt ccgccgcggt ttgcaggaag ccggattcac
    35041 gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg ctttgcgggg tgatggaaca
    35101 gacattaccg ctcccctgct ccgcgccgtg gtttaaccgc acgggcagca gcaaacggga
    35161 agcggcgatt atcggcggtg gtattgccag cgcgttgttg tcgctggcgc tattacggcg
    35221 cggctggcag gtaacgcttt attgcgcgga tgaggccccc gcactgggtg cttccggcaa
    35281 tcgccagggg gcgctgtatc cgttattaag caaacacgat gaggcgctaa accgcttttt
    35341 ctctaatgcg tttacttttg ctcgtcggtt ttacgaccaa ttacccgtta aatttgatca
    35401 tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa agccagcata aaatcgcaca
    35461 gatgttgtca atggatttac ccgcagaact ggctgtagcc gttgaggcaa atgcggttga
    35521 acaaattacg ggcgttgcga caaattgcag cggcattact tatccgcaag gtggttggct
    35581 gtgcccagca gaactgaccc gtaatgtgct ggaactggcg caacagcagg gtttgcagat
    35641 ttattatcaa tatcagttac agaatttatc ccgtaaggat gactgttggt tgttgaattt
    35701 tgcaggagat cagcaagcaa cacacagcgt agtggtactg gcgaacgggc atcaaatcag
    35761 ccgattcagc caaacgtcga ctctcccggt gtattcggtt gccgggcagg tcagccatat
    35821 tccgacaacg ccggaattgg cagagctgaa gcaggtgctg tgctatgacg gttatctcac
    35881 gccacaaaat ccggcgaatc aacatcattg tattggtgcc agttatcatc gcggcagcga
    35941 agatacggcg tacagtgagg acgatcagca gcagaatcgc cagcggttga ttgattgttt
    36001 cccgcaggca cagtgggcaa aagaggttga tgtcagtgat aaagaggcgc gctgcggtgt
    36061 gcgttgtgcc acccgcgatc atctgccaat ggtaggcaat gttcccgatt atgaggcaac
    36121 actcgtggaa tatgcgtcgt tggcggagca gaaagatgag gcggtaagcg cgccggtttt
    36181 tgacgatctc tttatgtttg cggctttagg ttctcgcggt ttgtgttctg ccccgctgtg
    36241 tgccgagatt ctggcggcgc agatgagcga cgaaccgatt ccgatggatg ccagtacgct
    36301 ggcggcgtta aacccgaatc ggttatgggt gcggaaattg ttgaagggta aagcggttaa
    36361 ggcggggtaa tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct tatccgacct
    36421 acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg catcaggcat ggtgctccag
    36481 acgccgcaac ttactgctgt gacgcctgct gaaacaggtt ttcccacata tcggtcacca
    36541 gcgcctggtc acgcggcgac aattcaccgg caccaatggc tttttccaga ctctggctaa
    36601 cggtcgtatg caccgcctga gcggagtggt catcaccact ttccagttct gcgatggcta
    36661 atgtcaggtg gccacgcaaa tacccactgg caaacaactc atcatcactg gcatggtcaa
    36721 ccataccgtc gattaatgcc agaatgcgtg attcaaactc cgcgatcatc ttctttcctc
    36781 attgtaaaac gtggcgctgg cttcagttga gcgcttccgg ccacgggaac tgttccgccg
    36841 taagttccgg cgtgtgataa taattttgta gtgctttaat gaagcgtgcc ggacgttcgg
    36901 gaatgccgtt tgccagataa tccattacct gcgcatgtac ccgccgttga aacaccacgc
    36961 ggtccggttc gaaatcccct tccagattgt cgcagctaac attaaacgga tatcccgccg
    37021 ccacgcagaa caaccagtcc agcgcctgcg gcttcacttc aacatcttca aactgacttt
    37081 gcgtctgggc atcgcgtccg tccgggcaat accagtagcc gaaatcaacc agctcacggc
    37141 gcgctttccc ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca taaaagccat
    37201 gagcaaagac gattcggtta tacggtactt ccgcatcagc aggaagatag atcggttcgt
    37261 cgtcgccttt aatcagacgg gtattaaaat catcggcaaa gcagctatta aaaatttcaa
    37321 tcaactgctc atagtggtgt gtactgttca ttagttcatc cccaaccagt ggaggatctc
    37381 ctgtccgtgg ctatcataaa gtaatttggc actcatcacc gccgagacga taacgatcat
    37441 cgggcggatc agtttttgtc ctttgctcaa caccagtctt gaacccatgc gcgcgccgag
    37501 gaactgcccc accagcatga caaagcccgt cgcccaaatc actttgccac cgagaataaa
    37561 cagcagtaaa ccgccgatat ttgacgttgc gttgagtaat ttggcgtgag ccgtggcttt
    37621 ggcgaggttg aatccgcaca gcgtaacgaa ggccagcgcg taaaacgatc cggcagctgg
    37681 cccaaagaat ccgtcgtaaa aaccgacgca gccacctgca attaacgcga agggtaaacc
    37741 gtacatccgg cgctggcgat cttcttcacc caattttggc atcagcaaga aatagaggcc
    37801 gatacaaatc accagaatgg gcaaaatctg tcgtaagaca tcagcctgaa cgtattgcac
    37861 cagcagtgcg ccgctcattg agccgacaaa ggtcatggcg atattgagtt tctgatcgct
    37921 taaactaacc actttgcggc gaataaagta gatagtggcg gaaatagagc cgccgcaggc
    37981 ttgcagttta ttggttgcca gcgcattagc gggagacatc cccgccgcca tcaatgccgg
    38041 aatggtgagt aacccaccgc caccggcaat cgagtcgata aatcccgcca gcatggcgac
    38101 aaaaaagaga actcccagca acagcgggga aaccataaac aggctattaa acgtttccat
    38161 tagatcacgt gctcatccag tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc
    38221 ttctcaggct ttgttgttcc cggttttgga ggttcaaacc agctttgcag ttctgccccg
    38281 caaccatcgc ctggtggcgg taaaggttga tcttcacact ccagactatc ggcaggacaa
    38341 cgtaatcgta catgcatatg cgcgcgatgc tggaaccagg gccgcacttt gcgcaaccag
    38401 tcgcgatcgg tgcccgcatc aaagcaaagt tgttgtttaa tcgccggatt aacaaaaatg
    38461 cgtgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc tggcttccac
    38521 agcgtggaga caacgtgttt accgtcgcgg gaaaccaaat ctaacgcttg cgggcgcaaa
    38581 agttgcgcgg aggtccagcg tgttttcggc agttgtaaga agatatcaac atccagtccg
    38641 gtctggtggc tggcatgacc gccgttgaaa cgcccaccag cgggcatccc catatcgcca
    38701 atcagcaccg tacccatgcc cagattgctc acctggctac tcagacgctg aataaacatc
    38761 accagatccg ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg ataatgttcg
    38821 gactgtatcg gcaacgtgtc agcgccgaca atacagccat tagaaaaact gcctatcgat
    38881 tgtgcgctac ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc caggctggcg
    38941 ctactggcaa gcagggccag cagcgcaatc gcggttttgt tcatttttta ccagcgtgga
    39001 atatcagtct tcacatcggc attttgcgcc cgctgccgta acaggtgatc cattaatacg
    39061 atcgccagca tcgcttctgc gatcggcacg gcgcggatcc cgacacaggg atcatgacgg
    39121 cctttggtga tcatctcaac ttcttcgcca aagcggttaa tggtacgccc cggcacggtg
    39181 atactggagg tcggtttcag cgccatatga gcaatgattt gctgcccgct gctgataccg
    39241 ccgagaatgc cgcccgcatg gttgctctgg aaaccgtctt tggtgatttc gtcgcggttc
    39301 tggctgccgc gcagcgccac tacgtcaaaa ccgtcgccaa tttccacgcc tttcaccgcg
    39361 ttgatgctca tcagcgcatg ggcgatgtca gcatccaggc ggtcaaagac cggctcgcca
    39421 agtccggcgg ggacgccact ggcaacaacg gtgactttcg cgccgatgga gtcgccctct
    39481 tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt tgtccgggtc cgggcagaag
    39541 aacggatttt gttcaaccag cgaccagtct ttgatttcca gcggaatgtc gcccatctgg
    39601 gtcaggcagc cgcggatttc aataccaaat ttctcggcga gatatttttt ggcaatcgcc
    39661 cctgccgcca cgcgcatggc ggtttcgcgg gcggaagaac gtccaccgcc gcgataatcg
    39721 cgcagaccgt atttttgttc gtaggtgtaa tcagcatggc ctggacggaa gacgtcttta
    39781 attgcgctgt aatcctgaga acgctggtcg gtattttcga tcaacaagcc aatgctggtg
    39841 ccagtagtaa cgccttcaaa aacaccggag agtattttga cctgatccgg ctcgcggcgc
    39901 tgggtggtat agcgcgatgt cccagggcga cgacgatcaa ggtcatgttg caggtccgct
    39961 tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc agccgagcgc cagcccatgc
    40021 gattcgccga aggtggttac gcgaaagagt tgtccaattg tgtttccagc catcacggct
    40081 ccgttattgt tgtgtttgcg tgtttactta atctttataa atcgcgaaat attcacgggc
    40141 cgccagcagc tgctctttgg tgagcataaa cacaccatcg ccgccgttat caaactccag
    40201 ccaggtgaac ggaacatccg gatattgttc cataagatgt accatgctgt tgccgacttc
    40261 acaaatcaac acgccatcat cagcaaggta atctgccgcg tttccaagaa tgcgacgcgt
    40321 cagtttcagg ccgtcggtgc cagatgccag gcccagttcc ggctcgtggc ggtattcatt
    40381 tggcaggtcg gacatatctt ccgcatcgac atacggcggg ttagtgacaa tcagatcgta
    40441 ctgcactttc ggcaagtcgc ggaacagatc ggaacgaatc ggaatgacgt tgtggatcag
    40501 accgtgttct tcgatgttct gttcggcaac cgccagcgcg tcgggagaga tatctaccgc
    40561 gtcgacttcc gcttccggga aggcataagc acaggcaatg gcgatgcagc cactaccggt
    40621 acacatatct aaaatatgct gcggttgctt gctgataagt ccggcaaatt tattgttgat
    40681 cagttcacca atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa attcatggcc
    40741 gcagaaccac gctttgttgg tcaggtaagc caccggaatg cgttcgttga cgcggcggat
    40801 cacgcgctca acaatgcggt gtttttcgct ggaggtcaga cgcgcggtgc gcatatcttc
    40861 cggaatatcc agcggcaggt agagcgaagg caacaccagt tgcacggctt catcccacgg
    40921 attatcggtg ccgtgaccgt accagatatt tgccgcgctg aagcggctaa ccgaccagcg
    40981 caacatgtcc tgaatggttt gcagctcatt tactgcttca tcaacgaaaa ttttatccac
    41041 gtattcctcc agggcatgat cgcaataatt tcggcggcta gtttgccatg aagatgacga
    41101 taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt tttaaacgat cgattgtgct
    41161 acgagtcggg taaactgtag gaaaattaga aatagagaca gataaatgaa aaagaaaaca
    41221 acacttagcg aggaggacca ggctctgttt cgccagttga tggcggggac tcgcaagatt
    41281 aagcaggaca cgattgtcca ccgaccgcag cgtaaaaaaa tcagcgaagt gccggtgaaa
    41341 cgcttgatcc aggagcaggc tgatgccagc cattattttt ccgatgagtt tcagccgtta
    41401 ttaaataccg aaggtccggt gaaatatgtt cgcccggatg tcagccattt tgaggcgaag
    41461 aaactgcgcc gcggcgatta ttcaccggaa ttgtttttgg atttacacgg tctgacgcag
    41521 ctgcaagcta agcaggaact gggcgcgttg attgccgcct gccgccgtga acatgtgttt
    41581 tgcgcctgcg tgatgcatgg gcacgggaag catattttga agcaacaaac accgctgtgg
    41641 ctggcacaac atccgcatgt gatggccttt catcaggcac caaaagagta tggtggtgat
    41701 gcggcgttgc tggtattgat tgaagtggaa gagtggctgc cgccggagtt accctgagaa
    41761 gtttagcgcg ttgcgccatg tacggggaat ggcagggtca aatgaccctg ccttgtgaaa
    41821 aagacgcact agtacatatt cagtatgtaa gtgaaccggc aggtggcatc tattggctgt
    41881 tgcgccgttg tgtccacgcg tagcttcata tcatgcagtt gcctggacgt gatcggttgc
    41941 ggctctggcg tcactgtcac tttggcatcg tcacagcccg aagtggagaa ggtcatctgc
    42001 tgtgcgacaa aacggcagta agcaccacgc tcgtcatatt tactaccatc acacagtttt
    42061 ccaccttcca tcagataagt gggcagcgtt tgttcaatca cagcacctgt tgattgcatc
    42121 ctgactattt tgttatgcac gaccgtattt tcattccagg agacatcata aggatctaaa
    42181 ggtattgcgc tacagtcaga actgactttt gtcgggccaa tatagggaac attattacct
    42241 gtatatattg cctccgttgc gtcctcactc tttacatacg aggaaactat ccacggacaa
    42301 ccatgcatat caccgtcata gaaaactgtg actttcattt ccacgcaggc ccaggtcgtt
    42361 tgtggggtgc gagcatcata ttcttcaagg cccgggcaac gtgaagttgg taatatggtt
    42421 cttgcgtctt ctaaatgcag ttccggagca gtaccttgac cagtccatga tactagtggc
    42481 acattcaaaa tcgcaactga cctggattga tataccccat attgcgcgcc ttctggtgtg
    42541 aggatagaaa gattaaactg aaaacctgtg agcactccca ttgatcccgc aggaatgtat
    42601 aacgtcgcga tctttttcgc ggcctgcact cctgtgctga ggcaaatgag cagcaaaaag
    42661 taaaagacat aacgtagaat tttcatttta aatatactcg aaggtagcgg tcaggctggc
    42721 agtgaattca ccgatcgtca catcgcgacc gcttttcgcc tggatccagg cccggaaatt
    42781 aatatcattg ttgccgttgg taagcgcaaa agtcgccccg gtagtgttgt taatatccac
    42841 ccgcttgccg tcgagcgttt ccatccctat ccccacgccc tgcgcggtgg aggtgtcatc
    42901 gactgccaga aaacccggct gaccgctgtc ttcggtgccg ctcagcgtga ctttgacgtt
    42961 gtagcccgct ggccctttgc agtctttcag cttaaacaca accggcagat gcacagactg
    43021 tccggcgact atcaaatccc ggttgctgac ggtgggaaaa tggatttctg ccagattggc
    43081 accatccacc accagcgtgc aggatttact cagcaggttg ccgtaaaaat gcaggttgtt
    43141 atcgactgcc cgtgcccccg tgctggcggt cagcagcagg cctgctaaca gcgcataacc
    43201 gggtgaataa atgtgtgctg ttttcattgg taatccaccc gtaaggttgc gtaagcagaa
    43261 aaggtcgtgt ccgtcagcac cgcatcagcc gctttgaccg gtaccgcctc cagctttggc
    43321 ttctggctaa aatcggtggc gttaatggaa agcggctcgt tgagcttaaa acgctgaccg
    43381 tcatgctgaa gctcaattcc gaagcccggt acatccgtct caatagcaga gtcgttgtag
    43441 gaggtggcag tccccgtcca tgagagtgtc atattccact cgggatcgcg gacatcagac
    43501 tcacagctca gttcgtagtc cacttcctga cgaccataat tgccgtcaat gctgtcgata
    43561 atgagatcgc gaaattgcac ttcgatggtt tgcccgccgc tgatgctaca ggcgggcggc
    43621 gacatcagcg taccgtggaa tgtgatgtca tcttccgccg ccagcacagc cccgcaacca
    43681 gcgcacagta aaaggagcag gaattttttc attggtaatc caccaccatg gtcatcgtgg
    43741 cgttaaactc cgacggcgtc agttgcaccc cgctctgttt taccggcacc gctttcagct
    43801 gaggctgtgt gttgacatta aaaggcgtcc agttattttc accaactgca aacagggagt
    43861 tgtcggcggc gttttctatc cgcaccccca ggccggcgat atccgtgctg agaaccgttt
    43921 ctccgttgat cacggcggta ttgcctttga gctgcatccg taagtcatcg ctgactttat
    43981 taccgcagtt gagggtgtat ttcgcatcct gttggtaatt ggtaccatcg atcctgttga
    44041 tcaccacatt gccaaattcc accgcgcttc ccttcacgga gcacggcggc gggccatcga
    44101 ccaggattcg cagcgtcaga tccacctgtt tgatatcgct gccagcaacg gctaaaccgc
    44161 tgacacccca tccggccagt accagtagcc ccgcgcccgc taaaaacttg tgactcataa
    44221 gtccctcccg gcatcagcct ttgcggctct gctcttcatc cacctggcag ttgttgccgg
    44281 tgcatttgaa gtacagcggc atccgcgcgc cgtagtcgtt gatgtaggtc agcaccggca
    44341 cgctgtccat cttcgcattc agcggtgcgg aggttttcgg ctccatcatc agcggtgtga
    44401 atcccgccgg cgggttgccg ccttttttag cgctggcgtt gctgatgatc acgtagtacg
    44461 gcgtcgggtt attgacggtg aagtcctggc cgttacggat gagcgtcacc ttatgctgcc
    44521 acggactttt catgctggtg ttctccagcg ccttcggtcg ccagaacagt ttgatacgcg
    44581 tctgcagcgc aatctgcagt gtgttcggtt tatttgattt cggcggaatt tcgcgcacgt
    44641 tgaaatagaa cacgctctcg cggtcggccg gcagtttgtt gatatccggc agcccctgca
    44701 ccttcacctg gccgttcatc atcgcatcga tgcgctgcac tggcggaagc accgcaagcg
    44761 gcgagctgat tttgttgccc tttgcatcct ctatccagct ctgcgccaga taaggcagtt
    44821 tgggatcgtt gttgcgcagc gtgacgctga tggacttgtc gctttcgtta aacaccagcc
    44881 gtgtcctgtc gggcgtcacc gaagcgtggc tggagaaact gaccgctgtc gccagcagcg
    44941 ccagtgccgg gacaacgatt tttgcagtgt tagtcagatt aaacatggat cattccttaa
    45001 tcacgttact ggtttactga aattgaagtc gggggttccg ttggcgtcac ccgctgtgtc
    45061 tgttcctgaa tcgccggttt gaccgccggc gaagtgacag gtgctacatt gcctttatgc
    45121 tggcatggca gtaacaagcc gttaaacaga tcggcaggca gcgggtccgg caggttgata
    45181 tcgcaatgcg caacaccact ccagaacaca ctcatgtgtt cgccaggttt caccccagcc
    45241 agataaacat tgccgtcatc atcgacaagg cccactgtct gctcgttatc attttttact
    45301 tctgcgccaa acggaggatg gctgccgtct tgtaaacgca gcaccgccat cgctttttga
    45361 ccactgatga cggcaaattt gcggtagcca atcgcccctt cagttagcgt ggcttgtacc
    45421 accgactggg ttgcttcggc gttttccggc agtttgttga ggtcgatata cgcctgattg
    45481 cggtaatagt tattgacgtc agaaacgacg gctttaccaa acatattggt ataaacagca
    45541 gcaccgttac cttctaccgg aacatcggca acgccatcgg catcaatcaa caagcgtgtc
    45601 ccgcccatat tctgggtacg gtgaagtgcg ccaccgtggg cagtaagcgt tgcgccgccc
    45661 tgtaacgaca agcccgcaga ggtgtattgc ccttcatggt agttcgcact gaggtcaacc
    45721 tgcgccagcg aaccatcatg gctgtaatag ccgtcaacgc tggtgtgttt gtcactggtg
    45781 ccgacgttca actgatagtg agtcgcgtca tcgacacggc tgaaataacc gacctgactg
    45841 ctgtccgtcc cactgccata gttaccgtta tagctaacgg tactgttgtc gccccacggc
    45901 atactgagcg aaatgtacat gcctttgtcg gcctggttgt catactcgta acggtagcca
    45961 gtcatcgaga tgctgacatt acgaatactg cccatattga aatagtgcga gagcatgatg
    46021 ttgtagtttg tctgctcctc gcgatcccag taggtatggc gggtatagtt gagataaacc
    46081 gaaacacccg catcgcggaa gttctggtta taagtggcgg tgtacatctc tttgtcgttg
    46141 cccgtgcgta ccattccgct gtcactggca tccagatact cgctcatggt cataaagttc
    46201 tcttccgaga agcgatatcc agcaaaagta acgcggctgt tgagctggtc aaagtctttg
    46261 gaataactca cacggaagga gttaccgtcc agcgaacctt tgccataagc ggtgtcttta
    46321 tccagtttgg tgtgcgagtg agtaacatca aacgcgaccg cgccgaatgt agacaaatcg
    46381 cgaccgacgc caagcgccgc agactgatag ttttcatctc ccagtgcgcc gccatataac
    46441 gaccagccgt tagcgatccc ccaggaagct tcagcaccag aaaaaaattc accctcgaca
    46501 tggtgtcccc actcttgcgg acggcccatc atgatcttat agcgaacctg acctggacga
    46561 gtgaggtatg gcatcgaggc ggtgctgatg tcatattcct gcacctggcc gttctgttct
    46621 tcaatgcgaa tatgcaacgt accggagaca gaatcaccaa gatcctgaat acgaaacggt
    46681 ccagccggaa cctgcgtttc gtaaatcaca cgccccatct ggctgacggt cacttttgct
    46741 gtggtgtgtg ccacgccgga aatgtctggc gcgtagccgc gcagattggg aggcaacatt
    46801 tgatcgtcag tactgacact gccaccaaca tagttaaaac catcaaaaat atcggatctg
    46861 aggtaatcct cgcccagcgc cagtttggct tttaatgatg gtaacgcccg ccaggcatag
    46921 tagcgactcc actcccattt tttttgagtt tcatcgccgc tgaattcatc gtcatcatta
    46981 ctgcgagtat gttgatagtt agtctgccag tcagcacgca tacgccacgg ccccaggtta
    47041 accccgaccg tcccgttgcc gctgatctcg ttactatcat caccgccatt ttcttcgtgc
    47101 cgggtttgtg cgttgatgct gtagtccgcg acgatcccgg agatgccgtc atcccaacgt
    47161 gaaggcggat cccagtcggg ataagtatat tcgaggtaag cctgtggcag tgaaatgact
    47221 aatgcggact ggcttaaatc agccttaatt tccacgcctt ccagttgacc ggatttcagg
    47281 catttagcat cgtggctcca ttgcagattt ttcgccacgt cttcttttaa accaaactgc
    47341 gctaccagtt ccggtgtcag acaagcatag cttttgctcg cgtcatcttc accagcatac
    47401 cagtaaatat cgtactcttc cgccaatggc tgtttattta gttgaacctg taaattgtat
    47461 tttccgggct caagatatcc ctggctggaa aaacgcttca gatcaatttt tgtgtcgcct
    47521 tttaattcca gaaaacggga atcaaactga atgtcgtctt cagcccagac actgctataa
    47581 ctccctgaca ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa aagagaatga
    47641 tcaggcatac acgtttctat ccgtttttat tcttgttcgg tcaaaatcca tttaaatccc
    47701 ccatagcagt gtcgctatgg gggtatgcct tacaggtaag taatctggaa ggtggtattt
    47761 gcttcgaaat tacccagatc tggcgcatca gctgcgccga ccagccaggc tttaaagttc
    47821 agtgtctgct tcgctttgcc tttattagta gaagtgtctt tcactatttt ctggtcaata
    47881 cccatgccgc ttttgtaaga ggtacccaga gagtcaccga tcgccaggct aacattgtta
    47941 aatgccgcac cggtatcggt attgaaaatg gtgtaataat tgccgctatt tgcagaagaa
    48001 acggtaccag taaaggtagt ggtcattgtt tcctgagtat caaatacgca atcttgcaga
    48061 cgaatctgga aacttttcgg cgtggtggtg ccgttatttt tcagtttttc ggcaccgata
    48121 tcacccatat caacttccag tttgtgatcg tccggaacga tagaacacac tgagtcttca
    48181 atggtgccgt aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc cgtagagg
//
LOCUS       DAJOSY010000021        45430 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJOSY010000021 DAJOSY010000000
VERSION     DAJOSY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.021"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            45..317
                     /locus_tag="OHN27_001314"
     CDS             45..317
                     /locus_tag="OHN27_001314"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285794.1"
                     /translation="MNWKLIVTAIIGFLSFCIFLSITLLIPDLNEFQKKMIFSLYFMS
                     AAVGISPFIANNIKVNGKMAFRGSEFKVSAVGGLAALIVSFIIQVI"
     gene            complement(344..685)
                     /locus_tag="OHN27_001315"
     CDS             complement(344..685)
                     /locus_tag="OHN27_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCQ2285795.1"
                     /translation="MRDMYEVLDRWGAWAAADNSSVDWQPIAAGFKGLLPHGKKTRLQ
                     CDDDEGIMIDGCVARLRKYKQEEYELIIAHFVIGISLRTIAKKRKCSDGTIRKDMQTA
                     LGFVSGVLSML"
     gene            complement(703..1692)
                     /locus_tag="OHN27_001316"
     CDS             complement(703..1692)
                     /locus_tag="OHN27_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCQ2285796.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(1700..2497)
                     /locus_tag="OHN27_001317"
     CDS             complement(1700..2497)
                     /locus_tag="OHN27_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCQ2285797.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(2517..2906)
                     /locus_tag="OHN27_001318"
     CDS             complement(2517..2906)
                     /locus_tag="OHN27_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCQ2285798.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPAAVEIVLFPPDNRIRDLDNYNKALFDSLTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(2903..3229)
                     /locus_tag="OHN27_001319"
     CDS             complement(2903..3229)
                     /locus_tag="OHN27_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCQ2285799.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLVGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(3226..3879)
                     /locus_tag="OHN27_001320"
     CDS             complement(3226..3879)
                     /locus_tag="OHN27_001320"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCQ2285800.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(3879..4373)
                     /locus_tag="OHN27_001321"
     CDS             complement(3879..4373)
                     /locus_tag="OHN27_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCQ2285801.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEAEPQPVRETRPVRNFYVGTNDPREILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(4370..5341)
                     /locus_tag="OHN27_001322"
     CDS             complement(4370..5341)
                     /locus_tag="OHN27_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2285802.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAALSQLS
                     DSDPSKSDTSKSDTSKSDTSKSDSSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDK
                     KPSRPDASQPDTQKAEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQ
                     AAECDGEVVRPKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPS
                     KLREKWDELSLRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(5331..5510)
                     /locus_tag="OHN27_001323"
     CDS             complement(5331..5510)
                     /locus_tag="OHN27_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCQ2285803.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(5686..6237)
                     /gene="ymfL"
                     /locus_tag="OHN27_001324"
     CDS             complement(5686..6237)
                     /gene="ymfL"
                     /locus_tag="OHN27_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HCQ2285804.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(6230..6490)
                     /locus_tag="OHN27_001325"
     CDS             complement(6230..6490)
                     /locus_tag="OHN27_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2285805.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            6588..7280
                     /locus_tag="OHN27_001326"
     CDS             6588..7280
                     /locus_tag="OHN27_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2285806.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            complement(7603..8100)
                     /locus_tag="OHN27_001327"
     CDS             complement(7603..8100)
                     /locus_tag="OHN27_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285807.1"
                     /translation="MAAIKETAGLAKVINDAKTDAEVKAATIELQNKLITLQAECFSL
                     GDAIRLRDEEVMHLKAKIAEFEDFCAKVEGYVLDQLDSGAFVYSKNEIVSGKEITVHL
                     CPLCYSKNIKSILHPLPVGKTSHFLTSRCLHCENKFLMEKNPMYERPRSLRELGRDLN
                     SPWIP"
     gene            8269..8579
                     /locus_tag="OHN27_001328"
                     /pseudo
     CDS             8269..8579
                     /locus_tag="OHN27_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8594..8956
                     /locus_tag="OHN27_001329"
     CDS             8594..8956
                     /locus_tag="OHN27_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285808.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGR"
     gene            9022..9846
                     /locus_tag="OHN27_001330"
     CDS             9022..9846
                     /locus_tag="OHN27_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HCQ2285809.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFGANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            9974..10510
                     /gene="yfdR"
                     /locus_tag="OHN27_001331"
     CDS             9974..10510
                     /gene="yfdR"
                     /locus_tag="OHN27_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCQ2285810.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLESIPATEMFNVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            10501..10863
                     /locus_tag="OHN27_001332"
     CDS             10501..10863
                     /locus_tag="OHN27_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285811.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            10863..11168
                     /locus_tag="OHN27_001333"
     CDS             10863..11168
                     /locus_tag="OHN27_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285812.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            11395..12558
                     /locus_tag="OHN27_001334"
     CDS             11395..12558
                     /locus_tag="OHN27_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCQ2285813.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWRLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            complement(12573..12648)
                     /locus_tag="OHN27_001335"
     tRNA            complement(12573..12648)
                     /locus_tag="OHN27_001335"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12613..12615),aa:Thr,seq:cgt)
     gene            complement(12763..14016)
                     /gene="proA"
                     /locus_tag="OHN27_001336"
     CDS             complement(12763..14016)
                     /gene="proA"
                     /locus_tag="OHN27_001336"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCQ2285814.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLLALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(14028..15131)
                     /gene="proB"
                     /locus_tag="OHN27_001337"
     CDS             complement(14028..15131)
                     /gene="proB"
                     /locus_tag="OHN27_001337"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCQ2285815.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            15419..16474
                     /gene="phoE"
                     /locus_tag="OHN27_001338"
     CDS             15419..16474
                     /gene="phoE"
                     /locus_tag="OHN27_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCQ2285816.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(16513..16914)
                     /gene="crl"
                     /locus_tag="OHN27_001339"
     CDS             complement(16513..16914)
                     /gene="crl"
                     /locus_tag="OHN27_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCQ2285817.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(16972..18216)
                     /gene="frsA"
                     /locus_tag="OHN27_001340"
     CDS             complement(16972..18216)
                     /gene="frsA"
                     /locus_tag="OHN27_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCQ2285818.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(18308..18766)
                     /gene="gpt"
                     /locus_tag="OHN27_001341"
     CDS             complement(18308..18766)
                     /gene="gpt"
                     /locus_tag="OHN27_001341"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCQ2285819.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            19027..20484
                     /gene="pepD"
                     /locus_tag="OHN27_001342"
     CDS             19027..20484
                     /gene="pepD"
                     /locus_tag="OHN27_001342"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCQ2285820.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(20541..21062)
                     /locus_tag="OHN27_001343"
                     /pseudo
     CDS             complement(20541..21062)
                     /locus_tag="OHN27_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(21061..21264)
                     /locus_tag="OHN27_001344"
                     /pseudo
     CDS             complement(21061..21264)
                     /locus_tag="OHN27_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            complement(21510..21962)
                     /locus_tag="OHN27_001345"
     CDS             complement(21510..21962)
                     /locus_tag="OHN27_001345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2285821.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMISSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITCVERYIDMLFVDPEYTRRGVACALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQY"
     gene            complement(21972..22370)
                     /gene="yafO"
                     /locus_tag="OHN27_001346"
     CDS             complement(21972..22370)
                     /gene="yafO"
                     /locus_tag="OHN27_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCQ2285822.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKQARDNNQMHKIGKMAEAFRMRF"
     gene            complement(22373..22666)
                     /gene="yafN"
                     /locus_tag="OHN27_001347"
     CDS             complement(22373..22666)
                     /gene="yafN"
                     /locus_tag="OHN27_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCQ2285823.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(22718..23773)
                     /gene="dinB"
                     /locus_tag="OHN27_001348"
     CDS             complement(22718..23773)
                     /gene="dinB"
                     /locus_tag="OHN27_001348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCQ2285824.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(23844..24767)
                     /gene="lafU"
                     /locus_tag="OHN27_001349"
     CDS             complement(23844..24767)
                     /gene="lafU"
                     /locus_tag="OHN27_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCQ2285825.1"
                     /translation="MRKTGNRRDRGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(24770..25633)
                     /gene="motA"
                     /locus_tag="OHN27_001350"
     CDS             complement(24770..25633)
                     /gene="motA"
                     /locus_tag="OHN27_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCQ2285826.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(25646..26362)
                     /locus_tag="OHN27_001351"
     CDS             complement(25646..26362)
                     /locus_tag="OHN27_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HCQ2285827.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGILLAGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(26382..26849)
                     /locus_tag="OHN27_001352"
     CDS             complement(26382..26849)
                     /locus_tag="OHN27_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HCQ2285828.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(26857..27918)
                     /locus_tag="OHN27_001353"
     CDS             complement(26857..27918)
                     /locus_tag="OHN27_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HCQ2285829.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATHPQENDPVAMQALMALLLPHPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTPLPPQLQELISQLPQEKPEQQARLATYASEDLHAIASTQ
                     PRVSTQPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPVTLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(27915..28226)
                     /locus_tag="OHN27_001354"
     CDS             complement(27915..28226)
                     /locus_tag="OHN27_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HCQ2285830.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(28231..28623)
                     /gene="fliS"
                     /locus_tag="OHN27_001355"
     CDS             complement(28231..28623)
                     /gene="fliS"
                     /locus_tag="OHN27_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCQ2285831.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(28646..29962)
                     /gene="fliD"
                     /locus_tag="OHN27_001356"
     CDS             complement(28646..29962)
                     /gene="fliD"
                     /locus_tag="OHN27_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCQ2285832.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQSELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPAITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(30214..31128)
                     /gene="lafA"
                     /locus_tag="OHN27_001357"
     CDS             complement(30214..31128)
                     /gene="lafA"
                     /locus_tag="OHN27_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HCQ2285833.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            31614..32456
                     /locus_tag="OHN27_001358"
     CDS             31614..32456
                     /locus_tag="OHN27_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCQ2285834.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPESSPAEALIITNQIQETVPGEISSTTPPVPVRKKHKGLMGLALTAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLSS
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(32511..33488)
                     /locus_tag="OHN27_001359"
     CDS             complement(32511..33488)
                     /locus_tag="OHN27_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HCQ2285835.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVSFHSPDLVNLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDNAGQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPNIERTLSVRSDGDK
                     SAFMPLKIFAEPSQADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(33505..34434)
                     /gene="flgL"
                     /locus_tag="OHN27_001360"
     CDS             complement(33505..34434)
                     /gene="flgL"
                     /locus_tag="OHN27_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCQ2285836.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQAPNVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(34449..35825)
                     /gene="flgK"
                     /locus_tag="OHN27_001361"
     CDS             complement(34449..35825)
                     /gene="flgK"
                     /locus_tag="OHN27_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCQ2285837.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTPTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(36008..36307)
                     /locus_tag="OHN27_001362"
     CDS             complement(36008..36307)
                     /locus_tag="OHN27_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HCQ2285838.1"
                     /translation="MKINGSGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(36307..37407)
                     /locus_tag="OHN27_001363"
     CDS             complement(36307..37407)
                     /locus_tag="OHN27_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCQ2285839.1"
                     /translation="MQNWIKTVVVAVSLALPGVVLAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINSSFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(37422..38087)
                     /gene="flgH"
                     /locus_tag="OHN27_001364"
     CDS             complement(37422..38087)
                     /gene="flgH"
                     /locus_tag="OHN27_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCQ2285840.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(38291..39076)
                     /gene="flgG"
                     /locus_tag="OHN27_001365"
     CDS             complement(38291..39076)
                     /gene="flgG"
                     /locus_tag="OHN27_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCQ2285841.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDATELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(39155..39892)
                     /locus_tag="OHN27_001366"
     CDS             complement(39155..39892)
                     /locus_tag="OHN27_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HCQ2285842.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATNN
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNPEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(39892..41094)
                     /gene="flgE"
                     /locus_tag="OHN27_001367"
     CDS             complement(39892..41094)
                     /gene="flgE"
                     /locus_tag="OHN27_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FlgE"
                     /protein_id="HCQ2285843.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLISTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGDYLQGYPVDDSGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     AAIDRTTVPFDPTNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLIFDPNTGKLTSPTTPQTIEFQTDAAAPIDLTVDYTTCTQYGSDFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(41254..41967)
                     /locus_tag="OHN27_001368"
     CDS             complement(41254..41967)
                     /locus_tag="OHN27_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HCQ2285844.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPQGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(41967..42398)
                     /gene="flgC"
                     /locus_tag="OHN27_001369"
     CDS             complement(41967..42398)
                     /gene="flgC"
                     /locus_tag="OHN27_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCQ2285845.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(42401..42736)
                     /gene="flgB"
                     /locus_tag="OHN27_001370"
     CDS             complement(42401..42736)
                     /gene="flgB"
                     /locus_tag="OHN27_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005314272.1"
                     /note="with FlgF and C makes up the proximal portion of
                     the flagellar basal body rod; Yersinia has 2 copies of
                     flgB and other flagellar genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCQ2285846.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAVVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            42818..43555
                     /gene="flgA"
                     /locus_tag="OHN27_001371"
     CDS             42818..43555
                     /gene="flgA"
                     /locus_tag="OHN27_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCQ2285847.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDTPLTANDVELKPYNVSAQRRDVLMVLDDAI
                     GFSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            43636..43914
                     /gene="flgM"
                     /locus_tag="OHN27_001372"
     CDS             43636..43914
                     /gene="flgM"
                     /locus_tag="OHN27_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HCQ2285848.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTATTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSLQVDTEQLASDMLSFFQN"
     gene            43927..44355
                     /locus_tag="OHN27_001373"
     CDS             43927..44355
                     /locus_tag="OHN27_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HCQ2285849.1"
                     /translation="MSIRLQQVKALLQGIRADDALYDSLRELLQRQRICMIRRASEEL
                     LAVNEEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFAWLPAVQKAAAQQLWQR
                     LEQKAERCKTYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            44482..45411
                     /locus_tag="OHN27_001374"
     CDS             44482..45411
                     /locus_tag="OHN27_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HCQ2285850.1"
                     /translation="MSFIECYEPEYVRKFLARHPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEENAAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSRASKMPGRDGDILARLTTLPQALADHAKKGILQAQFAQLPPVPQLARHLATLLG
                     SFTFDWSILPESPRKTSLPLQMPLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDVIGQTDGADYFPLVCDFYLLRTLISLWTLDGSSLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
BASE COUNT        10580 a        12141 c        11490 g        11219 t
ORIGIN      
        1 agtctcgaaa tttgccaatc gatggattta ctataggtac accaatgaat tggaaattaa
       61 ttgtcactgc cattattgga tttttaagtt tttgtatttt tttatcaatt acgctgctaa
      121 taccagattt gaatgaattt caaaagaaaa tgattttctc tttatatttc atgagtgcgg
      181 ccgttggaat ctcgccattc attgcaaata atataaaggt caatgggaag atggcatttc
      241 gtggttcgga attcaaagta tctgctgttg gtggtttagc agcattgatt gtttctttca
      301 tcatccaagt aatttagcaa agaggcccta aggcctctta taatcatagc attgatagca
      361 caccactaac aaaacctaat gctgtttgca tatcttttct aattgtccca tcagaacact
      421 tacgcttctt cgcaatggtg cgtaatgaaa taccaataac aaagtgggca ataattaact
      481 catattcttc ttgtttatat ttacgtaacc gtgcgacaca accgtctatc atgatccctt
      541 catcatcatc acactggaga cgtgtttttt taccatgagg taaaagcccc ttgaagcctg
      601 ctgctatcgg ctgccaatcc acactactgt tatctgctgc agcccatgct ccccagcggt
      661 ccaaaacttc atacatgtca cgcatcaact ttctccacaa aatcaggcca gcacgccaat
      721 tgccagcgca cgatcgataa aacgaaatat caactccagc tgggagccat acttctcttc
      781 aaatgccacg gtatccgcat gcagctcgtc gtgatgcttt ctgcacaaag gcaacacaaa
      841 gaggtcatgc gcttttgtac ccatcccgcc ctgaccgtgg cctatcaggt ggtggggatc
      901 atcagcaggc tttccacaac atgcacacgg ctgcgtctta acccagcgcg tgtacttttc
      961 gttaacccag cggcgacgtt ttgggcgtaa cataaaagac tccggcgact ccggatccac
     1021 tttcagcgcc agcacctttt tcgctttatc ctggatgatg ctggtggcag gaaccgaagg
     1081 cacaaggtca ctttcccggg tgacagacgg cacaacaggc ttcggtaatc tcagtgcctt
     1141 acgggctgca ctttccggta aggcatccgc caggtcatta cgaaccagcc accagcacag
     1201 ttccggcatt gtcacaacgt gactatcatc aaaaccgaga tcccgacgca cgacagacaa
     1261 cacccagcgg gcacagttat ccgttgccat tgattccagc cgttccgtga actgatcgcg
     1321 cagctggtta tcgcagtgcc agcacagacg gattgcgccc ggcgcgtgtc gcattgtggt
     1381 catgttctcg ctgtgccagt cggaatgagg ccactggcag cctttttcac gaagtaacca
     1441 gctttcaaga cattccactc caccagcacg acggatcact gcctcattgc ggaacacggc
     1501 ccgaacggca ggatcatccg ccagcggttg tgatgccgcc ggaacggcac cactggcgaa
     1561 agatgaataa cgttccggct caggctccag caggacacgc ccctgcataa acaagggcat
     1621 cagctctgaa cctggtctga acaatacgat ccccatacgc ggggcaattt caggggtcag
     1681 tagtgctctc acggtcacct caatgaacgg tatcgagcag ctttaacagc tcagggaatc
     1741 gggattcgaa gaaatgcggc tgcgtctcgc gcggatttgc gggactggta atgttcttgc
     1801 cgaacatgca gcctttcgcc gttagcgacc agaatttttt gatgttgtta atcgcggtac
     1861 ggctgtatcg ttcgcgctgt tcgacgatcc ccagtttcac catctggtga tatgcctgat
     1921 tagctgtcag gcggatacca tactgcttca gcagtgcact cagtgacagc gtggggcggc
     1981 ttgagccatc aggcgcgtca gcaggagcat caatggcata gcgcggtgcc agattcggta
     2041 agccaacagc ctcctggagt ttctgacagg caccaagcac tgaagagtta gacaggttta
     2101 actccctgcg cataaagtcc agcagaatca caccagcctg catcttgtca gcagcctgcc
     2161 cggataattt ttccggtgcg ctggttacca tatcgaaagt acggatcacc ttcagatgga
     2221 atgacgggct gatccacatt gcataggcat acaccagttc tttgcagaca tacgtcccct
     2281 ggttatttcc gccacgaata acgttaactg gctctatatt gaccgagttg caaatctgca
     2341 actcgcttat taaacgttca gtttgctcat tgcggagcca gaatgcaggc ttatgcttat
     2401 ccagagaacc ggcagccctg tgcagatcgt tcaggctgta acgaccataa gcatcacgac
     2461 gaacttcaat accatcaatg accatcagat tattcatact tcgtttctcc tcttaatcag
     2521 gcggctgcac ccgccgtttt ctcgtactta ctgatagtga tctcgacctt cccttccggg
     2581 ataaccggtc cccactccac cagcattctt ttcacctgac tgtcgtcttc ccacacaccc
     2641 gcgtgggtca gggagtcaaa cagcgccttg ttatagttgt ccagatcgcg gatccggtta
     2701 tccggtggaa acaacacgat ctccactgca gcaggtgccg acgttggttt cggcagacga
     2761 cgtaactgct caactattgc tgcgcacgcc gcgctctgga attttcgccc cgccgcgctt
     2821 atcagactct taccagcaaa cgcccctttg ttggggtgtc gccagtacgt gttcacgctg
     2881 ggcgggaaag gcaggatcag cttcatactt tcaggcccct ctcatgtaac cagtgggctg
     2941 cacgcagcct ggcgttttcc tcaccgacaa gcagtgagcg gataatcccg accgcctcgc
     3001 tgtcgtcgtc cttcaccgcg gtatgaagcg tgatgccccg ggccacgcca cgctttatcg
     3061 tgatgacgcc ttttttctcc agtgcgcgaa gatgttctac cgctgcattc accgaacggt
     3121 atcccagcat ggttgccacc tcctgattgg ttggcgggaa gccacgttct ttctgataag
     3181 aaatcagcat atccagcacc tgctgctggc attgagttaa cgtcgtcatg ccgccatctc
     3241 cctgaccagt ttttccgcct gctggcgaac ctgcgccaga aacgcctcac cacatgcctc
     3301 aagttcatcg cgcccgatgt agctgattgc cggtcccttc caggtcttgt caaaaacagc
     3361 aatagcacca gcgaaaaaag ctcctgtcgg tacctgcttc tcgtctttcg ggataaacca
     3421 gacaggcagt tcaaaaccaa tacgcccgcg aataaaagca atatgatctg catcttccgg
     3481 ccaccacact tcgctggtgg cagctttgat caggaaaaca tagcgcccgc ccttatcacg
     3541 catggcactg gcatgtttca tgatgtaacg catgccggtg atgtattgcc cctcatgctg
     3601 actggcgcgg ctgtatgggg gattaccaaa ggcagcacct ttaagctccg caagacgttc
     3661 tgaccagtca tgcgccagcg cgttatcttc cgccgtgtaa tacgcggcac atttggcgtt
     3721 atcaccgtca gtaaacagat ccagaacaaa cgggccaaac agggtgttaa ttccccagaa
     3781 aatgttgtcc ggcgtgcgcc actgatcgcc cacttccttc agttcatggg ctggtttgtt
     3841 ccgcagttcc accagcgcct ggcaatattt attactcatt aagcccccac gtaattccct
     3901 gacagatacc actcttcacc cgatgcagcg cgcttgctgc ttttccgtaa gcaccgctca
     3961 cgacgcgcca gaaaattgtt tcgttctggc tgggagtggc tttcacggaa tgccgccatc
     4021 cacacggttg cagcacgacg gtataagccc ctggactcca gttcttcagc ctggcgggtc
     4081 aggcacaaaa tctcccgcgg gtcgttagtg ccgacataga aattgcgcac aggtctggtt
     4141 tcacgaactg gttgcggttc cgcctcctgc gctctctcag tcaggcgcgg gaaatgtctg
     4201 cgtgtatccc cttcacaacg gtgagccaca cgaccactct gacgtaactt gcttgctgac
     4261 tgcagaacgc gctgccgtga gtaacctgca aaagcatccg caatgtctcc ggaagtacac
     4321 cccggatggg cttcaatgaa tttctgaacg tcattcaaaa gactcataat caccccctga
     4381 atcctgccgg gatctggctg tagtccacgt tgtcgtaact ggctttgaag tacgggtctt
     4441 cgcgtttttc ggtgtacgtg cttacggacg gcgataagcg cagggaaagc tcatcccatt
     4501 tttcccgcag cttcgacggg ctaagcacgt tacggcacca gaacggatcg cggctgacgc
     4561 ggctgtacat ctcgcagatt tgtttgtgag tacgaccatc ctgcacacac atcaggcgaa
     4621 tttcgtttgc ccaggctgtc cagttcggtt ctttgggacg aaccacctcg ccgtcacatt
     4681 cggcggcctg ctcgtacagg gcgatgattt ttttccagag ccactgtgcg caggtcaaat
     4741 catcctgcgt tccccactgg cgctttttag ggctgaatac aaccgcatca ggatggcgag
     4801 ttaaaaaatc ctgttcagcc ttctgcgtgt ccggttgcga agcgtccgga cgagaaggtt
     4861 ttttatctga cggatcatgt tttgatttta ctgacggatc cccgccagat tctgacgggt
     4921 gaaaacccgc ttttttgcca gatttcgacg catcaaattt tgacgagtca gattttgatg
     4981 tgtcagattt tgatgtgtca gattttgatg tgtcagattt tgacgggtca gaatctgaca
     5041 gttgagaaag tgccgcagcc tgaagctttg caacgttaag ctgatagacg ttcgacgcat
     5101 tgcggttacc ctggcgacgc gccttacgcg ttaaccagcc ttctgcttcc agccgtgcga
     5161 tagccgttct gacggtactc atccccgcgc caatctgacg ggcaatggtt tcaattgatg
     5221 gccagcacac accttcgtca ttactgaaat cagccaggcg tgccataatt gccacgctgg
     5281 ataatttcat gcctgacgct gcgcaaccat cccatacata gccggttaat ttagtgctca
     5341 tgaccgacct ctatttccct gaatttacga cgaaactgtt cgagcggact gaagcattca
     5401 tgctcatagc cttcgcggag gtagataacc cgttgtgttt ccggctccca acgaatgact
     5461 ctgacgggta ctccgtagtg atctttgaac cagcggttaa cttgtcgcaa aggactgtct
     5521 ccttttgccg gttgaaatca cccacagccc actctgcaaa gctgtgggtt acaattaccc
     5581 tgccacctgg tacatttact gcatagcaat actccacctt cgcttttcca cccggtacag
     5641 gaagcgcaat cagttgcgag cgacggtagt gtgttgttaa actgttcatg cgttagtttc
     5701 tccacagtca cgacacgcca cggcgcccgg agctgcacac tcgcgggcgt cattactttc
     5761 tgaaatgcaa aaaattttgt agaccagtgc tgcatgctcc tgcagcttcg aaattgagag
     5821 gtacagctcg tcgttaattg ctgtcttctc atgcggttcc actacaccgt cttcaattgc
     5881 tgaacgaatc tgttttgaat aactgccgat ctgttcaatg acctccagca ggcgttggtt
     5941 gatatcggcg ttgtccacat cctcgacatc aggaagagac acaaagacgc catttgcaga
     6001 ctgcgccaca gcgtcagcaa tgaagtgagt tccaccagca cgttgcaaaa tcattgccca
     6061 tcccagcggg aaaatctgat cgccatcggc acgaaggcgg ttaaataatg cgttctctgt
     6121 tacatccagc cagtcagcag cttcagcgta cccccccggc aacgctgcga tagtttttct
     6181 gacagctttc acgtaccact caggctgttt ttctactttc cagtgatgct tacccacggt
     6241 tcacctcctg ttcctgtggt ttaaacccat tctggttttg gctagattga aaacgtgccg
     6301 gataaagaat ctgcatttcg ctgacttcac ccttaaaaaa attggctaaa cgttctgcaa
     6361 gctcgataga tggaatctgc tccagcctct caatacgact caacgtcgct ggattgactt
     6421 gaacacccgc agcaacatgc tgcaaagtga aaccatgcgc cttacgcaca tttcgtaatg
     6481 gtgattgcat atgccctcca aatattgcgc gttatgcatg ttatttcacg caattatttt
     6541 gcgcaagttg atttgcttat cacgcaataa agaaatgtaa taaacgcatg aacataggaa
     6601 accgagtcag acaacttcgc caagcgaaga acatgaaaat cgccgatctc gctgaagcaa
     6661 taggagtaga tgcggcgaac atctcgcgct tagaaacggg taagcaaaaa caatttaccg
     6721 aacaaacact gagtaatatt gccaagagct taggtgttga tattgctgat ctctttacct
     6781 ctgcccacaa aagtaatact gtatataaaa acagtaataa tgaggatgtt gcgcaggtga
     6841 aggatgtgtt ccgtattgaa atgctggata tcagtgccag tgcgggaaat ggccttatcc
     6901 agggcggtga tgtcattgat gtgattcatg ccatcgaata cagaactgat aatgctgtat
     6961 caatgttcgg cggacgacca gccaatcaca tcaaagttat caacgttcgt ggggacagta
     7021 tgtgtccaac cattgagcca ggagatctca tcttcgttga tgtcagcatc aatcagtttg
     7081 atggtgatgg tatatatgtc tttggttttg atgacaaaat atacgttaaa agacttcaaa
     7141 tgattcctga caaactgctg gtgatttctg ataaccagat ttaccgtgaa tggggaatta
     7201 ctagcgaaaa cgaacaccga ttcatggtct ttggaaaggt cttaatcagt cagtcgcaaa
     7261 cccttaagag acataattaa cctcaatatc ccatccatcg gccaccgaaa ggtggctttt
     7321 tattacctat caatttgtat atctcgcaaa tattacttgc atatctcgca atttaatttt
     7381 atcttttgtt ccagaccaac tacaggatta caacaaaatc tggttgcaac acggtgcatg
     7441 tgtcgtaagc agtcagtaaa tgtcaaaaac gaacaggcag gacgcccacg aagtagcctc
     7501 ctgggacata tgaagtccag gatgattcgt tagcaacaaa aaagcgccct acaggacgct
     7561 tagctcttta acaatctggg tatcatccaa ccaatgcaag atttaaggaa tccaaggcga
     7621 attcagatct cgccccaact cacgtaatga tcttggtcgt tcgtacatcg gatttttttc
     7681 cataagaaat ttattttcac agtgaaggca acggcttgta agaaaatgag aagttttacc
     7741 tactggaagc ggatgaagta tcgattttat atttttggaa taacaaagtg ggcacagatg
     7801 cacagttatt tcttttccac tcacaatttc atttttagag taaacaaaag caccagagtc
     7861 aagctgatca aggacatatc cttctacctt ggcacaaaaa tcttcaaact ctgcaatttt
     7921 tgctttgaga tgcatcacct cttcatcacg aaggcggatc gcatcgccaa gagagaagca
     7981 ttctgcctga agcgtgatta gtttgttctg gagttcaatg gttgcagctt taacttctgc
     8041 atccgttttc gcgtcattaa taaccttagc aagaccggca gtctccttta tagcggccat
     8101 agccgcagac agttcagcta tcacgttgaa tactcagcta gttgttgggg atatccagat
     8161 taaccaaatc cttgttgttg gggaataact aggtccacct cgcctgatgt ggctaaaagc
     8221 aggcacataa cagctaagta ttttcaacca gagagaatcc ttagcgttgt ggtgaatgcg
     8281 gctcagcgca cgcgggttaa ggttgaggct gacagtcgac cttctgtgga tacccacccg
     8341 cctggtgtgc aaccttcgcc aggcaccggg aggcacccgg caccacaact ttatgctgtg
     8401 tgtagtcctg gcggtatcag cttgtaccct tgcttccggc tggtaccgtc ctttttacaa
     8461 aacagagaag agcatcaccg gacgacgggc tcataaccca atccatccgg gcggctgcca
     8521 ccgcaggtgt tcttctctgt tttgtggaga aaccaaccga ccttgcaggg tcgatatgat
     8581 ggggagcagc aaaatggcta gcgaacgcag tactgatgtg caggcattta tcggggagct
     8641 ggacggcggc gtatttgaaa ccaaaatcgg cgcagttctc agtgaagtcg cttccggtgt
     8701 gatgaacacg aaaaccaaag gtaaggtctc gctcaacctg gaaatcgaac cgtttgatga
     8761 gaaccgtgta aaaatcaaac acaaactctc atatgttcgc ccgactaacc gcgggaaaat
     8821 ttccgaagaa gacaccaccg aaacgccgat gtatgtcaat cgcggtggtc gcctgactat
     8881 tctgcaggaa gaccagggac aattactgac tcttgccggt gaacctgacg gaaaactccg
     8941 cgcagcaggt cgttaatatc gtttttaata aactgattat ttatctcatc actgaatatc
     9001 tttatatagt gaggacttat tatgtctcag aacttagacg caaccgcaat taatcaaatc
     9061 catgccctta tttctgctca gggtgttaat gaaattatca gtaagattgg tgccgatgct
     9121 gtggcattgc ctgagaattt ccgcattcat gatctggaaa aatttaattt aaatcgcttc
     9181 cgtttccgtg gtgcgctttc cactgccagc atcgatgact ttacccgtta ttctaaagat
     9241 cttgcagatg aaggcacccg ctgctttatc gatgctgata atatgcgagc cgtcagtgtg
     9301 cttaacctgg gtactattga tgaaccaggt cacgcagata acaccgccac tctcaaactg
     9361 aaaaagacag caccgttctc tgccctgttg tctgttaacg gcgagcgtaa ctcccagaag
     9421 tcactggcag aatggattga agactgggcc gactaccttg tgggctttgg tgctaatggt
     9481 gacgccattc aggcaacaaa agcggctgcg gcggtccgta aaatcacgat tgaagcaaac
     9541 cagaccgctg attttgaaga caatgacttc agcggcaaac gctctctgat ggagtctgtc
     9601 gaagcgaaaa ccaaagacat tatgccagta gcatttgagt ttaaatgcgt tccgtttgaa
     9661 ggcctgaaag aacgtccgtt taaattacgc ctcagcatta tcactggtga tcgccctgta
     9721 ctggttctgc gcattattca gctggaggca gtgcaggaag aaatggctaa cgaatttcgt
     9781 gatctgcttg ttgaaaaatt caaagacagc aaagtagaaa cctttattgg tactttcacc
     9841 gcctgatttc attactgcaa atgcccctgc gggggcattt atggaaacgt aattaactca
     9901 ataatcgcct gatggcgagg gttttcttta accaaaattc agcgcggtgc agcgcatata
     9961 cgtggagaac aaaatgtcat ttattaaaac tttttccggg aagcattttt attatgacag
    10021 gataaataaa gacgacatcg atattaacga tatcgcggtt tccctttcaa atatctgtcg
    10081 ctttgccggt catctttcgc acttctacag cgtcgcccaa catgcggttc tttgcagcca
    10141 gctggtgccg caggaatttg cttttgaagc gttaatgcat gatgcaacag aagcgtattg
    10201 ccaggacatt cccgcaccac tgaaacgcct tcttcctgac tataaacaaa tggaagaaaa
    10261 aatagacgcc gtaatccgtg agaaatacgg gttaccccca gttatgagta cgcccgtgaa
    10321 atatgccgat ctcatcatgc tggcaaccga acgccgcgat ctcgggcttg atgatggctc
    10381 tttctggcct gtactggaaa gtatcccggc aacagagatg ttcaacgtga ttccactggc
    10441 accgggccat gcctacggga tgtttatgga acgctttaac gagttatcgg agttacgcaa
    10501 atgcgcatga atgttttcga aatggaaggg tttcttcgtg ggagatgtgt accgcgagat
    10561 ctgaaagtaa atgaaacaga tgctgaatac ctggtgcgta aattcgatgc gcttgaagct
    10621 aaatgtgcag cacaggaaaa caaagtaata ccagtgtcaa ctgaactgcc accagcaaat
    10681 gaaagtgttt tgttattcga tgctaacgga gaaggctggc taattggctg gcgttctctc
    10741 tggtacacct ggggacaaaa agaaaccgga gaatggcagt ggacatttca ggtcggggac
    10801 cttgaaaacg tcaatatcac tcactgggca gtaatgccaa aagcaccgga ggctggagca
    10861 taatgaccac ttttaccgac aaagaactga ttaaagaaat taaagagcgt atcagcagcc
    10921 ttgacgtgcg agacgatatt gagcgccgtg cttatgaaat cgcactccta tctctggaag
    10981 tagaaccaga tgaacgcgaa gcttatgaat tattcatgga aaagcgtttc ggtgacttag
    11041 tagatcgtcg gagagcaaaa aacggcgata acgaatacat ggcatgggat atgactctcg
    11101 gttggatcgt ctggcagcaa cgagctggta tccatttttc aacaatgtca cagcaagagg
    11161 tgaaataatg gagccataca gcctcacact cgatgaggcc tgtcattttc tcaagatatc
    11221 cagaccgact gccattaact ggatacgcac agggcgtctt caggcaacac gcaaagatcc
    11281 cactaagaat aaatctcctt acctcacaac acgacaagcc tgcattgcgg ctcttcagtc
    11341 tccgctgcat actgtccagg tgagcgcggg tgatggcata acagaggaaa gaaaatgtca
    11401 ctcttccgca gaggtgaaat atggtacgcc agtttcacat tgccgaacgg taaaagattt
    11461 aaacagtctc ttggaacaaa ggacaaaagg caggcgacag aactccatga caagctaaag
    11521 gctgaagcat ggcgggtcag caaacttggt gaaatacctg atataacgtt cgaggaagcg
    11581 tgtgtcaggt ggcttgaaga gaaagcacat aaaaaatcac tggacgatga caaaagccgg
    11641 atcggattct ggcttcaaca tttcgcagga atgcaactaa gagacattac tgaatcaaaa
    11701 atttattcag caatgcagaa aatgacgaac cggcgtcatg aggaaaactg gagactcagg
    11761 gcagaagcat gcagaaaaaa agggaaacct gttccagaat acacgccaaa accagcgtcc
    11821 gttgcaacga aggctacgca tctttcattt ataaaggccc tactaagagc cgcagagcgt
    11881 gaatggaaaa tgctggataa ggcaccaatt attaaagtgc ctcaaccaaa gaataaacgg
    11941 atccgctggc tggagcccca tgaagcacaa aggctgattg atgaatgtcc ggagccatta
    12001 aagtctgttg ttgaatttgc actggcaaca ggcttaagac gctcgaacat catcaacctt
    12061 gaatggcaac aaatagacat gcagcgccgg gtggcatgga taaacccgga agagagtaaa
    12121 tcaaaccgcg caattggcgt tgcgctgaat gatactgcat gtcgcgtttt gaaaaaacaa
    12181 atcgggaatc atcaccgttg ggtatttgtg tacaaggaaa gctgtaccaa accagacgga
    12241 acgaaagcgc caacagtaag gaagatgcgg tatgacgcaa acacagcctg gaaagcggcg
    12301 ctgagacggg ctggtattga tgatttcaga tttcacgact tgagacacac ctgggcaagt
    12361 tggctggttc aagccggagt cccgttgtca gtgttacagg aaatgggagg ctgggagtct
    12421 atcgaaatgg ttcgtcgata tgctcacctt gcacctaatc accttaccga acacgcacgg
    12481 caaatagact cgatcctgaa cccatcggtc ccaaattcgt cccagtcaaa aaataaggaa
    12541 ggtactaatg atgtgtaact tattgattta aatggtgccg ataataggag tcgaacctac
    12601 gaccttcgca ttacgaatgc gctgctctac caactgagct atatcggccc tgaaaggaca
    12661 tgttcacgaa cgtgaatcac ggtggacaag gttaaaacta accgggcgat gcgtcaatgg
    12721 ccttgtgaat caaatggcta cttttgcatc acccggtttt atttacgcac gaatggtgta
    12781 atcaccaatg ccgatccact tgtaagtggt cagtgcttcc agccccattg ggccacgcgc
    12841 gtggagtttt tgtgtgctta ccgccacttc cgcacccaga ccaaactggc cgccgtcggt
    12901 aaaacgcgta gaggcgttaa cgtaaacagc ggacgaatcc acttcgttaa caaaacgctg
    12961 ggcgttgcgc atatcgcggg tcaggatcgc atcggagtgt tgtgtgccgt gttcacgaat
    13021 atgggcgatg gcatcgtcaa gatcgctgac gattttgacg ttcaaatcta atgacagaaa
    13081 ctcatcgtca tactcttcgg ctttaacagc aaccaccttc gcagggcctg cctgcaactg
    13141 cgccagtgca gctgcatctg cgtgtaatgt cacgccgctt tccgccattt gtttgcttaa
    13201 tgcgagcagg aagctatcgg cgatgttttt attcaccagc aacgtttcaa ccgtattaca
    13261 tgtgctcgga cgctgagttt tcgcgttgac gatcactttt aatgcttcag cgatctctac
    13321 actttcatca acgtaaatat ggcatacgcc tataccacct gtgatcaccg ggattgtcga
    13381 ctgttcacgg cacagtttat gcaaaccagc gccaccacgc gggatcagca tgtcgatgta
    13441 tttatccata cgcagcattt cactgaccag cgcacggtca ggattatcaa tcgcctgcac
    13501 ggcacccgcc ggtaagccgc aggatttcag ggcgtcctga atcaccgcca ccgttgcagc
    13561 gttagtgcga cacgtttctt tgccaccgcg caggatcacc gcattaccgg ttttcaggca
    13621 cagcgaagcg acatcaaccg tcacgttcgg gcgcgcttca taaatcacgc caataacccc
    13681 cagcggtacg cgacgacgct caagacgcag gccgctgtcc agtacgccgc catcgattac
    13741 ctgccccacc ggatcggcga ggttacacac ctggcgcaca tcgtcggcaa tgcctttcag
    13801 ccgtgcgggc gtcagtgcca gacggtcaag catcgcttcg ctaaggccgt tggcacgcgc
    13861 gtcagcaaca tcctgcgcgt tagcgttgag gatgatttcg ctttgtgctt ccagttcatc
    13921 ggctattttt tccagtacgc gatttttttc gcggctggag agttgcgcta atttatacga
    13981 ggcttgcttc gcggcaatgc ccatttgttc cagcatcagc ctgctcctta acgggtaatc
    14041 atgtcatcac ggtgaacggc aaccgggccg tattcatatc ccagtattgc atcaatttct
    14101 tgcgagtggt gtccggcaat acggcgtaat gcatcgctgt tgtaacgact gacaccgtgg
    14161 gcgatatcgc ggccttcgag gttgcaaatg cggatgactt caccacgcga gaagttgcca
    14221 gtcacgcttt taatgccttt cggcaacagg gagctgccgc gttccagaat ggcggcagtt
    14281 gccccttcat ctaccgtgat ttcccccgca ggcggcgcac cgaaaatcca gcgtttacgg
    14341 ttttcaagcg gagtcgcctg ggcatggaac agcgtaccga cggaaatgcc ttccatcaca
    14401 tcaccaataa cgcccggctt gctgcccgcg gcaataatgg tgtcgatacc cgcacggcaa
    14461 gccacgtcag cggcctgcaa tttggtactc atgccgccag ttccgaggcc tgaaacgctg
    14521 tcaccggcaa tcgcgcgcag tgcgtcatca atgccgtaaa catctttaat cagttctgcc
    14581 tgcggattgc tgcgcggatc agcggtatac aaaccttttt gatcggtcag cagcaacagt
    14641 ttatcggcac ccgccagaat cgccgccagc gcagaaaggt tatcgttatc gccgacctta
    14701 atctctgccg tagcgacagc atcgttctca ttgattaccg gaacgatatt gttatcgagc
    14761 aacgcacgca gggtgtcgcg ggcgttcagg aagcgttctc ggtcttccat atcagcacgg
    14821 gtcagcagca tttgcccgac gtgaatgcca taaatcgaaa acagctgttc ccacagttga
    14881 atcagtcgac tctgccctac cgccgccagc agttgtttcg aggcgatagt cgctggcagt
    14941 tccgggtaac ccaggtgctc acgtccggcg gcgatcgcgc ccgacgtcac aataacaatc
    15001 cgatgcccgg cggcatgtaa ctgcgcgcac tggcgaacaa gttcaacgat atgggcacgg
    15061 ttcagacggc gcgatccgcc tgttagcaca ctggtgccga gttttaccac cagcgtctgg
    15121 ctgtcactca tgattctctg ccattcaatt ttaggaaaaa tgatatcaaa cgaacgtttt
    15181 agcaggactg tcgtcggttg ccaaccatct gcgagcaaag catggcgttt tgttgcgcgg
    15241 gatcagcaag cctagcggca gttgtttacg cttttattac agatttacta aattaccaca
    15301 ttttaagaat attattaatc tgtaatatat ctttaacaat ctcaggttaa aaactttcct
    15361 gttttcaacg ggactctccc gctgaatatt cgcgcgttaa ttaaaatcag gaatgaaaat
    15421 gaaaaagagc actctggcat tagtggtgat gggcattgtg gcatctgcat ccgtacaggc
    15481 cgcagaaata tataacaaag acggtaataa actggatgtc tatggcaaag ttaaagccat
    15541 gcattatatg agtgataacg acagtaaaga tggcgaccag agttatatcc gttttggttt
    15601 taaaggcgaa acacaaatta acgatcaact gactggttat ggtcgttggg aagcagagtt
    15661 tgccggtaat aaagcagaga gtgatactgc acagcaaaaa acgcgtctcg cttttgccgg
    15721 gttgaaatat aaagatttgg gttctttcga ttatggtcgt aacctgggcg cgttgtatga
    15781 cgtggaagcc tggaccgata tgttcccgga atttggtggc gactcctcgg cgcagaccga
    15841 caactttatg accaaacgcg ccagcggtct ggcgacgtat cggaacaccg acttcttcgg
    15901 cgttatcgat ggcctgaact taaccctgca atatcaaggg aaaaacgaaa accgcgacgt
    15961 taaaaagcaa aacggcgatg gcttcggcac gtcattgaca tatgactttg gcggcagcga
    16021 tttcgccatt agtggggcct ataccaactc agatcgcacc aacgagcaga acctgcaaag
    16081 ccgtggcaca ggcaagcgtg cagaagcatg ggctacaggt ctgaaatacg atgccaataa
    16141 tatttatctg gcaacttttt attctgaaac acgcaaaatg acgccaataa ctggcggctt
    16201 tgccaataag acacagaact ttgaagcggt cgctcaatac cagtttgact ttggtctgcg
    16261 tccatcgctg ggttatgtct tatcgaaagg gaaagatatt gaaggtatcg gtgatgaaga
    16321 tctggtcaat tatatcgatg tcggggctac gtattatttc aacaaaaata tgtcagcgtt
    16381 tgttgattat aaaatcaacc aactggatag cgataacaaa ttgaatatta ataatgatga
    16441 tattgtcgcg gttggcatga cctatcagtt ttaatggata ttgccggatg tgatacatcc
    16501 ggcacatttc actcacgccg ttaacttcac cggttcatca cgaaaatcat ccgccggttc
    16561 cagcttcaga ttcagcgtcg tcagcagttc acgcagcttc tcgtgaaact cacgcagggt
    16621 gtgctccagt cgttcaacca cttcagtgtc ttttaccgga acactcttcc agtcgcctgc
    16681 tttatcgaac agaccaaact ggtaactgta ggtaaaacgg gattcctgcg cttcaagctc
    16741 catccaccag ccccagaact cacgcacttc cggtgccggt ttcacgttga cgcatacagc
    16801 cagacaatcg aaaaagaatc gattatcttc gcacttacct tcacgaatat acgggcctag
    16861 tgcggtaaat tttttgatca atctgctctt cgggtgtcca ctcggtaacg tcattgctat
    16921 ctcctgttgt gatgcaactg ttttaccaaa ttggcaaaat ttagcaaatt tttaacacaa
    16981 acgtttttcg atccagtctg tgatttcctg aagacctttg tcaaaattcc gatacaccgg
    17041 gttaaatggg atctctaata atttaccgtc agcagatgat gaggtgatta agcgtgagtc
    17101 ctcttccggg ctgaacggat cgttcttcca gtagcctgat aacattggcg ttgggcagcg
    17161 acgtcccagc aatccttgca cttttaatga atagcgattc agctccacgc gcaacgcttc
    17221 atcggaagca tcatgcatcc ccaaacgact ggccagaacg tcaagataca tttccggcac
    17281 ctgttgctgg cacttaaaat cactcaacag ggtatgaact accggaccaa gacaggcaac
    17341 cgctttcaga cgcggcgatt caagatatgc cagacgcacg gcaacgttag cgccgaaacg
    17401 gaaaccaaag gccgcgacgc gagtgtgatc cacccacggt acgttaggca gcgcctttaa
    17461 gacgtgctga tgcaacaggc tggagtcctg ggtgagcttc cattttgaag aaaagcccac
    17521 cgatggcata tcaatagtca gcatcgcaat gccgcgcggc gcaaaataac gttcatacag
    17581 gctgtaatag tccgtctgca tcgcatccag accaccacac attaatactg tcgggaacgg
    17641 gccatcgcct ttcggcatat gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa
    17701 ctccatctgc cgcatcgtgc ccggcagacg ctgggcggcc tcttcatagg cgcggtttga
    17761 taaagcctgc gcttgctcgg ccaggtcatc tcctttcaga tgaggatagg cggcaatgtt
    17821 gtacaacgta gccgcatgca gccagtgtcg accactgagt tgcggatctt cttccgcaca
    17881 ggctttttgt tgccacacca tcgcctgggt ggcccactca taaatccagt tgccaccacg
    17941 gtagccaatc acagtatcgt ataaatcatc gtcggtacgt tcagcatcgc tcatcacaat
    18001 acgtgcctgg acgtcgagga tttcacgtgg gtcaataccg cgccagatcc acatcagacg
    18061 gttaatcatg cgataccagt gagggatggt tttaccatca agagccgact gcacaggcgg
    18121 ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg
    18181 tttgaacagg gtttcgctca ggtttgcctg tgtcatggat gcagcctcca gaatacttac
    18241 tggaaactat tgtaacctgc ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt
    18301 gaaaagatta gcgaccggag attggcggga cgaatacgac gcccatatcc cacggctgtt
    18361 cgatccaggt atcttgcggg atatcaacaa catagtcatc aaccagcgga cgaccagccg
    18421 gttttgcgaa gatggtgaca aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag
    18481 taccaccggt atccaccagg tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc
    18541 gtttcagcac tttaagctcg cgctggttgt cgtgatcgta gctggaaata caaacggtat
    18601 cgacatgacg aatacccagt tcacgcgcca gtaacgcacc cggtaccaga ccgccacggc
    18661 ttacggcaat aatgcctttc cattgttcag aaggcatcag tcggcttgcg agtttacgtg
    18721 catggatctg caacatgtcc caggtgacga tgtatttttc gctcatgtga agtgtcccag
    18781 cctgtttatc tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg agattataga
    18841 gatctggcgc actaaaaacc agtatttcac atgagtccgc gtctttttac gcactgcctc
    18901 tccctgacgc gggataaagt ggtattctca aacatatctc gcaagcctgt cttgtgttga
    18961 caacattttc tgctaaccct gtgacctgca atactgtttt gcgggtgatc gacaaggaga
    19021 cttaacgtgt ctgaactgtc tcaattatct ccacagccgc tgtgggatat ttttgccaaa
    19081 atctgttcta ttcctcaccc gtcctatcat gaagagcaac tcgctgaata cattgttggt
    19141 tgggcaaaag agaaaggttt ccatgtcgaa cgcgatcagg taggtaatat cctgattcgt
    19201 aaacctgcca ccgcaggtat ggaaaatcgt aaaccggtcg tcttgcaggc ccacctcgat
    19261 atggtgccgc agaaaaataa cgacaccgtg catgacttca cgaaagatcc tatccagcct
    19321 tatattgatg gcgaatgggt taaagcgcgc ggcaccacgc tgggtgcaga taacggcatt
    19381 ggtatggcgt ctgcgctggc ggttctggct gacgaaaacg tggttcacgg cccgctggaa
    19441 gtgctgctga ccatgaccga agaagccggt atggacggtg cgttcggctt acagagcaac
    19501 tggttgcagg ctgatattct gattaacacc gactccgaag aagaaggtga aatctacatg
    19561 ggttgcgcgg ggggtatcga cttcacctcc aacctgcatt tggatcgtga agcggttcca
    19621 gctggctttg aaaccttcaa gttaacctta aaaggtctaa aaggcggtca ctccggcggt
    19681 gaaatccacg ttggcctggg taatgccaac aaactgctgg tgcgcttcct ggcgggtcat
    19741 gcggaagagc tggacctgcg ccttatcgat ttcaacggtg gcacactgcg taacgccatc
    19801 ccgcgtgaag cctttgcgac cattgctgtc gcagctgata aagtcgacgc cctgaaatct
    19861 ctggtgaata cctatcagga gatcctgaaa aacgagctgg cagaaaaaga gaaaaatctg
    19921 gccttgttgc tggactctgt agcgaacgat aaagctgccc tgattgcgaa atctcgcgat
    19981 acctttattc gtctgctgaa cgccaccccg aacggtgtga tccgcaactc cgacgtggca
    20041 aaaggtgtgg tcgaaacctc cctgaacgtc ggtgtggtga ccatgactga caataacgta
    20101 gaaattcact gtctgatccg ttctctgatc gacagcggta aagactacgt ggtgagcatg
    20161 ctggattcgc tgggtaaact ggctggcgcg aaaaccgaag cgaaaggcgc atatcctggc
    20221 tggcagccgg acgctaattc tccggtgatg catctggtac gtgaaaccta tcagcgcctg
    20281 ttcaacaaga cgccgaacat ccagattatc cacgcgggcc tggaatgtgg tctgttcaaa
    20341 aaaccgtatc cggaaatgga catggtttct atcgggccaa caatcaccgg tccacactct
    20401 ccggatgagc aagttcacat cgaaagcgtt ggtcattact ggacactgct gactgaactg
    20461 ctgaaagaaa ttccggcgaa gtaattattt gatttgctgc cggatggcgt ttaatcgcct
    20521 tccggcagtt tcatccttca ttatccttcg ataaaagcca tccctgtaaa tgtccgtcgc
    20581 gggttgccac gttcaatctg gtgatggaac attcgccgct gcgatttcag cgccgcgcta
    20641 ttttcctgtt gctgctgctc cagcttccag gcaatcagca atcgcgccag ccgtttgtta
    20701 gcatgctggc tgcgctctga ctgaaccttc acgctaatac cggatgccag atgcgtggcg
    20761 cgtaccgccg agtcggtttt attgacatgt tgaccgcccg gccccgacga acgcagcgtc
    20821 tcataacgga ttgcatccga ttgttccttc tcatcagcgg taaaacgccc aatgcccaga
    20881 aaccagtttt tgcgcccatg atgaggccga tacggactcg gacaaatcca ctgaatagtg
    20941 ccacaccagc tttcgcttaa tgcccatgcg ttatcgccat ccagagaaac cagcgccgaa
    21001 cgcagcgtgt cagagtagcg gcccgtttct gtttccagca ccgttacagc gacgtcttgt
    21061 cgggtaaaaa cgaccaacag agaagaacgc tgcgccaatc gggtagccgc gtccggggtg
    21121 taaatctggc atccccacta cgcgctgcat gttgggtaaa tttgccgtgg tgtgtaattg
    21181 ttggatcgct aaactctcga tccacaggtc atcagatgcg atggtaaata ccgcatcgga
    21241 taagggacga atatatttgc ccatgtacca ttccatttat actcgtcata cttcaagttg
    21301 catgtactgc gactcgaatt atttagagta tataaatata aaaatcagga aatggcagac
    21361 gaatccaggg caagaaaaaa agaattaatc ctggaaaggt ctgcaaagag tcattgataa
    21421 aggatttgtc atgatttacc tccttgcagt ttgtggaata agaaaacgcg cacataccag
    21481 gcgggcgtta ttttcattgc aagctggatt taatattgcg gtttatatcg catataaaaa
    21541 ttagtaaacc acgttccccg gcattcaacg cgctgctgct taactgtctg aaaaccataa
    21601 cgttcaaaaa agggttttgc ggttatgctg gcgtccaccg taagttcgga ttcagactta
    21661 atcaaaggtt ttaacaaagc gcaggcaacc ccgcggcggg tgtattcagg gtcaacaaat
    21721 aacatatcga tataacgttc aacgcaggta ataaaaccaa ccggttgtgc attaatgacg
    21781 gcaacccgca cttgtgattt cgcgagtttc tccttccagc gagattcgtc aatctgcgcc
    21841 caggcggcaa tttgttgtgg tgaataatgc tgactggaga tcatcgtaac cgctctaatg
    21901 aaaatagcgc atagttgctg aaaatcgcca ggctgatagt ctcttatttg tatgttattc
    21961 ataatataaa ttcaaaaacg catgcgaaac gcttctgcca ttttcccaat tttatgcatt
    22021 tggttgttat ctcgagcctg tttatgaggt tcaggtttca gaatggcaat gagcaaccat
    22081 gcttgctcat caaacgcacc ctgacaatat accaaatgcg cttcgtcatt cgttctgctg
    22141 aattggcgca actgtggcgg aaatggatta ttcacatttg ccagatgaat atgagcaact
    22201 cgctcaaatt tgattaatgg ccaggtaaac gagtcgtcgt agagtgcatc gcgaccaaat
    22261 atatctggca aaacaccgtc acgcttatag gaaataaaat ccgccgttaa cgcatcaagt
    22321 tcctctgctg taagttgcag gcgaataagt tttgttttga atacccgcat ccttattcct
    22381 taaagtcatt gaaatcatca tccgtcatat cattaagata tttttcagct cggcgtgtaa
    22441 tttcctctaa tcttgcagca cttggacgga agcgcgcctt tatgggcttt ttctcctctt
    22501 gttcagcaag catgtccatt aacgcttcga atgcgctggc acttaagaga tatcctgcgg
    22561 ggcgattatt agaaagaacc gcaaccggtt gatcaataaa gtatttagct gggtttttac
    22621 gtaactcagt aatattgacc gatttttcag cgagaattcg atgcatgcag tgatcccctc
    22681 ataataatgc acaccagaat agacataata gtatacatca taatcccagc accagttgtc
    22741 tttccatttg cggatcaagc aacgtcacat gcagccccac cagtcgcaca ccgcgcccgc
    22801 cgcggcgttc atcccaggtt ttacgcgcgg tggcgattaa gtcagcttta ttcagccgcg
    22861 gccagacgtg ctcctgagtg gtttgctgaa aatcgtcgaa ctttaatttc accccctggc
    22921 gggcaatcag taaatcaggt tttacctttg ccagacggcg ttcaagttcc ggatacagcc
    22981 gctcgataat cgcttcacat tcagaccagt ggtggatatc ttccgccatc gtgcgttcca
    23041 cgccgacgga ttttcgcaac cgttcgctgt taacgtcgcg ctcgtcaatc ccctgactac
    23101 gctcccacaa aatgcggcca aatttgccaa agcgtttaag cagcatcacc agatcacact
    23161 tttgtacatc accgcaggtt cgtagcccca tcgcttccag ttttgccgcc gagactttgc
    23221 cgacgccggg gatttttgcc agtggtaagg tttgtaaaaa tgccggaact tctgccggcg
    23281 taatcacaaa ctggccgttg ggtttattca tgtcggaggc gattttggcg agaaacttta
    23341 cgggtgccac gcccgcagac gccgtcagtt gcagctcgtt gaaaatcgtc tggcggattt
    23401 cctgggcgat gagggtcgca gaaccgtggc aatggacgct atcggtgacg tcgagataag
    23461 cctcatccag tgacaacggt tcaatacgcg aggtgtagcg cgagaagatt tcgcggatat
    23521 gatttgaggc ttctttgtag gcgtcaaagc gccccggaag caaggtgaga tgcgggcata
    23581 atttgagcgc catccctgtc ggcatagcgc tacgtacgcc aaatttacgc gcgggataat
    23641 tggcggtgct gatcaccccc cgacgttcgc ggctgccgcc aatagcaata gggatatcgc
    23701 gcagggcggg attgtcgcgc atctccactg cggcgaaaaa gcagtccata tccacatgaa
    23761 tgattttacg cattactcac ctctcaacac tggtaaagta tacagtgatt tcagggtttg
    23821 agaaatgcgt aaagattcag catttagcgc gttcgctgcg aaagcacctg ctgcttatcc
    23881 agcttttgca ccagcggctg caccacttta tccccatgct gaccaaagta ttgatacagc
    23941 gtatcggacg cacttttggt cagcaccata atctcgatgc gccgattacc cgcactttgc
    24001 ggattcttcg cgtccagcag catctggtcc gccattgcgc ttacctgcat cactttatct
    24061 tccggcattc cggcctcttc cagcacccga cgagccgaaa gcgcgcggtc acccgaaagg
    24121 ttccagttgt tgtagatatt gtttttgtag gccatcgcat cggtatgccc ggtaataata
    24181 attttattat cgagcgagtc gaacactggc gcgagctcca ccagcagcgt tttaaagaac
    24241 ggcataatct tcgcgctgcc gcgttcaaac atattgcggt tctggtcatc tttaatcagc
    24301 acgcgcaatc cctggggaac aatctccatc tccagattcg cttccatatg cgcatcacgg
    24361 gcaatggtgt tgatactggt tgccagctcg ccaagctcgg tggcggattt cttcttcagc
    24421 agcgctgttt tctcgttgac gtctcgcgct tttttctccg tctcttccgg caccgccacg
    24481 gtggcaggtt ttggcggatg agacgggctg attttattta acggcgacaa tccgccgcca
    24541 ctgaaaatcg actgcccgtg cagcgcggcg ataatgcttt cacgctcgga tttactgacg
    24601 ctgttgacaa tccacagcgt cataaacagc gccatcattg ccaaagtaaa gtcggcgaac
    24661 gccaccttcc acgccccgcc gtggtgcccg gcatgattct ttttgatctg ccgccggacg
    24721 atggtggttt ttgccccacg atcgcggcgg ttacccgtct ttctcatgct tattcttgct
    24781 ccagcatcgc attcacccac gcgtcgaggt tggcgaaggt cggtttggac gccagatgca
    24841 gtagcttacg cccggcgtcc accgccagca gcgtcggttt gccgcctgcc tgcgccacca
    24901 gcacggtacg cacgcattcc agcaaagaat gttctgcccg cgcctgttgt tccatggcgt
    24961 tggcgagtgg gtccatcagg cagtagcaga taaagacgcc taaaaatgtc cccaccagcg
    25021 ctgccgccac tttcaggcca atcagggcga tagagccgtc aatcgactgc atggtgatga
    25081 tgatgcccaa caccgccgca caaatgccaa accccggcat cgcttcggcg gtacgttgca
    25141 gcgagcgcga cggcgttagc agcgactctt ccgcggtgtc cagttcctga tcgagaatgc
    25201 cttccagttc atgggcgtcg attttaccca tcgccatcag gcgaaagtta tcggcaataa
    25261 aggtcaccag ccgcttctgg gttaacacca gcgggtattt ctggaaaatg gtgctctctt
    25321 ccggttgctc aatatgttga tccagcatgc gcagaccacc gttttgcacc atttcgagta
    25381 actcatacag gcacattaat agctggcgct gaaactccgg cccgagctgt tttttactga
    25441 ttacgccttt aatttgatgg gcaatctctt tcagcacatg tttcgggtta ccaataatca
    25501 tcgccccgaa accggcaccc aaaataatga tcatttccgc cggttgccag atagccacta
    25561 attgcccgcc cgcttcaaaa taaccgccaa atacgcagcc gagcaccacc agtaaaccta
    25621 atattttttg catgatttct tcactttaat tcggatagac aaaatcacga attttctgcg
    25681 cgatctgttt attcagttga caaattcggg cctcggttag ccctaatacc agcgcaatct
    25741 ctttgagatt catctcatgc tggtaataca tggagaggat cagttgctct ttttcgctta
    25801 attgtgaaag cgcctgttgc agcatttgct gggtgacaaa ctgatcttcc agctccctgc
    25861 cagcaagcag aatgccaggc ccttcgccgt tcagcagttc gtccaggctg gcgagcgttt
    25921 ccgccccttc cagttgctga tattcgagat aatcttcatg cgagatccct tccacggcca
    25981 gttctgacca ctccggttcg tgccccagtt ttttgcgcgt ttcgcgaatc agatctttca
    26041 tctgatgata tttctgtcgc agctggcgcg gtcgccagtc gagagcgcgc agctcatcca
    26101 ggatcgcgcc gcgaatacgg tgcacggcat agcccgcaaa accttcgtcc ggcagcccgt
    26161 agcgacggat ggcattaagc agccccatta acgccgtttg ttccatatcc tgacgatcaa
    26221 taatgcaatt acactgcggg gcaagttgcc gcaccacttt acgcaccaac ggtaaatagg
    26281 cctgcaagta gtggctctcc tgctgcgggg ttaataccgg cgcggcagca aattcttccg
    26341 actcgaatac atcctgtagc atatttatat ccgcctggtg attactggaa gaccactttt
    26401 ttcaccagca aatccttata cggtgcgctc attttgcgac gctgaagatc tttctttaac
    26461 gcgtcagaaa tgagtttgcg gatggcggat attttcatgc cacgcaaatc ttcaaatttc
    26521 atttccgtca ggcattcaac cgcaatactt tgatacagcg gctcctgatg ttttattttt
    26581 tccgcttctt tgtcactggc gaccaccatc actaattccg ccgacaaata atggtccgcg
    26641 ccgttattgt catgcagcgt gacaatggtt tccggcaacg tcacaaacac gcttttgctc
    26701 tcatccaggg tttccaccgt ttccgtctgc gcggcggcag tcggttttcc ttttccggca
    26761 taatgatgcc agacacccca tccagctccg gcccccacca ccaacgccag aacggcgctg
    26821 accacgcccg ccaccacgat tttcttcata ttgcctttac actttgatca aaacggattc
    26881 gttctgttga aattgaggcg catcatcaag ctgtaaggca gcactcacct cttcctgctg
    26941 atggcccgac tgttgttgtt gctgcgactg cccttcggag gaaacctgta cgttcacctc
    27001 cataaaattt tgcgccgtca gatgctggcg gagatcgtcg ctaaactgct gtaacgcgcg
    27061 gcaaacttca ctttggttcg cgccgatgtg caccatcagt ttgccgttgt cgagttgtac
    27121 ggcgatctcg agcttgccaa gcgacggtgg atcgagacgg atggtggaga tctgctgttg
    27181 tttgttgagc tgaaagtgga tctgatcttt caacagcgtc gtcagttttt cgcccagttc
    27241 gtccatcgaa agcgtgacgg gtttggcgac aatctcctga tgatcgctga ccagctccgg
    27301 cgtcttaacc tgcaacaccg cacgcgcaat aaccggttcg ctgtccggca ctttttccgt
    27361 tttacgctcg acctgcgggc gcgcggtcac acgggttagt tcaggtttcg ggcgagctgg
    27421 ctgtgttgag acgcgcggct gcgtcgacgc aatggcatgt aaatcttcac tggcgtaagt
    27481 cgccagtctg gcctgctgtt cgggtttctc ctgcggcaac tgactgatca gttcctgcaa
    27541 ctgcggcggc aacggcgtga gttcctgctg ttgcgtcggg cgttgcggcg cgttttgcac
    27601 caccgctttc gtcaattgct ggataaccgg cgatgttgcg acgtttcgcg gctgcggcgt
    27661 gtcctgatgt ggcgcggctg ggtgcggaag taacagcgcc attagcgcct gcatcgccac
    27721 cggatcgttc tcctgcgggt gcgtcgcctg ttgctgactg gttttagcgc tatgaacgcg
    27781 ctccgccacc gccgcgaccg ccattttcgg cagggtaaac gcgggcgcgt tttcaccgct
    27841 caccggcggt aaaatatccg ctttcagcga cgccgtttcc gcaagcgttc cgagcgtggc
    27901 aagcaatgcc ggattcatga cttctccgaa agtaaattaa caattgagta ggccagtacg
    27961 ccttcctgat gccgctgatg ctgttccata cgcgctttca acacttctgc gcgctgctcg
    28021 cgccgtttaa tcagggcgtt aaacgcgtca tacagtcgcg ctttcactgc atccaactgc
    28081 gccgcatcgg tttccgccgc cgcgtaatcg tgaaactggc gtatcaactg ctgatgcagt
    28141 gccggaaggc gtcgccagcg ttgcttgtcg agggcttcat tcatcgcctc gaccagtgcg
    28201 aaaagtttcc tgcgttgttt ttccatcgtc ttaacccagc ttgcccgaaa gcccttccca
    28261 gccttcgcgc aggttgctta agatcagcat cacttcatcg attttctctg ccgacagttc
    28321 gccgctggcc tcatacaatc ggtaaacgca gtggtcatag agtcgcgaaa ggttgaccac
    28381 cagctcgccg ccagtttcaa actccagcga actggtgagc gcattgagaa tgtcgatgca
    28441 tttattgatg ctctgggctt tcttctcgaa acgccgccct tcaatatggc ttttggcgcg
    28501 ttccagttcg tccatcaggc cagaaaacag caccagcacc agttccagag gcgatgccgc
    28561 cgcagtacgc gccgcgagat cgatctcttt atattgcgaa tagccgtcct gtacttggta
    28621 catctttagg gatattcctt ctgagttacg cgaatgccgc catgctggct ttcatggtgt
    28681 acacctcaac cagcgtgttg gtgtactcct ccagataacg gtcgtagttg gcgttatagg
    28741 tatcagtaag ctgatcgcct tcgtcctgaa ttttgctcat ctgatcgtca atgttctgct
    28801 ggcgcagggt gatgatgccg ttgctggagt ccgtgtaggt gttaatcagg tcgtccatct
    28861 gcgccaccat gctgttgtcg ccaacgaaaa tagaggtcag accgtcagga tttttcgcca
    28921 tttcgtcgtt gaactggtcg gagtcaatct gtaagtggcc gtgagaatcg agggtgatgc
    28981 catagtcaac aatcgacacg ccgttgtagc tggcatgggc gatgtcatcg agctggttcg
    29041 ccagtgaact gagcccggcg tcgcctgcaa ataccccggc gctggtactg tcatcaccgt
    29101 gagtggtcag cgaatcaacc gtatcaatca aggtgttata ggcatcgaca aaggtctgta
    29161 ctttctcctg gctggcgctg gaatcttcgc tgatgttaaa ggtggtgaga tcgctgtcag
    29221 aatcgctgac ttcggtaaaa gtcatggtga cgccggggat cacatcatca aatgtattgc
    29281 tgctgttggt gatcgcaggc cctgtggcgc taccgagatg aataatcgcg tcctgagcgg
    29341 aggaaacatc cgtggcaacg ggcgcgctgg tgctatcgtt gctggcatca tgccccgtta
    29401 cgctcaccga aaacgcgctc tgcgccccgg tgctatcgga ggtgagcata atcgtggtgg
    29461 tgccgtcggt ttttaccagt gccgccgaca cgcctggatt gtcatcggag tcgttaatcg
    29521 cattcaccag ctcgctggcg tcgataaaac catcgccgtc accgttctga tctgccgccg
    29581 acagatcgat atccatggtg ctgtcgccca tcgtcagttc gaaggtgccg gtggcggaaa
    29641 aggcgtcgtc gcccatactg aatgtggtct gctgcccctg cgccagttgc tcaacgaaaa
    29701 atgagtagct tcccgcctgc gcctgggaat tggcggaaac ggtcgccgag tcattattgc
    29761 tggtggcggc aaaagtcacc gggccgtcgg tatcgctgtt cagcgcgtca acggcgctct
    29821 ggaaatcgct caacgctgag ctgagcgagt ccaggccgct gctttcagca tccagctcgc
    29881 tctgcttctc ctgcaaattg gctgcctgag tggcaacatc cgcataagcg atttcttgcg
    29941 caatggttct tgggttgatc attgatgact ccggataacg atgtcagatt attggcaaaa
    30001 ggcgtgccaa tattttttat gtgaataatc aatagattga aaaacaggcg gaaatggcgt
    30061 ttccggtctg gcggaaaaaa gcctgccgca gaaagcacaa caccgcccgc aggcggtgtt
    30121 gctcgtaggc cgaataaggc gtttacgccg catccggcta tcggtgcaca gtgcctgatg
    30181 caacgctgac gcgtcttatc agcaagtccg ccattactgc agcaggctgg aaaccatgct
    30241 ggacatgctg ttggactgtt tcagcatggt gatgccggtc tgcatcagca cctgctgttt
    30301 ggtcatgttg gacgcttccg tcgcgtagtc ggtatccatg atgttaccga tagcaacttc
    30361 ggtgttgtcc tgcatgctgg tcaggttgtt ggcagtgtcg ttcagacggt taatagacgc
    30421 acccagcttg gactgaatct gagaaacgtc atccatcgcg gtagaaatgc tggtaatcat
    30481 ctggttagcc gagccagacg cagtcagctc agtaccgcca gacacgcctg cagtacccgt
    30541 ggtgtctgcc tgatccgcac tgaaggagtt gctgatagca gaaagatccg tcaccagggt
    30601 attcagttgg cttgaaatgt tcacggtcat ggtgtcggaa gattccgcgc ccacctggaa
    30661 ggttaccgcg ctctggaaca ggccgtcagt accggtgttg ctggtgccgg aaacaccgaa
    30721 caggttggtg ccgccgtagg tggtgttttg cagcatgtca gacatctgct gacccagttc
    30781 gtcgtattcg tcctgcatcg cctgcaggtc gtcgtcgctg taggtgccgt tcgctgcctg
    30841 agttgacagg tctttcatac gacccagcac gtcagacatt tcatcgaaca tgctgtcggc
    30901 ggtttgcagc atcgcggtgg catcattgat attgctcagc gccacgccca taccgcttga
    30961 ctgtgcagtc agacggttag caatttgttt gcccgctgcg tcatcggcag aagagttaat
    31021 ccggttacct gtcgccaggc gttccataga tgtagacaga gaagagctgc ttttgctgat
    31081 tgcattaacg gcggccattg aggcgttatt ggtgttaata gataacatga tactgctcct
    31141 tgggattaat cgttttcgtt tcgataccct gtaaaaacga cacctcagga gcaaacttaa
    31201 aatcgccgta aaaaaaattt cactgcggtt tatctgatgc cacttttagc taaaaaaagc
    31261 agagtttaaa ttcagataaa aagattatta gcaatatctg ccacatggcc aggtatcaaa
    31321 atatgtcttt atcaattgct tgatatagtt aagattatca tcaattaccg cgttttcttc
    31381 tcaatgtact gatgccaaaa tttacattgc gttgcaaata gtacacattt ggctattgta
    31441 cctgtcctgt catttcgtta catttccacg ccatatagtg attaagaata atcctattac
    31501 cataaaatat tagttatcac gcagaaaaat catcttctca gggacgtata agactgtgct
    31561 ttatgcaaag cactatcagg aaacatttat cccttgctaa ggtaaactca actatgagta
    31621 ttattatcaa caactggcgg atggacccgt cgcttaatgc cttaatacat tgcgaaacgg
    31681 gtgaaacacg tcgtctcggc gaataccatt ttattttgct ggaaacatta gcaaaaaatg
    31741 ccgatgtggt tttatcgcga tcttatttat gtgccgaagt atggaagaac cgcattgtcg
    31801 gcggaaacag cctgccgacg gcgatccatg cgttacgcgt tgctatcgac gatgatggca
    31861 aacaacagaa cattattaaa accatcccga aaaaaggtta cctgtgcaat aaagagtatg
    31921 tgtctttgcc ggagtcatcg ccagcggaag cgctgattat taccaaccag atacaggaaa
    31981 ctgtgccagg agagatatct tcgacaacac cgcctgtgcc tgttagaaaa aaacataaag
    32041 gactgatggg gttagccttg accgcggctg ttatatttat tggctcgacc gtggggtatt
    32101 cacacttgaa aagtacacct gatgccccgc agctggtaaa agagtcaatt aacagtccaa
    32161 gaataaaaat atttcacctt agttctggaa aagaaaataa ttccgtaccg ttactgtcac
    32221 aaaccctcgc accagggaaa gacaaactcg ataatttatt gtcatcgcat aatatgacaa
    32281 tgacaaccta ttataaatat gttcgtaacc gactggaaag tgatattgtt ctgcgcaacc
    32341 agtgcaatgg tagctggcaa ttaacgttta atgtggaagg ctggcagaac agcgatatta
    32401 atagcgcgat gtatcagaat ttagagaagt tgttaaatac tgtgcagaag tgctgatgag
    32461 tgccaatata tgccggatac gacgctaaag gcgtcttatc cggctgataa ttaccccaat
    32521 aaattcctca ccgtctggct ggaaattcgc gcctgaccgt tcaccgcctg caataacacc
    32581 tgataattat gattcgcgct atcgagcacg ccgccgaggt tttccgatgc ctgcacggcg
    32641 ttttccgttt gcgggaagcg ggacatgccg tcgatcaact ggcgggcttt ttcctgttgt
    32701 accgccatct gcgcgcgctg ctgattaata ctttctaacg tctgagaaat tcccgccccg
    32761 ccctgttgca ggctttgcgc taagcggtcc gcctgtgacg gttcggcaaa gattttcagc
    32821 ggcataaacg ctgatttatc accatcgctg cgcacgctta aagtgcgctc gatgttaggc
    32881 cactgttttt cggtggtgga gaaactgatg ccatccgctt gctgatgcag ctgcacgccc
    32941 acgcggcgca gggcgttggt cagccgcgtc tgatactgcc cggcgttgtc atcctcagac
    33001 aacatcaccg ccgaaagttg agtctgccgc ccgtcagaga cgctgaacat acgcgttccc
    33061 ggcgtgggat tgtgtaccag attcaccaga tccggcgagt gaaagctcac tcgcgcttcc
    33121 ccttgcaaca ctggctgcaa ctggcggtct accgcaccgc cggaaagcgc ggtgcgtttg
    33181 tccagcatct gcatcagcgc cgtactctgc gcctgcccac ccttacgctg gctatgacga
    33241 taatcgagta actgctgctc cagttgcccg aggtagtgat ccgcctgctg caacgtggtg
    33301 agctggtcgt taagctgcac gctgtaacgc tgcgggcgag tggtaatcag cggcgaggcc
    33361 ggatattcac tcgccgtcgc cgggagtttt tgccgcaccg cctgagtggg cgcaacggtt
    33421 gacgacgata ccgcagcgct atgcgcgccg ccggtggcga gtgacgaaga tgtaagtccg
    33481 acctgcatac tgttcctgaa agagttagag aacgctgaac aaattcaact cactcacttt
    33541 cgagtaagtc ttgagggtag cttccattgc cacttccagt ccggtaaagg tgatcgacgc
    33601 cgggccgtaa tcgagatcct gcaatgaacc aatcaactca tcattagagg tagagatatc
    33661 ggtctgcgca tcgctcagca tcgacatggt gttctgcgtc tcgccgagcg acgcaatcga
    33721 ggcgttgaga tcgtccgagg cggtgtccac cacatcaacg gcgttctgaa tatcgctctg
    33781 cacctgcgga tcggccgggt caacgttggg ggcttgcaac tcctgagaga gcgaattgag
    33841 cgtgttgagc acatctaaat tgctaccgaa gaaatcgctc gccgccacgt tggtatccac
    33901 ttccacgccg ttcgaaacgg tggtctggcg gtggtcgctg ttgccctggt agctatagct
    33961 gtcggtcacg ccatcgccgt catcgtccac ggccacaatc ggcggctgat cgttgatggt
    34021 gccgccaaac acgtagtgcc cgtcttcgtc ctgataattg agcgccgcga ccatcgattc
    34081 ggtgagcgac tcaatatcct gcccgaggct tgcgagcgaa tcggcggtat tggtgccgtt
    34141 cgccgcttcc agcaggtcgt cacgcaccgc cagcaggctg ttgttgacgc catcgagaat
    34201 cgactcctgc tggcttaagc ccgccgacgc ggagtcgata ttgctctgat actgctcaat
    34261 cgccgactgc tggcggttta actgggtgat tcgcgtggcg gcgatcgggt catctgacgg
    34321 ctgcaaaatc tgcttgccgg ttgccatctg ctcgaccaca tgcgccagat cgccggagag
    34381 attattaaaa ctttgcgtca tcgagacgta ggtttgttgg gtggttactc gcattatcgc
    34441 gctcccgctt agctgcacat ttccagcacc gaatcgaaaa tctcggcccc ggcggaaatg
    34501 actttcagat tggcttcata aatttgttga taggtgatga ggttcaccgc ctcttcgtcc
    34561 atgctgacac cgctgacgct gctctgctgg ttttgcgctg cggaatagac attggacgcg
    34621 gcatcgactt ccgtctggtt ttgctgactg taaatgccga tattgctgat gattgacgag
    34681 cacgcctgcc cgaccgtcac gctgccgagg ttggcgatct ccagcggttc ggtggagatg
    34741 ttgatcagcg cctgcaggtt gtcgctgttg ccgctttcgt ccggcgaact ggagaacgcc
    34801 agctcatcgg cggtgatatc cgggttgacg gtcagcgggc catcggcatt gctggcgtca
    34861 tagataaaca gcggctcgcc ggggttaccg ttgagatcgt agccctgcgc aagctggttg
    34921 ttgaccgcat cggcaaactg cgacgccatg ctgttgatgg tgtcggtcaa cggcgtcagc
    34981 acatcgtttt gataatcaaa cagtgcgcct aatgaacctc cagtgtcggt ggtcatcgtc
    35041 gaggtggtgc cagcgaaggt cagcgacatg gtcggcgtgc cgtcggcgtt ggtttccagc
    35101 gcgatggtcg aactttgctg cccgctcacc agtggctgac cgtttttcag ggtgacgttg
    35161 tagttgccct gatcgtcgat attgacctgc acatccatca tcccgctgag ttcttccacc
    35221 atctggtcgc gggcgtcata cagcgcagag gcgttatccc cgttggcttc ggcctgcgcg
    35281 atttgctggt tatagctggc aatgccgctg gtgagcgtat tgatttgcga caccatcgcc
    35341 tgctgctggc tgatgatttc cgtgctttgc gagtcgatgt aatcgagcgt gttatcaata
    35401 cgcaacgaca gcgccccggc ttcgctgatc acctgctcgc gcagggcgga atcatcgggg
    35461 ctggtggtcg cttcgttaag ggcggcgaag aagttatcga agccgccgct caggctgctg
    35521 ttatcgtcgc ttagtactgc ttccagttgg ctgagatacc cctgctgggt gctgtaatag
    35581 ccgtaatcgc tggcggcata ccacacctga ttcacctgat actggttgga gacgcggcga
    35641 atgctgtcga cctgtacgcc gttaccggcg ctgttcggcg aaccgccgct ggcaccgatg
    35701 gtgctgatct ccgccacctg acgggtgtag cccgtggtca tggcgttagc ggtgttttgc
    35761 gccgtgacgt tcagctccac ctgcgcggtt gatgcaccgc tgtagccgat gttaatcatg
    35821 tccatgttgg atccttaccg gtaaatcgtg ccggttaaaa gcggcgtaaa gttggggatt
    35881 cttaagttcc gtaaaccggg tgccgaacgc cggatgcggc gtggacgcct tatccggcct
    35941 acatacgggc gactcgtagg cctgataaga cgcgccagcg tcgcatcagg caccgaacgt
    36001 cggagcctca tttatgcttt ggcgacaact gcttcacaat catcgccgcg atcccaatcc
    36061 cgtgctgctg cgccagggtg ttacacaact catcgtcata aaacccctgc atcatgcgca
    36121 ccgaatcgct gttaaatggg ttatctttcg aatcaaacaa ctcgttggtt ttacgcatct
    36181 ctttcagcat gttgcgtaaa aaaatcgcct caaactgttc cgcggcctgc ttaatatcat
    36241 tggcctgaac cttcggcccc attaacgcat cgctgccgtc aatcccgccg ctgccgttga
    36301 ttttcatcag atcacctcca ggtcggcatc cagcgcgccc gcctcatgca gcgcctgtaa
    36361 aatcgacata atgtcgtccg gcgtcgcgcc taaactgttc aacgcattga ccagcgtttt
    36421 caggctgctc gactccggca aactcaccac gcccggacgg gcatgattca ccgcgatatt
    36481 gctctgcggc gttaccgccg tgcgcccgcc cgcaaaggca ttcggctggc tgacgttgct
    36541 ggtttcgtta atcgaaacgg tcaaactgcc gtgcgacacc gccgccgcgt gtagcgcaac
    36601 gccatcgccc atcaccaccg tgccggtacg cgagttaaac accacgcggg cgcggatttt
    36661 ctccgcctgc acctgtacat cgtccagttg cgacataaac gccacccgcg cgccggggct
    36721 ggtgggcgcg cgcacggtaa cgttggtgga gctctgcgcg gtggcaatgc cgccaaacga
    36781 ggagttgatc gccgctgcaa tgttgttggc gtctttaaac gacgggcgtt tcaggttgag
    36841 ggtgatggtg tcgcccatct gaaaatcact ggggatctcg cgctccaccg ttgcgccgtt
    36901 aggaattaac ccggcggtcg gtgtattaac cgttacgctc gaaccgctgg ccccggtggc
    36961 gttcatcccg ccgaccacca cgctgccctg tgccagggca taaacctcgc catctgcgcc
    37021 gtgcaactgc gtcagcagca aggtgccgcc acgaatactt ttcgcatcgc caatcgacga
    37081 gacggtgaca tcaatggtct gcccgcgcga gtacatcggc ggcaacgtgg cgctcaccgc
    37141 cactgcggcg acgtttttca ccttcgggtc gattttattc ggcaattgca cgccgaactg
    37201 gcgcagcata ttggtgatgg tctggttggt gaatttgacc tggtttttat caccggtacc
    37261 gtcgaggccg accaccaggc tgtagccgac cagttggttt tcacgcacgc cctgtacgtt
    37321 aaccagtgac tccagcgatt gcgccagcac cacgccgggt agcgccaggc ttacggctac
    37381 gaccactgtt tttatccagt tttgcattgc actctccggc attaaatcgg gaacagagga
    37441 tggttaaaca accgcgtcag ccagcctgcg gcattggcgt cgcttaacgc gccacgtccg
    37501 gcataggaga tacgggcgtc ggcgatccgc tgggaggaaa ccgagttgtc gttctggatg
    37561 tcgtcggcgc gcaccaggcc gcttaagcgg atgtactcat cgccctggtt gagggtgagc
    37621 catttctcgc cgcgaatttc caggatgccg ttgggctgca ccgcatggac ggaaacggtg
    37681 atctccccgc gcaggctgtt ctgctgctgg gaagtggcgc tgccgtcaaa gtcgcggttg
    37741 gcgtcgatag agccggaaag cttatcttta gtgtgaccaa aaatggtcgg tgcgccgata
    37801 tcgacggtgt tgcttttgcc gaagttggtt ttcgcctgtt tgctcgactg ggtggattct
    37861 tcgaggatca ccgtcaggat atcgcccacg cgataagccc gccgatcggc ggtcagcgac
    37921 cagttgtagc cagtttcaaa tacaccgcca gcacgcccgt ctgccggagg cggtgcttcg
    37981 gttttcggtg gcgcaaagta tgcgtcgtct tttttgacca gaatggcctg cgattcacac
    38041 ccgctaagca ggggaagcag tgccaccagc cagagataat ttttcacgtt ctgcctgttt
    38101 taaatacagt gagttatgtg ctcttgtagg tcggataaga cgcgtcagcg tcgcatccga
    38161 caacaagcgt gtgcacattg cccgatgcgc gtgtggacgg tttataaacg gttaatcaac
    38221 caaactatgt gcaccgtcca ctcgcttatc gtgcctacaa gggcgtcagc gtcgcatccg
    38281 acatttcgcc ttacagcgtc tggctgatgt actgcaacat atcgtccgcc gccgacacca
    38341 ttttggcgtt catctcgtac gcgcgttgca ccgtaatcat cgccaccatt tcgttgacga
    38401 tatccacgtt cgagccttcc agtgcgttgt cctgcaaagt gccaagccca tcttcgcccg
    38461 gcacaccttc tgtcggctga ccgctggcgg cggtttccag atacaggttg tcgccctcgg
    38521 cggaaagccc cgccgggttg gcgaagttaa ccagcgtcag ttgccctaac tcggtcgcgt
    38581 cgctgtcgcc gggtaagatc gccgtcactg tgccatcctc accaaacgcg acgttggtgg
    38641 cccctgccgg aacgtcgatt tccggctgca acggcaagcc ctgcgagttg gtcagcacgc
    38701 cgtcggcgtt cacctgcaag ttgccatcgc gggtataggc gatatcgccg ttagcctttt
    38761 gcacctgcaa aaatccctgc cccataatgg cgacattcat cgcgttgtcg gtggtttcga
    38821 cgttgccttc ggtgaaggtt ttctgggtac cgacaatgcg cacgccgcta ccgaattgca
    38881 gcccggtcgg catgatgttg ttctggtcga gcatcgcgcc cggcgcttcc tgggtctggt
    38941 agaacagatc ctgaaacatc acccggtcgc gcttgaagcc ggtggtatta acgttggcga
    39001 tgttgttggc aatcgcgctc atttcagcat cctgcgccga gaggccggtt ttgctgatcc
    39061 ataatgctgc gttcatggtg gtaaattccg tgttattttt gggccgcggt tgccggatgc
    39121 agcgtgaacg ccttatccgg cctacgatgt gcggttacga gccgcgcaat aagcggttgc
    39181 ccgcgtcgct gatatcttcg gcggttttca tcatcttgat ctgcgcttca aactggcggt
    39241 tcatggcgat ggacgacatc atttcgctca ccgctgagac gttgctgcct tcgagaaaac
    39301 cgccgctgac tttgatattt tcatcgcgct gggccggtac gccgtcggca gtcaccagca
    39361 tcccttcagg attttttgcc aggttagcga ccgggatatc caccagcttc aggcgatcga
    39421 tatccatcgt cgcggtcacg tcgccgtcat cgggcgtcac cgacagcgtg ccgtcgctgc
    39481 caaacgaagc gatggcgttc ggcggcagaa tgatcggccc gttatccccc agcaccagat
    39541 tgccgtcgat agtcagttgc ccctggtcgt cctgctgaat gttgccgttg cgggtataca
    39601 cttcgttgcc gtttttatcc tgcaccgcaa tgtaacctgc cccctgaatc gccacgtcca
    39661 gcgggcgttc ggttttctcc gccacacccg tgctgtcgtt cacgccgctg gccccttcct
    39721 gcgccatata gcgcgtgtcg aagccgccgc ctttcacctt gttgttggtc gccatcgcca
    39781 tatcggcgcg aaaaccgttg gtgttgacgt tcgccaggtt gttggcgcta atttgctgct
    39841 cgtacaaagt ctgagaagcc ccgctcaggg cggtataaat caaacggtcc attacattgc
    39901 ctggaacagc gcgtcatcga gctgtgtgct ggtagcgata actttggtgt tggcctgata
    39961 gttacgctgg gcggtcatca ggttgaccag ttcgctggtg atatcgacgt tagagctttc
    40021 gagcacgccc gatgagagcg tgccgcaagt gccggagcca ggaacgccaa tcagcggggt
    40081 gccggattcc ccggtttgta cccacgcggt gccgctcacc gcttcgagtc cgttttcatt
    40141 cgggaaagtc gccagcacca cctgcccctg caacatgcgc tcgccgttgc tgtaggtggc
    40201 gtaaactttg ccatcgtcat caacctgcac gccgttttgc gttgcggagg cgtagccgtt
    40261 ggcggcattg gtggtgaccg agaagtccga gccgtattgc gtacaagtgg tgtaatcgac
    40321 ggttaagtcg ataggtgcgg cggcgtcggt ctggaactca atggtctgcg gcgtggttgg
    40381 cgaagtcagc ttaccggtgt tcgggtcgaa gattaaggtc gtcgccggaa cgccagtctg
    40441 ctgctgaccg tcgaaggtgt attgcacttc ccaggtgtta tcgctggttt tagtgaagta
    40501 ctggcatacc gagtgttcgt tacccaatga gtcatacacc gtggtggtgt agctgtcggt
    40561 atacgagctg ctgttggttg gatcgaacgg cacggttgta cggtcgatag ccgcatcgct
    40621 ggcatcgaag ttggcagtaa aggtcaggct gctgctggcc tgcgccggga tattgccggt
    40681 tttgatctgg atatccgtca ccgtaccggt ttgcagggtg ccgctgtcat cgaccggata
    40741 accctgtaaa tagtcgcccg aggcgttaac gatatagccg tttttgtcgg tttcaaacga
    40801 tccggcgcgg gtatacgaga tattgcccgc gctgtcgcag gtaacaaaaa acccgtcgtc
    40861 gttaatcgcc agatccagcg cattaccggt ggagatcagc gaaccgccgc gcgaaatgct
    40921 ctgcgcggtg cccgccacac tgacgcccat cgcctggctt ccggcataca tggcggaaaa
    40981 ctgggtagtc atcgacttat agcccaccgt tccggcgttg gcgatgttgt tactgatccc
    41041 gtcgagctgt tcgttaacgg cattcagccc cgttgcggca atttcataac tcattgtgtc
    41101 ttcctgttaa ttcaaatttt gacagcgtgt ccgttgtttt gccggatgcg gcgtgaacgc
    41161 cttatccggc ctacaaaacc atgcagattc aatacattgc agtttcatgt aggcctgata
    41221 agcgaagcgc atcaggcaat ttggcaccgg acatcacgcc acatcactgt cggcatcggg
    41281 tacgccaaac tggctaatca tggtgaacgg tacttcgccc acgcctgcaa cgttgagcac
    41341 cggagagctg ccgtcgagcg ggatgcgcac atcttccact tcgccggaga cttcaatcgg
    41401 cacctcttct tccccggtgt tggtgaccac cgagacgtcg taatcgcccg gcgggatgcc
    41461 gtaatcggcg ggattcaccg agaaatcgac ctggcctggc ccgacggacg aactcccttg
    41521 cgggatcagc gagacggtgt agtcatcgcc tgcggcatcg gtaaagtgga gatcggcagt
    41581 ggtacaggcg tcgtcaagcg tggcgcgacc gttaatggtc tggtcgctca cctgcaaggc
    41641 ggtggtctgc accataatgt cgtcgcccac caggttaccg agcgccatca cctggatgtt
    41701 gctcatcagc accgcattgg tattcgcctg cacgctcatc tcttccatca tcgccacctg
    41761 cgccatcgac gacagctggt cgatatattc ggtagcgtcg gtcgggtcgg ttgggtcctg
    41821 gttttggatc tcggcgacaa acagcgtcag gaaagtatcg acgggcgagc tatcgctgct
    41881 gctttgcgtg tcgctggccg ccacgttgtt gctttccgcc gtggcggtgg gttgcggcga
    41941 ttgcgcattc agcgccaggg tattcatcag gcttctccca gtttcagcaa actttgctgc
    42001 atacttttca cgttgttgag cacatcgaca ttggtttcga aatcgcgcga ggccgacatc
    42061 atgtccgcca tctgttccac cacgttgacg tcgggatacc agacatcacc gttagcatcg
    42121 gcgagcggcg aatgcggttc atagcgtttc accgccccgc cggtttgcag cacgtcctgt
    42181 acctgcacgc tggcaccgtc aatggcatga cgatgtgtac ccgccagcag gctgtggtga
    42241 tacaccgcgg caaacaccgg gctgcgcgct ttatacgcct gcgcctcgct gctggcgggc
    42301 gcattggcat tcgccaggtt actggcaacg gtattcagac gtagcgtttg cgccgtcatc
    42361 gccgagccgg aaatctgata gatatcagta aatgacatgc ttatttccct tcaatggcct
    42421 ctctgatacc gctgatttgc agattcagaa aggtcaaact gctttgataa tccatactat
    42481 tttgtgaaaa ccgcgcctgt tcgctgttca gttccacggt gttgccatct tccgacggct
    42541 gcatcggcac acggtactgg acatccacca ccgcgttatt tgcccgttgc atctccctgg
    42601 caaaatcaat atctttagcc ttgaaatttg gcgtatcgac attcgccaga ttcgcagtca
    42661 gtagctcggt cctctcaaga cgtaacttca ctgcctgcgg atgtactccc aatgcctgtt
    42721 gaaaactgat ccccatttcc tgcgtcactc ctgcttcaat ccgcatacgc taagcaatcg
    42781 ccatgccatt ttttaagtca ctgatgagaa agggtttatg agttttcaat tttgcaggcg
    42841 gaagttttat ttccttctgg cgctagcctt accgggctac gccgccgtcc atcccgtgca
    42901 acacagcgcg cgggaacaag tcaacgccca ggtgctgaat gccgcgagcc aaaaaattga
    42961 atcgctggcg cagcagcggc agtggcatga ctatcgctac acctttaaga tctacattcc
    43021 ctcgcagatt gcgaccgccg cgccctgtac taaaacaccc ggcgtcacgc tgacttcgcc
    43081 tgcggaaatc gccctcaacc ggatgaactt caccgtcagt tgcccgcaaa gctggcagat
    43141 gaacgtggcg gtgcgcccgg atgtactggt gccggtggta atgacgaagt cactggttgc
    43201 ccgcgatacg ccgctcaccg cgaatgatgt ggagcttaag ccctacaacg tcagcgccca
    43261 gcgccgcgat gtgttgatgg ttctcgatga cgcgattggt ttcagcagta aacacgccct
    43321 gcaacccggc aggccaatca ccaaagaaga actcgtgtct ccggtgctgg tcgggcgcga
    43381 tcaaccggtg atgattgttt accagagcgc ggggatcacc gcctcgatgc ccggcgtggc
    43441 gctgaaaaat ggccgcaaag gcgagatggt gaaagtgcgt aacgccagca gccagcgggt
    43501 catcagcgca atggtggcgg aaagcggagt ggtgacaacg gtaagcgcgg agtaatttgc
    43561 gctaacgatt aaagttttgc cgcctgttaa ccgctttacc gcagtaaccc acccaattac
    43621 cgagacgagg ttgcgatgaa aatcaccccg actatgccag gcaatcgccc gacagccaca
    43681 acggcaggct ccagccagcc gcgcaaaacc acggcgacca ccaccacgac gctgagcgcc
    43741 gacgacatta ctcaggcggg attgcagtcc gcccagcaaa cgctgaacga ccagcaagag
    43801 agcgatatcg acagcgacaa agtggcgcaa atgcaggcga tgctggcctc tggcagtcta
    43861 caagtggaca ccgaacagtt agccagcgac atgctgagtt ttttccagaa ctaagaggcc
    43921 gatgaagtga gtatccggtt acaacaggtg aaggcgttgt tgcagggaat tcgtgccgac
    43981 gacgctctgt atgattccct gcgcgaactt cttcagcgcc agcggatctg catgattcgc
    44041 cgcgccagcg aagagttgct ggcggtaaac gaggagataa cccatcacta tgaacagttg
    44101 catgggcata gccatcagcg gcatagcctg ctgaaaatgc tcggtgttag cgtaaatcgt
    44161 gacggtctgg cgcaggtgtt tgcctggctt ccggcggtcc aaaaagcggc agcacaacaa
    44221 ttgtggcaac gccttgagca aaaagcggaa cgctgcaaaa cctacaacga taaaaacggc
    44281 gagctgttga ttcgccagta tgaatttatt cagtcatttt taggcagcga agcagatttt
    44341 ctctatcagg aataacgcgc ataccttccc catagcggaa acggattttc cgcctgattc
    44401 tcaatccttt gtttttgatt acaaaaaatc tcggcacgct tattgcacat tctgcacagc
    44461 attaaagact ggaagccacc tatgagtttt attgaatgtt acgaaccgga atacgtgcgc
    44521 aaatttttag cgcgtcaccc cggttcgcct ttatacgtcc gcaaggtgtg gaaaaacgag
    44581 attcgcctga gcctgtcatt gaccgatatt gccagctgcg aagcggtgct caacgatgtc
    44641 agggcctttg atttacagct cacctaccgt cctgtggaag aaaatgccgc agagctttcc
    44701 gcgcgcgatg cgatagtaaa ccaggtgatt ttgagtaccc tcacgctccg tgatttaaca
    44761 ccagaactgt cattgtatgc cgtcggtatc ctgctctccc gcgccagtaa aatgccgggc
    44821 agagacggag atattcttgc ccgtttgacg acgctgccgc aggcgctggc cgatcatgcg
    44881 aaaaaaggca tcctacaggc acagttcgcc cagctgccgc ccgtgccaca gctggcgcgt
    44941 cacctggcca cgttactcgg gagttttacg tttgactggt cgattttgcc ggaaagcccg
    45001 cgcaaaactt cgctgccgtt gcagatgcct ttgctgacgc tgcatgacgc taacagcgaa
    45061 gccctgttac aacagcagct tcagacgcag tggcaaacca cctggcaaca gcattttgcc
    45121 accgcgccct ggatgatgcg taactggctg atttaccgcg tttaccacga tgtgattggg
    45181 cagactgacg gggcggatta tttcccgctg gtgtgcgatt tttatctcct ccgcacgtta
    45241 atcagtttat ggacgctgga tggttcttcg ctgcggcagg aggatatttt cgccctcttt
    45301 gccatgtttg aacgctggcg agccagtgaa aatgcgctgt tggttcgcca gcaaattcag
    45361 tcgttatgtg ccgccgaccc gctgctttca gcgttttcgc tgctaacgtg agtcgctctg
    45421 tgcgcgcttt
//
LOCUS       DAJOSY010000022        44833 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJOSY010000022 DAJOSY010000000
VERSION     DAJOSY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.022"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(43..330)
                     /locus_tag="OHN27_001375"
     CDS             complement(43..330)
                     /locus_tag="OHN27_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCQ2285851.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(405..548)
                     /gene="mgrB"
                     /locus_tag="OHN27_001376"
     CDS             complement(405..548)
                     /gene="mgrB"
                     /locus_tag="OHN27_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCQ2285852.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            707..946
                     /locus_tag="OHN27_001377"
     CDS             707..946
                     /locus_tag="OHN27_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285853.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1089..1880)
                     /gene="kdgR"
                     /locus_tag="OHN27_001378"
     CDS             complement(1089..1880)
                     /gene="kdgR"
                     /locus_tag="OHN27_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCQ2285854.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2057..3430
                     /locus_tag="OHN27_001379"
     CDS             2057..3430
                     /locus_tag="OHN27_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2285855.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3476..4357)
                     /gene="htpX"
                     /locus_tag="OHN27_001380"
     CDS             complement(3476..4357)
                     /gene="htpX"
                     /locus_tag="OHN27_001380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCQ2285856.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4549..6597)
                     /gene="prc"
                     /locus_tag="OHN27_001381"
     CDS             complement(4549..6597)
                     /gene="prc"
                     /locus_tag="OHN27_001381"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCQ2285857.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6617..7315)
                     /gene="proQ"
                     /locus_tag="OHN27_001382"
     CDS             complement(6617..7315)
                     /gene="proQ"
                     /locus_tag="OHN27_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCQ2285858.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7412..7909)
                     /gene="msrC"
                     /locus_tag="OHN27_001383"
     CDS             complement(7412..7909)
                     /gene="msrC"
                     /locus_tag="OHN27_001383"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCQ2285859.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8039..9322
                     /gene="yebS"
                     /locus_tag="OHN27_001384"
     CDS             8039..9322
                     /gene="yebS"
                     /locus_tag="OHN27_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCQ2285860.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9291..11924
                     /gene="yebT"
                     /locus_tag="OHN27_001385"
     CDS             9291..11924
                     /gene="yebT"
                     /locus_tag="OHN27_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCQ2285861.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPSEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12004..13443
                     /gene="rsmF"
                     /locus_tag="OHN27_001386"
     CDS             12004..13443
                     /gene="rsmF"
                     /locus_tag="OHN27_001386"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCQ2285862.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQQVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13561..13797
                     /locus_tag="OHN27_001387"
     CDS             13561..13797
                     /locus_tag="OHN27_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCQ2285863.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13902..14093
                     /locus_tag="OHN27_001388"
     CDS             13902..14093
                     /locus_tag="OHN27_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCQ2285864.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14094..14750)
                     /gene="pphA"
                     /locus_tag="OHN27_001389"
     CDS             complement(14094..14750)
                     /gene="pphA"
                     /locus_tag="OHN27_001389"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCQ2285865.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTSAVFGGELTLVQLQ
                     "
     gene            complement(15145..15486)
                     /locus_tag="OHN27_001390"
     CDS             complement(15145..15486)
                     /locus_tag="OHN27_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCQ2285866.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQSISVIQIDDSNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15559..16371)
                     /gene="copD"
                     /locus_tag="OHN27_001391"
     CDS             complement(15559..16371)
                     /gene="copD"
                     /locus_tag="OHN27_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCQ2285867.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFFLLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLE"
     gene            complement(16375..16749)
                     /gene="yobA"
                     /locus_tag="OHN27_001392"
     CDS             complement(16375..16749)
                     /gene="yobA"
                     /locus_tag="OHN27_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCQ2285868.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQHPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16888..17118
                     /gene="holE"
                     /locus_tag="OHN27_001393"
     CDS             16888..17118
                     /gene="holE"
                     /locus_tag="OHN27_001393"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCQ2285869.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17220..17876
                     /locus_tag="OHN27_001394"
     CDS             17220..17876
                     /locus_tag="OHN27_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCQ2285870.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17900..18562
                     /gene="exoX"
                     /locus_tag="OHN27_001395"
     CDS             17900..18562
                     /gene="exoX"
                     /locus_tag="OHN27_001395"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCQ2285871.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18559..20619)
                     /gene="ptrB"
                     /locus_tag="OHN27_001396"
     CDS             complement(18559..20619)
                     /gene="ptrB"
                     /locus_tag="OHN27_001396"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCQ2285872.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRNGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPESETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINERPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20828..21487)
                     /locus_tag="OHN27_001397"
     CDS             complement(20828..21487)
                     /locus_tag="OHN27_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCQ2285873.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     VNKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21814..22170)
                     /locus_tag="OHN27_001398"
     CDS             complement(21814..22170)
                     /locus_tag="OHN27_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCQ2285874.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22237..22527)
                     /gene="yebG"
                     /locus_tag="OHN27_001399"
     CDS             complement(22237..22527)
                     /gene="yebG"
                     /locus_tag="OHN27_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCQ2285875.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22661..23839
                     /gene="purT"
                     /locus_tag="OHN27_001400"
     CDS             22661..23839
                     /gene="purT"
                     /locus_tag="OHN27_001400"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCQ2285876.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSEEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSEN
                     VTFDNVQNAVGAGLQIRLFGKPGIDGSRRLGVALAAAESVDEAIERAKHAAGQVKVQG
                     "
     gene            complement(23895..24536)
                     /gene="kdgA"
                     /locus_tag="OHN27_001401"
                     /gene_synonym="eda"
     CDS             complement(23895..24536)
                     /gene="kdgA"
                     /locus_tag="OHN27_001401"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCQ2285877.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24573..26384)
                     /gene="edd"
                     /locus_tag="OHN27_001402"
     CDS             complement(24573..26384)
                     /gene="edd"
                     /locus_tag="OHN27_001402"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCQ2285878.1"
                     /translation="MNPQMLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26619..28094)
                     /gene="zwf"
                     /locus_tag="OHN27_001403"
     CDS             complement(26619..28094)
                     /gene="zwf"
                     /locus_tag="OHN27_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCQ2285879.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28432..29301
                     /gene="hexR"
                     /locus_tag="OHN27_001404"
                     /gene_synonym="yebK"
     CDS             28432..29301
                     /gene="hexR"
                     /locus_tag="OHN27_001404"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCQ2285880.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29429..30871
                     /gene="pyk"
                     /locus_tag="OHN27_001405"
     CDS             29429..30871
                     /gene="pyk"
                     /locus_tag="OHN27_001405"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCQ2285881.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31002..31973)
                     /gene="lpxM"
                     /locus_tag="OHN27_001406"
                     /gene_synonym="msbB"
     CDS             complement(31002..31973)
                     /gene="lpxM"
                     /locus_tag="OHN27_001406"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCQ2285882.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32093..33415)
                     /gene="mepM"
                     /locus_tag="OHN27_001407"
     CDS             complement(32093..33415)
                     /gene="mepM"
                     /locus_tag="OHN27_001407"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCQ2285883.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33431..34363)
                     /gene="znuA"
                     /locus_tag="OHN27_001408"
     CDS             complement(33431..34363)
                     /gene="znuA"
                     /locus_tag="OHN27_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCQ2285884.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34442..35197
                     /gene="znuC"
                     /locus_tag="OHN27_001409"
     CDS             34442..35197
                     /gene="znuC"
                     /locus_tag="OHN27_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCQ2285885.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVHAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35194..35979
                     /gene="znuB"
                     /locus_tag="OHN27_001410"
     CDS             35194..35979
                     /gene="znuB"
                     /locus_tag="OHN27_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCQ2285886.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36126..37136)
                     /gene="ruvB"
                     /locus_tag="OHN27_001411"
     CDS             complement(36126..37136)
                     /gene="ruvB"
                     /locus_tag="OHN27_001411"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCQ2285887.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37145..37756)
                     /gene="ruvA"
                     /locus_tag="OHN27_001412"
     CDS             complement(37145..37756)
                     /gene="ruvA"
                     /locus_tag="OHN27_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCQ2285888.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATNDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(37895..37960)
                     /gene="yobI"
                     /locus_tag="OHN27_001413"
     CDS             complement(37895..37960)
                     /gene="yobI"
                     /locus_tag="OHN27_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCQ2285889.1"
                     /translation="MYIFITHFFTEYVILKYLLPK"
     gene            38031..38633
                     /locus_tag="OHN27_001414"
     CDS             38031..38633
                     /locus_tag="OHN27_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCQ2285890.1"
                     /translation="MNINYPAEYEIGDIAFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(38635..39156)
                     /gene="ruvC"
                     /locus_tag="OHN27_001415"
     CDS             complement(38635..39156)
                     /gene="ruvC"
                     /locus_tag="OHN27_001415"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCQ2285891.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(39191..39931)
                     /locus_tag="OHN27_001416"
     CDS             complement(39191..39931)
                     /locus_tag="OHN27_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2285892.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(39960..40412)
                     /gene="nudB"
                     /locus_tag="OHN27_001417"
     CDS             complement(39960..40412)
                     /gene="nudB"
                     /locus_tag="OHN27_001417"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCQ2285893.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(40530..42302)
                     /gene="aspS"
                     /locus_tag="OHN27_001418"
     CDS             complement(40530..42302)
                     /gene="aspS"
                     /locus_tag="OHN27_001418"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCQ2285894.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            42612..43178
                     /locus_tag="OHN27_001419"
     CDS             42612..43178
                     /locus_tag="OHN27_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCQ2285895.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            43175..43669
                     /locus_tag="OHN27_001420"
     CDS             43175..43669
                     /locus_tag="OHN27_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCQ2285896.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIF
                     IRHV"
     gene            43723..>44833
                     /locus_tag="OHN27_001421"
     CDS             43723..>44833
                     /locus_tag="OHN27_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2285897.1"
                     /translation="MKRVISYIRFSSERQAKGDSLRRQSKMVNDWLKAHPEYYLDQDL
                     SFRDLGVSGFTGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDVGQASELLR
                     NILRSGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRSHEESLRKSERIQAAWDAKK
                     EAAKAGIKVSKRCPAWLKLNDDRKSFSVIPEKVETVKRCFQLRLEGKSIWEIVRTLND
                     EGHQTLNQYKAGKFGQTSVQDLLKNRSVIGYFTPKGCEEIAGYFPPIIPETDFYKVQQ
                     LSYSRYGRKPASDKPLSVNLFKGVIRCSECGHALIITGFSDTRSGIYRCPSRNENRCN
                     AKGLSRKQTDGTLLALLSRIDRFDSDMTNTIDTLKL"
BASE COUNT        10747 a        11607 c        11037 g        11442 t
ORIGIN      
        1 agtaaccctt caacgacggg gttactcaag attgaggaag gattatcgtt cggcgaccag
       61 acgaataata tcttcgtctt caaccgattt ttgactctca acggtctttt tatctgcttt
      121 ctcaggttcg tttgcttcgc acaaacgacg taacagtgca ttttgacgtt tttgctgatc
      181 cagcaacgcc tcaagcagtt caatctgttc gttagtccga gaactggcac gattgataaa
      241 aaaccacaag atgagcccga taagaagaac caccaccgat acaaccaaag acgcaatgtt
      301 catcacgcct gaatttacaa cttcgttcat ttcaccacct ctgagtagag gcgctattct
      361 accactgctg gagaggaaga aaatctagtg ctgaaaaaat gatatcacca cgggataaac
      421 tggttaatgg cacaaattcc gctgaaaaat tgtacatcct gatcgcacat catgttgaat
      481 acctgcgccc aaagcagcaa gcaagccaac accacgacaa ccagaacgac ccatcgaaac
      541 tttttcactc caatctccgc ttcacctacc ttatgtcatt aaactagcat gcgttagtta
      601 aacagcgcct aactatgtcg atattcgtgc tttttcggaa tgagtagctt gcttcagcgc
      661 atattggcgc tacgattaag cgaacataaa aagagaagag gttgtaatgc gattcatcat
      721 tcgcacagtt atgctgatag cactggtatg gataggatta ttactcagcg gatatggcgt
      781 tttgattgga agcaaagaga atgccgcagg attaggtttg caatgtacct atctgactgc
      841 cagaggcacc agcacggtac aatacttgca cactaaaagt ggtttcttgg ggataacaga
      901 ttgcccactg ttacgtaaaa gcaatatcgt ggtcgataat ggttaagggt aataaataac
      961 cagattctca ctcatgagta aaatattctt atagcaagcg cgtttaatat aacgcacctt
     1021 caactttatt tttctcatgc taaataaaga acctgtctgt ttgatattcc gacaggttct
     1081 tactctcatc agaacggata gtcgtgataa cccatttggg cagaaatttt gcgcgctgcg
     1141 gtatgcaaca ttgcgacata ttcctgtaaa cgctcttcag agaaacgcaa cgtcgggaag
     1201 gagatgctca aaccggcaat gaccacgcca aagcgatcaa ataccggtac cgcaatgcat
     1261 cgcagccctt cttcctgctc ttcgttatct tcgccatacc cctgctcgcg cacctggtcc
     1321 agaacgggta ataacgcttc tgtactggtg atggtccgct cggtactgcg tttatactcc
     1381 acgccctcaa gaatttgctt cacttcatcg cgatcgcgcc atgccagcag taccttacca
     1441 atcgcggtgc tgtacagcgg attacgacgc ccaatccgtg aatacatgcg caaattgtac
     1501 atagagtcaa ttttgtgaat gtaaacaata ctgtcttcgt ccagtgcgcc gaggtggata
     1561 gtttctttgg tcaggcggga gatctcacgc atctggatat ctgcgctacg aattaaatcg
     1621 acgttttgta acgcgcgagc gcccagttca aacaatttca gggtcaggga atatttctcc
     1681 gactcccctt cctgcgccac ataacctaag gttttcatgg tctgtaaaaa gcgataaacg
     1741 gtgctttttg acatcatgac gcgctgcgac agctcggtta tccctatttc gcgctcttca
     1801 cccagcgcct gcaaaatgcc aaaaactttt agcacggaag atacagaatc aggctgttta
     1861 tccagatctg cgttagccat ttatcacctc attgcgagtg ttttataaaa atcagaactg
     1921 ttttttatta taatttcgca ccagggtggt cgcaatccat cttttgccgg ttagttacaa
     1981 ttctgcgaca tccaccgtga atatcagtgc tagaatcata cccctgttga ttattcacca
     2041 aagatataaa attcctatgc caaaagttca ggccgacggc ctgccattgc cccagcgata
     2101 cggtgcgata ttaaccattg tgattggtat ttcgatggct gtccttgacg gcgcaatcgc
     2161 caacgtcgcc ctgccaacaa tcgccacgga ccttcatgcc acgccagcca gttccatctg
     2221 ggtagtgaac gcctatcaaa tcgccattgt catctccctg ctctcgtttt cgtttctggg
     2281 cgatatgttt ggctatcgac gtatttataa atgcggtctg gtcgtttttc tgttgtcttc
     2341 actgttctgc gccctttctg attcgctgca aatgctcacc cttgcgcgtg tcatacaagg
     2401 tttcggcggt gcagcgttga tgagcgttaa taccgcactt atccgcctga tctatccaca
     2461 acgttttctg gggagaggga tgggcataaa ctcgtttatt gttgccgtct cttctgctgc
     2521 cgggccgaca attgctgcag caatcctctc catcgcatcc tggaaatggt tatttttaat
     2581 caacgtaccg ttaggtatta tcgccctgct tctggcgatg cgttttctgc cacccaatgg
     2641 ttctcgcgcc agtaaacccc gtttcgacct gcccagcgcc gtgatgaacg cgttaacctt
     2701 cggcctgctt atcactgcgt tgagtggttt cgctcagggg caatcgctga cgttaattgc
     2761 tgcggaactg gtggtaatgg ttgttgttgg tattttcttt attcgccgcc agctttctct
     2821 tcccgtaccg ctgctaccgg tggatttact gcgtatcccg ctgttttcac tttctatttg
     2881 cacatctgtt tgctctttct gcgcacaaat gctggcaatg gtttccctgc ccttttacct
     2941 gcaaaccgtg ctcgggcgta gtgaagtcga aacaggttta cttctgacac cgtggccgtt
     3001 agcaacgatg gtgatggctc cgttggcagg ctatttgatt gaacgcgtac atgcaggatt
     3061 gctgggtgct ctagggttgt tcatcatggc tgcggggctt ttttccctgg ttctgctgcc
     3121 cgcgtcacct gcggatatca atattatctg gccgatgatc ttatgtggcg ctggatttgg
     3181 cttgttccag tcacccaata accacaccat tattacctcc gctcctcgcg aacgtagcgg
     3241 tggagccagt ggcatgttag gaacggctcg tctactgggt cagagtagcg gcgcggcact
     3301 ggtggcgctg atgctaaatc agttcggtga taatggtacg cacgtttcgc tgatggctgc
     3361 ggctattctg gcagtgattg ctgcctgtgt cagtggttta cgtatcactc agccacgatc
     3421 cagggcataa aaaaagcgcg tcgatcagga cgcgcttttt agtatttact tcatattact
     3481 tcaggtattc acccgtacgc agagcttcaa ttcgtttatc cagcggcggg tgagtcatga
     3541 acaactcact gagcgatttc gacttaccgt taatgcagaa agccatcatg ctggttgctt
     3601 cttgcggttc atagctggtt ttcaggcgct gcagcgcggc aatcattttc tcgcgaccaa
     3661 ccagttttgc cgaaccggca tcagcatgga attcacgatg acgcgagaac cacatggtga
     3721 taatgctcgc cagaatacca aacaccagtt ccagaaccgt tgcaaccgca aagtagatca
     3781 gcgggttgcc gttgctctct tcaccttcat cacgatttcc gcccataaaa cccgcggcaa
     3841 gctgcgccag aatacgggaa ataaagataa cgaaggtgtt caccacgccc tgaatcagcg
     3901 tcatggtaac catatcacca ttggcgatgt ggctgatttc gtgagcaatt accgcctcgg
     3961 cttcatccgg gctcatgttc tgcagcaaac cggtgctgac agcaaccaga gaggcatcac
     4021 ggcgcgcacc ggttgcaaaa gcgttgatgt ccggcgcatg gtagatagcc acttgcggca
     4081 tagcgatccc tgcctgacga gcctgggttg ctacagtatt gaccagccaa cgttcccttt
     4141 cgttacgcgg ttgctcgatc acttccccgc caacagatcg taatgccatc catttggaca
     4201 tcagaagcga aacgaaggaa ccaccaaaac cgaacagcaa ggccatgatc atcagcccct
     4261 gaacgctgct cgactgtatc cctgtcaggc tcagtaccag cccgaaaacg accattacgg
     4321 ccaggttcgt tagcaggaag agcgcgattc gcatcataat tttcttttta cctcagttta
     4381 acaaaacgca atatgcgata cccacatcgt atgggttacg cgactatttt caagtctgga
     4441 tagtgcgtaa gtcaccagaa agacacaact ttacattttg tagcatctga tttacggcat
     4501 cttgccgctg ttaaaaaatc aggcacaatt tcttgtgcct gattgatatt acttgacggg
     4561 agcgggttgt tccgcgggtc tggctttttc aagcttcgcc agatcgagtg cgatattcac
     4621 cgtctcatcc agataaggat ccggctcctg gtaatctttc ggtagatcat ccagcttctt
     4681 caactccggt ttaccttcgc gtttaaagcg ttcgttcaaa cgcgccagac gcgtcgcatc
     4741 atcttcatta ttctctttct cacgcacagc gtagttcaga gaaacgatat tgcgcttgtc
     4801 cttcatagcg ttgaagcgcg cgatatcctt catgatgttc tggaactcag gatctttcgc
     4861 gatacgcgca ttatgttcct tcagcagctc cggcccaaag gccgttaaat cgcctgattt
     4921 cacataagtc gcggcatcaa tgctatccca cggcagcgcg ttatcttcga atttctcacc
     4981 cgtttccgtt tcttcattac ccgtcggcat gatgatgtct ggcgttacgc ctttacgttg
     5041 cgtactgccg ccgttaacgc gatagaattt ctggatcgtg tactgcacag aacccagcgc
     5101 tggccattca ggacgtaaca tctgatcgta aatacggttc aatgaacggt attgctgaac
     5161 ggtgccttta ccaaacgtcg gttcacccac aaccagcgca cgaccgtaat cctgcattgc
     5221 cgcggcaaag atttctgaag ccgaagcact gaaacggtca accagtacca ccagcgggcc
     5281 tttatagaac acctgcccgt cggtatcgct gtcttcacga accttgccgt tgttatcgcg
     5341 gacctgaaca atgggacccg caggaataaa cagacctgag agcgataccg cttcggtcaa
     5401 cgccccaccg ccattgctac gcaggtcgat gatcacactg ctgacattct gtttttccag
     5461 tttctgcagt tgaactttga catcgtctgt caaacccaca tagaagcccg gaatatccag
     5521 cacgccgact ttctctttac cgacggtctt caccgacatt ttaaccgcgc ggtcttcgag
     5581 acgaatacgt tcacgggtca atgttacagt gcgggtcttg gtccctttac cagcaggtaa
     5641 aatttccaga cgaactttac tgcccttcgg ccctttaatt aaggcaacca catcatcaag
     5701 acgccagcca atcacgtcaa ccatcggctt gcctgtttga ccaacaccga caattttgtc
     5761 accaacgctg atagctttac tcttcgctgc cggaccacct gccaccatcg aattgataac
     5821 ggtgtagtca tcatccattt gcagcactgc gccaatacct tccagcgaca aactcatttc
     5881 agtgttgaac tgttcggtat tacgcgggga aagatagttg gtatgcgggt cgatttcacg
     5941 cgcaaacgcc gtcattgcca gcgagaaaac atcttcgctg ttggtttgcg ccagacgacg
     6001 aatggcaaat ttgtagcggc gtgtcagggt ttcacgaatt tctttatccg tttttcctgc
     6061 cagcttcagg cttaactcgt cgaatttgac tttactgtcc cacagcgcgt tcaactcagc
     6121 ctcgtttttc ggccagggcg ctttgctgcg gtcaaggtta taagtgtcgt tgccggtgaa
     6181 atccatcggc ttttccagta ccgacaaagc gtactggtaa cgttcaaaac ggcgcttttg
     6241 cgccagattg tagagatcgt agaaaacgtc gagtttgcct gaacgcaatt catcgcctaa
     6301 ctcggttttc tttttcgcga actgttcaac atcgcttgcc agcagcacat tgtggctgta
     6361 atcgagcaga ttcaggtagc ggtcaaagat tttggccgaa aatgcctgat cgaggtcgaa
     6421 ctggcgataa tgagaacggg tgaagcgcga cgttacgcgc tcacttaccg tcgcatgctg
     6481 cgtctcttcc tttaataccg gaatttgatc agcacgcgtg atatcttcta cagcgaaggt
     6541 ctggcctgct attgcaagca ggccagctaa cgcggtaagc ctaaaaaaca tgttcatgcc
     6601 tggcccggcc tccgtttcag aacaccaggt gttctgcgcg cacaatcaaa gacatacccg
     6661 aattcagctg gacgcggacg ccgtctttgg tgatttctaa tacggtggca tccatcgcgt
     6721 tttgacccgc tttcaccttc agggcttgtc cgacagtcag agctgaaatg tcagaaaccg
     6781 gggtgtgctg ttcttcgcga ggtgctttta ctgtttttgg cgctttctct accggctttt
     6841 gcgcacgagg tttacgttca gcgccttctt tgcggcgtgg cgtggtcgga cgtggcttgc
     6901 gttcgcggcg cggtgcgtct tctttctcac cagcagctgc ggcagcttcg cgttttttcg
     6961 cttgctgttc agcacgctgt gcctgaacac gcgctttcgc ttcttcaagc tgcttgcgag
     7021 catgctctac atgttgctcg tccagctcac cgcatgggtt gccgtcaaga tcgacacgcg
     7081 ttgcgccggg tttaacaccg taaagataac gccagctcga agtgtagaga cgtaaagcgg
     7141 atcgtaattg cgttttgctc aggttcattt ccccggcaac acgatcgacc aaatcctgaa
     7201 aaataccgat tttcagcgga cgcgcttcac cttccgcact gaaacagtgg ggaaaacgtt
     7261 cggccagaaa cgcgattact tctttactgc tattcaactt aggttgattt tccatgaaat
     7321 ttcctgatta caacggacgt agccaacaag cgcaggcatg aacaggcgtc attataatga
     7381 cgctaccagt aaatgctacg ttatccgttg attatcctgc gacgctcgca aagaattttt
     7441 tgtaatccgt cgttgcaagc actttttcaa gctgtgccac aagctgacgt aagccttgct
     7501 cgtcctcgtc tgtaaagcga ccgaagacgg tactatcgat gtcaagaaca ccaataatct
     7561 gatttttcac caccagcggc agaacaattt cagaattact cgccgcatca caggcaatat
     7621 gcccgtcaaa cgcgtgaaca tcctcgatac gctgcacttg attgcgggca accgcagtgc
     7681 cgcacacccc gcgcccgacg ggtatccgga cacaggcaat tttgccctga aatggtccga
     7741 gtaccagtgt atcgtcctca agcaaataaa aacctgccca gtttacgtca gtgagacgct
     7801 cgtataacaa cgcactggtg ttcgcaagcg ttgccagaaa actggtttct cccgccatca
     7861 gcgcgttaaa gtcgcgattt aaatccgcgt aaaactctgt tttgttcatt atataatcac
     7921 ttgtttgtct tacctggaac tgccagccta ttaaaataag cattaaatgc gttaatgctc
     7981 aagatcattc ccatcatggg ttaagattaa tgttaattct tattacattt ggcacgtcat
     8041 ggctcttaac acaccacaaa ttacgccgac aaaaaagata acagtgaggg caatcggcga
     8101 ggaactgccg cgtggtgatt accaacgttg cccgcaatgt gacatgctgt ttagcctgcc
     8161 cgagataaat tctcatcaga gtgcctattg tccgcgctgt caggcaaaaa ttcgcgacgg
     8221 gcgcgactgg tcgctaacgc gcctggcggc aatggctttc accatgctgt tgctgatgcc
     8281 gtttgcctgg ggcgaaccgc tgttgcatat ctggctgtta ggtattcgta tcgacgccaa
     8341 cgtcatgcaa ggcatctggc aaatgaccaa acagggcgat acgataacgg gggcgatggt
     8401 ctttttctgc gttattggtg cccccctcat tctggtgtcc tccatagcat atttatggtt
     8461 tggtaaccga ctgggaatga atttacgtcc ggtactgctg atgcttgagc gacttaaaga
     8521 gtgggtaatg ctggatatct acctggtcgg cattggcgtt gcttctataa aggtacagga
     8581 ttatgcccat atccaggcgg gtgtcggctt gttctctttt gtggcgttgg tgattttaac
     8641 gacggtgacg ttgtcacatc ttaatgtcga ggagctgtgg gagcgatttt atccgcagcg
     8701 ccccgctacg cgtagggacg agaaacttcg tgtctgtctt gggtgccatt ttaccggcta
     8761 tcctgatcag cgtggtcgct gcccgcgttg ccatatcccg ctacgcctgc gtcgccgtca
     8821 tagtttgcaa aaatgctggg cggcgctgtt agcgtcaatc gttttgttgt tacctgccaa
     8881 cctgttgcct atttctatca tttatctgaa cggaggacgg caggaagata caattctttc
     8941 cggaattatg tcgctggcaa gtagcaacat tgcggttgcg ggaatcgtgt ttatcgccag
     9001 tattctggta ccgtttacta aagtgatcgt catgttcact ttactgttga gcattcattt
     9061 taaatgccag caaggtttac gcacacgcat tctgttactg cggatggtga cctggattgg
     9121 tcgctggtcg atgctcgacc tgtttgtcat atctttaacc atgtcgctga ttaatcgcga
     9181 tcagatcctc gcttttacta tgggaccggc tgcgttttat ttcggcgcag cggtaatttt
     9241 gactattctt gctgtggaat ggctggacag ccgcttactt tgggatgcac atgagtcagg
     9301 aaacgcccgc ttcgacgact gaagcgcaga ttaaaaataa acgccgtatc tcacctttct
     9361 ggctgctgcc gttcatcgcg ctaatgattg ccggttggct gatttgggac agttatcagg
     9421 accggggtaa taccgtcacc atcgacttta tgtcggcgga tggtattgtc ccgggccgta
     9481 cgcctgttcg ttatcagggc gttgaagtcg gaacagtgca ggatatcagc ctcagcgacg
     9541 atcttcgtaa gattgaagtc aaggtcagca tcaagtccga tatgaaagat gcgctgcgcg
     9601 aagagactca gttctggctg gtgacgccaa aagcatcgtt ggcaggtgtc tccgggctgg
     9661 acgccctcgt cggtggtaac tatatcggca tgatgccagg taaaggtaaa gagcaggatc
     9721 actttgtcgc actcgatacc caaccgaaat atcgactgga caatggcgat ctgatgatcc
     9781 acctgcaagc ccccgatctc ggttcgctga acagcggttc attggtctat ttccgcaaga
     9841 tccctgtggg aaaagtctac gactatgcca tcaatcccaa caagcaaggc gtggtgattg
     9901 atgtcctgat cgagcggcgt tttaccgacc tggtgaaaaa aggtagccgt ttctggaacg
     9961 tttccggcgt tgatgccaac gtcagtatca gtggcgcgaa ggtgaaactg gaaagtctgg
    10021 cggcactggt taacggtgcg attgccttcg attcacctga agagtcgaaa cctgccgagg
    10081 cggaagatac ctttggtctg tatgaagatc tggcccacag ccagcgtggc gtaataataa
    10141 aactggaact gccgagtggg gccggattaa ccgccgactc gacgccgtta atgtatcagg
    10201 ggctggaagt cggacagctg actaaactgg atttaaatcc tggtggtaaa gtcactgggg
    10261 aaatgaccgt tgatcccagc gtcgttacgc tgttacggga aaatacccgc atcgaattac
    10321 gcaacccgaa attatccctt agcgatgcca atctcagcgc cctgctgact ggcaaaacct
    10381 tcgagttggt acccggcgat ggcgagccac gcaaagagtt cgttgttgtg ccaagcgaaa
    10441 aagcactgct gcatgaacct gatgttctga cgctgaccct gaccgcgccg gaaagttacg
    10501 gtattgatgc gggtcagccg ctcattcttc acggcgtgca ggtaggccag gttatcgatc
    10561 gtaaactcac cagcaaaggc gtcaccttta ccgtcgccat cgagcctcag catcgagaac
    10621 tggtaaaagg cgatagcaaa tttgtcgtca acagccgtgt cgacgtgaag gtggggctgg
    10681 atggcgttga gtttctcggt gccagcgcct cagaatggat taacggcggg atacgtattc
    10741 tgccgggcga taaaggcgag atgaaagcca gctatccact gtatgccaat ctggaaaaag
    10801 cgctggagaa cagccttagc gatttaccca ccacaaccgt gagtttgagt gcagagacgc
    10861 tgccggatgt gcaggcagga tcggtagtgc tgtaccgtaa atttgaagtt ggtgaagtga
    10921 ttaccgtgcg tccgcgagct aacgcgtttg atatcgatct gcatattaag ccggagtatc
    10981 gcaaccttct gaccagcaat agcgtgttct gggcagaagg cggggcgaaa gttcagctga
    11041 atggtagtgg tctgaccgta caggcatccc cgctctccag agcattaaag ggagccatta
    11101 gcttcgacaa cctcagcggt gccagcgcca gtcagcgtaa aggcgataag cgtattctgt
    11161 atgcttccga aacagcggcc cgtgcggttg gcgggcagat tacgcttcac gctttcgatg
    11221 ccggaaaact ggcggtcggg atgccaattc gctatctcgg tattgatatc gggcaaatcc
    11281 agacgctgga tctgattacc gcacgcaatg aagtacaggc aaaggcggta ctttacccgg
    11341 aatatgtcca gacctttgct cgcggtggta cgcgcttctc agtggtcaca ccgcaaattt
    11401 cggcagctgg cgttgagcat cttgatacta tcctccagcc gtatatcaac gtcgaaccag
    11461 gccggggcaa tcctcgccgc gactttgaat tacaagaggc caccattact gattcgcgtt
    11521 acctggatgg cttaagcatt attgttgaag cgccggaagc cggttcgtta ggtattggta
    11581 cgcctgtgct gttccgtggt ctggaagtag gtacggttac cggaatgacg ctggggacat
    11641 tgtcagatcg cgtgatgatt gcgatgcgca tcagtaaacg ctatcaacac ctggtgcgta
    11701 acaattccgt cttctggttg gcatcgggtt acagtctgga ctttggtctg acgggcggcg
    11761 tagtgaaaac cggcaccttt aaccagttta tccgtggcgg catcgccttc gccacgcctc
    11821 caggtacgcc actggcaccg aaagcccagg aaggcaagca cttcctgttg caggaaagtg
    11881 aaccgaaaga gtggcgtgaa tggggtactg cgcttcccaa ataatgccca ctgctccggc
    11941 gtgcctgcgc cggagcgttt atgctaaact gcgcgcctgt ttttttgcca gtggtatatg
    12001 ctcgtggccc aacacaccgt ttatttcccg gacgcctttc tgacgcaaat gcgcgaagca
    12061 atgccttcga cgctctcttt tgatgatttt cttgccgcct gtcagcgccc gttgcgccgc
    12121 agcattcgcg ttaatacgct gaaaatctcc gttgctgatt tcctgcaatt aaccgctcct
    12181 tatggctgga cgcttacgcc aattccgtgg tgtgaagaag gtttctggat tgaacgcgac
    12241 gatgaagatg cattgccatt gggtagtacc gccgagcatt taagcggcct gttttatatt
    12301 caggaagcca gttcaatgtt gcccgtcgcc gccttgtttg ctgacggtaa tgcaccacag
    12361 caggtgatgg atgtcgctgc tgcgccgggc tccaaaacga cgcaaattgc cgcgcggatg
    12421 aataacgaag gagcgatcct tgccaatgag ttttccgcca gtcgggtaaa agtgttacat
    12481 gccaatatca gccgctgtgg catcagtaat gttgcgctca cacattttga tggccgcgtg
    12541 tttggtgcgg cagtgccaga aatgttcgat gccattttgc tggacgctcc ctgctctggc
    12601 gaaggcgtgg tgcgtaaaga tcccgatgcg ctaaaaaact ggtcaccaga aagcaatcag
    12661 gaaatcgcag ctacacaacg ggagcttatc gacagcgcct ttcatgcatt acgtcctggt
    12721 ggtacgctgg tttactcgac ctgtacctta aaccaggaag aaaacgaagc cgtttgcctg
    12781 tggctgaaag agacttaccc cgacgcagta gagtttttac cacttggcga tctcttccct
    12841 ggtgcaaaca aagcgctgac cgaagaaggc tttttgcatg ttttcccaca aatttacgac
    12901 tgcgaaggct tcttcgttgc tcgtctgcgt aaaactcagg cgatccccgc cttacccgcc
    12961 cccaaataca aagtgggcaa tttcccgttc agcccggtga aagatcgcga agccggacaa
    13021 attcgtcagg cggctgcaag tgttggctta aactgggatg gaaacctgcg actctggcaa
    13081 cgcgacaaag aactgtggtt gttcccggta ggcattgaag ccctgatcgg taaagtccga
    13141 ttttctcgcc tggggattaa acttgccgaa acgcataaca aaggttatcg ctggcagcat
    13201 gaagcggtta ttgcccttgc ctcccccgac aatgtgaacg cttttgaact gacaccgcag
    13261 gaagcggaag agtggtatcg cgggcgcgat gtttacccgc aagccgcgcc agtagcggat
    13321 gatgtattgg ttacttttca gcatcagccg attggtttag ccaaacgaat tggttctcga
    13381 ctgaaaaaca gctacccgcg tgaactggtg cgcgatggga aactttttac cggtaacgcc
    13441 tgacagcgca caaaaaaagc gcactttttg actggcacat tcggctgcct caactaggct
    13501 gaaaaatggt gcgatcgaac tggtcgtacc acaaccggca gctaaatgga gagcacgaag
    13561 atgaaaacca gtgtgcgcat aggcgctttt gaaatcgacg acggcgaatt acacggtgaa
    13621 tcgccgggtg atcgaacgtt aaccattcct tgtaaatctg accccgattt atgtatgcaa
    13681 ctggatgcct gggatgctga aaccagtatc cctgccctgc taaatggcga acactctgtc
    13741 ctttaccgta cccgttacga tcaacaatct gatgcctgga ttatgcgtct tgcctgatcc
    13801 aaaaagaacc cgtcggcatg gcgggttatt tgtcctggtt attcccccgt tgtaaaatct
    13861 ctcctaaact taacggtacg gcaccacact tcggggatga aatgttcgcg ctggtacttt
    13921 ttgtttgcta cctggatggc ggttgtgaag atattgttgt ggatgtctac aacacggaac
    13981 agcagtgtct ttattctatg agcgatcaac ggatccgcca tggcggttgt tttccgattg
    14041 aggattttat agatggtttc tggcgaccag cacaggagta cggtgatttt taattattgc
    14101 aattgcacaa gagtcagttc gcccccaaag acagcgctgg tatcaatata atgcaggttg
    14161 ccaatatcca cgcgatggcg caacggtgta tgaccaaacc agaaatgatc agcacctgta
    14221 attccctgcc ctttttggcg ttcacctaat cgcgagcggc tccacaagac ctgatgcaaa
    14281 tcaacgtcct tttgccattc ataaacatca tctggataat cggcatgagc aataacatgt
    14341 ttgccggtgc gactgtgtac ttcaagaatg aagggcaaat gctgacattt ttccagcgcc
    14401 gttttcgctt gtttctgttg attatctgcc agcgcaataa accagtcgcc gccattcatc
    14461 aaccacaaag acatctgctg ggatgccagc gcatccatcg ccatctgttc atgattgcct
    14521 cttaccgcac gaacccaatg ttgttccagt aactgcaggc aacgtaaact ttgcggccca
    14581 cgatcgataa cgtctcccac tgagataagt aaatctcgcc acggatcaaa accacaatgc
    14641 cataatttgc ggcgcaactg ctcaagacaa ccgtgtatat cgccagaaag ccagatatgt
    14701 cgccattgat gacccgcaat tctctgataa acgggcgcag gctgtttcat caatattttc
    14761 ctcccgcgct aaagatcaca taatcttaac aaaaatgtta aaaaacactg gactcagaca
    14821 gtagagtgtg tgttatggtt gactataaag tcagcgaagg gaatgtttct ggcttttaac
    14881 agataaaaag agaccgaaca cgattcctgt attcggtcca gggaaatggc tcttgggaga
    14941 gagccgtgcg ctaaaagttg gcattaatgc aggcttagtt gccttgccct ttaagaatag
    15001 atgacgacgc caggttttcc agtttgcgtg caaaatggtc aataaaaagc gcggtggtca
    15061 tcagcttaaa tgttaaaaac cgcccgttct ggtgaaagaa ctgaggcggt tttttattgg
    15121 aaatcaaaag gctattttag gtaattaaca gagtttttca gctcgttcta taaacggtgc
    15181 cagactcatt ttttcgccgg gattgttaga atcatcaatc tgaatcaccg aaatggattg
    15241 ggcattggtc ttcccactgg caacttcctt ttgtgcgata tcgtttaaag gatactgcac
    15301 gagggtactc ggattaataa catacaaagc attacccggt cggcaagtca gcatcacctc
    15361 ttcgcgatta aacgcccatt tgtctttacc cacttcaaaa cggctgacgg taatcacctg
    15421 cggtgcagcc agcgccgctg cagaactggt gagtaacaga aatgccagaa tacttttttt
    15481 catcattttc gtaatccatc aaaaaggttc gagagtcgca agcaggctga tgatcaccag
    15541 cactaccgca cctatcgctc attcgagttt agtcatccac acaaaccagg gtgccgcacg
    15601 atcttcatcc tgcctcatgc gcggtacaag aacataacga ttagccagcg caatgaccac
    15661 cataatcatc accaggatgg ctttgagcaa taagagttgg ccccagtaag tcgtaagcgt
    15721 ggggggaaat ccggtaatca acaatgcatt aagcacgccg cttgccagta cgccgatcac
    15781 cgcaaaatgc ccgcaccagg aaaaacgcat cagcgcctga atagcctggt gccgccaacg
    15841 gcctttgatg agttgcatac accagagcac cggcagtaat cccccaaacc acgccgccgc
    15901 acaaatcagg tgaatcgcgt gattagtctg atggattttc gccgtaaccc cttcattcag
    15961 cgtcgcatgc cccactcccg ccagcagaaa aaattgcgcg gtagtgagca taaacagcaa
    16021 acgtggcata ttctgcggtt gcataagggc gacgatcaac gttacgaggg cgagaacaat
    16081 ttgccataac cagacgccac caaactgcgt ttgcaaaaca gcctgccaaa tgtttggcga
    16141 aaatacatct gcccatcccg ttcccatcaa cccgccctga acggcgagca ttgcagtggc
    16201 actgatcaaa ctccagacgg cagcgtgctg ttgtaaacgc agaaaacgct tcgccagcaa
    16261 gcgacgaatc gttaaggggg ccagccaggc accgtacatt gcgaagccaa agaccagcat
    16321 cagcgaggtg aaatggataa atcgcagcgc gatccaggta aacgccagca tgatttattt
    16381 cacgctaaag gtgtagtgtc ctttcgtttt atgcccgtcc accgaaacaa catgccagtc
    16441 aacggtgtag gttccgggtt tcagcgaatc ggccagtgga acaatcagtt gcttttggtc
    16501 ctgctcattc cgcttcgctg gtaatgtttt aatattttcg ttttttggcc ccgtgatttt
    16561 tgcaccactg aatcctgttt caacgccttc cgagaagttt aaagtgattg cctgcggggc
    16621 agctgtcact tgcgcgtttg ccgcaggatg ctgatgcgtt aaatgcgcat gtgcccagac
    16681 ggaaggtgtc accaacgaag tggtcaggat tgccagcgcg tagcgaaggg agcgtgcagt
    16741 tgaagccata ttatctattc ctttttgtaa taactttttt acagagcata accttgtcta
    16801 atgtctgagt cgaggatcat caattccggc ttgccatcca ggctcactct tagtaacttt
    16861 tgcccgcgaa tgatgaggag attaagaatg ctgaagaatc tggctaaact ggatcaaaca
    16921 gaaatggata aagtgaatgt cgatttggcg gcggccgggg tggcatttaa agaacgctac
    16981 aatatgccgg tgatcgctga agcggttgaa cgtgaacagc ctgaacattt gcgcagctgg
    17041 tttcgcgagc ggcttattgc ccaccgtttg gcttcggtca atctgtcacg tttaccttac
    17101 gagcccaaac ttaaataaaa cttatacaga gttacacttt cttacataac gcctgctaaa
    17161 ttatgagtat tttctaaacc gcacccataa tttgtagtca ttttgaaaag gaagtcatta
    17221 tgtcgttctg gaaagttgca gcagcgcaat atgagcctcg taaaacctca ctcaccgaac
    17281 aggtagctca tcatctggag tttgtgaggg ccgccgccag gcaacaatgt cagctgttag
    17341 tttttccatc gctttcttta ctggggtgtg attattcgcg acgtgccctt cctgccccac
    17401 ccgatctctc actgttagac ccgctttgtt atgccgcaac gacctggcga atgaccatca
    17461 tagccggcct tcccgttgaa tataacgatc gctttattcg tggaattgcg gtgttcgcac
    17521 cgtggcgaaa aacgccgggg atctaccatc agagtcatgg tgcatgtctg ggccgtcgtt
    17581 ccagaaccat cacggtggta gatgaacaac cgcaaggtat ggatatggac ccgacctgtt
    17641 cactgtttac gacagggcaa tgtctggggg aacccgacct actggcatct acccgccgct
    17701 tacaattttt ttcacatcag tactccattg ccgtgctgat ggcaaatgcc cgtggtaaca
    17761 gtgcactatg ggatgaacat ggtcgtctca tcgttcgcgc cgatcgtggt tcattattgt
    17821 tagtcggtca gcgttcatca cagggttggc aaggcgatat cattccatta cgctaggctt
    17881 tttcggcctg gagcatgcca tgttgcgcat tatcgataca gaaacctgcg gtttgcaggg
    17941 agggatcgtt gagattgcct ctgttgatgt cattgacgga aaaatcgtca accccatgag
    18001 ccacctggtg cgccccgatc gtcctattag tccacaagcg atggcgattc atcgcatcac
    18061 cgaagccatg gtcgccgata aaccgtggat tgaagatgtg atcccacact attacggtag
    18121 tgaatggtat gtcgcgcata acgccagctt tgaccgccgc gtactgcctg agatgcccgg
    18181 tgagtggatt tgcactatga aactggcccg tcgtttgtgg ccagggatca agtacagcaa
    18241 tatggcgtta tataaaacac gcaagctcaa tgtacagacg ccgccgggcc tgcatcatca
    18301 ccgcgcgttg tatgactgtt atatcaccgc cgcgttgctt atcgatatta tgaacacctc
    18361 cggctggacg gcagaacaga tggccgatat caccggacgt ccgtcgttga tgacgacctt
    18421 cacctttggc aaataccgtg gcaaagcggt ttccgacgtt gccgaacgcg atccgggcta
    18481 tctgcgctgg ttatttaata acctggacag catgagcccg gagctgcgtt taacactgaa
    18541 acattatctg gaaaacactt aatccgcagg cgtagcgggt aatgttccct gcgccagcgc
    18601 gaccagaaaa gcatattcca tcgctacgcc ttcgtacgat ttaaagcgac cagatttacc
    18661 gccatggcct gagtccatgt cggtacagag caataaaaga tggtcatcgg ttttcagctc
    18721 gcgcaattta gcgacccatt ttgccggttc ccaatattgc acctgagaat cgtgcaaacc
    18781 ggtcgttacc agtaaatgcg gataagcctg tgcggtgacg ttgtcatatg ggctgtagct
    18841 tttcatatac tcgtaatatt gcggatcctg cggattcccc cactcttcaa actcaccagt
    18901 ggtaagagga attgattcat caagcattgt tgttacaaca tcaacaaacg gtacctgggc
    18961 gataacgccg tggaataatt cagggcgttc attaatcgca acgcccatca acatgccccc
    19021 cgcactcccg cccatcgcat aacaaagcga aggagagcca tagcccagtt ttaacaatgc
    19081 atcgcaggca tcaagataat cattaaacgt atttttcttc ttcagaaact taccgtcttc
    19141 gtaccattgt tgccccagct caccaccgcc gcgaacatgg acaatggcgt agacaaagcc
    19201 acgatctaac aaactcaagc ggctaaaact gaaatcggca tcaatacttg cgccgtaaga
    19261 accatagcca tacaccagca gcgggttgtg tcctttgcga aaatgtttgc gatgatagac
    19321 cagcgaaacc ggaacttcga cgccatcacg ggcgactatc cacaggtgtt cactgcggta
    19381 attcgccgca tcaaaaccag gaacttccgt ttgttttaat acacgacgct caccggtatc
    19441 catatccagt tcaaacaaag tgtctggtgt ggtcatggaa gaataaccat aacgcaatcg
    19501 cgcggtttca gattctggat tgtaggcaat ccaggtcaca taggccggat catcaaaggc
    19561 aataccaatg acttcccggg tcttgcggtt aatttggcgc aaactggtta acccgcgctg
    19621 acgctcttca accaccagcc agtcggtaaa cagcgtaaac ccttccagca tgatgttttc
    19681 gcgtggcgga attaactctt cccactgttg ctcatcacgc atacgggtac ggtataagcc
    19741 aaagtttttg ccgttgcggt tggaacgcag ataaaaccga tgctggtagt gatcaaggct
    19801 gtattcgtga tctttgcggc gcggcagaaa aacaaacggc tcggcatcgg ccatttccgc
    19861 gtccagcagg cgaacttcac tggtggtggc gctggccaaa tgaatgacta catagtgctt
    19921 cgacgtcgtt ttatgcaggc tgacgtaata ggtatcgtct ttttcttcgt agatcagttt
    19981 atcttgcgat gctggagtac cgatagcgtg acgccagacc tgataaggca gcagcgtcac
    20041 tggatgcttg cgaacatagt agaaagtcca ggagtcattc gcccagacaa agctgggttc
    20101 aacgttatcc agcagttccg ggtaccagtt acctgtttcc agattacgaa aacgaatgcc
    20161 gtactggcgt cgggaaagaa aatcttctgc cagcgccatg atggtgttat cgggcgtaat
    20221 cgccattccg cccatcgaat aaaactcact atgagccgcg cgcttgttgg catcgagcaa
    20281 tatttcccac tcgtcccact cttcactgaa cgccgattga cgctggtaga tagcatattc
    20341 acagcctggt tcataaatat gccgatagcg gtagccattt ttgatgtagg gcgcagaaac
    20401 ttctcgttgc ggaatgcggt cgatgatttc ctttaagatg cgatcctgca aggcttgttg
    20461 tgaggccatc acccgatgac cgtaactatt ttcttgttgc aggtagtcca ggacttctgg
    20521 ctgagaacgc gtatcgtccc gcagccagta gtaattatcg atgcgcgtat cgccatgaag
    20581 cgtcatggcg tggggaatgc gggcggcttt tggtagcatg ttattgttct ttctggttga
    20641 aacatcttat aagggtggca aaactcaccg gggatgcaag cgaaacaggg gagtcattgc
    20701 ttagatgatg acaggtaatg gcgcggatat cgaatgttat gcagacagag aaaatcagcc
    20761 tgttcaaatg gctgtgcgat tctggatagc ccgaaatagt caacttcagg ctatccagag
    20821 agcggaatta ttccgccaaa gtgcgttttt gctgttcgag atcgcgttca atgccgtcac
    20881 gaacatcctg ggggattttc agcgcgtcac ccagtgcatt caggtaactg cgttccataa
    20941 aatggtcaat atcaatagcc gcgcaactca ggaaatagat ttccagcgcc tcttcttcat
    21001 tgcggacccc ggtagccagg cgttgtggat ccagcggttg ttcgattgcc tgctcaatga
    21061 gtacacgccc ctgctcttcc acgccggctt cacgcaattg ctggtcgatt gccgcacgtt
    21121 ctttggcatc aatatgacca tcacttttag cggcaaagac cagcgcaagg atcaaacgtt
    21181 ctgtacgctc atccagcggc gtactttgcg cgccaaactg cggttcgtcc tgatgcgccg
    21241 cgcgaatttt atctttgtat ttattccaca gcaccgtacc cgctaccgct ccgccgccaa
    21301 ccagtaacgc gttggtgcca tatttcgtaa gaagtttacg tgctgattta tttacgacca
    21361 gcagcccagc cagtccgcct aatgcgcctg gcactaacag tttgcccaat ccctgatccg
    21421 cagacgagga ggtagaagaa ctgctttgcc caagcaggga ttgcagttga tttaaccagt
    21481 tagccatatt tgctcctcaa taaccatttt tagtgcctgt agcatagcgg atgaggatgt
    21541 cagaaaatgt atatcgagac aaaagatgcg caaataagtg gggcggtagg ccgttattca
    21601 aagaaattcg cgccaggtaa ggtatttact gtatatcagg tgataagggt attttaggtg
    21661 aaagtagttt gtcgttttga gtggcattgt ttgatgctga ttgcccaatg cgacgctgtt
    21721 gcgtcttctc aggcctacga ttgtggtgcg ttctggggcg gacaaggcac tcgcgccgca
    21781 tccgccaacg gtcaatcgaa ttatcagaag ccattaacgc cgctgatatt ccgccattcc
    21841 cgctttgcag tccacgctga cctggtaatg aatatcggca cttttaccac gcacggttag
    21901 cggtactgac catttatcat ctttaccctg aatgtcctgc aaactgaccc aagccacggg
    21961 atcggcctga ccgacaattt tttgatcatc tgcccagcgc gccacgcgat tttgttgata
    22021 atcacgtttt acgctcgcgg caattccggc agcatccaga ccttcacact ttgggaaagt
    22081 gaccgacttg ctggtttcat tattggcagc gaaaactgat gcgcaggcag aaaccaacaa
    22141 cagccctaaa aacgcccctc tttttttcat gtttttctcc atagcacaat gattcaggag
    22201 aaagcatggt acaaattgtc aggagcgcaa gttgcttcag gccgcgtgtg aggcatcttc
    22261 ctcttcggat tcagcaccga cgacctgtgg ggacggtaat ttgccggttt tcagaatggt
    22321 gctcagcaca tctttttgtt ctgccagcca tagcgaaagg gcttcacgtt gctcgtcttc
    22381 catttgcact ggagaatttg tcagccaggt gtcaagcaga tccgccgtat caagcatttt
    22441 gtcataagca tcggcttcct ttttgctggt aaacgacatt ttctcttcgc cctcacgaat
    22501 gactacgtat ttaacttcaa ccgccatttg cagcctctca taataactgt gattttatac
    22561 agtgtatttc ttttcggttg agaaatcaac accagcaata aagacacacg caaacgtttt
    22621 cgtttatact gcgcgcggaa tttatcaggg gatattcgtt atgacgttat taggcactgc
    22681 gctgcgtccg gcagcaactc gtgtgatgtt attaggctcc ggtgaactgg gtaaagaagt
    22741 ggcaatcgag tgtcagcgtc tcggcgtaga ggtgattgcc gtcgatcgct acgccgacgc
    22801 accagccatg catgtcgcgc atcgctccca tgtcattaat atgcttgatg gtgatgcatt
    22861 acgccgtgtg gttgaactgg aaaaaccaca ttatatcgtg ccggagatcg aagctattgc
    22921 caccgatatg ctgatccaac ttgaagagga aggactgaat gttgtcccct gcgctcgcgc
    22981 aacgaaatta acgatgaatc gcgagggtat ccgtcgcctg gcggcagaag agctgcagct
    23041 gcccacttcc acttatcgtt ttgccgatag cgaaagcctt ttccgcgagg cggttgccgc
    23101 cattggctat ccctgcattg taaaaccggt gatgagctct tccggcaagg ggcagacgtt
    23161 tattcgttct gaagagcaac ttgctcaggc atgggagtac gctcagcaag gcggtcgcgc
    23221 cggagcgggc cgcgtaattg ttgaaggcgt cgttaagttt gacttcgaaa ttaccttgct
    23281 aaccgtcagc gcggtggatg gcgtccattt ctgtgcaccg gtaggtcatc gccaggaaga
    23341 tggcgactac cgtgaatcct ggcaaccaca gcaaatgagc ccgcttgccc ttgaacgtgc
    23401 gcaggagatt gcccgtaaag tggtgctggc gctgggcggt tatgggttgt ttggtgtcga
    23461 gttgtttgtc tgtggcgatg aggtgatttt cagtgaagtc tcccctcgcc cgcatgacac
    23521 cgggatggtg acgctaattt ctcaggatct ctcggagttt gccctgcatg tacgtgcctt
    23581 cctcggactt ccggttggcg ggatccgcca gtatggtcct gcagcttctg ccgtaatcct
    23641 gccacaactg accagtgaga atgtcacgtt tgataatgtg cagaatgccg taggcgcagg
    23701 tttgcagatt cgtttatttg gtaagccggg aattgatggc agccgtcgtc tgggtgttgc
    23761 gctggcggcc gctgagagtg ttgatgaagc gattgagcgc gcgaagcacg ccgctggaca
    23821 ggtaaaagta cagggttaaa cccggcaaaa aaacgctaca aaaatgcccg atacgcgatc
    23881 gggcattttg acttttacag cttagcgcct tctacagctt cacgcgccag cttagtaatg
    23941 cggtcgtaat cgcccgcttc cagcgcatct gccggaacca gccaggaacc accgatgcac
    24001 agcacgcttt tcagcgccag gtagtcacgg tagttagccg gagaaatacc acctgtcggg
    24061 cagaaacgga cctgggagaa cggacccgcg atcgcctgca gggctttcac gccgccgtta
    24121 gcttcagccg ggaagaattt gaactctttc aaaccgtagt ccatacccag catcagttcg
    24181 gaaacagtgc tgatccccgg aatcagagga atagtccctt cggtagcagc tttcagcagc
    24241 ggctcggtca gacccgggct aattgcgaac tgtgcacccg cttcggtgac ttctgccagc
    24301 tgctgtggat tcagcaccgt accggccccc acaatcgctt caggcacttc tttggcgata
    24361 gcacggatag cgtcaactgc acactcggta cgcagagtca cttccagaac gcgcacccca
    24421 ccagcaacca acgcttttgc catcggtacc gcgtgttcca gtttttttac cacgataacc
    24481 ggtacaaccg ggccggtggt gaggattgat tctgcacttg ttttccagtt tttcatcaga
    24541 gttttctctc gcctgattac aaatttgtcg tcttaaaaag tgatacaggt tgcgccctgt
    24601 tcggcaccgg acagtttttc acgcaaggcg ctgaataatt cacgtcctgt tcccacgcgt
    24661 gacgcgctca ggtcaggaat gtgcggttcg cgagcagcca gttccgcttc gtctaccagc
    24721 agcgtcagtt cgcctgtctg tccattcaca cgaatgatgt ccccgtcgcg cacttttgcc
    24781 agcagcccgc catcgtaggc ttctggtgtt acgtggatag ctgacggcac tttacctgaa
    24841 gcgccggaga gtcgtccatc ggtaactaac gcaattttga aacaccggtc caataataca
    24901 ccaagtggcg gcatgagttt atgtaattct ggcattccgt tcgcttttgg cccctgatga
    24961 cggacaacaa cgacacaatc gcggtccagc aaacccgctt caaaggccgg cataacgtca
    25021 tgctggcttt caaaaacaac cgctggcgct tcaatcacct ggttctcaac cggcacggca
    25081 gaggttttca taaccgcacg gcccaggtta ccgcttaaca cttttgtccc accatgatga
    25141 gagaaaggtt gttcgaagga agcgatcaca ttgctgtcga gtgatttttc cgccccttcc
    25201 cgccagtcca gttcaccatt attcagccat ggttcaaggg tataacgaga cagaccaaaa
    25261 cctgccaccg tattgacatc ttcatgcagc aggcctgctt tgagcagttc acgcaccaga
    25321 accggtacgc cacctgccgc ctggaagtgg ttaatatcgg ccggaccgtt cgggtagaga
    25381 cgtgccatca gcggtacaac atcagaaagg tcagagaagt catcccagtt aatctgaata
    25441 ccggccgcgc gcgccatcgc caccaggtgc atggtgtggt tagtggaacc accggtcgcc
    25501 agcagtgcaa cgataccgtt caccaccact ttctcatcga tcatcttacc gatcggcatc
    25561 cattcattac cattaccggt catgcgtgta acctgacgcg cagctgcggc ggtcaaagca
    25621 tcgcgcagcg gagaatccgg atgaacaaaa gaagagcctg gcaactgcat ccccataaac
    25681 tccaccacca tctggttggt gttggcagta ccgtagaaag tacatgttcc cggcgcatgg
    25741 taagacgcgg cttctgactc cagtaaggcc atgcggtcca ctttaccttc ggcataaagc
    25801 tggcgaatac gcactttttc tttatttggc aaaccgcttg ccatcggtcc agacggcaca
    25861 aacaccgcag gcaaatgacc aaacgacagg gctgccatcg tcagacccgg gacaatcttg
    25921 tcgcacaccc cgaggaacag agcaccatca aacatgttat gggacagccc gaccgccgca
    25981 gacatcgcta tcacttcgcg gcttagcagc gacaattcca ttccatcctg cccctgggtg
    26041 acaccatcac acatcgccgg aacaccgccc gcaacctgac caaccgcatt cgcttcatgc
    26101 agggctttac gaatgatttc tggatagtgt tcataaggct ggtgcgcgga gagcatgtcg
    26161 ttataggagg tgatgatggc gatattgtta cgcaacatgc ttttcaaaga ggctttgtct
    26221 tctggctggc aggcagcgaa accgtgtgcc aggttaccgc atgccaactg cgaacgatga
    26281 acggtcgacg ttttcgcttg ttctatccgg gcgagataag cagagcgagt ctcgcgcgaa
    26341 cgttcaatga ttcgatttgt tacgcgtaac atttgtggat tcataaaggc tcctgaaatt
    26401 gagttgtcag agcaggatga ttcacaacgc gtttcattca gaggatttat gagtgaaacg
    26461 cctgtaaccg gagctcatag ggcaaaaacg tttcagtcag tgtaataaaa aaagcctcgt
    26521 gggtgaatcc gcacgaggcc tgaaagtgta aaaattgttc tacaatctgc gcaagatcat
    26581 gttaccggta aaataaccat aaaggataag cgcagatatt actcaaactc attccaggaa
    26641 cgaccatcac gggtaatcat cgccaccgag gcaacgggtc cccaggttcc ggcctgatac
    26701 ggtttcggcg catcattgtc catcgcccac gcctcagtaa tggagtctac ccatttccag
    26761 gcttcttcca cttcgtcgcg acgtacaaac agtgcctgaa taccacgcat ggtttccagc
    26821 agcaaacgtt cataggcatc cgccagatgc gtctgattaa aggtttctga atagctcaga
    26881 tccagcttgg tgatttgcag gttatgtttg tggtcaaggc caggaacttt attcagtacc
    26941 tggatatcca cgccttcatc aggttgcaga cggatagtca gtttattctg cggcagatcc
    27001 tgccacgatt ctttaaacag attcagttca ggtgttttga aatagaccac gacttcagaa
    27061 catttggtcg gcagacgttt accagtacgc aggtagaatg gcacaccggc ccagcgccag
    27121 ttatcaatgt cgacgcggat cgccacgaaa gtttctgtat tgctgctctt gttcgcgccc
    27181 tcttcttcca gatatcccgg cacttttttg ccctgggcga agcccgcagt atattgcccg
    27241 cgtacggttt tttcgcgtac gttggagcgg tcgatgcggc gcagagactt cagtactttc
    27301 actttttcat cgcggatgct gtctgcgctc aggtcagacg gcggagacat cgcaatcatg
    27361 caaagaattt gcagcaggtg gttctggatc atgtcgcgca tctgaccggc tttatcaaaa
    27421 tagccccagc gcccttcgat ccccacttct tctgccacgg taatctcaac atgatcaatg
    27481 gtgcgattgt cccagttatt cacaaacagg gagttagcaa aacgcagcgc caacaggttc
    27541 agcaccgttt ctttaccaag atagtggtcg atacggtaaa cctggcactc ctcgaagtat
    27601 tcgccaacct gatcattgat ttcctgcgag gtcgccagcg acgtccccag cggtttctcc
    27661 atgactacgc gtgccggttt agcattcagt tttgcctcgc caagcccttt gcaaattgcg
    27721 ccaaaagtgc tgggcggcat ggcaaagtag ttaatggtga tacgattttt ttgatccagc
    27781 atcgcgccga gacggctgaa tgcagcagtg tcattgacat cgagattaca aaaatccaga
    27841 cgtgcactca gggtgtccca taaaccttca tcaatggttt ctttcatgaa agtttcgagc
    27901 gcctcgcgga caactttggt atatgccgct ttatcccagt cagcacgccc tacgccgata
    27961 atccgggtgt ccgggttgag ctgaccggct ttttccagtt gatacaggga aggcagcaat
    28021 ttacgacgcg caaggtcgcc tttcgcgccg aaaatgacca ggtcacaggc ctgggctgtt
    28081 tgcgttaccg ccatgtcatt ctccttaagt taactaaccc ggtacttaag ccagggtata
    28141 cttgtaattt tcttacggtg cactgtactg cttttacgag cttgcgaaaa ctgtaaacgc
    28201 ttatccaccc gtgcgattac gggaaaagcg cgcaaagtgc ggcaaaactg ataaaaaaat
    28261 catcgttttc cattgggtaa aaatctgaca ctgatcatgt tatgaaaaaa aataacaact
    28321 tttttatctg cttttgtcat taacacggca cacaggcgta atatctgaca aaactgcatt
    28381 tcgatttctt tcagtgcgga aatcgtcatt acccgtgagt ctctttacat catgaatatg
    28441 ctggaaaaaa tccagtctca gctggaacat ttgagcaaat cagagcgcaa agttgccgag
    28501 gtcattctgg cttcgcccga taacgcgatc cattcgagta ttgctgctat ggcactggaa
    28561 gccaatgtta gcgaaccgac ggtgaatcgt ttctgtcgca gcatggacac gcgcggtttt
    28621 cctgatttta aacttcatct ggcacagagt ctggcgaatg gcactcccta tgttaatcgc
    28681 aatgtcaatg aagatgacag cgttgaatca tacacaggga aaatatttga gtccgcaatg
    28741 gcaacgcttg atcatgtccg tcattcactg gataaatctg ccatcaaccg cgccgtcgac
    28801 ttgctcactc aggcaaaaaa aatcgccttt ttcggattag gctcttcagc cgccgttgcc
    28861 cacgatgcga tgaataagtt ctttcgtttt aatgttccgg tggtgtactc cgatgatatc
    28921 gtgctgcaac gcatgagttg tatgaattgt agcgacggag acgtggtggt gctgatttct
    28981 cacactggaa gaacaaaaaa tctggtcgag ctggcgcagc tggcacgcga aaacgacgcc
    29041 atggtgattg ccctcacctc tgcgggtacc ccgctcgccc gggaagcaac gctggcaatt
    29101 accctcgacg taccggaaga tactgacatt tatatgccca tggtttctcg acttgcacag
    29161 ctgaccgtga tagatgtgct ggcgacagga tttactttgc gacgcggtgc aaaattcaga
    29221 gataacttga agcgggtcaa agaagcgctg aaggaatcgc gttttgataa gcagttactt
    29281 aatttaagtg acgatcgcta aaaacgactg tcactgtcct aatcttatac gacatccgaa
    29341 tgagattaat ttatcgccat cgcggcgtta tttcattcgg atttcatgtt caagcaacac
    29401 ctggttgttt cagtcaacgg agtattacat gtccagaagg cttcgcagaa caaaaatcgt
    29461 taccacgtta ggcccagcaa cagatcgcga taataatctt gaaaaagtta tcgcggcggg
    29521 tgccaacgtt gtacgtatga acttttctca cggctcgcct gaagatcaca aaatgcgcgc
    29581 ggataaagtt cgtgagattg ccgcaaaact ggggcgtcat gtggctattc tgggtgacct
    29641 ccaggggccc aaaatccgtg tatccacctt taaagaaggc aaagttttcc tcaatattgg
    29701 ggataaattc ctgctcgacg ccaacctggg taaaggtgaa ggcgacaaag aaaaagtcgg
    29761 tatcgactac aaaggcctgc ctgctgacgt cgtgcctggt gacatcctgc tgctggacga
    29821 tggtcgcgtc cagttaaaag tactggaagt tcagggcatg aaagtgttca ccgaagtcac
    29881 cgtcggtggt cccctctcca acaataaagg tatcaacaaa cttggcggcg gtttgtcggc
    29941 tgaagcgctg accgaaaaag acaaagcaga cattaagact gcggcgttga ttggcgtaga
    30001 ttacctggct gtctccttcc cacgctgtgg cgaagatctg aactatgccc gtcgcctggc
    30061 acgcgatgca ggatgtgatg cgaaaattgt tgccaaggtt gaacgtgcgg aagccgtttg
    30121 cagccaggat gcaatggatg acatcatcct cgcctctgac gtggtaatgg ttgcacgtgg
    30181 cgacctcggt gtggaaattg gcgacccgga actggtcggc attcagaaag cgttgatccg
    30241 tcgtgcgcgt cagctaaacc gagcggtaat cacggcgacc cagatgatgg agtcaatgat
    30301 tactaacccg atgccgacgc gtgcagaagt catggacgta gcaaacgccg ttctggatgg
    30361 tactgacgct gtgatgctgt ctgcagaaac tgccgctggg cagtatccgt cagaaaccgt
    30421 tgcagccatg gcgcgcgttt gcctgggtgc ggaaaaaatc ccgagcatca acgtttctaa
    30481 acaccgtctg gacgttcagt tcgacaatgt ggaagaagct attgccatgt cagcaatgta
    30541 cgcagctaac cacctgaaag gcgttacggc gatcatcacc atgaccgaat cgggtcgtac
    30601 cgcgctgatg acctcccgta tcagctctgg tctgccaatt ttcgccatgt cgcgccatga
    30661 acgtacgctg aacctgactg ctctctatcg tggcgttacg ccggtgcact ttgatagcgc
    30721 taatgacggc gtagcagctg ccagcgaagc ggttaatctg ctgcgcgata aaggttactt
    30781 gatgtctggt gacctggtga ttgtcaccca gggcgacgtg atgagtaccg tgggttctac
    30841 taataccacg cgtattttaa cggtagagta agtacgttgc cggatgcggc gaaaacgcca
    30901 catccggcct acagttcaat gatagttcaa cagatttcga atattctgaa gcaaacttga
    30961 acttatcatc aggcgaaggc ctctcctcgc gagaggcttt tttatttgat gggataaaga
    31021 tctttgcgct tatacggctg gatttcgccc ggtttgcgag ttttcagcaa ttttagtatc
    31081 caggtgtatt gttctggtcg cggaccaaca aaaatctcga cttcttcatt catccgccgc
    31141 gcaatcgtat gatcatccgc ctctaacaga tcatccatcg gtgggcgcac ctgaatcgtc
    31201 agacgatgcg tcttgccatc ataaatcgga aacagcggta caacgcgcgc acggcacact
    31261 ttcatcaaac gaccaatcgc gggcaacgtc gctttatagg tggcaaagaa atccacaaat
    31321 tcgctgtgct ctgggccatg atcctgatcg ggtaaataat atccccagta cccctgacgt
    31381 accgactgga tgaatggttt aataccgtca tttctcgcat gcagacgacc gccaaagcga
    31441 cgacgcaccg tgttccagac ataatcaaaa accgggttgc cctgattatg gaacatcgct
    31501 gccattttct gcccttgcga ggccatcagc atggcaggaa tatcgacggc ccaaccgtgc
    31561 ggcaccagaa agataacttt ctcgttatta cgccgcatct cttcgatgat ctccagccct
    31621 tgccagtcaa cgcgcggctg aattttctcc ggcccgcgta ttgccaactc agccatcatt
    31681 gccatcgctt gcggcgcggt ggcaaacatc tcatcaacaa tcgcttcgcg ttcagcttca
    31741 ctacgttctg gaaagcagag cgacagattg attaacgcac gacggcgtga gctttttccc
    31801 agtcgtccgg caaaacgtcc cagccgtgcc agaatgggat cacggaactt tggcggcgtt
    31861 aaagcgatac ccgccatcgc tgctacgccc agccatgctc cccagtagcg cgggtggcga
    31921 aaggatttat caaactcagg aatgtattcg ctattatttt ttttcgtttc catgcttttc
    31981 cagtttcgga taaggcaaaa atcaatctgg tgatagtgta gcggcgcaac ttgccccgca
    32041 ccaaataaaa aaagccggta ctgactgcgt accggctgcg aatggatgtt aattaatcaa
    32101 accgtagctg cggcacaatc tctttggcct gcgccaggaa ttcgcgacga tcggagccgg
    32161 tcagcccttc ggtacgcggc agttttgccg tcagcgggtt tacggcctgc tggtttatcc
    32221 atacttcata gtgcagatgc ggcccggttg aacgtccggt attaccggaa agcgcgatac
    32281 ggtcgccacg tttcaccttc tgtcccggtt tcaccagaat cttgcgcaag tgcatataac
    32341 gcgtggtgta gctgcgacca tgacgaatag ccacataata acctgctgcg ccactgcgtt
    32401 tggcaaccac cacttcaccg tcacccactg aaagcactgg cgtaccctgc ggcatggcga
    32461 aatcaacacc tctgtgtggt gcaacgcgac cggtcaccgg attagtacga cgcgggttaa
    32521 agttagacga gatacggaac tgtttcgccg tcgggaatcg caagaatcct ttcgccagac
    32581 cagtaccgtt acgatcgtag aatttgccat cttcagcgcg gatagcgtaa taatctttac
    32641 cttctgaacg caaacgtacg cccagcagct ggctttgctc acgtttacca tcaagcattt
    32701 ctcgagacat taacaccgca aattcatcgc cttttttcag tttgcggaaa tccatttgcc
    32761 actgcatggc tttaatcact gcgctcactt cggcgctggt taaaccggcg tttctggcgc
    32821 tggcaacaaa gcttcccccg acggtacctt tcagcagatt gttaacccac tctccttgct
    32881 gcatttcgct ggtcatttta aaaccgttag cggcagtacg gtcataggtt cgggtttcac
    32941 gacgagacac ttcccaggtg aggcgctgca gttcgccgtc cgcggttaat gtccaggaga
    33001 gttgttgacc gattttcagg ttacgcaatt ctttgtcggc cgcagccagt tgggtgatat
    33061 cacccatatc aataccatac tgattgagaa tgctgcttag cgtatcgcca gtggaaacaa
    33121 catattcatg cacgcccgct tcaccggcga ttttgtcatc cagttcgtcc tggggaatgg
    33181 cttcatcttc ttgtgcagct tgatcaatcg gctcactggc ttcaggtaag agcgaacgaa
    33241 tttcgttctg ttccagctca atggttttga caattggcgt ggcatcacgg tgataaacat
    33301 aaggccgcca gacagcgacg gccagagtaa gaacggtgag cgaccccaac ataacgcggt
    33361 gtggtcgcgg taaattatta aacgccaggg cgacagagcg ggctatctgt tgcacgtaat
    33421 cacttcctca ttaatctcct ttcaggcagc tcgcatactg gttggctaat tgactcagga
    33481 attctgaata gcttgtttta cccagtttga tattcgtccc caggggatcc aacgttccca
    33541 tacgaacgga tgtccctcgt gcgacgctct caacgaccgc tggcctgaac tgtggctcag
    33601 caaaaacgca ggttgctttt tgctcaacca actgtgttct tatttcatgt aaacgctgcg
    33661 cgccaggttg aatctcaggg ttaacggtaa aatgaccaag cggtgtcagt ccaaactgtt
    33721 tttcgaaata gccgtaagca tcgtgaaaaa cgaaataacc tttccccttg agcggcgcga
    33781 gctcgttacc aacctgcgtt tcggttgagg ctaattgtgc ctcaaaatcc ttcaggttgg
    33841 cgtcaagttt ggctcgactt tgcggcataa gttccactaa ttttccatgg attgcaaccg
    33901 ctgtagcccg cgctatctct ggggaaagcc aaagatgcat gttgaaatcg ccgtgatggt
    33961 gatcttcgtc acttttttcc gcgtggtcgt gatcatcatc atcgccgtga atacttttca
    34021 tcagtagcgg tttcacatct tcaagctgcg caatcgttac ctgcttcgct cctggtaatt
    34081 tgcttaccgg tttttgcata aacgcttcca tctccgggcc aacccaaacg actaagtccg
    34141 cgttctgtaa gcgttttaca tccgatgggc gcagtgaata atcatgttct gaagcgccgt
    34201 caggaagtaa aacctctgtt tctgttaccc catcagcaat ggcagaagcg atgaacccaa
    34261 cgggtttaag cgaagcgaca acggcggcat ctgcggcctg tgttgcacct ccccagagag
    34321 cggcggataa tgctgcgaaa agaagcgttt ttttatgtaa cataatgcga ccaatcatcg
    34381 taatgaatat gagaagtgtg atattataac atttcatgac tactgcaaga ctaaaattaa
    34441 catgacaagt ctggtttccc tggaaaatgt ctcggtttct tttggccaac gccgcgtcct
    34501 ctctgatgtg tcgctggaac ttaaacctgg aaaaattttg actttacttg ggccaaatgg
    34561 cgcaggtaag tcgacactgg tacgggtagt gctcgggctg gtaacacccg atgaaggggt
    34621 tatcaagcgc aacggaaaac tgcgcatcgg ctatgtaccg cagaagctgt atctcgacac
    34681 cacgttgcca ctgaccgtaa accgtttttt acgcttacgc cctggtacac ataaagaaga
    34741 tattttgcct gcactgaaac gtgtccatgc cgggcacctg attaacgcac cgatgcaaaa
    34801 gctctctggt ggcgaaacgc agcgtgtact gttagcgcga gcattgttaa atcgcccgca
    34861 attattagtg ctggatgaac ccactcaagg cgtggatgta aatggccagg tggcgttata
    34921 tgaccttatt gaccaactgc gtcgtgaact ggattgtggc gttttaatgg tttctcacga
    34981 tctgcatctg gtgatggcaa aaaccgatga agtgctgtgc ctgaatcacc acatttgttg
    35041 ttccggcaca ccggaagttg tttccctgca tccggagttt atttcaatgt ttggtcctcg
    35101 tggtgctgaa caactgggta tctatcgcca tcatcataac catcgtcacg atttacaggg
    35161 acgaattgtt ttgcgtcggg gaaatgatcg ctcatgattg aattattatt tcccggttgg
    35221 ttagccggga tcatgctcgc ctgtgccgcg ggtccgctgg gttcgtttgt agtctggcgt
    35281 cgtatgtctt atttcggtga tacgctggct catgcctcat tacttggcgt cgcgtttggt
    35341 ttgttgctgg acgtgaatcc attctatgcg gtgattgccg ttacgctgct gctggcgggc
    35401 ggtctggtat ggctggagaa gcgtccacag ctggcgatcg acacgttatt agggattatg
    35461 gcgcacagtg ccctgtcgct gggcctggtg gtcgttagtc tgatgtctaa tattcgtgtt
    35521 gatttgatgg cttacttgtt cggtgatttg ctggcagtga cgccggaaga tctcatctcc
    35581 atcgccattg gcgtggtcat cgtggtggct attttgttct ggcaatggcg caatttgctg
    35641 tcgatgacga ttagcccgga tctggcgttt gttgatggtg tgaaattaca gcgcgtgaaa
    35701 ttgttgttga tgctggtgac ggcattgacg attggtgtag cgatgaaatt tgtcggtgcg
    35761 ttgattatta cttcgttgct gattattcct gcagctactg cgcgtcgctt tgcccgtacg
    35821 ccggaacaga tggctggtgt cgctgttttg gtggggatgg tggcagtgac tggcggttta
    35881 accttttccg cattttacga tacgccggct ggtccgtcgg tggtgctatg tgcggcactg
    35941 ttatttattc tcagtatgat gaaaaagcag gccagctaat ctatcgctga acacatttgt
    36001 cggatgcggc gcgagcgcct tatccgacct acggttcgct atctctggct ggccggataa
    36061 gacgcgaata gcgtcgcatc aggcacactg ccaatttcgg atgcggtgcg agcgaccaat
    36121 ccgacttacg gcatttctgg cggcgttatg ccaaagtgat tccacgcccg cgtcgtcgcc
    36181 atacgcccac gcggtgtacg ctgcaaaaag ccttgctgaa tcaaataagg ttccagcaca
    36241 tcctcaatgg tttcacgttc ttcgccaatg gctgccgcaa ggttatccag acctaccggc
    36301 ccaccaaaga acttatcgat taccgccagc aacaatttgc ggtccatata atcgaaacct
    36361 tcagcatcga cattcaacat atccaacgcc tgagcagcga tatctgccga gatggtgcca
    36421 tcgtgcttca cttcggcgaa atcacgcact cgacgcagca gacggttggc aatgcgcggc
    36481 gtaccgcgag cgcgacgagc aacttccagc gcgccgtcat cactcatctc aagccccata
    36541 aagcgtgcgc tgcgactgac gatatattgc agatccggca cctgataaaa ctccagacgt
    36601 tgcacaatac caaaacggtc gcgcaacggt gatgtcagcg aacctgcgcg cgtggttgca
    36661 ccaatcaggg taaacggcgg caaatcaatt ttaatggagc gtgccgccgg accttcacca
    36721 atcatgatat ccagttggta gtcttccatt gccgggtaca gcacttcttc aacaactggc
    36781 gataaacggt ggatctcatc aataaacagc acgtcatgcg gttcaaggtt agtgagcatc
    36841 gcagccaaat cgcccgcctt ttccagcacc ggaccagaag tcgtgcgtaa attaacgccc
    36901 atttcattgg cgacaatgtt ggcaagcgta gttttaccca accccggagg accaaaaatc
    36961 aacaaatgat cgagggcatc gccgcgcagt ttcgctgctt tgatgaaaat ctccatctgt
    37021 gaacgaacct gcggctgacc aacatactct tccagtaatt tagggcgaat ggcgcgatct
    37081 gctacatctt ccggcaaagt ggtaccggca gaaatcagac ggtctgcttc aatcatcctt
    37141 tacctcataa cgcggcgcgt agggcttcgc gaattaatgt ttcactgctg gcgtcagggc
    37201 gagcgatttt gctcaccatg cggctggctt cttgtggttt atagcccagc gccaccagcg
    37261 cagcaaccgc ttcctgttca gcatcgttgg ttgccgggct ggcaggcgac gtgagcacca
    37321 gatcggcggc tggcgtaaag agatcgccat gcagaccttt aaatcggtct ttcatttcaa
    37381 caatcaagcg ttcggcggtt tttttgccaa tacccggcag tttcaccagc gcccccactt
    37441 cttcacgctc aacggcatta acgaactgct gcgctgacat tccggagagg atcgccagcg
    37501 ccaacttcgg gccgacgccg ttggttttga tcaactcttt gaacaacgtg cgctcttgtt
    37561 tattgttaaa accgtacagc agctgcgcgt cttcacgcac cacaaagtgg gtgaaaacga
    37621 tcgcttcctg tcccgcttca gggagttcat aaaaacaggt catcggcata tgcacttcat
    37681 agcctacgcc gcccacttca attaacacca gcgggggttg tttttcaatg atgatgcctc
    37741 tgagtctgcc tatcacatga cgctcctgcg taatgaatca aagataatgc tgtatgataa
    37801 aaaaatgctg gatagatatc cagcgaagga tgaagaaaac ttgcgaggtg tctcgatgat
    37861 ctgaaaaatg gcacagtata atttattcta caggttattt tggaagcaaa tattttaata
    37921 ttacatattc agtgaaaaaa tgcgtaataa aaatatacat tgcgcctcct gaaaaaataa
    37981 attttttatg ctattacgta tattcgtatc tatttcaatg gaatgacaac gtgaatatta
    38041 attaccctgc tgaatatgaa attggagata tcgcctttac atgtataggt gctgccttat
    38101 ttggtcaaat atcagctgca tcaaattgtt ggagtaatca cgtcgggatc attatcggtc
    38161 ataacggtga agacttcctg gttgcagaaa gccgggttcc cctctcaacc atcactacgc
    38221 tatcccgttt tattaaacgc tctgctaatc aacgctatgc tataaagcga ttagacgctg
    38281 ggctgacaga acaacaaaaa caacgaattg ttgaacaggt tccctcccgg ctacgcaaac
    38341 tttaccacac cggttttaaa tacgaatctt cgcgccagtt ctgttcaaaa tttgtctttg
    38401 atatttataa agaggcgcta tgtattccgg tgggtgaaat agagacgttt ggagaattgt
    38461 taaatagcaa tccgaatgca aaactcactt tctggaaatt ctggttcctg ggttctattc
    38521 cgtgggagcg taaaaccgtt acgccagcca gtttgtggca tcatccgggt ttggtgttga
    38581 ttcatgcgga gggagtggaa acgcctcagc cggaactgac cgaggcggta taacttaacg
    38641 cagtcgccct ctcgccaggt tcagccgcga ttcgctcatc tgcatcgcat tctgactaac
    38701 gtggcagtgg gtgatggcaa tcgccaacgc atcagcggca tccgcctgtg gattagcggg
    38761 cagtttcagc aaggtgcgga ccatatgctg cacctggctt ttttcggcgc taccaatacc
    38821 caccaccgtt tgcttcacct gacgtgcagc atattcaaat accggcaact cctgattcac
    38881 cgccgccaca atcgccacgc cgcgcgcctg gcccagtttc agggctgagt cagcgttctt
    38941 tgccataaag acttgttcaa tggcgaaata atcaggctgg aactgggtga tgatttccgt
    39001 cacgcccgca tagatgagtt tcagacgaga cggtaaatca tccactttgg tgcgtatgca
    39061 tccgctaccc aggtaggaca gttgcctacc tacctggcgg atgacgccgt agccggtcac
    39121 gcgcgaaccc ggatcaatgc cgagaataat agccatcacg cgtctccgtt ttgctgttta
    39181 gcaggcctca tcagagagtc gctgcgacct catcagagat ttcaccgtta tggtaaactt
    39241 cctgcacgtc gtcgcaatct tccagcatat cgatcagacg catcagtttc ggtgcggttt
    39301 ctgcatccat atcagctttg gtagacggga tcatggaaac ttccgcgctg tctgctttca
    39361 gacctgccgc ttccagagcg tcgcgcactt tacccatttc ttcccatgca gtgtagacat
    39421 caatcgcgcc gtcatcatag gtcacaacgt cttcagcacc tgcttccagt gctgcttcca
    39481 tgatggtgtc ttcatcgcct ttctcgaagg agatcacgcc ttttttgctg aacagataag
    39541 ctacggaacc atcagtaccg aggttaccgc cacatttgct gaatgcatga cgcacttcag
    39601 caacggtacg gttgcggttg tcagacagac attcaatcat gattgccgtg ccgccaggac
    39661 cgtaaccttc gtagatgatg gtttccatgt ttgcatcatc atcaccgccc acaccacgtg
    39721 caattgcgcg gttcagtgtg tcgcgggtca tgttgttaga cagtgcttta tcaattgctg
    39781 cacgcagacg cgggttagcg tccggatcgc caccgcccag cttagccgcg gttaccagct
    39841 cacgaatgat tttagtgaag attttaccgc gcttagcatc ctgcgcagct ttacgatgtc
    39901 tggtgttggc ccatttacta tgacctgcca taaaaatatc tccagatagc cctgcctgtt
    39961 caggcagcgt taattacaaa ctgttcaatc gcctgccggt tgctccagga cttagtgagc
    40021 gccgccgcag caggcgcatc aagccacttg taagccagat gttcagtgaa aacgatctgc
    40081 cgctcgtgcg gaagcgcaag acagaaccat gattccgtat tacgcgtcac gcccggcgca
    40141 tagcgatgac gtaaatgtga aaaaatttca aactctaccg tgcgctgaca gtcaattaag
    40201 gtcagttgtt cagcgacaac atcaatggtg acctcttcct ttacttcgcg catggcagct
    40261 tgcggcgcgg tttcaccctc ttccacgctg ccggttaccg actgccagaa atcgggatcg
    40321 tcacgccgct gcaacatcag cacccgtttc gtatcttgtg cgtagatgac cactaagatc
    40381 gaaacgggac gcttatacac tttatccttc acgctgcctc ttcgttgact gccttcgctc
    40441 atcccattca catagttatc tatgctcatg ggagttcact cagttgccgc ctcgatgcaa
    40501 cgcgaatgat ttcgtgtatt tgagtcatat cagttattct cagccttctt cacaacctga
    40561 atgctcagct cagccagtgc agtcgggtta gcaaagctcg gtgcttcagt catcagacac
    40621 gctgccgccg tggttttcgg gaaggcgata acgtcacgga tattgtcggt gccggtcagc
    40681 agcatggtca gacggtcaag accgaatgcc agacctgcgt gcggcggagt accgtatttc
    40741 agagcgtcga gcaggaagcc gaatttctcg cgctgttcct cttcgttgat acccagaata
    40801 ccaaacaccg tctgctgcat atcaccatta tggatacgta ctgaaccacc gcccacttcg
    40861 taaccattga tgaccatatc gtaagcgttc gccaccgcat tttccggtgc agctttcagt
    40921 tctgcagccg tcatatcttt cggtgaggtg aacggatggt gcattgccgt caggccgcct
    40981 tcaccgtcgt cttcaaacat cgggaagtcg ataacccaca gcggtgccca tttgctttcg
    41041 tcggtcagac caaggtcttt acccactttc aggcgcagtg cacccatcgc gtcggcaaca
    41101 attttcttgt tgtcggcacc gaagaaaatc atatcgccat cttgcgcggc agtacgatcc
    41161 aggatgtctt cgatgatttc tgcattaagg aacttcgcta ccgggctgtt gataccttcc
    41221 agacctttcg cgcgttcgtt aactttgatg taagccagac ctttcgcgcc gtagatttta
    41281 acgaagttac cgtattcgtc gatctgctta cgggtcagcg atgcgccgcc cggaacgcgc
    41341 agagccgcta cgcgaccttt cggatcgttc gccggacctg caaatacagc aaactcaaca
    41401 gatttcagca gatcagcaac gtcagtcagt tccatcgggt tacgcagatc cggtttatca
    41461 gaaccataac ggcgttctgc ttccgcaaag gtcattaccg ggaaatcgcc cagatccaca
    41521 cccttcactt ccagccacag atgacgcacc agcgcttcca tcacttcacg cacttgcggc
    41581 gcggtcatga aagaagtttc cacatcgatc tgagtaaatt caggctgacg gtcagcacgc
    41641 aggtcttcgt cacggaagca tttaacgatc tgatagtaac ggtcaaaacc ggacatcatc
    41701 agcagctgtt tgaacaactg cggggattgc ggcagtgcgt agaatttacc tttgtgcaca
    41761 cgagaaggca ccaggtagtc acgcgcgcct tccggcgtgg ctttggtcag catcggagtt
    41821 tcgatgtcga ggaagccgtg gtcatccata aaacggcgca ccaggctggt gattttagcg
    41881 cgggttttca ggcgctgagc catttccgga cgacgcaggt cgaggtagcg gtatttcaga
    41941 cgcgcttctt cggtgttgac gtggttagag tcaagcggca gaacatctgc gcggttgatg
    42001 atagtcagcg aggacgccag cacttcgatt tcgccggtcg ccatatcgcg gttaatattt
    42061 ttttcgtcac gcgcacgtac ggtgcccgtg acctgaatgc agaactcatt acgcagttca
    42121 gaggccagct ttaacgcgtc cgcacgatcc ggatcgaaaa atacctgcac gataccttcg
    42181 cggtcgcgca tatcgatgaa gatcaggcta ccaagatcac gacgacggtt gacccaacca
    42241 cacagagtca cctgctgccc cacgtgggac aaacggagct gtccacaata ttctgtacgc
    42301 atgagatatc ccttaactta gctgccggcg gatgccccct gctgcgcagg tgaccaagtc
    42361 gcagcgttag ctgtatgtca caactgaatg aaaaaaggcg gctattatac tggaaattct
    42421 gccgcaccgt aagagcctgg cccgcgctgg aacgcctcgt taccacttta tatcgggcct
    42481 gaaatcagac tctacgccag tttgctataa aggtgttgcc cgaactcata aaaattaaca
    42541 aaatttgtcg ttccgccatc ggctaatcgc attaaggtgt gaggcacgat tttgtttttt
    42601 caggagtcat tatgcttgaa cttaatgcta aaaccactgc gctggtggtg atcgatttac
    42661 aagaaggcat cttacctttt gccggtggtc cacatactgc cgatgaggta gttaatcgcg
    42721 ccgggaagct ggcggcgaaa tttcgcgcca gcggtcagcc cgtgtttctg gtgcgcgttg
    42781 gctggtctgc cgattacgct gaagcattaa aacagccggt cgatgcgccc tcaccggcta
    42841 aagtgttgcc cgaaaattgg tggcaacatc ctgctgcatt aggtgcaacc gacagcgata
    42901 tcgaaatcat caaacgtcaa tggggtgcgt tttacggtac ggatctggag ttgcaattac
    42961 gccgccgagg tatcgataca atagtgttat gtgggatctc gaccaatatc ggtgttgaat
    43021 ccaccgcccg caatgcctgg gaacttggct ttaatctggt gattgccgaa gacgcctgta
    43081 gtgccgccag cgccgagcag cacaataaca gtatcaacca tatctacccg cgcatcgccc
    43141 gtgtgcgtag cgtggaagag atcctcaacg cgttatgatt tacatcggtc taccgcaatg
    43201 gtcgcatcct aaatgggtgc ggttggggat caccagcctt gaagagtatg cccgccactt
    43261 taactgcgtg gagggcaaca ccacgcttta cgccctgccg aaacccgagg ttgtcctgcg
    43321 ctggcgtgag cagaccacag atgacttccg cttctgtttt aagtttccgg cgaccatttc
    43381 gcatcaggca gcattacggc attgcgatga tttagtgact gaatttttga cccgcatgtc
    43441 accgttggct ccgcgcattg ggcaatactg gctgcaactg cctgccacat tcggcccacg
    43501 ggagctgcct gcgctttggc attttctcga ttctcttcct ggcgaattta attatggcgt
    43561 ggaagtccgc catccacagt ttttcgccaa aggagaagag gaacaaacgc ttaatcgcgg
    43621 tttacatcag cgcggcgtta atcgggtgat tttcatcaga cacgtttaaa tacaccacct
    43681 aaagcgatac aatcgcgcta ttagttttta tggtggtaaa gaatgaaacg tgtaatcagc
    43741 tacatcagat tcagttctga gcgacaagcc aaaggcgact cactacgcag acagtctaag
    43801 atggttaatg actggcttaa ggctcatcct gagtactacc ttgaccaaga tttatctttt
    43861 cgtgatttag gtgtgagtgg attcactggt aagcacttga aaggtggctt aggtgacttt
    43921 ttaaccgcta tcgagaaagg gcttgttaaa gctggtgata cgctcttgat agaatctctt
    43981 gaccgcctct cacgtcagga cgtaggccaa gcaagcgagc tactcagaaa cattcttcgc
    44041 tctggtgtag atgtagtcac cctgtccgat ggtgaacatt acacaaggga atctcttaaa
    44101 gacccgttag ctctgattaa aagcattttg ataatgcagc ggtcgcatga ggaaagtttg
    44161 aggaagtctg agcgtataca agctgcatgg gatgcaaaga aagaagctgc taaagctggt
    44221 atcaaagttt ctaagcgttg tcctgcatgg cttaagctta atgatgaccg taaaagcttc
    44281 tctgtaatac ctgagaaggt agaaactgtg aagaggtgct ttcagctacg gttagagggc
    44341 aaatctatct gggagattgt caggactctt aatgatgaag gtcaccagac acttaaccag
    44401 tacaaagcag gtaagtttgg tcaaacctca gtgcaagacc ttctgaaaaa tcgctctgta
    44461 attggttact tcacacctaa aggctgtgag gagatagctg gttatttccc ccctatcatc
    44521 cctgagactg atttctacaa agtccagcaa ctatcatatt ctcgttatgg aagaaagcct
    44581 gcttcagata aaccattgag tgtgaatctt ttcaaaggtg ttatccgttg ttctgaatgt
    44641 ggtcatgctc tgattatcac aggctttagc gatacaagaa gcggtatata tcgctgtcct
    44701 tctcgaaatg aaaaccgttg caatgccaaa gggctgtcac gtaagcaaac cgatggtaca
    44761 ttacttgccc tgctatcccg tatagaccga tttgatagcg atatgacgaa taccatagac
    44821 acactgaagc tac
//
LOCUS       DAJOSY010000023        43595 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJOSY010000023 DAJOSY010000000
VERSION     DAJOSY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.023"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            123..665
                     /gene="zapC"
                     /locus_tag="OHN27_001422"
     CDS             123..665
                     /gene="zapC"
                     /locus_tag="OHN27_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCQ2285898.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(662..1771)
                     /gene="ycbX"
                     /locus_tag="OHN27_001423"
     CDS             complement(662..1771)
                     /gene="ycbX"
                     /locus_tag="OHN27_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCQ2285899.1"
                     /translation="MATLIRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            2015..4123
                     /gene="rlmKL"
                     /locus_tag="OHN27_001424"
     CDS             2015..4123
                     /gene="rlmKL"
                     /locus_tag="OHN27_001424"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCQ2285900.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYTNRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4135..6042
                     /locus_tag="OHN27_001425"
     CDS             4135..6042
                     /locus_tag="OHN27_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2285901.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6172..7425
                     /gene="pqiA"
                     /locus_tag="OHN27_001426"
     CDS             6172..7425
                     /gene="pqiA"
                     /locus_tag="OHN27_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCQ2285902.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7430..9070
                     /gene="pqiB"
                     /locus_tag="OHN27_001427"
     CDS             7430..9070
                     /gene="pqiB"
                     /locus_tag="OHN27_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCQ2285903.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9067..9630
                     /gene="pqiC"
                     /locus_tag="OHN27_001428"
     CDS             9067..9630
                     /gene="pqiC"
                     /locus_tag="OHN27_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCQ2285904.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9886..10053
                     /gene="rmf"
                     /locus_tag="OHN27_001429"
     CDS             9886..10053
                     /gene="rmf"
                     /locus_tag="OHN27_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCQ2285905.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10123..10641)
                     /gene="fabA"
                     /locus_tag="OHN27_001430"
     CDS             complement(10123..10641)
                     /gene="fabA"
                     /locus_tag="OHN27_001430"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCQ2285906.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10710..12470)
                     /locus_tag="OHN27_001431"
     CDS             complement(10710..12470)
                     /locus_tag="OHN27_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCQ2285907.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPILIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12656..13108
                     /gene="matP"
                     /locus_tag="OHN27_001432"
     CDS             12656..13108
                     /gene="matP"
                     /locus_tag="OHN27_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCQ2285908.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13184..14224)
                     /gene="ompA"
                     /locus_tag="OHN27_001433"
     CDS             complement(13184..14224)
                     /gene="ompA"
                     /locus_tag="OHN27_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCQ2285909.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14581..15090)
                     /gene="sulA"
                     /locus_tag="OHN27_001434"
     CDS             complement(14581..15090)
                     /gene="sulA"
                     /locus_tag="OHN27_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCQ2285910.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15309..15938
                     /gene="sxy"
                     /locus_tag="OHN27_001435"
     CDS             15309..15938
                     /gene="sxy"
                     /locus_tag="OHN27_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCQ2285911.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15901..18063)
                     /gene="yccS"
                     /locus_tag="OHN27_001436"
     CDS             complement(15901..18063)
                     /gene="yccS"
                     /locus_tag="OHN27_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCQ2285912.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDQSQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18073..18519)
                     /locus_tag="OHN27_001437"
     CDS             complement(18073..18519)
                     /locus_tag="OHN27_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCQ2285913.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18642..20696
                     /gene="helD"
                     /locus_tag="OHN27_001438"
     CDS             18642..20696
                     /gene="helD"
                     /locus_tag="OHN27_001438"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCQ2285914.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLSVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20728..21186)
                     /gene="mgsA"
                     /locus_tag="OHN27_001439"
     CDS             complement(20728..21186)
                     /gene="mgsA"
                     /locus_tag="OHN27_001439"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCQ2285915.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21282..21944)
                     /locus_tag="OHN27_001440"
     CDS             complement(21282..21944)
                     /locus_tag="OHN27_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCQ2285916.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22117..22530
                     /locus_tag="OHN27_001441"
     CDS             22117..22530
                     /locus_tag="OHN27_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCQ2285917.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQKGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22575..22892)
                     /gene="hspQ"
                     /locus_tag="OHN27_001442"
     CDS             complement(22575..22892)
                     /gene="hspQ"
                     /locus_tag="OHN27_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCQ2285918.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22950..24140)
                     /gene="rlmI"
                     /locus_tag="OHN27_001443"
     CDS             complement(22950..24140)
                     /gene="rlmI"
                     /locus_tag="OHN27_001443"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCQ2285919.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24235..24513
                     /gene="yccX"
                     /locus_tag="OHN27_001444"
     CDS             24235..24513
                     /gene="yccX"
                     /locus_tag="OHN27_001444"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCQ2285920.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24510..24839)
                     /gene="tusE"
                     /locus_tag="OHN27_001445"
     CDS             complement(24510..24839)
                     /gene="tusE"
                     /locus_tag="OHN27_001445"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCQ2285921.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24930..25589)
                     /gene="yccA"
                     /locus_tag="OHN27_001446"
     CDS             complement(24930..25589)
                     /gene="yccA"
                     /locus_tag="OHN27_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCQ2285922.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25796..25883)
                     /locus_tag="OHN27_001447"
     tRNA            complement(25796..25883)
                     /locus_tag="OHN27_001447"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25847..25849),aa:Ser,seq:tga)
     gene            26310..27428
                     /gene="hyaA"
                     /locus_tag="OHN27_001448"
     CDS             26310..27428
                     /gene="hyaA"
                     /locus_tag="OHN27_001448"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCQ2285923.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            27425..29218
                     /gene="hyaB"
                     /locus_tag="OHN27_001449"
     CDS             27425..29218
                     /gene="hyaB"
                     /locus_tag="OHN27_001449"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCQ2285924.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            29237..29944
                     /gene="hyaC"
                     /locus_tag="OHN27_001450"
     CDS             29237..29944
                     /gene="hyaC"
                     /locus_tag="OHN27_001450"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCQ2285925.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            29941..30528
                     /locus_tag="OHN27_001451"
     CDS             29941..30528
                     /locus_tag="OHN27_001451"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCQ2285926.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            30525..30923
                     /gene="hyaE"
                     /locus_tag="OHN27_001452"
     CDS             30525..30923
                     /gene="hyaE"
                     /locus_tag="OHN27_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCQ2285927.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            30920..31777
                     /locus_tag="OHN27_001453"
     CDS             30920..31777
                     /locus_tag="OHN27_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCQ2285928.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            31911..33455
                     /gene="appC"
                     /locus_tag="OHN27_001454"
     CDS             31911..33455
                     /gene="appC"
                     /locus_tag="OHN27_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCQ2285929.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRGLEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            33467..34603
                     /gene="appB"
                     /locus_tag="OHN27_001455"
     CDS             33467..34603
                     /gene="appB"
                     /locus_tag="OHN27_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCQ2285930.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            34616..34708
                     /gene="cbdX"
                     /locus_tag="OHN27_001456"
     CDS             34616..34708
                     /gene="cbdX"
                     /locus_tag="OHN27_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCQ2285931.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            34788..36086
                     /gene="appA"
                     /locus_tag="OHN27_001457"
     CDS             34788..36086
                     /gene="appA"
                     /locus_tag="OHN27_001457"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCQ2285932.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(36200..38380)
                     /gene="etk"
                     /locus_tag="OHN27_001458"
     CDS             complement(36200..38380)
                     /gene="etk"
                     /locus_tag="OHN27_001458"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCQ2285933.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSDLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(38400..38846)
                     /gene="etp"
                     /locus_tag="OHN27_001459"
                     /gene_synonym="yccY"
     CDS             complement(38400..38846)
                     /gene="etp"
                     /locus_tag="OHN27_001459"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCQ2285934.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(38834..39973)
                     /locus_tag="OHN27_001460"
     CDS             complement(38834..39973)
                     /locus_tag="OHN27_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCQ2285935.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(40019..42115)
                     /locus_tag="OHN27_001461"
     CDS             complement(40019..42115)
                     /locus_tag="OHN27_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCQ2285936.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQMYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(42115..42861)
                     /locus_tag="OHN27_001462"
     CDS             complement(42115..42861)
                     /locus_tag="OHN27_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCQ2285937.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(42858..43502)
                     /gene="gfcB"
                     /locus_tag="OHN27_001463"
     CDS             complement(42858..43502)
                     /gene="gfcB"
                     /locus_tag="OHN27_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCQ2285938.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
BASE COUNT        10650 a        10937 c        11546 g        10462 t
ORIGIN      
        1 ctggttgttt tatattcatc tctgttgctt atttagacat tttgtacttt tattgttgag
       61 gttattaagc gaagcgacaa tggattgtgt tgctgcggtt ttatagggta ggggagaggc
      121 agatgcgaat taaaccagac gataactggc gttggtatta cgatgaagag cacgatcgaa
      181 tgatgctcga tttagccaat ggtatgctat ttcgctcacg ttttgcgcgc aagatgttga
      241 ctccagatgc cttttctccc gcaggctttt gcgttgacga cgccgcgctc tatttctctt
      301 ttgaagaaaa gtgccgcgat tttaatttat ctaaagaaca aaaagccgaa ctggtgctga
      361 atgcactggt agcaattcgc tatctcaaac cgcaaatgcc gaaaagctgg cattttgttt
      421 cccatggtga aatgtgggtg cccatgccgg gcgatgcggc ctgcgtttgg ttgagcgata
      481 ctcacgagca ggtcaatttg ctggtcgttg aatctggcga aaatgccgca ctatgcctgc
      541 tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca gttgggcgac gccattaaaa
      601 tcatgaacga caggctgaaa ccgcaggtta atgttgacag cttcagcctc gaacaggcag
      661 tctaacgcgc caacttaagt gcagtcttcg gtacacagct acagcaaaga atggtgccat
      721 catcgcccat tgctgatttt ttcagcggcg tgacttcgcc ttctaaaagc tgaacacggc
      781 aacttccaca aatgcccgcg cggcaagaat aagggatacg aattccctga ttttctaatt
      841 gctccagcaa cacctgttgg ttatttccac gaaatgcctg tccctgccag tcaatatcta
      901 catttgcgtc cggttgttgc gtgatgttgg cggtatcatc agcggcagct gcgccgtaaa
      961 tttttgccgg agccgttgcc agaatttcca cctcatcgcc aacgcgaatc acgccgctat
     1021 tacgggcaat taaattctga ccaaaatcga catcgccgtt atcctgggca gtgcggaaag
     1081 attgcaatgt ttttaatggt tcgcctgccg gatgtttttg ccctttttct gggctgacgg
     1141 tggtgaaaat acagcggcta caaggtttaa ccacatcaaa caccacatca ccaatgcgaa
     1201 tcactttcca gcgatcttct tcccaggctg acgcgccgga aaccaccaga ttggggcgga
     1261 attgctccat ttttacactg gccggacaac gttgttggag atcacgtaac gaggcttcgt
     1321 tagcaagaag gtaaggatag ccatcagcaa atgacagagg tacagtgttg tggcgtttca
     1381 cgcgccgggt catttgtggc cccacccagc gtaattgcac ttcgcgggag aaaaatccac
     1441 ttagccattt gttgatcgcg tctggcgcaa ttcgcgcggt aaaatgtgtg ccccaaactt
     1501 cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac atatgcgcta ctgccatctg
     1561 gtgcggtgag atgcaagcca tcatgcacag gtgaaggagt aaaccgtacc atctggggaa
     1621 actggcgagc ggtaataaac gtaccgtcag gttccgtgat cataaagatg cgatcgaagg
     1681 ccagaccact gacatctgcc agagcatgtg taagaccaat gccgcgcatc gatttaacag
     1741 gatgaataaa aagccggatt aatgtcgcca cggtgcggtc ctcaaatgaa aataagccct
     1801 caactttatg acatgacgcg cttattagct ataatgcgca acaattttct tagcgcctgt
     1861 cccattaggc tattttattt gtcagtttgg ccctgggtta tgctcgaaat cctcacgtac
     1921 ttgtgtacgc tccggtttct ccgcgctgtc catgtccaga ctgactgcaa caattacgcc
     1981 tactgcggta ggctcttaga gtaaaagtga cgatatgaat tctctgtttg ccagtacggc
     2041 ccgtgggctg gaagagctgt taaaaactga actggaaaac ctgggggccg ttgaatgcca
     2101 ggtggttcag ggtggggtcc atttcaaggg cgacacacgg cttgtttacc agagcctgat
     2161 gtggagccgc ctggcctcgc gtattatgtt gccgctgggc gagtgtaagg tttacagcga
     2221 tttagacctc tatctcggtg ttcaggcgat caactggaca gagatgttta atcctggcgc
     2281 gaccttcgct gtccacttca gtggtttgaa tgacaccatc cgcaacagtc agtacggcgc
     2341 gatgaaagtg aaagacgcga tcgtcgatgc tttcacgcgg aaaaatctgc cgcgtccaaa
     2401 tgttgatcgc gatgcgccgg atatccgcgt taacgtctgg ctgcataaag aaaccgccag
     2461 tatcgctctt gatctgagtg gtgatggttt acatctgcgt ggctatcgcg atcgtgctgg
     2521 tattgcgccg atcaaagaaa ccctggcagc cgctattgtg atgcgatccg gctggcagcc
     2581 aggaacaccg ctgctcgatc cgatgtgtgg ttccggtacg ttgctgattg aagcagcgat
     2641 gctggcgacc gatcgcgcac caggcttgca ccgtgggcgt tggggcttta gcggctgggc
     2701 gcagcatgat gaagctatct ggcaggaagt gaaagcggaa gcgcaaactc gcgcccgtaa
     2761 aggcctggct gagtatagct ctcattttta cggttcggac agcgacgcac gggtgattca
     2821 acgtgcacgc actaacgccc gtcttgcggg gattggtgaa ctgatcacct ttgaggtgaa
     2881 agatgtcgcg caactgacca atccgctgcc gaaagggccg tacggcacag tgttgagcaa
     2941 cccgccatac ggggaacgtc tggacagcga accggcgctg attgcgctgc atagcctgct
     3001 gggtcggatc atgaaaaacc agtttggtgg ctggaatctc tctttgttta gtgcctcgcc
     3061 ggatctgcta agctgcttgc agctgcgtgc agacaaacag tacaaggcga aaaacggccc
     3121 gctggactgc gtacagaaaa attaccacgt tgccgaaagc actccagaca gcaaaccggc
     3181 gatggtagcg gaagactaca ccaaccgcct gcgtaagaac ctcaaaaaat tcgagaagtg
     3241 ggctcgccag gaagggattg aatgttaccg cctgtatgac gccgatctgc cagaatataa
     3301 cgttgccgtt gaccgttatg ccgactgggt ggtggtgcag gagtatgcgc cgccaaaaac
     3361 tattgatgct cacaaagcgc gtcagcgtct gttcgatatt atcgctgcaa ccatttcggt
     3421 actggggatt gcgccaaaca aactggtgct gaaaacccgt gaacgccaga agggcaaaaa
     3481 ccaataccag aaactgggcg agaagggcga gtttcttgaa gttaccgaat ataacgctca
     3541 cttgtgggtg aacctgacgg attatctcga tactggtctg ttcctcgatc accgcatcgc
     3601 ccgtcgtatg ctcggtcaga tgagcaaagg caaagatttc ctcaacctgt tctcttatac
     3661 cggcagcgcc accgtgcatg cgggattagg cggtgcacgc agcaccacca ccgtggatat
     3721 gtcgcgtact tatctggaat gggcagaacg caacctgcgt ctgaatggcc tgaccgggcg
     3781 tgcgcatcgc ctgattcagg ccgattgcct ggcgtggctg cgtgaggcaa atgaacagtt
     3841 cgatctgatc tttatcgatc cgccaacctt ctctaactca aaacgaatgg aagatgcgtt
     3901 tgatgttcag cgcgatcatc tggcgctgat gaaagatttg aaacgtctgc tgcgtgcagg
     3961 tgggacgatc atgttctcga acaacaaacg tggcttccgt atggatctcg acggcctggc
     4021 aaaactggga ctgaaagcac aagaaattac gcaaaaaacg ctctcccagg atttcgcccg
     4081 taaccgccag atccacaact gctggctgat taccgcagcc tgaaaggaat agtaatgtca
     4141 ttaatcagta tgcatggcgc atggctgtcg ttcagcgacg cgccgcttct cgataacgca
     4201 gaactgcata tcgaagataa cgaacgtgtt tgtctggtgg gccgcaacgg cgcaggcaaa
     4261 tcgacgttaa tgaaaatcct caaccgtgaa caagggctgg atgacggtcg cattatttac
     4321 gagcaagatt tgattgtagc gcgtctgcaa caggatccgc cgcgtaacgt tgagggtagc
     4381 gtttatgatt tcgttgccga aggcattgaa gaacaagcgg aatatctgaa acgctatcac
     4441 gatatttcgc gcctggtgat gaacgacccg agcgagaaaa atctcaacga actggcgaag
     4501 gttcaggaac agctggatca ccacaacctg tggcagctgg aaaaccgcat caacgaagtg
     4561 ctggcgcaac tggggttaga tcctaacgtt gcgctgtcgt cgctttccgg cggctggttg
     4621 cgtaaagcgg cattaggacg cgcgctggtg agtaatccgc gcgtgctgtt gcttgatgaa
     4681 ccgacaaacc acctggatat tgaaaccatc gactggctgg aagggttttt gaaaactttc
     4741 aacgggacga ttattttcat ctcccacgac cgttcgttta tccgcaatat ggcgacgcgc
     4801 attgttgatc tcgatcgcgg caagctggtg acctatccag ggaattacga ccagtacctg
     4861 ctggaaaaag aagaagccct gcgcgtggaa gaattacaaa atgccgagtt cgatcgcaaa
     4921 ctggcgcagg aagaggtgtg gatccgccag gggatcaaag cacgccgtac ccgtaatgaa
     4981 ggccgcgtac gcgccctgaa agcgatgcgt cgcgaacgtg gtgaacgtcg cgaagtgatg
     5041 ggtaccgcaa agatgcaggt ggaagaggcc agccgctccg gtaaaatcgt tttcgaaatg
     5101 gaagacgttt gctaccaggt taacggtaag caactggtga aagatttttc tgcccaggtt
     5161 ctacgtggcg acaaaattgc cctgattggt ccgaatgggt gcggcaaaac cacgctgcta
     5221 aaactgatgc tcggtcagct tcaagcggac agcgggcgta ttcacgttgg caccaaactg
     5281 gaagtggctt atttcgatca gcaccgcgcg gaactggatc ccgataaaac ggtgatggat
     5341 aaccttgccg aaggtaagca agaggtgatg gttaacggca agccacgcca cgtattgggc
     5401 tatttgcagg actttctgtt ccatccgaaa cgggcgatga cgccggtacg tgcgctttct
     5461 ggcggtgagc ggaaccgctt gctgctggcg cgtttgttcc tcaaaccaag caacttattg
     5521 attcttgacg aaccgaccaa cgatcttgat gtcgaaacgc tggaactgct ggaagaactg
     5581 atcgacagct atcagggcac ggtattgctg gttagccacg atcgtcagtt tgtcgataac
     5641 accgttacag aatgttggat cttcgaaggc ggcggtaaaa ttggtcgtta tgtcggcggt
     5701 tatcatgatg cccgtggtca gcaagagcag tatgtggcgc tcaaacagcc tgcggtgaaa
     5761 aaaaccgaag aagccgccgc ggcaaaagca gaaactgtaa aacgcagcag tagcaaacta
     5821 agctataaat tgcagcgcga actggagcag ctaccgcaat tgctcgaaga tctggaggcg
     5881 aagctggaag ccctacagac gcaagtggcg gatgcttcct tcttcagtca gccgcatgag
     5941 cagacgcaaa aagtgcttgc tgatatggct gctgcagagc aggagctgga gcaagccttt
     6001 gaacgctggg agtatcttga agcgttaaaa aatggtggct gatcgcaaaa tagtcgattt
     6061 aaacagagac agctgacaac ggtaaaattg cctgatgaac tgggtttatc aggcatgaaa
     6121 accaacactt tgttagtcat ctgtaaaata gcgcatcatt aaggagtacc aatgtgcgaa
     6181 catcatcatg ccgcgaagca catcctgtgc tcgcagtgtg acatgctggt ggcgttaccg
     6241 cgccttgagc atggtcagaa agcggcatgt ccccggtgtg gcacaacgtt aaccgtggcg
     6301 tgggatgccc ctcggcagcg tcccaccgcc tatgcgttgg ctgcactgtt catgctgttg
     6361 ctgtccaact tgtttccttt tgtgaatatg aacgttgcag gagttaccag tgaaattaca
     6421 ttactggaaa ttcccggcgt gcttttttct gaggactacg ccagcctcgg cacctttttc
     6481 ctgttgtttg tgcaactggt tcccgcgttt tgtctgataa ccattctgtt actggtgaat
     6541 cgcgcggaat taccggtccg tttaaaagag caactggcac gggtgctttt tcaactcaaa
     6601 acctggggaa tggcggagat tttcctcgct ggtgtgctgg tcagtttcgt taaactgatg
     6661 gcttacggca gcattggggt aggcagcagc tttctcccct ggtgtttatt ttgtgtcctg
     6721 caactgcgcg cttttcagtg cgttgatcgt cgctggttat gggacgacat cgccccgatg
     6781 ccagaactgc gccagccgct aaaaccaggc gtcacgggga tacgtcaggg gctgcgttcg
     6841 tgctcctgtt gtacggcaat ccttcctgct gatgaacccg tgtgcccgcg ttgtagtacc
     6901 aaagggtacg ttcggcgtag aaacagcctg cagtggacac tcgcgctgct tgtaacgtcc
     6961 atcatgctgt atcttccggc taatattttg cccatcatgg tgacggattt attaggctcg
     7021 aagatgccgt cgacgattct cgctggggtc attctgttat ggagcgaagg ctcttatccc
     7081 gtcgctgcgg tgatctttct ggccagtatt atggtgccaa cgttaaagat gatcgccatc
     7141 gcgtggctgt gttgggatgc caaagggcat ggcaagcgcg acagtgaaag aatgcatttg
     7201 atttatgaag ttgttgagtt tgtaggccgc tggtcgatga ttgacgtttt cgttatcgcg
     7261 gtgctctcgg cgctggtgcg tatgggaggt ttaatgagta tttatccggc aatgggtgca
     7321 ttaatgtttg ctttagtcgt cataatgaca atgttttctg ctatgacgtt tgacccgcgt
     7381 ttgtcgtggg atcgtcaacc tgaatcagag catgaggagt cctgacagta tggaatctaa
     7441 taatggggaa gccaaaatcc agaaagtgaa gaactggtct cccgtgtgga tatttcctat
     7501 cgtcacggcg ctcattgggg cctgggttct tttttatcat tacagccatc agggaccgga
     7561 agtgaccctg atcaccgcga atgcggaagg aattgaaggt ggcaaaacca ccattaaaag
     7621 ccgtagcgtt gacgtcggcg tggttgaaag cgccacactg gctgatgatt tgacgcacgt
     7681 tgaaatcaaa gcgcggctga attccggtat ggaaaaattg ctgcataaag acaccgtctt
     7741 ttgggtggtg aaaccgcaga ttggtcgcga agggattagc ggcctgggaa cgctgctgtc
     7801 tggagtttat atcgaactgc agccaggcgc gaaaggcagc aaaatggata aatacgattt
     7861 gctggactcg ccaccgttgg ccccgcctga tgcgaaaggt atccgtgtga ttctcgatag
     7921 caaaaaagcc gggcagctct cgccaggaga tccggtgctg ttccgtggct atcgggtagg
     7981 ttcggttgaa accagcacct tcgatacaca aaaacgcaat atcagctatc aactgttcat
     8041 caatgcacct tatgaccgac tggtgaccaa caatgttcgc ttctggaaag atagtggcat
     8101 tgcggttgat ctgacgtcag cagggatgcg tgtggagatg ggctcattga caacgctgct
     8161 gagtggcggt gtcagctttg atgtgccgga aggtctggat ttagggcagc cagtggcacc
     8221 gaaaacagct ttcgttttgt atgatgatca gaagagcatt caggattcgt tgtacaccga
     8281 tcacattgat tatctgatgt tctttaaaga ttcggtacgc ggtctgcaac cgggagctcc
     8341 ggtagagttc cggggtattc gcctgggtac cgtaagcaaa gtgccattct ttgcgccgaa
     8401 tatgcgtcag acatttaacg atgattaccg tattccggta ctgattcgta tcgagccaga
     8461 gcggctgaaa atgcagcttg gcgaaaatgc ggatgttgtt gagcaccttg gcgaattgtt
     8521 gaaacgtggt ttacgcggat cgctgaaaac cggaaacctg gtcactggtg cactgtatgt
     8581 tgatctcgat ttctatccaa atacgcctgc aataaccggt attcgtgaat ttaatggtta
     8641 tcagattatc ccgaccgtta gcggcggcct ggcgcaaatc cagcaacgac tgatggaagc
     8701 gttggataag atcaacaaac tgccattgaa tccgatgatt gaacaggcaa ccagtacgct
     8761 ttctgaaagt cagcgcacaa tgaaaaacct gcaaacgacg ctggatagca tgaacaagat
     8821 cctcgctagc cagtcgatgc agcagttgcc gacggatatg cagtcaacgt tgcgtgaatt
     8881 gaatcgcagc atgcagggct tccagcctgg ctccgcagcc tacaacaaga tggtggcgga
     8941 tatgcagcgc cttgatcagg tgttgcgaga actgcaaccg gtgctgaaaa cgctcaatga
     9001 gaagagtaac gcgctggtat ttgaagcgaa ggacaaaaaa gatccagagc cgaagagggc
     9061 gaaacaatga aaaagtggct agtgacgatt gcagcactgt ggctggccgg atgcagctcc
     9121 ggcgaaatta ataaaaacta ttaccagtta cctgtggtgc agagcggtac acaaagtacc
     9181 gccagccagg gcaatcgtct gttatgggta gagcaggtca ctgttcctga ctatctggcg
     9241 gggaatggtg tggtttatca aaccagtgat gtgaagtatg tgattgccaa caacaacttg
     9301 tgggccagcc cgttggatca acagttgcgc aacaccctgg ttgccaacct gagtacgcaa
     9361 ctgcccggct gggtggttgc ctcccagcct ctgggaagcg cccaggacac gctcaatgtt
     9421 accgtaacgg agtttaacgg tcgctatgat ggcaaggtca ttgtcagtgg tgagtggctg
     9481 ttgaaccacc agggacaact gatcaaacgt ccgttccgtc tggaaggagt gcaaactcag
     9541 gatggttacg atgagatggt taaagtgctg gccggtgtct ggagtcagga agccgcttct
     9601 attgcacaag agataaagcg tctaccttaa ttataaagat ttgtaaatat aaccgtctcc
     9661 ggtatgttgc ctgaggcggt ttttttgtct ctaacgtgcg gaaaaatttg ttcctcttca
     9721 cattttttgt acaaccgaca tgcccgtgta gctcacaaat atgacagtgg cgtgaatttt
     9781 gcgcattgac ggcagttatg attcgcggta ttgcttaact gtgattgcac atttagtaat
     9841 cactgttttc ttttccacca gaaaccagta tgagggaaac gaggcatgaa gagacaaaaa
     9901 cgagatcgcc tggaacgggc acatcaacgt ggttatcagg ccggcatcgc cggacgctca
     9961 aaagaaatgt gtccctatca gacgctgaat caaaggtcac aatggctggg aggctggcga
    10021 gaagccatgg cggacagggt agtaatggcc tgattctgtc tctttaaaaa gaaacctccg
    10081 cattgcggag gtttcgcctt ttgatactct gtctgattat aatcagaagg cagacgtatc
    10141 ctggaacaga ccgactttca ggtcgctggc ggtatagatc agacgaccat caaccagcac
    10201 ttcgccatcc gccaggccca taatcagacg acggttaaca atgcgtttaa agtgaatacg
    10261 gtaggtcact tttttcgctg tcggcagtac ctgaccagtg aatttcactt cgccaacgcc
    10321 cagcgcgcgg cctttacctt cgccgcccag ccagccgagg tagaacccta ccagctgcca
    10381 cattgcgtcc aggcccaggc atcccggcat aaccggatcg ccaataaagt ggcatccgaa
    10441 gaaccacaga tccggattga tatccagttc tgcttcaaca taccctttgt cgaagttacc
    10501 acccgtttcg gtcattttga ccacacggtc catcatcagc atgttcggtg ctggcaattg
    10561 cgggccttta gcgccaaaca gttcaccgcg accagaggca agaaggtctt cttttgtata
    10621 ggattcgcgt ttatctacca tgttctctgt aagccttatt ttattgaagc acgcaggata
    10681 gctaacacgt gtacgctgaa caagtccgat cagttcggaa taaaccagtt cagccaacgt
    10741 aatggccatg gaaaacggtg acgtccttcc tgttgcgatg cttgcgcgat acgttcctgg
    10801 atggtttgca tcagcgtcgt ttggccttcg ccatcccaca ccagatttaa taataacggc
    10861 agtgcgtcag tcacatcgtc tactgcccag atggtgaatt tgccttcttc taccgctttc
    10921 accagttcac tgtgaagact taaatggcga acgttagctg tggggataat gacaccttgt
    10981 ttcccggtta actcacgttg ctggcaaata gcaaagaagc cttcgatttt ctcatttaaa
    11041 ccaccgaccg gctgggcgcg accgaactga tcgactgaac ctgtgatagc gatactctga
    11101 ttcaccggca catcggcgag ggcgcttatc agggcgcaga gttcagccat cgaggcacta
    11161 tctccatcaa cttcactgta tgactgctca aatgtcagcg atgctgagaa ggggatctgt
    11221 tgctcaagct gtagttccga catcaggaac gcttgcatga tcatcatccc tttcgcatgg
    11281 atattgccgc caagctccgc tttgcgttcg atgtcggtga attcaccatc gccaatatgc
    11341 acaacgcagc taatgcgaga aggttcgcca aaagcgcgtg gatgacccgg aaattcaatg
    11401 accgaaaggg cgttaatttg cccgatgcgt tcgccttcgg tttcaatcag gatttgctcc
    11461 tgaaggatct catcctgcat acgttcagcg aggaaacctt cgcgccattc acgctgctgc
    11521 agcattaagt ttagctgctc gccggagaag gtgtcgccat cacacaggga ggcgacctct
    11581 ttacactggc ggaggatcca ctgcgggcta agcggaagtg tttcttgttc accggtgtag
    11641 cgtgctgctt cgcggataag tatcggccag gcatccgctc ccggtgcagg taagtgatta
    11701 tgtctggcgg taaatgtcac ccagcgacac cactgggtta ctgactccgc atcgacaatc
    11761 tgcagagtat cttcaaattc gctataaata gcctgctctg aaagctctgg ctccatctcc
    11821 tggaaatcag ccaatgattc gcgttcgcct accagaatga ctttcagctt caatggcatc
    11881 gaaggcacag agacggggag agggcgcgac tcatcaaacg caacccagtc aaaacgctcg
    11941 cggttaacga tatttttcag ccgcatccac agcagaggtt gcgccagcag tgtacgcaaa
    12001 gagatgatga gaataccgcc atttgcttga tgcaccagac caggctgcag ggtaatgtcg
    12061 ccattaaact ggcgcaggca gccaaagagt tgctccgctt ctacccagtc ggcagcgaca
    12121 acttgcgtta aagtcgcaaa attatcatct gcactcactg cgtgacgtaa gcggatggag
    12181 tggccggaaa cctcatagtg accgcccacc agttggcctg catcgctttg tagcgtacgc
    12241 gcggcattgg caataagatt cagatactca gactcttccg gggccttcgc cagcataaaa
    12301 gaggaggatg ctcgcgtatg cagcaactgc tccagcgcaa attgcagccg cggttgagta
    12361 tcactgaata aaggatcgtt ttcgtcaatc aaatgtggct gagcaaatat ttcctgatag
    12421 ctatcggtat caggaaccag gtcacgccat gcaagtttcg taatggtcaa agttgatgtt
    12481 ttttagtctg ttgtcaaagc cgcgattata ccgtaaccgg cactacagca cacgtagaaa
    12541 gcaccgacaa tactcctggc atgggcgtta aagctcacag gatggagatt cttttcttca
    12601 ctggcctaaa aagctgatat tctgtaaaga gttacacggt aacattgaga tcgctatgaa
    12661 atatcaacaa cttgaaaatc ttgaaagcgg ttggaaatgg aagtatctgg ttaagaagca
    12721 tcgcgaaggg gagttaatca cccgttacat agaagccagt gccgcccagg aagccgtcga
    12781 tgtgttgctc tcgctggaaa atgaacccgt gctggtaaat ggctggattg acaagcatat
    12841 gaatccggaa ctggtcaatc gcatgaagca aaccattcgg gcaagacgaa agcgccattt
    12901 taatgcggaa caccagcata cgcgcaaaaa atcgatcgat ctggaattta ttgtctggca
    12961 acgtctggct ggtcttgcgc agcgtcgcgg taaaacgctt tctgaaacga ttgttcagct
    13021 gattgaagat gcggaaaaca aagagaaata cgcgaataaa atgtcttctc tgaagcagga
    13081 tctgcaggca ttgctgggta aggaataact gacgaaagtc agttcaattt actaaaggca
    13141 aaaaaaaccc cgcagcagcg gggtttttct accagacgag aacttaagcc tgcggctgag
    13201 ttacaacgtc tttgatacct ttaacttcga tctctacgcg acgatccgga gccaggcagt
    13261 cgatcagtgc agcacgctgt ttcacgttgt cacaggtgtt gccagtaacc gggttggatt
    13321 cgcccatacc tcgtgcggag atcttgtctg ccgggatacc tttggagatc aggtaatcaa
    13381 caacagactg agcacggcgc tcggacagac cctggttgta agcgtcagaa ccgatgcggt
    13441 cggtgtaacc cagaacaact acggaaccgt ctttcggatc caggttgctc agctggctgt
    13501 acagctgatc cagagcagcc tgaccttccg gtttcagggt tgctttgttg aagttgaaca
    13561 gaacgtcaga cttcagagtg aagtgcttgg tctgtacttc cggtgccgga gctggagccg
    13621 gagcaactac tggagctgct tcgccctgac cgaaacggta ggaaacaccc aggctcagca
    13681 tgccgttgtc cggacgagtg ccgatggtgt gtgcgtcacc gatgttgttg gtccactggt
    13741 attccagacg ggtagcgatt tcaggagtga tcgcgtactc aacaccgcca gcgaagaccg
    13801 gagaaacgcc ggtgtcgtgg tttttaccat aaacgttgga tttagtgtct gcacgccata
    13861 ccataccacc cagacgagtg tagatgtcca ggtcgtcagt gattgggtaa cccagtttag
    13921 cggtcagttg aacgccctga gctttgtatg caccgttttc aacgctgcct ttgtacggca
    13981 tacgacctaa ccagtcgtaa cccatttcaa agccaacata cgggttaacc tggtaaccac
    14041 caaaagcacc agcgcccagt tggttttcat gggtcgggcc attgttgttg atgaaaccag
    14101 tgtcatggta ctgggaccag cccagtttag caccagtgta ccaggtgtta tctttcggag
    14161 cggcctgcgc tacggtagcg aaaccagcca gtgccactgc aatcgcgata gctgtctttt
    14221 tcattttttg cgcctcgtta tcatccaaaa tacgccatga atatctccaa cgagataaca
    14281 cggttaaatc cttcaccggg ggatctgctc aatattaact ctaccgatat cttcggctta
    14341 tgccgagcac ccctggcgat gtaaagtcta caacgtagtt gaaaacttac aagtgtgaac
    14401 tccgtcaggc atatgaaaaa aaagtcttgt ataaggtatg tttaatcttt tttgtcagcg
    14461 acaatttaca gaagagaatc gcggaaaccg cttcagacaa gcctccgcaa ggaaaattag
    14521 tcacgactga aagcattggc tgggcgacaa aaaaagttcc aggattaatc ctaaatttac
    14581 ttaatgatac aaattagagt gaatttttag cccggaaagt tgtctcgtgg cgtgagagga
    14641 tgcgcttacc ggacgcataa taaaccccat agcgttacct tcatttgccg catcaacaag
    14701 ttcagcatgc tcttcttcag tcaaatcatc tgccaaccaa ccgatcacca cactgtaatt
    14761 gcccgtgcgt aaagcgcgaa ccattgactc cacagtgtgg caaggggaga gctggctaat
    14821 ctgcattact ttcgttaagg gtagcccaga tgcctgaacc cattcccgac tcagtttttg
    14881 ttgcggtgtt aaccagagtt gccagcgcga ttgctgaccg agttgctgta acaatggcaa
    14941 cagtagaagt tgcgtcatca tgggctgatc ttcgcgatag acaacttcac tgataagccc
    15001 ggctgtagtg ttttccgtag agacacgcgc aattttactt gctgcggatg agaacgacga
    15061 agaacgatgt gcatagcctg aagtgtacat aattaatcca gcccctgtga gttactgtat
    15121 ggatgtacag tacatccagt gacaacaaag atcaacccta ttttcggaaa gagcctcgca
    15181 aattttgtcg ttggtgacgg gaaaacataa attaatcttg ccccttaaga ataagttgcc
    15241 tattttcgta gttaacggat ccgttaatgt gaatcattct tttatgttat gattttaaaa
    15301 ggaattttat gaaaagcctc tcctataagc ggatctataa atcacaagaa tacctggcaa
    15361 cgttgggcac aattgaatac cgatcattgt ttggcagtta cagcctgacc gttgacgaca
    15421 cggtgtttgc gatggtttct gatggtgagt tgtatcttcg ggcttgtgag caaagtgcac
    15481 agtactgtgt aaaacatccg cctgtctggc tgacatataa aaagtgtggc cgatccgtta
    15541 ccctcaatta ctatcgggtt gatgaaagtc tatggcgaaa tcaactgaag ctggtgcgtc
    15601 tgtcgaaata ttctctcgat gcagcgctga aagagaaaag cacgcgcaat acccgggaaa
    15661 gactgaaaga tttgcccaat atgtcttttc atctggaagc gattctcggg gaggtgggga
    15721 ttaaggatgt acgggcgtta cgtatacttg gggcaaaaat gtgttggttg cgactgcggc
    15781 agcaaaacag tctggtgaca gaaaagattc tgtttatgct tgaaggtgcc attatcggca
    15841 ttcatgaagc tgcgctcccg gtggcacgcc gccaggagct tgcagaatgg gctgactctc
    15901 ttacgccgaa acaggagttt cctgcggaac ttgagtaatc tggcgttgca gacgaccaat
    15961 ctcaggcagt aatgcaatca ataatccaac ttgttgtacg accagaggtt ctttgctgtc
    16021 tgcccgtggt tcaagttgct gcatccgctg tttcaggcta gctaatgcct cattgacgcg
    16081 ttcttcatca gcaggttgat gatgtaacgc gtcatcaaca tagcaaactg cgtcatcaag
    16141 aaacgccaga atttcaggat tagttaactg ctcccggtga gcaccgaggg ctgagatata
    16201 gctggtaaac gtatggttaa ggcacagcaa ccgaaacgcg gcttcgcgaa tttgcggggt
    16261 aacgttcggc tcgctggaca tatttgatac caccgacgcc agctcagcat cacggttgtg
    16321 tgcatcgcgg cgggcaatac gatacgccag acggttatca cgcccctgat ggtattgctc
    16381 cagtatggca tcgagatacc gacagttggc ctctgtggcg cgttcgagca tgcgcggcag
    16441 attgcgaaac tgccagtcag gccagatgta gctcactgcc gcccacgcaa tggcacaacc
    16501 aatcagcgta tcgattacgc gaggtaacgc tacttcaaaa ccttcaccca gtaagttaaa
    16561 acacagtagc accaaaagtg tgatgaacat cgttgcatga gcgtattgca cgttacggaa
    16621 ggcaaaaaag agcacgccgg taataaccag cagcaccagc tgcccttcca gtgatggcac
    16681 aaaccacagc acaggaatgc caatggcgat acctaccagc gtaccaataa tccttaactt
    16741 caggcggtgg cgcgtggcgt tatagtttgg ctggcagaca aacaaacttg tcagcaagat
    16801 ccaatacccg tgatgcattc cggttatctg aatgatggcg tagccgaagc acaacaccag
    16861 cgacattctt accgcatgac ggaagagggc ggattccggc gtgaagtgac ggctaagacg
    16921 cagccagata tcactcaacc cgtgcgggct gtcatcagcg agctcatttt cgtcattatt
    16981 atggggtagt gcctgggcct gttctgattc aattgttgcc agttgggcat caatggcgcg
    17041 taaattgttc agcaaaaatc ccagtgtttt gagtaaatcg gcgggtgcgc cgttatcgcg
    17101 catccgctcc agcgcagcat caatatgcgt aaaagcgcgc tcaaaatgcg gatcatgttg
    17161 ataaggctga cgcaacaaaa tacagcgtga cagttgctgg cacgcctggc cctgcatcga
    17221 catcagccgc tgaaaacgga acagcacgtc gctgtggcga aaatgttcac gcaatgtttg
    17281 atactgaata tgagaagagc tggcacgctc gtgaatatcc tgtgcgacaa agtaataatg
    17341 cagcgtgcga cgcgttcccc gttgaccacg atcgccacgt aagcgggtca gcagcgagag
    17401 tttcgtctga ttcaatgtcg ccatcagcag accgttggcg agagccaaat cgtacagcgg
    17461 tgcctggctt tgatcttcaa tatcaggatc aaacatgcgc gacttgagct caagataacg
    17521 cgccagttgt tcatagcaac gcgccaggtt gtcctgcagc gggcggaccg ggaacagcag
    17581 atgaccaata agtgtcagga cgttgtacca gacggcaccg gccagcagat acatcggctg
    17641 ctgataccag tgctcataca gtgatgttcc caacatagtg taaatggcga tcagcaatgc
    17701 accgaaggca attgttgcat agcgttgacc cagaccgccg agcaaaatga agccgctggt
    17761 agagagcgtt aagccaatcg caaatagcca gggccaggga aacagcaatt ctactgaggc
    17821 cgaggcgata aaaaagcaga acagcgtaat gatgaggtta cgcaaacgtc ccgccagtcg
    17881 gtcatcgaga tcggtcagcg ccgctgccac catccccagc gttagcggaa tcgtcagttt
    17941 tacatcaccc agccaccacg gaaacgctgt ggttccacaa agcgcaataa aaatacgcgc
    18001 gtaatacagc caggcgctgt tccaggtata gcgtttgagc aaaggactta gcataaaggc
    18061 catattagtg gattattcaa aacgacgacg tgcattggct tcacgcgcag cttgcgctgt
    18121 ttctaccgat accacgcgac gaccaaccgg ccatagtgca atagcggcaa ttttaaagtt
    18181 cgcaatgccg acaggaatgc caatgattga aatacattgt gcgatgcccg ttgcaatgtg
    18241 catcaggcat aaccaccagc caaagaaaat cagccagaaa atattcaata ccgtaccgcc
    18301 agtattcagc agcacatttt tgccagccgg gttcagttca tcgacatgaa tagcttcatt
    18361 gccataaggc accagagaca gtttagtgat ctcccagcag gatcgtgtca gcggtaaggt
    18421 aaaaatcagc acaatactga ccagagtcgc caacagccag cccagagtgg tggcaaatcc
    18481 gccaagcaca aagttcagaa tgttcaaaac ggtacgcata aaacctcgct ttactgtggt
    18541 tttcagtaat gggcggcaat tgtaacgttt ttttgggctg gagcacgttt tctctgacgg
    18601 ttacactgat aagaaataat ttcgtgtgga tctacagagt catggaactg aaagcgacaa
    18661 cgcttggaaa acgtctggca cagcaccctt acgatcgggc ggtgatcctc aatgccggga
    18721 ttaaagtctc cggcgatcgc cacgaatacc ttattccttt caatcaatta ctggcgattc
    18781 actgtaagcg cggtctggta tggggcgagc tggaatttgt actgccggac gaaaaagtgg
    18841 tgcgtctgca cggcaccgaa tggggcgaga cgcagcgttt ttaccatcat cttgatgctc
    18901 actggcggcg gtggagtggc gagatgagcg aaattgcgtc tggtgtttta cgccagcaac
    18961 tggatttgat tgccacgcgc actggagaaa ataaatggct gacgcgtgag caaacctctg
    19021 gcgttcagca acaaatccgc caggctttgt cggcgttgcc gttgtcggtt aaccgactgg
    19081 aagaattcga taactgccgt gaggcgtggc gtaaatgtca ggcctggttg aaagatattg
    19141 aaagcgctcg gttgcagcat aaccaggcgt ataccgaagc catgcttacc gagtatgcgg
    19201 attttttccg ccaggtcgag tcttcaccgc tgaatccggc gcaggcccgg gcagtcgtta
    19261 atggcgagca ttctctgtta gtgctggcag gtgcaggaag cggaaaaacg tcggtgctgg
    19321 tggcccgtgc aggctggttg ctggcgcgtg gtgaagcgtc ccctgagcaa attttattgc
    19381 tggcgtttgg tcgcaaagcc gctgaagaga tggacgagcg gattcgcgaa cggctacata
    19441 ccgaagacat taccgcacgc acgtttcatg cgctggcgct gcatattatt cagcagggca
    19501 gcaaaaaagt tccgatagtc agcaaactgg aaaatgatac cgctgcccgt catgaactct
    19561 ttattgctga gtggcgcaag caatgcagcg aaaagaaagc gcaggcgaag ggctggcggc
    19621 aatggctgac ggaagaaatg cagtggtcag tgccagaagg taacttctgg gatgatgaaa
    19681 aattacagcg tcgccttgcc tctcgcctcg atcgctgggt aagtctgatg cggatgcacg
    19741 gtggtgcaca ggcagaaatg attgccagtg cacccgaaga gattcgcgat ctgttcagta
    19801 aacgtatcaa gttgatggcc ccgttattaa aagcctggaa aggtgcgctg aaggcagaaa
    19861 acgctgtcga tttttcgggc cttattcatc aggcgattgt gattctggag aaaggtcgct
    19921 ttatcagccc gtggaagcat attctggttg atgaatttca ggatatctcg ccgcagcggg
    19981 cagcgttgtt agcggcatta cgcaagcaaa acagtcagac gacgttgttc gctgttggtg
    20041 atgactggca ggcgatttac cgattcagcg gtgcgcaaat gtcgctcacc accgctttcc
    20101 atgaaaactt tggtgaaggc gaacgctgtg atttagacac gacttaccgt tttaacagtc
    20161 gtatcggtga ggtggcaaac cggtttattc agcagaaccc aggccagctg aaaaagccgc
    20221 taaacagctt aaccaatgga gacaaaaaag ccgtcacgtt attggatgag agtcaacttg
    20281 acgctttgct ggataagctc tctggttatg ccaaaccgga agagcgcatt ctgatcctgg
    20341 cgcgttacca tcacatgagg cctgccagcc tggaaaaagc ggcaacacgc tggccgaagt
    20401 tgcaaatcga ctttatgacc attcatgcca gcaaagggca acaggcggat tacgtcatca
    20461 tcgttggctt gcaggaggga agtgatggtt ttccggctgc ggcgcgggag tcgattatgg
    20521 aagaggcgct actgccaccg gttgaggatt tcccggacgc tgaagaacgg cggttaatgt
    20581 acgtggcgct gacccgggca cgccatcggg tatgggcact gtttaacaaa gagaatccct
    20641 ctccctttgt ggaaatactg aaaaatctgg atgtgccggt ggcgagaaaa ccgtaagaaa
    20701 caggtggcgt ttgccacctg tgcaatatta cttcagacgg tccgcgagat aacgctgata
    20761 atcggggatc agaatatcga ccgcgtcgtt gaaatgcggc gactggatta tgaagtctgc
    20821 cgttgccacg ttggtggcga ccggaatgtt ccataccgtc gccagacgca gcaaggcttt
    20881 cacgtcagga tcgtgcggca cggcatttag tggatcccag aagaaaatca atacatcaat
    20941 tttcccttct gagatcaatg cgccaacctg ctggtcaccc cccattgggc cactcaacat
    21001 cgcgttgacg ttcatgccgg tcgcgcggga aattaagtta ccggtagtgc ctgttgcata
    21061 cagtacgtgt tgttccagta acggttgatg ccgttccacc cagctcatca gcatttgttt
    21121 gcagtgatcg tgtgccacca gcgcaatatg tttccgcgca ggtaaagtgc gagtcgtcag
    21181 ttccataatg tacatccgta gttaactttc ctacagatta ctgtaagcac ttatcgctgc
    21241 aagataaaga ccgaaaaagc ctgcgcacag gcacaaaaat ctcaggaaga tggttgtttt
    21301 tccgcccact gcaggaaagt atttcgcgtt tgtgggtcag ccagtttaaa ccaatacttc
    21361 agccgttgtt ctgtgagcac ctgagactgc ggtggaatag catccagctc ggaaacaccc
    21421 gaaagcagcg tactgtcatc tgccatcatc gtggcaaatt gcggtagtga agcgcgtttc
    21481 gcggatttgt tatagcgttc aacctcgacc tcataatcaa tcgtttttgc cgttgaggta
    21541 atggcgagaa tatccagctt taccggaatc ggcgtcgcat cgccatccaa caattcaagg
    21601 cgcggcgcgg catcaaaatg gttagcttcc cgctcattct ccaggcgagg caggcgaaaa
    21661 ttgacctggt tgatgagctg ggtattaaaa ctgaccacca gcggtgggga gatatacagc
    21721 cgttcttcac tattggaaag atgaatcgtc ttctcaacgc gaaacactaa ctgatgcggt
    21781 ccattatcca gttcaatgct gtccgcaccg cgcagcagag agctggaaac cttcttgccg
    21841 tcgagtacca gaagatcgac atcggttgaa agccgcagcg tggtggcaaa aacggatacc
    21901 ggcaaacata atgcaatcaa ggtggtcacg atgccggttt tcataggtag ctcctgtcaa
    21961 aagaccgccc gaataataat gtgtcaaaat ttttctttaa tcatacttac taacatatag
    22021 acatatttcc cgcttttgct ctcattcatt cgtattagct gcatggttgg catgtcggct
    22081 ttgtcgtaca ctttgttaaa cagtctggag gaaataatga aagaaaccga tattgcaggc
    22141 attttaacgt ccactcacac cattgcgctg gtgggggcaa gcgacaaacc cgatcgtccc
    22201 agctatcgcg tgatgaaata tctactcgac caggggtatc acgtcattcc ggtttcgcca
    22261 aaagttgccg gcaaaacgct gctgggccag aaggggtatg gcacgctggc agacgttccg
    22321 gaaaaagtcg atatggtcga tgttttccgt aattcagaag cggcgtgggg cgtggcacag
    22381 gaagctattg ccattggcgc gaaaaccctc tggatgcaac tgggggtgat taatgagcaa
    22441 gcggcagtac tggcacggga tgccgggtta aatgtggtga tggatcgctg cccggctatt
    22501 gagatccctc gcctgggcct ggccaaataa aaaatccccg gaaggcaaaa accttccggg
    22561 gatttgttca gggattagtt acgcagacgc ggggcctgga gttgtttgcg gatggtctgc
    22621 gccagctcat ccatggatgg ctgttcagga tgttcatcct gcaattcgct acttagctgg
    22681 gcttcagcca ggtaggtatg aaccggtagg ccgttatcgt cctccatcac tacgtgatac
    22741 cacggagcgg cgcgaagctc gtcattcacc gccagctcat caggcgacgg ttcagagagc
    22801 gaataaaccg ggtcgatatc cacgaccact ccgagataac ctaacaggga atggcggacc
    22861 tgctggccga taccgaattt gctggcaatc atagtcacct cccgggaaat ctttaccttt
    22921 gatataaggg taaaattcca catttcaagt tacatgacgc gacaggcaaa ccctttcaga
    22981 tatagccctt ccggataggt agcgatcacc ggatgatcgg ctgcctgacg gaactgctct
    23041 ataaattgta catcacgacc ggcatcaatt gcggcatccg cgatgatttt ctgaaataaa
    23101 tcgctggtca tcagaccgga acaggagaaa gtcaggagaa taccgccttc attcagcagc
    23161 tgaatcgcca gcatgttgat gtctttataa ccacgacacg cgcccatcaa ctggctttta
    23221 ttctcaacaa acttcggcgg gtccatcacg ataacgtcaa atttttcacc gcgatcgcga
    23281 taagtacgca gcaatttaaa gacatcatca cggacaaact cagccttgct cagatccagt
    23341 ttgttcagct caacgttctg ccgtgcaata tccagcgctt cctgggaggt atcaacgctg
    23401 acaacctggc tgcaaccgcc catcagtgcc gatacggcga aaccaccggt ataggagaaa
    23461 cagttcagca cacgtttatt ttcaacgtag cggcgggtag ccaggcggct atcacgctgg
    23521 tccaggtagt agcccgtttt gtgtccgtgc tgaatatcca ccagcagttt cattccgtgt
    23581 tcttcaatcg gcagcagggc aggtggcaac tcgccggtga cggggccctg ggtcagctcc
    23641 attccttctt ttttacgtac cgcgacgtcg ctgcgatcgt aaatcgaaca ttccgggtac
    23701 agcgtttgca gggcactaat taatgccgcg cgctgatatt ctgccccagc actcagcagt
    23761 tgcagcacca gaaaattacc gaaacgatcg atagtaatac ccggcaggcc atcagattct
    23821 ccggcgatta aacgatagct gtcgaggcca tctttttgcg ccagccagtc acgccatttt
    23881 tgtgcttgtt gcaaacggcg ggaaaaaaaa gcaatgtcga tagactcaga cgggtcaaac
    23941 gtccagacgc gcgcccggat ttgcgaagct ggcgaataag cgccgcgtgc taaccatttt
    24001 ccctgatgat caacaatatc gatggtttca ccgaggctgg ctttaccttc catgcgggca
    24061 acggccccgg aaaagaccca cggatggcga cgaagtaatg atttttcgcg ccctttggct
    24121 aacactaaac gtacactcat aatttacttt tctgtcgatg caaagaaatg ggcgtcattg
    24181 tccggagttt tccatggatt tgcaacgcgt ccattaagga taaggagagc agcaatgtcg
    24241 aaagtctgca taattgcctg ggtttacggg cgagttcagg gcgtaggatt tcgctacacc
    24301 acacagtacg aagcgaaaag actgggttta accgggtacg ccaaaaatct tgacgatggc
    24361 agcgtggaag tggttgcttg cggtgaagaa gggcaggtgg aaaaattaat gcagtggtta
    24421 aagagtggcg gcccgcgttc tgcgcgtgta gaacgggtgc tcagcgagcc gcatcatccc
    24481 tcgggggaat taaccgattt tcgtatccgt taaatacatt ttaccggttt tggcaggcca
    24541 gcaattttgg tggcttgctt agccggacct ttcggaaaca gtcggtataa atagcggctg
    24601 ttaccttttt cttcgccaaa tttattcgcc atcgctttta ccagcatacg aatcgccgga
    24661 gaagtattga attccagata gaaatcacgc acaaaacgca ccacttccca gtgttccggt
    24721 gacagcgaaa tcccttcgtt ctctgcaatc accacagcca gcggctcact ccactggctg
    24781 ctttctttga gatagccttc ggtatccgtt tctatctctt taccttcgaa gatcagcata
    24841 attattacta ccttaatcag actggcggca gtgtaacaaa aaacaaagcc ccgcataaag
    24901 cgaggctatg aaagtgttag cgggtgagat taatcgcggc tagcgaagcc cagaatgctc
    24961 agcaggctga cgaagatgtt gtacagcgaa acatacaggc taaccgtggc acgaatatag
    25021 ttcgtctcac cgccatgaat gatgttgctg gtttcaaaca agatagcgcc agaggagatc
    25081 agaatgaaga ccgcgctgat cgccagatgc agagcaggca gctgcaggaa gatattcgca
    25141 accataccaa tcagcaccac cacaataccc gccatcagca taccgccgag gaacgacata
    25201 tctttgcggg tggtcagcac atatgcagag cagcagaaga acactaacgc cgttccgccc
    25261 agtgccatag cgattacgtc acccattccg gcagacagat aggtgttcag aataggtccg
    25321 aggatataac ccagaaaacc ggtaaaggcg aatgcggaga taatcccggt cggcttattc
    25381 gccgttttat aggtcaggaa catcaaacca tacataccca ccagcgtcag aatcagaccc
    25441 ggagatggca gcatcagcac cgtactggca gttgcggtaa tcgccgaaaa ggccagcgtc
    25501 aggctcagca gaaaataggt attacgcagc accttatggg tgctaagcag tgatgtacgg
    25561 tcatgtgaag aactaacaat acgatccatt gagtcactct ctatgacaga tgtaattaat
    25621 taagcagcat aatgataatg cgtaagggca cccagaagtt ttacccatct ttacgcattt
    25681 gatctggaac aggtttaaca gcggattatc aggtcattaa gcaaatataa cgccctgaga
    25741 atttcgacag gcaaaagaaa aaggggttag catttagcta accccttatc ttatttggcg
    25801 gaagcgcaga gattcgaact ctggaaccct ttcgggtcgc cggttttcaa gaccggtgcc
    25861 ttcaaccgct cggccacact tccggaatga cgcgcactat aaacatcccg atgcggcgtg
    25921 taaaccccta atttgtttgt ttgcctgaaa aacagccaaa agtgcattga tagcgtgaaa
    25981 taacagcaga ttgatcattt catcaccatg aattccttct cttttactcg tttagcaacc
    26041 ggctaaacat ccccaccgcc cggcaaaaag aaaaataggt ccatttttat cgctaaaaga
    26101 taaatccaca cagtttgtat tgttttgtgc aaaagtttca ctacgcttta ttaacaatac
    26161 tttctggcga cgtgtgccag tgcagaagga tgagctttcg ttttcagcat ctcacgtgaa
    26221 gcgatggttt gccttgctac agggacgtcg cttgccgacc ataagcgccc ggtgtcctgc
    26281 cggtgttgct aggagaagag acgtgcgata tgaataacga ggaaacattt taccaggcca
    26341 tgcggcgtca gggcgttacc cggcgcagct ttctcaaata ttgtagtctg gctgccacgt
    26401 cgctgggatt aggcgcggga atggcaccaa agattgcctg ggcgctggag aacaaacccc
    26461 gcattccggt ggtatggatc cacggtctgg aatgcacctg ctgtaccgaa tcttttatcc
    26521 gctccgctca cccactggcg aaggacgtca tcctttccct gatttccctc gattatgacg
    26581 ataccctgat ggctgccgcc ggaacccagg cggaagaagt cttcgaagac atcatcacgc
    26641 aatacaatgg caaatatatc ctcgcagtag aaggtaatcc gccgctgggc gagcagggga
    26701 tgttctgtat cagcagcggt cgaccgttta ttgagaaact caaacgtgcc gctgccggag
    26761 ccagcgcgat tatcgcctgg ggaacctgcg cgtcctgggg ctgcgtgcag gccgcgcggc
    26821 ccaatccgac gcaggcaacg cctatcgaca aagtcatcac cgacaaaccc attatcaaag
    26881 tacctggctg cccgccgatc ccggatgtga tgagcgccat cattacttac atggtgacct
    26941 ttgatcgctt gccagatgtc gacagaatgg gccgtccgct gatgttctat ggtcagcgaa
    27001 tccacgataa atgctatcgc cgcgcccact tcgacgccgg agagttcgtc cagagttggg
    27061 atgatgacgc tgcccgcaaa ggttactgcc tgtacaaaat gggctgcaaa gggcctacca
    27121 cctataacgc ctgttcctcc acacgctgga atgatggcgt ttctttccca atccagtctg
    27181 gtcacggctg cctgggctgt gcggaaaatg gtttctggga tcgcggttcg ttctacagcc
    27241 gcgtggtcga tattccgcaa atgggtactc attccaccgc cgataccgtc ggtttaaccg
    27301 cgcttggcgt ggtggcagcg gctgttggtg tgcacgcagt cgccagcgcc gttgaccagc
    27361 gcagacgtca taaccagcaa cctacagaaa ccgaacatca gccaggcaat gaggataaac
    27421 aggcatgagc actcagtacg aaactcaggg atacaccatc aataatgccg gacgccgcct
    27481 ggtggtcgac ccgattacgc gcatcgaagg ccacatgcgc tgcgaagtga atattaacga
    27541 tcagaatgtg atcaccaatg ccgtctcctg cggcaccatg tttcgcgggc tggagatcat
    27601 cctacaaggg cgcgacccgc gcgatgcgtg ggcgttcgtt gaacgtatct gcggcgtctg
    27661 tactggcgta cacgccctgg cttcggttta cgccatcgaa gatgctatcg gtattaaagt
    27721 gccggacaac gccaatatca tccgcaacat tatgctggca acgctctggt gccacgatca
    27781 tctggtgcac ttctatcagc ttgccgggat ggactggatc gatgtgttag atgcgctgaa
    27841 agccgacccg cggaaaacct ccgaactggc gcaaagtctc tcctcttggc cgaaatcatc
    27901 ccctggctat ttcttcgacg tacaaaaccg cctgaaaaaa tttgttgaag gcgggcagtt
    27961 ggggatcttc cgcaatggct actgggggca cccgcagtac aaactgccgc cagaagctaa
    28021 cctgatgggc tttgcccact atctcgaagc tctcgatttc cagcgtgaaa ttgtcaaaat
    28081 ccacgcggtc tttggcggta aaaacccgca tccaaactgg attgtcggcg ggatgccttg
    28141 cgccatcaac attgacgaaa gcggcgcggt cggggcagtc aatatggaac gcctgaacct
    28201 ggtgcagtca attatcaccc gcacggcgga cttcattaac aacgtgatga tccccgacgc
    28261 cttagccatc ggtcagttca acaaaccgtg gagcgaaatc ggcactggtc tttctgataa
    28321 atgcgttctc agctacggcg cattcccgga tattgccaac gactttggcg agaaaagtct
    28381 gctgatgcct ggcggcgcgg tgattaacgg cgacttcaac aatgtgctgc cagtggattt
    28441 ggttgatccg cagcaggtgc aggagtttgt cgaccacgcc tggtatcgat atcccaacga
    28501 tcaggtcggg cgtcatccgt tcgatggcat caccgacccg tggtacaacc ccggcgatgt
    28561 caaaggcagc gataccaaca ttcagcagct gaatgaacag gaacgctact cgtggatcaa
    28621 agcgccacgc tggcgcggta acgcgatgga agtggggccg ctggcgcgca cgttaatcgc
    28681 ttatcacaaa ggcgatgctg cgaccgttga gtcggtcgat cgcatgatgt cggcgttgaa
    28741 cctgccgctt tccggtatcc agtcaacgtt aggccgcatt ttgtgccgcg cgcacgaagc
    28801 gcagtgggcc gcaggtaagt tgcagtattt cttcgacaag ctgatgacca acctgaaaaa
    28861 cggcaatctc gccactgctt ccacggaaaa atgggaacct gcaacctggc cgacagagtg
    28921 ccgtggtgtc ggttttaccg aagcgccgcg cggggcgtta ggccactggg ccgccattcg
    28981 cgatggcaag attgatctct accagtgcgt ggtgccgacc acctggaacg ccagcccgcg
    29041 cgatcccaaa gggcagattg gcgcttatga agcggcgctg atgaacacca aaatggcgat
    29101 ccccgagcaa ccgctggaga tcctgcgtac tctgcacagc tttgacccgt gcctcgcctg
    29161 ttcaacacac gtgctgggcg acgacggtag cgagctgatc tccgtgcagg tgcgttaaca
    29221 gcgaaggaga atcatcatgc aacagaaaag cgacaacgtt gtcagccact atgtctttga
    29281 agcgccagtg cgcatctggc actggttgac ggtgttatgc atggcggtgt tgatggtcac
    29341 cggatacttt atcggcaagc cgctaccttc cgtcagcggc gaggcgacgt atctgttcta
    29401 tatgggctac atcaggttaa ttcacttcag cgccgggatg gtttttaccg tggttttgct
    29461 gatgcggatc tactgggctt ttgttggcaa tcgatactcc cgcgagctgt ttatcgtgcc
    29521 ggtatggcgt aaaagctggt ggcagggcgt gtggtatgaa atccgctggt atctgtttct
    29581 ggcaaaacgt ccgagtgccg atataggcca taatcccatc gcccaggcgg cgatgttcgg
    29641 ctattttctg atgtcggtct ttatgatcat cactggtttt gcgctgtaca gcgaacacag
    29701 ccagtacgct atttttgcgc cgttccgtta tgtggtggaa tttttctact ggacgggtgg
    29761 caactcaatg gacattcaca gctggcatcg gctggggatg tggctgattg gcgcgtttgt
    29821 gatcggtcat gtctacatgg cgctgcgtga agacatcatg tccgacgaca cggtgatctc
    29881 caccatggtc aacggctacc gtagccacaa atttggcaaa ataagtaaca aggagcgttc
    29941 atgagcgagc aacgcgtggt ggtcatgggg ctgggcaacc tgctgtgggc cgatgaaggc
    30001 ttcggcgtgc gggtggcgga acggctgtat gcccattacc actggcccga gtatgtggag
    30061 attgtcgatg gcggtactca gggactgaac ttgctggggt atgtcgaaag cgccagccat
    30121 ctgttgattc tcgatgccat tgactacggg ctggaacctg gaacgctgcg aacctatgcc
    30181 ggagaacgca ttccggctta tctcagcgcg aagaaaatga gcctgcatca gaacagtttc
    30241 tccgaagtgt tggcgctggc ggatatccgc ggacatctgc cagcacatat tgccctcgtc
    30301 ggtctgcaac ccgcaatgct cgacgactac ggtggtagcc tgagcgaact ggcacgggag
    30361 caactgcccg ctgcggaaca ggcggcgctg gcgcagcttg ctgcgtgggg aattgtgccg
    30421 caaccggcta atgaatcgcg ctgtctcaat tatgactgtc tgtcgatgga aaattacgaa
    30481 ggcgttcgct tgcgccagta ccggatgaca caggaggagc agggatgagc aacgacacgc
    30541 catttgatgc gttgtggcaa cgaatgctgg cgcgcggctg gacgccagtc agtgaatccc
    30601 gtcttgacga ctggcttacg caagcgccag acggcgtggt gttattaagc agtgacccga
    30661 aacgcacgcc agaggtcagc gataatccgg taatgattgg cgaattactg cgcgagtttc
    30721 ccgactatac atggcaggtg gcgattgctg accttgagca gagcgaagcc atcggcgatc
    30781 gctttggcgt ctttcgcttt cctgccactt tagtgtttac cggcggaaac tatcgcggcg
    30841 tgctgaatgg tattcacccg tgggcggaac tgataaacct gatgcgcggg cttgtcgaac
    30901 cgcagcagga gcgtgcctca tgagcgaaac ttttttccat ctgctggggc caggaacgca
    30961 accgaacgat gacagtttca gcatgaatcc actgccgatc acctgtcagg tgaatgatga
    31021 accgagtatg gcggccctgg agcaatgtgc tcacagcccg caggtgattg cgctgttaaa
    31081 cgagttacaa catcaactaa gcgaacgcca accgccgttg ggcgaggtgc tggcagtcga
    31141 tctgttaaat ctcaacgccg acgatcgtca ctttatcaat acgcttctcg gggaagggga
    31201 agtgtcagtg cgcattcagc aggctgacga cagtgaaagt gaaatacagg aggcgatctt
    31261 ctgcggatta tggcgggtgc gcagacgtcg cggcgaaaag ttgctggagg acaaactgga
    31321 ggctggctgc gcgccgctgg cgttgtggca ggcggcaacg caaaatctct tgccgacaga
    31381 ttcgctgtta ccgccgccca ttgatggcct gatgaatggc ctaccgttgg cgcatgagtt
    31441 actggcacat gtacgtaacc ccgacgcgca gccgcacagc attaatctga cgcaattacc
    31501 catcagcgag gctgatcggc tttttctctc acgtctctgt gggccgggaa atattcagat
    31561 tcgtaccatt ggctatggcg agagctatat caacgccacg gggttacgcc atgtctggca
    31621 tttacgctgt acggacacct taaaaggccc gttactggaa agttatgaaa tctgcccaat
    31681 accggaagtg gtgctggcag cgccagaaga tttggtcgac tctgcgcagc ggcttagcga
    31741 ggtatgtcag tggctggcgg aagctgcacc gacgtaaaaa gacggtaagt atcgctttca
    31801 gtcttatgaa tatcgcaatc ggcgaatacc tctggtcgta gagtttcagg ataaagaggg
    31861 agatctacca ttatcgggtt atttttctct cttcgcctac aggagtgcgc atgtgggatg
    31921 tcattgattt atcgcgctgg cagtttgctc tgaccgcgct gtatcacttt ttatttgtac
    31981 cccttaccct ggggctgatt tttttgctgg ctatcatgga aaccatttac gtggtcaccg
    32041 gcaaaacaat ctaccgcgat atgacgcgct tctggggtaa gctcttcggt atcaattttg
    32101 ctcttggcgt ggctaccggc ctgaccatgg agtttcagtt tggtactaac tggtcattct
    32161 attccaacta tgtgggcgat atttttggcg caccgctggc gatggaagca ttaatggcct
    32221 tcttcctcga atccaccttt gtcgggctgt tcttcttcgg ctggcaacgg ctgaataaat
    32281 accagcacct gctggtgacg tggctggtgg cgttcggttc aaatctctct gcgttgtgga
    32341 tattgaatgc caacggttgg atgcaatacc cgaccggtgc gcattttgat atcgacaccc
    32401 tgcgtatgga gatgaccagc ttcagcgagc tggtctttaa tccggtcagc caggtgaaat
    32461 ttgtgcacac cgtaatggcg ggctacgtga ccggggccat gtttattatg gcgatcagcg
    32521 cctggtattt actgcgcgga cgggagcgcg atgtcgcatt acgctcgttt gccatcggtt
    32581 ccgtcttcgg tactctggcg attatcggta ccctgcaact cggagacagt tctgcgtatg
    32641 aagtcgcgca agtacaaccg gtaaaactgg cggcgatgga aggggagtgg caaacggaac
    32701 ctgcacctgc accgttccat gtggttgcct ggccggaaca ggatcaagag cgtaacgcct
    32761 ttgccctcaa aattcccgcg ctgctaggga tcctcgccac tcactcatta gataaacccg
    32821 tgccgggtct gaagaatttg atggctgaaa cctacccacg cttgcaacgc ggacgtatgg
    32881 cctggctgtt aatgcaggaa atatcgcaag gcaatcgtga gccgcatgtg ttgcaggcat
    32941 tccggggact ggaaggtgac ctgggctacg gcatgttgct ctcccgctat gcgccggata
    33001 tgaatcatgt cacagccgca cagtaccagg cggcgatgcg tggcgcgata cctcaggttg
    33061 cgccggtatt ctggagtttc cgcatcatgg tgggctgtgg ttccctgctg ctactggtga
    33121 tgctgattgc gcttgtccag acgctgcgtg gcaaaatcga ccagcatcgc tgggtgctga
    33181 aaatggcgct ctggagtttg ccgttgccgt ggattgcgat tgaagccggg tggtttatga
    33241 ccgagtttgg tcgtcagccg tgggcgatac aggacatctt accgacatac tccgcgcact
    33301 ccgctttaac cacaggacaa ctggctttct cactgatcat gatcgtaggg ctttacaccc
    33361 tgttcttaat cgccgaagtc tacctgatgc agaaatatgc ccgtctgggg ccgagcgcga
    33421 tgcagagtga acaaccgacg cagcaacagg ggtaaaggag aaaatcatgt ttgattatga
    33481 aacattgcgc ttcatctggt ggctgctgat tggcgtgatc ctggtggtct ttatgatctc
    33541 cgacggattt gacatgggga tcggctgtct gctgccgctg gtggcgcgta atgatgatga
    33601 acgccggata gtgataaaca gcgttggtgc acactgggaa ggcaaccagg tctggttgat
    33661 cctcgctggt ggggcattat ttgccgcctg gcccagagtg tatgcagcgg cgttttccgg
    33721 cttttatgtg gcgatgatcc tggtgctgtg ctcactgttc ttccgcccgc tggcctttga
    33781 ttatcgcgga aaaatcgccg atgcacgctg gcgtaaaatg tgggacgccg gtctggtcat
    33841 cggcagtctg gtgccgccgg tagtcttcgg tatcgccttc ggcaacttgt tgctcggcgt
    33901 gccgtttgcc ttcacaccgc aattacgcgt ggagtatctc ggcagcttct ggcaactgct
    33961 gacgccattc cctttattgt gcggattgct cagccttggg atggtgattt tgcaaggtgg
    34021 cgtctggtta caactgaaaa ctgttggtgt gattcatctg cgttcacagc tggccaccaa
    34081 acgcgctgca ctgttggtga tgctgtgctt tttgctggcg ggttactggc tgtgggtcgg
    34141 tattgatggc tttgtactgc tcgcccagga tgctaacggt ccttccaatc cgttaatgaa
    34201 actggtggca gtgctacctg gtgcctggat gaataatttc gtcgaatcgc ccgttttgtg
    34261 gatcttcccg ctgctggggt tcttctgccc attgctgacg gtgatggcga tttatcgtgg
    34321 tcgcccgggt tggggatttt taatggcgtc attgatgcaa tttggcgtga ttttcacggc
    34381 aggcatcacg ctgttcccct ttgtcatgcc gtcaagcgtg agtccaatct ccagcctgac
    34441 gttgtgggac agcacttcca gtcagctgac gctgagcatt atgttggtaa tcgtgctgat
    34501 atttttgccc attgtgttgc tctacactct ctggagctac tacaaaatgt ggggacgcat
    34561 gacaacagaa actctccgcc gtaacgaaaa cgagttgtac taaggagcag gaacaatgtg
    34621 gtatttactt tggttcgtcg gcattttgtt gatgtgttcg ctctccaccc ttgtgttggt
    34681 atggctggac ccgcgtctga aaagttaacg aacgtaggcc tgatgcggcg cattagcatc
    34741 gcatcaggca atcaataatg tcagatatga aaagcggaaa catatcgatg aaagcgatct
    34801 taatcccatt tttatctctt ctgattccgt taaccccgca atctgcattc gctcagagtg
    34861 agccggagct gaagctggaa agtgtggtga ttgtcagtcg tcatggtgtg cgtgctccaa
    34921 ccaaggccac gcaactgatg caggatgtca ccccagacgc atggccaacc tggccggtaa
    34981 aactgggttg gctgacaccg cgcggtggtg agctaatcgc ctatctcgga cattaccaac
    35041 gccagcgtct ggtagccgac ggattgctgg cgaaaaaggg ctgcccgcag tctggtcagg
    35101 tcgcgattat tgctgatgtc gacgagcgta cccgtaaaac aggcgaagcc ttcgccgccg
    35161 ggctggcacc tgactgtgca ataaccgtac atacccaggc agatacgtcc agtcccgatc
    35221 cgttatttaa tcctctaaaa actggcgttt gccaactgga taacgcgaac gtgactgacg
    35281 cgatcctcag cagggcagga gggtcaattg ctgactttac cgggcatcgg caaacggcgt
    35341 ttcgcgaact ggaacgggtg cttaattttc cgcaatcaaa cttgtgcctt aaacgtgaga
    35401 aacaggacga aagctgttca ttaacgcagg cattaccatc ggaactcaag gtgagcgccg
    35461 acaatgtctc attaaccggt gcggtaagcc tcgcatcaat gctgacggag atatttctcc
    35521 tgcaacaagc acagggaatg ccggagccgg ggtggggaag gatcaccgat tcacaccagt
    35581 ggaacacctt gctaagtttg cataacgcgc aattttattt gctacaacgc acgccagagg
    35641 ttgcccgcag ccgcgccacc ccgttattag atttgatcaa gacagcgttg acgccccatc
    35701 caccgcaaaa acaggcgtat ggtgtgacat tacccacttc agtgctgttt atcgccggac
    35761 acgatactaa tctggcaaat ctcggcggcg cactggagct caactggacg cttcccggtc
    35821 agccggataa cacgccgcca ggtggtgaac tggtgtttga acgctggcgt cggctaagcg
    35881 ataacagcca gtggattcag gtttcgctgg tcttccagac tttacagcag atgcgtgata
    35941 aaacgccgct gtcattaaat acgccgcccg gagaggtgaa actgaccctg gcaggatgtg
    36001 aagagcgaaa tgcgcagggc atgtgttcgt tggcaggttt tacgcaaatc gtgaatgaag
    36061 cacgcatacc ggcgtgcagt ttgtaatgca taaaaaagag cattcagtta cctgaatgct
    36121 ctgaggctga tgacaaacga agaactgtct aatgcgtaga ccggaaaggc gttcacgccg
    36181 catccggcca ctttcagttt tactctttct cggagtaact ataaccgtaa tagttatagc
    36241 cgtaactgta agcggtgctg gcgcgtttaa tcacaccatt gaggatagcg cctttaatat
    36301 tgacgcctgc ctgttccaga cgctgcattg acaaactcac ctctttggcg gtgttcaagc
    36361 caaaacgcgc aaccagcagg ctggtgccaa cagaacgccc cacgaccgcg gcatcactca
    36421 ccgccagcat cggcggcgta tcgacaatca ccagatcgta atggtcgttc gcccattcca
    36481 gtaattgacg catccgatcg cgcatcagca gttcagacgg gttaggtggc acctgaccgc
    36541 gagtaatcac atcaaagcct cctttgccaa aatgctggat cactttgttg agctcatctt
    36601 tacctgccag atattccgac aagccatgtt cattactcac ggtaaacagg ttatgcgaat
    36661 aaccacggcg taagtcggca tcaataaata acactttttg atcggactgg gcgatcaccg
    36721 ctgccagagt tgaactgaca aacgttttac cactgtctgg cgtcgcaccg gtgatcatca
    36781 gaatgttatt ctccgtctcc atcatagcga aatgcagact ggttcgtagc gcacgtacgg
    36841 cttccacagc agaatccgcc gggttatcca ccgccaggaa ggggatattt ttagtacgat
    36901 ggcgctgctg attagaaaat aaatttttct tacgcagacg ggtgcgttta tccagccact
    36961 cggacattgg gatagtggca taaacgctga tgccgtgctc ttccagttgt tccggggctt
    37021 ctacaccacg acgcaacatc gcacgcgcca gcacggcacc cacagaaata aacaggccaa
    37081 gaataaaacc aagcaccacg ttcaacgctt ttttcggttt cactggctgc ggctgagtga
    37141 ctgccgggtc gataatccgc acgttaccaa tggcactgga tttcgaaata ctcaactcct
    37201 gctggcggtt aagtaattgc agatataccg cacggcccgc ttctacgtca cggcttaaac
    37261 gtaacacttc ctgttgagtg gaaggcattg ccgatacccg cttattcagg cgtttgcgtt
    37321 cttgctccag cgtctggcgt ttttccagca gcgcacgata agttgggtga tctttcttat
    37381 acagctggga gatctctgcc tcgcggaagg tcagctcatt gagttgatta tcaacgttca
    37441 caatctgctc aagaacggct ttggcttcca ggttaaggtc aaccgaatcg cgctgctggc
    37501 gataaacgtt gagtttttct tccgcctggt ccagctcgct gcgcacttca ggtaactggc
    37561 gctgtaagaa ttcaaggctt tgtgaatcct gcgccgcctg gcgagcgata ttctgttgca
    37621 aatagttgtt agcgatgctg ttcagaatac gagtaatcaa ctggggatca tcaccagtca
    37681 tggtaagttc cagcatcccg ctttctttac tgcgttcgct aacggtaaag gtttcctgca
    37741 atgcgttaat cgcttccagt tcggtacgct ggctcaggac aaactgtgtt cctggtttgg
    37801 ccttaatgtc cgcgatagtc agcgcaacgc catctttttc cagacgctgg ccgaccatac
    37861 cattgacggt gaactcttca ccttccagcg tatagtggcc gttttcccca accgtgagtg
    37921 tcagttgctg atcctgacca ttcagttgtg gaatatgcat ccagctgatc gccagctcac
    37981 ctggtttctc tttggttaat ctcgcccagc cgcgacccac aatcggaaaa tacttctgct
    38041 caaccatgtc gcgcagattc agttcagcaa tggttttacc gagaatcatg cgcgattgca
    38101 gcagttggat ctccggtgca gactcgggcg atgagttagg gatcatatcg ctcaggtcac
    38161 tgagaatggc gttgccctgt ttctgctcaa cctggaccag agtatctgcc tgataaattg
    38221 gcgtgcttaa cagcgagtaa gcgacagcga tcagcgtgaa taacgcggtc acgctgataa
    38281 taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc tcattttcct
    38341 gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtatcttat aaactcaaat
    38401 taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatacc gtagacatgt
    38461 tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc cagccattgc
    38521 ccaaacagca ttgtcttccc gcggacctcg ggagcgattg cggtaacctg agcaatatgt
    38581 tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt gagcttacgt
    38641 ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc tgtcgcatct
    38701 gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc cggcagacgt
    38761 ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca aaccaccagg
    38821 attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct gtcaggtcat
    38881 gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga gccagcggcg
    38941 cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg agtatcattg
    39001 ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc tgcttgtttt
    39061 gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc ataccctcag
    39121 cgttacccag cgcttccgcc agggtcatac cgctgcggtc cattttcagc gtgctctgtt
    39181 tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata aataaaatat
    39241 cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc gcatagaggt
    39301 tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct gcatctgcgg
    39361 tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg gtaatcggct
    39421 gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg aacgccgcga
    39481 cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta atctcgttgc
    39541 gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg tagaaaatcg
    39601 caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc cctgcggggg
    39661 tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc ccgatacgat
    39721 attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata atcggtttgg
    39781 cgcgcaactg atcaatcaat cctggcgtga cgggatagat attgaccatt ttgtccaggt
    39841 catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa ttctgaccag
    39901 gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa aacttaaaaa
    39961 tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt acgtcaggtt
    40021 attgtccaac cggtacttct cgctctgccg tcattggata gagctggtat ttgcgtgaca
    40081 gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg ttgcggttag
    40141 gtccaatggt catcaagtcg aatggaatcg agatataaaa acctttacta aagccgcctt
    40201 cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc gccacgccgc
    40261 tgtcaaagcg tttggcgacg tcgatcgttg cgcctttatc ttttgccaga tattgcccaa
    40321 cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc gtcacgaaac
    40381 ctgttggcgt ggaataatcg gtgaaacgca tcatgttgtc ccagtcacgc tgcttcacgt
    40441 agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaagc agctcggaac
    40501 cgacaccggc atacatcgtt tccagataac cgccatacat ctggccatag aaaccattgc
    40561 ctaagtcagc aaaatagttc gcctgcaagt tgttgagata aacgtcattg cgaacgtagt
    40621 cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggcaacagc gaagacttaa
    40681 acttgtcgta gttgttgtaa atattggtga aaataccgcc atcaagcagc aggtggtcgg
    40741 taaaccagta gcgggcactg gacatcagcc ccagctggaa catatagaaa tcttccgggc
    40801 cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct tcacgaatac
    40861 ggtagccccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt tgaagttgct
    40921 ctgattgacc aggcgctgta ccagccagct gtttgcgcag gctggctacg tcggtttcgg
    40981 tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca acgccttgcg
    41041 gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagaa tcacggtatt
    41101 tgtattgttg accagacata tacagcgtct tatcgcgcag ctgaatttcc ggtgcttcaa
    41161 aacctgcgtt gtacttcagc gcggtaagtt ggttcgctac tgtggtgtac tgcaatcctt
    41221 cagattcagg cgcaggttga tatgccggtt ttggcgtatc gcgcagggca gggcgcagat
    41281 cgttgaaatt ggtccgtaac gtgaagccaa acatcaacgt gttaccgcgt tcataactca
    41341 ggttgagatc tgcccagctg gcagcgcgat aaactgcgcc gacgttgaaa tggcttgcct
    41401 gaggcagttt gccagcgaaa tcattctggt aattgttgcc atcgtattcg agtttcagac
    41461 gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc gggccacgaa
    41521 agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga caatatttat
    41581 cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag ccccatgcca
    41641 tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatac tcaccgtcaa
    41701 acagacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc cagtaacctt
    41761 cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga tcgccgctga
    41821 aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg gtgccttcca
    41881 gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta tcccggtagt
    41941 tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac aatcctgtgc
    42001 cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact tcagcatgac
    42061 aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc ttcatcagtc
    42121 aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat atttttcggg
    42181 cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt ccacatgtct
    42241 tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa tcaccataac
    42301 gttattcttg tccgcgcctg caaggcgggg gtgatcttgc agataatcgg taacgctacg
    42361 gcctgcctgc cagggcaatt gcccggcccc agaaaccgca cccagcagag taatagtcac
    42421 cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc tgttctcatc
    42481 cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat tgagattgag
    42541 taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt ctgcttccca
    42601 ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt tcgccgcaga
    42661 gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt gtgtcaccaa
    42721 ctggacaaca ttttccatgg ggccaacgga aagtgtctgt tgttcgccag gcagataaat
    42781 agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc tggcaatgaa
    42841 atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac gggaataacc
    42901 cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc gacccagaat
    42961 gtgttttgcc actgtttttc tggccgcgtg gaagtcacct cttcctgcca gacattacac
    43021 agcacattgc gacccgcaat attcagcgtc tctaatccag caaaggaaaa agtagaactc
    43081 agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt ccagcggctg
    43141 ccatctttta ttgccagcgc gttacgcaga gggtcctgcc cggaatttgt cacttccagc
    43201 agattattat taagtttgac ggtttttaaa agacgtccat tgtgggtaac cagcatggcg
    43261 ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac cacaaagatt
    43321 cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg gatctgctga
    43381 tccgctacgg tgatatcctg attatcgaaa aggctggcgc gaaatgtatc gaccatactt
    43441 tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat aagagggcgc
    43501 acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc ggtacgaata
    43561 tcgcaaccgg gtatcattta actttaattt cgacg
//
LOCUS       DAJOSY010000024        42934 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJOSY010000024 DAJOSY010000000
VERSION     DAJOSY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.024"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            916..1482
                     /locus_tag="OHN27_001464"
     CDS             916..1482
                     /locus_tag="OHN27_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCQ2285939.1"
                     /translation="MPKSYTPNWFFTALLDNHINQLVARYSRLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWLDRQRVKKIY
                     PFAERITECWQSITHNSGSAHRCTYQPHYTYNINIPVRHNDPGSIDNIRGVLHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRKPHF"
     gene            1740..2300
                     /locus_tag="OHN27_001465"
     CDS             1740..2300
                     /locus_tag="OHN27_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285940.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAKPYQKQITRDVVLDRRSPDCEALFKDLIWAF
                     SDFARDRRGVRDPEARCLAGNPGWQC"
     gene            2369..2599
                     /locus_tag="OHN27_001466"
     CDS             2369..2599
                     /locus_tag="OHN27_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2285941.1"
                     /translation="MLTTISHDSVLLRADDPLIDMNYITSFTGMTDKWFYRLISEGHF
                     PKPIKLGRSSRWYKSEVEQWMQQRIEESRGAA"
     gene            2867..3367
                     /locus_tag="OHN27_001467"
     CDS             2867..3367
                     /locus_tag="OHN27_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285942.1"
                     /translation="MQITEALLSAPGEIRRFVQQGVDHWPRLLAFHFTLHSAEGNING
                     QQIHAFCTSFYERITERNHTTSPSSPVVLRWLREQHGGATMRCLLLLSQTSICHPRAS
                     VTVDEQCSQVVDLLQQTWRGMDTGGQCRVERCFRVTRPDTSEQYVALKTAVQSLMPLV
                     IATIIR"
     gene            3456..3641
                     /locus_tag="OHN27_001468"
     CDS             3456..3641
                     /locus_tag="OHN27_001468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285943.1"
                     /translation="MRTRIDYLADKYCFTELNESPRLRRQWQDVLDECRQTEAGPEER
                     LRIALLNVDYVSNAESG"
     gene            3619..5298
                     /gene="tssF"
                     /locus_tag="OHN27_001469"
                     /pseudo
     CDS             3619..5298
                     /gene="tssF"
                     /locus_tag="OHN27_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
     gene            5262..6107
                     /gene="tssG"
                     /locus_tag="OHN27_001470"
     CDS             5262..6107
                     /gene="tssG"
                     /locus_tag="OHN27_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCQ2285944.1"
                     /translation="MERESQPARTGLTQALALNNDIWRASFYRFCQLLELENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDIAQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIDNRSGLGAGNRIRLSQRATLGKTAGEACSRLLMTLETADPDEAEGWLPTDNRSR
                     IYRLS"
     gene            complement(6067..6941)
                     /locus_tag="OHN27_001471"
                     /pseudo
     CDS             complement(6067..6941)
                     /locus_tag="OHN27_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6948..7340)
                     /gene="tnpB"
                     /locus_tag="OHN27_001472"
     CDS             complement(6948..7340)
                     /gene="tnpB"
                     /locus_tag="OHN27_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCQ2285945.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDTLTQYIQDELRSTWHEGAAFVFV
                     NKVRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWGWQNEPELRPENTQNTLLTQ"
     gene            complement(7327..7656)
                     /locus_tag="OHN27_001473"
     CDS             complement(7327..7656)
                     /locus_tag="OHN27_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCQ2285946.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            7786..8458
                     /locus_tag="OHN27_001474"
                     /pseudo
     CDS             7786..8458
                     /locus_tag="OHN27_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(8495..8650)
                     /locus_tag="OHN27_001475"
                     /pseudo
     CDS             complement(8495..8650)
                     /locus_tag="OHN27_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            8858..13816
                     /locus_tag="OHN27_001476"
     CDS             8858..13816
                     /locus_tag="OHN27_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCQ2285947.1"
                     /translation="MKYQIVGGAGLHRSETKTVDMMVKQLPDSWFGYAGLVVTDSQGS
                     MEIDMLIITADRLLLVELKEWNGNITFEGGKWLQNGKSRGKSPYQIKREHALRLKDLL
                     QEELSRKLGYFLHVEAHVVLCGTAGPENLPLSERRYVHTRDEFLTIGNPKNYEKLVQH
                     TNFFHLFEGGKPRPNSDEALPIIKSFFEGPKVRPLPLKESGYLANDKPFFSHPHMVYN
                     EFRATHKDNSQHRGLLRQWNFDALGVANAMQTLWAEIALRETRVGRLVRHGSATMQDY
                     MLRAVRELSEEDITDDARELYELRRSFSRLDEILDSEADGWSKSERIDRVRALLAPFS
                     ELHSLGISHCDIDPHNLWYAGDQKSIVVTGFGAASLEGHNSLESLRPTLQSAPYILPE
                     DAFEEAVEPYRLDVFMLAVIAYRICFAGESLLTPGQMPEWRAPLTDPFSGILNSWFEQ
                     ALNLEPSKRFPRADIMLNEFNAATKEHSQEFDEANQIYQELKQNKFFREGMNSVGVLI
                     EFPPLPEQLSMVYSALAAIATTGSISYHCEQGGKALQVKLWDGVILTPQQPGVNRRIH
                     AFKQRIDKLTHINLPTPKVQSYGLLGQGGLYVVSEYVDGLPWSQFIAENVLVQSQRFT
                     IAEKLINTIHAFHEKQLPHGDLCPEKLLVQVGEQTVITLIGLLEFSDELTADNRYQPE
                     NPESTDAFGRDCFAVYRMVEELFSEDMPVLVQAELERAKQTVDGIPIALDPLLQSIRA
                     PEQAEINQVVASESQDKVIPVCWGTDDWPQEVKLLEQNDGIYYFQCNWSSNPRFAHEL
                     RCYITGLGERLLIDLDPDNRTINRIVYEKGLSIEESIKAGKYSQAKINTQLSLQRGSL
                     NQRNTFIELLFNLEPVIDAIIERANPNQEMDEDDFDSSESSPIELWQALSDTEVDLRD
                     IVNIDSTDFQESPSGCLLYPYTTESGADLSFELDDKIIVYIKDKRESVQLGELQLSET
                     TPSLLAIRFDFDAARKRISSGSQLQLESIRDKSSRELRQRALQRVIENKAEIQHLPQY
                     FDYHQKPCMQQMQPRPSAETLRALYDQPGQRFNEQQLMAFQQLVEFGPVGVLQGPPGT
                     GKTTFISKFIHYLYQHCGVNNILLVGQSHASVDNVAIKARELCHTKGMELDTVRIGNE
                     LMIDEGMLSVATKALQRQIQHKFHREYDLRVSSLGKRLGMAPLLVQQLCQLHRTLNPL
                     MVTYGQYSRELDKVEQIKSSSISHQERLAELLEQSNQLKLRTQEIINSIFDDSLLKTL
                     VYDETLIRQLAEQVAIQYNYNNPENLERFMQLLEMSQEWMDVLRGGEAGFDRFMFKSK
                     RLVCGTLVGVGNRRLELAESSFDWVIVDEAGRAQAAELMVALQSGKRVLLVGDHKQLP
                     PFYHQQHLKLASKKLELGKGIFYESDFERAFKATGGVTLDTQYRMVEPIGELVSECFY
                     AQDIGKLHSSRKVSPDWYSKLPIPWNKTVTWIDSSSPNEAGAEEHKGNGRYYNQREVR
                     LLLEALQSLSSDGCIAQLEQTITTEQPYPIGIITMYRQQKEEIDNAISRAEWAASLRG
                     LIKIDTVDSYQGQENKIIILSLVRDNPNKLQGFLRDAPRINVAISRAQERLLILGARR
                     MWSKTNNDSALGNVHEFISKQVAVDEPNYQILCGQSLLGDNN"
     gene            13816..15363
                     /locus_tag="OHN27_001477"
     CDS             13816..15363
                     /locus_tag="OHN27_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285948.1"
                     /translation="MSEPRLGNLITVLLPARSYKINCALTTEKLMPGIEQFACRLLLI
                     FDQLYPSELQNYFGLTDREREVLLDGLLANRLININPDGHIEASSFLRKHAANNGGKP
                     SLVKYQECTEEVAFDLLTLSICKPQPNRRFTSGLPELLPRHQIGGDAAAVTEAFSSQF
                     RHHLLLSRNSEYERQRTKLYKIMGCSSHEMVQLPIEIEVSYGVSAGSIEPQKFTRSYE
                     YLGNTRLPLSNELEAHIADFLGEHKLDEFGIDCEDFCKLANDKVLLQFANGYKFNYSG
                     WIEAREQRKTGYGTSLTTGMLGAVYLPHNSKLFISMLHNALRDYIGKTAPKALWYSSK
                     VPLWGANGSQLSRFTRALGDILGNYADDKIARISLLHSSADEGEKRQERKRHLGRFPT
                     GIGLTSEAKFDRLEILLIPDVIALVQYHGQPNSDSALTLPIGYITVEPERLELLKKLM
                     IKRTEGAVATITWSESKFENLASLLPVEFLIKLNKKSGEDVDAAIKKMQIYNRAETAR
                     AILSLRK"
     gene            15578..16768
                     /locus_tag="OHN27_001478"
                     /pseudo
     CDS             15578..16768
                     /locus_tag="OHN27_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    15859..15975
                     /locus_tag="OHN27_001478"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(16915..17211)
                     /locus_tag="OHN27_001479"
                     /pseudo
     CDS             complement(16915..17211)
                     /locus_tag="OHN27_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(17191..17289)
                     /locus_tag="OHN27_001480"
     CDS             complement(17191..17289)
                     /locus_tag="OHN27_001480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2285949.1"
                     /translation="MKKRFSDEQIISILLEAKAGVSARELCRALDQ"
     gene            17372..17474
                     /locus_tag="OHN27_001481"
                     /pseudo
     CDS             17372..17474
                     /locus_tag="OHN27_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705337.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            17729..18658
                     /gene="dcm"
                     /locus_tag="OHN27_001482"
     CDS             17729..18658
                     /gene="dcm"
                     /locus_tag="OHN27_001482"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HCQ2285950.1"
                     /translation="MKKKPLKQYKVSSFFAGIGGFDLGLEKAGMEVVFQCEINKFCQK
                     VLRKNWSKVPLHTDITRLNADEIPESNVWCGGFPCQDVSSANQGKRKGLEGARSGLFY
                     TYAKLIEERKPEWLIIENVPGLLNSHNGQDFKVVIDTLVEFGYGVSWRVLDAKYFGTP
                     QRRRRVYIVASLGDMRSARVLFEPGATRIVDKQGQGERANASGASATGLSKADIYSIQ
                     HASIGRKATAGPQAKGYRNDGETYTLDSRGSSDAVCAPDAAFRVRASTGISRELDSNR
                     FRAVGNAVAVPIVEWLGKRIVLVDQQVLNEALS"
     gene            complement(18639..19595)
                     /locus_tag="OHN27_001483"
     CDS             complement(18639..19595)
                     /locus_tag="OHN27_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285951.1"
                     /translation="MAVSIAEYLGQRTDVDQQIVPVAKGTQIQCPFMDRTCDKASKVK
                     NPTPPVCSVRKPDGTVWIVCEHRLCSTRQKKTVIVNGRKKQIENILVEHQRDILRKVA
                     KLIYQDPELQDSEIGVRREVNIPLPDSDNSYHADYVMRNFSGRGRVDEVLLEMQGGGE
                     TSSTGEITRHIAAWADLEFPTNEILRQPVAANTIETNAWRRQQEQFLVKGNVVDQTGG
                     KIVFAVGSLLYDYLHRRFRNANLRDLKAHNWTLCILAFKEDTSDEPRPGPIPLIIDDS
                     KTLFTNYSTFVRFLTDQGAPRPELFEGSFLRLDNSSVTIMPR"
     gene            19854..21082
                     /locus_tag="OHN27_001484"
     CDS             join(19854..20169,20169..21082)
                     /locus_tag="OHN27_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCQ2285952.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDV
                     LLRIHHVIGELPTYGYRRIWALLRRQAELDGMPAINAKRIYRIMRQNALLLERKTAVP
                     PSKRAHTGKVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFD
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRASNGLSDDRCLEI"
     gene            21339..21758
                     /locus_tag="OHN27_001485"
     CDS             21339..21758
                     /locus_tag="OHN27_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCQ2285953.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACVVQIARENGVND
                     NVIFKWLRLWQNEGCISQRLPVTTTSDAGVELLPVEITPDEPKEPVAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            21755..22105
                     /gene="tnpB"
                     /locus_tag="OHN27_001486"
     CDS             21755..22105
                     /gene="tnpB"
                     /locus_tag="OHN27_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCQ2285954.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            22136..22897
                     /locus_tag="OHN27_001487"
                     /pseudo
     CDS             22136..22897
                     /locus_tag="OHN27_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23126..24358)
                     /locus_tag="OHN27_001488"
     CDS             complement(23126..24358)
                     /locus_tag="OHN27_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2285955.1"
                     /translation="MNKWMKLCFLILGGGTIFKLSSMKDVFYVPMQADWGLTNTQIGF
                     GFTIYAAVQTIGLFSSLYIADRFSKKILLPAGLIGVGLCGAYLTTLPPFSGYLIAFGA
                     MAFFGEVVYWPVLLKAVRLLGNRDEQGRMFGFLEAGRGVVDVLIASGALFVFVQFGEG
                     KAGMQAGLVYYTLITIIVGIITYFIVDADDRTIPQNDEDVNKQIFKGIKNVVKSPNLW
                     LASFCIFFVYSAYCGLTYFIPFLKDIYALPVALVGAYGIINQYGLKMVGGPVGGYLAD
                     KISHSPVIYLKWTFLISAVAMLLFIQLPHDSMNVYLGMTATLGFGAIIFSQRAIFFAP
                     MDEIGTPREFAGSAMAFGCIIGYMPSMFAYTLYGSLLDNFSGIQGYNYVFSVMVAFSL
                     LGFICATLLTRRMKMRNE"
     gene            complement(24373..24750)
                     /locus_tag="OHN27_001489"
     CDS             complement(24373..24750)
                     /locus_tag="OHN27_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCQ2285956.1"
                     /translation="MIIDTKHAPKAIGPYVQAVSTGNLLFISGCCPFKPEDGTVNGET
                     IERQTRQAMDNLKAIIESAGIGMDNIVKTTCFISNMDNFSQFNLLYSEYFSSDKYPAR
                     SCVEVARLPKDVLIEIEAIAVIK"
     gene            complement(24772..25731)
                     /locus_tag="OHN27_001490"
     CDS             complement(24772..25731)
                     /locus_tag="OHN27_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCQ2285957.1"
                     /translation="MKLGLETESYHLFFQQGMMDIFDFIDKTSELGLDGVEINIIPDV
                     GLHPEFGVLNSISPEYLSKVRKSIDSHGLYCEIDTRFTSCEAITRAVEIASALGAEVI
                     RTYMFRRGEYNPLKYPEIVEQLKSLTSLLSRKKIKLAIENHEDETADEIIEVIKSVNS
                     EWVGAHCDIGNGMMAWEEPVETVRKLAPYAYSTHFKDHIVTRNGEDLVVCGVPVGEGS
                     IDIDTCFKTLVKESSLTRINIETCFPYASPFARPKGTGGVYSLEGAFSVKEAPFEHLK
                     IKPLDYYYPAKISDSVLQELMAAQNRCVEVSVQTLKNLRKKYC"
     gene            25917..26579
                     /locus_tag="OHN27_001491"
     CDS             25917..26579
                     /locus_tag="OHN27_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCQ2285958.1"
                     /translation="MKSIVNHEFTEDDRQLLLSYIPFIDSLGQFLGEYCEIVLHSFED
                     LHHSVIHIVNGHVTGREKGAPVTNIALEKLAEFNSTNDMWDVYFNHKGVRPFKSSSSL
                     ILNKNKEPVGMICMNFALDMPLNAFIENFAKSSNCKNENFTQDINNLVLSHLEPVKNM
                     VYSNKNIPSKNKNFEIVKELNSIGLFKFPVTTKIVSSVLGISANTIYKHLRVLNNKGK
                     EN"
     gene            complement(26697..26843)
                     /locus_tag="OHN27_001492"
     CDS             complement(26697..26843)
                     /locus_tag="OHN27_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCQ2285959.1"
                     /translation="MFSKSATELRPKLPALLEDAAMLYLRIGTCRLNNMAPEKWLRYV
                     IEHI"
     gene            27512..28432
                     /locus_tag="OHN27_001493"
     CDS             27512..28432
                     /locus_tag="OHN27_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCQ2285960.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDGLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISGESQVQQGAYSLVQRYSLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IVQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            28600..30117
                     /locus_tag="OHN27_001494"
     CDS             28600..30117
                     /locus_tag="OHN27_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCQ2285961.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNDTFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            30178..31548
                     /locus_tag="OHN27_001495"
     CDS             30178..31548
                     /locus_tag="OHN27_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCQ2285962.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADIARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNTDNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLFK
                     YKTDEE"
     gene            31548..32957
                     /locus_tag="OHN27_001496"
     CDS             31548..32957
                     /locus_tag="OHN27_001496"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCQ2285963.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVISLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLNTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDLFALDGEYILLCCPQGMVREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            32983..33990
                     /locus_tag="OHN27_001497"
     CDS             32983..33990
                     /locus_tag="OHN27_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2285964.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSTIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(34542..35015)
                     /locus_tag="OHN27_001498"
     CDS             complement(34542..35015)
                     /locus_tag="OHN27_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HCQ2285965.1"
                     /translation="MCKLTKEEEYSIISRTMTNIRSLRTYVRELDFEQLLDMQEKLNT
                     VIEERREDAERKAAERKELETKCQQVFEYIVSVGLDPEEWQGPVSAIAGTTETKRKPK
                     GGVRKAKYIFEDENGETRTWSGNGKMPLALRKQVNGDCTLETFLIEKPNQHEQSE"
     gene            35334..36071
                     /locus_tag="OHN27_001499"
     CDS             35334..36071
                     /locus_tag="OHN27_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HCQ2285966.1"
                     /translation="MKKVIAVSALAMAGMFSAQTLADESKTGFYVTGKAGASVMSLSE
                     QRFVDGEGAWADKYKGGDKSDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGNAESKY
                     RLSYWESVGGAEFDDAQNKLSVNTLMLNAYYDFRNSSAFTPWISAGLGYARVHHKTSY
                     IYTDNSPAGSEVYSASASKYENNLAWSLGVGVKYDVTQDFSLDLSYRYLDAGDSTLTY
                     KDEDGAKYKSSVDVRSNEFMLGATYNF"
     gene            36491..37387
                     /locus_tag="OHN27_001500"
     CDS             36491..37387
                     /locus_tag="OHN27_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCQ2285967.1"
                     /translation="MADSTRNRSAERLIDILVELQNYGVVSRHNLMKKYNITERTAYR
                     DLNMLSPFIEACGDGKYRLISARAGNQSKGSLHKSLARLLDTDAIFPERDEGFWQKLE
                     NRATEKHIRVQFHNPEHTIRDDLRKYLDVLEKAIRNSNICQIAYAGKTRIVHPYKLTN
                     QRSIWYLLATEENKLKSFSLAKIKWLDIKKEKFAKSEEIQSLISESCDPWVSDKTFDV
                     VLFIHSNIAHYFLRRDLLPYQQLLHRHDNGITLSCKAAHKNQIIPLILYWLPNVEIIE
                     PVWLKEAVLTMLGKYLTAENVS"
     gene            37436..38515
                     /locus_tag="OHN27_001501"
     CDS             37436..38515
                     /locus_tag="OHN27_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCQ2285968.1"
                     /translation="MALPFIILGVAAGAAAFGGKKAYDGYQKKSEAEDVLEKAKERYE
                     EHKGKFEQAEAQTSSKLTTLGNKELEIGKDFSEFDRIAAELLARLEKEGNKDLKLSVP
                     QHNINKIHNLAISATEYLSTVVAAGVSGAAAGFAVYSGVMAFAAASTGTPIAALSGAA
                     AYNATMAAIGGGSLAAGGMGMAGGAMVLGTAVAAPLIAIAGWAYDRHATKALENAHQC
                     ACKVERAVKKMNLAREHFAKVNQYVDEILAALNRMHAVFIKHYFEPLKSMHSVITEQQ
                     DINVSDEIIMLIDHGYSLAAIMTDIITTPLFKPRKQENGEAVIKDNVIEMEKDDNGMN
                     VINKEELDDVLASSVVKFDDFSSRS"
     gene            38562..40133
                     /locus_tag="OHN27_001502"
     CDS             38562..40133
                     /locus_tag="OHN27_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HCQ2285969.1"
                     /translation="MNVEKFDWAEELHQTMVKSLVASFGLDFLLLNDRKGGDVDTIHN
                     VRNGIYATEAERQRYEGRGDYDSHHYHSHENYIATNRKGKRAHQSGTLTDAYTGEVFA
                     SDDKKNLDHIISAKEIHDDPGRILAERDGAELANDASNLAFTHESLNKSKKADSMDAF
                     ICTLQKNREDTLRQIAELESQATLTEKEKKRLISLRKKADADTERMERADKYAREKYE
                     TTINQSYYLSSKFATAVASASLNSGFRMGTRQMLGLILAEIWFELRERIPHIIRQQRN
                     SFCAGKCLSEITSALRACQERVQEKFSSFLIAFKDGVIGGILSGITTTLLNIFLTTER
                     MIVRLIREMWNNLVQAFKVLVFNPEGLSPGQLAKAVTKLIAAGVAVAGGVMINEAMAQ
                     ILIFPFGSELAAFCGALTTGILGLVMNYYLEYSEVMQRVWAFLDKFKDKFQRSLEYFQ
                     AVNAELDRYLVELTALEFSVDAAALNHFSLHLDSVNSEIERGLLLREEVSRRGIALPF
                     ESGNVASVRDWLNKR"
     gene            40536..40733
                     /locus_tag="OHN27_001503"
     CDS             40536..40733
                     /locus_tag="OHN27_001503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2285970.1"
                     /translation="MRLLLKGLVGMALVWLVIGFGMELFFLADRVHPALRVIVGLGML
                     FLMIMIAGGMCRSLLSRKKTS"
     gene            40786..42459
                     /locus_tag="OHN27_001504"
     CDS             40786..42459
                     /locus_tag="OHN27_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCQ2285971.1"
                     /translation="MNSPVTACSATSAGQKYRFYTIIMKINLPFIHWPRICNDKCLVL
                     SVGVFTLSNLLVSSGAHLIYRVLFFILAILIIKAMKSLTLRFILAFPFTLLTAADISI
                     SLYSWCTFDTTFNDGFAISILQTDPDEAIRMFRMYVVYVIAFIILFLLFFCSAINKTS
                     SLPSEKVTVITFLLLITVTLYSSFQFALKKQYQINEVDPYIVASRFATYTPFFNLNYF
                     ALAAKEHQRLMTIADTIPHYDLVITDNNTDVFVLVIGESARTDNMSIYGYSRPTTPEL
                     QKQKSRLKLFTQAISGAPYTALAVPLALSADSVLHHDIRNYPDNIINMANQAGFDTWW
                     LSAQSAFRQNGTAVASIAMRARNRIYVRGYDELLLPHLAEALNSNPGSRKLIVLHLTG
                     SHEPVCSNWPRDKAVFKPLDTEEVCYDNSIHYTDSLLGQVFTMLETRQASVMYFSDHG
                     LEYDPTKEHAYFHGGIKPSQQAYHVPMFIWYSPTLGEHVDRQTVNSVFSTAYNDYLIN
                     AWMGVTRPAQPKTPEEVITRWQGKSVVFDASHNVFDYKVLRKNFNELQE"
     gene            complement(42603..>42934)
                     /locus_tag="OHN27_001505"
     CDS             complement(42603..>42934)
                     /locus_tag="OHN27_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCQ2285972.1"
                     /translation="TAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAEIIKCAE
                     MLMQEDDFEICESILRQQKLLRERIDETLSEEIVQKCRNINGEYVWPWILPFSAAGMK
                     HSSIQHQ"
BASE COUNT        11458 a         9395 c        10374 g        11707 t
ORIGIN      
        1 tacttcctgt cttcactcac tctcaatctt tacacattcc attagtatta ctcttgttat
       61 cgtattcact ccatccctca atcataacta ctgtaactca tatgatgttc aggtaagtta
      121 ttctctacta cgctattgtt gatattcatc tattctcatc ttcagtaaaa cagtaattga
      181 ttctaattta tccattatga accgtatttg cttaacaatg attgtttatc tgaagtgttt
      241 taactattct ggtgaacatc aatttctctg tcattactga ttaactgaat gcttactctt
      301 taataaggta tccattattc tgtaacgatt agccgagcag ggtaaataac agaattaata
      361 gagtgaattt ctgattgtgt ttgttcgttc ctgattatct tgtattttaa acaaaatgaa
      421 aattatccat ccatggtatg tttcattctg gtaccgtgta cccgttaacg ttcatatctt
      481 gaacatgagt tgtttttaac agataaaatg agtgcgtgta agtttattgg gttacaggta
      541 tcgtgttggc cttgtacacg gttttttaga accattgaga gcaagtttgt aatttatgat
      601 ggtgattagt cgtaatatgt ttcttgaact tctgatcgtg ggtttttatc gcatattttg
      661 cagtatttcg tgatgtttat tgattctgta ttttattcac tcctcgttta tctcatctct
      721 ttagctaata ccatcagata atccatttat ttctgcacaa cgctacgtgt cgttaataac
      781 cgacgtatcc attctgttac agcatgcctg ataaataccg tctgtaagtt attaccgttt
      841 tagatctgat tatgagcgaa agcattaatt cgtttacagc acttaaaacg tcattaactt
      901 tcaggagtca tcagcatgcc taaatcttac acaccaaact ggttttttac cgctttactt
      961 gacaatcaca tcaatcagtt ggtcgcacgc tattcccgcc tgcgggcctt acgcatggat
     1021 ttcttctaca ggaaagatac gcctgatttc ttacaacctg atcatcgctg gcttgaattg
     1081 cagttgcgta tgctgctgga gcaggtggaa caatttgaaa atatcgttgg tttcttctgg
     1141 gtgattgaat ggacggctga tcatggtttt catgcgcatg cggttttctg gctcgatcgt
     1201 cagagggtta aaaaaatata tccctttgcg gagcggatta cggaatgctg gcagtctatt
     1261 acgcataaca gcggttcggc acaccgctgt acatatcagc cgcattatac atacaacatc
     1321 aacattcccg tgcgtcataa cgatcccggg agcatcgata atattcgcgg tgtcctgcat
     1381 tatctggcga aagaagagca aaaagacgga ctgtgcgcat acggttgcag tgaagttcct
     1441 gaacgacctg ctgcagggcg tcctcgtaag cctcacttct gaagcttaaa tcctgagctt
     1501 cctgtcctgg aaacattccg tcggtaaaac cttaccgcct tgcttaatga tgtgaactga
     1561 agtcaacgga gatcattcac tctgaaccac atccggtgtt ctgtcccttg tcggtgacct
     1621 gatcttcccg ttctgccccg gttcttcact tattccatca accccatccg caaaccataa
     1681 cacgtcagct tattcacggg caggacgcat tgtgggctgc gcataacgga gcatatctta
     1741 tgaatgctat cccttatttc gactatagcc tggccccctt ctggccatct tatcagaaca
     1801 aagtcatcgg cgtccttgag cgtgcgctgc gtgagcagtc cggctcacgg atacggcgga
     1861 tcctgcttcg tctgccgtgt gaatatgaca ataccttcaa cagcagaacg acctggtttg
     1921 gtatggattt tatcgaaacc gtcagtgcgc tgatgaatgc aacacccgga cgtgaccttt
     1981 gctggctcct gacccgtcat cctgaaaaac cggaatacca tgtggtgctg tgcgtcagac
     2041 aggagtattt cgacggcccc gaactggatc ggttgattct ggatgcctgg agtaacgtgc
     2101 tgggtttcgc gtcaccaggt gaagcaaagc cgtaccagaa gcagatcacc cgggatgtgg
     2161 tactggatcg ccggtcaccg gactgcgaag ccctgtttaa ggaccttatc tgggcgttca
     2221 gtgatttcgc ccgcgatcgc cgtggagtgc gcgatccgga agcccgttgc ctcgccggta
     2281 atcccggttg gcagtgctga aagcagcatt ccatcccata cctcgtatta ccccattctt
     2341 catcaatctc acagaggata ttctgaccat gttgaccaca ataagccacg acagcgtgtt
     2401 gctgcgtgcc gacgatcccc tgatcgacat gaactacatc accagtttca ccggcatgac
     2461 cgataaatgg ttttacaggc tgatcagtga aggtcatttc cctaaaccca tcaaactggg
     2521 gcgcagcagc cgctggtaca aaagtgaagt agagcagtgg atgcagcagc gaattgagga
     2581 atcacgagga gcagcatgaa acgagttgtg atgccggtac gctggcaatg tgcaaaatgc
     2641 cggcgctggt attgcggaaa tcagctctgt ccctggtgct ggcgacatta ccgttcattt
     2701 tcctgctgac cctctccggt cagccagcag gttgtagctc tttcctgact gattcgttat
     2761 tcctttcgtc ccgtcagtca ctggtgtttg tatcgtactg tgctggtgtt gatgtgcgtt
     2821 ttccgtgtgt ctgcaccggc ttaacaaaat tcacaggggt aaaatagtgc aaataaccga
     2881 agccttactt tcagcgccgg gggaaatccg gcgttttgtt cagcaggggg tggaccactg
     2941 gccgcgtctg ctggcatttc acttcacgct ccattcggca gaaggaaaca tcaacgggca
     3001 acagattcat gcattctgta cttcctttta tgaacgtatt actgagcgta atcacacgac
     3061 cagtccatca tcgccggtgg tattacgctg gttgcgggaa caacatggag gagcaacaat
     3121 gcgatgcctg ttgctgctca gccagacgag tatttgtcac ccgcgagcca gtgtcacagt
     3181 tgatgaacaa tgttcgcaag tggtggattt actgcagcag acctggcggg ggatggatac
     3241 gggcggacaa tgtcgggtgg aaaggtgttt tcgggttacc cggcctgata catcagagca
     3301 atacgttgcg ttaaaaacgg ctgttcagtc tctgatgcca ctggttattg caacaatcat
     3361 ccggtgatga ccgggagaaa tatcgactct tccgttaatt tttacagact gataaagttt
     3421 tgtctgctga atctttatac ggaattcttt tcagtatgcg tacacgaatt gattatctgg
     3481 cggataaata ctgttttaca gaactgaatg aatcgccccg tcttcgccgg caatggcagg
     3541 atgtcctgga tgagtgccgg cagacagagg cagggccgga agaacggttg cgtattgcac
     3601 tgttgaatgt ggattacgtc agcaatgctg aatctggata agcctggtgc ctgcgatccc
     3661 agtgtggaac gtctgtttga aggctttgcc ttcctgatgg gacgtttgcg tgaaaagctg
     3721 gatgatgacc tgccggagct caccgaaggt ctggtgagtc tgctgtggcc gcactatatg
     3781 cggactatcc cgtcgctggc catcgttgag ttctcgcctg actggcgtag tctgcgtcag
     3841 gccgaaatac tggctgaagg cttttccgtt ctgtcccgtc ctgtcgggcc gcataaaacc
     3901 gcctgccagt accgggcgac ccgcgatgtg ttgctacaac cgctgcacct tgcagatgcc
     3961 cgtctccata ctgaatccga tggccgctct gctatccgtc tgcgcttcga atgccctgaa
     4021 aaagtggact ggagtaaagc cgggatcgac aaagtcgcca tttttcttaa cgcagaggcg
     4081 ccggtaagtg ctgcgctaca tctggcaatg acccggcgtg tacatgccat gtatgcgcgt
     4141 catgccggga catataccgg acgtcgtcag tttgacggct ggtgcagacc catgggcttt
     4201 gacgacaatg actgcctgtg gaagaaagct gatacagcct tcagtggtta ccagttgctg
     4261 ctggagtatt tcagcttccg tctgaagttt atgtttgttg aactgcgcgg cctcgacacc
     4321 attgggctca ctgcggccag tacctggttt gaaatcgaca ttgtgctcag tgaagcctgg
     4381 tcatccggtt tgccttttga gacagaaaac ttccgcctgc actgtgcgcc ggttatcaat
     4441 ctctttaccc tggaagccga cccgctgatc cttaacccgc tggataacga gtacctgctc
     4501 aggccgctgc gtcttcagga tggtcacacc gaaatttaca gcgtcgataa cattcacggc
     4561 tcggtgaaga acggcagaca cccttatgtc ccgttcacca gcttccggca ccggggcggt
     4621 atgatgcgcc acgatgctcc tgaacgttat taccacaccc gtgtgaaacg gggaccctcc
     4681 ggcctgtatg acacatggtt agttctcggg gggcgttcgt tcgagctgga gcagttgtcc
     4741 gataaaccgg agtccctctc gatgcgaatc accgcgacca acgggcagct tccgcgcagg
     4801 gcgctggaaa gtacgctcct ggacagggtg gtcaaaaccg gcaaagtccc cgtcagagtc
     4861 ctgaacctga ccgcaccgac gatgccactg tatcccccgg caaatgaccg gtttcactgg
     4921 cgggtgatga gtcacctcgg atcaaacttt ctcagcatga tggacaatcc ggaggtgctg
     4981 cgggggacgc tggcgctcta tgactggacc gacgatgaaa tgaaccgccg ccggcttgaa
     5041 gccatcgttg ctgtgaagca caccctgatc cgccgttttg aaaagggctt tatgctccgt
     5101 ggcgtggaca ttgaggtcac gctgaatatg gataattttg ccggcgaggg cgatgtgaat
     5161 ctcttcgggg agatgctgca ccgtttcttt gcgctctatg ctgatgttca ccttttcaac
     5221 cagctcacac tggtactgca acccacaggg aaacgactga gatggaaaga gaatcacagc
     5281 cagcacgtac cgggctgacg caggccctgg cccttaacaa cgatatctgg cgggccagtt
     5341 tttatcgttt ctgccagctg ctggagctgg aaaatccgga cgggccaaaa ctgggcacga
     5401 ccagccatcc gggcgatgac ccggtgcgat tcaggccctg gcccgggatg ggattcccgg
     5461 tcagcaccct gaaagcggtg gagatcgacg aagaccgtcc gacattaccc ccaacgatcc
     5521 gcacgacatt tctcggaatg tatggggtgg actcacctct gccgacgtcc tggctggatg
     5581 atattgcgca gcgccgtgaa ggacatgagg cgctcacctc atttctggat attttcagcc
     5641 accgcatcac cacccagtac taccggatct ggcgaaaata tgcgtatccg gccactttcg
     5701 aggagggggg gcgcgatgcc acatcgcagt gtctgctggg actggtcgga ctcggtatac
     5761 ccggcaccgc tgaacaggtg gcaacgccgg tttcccgttt cctggcctta ctgggtgcca
     5821 tgcgattacc cacccgcaat gctgaaggca tccgggctct ggtgagtctg ctggcaccgg
     5881 atacccgcgc acttatcacc gaacccgatc cggttaaggt ccatatcgat aaccgcagtg
     5941 ggctgggtgc cggaaaccgc atccgtcttt cgcagcgggc cacgctgggt aaaacggcag
     6001 gggaagcatg cagccggctc ctgatgacac tggaaacagc ggatccggac gaggcggaag
     6061 gctggttgcc cacagataac cggagcagga tttaccgcct ttcttagtgt tcaggatctt
     6121 cagggtagtc tcatctgcat gcaccaccgg acggtttatc aactcgcgat ggagtaacgc
     6181 cgccagggga gataatgcgg cacccgccgc gccaaccatg tcagccatcg aactgacggg
     6241 cagcccgaca ctgaacgggc aaagacctgt tgctggcgat acagaggcag gtggtcaccg
     6301 tatttattga tgatcacctg tgcgatgact gatggctcaa cggcactttt cgggaggata
     6361 tgtgccggca tttcaccgct gaacaccttc tggcaacagg aacaactgta ttgcggacgg
     6421 acataacggt tcaccacgaa gtgaacggga atatactcca gcttttcact caccgcatca
     6481 cggatatagt gcagcggctc gtcacattca gggcaatggt cagtgtccgg ctggataatt
     6541 ttttccaccc gtggaagatg agccggtaag ggtttgcgta tcgggcgtga acgggacgct
     6601 ttttcgtctt cttcagggga ttgcgggagc agtttatcca gatgtgcggt aagcgcggcg
     6661 atatcgacat caacatcctc ttcgaacagg gagcgttgca taccagccag cgtttcacat
     6721 tttttaccga agcgctgttg acgaaccagt ttcagcattt cttccagaag ctggatgcgc
     6781 cggcttttct ccagtaattc ccgctctttt tcggcattct ccgacatgac tttttgtacc
     6841 atggtaagtg ccatagcacg cagtttttcg atgtcattcg tggtgttgag agcggagata
     6901 tccatacagg ggagtatacc actccagata cggatattca ttatttttca ctgagttagc
     6961 agtgtatttt gcgtgttttc agggcgcagt tcaggttcat tctgccagcc ccatttcgtc
     7021 aggtcatgtc ccttaacctg ctgccagtca acaccggcaa tcagccagtt aaactcgtcg
     7081 ggagttaggt gccaggcagc atcatttgca cgtggccagc ggaagctgcc tttgtgcaga
     7141 cggcgggtac acaaccacac cccgtgttta tcccaccgga gaactttgat acgcgaacgg
     7201 actttgttaa caaagacgaa ggcggctccc tcatgccatg tcgaccggag ttcatcctgt
     7261 atatactgcg tcagtgtgtc gatgccccgg cgcatgtcaa caggtttaat cgcgatgaag
     7321 acattatcag gactgagcat atttgagcgc cctgaagaca tcggtaagtt gtgacggctg
     7381 acagcacatc cgtactccgc cgggaaggaa gagcgtgact ggctcattcg tgacgggctg
     7441 tggtacatca gcatgcagtg gtggcgcaat atgaacggga agaacggtcg gttcatttgc
     7501 ccggcgttca gcttttgcga catcctgcgg ccattcgcga agtgatgtga agggaatatc
     7561 gttgagttca cagtattgct gacgtgtaag accactggcg cgccaggcag caacgtgctg
     7621 ccttttctgt tcaatggtcc atcggggttt tgacattgat tatctccggt aagttggtga
     7681 atgaatgtcg gtaatgctaa ctgactgaaa atctctgtga agatgacttc cccgtaacga
     7741 caccttcttt atcgcctgcc gaatgacgct gttcagcgat tcgatggcta tcgtcgtgtc
     7801 cgggctattc tcagacggca gtcttaacga aggactgcag ttcgtgaatg caaatcgcgt
     7861 ttatcgaata atgagcgaga ataacctgtt gttattacat gacaaacctt cacgaccgca
     7921 gagggaacat aaaggcccga atatcggtga aggaaagcga cccgcgctgg tgtgccgatg
     7981 gctttgagtt cggttgtgat gatgacgaaa aactgcgtgt cacgttcgca ctggactgtt
     8041 gcgatcttga atccatagac tgggccgcaa gcacgggagg ttatggcaaa gcaacggtgc
     8101 aggatgtcat gtcaggagcg atagaaaaac gctttggcga tagaccggga tggcccatcc
     8161 agtggctgac cgataacggt tcggcttata caactcatga aacacggcag ttcgccagag
     8221 agttgaatct ggagctctgc acgacggcag tcagtagccc tcagagtaat ggaatgagag
     8281 aacggttcgt gaaaacgatg aagagcgatt acatcagtat catacctaaa ccggacgtgt
     8341 taacggcagt aaaaaacctt gcggaggcgt tcgaacacta taacgaatag catcctcatg
     8401 gtgccttggt tatctctcac cgagggaata tcaacgtcac tcggtaacgt taacttaagc
     8461 tcaacggctg tctggatata gtggttcaag ggcacctaag ggccttattt acggtgtttt
     8521 cgctgattgg tttacgcggg tcatgtgctc ccgtaaagac cagaccattt tcaactgccc
     8581 atgtctgaag ttttatcagt atggctacag cctgtttgct gagtggcaca agatgtggtg
     8641 ttcgcatctt atggattcac cattatagtg acatgttcaa gatgatgata tatctttgaa
     8701 aagtgttctc tttgcgaacg gtatagaatt tctagcgtta cttttcataa ttacactttt
     8761 tagggttagg caggcacaat ctatgcgctg tcttagataa ctacatccat ttttactgga
     8821 ctaccaccaa caaaaattta gtggtgcagg agaaaacgtg aagtatcaga tagtaggtgg
     8881 tgctggcctg caccgcagcg aaaccaaaac agttgatatg atggttaagc agttaccaga
     8941 tagttggttt ggctatgctg gcttagttgt tactgatagc caagggtcga tggaaatcga
     9001 tatgctaatt attactgctg accgtctgct attagtcgag cttaaagagt ggaatggtaa
     9061 catcacattt gaagggggga agtggctgca aaatggtaag tcacgaggca aaagtcccta
     9121 tcagatcaag cgtgagcatg cactgcgact aaaagatttg ttgcaggaag agttatctcg
     9181 taagctgggt tactttttgc atgttgaggc tcatgtagtg ctgtgtggca cagctggtcc
     9241 tgaaaacttg ccattaagtg agaggcgcta tgttcatacc cgtgatgaat tcttgactat
     9301 aggtaaccca aaaaattacg aaaagctggt gcaacacact aacttttttc atctttttga
     9361 agggggaaag cctcgaccaa attctgatga ggcattacct ataattaagt ccttctttga
     9421 aggaccaaaa gtcaggcctt tgccactaaa agaaagcggt tatcttgcga acgataagcc
     9481 attctttagt caccctcaca tggtctacaa cgaattcagg gctacccaca aagacaatag
     9541 tcaacacaga ggtctgctac ggcagtggaa ctttgatgcc ttgggtgtag caaacgcaat
     9601 gcaaacattg tgggctgaga tagctctgcg tgagactcga gtcggtcgcc tagttcgtca
     9661 tggcagcgca actatgcagg attatatgtt gcgtgctgta agggaactat ccgaggagga
     9721 tataactgat gatgcccgtg agctgtatga gttacgccgt agttttagcc gattagatga
     9781 gattctagat agcgaagctg acggatggag taaatctgag cgtattgatc gcgttcgtgc
     9841 attattagct ccattctcgg aattacatag cttgggtatc agtcattgtg atattgaccc
     9901 gcacaatcta tggtacgcag gggatcagaa gagcattgtc gttactggct ttggcgcagc
     9961 ctcactggag ggacataata gcctagagtc attgcgtccg acattgcaaa gtgctccata
    10021 tattttgccc gaagatgctt ttgaagaagc agttgagccc tatcgcctag atgtattcat
    10081 gttggctgta attgcttatc gtatttgttt tgcaggtgaa tcattactga ctcctggaca
    10141 gatgcctgaa tggagagctc cattaactga tccttttagc ggtattctaa atagctggtt
    10201 tgagcaagct cttaaccttg agccaagtaa acgctttcca cgtgcggaca taatgctcaa
    10261 tgagtttaat gcagctacta aggaacatag ccaagaattt gatgaagcta accagattta
    10321 tcaagaatta aagcaaaaca aattctttcg cgaagggatg aacagcgttg gtgtgttaat
    10381 tgagtttcct ccacttcctg aacagttgtc tatggtttac tctgctcttg ctgctattgc
    10441 tacgactggc agcatcagtt atcactgtga acaaggtggg aaagctctgc aggtaaaatt
    10501 gtgggatggt gttattttga cccctcaaca acctggtgtt aaccgccgta tccacgcttt
    10561 taagcaacgg atcgataagc ttacgcatat aaatctgcca actcctaagg tgcagtccta
    10621 tggactatta ggacaaggcg gcttgtatgt agtgagcgag tatgtggatg gcctaccgtg
    10681 gtcacagttt attgctgaga acgtgttagt acaatcccaa cgttttacaa ttgcggaaaa
    10741 gttgatcaac accattcatg cttttcatga aaagcagtta cctcatggag atctttgccc
    10801 agagaaactg ctggtacaag tcggggagca gacagtaatt actctgattg gattgcttga
    10861 attcagtgat gaattaactg cagataatcg ctaccagcca gagaatcccg aaagtactga
    10921 tgcttttggg cgagattgct ttgcagtata tcgtatggtg gaggagctat ttagtgaaga
    10981 tatgccagta ctggtgcagg ctgagctaga acgcgcaaaa caaaccgttg acggtatacc
    11041 tatcgcgctc gatcctttgc tgcagtcaat tcgagcaccg gaacaagctg agattaatca
    11101 agttgtggcg tctgagtcac aggataaggt aattcctgtt tgctggggca cagatgattg
    11161 gccgcaagaa gtgaagcttc tagaacaaaa tgatgggatc tattattttc aatgtaactg
    11221 gtcatctaac ccacgctttg cgcatgaatt gcgttgttac atcactggcc taggagagcg
    11281 gctattgata gacttagatc ctgataatcg cactattaat agaatagtgt atgaaaaagg
    11341 attatcgatc gaagaaagta taaaggctgg taaatattcc caggctaaaa ttaatactca
    11401 actttcatta caacgtggct cacttaatca gcgtaatact tttattgaac tactgtttaa
    11461 cctcgagcca gtaattgatg ccatcattga gcgagctaat cctaatcaag agatggatga
    11521 agatgacttc gatagtagtg agtcaagccc aattgagtta tggcaggcat tatctgatac
    11581 agaagtagac ctacgagata tagtcaacat cgactctact gactttcagg aatcaccgag
    11641 tggttgctta ctctacccat atactacgga atccggtgct gacctcagct ttgaacttga
    11701 tgataagatc attgtttata ttaaagataa gcgtgaatca gtgcaattag gggaattgca
    11761 gctaagtgag actacgccga gtctattggc tattcgcttt gattttgatg ctgctcgtaa
    11821 gcgaattagt agcggcagcc agctacaatt ggaatcgatc cgtgacaaat catcaagaga
    11881 gttgcgtcaa agagcccttc aacgggtaat tgaaaacaaa gcagagatcc agcatctgcc
    11941 acagtatttt gattaccacc agaaaccctg catgcagcaa atgcaaccgc ggccatccgc
    12001 ggagacatta cgcgcacttt atgatcagcc tggacaacgt tttaatgaac agcagctaat
    12061 ggcatttcaa cagttggtcg agtttggacc agttggagtt ctgcagggac cacctggaac
    12121 aggtaaaaca acatttattt caaaatttat tcactatctg tatcaacatt gcggtgtgaa
    12181 taacattctt ttggtcgggc aatcccatgc ctctgttgat aatgtagcca tcaaggctcg
    12241 agagctctgc catacgaaag gaatggaact ggatacagta cgtattggta atgaacttat
    12301 gattgatgag ggtatgctaa gtgttgcaac taaagctctt cagcgacaga ttcagcataa
    12361 atttcaccgt gaatatgatc tgcgagttag ctccctagga aagcgcctag ggatggcccc
    12421 attattagtc caacagttat gtcagttaca tcgtacgctg aatcccttga tggtgacata
    12481 tggccaatat agccgtgagc tggataaagt agaacaaata aagagtagta gtattagtca
    12541 tcaagagcga ctggctgaat tattagaaca aagcaatcag cttaaactgc gaacacaaga
    12601 aattattaac tcaatattcg atgacagctt gctgaaaact cttgtctatg atgaaacctt
    12661 gataagacag ttggctgagc aagttgccat acaatacaat tataacaatc cagagaacct
    12721 tgaacgtttt atgcagctat tggaaatgag ccaagagtgg atggatgtat tacgcggcgg
    12781 cgaggctgga tttgatcgat ttatgttcaa aagtaagcga ttggtttgtg gaactcttgt
    12841 tggtgttggg aatcgtcgac tagaactagc tgagtccagc tttgattggg taatagttga
    12901 tgaggctggc cgagcacaag ctgctgaatt gatggtagcg ctgcaatcag gcaagcgggt
    12961 gctgttggta ggggatcata aacaattgcc accattctat catcaacagc atcttaagtt
    13021 agcctctaag aaattagaac tcgggaaagg gatcttttat gagtctgatt ttgaacgtgc
    13081 ttttaaagca acaggcggcg taacactcga tactcaatat cgaatggtag aaccaattgg
    13141 cgagttagta tcggagtgct tttacgctca agatatcggt aaactgcatt catcgaggaa
    13201 agtctcgcca gattggtatt ccaagttacc aatcccttgg aacaaaactg ttacttggat
    13261 cgatagttcg agccctaatg aagcaggtgc agaagaacat aagggtaatg gtcgttacta
    13321 taatcaacga gaagtccggc tactgctaga ggctttgcag tcattgtcga gtgatggctg
    13381 cattgcacag cttgagcaaa ctattaccac agaacagcca tatcctattg gtataatcac
    13441 aatgtatcgt cagcaaaaag aggaaattga caatgctatc agtcgggctg aatgggctgc
    13501 atcgttacgt ggtttgatca agatcgatac cgttgattca tatcagggcc aggaaaacaa
    13561 gataattatc ctcagtctgg ttcgcgataa tcccaacaaa ctacaaggtt tcctgcgcga
    13621 cgcgccgcga ataaacgttg ctatttcgcg agctcaagaa aggttattga ttctgggagc
    13681 aaggcgtatg tggtcaaaga ccaataatga ttcagcactt ggaaacgttc atgaatttat
    13741 tagtaaacag gttgcagtag atgaacccaa ctaccaaatc ctgtgtggtc aaagtctgct
    13801 tggagataac aactaatgtc agaaccacgt ctgggtaatc tgattaccgt tttactacct
    13861 gcgcgtagtt acaagatcaa ctgcgctttg accactgaaa aactgatgcc tggaattgaa
    13921 cagtttgcat gtcgcttgct gctgattttt gatcaactct atcccagcga gttacagaat
    13981 tactttggtc taactgatcg tgagcgagag gtattgcttg atgggttgct ggctaacaga
    14041 ctgatcaaca ttaatcctga tgggcatatt gaggctagct cattcctacg taagcatgca
    14101 gctaataatg gtgggaagcc aagtttagtt aaatatcaag aatgtacgga ggaagttgca
    14161 ttcgatctac taactctttc gatatgtaaa ccgcaaccaa atcgtcgttt tacttctgga
    14221 ctgccagagc tattgccgcg gcatcagatc gggggagatg ctgctgcggt aacagaggct
    14281 tttagttccc agtttcggca ccatcttttg ctcagccgca acagcgagta tgagcgtcaa
    14341 cggactaaat tatataagat aatgggctgt agttcgcatg agatggtgca gctcccaata
    14401 gagatagagg ttagctacgg tgtttctgct gggagcattg agccgcagaa atttactcgt
    14461 tcctatgaat atttaggtaa cacccggctg ccgctttcaa acgagctgga agctcatatc
    14521 gcagattttt tgggagaaca taaactagat gaattcggta tcgactgtga agatttctgt
    14581 aaactagcaa atgataaagt gttgttacaa tttgctaatg gttataagtt caactattcc
    14641 ggctggatag aggctcgtga acaacgtaaa actggctacg gtacttcatt gactaccggc
    14701 atgttagggg ctgtttattt gccgcacaat tctaagctgt tcattagtat gttgcataat
    14761 gcattacgtg attatatagg taaaacagct ccaaaagcgc tgtggtatag cagtaaagta
    14821 ccactgtggg gagctaatgg tagtcaactt tcgcgtttta ctcgcgctct aggcgatata
    14881 cttggcaatt atgccgatga taagattgct cgcatttcgc ttttacactc aagtgcagat
    14941 gaaggtgaaa aacgtcaaga gcgtaagcgg cacttaggtc gttttcctac cggtattggc
    15001 cttacttcag aggctaaatt tgatcgtttg gagatcctct taattcctga tgtgattgct
    15061 ttggtgcaat accacggtca acctaattct gatagtgcat taaccctgcc gattggttat
    15121 ataactgttg agccagagcg tttagaatta cttaaaaaac taatgattaa gcgaactgaa
    15181 ggggctgttg caaccattac ttggtctgaa tcaaaatttg aaaatttagc ttcgctatta
    15241 cctgttgagt ttctgattaa actgaataag aaaagcggtg aagatgtgga tgctgcaata
    15301 aaaaaaatgc agatctataa ccgtgctgaa accgcacggg caattttatc gctacgcaag
    15361 tagcatttat attgcaacga ataaattttt ctaggttgct atgaactagc taaagggcaa
    15421 caaatagata aacggcgtta ttcatgtcaa atgagataat gttaaattga tagggattta
    15481 taccccgccg gccattttga atggtcggag ttgttataaa cgttaaaccg ccccggaaat
    15541 cctggagact aaactccctg agaaagaggt aaacaggatg actaaaaata cacgtttttc
    15601 ccccgaagtc cgtcaacggg cagttcgtat ggttctggaa agtcagggcg aatatgactc
    15661 acaatggaca gcaatttgtt ctattgcccc taagattggc tgtacaccgg agactctgca
    15721 cgtctgggta ctccagcatg agcaggatat tgggggcggt gatggtggtt tcaccaccac
    15781 tgaacgtcag cgtctgaaag agctggaacg tgaaaatcgt gaactgagcc gcagtaacga
    15841 tatccttcgc caggcttcca cttattttgc gaaggcggag ttcgaccgcc cctggaaaaa
    15901 ataatgccac tgctggataa gtcgcgtgag cagtacggga tcggaccggt atgcagtgaa
    15961 ctgcatattg ccccgtcaac gtattaccac tgtcagcaac agcgacatca tcctgataaa
    16021 cgcaatgccc gtgcgcagcg tgatgactgg ctgaagaaaa agataccgtg cgtatacgat
    16081 gtgcgtaaag tctggcgtca gttgtttcgg gaaggtatca gagtggccag atgcactgtg
    16141 cacgtctcat ggcggttatg gggcttgtcg gtgttctctg gggtaaaagg tccgcactac
    16201 cgtcagccgg aaaaccgttg ccgcaggtga ccgcgtaaac cgtcagttcg tggcagaacg
    16261 tcctgaccag ctgtgggtgg ctgattttac ttacgtcagc acatggcagg gcttcgtcta
    16321 tgtggcgttc atcattgatg tgtttgccgg atacatcgtg gggtggcggg tatcatcgcc
    16381 catggaaacg acattcgtgc tggatgcgct ggagcaggcg ttgtgggccc gtcgtccgtc
    16441 cggcaccatc catcacagcg ataaaggctc tcagtatgtg tcactggcct atacggagcg
    16501 attaaaagaa gcaaaactgc tggcatcaac aggaagtaca ggtggcccgc atgacaacgc
    16561 gatggcggag agcatcaatg gtctttacaa agcggaggta atacaccgta agagctggaa
    16621 aaaccgtgca gaagtggaac tggtcacact cacgtgggtg gactggtata acaatcgatg
    16681 cttactggta aggctgggcc acatccctcc gtcagaggca gaaaaagcgt attatgcttc
    16741 catcggaaat gatgagctgg cagcctgagt tcacagataa aacactctcc aggatttccg
    16801 gggcggttca gttcaccaga aagtttcatc cattttcttc cacccaagtt gcggaagaac
    16861 cacttttcat ccgtcgttga caatcctgac ctgccccagt attagataca attatcagtt
    16921 agtaatgtcg gtttggttac cttcacattt tccatttcgc catagtgctg caagctctga
    16981 tggcgtctga taattcagtg ctgaatgtgg ccgacactcg ttataatcct gccgccagtc
    17041 attaatcact ttcctagcgt gaacgatatc gctgaaccag tgctcattca ggcattcatc
    17101 gcgaaatcgt ccgttaaagc tctcaataaa tccgttctgc gttggcttgc ccggctggat
    17161 taaacgcaac tcgacactat gctcaaaggc tcattgatcc aatgcgcggc agagctcacg
    17221 ggcagaaacc ccggctttag cttcgaggag gatactgatg atctgttcgt cggaaaaacg
    17281 cttcttcatg ggatgtcctc atgtagctta tgaagacatt actaacatcg cggtgtatta
    17341 atcaacaggg agcaggtcag tcccgcgagt ggttggacgt cagatgagtc atcaggaacg
    17401 taaaaaagta cgggctgctt atatccataa agtggaacat ttgaaagatg cagcgactga
    17461 tgttgcaatg gtgagtggat tttctggatg taagtcgcga gaaagggatt agcccgtttg
    17521 actatgcgaa agtctagtca aattagaagg cttttcgcaa agaagtacac ttttattggc
    17581 atttaaaata tactgtatat aattacagtt agtttatttc ataatcaatg cctggatacc
    17641 aagaaagtgg ctttaattag aaaaaaggtg gccatcgggg ctggtttccg ttaggatcct
    17701 tggttcaaaa agtgatgaaa aagccgatat gaaaaagaaa ccactgaaac agtacaaggt
    17761 atcctcgttt ttcgcaggga ttggtggatt cgatcttggt cttgagaaag cggggatgga
    17821 agttgttttc caatgcgaaa tcaacaaatt ttgccagaaa gtacttagaa agaattggtc
    17881 aaaagttcca ctacatactg atattacacg acttaatgct gatgagatcc cagagagcaa
    17941 cgtttggtgt ggtggttttc cttgccagga tgtgtcgtca gctaaccaag gaaagcggaa
    18001 aggtctagaa ggggcccgaa gcggcttatt ttatacatat gcaaaactca tcgaagaaag
    18061 aaagcctgaa tggcttatta ttgaaaatgt tcccggactg ctcaacagcc acaatggaca
    18121 agacttcaaa gttgtcatcg atacgttggt cgaattcggg tatggcgttt cgtggagagt
    18181 attggatgca aaatactttg gaacacccca gcgtagaaga agagtgtaca ttgtcgcaag
    18241 tcttggagac atgcgctccg ctcgagtcct ttttgaaccc ggagcaacta gaatcgttga
    18301 taaacagggc caaggagaga gggcaaatgc ttccggagcc tctgctacag gcctatcaaa
    18361 agcagatatc tattctatcc agcatgcaag tattggacga aaagcaaccg caggacctca
    18421 agcaaaagga tacaggaacg atggagaaac ctacactctt gactcaagag gaagttcaga
    18481 tgctgtatgt gcgccggatg ctgccttccg agtacgagcg tctacaggga tttcccgaga
    18541 actggactct aatcgattca gagcagtagg gaacgctgtc gcggtcccta ttgttgaatg
    18601 gttaggaaaa agaattgtcc ttgtagatca gcaggtgctc aacgaggcat tatcgtaaca
    18661 gaactattat ctaaacgtaa aaatgatcct tcgaatagtt cagggcgtgg tgcgccctga
    18721 tctgttagaa atcttacaaa tgttgaataa ttggtgaata gtgtttttga gtcatcaatg
    18781 attaacggga tcggccctgg acgtggttca tccgatgtat cttccttgaa agctaaaata
    18841 cataatgtcc agttatgggc tttcagatct ctgagatttg cattcctaaa acgtctgtgt
    18901 aaatagtcat agagtaagct accgacagca aaaacgattt tccctccggt ttgatcgaca
    18961 acattccctt tgacaagaaa ttgttcttgc tgtcgtctcc atgcgtttgt ttcgatagta
    19021 tttgctgcta caggttgtct tagaatttca ttagtaggaa actcaagatc agcccatgca
    19081 gcgatatgtc gagtaatttc tccagtgcta cttgtttctc caccaccttg catctcaagc
    19141 aggacctcat caactcttcc cctacccgaa aaatttctca ttacatagtc tgcatggtag
    19201 ctattatcag aatctggtaa aggaatattg acttctcttc ttacaccaat ttctgaatcc
    19261 tgtaattcag gatcctgata aatgagtttt gctactttcc gtaatatatc tctttgatgt
    19321 tcaaccaaaa tattttcaat ttgtttttta cgaccattta caatgactgt ctttttctgg
    19381 cgagtagaac atagccgatg ttcgcaaact atccaaacag ttccatctgg tttgcggaca
    19441 ctgcatacag gaggtgtagg atttttgacc ttacttgctt tgtcacaggt tcgatccata
    19501 aaagggcatt gtatttgcgt accttttgca acgggaacaa tctgttggtc tacatctgtt
    19561 ctttgaccta aatattccgc gatactcact gccattgctc attcctcagt caagaatctt
    19621 atacgatatg atcatagtga gtggtaattt atgtcaatta attcatagaa cttctatatc
    19681 ttaggtgtaa gttttaaatg aaaatgaagg aagtccgagt cttgacgctg ctcatctctc
    19741 gatgcactca ttatttccca agtagactgg ccccctgaat ctccagacaa ccaatatcac
    19801 ttatttaagt gatagtctta atactagttt ttagactagt cattggagag cagatgattg
    19861 atgtcttagg accggagaaa cgcagacggc gtactacaca ggaaaagatc gctattgttc
    19921 agcagagctt tgaaccggga atgacggtct cccttgttgc ccggcaacac ggtgtggcag
    19981 ccagccagtt atttctctgg cgcaagcaat accaggaggg aagtcttact gctgtggccg
    20041 caggagaaca ggtcgttcct gcctctgaac ttgctgctgc catgaagcag attaaagaac
    20101 tccagcgcct gctcggcaaa aaaacgatgg aaaatgaact ccttaaagaa gccgttgaat
    20161 atgggcgtgc aaaaaagtgg atagcgcacg cgcccttatt gcccggggat ggggagtaag
    20221 cttagtcagc cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc tcagacgaac
    20281 cgatgactgg aaagatggtc gccgcagccg tcactcagat gatacggatg tgcttctccg
    20341 tatacaccat gttatcggag agctgcccac atatggttat cgtcggatat gggcgctgct
    20401 tcgcagacag gccgaacttg atggtatgcc tgcgatcaat gccaaacgta tttaccggat
    20461 catgcgccag aatgcgctgt tgcttgagcg aaaaaccgct gtaccgccat cgaaacgggc
    20521 acatactggc aaagtggccg tgaaagaaag caatcaacga tggtgctctg acgggttcga
    20581 gttccgctgt gataacggag aaaaactgcg agtcacgttc gcgctggact gctgtgatcg
    20641 tgaggcactg cactgggcgg tcactacggg cggcttcgac agtgaaacag tacaggacgt
    20701 aatgctggga gcggtggaac gccgcttcgg caacgagctt ccggcgtctc cagtagagtg
    20761 gctgacggat aatggttcat gctaccgggc taatgaaaca cggcagtttg cccggatgtt
    20821 ggggcttgaa ccgaagaaca cggcggtgcg gagtccggag agtaacggca tagcagagag
    20881 cttcgtgaaa acgataaagc gtgattacat cagtatcatg cccaaaccag acgggttaac
    20941 ggcagcaaag aaccttgcag aggcgttcga gcattataac gaatggcatc cgcatagtgc
    21001 actgggttat cgctcgccac gggaatatct acggcagcgg gccagtaatg ggttaagtga
    21061 tgacaggtgt ctggaaatat aggggcaaat ccatttcgca agcaaagact ctggtaaata
    21121 ctcgtcatat ttatccagat ccttatcagg aacaactgta gcaggctcaa attctgaagg
    21181 tgcaaaatgg catctgaatc gatcacgact tggcatgctt ccgatcattg attggcatcc
    21241 tgccagtcgt atcgtaagcg taaaccgacc gccgtatgta gccattagac aagaattggt
    21301 aacttagacg cccatctgac acagacggac atctaagtat ggaattgcag gactggcgaa
    21361 aagaacctcg taaaaagtat tcgaatgaat tcaaacttcg tatggttgaa ctggcatcac
    21421 aacccggtgc ttgtgtggtc cagattgcac gagaaaatgg cgtcaatgat aatgttatat
    21481 tcaaatggct cagactctgg caaaacgaag ggtgtatatc gcagcgtctt ccggtgacaa
    21541 ccacttctga cgcaggcgtt gaattattac ctgtagagat aacgcctgat gagccgaaag
    21601 aaccggtggc tgctcttact ccgtctttat ccacccagac tactgtcagt gccagctcct
    21661 gcaaggtgga gttccgtcac ggtaacatga cgctggaaaa tccttcacca gagctgctca
    21721 ctgtgttgat ccgtgaactg accgggaggg gacgatgatc tcactcccgt caggcacccg
    21781 catctggctc gttgccggcg ttaccgatat gcgtaaatcc ttcaacggac tgggagaaca
    21841 ggtacagcat gtgctgaatg ataatccctt ctccggtcac ctgttcatct tccgtggccg
    21901 acggggtgac acgattaaaa tcctgtgggc tgatgctgat ggtctgtgcc tgttcaccaa
    21961 acgcctggag gaaggccagt ttatctggcc tgcggtgcgt gacggcaaga tatccattac
    22021 ccgctcgcaa ctggcaatgc tcctcgataa gctggactgg cgtcagccaa aaacatcccg
    22081 tcttaacgca ctgacaatgt tgtaaaaatg tcatggccgg attataaaaa cggtcatgag
    22141 tcagaaatac ctcattcgga tcactgagct ggaaaggttg ctctctgagc aggctgaagc
    22201 cctccgtcag aaagaccaac aactgagtct ggttgaagag acggaggcct tcctgcgttc
    22261 tgcgctggca cgtgccgaag aaaagatcga agaagatgag cgggaaatag agcatctgcg
    22321 ggcccaggtg gaaaaactgc gccggatgct gttcggtacc cgttctaaaa aactgcgtcg
    22381 tgaagttgaa caggctgaag ccctgctgaa acgacgcgaa cagaacagcg atcgttacag
    22441 cgggcgtgag gatgacccgc aggttccccg ccagttgcga cagtcgcgtc atcgtcgccc
    22501 gttaccggcg catctgcccc gcgaaataaa ccgcctggag ccagaagaaa gctgttgccc
    22561 ggagtgtggt ggtgagctgg attatctggg ggaagtcagc gcagaacaac tggaactggt
    22621 gagcagcgcc ctgaaagtga tccgcacaga acgggtaaaa aaagcctgta caaaatgtga
    22681 ctgcatcgtt gaagcaccgg caccgtcccg tccgatagcg cgtggtgtcg cgggcccggg
    22741 gttacttgcc cgcgtgttaa cgggaaaata ctgcgaacac ctgccactgt atcgtcagag
    22801 tgaaatcttt gcccgtcagg gtgtcgaact gagccgtgcg ttactctcca actgggttga
    22861 cgagctcctg ccatggaacg tagcactcac caataaataa gtatcaatac ggcactccgt
    22921 taacgcttac tgaaacgacg ccggggacca tcttcttttg ggtaaaaaag gccgcgccag
    22981 aattcgacac gaccactgct atatgcgtat tattctgttc cgggagattg cctctcttct
    23041 ggttattcag caatacaacg gacaaaaagc actggcataa ataacccctt gttaaacgga
    23101 gaatagtgat aatgtattga cgctattact catttctcat cttcatcctt cgggtaagca
    23161 gagtcgcaca aatgaatccc agcagactaa atgccaccat tacagaaaaa acatagttat
    23221 agccctgtat gccagaaaaa ttatctaaca gcgaaccata aagcgtgtaa gcaaacatgg
    23281 aaggcatgta accaataatg cacccaaacg ccattgctga accagcaaat tctctggggg
    23341 taccgatttc atccattggc gcaaaaaaaa ttgcccgttg tgaaaaaatg attgcaccaa
    23401 atccaagagt tgcagtcatt ccaagataaa cattcattga gtcgtgtggt agttgaataa
    23461 aaagtagcat agcgactgct gaaataagga aagtccattt cagatagatg accggagaat
    23521 gagaaatttt atctgcgaga taaccgccaa ctggcccgcc caccattttt agtccgtact
    23581 ggttaataat cccgtaagca cccacgagtg caacgggaag ggcatagata tctttcagga
    23641 atggtatgaa atatgttagc ccacaatatg cggagtacac aaagaatata caaaatgagg
    23701 ccagccacag atttggtgat ttaacgacat tctttatccc cttgaatatc tgtttattaa
    23761 catcttcgtc attctgtgga atggtcctgt cgtctgcatc cacaataaag taggtgataa
    23821 ttccaacaat aatggtgatt aatgtgtaat aaacaagacc agcctgcatg ccagcttttc
    23881 cttcgccaaa ctgcacaaag acgaataaag cgccggacgc aattaataca tccacgactc
    23941 ctcgtccagc ttccagaaag ccgaacatac gtccctgttc atccctgttc cccaacagac
    24001 gaacagcttt aagtagcacg ggccagtaga ccacttcgcc aaagaaagcc atagcaccaa
    24061 aagctataag gtaaccagag aaggggggta atgttgtgag gtaagcgccg cacagaccca
    24121 caccgattaa acctgcagga agaagtattt ttttggaaaa gcgatctgct atatataatg
    24181 aactaaatag tccgatagtc tgaacagcag catatattgt aaatccaaag ccaatctgtg
    24241 tattagtcaa tccccagtca gcctgcatag gaacataaaa tacgtccttc atactggaca
    24301 atttgaaaat tgtcccaccg ccaagaataa ggaagcatag cttcatccat ttattcatct
    24361 ggttatcccc cattatttga taaccgcaat ggcttcaatt tctatcagca cgtctttcgg
    24421 taatctggcg acttcaacac aggacctggc cgggtatttg tctgatgaga aatattcact
    24481 gtacagaagg ttaaattgag aaaaattatc catgttactg ataaaacagg ttgtttttac
    24541 gatgttatcc attccgattc ctgcagattc aataattgcc ttcagattat ccatcgcttg
    24601 cctggtttgc ctttctatag tctcgccatt tacggttccg tcttctggtt tgaaaggaca
    24661 acatccggaa atgaacaata aattaccagt tgatactgcc tgtacatagg gaccgatagc
    24721 tttgggggca tgtttagtat caatgatcat aaaagtatcc ttttaaattt attaacaata
    24781 tttttttctt aaattcttca gagtctgaac agaaacctca acacaacgat tttgggctgc
    24841 cattagttcc tgtaatacag agtcagatat tttggcaggg tagtaataat caagtggctt
    24901 tattttcagg tgttcaaatg gtgcctcttt tacggagaat gccccttcca gagaatatac
    24961 gccgccagta ccctttggac gggcaaaagg tgaggcatat gggaaacatg tttcgatgtt
    25021 tattctggtt aaagaggact ctttaaccag agttttaaag caggtatcaa tatcaatgct
    25081 tccttctccg acaggaacac cacagacaac caggtcttcg ccgttgcgtg tgacaatatg
    25141 atccttgaaa tgcgtgctgt aagcataagg agccagttta cgaactgttt ctactggttc
    25201 ttcccatgcc atcattccat tacctatatc acagtgtgct cccacccact cagaattaac
    25261 agatttgata acttcgatta tctcgtcggc agtttcatct tcatggtttt ctattgccag
    25321 tttaattttc tttctactga gtaaggatgt cagtgatttt aactgttcta ctatttccgg
    25381 atatttcaga gggttatatt ctcctctcct gaacatataa gttcggataa cttctgctcc
    25441 aagtgcagat gcaatttcca ctgctctggt gatagcttca caggaagtga atcgggtatc
    25501 tatctcacaa taaagaccat gagaatcaat ggattttctg acttttgaca gatattcagg
    25561 agatatactg ttcaggacac caaattccgg atgcagacca acatccggaa tgatattaat
    25621 ttcgacccca tccagcccca gttcactggt tttatcaatg aagtcgaaaa tatccatcat
    25681 tccctgctga aagaacagat gatatgactc agtctcaagg cctagtttca tggcgaacct
    25741 ccatagtatt tgagactgat tgtagttttt tatacatttg taaaatgtga tcaaagtaga
    25801 ataatttctc acttagtgaa taaatttact acttggtgtt attttgagcc actttggtgg
    25861 actctgttct gcatctggta tagcattcag atgtactttg ttcatgaggt tattatatga
    25921 aatctattgt taatcatgag tttactgagg atgacaggca gcttttgctc tcatatattc
    25981 cattcatcga cagtcttggt cagttcttgg gtgagtattg cgagatcgtc ctgcatagtt
    26041 ttgaggactt gcatcattcc gttattcata tcgttaacgg gcatgtgacg gggagagaga
    26101 agggggcacc tgtaacaaat atagcactgg aaaaactggc agaatttaac tcaacaaacg
    26161 atatgtggga tgtctatttc aaccataaag gcgttcgccc ttttaaatca tcgtcatcac
    26221 ttattctgaa taaaaataaa gaaccagtag gtatgatatg tatgaacttt gctctggata
    26281 tgcctcttaa tgcatttatt gaaaactttg caaaatcgtc aaactgtaaa aacgaaaatt
    26341 tcactcagga tatcaataat cttgtgctta gtcaccttga acctgttaaa aatatggttt
    26401 actccaataa gaatatacca agtaaaaaca agaactttga aatagtaaaa gaacttaatt
    26461 ctattgggtt gtttaagttc ccggttacaa caaaaattgt ttcatcggtt cttggtatca
    26521 gtgctaacac tatttataaa catctcaggg tattaaataa taaggggaag gagaattaag
    26581 gtgtctgtat tctgaactta taagtaagtt gtttgtctga agcgtattga tatttatttt
    26641 tcggttagag caactttcta gggaaacaga tcgcgtatcc agtttgcctg tcattcctag
    26701 atatgttcaa tgacgtaacg cagccatttt tctggagcca tattgttcag acggcatgta
    26761 ccaatccgca ggtacagcat cgccgcatcc tcaagtaatg ccggtaattt ggggcgtaat
    26821 tctgttgcgc ttttactgaa cacaatgccg tattcagtaa gaagtccaag aacatgatct
    26881 atcagttgtg tccttccatc gagatcaacc gatgcatttt caaaaaagcc tggacgttct
    26941 gtaggtccag actttttatt ggtaccagtc gtatagttct atattgatag gtttcagcta
    27001 ttgctgtgcg ttgttataat catttttctg acgccattga taagatttaa catgttgtgc
    27061 tggatgcaat attacatcat agttgagttc ccttatttcc tgtgggcagt gatgggatgt
    27121 cccgcaagcc ttcatcgcaa cggtcatttt ggctgttata ccagccagtt aagcagttgg
    27181 ttgtgagaaa gtttgatctt tcccgcgtga ttgccacgag cattgatact gaaaagataa
    27241 aagttcgttt tagcctgagc gatgtcggta attttctgtt caatcatggc ctttctctta
    27301 cttttgtggt taaaatttat tcgctatggc gcaattgaaa gcggtcgtga aggtggggga
    27361 agactatctc atcacttacc atatttccga catattctca caacaaaata ccacgtaact
    27421 attgatgaat attgttctct cctgagcagc gtttatgctt taattgctaa atcgatttaa
    27481 ttagtttagc aattagtata ttggagcctt tatgagcaca agagtatggg tacttggtga
    27541 tgcggttgtt gatttattac ccgaaagcca ggggagacta ctacagtgtc ctggcggggc
    27601 gcctgctaat gttgcagtcg gtatcgcaag gctggggggg aaaagtgcct ttattggcaa
    27661 agttggcgat gatcctttcg gtcgctttat gtatcagaca ctgagtacag aaaatgttga
    27721 tacacgttat atgtctcttg atcctcaaca tcgcacctca attgtggctg taggacttga
    27781 tgagcaagga gaaagaaact ttacctttat ggtacgccca agtgccgatc tttttttaca
    27841 acctgatggc cttcctgcat ttgggccggg tgaatggctc catctttgtt ccattgcgct
    27901 cagtgcagaa ccttcccgaa gtaccgcatt tctggctatg gagaaaatac gtcaggctgg
    27961 cggaaacatc agttttgatc ccaatatccg cagcgatctc tggcagagtg aagcgctatt
    28021 aaggaaatac cttgatcgcg cactttcgct ggcgaatatc gctaaattgt ccgaagaaga
    28081 gttgctattc atcagtggcg aaagccaggt tcagcaaggc gcatattcat tagtacaacg
    28141 ttattcgttg actttattgc ttatcacaca aggaaaaaat ggcgtacttg tgtattttca
    28201 ggggcagttt atccactatc ccgccaaacc tgtttctgtc gtcgatacga ccggggcagg
    28261 agatgctttt gtcgctggat tacttgccgg tctggctgat tctggaatac caacaaatac
    28321 cagacagctt gaacgaatca ttgtacaagc tcagatttgt ggtgctctgg cgaccacggc
    28381 taaaggcgcg atgaccgcct taccccgaca acacgatctc ccttcacaat agtgatcctg
    28441 ctgttgtatc tgtaacccga tttgtcgtgc ctgtcacatt tactaaatcg gtttagcgaa
    28501 aataattgct ttggctaaaa agtcagcttt attatcaaca cctaaaccgg tttagcattt
    28561 tagcaatttt tataattctc tgatgggacg cgacaaatta tgtataaaaa aacaactttg
    28621 gcagtgttaa ttgctttgct gaccggtgct acaacggtac atgcgcaaac ggatattagc
    28681 agtatcgaat ctcgactggc ggcgttggaa caacgtttaa aaaatgcgga atcccgcgcc
    28741 caggcggcag aagcaagggc caaaacagct gaattacagg ttcagaaact ggctgaaaca
    28801 caacaacaaa atcagctaac aactcaagaa gtagcacaga gaacagttca gctcgaacag
    28861 aaatccgcag aaaacagtgg ttttgagttt catggctatg cccgttccgg gttactgatg
    28921 aatgatgccg cttccagtag caaaagtggg ccgtatctga ctcccgcagg tgaaactggt
    28981 ggagctgttg gccgtctggg aaatgaagcc gatacctatg tcgagttaaa tgtagaacat
    29041 aaacaaacac tggataacgg tgcgaccaca cgctttaaag caatgttggc tgacggacaa
    29101 agagattaca acgactggac tggcggctcc agtaacctga atatccgaca ggcttttgcc
    29161 gaactgggcg cattaccaag ttttaccgga gcattcaaag acagtactgt ctgggctggt
    29221 aaacgctttg atcgcgacaa ttttgatatt cactggttag actccgatgt cgtattttta
    29281 gcgggaacgg gcggcggtat ctatgacgta aaatggaacg atacatttcg cagtaacttt
    29341 tctctctacg gacgtaattt cggcgatctt gatgatatcg acaataacgt tcagaattac
    29401 atcctcacca tgaatcatta tgcaggcccc ttccagttga tggttagcgg attacgggca
    29461 aaagataatg atgatcgaaa agatgccaat ggtgatctca ttcaaactga tgctgcaaat
    29521 actggcgtac atgcgttagt tggtctgcac aatgacactt tctatggcct gcgtgaaggg
    29581 acggcaaaaa cagcactgct atatggccat ggcctgggtg cggaagtcaa agggattggc
    29641 tccgatggcg ccctgctgtc tgaggcgaat acctggcgct tcgcatctta cggcacaaca
    29701 cctctgggaa gcggttggta tgtcgcacca gcaattctcg cacaaagcag taaagatcgt
    29761 tacgtcaaag gcgatagcta cgaatgggtg accttcaata cacgtctgat caaagaggta
    29821 acacagaatt ttgctctggc ctttgagggt agctatcaat atatggatct gaaaccaaag
    29881 gggtatcaaa accacaacgc cgtaaacggc agtttctata aactcacctt tgctccaact
    29941 ctaaaagcta acgatatcaa taatttcttt agccgtccgg agcttcgcct gtttgccacc
    30001 tggatggact ggagcagcaa acttgatgat tttgccagca atgacgcttt cggcagcagt
    30061 ggtttcaata ctggcggaga gtggaatttt ggtgtccaaa tggaaacctg gttttaatac
    30121 cagacaacct cacagatgtg aagtttttac tgaaataaca atgaattaga gggttatatg
    30181 gatttcgata aaatcgccca atcgttgctt cctttattag gtggtaagga gaacatcgcc
    30241 agtgcagcac attgcgccac tcgcctacgg ctggtactgg ttgacgacac acttgccgat
    30301 caacatgcca ttggccagat tgatggagtg aaaggttgtt ttcgtaattc agggcaaatg
    30361 caaattatct ttggcaccgg cgtggttaat aaagtctatg ctgcctttat tcaggttgca
    30421 ggtattagcg aatccagtaa agcagatatc gctcgactcg ccgctcaaaa actcaatcct
    30481 tttcagcgaa tagcacggct actttctaat atttttgtcc ccatcattcc agccattgtt
    30541 gcttctggtt tattaatggg gcttctggga atggtgaaaa catatggctg ggtgaatacc
    30601 gataatgcga tttatatcct gctggatatg tgcagttcag ccgcatttat tattctcccc
    30661 atccttattg gctttactgc tgctcgtgaa tttggtggaa atccttatct tggcgcgaca
    30721 ttaggaggga ttctgactca cccggcactc acaaatgcct ggggcgttgc tgcgggattt
    30781 cagacaatga acttcttcgg ttttgaaatc gccatgattg gctatcaggg aacggttttc
    30841 cccgtgcttc tggcagtatg gtttatgagc atagtggaaa aacagttacg caggtttatc
    30901 cctgatgctc tggatctcat tctgacgcca tttctgactg tcgtcatttc tggctttatc
    30961 gctcttttga ttattggtcc ggcaggacga gcgttaggtg acggtatctc tttcgttctc
    31021 agcacgctga ttgcacacgc aggctggtta gcagggttgt tattcggcgg gctatattca
    31081 gcgattgtta ttacgggtat tcatcatagc ttccacgcaa ttgaagcagg acttttaggt
    31141 aaccctgcaa tcggtgttaa tttcctgctg cctatttggg caatggcaaa cgttgcgcaa
    31201 ggcggtgcat gtctggcggt atggtttaaa accaaagata caaaaattaa agcaatcacc
    31261 ctaccctcgg ccttttccgc aatgttaggg attactgaag ccgctatttt tggtatcaac
    31321 cttcgttttg ttaaaccttt tattgcggcc ttaattggtg gcgctgccgg cggtgcctgg
    31381 gttgtctctg ttcacgttta tatgactgcc gttggtctga ccgcaatacc cggcatggca
    31441 atcgttcagc caacatcgtt ggttaattat attattggaa tggtgattgc tttcgctgtc
    31501 gcttttagcc tctctttatt attcaaatac aaaacggacg aggagtaatg tcgatgcctt
    31561 cacaaatgcc cgcgatttta caggcagtga tgaaaggtca gtccaaagca ctggcagatg
    31621 cccactatcc ttgttggcat ctcgctcccg tcaccggact aatgaacgat ccaaatggct
    31681 tttgctggtc tggaggccgc tatcatctgt tttatcagtg gaatccactg gcttgtgatc
    31741 ataagtacaa atgttggggg cactggagtt cgacggattt attgcactgg cagcatgaac
    31801 cattagcgct aatgcccgat aaagagtacg accgtaatgg ttgttactca ggaagcgcgg
    31861 ttaataatca gggcgttctc acactttgct ataccggtaa tgttaagttt gatgatggca
    31921 gcagaacggc atggcaatgt ctggccaccg aaaataacca gggcgggttt gataaactag
    31981 gccctgtcat atcgttacct gatggttaca ccggacatgt tcgtgatcca aaagtctgga
    32041 agcacaacag ccaatggtac atggtgcttg gcgcgcaaga taaagagaaa cgggggaaag
    32101 tgctgctgta ttcctctgtc gatctgaata cctggtcttt tcatggagaa attgctggta
    32161 acggtctgaa tgaaatagat aacgcaggat acatgtggga atgtcctgat cttttcgccc
    32221 ttgatggtga atacattctc ctctgttgcc cccagggaat ggtgcgcgaa catgagcgct
    32281 atctgaatac ttatccctgc gcctggctac atggacagtt tgattacgag acaggcaaat
    32341 ttacgcatgg cgctttctca gagctggatg cgggatttga attctatgca ccacagacga
    32401 tggaagcccc ggacgggcga cgattgctgg tcggctggat gggggttcct gatggcgaag
    32461 agatgttgca gccaaccaga aagcatcatt ggcagcatca aatgacctgt tttcgcgaac
    32521 tctcattcca gaagggaaaa ttgtttcaga tgccaatcag ggaactcgcg caactgcgtg
    32581 aagctgaaca cttctggcaa ggaaaagcgg atcatgcgcc gcatgtcgaa attgaacgcc
    32641 tggaaatgga catcattccg tcgggtgagc tgtatctgaa ttttggcaat gcgctggcac
    32701 tgcatcttaa tgacgatggt attcaactac agaggagaag ccttgcaggg caagagaaac
    32761 taactcgtta ctggcgagga agcgtaacgt cactgaagat tctctgtgac agttccagcg
    32821 tagagatatt catcaataat ggtgaaggtg taatgagtaa tcgttatttt ccccatcatc
    32881 cagcttcgct gatattacag ggtgagtctg acgtcacgtt acgctactgg tcgctacgcg
    32941 cctgcatggt agaatgaggg ttttgaatta cggatcctcg tcgtgagaaa aaccaaacgc
    33001 gttactatta aagacattgc tgaactggcg ggagtgtcga aagcaaccgc cagtctggtt
    33061 ctcaatggtc gtggcaaaga gttacgtgtt gcgcaggaaa cgcgtgagcg tgtgctgagt
    33121 attgcccaac agcagcatta tcagcccagc attcatgccc gctcattacg ggacaaccgc
    33181 agtcatacca ttggtctcgt cgttcctgaa atcactaact acggatttgc agtattttcc
    33241 catgaactgg aaacattgtg tcgggaagcg ggcgtccaac tcctcatatc ctgtactgat
    33301 gaaaatcctt cacaggaaac aatggtggtg aacaatatga ttgcccgcca ggttgatggg
    33361 ttaatcgtcg catccagtat gctgcatgat aatgactatc aaaaacttag cgagcaatta
    33421 cctgttgtgt tgttcgatcg ccacatgaac ggcagcacat tgccattggt cattactgac
    33481 tctgtttctc ccaccgccgc tctggtggca gatattgcac ggagtcaccc ggatgaattc
    33541 tattttctgg ggggacaacc ccgtttgtca ccgactcgcg atcgactgga aggatttaca
    33601 caaggcttgc agcaagccgg agtaacgcta caacctgaat ggattattca cggtaattat
    33661 catccgagct caggctatga aatgtttgcc gcgctatgcg ctcgtttagg acgtcctcct
    33721 aaagccttat tcactgccgc ttgtggatta ctggaaggcg ttctgcgcta tatgagccaa
    33781 tataatttac tcgacagtac aatccacctg gcgagttttg atgatcatta tttgtatgat
    33841 tcactttcgg taagaatcga cacaatacaa caggataatc gccaacttgc ttttcactgc
    33901 tttgagctta tttcgcaact gattgaaggt gaaacgccat ccccattaca gcgttatctt
    33961 cctgccagtt tacaaaaacg ctatagataa tttattaatt atcttatgta gctgagtatt
    34021 aggtgacaaa tgatcagtct ttcccacgac aattgtatcg ttctgatgaa ctcatattga
    34081 cgcatttttc gggcgtatca gcccccggtg ttacgaatgg tgcatacgaa cgatcgcagt
    34141 ttgacgatgg ttgatttatt ttgttgcgca ggggcaatcg cctgctaggt accgaaggct
    34201 ggctctgaag tactgagtcg ggtgatcgcg tctggccgct ggctgcgaat tactgacacg
    34261 ggattggtgg caggtgatga aatcctgtcc cggaaatggc tccggattga ttcaagttct
    34321 gacccgctgt cagcccgcgt ggttgctgct ctggctcatt tcctgtcgtc agttttctca
    34381 tgcccggctg cgggttttcg gactgaatga taatatctga cgacaggagt cgctcatcta
    34441 tcgaaggtaa atctccctga aagtaatagt tctggcaggg ggaacagaac tgtaatcatt
    34501 gtgccgtaat ttgagttggc tacctttgct acatgaaaac tttattcact ttgttcatgt
    34561 tgattgggtt tttcaatgag gaaagtttcc agggtacaat ctccattaac ctgtttccgc
    34621 aaagcaagtg gcattttacc gttaccagac cacgtcctgg tttcgccgtt ctcatcctca
    34681 aagatatatt tcgcttttct cacaccgcct ttgggttttc gtttggtttc cgttgtccct
    34741 gcaatagcag aaactggacc ctgccattct tccgggtcaa gccccacaga aacgatgtac
    34801 tcaaatacct gttgacattt agtttccagt tccttacgtt cagccgcttt tcgttcagca
    34861 tcttcacggc gttcttcaat aacagtgttg agcttttcct gcatgtcgag taattgctca
    34921 aaatcaagtt cgcgtacata cgtgcgtagt gaacgaatat tcgtcattgt ccgactgatg
    34981 atgctgtatt cttcttcttt agtcagtttg cacataattc ccctctcttt tgcaataaac
    35041 gtgttttctc tggtgggttg attacccagt cttttttact gtgtctgaca ttttgtactg
    35101 tggtgcagtt gtaatgcaaa ccttccgggc ttctgggaca cagcatttca gtccgggccc
    35161 catcattacc attttgttga ttcgaaacga aaaccctcac caatatcgat cgattttacc
    35221 gatcgatatg atcacatcga tcggttgtaa ccattttacc ctgtcttttg catggtttta
    35281 aaataggcaa ccctctttgt cgggtagggc aagaaccaac ggagatgatt gaaatgaaaa
    35341 aggttattgc ggtttcagcg cttgcaatgg caggcatgtt ttcggcccag actctggctg
    35401 atgagagcaa aacaggcttt tatgtgaccg gtaaagcagg tgcttctgtt atgtcacttt
    35461 cagagcaacg tttcgttgat ggtgaagggg catgggcgga taaatataaa ggcggtgaca
    35521 aaagtgatac ggtttttggc gcaggccttg cggtgggata cgatttttat cagcactaca
    35581 atgtgccggt gcgtactgaa gtggagtttt atggccgcgg taacgctgaa tcaaaatatc
    35641 gtctgagtta ttgggaaagt gtcggtggtg ccgagtttga tgatgcacag aacaagctca
    35701 gtgtaaacac gctgatgctg aatgcgtatt atgatttcag aaacagcagt gcattcacgc
    35761 catggatatc tgctggtctg ggttatgctc gggttcatca taaaacttcg tacatatata
    35821 ccgataacag tccggcaggc agtgaagtat attcagcttc agcgtcaaaa tacgaaaata
    35881 acctggcatg gagccttggt gtcggtgtta aatacgatgt gacgcaggat ttcagccttg
    35941 acctcagtta ccggtatctg gatgcagggg attccactct gacttataaa gatgaggatg
    36001 gcgctaaata taaatcctct gttgatgtca gaagtaatga gtttatgtta ggtgcaacat
    36061 ataacttctg atggccattt ctgcctgcgc tttggagcac agtaaagtgc aggtatgaca
    36121 aatcaataac agatatcctg atgatattct ccctctggca actaataatt tcagatgaat
    36181 gaaatataaa tagagcaaaa atattactct cgttgatttt ttacaggggg caggtagcgc
    36241 gcagtatggt tgtcagtctg ttaaatgaaa aaggactgtt gttgggacac gttgtttttt
    36301 gtcatatcag cccttccgga aacaataaac cgtattacga aggaggctcc cggacatctg
    36361 gtttaaagtt tgaacataat caaataaaaa tctgttgccg gagggcgtag ttttgtggaa
    36421 ctgacaaatg atgtcaggag tatttatata attcacttac acaagagtaa tactacaggt
    36481 gcagtgattt atggctgact caacgagaaa tcgctcagcg gagcgtttga ttgatatcct
    36541 tgttgaatta cagaattatg gcgtggtcag tcgtcataat ttgatgaaaa aatacaacat
    36601 cactgagcga acagcatatc gtgatctgaa tatgctttct ccctttattg aagcctgtgg
    36661 cgatggaaaa taccggctta tttcagcaag agcaggaaac cagagcaaag gatcactgca
    36721 caaatctctg gccaggttac tggatactga tgcgattttt cctgaacggg atgaaggttt
    36781 ctggcagaaa ttagaaaaca gggcaaccga aaagcatatt cgtgttcagt ttcataaccc
    36841 tgagcacact atcagggatg atttaagaaa atatcttgat gttcttgaaa aagcgatccg
    36901 caacagtaat atttgtcaga ttgcatatgc cggtaagacc agaatcgttc atccctataa
    36961 attaactaat cagagaagta tatggtattt gctggctact gaagagaaca agttaaagtc
    37021 gttttcatta gctaaaataa aatggctgga tataaaaaaa gaaaagtttg caaagagtga
    37081 ggaaatacag tcactgataa gtgagtcatg tgacccgtgg gtgagcgaca aaacatttga
    37141 tgtagtgttg ttcattcaca gtaatatcgc ccattatttt ttgcgacggg acttacttcc
    37201 ttaccagcaa ttgctacaca gacatgataa tggcattacg ctgtcatgca aggctgcaca
    37261 taaaaatcaa attatcccgt tgattttata ctggctaccg aatgttgaaa ttattgaacc
    37321 agtatggttg aaagaagccg tgttaacaat gttaggaaaa tacctcaccg ctgagaatgt
    37381 ttcctgaaat atttcagttg actgatgata aagcaacctg tacaggagga aaagaatggc
    37441 gttacctttc attattttgg gtgtggctgc cggcgctgcg gcattcggcg gaaaaaaagc
    37501 ttacgatgga tatcaaaaaa agtcagaagc agaggatgtg ctcgaaaaag caaaagagcg
    37561 ttacgaggag cataaaggaa aatttgagca ggctgaagct cagacatcat caaaattaac
    37621 aacgctcgga aataaagagc ttgagattgg taaagatttc agtgagttcg acaggattgc
    37681 tgctgagctc ctggcgcgtc tggaaaaaga gggcaataaa gacctgaaac tgagtgtccc
    37741 gcaacataac atcaataaga ttcataacct ggccatttca gcaacagaat atctcagtac
    37801 ggtcgtagct gccggagttt ccggggctgc tgcgggtttt gctgtgtata gcggagtgat
    37861 ggcatttgct gcggcttcca caggtacacc cattgcagcg ctttctggtg ccgcggctta
    37921 taacgcgacg atggcggcaa ttggaggggg ctctctcgcg gcagggggca tgggaatggc
    37981 cggcggcgca atggtattag gcaccgcagt ggctgcaccg ttgattgcca ttgcgggctg
    38041 ggcttacgac agacacgcga cgaaagctct tgaaaatgcc catcagtgtg cctgcaaagt
    38101 tgaacgggca gttaagaaaa tgaatctggc ccgggagcat tttgcaaaag ttaatcaata
    38161 cgttgatgaa attctcgctg cgctgaacag aatgcatgct gtatttatta aacactattt
    38221 tgagccgctt aaatccatgc attctgttat tacagaacaa caggatatca atgtatcaga
    38281 tgaaatcatt atgcttattg accatggata ttcactggcc gctattatga cggatattat
    38341 tacaacgcca ctgtttaaac cgcggaaaca ggaaaatggt gaggctgtga taaaagataa
    38401 cgttattgaa atggaaaagg atgataatgg aatgaatgtt attaataagg aagagctgga
    38461 tgatgtactg gccagttcgg ttgtgaaatt tgatgatttt tcatcccgga gttaatgatt
    38521 taccgggcgt taaagcccgg ctttacaaga agtgaaataa catgaatgtg gaaaaatttg
    38581 actgggcaga agagttacat cagaccatgg tgaagagcct ggttgcatca ttcgggctgg
    38641 attttctgtt gctaaatgat cgtaagggtg gtgatgttga cactatccat aatgtgcgta
    38701 atggcattta tgccacggag gctgagcgac agcggtatga aggtcgtggc gactatgatt
    38761 ctcatcatta ccacagccat gagaactaca tcgcgacgaa ccggaaagga aagcgtgcac
    38821 atcagtcagg gactcttact gatgcatata caggcgaagt gtttgcttca gacgataaaa
    38881 aaaatctgga tcatattatt tccgctaaag aaattcatga tgatccgggg agaattcttg
    38941 ctgaacggga tggtgcagaa ctggcaaatg acgcttcaaa tctggcattc actcatgagt
    39001 cgctcaataa atcaaagaaa gcagactcaa tggatgcgtt tatttgcacg ctgcagaaaa
    39061 acagagaaga tacgttaagg cagatagcag agcttgaatc tcaggcaaca ctgacagaaa
    39121 aagaaaagaa acgtctgata agtctgagaa aaaaagctga tgcagatact gagcgtatgg
    39181 aaagggctga taaatatgcc cgggaaaaat acgagaccac gattaatcag agctattacc
    39241 tgagtagtaa gtttgctact gctgtcgcca gtgcttcact taattccggt tttcgtatgg
    39301 ggacgcgaca gatgctgggg cttattcttg ccgagatctg gttcgaactg agggaacgaa
    39361 tcccgcatat tatcagacag caacggaact cattctgcgc cggcaaatgc ctgtccgaaa
    39421 taacgtcagc attacgtgcc tgccaggaac gagtacagga aaaattcagt tcttttttaa
    39481 ttgcctttaa agatggtgtc atcggaggga tactgtcagg tattaccaca acgctgttaa
    39541 atatattcct gacgacggaa agaatgattg tccggcttat cagggaaatg tggaacaacc
    39601 tcgttcaggc ctttaaagtc ctggtattta acccggaggg attatcgccg gggcagttag
    39661 ccaaagcggt cactaaactg atagcagcag gtgtggctgt tgccggtggg gtgatgataa
    39721 atgaggcgat ggcgcagatc cttatttttc catttggttc agagcttgct gccttttgtg
    39781 gtgcgctgac gacaggtatt ctcgggctgg tgatgaatta ttatctcgaa tatagcgagg
    39841 ttatgcagcg ggtatgggca tttctggaca aatttaagga caaattccag cggtcactgg
    39901 aatactttca ggcagtcaat gctgaacttg atcgctatct tgtggaactt actgcactgg
    39961 aattttctgt tgatgctgcc gcgttaaatc atttttcact tcatctggat agcgtaaaca
    40021 gtgagataga gcgcggactt ctgctgagag aagaagtcag ccgacgaggg attgcgttgc
    40081 cgtttgagtc aggaaatgtg gcttctgttc gtgactggtt aaataagcga tgaaattaca
    40141 ccctccattt ccctgtaacc agtgtggggc ctgttgtcgt catgtcaaca gggcgggtga
    40201 aacgcaatgt ctggatcgtg gtgacggtat ttgccgtcac tatcagactg attctcattt
    40261 gtgtgcaatt tatgataaac gcccacagat ttgccgggtt gaagaccagt atttactgaa
    40321 ttatcagtct cagtacagct ggcaggagtt tattgcatta aaccgggccg cctgccttat
    40381 tctgaataaa ctgtaatgcc attaatggcg gcgtcaggat agaaactgag agaacataaa
    40441 acagtaaaat gtcattccgt tcagcagcca gcgttgcttt ctccggctgc tgttattgta
    40501 aggcattttc gttaagaacg cagaaggaga ggatcgtgag attgctgtta aaagggctcg
    40561 tcggaatggc gctggtctgg cttgttattg gtttcgggat ggagttattt tttctcgccg
    40621 atcgggttca tccggcatta agagtaatcg ttggcctggg gatgttattc ctgatgataa
    40681 tgattgcggg cggtatgtgc cgttctcttc tgtcacgaaa gaaaacatca tgacgctcct
    40741 gtttacgccg ctacatttgt ggcggcttta tgattattca aaataatgaa ttcacctgtc
    40801 acggcatgtt cagcgaccag tgccggacag aaatatcgct tttacacaat tattatgaaa
    40861 attaatcttc cgttcataca ctggccgcgg atttgcaatg ataagtgcct cgtgttatct
    40921 gtgggggtct ttactctttc taatctcctg gtatcctcag gagcacatct gatctaccgg
    40981 gtgttattct ttatcctggc aattcttatt attaaggcga tgaaatcgct tacgctgcgt
    41041 tttattctgg cctttccgtt cacattactg accgcggcag atatcagtat cagcctgtat
    41101 tcatggtgta catttgacac cacattcaat gatggttttg caatcagtat cctacagaca
    41161 gatcctgatg aagcaataag aatgttcagg atgtatgttg tttatgttat cgcatttatt
    41221 atcctctttt tattgttctt ctgttcagca ataaataaaa cgtcctcatt gccatcggaa
    41281 aaggtcacgg taattacatt tttattgtta attacagtaa cgttatattc ttccttccag
    41341 tttgcgttga agaaacagta tcaaataaat gaagttgatc cttacatagt cgcttcccgg
    41401 tttgccacat ataccccttt ctttaatctg aactattttg cactggccgc aaaggagcat
    41461 cagagattaa tgacgattgc tgataccata cctcactacg atctggtgat tacggataac
    41521 aacactgacg tatttgttct ggtgattggt gaatctgcaa ggactgataa catgtccatt
    41581 tacgggtatt cgcgccctac cacaccagag cttcagaaac agaaatcacg actgaaactg
    41641 ttcactcagg caatcagtgg cgcaccttac accgcgctgg ctgttccact ggcgttatct
    41701 gccgatagtg tactgcatca tgatatccgt aattaccctg ataacattat caacatggcg
    41761 aatcaggcgg ggttcgatac ctggtggctc agtgcgcagt ccgctttccg acagaacgga
    41821 acggctgtgg ccagtatcgc catgcgggcc agaaacagaa tttatgtcag aggctatgat
    41881 gaattactgc tcccacacct ggctgaagca ctgaacagta atccggggag caggaaactg
    41941 attgttctgc atctgacggg cagccatgag ccagtctgca gtaactggcc cagagataaa
    42001 gccgtgttta aaccgctgga tacagaagaa gtctgctacg ataattccat tcattatacc
    42061 gacagtttgt tgggacaggt tttcaccatg ctggaaacgc gtcaggcatc cgtcatgtat
    42121 ttttcagatc acggtctgga gtatgacccg acaaaagaac atgcctattt tcatggtgga
    42181 ataaaaccca gtcagcaggc ttatcatgtt ccgatgttta tctggtacag cccgacactg
    42241 ggagagcatg tcgatcgcca gacggtgaac agcgtttttt ccactgcgta taatgattat
    42301 ctgattaatg catggatggg cgtaaccaga ccggctcaac cgaaaacgcc ggaggaggtg
    42361 attacccgct ggcaggggaa atccgtggtt tttgatgcca gtcataacgt gtttgactac
    42421 aaagtattac gaaagaactt taatgaactc caggaataat tcgttaaagt aatttacgac
    42481 tctattcttt tttaaaggaa actttcagaa aatgccccca gggcgataac ctggggaagg
    42541 gggactgtgt tattactggt ggataaatta ttacttgttt gaaagataca ctaatgcaat
    42601 atctattgat gctggatgct tgagtgtttc attcctgctg ctgaaaacgg caatatccag
    42661 ggccagacat attcaccgtt aatatttctg catttctgta caatttcctc agaaagtgtc
    42721 tcatcaattc tttcccgcaa cagcttctgc tgtcttaaaa tgctttcgca tatttcgaaa
    42781 tcatcttcct gcatcagcat ttcggcgcat ttaatgattt ctgctcgttc atgcagcaca
    42841 gaggaatggt tatagagcca gtttacaaac agcaatgctt ccggatattg tccccataat
    42901 ggtgagtatg ttgcatagta atcttctgcc gtat
//
LOCUS       DAJOSY010000025        41774 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJOSY010000025 DAJOSY010000000
VERSION     DAJOSY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.025"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            336..1904
                     /gene="cydA"
                     /locus_tag="OHN27_001506"
     CDS             336..1904
                     /gene="cydA"
                     /locus_tag="OHN27_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCQ2285973.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1920..3059
                     /gene="cydB"
                     /locus_tag="OHN27_001507"
     CDS             1920..3059
                     /gene="cydB"
                     /locus_tag="OHN27_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCQ2285974.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3074..3187
                     /gene="cydX"
                     /locus_tag="OHN27_001508"
     CDS             3074..3187
                     /gene="cydX"
                     /locus_tag="OHN27_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCQ2285975.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3187..3480
                     /gene="ybgE"
                     /locus_tag="OHN27_001509"
     CDS             3187..3480
                     /gene="ybgE"
                     /locus_tag="OHN27_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCQ2285976.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3630..4034
                     /gene="ybgC"
                     /locus_tag="OHN27_001510"
     CDS             3630..4034
                     /gene="ybgC"
                     /locus_tag="OHN27_001510"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCQ2285977.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4031..4723
                     /gene="tolQ"
                     /locus_tag="OHN27_001511"
     CDS             4031..4723
                     /gene="tolQ"
                     /locus_tag="OHN27_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCQ2285978.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4727..5155
                     /gene="tolR"
                     /locus_tag="OHN27_001512"
     CDS             4727..5155
                     /gene="tolR"
                     /locus_tag="OHN27_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCQ2285979.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5220..6521
                     /gene="tolA"
                     /locus_tag="OHN27_001513"
     CDS             5220..6521
                     /gene="tolA"
                     /locus_tag="OHN27_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCQ2285980.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPA
                     GSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKP
                     EGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6654..7946
                     /gene="tolB"
                     /locus_tag="OHN27_001514"
     CDS             6654..7946
                     /gene="tolB"
                     /locus_tag="OHN27_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCQ2285981.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7981..8502
                     /gene="pal"
                     /locus_tag="OHN27_001515"
     CDS             7981..8502
                     /gene="pal"
                     /locus_tag="OHN27_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCQ2285982.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8512..9303
                     /gene="cpoB"
                     /locus_tag="OHN27_001516"
     CDS             8512..9303
                     /gene="cpoB"
                     /locus_tag="OHN27_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCQ2285983.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9468..9543
                     /locus_tag="OHN27_001517"
     tRNA            9468..9543
                     /locus_tag="OHN27_001517"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9501..9503,aa:Lys,seq:ttt)
     gene            9679..9754
                     /locus_tag="OHN27_001518"
     tRNA            9679..9754
                     /locus_tag="OHN27_001518"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9712..9714,aa:Val,seq:tac)
     gene            9757..9832
                     /locus_tag="OHN27_001519"
     tRNA            9757..9832
                     /locus_tag="OHN27_001519"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9790..9792,aa:Lys,seq:ttt)
     gene            9884..9959
                     /locus_tag="OHN27_001520"
     tRNA            9884..9959
                     /locus_tag="OHN27_001520"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9917..9919,aa:Val,seq:tac)
     gene            9963..10038
                     /locus_tag="OHN27_001521"
     tRNA            9963..10038
                     /locus_tag="OHN27_001521"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9996..9998,aa:Lys,seq:ttt)
     gene            10185..10260
                     /locus_tag="OHN27_001522"
     tRNA            10185..10260
                     /locus_tag="OHN27_001522"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10218..10220,aa:Lys,seq:ttt)
     gene            10393..10468
                     /locus_tag="OHN27_001523"
     tRNA            10393..10468
                     /locus_tag="OHN27_001523"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10426..10428,aa:Lys,seq:ttt)
     gene            10746..11789
                     /gene="nadA"
                     /locus_tag="OHN27_001524"
     CDS             10746..11789
                     /gene="nadA"
                     /locus_tag="OHN27_001524"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCQ2285984.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKECNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11827..12546
                     /gene="pnuC"
                     /locus_tag="OHN27_001525"
     CDS             11827..12546
                     /gene="pnuC"
                     /locus_tag="OHN27_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCQ2285985.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12543..13484)
                     /gene="zitB"
                     /locus_tag="OHN27_001526"
     CDS             complement(12543..13484)
                     /gene="zitB"
                     /locus_tag="OHN27_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCQ2285986.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGSFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13598..13978)
                     /locus_tag="OHN27_001527"
     CDS             complement(13598..13978)
                     /locus_tag="OHN27_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCQ2285987.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14294..15346
                     /gene="aroG"
                     /locus_tag="OHN27_001528"
     CDS             14294..15346
                     /gene="aroG"
                     /locus_tag="OHN27_001528"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCQ2285988.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15504..16256)
                     /gene="gpmA"
                     /locus_tag="OHN27_001529"
     CDS             complement(15504..16256)
                     /gene="gpmA"
                     /locus_tag="OHN27_001529"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCQ2285989.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16458..17498)
                     /gene="galM"
                     /locus_tag="OHN27_001530"
     CDS             complement(16458..17498)
                     /gene="galM"
                     /locus_tag="OHN27_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCQ2285990.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17492..18640)
                     /gene="galK"
                     /locus_tag="OHN27_001531"
     CDS             complement(17492..18640)
                     /gene="galK"
                     /locus_tag="OHN27_001531"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCQ2285991.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18644..19690)
                     /gene="galT"
                     /locus_tag="OHN27_001532"
     CDS             complement(18644..19690)
                     /gene="galT"
                     /locus_tag="OHN27_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCQ2285992.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19700..20716)
                     /gene="galE"
                     /locus_tag="OHN27_001533"
     CDS             complement(19700..20716)
                     /gene="galE"
                     /locus_tag="OHN27_001533"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCQ2285993.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20977..22449)
                     /gene="modF"
                     /locus_tag="OHN27_001534"
     CDS             complement(20977..22449)
                     /gene="modF"
                     /locus_tag="OHN27_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCQ2285994.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPSEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(22517..23305)
                     /gene="modE"
                     /locus_tag="OHN27_001535"
     CDS             complement(22517..23305)
                     /gene="modE"
                     /locus_tag="OHN27_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCQ2285995.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23434..23583
                     /gene="acrZ"
                     /locus_tag="OHN27_001536"
     CDS             23434..23583
                     /gene="acrZ"
                     /locus_tag="OHN27_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCQ2285996.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23750..24523
                     /gene="modA"
                     /locus_tag="OHN27_001537"
     CDS             23750..24523
                     /gene="modA"
                     /locus_tag="OHN27_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2285997.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQFKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASVQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTIK"
     gene            24523..25212
                     /gene="modB"
                     /locus_tag="OHN27_001538"
     CDS             24523..25212
                     /gene="modB"
                     /locus_tag="OHN27_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCQ2285998.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25215..26273
                     /gene="modC"
                     /locus_tag="OHN27_001539"
     CDS             25215..26273
                     /gene="modC"
                     /locus_tag="OHN27_001539"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCQ2285999.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26274..27092)
                     /gene="ybhA"
                     /locus_tag="OHN27_001540"
     CDS             complement(26274..27092)
                     /gene="ybhA"
                     /locus_tag="OHN27_001540"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCQ2286000.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVIKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27247..28242
                     /gene="pgl"
                     /locus_tag="OHN27_001541"
     CDS             27247..28242
                     /gene="pgl"
                     /locus_tag="OHN27_001541"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCQ2286001.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28283..29236)
                     /locus_tag="OHN27_001542"
     CDS             complement(28283..29236)
                     /locus_tag="OHN27_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2286002.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEAQELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29420..30472
                     /locus_tag="OHN27_001543"
     CDS             29420..30472
                     /locus_tag="OHN27_001543"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCQ2286003.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30548..31981
                     /locus_tag="OHN27_001544"
     CDS             30548..31981
                     /locus_tag="OHN27_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCQ2286004.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            32164..34425
                     /locus_tag="OHN27_001545"
     CDS             32164..34425
                     /locus_tag="OHN27_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCQ2286005.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVSRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34659..35942)
                     /locus_tag="OHN27_001546"
     CDS             complement(34659..35942)
                     /locus_tag="OHN27_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCQ2286006.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(36094..36570)
                     /locus_tag="OHN27_001547"
     CDS             complement(36094..36570)
                     /locus_tag="OHN27_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCQ2286007.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(36629..37918)
                     /gene="bioA"
                     /locus_tag="OHN27_001548"
     CDS             complement(36629..37918)
                     /gene="bioA"
                     /locus_tag="OHN27_001548"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCQ2286008.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            38005..39045
                     /gene="bioB"
                     /locus_tag="OHN27_001549"
     CDS             38005..39045
                     /gene="bioB"
                     /locus_tag="OHN27_001549"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCQ2286009.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            39042..40196
                     /gene="bioF"
                     /locus_tag="OHN27_001550"
     CDS             39042..40196
                     /gene="bioF"
                     /locus_tag="OHN27_001550"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCQ2286010.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            40183..40938
                     /gene="bioC"
                     /locus_tag="OHN27_001551"
     CDS             40183..40938
                     /gene="bioC"
                     /locus_tag="OHN27_001551"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCQ2286011.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPKGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            40931..41608
                     /gene="bioD"
                     /locus_tag="OHN27_001552"
     CDS             40931..41608
                     /gene="bioD"
                     /locus_tag="OHN27_001552"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCQ2286012.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         9999 a        10363 c        11155 g        10257 t
ORIGIN      
        1 atgtataagt cttggaaatg ggcatcaaaa agagataaat tgttctcgat caaattggct
       61 gaaaggcggt aatttagcta taaattgatc accgtcgaaa aatgcaaatt tgcttcaaca
      121 aaaacctgtt tattgtaagg attttgcggc gtaatatata cgtgggatca atttgagttt
      181 ttattaacat gtttgcaacc tttctttacg ccgtttttgt gtgcattcac atggtatgat
      241 gaaagtgttc aaacaaattt ctattggggc atgcgtgtga ccctttctaa cggggttcac
      301 tctcggagtc ttcatgcgat gagcaaggag tcatgatgtt agatatagtc gaactgtcgc
      361 gcttacagtt tgccttgacc gcgatgtacc acttcctttt tgtgccactg acgctcggta
      421 tggcgttcct gctggccatt atggaaacgg tctacgtcct ctccggcaaa cagatttata
      481 aagatatgac caagttctgg ggcaagttgt ttggtatcaa cttcgctctg ggtgtggcta
      541 ccggtctgac catggagttc cagttcggga ctaactggtc ttactattcc cactatgtag
      601 gggatatctt cggtgcgccg ctggcaatcg aaggtctgat ggccttcttc ctcgaatcca
      661 cctttgtagg tctgttcttc ttcggttggg atcgtctggg taaagttcag catatgtgtg
      721 tcacctggct ggtggcgctc ggttcaaacc tgtccgcact gtggattctg gttgcgaacg
      781 gctggatgca aaacccaatc gcgtccgatt tcaactttga aactatgcgt atggagatgg
      841 tgagcttctc cgagctggtg cttaacccgg ttgctcaggt gaaattcgtt cacactgtag
      901 cgtctggtta tgtgactggc gcgatgttca tcctcggtat cagcgcatgg tatatgctga
      961 aaggtcgtga cttcgccttc gctaaacgct cctttgctat cgctgccagc ttcggtatgg
     1021 ctgctgttct gtctgttatt gttctgggtg atgaatccgg ctacgaaatg ggcgacgtgc
     1081 agaaaaccaa actggctgct attgaagccg agtgggaaac gcaacctgcg cctgctgcct
     1141 ttactctgtt cggcattcct gatcaggaag aggagacgaa caaatttgcg attcagatcc
     1201 cttacgcact gggcatcatt gcaacgcgtt ccgtggatac cccggttatc ggcctgaaag
     1261 agctgatggt gcagcatgaa gaacgcattc gtaacgggat gaaggcgtac tctctgctcg
     1321 aacaactgcg ttctggttct accgaccagg cggttcgtga ccagttcaat agcatgaaga
     1381 aagacctcgg ttacggtctg ctgctgaaac gctatacgcc aaacgtggct gatgcgactg
     1441 aagcgcagat tcaacaggca accaaagact ccatcccgcg tgtagcgccg ctgtactttg
     1501 cgttccgtat catggtggcg tgtggcttcc tgcttctggc aatcatcgcg ctctctttct
     1561 ggagtgtcat ccgcaaccgc attggcgaga aaaaatggct tctgcgcgcc gcgctgtacg
     1621 gtattccgct gccgtggatt gctgtagaag cgggctggtt cgtggctgaa tatggccgcc
     1681 aaccgtgggc tatcggtgaa gtgctgccga cagctgtggc gaactcgtca ctgaccgcag
     1741 gcgatctcat cttctcaatg gtgctgattt gcggcctgta taccctgttc ctggtggcag
     1801 aattgttctt aatgttcaag tttgcacgcc tcggcccaag cagcctgaaa accggtcgct
     1861 atcactttga gcagtcttcc acgactactc agccggcacg ctaagacagg agtcgtcaaa
     1921 tgatcgatta tgaagtattg cgttttatct ggtggctgct ggttggcgtt ctgctgattg
     1981 gttttgcagt cactgacggt ttcgacatgg gggtgggcat gctcacccgt ttcctcggtc
     2041 gtaacgacac cgagcgtcga attatgatta actccattgc accacactgg gacggtaacc
     2101 aggtttggct gatcaccgcg ggcggcgcac tctttgctgc ctggccgatg gtctatgccg
     2161 ctgcgttctc cggcttctat gtggcgatga tcctcgtgct ggcgtctttg ttcttccgtc
     2221 cggtcggttt tgactaccgc tccaagattg aagaaacccg ctggcgtaac atgtgggact
     2281 ggggcatctt cattggtagc ttcgttccgc cgctggtaat tggtgtagcg ttcggtaacc
     2341 tgttgcaggg cgtaccgttc aacgttgatg aatatctgcg tctgtactac accggtaact
     2401 tcttccagtt gcttaacccg ttcggcctgc tggcaggcgt ggtgagcgta gggatgatca
     2461 ttactcaggg cgcaacctat ctgcaaatgc gtaccgtggg cgaactgcac ctgcgtaccc
     2521 gtgcaacggc tcaggtggct gcgctggtga cactggtctg tttcgcactg gctggcgtat
     2581 gggtgatgta cggtatcgat ggttatgtcg tgaaatcgac aatggaccat tacgcagcct
     2641 ctaacccact gaataaagaa gtggttcgtg aagctggcgc atggctggtt aacttcaaca
     2701 acacgccaat tctgtgggct attccggcac tgggtgtggt tctgccgctg ctgaccatcc
     2761 tgactgcacg tatggataaa gccgcgtggg cgtttgtgtt ctcctccctg acgctggcct
     2821 gcatcatcct gacagccggt atcgcaatgt tcccgtttgt gatgccgtcc agcaccatga
     2881 tgaacgcaag tctgacaatg tgggatgcaa cttccagcca gctgacgctt aacgtcatga
     2941 cctgggttgc ggtggttctg gtaccgatca ttctgctcta caccgcctgg tgttactgga
     3001 aaatgttcgg tcgtatcacc aaagaagata ttgaacgtaa cacccactct ctgtactaag
     3061 taaggagcta aaaatgtggt atttcgcatg gattctggga acgcttcttg cctgttcgtt
     3121 tggggtaatc accgcgctgg cgcttgaaca cgtcgaatca ggcaaagccg gtcaagaaga
     3181 catctgatga gtaagattat cgcgactttg tatgcggtaa tggacaagcg ccccctgcgg
     3241 gcgctttcct tcgtgatggc gcttctgtta gcaggatgta tgttttggga cccatctcgt
     3301 tttgccgcga agaccagtga actggaaatc tggcatggtt tattgctgat gtgggccgtc
     3361 tgtgctggtg tgattcacgg cgtgggcttt cgtccgcaga aggttctttg gcaagggatt
     3421 ttttgcccat tgcttgccga tattgttctc attgtcgggc tgattttctt cttcttttaa
     3481 atcagaattc tctttaaaaa attatgggcc gctccaggcc cataaatttt tacgctccct
     3541 taacttgccc tcattcccaa acctcaatcg cgcgcgtata gtagcagcgt ttaaaagctc
     3601 taacttttgt tgcattaccg ggatgtaaag tgaatacaac gctgtttcga tggccggttc
     3661 gcgtctacta tgaagatacc gatgccggtg gtgtggtgta ccacgccagt tacgtcgctt
     3721 tttatgaaag agcacgcaca gagatgctgc gtcatcatca cttcagtcag caggcgctga
     3781 tggctgaacg cgttgccttt gtggtacgta aaatgacggt ggaatattac gcacctgcgc
     3841 ggctcgacga tatgctcgaa atacagactg aaataacatc aatgcgtggc acctctttgg
     3901 ttttcacgca acgtattgtc aacgccgaga atactttgct gaatgaagca gaggttctgg
     3961 ttgtttgcgt tgacccactc aaaatgaagc ctcgtgcgct tcccaagtct attgtcgcgg
     4021 agtttaagca gtgactgaca tgaatatcct tgatttgttc ctgaaggcta gccttctggt
     4081 taaacttatc atgttgattt tgattggttt ttcaatcgca tcttgggcca ttattatcca
     4141 gcggacccgt attcttaacg cagcggcgcg cgaagccgaa gcgtttgaag ataaattctg
     4201 gtctggaatc gaactctctc gcctctatca agagagccag gggaaacggg ataatctgac
     4261 tggttcggaa caaatctttt acagcgggtt caaagagttt gtgcgcctgc atcgtgccaa
     4321 tagccatgcg ccggaagccg tagtggaagg ggcgtcgcgt gctatgcgta tctccatgaa
     4381 ccgtgaactt gaaaatctgg aaacgcacat tccgttcctc ggtacggttg gctccatcag
     4441 cccgtatatt ggtctgtttg gtacggtctg ggggatcatg cacgccttta tcgccctcgg
     4501 ggcggtaaaa caagcaacac tgcaaatggt tgcgcccggt atcgcagaag cgttgattgc
     4561 gactgcaatt ggtctgtttg ccgctatccc ggcagttatg gcctacaacc gcctcaacca
     4621 gcgcgtaaac aaactggaac tgaattacga caactttatg gaagagttta ccgcgattct
     4681 gcaccgccag gcgtttaccg ttagcgagag caacaagggg taagccatgg ccagagcgcg
     4741 tggacgaggt cgtcgcgatc tcaagtccga aatcaacatt gtaccgttgc tggacgtact
     4801 gctggtgctg ttgctgatct ttatggcgac agcgcccatc atcacccaga gcgtggaggt
     4861 cgatctgcca gacgctactg aatcacaggc ggtgagcagt aacgataatc cgccagtgat
     4921 tgttgaagtg tctggtattg gtcagtacac cgtggtggtt gagaaagatc gcctggagcg
     4981 tttaccacca gagcaggtgg tggcggaagt gtccagccgt ttcaaggcca acccgaaaac
     5041 ggtctttctg atcggtggcg caaaagatgt gccttacgat gaaataatta aagcactgaa
     5101 cttgttacat agtgcgggtg tgaaatcggt tggtttaatg acgcagccta tctaaacatc
     5161 tgcgtttccc ttgcttgaaa gagagcgggt aacaggcgaa cagtttttgg aaaccgagag
     5221 tgtcaaaggc aaccgaacaa aacgacaagc tcaagcgggc gataattatt tcagcagtgc
     5281 tgcatgtcat cttatttgcg gcgctgatct ggagttcgtt cgatgagaat atagaagctt
     5341 cagccggagg cggcggtggt tcgtccatcg acgctgtcat ggttgattca ggtgcggtag
     5401 ttgagcagta caaacgcatg caaagccagg aatcaagcgc gaagcgttct gatgaacagc
     5461 gcaagatgaa ggaacagcag gctgctgaag aactgcgtga gaaacaagcg gctgaacagg
     5521 aacgcctgaa gcaacttgag aaagagcggt tagcggctca ggagcagaaa aagcaggctg
     5581 aagaagccgc aaaacaggcc gagttaaagc agaagcaagc tgaagaggcg gcagcgaaag
     5641 cggcggcaga tgctaaagcg aaggccgaag cagatgctaa agctgcggaa gaagcagcga
     5701 agaaagcggc tgcagacgca aagaaaaaag cagaagcaga agccgccaaa gccgcagccg
     5761 aagcgcagaa aaaagccgag gcagccgctg cggcactgaa gaagaaagcg gaagcggcag
     5821 aagcagctgc agctgaagca agaaagaaag cggcaactga agctgctgaa aaagccaaag
     5881 cagaagctga gaagaaagcg gctgctgaaa aggctgcagc tgataagaaa gcggcagcag
     5941 agaaagctgc agccgacaaa aaagcagcag aaaaagcggc tgctgaaaag gcagcagctg
     6001 ataagaaagc agcggcagaa aaagccgccg cagacaaaaa agcggcagcg gcagaaaaag
     6061 ccgccgcaga caaaaaagcg gcagcggcaa aagctgcagc tgaaaaagcc gctgcagcaa
     6121 aagcggccgc agaggcagat gatattttcg gtgagctaag ctctggtaag aatgcaccga
     6181 aaacgggggg aggggcgaaa gggaacaatg cttcgcctgc cgggagtggt aatactaaaa
     6241 acaatggcgc atcaggggcc gatatcaata actatgccgg gcagattaaa tctgctatcg
     6301 aaagtaagtt ctatgacgca tcgtcctatg caggcaaaac ctgtacgctg cgcataaaac
     6361 tggcacccga tggtatgtta ctggatatca aacctgaagg tggcgatccc gcactttgtc
     6421 aggctgcgtt ggcagcagct aaacttgcga agatcccgaa accaccaagc caggcagtat
     6481 atgaagtgtt caaaaacgcg ccattggact tcaaaccgta atcgcgatgt tgactgttcg
     6541 gacggtcaac atcaggcacc ggttgccacg gggttctggt agttttgtgt attttagttt
     6601 gttaacattc tgctaaatta tcgtgggcca tcggtccaga taagggagat atgatgaagc
     6661 aggcattacg agtagcattt ggttttctca tactgtgggc atcagttctg catgctgaag
     6721 tccgcattgt gatcgacagc ggtgtagatt ccggtcgtcc tattggtgtt gttcctttcc
     6781 agtgggcggg gcctggtgcg gcacctgaag atattggcgg catcgttgct gctgacttgc
     6841 gtaacagcgg taaatttaat ccgttagatc gcgctcgtct gccacagcag ccgggtagtg
     6901 cgcaggaagt acaaccagct gcatggtccg cactgggcat tgacgctgta gttgtcggtc
     6961 aggtcactcc gaatccggat ggttcttaca atgttgctta tcaacttgtt gacactggcg
     7021 gcgcaccggg tactgtactt gctcagaact cgtacaaagt gaacaagcag tggctgcgtt
     7081 atgctggtca taccgccagt gatgaagtgt ttgaaaaact gaccggcatt aaaggtgcgt
     7141 tccgtactcg tattgcctac gttgttcaga ccaacggcgg tcagttcccg tatgaactgc
     7201 gcgtatctga ctatgacggt tacaaccagt ttgtcgttca ccgttcaccg cagccgctga
     7261 tgtctccggc gtggtcacca gacggttcta aactggctta tgtgaccttc gaaagcggtc
     7321 gttccgcgct ggttattcag acgctggcaa atggcgctgt acgtcaggtg gcttcattcc
     7381 cgcgtcacaa cggtgcacct gcattctcgc cagacggcag caaactggca ttcgccttgt
     7441 cgaaaaccgg tagtctgaac ctgtacgtaa tggatttggc ttctggtcag atccgccagg
     7501 tgactgatgg tcgcagtaac aataccgaac cgacctggtt cccggatagc cagaacctgg
     7561 catttacttc tgaccaggcc ggtcgtccgc aggtttataa agtgaatatc aacggcggtg
     7621 cgccacaacg tattacctgg gaaggttcgc agaaccagga tgcggatgtc agcagcgacg
     7681 gtaaatttat ggtaatggtc agctccaatg gtgggcagca gcacattgcc aaacaagatc
     7741 tggcaacggg aggcgtacaa gttctgtcgt ccacgttcct ggatgaaacg ccaagtctgg
     7801 cacctaacgg cactatggta atctacagct cttctcaggg gatgggatcc gtgctgaatt
     7861 tggtttctac agatgggcgt ttcaaagcgc gtcttccggc aactgatgga caggtcaaat
     7921 tccctgcctg gtcgccgtat ctgtgataat aattaattga atagtaaagg aatcattgaa
     7981 atgcaactga acaaagtgct gaaagggctg atgattgctc tgcctgttat ggcaattgcg
     8041 gcatgttctt ccaacaagaa cgccagcaat gacggcagcg aaggcatgct gggtgccggc
     8101 actggtatgg atgcgaacgg cggcaacggc aacatgtctt ccgaagagca ggctcgtctg
     8161 caaatgcaac agctgcagca gaacaacatc gtttacttcg atctggacaa gtacgatatc
     8221 cgttctgact tcgctcaaat gctggatgca catgcaaact tcctgcgtag caacccgtct
     8281 tacaaagtca ccgtagaagg tcacgcggac gaacgtggta ctccggaata caacatctcc
     8341 ctgggtgaac gtcgtgcgaa cgccgttaag atgtacctgc agggtaaagg cgtttctgca
     8401 gaccagatct ccatcgtttc ttacggtaaa gaaaaacctg cagtactggg tcatgacgaa
     8461 gcggcatact ccaaaaaccg tcgtgcggta ctggtttact aagagaattg catgagcagt
     8521 aacttcagac atcaactatt gagtctgtcg ttactggttg gtatagcggc cccctgggcc
     8581 gcttttgctc aggcaccaat cagtagtgtc ggctcaggct cggtcgaaga ccgcgtcact
     8641 caacttgagc gtatttctaa tgctcacagc cagcttttaa cccaactcca gcaacaactt
     8701 tctgataatc aatccgatat tgattccctg cgtggtcaga ttcaggaaaa tcagtatcaa
     8761 ctgaatcagg tcgtggagcg gcaaaagcag atcctgttgc agatcgacag cctcagcagc
     8821 ggtggtgcag cggcgcaatc aaccagcagc gatcaaagcg gtgcggcggc atcaacgacg
     8881 ccgacagctg atgctggtac tgcgaatgct ggcgcgccgg tgaaaagcgg cgatgcaaac
     8941 acggattaca atgcagctat tgcgctggtg caggataaat cccgccagga tgacgcaatg
     9001 gtggcatttc agaatttcat caaaaattac cctgattcaa cttacctgcc aaacgccaat
     9061 tattggctgg gtcagttaaa ctacaacaag ggtaaaaaag atgatgcggc gtactatttt
     9121 gcttcggtag tgaaaaacta tccgaagtca ccaaaggctg ctgatgcgat gtttaaagtc
     9181 ggcgtcatca tgcaggacaa aggtgacacc gcaaaagcga aagccgtata ccagcaggtt
     9241 atcagtaaat accctggtac tgatggcgct aaacaggcac aaaaacgtct gaacgcgatg
     9301 taatgcataa cacacgacca gaagtcgcat tatttctggt cgtgtcgtgc gaatcataag
     9361 cagttgagtg atctgcatcg aaatttttgt tgcgctcaag tctgaaatca gtaatatatg
     9421 ccgccgttgc cacgggatat caaacaaacc gaaagcaacg aaaaagtggg tcgttagctc
     9481 agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca
     9541 ccaatcgcta aggtggaagc ggtagtaaaa cgtgaaggat aacgttgcat cagcaacggc
     9601 ccgaagggcg agacgaagtc gagtcatcct gcacgaccca ccactaacat agttagttgt
     9661 agtatccagc gtagtattgg gtgattagct cagctgggag agcacctccc ttacaaggag
     9721 ggggtcggcg gttcgatccc gtcatcaccc accaccgggt cgttagctca gttggtagag
     9781 cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg cacgacccac cactaattac
     9841 ggtgggttcg gtggaagtag tttgtagtat ccagcgcagt atcgggtgat tagctcagct
     9901 gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac
     9961 tcgggtcgtt agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga
    10021 atcctgcacg acccaccagt tttaacatca aactcagatg ttaagcgtga aggataacgt
    10081 tacgccagca acggcccgta gggcgagcga agcgagtcat cctgcacgag ccaccaattt
    10141 taaagattgg ttccgagtag aaatctttca ggtaacaccc atatgggtcg ttagctcagt
    10201 tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca cgacccacca
    10261 atttaaaggt ggttactggt agagaacgtg aaggataacg ttgcgttagc aacggcccga
    10321 agggcgagac gaagtccagt catcctgcac gacccaccat cctgaatgat taaagcagca
    10381 taatcccgca aggggtcgtt agctcagttg gtagagcagt tgacttttaa tcaattggtc
    10441 gcaggttcga atcctgcacg acccaccaat gtaaaaaagc gccctaaagg cgcttttttg
    10501 ctattcaggc atctctcaat ttcaattctt gaccctgatg acatactccg cgcttgctgc
    10561 gtaaacaact ttacgtcgga ggaataagac gcaaagtgtg gcattcggca tctcatacgt
    10621 aattcgagtg acttttcccc accattcgac tatcttgttt agcatataaa acaaattaca
    10681 ccgataacag cgaatattac gctaatgtcg gttttaacgt taagcctgta aaacgagatg
    10741 gtaagatgag cgtaatgttt gatccagaca cggcgattta tcctttcccc ccgaagccga
    10801 cgccgttaag cattgatgaa aaagcgtatt accgcgagaa gataaaacgt ctgctaaaag
    10861 aatgtaatgc ggtgatggtt gcccactact ataccgatcc cgaaattcaa caactggcag
    10921 aagaaaccgg tggctgtatt tctgattctc tggaaatggc gcgcttcggt gcaaagcatc
    10981 ccgcttctac tttgttagtc gctggggtga gatttatggg agaaaccgcc aaaattctca
    11041 gtccggaaaa aacaattctg atgccgacac ttcaggctga atgttcactg gatctcggct
    11101 gccctgttga agaatttaac gcattttgcg atgcccatcc cgatcgtact gtcgtcgtct
    11161 acgccaacac ttctgctgcg gtaaaagcgc gcgcagattg ggtggtaact tcaagcattg
    11221 ccgtcgaact tattgatcat cttgatagtt tgggtgaaaa aatcatctgg gcacccgaca
    11281 aacatctggg gcgttacgtg caaaaacaga cgggtggaga cattctatgc tggcagggtg
    11341 cctgtattgt gcatgatgaa tttaagactc aggcgttaac ccgcttgcaa gaagaatacc
    11401 cggatgctgc catactggtg catccagaat caccacaagc tattgtcgat atggcggatg
    11461 cggtcggttc caccagtcaa ctgatcgctg ctgcgaaaac attgccacat cagaggctta
    11521 ttgtggcaac cgatcggggt attttctaca aaatgcagca ggcggtgcca gataaagagt
    11581 tactggaagc accaaccgca ggtgagggtg caacctgccg cagctgcgcg cattgtccgt
    11641 ggatggccat gaatggcctt caggccatcg cagaggcatt agaacaggaa ggaagcaatc
    11701 acgaggttca tgttgatgaa aggctgcgag agagggcgct ggtgccgctc aatcgtatgc
    11761 tggattttgc ggctacacta cgtggataac gaataataag gcgtaacgtt acgctttggg
    11821 ggaaagatgg atttttttag tgtgcagaat atcctggtac atataccaat aggggcaggc
    11881 ggttatgatc tctcatggat cgaagcggta ggcacgatcg ccgggttgct gtgtattggc
    11941 cttgccagtc tggagaagat cagcaactac ttctttggcc tgatcaacgt caccttgttt
    12001 ggcattattt tctttcagat tcagctgtat gccagcctgc tattacaggt gtttttcttt
    12061 gccgcgaata tttacggttg gtatgcgtgg tcgcgacaaa ccagtcagaa cgaggcggag
    12121 ttgaaaattc gctggttgcc attgccgaag gcactcagct ggttggcggt ttgcgttgtt
    12181 tcgattggtc tgatgacggt atttatcaat ccggtgtttg catttttgac ccgcgtggca
    12241 gtcatgatca tgcaagcatt aggattacag gttgtgatgc ctgaactgca accggacgct
    12301 ttcccgttct gggattcatg catgatggtg ttatctatcg tggcaatgat tctgatgacg
    12361 cgtaagtatg tggaaaactg gctgttgtgg gtgattatta acgtgattag cgtcgttatt
    12421 tttgcacttc agggcgttta cgccatgtct ctggagtaca tcatcctgac ctttattgcg
    12481 ctcaacggca gccggatgtg gatcaacagc gcacgtgaaa gaggctcacg cgcgctgtcc
    12541 cattaatggt gatgatgtga atggccagac accccttcat tcaggtggca gtctggccca
    12601 tgacaaggtt gatattccat ctgaatggtg gcgtgctcaa tctgatagtg atccatcagg
    12661 tagtgttgga tctgatccaa caaggcatcg tgatcgtgtg gcgggatcac ctgcacatgc
    12721 aacgtcatca ccggcttctc gcctaccatc catacatgta catggtgcac attgcggact
    12781 tccgggattt cccggcacat acgacgcttc agttcagcga tatccagcga taccggtgca
    12841 ccttcaagta attcattcac gctatctttc aacagtcgcc acgcgctacg cagaaccaga
    12901 agcgacacca gtatcgagag aatggggtca gcaggtgtcc agccagtcca gataataatc
    12961 aacgcggcaa taatagcccc aaccgaaccc agcaggtccc cgagcacatg cagtgccgct
    13021 gctcgtacgt tgaggttttt ctcttcgctg ccgtgatgaa gtaaccagaa agaaagtata
    13081 tttgccagca acccagccac ggcaattgcc atcatcatgc cgccctcgac cggacgcggc
    13141 gtgcggaagc gttctatcgc ctcccagaca atcaaaatgg taattaccac gagggctatg
    13201 gcattcacaa acgccgcgag cgtggtcagt cttagccaac cgaaagtgtg gcgaatggtg
    13261 ggaggacgac gtgaaaattg cacggcgagt agggcgaaaa gcagggcggc agtatcggtc
    13321 aacatatgac ccgcatcggc cagcaatgcc agagaaccag aaagaaaact accaacgact
    13381 tctaccagca taaacccggc agtcacgccg aaagcataca acaagcgacg agcattatta
    13441 tcttctggca ggtgtgagga cgtgtgtgag tgtgagtgcg ccatgacgag tcatccctta
    13501 attattctca cttaacttta tgacatcata ctgcttttag aagtgaaaaa ttaaaaggga
    13561 gagactccgc tctcccatta ttggctattt tgcagggtta ctgcgtggta ccgtcggttt
    13621 tggtatcgac atcattattg atgccatcac cggtttgtac ctttttattg atatccggac
    13681 agcgaccatc tttgcacatg gtgttcttgt gctcttcatc tttggtcatt ccgtcattgt
    13741 tcattgaaga accatccgaa tgcagcattg tgccgccaga accggtattt accccgttat
    13801 tgtcgacgtt atttggcgcg acattttcac gggcgtcagg ggctacctgg cccgcatcag
    13861 ctgcggcgtt tgcctggccg ttattagttt gcgctccgct atcggcagcc agtgcggcac
    13921 cgctggcaag gcttagagtg gcagtcagaa ataatgtggc cagttttgtc attttcatag
    13981 gatgctcctg ttatggtcgt tatgtcggat aacctcttcc aacagtgcat ttgcaggtga
    14041 atataaggca ttggtttaag atttcagcca ggttatgaaa cgcagcagag aatcttgaaa
    14101 taattaacaa acaaaggagt tacagttaga aattgtagga gagatctcgt ttttcgcgac
    14161 aatctggcgt ttttcttgct aattccagga ttaatccgtt catagtgtaa aaccccgttt
    14221 acacattctg acggaagata tagattggaa gtattgcatt cactaagata agtatggcaa
    14281 cactggaaca gacatgaatt atcagaacga cgatttacgc atcaaagaaa tcaaagagtt
    14341 acttcctcct gtcgcattgc tggaaaaatt ccccgctact gaaaatgccg cgaatacggt
    14401 tgcccatgcc cgaaaagcga tccataagat cctgaaaggt aatgatgatc gcctgttggt
    14461 tgtgattggc ccatgctcaa ttcatgatcc tgtcgcggca aaagagtatg ccactcgctt
    14521 gctggcgctg cgtgaagagc tgaaagatga gctggaaatc gtaatgcgcg tctattttga
    14581 aaagccgcgt accacggtgg gctggaaagg gctgattaac gatccgcata tggataatag
    14641 cttccagatc aacgacggtc tgcgtatagc ccgtaaattg ctgcttgata ttaacgacag
    14701 cggtctgcca gcggcaggtg agtttctcga tatgatcacc ccacaatatc tcgctgacct
    14761 gatgagctgg ggcgcaattg gcgcacgtac caccgaatcg caggtgcacc gcgaactggc
    14821 atcagggctt tcttgtccgg tcggcttcaa aaatggcacc gacggtacga ttaaagtggc
    14881 tatcgatgcc attaatgccg ccggtgcgcc gcactgcttc ctgtccgtaa cgaaatgggg
    14941 gcattcggcg attgtgaata ccagcggtaa cggcgattgc catatcattc tgcgcggcgg
    15001 taaagagcct aactacagcg cgaagcacgt tgctgaagtg aaagaagggc tgaacaaagc
    15061 aggcctgcca gcacaggtga tgatcgattt cagccatgct aactcgtcca aacaattcaa
    15121 aaagcagatg gatgtttgtg ctgacgtttg ccagcagatt gccggtggcg aaaaggccat
    15181 tattggcgtg atggtggaaa gccatctggt ggaaggcaat cagagcctcg agagcgggga
    15241 gccgctggcc tacggtaaga gcatcaccga tgcctgcatc ggctgggaag ataccgatgc
    15301 tctgttacgt caactggcga atgcagtaaa agcgcgtcgc gggtaaggtt taattgtcgg
    15361 atgcgccgtc agagtggcgt atccgatgaa tcaccacagg cctgataagt cgcgcagcgt
    15421 cgcatcaggc aatgtgctcc attgttagca acaaaaaagc cgactcactt gcagtcggct
    15481 ttctcatttt aaacgaatga cgtttacttc gctttaccct ggtttgcaac cgccgctgct
    15541 ttcgctgcga tctcgtcagc attacccaga taatagcgtt tcagcggttt gaaattctcg
    15601 tcgaactcat acaccagcgg cacgccagtc gggatattaa gctcaagaat ctcttcttcg
    15661 ctcatgttat caagatattt caccagcgca cgtaaagagt taccgtgtgc agcgatgatc
    15721 acgcgctcac cgctcttcat acgcggcaga atagtttcat tccagtaagg gatcacgcgg
    15781 tcaatggtca gcgccaggct ttccgtcagc ggcagttctt tctcgctcag tttcgcgtaa
    15841 cgcggatcgt gacccggata acgctcatca tctttagtca gttccggcgg agtcactgca
    15901 aaaccacgac gccactgttt cacctgctcg tcgccatact tttcagcagt ttccgctttg
    15961 ttcagaccct gcaacgcacc gtagtgacgt tcgttcagtt tccaggattt ctcaacgggc
    16021 agccatgcct gatccagttc gtccagcaca ttccacaggg tatggatagc gcgtttcagc
    16081 acagaagtgt aagcaaagtc aaagctgtaa ccttcctctt tcagcagctt acctgctgct
    16141 tttgcttcgc ttacgccttt ctcagacaga tccacgtcgt accaaccggt gaaacggttt
    16201 tctttgttcc actgactttc gccatgacga accagaacca gcttagttac agccatatac
    16261 ttactcctca aatcatcttt taatgataat aattctcatt atattgccgc gacgaagcaa
    16321 cagcaatgct tacgcataac catagcgaaa atagtggcgc agtgtaaggt tgttgtgaat
    16381 attgagttgc aaatatgtcg gtgtttgctg gtgatttgaa caatatgaga taaagccctc
    16441 atgacgaggg cttaacatta ctcagcaata aactgatatt ccgtcaggct ggaatactct
    16501 tcgccaggac gcaggaagca gtccggttgc ggccattcag ggtggttcgg gctgtccggt
    16561 agaaactcgc tttccagagc cagcccttgc cagtcggcgt aaggttcggt tccccgcgac
    16621 ggtgtgccgc cgaggaagtt gccggagtag aattgcagag ccggagcggt ggtgtagacc
    16681 ttcagctgca atttttcatc tgctgaccag acatgcgccg ccactttctt gccatcgcct
    16741 ttggcctgta acaagaatgc gtgatcgtaa cctttcactt tgcgctgatc gtcgtcggca
    16801 agaaactcac tggcgatgat tttggcgctg cggaaatcaa aagacgttcc ggcgacagat
    16861 ttcaggccgt cgtgcggaat gccgccttca tcaaccggca gatattcgtc cgccagaatc
    16921 tgcaacttgt gattgcgcac gtcagactgc tcgccgtcaa gattgaaata gacgtgatta
    16981 gtcatattca ccgggcaagg tttatcaact gtggcgcgat aagtaatgga gatacggtta
    17041 tcgtcggtca gacgatattg caccgtcgcg ccgagattac ccgggaagcc ctgatcacca
    17101 tcatctgaac tcagggcaaa cagcacctga cgatcgttct ggttcacaat ctgccagcga
    17161 cgtttgtcga acccttccgg cccgccgtgc agctggttaa cgccctgact tggcgaaagc
    17221 gtcacggttt caccgtcaaa ggtataacgg ctattggcga tacggttggc ataacgacca
    17281 atagaggccc ccagaaacgc ggcctgatcc tgatagcatt ccgggctggc acagccgagc
    17341 agcgcctcgc ggacgctgcc atcggaaagc ggaatacggg cggaaagtaa agtcgcaccc
    17401 cagtccatca gcgtgactac catccctgcg ttgttacgca aagttaacag tcggtacggc
    17461 tgaccatcgg gtgccagtgc gggagtttcg ttcagcactg tcctgctcct tgtgatggtt
    17521 tacaaacgta aaaagtctct ttaatacctg tttttgcttc atattgttca gcgacagctt
    17581 gctgtacggc aggcaccagc tcttccggga tcagcgcgac gatacagccg ccaaatccgc
    17641 cgccggtcat gcgtacgcca cctttgtcgc caatcacagc tttgacgatt tctaccagag
    17701 tgtcaatttg cggcacggtg atttcgaaat catcgcgcat agaggcatga gactccgcca
    17761 tcaactcgcc catacgtttc aggtcgcctt gctccagcgc gctggcagct tcaacggtgc
    17821 gggcgttttc agtcagtata tgacgcacgc gttttgccac gatcgggtcc agttcatgcg
    17881 caacagcgtt gaactcttca atggtgacat cacgcagggc tggctgctgg aagaaacgcg
    17941 caccggtttc gcactgttca cgacgggtgt tgtattcgct gccaaccagg gtacgtttga
    18001 agttactgtt gatgatgacg acagccacac ctttgggcat ggaaactgct ttggtcccca
    18061 gtgagcggca atcgatcagc aaggcatgat ctttcttgcc gagcgcggaa attagctgat
    18121 ccatgatccc gcagttacag cctacaaact ggttttctgc ttcctgaccg ttaagcgcga
    18181 tttgtgcgcc gtccagcggc agatgataaa gctgctgcaa tacggttccg accgcgactt
    18241 ccagtgaagc ggaagaactt aacccggcac cctgcggcac attgccgctg atcaccatgt
    18301 ccacgccgcc gaagctgttg ttacgcagtt gcagatgttt caccacgcca cgaacgtagt
    18361 tagcccattg atagttttca tgtgcgacaa tgggcgcatc gagggaaaac tcgtcgagct
    18421 gattttcata atcggctgcc atcacgcgaa ctttacggtc atcgcgtggt gcacaactga
    18481 tcacggtttg ataatcaatc gcgcagggca gaacgaaacc gtcgttgtag tcggtgtgtt
    18541 caccaatcaa attcacgcgg ccaggcgcct gaatggtgtg agtggcaggg tagccaaatg
    18601 cgttggcaaa cagagattgt gttttttctt tcagactcat ttcttacact ccggattcgc
    18661 gaaaatggat atcgctgact gcgcgcaaac gctctgctgc ctgttctgcg gtcaggtctc
    18721 gctgggtctc tgccagcatt tcataaccaa ccataaattt acgtacggtg gcggagcgca
    18781 gcagaggcgg ataaaagtgc gcgtgcagct gccagtgttg attctcttcg ccattaaatg
    18841 gcgcgccgtg ccagcccata gagtagggga aggagcactg gaagaggttg tcataacgac
    18901 tggtcagctt tttcaacgcc agcgccagat cgctgcgctg ggcgtcggtc aaatcggtga
    18961 tccgtaaaac gtgggctttg ggcagcagta gcgtttcgaa cggccaggca gcccagtaag
    19021 gcacgacggc taaccagtgt tcggtttcga caacggtacg gctaccgtct gccagctcgc
    19081 gctgaacata atccaccagc attggtgatt tctgttcggc aaaatattct ttttgcaggc
    19141 ggtcttcgcg ctcagcttcg ttaggcagga agctatttgc ccaaatctga ccgtgcggat
    19201 gcgggttaga gcagcccatc gccgcgcctt tgttttcaaa aacctgcacc catgggtacg
    19261 ttttccccag ttctgcggtt tgctcctgcc aggttttgac gatttccgtc aatgctgcaa
    19321 cgctgagctc tggcagcgtt ttactgtgat ccggtgaaaa gcagatcacc cggctggtgc
    19381 cgcgcgcgct ctggcaacgc atcagcggat cgtgactttc tggcgcatct ggcgtgtcag
    19441 acatcaaagc cgcaaagtca ttagtgaaaa cgtaagtccc ggtgtaatcg gggtttttat
    19501 cgcctgtcac ccgcacatta cctgcgcaga ggaagcaatc tggatcgtgc gcaggtaaca
    19561 cctgtttggc tggcgtttcc tgcgccccct gccaggggcg cttagcgcgg tgcggtgaaa
    19621 ccagaatcca ttgcccggtg agcgggttgt agcggcgatg tggatgatca acgggattaa
    19681 attgcgtcat ggtcgttcct taatcgggat atccctgtgg atggcgtgac tgccagtgcc
    19741 aggtgtcctg cgccatttca tcgagtgtgc gcgttacgcg ccagttcagt tcacggtcgg
    19801 ctttgctggc gtccgcccag taggccggaa ggtcgccctc gcgacgcggt gcaaaatgat
    19861 aattaaccgg tttgccgcag gctttgctga aggcattaac cacgtccagc acgctgttgc
    19921 ctacgccagc gccgaggttg tagatgtgta cgcctggctt gttcgccagt ttttccatcg
    19981 ccacgacgtg accgtccgcc agatccatta cgtggatgta atcgcgtacg ccagtaccat
    20041 cttcggtcgg ataatcgtta ccaaaaatcg ccagcgagtc gcgacggcct acagcaacct
    20101 gggcgatgta tggcatcagg ttattcggaa tgccttgcgg atcttcgccc atatcgcccg
    20161 acggatgcgc gccaaccggg ttgaagtagc gcagcagggc aatgctccag tccggctggg
    20221 ctttttgcag atcggtgagg atctgttcca ccatcagctt gcttttgccg taagggcttt
    20281 gcggtgtgcc ggtcgggaag ctttcaacgt atggaatttt gggctgatcg ccataaacgg
    20341 tggcggagga gctaaaaata aagtttttga cgttagcggc gcgcatggcg ctaatcaggc
    20401 gcagagtgcc gttgacattg ttgtcgtaat attccagcgg tttttgtacc gattcgccca
    20461 cggctttcag cccggcgaag tggatcacgg tgtcgatagc gtgatcgtgc aggatctcgg
    20521 tcatcaacgc ttcgttacga atatcgcctt caacaaacgt tggatgtttg ccgcctaaac
    20581 gctcgataac aggcagtacg ctgcgcttac tgttacagag gttatcaaga atgatgacat
    20641 catgaccgtt ttgcagtaat tgcacacagg tatgacttcc aatgtaaccg ctaccaccgg
    20701 taaccagaac tctcataatt cgctccatta ggcttatggt atgaaataac catagcataa
    20761 caaagatgcg aaaagtgtga catggaataa attagtggaa tcgtttacac aagaatttag
    20821 ccgtttttta tgcgcgatta agtgattata aaacagaggg tttatgaatg attgcgcttt
    20881 ttatctgaaa aaagacgcgg tttcatgcct gcatgcgtcg aaccgttggc cggagagggc
    20941 gctaaggccg cctccggcaa ggtcagcact accgactcaa tatatttttg tcagcacata
    21001 gcgatagagt ccaccgtccg gcacgaactc aagacgatgg gtaatacagg caggcgcatc
    21061 ttcagcgtgg tgcgaaacaa acaacaattg cgtttcacct tcgctaatca gcacatcaac
    21121 aaaacggcgg ataagctggc gattcagcgg atcaagcccc tgtagtggtt catcgagaat
    21181 aagcaacgtc ggatgtttca ccagtgcgcg gacaatcagc gccagacgct gctgtcccca
    21241 ggaaagacta tggaacggag cgtcagccgt gcgtttatca atgccgagaa tatccagcca
    21301 ctgctgcacc agtttttgct ggcgatccga aacggcctga taaatgccaa tcgaatcaaa
    21361 atagccagaa agaatcacat tacgcacggt agtgctgacc cggtaatcca gatgcaaact
    21421 actgctgacg taaccgatat gctttttgat atcccagatg gtttcgccgc tgccgcgacg
    21481 tcgtccgaaa agcgtcaaat cgttgctgta accttgcgga tgatcgccag taaccaggct
    21541 taataacgtc gattttcctg caccatttgg cccgacaatt tgccagtgtt cgcttggatt
    21601 cacctgccag ctaaggttat taagaatggg gcgatcgtta taagaaacca cgccattgtt
    21661 cagcacaatg cgcggttcgt tggcgggtaa ggcgtgacgt gctgaaggtt catccggctc
    21721 cggcagttgc acaccttcaa gctgttcact atgcgccagt tgcgcgacga gtgcttgttg
    21781 gagcagttcc tctttagcgc cagtttccgc taacgtgcaa tccgccagca cgccagcaaa
    21841 ctggacaaac tccgggatct catcgaagcg attgagcacc agtaccagag taataccgga
    21901 ctgatgtaac gaggcgagtc gctcagccag ctgctgacgt gaggcaacat ccaggccatc
    21961 gaacggctca tcaagaatca acaagtcagg ctccgacatc agcgcctgac acagcagggt
    22021 ttttcgcgtc tcgccagtgg aaaggtattt aaagcgtcgg tcgaggaggg cggtaatacc
    22081 gaactgctgc gccagttgca tgcaacgcgg tgcatccttt acttcatcct gaatgatctc
    22141 agccgtagtg cgtccggtgt catcttcgcc agggccgagc atatcggtgt tattccgctg
    22201 ccattcgtcg ctgacgagtt tttgcaattg ctcgaaggag agacgagtga tgtgggaaaa
    22261 ctggctttgc cgttcacctt tcaaaagcgg aagttccccc gccagcgcgc gggccagggc
    22321 cgatttcccg cttccattcg aaccgacaaa cgcccaacta tcacccgcgt ttaacgttag
    22381 ctgaggcaat tgcagcgttt ttgtgtcgct aagacgaaac gtgccttgca aaatttgcaa
    22441 cgatgacatt ttatatccca ctttgtgcag cgattactga cagggatacg tgtttcataa
    22501 caaattgtca acacgcttag cacagcgtgg cgataatcac gctgtcggca ttaaagtagg
    22561 ccgtgacatt ctgtccttgc tgaagagaag tcgcttcatt taccggcact gtggcgcaca
    22621 gtgtttgccc gtcgggtagc gccattaata cttcgcactg ctctgcgccg cgctcaatat
    22681 gactaataat acccggtaat tggttgtcag cgttttgcgc gaccgcctcg tcctgagtaa
    22741 tacctaccca cggcgctttt agcaatatca acacttcttt gccttcatcc agccccagac
    22801 gcgcgccgct ttgtgcggta attgcgactt tcaggcgtgt ttttccgtca gccagtaaga
    22861 catcaacatg ctgttgaacg tcatcatgat cgcgggcggt gatggtaccg aaccactggt
    22921 tacgggcgct ggtttgcagt gaaaaacgtg agatcgcggc cagcaggctg ttcagcggca
    22981 gggcgtcatc gtcacttaac acatcaaagg ctttttgctg gatttgcgcc agtaagtcat
    23041 agagctgaat cagtcgctga ccatagcggg tcagtactgc gccgccgcca cctttaccgc
    23101 ctgttgcgcg ctcgaccaga atatgctcac ttaactgatt catctcgtta atggcatccc
    23161 aggcgctttt atagctaata ccggcatctt tcgctccctg gctaatggaa ccggaaagcg
    23221 caatgtgttt tagtagcgaa atgcggcgcg ggtcggcgaa taatttttgt tggagcttaa
    23281 gggtgagaag gatttcggcc tgcataacaa tgtcctggca aaagtcttat tgtgacggaa
    23341 aacgaacgcc acgcaaagct gaccgcacaa aaggggagtg cttttctgtg cttagcggtt
    23401 agaatagtct catgactata tctggagttg accatgttag agttattaaa aagtctggta
    23461 ttcgccgtaa tcatggtacc tgtcgtgatg gccatcatcc tgggtctgat ttacggtctt
    23521 ggtgaagtat tcaacatctt ttctggtgtt ggtaaaaaag accagcccgg acaaaatcat
    23581 tgattccctg aatgcccgct tagtcgggca ttttcttttt ctcaacttcc tgcttttcct
    23641 gccgatattt tttcttatct acctcacaaa ggttagcaat aactgctggg aaaattccga
    23701 gttagtcgtt atattgtcgc ctacataacg ttacattaag gggttaccaa tggctcgtaa
    23761 atggttgaac ttgtttgccg gggcggcact ctctttcgct gttgctggca atgcactggc
    23821 agatgaaggg aaaatcacgg tgttcgccgc cgcatcactg actaacgcaa tgcaggacat
    23881 tgctacgcag tttaaaaaag agaaaggcgt ggatgtggtt tcttctttcg cttcgtcatc
    23941 tactctcgcc cgtcagattg aagcgggtgc gcctgcggat ctgtttattt ctgccgatca
    24001 gaaatggatg gattatgcgg ttgataaaaa agcgatcgat acagctacgc gtcagacact
    24061 gctcggcaat agcctggtcg ttgtagcacc gaaagccagc gtgcagaaag atttcaccat
    24121 cgacagcaaa accaactgga cttcactgct gaatggcggt cgcctggcgg ttggcgatcc
    24181 ggaacatgtt cccgctggca tttatgcaaa agaagcactg caaaaactgg gcgcatggga
    24241 tacgctctct ccgaaactgg ccccagcgga agatgttcgt ggggcgctgg cgctggtcga
    24301 acgtaacgaa gcgcctctgg gcattgtcta cggttctgac gcagttgcca gcaaaggggt
    24361 aaaagtggtt gccaccttcc cggaagattc acataaaaaa gtggaatatc cggttgctgt
    24421 tgtggaaggg cataacaatg cgacagtgaa agcattttat gattatctga agggaccgca
    24481 ggcagcggaa atctttaaac gttacggatt tacaatcaag taatgatact gaccgatcca
    24541 gaatggcagg cagttttatt aagcctgaaa gtttcttccc tggctgtgct gtttagcctg
    24601 ccgtttggga tcttttttgc ctggttactg gtgcgttgca cgtttccggg caaagctctg
    24661 ctcgacagcg tactgcatct accgctggtg ttaccgcccg tggtcgtcgg ttacttatta
    24721 ttagtttcga tgggacggcg cggatttatc ggtgaacgtc tgtatgactg gtttggtatt
    24781 accttcgcct ttagctggcg cggcgcggtt ctcgctgccg ccgtcatgtc gtttccgctg
    24841 atggtgcggg caattcgtct ggcgctggaa ggggttgatg tcaaactgga acaggccgca
    24901 agaacactgg gggccgggcg ctggcgcgtt ttctttacta tcacgttacc gctgacctta
    24961 ccgggaatta ttgttggtac ggtactggct tttgctcgtt ctctcggtga gtttggtgca
    25021 accatcacct ttgtgtcgaa cattcctggt gaaacgcgaa ccattccttc tgccatgtat
    25081 accctgatcc agacccccgg cggggaaagt ggagcggcga gactgtgcat tatttctatt
    25141 gcgctggcga tgatctccct gttgatttca gaatggctgg ccagaatcag ccgtgaacgg
    25201 gcggggcgct aatcatgctg gaactgaatt tttcccagac gttgggcaac cattgcctga
    25261 ctattaatga aacgctgccc gccaatggca tcactgctat ctttggcgtc tccggtgccg
    25321 gaaaaacttc gctgattaac gccatcagtg gactgacgcg cccgcaaaaa gggcggattg
    25381 tcctcaatgg gcgggtacta aatgatgccg aaaaaggtat ctgcctgacg ccggaaaagc
    25441 gtcgcgttgg ctatgttttt caggatgcgc ggctgttccc gcattacaaa gtgcgtggca
    25501 atctgcgcta cggcatgtcg aaaagtatgg tcgatcagtt cgataagctg gtggcgcttt
    25561 taggcattga accgttgctt gaccgtttac caggcagcct gtccggaggc gaaaaacagc
    25621 gcgtggcgat tggtcgggct ttgctgacag caccggaatt gctgttgctg gatgaaccgc
    25681 tggcgtcact ggatattccg cgtaaacgcg aactgttgcc ttatctgcaa cggctgacac
    25741 gggaaatcaa cattccgatg ttgtatgtca gccattcgct ggatgagatc ctccatctgg
    25801 cagacagagt gatggtactg gaaaacggtc aggtgaaagc ctttggcgcg ctggaggaag
    25861 tgtggggcag tagcgtgatg aatccgtggc tgccgaaaga gcaacaaagt agcattctga
    25921 aagtgacggt gcttgagcat catccgcatt acgcgatgac cgcgctggcg ctgggcgatc
    25981 agcatttgtg ggtcaataag ctggacgaac cgctgcaagc tgcgctacgt atccgcattc
    26041 aggcttccga tgtttctctg gttttacaac cgccgcagca aaccagcatt cgtaacgtat
    26101 tgcgggcaaa agttgttaat agttatgacg acaacggcca ggtggaagtg gaactggaag
    26161 tcggcggtaa aacgctgtgg gcgcgtatca gcccgtgggc cagggatgaa ctggcgatca
    26221 aacctggcct gtggctgtac gcgcaaatta aaagtgtgtc gataaccgcc tgattaaatc
    26281 aggtggctat aaatgaattg ggcaatgctg tcggtggtgt tatcaccaat cacaatgttg
    26341 gcgcgcgctt ttaccgcgtc atcggcgttg cccatcgcca cgcctgtacc agcggcttcc
    26401 agcatactga tatcattaaa gttatcgccg aatgccacga cgttttccat cgaccaacct
    26461 tgcgcctcaa cccatttcgt caaacgttta cctttgctgt tgccgccgcg tgcaatatca
    26521 acctgatcgt gccaggacca ttcacactcc agtcccagtt catgttcgac atgcttacca
    26581 aaatgctgca attgcggcag gtcatcgtgc gtcagggcga acttccatac ggcgttaact
    26641 tgttgcgccg tttcagccag agaagcgact tgtgtgaaag tcggacgctg ttccggcggc
    26701 agggtttgcg cccagttaga tgtgcgaatg acatgcccgg tcgggtgctc atagaccatt
    26761 gcatcatcga catacatcag accgtgaatg tggtgttcat tcagcatctc aatgagttgc
    26821 agggctttaa taacgggcat tgggtccgct tccagcacgg tttttgcatg ataatcatac
    26881 aaataggtgc cattacagca aatagcaggt gtatccagcg ccagcgcctg ataaaaagga
    26941 tgaatagcga cgtgatggcg acctgtgacg atgattaatt gatagcctgc ttcgcgagcg
    27001 cgggccaggg cttctatcga tgaaggaagc agggtctttt tcggggtcaa taaggtgccg
    27061 tctaagtcga gagcaatcac gcgtgtggtc atggcgtatt ccagattaag gttaagaatt
    27121 ttcgtctgcg cgaatggtac accgatacca ctatcggaca aaattctgcg ttttaattca
    27181 gcattcaccg ccaaaagcga ctaattttag ctgttacagt cagttgctaa atgcaaagga
    27241 gcattcatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg
    27301 aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg
    27361 cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt
    27421 cgcgtcctgg cgtatcgtat cgccccggac gatggcgcac tgacctttgc cgcagagtct
    27481 gcgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta
    27541 ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc
    27601 gtcgtcgatg tggtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac
    27661 cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat
    27721 gatggtcatc tcgtggcgca ggaccctgcg gaagtgacca ccgttgaagg ggccggcccg
    27781 cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc
    27841 tcagtggatg tctgggaact gaaagatccg cacggtaata tcgaatgtgt ccagacgctg
    27901 gatatgatgc cggaaaactt ctccgatacc cgttgggcgg ctgatattca tatcaccccg
    27961 gatgggcgtc acttatatgc ctgtgaccgt actgccagcc tgattactgt cttcagcgtt
    28021 tcggaagatg gcagcgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc
    28081 ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ctgggcaaaa atctcaccac
    28141 atctcggtat acgaaattgt tggcgagcag gggctactgc atgaaaaagg ccgctatgcg
    28201 gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttacccggcg
    28261 cagtctctcc tgcgccggtg tattaaccta tctcctgtaa cgcgtgtctc tggcgttcga
    28321 cgatattggt ccacaaattg tctttatcgt cagtccataa attaatcagc aaggcaaaaa
    28381 agcgttctgc tgctggagaa agtacggcat ctttacggcg aattattccc aatgttcgac
    28441 gtataacggg ttccaccaat gggataccaa taatcgaaga atagggcgca tgaggcattg
    28501 ccaggccagg aagcgccgaa atccccagtc ccgcctccac cagtcctaat gacgtcgaaa
    28561 gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcggccagc tgttgttcta
    28621 tcaacagtcg gttgccgctg gaggagcgta cgccaatcat tttgtaaccc actaactcct
    28681 gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggcc agcacgaacg
    28741 gctcgttgac cagtggggta aaatcaatgg atgaatttgt gacgttgttc atgtttatgc
    28801 caaagtctga ttcgttacac agcaccgatt ccatgcaatt attcgtgcct tgttccagaa
    28861 tccgcacttt aatattggga tacagctcat taaatttacc gattgccagc ggtaaaaaat
    28921 aaaatactgc ggtcggaata cacgctaacg tcaccatacc acgatgataa gcattcatat
    28981 cacgaatatt aaaaagcgtt tcatcgaatt tctttattaa ttcctgcgcc tccgggagca
    29041 acctttttcc tgcttttgtt aacgtaacct tgcgagttgt acgctcaaat agttgaatat
    29101 gtaaatcttc ctccatcttt tttattcttc gcgttaatgc aggttgcgta atattgagta
    29161 atttagcggc attgttaaac gaactggact ctgccagtat gacaaatgcc ttcatacttg
    29221 ataattcatg cttcattatt actccggaaa atggaagcga cgattttggg tggctggccg
    29281 ttaaaaattt taactgcatt tagccaactt aaattaatga aaaaatgtta ttaatcgttg
    29341 agctaaagtc attagagatg ctttgccctt aatgtaacca tatcgcaata agttatgttt
    29401 ttaaattgag ggcattatta tgaaaaaaat accctgcgtg atgatgcgag gtggaacctc
    29461 gaggggcgcg ttcctgttag cggaacattt acccgaagat caaacgcagc gcgataaaat
    29521 attgatggca attatgggtt ccggtaacga tctggaaatt gacggtattg gcggcggtaa
    29581 tccactgacc agtaaagtcg ccattattag ccgttccagc gatccgcgtg ctgatgtcga
    29641 ttatctgttt gcgcaggtaa tcgttcatga gcaacgtgtc gataccacgc ctaactgcgg
    29701 caatatgctg tctggtgttg gggcattcgc cattgaaaat ggtttgattg cagcgacttc
    29761 gccagttact cgcgtacgta tccgcaacgt caatacgggt acgttcatcg aagctgatgt
    29821 gcaaacgcca aatggtgttg tcgagtacga gggtagcgcc agaattgacg gcgtaccggg
    29881 tactgccgca ccggttgcgc tcactttcct gaatgccgct ggaaccaaaa ccggaaaagt
    29941 tttcccgact gataatcaga ttgattattt tgacgatgtc ccggtgacct gtatcgatat
    30001 ggcgatgcca gtcgtcatta ttccggctga atatctggga aaaacaggtt atgaattacc
    30061 ggcggaactg gatgccgaca aagcattatt agcccgcatt gaatctatcc gtctacaagc
    30121 gggtaaagca atgggcttag gtgatgtcag taatatggtt atccctaaac ctgtgcttat
    30181 ttctccagcg cagaaaggcg gggcaattaa tgtgcgttat tttatgccgc attcttgcca
    30241 tcgcgcgctg gcgataaccg gtgctattgc tatttccagt agttgtgcat tggaaggcac
    30301 cgtcacccga caaatcgtcc cttctgtagg atacggcaat atcaatattg aacaccccag
    30361 tggtgcgctc gacgttcatt taagtaatga aggtcaggat gccacgacgt tacgcgcatc
    30421 tgttattcgg acgaccagaa aaatattttc cggtgaagtt tatcttccct gaaaaaattc
    30481 gttgtcagga taaggacaat caataaagga cttctgtatg agtcatacag aaagaacagg
    30541 attttaaatg aataagaaat cgttatggaa gctaattctg atattagcga tcccatgtat
    30601 tattggcttt atgccagctc cggcaggatt aagcgaactg gcgtgggtgc tttttggtat
    30661 ttacctggcg gccattgtgg gtctggttat caagcctttc ccggaacctg tcgtactgtt
    30721 aattgccgtt gctgcctcaa tggtggtggt cggtaactta tccgacggtg cgtttaagac
    30781 caccgccgta ttaagcggtt actcttcagg tactacctgg ctggtgttct cggcgtttac
    30841 cttaagcgcc gcatttgtga ccaccggttt aggtaaacgt attgcctatc tgctgattgg
    30901 taaaatcggt aacaccacgc tgggtctggg ttacgttacg gtattcctcg atctggtact
    30961 ggctccggca acaccgtcta acaccgcgcg tgcgggcggc attgtgttac cgatcatcaa
    31021 cagcgtggcg gtggctttgg ggtccgaacc ggaaaaaagt ccgcgtcgtg tcggacatta
    31081 cctgatgatg tccatttaca tggtcaccaa aaccaccagc tatatgttct ttaccgcaat
    31141 ggcggggaac attctggcgc tgaaaatgat caacgacatt ctgcacctgc aaattagctg
    31201 gggtggatgg gcgctggccg ccggattgcc gggcatcatt atgctgctgg tcaccccgct
    31261 ggtaatttac accatgtatc caccagaaat taaaaaggtg gataacaaaa ccatcgctaa
    31321 agcgggcctt gccgaactgg gaccgatgaa aatccgcgaa aaaatgctgc tcggtgtctt
    31381 tgtgctggcg ctactgggct ggattttcag taagtctctg ggggttgatg aatccactgt
    31441 ggcaatcgtt gttatggcga ccatgctgct gctgggtatc gttacctggg aagacgtggt
    31501 taaaaataaa ggcggctgga ataccttaat ctggtacggc ggtattatcg gattaagctc
    31561 cttattatcg aaagttaaat tcttcgaatg gttagctgaa gtctttaaaa ataacctggc
    31621 atttgatggt cacggtaacg ttgctttctt cgttattatt ttcctcagca ttatcgtgcg
    31681 ttatttcttc gcttccggta gtgcctatat cgttgctatg ttaccggtat ttgccatgct
    31741 ggcgaacgtc tccggcgcac cgttaatgtt aaccgcgctg gcactgttgt tctccaactc
    31801 ctatggcggc atggttactc actatggcgg cgcggcaggt ccggtcatct ttggcgtggg
    31861 ttacaacgat attaaatcct ggtggttggt cggtgcggta ctgacgatat taaccttcct
    31921 ggtgcatatc accctcggcg tgtggtggtg gaatatgctg atcggctgga acatgctgta
    31981 aatattctcg tcatacttca agttgcatgt gctgcgtctg cgttcgctca ccccagtcac
    32041 ttacttatgt aagctcctgg ggattcactc gcttgtcgcc ttcctgcaac tcgaattatt
    32101 tagagtatat ccatttatta tctttctgcg cacttcacgg tgcgcagata tctggagcat
    32161 ttgatgatca agttatctga aaaaggcgtg tttctcgcca gtaataacga aataattgcc
    32221 gaagaacatt tcaccggcga aattaaaaaa gaagaagcca aaaaaggcac tattgcctgg
    32281 tctattctct cttcgcataa tacgtccgga aatatggata aacttaaaat taagtttgat
    32341 tcattagcct ctcacgatat tacctttgtt ggtattgtac agaccgctaa agcgtccggt
    32401 atggaacgtt tcccgctgcc gtatgtgctg accaactgcc ataactcact ctgcgccgtc
    32461 ggcggcacta ttaacggtga tgaccatgtt tttggtttat cggcggccca gcgttatggc
    32521 ggtatttttg tgcctccgca tattgcggtc atccatcaat atatgcgtga gatgatggca
    32581 ggcggcggca aaatgatcct cgggtcagac agccacaccc gttacggtgc attagggaca
    32641 atggcagtcg gtgagggcgg cggtgagttg gtaaaacagc tgcttaatga cacctgggat
    32701 atcgactatc cgggcgtggt tgcggtgcat ctgaccggaa aacctgcgcc gtatgtgggg
    32761 ccacaggatg tggcgctggc tatcattggc gcggtgttca aaaacggtta cgtcaaaaac
    32821 aaagtcatgg agttcgttgg accgggcgtt agcgcgctct ctaccgattt ccgtaacagc
    32881 gttgacgtga tgaccactga aacgacctgt ttaagttctg tctggcaaac cgatgaagaa
    32941 gtccataact ggctggcgct gcacggtcgc ggccaggatt actgccagct taaccctcaa
    33001 ccgatggcgt actacgatgg ctgcatcagc gttgatttaa gcgccatcaa accaatgatt
    33061 gcgctgccgt tccacccgag caacgtgtat gaaatcgaca cactgaacca gaacctgacc
    33121 gacattctgc gtgagattga aattgagtcc gaacgcgtgg cgcacggtaa agccaaactc
    33181 tcgctgctgg ataaagtgga aaatggtcgc ctgaaagtgc agcaggggat tatcgcgggc
    33241 tgttctggcg gtaactacga aaacgtcatc gcggcggcga atgcactgcg cggtcaatcc
    33301 tgtggcaatg acaccttctc gctggcagtt tacccgtcat cacagccggt gtttatggat
    33361 ctcgccaaaa aaggtgtggt agcagatttg attggcgcag gcgcaatcat cagaaccgcg
    33421 ttctgcggcc catgctttgg cgcgggcgat acgccaatca acaacggttt gagtattcgc
    33481 cacaccacgc gtaacttccc gaaccgcgaa ggctctaagc cagctaatgg gcagatgtca
    33541 gcggtggcgt tgatggacgc tcgttctatc gctgcgactg cggcaaacgg tggctattta
    33601 acctctgcca gcgaacttga ttgctgggac aacgtgccgg agtacgcctt cgatgtaacg
    33661 ccgtataaaa accgtgttta tcagggcttt gtgaaagggg caactcagca accgctgatt
    33721 tacgggccga acattaaaga ctggccggaa ttgggtgcgc tgactgacaa tatcgtcctg
    33781 aaagtgtgct cgaagatcct cgacgaagtg accaccaccg acgaactgat tccttccggt
    33841 gaaacctctt cttatcgttc aaatccgatt ggtctggcgg agtttaccct gtctcgccgc
    33901 gatcccggtt atgttagcag aagtaaagcg actgctgagc tggaaaatca gcgtctggcg
    33961 gggaatgtca gcgagctgac agaggtgttt gcgcgcatta agcagattgc tggtcaggag
    34021 catattgatc cgctgcaaac tgaaattggc agcatggtct atgcggtgaa accaggcgat
    34081 ggttctgcgc gtgaacaggc ggcgagctgc cagcgtgtga ttggcggtct ggcgaatatt
    34141 gccgaggagt acgcgactaa acgctatcgt tctaacgtca tcaactgggg gatgttaccg
    34201 ctgcaaatgg cggaagtacc aacctttgaa gtgggggatt acatttacat ccctggcatt
    34261 aaagcggcgc tggataatcc gggtacgacg tttaaaggtt atgtgatcca tgaagatgcg
    34321 ccggtaacgg aaattacgct ctatatggaa agtctgactg ctgaagagcg cgagattatc
    34381 aaggcgggta gtttgattaa cttcaataaa aaccgtcaga tgtaaaaagc gccatgtgaa
    34441 tgtaggtcgc attcggcact tattgtcgga tgcgatgctt gcgcatctta tccgacctac
    34501 gaatcgcatc gaatctgtag gccagataag gcattttcgc agcatccggc acttattgtc
    34561 ggatgcgatg cttgcgcatc ttatccgacc tacaaatcgc atcgaaccgt aggccggata
    34621 aggcgtttac gccgcatccg gcaaatagtt aattgctctt acttcttcgc ctctgcaacc
    34681 actttactac ccacgccgcg gttattgtat tcccacatgc ggttgtagtt agtgtcattc
    34741 agattgcgct gtatttcgtc gttatcatct acgctgccgg tattacccgc aaacggacga
    34801 ttagagatca ccgcatcggc ccacggttta gccgtgttaa aaccttcgtt gatggcgcta
    34861 tcacggatca ccacctgacc gttggtattg gcatcaacat ccagcgagcg gcccagttgc
    34921 gccacaccat caccgaaagc attgaaacgg ctgtttacgg cgaggaaacc gtagtaaatg
    34981 ttggacagcg tagccggtgc aaacacatac gcttcttgct gagtacgtga gttcaccacg
    35041 cggaattcgg tgttatcgaa caccactgcg ccgcgaccag aaacgatatc cacatcccct
    35101 tcaatgtagc tgttggtcac cagcgtacgc ggctgacgat tcgtttccag acggttctgc
    35161 acaccgctgt tggtgacaaa gaaggtgttc tgacgaccga gaatgttaac gttgttaatc
    35221 tgtacctggt caccatcagt acgcagtgcc accgccggat ggttacctgc atctacgcta
    35281 tcgcccagcg tgttttcgat ggtcagattt tgcagttgca ggccattgtt ttgtgaccag
    35341 aagaccgcag agcagagaac accgatactg tcgctgcgtt tgctctggca gctatcgtac
    35401 atataccacg ctggtttacc tggcatatat ttgccgcgcg ggttgacgtc gtgacgccag
    35461 tcggcagggc tcatgccacc atcaagggaa agcccaatct tcacatcaat cggtttttca
    35521 cctgtaccgt acagagtaat tccacccgga gcggcaggga catataccgt tccctgatac
    35581 tcaccaggca tcacggcaat atactggcgc ttgttggtac gcttgataat tgccgcatct
    35641 accgccgcct gaatcgtggt atgcgttaca ccttgagtgc ccgccgggcc gacaacaaag
    35701 tcaggttgcg caggcagggt aatcggggaa ggattccacg ctgcagcacc tggtgtcagg
    35761 gatgcaaaat agtgttgagc atcgaaattc tgcgcttctt ttgccgacag aatcgggcga
    35821 gaagaggtac caggcgcggt ttgatcagaa ggacgttgat cgggcggggt tgagctacag
    35881 gcggtcagcg tcacgccaaa agccaatgcc agcgccagac gggaaactga aaatgtgttc
    35941 acaggttgct ccgggctatg aaatagaaaa atgaatccgt tgaagcctgc ttttttatac
    36001 taacttgagc gaaacgggaa ggtaaaaaga caaaaagttg tttttaatac ctttaagtga
    36061 taccagatgg cattgcgcca tctggcagag tgattaacta aacatcgcag taatcgaggc
    36121 gcttgccaga gagtggaaat gaacgttaaa cccgaccatc gcgccgctgg caccttcatc
    36181 gacatcaata cgttctatat ccagcgcgtg aacggtaaaa atgtagcgat gagtttcgcc
    36241 tttcggcggt gctgcgccat cgtacccggt tttaccaaag tcggtacgcg tctgcaaaac
    36301 gccgtctggc attgctacca gaccagagcc aaacccttgc ggtaatacgc gggtatcagc
    36361 gggtaagtta acaactaccc agtgccacca gccggagccg gttggcgcat ccgggtcgta
    36421 gcaggtgaca acaaaacttt tcgttcccgc aggaacatca tcccacgcca gatgcggtga
    36481 aatattatcg ccatcgtaac ccatgccgtt aaagacatga cgatgcggca atttatcgcc
    36541 atcgcgcaga tcgttactga tgagtttcat gaaccctcct ttcttgtttg cagaaagtgt
    36601 agccagaaac cctcacgcgg acttcccgtt attggcaaaa aaatgtttca tcctgtaccg
    36661 cgcggttaac cgctgcggtc agacgctgca actgttgcgg gagaataata tagggcggca
    36721 tcaggtaaat cagtttgcca aaaggccgga tccagacacc ctgttcgaca aagaattttt
    36781 gcagcgccgc catattcacc ggacgagtgg tttcgaccac accgattgcc cccagtacgc
    36841 gcacatcggc aaccatttcg gcatcacggg ctggtgccag ttgctcgcgc agctgcactt
    36901 caatagccgc cacctgctgc tgccattcgc cggattcgat aatcgccagg ctggcgtttg
    36961 ctgccgcgca ggccagcgga ttgcccataa aagttggccc atgcataaag cagccggctt
    37021 cgccgttact gatggtttct gcaacctcgc gcgtggtaag tgtggcggaa agggtcattg
    37081 tgccgccggt taaggcttta ccgaggcaca aaatgtccgg cgcgatttct gcatattcac
    37141 aggcaaacag tttgccggta cgaccaaatc cggtggcgat ctcgtcggca atcagcaaga
    37201 taccttcgcg atcgcacatt ttgcggattc gttttaacca ttccggatgg tacatgcgca
    37261 tcccgcctgc gccctggaca atcggctcaa tgatcaccgc cgcgatttca tgacgatgcg
    37321 ccgccatcag gcgggcaaag cccaccatat cgcgctcatc ccattcgcca tccatgcggc
    37381 tttgcggggc gggagcaaac aggttttctg gcaggtagcc tttccacaga ctgtgcattg
    37441 agttatccgg atcgcacacc gacatcgcgc caaaggtatc gccatgataa ccattgcgga
    37501 aggtcagaaa acgctggcgc gcttcgcctt tggcttgcca gtactgcaac gccattttca
    37561 tcgccacttc caccgctacg gaaccggagt ccgcgagaaa aacgcactcc agcggttgcg
    37621 gcgtcatcgc caccagtttg cggcacagct caatggctgg cgcatgggtg ataccgccaa
    37681 acatcacatg cgacatggca tcaatttgcg acttcatcgc cgcattaagc tgcgggtgat
    37741 tgtagccgtg gatcgccgcc caccaggacg acataccgtc aaccaggcgt ctgccgtcag
    37801 acaaaatcag ctcgcaacct tcggcgctca ccaccggata aaccggcaga ggggaggtca
    37861 tggatgtgta tgggtgccag atatggcgtt ggtcaaaggc aagatcgtcc gttgtcataa
    37921 tcgacttgta aaccaaattg aaaagattta ggtttacaag tctacaccga attaacaaca
    37981 aaaaacacgt tttggagaag ccccatggct caccgcccac gctggacatt gtcgcaagtc
    38041 acagaattat ttgaaaaacc gttgctggat ctgctgtttg aagcgcagca ggtgcatcgc
    38101 cagcatttcg atcctcgtca ggtgcaggtc agcacgttgc tgtcgattaa gaccggagct
    38161 tgtccggaag attgcaaata ctgcccgcaa agctcgcgct acaaaaccgg gctggaagcc
    38221 gagcggttga tggaagttga acaggtgctg gagtcggcgc gcaaagcgaa agcggcagga
    38281 tcgacgcgct tctgtatggg cgcggcgtgg aagaatcccc acgaacgcga tatgccgtac
    38341 ctggaacaaa tggtgcaggg ggtaaaagcg atggggctgg aggcgtgtat gacgctgggc
    38401 acgttgagtg aatctcaggc gcagcgcctc gcgaacgccg ggctggatta ctacaaccac
    38461 aacctggaca cctcgccgga gttttacggc aatatcatca ccacacgcac ttatcaggaa
    38521 cgcctcgata cgctggaaaa agtgcgcgat gccgggatca aagtctgttc tggcggcatt
    38581 gtgggcttag gcgaaacggt aaaagatcgc gccggattat tgctgcaact ggcaaacctg
    38641 ccgacgccgc cggaaagcgt gccaatcaac atgctggtga aggtgaaagg cacgccgctt
    38701 gccgataacg atgatgtcga tgcctttgat tttattcgca ccattgcggt cgcgcggatc
    38761 atgatgccaa cctcttacgt gcgcctttct gccggacgcg agcagatgaa cgaacagact
    38821 caggcgatgt gctttatggc aggcgcaaac tcgattttct acggttgcaa actgctgacc
    38881 acgccgaatc cggaagaaga taaagacctg caactgttcc gcaaactggg gctaaatccg
    38941 cagcaaactg ccgtgctggc aggggataac gaacaacagc aacgtcttga acaggcgctg
    39001 atgaccccgg acaccgacga atattacaac gcggcagcat tatgagctgg caggagaaaa
    39061 tcaacgcggc gctcgatgcg cggcgtgctg ccgatgccct gcgtcgccgt tatccggtgg
    39121 cgcaaggagc cggacgctgg ctggtggcgg atgatcgcca gtatctgaac ttttccagta
    39181 acgattattt aggtttaagc catcatccgc aaattatccg tgcctggcag cagggggcgg
    39241 agcaatttgg catcggtagc ggcggctccg gtcacgtcag cggttatagc gtggtgcatc
    39301 aggcactgga agaagagctg gccgagtggc ttggctattc gcgggcactg ctgtttatct
    39361 ctggtttcgc cgctaatcag gcagttattg ccgcgatgat ggcgaaagag gaccgtattg
    39421 ctgccgaccg gcttagccat gcctcattgc tggaagctgc cagtttaagc ccgtcgcagc
    39481 ttcgccgttt tgctcataac gatgtcactc atttggcgcg attgcttgct tccccctgtc
    39541 cggggcagca aatggtggtg acagaaggcg tgttcagcat ggacggcgat agtgcgccac
    39601 tggcggaaat ccagcaggta acgcaacagc acaatggctg gttgatggtc gatgatgccc
    39661 acggcacggg cgttatcggg gagcaggggc gcggcagctg ctggctgcaa aaggtaaaac
    39721 cagaattgct ggtagtgact tttggcaaag gatttggcgt cagcggggca gcggtgcttt
    39781 gctccagtac ggtggcggat tatctgctgc aattcgcccg ccaccttatc tacagcacca
    39841 gtatgccgcc cgctcaggcg caggcattac gtgcgtcgct ggcggtcatt cgcagtgatg
    39901 agggtgatgc acggcgcgaa aaactggcgg cactcattac gcgttttcgt gccggagtac
    39961 aggatttgcc gtttacgctt gctgattcat gcagcgccat ccagccattg attgtcggtg
    40021 ataacagccg tgcgttacaa ctggcagaaa aactgcgcca gcaaggctgc tgggtcacgg
    40081 cgattcgccc gccaaccgta cccgctggta ctgcgcgact gcgcttaacg ctaaccgctg
    40141 cgcatgaaat gcaggatatc gaccgtctgc tggaggtgct gcatggcaac ggttaataaa
    40201 caagccattg cagcggcatt tggtcgggca gccgcacact atgagcaaca tgcagatcta
    40261 cagcgccaga gtgctgacgc cttactggca atgcttccac agcgtaaata cacccacgta
    40321 ctggacgcgg gttgtggacc tggctggatg agccgccact ggcgggaacg tcacgcgcag
    40381 gtgacggcct tagatctctc gccgccaatg cttgttcagg cacgccagaa ggatgccgca
    40441 gaccattatc tggcgggaga tatcgaatcc ctgccgttag cgactgcgac gttcgatctt
    40501 gcatggagca atctcgcagt gcagtggtgc ggtaatttat ccacggcact ccgcgagctg
    40561 tatcgggtgg tgcgccccaa aggcgtggtc gcgtttacca cgctggtgca gggatcgtta
    40621 cccgaactgc atcaggcgtg gcaggcggtg gacgagcgtc cgcatgctaa tcgcttttta
    40681 ccgccagatg aaatcgaaca gtcgctgaac ggcgtgcatt atcaacatca tattcagccc
    40741 atcacgctgt ggtttgatga tgcgctcagt gccatgcgtt cgctgaaagg catcggtgcc
    40801 acgcatcttc atgaagggcg cgacccgcga atattaacgc gttcgcagtt gcagcgattg
    40861 caactggcct ggccgcaaca gcaggggcga tatcctctga cgtatcatct ttttttggga
    40921 gtgattgctc gtgagtaaac gttattttgt caccggaacg gataccgaag tggggaaaac
    40981 tgtcgccagt tgtgcacttt tacaagccgc aaaggcagca ggctaccgga cggcaggtta
    41041 taaaccggtc gcctctggca gcgaaaagac cccggaaggt ttacgcaata gcgacgcgct
    41101 ggcgttacag cgcaacagca gcctgcagct ggattacgca acagtaaatc cttacacctt
    41161 cgcagaaccc acttcgccgc acatcatcag cgcgcaagag ggcagaccga tagaatcatt
    41221 ggtaatgagc gccggattac gcgcgcttga acaacaggct gactgggtgt tagtggaagg
    41281 tgctggcggc tggtttacgc cgctttctga cactttcact tttgcagatt gggtaacaca
    41341 ggaacaactg ccggtgatac tggtagttgg tgtgaaactc ggctgtatta atcacgcgat
    41401 gttgactgca caggtaatac aacacgccgg actgactctg gcgggttggg tggcgaacga
    41461 tgttacgcct ccgggaaaac gtcacgctga atatatgacc acgctcaccc gcatgattcc
    41521 cgcgccgctg ctgggagaga tcccctggct tgcagaaaat ccagaaaatg cggcaaccgg
    41581 aaagtacata aaccttgcct tgttgtagcc attctgtatt tggttaaatt gcgagcgaga
    41641 tcgcgtcttc gattgactgc aatttaacca attaaattct aaaataatca cgaaaaaaat
    41701 tttacttccg cctcatgcgg cgaatgtggg aattgcccag gcggcggggg ataggggctg
    41761 gagacagtta tcca
//
LOCUS       DAJOSY010000026        40860 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJOSY010000026 DAJOSY010000000
VERSION     DAJOSY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.026"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(110..2101)
                     /gene="tkt"
                     /locus_tag="OHN27_001553"
     CDS             complement(110..2101)
                     /gene="tkt"
                     /locus_tag="OHN27_001553"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCQ2286013.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2379..3137
                     /gene="loiP"
                     /locus_tag="OHN27_001554"
                     /gene_synonym="yggG"
     CDS             2379..3137
                     /gene="loiP"
                     /locus_tag="OHN27_001554"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCQ2286014.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKALSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGNLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3343..4263)
                     /gene="speB"
                     /locus_tag="OHN27_001555"
     CDS             complement(3343..4263)
                     /gene="speB"
                     /locus_tag="OHN27_001555"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCQ2286015.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4401..6377)
                     /gene="speA"
                     /locus_tag="OHN27_001556"
     CDS             complement(4401..6377)
                     /gene="speA"
                     /locus_tag="OHN27_001556"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCQ2286016.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGIFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6386..6517)
                     /gene="yqgB"
                     /locus_tag="OHN27_001557"
     CDS             complement(6386..6517)
                     /gene="yqgB"
                     /locus_tag="OHN27_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCQ2286017.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            6653..6868
                     /locus_tag="OHN27_001558"
     CDS             6653..6868
                     /locus_tag="OHN27_001558"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286018.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            7172..8326
                     /gene="metK"
                     /locus_tag="OHN27_001559"
     CDS             7172..8326
                     /gene="metK"
                     /locus_tag="OHN27_001559"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCQ2286019.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            8750..10144
                     /gene="galP"
                     /locus_tag="OHN27_001560"
     CDS             8750..10144
                     /gene="galP"
                     /locus_tag="OHN27_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCQ2286020.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            10221..10718
                     /locus_tag="OHN27_001561"
     CDS             10221..10718
                     /locus_tag="OHN27_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCQ2286021.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            10813..11520
                     /gene="endA"
                     /locus_tag="OHN27_001562"
     CDS             10813..11520
                     /gene="endA"
                     /locus_tag="OHN27_001562"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCQ2286022.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            11600..12331
                     /gene="rsmE"
                     /locus_tag="OHN27_001563"
     CDS             11600..12331
                     /gene="rsmE"
                     /locus_tag="OHN27_001563"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCQ2286023.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQLDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            12344..13294
                     /gene="gshB"
                     /locus_tag="OHN27_001564"
     CDS             12344..13294
                     /gene="gshB"
                     /locus_tag="OHN27_001564"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCQ2286024.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            13403..13966
                     /locus_tag="OHN27_001565"
     CDS             13403..13966
                     /locus_tag="OHN27_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCQ2286025.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKK
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            13966..14382
                     /gene="ruvX"
                     /locus_tag="OHN27_001566"
     CDS             13966..14382
                     /gene="ruvX"
                     /locus_tag="OHN27_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCQ2286026.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(14566..15546)
                     /locus_tag="OHN27_001567"
     CDS             complement(14566..15546)
                     /locus_tag="OHN27_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCQ2286027.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREGPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHRVGEGRL"
     gene            15564..16268
                     /gene="yggS"
                     /locus_tag="OHN27_001568"
     CDS             15564..16268
                     /gene="yggS"
                     /locus_tag="OHN27_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCQ2286028.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16286..16852
                     /gene="yggT"
                     /locus_tag="OHN27_001569"
     CDS             16286..16852
                     /gene="yggT"
                     /locus_tag="OHN27_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCQ2286029.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            16849..17139
                     /locus_tag="OHN27_001570"
     CDS             16849..17139
                     /locus_tag="OHN27_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCQ2286030.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            17147..17740
                     /locus_tag="OHN27_001571"
     CDS             17147..17740
                     /locus_tag="OHN27_001571"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCQ2286031.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            17733..18869
                     /gene="hemW"
                     /locus_tag="OHN27_001572"
     CDS             17733..18869
                     /gene="hemW"
                     /locus_tag="OHN27_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCQ2286032.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(19024..20031)
                     /locus_tag="OHN27_001573"
     CDS             complement(19024..20031)
                     /locus_tag="OHN27_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCQ2286033.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(20148..21194)
                     /gene="ansB"
                     /locus_tag="OHN27_001574"
     CDS             complement(20148..21194)
                     /gene="ansB"
                     /locus_tag="OHN27_001574"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCQ2286034.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(21370..22089)
                     /locus_tag="OHN27_001575"
     CDS             complement(21370..22089)
                     /locus_tag="OHN27_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCQ2286035.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(22273..22599)
                     /locus_tag="OHN27_001576"
     CDS             complement(22273..22599)
                     /locus_tag="OHN27_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCQ2286036.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(22599..23318)
                     /gene="trmB"
                     /locus_tag="OHN27_001577"
     CDS             complement(22599..23318)
                     /gene="trmB"
                     /locus_tag="OHN27_001577"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCQ2286037.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            23479..24531
                     /gene="mutY"
                     /locus_tag="OHN27_001578"
     CDS             23479..24531
                     /gene="mutY"
                     /locus_tag="OHN27_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCQ2286038.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            24559..24834
                     /locus_tag="OHN27_001579"
     CDS             24559..24834
                     /locus_tag="OHN27_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCQ2286039.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            24899..25978
                     /gene="mltC"
                     /locus_tag="OHN27_001580"
     CDS             24899..25978
                     /gene="mltC"
                     /locus_tag="OHN27_001580"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCQ2286040.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            26180..27436
                     /gene="nupG"
                     /locus_tag="OHN27_001581"
     CDS             26180..27436
                     /gene="nupG"
                     /locus_tag="OHN27_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCQ2286041.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(27486..29621)
                     /gene="speC"
                     /locus_tag="OHN27_001582"
     CDS             complement(27486..29621)
                     /gene="speC"
                     /locus_tag="OHN27_001582"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCQ2286042.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPYR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVEIGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLVE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGVKRLYGYVLK"
     gene            30019..30726
                     /locus_tag="OHN27_001583"
     CDS             30019..30726
                     /locus_tag="OHN27_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCQ2286043.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            30832..30907
                     /locus_tag="OHN27_001584"
     tRNA            30832..30907
                     /locus_tag="OHN27_001584"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30865..30867,aa:Phe,seq:gaa)
     gene            31084..32268
                     /locus_tag="OHN27_001585"
     CDS             31084..32268
                     /locus_tag="OHN27_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2286044.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQATDILLQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSIQ
                     ES"
     gene            32418..33428
                     /locus_tag="OHN27_001586"
     CDS             32418..33428
                     /locus_tag="OHN27_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCQ2286045.1"
                     /translation="MSLKAIATALGVSVTTVSRALGGYADVSASTRARVEAEARRRGY
                     RPNTQARRLKTGKTDAVGLVYPGRDVPFNSGVFMDMVSCISRELAHHDIDLLLIADDE
                     HADCHSYMRLVESRRVDALIIAHTLDDDPRITHLHKAGIPFLALGRVPSGLPCAWFDF
                     DNHAGTGQATQKLIALGHKRIALLSEDTSHSYVVARRQGWLDALNEHELEDTWLRLVS
                     PTRRAGYQAVMELMSLPIPPTAIITDNDLSGDGAAMALQLSGRLSGKDAVSLVVYDGL
                     PQDSIIELDVAAVIQSTRSLVGRQISEMIYQIITDSSSKPLQVVWNPVFYPGKTIHPP
                     CS"
     gene            33524..35650
                     /locus_tag="OHN27_001587"
     CDS             33524..35650
                     /locus_tag="OHN27_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCQ2286046.1"
                     /translation="MVSKYCRLSSPRSDLIIKTRPHAEIIWWGSALKHFSPDDCASLE
                     RPVANGRLDIDTPLTLMAENALGLFSSPGLEGHRNGLDASPVFYTVDVEHTENTLRLT
                     SEDSVAGLRLVSELVMTPSGILKVRHALTNLREGDWQINRFAITLPLAERAEEVMAFH
                     GRWTREFQPHRVRLTHDAFVLENRRGRTSHEHFPALIVGTPGFSEQQGEVWAVHLGWS
                     GNHRMRCEAKTDGRRYVQAEALWMPGEKALRKNETLYTPWLYACHSADGLNGMSQQYH
                     RFLRDEIIRFPEQKPRPVHLNTWEGIYFNHNPDYIMQMAERAAALGVERFIIDDGWFK
                     GRNDDRAALGDWYTDEQKYPNGLMPVIKHVKSLGMEFGIWVEPEMINPDSDLFRLHPD
                     WVLSMPGYSQPTGRYQYVLNLNIPEAFAYIYERFLWLLGEHPVDYVKWDMNRELVQAG
                     HEGRAAADAQTRQFYRLLDLLRERFPHVEFESCASGGGRIDFEVLKRTHRFWASDNND
                     ALERCTIQRGMSYFFPPEVMGAHIGHRRCHATFRQHSIAFRGLTALFGHMGLELDPVA
                     ADAKESDGYRRYALLYKEWRQLIHTGVLWRVDMPDPSIQVQGVVSPDQSQALFMISQL
                     AMPDYTLPGILRFPGLAAEVRYRLRVIDHPDLQVVGEGGHTMRKLPVWMNQSLEASGE
                     WLAQGGIQLPVLDPESAILIALERAV"
     gene            35713..36990
                     /locus_tag="OHN27_001588"
     CDS             35713..36990
                     /locus_tag="OHN27_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2286047.1"
                     /translation="MNSASTHKNPDFWIFGLFFFLYFFIMATCFPFLPVWLSDVVGLS
                     KTDTGIVFSCLSLFAISFQPLLGVISDRLGLKKNLIWSISLLLVFFAPFFLYVFAPLL
                     RFNIWAGALTGGVFIGFVFSAGAGAIEAYIERVSRSRGFEYGKARMFGCLGWALCAAM
                     AGMLFNVDPSLVFWMGSGSALLLLLLLFLARPSTSQTAMVMNTLGANSSLISTRMVFS
                     LFRMRQMWMFVLYTIGVACVYDVFDQQFATFFRSFFDTPQAGIKAFGFATTAGEICNA
                     IIMFCTPWIIHRIGAKNTLLVAGGIMTIRITGSAFATTATEVVILKMLHALEVPFLLV
                     GAFKYITAVFDTRLSATVYLIGFQFSKQLAAILLSTFAGHLYDRMGFQNTYFVLGMIA
                     LTVTVISVFTLSSSRGSVHPSVEKAPAAHSEIN"
     gene            36987..38420
                     /locus_tag="OHN27_001589"
     CDS             36987..38420
                     /locus_tag="OHN27_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCQ2286048.1"
                     /translation="MMKQRLALAQSALEKLCARRGNAWYPIFHLAPPAGWMNDPNGLI
                     YFNGRYHAFFQHHPASAYQGPMHWGHATSTDMLHWQHEPIALAPGDKYDRDGCFSGSA
                     VDDDGVLSLIYTGHICLDDRGNDSIIREVQCLATSHDGIHFEKQGCVLTPPEGIMHFR
                     DPKVWHEDGSWWMVIGARDASDNGQVLLYRGTSLRDWHLEHVLAHSAAGKSYMWECPD
                     FFRCGNFHWLMFSPQGMPPSGYRFRNLFQSGVLAGSWKPGSVFALKGGFEELDYGHDF
                     YAPQSMLAEDGRRIIMAWMNMWDSPVPTRSEAWAGCLTLPREVFERDGRLCQRPVREV
                     ESLRKKCQPLSPVRLQGLQLLTENVQAAELLVTWHTVDSHAEHYGVRLGDGLRLYVDN
                     QAGRLVLWRYYPEEGLDGYRSVELPDTEYLTLRIFLDRSSVEVFVNDGEATLSSRIYP
                     QADSRQLSLYAAHGDAILTDGTLWMLT"
     gene            38615..40009
                     /locus_tag="OHN27_001590"
     CDS             38615..40009
                     /locus_tag="OHN27_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCQ2286049.1"
                     /translation="MNKLLLGLSLFLLLEYNACAAKTGGLTLEQRMALLEERLEAAEK
                     RAEKAERMLKSFDIEQHSEIRQISSEQNKKDANSYAVVESTKEKTSSPGFLLSGYNDL
                     KFYGDVEFNIDAASKPGQLVMISSGANSESVNERWDLNGRILLGFDGTRKLDNGYFAG
                     FSAQPLADMHGSVNIDDALFFFGKDDEWKVKVGRFEAYDMFPLNQDTFIEYSGNTAND
                     IYADGRGYIYMMKEGRGRSDAGGNFLISKQLDNWYFELNTLLEDGTSLYNNGNYHGRY
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVKNAYGYTDNKGNFVDQSNRSGYGIS
                     MTWNGLKTDPENGIVVTLNTAYLDASNEKDFTASINALWKRFELGYIYAHNKIDEFSG
                     VVCNNGCWIDGEGIYNIHTIHASYQFANVMDMENFNIYLGAYYSILDSNCRYSNCDIT
                     DDRYGARLRFKYFF"
     gene            complement(40586..>40860)
                     /locus_tag="OHN27_001591"
     CDS             complement(40586..>40860)
                     /locus_tag="OHN27_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2286050.1"
                     /translation="ARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLM
                     AAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRASNGLSDNRCLEI"
BASE COUNT         9801 a        10390 c        10381 g        10288 t
ORIGIN      
        1 tataaatatt tgtgatctac aacacgcctt atctattgct tgtccgcaaa cggacatatc
       61 aaggtaataa aaaaggtcgc cgaagcgacc tttttacccg aaatgctaat tacagcagtt
      121 cttttgcttt cgcaacaacg ttatcaacag tgaagccgaa ctcttcaaac agcagctctg
      181 ccggagcaga ttcaccgaag gtggtcatac cgacgatagc accgttcagg ccaacatact
      241 tgtaccagta gtcagcaata cccgcttcta cagcaacgcg tgcagtaacc gctttcggca
      301 gtacggattc acggtaagca gcatcctgct tgtcaaatgc gtcggtagac ggcatggaca
      361 ccacgcgcgc tttcacgcct tcggcagtca gtttttcgta ggcagcaaca gccagttcaa
      421 cttctgaacc ggtagcgatg aaaatcagtt ccggctgacc ggcgcagtct ttcagcacat
      481 aaccaccgcg cgcgatgttt gccagttgct cttcagttcg ttcctgctgc gccaggttct
      541 gacgggagag gatcagtgcg gtcgggccgt cctgacgctc aacaccgtat ttccacgcga
      601 ccgcggattc aacctggtca cacggacgcc atgtagacat gttcggggtt acgcgcagag
      661 aagcgacctg ctcaaccggc tggtgagtcg ggccgtcttc gcccagaccg atggagtcgt
      721 gggtgtaaac catcacctga cgctgtttca tcagcgcagc catacgtacg gcgttacgtg
      781 cgtattccac gaacatcagg aaggtggagg tgtacggcag gaagccaccg tgcagggaga
      841 taccgttagc aatcgcggtc ataccgaact cgcgaacacc gtagtggatg tagttacccg
      901 cagcatcttc gttgattgct ttagaaccag accacagggt caggttagac ggcgccaggt
      961 cagcagaacc gccgaggaat tccggcaaca gcggaccgaa cgcttcgata gcattctgag
     1021 acgctttacg gctggcgatt ttcgccggat tagcctgcag tttagcgatg aactctttcg
     1081 ctttagcgtc gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat tcagcggctt
     1141 cctgcggata agctttcgcg taagcagcga atttctcgtt ccatgcggat tctttcgcct
     1201 ggcctgcttc tttcgcatcc cactgagcat agatttcaga cgggatttcg aacggcgcat
     1261 atttccagcc cagttgttcg cgggtcaggg caatttcagc gtcgcccagc ggcgcaccgt
     1321 gggagtcgtg ggtaccggct ttgttcgggg aaccgaaacc gatgatggtt ttgcacatca
     1381 gcagggaagg tttgtcagtc actgcgcgcg cttcttctac tgcgcgtttg atagatgccg
     1441 cgtcatgacc gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa cgcattgcgg
     1501 tgtcgtcggt gaaccagcct tcaacgtgac catcgataga aataccgttg tcatcgtaga
     1561 atgcaatcag tttacccagc ttcagcgtac ccgccagaga gcaaacttcg tgggagatgc
     1621 cttccatcat gcagccgtcg cccatgaagg cgtaggtgta gtggtcgaca atgtcgtggc
     1681 ccggacggtt aaactgcgcc gccagcgttt tttctgcaat cgccataccg actgcgttgg
     1741 caataccctg acccagcgga ccggtggtgg tttccacacc agcggtgtaa cccacttccg
     1801 ggtgacccgg agttttagag tgcagctgac ggaagttttt cagttcttcc atcggcagat
     1861 cgtaaccggt gaggtgcagc aggctgtaga tcagcatgga gccgtggccg ttggacagca
     1921 cgaagcggtc acggtcagcc caggacggat tctgcgggtt gtgtttcagg aaatcacgcc
     1981 acaggacttc ggcaatgtca gccataccca taggggcacc cgggtgaccg gatttggctt
     2041 tctgtactgc gtccatgctc agcgcacgaa tagcattggc aagctcttta cgtgaggaca
     2101 ttttgactcc agatcggatg atgaagggca cgcccttaac gacttgacga cagcgcgttt
     2161 tgggctacgc cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat gtacatggaa
     2221 catccttttg ccgcttcaga aatctctgga tcatgctcgc atgttgcgca atctactcgc
     2281 ccgtccgctg cgcttttcct tatactgaga ctgagcgtcg attcacctgc aaacggcgca
     2341 tttttagaat aatcctgacc ttgtgcggaa gagaaaacat gaaaattcgc gccttattgg
     2401 tagcaatgag cgtggcaacg gtactgaccg gttgccagaa tatggactcc aacggactgc
     2461 tctcatcagg agcggaagct tttcaggctt acagtttgag tgatgcgcag gtgaaagccc
     2521 tgagcgatca ggcatgtcag gagatggaca gcaaggcgac gattgcgcca gccaatagcg
     2581 aatacgctaa acgtctgaca actattgcca atgcgctagg caacaatatc aacggtcagc
     2641 cggtaaatta caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg gctaacggct
     2701 gtatccgtgt ctatagcggg ctgatggata tgatgacgga taacgaagtc gaagcggtga
     2761 tcggtcacga aatggggcac gtggcgttag gccatgtgaa aaaaggaatg caggtggcac
     2821 ttggtacaaa tgccgtgcga gtagctgcgg cctctgcggg cgggattgtc ggaagtttat
     2881 ctcaatcaca acttggtaat ctgggcgaga aattagtcaa ttcgcaattc tcccagcgcc
     2941 aggaagcaga agccgatgat tattcttacg atcttctgcg ccaacgcggc atcagcccgg
     3001 caggtcttgc caccagcttt gaaaaactgg caaaactgga agaaggtcgc caaagctcaa
     3061 tgtttgacga ccatcctgca tccgccgaac gcgcccagca tattcgcgat cgcatgagcg
     3121 cggatgggat taagtaaagc ctggtggtgt cgaacgtagg tcagataagg cgttcacgcc
     3181 gcatctgaca tgaatggcac atttgttacc ttgtgcgcat tgccggatgc gatgctggcg
     3241 catcttatcc ggcctacggg tgccgaacgt aggtcggata aggcgttcac gccgcatccg
     3301 acattaatgg cacgttttac ccgtgcgcat cgcatctggt gcttactcgc cctttttcgc
     3361 cgcctgaata tacagcattt ccagcgccag cgttgccgct gccagagcag tgatttccga
     3421 ctgatcgtat gccggagcca cttccactac gtccatccca acaatgttga gatctttcag
     3481 gccgcgtacc agtttaatag cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt
     3541 gcctggtgca aaagcaggat ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc
     3601 acccacaatc tgtttcactt gggcgataac gtcatccacg ctgcgatcgt tcacctggca
     3661 ggcgtccagc acggtaaagc cgttgtcttt atcaaactcg gtacgaatac caatctgcac
     3721 ggaatgattc gggtcgatca gaccttcttt cggcgcggta tagaacatag tgccgtggtc
     3781 aaattcacaa ccgttcgcat aggtatcggt gtgggcgtca aagtgtacca gcgccatttt
     3841 gccgaaatgc ttcgcatgag cacgcagcag cggcagcgta acaaagtggt caccaccgaa
     3901 agagagcata cgcttaccgg cagccagcag cttctcggcg tgcgcctgca gcttttcgct
     3961 catctcacgg gcatcgccaa aggcatatac cagatcgccg cagtccacga cgttcagacg
     4021 ctcacgcatg tcgaaattcc acgggaagcg gttgtgttcc caggccagat tcgtcgaaac
     4081 ctgacggatc gctgccggac cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa
     4141 cggcacgcca gtaatcaccc agtctgcatc gctgtcatac ggctggaagt tcatcggcag
     4201 gcgtaaaaaa ccaaaggcat tggaaaccag tgagttatcg tattgatgac ctaaggtgct
     4261 catggatgga ctcctattac aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg
     4321 acgaggaagg gttggatttg tcacaataaa ttgtggcgga ttatcaccgc taaattaagc
     4381 ggattcaagt aacacaggac ttactcatct tcaagataag tataaccgta caaacctgcc
     4441 tcgaactctt caaggaactg ttgttgcagt tcagcatcaa gatcggtttt cttcacttga
     4501 tcgcggaact gggttaacag cgttttcgga tcgagctgta catattgcag catgtccgcc
     4561 acggtatcgc cttcgtcaga cagttctact tctacgctac cgtcagggaa gacgaacacg
     4621 tcaaccgctt cggtatcacc gaacaggttg tgcatgttgc cgaggatctc ctgatatgcg
     4681 ccgaccataa agaaaccgag catcggcgga ttctctggat cgtactccgg cattggcatt
     4741 gtcgtggcaa taccgtcacc atcaatatag tggtcgatag caccgtcaga gtcacaggta
     4801 atatccagca gcacagcgcg acgttccggc acttgatcca gcccttccag cggcagaacc
     4861 gggaacaact ggtcgatccc ccatgcgtcc ggcatcgact ggaacagcga gaagttgacg
     4921 tacattttgt ccgccatacg ttcctgcagc tcgtcgataa tcggacgatg agcacggttt
     4981 tgcggatcca gctgcttttg cacttcatgg cacatgctca aataaagctg ctcagcccat
     5041 gcacgttctt gcaggctaaa gatgccggaa gagtagccga tatgaatgtc gtgcagatcc
     5101 atctgactgt cgtgtaacca ttcacgcaga gaacggcgag ttcccggttc gtgcatctcc
     5161 tgccaggttt cccacatgct ttgcagcgcg cgcggcgcat cttctgcagg cgcggtcggc
     5221 accgtgtatt cgttacgttc cacgccgatg atattagaca ccagcacggt gtgatgcgca
     5281 gtcaccgcac gacccgattc ggtgattacc gtcggatgcg gcagaccgtt ttcttcacac
     5341 gcatcgccaa tcgcccagat aatgttgttg gcgtattcat tgaggccgta gttcaccgaa
     5401 cagtcggact gcgaacgagt accttcataa tccacgccca gaccgccgcc gacatcgaag
     5461 cactgaatat tgacgcccag cttgtgcagt tccacataga aacgcgcgga ttcacgaacg
     5521 cctgtcgcga tatcgcgaat attcgccatc tgcgaaccga ggtggaagtg cagtagttgc
     5581 aggctgtcga gacgcccggc ttcacgcagg gtttcaacca gttgcagtac ctgagtcgca
     5641 gccaggccga acttcgattt ttccccgccg gaggactgcc atttacccga accctgcgaa
     5701 gccagacgtg cacgcacgcc cagacgagga acgacattca gacgttctgc ttcatccagc
     5761 acaatggcga tttctgacat cttctcaatg accagataga ccttgtgccc catcttctcg
     5821 ccaattaatg ccaggcggat atattcgcgg tctttataac cgttgcagac gatgacgcta
     5881 cgggtcatgc cagcatgtgc cagtactgcc atcaactcgg ctttggaacc ggcttccaga
     5941 cccagcggtt cgcccgaatg aatcagggac tcaatcacgc ggcggtgctg gttaactttg
     6001 atcggataaa caaggaagta atcgccgtta tagccgtagg attccctcgc acgtttgaac
     6061 gcggcgttaa tggaacgcaa acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc
     6121 agacgctggc cctgtgcttc acgagttttc actaactgcg cgagatcgac gcgagcttcc
     6181 gggacgtccg ggtccgggca cacgctaatg tggcccagct cgttaacgtc atagtagtta
     6241 ttgccccacc aggcaatatt gtaagtacgc agcatcttgc tggcttcctg ggagctcatt
     6301 gcaacctcct gcatggagcg tagtacaccg tgttcgcccg ctgacgaagg caaacccata
     6361 gacatgtcgt cagacatagc gaacctcaaa ttattttatt aagtgtaaaa cagttaacga
     6421 ctatcgcagc ctggaactgc gataacaacc cataagcaca tggattttcc agcagtgaat
     6481 gctgacgctc caactgcgca accggtttct ttttcataac attattaagc acataaccga
     6541 acgtaagtgt gaaagttcgg cgaaaccacg agaaaactct tgtttttaca agagcgccct
     6601 tgttcagtcc tcagtaactg taaccagctc ttgaatcctg agaagcgccg agatgggtat
     6661 aacatcggca ggtatgcaaa gcagagatgc agagtgcggg gaacgaatct tcaccagaac
     6721 ggtgagacag gttaagcagc agacaacggt tcattatttc gtatcacctc cacggccgcc
     6781 tgttaagacg aacccacaag ccaaaactct gatttcaacc cggctggaag tggcaacacg
     6841 aaagaaacgt cgtgtgcttt ttatttaagc cgcgcgccgc gttttatacc ccacaatggc
     6901 agaaaattgc aaaagataaa tacgcagaat gccggcattg tcaggaaaaa tttccagcca
     6961 cgtttttaac agaatgagac acgattcaaa aaaaagtgga aatagggtga agaattgacc
     7021 taaaatagcc atccagatgt taatccatcc ataccgatta acactcagac tgccagtgtt
     7081 tttaacctgc agagtcgtgg taggatccgc taccacagaa aatccacaca acagtttgag
     7141 ctaaccaaat tctctttagg tgatattaaa tatggcaaaa caccttttta cgtccgagtc
     7201 cgtctctgaa gggcatcctg acaaaattgc tgaccaaatt tctgatgccg ttttagacgc
     7261 gatcctcgaa caggatccga aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat
     7321 ggttttagtt ggcggcgaaa tcaccaccag cgcctgggta gacatcgaag agatcacccg
     7381 taacaccgtt cgcgaaattg gctatgtgca ttccgacatg ggctttgacg ctaactcctg
     7441 tgcggttctg agcgctatcg gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc
     7501 cgatccgctg gaacagggcg cgggtgacca gggtctgatg tttggctacg caactaatga
     7561 aaccgacgtg ctgatgccag cacctatcac ctatgcacac cgtctggtac agcgtcaggc
     7621 tgaagtgcgt aaaaacggca ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac
     7681 ttttcagtat gacgacggca aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca
     7741 ctctgaagag atcgaccaga aatcgctgca agaagcggta atggaagaga tcatcaagcc
     7801 aattctgccc gctgaatggc tgacttctgc caccaaattc ttcatcaacc cgaccggtcg
     7861 tttcgttatc ggtggcccaa tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga
     7921 tacctacggc ggcatggcgc gtcacggtgg cggtgcattc tctggtaaag atccatcaaa
     7981 agtggaccgt tccgcagcct acgcagcacg ttatgtcgcg aaaaacatcg ttgctgctgg
     8041 cctggccgat cgttgtgaaa ttcaggtttc ctacgcaatc ggcgtggctg aaccgacctc
     8101 catcatggta gaaactttcg gtactgagaa agtgccttct gaacaactga ccctgctggt
     8161 acgtgagttc ttcgacctgc gcccatacgg tctgattcag atgctggatc tgctgcaccc
     8221 gatctacaaa gaaaccgcag catacggtca ctttggtcgt gaacatttcc cgtgggaaaa
     8281 aaccgacaaa gcgcagctgc tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg
     8341 cgttcaaagg ccagcctcgc gctggccttt ttcttttgga taggcgttca cgccgcatcc
     8401 ggcaaaaaaa ccgcccgcac aataacatca ttcttcctga tcacgtttca ccgcagatta
     8461 tcatcacaac tgaaaccgat tacaccaacc acaacagaca aagatttgta atattttcat
     8521 attattattc ggttttcaca gttgttacat ttcttttcag taaagtctta attgcagata
     8581 acagcgttta atctatgatg atataactca attattttca tgcacttaaa tcataactaa
     8641 gataaatgtt agtgtaagcg attacactga tgtgatttgc ttcacatctt tttacgtcgt
     8701 actcacctat cttaattcac aataaaaaat aaccatattg gagggcatca tgcctgacgc
     8761 taaaaaacag gggcggtcaa acaaggcaat gacgtttttc gtctgcttcc ttgccgctct
     8821 ggcgggatta ctctttggcc tggatatcgg tgtaattgct ggcgcactgc cgtttattgc
     8881 agatgaattc cagattactt cgcacacgca agaatgggtc gtaagctcca tgatgttcgg
     8941 tgcggcagtc ggtgcggtgg gcagcggctg gctctccttt aaactcgggc gcaaaaagag
     9001 cctgatgatc ggcgcaattt tgtttgttgc cggttcgctg ttctctgcgg ctgcgccaaa
     9061 cgttgaagta ctgattcttt cccgcgttct gctggggctg gcggtgggtg tggcctctta
     9121 taccgcacca ctgtacctct ctgaaattgc gccggaaaaa attcgcggca gtatgatctc
     9181 tatgtatcag ttgatgatca ctatcgggat cctcggtgct tatctttctg ataccgcctt
     9241 cagctacacc ggtgcatggc gctggatgct gggtgtgatt atcatcccgg caattttgct
     9301 gctgattggt gtcttcttcc tgccagacag cccacgttgg tttgccgcca aacgccgttt
     9361 tgttgatgcc gaacgcgtgc tgctacgcct gcgtgacacc agcgcggaag cgaaacgcga
     9421 actggatgaa atccgtgaaa gtttgcaggt taaacagagt ggctgggcgc tgtttaaaga
     9481 gaatagcaac ttccgccgcg cggtgttcct tggcgtactg ttacaggtaa tgcagcaatt
     9541 caccgggatg aacgtcatca tgtattacgc gccgaaaatc ttcgaactgg cgggttatac
     9601 caacaccacc gagcaaatgt gggggacagt gattgtcggc ctgaccaacg tacttgccac
     9661 ctttatcgca atcggccttg ttgaccgctg gggacgtaaa ccaacgctaa cgctgggctt
     9721 cctggtgatg gctgctggta tgggcgtact cggtacaatg atgcatatcg gtatccactc
     9781 tccgtcggcg cagtatttcg ccatcgccat gctgctgatg tttattgtcg gttttgccat
     9841 gagtgccggt ccgctgattt gggtactgtg ctccgaaatt cagccgctga aaggccgcga
     9901 ttttggcatc acctgctcca ccgccaccaa ctggattgcc aacatgatcg ttggcgcaac
     9961 gttcctgacc atgctcaaca cgctgggtaa cgccaacacc ttctgggtgt acgcggctct
    10021 gaacgtactg tttatcctgc tgacattgtg gctggtaccg gaaaccaaac acgtttcgct
    10081 ggaacatatt gaacgtaatc tgatgaaagg tcgtaaactg cgcgaaatcg gcgctcacga
    10141 ttaatctcct caggcttcct cccgtcgcgg aggaagccgc ctcttgcagt catcttttct
    10201 tcgctctatc ctctgccgct atgaaaacat cccgtctccc tatcgccatc caacaggccg
    10261 ttatgcgtcg cctgcgggaa aaactcgccc aggctaacct gaaattgggg cgtaactacc
    10321 cggagccaaa actctcttac acccagcgcg gcacctccgc cggaacagcc tggctggaaa
    10381 gctatgaaat ccgcctcaat cccgttttgc tgttggaaaa cagtgaagcc tttattgaag
    10441 aagtggtgcc gcacgaactg gcacatttgc tggtatggaa acatttcggg cgcgtagcac
    10501 cacatggcaa agagtggaag tggatgatgg aaaacgtgct gggtgttccc gcccgtcgta
    10561 cgcatcagtt cgaactgcaa tccgtgcgtc gcaacacctt cccctaccgc tgcaagtgcc
    10621 aggagcatca gcttaccgta cgccgccata atcgcgtagt tcgtggcgaa gccgtctatc
    10681 gctgtgttca ctgcggcgaa caactggtcg caaaataacc atctgaacta tcaggaactt
    10741 tcctgatctg gctgattgca taccaaaaca actttcgcta cgttgctggc tcgttttaac
    10801 acggagtaag tgatgtaccg ttatttgtct attgctgcgg tggtactgag cgcagcattt
    10861 tccggcccgg cgttggccga aggtatcaat agtttttctc aggcgaaagc cgcggcggta
    10921 aaagtccacg ctgacgcgcc cggtacgttt tattgcggat gtaaaattaa ctggcagggc
    10981 aaaaaaggcg ttgttgatct gcaatcgtgc ggctatcagg tgcgcaaaaa tgaaaaccgc
    11041 gccagccgcg tagagtggga acacgtcgtt cccgcctggc aattcggtca ccagcgccag
    11101 tgctggcagg acggtggacg taaaaactgc gctaaagatc cggtctatcg caagatggaa
    11161 agcgatatgc ataacctgca gccgtcagtc ggtgaggtga atggcgatcg cggcaacttt
    11221 atgtacagcc agtggaatgg cggcgaaggc cagtacggtc aatgcgccat gaaggtcgat
    11281 ttcaaagaaa aagctgccga accaccagcg cgtgcacgcg gtgccattgc gcgcacctac
    11341 ttctatatgc gcgaccaata caacctgaca ctctctcgcc agcaaacgca gctgttcaac
    11401 gcatggaaca agatgtatcc ggttaccgac tgggagtgcg agcgcgatga acgcatcgcg
    11461 aaggtgcagg gcaatcataa cccgtatgtg caacgcgctt gccaggcgcg aaagagctaa
    11521 cctacactag cgggattctt tttgttaacc cctaccccac gcgtacaacc gcgtggggag
    11581 acgacgcgga tttttaacta tgcgtatccc ccgcatttat catcctgaac cactgaccag
    11641 ccattctcac atcgcgcttt gcgaagatgc cgccaaccat atcgggcgcg tactgcgcat
    11701 ggggccgggg caggcgttgc aattgtttga cggtagcaac caggtctttg acgccgaaat
    11761 taccagcgcc agcaaaaaaa gcgtggaagt gaaggtgctg gaaggccagc tcgacgatcg
    11821 cgaatctccg ctgcatattc acctcggtca ggtgatgtcg cgtggtgaaa aaatggaatt
    11881 tacgatccag aaatcgatcg aactcggtgt aagcctcatt acgccacttt tttctgagcg
    11941 ctgcggcgtt aaactggata gtgaacgtct gaacaagaag ctccagcagt ggcagaagat
    12001 tgcaattgct gcctgtgagc agtgtggtcg taaccgggtg ccggaaatcc gtccagcgat
    12061 ggatctggaa gcctggtgtg cagagcagga tgaaggactg aaactgaatc ttcacccgcg
    12121 cgccagtaac agcatcaata cgttgccgtt accggttgaa cgcgtccgcc tgctgattgg
    12181 cccggaaggc ggtttatcgg cagatgaaat tgccatgact gcccgctatc aatttactga
    12241 tatcctgttg ggacctcgcg ttttgcgtac agagacaact gcgctcaccg ccattaccgc
    12301 gctacaagta cgatttggcg atttgggcta acggagaaga ataatgatca agctcggcat
    12361 cgtgatggac cccatcgcaa acatcaacat caagaaagat tccagtttcg ctatgttgct
    12421 ggaagcacag cgtcgtggtt acgaacttca ctatatggag atgggcgatc tgtatctgat
    12481 caatggcgaa gcccgcgccc atacccgcac gctgaacgtg aagcagaact acgaagagtg
    12541 gttttcgttc gtcggtgaac aggatctgcc gctggccgat ctcgatgtga tcctgatgcg
    12601 taaagacccg ccgtttgata ccgagtttat ctacgcgacc tatattctgg aacgtgccga
    12661 agagaaaggg acgctgatcg ttaacaagcc gcagagcctg cgcgactgta acgagaaact
    12721 gtttaccgcc tggttctctg acttaacgcc agaaacgctg gttacgcgca ataaagcgca
    12781 gctaaaagcg ttctgggaga aacacagcga catcattctt aagccgctgg acggtatggg
    12841 cggcgcgtcg attttccgcg tgaaagaagg cgatccaaac ctcggcgtga ttgccgaaac
    12901 cctgactgag catggcactc gctactgcat ggcgcaaaat tacctgccag ccattaaaga
    12961 tggcgacaaa cgcgtgctgg tggtggatgg cgagccggta ccgtactgcc tggcgcgtat
    13021 tccgcagggg ggcgaaaccc gtggcaatct ggctgccggt ggtcgcggtg aacctcgtcc
    13081 gctgacggaa agtgactgga aaatcgcccg tcagatcggg ccgacgctga aagaaaaagg
    13141 gctgattttt gttggtctgg atatcatcgg cgaccgtctg actgaaatta acgtcaccag
    13201 cccaacctgt attcgtgaga ttgaagcaga gtttccggtg tcgatcaccg gaatgttaat
    13261 ggatgccatc gaagcacgtt tacagcagca gtaacccacc ttagcgagaa ggatctcgtt
    13321 gagactctga gtgacagcgc ccttctttcc acgcatactg ggcgctgttg cttttttgaa
    13381 ccaggaaaca gaacctctga caatgaattt acagcatcac tttcttattg ccatgcctgc
    13441 tctccaggat ccgattttcc gtcgttccgt ggtctacatt tgcgaacata ataccaatgg
    13501 tgcaatgggg atcatcgtca acaagccgct ggaaaatctc aaaattgaag ggattctgga
    13561 aaagctgaag atcacgccgg agccgcgtga tgaatcaatc cgtctggata aaccggttat
    13621 gctcggcggc ccactggctg aagatcgcgg gtttattttg catactccgc cctccaattt
    13681 tgcttccagc attcgcattt cagacaacac ggtgatgacc acttcccgcg atgtgctgga
    13741 aacgctcggc accgataaac aaccgtctga cgtattggtg gctctgggct atgcctcctg
    13801 ggagaaaggt caactggaac aagaaattct cgataacgcg tggctaaccg ccccggcaga
    13861 tctgaatatt ctgttcaaaa agccgattgc cgaccgctgg cgcgaggcgg caaaactgat
    13921 tggtgtggat attctcacca tgcctggtgt ggcagggcac gcctgatgag tggaacctta
    13981 ctcgccttcg acttcggcac caaaagcatt ggcgtagcgg tcggccaacg cattaccggc
    14041 accgctcgcc ctttgcctgc aattaaagca caggacggta cgccggactg gaacattatc
    14101 gagcgtttac tgaaagagtg gcagccggac gaaatcatcg tcggtttgcc gctgaatatg
    14161 gacggcaccg agcaaccatt gactgccaga gcgcgtaaat ttgccaaccg tattcatggc
    14221 cgtttcggtg ttgaagtaaa gctccatgac gagcgtctta gcactgtgga agcccgttcc
    14281 ggtctgtttg aacagggcgg ctatcgggcg ctcaacaaag gcaaagttga ctctgcctct
    14341 gcggttatta ttctcgaaag ctatttcgag cagggatatt aaggcgattt aaacgcctgg
    14401 cggagtgtaa ataatatcat cgcgtcttat tgccggatgc ggcgtgaaca ccttatccag
    14461 cacacatctg gcagcggcta taggtctgat aagacgcgtt agcgtcgcat cagacattta
    14521 ttgcctttgt aggcccgata agcttgcgca tcgggcatgg caacgtcaca aacgcccttc
    14581 ccccacccgg tgctgataac tctgctgaaa cgttatcatc cccacctgct gcccggtttg
    14641 aataacatgc ggtaactggt gggttttccc ttcgcgaatc aaattcccca ccgcgggtgt
    14701 gttaatcagc aattcaaata gcgccacgcg tccttcctgt ttatccactt ccagcttttg
    14761 tgacagcact gcccgtaaac tacctgccag ttgattacgc acggggtctt tttcctgcgc
    14821 cggaaatgaa tccaccagtc gctcaactgc ctgcgcggca ccacgcgtat gtaatgttgc
    14881 cagcaccaaa tgcccggttt ctgccgccgt cagtgccaga cggattgtct cgctgtcacg
    14941 cagctctccg agcaaaatca catcaggacc ttcccgcaat gcggcccgca atcccgatgc
    15001 gaacgtcata cagtgcaaac caatttcccg ctgctggatc aaacatcgct ggctggcata
    15061 gagatattca acaggatctt ccagcgtcag aatatgcgca tcggcatgtt gattgagata
    15121 gccaaccatc gccgccagcg tggtagattt gccactcccc gtcgcccccg tcaccagaat
    15181 caggccattc tcgctcttga gtaattccgg caataccgtt ggtgcgccaa gctgttcgag
    15241 ctgcgggcag tgcgaaggta acagccgtaa cgccagcgaa atgccatgcc gttgcgcgaa
    15301 tgcgctgcca cgcaatcgct ggttttccgc cagcgacacg gcaaaatcca gctgaccatt
    15361 ctccagcaat attgcccgct gatcgtcatc cagccactcc cgcagtagct cttcgacgtc
    15421 cggcgtatca aacggcgcag cttccattct gccgcgaatg cgccatcgtg cgggccaggc
    15481 gctgcacagg tgtagatccg agacgttatg ctttacacta agggccacaa tttcttccat
    15541 attcatacta agatcctcgg aaaatgaacg atattgcgca taacctggca caggtccggg
    15601 acaaaatctc agcggctgca acgcgttgcg gccgttctcc agaagaaatt acgctgcttg
    15661 cagtcagtaa aacaaaacct gcgagcgcca tcgcagaagc cattgatgcc gggcagcgtc
    15721 aatttggtga aaactacgtt caggaagggg tagataaaat tcgccacttt caggaactgg
    15781 gcgtaacagg attagaatgg cattttattg gcccgttgca gtctaataaa agccgcctgg
    15841 tggcagagca tttcgactgg tgtcatacca tcgaccgttt gcgcatcgct acccgtctca
    15901 acgaccagcg cccggcagaa cttccccctc ttaacgttct gattcaaatt aacattagtg
    15961 atgaaaacag taagtccggg attcaactgg ctgaactgga cgagctggca gctgcggtcg
    16021 ctgaactacc gcgtttacgt ctgcgcgggt tgatggcaat ccctgcgcct gagtcagaat
    16081 atgtaaggca gtttgaagtt gcacgccaaa tggctgtagc atttgccgga ctgaaaacgc
    16141 gctacccgca tatcgacacg ctctctctgg gaatgtcgga cgatatggaa gccgccattg
    16201 cggcaggtag cacgatggtt cgtatcggca ctgcaatttt tggtgcgcgt gattactcta
    16261 aaaaataagg aattaaagga acgccatgaa tacgttgact ttcctgcttt caacggtcat
    16321 tgagctgtat accatggtgc tgttattacg catctggatg cagtgggctc attgtgattt
    16381 ttacaacccc ttctcacagt ttgtagtgaa ggtaacgcag ccaattatcg ggccactgcg
    16441 ccgcgttatt ccggcaatgg ggccaattga cagcgcctcg ctgctggttg cctatattct
    16501 cagttttatc aaagccattg tgctgtttaa agtggtgacc ttcctgccaa tcatctggat
    16561 tgccggttta ctgattctgc tgaaaaccat cggcttgctg attttctggg tcctgctggt
    16621 gatggcgatt atgagctggg taagccaggg tcgtagtccg attgaatacg tgctgattca
    16681 gctggccgat ccgctgctgc gcccgattcg ccgcctgcta ccggcaatgg gtgggattga
    16741 tttctcgccg atgatcctcg ttctgctgct gtatgtcatc aatatgggtg tcgcagaagt
    16801 attacaggca accggaaaca tgctgctgcc ggggctgtgg atggcgttat gagtgccgta
    16861 acagttaatg atgacggtct ggttttacgg ctctatattc agccgaaagc cagccgtgat
    16921 tctattgtcg gtttacatgg cgacgaagtt aaagtcgcca ttaccgcgcc gccggttgac
    16981 ggccaggcca acagccatct ggtgaagttt ctcggtaagc aattccgggt agccaaaagc
    17041 caggtggtaa ttgaaaaagg cgaacttggc cgccacaaac aaattaaaat cattaatccg
    17101 caacaaatcc cgccagaaat cgcggcgtta attaattagg tatcctatgc aaaaagttgt
    17161 cctcgcaacc ggcaatgccg gtaaagtgcg tgagctggcg tcgctgctta gcgacttcgg
    17221 tcttgatatc gtggcccaaa cagacctcgg cgtagattcc gctgaagaaa ccggcctgac
    17281 ctttatcgaa aacgcgattc tgaaagcgcg ccatgcggca aaagtgaccg gtttaccggc
    17341 aattgccgac gactctggtc tggcggtaga tgcgctgggt ggcgcgccgg gtatttactc
    17401 cgcgcgttat tccggtgaag acgcgaccga tcaaaagaat ctgcaaaaac tgttggaaac
    17461 actgaaagac gtaccggacg accaacgtca ggcgcgtttc cactgcgtgc tggtatatct
    17521 gcgtcacgcg gaagatccga ctccgctggt gtgccacggt agctggccgg gcgtgattac
    17581 tcgtgaacca gcgggcactg gtggctttgg ttatgatcca atcttcttcg taccttccga
    17641 agggaaaacc gctgccgaac tgacccgcga agaaaagagc gccatttccc accgtggtca
    17701 ggcattgaaa ctgctgttgg acgctttacg taatggttaa attaccgccg ctgagtctct
    17761 acattcacat cccgtggtgc gtgcagaaat gcccgtactg cgatttcaac tctcacgcgt
    17821 tgaaaggaga agtgccgcac gacgattacg ttcagcatct gctttgcgat ctggacaatg
    17881 atgtggctta cgctcagggc cgtgaagtaa agacaatctt tattggcggt ggtacgccga
    17941 gcctgctttc cggcccggcg atgcaaacgc tgctggacgg cgtgcgtgcg cgtttgccgc
    18001 tggcagcgga tgcagaaatt actatggaag cgaaccctgg tacggtagaa gccgatcgct
    18061 ttgtcgatta tcagcgtgct ggtgtgaacc gcatctctat tggtgtgcag agttttagcg
    18121 aagaaaagct gaaacgactt gggcgcattc atggcccgca agaagcgaaa cgagcggcaa
    18181 agctggcgag cggtttaggg ttacgtagct ttaaccttga tttgatgcat ggactaccgg
    18241 atcaatcact ggaagaggcg cttggcgatc tacgccaggc cattgaactg aatccgccgc
    18301 atctttcctg gtatcaactg accatcgaac ccaatacgct gtttggttcg cgaccaccgg
    18361 tgctgccgga cgacgacgcg ctgtgggata tattcgaaca ggggcatcag ttattaaccg
    18421 cagcgggtta tcagcaatat gaaacttccg cttacgccaa acccagttat cagtgccagc
    18481 acaatctcaa ctactggcgc tttggcgact acatcggtat tggctgcggc gcgcacggca
    18541 aagtgacctt cccggatggg cgcattctgc gtaccactaa aacgcgtcat ccgcgtggtt
    18601 ttatgcaagg aaggtatctg gaaagccagc gtgatgtcga agccgcagat aagccgtttg
    18661 agttctttat gaaccgcttt cgcttgctgg aggccgcgcc acgcgcagag tttagtgcgt
    18721 ataccgggct ttgcgaagat gtgattcgcc cacagttaga cgaggcgatt gcccagggtt
    18781 atctcaccga atgtgcggat tactggcaga taacggaaca tgggaagctg tttttaaatt
    18841 cgctgctgga gctttttctg gctgagtaaa cttgtattgc cggatgcggc gtgaacgcct
    18901 tatccagccg acatgtggca gcggttgtag gtctgataag acgcgcaagc gtcgcatcag
    18961 acgttgattg ccggatgcgg ggtcaacgcc gcatcctgct acaaatcgtg cactatatca
    19021 aacttactta atcaccccat tgcgccgcag ttgttcggta tcttttttcc agcgttcatt
    19081 agcttggttg atatcgtcaa ttttcatcaa aatcgcgact tttttactgt ttatttctga
    19141 aactttcatc gttaacggat attgcccttt ttcgagcgtt acccagtcat cctgcaattt
    19201 acggctgagt ttttgtgatt gcagaaaagt ctgtcgccgt tgttcgttga tatcgaaatc
    19261 tcgtttctgc tgaatcggca cttcataaca ctcttcactc tgtttatgac atgccgcaat
    19321 cgccggttga taaacctctt tgtcatattt aaccgcgaac gcctcgctat cgttctgttt
    19381 attaaactca tcacgctgtt ggtgaatttt tttaaaggct tcttcgcggg tcatttgctt
    19441 accgtttgca tcttttcccc aatatgcatc cgctgctttc tgcgccgcca ccatttgctt
    19501 atcaagcgct ttcacttcct tttccagcgc aggaatcgag ttcttctggg tattcaacgc
    19561 agattcaagc tgactaaagc gataattgaa atcttcgcta ttcacgatca gatatttatt
    19621 attcgttttg atatcgtcga ccacccgccc acgatcgctg gctgccgcct gaaaagagta
    19681 aaagttcacc gaccagccag acgcgggcgt tcctttactg gttaacatcg ccgagaattt
    19741 taccggttta tctttcgtcc aggtctgttc gagcagttcg taatctgcca actgaccgac
    19801 ccaggtatag agatcgtcac tggaagagac atcgccttcc gctgaccagg tggcctgatt
    19861 acccttagca tcaagatttt ttaaggtgat agcatcaagc ttgagaatgc cgtgatactg
    19921 ctttttgaat tgatctttta agatattttc agttggcggt tcgccatctg cccaggcatt
    19981 accagtcatc aacataagca gtgccgtgcc aacaatccat tgctttttca cccgaaaaac
    20041 ctcacgcgga ataatttatt ccggtgatta tcattagggt aatgaaaatt ggcaatggac
    20101 gtaagtctga agtgaaaaag ccccggcacg ataccggggc gaggtgatta gtactgattg
    20161 aagatctgct ggatctgctg cggatctttg gtttgcgtca gagccagctg cagcaggacg
    20221 cgcgcttttt gcgggttcag cgtgccagag gcgacgaagc cgtatttcgc atcatccact
    20281 tcagcatcct gagtggtagc acccgtcggt acgcgggaag aacgcactac tgcagtgccg
    20341 tttttcgcgg cggttgccag ggtgtcgaac acggatttat acaggttacc attacccaca
    20401 ccagcgctaa cgatgccatc atagcccgca tctaccagtg ctttagccgg aagatcggat
    20461 gcgttagcgt agttataaac gatgccgact ttcggcagct cattcagctt agagacatcg
    20521 aatggcgtat cgctggtgtg cttacgtgcc ggggtacgtt ggtagtcaat cttaccgttg
    20581 tgaatgtatc ccagaggacc gtagttaaca gacttgaagg tcgctacgtc ggtggtgttg
    20641 gttttggtga catcgcgacc gtccagtacg gtgtcgttca tcaccaccag cacgccacga
    20701 ttagcggagg ctttatcagc tgcggtcact accgcgttat acaggttgaa tggaccgtct
    20761 gcgctcatgg acgtggacgg gcgcattgcg ccgaccatca ccaccggttt gtcgcatttc
    20821 accgtcaggt cgaggaagta agcggtttct tccatcgtgt cggtaccgtg ggtaatgacg
    20881 aagccgtcgg ttttatcgca gtcggtgtta atttttttcg ccagtgtcag ccagacatta
    20941 tcgttcatgt cctgggagcc gatattcact acctgctcgc ctttaacgtt cgcaatgtct
    21001 tttagttgcg gcaccgcatt aaccagattt tctacgccaa ctttacccgc tgtgtagtta
    21061 gatttggttg cggagtcacc accaccggca atggtcccgc cggttgctaa aatggtgata
    21121 ttgggtaatg ccaatgctgc accactaaaa cccataacca gtgcggcaag tgccgtcttt
    21181 ttgaaaaact ccatttcatt cctccagtta cgtgaacgct acgcattatc ccttagctct
    21241 gtatgggaaa tttgacgtta aacaatttac aacgtgaata tattttggag atctacaaag
    21301 ttagaggcag gtaacaaaac gaagaattaa acggcataaa aaagtattat gccgtcttaa
    21361 aatagaggat tattttaaat tcccgaccag ggctttgcgg ctatcttcca gagtcacaac
    21421 gcggctacaa acatctttgc caaactgctg gaaatctttt tcctgctttt tccactcggt
    21481 ttggattgag gattgcagcc cccccaggct tcccagcaca ttctgtaatg ggttaccgcc
    21541 gcttttcagc accgctttcg cgcccatttc attaatgctg tcctgtaaaa ttccacccat
    21601 tgcctgattc actaattgct ggccttcggc gcgaacctga tcaatggctt tatagtgaaa
    21661 cgtcaggcca tcgctgcgcg tttcgataat gcggttcatc tgctctttca gctgcgcatc
    21721 aagtttggtc agacggctgc gcattttgct gctttcgccc atctcctgaa cgataatttt
    21781 atccagcgca atacgggctt tttcgacgcg gcttttcgcg ccttcatcaa tccacggcag
    21841 cgtgctgcgt agttcagcct gataatcctt cgcctgctcg cgctgggcgg cattcaggga
    21901 atattgctta ccgttataca tcacgttgcc gtctggcgtg atcaccagat tgccgttttc
    21961 gcccttcacc tgcacggttt gcgggctgac aatcacatcg tcacgcggcg tgacgctgca
    22021 ctggtagtcg gcgtgagcgg tcattgccgt cactgaaagt gctgccgcca gcagcatttt
    22081 gcgcatcata gtcttccctc aagaaaaaat caggccagca tttgctggcc ccagattgat
    22141 aacaaagtac gcgttttcca tgccggatgc ggcgtaaaca ccttatcccg cctactcgat
    22201 cgtgcatctt tccaggcctg ataagcgtag cgcatcaggc agttttgcat ttgtcatcgc
    22261 ccgtatgctt tcttagtccc accaaacgtc gaaaagttcg ctggtgcgta cctcatccag
    22321 tttgcgctct tccagccact tacgcacaat cgcctgatgt tcttcggtgc atttgccgat
    22381 ttcctgcatg cagatcagac cttcccaggc cagataaccg ctgccgtcaa aggccagttt
    22441 gttcggttcg ataacctcgt taataaaatc atcaacggtt ttatcaatct gttcttccga
    22501 tgtaccttcc gggaatcgcc atgccaccga aaatcctaat tcctggaatt cgtcgatgtg
    22561 cattttttta cgcagacgac ggctacggtt ctttgccatt atttcaccct ctcgaacatt
    22621 aagtcccata ctccgtgacc aagacgatga ccacgttgtt caaatttcgt caccggacgt
    22681 gatgccggac gcggtacgta atcattgctc tctgacaggt ttttataacc gtcaatagaa
    22741 gacatcactt caagcatatg ttccgcataa ggttcccagt cggtcgccat atggaatacc
    22801 ccccccagct gcagtttgct ttttaccagt tcggcaaacg gcacctgtac gatacggcgt
    22861 ttattatggc gcgctttgtg ccacgggtca gggaaaaaga gctgcaccat gcgcaatgaa
    22921 ttgtcaggaa tcattttatg cagcacttca accgcatcgt gacacatcac tcgcaggttg
    22981 cttaaacctt cttcatgcgc agaagccagg cacgcaccaa cgcccggtga atgcacttca
    23041 atgccgagga agtcctgctc agggcgatct ttagccattg ccaccagcga cgcccccatg
    23101 ccaaaaccaa tctcaagcgt caccggcgct tcacggccaa aaagcgcggg gaaatccagc
    23161 atatcttcgc tgaactcaac gcccatcacc ggccagtagt tttccagcgc atgttcctgg
    23221 cctttggtca gtcgcccctg gcggcgcaca aaactacgga tacggcgcag tgggcggccg
    23281 ttttcatcaa attccggtga aatgacgtcg tttttcataa agatttagtc gcttgtgaaa
    23341 gtgttctgaa aacgggcatt atccaaagtt agttgccgga tgcaagcatg ataaggccgt
    23401 ggctgcggaa agttccggtt tacaccctgc cgtcgctgtg ctgcaatctt gcccccaaca
    23461 acagtgaatt cggtgaccat gcaagcgtcg caattttcag cccaggttct ggactggtac
    23521 gataaatacg ggcgaaaaac gctgccctgg caaattgaca agacgcccta caaagtatgg
    23581 ctctcagaag tgatgttgca acaaactcag gttgcgaccg ttatccccta ttttgaacgc
    23641 tttatggcgc gcttcccgac ggtgaccgat ctcgccaatg cgccgctcga cgaagttctc
    23701 cacttgtgga ccgggcttgg ctattacgcc cgcgcgcgca atctgcataa agcggcacaa
    23761 caagtggcga ccttacacgg cggtaaattc ccggaaacct ttgaagaagt cgcggcgtta
    23821 ccgggcgtcg ggcgttccac cgcaggcgcg attctctcgc tttctctggg taagcacttt
    23881 ccgattctcg acggtaacgt caaacgcgtg ctggcgcgct gctatgctgt aagcggctgg
    23941 cctgggaaaa aagaggtcga gaataaatta tggagtttga gcgagcaggt gacgcccgcg
    24001 gttggcgtgg aacggtttaa tcaggcgatg atggatttgg gtgcgatgat ttgtacgcgc
    24061 tcgaaaccga aatgttcgct ctgtccgcta caaaacggat gtattgccgc cgccaacaat
    24121 agctgggcgc tttatccggg caaaaaaccg aaacagacgc tgccggagcg caccggctac
    24181 tttttgctat tacagcacga agatgaagta ttgctggcgc agcgtccgcc gagcggattg
    24241 tggggcggtt tatactgttt cccgcagttt gccgacgaag aaagtttgcg gcagtggctg
    24301 gcgcaacggc agattgctgc cgataacctg acgcaactga ccgcgtttcg gcataccttc
    24361 agccatttcc acttagatat tgtgcctatg tggcttcccg tgtcgtcatt caccggctgc
    24421 atggatgaag gcaatgcgct ctggtataac ttagcgcaac cgccgtcagt tggcctggcg
    24481 gctcccgtgg agcgtttgtt acagcagtta cgcactggcg cgccggttta gcgcgtgagt
    24541 cgataaagag gatgatttat gagcagaacg attttttgta ctttcctgca acgtgaagca
    24601 gaaggtcagg attttcagct gtaccccggc gagctgggaa aacgcatcta taacgagatc
    24661 tccaaagaag cctgggcgca gtggcagcac aagcaaacca tgctgattaa tgaaaagaaa
    24721 ctcaacatga tgaatgccga gcaccgcaag ctgcttgagc aggagatggt caacttcctg
    24781 ttcgagggta aagaggtgca tatcgagggc tatacgccgg aagataaaaa ataaaaacag
    24841 tgccggagca cgcctccggc aacttgcata aaaacaaaca caacacgcac ccggaatgat
    24901 gaaaaaatat ctcgcgctgg ctttgattgc gccgttgctc atctcctgtt cgacgaccaa
    24961 aaaaggcgat acctataacg aagcctgggt caaagatacc aacggttttg atattctgat
    25021 ggggcaattt gcccacaata ttgagaacat ctggggattc aaagaggtgg tgatcgccgg
    25081 tcctaaggac tacgtgaaat acaccgatca atatcagacc cgcagccaca tcaacttcga
    25141 tgacggtacg attactatcg aaactatcgc cgggacagaa cctgccgcgc atttgcgccg
    25201 ggcaattatc aaaacgttgc tgatgggtga cgatccgagt tcggtcgatc tctattccga
    25261 cgttgatgat attacgattt cgaaagaacc tttcctttac ggtcaggtgg tggacaacac
    25321 cgggcagccg attcgctggg aaggtcgcgc aagcaacttc gcggattatc tgctgaaaaa
    25381 ccgtctgcaa agccgcagta acgggctgcg tatcatctac agcgtcacca ttaacatggt
    25441 accgaaccac cttgataaac gtgcgcacaa atatctcggc atggtccgcc aggcgtcacg
    25501 gaaatatggc gttgatgagt cgctgattct ggcaattatg cagaccgaat cttcctttaa
    25561 cccgtatgcg gtcagccgtt ccgatgcgct gggattaatg caggtggtac aacatactgc
    25621 cgggaaagat gtgttccgct cgcaggggaa atccggcacg ccgagccgca gtttcttgtt
    25681 tgatcctgcc agcaatattg ataccggcac cgcgtatctg gcgatgctga acaatgttta
    25741 tctcggcgga attgataacc caacatcgcg gcgttatgcc gtcatcaccg cctataacgg
    25801 cggcgcaggc agcgtgctgc gagtcttttc gaatgataag attcaggctg ccaatattat
    25861 taacaccatg acgccgggcg atgtttatca gacgctgacg acccgccatc cctctgcgga
    25921 atctcgccgt tatctttata aagtgaatac cgcgcaaaaa tcctaccgcc gccgataatt
    25981 ccattaaccg cccctgacga tgctcagggg caaaaatgtt atccacatca caatttcgtt
    26041 ttgcaaattg ggaatgtttg caattatttg ccacaggtaa caaaaaacca gtccgcgaag
    26101 ttgatagaat cccatcatct cgcacggtca aatgtgcttt ttcaaacact catccgcatc
    26161 acgatgtgag gaaattaaca tgaatcttaa gctgcagctg aaaatcctct cttttctgca
    26221 gttctgtctg tggggaagtt ggctgacgac cctcggctcc tatatgtttg ttaccctgaa
    26281 gtttgacggt gcttctattg gcgcagttta tagctcactg ggtatcgcag cggtctttat
    26341 gcctgcgctg ctggggattg tggccgacaa atggttaagt gcgaaatggg tatatgccat
    26401 ttgccacacc attggcgcta tcacgctgtt catggcggca caggtcacga caccggaagc
    26461 gatgttcctt gtgatattga ttaactcgtt tgcttatatg ccaacgcttg ggttaatcaa
    26521 caccatctct tactatcgcc tgcaaaatgc cgggatggat atcgttactg acttcccgcc
    26581 aatccgtatc tggggcacca tcggctttat catggcaatg tgggtggtga gcctgtctgg
    26641 cttcgaatta agccacatgc agctgtatat tggcgcagca ctttccgcca ttctggttct
    26701 gtttaccctg actctgccgc atattccggt tgctaaacag caagcgaatc agagctggac
    26761 aaccctgctg ggcctcgatg cattcgcgct gtttaaaaac aagcgtatgg caatcttctt
    26821 tatcttctca atgctgctgg gcgcggaact gcagattacc aacatgttcg gtaatacctt
    26881 cctgcacagc ttcgacaaag atccgatgtt tgccagcagc tttattgtgc agcatgcgtc
    26941 aatcatcatg tcgatttcgc agatctctga aaccctgttc attctgacca tcccgttctt
    27001 cttaagccgc tacggtatta agaacgtaat gatgatcagt attgtggcgt ggatcctgcg
    27061 ttttgcgctg tttgcttacg gcgacccgac tccgttcggt actgtactgc tggtactgtc
    27121 gatgatcgtt tacggttgcg cattcgactt cttcaacatc tctggttcgg tgtttgtcga
    27181 aaaagaagtt agcccggcaa ttcgcgccag tgcacaaggg atgttcctga tgatgactaa
    27241 cggcttcggc tgtatcctcg gcggcatcgt gagcggtaaa gttgttgaga tgtacaccca
    27301 aaacggcatt accgactggc agaccgtatg gttgattttc gctggttact ccgtggttct
    27361 ggccttcgcg ttcatggcga tgttcaaata taaacacgtt cgtgtcccga caggcacaca
    27421 gacggttagc cactaattac gcaaagaaaa acgggtcgcc agaaggtgac ccgttttttt
    27481 tattcttact tcaacacata accgtacaac cgtttcacgc catccgcatc ggtttcgcta
    27541 taaacacctt gcagctccgg cgaaaatccc ggcaacaaat tcaccccttc ttccagtgca
    27601 aggaaataac gttgaaccgc cccaccccag acttccccgg gtaccacgca aagcacgcca
    27661 ggtggataag gcaacgcccc ttctgccgca attcgccctt cggcatcacg aatccgcacc
    27721 aactccacgt caccgcgaat ataagcgcta tgcgcatcct gggggttcat caccactgac
    27781 gggaaactct gctggcggaa catcgctttt tgtaggtctt tgacgtcgaa actgacatac
    27841 agatcgtgca tctcctgaca caactggcgc agggtgtagt cgcgatagcg caccggatac
    27901 ttgttataaa cgctcggcaa cacctcaacc agcggcgagt catcctcaat atgctgttca
    27961 aattgcgcca gcatcgccac cagttgtgcc agcttctcgt ggctttccgc cggagttaat
    28021 aaaaacagaa tggagttgag atcgcacttc tccggcacaa tgccgttctc acgcagatag
    28081 tgcgccagaa tcgtcgccgg aacgccaaag tcgctatatt cgccggtttc ggcatcgata
    28141 cctggtgtag tgagtaacag cttgcacgga tcaacaaaat actgatccgc ggcatatcct
    28201 tcaaagccgt gccacttcgc ccccggctca aaactgaaaa aacggcggtc gctggctaac
    28261 actgatgtcg gataatcctg ccacaatttg ccatcaacaa cgggcgggat aaacgggcgg
    28321 aacagcttac agcgcgcaag aatagccttg cgcgcttcaa tccctatctc aacacactca
    28381 gcccacagcc gacgcccact ctccccttca tgaattttgg cgttaacatc cagtgcagca
    28441 aacagcggat agaaagggct ggtagaagca tggagcataa aggcgttatt caaccgctta
    28501 tgcgggcaaa aacgcgcctg tccgcggata tggttatctt ttttatggat ctgcgacgtc
    28561 tgtgagaatc ccgcctgctg tttgtgcacc gactgagtca caaagatccc cggatcgttt
    28621 tcgttaagtt ctaacagcag cggcgagcta tccgccatca tcgggataaa ttgttcataa
    28681 ccgacccacg cggaatcaaa cagaatgtaa tcacacagat gcccaacggt atcgatcacc
    28741 tgacgggcgt tatagacagt gccgtcatag gttcccagct gaataatcgc caggcgatac
    28801 gggcgcggca ggtcggcttt ttctggcgca acgtcgcgaa tttgctggcg cagatactct
    28861 tcattaaaac agtgcgcatc aataccgcca atgaaaccaa acgggttgcg tgaagcttcc
    28921 agatagaccg gcgtcgcccc cgcctgaatc agcgcgccgt gatgattcga cttatggttg
    28981 ttacggtcga agagcaccag atcgccacgc gttaacagcg cattcgtcac cactttattc
    29041 gctgccgatg tgccgttcag cacaaaatag gttttatcgg catgaaagac tttggctgcg
    29101 aatttctgcg catctttcgc cgatccttca tgaataagca gatcgcccaa ttttacgtca
    29161 gcgttacaca tatcggcgcg aaagacgttc tcaccaaaga aatcgtaaaa atggcgtccg
    29221 gcaggatgct ttttaaaaaa cgcaccatgt tgatgtccag ggcaagcaaa ggtgctgttg
    29281 cccatctcaa cgtactgcgt cagcgtgtca taaaacggtg gcagcaaatt ctcttcatac
    29341 tgacaggctg cggattccag ctccagccac tgctgctcgt tgccgttgat taccgccgta
    29401 acgcccgcag gtaattcaac agcatgttcg gaatacaaaa acaccggtag atgaaaaccg
    29461 gtgcgcttaa gcaacgcaag aatgccactg cgactatccg cagcggtaat gacgactgcc
    29521 gcgacgtccg taaaatcagt atctcccaac gccaccacgc gacgatgaga agaaagtcgg
    29581 gataccagtt cactactggc ggcaatattc attgatttca taagcgcaaa cccgtttcgg
    29641 ggaagtaaga ataccggaca aggtggaaaa ccctgcccca tgagatatgg gtcaaactgg
    29701 tcaccagctc cgaccgccag acatcagtaa aagcagaaac gctctgattt tactgttgtc
    29761 ctgcagtgag cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg gaaacgtttc
    29821 gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg caaactccgt
    29881 acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaagcgtgc aagccaggac
    29941 tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa tcaaacaata
    30001 cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc agtcttactg
    30061 ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg cgtctccatg
    30121 acatcaattt ttggtctggc atcgctgggg attggtattt tactggtggt gaaatgtgcc
    30181 aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga aatttgtttg
    30241 ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg tcactcacgt
    30301 aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt cctgttttgc
    30361 gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga tccgagtatt
    30421 ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg ctcgctgggt
    30481 attgcggtgt cggtaattag tatcccatta ctgatcatcc agttaacgct ggcgtgggct
    30541 gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag cgccgtaggc
    30601 ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt cccggtggtc
    30661 aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac cgcctggttt
    30721 gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct gcagtaaatt
    30781 gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt tgcccggata
    30841 gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat tccgagtccg
    30901 ggcaccactt tcacttccag attcatctcc cgaactccac gaaactcaag tataatcaca
    30961 tcgttacaag caatctgaac attctgaact ccaccagact ctactgaact ctctctcaat
    31021 caagcgttca ttgggggccc cggtgggggc tcattgtgtt cagtcagttt caggagcccc
    31081 catatggcac ttacagccag acaggtcgaa acagccagac ctaaagaaaa agactataaa
    31141 ctctctgacg aacgtggttt atatctgctg gtaaaaacca cgggtgcccg ctactggcgg
    31201 cttaaatacc ggatagcagg aaaagagaaa aaactggccc tcggcgtcta tcccgacgtc
    31261 tcccttgctg aggccagaat caaacgcgac gatgcccgaa aaatcatctc cgaaggtggt
    31321 gacccgggcg aaaagaagcg aaaggaaaaa ctcactcaga aaatctctgc caccaatacg
    31381 ttccatgccc tcgctacgga atggcaccag cataaatctt tgtcatggtc tgaaagttac
    31441 gccagaagcg tactggaagc gctggataaa gatattttcc cgtatctggg caaacgaagc
    31501 gttacggata tcctcccgct ggaaatgctg gaaattctgc gccgcataga aaaacgtggc
    31561 tcgctggaaa aacttcgtaa ggtgcgtcaa tactgtaatc agatttttcg ttatgccatc
    31621 gccaccggac gagccactgt caatccggca tctgaactga ccagtacgct ggcggcgcca
    31681 aaagctgcac atttccccca cctgagagca gatgagctcc ctgtttttct ccggaagctc
    31741 gctgagtatc atggcagtcc tgttacccgc atggcgacaa atctgctgct tctgacaggt
    31801 ctcagaacca ttgagctacg gtccgctgaa tggtcagaaa ttgattttga taatgcactg
    31861 tggacaatcc ctgaaagccg catgaaaatg cgacgtaaac atgtcgtacc actgtcacga
    31921 caggccactg acattctgct gcagctcaaa actttctccg ggcaataccg gctggttttt
    31981 ccgggacgtt gtgatatcaa caagccaatg agcgaagcca gcatcaatat ggtgctcaaa
    32041 cgtatcggtt acgatggcag ggcaaccggt catggttttc gtcacaccat gagtaccatt
    32101 ctgcacgaac agggctttaa ttctgcctgg attgaaatgc agttagctca tgtggataaa
    32161 aattccatca ggggtaccta taatcatgcc ctgtatctcg atggtcgccg tgaaatgatg
    32221 caatggtacg ctgactacat tgattcactt tccatccagg agagttaatt acactttgtc
    32281 ctgaaattat tctcacaatc aataaccttt ccctctgatc tgtaagatca gagggattcc
    32341 gttaacttac aataagtgat attggttgtc tggagattta gggggacggt ctactataat
    32401 aattagcagg agataatatg tcacttaagg cgattgccac tgcactcgga gtttctgtca
    32461 ccactgtcag tcgggctctt ggaggctatg cagatgtgtc agcttccacc cgtgcacggg
    32521 tggaagctga agctcgtcga cgcggttacc gtcctaatac tcaggcaaga agactcaaaa
    32581 ccggtaaaac cgatgctgtc ggtctggttt atcctggacg tgatgtgcca tttaacagtg
    32641 gtgtttttat ggatatggtc agttgcatca gccgggaact tgctcaccat gatattgact
    32701 tactactgat agctgatgat gagcatgccg attgccacag ctatatgcgg cttgttgaaa
    32761 gtcgcagggt tgatgctctc atcattgcac acactctgga tgacgatccc cgtatcacac
    32821 accttcataa agcaggtatt ccatttctgg ctcttggtcg ggtaccatct ggtctgccct
    32881 gtgcgtggtt tgactttgat aatcatgccg ggactggaca ggcaacccag aaactgattg
    32941 ctttgggaca taaacgtatt gcattgttga gtgaagacac ttcacatagc tatgttgttg
    33001 caagacgtca gggatggctt gatgcactga atgagcatga actggaagat acatggctgc
    33061 ggctggtttc tcccacacgg cgagcaggat atcaggccgt gatggagtta atgtcactac
    33121 cgataccacc aacagctatt attactgaca atgacctgag tggagatggt gctgccatgg
    33181 cactgcagtt gagcggtcgt ctttcaggaa aagatgcggt atctctggtt gtatatgatg
    33241 gtttgccaca ggacagtatt attgagctgg atgtggctgc agttattcag tcaacacgaa
    33301 gcctcgttgg tcgccagatt tccgagatga tatatcagat aattactgat tcatcatcaa
    33361 aaccactaca ggttgtctgg aacccggtat tttatccggg aaagacgatt catcctcctt
    33421 gctcctgatt tttttatcca gctcacatat ccgaaacgtt ttggttgata cccgaaacgt
    33481 ttcggatcaa cagtaaggca tccctgagaa aggagaatgt cttatggttt caaagtactg
    33541 cagactgagc agtcctcgct ctgatttaat tattaaaacc cgtccacatg cagaaattat
    33601 ctggtggggc tctgcactga aacatttctc accggatgac tgtgccagcc tggaaagacc
    33661 ggttgcgaat ggtcgtctgg atattgatac gccactaaca ctgatggctg aaaatgccct
    33721 gggacttttt agctctccgg gactggaagg acacaggaat gggctggatg catctcctgt
    33781 tttttataca gttgacgtgg aacataccga aaacactctg agacttacca gtgaagattc
    33841 ggtagccggc ctgcgtctgg tcagcgagct ggtgatgacg ccatcaggaa ttctgaaagt
    33901 tcgtcatgca ctgaccaacc tcagagaggg ggactggcag ataaatcgtt tcgccatcac
    33961 tttaccttta gcggaacgtg cggaagaagt catggctttt cacggacgct ggactcgtga
    34021 atttcagccg cacagggtac gtctcactca tgatgctttt gttctggaaa atcgcagagg
    34081 gcggacatct catgagcatt ttccggcgct gattgtcggc acgccaggct tctcggaaca
    34141 acagggagag gtgtgggctg tgcatctggg gtggagtgga aatcaccgca tgagatgtga
    34201 ggcaaaaact gacggcaggc gttacgttca ggctgaggct ctgtggatgc cgggtgagaa
    34261 ggctctcagg aagaatgaaa ccctgtacac cccgtggcta tatgcctgcc actctgcgga
    34321 tggcctgaat ggaatgagtc agcaatacca tcgttttttg cgtgatgaaa ttatccgttt
    34381 ccctgagcaa aaaccccgcc ctgtacatct caatacctgg gaaggtattt atttcaatca
    34441 caatcctgat tacatcatgc agatggctga gcgtgcagca gcactgggcg ttgaacgttt
    34501 cattattgat gatggctggt ttaaaggacg taacgatgac cgcgcggctc tgggcgactg
    34561 gtataccgat gaacagaaat acccgaacgg gctgatgccg gttattaaac atgtgaaatc
    34621 tctcggtatg gaatttggca tatgggttga gccggaaatg attaacccgg attctgacct
    34681 gtttcgtctt catcctgact gggtattgtc aatgcctgga tattcccagc caaccggaag
    34741 atatcagtat gttcttaacc tgaatattcc agaggccttt gcttacattt atgaacgttt
    34801 cttatggtta ctgggagaac atccggttga ttatgtgaaa tgggacatga atcgtgagct
    34861 tgtacaggca gggcatgaag gccgtgcggc agcagatgca cagacccgtc agttctatcg
    34921 attgcttgat ctcctccgtg aacgttttcc acatgttgag tttgagtcct gtgcttccgg
    34981 tggggggcgt attgacttcg aagtcctgaa acgcacacac cggttctggg catctgacaa
    35041 taatgatgcc ctggagcgct gcaccataca acgtggcatg agttactttt tccctcctga
    35101 ggtgatgggg gcgcatattg gccatcgccg ctgccatgca actttccggc agcacagcat
    35161 cgcttttcgt gggctgacgg cattgttcgg ccatatgggg ctggagctgg atccggtggc
    35221 cgcagatgcg aaggaatctg acggttatcg ccggtatgcc ttgctctata aagaatggcg
    35281 acaactgatt catacaggag ttctctggcg tgtggatatg ccagatcctt cgatacaggt
    35341 tcagggagtc gtcagccctg atcagtctca ggcacttttt atgatcagcc agcttgcaat
    35401 gccggattac accttaccag gcatacttcg ttttcccggt ctggcggcgg aagtgcgtta
    35461 ccggcttcgg gttattgatc acccggacct ccaggtggtt ggtgaaggcg gtcataccat
    35521 gcgcaaatta cctgtctgga tgaatcagag ccttgaggcc agtggtgaat ggctggcaca
    35581 gggagggatt cagctccccg tactggatcc ggagagtgcg attttgatag cacttgaaag
    35641 agctgtgtga agagaggtac ggagcctcag ctccgtcctg tttctcaata ttatcccgtg
    35701 agggtaagaa taatgaattc tgcaagtacg cataaaaatc ccgatttctg gattttcggt
    35761 ctgttcttct ttctctactt tttcatcatg gcaacctgtt ttccgttttt gcctgtatgg
    35821 ctgtccgatg tggtcggact gagtaaaacg gatacaggta tagtcttttc atgtctttct
    35881 ctgtttgcca tcagtttcca gccattgctt ggggtcatat cagatcgcct gggactgaaa
    35941 aaaaacctga tctggagtat cagcctgtta cttgtatttt tcgccccctt ttttttatat
    36001 gtatttgcgc ccctgctgcg cttcaatatc tgggcaggcg cactgactgg cggtgtcttt
    36061 attggttttg tgttttctgc aggtgccgga gctattgaag cttatataga gcgggtcagt
    36121 cgcagtcgtg gatttgaata cggtaaagcg aggatgttcg ggtgtctggg ctgggcgtta
    36181 tgtgcggcta tggctggaat gctttttaat gtcgatcctt ctctggtttt ctggatgggg
    36241 tcaggaagcg cattattgtt gcttcttctg ttgtttctgg cgcgccccag taccagccag
    36301 acggcaatgg ttatgaatac actgggtgcc aattcttccc tgatttcgac cagaatggtc
    36361 ttcagcctgt ttcgcatgcg tcagatgtgg atgtttgttc tctacacgat tggtgtggcc
    36421 tgtgtctatg atgtatttga tcagcagttt gccacatttt ttcgttcatt ctttgacact
    36481 cctcaggcag gaataaaagc attcggattt gctaccactg cgggggagat ttgtaatgcc
    36541 attatcatgt tctgtacacc atggataatt catcgcattg gtgccaaaaa taccctgctt
    36601 gttgcggggg gaattatgac catccgcatt accggttctg cttttgccac caccgcgaca
    36661 gaagtggtga ttctgaaaat gcttcacgct cttgaagttc catttttgct ggttggggcg
    36721 ttcaaatata ttacggcagt gtttgacacc cgactgtcag cgaccgttta tttaataggt
    36781 tttcagtttt ccaaacaact tgctgcaata cttctctcta cctttgccgg ccacctgtat
    36841 gatcgtatgg gattccagaa tacgtatttt gtgctcggga tgattgctct gactgttacc
    36901 gtgatatcag ttttcacgct gagttcttcc cgcgggagcg tacacccttc tgtagaaaaa
    36961 gcccctgcag cgcattcgga gattaactga tgaaacagcg tcttgctttg gcacagtctg
    37021 cccttgaaaa actttgcgca cgtcgtggta atgcctggta cccgattttt catctggctc
    37081 cacctgccgg ctggatgaat gatccaaatg gccttattta cttcaatggg cgttaccatg
    37141 cgttcttcca gcatcatcct gcaagcgcat atcaggggcc aatgcactgg gggcatgcca
    37201 ccagtaccga catgttgcac tggcaacacg aacctatcgc gctggcaccc ggagataaat
    37261 atgatcgtga tggctgtttt tcagggagtg ccgtggatga tgatggcgtg ctatcactta
    37321 tttatactgg tcatatttgt ctcgatgatc gtggtaatga cagcattatc cgtgaagtac
    37381 agtgtctggc tacaagtcat gatggtattc actttgagaa gcagggctgt gtgctgacac
    37441 ctccggaagg tataatgcat ttccgtgatc ccaaagtctg gcacgaagac ggctcctggt
    37501 ggatggtcat tggtgcccgg gacgcttctg acaatgggca agttctgttg tatcgcggga
    37561 catctttgcg ggactggcat ctggagcatg ttcttgctca ttccgcagcc ggaaaaagtt
    37621 atatgtggga atgccccgat ttcttcaggt gtggtaattt tcactggctg atgttctcac
    37681 cacaggggat gcccccttcc ggttatcggt tccgtaacct ttttcagagc ggtgtgttgg
    37741 cagggagctg gaagcctggt tctgtctttg cgctgaaagg gggatttgaa gagctggatt
    37801 atggtcatga cttttatgct ccacagtcca tgctggctga ggacggcagg cgtatcatta
    37861 tggcatggat gaatatgtgg gattcacccg tgcccacccg cagtgaagcc tgggcaggat
    37921 gtctgacgct gcccagagag gtttttgagc gcgatggccg gctgtgccag cgacctgtgc
    37981 gtgaagtcga atctctgcgc aaaaaatgcc agccattatc ccctgtaagg ttacagggtt
    38041 tgcaattact gaccgaaaat gtacaggccg cagaattatt ggtgacgtgg catacggttg
    38101 acagtcatgc ggagcactat ggcgtccgcc ttggagacgg tctgcgactt tatgtggata
    38161 atcaggccgg gcgattggta ctgtggcgct attaccctga ggaagggttg gatggttacc
    38221 gcagtgttga acttcctgat acagaatatc tgactcttcg tattttcttg gatcgttcat
    38281 ctgttgaagt gtttgttaac gatggtgagg caaccttatc aagtcgtatt tatccgcaag
    38341 cggactcgag acaattatcg ttatatgccg ctcatggcga tgcgatatta actgatggca
    38401 ctttatggat gctgacctga gacatatcga tatcagtatt ttttctgaca ttatctgtca
    38461 ttactttgcc agtacacatt aattatcttt gctgtgtgct tttggtaatg tgttgcgatt
    38521 atatatacca gtctggtgaa gaatatgaat ggtattttgt gttgatacag agaatataag
    38581 agtacccaca tttgttttta tttaaggggg taaaatgaat aagttacttt tgggtttatc
    38641 tcttttttta ttactggaat acaatgcgtg tgcagcaaaa acaggggggc taacgcttga
    38701 acaaagaatg gcattgctgg aagaacgcct ggaagctgca gaaaaaaggg cggaaaaagc
    38761 agaaagaatg ttaaagagtt ttgatataga acaacattct gaaatacgtc aaattagttc
    38821 agagcagaat aaaaaagatg ctaacagcta tgcagtagtt gagtcaacaa aagaaaaaac
    38881 atcttctcca ggttttctgc tctctggtta caatgatttg aagttttatg gtgatgtgga
    38941 gtttaatata gatgctgcca gtaaacccgg tcagttagta atgataagtt ccggggcgaa
    39001 cagtgagtca gtgaatgaac ggtgggacct taatggccgt attctattag gttttgacgg
    39061 tacccgtaag cttgataatg gttatttcgc tggattttca gcacaaccac tggcggatat
    39121 gcacggttca gtgaatattg atgatgcatt attctttttt ggaaaagatg acgagtggaa
    39181 agtgaaagtc ggtcgttttg aagcttacga tatgttccct ctaaatcagg atactttcat
    39241 tgagtattcc ggtaatacag ctaatgatat ttatgctgat ggccgtggtt atatctatat
    39301 gatgaaagag ggacgcggtc gttctgacgc tggtggtaat ttcctcatca gtaaacaact
    39361 cgataactgg tattttgagt taaacacgtt acttgaagac ggaacatctt tatataataa
    39421 cggtaattat catggacgat atatggagca gcagaaaaat gtcgcttatc tgcgcccggt
    39481 aattgcctgg tcgccaacgg aagaattcac agtctccgca gcgatggaag cgaacgtggt
    39541 aaaaaatgct tatggctata ccgataataa ggggaatttt gtcgatcagt ccaatcgttc
    39601 cggttatggt attagcatga catggaacgg tctgaaaaca gatccggaaa atggcatcgt
    39661 ggttactctt aataccgcct atttggatgc cagtaatgag aaagatttca ctgccagtat
    39721 taatgctctg tggaaacgtt tcgaactggg ttatatctac gcacataata agattgatga
    39781 atttagtggt gtcgtgtgta ataatggttg ctggattgat ggtgaaggaa tatacaacat
    39841 tcacaccatt catgcatctt atcagtttgc taatgtgatg gatatggaga actttaatat
    39901 ttacctcggg gcttattact caattctgga tagtaactgt agatatagta attgtgacat
    39961 taccgatgat cgttacggtg cccgattacg tttcaaatac tttttttgat gcatagtaac
    40021 aaagtattgt tccacactta cattaacgcg tttttcgggc gcgtcatcag cgatgcatac
    40081 gaatgatcgc agtctgccaa tggtcgattt atcttggttg cgcaggggcg agcaccgggc
    40141 aggtaccggc ggctgactct gaagctctga gtcgggtaat tgcgtcaggt cacgaaggac
    40201 tgacacgggt attggtggca ggtgatgaag tactgagtgt ggtaatttcg gggatgcaat
    40261 gcaggagctg agtcaggttt gcactgagct gaggacccgt agcgagcggg ttcctgctat
    40321 ttttcggtgt caggggcgca agtcccgacg tcgggacttg ccgggagatt gtcaggtcat
    40381 tgaccgggtg tgagcggctc gacgtaagcg gtagatatgc ccaattcccc ccgataaatt
    40441 cattaccaaa cacattattc aatttgtttt tatttttccg ctttcagtca ttatcgctat
    40501 cgcttcgtcg gtaatgactc caacttattg atagtgtttt atgttcagat atgcccgatg
    40561 actttgtcat gcagtggatt tgcccctata tttccagaca cctgttatca cttaacccat
    40621 tactggcccg ctgccgtaga tattcccgtg gcgagcgata acccagtgca ctatgcggat
    40681 gccattcgtt ataatgctcg aacgcctctg caaggttctt tgctgccatt aacccgtctg
    40741 gtttgggcat gatactgatg taatcacgct ttatcgtttt cacgaagctc tctgctatgc
    40801 cgttactctc cggactccgc accgccgtgt tcttcggttc aagtcccaac atccgggcga
//
LOCUS       DAJOSY010000027        40115 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJOSY010000027 DAJOSY010000000
VERSION     DAJOSY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.027"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(90..719)
                     /locus_tag="OHN27_001592"
     CDS             complement(90..719)
                     /locus_tag="OHN27_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCQ2286051.1"
                     /translation="MNWQLISFFGDSTVLLPSAAALFIVLMLRKTSRLLAWQWSLLFG
                     ITGAIVCASKLAFMGWGLGIRELDYTGFSGHSALSAAFWPIFLWLLSARFSAGLQKAA
                     VATGYILAAVVGYSRLVIHAHSVSEVIAGLLLGAVGSALFLLLQKRTTHSENVRISWG
                     GVACLVMVPLILLHSGNKAPTQSLLGQIATAAGPLDKPFTRTDLHKQAW"
     gene            complement(1113..2003)
                     /gene="galF"
                     /locus_tag="OHN27_001593"
     CDS             complement(1113..2003)
                     /gene="galF"
                     /locus_tag="OHN27_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GalU regulator GalF"
                     /protein_id="HCQ2286052.1"
                     /translation="MANLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEIVLVTHSSKNAVENHFDTSYELEALLEQRVKRQLLAEVQAICPPGVTIMNV
                     RQAQPLGLGHSILCARPVVGDNPFVVVLPDIILDGGTADPLRYNLAAMVARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPMVAEGQVARIVEFIEKPDEPQTLDSDLMAVGRYVLS
                     ADIWAELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGESYDCGKKMGYMQAFVTY
                     GMRNLKEGTKFRESIKKLLA"
     gene            2756..3790
                     /locus_tag="OHN27_001594"
                     /pseudo
     CDS             2756..3790
                     /locus_tag="OHN27_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            3863..5446
                     /locus_tag="OHN27_001595"
     CDS             3863..5446
                     /locus_tag="OHN27_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCQ2286053.1"
                     /translation="MEWIADPSIWAGLVTLVVIELVLGIDNLVFIAILAEKLPPAQRD
                     RARITGLMLAMVMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAIGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEAFNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(5898..7751)
                     /gene="asmA"
                     /locus_tag="OHN27_001596"
     CDS             complement(5898..7751)
                     /gene="asmA"
                     /locus_tag="OHN27_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCQ2286054.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVCADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLMA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGLSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPMLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(7773..8354)
                     /gene="dcd"
                     /locus_tag="OHN27_001597"
     CDS             complement(7773..8354)
                     /gene="dcd"
                     /locus_tag="OHN27_001597"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCQ2286055.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(8446..9087)
                     /gene="udk"
                     /locus_tag="OHN27_001598"
     CDS             complement(8446..9087)
                     /gene="udk"
                     /locus_tag="OHN27_001598"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCQ2286056.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            9405..12721
                     /locus_tag="OHN27_001599"
                     /pseudo
     CDS             9405..12721
                     /locus_tag="OHN27_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(12759..13616)
                     /gene="alkA"
                     /locus_tag="OHN27_001600"
     CDS             complement(12759..13616)
                     /gene="alkA"
                     /locus_tag="OHN27_001600"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCQ2286057.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            13750..15102
                     /gene="yegD"
                     /locus_tag="OHN27_001601"
     CDS             13750..15102
                     /gene="yegD"
                     /locus_tag="OHN27_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCQ2286058.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPDAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFHSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALNQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(15115..17055)
                     /gene="yegI"
                     /locus_tag="OHN27_001602"
     CDS             complement(15115..17055)
                     /gene="yegI"
                     /locus_tag="OHN27_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCQ2286059.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATIHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFIGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLTPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHIYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQVEPRYIILVPVLAAIWIIGSLTSKAYKAEVQRRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALQDNARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRSVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQTVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(17052..17813)
                     /gene="pphC"
                     /locus_tag="OHN27_001603"
     CDS             complement(17052..17813)
                     /gene="pphC"
                     /locus_tag="OHN27_001603"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCQ2286060.1"
                     /translation="MSWRLVYASAVGTSHISADLSCQDACQMQVAWLEDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDDDAVSRLETYTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(17810..18469)
                     /locus_tag="OHN27_001604"
     CDS             complement(17810..18469)
                     /locus_tag="OHN27_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCQ2286061.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAHGDTPMGAAITK
                     ALNMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(18691..18750)
                     /gene="ibsA"
                     /locus_tag="OHN27_001605"
     CDS             complement(18691..18750)
                     /gene="ibsA"
                     /locus_tag="OHN27_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCQ2286062.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(19023..19079)
                     /locus_tag="OHN27_001606"
     CDS             complement(19023..19079)
                     /locus_tag="OHN27_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCQ2286063.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            complement(19351..19407)
                     /locus_tag="OHN27_001607"
     CDS             complement(19351..19407)
                     /locus_tag="OHN27_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCQ2286064.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(19679..19735)
                     /locus_tag="OHN27_001608"
     CDS             complement(19679..19735)
                     /locus_tag="OHN27_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCQ2286065.1"
                     /translation="MMKILIIVVLLVVSYPAY"
     gene            20014..21261
                     /gene="mdtA"
                     /locus_tag="OHN27_001609"
     CDS             20014..21261
                     /gene="mdtA"
                     /locus_tag="OHN27_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCQ2286066.1"
                     /translation="MKGSYKSRWVMVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARFQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            21261..24383
                     /gene="mdtB"
                     /locus_tag="OHN27_001610"
     CDS             21261..24383
                     /gene="mdtB"
                     /locus_tag="OHN27_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCQ2286067.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDLMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCAWMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWAK
                     SRFARHEEEA"
     gene            24384..27461
                     /gene="mdtC"
                     /locus_tag="OHN27_001611"
     CDS             24384..27461
                     /gene="mdtC"
                     /locus_tag="OHN27_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCQ2286068.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            27462..28877
                     /locus_tag="OHN27_001612"
     CDS             27462..28877
                     /locus_tag="OHN27_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2286069.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            28874..30277
                     /gene="baeS"
                     /locus_tag="OHN27_001613"
     CDS             28874..30277
                     /gene="baeS"
                     /locus_tag="OHN27_001613"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCQ2286070.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRSILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASSLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            30274..30996
                     /gene="baeR"
                     /locus_tag="OHN27_001614"
     CDS             30274..30996
                     /gene="baeR"
                     /locus_tag="OHN27_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCQ2286071.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            31187..31519
                     /locus_tag="OHN27_001615"
     CDS             31187..31519
                     /locus_tag="OHN27_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCQ2286072.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            31642..33042
                     /locus_tag="OHN27_001616"
     CDS             31642..33042
                     /locus_tag="OHN27_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCQ2286073.1"
                     /translation="MLKGLRTLFSRRVLFNVEMNNSVDCELLLIQQQLQALIAEPGNY
                     LAHDCDLNDTLVLCQLTHHHNGYIREKAVQYLGHKEDVAAIEELLLAANDWVKEVRLA
                     ATIALRHLMKDKNAAAFAVNLPMIRNLLQCQRYDHRAFVDEILRYLLTENNRPQLTYW
                     LTCPDKKLSRAVLQTLIEYGYFNDESSLLSILDKPDEGLRMLATTHWLRQQLPLSEAV
                     LTRLLKDRWPRIRQATLFSLTDRAIEMPPALHSTLLLDNNMLIRLRAKNMLHEVMDVP
                     QFWRHVVTSAEYTPSQRRAALYGLDSIHDPNILKLAEWGLSQNVFPLRLAAMHILAKA
                     NPRCGVKETILTTLANPDAAGLRFMVNICVWCRVPLTFEEIRQLQENAPSVKHACAYC
                     RLYHNLNKWDGLILLLQSQHKLTEEFAGKQLAIWQRNFNLSGIQPNALQRQQLQALFT
                     RNPELHNRLWGYIPFK"
     gene            33363..34724
                     /gene="yegQ"
                     /locus_tag="OHN27_001617"
     CDS             33363..34724
                     /gene="yegQ"
                     /locus_tag="OHN27_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCQ2286074.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     APQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(35054..35371)
                     /locus_tag="OHN27_001618"
     CDS             complement(35054..35371)
                     /locus_tag="OHN27_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286075.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            35785..36684
                     /gene="yegS"
                     /locus_tag="OHN27_001619"
     CDS             35785..36684
                     /gene="yegS"
                     /locus_tag="OHN27_001619"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCQ2286076.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(36766..37539)
                     /locus_tag="OHN27_001620"
     CDS             complement(36766..37539)
                     /locus_tag="OHN27_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2286077.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(37645..38685)
                     /gene="gatD"
                     /locus_tag="OHN27_001621"
     CDS             complement(37645..38685)
                     /gene="gatD"
                     /locus_tag="OHN27_001621"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCQ2286078.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVASVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(38733..40088)
                     /locus_tag="OHN27_001622"
     CDS             complement(38733..40088)
                     /locus_tag="OHN27_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCQ2286079.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKLSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
BASE COUNT         9552 a        10641 c        10273 g         9649 t
ORIGIN      
        1 ttatcaattg actggcatat agtagattat tttcttattt gtccaattaa ccggcgaaaa
       61 cttaatttcc cctgtaaaac aaatggcgat taccaggcct gcttatgcag atcggtacgc
      121 gtaaaaggtt tatccagcgg ccctgctgca gtggcgattt gccccagcag ggattgagtc
      181 ggcgctttat taccgctatg caacaggatg agcgggacca tcaccagaca cgcgacgccc
      241 ccccaggaaa tacgcacgtt ttccgaatgg gtggtacgtt tttgcagcaa caggaacaaa
      301 gcgctgccga cagcgccgag cagcaggcca gcgatcactt ccgagacgga atgcgcatgg
      361 atgaccaacc gtgaataccc caccacagcg gccagaatat agccggtggc gacggctgct
      421 ttttgcaaac ccgcggaaaa acgagcacta agcagccaca ggaagatagg ccagaaggcc
      481 gcggaaagcg ctgagtggcc gctgaagccg gtgtaatcca gctcgcgaat acccagcccc
      541 cagcccataa aggccagttt ggaggcgcag acgatggcgc cggtgatgcc gaacagcaaa
      601 ctccactgcc acgccagcaa ccgtgacgtt ttacgcagca tcaggacaat aaacagcgcc
      661 gctgcgctgg gcagcaaaac ggtgctatcg ccaaaaaatg agatgagttg ccagttcata
      721 tacctgtgat ccttcgatga tgtccactgc cgtgctcagg gattatccgt aatgtctgta
      781 ccagctcgca tgccaattca tttcacattt ttatgcatgc aagtgagata tttaaagcgg
      841 aaacatgatc gcagtcaagt tttatttttg gctgacgttg accgctttcc ctgcgcttta
      901 ggaattgtcg cactaagtga ctattcacaa agcattattg ccgctactcc gaccattgca
      961 ccacagaggc cagaggcaaa ctaccagaag tctccgaagc catacgtttc tttacaatcg
     1021 ttataacatt cacgttatga ataatttctg cataacgtta tgagcggcgt ggcaaaatac
     1081 ccctgccctg ccgctcactc ttacgccggc gctcaggcca gcaatttttt aatgctttcg
     1141 cggaatttgg taccttcttt caggttacgc atcccgtagg tgacgaaggc ctgcatgtag
     1201 cccatttttt taccgcagtc atagctttcg ccggtcatca gcatcgcgtc aacggactgc
     1261 ttcttcgcca gttcggcaat ggcgtcggtt agctggatac ggccccaggc gcccggctgg
     1321 gttcgttcaa gttcagccca gatatctgcc gacaacacgt agcggcccac cgccatcagg
     1381 tcggagtcca gggtctgtgg ttcgtccggt ttttcaatga actcaacgat acgcgccacc
     1441 tgcccttccg ccaccatggg ctctttggtc tggataacgg agtattcgga gagatcgccc
     1501 ggcatgcgtt tcgccagcac ctgactgcgg ccggtctcgt taaaacgggc gaccatggca
     1561 gcgaggttat agcgcagcgg gtccgcagtg ccgccatcga gaatgatatc cggcagcacc
     1621 accacaaacg ggttgtcccc gactaccggg cgggcgcata ggatggagtg gcccagaccc
     1681 agcggctgtg cctggcgcac gttcatgatg gttacacccg gcgggcaaat ggcctggact
     1741 tccgccagca gctggcgctt cacacgctgc tcaagcaggg cttccagctc gtaggaggta
     1801 tcaaagtggt tttccaccgc attcttcgag gagtgcgtca ccagaacaat ctctttgatc
     1861 ccggcggcga cgatttcgtc aacgatgtac tgaatcattg gcttatcaac gatcggcagc
     1921 atctcctttg gaattgcctt tgtggccggc agcatatgca tgcctagtcc tgcgaccgga
     1981 ataaccgctt tcaaattcgc catattcatc ttcacctgtt tggttgcaaa acacgcggtt
     2041 ttgcaaaatt gtacatgtca caaattcatt gaaactgata atacacggat actgcccgag
     2101 catatgcccg ccaaattctt aaattcactg tcacgtaaaa gaatacacac aactcccttt
     2161 taccaacagt taaaactgag aataatctta ttctcgttca ggttattcag cggctcgttc
     2221 ctttgctccg cacgatgtgg ctgagatgcc cgtctatcgg gacctgttgg acgccggacc
     2281 cgccgcgcgt atgcccgaaa agaatatata gataataaat ttcgccaggc aatatacttc
     2341 agtataaaca gccggatatg acgacatatt agcctgaatc cgcggctgtg aactgcgatc
     2401 caccctattc tgcactcact tcataatatg aattatgccg gttttgtgag cgggcttcgc
     2461 gcttcgcttc tgctctgcgc atcagcgctc accgtgacga accgcgcgag taagcccttt
     2521 tgccaccctc gctgaaacgc agcggataaa gttctgatat cggaaagctt attccatggt
     2581 cgccgccatg gtgttatttt ttcactgttc ttttccggaa cataattctt atcggttcgc
     2641 ttaatccgct ggaaatatat ccacccacgg cagagcgatg tccgcgtcgt tattgcctgt
     2701 ataagttcac agagatattg caattgcctc tggataagta aggggagatt gcactatgca
     2761 aatgcagcac ctgatggttg gctatcctaa gtactaccaa acagccgatt atgcgttgag
     2821 gctttcagtg atggctgata tagcaacaat gagaatgaag gctctgcact tctgggataa
     2881 acacgatatt tctgcagcgt ctgaagcctt tggcgtatcc tgccgcacgc cttactggtg
     2941 gcgccagtta ctgaacaagg gaggacctga ggggccaatc ccgcacagta aggcacctct
     3001 ggtgcgacga aaaaagcact ggcatcccga tgtgctgaaa gagattcgac gcctcaggac
     3061 agagctgccg aacctcggta aagagcagat ttttgttcgc ctgaagccct ggtgcgtaca
     3121 acgccacctg acctgcccga gtgtttccac catcggcaga atgatcgcca ccgcacacga
     3181 taaaatgcgg atgataccag tacgtctgag ctcgcggggt aaggctctgt ttgtcaaaaa
     3241 gcgaaccacc aaaccccgca ggccaaagca ctatcgtccg gtaaagaccg gagagctcat
     3301 tgggatggac gccattgagc tcagaatggg tgaaatgcgt cgctatatca ttaccatgat
     3361 cgacgaacac agcgattacg cactggcgct ggctgtgccg tcgctcaaca gtgatatcgt
     3421 caatcacttt ttcagccgtg cagcccggct gtttcctatc ggtatcagcc agatcatctc
     3481 agataacggg aaagagttcc tgggaagctt tgacaaaacg ctgcaggaag ccgccatcaa
     3541 acacatctgg acctatccct acacgccaaa aatgaacgct atctgtgatc gttttaaccg
     3601 gacattaaga gagcaattta ttgagtttaa tgaaatttta ctctttgagg atctggcgtt
     3661 gtttaaccag aagctggcgg aatatctggt gctgtataac agcaaaagac cccacaaggc
     3721 gctcgcacta atgacgcccg tggaatatat gttaaaagag aacaaaaatt gcaatatgtg
     3781 gtggacccat acattgcatt ttagactaat cttatccatg atcgtgataa cacatactgc
     3841 agtcgtacag gttttaatac atatggaatg gattgccgat ccgtcaatct gggcaggtct
     3901 tgtcacgctg gtggttatcg aactggtgtt gggtatcgat aacctagtct ttatcgctat
     3961 cctcgccgag aagctccctc ccgcccagcg cgaccgagcg cggatcactg gcctgatgct
     4021 ggcgatggtg atgcgtctcc tgctgctggc ctctatctcc tggctggtca cccttactca
     4081 accgctgttc agcttccgct cgtttacctt tagcgcccgc gacttaatca tgctgtttgg
     4141 tggtttcttc ctgttgttca aagccacgat ggagctgaac gaacggctgg aagggaaaga
     4201 tagcaataac cccacgcaac gcaaaggtgc gaagttctgg ggcgtggtga cacaaatagt
     4261 ggtgctggac gctatcttct cactcgactc ggtgattacc gccatcggga tggtcgacca
     4321 tttactggtc atgatggccg ccgtggttat cgctatcagc ctgatgttga tggccagcaa
     4381 gccgttaacc cagttcgtta acagtcatcc gacgatcgtt attctctgct taagcttcct
     4441 gttgatgatt ggctttagcc tggtggcgga aggtttcggc ttcgtcattc cgaaaggcta
     4501 cctgtacgct gccattggtt tctcggtgat gatcgaggcg ttcaatcagt tggctatctt
     4561 taaccggcga cgttttcttt ccgctaacca gacactgcgc cagcggacta ccgaagcggt
     4621 aatgcgcctg cttagcgggc aaaaagaaga tgcggaactg gacgccgaaa ccgcgtccat
     4681 gctggtggat catggtaacc agcagatctt taatccgcag gaacggcgga tgattgagcg
     4741 ggtacttaat cttaaccagc gtaccgtcag cagcattatg acgtcgcgcc acgatattga
     4801 gcatatcgat ctcaacgcgc cggaagacga gatccgccag ttgctggagc gaaatcagca
     4861 tacgcggctt gttgttactg atggcgatga cgcagaagat ttgctcggtg ttgttcacgt
     4921 tatcgacctg ttacaacagt cactgcgcgg cgaaccgctc aacctgcggg tgttgattcg
     4981 tcagccgctg gtgttcccgg aaaccttgcc gttgttacct gccctggagc agttccgtaa
     5041 tgcccgcaca cactttgctt ttgtggtgga tgagtttggc tcggtggaag ggattgtgac
     5101 attaagtgac gtcactgaaa ccattgccgg taacttaccg aacgaagtgg aagagatcga
     5161 cgcccgccat gatattcaga agaatgcgga cggttcctgg acggcgaatg gtcatatgcc
     5221 gctggaagat ctggtgcaat atgtgccgct gccgctggat gaaaaacgtg aatatcacac
     5281 cattgccggg ctattgatgg aatatttgca gcgtattcca aaacctggcg aagaagttca
     5341 ggtgggggat tatttgctta aaacgttgca ggtagaaagc catcgcgtgc agaaggtgca
     5401 gattattccg ctgcgtaagg atggcgagat ggagtacgag gtgtgatggt atcggccatt
     5461 ttacccaatg ctgcgtaacg ccttataact tcagcaatga agcgttgtct gtggtacatg
     5521 gacatcacag cgggggtgtg ggtccggctg taaatcgccg aatcgtttcc tgtaggccgg
     5581 ggcagcccac atggatgtgg gctgagggcg cgttttacag tgatgttacc tcgcgcccgt
     5641 acccgatagc cgaaaggaat aagatgaggg aaccacgcca gcggcgattt accgccggga
     5701 gcccgggttg ccagggtggt ggcgattgag ccaccctggc acgttcaccg gcttgagcgt
     5761 tccatagtag cgaggaacat caagtgaacg gaacagcctt cgatcttaca tattcagaat
     5821 ataatggtgc cgccacgaac gcctgatgcg acgctttacg cgtcttatca ggcctacagg
     5881 acaggtcacc tggtaaccta catcttctcc agcaacttct tcacatcttt gccattgcgg
     5941 gaatctttat tccgctcggc ccagtcgtta agacggcgtt ttgcttcatc ctgtagatgt
     6001 ttacgcagta actgatccac ttgcaggctg taattaagtt gctgccagtt gccataaacc
     6061 cgcagcggta ctggcgtttc tttgaggaaa tcaatcagtt tgctttctcc gttccagcca
     6121 cccacgaccc gaatatcaaa ctgggtgtcg caggtttgct ccgccagatt caacgtacca
     6181 gccccggaaa gcgccagcat gggcgattga ccttgcatgt cgtttaacgt cacgacgcca
     6241 tccttcaacg tcaaatcggt ggtaaagcgg tcaagacgcg tcacgttatc gaagttttca
     6301 gcggccttca catcaccacc attacgttct accgcttgct gaatcatctg ctggaagttc
     6361 atcccttcca tgcgtgtgtc ggtcatttcg acatgtgcct gtccttgcca gttgtggcgg
     6421 aatgcgtcgg catctatgtc agcaccggag aaatcaccag ccagtgacat ctttccggtc
     6481 aacgaaatcg gatagttaaa cgccttcaga atggtgccaa tctcaacgtt ttccagccgt
     6541 ggctggaagt ttatccgcgg atttattgat gtcgcgtcca acgtgcccgg cagtgaaacc
     6601 tgtccaccgt taagtttgcc ctgcagttga gtaatttcca gcaaacccga cttgttggtc
     6661 atttgcgtgg caacatctgt aaaattcatt ccgcgccagc gcacgttact ggcctgcaac
     6721 aaaatatcag ccgtaaagcc ttgcagtccc tgataggccg gttcatcaat acgcgaagaa
     6781 atgaccgggc gcggcaacgt tgattggctc agcccttgct gcgcggcacc gttctcacca
     6841 ttcgctgttt cattaagggg gatgaggttg tcaagattca gttgcgggaa ttgcagtctc
     6901 agctgccatt caggtttctc tgtcaacgtg acctgtgctt gcccgctcag cgtactgtca
     6961 ttggcggtca aactaatttg gttaaatgaa aggcgtttat gtgactcctg ccactggacc
     7021 tggaaactcc cctgcccctg aataccttgt tttggtaaat cggcaccctg caactgccag
     7081 ttaatttgtt caatagccgc cattaaatca tgcggataat cagaagcatc caccgtaccg
     7141 ttaagggata tcgtgagatc gcgctgatcg cgattaaccc gcccggagaa ctcaaatgag
     7201 ccacgatgtt ggggatcttg ttccatttgc aggcggatat tgcggattgt cacctgctcg
     7261 tcatcttcat gctggaacac cagaacgctg tccgccacct taagactgga tatatcaaac
     7321 gaccatccgc gatcgtctga cagatccggc aaggtattgt cgcgcggtgc aaccggagcg
     7381 tcttcactgc gcacagcttc cgtctgcggc gtcagttgga tcactgcccc ctttagcatc
     7441 acctgcttaa cgctcagttg atgactcagt agtggtaaaa gcgccacgtc cagacgcatg
     7501 ttgtcggcgc aaaccagtgg ctggcttgcg ccctgggcgg tgagagacat tcgcccggag
     7561 aggatactaa gctgcggcca gacgtgccaa cgcagtggcc cgtcgagctg caattgataa
     7621 ccgctacgtg cagcaacttg cttgaccata tagtcgcgga aatcattcgg attcaccagc
     7681 aatactaacg cagataaccc ggccaccagc acgaccagga gtatcatcag cgtcgtcaga
     7741 aatcgtctca tggtatcctc aatgggcctg aattagtctt tatcgattcg gctggctacc
     7801 gcgccctgct ggttgcgata tttcgcatct tcgcggcggt tgtaaggtcg cgccgccggg
     7861 ccggaaagcg gctcaaagct cagcgcacca attaacatgc ccggacgcag cgccagcggc
     7921 agcttaccgg aattgtagaa ctccagcaca atgcagccag accagcccgg atcgatacgg
     7981 tgcgcggtga cgtgcaccat cagccccaga cgcgccagtg aggaacgccc gtccagccag
     8041 cccaccagat cggctggcag cgtcaccgac tccagcgtca ccgccagcgc cagctctcct
     8101 gggtgaagat agaacgcctc gctttcatcg agaacgatct catcgctcat cacgcggtca
     8161 agcgcggcgc tcacttcatc tttgggaccg ctcagatcga taaacgctgc cgtgtgacca
     8221 cggaaggtac gaaatttatt gcccaggcgc acatccaccg tcgcgccatt aatacgctcc
     8281 actggcggac gtgggttgat cgacaaacgg ccttcatcaa gccaggcttc aatatctcgg
     8341 tcacacagac gcatggcatt ttctcctttc gcgcatcact cccttaacgc caattacgtc
     8401 aagggcatac caggttatca ctgaacggta cacaatttat caagcttatt caaagaactg
     8461 acttattttc gctttcaata tatcgatcgc gatgcggttt ttcccgccgc gcggcacgat
     8521 aatgtccgca tattgtttag aaggctcaat gaattgcagg aacatcgggc gcacggtttt
     8581 ttgatattgc gccatcactg aatccattga acgcccacgc tcgttaacgt cacgcttgat
     8641 gcggcgcatc aggcagatat ccagcggggt atcaacgaaa atggagaagt taagttcgtc
     8701 acgcaaacgc gcatctgtca gcaacaaaat gccttcgaga atgatgacct tcttcggctc
     8761 aaccgtcacc gtttctttca tacgcgtatg ttcaacatag ctgtaaaccg gcaggtcaat
     8821 tgccgagccg cgtttcaacg cttgtaaatg ctcaagcagc aggctgtgat ccatcgcgct
     8881 gggatggtcg tagttggtct taacgcgttc ttccatcgac agatggcttt gatctttgta
     8941 atagcagtct tcgggaatta cgccgatgtg ttcatcaccg acttgctcac gcaattcacg
     9001 ataaagggta ctggcaataa gactcttgcc ggaagccgat gcgccagcga taccgataat
     9061 gacgcactga tgagactgat cagtcatata tttagcgacc tgattaacct ggatgttagg
     9121 aagggggcga cgaagcgcca aacgcggcaa ttatagggat ttcatccgtc tgataccagt
     9181 cgaatagcgt tgccgcgcgc tcagagttaa ttgttgacga agaattcccg gtggcaaatt
     9241 acgttgatca gttttataca aggtaaaaaa tgttatacgc agttgcgcaa attatccgcc
     9301 tttacgtcac tttatgagca attcgcatat aaaatgtaaa acttttgtac tagcataaac
     9361 acagaaacga atactggcga cctggtcttg cggataaagc ggtaatgagc aaacaatcac
     9421 agcatgtatt aattgccctg ccccacccgc tgcttcacct ggtcagttta ggtttagtct
     9481 cgtttatctt tacccttttc tcgcttgagc tttcgcagtt tggcacccaa ctcgccccac
     9541 tgtggttccc gacgtccatc atgatggtgg cgttttatcg ccatgccggg cgcatgtggc
     9601 cgggaattgc gctgagctgc tcgctgggaa atatcgccgc atccatcctg cttttttcca
     9661 ccagctcgct gaacatgacc tggacgacca tcaatattgt tgaagccgtg gtcggggcag
     9721 tgctgctacg taaattgctg ccgtggtata accccttgca aaatctggct gactggctgc
     9781 gtctggcact cggcagcgcc attgttccgc ctctgttagg gggtgttctg gttgtcctgc
     9841 tgacgcccgg agacgatcct ctcagggcat ttttgatatg ggtactgtca gaatccatcg
     9901 gcgctctggc actggtgccg ctgggattgt tatttaaacc acactatctg ctgcgccatc
     9961 gcaacccacg gttgcttttt gagtcgctgc tcacgttagc catcacactg acgttaagct
    10021 ggctttcgat gctgtatctg ccgtggcctt ttactttcat tattgtgctg ttgatgtgga
    10081 gcgccgtgcg cctgccacga atggaagcct ttttgatctt ccttaccacg gtgatgatgg
    10141 tgtcactgat gatggccgcg gatccctccc tgcttgctac gccgcgtaca tacctgatga
    10201 gccatatgcc gtggctaccg tttttgctga tcctgctgcc cgccaacatc atgaccatgg
    10261 tgatgtatgc ctttcgtgcg gaacgcaaac acatttccga aagcgaaacc cgctttcgga
    10321 acgcgatgga atattccgcc atcggcatgg cattagtggg caccgagggg caatggctgc
    10381 aatccaacaa agcgctctgc cagtttctcg gttacagtca ggaagaactg cgcggactca
    10441 cctttcagca actgacctgg ccggaggatc tcaataaaga tctgcaacag gttgaaaagc
    10501 tgatcagcgg tgaaataaac acctattcaa tggaaaaacg ctactacaac cgcaatggcg
    10561 atgttgtctg ggcgttgctt gccgtctcac tggtgcgcca caccgatggt acgccgctct
    10621 attttatcgc tcagattgaa gacattaacg agctaaaacg caccgaacag gtgaatcagc
    10681 aactgatgga gcgcatcacg ctggctaacg aagctggcgg gattggcatc tgggagtggg
    10741 agcttaagcc gaatattttt agctgggata agcggatgtt cgagctgtat gaaattcctc
    10801 cgcatatcaa accgaactgg caggtgtggt acgagtgcgt gctgccggaa gatcgccagc
    10861 acgccgaaaa agtgattcgc gattcattgc aatcgcgctc tccctttaaa ctggaatttc
    10921 gcattgccgt gaaagatggc atccgccata tccgcgccct cgccaaccgg gtactgaata
    10981 aagaaggcga agtcgaacgt ctgctcggca ttaatatgga tatgaccgag gttaaacagc
    11041 ttaacgaggc attgtttcag gaaaaagagc gcctgcacat tacgcttgat tccatcggtg
    11101 agccgtggtc tgtattgata tggcgatgaa aattaccttt atgaatccgg tggcggagaa
    11161 aatgagcggc tggacgcagg aagaagcgtt aggtgttcca ctcctgacgg tgttgcatat
    11221 tacttttggc gacaacggac cattaatgga gaacatttac agtgccgaca cctcacgttc
    11281 cgcgattgaa caagatgtgg tgttgcactg ccgaagcggc ggcagctacg acgtgcatta
    11341 cagtattacg ccgttaagta ctctggacgg cagcaatatt ggttcggttc tggtgattca
    11401 ggacgtcacc gaatcgcgca aaatgctgcg ccagctgagc tacagcgcct cccatgatgc
    11461 actgacgcat ctcgccaatc gcgccagttt tgagaagcaa ctacgcatcc tgctgcaaac
    11521 ggtaaacagt acacatcagc gacatgcact ggtgtttatc gatcttgatc gctttaaagc
    11581 ggtgaatgac agcgccgggc acgccgctgg cgacgctttg ttgcgcgaac tggcgtcatt
    11641 gatgctgagt atgctgcgct ccagcgacgt gctggcgcga ctcggtggcg atgaatttgg
    11701 tctgctgctg ccagattgta atgtcgaaag tgcgcgtttt atcgctacac gcattatcag
    11761 cgccgtaaat gactatcact ttatctggga aggacgtgta catcgggtag gtgccagtgc
    11821 cgggattacc ttgattgatg acaacaatca tcaggcggct gaagtgatgt cgcaggctga
    11881 tatcgcctgt tatgcctcca aaaatggtgg acggggccgg gtgacggttt acgaaccgca
    11941 gcaagctgcc gcacatagcg aacgggcggt gatgtcgctt gatgaacagt ggcggatgat
    12001 taaagagaat cagttgatga tgatcgccca cggtgtcgct tcgccgcgga tcccgcaagc
    12061 gcgtaatttg tggctgattt cacttaagct ctggagttgc gaaggcgaga ttattgatga
    12121 acaaacattt cgtcgtagct tcagcgatcc ggcgcttagc catgctcttg accgacgggt
    12181 attccacgat tttttccagc aggccgcaaa agcggttgcc agtaaaggct taagcatcgc
    12241 cctccccctt tccgttgccg gtttgagtag caccacactg gtaaatgaac tgcttgaaca
    12301 gctggaaaac agccctttac caccacggtt attacatctg attattccgg cagacgcgat
    12361 tttagatcac gcagaaagcg tgcaaaaact gcggctggcg ggatgtcgga tcgtattcag
    12421 tcaggtgggc cgcgatctgc aaatcttcaa ctcgctgaaa gtaaatatgg cagattacct
    12481 actacttgat ggtgagttat gcgccaacgt gcagggaaat ttgatggatg agatgctgat
    12541 tacgatcatt caggggcacg ctcagcggct cgggatgaag accatcgccg ggccagtagt
    12601 tttacccctg gtgatggata cgctttctgg catcggcgtc gatttgattt atggtgatgt
    12661 gattgccgat gcccaaccgc tggatttgct ggtgaatagc agttatttcg cgattaacta
    12721 aggcgtgctt ccccatcgcc tgatgcggcg tatcaggcct acaattcgtc cgttccgtct
    12781 ggttgccagc cttccgtata ccagatatgc aacagcgcat aagaacgcca gggcttccag
    12841 cgttcggcat aacggcggat ttgtgccggt gtcattcccg gaaatcgctg tttaatcaga
    12901 taatcatccg gcaggaaaac atctttcgcc tgccagccgc gcaaggcgaa ataattcgcc
    12961 gtccagcgcc cgatacccgg aaaagtttgc agcgttttca tcgcctgctc cacatcgccc
    13021 ggtattgtca ttggtaaggt gccctccagc gccgcatttg ccagatgaat cagcgcctct
    13081 gcccgtttca acggcatacc taacgctttt aatgcctgcg gatctgctgc tgccagccgc
    13141 tgaggcgtgg ggaagcagac ataatccgga aaatcatcca gccgttcgcc ataaagctgt
    13201 gccactctgg cggtcaattt tgccgccatc gccacgctca ccagttggcc taaaatcgcc
    13261 cgcacgccct gctcaaaagc atcaacactg ccgggtaaac gcaatccggg ccgcgccgcg
    13321 cctaacttgc ccaacgcacc gttaacaatc tgcgggttac attgcagatc aaacaggcgg
    13381 ctcattttcg ccagacactc tgcggcaaca ggttctaaac ctgcacttaa atttatgtgc
    13441 agagtatggc gggctatatc cggaatagca gtcaccacgc cgcgatattc gcccaccgcc
    13501 agactacggg cataataatc gtcagcgacc gtttccacac cgttcaccgc acgggcggcg
    13561 agaaatccca acatccacga ccagtcatat ggcggctgcc agttcagggt atacatcgca
    13621 tctccttgtt aatccgcttt cagcataaac gttattcaga cgctacgctt tgctttcata
    13681 ttccggttgt cgcgacggca acatttcgct aaagtcacgc cccttcttca ccggcatggg
    13741 gattatttcg tgtttattgg ttttgattac ggtacagcaa actgttcagt ggcggtcatg
    13801 cgcgacggta agccgcaatt actgaaaatg gaaaacgaca gcacgctgct gccttcaatg
    13861 ctttgcgcgc caacgcgtga agcggtaagc gaatggctgt accgccatca tgatgttccg
    13921 gcagacgacg atgaaacgca ggcgctgttg cgtcgggcga ttcgttataa ccgcgaagaa
    13981 gatatcgatg ttacggcgaa aagcgtgcag ttcggtcttt cctcactggc acagtacatt
    14041 gatgatccag aagaggtgtg gtttgtgaaa tcaccaaaat cattcctcgg tgccagcggc
    14101 ttaaaaccgc agcaggtagc gctgtttgag gatctggtct gcgcaatgat gttgcacatt
    14161 cgccagcagg cgcaggcgca gctgccggat gcgattactc aggcggtgat tggtcgcccg
    14221 atcaacttcc aggggctggg cggcgatgaa gcaaacaccc aggcgcaagg gattctggaa
    14281 cgcgcggcaa agcgtgccgg gtttaaggat gtggtattcc agtacgagcc ggtcgcggct
    14341 gggctggatt acgaagccac cttgcaggaa gaaaaacggg tgctggtggt ggatatcggc
    14401 ggtggtacga ctgactgttc attgctgctg atggggccgc agtggcgttc gcgtctcgat
    14461 cgtgaagcca gcctgctggg tcacagtggt tgccgtattg gcggtaacga tctggatatc
    14521 gcgctggcgt ttaaaaacct gatgccgtta ctgggcatgg gtggcgaaac cgaaaaaggc
    14581 atcgccctgc cgattctgcc gtggtggaat gcggttgcca tcaacgacgt acctgcgcaa
    14641 agtgatttcc acagtagtgc caacggtcgt ctgcttaacg atctggtacg cgatgcccgc
    14701 gaaccggaaa aagtggccct gttacagaaa gtctggcgtc agcgtttaag ctatcgcctg
    14761 gtacgtagcg cagaagagag caaaattgcg ctttcaagcg tagcggaaac ccgcgcctca
    14821 ctgccgttta tcagcgatga actggctacg ctgattagcc agcaagggct ggaaagcgcc
    14881 ctcaaccagc cactggcgcg gattctggaa caggtgcaac tggcgctgga taacgcccag
    14941 gaaaaaccgg atgttatcta tctgaccggc ggtagcgccc gttcaccgct gattaaaaaa
    15001 gcgctgacag aacagttgcc gggcattccg attgcaggtg gcgatgactt tggctccgtc
    15061 accgccgggc tggcacgctg ggcggaagtg gtgtttcgtt aattgaaaac tggctcagca
    15121 acggcttaaa tcagcctgcg cctgagccag tttttctgcc gcctgacgta gcggttccat
    15181 caacggcatg gtccgtgctg gagcatgaag gcggaatcgc tgcaactctg tcgcaccgac
    15241 agtcagcgcc gattccagcc gatgtcgttt ggcggccatt tttgccatta ccgtttgtct
    15301 gtctgcaggc gttaccgctt cgttcggcct gaaaacaaaa cggcgttcgc aactcgcttt
    15361 ccagtcgata accgcctggg tcagatgatc accaaaacct tttacttgct taacgctccg
    15421 tcgggtaacg tctgcggcgg tttcaatacc aaaagaccgt aacgccgctt tacgcgctgg
    15481 gcctacgccg ggaatagagg cagcatcgat aaaaaatccc tccagaaact tctgcttctg
    15541 ccgttccctt gcgttgtcct gaagtgctgc cagagcgcgt ttctcctctt ccggtaaccc
    15601 aagaatttcg tccttcattt tttcgagcat cgtccgtttg gcgataaaac cttccagccc
    15661 gcccaactgt tggatctggc tgactaaatg gtcatagtcc atttttgcgc gattaaaagc
    15721 ctctcttcgt cgctggactt ctgctttgta cgctttgctt gtaaggctgc caataatcca
    15781 gatagccgcc agcacgggaa ccaaaatgat ataacgcggt tctacctgaa ggccgcaaag
    15841 cagcagcgat aacgctgaca gtgcgatctc aatcagaatg atgtattcac gccgtaacag
    15901 gcctaaagga agcggcctgc cagtcggttg gaaatgatcg ggtaatggca attgcaatgc
    15961 tggcggtgct actgacgcca tcaccatcgc ccagactttc gccagcacaa aatcaccgct
    16021 ggtggtaatg acctcttcgc cgagatcaat aaaatagata acgccttgat tatccagcgc
    16081 acaccacggg cagtcggtca aatgaccggg gtaaatatgc attgccgaaa cggtacattt
    16141 ctttaactgt tggcgtagta aatcaagtgc cgctacccac gcttttgccg tcggacgccc
    16201 ggttgccacg ccactttccg tgaatgcctg ctgaaacatg gcttcaacat cacccggtaa
    16261 catcgacagc ggaatagatc gtggcggcgg tgttaaacca cgtcgctgat tatctgacgc
    16321 gtaggcataa cggaaatggg caatatccgt ctccagtgca ttccccgcat cagagataag
    16381 cggcacacca gaataaggat gccgcccacc aaacaagacg tgaaaaatca gcaacgcaag
    16441 gccaaaatta tcgtgatttt ctgtgcgttc aaagccgata aatgacgaca aggtttgtag
    16501 ctctggcggc gtaaaatgcg acacgccgac ttcgcataaa tgcagcgtgc cattggcgtt
    16561 aatctgaaag gagtcgctat caatcaacac cactttgctg tcgcgaccta ccataaagct
    16621 gttctggttt acgtcaccca cgacgtgccc gtgctcgtga atcgtagcaa aagatgaagc
    16681 aatattgcgc gcaacataga gtagaaaatc ccacgcacaa tgagggtaac tctggcgacg
    16741 atgtgccggg ctatagatca tatgaatcgg ttctttacca gaaacttttg gcatcataaa
    16801 accgataacc tttccgcctc gtccaccgtg aagcgttgcc tgcggccagg cgacataatt
    16861 caacaattgc gcgtcagctg tcgcagccat aaaggcaagt ttgtcctgtt ttaaggcggg
    16921 tggcggcgtg tgataaatct tggcgacgct atcgacaaac tcctcgatat cataaaccgc
    16981 accttcgccg cctttgccca gttcacggcc aggcgtaacg cactcaccag tagcagtata
    17041 taaagtgggt ttcatggtaa ccacagcgcc agtgccaacg ttttatcatc atcggtacgc
    17101 tcgtttaccg ccgggctgga caaaaactgc tttaacaatt caggtaataa atcgagttgt
    17161 tcctgcgttg ctgacgccag tccattgaaa aacggggtaa agaaaggcac atgcggagaa
    17221 ttatccagca tatttaacgc cagtcgctga ataccatccg taaatgctgc aactttatgc
    17281 gcacgctcag tactggtgta agtttccagt ctggaaacag catcgtcatc ggtaatgaag
    17341 tgcgtcatat tggcatattc ccccaccatc ggcgtgagcg gcagttgcaa accgtgaccc
    17401 aaatctacca ccacgccgcc atcgccaatc tgcataatca aggtgccatt agctgatgaa
    17461 atcaatccca aaaacgtgca ggcaaaatcg cgaaccgcta attctttggc ttccgcctcg
    17521 gcaaaaagtc gctgacgaat ggtcagcaca atgtccgttg cgaggacatc attaagcccc
    17581 aattccccgc cctgcacttt ttgcgacata taagccatcg cttcattgac ggcgagcatc
    17641 gcaccttcgc caccctgcga gacgctgcct gcgccatcag cgacaaatac cgacaataat
    17701 ggctgctggt cttctaacca ggcaacctgc atctggcagg catcctggca agacaagtca
    17761 gcgctgatgt gcgacgttcc aacggctgat gcataaacca gacgccagct cacactgacg
    17821 tccagccttt tggcgcttcc agcacaactt ccgttcccgg cgtggagcgg gaaaccgaac
    17881 gcagcgaact ggataaccag ctaaatagtt cgcggaattg taatccttga agaggcaaag
    17941 gctgacgaac gctgatttgg gccagcgtct tcatatccgc cccctgaaca ccgatggaaa
    18001 agaaggcaaa cttcttatct tcttccccct gaaacacctt gttggcagca gcctgccact
    18061 catcggttgg tgcgccatcg gtaatcagga aaatccacgg acgataatag gaaataccat
    18121 tggcgcgata ctcacgtttg cgctcctcaa ccatattcag ggctttggta atggcggcgc
    18181 ccattggtgt atccccatgg gcaaacagga tgggcgggaa aaaattagcg gcactggtaa
    18241 atggttgttc cacatgcacc gggccgaacg tcacaatacc gagttccact ctttttaagg
    18301 ccagcgaatc ggcaagcagc tcatcgcgaa aggtaaccaa tccggcgtta agttcattaa
    18361 taggtctacc gttcatagaa ccagaaacat ccagcagcaa aatgcagggg caacgtggtt
    18421 ccgggttact ggcaaaatcg ctggtggcaa acgtgatttg ttcgctcata agaaatcctt
    18481 tcttagagaa ttattgaggt ccggtatcat accgattgca ataatgaaat aagagcaaac
    18541 gaagaaactt tcagaattag gcccggcggc gcagaaaatt ttgctattcg cctcgcaaga
    18601 acggagagcc gtcgattgac actaagggcg gagtgacata atttcaggag tgagggttag
    18661 ggagaggttt cccccctccc cctggtgttc ttagtaagcc tggaagctaa tcactaagag
    18721 tatcaccagt atgatgacgt gcttcatcat aaccctttcc ttattaaaag ccctcttctc
    18781 cgggagaggg tttcccgttt cagcgtcccg ctgaaatcgt cggcttacct cctttcgccc
    18841 tgaatgcagt ctatcgctaa cgcgtgaaca aaatagtttc ctctgtgatt attgcgtaac
    18901 gactcgcaaa gaatatttgc tggcaggatc gcagactaca aagcctgcgg attgacaatc
    18961 ttatcgtgaa ggcataattt caggagtgag ggtagagcgg ggtttccccc gccctggtag
    19021 tcttagtaag ctggccagct aataactaag agcaccacga tgatgagtag cttcatcatg
    19081 accctttcct tatttaaggc cccttcctcg ggaggggctt tcccgtttca gcgtcccgct
    19141 gaaatcggcg gcctacctcc tttcgccatg caagcagtct atcgctaact tgcagataaa
    19201 atagtttcac atgtgattat tgcgtaacgt ctcgcaaaga atatttgctg gcaggatcgc
    19261 agactacaaa gcctgcggat tgacaatctg attgtgaagg catactttca ggagtgaggg
    19321 tagagcgggg tttcccccgc cctggtagtc ttagtaagcc gggaagctta tgactaagag
    19381 caccacgatg atgatccact tcatcatgac cctttcctta tttaaggcct cttcctcggg
    19441 aggggctttc ccgtttcagc gtcccgctga aatcatcggc ttacctcctt tcgccctgaa
    19501 tgcagtctat cgctgacttg cggataaaat agtttcacat gtgattattg cgtgacgtct
    19561 cgcaaagaat atttgctggc aggatcgcag actgcaaagc ctgcggattg acaatcggac
    19621 cgtgaaggca tactttcagg agtgagggta gagcggggtt tcccccgccc tggtagtctt
    19681 agtaagctgg gtagctaacg actaagagca ccacgatgat gagtatcttc atcatgaccc
    19741 tttccttatt taaggcccct tcctcgggag gggctttccc gtttcagcgt cccgctgaaa
    19801 tcgtcggcct acctcctttt gccctgaatg cagtctatcg ctaacgcgta gataaaatag
    19861 tttcctgtgt tattactgga tgggtgctcg caaatgtgcc cgccattcag acgattcccg
    19921 atagtgtttc ataattcctc catttttctc cctgattggc tggctacact agtatcattc
    19981 cgcgaaacgt ttcaggaaga gaaactctta acgatgaaag gcagttataa atcccgttgg
    20041 gtaatggtaa tcgtggtggt tatcgccgcc atcgccgcat tctggttctg gcaaggccgc
    20101 aatgactccc ggagtgcagc gccaggggcg acgaaacaag cgcagcaatc gccagcgggt
    20161 ggtcgccgtg gtatgcgttc cggcccatta gccccggttc aggcggcgac cgccgtagaa
    20221 caggcagttc cgcgttacct caccgggctt ggcaccatta ccgccgctaa tactgttacg
    20281 gtgcgcagcc gcgtggacgg tcaactgatg gcgttgcatt tccaggaagg ccagcaggtc
    20341 aaagcaggcg atttactggc agaaattgac cccagccagt tcaaagttgc attagcacaa
    20401 gcccagggcc aactggcaaa agataaagcc acgcttgcca acgcccgccg tgacctggcg
    20461 cgttttcaac aactggtaaa aaccaatctc gtatctcgtc aggagctgga tgcccaacag
    20521 gcgctggtca gtgaaaccga aggcaccatt aaggctgatg aagcaagcgt cgccagcgcg
    20581 cagctgcaac tcgactggag ccgcatcacc gcaccagtcg atggtcgcgt tggtctcaag
    20641 caggttgatg ttggtaacca aatctccagt ggtgatacca ccgggattgt ggtgatcacc
    20701 cagacgcatc ctatcgattt gctctttacc ctgccggaaa gcgatatcgc taccgtagtg
    20761 caggcgcaaa aagccggaaa accgctggtt gttgaagcct gggatcgcac caattcaaag
    20821 aaattaagtg aaggcacgct gttaagtctc gataaccaaa tcgatgccac taccggtacg
    20881 attaaagtga aagcacgctt caataatcag gatgatgcgc tgtttcccaa tcagtttgtt
    20941 aacgcgcgca tgttagtcga cacagagcaa aacgctgtgg tgatcccaac agccgccctg
    21001 caaatgggca atgaaggcca ttttgtctgg gtgctgaata gcgaaaacaa ggtcagcaaa
    21061 catctggtga cgccgggcat tcaggacagt cagaaagtgg tgatccgcgc aggtatttct
    21121 gcgggcgacc gcgtagtgac agacggcatt gatcgcctga ccgaaggggc gaaagtggaa
    21181 gtagtggaag cccagagcgc caccactccg gaagagaaag ccaccagccg cgaatacgcg
    21241 aaaaaaggag cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc
    21301 gcctgtttat tatgcgtcct gtcgccacca cgctgctgat ggtggcgatc ttactcgccg
    21361 ggattatcgg ttatcgagcc ctgcccgttt cggcgctgcc ggaagtggac tatccgacca
    21421 ttcaggtagt cacgctctac ccaggtgcga gcccggatct catgacctct gccgttaccg
    21481 cgccgcttga acgccagttt gggcagatgt ctggcctgaa acagatgtcg tcgcaaagtt
    21541 ccggcggtgc gtcagttatc actttgcagt tccagctaac attaccgctc gatgtcgccg
    21601 agcaggaagt gcaggccgcg attaacgctg cgaccaactt gttgccgagc gatctgccta
    21661 acccgccggt ttacagcaaa gtgaacccgg cagatccgcc gatcatgacg ctcgctgtca
    21721 cctcaaccgc catgccgatg acgcaagtgg aagatatggt ggaaacccgc gtcgcgcaga
    21781 aaatctcgca gatttccggc gtcggcctgg tgacactttc cggcggccag cgtccggctg
    21841 ttcgtgtcaa acttaacgct caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc
    21901 gcaccgccat taccggcgct aacgtcaact cggcaaaagg tagcctcgac ggcccttctc
    21961 gtgcggttac gctttccgcg aacgaccaga tgcaatccgc cgaagagtat cgccagctaa
    22021 tcatcgccta ccagaacggc gcaccgattc gtctgggcga tgtcgcaacc gtagagcaag
    22081 gtgcagaaaa cagctggctt ggcgcatggg ctaacaaaga acaggccatt gtgatgaatg
    22141 ttcagcgcca gcccggtgct aacattatct ccaccgccga cagcatccgg cagatgctgc
    22201 cacagctcac cgagagtctg ccgaaatcgg tgaaggtgac agtactttct gaccgtacca
    22261 ccaatattcg cgcatccgtg gatgatactc agtttgaatt gatgatggct atcgcgctgg
    22321 tagtcatgat tatctacctg tttttgcgta atattccggc gaccatcatt cccggcgtcg
    22381 cagtgccgct gtcgttaatc ggcaccttcg cggtcatggt gtttctcgat ttttcaatca
    22441 ataacctgac gctgatggcg ttaactatcg ccaccggatt tgtggtcgat gacgccatcg
    22501 tggtgatcga aaacatttcc cgctatatcg aaaaaggcga aaaaccgctg gcggcggcgc
    22561 tcaagggcgc aggtgaaatc ggctttacca ttatctcgtt gaccttctca ctgattgcag
    22621 tgctgatccc gctactgttt atgggcgata tcgtcgggcg actgttccgc gagtttgcca
    22681 ttaccctggc ggtagcaatt ttgatctcag ccgtggtgtc gctgactctg acgccgatga
    22741 tgtgcgcgtg gatgctcagc caggagtcgc tgcgtaaaca gaaccgcttc tcccgtgcct
    22801 cggaaaaaat gttcgacagg ataatcgccg cctatggtcg tggactggcg aaagtgctga
    22861 atcatccgtg gctgacctta agcgtggcac tcagcacgct gctgcttagt gttctcttgt
    22921 gggtgttcat tccgaaaggt ttcttcccgg tacaggacaa cggcattatt cagggcactt
    22981 tgcaggcacc gcaatccagc tcctttgcca atatggccca gcggcaacgc caggtcgcgg
    23041 atgtgatttt gcaggatccg gcggtgcaaa gtctgacctc atttgttggc gttgatggca
    23101 ctaacccgtc gctgaacagt gcgcgtttgc agatcaacct caaaccgttg gatgaacgtg
    23161 atgatcgggt gcaaaaagtc atcgcccgtc tgcaaacggc ggtggataaa gtgccgggcg
    23221 tcgatctctt cctgcaacca acgcaggacc tgaccatcga tactcaggtc agccgcaccc
    23281 agtaccagtt taccttgcag gccacgtcac tggatgcgct cagtacctgg gtgccacagt
    23341 tgatggaaaa actccagcag ttgccacagc tttctgatgt ctccagcgac tggcaggacc
    23401 aagggctggt ggcgtatgtc aatgttgatc gcgacagcgc cagccgtctg gggatcagca
    23461 tggcggacgt cgataacgcc ctttacaacg cgtttggtca gcgactgatt tccactattt
    23521 atactcaggc caaccagtat cgcgtggtgc tggagcacaa caccgaaaat actcctgggc
    23581 ttgcggcgct ggatactatt cgcctgacca gcagcgacgg cggcgtggta ccgctaagct
    23641 caattgccaa aattgagcag cgttttgcgc cgctttccat caaccatctg gatcagttcc
    23701 cggtaacgac catctccttt aacgtgccgg ataactattc gctgggcgat gcggtgcagg
    23761 cgattatgga caccgaaaag acgctgaatc tgccggtgga tatcaccacg cagttccagg
    23821 gcagcaccct cgccttccag tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg
    23881 tggtggcgat gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca
    23941 ttctctcgac gctacccacg gcaggtgttg gcgccctgct ggcgttaatg attgccggta
    24001 gcgaactgga tgtgattgcg attatcggca ttattttgct gatcggtatc gtgaagaaga
    24061 acgccatcat gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc
    24121 gcgatgccat ctaccaggct tgtctgttgc gttttcgtcc gatcctgatg accaccctgg
    24181 cggctctgct tggcgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc
    24241 gtccgttagg tatcggcatg gtcggcggcc tgattgtcag ccaggtgctg acgctgttca
    24301 ccacgccggt gatttatctg ctgtttgacc gcctggcatt gtgggccaaa agtcgctttg
    24361 ctcgccatga agaggaggcg taagtgaagt tttttgccct cttcatttac cgcccggtgg
    24421 cgacgatttt actgtcggtc gcaattaccc tgtgcggcat actgggtttc cgtatgctgc
    24481 cggtcgcccc gctgccgcag gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg
    24541 gtgcgtcgcc ggagacaatg gcgtcttccg ttgccacgcc gctggagcgc tcacttgggc
    24601 gcattgccgg ggtcagtgaa atgacctcca gcagttcgct cggcagcacg cgtattattt
    24661 tgcagtttga ttttgaccgg gatatcaacg gtgcggcgcg tgatgtgcag gcggcgatca
    24721 acgccgcaca aagtctgctg cccagtggga tgcccagccg cccgacttat cgcaaagcga
    24781 acccgtcgga tgcgccaatt atgatcctga cgctaacctc cgatacttat tcgcagggtg
    24841 aattgtacga tttcgcctcg acgcagctgg ctccgacgat ttcgcaaatc gacggcgtcg
    24901 gtgatgtcga tgtcggcggc agctcgctgc ccgccgtgcg cgtcgggctg aacccgcagg
    24961 cgctgtttaa tcagggcgtg tcgctggacg acgtacgcac cgccatcagc aatgccaacg
    25021 tgcgtaaacc gcagggggcg ctggaagatg gcactcaccg ctggcagatc cagaccaacg
    25081 atgaactgaa aaccgccgct gaatatcagc cgctgattat tcattacaac aacggcggcg
    25141 cggttcgtct gggcgatgtg gcgacggtga ccgactcggt gcaggatgtg cgcaacgccg
    25201 ggatgaccaa cgccaaaccg gctattttgc tgatgatccg caagctgccg gaagccaata
    25261 ttatccagac ggtagacagc atccgggcaa aattaccgga gttgcaggaa accattccgg
    25321 cggcgattga tctgcaaatt gctcaggatc gctcccccac cattcgcgcc tcgctggaag
    25381 aagtcgagca aacgctgatt atctcggtgg cgctggtgat tctggtggtg tttttattcc
    25441 tgcgctcggg tcgcgcaact attattcccg ccgttgcggt gccggtttcg ctgattggta
    25501 cgtttgcggc gatgtacctg tgcggtttca gtctcaataa tctttcgtta atggcgctca
    25561 ccatcgctac tggtttcgtg gtggatgacg ccatcgtggt gctggaaaac attgcccgtc
    25621 atctggaagc gggaatgaaa ccgttgcaag ccgcactgca aggtactcgc gaagtcggtt
    25681 ttacggtgct gtcgatgagt ctgtcactgg tggcggtgtt cctgccgctg ctgttgatgg
    25741 gcggattgcc gggccgactg ttacgcgaat ttgccgtgac gctttctgtc gccattggta
    25801 tatcgttgct ggtttctctg acattaacgc caatgatgtg tggctggatg ctgaaagcca
    25861 gcaagccgcg cgagcaaaag cgactgcgtg gttttggtcg catgttggta gccctgcaac
    25921 aaggctacgg caagtcactg aaatgggtgc tcaatcatac ccgtctggtg ggcgtggtgc
    25981 tgcttggcac cattgcgctg aatatctggc tgtatatctc gatcccgaaa accttcttcc
    26041 cggagcagga cactggcgtg ttgatgggcg ggattcaggc ggatcagagt atttcgtttc
    26101 aggcgatgcg cggtaagttg caggatttca tgaaaattat ccgtgacgat ccggcagtgg
    26161 ataatgtcac cggctttaca ggcggttcgc gagtgaacag cgggatgatg tttatcaccc
    26221 tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt ctgcgcgtaa
    26281 aactggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaggat attcgcgttg
    26341 gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac ctggcggcac
    26401 tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa ctggcggacg
    26461 tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac cgtgacacca
    26521 tggcacggct gggaatcgac gtacaagccg ccaacagtct gttaaataac gccttcggtc
    26581 agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg atggaagtgg
    26641 atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc aataacgaag
    26701 gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc ccactatcgg
    26761 tgaatcatca gggattatcg gcggcctcga ccatttcgtt taacctgccg accggaaaat
    26821 cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggg gtgccttcga
    26881 cggtgcgcgg cagttttgcc ggcacggcgc aggtgttcca ggagacgatg aactcgcagg
    26941 tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga
    27001 gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt ggagcgctgc
    27061 tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg atcatgctat
    27121 taatcggcat cgtaaagaaa aacgccatca tgatggtcga ttttgcgctt gaagcccaac
    27181 ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc
    27241 cgattatgat gactaccctg gcggcgctgt ttggcgcgct gccgctggtg ttgtcgggcg
    27301 gtgacggctc ggagctgcgc caacccttgg ggatcaccat tgtcggcggt ctggtaatga
    27361 gccagcttct gacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctgcggc
    27421 tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat cttcccgaca
    27481 gcacccgctg gcaattgtgg attgtggctt tcggcttctt tatgcagtcg ctggacacca
    27541 ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata
    27601 tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc gccagcggct
    27661 ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg ctgtttactc
    27721 tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt
    27781 tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg atgaaaatcg
    27841 taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt caggtcggtc
    27901 cgctgctcgg tccggcgctc ggcggtctgc tggtggagta cgcgtcgtgg cactggatct
    27961 ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgctg ttaatgccga
    28021 actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg gcggttggca
    28081 tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg ccgctggcga
    28141 ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg cacgccagaa
    28201 ataacaaccg tgccctgttc agtctgaaac tgttccgtac tcgtaccttt tcgctgggcc
    28261 tggcggggag ctttgccgga cgtatcggta gcggcatgtt gccctttatg acgccggttt
    28321 tcctgcaaat tggccttggc ttctcaccgt ttcatgccgg actgatgatg atcccgatgg
    28381 tgcttggcag catggggatg aagcggattg ttgtacaggt ggtgaatcgc tttggttatc
    28441 gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg tttatgacta
    28501 ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa ggaatggtca
    28561 actcaacgcg tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaatctgg
    28621 cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca
    28681 ctatcgccgg gctgttgctg ggactttttg gttcgcagca tgtcagcgtc gacagcggca
    28741 ccacacaaac cgtctttatg tacacctggc ttagcatggc gttgatcatc gcccttccgg
    28801 cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa
    28861 aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt ttctggcgat
    28921 tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta tcagttttga
    28981 gcgtggcttt attgattaca tcaagcatgg taatgaacaa cgattacaac tgttaagtga
    29041 tgcgcttggc gagcagtatg cgcagcatgg caactggcgc ttcctgcgca acaatgatcg
    29101 ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata aacccggccc
    29161 gggtatgcca ccgcacggct ggcgtaccca gttctgggtg gttgatcaaa acaacaaagt
    29221 gctggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgatcca ttctggtcaa
    29281 cggtgcggaa gttggcgcgg tgatcgcctc ccccgttgag cggttaacgc gcaatactga
    29341 tatcaatttc gataaacaac agcggcaaac cagctggttg attgtcgctc tggcaacgtt
    29401 actcgcggca ctcgctactt ttctgctggc gcgcggtttg ctggcaccgg taaaacgact
    29461 tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa cgcccaccag
    29521 tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag ctcgccagct cgctggagaa
    29581 aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc gtacgccatt
    29641 agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat tcacgccgga
    29701 gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg acgatcttca
    29761 tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg tagatttgat
    29821 cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga tttgccagcc gtggactgaa
    29881 actgcaattt tccctgccag acagtatcac cgtatttggc gatcgcgacc gtttaatgca
    29941 gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg gcagcctgaa
    30001 aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg
    30061 tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg aaggttcccg
    30121 caaccgcgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc
    30181 acataatggc cgcattattg ctgcccattc gccttttggc ggggtaagca ttacagtaga
    30241 gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac
    30301 acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg gacagttgct cattgattat
    30361 ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt actgccgtat
    30421 gtgcgccaga caccaccgga tctgatcctg ttagatctga tgctccctgg caccgatggc
    30481 ctgacgctgt gccgggaaat tcgtcgtttt tctgacattc cgatcgtgat ggtgacggca
    30541 aaaatcgaag agatcgatcg cctgctgggg ctggagattg gcgcagatga ttatatctgt
    30601 aagccgtaca gcccacggga agtggtagcg cgcgtcaaaa ccattttgcg ccgttgcaaa
    30661 ccgcagcgcg agttgcagca acaggatgct gaaagcccgt tgattatcga cgaaggtcgt
    30721 tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt tcgtctgctg
    30781 aaaacgctct ctcatgaacc aggaaaagtg ttctcccgcg agcatttgct caatcatctt
    30841 tatgacgact accgcgtagt aaccgaccgc accatcgaca gccacattaa aaacctgcgc
    30901 cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta cggcgtcggt
    30961 taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta ttttgttagc
    31021 cggagatggc gttcagaccc gatccggcaa cattatccca cgcatggtca gcaaactgac
    31081 attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatcg gtaaacagca
    31141 cctttagatg ctgttttcga tacacaattt caatcaagga gtcattatgg ctggttggtt
    31201 tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg gcaatggtga
    31261 gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg gcatcgcgtc
    31321 ggtgcgtagc aacagcccgc aagaagaacg ctatgagaaa aaaacggcaa gtaacggcaa
    31381 attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc agatgtacgc
    31441 caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca caagccagac
    31501 ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc ggctttattt
    31561 tctctcatca cagagttact atccctcaag aaattattaa ccatgaaatg agttttctcc
    31621 tcataactga taaggaaaat tatgctcaaa ggattgcgta cattattttc acgcagggtt
    31681 ttgtttaacg tcgaaatgaa taattctgtt gactgtgaat tacttctcat ccagcaacaa
    31741 ctccaggcat tgatagccga accaggcaat tatttagcac atgattgtga tctcaacgat
    31801 actctggttc tttgccagct tacccatcat cacaatggat atatacgaga aaaggccgtg
    31861 cagtatctgg ggcacaaaga ggatgttgcg gctatagaag aattattact tgcagctaat
    31921 gactgggtaa aagaggttcg ccttgctgca accatagcat tacgtcattt aatgaaggat
    31981 aagaatgcag cggcatttgc cgttaatcta cccatgatac gtaaccttct ccaatgccag
    32041 cgttacgatc accgtgcatt tgtagacgag atacttagat atttattgac agaaaataac
    32101 cgcccacaat taacgtattg gcttacttgc cctgataaaa agctctcccg cgcggtatta
    32161 caaacgctga ttgaatacgg ttactttaat gatgaatctt cactgctttc aatactcgat
    32221 aaacccgatg agggtttgcg aatgctggcg accacccact ggctgcgtca acaattacca
    32281 ttaagcgaag cggtattaac tcgtctgtta aaagaccgct ggccgcgtat tcgacaagca
    32341 acgttattta gcctcactga ccgagcaata gaaatgcctc cggcgttgca cagcacatta
    32401 ctgctggata acaatatgtt gatccgccta cgcgctaaaa atatgcttca cgaagtcatg
    32461 gatgttccgc aattctggcg tcatgttgtc acttctgcgg aatatactcc ctcacagcga
    32521 cgggccgctt tatacgggct ggattccatt catgatccca atattttaaa actggcagaa
    32581 tggggattat cacagaatgt attcccactg cgtttagccg ctatgcacat tcttgcaaaa
    32641 gctaatcctc gttgtggcgt caaagagact attctgacca cgctggctaa cccggacgcc
    32701 gccgggttgc gatttatggt taacatctgt gtatggtgtc gcgttccgct cacgttcgag
    32761 gaaatacgtc agttgcagga aaacgcgccg tcagttaaac atgcttgcgc atattgtcgg
    32821 ctgtatcata acttgaacaa atgggacggt ttaattcttc ttcttcaatc gcaacacaag
    32881 ctaactgaag aatttgcagg taaacagctc gcgatatggc aacgcaattt caatctttcg
    32941 ggcattcaac cgaatgctct tcagcgtcag caacttcagg cgctttttac ccgcaatccg
    33001 gaacttcaca acagattatg gggttacatt ccttttaaat aacttcgaat tatagactta
    33061 cgatgacaaa tgtacctgct gacgagcgga accaaaacga aacaatacgt gaagctttac
    33121 gcttgctacg agaaaaactt gccgagggac tacgcagtgg cgaagcccaa ccgtgggaaa
    33181 aggatctgtt tttacacaaa gtgaaggccg gtaacaaata aatcccccag tcttttccct
    33241 cacatttgca ctggagccaa ccccgccgat tgcgctacaa tgcccgccct taaagtgggg
    33301 gcactcccct aaccgcttca tcaggtgaag cggatctgac ctgtcatcag aacgagagaa
    33361 ttatgtttaa accggaactc ctttccccgg cgggaacgct gaaaaatatg cgttacgctt
    33421 tcgcttatgg cgcagatgct gtttatgcgg gccagccgcg ttactccctg cgtgtgcgca
    33481 acaacgaatt taaccacgaa aatcttcagc tcggcatcaa tgaagcccac gcgctgggga
    33541 aaaagtttta tgtcgtggtc aacattgcac cacacaacgc caagctgaaa acctttatcc
    33601 gtgacctgaa accggtggtg gaaatggggc cggatgcgct gattatgtcc gatccagggc
    33661 tgattatgct ggtgcgtgag cacttccctg aaatgccgat ccacctctcg gtgcaggcta
    33721 acgccgtaaa ctgggcgacg gtgaaattct ggcagcaaat gggcctgacc cgcgtgatcc
    33781 tctctcgcga gctgtcgctg gaagagattg aagagatccg caatcaggtg ccggatatgg
    33841 agatcgagat cttcgttcac ggtgcgctgt gcatggccta ctccggtcgc tgcctgctct
    33901 ctggctatat caacaagcgc gatccgaacc agggtacctg caccaacgcc tgccgctggg
    33961 agtacaacgt ccaggaaggg aaagaagatg acgttggcaa catcgtacac aagtacgagc
    34021 cgattccggt gcaaaatgtt gagccgacgc taggtatcgg cgcgccaacc gacaaagtgt
    34081 ttatgatcga agaagcccag cgtccgggcg agtatatgac cgcgtttgaa gatgagcacg
    34141 gcacttacat catgaactcg aaagatctgc gcgccatcgc ccatgtagaa cgcctgacca
    34201 aaatgggcgt gcattcgctg aaaatcgaag gccgtactaa atctttctac tattgcgcac
    34261 gcaccgcaca ggtttatcgt aaagctatcg atgatgccgc tgcgggcaaa ccgttcgata
    34321 ccagcctgct ggaaactctg gaaggtctgg cgcatcgtgg ctataccgaa ggtttcctgc
    34381 gtcgtcatac tcacgacgat tatcagaact acgaatacgg ttattcagtt tctgaccgcc
    34441 agcagtttgt tggtgagttt accggtgagc gcaaggggga cctcgcagcg gtagcggtga
    34501 aaaataaatt ctccgttggc gacagccttg agctgatggc gccgcaaggc aacattaact
    34561 ttacccttga gcacatggaa aacgccaaag gtgaagcgat gccggtcgca ccaggcgatg
    34621 gttatactgt gtggctcccg gtcccgcagg atcttgagct aaattacgcg ctgctgatgc
    34681 gtaatttctc cggggaaacc acgcgtaacc cacacggtaa gtgattaatt tcgattattt
    34741 ttcccggatg gaaaattctt agaaaccgat cacatacagc tgcatttatt aaggttatca
    34801 tccgtttcgc tgaaaaacat aacccataaa atgctagctg taccaggaac cacctcctta
    34861 gcctgtgtaa tctcccttac acgggcttat tttttatgca taagccctat ctctggtaac
    34921 cgtcttccat tgaccacatc gatagaatcc tccttcatag cacgatgcct ttcacttatc
    34981 ggcatcgtgc tcccacaggt tccggctacg cacagccaga acgcgcatat ttgacgctta
    35041 ccaaaaaata ttctcactct ccacatttga atgtcagacg agcgacgcca tgtaatcctg
    35101 caccttctgt cttcaggtca actatctgca tttttttgcc ctgagtaaca cagaaatggg
    35161 ctgcatcatt ttttactata ttttctgcac cagatattct gcccctggct aaagaagctt
    35221 cggcttcggt gtagtattgg ttatcgagtt tacgctgaat attactttta tatgcaagac
    35281 caaatttacc gatacttgtc tcatcattat gcacagcaca accagacata ataaaaatac
    35341 taattaatga tatagcagct atctttttca tctcaccttt ccccattaaa taccaacgac
    35401 actctctagt gtttaaatat aataatggct gattattata tttgaatagg attataataa
    35461 atgttctgta caacatttcc tacataagta ggaattacag acatgaaggc cctttccagg
    35521 taactccatg gggctttaat atatttggaa tagtatattg aagatgccac tgtttggttg
    35581 aatattaggc atatgctctt ttttgaaatt tatcggtggc taccgttaat attcgctgtt
    35641 cccattgcaa gctcctggtg gtaaccactg aatcctccat acttgaactg acttttcatc
    35701 ctccgacttt catcctgttc tgactccacc ttttcttttc tgctctacac tatctacaga
    35761 ccaatcataa aggcacatac gatcatggca gaatttcccg ccagcttact gattcttaat
    35821 ggcaaaagta ctgacaatct acccttgcgc gaagcaatta tgctgttgcg tgaggaagga
    35881 atgacgatcc atgtgcgggt cacctgggag aaaggcgatg ccgcacgata tgtagaggag
    35941 gcccggaagt tgggcgtcgc aacggtgatt gccggaggtg gcgatggcac cattaatgaa
    36001 gtttctacgg cgttgattca gtgtgagggg gatgacatac ccgcgctggg aattttgcca
    36061 ttaggaaccg ccaatgattt tgccaccagt gtagggattc ctgaggcact ggataaggcg
    36121 ctgaaactgg caattgccgg taacgccatt gcgatagata tggcgcaggt caacaaacaa
    36181 acctgtttta ttaatatggc gacaggcgga tttgggacgc gtattaccac agaaacgccg
    36241 gaaaaattaa aagccgcgct gggtggcgtc tcttacatca ttcatggctt aatgcgcatg
    36301 gatactctgc aaccggaccg ttgtgaaatc cgcggtgaaa actttcactg gcaaggtgac
    36361 gccctggtca ttggtattgg taacgggcgt caggccggtg gcggtcaaca attgtgcccg
    36421 aacgcgttaa ttaacgatgg cttgctgcaa ctgcgcattt ttaccggcga tgaaattctt
    36481 ccggctctcg tatcaacctt aaaatctgac gaagataacc cgaatattat cgaaggcgct
    36541 tcgtcgtggt ttgatataca agccccacac gaaatcactt ttaatcttga tggcgaaccg
    36601 ttgagtggac aaaactttca tattgaaata cttccggcgg cgttgcgttg tcgattacca
    36661 ccagattgtc cattattgcg ttaatcaaac tattctctct tgaaccaggg agaatccgta
    36721 atgccggatg cggtgtaaat accttatccg gcatacagaa caatactaca ccgtaattaa
    36781 tttcacccgt gtcttctgca atgccagttt atccacctca gacacagcat catcggtaat
    36841 aataatgtca attttttcga ttggcagtac ctgattaaaa ccgcgacggt taaatttcga
    36901 tgaatcgaga acggcaatca ctttattagc ggctgtgacc atcgcaccgc taacggaata
    36961 accctcatta aaggtcgtaa taccattaac cgcatcaata ccatcagcac cgacaaacat
    37021 taaatcggca ttaatatcct gcagggaacg ttcagcaata gatccatgca tagatcgcgt
    37081 tttatggcgg accgtgccac cgcagacgac cagagtaatg tctttatttt cggaaagggc
    37141 aaacgcagcc gggagactgt tggtaatcac cgtgatattt ttggcggtca ttaatccttc
    37201 ggcgataagc attgtggtac ttccgctgtc gagaataacg gtcatccctt catggatcat
    37261 tttgactgcc gcctgcgcta ttctgttttt cggcgcgctg gcgagctgaa atcgctcttt
    37321 aaacccgact tcctgggtct cggtttcact attaccggac attattttcg ccgcaccgcc
    37381 atgaaagcgc gtaaccacgc ctttttgttc gagaaagcgc aaatcggcac ggattgtcgc
    37441 ttccgaggca gcaaatactc ccgccagatc ctgaacaagc acggttccct gttcattcac
    37501 taattggatg atcttattcc ttcgctcgaa tgaattcatc gtcatttacc catattcaat
    37561 tgtggctagt gtaaacgaag ggggaccatt ggtgaatgat ctgttttgca caataccggg
    37621 ccagcatcac gctggcccgc ggtttcaggg aatgagcaac actttgcccg gcatggcatt
    37681 acgagcgatg tcacgcaccg cctgggcgaa gctttcaaag cttccacggt gagcgattaa
    37741 tggctccagg cttaacttac gttctgtcag caaccggctc gccgtttccc actcctgccc
    37801 cggccaaggg ctggagtagt tcatccaact gccgataacc gtcagctctt tacgcaatat
    37861 tttgccaaac gttgccgatg ttaaatgcag atcctgatgc aacgtgccca ccagcgccag
    37921 ttgggcatga ggaccggcaa tctctaccgc cagttcgaca gtttgcggta cgccagccgt
    37981 ctcgaggata agctgattaa agcgcagttc gcgtaaaacg ctctgcattt gcggcgcgct
    38041 catttcgcta ctgttaaatg tttgcatcgc accgaaagat tttgccagtg ccagtttttc
    38101 tgagctaatg tcgatagccg tcacactctt tgctcccagc gcgacagcgc actgaatagc
    38161 cagcaggcca atggttccgg caccaataat aataacgttt ttattctcac aaccttgcgc
    38221 taaatgaaaa gcatgcagtc caacggtaat cggctcaata aaagccccat cctcaatagg
    38281 catatccgtg ggtagagcaa agacattttt tcgcttaacg acaatatatt cagcaaatcc
    38341 accatcacgc cgcgagccaa taaaatcata ttttgcgcac tgggaataaa accctttcaa
    38401 acactctgga caagtaaaac agggtaataa cggcacactg gcaaccgcat cgccagggtg
    38461 taaatcatca acaccggatc ccaccgcatc aatatagccg ctaaattcat ggcctaacgt
    38521 tattggataa taatgtgcac catttttaaa tatcctgggt aaatcggaac cacataagcc
    38581 cgagctggca atttttaccc gtacctcatc ctgatgttta atttcaggaa tgacgctttc
    38641 tgcaacgcgc acgataccat cagtatcatt caccactgat ttcataaaaa ctcctgattg
    38701 ttaaggggga taaactcccc ctcagttaaa aattattctg cgagaacggc tttctcttgt
    38761 ttaataaaac cacgcgctct acgccaggtc atgaaaatac cggtcagata aattgcgccg
    38821 ataataatga aaccgggaat attttgcggg gagaaaacct gaatcagtaa ccaggtaatg
    38881 ggagaaccgc cctgatccat tgaagccacc atacccccgg cttttaacgc cccagcatta
    38941 gccgccagtt gggtgtgcaa accaatagtt tgcgtcgcga tccacagagt gatgctcatg
    39001 atgatgacac ccgagattaa ggtgcggaac agatttccac gatgcacggc gactgccatc
    39061 gccacaaaga agccgatggt ggcaagatcg ccaaacggca gtacctgatt acccggaaca
    39121 caaacagcaa ttaaaatggt gagcgggata aaaatcaggc ttgccgatac caccgccgta
    39181 tgccccagca gtaatgcggg atccaggcca atcaggaact cctgaccgcc gaacttcgcc
    39241 tgtaaacgac tacgggcctg cttagcgatg ggcgttaaac catccatgat gggtttaatc
    39301 acccgtggca ttagcagcat cactgccgcc gtttttaccg ccagctgcaa tacacctttg
    39361 acatcgtaac ccgcgaggat gccgataatc agccccatca caaaacccac ggtgacaggt
    39421 tcgccaaaag ggccaaattt gcgctgaata tcatcggcgc ttaatttaat tcggttaacg
    39481 cctgggattt tctcgatgat agcatcgacc agcaccgcaa tcggccccat atacgccgac
    39541 gtaccgtgcg gaatagcaat accttccagc tcaaagaaat ttcgggtatc gcgggcaaac
    39601 cagtcgccga gcttataaac aaacgccgcg tgaattacca cacctgccat ccctatcatc
    39661 catgaaccgg ttgccagatg cagcaacgcg ccggtgaagg tcatatgcca gatattccag
    39721 atatcaacat ttaccacccg cgtcatacgg gtcagtaaca tcgccacgtt aaccaatatc
    39781 gcaatcggaa tcgccaccag cgcaatttgc gaagcccagg tcattggtga agagcccggc
    39841 cagccgacat cgaccacatg cagattcagg tcgaaatttt ccgccatcgc tttcgccgcc
    39901 ggaccaatgg aatccagcat taagccaatc acaaggccaa tgccaacaaa gccaatcccg
    39961 atatgcagac ccgctttaaa gcaatcgcct gccttcatgc ctaatatttt agaaaaaata
    40021 atgatgacaa ccggcagcat cactgtaggg ccgaggtcga gaatataacg catgacttct
    40081 gaaaacatag gtcacccctg taagatagtc agaat
//
LOCUS       DAJOSY010000028        38710 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJOSY010000028 DAJOSY010000000
VERSION     DAJOSY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.028"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(44..901)
                     /gene="murI"
                     /locus_tag="OHN27_001623"
     CDS             complement(44..901)
                     /gene="murI"
                     /locus_tag="OHN27_001623"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCQ2286080.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(846..2690)
                     /gene="btuB"
                     /locus_tag="OHN27_001624"
     CDS             complement(846..2690)
                     /gene="btuB"
                     /locus_tag="OHN27_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCQ2286081.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAYTHGYDVVAYGNTGTQAQPDKDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2755..2945)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3059..4159
                     /gene="trmA"
                     /locus_tag="OHN27_001625"
     CDS             3059..4159
                     /gene="trmA"
                     /locus_tag="OHN27_001625"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCQ2286082.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4199..4558)
                     /locus_tag="OHN27_001626"
     CDS             complement(4199..4558)
                     /locus_tag="OHN27_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCQ2286083.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4558..5208)
                     /gene="fabR"
                     /locus_tag="OHN27_001627"
     CDS             complement(4558..5208)
                     /gene="fabR"
                     /locus_tag="OHN27_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCQ2286084.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5539..6939
                     /gene="sthA"
                     /locus_tag="OHN27_001628"
     CDS             5539..6939
                     /gene="sthA"
                     /locus_tag="OHN27_001628"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCQ2286085.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFIIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(6922..7839)
                     /gene="oxyR"
                     /locus_tag="OHN27_001629"
     CDS             complement(6922..7839)
                     /gene="oxyR"
                     /locus_tag="OHN27_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCQ2286086.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8106..9479)
                     /gene="argH"
                     /locus_tag="OHN27_001630"
     CDS             complement(8106..9479)
                     /gene="argH"
                     /locus_tag="OHN27_001630"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCQ2286087.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9540..10316)
                     /gene="argB"
                     /locus_tag="OHN27_001631"
     CDS             complement(9540..10316)
                     /gene="argB"
                     /locus_tag="OHN27_001631"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCQ2286088.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10324..11328)
                     /gene="argC"
                     /locus_tag="OHN27_001632"
     CDS             complement(10324..11328)
                     /gene="argC"
                     /locus_tag="OHN27_001632"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCQ2286089.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11482..12633
                     /gene="argE"
                     /locus_tag="OHN27_001633"
     CDS             11482..12633
                     /gene="argE"
                     /locus_tag="OHN27_001633"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCQ2286090.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            13231..15882
                     /gene="ppc"
                     /locus_tag="OHN27_001634"
     CDS             13231..15882
                     /gene="ppc"
                     /locus_tag="OHN27_001634"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCQ2286091.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            16064..17797
                     /locus_tag="OHN27_001635"
     CDS             16064..17797
                     /locus_tag="OHN27_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCQ2286092.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            18012..18863
                     /locus_tag="OHN27_001636"
     CDS             18012..18863
                     /locus_tag="OHN27_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2286093.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18850..19191)
                     /locus_tag="OHN27_001637"
     CDS             complement(18850..19191)
                     /locus_tag="OHN27_001637"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCQ2286094.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19193..20071)
                     /locus_tag="OHN27_001638"
     CDS             complement(19193..20071)
                     /locus_tag="OHN27_001638"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCQ2286095.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLQLCGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(20037..22334)
                     /locus_tag="OHN27_001639"
     CDS             complement(20037..22334)
                     /locus_tag="OHN27_001639"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCQ2286096.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDAAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22385..22705)
                     /locus_tag="OHN27_001640"
     CDS             complement(22385..22705)
                     /locus_tag="OHN27_001640"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCQ2286097.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22720..23799)
                     /locus_tag="OHN27_001641"
     CDS             complement(22720..23799)
                     /locus_tag="OHN27_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCQ2286098.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAITAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            24108..26609
                     /gene="ptsP"
                     /locus_tag="OHN27_001642"
     CDS             24108..26609
                     /gene="ptsP"
                     /locus_tag="OHN27_001642"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCQ2286099.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26621..27283
                     /gene="fsa"
                     /locus_tag="OHN27_001643"
     CDS             26621..27283
                     /gene="fsa"
                     /locus_tag="OHN27_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCQ2286100.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKLLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27294..28397
                     /gene="gldA"
                     /locus_tag="OHN27_001644"
     CDS             27294..28397
                     /gene="gldA"
                     /locus_tag="OHN27_001644"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCQ2286101.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            28672..29289
                     /locus_tag="OHN27_001645"
                     /pseudo
     CDS             28672..29289
                     /locus_tag="OHN27_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(29316..30221)
                     /gene="yijE"
                     /locus_tag="OHN27_001646"
     CDS             complement(29316..30221)
                     /gene="yijE"
                     /locus_tag="OHN27_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCQ2286102.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFNYTLVIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(30315..32495)
                     /gene="katG"
                     /locus_tag="OHN27_001647"
     CDS             complement(30315..32495)
                     /gene="katG"
                     /locus_tag="OHN27_001647"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCQ2286103.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKAADESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDML"
     gene            complement(32824..33714)
                     /gene="metF"
                     /locus_tag="OHN27_001648"
     CDS             complement(32824..33714)
                     /gene="metF"
                     /locus_tag="OHN27_001648"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCQ2286104.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(34063..36495)
                     /gene="metL"
                     /locus_tag="OHN27_001649"
     CDS             complement(34063..36495)
                     /gene="metL"
                     /locus_tag="OHN27_001649"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCQ2286105.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNSTVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36498..37658)
                     /gene="metB"
                     /locus_tag="OHN27_001650"
     CDS             complement(36498..37658)
                     /gene="metB"
                     /locus_tag="OHN27_001650"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCQ2286106.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37935..38252
                     /gene="metJ"
                     /locus_tag="OHN27_001651"
     CDS             37935..38252
                     /gene="metJ"
                     /locus_tag="OHN27_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCQ2286107.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38436..>38710
                     /locus_tag="OHN27_001652"
     CDS             38436..>38710
                     /locus_tag="OHN27_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286108.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRV"
BASE COUNT         9458 a        10732 c         9741 g         8779 t
ORIGIN      
        1 ggaaatttaa aataattttc tgaccgcgca acattcaacc aaatcagcct aaaactgcca
       61 gtttttcgag cgtttcgaag ccgtaacgct gtaaaacggg caataattgt tcagcttctg
      121 gcgtcattgc catacaaaag gcaatattcg catcggcaga ttttgcatcc ggggcttcat
      181 gttctaacaa ccaggccgtt cggcgagcaa tcgctgcgcc agaatccacc agccgggttc
      241 cctctggcag cacttgtaac agttcttctt gtagtagagg gaaatgggtg caacccaata
      301 caacggtatc tggcggctct ttcattctta accacgggcg taggatacgt tttagtgcat
      361 ccagagaaac atcttcgcca tgtagcttcg cttcagccaa ctcaaccatc tctgccgagc
      421 ccagcatttc tatctggcat tcattagcga aacgcgcgat cagctcatga gtataagaac
      481 gtttaactgt tccgcgggtt gccagtaatc cgacaatgcc atttgccgtc agacgtgcag
      541 caggtttaat cgccggcacg acaccaacaa ccgggaagtc gaacttttcg cgtaatgcag
      601 gaagtgaaac ggtactggca gtgttgcaag cgaccacagc cagcgcaagg ggataacgtt
      661 cttgcaccgc ggtgacaatt gccaccactc gctcaacaat aaacgcttcg cttttttcgc
      721 catacgggaa agcgacgtta tcgaaagcat aaatgtaatg gagatccggt aagagatgcc
      781 ggatctcgtc atagaccgac aacccaccga cgccggagtc aaacaccagc acggtgggac
      841 gtggttcaga aggtgtagct gccagacaag gtgtattccc gtcctgcagt ttggtagcca
      901 tagactgtct cataatcttt gtcgaacagg ttggctattt taccacgaac tgtcaggtga
      961 gaggtgaccg gatacgcaac cgcaagatcc cacaagctca caccgcccat tttaacggtt
     1021 tgataaggat aagatgagta atccttatca tagcgagtgc ctaaatactg ataagtaata
     1081 ccccagtcga agtcatacaa ctgccagtcg agctggtatt tcacctgctg tttagcacgg
     1141 cgtaacaacg gcgtgtcggt aatcgcattg cgcgcatcga cataatcata actcacagta
     1201 tgcgtcagtg gtccggtatc aaaattggcg gtcgcctcga cgcccttaat ccgcgctttc
     1261 ccttcgttgt aatatttcag ggtgtgatca tcataatcga tcaagtcact gacatcgtta
     1321 cgatatccgg aaatacgcca gttcacccca gcggttaagc cttcaaacgc gccttcccac
     1381 tgtttgcttt tctccgggtc cagattcgga tttccgtaga agccatacag ttgccccaga
     1441 tttggtgcct tataagatgt cccgtaggaa gcaatgaagc gataaccttc gatgaattcc
     1501 caaccggcgc tggtttgcca ggttccatga cgaccaaact gtgagttatc gtcgctgcgt
     1561 gctgcgcctt caaaggtaaa atcgccgact tgttgcagcc cggtcagata gatgccagta
     1621 ttacgttgat catatccatc ctcaacataa cctgtacccg gcgtcgtagt ctgtttctgc
     1681 cagtcgacac ccgcaccaat actaccgtga ccaacgatga cattgtttgc ccactggacg
     1741 gtgtattgct tcatctcatc gagcgtcgcc gacgaatcat aacgaccata atggggatcg
     1801 tagttgtaat ctttgctatg gctatagctg gtaatgagtt gtgatttaat cagttcgccg
     1861 ttatagcgca gcccggcgtc ccaactttga ctatagagtt tacgggtatc gaccaatggt
     1921 gaacccggag aataatacgc gtcataattg gtacggttat catagccata gccgcgcaca
     1981 aagccgctcc aggcatcagt aaagttatgc tccagcgcgc cataaagcgt tttacttaaa
     2041 aaaccatctt tatctggctg cgcttgcgtt ccggtattac cataggcaac aacatcataa
     2101 ccatgagtat aggcataatc gcccaacaac gttactcgtg tcttatcccc cagttgttgc
     2161 tgtgtagaga catcatagtt ttgataacta ttgcttcccc accctgctga aatttccgtt
     2221 ccgggttcat cgcgcgtcgt gatgatattc accaccccac ctattgcatc ggaaccataa
     2281 acggcggagc gtggcccacg gatatattca acacgctgga caagcgcaat agggaactgg
     2341 ctaaggtcgg cagaaccact cacccccgcc agattcaggc gtacgccatc aattaacacc
     2401 aacacatgac tggcatttgt accgcgaata aaaatagatg agagctgacc tgaaccgccg
     2461 ttttgggtga tatcgacgcc cggaagacgg cgcagcacat cattaaccga ggtcgactgc
     2521 cagcggtcga tatcctgacg cgtcacaacg gtggttggtg caagcacagt gctgcgcggc
     2581 tgttcaaaac ggttagcagt aacgacgaga gtatccgggc tggtatcctg tgcccaagcg
     2641 gaaaaggctg tgacagaaca cgccgtcagc agcgaagctt ttttaatcat tgtaaagcat
     2701 ccacaataga agaaggatgc cgcaggtttc atcaatatta cgcgatgatg agaaccagat
     2761 gcgacgttgg ccggcaggtc ttcgggcttg gaggggtatc taagatacta agagatgatg
     2821 acttcccacc gaatggcagt gtccgcataa cgcaatcatc gcacctttcc ttaccgctgc
     2881 gcgtcagctc cagattcgca ctggattccc tattaactca caggaccggc aagtggatgc
     2941 tacaggttgt aacaagttac tgtccagacg tagctcacaa ataggaattc atcaagatct
     3001 ggacatctga tgagcaatcc ctacaatcgc cgcgtacttt aatttttcag gatacatcat
     3061 gacccccgaa caccttccaa cagaacagta tgaagcgcag ttagccgaaa aagtggtacg
     3121 tttgcaaagt atgatggcac cgttttctga cctggttccg gaagtgtttc gctcgccggt
     3181 cagtcattac cggatgcgcg cggagttccg catctggcac gatggcgatg acctgtatca
     3241 catcattttc gatcaacaaa ccaaaagccg catccgcgtg gatagcttcc ccgccgccag
     3301 tgaacttatc aaccagttga tgacggcgat gattgcgggt gtgcgtaata atcccgttct
     3361 gcgccacaag ttgttccaga ttgattacct cactacgctg agtaatcagg cggtggtttc
     3421 cctgctatac cataagaagc tggatgatga gtggcgtcag gaagcggagg ccctgcgcga
     3481 tgcactgcgc gcgcagaatc tgaatgtgca tctgattggt cgggcaacga aaaccaaaat
     3541 cgagctggat caggattaca tcgatgaacg tctgccggtc gcagggaaag agatgatcta
     3601 ccgtcaggta gaaaacagct ttacccagcc gaacgcggcg atgaatattc agatgctgga
     3661 atgggcgctg gacgtaacca aaggctcaaa aggcgattta ctggagctgt actgcggcaa
     3721 cggtaacttt tcattagcgc tggcgcgcaa ttttgatcgg gtattagcca ccgaaatcgc
     3781 taagccgtcg gttgctgctg cgcaatacaa catcgcagct aaccatattg ataacgtaca
     3841 aattattcgt atggcggcag aagaatttac tcaggcgatg aatggtgtgc gcgagtttaa
     3901 ccgcctgcaa gggatcgact taaagagtta tcagtgcgaa accatttttg tcgacccacc
     3961 gcgcagcggt ctggacagtg aaaccgagaa aatggtgcag gcgtatccgc gtattttgta
     4021 catctcctgt aacccggaaa cgttatgtaa gaatctggaa acattaagcc agacgcacaa
     4081 ggtcgaacgt ctggctctgt ttgatcagtt cccctacacg caccatatgg agtgcggcgt
     4141 attactgacc gcgaagtaaa acccatgccg gatgcgccag catccggcat aataccgatt
     4201 actcagcaac ttcctgctta cggttacgca tcttcgcgcc aatccagaac accataatga
     4261 cggagagtac tgccgggaag aagttagagc cgatatccgg atattccgca cgaactaccg
     4321 tgctgtacag cagtacgccg agaataaaac aggcagctgc cagacccggc aagcctaccg
     4381 gcatagtgcg attaagataa cgttgatgca ggcagtaaac cgtcagcacc agggaaataa
     4441 tcgggaatac agaaaatggc acaatggagc taaacagcgc tgcgaaagta ccattaatcg
     4501 ataagccagc aactaacgcc agcagcagcg tacctctatc ttgatttgct tgtttcatta
     4561 ctcgtccttc acatttcccg gaataattgc ggttttctct tgttcacggc gataccagta
     4621 ataagcccct ttcgaaatca ttcgcagttg cagtaccagt cgctcttcta attgccgacg
     4681 ttgttcgacg ccgacgtcca acgcctcggc acccgcactg aagacaattg tcaccattgc
     4741 ttcggcttgc gcttcagtaa acgcacgcgg catatggttt tcgagttcca gatagtccgc
     4801 aagttccgca atgaagtgct gaatttcacg cgcaacggcg gcacgaaacg cagcggaggt
     4861 gccggagcgt tcccgcaata ataaccggaa ggcgttagga ttattaccga tgaactccat
     4921 aaatgtggag accgaggtgc ggatcacact cccgcctttg gcgatacgct gacgcgcctg
     4981 gcgcatgagt tggcgtagca ttaaaccgct ctcatcaacc atggtcagac ccagttcgtc
     5041 tacgtcgcgg aaatgccgat aaaaagaggt gggagcaatg cccgcttcac gcgccacttc
     5101 acgcaaactc aggctggcga agctgcgttc agcacttaat tggctaaatg cggcttccac
     5161 cagcgaacgg cgggtttttt ctttttgttg cgctcttacg cccatcacga tgtctgaatc
     5221 cttgccaaaa gtactgctgg cactatacca gagaatgaac atactggatt gctccagtat
     5281 tgtgaatgaa cggtaacgca ataaaacata tttactgcaa ttctttggcc ggttctttta
     5341 cgtacagcgg aaacctgccg cttaaacgga gagtatcgtc gataaaaatc caataaaacg
     5401 tcagggcaaa agtaagaaac agacaaagca aaggccgctc aggatatagc cagataaatg
     5461 acggggatca attggcttac ccgcgataaa atgttaccat tctgttgctt ttatgtataa
     5521 gaacaggtaa gccctaccat gccacattcc tacgattacg atgccatagt aataggttcc
     5581 ggccccggcg gcgaaggcgc tgcaatgggc ctggttaagc aaggtgcgcg cgtcgcagtt
     5641 atcgagcgtt atcaaaatgt tggcggcggt tgcacccact ggggcaccat cccgtcgaaa
     5701 gctctccgtc acgccgtcag ccgcattata gaattcaatc aaaacccact atacagcgac
     5761 cattcccggc tgctccgctc ttcttttgcc gatatcctta accatgccga taacgtgatt
     5821 aatcaacaaa cgcgcatgcg tcagggattt tacgaacgta atcactgtga aatattgcag
     5881 gggaacgctc gctttgttga cgagcatacg ttggcgctgg attgcccgga cggcagcgtt
     5941 gaaacactaa ccgctgaaaa atttattatt gcctgcggct ctcgtccata tcatccaaca
     6001 gatgttgatt tcacccatcc acgcatttac gacagcgact caattcttag catgcaccac
     6061 gaaccgcgcc atgtacttat ctatggtgct ggagtgatcg gctgtgaata tgcgtcgatc
     6121 ttccgcggta tggatgtaaa agtggatctg atcaacaccc gcgatcggct gctggcattt
     6181 ctcgatcaag agatgtcaga ttctctctcc tatcacttct ggaacagtgg cgtagtgatt
     6241 cgccacaacg aagagtacga gaagatcgaa ggctgtgacg acggtgtgat catgcatctg
     6301 aagtcgggta aaaaactgaa agctgactgc ctgctctatg ccaacggtcg caccggtaat
     6361 actgattcgc tggcgttaca gaacattggg ctggaaactg acagtcgcgg acagctgaag
     6421 gtcaacagca tgtatcagac cgcacagccg cacgtttacg cggtgggcga cgtgattggt
     6481 tatccgagcc tggcgtcggc ggcctatgac caggggcgca ttgccgcgca ggcgctggtg
     6541 aaaggtgaag ccaccgcaca tctgattgaa gatatcccta ccggcattta caccatcccg
     6601 gaaatcagct ctgtgggcaa aaccgaacag cagctgaccg cgatgaaagt gccatatgaa
     6661 gtgggccgcg cccagttcaa acatctggca cgtgcacaaa tcgtcggcat gaacgtgggc
     6721 acgctgaaaa ttttgttcca tcgggaaaca aaagagattc tgggcattca ctgctttggc
     6781 gagcgcgctg ccgaaattat tcatatcggt caggcgatta tggaacagaa aggtggcggc
     6841 aacactattg agtacttcgt caacaccacc tttaactacc cgacgatggc ggaagcctat
     6901 cgggtagctg cgctaaatgg cttaaaccgc ctgttttaac actttatcga aatggccatc
     6961 cattcttgcg cggatggcct ctgccagctg ctcatagcgg ctgcgcagcg gtgagccagg
     7021 acgataaacc aggccaatcg tgcggcgagg ttccggctta atacacggca aataaacaac
     7081 cccatcgcgt ttgcgctccg gcggcacagc cagcgcaggc agtaaagtga tcccgctacc
     7141 tgccgccacc atgttacgca gtgtctccag gctggtcgcg cggaagtgtg tatcttcatc
     7201 cgccccggct tcaaagcaga aacccattgc ctgatcgcgc aaacagtgac catcttccag
     7261 catcagcagt ttttcccctg ccagatcggc catcggtacg cattcgcggt tcgcccacgg
     7321 gtgatcttca tagatagcca gcaacattgg ctcatcaaac aacggcactt caatgaatgc
     7381 ttcgctctct ttcaccagcg cgaggatcac gcaatcgagt ttgccactgt ccagttgcgc
     7441 cagtaactgg tgggtctgtg cttcatgcag atacatttcc agctttggaa aggtctggtg
     7501 cagcatcggg ataatatgcg gtagcaggta cggtccaacc gtgggaatca aaccaatatg
     7561 cagcggtccg gacatcgtct cgccctgctg gcttgccatc tctttaagga ctttcacctc
     7621 acgcagcacg gtacgcgcct gatccaccag cagcattccc gcctgggtga acaacacttt
     7681 acggctggtc cgctccagca acatcacgcc caactcatct tccagcttac gaatttgccc
     7741 gctaagcgtc ggctggctaa cgtggcagga atctgccgca cgccgaaaat ggcggtgttc
     7801 agccaatgcc accaggtact caagatcacg aatattcatt atccatcctc catcgccacg
     7861 atagttcatg gcgataggta gaatagcaat gaacgattat ccctatcaag cattctgact
     7921 gataattgct cacagaaacg gagcggcacc tcttttaacc cttgaagtca ctgcccgttt
     7981 cgagagtttc tcaactcgaa taactaaagc caacgtgaac ttttgcggat ctccaggatc
     8041 cgcttttttt tgccataaaa aagcccggcg ataagccagg ctcaaatttg tacatataaa
     8101 tgttcttacc ccaaccgagc ctgcgcaaaa gcaatcgcct gcgccacctg ctgcggtgag
     8161 acgccgcctt ttgccgcacg cttgtcgagg cacgattgca gcgacagaat cggatagaca
     8221 tcttcgtcaa tcacctgact gaatttctgc aactcactga gcggcagatc ttccagcggt
     8281 ttgccctgac gaatggcttc caccaccgct tcaccaacaa tatggtgcgc ctcgcggaac
     8341 ggtacgcctt tcgccaccag ataatccgcc agttcggtgg cgttggcgta accctgctga
     8401 gccgcttcct ggcaacgtgg acgtttcacc tgaatgccgt ccagcaccag cgccgccata
     8461 tgcaggcagt ccagccaggt atcgagcgcg tcgaacagac cttctttgtc ttcctgcata
     8521 tctttgttgt aagccagcgg caaacctttc agcgtcatca tcatgccggt taacgccccc
     8581 tgcacccgac cgcatttacc gcgaatcagc tccagcgcat ccgggttttt cttctgcggc
     8641 attaatgatg aaccggaagt cacgcggtca gaaagctcca caaaccccgc ttcgccggtg
     8701 ttaaagaaaa tcagatcttc agcaaaacgc gacagatgca ccatgccgat agcggcagca
     8761 gaaagcagtt ccaacacatg gtcacggtca gaaacgctgt cgagactgtt acgggtcgcc
     8821 gaagcaaagc ccagccagcc tgctaactgt tcacggtcga tttcataggc cgttcccgcc
     8881 agcgcgccac agcctagcgg gctgacatcc agacgcttaa gcgcatcctg caaacggctt
     8941 tcatcacgcg ccagcatctc aacataggcc aggcaccagt gcgcgaacgt caccggctgg
     9001 gcgcgttgca ggtgagtgta acctggcatt accgcgtcct gattgttttg tgcggtttcc
     9061 accagcgccg attgcagctg ccggttagcc gtcagtaact cgctaacggt atctttgcac
     9121 cacagtttca ggtcagtcgc tacctgatca ttacggctac gcccggtatg cagctttttg
     9181 cctaactggc ccactttgtc gatcagtttg ccttccaccc agctatggat atcttcggcg
     9241 tcgctttcaa ggatttgttg tggcctggcg cgaacatctt ccagcaacac gttcagcgcc
     9301 tcttccagtt gcgcctgctc ttctgcggtt aacacgccta ccgtgaccag ggctttggac
     9361 caggccacag agccaacaat atcctgctcc gccagacggt aatcaaagcg cagtgagtcg
     9421 ttgaattgtt tgaaccgttg atctgctgcc tgggtaaaac gcccgcccca aagtgccata
     9481 actctgtttc cttattttga aattcaatgc cggaaagctg cgcctccggc caacaaaact
     9541 taagctaaaa tccgcgtacc catcggcata ccgttaaaca gtgccggaag ctgctccgca
     9601 tgacgccagg aggcgatatc taccggacgg cccagcgtgc gggccgcatc cagcgccgcg
     9661 ttcactttca ctatcatgcc gtcagtaata atgccctgct caatcagttg ttctgctttc
     9721 gcggcggtca tttcggcaat gcgttgccct ttgccgtcga gaatgccgct gacgtcggag
     9781 agcaaaatca gatccgcgcc cagcgttgcc gccagcgccg ttgccgcctg gtcggcattg
     9841 acgttcatca gttgcccttc gtctgttacg ccaatggagc tgaccaccgg cagataaccg
     9901 ttctccagca aggagttgat aagcttaggc gaacctggct gcgccagtcc aacatgacct
     9961 aactcttcat caagctgggt cactttgacg ctgtcgccgt caccgagaaa caaacctacg
    10021 gccgcaatct gatgtttctt cgcccatgcc aacagggttt tatttgccgt tcccgccagt
    10081 gctccggtga taatgtctat ctgatcagca ggcgtcaccc gcaggccgtt tttctttttc
    10141 accggcagat tcagcccttt catcagctca tccaccacgc aaccgccgcc gtgcacaatc
    10201 accagcggac gctgatgtga ctcacgataa ttcaccagtg cgctaaacag acgttccagc
    10261 gcctcttcac tatccagcag tacgccgccc agtttgataa ttaatggatt catcattgca
    10321 cccttaaata agagactgcg tttccgcata gccgaaacga atattggcgc actgtaccgc
    10381 ttgtgccgcc gcgcctttca gtaagttgtc ttcggtcgcc acaataatca aatgctcgcc
    10441 ctgaacggca aacccgatat cgcaaaatgg cagcccaacg acatttttca gcgccggaac
    10501 gcctttgtca tacagccgca ccagcggttt atgggcatac gcctgctgta acacttgcgc
    10561 gacttgcgcc tgggtcacac ccgatttcag gcggcaggta atggtttcga gaatgccgcg
    10621 cgggaaattg cccagatgtg gggtgaagat aacgtcagca ccgaggtgtg tggcgatctc
    10681 tggttgatgg cgatgagtaa agacgccata cggttgcagg ctaacttcac aaaagctgtt
    10741 tgaaatggcc gctttacgcc ctgcaccgct cacgccgctg gtggcgttga tcaccggcca
    10801 ctgattgagg tcaagaagat cggcatcaat caacggtttc agcgccagct gtgccgccgt
    10861 cggataacag cccggcaccg caatcaaatt cgcttctttt aatttattac cgcaccactc
    10921 cgccagaccg taggctgcct gttccaacag ttccgggtat tgatgggtaa agccgtaata
    10981 tttttcatag aaggtggcgt cgttaacacg aaacgcgccg gaaaggtcga acaccacgca
    11041 gcccgcttca agaaactgcg gcgctaaatc gtggctaact tcatgggcgg tggcgagaaa
    11101 cactacgtcc acccctgggc taaactcgct gatatccgac atcggctgca acggcagatc
    11161 aacgatgcct tttagctgcg gatgcaaatc ggagattaac tttcccgcat cattgctttg
    11221 cgctgaaaca gtcaaagcgg ttatgttcat atgcggatgg cgatttacat aggtcactag
    11281 ctctgcgcca gcgtagccgc tggcacccac aatcagcgta ttcaacatcg gggctattca
    11341 ccttcttatg tctggttgcc aggttaaacg taaaacattc accttacggc tggtgggttt
    11401 tattacgctc aacgttagtg tatttttatt cataaatact gcatgaatat tgatactatc
    11461 atgaccagag gtgtgtcaac aatgaaaaac aaattaccgc catttatcga gatttaccgc
    11521 gctctgattg ccacaccttc aataagcgcc acggaagagg cactcgatca aagcaatgca
    11581 gatttaatca ctctgctggc ggactggttt aaagatttgg gcttcaatgt ggaagtgcag
    11641 cctgttccag gaactcgcaa caaattcaat atgctggcaa gtatcggaca gggggctggc
    11701 ggcttgttgc tggcggggca taccgatacg gtgccatttg atgacggtcg ctggacgcgc
    11761 gatccgttta cactgacgga gcatgacggc aagctttacg gcttaggcac cgccgacatg
    11821 aaaggctttt ttgcgtttat ccttgatgcg ctacgcgatg tcgacgtcac gaaactgaaa
    11881 aaaccgctct acattctggc gactgctgat gaagaaacca gtatggccgg agcgcgttat
    11941 tttgccgaaa ctaccgccct gcgcccggat tgcgccatca ttggcgaacc gacgtcacta
    12001 caaccggtac gcgcacataa aggtcatatc tctaacgcca tccgtattca gggccagtcg
    12061 gggcactcca gcgatccagc acgcggagtt aacgctatcg aactaatgca cgacgccatc
    12121 gggcatattt tgcaattgcg cgataacctg aaagaacgtt atcactacga agcgtttacc
    12181 gtgccatacc ctacgctcaa cctcgggcat attcacggtg gcgacgcttc taaccgtatt
    12241 tgcgcttgct gtgagttgca tatggatatt cgtccgctgc ctggcatgac actcaatgaa
    12301 cttaatggtt tgctcaacga tgcattggct ccggtgagcg aacgctggcc gggtcgtctg
    12361 acggtcgacg agctgcatcc gccgatccct ggctatgaat gcccaccgaa tcatcaactg
    12421 gttgaagtgg ttgagaaatt gctcggagca aaaaccgaag tggtgaacta ctgtaccgaa
    12481 gcgccgttta ttcaaacgtt atgcccgacg ctggtgttgg ggcctggctc aattaatcag
    12541 gctcatcaac ctgatgaata tctggaaaca cggtttatca agcccacccg cgaactgata
    12601 acccaggtaa ttcaccattt ttgctggcat taaaacgtag gccggataag gcgctcgcgc
    12661 cgcatccggc gctgttgcca aactccagtg ccgcaataat gtcggatgcg atgcttgcgc
    12721 atcttatccg acctacagtg actcaaacga tgcccaaccg taggccggat aaggcgctcg
    12781 cgccgcatcc ggcactgttg ccaaactcca gtgccgcaat aatgtcggat gcgatacttg
    12841 cgcatcttat ccgaccgaca gtgactcaaa cgatgcccaa ctgtaggccg gataaggcgc
    12901 tcgcgccgca tccggcactg ttgccaaact ccagtgccgc aataatgtcg gatgcgatac
    12961 ttgcgcatct tatccgacct acacctttgg tgttacttgg ggcgattttt taacatttcc
    13021 ataagttacg cttatttaaa gcgtcgtgaa tttaatgacg taaattcctg ctatttattc
    13081 gtttgctgaa gcgatttcgc agcatttgac gtcaccgctt ttacgtggct ttataaaaga
    13141 cgacgaaaag caaagcccga gcatattcgc gccaatgcga cgtgaaggat acagggctat
    13201 caaacgataa gatggggtgt ctggggtaat atgaacgaac aatattccgc attgcgtagt
    13261 aatgtcagta tgctcggcaa agtgctggga gaaaccatca aggatgcgtt gggagaacac
    13321 attcttgaac gcgtagaaac tatccgtaag ttgtcgaaat cttcacgcgc tggcaatgat
    13381 gctaaccgcc aggagttgct caccacctta caaaatttgt cgaacgacga gctgctgccc
    13441 gttgcgcgtg cgtttagtca gttcctgaac ctggccaaca ccgccgagca ataccacagc
    13501 atttcgccga aaggcgaagc tgccagcaac ccggaagtga tcgcccgcac cctgcgtaaa
    13561 ctgaaaaacc agccggaact gagcgaagac accatcaaaa aagcagtgga atcgctgtcg
    13621 ctggaactgg tcctcacggc tcacccaacc gaaattaccc gtcgtacact gatccacaaa
    13681 atggtggaag tgaacgcctg tttaaaacag ctcgataaca aagatatcgc tgactacgaa
    13741 cacaaccagc tgatgcgtcg cctgcgccag ttgatcgccc agtcatggca taccgatgaa
    13801 atccgtaagc tgcgtccaag cccggtagat gaagccaaat ggggctttgc cgtagtggaa
    13861 aacagcctgt ggcaaggcgt accaaattac ctgcgcgaac tgaacgaaca actggaagag
    13921 aacctcggct acaaactgcc cgtcgaattt gttccggtcc gttttacttc gtggatgggc
    13981 ggcgaccgcg acggcaaccc gaacgtcact gccgatatca cccgccacgt cctgctactc
    14041 agccgctgga aagccaccga tttgttcctg aaagatattc aggtgctggt ttctgaactg
    14101 tcgatggttg aagcgacccc tgaactgctg gcgctggttg gcgaagaagg tgccgcagaa
    14161 ccgtatcgct atctgatgaa aaacctgcgt tctcgcctga tggcgacaca ggcatggctg
    14221 gaagcgcgcc tgaaaggcga agaactgcca aaaccagaag gcctgctgac acaaaacgaa
    14281 gaactgtggg aaccgctcta cgcttgctac cagtcacttc aggcgtgtgg catgggtatt
    14341 atcgccaacg gcgatctgct cgacaccctg cgccgcgtga aatgtttcgg cgtaccgctg
    14401 gtccgtattg atatccgtca ggagagcacg cgtcataccg aagcgctggg cgagctgacc
    14461 cgctacctcg gtatcggcga ctacgaaagc tggtcagagg ccgacaaaca ggcgttcctg
    14521 atccgcgaac tgaactccaa acgtccgctt ctgccgcgca actggcaacc aagcgccgaa
    14581 acgcgcgaag tgctcgatac ctgccaggtg attgccgaag caccgcaagg ctccattgcc
    14641 gcctacgtga tctcgatggc gaaaacgccg tccgacgtac tggctgtcca cctgctgctg
    14701 aaagaagcgg gtatcgggtt tgcgatgccg gttgctccgc tgtttgaaac cctcgatgat
    14761 ctgaacaacg ccaacgatgt catgacccag ctgctcaata ttgactggta tcgtggcctg
    14821 attcagggca aacagatggt gatgattggc tattccgact cagcaaaaga tgcgggagtg
    14881 atggcagctt cctgggcgca atatcaggca caggatgcat taatcaaaac ctgcgaaaaa
    14941 gcgggtattg agctgacgtt gttccacggt cgcggcggtt ccattggtcg cggcggcgca
    15001 cctgctcatg cggcgctgct gtcacaaccg ccaggaagcc tgaaaggtgg cctgcgcgtg
    15061 actgaacagg gcgagatgat ccgctttaaa tatggtctgc cagaaatcac cgtcagcagc
    15121 ctgtcgctgt acaccggggc gattctggaa gccaacctgc tgccaccacc tgagccgaaa
    15181 gagagctggc gtcgcattat ggatgaactg tcagtcatct cctgcgatct ctaccgtggc
    15241 tacgtacgtg aaaacaaaga ttttgtgcct tacttccgct ccgctacgcc ggaacaagaa
    15301 ttgggcaaac tgccgttggg ttcacgtccg gcgaaacgtc gcccaaccgg cggcgtcgag
    15361 tcactacgcg ccattccgtg gatcttcgcc tggacgcaaa accgtctgat gctccccgcc
    15421 tggctgggtg caggtacggc gctgcaaaaa gtggtcgaag acggcaaaca gagcgagctg
    15481 gaggctatgt gccgcgattg gccattcttc tcgacgcgtc tcggcatgct ggagatggtc
    15541 ttcgccaaag cagacctgtg gctggcggaa tactatgacc aacgcctggt agacaaagca
    15601 ctgtggccgt taggtaaaga gttacgcaac ctgcaagaag aagacatcaa agtggtgctg
    15661 gcgattgcca acgattccca tctgatggcc gatctgccgt ggattgcaga gtctattcag
    15721 ctacggaata tttacaccga cccgctgaac gtattgcagg ccgagttgct gcaccgctcc
    15781 cgccaggcag aaaaagaagg ccaggaaccg gatcctcgcg tcgaacaagc gttaatggtc
    15841 actattgccg ggattgcggc aggtatgcgt aataccggct aatcttcctc ttctgcaaac
    15901 cctcgtgctt ttgcgcgagg gttttctgaa atacttctgt tctaacaccc tcgttttcaa
    15961 tatatttctg tctgcatttt attcaaattc tgaatatacc ttcagatatc cttaaggaat
    16021 tgtcgttaca ttcggcgata ttttttcaag acaggttctt actatgcatt ccacagaagt
    16081 ccaggctaaa cctcttttta gctggaaagc cctgggttgg gcactgctct acttttggtt
    16141 tttctctact ctgctacagg ccattattta catcagtggt tatagtggca ctaacggcat
    16201 tcgcgactcg ctgttattca gttcgctgtg gttgatcccg gtattcctct ttccgaagcg
    16261 gattaaaatt attgccgcag taatcggcgt ggtgctatgg gcggcctctc tggcggcgct
    16321 gtgctactac gtcatctacg gtcaggagtt ctcgcagagc gttctgtttg tgatgttcga
    16381 aaccaacacc aacgaagcca gcgagtattt aagccagtat ttcagcctga aaattgtgct
    16441 tatcgcgctg gcctatacgg cggtggcagt tctgctgtgg acacgcctgc gcccggtcta
    16501 tattccaaag ccgtggcgtt atgttgtctc ttttgccctg ctttatggct tgattctgca
    16561 tccgatcgcc atgaatacgt ttatcaaaaa caagccgttt gagaaaacgt tggataacct
    16621 ggcctcgcgt atggagcctg ccgcaccgtg gcaattcctg accggctatt atcagtatcg
    16681 tcagcaacta aactcgctaa caaagttact gaatgaaaat aatgccttgc cgccactggc
    16741 taatttcaaa gatgaatcgg gtaacgaacc gcgcacttta gtgctggtga ttggcgagtc
    16801 gacccagcgc ggacgcatga gtctgtacgg ttatccgcgt gaaaccacgc cggagctgga
    16861 tgcgctgcat aaaaccgatc cgaatctgac cgtgtttaat aacgtagtta cgtctcgtcc
    16921 gtacaccatt gaaatcctgc aacaggcgct gacctttgcc aatgaaaaga acccggatct
    16981 gtatctgacg cagccgtcgc tgatgaacat gatgaaacag gcgggttata aaaccttctg
    17041 gatcaccaac cagcagacga tgaccgcccg caataccatg ctgacggtat tttcgcgcca
    17101 gaccgacaag cagtactaca tgaaccagca acgtacgcag agtgcgcgtg aatacgacac
    17161 caacgtgctg aagccgttcc aggatgtgct gaatgaccct gcgccgaaga aactgatcat
    17221 cgttcatctg ctgggtacgc atatcaaata caaataccgc tacccggaaa atcagggcaa
    17281 gtttgatggc aataccgatc atgttccgcc aggattaagc gcagaagagc tggaatcata
    17341 taacgattat gacaacgcta acctgtataa cgatcatgtg gttgccagcc tgattaaaga
    17401 ctttaaagcg gcagacccga acggattcct ggtttacttc tctgaccacg gtgaagaggt
    17461 ttacgacacg ccgccgcaca aaactcaggg gcgtaacgaa gacaacccga cgcgccacat
    17521 gtacaccatt ccgttcctgc tgtggacgtc ggaaaaatgg caagcgactc atccccgtga
    17581 tttctcacag gatgtcgatc gtaaatacag cctggcggaa ctgatccaca cctggtcaga
    17641 tttggcgggc ttatcttacg acggttacga tccaacccgt tcagtggtga atccgcagtt
    17701 caaagaaact acccgctgga ttggtaaccc gtacaagaaa aacgcgctga tcgattacga
    17761 cactctgccg tatggcgacc aggtaggtaa tcagtaatta tcagccaggc gctatttgcc
    17821 ggatgcgacg cttgacgcgt cttatccggc ctacagatcg cggcggtagg ctggataagg
    17881 cgtttacgcc acatccggca atacaactgc cgcaatgttt acatcgaact cccctcaggc
    17941 cacacttttc aggtggcctt ttttaataat gccagttact gttttcccgt ctacatacct
    18001 aagggaataa catgtatcac gacgtcagct atctgctctc ccgcctgatc aacggcccgc
    18061 tgtccctgcg ccagatttac tttgccagca gtaacggccc ggtgcccgat cttgcgtatc
    18121 aggtcgattt tccacggctg gaaattgtgc tggaaggtga gtttgttgat accggcgctg
    18181 gagcaacgtt agttcccggc gatgtgctgt acgttgccgc tggtggctgg aatttcccac
    18241 aatggaaaac acccgctacc acctttagcg tgctgtttgg caaacagcaa ctcggcttca
    18301 gcgtcgtgca atgggatggc aaacaatatc aaaatctggc gaagcaacac gtcgcccgac
    18361 gcggcccacg tataggttct tttctgctac aaacgctcaa tgaaatgcaa atgcagccgc
    18421 aggagcagca aacggcaagg cttatcgtag ccagcctgct tagccactgc cgcgatttgc
    18481 tcggcagcca aatccagacc gcctcacgca gccaggcact atttgaagct attcgcgatt
    18541 atatcgacga acgctatgcc tccgcgctta cccgcgaatc tgtcgcacag gcattctata
    18601 tttcaccaaa ctatctctcg cacctgtttc aaaaaacggg ggccattggt tttaacgaat
    18661 acctgaatca cacgcgactg gagcacgcta agacgttatt gaaaggctat gacctgaagg
    18721 taaaagaagt ggcgcacgcc tgcggctttg tcgacagcaa ctacttctgc cgtctgtttc
    18781 gtaaaaacac cgaacgctcg ccgtcggaat atcgccggca gtaccacagc caactgacag
    18841 aaaaaccgac tactccagaa taagatgggt ttgctgcggc gcagaaagca ctttgcgcac
    18901 cgcgctcatt acccgctgcg gctcacgcag gaatgcgtag atgctgcatt gcacatagcg
    18961 gcaatgttca aatcgctcgg caccggcaag ctcgatatcc gtaatcaaca gagcaacatc
    19021 cgcccgacgg atatcctcgt ccgctaaacg attctccgtt cccagcgcgc cctgagtttc
    19081 aatgctgact ccccacttct ctaacaggca caacttttcc agccgttccg ccgccatata
    19141 agtatgcgcc acgccactga cgcaggcggt tactgccacc aggtatgcca ttttaacctc
    19201 ccacggtaac ctgaaatccg gcccgttctg ccatttcgcg catcgttgcc acatcggctg
    19261 acgacggcgc aggcacctct ttcatcgacc atgttttccc cagcaggcgg tatttcggtt
    19321 cgccgtactg atgaaacggt aacagatgga tctgcctgat attcagcggg atcagcacat
    19381 ccagcgcctg ctgcatattc tcccggctga gcgtgaaacc agggatcagc ggtaaacgcg
    19441 ggatcacgtt gacgccctca ctcaccagca aacgcagatt ctccagcacg cgtggcaggt
    19501 tcatcttcac cacatcccgc gcctgagtcg cgtccatgat ttttaaatcg aacaacactt
    19561 catcgcacaa tttcgccagc ggtaacagct tgctggctgg cgcgtctccg gcagtttcaa
    19621 tggcacatga gacaccacac agctgcagtc gctgtaaaaa acgggtagca aactccgcct
    19681 gcattaacac ttcgccgcca gaaagcgtga cgccgccgcc ggacgtgcga aaaaaaatgt
    19741 catctttcat cacttcccgt tccagagcgt caaggctgat atcgcgacca atccgttcaa
    19801 acgccccgga ggggcattca tccgcatcac gcaaacattt cgcacagtgc agacatttcg
    19861 cctctctgcg taccgtctgg attttgccgg agatcgactc cggattagca caccacgggc
    19921 aaagatgcgg acagccttta aaaaagacca ccgtacgaat gccctcaccg tcattcagtg
    19981 aataacgctg aatgttgaaa atgcgcgcca catcattgcg gcgcgtttcc acaacgttac
    20041 agctgatgcg ctgtccggcg gatgatgtca tcctggatct ccttcgacag ttcgacaaag
    20101 aaggcgctgt atccggcaac gcgcaccacc agcccggcat aatcttgtgg gcgctgttgc
    20161 gcttcccgca acgtgtcggc gttcaccacg ttaaactgaa tatgttgtaa cttaagctgg
    20221 gtaaacgccc gtaagaagtc ggccagtttg cgtaatcccg cttcaccttc cagggtcgcc
    20281 ggagtgaatt tcacgttcag caatgtaccg ttagacagca gtgtgttatc gagcttactg
    20341 actgacttca gtaccgccgt tggcccttgt gcgtcctgac ccagcatagg tgacaagccg
    20401 ccgtctgcca gctgttctcc ggcaaaacga ccgtctggcg tcgcgccaac caccgatccc
    20461 aacggaacgt gagcagaaac ggtatacgat cccggcgtga agtagccgcc gcgcgggttc
    20521 tggtattttt ccacttcttt gcagtagtgg cgcaacagtt cggcgctaat gttatccacc
    20581 tcgtcgatat cgttaccgta tttctcaaag cggttaatta agcgagcgcg gactttttcg
    20641 ccttctggcg ttgcaaagtt ggcttttaat accgacagca attcgtcaaa acttaaacgc
    20701 tgttgatcaa aaaccatccc tttcaacgca tggagagaat cgctcaggtt ggcgataccg
    20761 atcccctgta cgccggagaa gttataacgc gcgccgccat cggtaatatc gcggcctttt
    20821 tccagacaat cgctgataaa agacgagagc agcggtacag gtgcccagtc gcgatggccg
    20881 atatcgcaaa tattactgcc ttcaaccatc agggtgatgt agtggctgat cttggcacgg
    20941 atctgttcca gtaaaccttc ataagtcagc gcggcattgc cttcattttc atgcaggcag
    21001 atttccatca ctttcagcag gttaaacatc gcgatgtcat gcaagccgta ggttctgccg
    21061 ggaatagata attccacaca gcccactacg gaatagtcgc gcgcatcttc cagcgaaacg
    21121 ccacggttga ggaacgctgg caccaccact tcatcgttaa agatttgcgg aataccggtg
    21181 ccgaggcgaa tggtttcggc ggttttcatc aggaacggcg tgtcgatcaa ggcgttagtg
    21241 cgcacgccga ggttcggttg cggtaattgc acgctttgat aggcgtcaag gcaaaggaac
    21301 gaaagcacgt tcaccgcgct acgtccgttc tcggttaacc cgccgagcag tgcggtatag
    21361 ccggtcggga aaccggcgaa ataacgcgcg ctgctggtgg agcgcaacag cacgatgtcg
    21421 ttgcatttca cccataaaga ttcgagcagt tctttcagga acgccgcatc ttcgccctgg
    21481 gttaatgatg tctgatagaa cggcaacata tactggtcga agcgccccaa cgatagcgaa
    21541 ctggcgttgg attcgtattg cagaatgatg ttcatgtacc agaataactg gcacgcctgc
    21601 cagaacgtct gcggcttatg ttgcgcgtta tggcgggaga tctccgcaat agtcagcagc
    21661 tcttcgcgac gctgggcatc tgtgcagttt gccgccatcg tttccgccag ttcggcgtaa
    21721 cgcaaaatgt gtttctgcga ggcttccagc agtaacagcg ctgcctgata aaagtgattc
    21781 tccggctgtt gctgacaatg ttgctgcatc tgtgctacca gctcccccag cccgtgattc
    21841 agcaggcgtg ggtaatcaat aataatgtgc ccctggcctt tatctgtctg gttgatgctg
    21901 aaaatctgcg tgctggtcgc ggcttttact tcatctgtca tctgcccgtt gatgaaatct
    21961 ttcatcgaac gtttttccca gtacgggaac aactcttcgc ggtagatacg tttatcttct
    22021 tcgctgatag caaagcggtc ctgcggacgc gtcgggaatt gatccagctc tttcagcagc
    22081 cagtaagggt ccatttccgg cgacataatc ccggcgcgcg gttttacggt gcggttaccg
    22141 gcaatcagtt cttcatcacg aatcgaaatt tcaacatgtt caaggatata cgctgttgct
    22201 ttcgcccggc gcaatatcac cggttcgcct tcggtttgcc gatggctggc ggtataaagc
    22261 agcgcccgct ccagcgagat ttcacgggta ttggcaaaca gtgcagtttt gaggcgagag
    22321 atacgattcg tcatgaggaa ctcctgtggg aaagcgaacc cgcccttacg ggcgggataa
    22381 gaacttaagc ggtttgcgcc aggtgagctt caattttgct cataatggca tcagcacgtt
    22441 ttaccgcatc gctgatgttg acgcgcacga tggtcttgcc agcaaaacgc tcttcaaact
    22501 tgatgccgat atctttagtc agaatcacca tatcggcgct cgccacgtct tccgcagtca
    22561 gttcattttc cagaccaatt gagccctggg tttctacttt cacttcccag cctttcgctt
    22621 tcgcggcact ttccagcgct tctgctgcca tataggtatg ggcaacacct gaaggacatg
    22681 cggttactgc aataattttc gtcatggctt gttcctcaat taattaattt caaaatccaa
    22741 atccaggtcg tcttcttttt catcagtcga agacccattt ttacgcgcca gacttttcag
    22801 cacgttcaca caaacagccg tcaccactgc tccgacagcc actgcggcga tataacccag
    22861 cttgccttca accaccggca gtacaatcag accaccccag cctgcatagc actgcgcacc
    22921 gaccagcgcc gcagttatcg caccacaaac agaaccaacc atgatggaag gaatgacacg
    22981 cagcggatcg gcagcggcga aaggaatcgc cccttcagta accccaacgc accccatcac
    23041 cagcgccgct ttaccagttt cgcgctcttc tgcggagaaa tttttacgac caatcagcgt
    23101 cgccagcccc atccccagcg gtggaacaca aatcccaaca gcggcaatag ccaccacggt
    23161 ataaacaccc tgagcaacgc aaatcagcat gaaggcatag gccactttgt taaccggacc
    23221 gcccatatcg aacgccagca tcagacccat gatcaccgcc agcataacaa tgctgccctg
    23281 ctgcatcccc tgaagccact gagtcaggct gttggtcaac gcccctacag gctcgcccaa
    23341 gccccacatc atgatgcctg cggtaatcag tgtgccgacg ataggaatga tgaagatagg
    23401 catcaccgag cgcagaactt tatgcaccgg aattttcttc aggtaatgca ccacgatgcc
    23461 gccgataatc ccggcgatca gcgcaccaaa gaagcccgca ccaaagctgt taccaaccca
    23521 ggcaccgata gcgcacggag ccagcgcaga acgctctgca atggagtaac caatgtaagc
    23581 ggcgaggaaa ggcaccatca gcgtcaaacc cgcaacgccg atatcaaaca gttttttcag
    23641 attcggatcg gctacggcat ccggcactgc gcctttgcca tacaacatga cggaaaccgc
    23701 cagcaaaata ccgcccgata ccacgaaggg aatcatgtgt gaaacgcccg tcattaaatg
    23761 ctgacgggta tttttcagga tctgcaccaa ctcattcata agctgctccc gggcttgcaa
    23821 aagcccatgt ccatattttt gatggaggaa acaataggca atcgcaggcg gagcaaacag
    23881 tggataaaaa ggccatttac tggacttttg taatatcagt acaaaaatgc gatccgcctc
    23941 ataacttgcg ataatccgga agcatgccgc aaaacggcgt cttgtgagac gcgtcgcaaa
    24001 ttgacggtta cattacattt gtccagcttt tggcagattt gtcacatggc aggccgttgc
    24061 cgtgcgctct tatcctgtaa acagaaaacc gtaccgggag agaggcaatg gccctgattg
    24121 tggaatttat ttgtgagcta cctaacggcg tacatgcacg tccggcaagc cacgttgaaa
    24181 cgctgtgtaa tactttttca tcacaaattg agtggcataa cctgcgcact gaccgtaaag
    24241 gcaacgccaa aagcgccctg gcgctgattg gcaccgacac gctggtgggc gataattgcc
    24301 agttactgat ttccggggcc gacgaacagg aagcgcacca gcgtttaagc caatggctgc
    24361 gcgatgaatt cccccactgc gatgcgccgc tggcggaagt taaatctgac gaactggaac
    24421 cactgccggt ttcattgacc aatctgaatc cgcaaattat ccgcgcccgc accgtgtgca
    24481 gcggtagcgc aggcggcatt ctgacgccga tctcttcttt agatctcaat gcgctgagta
    24541 atcttcccgc tgccaaaggc gttgacgccg aacaatccgc actggaaaac ggcctgacgc
    24601 tggtactgaa aaacattgag tttcgtctgc tggatagcga cggtgctacc agcgcgattc
    24661 tggaagcgca ccgatccctg gctggcgata cttccctgcg cgaacattta ctggcaggcg
    24721 tcagcgccgg attaagctgc gccgaagcga ttgtcgccag tgcgaatcac ttttgcgaag
    24781 agttcgcccg ttccagcagc agctacctgc aagaacgtgc cctggacgta cgcgacgtct
    24841 gcttccagtt actccagcaa atctacggtg agcaacgctt cccggcaccg ggcaaactga
    24901 cgcagcccgc catttgtatg gctgatgaac tgactcccag ccagttcctc gaactggata
    24961 aaaatcacct caaaggtctg ttactgaaaa gtggcggcac cacctcacat accgtaattc
    25021 ttgcccgttc gttcaacatt ccgacactgg ttggtgtgga tattgatgcc ctcactccgt
    25081 ggcagcatca aacgatttat atcgacggca acgctggggc gattgtggtt gagccagggg
    25141 aagccgtagc tcgttattat cagcaagaag cccgcgtaca ggacgccctt cgtgagcaac
    25201 agcgtgtctg gctgacccaa caagcccgta ccgctgacgg tatccgtatt gaaattgccg
    25261 ctaacatcgc tcactccgtg gaagcgcagg ccgcattcgg caatggtgcg gaaggcgttg
    25321 gtttgttccg cactgaaatg ctctatatgg atcgcaccag tgcaccgggt gaaagcgagc
    25381 tgtacaacat tttttgtcag gcgctggagt ctgccaacgg acgcagcatt attgtgcgca
    25441 ctatggacat tggcggtgat aaacccgttg attatctgaa cattccggca gaagctaacc
    25501 cgttcctcgg ttatcgcgcg gtgcgtattt atgaagaata cgcgtcgttg tttaccacac
    25561 aactacggtc aatcctgcgc gcctctgctc acggcagcct gaaaatcatg atcccgatga
    25621 tctcctcaat ggaagagatc ttatgggtga aagaaaaact ggcggaagcc aaacagcaac
    25681 tgcgtaacga acacattccg tttgatgaga agatccagct cggcattatg ctggaagtgc
    25741 cgtcggtgat gttcatcatc gatcaatgct gcgaagagat tgatttcttt agtattggta
    25801 gtaatgacct gacgcaatat ctgctggcgg tggatcgcga taacgctaag gttactcgtc
    25861 actacaacag cctgaatccg gcattcttgc gggcgctcga ttacgccgtg caggcggtgc
    25921 atcgccaggg caaatggatt ggtctgtgcg gtgagctggg agcgaaaggt tccgtgctgc
    25981 cgttgctggt cggtttaggg ctggatgaac tcagcatgag cgcaccgtca attccggcgg
    26041 cgaaagcgcg aatggcgcaa cttgatagcc gtgagtgccg ccagttgctc aaccaggcaa
    26101 tggcctgccg tacttcgctg gaagtggaac atctgctggc gcaattccgc atgacccaac
    26161 aggacgcacc gctggtcacc gccgagtgca tcacgctgga aagtgactgg cgcagcaaag
    26221 aagaagtgct caaaggcatg accgataacc tgctgctggc gggccgctgc cgctatccgc
    26281 gtaaactgga agccgactta tgggcgcgcg aggccgtttt ctctaccggt ctgggcttta
    26341 gttttgccat tccccacagc aaatcagaac acattgagca atccaccatc agcgtagcgc
    26401 gtctgcaagc gccggtgcgc tggggcgatg atgaagcgca attcatcatt atgttaaccc
    26461 tgaacaaaca cgctgcgggc gatcagcata tgcgcatttt ctcgcgcctc gctcgccgca
    26521 tcatgcacga agaattccgt aacgcgctgg ttaacgccgc ctctgccgac gctatcgcca
    26581 gcctgctgca acatgaactg gaactgtaaa aggaaacatc atggaactgt atctggacac
    26641 cgctaacgtc gcagaagtcg aacgtctggc acgcatattc cccattgccg gggtgacaac
    26701 taacccgagc attatcgctg ccagcaagga gtccatctgg gaagtgctgc cgcgtctgca
    26761 aaaagcgatt ggtgatgagg gcattctgtt tgctcagact atgagtcgtg acgcgcaggg
    26821 tatggtggaa gaagcgaagc gcctgcgcga cgccattccg ggcattgtgg tgaaaatccc
    26881 ggtgacttcc gaaggtctgg ccgccattaa gttgctgaaa aaagagggta ttactacact
    26941 tggcaccgct gtatatagcg ccgcacaagg gttattagcc gcactggcag gggcaaaata
    27001 cgttgctccg tatgttaacc gcgtagatgc ccagggcggg gacggcattc gtacggttca
    27061 ggagctgcaa acgctgttag aaatgcacgc gccagaaagc atggtgctgg cggccagctt
    27121 taaaacgccg cgtcaggcgc tggactgttt actggcagga tgtgaatcca tcaccctgcc
    27181 cttagatgta gcgcaacaaa tgctcaacac ccctgcggta gagtcagcta tagagaagtt
    27241 cgaacacgac tggaatgccg catttggcac tactcatctc taaaggagca attatggacc
    27301 gcattattca atcaccgggt aaatacatcc agggcgctga tgtgattaat cgtctgggcg
    27361 aatacctgaa gccgctggca gaacgctggt tagtggtggg tgacaaattt gttttaggtt
    27421 ttgctcaatc cactgtcgag aaaagcttta aagatgctgg actggtagta gaaattgcgc
    27481 cgtttggcgg tgaatgttcg caaaatgaga tcgaccgtct gcgtggcatc gcggagactg
    27541 cgcagtgtgg cgcaattctc ggtatcggtg gcggaaaaac cctcgatact gccaaagcac
    27601 tggcacattt catgggtgtt ccggtagcga tcgcaccgac tatcgcctct accgatgcac
    27661 cgtgcagcgc attgtctgtt atctacaccg atgagggtga gtttgaccgc tatctgctgt
    27721 tgccaaataa cccgaatatg gtcattgtcg acaccaaaat cgtcgctggc gcacctgcac
    27781 gtctgttagc ggcgggtatc ggcgatgcgc tggcaacctg gtttgaagca cgcgcctgtt
    27841 cccgtagcgg cgctaccact atggcgggcg gcaagtgtac ccaggcggcg ctggcactgg
    27901 cagaactgtg ctacaacact ctgctggaag aaggcgaaaa agcgatgctt gctgccgaac
    27961 agcatgtagt gactccggcg ctggagcgcg tgattgaagc gaacacctat ttaagcggtg
    28021 taggctttga aagtggtggt ctggctgcgg cgcacgcagt gcataacggc ctgaccgcta
    28081 tcccggacgc gcatcactat tatcacggtg aaaaagtggc atttggtacg ctgacgcagc
    28141 tggttctgga aaacgcaccg gttgaggaaa tcgaaaccgt agctgcactt agccatgcgg
    28201 taggtttgcc aataactctc gctcaactgg atattaaaga agatgtcccg gcgaaaatgc
    28261 gaattgtggc agaagcggca tgtgcagaag gtgaaaccat ccacaacatg cctggcggcg
    28321 cgacgccaga tcaggtttac gccgctctgc tggtagccga ccagtacggt cagcgtttcc
    28381 tgcaagagtg ggaataacct actccaaact cccggcttgt ccgggagttt gaacgcaaaa
    28441 ttgcctgatg cgctacgctt atcaggccta cgcaatctct gcaatatatt gaatttgcgt
    28501 gcttttgtag gccggataag gcgttcactc cgcatccggc actctgtgcc agtaatctga
    28561 aactcccacg ttgttcagga attttttatc cgcttctgga cattttctct acagaacaat
    28621 cgttagcccg gaaggtcgaa tctgtgactc ttattttcac ggacgaacca gatgaaagct
    28681 tcactggcgc tgctcagcct gctaacggca tttaccagcc actccttaaa atctcccgcc
    28741 gttccaccaa ccgtggtaca aatccaggcc aataccaacc ttgccatcgc agacggtgcc
    28801 agacagcaaa ttggcagcac gctattttac gatccggcgt atgtgcagct tacttatcct
    28861 ggcggtgatg ttccgcaaga acgcggtgta tgttccgatg tggtgatccg cgcattgcgc
    28921 agccagaagg tcgatttgca gaaactggtt cactaagata tggcgaagaa ttttgctgag
    28981 tacccgcaaa aatggaagtt aaagcgcccg gacagcaaca tcgatcaccg tcgggtgcct
    29041 aatctggaaa cctggtttag ccgccatgat aaaacacgcc ccaccagcaa gaaccccagt
    29101 gactatcaag cgggcgatat tgtctcctgg cgactggata acgggctggc acatattggg
    29161 gtggtttcag atggcttcgc ccgcgacggt acgccgctgg tgatacataa cattggcgca
    29221 ggcgcgcagg aagaagatgt gctgtttaac tggcggatgg tggggcatta tcgttatttt
    29281 gttaaataag agatcgcccc acatgaagaa aaaattcaga tcctttttac actgacggct
    29341 tcttttttct tacggctcac cagcgccagt gccagcacaa tcagcacaat accgctaccc
    29401 tcaaccgcgc caggattctc accgagcagc caccaggaaa agagtacgcc gcaaacggga
    29461 acggccagtg tgcttaagct ggcaatactg gcaggcaagt ttttcaatac aaacaaccat
    29521 aagctccacg ccagcgccgt cgccagaatc gcactgtagg ccagcgccca gaacacggtg
    29581 ggttgccagt caatttcacg ttgcggtacc agtaaagcga ccacactcat caccagcgcc
    29641 gcatacagca tctgccagga tgttaacgac aataaatcca cgcgcggatg acgggcgtaa
    29701 aggcgtttag cgacaatggc gctcgccccc caactgacac cggagaggat cgccagcatg
    29761 gcgcttttca tcgaagagaa atcgagttgc cacggctgca acaccaaaaa taaaccgaaa
    29821 gcggcaatca gaatcgcgaa atattgccca cgtcgcaggc gttcaccgag aaacaacgcg
    29881 gcgaaaatca ctacccagaa cggcatggta tagctcagga tcgctacttt ccccgctccg
    29941 ccgctgacca gcgcccactg cgccagacca accatcccac aggtttgtaa cagggcaatg
    30001 actaaggtgt aattaaacgg cgtcgggcgc attccgcgac cacgtaataa aaggacgatg
    30061 aataaaacga gagcgccgaa aatgcagcgt aaggcggtga agtcgaaggc accgatgtag
    30121 ctggtgactt gcttcatgaa aatccagcta taactccaga taagtgtgag cacaaccagg
    30181 ccactgattg ccagtgggtt gctctttcct gcggcagaca tgagagatcc agtgtgtagt
    30241 tttcctgtgg acactatacg ccggtaaaga aacttcagcg actgcctgaa aggcagtcgc
    30301 tgaagaaggt cagattacag catgtcgaaa cggtcgaggt tcatcacttt cacccatgcc
    30361 gccacgaagt ctttaacaaa cttctcgtgg gcatcgctgc tggcgtaaac ttcagccacc
    30421 gcacgcagga ctgagttaga accaaataca agatccgcac ggctggctgt gtatttcact
    30481 tcgccagttt cacggtcacg gccttcgaac agctctttcg attcgtccgc cgctttccac
    30541 tcgtaacgca tatccagcaa attcacgaag aagtcattgc tcaatacgcc aacgcggtca
    30601 gtgaagacgc cgtttttgct gccatcgaag ttggcaccca gtacgcgcat cccgcccacc
    30661 agcgcagtca tttccggcgc ggtcagcgtc agttgctggg ctttgtcgat cagcagtgac
    30721 tcggtggtgg aaacatccag acgagcgcga tagttacgga aaccgtcagc aattggctcc
    30781 agcagttcga acatctcaat gtcagtctga tcctgacgcg catcaacgcg acccggcgca
    30841 aacggtacat gaatgctcaa acctgcggcg cttgcggctt tctcaacacc aaccacacca
    30901 gccagcacga tgatatccgc cagcgaggct ttaccagact ctttctggat tttctccaga
    30961 acaggcagag cacgaacggc tgcggcgttc acatcccagt cgcgctgcgg cattaatgcc
    31021 agacgcgcac cgttggcacc accgcgtttg tcgccaccac ggaaggtaga agcagatgcc
    31081 caggccaccg ataccagctc actaacagac agaccagaat ccgcaatcgc gaatttcaga
    31141 tcgataatgt cctgctcggt cgggttgtag atcggctgcg gcagcggatc ttgccagatc
    31201 agatcttctt tcggcacttc cggcccgatg tagcgagatt tcggccccat atccctgtgc
    31261 gtcagtttga accaggcacg ggcaaaggct tcgttgaacg cctgcggatc gttgaggaaa
    31321 cgacgagaga tcttctcgaa ctcaggatca aaacgcagcg tcaggtcggt caccagcatt
    31381 gtcggtttac gtttcttcga cggatcaaac ggatccggga taatttccgg tgcgtctacc
    31441 gcttcgaact ggattgcgcc agccgggctg cgggtctgta cccactcata cttgaacagg
    31501 ttctcgaaga aatagttgct ccactgggtc ggcgtctggg tccagactac ttccagacca
    31561 gaggtaatgg catctgcgcc aacgccgctg ccgtaagtgc tcgcccaacc taaaccttgt
    31621 tcttcaatcg gtgcagcttc tggatcagga cctacatttg atgtcggacc ggcaccgtgg
    31681 gttttaccca gcgtatgacc acccgcaatc agcgccacgg tttcttcgtc gttcatgccc
    31741 atgttgccga aggtcgcgcg gatagctgct gccgcagaaa gcggttcgcc gctgtgatcc
    31801 gggccttccg ggttaacgta aatcagaccc atctcggttg cacccagcgg tgctttcgcc
    31861 agcgcttccg gatgacggtg agtcagccag gctttttcat caccccagtt aacatccaga
    31921 tccggttccc agacgtcttc acgaccggca ccaaaaccga aggtacggaa gccggagttt
    31981 tctagcgcca cgttacccgc gaggataaac aggtcggccc aggagatttt ctgaccatat
    32041 ttctgtttga ttggccacaa caggcgacgc gctttatcga ggcttacgtt atccggccag
    32101 gagttcagcg gtgcaaaacg ttgctgacca cgacccgcgc caccgcgtcc atcgattgaa
    32161 cggtaagtcc ccgcgccgtg ccaggccata cgaataaaca gaccggcgta actgccccag
    32221 tcggctggcc accacggttg agattctgtc aacagggctt tcagatcttt tttcaggccg
    32281 tagtaatcta atttgctgaa ttctttgcgg tagtcaaagt cctcacccag tgggttagaa
    32341 cgattagaat gttggtttaa caggtcaaca cgaagttgat ttggccacca gtcgcgagtg
    32401 gttgtgcccg cccccgcact ctggtcgtga ccgccctgat ggaacgggca tttgccagtg
    32461 gctgtggtgt tatggatatc gtctgacgtg ctcatcaatg tgctcccctc tacagtgtta
    32521 ccgttacgat acacagcgtt agagagaagt tataattgaa ttaatccacg gatgcgatag
    32581 ttgagatctt acatattgtt ggttaaagag atgtagatca aattgatctt aattaagctt
    32641 ttttgattat gtccgaattc ggacaaatgc tttataaaaa agggttttag cttcatatcc
    32701 ttcaagtaat gagggagaaa acaaggctga taaccttatt ttcccgccct catttcgagg
    32761 cagcattttg tgctctgttt aaaatttgtg atcactgtgt gattttcaca aaagccacac
    32821 tatttataaa ccaggtcgaa cccccagcgt atggcaaatc gcgtaactca tttcagcacg
    32881 gttaagcgta tagaagtgga aatctttcac tccttcacgg cttaaaatct tcaccatatc
    32941 catggcaata ttcgcgccaa ccagtttgcg ggtttcggca tcatcatcca gaccgtcgaa
    33001 catttgcgcc atccacgccg gaatacgcac gttggtcata tcggcaaatt tcttcgcctg
    33061 tttaaagtta gataccggca aaattcccgg aataatttcc acatcaatgc ccgccgatac
    33121 acagcggtca cgaaaacgca ggtagctttc gacatcgaag aagaactgag taatcgcgcg
    33181 gttggctccg gcatccactt tgcgtttcag attaagcaaa tccgcctgag cgctttttgc
    33241 ttccgggtga acttccggat acgccgccac ggagatatcg aaatctgcca cttcttttaa
    33301 cagcgtcacc aggtcagaag catacatttc tggcttacca cttcccggcg gcagatcgcc
    33361 acgcagcgcc acgatatgac gaataccgtt attccagtag tcgcgtgcaa tggtgcgcag
    33421 ctcgtcgggc gtcgcatcaa tgcaagtaag atgcggtgcc gcttccagac cagtgcgatc
    33481 tttaatgcct ttaataatgc tgtgcgtacg gtcgcgctcg ccggagttcg cgccataggt
    33541 caccgataca aacttcggtt tcaggctgct aaggcgatcg atggagttcc acagggtctg
    33601 ctccatttca ctggtacgcg gcgggaaaaa ctcgaacgaa acgttaatct gcccctggac
    33661 ttctgccagg ctctgattca gggcatcccg ctggctggcg tgaaaaaagc tcatacctta
    33721 cctcatcaat cgcttgtagt tatatgttgt gttgtgcaca tctatccgtt tagacgtcca
    33781 gatgtaaaga tgaaggaaaa gccgaagggc gtcaaccgaa aaatgaccaa tgatggtgag
    33841 gaaaaatcag ggaagatgaa aaaacttcag gatggtaaga aaaagaaaat ccggtatgca
    33901 aagcaaaccg gactgcaaat ataaactaac catgtactta ttgcttcttg ccggattcgt
    33961 cgtaaacgct taatccggcc taatcctatg ataattcaat atatttcatt agaaaccata
    34021 aacccgaaaa catgagtacc gggcattatt aaatttctga aattacaaca actgtgccag
    34081 ccggttgata tccgactgaa tcgccccggc ggtgacgtcg cgccccgcgc caggcccacg
    34141 gatcaccagc gggttgtcgc gataccagcg gctttcgatg gcaaagacgt tatcgcacgg
    34201 cagcagtgat gccagcggat ggtcttcacg caccgcctcc acgcccacac gcgctttgcc
    34261 gttggcatcg aaacgcgcca cgtagcgtag caccagcccc atttcgcggg ccgcttccag
    34321 ccgttgcacc atctgctcgt taagttcatc gccattttca aagaaatggt cgatgctgcc
    34381 gccttcgcaa tgagcaggca ccagcgattc cacacgtacc tggtccggtt caatgttgta
    34441 acccgcttca cgcgccagaa ttaccagctt gcgcattacg tctttgccgg agaggtcgtc
    34501 acgcggatca ggctcggtta agccctgctg ccacgcctga tccaccagct cggtaaacgg
    34561 cacgctaccg tcgaattgca ggaacagcca ggagagcgtg ccggagaaga tcccgctgat
    34621 cgacaaaata gtatcgccgc tgtcgatcag atcgcgcacg gtgtggttga tcggcaagcc
    34681 cgcaccgacg gtggaattgt acagccagtg acgcccggtt ttttcgaagg cgtcgtggat
    34741 ctggcgatat ttattgctgt cgctggctcc cgccagtttg ttggcgctga taacgtggaa
    34801 accgtggctg gcgaaatcaa gatactgatc agcaagctgc tggctggcgg taacgtccag
    34861 caccactaaa tcatcatacg gatgggcgcg catccacagg aacaacgact cttcatcctg
    34921 ctcaaccgct tcatcgttga agaaggctaa cgcgcggctg gcgtccagcc cgtcatagct
    34981 caacaggctg cggcggctgt ccaccacacc tgccagcaca aactcaaagc cggtacgtgc
    35041 cgaaagcgtg ctctgctcac gggcgaacag ttccagccaa cgggaaccga tattgccctt
    35101 accgaacaat accaggccga tgcgtttttc tgcgcggaag acggactgat gcagcccctg
    35161 aatcaggctt tcggtcgggc cggtgcgcag tactgccacc aggctgatgc cgtcatcgga
    35221 ctgccaggta aattcgaccg gctggccttt cagttgctgc cagaagcggt ggcaatgcag
    35281 cgggttacgg gtgacgcctg caccgaccat cgccaccagc gccagcccct gacgcaggcg
    35341 cagttcgcca ggtaatcccg cttcgtcgag gattttcagc gcactgtcgg ccacttctga
    35401 ggtgtagcaa aattgcagca actggcgatc gttatgtacg ccaaccgcca gcgggcgtac
    35461 ctgcgcgcgt ttcaggattt ggtcgatctc tttatgcgcc agtttgaaat cctgactggc
    35521 gggcacctga aactcaatca aacagacatc atcgtggctg gtgacaatac gcgcaccagt
    35581 accggaggcc agcacgcgtt caatgcgcgt ggaaccttga tccggcgtgt agctacagcg
    35641 cagttgcagg tcgatttcgc tgccagaaac cggctgtaaa gtacgggcgt gaagaacggg
    35701 agccgccagg cgcgccagtt cgctggcctc atccagacgc agcaacggca gcaggcaggc
    35761 atctttcact ttacgcgggt cggcactgta taccccggcg acgtcgctcc agatggttac
    35821 gcgagaaaca cccgccagcg caccgatttg tgtcgcggaa tagtcggaac cgttacgccc
    35881 cagcagcacc gtttcaccgg cgttgttgcg gctgataaat ccggtcacca ccagacgttt
    35941 gcccggatgt tgcaccagca gctgttgcag caacgggtaa gaaagccctt catcaacctg
    36001 cggttgtgcg gcgcgttcag cgcgtaaaaa ctcgcgggca tcaagccagg ccgctggcag
    36061 cccttgttga ttaagtaccg cagacatcag gcgtgccgac catacttccc cgtggcccac
    36121 cacttccgca tacactgcgt cgttaatacc gctgtcgagc agcgccgcca ggcgctcaag
    36181 gtcgctgaca aaagcgctaa tgaggctatc ggcttcttca gcgggtagca gaccgctaat
    36241 cagatcacac tgatagcgac gcagcgtttg ttgaacctga tgcgcagaga gacgatcggt
    36301 ctggcttagt ttcaaccagt taatcaactg gttagtggtg ctaccggcag cggaaaccac
    36361 catcatatcg tcaggctgag agtactccgc cataatgccc gcgacacgca aataacactt
    36421 cacatcagcc agactactgc caccaaattt atgcagctga cgacctttcg cccctgcctg
    36481 cgcaatcaca ctcattttta ccccttgttt gcagcccgga agccattttc caggtcggca
    36541 attaaatctt cgccatcttc aataccggtg gagatacgca gcagcgtctc ggagatcccg
    36601 gcggcagcac gcgcttctgg tgccatgcct gcatgtgtca tggttgcggc gtgagagatt
    36661 aaactttcca ctccccctaa tgattccgcc agcgtaaaca acgacagccc gcccaggaaa
    36721 cgacgcagcg tctgctcatc gccatccagt tcaaaactca acattgcgcc aaagcctttt
    36781 tgctggcgcg cggcaatttc atgcccctga ttttccggca gcgacgggtg atacagtttt
    36841 ttcaccaacg gctgggtttg cagatatttc acaatcgcct gcgcgttgcg ctgggccaac
    36901 tccatgcgcg gcaccagcgt tcgcaacccg cgtagcagca gatagctgtc aaacgcgccg
    36961 cccgtcacgc caatattgtt tgcccaccag gcaagttcag tgacaacgtc cgggtcttta
    37021 gcaatcacca cgccagccac cacgtcagag tgaccgttca gatatttcgt gcatgaatgc
    37081 aacaccagat cggcacctaa tgccagcgga ttttgtaatg ccgggcttaa gaaggtgtta
    37141 tccaccacgc tcaccgcccc gacttcccta gccagatggc agattttcgc aatatccacg
    37201 acgcgtaaca atggattact tgggctttct accagtacca gtttgggttt ttctgccagc
    37261 gctgcccgta atgcctgttc atcgccttga tcaacaaaca acacgcgata gcaaccgcgt
    37321 ttcgccaggc tgtcgaacag gcgatagcta ccgccgtagc agtcgtgcgg cgcaaccagc
    37381 agatcgcctg gtttcaaaaa gacggtcgtt accaggtgaa tcgcggacat gccggtatta
    37441 gtaagtactg caccagcacc gccttccagt tctgccagcg cacgctgaac cacatcgcgc
    37501 gttgggttgc cgcgacgcga gtaatcatgc gcgcgcggtt cattaaatcc ggtaaagtta
    37561 taggtactgg aaagatggat cggtgggaca acgcaaccat actgttcgtc gtcatttaac
    37621 ccgctacgca ctgcgatggt ggcctgttta cgcgtcatgt gatgaagttc cctgggcttg
    37681 gtcggtgaaa tgtcaggcac cagagtaaac attgtgttaa tggacgtcaa tacatctgga
    37741 catctaaact tctttgcgta tagattgagc aaatcccaaa tagccgttaa aattatatgc
    37801 attatcacgc cgacaggtgc attacacgat gtcacggtaa cgcctgtacg gtaaactatg
    37861 cgggtttacg gtcagtaccc acataaattg tgtggtctgg tctcaattta ttgacgaaga
    37921 ggattaagta tctcatggct gaatggagcg gcgaatatat cagcccatac gctgagcacg
    37981 gcaagaagag tgaacaagtc aaaaagatta cggtttccat tcctcttaag gtgttaaaaa
    38041 tcctcaccga tgaacgcacg cgtcgtcagg tgaacaacct gcgtcacgct accaacagcg
    38101 agctgctgtg cgaagcgttt ctgcatgcct ttaccgggca acctttgccg gatgatgccg
    38161 atctgcgtaa agagcgcagc gacgaaatcc cggaagcggc aaaagagatc atgcgtgaga
    38221 tggggattaa cccggagacg tgggaatact aaagcaaaaa agagcggcgc ggagtggaat
    38281 cgcctgatgc gctacgctta tcaggcctac gtcatattgc aatttattga atttgcacga
    38341 acttgtaggc cggataaggc gttcacgccg catccggcat aaacaacgag cacgttgtct
    38401 gcgacccacc gctttttata catggacgtt taactatgaa aaacaggctg ctgatcctca
    38461 gcctgctggt ttctgtacct gcctttgcct ggcagccaca aaccggcgac atcatctttc
    38521 agatctctcg ctcatcgcaa agtaaagcga tccaactggc gacccattcc gattatagcc
    38581 acaccggtat gctggtgata cgcaacaaaa agccctacgt ttttgaagcg gtcggcccgg
    38641 tgaaatacac cccgctcaag cagtggatcg cccatggtga aaagggcaaa tacgttgttc
    38701 gccgcgttga
//
LOCUS       DAJOSY010000029        38412 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJOSY010000029 DAJOSY010000000
VERSION     DAJOSY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.029"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            87..1745
                     /locus_tag="OHN27_001653"
     CDS             87..1745
                     /locus_tag="OHN27_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCQ2286109.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1742..2698)
                     /locus_tag="OHN27_001654"
     CDS             complement(1742..2698)
                     /locus_tag="OHN27_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCQ2286110.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2849..3964
                     /gene="macA"
                     /locus_tag="OHN27_001655"
     CDS             2849..3964
                     /gene="macA"
                     /locus_tag="OHN27_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCQ2286111.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3961..5907
                     /gene="macB"
                     /locus_tag="OHN27_001656"
     CDS             3961..5907
                     /gene="macB"
                     /locus_tag="OHN27_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCQ2286112.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5980..6204)
                     /gene="cspD"
                     /locus_tag="OHN27_001657"
     CDS             complement(5980..6204)
                     /gene="cspD"
                     /locus_tag="OHN27_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCQ2286113.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6527..6847
                     /gene="clpS"
                     /locus_tag="OHN27_001658"
     CDS             6527..6847
                     /gene="clpS"
                     /locus_tag="OHN27_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCQ2286114.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6878..9154
                     /gene="clpA"
                     /locus_tag="OHN27_001659"
     CDS             6878..9154
                     /gene="clpA"
                     /locus_tag="OHN27_001659"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCQ2286115.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKAKN
                     ELTYGFQSAQKHKAEAAH"
     repeat_region   9366..9570
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9366..9393
                     /rpt_unit_seq="gttcactgccgtacaggcagcttagaaa"
     gene            complement(9614..9701)
                     /locus_tag="OHN27_001660"
     tRNA            complement(9614..9701)
                     /locus_tag="OHN27_001660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9665..9667),aa:Ser,seq:gga)
     gene            complement(9955..10173)
                     /gene="infA"
                     /locus_tag="OHN27_001661"
     CDS             complement(9955..10173)
                     /gene="infA"
                     /locus_tag="OHN27_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCQ2286116.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10458..11162)
                     /gene="aat"
                     /locus_tag="OHN27_001662"
     CDS             complement(10458..11162)
                     /gene="aat"
                     /locus_tag="OHN27_001662"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCQ2286117.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11204..12925)
                     /gene="cydC"
                     /locus_tag="OHN27_001663"
     CDS             complement(11204..12925)
                     /gene="cydC"
                     /locus_tag="OHN27_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCQ2286118.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTTGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12926..14692)
                     /gene="cydD"
                     /locus_tag="OHN27_001664"
     CDS             complement(12926..14692)
                     /gene="cydD"
                     /locus_tag="OHN27_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCQ2286119.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14815..15780)
                     /gene="trxB"
                     /locus_tag="OHN27_001665"
     CDS             complement(14815..15780)
                     /gene="trxB"
                     /locus_tag="OHN27_001665"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCQ2286120.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHDHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16325..16819
                     /gene="lrp"
                     /locus_tag="OHN27_001666"
     CDS             16325..16819
                     /gene="lrp"
                     /locus_tag="OHN27_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCQ2286121.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16954..20943
                     /gene="ftsK"
                     /locus_tag="OHN27_001667"
     CDS             16954..20943
                     /gene="ftsK"
                     /locus_tag="OHN27_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCQ2286122.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQSQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVPPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQQQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPV
                     DTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLA
                     RSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDI
                     AGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELS
                     VYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRM
                     MRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKAR
                     AAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDM
                     LYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGA
                     EELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNR
                     EVLAPPPFD"
     gene            21098..21709
                     /gene="lolA"
                     /locus_tag="OHN27_001668"
     CDS             21098..21709
                     /gene="lolA"
                     /locus_tag="OHN27_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCQ2286123.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21720..23063
                     /gene="rarA"
                     /locus_tag="OHN27_001669"
                     /gene_synonym="mgsA"
     CDS             21720..23063
                     /gene="rarA"
                     /locus_tag="OHN27_001669"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCQ2286124.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23154..24446
                     /gene="serS"
                     /locus_tag="OHN27_001670"
     CDS             23154..24446
                     /gene="serS"
                     /locus_tag="OHN27_001670"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCQ2286125.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24685..27129
                     /gene="dmsA"
                     /locus_tag="OHN27_001671"
     CDS             24685..27129
                     /gene="dmsA"
                     /locus_tag="OHN27_001671"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCQ2286126.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27140..27757
                     /gene="dmsB"
                     /locus_tag="OHN27_001672"
     CDS             27140..27757
                     /gene="dmsB"
                     /locus_tag="OHN27_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCQ2286127.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27759..28622
                     /gene="dmsC"
                     /locus_tag="OHN27_001673"
     CDS             27759..28622
                     /gene="dmsC"
                     /locus_tag="OHN27_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCQ2286128.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAV
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28657..29283)
                     /locus_tag="OHN27_001674"
     CDS             complement(28657..29283)
                     /locus_tag="OHN27_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCQ2286129.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRLSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29597..30745
                     /locus_tag="OHN27_001675"
     CDS             29597..30745
                     /locus_tag="OHN27_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2286130.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30955..32385
                     /locus_tag="OHN27_001676"
     CDS             30955..32385
                     /locus_tag="OHN27_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2286131.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32386..33294)
                     /locus_tag="OHN27_001677"
     CDS             complement(32386..33294)
                     /locus_tag="OHN27_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2286132.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSCIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            33394..33984
                     /locus_tag="OHN27_001678"
     CDS             33394..33984
                     /locus_tag="OHN27_001678"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCQ2286133.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(34066..34806)
                     /gene="pflA"
                     /locus_tag="OHN27_001679"
     CDS             complement(34066..34806)
                     /gene="pflA"
                     /locus_tag="OHN27_001679"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCQ2286134.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34998..37280)
                     /gene="pflB"
                     /locus_tag="OHN27_001680"
     CDS             complement(34998..37280)
                     /gene="pflB"
                     /locus_tag="OHN27_001680"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCQ2286135.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(37335..38192)
                     /gene="focA"
                     /locus_tag="OHN27_001681"
     CDS             complement(37335..38192)
                     /gene="focA"
                     /locus_tag="OHN27_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCQ2286136.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9482 a         9339 c        10201 g         9390 t
ORIGIN      
        1 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
       61 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      121 gcggtatcaa ccgtttgtcg ttgatgctcg aacaaaacaa cgtcctgatt ggggagaacg
      181 cgtggggtaa atccagcttg ctggacgcct taactctgct gctatcgcca gaatcagatc
      241 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      301 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      361 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      421 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      481 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      541 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      601 cggtgccaaa tgtccctaat gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      661 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      721 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      781 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
      841 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
      901 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
      961 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1021 atcttctgcc attgcagcgc attgccacca ccaactcggg tgagttgctt tcgttaacgc
     1081 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1141 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1201 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1261 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1321 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1381 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1441 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1501 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1561 tcccggaaaa tgtaccgatg aatctgcgca aaattatctc gaaagcgatc catcgctctt
     1621 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1681 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1741 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1801 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     1861 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     1921 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     1981 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2041 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2101 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2161 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2221 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2281 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2341 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2401 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2461 gtcgcggctg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2521 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2581 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2641 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2701 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2761 tgtgattact aacgtaaatt ttagggtttt gttgatattt cgttgaagtt aatgacccgg
     2821 attggcatat ggagtattca gaaaatttat gaaaaagcgg aaaaccgtga agaagcgtta
     2881 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     2941 acccgtgccg acttatcaga cgctgattgt gcgccccggt gatttacagc aaagcgtgct
     3001 ggcgaccgga aagctggacg cgctgcgtaa ggttgatgtg ggcgcgcagg tcagcggtca
     3061 gttgaaaact ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3121 tattgatcct gaacaggctg aaaaccagat taaggaggtc gaagcaacgc tgatggagct
     3181 acgtgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3241 tcagcaacgt ctggcacaaa cgcaggctgt ttcacagcag gatctcgaca ccgccgcgac
     3301 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3361 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3421 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3481 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3541 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtgc ttggcgatcc
     3601 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3661 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3721 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3781 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     3841 tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     3901 caaagggctt gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     3961 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg tgatgagcag
     4021 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4081 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgtct ggataaggcc
     4141 accagcggca cctatcgcgt cgccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4201 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4261 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4321 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4381 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4441 aacggtggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4501 gaagaggtga tggcgatcct gcatcagctg cgcgatcgtg ggcatacggt gattatcgtc
     4561 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4621 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4681 gtcaacacgg tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4741 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4801 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     4861 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     4921 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     4981 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5041 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5101 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agcttaacgg tcgtgcgcag
     5161 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5221 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5281 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5341 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5401 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggaaagaag
     5461 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5521 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg tattggtgta
     5581 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5641 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5701 ctggtcggtg gcgcgttggg aataacactg tcactgttaa ttgctttcac cttgcagctt
     5761 ttcttacccg gctgggagat tggtttttca ccgttggcgc tgctgctggc gtttctctgc
     5821 tcgacggtca ccgggatttt atttggctgg ttacccgcac gaaatgcggc acgactggat
     5881 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     5941 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6001 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6061 aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg aataatgagc
     6121 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6181 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6241 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6301 tttagagaat tagggcgagc cgtcaagcat ttgacagggg acaaggggca ggtatgaatc
     6361 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6421 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6481 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6541 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6601 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6661 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6721 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6781 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     6841 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     6901 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     6961 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7021 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7081 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7141 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7201 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7261 agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag acgagccgac
     7321 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7381 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7441 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7501 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7561 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7621 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7681 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7741 caccattatc ggtgcgggtg cagcgtctgg tggtcaggtc gatgcggcta acctaatcaa
     7801 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     7861 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     7921 accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     7981 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8041 cattaacgat cgtcatctgc cggataaagc cattgatgtt atcgacgaag cgggcgctcg
     8101 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8161 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg atcgtgatac
     8221 cctgaaaaac ctcggcgatc gcttgaaaat gctggtcttc ggtcaggata aagccattga
     8281 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8341 tggttcgttc ctgtttgccg gccctaccgg ggtcgggaaa acagaggtga cggtacagct
     8401 ttcgaaagct ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8461 taccgtcagc cgtcttattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8521 gctgactgat gcggtcatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8581 agcgcacccg gacgtgttca atattctgtt gcaggtgatg gataacggta cgctgaccga
     8641 taacaacgga cgcaaagcag acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8701 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8761 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     8821 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     8881 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     8941 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9001 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9061 cggtcaggtc accgtcgcac tggataaagc gaaaaatgag ctgacttacg gattccagag
     9121 tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgtcaggtag gttggtcaag
     9181 tccgtaatct cgaaagaggt tacggacttt ttatttatgg ggtggaacgg tgaaaaacct
     9241 atttttggag gtgaaggtaa gttgttgata attagtgctg ctggaaggta aggataaaaa
     9301 agggtgctgc aggagaatgg gatggttttg ctttattaac aacgggctaa acgtgtagta
     9361 tttgagttca ctgccgtaca ggcagcttag aaaaacctac cgtcttggct agcggttgca
     9421 gcgaacgttc actgccgtac aggcagctta gaaaggaaca atcttgcaaa ggctgtgaaa
     9481 gttggcgttc actgccgtac aggcagctta gaaattcaca ggtaacatac tccacccacc
     9541 atgttcactg ccgtacagac agataaaatg cgaaaaaaaa gctcgcactt tcgtacgagc
     9601 tcttctttaa atatggcggt gaggggggga ttcgaacccc cgatacgttg ccgtatacac
     9661 actttccagg cgtgctcctt cagccactcg gacacctcac caaattgttt tgctgccaaa
     9721 cctcatgggt ggcaacgggg cgctactata gggagttgga gtaaaacggt caagaagaat
     9781 tttaatgata attattgttt gctcatactg taaacaactt gtgcagtata tctacatcga
     9841 gacaagttat ggacttatac ttccaaagta cttcatacat atcacaaaat aaaaaggccg
     9901 gttaaaccga ccttttactc gttctttctc ttcgcccatc aggcggtaaa acaatcagcg
     9961 actacggaag acaatgcggc ctttgctcag gtcgtacggg gtcagttcaa cagtcacttt
    10021 gtcgcccgtc aggatgcgga tgtagttttt gcgcatttta ccggagatgt gtgcagtaac
    10081 cacgtgaccg ttttctaact ctacgcggaa catggtatta ggcaacgttt caagaacggt
    10141 accttgcatt tcaatattgt cttctttggc catctaatcc tctggggtat cactaccgta
    10201 atttgaaccg gcaagataat gccgaagttc tgtaaataag taaagatttg cgcgctaaat
    10261 cgcaacaaac aggttcggca cattactccg aaaacacacg gctaagccgc accaaaagcg
    10321 caacgtataa gggagcggtg agataaacga tgggcgttac ctgacgcgaa aaattcctta
    10381 tcggcagcgg ggtaatgagc gtaaccaact ctgcgaccgc aattataaca ctctggggag
    10441 aaatgtgccg aaaacattca ttcttgtggt gaaaacaagc accgtggtac ccagaaatta
    10501 ttcggcaatc gtccgaggcg catttgattg agataattaa ggtaatcccg gcggggaatt
    10561 tcgcaggcac caagcgatgc tgtgtgatcg ttaaggacct ggcagtcgat aagcttaccg
    10621 ccatgaccga taaattcctc acagaatacc agaagcgccg ttttagacgc attttccatc
    10681 cggctgaaca tggactcgcc acaaaatagc gttccctggg ccacgccgta cataccgccg
    10741 acaagctcat cttcacgcca gacttcaatg gagtgggcat gaccgagttc gtgaaggcga
    10801 tggtaggctt cgaccacgcc acgcgtgatc caggttcctt cttcgcgatc gctggcacag
    10861 ccttcaatga cctgaccaaa agcgtaattc atcgtgacac gatagggcga gcgtttatga
    10921 aatcgcttca tactacggct gatatgcagt gattctggcc atagcaccgc gcggggatcg
    10981 ggcgaccacc agaggatggg gtcgcctgga gaaaaccacg gaaaaatacc acgctgataa
    11041 gccattaaca ggcgcgcagg gctaagatcg cccccaagtg ccagcaggcc gttaggctca
    11101 cgtaatgcgc cttccgggga agggaaggct attgaatggc gagaaagctg aaccaggcgc
    11161 atgaccgcaa aactccacgc aagtcggatc gttcaataat agcttacaaa ccctgcttga
    11221 actggtaata acgcccctgt ctggcaagca gttctgcgtg agtaccttgc tcaataattt
    11281 gcccgttgtc catcactatt atttgttgga aacgagagag tccgcgaagt cgatgggtga
    11341 ccattaacac cgttttctca cgcatcattt ctgcaagcaa ttcaaggatc tggctttcgg
    11401 ttgtggcatc taagccttct gtaggttcat ccagcaacac cagtggcgca tcatgtaaca
    11461 gcgcacgggc gatagccaga cggcgcagtt caccaccgga gagctggcgt ccgccttcac
    11521 ctaaccaact gttgagacct gcatcctcga gcagcttctc cagaccaacg cgacgcaaga
    11581 tctccgccag agcctcatca ctactgccag gcgaggcgag taacagatta tcacgcagcg
    11641 tggcgctaaa cagatgcact cgctgaggaa caacgctgat ggtctgtcgt agagccgctt
    11701 cattcaggct ggctatgggg ctatcgttaa gcaaaatctc gccctgttgc gggtcccatg
    11761 cgcgggtcag cagttgtaac agtgttgatt tgccgcatcc ggttcgcccg agaatcgcta
    11821 tatgttcccc ggcgtttacc tgaagagaaa tccctttaag tgcctgttga gattgctccg
    11881 gataagtgaa ctgaacatcc cgtaacgtca gcgaaacgcg atcggcaaca cgagtttggg
    11941 tatcagggaa ggtgacttcc ggtttttgat ccgttaaatc agtgatccga acggcagagg
    12001 caatgacttg ccccagatgc tgaaatgcac ccgttactgg tgccagtgct tcaaacgcgg
    12061 ctaacgcgca gaagacaaac agggcaatta acgcgccggg ttgagcattg ccgccaacgc
    12121 cgccagacgc catccacagc atcaggatca ccgctaacgc gccaatgagc agcattatcg
    12181 cttgcgacaa tgcggtcagt tcagattgac ggcgttgcgc ttccagccat tgaatttctg
    12241 tattctctaa ttgcgtgcga taacgatcgc tggcaccaaa aatggtcagc tcagcttgcc
    12301 cttgcagcca ggccgtcagt tgttggcgat actgcccgcg aagatgagtc agattttgcc
    12361 cggtgctttt tcccgcacga taaaacagcg gtggcatcag gaaaagcgtc agtaacataa
    12421 tgccgcccag cgtaaaggcg agggtgaaat caaggaaact taacccggtt gtcaccacca
    12481 taatcaccac aaaagcgccc accagcggcg agataacgcg caggtaaaga tgatcgagcg
    12541 tatcaacatc cgccaccacg cggttgagca attcgccctg acgatagcgc gccagtccgg
    12601 caggggagag gggcaacaat ttgctgaagg tgtaaatgcg cagatgctgc aacacgcgga
    12661 aagtcgcgtc gtgacttacc agacgttcaa aataacgccc ggcagtacgg gtgattgcgg
    12721 caccacgcac gcccgcagcg ggtagcatat agttgaagct gtacagtccg gcaacccccg
    12781 caaccgctga ggccgagagg aaccagccgg aaagtgtcaa cagaccgata ctggcgagca
    12841 gcgtcacaat tgccagcaca ataccaagac ttaacatcca tttatgacgt ttatacagtg
    12901 ccagataggg tagcaaagcg cgcatttaaa tctcctcctg acgatgggcc agtaatgtgg
    12961 cgaatgggcc accagccaca cttaattccg cgtaacgtcc ttgctcaata atccggccat
    13021 cctgcataac ccaaatgaca tcccagtcag caagatcttc taactggtgg gtgaccatta
    13081 acgttgtctg gcgcagagag gcggcattca gcgcctccat tacgcgctgt tcactgtgag
    13141 catcaaggct ggcagcgggt tcatccaaca gtaatagcga acagggattt agtaacgcac
    13201 gggccaccgc cacgcgctgc gcctgcccca cggaaaggcg ggcagcctgg tcgccaacag
    13261 gcgtatcaac gccttgtggc aggagcggta gaaactcgct gacccaggcg ttatccagcg
    13321 ctgcttgtaa ttcatgttcg ctggcatcag gtcgcgccag tagtacgtta tcccgcaatg
    13381 ttgctgccgg taattgtggg ttttgcccaa cccaggagag atgtttacgc catgattctg
    13441 gtgataaatc acgtaattct atcccgttga ttcgtaatga tccctgatat gagagaaaac
    13501 cagaaagcgc gttcagcagt gagcttttac ctgaaccgct gcgaccaacc aacactgcac
    13561 gttggcctgc tggcaaagta aagttcagcg gtccggccag cgttttacct tccggcgacg
    13621 tgataaacag atcctcggcc tcaatggtca gcggatcggt caatgctaat tccgcctcac
    13681 cgcgctgcgg atgggcgagc ggggtttcca taaacgtttt cagactgtca gctgcaccaa
    13741 cagcctgggc tttagcatga taaaacgtac cgagatcgcg taatggctgg aaaaactctg
    13801 gcgcaaggat cagggccaga aaacctgcag ccagcgtcac cccagtatcg tagtgaccaa
    13861 aatccagctc gccgagatag gaaaaaccaa agtagaccgc caccagagca attgacagcg
    13921 aggtaaaaaa ttcgagaatg ccggaggata aaaatgccag ccgtagcact tccattgtcc
    13981 gttggcggaa atcttccgaa gcagaacgaa tactttcaat ttcagcttca ccacgaccaa
    14041 aaatacgcaa tgtttccatg ccgcgcaggc gatcgaggaa atgcccactt aagcgagcaa
    14101 gagcgagaaa gttacgtcgg ttagcatcgg cagcccccat tccaaccagc gccataaaca
    14161 acggaattaa cggtgcagtg cccagcagaa tgagcgccgc agcccagttt gaggggaaga
    14221 ttgccaccac aatcagcaac ggcaccgaca ctgccagcgc catttgcggc aggtagcgtg
    14281 catagtaatc atgcatatcg tcaatttgct cgagtaccag cgtcgcccag ctccccgcag
    14341 gtttaccctg aatccacgct ggccctgctt gttgcagacg gtcgagaacc tgccggcgga
    14401 tggcaaagcg gatatgctgc ccggcgtgat aacccacccg ttcgcgtaac cagaccaccc
    14461 atgcgcgcag tacaaaggtc agaaccagta acgtaaaggg aagcagcagg gcttcacggg
    14521 gaatattctc cataatcata tgttgcagaa ttcgcgccat gaaccaggcc tgggcaatga
    14581 tcaatatgcc gctcacaaag cccagcagac gagaaatatt cagccaacgt tgggagatga
    14641 cgctttgctg ttttaaccag cgggttaact ctttttgacg agatttattc attgcacgct
    14701 tagcaggtga gttatcagaa ttatttgcag agcaatgtta caacggggaa aaaataaagg
    14761 cgacccatag tcgcatggtg tcgccttctt tacttttgtt actgatttgt aaaattattt
    14821 tgcgtcagct aaaccatcga ggtagcgttc cgcatcaagt gctgccatgc agcctgtacc
    14881 agcagatgta atggcctggc gataaatgtg atccatcacg tcgcctgcgg caaagacgcc
    14941 aggaatgctg gtctgggtgg cattaccatg aatacccgac tgcactttga tgtagccgtt
    15001 ttccagttcc agctgccctt cgaaaatggc agtattcggg ctgtgaccga tagcaacaaa
    15061 cagaccggca acgtcgagtg actcgatgtt atcgctgttt tgcgtatcgc gcagacgaac
    15121 gccagtgaca cccatttgat cgccggtcac ttcttccagc gtacggttgg tgtgcagaat
    15181 gatgttgccg ttctccactt tatccatcag acgcttaata aggatttttt ccgcgcggaa
    15241 accgtcacgg cggtgaatca gatgcacttc cgaagcgatg ttagacagat acagcgcctc
    15301 ttcaaccgcg gtattgccgc cgccgatgac cgcaactttc tggttgcgat agaagaaacc
    15361 gtcgcaggtt gcacaagcag aaaccccacg gcctttaaag gcttcttcag agggcaggcc
    15421 gagatagcgt gcagaagctc cggtggcaat aatcagcgcg tcgcaagtgt attcgccgtt
    15481 atcgccattc agacggaacg gacggttttg cagatccacc ttgttgatat gatcaaaaat
    15541 gatctcagtt tcaaacttgg tggcatggtc gtgcatgcgc tccattaata acggaccggt
    15601 cagatcgttt ggatcgccag gccagttttc cacttccgtg gtggtggtca gttggccgcc
    15661 tttttccatg ccggtaatca gcacaggttg caggttggcg cgcgccgcgt agacagcagc
    15721 ggtgtatccc gccgggcctg aacccaggat aagcagttta ctgtgtttgg tcgtgcccat
    15781 gagatcccca tagttgttgg cagacaatgg gcaggattgt agggaattta cagacgtaaa
    15841 aaaagagtat gacgattttg ttaacaattt gtgcaatcgg cagcatcgat aagcaggtca
    15901 aattctcccg tcattatcac ctctgctact taaatttccc gctttataag ccgattaaat
    15961 gatgaataaa cgcccctgtt aatgaatatc tggcatgttg tactaaaaat cgatgttttg
    16021 ctttgacaat cccctggtgt tttgcgaaaa cattcgagga agaaaaaaaa cagtattctt
    16081 atatgcgcat aaccatgcat gtaaatacca tgtttaccgt gctagtgaaa tctacgtatg
    16141 gcgtggacag acgccattcg tgatgtcgat agctgccaca aggcaacggt cttctcaccg
    16201 tagacccagg cattgcgcgc cgtgaatctt catgatttcg gtctatcgtg acgggtagcg
    16261 actctgaaca gtgatgtttc agggtcagac aggagtaggg aaggaataca gagagacaat
    16321 aataatggta gatagcaaga agcgccctgg caaagatctc gaccgtatcg atcgtaacat
    16381 tcttaatgag ttgcaaaagg atgggcgtat ttctaacgtc gagctttcta aacgtgtggg
    16441 actttcccca acgccgtgcc ttgagcgtgt gcgtcggctg gaaagacaag ggtttattca
    16501 gggctatacg gcgctgctga acccccatta tctggatgca tcacttctgg tatttgttga
    16561 gattactctg aatcgtggcg caccggatgt gtttgaacaa ttcaataccg ctgtacaaaa
    16621 acttgaagaa attcaggagt gtcatttagt atccggtgat ttcgactacc tgttgaaaac
    16681 acgcgtgccg gatatgtcag cctaccgtaa gttgctgggg gaaaccctgc tgcgtctgcc
    16741 tggcgtcaat gacacacgga catacgttgt tatggaagaa gtcaagcaga gtaatcgtct
    16801 ggttattaag acgcgctaac acggaacagg tgcaaaatcg gcgtattttg attacactcc
    16861 tgttaatcca tacagcaaca gtactggggt aacctggtac tgttgtccgt tttagcatcg
    16921 ggcaggaaaa gcctgtaacc tggagagcct ttcttgagcc aggaatacac tgaagacaaa
    16981 gaagtcacat tgacaaagtt aagtagcggc cgccgccttc tggaggcgtt gctgatcctt
    17041 attgtcctgt ttgccgtctg gttgatggct gccttactaa gctttaaccc ttcggacccc
    17101 agctggtcgc aaacggcctg gcatgaacct atccataatt taggtgggat gcccggtgcg
    17161 tggttggcag atacgctgtt ctttattttt ggcgtgatgg cttacaccat tcccgtcatt
    17221 attgtcggcg gttgttggtt tgcctggcgt catcagtcca gcgacgaata cattgattat
    17281 tttgccgttt cgctacgcat cattggcgtt ttggcgctca tccttacctc ctgtggtctg
    17341 gcggcaatca acgctgacga tatctggtat tttgcctccg gtggcgtcat tggcagctta
    17401 ctaagcacta cgctacaacc actgctacac agtagcgggg gaactattgc gctgctctgc
    17461 gtttgggcag cgggcctgac gttgttcacc ggttggtcat gggtgaccat tgctgaaaaa
    17521 ctcggcggct ggattttaaa cattctcacc ttcgccagta atcgtacccg tcgcgatgat
    17581 acctgggtcg atgaagatga gtatgaagac gacgaagagt atgaagatga aaatcacggc
    17641 aaacagcatg aatcacgccg tgcccgtatt cttcgcggcg cgctagcgcg tcgtaaacgg
    17701 ttggcggaaa aattcattaa tccgatgggg cggcaaacag acgctgcgtt gttctccggt
    17761 aagcggatgg atgatgacga agagattacc tacactgcac gcggtgtggc tgccgacccg
    17821 gacgacgtcc tattttcggg caatcgtgca acgcagccag aatatgacga atacgatcca
    17881 ttattaaacg gtgcgccaat taccgaacct gtcgctgtag cagctgctgc taccacggcg
    17941 acacaaagct gggctgcgcc ggttgaacct gtgactcaga cgccgcctgt tgcctctgtt
    18001 gatgttccac ctgcgcaacc tacagtagcc tggcagcctg taccgggtcc acaaacggga
    18061 gagccggtta ttgctcctgc accggaaggt tacccacagc agtcacaata tgcgcagcct
    18121 gcagtgcaat ataatgagcc gctgcaacaa ccagtacagc cgcagcagcc gtattatgca
    18181 cctgcagctg aacaacctgc gcaacagccg tattatgccc ctgcgccaga acaaccggtg
    18241 gcaggtaacg cctggcaagc cgaagagcag caatccactt ttgctccaca gtctacatac
    18301 cagactgagc aaacttatca gcagccagcc gctcaggagc cgttgtacca acagtcgcaa
    18361 cccgttgaac agcagcctgt tgtggagcct gaacccgttg tagaagagac aaaacccgcg
    18421 cgtccgccgc tttactactt tgaagaagtt gaagagaagc gagcccgtga acgtgaacaa
    18481 cttgcggcct ggtatcaacc gattccagaa ccggttaaag aaccagaacc gatcaaatct
    18541 tcgctgaaag caccttctgt tgcagcagta cctccagtag aagccgctgc cgctgtttcc
    18601 ccgctggcat ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc aaccgttgcc
    18661 gcgccagtct tcagtctggc aaatagcggt ggaccgcgtc ctcaggtcaa agaggggatt
    18721 ggtccgcagt tgccacgacc gaaacgtatc cgcgtgccaa ctcgtcgtga actggcgtct
    18781 tacggtatta agctgccctc acagcgtgcg gcggaagaaa aagcccgtga agcccagcgc
    18841 aatcagtacg attctggcga tcagtacaac gatgatgaaa tcgatgcgat gcagcaggat
    18901 gaactggcac gtcagttcgc ccagacacag cagcaacgct atggcgaaca gtatcaacat
    18961 gatgtgcccg taaacgcaga agatgcagat gctgcggcag aggctgaact ggctcgtcag
    19021 tttgcgcaaa ctcaacaaca acgttattcc ggcgaacaac cggctggggc gaatccgttc
    19081 tcgctggatg attttgaatt ttcgccaatg aaagcgttgc tggatgatgg tccacacgaa
    19141 ccgttgttta cgccaattgt tgaacctgta cagcagccgc aacaaccggt tgcaccgcag
    19201 cagcaatatc agcagccgca acaaccagtt ccgccgcagc cgcagtatca gcagccacaa
    19261 cagccggttg cgccgcagcc acaatatcag cagccgcaac aaccggttgc gccacagcag
    19321 caatatcagc agccgcaaca accggttgcg ccgcagcagc agtatcagca gccacaacag
    19381 ccagttgcgc cacaaccgca ggataccctg cttcatccgc tgttgatgcg taatggcgac
    19441 agccgtccgt tgcataaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg
    19501 ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggctcg cctggtggaa
    19561 gcgcgtctgg ctgatttccg tattaaagcc gatgtcgtca attactctcc ggggccggtt
    19621 atcactcgct ttgaattgaa cctggcaccg ggcgtaaaag cggcgcgcat ttctaacttg
    19681 tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct
    19741 ggcaaaccct atgtaggtct ggagttaccg aataaaaaac gacaaaccgt ttatctgcgc
    19801 gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt ggtgctgggt
    19861 aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttggtt
    19921 gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt
    19981 tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt
    20041 tcggtttatg aaggcattcc gcatctgtta acggaagtcg ttactgatat gaaagatgcc
    20101 gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc ggtataaact gatgtctgcg
    20161 ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg
    20221 cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg
    20281 ctgaaaaaag aaccatacat tgtggtgttg gttgacgaat ttgccgacct gatgatgacg
    20341 gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt
    20401 atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa
    20461 gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt
    20521 cttgatcagg ctggcgcgga atcactgctg ggtatggggg atatgctcta ctctgggccg
    20581 aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc
    20641 gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacctccgac
    20701 agcgaaagcg aaggtggtgc gggtggtttc gatggcgctg aagaactgga tccgttgttc
    20761 gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt
    20821 cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg
    20881 attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc gccgtttgac
    20941 taattaatgc attgccggat aaggtgcggt agcgtcgcat ccggcactct atcaactgaa
    21001 aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga
    21061 actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg ccatcacctg
    21121 tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa
    21181 aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg
    21241 tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt
    21301 caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg
    21361 gttctataac ccgttcgttg agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa
    21421 tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa
    21481 acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt
    21541 caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga
    21601 tcagcgcagc agttatcaac tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt
    21661 taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag
    21721 tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc
    21781 ggccagaaaa tttagcacag tatatcggcc agcaacattt gctggctgcg gggaagccgt
    21841 tgccgcgcgc tatcgaagcc gggcatttac attctatgat cctctggggg ccgccgggta
    21901 ccggcaaaac aactctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta
    21961 tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa
    22021 accgcaatgc aggtcgccgc actattcttt ttgttgacga agttcaccgt ttcaacaaaa
    22081 gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa
    22141 ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtctatc
    22201 tgttgaaatc cctgagtaca gaggatattg agcaagtact aactcaggcg atggaagaca
    22261 aaacccgtgg ctatggtggt caggatattg ttctgccaga tgaaacacga cgcgccattg
    22321 ctgaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata
    22381 tggccgaagt cgatgatagc ggtaagcggg tcctgaagcc tgaattactg accgaaatcg
    22441 ccggtgaacg tagcgcccgc tttgataaca aaggcgatcg cttttacgat ctgatttccg
    22501 cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctgtactgg tatgcgcgaa
    22561 ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ctgtctggcg attgcgtctg
    22621 aagacgtcgg taatgccgat ccacgggcga tgcaggtggc aattgcggcc tgggattgct
    22681 ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg
    22741 cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agccgcgctg gccgatgctc
    22801 gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga
    22861 aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg
    22921 ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc ccgacaaaca
    22981 ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata
    23041 gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt
    23101 ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg
    23161 atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct
    23221 ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca
    23281 aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag
    23341 cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg
    23401 atgcagcaaa agccgagctg gatgctttac aggctgaaat tcgcgatatc gcgctgacca
    23461 tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag
    23521 tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg
    23581 gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg
    23641 tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc
    23701 tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca
    23761 cgctgtacgg tacgggtcaa ctgccgaaat ttgctggcga tctgttccat actcgtccgc
    23821 tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa gttccgctga
    23881 ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc
    23941 acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc
    24001 gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccagaa gactcaatgg
    24061 cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt
    24121 accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa acttacgacc
    24181 tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc tccaacgttt
    24241 gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac aagaaaaccc
    24301 gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa
    24361 tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg cgtccgtata
    24421 tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa agcgcctgcg
    24481 ggcgcttttt ttgtctccct ttgataccga acaataatta ctcctcactt acacgtaata
    24541 ctactttcga gtgaaaatct acctatctct ttgattttca aattattcga tgtatacaag
    24601 cctatatagc gaactgctat agaaataatt acacaatacg gtttgttact ggaatcaatc
    24661 gtgagcaagc ttgagtgagc cattatgaaa acgaaaatcc ctgatgcggt attggctgct
    24721 gaggtgagtc gccgtggttt ggtaaaaacg acagcgatcg gcggcctggc aatggccagc
    24781 agcgcattaa cattaccttt tagtcggatt gcgcacgctg tcgatagcgc cattccaaca
    24841 aaatcagacg aaaaggttat ctggagcgcc tgtacagtta actgtggtag tcgctgcccg
    24901 ctacgtatgc acgtcgtgga cggtgaaatc aaatatgtcg aaacggacaa taccggcgat
    24961 gacaattacg acggcctgca ccaggttcgc gcctgcctgc gtgggcgttc catgcgtcgc
    25021 cgtgtctaca atccggaccg cctgaaatat ccgatgaaac gagtcggggc gcgcggtgaa
    25081 ggcaaattcg agcgcattag ctgggaagaa gcctacgaca tcatcgcgac caatatgcag
    25141 cgcctgatca aagagtacgg caacgagtct atctatctga actatggcac cggtacgctg
    25201 ggcggcacca tgacccgctc ctggccgccg ggaaataccc tggtcgcgcg gctgatgaac
    25261 tgctgcggcg gctatctgaa ccattacggc gactactcct ccgcgcaaat tgcggaaggt
    25321 ttgaactata cctacggcgg ctgggcagat ggcaacagcc cgtcggatat cgaaaacagt
    25381 aagctggtag tgctgtttgg taataaccct ggcgaaacgc gaatgagtgg cggtggggtg
    25441 acttactatc ttgaacaggc acgccagaaa tctaatgccc gcatgatcat catcgatccg
    25501 cgctataccg acaccggtgc cgggcgcgaa gatgagtgga tccctattcg tccgggaaca
    25561 gatgccgcac tggttaacgg tctggcgtac gtcatgatca ctgaaaacct ggtggatcag
    25621 gcattcctcg ataaatattg cgttggctac gatgagaaaa ccctgccagc cagtgcgccg
    25681 aaaaatggcc actataaagc ttatattctg ggtgaagggc cagatggcgt ggctaaaacg
    25741 ccggaatggg cctcgcaaat cactggtgtt ccggcagaca aaatcatcaa attggctcgt
    25801 gaaatcggta gtaccaaacc ggcgtttatc agccagggat ggggcccgca gcgtcacgct
    25861 aacggtgaaa tcgcaacccg tgctatctcg atgctggcga ttctgaccgg taacgttggt
    25921 attaacggag gcaacagcgg cgcgcgtgaa ggttcataca gcttaccgtt tgtccgtatg
    25981 ccgaccttgg aaaacccgat ccagaccagc atttcgatgt ttatgtggac cgatgccatt
    26041 gaacgtggcc cggaaatgac ggcgctgcgt gatggtgtac gcgggaaaga taagctggat
    26101 gtgccgatca aaatgatctg gaactatgcc ggtaactgcc tgattaacca gcattctgaa
    26161 atcaaccgta cccatgaaat ccttcaggat gataagaagt gcgagctgat tgtggttatc
    26221 gactgccaca tgacctcatc ggcgaaatat gctgacatcc tgctgcctga ctgcaccgct
    26281 tccgaacaga tggactttgc gctggatgca tcctgcggga atatgtctta cgtgattttc
    26341 aacgatcagg tgattaaacc gcgctttgaa tgtaagacca tctatgaaat gaccagcgaa
    26401 ctggcaaaac gtcttggcgt tgagcaacag tttactgaag gccgtaccca ggaagagtgg
    26461 atgcggcatc tgtatgccca gtcgcgggaa gcgattcctg aactgccaac gtttgaagag
    26521 ttccgcaagc aggggatctt taaaaagcgc gacccacaag ggcatcacgt tgcttataaa
    26581 gccttccgtg aagatccgca ggcaaaccca ctgactacgc catcgggcaa aattgagatt
    26641 tattcgcagg cgctggctga cattgccgct acctgggaat tgcctgaagg cgatgtgatc
    26701 gatccactgc cgatctacac gccgggcttt gaaagttatc aggatccgct gaacaaacag
    26761 tatccgctgc agcttacagg tttccactat aaatctcgcg ttcactcaac ttacggcaac
    26821 gttgatgtgc tgaaagcggc ttgccgtcag gaaatgtgga tcaacccgct tgatgcccaa
    26881 aaacgcggta tccacaacgg cgataaagtc aggatcttta acgatcgtgg tgaggttcat
    26941 attgaggcga aagtgacgcc acgaatgatg ccgggtgtgg tcgcactggg tgaaggtgcc
    27001 tggtatgacc cggatgcaaa acgtgtcgat aagggtggtt gtattaacgt actgaccact
    27061 caacgtccgt ctcctctcgc taaggggaat ccgtcacata caaaccttgt tcaggttgaa
    27121 aaggtgtaag gagtaaccga tgacaaccca gtatggattt tttattgatt ccagccgttg
    27181 caccggttgc aaaacctgcg agctggcctg taaagactac aaagatttga cgccagaagt
    27241 cagcttccgc cgcatttatg aatatgctgg cggcgactgg caggaagata acggtgtctg
    27301 gcaccagaac gtgtttgcct actatctgtc gatttcatgt aaccactgcg aagatccggc
    27361 ttgtactaaa gtctgcccga gcggtgcgat gcataaacgt gaagatggtt ttgttgtggt
    27421 cgatgaagat gtgtgcattg gctgccgcta ctgccatatg gcttgcccgt atggcgcacc
    27481 gcaatataac gaaacgaaag gccatatgac caaatgcgat ggttgttatg accgtgttgc
    27541 ggagggtaaa aagccgatct gtgttgaatc ttgtccgctg cgggcgctgg atttcggccc
    27601 tatcgacgag ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt tgccgcgagc
    27661 tcactttacc aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc cgaccgggga
    27721 taccactggc tatctggcaa acccgaagga ggtgtgagat gggaagtgga tggcatgaat
    27781 ggccgctgat gatcttcacg gtcttcgggc aatgtgtagc aggtggtttt atcgttctgg
    27841 ctttggcgct gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt atcgcctgca
    27901 tgtttggttt atgggtgctg atgggcattg gctttatcgc ctctatgctc catcttggtt
    27961 caccaatgcg cgcttttaac tcgctcaacc gggtaggggc ttcggcactc agtaacgaaa
    28021 tcgccagcgg ttcgatcttt tttgccgtag gcggcatcgg ctggttgctg gcaatgctga
    28081 aaaagctgtc accggcattg cgtacgctgt ggctgatagt gacgatggtt cttggcgtca
    28141 tctttgtctg gatgatggtg cgtgtgtata acagcattga taccgttccg acctggtaca
    28201 gcatctggac gccgatgggc ttcttcctga cgatgtttat gggcggcccg ttactgggtt
    28261 acctgttgtt gagtctggct ggcgtcgatg gctgggcgat gcgtctgctg ccagcaattt
    28321 ctgtactggc actggtagtg agtggcgtgg tgtcagtgat gcagggcgca gagctggcga
    28381 ctattcatag ttctgtgcag caggccgcag tgctggtgcc ggactatggt gcgctgatgt
    28441 cctggcggat cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca cagctaaaag
    28501 gttatcagcc tgcggttccg ctactttctg tatcattcat tctgcttctg gcaggggaat
    28561 taatcggtcg cggcgtattc tatggtttgc atatgaccgt ggggatggcc gtcgcaagct
    28621 aatcataaca accggggttt cggccccggt tctcttttat ttctgcttcg ttaacgtgtc
    28681 ataactggtc atcaaattac gataatcagg aatgtggtta gagaacaacg tcgccagtcc
    28741 ttcaatatca ttacgccagt cgcgatgcag ctcgcacgcc acgccgaacc atgtcatcaa
    28801 ttgcgcacca gcttgcgaca agcgatccca tgccgaatgc cgggtaattt cattaaaggt
    28861 ccctgacgcg tcagtcacga caaagacgtc aaacccttct tcaatggctg aaagcgccgg
    28921 gaatgccacg caaacttcgg ttaccacacc ggcaataatt aactgttttt tacctgtcgc
    28981 tttgacagct tttacaaaat cttcgttatc ccaggcgtta atatttcccg ggcgagcaat
    29041 gtaaggtgta tcggggaatt gtgctttcag ttctggaact aatgggccgt taggaccggt
    29101 ttcaaaactg gtggtaagaa tagttggtaa gttgaaatac ttagccaggt cacccagcgc
    29161 cagcacgtta tttttaaact tatcgggttc gatatcccgt acaagggaaa gtaaaccagc
    29221 ctggtgatca acaagcaaaa cggcagcatc atttttatca agacgaacat acggtttggt
    29281 catcctcttc tcctttcgag agtcagtggt acgtctgaaa tcgatcagac gcgatgcatt
    29341 gctctgaaag catagacggg aaatatgagt ttgctgtgac catgaaattt ttcgactgaa
    29401 cgaagcgtat gaaatttgtg ttagttcaat aaaaacaatc agatgagact aatcatctct
    29461 tcagcattag cgttatgaat gagtaatata aatcgataat accgccgctg ggatatcgcg
    29521 tattttcacc cattgacaat gtttttggcg gtggcatgat gcgcatgaaa tttgaacttc
    29581 ctcacggttt taattcatgt ccacgtatac ccggcctgtc atgcttttgc tgtctggcct
    29641 gcttttgttg actctggcga ttgcggtgtt aaatacactc gtgccgcttt ggctcgccca
    29701 ggaacacatg tccacatggc aggtaggcgt tgtcagctca tcctatttta ccggcaacct
    29761 tgtcggtaca ttgctgacag ggtatgtcat taagcgcatt ggctttaacc gcagctatta
    29821 tctggcctcc ttcatttttg ccgctggctg tgccggcctt ggcctgatga ttggattctg
    29881 gagctggttg gcttggcgtt ttgtcgcggg cgtcggctgt gccatgattt gggtggttgt
    29941 tgagagcgcg ctgatgtgca gtgggacgtc acgtaaccgt gggcgtttgc ttgctgcgta
    30001 tatgatggtt tattacgtgg gaacgttttt aggccagtta ctggtcagca aagtttcaac
    30061 cgagctgatg tccgtattgc cgtgggttac aggtttgacg ttggcaggga tcttaccgct
    30121 gttgtttacg cgtgtgctga atcagcaggc tgaaaaccat gattcgacgt caattacgtc
    30181 aatgctaaaa ctccgtcagg cgcggcttgg cgtgaatggc tgcattatct caggaatcgt
    30241 tctgggatct ctatatggcc tgatgccgct gtacctcaat cacaaagggg tgagcaatgc
    30301 cagcattggt ttctggatgg cggtactggt cagtgcgggt atccttggac aatggccgat
    30361 tggacgtctg gcggataagt ttggtcgact gctggtgttg cgtgttcagg tctttgtcgt
    30421 cattctcggc agtatcgcga tgcttagcca ggcggcgatg gccccagcgt tattcatcct
    30481 cggtgccgct ggctttacgc tatatccggt ggcgatggca tgggcttgcg agaaagttga
    30541 acatcatcaa ctggtggcga tgaaccaggc cttactgttg agctatactg tgggaagtct
    30601 gcttggcccg tcatttaccg ctatgctaat gcagaatttc tccgataatt tattgtttat
    30661 catgatcgcc agcgtatcgt ttatctattt gctgatgctg ctgcgcaacg ccggtcatac
    30721 gccgaaaccc gttgctcacg tgtaaatgaa ttcaagcaga gtgtgaactt actgtttcac
    30781 actctgcttt tttgtttctt ctatctgact tgctttattc caaattttat tcgtttaaaa
    30841 ataaaatgtg cagcaggtta taattttgca tttcgctatt tccgcacttc ttatttgccg
    30901 cgcataatcc ctcgttttac agatgcccct ttaattttgg cgaaggattt gtctatggct
    30961 gggaatgttc aggaaaaaca gttgcgatgg tacaacattg cgctgatgtc ttttatcact
    31021 gtctggggtt ttggcaacgt tgttaataac tatgccaacc aggggctggt ggttgttttt
    31081 tcatgggtgt ttatctttgc actctatttc acaccttatg cgctaattgt tggtcagtta
    31141 ggctcgacct tcaaagatgg gaagggcggg gtcagtacct ggattaaaca cacgatggga
    31201 cccggactgg cttatctcgc cgcgtggacc tactgggtgg tgcatattcc ctatctggca
    31261 caaaaacccc aggcaattct gattgcgctc ggttgggcga tgaaaggcga cggttcgcta
    31321 atcaaagaat attcagtcgt agcgttacag gggttaacgc tggtgctgtt tatcttcttt
    31381 atgtgggttg cttcacgcgg tatgaaatcg ctgaaaatcg tcggttctgt ggcagggatt
    31441 gctatgtttg ttatgtcgct cctgtatgtg gcgatggcgg taaccgcgcc tgcaattact
    31501 gaagtgcata ttgcgaccac aaacattacc tgggaaacgt tcattcctca tatcgacttt
    31561 acctacatta ccactatttc aatgctggtt ttcgcggttg gcggagcaga gaagatttct
    31621 ccttacgtta atcaaacgcg caacccagga aaagaatttc caaaagggat gttatgcctg
    31681 gcggtgatgg ttgcggtttg tgccattctg ggctcgctgg cgatggggat gatgtttgat
    31741 tcgcgtaata tcccggatga cttaatgacc aacggtcagt attacgcctt tcagaagctg
    31801 ggcgagtatt acaacatggg taatacttta atggtgattt acgccattgc gaataccctg
    31861 ggacaagtag cggcgctggt attctcgatt gatgccccgc ttaaagtgct attaggtgat
    31921 gctgacagca aatatattcc agccagttta tgtcgtacca acgcttctgg tacgcccgtt
    31981 aatggctatt ttctgaccct ggtactggtg gcgattctga ttatgctgcc gactctcggc
    32041 attggtgata tgaacaatct ctataaatgg ctgttgaacc ttaattcggt agtgatgccg
    32101 ctgcgttatt tatgggtatt tgttgcattt attgcagtcg ttcgcttggc gcagaaatat
    32161 aaaccagaat atgtctttat tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt
    32221 tttgccttta ccgcctttgc ctgtttgacg gggatcttcc cgaaaatgga agccttcact
    32281 gcagagtgga ccttccagtt ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta
    32341 ggactgatat tcccgctgct ggcgcgtaaa gcgaatagta aataattatt gtggtcggtg
    32401 acgctgactc tggcagatct gacccgtttt tacataatcg agaaatgcgc gcaagccgca
    32461 ggacatatac tggcggtttg gataatagat ctggaagcct ggacgctctg tgctccagtc
    32521 ttccagtaca cattctaaac gaccagtatc aaggtactcc ttgatctgtt catagagcag
    32581 atacccgata ccagcaccca tcaaaacggc ctccagttca gcatcaacat catccagaat
    32641 gatatttcct gcgacagcaa tttccagctc ctttgcaaat tgccagtgga aaggtttgcc
    32701 gctgggataa cgaaaaacaa cacattgatg attaagcaga tcatgaggat ggcggggttt
    32761 gccatatctt gcaaagtact ctggcgttgc tgctacacat aatttcactg gcgggccgat
    32821 cgccacgctg atcatatctt tttcgacgat gcaactaagt cgaacgccag cgtcaaagcc
    32881 ctgttggacg atatcgacaa gagagtcgtc agttgtgagc tcgactttaa tatcaggata
    32941 ttcacgtgtg aaaccaacca gtaaaggcat taaaaatata cgtgcagcta cccgggcggc
    33001 attgattttg agcgttcctg tcggtgtcag acgaaaatca ttcatctcat ctaacatgat
    33061 ctgaatttcg tcgaaagctg ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa
    33121 cgatacgctt cgggttgtcc gattgaataa gcgaatttta agacgttgtt ccagtgtttt
    33181 aatactatgg ctaatggcgg acgaggataa gcctaactca tcgcccgctg cacgaaagct
    33241 ttgattacgg gccacggcaa agaaagtggc aaagtcagac atattcatcc gcattagtga
    33301 aaatccttca ttaactcatc ctgaatcatc acgtttacag gattatgccg cctgcgtaaa
    33361 gtgcgctcca gaacttaacg tggaggtaaa attatgcagt ctgaacgtat ttatttggta
    33421 tgggcccatc ctcgtcatga ttcattgacc gcacatattg ctgatgcgat ccatcagcgg
    33481 gcaatggagc ggaaaataca ggtgacggaa ctcgatttat atcggcgtaa tttcaaccca
    33541 gtgatgacgc cggaagatga accagactgg aagaatatgg ataaacgtta ttctccagag
    33601 gttcatcagc tttattcaga gctgcttgaa catgacacgt tagtggtggt ttttcctctc
    33661 tggtggtaca gcttcccggc aatgctaaaa ggatatattg acagagtatg gaataatggg
    33721 ctggcttatg gagatgggca caaattacca ttcaataaag ttcgttgggt ggcgctggtt
    33781 ggaggagaca aagaatcatt tgtccagatg ggctgggaaa aaaatataag cgattattta
    33841 aaaaatatgt gcagttatct tggtattgaa gatgccgatg tcactttctt gtgtaataca
    33901 gtggtattcg atggggaaga acttcacgcg agctattatc agtcgttatt atctcaggta
    33961 cgggatatgg tagatgcact ataagatgtg ttaaaaacgc tgtagcagaa tgaagcgcgg
    34021 aataaaaaag cggcaactca ataaagttgc cgctttacgg ggaaattaga acattacctt
    34081 atgaccgtac tgctcaagaa tgcctttcac gcgttccatg gtctctttct tcggtggttt
    34141 aacaccgtcg agtttgtact cttcacccat tgccacccat ttgtgtttgc ccagctcgtg
    34201 gtaggggaga agctcgattt tctcaacgtt gcccatatca cgggtaaatt caccgaggcg
    34261 atgcgctgaa tcgtcatcgt cagaccagcc tgggacaaca acgtagcgga tccacacctt
    34321 cacatttttg ttcgccagat atttagcgaa ctccagcgtg cggtggttgg aaactccaac
    34381 cagattttgg tggatctcgt cgttcatctg tttgagatcg agcattacca ggtcggttac
    34441 ttccagcagt tcatcaatca ccggatcgta acgacgaaca aaaccgttgg tgtccagaca
    34501 ggtatgaatg ccttcttttt tgcaggcgcg gaaccagtca cgaacaaact cagcttgcag
    34561 gattgcttca ccgccggatg cggtaacgcc gccgccggaa gcgttcataa agtggcgata
    34621 ggtcaccact tccttcatca aatcttcaac ggtaacttct ttaccgccat gcgtgtccca
    34681 ggtgtcgcgg ttatgacaat acaggcagcg catcaggcag ccctggaaaa aggtgataaa
    34741 gcgaatacct gggccgtcta cggttccaca ggattcaaag gagtgaatgc gaccaataac
    34801 tgacattgcg gtgtttctcc agatgtggcc catctgaggc cgtgttggtg cgcagctcga
    34861 aggctacgtc gagtctgttt tggcagtcac cttaaagtat agatagctga caaaaaaggc
    34921 tctcgcgcta aaaaaggccc cactttcgtg gagcctttat tgtacgcttt ttactgtacg
    34981 atttcagtca aatctaatta catagattga gtgaaggtac gagtaataac gtcctgctgc
    35041 tgttctttag tcagcgagtt gaaacgtact gcgtagccag atacacggat ggtcagctgc
    35101 ggatattttt ccgggttttc catcgcgtcg agcagcattt cacggttcat cacgttaacg
    35161 ttcaagtgct gaccaccttc gatggatgct tcgtggtgga agtaaccatc catcagacca
    35221 gccaggttgg tcttacgaac ttcgtcgtct ttacccagtg cgttcggaac gatagagaag
    35281 gtgtaggaga taccatcttt agcgtaagca aacggcagtt tagcaacgga agtcagagag
    35341 gctactgcac ctttctggtc acgaccgtgc atcgggttag cacccggtcc gaacggcgcg
    35401 ccagcacgac gaccgtctgg ggtgttaccc gttttcttac catacacaac gttagaagtg
    35461 atggtcagaa cagactgagt cgggatagcg tcacggtagg tgtgcagttt ctgaattttc
    35521 ttcatgaaac gttctaccag gtcaacagcc aggtcatcta cacgcggatc attgttacca
    35581 aactgcgggt attcgccttc gatttcgaag tcgatagcca gaccgtcttc gtcacgaatc
    35641 ggtttaactt tcgcatattt gattgcagac agggagtcag cagcaacgga cagaccagcg
    35701 ataccacacg ccatggtgcg gataacgtca cggtcgtgca gcgccatcag agaggcttcg
    35761 tagctgtact tgtcgtgcat gtagtggatg atgttcagtg cagtgatgta ctgtttagcc
    35821 agccagtcca tgaagtgatc catgcgctcc atcacttcat catagttcag gacatcgcct
    35881 ttgatcggtt cagacttcgg accaacctgc attttcagtt tttcgtcaac gccgccgttg
    35941 attgcgtaca gcatggtttt cgccaggttt gcacgcgcac cgaagaactg catttgttta
    36001 ccaacgatca tcgggcttac gcagcaagca atagcgtagt catcgttgtt gaagtccgga
    36061 cgcatcaggt catcgttctc atactgcaga gaagaggtgt cgatggacac tttagcggcg
    36121 aatttcttga agttcagcgg cagtttttca gaccacagaa tggtcatgtt cggttccgga
    36181 gacggaccca tggtgtacag ggtgttcagg aaacggaagc tgtttttggt aaccagggta
    36241 cgaccgtcga ggcccatacc accgatagat tcggttgccc agatcgggtc gccagagaac
    36301 agttcatcgt attccggagt acgcaggaag cgaaccatac gcagtttcat gaccaggtgg
    36361 tcaaccattt cctgcgcttc ttgttcggtg atcttgccag ctttcaggtc acgttcgatg
    36421 tacacatcca ggaaggtgga ggtacgaccg aaggacattg cagcaccgtt ctgagactta
    36481 acagcagcca ggtagccgaa gtaagtccac tggatagctt cctgagcgtt ggtagccgga
    36541 ccagagatgt cgtagccgta tttcgcagcc atttctttca tctgacccag agcgcggtgc
    36601 tgttcagcga tttcttcgcg cagacggata gtctgttcca ggtttacgcc gttttccaga
    36661 tcagcctgca gagaagtgaa ctgtgccagt ttgtctttca tcaggtagtc gataccgtac
    36721 agcgcaacgc gacggtagtc accgatgata cggccacggc catatgcatc tggcagaccg
    36781 gtcagaacac cagatttacg gcaacgcagg atgtccggag tgtaaacgtc gaacacgccc
    36841 tggttgtgag ttttacggta ttcagtgaag atttttttga tcatcggatc cagttcgcgg
    36901 ttgtacgctt tgcaggaacc ttcgatcatt ttgataccac cgaacgggat aagagcacgt
    36961 ttcagcggag cttcagtctg cagaccaacg attttctcaa gctgcttgtt gatgtagcca
    37021 gcgtcgtgag aggtgatggt ggaagcaaca gcggtgtcaa agtcaactgg cgcgtgagtg
    37081 cggttttcca gtttaacgcc ttccattact ttgtcccaca gggtggtggt cgcttcagta
    37141 gcgccagcca ggaaggactc gtcaccctcg tacggagtgt agtttttctg aatgaagtca
    37201 cggacgttta cttcattctg ccagtcacct ttggtaaaac cttcccaggc tgtggctaac
    37261 ttttcattaa gctcggacat gtaacaccta ccttcttaag tggatttttt atttactgcg
    37321 tacttcgaca accattaatg gtggtcgttt tcacgcaggt aaatgaccca gtatgtcaac
    37381 ccaaccaaca aaccaccacc gataatgttg ccgatcgtaa ccggaatcag gttatcagtg
    37441 atgaaattca tcacggtcag gtgagaaaaa ttttccggtg cagaaccgac tgcggtccaa
    37501 aattccgggg atgcgaagtc gcggattaca atacccatcg ggatcataaa catgtttgcg
    37561 atactgtgct caaaaccgct ggcaacaaac atcgcgaccg gcagcaccat aatgaacgct
    37621 ttgtccatca ggctgcggcc agaataactc atccatactg ccagacatac catcaggttt
    37681 gccaggatac caagacagac ggcctcaata aaagtatggt gcactttgtg gtcggcggtt
    37741 tgtaggacgt ttagtcccca ttgaccattt gcggtcatat actcgccgga aagccacatt
    37801 aaaagtacaa acagcagtgc gccgaccagg ttgccaaaat agacatttag ccagtttttc
    37861 gccaactgac cccaggtgat gcgcccactc gccttagcaa caacaatcaa cacggtggaa
    37921 gtaaagagat cggctccgca gacaacacaa agaatcagcc ccagagagaa gcaaatgccg
    37981 ccaaccagtt ttgccatgcc gaagggcatt gtgcctgtgc cagtggttgc tgtgatatag
    38041 aagacgaatg cgattgagat gaaaacaccg gcggtaatcg ccagatagaa agtcttaagc
    38101 ggatgtttcg ttgctttata gacacccgcc tcttcggcca ctttggccat tgcagcagga
    38161 agtaaaagat caaaagggtt gtcagctttc acactaactc tctctttatt aagtcggcga
    38221 cgagatacta acaaagcatt atagatgaga aattgatata gatcatatct cgcctggctt
    38281 ataggcccgt aactcgcatg gtttttatgc aaatacggag taaatatttg attatccaaa
    38341 taaaaataaa ttttaaaaat taacaaatga gttgaatttt ttccgcatcc tccgctaaaa
    38401 cagttaatta aa
//
LOCUS       DAJOSY010000030        38380 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJOSY010000030 DAJOSY010000000
VERSION     DAJOSY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.030"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            122..6772
                     /locus_tag="OHN27_001682"
     CDS             122..6772
                     /locus_tag="OHN27_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HCQ2286137.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEVETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSPLVEQNLMPWLATGAAALITALAVVELNDDDDHHHRNNSPL
                     PPTPPDDESDDTPVPPSPGGDEIIPDDPDDTPTPPKPISFNNDVILDKAEKTLTIRDS
                     VFTYTENADGTISLQDSNGRKATINLWQIDEANNTVALEGVSADGATKWQYNHKGELV
                     ITGDNATVNNNGKTTVDGKDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDSATVD
                     NKGTMTVTDPESIGIQIDGDKAVVNNEGESTITNGGTGTQINGDDATANNSGKTTVDG
                     KDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESIGIQID
                     GDKAIVNNEGDSSITNGGTGTQINGDEATTNNNGKTTVDGKDSTGTEINGNNGNVIQD
                     GDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESMGIQIDGDKAIVNNEGESTITNGG
                     TGTQINGDDATANNNGKTTVDGKDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDS
                     ATVDNKGTMTVTDPESIGIQVDGDQAVVNNEGESAITNGGTGTQINGDDATANNNGKT
                     TVDGKDSTGTEIAGNNGKVIQDGDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESIG
                     IQIDGDQAIVNNEGESTITNGGTGTQINGNDATANNSGKTTVDGKDSTGTKIAGNIGI
                     VNLDGSLTVTGGAHGVENIGDNGTVNNKGDIVVSDTGSIGVLINGEGATVSNTGDVNV
                     SNEATGFSITTNSGKVSLAGSMQVGDFSTGVDLNGNNNSVTLAAKDLKVVGQKATGIN
                     VSGDANTVNITGNVLVDKDKTADNAAEYFFDPSVGINVYGSDNNVTLDGKLTVVSDSE
                     VTSRQSNLFDGSAEKTSGLVVIGDGNTVNMNGGLELIGEKNALADGSQVASLRTGYSY
                     TSVIVVSGESSVYLNGDTTISGEFPLGFAGVIRVQDKALLEIGSGATLTMQDIDSFEH
                     HGTRTPELTYADSGAKIVNKGTVEIQNLGFAFVTGENTTGINSGTISLLQNGKDPAPS
                     PIVLLATNGGSATNAGTITGKVTEQHSVFNKYSTGTSNSFIFNNDVSSITGLVAQSNS
                     TIINTDSGIIDLYGRGSVGMLAIADSTAENQGKITLDSMWVDANDTTAMRDIASNSAI
                     DFGTGVGVGTDSYSGAGKNATAINQLGGVITIYNAGAGMAAYGASNTVINQGTINLEK
                     NGNYDDSLAANTLVGMAVYEHGTAINDQTGVININVGTGQAFYNDGTGTIVNYGTICT
                     FGVCQSGNEYNNTDDFTSLIYTGGDTITRSGETVTLNKSAAVTDKLAGNVVNSGTLSG
                     DQITVSSGLLENTSGGIINNLVKLDKGAVIKNAGVMTNNVDVSGGILNNAGEMTAQIT
                     MNAGADSSLVNNTGTINKIVQNAGVFNNSGSVTGRMMSAGGVFNNQTDGAIMRGAALT
                     GTAVANNEGTWNLGSSSEGNNTGMLEVNNNSAFNNRGEFILDNDKNAVHINQSGTLYN
                     TGHMNISNSSHNGAVNMWGGNGRFINDGTIDVSAKSLVVSANNAGDQNAFFWNQDNGV
                     INFDHDSASAVKVTHSNFIAQNDGIMNISGTGAVAMEGDKNAQLVNNGTINLGTAGTT
                     DTGMIGMQLDANATADAVIENNGTINIFANDSFAFSVLGTVGHVVNNGTVVIADGVTG
                     SGLIKQGDSINVEGMNGNNGNSSEVHYGDYTLPDVPKPNTVSVTSGSDEAGGSMNNLN
                     GYVVGTNVNGSAGKLKVNNASMNGVEINTGFTAGTADTTVSFDNVVEGSNLTDADAIT
                     STSVVWTAKGSTDASGNVDVTMSKNAYTDVATDASVNDIAKALDAGYTNNELFTSLNV
                     GTTAELNSALKQVCGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRA
                     ELGNNTEYDMLALRKTIDLSESQTMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLK
                     HQMSFDNGMNWNNALRYDVHNLDSSRSIAFGNTNKTADTDVKQQYLEFRSEGAKTFEP
                     SEGLKVTPYAGVKLRHTLEGGYQERNAGDFNLNMNSGSETAVDSIVGLKLDYAGKDGW
                     SASATLEGGPNLSYAKSQRTASLAGAGSQHFNVDDGQKGGGINSLTSVGVKYSSKESS
                     LNLDAYNWKEDGISDKGVMLNFKKTF"
     gene            6980..7841
                     /gene="pdxI"
                     /locus_tag="OHN27_001683"
                     /pseudo
     CDS             6980..7841
                     /gene="pdxI"
                     /locus_tag="OHN27_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
     gene            7905..10211
                     /locus_tag="OHN27_001684"
     CDS             7905..10211
                     /locus_tag="OHN27_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCQ2286138.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANAVDIEQFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIIR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            10382..10987
                     /locus_tag="OHN27_001685"
     CDS             10382..10987
                     /locus_tag="OHN27_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCQ2286139.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LTFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAEI"
     gene            10987..11883
                     /locus_tag="OHN27_001686"
     CDS             10987..11883
                     /locus_tag="OHN27_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCQ2286140.1"
                     /translation="MLEKSLATLFALLILATLINRFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSMVISGPRWMTLTFFALISFLALKEYCTIISVHFPRWLYWGIPLNYLLIGFNC
                     FELFLLFIPLAGFLILATGQVLVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLMGGVITIMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            11899..13656
                     /locus_tag="OHN27_001687"
     CDS             11899..13656
                     /locus_tag="OHN27_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCQ2286141.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKSSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWIHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRGASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALAFEKMQ
                     SFISRLYANKSQKFDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSSGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQEEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            13646..14962
                     /locus_tag="OHN27_001688"
     CDS             13646..14962
                     /locus_tag="OHN27_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCQ2286142.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCLRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVAAAWLLCYGHCKTVNEAISYIRARRPQIVLTDEHKAMLRLWENR"
     gene            complement(15016..15621)
                     /gene="azoR"
                     /locus_tag="OHN27_001689"
     CDS             complement(15016..15621)
                     /gene="azoR"
                     /locus_tag="OHN27_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCQ2286143.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVSA"
     gene            15822..19724
                     /gene="hrpA"
                     /locus_tag="OHN27_001690"
     CDS             15822..19724
                     /gene="hrpA"
                     /locus_tag="OHN27_001690"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCQ2286144.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRQRFLRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDTVDELSQESHGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            19996..20796
                     /locus_tag="OHN27_001691"
     CDS             19996..20796
                     /locus_tag="OHN27_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCQ2286145.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            20993..22432
                     /gene="aldA"
                     /locus_tag="OHN27_001692"
     CDS             20993..22432
                     /gene="aldA"
                     /locus_tag="OHN27_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCQ2286146.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(22474..23475)
                     /gene="gap"
                     /locus_tag="OHN27_001693"
     CDS             complement(22474..23475)
                     /gene="gap"
                     /locus_tag="OHN27_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCQ2286147.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            23664..24194
                     /gene="cybB"
                     /locus_tag="OHN27_001694"
     CDS             23664..24194
                     /gene="cybB"
                     /locus_tag="OHN27_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCQ2286148.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            24439..24612
                     /locus_tag="OHN27_001695"
     CDS             24439..24612
                     /locus_tag="OHN27_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286149.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(24684..24833)
                     /gene="hokB"
                     /locus_tag="OHN27_001696"
     CDS             complement(24684..24833)
                     /gene="hokB"
                     /locus_tag="OHN27_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCQ2286150.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(24933..26302)
                     /locus_tag="OHN27_001697"
     CDS             complement(join(24933..25822,25822..26302))
                     /locus_tag="OHN27_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCQ2286151.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
     gene            26677..28317
                     /gene="trg"
                     /locus_tag="OHN27_001698"
     CDS             26677..28317
                     /gene="trg"
                     /locus_tag="OHN27_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCQ2286152.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLSRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGISEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(28355..29278)
                     /locus_tag="OHN27_001699"
     CDS             complement(28355..29278)
                     /locus_tag="OHN27_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2286153.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILASLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            29495..30838
                     /locus_tag="OHN27_001700"
     CDS             29495..30838
                     /locus_tag="OHN27_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCQ2286154.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     LLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            31063..32718
                     /gene="opgD"
                     /locus_tag="OHN27_001701"
                     /gene_synonym="mdoD"
     CDS             31063..32718
                     /gene="opgD"
                     /locus_tag="OHN27_001701"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCQ2286155.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWKPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            32858..33082
                     /locus_tag="OHN27_001702"
     CDS             32858..33082
                     /locus_tag="OHN27_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCQ2286156.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            33145..33681
                     /gene="rimL"
                     /locus_tag="OHN27_001703"
     CDS             33145..33681
                     /gene="rimL"
                     /locus_tag="OHN27_001703"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCQ2286157.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIGS"
     gene            complement(33676..34656)
                     /locus_tag="OHN27_001704"
     CDS             complement(33676..34656)
                     /locus_tag="OHN27_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCQ2286158.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            34780..35772
                     /gene="tehA"
                     /locus_tag="OHN27_001705"
     CDS             34780..35772
                     /gene="tehA"
                     /locus_tag="OHN27_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCQ2286159.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFIARLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            35769..36362
                     /gene="tehB"
                     /locus_tag="OHN27_001706"
     CDS             35769..36362
                     /gene="tehB"
                     /locus_tag="OHN27_001706"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCQ2286160.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVMEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFAGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDSADYPCTVGFPFAFKERELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            36665..37333
                     /locus_tag="OHN27_001707"
     CDS             36665..37333
                     /locus_tag="OHN27_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCQ2286161.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(37351..37857)
                     /gene="tnpA"
                     /locus_tag="OHN27_001708"
                     /pseudo
     CDS             complement(37351..37857)
                     /gene="tnpA"
                     /locus_tag="OHN27_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            37865..>38380
                     /locus_tag="OHN27_001709"
     CDS             37865..>38380
                     /locus_tag="OHN27_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2286162.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTP"
BASE COUNT         9908 a         8777 c         9925 g         9770 t
ORIGIN      
        1 ggaaagtatg tttgattagg taataatcta ctggcaatat aggatgtctt caatgtttta
       61 aataactaat tggtcgggtt agtgcatccg gctttcttta tattcgccag aaggatttat
      121 tatgcaaagg aaaactctat tgtcggcctg tattgcatta gctctgagtg gtcagggttg
      181 ggcggcagat atcacagagg tagaaaccac cacaggtgaa aagaaaaata ccaatgtgac
      241 ttgtccggca gacccaggaa aactcagtcc ggaagagctt aaacgcttac cctctgaatg
      301 ctctccttta gtcgaacaaa acctgatgcc atggcttgcc acaggcgctg ctgcgttaat
      361 cacggcctta gccgtagtgg aactaaacga cgatgatgat catcatcatc gcaacaattc
      421 tccactccca ccgacacccc ctgatgatga atcagacgac actccagttc ccccaagtcc
      481 tggcggagat gagataatac cggacgatcc ggatgatacg cctacacctc ccaaaccgat
      541 ttcgtttaat aatgacgtta ttctcgataa agcagaaaaa acgttaacta ttcgcgattc
      601 agtttttact tataccgaga atgctgacgg gactatttct ctgcaagata gcaatggtcg
      661 taaggcaacg attaatcttt ggcagattga tgaagcgaat aacactgttg cccttgaagg
      721 ggtgagcgca gatggcgcaa cgaagtggca atataatcat aaaggtgagc ttgttattac
      781 gggtgataat gccacagtaa acaacaatgg caaaaccacc gttgacggca aggattccac
      841 cggtacggaa atcaacggta ataacgggaa agtgattcag gacggcgatc tggatgtcag
      901 cggcggcggt cacggtattg atatcaccgg tgacagcgcg acggtagata acaagggcac
      961 catgaccgtt accgatccgg agtccatcgg tatccagatt gacggcgaca aggcggttgt
     1021 taataacgaa ggcgagagca ccatcaccaa cggcggcacc ggcacgcaga ttaacggtga
     1081 cgacgccacg gcgaataaca gcggcaaaac caccgttgac ggcaaggatt ccactggcac
     1141 ggaaatcaac ggcaataacg ggaaagtgat tcaggacggc gatctggatg tcagcggcgg
     1201 cggtcacggt attgatatca ccggcgacag cgctacggtg gataacaagg gcaccatgac
     1261 cgtcaccgat ccggagtcca tcggtatcca gattgacggc gacaaggcca tcgtcaataa
     1321 cgaaggcgac agttccatca ccaacggtgg caccggcacg cagattaacg gtgacgaggc
     1381 cacgacgaat aacaacggca aaaccaccgt tgacggcaag gattccaccg gtacagaaat
     1441 caacggcaat aacggaaacg ttattcagga cggcgatctg gatgtcagcg gcggtggtca
     1501 cgggattgat atcaccggcg acagcgcgac ggtggataac aagggcacca tgaccgtcac
     1561 cgatccggag tccatgggta tccagatcga cggtgacaag gccatcgtca ataacgaagg
     1621 cgagagcacc atcaccaacg gtggcaccgg cacccagatt aatggtgatg acgccacggc
     1681 aaacaacaac ggcaaaacca ccgttgacgg caaggattcc accggtacgg aaatcaacgg
     1741 taataacgga aaagttattc aggacggcga tctggatgtc agcggcggcg gtcacggtat
     1801 tgatatcacc ggcgacagcg caacggtgga taacaagggc accatgaccg tcaccgatcc
     1861 ggagtccatc ggtatccagg ttgacggcga ccaggcggtt gttaacaacg aaggcgagag
     1921 cgccatcacc aacggtggca ccggcacgca gattaacggt gatgacgcca cggcaaacaa
     1981 caacggcaaa accaccgttg acggcaagga ttccaccggt acggaaattg ctggcaataa
     2041 cgggaaggtg attcaggacg gcgatctgga tgtcagcggc ggcggtcacg gtattgatat
     2101 caccggcgac agcgcaacgg tggataacaa gggcaccatg accgtcaccg atccggagtc
     2161 catcggtatc cagattgacg gcgaccaggc catcgtcaat aacgaaggcg agagcactat
     2221 caccaatggc ggcaccggca ctcagatcaa cggtaacgac gccaccgcga ataacagtgg
     2281 aaaaaccact gttgatggaa aagattccac gggtaccaaa atcgcgggca atatcggcat
     2341 tgtaaatctg gatggtagcc tgactgttac aggcggtgcg catggtgttg agaacattgg
     2401 tgacaacggc acggttaaca acaaaggaga tattgttgtt tccgatactg gatcgattgg
     2461 cgtgctcatc aacggtgagg gggcaacagt atccaatacg ggtgatgtta acgttagcaa
     2521 tgaagcgaca gggttcagca tcacaaccaa cagtgggaag gtttcgctgg caggcagtat
     2581 gcaggttggc gatttctcga ccggggtaga tcttaatggc aacaataaca gcgtgacgct
     2641 ggcggcaaaa gatctaaaag tggtcgggca gaaagcgacg ggcataaacg tttctggcga
     2701 tgcgaataca gtgaatatca ctggtaacgt tctggttgat aaggataaaa ccgcagacaa
     2761 tgcggcggaa tatttcttcg atccatccgt gggtatcaac gtttacggca gtgataataa
     2821 cgtgacgctg gatggaaagt taactgttgt atcagacagt gaggttactt ctcgtcagag
     2881 taatttattt gatggcagcg cagagaaaac gtcaggtctg gttgtgattg gcgatggcaa
     2941 taccgttaat atgaatggtg gacttgaact gattggagag aaaaacgcgc ttgcagatgg
     3001 gtcgcaggtt gcttccttgc gcacaggata tagttatacc agcgttattg tcgttagtgg
     3061 tgagtcgtcg gtatatctga atggagatac gacaatcagc ggagaattcc ctctggggtt
     3121 tgccggggtt attcgggtac aggataaagc tttgctggaa attggcagtg gcgctacgct
     3181 aacaatgcag gatattgaca gttttgaaca tcatgggaca agaaccccag aacttactta
     3241 tgctgattcc ggtgcgaaaa ttgttaataa aggtactgtt gaaattcaga atttaggttt
     3301 tgcttttgtt actggtgaaa atacaacagg tataaatagt ggcacgatct cgttattaca
     3361 aaatggtaaa gatccggcac cgtctcccat tgttttactg gctactaacg gagggagcgc
     3421 cactaatgca ggtacgatca caggtaaagt gacggaacaa catagcgtat ttaacaagta
     3481 ttcaacgggc acatcgaatt catttatttt taataacgat gtcagtagca taacagggtt
     3541 agtcgctcaa tcgaatagca caattatcaa tactgacagc ggcatcattg atttgtatgg
     3601 tcgtggtagt gtcggcatgc ttgctatagc agattcaaca gcagaaaatc agggtaaaat
     3661 tacactggat tctatgtggg tagatgcaaa tgacactacc gcaatgcgag atatagctag
     3721 caacagcgcc attgacttcg gtacaggtgt gggagttggt actgatagtt atagtggtgc
     3781 agggaaaaat gcaacagcaa ttaaccaatt gggcggtgtt ataactattt ataacgccgg
     3841 cgcaggtatg gcggcctatg gcgccagcaa tacagttatt aaccagggga cgattaacct
     3901 cgaaaaaaat ggtaattatg acgatagtct ggcagcaaat actctggtag ggatggctgt
     3961 ttatgagcat ggtactgcta tcaacgacca gacgggtgtt atcaatatca atgttggtac
     4021 tggtcaggcg ttttataacg atggcacagg aacaattgtt aactatggta caatctgcac
     4081 tttcggcgtg tgccaatcgg ggaatgagta caataataca gatgatttca cctcactgat
     4141 ctataccggt ggcgatacga ttacacgaag cggagaaact gtaacgctaa ataaatctgc
     4201 tgctgtgact gataagctgg ctgggaatgt tgttaatagc ggaacgcttt ccggtgatca
     4261 aattacggta tcaagcggtc ttctggaaaa taccagcggt ggcatcatca ataacttagt
     4321 aaaacttgac aagggtgccg tcattaaaaa tgccggggtg atgacgaata acgtcgatgt
     4381 tagcggtgga atcctcaata atgccggaga aatgactgcg caaattacca tgaatgctgg
     4441 tgctgatagt tcgttagtga acaacaccgg aaccatcaat aaaatcgtgc agaacgcggg
     4501 ggtattcaat aatagtggca gtgtaacagg gcggatgatg tcggctggcg gggtctttaa
     4561 taatcaaact gacggggcga ttatgagagg tgctgcgctg acaggtactg cagtggcaaa
     4621 taacgaagga acctggaacc tcggaagtag tagtgagggt aacaacaccg ggatgctgga
     4681 agttaataat aattcagctt tcaataaccg cggcgagttt attcttgata acgacaagaa
     4741 tgctgtgcac atcaaccagt ccggtacgct ttataatacc ggtcacatga acatcagtaa
     4801 ttcttcccac aacggagccg ttaatatgtg gggcggaaat ggtcgtttta tcaatgacgg
     4861 aacgattgat gtttctgcga agtcactggt agtcagcgct aataatgccg gcgatcagaa
     4921 tgccttcttc tggaaccagg ataacggggt catcaacttc gatcacgaca gcgccagtgc
     4981 cgtgaaagtc acccacagca actttattgc ccagaatgac ggcatcatga acatcagcgg
     5041 caccggtgct gtggctatgg aaggtgataa gaacgcgcag ctggttaaca atggcaccat
     5101 caacctcggt accgcaggca ctactgacac gggtatgatc ggtatgcaac tcgatgccaa
     5161 cgccacggcg gatgcggtaa tcgaaaacaa cggcaccatc aatatcttcg ccaatgactc
     5221 gtttgcattt agcgtactgg gtacagtagg tcatgtggtt aacaacggca cggtggtgat
     5281 tgccgatggg gttacgggtt ctggactgat caagcagggc gacagcatca atgttgaagg
     5341 tatgaacggt aacaacggta atagcagcga agtgcattat ggcgactata cgttgccgga
     5401 tgtgccgaag cccaatacgg ttagtgtaac gtcgggaagt gatgaggctg gtggcagcat
     5461 gaacaacctc aacggctatg tcgtcggtac caacgttaac ggcagcgccg ggaagctgaa
     5521 ggttaacaat gccagcatga acggcgtgga gattaacacg ggctttaccg ctggtacggc
     5581 agacaccact gtgagttttg ataacgtagt ggaaggtagc aacctgaccg acgctgacgc
     5641 catcacctca acgtccgtgg tatggactgc caaaggcagc accgatgcca gcggtaacgt
     5701 tgacgtcacc atgagcaaaa atgcctacac cgatgtggca acagatgcct cggtgaatga
     5761 catcgcgaaa gcactggatg cgggttacac caacaacgaa ctgtttacca gcctgaacgt
     5821 cggcacgact gctgaactga acagtgctct gaaacaggtc tgcggtagcc aggcgaccac
     5881 ggtattccgc gaagcgcgcg tgttaagcaa ccgctttagt atgctggcag atgccgcgcc
     5941 gaaagtgggt aacggtctgg cgttcaacgt tgtcgcgaaa ggcgatccgc gtgccgagtt
     6001 aggtaataat accgaatacg acatgctggc attgcgtaaa actatcgacc tgagcgaaag
     6061 ccagacgatg agtctggagt acggtatcgc tcgtctcgat ggtgatggtg cgcagaaagc
     6121 gggtgataat ggcgttacag gcggttatag ccagtttttt ggcctgaaac atcagatgtc
     6181 gttcgataac ggcatgaact ggaataacgc cttgcgttac gacgttcaca accttgacag
     6241 cagccgctcg attgcatttg gcaacacgaa caaaacggct gataccgacg tgaaacagca
     6301 gtacctggag ttccgcagcg aaggggcgaa gactttcgaa ccgagcgaag gactgaaggt
     6361 tacgccatat gcgggtgtaa aactgcgtca cacactggaa ggtggctatc aggagcgcaa
     6421 tgccggagac tttaacctga atatgaacag tggcagcgaa acggcggtgg acagcatcgt
     6481 cgggctgaaa ctggactacg caggtaaaga cggctggagc gctagcgcta cgctggaagg
     6541 cgggccgaac ctgagctacg cgaagagcca gcgtacggca agcctggcag gcgcaggcag
     6601 tcagcacttt aacgtcgatg acggtcagaa gggcggcggc atcaatagcc tgacaagcgt
     6661 cggcgtgaag tacagcagca aagaaagttc gctgaatctg gatgcgtaca actggaaaga
     6721 ggatggcatc agcgataaag gcgtgatgct gaacttcaag aaaacgttct aatttttagc
     6781 atgtgatccc taaaccgcaa cgctgataca ggttgcggtt tttttattgc cggatgtggt
     6841 acgtgacgcg ttttgttttg tgtctttcag gacaatagag caactcatcc agtaatcttg
     6901 tttacacctt cgcattattt atctcttttc gtttctatac tgatttttct taatccgttt
     6961 tattacaggg cagggtgcga tgagcagcaa tacatttact ctcggtacaa aatccgttaa
     7021 ccgtcttggt tatggcgcga tgcaactggc aggtcctgga gtttttggcc ccccacgaga
     7081 tcgccacgtc gctataaccg tgctgcgtga ggcgctggca ttgggcgtca atcatattga
     7141 taccagcgac ttttatggtc cgcacgtcac caatcagatt atccgcgaag cgctttatcc
     7201 ttactctgac gacctgacaa ttgtcactaa aattggtgcg cggcgtggag aggacgcatc
     7261 ctggttgccc gcattttctc cggcagagct gcaaaaagcg gtgcacgata atctacgtaa
     7321 tctcgggctg gacgtgctgg atgtggttaa cctgcgcgtt atgatggggg atggtcatgg
     7381 cccagcggaa ggatcgattg aggccagcct gaccgtgctg gcagagatgc aacaacaagg
     7441 cctggtaaaa catattggcc tgagcaacgt cacaccgacg caggttgcag aggcgcgcaa
     7501 gattgccgaa attgtctgtg tgcaaaacga atacaaacat cgcgcaccgt gctgatgatg
     7561 caatgattga tgctttggcc cacgatggca ttgcctacgt gccgttcttc ccgctcgggg
     7621 gctttacacc gctgcaatcg tccacacttt ccgatgttgc tgcgagcctg ggtgcaacac
     7681 caatgcaggt ggcgctggcg tggctgttac agcgttcacc gaatattttg ctgatcccag
     7741 ggacgtcttc ggttgcgcat ttacgggaga atatggctgc tgaaaaattg caactttctg
     7801 aggaagtgtt gtctacgttg gatggtattt cgcgagaata acgaatatac aaaagggaaa
     7861 gatgcatttc ccttttgttt cttttttaat ggcatggagt gcatatgttt aaggccagga
     7921 tttgcggctg gataggttta cttcccctgt ttatgctgag tcttccagta caggcggaac
     7981 ttcgatgtgt tgcaaatgca gttgatattg aacaattttt ttctgcggct accgctgaag
     8041 ataaacaaca agttgaacaa gctatcaaca gtagcgtgaa tcttgtcccc ttcggtttat
     8101 ctgcatcgga ctggaaagtg catcgtggcg atttagtggt agaaggtaat atagagagta
     8161 atcaaaaatt gattgttctt ggcaatttga cagtcaaagg aaatatttcc actttctctc
     8221 tttctaatcc atgggttatt ctcggtaacg tgacggcaac gaatatagtt acggattcac
     8281 cactgttaat taccggttcg ataaatgcaa gtggactggt atttatcgac tcatattacg
     8341 ataatccgtc tacgattaag gggagtatta atgcgcgtgg gatatttatc aatgacataa
     8401 ttgcacctgt tgttgcgtct tcgacaaata gtgaattcat ggtccgtgcg agtgacaaaa
     8461 atgacactga aaatgtcaaa aaagcgctga tgataataaa tcctgatgca tattattggg
     8521 gtctaataaa tgatgaagat gctctgaaag aaatttttaa gcgaagcaat attcgcatgg
     8581 cagggaatgt ctgtaatcag atgaaaaaag aagcgctgtt tcgccctaag ccttctcctg
     8641 agttagtgca ggaattgcaa atgctggatg aaggcaacgt tgctgcattt gaaggacgag
     8701 atattgcgac atttgatctt gccataatcc gaactctccc aaggcttaaa ggaatttcag
     8761 ctaacctacg caaacaactt attaatagca atgatgaaca aacgattgaa agtatggcga
     8821 gatatatgcc tgacaatgag attctggagt tgaccgatca gcaattgggt taccagcctg
     8881 ttgttctggg gttgctcgac cgtgaaccgc tctctgtcga aataatgacg cgaatgagtc
     8941 gtctacctga tggtgtcggt ccgttgaatc ttgcgctacg tgaaaatctc cctctggata
     9001 tcgtaatgac tctggcgaaa agagattggg atatgatcat tcaggagctt tataaagatg
     9061 cgtggttatt gcctgaatcc attattgatg gctatatccg cagtgatgat tcctctattc
     9121 gtcaggtcgg tgctggagga caactcacct acaatcaggc aatgcagttg gcgaacgatt
     9181 catcaaacaa tgttgtcaca agcttagcgt tcaagctggc agagatgaaa caccatggtc
     9241 aattattgcg gatgacgcca caagagagtg acaaagttgc agcctattta taccaaaaat
     9301 tcgagaatga cgatgatcta atacgtgtgt tatttttagc attgccagat aacttacagt
     9361 ttaattttgt taaaaggatg gagaaaaaat cccctgccta cttttgctgt cgggatatgc
     9421 aggtaattca ctctgacgct gctctacaac ggttattgac acgtttcaac gatcctgagg
     9481 ggtggagtaa tctggcgaaa aatcagtatc tgagcacgtc gatgaaacaa aaaatttggc
     9541 aacgtgcttt atcgcatcgg aaaaataatc cgaaagcaga ttcagctgcg tatgaaacca
     9601 gcgctgatat gattttgtct gagcttatca gccacggtga agttgatgat cagatgctat
     9661 taaatgccac agccctgata cgtttggaag actgggactt tttagagagt gcattagtta
     9721 gttgggataa tttacccgct gttgtcctca aggaattaca gcaaaatacg ccacgcaatg
     9781 atatttgggc gaagtttttt ctgaggcagg aaaacagctc ccgtgcgcag gttgatgaag
     9841 cattacgcgt ctattatgca ctcgaccccg atgcgttagc acaactggat gtactggcaa
     9901 aacaaccgga tcgtatatgg tggagtacac tggcgaaaag caatctcaca tttttcaagt
     9961 tcggcgcact taacaaccgc cacacaccgc ctgcagtact ggcggcagaa attgatcccg
    10021 agtggtggat tgtggcgatg aataatcccc gttttccagt tgatgtatta aaggcgaggc
    10081 tgaaacgtga tcctttgctg gcgttagagc ttgttaaccc tgaactggat ttagtccgcc
    10141 agctggcgct taacggtaag acgcgcgcaa tacgggaaca agcgatgaga aaacttgatg
    10201 agttgtattg atccacaacg taatatttct taaccctttg attaattggg gataattatt
    10261 cctgacagtg agtccccaat accttgatat attctgaatt tttaatgaag cggcgtgttg
    10321 cgatatctcc gtcaggggaa ttgatgcacc atagcgcaaa ccgaattatc aaggattgat
    10381 aatgacgctc taccagataa aaccgctctt tcagtcgctg ttaaggccga cgatgttttg
    10441 gctttacaag caccacgtta cagcgaatca catcactctt gctgcactgg cgctttctct
    10501 tcttaccgga ttgctgttga tgttggtggc acaacccatc ctctttatgc tattgcccat
    10561 cgtgcttttt atccgtatgg cgctcaatgc gctggatggc atgttggcgc gtgagtgcaa
    10621 tcagcaaaca cggcttgggg caattttgaa tgagactggt gatgttattt ccgatattgc
    10681 gctctattta acatttttat ttttaccgga aagtaacgca tcactcgtga tactcatgtt
    10741 attttgcacc atattgaccg agttttgcgg tttactcgcg cagacgatta acggtgttcg
    10801 cagttatgcc ggtccatttg gcaaaagcga tcgcgcgtta atatttggtc tgtggggtct
    10861 ggccgttgcc atttatccac agtggatgca gtggaataat cttttatgga gcattgcttc
    10921 aattctgctt ctctggactg cgattaatcg ttgtcggagt gtgcttctta tgagcgctga
    10981 aatataatgc tggaaaaatc tctggcaaca cttttcgcgt tgttaatttt agcaacgctg
    11041 ataaaccgtt ttctgctatg gcggttaccg gagagaaaag ggggtgaggt tacattacgt
    11101 attcgtacct ggtggggcat cgtcatttgt ttttcaatgg tgatttcagg tccacgctgg
    11161 atgacgttga cgttttttgc gctgataagt tttctggcat tgaaagaata ttgtacgatt
    11221 atatctgtac attttccgcg ttggttatat tggggtattc ctcttaacta tttgcttatc
    11281 ggttttaatt gctttgagct gtttctgtta tttatacctt tggctgggtt tctgatatta
    11341 gccaccgggc aagttttagt gggtgacccc tctggttttc tgcataccgt gagcgccatt
    11401 ttttggggct ggataatgac cgttttcgcc ttgagtcatg ccgcctggtt attaatgttg
    11461 ccaaccacaa atatccaggg aggggcgtta ctggtgttat ttcttttggc attaaccgag
    11521 tcaaacgata ttgcacagta tttatgggga aaatcctgcg gcagaagaaa agtggtccct
    11581 aaagtcagcc cgggaaaaac attggaaggt ctgatgggcg gcgtcatcac catcatgatt
    11641 gcgtcactga ttatcggacc gttactgaca ccgctaaata cattacaggc attattagcg
    11701 ggtttgttaa ttggtattag tggtttttgc ggcgatgtcg tgatgtcagc catcaaacga
    11761 gatattggtg ttaaagatag cggaaaacta ttgccaggac atggcggact ccttgacagg
    11821 attgactcat taattttcac cgctccggta tttttttatt ttatacgcta ctgctgttac
    11881 tgaaggaaaa tagagaaaat ggaaaattca cgcatccctg gggaacattt ttttaccacc
    11941 agtgataata cagcgttgtt ttatcggcac tggcccgctt tacagcccgg ggcgaaaaag
    12001 gtcatcgtct tatttcatcg cgggcatgaa cattctggtc gtctacaaca tctcgttgat
    12061 gaactggcga tgccagatac tgctttttat gcctgggatg cccgagggca tggaaaaagt
    12121 tcggggccgc gtggttatag cccatctctt gcgcgttcag tgcgggatgt cgatgaattt
    12181 gtccgttttg ctgccagcga cagccaggtt ggactggaag aggtggtagt gatcgcgcaa
    12241 agcgtcggcg cagtgctggt tgccacatgg attcatgatt atgcacctgc aattcgcggg
    12301 ctggtgctgg cttctccggc ctttaaggtt aaattgtatg tgccgctggc acgtcctgcg
    12361 ctggcgttat ggcatcgtct gcgtggtctg ttttttatta attcctatgt gaaaggacgc
    12421 tatttgaccc acgatcggca acggggggcg agtttcaata atgatccgct gatcacacgg
    12481 gcgattgccg ttaatatctt gctcgatctc tacaaaacgt ctgaacgtat tattagagat
    12541 gcggcggcga ttacgctccc cacgcaactt ctgatatcag gcgatgacta tgtggtgcat
    12601 cgccaaccgc agattgattt ttatcagaga ttacgtagcc ctctgaaaga gctgcatctg
    12661 ctgccaggct tttatcacga cacgttgggt gaagagaaca gggcgctggc atttgaaaaa
    12721 atgcaaagct ttattagtcg tttatatgct aacaaatcgc aaaaatttga ttatcagcat
    12781 gaagactgca caggaccatc agcggatcga tggcggctac tttctggtgg acccgtgcca
    12841 ttatcgccgg ttgatttagc gtatcgcttt atgcgaaagg cgatgaaatt gttcgggacg
    12901 cactcttcgg gcctgcatct cggaatgagc accggctttg attcaggcag ttcgctggat
    12961 tatgtctatc aaaatcaacc gcaaggtagt aacgcattcg ggcgtttagt cgacaaaatc
    13021 tacctgaaca gtgttggctg gcgcggtatt cgccagcgca aaacccattt acaaatactg
    13081 attaaacaag ccgttgccga tctccacgcc aaaggtttag ccgtccgcgt ggttgacatt
    13141 gccgcagggc atgggcgcta tgtactggat gcgctggcaa acgagcctgc cgtaagcgat
    13201 attttgttac gtgattacag cgagttaaat gttgcacagg ggcaagagat gattgctcaa
    13261 cggggaatgt ctgggcgggt gcgttttgaa cagggcgatg cgtttaaccc ggaggaactc
    13321 agcgcgttaa ctccgcggcc tacgctggcg attgtctctg gcctgtatga gctttttccc
    13381 gaaaatgagc aggtaaaaaa ctcactcgca ggtcttgcca atgccatcga accgggcggc
    13441 attctcctct acaccgggca gccgtggcac ccacaactgg agctgattgc cggggtgtta
    13501 accagtcata aagatggtaa accgtgggtc atgcgcgtgc gttcgcaaga ggagatggat
    13561 tcactcgtgc gtgatgccgg atttgataaa tgcacacaac ggattgatga gtggggtatt
    13621 tttacggttt cgatggcggt gcgtcgtgat aactgaacgt cggaacgtat tgctacaagg
    13681 cgctggctgg ttattgttgc tggccccgtt tttcttcttc acctatggat ctcttaatca
    13741 gttcaccgcg gttcaggacc ttaacagcca tgatatcccc agtcaggtat tcggttggga
    13801 aacggcgatc ccttttcttc cctggactat tgttccttac tggagtctgg atcttttata
    13861 tggattttcg ctgttcgttt gtagctcgac attcgaacag cgccgacttg tccaccggct
    13921 tattctggca acggtaatgg cctgctgcgg tttttttctc tatccgctga agtttagttt
    13981 tatccgtcct gaagtgagtg gggtgacagg atggctattt tcgcaacttg aactgtttga
    14041 tctgccttat aaccagtctc cttcgctgca tattattctc tgctggctac tttggcgtca
    14101 ctttcgtcag catctggctg tgaggtggcg taaagtctgc ggcggatggt ttttactcat
    14161 cgccatttcg acgctgacga cctggcagca tcattttatt gatgtcatca cggggctggc
    14221 ggtaggtatg ttaattgact ggatggtgcc cgtcgaccgt cgttggaatt atcagaaacc
    14281 tgatcaacgt cgaatcaaaa tagcactgcc atatgtcgta ggcgcgggct cgtgcattgt
    14341 gttgatggag ctaatgataa tgcttcagtt atggtggtca gtctggttat gttggccagt
    14401 attatcgcta ttcatcattg gccgtgggta cggtgggctt ggcgcgataa caacagggaa
    14461 agatagtcag gggaaactcc cgcccgccgt ttactggctg acattgccct ggcgtatcgg
    14521 gatgtggctg tctatgcgtt ggttttgtct tcgcctggag ccggtgagca aaattactgc
    14581 tggtgtttat ttaggggcgt ttccacgaca tattccggca cagaatgcgg ttctggacgt
    14641 cacctttgaa ttccctcgcg gacgagccac aaaagatcga ctctattttt gtgtaccgat
    14701 gctggatctg gtggttccgg aagaggggga gctccgacag gccgtggcga tgctggaaac
    14761 attacgcgaa gagcaaggca gcgttctggt ccattgtgca ttgggattat cgcgcagtgc
    14821 gctggtggcg gcggcatggt tgttatgtta cggacactgt aaaaccgtta atgaagcgat
    14881 tagctatatt cgagccagac gcccgcagat tgtgctgaca gacgagcaca aagcgatgct
    14941 gagattatgg gaaaacaggt aagtggattg agatgtggac tgaatatcta cagtccacat
    15001 caagaccgtg tccggttatg cagaaacaat gctgtcgatg gctgcttttg cgtcagactg
    15061 tgctttcgct gccatttccg gaccgtatgc gatcccttcg gcgaagacaa atttcacatc
    15121 ggtaatgccg ataaagccga ggaacgtgga cagatacggc gtcaccaggt ccgttggtcc
    15181 atctttgtgg atcccgccgc ggctggtaat aacgatggct tttttacccg ttaccagacc
    15241 ttccggaccg ttctcggtat agcggaaagt aacgcctgca cgtgcaacca ggtcaaaata
    15301 gtttttcagc tgagtcgaga tgttgaagtt gtacatcggt gccgcaataa cgataacgtc
    15361 gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc agagcttcct gctgacgcgg
    15421 agtcagcggc gcatcgctcg gacgcagtgc accaaccagt tcgccatcca gtaccggaat
    15481 cggatttgca gccaggtcgc gaacggtgat ttcatcagcg gagtgctttt cgcgccattg
    15541 ttcaacaaaa taatcggaca actgattaga ctgagagtac cctgccagga tgctggattt
    15601 aagaactaat accttgctca tggtgtttcc ttatagatat ttgaatgggc gatgccccgt
    15661 tgcttgttga cactttattc acaatcctgc cacagagata gcgcaataaa tcgaagccta
    15721 tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt ggtactctat atctatcatt
    15781 taaaagaaaa ttaatcaggc agcctacagc cctctaacgt tatgacagaa caacaaaaat
    15841 tgacctttac agccttgcag cagcggctgg attcgctgat gctgcgtgac agacagcgtt
    15901 ttttacgccg tctgcacggt gtgaagaagg ttaaaaatcc tgatgcacaa caggccattt
    15961 tccaggagat ggctaaagag attgaccagg cggcagggaa agtcctgctg cgtgaagcgg
    16021 cacgaccgga aattacttat cctgacaatt taccggttag tcagaaaaag caggacattc
    16081 tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg ggaaaccggt tccggtaaaa
    16141 cgactcagct accgaaaatc tgtatggagc tggggcgcgg gattaaaggg ctgatcggcc
    16201 atacccagcc gcgtcgtctg gcggcaagaa cagtggcgaa ccgtattgcg gaagagctga
    16261 aaacggagcc gggcggttgc atcggttaca aagtgcgttt cagcgatcac gtaagtgata
    16321 acacgatggt caagctgatg accgacggta tcctgctggc ggagatccag caagaccgcc
    16381 tgctgatgca gtacgacact atcattattg acgaagcgca cgaacgcagc ctgaatatcg
    16441 attttttgct cggctatttg aaagagttgc tgccgcggcg tcctgaccta aaaatcatta
    16501 tcacttccgc gactatcgac ccggaacgct tttcgcgcca ctttaataat gcgccgatta
    16561 ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta tcgcccgatt gttgaagaag
    16621 ccgatgacac cgagcgcgat cagttgcagg cgatttttga caccgtagac gaactgagtc
    16681 aggaaagcca tggcgacatt ctgatcttta tgagcggcga gcgggaaatc cgcgataccg
    16741 ccgatgcgct gaacaagctg aacttacgcc ataccgaaat cttgccgctt tatgcgcggc
    16801 tttcgaacag cgaacaaaat agggtattcc agtcgcacag cggacggcgc attgtgctgg
    16861 cgaccaacgt cgcggaaacg tcgctgaccg taccggggat taaatacgtt atcgaccccg
    16921 gtacagcgcg tatcagccgc tacagctatc gcaccaaagt gcagcgtttg ccgattgagc
    16981 cgatttccca ggcgtctgcc aatcagcgta aaggccgctg tggtcgtgtg tccgaaggga
    17041 tctgtattcg tctctattcc gaagacgatt tcctctcgcg cccggagttt accgatccgg
    17101 agattctgcg taccaacctg gcctcggtta ttttgcagat gaccgcgctg gggctgggcg
    17161 atatcgctgc gttcccgttt gtcgaagcac cggataaacg caatatccag gatggcgtgc
    17221 gtctgctcga agagctgggc gcgatcacca ctgatgaaca ggccagcgcc tataaactga
    17281 cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc acgtctggcg cgtatggtgc
    17341 tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat tatcacgtcc gcgctctcca
    17401 ttcaggatcc gcgcgaacgt ccgatggaca aacagcaggc atcggacgaa aaacatcgtc
    17461 gcttccacga caaagagtct gactttctcg cgtttgtgaa tctgtggaat tatcttggcg
    17521 agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct gtgtcgtacc gattatctca
    17581 actatctgcg cgtgcgcgaa tggcaggata tctacaccca gttgcgtcag gtggtgaaag
    17641 aacttggcat tccggttaac agcgaaccgg cggagtatcg cgaaattcac attgcgttgc
    17701 tgaccggttt gctttcccat atcggcatga aagatgccga taaacaagaa tataccggcg
    17761 cacgtaacgc gcgtttctcc atcttccccg gttctggttt attcaaaaaa ccgcctaaat
    17821 gggtaatggt ggcggaactg gtagaaacca gccgcctgtg ggggcgcatt gctgcgcgta
    17881 tcgacccgga atgggtggag ccagttgctc agcatttgat taaacgcacc tacagcgaac
    17941 cgcactggga acgggcgcag ggcgcggtga tggcaacgga aaaagtcact gtttatggtt
    18001 tgccgattgt tgctgcgcgt aaggtcaact acagccagat cgatccggcg ttatgtcgtg
    18061 aactctttat tcgccacgct ctggtggaag gtgactggca gacgcgtcac gcattcttcc
    18121 gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga acacaaatca cgtcgccgcg
    18181 atattctggt tgatgacgaa acgctgtttg agttctacga ccagcgcatc agccacgatg
    18241 taatctccgc tcgtcacttc gatagctggt ggaaaaaagt cagccgcgaa acgcctgatt
    18301 tgctcaactt tgaaaagagc atgttgatca aagaaggggc ggaaaaaatc agcaagctgg
    18361 attacccgaa cttctggcat cagggcaatc tcaagctgcg tttgagctat cagtttgagc
    18421 caggcgcaga cgcggacggt gtgaccgtac atattccgct gccgttactt aaccaggttg
    18481 aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg cgaattggtg attgctctga
    18541 ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc tgcaccaaac tacgccgaag
    18601 cgtttttagg ccgcgtcaca ccgctggagt taccgttgct cgacagcctt gagcgcgagt
    18661 tacggcggat gaccggcgtt accgttgacc gcgaagactg gcactgggat caggtgcccg
    18721 atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa caagaagcta aaagaagggc
    18781 gctcgctaca agatctgaaa gatgcgctga aaggcaaagt gcaggaaacg ctgtctgcgg
    18841 tggcggatga cggtatcgag cagagcgggc tgcatatctg gagttttggt cagctgccgg
    18901 aaagctacga acagaagcgt ggcaactaca aagtgaaggc gtggccagcg ctagtggatg
    18961 aacgcgacag tgtggcgatc aaactgtttg ataatccgct agagcaaaaa caggcaatgt
    19021 ggaacggtct tcgccgtcta ctgctgctga atattccctc gccgatcaag tatttacatg
    19081 agaagttacc aaacaaagcc aagctggggc tgtactttaa cccgtatggc aaagtgctgg
    19141 agctgatcga cgactgtatc tcctgcggtg tggataaatt gatcgacgcc aatggtggcc
    19201 cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa agtgcgtgcc gaactgaacg
    19261 acacggtggt ggatattgcg aagcaggtcg agcaaatcct tacggcagtg ttcaatatca
    19321 acaaacgtct gaaagggcgg gtggatatga ccatggcgct ggggctttct gacattaaag
    19381 cgcagatggg cgggttggta tatcgcggtt ttgtcactgg taacggcttc aaacggctgg
    19441 gcgacacgct gcgatatttg caggcgattg aaaaacgact ggaaaaactg gcggttgatc
    19501 cacatcgcga ccgtgctcag atgctgaaag tcgaaaacgt ccagcaggcg tggcagcaat
    19561 ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga cgtgaaagag atccgttgga
    19621 tgatagaaga gctgcgcgtt agttacttcg ctcaacaact tggtacgcct tatccgattt
    19681 cagataagcg tattttgcag gcgatggagc agattagcgg ttaaccctgc tatttgcctg
    19741 ataaatctaa aacccggtaa gcatttagcg ccgggttttt tattaattct aaaacggtaa
    19801 ggacaaaaat tcaggaattc agaaaaatac aattctcagc tgcaagatga ataatgttta
    19861 tctacagcat ttccttaaaa gatatgtcag gcttgcggag tggcggttaa ggaaatacga
    19921 cttcctcctt tcagagtgct ccgcttctca ctattatctc acgcagtatt cttaagggaa
    19981 cgataaggag gaaccatgaa cattaccccg tttccgacgc tttcgacggc aactatagat
    20041 gccataaatg ttatcggaca gtggctggcg caggatgatt tctccggtga ggtgccgtat
    20101 caggctgatt gcgtcatact tgcaggaaat gcggttatgc cgactatcga tgcggcatgt
    20161 aagattgctc gcgatcagca aattccttta ctgattagtg gtggtatcgg tcactcgaca
    20221 acttttttgt atagcgccat cgcacagcat ccgcactaca acactatccg caccactggc
    20281 agagcagaag cgaccatcct ggcggatatc gctcatcagt tctggcacat tccgcatgaa
    20341 aaaatctgga ttgaagacca gtcaacaaac tgcggtgaaa acgcacgctt tagcatcgcg
    20401 ctattgaatc aggccgtaga acgagttcat acggctatcg ttgttcagga ccccaccatg
    20461 cagcggcgca cgatggcgac gttccgccgt atgactgggg acaatcccga tgcaccacgc
    20521 tggttaagtt atcccggatt cgttcctcag ttaggaaata acgcagacag tgtaatcttt
    20581 gttaacccgt tgcaaggatt atggccggtt gagcgttatc tatcactact cactggcgag
    20641 ctgccgcgtt tacgcgatga tagcgatggc tacggtcccc gcgggcgaga ttttatcgtt
    20701 cacgttgatt ttccggcaga agtcatccat gcatggcaaa cgctgaaaca cgatgcggtg
    20761 cttatcgagg cgatggaaag tcgctcgtta cgttaaaaat tgcccgtttg tgaaccactt
    20821 gtttgcaaac gggcatgact cctgactttt atttctgcct tttattcctt ttacacttgt
    20881 ttttatgaag cccttcacag aattgtcctt tcacgatgcc gtctctctga tgattgatgt
    20941 taattaacaa tgtatttacc gaaaacaaac atataaatca caggagtcgc ccatgtcagt
    21001 acccgttcaa catcctatgt atatcgatgg acagtttgtt acctggcgtg gagacgcatg
    21061 gattgatgtg gtaaaccctg ctacagaggc tgtcatttcc cgcatacccg atggtcaggc
    21121 cgaggatgcc cgtaaggcaa tcgatgcagc agaacgtgca caaccagaat gggaagcgtt
    21181 gcctgctatt gaacgcgcca gttggttgcg caaaatctcc gccgggatcc gcgaacgcgc
    21241 cagtgaaatc agtgcgctga ttgttgaaga agggggcaag atccagcagc tggctgaagt
    21301 cgaagtggct tttactgccg actatatcga ttacatggcg gagtgggcac ggcgttacga
    21361 gggcgagatt attcaaagcg atcgtccagg agaaaatatt cttctgttta aacgtgcgct
    21421 tggtgtgact accggcattc tgccgtggaa cttcccgttc ttcctcattg cccgcaaaat
    21481 ggctcccgct cttttgactg gtaataccat cgtcattaaa cccagtgaat ttacgccaaa
    21541 caatgcgatt gcattcgcca aaatcgtcga tgaaataggc cttccgcgcg gcgtgtttaa
    21601 ccttgtactg gggcgtggtg aaaccgttgg gcaagaactg gcgggtaacc caaaggtcgc
    21661 aatggtcagt atgacaggca gcgtctctgc aggtgagaag attatggcga ctgcggcgaa
    21721 aaacatcacc aaagtgtgcc tggaattggg tggtaaagca ccagctatcg taatggacga
    21781 tgccgatctt gaactggcag tcaaagccat cgttgattca cgcgtcatta atagtgggca
    21841 agtgtgtaac tgtgcagaac gtgtttatgt acagaaaggc atttatgatc agttcgtcaa
    21901 tcggctgggt gaagcgatgc aggcggttca atttggtaac cccgctgaac gcaacgacat
    21961 tgcgatgggg ccgttgatta acgccgcggc gctggaaagg gtcgagcaaa aagtggcgcg
    22021 cgcagtagaa gaaggggcga gagtggcgtt gggtggcaaa gcagtagacg ggaaaggata
    22081 ttattatccg ccgacattgc tgctggatgt tcgccaggaa atgtcgatta tgcatgagga
    22141 aacctttggc ccggttctgc cggtggtcgc atttgacacg ctggaagatg ctatctcaat
    22201 ggctaatgac agtgattacg gcctgacctc atcaatctat acccaaaatc tgaacgtcgc
    22261 gatgaaagcc attaaagggc tgaagtttgg tgaaacttac atcaaccgtg aaaacttcga
    22321 agctatgcaa ggcttccacg ccggatggcg taaatccggt attggcggcg cagatggtaa
    22381 acatggcttg catgaatatc tgcagaccca ggtggtttat ttacagtctt aatgagtgaa
    22441 agaggcggag gttttttcct ccgcctgtgc gcgtcagagt ttagcgaatt tttcgagggt
    22501 gcgaataagc tgtgtgacga agccatattc gttatcgtac caggcgaccg ttttcaccag
    22561 ttgtaaatcg cccacggcgg taatttccgt ttgcgtggca tcaaacaccg aaccgaaatg
    22621 gctgccaatg atatcggaag agactatttc ttcatcggta taaccaaatg actcgttatt
    22681 ggtggttgct tgtttaagtg cgttattcac ctcttcggca gtcacttttt ttccgagaat
    22741 cgataccagt tcagtgaccg aacctgtttt caccggcacg cgttgcgcat gacctttcag
    22801 tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg tagtgtgggg
    22861 aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg ggccatccac
    22921 cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt ctatcccgaa
    22981 actgtcatgc aaggctttgg ccatcggcgc aagacagtta gtggtgcatg acgccacgga
    23041 aacaatggtg tcgttgccat ccagagtgtc gtcattgacg ttataaacga tagttttcat
    23101 ttcaccggca ggggcggaaa tcaacacctt cttcgcacca gcatcaagat gcgcctgcga
    23161 tttctcggcg gaggtataaa agccagtaca ttcgacaatg atttctgcac ctttcgcttt
    23221 ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc catcaacgat
    23281 aagtgaatct tccgtaaaat caacgctcca ggggaatggt ccgtagtttg aatcatgttt
    23341 cagcaggtag gcgagaattt ttggggaagt gagatcatta atagcgacaa cgtctatgtt
    23401 gcttttgact tcaagtaatc gacgcaacac cagtcgaccg atacgaccaa aaccgttaat
    23461 accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt gatcctttcc
    23521 aggcttagac caggatggcg ggatgggcaa tctccattct cacagtgaaa cgtaacgtac
    23581 tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg ttaacggaaa
    23641 attatgttaa agcaggaaat gttatggaaa ataaatattc aaggttacaa atcagcattc
    23701 actggctggt ctttttactg gttatcgcag cgtattgcgc aatggagttt cgtggtttct
    23761 tcccacgtag cgatcggcca ctcatcaaca tgattcatgt ttcctgtggc atctcaattc
    23821 tcgtgctgat ggtcgttcgt cttttgttaa ggctgaaata cccaaccccg ccgattatac
    23881 ctaagccaaa accgatgatg acgggactgg cgcatttggg acatttggtg atttatctcc
    23941 tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc aacccgtggt
    24001 ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg gtagatagct
    24061 taaagtcgtg gcatgaaacg ctggcgaatc tgggatattt tgtcatcggg ttgcacgctg
    24121 cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc atgatgccgc
    24181 gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac ccgtggtaca
    24241 ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat gacaaataaa
    24301 accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac ccgatctttg
    24361 atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgaac ataagattcg
    24421 ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg aacgcttgtt
    24481 tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg gatctcacac
    24541 tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc gaaaaaaaca
    24601 tgcactaact gaagtgtaaa aggggtgcca tgagataatg aatctgctag tcagatgcgc
    24661 ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc agcaacctcc
    24721 ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag tgtgaatgtc
    24781 agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgttt catagccttt
    24841 ctccttgccg gatggcgggt aagaggctaa gatctgaatt gcttggttca ttcgttggcc
    24901 tcggtttgta ctgaccccaa aaagttggac agttaaacac gaggcatata ggtctgattc
    24961 cgatattcaa ttggagtcag accttttaat ttcaggctaa ttcttctgct gttgtagtat
    25021 tcaatatatt ccgtaacagc atccttcagt tcgcttatat tactgaactc atcaagataa
    25081 aaacactccg actttaaggt tccaaagaaa cactccacca cagcattatc cagacaattg
    25141 ccttttctgg acatgctttg tttaatacca tgttctttaa ggatattttg atatcttctc
    25201 atacgatact gccatccctg gtcagagtgc agaacaggat gctcgtgagg attaagcttt
    25261 ttgaatgcct gatcgagcat attctcaacc atgttcatca ctggtctttc cgaaaggctg
    25321 taagaaataa cttcgttgtt gaagagatct attactggag acaaatacag cttgcgccca
    25381 ttgactgcaa attcagtaac atcggtaacc cacttctcgt ttggccgcgt agccttgaaa
    25441 tctctttgga gaacattagg ggcggtttgc cctacctctc ctctgtaaga gcggtatcgc
    25501 ttgaccttaa tcgctgcttt aagtgagagg gttcccatca ggcgctgaac agctttatgg
    25561 ttaatctgtt tcccttctcg atgaagagac agcgttaccc tacggtatcc gtatcggcct
    25621 ctattctcgt gataaatctc actaatacgc tttttaacgt ccgcatactt gtcaggcttg
    25681 ctgagagcct ttagatgata ataaaacgta ctgcgcggta tctccgcagc cctgagaagc
    25741 tcatcaagag gataaaactg ccttagctcg ttgagtactt tcactttttc gtgggatgag
    25801 ctaaggcttt cagctttttt agatacataa gccgcgtttc aagaaatcga acttgccttt
    25861 caagatcctc aatgcgtcgg tcttttgaca gctccaatgc tgatgccgct ttttctggat
    25921 caactgatat tgcaatgttt cttttggtgc caatcttgag cgcgcgtaaa ccagcttctc
    25981 cgcgctcttc atagaccttc agccacctgg ctacagaacc actaccagca agcataaagt
    26041 gagcagcagc ctgattaagg gacatgtgct gctcgatcac agctttcacg accttaatac
    26101 gcaactctgg atcagcacta acgcctttag gtttgggaat taaacctttt tctccatgtt
    26161 tttcatagag ggcaacccat gtcctgacct gggttcgggg gacaccaaaa cgtgccgaga
    26221 tgatcctgta accatcatca gttgtgaagt agtgattcac gacttcaagg cgcttttcaa
    26281 aagggtattt tggctttgac atattagggg ctattccatt tcatcgtcca acaaaatggg
    26341 tgcagtacat tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct
    26401 gaaaacagaa agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat
    26461 aatcacttca cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt
    26521 ttagccattc gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt
    26581 aaaaatgtga cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt
    26641 tctatcagga gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga
    26701 ttaggttttt tgcatcacat caggctggtt ccgttatttg cctgcattct aggcggtatc
    26761 ttagttctat tcgcattaag ttcagccctt gccggctatt tcctctggca ggccgatcgc
    26821 gatcagcgtg atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat
    26881 tttttgcgtt cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa
    26941 atggaagcaa tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa
    27001 ggttatcgtg cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cctcgacact
    27061 gaattaaatc aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc
    27121 aaaaatggca tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat
    27181 aatgcttata ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa
    27241 ctggcggaac tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt
    27301 gtgcttgccc tggtcatgac gctgataaca tttatggtgc taagtcggat cgtcattcgt
    27361 ccactgcaac atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat
    27421 gatgaaccgg cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag
    27481 cattcactgg ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc
    27541 atcagcgaaa tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg
    27601 gctatcgaac aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg
    27661 gataacgcgc atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc
    27721 gggcagacgg tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa
    27781 atttctgaga tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg
    27841 aatgctgccg ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc
    27901 agcgaagtac ggacactcgc aagtcgcagc gctcaggcgg cgaaagagat tgaaggcttg
    27961 atcagtgaat cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggccgggaaa
    28021 accatgagca ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc
    28081 gccgcctcgg atgaacaaag tagaggcata acgcaggtta gccaggcgat ttctgaaatg
    28141 gataaggtga cgcaacagaa tgcttctctg gtagaagagg cctcagcggc ggcggtgtcc
    28201 cttgaagaac aggcggcacg attaactgag gcggtggatg tattccgtct gcacaaacat
    28261 tctgtgtcgg cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa
    28321 tgttcaagga gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag
    28381 cgttaatcat caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa
    28441 tcggttcgcc tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt
    28501 cgtcttttac agcgccagaa gggacaaacc acacgtaatc gtattcaacc gtaagttgac
    28561 gagatagcga agcagacagc gtttcgatac aacccgaagg aattttacag ccctggctct
    28621 gcactaatgc atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc
    28681 attccagcac ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa
    28741 ccagcttcaa cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat
    28801 ctgacatccg accaatgccg atatcgattt ccccggtttt taaactcgcc agaatcatag
    28861 ggttactcat tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta
    28921 taaccgaagg taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat
    28981 tattaagacc ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc
    29041 tgactgcatg cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac
    29101 gctcaaacaa gcgagcgcca gtcagctgct ccagttcatt caatgtctta gagagcgcag
    29161 gttgactcaa attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta
    29221 cgaatgtatg aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa
    29281 gcgatgttaa aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg
    29341 attgcaaaat attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat
    29401 gttttcgcgg cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta
    29461 cactccagat tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga
    29521 gattcgggaa cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata
    29581 tggcacgctg ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca
    29641 actgcacgaa aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg
    29701 ggcgattcgc gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat
    29761 ggggatgtac ccggtgagct attacgatct ctcgcaggcg ggggtgccgg tacattcgac
    29821 agcatttcgg cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacctc
    29881 gttactccgc cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct
    29941 acgtcagcgc gatatcttca ccccacgttg tcgactactg ttagaggaat atgagcagcg
    30001 gggcggtttt aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg
    30061 ctggcaccag tcagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg
    30121 gttgattgct gatgtggtct gttttcctgg atgccatatc aaccatctga cgccacgtac
    30181 gctggatatt gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac caaaaattct
    30241 gatcgaaggg ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc
    30301 actggaagag acggtgttgt ttgcggggca gaaacagggc acgcatactg cgcgctttgg
    30361 tgaaattgag cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct
    30421 tctgcgtaac gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac
    30481 cttccgcact tttcctgaca gtgagttttt aatgcgtcag caagggctgg catggttccg
    30541 gtaccgtctg acgccttcgg gtgaggcgca tcgtcaggcg attcatcccg gagacgatcc
    30601 acagccctta attgaacgtg gttgggtcgc ggcgcaaccc attacctatg aagatttctt
    30661 gcccgttagc gcggcgggga tcttccagtc aaatctgggt aatgaaacgc aggcacgcaa
    30721 tcacggtaat gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga
    30781 gttccagctt tatcaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat
    30841 tctgaccatc cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca
    30901 tcgacataaa aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa
    30961 taaatattgt ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca
    31021 gcgcgggacc attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt
    31081 attaaaggtt caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt
    31141 tctcaggcgg cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac
    31201 ttctccattc tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct
    31261 cgtccgttac ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac
    31321 gacgccgaaa aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc
    31381 catatgggaa tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat
    31441 ctggcgcgtg aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat
    31501 accaaacaat tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc
    31561 cccgaactgg cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt
    31621 gatgatacat atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac
    31681 agtaaagaag agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca
    31741 actaccttta ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc
    31801 actatccatt gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc
    31861 aaagacatta aacagctggg cattgcgccg atgaccagta tgttcagctg cggtactaat
    31921 gaacgtcgga tgtgcgatac cattcatccg caaattcatg actctgatcg tctgtccatg
    31981 tggcggggca acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc
    32041 aatgcttaca ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc
    32101 tcccattatc aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg
    32161 cgtaacaagt ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg
    32221 ctggataaca ttgtctgctt ctggaagcca gaaaaagctg taaaagcagg tgatgagttt
    32281 gcattccagt atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc
    32341 gttatggcga cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac
    32401 tatcccgaaa aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct
    32461 gccgcgccaa aaggcattga gccggtgatt acgctgtcca gtggggaagc gaagcaaatc
    32521 gaaattctct atattgaacc cattgatggt tatcgtattc agtttgactg gtatccgacg
    32581 tcggactcca ctgatccggt cgatatgcgg atgtatctgc gttgtcaggg ggacgctatc
    32641 agtgaaacat ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac
    32701 gaccgcgtga tgagttaatt gttttttctt cggcaccttc ttcgggaggt gccgtctggt
    32761 taaacacgat cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt
    32821 tcagatacac cttgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt
    32881 aatatcccgt ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa
    32941 acttgatcat gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt
    33001 ggtccgcatg aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca
    33061 ggagagcgtc aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa
    33121 cagcatggat tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa
    33181 ttacatgctg ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa
    33241 acctggttac agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga
    33301 aaaacggtgc agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt
    33361 ttcaaagaag atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa
    33421 actgctgaaa tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag
    33481 gcgctgcagg cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc
    33541 aaatgtcggg tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt
    33601 gaaggttgcc tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac
    33661 gcgcgtatta tcggttcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc
    33721 gttaatgact tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc
    33781 gtggtcggtt aactccacat ggttttcaat tatcaccgca cctgaaatac ggctctcacc
    33841 ttgtattaca acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc
    33901 aatcaagaca tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg
    33961 cgaactgtaa tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg
    34021 accgtagact ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaatact
    34081 ggcaaaatca aaaacttcgg cgcgatgttc aataaaagca taccggatga cggcatcgcc
    34141 ataaatttgt gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg
    34201 tagcaggagt tgatggtcag gcgttaagcc ttgtgctgcg acgatcatag aatgttgatt
    34261 aattaaggcg tgaccaaata ttctacattg cccgcaaacc agtgagtcat gaatggtgac
    34321 actgtcactt atatatgcac cctggctgat ttcactatta tcgatccaga cattatccgt
    34381 tgcgtaaacc tctccccaca gtacgctggt gccggtgatg cgcgtattac cggaaatcac
    34441 cgttccgccg aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa
    34501 caccgtttcg cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct
    34561 aatggcgata atctgccgta ataacacatt ttttttagtg ccatcttcct gataactaaa
    34621 ggctcgctgt tcttcactta gacgatattt gcgcatagcg ttttcccaca ggtgacttac
    34681 tataaccgta gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt
    34741 tcaaatatac tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc
    34801 aatttgccgg caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg
    34861 cgctatgcca gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg
    34921 gcgatgatca tctggggatt attgactagc gcatttattg cccgactcat acgctttccg
    34981 catagcgtgc tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg
    35041 gcaacgacga tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc
    35101 ctgttcagct ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta
    35161 tggcgcggat ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc
    35221 aacaacttta tcagcgcaat ggcctgtggt gcgttgggtt acaccgacgc cggtctggtg
    35281 tttttaggcg caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg
    35341 cgtagttcgg gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct
    35401 gcgctggtgg cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg
    35461 aaaatgcttt tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg
    35521 tatctctccc agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg
    35581 gcaaccaccg gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg
    35641 gtgccgctgt ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg
    35701 cttctgatgc agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag
    35761 gacaaagaat gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca
    35821 cacactctga agtaatggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg
    35881 gctgtggcaa tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat
    35941 gggataaaaa tgccatgagt atcgccaacg tcgagcgcat taaatctatt gaaaatctgg
    36001 ataatttaca cactcgggtc gttgatctga ataacctcac atttgctgga cagtacgatt
    36061 ttattctttc gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggctgattg
    36121 ccaatatgca acgttgcacg aaacctggcg gttacaacct gattgtggcg gcgatggata
    36181 gcgctgatta tccatgtacc gtcggcttcc cgtttgcctt caaagagaga gaattacgtc
    36241 gatattacga aggctgggag atggtgaaat acaatgaaga cgtcggcgag ctgcaccgca
    36301 ccgacgccaa cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat
    36361 gacccggtaa gaacaaagcg cgtgaaaatt ccccacgctg agatagttta ctgttcttct
    36421 tttcggtaag catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt
    36481 ttagcagctt aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca
    36541 ttgactatgt cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca
    36601 caacacgaag cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact
    36661 attcatgcgt accacatcat ttgcgaaagt tgcagcttta tgcggcttat tggctctgtc
    36721 tggttgtgcc tctaaaatca cccagccaga taaatattct ggttttttaa acaattactc
    36781 tgatttaaaa gaaacaacct cggctacagg taaacctgtt ttacgttggg tagacccgag
    36841 ttttgatcaa agcaaatatg acagcatcgt ctggaaccca atcacttatt atccggtacc
    36901 gaaaccgtcg acccaggtag ggcagaaagt tctggataaa attttgaact ataccaacac
    36961 cgaaatgaaa gaagcgatag cgcagcgtaa accactggtt accaccgctg ggccgcgtag
    37021 tctgattttc cgtggggcca ttaccggtgt agataccagc aaagaagggc tgcaattcta
    37081 tgaagtggtt cctgttgcat tagtggttgc ggggacgcaa atggctacag gccaccgtac
    37141 catggatact cgcctctatt ttgaaggtga gctgattgat gcagcgacta ataaaccggt
    37201 tatcaaagtc gttcgtcagg gcgaaggtaa agacctgaat aacgaaagta cgccaatggc
    37261 tttcgaaaat attaaacaag ttattgatga catggcgacc gatgccacca tgtttgacgt
    37321 taacaaaaag tagttccaga cgcgccatcg ttagatggcg ctttttatcc ggtgcgccgt
    37381 aaaaccccat ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgtttg
    37441 ctgttgttca atgtattggc ggatgacgga tattggcgca cctccgcagc tactggcaaa
    37501 atagccagga ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc
    37561 tcgtcgcagt cacctacccg atacgccttt gaggctgttt accagactgg atatcgccag
    37621 tttgggagga tagttaatta acaaatggac gtgatctggt tcgccatcca tttcaaccag
    37681 ttcagcttca aaatcagcac atacatttga aaagtaagtg cgtagttttt ctgtcgcgtc
    37741 atggtcaaaa atctggcgtc ggtatctggt gacaaagagc aggtgaacat gcatcaggaa
    37801 aacacaatgc cttccacgcc ggatatcggt ttcttttttc acagaccaaa gtatcatttg
    37861 acctgtgaaa cgattacagg catttaaatt ccagttaaga ccaggtggtc aacaggagtg
    37921 tgaaatgagg cgcttcgcag gcgcatgtcg tttcgttttc aatcgtgctc tggcacgtca
    37981 gaatgagaat catgaggccg gtaataaata catcccttac gggaaaatgg cttcctggct
    38041 ggttgagtgg aaaaatgcca ctgaaacgca atggcttaaa gattctccct cacagccatt
    38101 gcaacagtca ctgaaagacc ttgagcgggc ttacaaaaac ttcttccgga agcgggctgc
    38161 ttttccccga ttcaaaaagc ggggacagaa tgatgcattc cgctacccgc agggcgttaa
    38221 gctcgatcag gaaaacagcc gtatttttct gccgaaactg ggctggatgc gctaccggaa
    38281 cagccgtcag gtcacgggtg ttgtgaaaaa tgtcactgtc agccagtcct gcggtaagtg
    38341 gtacatcagt attcagacag aaagtgaagt atcaactccg
//
LOCUS       DAJOSY010000031        37953 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJOSY010000031 DAJOSY010000000
VERSION     DAJOSY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.031"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            132..2375
                     /gene="pdeF"
                     /locus_tag="OHN27_001710"
     CDS             132..2375
                     /gene="pdeF"
                     /locus_tag="OHN27_001710"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCQ2286163.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMLVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2414..3955)
                     /gene="ppx"
                     /locus_tag="OHN27_001711"
     CDS             complement(2414..3955)
                     /gene="ppx"
                     /locus_tag="OHN27_001711"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCQ2286164.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3960..6026)
                     /gene="ppk1"
                     /locus_tag="OHN27_001712"
     CDS             complement(3960..6026)
                     /gene="ppk1"
                     /locus_tag="OHN27_001712"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCQ2286165.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6197..6835)
                     /gene="purN"
                     /locus_tag="OHN27_001713"
     CDS             complement(6197..6835)
                     /gene="purN"
                     /locus_tag="OHN27_001713"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCQ2286166.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6835..7872)
                     /gene="purM"
                     /locus_tag="OHN27_001714"
     CDS             complement(6835..7872)
                     /gene="purM"
                     /locus_tag="OHN27_001714"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCQ2286167.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8197..8823
                     /gene="upp"
                     /locus_tag="OHN27_001715"
     CDS             8197..8823
                     /gene="upp"
                     /locus_tag="OHN27_001715"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCQ2286168.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8909..10198
                     /gene="uraA"
                     /locus_tag="OHN27_001716"
     CDS             8909..10198
                     /gene="uraA"
                     /locus_tag="OHN27_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCQ2286169.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10248..10994
                     /gene="hda"
                     /locus_tag="OHN27_001717"
     CDS             10248..10994
                     /gene="hda"
                     /locus_tag="OHN27_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCQ2286170.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11132..11491)
                     /gene="arsC"
                     /locus_tag="OHN27_001718"
     CDS             complement(11132..11491)
                     /gene="arsC"
                     /locus_tag="OHN27_001718"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCQ2286171.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11512..12975)
                     /gene="bepA"
                     /locus_tag="OHN27_001719"
     CDS             complement(11512..12975)
                     /gene="bepA"
                     /locus_tag="OHN27_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCQ2286172.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13188..14249
                     /locus_tag="OHN27_001720"
     CDS             13188..14249
                     /locus_tag="OHN27_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCQ2286173.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14287..15135)
                     /locus_tag="OHN27_001721"
     CDS             complement(14287..15135)
                     /locus_tag="OHN27_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCQ2286174.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPPPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15157..17169)
                     /gene="hyfR"
                     /locus_tag="OHN27_001722"
     CDS             complement(15157..17169)
                     /gene="hyfR"
                     /locus_tag="OHN27_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCQ2286175.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESTLLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17199..17612)
                     /gene="hyfJ"
                     /locus_tag="OHN27_001723"
     CDS             complement(17199..17612)
                     /gene="hyfJ"
                     /locus_tag="OHN27_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCQ2286176.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIT"
     gene            complement(17605..18363)
                     /gene="hyfI"
                     /locus_tag="OHN27_001724"
     CDS             complement(17605..18363)
                     /gene="hyfI"
                     /locus_tag="OHN27_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCQ2286177.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFLVLRGLHD"
     gene            complement(18360..18905)
                     /gene="hyfH"
                     /locus_tag="OHN27_001725"
     CDS             complement(18360..18905)
                     /gene="hyfH"
                     /locus_tag="OHN27_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCQ2286178.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18915..20630)
                     /gene="hyfG"
                     /locus_tag="OHN27_001726"
     CDS             complement(18915..20630)
                     /gene="hyfG"
                     /locus_tag="OHN27_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCQ2286179.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20620..22200)
                     /gene="hyfF"
                     /locus_tag="OHN27_001727"
     CDS             complement(20620..22200)
                     /gene="hyfF"
                     /locus_tag="OHN27_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCQ2286180.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRRLSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGIYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFLLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22205..22855)
                     /gene="hyfE"
                     /locus_tag="OHN27_001728"
     CDS             complement(22205..22855)
                     /gene="hyfE"
                     /locus_tag="OHN27_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCQ2286181.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22867..24219)
                     /gene="hyfD"
                     /locus_tag="OHN27_001729"
     CDS             complement(22867..24219)
                     /gene="hyfD"
                     /locus_tag="OHN27_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCQ2286182.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRVSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQRSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPGVGFCVAALAITGVPPFNGFFSKFPLFAAGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(24195..24305)
                     /locus_tag="OHN27_001730"
     CDS             complement(24195..24305)
                     /locus_tag="OHN27_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCQ2286183.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(24322..25269)
                     /gene="hyfC"
                     /locus_tag="OHN27_001731"
     CDS             complement(24322..25269)
                     /gene="hyfC"
                     /locus_tag="OHN27_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCQ2286184.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     DSTHIDMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLTCLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25280..27297)
                     /gene="hyfB"
                     /locus_tag="OHN27_001732"
                     /pseudo
     CDS             complement(25280..27297)
                     /gene="hyfB"
                     /locus_tag="OHN27_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27297..27914)
                     /gene="hyfA"
                     /locus_tag="OHN27_001733"
     CDS             complement(27297..27914)
                     /gene="hyfA"
                     /locus_tag="OHN27_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCQ2286185.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKNGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28167..28637)
                     /gene="bcp"
                     /locus_tag="OHN27_001734"
     CDS             complement(28167..28637)
                     /gene="bcp"
                     /locus_tag="OHN27_001734"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCQ2286186.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28637..29209)
                     /locus_tag="OHN27_001735"
     CDS             complement(28637..29209)
                     /locus_tag="OHN27_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCQ2286187.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAKLVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29355..30233
                     /gene="dapA"
                     /locus_tag="OHN27_001736"
     CDS             29355..30233
                     /gene="dapA"
                     /locus_tag="OHN27_001736"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCQ2286188.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30250..31284
                     /gene="bamC"
                     /locus_tag="OHN27_001737"
     CDS             30250..31284
                     /gene="bamC"
                     /locus_tag="OHN27_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCQ2286189.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31497..32210
                     /gene="purC"
                     /locus_tag="OHN27_001738"
     CDS             31497..32210
                     /gene="purC"
                     /locus_tag="OHN27_001738"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCQ2286190.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32378..33241
                     /locus_tag="OHN27_001739"
     CDS             32378..33241
                     /locus_tag="OHN27_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCQ2286191.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33256..35271
                     /gene="tmcA"
                     /locus_tag="OHN27_001740"
     CDS             33256..35271
                     /gene="tmcA"
                     /locus_tag="OHN27_001740"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCQ2286192.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHVLKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35345..36043
                     /gene="ypfH"
                     /locus_tag="OHN27_001741"
     CDS             35345..36043
                     /gene="ypfH"
                     /locus_tag="OHN27_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCQ2286193.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMVLESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36153..36353)
                     /locus_tag="OHN27_001742"
     CDS             complement(36153..36353)
                     /locus_tag="OHN27_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCQ2286194.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36381..37508)
                     /gene="dapE"
                     /locus_tag="OHN27_001743"
     CDS             complement(36381..37508)
                     /gene="dapE"
                     /locus_tag="OHN27_001743"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCQ2286195.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLLYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37512..37868)
                     /locus_tag="OHN27_001744"
     CDS             complement(37512..37868)
                     /locus_tag="OHN27_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCQ2286196.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9227 a         9825 c         9998 g         8903 t
ORIGIN      
        1 tcaattggta ctttcatatg acacttagaa tcaatgctct ctatagtgat gaataatcat
       61 cattcgaagt caggtgggat gcctgtctga atacacctcc ttcaggatgt gggggattct
      121 gctgagcatc tatgaaactg aatgcaactt atataaaaat acgtgataaa tggtgggggc
      181 ttccgctgtt cctgccttct ttaatcttgc ccattttcgc ccacattaat actttcgcgc
      241 atatttcttc cggtgaggtt tttctctttt atctgcctct ggcactgatg atcagcatga
      301 tgatgttttt cagctgggcg gcattgccag ggatcgcctt agggattttt gtccgcaaat
      361 atgcagagct gggtttttac gaaacgctat cattaacggc taattttatt atcattatca
      421 ttctctgttg gggcggttac agggtcttta ctccccggcg taacaacgtt tcacatggtg
      481 atacccgttt aatttcccag cgtatattct ggcagattgt gtttcctgca acgctgtttc
      541 tgatactttt ccagtttgct gcatttgtag gattactggc gagcagagaa aatctggtcg
      601 gtgtcatgcc ttttaacctc gggactttaa tcaattatca ggccttactg gtgggtaatc
      661 tgatcggtgt cccgctgtgc tacttcatca ttcgggtagt gcgaaatcca ttttatttac
      721 gtagctatta ttcgcaatta aaacagcagg ttgatgccaa agtcaccaaa aaagagttcg
      781 cgctctggct actggcatta ggtgctttat tgttgctgtt atgcatgccg ttaaatgaaa
      841 aaagcacaat ttttagcacc aattatacct tgtcattatt gctgcccctg atgatgtggg
      901 gagcgatgcg ctatggttat aagctgattt cgctgctctg ggcggtcgtg ttgatgatca
      961 gcatccacag ctatcaaaat tacattccca tttatcctgg ctataccacg cagctgacca
     1021 taacctcctc cagttatctg gtattctctt ttattgtcaa ttatatggct gtactggcaa
     1081 cccgtcagag agcggtagtc agacgcattc agcggcttgc gtatgtggac ccggtggttc
     1141 atctgccaaa tgttcgcgcc ctgaatcgcg cgttgcgtga tgccccctgg tctgcgcttt
     1201 gttatttacg catccctggc atggaaatgc tggttaagaa ctatggcatc atgctgcgga
     1261 ttcaatacaa gcaaaaactt tctcactggc tgtcaccctt gctggaaccg ggtgaagatg
     1321 tttatcagct ttcgggtaac gatctcgcgc tgcgactgaa tacagaatcg caccaggagc
     1381 gcattaccgc actggatagc catctcaagc aatttcgttt cttttgggat ggcatgccga
     1441 tgcaaccgca gattggcgtc agttactgct atgtgcgctc gccagtgaat catatctacc
     1501 tgctgctggg agagctaaat acggtcgccg aactttccat cgtgaccaac gccccggaaa
     1561 atatgcagcg tcgcggggca atgtatttgc aacgcgaatt gaaagataaa gtcgcgatga
     1621 tgaatcgact acagcaggcg ctggaacaca accatttttt cctgatggcc cagccgatta
     1681 ccggtatgcg tggtgatgtt taccatgaaa ttcttctgcg catgaaaggt gagaatgatg
     1741 aactgatcag ccccgatagc ttcttgccgg tcgcgcacga atttggttta tcgtcgagta
     1801 tcgacatgtg ggtcattgag catacgctgc aatttatggc tgaaaacaga gcgaagatgc
     1861 ccgctcaccg ttttgctatt aatctgtctc caacctcggt atgtcaggct cgttttcctg
     1921 ttgaagtcag tcagttgctg gctaaatatc agattgaagc gtggcaactt atttttgaag
     1981 tcaccgaaag taatgctctg accaatgtta agcaggcgca aatcaccttg cagcatcttc
     2041 aggaattagg ctgccagatt gcgattgatg atttcggcac cggctacgcc agctatgcac
     2101 ggcttaaaaa tgtgaatgcc gatctgctta aaattgacgg cagctttatc cgcaatattg
     2161 tgtcaaatag tctggattat cagatagtgg cgtcgatttg ccacctggcg cgaatgaaga
     2221 aaatgctggt agtggcagag tacgttgaaa acgaagagat ccgcgaggca gtgctctctt
     2281 tggggatcga ttatatgcag ggttatctta ttggtaagcc gcaaccgtta attgatacgc
     2341 tgaatgaaat cgaacccatt cgcgaaagtg cctgaataat gcgggccgac atttctcgtc
     2401 ggcccgcaaa gcattaagcg gcgatttcag gtgtactttc ttcttcaatt ttcaaccgcc
     2461 agccagccac gccttcccag tattcttgct ccttttccag atcaagcagt accagcgcat
     2521 tctgactaaa ccagtcatgc gggaaacgca gtgtccagtg actgtcatca gtaatcagtg
     2581 tcaatgttgg cggtgtggtg gttgcctgac gttgattgtt tagtaatacg ccaaggcgca
     2641 atagctgtat cagtggcagg aactgtttct tcttaaacaa ggtaaagcgc ggcagatcgt
     2701 cgagcttaat cgctttacgg tgatagcgca ccagtgtcgc catcatcagc tgctgttcct
     2761 gattaaaacc cggcaagtca ctgttttgca gaatataagc ggagtggcga tgcaaaccgc
     2821 tgtggttgat attcaacccg acctcatgca gcatggcggc ccatcgcagt agcgcctcca
     2881 gttgcggatg cgccagcttc ggttgctgtt cccgccactg ttcgtacatt tgcatagtgg
     2941 tatccagcac ccgtcgggcc tgttcgctgt cgatgtgata ctggttggcg aggctgctgg
     3001 cggtgcgact acgcacatcc tgatgacgga aacgtccttc catttcatac agtacgcctt
     3061 cgcgtaacgc cccgtcagaa aggcgcagtt cacggatggc taaagcatca aacacaccgc
     3121 ataaaatcgc cagtcccgga acgaagactg ttttccgctc ttcggaaaga cccggtaaac
     3181 tcagcgatgc gaaattacgg tgacgtaaaa cttcttttac cagtttttcc agacgttccg
     3241 gggtaattat cccgtctttc tcgcccattt ccattaacac ttcatgggcg gcttttatgg
     3301 taccggaagc gcccattgca acgttccagc cctgaatacg gaattgccag gttaaagttt
     3361 ccagtttttg tgctgccgcc atgcgagcgc gctgaaaatt ctctttattg atgaccccgc
     3421 caggaaaata aagctgggca aagctgacac aacccatccg gcggctttca acgagaatag
     3481 gttcgaaatt ttcaccaatc accagttccg tagatccgcc gccaatatca ataaccagtt
     3541 tgcgaccttt ttccggttgg gtatgttcca ctcccataaa aatcagacgg gcttcttcat
     3601 taccggaaat aatttcaatc gggtagggaa tgaccttttc cgcgcgtttc agaaagtcag
     3661 tggcgttcag cgcctgacgc agcgtatggg taccaactat acagacgctg gcaggagaaa
     3721 acccttgtag ccgttcggca aaaagcgaca gacagttcaa accgcgcgtc attgcctctt
     3781 cactcaacat attatctggc cccaggccgt ccgccagatg cacccgctgt ttcaggcggc
     3841 caataatctg catggcacca tctaccacac gggctatgac catgtgaaaa ctgtttgaac
     3901 caagatcgac cgcagcaaac tcctgcggac gaggggattt atcgtgtatt ggcatagggt
     3961 tattcaggtt gttcgagtga tttgatgtag tcataaatcg ccaactgcgc ccgtactttg
     4021 cggcgattgc cgcggggaac gtagcgatta ctgagttctt tatcgatata acgagctttg
     4081 accgtatcgc tgaacaatat gtcgatgatg tccagcaccc gctgcttcag gcgcggatcg
     4141 agcagcggcg tcgccacttc aatacgataa tcaatattgc gcgtcatcca gtcggcggaa
     4201 gaaaggtaga cctttttatc gccgccattt tcaaaaatat aaacccgatc atgttcaagg
     4261 taacggtcaa caatactgat ggcacgaatg ttgtcgctaa tgccttccag attggggatc
     4321 agcgaacaca ttccgcgaac cagcagatta accggtacgc cggagctgga ggccgcatac
     4381 agacgatcaa ccaggccttt atcgacaagg ttatttagct tcagggtgat accactgggc
     4441 agcccttgct gcgcgttggc gatttcgcgg tctaccattt catataacag acggcgagag
     4501 ttttgcggtg acaccattaa ataatcaaat gtcaccgggc ggtatgggtt ttcaataaag
     4561 ttaaataccc gccgtacttc gttggtgata cgcgcatctg cggtcagcaa cgaatagtca
     4621 gtataaagac gcgcggtttt ttcgttaaag ttcccggtcc cgatgtgtgc gtaacggacc
     4681 acttcaccgt tttctttacg tgaaatcagg aacagtttgg cgtgaatttt cagccccggc
     4741 gcagagaaga taacgtgcac gcctgcttct gtcaggcgct tcgcccagtg aatgttggct
     4801 tcttcgtcga aacgcgcctg taactcaacc accacggtca ctttcttgcc gttgtgcgca
     4861 gcgtggatca tcgagtcgat gatacgtgaa tcttttgcca cacggtaaat gttgatttta
     4921 atcgccagca cgctcggatc gaacgaggcc tgacgcagca gttccagcac atgctcaaag
     4981 gtgtgataag gataatagag caacacatcg cgttcgcgaa tggcatcaaa accattgcgg
     5041 aactgggctt tatcaaacca aatgtggcgt aaacgcggca gtggtttgtt caccagattg
     5101 gctttgccga cattggggaa attaataaag tctttaaaat tatgataacg accgccgggg
     5161 acgatggagt cgtagcggga aatagtcagt ttttcgcgta acacttcaac cagcgcattg
     5221 ggcatatcgc gctgataaac aaaacgcacc ggctcagcag ttaaacgctg cttgagactg
     5281 gaagacatca actccatcag gctggcttcc atctcatgca ctaaatcgta ttcggcatcg
     5341 cgggtcatct tcattgaata ggcattcagc gcgtcataat caaagaagcc tttgaaaata
     5401 tcatcgaggc agtaacgcag aatgttatcc agaagaatca tcggtttgcg tcgacgcggc
     5461 gcttctggcg gtaaattcac aaagcgtggc actttatctg atgggatctc cagcagcgcg
     5521 taacggatgg tatcgccacg gataatttcc accgccaaat aggtgtaatc atctttcagg
     5581 aactgcacta agtcagtgtc aggattgatt aaaatcggcg taatgtgctg acgcagatac
     5641 tgcttaaaat aatgacgaag ccagttttgt tgattgacgg agagctggcg ttcattaatc
     5701 aggaagatct ggttacgcgc catctccagc agcagctcgt tgtagaggcc gtcgaattcc
     5761 tgatcggctt tcagcacccg ggactgaatt ttgcccagta aatggcggga atgagagttg
     5821 gagccttgtt cttcgctaat aatgatgcgt cgcttcagtt cagcgaagcg gactttatag
     5881 aactcatcaa ggttattgga atagatcccc aggaaacgca tcctttcaat cagcgggtta
     5941 gatttgtccg ccgcttcctg aagcacgcgt tcattgaacg ataaccaact gagctctttt
     6001 tcgatgtata gcttttcctg acccattacc acttttactc cgttttattc acgggacact
     6061 gcctggatat tatggcgagc aatattggca gatgtccaac tatgccagaa aagggggaaa
     6121 aaaactgctg gagcttgcga tttacaggtg aattgaaaaa cgccagcggc agagctggcg
     6181 ctttaactac ggggcattac tcgtcggcag cgtagccctg cggcggcaga cgttgaccat
     6241 ccagccacgc ggcgttttcg tgcattttca gacgaccatc ggcaaaccag ctaatcacca
     6301 gtggataaat agcgtgttcc tgggtttgca cgcgggcggt gatgtcatct tccgtatcac
     6361 cagcaaatac cgggactttc gcctgtaaaa taaccgggcc accgtccagt tcatcggtga
     6421 cgaaatgcac cgatgtaccg tgctcttcat cgccattttc cagcgcctga cgatgggtgt
     6481 gtaatccggg atatttcggc agcagagaag ggtgaatgtt cagcaaacgc ccggcatagt
     6541 gggagacaaa cgccgggctg agaatgcgca taaaaccagc cagcacgacc acatcgggtg
     6601 cgtacatgtc gatttcatga atcaactccc ggtcataggc ttcacgactg tcaaacgcgc
     6661 tggcgatgag cgtatgcgtt gcaatacccg cctggcgggc gcgttcaagg ccgaacgcgt
     6721 cggccttatt gctgaaaact gcccgtacgg tgcctttaat tttgttggtt ttacaggcgt
     6781 caataattgc ctgtaaatta cttccgttgc cggaaataag caccacaata ttcattattc
     6841 gataaccacg cgttgttcgg aatcagaggc tttgatgata ccgattttcc acgcgttttc
     6901 accgttggca ttgagcaggg cgagggcttt gtccacttcc ggagcaggca gggcaataat
     6961 catcccgacg ccgcagttga aggtgcgata catttcatgg cgttcaacgt tacctgccgt
     7021 ttgcagccag ttgaacactt ccggccactg ccaggaagat tcatcaatca ctgcctgggt
     7081 attatctggc aatacgcgtg gaatgttttc ccagaagccg ccgccggtca ggtgcgcaat
     7141 ggcatgcaca tcgacctttt caatcaactc cagcactgac ttcacgtaaa tgcgggtcgg
     7201 tgccagcaga tgatcggcta atggcttacc atcaagttcg gtggtttgcg gatcacaacc
     7261 gctgacttca agaattttgc gcaccagcga atagccgttc gagtgcggac cgctggaacc
     7321 gagtgcaatc agcacatcgc cgtcgctgac tttagagccg tcgatgattt ctgatttttc
     7381 taccacgcca acgcagaaac ccgcgacatc gtaatcctca ccgtgataca tccccggcat
     7441 ttctgccgtt tcgccaccca ccagtgaaca gcccgattgc agacatcctt ccgcgatacc
     7501 gctgatcacc gctgaagcgg tatcaacatc cagttttccg gttgcgtaat agtcgaggaa
     7561 aaacagcggc tctgcacctt gcaccaccag gtcattaacg cacatggcga ccagatcaat
     7621 accaatggtg tcgtgacgtt ttaagtccat tgccagacgc agcttggtac ctacgccgtc
     7681 agtgccggaa accagcacgg gttcacgata tttttgcggc aatgcacaca gcgcaccgaa
     7741 gccgcccaga ccgcccatca cttccggacg acgcgttttc ttcactacgc ctttgattct
     7801 tccaaccaga gcattacccg cgtcaatatc aacaccggca tctttgtagc taagagaggt
     7861 tttatcggtc actgcttggg tccccacgcg ttacttgcgg tagaaaaata aaattcggcg
     7921 caattctaac agggaaagca aacgtttgcg agactgcttt acacaacctt tttgcacgtc
     7981 ttttgcccag gcgcgcggcg aaagaagact tgtaccaggg taaaggttag ttttcggatg
     8041 gaataatctt ctttcataac catttgaata taaaataact ttatctcaaa ccgttatcat
     8101 tttgactaaa gtcaacgaaa agaatattgc cgccttgaag aaaggaggta taatccgtcg
     8161 attttttttg tggctgcccc tcaaaggaga aagagtatga agatcgtgga agtcaaacac
     8221 ccactcgtca aacacaagct gggactgatg cgtgagcaag atatcagcac caagcgcttt
     8281 cgcgaactcg cttccgaagt gggtagcctg ctgacttacg aagcgaccgc cgacctcgaa
     8341 acggaaaaag taactatcga aggctggaac ggcccggtag aaatcgacca gatcaaaggt
     8401 aagaaaatta ccgttgtgcc aattctgcgt gcgggtcttg gtatgatgga cggtgtgctg
     8461 gaaaacgttc cgagcgcgcg catcagcgtt gtcggtatgt accgtaatga agaaacgctg
     8521 gagccggtac cgtacttcca gaaactggtt tctaacatcg atgagcgtat ggcgctgatc
     8581 gttgacccaa tgctggcaac cggtggttcc gttatcgcga ccatcgacct gctgaaaaaa
     8641 gcgggctgca gcagcatcaa agttctggtg ctggtcgctg cgccagaagg tatcgctgcg
     8701 ctggaaaaag cgcacccgga cgtcgaactg tataccgcat cgattgatca gggactgaac
     8761 gagcacggat acattattcc gggcctcggc gatgccggtg acaaaatctt tggtacgaaa
     8821 taaagaataa aaataattaa agccgacttt aagagtcggc ttttttttga gtaaagcgcc
     8881 tataacacat aatacagagg ataatactat gacgcgccgt gctatcgggg tgagtgaaag
     8941 accgccactt ttacagacaa tcccgcttag tttgcaacat ttgttcgcca tgtttggtgc
     9001 aaccgtcctg gtgcccgtct tatttcatat taacccggcg actgtactgt tatttaacgg
     9061 tattggaacg ctgctgtatc tcttcatctg taaagggaaa attccggctt atcttggttc
     9121 cagctttgcc tttatttcac cggtattgtt actgttgccg ttagggtatg aagtcgcgct
     9181 gggcggcttt attatgtgcg gcgtgctgtt ctgcctggtt tcttttatcg tgaagaaagc
     9241 ggggaccggc tggctggacg tgctgtttcc acctgcggca atgggcgcaa tcgttgccgt
     9301 catcggtctg gagctggcgg gcgtagctgc cggtatggcg ggtttactcc cggctgaagg
     9361 gcaaacgcca gactccaaaa ccatcatcat ctctattacc accctggcgg tcacggtttt
     9421 aggttccgtg ctgttccgtg gtttcctggc aattatcccg attttaattg gcgtgctggt
     9481 ggggtacgcg ctctctttcg caatgggaat tgtcgatacc acgccgatta ttaatgctca
     9541 ctggtttgcg ctgccaaccc tctatacgcc gcgcttcgag tggtttgcca ttctgactat
     9601 tctgccagcg gcgttagtgg ttattgccga acacgtaggg cacctggtag taacggctaa
     9661 tatcgtcaaa aaagatctgc tgcgcgatcc aggtctgcac cgttcgatgt ttgctaacgg
     9721 cctgtcgact gtgatctccg gcttctttgg ctctacgcca aataccactt acggagaaaa
     9781 cattggcgtg atggcgatca cccgtgttta cagtacttgg gttatcggcg gggcggcgat
     9841 tttcgctatc ctgctttcct gcgtcggtaa actggctgcc gctatccaga tgatcccatt
     9901 gccggtgatg ggcggcgttt cgctgctgct ttatggtgtc atcggtgctt ccggtattcg
     9961 tgttttgatc gaatcgaaag tggattacaa caaagcacag aacctgatcc tgacttccgt
    10021 gattttgatc atcggcgtca gtggcgcgaa ggtaaacatc ggcgcggcgg agttgaaagg
    10081 tatggcgctg gcgaccatcg tcggtatcgg cttaagtttg atcttcaaac tgatcagtgt
    10141 gctgcgccct gaagaagtgg tgctggacgc agaagacgcg gatataacag acaaataatg
    10201 taccgaccgg gcagtgttgc tgcccggttc aaacatcatg ggattctgtg gtaaacttct
    10261 cgcgattttg tgaaatcctg gttgaggtat ctctgaacac accggcacag ctctctttgc
    10321 cactttatct tcctgacgac gaaacctttg caagtttctg gccgggggat aactcctctt
    10381 tactggctgc gctgcaaaac gtgctgcgtc aggaacatag cggttacatc tatctctggg
    10441 cacgcgaagg cgcggggcgc agccatctgc tgcacgcggc ttgcgcggaa ttgtcgcagc
    10501 gtggcgatgc ggtgggctat gtcccgctgg ataaacgcac ctggtttgtt ccggaagtgc
    10561 tcgacggtat ggagcatttg tcgctggtct gtatcgacaa cattgagtgt attgcaggcg
    10621 atgagttgtg ggagatggcg attttcgatc tctacaatcg aattctggaa tcgggcaaaa
    10681 cacgattgtt gatcaccggc gatcgtccac cgcggcagtt gaatctggga ttaccggatc
    10741 tcgcgtcgcg actcgactgg gggcagatct acaaattgca gccactttct gatgaagata
    10801 agttgcaggc gctacagtta cgcgcgcgtt tgcgtggttt tgaactgccg gaagatgtgg
    10861 ggcgtttctt gctgaagcgg ctcgacagag aaatgcgcac gctatttatg acgttggatc
    10921 agttggatcg tgcgtcgatt accgcgcaac gtaagctgac cattccgttt gtgaaagaaa
    10981 ttctgaagtt gtagataagg tgtttattgt cggatgcggc gcgaacgcct tatccgaact
    11041 acgcgaagtt aatccatagt attcgtaggc cggataagac gtggcaagcg tcgcatccgg
    11101 caatgtgctt caactagcga tggagcacat cttaacccac gatttccagt acctgctccg
    11161 gtggacgacc aatccgcgct ttgccattcg cgaccacaat cgggcgctcc attagcttcg
    11221 gattatccac cattgcctga atcagcgcct cttcgctaag cgaactgtca gccaggttca
    11281 gttctttata aagatcctct ttctggcgca tcaattcacg ggcgctattc atccccagca
    11341 ttttcagcaa atcgcgcagc gttgccgcat cggcgggtgt ctcaaggtag agtaccactt
    11401 ctggctccac gccgttttct ttcagcaggt tcagcgtttc ccggctcttt gaacagcgtg
    11461 ggttatggta gatttttacc tgtttggtca ttgttcttcc tttaatgcga attacatctt
    11521 ggtataaggc ttaaagcgtt cctgcagctg gcgcaactgg tcgatgcgcg catcgtaacg
    11581 cgcttgttgc aggctgccta atttcacctg cgaactggcg ctactcaaca gcgaaatggc
    11641 ctgatcgagt cgtccggcga gcgcataacc ttctgctcgc gcagccagct cctgatcgcg
    11701 gttatttagc gcggcttccg cctgtgccag taaatcccag ccgttgctgt catctttatt
    11761 attaaaggtg tagcgattga gaatattggc cgcttcttgt ggttgaccgc cttgcagata
    11821 agcgtttgcc aggttgagct gcaacaccgg attggtgcgc aaatcacggg catttttcag
    11881 gcgattgatt gcctcattgg ctttgttttg cccaagatcg atatcggtag ccagatcgag
    11941 ataccatgcg ttgccaggtt ctgccgccag taacggttgc agcgttttac gcgcctcgtc
    12001 gtatttattg gcttccatcg cctgtaaagc acgaccatat tgcgccgctc gttgctgacg
    12061 aacgtttcct ttcgcccatt catccagcaa atcactggtg agctggttac gtccggaatt
    12121 atacatcccc agtgtgcgcg ctttcgccag atagaaatct tccgacgact gcaccaccat
    12181 cggtcgcatc tgattagcac ggttgcgggc atctgccaga cgactttccg gcaacgggtg
    12241 agtcaataaa atttccggcg ggcgcgagga gtaacgcgcc tgatcgagta atttttcgag
    12301 gaaggttggc atcgcctgcg gatcgaatcc cgagcgttgc agcacctgaa taccaatgcg
    12361 gtccgcttcc tgttcatttt gctgggtgaa actgatcatc ccctgacgtg ttcccgccag
    12421 tgtaccggtc agcgccgcca tccccgcctg cggactggcc atcgccagta aaatagaacc
    12481 taacgcgccg acccaggtca gcggcgcgct gcgttgttga tcttccatcg ctcgcgccag
    12541 gtgacgttgg gtgacgtggg agatttcgtg cgccataact gaagccagtt gactttcgtt
    12601 atcggaataa cggaacaggg cagagtgcag caccacgttg ccgccaaaga aagcaaaggc
    12661 gttaatttcg tcgttgttga tcagaaaaaa atgaaacggt gtcttaaccg aattggcatg
    12721 cgaaaccaga cgcatcccca gcgaattaat atattgcgtt aacagcgggt cattaattaa
    12781 cggcgcgctg ccgcgtagct ggcggacata atagtcgccc atctgcattt cctgaccaat
    12841 ggaaagcgtg cttcctgcgg aggttcccat atccggcaag gtgtctgcgc tgtcggcaaa
    12901 cgccggggct acctgaccaa tggtcatagc agcaatgagg gttgcaacca ggtttttttt
    12961 caactgcctg aacataacct ctatcctgta tttctgaaga tagtcatttg accgatactg
    13021 tgaaatatcg ttcccaccgc gccgcatttg agtgtaacgg cgaagattag cccactcaaa
    13081 tgacttttca gtttcctgaa tccgtcataa aaagcgaagt cttttttgtg acatttcgat
    13141 acaattgcgg atcgcaatcc cgctcttgag gttcagggaa ggtctttatg ctcgaaatgt
    13201 tgatgcaatg gtatcgccgc cgttttagcg acccggaagc gattgccttg ctggttattt
    13261 tagttgccgg atttggcatt atctttttct ttagtggcct gcttgctccg ttgctggtgg
    13321 ctattgtgct ggcctatttg ctggaatggc caactgtgcg cctgcaatct attggctgct
    13381 cccgccgctg ggcgacgtcg attgtattgg tggtttttgt cggtatattg ctactgatgg
    13441 cgttcgtggt actgcctatc gcctggcaac agggcatcta cttaattcgc gatatgccgg
    13501 ggatgctcaa taagctttct gactttgccg ccacgttgcc gcgccgctat ccggcgttaa
    13561 tggatgcggg cattattgat gcaatggccg aaaatatgcg cagtcggatg ctgaccatgg
    13621 gcgattcggt ggtgaaaatt tccctcgcct cgctggtcgg tttgctgacc atagccgtct
    13681 atctggtgct ggtgccattg atggtcttct tcctgctgaa agacaaagag cagatgctga
    13741 acgccgttcg tcgggtgctg ccgcgcaacc gtggactggc aggacaggtg tggaaggaga
    13801 tgaatcaaca aatcaccaac tatatccgcg gcaaagtgct ggagatgatc gtggtgggga
    13861 tcgccacctg gctggggttc ttgctctttg ggctgaacta ttcgctgctg ctggcggtgc
    13921 tggtcggctt ctcggtactt attccgtaca ttggcgcatt tgtggtgacc attccggtgg
    13981 ttggcgtggc gctattccag tttggtgcag gcacggaatt ctggagctgc ttcgcagtgt
    14041 atctgattat tcaggcgctg gacggcaacc tgttagtacc ggtgttgttc tccgaagcgg
    14101 ttaacctgca tccgctggtg attattttat cggtggtgat cttcggtggt ttgtggggat
    14161 tctggggcgt attcttcgcc attccattgg cgaccctgat caaagccgtg atccacgcct
    14221 ggcccgatgg gcaaatagcg caagaataat cttcagcccg acgaactgtt tcgccgggct
    14281 gatttttcag gattcctgac gtaaatagat agcccgataa cacatactca ccagcactgc
    14341 accgccgata atattaccca gcatcaccgg gagcagattg gcggtaataa aatggcttac
    14401 cgtcagtgcc ggaaaattgt ctgcgctact gtgcgccagt tgccagaagg ggggaggggc
    14461 gaaatggcga atggcaatgg cgaacggaat cacaaacaaa ttggcgatac agtgctcaaa
    14521 gccactggcg acaaacaggg tgatgggcaa aatcatggcg acgattttgt cgcataacga
    14581 acgcccgcaa taactcatcc aaagcgccag gcaaaccatc agattgcaca taatgccgag
    14641 gctgacagat tcagtaaatg tatgatgcat tttgccctcg gcgcagtgta aaaccgcgac
    14701 tccccacatc gcgttttcac tcatcaccag cccggaaaac cagattaaca aactgaaaca
    14761 ggcaatacct gccagattac cgcaggccac cagaagtgcg ttaatcagcc aagttcgcca
    14821 actaataacg ccccgacttt ttgccatcac cgtcattacc gacgaggtga acaggctggt
    14881 gccgcaaacc gccagcaaaa taaagccgag tgtaaagcaa aggccgccca ccagatgggt
    14941 taatgcctgt gaagacgggg catcggcgat aacggaaagg taaaaagtaa atccgatcgc
    15001 cataaacacg ccagcggaca tcgccattaa aaagaccgac actttactgc gggcaatttt
    15061 atgggcggca atccgttcag cgattgccgc ttttctggcg ctcaactgca agtcgaaaga
    15121 gagtttgttt ctcatgcata ctcctgcaaa agcagattac aacacctcgc gaaccgagat
    15181 ccccagacgc tgcattcgtg acagcagcgt ggtgcgcttc atccctaatc gtgtcgccgc
    15241 gccacggggc ccggcaacaa tgccattggt ttctcgcaat acctgaacaa tgcgctgacg
    15301 ttcttcttcg tcattctctg gcgtcgtcgg gtgcagcaac tgcgccattg aactgcgaag
    15361 cgctgaatct tcatttagcg tcggcagtaa acggctttgt cggacattta gatgtaaatt
    15421 cagactgtta ccacgagtca acagtaccgc ccgctcaatc acgttttcca gctcgcgcac
    15481 gttgcccggc caatcccacg acatcaactg gcgtagtgcc tcggtcggga tggcgtcaat
    15541 tgcgcgattc atatggcgcg ccattttttg cgtgaaatgc tttgctaaaa gagggatatc
    15601 ttccggacgg tcgcgcagcg gcggcaattc cagtgggaag acattcaggc gataaaagag
    15661 atcgctgcga aactggcgat cttcaaccat ttgccacaaa tcacggttgg tggcggcaat
    15721 gactctgaca tttaccggga tcgttctact cccgccgaga cgctcaatct cccgttcctg
    15781 caatacgcgc agcagtttag gctgaagttc taacggcaga tcgccaattt catcgagaaa
    15841 caacgtgccg ccatcggcaa tttcaaaacg accacgatgg gtattaatcg caccagtaaa
    15901 cgcccctttg tcatgaccga ataactcact ttccagaaga ctggcgggga tggcagcgca
    15961 gttgattttg accagcggct tgtcgcgtcg cgggctaagt tgatggatcg ctctggcgat
    16021 cacctctttg ccggttccgg tttcaccgca aatcaacacc gtactgtcgc tcttcgccac
    16081 aatatctacc tgctggagta ggtcttccat tgcctggctt tgatagataa tgtcaccgat
    16141 gcccagattc gaaaggagct gctcgctaag ctggtggttt tcttgctgca aactttcctg
    16201 caaatcggtc atgctacgcc aggcatcggc attgccaacg gcaatagcga tgcgatcggc
    16261 tatgtgttgt agtagctggc agttttcctc actaaagaga gtggaagagg tatgcgccag
    16321 caacaatgca cccggtgtat ggttgccaaa ggtaagcggt ataaggagcg tagattcgca
    16381 gccgttagat gcaagtaaga gtaataacgg gtcgcgttgc cagagaaaca gatcgtctgc
    16441 acggtgcgtc aaggtcggtt gtcgggtttg tagcgtttgt gtcagcaata cactgtcgcc
    16501 aggcatattg cgtggcagac acgcacaatg tgaggcagaa agatcgctgc accacaggct
    16561 aaacttctcg ttctttcgat gatcgcccag taccatactg actgaagcca gaccgaaaaa
    16621 atgatggatc tcacgagcga cgtcagcgat cagatcgtcg agatcaagat gtgacagcac
    16681 tgcattagta atatcaacca gtacccgatg atgatcgcga tctttctcca gcaaacgcag
    16741 cgccacctgc tgttcttcat cattgagcca tgattgcaga accggggtga ttatccgcgc
    16801 cagccagtta aacagtgtgc aatcctgggg agtaaaacga ctgccgtggg tgcgaataaa
    16861 gcgtagctcc gccattccac tactgccgag aaggacggcg tgacgcgaat aatcgaccat
    16921 ctcgccctga tactctgttg ttgccgtcac atcgctgcca tcatcatggt agcggtaata
    16981 gaagttgctt ttgagaacat gcagttcaat aagttgtacc gggctgaacg gcggcggcag
    17041 cgggatgaag gcgcgtaata aagacgcctt accgtgtttc tgcgctaacc gctccgcgag
    17101 cattccgttt accgcttcaa ttgttattcc ttgcggcgga gcaaacatcg cctcgtctga
    17161 catagccatc aacagtcctt attaatgaga aatccttctc acgtaatttt tctggccatc
    17221 agatagatgg ccggttcctg cacaatttca tccaacagat gaatgaattg aatgctacgc
    17281 gcctgccaca cagacgtcgc gtcatccgcc agtggcgcga atgcccgggt caacagggcg
    17341 gtgtggctgg cgtcaataac aatctcacca aaagtcatca cccccagcat cttacgtcgg
    17401 gcttgctcct cttcgaccag tgcaagccaa tcttcgtatt ccgtcagtgg gcaggggaag
    17461 gcgacattca gacaatcaat cacgccaacg tgatgaccga tagccagcga gtaatacatt
    17521 acctgagagc tgtgttccgg catctcgtcg ctactggcga caaacttttt tcgcagcgtc
    17581 cagaaaacaa tttccccgca ctcttcagtc atgtaacccc cggagtacga gaaagagttg
    17641 ctgaacgata ctattgagac gtggatcgtc ggcatcgcgc aaccaggtgt taacccgatt
    17701 tcctgtagga tcgtcgctta aatggcgcag gagccgatcg caaatttctc gcccctgacg
    17761 atagcccgcc agccgccgcg cttctcgctc aatggcgata cgctgtgatg gcgggatctg
    17821 cggccacaac ggttgcatag tcaccccggt gggatcgcga taatccaccg cgtgaatctt
    17881 ctgttgcagc aaaccgagcg ccacggcgaa accgtgaatg gtggcggccg gtgttggcgg
    17941 gcagccgggg atccaaacat caatggggac aatggtgtcg ctaccgcccc agacgctgta
    18001 gagatcgtgg aaaataccgc cgccgacacc gcacgcgccg taggaaacac aaattttatg
    18061 atcgggggca gactcatacg cccgaagtgc aggcatacgc atcgcccggg tgactgcgcc
    18121 agtaaataac aaaatatcgg cgtgacgcgg tgatgaaaca accttaatgc caaaacgttc
    18181 tgcgtcgaat acgggtgtga tggcggcaaa aatttcgatt tcacaggcgt tgcagccgcc
    18241 gcaatcgacg cgataaacgt aagccgagcg acggatatcc tgtagcaaat gccgcttcat
    18301 cttttgcgtt tgctcatcca gcgtgatggg ctggctgaca tgttgtgtaa gcactggact
    18361 catagctgct ccttagccac cagcggtaca tctgtatcgt cgttgatcag cgtcgcgcgt
    18421 tgtttgcact ccgggcagac gctcgcttgc gcccgcaaca tttcgcggtt ttgtggcgca
    18481 ttttgttgct gtgccaacaa ttcagcagcc agtgcgacgg ttttttgcgg ggcaaacggg
    18541 cgttcgcaac ggctgcaacg ttgtagatgg aacgtcgcgc gggtatagag atcggcttta
    18601 ttggtgacgg tcagttcaaa gttattggta agctggatgg ctctggtcgg gcacacttct
    18661 tcacaacgtc cgcagtaaat acaacgcccc agatagagct gccaggtgcg cgaattttgc
    18721 tggtcgtcgg tctggatagt cagcgcattt gccggacaag cacaggcgca ggcaccgcag
    18781 gcaatacatt ggctgggcat caggtccggt ttcccgcgaa agcctgggct gacctccagt
    18841 ggcgcgaagg gatatttcac cgtcgcggtt ccggcgcgca taatagtttt cagtaacttc
    18901 agcatcttct gtccttactt cagcggcgaa cggttacgat caatgccgta gcgttcgatc
    18961 tctttatacg gcacggtttt tgactggcgc ttgcgcacat ctaccagcgt cacacggtcg
    19021 gtacaggagt agcagggatc aaggctaccg ataatcagcg gtgcgtcaga aacggtattg
    19081 ccgcgtagca tgtaacgcag cactggccag ttggcgtagg tggcggcacg acagcgccag
    19141 cggaacaatt tttggttatc accgagcatg ctccagtgca cgtcttcacc gcgtggcgct
    19201 tcaacaaagc ccagcgcgaa tgcgtgaggt ttatagctaa agccttcggt cagcagcggg
    19261 gtatccggca tgttgtcgag ggcaaattcc agcattgcca gcgaatcaaa cgtctctttg
    19321 acacggacca tcacgcggga gaaaacatcg ccgccggtga aggtaaacag tgtttttgga
    19381 atattgccgt agtcggcgta ggggtgatca aagcgcaaat cacgggcaaa accactgccg
    19441 cggatcagcg gccctacagg gctataatca cgggcgattt gtcggtcgag aatgccaatg
    19501 ccctgagtgc gttgttccat attcggcgta gcaagcagca tctctaccag ctccgacacg
    19561 tcggcgcgca tctcgcgcac cagtttcagc gtttgcagac gttgctcttt gagaatatcg
    19621 cggcgaacac caccaatcag attcagaccg taggttttac gcgacccggt cagcaattcc
    19681 gccatcgtca tcgacttttc ccgcacgcgg aaaaattgca taaagccggt atcaaaacca
    19741 acaaaatggc aggagaggcc aaggttgagc aaatgactgt gtagccgttc gacttccagc
    19801 agaatcgagc gaatggtatg cgctcgttgc ggcacctcaa tccccagtgc attttcaacc
    19861 gagttggtat aggccacact gtgggcaaaa ccgcaaatcc cacacacgcg gtctgataag
    19921 aaggtcactt cgttgtagcc catccgcgtt tctgccagtt tctccatgcc gcgatggaca
    19981 taaaacaggc ggtaatcagc atcgacaatt tgctcgccat ccacaaacaa gcggaagtga
    20041 cccggttcat cggaggtgat atgcagcggg ccgacaggga tcacctgcgc atcgctgttg
    20101 ccctcattga taaacggata cgtttcggaa tcagtcgtcg gttcagggcg cagtcgataa
    20161 tccatcgcat ctttgcgcag cggatgcata tcttccggcc agtcatcggg caacaccagg
    20221 cgacgctgat ccggcaggcc aaccggaatc agcccgtaca tatcgcgaat ttctcgctcg
    20281 ccccagaccg cggcagggac gcgcggtgtg actgacggaa actcccgact gtcggcatcg
    20341 accagtgcct tcactacgat ccagcatttt tcggcccctt ccatagaaag ggcataataa
    20401 accgcgtaat gaccgttaag tgtccgctcg tcgttgccaa acaggaccgg aagccagcca
    20461 tcatgttgat aataaagata atgtacaacg tcaggcagca gattgatttt caccgtaatg
    20521 gtgacctgtt caggcgtttg tcgctcttca tccagcaccg cgccggggaa ctgcgttttc
    20581 agggcggcga gaatcgcttc gccacgattt gacgatgaat taacgttcac tgtgtttctc
    20641 cgaaacagat gcggtgcctg aaggcaaaaa atcatgccag gtgctacgtt gtgcaggcag
    20701 gtcgtgcgtc cctgagagga ctatagtgga agcgcccgcc aggatcctga tgacaggttg
    20761 tggaatatgc gttcccatcg ccagcatcat gaccagcaga atcaccattg gcgaggtggt
    20821 caaccagccg agatcacccc ggttaacggc ctgcggcggt ttcgccatta acacccgcgc
    20881 tgccatccgt accaggcccg ccagcaccag cgttaacagc aataacagca ggacgataat
    20941 cagcaggtga ttacgtgcca gtccggcggt aatggtcata aattcgctaa gaaaaatgtt
    21001 gaagggcggc atccctgcca gcgccagcgc accgccgcca aacagcacgg cggtaaatgg
    21061 catgattttg agcatcccac agacgacgtt gagatcgcgc gtgccgtact tgagcagtac
    21121 attgccggaa ccgcagaaca gcagcgtttt tgccagactg tggtttaaga tgtgcagcag
    21181 cgcggcaaaa attcccagcg gcccgccaat gcctagcgcc accgcgacca gccccatgtt
    21241 ctccacgctg gagtacgcca gcagacgctt aatgtcccgc tgtaccagaa tgaaaaatgc
    21301 cgccacggca accgacaaca tgccgaagat gagcaacaac cggttgggga aatcgctgcc
    21361 gatggcttgg caaataatga tgtaatagcg aatcagcacc aacagcgcgc agttcagcaa
    21421 tacggcggag agcagggcgc tgaccggact cggcgcttca ctgtgagcat ccggcagcca
    21481 ggcgtgcatg ggaaatagcc cggttttggt accaaagcca attagcaaaa acacaaaggc
    21541 caacagcatt aatgttgggt caagcaagga cgattgctta agaacctcgc tccagaatat
    21601 cgccatttct gcctgcggca taacgctggc ggcgttggcg tataccagca cggtaccgaa
    21661 cagaccaaaa gcgacaccaa cagtacaaat aatgatgtac ttccatgcag cttccagcga
    21721 tgaacgctga ccgtaaatgc ctaccagaaa cgccgagctt aaggtggtgg cttcgatcgc
    21781 cgcccacatc acaatcaggt tattgctggt aacaaccagc agcatggtga acaaaaacag
    21841 atggaagaaa ccgtagtaat cgcacagcgt aacgggtgaa agctcgccgt gtgccacttc
    21901 atgacgcatg tagccaatcg agtaaatacc ggtgagaaag ccaatcacac caagaatggc
    21961 gaggaacaaa ccgcccagac catcaatatg aagccacagt cccgcagcga atatttctcc
    22021 tgcatcagcg gcagtttgga ccacccagag tgccagaatc agcagcagtg tgatccctaa
    22081 gctatgtaat acggtcaccg tgcgagtcgc agaaagtctc cgtttccggc aggcaaaaca
    22141 gagcagcgaa aaaagcagcg gcgtgagcag gagtaaagcg aacatcacag aataactcat
    22201 ctcattatcc cttcagcgcg gtcaagttgt tcacgtccag cgtgccgtgg gtacgccata
    22261 tttttcttgc cagtaacacc atcacaatga cggcgaagat ggcgtcggta gcgataccta
    22321 tttccaccag ttccggtgct cgccaggcaa gaagcgccag caccagatgg gagccgtttt
    22381 ccatcaggca gtaaccaaaa atttgccgca ggatattgcg ctggctgaca atgcacagca
    22441 ggccaagcag aaaatgacct aacgctaccg ccagcgccgg tttcagcccg gtagccatcg
    22501 gtagcttcac gggctgaacg acaaatgcgc aaagcaggac aattaacgcc gcgagcagtg
    22561 ccatcattgc cggaccgaat aacgcttttt ccgggatgtt ctggggaata tttcgtgcag
    22621 cgtaagtcat gattaacggt accagcagca ctttggtgat aaaggcgctg gcggaccaga
    22681 tcagcagttg ctctgcggcg aacaggcacg agagggtggc gaaaatagac accagcacca
    22741 gtgactggca ggcgtaaaat ccgcatgaca ggcgatagct tttgacgcta atcacaaaca
    22801 gtgatgtcag catcatcagt cccgccagat tatttacgat catagaaccg gtcattcatc
    22861 atctccttac tgcaaccagg tcgcggcgat tacgctggaa atcagcgaca tcacaatcag
    22921 tacaatcaac accaggcgca ttgaccctgg cagcggtgcg gcatcggcga cggcctcgct
    22981 cggtttgcca ggcacgacgc gaccaaacca gcgaataaac caggcgaaac tggcgaccga
    23041 ttcaatcatc agcagaatca tggcgggcag caggatccag tactccactg acaacgcaaa
    23101 accggcagca aacagcggga atttactgaa gaagccgttg aacggcggca cgccggtaat
    23161 tgccagcgct gccacgcaga aacccacgcc aggcaacggc agggtgtgca atacgccacg
    23221 cagacgcggc aacaagcgcg tgccgcagct gtaactcagc gcacctgcta caaggaaaaa
    23281 caggctttta gcgaacgcgt ggttgacgat gtaggcgatg ctaccctcca gcgccagccg
    23341 cgagccgaag atggagagcg acaagccgaa gaacatccag ccaagttgag tgatggtcga
    23401 ccaggccagc aaccgcttca tatcctgctg tggcaaatac atcagaaatc cataaagaat
    23461 ggtgaccagg gccatgacca tgccaacgcc gccaatcaca tgcgggatat tgccgccgtc
    23521 gataatggcg cgggcaaaaa tgtaaacgcc cactttcacc atcgatgcgg cgtggagata
    23581 ggcgctgatc ggtgttggcg cttccattgc gtccggtagc cacgcttgca tcggtagctg
    23641 ggccgatttc ccccacgcgg caaacaggat gccgccataa accagataac gtgcgtcgcc
    23701 gtgtaactcg ctcatcgcgc taagcgcaaa cgttccggtc tgcaaaaaca gcgtggcggc
    23761 ggcaagatac aaccccaacg agccgatatg agtgataagt aacgctttta gtgctgaacg
    23821 ctgcgcttta tcgctctggt aataactgat caacgcccag gagcagccgc ccgtaatttc
    23881 aaaaaacaac aactgaccga gcagcgtcga ggagagtacc agtcccgcca tcgcgccgat
    23941 aaacaccagc aaaaatgcgt aataacgatt cgtgccgtta tgcgggtgtt cgcgattttt
    24001 atccgtcaga taacccgtgg agtagatcgt gaccagcaaa ccgagaaaca ccaccacaaa
    24061 cagaatcagc gtactcacgc gatcaatgac caggccaaac aatgccacat cccccacgtt
    24121 gaccaacgtc aacgtgacgg caactttatc ggcctgataa aacgcggaga tcagcgacaa
    24181 catgcacagc gtggtcagtg cggcgaacaa aacccccatt cggcggcccg acgttgtggc
    24241 gaaaacgaaa cgaccagtgc gccgataaaa ggcagcatta acgtcgtcag agcaagattt
    24301 tccatattcc gtcagtgctc cttacagacc ggttaaccag aagacccatg caagcgcagc
    24361 aaggctgaag ccaagccagg tcacatggtg aatgagtaaa aaacgcccgc gtgccagcgt
    24421 gttttctgcg attgaagcca gtacaaaaat cagcaaaacc ttgagcagcg taacgacaag
    24481 tgaagtcagc aggcaggtga gagaaagttc ttgcgcgcgc ccaaagggca gaaacagggc
    24541 cacaaacagt gctgccatca cgacctgttt cagccccagc ccccatttcg ccaacgccag
    24601 cccggcaccg gaatattcgg tcagcgggcc ttcctgtaat tcctgttctg cttcagcaac
    24661 atcaaaggga atttttccca tctctatgaa gcaggcaaaa ccacaggcca gtaacgccag
    24721 tacggtggtt agcggcgagt tccagcccat cgccagcgta ttgctgatca tgtcgatatg
    24781 cgtggaatcc gctatcagcg ccaataccag cagtgagaga atgagcatgg gttcgaccag
    24841 aatgccgagc gtcaactcgc gactggcacc gactcccgca aacgggcttc cggtatccag
    24901 ccctgaaaga gcaaagaaaa aacgaaacag ggcaagaaga tagataaggg tgatcagatc
    24961 gccgccgccc gcaaaagggg aaacggtaat aaatagtggt agggccatcg ccagcaccag
    25021 catgctgctg attaataccc acggcatcag gcggaacatc agacctgaag atgtcggcgc
    25081 aacttcctgg cgtttaaaca gtttgtggat atcgcgataa tcctgccaga tccccggtcc
    25141 gcggcgggag tgcattcgcg cgcgtatctg ccgggaaata cccgtaaaaa gtggggttag
    25201 catcagcaga atcacggcct gtgctaacgc aaaaatgacc agatatccgt cgcaaagagt
    25261 ttgtctcatg gtgatttcct tagacggcaa tagcgattag cagcacaacc agtgcggcaa
    25321 ccacatacag gcaatagaga cgaaagtcgc cgctttgcag atgctgtatt tctttggcca
    25381 gccgttgggt ggcgctcacg atggggcgga tcacccgctc atcccagaag ggttctgtgc
    25441 tctgagccct ggcggtgacg tgcgcaagac ctttatttag cctcagcgta gggtcgagct
    25501 gttgtcgtag acgaaatagc gcagaaaaga ccacacgcag cggctgcatc acgccattgc
    25561 ctgacggggc catcgcattt tcccagccgt agccgcatgc ccaggcatct cctgtgcgac
    25621 gaaacgctgc gcggcgcgaa cgacaccaca gccagtaaag gccaggcagc aaaggcatcg
    25681 ccagtagtaa cagcaacagt aatgagggag tgacccgtgt atgaaacgtg ccgggtacaa
    25741 gtgctattcc gctggcgaca gtgacgggag gggtatcggt aaaggcatgg gcaatatgca
    25801 ttatcttcgg tgccagccaa ctggcactaa gcgcaattaa tacgcagagt gccgcgagca
    25861 gtagcatggc gacgatcatc gtatttggca cttcctgtgc ctcttcagcg tgtgtactgc
    25921 gcggcgcacc acagaaagta ataccgtaca ttttgacgaa gcacattact gccagcccac
    25981 cagtgactgc cagcatcacc atagcaatag gacccgcaag ttgtagcgct acggcttcca
    26041 cacgacttag tgagaacagc gactgccagg tgtaccattc gctgataaaa ccattcagcg
    26101 gaggaagggc tgatatcgcg aggcaaccaa tcaggcatgc tgcggctgtc cacggcatcc
    26161 gtttcgccag tgcccccatt ttttccatgt cgtgggtatg caaacgcgaa ataatcgcgc
    26221 ccgcgccgag aaatagcagc cctttgaaca gcgcatggtt gagcagatga aacagtgcgc
    26281 cgagcagtcc aaccacggtg agcagcgggt cgtgcagtga cagaccgacc atcgccacac
    26341 cgactgccag caaaataatg ccgacgtttt cgacggtact ccaggccagc agccgtttga
    26401 tatcctgttc cgccagcgca tacagcacgc ccaggagcgc ggagattgcg ccgatcgcca
    26461 tcaccagaat gccccaccac agcggcaaac ccgtttgcgc cagcagatcc atcgctactt
    26521 tcaggatgcc gaaaataccg attttgacca ttacgccaga catcaacgcc gaagcgtgcg
    26581 atggtgcggc agggtgagcg cgcggcaacc agctgtgcaa cggcatcatc ccggctttcg
    26641 cgccaaaacc gaaaaaggcc agcagaaaca ccgccgacgc cagccccgga gaaagtgaaa
    26701 gcgtgcgaaa ctggcaaaat cgaggctgcc gctttcgcgc cccatcagca agaaggcgat
    26761 cataatcagc accgatccgg cgtgggcgat aaaaaagtag agcatgccag cgttgatcga
    26821 cgttttatcc tgcctggcaa tgaccagaaa ccaggaagac agcgacatca tttcaaacag
    26881 cacgatgaac caaaaagcgt tgtccatcac cagcagggca accatcgatg cgatgaaaag
    26941 attcataaag aagcccatcg ccgccgcgcc tttgccctcg tattcgcgca tataagtcaa
    27001 tgaatagagc gaacaaacca ccaccagcaa ggagataacc agcaccataa atgccgagag
    27061 gctatccatt cgcagggtta aatcggcaaa gtcaaaaggg gtggcaaaat gggcgactaa
    27121 agttacacca gcatgtaatt gcgtaattcc gctgatgatg ccaatcacgc cgcccaccag
    27181 cgatgtgatg ccggaaagct taatagccag tctgtccaga ccgagtaaaa acagcgaagc
    27241 cagactggca aacagataga ggatcagcga ccaggttaat aattgcaggg catccattag
    27301 cgttgctcct gagtgaggga aaggggatcc tccccgccat tggcaaacca gcttgcggca
    27361 aggcgctgtt tttctttcat ctgacgttgc aggctatcgt cggtgatcag ccgtaacgcc
    27421 tgattcgggc aagcgcgaac gcaggccgga ccttctggca agaaatcaca caggtcgcat
    27481 ttcaccgcga cggtctggac accaggttcc cagcgcagca acgcatgttg gggcagcacg
    27541 ttttcttcgc cgtttttgag tgagccttca gcctgaaaaa catattgcgc atgggcattc
    27601 accggacggc ttcctgaagc gctgattgcg ccaaatgggc agaccacggc gcaaagcttg
    27661 cagccaatac agaggctttc gttgagttgg atcgcatcat ccctctgaga gatggcattg
    27721 accgggcaga cctgcaggca aggggcttcc tcacagtgat gacacacgac aggggcagtg
    27781 attgttgacg tcttcgccag ggccaggcgc gggtgttgct gtaaaccttg cgttttatgc
    27841 acgtccgaac aggcagcgag acaggtatta catcctgtac accacagtgg ttcggccacc
    27901 acaaagcggt tcatgtcggc tccttgaaaa tgatatgaaa cattgcattt catattgcat
    27961 aaaggatgcc attctgatta actcaatata ttcaataagt tatgtattaa ctggattctg
    28021 aacggtacaa aaatgatggt cattaacgtt cagcaaaaaa tggattcgac acccgtgtcg
    28081 caggggaaag cgagaatgtg atcttgatca cgagaagtga ggtatgctgg ccgcaagcgc
    28141 agccagcacg gaatggagca aagtaatcag gcgtgttctt tcagccagtt cagcacaacg
    28201 tcgtggtgat tgctggtttt gaaatcgtca aagacatgtt cgattttgcc atcagcgtca
    28261 atcaggaagc tgatgcgatg aatgccatcg taggttttgc ccatgaagga cttttcaccc
    28321 cagacgccga attgttcgca cacctggtgg tcctcatcag acaggagcgt aaagttaagc
    28381 agctcttttt ccgcaaaacg ggagagtttt tcgggtttat cggtgctgat acccagcaca
    28441 tcaacgcccg cttttttcaa ctcatccatg ttatcgcgta agccgcaggc ctgtacggta
    28501 cagccggggg tcatggcttt cgggtagaaa taaaccagaa cacgctgtcc ctggaagtcg
    28561 gtcaaattaa cttgttctcc gtcttgatcc ggcaagctaa atttcggtgc gatatcaccg
    28621 gctttcagtg gattcattac ttaactccat cctgttcatc atgttgggaa taattgacga
    28681 cgttaatact gccttgtgca ttgagttctg tacatagggc tttgaacgct tgctcaatat
    28741 ttgctgcgtc cgcagatgcg gggctgtggg cggttatctg aatatgcaac tgcgcagccc
    28801 gttcattttc agcaggttgc gtgcgcgaca ccagcttcgc aatgttcata tgatgcgcgt
    28861 cgaaaagtgc tgtgaagcgt tcaattaaat gcggggagtc tgccacatcg acctgaaccc
    28921 agacagatgc tggcattggc ggacgcggac gcgccgtcgt gcgcttcatc acgattaaaa
    28981 gatccagttc ggcacctttc aacggtaacg ttgattcaat cagagtaatg gcattccatg
    29041 aaccggaaag cagcataata aacgtgaact cttctcccag catcgccagg cgactgtctt
    29101 caatattaca gccgcaacta ctgacatgac gggtgatggt gttcacaatt ccagggcgat
    29161 cggcacccaa cgcagtgatc accagataat gttgcgatga cagtgtcaaa ctggttattc
    29221 ctttaagggg tgagttgttc ttaaggaaag cataaaaaaa acatgcatac aacaatcaga
    29281 acggttctgt ctgcttgctt ttaatgccat accaaacgta ccattgagac acttgtttgc
    29341 acagaggatg gcccatgttc acgggaagta ttgtcgcgat tgttactccg atggatgaaa
    29401 aaggtaatgt ctgtcgggct agcttgaaaa aactgattga ttatcatgtc gccagcggta
    29461 cttcggcgat cgtttctgtt ggcaccactg gcgagtccgc taccttaaat catgacgaac
    29521 atgctgatgt ggtgatgatg acgctggagc tggctgatgg gcgcattccg gtaattgccg
    29581 ggaccggcgc taacgctact gcggaagcca ttagcctgac gcagcgcttc aatgacagtg
    29641 gtatcgtcgg ctgcctgacg gtaacccctt actacaatcg tccgtcgcaa gaaggtttgt
    29701 atcagcattt caaagccatc gctgagcata ctgacctgcc gcaaattctg tataatgtgc
    29761 cgtcccgtac tggctgcgat ctgctcccgg aaacggtggg ccgtctggcg aaagtaaaaa
    29821 atattatcgg aatcaaagag gcaacaggga acttaacgcg tgtaaaccag atcaaagaac
    29881 tggtttcaga tgattttgtt ctgctgagcg gcgatgatgc gagcgcgctg gacttcatgc
    29941 aattgggcgg tcatggggtt atttccgtta cggctaacgt cgcagcgcgt gatatggccc
    30001 agatgtgcaa actggcagca gaagggcatt ttgccgaggc acgcgttatt aatcagcgtc
    30061 tgatgccatt acacaacaaa ctatttgtcg aacccaatcc aatcccggtg aaatgggcat
    30121 gtaaggaact gggtcttgtg gcgaccgata cgctgcgcct gccaatgaca ccaatcaccg
    30181 acagtggtcg tgagacggtc agagcggcgc ttaagcatgc cggtttgctg taaagtttag
    30241 ggagatttga tggcttactc tgttcaaaag tcgcgcctgg caaaggttgc gggtgtttcg
    30301 cttgttttat tactcgctgc ctgtagttct gactcacgct ataagcgtca ggtcagtggt
    30361 gatgaagcct acctggaagc ggcaccgctt gcggagcttc atgccccggc tggaatgatt
    30421 ttgccggtga cctccggtga ttatgcaatc ccggtgacca acggtagtgg tgctgtcggt
    30481 aaggcgctgg acattcgtcc accagcccag ccgctggcac tggtttctgg cgcgcgtacc
    30541 cagttcacgg gcgataccgc ttcattgctg gtggaaaatg gtcgtggcaa tactctgtgg
    30601 ccgcaggtgg ttagcgtgct gcaggcgaaa aactacacca tcacccaacg tgatgatgct
    30661 ggtcagacac tgaccaccga ttgggtacaa tggaaccgtc tggacgaaga cgagcagtat
    30721 cgtggtcgtt atcaaatctc tgttaagccg cagggttatc agcaggcggt tacggttaaa
    30781 ctgctgaacc tggaacaggc gggcaaaccg gttgcagacg cggcttccat gcagcgttac
    30841 agcacggaga tgatgaacgt tatttccgcc ggtctggata aatctgccac tgacgccgcg
    30901 aacgctgcgc aaaatcgtgc ctccaccact atggacgtac aaagtgcagc tgatgacacc
    30961 ggtttaccaa tgctggtcgt acgcgggccg ttcaatgtgg tttggcaacg tctgccagcg
    31021 gcgctggaaa aagtgggcat gaaagtgacc gacagcaccc gttcgcaggg caacatggcc
    31081 gtaacttata agccgctgtc tgacagcgac tggcaggaac tgggcgcgag cgatccaggc
    31141 ctggcatccg gtgactataa actgcaggtt ggcgatttag ataaccgcag cagcctacag
    31201 ttcatcgatc cgaaaggtca tactctgact cagagtcaga acgacgcgct ggtagctgtc
    31261 ttccaggctg cgtttagcaa gtaaaaatac agggctggaa tcatccggcc cttttttctg
    31321 atatgatacg caaacgtgtg cgtctgcagg aaaacgcgat tttagcggta attcgcacga
    31381 aatttgtttg tcggacgtag ttcggataag gcgttcacgc cgcatccgac aaaacatccg
    31441 gcacaccaga cagcaaaaga ttttaaaacg ttaattcaca cccaggagtg ataaagatgc
    31501 aaaagcaagc tgagttgtat cgtggtaaag cgaaaaccgt atacagcacg gaaaacccgg
    31561 acctgttggt gctcgaattc cgcaatgata cgtcagcagg ggatggcgcg cgcattgagc
    31621 agtttgatcg caaaggtatg gtgaacaaca agttcaacta cttcattatg agcaaactgg
    31681 ctgaagcggg tatcccgact caaatggagc gtctgctctc cgataccgaa tgtctggtga
    31741 aaaagctgga tatggtgccg gttgagtgtg tcgtgcgtaa ccgtgctgct ggctctctgg
    31801 tgaaacgtct tggaatcgaa gaaggtattg agctgaaccc gccgctgttc gatctgttcc
    31861 tgaaaaacga cgccatgcac gatccgatgg tcaacgaatc ttactgcgaa acctttggct
    31921 gggtgagcaa agagaacctg gcgcgtatga aagagctgac ctacaaagcg aacgatgtgc
    31981 tgaaaaaact gttcgatgat gctggtctga ttctggtcga cttcaagctg gagtttggtc
    32041 tgtacaaagg tgaagtggta ctgggcgatg agttttcccc ggacggcagc cgcctgtggg
    32101 acaaagaaac gctggagaaa atggacaaag accgtttccg ccagagcctc ggtggcctga
    32161 tcgaggccta tgaagccgtc gcccgccgcc tgggtgtaca gctggactga tttttctgtt
    32221 catcatcttg ccgtgcagct ggcacggcaa gacaaccgct ctcggaatat atgcattgaa
    32281 cgctcattcc tccgccattt catcccgctt tcctgtggta atcttcctct gaaccaccta
    32341 cgatcatttc tataatgaat atattgttga ggtatctatg cgttggcaag ggcgacgtga
    32401 aagtgacaat gttgaagaca ggcgcaacag ctctggtggt ccgtctatgg gcggtcccgg
    32461 ttttcgcctg ccgagcggta aaggcgggct gattttactg atagtcgtgc tggttgcagg
    32521 ctactatggc gttgatttaa ccgggttgat gaccgggcag ccggtttctc aacagcaatc
    32581 aacgcggtca attagcccaa atgaagacga agccgcaaaa ttcacctcgg tgattctggc
    32641 aaccacggaa gacacctggg gacaacagtt cgaaaagatg ggtaagacct atcagcaacc
    32701 gaagctggtc atgtaccgtg gaatgacgcg caccggctgc ggggcgggcc agtccattat
    32761 ggggccgttc tattgcccgg cggatggcac agtttatatc gatctctcct tctatgatga
    32821 catgaaagac aaacttggcg cggatggcga ttttgcccag gggtacgtta tcgcccatga
    32881 agtcggtcat catgtgcaga aactgttagg catcgagccg aaagttcgtc aactgcaaca
    32941 aaacgcgacg caggcggaag tgaaccgctt atctgtgcgt atggaactcc aggccgactg
    33001 ttttgccggt gtctgggggc atagtatgca gcagcaaggc gttctggaaa ccggcgatct
    33061 ggaagaggcg ctgaatgcgg cgcaggccat cggcgatgac cgtttacaac agcaaagtca
    33121 ggggcgagta gtaccagaca gtttcacaca tggcacttct cagcaacgct acagctggtt
    33181 taaacgtggt ttcgacagcg gcgatccggc acaatgtaat acttttggta aaagcattta
    33241 atttccgggg cagggatggc tgaactgact gcgctttaca cattaacagc gcaaatgaaa
    33301 cgtgaaggga tccgccgctt gctggtgttg agcggggaag agggttggtg ttttgatcat
    33361 gttcttaagt tgcgtgatgc cttacctggc gactggctgt ggatttcgcc gcagccagat
    33421 gctgaaaacc actgttcacc ctcggcgcta caaactttac ttgggcgcga gttccggcat
    33481 gcggtattcg acgcccgcca cggctttgat gccgctgcct ttgcagcact tagcggaacg
    33541 ttgaaagcgg gaagctggct ggttttgtta ctccctgtat gggaagagtg ggaaaaccaa
    33601 cctgatgccg actcgctgcg ctggagtgat tgccctgacc ctattgcgac gccgcatttt
    33661 gtccagcatc tcaaacgcgt acttacggcg gataacgacg ctatcctctg gcggcaaaac
    33721 cagccgttct cgttggcgca ttttactccc cgtactgact ggcaccccgc gaccggcgca
    33781 ccacaaccag aacaacagca actcttacag cagctactga ccatgccgcc gggcgtggca
    33841 gcggtaacgg ctgcgcgtgg gcgcggtaag tcggcgctgg cagggcaact catttctcgt
    33901 attgcgggca gtgcgattgt caccgcgccc gcaaaagcgg caacggatgt actggcacaa
    33961 tttgcgggcg agaagtttcg ctttattgcg ccggatgcct tgttagccag cgatgagcaa
    34021 gccgactggc tggtggtcga cgaagccgca gccatacctg cgccgttgtt gcatcaactg
    34081 gtatcgcgtt ttcctcgaac gttgttaacc actacggtgc agggctacga aggcaccgga
    34141 cgtggttttt tgctgaaatt ttgcgctcgc tttccgcatt tacaccgttt tgaactgcaa
    34201 cagccgatcc gctgggcaca gggatgcccg ctggaaaaaa tggttagtga ggcactggtt
    34261 tttgacgatg aaaacttcac ccatacacca caaggcaata tcgtcatttc cgcatttgaa
    34321 caaacgttat ggcgaagcga gccagaaacg ccgttaaagg tttatcagtt attgtctggt
    34381 gcgcactacc ggacttcgcc gctggattta cgccggatga tggatgcacc agggcaatat
    34441 tttttacagg cggctggcga aaacgagatt gccggagcgc tgtggctggt ggatgagggg
    34501 ggattatctc aacaactcag tcaggcggta tgggcaggtt ttcgtcgccc gcggggtaat
    34561 ctggtggccc agtcgctggc ggcgcacggt agcaatccac tggcagcgac attgcgtgga
    34621 cggcgggtca gccggatagc agttcatccg gcgcgtcagc gggaaggcac agggcagcaa
    34681 cttattgctg gtgctttgca atatacgcgt gacctcgact atctttcggt gagttttggt
    34741 tacaccgggg agttatggcg tttctggcac cgctgcggtt ttgtgctggt gcggatgggt
    34801 aatcatcgtg aagccagcag cggttgctat acggcgatgg cgctgttacc gatgagtaat
    34861 gcgggtaaac aactggctga acgtgagcat taccgtttac gtcgcgatgc gcaagctctc
    34921 gcaaagtgga atggcgaaac gcttcctgtt gatccactaa acgatgccgt cctttctgac
    34981 gacgactggc ttgaactggc cggttttgct ttcgctcatc gtccgctatt aacatcgtta
    35041 ggttgcttat tgcgtctgct acaaaccagt gaactggcat taccggcgct gcgtgggcgt
    35101 ttacagaaaa acgtcagcga cgcgcagtta tgtaccacac ttaaactttc aggccgcaag
    35161 atgttactgg tccgtcagcg ggaagaggcc gcgcaggcgc tgttcgcact taatgatgtt
    35221 cgcactgagc gtctgcgcga tcgcataacg caatggcaat tttttcactg actccttcaa
    35281 ttttctccca tggtcattgt gagtcagcgg cgtaaaatta atgacatcag ttaaggagtt
    35341 cgccatgaaa catgaccatt ttgttgttca aagcccggat aaacctgcgc aacagttgtt
    35401 actgcttttc cacggtgtcg gggataaccc ggtggcaatg ggggaaatag gtagctggtt
    35461 tgcaccgctg tttcctgatg cgctggtggt gagtgtcggt ggcgcggagc cgagcggtaa
    35521 tccggcgggg cgtcagtggt tttcggtgca gggtattacg gaagataatc gccaggcgcg
    35581 tgtggatgcg atcatgccga cgtttattga aacggtgcgc tactggcaga aacagagcgg
    35641 agtaggggct aacgccacgg cgctcatcgg tttctctcaa ggcgcgatta tggtgctgga
    35701 gagcattaaa gccgaaccgg gccttgcttc acgcgtcatt gcttttaacg gacgctatgc
    35761 gagcctgccg gaaactgcgt ctacagccac gacaattcac ctgattcatg gcggtgaaga
    35821 tccggttatc gatctggcgc atgcggttgc ggcgcaggaa gcgttaatca gtgccggtgg
    35881 tgacgtgacg ctggatatcg tggaggattt aggccatgcg atcgacaatc gcagtatgca
    35941 atttgccctg gatcacttgc gttataccat tccgaaacat tatttcgacg aagcgctaag
    36001 tggcggtaag cctggcgatg atgatgtgat tgaaatgatg taaaagacag attacctggc
    36061 ctgataagac gcgtgagcgt cgcatcaggc atcgttcacc attgccggat gcggcgttaa
    36121 cgccttatcc ggcctacggt tgagcactca gcttacttct tcggttgatc ctttttcggc
    36181 cagtcgtcgt catcgtccca cttatcgttg aaatcacgat gcgggggaag ctccggcttg
    36241 ttggcgagaa atttcttatg gtccacgcgc ttgagatctt taatcacgtt cagcaggacg
    36301 cctaccaaaa agacaatcac cagaatccac caatatttag ccagccagtc catgcttatt
    36361 tcctcttgca gaaccactca tcaggcgacg agctgttcca tgatacgttg atacatacgg
    36421 gcaagtagct gcaggtcggc agcgttcaca cattcattaa ttttatgaat agtggcattg
    36481 accggcccga gttccaccac ctgcgccccc atgcgagcaa taaagcgccc gtcggacgtt
    36541 ccgcctgtgg tcagtagctg cggtttaatt tcattatagt gctcaaccgc gttaacgacc
    36601 gcatccacca gtttaccgcg cgcggtcaaa aatggctgcc cggaaagcca ccagtccacc
    36661 gtataaagca gttgatgttt ttcaagcagg gcaagcacct gtgctttgat catctcatca
    36721 gtcagttcgg tgctgaagcg gaagttaaac tgcacaaaca gttcacccgg aataacgttg
    36781 ttactgcccg ttcccgcctg aatattagca atctgcatac tggtcgccgg gaaaaattca
    36841 ttgccctgat cccactcaat agccactaat tcattaagga aaggggctgc gcgatgtact
    36901 ggattgtcag ccagatgcgg gtaggccaca tgcccctgaa cgccatgaat ggtaaggttg
    36961 caggttaacg atccgcgacg accatttttc accacatcac caaccacttc gatactcgac
    37021 ggttcgccga ccaggcagta atcgagacgc tcattacgcg ccattaacgc ttcgacgact
    37081 tttaccgtac cgttgtgggc actagcttct tcatcagagg tgatcagaaa tgccagtcgc
    37141 cccgtatggt tgggatgttg tgcgacaaaa cgttctgccg ctaccaccat cgccgccagc
    37201 gagcctttca tatctgccgc accgcgcccg aataacatgc cgtcacgaat ggttggttca
    37261 aacggcggat tgatccaacg atcggcgtcg ccaggcggca ccacgtcggt atgcccggca
    37321 aaggccaatg tttcaccttg cccacgccat gcccaaaaat tctgcgtatc ggcaaagtcc
    37381 atgcgttcaa cggtaaaacc gatcgcctgc aaacgttcaa tcaacaaagc ctggcatcct
    37441 gcgtcatcag gactcaggga agggcggcga ataagctgtt gtgtcagctc aataaccggg
    37501 cacgacatag actacacctc atggaaaaat tgctgataac tggaatcact gaaacccagc
    37561 agcataggct taccgggcgc gcagagcaat ggacgtttga taattgcagg catttcagtc
    37621 attaatgccg ccgcagaggc cgcatcggtg attttattgc gggtggtttc gtccagttta
    37681 cgccaggttg taccacgggt gttgagtaac gcttcccagc ctaattcgtt gataaaaccg
    37741 ttcaataatt cgctgtccag cccatcgacg cggtaatcat gaaaacgata gtcgatgtta
    37801 ttggcttcca gccaacggcg agccttttta atggtgtcac aatttttgat gccgtaaagt
    37861 gtaaccataa tgaatccttt ttcgatttat gagaataaat actcatttaa gggtgaaaac
    37921 attttgcctg caaatgatta tcaggaaata taa
//
LOCUS       DAJOSY010000032        37896 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJOSY010000032 DAJOSY010000000
VERSION     DAJOSY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.032"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1870)
                     /locus_tag="OHN27_001745"
     CDS             complement(<1..1870)
                     /locus_tag="OHN27_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286197.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVLRLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSNTQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTAGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGNQLNEENPHQLQQLI
                     RLPGQQYDEESGLYYNRHRYYDPLRGRYITQDPIGLKGGWNLYTYPLSPVNSMDPLGL
                     YEFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDL"
     gene            complement(2030..2242)
                     /gene="rpmE"
                     /locus_tag="OHN27_001746"
     CDS             complement(2030..2242)
                     /gene="rpmE"
                     /locus_tag="OHN27_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCQ2286198.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            2445..4643
                     /gene="priA"
                     /locus_tag="OHN27_001747"
     CDS             2445..4643
                     /gene="priA"
                     /locus_tag="OHN27_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCQ2286199.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            4799..5824
                     /gene="cytR"
                     /locus_tag="OHN27_001748"
     CDS             4799..5824
                     /gene="cytR"
                     /locus_tag="OHN27_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCQ2286200.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            5916..6875
                     /gene="ftsN"
                     /locus_tag="OHN27_001749"
     CDS             5916..6875
                     /gene="ftsN"
                     /locus_tag="OHN27_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCQ2286201.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASSQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            6968..7498
                     /gene="hslV"
                     /locus_tag="OHN27_001750"
     CDS             6968..7498
                     /gene="hslV"
                     /locus_tag="OHN27_001750"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCQ2286202.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            7508..8839
                     /gene="hslU"
                     /locus_tag="OHN27_001751"
     CDS             7508..8839
                     /gene="hslU"
                     /locus_tag="OHN27_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCQ2286203.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            8906..9832
                     /gene="menA"
                     /locus_tag="OHN27_001752"
     CDS             8906..9832
                     /gene="menA"
                     /locus_tag="OHN27_001752"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCQ2286204.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            9925..10410
                     /gene="rraA"
                     /locus_tag="OHN27_001753"
     CDS             9925..10410
                     /gene="rraA"
                     /locus_tag="OHN27_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCQ2286205.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(10495..10740)
                     /gene="zapB"
                     /locus_tag="OHN27_001754"
     CDS             complement(10495..10740)
                     /gene="zapB"
                     /locus_tag="OHN27_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCQ2286206.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            11165..12010
                     /gene="glpF"
                     /locus_tag="OHN27_001755"
     CDS             11165..12010
                     /gene="glpF"
                     /locus_tag="OHN27_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCQ2286207.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            12033..13541
                     /gene="glpK"
                     /locus_tag="OHN27_001756"
     CDS             12033..13541
                     /gene="glpK"
                     /locus_tag="OHN27_001756"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCQ2286208.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            13676..14686
                     /gene="glpX"
                     /locus_tag="OHN27_001757"
     CDS             13676..14686
                     /gene="glpX"
                     /locus_tag="OHN27_001757"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCQ2286209.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            14783..15529
                     /gene="fpr"
                     /locus_tag="OHN27_001758"
     CDS             14783..15529
                     /gene="fpr"
                     /locus_tag="OHN27_001758"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCQ2286210.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPHCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESTIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(15534..15962)
                     /gene="uspD"
                     /locus_tag="OHN27_001759"
     CDS             complement(15534..15962)
                     /gene="uspD"
                     /locus_tag="OHN27_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCQ2286211.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(15989..16288)
                     /locus_tag="OHN27_001760"
     CDS             complement(15989..16288)
                     /locus_tag="OHN27_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCQ2286212.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAATSSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(16500..16940)
                     /locus_tag="OHN27_001761"
     CDS             complement(16500..16940)
                     /locus_tag="OHN27_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCQ2286213.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            17041..17640
                     /locus_tag="OHN27_001762"
     CDS             17041..17640
                     /locus_tag="OHN27_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCQ2286214.1"
                     /translation="MKPGCTLFFLLCSALTVTTAAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            17748..18515
                     /gene="tpiA"
                     /locus_tag="OHN27_001763"
     CDS             17748..18515
                     /gene="tpiA"
                     /locus_tag="OHN27_001763"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCQ2286215.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(18570..19325)
                     /gene="cdh"
                     /locus_tag="OHN27_001764"
     CDS             complement(18570..19325)
                     /gene="cdh"
                     /locus_tag="OHN27_001764"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCQ2286216.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGRYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(19432..20421)
                     /gene="sbp"
                     /locus_tag="OHN27_001765"
     CDS             complement(19432..20421)
                     /gene="sbp"
                     /locus_tag="OHN27_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCQ2286217.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(20741..21703)
                     /gene="pfkA"
                     /locus_tag="OHN27_001766"
     CDS             complement(20741..21703)
                     /gene="pfkA"
                     /locus_tag="OHN27_001766"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCQ2286218.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(21884..22786)
                     /gene="fieF"
                     /locus_tag="OHN27_001767"
     CDS             complement(21884..22786)
                     /gene="fieF"
                     /locus_tag="OHN27_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCQ2286219.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(22935..23435)
                     /gene="cpxP"
                     /locus_tag="OHN27_001768"
     CDS             complement(22935..23435)
                     /gene="cpxP"
                     /locus_tag="OHN27_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCQ2286220.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            23585..24283
                     /gene="cpxR"
                     /locus_tag="OHN27_001769"
     CDS             23585..24283
                     /gene="cpxR"
                     /locus_tag="OHN27_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCQ2286221.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            24280..25653
                     /gene="cpxA"
                     /locus_tag="OHN27_001770"
     CDS             24280..25653
                     /gene="cpxA"
                     /locus_tag="OHN27_001770"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCQ2286222.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETLKANQLWGEVLDNAAFEAEQMGKSFSV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(25759..26433)
                     /gene="yiiM"
                     /locus_tag="OHN27_001771"
     CDS             complement(25759..26433)
                     /gene="yiiM"
                     /locus_tag="OHN27_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCQ2286223.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(26582..27565)
                     /gene="kdgT"
                     /locus_tag="OHN27_001772"
     CDS             complement(26582..27565)
                     /gene="kdgT"
                     /locus_tag="OHN27_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCQ2286224.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGAFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(27825..28445)
                     /gene="sodA"
                     /locus_tag="OHN27_001773"
     CDS             complement(27825..28445)
                     /gene="sodA"
                     /locus_tag="OHN27_001773"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCQ2286225.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            28730..29764
                     /gene="rhaT"
                     /locus_tag="OHN27_001774"
     CDS             28730..29764
                     /gene="rhaT"
                     /locus_tag="OHN27_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCQ2286226.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLISTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKSLIIHNVLLSTLGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(29761..30627)
                     /gene="rhaR"
                     /locus_tag="OHN27_001775"
     CDS             complement(29761..30627)
                     /gene="rhaR"
                     /locus_tag="OHN27_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCQ2286227.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(30683..31519)
                     /gene="rhaS"
                     /locus_tag="OHN27_001776"
     CDS             complement(30683..31519)
                     /gene="rhaS"
                     /locus_tag="OHN27_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCQ2286228.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            31807..33276
                     /gene="rhaB"
                     /locus_tag="OHN27_001777"
     CDS             31807..33276
                     /gene="rhaB"
                     /locus_tag="OHN27_001777"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCQ2286229.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQEQQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            33273..34532
                     /gene="rhaA"
                     /locus_tag="OHN27_001778"
     CDS             33273..34532
                     /gene="rhaA"
                     /locus_tag="OHN27_001778"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCQ2286230.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSRRG"
     gene            34983..35807
                     /gene="rhaD"
                     /locus_tag="OHN27_001779"
     CDS             34983..35807
                     /gene="rhaD"
                     /locus_tag="OHN27_001779"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCQ2286231.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDEADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGLGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            35817..36131
                     /gene="rhaM"
                     /locus_tag="OHN27_001780"
     CDS             35817..36131
                     /gene="rhaM"
                     /locus_tag="OHN27_001780"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCQ2286232.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(36172..37566)
                     /locus_tag="OHN27_001781"
     CDS             complement(36172..37566)
                     /locus_tag="OHN27_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCQ2286233.1"
                     /translation="MKKSILSLAIGLLLTCSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPGNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYSIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         9208 a         9936 c         9772 g         8980 t
ORIGIN      
        1 gcaagtctac actatcatta tcatttgaac ttatcggatt catggggaaa tattcacctt
       61 gcttcttaca tattagtcca ccatattctt tattctcgtt aattgattct ccattacaca
      121 ttgccaatgc aaatattcca atatcatcta tattttttga tttaaattca tataatccta
      181 atggatccat gctattcacc gggctcagcg gatatgtata caggttccat ccccccttca
      241 gcccaatcgg atcctgagtg atatacctcc cccgcagcgg gtcataatag cggtggcggt
      301 tataatacag gccggactcc tcatcatact gctgccccgg caggcggatg agctgctgca
      361 gctggtgcgg gttctcttca ttcagctggt taccccattc atcgtattcc cggcgtattt
      421 ccagcccgtc aggcccggtg gctgacgtta actggctgtg gtggttatag taaaacgccg
      481 atgccctgcc atccggcgtg gtgatgcgcg tgatgaggcc cgtcaccaca tccgggctgt
      541 attctgttgt cctgcctgcg gcatccgtct gtgccctgag cctgcccacc gcgtcaaact
      601 gactctgcgt gacgctgccg tccgcgtgtt cctttttcac cacccgcttc agcccgcctt
      661 cgcctgcggt atgcaggact tcacggcggt tcaggctgtc ggtgatggtg atgcggtctt
      721 tctcatactg atacgtgtag cttaagcctg ccgggtttag ctgttctgtc acccgcccgt
      781 cgctgtcgta acggtaacgg atttccggcc ggcccgtgtg acggtgcgcc accatccggc
      841 cccggtattt atcatcgtaa gtaaagctgc gcacctgtgt attgctgcgg tcatacacca
      901 ccgccagttc gccgcgcggc gtccagccat agcgtaccag cggcgcggca ggtaaattct
      961 ccgggtattc cgggtcgtgc gtcagccaca cggcagacag acggatgccg ttgtcccggc
     1021 catattcggt gtaacccggc agggtatcag gaaaagcgga cggttccgtc ccgccggaaa
     1081 tggcctgctg ccgggcttct tctgcccgct gcgcctgcgt ggtcagtacc agccggaagt
     1141 gacgcccggc accatccgtc acgccggtga tttcgccgct gaattcaccg gcggcttcgc
     1201 ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc cggtacggcg
     1261 gcagcggcgc aggcagcacc tcatccgctt ccggcacccg ctcacaccag ccgagcagcc
     1321 accacggccc ctgcggactg tttgtcgcca gataacgatg cggacttaag cggagttctt
     1381 ccggcagcgc ctgccagagt gcggccagcc ggtgaccttc atccagtctc aggacgccgc
     1441 cgcgcaccag ccacagtgac tcgctgcggc tgtaaccgtc ctcaccggga aacaggtgct
     1501 caaaatacag gcttctgccg ccgttatcac tgagtatcag tgtgttatcg cgcagctgta
     1561 agcggatatc cgcaggcatt ttccagccgg ggccgaggct ccccaccggc gcgggcgttt
     1621 ttgtccggta actgctgtag gtgcgggaga ggatgaacgg cagcgggccg ggcagggcaa
     1681 ggtcggtttc accgggaagg acctttgcac cgagcagggg attgaccgga tggccggacg
     1741 tcactccgcc ggggcacacc gaacaggcca cgccggtggg ggcaccaatg cgcaccccgg
     1801 ctgaaccctg aacaatgcta ccgccatact gcgtcatgtc gccctgacgc gctgccggtt
     1861 ttccgctcat cgtaaaatcc ttttgaaata aataagaacc agaagcctag cgccagtcaa
     1921 caacaggaaa tgataatgag ttaacacccc ggaatttttc agaatttata aaaggcaaat
     1981 tacaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga aatccggtct tatttgctgc
     2041 ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc accggtagca acatcacgct
     2101 gtttgccagt gaagaatggg tggcacttgc tgcacacgtc gaggttcagg tcatgaccaa
     2161 cggtggagcg gattttcatt acgttaccgc aagagcagct agcagtaatt tcttcgtatt
     2221 tcgggtgaat atcttttttc atgggaaaac ctcggtttaa ggccgcgtcg ctcttccagc
     2281 cctaacgcca gacaccacgc gatgttaaaa gtatagcttc aatacgatca tttcgtacga
     2341 aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac tgatccgcac tcttctacgg
     2401 caatgtgtat actaacccac cgaatttcaa gtcaggatga tgctatgccc gttgcccacg
     2461 ttgccttgcc cgttccgctt cctcgtacct ttgactatct gctgccagaa ggcatgacgg
     2521 ttaaagccgg gtgtcgcgtg cgcgtgccgt ttggcaaaca gcaggagcgc atcggggttg
     2581 tggtgtcagt tagcgatgtc agtgagctgc cgcttaacga gctgaaagcg gtagtggaag
     2641 tgctggacgt tgagccggta tttactcact ccgtctggcg gttactgctc tgggcggcag
     2701 attactatca tcatccgatt ggcgatgtgc tgtttcatgc cttgccgatt ttactgcgcc
     2761 aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt tgccactgaa caaggccagg
     2821 cggtggatct gaacagcctg aaacgctccc ccaagcaaca acaggcgctg gcggcgttac
     2881 ggcaaggcaa aatctggcgc gaccaggtcg ccacgctcga atttaatgat gccgcgttgc
     2941 aggcgctacg caaaaaaggt ctgtgtgatt tagcaagtga aacaccagag tttagcgact
     3001 ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt gaataccgaa caggccaccg
     3061 ccgttggcgc aattcatagc gcggcagata ctttttctgc ctggctgctg gcgggcgtta
     3121 ccggttccgg taaaacggag gtttatctca gtgtactgga aaacgtgctc gctcagggca
     3181 aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc gcaaactatc gcccgttttc
     3241 gtgaacgttt taatgccccc gtggaagttc tgcattccgg cctgaacgac agcgagcgtc
     3301 tttcggcgtg gttgaaagcg aaaaatggcg aggcggcgat tgtcatcggc acccgctccg
     3361 cgctgtttac gccgtttaaa aatctcgggg tgattgtcat tgatgaagag cacgacagct
     3421 cctacaagca gcaggaaggc tggcgctatc atgcccgcga cctggcggtt tatcgtgcgc
     3481 acagcgagca aatcccgatt attcttggct ctgcaacacc cgcactggaa acgttatgca
     3541 acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg tcgggcgggg aatgcgcgtc
     3601 cggcaattca acacgtgctg gatttaaaag gtcagaaggt gcaggcgggg ctggctccgg
     3661 cgttaatcac ccgtatgcgc cagcatttac aggccgacaa ccaggttatc ctctttctta
     3721 accgccgtgg ctttgcgcct gccttgctgt gccacgactg cggctggatt gccgaatgtc
     3781 cacgttgcga tcactactac acgctgcatc aggcgcagca acatctgcgc tgccaccact
     3841 gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg cggttccacg cacctggtac
     3901 ccgtagggct gggcaccgaa cagcttgagc aaacgctcgc gccgctgttc cctggtgtgc
     3961 ccatttctcg tatcgaccgc gataccacca gccgcaaagg ggcgctggaa cagcaactgg
     4021 cggaagtgca tcgcggcggc gcgaggatct tgattggcac gcaaatgctg gcgaaaggtc
     4081 accactttcc ggatgtgacg ctggtggcgt tactggatgt ggacggcgcg ctgttttctg
     4141 ccgattttcg ctcggcagaa cgtttcgccc agctttacac ccaggtcgcc ggtcgtgccg
     4201 ggcgtgcggg taaacagggc gaagtggtgc tgcaaacgca ccatccggaa catcctctgt
     4261 tgcaaacgtt gctctataaa ggctacgacg cctttgccga acaggcgctg gctgagcgac
     4321 gcatgatgca gctgccgccg tggaccagcc atgtgattgt gcgtgcggaa gatcataaca
     4381 atcagcacgc gccgctgttc ctgcaacaac tgcgtaatct gatcctctcc agcccgctgg
     4441 cagacgacaa actgtgggtt ctcggcccgg ttccggctct ggcacctaag cgtggtggcc
     4501 gctggcgctg gcagatcttg ttgcagcacc cttcccgcgt gcgcttgcaa cacatcatta
     4561 gtggtacgct ggcgcttatt aatacaatac cggattcccg caaggtgaaa tgggtgttgg
     4621 atgtcgatcc catcgaaggc taatgcgcaa acgttgcgag gcggatcgaa aaattcaata
     4681 ttcatcacac ttttcatgaa aattctgtaa ccgttttcac gcgctatctg ctaaaaatgt
     4741 tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt gccaggcgag gagtgagtgt
     4801 gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac gttgccctca aggcaaaagt
     4861 ctctacagcg accgtctccc gagcattaat gaatcccgat aaagtctccc aggccacccg
     4921 taatcgggtt gaaaaagcgg cccgggaagt gggttattta ccgcagccta tggggcgcaa
     4981 cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc ccggatatct gcgatccctt
     5041 ctttagcgaa attattcgcg gtatcgaagt tacggcggca aatcacggat atctggtgct
     5101 gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac
     5161 caagcaaatt gatggcatgt tgttgctggg ttcaaggctg ccgtttgatg ccagcattga
     5221 ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa tttgcaccgg agctggagct
     5281 gcctacagtt catatcgaca atctgaccgc cgcatttgat gcagtaaatt atttatatga
     5341 gcaagggcat aaacggattg gctgtatagc cggtcccgaa gagatgccgc tgtgtcacta
     5401 ccgcctgcaa ggctatgttc aggcgctgcg tcgctgcggc attatggttg atccgcaata
     5461 catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa gcgatgcagc agctgcttga
     5521 tcttccacaa ccgcctactg ctgtcttctg ccatagcgat gtgatggcgc tcggcgcact
     5581 ttctcaggca aaacgccagg ggctgaaagt cccggaagac ctttccataa tcggttttga
     5641 taacatcgac ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc agccgcgtta
     5701 cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa atgcaggggc aacacgttgg
     5761 cagtggctct cgtttaatgg actgcgaact tatcatccgg ggatcaacac gcgcgttacc
     5821 ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc ccgccgctta agtaacatgg
     5881 cgggctgacg aacgaataaa tacagcgaaa cgatagtggc acaacgagat tatgtacgcc
     5941 gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag caacgaaatc
     6001 tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc cgttcttgtg acctttatcg
     6061 gtggtctgta cttcattacg catcacaaga aagaagagtc cgagacgctg caaagccaga
     6121 aagtgaccgg aaacggacta ccaccaaaac cagaagaacg ctggcgctac attaaagagc
     6181 tggaaagtcg ccagccggga gtgcgtgcgc ccacagaacc ttctgccggt ggtgaagtga
     6241 aaacgccgga gcaactgaca ccagaacaac gtcagcttct tgaacaaatg caggctgata
     6301 tgcgccagca gccaacgcag ctggttgaag ttccgtggaa tgaacagacg ccggaacagc
     6361 gtcagcaaac gctacagcgc caacgtcagg cgcagcaatt agcggaacag caacgcctgg
     6421 ctcagcagtc gcgtacgact gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg
     6481 caccggtaca ggcgcaaccg cgccagtcta aaccggcttc ttctcagcag ccgtaccagg
     6541 atctgctgca aactcctgcg cacacgactg cgcagtcgaa gccgcagcaa gctgcgccag
     6601 ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa aaaagacgaa cgccgctgga
     6661 tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga gacggtacgt gctcagctgg
     6721 cgttcgaagg ctttgactcg aaaatcacca ccaacaatgg ctggaatcgt gtggtcattg
     6781 gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa tcggttgaag atggcgggtc
     6841 atacaaactg cattcggctc gccgccgggg gttgaaaccc tcaaaatccc ccccatctat
     6901 aattgcatta tgccccgtac ttttgtacgg ggtttgtact ctgtattcgt aaccaagggg
     6961 tcagctcgtg acaactatag taagcgtacg ccgtaacggc catgtggtca tcgctggtga
     7021 tggtcaggcc acgttgggca ataccgtaat gaaaggcaac gtgaaaaagg tccgccgtct
     7081 gtacaacgac aaagtcatcg cgggctttgc gggcggtact gcggatgctt ttacgctgtt
     7141 cgaactgttt gaacgtaaac tggaaatgca tcagggccat ctggtcaaag ccgccgttga
     7201 gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa cttgaagcac tgctggcagt
     7261 cgcggatgaa actgcatcgc ttatcatcac cggtaacggt gacgtggtgc agccagaaaa
     7321 cgatcttatt gctatcggct ccggcggccc ttacgcccag gctgcggcgc gcgcgctgtt
     7381 agaaaacact gaacttagcg cccgtgaaat tgctgaaaag gcgttggata ttgcaggcga
     7441 catttgcatc tataccaacc atttccacac catcgaagaa ttaagctaca aagcgtaagg
     7501 atctcccatg tctgaaatga ccccacgcga aatcgtcagc gaactggata agcacatcat
     7561 cggccaggac aacgccaagc gttctgtggc gattgctctg cgtaaccgct ggcgtcgcat
     7621 gcagctcaac gaagagctgc gccatgaagt gaccccgaaa aatatcctga tgatcggccc
     7681 gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct aagctggcga atgcgccgtt
     7741 catcaaagtt gaagcgacca aattcaccga agtgggctac gtcggtaagg aagtggattc
     7801 tattattcgc gatctgaccg atgccgccgt gaaaatggta cgcgtccagg ctatcgagaa
     7861 aaaccgttat cgcgctgaag aactggcaga agaacgtatt ctcgacgtgc tgatcccacc
     7921 ggctaaaaac aactggggac agaccgaaca gcagcaggaa ccgtccgctg ctcgtcaggc
     7981 attccgcaaa aaactgcgtg aaggccagct tgatgacaaa gaaatcgaga tcgatcttgc
     8041 cgcagcaccg atgggcgttg aaattatggc tcctccgggc atggaagaga tgaccagcca
     8101 gctgcagtcc atgttccaga acctgggcgg ccagaagcaa aaagcgcgta agctgaaaat
     8161 caaagacgcc atgaagctgc tgattgaaga agaagcggcg aaactggtga acccggaaga
     8221 gctgaagcaa gacgctatcg acgctgttga gcagcacggg atcgtgttta tcgacgaaat
     8281 cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat gtttctcgtg aaggtgttca
     8341 gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc accaaacacg ggatggtcaa
     8401 aactgaccac attctgttta tcgcttctgg cgcgttccag attgcgaaac cgtctgacct
     8461 gatcccggaa ctgcaaggtc gtctgccaat ccgcgttgaa ctgcaggcgc tgaccaccag
     8521 cgacttcgag cgtattctga ccgagccgaa tgcctctatc accgtgcagt acaaagcact
     8581 gatggcgact gaaggcgtaa atatcgagtt taccgactcc ggtattaaac gcatcgcgga
     8641 agcggcatgg caggtgaacg aatctaccga aaacatcggt gctcgtcgtt tacacactgt
     8701 tctggagcgt ttaatggaag agatttccta cgacgccagc gatttaagcg gtcaaaatat
     8761 cactattgac gcagattatg tgagcaaaca tctggatgcg ttggtggcag atgaagatct
     8821 gagccgtttt atcctataat cgcgttcaat cattttcatc attgtttgat ggggctgaaa
     8881 ggccccattt ttattggcgc gtattatgac tgaacaacaa attagccgaa ctcaggcgtg
     8941 gctggaaagt ttacgaccta aaaccctccc cctcgccttt gctgcaatta tcgtcgggac
     9001 agcgctggca tggtggcaag gtcacttcga tccgctggtc gccctgctgg cactaattac
     9061 cgccgggcta ttacagatcc tttctaacct cgccaatgat tacggcgatg cggtaaaagg
     9121 cagcgataaa cctgaccgca ttgggccgct acgcggcatg caaaaagggg tcattaccca
     9181 gcaagagatg aaacgggcgc tcattattac cgtcgtgctc atctgtctct ccgggctggc
     9241 actggttgca gtggcatgcc atacgctggc cgattttgtc ggtttcctga ttcttggcgg
     9301 gttgtcgatc attgccgcta tcacctacac cgtgggcaat cgtccttatg gttatatcgg
     9361 tctgggtgat atttccgtac tggttttctt tggctggttg agtgtcatgg ggagctggta
     9421 tttacaggct catacattga ttccggcact gatccttccg gcgaccgcat gcggcctgct
     9481 ggcaacggca gtactgaata ttaataacct gcgtgatatc aatagcgacc gcgaaaatgg
     9541 caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg cgtcgttatc atgcctgcct
     9601 gctgatgggc tcgctggtgt gtctggcgct gtttaatctc ttttcgctgc atagcctgtg
     9661 gggctggctg ttcctgctgg cggcaccatt actggtgaag caagcccgtt atgtgatgcg
     9721 ggaaatggac ccggtggcga tgcgaccaat gctggaacgt actgtcaagg gagcgttact
     9781 gactaacctg ctgtttgttt tagggatatt cctaagccag tgggcagcat aactgacaaa
     9841 tatcaattaa caattgatga ttttgccaac agcccacata gcgcgatata ctgaaaattc
     9901 tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt ccgagctttg tgacatctat
     9961 caagaagatg ttaacgtcgt ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt
    10021 ggcggacaaa taatcacggt aaaatgtttc gaggacaacg ggttgctgta cgatctgctc
    10081 gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca
    10141 ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg aatgggaagg tctggtcatt
    10201 tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg atatcggcat ccaggcgatg
    10261 gcggcaattc cggttggtgc cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat
    10321 tttggcggtg tcaccttctt ctccggcgac catctttatg ccgacaatac cgggattatt
    10381 ctttcagaag atccgctgga tattgaatga taagaaaggc accgcaaggt gccttttttc
    10441 tgcgttacct gttggcctac acagtaaaga aattacgcgg aagatgaagc gtaatcagac
    10501 ctcttccatg cgacccagca gggcctgcag acgttcctgc cagccgttct gctgttcttt
    10561 cagatggttg ttctcacgct ccagctcttc gcgctgatgc tgggcatttt gaacttcctg
    10621 cgacagtgag ttgttttttt ctttcagctc ttcgatttcc atctgcaaca gagtgatggt
    10681 atcaatcgcc tgctgtactt ttgcttccag tttctcaaac acttctaatg acattgtcat
    10741 acctctcctg aattgcaagg cgttgatgga taaaaatcct cgtcccgatt accggtgacg
    10801 ccttaataaa tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg
    10861 tccagcggca cgccttgcag attacggttt gccacacttt tcatccttct cctggtgaca
    10921 taatccacat caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc
    10981 atcatgtaca atcagatgga ataaatggcg cgataacgct cattttatga cgaggcacac
    11041 acattttaag ttcgatattt ctcgtttttg ctcgttaacg ataagtttac agcatgccta
    11101 caagcatcgt ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc
    11161 gattatgagt caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg
    11221 gttgttgatt ttcttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt
    11281 tggtcagtgg gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac
    11341 cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc attgcattgt ggctgtttgc
    11401 ctgtttcgac aagcgcaaag ttattccttt tatcgtttca caagttgccg gcgctttctg
    11461 tgctgcggct ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca
    11521 tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct ggcactttct ctacttaccc
    11581 taatcctcat atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct
    11641 gatggggctg atcctggcgt taacggacga tggcaacggt gtaccacgcg gccctttggc
    11701 tcccttgctg attggtctac tgattgcggt cattggcgca tctatgggcc cattgacagg
    11761 ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg
    11821 gggcaatgtc gcctttaccg gcggcagaga cattccttac ttcctggtgc cgcttttcgg
    11881 ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc aaactgattg gtcgccattt
    11941 gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca actccttcag aacaaaaagc
    12001 ttcgctgtaa tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc
    12061 tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta
    12121 gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc aggttgggta gaacacgacc
    12181 caatggaaat ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata
    12241 tcagttccga tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct
    12301 gggaaaaaga aaccggcaag cctatctata acgccattgt ctggcagtgc cgtcgtaccg
    12361 cagaaatctg cgagcattta aaacgtgacg gtttagaaga ttatatccgc agcaataccg
    12421 gtctggtgat tgacccgtac ttttctggca ccaaagtgaa gtggatcctc gaccatgtgg
    12481 aaggctctcg cgagcgtgca cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc
    12541 ttatctggaa aatgactcag ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta
    12601 ccatgttgtt caacatccat accctggact gggacgacaa aatgctggaa gtgctggata
    12661 ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga agtatacggt cagactaaca
    12721 ttggcggcaa aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggccg
    12781 cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat ggcactggct
    12841 gctttatgct gatgaacact ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca
    12901 ccatcgcctg cggcccgact ggcgaagtga actatgcgtt ggaaggtgcg gtgtttatgg
    12961 caggcgcatc cattcagtgg ctgcgcgatg aaatgaagtt gattaacgac gcctacgatt
    13021 ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggcattta
    13081 ccgggctggg tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc ggtctgactc
    13141 gtggggtgaa cgctaaccac attatacgcg cgacgctgga gtctattgct tatcagacgc
    13201 gtgacgtgct ggaagcgatg caggccgact ctggtatccg tctgcacgcc ctgcgcgtgg
    13261 atggtggcgc agtagcaaac aatttcctga tgcagttcca gtccgatatt ctcggcaccc
    13321 gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg tgcggcctat ctcgcaggcc
    13381 tggcggttgg cttctggcag aacctcgacg agctgcaaga gaaagcggtg attgagcgcg
    13441 agttccgtcc aggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag
    13501 cggttaaacg cgcgatggcg tgggaagaac acgacgaata atgtaaatgc cgaatgaagc
    13561 gtttatgccg catccggtag tcccgaaacg tgcgggggca accccgcaca catcaataat
    13621 ccctcccttc ccctgtgcta cacttcgcgc cattccttac tgcttagagt ttgctatgag
    13681 acgagaactt gccatcgaat tttcccgcgt caccgaatca gcggcgctgg ctggctacaa
    13741 atggttagga cgcggcgata aaaacaccgc ggacggcgcg gcggtaaacg ccatgcgtat
    13801 tatgctcaac caggtcaaca ttgacggcac catcgtcatt ggtgaaggtg aaatcgacga
    13861 agcaccgatg ctctacattg gtgaaaaagt cggtactggt cgcggcgacg cggtagatat
    13921 tgctgttgat ccgattgaag gcacgcgcat gacggcgatg ggccaggcta acgcgctggc
    13981 ggtgctggca gtaggcgata aaggctgctt cctcaatgcg ccggatatgt atatggagaa
    14041 gctgattgtc gggccgggag ccaaaggcac cattgatctg aacctgccgc tggcggataa
    14101 cctgcgcaat gtagcggcgg cgctcggcaa accgttgagc gaactgacgg taacgattct
    14161 ggctaaacca cgccacgatg ccgttatcgc tgaaatgcag caactcggcg tacgcgtatt
    14221 tgctattccg gacggcgacg ttgcggcctc aattctcacc tgtatgccag acagcgaagt
    14281 tgacgtgctg tacggtattg gtggcgcgcc ggaaggcgta gtttctgcgg cggtgatccg
    14341 cgcattagat ggcgacatga acggtcgtct gctggcgcgt catgacgtca aaggcgacaa
    14401 cgaagagaat cgtcgcattg gcgagcagga gctggcacgc tgcaaagcga tgggcatcga
    14461 agccggtaaa gtattgcgcc tgggcgatat ggcgcgcagc gataacgtca tcttctctgc
    14521 caccggtatt accaaaggcg atctgctgga aggcattagc cgcaaaggca atatcgcgac
    14581 taccgaaacg ctgctgatcc gcggcaagtc acgcaccatt cgccgcattc agtccatcca
    14641 ctatctggat cgcaaagacc cggaaatgca ggtgcacatc ctctgattga tttgatcgat
    14701 tgagccttcc agtccttcgg gactggaatt tttttgttcg gagaacgaag ataaggcaag
    14761 tcaatcaaaa caggagaaaa acatggctga ttgggtaaca ggcaaagtca ctaaagtgca
    14821 gaactggacc gacgccctgt ttagtctcac cgttcacgcc cccgtgcttc cgtttaccgc
    14881 cgggcaattt accaagcttg gccttgaaat cgacggcgaa cgcgtccagc gcgcctactc
    14941 ctatgtaaac tcgcccgata atcccgatct ggagttttac ctggtcaccg tccccgatgg
    15001 caaattaagc ccacgactgg cggcactgaa accaggcgat gaagtgcagg tggttagcga
    15061 agcggcagga ttctttgtgc tcgatgaagt gccgcactgc gaaacgctat ggatgctggc
    15121 aaccggtaca gcgattggcc cttatttatc gattctgcaa ctaggtaaag atttagatcg
    15181 cttcaaaaat ctggtcctgg tgcacgccgc acgttatgcc gccgacttaa gctatttgcc
    15241 actgatgcag gaactggaaa aacgctacga aggaaaactg cgcattcaga cggtggtcag
    15301 tcgggaaacg gcagcggggt cgctcaccgg acggataccg gcattaattg aaagtgggga
    15361 actggaaagc acgattggcc tgccgatgaa taaagaaacc agccatgtga tgctgtgcgg
    15421 caatccacag atggtgcgcg atacacaaca gttgctgaaa gagacccggc agatgacgaa
    15481 acatttacgt cgccgaccgg gccatatgac agcggagcat tactggtaag cggttactta
    15541 tcgataaacg gcacgatgag caaatccgca ctcatcttat tgatcatccc gcgatatgcc
    15601 ggcatcaaac ggttgataaa tgagtgatga tgaccacaga caaggaggtc gcactgctct
    15661 ttttgcataa tttccagcag tgtttccggc atttctccgc gttcaatacg cagttttgtc
    15721 ttcggccatt gaatattttt cgtcagttta tacagcttgt tatccgactt attcttcaac
    15781 aattgaagaa tatcttctgt tgcagggaag tagatacccg gatacaactc gcttaagcca
    15841 tcatcaatat gaattaacgt caggtgagcg tcattatgtc tggcgagctc cagggcttta
    15901 ttcaccagta aggcatcttc ttcattcccg gaaattgcca cgccaatgtg tttataagcc
    15961 atgtttaact ccttctaaag caactccatc aagctaacga acgcagtgat aactcaaaaa
    16021 taatcatctc ggcctggcat gagaaagtga aggccgcatc aagctcaact tgcccctcca
    16081 gatccttaat cttataggcc acgctggccc cttcatctgc agaggatgta gcggcggcaa
    16141 gctctttcag tttggtcata aaagactctg cttcttcgcg ggtggcaaaa aagcgcgaaa
    16201 acttactggt acagttgtcg ttatcaatca ccgtaccgat atcaatcgca catcctttag
    16261 tactgcattt atctacgaca tctttcatag ggcacctctg tatattcgcc tctctgtcga
    16321 ttacgactta agaataatgc gccgttaagg gaagaacaaa caagtaatga tcttatggat
    16381 taccattttt tttgatattg cttaaggtga agagttcaga aggattttcc gggtattgtc
    16441 atttgcatcg tggaaataac aatcccggaa aaaaatagca gtaagagata tattaaaggt
    16501 taattacttg atttattgtc ggctttatac ttcacatcct gagtatcttt accatattta
    16561 ttttcgcctt gtgtgccaac aaacgcacca agatcgataa gcatcatcac cagaatcaac
    16621 gtcgggacaa aacgccccac cgcccattgc cagacacccg gtaaaatcgc ccagttaccc
    16681 gccagcagca tccacgccac aatcatcaga aatgcccatg cgccggaacg cccgcgatca
    16741 tgcaagcgct taacagttac tgccgctgtt ggccagagca agcacacaag gcaaaacgcc
    16801 gcggtctgaa tatcgagtag attcttaccc gccagtgaga aaagcaccag catgcctgcg
    16861 aaccacaggc ctatccaaat ccagaaatca cggcgtccaa tacgcccttt aaatgagaat
    16921 aaccattgct gtatggtcat gtaagttcct tgatggttgt cttttccagg attctaccct
    16981 tttgacaagg gcgacagata tcgttttaat cggagccagt cataacaaaa ggtactgtca
    17041 atgaagccag ggtgtacgct gttttttctc ttatgttctg cattaaccgt tacaacagcg
    17101 gcgcatgcac aaacaccaga tacggcaacg accgcgcctt atctgctggc tggagcccct
    17161 actttcgatc tctccatcag ccagtttcga gaagacttta acagccagaa tcccagcctg
    17221 ccactgaacg aatttcgtgc catcgacagc agtcccgaca aagccaatct cactcgtgct
    17281 gcaagtaaaa ttaatgagaa cttgtatgct tctacagcgc tggagcgcgg taccttaaaa
    17341 atcaaaagca ttcaaatgac ctggctaccc atccaggggc cagagcaaaa agccgcgaaa
    17401 gcgaaagctc aggaatacat ggcagcggtg atccgcacac tcaccccatt aatgaccaaa
    17461 acacaaagcc agaaaaaact gcagtcgcta ctaacggcgg ggaaaaacaa acgttattac
    17521 accgagacag aaggtgcact gcgttatgtt gtcgcggaca acggcgaaaa ggggctgacc
    17581 ttcgctgttg aaccgattaa gctggcgcta tctgaatcgc ttgaaggttt gaataaatga
    17641 caaaaagcaa agcctttgtg ccgatgaatc tctatactgt ttcacagacc tgctgccctg
    17701 cggggcggcc atcttccttt attcgcttat aagcgtggag aattaaaatg cgacatcctt
    17761 tagtgatggg taactggaaa ctgaacggca gccgccacat ggttcacgag ctggtttcta
    17821 acctgcgtaa agagctggca ggtgttgctg gctgtgcggt tgcaatcgca ccaccggaaa
    17881 tgtatatcga tatggcgaag cgcgaagctg aaggcagcca catcatgctg ggtgcgcaaa
    17941 acgtggacct gaacctgtcc ggcgcattca ccggtgaaac ctctgctgct atgctgaaag
    18001 acatcggcgc acagtacatc atcatcggtc actctgaacg tcgtacttac cacaaagaat
    18061 ctgacgaact gatcgcgaaa aaattcgcgg tgctgaaaga gcagggcctg actccggttc
    18121 tgtgcatcgg tgaaaccgaa gctgaaaatg aagcgggcaa aactgaagaa gtttgcgcac
    18181 gtcagatcga cgcggtactg aaaactcagg gtgctgcggc attcgaaggt gcggttatcg
    18241 cttacgaacc tgtatgggca atcggtactg gcaaatctgc aactccggct caggcacagg
    18301 ctgttcacaa attcatccgt gaccacatcg ctaaagttga cgctaacatc gctgaacaag
    18361 tgatcattca gtacggcggc tctgtaaacg cgtctaacgc tgcagaactg tttgctcagc
    18421 cggatatcga cggcgcgctg gttggtggtg cttctctgaa agctgacgcc ttcgcagtaa
    18481 tcgttaaagc tgcagaagcg gctaaacagg cttaagtctg acaggtgccg gatttcatat
    18541 ccggcactta ctttccttaa ctcttcgcct taacgcaaaa tctcacactg atgatcctga
    18601 atttcctcgg ctgaagcacg gttaagcgtc agtagatttc gttgtgtcgc cagcaataca
    18661 aatgagttat cactctgccg taccatcgcc agcccgtagc gtcccatatg ttcccgcgcc
    18721 tcaggtactt cttctgccag catcataaat gggctgcgtt gtaccagttc gctttccgtt
    18781 acccgacgcg ccaggtattc atgcccgcgc aaaccacctg gcagtggcaa ccagcggctg
    18841 ctgatgttcg ccagattgtt atccagctgt tcgcgcacat caggacgaat acaagagata
    18901 tgaatatgaa aatggttttg cgtacgcccg gtgcgggagt tgatcgccaa agaaaccgcg
    18961 cgatcgggaa ccggctggcc gtattttttg ctcataaaat cacgcgcctg ccaagccaac
    19021 caaaagaagt tcggcgttga aggatcggtc aacaaaggac tttcagtacc gttaatacga
    19081 tacgttggca tcaacagata ttgcagtggg ccattaagat cttttaaaac cacgtatccg
    19141 gcattgggtt tgacttccgc acatggcgaa ggattttgat tttgctgctg attgggcaaa
    19201 cattcctcaa ggacaatctt acgtaatgta tccgactctt caccggttaa tttccagtaa
    19261 ccaataccgg cagccacaac ggcgataact atcatcacca aaaaaagaag acccgctttt
    19321 ttcatctttt tttccctgta cctcaaagag atgcaagggt aacgcaaaat cgtgacaaat
    19381 aaaaaacccg gtcgcgagaa accaccgggt ttgataatta tcctggggaa atcagcgttt
    19441 gctgatctga tcgaacgtac cgccgttagc aaaatgctct ttttgcgctt tcgtccagcc
    19501 gccgaactct tcatcaatgg tgaataactt cagctttgga aacgcatttt cgtacttttt
    19561 cgccacctca gcgtcgcgcg gacggtagta gtttttcgcg gcaatttcct gaccttctgg
    19621 cgagtagaga tatttcaggt aggcttccgc cacctcttta gtaccttttt tctcgaccac
    19681 tttatcgacc accgacacgg ttggctctgc gaggatagac tcactcggcg tgacgatttc
    19741 gaatttatct ttccccagtt cattcgctgc cagcagagct tcgttttccc aggcaatcag
    19801 tacatcgcca attccgcgct cgacaaaagt gttagtggag ccacgcgcgc cagaatccag
    19861 aacttcgacg tttttataca gtgcccgaac aaaatcctgt gcttttgcct gatcgttgtt
    19921 gttgtgatgc agcgcgtagc cccaggctgc caggtagttc cagcgcgcgc caccagagct
    19981 tttcggatta ggcgtgatca ccgaaacacc cggtttaatc agatcgttcc agtcatggat
    20041 ctgcttcgga tttcccttac gtaccaggaa aacaatggtg gaagtgtacg gtgcggagtt
    20101 atccggcaga cgtttgatcc actctttatc aatccgcccg cgttccgcaa ttgcgtccac
    20161 gtcataggcc agagccagcg tgacaacatc agcttcaata ccgttgatta ccgacgtcgc
    20221 ttgtttacct gagccaccgt gtgactgacg aatcaccacg ttatcaccag tttgctgttt
    20281 ccagtgggcg ctgaatgcct tgttgtactg ttcgtacaat tcgcgcgttg gatcatatga
    20341 aacgttaaga agctgaatat cctttgccat aacgctggtt gccgccagca aaaatgttaa
    20401 ccctacgccc cacttgttca tcgcccgact ctcttatgtt gtgttgtgat gagcaaagcg
    20461 tgccagaagg ttaaccaaac attaaagaat aaaaaaagat tggctataac ttgcgggtat
    20521 atgttggggg attaaaaagg cggaagaaat ccgcctcata ttgctgacaa agtgcgcttt
    20581 gtccatgccg gatgcggcgc gaacgcctta tccggcctac aaaagtttgc aaattcaata
    20641 aattgcagaa ttcatgtagg cctgataagc gaagcgcatc aggcattttt gcttctgtca
    20701 tcggtttcag gctaaaggaa tctgcctttt tccgaaatca ttaatacagt tttttcgcgc
    20761 agtccagcca gtcacctttg aacggacgct tcatgttttc gatagcgtcg atgatgtcgt
    20821 ggtgaaccag ctgttcgttc tggataccta cacaacgacc gccgtaacct gccagcagca
    20881 gatcgatagc gtaagcgccc atacgggaag ccagaatacg gtcgtaaggc accggagaac
    20941 caccgcgctg gatgtggccc agcacagttg cgcgggtttc acgaccggtt tctttctcga
    21001 tgaaatgcgc cagttcgtca acatcacaca tatgttcggt aatcgccacg atcgcgtgtt
    21061 ttttaccttt cgcgataccc gctttgattt cgtttaccag gtcttcacgg ctgaattcaa
    21121 cttccggaac cacaacgaat tcacagccac cggcaatggc cgcagccagg gtcagatcgc
    21181 cacaataacg gcccatcact tccaccacgg aaatacgctg gtgagaagaa gaggtgtcac
    21241 gcagacggtc gatcgcttct acaacggtgc tcagcgcagt gaagaaaccg atagtgtagt
    21301 cagtgccttt gatgtcgttg tcgatagtgc ccggcaggcc gatgcacggg aagcccattt
    21361 cggtcagacg cattgcaccc atgtaggaac cgtcaccgcc gataaccacc agcgcgtcga
    21421 tcccacgttt tttcaggttt tcgatagcca cggcgcggat gttctcgtcg cggaattccg
    21481 ggaaacgcgc agaaccgagg aacgtaccgc cacggttgat catgtcagac acgctgtaac
    21541 ggtctagctg taccatacgg tcttcataca gacccagata gccgtcataa atgcccatta
    21601 cttccagacc ttctgtcagc gcagaacgaa caaccccgcg aattgcggcg ttcatgcctg
    21661 gcgcatcacc gccgcttgtc aacacaccga ttttcttaat catgactacc tctgaacttt
    21721 ggaatgcaaa ataaaatctg ttgccggaag tcttcttgca catcgaagtg atccaacgaa
    21781 tgtgcaaata gtataacaat cacttcctgc tgaattgatt caggtcaggc caaatggcgg
    21841 tattttatac acaaaatgcg ggtctggctc tcttttatac tgattatgaa agcatagacc
    21901 gtttaccctc cctgggtacg acggaacagg ggtcctgatg gataattaca tccgatcccg
    21961 gaaaacgccg taaaatagcc tgctctacct gatccgccac catatgtgcc tgaaccaaag
    22021 gcagagagtc ttccatttcc aaatgaatct gaataaagcg ggtcggccct gactgccgcg
    22081 tgcgaagatc gtgagcgccg ctaacacccg gccaggaagt cacgatatca ataatttctt
    22141 gccgttcctc atcaggcaat gcgcgatcca gtaatgactg taccgcctca tatcccatgc
    22201 gtaacgcgct atataaaata tagatgccga ttcccaatgc aaacagagca tcggcgcgat
    22261 gccagccgta ccaggacaac cccagcgcca gcagaattgc gccgttcatc ataacatcag
    22321 actggtaatg tagcatatca gcccgcaccg cctggctttg cgtccgccgc accacccaac
    22381 gctgaaacga gacaaggata atcgtacaaa ttagcgccac aattgtcacg ataaccccga
    22441 cgcctggatc tgtcatcggt gttggagata tcagatgttg aatacccgtc aaaaacagga
    22501 atagtgccga accggagata aacatacttt gcgccagcgc cgcgagggac tctgctttac
    22561 cgtgaccaaa cgagtgatta tcgtcggcag gttgcaggga atatcgcacc accagtaaat
    22621 tcgtcaacga cgcgccgata tccaccagcg aatccaccag cgcggcgaga atactcaccg
    22681 acccggtata ccaccatgca aaaattttaa tcagcaatag caacgaagcc atcgccgtcg
    22741 cagcaatcgc cgcccgactg accagccgtc cataagattg attcataaat actcccgcta
    22801 tcaactgacg ctagtataac ggaagcaaat catctgcaat gcattaagca gcaggcaaat
    22861 tgaggataaa aaaaaccccc acatcatgtg ggggaagaca gggatggtgt ctatggcaag
    22921 gaaaacaggg tttactactg ggaacgtgag ttgctactac tcaatagctt caacgatgaa
    22981 cttttttgcc attgcgtcac gtcgcgcaac tgctccattc gttgttgatg tttctcgttt
    23041 aaaaccgctt gctgctccgg cgttaacagg cgatacattt ggttgcggac ttttgccatc
    23101 tcaacctgac gagcaatttg ctcattcgcc attttttctg cctgtgcgcg cacagcgttt
    23161 tcatcaaaat tttctgcggt gacaaggcga tgcattgtct ccagttcgct aacattaaca
    23221 ggaggctgtt cgtgccgggc ctgttgcata agatctcgca tctgctgacg ctgatgttcg
    23281 gttaaactta tgccgtcgaa catatggctc tgcgtactgc gctgcgtaag ttcttcaccc
    23341 ggatgccagt tatcgcctga accgacttca gcagcgtggc ttaatgaact gactgccagc
    23401 gttgaggcca tgacggcagc ggtaactatg cgcatcattt gctcccaaaa tctttctgtc
    23461 gcgattcaac gatagagagt ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa
    23521 caacgtaaag tcatggatta gcgacgtctg atgacgtaat ttctgcctcg gaggtattta
    23581 aacaatgaat aaaatcctgt tagttgatga tgaccgagag ctgacttccc tattaaagga
    23641 gctgctcgag atggaaggct tcaacgtgat tgttgcccac gatggggaac aggcgcttga
    23701 tcttctggac gacagcattg atttactttt gcttgacgta atgatgccga agaaaaatgg
    23761 tatcgacaca ttaaaagcac ttcgccagac acaccagacg cctgtcatta tgttgacggc
    23821 gcgcggcagt gaacttgatc gcgttctcgg ccttgagctg ggcgcagatg actatctccc
    23881 gaaaccgttt aatgatcgtg agctggtggc acgtattcgc gcgatcctgc gccgttcgca
    23941 ctggagcgag caacagcaaa acaacgacaa cggttcaccg acactggaag ttgatgcctt
    24001 agtgctgaat ccaggccgtc aggaagccag cttcgacggg caaacgctgg agttaaccgg
    24061 tactgagttt accctgctct atttgctggc acagcatctg ggtcaggtgg tttcccgtga
    24121 acatttaagc caggaagtgc tgggcaaacg cctgacgcct ttcgaccgcg ctatcgatat
    24181 gcacatttcc aacctgcgtc gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa
    24241 aaccttgcgt ggtcgcggct acctgatggt ttctgcttca tgataggcag cttaaccgcg
    24301 cgcatcttcg ccatcttctg gctgacgctg gcgctggtgt tgatgttggt tttgatgtta
    24361 cccaagctcg attcacgcca gatgaccgag cttctggata gcgaacagcg tcaggggctg
    24421 atgattgagc agcatgttga agcggagctg gcgaacgatc cgcccaacga tttaatgtgg
    24481 tggcggcgtc tgttccgggc gattgataag tgggcaccgc caggacagcg tttgttattg
    24541 gtgaccaccg aaggccgcgt gatcggcgct gaacgcagcg aaatgcagat cattcgtaac
    24601 tttattggtc aggccgataa cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa
    24661 ctggtcggtc cgttctccgt acgtgatggc gaagataatt accaacttta tctgattcgt
    24721 ccggccagca gttctcaatc cgatttcatt aacttactgt ttgaccgccc gctattactg
    24781 ctgattgtca ccatgttggt cagtacgccg ctgctgttgt ggttggcctg gagtctggca
    24841 aaaccggcgc gtaagctgaa aaacgctgcc gatgaagttg cccagggaaa cttacgccag
    24901 cacccggaac tggaagcggg gccacaggaa ttccttgccg caggtgccag ttttaaccag
    24961 atggtcaccg cgctggagcg catgatgacc tctcagcagc gtctgctttc tgatatctct
    25021 cacgagctgc gcaccccgct gacgcgtctg caactgggta cggcgttact gcgccgtcgt
    25081 agcggtgaaa gcaaggaact ggagcgtatt gaaaccgaag cgcaacgtct ggacagcatg
    25141 atcaacgatc tgttggtgat gtcacgtaat cagcagaaaa acgcgctggt aagcgagaca
    25201 ttgaaagcta accagttgtg gggcgaagtg ctggataacg cggcgttcga agcagaacag
    25261 atgggcaagt cgttttctgt taacttcccg cctgggccgt ggccgctgta cggcaatccg
    25321 aacgccctgg aaagtgcgct ggaaaacatt gttcgtaatg ctctgcgtta ttcccatacg
    25381 aagattgaag tgggctttgc ggtagataaa gacggtatca ccattacggt ggacgacgat
    25441 ggtcctggcg ttagcccgga agatcgcgaa cagattttcc gtccgttcta tcggaccgat
    25501 gaagcacgcg atcgtgaatc tggcggtaca ggattggggc tggcgattgt tgaaaccgcc
    25561 attcagcagc atcgtggctg ggtgaaggca gaagacagcc cgctgggcgg tttacggctg
    25621 gtgatttggt tgccgctgta taagcggagt taaactccgc atttgtaggc aggataaggc
    25681 gtttacgccg catccggcat ttgagcagga tgcctgatgc gacgctgata gcgtcttatc
    25741 aggcctacac tccgggggtt attttcccca caaccgccgg gaaaaatctt cgatcttgcc
    25801 gctcagtcgg cgcttttgca tcgttcgcgt ccagcttttc gataacccgg cagcggagag
    25861 taaacggtga tactgatcgt catcaaacgg catatgccat gcgatggcgg cagcctcctg
    25921 cacggtcaca tcgctgacac gggaaaccaa ctccagcggc gcgtccgcag acactttccc
    25981 tggcgctatc acgctataca accatcccac tttacccgtg ttttgcatca actgcgctat
    26041 atcgctgata tcaaaatggt aattgagttt atagcaaggc gagcgcggct ggctgacctg
    26101 aattaatgcc tctccccagc ggaaaatatc gcccatataa acattacttt ccgtcaggcc
    26161 gtcggttgag aggttttcac caaacgcagg cgcaacaaac aactccgcct gttccggaaa
    26221 ttcccgcgcc cagtagagat aatgctcacg aggataatga cacagcgctc tgtctggccc
    26281 gccgtgaacc tttttctccg cctgctcgtc accttccagc cccagctccg tcagcatcaa
    26341 ctcaccatca acctggattt tagcaattgc gctgggtttg ccttcgggat aagcctgaat
    26401 cttgcctgtg tatacatcaa ccggatatcg catctgttcc ctctccacga gaaatttccc
    26461 cattagacca tatttatttt atgtcaggcg tgctgttttc gcggtaagta tccaggcagg
    26521 aactcaatag caaaccggac gaacgttagt tcatccggct gtggggatta aggaagcagg
    26581 tttatttcac cgtacctaaa atttcgattt tcgctgctct ggctttgact ttacgtgacc
    26641 aaatagaggt aagaattggc accagaatcg aagtcacaat gaccgccgtc gctaccagcg
    26701 aagttgctgc cggagccatc ggtttaaacg caggcaccat ttctgcaatc agcacaggtg
    26761 tcgctaccgc ggcccctgcg gaactggaag cggcaattcc ggcagtgcca tcgccaccgc
    26821 caatcaattt atcggcgata atcaacggaa taccggtcac gataattact gccacaccca
    26881 acaggatccc cagcaaacca gtctgggcaa tcacagtcaa atcaatggta ttgcccagcg
    26941 cgaaggcaaa gaatggaatc agcgtttgca ccgctttgct gaaaaattct cgtaattcag
    27001 ggtcaaggtt ccccagggca aagcccacca ggaacggtaa tactgcgccg acgaaaacat
    27061 gcggttcaaa cgaggcaatc ccggcagtgc ccagaataat catcgtcatg agcggcccgg
    27121 actccaacga catcaacaca aatgccccag cttcttcttt cgtaccgtac tgctgcatga
    27181 tggaagcgta aagtccgccg ttggtcatat ccatcgccgc caccagcgcc agcgttgaaa
    27241 gtccggcaaa gaatccaact tcaacaccat gttccggaat aatacgcgag gcaattgccg
    27301 caaccaccca cgcgacggca attttagtta ccaccagcgt accggattta cgcagtaccg
    27361 ttcccgtcgc gcttaatttt attgacgccc ccatgcaaaa aaaccacacc gccagaatgg
    27421 gcaccgtacc ggtaatcatc ccgttggtga atgctccaaa atatttcccc gcccccggcg
    27481 agaaggtgtg gcacagtgcg ccaaggaata gcgggacgag catcatcccc cccgggattt
    27541 tctcaatcga gcgttttatc tgcatttcca tcacctgtcg gcatattttt aagggctgat
    27601 gcgtaattcc tcaatggagc atcagtgttg atggaaacga agatagacat tcatttaatc
    27661 aataaaagtg atcaaaacca catcaattga aacactgttt tatttttatc ggatcattgt
    27721 ttttattttg aaattgatca caaaaaaacc accgcagttg gcgatggttc attttttaag
    27781 ctgatatgcg gcctatacgc ctcattgcag caggcggcaa atgattattt tttcgccgca
    27841 aaacgtgccg ctgcttcgtc ccagttcacc acgttccaga actctttaat gtagtccgga
    27901 cggcggttct ggaatttcag gtagtaagca tgttcccaca catccaggcc cagaatcggg
    27961 aagccggaag cgccagaaat agcttcaccc atcagcggag aatcctggtt agcagtagaa
    28021 accaccgcca gtttatcgcc tttcagcacc agccatgccc agccggaacc aaagcgggaa
    28081 gctgccgctt tttcaaattc tgctttgaag ttatcaacgg agccgaagtc acgttcgata
    28141 gccgctttca ggtcaccctg cagggtggtg ccttttttca gacctttcca gaacaggctg
    28201 tggttagcgt gaccgccagc gttgttgcgc agtacggttt tcttgtctgc tggcagctgg
    28261 tccagtttgg tgatcagctc ttcaaccggc aggttggcaa attctggcag gctttccagc
    28321 gccgcgttgg cgttgtttac gtaggtctga tggtgtttgg tgtggtggat ttccatggtc
    28381 tgcttatcga agtgcggttc cagggcatcg taagcatacg gcagggatgg cagggtatag
    28441 ctcatattca tctccagtat tgtcgggcgg ccgattgtta atgccgcgta agcagttggt
    28501 tcattatagt taattaaatg atattgaaaa tgattatcaa tgccgtactt ttcgtaaggg
    28561 tatggttttg caggaaaatg cccgagatgt gaagcaaatc acccacttaa tgccgtgatt
    28621 gccagtaaat cgacaacggc ggcaacaggc gaaaggttaa tcgacagcac gatttttaca
    28681 ctcatctcgt cggagatgtg acgcgacgaa aaatgatgag gataagaaga tgagtaacgc
    28741 gattacgatg gggatatttt ggcatttgat cggcgcggcc agtgcagcct gtttttacgc
    28801 tccgttcaaa aaagtaaaaa aatggtcatg ggaaaccatg tggtcagtcg gtgggattgt
    28861 ttcgtggatt attctgccgt gggccatcag cgccctgtta ctaccgaatt tctgggcgta
    28921 ttacagctcg tttagtctct ctacgctact gcctgttttt ctgttcggcg ctatgtgggg
    28981 gatcggtaat atcaactacg gcctgaccat gcgttatctc ggcatgtcga tgggaattgg
    29041 catcgccatt ggcattacgt tgattgtcgg tacgctgatg acgccaatta tcaacggcaa
    29101 tttcgatgtg ttgattagca ccgaaggcgg acgcatgacg ttgctcggcg ttctggtggc
    29161 gctgattggc gtagggattg taactcgcgc cgggcagttg aaagagcgca agatgggcat
    29221 taaagccgaa gagttcaatc tgaaaaaagg gctggtgctg gcggtgatgt gcggcatttt
    29281 ctctgccggg atgtcctttg cgatgaacgc cgcaaaaccg atgcatgaag ccgctgccgc
    29341 acttggcgtc gatccactgt atgtcgctct gccaagctat gttgtcatca tgggcggcgg
    29401 cgcgatcatt aacctcggtt tctgttttat tcgtctggca aaagtgaagg atttgtcgct
    29461 aaaagccgac ttctcgctgg caaaatcgct gatcattcac aatgtgttac tctcgacact
    29521 gggcgggttg atgtggtatc tgcaattctt tttctatgcc tggggccacg cccgcattcc
    29581 ggcgcagtat gactacatca gttggatgct gcatatgagt ttctatgtat tgtgcggcgg
    29641 tatcgtcggg ctggtgctga aagagtggaa caatgcagga cgccgtccgg taacggtgtt
    29701 gagcctcggt tgtgtggtga ttattgtcgc cgctaacatc gtcggcatcg gcatggcgaa
    29761 ttaatctttc tgcgaattga gatgacgcca ctggctgggc gtcatcccgg tttcccgggt
    29821 aaacaccacc gaaaaatagt tactatcttc aaagccacat tcggtcgaaa tatcactgat
    29881 taacaggcgg ctatgctgga gaagatattg cgcatgacac actctgacct gtcgcagata
    29941 ttgattgatg gtcattccag tctgctggcg aaattgctga cgcaaaacgc gctcactgca
    30001 cgatgcctca tcacaaaatt tatccagcgc aaagggactt ttcaggctag ccgccagccg
    30061 ggtaatcagc ttatccagca acgtttcgct ggatgttggc ggcaacgaat cactggtgta
    30121 acgatggcga ttcagcaaca tcaccaactg cccgaacagc aactcagcca tttcgttagc
    30181 aaacggcaca tgctgactac tttcatgctc aagctgaccg ataacctgcc gcgcctgcgc
    30241 catccccatg ctacctaagc gccagtgtgg ttgccctgcg ctggcgttaa atcccggaat
    30301 cgccccctgc cagtcaagat tcagcttcag acgctccggg caataaataa tattctgcaa
    30361 aaccagatcg ttaacggaag cgtaggagtg tttatcgtca gcatgaatgt aaaagagatc
    30421 gccacgggta atgcgataag ggcgatcgtt gagtacatgc aggccattac cgcgccagac
    30481 aatcaccagc tcacaaaaat catgtgtatg ttcagcaaag acatcttgcg gataacggtc
    30541 agccacagcg actgcctgct ggtcgctggc aaaaaaatca tctttgagaa gttttaactg
    30601 atgcgccacc gtggctacct cggccagaga acgaagttga ttattcgcaa tatggcgtac
    30661 aaatacgttg agaagattcg cgttattgca gaaagccatc ccgtccctgg cgaatatcac
    30721 gcggtgacca gttaaactct cggcgaaaaa gcgtcgaaaa gtggttactg tcgctgaatc
    30781 cacagcgata ggcgatgtca gtaacgctgg cctcgctgtg gcgtagcaga tgtcgggctt
    30841 tcatcagtcg caggcggttc aggtatcgct gaggcgtcag tcccgtttgc tgcttaagct
    30901 gccgatgtag cgtacgcagt gaaagagaaa attgatccgc cacggcatcc caattcacct
    30961 catcggcaaa atggtcctcc agccaggcca gaagcaagtt gagacgtgat gcgctgtttt
    31021 ccaggttctc ctgcaaactg cttttacgca gcaagagcag taattgcata aacaagatct
    31081 cgcgactggc ggtcgagggt aaatcatttt ccccttcctg ctgttccatc tgtgcaacca
    31141 gctgtcgcac ctgctgcaat acgctgtggt taacgcgcca gtgagacgga tactgcccat
    31201 ccagctcttg tggcagcaac tgattcagcc cggcgagaaa ctgaaatcga tccggcgagc
    31261 gatacagcac attggtcaga cacagattat cggtatgttc atacagatgc cgatcatgat
    31321 cgcgtacgaa acagaccgtg ccaccggtga tggtataggg ctgcccatta aacacatgaa
    31381 tacccgtgcc atgttcgaca atcacaattt catgaaaatc atgatgatgt tcaggaaaat
    31441 ccgcctgcgg gagccggggt tctatcgcca cggacgcgtt accagacgga aaaaaatcca
    31501 cactatgtaa tacggtcata ctggcctcct gatgtcgtca acacggcgaa atagtaatca
    31561 cgaggtcagg ttcttacctt aaattttcga cggaaaacca cgtaaaaaac gtcgattttt
    31621 caagatacag cgtgaatttt caggaaatgc ggtgagcatc acatcaccac aattcagcaa
    31681 attgtgaaca tcatcacgtt catctttccc tggttgccaa tggcccattt tcctgtcagt
    31741 aacgagaagg tcgcgaattc aggcgctttt tagactggtc gtaatgaaat tcagcaggat
    31801 cacattatga cctttcgcaa ttgtgtcgcc gtcgatctcg gcgcatccag tgggcgcgtg
    31861 atgctggcgc gttacgagcg tgaatgccgc agcctgacgc tgcgcgaaat ccatcgtttt
    31921 aacaatgggc tgcatagtca gaacggctat gtcacctggg atgtggatag ccttgaaagt
    31981 gccattcgcc ttggattaaa caaggtgtgc gaggaaggga ttcgtatcga tagcattggg
    32041 attgatacct ggggcgtgga ctttgtgctg ctcgaccaac agggtcagcg tgtgggcctg
    32101 cccgttgctt atcgcgatag ccgcaccaat ggcctaatgg cgcaggcaca acaacaactc
    32161 ggcaaacgcg atatttatca acgtagcggc atccagtttc tgcccttcaa tacgctttat
    32221 cagttgcgtg cgctgacgga gcaacaacct gaacttattc cacacattgc tcacgctctg
    32281 ctgatgccgg attacttcag ttatcgcctg accggcaaga tgaactggga atataccaac
    32341 gccacgacca cgcaactggt caatatcaat agcgacgact gggacgagtc gctactggcg
    32401 tggagcgggg ccaacaaagc ctggtttggt cgcccgacgc atccgggtaa tgtcataggt
    32461 cactggattt gcccgcaggg taatgagatt ccagtggtcg ccgttgccag ccatgatacc
    32521 gccagcgcgg ttatcgcctc gccgttaaac ggctcacgtg ctgcttatct ctcttctggc
    32581 acctggtcat tgatgggctt cgaaagccag acgccattta ccaatgacac ggcactggca
    32641 gccaacatca ccaatgaagg cggggcggaa ggtcgctatc gggtgctgaa aaatattatg
    32701 ggcttatggc tgcttcagcg agtgcttcag gagcagcaaa tcaacgatct tccggcgctt
    32761 atctccgcga cacaggcact tccggcttgc cgcttcatta tcaatcccaa tgacgatcgc
    32821 tttattaatc ctgagacgat gtgcagcgaa attcaggctg cgtgtcggga aacggcgcaa
    32881 ccgatcccgg aaagtgatgc tgaactggcg cgctgcattt tcgacagtct ggcgctgctg
    32941 tatgccgatg tgttgcatga gctggcgcag ctgcgcggtg aagatttctc gcaactgcat
    33001 attgtcggcg gaggctgcca gaacacgctg ctcaaccagc tatgcgccga tgcctgcggt
    33061 attcgggtga tcgccgggcc tgttgaagcc tcgacgctcg gcaatatcgg catccagtta
    33121 atgacgctgg atgaactcaa caatgtggat gatttccgtc aggtcgtcag caccaccgcg
    33181 aatctgacca cctttacccc taatcctgac agtgaaattg cccactatgt ggcgcagatt
    33241 cactctacac gacagacaaa ggagctttgc gcatgaccac tcaactggaa caggcctggg
    33301 agctagcgaa acagcgtttc gcggcggtgg ggattgatgt cgaggaggcg ctgcgccaac
    33361 ttgatcgttt acccgtttca atgcactgct ggcagggcga tgatgtttcc ggttttgaaa
    33421 acccggaagg ttcgctgacc ggggggattc aggccacagg caattatccg ggcaaagcgc
    33481 gtaatgccag tgagctacgt gccgatctgg aacaggctat gcggctgatt ccggggccga
    33541 aacggcttaa tttacatgcc atctatctgg aatcagatac gccagtctcg cgcgaccaga
    33601 tcaaaccaga gcacttcaaa aactgggttg aatgggcgaa agccaatcag ctcggtctgg
    33661 attttaaccc ctcctgcttt tcgcatccgc taagcgccga tggctttacg ctttcccatg
    33721 ccgacgacag cattcgccag ttctggattg atcactgcaa agccagccgt cgcgtttcgg
    33781 cctattttgg cgagcaactc ggcacaccat cggtgatgaa catctggatc ccggatggta
    33841 tgaaagatat caccgttgac cgtctcgccc cgcgtcagcg tctgctggca gcactggatg
    33901 aggtgatcag cgagaagcta aaccctgcgc accatatcga cgccgttgag agcaaattgt
    33961 ttggcattgg cgcagagagc tacacggttg gctccaatga gttttacatg gggtatgcca
    34021 ccagccgcca gactgcgctg tgcctggacg ccgggcactt ccacccgact gaagtgattt
    34081 ccgacaagat ttccgccgcc atgctgtatg tgccgcagtt gctgctgcac gtcagccgtc
    34141 cggttcgctg ggacagcgat cacgtagtgc tgctggatga tgaaacccag gcaattgcca
    34201 gtgagattgt gcgtcacgat ctgtttgacc gggtgcatat cggccttgac ttcttcgatg
    34261 cctctatcaa ccgcattgcc gcgtgggtca ttggtacacg caatatgaaa aaagccctgc
    34321 tgcgtgcgtt gctggaacct accgctgagc tgcgcaagct ggaagcggcg ggcgattaca
    34381 ctgcgcgtct ggcactgctg gaagagcaga aatcgttgcc gtggcaggcg gtctgggaaa
    34441 tgtattgcca acgtcacgat acgccagcag gtagcgaatg gctggagagc gtgcgggctt
    34501 atgagaaaga aattttgagt cgccgcgggt aaacactgcc ggatgcggcg cgagcgcctt
    34561 atccggccta cgggtcggca acagttgtag gcctgataag acgcgacagc gtcgcatcag
    34621 gcattgattg ccggatgcgg cacaagtgcc ttatccggcc tacaggtcgg caatagttgt
    34681 aggcctgata agacgcgaca gcgtcgcatc aggcattgat tgccggatgc ggcacaagtg
    34741 ccttatccgg cctacaggtc ggcaacagtt gtaggcctga taagacgcga cagcgtcgca
    34801 tcaggcattg attgccggat gcggcacaag tgccttatca ggcctacagg tcggcaatag
    34861 ttgtaggcct gataagacgc gacagcgtcg catcaggcat tgattgccgg atgcggcgcg
    34921 agcgccttat ccggcctacg ggtcgtgcat ccgacaacac cgaatttaca ggaacacaga
    34981 acatgcaaaa cattactcag tcctggtttg tccagggaat gatcaaagcc accaccgacg
    35041 cctggctgaa aggctgggat gagcgcaacg gcggcaacct gacgctacgc ctggatgaag
    35101 ccgatatcgc accatatcac gacaatttcc accaacaacc gcgctatatc ccgctcagcc
    35161 agcccatgcc tttactggca aatacaccgt ttattgtcac cggcttgggc aaattcttcc
    35221 gtaacgtcca gcttgatcct gcggctaact taggcatcgt aaaagtcgac agcgacggcg
    35281 cgggctacca cattctttgg gggttaacca acgaagccgt ccccacttcc gaacttccgg
    35341 ctcacttcct ttcccactgc gagcgcatta aagccaccaa cggcaaagat cgggtgatca
    35401 tgcactgcca cgccaccaac ctgatcgccc tcacctatgt acttgaaaac gacaccgcgg
    35461 tcttcactcg ccaactgtgg gaaggcagca ccgagtgtct ggtggtattc ccggatggcg
    35521 ttggcatttt gccgtggatg gtgcccggca cggacgaaat cggccaggcg accgcacaag
    35581 agatgcaaaa acattcgctg gtgttgtggc ccttccacgg cgtcttcggc agcggaccga
    35641 cgctggatga aaccttcggt ttaatcgaca ccgcagaaaa atcagcacaa gtattagtga
    35701 aggtttattc gatgggcggc atgaaacaga ccatcagccg tgaagagttg atagcgctcg
    35761 gcaagcgttt cggcgttacg ccactcgcca gtgcgctggc gctgtaagga gcaaacatga
    35821 tccgcaaagc ctttgtcatg caggtaaacc ccgacgccca cgaagagtat cagcgtcggc
    35881 ataatcccat ctggccagaa ctggaagcag tgctgaaatc tcacggtgcg cataactacg
    35941 ccatctatct cgacaaagcg cgtaatctgc tgtttgccat ggtagagatt gaatctgaag
    36001 aacgctggaa tgcggttgcc agtactgatg tttgccaacg ttggtggaaa tacatgaccg
    36061 atgttatgcc cgctaacccg gataacagcc cggtgagtag cgagctgcaa gaagtgtttt
    36121 acctgcctta atgagttgcg gtgccggaaa atatccagca ccgcgccttc atcagaagaa
    36181 gtatttaaag cgaacgcgtg cgccgtaacg gtcatcagta tcatcaccgt gttttttatc
    36241 accgtcgcta tccagaatgg agtaatacgt cccgaggtaa atattaaagt tctccatatc
    36301 catcacatta gcgaactgat aagacgcatg aatggtgtga atggagtacg tcccttcatc
    36361 atcaatccag cagtcgttat cacaaactac accgctgaat tcatcaatct tattgtgcgc
    36421 gtagatataa ccgagctcga aacgtttcca cagcgcgtta atcccggcag tgaaatcttt
    36481 ctcattatta gcatctaaat aggcggtatt aagattaacc acaatgccat tgcccggatc
    36541 cgttttcagg ccgttccagg tcatgctcat cccatagccg gtacgatcgg actgatcgac
    36601 aaaattaccc ttgctatcgg tataaccata agcattattt accacgttcg cttccatcgc
    36661 tgcggaaacg gtgaattctt ccgtcggcga ccaggcaatt actgggcgca gataagcaac
    36721 atttttctgc tgctccatat cgcgtccatg ataattaccg tcattatata aagatgttcc
    36781 gtcttcaagt aacgtattta attcaaaata ccagttatcg agttgtttgc tgacgaggaa
    36841 attaccgcca gcgttagagc gcccgcggcc ctctttcatc atatagatat aaccgctgcc
    36901 atcgtcataa agatcgttcg cagtattacc ggaatgttca acaaaggtat cctgattcag
    36961 cgggaacata tcgtaggctt caaaacggcc tactttgacc ttccagtcgt tttctttacc
    37021 aaagaagaat accgcatcat cgatatttac tgaaccgtgc atatcaccca gcggttgtgc
    37081 ggagaacccg gcgaaataac cgttgtccat ctttcgcata ccgtcaaaac ctaacagaat
    37141 acgaccattg agattccact gttcattagt gggatcgcta ttcacatcag cgttggtcat
    37201 tgccagcagg ccatgattac tttccgcatc catattaaat tctacgtcgc cgtagatttt
    37261 taaatcgcca taacctgaaa gcaaaagatt aggtgaggtg gcatttttct ttgcagattc
    37321 cgccgttgcc tgtccattta ccggcgtatt gccctgagcg gccttaattt cgcggatctc
    37381 agcggcttgt tgtgtctgca gctgtttaac ctggctctct gcttttgctg cccgcatttc
    37441 tgccgcttcc aggcgttctt ccagcaatgc catgcgttgt tccagcgtta aatgctgtgt
    37501 ttttgccata ccggtactac atgtcaataa taaaccgatg gctaaagata atatcgattt
    37561 tttcattatt tacgttccct gaaataaagg agaccctacg ctttccaaaa ggaatacgta
    37621 tatagagaaa tataaagtgt gcactttcga atttttaatc caacttcagc aggccgatgg
    37681 atagtatccc gcggcataat atgagtaaat ccgctgattt cctggaacgt gaggaatata
    37741 acaatagtgc cgtttttaac taaatatcgt aaaaaatatt agatatgatt caaactgact
    37801 cataagccac aaatataaat aacgtggcag caaaatatcc gcgacaatta aacattcaca
    37861 aaatcattaa ataatgattt tttattcaca acattt
//
LOCUS       DAJOSY010000033        36705 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJOSY010000033 DAJOSY010000000
VERSION     DAJOSY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.033"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            142..1326
                     /locus_tag="OHN27_001782"
     CDS             142..1326
                     /locus_tag="OHN27_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCQ2286234.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1437..2360
                     /locus_tag="OHN27_001783"
     CDS             1437..2360
                     /locus_tag="OHN27_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2286235.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLSVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(2364..3182)
                     /gene="nlpA"
                     /locus_tag="OHN27_001784"
     CDS             complement(2364..3182)
                     /gene="nlpA"
                     /locus_tag="OHN27_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCQ2286236.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            3404..3697
                     /locus_tag="OHN27_001785"
     CDS             3404..3697
                     /locus_tag="OHN27_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286237.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(3738..4928)
                     /gene="nepI"
                     /locus_tag="OHN27_001786"
     CDS             complement(3738..4928)
                     /gene="nepI"
                     /locus_tag="OHN27_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCQ2286238.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(5139..5591)
                     /locus_tag="OHN27_001787"
     CDS             complement(5139..5591)
                     /locus_tag="OHN27_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCQ2286239.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5644..6978)
                     /gene="adeQ"
                     /locus_tag="OHN27_001788"
                     /gene_synonym="yicO"
     CDS             complement(5644..6978)
                     /gene="adeQ"
                     /locus_tag="OHN27_001788"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCQ2286240.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAIVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            7153..8919
                     /gene="adeD"
                     /locus_tag="OHN27_001789"
     CDS             7153..8919
                     /gene="adeD"
                     /locus_tag="OHN27_001789"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCQ2286241.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8964..10355)
                     /gene="uhpT"
                     /locus_tag="OHN27_001790"
     CDS             complement(8964..10355)
                     /gene="uhpT"
                     /locus_tag="OHN27_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCQ2286242.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10493..11812)
                     /gene="uhpC"
                     /locus_tag="OHN27_001791"
     CDS             complement(10493..11812)
                     /gene="uhpC"
                     /locus_tag="OHN27_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCQ2286243.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(11822..13324)
                     /gene="uhpB"
                     /locus_tag="OHN27_001792"
     CDS             complement(11822..13324)
                     /gene="uhpB"
                     /locus_tag="OHN27_001792"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCQ2286244.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(13324..13914)
                     /gene="uhpA"
                     /locus_tag="OHN27_001793"
     CDS             complement(13324..13914)
                     /gene="uhpA"
                     /locus_tag="OHN27_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCQ2286245.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13989..14279)
                     /gene="ilvN"
                     /locus_tag="OHN27_001794"
     CDS             complement(13989..14279)
                     /gene="ilvN"
                     /locus_tag="OHN27_001794"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCQ2286246.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(14283..15971)
                     /gene="ilvB"
                     /locus_tag="OHN27_001795"
     CDS             complement(14283..15971)
                     /gene="ilvB"
                     /locus_tag="OHN27_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCQ2286247.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(16077..16175)
                     /gene="ivbL"
                     /locus_tag="OHN27_001796"
     CDS             complement(16077..16175)
                     /gene="ivbL"
                     /locus_tag="OHN27_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCQ2286248.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            16740..16829
                     /gene="tisB"
                     /locus_tag="OHN27_001797"
     CDS             16740..16829
                     /gene="tisB"
                     /locus_tag="OHN27_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCQ2286249.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            17109..18293
                     /gene="emrD"
                     /locus_tag="OHN27_001798"
     CDS             17109..18293
                     /gene="emrD"
                     /locus_tag="OHN27_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCQ2286250.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANILLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(18301..18798)
                     /locus_tag="OHN27_001799"
     CDS             complement(18301..18798)
                     /locus_tag="OHN27_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCQ2286251.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(18795..19157)
                     /locus_tag="OHN27_001800"
     CDS             complement(18795..19157)
                     /locus_tag="OHN27_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCQ2286252.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(19147..19494)
                     /locus_tag="OHN27_001801"
     CDS             complement(19147..19494)
                     /locus_tag="OHN27_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCQ2286253.1"
                     /translation="MNISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(19665..20873)
                     /locus_tag="OHN27_001802"
     CDS             complement(19665..20873)
                     /locus_tag="OHN27_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2286254.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKGLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSLPGRNVRAKSGLNRSILDQGWYEIRRQLAYKQLWRGGQVLAVPPAYTSQRC
                     VCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            20943..21065
                     /locus_tag="OHN27_001803"
     CDS             20943..21065
                     /locus_tag="OHN27_001803"
                     /inference="COORDINATES: protein motif:HMM:NF013921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2286255.1"
                     /translation="MHVHLVFVTRYRRQIFDYDATEKLRTYFSNVCADFEAELV"
     gene            21066..21311
                     /gene="tnpA"
                     /locus_tag="OHN27_001804"
     CDS             21066..21311
                     /gene="tnpA"
                     /locus_tag="OHN27_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCQ2286256.1"
                     /translation="MDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAV
                     RYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG"
     gene            complement(21442..22764)
                     /locus_tag="OHN27_001805"
                     /pseudo
     CDS             complement(21442..22764)
                     /locus_tag="OHN27_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(22764..24380)
                     /locus_tag="OHN27_001806"
     CDS             complement(22764..24380)
                     /locus_tag="OHN27_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCQ2286257.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            24676..25392
                     /locus_tag="OHN27_001807"
     CDS             24676..25392
                     /locus_tag="OHN27_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCQ2286258.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(25389..27050)
                     /locus_tag="OHN27_001808"
     CDS             complement(25389..27050)
                     /locus_tag="OHN27_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCQ2286259.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(27246..27674)
                     /gene="ibpB"
                     /locus_tag="OHN27_001809"
     CDS             complement(27246..27674)
                     /gene="ibpB"
                     /locus_tag="OHN27_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCQ2286260.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(27786..28199)
                     /gene="ibpA"
                     /locus_tag="OHN27_001810"
     CDS             complement(27786..28199)
                     /gene="ibpA"
                     /locus_tag="OHN27_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCQ2286261.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(28193..28265)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            28505..28837
                     /locus_tag="OHN27_001811"
     CDS             28505..28837
                     /locus_tag="OHN27_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCQ2286262.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(28839..30053)
                     /locus_tag="OHN27_001812"
     CDS             complement(28839..30053)
                     /locus_tag="OHN27_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCQ2286263.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTEHHANSPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            30154..31218
                     /gene="cbrA"
                     /locus_tag="OHN27_001813"
     CDS             30154..31218
                     /gene="cbrA"
                     /locus_tag="OHN27_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCQ2286264.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKTVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(31215..32552)
                     /locus_tag="OHN27_001814"
     CDS             complement(31215..32552)
                     /locus_tag="OHN27_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2286265.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(32627..33775)
                     /gene="dgoD"
                     /locus_tag="OHN27_001815"
     CDS             complement(32627..33775)
                     /gene="dgoD"
                     /locus_tag="OHN27_001815"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HCQ2286266.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGPIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(33772..34389)
                     /gene="dgoA"
                     /locus_tag="OHN27_001816"
     CDS             complement(33772..34389)
                     /gene="dgoA"
                     /locus_tag="OHN27_001816"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCQ2286267.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(34373..35251)
                     /gene="dgoK"
                     /locus_tag="OHN27_001817"
     CDS             complement(34373..35251)
                     /gene="dgoK"
                     /locus_tag="OHN27_001817"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCQ2286268.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQSDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREEKTPVVMAGMVGSNVGWKVAPYLSVPACFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(35248..35937)
                     /gene="dgoR"
                     /locus_tag="OHN27_001818"
     CDS             complement(35248..35937)
                     /gene="dgoR"
                     /locus_tag="OHN27_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCQ2286269.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            36215..>36705
                     /locus_tag="OHN27_001819"
     CDS             36215..>36705
                     /locus_tag="OHN27_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286270.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYS"
BASE COUNT         9164 a         9834 c         9183 g         8524 t
ORIGIN      
        1 ctcaattgct ggccctataa tattgcgcta gcattgcttt ctggttgtat cagcgatact
       61 caaaaattct taatacaata ctcattcgac tggtacttat ttgtaactca gttatatttt
      121 ttcgcccggt gattcagaag aatgcaaaaa acggctacca ctccatcaaa aatacttgat
      181 ctcactgccg cggcattttt acttgtcgcc tttctgacgg gtattgcggg cgctcttcag
      241 actcctaccc taagtatatt cctcgcagat gaactgaaag cccgtcctat aatggtaggt
      301 tttttcttca ccggtagcgc tattatggga attctggtca gtcaatttct ggcaaggcac
      361 tccgataaac aaggcgaccg taaattactg attctgctat gttgcttatt tggagtgctg
      421 gcctgcacgc tttttgcgtg gaatcgcaac tacttcattc tcctctcaac gggcgtactt
      481 ctgagtagtt ttgcttccac cgcaaacccg caaatgttcg ccctcgcccg tgaacacgcc
      541 gacagaacag gccgtgagac ggtcatgttc agtacatttt tacgtgctca gatctcgctt
      601 gcctgggtta tcgggccacc gctcgcttat gaactggcaa tgggatttag ttttaaagtg
      661 atgtatctca ccgctgccat cgcatttgtt gtttgcgggc tgatagtctg gttgtttttg
      721 ccatcaatac aaagaaatat tcctgtcgtt acccaacccg tagaaatttt accctccacc
      781 cacaggaagc gggatacgcg gctacttttt gtggtctgtt caatgatgtg ggcggcgaat
      841 aatctctaca tgataaatat gccgctattt attattgatg aactgcatct aaccgataaa
      901 ctggctggag aaatgattgg tatcgctgcc ggtctggaaa ttccgatgat gttaatcgca
      961 ggctattaca tgaaacgtat tggcaagcga ctattaatgc tcattgctat cgtgagtgga
     1021 atgtgttttt acgccagcgt actcatggcg acgactccgg cggttgagct ggaattgcaa
     1081 attcttaatg ccatcttcct tggtattctc tgtggtatcg gcatgcttta ttttcaggac
     1141 ctgatgcctg aaaaaatagg ctctgcgaca acgttatatg caaatacttc acgcgtcggc
     1201 tggattatcg ccggctctgt tgacggaatt atggttgaaa tctggagcta ccatgcgttg
     1261 ttctggctgg cgatagggat gttgggtatt gcgatgattt gcctgctgtt tattaaagat
     1321 atttagctca ccagtctggg ctaagaaaaa tcggcgtggc gcaaacagag ccatcctcgc
     1381 catttttgct atcatgcctg catacataaa cgacaaaaca gtatgcagag ggaaaaatgg
     1441 gttccaccag aaaggggatg ctgaacgttc tgattgccgc cgtgttgtgg ggaagttcag
     1501 gggtctgcgc gcaatacatc atggagcaaa gccagatgtc gtcgcagttt ttgactatga
     1561 cgcgtttgat attcgccggt ttgattctac tgacgctgtc atttgttcat ggcgataaaa
     1621 tcttttctat tattaacaat cataaagatg caattagcct gctgattttt tccgtggttg
     1681 gcgcgctaac tgtacagctc acttttttgc taaccatcga aaaatcgaac gcagccacgg
     1741 caacggtgct gcaattcctc tcaccgacga ttatcgtcgc ctggttctca ctggtgcgta
     1801 aatcgcgccc gggcattctg gttttctgcg ctattttgac atcgctggtc gggacttttt
     1861 tattggtgac acacggtaat ccgacgtcat tatcgatctc tcctgccgcg ttgttctggg
     1921 gcattgcctc ggcatttgct gctgcattct ataccaccta tccctcaacg ctaattgccc
     1981 gctatggcac gttgtctgtc gtcggctgga gtatgctgat cggcggtctg attctgttgc
     2041 ctttttatgc cagacaaggg acaaactttg tcgttaacgg cagtttgatt ttggcgtttt
     2101 tttatttgat cgtcattggt acgtccctga catttagtct gtacctgaaa ggagcacaat
     2161 taattggcgg tccaaaagcc agcattttga gctgtgcaga accattaagt agcgcgctac
     2221 tctctttgct gttgctgggg atcacgttca cattaccgga ctggctggga acgctgctga
     2281 ttctgtcatc ggtaattttg atttcaatgg attcccgtcg ccgcgccaga aaaataaatc
     2341 gtccggcgcg ggatgagtga gaattaccag ccaggcaccg cgccaccgtt aaagatggtt
     2401 tctgccgctt tagcgacttc ggatgattga taagattgca gaaattcctt cacgttttct
     2461 gcattcttat tatcttcccg tgccaccaaa atattcacat acggcgaatt tttatcttca
     2521 ataaatacgc tgtcgtgcac cggagaaagc ccggtctgct gaatgtaagt ggtgctgata
     2581 atcgccacat caactttggg atcatccagt acgcgcggga gctgcgcccc ctcgagttcc
     2641 ataatctgca aatgacgagg attatcggtt atatccagcg cggtaggtaa taaccctttt
     2701 ccctctttca gagtaattag cttctctttt tgcaacagta aaagtgcgcg accaaggttg
     2761 gtcgggtcgt tggggatcgc cactgtcgcg ccttctttta tttgcgccac ggttttgatt
     2821 tttttggaat aacccgccat agggaagaca aacgtatttc ccaccgctac cagtttataa
     2881 ccatgcgcct gattatcttg ttcaagaaag ggtcgatgct ggaagacatt cgcatcaagt
     2941 tcgccatgat ttgtcgcatc gttcggtagc aacgaaccgc taaatcctac cagttcaaca
     3001 tcgaggccat atttctcttt tgccaccttt ttagcgactt ccgcgacatc ttgttctgcg
     3061 ccatttataa cgccaacttt aatgtgtttt gcatcgctgc tactctggtc gcaacctgcc
     3121 agcagaattc cggccagcaa taatgcggcc cctgtccgta gatgatgtgt tgtcagtttc
     3181 atttttttat ccttttatta atgattacct gactatagcg gtaaggtcgc tgcggtttaa
     3241 aaaacgaaac gctatcgata agaataaaaa ggaataaaag tggaatataa agtctggcac
     3301 ttcttactga ccacgcaagc gcgtttcgta cagcatgatg agagcgatga aagcaaacta
     3361 cacttatgct tcattcgata cacatttgta aaaggttgaa tccatgaaac caacgacact
     3421 actgcttatt ttcacttttt tcgctatgcc aggcatcgta tacgccgagt cgccttttag
     3481 ctcactgcag tccgcaaaag agaaaaccac ggtattacaa gatttacgta aaatttgcac
     3541 gccacaggcg tcattatcag atgaagcgtg ggaaaagtta atgttgtcag atgaaaacaa
     3601 taaacaacat atacgcgagg ccatcgtggc gatggaacgt aataatcaga gcaattattg
     3661 ggaagcgttg ggtaaggtag aatgcccgga tatgtaagaa cagcaacaac tactgaccaa
     3721 cgcttcttta cctgatatca ggatttcttc attttcacct ttgcagtaac caacaatgca
     3781 gtcagcaaca tcaatgtgcc ggacaacatc aacggcgaag tcagaccaat attatccagc
     3841 gcataaccgc cgattgccgc gccacaggta ttagcaagct gaataaccgc cacctgaata
     3901 gacccggctt tttctgcctg atcggccagc gagcgggtga tccacgttga ccagccgacg
     3961 ggaaccaatg caaaagttag cccccagata atcgccacgc cggtagcaac gattttatcg
     4021 cttccccaca acgtcagtac caacgcactc acagccagta ttaacggcgc gcctgctaag
     4081 gccagtttta ccgaacgttt aagaatgaac gacgaaagcg acgtaccaat aaagctggcg
     4141 ataccaaaac tcaacagcac cagcgttaag ccatccacgc cgaatcccgc caggttcata
     4201 tacaccgggc gaatatacgt gaagaaagca aactgcccgg cgaaagacat gaagatggcg
     4261 atcatccctg ccatcacacc cggacgttgt aataagcgga aagtattttg tttctgatgc
     4321 gagggttcgc ctggcagtga aggcaatgat ttgataatcc agaaaataca cagcacgccc
     4381 atcaccgccg ccgcattaaa gacattgcgc caaccgataa gctcgcctaa aaaactgccc
     4441 aacggcgcgg caatcaccag cgcaatagaa accgcgccga agatcaccga cagcgccttc
     4501 ggcaccgtac gcggcggcac cagacgcatg gtcagcgacg ccgacatcgc ccagaacccg
     4561 cccagcgcca gccccagaca ggcacgaccg attaaaagca aactgaatga gttagcaaag
     4621 gaaaccagca agcaggagag cgtcagcaaa acggcaaaca aaataacaac gtagcggcgg
     4681 tcagtagcct gaattgtctg ggtaataaac aaactggcaa acattgccac aaaggcggtc
     4741 acggtcaccg attgcccggc aaccccttcc gaaatgccta aatcctgggc cattggcgtc
     4801 aacaaactga cgggcaaaaa ctcaacgata atcagacagg cgacacaaaa cgccaccgag
     4861 aaaacggctg accagttcgg tcgggtgatg gcatccgcgc cgcggttttc ggcaataaat
     4921 tcactcatgg tgttacccgt ggcatgtttc aggggaaaag ccgaccactt taacattgaa
     4981 atgtgtgacg catttaacgt ttttgcaact tttacggtgt tgcttgagct ggagttaaga
     5041 tcgaaacgga acagggcaaa gattaccgga tgcggcgtga acgtcttatc cacccgacag
     5101 gtcaggcgcg gtctgtaggc tgataagacg aaacggtttc aatgcaacca gtgaacgccc
     5161 tcggcaggct gaaagaacgc gttatagcgc atctgaaaag cattaatatc ctgcatttca
     5221 ggttcggttt cgcgcagaaa acctagcgcc agcctgacct gagcgtcggt aatcggcgcg
     5281 gcgaggcgag ctttttgtaa aatccgatgc cattgcagca ggttctgttc gctaccatcg
     5341 acaatgacaa tttgccagat gagcaacacc tgcgcggcgt tttgcaggtg cgactcatcc
     5401 ggacgatgca aataacgtaa aaattcgtca ccagctgact ttcccatttg atcgaccatc
     5461 gactccacgg gtacgacatc gatgttcagc cgatcgctga gacggcgaat cgcttttctg
     5521 gccccggatg tcagcacccg aaaaccaacc acaaacacta ccgccagcgt ggcgagcatt
     5581 atccagatca tgagttctcc tgcgtaatca gaactcatga tatctggaaa ccctcgccag
     5641 ttactaatcc accagaataa tcttcagtgc aaacagagct gcgaccacca ccacacacag
     5701 gttcagatcg cgccagcgcc cggtgcatac tttcatgatg cagtacgaca taaagccgag
     5761 tgcaatccct tcggtgatcg agaaagtaaa gggcatcatc accgtggtaa taaacgcagg
     5821 caccgattcg gtaaaatcat cccagttaac gcgcgccagg ctcgaagtca tcagcacgcc
     5881 aacaaagatt aacgctccgg cggttgcgta aggaggaact atcgccacca gcggtgagaa
     5941 gaacataacc aacaggaaca taacgccaac cacaaccgca gtcagccccg tgcggccacc
     6001 gactgccaca ccagaagtac tttcaatata ggcagtaaca gacgaggtgc cgataaacgc
     6061 acccgccacc gaactgacgc tatcaacata cagcgcctta ttcatattgg ggaatttacc
     6121 gttaccatct attaagcccg ctttatcagt tacaccaatt aatgttcctg atgaatcaaa
     6181 taggttgatc agcataaagg agaaaatgat accggcgagt tcaagtgtta acgcgccgct
     6241 caaatctact tcaccaatga cgccgctaat atcaggcgga atggaataga cgccgctaaa
     6301 atgaacatca ccgaaaaata atccacagca agacgtcacc acaatagaaa ccagcaccgc
     6361 ggcatgaaaa tgacgtgatg acaacacggt tataataaaa aaccctaaaa tacctaacaa
     6421 cacgccgtga gaacttaaat cgccaatcat caccagcgtg tctttattgg cgacaataac
     6481 gccagtattt tttaatccca ttaaggcgat aaataatcca attccgctgg tgataccaat
     6541 acgtaaactt aagggaatgt tggagatcat ccagtaccgg atacgaaaca gcgtgagcaa
     6601 aaatagtcca actgcgcccc agaatattgc gcccatcccg gtctgccagg agatgcccat
     6661 cgcccccacg accacgaagg caaagaaggc gttcagcccc attgccggag ccagcgccac
     6721 gggtaagtta gcaaatatcc ccatcgcaat actgccgata ccggcaatca aacaggtggt
     6781 aacaaacacc actttcgggt ccatttgtgc cgcgccgagg atttgcgggt tcacaaaaac
     6841 gatgtacacc atggttaaaa aagtggtcat ccccgcaatc aattctgtgc ggacggtggt
     6901 cccatgctga ggtagtttaa ataatcgcga aagcgtccct gattcattac tcacgtaatc
     6961 ggtattgtca ttattcattt ttttgtccat tggaggagat ttaatccctt gcttttgtaa
     7021 aacaaatgac aacaacggtt cagtgataat tatcacattt caattgcaca ttaatggata
     7081 ttctttaata atctcgcgac gtttctttat gataaataat aatcaaattg ataaaatcaa
     7141 aatgagaaaa atatgaataa ttctattaac cataaatttc atcacattag ccgggctgaa
     7201 taccaggaat tgttagccgt ttcccgtggc gacgctgttg ccgattatat tattgataat
     7261 gtctctattc tcgacctgat caatggcgga gaaatttccg gcccaattgt gattaaagga
     7321 cgttacattg ccggtgttgg cgcagaatac gctgatgctc cggctttgca gcggattgat
     7381 gctcgcggcg caacggcggt gccagggttt attgatgctc acctgcatat tgaatccagc
     7441 atgatgacgc cggtcacttt tgaaaccgct accctgccgc gcggcctgac gaccgttatt
     7501 tgcgaccctc atgaaatcgt caacgtgatg ggagaagccg gattcgcctg gtttgcccgc
     7561 tgtgccgaac aggcaaggca aaaccagtac ttacaggtca gctcttgcgt acccgccctg
     7621 gaaggctgcg atgttaacgg tgccagtttt acccttgaac agatgctcgc ctggcgggac
     7681 catccgcagg ttaccggcct tgcagaaatg atggactacc ctggcgtaat tagcgggcag
     7741 aatgcgctgc tcgataaact ggatgcattt cgccacctga cgctggacgg tcactgcccg
     7801 ggtttgggtg gtaaagaact taacgcctat attactgcgg gtattgaaaa ctgccacgaa
     7861 agttatcagc tggaagaagg acgccggaaa ttacaactcg gcatgtcgtt gatgatccgc
     7921 gaagggtccg ctgcccgcaa tctcaacgcg ctggcaccgt tgatcaacga atttaacagc
     7981 ccgcaatgca tgctctgtac cgatgaccgt aacccgtggg agatcgccca tgaaggacac
     8041 atcgatgcct taattcgccg cctgatcgaa caacacaatg tgccgctgca tgtggcatat
     8101 cgcgtcgcca gctggtcgac ggcgcgccac tttggtctga atcacctcgg cttactggca
     8161 cccggcaagc aggccgatat cgtcctgttg agcgatgcgc gtaaggtcac ggtgcagcag
     8221 gtactggtga aaggcgagcc gattgatgcg caaaccttac aggcggaaga gtcggcgaga
     8281 ctggcacaat ccgctccgcc atatggcaac accattgccc gccagccagt ttccgccagc
     8341 gactttgccc tgcaatttac gcccggaaaa cgctatcggg tcattgacgt catccataac
     8401 gaattgatta cgcactccca ctccagcgtc tacagcgaaa atggttttga tcgcgatgat
     8461 gtgagcttta ttgccgtact tgagcgttac gggcaacggc tggctccggc ttgtggtttg
     8521 cttggcggct ttggactgaa tgaaggtgcg ctggctgcga cggtcagcca tgacagccat
     8581 aatattgtgg tgatcggtcg cagtgccgaa gagatggcgc tggcggtcaa tcaggtgatt
     8641 caggatggcg gcgggctgtg cgtggtacgt aacggccagg tccaaagtca tctgccgttg
     8701 cccattgccg gcctgatgag caccgacacg gcgcagtcgc tggcggaaca gattgacgcc
     8761 ttgaaagccg ccgcccgtga atgcggtccg ttacccgatg agccgtttat tcagatggcg
     8821 tttctttctc tgccagtgat ccccgcgcta aaactaacca gtcaggggct atttgatggc
     8881 gagaagtttg ccttcactac gctggaagtc acggaataat aaaaaagccc ggcgtcatgc
     8941 cgggcaaaag tcaccagtta cgtttatgcc actgtcaact gctgaatttt tttctcgcgg
     9001 cggattttgc gttcttccat caccgccact atcgccatca ggcagataca accaatcgcg
     9061 gcgatatcaa gtgcggcgaa ggtgcctgcc cagccggtaa ggccgaatac cggcgtccca
     9121 tcggcaatca ttcccagacc taacttggca aagctgtcac caatcaggta agcaaaggtg
     9181 cctttaatac catcggcagc gccaatcgct tttttaggta caaagccaac agcagccaca
     9241 ccaatcaaca attgcgggcc aaagaccagg aaacccaacg caaagagaga agccagatag
     9301 atatattcgt tgctggcatg ttgatacaca ccgagcgtgg cgataatcag cgccagcgcg
     9361 atgcaggcca ccaggccacg gcgaccgttc gccaggtcag agagccagcc ccacagcagc
     9421 gtaccgacca gcgcaccagc ttcaaacagc gtaaagccct gaatcgccac cgctttagag
     9481 agtttcagtt cctggaacgc gtatacggtt gaccactggt cgataccaat acgtaccaca
     9541 tagaggaaaa tgttggcgaa gcacagcagc cagatcactt tgtttttcag cacatactca
     9601 acaaagatct gccacttggt catatcggta gattctgtct ctttgtcctc ttcgctgatc
     9661 tcctcgccga acagttcttc agctttgccg aggccataag attccgggga gtcgctaccg
     9721 taacgcaggc cgataaaacc gacaatcagc gcgataatcg acgggaagat aaacatgccg
     9781 atgacatggc catcgaacag gtaatttgcc ccgaacagcg ccacacctgc tgcgcctgca
     9841 ccgccaaggt tgtgagaaat attccagaaa ccgaggaatg tcccgcgttt acgacgcggc
     9901 gtccatttgg tgatggtgga gtaactgcac gaaccgccgg tactctggaa aaagccgctt
     9961 aaggcgtaga aggcaatcat caggaacagg ctaaccgagc cgctgcccat actggcactg
    10021 aagcccagca tacaaatagc agagaggata agcatgaacg gcaggaattg tttggtgttt
    10081 ttgccgtcgg cgtagtatga aaccaacgtt ttacccacgc cataagtgat ggagaaaccc
    10141 aggccgatca tccccagctg cgtcatgctc aacccgtagg tcgaaatcat atcgttctgc
    10201 gcgatgttaa agttcttgcg aatcaggtac atcgtcaggt agccgataaa gaccaccagg
    10261 taggattgca tgaacggttt gaaccacatt ttgcgccgca cttcgagcgg aaggtccagg
    10321 gtcggcttgc gaacttggtt taggaaagcc agcatgggtt actcctgaaa tgaatacctg
    10381 cgtgaggcat gcattgtaaa aatcagcagc aaatcaggcc tgagatagcg tccaggaaaa
    10441 acctggaaaa tttcttagtt ttgccggata tgaagtgaaa aggtgagatg catcacgctt
    10501 cacgcggtgt ctgggcgttc aaaaagggca gtaacagcag tgcggaaatc ccggcggcga
    10561 tagagataac cacaaaaaat ccgctccagt gccaggtatc gagcactttc gccagcggcc
    10621 aaccagcaag cgacgccccc agataagcaa acaagccgac aaaccccgtc gccgcccctg
    10681 ccgcctcttt gtgggaacac tctgccgccg ccataccaat taacatctgc gggccaaaga
    10741 caaaaaaacc aatggtgaag aagcaggttg cctgcatcac gtagctggca aatggcatca
    10801 gccacaggga gccgactgaa agcaaaattc cggcggcgaa aatcaaattc atcggccctc
    10861 ggttgccgtt aaacaatttg tccgagcccc aacccgctac cagcgcaccg ataaatccac
    10921 ccagttcaaa catcgtcact gccgtattcg ccgtgaccag atcgacgccc agcgtctcgg
    10981 acatatacaa attgccccag tcgttgatcg ccgcccggac cacatagacc agcacatagc
    11041 aaaacgaaag cagccagata tacggattca gcaacacata tttggtgagg atctctttac
    11101 gcgtcagccc tgccccttct tgttgttgag ctatctccag cgcgtcgtgt cgccattcac
    11161 cgaccgccgg taaacctaac gcctgcgggc gatcgcgcag ccgccagcag agaaaaattc
    11221 ccacgacgat cgccatacaa ccagcaatca tcatcccggc acgccagccg taatgtagtg
    11281 cagccgctgc catcacaatg ggaatgagtg cgccgccgac gttatgcgcc gtgttccata
    11341 atgcccacca accgccgcgc tcggtacgag aataccaggc cgttaacaga cgcgcacaca
    11401 ccggtgaacc ccagccctgg aaaaaggcgt tcagcaccca gagcacggca aacgcccata
    11461 gcgacgtcga gaagccaaac agaatgttga tgatgccagt ggcgataagc cctatcccca
    11521 taaaataacg ggcatttgag cgatcgctga caatgccgga gacaaacttc gacacgccgt
    11581 aagtaatgta aaacagggtc gctaataggc cgatatcgct acggctgagc acgccgttag
    11641 caaggatttc tggtacggcg gcgttaaaac ttttacgcgt gaagtaaaac agcgcgtaac
    11701 ccagccagat ggtcagcaga atatgccgac gccagtagcg ataacgggca tcaatttcat
    11761 gtttatcagt cattaatggc gcatcggcag gcgctttcag aaacggcaac atcatcgaac
    11821 cttagacata acgttgaggt agagaaacgc tgacacgcgt gccgtgcaga caggaaatgt
    11881 gtaatgtgcc acccagcgcc gttacgcgct cgcgcattcc ggtgaggcca aaaccttgtt
    11941 gcccggaacc cggcggcaaa ccgctgccat cgtcttcaat aaccagcatc aaccgttcat
    12001 cctgctgcca gccttgcagg gtgaccgcgc tggcatcagc atgtttcaca atgttgttca
    12061 gcccttcctg gcagacacga aacagcgtca cgcgctggtt ttcgcttaac gctgattcat
    12121 cgattcgcca ttcgagatgg ctgacaatac cgcgcccttc cagctccatt tcccgcatca
    12181 gtgagcggat ggcctgctcc agggtgagat catccaactg gcgcggacgt aaccgaccca
    12241 acaaacggcg caccgcgtcg taaacgccca gcgatagttg ttcgatgagc tgcccgctct
    12301 gcttcacgct ggcgttatct gccgccagcc gctgaacaat gcccgcctga gtacgaatag
    12361 cagtgatggt ctgaccgata tcatcatgca gctcacgcgc cacatcacgg cgcacgctct
    12421 cttcggtttc cagcaaccgt tcagccagat gctgattgcg cgccagttcc ttttgcagcg
    12481 actggttaag ttcacgcaac cgctggatgc cagcgccaag caacaaccct gtcagacttt
    12541 gcaccagcag cgagagcaat aaatccaccg gatgatcgcg ccaggtttga ctggcgatca
    12601 gcgcgatggc gttcatcaac gtcgcaatca gcgccccttg ccaaccatag tgccaggcca
    12661 gcgcgataat cggcagcgcc agacagaatg gcgtaaagcg cgacagttcg tccggcaatc
    12721 ccaactggag ccagagactg ataacaaaca gcagcaagta ccagaccaga tgtcgcccgc
    12781 gccagttgat tggctgagaa accagtgacg ggccgagcgg cagccaggtg ttattggcga
    12841 gatagtgcca gaacaccaga catatcgggg ccagcgtcag gccgccagtt aaagtcagca
    12901 acagcgcatt ccacgactct ttgccgtgcc aaagccaggg cagcgactgc aacaacgccg
    12961 ccgccgttaa cgccgccccc tgtaacagca aggtgcgcca gtcacgctga tggcgatagc
    13021 gcgagatcag cgctacgggc agtaacgtca gtaaactacc gatcatcaat aacggaaaat
    13081 gggttaaacc gaccgcctgc gttagccagt aaatcagcag ccactccgcg cccagcagca
    13141 ccggccagta tccgcgcggg cattgcagca ttagccccag acgcagacca aacggaaata
    13201 acagcaccgc catatcaggg cgctcaacca gatgcaggct gatactccac aggcaaaacc
    13261 atgcggcaga gaagataaaa aagcaggcaa taacggtaat taagcgggag aacaacgtct
    13321 tcatcaccag ccatcaaaca tgcggcgcgc cagctctacg tcgttactga cgcccagttt
    13381 ttccatcaga ttggcgcgat ggacgtgtac cgttttcggt gacaagccca gttcggcggc
    13441 aatctctttc accgccattc cttgcgccag tttttccgcc acctggcgtt cacgtttggt
    13501 tagcgggtcc tgacgaccgg atgccagttt aatggcaata tccggcgtca gataacagcc
    13561 gcccgtggca accgtatgca ccgcagcaat gagttcatcc gggctacagc gtttggaaag
    13621 aaagccgcgt gcccccgcgt taagcgcctg ctcaaccagc gcaggactgt cgtgaacgga
    13681 gagcataatc gtcgccatac ctttcggcag ctggcttagc agctccagac cggagatatc
    13741 gggcatggag atatcgcaaa tacacacctg cacaccgcgc cccggcagcc ccgccagcgc
    13801 ctcgcgcccc gaaccaaact cggcaactac ctgcaaatca ggttccagcc ccagcagctg
    13861 cgcaaagccg gagcggacga tgaggtgatc gtctataagg gcaacggtga tcatggtctt
    13921 gtcctggcgg gtaaaaaaaa acgcgcttac cttaacgata agcgcgatgt tgttcaagcc
    13981 ttgagcggtt actgaaaaaa caccgcgatc ttgttaaaca tcgtcggatc ggactgatta
    14041 cgctgcactt tcacgacatc ttccagctta tcgatttggc ttatcatctg ctccagacgc
    14101 tggtcgtcat tgaccagtag ccagatatgg cttttgtcgc tgtcctgaat cggcagacaa
    14161 agaatgcctt caacgttaaa agcgcggcgg gcaaaaaggc cacaaacgtg ggtcattacg
    14221 cccggatggt tgcgaacggt gagctccaga attacgttgt catgagttgt gttttgcatg
    14281 gcttattccc ccaccatttc agtattcgcc gcacctggcg gcaccatcgg gtaaactttt
    14341 tcttcggcat caatgcgcac atggatcagc gccgggccag ggcgattgat gatttcctgc
    14401 aatgcagcct gcggatcggc ttcgttattc aaatcacagg tttcgaggcc gaatccggcg
    14461 gcaatctgca taaagttgat tttgcccgga taggtggcgg caaaaacgcc ttgctcgtag
    14521 aacagactct gttgctgatg caccagcccc agcgcttcgt tgttcatcag aatgattttg
    14581 acatccagct gattttcact ggcggtcgcc atctcctgaa tattcatcat caggctgccg
    14641 tcgccggaga aacacaacac tttgcgatcc gggttcgcca gcgccgcgcc aatcgccgca
    14701 ggcaggccaa aacccatcgt gcccagccca ccggaggtca gccactggcg tgggcgattg
    14761 agcggataag cttgcgcggt ccacatctga tgctgcccca catcggtggt gataatcgca
    14821 ttgtcatcga cacaggcggc aacggcgttg atcaggccgt aatggcttaa tggatcgcac
    14881 gctttcggga ttggacacgg aaactcacgc tgcaaatccg ctaccaactg gtgccactct
    14941 gcacgcggtt gcgcttccac cagcgggatc aactgcgcca gcacgtcatc aacatccgcc
    15001 tgaatcgcca catgcggctg cttgatttta cccagctctg cacggtcgat atcgacatga
    15061 atgattttgg cattcggaca gaactgctcg gttttgccaa tcgcccggtc atcaaaacgc
    15121 gcaccgagca caatcagtaa atccgcctcc tgcaagatat agttagtgct gcgcacgccg
    15181 tgcatcccca gcatacccag cgacaacgga tgcgcttttg gcagcatgcc cagcgccatt
    15241 aaagtcatgg tggtaggcag ttgcgctttc tccgccagtt cacgcacccg cgcaggcgca
    15301 ttgatcacac caccacccag ataaagcacc gggcgtttgg cagcgttaat cattgtagct
    15361 gcgtcacgaa tgctttcttc gctaaaggcg ggtgcagcgg ctttttctgc caccgcgggc
    15421 tgagcttcaa tctcaaaaac cgccgtttgc acatccttag gaatgtctat ccacaccggg
    15481 cctgggcggc ctgattgcgc aatgcggaag gcatcgctca tgacctgcgg gagttcttcg
    15541 atatgtctga ccagatagtt gtgtttggtg atggggatag agatgccgta ggtgtcgact
    15601 tcctggaagg cgtcggtgcc gatcatcgag gcgggaacct gaccagtgat gcaaatcagc
    15661 gggatggagt ccagccgcgc atcggcaatg gcggtcacca ggttagtcgc acccggtccg
    15721 ctacaggcca tacagaccgc cggtttaccg tcggtgcgcg ccattccctg agcgataaat
    15781 cccgcgccct gttcatggcg agccagaata tggcggattt gcgtactttg gcttaaggca
    15841 tcgtaaacag gcaggataga accgcccgga atgcccgtca caatcttaat gccctgctgt
    15901 tccaggaaat gaacgataaa ttctgcgccg gtaaagcgct tacgcgtcga tgttgtgccc
    15961 gaacttgcca tgctccagtc cttttcttct gggccgactt tccgggaggt gcttaaacga
    16021 aaaaccccgc ccggtttgcg ccggcggggt tttggaatcg tgtgttgttc cagtccctac
    16081 ggcgcattgc cgacgaccac caccacacgc acgacgacca ctgcggcgga tggcgcagtt
    16141 ggtagtagtt ttgcgttgaa catggaagta gtcattgggg accttgttgg ttttgtgttt
    16201 aacaatattt atacaagcac agctttacag gggagacaat ggaaaatttt tcagcaaggg
    16261 aaaattgagg ggttgatcac gttttgtact gaattgcaga taacaaaaaa ccccgccgga
    16321 gcgaggtttc gtcagtcgcc tgcggctggt aaccgcaaag cacactgtat tatgtcaaca
    16381 ctgaaagtat acgtgttccg cgcagaacgc gcaatttcgg cacgaatttt gacgtattta
    16441 gtgcatagtt gagtatcgat cacagtttgc gttttgtcca aatattactg tttatttata
    16501 cagtaaactt ctataatatc actgtacgca atgtgttatg cgggggccgc atcgttaccc
    16561 ggcgcactaa gtcctggctg aaacgggtgg tgccgtcagc gccttaaccc cgcgtgagca
    16621 cactgtgtta tgtcaacaag cacaacgttt ctccttgaga taccgcgtgc acaacagctg
    16681 gcaacaggca gcggaaaggt acgtcagctg gcagtgctcc tgaaccacag gagacgcgta
    16741 tgagcctggt ggatatcgcc attcttatcc tcaaactcat tgttgcagca ctgcaactgc
    16801 ttgatgctgt tctgaaatac ctgaagtaat tcagattcaa gtcgcaccaa aggggagcgg
    16861 gaaaccgctc cccttttata tttagcgtgc gggttggtgt cggatgcgat gctgacgcat
    16921 cttatccgcc ctaccatctc tcccggcaac atttattgcc gcttttgttt acatattctg
    16981 ccgctaaaca attccccatt cctggcgtat atctggctaa cattcatcaa tgtgatagat
    17041 tcctctcccg catttatggg aatgcgtagt gacttattct aattattttt ataaaagcat
    17101 ccgtgataat gaaaaggcaa agaaacgtca atttgttatt gatgttggta ttactcgtgg
    17161 ccgtcggtca gatggcgcaa accatttata ttccagctat tgccgatatg gcgcgcgatc
    17221 tcaacgtccg tgaaggggcg gtgcagagcg taatgggcgc ttatctgctg acttacggtg
    17281 tctcacagct gttttatggc ccgatttccg accgcgtggg ccgccgaccg gtgatcctcg
    17341 tcggaatgtc catttttatg ctggcaacgc tggtcgcggt cacgacctcc agtttgacgg
    17401 tgttgattgc cgccagcgcg atgcagggaa tgggcaccgg cgttggcggc gtaatggcgc
    17461 gtactttgcc gcgagattta tatgaacgga cacagttgcg ccatgctaac atcctgttaa
    17521 acatggggat tctcgtcagt ccgttgctcg caccgctaat cggcggtctg ctggatacga
    17581 tgtggaactg gcgcgcctgt tatctctttt tgttggttct ttgtgctggt gtgaccttca
    17641 gtatggcccg ctggatgccg gaaacgcgtc cggtcgatgc accgcgcacg cgcctgctta
    17701 ccagttataa aacgcttttc ggtaacagcg gttttaactg ttatttgctg atgctgattg
    17761 gcggtctggc cgggattgcc gcctttgaag cctgctccgg cgtgctgatg ggcgcggtgt
    17821 tagggctgag cagtatgacg gtcagtattt tgtttattct gccgattccg gcagcgtttt
    17881 ttggcgcatg gtttgccgga cgtcccaata aacgcttctc cacgttaatg tggcagtcgg
    17941 ttatctgctg cctgctggct ggcttgctga tgtggatccc cgactggttt ggcgtgatga
    18001 atgtctggac gctgctcgtt cccgccgcgc tgttcttttt cggtgccggg atgctgtttc
    18061 cgctggcgac cagcggcgcg atggagccgt tccccttcct ggcgggcacg gctggcgcgc
    18121 tggtcggcgg tctgcaaaac attggttccg gcgtgctggc gtcgctctct gcgatgttgc
    18181 cgcaaaccgg tcagggtagc ctggggttat tgatgacctt aatgggattg ttgatcgtgc
    18241 tgtgctggct gccgctggcg acgcggatgt cgcatcaggg gcagcccgtt taagcgcacg
    18301 tcaccgcagc atcgtcatca gctccatggg agaacgatgc tgctttatca gatcacgcat
    18361 cacccgcata tgcggtgcgg agtaagaata aaacgcctga tagcccgcac aaagcacgct
    18421 gtctggcgtc tcctgccgag cgtgcgggca caggcgatgc cagtcgcacg cctggcagtc
    18481 acggcgcggt tgcgggtttt cgctgccaca ccatttttgt aaggtggcgt caaaaagctc
    18541 aatcgagata cgactgatat ctgcccgcac ccaacactcg aacagggcaa ttaaaaattt
    18601 cccccacagc tctgctgtca ccgaagcggc cgtcagatcg ccccggtcat caaattcaag
    18661 atagggaatg agctgtacgg agcggatgcc ccgctgtttg aaattgtgat aaaaatcggc
    18721 aggttgcaga gcatccttgt actctacaac cagtttatgc ctgtcctctt cagcatcaat
    18781 gacctgactt cctgtcatgc gactctctca ataaatacga taagttgatg tatatgcgtt
    18841 acagcaaaca gtattgcgag agataacacc gcgagggaga tcaaaaattt gtcacggact
    18901 acgtgaaatt gggaaaaatc gctattcgtg acatccatta aattgcgatt acgggtgtag
    18961 tgccagagga tcagcgccac gatggcgaga atgccaatcg aaatccagaa taacatgccc
    19021 gcctggtgcc agttgtgttt gatagccaac gccatcaacg cgccgtagcc cagcatggta
    19081 cgaaaccacg ccagtgatgt gcgctccggc tgcagtcctg ggtcggcaat gcgtctggct
    19141 ttgcggctat ccggcataca acaccagtcc cataacgatc accgccacga ccatcaatat
    19201 taagctaatg attaataagc tgttggtgta gggcaggtcc tctttcagac gcatcgcttt
    19261 ttcattgcgt aaccagcgca ggtagccata cattgctaac ccgccggaaa acagacacaa
    19321 caacaacgcc agcagttcac gaatgacggg cgtggcgaaa tctggcgcaa gttgatcaag
    19381 cccaaccccc gccgccagaa atcctagtgc ggtacggatc cacgccagaa aagtacgctc
    19441 attagccagc gagaagcggt aatcgggcgc ttctccaagg cgggaaatat tcattgttgc
    19501 tcctttgcaa tttcactaac gctctatctt tagcggtttt ttttaagaat ttcgtccgcc
    19561 cgtttcctga tatgcttttg ccatccgcta caaggaggtc agatgggtat catagcgcaa
    19621 attgacatcc tccacgccct gaaggacgtg gatccctgct acgttcaggc tgtcgcctga
    19681 atcatttcgg tgggttcctg cttcaacgag cggcctgact gcaccatctc tccacaggca
    19741 agaacggcgt gccccgccgc taaaatgtta cgagcgccat ttacatcggc gttcgctgta
    19801 tatccacata cctggcatct gaatttactt tgtgacaggc gattttcttt cgctgtatga
    19861 ccacagcaca cgcaacgctg gcttgtgtac gctggcggaa cagcaagcac ctgaccacca
    19921 cgccagagct gcttgtacgc aagctggcgg cgtatttcat accagccctg atccagtatc
    19981 gaacggttta aacctgattt tgcccggaca ttgcgccccg gcaggctgac cgtacccgct
    20041 gctgactttg acatgttgct gaccttcaaa tcctcaatga ctatcattgc gtggtttttg
    20101 ctgacggtcg ttgtgacttt gtgaaggtag tccctgcgga tatttgcgat acaggaatgc
    20161 agtcgctgta ttttgcgttt ctgcttctgc cagttgttgc tgaatttgac cttgcggctt
    20221 aactgtcgct gaagtctcgc cagcgtcttc tggtttttct gaaaactgtt tacaggctca
    20281 aagactgtgc catctgacag cgtggcgagt ttagccacgc cagcatccag cccgatcatt
    20341 gatgctgaag ggtgaaccgg agttgatact tcactttctg tctgaatact gatgtaccac
    20401 ttaccgcagg actggctgac agtgacattt ttcacaacac ccgtgacctg acggctgttc
    20461 cggtagcgca tccagcccag tttcggcaga aaaatacggc tgttttcctg atcgagctta
    20521 acgccctgcg ggtagcggaa tgcatcattc tgtccccgct ttttgaatcg gggaaaagca
    20581 gcccgcttct ggaagaagtt tttgtaagcc cgctcaaggc ctttcagtga ctgttgcaat
    20641 ggctgtgagg gcgaatcttt aagccattgc gtttcagtgg cgtttttcca ctcaaccagc
    20701 caggaagcca ttttcccgta agggatgtat ttattaccgg cctcatgatt ctcattctga
    20761 agcgccagag cacgattgaa aacgaaacga catgcgcctg cgaagcgcct catttcacgc
    20821 tcctgttgac caccgggtct taactggaat ttaaatgcct gtaatcgttt cacaggccaa
    20881 atgatacttt ggtctatgaa taaagaaacc gatatccgac ggggaagcat tgtgttttcc
    20941 tgatgcatgt tcacctggtc tttgtcacca gataccgacg tcagattttt gactatgacg
    21001 cgacagaaaa actacgcact tacttttcaa atgtatgtgc tgattttgaa gctgaactgg
    21061 tttaaatgga tggcgaacca gatcacgtcc atttgttaat taactatcct cccaaactgg
    21121 cgatatccag tctggtaaac agcctcaaag gcgtatcggg taggttactg cgacgagatc
    21181 gaccagatat tgcagtcagg tattactaca aaggcgtttt gtggagtcct ggctattttg
    21241 ccaatagctg cggaggtgcg ccaatatccg tcatccgcca atacattgaa caacagcaaa
    21301 cacctggtta ggtggaaaac cgcgccttat atccccgccc tgaaggacgg ggatataagg
    21361 cgcaccggat aaatttggtt atgcgccgtc agccgtgcta aagaatacgg accagcaccc
    21421 aacagatgcc agtccacaca attaacgtaa ctctggccag tacgctttgt tggcctccag
    21481 caattcatcc agaatagctt tggcgaccga ggcgctcggt accgttttcg acaacgtaat
    21541 cgcctgccac aggtgctgat atgaacgctg ttcccaggcg tccacgacca gtttttccac
    21601 cgccacttgc tgactcatta accctttctg aaactgcggg atatcgccga ccaccagtgg
    21661 ttctggtccg ttgtgcccaa ccagacacgg gatctcgacc atcgcttcat catcaaagtt
    21721 atgaattgcc ccgttgttag gcacaatcag caacatccgc tcctgagtgt tgaaggcaat
    21781 tgccgccgcc agatcgacga tgtatgacgc atgttcgtca atttccagct tgcccgccgc
    21841 tgaatttcct gccgccgtaa tggcgcggca agcatcgaaa acctgtttct cgcgatgttc
    21901 catcacctca ttcgcgcggg tatgttcagg gttggaatgc tgaacgacgt aatccgggaa
    21961 gagataatat ttcagatagg tgtttggtaa tgtatccggg gccagtgcct ggacatcccg
    22021 cgcttttgca taggtgtcgt tccagctcgc ttcagtatgc gtatcttgct gcggtggaac
    22081 ataaccatgc ttagagacat attgccgcag ctggggcatc aggtcgttgc cctacaaatc
    22141 ttcaattgat gtccaccagc caaagtggtt caggccgtag tagcgcacgc gcatctgttt
    22201 gcgatcttgc agcccaacaa tttgcgccat ccggctttca ataccgattg gcatgtcaca
    22261 gatgttgagg attttcgcat tcgggcgcag acgacgcgtg gcttctgcga caatggctgc
    22321 cgggttggag tagttgagca tccaggcatt tggtgaatat ttttccatat aatccaccag
    22381 ttccaggacg ccgccaatgg aacgcatgcc gtacgctatt ccgccgggtc cgcaagtttc
    22441 ctgaccaaca acgccgtggc gcagcgggat tttttcatcc agttcgcgca tcgggtattt
    22501 gccgacgcgg atgtgcgcca tgacaaaatc aacgtcgctg aatgccactt caggatcggt
    22561 ggtgtaacta aacgcaatgt ccggcgcttt ttctttaagg atgactttac aggcttcggc
    22621 aatcacttcc tgccgcgcac catcgttatc ataaaatttc agtgcacgaa gcgggaaacg
    22681 gtcctgattc gccaggagca tcaacacgat gcctggcgta aaggtgcttc caccgcctgc
    22741 gacaaccact gagaatttgg tcattatact gcctccgtaa tggcaacatt ttctgctgat
    22801 tgatgagaat taattaagct atcgagctgt tcacgcagct gagatacatg cagaccaatg
    22861 atcacctgaa tggcatcgcc actacggaag acgccgtgcg ctcccagctt tttaaagact
    22921 tcgtcatcca gcgtttgtga catgtcatgc agtgcaatac gtagacgcgt cgcgcaattg
    22981 ttgatgctgg agatattgcc gaccccgccc agggcttgca ggataccggc agcctgatcc
    23041 aactcttttt ttggctctgc agcggtggtt tggcctcgcg aggctttata ttcggctttt
    23101 gagtagagtt tcacttccgc atcttcacgt cccggcgtac acatgttgaa ctgcagtatc
    23161 agggtgcgga aaaccacgaa gtacagcagg gtaaagcaca acccaatggc gatttgggtc
    23221 agcatcatat ccgcgtggtt gctgaacatc gggatccagt tttgcggtaa aacctggtca
    23281 atcagacctc cgcccatgtt gcccaccaca ccaaagagat acattacggt cgacattgag
    23341 gccgcaagca cagcgtgtac cgcaaacagc aacggtgaaa tgaacaggaa ggtaaattcc
    23401 agcggttcgg taattcccac cagcatggcg gttaaggttg cggggatcag caagcccgcc
    23461 acttttaccc gattttccgg tgcggcagtg aagtacatcg ctaaagaaat gcccacggca
    23521 ccaaagattt ttgagttacc gtgcagggca aaaccgcctt ccgggaacaa cgatttcagc
    23581 ggctcggcgc tcagactgaa ctcttgcaga tgctgcgccc agtacatctg aatgccgcct
    23641 tcaacagctg ccggaccaaa gataaacggt ccgtagatga agtggtgtaa accggttggg
    23701 atcagaatac gttcgaggaa ggtgtaaacc cacaccccaa gcgcacccgc cgaacgcagg
    23761 aacgcttgca gagattcaat ccccatttgt acttttggcc agccgagcaa tgtcagccag
    23821 gcacagggga tcatcaccag gaaggcgata atcaccacat aagacgtccc ctggaaaatg
    23881 ccgagaaaca ccggcagttt tttatcgaac agacggttat gcagcgccgt cacaatgccg
    23941 gaaatgataa ttgcgccgat aatgctggta tcgagggttt taatcccggc catcattgtc
    24001 agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc cgaagtaact tccccaggtc
    24061 attcccatcg cattaatgaa atagttccag gtcaggaaac tcaccatcac cgccagacaa
    24121 gcacgcccct gcgcttgctt agcaaggcca atgggtaaac cgacagcaaa aatcagcggc
    24181 atattacgga ataccgtcca accgccctct tcaataatgt gtacgatttg cgcgaataaa
    24241 ctgttcggat cggtcagtga ttccccgaca aacatcgggt tttgcaacaa gatggcaaga
    24301 cccaccacaa tcccggcgaa gggaaacagc agcactggcg tgaacatcgc gccgccaaag
    24361 cgttgaattt gactgagcat ttccacatcc tcttttctca attctgtaag taggaagcct
    24421 gattggttat gcggcaggcc tggagcgagg ataggaagat gcgatcggca gaacatgacg
    24481 acccaatggg attcgtgatc gctttcatgc ttttcgttgg gcggtagaga tctacgtttt
    24541 cgcgaaaaag tagacctctg tgagcacagg tacacagcat cgaacacatc tttaaaaaaa
    24601 agatgttttt tcaatcgatt aagcagaact tgtgtgcgca ttacccgggc ttgcaggcaa
    24661 aaaagagatc tagagatgat ctacaaaagc attgcggagc ggttaagaat tcgacttaac
    24721 tccgcagatt tcacgctaaa cagccttctt cccggtgaaa aaaagctggc ggaagagttt
    24781 gcggtatcgc ggatgaccat ccgtaaagcc attgacctgc tggtagcgtg ggggctggtg
    24841 gtccgccgcc acggtagtgg cacttacctg gtgcgcaaag atgtactgca tcaaaccgcc
    24901 agtctgaccg ggctggtgga ggtgttaaaa cggcagggaa aaacggtcac cagccaggtg
    24961 ctgatttttg aaatcatgcc tgcgcctccg gccattgcca gccagttacg gattcaaatc
    25021 aacgagcaga tctacttctc ccgtcgcgtt cgttttgtgg aagggaaacc gctgatgctg
    25081 gaagacagct atatgccggt aaaactgttc cgtaatcttt cgctgcaaca tctggaaggg
    25141 tcgaagtttg aatatattga acaagagtgc gggattttga ttggcggtaa ttatgaaagc
    25201 ctgacgccag tgctcgccga tagactgctg gcgcggcaaa tgaaggtagc ggaacacacg
    25261 ccactgctgc ggatcacctc gttgtcatat agcgagagcg gggagttttt gaattattca
    25321 gtgatgttca gaaatgccag cgaataccag gtggagtacc atttacggcg actccacccg
    25381 gaaaagagtt aaccgatact ccagaagagc accgccagta attggggggt gataatgcgc
    25441 aggaacatta ccaacggata gacagtggcg taagagagcg ccgccgcacc gctggttgga
    25501 tgaagattat tagcaaacgc cagcgccgga ggatcggtca tggaacctgc cagcatcccg
    25561 cacatggtca ggtaattcat tttggctaac atccgcgcca gaatgccaac agtaatcagc
    25621 ggaacggcgg tgatcagggc accataacca atccagctta gcccttcgcc attgaccagg
    25681 gtattcacaa aatccccacc agatttcaga ccaacgaccg agaggaacag cacgatcccc
    25741 agctcccgca gcgcgaggtt ggcgcttggc ggcataaacc agtacagctt gccgatactg
    25801 ccgatacgcc cgaggatcaa cgccataatc agcggtccgc ccgccagccc cagtttcaac
    25861 gcggccggga atcctggcac aaagacggga atagaaccta acaatacgcc tagcccgatg
    25921 ccaataaaca ctggcagcat ctgaacctgt tgcagttttt gctgcgcatt ccccagcaca
    25981 ttggcaacgg catcaattgc ggacggacgc cccaccagat tgaggatatc gccgaactgc
    26041 aggctgatat cgccactggc gaccagttcg accccggcac ggttcaggcg cgagataaca
    26101 acgtcatagc gttctttaaa gtgcaggtcg cgaatacgtt ttccgagcac gttttcattg
    26161 gtgaccacca cacgctcgac gcgcaaatca gtgcctttcg tggacagcga agtatcgacc
    26221 tcctgaccaa tcaccagttg cgcattatgt aaatccgctg gctgacccac cagatgcagc
    26281 aaatcgccca gttggataat ggtatctggc gaaggaactt ttagggtttc ttcgcgtttc
    26341 agacgcgagc agataatttt gtcgccgttg agaatcggta catctttaat ggctaaatca
    26401 tgcaggttag ggttctcaac gcgaatattg atagtcttga tcagcgcgcc gccattggtg
    26461 cgtgaagact cgtgctgctg agcttctgtc tcgacattga cgcggaaaat aacccgcaac
    26521 atccacatgg tgaacaaaat cccgcaaatg ccgaatggat acgccatcgc gtaactcatc
    26581 cccatctgat cgaccatttc cattggtgta cccaggtcgc gcaaaatctg ctgccctgcc
    26641 cccagcgctg gcgtattggt aaccgcaccg gagaaaatcc ccagcactac cggcagtgga
    26701 atatcaaaca gtttatgcag gatggcggta accagaccac cgatgatgac gatcagaaca
    26761 gcaaacaggt tgaggcgtaa tccggagacg cgcaatgagg caaagaagcc cggccctacc
    26821 tgaatcccga tagtataaac gaacaggatc aggccaaatt cctgaataac atgcagcata
    26881 tcgctactta atgtcatccc cgcctgagaa acaaaatggc cgacgatgat cccaccaaac
    26941 agcacgccgc caatacctaa tcctatgccg cgaaatttga cgttgccgat aaacaaaccg
    27001 acgactgcca ccaaagccag aatactgacc gttaatgcta tatcactcat cgtccatttc
    27061 tcttgagata acattttaat tgctacggat tatggctgaa tcggtgaagc agatttgcct
    27121 tatagcgcac attatcacgg gaaagaacgt gccgaaatat cttaaacagt cgaaatttaa
    27181 cgcattgttg agtaagtaag acaaaaaaag ccccaccaga atggcggggc aaagagaata
    27241 gctagttagc tatttaacgc gggacgttcg ctgatagcga tacgctgcgc tgcgatgggt
    27301 tcaggctcat tacgaattaa atcaatatgc agtaaaccgt ttacgaaggt tgcgccagag
    27361 acttccatat tttcagccag cgtaaagctc aggctaaatg gctgattcat aagcccttga
    27421 tgcagccatt ttttctcttc ttttggctgc tccggcgtgc cttttacgct caggcgcgta
    27481 ccttccagtt gaatctctaa atcttcctga cggaaacctg ccagcgcaag ggtaatgcgg
    27541 tagtggttat cgtcgctttt ctcaatgttg tacggcggga agctctggct ttcaccggcg
    27601 ttttgcagtg cgttggccag tttgtcaaaa ccgatccatt gacgcatcag tggggataaa
    27661 tcgaagttac gcatagtcat ttctccttct aagaagcgag taagtacctg caaatccgaa
    27721 gattcgcata tgctccctga cggcgagcat ggagatgtca ggccgcgcca ggcggcctta
    27781 gggaattagt tgatttcgat acggcgcggt tttttcgctt ccggaatcac gcgttcgaga
    27841 tcgatataca gcaaaccatt taccaggtta gcaccacgaa catgaatgtt ctcagctaac
    27901 tggaatttgc gttcaaagtt gcgttcagcg atgccctggt acagataggt gcgctctttt
    27961 tgttcgtcgg cgtgagcacc tttcaccacc agcagattat cctgggcggt aatttccagt
    28021 tcgctctcag caaaaccagc cacagcgata gcaatgcggt aatggttttc gtctaccagt
    28081 tcaacgttat acggagggta gccgccatta ctctggctct ggttgttttc taagtggtta
    28141 aacaaacggt caaatccaat agcagaacgg taaagcgggg ataaatcaaa gttacgcata
    28201 atcaatagct cctgaaatca gcgagaatgt aagaccttcc acaatggaca ggtcaggtag
    28261 ccagaacacc catcaggcgc gtcctcatcg gctacgatgt aaaaatgggt ctggaaatga
    28321 acttttcaag ccttatcagc ggactttttt ttgcagttta tggtctattg catagactga
    28381 gggggcagca cgaaacgtta aagtgcgata gccgccacag tgcgacgtaa tgatgatgtt
    28441 attttcagca agggatcgct ataactcatc aggccaacac ccacgatcaa cagatgaatt
    28501 tattatgata agaaatgtgt tgttagcgtt catgatatgc agcggaatga cattactcgg
    28561 aggatgctcc agcgtgatgt cccataccgg agggaaagaa ggaacgtatc ccggcacccg
    28621 cgccagcgca accatgattg gcgacgatga aaccaactgg ggcactaagt cgctggcgat
    28681 tctcgatatg ccgtttaccg ctgtgatgga cacgctttta ctgccgtggg atgtgttccg
    28741 caaagacagc tcggtgcgct cgcgcgttga aaaaagcgaa gccaacgcgc aagccaccaa
    28801 tgcggttatt ccaccggcca gaatgcctga taactgattt agcgatcagt ttcggtgatc
    28861 cacagctggc cgccttccat ccacgccagc cactgaccat ccggcgagaa gaccacggcg
    28921 tccgcagaag gtgaattggc gtggtgttcg gttaaatact caacctcgcc gctttgcgca
    28981 tgggcacaag caattcgatt atccagcaca aagcccaacc attctcctga cggatgccag
    29041 ttaaatgcag actgaatatc cgttttgtta tgggttaact ggcgcggctc gccgccctgt
    29101 ggcgagataa gccacagttg cacaatgccg ttatcatcac gcattaaaaa cgcgatttgc
    29161 gtaccctgcg gattacagcg cacccagtgg cgcgggacgt tgactaaccc cggataagcc
    29221 cgatggtggg taaaggttaa acgtcgctgc acgacgccgc gcggtggcgc gggcagggtg
    29281 gtttccgttc cacttaacgg cgcatcacct gccgctttcc agccagcttc atcttgcggt
    29341 aactcaacga taaacagctc cggcactttc tcgccctttg gcgaaagtgt gtcgccaata
    29401 aacgccagcg cgtgatttcc tacccatcct tcttcataag cacgattgat ttcatcgctg
    29461 ccaggctgcg gcgtgggcgt ggttttactc accagcacgc accagtggct accgctgtat
    29521 tcacgcggat gctgcttttg tacgttgacc gggccaaacg gcgcagcaac accgacgttt
    29581 cgcaaatcca gcgccggatc gagttcatgc attacatggt cgttataggt aaagctcacc
    29641 ctttcaccgt tcgggctaaa gacatgcaca tggctgccac cgcgcagcgc tcctggcgta
    29701 tacggcgcgg taatatccat tgcatcgaga ttgctcacct tgccgccttc agcaatgact
    29761 ccgcgtcgat gatggaagtc gtaatgccat gtttcatcag gattttccgg gccatggatg
    29821 aaaacatatt tctctgactt cggatgaacg gtcaccacgc cgacgtgtgc gccctgtgac
    29881 gcgcgataga taacctcgac ctcgcctgta tggatattca cacgctcaat ggtctcgccg
    29941 gtaaacgacg ccccagaagg acgcacgtca aataccagcc actggctgtc gggcgtccag
    30001 gtattggtat tggtgagcag gtgattacgg ggagcaaagg ttatctgttt catggcgcgg
    30061 tcctgataca aaacggttcc cgccatcata cttcacaagg acttcacttt cagacaatag
    30121 tcttttgcca tcgtgatgaa tgtgagtgaa aagatggaac atttcgacgt ggcgattatt
    30181 ggcctcggcc cggcaggatc ggcgttggcg cgaaaattag caggcaaaat gcaggtgatc
    30241 gcgctggata aaaagcacca gtgtggtact gaaggtttca gcaaaccttg tggcggtctg
    30301 ctggcaccgg acgcgcagcg atcttttatt cgcgatgggc tgacgcttcc tgtcgatgtg
    30361 atcgctaatc cgcagatttt cagcgtcaaa accgtcgacg tcgccgcatc gctcacgcgt
    30421 aactaccagc gaagctatat caatattaat cgccatgctt tcgacttgtg gatgaaatcg
    30481 ctgatccccg ccagcgttga ggtttatcac gacagcctgt gtcgaaaaat ctggcgtgag
    30541 gatgataaat ggcatgtcat ttttcgtgca gacggctggg agcagcatat taccgcccgc
    30601 tatctggtcg gtgccgatgg tgccaactcg atggtgcggc gacatctcta cccggatcat
    30661 caaatccgta aatatgtcgc aatccagcag tggttcgcag agaaacatcc ggtgccgttc
    30721 tactcctgca tctttgataa tgcgataact gactgttatt catggagtat cagcaaagac
    30781 ggttatttta tctttggcgg tgcctatcca atgaaagacg gtcagacgcg tttcacgacg
    30841 ctgaaagaga aaatgagcgc ctttcagttc cagtttggta agacggtgaa aagcgaaaaa
    30901 tgcacggtgc tgtttccctc gcgctggcag gattttgtct gcggtaagga caacgccttt
    30961 ctgattggtg aagcggcggg atttatcagc gccagctcgc tggaggggat tagctatgcg
    31021 ctggatagcg cagacattct gcgttcggtg ttactgaagc agccagagaa gctcaatacc
    31081 gcttactggc gcgccacccg caaactgcgt ttaaaactct tcggcaagat agtaaaaagc
    31141 cgatgcctga ccgcaccggc tttaagaaag tggattatgc gcagtggtgt ggcacatatt
    31201 ccacagttga aggattagcc aacgcgcttc acatcgccca ccagcaggat ataagagagc
    31261 gcgccaatca acgcgacggc ggagatataa accagtgcag gtgcgaaacc gtaaccctgc
    31321 gccaggtacc ccaccaccag cggaacggtg atgccgccca gaccaccggc gaagttaaac
    31381 acgccgccgg ttaaaccaat caggcgcatc ggtgccagag aagaaaccag cgaccaggta
    31441 atcgaagcaa aaccgttacc gaagaatgcc agcgccatca ggcacataat catcatcgga
    31501 tcgttagtgt agttagcgcc cataatgcag gtggagatca gcaagccgca gataatcggc
    31561 gttttacgcg caaagcccag tgaaaagccc ttacgtacca gcagatccgc tacccagcca
    31621 gagagcagga cgccgacaaa cgccgcgagg aatggcaccg tggtcataaa gcctgctttc
    31681 agcgccgtga ttcctttttc ctgggttaaa tagttcggga accaggttaa gaaaaaccac
    31741 agtgtagaag ccaccgcaaa ttgcccaaga taaacgccga tcagtttacg atggaacacc
    31801 agtttccagt ctttggctgt taacggctga cgcgcctctt tcttcaccgg cgcatcacca
    31861 tccaccagac cgccgccatc acgaatgtaa tccagttcag ctttgctgat acctttggtc
    31921 aggcgcggcg gctgataaac cttaaaccaa atcagcgacc agataatgcc gataccacca
    31981 gtgacaatga acacccagtg ccagctcaac atctcctgaa tccagatcag cagcggcgtc
    32041 agaaacgcca gaccgacaaa ctgaccagac gtataaaaac caacggcaga agcgcgttca
    32101 tgttccggga accagctggt caccatccgg ttattggtcg ggaaagcagg cgcttcgaaa
    32161 ataccggtta tcgcgcgcag accaattaat gacattaagc ccgtggcaaa gccctggaat
    32221 aaagtcgcca ctgaccagcc aaatatcgca ataaaataag tcacgcgaga acctacgcga
    32281 tctaaaaacc aaccgccggg gatctgacat agcgtataaa gccaggcgaa ggccgaaaat
    32341 acatagccca tttccgcttt ggtaatgccg aactcttcct gaatatgggc ggaagccacg
    32401 gccaggttgg cgcggtcgac ataacaaatg actaccgtaa taaagatcat caccagcgtc
    32461 agataacgcc gacgccccgg ctttgctgca ttaacgggaa tatccatagc gagctttctc
    32521 cagattttgg gcatagcgaa gccgctcacc atgccctgta atttacagag ggttatattt
    32581 ttgtattgct gttttagtgc ccgatgaggg gcttacgtgg caggaattac cactctgcta
    32641 cgctgttatc ttcatgacgc cagagcggat tacgccagtc cggggcattt ttactgaact
    32701 caatcacttt agcttcgtcg atttccacgc ctaagcccgg tttcgttaac ggtttaaaga
    32761 agccgccgac catgctgaag tcttctttgt ttttcacaaa gtcgagtaac tccgcgcctt
    32821 tgttgtaatg aattcccata ctttgttcct gaagtacggc gttataggaa acaaagtcga
    32881 tatgcaggca agccgccagt gcaatcggtc cgagcggaca gtgcggcgca agggtcacgt
    32941 cataggcttc tgccattccg gcgattttgt agcattcggt aataccgccc gcgtgggaga
    33001 gatccggttg cagaatcgaa ataccacctg cctccagcac gcgtttaaaa tcgaagcgtg
    33061 agaacatgcg ttcacccgcc gccagtggaa tatgcgtttg tgccgccagt ttcgggtagt
    33121 attcggcttg ttccgccagc accggctcct caataaacag cgggcgatac ggctccagtt
    33181 ctttaatcag cactttcgcc atcggcgcgc tgacgcgacc gtggaaatca agaccaaact
    33241 caatctgatt gccaaaagct tcacgaattt gtgccacggt gttaaccgcc gcatctaccg
    33301 cgcgggagtt atcaattggc cccagttctt cacaaccgtt cagtttgaag gtatcgaagc
    33361 cgatttcgcg tagcgtttta atgccgtcga taacatccgc cggacgatcg ccgccaaccc
    33421 aactgtaggc tttaattttg tcgcgaacca ggccgcccat cagttgccag accggcgcat
    33481 tcagcacttt acctttgata tcccataacg cctggtcaat cccggcgatg gcgctcatca
    33541 ggatcggacc gccgcgatag aatccggcgc gatacatcac ttgccataag tcattgatgc
    33601 gcgatggatc ctgaccaatc aaatagtcac ccagctcgtg aactgccgct tccaccgtac
    33661 gggcgcggcc ttcgatcacg ggctcgcccc aaccgaccac gccttcatcg gtttcaattt
    33721 tcaggaacat ccagcgggga ggtaaacgat acgtggtaat tttggtgatt ttcattgcac
    33781 tgcctctcga tacgccttaa caaatgctgc tgcctgctgc gcggtgcgct ctacggattg
    33841 cccggcgcga tagagatcgc tgcctaagcc cgcccctgca caacctgcgt ctatccactg
    33901 cgccaggttt tctggcgtca cgccgccaac ggcaaagact gcgatgtccg atggcaatac
    33961 cgcttttaac gctttgatgt attgcggacc aaaagccgat gacggaaata ttttcagcgc
    34021 ctgcgcgccc gcttcgagcg cggtaaaggc ttcggtcgcc gtcgcgcagc cggggcagac
    34081 ggtcatgccg tagcccaccg cacggcggat cacttcacta tggatattgg gcgtaacgat
    34141 gagctgacag cccatcctgg cgagcgcatc gacctgttca ggtttcagta ccgtacctgc
    34201 gccaatcaac gccttgtcgc cgtacgcatc aacgatggcg ggaatgcttt gctcccattg
    34261 tggggaattc agcgggattt caaccgcgtc gaacccggcg tcaatcaccg cgccaacatg
    34321 cgccagcgcc tcgtcgggcg taataccgcg caaaatggcg atcagcggga gtttagtttg
    34381 ccactgcatg agcgatgctc cttataccag cctgaaatgc cgtgtcgccc gccaccgccg
    34441 tcacgtcgca acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc gatgttccgg
    34501 cgacaagggt gatggcgtgt tgatgggcca catagtcgcg catactggcg acctctgcgc
    34561 caatcaacaa accagagaga aattcgctga cctgttcgcg gggaagtgtt cccagcacat
    34621 gcgaggcgcg aacttcaaaa agctgcggca atatggcggg cgtattaaga ccacgctcaa
    34681 ggccagctgt gaaggcatcg gcagagtttt cctgcggcgg caaacctgcg ccaatcaatg
    34741 agtgatttaa cagtaaatga tgtaattcac cggtcatcac ggtgcgaaaa tcgttgattt
    34801 gctggctatc ggcctgcacc catttgcaat gggttccggg catgacataa agagaggaag
    34861 gagccagagc tcgcgcgccg atcaattgtg tttcttcgcc gcgcatcaca ttgtggttat
    34921 cgtcatgaga gacacataat ccgggaataa tccagatatt gtcgccaact gacgttaatt
    34981 gttcgccaat agacgaaaaa caggcaggaa cagataaata cggtgcaact ttccagccga
    35041 cgttgctgcc aaccattcct gccattacca ctggcgtttt ctcttcacgc cagtcggtcg
    35101 tgacttctgc taacaccgca gccggagatt ttccgttcag gcgcgtgacg cctgcttctg
    35161 attgcctgct ctccaggcag tggtcgctct gataaagcca ggcgcgcaga ttggtcgatc
    35221 cccagtcaat tgcgatgtag cgagctgtca tgtgatttcc tttaaccttc gtgtcgagct
    35281 ggcgatcatg gtaagcgccg cctgctctgc cgcatcgccg tcctgatgtc gtatcgcatc
    35341 gaacagcgcc ttatgttcct ggagcgtttg cggcatgttg gcctcatcgc ccatccaggt
    35401 tcgttcaaaa accgcccgct gcagcgaact gatcgcaatg ctaagttgct gtaacaccgg
    35461 gttatgcacc gactgcagca ccgcctcgtg gtagcgaata tccgcttcgt taaacgcttc
    35521 gcggtcctga ttgttggcaa tcatctcgtt cagcgccgat tcaatctgcg ccagatcgct
    35581 ggaagtcgcg cgctctgctg cccaacgggc aatcgccggt tccaccagat ttcgcacttc
    35641 gctcatggca ctgataagcc gtgggtcgta gtcattttcc agcacccatt gcagtacgtc
    35701 agtgtcgagg taattccact ggttacgcgg tgccacaaac gccccgcgat aacgtttcat
    35761 ttcaatcagc cgcttcgcca tcagcgaacg gaacacctca cggatgatgt tgcgcgaggt
    35821 tgcaaactcc tcacagagtt ccgcctcagc cggaagcggc gagcctggca cgtatttgcc
    35881 gtgaacgatc tgtttaccca gcgtaatgac aatgcgatcg gttttattga gagtcatgga
    35941 gagtccttgt gctctgtatg ttcttctcta ctttaccccg atcgatgcat aacgcggcaa
    36001 ctttgtagta ccagcgtgat gacgttcgcg tttgccgtgc gtgtaatgta gtacaaactt
    36061 atattgttgt actacaattt agatcacaaa aagaacaatg cataaaaaaa tgacatgcgt
    36121 cgggcagaaa tctgaaaagg gatatcaggc gctaaacagg agggaaagaa gagtatgctt
    36181 tcaacggctt agctactcgt ttaaaggatt aatcatgaag ttgaatttta agggattttt
    36241 taaggctgcc ggtttattcc cactggcgct gatgctttca ggctgtatct cgtatgctct
    36301 ggtttcccat accgcaaagg gtagttcagg aaagtatcaa tcgcagtcag acaccatcac
    36361 tgggctatcg caggcaaaag atagtaatgg aacaaaaggc tatgtttttg taggggaatc
    36421 gctggattac cttatcactg atggtgccga tgacatcgtt aagatgctca atgatccagc
    36481 acttaaccgg cacaatattc aggttgccga tgacgcaaga tttgttttaa atgcggggaa
    36541 aaagaaattt accggcacaa tatcgcttta ctactactgg aataacgaag aagaaaaggc
    36601 actggcaacg cattatggtt ttgcctgtgg tgttcaacac tgtaccaggt cactggaaaa
    36661 cctaaaaggc acaatccatg agaaaaataa aaacatggat tactc
//
LOCUS       DAJOSY010000034        35867 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJOSY010000034 DAJOSY010000000
VERSION     DAJOSY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.034"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(81..812)
                     /gene="rlmB"
                     /locus_tag="OHN27_001820"
     CDS             complement(81..812)
                     /gene="rlmB"
                     /locus_tag="OHN27_001820"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCQ2286271.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(992..3433)
                     /gene="rnr"
                     /locus_tag="OHN27_001821"
     CDS             complement(992..3433)
                     /gene="rnr"
                     /locus_tag="OHN27_001821"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCQ2286272.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPSTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDVGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3472..3897)
                     /gene="nsrR"
                     /locus_tag="OHN27_001822"
     CDS             complement(3472..3897)
                     /gene="nsrR"
                     /locus_tag="OHN27_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCQ2286273.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4102..5400)
                     /gene="purA"
                     /locus_tag="OHN27_001823"
     CDS             complement(4102..5400)
                     /gene="purA"
                     /locus_tag="OHN27_001823"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCQ2286274.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5504..5701)
                     /locus_tag="OHN27_001824"
     CDS             complement(5504..5701)
                     /locus_tag="OHN27_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCQ2286275.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5783..6787)
                     /gene="hflC"
                     /locus_tag="OHN27_001825"
     CDS             complement(5783..6787)
                     /gene="hflC"
                     /locus_tag="OHN27_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCQ2286276.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6790..8049)
                     /gene="hflK"
                     /locus_tag="OHN27_001826"
     CDS             complement(6790..8049)
                     /gene="hflK"
                     /locus_tag="OHN27_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCQ2286277.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8135..9415)
                     /gene="hflX"
                     /locus_tag="OHN27_001827"
     CDS             complement(8135..9415)
                     /gene="hflX"
                     /locus_tag="OHN27_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCQ2286278.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9491..9799)
                     /gene="hfq"
                     /locus_tag="OHN27_001828"
     CDS             complement(9491..9799)
                     /gene="hfq"
                     /locus_tag="OHN27_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCQ2286279.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9885..10835)
                     /gene="miaA"
                     /locus_tag="OHN27_001829"
     CDS             complement(9885..10835)
                     /gene="miaA"
                     /locus_tag="OHN27_001829"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCQ2286280.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10828..12675)
                     /gene="mutL"
                     /locus_tag="OHN27_001830"
     CDS             complement(10828..12675)
                     /gene="mutL"
                     /locus_tag="OHN27_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCQ2286281.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12685..14022)
                     /gene="amiB"
                     /locus_tag="OHN27_001831"
     CDS             complement(12685..14022)
                     /gene="amiB"
                     /locus_tag="OHN27_001831"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCQ2286282.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14041..14502)
                     /gene="tsaE"
                     /locus_tag="OHN27_001832"
     CDS             complement(14041..14502)
                     /gene="tsaE"
                     /locus_tag="OHN27_001832"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCQ2286283.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14474..16021)
                     /gene="nnr"
                     /locus_tag="OHN27_001833"
     CDS             complement(14474..16021)
                     /gene="nnr"
                     /locus_tag="OHN27_001833"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCQ2286284.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16020..17159
                     /gene="queG"
                     /locus_tag="OHN27_001834"
     CDS             16020..17159
                     /gene="queG"
                     /locus_tag="OHN27_001834"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCQ2286285.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17142..17195)
                     /locus_tag="OHN27_001835"
     CDS             complement(17142..17195)
                     /locus_tag="OHN27_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286286.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17429..17504)
                     /locus_tag="OHN27_001836"
     tRNA            complement(17429..17504)
                     /locus_tag="OHN27_001836"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17469..17471),aa:Gly,seq:gcc)
     gene            complement(17540..17615)
                     /locus_tag="OHN27_001837"
     tRNA            complement(17540..17615)
                     /locus_tag="OHN27_001837"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17580..17582),aa:Gly,seq:gcc)
     gene            complement(17773..17848)
                     /locus_tag="OHN27_001838"
     tRNA            complement(17773..17848)
                     /locus_tag="OHN27_001838"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17813..17815),aa:Gly,seq:gcc)
     gene            complement(18059..18604)
                     /gene="orn"
                     /locus_tag="OHN27_001839"
     CDS             complement(18059..18604)
                     /gene="orn"
                     /locus_tag="OHN27_001839"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCQ2286287.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18699..19751
                     /gene="rsgA"
                     /locus_tag="OHN27_001840"
     CDS             18699..19751
                     /gene="rsgA"
                     /locus_tag="OHN27_001840"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCQ2286288.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19848..20816
                     /gene="psd"
                     /locus_tag="OHN27_001841"
     CDS             19848..20816
                     /gene="psd"
                     /locus_tag="OHN27_001841"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCQ2286289.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20838..24161
                     /gene="mscM"
                     /locus_tag="OHN27_001842"
     CDS             20838..24161
                     /gene="mscM"
                     /locus_tag="OHN27_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCQ2286290.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24190..24504)
                     /locus_tag="OHN27_001843"
     CDS             complement(24190..24504)
                     /locus_tag="OHN27_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HCQ2286291.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24501..24815)
                     /locus_tag="OHN27_001844"
     CDS             complement(24501..24815)
                     /locus_tag="OHN27_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286292.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(24867..26369)
                     /gene="yjeM"
                     /locus_tag="OHN27_001845"
     CDS             complement(24867..26369)
                     /gene="yjeM"
                     /locus_tag="OHN27_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCQ2286293.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(26588..27565)
                     /gene="epmA"
                     /locus_tag="OHN27_001846"
     CDS             complement(26588..27565)
                     /gene="epmA"
                     /locus_tag="OHN27_001846"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCQ2286294.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIDALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27890..29698
                     /gene="frdA"
                     /locus_tag="OHN27_001847"
     CDS             27890..29698
                     /gene="frdA"
                     /locus_tag="OHN27_001847"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCQ2286295.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29691..30425
                     /gene="frdB"
                     /locus_tag="OHN27_001848"
     CDS             29691..30425
                     /gene="frdB"
                     /locus_tag="OHN27_001848"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCQ2286296.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            30436..30831
                     /gene="frdC"
                     /locus_tag="OHN27_001849"
     CDS             30436..30831
                     /gene="frdC"
                     /locus_tag="OHN27_001849"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCQ2286297.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30842..31201
                     /gene="frdD"
                     /locus_tag="OHN27_001850"
     CDS             30842..31201
                     /gene="frdD"
                     /locus_tag="OHN27_001850"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCQ2286298.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            31264..32397
                     /locus_tag="OHN27_001851"
     CDS             31264..32397
                     /locus_tag="OHN27_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCQ2286299.1"
                     /translation="MFKTTLCALLITASCSTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNALQ"
     gene            32486..33019
                     /gene="blc"
                     /locus_tag="OHN27_001852"
     CDS             32486..33019
                     /gene="blc"
                     /locus_tag="OHN27_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCQ2286300.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(33016..33333)
                     /gene="sugE"
                     /locus_tag="OHN27_001853"
     CDS             complement(33016..33333)
                     /gene="sugE"
                     /locus_tag="OHN27_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCQ2286301.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33515..33661)
                     /gene="ecnB"
                     /locus_tag="OHN27_001854"
     CDS             complement(33515..33661)
                     /gene="ecnB"
                     /locus_tag="OHN27_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCQ2286302.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33772..33897)
                     /gene="ecnA"
                     /locus_tag="OHN27_001855"
     CDS             complement(33772..33897)
                     /gene="ecnA"
                     /locus_tag="OHN27_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCQ2286303.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33949..34515)
                     /gene="efp"
                     /locus_tag="OHN27_001856"
     CDS             complement(33949..34515)
                     /gene="efp"
                     /locus_tag="OHN27_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCQ2286304.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34557..35585
                     /gene="epmB"
                     /locus_tag="OHN27_001857"
     CDS             34557..35585
                     /gene="epmB"
                     /locus_tag="OHN27_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCQ2286305.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8549 a         9836 c         9091 g         8391 t
ORIGIN      
        1 ttgcaatacg taattatctt accagctata gacaaaaaaa aaccatccaa atctggatgg
       61 cttttcataa ttctgagaaa ttagctgcgc tggcgcaccg cttcaaataa gcaaattccg
      121 gtcgcaaccg aaacgttcag ggaagaaacg cttccagcca tcgggatgct gatcaactca
      181 tcgcaatgtt cacgagtcag gcgacgcata ccttcacctt ccgcacccat caccagcgcc
      241 aggcgtccgg tcattttgct ctgatagagt gtatgatccg cctcgcctgc cgtaccgacg
      301 atccagatat tctcttcctg caacatacgc atggtgcgcg caaggttagt cacccgaatc
      361 agcggaacgc tttctgccgc gccgcaggct acttttttcg ccgtagcgtt gagctgtgcg
      421 gagcgatctt tcggcacaat caccgcatga acgcctgcgg cgtccgcact acgcaggcac
      481 gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca gcaggaacgg ttgatcgagc
      541 gaagcgatca gatccggcag atcgttttcc tgatactgac gtcctggctt cacgcgggcg
      601 ataatgccct gatgcacggc accgtcgctt ttctcgtcga gatattggcg gtttgccaac
      661 tggataacca cgccctgaga ctcaagggcg tgaatcagcg gtaacagacg tttatcttca
      721 cggcctttta aaataaagac ttcctgaaaa cgttcagggg cgcgctccag cagggcctgc
      781 actgcgtgga tgccgtaaat catttcgctc attaatgtac tcgttgttta cgttttgcct
      841 gatgcactac gtttatccgg cctacatgat ctctgcaata tattgaattt gcgtgattat
      901 gtaggccgga taaggcgttc acgccgcatc cggcatgaac aaagcgcatt ttgtcagcaa
      961 tctaaccctc ttcttttaaa gagggtattg atcactctgc cacttttttc ttcgccgcac
     1021 gcttcgcttt ggtcgctgca gctattttct gcgttttcgc cgatggcttt ttcgcttttc
     1081 tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc accgcggaag gcgctgtctg
     1141 gctcaaagtt taccttttta ccgacctgac gacgcttgcc gcctttttta cctacatcgc
     1201 cttttttcgc tttctcgcgc gccgttttac cgacgttgcg cggtgcgcgt tcgctggaga
     1261 tcaggctaaa gtcgattttg cgctcgtcca tattaaccgc ttcgacgcga acttccacgc
     1321 gatcgcccag gcgataagtc tggccgctgg attcccccat caggcgttgc cctacctggt
     1381 caaagcgata gtagtcattg tccagcgaag agacatggac cagaccatca atgaacaagt
     1441 cgtccagacg gacgaagaag ccaaagccag tgacgctgga aattacgcct ttaaagacgt
     1501 tacctacctg gtcgagcatg aagtcacact tcagccagtc agccacatcg cgcgttgctt
     1561 cgtcggcacg acgttccgcc atcgaacagt gctgacccag ttgcagcatc tcttccatcg
     1621 aataatggta gccgccggtt tcagtggtgt tgccctgatg cccctgctct ttcgccagca
     1681 gatatttaat ggcgcggtgc agcgtcaggt ctggataacg acgaatcggc gaagtaaagt
     1741 gcgcatagga ctgcaatgcc aggccaaagt gaccacggtt ttctggatcg taaatcgcct
     1801 gtttcatcga gcgcagcagc atggtttgca gcatttctgc atcaggacga tcggcaaccg
     1861 actccagcag ctccgcgtag tcacgcggtt ccggcttgtt accgcccggc agttccagcc
     1921 ccagctccgc cagcactgaa cggaaagagg taatcgcttc ggtgctcggc ttgtcgtgaa
     1981 tacggaacag tgccggttct ttcgctttct caacgaaacg cgccgccgag atattcgcca
     2041 gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg ctgggtctgt tcgatacgtt
     2101 caatacggcg ttcagcgttg aaaatgaact tcgcttcttc gctctcaaat gagatcccac
     2161 cgcgttcttc acgggcttta tccagcactt tatagaggtt atgcaactct tcgagatgct
     2221 taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc ctgcagaata tgccagactt
     2281 tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc gtagaatttg tagcccgtca
     2341 ggcggccttt cgacgaaacc gtcatctcgc acaccataca caggcggtct acctgcgggt
     2401 tgagcgaaca caggccgtta gagagcactt ccggcagcat cgggataacc tgcgaaggga
     2461 agtacaccga cgtgccacgg ttacgcgctt ctctgtccag cggcgttgac ggacgcacat
     2521 agtagctgac gtcggcaatc gcgacccata aacgccagcc gccgccgcgt tttttctcgc
     2581 agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc aatggtgacc agcggtaaat
     2641 cgcgcagatc aacacggccc gcttttgctt cttccggcac ttcttctttc agcccggcaa
     2701 cctgttgctc aacagcctgc ggccagatgt acggaatttc atgggtacgc agagcgatat
     2761 caaccgccat gccggtgccc atattgtcgc ccagcacttc gacgatttta cccaccgctt
     2821 tggtgcggcg agtcggacgc tgagtcagtt cgactacgac cacaaagccc atccgcgcgc
     2881 ccatgatctg atcgggcggg attaagatat cgaagctcag acggctgtcg tcaggaacca
     2941 caaagccgac gcccgcttcg gtaaagtagc gaccaacaat ctggctggtt tttggcacca
     3001 gtacgcggac aatacgcgct tcacgacgac ctttacggtc agcacccagc ggctgagcca
     3061 gcacctgatc gccatgaatg caggttttca tctgctcgct ggagagatac aaatcatctt
     3121 tacgcccttc aacccgcaga aagccgtagc catcacggtg gccaataacg gtacctttca
     3181 ccaggtcgag gcgttccggc agcgcatagc actgacgacg agtgaagacc agttgaccat
     3241 cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag ctgctcttcg ccttcaatgt
     3301 gcagttctac cgccagctca tcacggctgg ccggtttttc acgtttggtt aaatgttcga
     3361 ggataaattc ccgactaggg atgggattcg cgtatttttc agcttcgcgt tcctggaaag
     3421 gatcttgtga catctcggtt cctccgttgt catctctgat gaagattttc gtcactccac
     3481 cagcaataat ttataaagcg gttgattctc ttcaaccaaa tcggcaagcg tgtagttatc
     3541 cagttccgta agaaaacttt gcacggcctt agaaagtgcc tgtttcaacc tacaggcagg
     3601 tgtaatgtgg caaaactcac tgctgcaatt caccagcgat aagggctcca gctcgcgcac
     3661 cacatcacca atacgtatcg cactcgccgg tttacccagg cgaatgccgc cattttttcc
     3721 acgaacagca gtcacgtagc cggcacgact aagttgattg attattttga ccatatgatt
     3781 acgggagacg ccgtagacgt cagtcacttc agaaatactg gtcatccgcc cttctggcaa
     3841 tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg aaactcgtta actgcacatc
     3901 aacctcaaaa aggaaatcgg gaaaaaatac atttatattg atgataaacc agccacaagc
     3961 tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc gaggatttaa cactgtgtcg
     4021 gatagcgtaa tggcttaccc gacgcagcaa tgtatcacac tgttcgcccg gcaggcaaaa
     4081 tatctgccag gcgtaccaga attacgcgtc gaacgggtcg cgcagaatca tggtttcagt
     4141 acgatccgga ccggtagaga tgatatcgat cggcacacca gtcagctctt caatacgctt
     4201 gatatagttc agcgccgcct gcggcaggcc gctacgatct ttcacgccga aggtggattc
     4261 agaccagccc ggcatggttt cgtaaatcgg ctctacacct ttccagtcgt cagctgccag
     4321 cggagtggta gtcacttcgc gaccatccgg catacggtaa gccacgcaga gtttaacctc
     4381 tttcaggcca tccagaacgt ccagtttagt caggcagaag ccagacaggg agttcagctg
     4441 taccgcacga cgaacggcaa cggtgtccag ccagccggta cgacgacgac gccccgtagt
     4501 tgcgccgaat tcgttaccct gcttgcagag gaactcgcca gtttcatcaa acagttcggt
     4561 cgggaacgga cctgcaccta cacgagtgga gtaagctttg aggataccca gaacgtaatc
     4621 aacataacgc gggcccaggc cggaaccggt cgccacgcca ccagcagtgg tgttggaaga
     4681 agttacgtac ggataagtac cgtggtcgat atccagcagc gtaccctgcg caccttcaaa
     4741 catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg tcagaaacgt caaccaccat
     4801 agaagtcagg atgtcggcaa cagccatcgt atcatccaga actttctggt aatcaaccgc
     4861 ttcagctttg tagtagttaa ccaactggaa gttgtgatat tccatcactt ctttcagttt
     4921 ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc agaccgcgac gtgctacttt
     4981 atcttcataa gcaggcccga taccacgacc ggtggtgccg atcgctttcg cgccacgcgc
     5041 tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg atcagcggac atgcttcaga
     5101 cagcagcaga cgctcacgaa cggggatgcc acggtcttcc agttctttca tctctttcat
     5161 cagcgcggcc ggagacagca caacaccgtt accgatgatg ctggttacat tctcgcggag
     5221 aatacctgat ggaataagat ggagaacggt tttttcaccg ttgattacga gagtatggcc
     5281 tgcgttgtga ccgccctggt agcgtacaac atatttagcc cgttcagtca gaagatcgac
     5341 gatcttacct ttaccttcgt caccccattg ggtgcccagt acgacgacgt tgttacccat
     5401 ttttcaaaat caccgtttgc ttaaaaatgg attctaccat cgctttttca gagttacagc
     5461 actttttgca ctcaaaaatg acctcgatca gtctgttttt tgttcagcca atcgttttcc
     5521 tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc accgccaaaa cgacgtaaaa
     5581 tattatcggg caaattggtc atcgcagaga tcatcttctt ccatgccttc gggtaaagca
     5641 tcggccctaa accttccagt accaaaacca aggcaagcgc cagccagatt gtcgaattca
     5701 ttcattatcc ttatagaaaa agaaaaccac cgacatccct gaggatgcgg tggctttatt
     5761 gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt cggcgtcttc atgtagcgga
     5821 agaaatcgct atccgggctc atgaccatca cgtcctgatt gccagagaag ctgttctcat
     5881 aagcacgcag gctacggatg aatgcgtaga agtccggatc tttactgaat gcatcagcaa
     5941 acagtttggc tgcttcggca tcgccttcac cacgcatgat gcggccctga cgctcagctt
     6001 ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg cagtttttcc gcttcttcct
     6061 gaccttgtga acggtgacga cgcgctaccg cttcacgctc ggcgcgcata cggttgtaga
     6121 tcgcttcaga cacttcggtc ggcaggttga tctgcttgat acgcacatcg acaacttcaa
     6181 tacccagcgc cgccatactg ttcgggttga tgaccggaac tttgcccttc gtctcagccg
     6241 ttacgcgctc tgccgcttcg gcaatggcgt tatctgccgc cggggtagta acttcatctt
     6301 ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc gagggtcaga cgaccacggg
     6361 aatcggtgac gatatctttc acgtccaggc gaccaatttc agaacgcaga cggtcagaga
     6421 acttacgttt caacagcact tccgcttgcg aaatgtcgcc accacccgtt gccaggtagt
     6481 aacggctgaa atcgctgatg cgccatttga tgtaagagtc gacgatcagg tctttcttct
     6541 ctttggtcac aaagcggtcg gcctggttgt ccatggtctg aatacgtgcg tcgagcattt
     6601 tcaccgtttc aatgaacggt atcttgaaat gcagacccgg ctcataaacc agaggtttgt
     6661 tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat accgcgctca ccttctttga
     6721 cgacaaagac agacatgtaa agcactacca gcacgatgat gataatcgcg ataactgact
     6781 tacgcatcgt tattccccct gacgctggta gtcgttacgc tgcgcgttgg cgcggcgttg
     6841 gtccataata tcgccctgac tggtggacga cgtgttgctt gctccactgg ttgtggagga
     6901 agaggctggc ggcagacgca gcagattgct ggcaccgtta tcgctcttcg ccgcaggggc
     6961 gttaccacct ttcagcatct ggtctaacgg cagaaccatc aggttgccac ctttatcgtt
     7021 aaccagcact ttgcgggtgt tacccaacac tttttccatc gtctcgatat acagacgctc
     7081 gcgagtaatt tccggcgcgg ctttatattc cggcagaagt ttagcaaagc gcgccacttc
     7141 accctgagct tccaggatgg tctgggcctt gtacgcacgc gcctcttcga ggatacgttg
     7201 cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac gcttctgctt cacgaatgta
     7261 ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac gccgctttta cttcttccgg
     7321 cggacgagca gcctggaagt tgacgtccag cagcgtgata cccatgtcat acggacgaat
     7381 cgtctcttcc agttcgcgct gagtatcgct acgaatcacg gtacgacctt ccgtcagaat
     7441 gcggtccatg gtgtatttac cgataactcc acgcagggcg ctgtcggtag cctgacgcag
     7501 gctgtcatcc gggctggtca cgctatacag atatttttcc ggattggtga cgcggtactg
     7561 cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc agcatcacac cagaagcggc
     7621 cagttcacgc acggcttcca cgttcaccgg tttgacttcg tcgataaacg tcggtttcca
     7681 gttcagaccc ggctcaacca gatggctgaa tttaccaaag cgtgttacca cgccgcgttc
     7741 ggcttcttta atggtataga aaccactggc cgcccagata atgacaatcg ctgccgctgc
     7801 gatggtaacg acacgaccgc caagctgcgg gcgcgggcct tgcgatgaac tgccaccgcc
     7861 agatccggtg cctttaccgc cgcccagacc accgagcttt ttgctcagtt tgcggaagat
     7921 atcatctaaa tcaggtggcc cttgatcgcg accgcctttg tttccatttc cctcagagtt
     7981 gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt ccgttattac cgggctgatt
     8041 ccacgccatg tttgtgctcc atatttgtta tgcggtgatc cctgttgtct ttcgacgcca
     8101 gaggatatga ctccacgctt cagacgctac gccgttagat caggtaatcg atcaacgccg
     8161 gttcttgttt acagaggcga cgccagtcaa cgatcggcat acgaacttgc agacttacgc
     8221 tgccgtcctc ctccatccac tctttttcta ttgcctgaag ctgataaaaa cgacttctca
     8281 gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc cacctcgccg gaaagccgct
     8341 ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt ctgtgcggaa agccagacac
     8401 ggatcggttt gttctcttcg tcccgatcaa tacgcggttc gaaatcttcc agcatatcga
     8461 tcttgttcat caccagcagg gttgggatct cgtgagcgtc gatctcttca agaaccgtat
     8521 tcaccgcttc gatgttttct tgtacacgca catccgccgc atcaatgacg tgcagcagta
     8581 atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc cgccaccaga tcgtgcggca
     8641 ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc accgacatct gcaacgtcaa
     8701 tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc tgccgcgtag acccgcgctt
     8761 cggtgatgcg attgaaaagg gtagatttac cggcgttggt atatcccacc agcgaaacag
     8821 taggaacgtc ggctttgata cgcgattgcc gcccctgctc acgctgcttt tcaactcttt
     8881 ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa acgacggtcg gtttcgagct
     8941 gggtttcacc cggaccacgc aaacctatcc cgcctttctg tctttcaagg tgggtccagc
     9001 cacgcaccag gcgcgtagcc agatggcgca gctgcgccag ctcaacctgc aacttaccct
     9061 catgggtacg cgcacgttgg gcgaaaatat ctaaaataag gccggtgcgg tcgataacac
     9121 gacactcgca caaacgctcc aggttacgct cttgcgccgg gctcagggca tggtcaaaaa
     9181 gaacgaccga agcacccgtc gctttgacag cttccgcaat ttcaactgct ttaccttcac
     9241 ctacaaaata ctttgggtgc ggcgctttac ggctaccggt aatcacctgc aatgcttcga
     9301 caccggcgga agagaccaga gattcaaact cctggaggtc ttccatatct ttgtcttgcg
     9361 taaaatagat gtgtaccagt accgcctgct caccagcatc ataacggtca aacaagcgta
     9421 taaccctcta aatagatcag cggggaacgc aggatcgctg gctccccgtg taaaaaaaca
     9481 gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc ggaagtattc tgcgcgctgc
     9541 taccatgatg gtagttactg ctggtaccgc caccggcgtt gttactgtga tgagaaaccg
     9601 ggcgagacgg gacaacagta gaaatcgcgt gcttgtaaac catctggctg accgtgtttt
     9661 tcaacaggat cacgaactga tcaaaagact cgatttgccc ttgcagctta ataccattca
     9721 ccaaataaat agaaactgga acacgttccc gacgcagtgc gttcaggaac ggatcttgta
     9781 aagattgccc cttagccatt ctctcttttc cttatatgct tatttgtact ttgaaccttt
     9841 cgattctgaa aaaattgcgc acgatacgtc tcaattgtac acattcagcc tgcgatagca
     9901 ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt tttcactgtc aagccagtga
     9961 accccttccc aaccacgcag ccaggttatc tgccgcttcg ccaactgtct cgtggcgcaa
    10021 acacctcgat aaaccatttc atcgtatgag atttcgcctt caaggtaaga ccacatctgg
    10081 cgataaccca cgcaacgaat ggaaggcaag tccgtatgca aatctcctcg ggcaaaaagc
    10141 gcccggactt ctgcttcaaa acctgaagcc aacatctgat gaaaacgctg ctcaatgcgt
    10201 tgatggagca gttcacggct cgccggggcg atggcgaact gatgcacctg atacggtaga
    10261 gcgtctcctg acgtttgcgt cagttccgtt aaagttttac ccgaaatgaa aaaaacttcc
    10321 agtgcccggg aaagcctttg tggatcattt ggatgaatcc ttgctgccgc aaccggatct
    10381 acctcctgaa gttgacgatg caatgactcc caaccttgct ctgccgcctg ttgctcaatt
    10441 ctggcccgta cttccgggtc tgccgacggt agcggcgaca acccttccag caatgccttg
    10501 aaatacaaca tcgtaccgcc cactaacagt gggatccgcc ccgccgcggt gatatcggcc
    10561 atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt aagcctgcga cggatcgcga
    10621 atatccagca atcggtgcgg cgcggcgagt aactcttcag cgttcggctt cgccgtcccg
    10681 atatccatcc ctttgtaaat aagggcagaa tcaacgctta tcaactctac tggtaaaatt
    10741 ttacgcagct caatggctaa cgccgtttta ccggaggccg tcggccccat caaaaaaatc
    10801 gccttaggca ggctcgcctt actgatatca ctcatctttc agggctttta tcgccggatg
    10861 taaatcaaca gattgtaaca gaccacccgg cggcgttttc acaagttgcg gacataaccg
    10921 ttccacgtcc gccagcaggg ttatggcctg tgccattgac cactgcgcat gttcgctcat
    10981 cagatttcgt gcaatccact gcgcaatatt gccaggttcg aatacggact gcttcgccag
    11041 gtagcctatc agttcaggaa tcaagatttg taaattttgt tggcgtaagg gtaaaggcac
    11101 tgccctgatg gtcacatgct gtgcatctga ctggaaatca atacccaatt ccgccagggc
    11161 agactgcgct ttttctaatg ccgatttttc ttcggcagaa acttttagcc gcaacggaat
    11221 cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc aattgtgcct gacgcagcca
    11281 acgttctgcc actggcaagg ataaaagtga aatgttgccg tcgcgctcca gcaacgcaca
    11341 gtcggaatgg acgatagtca gtacccgacc aaaactctga ctgttcgccg caagtgcagg
    11401 ttcctgcggt tccggcgctt ttaatttttg catcggcgcg ggcgtttgca aaagctggcg
    11461 atacacttca ccttgctgtt tctggtagcc tggctgcgca ttcggccagg gggcagccgg
    11521 acgactgcct gatgctggcg caggagtgta gcgcggagct accggctcac gagctgccgg
    11581 ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc ggaatggaac gcggtgcagg
    11641 ttggggttca tcgtccagcg gtagcggcgt ttccagttgc tgttgtagca cgctcagcac
    11701 gccctgatag ataaaatcat gcaccagacg cgactgatgg aaacgcactt cgtgtttggc
    11761 ggggtgcacg ttgacgtcca cctgatgcgg atcgatctcc agatacagca caaatgccgg
    11821 ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg atcgcgtgat tgatcaggcg
    11881 atcgcgcatc atgcgaccgt tcacgtagca atactgaatt tctgccagtg cgggcgtggt
    11941 gtgatttgga tcggccaccc agccgcgtag cgtgagatcg ccgtgttgcc attcaatcgc
    12001 cagcgcttgt tcaagaaaag cggtgccgca aatcgcgcct aagcgccgtt ctttttgccc
    12061 gccttccggc actgcgcggt actgacgcac aattttaccg ttatgcgaca ggttgatcgt
    12121 gacgtcgaaa cgcgccagcg caatgcggcg gatgatctca tcaatgtggt taaattcggt
    12181 tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg tagaacagat ccagcacctc
    12241 cagcgtcgtc cccacaggat gcgccgccgg ttttaccgtc acgttcatat cgcgcccttc
    12301 ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt gaagtgagcg tcaggcggga
    12361 aaccgaactg atactcgcca gcgcctcacc gcgaaagccc aggctgataa tggcttcgag
    12421 atcgtccaga gaggcgattt tactggtggc atgacgagcc agcgccagcg ccagctcatc
    12481 ttttttgata ccgcagccgt tatcacgaat gcggataagt ttcgccccac cgcgttcgat
    12541 atcaatatcg atacgcgtcg cacctgcatc gaggctgttt tccactagtt ctttgactac
    12601 cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg ttcgccagtt gtggcggtaa
    12661 gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg cgatctggcg tcgtcaccgt
    12721 actggcagtt tgtgccgttg caccctgcgg cgcagattgc atcggatgcg caaggaagta
    12781 attgcgcagg cctttgtaaa tggcttctgc cagctgttgt tggtaatcgt cgctcgccag
    12841 caaacgttct tcgctgttgt tgctgataaa accggtttcg accagtactg atgggatatc
    12901 cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga cgtttatgta tttcgccaat
    12961 gcgttgcaac tgactgatca tactggtcgc tacatcatac cctacccgct gggaatgacc
    13021 gaactgtaaa tccagcaccg cctggcttaa ataggggtca gactgactgt tcgccagcac
    13081 atcacccgcc ccacccagca gctccgactg tttctcgtgc tgctccagcc agctggccat
    13141 ttcactgttg gcgcgacggt tagagagcac ccatacggaa gcgccagtcg cactgcggtt
    13201 cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg ttttgcttac gtgccacatc
    13261 actgcgcccc atcaccgaga taaagtaatc cccgtcacgg gttaaaacgc ctttaaacat
    13321 cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg gcgatggtga catttttctc
    13381 ccgcgtacca ccggggccga tagcgccagg gtcctgaccg ccgtgtccgg catcaatagc
    13441 gatgataatt ttatcgccag tgttagccgt cgcgcgcgct gccggacgcg ttaccgtatt
    13501 actgctgata acacccgtag tgcggttact ttccgtttta aacggattgc gcgccggttc
    13561 gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt ttcgcaacca cgggcggcgg
    13621 aggaggcggt ggcggcacat cggcgttaat cgtaaagacg acagtgtaat tgctgccatt
    13681 ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga tcgaccacca gccgtagcgt
    13741 ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc agattattgc cgctgaacaa
    13801 caacggcagt ccctgaatca cgcccgtttg tttgatatcg agcgccacgg tgcgtttgct
    13861 ttgatggcta aacgcataat caggatcgcc aataaaactc aacgttatcc gcgcctgttg
    13921 attaccgtta gaaacctgaa tatcagagag cgtcgcggca cccaccggcg tgcacagcag
    13981 cagcagcgtc gctaccaacc aatttctgat gcgatacatc atcccgccac ctttcaaagg
    14041 ttaaccggct aaacgcgcca gcaacaattc acccgcagag gaaaccgcac tcacgcgcgc
    14101 ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca tccgggtcag gaagaacacc
    14161 tgtaccttgt tgtggccact ccaccaggca gatggcatcg ttggcaaaat aatcgcggat
    14221 ccccataaac tccagctcct cgggatcggc aaggcggtac aaatcaaagt gatagaccat
    14281 taagttgtcg agcgtatagg gttcgaccag cgtataagtg gggcttttga cattaccctg
    14341 atgacccaga gcctgtaaaa agccccggct aaaggtggtt ttacctgcgc ctaaatcgcc
    14401 atacagatag attacggttg cgccatcgca ggctttcgct acccgctcgc ccaggtctaa
    14461 tgttgcctgc tcatcaggga gcggaattac tcgattcatc atggttttta tcagtcactt
    14521 ccgggttaac aatacgctgt agcgtggaaa agagatcggt tgccagcatc ccgcgcgttc
    14581 caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc gacacagcct gcacaggctg
    14641 catcatacgg cgacagtttt tgcccaagca atgcgccaat aataccagag agcacatcgc
    14701 ccatgccgcc gctcgccatg cctgcatttc cggcatcaat aatgcctaaa gcgtcaggat
    14761 gggcggcgac cacggttccg gcacctttca gcaccgctac gccgccataa cgttgtacca
    14821 gacgtttggc gcaatgtaag cggtcacttt caatttcagc gacggaacag cctaacaacc
    14881 gtgcggcctc gccaggatgc ggcgtgatca cgcgattgtg acgcttatcg ggattgattg
    14941 ccagcaggtt caatgcatcg gcatcccaca acatcggttt gcgaaaattc tcaacttttt
    15001 gcagtgcttt tttcccccac tcttgctggc ccagaccggg accaatcacc accacatcgg
    15061 cccattccag gctttcggta agagagtcca tcgtcagttc atgcaccatc aattccggtc
    15121 gtgcagtcag cagcggcgca atgttttcac tgcgggtcag tactcggact aaaccagcac
    15181 cagcacgcag cgccgcttcc cccgtcatac gaatagcccc cgccgtgccg tgatcgccac
    15241 cgataattac cagccgcccg tgatcgcctt tatgcgaagt cgggcgacgc ggttttagcc
    15301 agtgagaaag ttgttctgct gaaaaccgct gaattttcgt ctcctgacct gccagccaac
    15361 tatccagccc cagtgagtca aaatgcagtt gtccggtaac atcccgcgct tttccagtga
    15421 gcaagcctgg tttcagcgca ataaaagtga tggtgtgatc ggcgttgatc accgcgcctg
    15481 gcgtagcgcc agtttcagcc agcaggccgg aagggatatc aaccgccaca atcggcgcag
    15541 gatgggaatt agcgtggtcg attaactggc taatggattc gcggggcgct tgccgcaaac
    15601 cggtaccgag cagcgcatca acaatcagat ctaccgattc gggccagaca atattcgaag
    15661 catggatctc gccacccgcg tttaaccatg cttcgcgtgc cagcgcggcc tcttccggca
    15721 acggtttgtc gctctcctgg gccaacaacg tgacctcaat gccgaccgct ttggccagtc
    15781 gcgcgaccac gtagccatcg ccgccgttat taccatgacc gcacagcacc agccagtggc
    15841 gggcgtcagg atacgccgaa cgacacacct ggaatgcggc ctcgccagcg cgaagcatca
    15901 gctcatagag tgtgagcccc agcacatctg ccgcctcgcg ttctccgcgg cggatatcgt
    15961 cggcgtacca gacggtgtgt ggtatactta cggggttttt cttcattgta tggtccgtca
    16021 tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat taaacagtgg gggctggaac
    16081 tgggctttca gcaggtaggt attaccgata ccgatctcag cgagtccgag cccaaactgc
    16141 aagcatggct ggacaaacaa taccacggcg aaatggactg gatggcacgt cacggtatgc
    16201 tgcgcgctcg ccctcatgag ttattgcccg gtacgctgcg cgtgatcagc gtgcggatga
    16261 attaccttcc tgctaacgcc gcatttgcca gcacgctgaa aaaccccaaa ctcggctatg
    16321 ttagccgtta tgcgctgggc cgtgactatc acaaacttct gcgcaaccga ctcaaaaagc
    16381 tgggcgagat gattcagcaa cattgtgttt cgctgaattt tagaccgttt gtcgattctg
    16441 cgcctattct cgagcgcccg ttagctgaaa aagctgggct cggctggaca ggtaagcact
    16501 cacttatcct caatcgcgag gccggttcgt tcttcttttt aggcgaattg ctggtcgata
    16561 ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg caaatgcgtg gcctgtatga
    16621 cgatttgccc gaccggtgcc atcgtcgagc catataccgt cgatgctcgc cgctgtatct
    16681 cttatctcac catcgaactt gaaggggcga tcccggaaga gttgcgaccg ttaatgggaa
    16741 accgtattta cggttgcgat gactgccagc ttatctgccc gtggaatcgc tattcacaac
    16801 tcaccacaga agaggatttc agcccgcgta agccgctaca cgcaccggaa ctcattgagt
    16861 tattcgcctg gagcgaagag aagtttttaa aagtcacgga aggatcggcg attcgtcgta
    16921 ttggtcacct gcgttggctg cgtaatatcg ccgtagcatt aggcaatgcc ccttgggatg
    16981 aaacgatttt gacagcgctg gaaagtcgta aaggtgagca cccacttctc gatgagcaca
    17041 tagcgtgggc gattgcgcag caaatagaga gacgaaatgc gtgcatagtc gaagtgcaat
    17101 tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg gttaccgcgt gacgcctgat
    17161 tcatccacag cttgtgaata aaaataaaaa cgcattgcaa ttcaagacga ataaaatcgt
    17221 caagtgatcg actcgacaaa acggaatctt aatttttaac ttatctatca ataggttaaa
    17281 aaaatactat tcaccgtgcg aagtttcttt gaaattaaga ttacagcctg aggctgtgga
    17341 taactctgtt tacaagagtt tgtcagagac aacgaaaacc agcaataacg catcgcatcg
    17401 ctgtggatat tttattgaga gaagaatttg gagcgggaaa cgagactcga actcgcgacc
    17461 ccgaccttgg caaggtcgtg ctctaccaac tgagctattc ccgcttgggt ggtctgtgct
    17521 ttacagcact ttcaaatttt ggagcgggaa acgagactcg aactcgcgac cccgaccttg
    17581 gcaaggtcgt gctctaccaa ctgagctatt cccgcttaat cttcgtcttt cgaaccgcta
    17641 ctgccttggc tgccttcact caccttagtc acatactttt gtaagttccc aaggattcgt
    17701 tcagttgccg ccttgtcgca attcgaaata ctgcgattgg gtggtctgtg ctttgcagca
    17761 cttttcaaat tttggagcgg gaaacgagac tcgaactcgc gaccccgacc ttggcaaggt
    17821 cgtgctctac caactgagct attcccgcgt actacttaat tttgctttcg taattttcgc
    17881 atttctgcgt cgttacggga ggcgcattat acgagaaatc tttccctctg caacccccca
    17941 aaaaagtttt ttttgcgttt ttatctcaag tgctgaaatt atcagcaaga cgttcaattt
    18001 agcgtcaaaa tcacgccgcc gcaagtggat tccggcgctt acgtgaccag gaaaaatttt
    18061 acagcttgat aaaatgctcg cggtagtaag ccagctccgc caccgattca cggatatcat
    18121 ccatcgcctg atgcgtcccc tgcttggtaa aaccatccag aatttccggc ttccagcggc
    18181 gcgccagctc tttcagggtg ctgacatcga gataacggta gtggaagtag gcttccagct
    18241 ccggcatgta tttaaacagg aaacgacggt cctgaccgat gctgttaccg caaatcggcg
    18301 attttcccgc aggcacccac tgttttaaaa attcgagcgt tgccagttca gcttcccgat
    18361 cgcccatcgt gctcgctttc acgcgctcta ccagcccgct ggcggtatgg gtgcgcacgt
    18421 tccagtcatc catcagcgcc agctgttcat cagactggtg tactgcaatg gtcggccctt
    18481 ctgccagaat attcaggttg gcatcggtca ccagcgtggc aatctcaata atgcgatcgc
    18541 gctcgggatc cagaccggtc atctcaagat cgatccaaat caggttgttt tcattggcac
    18601 tcatgctatt ttccacccat tcacagtgac tatattgatc cacaattgcg tgtatcatag
    18661 atgttttgcc catcaggggc gaccaggagt ctgtacgatt gagtaaaaat aaactctcca
    18721 aaggccagca gcgccgcgtg aacgccaatc accagcgtcg tcttaaaacg tctaaggaga
    18781 agcccgacta cgacgacaat ctgtttggcg agcctgatga aggtatcgtc atcagccgct
    18841 ttggtatgca cgctgatgtg gaatccgccg atggcgacgt tcaccgctgc aatattcgcc
    18901 gtaccatccg ttcgctggta accggcgacc gcgtagtctg gcgtccgggt aaaccggcgg
    18961 cggaaggcgt aaatgtcaaa gggatcgtgg aagcggtgca tgagcgtacc tcggtgttga
    19021 cgcgtccgga tttctacgac ggcgtaaaac ctattgccgc caacatcgac cagattgtca
    19081 ttgtctccgc cattttgccg gagctgtcgc tcaatattat cgaccgttac ctggtggcct
    19141 gcgaaacctt gcagattgag ccgattattg tgctcaacaa gatagacctg ctggacgacg
    19201 aaggcatggc gttcgtcaac gagcagatgg atatctaccg caatatcggt tatcgcgtat
    19261 tgatggtttc cagccatact caggatgggc taaaaccgct ggaagaggcg ttgaccgggc
    19321 gcatcagcat ttttgccggg cagtctggcg tcggcaaatc cagcctgctg aatgcgttgc
    19381 tggggctgca aaaagagatc ctgaccaacg atgtctctga caactcgggg ctcggtcagc
    19441 acaccacgac cgccgctcgg ctgtatcact tcccgcacgg cggtgatgtg attgactccc
    19501 caggagtgcg tgagttcggc ctctggcacc tggagccgga acaaatcact cagggctttg
    19561 tcgaattcca tgactattta ggtctgtgta aatatcgcga ttgcaaacac gataccgatc
    19621 cgggctgcgc tatccgggaa gcggttgagg aagggaaaat cgcggaaacc cgtttcgaaa
    19681 actatcaccg tattctggaa agcatggcgc aggtaaaaac gcgtaaaaac ttttctgata
    19741 cggatgactg acaattaagc taaccgtcgt tagaatcgtc cccttttttt caggatccgg
    19801 catgtaggcc ggatcaggaa cgacaaaaca atggcctgga ggctaccttg ttaaattcat
    19861 ttaaactttc gctacagtac attctgccga aactatggct tactcgcctg gcgggttggg
    19921 gcgcaagcaa gcgggcagga tggctgacaa aactggttat cgatctgttc gttaaatact
    19981 acaaggtcga catgaaagag gcgcaaaagc cggacaccgc cagctaccgc acctttaacg
    20041 aattctttgt ccgtccgctg cgtgacgaag tacgcccaat cgataccgat ccgaatgtac
    20101 tggtcatgcc tgccgatggc gttatcagcc agttgggtaa aatcgaagaa gataaaatcc
    20161 tgcaagccaa aggccacaac tacagcctcg aagccctgct ggcaggcaac tatctgatgg
    20221 cggacctgtt ccgcaacggt acgtttgtga ccacttacct ctccccacgt gactaccacc
    20281 gcgtacacat gccgtgcaac ggtattctgc gtgagatgat ctacgtgccg ggcgatctct
    20341 tctccgttaa ccatctcacg gctcagaacg tgccgaatct gtttgcccgt aacgaacgcg
    20401 tgatttgcct tttcgatacc gaatttggcc caatggcgca gattctggtc ggagcgacga
    20461 ttgttggcag cattgagacg gtctgggcgg gcaccattac gccgccgcgc gaaggtatca
    20521 tcaagcgctg gacctggcct gccggggaaa acgacggttc tgtggcactg ctgaaaggcc
    20581 aggaaatggg tcgctttaaa ctcggttcca ccgttatcaa cctgtttgca ccgggtaaag
    20641 tgaatctggt tgagcaactg gaaagcctgt ctgtcacgaa aattggccag ccgctggcag
    20701 tatctaccga aacctttgtt acgccagacg ctgaacctgc cccgcttcct gctgaagaga
    20761 tcgaagcaga acacgacgcc agcccattgg ttgacgacaa aaaagaccag gtctaatcca
    20821 tcaaaggaaa cgctgacgtg cgcctgatta tcacttttct gatggcctgg tgcctcagtt
    20881 ggggggcgta cgccgcgacg gcccccgata gcaaacaaat cactcaggaa ctggagcagg
    20941 caaaagcggc gaaacccgca cagccggaag tcgtagaggc gctccagtct gccttaaatg
    21001 cgcttgagga acgaaaaggt tcccttgagc gcatcaaaca atatcagcaa gttatcgata
    21061 attatccgaa actctccgct actctgcgcg cacaattaaa caacatgcgt gacgagccgc
    21121 gcagcgtgtc gccgggaatg tctaccgacg cgctgaatca ggaaattctc caggtcagca
    21181 gccagttgct ggataaaagc cgtcaggccc agcaagagca ggagcgcgcc cgcgagattg
    21241 ccgattcgct gaatcaactg ccgcaacagc aaaccgacgc ccgccgtcag ttaaatgaga
    21301 tcgagcgccg cctgggaacg cttaccggca atactccgct caatcaggca caaaatttcg
    21361 cgttgcagtc tgactctgca cgtcttaagg cgctcgttga tgaactggag ctggcgcagc
    21421 tgtctgccaa taaccgccag gaattagcgc gcttacgctc agagctggcg gaaaaagaga
    21481 gccagcaact ggatgcgtat ttgcaggcct tgcgtaatca attaaacagc caacgtcagc
    21541 ttgaggcgga gcgggcgctg gaaagtaccg aattgctggc agaaaacagc gccgatttgc
    21601 cgaaagatat cgtcgcgcaa ttcaaaatta accgcgaact atcggcggct ttgaatcaac
    21661 aggcgcagcg gatggatctc gttgcctcgc aacagcgtca ggctgccagc cagacgttac
    21721 aggtccggca ggcgttgaat acgctgcgtg aacagtcgca atggctggga tcgtccaatc
    21781 tgctcggcga agcgctgcgg gcgcaggtgg cacggctgcc ggaaatgccg aaaccacaac
    21841 agcttgatac cgaaatggcg cagttgcgtg tgcaacggtt acgttatgag gatctgctta
    21901 ataaacagcc gctgctacgg caaattcatc aggccgacgg tcagccgctg actgccgagc
    21961 aaaaccgtat tctggaagca caactgcgca ctcagcgtga gttgctgaac tcattgttgc
    22021 agggtggcga cacgctacta ttggaactga ccaagctgaa agtctccaac gggcaactgg
    22081 aggatgcgct gaaagaggtg aacgaagcaa cgcaccgcta tctgttctgg acctctgacg
    22141 tgcgcccgat gaccatcgcc tggccgctgg aaatcgccca ggatctgcgt cgtctcattt
    22201 cgctggacac cttcagtcag ttgggcaaag ccagtgtgat gatgctgacc agcaaagaga
    22261 caattttgcc gctgtttggc gcgttgattc tggtcggttg cagtatttac tcgcgccgct
    22321 atttcacccg ttttcttgaa cgttcggcgg cgaaagttgg caaagtgact caggatcact
    22381 tctggctgac gttgcgcact cttttctggt cgattctcgt cgcgtcaccg ttaccggtgc
    22441 tgtggatgac gctgggttac ggcttgcgcg aggcgtggcc ttatccgctg gcggtcgcga
    22501 ttggcgatgg tgtaacggcc accgtgccgc tgctgtgggt agtgatgatt tgcgccacct
    22561 ttgcccgccc gaacggcttg tttatcgctc attttggctg gccgcgcgaa cgtgtttccc
    22621 gtgggatgcg ctactacctg atgagcatcg ggcttattgt gccgctgatt atggcgctga
    22681 tgatgttcga taacctcgac gaccgtgaat tctccggttc gctgggacgg ctttgcttta
    22741 tcctcatttg cggtgcgctg gcggtggtca ccctcagcct gaaaaaggcc gggatcccac
    22801 tgtatctcaa caaagagggc agcggcgaca acattaccaa ccatatgctg tggaacatga
    22861 tgattggcgc gccgttggtt gccattctgg cgtcggctgt gggttatctg gcaacggcac
    22921 aggcgctgtt agcgaggctt gaaacctcgg ttgccatctg gttcctgcta ctggtggttt
    22981 atcacgttat ccgccgctgg atgctgatcc agcgtcgcag gctggcgttt gaccgggcga
    23041 agcatcgccg ggcagagatg ttagcgcaac gcgcgcgtgg cgaagaggaa gcgcatcatc
    23101 acagtagccc ggaaggggca attgaagtcg atgaaagcga agtcgatctc gatgccatca
    23161 gtgcgcaatc cttgcggctg gtgcgctcaa ttttgatgtt gatcgccctg ctttcggtca
    23221 ttgtgctgtg gtcagaaatc cattccgctt tcggcttcct cgaaaatatt tctctgtggg
    23281 atgtcacttc cactgtacag ggcgtagaaa gcctggagcc gattaccctc ggtgcagtgc
    23341 taattgccat tctggtattt atcatcacca cgcagctggt gcgcaaccta cccgcgctgc
    23401 tggaactggc gattttgcag cacctggatt taacgccggg tacgggttac gccatcacca
    23461 ccatcaccaa atatctgctg atgctgattg gcgggctggt cggcttctca atgattggta
    23521 ttgagtggtc gaaattgcag tggctggttg ccgcgctcgg tgttggtctc ggttttggtt
    23581 tgcaggaaat tttcgccaac tttatctctg gtctgattat cctgttcgaa aaaccgattc
    23641 gcattggcga tacggtgaca attcgcgatc tcaccggtag cgtgacgaaa attaacaccc
    23701 gcgccaccac catcagcgac tgggaccgta aagagataat cgtgccgaac aaggcgttta
    23761 ttaccgagca gtttatcaac tggtcgctct ctgactcggt cacgcgcgtg gtgttgacga
    23821 taccggcccc tgccgatgcc aatagcgaag aagtgacgga aatcctgctc accgcagcgc
    23881 gtcgctgctc gctggtgatc gacaacccgg caccggaagt cttcctggtg gatctgcaac
    23941 aggggattca gattttcgag ctgcgtattt acgccgctga gatgggtcac cgtatgccgc
    24001 tacgccatga gatccaccag ctgattctgg ctggcttcca tgcccacggt atcgatatgc
    24061 cattcccgcc cttccagatg cgtctggaaa gcctcaacgg taaacaaacg gggagaacgc
    24121 tgacgtctgc gggcaaaggt cgtcaggcgg gaagtttgta agtttgtgtg ccggatgaac
    24181 caatccggct cacaaacaaa actgatagcg cagaaaaaac tgccacagcc agtagccgaa
    24241 aatagccagc gcggtggcgt acataaacca gccgcgtttt tttcgccaca aagcgaacca
    24301 gagaaaaggg aaaaagagcg gtaacgtcat tatcacacca acatcacggg tatcgtcttc
    24361 ttcgagatac atcaacacat cgcagacgtt gttaatcccg cgtccatcaa tcatccactc
    24421 tttgtccagc gcggaagtga tggttatcca gaggaacgcc acaataaacc agatgcagca
    24481 taagacaaac atgcgcgcac tcataaggta accggtcgct taatttcgct atagcgcata
    24541 cgaaacaaat cgccgtatcc cactaattcc ccgcgaaaaa ccagacatcg gataagataa
    24601 gcgcaataaa tatccggcag gtttccccac cgtttaccat cgcgatcttt gagggtgtta
    24661 atggtgctac ccaccaccat cgccacttta cgggtgtggc aggcatcgac aaaagagaga
    24721 atcgtctgct ctatttccgc tacatccccg gcagaaaatt gcagcgccct gatttcatct
    24781 tcggttattg ccgggtcccg gctggtgtca tccataacat ttcctgtctt tacgccctat
    24841 cgtcaggtag acgatagggc atcgggttat tccaccgccc actgcggatt ttttgccact
    24901 ctgctgcaat agttctggta aatcgccatc gctaacagcg agaagaagac agggccgcca
    24961 atcatccaca atgtgctgtc ccagtctcca gcttcaacca caggttgaat gatggtgaag
    25021 acgttcgcaa atgtcaccac cagtacgaca accaccgtcg cgatcattgc cgacatacgc
    25081 gttttgaaaa tcacaaatgg tctgtcgaga tcctgacgtg ctttaaagaa tgggaaagcc
    25141 agcgcgagga acaggtaagg aagcgtcata gacacgttcg ccatcagcgt cagcttgtta
    25201 aagaacgccg atgcggtacc gccaccaaac gaaaccagca gaatgaagat agtaaccaac
    25261 ccgcactgca tccacatggc gatagacggc atccccatcg cattcaggcg cgtcatcggt
    25321 tccggccaca atgctttcgg cgtcccctgg atgatggctt tcaacggtga atagcaaagc
    25381 gtaaagaacg caccggtata ggcgaggaac atcgaaagtc cagtaatacg cgcaaaccat
    25441 acgcccagcg acaatgacgc ttcaggtgac aaatgcagtg cattacccag cgtcatccca
    25501 aggctcttca tcagcacata ggtaatattg ccgaggttaa cggaaccatt acttaatacc
    25561 tgctgccagt ttgtgctgac gccccataaa aatattgcca gcgaataacc gattgaaata
    25621 acaatagcgg caaaaacaat acctttggca aagttctttt ctggattttc cgttttatcg
    25681 accagaccac caaccgcttc aattccgcca taggcaaaaa tggcaaatac cacaaacgat
    25741 agcattgcca gaccggactg ataacccggg ttcggtgatg caaggaaatt aatatcctgc
    25801 gcgaaatgcc caccatttaa taacaaaata gtaatgctta ctaacagcaa tactaaattc
    25861 agacacatta ctgcaatacc gcccaccgca gtaatgcggg caattttatt aatcccctta
    25921 gaagcaacga cggtgaccag aatcatccat gccactgcca gcagaccaac cacctgcgta
    25981 ggctccagtc cggcaatacg ccagtgctgg gtcatgtcgc taccatagag gaatgttgag
    26041 aacggtaccc aaactttcgc ggaggtactc accatccaga tgatataaga ggaaaaccac
    26101 ataaacgtac caatgaaggc aaaacgtggt ccgacactat tattcatcca ggaatagata
    26161 ccaccttctt ctttgcgata agcagctccc atttcagcca tcattaaggc gaatggaata
    26221 aagaataaca atgcagaaaa tatataaaag ggaatcgcac tataacccat taagtaataa
    26281 gccgatgggc tattggcaaa accaaatacg gaagtaaaga tcatcaatat gagtcctatc
    26341 agactcattt tttttatcgt gtgaggcatg aaaccatcct tcatttcagc gcgtgtataa
    26401 cacgtgccca ctgaactgca ctgcgcaaca ttgagcaggc agcgtaaaaa ccaatacaga
    26461 attgatacct ggatgatatc aaatatccag tcttaaattg tggcctttag cggaaaaatg
    26521 aaaacatatg ctacagaatg gcgcttatta cgccattctt cgctgttaat tcagtaattt
    26581 ttcagaatta tgcccggtca acgctaaagg cgatgacttc agccagtgtc tccgcgccca
    26641 gcgccaacat caccagacga tcaacaccta atgccacgcc ggaacagtca ggcataccga
    26701 ctttcaaggc gtcaatcaga ttctggtcaa tggggtgctg cggcaaaccg cgcgccgcgc
    26761 gcttacggtt atcttgttca aagcgttgtt gctgctcacg ggcatccgtc aattcatgga
    26821 aaccattcgc cagctcaata cctttataat aaacctcaaa gcgttcagcg acccgatgat
    26881 cttcggtact gatttgcgcc agtgatgcct ggctggctgg aaagtggtac acaaaggtcg
    26941 gtttttcttt accaatattt ggctctacgc caaaggtaaa cagcaattgt agcaacgtgt
    27001 cgcggtcttc ttcggtatca gcaacattgc tcaaatccag tttcgctgcc acttcccgca
    27061 gttgcgtttt gtcggcagag agtgggtcaa tttccagata acgcaagaaa gcttgttgat
    27121 aagaaaggct ttctgctgca gggcagtcca gcacctgttg taagagatcg tccacctcgt
    27181 tcatcaaccg gtacatatca tagtgcggtc gataccactc cagcatagtg aactcagggt
    27241 tgtgataacg ccccatctct tcattacgga aactgcggca cagctggaat accggcccac
    27301 aaccggcaac cagcaggcgt ttcatatggt attccgggct ggtcattaac cagagattca
    27361 tcccctgcga atgcccgggg ccaacgaaac gtgtctcaaa cgggaccaaa tgaatatcgg
    27421 ttaccgtcgc ctggctcata caaggcgtct ccacctccag cactccacga tcggcaaaga
    27481 aacgacggat ctccgccata atcgccgcgc gttttaataa gttaggaatg gatgcgctcg
    27541 gctgccagga tgccgtttcg ctcatagtta aatctccagt ttttgacaag ggcacgaagt
    27601 ctactcgcaa cgcgacggcg agacaaattt tacgcaggaa tcaaacaacg gtgggcagtg
    27661 actaaaaaaa gcacgatctg atggtttagt aattaaatta atcatcttca gtgataattt
    27721 agccctcttg cgcactaaaa aaatcgatct cgtcaaattt cagacttatc catcagacta
    27781 tactgttgta cctataaagg agcagtggaa tagcgttcgc agaccgtaac tttcaggcac
    27841 ttaccctgaa gtacggggct gtgggataaa aacaatctgg aggaatgtcg tgcaaacctt
    27901 tcaagccgat cttgccattg taggcgccgg tggcgcggga ttacgtgctg caattgctgc
    27961 cgcgcaggca aatccaaatg caaaaatcgc actaatctca aaagtatacc cgatgcgtag
    28021 ccataccgtt gctgcagaag ggggctccgc cgctgtcgcg caggatcatg acagcttcga
    28081 atatcacttt cacgatacag tagcgggtgg cgactggttg tgtgagcagg atgtcgtgga
    28141 ttatttcgtc caccactgcc caaccgaaat gacccaactg gaactgtggg ggtgcccatg
    28201 gagccgtcgc ccggatggta gcgtcaacgt acgtcgcttc ggcggcatga aaatcgagcg
    28261 tacctggttc gccgccgata agaccggctt ccatatgctg cacacgctgt tccagacctc
    28321 tctgcaattc ccgcagatcc agcgttttga cgaacatttc gtgctggata ttctggttga
    28381 tgatggtcat gttcgcggcc tggtagcaat gaacatgatg gaaggcacgc tggtgcagat
    28441 ccgtgctaac gcggtcgtta tggctaccgg cggtgcgggt cgcgtttatc gttacaacac
    28501 caacggcggc atcgttaccg gtgacggtat gggtatggcg ctaagccacg gcgttccgct
    28561 gcgtgacatg gaattcgttc agtatcaccc aaccggtctg ccaggttccg gtatcctgat
    28621 gaccgaaggc tgccgtggtg aaggcggtat tctggtcaac aaaaatggct accgttatct
    28681 gcaagattac ggcatgggcc cggaaactcc gctgggcgag ccgaaaaaca aatatatgga
    28741 actgggtcca cgcgacaaag tttctcaggc cttctggcac gaatggcgta aaggcaacac
    28801 catctccacg ccgcgtggtg atgtggttta tctcgacctg cgtcacctcg gcgagaaaaa
    28861 actgcatgaa cgtctgccgt tcatctgcga actggcgaaa gcgtacgttg gcgtcgatcc
    28921 ggttaaagaa ccgattccgg tacgtccgac cgcacactac accatgggcg gtatcgaaac
    28981 cgatcagaac tgtgaaaccc gcattaaagg tctgttcgcc gtgggtgaat gttcctctgt
    29041 tggtctgcac ggtgcaaacc gtctgggctc caactccctg gcggaactgg tggtcttcgg
    29101 ccgtctggcc ggtgaacaag cgacagagcg tgcagcaact gccggtaatg gcaacgaagc
    29161 ggcaattgaa gcgcaggcag ctggcgttga acaacgtctg aaagatctgg ttaaccagga
    29221 tggcggcgaa aactgggcga agatccgcga cgaaatgggc ctggcaatgg aagaaggttg
    29281 cggtatctac cgtacgccgg aactgatgca gaaaaccatc gacaagctgg cagagctgca
    29341 ggaacgcttc aagcgcgtgc gcatcaccga cacttccagc gtgttcaaca ccgacctgct
    29401 ctacaccatt gaactgggcc acggtctgaa cgttgctgaa tgtatggcgc actccgcaat
    29461 ggcacgtaaa gagtcccgcg gcgcacacca gcgtctggac gaaggttgca ccgagcgtga
    29521 cgacgtcaac ttcctcaaac acaccctcgc cttccgcgat gctgatggca cgactcgcct
    29581 ggagtacagc gacgtgaaga ttactacgct gccgccagct aaacgcgttt acggtggcga
    29641 agcggatgca gccgataagg cggaagcagc caataagaag gagaaggcga atggctgaga
    29701 tgaaaaacct gaaaattgag gtggtgcgct ataacccgga agtcgatacc gcaccgcata
    29761 gcgcattcta tgaagtgcct tatgacgcaa ctacctcatt actggatgcg ctgggctaca
    29821 tcaaagacaa cctggcaccg gacctgagct accgctggtc ctgccgtatg gcgatttgtg
    29881 gttcctgcgg catgatggtt aacaacgtgc caaaactggc atgtaaaacc ttcctgcgtg
    29941 attacaccga cggtatgaag gttgaagcgt tagctaactt cccgattgaa cgcgatctgg
    30001 tggtcgatat gacccacttc atcgaaagtc tggaagcgat caaaccgtac atcatcggca
    30061 actcccgcac cgcggatcag ggtactaaca tccagacccc ggcgcagatg gcgaagtatc
    30121 accagttctc cggttgcatc aactgtggtc tgtgctacgc cgcgtgcccg cagtttggcc
    30181 tgaacccaga gttcatcggt ccggctgcca ttacgctggc gcatcgttat aacgaagata
    30241 gccgcgacca cggtaagaag gagcgtatgg cgcagttgaa cagccagaac ggcgtatgga
    30301 gctgtacttt cgtgggctat tgctccgaag tctgcccgaa acacgtcgat ccggctgcgg
    30361 ccattcagca gggcaaagta gaaagttcga aagactttct tatcgcgacc ctgaaaccac
    30421 gctaaggagt gcaacatgac gactaaacgt aaaccgtatg tacggccaat gacgtccacc
    30481 tggtggaaaa aattgccgtt ttatcgcttt tacatgctgc gcgaaggcac ggcggttccg
    30541 gctgtgtggt tcagcattga actgattttc gggctgtttg ccctgaaaaa tggcccggaa
    30601 gcctgggcgg gattcgtcga ctttttacaa aacccggtta ttgtgatcat taacctgatc
    30661 actctggcgg cagctctgct gcacaccaaa acctggtttg aactggcacc gaaagcggcc
    30721 aatatcattg taaaagacga aaaaatgggt ccagagccaa ttatcaaaag tctctgggcg
    30781 gtaactgtgg ttgccaccat cgtaatcctg tttgttgccc tgtactggta aggagcctga
    30841 gatgatcaat ccaaatccaa agcgttctga cgaaccggta ttctggggcc tcttcggggc
    30901 cggtggtatg tggagcgcaa ttattgcgcc ggtgatgatc ctgctggtgg gtattctgct
    30961 gccgctgggg ctgtttccgg gtgatgcact gagctacgag cgcgttctgg cgttcgcgca
    31021 gagcttcatt ggtcgcgtat tcctgttcct gatgatcgtt ctgccgctgt ggtgtggttt
    31081 acaccgtatg caccacgcga tgcacgatct gaaaatccac gtacctgcgg gcaaatgggt
    31141 tttctacggt ctggctgcta tcctgacagt tgtcacgctg attggtgtcg ttacaatcta
    31201 acgcatcgcc aatgtaaatc cggcccgcct atggcgggcc gttttgtatg gaaaccagac
    31261 cctatgttca aaacgacgct ctgcgcctta ttaattaccg cctcttgctc cacatttgct
    31321 gccccccaaa aaatcaacga tattgtgcat cgcacaatta ccccgcttat agagcaacaa
    31381 aagatccccg gtatggcggt ggcggtaatt tatcagggta aaccttatta ctttacctgg
    31441 ggctatgcgg acatcgccaa aaagcagccc gtcacacagc aaacgttgtt tgagttaggt
    31501 tcggtcagca aaacatttac gggcgtgctt ggtggcgacg ctattgctcg aggggaaatc
    31561 aagttaagcg atcccgcaac aaaatactgg cctgaactta ccgctaaaca gtggaacggg
    31621 atcacactat tacatctcgc aacctacacc gctggcggcc tgccattgca ggtgccggat
    31681 gaggtgaaat cctcaagcga cttgctgcgc ttctatcaaa actggcagcc tgcatgggct
    31741 ccaggaacac aacgtctgta tgccaactcc agtatcggtt tgttcggcgc actggctgtg
    31801 aagccgtctg gtttgagttt tgagcaggcg atgcaaacac gtgtcttcca gccactcaaa
    31861 ctcaaccata cgtggattaa tgtaccgccc gcagaagaaa agaattacgc ctggggatat
    31921 cgcgaaggca aggcagttca tgtttcgcca ggggcgttag atgctgaaac ttatggtgtg
    31981 aagtcgacca ttgaagatat ggcctgctgg gtacgaagca atatgaatcc ccgtgatatc
    32041 aacgacaaaa cacttcagca agggatacaa ctggcacaat ctcgctactg gcaaaccggc
    32101 gatatgtatc agggcctggg ctgggaaatg ctggactggc cggtaaatcc tgacagcatc
    32161 attaacggca gtggcaataa aattgcactg gcagcacgcc ccgtaaaagc gattacgccc
    32221 ccaactcctg cagtacgcgc atcatgggta cataaaacag gggcaaccgg cggatttggt
    32281 agctatgtcg cgtttattcc agaaaaagag ctgggtatag tgatgctggc taacaaaaac
    32341 tatcccaatc cagcgagagt caccgccgcc tggcagattc tcaacgctct acagtaaaat
    32401 ttcatcgggt ccgaattttc ggaccttttc tgcgcttttc cttgctgtca tctacactta
    32461 gaaaaaaacc agtaaggaaa catttatgcg cctgctccct ctcgttgccg cagcgacagc
    32521 tgcatttctg gtcgttgcct gcagttctcc tacgccgccg cgtggcgtga cagtagtgaa
    32581 taatttcgac gctaaacgct atctcggtac ctggtatgag attgcccgtt ttgatcaccg
    32641 ttttgaacgt ggactggaaa aagtcaccgc aacatacagt ctgcgtgatg acggcggcct
    32701 gaatgtcatc aataaaggct ataaccctga cagagaaatg tggcagcaga gtgaagggaa
    32761 agcgtacttt accggcgcac caacacgcgc tgcgctgaaa gtgtcattct ttggtccttt
    32821 ctatggcggt tataacgtca ttgcacttga tcgggaatac cgccatgcgc tggtttgcgg
    32881 cccggaccgc gactacctgt ggatactctc ccgcacgcca accatttctg acgaagtgaa
    32941 acaggagatg ctggcagtcg cgacccggga agggtttgat gtcagtaagt ttatttgggt
    33001 acagcagccc ggtagttagt gagtgctgag tttcagacca ataatcccca atacgattaa
    33061 cgccagactc gccaggcgca tcgggttagc ggactcaccg agcagtacaa tgccggtgat
    33121 agccgcgccg actgcgccaa tacccgtcca cacggcataa gccgtcccta ctggtaacga
    33181 tttcatcgcc caggcaagta gcgccatact gacaatcatc gccgtcacgg taataacact
    33241 cggcgtcaaa cgactaaagc cgtgggtata tttcaggcca acggcccata ccacttccag
    33301 cagaccagca ataactaaga taatccagga catatcaggc tccggagaat tggggccgtc
    33361 cccggtgaaa agacgcgttt gcaggtcgtc ctgcaaagct gatggttgaa cggatatttt
    33421 gccaggtggc aggggaattt caactattac cttagtgact atcgcagaaa aaacaaacgg
    33481 cacgacacag ctgtcgtacc gttattgctt attgctattg ctgcgctttc gttgctgcgc
    33541 cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg
    33601 cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca
    33661 taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca
    33721 acatcgccaa aaatgacgga aatctgatga tttttcggaa gagaagaaaa attagctggc
    33781 agcgcgagag atggagttgc cgagatgttt gatgtcttcg ccgaaaccgc gagcggtgtt
    33841 acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc
    33901 tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg
    33961 agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag
    34021 cggaacctta accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt
    34081 atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt
    34141 aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca
    34201 tttagcgtta tcgccaattg cttttgcatc agcagacagc tgctcgaaag tttcgttgtt
    34261 catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc
    34321 agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag
    34381 acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc
    34441 ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct
    34501 atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg
    34561 cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg
    34621 ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt
    34681 tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc
    34741 gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc tcgcaagatg
    34801 agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac agcgtagtgc
    34861 ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg
    34921 taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc
    34981 gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac gagatgattt
    35041 tctctggcgg cgatccgctg atggcgaaag atcacgagct ggactggttg ctcacacaac
    35101 tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg attgtgatcc
    35161 cggcacgcat cacagatgcg ctggttgaac gcttttcaca ttctacgctg caaatcttgc
    35221 tggtgaatca catcaaccat gccaatgaga tagatgaaac attccgtcag gcgatggcta
    35281 agttgcgccg ggtaggcgtt actttgctga accagagcgt tctgttacgt gatgtgaacg
    35341 ataacgcaca aacgctggca aacctgagta atgcgttgtt cgatgccggc gtgatgccgt
    35401 attacctgca tgtgctcgat aaagtacagg gcgcggcgca ttttatggtg agtgatgacg
    35461 aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg gtgccgaaac
    35521 tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag ctacgccagc
    35581 agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta cgcttatcag
    35641 gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt
    35701 ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat taaactgaga
    35761 aatttaatct ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt
    35821 tttatatagc taacaccaat aattaacctt tcttttctgt ttatata
//
LOCUS       DAJOSY010000035        35367 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJOSY010000035 DAJOSY010000000
VERSION     DAJOSY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.035"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..964
                     /gene="ldhA"
                     /locus_tag="OHN27_001858"
     CDS             <1..964
                     /gene="ldhA"
                     /locus_tag="OHN27_001858"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCQ2286306.1"
                     /translation="QYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVN
                     DDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEAVAEHAIG
                     MMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMR
                     LLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFDQMKNGV
                     MIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLS
                     ACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1075..1497
                     /gene="hslJ"
                     /locus_tag="OHN27_001859"
     CDS             1075..1497
                     /gene="hslJ"
                     /locus_tag="OHN27_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCQ2286307.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLVTAKQTLTYKLADLMN"
     gene            complement(1494..1760)
                     /locus_tag="OHN27_001860"
     CDS             complement(1494..1760)
                     /locus_tag="OHN27_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCQ2286308.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2034..5558
                     /gene="nifJ"
                     /locus_tag="OHN27_001861"
     CDS             2034..5558
                     /gene="nifJ"
                     /locus_tag="OHN27_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCQ2286309.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISQIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQVEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCLLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPKVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            5925..7058
                     /gene="ompN"
                     /locus_tag="OHN27_001862"
     CDS             5925..7058
                     /gene="ompN"
                     /locus_tag="OHN27_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCQ2286310.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            7199..7633
                     /gene="uspF"
                     /locus_tag="OHN27_001863"
     CDS             7199..7633
                     /gene="uspF"
                     /locus_tag="OHN27_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCQ2286311.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEEEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            8220..9134
                     /gene="sitA"
                     /locus_tag="OHN27_001864"
     CDS             8220..9134
                     /gene="sitA"
                     /locus_tag="OHN27_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCQ2286312.1"
                     /translation="MYSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDMVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            9134..9961
                     /gene="sitB"
                     /locus_tag="OHN27_001865"
     CDS             9134..9961
                     /gene="sitB"
                     /locus_tag="OHN27_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCQ2286313.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKNT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            9958..10815
                     /gene="sitC"
                     /locus_tag="OHN27_001866"
     CDS             9958..10815
                     /gene="sitC"
                     /locus_tag="OHN27_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCQ2286314.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            10812..11669
                     /gene="sitD"
                     /locus_tag="OHN27_001867"
     CDS             10812..11669
                     /gene="sitD"
                     /locus_tag="OHN27_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCQ2286315.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFIAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGNMLGVTIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVISFNARTKGNA
                     HRQDLSSPN"
     gene            complement(11883..12011)
                     /locus_tag="OHN27_001868"
     CDS             complement(11883..12011)
                     /locus_tag="OHN27_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCQ2286316.1"
                     /translation="MEIATEEEKALLAAWKKYRVLLNRVDTSTAPDIEWPAVPVME"
     gene            complement(12066..15824)
                     /locus_tag="OHN27_001869"
     CDS             complement(12066..15824)
                     /locus_tag="OHN27_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCQ2286317.1"
                     /translation="MAVKISGVLKDGAGKPVQNCTIQLKAKRNSTTVVANTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSAREAATRATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEALKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARDASASKVAAKSSETSAASSAGSAASSATAAGNSAKAAKT
                     SETNADNSAQAAADSQTASANSATSAKKSETNAKNSEAAAKVSETNAKASENKAKEYL
                     DKVGGLVSPMTQYDWPVVTGSESFYIKIAKLSDPGSNNCHVTLMVTNAGNYGSPYGNI
                     DFIEISARGLPSLLSADNVSRHLSIRRLGSTGLTDNNQMRYGLVKGDGFIEVWAFQGA
                     FINDAKVAVLAQTTLNTELYIPDGFVKQTAAPSGYIEGNVVRIYDQVNKPTKADLGLS
                     NAMLTGAFGLGGSGISTNGKMSDVEILKALRDKGGHFWRGDKPTGSTATIYSHGSGIF
                     SRCGDTWSAINIDYSTAKIKIYAGNDARLNNGTFSVNELYGTKNKPSKSDVGLGNVTN
                     DAQVKKSGDVMSGDLDILKETPSIRLKSAKGTAHLWFMNNDGERGVVWAPVNTDTLGE
                     VRIRAKNAKGESSGDFVVRHDGRVDARDLKVTYKISGATAELANTSTDVANTTLKISG
                     SQHTPLVLTRSGEIKNLSIGFKVDDVEQKYLGVYGDGNLYFGNSSDHSKNSRVITQAI
                     LDSGVTVGGKTTFSDLATFNAGISGAIEPESISEVTVDLNDLTIKGTDKGSVKYYQCR
                     TAGGGANITNKPDGVGGNFLLRVESIRKIRDSDYANMQTLIGSDTKRIYVRFVVNGNW
                     TAWSQVVVSGWNQDVTVRSLTSTTPSKLGGGRVDVLGSTSDYNSMNCAVRGVDNTGTN
                     SAWSVGTSESTGKMLFLKNHRSSAQVLLNGDDGAVQLKTGAVNGATAQALTINRNEVN
                     STVDLTLTKQSGTGNRFVLQNSGNAELPFSVRVWGSSTRQNVFEVGTSAAYLFYAQKT
                     TDGQNLTVNGSVNCTTLNQSSDRRLKENIEIIDNATDAIRKINGYTYTLKENGAHCAG
                     VIAQEVEEAIPEAVGSFIHYGEELQGPTVDGNELREETRYLNVDYAAVTGLLVQVARE
                     TDDRVTALEEENTTLRENLATADTRISTLENQVSELVALVRQLTGSEH"
     gene            complement(15889..16488)
                     /locus_tag="OHN27_001870"
     CDS             complement(15889..16488)
                     /locus_tag="OHN27_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCQ2286318.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMVG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSSDWRTDGFIVGVGYKF"
     gene            complement(16556..20035)
                     /locus_tag="OHN27_001871"
     CDS             complement(16556..20035)
                     /locus_tag="OHN27_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCQ2286319.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGHYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPVAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVG
                     KAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHERENNDIYSSCRLI
                     VRKNGAEIYNRTALDNTLIYSGVIDMPAGRGHMTLEFSVSAWLVNNWYPTASISDLLV
                     VVMKKATAGISIS"
     gene            complement(20095..20727)
                     /locus_tag="OHN27_001872"
     CDS             complement(20095..20727)
                     /locus_tag="OHN27_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCQ2286320.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPTAASTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(20664..21407)
                     /locus_tag="OHN27_001873"
     CDS             complement(20664..21407)
                     /locus_tag="OHN27_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2286321.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFYREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASIFV"
     gene            complement(21413..22111)
                     /locus_tag="OHN27_001874"
     CDS             complement(21413..22111)
                     /locus_tag="OHN27_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCQ2286322.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYACFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(22111..22440)
                     /locus_tag="OHN27_001875"
     CDS             complement(22111..22440)
                     /locus_tag="OHN27_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2286323.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVSRWEAAALESFLAEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(22437..24992)
                     /locus_tag="OHN27_001876"
     CDS             complement(22437..24992)
                     /locus_tag="OHN27_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCQ2286324.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQFA
                     VLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFAQQ
                     QRAKRAAIDAKKRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQLR
                     GSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSV
                     LSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTG
                     GKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGIN
                     FNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            complement(24985..25419)
                     /locus_tag="OHN27_001877"
     CDS             complement(24985..25419)
                     /locus_tag="OHN27_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCQ2286325.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(25401..25823)
                     /locus_tag="OHN27_001878"
     CDS             complement(25401..25823)
                     /locus_tag="OHN27_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCQ2286326.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            complement(25839..26579)
                     /locus_tag="OHN27_001879"
     CDS             complement(25839..26579)
                     /locus_tag="OHN27_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2286327.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            complement(26587..26982)
                     /locus_tag="OHN27_001880"
     CDS             complement(26587..26982)
                     /locus_tag="OHN27_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2286328.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(26979..27557)
                     /locus_tag="OHN27_001881"
     CDS             complement(26979..27557)
                     /locus_tag="OHN27_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2286329.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVAQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(27569..27922)
                     /locus_tag="OHN27_001882"
     CDS             complement(27569..27922)
                     /locus_tag="OHN27_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCQ2286330.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(27934..28329)
                     /locus_tag="OHN27_001883"
     CDS             complement(27934..28329)
                     /locus_tag="OHN27_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCQ2286331.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(28371..29396)
                     /locus_tag="OHN27_001884"
     CDS             complement(28371..29396)
                     /locus_tag="OHN27_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCQ2286332.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            complement(29452..29784)
                     /locus_tag="OHN27_001885"
     CDS             complement(29452..29784)
                     /locus_tag="OHN27_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCQ2286333.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSSTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(29794..31113)
                     /locus_tag="OHN27_001886"
     CDS             complement(29794..31113)
                     /locus_tag="OHN27_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCQ2286334.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(31094..32695)
                     /locus_tag="OHN27_001887"
     CDS             complement(31094..32695)
                     /locus_tag="OHN27_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCQ2286335.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            complement(32692..32898)
                     /locus_tag="OHN27_001888"
     CDS             complement(32692..32898)
                     /locus_tag="OHN27_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCQ2286336.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            complement(32895..34820)
                     /locus_tag="OHN27_001889"
     CDS             complement(32895..34820)
                     /locus_tag="OHN27_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCQ2286337.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWVKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLQRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            complement(34795..35340)
                     /locus_tag="OHN27_001890"
     CDS             complement(34795..35340)
                     /locus_tag="OHN27_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCQ2286338.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
BASE COUNT         7817 a        10145 c         8821 g         8584 t
ORIGIN      
        1 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
       61 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
      121 tattttcgta aacgatgacg gcagccgccc ggtgctggaa gagctgaaaa agcacggcgt
      181 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgacg cggcaaaaga
      241 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
      301 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
      361 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
      421 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
      481 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
      541 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
      601 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
      661 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
      721 tcagaaaatt ggttcgttgg gtatggacgt atatgagaac gaacgcgatc tgttctttga
      781 agataaatcc aacgacgtaa ttcaggatga cgtattccgt cgcctgtctg cctgccacaa
      841 cgtgctgttt accgggcatc aggcattcct gacagcagaa gctctgacca gtatttctca
      901 gactacgctg caaaacttaa gcaatctgga aaaaggcgaa acctgcccga acgaactggt
      961 ttaatcttgc cgctcccctg cattccaggg gagcttattc agataatccc caaagacctt
     1021 tcatcctcta ttcttaaaat agccctgagt cagaaactgt aattgagaac cacaatgaag
     1081 aaagtagccg cgcttgttgc gctaagcctg ctgatggcgg gatgtgtaag tagtgacaaa
     1141 attgctgtaa cgccagaaca gttacagcat catcgctttg tgctggaaag cgtaaacggt
     1201 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     1261 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     1321 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     1381 aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta
     1441 acgctggtga ccgcgaaaca gacattaact tataagctgg cggatttaat gaattaatag
     1501 ctgccacagc tcccggcggc aagtgactgt tcactacagc gtttgccgtt gggtaatgca
     1561 cacatcccaa tcgccgtacc atccagttga cgggcaacag aaagcgaacc gccgatcatt
     1621 gcacaatttg cttctccact actggacatc gacgctttta aacctggcgc tacgtgcgcc
     1681 gcagtcgcct gctgaacagg ctcactacta cacgccgaca acaataaagc ggcacaccct
     1741 acccaaaacg ctgctcgcat cttttcttcc tctgatcttc aagccaaacg acaccgccat
     1801 aaataatagg cagcacagag ggcggcgtcg agagctgtcc tgcgcgttgc cccgcatttt
     1861 tactttttta tggctatttt tttgccctct gtttgatcaa aacattcatt acgctgatgt
     1921 gggggacaca aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa agattactac
     1981 tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt catatgatta
     2041 ctattgacgg taatggcgcg gttgcttcgg tcgcatttcg caccagtgaa gttatcgcca
     2101 tctaccctat tacccccagt tccacgatgg cagaacaggc tgatgcctgg gccggaaacg
     2161 gcttaaagaa cgtttgggga gacacaccac gcgtggttga aatgcagtcg gaagcgggtg
     2221 ctatcgctac cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg tttacgtcat
     2281 cgcagggttt gctgctgatg atcccgacgc tgtacaaact ggcaggcgaa ctaacaccgt
     2341 ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc actctctatt tttggcgatc
     2401 attccgacgt tatggcggtg cgccagacgg gttgcgcgat gttgtgtgca gcaaacgtcc
     2461 aggaagcgca agactttgct ctcatttcgc aaatcgcgac gctgaaaagc cgcgtgccat
     2521 ttattcattt ctttgatggt ttccgcacgt cccacgaaat caataaaatt gtcccgctgg
     2581 ccgatgacac gattcttgat ctcatgccgc aggtcgaaat tgatgctcat cgcgcccggg
     2641 cactcaaccc ggaacatccg gtgatccgcg gtacgtccgc caatcctgac acttatttcc
     2701 agtctcgcga agccaccaac ccatggtaca acgcggtcta tgaccatgtt gaacaggcga
     2761 tgaatgattt ctctgccgcg acaggtcgtc agtatcagcc gtttgaatat tacgggcatc
     2821 cgcaagcgga acgggtgatt atcctgatgg gctctgccat tggcacctgt gaagaagtgg
     2881 ttgatgaatt gctaacccgt ggcgaaaaag ttggcgtgct gaaagttcgc ctgtaccgcc
     2941 ccttctccgc taaacattta ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac
     3001 tggacagaac caaagaaccc ggtgcccagg cagaaccgct ctatctggat gtaatgaccg
     3061 cactggcaga agcctttaat aatggcgagc gcgaaactct gccccgtgtc attggtgggc
     3121 gctatggtct ttcatccaaa gaatttggcc cagactgttt actggcggta tttgccgagc
     3181 tcaacgcggc taaaccgaaa gcgcgcttta cggttggtat ttacgatgat gtgaccaatc
     3241 tgtcactgcc gttgccggaa aacaccctgc caaactcggc gaaactggaa gccttgtttt
     3301 atggccttgg tagtgatggc agcgtttccg cgaccaaaaa caatatcaag attatcggta
     3361 attccacgcc gtggtacgca cagggctatt ttgtttacga ctccaaaaag gcgggcggcc
     3421 tgacggtttc tcaccttcga gtgagcgaac agccgattcg ttccgcttat ctcatttccc
     3481 aggctgattt tgttggctgc caccagttgc agtttatcga taaatatcag atggctgagc
     3541 gtttaaaacc tggcggcatt ttcctgctca acacaccgta cagcgcagat gaagtgtggt
     3601 cacgcttgcc gcaagaagtt caggccgtgt taaaccagaa aaaagcgcgc ttctatgtga
     3661 ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc
     3721 agatggcttt tttccatctg acgcaaattc tgcctggcga tagcgccctc gcagaattgc
     3781 agggtgcgat tgccaaaagt tacagtagca aaggccagga tctggtggaa cgcaactggc
     3841 aggctctggc gctggcgcgt gaatccgtag aagaagttcc gttgcagccg gttaatccgc
     3901 acagcgccaa tcgaccgcca gtggtttccg atgccgcccc tgatttcgtg aaaaccgtaa
     3961 ccgctgcgat gctcgccggg cttggtgatg ccctccccgt ttcggcgctg ccgccagacg
     4021 gcacctggcc gatgggcacc actcgctggg aaaaacgcaa tatcgccgaa gagatcccca
     4081 tctggaaaga ggaactctgt acccaatgta accactgcgt tgccgcttgc ccacactcag
     4141 ctattcgcgc aaaagtggtg ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt
     4201 cgctggatgt gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag gtggcaccgg
     4261 aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc
     4321 cagagattaa agccatcaat atgatgtctc gcctggaaca tgtcgaagaa gagaaaatca
     4381 attacgattt cttcctcaac ctgccagaaa tcgaccgtag caaactggaa cgtattgata
     4441 ttcgtacatc gcagctgatt acaccgctgt ttgaatattc aggtgcttgc tccggttgtg
     4501 gcgagacgcc gtatattaaa ttactgactc agctctatgg cgaccggatg ttgatcgcta
     4561 acgccactgg ctgttcttca atttatggcg gtaacctgcc ctctacaccg tataccaccg
     4621 atgccaacgg tcgtgggccg gcatgggcga actctctatt tgaagataat gccgaatttg
     4681 gccttggttt ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc
     4741 aatttgccga taaaatcccg gcggaattac tgacggcgtt gaaatcagac gccacgccag
     4801 aggttcgtcg tgaacaggtt gcagctttac gccagcaact caacgatgtt gccgaagcac
     4861 atgaactgct acgtgatgca gatgcactgg tggaaaaatc aatctggctg attggtggtg
     4921 atggctgggc ttacgatatc ggctttggcg gtctggatca tgtattgagt ttgacggaaa
     4981 acgtcaacat tctggtgctg gatacgcaat gctattccaa caccggtggt caggcgtcga
     5041 aagcgacacc gctgggtgca gtaactaaat ttggcgagca cggcaaacgt aaagcgcgta
     5101 aagatcttgg cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag atttctctcg
     5161 gcgcgcagct gaaccagacg gtgaaagcga ttcaggaagc ggaagcgtat ccggggccat
     5221 cgctgatcat tgcttatagc ccgtgtgaag agcatggtta cgatctggca ctcagccacg
     5281 accagatgcg ccaactcaca gctaccggct tctggccgct atatcgcttt gatccgcgtc
     5341 gtgccgatga aggcaaactg ccgctggcct tggattcacg cccgccgtca gaagcactgg
     5401 aagaaacgtt acttcacgag caacgtttcc gtcggctgaa ttcgcagcag ccaaaagtgg
     5461 cagaacagtt atggaaagat gctgcagctg atttgcaaaa acgctatgac ttcctggcac
     5521 aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat gctctggata aggattatcc
     5581 aattctaaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc
     5641 agtggcaatg caatggccca acagaaaata aaatctgtga caataaaggc atataacccg
     5701 cgcagaataa cgtatagcaa attaaaagta taatttttat tttgtatgta ttatcttcga
     5761 tacattcacc agacttatta tcatttcaga ggaattattt gattaaggtt ttacttaagg
     5821 cgtaacaaat gatatttatc ggctaactga acttctcctt taggatgttt tcactccccg
     5881 aaatggggat aaaaggcaaa taaaataact aaaggattta ttcaatgaaa agcaaagtac
     5941 tggcactttt aattcctgcc ctgctcgccg caggtgctgc acatgcagcc gaagtttata
     6001 ataaagacgg caacaaatta gatctgtatg gcaaagttga tggcctgcat tatttttctg
     6061 ataattcagc gaaagatggc gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc
     6121 aaattaacga tcaactcact ggctacggtc aatgggaata caatattcag gcaaacaaca
     6181 ctgaatcttc aaaaaaccag tcatggaccc gtctggcatt tgccgggctg aaatttgcag
     6241 attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc gaaggctgga
     6301 ccgatatgct gcctgaattt ggcggtgact cttataccaa tgcagacaac tttatgactg
     6361 gtcgagccaa tggcgtcgcg acttatcgta atactgattt cttcggtctg gtaaatggtc
     6421 tgaacttcgc ggtgcagtat caaggtaaca acgaaggagc cagtaatggt caggaaggca
     6481 ccaacaacgg acgtgatgtt cgccatgaaa acggtgacgg ctggggtctt tccacaacat
     6541 atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac cgcaccaatg
     6601 accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact gctgggctaa
     6661 aatacgatgc taacaatatt tacctggcaa ccatgtattc agaaacgcgt aatatgaccc
     6721 cgtttggcga cagcgattat gctgtggcaa acaaaaccca gaattttgaa gtcactgcac
     6781 agtaccagtt tgattttggt ctgcgtccgg cagtctcttt cctgatgtct aaaggccgtg
     6841 acctgcacgc tgcgggtggt gcagacaacc cggcaggtgt tgatgataaa gatctggtta
     6901 aatacgccga tgttggcgcg acttactatt tcaataaaaa catgtccacc tacgttgact
     6961 ataaaatcaa cctgttggat gaagatgaca gcttctacgc tgccaatggc atctctaccg
     7021 atgatattgt cgctttaggt ctggtttatc agttctaaat cctcctgccc gctgttatgg
     7081 cgggcttttt ctgcttattc ttcctccttt gatctaaatt aaaaatgtga actccgtcat
     7141 tacacaaaaa gtgtcatctg gcgttacact ttatgcggat actaaaacag gaggttttat
     7201 gaacagaacg attcttgtcc ctatcgatat ttccgattca gaattaactc aacgcgtgat
     7261 tagccacgtt gaggaagagg caaagattga tgatgcagag gttcatttcc tgacggtaat
     7321 accttcactg ccctactatg cctctctggg tttagcgtat tccgcagaat taccggcaat
     7381 ggatgacctg aaagcggaag ccaaatcgca actggaagag atcattaaaa aatttaaact
     7441 gccaaccgac agagtgcatg tccatgttga ggaaggctcg cccaaagacc gcattctgga
     7501 attggcgaag aagatccccg ctcatatgat catcattgct tcccatcgac cggatatcac
     7561 cacttatctg ctcggttcca acgccgcagc tgtagtgcgt cacgcagagt gctccgtgct
     7621 ggttgtgcgc tgacactaac gcccgcacat tgctgcgggc tttttgattc atttcgcaaa
     7681 tgtgctgaca ttttcccctc taatccgtac catacacgcc acagtttttt atatcagatt
     7741 tcttatgctg ggcgttccgg catgacgcat actcttctga tgccatataa cgaattgagt
     7801 cgcttttaaa tgtcgcaaaa tcaagaaatt agtaagaaag aacaatacaa cctgaacaag
     7861 taagggcaaa aatcacaact ctctgactca taagtatttt acttattttt caatgtgtta
     7921 aatatcatca gcgacaacaa taagctacga taacccactc tttttttgcc ccttttttgc
     7981 cccttttgca gcgctttgcc ccatttttgc catcaacaat tttcctcatc aagtcatttc
     8041 aaagcctaat aggtgcccta cttttttcct cagtaccacg tcgccagcag gaaacaacct
     8101 aatccctgtt tcggacctaa aattgctaat gataatcatt ttcattttgt atggcttatg
     8161 ctataaaaca tagccgttgc tatgtttgtt ctaattttgc tcactatagg tactaaatta
     8221 tgtactcaat aaaaaaagta accatgctct tggggtgcct cgccctcacc tgctcgatcg
     8281 catttcaggc gagtgcaact gaaaaattca aagtcattac aacatttacc atcatcgcag
     8341 atatggccaa aaacgtggct ggagatgctg cagaagtctc atccataacc aagcccggtg
     8401 cagaaattca tgagtatcag cctacccctg gtgatattaa acgtgcgcag ggggcacaac
     8461 tgattctcgc caatggtatg aatctggaat tgtggttcca acgcttttac cagcatctta
     8521 atggggttcc agaagtaatt gtctcttcgg gtgtgacgcc agtagggatc actgaaggcc
     8581 cctatgaggg caaacctaac ccccatgcct ggatgtcgcc agataatgct ctgatttacg
     8641 tcgataatat tcgtgatgcg ttgataaaat acgatccggc aaatgcacaa acctaccaac
     8701 gtaatgccga tacttataaa gccaagatta cccaaaccct tgcccccctg cgtaagcaga
     8761 ttgctgaact ccctgagaat cagcgatgga tggtcaccag tgaaggggct ttttcttatc
     8821 tcgcacgcga tttggggcta aaagagcttt atctgtggcc gattaatgcc gatcaacaag
     8881 gcacgccgca gcaggtacgt aaggttgttg atatggtgaa gaaaaatcat atcccggcag
     8941 tctttagcga gagtacgatt tccgataaac cagcgcgtca ggttgcgcgt gaaaccggcg
     9001 cgcactacgg cggtgtactc tatgtcgatt ccctgagcac agaaaacggc ccggtaccta
     9061 cgtatatcga ccttctgaaa gttactacca gtacgctggt acagggaatt aaagccggga
     9121 aaagggagaa ataatgatgc aatctgcggg cattgtcgtt aatgatgtta ctgtcacctg
     9181 gcgtaacgga cacacagcac tgcgcgatgc atccttcaca gtaccaagtg gatctattgc
     9241 cgctctggtt ggggtaaacg gttccggaaa atcgacgttg tttaaagcga ttatgggatt
     9301 tgtgcgtctg accagcggaa agatatctgt tctgggtatt cccacgcgac aggcgctaca
     9361 gaaaaacctg gttgcctacg tccctcagtc agaagaggtt gactggtcat ttcctgtact
     9421 agtggaagat gtggtgatga tgggccgcta tgggcacatg ggtatgttgc gtatagccaa
     9481 aaagagggat cgtcagattg tcaccgatgc gctggaacgt gtcgatatgg tagattttcg
     9541 ccatcgacaa atcggcgagc tttccggtgg acaaaagaaa cgcgtctttc tggcgagagc
     9601 gattgcacag cagggtgatg tgatcctgct ggatgaacct tttaccggcg ttgatgtgaa
     9661 aaccgaagcg aaaatcatca gcctgttgag ggagttacgc gcggaaggca agacgatgct
     9721 tgtctcaacg cacaatctcg gctcagtcac gacattttgc gattatacgg tcatggtcaa
     9781 aaacaccgtc ctggcaagcg ggccgacaga caccactttt acagccgaga acctggagct
     9841 ggcttttagc ggcgtactgc gccatgtcac acttaacggc tcggaagaaa gcattattac
     9901 tgacgatgaa cgcccttttg tggcacatcg accgtcagcg gtgcagaggg aagcaagatg
     9961 aacgtgctac tggaaccctt cagctatgaa tatatgctca atgcgatgtg ggtctcggcg
    10021 atggttggcg gtctctgcgc tttcctgtca tgctatctga tgctaaaagg ctggtcgctg
    10081 attggcgatg cgctgtcgca ctctattgtc ccgggtgttg cgggagcata tatgctgggg
    10141 cttccgtttt ctctgggagc cttcttttcc ggcggactgg cagcgggcag catgctgttt
    10201 cttaaccaac gcactcgctt aaaagaagat gccatcatcg gcctgatctt ctcctccttt
    10261 tttggcctgg ggctgtttat ggtgtcattg aacccgacat ccgtgaacat tcagaccatc
    10321 gtattgggca atattctggc aatcgatccg gcggatatcc tgcaactcac tatcattggc
    10381 attctctcga tcatcgtgct ttttttcaaa tggaaagatc ttatggtgac ctttttcgac
    10441 gagaatcacg cccgtgctat tggattgcat ccgggcagat taaaaatcct cttcttcacc
    10501 ctgctgtcgg tttcgaccgt tgcggcgctg caaacggttg gtgcctttct ggtgatctgt
    10561 ctggtggtga ctcccggtgc aacagcatgg ttgttaaccg atcgttttcc ccgcctgctc
    10621 ataattgctg taactattgg cagtgtaacc agtttcctcg gcgcgtgggt cagttacttt
    10681 ctggacggcg cgacaggtgg aatcatcgtg gtagcgcaga cactgctgtt tcttttagca
    10741 tttgtatttg cgcccaccca tgggcttttg gcaaatcgtc ggcgtgcgca taaagcgctg
    10801 gaggatcgct catgatggca ttattactgg agccactgca gtttaccttt atgagtcacg
    10861 cactgctgat ttcactggta gtttccatac cctgtgcgct gctgtcggtc tttctggtcc
    10921 tgaaaggttg ggcactgatg ggtgacgcga tgagtcatgc ggtttttcct ggcatcgtgc
    10981 ttgcctggat tctggggtta cctctggcta caggggcatt tatcgccgga gtattctgcg
    11041 cggtggcgac gggatacctg aaagacaaca gccgaatcaa gcaagataca gtgatgggga
    11101 ttgttttttc tggcatgttt gccgcaggtc tgatcttgta tattgcagtc aaaccagacg
    11161 tacatcttga ccatattctt ttcggcaaca tgctcggggt aaccatcggc gatataatcc
    11221 agacggtgat tattgccggg ctggttacac ttgttattag cgtaaaatgg cgggattttt
    11281 tgctgttcag ctttgattat caacaggcgc aggtaagcgg tttgcatacg agatggctgc
    11341 attatgggct gctgtgcatg gtttccctga ccattgtggc aacgctgaaa gcagtaggta
    11401 tcattctctc catttctctg ctcattgcgc ctggcgccat tgcggtactc ctgacacaac
    11461 gctttcatat tgcgctccta ctggcgactg gcatatcggt aatagtctca atgactggtg
    11521 tctggctgtc cttttttatc gacagcgcac cagctccgac gattgtcgtc ttattcgcgg
    11581 ttgtgttcat catgacgttt accgttatca gcttcaacgc acgcacaaag ggaaacgctc
    11641 atagacagga tttgtcatca cccaattagc acaacatccg aggacagcat cgtccgctgg
    11701 cccacaaaag tccggaaagt catggaaggt cgttgttggg aggaggtaca aattctctca
    11761 tgcaaaaaat acgtaaaatc gataatagct ggaaataatt caatacatgc actctcgaaa
    11821 gtgcatcagc cagccgcagc acgttcctgc atacaacgtg tctgcggtat atcacaaaac
    11881 gattactcca taacagggac agcaggccat tcaatatcag gtgcagttga tgtatcaaca
    11941 cggttcagca acacccgata ctttttccat gcggccagta acgctttctc ttcctccgtt
    12001 gcgatttcca gctcaacaac agtctgaacg taccgggaac agcctccttc agagcttgaa
    12061 ggatatcaat gttcgcttcc tgttaactgc cggacaagtg caaccagttc gcttacctga
    12121 ttttccagag tgctgatccg ggtgtctgct gttgccagat tttcacgtag cgttgtgttt
    12181 tcctcttcca gcgcggtaac gcgatcatct gtttcacggg cgacctgaac aagtaaaccc
    12241 gtcacggcgg cgtagtcaac attaagatag cgcgtttctt cgcgtagctc gttgccgtca
    12301 acggtcgggc cttgcaactc ttcaccgtaa tggatgaatg aaccaacggc ctccgggatg
    12361 gcctcttcaa cttcctgggc tataaccccg gcgcaatgtg ccccattctc cttgagcgtg
    12421 tacgtgtatc cattaatttt gcggattgcg tcagtcgcgt tatcgataat ctcgatattt
    12481 tctttcagtc tgcggtctga tgactgattc agtgtggtgc agttaacact tccgtttaca
    12541 gtaaggtttt gcccgtctgt tgttttttgc gcataaaaca gatacgcagc agacgttcca
    12601 acctcaaaaa cgttttgacg agtactggaa ccccacaccc tgacagaaaa cggtagttct
    12661 gcattacctg agttctgtaa aacaaaacga ttaccagtcc ctgattgctt tgtaagggtt
    12721 aaatcaacgg ttgagttaac ctcattcctg ttgatagtga gcgcctgcgc tgtagccccg
    12781 ttaacagcac ctgttttgag ttgaaccgcg ccatcatcgc catttaacag cacttgagcg
    12841 ctgcttctgt ggtttttcag gaacaacatt ttgcctgtgc tttctgatgt acctaccgac
    12901 cacgccgaat tggttccagt gttatcaaca ccacgcacag cacaattcat gctgttgtaa
    12961 tctgacgtac ttccaagcac gtcaacacgc ccgccaccta attttgatgg agtcgtcgag
    13021 gttaacgacc tgacggttac atcctgattc catccggaaa caacaacctg actccacgct
    13081 gtccagtttc cattaacaac aaagcgaacg tatatacgtt ttgtgtcgct gccaatcagc
    13141 gtttgcatgt tcgcataatc tgaatcccta atcttacgaa tcgactctac acgaagcaaa
    13201 aagttaccgc ctacgccgtc aggcttattg gtaatattcg caccacctcc ggcagttctg
    13261 cactgatagt atttaaccga tcctttatct gtgcctttaa tggtcagatc attaagatca
    13321 acggtcactt cagaaatgct ttcaggttcg attgcgccag atatgccagc gttaaatgtg
    13381 gcaaggtcag aaaatgttgt tttaccgcct accgttaccc cgctatcgag tattgcttgt
    13441 gtaattactc tgctgttttt tgagtggtca gaggaattac cgaaatacaa attaccgtca
    13501 ccgtatacgc cgagatattt ttgttcaaca tcatcaacct taaacccaat ggataagttt
    13561 ttaatttctc cactgcgagt taaaactaaa ggcgtatgct gtgatccgct gatttttaac
    13621 gtagtattag ccacgtcagt tgatgtgttt gcaagctctg cggtggctcc actgattttg
    13681 tacgttactt ttagatcgcg ggcatcaacc ctcccgtcgt ggcgaacaac aaaatcacca
    13741 cttgattcac cttttgcgtt tttagccctg atgcgaactt cgccaagcgt atcagtattt
    13801 acaggcgccc aaacaacgcc gcgctcgccg tcgttgttca tgaaccacag atgagcggtt
    13861 ccttttgctg attttagcct gatagacggc gtttctttca atatatcaag atcacctgac
    13921 ataacatcgc cggatttttt cacctgcgca tcgttagtta cgttgccgag tccaacatcc
    13981 gatttcgacg gcttgttttt ggttccgtat agctcattga cgctaaaagt cccgttgtta
    14041 agccgggcat cgttgccggc atagatctta atcttcgcgg ttgagtagtc gatattgatc
    14101 gctgaccacg tatcgccgca ccgcgagaat ataccagaac cgtggctata aatggtcgcc
    14161 gtgcttccgg tcggcttatc accgcgccag aaatgaccac ctttgtcacg cagagctttt
    14221 aagatctcta catcgctcat cttgccgttt gttgatatcc cgctaccgcc aagaccgaaa
    14281 gcgcctgtaa gcatagcatt agaaagaccc aaatctgctt tagtcggctt gtttacctgg
    14341 tcgtaaatcc ttacaacgtt gccttcaata tatccagaag gcgcggcggt ttgcttaaca
    14401 aatccgtctg gaatgtataa ttctgtgttt agtgttgtct gcgccagcac cgcaaccttt
    14461 gcgtcgttaa taaacgcacc ctggaatgcc caaacctcaa taaagccgtc acctttaacc
    14521 aggccgtaac gcatctggtt gttatcggtc agcccggttg accctaagcg gcgtatactc
    14581 agatgacgag aaacattatc cgcactaagc aaagaaggca gaccgcgcgc cgagatctcg
    14641 ataaagtcaa tgtttccgta aggggagccg tagttaccgg cattagtaac cattagcgtt
    14701 acatggcaat tgttgcttcc aggatcggaa agtttcgcga tctttatgta aaaagactca
    14761 ctaccagtaa caacgggcca atcgtattgc gtcatcgggc tgacgagtcc cccgaccttg
    14821 tcgagatatt ctttcgcctt gttctctgac gctttagcgt tggtttcgct gacctttgct
    14881 gctgcctcac tatttttcgc gttggtttct gattttttgg ctgatgtcgc ggagtttgcc
    14941 gatgcagttt gtgagtctgc tgccgcctgt gcgctgttat ccgcattcgt ctcagacgtt
    15001 tttgcggcct tcgcggaatt tcctgccgcc gttgccgagg aagctgcact gccggcgctc
    15061 gaggctgcgc tcgtttctga tgattttgcc gccacttttg aagccgacgc atcccgggct
    15121 gaggtggcag cttctgacgc tttcgtggtc gcggtggatg cagatgtggc cgcagatttt
    15181 tgtgacgctg cggcattcgt ttctgacgtt ttcgccgcac cggcactggt ggctgccgcg
    15241 ctttttgaag actctgcggc ggcagcactt tttaatgctt cagtggcctt tgttgatgcc
    15301 gttcctgcgc tggaagatgc tgactgagcc gacgacgcgg cctgtccggc tgacgtgctg
    15361 gctgcacgtg ctgagcctgc agcatcagtc gcacgggttg ccgcctcacg ggctgatgtg
    15421 ccggcatcgc tggctgactt cttcgcggct gctgtgttct gcgccactgc ggacgcgtta
    15481 cgcgccacct cttccaccat cagttcaaag cggcgcagtg cctccggacg gacatcatcc
    15541 tccgtcatgg caccgagaaa atcattcagc gtacccggtt gtgagtcttc atacacggtg
    15601 atggtcccgg catgcgatgg cgggaagcct tccaccaaca gaataacgct gtactgaccg
    15661 tactcaacgt ccatgctgta acgcccggct tcatccggat tttctgaggc caccgtgttc
    15721 gccaccaccg tggtgctgtt acgttttgct ttcagctgga ttgtgcagtt ctgtaccggt
    15781 ttacctgcgc cgtctttcag tacacctgaa atctttactg ccatattcac cccacaaaaa
    15841 agcccgcctg aaccggcggg ctgtcataac actgtgttac ctggctaatc agaatttata
    15901 accgacaccc acgatgaaac cgtcagtgcg ccagtcgcta ctgccggagc cttcataagc
    15961 aatatcaatg gccacggatt cggtcgggtt aaactgcacg ccagctcccc acgccagaga
    16021 cgtgttgctg tggcgaccgt catcacttcc ggtcagcaca tcgtgcgttt tccccttgtt
    16081 gtcagttacg cggagataat ccccggagaa agtcgacaca cggctgtaag ccacacccgc
    16141 catcgcatac gcgctgaacc attcattcac gcgcacagac ggccccacca tcacgctgaa
    16201 ccagcggtta cgcacggaat cctcatgcca gcgggtatcg ctgtaatgcg ttttttgctc
    16261 atcttcagcg ttggcataac tgaatgacgt aatcagcccc agcgtgtccg taaactcata
    16321 acggtatttc acgttaatcc cgttcagatt atcgctgccg ggagcgttcg tacgggcatg
    16381 aagataccct gcgctcagtg tggcctgctg ctcagacgcc catgcaggcg caccggatac
    16441 ggccagacag atggctgcgg acaaaatggc tgcacaaact ttacgcataa ttacctctcg
    16501 cttttctgca ataaaaaagg cgccatttct ggcgcccgta tctgggttat aaaattcagc
    16561 tgatactgat gcctgcggtg gctttcttca tcaccacaac cagcaaatcg ctgatacttg
    16621 ctgtgggata ccagttattt accagccatg ctgacaccga aaactccagc gtcatgtggc
    16681 cgcgaccagc tggcatatca ataacaccac tgtaaatcag cgtattatcc agcgcggtac
    16741 ggttataaat ttcagcaccg tttttccgta ctatcaggcg gcatgacgaa taaatatcgt
    16801 tattctcccg ctcatgttta gcgccgctga atgccaccgc cggaataaca atctgccggt
    16861 caaacggctg atcgtcataa accctgacgg tgatggtccc tgatggccac cgttccggtg
    16921 cacgggagtc ccgggggaaa gctttgccca ctgttttaac gagatcgcct tcaatctggt
    16981 tggcggacaa ttttcccaga acccgacagt tctcgttaat cgtgacgttg ttgagcgtcc
    17041 cggagttcgc attcacgtta ccgctgatat cggcattttt cgccgtcagc cgcccgtccg
    17101 gtgtcaggga aaatgccgga ggattaccgc cgctggtaat ggtgggggcc gtcaggcgtt
    17161 tcaggaacac gtcgttcatg aatatctgat caccctgacc aacaaacatc ggttttgtgt
    17221 tgccatttgc aggattaatc atcgcaatcc tgtctgccgc cagcagcacc tgactctgca
    17281 tgccgtcggg ggtgttctca ataccggcac cgatacccgc aatataaagg cgtccgtcct
    17341 gcatctgctg cagcttcaca gcccacatgc tgttcagatt attatttgta tcaacctgaa
    17401 ccttctgtat ctgctggatc gctgcactct ggtcttccag tttcttattg acggtctgtg
    17461 tgatttcatt gctgacatcc gtaatggatg tcctgatttc agccaggtca ggcgcaagct
    17521 gaccgttatc aatctgcgtc cacagctcct gagccagatg ggttttccct atctcgcctt
    17581 tgaaaaaatc cagatagccg gatgcgtcat cactcggctg accaacagcc tccacgaatg
    17641 ccgatttgcc aacggtgttc acactgcgga tataaaagta ataatcatgg cccggtttga
    17701 tattgatact ggcagctatc cagtacagcc ccgtaccaag ataacgcgcg ctggtttcaa
    17761 cctgcctgat atccgcaatc cgcttttccg agaaccagaa ctcaaactgt accgtcggat
    17821 cataaaccgc aagatgcggc gtggcggtta tctgaaaata ccccggcgtc agctcaatcc
    17881 gcgacggtgc taccggtgcg gcaatccgga acgataccga tgccggatcg ccctgctgtc
    17941 cccacgcatt taccgcccgg actgtcagcg tgtaccgccc cagcgccagt tgcgtgaagc
    18001 ggtatgtggt ttccgtcgtc cgggccgtgc tgaccagccg ctcactgccg tcatccgctg
    18061 ttacggtcag acggagcagg aagctcacgc ccttcaccac cttcggcgta tcccagcggg
    18121 ccagtacctg atactccccg ctgtctgcgg tgacttcggc agtcaggtgc tgcactgctg
    18181 gcggcgtgac accattcacc gtgccgctct ggtcgccgtc aaagtgcgcc ccgttatcca
    18241 cgatggcttc tttttccggt acatgctgca cggcggtgat ggcatacgtg ccgtcgtcgt
    18301 tctcacggat actcacgcag cggaacaggc gctggcgcag cgtcggcagc ttcagccccc
    18361 acacgctgta tccggcaacg ccgtcaggaa cccggctcac ttttaccttc acgccgtcgg
    18421 tgacggactg gacctccacg ctgaccggat tgccacttcc gtcaaccagg cttatcagcg
    18481 tggtaccgga ggatggcagc gtgatttcac ggtcgagcgt cagcgtccgg gtctggctgt
    18541 tcaccgccag cacgcgcccg ccgatgctga taccggcata gtcatcatca cagatttcaa
    18601 tgacatcgcc cggtacatgg cgaagccctt cagcacccac gctgaagtcc acggtctgcg
    18661 tttccagcag ttccgtttta atcagccaca gcccggcgcg gtgtgcctgc ccccggctgg
    18721 tacagccaaa ggcatccatc ttcgtgacgt tacgaccgta acgggcaatg gcctgcgtgt
    18781 cctccacaag ctctgtcgcc gtctcccagc cgttattcgg gtcaatccag ttcacctcaa
    18841 cggcattatg gcggtccttc agggcgctga agctgtagcg gaacggcgcg ccatcatccg
    18901 gcatcaccac attactgcgg ttataggtcc acaccttatc cgatggccgg tcctgcacga
    18961 acgtcagcgt ctgcccgttc cataccggca tacagcgcat cgccgagcag aaatcgctga
    19021 gcacatccca cgccttgcgc tgtgtggtca gccaggcatt acaggtgatg cgcggctccg
    19081 tgctgccaaa gccgtccggc accgactggt cgcagtagtg gccgataaca tacagcgccc
    19141 atttatccac atctgccgca ccaagacgtt tccccatgcc gtagcgcggg tgggtcagca
    19201 tatcccacag acaccaggcc atgttgttgc tgtatgccgg tttaaacgtt ccgtcccaga
    19261 taccgctgta ttgccgcgtc tgcgggttat aattcgacgg cacctgcaga atacgcccgc
    19321 gcagatgata attacggctc acctgctggc tgccgaactg ctccgagtcc acctgcacgc
    19381 cgaccagtgc cgtgttcggg tagcactgtt tcacatcgat aatttcggtg tatgacgacc
    19441 agagcgtttt gttctgcagc tggtctgtgg tgctgtccgg cgtcatcctg cgcatccgga
    19501 tattaaacgg gcgcggcggc aggttatcca ccaccactga ggccagatac tgcgacgtgg
    19561 ttttgccctt aatggtgatg tctttttctg tcacccagcc accgttacgt tgtatctgaa
    19621 ccagcaggcg gacttccgac ggattcctgt ccccctttga ggtggtttcc accagtgcct
    19681 gcacaccgaa ggtaaagcgc agacggtcga tatttgcaga cgtgatggtc cgggtgatcg
    19741 gcgtgtcata tttcacttcc gtacccagca ccgtctcgga gccggaggat tcaaatccct
    19801 ccggcggagt ctgctcctgc tcacctgccc ggaacaccac cgtgacgccg gagatgttgg
    19861 tattcccctc actgtccagc actggcgtac tgttcagcag cacgcttttt aatccatcca
    19921 ccggaccttc aatcggccct tcgctgatgg catcgatcac actcagcaac tgcgtggact
    19981 tcaggttgtc cttcgcttcg cgcggagtat gccccttact gctgccttta cccattcctc
    20041 acgctccata aacgacaaaa ccgcccggag gcggtttcac ataaacgttt ttcatcagcg
    20101 accaatcacc acaacctgac caccatcccc ttcgtctgcc gtgctgatct cctgagagac
    20161 cacccgcgac cccacgcgca tttcaccgta cagaacgggc agaacattgc cctgggcaac
    20221 catgttatcc agtgaggaga aataggtgtt ctgtttgccg ttatctgtac ttgctgccgt
    20281 gggcgtcctg gctttcggtg ccagcatctg cgccacacca ccgagcacca tactggcacc
    20341 gagagaaaac aggataccgg tcataccacc ggccccaatg gctgcccccc atgctgcaag
    20401 ggtggctccg gcagtaaaga atgatccggc aatggcggca gcccccagaa caatctggaa
    20461 tacgccccct gacttggccc cggcgactct gggaacaata tgaatcacag cgccatcagg
    20521 cagagtctca tgtaactgcg ccgttaatcc ggacgtgctg acatcccgcc cggcaatccg
    20581 tacctgatac cagccgtcgc tcagtttctg acgaaacgcc gggagctgtg tggccagcgc
    20641 ccggatagct tcagcccccg ttttcacacg aagatcgatg cggcggccaa atcgttgcaa
    20701 atccccgtaa aggcagatgc gtgccattcc cggtgacgcc agagggagtg tgtgcgtcgc
    20761 tgccatttgt cggtgtacct ctctcgtttg ctcagttgtt caggaatatg gtgcagcagc
    20821 tcgctgtcac cacagtaaat ggcggcatga ttcggcaccg atgaaccaaa acagcacagc
    20881 agcacatcgc ccggttgtgc tgatgacaac ggcacctgat acagccccgt cgcctccaga
    20941 ttatccagat agagattctg gccgttacgc caccagtcat cctcacgata aaagtccggc
    21001 atctcaatcc ccgccagatg ataagcatcc cggaacagtg tgtaacagtc cgtcacaccg
    21061 tgctcaaagc gccgcccggt gagatgcggc acacagcgga acttatgaat cgtcccccgg
    21121 cagaccagcc accacggcaa atcactctgc acctgcagcc gccggtcggc ctcactcagc
    21181 cagggcagac caccggggtg gctgtggacc agcgccacaa tctcaccctg catttctgcc
    21241 tgcagccagt cttccggcga catacggaaa tacgcctccg gctcaccgga gatattcacg
    21301 cagggaaaat atctttcccc ctccggcgtg cttaccacga agccgcacga ctccgctggc
    21361 gcacatcgcc gggcgtgcgc cagaatcgct gattctgtct gtgtcatggg atttactgcg
    21421 aaagtttgtt aatggaaagg aagccgccaa agttgccgac gttattgcgg aacttacaac
    21481 cgctcaggca tttgctgcat ttatccttcg tgatatcgga cgttggctgg tcatattcat
    21541 ccgcgacagc cggaccgcta taaccgcact cgtcaccgcg ataggtccag gtgcaggtgt
    21601 tggccagcat gatacgtccc ggaaaaacag cgccatccgt ttccgtcggc gtggacagta
    21661 caaaggaggc actcaccgcg ctcagttcgc tgcactgctc aatgcgccag cggctgatca
    21721 cctcctgctc cggatcggcg tcactgtttc cgttgacgaa gttcaccgca tccagaaaac
    21781 aggcgtaaac cttacgccgg accaccgttc cgccgaccag actctgcaga tcttccgcca
    21841 tcccggtgac cataccgtac aggttagaaa ccgtcagcgt ggggcgcgta ctggtgcctt
    21901 tgccattcag ttcaaaacca ctcccctgaa tgggatacgg ctgatactgt cgcccctgcc
    21961 aggtgaccgg ctcacctttt tcgttctgct cattacagaa aaaataacgt tctccaccga
    22021 cctctgtcag gtcgatttcc cagagcacca cgctggccga ctgctccgca cgggtgcatt
    22081 cattcagtgt ttcctgtcgg atatcctgca tcagttcacc acctgttcaa actctgcgct
    22141 gaactcaaca cgcaacatac tgacccgcga cgaccatttt gcgcaggtca cctttatctg
    22201 ccgccactca taaggcggcg tccacagaaa ggatttccag cccccgtgct cagccagaaa
    22261 cgactccagc gccgcggcct cccaacggga gacagacagc gtcacgctgt acgttttcag
    22321 gtcagcgttc agcccggcag gcgctcgctg ggaatagcca tcaccaaagc gcacctttct
    22381 tacggaaggg gccgaagcca catccatacc gggtttcact ttccagcgga aggttttcat
    22441 cgtccacctc cggagaacag gccaccatca cgcatctgtg tctgaatttc atcacgggca
    22501 cccttgcggg ccatgtcata cactgccttc atcatctgtg gacctggcag accattcgta
    22561 ccgtcgttct gaatcaccac gtgattgttc tgattaaaat taatgccttc agcccgccgc
    22621 atctgcgccg gacttccggc accgcccaca taaccacctt ccgcatagcc ccgcatcagg
    22681 cggtacaggt tgccgacacc aatccggctg gttgcctcct tcgtgaagac aaattcacca
    22741 cggtgaacaa tccccgctgg ctcatatttg ccgccggttc ccgtaaatcc tccggttgca
    22801 aaatggaatt tcgccgcagc ggcctgaatg gctgtaccgc ctgacgcgga cgcgccgcca
    22861 ccaacagccc cgccaatggc gctgccgata ctcccgacaa tccccaccat tgcctgctta
    22921 agcagaattt ctgtcatcat ggacagcacg gaacgggtga agctgcgcca gttctgctca
    22981 ctgccggtca gcatcgccgc catattctgt gcaataccat caaaggtctg cgtggcagca
    23041 cttttaacct gcgacatact gtccgtggcg ctctcttccc actcactcca gccggacttc
    23101 aggcctgcca tccagctccc gcgaagctgg tcttcagccg cccaggtctt tttctgctct
    23161 gacatgacgt tattcagcgc cagcggatta tcgccatact gttccttcag gcgctgttcc
    23221 gtggcttccc gcgctgcctg ccggtcagtc agcccccgtt tttttgcatc aatggcggcc
    23281 cgttttgccc gttgttgctg tgcgaactta tccgcctgct gcgccagcgc gttcaggtgc
    23341 tcctgatagg tgaccttgtc gccaagtaca gccagctggc gtttgtactc cagcgtctcg
    23401 tctttatgcg ccagcaggga tttctcctgt gcggacagct ggcgacgttg tgccgcctcc
    23461 tccagtaccg cgaactgact ttctgccttc cacaaatccc ggcgctgctg gctgattttc
    23521 tcattcgctc cggcatgctt ctccagcgtc cggagttctg cctgaagcgt cagcagggca
    23581 gcatgagcac tgtcttcctg acgatcgccc gcagacacct tcacgccttt cggctttttc
    23641 agcgtcgctt catagtcctt tttcgccgcc gccatcagcg tgttgtaatc tgcctgcaga
    23701 attttcccgt ccttcagtgc cttgttcagt tcttcctgac gggcggtata tttctccagc
    23761 ggcgtctgca gccgttcgta agccttctgc gcctcttcgg tatatttcag ccgtgacgct
    23821 tcggtatcgc tctgctgctg cgcatttttg tcctgttgac tctgctgctc agccttcttt
    23881 cgggcggctt caagcgcaag acgggccttt tcacgatcat cccagtaacg cgcccgcgct
    23941 tcatcgttaa caaaataatc atccttgcgc agattccaga tgtcgtctgc tttcttaaac
    24001 gcagcctctg ccttaatcag catctcctga gcggtatcag gacgaccaat atccagcacc
    24061 gcatcccaca tggatttgaa tgcccgtgct gtcctgtctg cccaggtttc cagcgtgccc
    24121 atgttctctt tcaggcgtcg ggtctggtca tcaaaccctt tcgttgcggc ctcgttcgcc
    24181 gcctgcaatg ccccggcttc atcgccggaa cgctgcaact gagcaacata cgcaatctgc
    24241 tccgccgtca cgttatggaa ctggcgtgcc atcgccgtca gccccgacgt cgggtctgtg
    24301 gtcagcttcc cgaaggcttc agcgaccttg tccacctcca cgccggatgc agaggagaaa
    24361 cgcgccacac tctggctgat ggacgcaatc tgagcctcac cgcttacccc cgccttaacc
    24421 agtgcgctga gtgactcgct ggtctggtta aacgtcagcc ctgccgcctg cccggctctg
    24481 gacaggacca gcatacgatc tgccgtcagt cccgcctgat tgccggaaag gaccagcgtt
    24541 ttgttgaaat cggacagggt tgagttgccc tgataccagg catacgccag cgctccggtc
    24601 gccaccgcca gcgaggtggc ccccaccatc ggcagggtga tcgcaccggc aagcccccgg
    24661 aacatgggga tcatcccgcc gaaggagtcc ttcacctgac ccccctgttg cagcaggatc
    24721 agccacggac tttgcccgcc tgcaagctgc gtggccacgt cagtgaactg cgcaggcagc
    24781 atacgcatgg cggctttata ctgcccgacg gaaattcccg ctttctgtgc agccagcgcc
    24841 tgccggttca tcgactgttc aacgactgcc gctgtttttt tcgcatcact ttccgtaccg
    24901 gaaaaatgac gcctgactct ggccatctgc tcgtcaaatc tggccgcatc cagactcaaa
    24961 tcaacgacca gatcgcctac cggttcagcc ataccggact cctcctgcga tcccttctga
    25021 tactgtcatc agcattacgt catcctccgt catgtccgcc acatccgggg aagcggggat
    25081 aacttcattc ccgtccgggc caaagcggac gcctccggca agccctgccg ctttctgcat
    25141 cagcacatca tcttcaggct cttcgtcagc ctcgcgccgg ttcagcagac tgaaatccag
    25201 cggatgcata tccggatcgc tgaaaaacag gctgagcacg gtgtacgtca gcccggaaaa
    25261 gtgcatatcc agcagaacat catgaaaata atgggtactg taaaagcggt gccagtcggc
    25321 atactccgtg gatgacatcc cggcaagcat ggcacgccag tcgggtcgcc ccatctcacg
    25381 cgccagtttc agggcaaaac tcagctcacc gtcgaacact ttcccgcaga aacaggctct
    25441 gcgggcccgg cgtcctctgt ctgttcaggg gcattattca ccacaaactc atacatacca
    25501 gacagccggt acaccacgtt ttcagcatga gaaattgcct ccgtgggcca ggtggtaagc
    25561 acttcctgct caatctgttt aacggcttca ttcatggacg gcatctgcgt cttctgcgga
    25621 tggttatgcc acagggacat cgccaccaga aacgcgccgg ttctgatggc gtcttccaca
    25681 gtaaacttcc ggttgctgtc tgactccgcc tgttctgcct gccgtttcat cagggcgaga
    25741 tgctcaatac gctgcagggc tgacagttca gaaagcgtga cggtcacacc gttatgttca
    25801 aatgattcgg ttttcaggaa catcgctgac tctccggatt aactggcggt gacgttgatt
    25861 tctgcaaccg cagcaaactc accattaccg gatacgaccg gaatgttgac cttgcctgca
    25921 gcaacacctt tcacggtgat ggtcatacca ctgaccgaca cggtggcttt tgttttatcc
    25981 gcagacaccg cacggaagct cttgtcggtt gcgccctccg gctggaatgc cacggtcagc
    26041 gtggtgctct gccctttcac tacggaagca ctggcgggtg tcaccgtcat gccggttgtc
    26101 gccgtcaccg tgctgcgatc ttctgccatc gacggacgtc ccacattggt gactttcacc
    26161 gtgcgggtga tcacttcctt cgccgtcacc gccttaccga tactgctgac ccagccacgg
    26221 aacacatcga ccgtgccgtt cgggaagcgg attttatagg cacgagtatc gccttcatta
    26281 aaccacgcca gcagcgcctg ctgcccctgc tctccgggca tccacgccag cgtgaagctg
    26341 gtatctccgg cagatttctg cccctgcccg gtcgcagtcc agtctgcatc ttcatcatcg
    26401 agatagctgt cgtcatagga ctcagcggtc agttcgccgg gcgtcaggtc tttaactttt
    26461 gccagacgcg accagtcaac gtctgaaagc ggattcgcat aagggtcacc gctcccctta
    26521 taaacccaca gggtggtccc ggcacctttc accggcattg taggatttgg tacaggcata
    26581 gcgtcctcac atttcatagg taatgacata agtcagatcg gctgaactcc acaggcccgc
    26641 atcatcgtcg cgccggtagt catagccgct ggccaccata ctggtgatca aatctgacag
    26701 tgccgggata tcgctcatca ccggataaat ccgggactcc atccacgcat ccagctctga
    26761 atccggcacc tgagcaggca ggaaaacttc gatatgcagc tccgcctgcc aggtatcgct
    26821 gtccagctct tcgcccgtgt attcagcgcc ggtgagataa acggcaactg ccggaaaatc
    26881 cgcctcatca aaaacagcgg ggcgaccatc aaaaagcgtc gccccggtgt catgcttctc
    26941 cagtgcatcc agtacggctg cacggagttc agtatgtttc atcgctttat taccattctc
    27001 agttgatgct gcagcgcata gcccagctct ttcggaagac gttcacgccg tatccgttca
    27061 atattctgtt taaacgccgt ggtaagcggc accgccatcg ggattttcac cacatcaatg
    27121 gggtaacggt ttttcccggc cacacgctgc atgacatgcc accggccatt tttcagttgc
    27181 tgaataaacg cgccgggaat acgacggttt cccaccacaa gcacgctgcc gccacctttc
    27241 agggatgaac gctgcccctt tttacgacgt ctgcggcggg aaaggacaac ccgcgcgtta
    27301 cccagcttga ttacgggcaa atccccccgg ttaaccctga ttctggcctg cggatttttg
    27361 accgtggccc ttttcagcct ggccctttcc tttaccagtt tccggcgtac ctttgtctca
    27421 cgggcaacct gtgccaccga ctgcgatatc gcggatgaag caacgcggtt aatggccatt
    27481 gcggcggcac cgggcaccgc cgttctgctg atacggctga ggttttcaac ggcctgctca
    27541 agacctttta tggccataca tccccctttc agcggcgacg gttaacggca ggcggtacgc
    27601 cccgcccaag ccagagatga cagcttccgc catcatccgg cgaaatccgg tctatccaga
    27661 agttttcctc accgatggtc agcgtgtcgc cgcgccgcag ctgccgcaca tcatcagtcc
    27721 ggacaaacag ggacgggctg gagccttcaa cgcgcacgcc ctgtccggca tagctgatat
    27781 tttcagggtc atcaaaaaca ccacgtatca cagcaccgga ctgctcaccg gatgtcatgg
    27841 tggctgacgt tcccatgtac ccgcgtatcg tttcatcggc gcaggcaatg gcagcatcga
    27901 acaggttatc gaaatcagcc acagcgcctc ccgttattgc attctggcca ggccgcgctc
    27961 tgtcatttca gctgccacac cggcagagac acggaacgcc gttcccggca gcacaaatgc
    28021 cacaggctca tcccgcgtgg cgtgaagtgc atcagtatgc agcgtcacca gtgccacgac
    28081 cgtgaccaga gcagccgtat caatcacggt atccggctgc gctgatacca cctcattttc
    28141 atgtccggtc agcgcatttt ccgggctgac agatgtgtcc tgaccggcag catcatccgt
    28201 gtcatcaagc tcctcttcca gctctgccac acggagcacc agttcttctt tcgtccccgt
    28261 caggctgaca tcacggttca gttgctcacc cagcacctga agacgggcaa tcagttcatc
    28321 tttcgtcatg gactcctcca cagagagaaa atggccccga agggccatga ttacgccagt
    28381 tgtacggaca cgaacgcatc aggatcagcc agcagcatca gcggtgctga ctgaatcatg
    28441 gtgaactcac gcgccggatc gccggtggtc acccagtttt tcgggtagcg ggcagaggcg
    28501 ttaatacctt cgcgctgtgc gtccgcatcc tgaatgcagc cataggtgcg cagaccgcgt
    28561 gcctgagtgt tccccagcac catcgtgttg tccggcagga agttcttttt gacgtcgttt
    28621 tccacgtact gtccggaata cacgacgatc gccgtatcgc catacatccc cttataggac
    28681 accgcttcgc ccaggtcttt taccgctgtc tccagctcgg aatgagagcc gcgacgggta
    28741 tccagcttct ccttgacggc tttgaaggaa cggaacagcg cccagccttt cggatcaaac
    28801 acgatgatat tcaccacgcc gctggcgttc agcgcatagg cttcgatatc gtcggtcggg
    28861 tcatacgtgg acttgtcacg cttgctccac tccgtaccac cggactgtgt gatgttgttc
    28921 gccgcactgc ggcccatatc cacctcaacc ggatcgaagg cttcaccggt catggtgtat
    28981 ttgcccttaa gcacagcaga aactgcctgc atctcttcga cctgagcaat ggccagctct
    29041 tcgtctcgca tgttctgcat gatgatgcga cggcggcggt aagccgggtc cgccagattc
    29101 tgcggatctt catccggcag gcgacgcagg gtcatctgcg gattcacctc atgcttgggt
    29161 ttgacatatc ccggcgtaaa ttcagaggtg gagccgccac gggaacggat aacctcaccg
    29221 gaaacaatcg gcgaaacgta cagcgccatg tttaccagtc ccggaatttg tgagagatag
    29281 actttctccg tggtgaaggg atagctctca cggaaaaaga gacgcagaaa cagcggatca
    29341 aacttaaatt tctgctcatt tgccgccagc agctgggcgg ttgtgtacat cgacataaaa
    29401 aaatcccgta aaaaaagccg cacaggcggc ctttagtgat gaagggtaag gttaaacgat
    29461 gctgattgcc gttccggcaa acgcggtccg ttttttcgtc tcgtcgctgg cagcctccgg
    29521 ccagagcaca tcctcataac ggaacgtgcc ggacttgtag aacgtcagcg tggagctggt
    29581 ctggtcagca tcaaccgcca gaatgccaac ggcagtaccg tcggtggtgc catcccacgc
    29641 aaccagctta cgggtggagg tgtccagcat cagcggggtc attgcaggcg ctttcgcact
    29701 caatccgccg ggcgcggttg ccgtatgtgc cgggtcactg ttgcccagcg gctggtaatg
    29761 ggtaaaggtt tctttgctcg tcataaacat cccttacact ggtgtgttca gcaaatcgtt
    29821 aacggcatca gatgccgggt tacctgcagc cagtggtgcc ggtgccccct gcatcagacg
    29881 atccagcgca gtgtcactgc gcgcctgtgc actctgtggt gcagctgcca gaatgcggcg
    29941 ggccgtttcc acggtcatac cgggggtttc ggccagcaca cgtgcctgtt cttcgcgtcc
    30001 gtgagcttcc tcacagttga ggatccccat aatgcggctg ttttctgccg caaccgcagc
    30061 ggtgatctgc gcgttcacgt ccggctgcgc cgcgctggcg ttttcgccct ccgtcgcttg
    30121 caccacgtca gtaacgtcag cctgcgaagc agtggctgaa acagttgttg attgagtctc
    30181 tttggtcatt cgccctcctg agagacggga tttacgtgca tccagtgcat cacgcataac
    30241 ggtgatcgca tcggtgctgt tgacaagttc atcagccagt ccggcatcaa tggcctcctg
    30301 accgctgtac actgcagcct cggtatccag cacagcctgc acggacaggc cggtatatgc
    30361 cgacaccttc tgcgcaaaca tccggcgggt tgcatccatc cgggactgca gtgtctcccg
    30421 gacatcaccc ggtagatggc tgtaggggtt gccatccacc ttatggctgc cgctgtaaat
    30481 cagcgtgatt tccacgccct gtttctccag cgcagcgccg taattactgt gagccatcat
    30541 gacgccgatg gagcctgtcc gggcggtctg cgtgaccaga cgccgggagg cggcgctggc
    30601 aagcagctga cctgcactgc agttcatgtc gttggccagc gcccataccg gctttatgtc
    30661 acgcacacgg gcgatgatgt cagcacagtc aaatgctccc gccaccatcc cgcccggtgt
    30721 gtccatatcg agcagaatgc cgtccaccat cggatcgctg gcagcctgtt gcagacgagc
    30781 gataatgccg ttgtaaccgg tcattcccga atacggctgc agcgcccgcg tccggctgac
    30841 cagcgtaccg gacaccggca gcacggcgat gccgttcatg acctgataac tgcgggcctg
    30901 tcgtggtccg tcatcatcac cggataacgc cagcgccgcg ggtgcctctc cggcagtcag
    30961 gctgtcgccg gatactgcat ccgtcaggcg actgatccca agctggcctg caagcgcaca
    31021 aaagaaaacc cgcgcatagg cgggttcaag catcagcggc tcattaaaag ccatgctggc
    31081 aatatgcggg agattacgca gctctgctgt cactcttctc ctcctctgtt gattgtcgca
    31141 gtccggattc aaatgccgca gccgcccagg cgggcggttt aagaccggct gcacggcgct
    31201 ccatcgtttc acggacctgc tgggcaaaaa tttcctgata gtcgtcaccg cgtttcgcgc
    31261 actctttctc gtaggtgctc agtccggctt ctatcagcat caccgcttcc tgaacttctt
    31321 tcagaccatc gatggccata cgaccggagc ctatccagtc gcagttcccc caggcactgc
    31381 gggcttcctg aaagctgaag cgcgcttttg aaggtaacgt caccacgcgg cgaacgatgg
    31441 cctcttcaag ccagcacaga aacatctggc tcgcctgacg ggatgcgacg aattttcgcc
    31501 gccccataaa gtacgcccac gactcgttcg cactggcccg tgccgtggag tagctcatct
    31561 gggcgtaatt ccgggaaagc tgctcatacg agacacccag cccggcagcg atataccgca
    31621 acagtgactg ctcaaacacg gagtagccgt tatccgtatc ctgagccgtc tgcaggttca
    31681 gtgagtcccc cggcatcagg tgcggcactt ttgcgcctcc cagacggacc ggtgctgcgg
    31741 cgtaatacgc ggcaatttca ccaatccagc ccgtcagcct ttcccgctgc tcctgactgt
    31801 tcgcgcccag aataaaatcc atcgctgact gcgtatccag ctcactttca atggtggcgg
    31861 catacatcgc cttcacaatg gcgctctgca gctgcgtgtt ctgcagcgtg tcgagcatct
    31921 tcatctgctc catcacgctg taaaacacat ttgcaccgcg ggtctgcccg tcctccacgg
    31981 gttcaaaaac gtgaatgaac gaggcgcgcc cgccgggtaa ctcacgaggt atccatgtcc
    32041 atttctgcgg catccagcca ggatacccgt cctcgctgac gtaatatccc agcgccgcac
    32101 cgctgtcatt aatctgcaca ccggcacggc agttccggct gtcgccggta ttgttcgggt
    32161 tgctgatgcg cttcgggctg accatccgga actgtgtccg gaacagtcgc gacgggctgg
    32221 tatcccaggt ggcctgaacg aacagttcac cgttaaaggc gtgcatggcc acaccttccc
    32281 gaatcatcat ggtaaacgtg cgttttcgct caacgtcaat gcagcagcag tcatcctcgg
    32341 caaactcttt ccatgccgct tcaacctcgc gggaaaaggc acgggcgtct tcctccccga
    32401 tgcccagata gcgccagctt gggcgatgac tgagccggaa aaaagacccg acgatatgat
    32461 cctgatgcag ctggatggcg ttggcggcat agccgttatt gcgtaccaga tcgtctgcgc
    32521 gggcattgcc acgggtaaag ttgggcagca gggctgcatc cacactttca cccggtgggt
    32581 tccacgcccg caactgccca ccaaatccgc tgccaccgcc gtgataaccg gcatattcac
    32641 gcagcgatgt catgccgtcc ggccccagaa gggtgggaat ggtggacgtt ttcatacata
    32701 aaatcctgca ggtcccctgc gtcgctgtgt catgccggtc tgcacttcca gctccgcaat
    32761 gtattttttc aggtcagaca cggaagtggt cgtaaactcc actcgccgtc cgtctttctg
    32821 taccgttgcc acccgttttc ctgtcatcag gtcatgcagt gccgcacggg cagcggcaag
    32881 ttcttcctgt cgcgtcattc atcctctccg gataaggcac gggcgtaatc tgccagtgtt
    32941 ttcttgttgg ttgctgcacc atcctcttcc tgcaggctcg ccagcagtgc actgagatcc
    33001 agctgccagc gggaaatact gatgcgcagc gccgccagcg cataaacgaa gcagtcgagc
    33061 gcctcattgc gtcgcttttt gctgtcccac agtatttttt tcctgccatc cacccatttt
    33121 tcgacctgct cttcagcagt cagctgctgc gcttcggtca gatcaaaaat atccgggtta
    33181 ttcgggaagt gaacggcacc gggaagcggt tcatcccctt ccggcgtcag tgtgaagcgg
    33241 ttataaatct gctctttcgc ggtatccgta ccgatttcgg taaggtaaac cccgtttttg
    33301 tttcgcttac gtggcatgct ggccacaggc ttaccgtaga cggatgcccc tttaatgggg
    33361 atcacccgga acagcccatg ttttttcgag cgttcataca caatggtcgg gtcaatcccg
    33421 ccagtatccc agcagatacg ggataccgac atttctgcac cattccggcg ggtataggtt
    33481 ttattgatgg cctcatccac acgctgcagc gtctgttcat cgtcgtggcg gcccataata
    33541 atctgccggt caatcagcca gctttcctca cccggccccc atccccatac gcgcatttcg
    33601 tagcgatcca gctgggagtc gataccggcg gtcaggtaag ccacacggtc aggaacgggc
    33661 gctgaataat gctctttccg ctctgccatc acttcagcat ccggacgttc accgattttc
    33721 gcttcccacg tctcaccgag cgtggtgttc acgaaggttt tacgttttcc cgtatcccct
    33781 ttcgtcttca cccagtcttt gacaatctgc acccaggtgg tgaacgggct gtacgccgtc
    33841 cagatgtgaa aggtcacact gtcaggcggt tcaatctctt caccggatga cgaaaaccag
    33901 agaatgccat cacgggtcca gatcccggtc ttttcgcaga tataacgggc atcagtgaag
    33961 tccagctcct gctgacggat gacacaggcg ttatgctcgc agagataaaa cacgctggag
    34021 gggtcatccg gcgtccattt gaggccaaac ggcgtctctt tatcgccaaa tttaaggtac
    34081 tgctcctccc cgcagtgcgg gcaggcaaca tgaaaacgca taaaatgcgg ggattcactg
    34141 gctgcacgct caatctggca ggtgcctctc actttgggcg tggagccacg gatggacttt
    34201 ggccagaccg agccttcaat acgcttgtcg cccaggaacg tcggagagcc ttcctgttca
    34261 atatcatcat caaaggcagc aagttcatca taacccgcca catccactga cttttcacgg
    34321 tagttttttg ccgctttacc gcccaggcac cagaagccac gcccattggt gaaacgcttc
    34381 atggtgagcg tgttatcccg gtgctttttg ccataccacg gggccagcgc cagcagcgac
    34441 ggaatatcac gtatggtcgg ctcaacgtgg gttttcataa agttctcggc atcaccatcc
    34501 gtcggcaacc agataagggt gttgcgctgc ttatgctcta tgaagtaggc ataaacaccc
    34561 aacagcattt tggaataacc gacacgggca gacttcacca cattcacctc gcggatgtag
    34621 tcgctgccca tcgcattcat gatggcccgc tgaaagggca gtgtttccca gcgcccttcc
    34681 tggtatgcgg attctttcgg gagatagtaa ttggcatccg cccattcaac ggcggtctgt
    34741 ggctccggcc tgaacagtga gcgaagcccg gcgcggacaa aatgccgcag cctgttaacc
    34801 tgactgttcg atatattcac tcagcaaccc cggtatcagt tcatccagcg cggctgcttt
    34861 gttcatggct ttgatgatat cccgtttcag gaaatcaaca tgtcggtttt ccagttccgg
    34921 aaaacgccgc tgcaccgaca gggggatccc gtcgagaata ctggcaattt cacctgcgat
    34981 ccgcgacaac acgaaagtac agaatgcggt ttccaccact tcagcggagt ctctggcatt
    35041 cttcagttcc tgtgcgtcgg cctgcgcacg cgtaagtcga tggcgttcgt actcaatagt
    35101 ccctggctgg agatctgcct cgctggcctg ccgcagttct tcaacctccc ggcgcagctt
    35161 ttcgttctca atttcagcat ccctttcggc ataccatttt atgacggcgg cagagtcata
    35221 aagcacctca ttacccttgc caccgcctcg cagaacgggc attccctgtt cctgccagtt
    35281 ctgaatggta cggatactcg caccgaaaat gtcagccagc tgctttttgt tgacttccat
    35341 tgttcattcc acggccaaaa acagaga
//
LOCUS       DAJOSY010000036        35058 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJOSY010000036 DAJOSY010000000
VERSION     DAJOSY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.036"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            47..1300
                     /locus_tag="OHN27_001891"
     CDS             47..1300
                     /locus_tag="OHN27_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2286339.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1697..2032
                     /locus_tag="OHN27_001892"
     CDS             1697..2032
                     /locus_tag="OHN27_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCQ2286340.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETTTTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            2125..2208
                     /locus_tag="OHN27_001893"
     CDS             2125..2208
                     /locus_tag="OHN27_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286341.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2308..3129
                     /locus_tag="OHN27_001894"
     CDS             2308..3129
                     /locus_tag="OHN27_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCQ2286342.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVARSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3168..3497)
                     /gene="mdtI"
                     /locus_tag="OHN27_001895"
     CDS             complement(3168..3497)
                     /gene="mdtI"
                     /locus_tag="OHN27_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCQ2286343.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3484..3849)
                     /gene="mdtJ"
                     /locus_tag="OHN27_001896"
     CDS             complement(3484..3849)
                     /gene="mdtJ"
                     /locus_tag="OHN27_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCQ2286344.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            4261..5295
                     /gene="tqsA"
                     /locus_tag="OHN27_001897"
     CDS             4261..5295
                     /gene="tqsA"
                     /locus_tag="OHN27_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCQ2286345.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(5320..6708)
                     /gene="pntB"
                     /locus_tag="OHN27_001898"
     CDS             complement(5320..6708)
                     /gene="pntB"
                     /locus_tag="OHN27_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCQ2286346.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(6719..8251)
                     /gene="pntA"
                     /locus_tag="OHN27_001899"
     CDS             complement(6719..8251)
                     /gene="pntA"
                     /locus_tag="OHN27_001899"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCQ2286347.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCTCSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8775..9719
                     /locus_tag="OHN27_001900"
     CDS             8775..9719
                     /locus_tag="OHN27_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2286348.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            9905..11287
                     /locus_tag="OHN27_001901"
     CDS             9905..11287
                     /locus_tag="OHN27_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2286349.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            11324..12046
                     /gene="folM"
                     /locus_tag="OHN27_001902"
     CDS             11324..12046
                     /gene="folM"
                     /locus_tag="OHN27_001902"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCQ2286350.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(12043..12378)
                     /locus_tag="OHN27_001903"
     CDS             complement(12043..12378)
                     /locus_tag="OHN27_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCQ2286351.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            12507..13226
                     /gene="rstA"
                     /locus_tag="OHN27_001904"
     CDS             12507..13226
                     /gene="rstA"
                     /locus_tag="OHN27_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCQ2286352.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            13230..14531
                     /gene="rstB"
                     /locus_tag="OHN27_001905"
     CDS             13230..14531
                     /gene="rstB"
                     /locus_tag="OHN27_001905"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCQ2286353.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNKATLIVEDDGSGIAPENREHIFEPFVRLDPSRGRSTGGCGLGLA
                     IVHSIALAMGGTVNCGTSELGGARFSFSWPLWHNIPQFTSA"
     gene            14607..15536
                     /gene="tus"
                     /locus_tag="OHN27_001906"
     CDS             14607..15536
                     /gene="tus"
                     /locus_tag="OHN27_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCQ2286354.1"
                     /translation="MACYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(15533..16936)
                     /gene="fumC"
                     /locus_tag="OHN27_001907"
     CDS             complement(15533..16936)
                     /gene="fumC"
                     /locus_tag="OHN27_001907"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCQ2286355.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNINEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEYNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVAALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(17079..18725)
                     /gene="fumB"
                     /locus_tag="OHN27_001908"
     CDS             complement(17079..18725)
                     /gene="fumB"
                     /locus_tag="OHN27_001908"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCQ2286356.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            18924..20099
                     /gene="manA"
                     /locus_tag="OHN27_001909"
     CDS             18924..20099
                     /gene="manA"
                     /locus_tag="OHN27_001909"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCQ2286357.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20200..21708
                     /locus_tag="OHN27_001910"
     CDS             20200..21708
                     /locus_tag="OHN27_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCQ2286358.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(21753..23018)
                     /gene="uidC"
                     /locus_tag="OHN27_001911"
     CDS             complement(21753..23018)
                     /gene="uidC"
                     /locus_tag="OHN27_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCQ2286359.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(23057..24430)
                     /gene="uidB"
                     /locus_tag="OHN27_001912"
     CDS             complement(23057..24430)
                     /gene="uidB"
                     /locus_tag="OHN27_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCQ2286360.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLN
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(24427..26238)
                     /gene="uidA"
                     /locus_tag="OHN27_001913"
     CDS             complement(24427..26238)
                     /gene="uidA"
                     /locus_tag="OHN27_001913"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCQ2286361.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(26629..27219)
                     /gene="uidR"
                     /locus_tag="OHN27_001914"
     CDS             complement(26629..27219)
                     /gene="uidR"
                     /locus_tag="OHN27_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCQ2286362.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(27440..28207)
                     /gene="hdhA"
                     /locus_tag="OHN27_001915"
     CDS             complement(27440..28207)
                     /gene="hdhA"
                     /locus_tag="OHN27_001915"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCQ2286363.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(28319..29347)
                     /gene="malI"
                     /locus_tag="OHN27_001916"
     CDS             complement(28319..29347)
                     /gene="malI"
                     /locus_tag="OHN27_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCQ2286364.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKYGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            29522..31114
                     /gene="malX"
                     /locus_tag="OHN27_001917"
     CDS             29522..31114
                     /gene="malX"
                     /locus_tag="OHN27_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCQ2286365.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            31124..32296
                     /gene="malY"
                     /locus_tag="OHN27_001918"
     CDS             31124..32296
                     /gene="malY"
                     /locus_tag="OHN27_001918"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCQ2286366.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     NNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            32400..33401
                     /gene="add"
                     /locus_tag="OHN27_001919"
     CDS             32400..33401
                     /gene="add"
                     /locus_tag="OHN27_001919"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCQ2286367.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(33435..34475)
                     /locus_tag="OHN27_001920"
     CDS             complement(33435..34475)
                     /locus_tag="OHN27_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCQ2286368.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            34718..34843
                     /gene="blr"
                     /locus_tag="OHN27_001921"
     CDS             34718..34843
                     /gene="blr"
                     /locus_tag="OHN27_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCQ2286369.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
BASE COUNT         8626 a         9087 c         8479 g         8866 t
ORIGIN      
        1 cctatgtata agcctgatct acaggcatat ttagcaagga tttcaagtga gccgtactac
       61 aactgttgat ggcgctccgg caagcgacac tgacaagcaa agcatttctc agccaaatca
      121 atttattaaa cgcggtacgc cgcaatttat gcgcgtcacc ctggcgctgt tctctgccgg
      181 actggcaaca tttgcacttc tctattgtgt gcagcctatc cttccggtgc tttcgcagga
      241 gtttggctta acccccgcga acagtagtat ttcactgtcc atttccacgg cgatgttggc
      301 tattggtttg ctgtttactg gcccgctatc cgatgccatt ggtcgcaaac cagtgatggt
      361 cacggcgcta ctgttggcct ccatttgtac gttactttcg acaatgatga ccagctggca
      421 cggcattttg attatgcgcg ccttgattgg gctttcgtta agtggcgtgg cagctgttgg
      481 catgacttat cttagcgagg aaatccatcc cagtttcgtg gccttttcaa tggggttgta
      541 tatcagcggc aactcaattg gcggcatgag cggacgctta attagcggtg tcttcacgga
      601 ctttttcaac tggcgaattg ctctggcggc aatcggttgt ttcgcgctgg cctcggcgtt
      661 gatgttctgg aaaatcctcc ctgaatcacg ccattttcgc ccgacttcgc tgcgccctaa
      721 gacgttgttt atcaactttc gtctgcactg gcgtgaccgg ggattaccgt tattgttcgc
      781 agaaggcttt ttgctgatgg ggtcgttcgt cacgctgttt aattacatcg gctatcggtt
      841 gatgctctcc ccctggcatg tcagtcaggc cgtggttggc ttattatcgc tggcttattt
      901 gaccggtaca tggagctcac ccaaagccgg aaccatgacc acccgctatg ggcgtggtcc
      961 agtgatgttg ttttcgacgg gggttatgct gtttggttta ctgatgacct tattcagctc
     1021 gctgtggctg atctttgccg gaatgttact cttctcagca ggattcttcg cagcccactc
     1081 agtagccagc agctggatcg gcccccgcgc aaaacgcgct aaaggccagg cctcctcgct
     1141 gtatctgttc agttactatc tggggtcgag tattgccggg acgctgggtg gtgttttctg
     1201 gcataactat ggctggaacg gcgtcggcgc atttattgct ctgatgctgg tcattgctct
     1261 gctggtcggg acgcgtttgc atcgtcgtct gcacgcctga aaaataagtc cggactgcgg
     1321 taaatacccg tccggactta ttgccagctc aaaccaacgt taatagccat cctaaaatag
     1381 acgaagcgcc agccaattcc cgcagcgcgt ctagcgtcat caggattata agtacccaaa
     1441 taaacggatt cattttgctg tgtgtcattt attactgatg cgcagttatt ctactgcttt
     1501 gtaagtagta aaatagttaa cccgatcaag actactatta ttggtagcta aatttccctt
     1561 aagtcacaat acgttattat caacgctgta atttattcag cgtttgtaca tatcgttaca
     1621 cgctgaaacc aaccactcac ggaagtctgc cattcccagg gatatagtta tttcaacggc
     1681 cccgcagtgg ggttaaatga aaaaacaaat tgagggtatg acaatgaaaa aagtattagc
     1741 tctggttgtt gccgctgcta tgggtctgtc ttctgccgcc tttgctgcag agactacgac
     1801 cacacctgct ccgactgcga cgaccaccaa agcagcgccg gcgaaaacta cacatcataa
     1861 aaaacagcat aaagcagcac ctgcccagaa agcgcaggcg gctaaaaagc atcataaaaa
     1921 tacgaaagct gaacagaaag cccctgaaca aaaagcgcag gcagcgaaga aacacgccaa
     1981 gaaacacagc catcagcaac cggcaaaacc tgctgcacaa cccgcagcgt aagttttcaa
     2041 cagtaatgct ggcgcgcccc ctcgcgcctg aaaattacgg tgctaagcgg gtaacgttta
     2101 gcaccgcctt tagcggaggg tagtatggtg ggccgttatc gctttgagtt cattctgatc
     2161 atccttattt tatgcgcact gattgccgcc cgtttttatc tttcctgatt gtagttatct
     2221 gattttactc ccactttcat cccgtcccgt ctatagtatt tatgagggtt tgcttttaat
     2281 aatcataatt acccaccaga gtgtgatatg cgtacaacca ttgctgtagt gttgggtgca
     2341 attagtttga cgtctgcttt tgtgtttgca gataaaccag acgttgccag atcggcaaac
     2401 gatgaggtca gcaccctgtt ttttggtcat gatgatcgtg tgccagtgaa tgacacgacc
     2461 caatcaccgt gggatgcggt tgggcaactg gaaacggcca gcggcaattt atgtacggcg
     2521 acgctgattg cacccaatct ggcattaacg gcaggacact gtttattgac acctccaaag
     2581 ggtaaagccg ataaagcagt ggcgctgcgt tttgtgtcaa ataaaggtct ttggcgctat
     2641 gagatccacg acatagaagg ccgcgttgat ccgacactgg gaaagcggtt aaaagcagat
     2701 ggggatggtt ggattgtacc tcccgcagcc gcgccgtggg acttcggatt gattgtgcta
     2761 cgtaatcccc cttctggcat tacgccgttg ccgttatttg agggagataa agccgcgctt
     2821 accgccgcat taaaagcggc aggtcgtaaa gtgactcagg caggctaccc tgaagatcat
     2881 ctcgatacgt tgtacagtca tcaaaactgt gaagtgactg gctgggcgca aacgtcggtg
     2941 atgtcacatc agtgcgatac cttgccgggt gacagcggtt cgcctctgat gttgcatacc
     3001 gatgacggct ggcaattaat tggggtgcaa agttcggctc ctgccgcgaa agatcgctgg
     3061 cgcgccgata accgggccat ttctgttacc ggttttcgcg acaagctgga tcaactgtcg
     3121 caaaaataat gttcaaacgc tgcccgacag cgcgggcagc gtcttcatca ggcaagtttc
     3181 accatgatca ttccagccaa cagcaagacc aggccaatcc agcctttacg atttaaccgt
     3241 tgaccaaaca agatccaacc tgcggctaac gtggcggcaa taccaaaccc gccccacaat
     3301 gcataagcga cagacaagtc gatcccttta acggcttgag aaagcgcact aaaggcagcc
     3361 agcaccgccg ccagggagag caagccaaat attttgcgac gaaagccgtc agaaaatttc
     3421 aaaaagacgt tagcaacgat ttccagcacg attgccaatg ccagccaggc ggcgtgaacc
     3481 cattcaaact gcgccatggt tcacctccag ttcaggttta cgcgctttac gggtacctga
     3541 ttttatcaac acaatcccgg cgaccagggt ggttaacccg gcaattttca tcagcgataa
     3601 actttcgtcg aataacaaaa cgctaaacaa ggtaataaat aaaataccga taccttccca
     3661 cagcgcataa gctacgccta aggcgatttt tttaacggcg aaagagagaa atatatacga
     3721 cagagaaatc atcaccagca ttaaaataaa gccgccattt ccctcactga cgctcgccca
     3781 tttcattgac agcgtaccgg taatttctgt agcaatagcc agacctaata aaatccaata
     3841 aatatacatt gtccctctcc tgcaagagaa ttattttaat tttcgcttaa ttcagcgaaa
     3901 ccaaagtaaa gtggtcaagc cccgcgcata gcgcaagctt tggcagaaga aaggactaaa
     3961 gcgcgttgcg ccagtgctgc tcacctacga gcaagatagt ggatgaggta cgaagatggg
     4021 taaatgtaga aaataacgtc ctgaacaaat tgtccataat attacaatta tccgcagtgt
     4081 tgcttctcgt catcgcggat gataattgtc ctcggtagtt gaacacgcct gatttgtatc
     4141 atagcttaag aattaactca aaatattttc acttctttac ctgagcggtt tgattttcgt
     4201 tatgatgacg gagcgaaaaa gacattatta ttagcaaagg aagaaaaaac ggggacaagc
     4261 atggcaaagc cgatcatcac gctcaatggc ctaaaaatcg tcattatgtt gggaatgctg
     4321 gtcattattc tctgcggtat ccgttttgcc gccgagatca tcgtgccgtt tattctcgca
     4381 ttatttattg ctgttattct taacccgctg gtgcaacaca tggtccgctg gcgtgtgccg
     4441 cgtgtactgg cggtgtcgat tttgatgacc atcatcgtga tggcgatggt gttgctatta
     4501 gcttatctgg gttccgcgct caacgagttg acgcggacgt taccgcaata tcgcaactct
     4561 attatgacgc cgctgcaagc gcttgaaccg ttgttgcaac gcgtagggat tgacgtctca
     4621 gttgaccagc tggcgcatta tattgatccg aacgcggcga tgacgttgct caccaactta
     4681 ttgacgcagt tatctaatgc catgtcatca atatttttat tgctgctgac ggtgctgttt
     4741 atgctgctcg aagtgccaca attgcccgga aaatttcagc aaatgatggc gcgtccggtt
     4801 gaagggatgg cggcgattca acgtgcgatt gacagtgttt ctcattatct ggtgctgaaa
     4861 acagccatca gcatcatcac cggcctggtc gcctgggcga tgctcgccgc actcgatgtt
     4921 cgcttcgctt ttgtctgggg attgctggcc tttgcgctta attacatccc gaatattggt
     4981 tcagtcctcg cggcaatccc ccctatcgct caggtactgg tgtttaatgg cttctacgaa
     5041 gcgttgctgg tgctggcggg atatctgctg attaatctgg tcttcggcaa tattctggag
     5101 ccgcgtatca tggggcgtgg gctggggctt tccacattgg tggtattttt gtcgttgatt
     5161 ttttggggat ggttgttagg accggtgggt atgctgcttt ccgtgccgtt gacaattatt
     5221 gtcaaaattg cgcttgaaca aacagcggga ggtcaaagca tcgccgttct gttaagcgat
     5281 ctcaataaag agtgacggcc tcagcagagg ccgtcagggt tacagagctt tcaggattgc
     5341 atccacgctg gctttggcgt caccaaacag catgtgggtg ttttccttga agaacagcgg
     5401 attttgcaca ccagcatagc cagtgttcat cgaacgttta aagacaatca cgttctgcgc
     5461 tttccacact tccagcacag gcataccagc aatcggactc ttcggatcat cctgcgccgc
     5521 cgggttaacc gtatcgttag caccaatcac cagtacggta tcggtatcag caaagtcatc
     5581 attgatctcg tccatttcca gcacgatgtc atacggtact tttgcttcag ccagcaatac
     5641 gttcatatgt ccaggcaaac gccccgcgac cgggtggata ccgaaacgca cattaatacc
     5701 acgagcgcgc aatttctcag taatttcagc gacaggatat tgcgcctgcg cgactgccat
     5761 gccgtacccc ggagtaatga tcactgaatg ggagtttttc agcagttccg ctgtctcttc
     5821 tgcggtgatt tcgcggtgct cacccacttc ctgatcatcg ccagtagaag agccgtcggt
     5881 gccgaaacca cccgcaataa cgctgataaa ggaacggttc atcgccttac acataatgta
     5941 agaaaggata gcccccgaag aaccgaccag cgcacccgtc acaatcagca ggtcgttgct
     6001 gagcataaag cccgcagccg cagccgccca gccggagtac gagttcagca tcgacaccac
     6061 cactggcata tctgcaccac cgatggaggc gactaaatgc cagccgaata ccagcgcaat
     6121 tgcggtcatt atcagcaatg ccagcacttg caggccgacg ctgtccgtgc gaacaaatac
     6181 aatcagcagc aggaaggaaa cgaccagagc cgccaggttc attttgtgac ggtttggcag
     6241 catcaatggt ttagacgaaa tcttgccaca cagtttgccg aacgccacca ccgaacccgt
     6301 gaacgttacc gccccgatga agataccgag gaacacttcc gtcaggtgaa tattgaccag
     6361 aatcggtgcc attcccgcgt catgatgcag atagctgtta aagccaacca gcactgccgc
     6421 cagacccacg aagctatgca ggatcgccac cagttctggc atttcggtca tttcaacttt
     6481 cttcgccaga cggataccaa ttgccccacc aatgaccatc gccagcaaga tccagccaac
     6541 attacccgta tccggtccaa aaatggttgc gattaacgca atcgccatcc cggcgatacc
     6601 gaagttgtta ccctggcgag acgtttcatg tttcgaaaga ccggccagac tgaagataaa
     6661 caggatcgcg gcaacaatgt atgcagctgt aactaatcct ccagacatat gttacccctt
     6721 aatttttgcg gaacattttc agcatgcgct gagtcacggt gaagccaccg aaaatattaa
     6781 tgctggctat aagcaccgcg ataaaactaa ggaagctaac ccagccgccc tggccaatct
     6841 gcaacagtgc tccgacaaca ataatccctg aaatcgcgtt ggtgaccgac atcaacggtg
     6901 tatgcagcgc gtgcgataca ttccacacca cgtaataacc gacaacgcag gccagcgcga
     6961 aaacggtgaa gtgcccaagg aattctttcg gcgcaacgct tgccatccag ccaaaaagaa
     7021 tgattgccag cgccatcaac gcgtatttac gccacggtga gcaggtacat ttttcctcag
     7081 ttttcacttc cggtgccgct ttttgtgccg cctgcggctg agctgatacc tgaatcggcg
     7141 gtgccggcca ggtaatttcg cccgcacgga tcacggtcac gccgcgaatc accacatcat
     7201 caaaatcaac agtgatattg ccgtctttct ctttgcacaa cagtttcagc agattaacga
     7261 ggtttgtgcc gtaaagctgt gaggattgcg tcggcagacg gcccggaaga tcggtataac
     7321 caatcacttt gacaccattt tccgtagtga agatttcacc cggcacggtg tattcacagt
     7381 tgccgccgtt ttgggctgcc aggtcgacaa tcacactgcc cgccttcatg gagtcaacca
     7441 tttcacgggt aattagcttc ggcgctggtt tgcctggaat aagcgcggtg gtgacaatga
     7501 tatcgacctc ttttgcctgg gcggcaaaga gttccatttc cgctttgatg aacgcgtccg
     7561 acatcacttt ggcatagcca tcgccgctgc cagcttcctc tttaaaatcc agctcgagga
     7621 attccgcgcc catactttga acttgttctt tcacttccgg gcgggtgtcg aatgcacgca
     7681 caatcgcgcc gagactgttt gctgcgccaa tggcggccag acctgcaaca cccgcaccaa
     7741 tcaccatcac ttttgccggt ggcactttcc cggccgcagt aatttgcccg gtaaagaagc
     7801 gcccaaattc atgtgccgct tcaacaatgg cgcgataacc ggcgatgttc gccatcgagc
     7861 ttagtgcgtc cagcgattgt gcgcgtgaga tacgcggcac agagtccatc gccatcacgg
     7921 tcacgttacg ttccgcaagt ttttgcatta attccggatt ctgcgcaggc cagataaaac
     7981 tcaccagcgt tgtcccagga ttcagtaacg caatttcatc atctaacggc gcattgacct
     8041 tcagaatgat ctctgactgc cagacgctat tcccttctac aatttcagcg cccgcttgca
     8101 caaacgcttt atcgtcaaaa cttgccagtt gacccgcgcc gctctctacc gcgacggtaa
     8161 aacccagttt cagcagctgt tccactgttt ttggcgttgc tgcaacacgg gtttcattgg
     8221 ttaaccgttc tcttggtatg ccaattcgca tgatattccc ttccatcggt tttattgatg
     8281 atggtttgcc tgtgtcagga gccacacaag ctgctcatgt acgagctaaa tgttactccg
     8341 ttaaaataaa ttagtaacaa acgccctata acgtactgaa aattatgcct gtgatctagc
     8401 gccaaaaatc agtatttcgg cgtgaactcg caaaatatta acgattcagc cgtgatagtg
     8461 ggataaacac cttagaacgc cggataaaga ctgataattg tcttcgacgg tcgggtaaaa
     8521 cgagacatcg ccccggcacg aatcactact taacattaaa ttaacttata caattcagtt
     8581 gcttcagtag taatgatgct gatacggctg ttttttaagc atagacggtc atttgagcag
     8641 gattaaaatt ggcttaagga atgtgatatg aaaaatgacg cagacagtta caccgtttaa
     8701 atgcaataat cagccacgtt tctcgttaat aacaatacca gtacctggtt tgcgcaaggc
     8761 gaaggattat ttttatgaag cttaagaaca ccctcctggc gtcggcactg ctttctgcta
     8821 cggcattctc cgttaacgca gcaacagaac tgacaccgga gcaagcggca gcggttaaac
     8881 cttttgaccg tgtagtggtt accggtcgtt ttaatgctat tggcgaagcg gtgaaagccg
     8941 tttctcgtcg cgcagataaa gaaggtgccg cctcttttta tgttgtcgac acttctgatt
     9001 ttggtaacag cggtaactgg cgtgtggtcg ctgacctcta taaagccgat gctgaaaaag
     9061 cagaagaaac aagtaatcgc gtaattaacg gtgttgtcga actgccgaaa gatcaggctg
     9121 ttctgattga accgtttgac acggtcaccg tccagggctt ctatcgtagc cagccagaag
     9181 tcaatgatgc catcaccaaa gcggcaaaag cgaaaggtgc ctactctttc tacatcgttc
     9241 gtcaaatcga tgccaaccag ggcggcaacc agcgtattac tgcattcatc tataaaaaag
     9301 atgctaagaa acgtatcgtc cagagcccgg atgtgatccc ggcagattcc gaagcaggac
     9361 gtgcagctct ggctgccggt ggcgaagccg cgaagaaagt tgagatcccg ggtgttgcga
     9421 ctaccgcatc accaagttct gaagtcggtc gcttctttga aacccagtca tcaaaaggcg
     9481 ggcgttacac cgtcacgctc ccggatggca ctaaagtcga agaactgaac aaagcgaccg
     9541 cagcgatgat ggtcccgttc gacagcatca aattctctgg caactacggc aacatgaccg
     9601 aagtctccta tcaggttgcg aaacgtgccg cgaagaaagg tgctaagtat taccacatca
     9661 cccgccagtg gcaggaacgt ggtaataacc tgaccgtcag cgcagatctg tataaataac
     9721 tattcagtta ctcctataag gcggcttgat gccgcctttc gcatttttag tcacaaattt
     9781 ccccgcgacc attgcatgca gacacatctc ttcgtaaaat cccgcgcttc agtggcaaac
     9841 tccattttta tgcgttttgc cctaatattt attcttctta tcacgtttta atcactggat
     9901 atcgatggaa aagaaactgg gactgagcgc actcaccgcg ctggtattaa gctcaatgct
     9961 gggcgcgggt gttttcagtc tgccgcaaaa tatggcggca gttgccagcc cggcagcact
    10021 gctcatcggc tggggtatta ctggcgctgg cattttattg ctggcctttg ccatgctgat
    10081 cctcacgcgc attcgtcccg aacttgacgg cggtatcttt acctatgccc gcgaagggtt
    10141 tggcgagcta atcggctttt gttccgcatg gggatactgg ctgtgcgcag tcatcgccaa
    10201 cgtctcctat ctggtgatcg ttttttccgc gttaagcttt tttacggaca cgccggaatt
    10261 gcgcctgttt ggcgatggta atacctggca atcgatagtc ggtgcatcgg ctttattgtg
    10321 gattgttcac tttttgatcc tgcgcggggt gcaaaccgct gccagcatta acctggtggc
    10381 gacactggca aaattgttgc cgctgggtct gtttgttgtg ctggcgatga tgatgttcaa
    10441 actggatacc ttcaagctcg acttcaccgg acttgcactt ggcgtacccg tttgggaaca
    10501 ggtgaaaaac accatgctga tcaccctgtg ggttttcatt ggtgtggaag gtgccgtggt
    10561 tgtttctgcg cgtgcgcgta ataaacgtga tgttggcaaa gcgacactgc tggcggttct
    10621 ctccgctctg ggcgtttact tgctggtaac gctgctttcg ctgggtgtgg tggcacgtcc
    10681 tgaactggct gaaattcgta acccgtcaat ggccggtctg atggtggaaa tgatgggacc
    10741 atggggcgaa atcatcatcg ctgccggttt gatcgtttcc gtttgcggcg cgtacttaag
    10801 ctggaccatc atggcagcag aagtaccgtt cctggccgcc actcataaag cattcccgcg
    10861 catttttgcg cgccagaacg cacaagctgc gccatccgcc tcactgtggt tgaccaacat
    10921 ctgcgtgcaa atttgtctgg tactcatctg gctgaccggt tccgattaca acacgttgct
    10981 gaccatcgcc tcagaaatga ttctggtgcc ctatttcctg gtcggcgcgt tcttgctgaa
    11041 aatcgcaacc cgtccactgc ataaagcggt aggtgtcggt gcctgcattt atggcttatg
    11101 gttattgtac gcctctggtc caatgcactt gctgttatcc gttgttctgt atgcaccggg
    11161 gctgctggtt ttcctgtacg cgcgcaaaac gcatacccat gataatgtac tgaaccgtca
    11221 ggaaatggtg ctgattggca tgctgttaat tgcctcagtt ccggcgacct ggatgctggt
    11281 gggataacgt tcatcccatc gttggatagc aaaggagata acgatgggta aaacccagcc
    11341 cttgccaata ttaattactg gcggaggtcg tcgcatcggc ctcgccctcg catggcattt
    11401 cattaatcaa aagcaaccgg tgattgtcag ctatcggaca cactatccag ccattgatgg
    11461 actgattaat gcaggtgcgc agtgtattca ggctgatttt tcgaccaacg acggtgtgat
    11521 ggcgtttgcc gatgaagtac taaaaagcac ccatggtctg cgtgctattt tgcataacgc
    11581 cagtgcgtgg atggcggaaa aaccgggtgc gccactggcc gacgtactgg cttgcatgat
    11641 gcagatccac gttaataccc catacctgct caaccatgcg ctggaaagat tactgcgtgg
    11701 gcacggacac gccgccagcg atatcattca ctttaccgat tatgtggtgg agcgcggtag
    11761 cgacaaacat attgcgtatg ctgcaagcaa agcggcactg gataatatga cccgctcgtt
    11821 tgcccgcaag ctggcaccgg aagtgaaagt gaattctatt gcgccatcgc tgatcctgtt
    11881 taatgaacat gatgatgccg aatatcgaca acaggcgctg aataaatcac tgatgaaaac
    11941 cgcgcctggc gagaaagaag tgatcgacct ggtcgattac ttacttacca gttgctttgt
    12001 caccggacgc agtttcccac ttgatggcgg tcgtcatctg cgttaatgca gctttatcca
    12061 gcatataatc aacacccatg cgcttatccc ccaacacgca acagaaccaa caaaagcggc
    12121 gggcaagcgc atcattccgg tgaaatacca cagcgacacc aggtaaacaa aatagggaat
    12181 aatcgaccac atactaaaaa tgatggttgc gcgtaaggct tcaatgccgc gttcgctggc
    12241 aacaatataa tgcgcgataa gcgcaaaggt cgggaaaagt ggaatcagcc cggcgatata
    12301 ataatttttc gtttttgcta aaacaccaat caacagcact accagcgcac cgagcgcggc
    12361 tttgattacc agccccatct ttttacctta acacttccat aacaagtcat cagtagaata
    12421 cctgatgaaa acttgtttag aaacgattga tagtaagtaa aaacagcgcg gtgtattgtg
    12481 acgtttttat atctaccgtg aatgttatga acactatcgt atttgtggaa gatgatgcgg
    12541 aagtcggttc actgattgcc gcgtacctgg caaaacatga tatgcaggtt accgtagagc
    12601 cgcgcggcga ccaggccgaa gaaaccattt tgcgagaaaa tccggatttg gtgttactcg
    12661 acatcatgct accaggcaag gacggcatga ccatttgtcg tgatttacgc gcaaagtggt
    12721 ctggaccgat tgttcttcta acctctctcg atagcgatat gaaccacatc ctggcactgg
    12781 aaatgggtgc ctgcgactat attctcaaaa cgacgccccc tgctgttttg ctagcgcgtt
    12841 tacgtttgca tttgcgtcag aatgagcaag ccacactgac caaaggtctt caggaaacgt
    12901 ctctgactcc ctacaaagcc ctgcatttcg gcacgttgac catcgatccc atcaaccgcg
    12961 tagtcaccct ggctaacact gaaatctcgc tctcgacagc tgatttcgaa ttattgtggg
    13021 aattagctac ccatgccggg caaatcatgg accgcgatgc attgctgaaa aatttacgcg
    13081 gcgtcagtta tgacggactg gatcgtagcg tggacgtggc tatttcgcgg ttaagaaaaa
    13141 aactactcga taacgccgca gaaccttatc gcattaaaac cgtgcgtaac aaaggctatc
    13201 tttttgcgcc tcatgcatgg gaataagcga tgaaaaaatt gtttatccag ttttacctgt
    13261 tattgtttgt ctgcttcctt gtgatgtctc tgctggttgg gctggtgtac aaatttaccg
    13321 ccgaacgcgc gggcaaacag tcgctggatg atttgatgaa cagttcgctg tatctgatgc
    13381 gcagcgaatt gcgtgagatc cccccacacg actggggtaa gacgctgaaa gagatggatt
    13441 taaatctctc tttcgatctg cgtgtcgagc cactgagtaa ataccatctt gatgatattt
    13501 ccatgcaccg actgcgtggc ggcgaaattg tcgccctgga cgatcagtac acgtttttgc
    13561 agcgtatccc gcgcagccac tacgtgctgg cagttggtcc tgttccttat ctttattacc
    13621 tccatcaaat gcgattgctg gatatcgccc tgatcgcttt tattgctatt tccctcgcct
    13681 ttccggtgtt tatctggatg cgtccgcact ggcaggatat gttaaaactg gaagcagcgg
    13741 cgcaacgatt tggcgatggg catctcaatg aacgtatcca ctttgatgag ggttcgagct
    13801 ttgaacgact tggcgtcgca tttaaccaga tggcggacaa tatcaacgcc ttaattgcca
    13861 gcaaaaaaca gcttattgac ggtatcgctc acgaactgcg aacaccgtta gtgcgcctgc
    13921 gttatcgact ggagatgagc gataacctga gcgccgccga atcccaggcg ttgaatcgtg
    13981 atatcagtca acttgaagct ttaattgaag agctgctgac ttatgcccga ctcgatcgcc
    14041 cacaaaacga gcttcatctt agcgaaccag acctgccgtt gtggctgtca acgcatctgg
    14101 cagatattca ggcagtaacg cccgataaaa cggtacggat aaaaacgctc atgcaaggcc
    14161 attatgcggc gttggatatg cgcttaatgg agcgcgtgct ggataatttg ctcaataacg
    14221 ccctgcgcta ctgccattca acggttgaaa ccagcctgct actgtcgggg aataaagcga
    14281 cattaattgt tgaggatgat ggctcaggga ttgccccaga aaaccgcgaa catatctttg
    14341 aaccttttgt tcgcctcgat cccagccggg gtcgctcaac cggcggctgc gggctgggac
    14401 tggcaattgt ccactctata gcactggcaa tgggcggtac ggttaattgt ggcaccagcg
    14461 aactgggtgg tgcccgcttc tcgtttagct ggccgttatg gcataacatc ccgcaattta
    14521 cctctgcttg acactacgcg cacggtggtc aagtcaccac cactgtgcta taaattaagg
    14581 atgttgtaac taaagtggtt aatattatgg cgtgttacga tctcgtagac cgactcaaca
    14641 ctacttttcg ccagatggaa caagagctgg ctgcatttgc cgctcatctt gagcaacaca
    14701 agctattggt tgcccgcgtg ttctctttgc cggaggtaaa aaaagaggat gagcataatc
    14761 cgcttaatcg tattgaggta aaacaacatc tcggcaacga cgcgcagtcg ctggcgttgc
    14821 gtcatttccg ccatttattt attcaacaac agtccgaaaa tcgcagcagc aaggccgctg
    14881 tccgtctgcc tggcgtgttg tgttaccagg tcgataacct ttcgcaagca gcgttggtca
    14941 gtcatattca gcacatcaat aaactcaaga ccacgttcga gcatatcgtc acggttgaat
    15001 cagaactccc caccgcggca cgttttgaat gggtgcatcg tcatttgccg gggctgatca
    15061 cccttaatgc ttaccgcacg ctcaccgttc tgcacgaccc cgccacttta cgttttggct
    15121 gggctaataa acatatcatt aaaaatttgc atcgtgatga agtcctggca cagctggaaa
    15181 aaagcctgaa atcaccacgc agtgtcgcac cgtggacgcg cgaggagtgg caaagaaaac
    15241 tggagcgaga gtatcaggat atcgctgccc tgccacagaa cgcgaagtta aaaatcaaac
    15301 gtccggtgaa ggtgcagccg attgcccgcg tctggtacaa aggagatcaa aaacaagtac
    15361 aacacgcttg ccctacaccg cttattgcgc tgattaatcg ggataatggc gcgggcgtgc
    15421 cggatgttgg ggagttgtta aattacgatg ccgacaatgt gcaacaccgt tataaaccac
    15481 aggcgcagcc gcttcgtttg atcattcccc ggctgcacct gtatgttgcg gattaacgcc
    15541 cggctttcat actgccgacc atctgttctg gccgtaccca gctgtcaaac tcggcttcgc
    15601 taagataccc cagcgcaagg gccgcagctt ttaaggtcag cccttcttta tgcgcttttt
    15661 tggcgatctc cgcggctttg tcataaccaa tgtgggtgtt aagcgcagcc accagcatca
    15721 gcgattcatt gagtaattga ttgattcgct cacgattcgg ttcaataccc actgcgcagt
    15781 gtttgttaaa actttccatg ccatctgcca gcaagcgcac cgattgcagg aaattgtgga
    15841 tcaccattgg acggaagacg ttcagttcaa agttaccgga agcgcccccc atgttgatcg
    15901 ccacgtcgtt ccccatcacc tgacagcaga gcatggttaa tgcctcacac tgtgttggat
    15961 tcactttccc cggcatgatt gagctgcccg gctcattttc cgggattgag atttcaccaa
    16021 ttccgcagcg cgggccagag gccagccagc ggacatcatt ggcgattttc atcagtgacg
    16081 cagccaaccc tttcaacgcg ccgtgcgcct gaaccagagc atcacaggtc gccagcgctt
    16141 caaatttgtt cggcgcggta acaaacggtg cacaggtaat gactgccagt tcatctgcta
    16201 cgcgacgcgc atactccgga tgggtattta gtccagtacc caccgctgta ccgccaagag
    16261 ccagttccgc tacgtgaggc aggctgtatt cgatatgttt gagattatac tcgagcatcg
    16321 ctacccagcc ggaaatctcc tgccccagtg ttagcggcgt ggcgtcctgc aagtgggttc
    16381 gaccgatttt gacgatatcg gcaaatgcac gcgatttttc actcagcgtc tgtgtcaggg
    16441 ttttaagctg cggaatgagt tgcttgcgca gcgccagcag cgccgcaacg tgcatcgccg
    16501 tcggaaagac atcgttggaa ctttggcttt tgttcacgtc gtcgttaggg tgaactttac
    16561 gttccatccc gcgcacgccg ccgagtaatt cactggcccg gttagccagc acttcgttaa
    16621 tgttcatgtt actttgcgtg ccggagccgg tctgccagat agccagcggg aattcgtcgt
    16681 catgctgtcc tgccagtact tcatccgccg cctgccgaat ggcgctcgct ttctcttcag
    16741 acaacaagcc taaatcttca ttaacttttg ccgctgcgcg cttggttagc gccagcgcat
    16801 gaatcagtga ggtgggcatt ttctccgtcg aaatgcggaa atgctccagc gagcgttgag
    16861 tttgtgcgcc ccacagctta tctgccggga catcaatcgc ccccatcgaa tctttttcgc
    16921 tgcgtactgt attcatggcc tgctcctcac ctgtttaatt ttttctttct gttttgcttt
    16981 cgttaagcaa cttttagctc acttattatt tactatttga taacaaatgt ttggtctttc
    17041 gtgccatgta aaaaaaccgc cccgaagggc ggctctgttt atttcacaca gcgagtgcac
    17101 tgtgtgagtt gtatctgctg gaagaagtca tttcctttat catccacaag gataaacgcc
    17161 gggaaatctt ccacttcaat tttccagatg gcttccattc ccagttccgg atattcaaca
    17221 cattccaggc tcttaatact tccctgcgcc aatacagcgg ccggaccacc gatactgcca
    17281 aggtagaagc cgccgtgttt tttacaggca tccgtcacct gctggctgcg gttgcctttc
    17341 gccagcatga tcatacttcc gccctgcgct tgcagttgat cgacataaga atccatccgt
    17401 ccggcggtcg ttgggccaag agaaccggag gcataacctt ccggcgtttt ggccggaccc
    17461 gcgtagtaaa tcggatgatc tttaatgtac tgcggcagcc cttcaccgtt atccatccgc
    17521 tctttcagtt tggcgtgagc aatatcacga ccgacgataa tcgtgccgtt aagcgataag
    17581 cgtgtagaaa cgggatactg cgacaactgt gcgaggatct ctttcatcgg acggttaagg
    17641 tcaacgcgca ccgcttcgcc ttctcccgct ttacgcagct cttcagggat atatttacct
    17701 ggattatgtt ccagtttttc gatccagatc ccctgacggt tgatcttcgc tttgatatta
    17761 cggtcagcag agcaggaaac acccatacca accggacagg atgcgccgtg acgtggcagg
    17821 cgaatcacgc gtatgtcgtg agcgaagtat ttaccaccaa actgcgcacc cagaccgaga
    17881 ttttgcgctt cgatcagcaa ttctttttcc agttccacat cgcggaatgc ctgaccgtgc
    17941 tcattccctt ccgttggcag ttcgtcatag tatttagcgg aagccagttt cactgtttta
    18001 aggttagttt ctgcagaagt tccaccaatt acgaacgcaa tatgatacgg aggacaggcc
    18061 gccgtaccca gcgtgcgcat cttctcaacc aggtaatttt tcagtttccc cggcgttagt
    18121 aacgctttgg tttcctgata gagatacgtc ttgtttgccg aaccaccacc tttggcgata
    18181 cagaggaatt tgtactcgtc gccatcaacg gcataaagat cgatctgcgc tggcagattg
    18241 gtgccggtat tcacctcttt atacatatcc agcggcgcgt tttgcgagta gcgcaaatta
    18301 tcttcgatat aagtgttata gacaccgcgc gccagcgccg cttcatcaca gccaccggtc
    18361 catacacgct gccctttttt accaacaata atcgcagtgc cggtatcctg acaggttggc
    18421 agaacgcctt tcgccgcgat gtcggagtta cgcaggaatt gcagcgccac atatttatca
    18481 ttttcgctgg cctccgggtc acgcagaatg tcggccacct gttgttggtg agccggacgc
    18541 agcataaacg acgcatcatg aaacgcctga cgcgccaaca gagttaacgc ttcgggtgcg
    18601 actttcaaaa tctcctgccc ttcaaattca gatacgctaa cgtgttcgct ggttagcagg
    18661 taatactcag tatcatcttt tttgagtgga aaaggagcct gataatgaaa gggtttgttt
    18721 gacattgttc tctcacttac tgcctggttt ggttatgctc tgggcgggtg ttccgttgtc
    18781 ctgttaaaag cgagtaacaa tatcctacac acttttttaa caaaaactga gactagtacg
    18841 actttttgcg gctccaggtt acttcccgta ggattcttgc tttaatagtg ggattaattt
    18901 ccacattaaa acagggattg atcatgcaaa aactcattaa ctcagtgcaa aactatgcct
    18961 ggggcagcaa aacggcgttg actgaacttt atggtatgga aaatccgtcc agccagccga
    19021 tggccgagct gtggatgggc gcacatccga aaagcagttc acgagtgcag aatgccgccg
    19081 gagatatcgt ttcactgcgt gatgtgattg agagtgataa atcgactctg ctcggagagg
    19141 ccgttgccaa acgctttggc gaactgcctt tcctgttcaa agtattatgc gcagcacagc
    19201 cgctctccat tcaggttcat ccaaacaaac gcaattctga aatcggtttt gccaaagaaa
    19261 atgccgcagg tatcccgatg gatgccgccg agcgtaacta taaagatcct aaccacaagc
    19321 cggagctggt ttttgcgctg acgcctttcc ttgcgatgaa cgcgtttcgt gagttttccg
    19381 agattgtctc cctactccag ccagtcgcag gtgcacatcc ggcaattgct cactttttac
    19441 aacagcctaa tgccgaacgt ttaagcgaac tgttcgccag cctgttgaat atgcagggtg
    19501 aagaaaaatc ccgcgcgctg gcgattttaa aatcggcgct cgacagtcag cagggtgaac
    19561 cgtggcaaac gattcgttta atttctgaat tttacccgga agacagcggc ctgttctccc
    19621 cgctgttact gaatgtggtg aaattgaatc ctggcgaagc gatgttcctg ttcgctgaaa
    19681 caccgcacgc ttacctgcaa ggcgtggcgc tggaagtgat ggcgaactcc gataacgtgc
    19741 tgcgtgcggg tctgacgcct aagtacattg atattccgga actggttgcc aatgtgaaat
    19801 tcgaagccaa accggctaac cagttgttga cccagccggt gaaacaaggt gcagaactgg
    19861 acttcccgat tccagtggat gattttgcct tctcgctgca tgaccttagt gataaagaaa
    19921 ccaccattag ccagcagagt gccgccattt tgttctgcgt cgaaggcgat gcaacgttgt
    19981 ggaaaggttc tcagcagtta cagcttaaac cgggtgaatc agcgtttatt gccgccaacg
    20041 aatcaccggt gactgtcaaa ggccacggcc gtttagcgcg tgtttacaac aagctgtaag
    20101 agcttactga aaaaattaac atctcttgct aagcttatta aaggcttata acaccttcag
    20161 gcggccagtc cgcctgattt cattttatgg ataatcatta tgaataaatc gctggtagcg
    20221 gtaggcgtca ttgttgcgct aggcgtagtc tggacaggcg gcgcatggta tacaggcaag
    20281 aagattgaaa cccatctcga agacatggtc gcgcaggcga acgcgcaact caaactgaca
    20341 gctcctgaat ccaacctgga agtgagttat caaaactatc atcgcggcgt attcagcagc
    20401 cagttgcaac tgttggtgaa acccattgcc gggaaagaaa atccgtggat taaaagcggt
    20461 cagagcgtca tcttcaacga atcggttgat catggtccct tcccgcttgc ccagcttaaa
    20521 aaactgaacc tgatcccgtc gatggcatca attcaaacca cgctggttaa taacgaagta
    20581 agcaaaccac tgtttgatat ggcaaaaggt gaaacgcctt ttgagattaa ctcgcgcatt
    20641 ggttacagcg gtgattccag ttccgatatt tcgctcaagc cactgaatta cgagcaaaag
    20701 gatgaaaaag tcgcctttag cggcggcgag ttccagttaa atgctgacag agacggcaaa
    20761 gccatctccc tttccgggga ggcgcaaagt ggtcggatag acgcagttaa cgaatacaac
    20821 cagaaagtgc agttgacctt taataatctg aaaaccgacg gttccagcac gctggcaagt
    20881 tttggtgagc gcgtaggaaa ccaaaaactg tcactggaaa aaatgaccat ttcagtggaa
    20941 ggcaaagaac tggcactgct ggaaggcatg gagatcagcg gtaaatcgga tctggtcaat
    21001 gacggtaaaa cgatcaatag ccaactggat tactcgctaa acagcctgaa ggtacagaat
    21061 caggatctgg gcagcggcaa gctgacttta aaagtcggcc agattgatgg tgaagcctgg
    21121 catcagttta gccagcaata taacgcgcaa actcaggcgc tgctggcaca gccagaaatt
    21181 gccaacaatc ccgaacttta tcaggagaaa gtgacggaag ccttctttag cgccctgccg
    21241 ctgatgttga aaggcgatcc ggtgattact atcgcgccgc taagctggaa aaacagtcag
    21301 ggtgaaagtg cgctgaatct gtcgctgttc ctgaaagatc cggcaacgac taaagaagcg
    21361 ccgcaaacgc tggcgcagga agtagatcgt tcggttaaat ctctggatgc gaaactgacc
    21421 attccggtgg atatggcaac tgagtttatg actcaggtag cgaagctgga aggttatcag
    21481 gaagatcaag cgaaaaaact ggcgaaacag caagttgaag gtgcatcagc aatggggcag
    21541 atgttccgtc tgaccacctt gcaggacaat accatcacca ccagcctgca atatactaac
    21601 ggtcagataa cgttaaacgg gcagaaaatg ccactggaag atttcgttgg tatgtttgca
    21661 atgccggcat taaatgttcc ggtcgtaccc gctattccgc agcagtaatt cacagacaaa
    21721 acgccggaga tttttctctc cggcgttatt ttttacttca gcataaagtc atactgaata
    21781 ctcattttca catcccagta attcaggcga tcgcccagcg tactgccatc gcgcttttcc
    21841 cgccctttat cggcagcaag aaagaaattc atcccttgta acggcccttc gctgacccga
    21901 tagttcatac cacctaagat acccgtgcct tcaatccgca catctcgttt actggattca
    21961 tagccgcgag tcacaatcgg tgcaaatgtc agcgtaaatt gcggcgttaa acgatagttg
    22021 acgcccaatt gccaggactg cattccttca cggttacgat caagacttaa tgaaaaggga
    22081 tagccaatgt cggtatccac cacggcacca ttcattgcat ggcgcaaata accaccactg
    22141 ccaaatacac ttccccacgg cgcattccag gtcaattgtc cactaaccaa cgccgtttca
    22201 ttgggctggc tggtattacg acttaaacct tcgagctgcg cgtaaaagcc gagcaactcc
    22261 cctttcaacg tataattcgg gtcgagcggt atgcttttct cgagaatact ttgtatctga
    22321 ttggaaacat ctttctgtac cgatgccccc agagaatagc gactggtatc atcatgccaa
    22381 ctggcggcta aaaaagaacg tgcgcgatgt ttatcgtatt ttcccgtctg gctgttatac
    22441 accccttcat cacgaatacc ggttgttgaa tcagtccata aaaatactcg tgaacgccac
    22501 attccccaca gatcaatatt tggtgttaaa gcccaataac ctaatgctcc ttcagaaact
    22561 gtcggtaatg tacgttgcga actgccagcc attaacggaa tacgataagg taagctacca
    22621 taactataat ccgtcccgaa gcgacctaat ttaagcaggc tattttcact gggttttatt
    22681 tttactgccc ccagggcaaa gccaaaactt ttatcaccat caagatacca ccgggtactc
    22741 atatcagcac gagcacctaa tttataaaca taatacgccc cgccttcaac gccaataata
    22801 ttggaataat aaccactatt gaaatcgagc aatcctccct gtacccatgc gtaaatatct
    22861 cttcccgcgc cagcactggg cttatccgcc ctgcgcaatt cgtttttcag tctgatgcgt
    22921 agtcccgctt catcgtcagc cagttgcgcc gcataagcag aggtaaggcc agaggcagcc
    22981 gtcaggcaaa taacggccat ggccactatt tttctcatag ttgcaccttt gatgttctga
    23041 ttatttttat tgaatattaa ttagtgatat cgctgattaa ttgctgctgc acttttttac
    23101 gattatcaat ttcaaccacg atttctttga attttttatc cgtgagcgga taaaaccaga
    23161 taataacgaa tgccagtagc ataaatccgc aaggtactaa ggcaattgat gtgcggatgc
    23221 ccataataac ttccggcgtt tgcacctgat tggcgatata tccgcttaac cccaaaataa
    23281 aggcaggaat tgaacctccg attgcctgac cgcatttacg ggtaaatgag aatagtgaat
    23341 aggtgagccc ttcaattcgc acgccggtca ggtattcacc gtattctacg gtatcagctt
    23401 ccagcgccca catcacggtc atggtaacgc cctgaccaat tgaagcgatg gccaacgcaa
    23461 ccaacgccac cggcagtgac cagacggaaa cccagaagaa cagcagataa ccgcaggttc
    23521 ccagcaaagc gccaatcagg aaggtattct ttttaccgat cctcgcgacc atccccggca
    23581 ccagcggtgc cgatgccaca gtaccaacca ggttttgcac cagtaccagc acagtgaaca
    23641 gcccggtatc atttaacaca tagcgcacgt agaacaacga cgaggcgctg accgcaaagg
    23701 tcgaaatcag cacacacagc gcaccgatgc acaacataaa cagcgggcga ttccgtttca
    23761 gggtttgcag actgatattc aatgacggct gcgcaacgat acgtaccaca ttctcacgcg
    23821 tcgatttgaa gcagatgaag taaagcacca ttccggcaat cgccagcaca attgtccaga
    23881 aatggtatac cgacaccatc tcttccgggc tggagttctt aatgctcggt cctatcagaa
    23941 atgccaggca gacaaaggtc aatgaagcgg caatcccacg agccgcgccc agacgggcgc
    24001 gggattgtgg ttgttgggtc atcgcggtag caagtgaacc ataaggaata ttcaccaggc
    24061 tgtagcaaag cccgaggccc atgtaggtca aatatgcata caccactttg ctaccatggc
    24121 tccagtcggt cggcacccag aataccagca cgctgaagat cattaacggc gcagtaccga
    24181 agagtaaaaa cgggcggaat tttccccagc gggtattcac actgtccacc actcgtccgg
    24241 caaagacgtc ggcgaaggca tcgaataccc gcaccagtaa cagcatggtg cccgccgcag
    24301 cggcaccgac gccagcgacg tcggtgtagt aactcaacag gaagagcgcc cccattgcga
    24361 aggcgaagtt attggcgacg tcaccgaggc tgtagccgac gatggtgcgc caggagagtt
    24421 gttgattcat tgtttgcctc cctgctgcgg tttttcaccg aagttcatgc cagtccagcg
    24481 tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg cgagtgaaga tccctttctt
    24541 gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa tcggcgaaat tccatacctg
    24601 ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga tacatatcca gccatgcaca
    24661 ctgatactct tcactccaca tgtcggtgta cattgagtgc agcccggcta acgtatccac
    24721 gccgtattcg gtgatgataa tcggctgatg cagtttctcc tgccaggcca gaagttcttt
    24781 ttccagtacc ttctctgccg tttccaaatc gccgctttgg acataccatc cgtaataacg
    24841 gttcaggcac agcacatcaa agagatcgct gatggtatcg gtgtgagcgt cgcagaacat
    24901 tacattgacg caggtgatcg gacgcgtcgg gtcgagttta cgcgttgctt ccgccagtgg
    24961 cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt tcgttggcaa tactccacat
    25021 caccacgctt gggtggtttt tgtcacgcgc tatcagctct ttaatcgcct gtaagtgcgc
    25081 ttgctgagtt tccccgttga ctgcctcttc gctgtacagt tctttcggct tgttgcccgc
    25141 ttcgaaacca atgcctaaag agaggttaaa gccgacagca gcagtttcat caatcaccac
    25201 gatgccatgt tcatctgccc agtcgagcat ctcttcagcg taagggtaat gcgaggtacg
    25261 gtaggagttg gccccaatcc agtccattaa tgcgtggtcg tgcaccatca gcacgttatc
    25321 gaatcctttg ccacgcaagt ccgcatcttc atgacgacca aagccagtaa agtagaacgg
    25381 tttgtggtta atcaggaact gttcgccctt cactgccact gaccggatgc cgacgcgaag
    25441 cgggtagata tcacactctg tctggctttt ggctgtgacg cacagttcat agagataacc
    25501 ttcacccggt tgccagaggt gcggattcac cacttgcaaa gtcccgctag tgccttgtcc
    25561 agttgcaacc acctgttgat ccgcatcacg cagttcaacg ctgacatcac cattggccac
    25621 cacctgccag tcaacagacg cgtggttaca gtcttgcgcg acatgcgtca ccacggtgat
    25681 atcgtccacc caggtgttcg gcgtggtgta gagcattacg ctgcgatgga tcccggcata
    25741 gttaaagaaa tcatggaagt aagactgctt tttcttgccg ttttcgtcgg taatcaccat
    25801 tcccggcggg atagtctgcc agttcagttc gttgttcaca caaacggtga tacgtacact
    25861 tttcccggca ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg
    25921 ctccatcact tcctgattat tgacccacac tttgccgtaa tgagtgaccg catcgaaacg
    25981 cagcacgata cgctggcctg cccaaccttt cggtataaag acttcgcgct gataccagac
    26041 gttgcccgca taattacgaa tatctgcatc ggcgaactga tcgttaaaac tgcctggcac
    26101 agcaattgcc cggctttctt gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt
    26161 ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt ttgatttcac gggttggggt
    26221 ttctacagga cgtaacataa gggactcctc attaagataa taatactggt caacctttaa
    26281 tctgattagt taaccaattc tgtgttgcac acagttatct gtgagaaacc catggaagag
    26341 aaatggaaat aaaaagaact gacaccgcga tcaaaaaacc aaaaactgca atacccacag
    26401 cgtgatatag atcgcattaa tctttaaaac agcgttgaca gaagccataa aataagcaac
    26461 cgaacgttcg ttcgagcgta ttgggaacga taaactctca tgacatatgg ctacagtgaa
    26521 tattttgggg ggaattctcc gaagccggga aggataattt ttgcacagag aaattacgat
    26581 atgaaacgcg ggcacagtta tcaccagccc gccgaaaaag agagagaatc aggatgcggt
    26641 taagataccg ccaatcatcg cgcgtaatcc ctgagcaaac gacgcttcac gcgccaggtc
    26701 ttccgcttcg atatcggcca gtacaccgta ggtcagatcc agcagtaaac gtgaagtcat
    26761 tgccgtgttg atgtctgggc ttatctcgcc tttttgctgg gcatcacgca tccgctgggc
    26821 aacaaattcc gtgatcgtca tatgcttatt tttaagcatg gcggcgacct gtgggttacg
    26881 catcccttcc gccataattt caaccaccag cgcccgttgt ccaaaggctt catgggtgag
    26941 agagacaatg gactcgacca tatagtcaac gaaatgaatc ccttcaatcg gttcccggaa
    27001 acgggccagc gccctctcct ggtcctgtaa gataatcgcc tgaatcaagg cttctttgga
    27061 gatgaaatgg tgatagagcg tcccgggact aatggcgcaa gatttacaga tggctttcat
    27121 cgaggcactg tgaaatccat tttctgaaaa aatctctctg gcagcattga ggatccgggt
    27181 ccgtgtcggt tgtgcttcag tctgcatgtt atccatcatt aaaccattat agttagcgtg
    27241 aataactagt ttaccgcgtt gtcaggtgag cgcctatcat tgctgttgaa tagtgcgaag
    27301 gcacactcta ttcgtatact tttcactctg aaattggtta accaatagcc gtgatttggc
    27361 tgccagttat ttagcagaga cgttgatgag tttatcccgg taaacaacac gcacggttgt
    27421 ttaccggtcc gttagtgtat taattgagct cctgtacccc accaccggag acggtgagaa
    27481 tttgtccgct tacccagctc gcagcaggcg agcaaaggaa cagcgctgcg ttagcaatat
    27541 cttgcggttg gcccagacgt ctgatcggcg tgtgctgtaa cattttttgt tcaatttctg
    27601 gtgtaataac ggatttcagg gcatcggtta atattgcccc cggcgcaatg ccatttaccc
    27661 gaatattttt ttcacccagg tcaaacgcca tatttctgac cagatgactg gccgcagctt
    27721 tagatgatgc ataggaagtc atgtttatat ttttattttc tgccgccata gaagtgatgg
    27781 tcagaataac gccaccgcca tttttttcca tttctggcgc aacaagttgt gacagatgga
    27841 aaaaagaaaa cacattcagt tcataagcac ggcgaaaatc cgccattggc atatcaaacg
    27901 gtttaggtcc accgccaccg gcgttgttaa ccagaatatc aactttaccc agcttactga
    27961 tagcaaagtc tgccagtgca gagagttcct gttcggaagt aatatcacaa cggcaggcaa
    28021 atgcctgacc acccagttgt tgaatttcgt ctacaacatg gttagctgcg tcggcgttaa
    28081 tatcactgac caccacagat gcgccagctg tcgcgaatgt aatggcgatt tctttaccaa
    28141 tacctgcacc cgcacctgtg atgatggcgc attttccgtc gagtctcagg ttgtcagaat
    28201 taaacacata aacctcctgt gaacttcagt aaaaataaaa ggcaccgcct gtgtgccaca
    28261 ttttttatca ttaaccttga ggaaaggaca ctaccaggta tagcagataa agagaggatt
    28321 atttcgctgc aatgagccgg gcgggaataa taagattgcg tgaatgcgtc tcttcatggg
    28381 tgattttttg catcatgcga tccgcaagtg tgataccaag ttcccgcgct ggcgtgctgg
    28441 cccaggtaac aggtatatca tcaagtgtgg ttggtgtcgc atcggtaaat gccgccagcg
    28501 aaacctgttg ctcaaagtaa cgatcgactc cgctttcccc gctttgccgc cctgctttga
    28561 gcaaaccaaa ccatgccccc atcgcaatag tttcgttata gcaaaccacg gcactgatgg
    28621 tcgggttatg acgtaaaagc gccgtgatag cttccgcggc ttgcttctgg ctggaagtgc
    28681 actccaacac ccaatcgcta tgaaacggca ggccatattt tagtagagtt gcacaatagc
    28741 ccccaacccg ttctgcacgg gttaatgagg aactttgccc tcccagccag gcgatccgct
    28801 gatgcccatt gcgaatgaga tgctccgtca acaactgtgc agcctgcatg ttgtccgggc
    28861 gaaccgtatc aacatcatca agataactgg cacgggaagc gaaaatcacc gggatagctt
    28921 tttcttctgc catccgtcgc aggtcatcgc tacttcctgc agctccggca attaccacac
    28981 catcgacacc ctgattcagt aacagtgaaa accgctgtgc cagctgctca ccgtctttac
    29041 cgccgtgaag caaaaaaacc atccgtccct gcgcttccag agcttccgtc aatccggccg
    29101 tcaattcggc gtaaaacggc gcagataaat cacggacgat caaaccaatg acgccgcttt
    29161 gcccgccgcg cagcgccgac gcctggcgat tgcgcacaaa tcccagctct tcaatggcgg
    29221 cgttaacgcg ttctcctgtg gcggtagaga ttcgcccttt gccactaagc accagcgaaa
    29281 cggtacttac cgacacgccc gcagccagcg caacatcatg aatggttatt tttttggcgg
    29341 tagccatgca gatgacctac tccctgatta tgtgacagat aaaacgtttt accttttatt
    29401 tatcttatac ccgctattat cgttgcgtaa tgtgatttat gcctcactaa aatttgataa
    29461 aacgttttat cttctcgcgc aatttactga atccagattg ttctctacga ggagtcgttt
    29521 tatgacggcg aaaacagcac cgaaagtcac gctgtgggag ttcttccagc agttaggcaa
    29581 aaccttcatg ttacccgtgg cattattgtc gttctgcggc attatgctcg gcattggtag
    29641 ttctcttagc agccatgatg tcataaccct gatcccggtc ctgggcaacc ccgtgttgca
    29701 ggctatcttt acctggatga gtaagattgg ctcgtttgct tttagtttcc tgcctgtcat
    29761 gttctgtatc gccatcccgc tgggcctggc acgcgaaaat aaaggcgtag cggcattcgc
    29821 tggcttcgtc ggttatgcgg taatgaacct cgcggtaaac ttctggttga ccaataaagg
    29881 cattctgcca accacggatg ccgcggttct gaaagccaat aacatccaga gcattcttgg
    29941 gatccagtcg atcgataccg ggatcctcgg tgcggtgatc gccggtatta tcgtctggat
    30001 gctgcatgag cgtttccata atatccgcct gccggatgcg ctggcattct tcggcggtac
    30061 gcgcttcgta ccaattatct cctcgctggt gatgggcctt gtcggcctgg tgattccatt
    30121 agtctggccg attttcgcca tgggtattag cggcttgggc catatgataa acagcgcggg
    30181 tgatttcgga ccgatgctgt ttggtaccgg tgaacgtctg ctgttgccgt ttggtctgca
    30241 tcacattctg gtggcattaa ttcgctttac cgacgcaggc ggcacgcagg aagtctgcgg
    30301 tcaaaccgtc agcggcgcac tgaccatctt ccaggcgcaa ttgagttgcc cgaccactca
    30361 cggtttttct gaaagcgcca cgcgtttcct ttcgcaaggt aaaatgcctg cgtttctcgg
    30421 cggtctgcca ggtgcagcgt tagctatgta tcactgcgcg cgcccggaaa atcgccataa
    30481 aattaaaggt ctgctgattt ctggcctgat cgcctgcgtc gttggcggca ctaccgaacc
    30541 gctggaattc ctgttcctgt tcgtagcgcc agttctgtat gtcatccacg cgctgttaac
    30601 cggcctcggc ttcaccgtca tgtctgtgct cggcgtcacc atcggtaata ccgacggcaa
    30661 tatcatcgac ttcgtggtgt tcggtatttt gcatggtctg tcaaccaagt ggtacatggt
    30721 gccagtggtg gcggcaatct ggtttgtcgt ttactacgtc atcttccgtt tcgctatcac
    30781 ccgcttcaat ctgaaaaccc cggggcgcga tagcgaagtt gccagctcaa tcgaaaaagc
    30841 cgttgccggt gcgccgggta aatcaggtta caacgttcct gcaatcctcg aagcattagg
    30901 cggtgccgac aatattgtca gcctcgataa ctgcattacc cgtctgcgtt tgtctgtgaa
    30961 agatatgtcg cttgttaatg tgcaggcact gaaggacaat cgggcaattg gcgtagtaca
    31021 acttaatcaa cataacctgc aggttgttat cgggccacaa gttcagtcag taaaagatga
    31081 aatggccggt ctgatgcata ctgtccaggc ataaggataa gatatgttcg atttttcaaa
    31141 ggtcgtggat cgtcatggca catggtgtac acagtgggat tatgtcgctg accgtttcgg
    31201 cactgctgac ctgttaccgt tcacgatttc agacatggat tttgccactg ccccctgcat
    31261 tatcgaggcg ctgaatcagc gcctgatgca cggcgtattt ggctacagcc gctggaaaaa
    31321 cgatgagttt ctcgcggcta ttgcccactg gttttccacc cagcattaca ccgccatcga
    31381 ttctcagacg gtggtgtatg gcccttctgt catctatatg gtttcagaac tgattcgtca
    31441 gtggtctgaa acaggtgaag gcgtggtgat ccacacaccc gcctatgacg cattttacaa
    31501 ggccattgaa ggtaaccagc gcacagtaat gcccgttgct ttagagaagc aggctgatgg
    31561 ttggttttgc gatatgggca agttggaagc cgtgttggcg aaaccagaat gtaaaattat
    31621 gctcctgtgt agcccacaga atcctaccgg gaaagtgtgg acgtgcgatg agctggagat
    31681 catggctgac ctgtgcgagc gtcatggtgt gcgggttatt tccgatgaaa tccatatgga
    31741 tatggtttgg ggcgagcagc cgcatattcc ctggagtaat gtggctcgcg gagactgggc
    31801 gttgctaacg tcgggctcga aaagtttcaa tattcccgcc ctgaccggtg cttacgggat
    31861 tatagaaaat agcagtagcc gcgatgccta tttatcggca ctgaaaggcc gtgatgggct
    31921 ttcttcccct tcggtactgg cgttaactgc ccatatcgcc gcctatcagc aaggcgcgcc
    31981 gtggctggat gccttacgca tctatctgaa agataacctg acgtatatcg cagataaaat
    32041 gaacgccgcg tttcctgaac tcaactggca gatcccacaa tccacttatc tggcatggct
    32101 tgatttacgt ccgttgaata ttgacaacaa cgcgttgcaa aaagcactta tcgaacaaga
    32161 aaaagtcgcg atcatgccgg ggtataccta cggtgaagaa ggtcgtggtt ttgtccgtct
    32221 caatgccggc tgcccacgtt cgaaactgga aaaaggtgtg gctggattaa ttaacgccat
    32281 ccgcgctgtt cgttaacccc aattgcgcaa cgtaaaaaat cgttgcgcaa tcgtggattt
    32341 ttaccctgct ttgtttttat aatggtgcgc acttttatat ccagaaaaag agtgcgacca
    32401 tgattgatac caccctgcca ttaactgata tccatcgcca ccttgatggc aacattcgtc
    32461 cccagaccat tcttgaactt ggccgccagt ataatatctc gcttcctgca caatccctgg
    32521 aaacactgat tccccacgtt caggtcattg ccaacgaacc cgatctggtg agctttctga
    32581 ccaaacttga ctggggcgtt aaagttctcg cctctcttga tgcctgtcgc cgcgtggcat
    32641 ttgaaaacat tgaagatgca gcccgtcacg gcctgcacta tgtcgagctg cgtttttcac
    32701 caggctacat ggcaatggca catcagctgc ctgtagcggg tgttgtcgaa gcggtgatcg
    32761 atggcgtacg tgaaggttgc cgcacctttg gtgtgcaggc gaagcttatc ggcattatga
    32821 gccggacctt cggcgaagcc gcctgtcagc aagagctgga ggccttttta gcccaccgtg
    32881 accagattac cgcacttgat ttagccggtg atgaacttgg tttcccggga agtctgttcc
    32941 tttctcactt caaccgcgcg cgtgatgcgg gctggcatat taccgtccat gcaggcgaag
    33001 ctgccgggcc ggaaagcatc tggcaggcga ttcgtgaact gggtgcggag cgtattggac
    33061 atggcgtaaa agccattgaa gatcgggcgc tgatggattt tctcgccgag caacaaattg
    33121 gtattgaatc ctgtctgacc tccaatattc agaccagcac cgtagcagag ctggctgcac
    33181 atccgctgaa aacgttcctt gagcatggca ttcgtgccag cattaacact gacgatcccg
    33241 gcgtacaggg agtggatatc attcacgaat ataccgttgc cgcgccagct gctgggttat
    33301 cccgcgagca aatccgccag gcacagatta atggtctgga aatggctttc ctcagcgctg
    33361 aggaaaaacg cgcactgcga gaaaaagtcg ccgcgaagta acaaaatgga tggtgcaaat
    33421 gcaccatcca tttttcatgc aaggcacaaa gtcgcgcgat gtttggcgga ttcgatgccc
    33481 agctcaatca actccattac ctggattgcc tggcttgccg gaaccggatt ttcaccatcg
    33541 ccatttaacg catcacgaat agccgcatag taagccggat aattcccagg caccgtcaac
    33601 agcgtttctt cgacacgttc ctcaccttcc acgcgggtaa gtacgccatc acgcatatcg
    33661 tagccccagt cttcctgcgg tagacgctcg ccatttttca gacgttcttc ctgtggatcg
    33721 aggccatatt tcacataact gcctcgggat ccatgcacga tataccgtgc tgactcagcg
    33781 gctgccagca tggtaccgtg taaaatgact cgccgctgtg gataggacaa gatggcgtgg
    33841 aaataatcgg tcgactgcgc tccgggccgt aactgtgcca aatctaccgt catgctgacc
    33901 ggtaaaccaa aaagcgtaat ggcctgatca agaagatgtg gtgctaaatc gtaccagata
    33961 ccgctgcctg ggccgccctg ttcacgccaa cgatcgcgca cctgcggacg gaagcggtca
    34021 aaatgagact caaagtaagc aacttcaccc agcacgcctt ccgcgagtaa accttttagc
    34081 gtcaagaaat cgctatccca gcgacggtta tggaatacag acagcacacg ccccaggctt
    34141 tttgccagcg catccagctc tcgcgcttgt gacagtgtca cggtaaaggg tttatcaacg
    34201 accacatgtt tacccgcctc aagcgccgct ttggctaacg ggaaatgggt atcgttgggt
    34261 gtaggaatga caatcaggtc tatgttggga tcgttaaaca gatgcttcgg ctcagagaca
    34321 accgtaaccg ttggccagtc ggcttttact tttgtttcat cactgctgga gattaccgcc
    34381 agttccagcc cgggcgtgcc cgcaatcagg ggcgcatgga aggttttgct cgcataacca
    34441 tacccaatca acccaacacg gatgttgtcg ctcatgtcat tgcctctcat tgaagtatga
    34501 tggctatttg acactatcct ttacccacgc tcaacagttt aataacctgc cagcaataag
    34561 ggatgttgtt taacttaagt caaaaaaata gcgaattttc caacgacaaa agctaaatat
    34621 cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg ctaagtaaca tttaccccct
    34681 gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg aatcgtctta ttgaattaac
    34741 aggttggatc gttcttgtcg tttcagtcat tcttcttggc gtggcgagtc acattgacaa
    34801 ctatcagcca cctgaacaga gtgcttcggt acaacacaag taagctctgc acttgtggag
    34861 cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg atattaaacc aggaatttat
    34921 tatcttgttc gatgttgttg gtgattgtca gggatagtaa agcgcgttac tcttttcaga
    34981 gcagtttttc atccctgaat atttgccatc agcgcattct tattattgta ataacacaag
    35041 cgttcacttt tggtgaaa
//
LOCUS       DAJOSY010000037        34441 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJOSY010000037 DAJOSY010000000
VERSION     DAJOSY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.037"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            260..421
                     /locus_tag="OHN27_001922"
     CDS             260..421
                     /locus_tag="OHN27_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286370.1"
                     /translation="MEISFSPKRLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(505..1740)
                     /locus_tag="OHN27_001923"
     CDS             complement(505..1740)
                     /locus_tag="OHN27_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCQ2286371.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAEHHFLVGLSLDGPPEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAAREGLKLH
                     APGDIQGELTEWSVRPEEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(1887..1963)
                     /locus_tag="OHN27_001924"
     tRNA            complement(1887..1963)
                     /locus_tag="OHN27_001924"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1927..1929),aa:Pro,seq:tgg)
     gene            complement(2006..2092)
                     /locus_tag="OHN27_001925"
     tRNA            complement(2006..2092)
                     /locus_tag="OHN27_001925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2056..2058),aa:Leu,seq:cag)
     gene            complement(2113..2188)
                     /locus_tag="OHN27_001926"
     tRNA            complement(2113..2188)
                     /locus_tag="OHN27_001926"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2153..2155),aa:His,seq:gtg)
     gene            complement(2247..2323)
                     /locus_tag="OHN27_001927"
     tRNA            complement(2247..2323)
                     /locus_tag="OHN27_001927"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2287..2289),aa:Arg,seq:ccg)
     gene            complement(2426..3811)
                     /locus_tag="OHN27_001928"
     CDS             complement(2426..3811)
                     /locus_tag="OHN27_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2286372.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(4002..4742)
                     /gene="rffM"
                     /locus_tag="OHN27_001929"
     CDS             complement(4002..4742)
                     /gene="rffM"
                     /locus_tag="OHN27_001929"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCQ2286373.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4745..6097)
                     /gene="wzyE"
                     /locus_tag="OHN27_001930"
     CDS             complement(4745..6097)
                     /gene="wzyE"
                     /locus_tag="OHN27_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCQ2286374.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6094..7173)
                     /gene="rffT"
                     /locus_tag="OHN27_001931"
                     /gene_synonym="wecF"
     CDS             complement(6094..7173)
                     /gene="rffT"
                     /locus_tag="OHN27_001931"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCQ2286375.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7170..8420)
                     /gene="wzxE"
                     /locus_tag="OHN27_001932"
     CDS             complement(7170..8420)
                     /gene="wzxE"
                     /locus_tag="OHN27_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCQ2286376.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8422..9552)
                     /gene="rffA"
                     /locus_tag="OHN27_001933"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8422..9552)
                     /gene="rffA"
                     /locus_tag="OHN27_001933"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCQ2286377.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9557..10231)
                     /gene="rffC"
                     /locus_tag="OHN27_001934"
                     /gene_synonym="wecD"
     CDS             complement(9557..10231)
                     /gene="rffC"
                     /locus_tag="OHN27_001934"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCQ2286378.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFTLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10209..11090)
                     /gene="rfbA"
                     /locus_tag="OHN27_001935"
     CDS             complement(10209..11090)
                     /gene="rfbA"
                     /locus_tag="OHN27_001935"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCQ2286379.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11109..12176)
                     /gene="rffG"
                     /locus_tag="OHN27_001936"
     CDS             complement(11109..12176)
                     /gene="rffG"
                     /locus_tag="OHN27_001936"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCQ2286380.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYSDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12176..13438)
                     /gene="wecC"
                     /locus_tag="OHN27_001937"
     CDS             complement(12176..13438)
                     /gene="wecC"
                     /locus_tag="OHN27_001937"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCQ2286381.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13435..14565)
                     /gene="wecB"
                     /locus_tag="OHN27_001938"
     CDS             complement(13435..14565)
                     /gene="wecB"
                     /locus_tag="OHN27_001938"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCQ2286382.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14621..15667)
                     /gene="wzzE"
                     /locus_tag="OHN27_001939"
     CDS             complement(14621..15667)
                     /gene="wzzE"
                     /locus_tag="OHN27_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCQ2286383.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15679..16782)
                     /gene="wecA"
                     /locus_tag="OHN27_001940"
     CDS             complement(15679..16782)
                     /gene="wecA"
                     /locus_tag="OHN27_001940"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCQ2286384.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(17022..18281)
                     /gene="rho"
                     /locus_tag="OHN27_001941"
     CDS             complement(17022..18281)
                     /gene="rho"
                     /locus_tag="OHN27_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCQ2286385.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18366..18467)
                     /gene="rhoL"
                     /locus_tag="OHN27_001942"
     CDS             complement(18366..18467)
                     /gene="rhoL"
                     /locus_tag="OHN27_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCQ2286386.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18608..18937)
                     /gene="trxA"
                     /locus_tag="OHN27_001943"
     CDS             complement(18608..18937)
                     /gene="trxA"
                     /locus_tag="OHN27_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCQ2286387.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19068..20333
                     /gene="rhlB"
                     /locus_tag="OHN27_001944"
     CDS             19068..20333
                     /gene="rhlB"
                     /locus_tag="OHN27_001944"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCQ2286388.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20467..21951
                     /gene="gppA"
                     /locus_tag="OHN27_001945"
     CDS             20467..21951
                     /gene="gppA"
                     /locus_tag="OHN27_001945"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCQ2286389.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWSLEVH"
     gene            complement(21998..24019)
                     /gene="rep"
                     /locus_tag="OHN27_001946"
     CDS             complement(21998..24019)
                     /gene="rep"
                     /locus_tag="OHN27_001946"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCQ2286390.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24236..24684
                     /locus_tag="OHN27_001947"
                     /pseudo
     CDS             24236..24684
                     /locus_tag="OHN27_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            24883..25164
                     /gene="ppiC"
                     /locus_tag="OHN27_001948"
     CDS             24883..25164
                     /gene="ppiC"
                     /locus_tag="OHN27_001948"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCQ2286391.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25251..26726)
                     /gene="ilvC"
                     /locus_tag="OHN27_001949"
     CDS             complement(25251..26726)
                     /gene="ilvC"
                     /locus_tag="OHN27_001949"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCQ2286392.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     INLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26876..27769
                     /gene="ilvY"
                     /locus_tag="OHN27_001950"
     CDS             26876..27769
                     /gene="ilvY"
                     /locus_tag="OHN27_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCQ2286393.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNHK"
     gene            complement(27821..29365)
                     /gene="ilvA"
                     /locus_tag="OHN27_001951"
     CDS             complement(27821..29365)
                     /gene="ilvA"
                     /locus_tag="OHN27_001951"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCQ2286394.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIALHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTYWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29368..31218)
                     /gene="ilvD"
                     /locus_tag="OHN27_001952"
     CDS             complement(29368..31218)
                     /gene="ilvD"
                     /locus_tag="OHN27_001952"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCQ2286395.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31283..32212)
                     /gene="ilvE"
                     /locus_tag="OHN27_001953"
     CDS             complement(31283..32212)
                     /gene="ilvE"
                     /locus_tag="OHN27_001953"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCQ2286396.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32232..32495)
                     /gene="ilvM"
                     /locus_tag="OHN27_001954"
     CDS             complement(32232..32495)
                     /gene="ilvM"
                     /locus_tag="OHN27_001954"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCQ2286397.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32492..34138)
                     /locus_tag="OHN27_001955"
     CDS             complement(32492..34138)
                     /locus_tag="OHN27_001955"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCQ2286398.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34141..34191)
                     /locus_tag="OHN27_001956"
     CDS             complement(34141..34191)
                     /locus_tag="OHN27_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286399.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34278..34376)
                     /gene="ilvL"
                     /locus_tag="OHN27_001957"
     CDS             complement(34278..34376)
                     /gene="ilvL"
                     /locus_tag="OHN27_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCQ2286400.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8352 a         9485 c         8689 g         7915 t
ORIGIN      
        1 ataacacata tattaattgc cgttaaaact aaaaacagca tcaataatca acgcgatata
       61 ataaacctgc cttacatatc aactgcgcca gaggtaggat tgaaaacgct ctcctgattt
      121 tccaattcat tttctggata aataaataat ttatttttgt cactattatt tatgtaatca
      181 tcctgtcagg gagagggatc tcaattatca atgcttaatt acgtcatcat tttgatgaca
      241 tgaaaccgga gcattttgca tggaaatttc gttttcaccc aaacgtcttg ttgttgctgt
      301 cgccgccgct cttcctctca tggccagcgc agcagatacc ccgtcaactg ccaccgcacg
      361 caaaggcttt gccggatacg atcacccaaa ccagtatcca cagaacgctg caatttattg
      421 aatttgcacg atcatgtagg ccggataagg cgtttacgcc gcatccggca atcaaccgca
      481 ggcggccaca aaatggccgc ttttttactt actcaccacc agcaaatgcg catgcataat
      541 gtcactggcc gggcgaccgt gcgccagcaa atcagccatt gctttaagat atggcggtag
      601 atggcggaaa taacgctgat acccggcaca caaataattc agccccggtt tgccgctggc
      661 atcgagcatg aagcggtgtt tcgggcagcc tccccagcac gcttttaaca cgttacaact
      721 gcgacactgc gccggtaatt gcttaaattt atcttcacca aacgcctgct gttgcgggga
      781 atcgatcatt tctgcaattg tttgctggtg catattcccc agccgatatt gtggataaac
      841 atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca atcaccgagc gcccacaggt
      901 tggctgatga tggcaaaccg cacccggcgc accgacaaaa ttggcaaacg cccattcgat
      961 attcatcacg aaaatcttgc cgacgtcgcg tttaatccag tggtcgaata tcgccaccag
     1021 aaattcaccg aactcctcgg ggcgcaccga ccattccgtt agctcaccct gaatatcacc
     1081 aggcgcatgc aatttcagtc cttcgcgggc agccgtttca tcagccaggc gctcgaccac
     1141 cggaataaac tggatgaatt ccactcccgc atcgcacaaa aaatcatata cctgcaacgg
     1201 ttgctgcgcg ctggtgcgat taacgcagac cagcacgtta tagtcgacat gatgtttttg
     1261 cagcagcgtc agggcacgca tcaccagctt atgcgtgggt ctgccacctt tagtcacgcg
     1321 atattgattg tggatctcag gcggaccatc cagcgataaa ccaacaagaa aatgatgctc
     1381 cgcgagaaac gcgcaccatt cgtcatccag cagcacgccg ttagtctgga agctgttact
     1441 tatcttcctg ccagcaccat atttcgcctg tagcgctacg gcacggcggt aaaactccag
     1501 cccgagtagc gttggttcgc cgccctgcca ggtaaaagcc acttcgtttt gcagttcgct
     1561 ggcggcgata tagtgacgga tatacgcttc cagcgtgtca tcgtccatat gcgtgactgg
     1621 cttttcgcgg taaagggatt gtttttcgag ataaaaacag tagtcacagt tcagattaca
     1681 atcggaacca ctcggtttcg ccatcacatg aaaagcacgc gttggaacct gttgcagcat
     1741 ggtcactcct tgcctgaagt catccttcaa tacgttaagg gcgaaatcat accgtggggc
     1801 aatgagttat tacgggcgtt tattgcgaag ggagggtgac gaaatgcaca gaaaacaaaa
     1861 aaccccgccg aagcggggtt caaaattggt cggcgagaga ggattcgaac ctccgaccca
     1921 ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgaaatact gctttttgaa
     1981 tttttagttc aattctttaa agtcgtggtg cgaggggggg gacttgaacc cccacgtccg
     2041 taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc gcattgtcac
     2101 aacttctaat aatggggtgg ctaatgggat tcgaacccac gacaactgga atcacaatcc
     2161 agggctctac caactgagct atagccacca ctacaaatct tgttacgcgg tattactacc
     2221 accgcagctc aagcgccggg actaaatggc gcgcccgaca ggattcgaac ctgagacctc
     2281 tgcctccgga gggcagcgct ctatccagct gagctacggg cgcttagcgc cgttgcgggg
     2341 gtggataata cggacttccc acccctctgt ctagtccctt tttaaataaa ttgcgcgttt
     2401 ggttacggtt tgtgcgtttt gctgcttatt cctccagttt atgcgctttc ccgtggcgat
     2461 taaggccaaa aactttataa atcgccgtca ccgccagcat aaagatgatg ccaacaaaca
     2521 gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa aacgcaaatc aggaatgcca
     2581 ttgttacgta gttggcccac gggaacagga ttgagcggaa cggatggctg gcaatcgccg
     2641 ctttatgtgc acgccgaaaa cgcagctggc ttatcaatat cacaaaccat ggcaccatcc
     2701 ccggaagcac gctggcactg tagacgtaga caaacacacg ctgcggattg ggaatgatgt
     2761 agttcaggca tgagccaatt agcagaatag caatagatac tgccacaccc gcaaccggta
     2821 cgccgtgacg ggaaactttc gccatcgccg ccggtaactg acggtttttc gccagtgcgt
     2881 agagcatacg tccgcaactg tacatgccgc tgttacagcc agagagcgca gccgtcagca
     2941 ccacaaagtt gataatgccc gccgctgcgg taataccgat tttggcaaaa gtcagtacga
     3001 acgggctgcc gttgctgcca atttcattcc acgggaagat ggtgacgata acgaaaatcg
     3061 cgcctacgta gaaaatcagg atccgccaca gcaccttgcc tacggcactg cgcagtgtca
     3121 cctgcggatt cttcgcttca ccggcagtaa tgccaatcag ctccacgccc tggtaggacg
     3181 ccaccacaat acacagcgcg gtcaggaacc ctttccagcc acccgcaaag aaaccgccat
     3241 gctctgtgag attgctaaaa ccaatcgact gcccaccatt gccaaagcca aagaaaatca
     3301 cgcccaggcc aatgacaatc atcacgataa tcgtggtgac tttgatcatc gcgaaccaga
     3361 actcgatttc gccgtacaac cgcaccgccg ccagattcgc caacgccacc agcgccactg
     3421 cgatcaatgc gggtatccac tgcgccatct ccgggaacca gaactggaca taaacgccaa
     3481 tggcggtgat ttctgagatc cccacggcca tccacataaa ccagtaagac caggcggtga
     3541 gataaccaaa aaacgggctc atataacgat gcgcataaac ggcgaacgaa ccggtaaccg
     3601 gttcgaggaa caacatttcg cccattgaac gcatgatgaa aaagacgaac agcccggcga
     3661 tgatataggc caacaatacg gatggcccgg cccatttcag ggtactggcg gcccccataa
     3721 acaggccgac gccaatggtg ccccccaggg cgatgagttc gatatgtcga gcttccagcc
     3781 cacgctgtag ctctggtttg ttatctgcca taaatcctcg tgttgtgttt gcatgctttc
     3841 cggtgttacc ggttatcgtt atgggtacat cgagtgttgc gaatgttttc gtaattcagg
     3901 agaaatggca aataaagcat taaaaatttg aatgctttgt gtaataaaaa agcagacagg
     3961 cgacgaagtg accactccgt cgctttacaa agagaggaaa atcagaggtt gccggtgtaa
     4021 tgccagcgta aataacgcag caaacgaagc tgacgcttaa tgcggctcgg ctgcgaaagc
     4081 aggcggtaga gccactccag tcccagcgtt tgccagattt tcggtgcgcg ttttacgtga
     4141 ccggtgaaaa catcgtaagt cccgccaacg cccatataca gcgcatctgg atgcaccaga
     4201 cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca tcgcaacggt gacgatttgc
     4261 gctccgctgg catgaatgcg ttcaaacagc gcctgacgct gctcgggttt aaaataacca
     4321 tcctgactgc caacgatatt cacattccat tggttgcgca gtttagcttc agtttgcgcc
     4381 agcacttcag gtttaccgcc cacaagaaat accggcgtcc cttctttgcc tgcgcgcgcc
     4441 atcagctctt cccagagatc ggcaccggca acgcgggaaa cctgcgcctg cgggtacttt
     4501 ttacgtactg aacgtacaac gctgatgcca tccgcatatt taaattcggc agcgttaatt
     4561 aactccctga cctcggcgtt atcttcaatg gtcagcattt tttcggcatt aatggcaacc
     4621 agcgttccct gcttaagctg cccgtcagca aacagataat cgagggcatg ctgcatatca
     4681 cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg gtgccgtggt gttgttattc
     4741 attgttatcc ttcaacctgc gtccggagcg atgattttgt acgtttatga atgagtccgg
     4801 cgctttcaaa aagccagtac aacagttttg cgatcatcag acatgcgccg aagaccacga
     4861 taaaaaagac tacgcgtgag acaaacgaat ccagcccttc acgtgccagc acgatcatat
     4921 tgaagatcgc cccaaagcag aaactgtgca atatcgcagc cttatagcga ttagtctcgc
     4981 ggttgcccag ctcatacagc cagtcgaacc atttgatgat cagtccaacc acaatcgccc
     5041 cgagtgggat gaacaacgcg ccgcccatca ccaccagtga gcctataagc gtaggcgaga
     5101 tcgccagtcc ggagtggtta ttcagcactt cccaggtaaa gtagttggct gagttcagca
     5161 ccatactcgg gcgccccggc cacagccagg aagggataaa gacatagaaa tcgcggacaa
     5221 ttggagccag gccctggaag tcgatgttgt cgtagttctg caacagcaac gccagattct
     5281 cccacggcga gaaggtgtcg cgagtgagat agagaaacgt atagaacgct tcatcgccgc
     5341 tcacattcat tccatagcgt tttaacgcca gccagaacat gccaacaatc cccagcacgc
     5401 ccgccgccgc cagcatccac aacgaaatcc agccgcgaat aatgccaata aacaggaaga
     5461 tagcgaatgc gatgatgata ttggcgcgag tgccgccgac aatcatataa gtcagcaagc
     5521 caaaagcgac cgtgctgacg aggaaaaaca gccacgcttt gctgtcctgg cgcagaaagt
     5581 agaccaccag catcgccggg atgaaaaagt aaaagaagcg ttttaacgcc acgccggaga
     5641 cttcactgga aaagatctga ctgtaggagt taagccggaa cagcaaaaag ccgttgtgca
     5701 tgaagaagat gccgacgctt accagcgcga tgcccatcag gatcacccac gtaagattgg
     5761 tctccacgcg gttcatggta aacagcggac ggcgcggtac atcagcaacg cgtttgcgta
     5821 ggcgggtttt gtaggtgaca tagtaaaccg cgtagaagca gcccgcagaa agcaacgcct
     5881 gcaacaagat ttctggcggc gcgacaccaa catcaaagcg aaataccagc acgctggtca
     5941 gcgggaagcc gaagaaaaag gtgagcaaaa acagcaatga aaagaagaca ttgaagttaa
     6001 agcgcacacg gcgaaactca aaccaggtca gcgtggcaat aaacagcgtg cagagcagcc
     6061 agacaacaaa caggccactg aattgcagca gactcatgcg acctccccgg cggcaatcgc
     6121 caacgcccgc tgccagcctt gtagatagtt agggctaaag aaggcaatgg tgtttttatc
     6181 caccgacgcc aactggcgct gcgcttcacg cacaatatcc tcgttgagat cgtcggtagt
     6241 aaacagcacc ggcaaatgtt gttccgtcat atcctgccag aacggatttt cccggttaag
     6301 cacacaagga atgcccgcct gaatcagtaa gcacagcgta ccaatgccct gctggcgggc
     6361 aaaaataaag taaccaagat cgcactgacg aagtagcgcc agataggcgt caaattccag
     6421 tttttcgctc agaatttgta gattttcttc gctgaataac tccagccccg cctgacgaac
     6481 ttcctcaatg tacgcttcgt tattaggcgg atatcccatc ggcaccacca cttttaccgt
     6541 atcgccaaat tgctgatgaa cggcgcgcaa ggcagcaata tgctcattgc tgcggtcgcc
     6601 ggagttcccc accagaatgg tcattttccc ttcacgttgc cgatcgttcg ccatcgtatt
     6661 gagcgaaggg tccatccgcg tcgggaagta cagcagttcg ccccgcacct ttgggtgcgt
     6721 tttggcaaaa aagctcaaat cgccgcgggt ggcaaataca cagccgactc gcttttgcgc
     6781 caggcgacgt agtgggtaaa aaagcttata tctcaagcca ctggaaagct catacaggtc
     6841 tgccccccag atatgccaga aaaactggct gggcttaatg ccaccactca gcagagccag
     6901 ccacagtgtg ggattgaact gaccgtggaa gaaaaaacgc tgctgacggt tagcttttgc
     6961 tttcgcgatg accgcttccg ccagcgattt tttcccaggg aaaaattgca cagaaagcgc
     7021 cggacagcta tcacttaagc cgtcgtcctt gccaacaacc ataaactcgc gcgcgtgctc
     7081 gctcgtcgcg gccagcgcgt cattgaaaaa ccgcaaaacg gttcggttat ggtgagggat
     7141 atccgatccc agtacgtgaa tcagtacagt catgcccgcc tacgccagag taaaaacacg
     7201 ccacaacaaa gagaaaaata gacgatataa gttgccatat atgcctgcgc cgcgcccagt
     7261 gcaccatgcg cagggattag ccagtgggca aataccatca ataaagtgaa ctggctgact
     7321 tccgccagaa tataaaaccg cagtgacgct ttggcgatca ccagataacc aaagacataa
     7381 gcgcccactt ttaacacatc acccactaac tgccaggcaa agagatcgcg catagcggta
     7441 aatttattcg acaacagcag ccagatagca aaatcacgca gcagccagac ggtaaaactc
     7501 gccgccgcca ctgccggtaa gacgaatttc agcgatttaa ccacttcccg ggtgatatcg
     7561 cgcttttccg ttagccgcga caacgtgggc agcaaatata cgctgaacga tgccgtaata
     7621 aattgcaggt aggcatcgga aatactgctc accccttgcc agatccccac ctcatcccag
     7681 ctatactgcg ccgccagcag tttacgcatc atgatgtaag caacaggcaa ggtcaccgac
     7741 gtaatcaacg ccatgagcgt aaatttgctc aactgccctg ccagaccgtt atcccagctg
     7801 ggtttcagat agcttaacgg gatgacacca cgtttgatca acatgatggc ggcaggaatt
     7861 accaccagcg cgggaatcag cgccagaccc agcaacgccc cttcataacc gcccaaacgg
     7921 taactgacgt agtacgcgag aacgccaatc aagctgccga caatcagcga taacgcatta
     7981 cctgcggcat cgcgaaagcc tttcatcagc gccagtaaca ggttgcccca ggcgatcccc
     8041 atttgcacca gcgccactaa acgcaccagc ccctgatagt cggtattacc aaacaatccc
     8101 tggctgattg gcgcagctgc cagcacaaaa accagcgcca tcagcgtaga gaaaccaagt
     8161 accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt gcggattatc atggtactgg
     8221 gcaacgtatt tggttacacc gttaaagatg ccagccccgg caagcacgcc gagcacggta
     8281 atcaactggc ggaaatttgc cgccagccca agccccgccg gaccaaatga caccgccagc
     8341 aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg tactggccgc cgtccacaag
     8401 gacgcttttg ccaacgacat atcaggaaaa gtagttcaac aaagtcgcaa ttaccgtacg
     8461 ctgattgacg ggcgacaggt tgtagaacag cggcaggcgc agcaggcgct cgctctcttt
     8521 ggtggtgtag cgatcttcac cgtggaactc accaaagtgt tcccccgcag ggcaaccgtg
     8581 cagcggaatg taatgaaaca ccgccatgat ttccgcttct ttcagaaagt taatcaacgc
     8641 gctccggtca tcaatatccc gcagtttaat gtagaacata tgcgcgttct gcacgcagcc
     8701 atcgggaatc gacggcagct cgatacgccc ggctttcgcc agaggcgcta acgcatcgta
     8761 gtagttttgc cacagcgcca gacgttgctg gttgatacga tccgctgctt ccagttgcgc
     8821 ccacaggtat gcagcttgca gatcggacat caaatagctg gagccaatat cgcgccaggt
     8881 atatttatcg acctgaccac ggaagaactg gctgcggtta gtgccctttt cacggatgat
     8941 ctcggctcgt tcgattaacg ctttatcgtt aatcagcgtc gcgccgcctt caccgcccgc
     9001 cgtgtagttt ttggtttcat ggaagctaaa gcagccaata tgaccaatgg ttcccagtgc
     9061 acgccctttg taagtggaca tcacgccctg agcggcatct tctaccacaa acaaattatg
     9121 ctttttcgcc aacgccataa tggtgtccat ttcgcaggcc acacccgcgt aatggaccgg
     9181 cacgataacg cgcgttttgt cggtgatcgc cgcttcaatc agcgtttcgt cgatgttcat
     9241 ggtgtccggg cgaacatcca caaaaacgat ttttgcgcca cgcagcacaa aggcattggc
     9301 ggtggagaca aaggtgtagc tcggcatgat cacttcatcg ccaggctgga tatcgagcag
     9361 cagcgccgcc atctccagcg aagcggtgca ggacggcgtc agtaacactt tggcgctgcc
     9421 aaaacgttgc tccagccact gctggcagcg acgggtaaaa ccgccatcgc cacacagttt
     9481 gccgctaccc attgccgact gcatatagtc gagttcggtt cccaccaccg gcggtgcgtt
     9541 aaatggaatc atgtgatcac ctgtataacc agtacgcggt gctttctaca ttcgcaccac
     9601 tttgtatgta tcgtttaagc gcggcggtgt tgcccatttg ggtcgccacc cgcaaagttg
     9661 ttttaccgcg agcatacgcc cagtttagcg ccgtttgcat cagctcagca cctgcaccgc
     9721 gtccagccag caggccaatt cgcgcatctg tcgcattgag ttcccgtaaa gagacatagc
     9781 cgcgaatatc gccggacgcc gcacgtaaaa tcagacattg atgatcaaag gtgccgcgca
     9841 cggcattttc aatccactgt gcataaaagc gactgctggc gtcaggcgca taccacggcg
     9901 cacgaaaacg gctttgcgca aatgcggcgc tggctaactg acgtaatgcg ggaatatcgg
     9961 tctcttgtgc cactacagca ccgctatcac tggcattgtt cacgggtagc gccaaatcaa
    10021 cttcaccttc taccagggtg aatcccagct gttgcagggc atccagttca cccgtatttg
    10081 atgccgcaat tttggcctgc acccgtgacc acggcgctaa cgcgtctggc gtcaggagcg
    10141 gtgcttcaga cgtaatgcgc acgatggcgc tgttaacacc aaagaaggcg ttttcccagg
    10201 ttagtggctc aatactggcg cggacgggca cgaagtaact ccagcagata ttggccgtag
    10261 ccagttttcg ctaatgaact ggcagcacgc ttcacaccct cgtcatcgag ccagccgtta
    10321 cgccaggcaa tctcttccag gcaggcaatc ttaaagccct ggcgtttttc caccgtctgt
    10381 acaaaggtgc tggcttcaat caggctgtcg tgagtgccgg tatccagcca ggcaaatccg
    10441 cgcccgagca gttcaacggt caggttgccc gcctcgaggt acatctggtt gatggaggta
    10501 atctccagtt caccacgctc cgacggcttc acctgctttg cgtactccac gactttactg
    10561 tcgtagaaat aaagcccggt caccgcccag tttgacttcg gctgttttgg cttttcttcc
    10621 agcgagatag cgcggaaatt gtcgtcaaac tccaccacgc caaagcgttc cgggtccatc
    10681 acctgatagc caaaaaccgt cgccccttcg gtgcgcgccg caacatgacg cagcttcgga
    10741 ctgaagccct gaccgaagaa gatgttatcg cccagcacca gacaagaagg ttcaccatta
    10801 aggaaggttt caccgatgat aaaggcctgc gccagaccgt ccgggctggg ctgttcggca
    10861 tattccagct ggataccgaa ctcactacca tcgcccagca ggcgctggaa ataaccttta
    10921 tcttccggcg tagtgatgat gagaatttcg cggataccgg ccagcatcag caccgacagc
    10981 ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt gcttcgatac gccgcgcgta
    11041 atcggatgca atcgggtgcc ggaaccgccc gccaggataa tacctttcat gtttgcctcc
    11101 gctgaaaatt agcctttcag acctaaacgc tcgccctgat agctgccgtc ctgcacctgc
    11161 ttccaccagc tttcattagc cagataccac tgcaccgttt tacgcattcc actttcaaag
    11221 gtttcctgcg gcagccagcc aagttcacgg gcaattttcg aagcatcaat ggcataacgc
    11281 agatcatgcc ccggacggtc agcgacaaag gtgatcaagt cactatagtg caccacgccg
    11341 tgcggcttgt tcggagccag ttcttccagc agctcgcaaa tggtttccac aacatcgaga
    11401 ttcttacgct cgttgtgacc accaatatta taggtttcac cgactttccc ggtggtcgcc
    11461 acgcaataca gcgcgcgagc gtgatcttcc acatacagcc agtcacggat ttgctgcccg
    11521 ttgccatata ccggcagcga tttacccgcc agcgcgttga ggatcatcag cgggatcagt
    11581 ttttccggaa agtggtaagg accgtagtta ttcgagcagt tggtgataag cgtcggcaga
    11641 ccgtaggtcc gcagccaggc gcgcaccaga tggtcgctgc tggcttttga cgcggaataa
    11701 gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat catccgtcga gtgcaggtca
    11761 ccatatactt cgtcagtgga gatatgatga aaacggaacg ctgatttttt atcttccgtt
    11821 agcgcattcc agtaagcccg cgccgcttca agcaatgtat aagtcccgac aatgttggtt
    11881 tcaataaacg ctgccgggcc gtcaatagaa cggtcaacat ggctttcggc tgccagatgc
    11941 atgacacagt ctggctgatg ctcagtgaat acgcgtgcca gttctgcccg atcgcagata
    12001 tcaactttct caaaggcaaa gcgctcgctt tgcgcgaccg gtgccagcga catcaggttt
    12061 ccggcgtagg tcagcttatc gaccactacc accgcgtcgc tcgtttcgtt gatgatataa
    12121 cgcaccagcg ccgagccaat aaacccggca ccacctgtta tcagaatttt tctcatcagc
    12181 gccagactcc tttggcatcg acgacatact gctgatggac attgtcgcca ttgataactt
    12241 tgaactgact atgatcgacc agcatcacca gcacgtctgc cgttgccagc gcctcgtcaa
    12301 gctgcgccag agtacaaagc ccggtcagtt ttttcggcaa ctggtggatg ttaggctcaa
    12361 caaccagagt ttcgccgcta tgccactggg cgatcagttc agcgatttcc atcgccgggc
    12421 tttcgcgcag gtcatcaata ttcggtttaa acgccagacc aaagcaggcg attttcagtt
    12481 cactggcgcg tttatcggta gccgccaggc aatcagccac cgccgctttc acctgatcga
    12541 taacccagaa cggtttgtga tcgttcactt cgcgcgcggt acggataagc cgcgcctgct
    12601 gggggttctg tgccacgata aaccacggat caacagcaat gcagtgaccg cccacgccag
    12661 ggccaggctg aagaatatta acgcgagggt gacgattcgc caggcgaatc agttcccaga
    12721 cgttaatccc ctgatcggca caaatcagcg acaattcatt agcaaaagcg atattcacat
    12781 cgcggaagct gttttcggtg agcttacaca tttccgccgt ccgcgagtta gtgacgacac
    12841 actcaccttc gaggaaaatt ttgtacagtt cgctggcgcg ggccgaacaa accggcgtca
    12901 taccaccaat cacgcgatcg tttttaatca gctcgaccat tacctgtcct ggtaacacgc
    12961 gttccgggca gtaagcaatg ttgacgtccg cctgctcgcc cacctgctgc gggaaagtga
    13021 gatctggacg catctctgct aaccattctg ccatcttctc ggttgacccc accggcgagg
    13081 tggattcaag gatcaccagc gcgccttttt tcagcactgg cgcaatggag cgagcagccg
    13141 attcaacgta ggtcatatct ggctcatgat cgcccttaaa cggcgtgggt acagcaatca
    13201 gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa aaaaccgcct tctacggcag
    13261 tttttactac actcgccaaa tcaggttcga cgatatggat ttcgccacga ttgatggtat
    13321 caaccgcatg ttggttgata tcgacaccaa ttacctgttt ttgccgtgag gcaaacgctg
    13381 ctgccgttgg cagcccgata taacccagtc cgataacaga aatggtcgca aaactcatag
    13441 tgatatccga ttatttttta acgcttccag aatgcgagag catgcctgac catcaccata
    13501 cgggttatgg gcgcggctca tagcttgata ttcgttttcg tcttttaaaa gacgcgtcac
    13561 ttcctcgaca attcgctgct tatccgtgcc taccagacgc accgtacccg ccgtcaccgc
    13621 ttccggacgc tcagtggtat cgcgcatcac cagcacaggt ttccccagcg aaggcgcttc
    13681 ttcctgaatg ccgcctgagt cggtcaaaat cagccaggcg tggttcatca gccagacaaa
    13741 cggtaaatac tcctggggat cgatcagaat gacatttttc acatgcccca gaatgcgatt
    13801 gaccggctct ctgacgttcg ggttgagatg caccggatag acaatctgga tgtcctggtg
    13861 cgtggtggcg atgtctgcca gcgcgtggca gatttcttca aagccacgac cgaaactttc
    13921 acgcctgtga ccggtcacca gaatcatctt tttatcgggg tcgataaacg ggtaatttgc
    13981 cgccagttct gaacgcagcg tgtcgctgct catcacctgg tcacgcaccc ataacagtgc
    14041 atcaataact gtattaccag taatgaagat tcggctatcc gcaacgtttt cacgcagcaa
    14101 gttttgccgg gaagtttcgg ttggagagaa gtgatacatc gccagatgcc cggtcaatgt
    14161 acggttagcc tcttccggcc acggcgaata gagatcgccc gtgcgcagac cagcctcaac
    14221 gtgaccaaca ggaatacgct gataaaacgc cgccaggctg gttgccagcg tcgtcgttgt
    14281 gtcgccgtga accagcacga cgtctggttt gaactcggca agaataggtt ttagcccttc
    14341 cagaatccga caggttatct ctgtcaggcc ctgtcctggc tgcattatgt tgagatcgta
    14401 gtcaggtaca atggaaaaga gtttcagcac ctgatcaagc atctcccgat gctgcgcagt
    14461 gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac gcatgcacca acggagccat
    14521 cttgatggct tccgggcgcg taccaaatac agtcagtact ttcacatcga ttctcttcga
    14581 ataagcggtg agcgcctttg cgctcaccgc agcagtgttg ctatttcgag caacggcggg
    14641 ttaatgcgac accagccccg atcagccccc cgacaatgcc ccacataatc atcaggaagg
    14701 cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt acgcaaatag cgataggtct
    14761 gaaaacgcgg atccagggtt ggaccaacat tcagggtgtt taacatggcc cgattctgat
    14821 catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt ttccagtcga gcctggagca
    14881 ttggacgccc aagcaggaac atttctgaat caggtaattc ctcggcaggc acatctgtcg
    14941 cactgcgcga aatattatgc tgctcagcaa ttttcagcgc ctgttcaata ctgttcatcc
    15001 ggcggtcgta gatggctttc gccacctctt cctgacgctt cacctgtgct ttcatctgga
    15061 tggtacgcgc cgcccatgcg cctttcagct catcattcag atggctggct gcacgctggc
    15121 tggcaaaagc aacatactga cgtaacaggt tattagcgtc aggcgcggtt tcagcaataa
    15181 gcttcacgct gtcattgacc gcgcgggtaa agtctccggg gataaactgg atgttgttaa
    15241 tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc caccatccgc tgtttgtaat
    15301 agtcggtttg cagccagaac tctctgcggg tatcccacga ggccagttgc ataacaaact
    15361 ctttataggc ttcgtccatg accgatggtt ggtcggcaga agccatgttt gaacggacat
    15421 ccaggttacg caaaaattgc tgctgtgagt aatatccccc cagcatattc accgttggac
    15481 gatcggtaat cgccgtcgag ctccactcct gacgagcaaa aaacgtatac gccagcgcga
    15541 ttaacgcaaa cgccagcccc atgccaataa tccatagctt cccagcccac aaggtacgaa
    15601 acaacccacg aatatccagt tcattttcag cgtcttcggc cggtttccca ggcattggtt
    15661 gtgtcatcac atcctcattt atttggttaa attggggctg ccaccacgat ttctacgcag
    15721 tctgcgtttt acgcgcttaa taaagcgagc aactttccag gcacgcttaa tgcaatatcc
    15781 atagaggaag aatgctagca aaaagagcac cagcatgacc cactccggga caaaatgaga
    15841 atattctgcc agcacgccaa tggaagcgag cagtgctgcg gcaagggtaa tcagcacaaa
    15901 cgcctgacgg gaagtaaacc cggcacgcat gatcaaatgg tgaatatgct gacggtcagg
    15961 agagaatggg ctcatgcctt tacgcaggcg acggtacata atcgccacca tatccattag
    16021 cggaatggcg attatccaca aagcggtaac cgggctgatg ggatgggttt tgccctgggt
    16081 cgtttcgagc aggatccaga taacggtaaa accaatcagc gtactgcccg catcacccat
    16141 aaagactttg tagcggcgac ccaggatacc aaggttaagc atgatgtatg gcaggatggc
    16201 ggcgatcatc gcaaagcacc agattgcgag gctggtttgc ccgtcgaacc acaaaatcat
    16261 accgattgct gcaaacgaga cgcaggacaa cccgcccagc aagccatcaa tgccatcaac
    16321 catgttgaac gcattaatgg ccgcccagac ggcaaatagc gtcaggaagt aaccaaacgg
    16381 tccgagcacc atctcccagg agccaaagat ataacccagg ctactgagat aaaggttgcc
    16441 gaacaccatc ataacaatgc caacagccgc ctgtatggtg gcacggattt ttacgctgat
    16501 atcaaaacgg tcatccagcg cgccaatgaa aacaagcaca ccggcacaag cgagatagag
    16561 agatgcatgc ggaatatagt aatcgacaat tccgaacgtg aagcaaatcc ctgcgtaaac
    16621 cgaaataccc ccaacgagtg gtatcaaccc ctggtgacgt ttgcggaagt ttggtttatc
    16681 cactaaaccg actttttttg ccaccttacg ggcaaaaaac agaaacagtg tcgtgaataa
    16741 aaaaatactg atgagatcag tactcactgt cagtaaattc acaatgcatg ttctcagaga
    16801 aaattattag cagaagtata accacgaaga cctttattca gaagggaaag tccgttccaa
    16861 acccaccagt cctgtgcaaa aattaaccat ttgatatgca aattgcataa catgccagca
    16921 aattccagga attttcctaa catcgccagc gcggcattaa gattacagat ataaaagcaa
    16981 aacgccacgt aaacacgtgg cgtttttggc ataagacaaa tttatgagcg tttcatcatt
    17041 tcgaagaaat cgtcattggt cttggtcatt gccagtttat taatgaggaa ttccattgca
    17101 tcgatttcgc ccatcgggtg aatgattttg cgcaggatcc acattttctg cagttcttcc
    17161 tgagtcgtga gcagctcttc tttacgggta ccagaacggt tgtagtcgat agccgggaag
    17221 acgcgttttt cagcgatctt acgagagagg tgcagttcca tgttgcctgt acctttaaac
    17281 tcttcgtaga taacttcgtc cattttagaa ccggtatcga taagcgccgt cgcgataatg
    17341 gtcaggctgc cgccctcttc cacgttacgc gccgcaccaa agaagcgttt cggacgatgc
    17401 agggcgttgg catccacacc accggtcaac actttacctg acgccggaac aacggtgttg
    17461 taagcgcgcg ccagacgagt gatggagtcg agcagaatga taacgtcttt cttgtgctca
    17521 accaggcgtt tggccttctc gatcaccatt tccgcaacct gaacgtggcg agatgcgggt
    17581 tcgtcaaagg tagaagcaac aacttcacct tttaccagac gctgcatctc ggttacttct
    17641 tccggacgtt cgtcgatcag cagaaccatc agcacacaat ccgggtggtt gtaagcaatg
    17701 ctctgagcaa tgttctgcag cagcatggtt ttaccggctt tcggcggtgc cacaatcaga
    17761 ccacgctgac cacgaccgat aggtgatgcc agatccagta cgcgagcagt taaatcttca
    17821 gtagaaccgt taccacgttc catacgcaga cgagagtttg cgtgcagcgg ggttaagttc
    17881 tcaaagagga ttttgttgcg ggcgttttca ggtttgtcga agttaacttc gttaactttc
    17941 agcagcgcaa aatagcgttc accttctttc ggcgggcgaa tcttaccaga gatggtatca
    18001 ccagtgcgga ggttgaaacg gcggatttgg ctaggggaaa cgtagatgtc atcaggaccg
    18061 gcgaggtagg agctgtctgc ggaacggagg aaaccaaatc catcctgcaa tatctccagt
    18121 acgccatcac caaagatatc ttcgccactc tttgcgtgct gcttcaggat ggcaaaaata
    18181 atgtcctgct tacgcatacg agccaggttt tccagcccca tattttcgcc gagagtgatc
    18241 agctcagaaa ccggcgtatt ctttaattcg gtaagattca tagtggtgtg agttcttaaa
    18301 cttggggtaa atctcgaact taatgttgtg aatggtatgg caggatcatc catgcctgtt
    18361 tacggtcatc gactcatgtc ttttcgctgc ctggtcacag gagagtacgc agaactgaaa
    18421 cgacaagacg gattgagtga cgagccagaa atctgttcac ttcgcattta agataaaacg
    18481 ggaagcgttg gaaattacaa gattcaaact taataaggta tgtttaatac gaagtcaaca
    18541 ctaagttagc atgactcacg ccgggcgtcc agtttttagc gacggggcac ccgaacatga
    18601 aattccctta cgccaggtta gcgtcgagga actctttcaa ctgaccttta gacagtgcgc
    18661 ccactttggt tgccgccact tcaccgtttt tgaacagcag cagagtcggg ataccacgga
    18721 tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca acggtcagtt
    18781 tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg cacggaccgc
    18841 accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca tccgtgtcaa
    18901 aactgtcgtc agtcaggtga ataattttat cgctcatata taactccaca ggaataagcc
    18961 tggcgtgttg gtttcgttgt tggtgtaaca ttaaccaact aaaggttgac tttatttcac
    19021 cggatacgct ttcgtaaagc aatagtaagc tgatattcta ccacactatg agcaaaacac
    19081 atttaacaga acagaagttt tccgacttcg ccctgcatcc gaaggttgta gaagcccttg
    19141 aaaaaaaagg gtttcataac tgtacgccca ttcaggcact ggcccttccg ctgacgctgg
    19201 cgggtcgtga cgtagccggg caggcgcaaa ccggtaccgg gaaaacgatg gcgtttctta
    19261 cgtcaacgtt tcattatctt ctctctcatc ccgcgattgc cgatcgcaag gtgaatcagc
    19321 cgcgtgcctt aattatggca ccgacgcgtg aacttgccgt gcagatccat gccgacgcag
    19381 aaccgctggc ggaagctact ggcctgaagc tgggtctggc ttacggtggt gatggctacg
    19441 acaaacagct gaaagtgctg gaaagcggcg ttgacattct gattggcacc acggggcgtt
    19501 taattgacta cgccaagcag aaccacatta acctcggtgc cattcaggtg gtggtactgg
    19561 acgaagccga tcgcatgtac gatctgggct ttattaaaga tatccgctgg ctgttccgcc
    19621 gtatgccgcc tgcaaaccag cgcctcaaca tgctgttctc tgccacgctt tcgtaccggg
    19681 tacgtgaact ggcgtttgag cagatgaaca atgccgaata tattgaagtg gaaccggaac
    19741 agaaaacggg ccaccgtatt aaagaagagc ttttctaccc ttctaacgaa gaaaaaatgc
    19801 gtttgctgca aacgctgatc gaagaagagt ggccagacag agcgattatt ttcgccaata
    19861 ccaaacaccg ttgtgaagag atctggggcc acctggcggc agatggtcat cgtgtcggtt
    19921 tgttgacagg cgatgtcgcg cagaaaaaac gtctgcgtat tcttgatgaa tttacccgtg
    19981 gcgatctgga tattctggtt gccaccgacg ttgccgcgcg tggtttgcat attccggcag
    20041 tgacgcacgt ctttaactac gatttacccg atgactgtga agattacgtt caccgtattg
    20101 gtcgtacagg tcgcgcaggc gcaagcggtc actctatcag cctggcgtgt gaagagtatg
    20161 cattgaattt gcctgctatt gagacctata ttggtcactc aattccggta agcaaataca
    20221 atccggacgc attgatgacc gatctgccaa aaccgctgcg cctcacgcgc ccacgtacag
    20281 gcaatggtcc gcgtcgtact ggcgctccgc gtaatcgtcg tcgttcaggt taaataaaat
    20341 gtcatactca aaatcattca aactgtatca agatggcaag agaatgaatc ccgatgagca
    20401 tactgtagta agtgattcag gtgaaagaat gcagccaaca cagagacaga ttgaaggatg
    20461 aagagtatgg gttccacctc gtcgctgtat gcagccattg atctcggttc gaatagtttt
    20521 catatgctgg ttgtgcgcga ggtggctgga agcatccaga cgctgacgcg aataaaacgc
    20581 aaagtgcgtc tggctgctgg cctgaatagc gaaaatgccc tgtctaatga agcaatggag
    20641 cgcggttggc aatgtctgcg cctgtttgct gaacgtctgc aagatatccc tccctcgcaa
    20701 attcgcgttg tcgctacggc gacattacgt cttgccgtca atgcgggtga ttttattgcc
    20761 aaagcgcagg aaatcctcgg ttgtccggta caggtgatca gcggtgaaga ggaagcacgt
    20821 ctgatttatc agggcgttgc tcacacaact ggcggtgccg atcagcgcct ggtggtggat
    20881 ataggcggtg ccagtactga actggtaacc ggcacgggtg cacaaaccac ctcgttgttc
    20941 agcctgtcga tgggctgcgt tacctggctg gaacgctatt ttgccgatcg taatctgggg
    21001 caggaaaatt ttgatgctgc agaaaaagcg gcacgcgaag tgttacgtcc ggttgccgat
    21061 gaattacggt atcacggctg gaaagtgtgc gttggtgctt ccggcaccgt gcaggcgtta
    21121 caggaaatca tgatggcaca ggggatggat gaacgcatta ccctggaaaa gttgcagcaa
    21181 ttgaaacagc gagccattca ttgcggtcgg ctggaggaac tggagattga cgggctgacg
    21241 ctggaacgtg cgttagtgtt cccgagtggt ctggcgatcc tgattgccat ttttaccgaa
    21301 ctgaatattc agtgtatgac cctggcgggc ggtgcgctgc gtgaaggtct ggtctacggt
    21361 atgttgcatc ttaccgtcga gcaggatatt cgcagccgta cgctgcgtaa tattcagcgc
    21421 cgctttatga tcgatattga tcaggcgcag cgcgtagcca aagttgcggc taacttcttc
    21481 gatcaggtgg aaaaggaatg gcatcttgaa gcaataagcc gcgatttgct catcagcgcc
    21541 tgtcagcttc atgaaatcgg cctgagcgtt gacttcaaac aagcgccgca acacgctgct
    21601 tatctggtgc gtaatctgga tcttcccggt tttacccccg cacagaaaaa attgctggcg
    21661 acgctactgc tcaaccagac taatccggtc gatctctcat cgctgcatca gcaaaatgcc
    21721 gtaccgccgc gcgtcgcaga acaactctgc cgtttactcc gcctggccat catttttgcc
    21781 agccgtcgcc gtgacgatct cgtgccagag atgacattac aggctaacca tgaactgttg
    21841 accttgacgc ttccgcaagg ttggctaacc caacatccgc tgggtaaaga gattattgct
    21901 caggaaagcc agtggcagag ctatgtccac tggtcgctag aagtgcatta atgagtaagt
    21961 gccggatgcg atgctgacgc atcttctccg gccttgatta tttccctcgt tttgccgcca
    22021 tcatcgcttt cagattcgcc agatggcttt gccctttctg catccgttct tcggcgctga
    22081 ccactttgcg ctcctgttcc caaatcagat catcctgcgg cagctccagc aaaaagcggc
    22141 tcggctccgg gcgcaccagc tcgccgtact gacggcgttc tttacacagc gtaaaggtca
    22201 attccttctg ggcgcgggta atgccgacat aggccagccg ccgctcctca tcgatattat
    22261 cttcatcgat gctgctctgg tgcggcaaaa acccttcttc cataccgacc atgtagacat
    22321 aaggaaactc cagccctttt gacgcgtgca gagtcatcag ttgcacctga tccagctctt
    22381 cttcactctc accacgctcc atcatgtcgc gcaaagtaaa gcgcgtcacc acctgggtga
    22441 gcgtcatcgg ctcatccagt tcgctgcctt ccagcatctc cgtcatccag ctaaacagtt
    22501 ggttgacgtt cttcatgcgc atttcggcag ctttcgggct gggcgatgtt tcgtacagcc
    22561 aggattcata atccatgccg tggatcagat cgcgcaccgc ggcaatcggc tcccgctccg
    22621 ccagacgctg aatttctgcc aaccagtgag tgaagcgggt caatgcttca taaccacgtc
    22681 cgctaagcgt ctgactcagg cccatatcaa agctggcggt aaacatgctt ttattgcgcg
    22741 tcatcgccca ctcacccagc tttttcagcg tagccgggcc aatctctcgc ttcggcgtgt
    22801 taacgatacg cagaaatgcg ctgtcatcgt ccgggttagt cagcacgcgc agataagcca
    22861 gcaagtcctt gatttcagga cgagagaaaa acgacgtacc accagaaatt ttgtacggga
    22921 tgcggttttg catcaggaat ttttcaaaca cccgcgactg atggttaccg cgataaagaa
    22981 tggcgtaatc tttgtactgc gttttattga cgaagtgatg ggcgatcagc tcgccagtaa
    23041 cgcgctcggc ttcatgttcc tcgttattcg cgcttaaaac ttttagctct gtgccgtaac
    23101 ccagttcgga gaacagacgc ttttcaaaga cgtgcggatt attggcgatc aggatgttcg
    23161 ccgctttcaa aatacgtccg gaagagcggt agttctgctc aagcttaatc accttcagcg
    23221 ccggaaaatc ctgactcagc agcaccaggt tttgtggacg tgcaccgcgc caggagtaga
    23281 tcgactggtc gtcatcgccc accacggtaa agcgcgcgcg gctgcccacc agcagtttca
    23341 ccagttcata ctggctggtg ttggtgtcct gatactcatc caccagcagg tagcgaattt
    23401 tgttctgcca gcgctcgcgg acttcttcat tgcgttgcag cagcaacgtc ggcaataaaa
    23461 tcagatcatc gaagtcgaga acgttacagg ctttcaggtg tgcatcatac agcccataac
    23521 aatgggcaaa aatacggtcc cgctcgccaa tcgcactggc tgccgcctgg gacggtgttt
    23581 tgagatcgtt cttccagtta gagatggtcg aaatcaactg ttgcaggaga actttgtcat
    23641 cttcaatcag cccttcggtc aactctttga gcaacgccag ctgatcggta tcgtcaaaca
    23701 gcgaaaagtt cgccttcatc ccaagcgccg catactcgcg tttgatgata tccaacccca
    23761 gcgtgtgaaa agtggagatc atcagcccgc gcgcctcttt gcgacccagc gtctgcccga
    23821 cacgctcttt catctcgcgc gccgccttat tggtaaaggt caccgccgcg atatgtcgcg
    23881 cctgataacc gcaaccgcgg atcagatggg cgattttatt ggtgatcaca cgagttttac
    23941 cggaacccgc gcccgccagc accaggcagg ggccggtaac gaattcgaca gcttgttgtt
    24001 ggccggggtt tagacgcata ggtgtattgc tcaatcttcg aacgggggga ggattgtagc
    24061 agaatcgcat ttgaatgtga ccgcgtgtaa gccgctaacc ggttggctgc ggatgtttaa
    24121 atcagagaga gaagaccgaa atcggagttc atcgtgaatc cattagactt agaaaatatc
    24181 gggtcgtaac gttgtgttat tactttttca gagtttacta aggagaggtt tactcgtggc
    24241 gattaatttc agccctaagg ttggtgaaat actggaatgc aactttggta actatcctgt
    24301 tagccaaaat ggaccgttca gcacgacgta ttacgatggc cgcattcctc ctgaaatgat
    24361 aaaaaacagg ttagttgtcg tgctcaacgg aaaaattaat ggcaacgcat gcatcgtcgt
    24421 tccattatca accactcgcg accagacaag ttaaaacgtg gaatgcatgt tgaaatagcc
    24481 agtaatgtta tcaatgacct gcaattcttt gatcagcaga ttcgttgggc caaagccgac
    24541 ctggtacaac aagtcagtcg aaacagatta aacagagcaa ggacttacag agggtatctt
    24601 aatcaatgtt tgccacatga gttggttgca gacatacaga gggcggtcat caagtcaatc
    24661 aatgccatat cgctcataaa ttgacaataa aataaccatc cccgataatg actttgtagc
    24721 ccatagaggc cctaagtttt ctccccttga gggacctgcg aagcccggat tttccgggct
    24781 tttgccatat atgtaccagc cactccctga ctgacaatta cgttagcatc tgcggatcat
    24841 gctaaaatcg ccgcctgaca attattcact caaggcacga tcatggcaaa aacagcagca
    24901 gcactgcata tccttgtaaa agaagagaaa cttgctctgg atcttctcga gcagattaag
    24961 aacggggccg atttcggcaa gctggcgaag aaacactcca tttgcccatc aggcaaacgc
    25021 ggcggtgatt taggtgaatt ccgccagggt cagatggttc cggcgttcga taaagtggtt
    25081 ttctcttgtc cggtactaga gccgaccggc ccgctgcaca cccagttcgg atatcacatc
    25141 attaaggtgc tgtaccgcaa ctaatagcaa ggccttctcc aggagaaggc cttgagtgtt
    25201 ttctccctct ccctgtggga gagggtcggg gtgagggcat cagcgcgcac ttaacccgca
    25261 acagcaatac gtttcatatc tgtcatatag ccgcgcagtt tcttacctac ctgctcaatc
    25321 gcatggcagc gaatcgcttc gttcacatca cgcagttgcg cgttatctac cgcaccttcc
    25381 ggaatagctt tacccagatc gcccggttgc agctctgcca taaacggttt cagcagcggc
    25441 acacaagcgt aagagaacag atagttaccg tactcggcgg tatcagagat aaccacgttc
    25501 atttcgtaca gacgcttacg ggcgatggtg ttggcaatca gcggcagttc gtgcagtgat
    25561 tcatagtaag cagattcttc gatgatgccg gaatcaacca tggtttcgaa cgccagttca
    25621 acgcccgctt tcaccatcgc gatcatcagt acgcctttat cgaagtactc ctgctcgccg
    25681 attttgcctt catactgcgg cgcggtttca aacgcggttt tgccggtctc ttcacgccag
    25741 gtcagcagtt tcttatcatc gttggcccag tccgccatca taccggaaga gaattcgccg
    25801 gagatgatgt cgtccatatg tttctggaac aggggtgcca tgatctcttt cagctgttca
    25861 gaaagcgcat aagcacgcag tttcgccggg ttagagagac ggtccatcat cagggtgatg
    25921 ccgccctgtt tcagtgcttc ggtgatggtt tcccaaccga actgaatcag tttttctgcg
    25981 tatgctggat cggtaccttc ttccaccagc ttgtcgaagc acagcagaga gccagcctgc
    26041 aacataccgc acaggatggt ttgctcgccc atcaggtcag atttcacttc cgcaacgaag
    26101 gacgattcca gcacacccgc acggtgacca ccggttgcag ccgcccaggc tttggcaatc
    26161 gccatgcctt cgcctttcgg atcgttttcc gggtgaacgg caatcagcgt cggtacgccg
    26221 aacccacgtt tgtactcttc acgcacttcg gtgcctgggc atttcggcgc aaccatcact
    26281 acggtgatat ctttacggat ctgctcgccc acttcgacga tgttgaaacc gtgcgagtag
    26341 cccagcgccg cgccgtcttt catcagtggc tgtacggtgc gcactacatc agagtgctgc
    26401 ttgtccggcg tcaggttaat caccagatcc gcctgtggga tcagttcttc gtaagtaccc
    26461 actttaaaac cattttcggt cgctttacgc caggacgcgc gcttctcggc aatcgcttct
    26521 ttacgcagag cgtaggagat atcgagacca gaatcacgca tgttcaggcc ctggttcaga
    26581 ccctgtgcgc cacagccgac gatgactact tttttaccct gaaggtagct cgcgccatcg
    26641 gcgaattcat cgcggcccat aaagcgacat ttgcccagct gtgccagctg ctggcgcaga
    26701 ttcagtgtat tgaagtagtt agccatggtg attcctcgtg atgttgtgct tcttattgtt
    26761 cggttcgcgt ttgcgagatt gaattcacta tatgacagga aatttattgc ggaaattgat
    26821 atattcacaa cgtcacattg caatttttgc aacgtcaaca tcgagggctg tccctgtgga
    26881 tttacgcgat ctgaaaacct tcctgcatct ggcggaaagc cgccattttg gccgcagcgc
    26941 gcgggcgatg cacgttagcc catccacgct ctcacggcag attcagcgcc tggaagaaga
    27001 tctcggtcag ccgctgtttg tgcgcgataa ccgcacggtg acgctgactg aagcgggcga
    27061 agagctgcgc gttttcgccc agcaaacgct gttgcagtat cagcagttgc gccacaccat
    27121 cgatcagcaa gggccgtcgc tctctggcga attacatatc ttctgctcgg tgaccgctgc
    27181 ctacagccat ctgccgccga ttctggatcg cttccgcgcg gaacacccgt cggtggagat
    27241 taaacttact actggtgatg cggcagatgc gatggaaaag gtggtcactg gtgaagcgga
    27301 tctggcgatt gcgggtaaac cggaaacctt gcccggcgca gtggcgtttt cgatgctgga
    27361 gaatctggca gtagtgctga ttgcccccgc gctgccctgc ccggtgcgta atcaggtgtc
    27421 ggtagagaag ccggactggt caacggtgcc gtttattatg gccgatcagg ggccggtacg
    27481 ccgccgcatt gaactgtggt ttcgacgcaa taaaatcagt aacccgatga tttacgccac
    27541 ggttggcggg catgaagcga tggtatcgat ggtggcactc ggctgtggcg tggcattgtt
    27601 gccggaagtg gtgctggaaa acagccccga accggtgcgt aaccgcgtga tgattttaga
    27661 gcgcagcgat gagaaaacgc cgtttgagct gggcgtgtgt gcgcagaaaa agcggctaca
    27721 tgagccgcta attgaggcat tctggaagat tttgccgaac cacaaatgac gttgtcgcgc
    27781 gggtaggcct gataagcgaa gcgctatcag gcatttttcc ctaacccgcc aaaaagaacc
    27841 tgaacgccgg gttattggtt tcgtcgtggc aatcgtagcc cagctcattc agccgggttt
    27901 cgaaatccgg ttcatggtcg ccaagttcga acgccgccag tacgcgcccg tagtcggtgc
    27961 catggctgcg atagtggaac aaagaaatgt tccagtacgt acccagcgtg ttgaggaagc
    28021 gcagcagcgc gcccggtgat tccgggaatt cgaagctgta gaggcgttcc tgcaacggat
    28081 gcgatggacg tccgccgacc atatagcgca cgtgtagctt cgccatttcg tcgtcggaga
    28141 gatcaaccac gctgtagccg ccgtcgttga gcatctgcaa aatttctttg cgctcttcga
    28201 ggccgcggct caggcgcaca ccgacaaaga tgcaggcgtt tttggcatcg gcaaaacggt
    28261 agttgaactc ggtgaccgaa cgcccgccaa gcagttggca gaatttgagg aagctgcctt
    28321 tttcttccgg aatggtcacc gccaacaacg cttcacgctg ttcgcccagt tcgcagcgtt
    28381 ctgagacgta gcgcaggccg tggaagttca cgttggcacc ggaaagaata tgcgccagcc
    28441 gttcgccgcg aatgttgtgc agggcgatat attttttcat tcccgccagc gccagcgcgc
    28501 cagagggttc cgccaccgcg cgcacatctt cgaataaatc cttcatcgcc gcacagatcg
    28561 catcgctatc gacggtgatg atgtcgtcga gatactcctg gcataaacgg aaggtttcgt
    28621 caccgatgcg ttttaccgct acgccttcag caaatagccc tacgcgcggc agatcaaccg
    28681 gatgacccgc atccagcgct gctttcaggc aggcggagtc ttccgcttct acggcgatca
    28741 ctttgatttg cggcatcagt tgtttgatca gcaccgccac gccagcagcc agaccgccgc
    28801 cgccgactgg cacaaatacg cggtcgagat gggcgtcctg ctggagcagt tccagcgcca
    28861 gcgtgccttg cccggcaatc accatcggat ggtcgaacgg cggcacccag gtgaacccct
    28921 gctgctgtga cagttcgatc gctttggctt tcgcttcatc aaagttcgcg ccgtggagca
    28981 gcacttcgcc gccgaagccg cgcaccgcgt cgactttgat gtcggcggtg gcggttggca
    29041 taacgatcag ggccttcacg cctaaccgcg cagaagaaaa cgcgacgccc tgcgcgtggt
    29101 tacccgcaga agcagtgatc acgccgtgcg ctttctgttc ttccgtcagg cccgccatca
    29161 tggcgtatgc gccgcgcagc ttaaagctgt gcactggctg gcgatcttcg cgcttcacca
    29221 gaatgacgtt atcaagacgc gacgacagtt tttccatttt ttgtagcggc gtaacctgcg
    29281 ccgcctcgta aaccggcgcg cgcagcactg ctcttaaata ttcggcacct tccggagcac
    29341 cggacagggg ttgcgagtca gccattatta accccccagt ttcgatttat cgcgcaccgc
    29401 gcctttgtcg gcgctggttg ccaggctggc ataagcacgc agggcaaagg agacctgacg
    29461 ttcacgattt ttcggcgtcc aggctttgtc acctcgagcg tcctgcgctt cacgacgcgc
    29521 cgccagttcg gcatcgctta cctgtaactg aatgccacgg ttcgggatgt cgatagcgat
    29581 caggtcacca tcttcaatca ggccaatgct gccgccgctt gccgcttccg gtgagacgtg
    29641 gccgatggaa agaccagagg tgccaccaga gaaacgaccg tcggtgatca gcgcacaggc
    29701 tttgccgaga cccattgatt tcaggaagct ggttgggtag agcatttcct gcatccccgg
    29761 accgcctttc gggccttcat agcgaattac taccacatct ccggcgacaa ctttaccgcc
    29821 gagaatcgct tctaccgcat cgtcctggct ttcgtacact ttcgccgggc cggtgaattt
    29881 gaggatgctg tcatcgacgc ctgccgtttt cacgatgcag ccgttttccg caaagttacc
    29941 gtagagcacc gccaggccgc cgtctttgct gtaggcgtgt tccagcgagc ggatacagcc
    30001 attggcgcga tcgtcgtcca gcgtatccca acggcaatct tgcgagaatg cctgtgtggt
    30061 acgaatgcct gcaggacctg cgcggaacat attttttacc gcgtcatcct gggtcagcat
    30121 aacgtcgtat tgttccagcg tttgcggcaa cgtcaggcca agtacgtttt tcacatcacg
    30181 gttcagtaac cccgcgcgat ccagttcgcc gagaataccg ataacaccac cagcacggtg
    30241 aacatcttcc atatggtatt tctgggtgct cggcgcaact ttacacagct gtggaacctt
    30301 gcgggaaagc ttatcgatat cactcatggt gaagtcgatt tccgcttcct gcgccgccgc
    30361 cagcaggtga agtacggtgt tagtcgatcc acccatcgcg atatccagcg tcatggcgtt
    30421 ttcaaacgcc gccttactgg cgatattacg cggcagtgca ctttcgtcgt tttgctcgta
    30481 ataacgtttg gtcaattcaa caatgcgttt accagcatta aggaacagct gcttacggtc
    30541 ggcgtgggtt gccagcagcg agccgttgcc cggctgcgac aggcccagcg cttcggtcag
    30601 gcagttcatt gagttagcgg taaacatccc ggagcaggaa ccgcaggtcg gacacgcgga
    30661 acgttcaacc tgatcgctct gggagtcaga tactttcggg tctgcgccct ggatcatcgc
    30721 atcaaccaga tcgagcttga tgatctgatc ggaaagtttg gttttcccgg cctccatcgg
    30781 gccgccggaa acaaagatca ccggaatatt caggcgcagg gaagccatca gcatccccgg
    30841 ggtgattttg tcgcagttag agatgcagac catggcgtcg gcgcagtggg cgttgaccat
    30901 atactcaacg gaatcagcga tcagttcgcg agatggcagt gaataaagca tccccccgtg
    30961 gcccatggca atcccatcat ccaccgcaat ggtgttgaac tctttggcaa cgccgccagc
    31021 cgcttcaatt tgttcggcga ccagtttacc gagatcgcgc agatggacgt gacccggtac
    31081 aaattgggtg aacgagttca caaccgcgat aatcggcttg ccaaaatcgg catcggtcat
    31141 tccggtggcg cgccacagcg cacgagcacc cgccatatta cgaccatgag tggtggtggc
    31201 ggaacggtac ttaggcatac tttatttact cccagtgtct gtctcgtaaa tgggacggtg
    31261 cgtgccgtcc cattttttgt atttattgat taacttgatc taaccagccc catttatctt
    31321 cggtttcgcc agtgaagagg ccgaagaagg cttgctgaat gcgtttggta accggaccgc
    31381 aacggccttc accaacctga ataccatcta cgctgcgcac tggcgtgatt tctgccgcag
    31441 taccggacat aaacacttca tccgccaggt acagtgattc gcgcgacaga acctgctcac
    31501 gcacttcaat tcccagctct ttcgccagtt tgatgatggc atcacgggta atacccggca
    31561 gcgcggagga ggtgaacggt ggggtgaaca gcacgccgtc tttcacttca aacaggtttt
    31621 cgcctgcacc ttcagagatg tagccattca catccagcgc gataccttcc tgataaccgt
    31681 ggcggcgcgc ttcgctaccc accagcaggg aagagaggta gttaccaccg gcttttgccg
    31741 ccgtcgggat ggtgtttggt gctgcgcggt tccaggagga aaccatcgca tcgatcccct
    31801 gctccagcgc ttctgcgccc agatacgctc cccacgggaa agcagcgata atcacgtcgg
    31861 ttgagtatcc cgctggcggg tttacgccca tgccaacatc accaacgaag atcagcggac
    31921 ggatataggc gctggtgaga ttgtttttgc ggatcacgtc acgacaagct tccatcagct
    31981 catcaatgct ctgcgaaacc gggaagcgat agattttggc ggagtcatgc agacgctgca
    32041 tatgctcacg atggcggaat acaaccggtc ctttgtgcga gtcgtagcaa cggatgcctt
    32101 caaaaaccga ggtgccatag tgcagcgcgt gcgacatcac atgcaccttc gcgtcttccc
    32161 agcgaaccat ctccccattg aaccaaatgt aatcagcttt cttcgtggtc atttttatat
    32221 tccttttgcg ctcaggcgcg gatttgttgt gatgtggttg tgctctggca gatggcaacg
    32281 tgtgcgacgt ccaccagttt atttaactga ctaaacagta agtcgaccga ccgtgggctg
    32341 gcaacggtca attcgatatt tatattttgt gcatcgctgg cggcggccat attcattgag
    32401 cagacgtgga aaccacgatg acgcaccacg cgtaaaacac gttctaaggt ttctggattg
    32461 aagcgagccg atacattgac ctgatgttgc atcatgataa tttctccaac atttctgaat
    32521 tactggcgcc aggcggcacc agcggccaga cgttctcaag ttcgtcgatt gagacatgaa
    32581 gcaggtatgg cccatcactg ttcagcatgg tgtcgagtgc cgcttcaacc tggtctttac
    32641 gggtgatgtg ttggccaggg atgccgaagg cgctggctaa catgaggaaa tcggggttat
    32701 cagtaagggt ggtttcgctg tatcgttcct gaaaaaacag ttgctgccat tgtcgaacca
    32761 tccctaaccg ttggttatcg agtaagacga ttttcaacgg taactgcttg cgttttacgg
    32821 tgcccagctc ttgcacattc atcatgaaag agccgtcacc ggagatacag acaacggtat
    32881 cgttcggtcg cgcgacttgt gcgccaactg ccgccggtaa accaaaaccc atggtaccta
    32941 agccgctgga ggtgatgaaa ttttccgggc gagtgtgggc gatgtgctgc gcagcccaca
    33001 tctggtgctg ccccacatct gtggtcacga cgcaatccgc aggtttacga tccgacagtt
    33061 gttttaacaa caacggcgcg tagatagcgt caccgggatg gtcgtaacgc caggaatgtt
    33121 catcacgcag ctgcgcgcag tgttgctgcc agtcattgat atttaacggc tgctgtaatg
    33181 ctggtaacag agcatttaaa tcaccttgta atgccacatg tgcctgacgc agcttgttca
    33241 tttctgccgg gtcgatatcc atatggataa cactggcgtg tggcgcgaag gtgttcagtt
    33301 tgccggtcac ccggtcatca aaacgtgcgc ccacggcgat cagcaggtca cactcctgca
    33361 ccgcgaagtt tgccgctttg gtgccgtgca tccccagcat gcccagatag tacggataat
    33421 ctgcttctac tgcgcccagc cctttcagcg tacaggtggc aggcattttt gtggcagcga
    33481 gaaattcacg caaagccgga actgcctgcg ccatacccac gccaccgcca acgtacagca
    33541 tcggtttttg cgcttttgcc agcatctggc gcgcttgctc aacttcggca tgtgggaaag
    33601 tcacttcgtt ttcaacggtg gtgaaccacg gttccaggtc accgctggct aactggatat
    33661 cttttgggat atcgaccaga accggaccag gacgacctga gcaggcaacg tcgaatgctt
    33721 cagccatgat gcgcggcaac tcttccagcg actgcaccag aaagctgtgc ttggtacagg
    33781 ctaacgacaa tcccaggaca tccacttcct gaaatgcgtc agtgccgata aacggtgcgg
    33841 acacttgacc ggtgatggca acaacaggga tggaatctaa cagtgcgtcc gcaagcccgg
    33901 ttatcaggtt ggttgcgccc ggaccagacg tggcgataca tacgccagtt ttgccggtag
    33961 cacgagcata accgatagcc gccattgccg caccctgctc atgtcggcat agcaagtgct
    34021 ccacgccgcc gtcatacaat gcatcgtaaa ccggcataat tgcgccaccc ggataaccga
    34081 aaacggtgtt cacaccctgt gcccgcaacg catgtaccac ccactgtgcg ccattcatag
    34141 ttagttcccc gtcctgaatc ttgagaaaca gaattttgtg ctgttattca ttgtctgctc
    34201 ctcggttatg tttttaaggt caaaaaaaac ccccggacct ttcggtgcgg gggtcttagt
    34261 tcgttaaggc ttgatctcta agcctttcct cgtccaagtg cagccccgca cggtgggata
    34321 ataatcacca ccacgctaat cacgaccagg ctaatcactc gtagaagggc tgtcattttg
    34381 tcttttcttg catcttgttc gaaggaatgc ctaaagagtt accataggtt ttgtaaatag
    34441 t
//
LOCUS       DAJOSY010000038        34333 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJOSY010000038 DAJOSY010000000
VERSION     DAJOSY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.038"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(17..280)
                     /locus_tag="OHN27_001958"
     CDS             complement(17..280)
                     /locus_tag="OHN27_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2286401.1"
                     /translation="MIDLENQEREIINLMFSQGISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPSELLICPSWMTAEHK"
     gene            complement(312..1328)
                     /gene="lplA"
                     /locus_tag="OHN27_001959"
     CDS             complement(312..1328)
                     /gene="lplA"
                     /locus_tag="OHN27_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCQ2286402.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSTWIA
                     GAVR"
     gene            complement(1356..2000)
                     /locus_tag="OHN27_001960"
     CDS             complement(1356..2000)
                     /locus_tag="OHN27_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCQ2286403.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            2106..3074
                     /gene="serB"
                     /locus_tag="OHN27_001961"
     CDS             2106..3074
                     /gene="serB"
                     /locus_tag="OHN27_001961"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCQ2286404.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            3123..4505
                     /gene="radA"
                     /locus_tag="OHN27_001962"
     CDS             3123..4505
                     /gene="radA"
                     /locus_tag="OHN27_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCQ2286405.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            4526..5758
                     /gene="nadR"
                     /locus_tag="OHN27_001963"
     CDS             4526..5758
                     /gene="nadR"
                     /locus_tag="OHN27_001963"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCQ2286406.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            5792..5959
                     /locus_tag="OHN27_001964"
     CDS             5792..5959
                     /locus_tag="OHN27_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286407.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(6066..7733)
                     /gene="ettA"
                     /locus_tag="OHN27_001965"
     CDS             complement(6066..7733)
                     /gene="ettA"
                     /locus_tag="OHN27_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCQ2286408.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            8069..10006
                     /gene="sltY"
                     /locus_tag="OHN27_001966"
     CDS             8069..10006
                     /gene="sltY"
                     /locus_tag="OHN27_001966"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCQ2286409.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGSEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10096..10422
                     /gene="trpR"
                     /locus_tag="OHN27_001967"
     CDS             10096..10422
                     /gene="trpR"
                     /locus_tag="OHN27_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCQ2286410.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(10507..11028)
                     /gene="yjjX"
                     /locus_tag="OHN27_001968"
     CDS             complement(10507..11028)
                     /gene="yjjX"
                     /locus_tag="OHN27_001968"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCQ2286411.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            11080..11727
                     /gene="gpmB"
                     /locus_tag="OHN27_001969"
     CDS             11080..11727
                     /gene="gpmB"
                     /locus_tag="OHN27_001969"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCQ2286412.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(11724..12593)
                     /gene="robA"
                     /locus_tag="OHN27_001970"
     CDS             complement(11724..12593)
                     /gene="robA"
                     /locus_tag="OHN27_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCQ2286413.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGTYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            12804..13277
                     /gene="creA"
                     /locus_tag="OHN27_001971"
     CDS             12804..13277
                     /gene="creA"
                     /locus_tag="OHN27_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCQ2286414.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13290..13979
                     /gene="creB"
                     /locus_tag="OHN27_001972"
     CDS             13290..13979
                     /gene="creB"
                     /locus_tag="OHN27_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCQ2286415.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            13979..15403
                     /gene="creC"
                     /locus_tag="OHN27_001973"
     CDS             13979..15403
                     /gene="creC"
                     /locus_tag="OHN27_001973"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCQ2286416.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            15461..16814
                     /gene="creD"
                     /locus_tag="OHN27_001974"
                     /pseudo
     CDS             15461..16814
                     /gene="creD"
                     /locus_tag="OHN27_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
     gene            complement(16872..17588)
                     /gene="arcA"
                     /locus_tag="OHN27_001975"
     CDS             complement(16872..17588)
                     /gene="arcA"
                     /locus_tag="OHN27_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCQ2286417.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            17684..17824
                     /locus_tag="OHN27_001976"
     CDS             17684..17824
                     /locus_tag="OHN27_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286418.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            18224..18910
                     /locus_tag="OHN27_001977"
     CDS             18224..18910
                     /locus_tag="OHN27_001977"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCQ2286419.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            19124..19189
                     /gene="thrL"
                     /locus_tag="OHN27_001978"
     CDS             19124..19189
                     /gene="thrL"
                     /locus_tag="OHN27_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCQ2286420.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     misc_feature    19125..19244
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            19270..21732
                     /gene="thrA"
                     /locus_tag="OHN27_001979"
     CDS             19270..21732
                     /gene="thrA"
                     /locus_tag="OHN27_001979"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCQ2286421.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDELFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            21734..22666
                     /gene="thrB"
                     /locus_tag="OHN27_001980"
     CDS             21734..22666
                     /gene="thrB"
                     /locus_tag="OHN27_001980"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCQ2286422.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            22667..23953
                     /gene="thrC"
                     /locus_tag="OHN27_001981"
     CDS             22667..23953
                     /gene="thrC"
                     /locus_tag="OHN27_001981"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCQ2286423.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAIFGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            24167..24463
                     /locus_tag="OHN27_001982"
     CDS             24167..24463
                     /locus_tag="OHN27_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCQ2286424.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(24616..25392)
                     /gene="yaaA"
                     /locus_tag="OHN27_001983"
     CDS             complement(24616..25392)
                     /gene="yaaA"
                     /locus_tag="OHN27_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCQ2286425.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(25462..26892)
                     /locus_tag="OHN27_001984"
     CDS             complement(25462..26892)
                     /locus_tag="OHN27_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCQ2286426.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     IQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            27171..28124
                     /gene="tal"
                     /locus_tag="OHN27_001985"
     CDS             27171..28124
                     /gene="tal"
                     /locus_tag="OHN27_001985"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCQ2286427.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            28239..28826
                     /gene="mog"
                     /locus_tag="OHN27_001986"
     CDS             28239..28826
                     /gene="mog"
                     /locus_tag="OHN27_001986"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCQ2286428.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(28861..29427)
                     /gene="satP"
                     /locus_tag="OHN27_001987"
     CDS             complement(28861..29427)
                     /gene="satP"
                     /locus_tag="OHN27_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCQ2286429.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAEFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(29576..30289)
                     /gene="msyB"
                     /locus_tag="OHN27_001988"
     CDS             complement(29576..30289)
                     /gene="msyB"
                     /locus_tag="OHN27_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCQ2286430.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTCKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(30315..30719)
                     /locus_tag="OHN27_001989"
     CDS             complement(30315..30719)
                     /locus_tag="OHN27_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCQ2286431.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            31096..33027
                     /gene="dnaK"
                     /locus_tag="OHN27_001990"
     CDS             31096..33027
                     /gene="dnaK"
                     /locus_tag="OHN27_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCQ2286432.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQHAQQQTAGADASAN
                     NAKDDDVVDAEFEEVKDKK"
     gene            33116..34246
                     /gene="dnaJ"
                     /locus_tag="OHN27_001991"
     CDS             33116..34246
                     /gene="dnaJ"
                     /locus_tag="OHN27_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCQ2286433.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
BASE COUNT         8332 a         8579 c         9344 g         8078 t
ORIGIN      
        1 ctattcacga atctattcat ttatgttcag ccgtcatcca ggaagggcat atcagcaact
       61 ctgatggaca accataaatt tctgccattt tgcttttgat gtttgagcta agccgttccg
      121 tcttttcgat ctgttttaga gaattaatcg agatacctaa cattttactt acttcagcaa
      181 gcgataattt atgcctgata cgcacggcgg ctagccagga tataccctgg ctgaacatta
      241 aattgataat ctcccgttcc tggttctcca gatcgatcat atttccttcc ttggatattt
      301 taatcagcga gttaccgcac cgcccccgct atccacgtcg acaactcccg tagctctttt
      361 tcctgttccg ggaagtcaac caacagcgct tcgcactctt gttgcagcat atctgcgcgg
      421 tacaggcagc cttgcagtcg tccggcgagg gcttccagcg gcgcggggtt gaggctatcg
      481 gtaaacacct gggcgcgggt gatatggcct ttttcaacgt cgaaatgcag ttccacgccg
      541 ccccaggtaa agcgttcatc cagcagatgc gagaatgccg gagcctgacc gaagttccat
      601 tcccagctac tctggcgggc aaaggtttcg gcgaagttcg gcaagtctgg cgttttgtcc
      661 ggggagatga tttccgcttc tacacgctcg ccataatgag cgaaaaaggc ctcggttatg
      721 gcctcgcaaa cctgctcatg ggtgatcccc ggcaacagct cggtgaggtt ggtcacgcgg
      781 gaacgtaccg acgtaatgcc tttcgccgcc agtttctttt tatccggatt gagatagttt
      841 gccaggcggc taaggtcggc attgagcagc aaggtgccgt ggtggaagcc acgatctttg
      901 gtttcgcgat aggccgatcc tgagactttg cggtcgcctt cggcggtttt caccaccaga
      961 tcgttacgcc cggacgcttc ggcgctgacg ccaagcgcgt tcagcgcatt gagcacaatc
     1021 gacgtggaga tggttttatc gtactccggc ttgccagcca taaaggtaaa gcaggtattg
     1081 ccgagatcgt ggaacaccgc gccgccaccg ctactgcgcc gtgccaggcg gacgttatct
     1141 tcttccatcc gccgggtatt acactctttc cacgggttct gcgcgcgacc aatcactacc
     1201 gtgtcggcat tgcgccagag aaacagaacg cgctgcgtgg cgggcatctg gcgaaaaata
     1261 cactcctcca ccgccaggtt aaaccacggg tcgtaagagt cagagatgag caggcgtaat
     1321 gtggacataa cgatttcctt tcttgtagta atgggtcact cttttttctc gctttcttcc
     1381 tcttccggca ccggtttgct ggcggttaac aggaagggcg attgctgcca gcgggtacgt
     1441 ttaccctgta gcagcgtgcg ggtcagcact acgccgattg ccagtgaaag cagcaacatc
     1501 aggcgtaaaa tgttagtggt gttatccacc tgttgggctt cggtggcgag cgtatgggtg
     1561 tcgagcgtca ggcgcagata gccgagcggt ccgtttttcc cggcaattgg ttcgacaatc
     1621 tgctggttaa aatagccgcc cgctttttta ccgtcgagcg ccagccggtc gcgcacttcg
     1681 acgctttcgc cagaacgtgc gataagatcg ccttgttcgt catacacgcc cgcgtcgagg
     1741 atacggcttt catccgttaa ctgatcaagg atcgcctgaa tgcgtttttc atccggtgag
     1801 tcggtacgca tcagcggtgc aacgttcagc gttacctgac gcgccagggt gcgggccagt
     1861 tcttccagct gtggattacg ttgtcgctgg tggttttgac taaaccatga cgctccctgc
     1921 atcagcgcca ctaacaaggc aagacagaac aggacaatca ctgcccgatg cagccggaat
     1981 ttcagttttg tgcgagccat cttccaccct ttgaaaattt gagacttaat gttgccagaa
     2041 gcaatggata caaggtagcc tcatgcgtta ttttccctgc ttcgaacgat tttacaggag
     2101 ccttaatgcc taacattacc tggtgcgacc tgcctgaaga tgtctcttta tggccgggtc
     2161 tgcctctttc attaagtggt gatgaagtga tgccactgga ttaccacgca ggtcgtagcg
     2221 gctggctgct gtatggtcgt gggctggata aacaacgcct gacccaatac cagagcaaac
     2281 tgggcgcggc gatggtgatt gttgccgcct ggtgcgtgga agattatcag gtgattcgtc
     2341 tggcaggttc actcaccgcg cgcgctacgc gcctggccca tgaagcgcaa ctggatgtcg
     2401 cgccgctggg gaaaatcccg cacctgcgca cgccgggttt gctggtgatg gacatggatt
     2461 ccaccgccat ccagattgaa tgtattgatg aaattgccaa actggccgga acgggcgaga
     2521 tggtggcgga ggtaaccgaa cgggcgatgc gcggcgaact cgattttacc gccagcctgc
     2581 gcagccgcgt ggcgacgctg aaaggcgctg atgccaatat tctgcaacag gtgcgtgaaa
     2641 atctgccgct gatgccaggc ttaacgcagc tggtgctcaa gctggaaacg ctgggctgga
     2701 aagtggcgat tgcctccggc ggctttactt tctttgctga atacctgcgc gacaagctgc
     2761 gcctgaccgc cgtggtagcc aatgaactgg agatcatgga cggtaaattt accggcaatg
     2821 tgatcggcga catcgttgac gcgcagtaca aagcgaaaac tctgacccgc ctcgcgcagg
     2881 agtatgaaat cccgctggcg cagaccgtgg cgattggcga tggagccaat gacctgccga
     2941 tgatcaaagc ggcagggctg gggattgcct accatgccaa gccaaaagtg aatgaaaaga
     3001 cggaagtcac catccgtcac gctgacctga tgggggtatt ctgcatcctc tctggcagcc
     3061 tgaatcagaa gtaattgcct gcccgtcatc ctgcgggcgg cacagcatta acgaggtaca
     3121 ccgtggcaaa agctccaaaa cgcgcctttg tttgtaatga atgcggggcc gattatccgc
     3181 gctggcaggg gcagtgcagt gcctgtcatg cctggaacac catcaccgag gtgcgtcttg
     3241 ctgcgtcgcc aacggtggcg cgtaacgagc gtctcagtgg ctatgccggt agcgccgggg
     3301 tggcaaaagt ccagaaactc tccgacatca gccttgaaga gctgccgcgt ttttccaccg
     3361 gatttaaaga gttcgaccgc gtattaggcg gcggcgtggt accgggaagt gccattctga
     3421 ttggcggtaa cccaggcgcg gggaaatcca cgctgttgct gcaaacgctg tgcaaactgg
     3481 cccagcagat gaaaacgctg tatgtcaccg gcgaagagtc gctgcaacag gtggcaatgc
     3541 gcgctcatcg ccttggcctg ccgactgaca atctcaatat gttgtcggaa accagcatcg
     3601 agcagatctg cctgattgcc gaagaagagc aaccgaagct gatggtaatt gactccatcc
     3661 aggtgatgca tatggcggat gtacagtcat cgcctggtag cgtggcgcag gtgcgtgaaa
     3721 cggcggctta tttgacacgc ttcgccaaaa cgcgcggtgt ggcgattgtc atggtggggc
     3781 acgtaaccaa agatggctcg ctggctggcc cgaaagtgct ggaacactgt atcgactgtt
     3841 cggtgcttct ggatggcgat gccgactccc gttttcgcac cttacgcagc cataaaaacc
     3901 gcttcggcgc ggtgaatgag ctgggcgtct tcgcgatgac cgaacagggg ctgcgtgaag
     3961 tcagcaaccc ttccgccatc tttttaagcc gtggcgatga agtgacctct ggtagttcag
     4021 tgatggtggt atgggaagga acacgtccgt tgctggtgga gatccaggcg ctggtcgatc
     4081 actcgatgat ggcgaatccg cgccgcgtgg cggtaggtct ggagcaaaac cgtctggcga
     4141 tcctgctggc tgtcttgcac cgtcacggtg gtctgcaaat ggccgatcag gatgtgtttg
     4201 tgaacgtggt cggcggcgtg aaggtaacgg aaaccagcgc cgacttagcg ttactgctgg
     4261 cgatggtttc cagcctgcgt gacagaccgc tgccacagga tctggtggtg tttggtgaag
     4321 tcgggctggc aggggagatc cgcccggtgc ccagcggtca ggaacgaatt tcagaagcgg
     4381 cgaaacatgg ttttcgccgg gcgattgttc cggcggctaa cgtgccgaaa aaagcgccgg
     4441 aagggatgca gatttttggc gttaaaaaac tctccgacgc gcttagcgtg ttcgacgact
     4501 tataatgaga gatacggagg gagatatgtc gtcatttgat tacctgaaaa ctgccatcaa
     4561 gcaacagggc tgcacgctac agcaggtagc tgatgccagc ggtatgacca aagggtattt
     4621 aagccagtta ctgaatgcca aaatcaaaag ccctagcgcg caaaagctgg aggcgttgca
     4681 ccgttttttg gggcttgaat ttccccgaca gaagaaaacg atcggtgtgg tattcggtaa
     4741 gttctacccg ctgcataccg gacatatcta tcttatccag cgcgcctgta gccaggttga
     4801 cgagctgcat atcattatgg gttttgacga tacccgtgac cgcgcgttgt tcgaagacag
     4861 tgccatgtcg cagcagccga ccgtgccgga tcgtctgcgc tggttattgc aaacctttaa
     4921 atatcagaaa aatattcgca ttcatgcttt taacgaagag ggcatggagc cgtatccgca
     4981 cggctgggat gtgtggagca acggcatcaa aaagtttatg gctgaaaagg ggatccagcc
     5041 ggacctgatc tacacctcgg aagaagccga tgcgccacag tatatggaac atctggggat
     5101 cgagacggtg ctggtcgatc cgaagcgtac ctttatgagt atcagcggtg cgcagatccg
     5161 cgaaaacccg ttccgctact gggaatatat tcctacggaa gtgaagccgt tctttgtgcg
     5221 taccgtggcg atccttggcg gcgagtcgag cggtaaatcc accctggtaa acaaacttgc
     5281 caatatcttc aataccacca gtgcgtggga atatggtcgt gattatgtct tttcgcacct
     5341 cggtggtgat gagatcgcat tgcagtattc cgactacgat aaaatcgcgc tgggccacgc
     5401 tcaatacatt gattttgcag tgaaatacgc caataaagtg gcgtttattg acactgattt
     5461 tgtcactacc caggcgttct gcaaaaagta cgaagggcgt gagcatccgt tcgtgcaggc
     5521 gttgattgat gaataccgtt tcgatctggt gatcctgctg gagaacaaca cgccgtgggt
     5581 ggcggatggt ttacgcagcc tcggcagttc ggtggatcgc aaagagttcc agaacttgct
     5641 ggtggagatg ctggaagaga acaatatcga attcgtgcgg gttgaagagg acgattacga
     5701 cagccgtttc ctgcgctgcg tggagctggt gcgggagatg atgggggagc aaggataacc
     5761 gtgacgaaac tgctcaaagg cgaggtataa aatgagtttt tttgatgagt tgaaaacctc
     5821 tctggaagag gctgtcgaga ttaaacaagg tttgaaaaaa cctgcacggg tgacccgcca
     5881 cgaaattgag gatgctaagg ctgttgtaga ccggaaaagg tgttcacgcc gcatccggca
     5941 ttcggtgctc aatgcctgat gcgacgctta ccgcgtctta tcaggcctac agttttacaa
     6001 cgtattgaaa ttgctggttt tgtaggccgg ataaggcgtt cgcgccgcat ccggcatttt
     6061 acgcattact tcgcaatacg cttgtacttg atacgcttcg gctccagcgc gtctgcgcca
     6121 agcgtgcgtt ttttgtactc ttcgtattcg gtaaagttac cttcgaagaa ctcaacttta
     6181 ccttcatcct ggtagtccag gatgtgcgtg gcgatacggt cgaggaacca acggtcgtgc
     6241 gagataacca tcgcgcagcc cgggaactcc agcagggcgt tttccagcgc gcgcagggtt
     6301 tcgatatcca ggtcgttggt tggttcgtcg agcagcagca tgttgccgcc aacctgcagc
     6361 agcttcgcca gatgcagacg accgcgctca ccaccggaaa gttcaccaac gcgtttaccc
     6421 tgatcaaccc ctttaaagtt aaagcggcca acgtaggcgc ggcttggcat ctcggtgttg
     6481 ccgatcttca taatatccag cccgccggaa acttcttccc aaacggtttt gctgttatcc
     6541 attgagtcac ggaactgatc aaccgatgcc agtttcaccg tttcacccaa agtgatggtg
     6601 ccgctgtccg gctgttcctg accggagatc atacggaaca gggtcgattt acccgcgccg
     6661 ttcggaccga tgatcccgac gatcgcccct ttcgggatcg agaagctcag gtcatcaatc
     6721 agcaggcgat cgccgtagga tttacgcagg ttgctgactt ccagcacttt atcgcccaga
     6781 cgcggtccag gtggaataaa cagttcgttg gtttcgttac gtttctgata ttcggtgctg
     6841 ttcagctctt caaagcgcgc cagacgtgct ttacctttcg actggcggcc tttagttccc
     6901 tgacgtaccc actccagctc tttctcgatc gacttacgac gcgccgcttc ttgtgaagct
     6961 tcctgcgcca gacgctgatc tttctgctcc agccaggagg agtagttacc ttcccacgga
     7021 ataccttcac cgcggtccag ttcgaggatc cagcctgcaa cgttatcgag gaagtaacgg
     7081 tcgtgagtaa tcgccaccac ggtgccttcg aagtcgtgca ggaagcgttc cagccacgcc
     7141 acggattcgg catccaggtg gttggtcggt tcgtcgagca gcagcatgtc tggtttttcc
     7201 agcagcaggc ggcacaacgc cacgcgacga cgctcaccac cggagaggtt agcgattttc
     7261 gcgtcccaat ctggcagacg cagcgcatcc gccgcacgct ccagctgtac gttcaggtta
     7321 tgaccgtcgt gagcctgaat gatctcttcc agacggcctt gttcagcggc cagcttgtca
     7381 aaatcggcat ccggatcggc gtacagcgca tacacttcat ccaggcgttt cagggcgtta
     7441 accacttcag aaaccgcttc ttcaatggac tcacgcacgg tgtgttccgg gttcagctgc
     7501 ggttcctgcg gcagataacc aatcttgatg tctggctgcg gacgtgcttc accttcgatg
     7561 tctttatcaa tgcccgccat aatgcgcagc agggtagact tacccgcgcc gttcagaccc
     7621 aggacaccaa tttttgcccc agggaagaaa ctcagagaga tgtttttcaa aatatgacgt
     7681 ttcggcggaa caactttgcc gacacgatgc atggtataaa cgaattgagc cacgttggac
     7741 ttcgcctcta tgtttatcgt gatgatgagt tttcaaaggc gaagtgtagc ctgtttcctt
     7801 atctaatccc agccggtcga tcaccctcgc agtatactcg tcatacttca agttgcatgt
     7861 gctgcgctta cgctcgctca ccccagtcac ttacttctgt aagctcctgg ggactcactc
     7921 acttatcgcc ttcctgcaac tcgaattatt tagagtataa aagtaaaaaa gtgtccgtaa
     7981 cgtggcgtaa acggcaatga ctggttagca taaatctatt acgcggcatg acgctgcatt
     8041 gatgtattta cacttagagg atgcgcttgt ggaaaaagcc aaacaagtta cctggcggct
     8101 gttggctgcc ggtgtctgtc tgctgacggt cagcagcgtg gcgcgagccg actcactgga
     8161 tgagcagcgt agccgttacg cgcaaatcaa gcaggcctgg gataatcgac aaatggatgt
     8221 ggtcgaacaa atgatgcctg gactgaaaga ttatccactt tatccctacc tggaataccg
     8281 tcagatcacc gatgacctga tgaatcaacc ggcggtgacg gtcactaact ttgttcgcgc
     8341 taaccccacg cttcctcccg ctcgcacgct gcaatctcgt ttcgtcaatg aactggcgcg
     8401 gcgtgaagac tggcgtggct tgttagcctt tagcccggaa aagcccggaa ctaccgaagc
     8461 gcaatgtaat tactactatg caaaatggaa caccgggcag agtgaagaag cctggcaagg
     8521 ggcgaaagag ctgtggctaa ccggcaagag ccagcctaac gcctgtgaca agttatttag
     8581 tgtctggcgt gcgtcaggta aacaagatcc gctggcgtat ttagagcgta tccgtctggc
     8641 gatgaaagcg ggtaacactg gcctggtaac agtgctggca gggcagatgc ctgccgatta
     8701 ccagactatc gcctcggcaa tcatttcact ggcgaacaac cctaatacgg tactgacctt
     8761 cgcgcgtaca accggagcga ccgattttac ccgtcaaatg gcggcggtgg cgtttgccag
     8821 tgtggcgcgg caggatgctg aaaatgcacg gctgatgatc ccatcgcttg cccaggcgca
     8881 gcagcttaat gaagatcaga ttcaggagct gcgcgatatc gtcgcctggc gtttgatggg
     8941 caacgatgtc accgacgagc aggcgaaatg gcgcgatgac gccattatgc gctcgcaatc
     9001 tacttcgctt attgaacgcc gtgtacgaat ggcgcttggc accggcgatc gtcgcggcct
     9061 gaatacctgg ctggcgcgtt tgccgatgga ggcgaaagag aaagatgaat ggcgttactg
     9121 gcaggcggat ttactgctgg aacgcggacg tgaagctgaa gcaaaagaga ttttgcatca
     9181 actcatgcaa cagcgtggtt tctacccgat ggttgctgca caacgcatcg gcgaagagta
     9241 tgagctgaag attgataaag cgccgcagaa tgttgacagc gccctgactc aggggtcgga
     9301 gatggcgcgc gtgcgcgagt tgatgtactg gaatctcgat aacaccgcgc gtagcgagtg
     9361 ggccaatctg gtgaagagca agtcaaaaac agagcaggct caactggcgc ggtatgcttt
     9421 caacaaccaa tggtgggatc ttagcgttca ggcaacgatc gccgggaagc tgtgggatca
     9481 tctggaagag cgattcccgc tggcttacaa cgatcttttc aaacgttaca ccagtgggaa
     9541 ggagatcccg caaagctatg cgatggcgat tgcccgtcag gagagcgcct ggaatccaaa
     9601 agtgaaatca ccggtagggg ccagcggcct gatgcagatt atgcctggta cagcgaccca
     9661 tacggtgaag atgttctcta ttcctggtta tagcagccct gggcaattgc tggatccgga
     9721 aacgaatatc aacattggca ccagttatct gcaatatgtt tatcagcagt ttggcaataa
     9781 ccgtattttc tcctcagcag cttataacgc cggaccaggg cgggtgcgaa cctggcttgg
     9841 caacagcgcc gggcgtatcg acgcagtggc atttgtcgag agtattccgt tctccgagac
     9901 gcgtggttat gtaaagaacg tgctggctta tgacgcttac taccgctatt tcatggggga
     9961 taaaccgacg ttgatgagcg ccacggaatg gggacgtcgt tactgatccg cccgtttatg
    10021 atatgctatc gtactcttta gcgagtacaa ccgggggagg cattttgctt cccccgctaa
    10081 caatggcgac atattatggc ccaacaatca ccctattcag cagcgatggc agaacagcgt
    10141 caccaggagt ggttacgttt tgtcgacctg cttaagaatg cctaccaaaa cgatctccat
    10201 ttaccgttgt taaacctgat gctgacgcca gatgagcgcg aagcgttggg gactcgcgtg
    10261 cgtattgtcg aagagctgtt gcgcggcgaa atgagccagc gtgagttaaa aaatgaactc
    10321 ggcgcgggca tcgcgacgat tacgcgtgga tctaacagcc tgaaagccgc gcccgttgag
    10381 ctgcgccagt ggctggaaga ggtgttgctg aaaagcgatt gattttgtag gcctgataag
    10441 acgcggcagc gtcgcatcag gcatcgtgca ccgaatgccg gatgcggcgt gaacgcctta
    10501 tccgtcctac aatgcctgat atacggcatt gtgaaacgga ctcagcgcca ggatcaccgc
    10561 ctggtgatag acgctggcgc gagtgagttt cccggcggta aacacgccga tcgccccttc
    10621 cttacgacca atctcatcaa taccggtata acgcgacatc acgggaccaa gcgcctcgcc
    10681 ttcacgcact ttttccagaa tcaccgcagg caacggcaaa gtagccgaac gcgcctcgcc
    10741 gcgctggctg gcgttttcaa tcaccaccca actgaaagtg ctgtcaccat cgatgccagc
    10801 ttcaatcgcc acccaaaaat cagcctctgg aagtaaacgg cgggcatttg ctacccgatt
    10861 tcgtgcgcca gcgcgcgttt cctcactgcc aaagggttgt tccggtacac cgctctcgac
    10921 ggcaacggat gcaatatggc aggatccttc gccgaagatc tcgtgaaatg cctgcagaat
    10981 ggcctgaatt ttagcgggat tggtggtcgc acagacaact tggtgcataa tcagcattac
    11041 tcagaaaatt aacgttacag cagtatacgg aaaaaaagca tgttacaggt atacctagtc
    11101 cgccacggtg aaacgcagtg gaacgccgag cgacgtattc agggccagtc tgacagcccg
    11161 ctgaccgcca aaggtgagca acaggcgatg caggtggcaa cccgtgccaa agagcttggc
    11221 attacgcata ttatcagtag cgatttagga cgcacccggc gtacggcgga aatcatcgcc
    11281 caggcctgcg gctgtgacat catctttgat tctcgcctgc gtgaattaaa catgggtgtg
    11341 ctggaaaaaa gacatatcga ttctctgacc gaagaagaag agaactggcg tcggcagctg
    11401 gtcaatggca ccgttgacgg gcgtattcct gaaggcgagt caatgcagga gctcagcgat
    11461 cgcgtcaatg cggcactgga atcttgccgg gacttaccgc agggaagccg accgctgctg
    11521 gtgagccatg gtattgcact gggatgtctg gtgagtacga ttctcggatt accagcatgg
    11581 gcagagcgcc gcttacgtct gcgtaactgt tctatttcgc gcgtggatta tcaggaaagc
    11641 ctgtggctgg cgtctggctg ggtcgttgaa accgcagggg acatctcgca tctggacgcc
    11701 cctgcattag atgagctgca gcgttaacgg cggataggaa tcagcagttc acagcgcaga
    11761 ttaattgggc gatctcccgc tttggcgtct tctgccgggt agtatcgctc aatatcctga
    11821 cctttacggc gcgtcagatt gagcattggc atgcacgttc cgtataccgt caggataaac
    11881 tcctgcacgc cggttcccag accttcatag gtaaacatca catattcgcc gccctgcagc
    11941 atcaccggat gccccgtcag tacatagcca tctgcctgat cctgggctaa cgcggtggta
    12001 tagaatacct cttgttcgtc gtctttatcc tgactcggac gcgtttcatt caggccgtag
    12061 agcaccggcg gaatggtcgg cgcgttgccg agaaaatcgt gccagaactg ataacgcatt
    12121 tcatggcgga aatcagagat ttgctccagc gaacaggagt agctctgggt aacaccaatc
    12181 agcggcgtat cttccagggt gacaaatttg tgctctggca ttgtgaattc acccagacgt
    12241 agcggcgggc gaataccaaa ggcgctccat tcaggagaac ggcggtaaag tgcaggagtc
    12301 tgggcaaact gcttcttgaa tgcgcgggta aatgtctgtt gagagtcgaa gcggtattgc
    12361 agcgcgatgt ccagaatcgg acgcgcagtc aggcgtagtg cgaccgccga tttcgacaaa
    12421 cgacgagcac gaatatacgt gccaatagca tggccagtga catctttaaa cattctctgt
    12481 aagtgccact tggaataacc tgctttcgcc gctacattgt cgagcgacag gggctgatcc
    12541 agatgacctt ccagccagat taaaaggtcg cgaataatgc cggcctgatc cataaaatat
    12601 cctcatcctt tcaacaacga gcacctgaca tcaggtaatt ggataatagc atttcttgct
    12661 gttttagcat tcagtgtttt tttcttagta gagtatgttt tagggcttga cggaaataaa
    12721 agtattgaga ttttgttctt aatcaatatg ttatttaccg tgacgaacta attgctcgtg
    12781 taatagataa aaatggtaac aatatgaaat acaagcattt gatcctgtct ttaagcctga
    12841 taatgctggg gccattggct catgcagaag agattggttc ggtcgacacc gtatttaaaa
    12901 tgatcggacc ggatcacaaa attgttgtgg aagcctttga cgatcccgat gtgaaaaatg
    12961 tcacctgtta tgtgagccgg gcgaaaaccg ggggtattaa agggggattg ggcctggcag
    13021 aagatacctc cgatgcggcc atttcttgtc agcaagtcgg gccgattgaa ttgtcggatc
    13081 gtattaaaaa cggcaaagct cagggcgagg tagtattcaa aaaacgcacg tccctggtct
    13141 ttaagtcgtt acaggtcgtg cgcttttatg atgccaaacg caacgcgctc gcttatctgg
    13201 cttactccga caaagttgta gaaggttcgc cgaaaaacgc gattagcgcg gttcctgtca
    13261 tgccgtggcg gcaataacag aggcgattta tgcaacggga aacggtctgg ttagtggaag
    13321 atgagcaagg gatagccgac acgctggtct acatgttgca gcaggaaggt tttgccgtcg
    13381 aggtctttga gcgaggcttg ccggtgctgg ataaagctcg ccagcaggct cccgacgtca
    13441 tgattctcga tgttggtttg cctgatatca gcggctttga attatgccgc cagttactgg
    13501 cgctccatcc ggcgttacct gtactgttcc tgacggcccg aagtgaagag gtcgatcgcc
    13561 tgcttgggct ggaaattggc gctgacgact atgtggctaa accgttttca ccccgcgaag
    13621 tgtgcgccag ggtgcgcacc ttactgcgtc gggtgaagaa gttctcgtcg ccgtctcccg
    13681 tcatccgtat tggtcatttt gaattgaatg aacccgcggc gcagatcagc tggtttgaca
    13741 cgccattaac gctgacgcgg tatgagtttt tattgctgaa gacgttactc aagtcgccag
    13801 gccgcgtctg gtcccgccag caactaatgg atagcgtgtg ggaagatgcg caggacacct
    13861 acgatcgcac cgttgatacc cacattaaaa cgttgcgtgc caagctgcgc gccatcaacc
    13921 ccgatctttc accgattaat actcatcgcg gcatgggata tagcctgagg ggcctgtaat
    13981 gcgtatcggc atgcggttgc tgctgggcta ttttttactg gtggcggtgg cggcctggtt
    14041 cgtactggct atttttgtca aagaagttaa accgggcgtg cgaagagcaa cggaggggac
    14101 gttgatcgac accgcaacgt tgctggcgga gctggcgcgt cccgatttgc tctctgggga
    14161 cccaacgcat gggcaactgg cgcaggcgtt taatcagcta caacatcgcc cgtttcgcgc
    14221 caatatcggt ggcattaaca aagtgcgcaa tgaatatcat gtctatatga ccgattcgca
    14281 gggtaaagta ttgttcgatt cggcaaataa agccgttggg caggattatt cgcgctggaa
    14341 tgacgtctgg ctaacgttgc gtggtcagta tggtgcgcgc agcacgttgc aaaatcctgc
    14401 cgatcccgaa agttcggtga tgtatgttgc cgcgccgatt atggacggct cgcggcttat
    14461 tggcgttttg agcgtaggca aaccgaacgc ggcgatggct ccggtcatta agcgtagcga
    14521 gcggcgaatt ttatgggcca gcgccatttt gctggggatt gcactggtga ttggcgcagg
    14581 catggtttgg tggatcaacc gctccattgc ccggctcact cgctatgccg attccgtcac
    14641 tgacaataag cccgttcctc ttcctgaact cggcagtagc gagctgcgta aactcgcgca
    14701 ggcgctggaa agtatgcgcg tgaagctgga agggaaaaac tatattgagc agtatgttta
    14761 tgcgttaacc catgagctaa aaagcccact ggcggcgatt cgtggcgcgg cggaaatttt
    14821 acgcgaaggt ccgccaccgg aagtggtggc tcgttttacc gacaacattc tgacgcaaaa
    14881 tgcgcgtatg caggcattgg tagaaacgtt actacgccag gcaagactgg agaatcgtca
    14941 ggaagtcgtt ctgactgctg ttgatgtggc ggcattattt cgccgcgtca gcgaagcgcg
    15001 caccgtgcag ttggcagaaa aaaacatcac tttgcatgtt atgcctactg aggttaacgt
    15061 tgctgctgaa ccggcgttac tggagcaggc gctggggaat ttactggata acgccatcga
    15121 ttttactccc gagagcggtt gcataacgct aagcgccgaa gtggatcagg aacacgtcac
    15181 gcttaaggtg ctggataccg gtagtggtat tcctgactac gcgctgtcac gtatttttga
    15241 acgcttttac tctttgccgc gtgcaaatgg gcaaaaaagc agcggtctgg ggttggcgtt
    15301 cgtcagtgag gtcgcccgtt tgtttaacgg cgaagtcacg ctgcgcaacg tgcaggaagg
    15361 tggcgtgctg gcctcgctac gacttcaccg tcacttcaca tagcttcaaa ttcttcccac
    15421 atagccttcg tatcctgctg ccattgcaaa ggagaagact atgttgaaat cccccctgtt
    15481 ctggaaaatg accaccctgt ttggtgcagt attgctgttg ttgattccga taatgctgat
    15541 tcggcaggtg attgtcgaac gtgccgatta ccgtagcgat gtggaagatg cgattcggca
    15601 aagtaccagc gggccgcaaa aactcgttgg gccgctcatc gctattcctg tgaccgagct
    15661 ttatacggtg caggaagacg ataaaaccgt ggagcggaaa cgaagtttta tccatttttg
    15721 gttacctgag tcattgatgg ttgatggtaa tcagaacgtg gaagaacgca agatagggat
    15781 ttataccggt caggtctggc acagtgattt aacgctaaaa gccgatttcg atgtttcgcg
    15841 tcttagcgaa ctcgacgcgc caaatatcac cttaggcaag ccatttattg tgattagcgt
    15901 cggggatgcg cgtggtattg gtgtggtgaa agcgcctgaa gttaacggaa cggcgctgac
    15961 cattgaaccc ggcaccgggt tagagcaagg cgggcagggc gtgcatatcc ctttacccga
    16021 aggggactgg cggaagcaga acctgaagct gaatatggcc ctgaatttaa gcggtaccgg
    16081 cgatctttct gtggtgcctg ccgggcgtaa tagcgaaatg accttaacca gcaactggcc
    16141 gcatcccagt tttttaggtg attttttgcc agccaaacgg gaagttagcg agtcaggttt
    16201 tcaggcgcaa tggcaaagca gctggtttgc taataatctc ggtgagcgtt ttgcttcagg
    16261 caatgatacc ggctgggaaa acttcccccg cgtttagcgt cgcagtgacg acgccagccg
    16321 atcaatacca attaactgac cgggcgacta agtacgctat tctgctgatt gcgctgactt
    16381 ttatggcgtt ctttgttttt gaaacgctca ccgcgcaacg tttacaccca atgcaatatt
    16441 tactggtggg gctttcatta gtgatgtttt atttgctctt gctggcgctt tctgaacata
    16501 ccggttttac cggggcatgg ataatcgcca gtctgattgg ggcgttaatg aacggtattt
    16561 atttgcaggc ggtattgaaa ggctggcgca acagcatgtt gtttaccctc gcgctgttgt
    16621 tgctggatgg tgtgatgtgg ggactactca actctgccga tagcgcgctg ttgttgggaa
    16681 ccagtgtgct ggtggtggcg ctggccggaa tgatgtttgt gacccgtaat atcgactggt
    16741 atgcgttttc actgccgaaa atgaaagcca ataaagaagt tacaacggac gatgaattac
    16801 gtatctggaa ataaggttga aaaaaaaacg gcgctaaaaa gcgccatttt ttttgacggt
    16861 ggtaacgccg attaatcttc cagatcaccg cagaagcgat aaccttcacc gtgaatggtg
    16921 gcgatgattt ccggcgtatc cggcgtagat tcgaaatgtt tacgaatacg gcggatcgtc
    16981 acgtctacag tacggtcgtg cggtttcagc tcacggccgg tcattttctt cagcagttca
    17041 gcacgagact gaattttgcc tgggttttca cagaagtgaa gcatggcgcg gaactcgctg
    17101 cgcggcagct tgtactgctc gccatcaggg ccgatcaacg aacggctgtt gatgtccagt
    17161 tcccaaccat tgaacttgta gctttcaacg ctacgacgtt cttcgctgac agtacccaga
    17221 ttcatggtac gggacagcag gttgcgtgca cgaatcgtca gttcacgcgg gttgaacggt
    17281 ttggtgatgt agtcatctgc accgatttcg aggccgagaa ttttatcgac ttcgttgtca
    17341 cggccagtca ggaacatcaa cgcaacattc gcctgctcgc gcagttcacg cgctaacaga
    17401 agaccgttct tacccggcag attgatatcc atgatcacca ggttgatgtc atattcagag
    17461 aggatctgat gcatttccgc gccatctgtc gcttcgaaaa catcatagcc ttccgcttcg
    17521 aaaatacttt tcaacgtgtt gcgtgttacc aactcgtctt caacgataag aatgtgcggg
    17581 gtctgcatgt ttgctaccta aattgccaac taaatcgaaa caggaagtac aaaagtccct
    17641 gacctgcctg atgcatgctg caaattaaca tgatcggcgt aacatgacta aagtacgtaa
    17701 ttgcgttctt gatgcacttt ccatcaacgt caacaacatc attagcttgg tcgtgggtac
    17761 tttccctcag gacccgacag tgtcaaaaac ggctgtcatc ctaaccattt taacagcaac
    17821 ataacaggct aagaggggcc ggacacccaa taaaactacg cttcgttgac atatatcaag
    17881 ttcaattgta gcacgttaac agtttgatga aatcatcgta tctaaatgct agctttcgtc
    17941 acattatttt aataatccaa ctagttgcat catacaacta ataaacgtgg tgaatccaat
    18001 tgtcgagatt tattttttat aaaattatcc taagtaaaca gaaggatatg tagcattttt
    18061 taacaattca accgttagta cagtcaggaa atagtttagc cttttttaag ctaagtaaag
    18121 ggctttttct gcgacttacg ttaaggattt gtaaattcgc accgcgtaat aagttgacag
    18181 tgatcaccct gttcgcggtc atttggtcaa gaagagtggc aatatgcgta taacgattat
    18241 tctggtcgca cccgccagag cagaaaatat tggggcagcg gcgcgggcaa tgaaaacgat
    18301 ggggtttagc gaactgcgga ttgtcgatag tcaggcacat ctggagccag ccactcgctg
    18361 ggtcgcacat ggatctggtg atattattga taatattaaa gttttcccga cactggttga
    18421 atcgttacac gatgtcgatt tcactgtcgc caccactgcg cgcagtcggg cgaaatatca
    18481 ttactacgca acgccagtag aactggtgcc gctgttagag gaaaaatctt catggatgag
    18541 ccatgccgcg ctggtgtttg gtcgcgaaga ttccgggctg actaacgaag agttagcgtt
    18601 ggctgacgtt cttactggtg tgccgatggt ggcggattat ccttcgctca atctggggca
    18661 ggcagtgatg gtctattgct atcaattagc aacattaata caacaaccga cgaaaagtga
    18721 cacaacggca gaccaacatc aactgcaagc tttacgcgaa cgagtcatgg cattgctgac
    18781 gactctggca gtggcagatg acataaaact ggtcgactgg ttacagcaac gcctggggct
    18841 tttagagcaa cgagacacgg caatgttgca ccgtttgctg catgatattg aaaaaaatat
    18901 caccaaataa aaaacgcctt agtaagtatt tttcagcttt tcattctgac tgcaacgggc
    18961 aatatgtctc tgtgtggatt aaaaaaagag tgtctgatag cagcttctga actggttacc
    19021 tgccgtgagt aaattaaaat tttattgact taggtcacta aatactttaa ccaatatagg
    19081 catagcgcac agacagataa aaattacaga gtacacaaca tccatgaaac gcattagcac
    19141 caccattacc accaccatca ccattaccac aggtaacggt gcgggctgac gcgtacagga
    19201 aacacagaaa aaagcccgca cctgacagtg cgggcttttt tttcgaccaa aggtaacgag
    19261 gtaacaacca tgcgagtgtt gaagttcggc ggtacatcag tggcaaatgc agaacgtttt
    19321 ctgcgggttg ccgatattct ggaaagcaat gccaggcagg ggcaggtggc caccgtcctc
    19381 tctgcccccg ccaaaatcac caaccacctg gtggcgatga ttgaaaaaac cattagcggc
    19441 caggatgctt tacccaatat cagcgatgct gaacgtattt ttgccgaact tctgacggga
    19501 ctcgccgccg cccagccggg attcccgctg gcgcaattga aaactttcgt cgaccaggaa
    19561 tttgctcaaa taaaacatgt cctgcatggc attagtttgt tagggcagtg cccggatagc
    19621 atcaacgctg cgctgatttg ccgtggcgag aaaatgtcga tcgccattat ggccggcgtg
    19681 ttagaagcgc gtggtcacaa cgttaccgtt atcgatccgg tcgaaaaact actggcagtg
    19741 gggcattacc tcgaatctac cgtcgatatt gctgagtcca cccgccgtat tgcggcaagt
    19801 cgtattccgg ctgatcacat ggtgctgatg gcaggtttca ccgccggtaa tgaaaaaggc
    19861 gaactggtgg tgcttggacg taacggttcc gactactccg ctgcggtgct ggctgcctgt
    19921 ttacgcgccg attgttgcga gatttggacg gacgttgacg gggtttatac ctgcgacccg
    19981 cgtcaggtgc ccgatgcgag gttgttgaag tcgatgtcct accaggaagc gatggagctt
    20041 tcctacttcg gcgctaaagt tcttcacccc cgcaccatta cccccatcgc ccagttccag
    20101 atcccttgcc tgattaaaaa taccggaaat cctcaagcac caggtacgct cattggtgcc
    20161 agccgtgatg aagacgaatt accggtcaag ggcatttcca atctgaataa catggcaatg
    20221 ttcagcgttt ccggcccggg gatgaaagga atggtcggca tggcggcgcg cgtctttgct
    20281 gcaatgtcac gcgcccgtat ttccgtggtg ctgattacgc aatcatcttc cgaatacagt
    20341 atcagtttct gcgttccgca aagcgactgt gtgcgagctg aacgggcaat gcaggaagag
    20401 ttctacctgg aactgaaaga aggcttactg gagccgctgg cggtgacgga acggctggcc
    20461 attatctcgg tggtaggtga tggtatgcgc accttgcgtg ggatctcggc gaaattcttt
    20521 gccgcgctgg cccgcgccaa tatcaacatt gtcgccattg ctcagggatc ttctgaacgc
    20581 tcaatctctg tcgtggtaaa taacgatgat gcgaccactg gcgtgcgcgt tactcatcag
    20641 atgctgttca ataccgatca ggttatcgaa gtgtttgtga ttggcgtcgg tggcgttggc
    20701 ggtgcgctgc tggagcaact gaagcgtcaa caaagctggc tgaagaataa acatatcgac
    20761 ttacgtgtct gcggcgttgc caactcgaag gctctgctta ccaatgtgca tggcctaaac
    20821 ctggaaaact ggcaggaaga actggcgcaa gccaaagagc cgtttaatct cgggcgctta
    20881 attcgcctcg tgaaagaata tcatctgcta aacccggtca ttgttgactg cacctccagc
    20941 caggcagtgg cggatcaata tgccgacttc ttgcgcgaag gtttccacgt tgtcacgccg
    21001 aacaaaaagg ccaacacctc gtcgatggat tactaccatc tgttgcgtca tgcggcggaa
    21061 aaatcgcggc gtaaattcct ctatgacacc aacgttgggg ctggattacc ggttattgag
    21121 aacctgcaaa atctgctcaa tgctggtgat gaattgatga agttctccgg cattctttca
    21181 ggttcgcttt cttatatctt cggcaagtta gacgaaggca tgagtttctc cgaggcgact
    21241 actctggcgc gggaaatggg ttataccgaa ccggatccgc gagatgatct ttctggtatg
    21301 gatgtagcgc gtaagctatt gattctcgct cgtgaaacgg gacgtgaact ggagctggcg
    21361 gatattgaaa ttgaacctgt gctgcccgca gagtttaacg ccgagggtga tgttgccgct
    21421 tttatggcga atctgtcaca gctcgacgag ctctttgccg cgcgcgtggc gaaggcccgt
    21481 gatgaaggaa aagttttgcg ctatgttggc aatattgatg aagatggcgt ctgccgcgtg
    21541 aagattgccg aagtggatgg gaatgatccg ctgttcaaag tgaaaaatgg cgaaaacgcc
    21601 ctggccttct atagccacta ttatcagccg ctgccgttgg tgctgcgcgg atatggtgcg
    21661 ggcaatgacg ttaccgctgc tggtgtcttt gccgatctgc tacgtaccct ctcatggaag
    21721 ttaggagtct gacatggtta aagtttatgc cccggcttcc agtgccaata tgagcgtcgg
    21781 gtttgatgtg ctcggggcgg cggtgacacc cgttgatggt gcattgctcg gagatgtagt
    21841 cacggttgag gcggcagaga cattcagtct caacaacctc ggacgctttg ccgataagct
    21901 gccgtcagaa ccacgggaaa atatcgttta tcagtgctgg gagcgttttt gccaggagct
    21961 tggcaagcaa attccagtgg cgatgactct ggaaaagaat atgccgatcg gttcgggctt
    22021 aggctccagc gcctgttcgg tggtcgcggc gctgatggcg atgaatgaac actgcggcaa
    22081 gccgcttaat gacactcgtt tgctggcttt gatgggcgag ctggaaggac gtatctccgg
    22141 cagcattcat tacgacaacg tggcaccgtg ttttcttggt ggtatgcagt tgatgatcga
    22201 agaaaacgac atcatcagcc agcaagtgcc tgggtttgat gagtggctgt gggtgctggc
    22261 gtatccgggg attaaagtct cgacggcaga agcccgggct attttaccgg cgcagtatcg
    22321 ccgccaggat tgcattgcgc acgggcgaca tctggcaggc ttcattcacg cctgctattc
    22381 ccgtcagcct gagcttgccg cgaagctgat gaaagatgtt atcgctgaac cctaccgtga
    22441 acggttactg cctggcttcc ggcaggcgcg gcaggcggtc gcggaaatcg gcgcggtagc
    22501 gagcggtatc tccggctccg gcccgacctt gttcgctctg tgtgacaagc cggataccgc
    22561 ccagcgcgtt gccgactggt tgggtaagaa ctacctgcaa aatcaggaag gttttgttca
    22621 tatttgccgg ctggatacgg cgggcgcacg agtactggaa aactaaatga aactctacaa
    22681 tctgaaagat cacaatgagc aggtcagctt tgcgcaagcc gtaacccagg ggttgggcaa
    22741 aaatcagggg ctgttttttc cgcacgacct gccggaattc agcctgactg aaattgatga
    22801 gatgctgaag ctggattttg tcacccgcag tgcgaagatc ctctcggcgt ttattggtga
    22861 tgaaatcccg caggaaatcc tggaagagcg cgtgcgcgcg gcgtttgcct tcccggctcc
    22921 ggtcgccaat gttgaaagcg atgtcggttg tctggaattg ttccacgggc caacgctggc
    22981 atttaaagat ttcggcggtc gctttatggc acaaatgctg acccatattg cgggcgataa
    23041 gccagtgacc attctgaccg cgacctccgg tgataccgga gcggcagtgg ctcatgcttt
    23101 ctacggttta ccgaatgtga aagtggttat cctctatcca cgaggcaaaa tcagtccact
    23161 gcaagaaaaa ctgttctgta cattgggcgg caatatcgaa actgttgcca tcgacggcga
    23221 tttcgatgcc tgtcaggcgc tggtgaagca ggcgtttgat gatgaagagc tgaaagtggc
    23281 gctggggtta aactcagcta actcgattaa catcagccgt ttgctggcgc agatttgcta
    23341 ctactttgaa gcagttgcgc aactgccgca ggaagcgcgc aaccagctgg ttgtctcggt
    23401 gccaagcgga aacttcggcg atttgacggc gggtctgctg gcgaagtcac tcggtctgcc
    23461 ggtgaaacgt tttattgctg cgaccaacgt gaacgatacc gtgccacgtt tcctgcacga
    23521 cggtcagtgg tcacccaaag cgactcaggc gacgttatcc aacgcgatgg acgtgagtca
    23581 gccgaacaac tggccgcgtg tggaagagtt gttccgccgc aaaatctggc aactgaaaga
    23641 gctgggttat gcagccgtgg atgatgaaac cacgcaacag acaatgcgtg agttaaaaga
    23701 actgggctac acctcggagc cgcacgctgc cgtagcgtat cgtgcgctgc gtgaccagtt
    23761 gaatccaggc gaatatggct tgttcctcgg caccgcgcat ccggcgaaat ttaaagagag
    23821 cgtggaagcg attttcggtg aaacgttgga tctgccaaaa gagctggcag aacgtgctga
    23881 tttacccttg ctttcacata atctgcccgc cgattttgct gcgttgcgta aattgatgat
    23941 gaatcatcag taaaatctat tcattatctc aatcaggccg ggtttgcttt tatgcagccc
    24001 ggctttttta tgaagaaatt atggagaaaa acgacaggga aaaaggagaa attctcaata
    24061 aatgcggtaa cttagagatt aggattgcgg agaataacaa ccgtcgttct catcgcgtaa
    24121 tctccggata tcgacccata acgggcaatg ataaaaggag taacctgtga aaaagatgca
    24181 atctatcgta ctcgcacttt ccctggttct ggtcgctccc atggcagcac aggctgcgga
    24241 aattacgtta gtcccgtcag taaaattaca gataggcgat cgtgataatc gtggctatta
    24301 ctgggatgga ggtcactggc gcgaccacgg ctggtggaaa caacattatg aatggcgagg
    24361 caatcgctgg cacccacacg gaccgccgcc accgccgcgc caccataaga aagctcctca
    24421 tgatcatcac ggcggtcatg gtccaggcaa acatcaccgc taaatgacaa atgccgggta
    24481 acaatccggc attcagcgcc tgatgcgacg ctggcgcgtc ttatcaggcc tacgtgaatt
    24541 ctgcaatata ttgaatctgc atgcttttgt aggccggata aggcgttcac gccgcatccg
    24601 gcattgactg caaacttaac gctgctcgta gcgtttaaac accagttcgc cattgctgga
    24661 ggaagcttca tcaaagaagt aaccttcgct attaaaacca gtcagttgct ctggtttggt
    24721 cagccgattt tcaataataa aacgactcat cagaccgcgt gctttcttag cgtagaagct
    24781 gattatctta aatttgccgt tcttctcatc gaggaacacc ggcttgataa tctcggcatt
    24841 caatttcttc ggcttcaccg atttaaaata ctcatctgac gccagattaa tcaccacatt
    24901 atcgccttgt gctgcgagcg cctcgttcag cttgttggtg atgatatctc cccagaattg
    24961 atacagatct ttccctcggg cattctcaag acggatcccc atttccagac gataaggctg
    25021 cattaaatcg agcgggcgca gtacgccata caagccggaa agcattcgca aatgctgttg
    25081 ggcaaaatcg aaatcgtctt cgctgaaggt ttcggcctgc aagccggtgt agacatcacc
    25141 tttaaacgcc agaatcgcct ggcgggcatt ctccggcgtg aaatctggct gccagtcatg
    25201 aaagcgagcg gcgttgatac ccgccagttt gtcgctgatg cgcatcagcg tgctaatctg
    25261 cggaggcgtc agtttccgcg cttcatggat caactgctgg gaattgtcta acagctccgg
    25321 cagcgtatag cgcgtggtgg tcaacgggct ttggtaatca agcgttttcg caggtgaaat
    25381 aagaatcagc atatccagtc cttgcaggaa atttatgccg actttagcaa aaaaagagaa
    25441 tgagttgatc gatagttgtg attactcctg cgaaacatca tcccacgcgt ccggagaaag
    25501 ctggcggccg atatccggat aacgcaacgg atcaaacacc gggcgcacgc cgagtttacg
    25561 ctggcgtaga taatcactgg caatggtatg aaccacaggc gagagcagta aaatggcggt
    25621 caaattggta atagccatgc aggccattat gatatctgcc agttgccaca tcagcggaag
    25681 acttagcaag gtgccgccga tgaccgttgc gaaggtgcag atccgcaaac accagatcgc
    25741 tttagggttg ttcaggcgta aaaagaagag attgttttcg gcgtaaatgt agttggcaac
    25801 gatggagctg aaggcaaaca gaataaccac gagggtaaca aactcagcac cccaggaacc
    25861 cattagcacc cgcatcgcct tctggataag ctgaatacct tccagcggca tgtaggttgt
    25921 gccgttaccc gccagtaata tcagcatggc gcttgccgta cagatgacca gggtgtcgat
    25981 aaaaatgcca atcatctgga caatcccttg cgctgccgga tgcggaggcc aggacgccgc
    26041 tgccgctgcc gcgtttggcg tcgaccccat tcccgcctca ttggaaaaca tactgcgctg
    26101 aaaaccgtta gtaatcgcct ggcttaaggt atatcccgcc gcgccgcctg ccgcttcctg
    26161 ccagccaaaa gcactctcaa aaatagacca aatgacgtgg ggaagttgcc cgatattcat
    26221 tacgcaaatc accaggctgg tcagtaccca gattatcgcc atcaacggga caaacccctg
    26281 catgagccgg gcgacgccat gaagaccgcg agtgatcgcc agcagagcaa agacagcgag
    26341 aataatacct gtcaccagcg ggggaaaatc aaaagaaaaa ctcagggctc gcgcaacggc
    26401 gttcgcctga attccgctga aaattatgcc gtaggcgatg agcaaaaaga cggcgaacag
    26461 aacgcccatc cagcgcatcc ccagcccgcg cgccatatac catgccggtc cgccacgaaa
    26521 ctgcccattg gcatcacgtt ctttataaag ctgtgcgagg gaacattcgg caaacgaggt
    26581 cgccatgccg ataaacgcgg caacccacat ccagaagacg gctccaggtc caccggcggt
    26641 aattgccagc gcaacgccgg ccaggttgcc gctacccacg cgcgccgcaa gactggtaca
    26701 caatgactga aatgaggtta aaccacctgg ctgtggatga atgctatttt taagactttt
    26761 gccaaactgg cggatgtagc gaaactgcac aaatccggtg cgaaaagtga accaacaacc
    26821 tgcgccgaag agcaggtaaa tcattaccga tccccaaagg acgctgttaa taaaggagaa
    26881 aaaatctggc atgcatatcc ctcttattgc cggtcgcgat gactttcctg tgtaaacgtt
    26941 accaattgtt taagaagtat atacgctacg aggtacttga taacttctgc gtagcataca
    27001 tgaggttttg tataaaaatg gcgggcgata tcaacgcagt gtcagaaatc cgaaacagtc
    27061 tcgcctggcg ataaccgtct tgtcggcggt tgcgctgacg ttgcgtcgtg atatcatcag
    27121 ggcagaccgg ttacatcccc ctaacaagct gtttaaagag aaatactatc atgacggaca
    27181 aattgacctc ccttcgtcag tacaccaccg tagtggccga cactggggac atcgcggcaa
    27241 tgaagctgta tcaaccgcag gatgccacaa ccaacccttc tctcattctt aacgcagcgc
    27301 agattccgga ataccgtaag ttgattgatg atgctgtcgc ctgggcgaaa cagcagagca
    27361 acgatcgcgc gcagcagatc gtggacgcga ccgacaaact ggcagtaaat attggtctgg
    27421 aaatcctgaa actggttccg ggccgtatct caactgaagt tgatgcgcgt ctttcctatg
    27481 acaccgaagc gtcaattgcg aaagcaaaac gcctgatcaa actctacaac gatgctggta
    27541 ttagcaacga tcgtattctg atcaaactgg cttctacctg gcagggtatc cgtgctgcag
    27601 aacagctgga aaaagaaggc atcaactgta acctgaccct gctgttctcc ttcgctcagg
    27661 ctcgtgcttg tgcggaagca ggcgtgttcc tgatctcgcc atttgttggc cgtattcttg
    27721 actggtacaa agcgaatact gataagaaag agtacgctcc ggcagaagat ccgggcgtgg
    27781 tttctgtatc tgaaatctac cagtactaca aagagcacgg ttatgaaacc gtggttatgg
    27841 gcgcaagctt ccgtaacatc ggcgaaattc tggaactggc aggctgcgac cgtcttacca
    27901 tcgcacctac actgctgaaa gagctggctg agagcgaagg ggctatcgaa cgtaaactgt
    27961 cttacaccgg cgaagtgaaa gcgcgtccgg cgcgtatcac tgagtccgag ttcctgtggc
    28021 agcacaacca ggatccaatg gcagtagata aactggcgga aggtatccgt aagtttgcta
    28081 ttgaccagga aaaactggaa aaaatgatcg gcgatctgct gtaatcattc ttagcgtgac
    28141 cgggaagtcg gtcacgctac ctcttctgaa gcctgtctgt cactcccttc gcagtgtatc
    28201 attctgttta acgagactgt ttaaacggaa aaatcttgat gaatacttta cgtattggct
    28261 tagtttccat ctctgatcgc gcatccagcg gcgtttatca ggataaaggc atccctgcgc
    28321 tggaagaatg gctgacatcg gcgctaacca cgccgtttga actggaaacc cgcttaatcc
    28381 ccgatgagca ggcgatcatc gagcaaacgt tgtgtgagct agtggatgaa atgagttgcc
    28441 atctggtgct caccacgggc ggaactggcc cggcgcgtcg tgacgtaacg cccgatgcga
    28501 cgctggcagt agcggaccgc gagatgcctg gctttggtga acagatgcgc cagataagcc
    28561 tgcattttgt accaactgcg atcctttcgc gtcaggttgg tgtgattcgc aaacaggcgc
    28621 tgatccttaa cttacccggt cagccgaagt ctattaaaga gacgctggaa ggtgtgaagg
    28681 acgctgcggg taacgttgtg gtacacggta tttttgccag cgtaccgtac tgcattcagt
    28741 tgctggaagg gccatacgtt gaaacggcac cagaagtggt tgctgcattc agaccgaaga
    28801 gtgcaagacg cgacgttagc gaataaaaaa atccccccga gcggggggat ctcaaaacaa
    28861 ttagtgggat tcaccaatcg gcagaacggt gcgaccaaac tgctcgttca gtacttcacc
    28921 catcgccaga tagattgcac tggcaccaca gatcagacca atccagccgg caaagtggat
    28981 gattgcggcg ttaccggcaa tgttaccgat cgccagcagg gcaaacagca cagtcaggct
    29041 aaagaaaacg aattgcagaa cgcgtgcgcc tttcagcgtg ccgaagaaca taaacagcgt
    29101 aaatacgccc cacagaccca ggtagacacc aaggaactct gcatttggcg catcggtcag
    29161 acccagtttc ggcatcagca gaatcgcaac cagcgtcaac cagaaagaac cgtaagaggt
    29221 gaatgcggtt aaaccgaaag tgttgccttt tttgtactcc agcagaccgg caaaaatttg
    29281 cgcgatgccg ccgtagaaaa tgcccatggc aagaataata ccgtccagag cgaaataacc
    29341 cacgttgtgc aggttaagca gaatggtggt catgccgaag cccatcaggc ccagcggtgc
    29401 cggattagcc aacttagtgt tgcccataat tcctcaaaaa tcatcatcga atgaatggtg
    29461 aaataatttc cctgaataac agtagtgttt tcagggcgcg gcataataat cagccagtgg
    29521 ggcagtgtct acgatctttt gaggggaaaa tgaaaatttt ccccggtttc cggtatcaga
    29581 cctgagtggc gctaaccatc cggcgcaggc aggcgatttg cagtacggct ggaatcgtca
    29641 cgcgataggc gctgccgctg accgctttaa ccccatttaa tgctgcgccc acagggcctc
    29701 ccagacccgc gccgcgcagc aaaccatgcc caagcacgct cattgctgcg tgggtgcgta
    29761 aaatgcgggt cagttggctg gaaagcaaat gcgacacgcc ttttgccaat aatttgtctt
    29821 tcatcagcag cggcagcagc tcttccagct cattcaccct cgcatcgacc gcgtgcagaa
    29881 actcctgttt atgttcctcg tccatattct tacaggtgtt acgcagaaat tgttccagta
    29941 actgttgctc aatctcaaac gtagacatct ctttgtcggc tttcagcttc aatcgttttg
    30001 aaacatcgag caaaatggcc cgatacaatt taccgtgtcc gcgcagtttg ttggcgatac
    30061 tatcgccacc aaaatgctgt aattctccag caatcagctg ccagttgcgg cgatgttgct
    30121 cgggatgccc ttccatcgat ttaaacagtt cgttgcgcat cagtacgctg gagaggcgag
    30181 ttttgccttt ttcattatgg gtgagcaatc gggcgaaatt tgccaactgt tcctcactgc
    30241 aatgctggag aaaatccaga tctgaatcat tcaggtaatt aacattcatt ttttgtggct
    30301 tctatattct ggcgttagtc gtcgccgata attttcagcg tggccatatc cgatgagttc
    30361 accgtatgac cggaaaaggt gatttttgag acgcagcgtt tattgtcgtt atcgctgtta
    30421 atgttgatcc agtcagtggt ttgcccttct tttatttctg aaggaatatt caggctctga
    30481 ctggcgctac gggcggcttt gaaataaacc gatgcaccgc ttaactgtaa atcgccatgg
    30541 tcggcagaga gttgtatgcg tttaacaatt cggcaaacag gaagtttcag cgccagatcg
    30601 ttggtttcgt tacgtggcat cgcaatgacg ccgaggattt tatggtcgtt tgcctgcgcc
    30661 gtgcagcaca gcatcagact aatcgccagg ctggcggaaa tcgtaaaaac ggatttcata
    30721 aggattctct tagtggggag aggtaggggg atggataccc actagtttac tgctgataaa
    30781 gagaagattc aggtgcgtaa tgttttcttt ttattacaat tttttgatga atgtcttggc
    30841 tgcgattcat tctttatatg aataaaattg ctgtcagttt cacgtcttgt cctgctatat
    30901 cgcgaaattt ctgcgcaaaa gcacaaaaaa tttttgcatc tcccccttga tgacgtgggt
    30961 tacgacccca tttagtagtc aaccgcagtg agtgagtctg caaaaaaatg aaattgggca
    31021 gttgaaacca gacgtttcgc ccctattaca gactcacaac cacatgatga ccgaatatat
    31081 agtggagacg tttagatggg taaaataatt ggtatcgacc tgggtactac caactcttgt
    31141 gtagcgatta tggatggcac cactcctcgt gtgctggaga acgccgaagg cgatcgcacc
    31201 acgccttcta tcattgccta tacccaggat ggtgaaactc tggttggtca gccggctaaa
    31261 cgtcaggcag tgacgaaccc gcaaaacacc ctgtttgcga ttaaacgcct gattggtcgc
    31321 cgcttccagg acgaagaagt acagcgtgat gtttccatca tgccgttcaa aattattgct
    31381 gctgataacg gcgacgcatg ggtcgaagtt aaaggccaga aaatggcacc gccgcagatc
    31441 tctgctgaag tgctgaaaaa aatgaagaaa accgctgaag attacttggg tgaaccggta
    31501 actgaagctg ttatcaccgt accggcatac tttaacgatg ctcagcgtca ggcaaccaaa
    31561 gacgcaggcc gtatcgctgg tctggaagta aaacgtatca tcaacgaacc gaccgcagct
    31621 gcgctggctt acggtctgga caaaggtact ggcaaccgta ctatcgcggt ttatgacctg
    31681 ggtggtggta ctttcgatat ttctattatc gaaatcgacg aagttgacgg cgaaaaaacc
    31741 ttcgaagttc tggctaccaa cggtgatacc cacctgggtg gtgaagactt cgacagccgt
    31801 ctgatcaact acctggttga agaattcaag aaagatcagg gcattgacct gcgcaacgat
    31861 ccgctggcaa tgcagcgcct gaaagaagcg gcagaaaaag cgaaaatcga actgtcttcc
    31921 gctcagcaga ccgacgttaa cctgccgtac atcactgcag atgcgaccgg tccgaaacac
    31981 atgaacatca aagtgactcg tgcgaaactg gaaagcctgg ttgaagatct ggtaaaccgt
    32041 tccattgagc cgctgaaagt tgcactgcag gacgctggcc tgtccgtatc tgatatcgac
    32101 gacgttatcc tcgttggtgg tcagactcgt atgccaatgg ttcagaagaa agttgctgag
    32161 ttctttggta aagagccgcg taaagacgtt aacccggacg aagctgtagc aatcggtgct
    32221 gctgttcagg gtggtgttct gactggtgac gtgaaagacg tactgctgct ggacgttacc
    32281 ccgctgtctc tgggtatcga aaccatgggc ggtgtgatga cgacgctgat cgcgaaaaac
    32341 accactatcc cgaccaagca cagccaggtg ttctctaccg ctgaagacaa ccagtctgcg
    32401 gtaaccatcc atgtgctgca gggtgaacgt aaacgtgcgg ctgataacaa atctctgggt
    32461 cagttcaacc tggatggtat caacccggca ccgcgcggca tgccgcagat cgaagttacc
    32521 ttcgatatcg atgctgacgg tatcctgcac gtttccgcga aagataaaaa cagcggtaaa
    32581 gagcagaaga tcaccatcaa ggcttcttct ggtctgaacg aagatgaaat ccagaaaatg
    32641 gtacgcgacg cagaagctaa cgccgaagct gaccgtaagt ttgaagagct ggtacagact
    32701 cgcaaccagg gcgaccatct gctgcacagc acccgtaagc aggttgaaga agcaggcgac
    32761 aaactgccgg ctgacgacaa aactgctatc gagtctgcac tgactgcact ggaaactgct
    32821 ctgaaaggtg aagacaaagc cgctatcgaa gcgaaaatgc aggaactggc acaggtttcc
    32881 cagaaactga tggaaatcgc ccagcagcaa catgcccagc agcaacatgc ccagcagcag
    32941 actgccggtg ctgatgcttc tgcaaacaac gcgaaagatg acgatgttgt cgacgctgaa
    33001 tttgaagaag tcaaagacaa aaaataatcg ccctataaac gggtaattat actgacacgg
    33061 gcgaagggga atttcctctc cgcccgtgca ttcatctagg ggcaatttaa aaaagatggc
    33121 taagcaagat tattacgaga ttttaggcgt ttccaaaaca gcggaagagc gtgaaatcaa
    33181 aaaggcctac aaacgcctgg ccatgaaata ccacccggac cgtaaccagg gtgacaaaga
    33241 ggccgaggcg aaatttaaag agatcaagga agcttatgaa gttctgaccg actcgcaaaa
    33301 acgtgcggca tatgatcaat atggtcatgc tgcgtttgag caaggcggta tgggcggcgg
    33361 cggttttggc ggcggcgcag acttcagcga tatttttggt gacgttttcg gcgatatttt
    33421 tggcggcgga cgtggtcgtc aacgtgcggc gcgcggtgct gatttacgct ataacatgga
    33481 gctcaccctc gaagaagctg tacgtggcgt gaccaaagag atccgcattc cgactctgga
    33541 agagtgtgac gtttgccacg gtagcggtgc aaaaccaggt acacagccgc agacctgtcc
    33601 gacctgtcat ggttctggtc aggtgcagat gcgtcagggt ttctttgccg tgcagcagac
    33661 ctgtccacac tgtcagggcc gcggtacgct gatcaaagat ccgtgcaaca aatgtcatgg
    33721 tcatggtcgt gttgagcgca gcaaaacgct gtccgttaaa atcccggcag gggtggacac
    33781 tggagaccgc atccgtcttg cgggcgaagg tgaagcgggc gagcatggcg caccggcagg
    33841 cgatctgtac gttcaggttc aggttaaaca gcacccgatt ttcgagcgtg aaggcaacaa
    33901 cctgtattgc gaagtcccga tcaacttcgc tatggcggcg ctgggtggcg aaatcgaagt
    33961 accgaccctt gatggtcgcg tcaaactgaa agtgcctggc gaaacccaga ctggtaagct
    34021 gttccgtatg cgcggtaaag gcgtcaagtc tgtccgcggt ggcgcacagg gtgatttact
    34081 gtgccgcgtt gtcgtcgaaa caccggtagg cctgaacgag aagcagaaac agctgctgca
    34141 agagctgcaa gaaagcttcg gtggcccaac cggtgagcac aacagcccgc gctcaaagag
    34201 cttctttgat ggtgtgaaga agttttttga cgacctgacc cgctaacctc cccaaaagcc
    34261 tgcccgtggg caggcttggg taaaattagg gtgcgttgaa gatatgcgag cacctgaaaa
    34321 gtggcgggga tca
//
LOCUS       DAJOSY010000039        34247 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJOSY010000039 DAJOSY010000000
VERSION     DAJOSY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.039"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(47..988)
                     /gene="fabY"
                     /locus_tag="OHN27_001992"
                     /gene_synonym="yiiD"
     CDS             complement(47..988)
                     /gene="fabY"
                     /locus_tag="OHN27_001992"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCQ2286434.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1033..1470)
                     /gene="dtd"
                     /locus_tag="OHN27_001993"
     CDS             complement(1033..1470)
                     /gene="dtd"
                     /locus_tag="OHN27_001993"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCQ2286435.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1467..2339)
                     /locus_tag="OHN27_001994"
     CDS             complement(1467..2339)
                     /locus_tag="OHN27_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCQ2286436.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2333..2932)
                     /gene="yihX"
                     /locus_tag="OHN27_001995"
     CDS             complement(2333..2932)
                     /gene="yihX"
                     /locus_tag="OHN27_001995"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCQ2286437.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3031..3816)
                     /locus_tag="OHN27_001996"
     CDS             complement(3031..3816)
                     /locus_tag="OHN27_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2286438.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3850..4746)
                     /gene="yihV"
                     /locus_tag="OHN27_001997"
     CDS             complement(3850..4746)
                     /gene="yihV"
                     /locus_tag="OHN27_001997"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCQ2286439.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            4914..5810
                     /gene="yihU"
                     /locus_tag="OHN27_001998"
     CDS             4914..5810
                     /gene="yihU"
                     /locus_tag="OHN27_001998"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCQ2286440.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5834..6712
                     /gene="yihT"
                     /locus_tag="OHN27_001999"
     CDS             5834..6712
                     /gene="yihT"
                     /locus_tag="OHN27_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCQ2286441.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6729..7970
                     /gene="yihS"
                     /locus_tag="OHN27_002000"
     CDS             6729..7970
                     /gene="yihS"
                     /locus_tag="OHN27_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCQ2286442.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMDTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8108..9010
                     /locus_tag="OHN27_002001"
     CDS             8108..9010
                     /locus_tag="OHN27_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCQ2286443.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQLWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLESGKPHRLFFNIYGQRK"
     gene            9209..11245
                     /gene="yihQ"
                     /locus_tag="OHN27_002002"
     CDS             9209..11245
                     /gene="yihQ"
                     /locus_tag="OHN27_002002"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCQ2286444.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSCIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDAAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            11291..12676
                     /locus_tag="OHN27_002003"
     CDS             11291..12676
                     /locus_tag="OHN27_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2286445.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            12719..14122
                     /locus_tag="OHN27_002004"
     CDS             12719..14122
                     /locus_tag="OHN27_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2286446.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILIGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            14190..14882
                     /gene="ompL"
                     /locus_tag="OHN27_002005"
     CDS             14190..14882
                     /gene="ompL"
                     /locus_tag="OHN27_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCQ2286447.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYNFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(14973..16238)
                     /locus_tag="OHN27_002006"
     CDS             complement(14973..16238)
                     /locus_tag="OHN27_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2286448.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFSGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(16340..17320)
                     /locus_tag="OHN27_002007"
     CDS             complement(16340..17320)
                     /locus_tag="OHN27_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCQ2286449.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASYKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(17328..18038)
                     /locus_tag="OHN27_002008"
     CDS             complement(17328..18038)
                     /locus_tag="OHN27_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCQ2286450.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(18255..20078)
                     /gene="typA"
                     /locus_tag="OHN27_002009"
     CDS             complement(18255..20078)
                     /gene="typA"
                     /locus_tag="OHN27_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCQ2286451.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            20451..21860
                     /gene="glnA"
                     /locus_tag="OHN27_002010"
     CDS             20451..21860
                     /gene="glnA"
                     /locus_tag="OHN27_002010"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCQ2286452.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22034..23083
                     /gene="glnL"
                     /locus_tag="OHN27_002011"
     CDS             22034..23083
                     /gene="glnL"
                     /locus_tag="OHN27_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCQ2286453.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23095..24504
                     /gene="glnG"
                     /locus_tag="OHN27_002012"
     CDS             23095..24504
                     /gene="glnG"
                     /locus_tag="OHN27_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCQ2286454.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            24616..24726
                     /locus_tag="OHN27_002013"
     CDS             24616..24726
                     /locus_tag="OHN27_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCQ2286455.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(24955..26328)
                     /gene="hemN"
                     /locus_tag="OHN27_002014"
     CDS             complement(24955..26328)
                     /gene="hemN"
                     /locus_tag="OHN27_002014"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCQ2286456.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(26517..27026)
                     /gene="yihI"
                     /locus_tag="OHN27_002015"
     CDS             complement(26517..27026)
                     /gene="yihI"
                     /locus_tag="OHN27_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCQ2286457.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            27608..28240
                     /locus_tag="OHN27_002016"
     CDS             27608..28240
                     /locus_tag="OHN27_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCQ2286458.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(28621..31407)
                     /gene="polA"
                     /locus_tag="OHN27_002017"
     CDS             complement(28621..31407)
                     /gene="polA"
                     /locus_tag="OHN27_002017"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCQ2286459.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            31771..32703
                     /locus_tag="OHN27_002018"
     CDS             31771..32703
                     /locus_tag="OHN27_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCQ2286460.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(32744..34174)
                     /locus_tag="OHN27_002019"
     CDS             complement(32744..34174)
                     /locus_tag="OHN27_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCQ2286461.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8341 a         8765 c         8386 g         8755 t
ORIGIN      
        1 tataaatgac ctgaatgaga gaggcagcac cgcccctctc catcagctac tcttcttcgt
       61 tcccgccctc ttcatacggg ccaaatggct tcgcgggcag aacgatatac gtgccttcaa
      121 acactgcacc cggcgtctcg tcgccaaaga tttcgacctg catctgcacc cgtgcttttc
      181 gtccgcgcgc cagacggtcg agatcgccgc ttaaggcacc gaggtcggct accgcatgag
      241 gtttaccgct aatcggcttg ctgtagcgga tatgcgcatc cgcaagaata atcgttccgc
      301 cgaggtggcg ttcacgcagc atcagccaga taagtcccca accggtgagc gtcgccagtg
      361 agaataaact cccggcaaac agcgtatggt gcggattctg attgccggtt tctggcatgg
      421 tagtgataaa tttttgcccg gtatattgct gaatgcgcac gcccattttt tcactaagcg
      481 ggatatgttc gtaccacgcc tgttgcagct gcgcgcacca gtcgccgcga tgcagaatgt
      541 catccagagt ggcgacgggc ttaatcatca aaaaatggcg aatcggcgtg gtggttggcg
      601 tggtgatttc tccctgatta acaaacccca gcttggcgaa aaactccacc gcgtcttcac
      661 gggcgctaca ggtcacgcgc ttaacgcctt cctgacgcgc caccgactcc agggtcatcg
      721 ccatcagcgt gcctaaccct ttgtcctgca cgtcgggatg aacggccata aagcgaatgg
      781 acgcttcatt gtcggcatta atatacagtc ggcctaccgc caccagatta ccctgctcgt
      841 cgacgaccat ctggtgatgc gccatcgcat cccacgcgtc gcgttccgaa ccttttggtt
      901 gatgcagggg cttacgcaac atttcccagc gaaactgata gtaacgctct aattcttctt
      961 ctgtttgtgg aacccgaagg tgatacatag cgatactctc tcttgtttcc cgtgacaacc
     1021 ctggaagctg gctcatacct gcaaccagaa tgtcacgggg ccatcattga ccagcgatac
     1081 ctgcatatcc gcagcgaagc gtcctgtttg cgtgttcatc tcttgctgac ggcagcgttc
     1141 gacgaaatag tcatataacg cctctgcgcg atccggtgat gcacctttgg agaaacttgg
     1201 gcgcatcccc cgttcggtat ctgcggcgag ggtaaactgg gaaaccacca gcacactgcc
     1261 gcccgcctgt tgcacgttga gattcatctt gccttcggca tcgctaaaga tgcggtagcc
     1321 gagcacacgc tcgcacagac ggtttgcttt ctgttcgtcg tcatcctttt cgacacccaa
     1381 taacaccaaa agtcccgcgc caatttcgcc cgtcacttct ccctccacgg tgacgctggc
     1441 acgggttacg cgttgaatta atgcaatcat ggttcgtcgt cttcttcttg ttcagctgct
     1501 tgttttagtt tgcggtattc cccgagagtg acagtaattt ccgcgccaag caagacgata
     1561 caccatgtcc agtagaccca aacaaagaga atggggatca ccgccagcac accgtaaatg
     1621 agctgatatg acgggaacat ggtgatataa agcgcgaaac ctttctttcc tgcttcgaac
     1681 aggagtgcgg cgacaaacgc gccgacaatc gcgtcgcggt taggtacgcg gatggtagga
     1741 acaatgctgt acagcaacca gaaggagatc cacgacaaca gcaacggaaa aatacgcagc
     1801 acgttgtcga tgacagtatt gagatcgctc gcccagcgca gggagagcaa ataggaactg
     1861 atcgccagac tggcccctgc cagcagcggc cccagcgtta aaatcatcca gtacacggcg
     1921 aacgagtaaa tttttggtcg cgctcgttta ctgcgccaga tggtattcaa cgcgctatcg
     1981 atggagtaca tcaacaataa cgcggtgacg atcagcccgc acgccccaac ggcggtcatc
     2041 ttgttggaat tggcaacaaa ttgttcgata taccgctgaa taacatcgcc agtagcaggc
     2101 agaaagttgg caaaaataaa gtgacgtaac tgaatgctga cgtcggaaaa catgggaaaa
     2161 gcggcgaaaa gcgcaaaaac aacggcaacc agcggcacta atgagagcaa cgacacatag
     2221 gcaaggttac ctgccagggt tgtcatgttg tcctcatcaa tgcgttgcca gagtagtttt
     2281 agccaggccc atagtggacg ggtacggtgc ctggctttgt cctgaatggt ttttagcata
     2341 acaccttcgc gaaatagtcc gggatggtgg ttttatcttt caccagaata ctggtaatgc
     2401 ccagctgatt ggctccttct atattatcgg cgttatcgtc gaaaaagacc gtatcgctgg
     2461 gtgaaaaacc ttccgcctgc aaaacatgct ggtaaattcg tgcttcaggt ttgcgcatcc
     2521 ccagatcttg cgacagatag atatggtcag cagcatcacg aatttccggg tattcttccg
     2581 gccagaaggt ggtatgcagg cggttggtat tggaaagcac caccacgcga tgcccctgct
     2641 cacgcagttt atgcatgatg gcgatcactt ccgggcgcag cgcaacaaac accgcctgcc
     2701 agccgtgaga gaactgctcg tagcttagcg gtagagccat ctcatgacac agcgcctctg
     2761 cgaacgcttc gtcgctaatt tccccacgct catgctgatg aaacgcctcc cccatatgaa
     2821 aactcttctt aagcgatgcc agcggaatac gcgttaaatc gctccaggct cccagcacac
     2881 ggttaaagtc gatatcgaca atcacattac ctaaatcaaa gatatagagc atttttgcct
     2941 cctttcgcgc cacaagaaat taactgtagc gggaaaggat gggtttggct atgtggcaag
     3001 gtatgaagcc aggtcaacag tcacaaaaat ctacgcgtct tcctggggaa ggatgatttc
     3061 aatttggctg tcttgtaagc gtgattttag cgcagcgggc ggcagctcgt cggtaaagag
     3121 cgcagtgacc tgtgccacgt taccaatttc aaccgccgcc gaagcatgat acttagtgtg
     3181 atcggcgacc agcagaatat ttctcgcgtg cgccatcatc gttttcacca cgttagcttc
     3241 gtttacatca aactccatca acgcgccatc gctctcaatc gccccaacgc ttgttaccag
     3301 ataatcagca cgaaaatcag ccacaaagga cgccgctgaa gggccaatga tcccgctatt
     3361 atgagagcgc aacgtaccgc cgggcaccat cacttcaaag cgcgggttgt ggtaaagaat
     3421 atgcgccaca cgcaggctgt tggtgattat ccgcaaatga ttatggttaa gtaacgcccg
     3481 ggcaacatgc tcaacagtcg taccaatggt gataaatatt gttgaaccat caggaatata
     3541 gtctgccacc gcttcggcaa tcgctttttt ttcctcggtt tgcgaaacct cacgctgctc
     3601 gaacgccgta ttaacgacgc tggaagcccg acccgcgcca ccgtgatggc gcgtaatcag
     3661 gccttgctcg cttaatttac gaatatcccg gcggaccgtc tgcgtggaga catccagcag
     3721 atttgccagc tcatcaatat tcatataccc acgctcggcg atcagcatga ggagttggtc
     3781 gtgccgcggg ttaccggtta gttcggtaag gctcatgaaa attcctcgaa aaaccatcac
     3841 cctggcattt tatacaaaaa gtgacaaaaa agatcgggtt tgatcacagt cagggatccc
     3901 ggctcgtcca ccgggacgtg tgcattttaa cgccgctaca ccgctggcga agcggactga
     3961 ctccgctaaa tccccacttg ttgccagcgc caccgccaaa gcgccgtgaa aaacatcacc
     4021 cgcacctgtg gtatctacca catcaacttt gaaggccggt tgatgctgac gcccaccatt
     4081 ttccagccag tcgcagcctg cgctaccctg ggtcacatag acatgaccat ttgtgagcgt
     4141 ttgtgcctgt tttagcgcac tggccatctc tttcacgccc gttaagcgcg ccagacccgg
     4201 ttctgaaaag gccgcgtgat cgcttaatgc caccagctca ctgatatcct gcggcgtaat
     4261 gtccccgtcc agaacggtca tcacacccgc ctgacgggcc agggtgaagg cttttttagc
     4321 gccgtcgtgc cagcgtacat ctgccagcac aacatcccac tgagagaaat caatttcctc
     4381 caaccactct gcgtcaggca gcagatccgg gctggggtag ttaatgatta tccgctcgcc
     4441 tttggtatcc accatgatgg cggattgcga agatttcgcc tggttatacc gtttggtgta
     4501 acgggtgtta acgccccagg attccagctc tgccagcagg ctgttgcccg tgtcgtcatc
     4561 acctacgcga ccaataaaat cgacctgcgc ccccagcctt gccgccgcaa ccgctgccgt
     4621 cgccgctggc ccgccaccaa cttccgtata atttctcgcc acgtatttac cgctctccgt
     4681 cggtaaccct tccacgtaat agatgcgatc catcacggtt atacctacac aagcaacacg
     4741 aatcatggtt attccttaaa cattttggga tgcgttcatt ttaatttcac caattgttta
     4801 aaaagtgacg gctgtcaatt ttttgacgat aaattacaaa tacgatcaaa aacagacaag
     4861 caaagcatgt cataaatgac aaaaagtgac atcactgtat tcaggagagg gttatggcag
     4921 caatcgcgtt tatcggttta ggacaaatgg gttcgccaat ggcgagcaat ttattgcagc
     4981 aagggcacca acttcgcgtc tttgatgtga atgccgaggc tgtgcggcat ctggtagaca
     5041 aaggcgcgac tcccgccgcc aacccggcgc aggccgctaa agatgccgaa tttatcatta
     5101 ccatgttgcc gaatggcgat ctggtgcgca gcgtgttgtt cggtgagaac ggcgtttgcg
     5161 aaagcttatc taccgatgcg ctggtcattg atatgtcgac catccatccg ctgcaaaccg
     5221 ataaattgat tgccgatatg caagccaaag gcttcagcat gatggatgtt ccggtaggcc
     5281 gtacttctgc aaatgccatt accggtactc tgttactgct ggctggaggc accgctgaac
     5341 aagttgaacg tgccacgccg atcctgatgg cgatgggcag tgagttgatc aacgctggcg
     5401 gtccgggcat ggggatccgc gttaagctca tcaacaacta tatgagcatc gcgctcaatg
     5461 cgctttcggc agaagctgcc gttttgtgcg aagccctgaa tcttcccttc gatgttgccg
     5521 tcaaagtgat gagcggtacc gccgccggta aaggccactt caccacttcc tggccaaaca
     5581 aagtcctcag cggagatctt tctcccgcct tcatgatcga tcttgcccat aaggatcttg
     5641 gcatcgccct tgatgtcgcc aaccagctgc atgtgccaat gccgctgggg gccgcctcac
     5701 gggaggttta tagccaggcg cgcgcagcgg gtcgcggtcg ccaggactgg tccgccattc
     5761 tggaacaggt ccgtgtcagt gccgggatga ctgccaaagt aaaaatgtaa cgactggata
     5821 aaggaataaa tgaatgaata agtacaccat caacgacatt acgcgcgcat cgggcggttt
     5881 tgccatgctg gcggtcgatc agcgcgaagc catgcgcatg atgtttgccg cggctggggc
     5941 acccgctccg gtagccgata gcgttttaac tgatttcaaa gttaacgctg caaaggccct
     6001 ctcgccttat gcctcggcga ttctggtaga tcaacaattc tgctaccgcc aggtggttga
     6061 gcaaaacgcg attgccaaaa gttgcgccat gattgtcgcc gccgatgaat tcattcctgg
     6121 caacggtatt ccggtcgata gcgtggttat tgaccgcaaa atcaacccgc tacaaatcaa
     6181 acaagacggt ggcaaagcct taaaactgct ggtgctgtgg cgtagtgatg aagatgcgca
     6241 gcaacgtctg gatatggtga aagagttcaa cgaactgtgc cactcacacg gtctggtaag
     6301 cattattgag ccagtcgtcc gcccaccgcg tcgtggcgat aaattcgatc gcgaacaagc
     6361 gatcatcgat gccgccaaag agctgggcga cagtggcgct gacctctaca aagttgaaat
     6421 gccgctttat ggcaaaggtc cgcaacaaga gcttctctgt gcttcacaac gtctgaatga
     6481 ccatatcaat atgccatggg tgatcctctc ttccggtgtc gacgaaaaac tgttcccgcg
     6541 tgccgtacgc gtggcaatga cggcgggcgc atcgggattc ctggcaggcc gtgcagtctg
     6601 ggcatcggtc gtcggtttac cagacaacga gctgatgctg cgtgacgttt gcgcaccgaa
     6661 attacaacaa cttggcgata tcgtcgacga aatgatggct aaacgccgct aagatgagga
     6721 taaaaggaat gaaatggttt aacaccctaa gccacaaccg ttggctggaa caggaaaccg
     6781 accgcatctt tgattttggt aaaaattccg tagtgccgac tggttttggc tggttaggca
     6841 ataaagggca aatcaaagaa gagatgggca cccatctgtg gatcaccgct cgtatgttgc
     6901 acgtttattc cgttgctgcg gcgatgggtc gacctggcgc ttactcgttg gttgatcacg
     6961 gtatcaaagc catgaacggc gcactgcgtg ataaaaaata tggcggctgg tatgcctgcg
     7021 tgaatgacga gggcgtggtg gatgcctcca aacagggcta tcaacacttc tttgctctgc
     7081 tgggtgccgc cagcgccgtc acaacgggtc acccggaagc gcgcaagctg ctcgattaca
     7141 ccattgaaat tatcgagaaa tatttctgga gcgaagaaga gcagatgtgc ctggaatcct
     7201 gggacgaagc cttcagcaaa accgaagagt accgcggcgg caatgccaat atgcacgcgg
     7261 tggaagcttt cttgattgtt tatgacgtca ctcacgacaa aaaatggctg gatcgcgcga
     7321 ttcgcgtggc ttccgtgatt atccacgacg tcgccagaaa taatcattat cgcgttaacg
     7381 aacacttcga tacccagtgg aatccgctgc cggattacaa caaagataac ccggcgcatc
     7441 gcttccgcgc gttcggaggt acaccaggcc actggatcga atggggccgt ttaatgctgc
     7501 acatccatgc tgccctggaa gcccgttgcg aacaaccacc agcatggctg ctagaagatg
     7561 ccaaaggtct gtttaacgcc accgtgcgcg atgcctgggc acccgatggc gcggacggga
     7621 ttgtttatac cgttgactgg gaaggaaaac cggtggtccg cgaacgtgta cgttggccta
     7681 tcgtcgaagc aatggatacg gcctacgcgc tctacaccgt cactggcgat cgccagtatg
     7741 aaacctggta tcaaacatgg tgggagtact gcattaagta cctgatggac tatgaaaatg
     7801 gttcctggtg gcaggagctg gatgcggaca ataaggtcac caccaaagtc tgggacggca
     7861 aacaggatat ttatcacctg ctgcattgcc tggtgatccc gcgtatcccg ttagcgcccg
     7921 gcatggctcc agcggttgca gcgggtctgc tggatattaa tgcgaaataa ctgatctgaa
     7981 aatagggtgc gggttttccc ctctccctaa ccctctcccc aaaggggcga ggggaccgtc
     8041 cggtgcagtt tttgggtttg acatcagcct cacgggaggc tgtatgtcgt taattaaggt
     8101 gccttgtatg caaatcacca atatgcattg cagtggacag accgtttcac ttgccgcggg
     8161 ggattatcac gcgaccattg tgaccgtcgg tgctggtctg gctgaattaa cctttcaggg
     8221 ttgtcattta gtcatcccgc acaaacccga agagatgccg ctggcgcatc tgggtaaagt
     8281 gcttattccg tggccgaacc ggatcgccaa cggctgttac cgctatcagg ggcaggaata
     8341 tcagttaccc attaatgaac atagttcaaa agccgctatt cacgggctgc ttgcctggcg
     8401 agactggcaa attagtgaat taaccgccac cagcgttacc cttacagctt ttctgccgcc
     8461 cagttacggc tatccgttta tgctggcttc acaagttgtt tattcgctca acgcacacac
     8521 gggtctttcc gttgagatcg caagccagaa tatcggcact gtcgcagcgc cttatggcgt
     8581 gggtattcat ccttatttaa cctgcaactt aacatctgtt gatgaatatc tgttccagct
     8641 tccggcaaac caggtttatg ccgtcgatga gcacgcgaat ccgacaacgc ttcaccacgt
     8701 tgatgagctg gatttaaatt tcacgcaggc gaaaaaaata gccgcgacaa aaatcgacca
     8761 cacctttaaa accgctaatg atttgtggga aatgaccatt acccatccac agcaggcgct
     8821 ttcggtaagc ctttgttccg atcaactctg ggtgcaggtt tatagcggtg aaaaattaca
     8881 gcgtcaggga ctggcagttg aacctatgag ttgcccgcct aacgccttta attcaggtat
     8941 cgacttatta ttactggaat caggaaaacc gcaccgcctg ttttttaata tttacggaca
     9001 gagaaaataa cttgtattga ttagcagtca aaatcattga agaacatttc actcgctaaa
     9061 ggcaacatta tgtatcttca atttattaag ttaaaaacag ctcagcttct gtaaagtttt
     9121 tatatatcag ttttatgaga atcattttat ataagcctgt aataacagga aggcaacaat
     9181 ctcgttctca actctatgga gattagttat ggatacgcca cgtccacagt tattagattt
     9241 tcaatttcat cagaataacg acagttttac cctacatttt caacaacgtc ttattttaac
     9301 ccatagcaaa gataatcctt gtttatggat tggctcaggt atagcggata tcgatatgtt
     9361 ccgcggtaat ttcagcatta aagataaact acaggagaaa attgcgctta ccgacgccat
     9421 cgtcagccag tcaccggatg gttggttaat tcatttcagc cgtggttctg acattagcgc
     9481 cacgctgaat atctctgccg acgatcaggg ccgtttattg ctggaactac aaaacgacaa
     9541 ccttaaccac aaccgcatct ggctgcgcct tgccgcacag ccggaagatc atatctacgg
     9601 ctgcggtgaa cagttttcct actttgatct gcgcggcaaa ccgttcccac tgtggaccag
     9661 tgaacaaggc gttggtcgca acaaacaaac ctatgtcacc tggcaggccg actgcaaaga
     9721 aaatgcgggc ggcgactatt actggacttt cttcccgcag cctacgtttg tcagcacgca
     9781 gaagtattac tgccatgttg ataacagttg ctacatgaac ttcgacttta gtgccccgga
     9841 ataccatgaa ctggcgctgt gggaagacaa agcaacgctg cgttttgaat gtgctgacac
     9901 atacatctcc ctgttagaaa aattaaccgc cctgctggga cgccagccag aactgcccga
     9961 ctggatttat gacggggtga cgctcggtat tcagggcggc actgaggtgt gccagaagaa
    10021 actggacacc atgcgtaacg cgggcgtgaa ggtcaacggc atctgggcgc aggactggtc
    10081 cggtattcgt atgacctctt ttggcaaacg cgtaatgtgg aactggaagt ggaacagcga
    10141 aaactacccg caactggact catgcattaa gcagtggaac caggagggcg tgcagttcct
    10201 ggcctatatc aacccgtatg ttgccagcga taaagacctc tgcgaagaag cggcaaagcg
    10261 cggctatctg gcaaaagatg ccgccggtgg cgattatctg gtggaatttg gcgagttcta
    10321 cggcggcgtt gtcgatctca ctaatcctga agcctacgct tggttcaagg aagtgatcaa
    10381 aaagaacatg attgaactcg gctgcggcgg ctggatggct gacttcggcg agtatctgcc
    10441 caccgacact tacctgcata acggcgtcag cgccgaaatt atgcataacg cctggcccgc
    10501 gctgtgggcg aagtgtaact acgaagccct tgaagaaacg ggcaagctcg gcgagatcct
    10561 tttctttatg cgcgccggtt ctaccggtag ccagaaatac tccaccatga tgtgggcggg
    10621 cgaccagaac gtcgactgga gtctcgacga tggcctggcg tcggttgtcc cggcggcgct
    10681 gtcgctggca atgaccggac atggcctgca tcacagcgac attggcggtt acaccaccct
    10741 gtttgagatg aagcgcagca aagagctgtt gctgcgctgg tgcgatttca gcgccttcac
    10801 gccgatgatg cgcacccacg aaggtaaccg tcctggcgac aactggcagt ttgacggcga
    10861 cgcagaaact atcgcccatt ttgcccgtat gacctccgtc ttcaccaccc tgaaacctta
    10921 cctgaaagag gctgtcgcgc tgaatgcgaa gtccggcctg ccggttatgc gcccgctgtt
    10981 cctgcattac gaagacgatg cgcacactta caccctgaaa tatcagtacc tgttaggtcg
    11041 cgacattctg gtcgctccgg tgcatgaaga aggccgtagc gactggacgc tctatctgcc
    11101 ggaggataac tgggtccacg cctggacggg tgaagcgttc cggggcgggg aagttaccgt
    11161 taatgcgccc atcggcaagc cgccggtctt ttatcgcgcc gatagcgaat gggcggcact
    11221 gttcgcgtcg ttaaaaagca tctaatcaca tccgcccgtg gggaaactca tgggcaaagg
    11281 gagaacatcg atgagtcaca tcacaacgga agatccagca actttacgcc tgccctttaa
    11341 agagaaactc tcttacggta ttggcgacct ggcctctaac atcctgctag atatcggtac
    11401 gctttatctt ttgaagtttt ataccgacgt tctggggctg ccaggcacct atggcggcat
    11461 tatctttttg atttcaaaat tctttactgc gtttaccgat atgggtaccg gcattatgtt
    11521 ggattcccga cgcaagatcg gtccaaaagg taagttccgt ccttttattc tgtatgcgtc
    11581 attcccggtc accttactgg cgatcgccaa ctttgtcggc acaccgtttg atgtcaccgg
    11641 taaaacggtg atggccacta ttctgtttat gctttacgga ctgtttttca gcatgatgaa
    11701 ctgctcctac ggcgcaatgg tcccggcaat caccaaaaac cccaacgaac gcgcctcact
    11761 ggcggcatgg cgtcagggcg gcgctacgct gggcctgctg ctgtgcacgg tgggattcgt
    11821 gccagttatg aatcttatcg aaggtaatca gaaacttggc tatatcttcg ccgccacgct
    11881 gttttcactg ttcggcctgc tgtttatgtg gatctgctac tcaggcgtga aagagcgtta
    11941 tgtcgaaacc caaccaacca atccggcgca aaagcctggc ctgttgcaat ctttccgcgc
    12001 aattgccggt aaccgcccac tgttcattct gtgtattgcc aacctctgca ccttaggggc
    12061 gtttaacgtc aagctcgcca ttcaggtcta ttacacccag tacgtactta acgatcccat
    12121 cctgttgtcg tatatgggat ttttcagcat gggctgtatt ttcatcggcg tgttcctgat
    12181 gcctggcgca gtcagacgtt ttggtaagaa gaaggtctat atcggcggcc tgctgatttg
    12241 ggtgctgggc gatctgctca actatttctt cggcggcggc tcggtcagct ttgtggcgtt
    12301 ctcctgcctg gcgttcttcg gctcagcgtt tgttaacagc ctgaactggg cgctggtttc
    12361 cgacaccgtc gagtacggcg agtggcgcac cggcgtgcgt tcggaaggca cggtctacac
    12421 cggcttcacc ttctttcgca aagtgtctca ggcgctggct ggtttcttcc ccggctggat
    12481 gctgacgcaa attggctatg tgccgaacgt cgcacaggct gaccacacca ttgaagggtt
    12541 acgccagttg atcttcatct acccaagcgc actggcggta gtcaccattg tggcgatggg
    12601 ttgcttctac agcctgaacg agaagatgta tgtccgcatt gtggaagaga tagaagcccg
    12661 taaacgcacg gcgtaataat aataaacgac gccctgcggg gcgttataag gagtgattat
    12721 gtctgaccat aatccactga cattaaaact gaatctgcgg gaaaaaatcg cctatggtat
    12781 gggcgacgtc ggttcgaatt taatgctctg catcggtact ctgtatctcc tcaaatttta
    12841 taccgatgaa ttagggatgc ctgcttacta tggcgggatt atctttctgg tggcgaagtt
    12901 ttttaccgcg tttaccgata tgctcaccgg atttttactc gactcgcgga aaaatattgg
    12961 gcctaaaggc aaatttcgtc cctttatttt atatgcagcg gttccggcgg cgttaattgc
    13021 aacgctccag tttatcgcca ccaccttttg tttgccggtt aaaacgacga ttgccaccgc
    13081 gctgtttatg atgtttggac tttcatacag tctgatgaac tgctcgtatg gtgcgatgat
    13141 cccggcaatt accaaaaacc cgaatgagcg cgcgcagctc gcggcttacc gccagggtgg
    13201 tgcaaccata gggctattga tttgtaccgt ggcgtttatt ccgttgcagt cgcttttttc
    13261 tgactcaacc gtcggttatg cctgtgcggc acttatgttc tccattggcg gctttatttt
    13321 tatgatgctg tgctacagag gcgtcaaaga gcattatgtg gacacaacgc caaccggaca
    13381 taaagccagt attctcaaat cattttgcgc gatatttcgg aatccgccat tgctggtttt
    13441 atgcattgct aacttgtgta ccctggcggc atttaatatc aaactggcga ttcaggtcta
    13501 ttacacccag tatgtgctga atgatattaa tttattgtcg tggatgggat tcttcagtat
    13561 gggatgcatc ctcatcggcg tattactggt gccattaact gtaaaatgtt ttggtaaaaa
    13621 acaggtttat ctggctggca tggtgctgtg ggcggtgggt gatatactga attatttctg
    13681 gggaagtaac tctttcactt tcgtcatgtt ctcttgtgtc gccttttttg gcacggcctt
    13741 tgtaaacagc ctgaactggg cactggtacc agataccgta gattacggtg aatggaaaac
    13801 cggtattcgt gccgaaggtt ctgtttatac cggttatacc ttctttcgta aaatttctgc
    13861 cgcacttgct ggcttcttgc caggcattat gctgacgcaa attggttatg ttcccaacat
    13921 cgcgcaaagc gatgcgacat tacagggttt gcgtcagctc atttttatct ggccttgtgc
    13981 actggcaatt attgctgcat tgacgatggg attcttttac acactcaatg aaaaacggtt
    14041 tgcattgatt attgaggaaa tcaaccaacg caaaaataaa gagatggcga cagaagaaaa
    14101 aacggcttcc gtaacgttat aaatgtcaat agccgctatt tccatcctgg tgggtggcgg
    14161 cctccctacg tttaaaaaat ggacttatta tgaaaaagat taatgcaata attttattat
    14221 cttctctgac ctctgcctcg gtatttgctg gcgcatacgt cgaaaacagg gaggcatata
    14281 atcttgcctc cgatcagggc gaggttatgc tacgtgtggg ttataacttc gatatgggtg
    14341 cgggtattat gttaaccaat acctacaact ttcagcgaga agatgaacta aaacatggat
    14401 ataacgaaat tgaaggctgg tatccgttat ttaaaccaac cgataaatta accatccagc
    14461 ccggtggctt aattaatgat aagagtatcg gttcgggtgg tgcagtgtat ctggacgtca
    14521 actataaatt tgtaccatgg tttaatctga cagtacgaaa tcgctataac cataataact
    14581 atagttcaac agatttgagc ggggaactgg ataataatga cacctatgaa attggcacct
    14641 actggaattt taaaatcacc gataaatttt cctatacatt tgagccacat tacttcatgc
    14701 gagtgaatga ctttaatagt agcaacggga aagatcatca ttgggaaatc actaacacct
    14761 tccgttaccg tattaatgaa cactggctcc cttatttcga attgcgctgg ttagaccgca
    14821 acgtcgaacc gtaccaccgc gagcaaaacc agatccgtat cgggacgaag tatttcttct
    14881 gatgtgaaac tgccggatgc ggcgtaaacg ccttatccgg cctacggttg atacggtttt
    14941 cattggccgg attaagcaca tccggccatt aattaagctt cagccgccag catctctttc
    15001 ttacgttggc taatacgctt aaccagcatt acggaaacca ccgcaccact gaatgcgaag
    15061 caggccataa tgccaaacac gattttgtag ccaataatcc ccggatttaa atccagaatg
    15121 tagccataca gactgaagca gaacatcgcc ggagcgtaac caataaagct acccaacgcc
    15181 atcgccgcac ctgttttatt ttcagcaatt tttgcttcgc cgataggtgc aaaaaatacg
    15241 gctcgctgtg taaagactat cgcgccaaag cccagcgtac atgccatccc taaatagacc
    15301 ggcatacttt cgtgcggcag cataatcaac agtacgagcg cagcggtact aatgataaag
    15361 gtgtagcata gatatttact tggcgatttc aggatcttat ctgaaatcat gccaccaatc
    15421 ggtccaccaa tcattttcag acagtattgg ttaatgatgc cgtaagcccc caccagcgca
    15481 acgggcaata gatagatgtt tttcaggaat ggaatgaaga atgtcaggcc acagtaaacc
    15541 gcgtaaacga agaagacgtt aaaagcgata agccagatag ttttgtcttt cagcaccgag
    15601 gtcatactgg tgtttttcga tgctccgtct tcttttttca cctcaacgga cggtgcctct
    15661 tctttgtcat tcagaacaaa gaaaatgata atgcctacgg caatcacaat aagggaatag
    15721 aaccagatgc ctgctttaaa acccaataag ccactgccaa accaggtaaa tactgccaac
    15781 gcagaaaatg ccaccacggt atcgacaatg ccacgccctg tttcgaagaa gccaaacaac
    15841 cgaccttgtt gttcgctatt gcccaatcga cttaccgact tgagcaagac cggccagttc
    15901 atcatgtcgc aagtaacgcc aaatagcgcc cagacaaaga ggatgcccca ataccccggc
    15961 attgtcgtca aataaacacc cagtaatcct gtcgcaatga gtgagaatga catggtgtat
    16021 ctgcgcggta gtttatcggc aaaataaata gacagaaaaa aacccactgt cgtgacaaat
    16081 gagtttaccg acatagcatt accaatttga ccattggtca aatggaaata ttcctgcatc
    16141 gggatataaa acgcatcttt cagcgacggt aatttataaa ttgtaccgcc acacaatgtt
    16201 aatagactaa ataacgccca tttctttttc gtgagcataa catcccctaa gcgattttta
    16261 aaatcactgt tttataggca ataagatacc gaccattcat atggaatggt tttattaact
    16321 gcgtatgagt taaaacttat tagtggttca gaagatggtt tgcctcttgt ttgatttgtt
    16381 gtagtacgtt acgcacatga tttatctgat taattgcatc ttctttttct ttacgtaacc
    16441 gcgcatccag taaatgattt tctggcaatg gtgtttcact catctggcga taagggatcc
    16501 acggattatc atcttcgtct tcaaagcgga acgccggcgc atagtaatca acctcttctt
    16561 ccaggccata tgccgtcacc tgcggctcat catcacccag gcagataagg tgcgttaata
    16621 acgctttgaa cggcatatca ccctgacctg aaatacaggc tttatgaccc aggccaccct
    16681 gttctttaac gatcaaggca tctttgatat ggacctgggt aatatgcggt gccatcgttt
    16741 ttaaggcgtc gatgggatgc tcatttgcat tgatcatgtt cgcaaaatca aacagtaagg
    16801 aaagagactc catctcactt tctttgacca gcgacaccag ctcatgactt tttaaatctt
    16861 catgctgctc gatcgtaaaa gtcaggccgc tgtcctgata cgtttcccgt acataggcaa
    16921 tgtcgttagc gataatcgat aatacgtcgc gcaaattacc ttcataacgt ggataaaaac
    16981 gtacggacga tgccccagtt ttcaacgcga tggcgacggc ttcgtcaata gatgccttat
    17041 atgaggcgct ggtttcaata tgaatatcaa ggttcagacg gcgggcttta tctccaaagg
    17101 cagagagttc tttatcgtcc atattaccaa gagaaaaacg ctcgccatcc agaacatgga
    17161 ttttgacgcc acgtagatta ttttcacttg cgatatcaag taaatcagca ggcaacactc
    17221 tttccagccg catatttaaa tgaaaggcat aagcatgtaa ataaagagga agagtgtcga
    17281 cacgttgtag aatctttctt gcgttattta ttgtcaccat aaaacaccta cagattaatg
    17341 atgaggtcta cggcctcaaa tcgccaatat tcaatatcta tctggacaat attgccttgc
    17401 tggtcggcgc gatgcttttc aattaagatc gctggcatac ccgcagaacc gccaagatac
    17461 ttgctgatat cgcccggcat tctgacgggt ttgaaaatca atttttttac taccgtctct
    17521 ttttggtagc acttttccca gactgcagaa aatgagtggt tttcaagttg ttcaataaaa
    17581 cccggagcaa cttcaggatt aatatatgtt tcgtgataga aaactttatg tccttccagc
    17641 gccccccagc cggtgatgcg atagagttca gttgatggtg tcactgcaat cagtggcgcg
    17701 agcgtctcgg gaatatcgct ggtacggttt ttctcggtaa acccccagga aggctctcgt
    17761 ccttgctcaa ttgcggcccg ctgaaagctc gctgacagct ccggactgta attaaatcgc
    17821 ggctgggtca cgaaccaccc cttacgatcc ttacggaaga ttttggattc tgcctcgagg
    17881 ttcaacaacg cctggcgcag cgtcatacgt ttaatgccca gcagttctcc cagctctctt
    17941 tctgaaggga gttttccacc cggcgtggtg atgccatctt tcaaccaccg atccagtttc
    18001 tcttttgcat tttctacggt ggattgattc tccgccattg tttaatcctt ttggtttaaa
    18061 ccagatgaag catcttacgc ttacgccaaa aaagaaagca attaacggcg tatgactttg
    18121 cagataattg tgacaaagct ctctattgac gtaatcccag acttataagg gagatactcg
    18181 cattggtgtg cgctcacgac aggtaaaaaa aaacctgcca gcgatggcag gttttttata
    18241 actaaaaaac gaaattaatc gtctttcggt gcgcggttgg cgcgacgacg atcgttttca
    18301 gtcaggtgac gtttacgaat acggatagag gtcggagtca cttctaccag ttcgtcatca
    18361 tcgatgaact ccagagcttg ttccagagtc atgcggatag gcggaaccag aacaacggct
    18421 tcgtcagtac cggaagcacg catgttggtc agtttcttac cagtcaggca gtttaccgtc
    18481 aggtcgttag agcggctgtg aataccgata atctgacctt cgtaaacttc tgcaccgtga
    18541 ccgaggaaca gcttaccgcg atcctgcaga ccgaacagcg cgaacgcgac cgctttaccc
    18601 tgaccgttag agatcagtac gccgttctga cgctgaccca cttcacccgg acgtacgtcg
    18661 tcgtagtggc tgaaggtgga gtacagcaga ccagtaccgg aagtcatggt catgaactca
    18721 gaacggaagc caatcagacc acggcttggg atcacgtagt cgagacgtac gcggccttta
    18781 ccgtctggat tcatgttttt caggtcgcct ttacgttcgc ccagcgcctg cattacagaa
    18841 ccctgatgct gttcttcaac gtccagagtc acgttttcat acggctcttg tttacgaccg
    18901 tcgatttcac ggaagataac tttcggacgg gataccgcca gttcgaaacc ttcacgacgc
    18961 atgttttcga tcagaacaga caggtgcagt tcgccacgac cagaaacgcg gaacgcatcg
    19021 gcgtcttcgg tttcttctac gcgcagcgca acgttgtgta ccagttcttt gttcagacga
    19081 tccaggatct gacgagacgt tacgaactta ccttctttac cgcagaacgg cgaggtgtta
    19141 acgcagaaga acatagaaac ggtcggctca tcaacggaga gtgccggcag cgcttcaacg
    19201 ttttgcgtgt cgcaaacggt gtcagaaatg ttcagttcgc caaggcccgt gatcgcaacg
    19261 atatcgccag cttccgccag atcggtttcg atacgttcca gaccgaggtg gcccagcact
    19321 ttaccgactt tcgcgttgcg ggttttgcct tcgctatcga tgatagtgac ctgctggttc
    19381 ggcttcactt taccgcgctt gatgcggcca atgccgataa cgccaacata gctgttgtaa
    19441 tcgagctgag aaatctgcat ctggaacgga ccgtcaaggt caacgtccgg cgcaggaacg
    19501 tgatcaacaa tcgcctgata cagcggggtc atgtcttccg ccatatcttc gtggtccaga
    19561 cccgcgatac cgttcagcgc agaagcgtaa acgatcggga agtccagctg ctcgtcggtc
    19621 gcgtcgaggt taacgaacag atcgaatacc tgatccacaa cccaatcagg acgcgcgcca
    19681 gggcggtcaa ctttgttgat aacaacaatc ggcttcaggc cgtaagcaaa cgcttttttg
    19741 gttacgaagc gcgtttgcgg catcgggccg tcaaatgcgt caaccaccag cagcactgag
    19801 tctaccatgg acattacacg ttcaacttca ccaccgaagt cggcgtgccc cggggtatca
    19861 acgatgttga tacggtaatc attccatttg atagcggtgt ttttcgcgag gatggtaatc
    19921 ccacgctctt tctccaaatc gttggagtcc atcacgcgct cttgggtttc ggcacgagag
    19981 tcgaacgtac cggattgttg gagcagcttg tctaccaggg tggttttacc atggtctacg
    20041 tgcgcgatga tggcgatatt acgcaatttt tcgatcacaa ctttgcctca ggcattagaa
    20101 atagcgcgtt attgtacacg gattaatcgc actacaaaac aggatcacaa acatcctccg
    20161 caaacaagta ttgcagagtc cctttgtgat cgctttcacg gagcataaaa agggttatcc
    20221 aaaggtcatt gcaccaacat ggtgcttaat gtttccattg aagcactata ttggtgcaac
    20281 attcacatcg tggtgcagcc cttttgcacg atggtgcgca tgataacgcc ttttaggggc
    20341 aatttaaaag ttggcacaga tttcgcttta tcttttttac ggcgacacgg ccaaaataat
    20401 tgcagatttc gttaccacga cgaccatgac caatccagga gagttaaagt atgtccgctg
    20461 aacacgtact gacgatgctg aacgagcacg aagtgaagtt tgttgatttg cgcttcaccg
    20521 atactaaagg taaagaacag cacgtcacta tccctgctca tcaggtgaat gctgaattct
    20581 tcgaagaagg caaaatgttt gacggctcct cgattggcgg ctggaaaggc attaacgagt
    20641 ccgacatggt gctgatgcca gacgcatcca ccgcagtgat tgacccgttc ttcgccgact
    20701 ccaccctgat tatccgttgc gacatccttg aacctggcac cctgcaaggc tatgaccgtg
    20761 acccgcgctc cattgcgaag cgcgccgaag attacctgcg ttccactggc attgccgaca
    20821 ccgtactgtt cgggccagaa cctgaattct tcctgttcga tgacatccgt ttcggatcat
    20881 ctatctccgg ttctcacgtt gctatcgacg atatcgaagg cgcatggaac tcctccaccc
    20941 aatatgaagg tggtaacaaa ggtcaccgtc cggcagtgaa aggcggttac ttcccggttc
    21001 cgccggtaga ctctgctcag gatattcgtt ctgaaatgtg tctggtgatg gaacagatgg
    21061 gcctggtggt tgaagcccat caccacgaag tagcgactgc tggtcagaac gaagtggcta
    21121 cccgcttcaa taccatgacc aaaaaagctg acgaaattca gatctacaaa tatgttgtgc
    21181 acaacgtagc gcaccgcttc ggtaaaaccg cgacctttat gccaaaaccg atgttcggtg
    21241 ataacggctc cggtatgcac tgccacatgt ctctgtctaa aaacggcgtt aacctgttcg
    21301 caggcgacaa atacgcaggt ctgtctgagc aggcgctgta ctacattggc ggcgtaatca
    21361 aacacgctaa agcgattaac gccctggcaa acccgaccac caactcttat aagcgtctgg
    21421 tcccgggcta tgaagcaccg gtaatgctgg cttactctgc gcgtaaccgt tctgcgtcta
    21481 tccgtattcc ggtggtttct tctccgaaag cacgtcgtat cgaagtacgt ttcccggatc
    21541 cggcagctaa cccgtacctg tgctttgctg ccctgctgat ggccggtctt gatggtatca
    21601 agaacaagat ccatccgggc gaagccatgg acaaaaacct gtatgacctg ccgccagaag
    21661 aagcgaaaga gatcccacag gttgcaggct ctctggaaga agcactgaac gaactggatc
    21721 tggaccgcga gttcctgaaa gccggtggcg tgttcactga cgaagcaatt gatgcgtaca
    21781 tcgctctgcg tcgcgaagaa gatgaccgcg tgcgtatgac tccgcatccg gtagagtttg
    21841 agctgtacta cagcgtctaa tagttgaagt tgtactaccc ggcgcaacaa cgccgggatt
    21901 tagttgccgt ggaaactttc agcccatctc tgcatgggct tttttctccg tcaattctct
    21961 gatgcttcgc gctttttatc cgtaaaaagc tataatgcac taaaatggtg caacctgttc
    22021 aggagactgc tttatggcaa caggcacgca gcccgatgct gggcagatcc tcaactcgct
    22081 gattaacagt attttgttaa tcgatgacaa cctggcgatc cattacgcca accccgccgc
    22141 gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt acaccgttac cggaactgtt
    22201 gagctacttc tcattaaata tcgagctgat gcaagaaagt ctggaggcgg ggcaaggttt
    22261 taccgataac gaagtgacgc tggtcatcga cgggcgctcg catatccttt ctgtgacggc
    22321 ccagcgtatg ccggacggca tgatcctgct ggagatggct ccgatggata accagcgccg
    22381 cttaagtcag gaacagctac agcacgccca gcaggttgct gcccgtgatt tagtgcgcgg
    22441 cctggcacat gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc agctgctcag
    22501 caaagcgtta cctgacccat cactactcga atataccaaa gtgattatcg aacaggcgga
    22561 ccggctgcga aatctggtcg accgtctgtt ggggccgcag ctgcccggta cgcgcgttac
    22621 cgaaagtatt cacaaagtgg ctgaacgcgt ggtaacgctg gtgtcgatgg aactgccgga
    22681 caacgtgcgg ttgattcgtg attacgatcc cagcctgccg gaactggcgc acgacccgga
    22741 tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc tggggccgga
    22801 aggcggtgaa atcattctgc gtacccgcac cgcgtttcaa ctgaccttac acggcgagcg
    22861 ctaccggctg gcggcgcgga ttgatgtgga agataacggg ccgggcattc cgcctcattt
    22921 gcaggatacg ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg ggcttggctt
    22981 atccatcgct cgtaatttga ttgatcagca ttcaggcaaa attgaattta ccagttggcc
    23041 agggcatacc gagttctcgg tttacctgcc tatcaggaaa taaaggtgac gtttatgcaa
    23101 cgagggatag tctgggtagt cgatgacgat agttccatcc gttgggtgct tgaacgtgcg
    23161 ctcgctggag cgggtttaac ctgtacgaca tttgagaacg gcgcggaagt actggaggcg
    23221 ctggcgagca aaacgccgga tgtgctgctt tcagatattc gtatgccggg aatggacggg
    23281 ctggcgctgc tcaagcagat taaacagcgc catccaatgc ttccggtcat cattatgacc
    23341 gcacattccg atctggatgc tgccgtcagc gcctatcaac aaggggcgtt tgattatctg
    23401 cccaaaccgt ttgatatcga cgaagcagtg gcgctggttg agcgcgctat cagtcattac
    23461 caggaacagc agcagccgcg taatattcag cttaacggcc caacgaccga tatcatcggc
    23521 gaagcgccag ccatgcagga cgtgttccgt attatcggtc ggctttcgcg ttcttctatt
    23581 agcgtgctga ttaacggcga atccggcacc ggtaaagaac tggtcgctca tgccctgcat
    23641 cgccacagtc cgcgcgccaa agcgccgttt atcgcgctga atatggcagc tatcccaaaa
    23701 gatttgatcg aatcagaact gtttggccac gagaaaggcg cgtttactgg cgcgaatacc
    23761 attcgtcagg ggcgttttga acaggccgat ggcggtacat tattcctcga cgaaattggt
    23821 gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc tggcagacgg tcagttttac
    23881 cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc cactcaccag
    23941 aatctcgaac agcgagtgca ggaaggtaag ttccgtgagg atctgttcca ccgcctgaac
    24001 gttatccgcg ttcatctgcc gccgctgcgc gaacgtcggg aagatattcc ccgtctggcg
    24061 cgccattttt tacaggttgc cgcgcgcgaa ctgggcgtag aagcgaagtt gctgcatccg
    24121 gaaaccgaag ctgctctgac gcgtctggcg tggccaggca acgtacgcca gctggaaaac
    24181 acctgccgct ggctaacggt gatggccgcc gggcaggaag tgttgattca ggatttgccc
    24241 ggcgaactgt ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc ggacagttgg
    24301 gcgacacttt tagcgcagtg ggcagacaga gcgctgcgtt ccggtcatca aaatctgctt
    24361 tccgaagcgc agccagagct ggagcggacg ttactgacga ccgcgttgcg acatacgcag
    24421 gggcataaac aggaagcggc gcggctactc ggctggggcc gcaacaccct gacgcgtaag
    24481 ttaaaagagc tggggatgga gtgattcaca gcttgtgtgt aaagattgat tattgagcgc
    24541 aaattgctgg tattttgcgc tttactgttc cgataagttc agtatgatct tgcccggaaa
    24601 acggggagag tcattatgct ggaatcaata attaatctgg tatcgagtgg cgcagttgac
    24661 agccacacac cgcaaactgc cgttgctgcc gtgctgtgtg ccgcgatgat tgggctgttt
    24721 agctgaagct cgttgtttat gccggatgcg gagaaagtcg taggcctgat aagacgcgac
    24781 cagcgccgca tcaggcatca tgctcagatg cctgatgcgg cgtaaacgcc ttatccggcc
    24841 tacaaaagca ataatttcaa caggttttgt tgcccagcgc atcaggcaat cttgttctca
    24901 gcacattgat ggcgctttcg ttttctactt tgtaaacgaa gcgccattca ctatttaaat
    24961 cacccgagag aactgctgca tccgcgcttt ctggcgcaga taggtatcaa agcacatgca
    25021 aatgttgcgg atcagcaagc gacctttcgc cgtcacctgt attcccttct catccacatc
    25081 caccagccca tcttttgcta acggggcgag cagcttgaga tcttccgcaa agtaatcagc
    25141 gaagtgcaaa tcccactgtt tctcaatagg ggcgtaatcc agacggaagt tgcagatgag
    25201 cgacttaatc acatcgcggc gaatacagtc atcacgcgtt agcgcaatac cacgccacag
    25261 cgcattgcct tgttcatcca cttgctgata gtactgcttc aactctttct ggttctgcgc
    25321 gtagcagtcg ccaatcatgc tgatggcgga aacgcccatc cccagcagat cggtatcgcc
    25381 ctgagtggtg tagccctgga agttacgatg cagcacgcct tcacgctggg ccaccgccag
    25441 ctcgtcatcc ggacgggcaa agtgatccat accgataaac tgatagcccg attgcgtcag
    25501 gaaggcgatg gtttcctgca ggatatcgag tttttgctgc ggactcggca ggtcagcatc
    25561 tttgattttg cgctgagcag caaaaatggt cggcagatgc gcgtagttaa agacactcag
    25621 acgatcgggg ttcagctccg ccacacgttt cagggtaaag gcgaaactct ccggcgtctg
    25681 tttcggcagg ccgtaaatca ggtcgatgtt ggtggaggta aaaccaatct cacgcgcatg
    25741 gttaagcagt gcaaagatga actcttcatc ctgctcgcgg ttaaccagac gttgcacttc
    25801 tttgttgaag tcctgcacgc ccatgctcag gcgattaaag ccttcggcgc gtaaatgatc
    25861 gagtacatcc agttcgattt cccgcggatc gacttcgatc gaaatctccg catcggcatt
    25921 gaactggaag ttttcgcgca gcagcttcat caggcggctg atttgcgctt tattcagata
    25981 cgtcggcgtt ccgccgcccc agtgcaattg gctgacgtga cgcccggcga acagcggtgc
    26041 acgatggacg atttcttgct ccagcgcgtc cagatactga tcggccttgt gctgctggcg
    26101 agtaacaatc ttattgcaac cgcagaagta acaaagctta tggcagaacg ggatatgtac
    26161 gtagagagat aatggacgct caggatagcg cgccacggct tgtaaaaacg cctgttcgcc
    26221 gaagtcttct gaaaactcca gcgcggtcgg gtacgaggtg tatcgtggcc cggaatagtt
    26281 atatttctgg atcagggcca gatcccagtc gatttgctgt acagacatgc tcactccttc
    26341 cgatggcgtc tctggcggct acggcgtacc ggcggatgcc cgtttcgggt catcagccgg
    26401 ttgcgcagcc acttttgtcg ccgcgacaac cgctgtagtt taacgaataa ccacaccaga
    26461 taacatataa ccggaagggt tatcagcaga acgggaaggc ccacggcggc aatccgttag
    26521 ttgccccgca gcagacgcat catgtcttct tgcttctcgt cttcttcctc ttcttcgtca
    26581 tcgtcataag agaggccgag tttctgcatc aactcatcaa tacgatccag tttagcatcc
    26641 acccaggatt gctcttcggc actcagggtt tcgcctgctt ccagacgttc cagcagcgca
    26701 tccagacgct catccgtttc cagtaattcc aactccgcct gcggtgaaag cataggtttc
    26761 tcactcttcg gtttgtgctg tttggtgact ttttcagtca cgcccaatgg aataggggtt
    26821 ttactgccaa tacgtggatc ttttggtgcg ttctggcctt tgctgcctga cgtggtgttt
    26881 ccgcccgctg cgcggctgcc cggcgcatga ccacgacgtt ttttctgacg cttgcgatca
    26941 cgagcttcct gatccagctc ctcgcgtgtt tttcgacgcg cttttgcatg acctttgctg
    27001 cgtgagtttg aagatgatgg tttcataatg gttcgtcttt tagccgtttt attcagtata
    27061 gatttgcggc ggaatctaac agaaagcaag caaagaaaaa aggcgacaga ttactctgtc
    27121 gccttttttc ctgactcata acccttaacg ggtcttacaa tccttgcagg ccgccaacac
    27181 accctgtttt tcctttagcc ggaacaatcc gtgttgattc catttccgtt taattacgtc
    27241 tccggacgtt tgtcttcctg acaatcctct gtcttcgcct cctggcgctc ctgaccctca
    27301 tcctgagtca ttgttcctgt tagcgtcctc gctctatggg tgctacttta cgcctttgct
    27361 ttaaacaaac aatcaaccgc catcagtatt tgcacatttt cagagtttta tataaaagca
    27421 attcactgat ttatatagct tatttgacat gcatcgcaga attttctctg ctatttcgca
    27481 taaagactat cggcaatgtc tcacaaagcg tatggctttt acttacaacg ctcacgccga
    27541 aaaatccgcc ttttattatg gttcaggtat aatcgccaca aagctttgac tgacggagac
    27601 gaccgctttg actaatttga attatcaaca gacgcatttt gtgatgagtg cgcctgatat
    27661 tcgccaccta ccttccgata ccggaattga agtggctttt gcaggccgtt ccaacgcagg
    27721 taaatccagc gcgctgaaca cgctgactaa ccagaaaagc ctggctcgta cctcaaaaac
    27781 cccagggcgc acccagctta tcaacctgtt tgaagtggct gacggcaagc gtctggttga
    27841 cttgcctggg tacggttatg cggaagtccc ggaagagatg aagcgcaaat ggcagcgtgc
    27901 gctcggcgaa tacctcgaaa aacgtcagag cctgcaaggt ctggtggtgc taatggatat
    27961 tcgccatccg ctgaaagatt tggatcagca gatgattgag tgggcggtag acagcaatat
    28021 cgccgttctg gtgctgctga ccaaagcgga caaactggca agcggcgcac gtaaagcgca
    28081 attgaatatg gtgcgtgaag ctgtactggc gtttaacggt gatgtgcagg ttgaaacgtt
    28141 ttcttcgttg aagaaacaag gcgtggacaa gctgcggcag aaactggata cctggtttag
    28201 cgagatgcag cctgtagaag aaacgcagga cggcgaataa ttttcttgcc ttaatgcttg
    28261 tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt acaattctac cgttggcctg
    28321 ataaacatag cgcatcaggc attacggatc ttttctttcg cccaataaaa aacgccccag
    28381 tcattactga ctggggcggc taaaatattc agccaaatcc gattacgtga agtaaaaggt
    28441 ctgaaagata gaacatctta cctctgtacc ctacgcgact aactttactc ttttttgttc
    28501 agttcagaaa gcactttttg taattttttt tcagttgttg cataggaatt tctaatagcc
    28561 atcacaaaac gtgacagctt atgttgctta cttacgaaaa aaggcatgtt caggcgaatc
    28621 ttagtgcgcc tgatcccagt tttcgccact ccccacttcc accagcaacg gcacatccag
    28681 acgggtacag ttttccatca gttgatgaat ctgcttcgcg acggcatcaa catcatcttt
    28741 atgaacttca aataccagtt catcgtgtac ctgcatgatc atacgtacac gcggttgctc
    28801 agcctgtaac cacgcatcaa cggcaatcat cgcccgtttg ataatgtcgg cggcggttcc
    28861 ctgcattggc gcgttaatgg ctgcacgttc agccgctgca cgacgagcac cattgctgga
    28921 tttgatatcc ggcagataca gacggcgtcc gtccagcgtt tcaacgtagc cctgctcttt
    28981 cgcctgagca cgggtgcgtt ccatatactc cagcacgcca gggtagcgtt cgaagtaaag
    29041 gtccatgtac ttctgcgctt ctttacgtgg aatgttcaat tgccgcgcca gaccgaaagc
    29101 actcatgcca taaatcagac caaagttgat cgctttcgcg ctacggcgtt gctcgctggt
    29161 gacggtttcc agtggcaaac caaacacttc tgccgccgtt gcccggtgga tatcttttcc
    29221 ttccgcgaat gcggtcagca agcctttgtc acgcgaaaga tgcgccataa tgcgcagttc
    29281 aatctgcgag tagtccgctg agacaatcac ataatcctct ggcgcaataa acgcctggcg
    29341 gatacgacga ccttcttcgt tacgcaccgg aatgttttgc aggttaggat cggttgacga
    29401 taaacgtccc gttgcagtta ctgcctggtg ataagaggta tgcacacgcc cggttttcgg
    29461 gttgatcatc agcggcagct tgtcggtgta ggtcgatttc agcttcgcca gaccacgata
    29521 ctccagaatc acttttggca acggatagtc cagcgccagt tcttccagta cctcttccga
    29581 cgttgacggc gcgccacccg gcgttttctt cagcggttta atgccctgtt tttcaaagag
    29641 aatggtttgt aactgcttgg tggaagaaag gttaaattcc tcacctgcaa tttcatgcgc
    29701 tttcttttcc agctcagcca gacgaagggt gagctcttca gaatgattgt gcagcacttt
    29761 cggatcgatc ttcacaccgt tacgttcaat gcgtgaaagc accggcacca gcggcatttc
    29821 gatattctcg aagacgttca acggcccttt gtgtttttgc agatccggcc acattttcag
    29881 atgcaactgc aaggtgacat ctgcatcttc ggcggcgtaa cgtccggctt cttcgagggc
    29941 aatctggtta aaggtcagtt gatttttgcc tttaccagca atctcttcaa aagtgatggt
    30001 tttgtgcttc aaccaacgtt ccgcgaggct gtccatatcg tgacgcccgg caacgctatt
    30061 gagaatgtag gactccagca tggtatcaaa cgcaatccca cgcagttcaa tgccgtagtt
    30121 cgccagaata ccgcgatcgt atttcaggtt ttgcccgacc ttcagcgcct tttcatcttc
    30181 cagcagcggt tttagcaact cgagtgcacg ctcgcgagag atttgatcgg gcgcatcaag
    30241 ataatcatga gcaaccggaa tatatgccgc tacgcctggc tcgatagcaa aagaaagccc
    30301 gaccaggtta gcagagatgt tatcaaggct gtcggtttcg gtatcaaatg caaataccgg
    30361 cgctttttcc agcttcgcaa tccacgcttt cagtgtttct tcatcaagga tggtgacgta
    30421 gttgtcataa gaaatcaccg ttgccgtcac ttctggtgct tcgtctgcaa cactggtttc
    30481 ctgtggcttc gcggctggtt ttgccccttt ggcctgtaac catttgcccg cttcgacatc
    30541 agcagtccag cgtttgaact catacttttt gaacagcccc aacaactctt ccgctgccgg
    30601 ttgctgcact tccagttgtt cacaggtcag ctccagttca acgtcggttt taatcgtcgc
    30661 cagctggtat gagagataag caacttcttt gttttgctcg agcttcgctg ccattgtttt
    30721 cgcgccacgg aagctcaacc cagcaatttt ttctggctcg gcatacagcg tatccagtcc
    30781 gccaagacct tgcagcaatg cctgcgcggt tttttcaccg acgcccggta cgccaggaat
    30841 gttatcagag gagtcaccca tcagcgccag gaaatcgatg atcagttctg gcggcacgcc
    30901 gtacttattc accacctctt ccggtccgag gatggtattc gtcatggtat tgataagcgt
    30961 aatatttggc gtcaccagct gcgccatatc tttatcgcca gtgctgatca gcaccggacg
    31021 cccggctttt tcggcttcgc gcgccagagt accgataacg tcgtccgctt ctacgccaga
    31081 aaccgccagc agcggcagtc ccatcgcttt aaccatcgcg tgcaagggtt cgatttgtgc
    31141 acgcagatcg tccggcattg gcgggcgatg tgatttgtaa tgttcaaaca gttcatcacg
    31201 aaaggttttt cccttggcgt caaagaccac cgctgcatgc gtcggtttat attgcatgat
    31261 cagactgcgc agcatgttga ggacaccata catcgcaccg gtcggctcgc ctgcgctgtt
    31321 agtcagcggg ggaaacgcgt gatatgcgcg ataaagataa gatgaaccat ctacaaggat
    31381 aagtggattt tgggggatct gaaccataat gtccgtgcct gtttatcaga ttatgggtaa
    31441 aggatgccac agacaggatg aaaacatgag tttttcaccg cttttcgtga aaatcttttg
    31501 tagatcttct ggatcgctcg caaatcgtca tgtggataac tttgtgcata aattatttac
    31561 atcgctttta atttattatc atgcttttta cagcatgatg tttgtctttg tttttcatgc
    31621 agttaaaaat aaatccatcc ctaataattg agataatggg attttttcta atgtggatag
    31681 atatgaatta tttttctcct taaggatcat ccgttatttg ggtcgttttc tgctaaaatc
    31741 tgcgccttgc gcttctttag cgcactgtct atggctaacc ttttgaataa attcattatg
    31801 acgagaatac tcgctgcgat aacccttttg ctgagtatcg tattgaccat tttggtcact
    31861 attttttgtt ccgtcccgat catcattgcc gggattgtaa aactattgct acctgtgcca
    31921 gtcatttggc gaaaggtttc gcgtttttgt gattttatga tgtattgctg gtgtgaaggt
    31981 ctggcggtat tactgcatct gaacccacac ctgcaatggg aagttcacgg gctggaaggg
    32041 ttaagtaaga agaactggta tctgcttatt tgtaaccacc gtagttgggc agatattgtc
    32101 gtactgtgcg tgctgtttcg taagcacatt ccgatgaata aatatttcct caagcaacag
    32161 ctggcctggg tgccatttct tggcctggcg tgctggtcgc tggatatgcc gtttatgaag
    32221 cgttattccc gcgcttattt attacgtcat ccggagcgac gtggtaaaga tgttgaaacc
    32281 actcggcgtt cttgtgaaaa gtttcgcctg catcccacca ccattgtcaa tttcgttgaa
    32341 ggctcccgtt tcacgcaaga aaaacatcag caaacccact ccacttttca aaacttgttg
    32401 ccaccaaagg cggcaggcat tgcgatggcg ctaaatgtac tgggtaaaca attcgataaa
    32461 ctgcttaatg ttacgctgtg ttatccggac aataaccgtc agccgttctt cgatatgtta
    32521 agcggtaaac tgacgcggat tgtcgtacat gtggatttac agcctattgc cgatgagtta
    32581 catggcgact acatcaatga caaaagcttt aagcgccatt ttcagcaatg gctgaactca
    32641 ttgtggcagg aaaaagaccg gcttctgacg tctctcatgt catcacagcg tcagaataag
    32701 taagttttac tcgcctctga atgagcagag gcgagtgagt attttaatga acaaaacgtc
    32761 cggcacgaga cataaattct tcttcgctca tctcctgtcc gttaaaaaca actttacccg
    32821 gtgtataacg cagctgcagc gaggcggtat tgttgtcaat cgtgattaac tgaaacatct
    32881 gacctaatgt catcatcccg ctgatttgtt tatcagcctg cttttgcgct ttttccgcat
    32941 ccattccttc agataaatta agctgttttg cggtttctgt gacgacatta agcggtaact
    33001 tcacatcaaa attgagcgat ttgatatctt tgtttgtgga tgatgaagat ttggctgggt
    33061 cggcaatact gatatccaga ttggcattta gttcgccgag tgcgttcttc catcttaccg
    33121 gttgtttaat ggtcggctca cttttttgta ataacggtaa atactctttg aacaatgcag
    33181 cattcacttc ttgcagagca acttcatcgt ttgctaactc agggtgtgca gcaagctgct
    33241 tctgcatcgc aatgttatac tggatgataa attggcgcac tgctgaggga tcaatagatt
    33301 cagcaatcaa tgaccactca ccactaccga gttgctgatt cccttttgtt aacttatcaa
    33361 gttcgtaggt gagattgaca ttgatataat ctttgtcttt aacgcgatcc agcctggttt
    33421 ttgcaacgaa agcatcgatt tgggcaacgt cttccccgtg attgataatg ttaatctgat
    33481 taaatttcga ctcgctttca ccaaccggaa atttcttttc ttctaatcgc gccagagaat
    33541 caatggtaaa actttttgcc gttactttgt tcattggtga taattgcaga acaatatctt
    33601 cgactttgcc ttcaatatcc aggttagaca atgaactacc atcaccatta aaagtaaact
    33661 ggccctcacc aaaagaaaac tttccatact ctgcaggggc aacatctaat gtcgtagaga
    33721 atgagccacc aaacgcgaat cgcaatgtcg cttccacggg cgatttattt ttcgcggcga
    33781 taaatagcgg ttgtgtcagt tcattattca ctaagttcac ttttgccgct gccagtgctg
    33841 ggatgacatt accatgcatt aacattgtga tgggcaacgg accgtgctca acatccacat
    33901 caaatacaac tttctggcct ggcttgatat tgagatcggg tgcaccgtta tcgaatgtca
    33961 tttgcatttg aaaacgggaa ttaaaaaagc ctttgtcaaa attttgataa cttaacgtca
    34021 tatcgtaggc atgttcacct ttctttttag catcgttaaa atctttaata aatttttcga
    34081 caccaggctg aatttgcgta cctgtgtacc atgtgccacc accccagatt acggctaacg
    34141 caacaataac acctgtagct gacttacgta tcatataaat attccctgat aaaacagcaa
    34201 aactttgaat atccacttat gctgaaataa tgtccagcgg caggatg
//
LOCUS       DAJOSY010000040        34216 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJOSY010000040 DAJOSY010000000
VERSION     DAJOSY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.040"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(171..434)
                     /gene="cedA"
                     /locus_tag="OHN27_002020"
     CDS             complement(171..434)
                     /gene="cedA"
                     /locus_tag="OHN27_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCQ2286462.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            617..2878
                     /gene="katE"
                     /locus_tag="OHN27_002021"
     CDS             617..2878
                     /gene="katE"
                     /locus_tag="OHN27_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCQ2286463.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3136..3885)
                     /gene="chbG"
                     /locus_tag="OHN27_002022"
     CDS             complement(3136..3885)
                     /gene="chbG"
                     /locus_tag="OHN27_002022"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCQ2286464.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEQGIALRADRQMAFDLP
                     VNLRTTQGFSSAFYGEEISESLFLQVLDDAGHRGDRSLEVMCHPAFIDNTIRQSAYCF
                     PRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3898..5250)
                     /gene="celF"
                     /locus_tag="OHN27_002023"
                     /gene_synonym="chbF"
     CDS             complement(3898..5250)
                     /gene="celF"
                     /locus_tag="OHN27_002023"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCQ2286465.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5355..6197)
                     /gene="chbR"
                     /locus_tag="OHN27_002024"
     CDS             complement(5355..6197)
                     /gene="chbR"
                     /locus_tag="OHN27_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCQ2286466.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6205..6555)
                     /gene="chbA"
                     /locus_tag="OHN27_002025"
     CDS             complement(6205..6555)
                     /gene="chbA"
                     /locus_tag="OHN27_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCQ2286467.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6606..7964)
                     /gene="chbC"
                     /locus_tag="OHN27_002026"
     CDS             complement(6606..7964)
                     /gene="chbC"
                     /locus_tag="OHN27_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCQ2286468.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(8049..8369)
                     /gene="chbB"
                     /locus_tag="OHN27_002027"
     CDS             complement(8049..8369)
                     /gene="chbB"
                     /locus_tag="OHN27_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCQ2286469.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8668..9006)
                     /gene="osmE"
                     /locus_tag="OHN27_002028"
     CDS             complement(8668..9006)
                     /gene="osmE"
                     /locus_tag="OHN27_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCQ2286470.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9208..10035
                     /gene="nadE"
                     /locus_tag="OHN27_002029"
     CDS             9208..10035
                     /gene="nadE"
                     /locus_tag="OHN27_002029"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCQ2286471.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10265..11152
                     /gene="cho"
                     /locus_tag="OHN27_002030"
     CDS             10265..11152
                     /gene="cho"
                     /locus_tag="OHN27_002030"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCQ2286472.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11112..11687)
                     /gene="ves"
                     /locus_tag="OHN27_002031"
     CDS             complement(11112..11687)
                     /gene="ves"
                     /locus_tag="OHN27_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCQ2286473.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPFQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11890..12375)
                     /gene="spy"
                     /locus_tag="OHN27_002032"
     CDS             complement(11890..12375)
                     /gene="spy"
                     /locus_tag="OHN27_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCQ2286474.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKVKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12705..13673)
                     /gene="astE"
                     /locus_tag="OHN27_002033"
     CDS             complement(12705..13673)
                     /gene="astE"
                     /locus_tag="OHN27_002033"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCQ2286475.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13666..15009)
                     /gene="astB"
                     /locus_tag="OHN27_002034"
     CDS             complement(13666..15009)
                     /gene="astB"
                     /locus_tag="OHN27_002034"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCQ2286476.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDTVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(15006..16484)
                     /gene="astD"
                     /locus_tag="OHN27_002035"
     CDS             complement(15006..16484)
                     /gene="astD"
                     /locus_tag="OHN27_002035"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCQ2286477.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDAGAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16481..17515)
                     /gene="astA"
                     /locus_tag="OHN27_002036"
     CDS             complement(16481..17515)
                     /gene="astA"
                     /locus_tag="OHN27_002036"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCQ2286478.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17512..18732)
                     /gene="astC"
                     /locus_tag="OHN27_002037"
     CDS             complement(17512..18732)
                     /gene="astC"
                     /locus_tag="OHN27_002037"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCQ2286479.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTLNHRYGLFSEIRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19178..19984
                     /gene="xthA"
                     /locus_tag="OHN27_002038"
     CDS             19178..19984
                     /gene="xthA"
                     /locus_tag="OHN27_002038"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCQ2286480.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20151..20861
                     /locus_tag="OHN27_002039"
     CDS             20151..20861
                     /locus_tag="OHN27_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCQ2286481.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     FFGYSLYILLFIIATLLLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSNTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20866..21543
                     /gene="ydjY"
                     /locus_tag="OHN27_002040"
     CDS             20866..21543
                     /gene="ydjY"
                     /locus_tag="OHN27_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCQ2286482.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQQENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKIAE"
     gene            21558..22265
                     /locus_tag="OHN27_002041"
     CDS             21558..22265
                     /locus_tag="OHN27_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCQ2286483.1"
                     /translation="MMMMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22265..22813
                     /locus_tag="OHN27_002042"
     CDS             22265..22813
                     /locus_tag="OHN27_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCQ2286484.1"
                     /translation="MGLPPLSKIPLILRPQAWLHHRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22823..23989
                     /locus_tag="OHN27_002043"
     CDS             22823..23989
                     /locus_tag="OHN27_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2286485.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDVLHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23962..25497
                     /locus_tag="OHN27_002044"
     CDS             23962..25497
                     /locus_tag="OHN27_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2286486.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKWL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDIDQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGNTLAIFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFAVGIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25497..26150
                     /locus_tag="OHN27_002045"
     CDS             25497..26150
                     /locus_tag="OHN27_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCQ2286487.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAEQFSCTGELWLNEQRIDILPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLEGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            26217..27524
                     /gene="ynjE"
                     /locus_tag="OHN27_002046"
     CDS             26217..27524
                     /gene="ynjE"
                     /locus_tag="OHN27_002046"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCQ2286488.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGLTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNSVATGERGPDSSK"
     gene            complement(27533..28153)
                     /locus_tag="OHN27_002047"
     CDS             complement(27533..28153)
                     /locus_tag="OHN27_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCQ2286489.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPTCFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            28240..28647
                     /gene="nudG"
                     /locus_tag="OHN27_002048"
     CDS             28240..28647
                     /gene="nudG"
                     /locus_tag="OHN27_002048"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCQ2286490.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQRDQAGLWEFAGGKVGLDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28613..28885)
                     /locus_tag="OHN27_002049"
     CDS             complement(28613..28885)
                     /locus_tag="OHN27_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCQ2286491.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            29121..30464
                     /gene="gdhA"
                     /locus_tag="OHN27_002050"
     CDS             29121..30464
                     /gene="gdhA"
                     /locus_tag="OHN27_002050"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCQ2286492.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(30520..32463)
                     /locus_tag="OHN27_002051"
     CDS             complement(30520..32463)
                     /locus_tag="OHN27_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCQ2286493.1"
                     /translation="MNCWEILGLQPGVEERDIKRRYAKLVKNCRPEDSPEAWQQLHDA
                     YEQALRYDDEDQWQEEEEEQPINMSRGIVHPRPVMTFRAPNTFSGHPLPDVTIKSEGE
                     FTWQAVLDMLCEENETTPEHAPMQLAKALSFLEKKDLSSRMCFEEALLLHLHRVFRPL
                     LTLAAAQTFRWHLVAGSRQPAIQTEINESCLLYYRIEKQITPFFASNLSPLEEMENGK
                     EMATIYQSLKDNEESLKWFDIAVLNKVAEFELSDNYMALLIEYLSCSWRKEKMIRKWK
                     LTERFNNLAMQHHSGYKKLLNAIKLKGLFYYPSSVFQSCLHAAEQGYSLAWYSLGKMY
                     YEGDEVSQDLKLAFNWFTRAAQHNIIDAQYALGIMYSDGRGTDKNISEARKWFLLAAQ
                     NGNASAQYKLARISRFADEPLRNYEEALQWYLSAATQGHDLAQYELGQMYIQGIGVER
                     DEVQAHRWFLQSAEQGYLYAQYHTARLYSESESIPQDQEKALYWFTKAAKNGADGAGD
                     AMYELGKYYLTNNDDPENNAEAIQWLTGAAQRGRIEAIFLLAEMYLYGTKDIAKDENH
                     ALHWYEKAARLGSTEAQHQTAAMYAQGTGTKIDNKQAWMWLTIAGNNNPWIEKDALRC
                     LMSEQDIQDAEQTAANCKRLLRI"
     gene            complement(32460..34160)
                     /locus_tag="OHN27_002052"
     CDS             complement(32460..34160)
                     /locus_tag="OHN27_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCQ2286494.1"
                     /translation="MIIGIDLGTTNSLAAVWRNGQSELIPNALGKFLTPSVVCVDEDG
                     MVLTGEAARDLQLIKPQNCASNFKRMMGTSKTLKLGGREFRAEELSSLILRQLKEDAE
                     NYLGEEITEAVISVPAYFGDMQRKATKAAATMAGLNVERLINEPTAAALAYGLHNKDD
                     EHQFLVFDLGGGTFDVSILELFDNIMEVRASAGDNFLGGEDIVDILIDAYCSRRDLPE
                     NIEWREPTLQRHLRIEAERVKRVLSMRDEATFSVEIEGRRYYWHLTTEKFEFLLQTFF
                     ERISMPLERAIRDAKLNISQLDQVVLVGGTTRMPLIRKLVTRLFGRIPAMHLNPDEVI
                     AQGAAIQAALKARHSELQDVVVTDVCPYTLGVDTSKSLGHTRESGYFAPVIERNRSIP
                     CSRVRAFYTAHDQQTEVNFKIYQGESRMVADNIFLAELSVNVPPKPAGDVRIDVRFTY
                     DINGILDVDISVPLTGAKNSLVIEQNPGALTEEQIQQSLSKLSLLKIHPRDEQINQAF
                     IARLDNLYQFTLAETRDWISNCSRHFSYLLEKQDPDQLADFRAEVEPTLDYLERERLQ
                     "
BASE COUNT         8466 a         8955 c         8303 g         8492 t
ORIGIN      
        1 aaacttaacc attttaaaat aaataaacaa ttaaagaaaa aagatcactt atttatagca
       61 atagatcgtc aaaggcagct ttttgttaca ggtggtttga atgaatgtag caatgaaata
      121 caaaatttca ggccatgtaa ctcccggcaa aaccgggagg tacgtaatcc ttactcagtc
      181 acttcccctt cctggcggat ctgatttgcc caacgctggg cggattcagg cacagtaaac
      241 gccggtgagc gcagaaatga ctctcccatc agtacaaacg caacatattt gccacgcagc
      301 atccagacat cacgaaacga atccatcttt atcgcatgtt ctggcggcgc gggttccgtg
      361 cgtgggacat agctaataat ctggcggttt tgctggcgga gcggtttctt cattactggc
      421 ttcactaaac gcatattaaa aatcagaaaa actgtagttt agccgattta gcccctgtac
      481 gtcccgcttt gcgtgtattt cataacaccg tttccagaat agtctccgaa gcggggtctg
      541 gctggtggtc tatagttaga gagttttttg accaaaacag cggccctttc agtaataaat
      601 taaggagacg agttcaatgt cgcaacaaaa cgaaaagaac ccacatcagc accagtcacc
      661 actacacgat tccagcgaag cgaaaccggg gatggactca ctggcacctg aggacggatc
      721 tcatcgtcca gcggctgaac caacaccgcc aggtgcacaa cctaccgccc ctgggagcct
      781 gaaagcccct gatacacgta acgaaaaact taattctctg gaggacgtac gcaaaggcag
      841 tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc gccgatgatc aaaactcact
      901 gcgtgccggt aaccgtggtc caacgctgct ggaagatttt attctgcgcg agaaaatcac
      961 ccactttgac catgagcgca tcccggaacg tattgttcat gcacgcggat cagcagctca
     1021 cggttatttc cagccatata aaagcttaag cgatatcacc aaagcggatt tcctctcaga
     1081 tccgaacaaa atcaccccag tatttgttag gttctctacc gttcagggtg gcgctggctc
     1141 tgccgatacc gtgcgtgata tccgtggttt tgccaccaag ttctatactg aagagggtat
     1201 ttttgacctc gttggcaata acacgccaat cttctttatc caggatgcgc ataaattccc
     1261 cgattttgtt catgcggtaa aaccagaacc gcactgggca attccacaag ggcaaagtgc
     1321 ccacgatact ttctgggatt atgtttctct gcaacctgaa actctgcaca acgtgatgtg
     1381 ggcgatgtcg gatcgcggca tcccccgcag ttaccgcacc atggaaggct tcggtattca
     1441 caccttccgc ctgattaatg ccgaagggaa ggcaacgttt gtacgtttcc actggaaacc
     1501 actggcaggt aaagcctcac tcgtttggga tgaagcacaa aaactcaccg gacgtgaccc
     1561 ggacttccac cgccgcgagt tgtgggaagc cattgaagca ggcgattttc cggaatacga
     1621 actgggcttc cagttgattc ctgaagaaga tgaattcaag ttcgacttcg atcttctcga
     1681 tccaaccaaa cttatcccgg aagaactggt gcccgttcag cgtgtcggca aaatggtgct
     1741 caatcgcaac ccggataact tctttgccga aaacgaacaa gcagcattcc atccaggtca
     1801 tattgttcct ggtctggact tcaccaacga tccgctgttg caggggcgtt tattctccta
     1861 taccgataca caaatcagtc gtcttggcgg gccaaatttc catgagattc cgattaaccg
     1921 tccgacctgc ccttaccata atttccagcg tgacggcatg catcgcatgg ggatcgacac
     1981 taacccggct aattacgaac cgaactcgat taacgataac tggccgcgcg aaacaccgcc
     2041 ggggccgaaa cgcggcggtt ttgaatcata ccaggagcgc gtggaaggca ataaagttcg
     2101 cgagcgcagc ccatcgtttg gcgaatatta ttcccatccg cgtctgttct ggctaagtca
     2161 gacgccattt gagcagcgcc atattgtcga tggtttcagt tttgagttaa gcaaagtcgt
     2221 tcgtccgtat attcgtgagc gcgttgttga ccagctggcg catattgatc tcactctggc
     2281 ccaggcggtg gcgaaaaatc tcggtatcga actgactgac gaccagctga atatcacccc
     2341 acctccggac gtcaacggtc tgaaaaagga tccatcctta agtttgtacg ccattcctga
     2401 cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat gatgaagtga gatcggcaga
     2461 ccttctggcc attctcaagg cgctgaaggc caaaggcgtt catgccaaac tgctctactc
     2521 ccgaatgggt gaagtgactg cggatgacgg tacggtgttg cctatagccg ctacctttgc
     2581 cggtgcacct tcgctgacgg tcgatgcggt cattgtccct tgcggcaata tcgcggatat
     2641 cgctgacaac ggcgatgcca actactacct gatggaagcc tacaaacacc ttaaaccgat
     2701 tgcgctggcg ggtgacgcgc gcaagtttaa agcaacaatc aaggtcgctg accagggtga
     2761 agaagggatt gtggaagctg acagcgctga cggtagtttt atggatgaac tgctaacgct
     2821 gatggcagca caccgcgtgt ggtcacgcat tcctaagatt gacaaaattc ctgcctgatg
     2881 ggaggcggcg caattgcgcc gcctcaaatg atttacatag tgcgctttgt ttatgccgga
     2941 tgcggcgtga acgccttatc cggcctacaa aactgtgcaa attcaatata ttgcaggaaa
     3001 cacgtaggcc tgataagcga agcgcatcag gcagttttgc gtttgtcagc agtctcaagc
     3061 ggcgcagtta cgccgccttt gtaggaatta atcgccggat gcaaggttca cgccgcatct
     3121 ggcaaacatc ctcacttaca catcccgata actccccaac cgataaccac gctgagcgat
     3181 agcacctttc aacgacgctg atgtcaacac atccagctcc gttaagcgtg gaaaacagta
     3241 agcactctga cggatagtat tatcgataaa cgccggatga cacatcacct ccagcgaacg
     3301 atcaccccga tggcctgcgt catccagcac ttgcaggaac agcgactcac tgatctcttc
     3361 accgtagaat gcactgctga atccctgggt agtgcgcagg ttgaccggca aatcaaacgc
     3421 catctgacga tccgcccgca acgcaatgcc ctgctctgcc gcaaacctgg caacaatcgg
     3481 gaaaatctgc gggaacatat gcacatgatg atggctatca agatgcgtag gtttgcgtcc
     3541 aaatagctca atgaaacgca aatactgact ggcaagctcc tgagtaattt cttccagcgg
     3601 taaagcatct tcttctgcca actgccagat ccattttccc agcacaccat cgcgggttaa
     3661 ccccggcata gctgtcagtg gcttacccat agtaaggaca aagtgcatcc ctatggccag
     3721 actcggttca tcacgactca actgcaccgc atggtcaata gcctgcccat tcaccagcgc
     3781 cgtcgtcgac gtgacaatcc cattgcgaca ggcctcgata atgccgtagt tctgaccttt
     3841 gcttaagcca aaatcatcgg cattaacaat cagtaagcgt tccataatca gcctcggtta
     3901 atgtgctttt ttaagctctg cgatgcagtc ggcaaagttt ggcagccatt tctcgtgcgc
     3961 cagaatcatc tcgcgtgcca gcagctcagc atcgcgatcg gaatgcacca acggactaag
     4021 gtttagcgcc agtaacacat cgttaaattc tccgctaagt gcggcgttgc tggcagcaat
     4081 ctcgaagcct ttaatggtgt gaatcagccc catcacttta tcatcgaaat gcgtaatgcg
     4141 cggatgtggc gtcgcgccat cgcgccccag cttacaggtc atttctaccg cccagtctgc
     4201 cggaatatta tcaatatgcc catgatgcgg gatattaacg taatgttctg cttgcttgtc
     4261 gttgtagata gcgttgatca cttcgcacgc tgcatcagag taataagccc caccgcgctg
     4321 ttccagttct ttcggcttaa ctttcaactc cgggttttta tacagctcaa aaagttgttt
     4381 ctcgactttc tgtactacct gcgctcgtgc gccgcctttg tagtattcgc ccatttcaat
     4441 agccagcatc tctttctgct tgaagtaata cagcagataa gaacatggca gcagattcaa
     4501 cgaacgaatt aagccctcac taaatggcag atcgaaaata ttttttacag aggacgcttt
     4561 taactgccct gacgccacac catcaagcaa ttcggcaaag cgcgacttgc catttaccag
     4621 cacatcctta atgaacacca tatggttgag gccgaacaaa tcgatagata aatcatcgct
     4681 gtctttcagc atcagaacat cgcgaataaa catcttcatg ccgatcggaa tattacacac
     4741 gccgataaag cgtttaaatc cggtatgacg ataaacggct tcagtgacca ttcccgccgg
     4801 gttagtgaag ttaatcaccc atgcattcgg acaaagttct tcgacatctt ttacgatgtc
     4861 aaaaatcacc ggaatggtac gcagaccttt aaacaaaccg cccgcgccgt tggtttcctg
     4921 accaagataa ccatgactta atggaatacg ttcatccagt tcacgcgccg gtaattggcc
     4981 aacgcgcagt tgggtagtaa cgaaatcagc atctttcaat gcttcgcggc gatccagcgt
     5041 tttataaagc ttcatcggga cgccagcgtt atcaatcatc cgttggcaga gatcgaaaat
     5101 aatatccagt ttcgctttac caccttcgac atccaccagc cataattcgc tgaccggcaa
     5161 ttcgtgataa cgcttaataa atccttccag taactccggg gtatagctgc tcccgccacc
     5221 aatagtgacg acttttaatt tctggctcat aatttctccc ttcagtacag aatactgata
     5281 tctggcatat ctgccccccc ggacataaat aatccagcaa caggacagat atgtgaattg
     5341 tcaggtataa cgacttactg attaaattca gtcaatttct tacgatagct cttaggcgta
     5401 aaggacgtta atttcttaaa cgttttaata aacaaactcg ggctactata accggcctca
     5461 aacgcaatat ccgtcactga atagttggtc atttccagtt gttttttggc aaaattaata
     5521 cggatttcat taataatctg cattggcgtt ttgccataat atcgttgagt cgctcgcgtc
     5581 aaatattcct gtgatttggc tgacaacgtc accatattct ccagcgccga ttcactaaac
     5641 tgctctttat catgcatctt ttctaccgta cttttcagcc actgcggtac atcatcaatc
     5701 acctgttctt cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac catctcaaca
     5761 aactcttcca gccctgtttc gcggaaattc aatgaagaaa tcactgtttc cacataggtg
     5821 agaaaagcgt tattggtccg gtatacctgc gaagcgacaa agcaataagg caacaatggc
     5881 aggtaatgct gctcaaaaaa gcgtttactg atcccaacgt tcaatatgcg cgtggcacca
     5941 aactcataaa aactttgatg gtgcgaacct aacggaataa aaacaaaatc gccccgttcc
     6001 agtaacacgc gcttaccgtt aatctcctgg aaataacgcc cggttaatac cagagtaaat
     6061 tcataatagt cgtgctgatg cagtccgctg atactctcag ttttgttata gataaacaca
     6121 tggaagtttt tgccattaaa caactgctgt tctcgggcag tggcaatttc cggcgcgtta
     6181 atcactggct gcatcatcga ctccttatgc cttcagtttt tcatgaagct caattaattc
     6241 agtaatcagt tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt ggaccagcac
     6301 cagactcact ttcatcttac cttcgcccgc atcgccttca atcagtttcg tctgtaccag
     6361 atgcgcttca ttcaatgcca tccgtgactg atccatcatg gcttttgctg cggcaaaatc
     6421 gccctgcttc gcctgtttca gtgccgcata cgccaggctg cgcgcttgtc cggagttgat
     6481 gatcagcccc atcaccactt cttccagctc ttcagcttcc gtttgcgtat cgggaatgtt
     6541 atcgagatcc atcatacatc gttcctctct tttcttaccg gcacgattac ccgtaccggc
     6601 atcgattaaa atttcagggc gttagcgata tcttcttcgc tctcttcttt atcaatcgca
     6661 ttttgtgctt tgttagccac cacaacaaag ggcagataaa ttaacgttgc gatgccaagg
     6721 ttgaagagtg cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa ggctcccaga
     6781 ccggttggca tggtccacgg tgcaatattg gtcaccggag gaataatgcc catgtagtac
     6841 gctgcgaggg tgattgccgc cagaatcggt tgtaccagta caaacgggat aaacatcacc
     6901 gggttcatga taattggcag accaaacaga atcggttcgt taatctggaa gatgccggac
     6961 ggcagcgcca gttttgccac ctgacgataa tcagcacggc gagaggcgat aaagatagcc
     7021 aggatcaggc ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc cagcatcggc
     7081 ttggcccaga tatggaaggt cttaccggct gccagcgccg cttcgacgga accatattgc
     7141 tgataggtcg cgatattttc cagtgcccac ggcgtcataa tgccgttgtc cagtgcggtc
     7201 agcgccagcg cgccatgaat accgaagaac cagagcagtg gaacaaagat cacataggcc
     7261 cagcccacca cgctacccaa cgatgccagt ggggttgaga tggtatccat aatgatctga
     7321 tggaagttgg tgccccaggt attcaacgcc caggcaataa tccccatcac ggaaagaata
     7381 ataaagccgg gaattaatgc cgagaaggaa cgcgatactg aagcaggtac gctgtcgggc
     7441 agtttaatga cccaattgcg gcggacaata aaggtaaaca tttctgccac caccaggcca
     7501 ataataatcc cggagatgat attcgcccca cctaaccagt ttgcaccaac cgcataggcc
     7561 tcaccgacac tatatggggt gacggtcata aatgctgcaa cggataacaa cccagccgcc
     7621 agcgcatcga ctttacgctc ttctgccagc gccatgccaa taaagaacgg tgccattaaa
     7681 gacattattc ctaatgttcc gttatatacg ttgccgccaa tacctttcag accattaagt
     7741 gtttcaatgg ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga cccctcccca
     7801 aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt taacggcatt
     7861 aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat tttaactgca
     7921 aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa atacccttaa
     7981 aaaccgcaat ttaaatattg cggtattgat ttatgaaata actctttgac gggaaaattt
     8041 aaaataaatt aatttgctgc ggctttttta atcgctgcaa ccgcagcctt aagcacgcct
     8101 aaaccatcga ctttgccata aagcagcgag tcaattactt caaccggttt gttgggtaac
     8161 aaacgctgga tttcgggcaa catataagca atctgcggcc ctaataacac gacatcggca
     8221 ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt caataatgac cggaacttca
     8281 tatttttctg cctgtgcgcg catttttgat accagtaaag aggtagacat gcccgcagaa
     8341 caaaacagat aaatgtgttt cttttccata aaactgccct catcgacgat tatctgtcag
     8401 ccagacactc cgcaagcctt aacctgcttc catgctctgg gtaacttgcg aaaccaacat
     8461 gacttttttt gtggctgaaa ggagtatacg gtatagacct gagcaatagt atttctttga
     8521 ccttataaat tgtttctcct tgacctgtgg ttatgaccct cttcacattt cgggcaaata
     8581 attcgcgaag ataattaaga actcaggcca aaaaaaaccg gcgcaatggc cggtttccgt
     8641 tgttactcaa gctttcagac gaattgatta cttcgcagcc tgtggatcag tgtcgtattc
     8701 agcacaggtc tgataaccgg agttgatgac atgtccggta tcatctaacg cgacaaagta
     8761 ggtttctgct ttaccatcac gttgacccag gatgtaggtc tggcaagtac cgcgagcatg
     8821 gatcatgctc acttcagacg aaggtttacc cgcaatttgt gcaacctgcg cccggctcat
     8881 gccttttttg acgtctttca ccacaggctg tacaaactgg tctttggtac gatcataagc
     8941 cgtacaaccc gccagcatgg ttaataccgc cgctgcactc agaattcctg ccatattctt
     9001 gttcatattc cgtcctcttg tttatcagcg tgttagataa gcctggaata cattgggcgc
     9061 tttttcaagc ccgtgaacga aacggctccg ctttcagagg attcctgtat gacgttttaa
     9121 ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct ttaaggaagt
     9181 tttgtctttt ctgtctggag gggttcaatg acattgcaac aacaaataat aaaggcgctg
     9241 ggcgcaaaac cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga ttttctgaaa
     9301 agctacctgc aaacttatcc gttcattaaa tcactggtgc tcgggatcag cggcggtcag
     9361 gactccacgc ttgccggaaa gctgtgccag atggcgatta atgagctgcg cctggaaacc
     9421 ggcaacgaat cactgcaatt tattgccgta cgcctgccct atggtgttca ggccgacgaa
     9481 caagattgcc aggatgccat tgcctttatt caaccggatc gcgtattaac cgttaatatc
     9541 aagggcgcgg tattggccag cgaacaggca ttgcgggaag caggcattga actgagcgat
     9601 tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag cattgcgggt
     9661 atgaccagcg gtgtcgtggt gggcaccgat catgcagcag aagccattac cggattcttc
     9721 actaaatatg gtgacggcgg tacggacatt aatccgctgt atcgtctcaa caaacgtcag
     9781 ggtaaacagt tactggcggc attagcttgc ccggaacacc tttataagaa agcgccaacg
     9841 gccgatctgg aagatgatcg cccttctctg ccagatgaag tggcactcgg cgtgacctat
     9901 gacaatatcg acgactatct ggaagggaaa aacgtacctc aacaggtcgc cagaacaata
     9961 gagaactggt atctgaaaac cgaacataaa cgccgtccgc caattaccgt tttcgatgat
    10021 ttctggaaaa agtaataatt tgcacatatt ggattgtgcg aaaaagagta atttgttcac
    10081 gccggatgcg gcgtgaacgc cttattcgac ctataaaact atgcaaattc aatacattgc
    10141 aggagtcgaa taggcctgac aggcgtagca cgtcagacgg tgtaaccttt gtcatcgacc
    10201 cgcctctttt ttaatcgctt cccgcctgtt acactggata gataaccagc attcggagtc
    10261 aacagtggta cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg caatttatga
    10321 atatcccgaa catttacgtt cattccttaa tgacttaccc acccgaccag gggtgtatct
    10381 gtttcatggt gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta acatccgcag
    10441 ccgcgtcctt tctcatttac gtaccccgga tgaagccgcc atgctacggc aatcccgacg
    10501 gatcagctgg atatgtaccg ccggtgaaat cggcgctctg ctccttgaag cgcgattaat
    10561 caaagaacaa cagccgctgt ttaataaacg gttgcgccgc aatcgccagc tctgtgccct
    10621 gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt tttcacgagc
    10681 ccccaacctg tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat tgcagaccat
    10741 cgctgatgaa caaaaacttt gttatggcct gctgggactg gaaccgttaa gtcgcggtcg
    10801 tgcatgtttt cgttcagcgc taaaacgttg cgccggagca tgctgcggta aagagagcca
    10861 tgaggaacat gcgctacgct tgcgccagtc tctggagcgt ttgcgggtgg tgtgttggcc
    10921 ttggcaaggg gcggtggcgc tgaaagaaca gcacccggaa atgactcaat atcatattat
    10981 tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa cgttaattcg
    11041 gacacccgcc gggtttgatc acgacggtta taaaattctt tgtaagccgc tgctttccgg
    11101 taactatgaa attactgaac ttgatccggc gaatgaccag cgagccagtt gatttcactg
    11161 aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc gaaccagcac
    11221 gcgccctggt cggtagtgag caatttatcg cccagttgcc acgccccgtt aatcacaaac
    11281 accaccccgc cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct gactttcgcc
    11341 ttacagacat ccaggcgtgt catgatgttg aaatccatcg acatctgccc tgccgtcagt
    11401 ttcgctttta ccacctggtc cgctgcaaag gcaaaaggct ggaacggttt taaggtatgg
    11461 ttaaagcggt ctgcgctttc aaggagcatc tcgccgcctt ccagcaacgt cactatcctt
    11521 tccatgccgg gaaacaaaga aaactcccca ttcgccgcga tggatgcaat gctggcacgc
    11581 cagtaaaaat cacgttttgc cggtgggaac gtgcaaattt cgcgcgtttc accggcagca
    11641 tttcgccaca gattcaccga cattttacgc atatcaaagt attccatact cgctcccggc
    11701 ccgttctttt cattgttatc gttcaggtaa ccgtggatgg ttatcggcaa ctcgggtgat
    11761 ttacgttagt ggtgatcagg ctttttttac aactgtttga gaagagaaaa gaaaaccgcc
    11821 gatcctgtcc accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg
    11881 gctgaaagat tattcagcag ttgcaggcat tttacctttt gccgctggac gttctgtcag
    11941 acgcttctca aaattagcat taaattgctt tttctgttcc ggcgtcagga tgttgtaaat
    12001 tttgttctgg gtttccatgt gcgccagcat gttagctttg cgctgttctt ccatttttgc
    12061 gatctgcgct tcagctttta ctttatcgaa ggtatcgctg gcaatgatgt catgcattgc
    12121 gcggcgttct tccagcggcg gacgtttcat ctggtcacgc tggcctttca tgatttcgcg
    12181 gatctgctgt ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt cctgatgcgg
    12241 accgaacttg cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg cggtagtggt
    12301 gtctgcggca tgggccaggt tagccgcgcc aagagccagg gtagaggcaa caaacagtgc
    12361 agttaattta cgcatattct atatccttcc tttcagttat ttattacggc tttcttaagt
    12421 agcgtgccgt gttgacgaga ttaactttac tgactttagc gtcaattaat cagagcaacg
    12481 gtaaaacaat gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa aaagaaaatt
    12541 tgatggagag tgatgagaga atattacaac aagatgattt tgcagagatt atgaagaact
    12601 ataccggatg actggtgata aataaagcaa ataaccagga ttaatctgta ttaatttata
    12661 agaaagcaac ttaatacccg cagaatgatt tctgcgggta agtattagct tattttttcg
    12721 agcattaatc ccgcgcgtaa tcccaacgct accaacggat tagggaataa cacatactct
    12781 acatcatggg ttacggtaaa acgttcctct ccgtcctgcg ccagcaatgt tcctttctca
    12841 aacggcataa aattcagcgt gtcacttgcc atatgcattt cgaaggacgg cgagtggcga
    12901 gtaatttgcg aaaccacccg ataacggagc ggcggtgttc tcacgatacc gacactctca
    12961 ccagatagca gcgcagcaat tgcgctggca gttactgcaa actggcgaag atcgttttgc
    13021 ccaaagggca acgctttgcc aagttccagc gtacaggcca gcgcgccaaa atgtctggcg
    13081 ctgaaatggg taaacgtacc accaggttcc tgatggaaca ccagcgcctc cagccccgcc
    13141 gcacccagcc acgtcagaaa tttctcgtcc cagggaatgt cgcgttgcgg taatacaccg
    13201 aactgcggat gcaaggagcc acgaattgcg gtatgtagat caaggtgcca gcgcacagat
    13261 tctttgccct ggtcataaaa atcttccagg cactgttcca gttcgcgcgc ccgacaggtt
    13321 tctccgcttt cagcaaatag ctgccaacga ccgccaaaca ttcgattcat atcgctatgg
    13381 caataacgtt tcccttgctt cagcgcagga ggattcccga ggatcaccag caaccgccaa
    13441 cgtaacggga tctcgccgtg agatatcgcg ccaagcaacg cgtccagcat ctccacaggt
    13501 gccgtctcat taccgtgtat tcccgctgaa atcaccagtg cgccttgcgg tggcgttaat
    13561 ggcgtcagtt ccagcacacc atcgcccagc cagcgccagc gaacgccgtt gatttctcgc
    13621 tcggtgataa ccggtttttt acccgttaag gtcagagcaa gaaaattatc cattgccccc
    13681 tccctcgcgc tggaacggat aaaccgaacc gagattcagt aattggctca atacatccag
    13741 tgcttcccgc ccttcgcgca gcaattgcgg gtcggccaga tcggcagcag taaggcgatc
    13801 gcggtagtaa cgatccaccc agtcattgag cgcattaaac agcgtatcgt tcatcatcac
    13861 cgccggattc accgcccggc gttcttcttc tgtcaatacc acccgcaacc gcaggcacgc
    13921 cgggccgccg ccattcgcca tgctttcacg taaatcaaag acttttagtt cgctaatcgg
    13981 gttgtcagcg gcaaggagtt cattgagata accccatact ccggcgtgtt cccgacactc
    14041 ctgaggcagc accaacatca tggaaccatc atcgcggctc agcagttggc tgttaaacag
    14101 ataggtagac accgtatcag acacggaaac ctgagttgcc ggaacttcta tcgccataaa
    14161 accattgacc cgcgcacgca ggtttgccag taactgtgac tggcgagcga acgcctgttg
    14221 gtggcaaaac agcacctggc ggttactcac ggcaatcacg tcattatgaa aaacgccctg
    14281 gtcgataacg tccgggtttt gctgggcgaa aatcacctgt tggggattca cctgattcag
    14341 ccttgccacc gcctcgctgg cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt
    14401 atcattgcct tcttctcgcc cgtagacaaa aagttgcata cccggttcac cgtaatgacc
    14461 gccgagacga ttgtggtttg ccgccccctc atcaccgagc aacgctacct gtggcaacgc
    14521 cgaatggacg ctaaatttct cttcgtcgtt aaaaatcgct tttaacagcg attcagtgac
    14581 gggcgcttcc agcgaacggt gaaatttatt gttcaggttg gcaacggtga gatgcacttt
    14641 gccatccagc gtatcggcag atggcgcgat cgttgccgca ttggctaccc acattggcga
    14701 agcggaactg acgctggaaa gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg
    14761 ctcatcgcta ccactgaatc ccaactgacg cagcaccgga ataaacggac gctcgtgcgg
    14821 cgggatcacg gcctggggga atcccgcatc ggcaagggct ttcattttca gtaagccctg
    14881 ttttgccgcc tgtcgcgggt tagacacctg aaaacggtga cgggtagagg cttcattacc
    14941 aaacgacagg ccagcgtaat gatgcgtcag ccctaccagc ccgtcgaaat taacttccca
    15001 ggcgttcatc gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt tgcgggcaaa
    15061 gttaacgagt ccgactccag gctcgccata ggccatgcgc agtaatctgc ggcataccag
    15121 gcgctggggc gatggttacc ggatgcacca atgccgccga atggcgcggt actggcagca
    15181 ccagtaagcg gtttgttcca gttaacaatc cccgcccgcg cctccagcaa cagttgatcg
    15241 aatttttccc gctcggggga aaccagaccg caagagagtc cgaagcgagt gttattcgcc
    15301 attagaatcg cttcctcgaa agaatcataa cgccagacgc gcaaaagcgg cccgaacacc
    15361 tcttcatctg gtacgccagc aacgcctgtc atttcaatga tccccggcgt cagcaacgat
    15421 gtctctgatt gtaataagcg cggcgcaagc agggttcgtc cgcccatcgc ttccagttgc
    15481 tgccaggcag taaccacctg ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc
    15541 tgcggttcgt catcccagtt gcccggcgtt aatcgctggc tgacggcaac cagacgagca
    15601 agaaacgcat cgccctgcgc tccgcttttg agcaataaac ggcgggcgca ggtgcagcgt
    15661 tgcccggctg tgacaaacgc cgactgaatg gtcagatgga cagccgcgtc gatatccgcc
    15721 acctcatcga taattagcgg attattaccg cccatctcaa gggcgagaat tttctccggc
    15781 tgaccggaga gctggcgatg cagctggtag ccggtattgg cgctaccggt aaacagcaaa
    15841 ccgtcgagat cctccagcgc actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg
    15901 ttcagtacgc ccggcggcaa gccagcctgc tgccataaac gcattaccgc ttcgccactc
    15961 cacggtgtca gttcgctggg tttaaagata atggtgttac ctgccagcaa tgccggaacg
    16021 atatgtccgt tcggcaaatg accagggaaa ttatacggcc caaacaccgc cagcacgccg
    16081 tgcgggcgat gtcgcaggct cgccgcaccg tccggcattt cactacgctg ctcgccggta
    16141 cgaacgtgat acgccttaat tgatatcgcg attttattga tcatcgccgt cacttcggtt
    16201 gccgcttccc agcgcggctt acccgtttct ctggcaataa tcgcggttaa ttcggcttta
    16261 ttgctttcca gcaatccggc aaagcgttcg acaacgacct gacgttcagc caatgagagt
    16321 cgcgcccagc gcggaaacgc cgcacgggct gcccgacaag cctgttcgac ctgagcggca
    16381 ccggcatcat tgccttgcca taacacctcg cccgataccg gattacgctt cacacgcgat
    16441 gcgccctggc ccgttatcca gtcaccgtta atccataaag tcatgctgtt ttctcctctg
    16501 cgcacaggcg caccagacga acgcgatccc ctgcgtggca tttgagggca tccagttgtg
    16561 cggcggttaa aatcaaacgc tcggttgccg gatcggtacg caccagcacc acgcggaaat
    16621 ggtgataatt ttcattggcg accaggcagg ccggaaaatc gccctgcgca ggctgccctt
    16681 ctgccacttc caccagccga cttttacgga tggcgcgcac gcggtcgatg tcacactcaa
    16741 gcgttggccc gccgtcgaag atgtcgatat agttgcggta acgaaaacct tctttctcca
    16801 gcaccgcgcg ggcaggcgcg gtttgcggat gtacctgacc gatgacgtcc tgggcttcct
    16861 gggataaaaa gtgggtatag atcggatgtt tcggcatcag ttctgcaata aatgcctttt
    16921 gcccggtgcc gcagagaaaa tcggcgcggc taaaatccat cgaaaagaag cgtttaccga
    16981 ggctttgcca gaacggtgaa tagccgtgtt cgtcaatcac cccgcgcatt tcagcaacca
    17041 ctttgtcatt aaacttgtcg cgaaaagccg ccataaacat aaagcgcgat ttcgacagca
    17101 aatagccgtt gccctctttg cgccagtccg ggtcgagaaa cagcgtgcac agctcgctgc
    17161 tgccggtgtg atcgttactg agaaacagcg tcggcaatgc gttatagaca ttcagctctt
    17221 ttgaggcgtg aaccaacgtg ccgacgcgat agttgtacca gggatcgttc agcccaaccg
    17281 ccacctcaat ggcacaaatc cccgccacgg tgcctgtctc gctatcttcc agcacgaaca
    17341 catagccctg ctcacttttg ggcagttcgc cttgccaggt tttgattgcc ctttcgatac
    17401 gcgccgaaag cgtggcttca ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg
    17461 caagctgcat cagcgccgag acatctgatc gctcaacggg acggatgacc atcatgatga
    17521 acctcggcta acaaagtgtt cgcaagcagc tgcaaagcga tccagtccgg tcgtcacctc
    17581 ttcttcgctg acattgagcg caggcgcaaa acgcaccacg ttgccacctg caatcagtac
    17641 catcacgcct gctttcgccg cttcctgaga gatctgtttc gcttgcccgg cgtaatcggc
    17701 attcagcaca cagccaatca acaaacctaa gccgcgaatt tcgctgaaca aaccgtagcg
    17761 gtgattaagg gtattaagac gctcaacaaa ccagtcgtga cgctgtttaa cgccattaag
    17821 catctctggt gtgttgatga gctccagcac tttgcctgcc accgccgagg ccagcggatt
    17881 accgccatag gtggtgccat gagtgccaac ggtcatcacg cgggcgcact cttcggttgc
    17941 caacaacgca ccgaccggga aaccgccgcc cagcgctttg gcggtagtta acagatcagg
    18001 cgtcacgccg tagtgcatat aggcatacag ttccccggtg cgcccgacgc cggtttgtac
    18061 ttcatcaaaa atcaacagcg cattgtggcg gttacacaat tcacgcagac cttgtaaaaa
    18121 cgcgttgctg gctggcacca caccgccttc cccctggatg ggttcgacaa tcaccgcaca
    18181 ggtagagtcg tcaatcagcg cgctggcaga gttaatatcg ttatatgcag catgacgaat
    18241 atccgccggc agtggcgcaa aatcctgtga ataggctggc tgcccacccg cactgacagt
    18301 aaacagcgtg cgaccatgaa acgcattttt gaacgccacg atgccgctct tatggctgcc
    18361 gtagcggtcg tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc
    18421 ggagttacaa aagaagacgc gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg
    18481 cagtaccggc tcgttggtgt aaccgttgcc ggtatgccag aacttactcg cctgttcgtt
    18541 cagcgcttca cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc
    18601 gaagtcgata tactctttcc cctgctgatc ccacaagcgc gaaccttcgc cacgtaccgg
    18661 tataaagggt gccggagcgt aaacaggtat catccattca tcaaagtttt cacgcgtaat
    18721 tggctgagac atagcgacct ctacagtaaa taattcgtta tttatatgtt aataataagt
    18781 aatgtttgcg ctgtaaatgt agattgcagg gttcgtgcca gccagtgata aaagtgcata
    18841 aacggcggag gctaactgga aatcaaggag ttataaccaa accatatgca tttaaagtgc
    18901 atataaagtg aatacgtttg cgatgtgggt gaataaaaag aataaaaaac gcaatgttat
    18961 gcagaagtaa aatataattc tggaattgtg atcattgacg aaatttactg gaaattactg
    19021 cgccattctg acgcagcgcg caccaaaagc gggcattttt tgcgccatcg ttgacatcat
    19081 taacaaccat cgatcaaatc acttaacaac aggcggtaag caacgcgaaa ttctgctacc
    19141 atccacgcac tctttatctg aataaatggc agcgactatg aaatttgtct cttttaatat
    19201 caacggcctg cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc accaaccgga
    19261 tgtgattggc ctgcaggaga caaaagttca tgacgatatg tttccgctcg aagaggtggc
    19321 gaagctcggc tacaacgtgt tttatcacgg gcagaaaggc cattatggcg tggcgctgct
    19381 gaccaaagag acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg aagaggcgca
    19441 gcggcggatt attatggcgg aaatcccctc actgctgggt aatgtcaccg tgatcaacgg
    19501 ttacttcccg cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa aagcgcagtt
    19561 ttatcagaat ctgcaaaact acctggaaac cgaactcaaa cgtgataatc cggtactgat
    19621 tatgggcgat atgaatatca gccctacaga tctggatatc ggcattggcg aagaaaaccg
    19681 taagcgctgg ctgcgtaccg gtaaatgctc tttcctgccg gaagagcgcg aatggatgga
    19741 caggctgatg agctgggggt tggtcgatac cttccgccat gcgaatccgc aaacagcaga
    19801 tcgtttctca tggtttgatt accgctcaaa aggttttgac gataaccgtg gtctgcgcat
    19861 cgacctgctg ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg gcatcgacta
    19921 tgaaatccgc agcatggaaa aaccgtccga tcacgccccc gtctgggcga ccttccgccg
    19981 ctaatttagc agctctcctg gctcaaactg ggtcaggaga attaaccttg agaaaaatca
    20041 acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt tataccgtct ctgcgttttt
    20101 agttgtctga ccacttctct attatcaagt ttgatatagg aaactccacg atgaacgctg
    20161 agcgtaaatt tctttttgcc tgtcttattt ttgcgctggt catttacgct atccacgctt
    20221 tcggtttatt cgatctgctc accgatttac cccacttaca gacactcatc cgccagagcg
    20281 gatttttcgg ctatagcctc tatattctgt tattcatcat tgccaccctc ttgctgttac
    20341 caggaagcat actggtgatc gcgggaggaa tagtttttgg cccgctctta gggacactac
    20401 tctcattaat tgccgccacg ctggcctcct cgtgctcatt cctgctggcg cgctggctgg
    20461 gacgtgattt actgctgaaa tacgttggcc atagcaatac cttccaggcc attgaaaaag
    20521 gcattgcgcg taacggtatt gattttctta ttctgacccg cttaatcccg ttgtttcctt
    20581 acaatattca aaattacgct tacggattaa ccacaatcgc cttctggcct tataccctta
    20641 tttcggcact cacgacccta cccggtattg ttatttatac cgtgatggca agcgatctcg
    20701 ccaatgaagg cattacgctg cgctttattt tacaactctg tctggcgggc ctggcgctgt
    20761 ttattctcgt ccagctcgca aaactctacg cccgacacaa acatgtggat ctgtctgctt
    20821 cgcgccgcag cccacttact cacccaaaaa atgaaggata gaacgatgtt gcaacattat
    20881 tcagtgtcat ggaaaaaagg actggctgca ctctgtttac tggctgttgc tgggcttagc
    20941 ggctgcgatc aacaagagaa tgcggcggca aaagtggaat acgacggact ttcgaacagc
    21001 caaccgctgc gtgtcgatgc caataaccat acggtaacca tgctggtgca aattaatggt
    21061 cgtttcctca ccgacgacac tcgtcacggt attgtgttta aagatggctc caacggacat
    21121 aaatcgctgt ttatgggtta tgcgaccccg aaagcatttt atgaagccct gaaagaggca
    21181 ggtggtacgc cgggcgaaaa catgacgatg gataataaag aaacgactca tgtcacaggc
    21241 agcaaactgg atatttcggt caactggcaa ggggcggcaa aagcgtattc cttcgatgaa
    21301 gtgattgttg acagtaatgg caagaaactg gacatgcgct ttggcggtaa tttaacggca
    21361 gcagaagaga agaaaacggg ttgcctggtg tgtctggata gctgcccggt cggcatcgtc
    21421 agcaatgcaa catacactta tggtgcggtt gaaaaacgtg gtgaagttaa attcaaaggc
    21481 aatgcctcag ttctcccggc ggataacacg ctggcaacgg ttacctttaa aatcgccgaa
    21541 taaagccagg ataaaggatg atgatgatgc aatcgcgaaa aatctggtac taccgtataa
    21601 ccctcatcat cctgttgttc gctatgctgc tggcatgggc gctgcttccc ggcgtccatg
    21661 agtttatcaa tcgcagcgtt gcggcgtttg ccgccgtgga ccaacagggt atagaacgct
    21721 ttattcagtc ttacggcgca ctggcggcag ttgtctcgtt cttgttgatg attttgcagg
    21781 ccattgctgc accgctacct gcgtttttga tcacctttgc caatgcgtcg ctgtttggcg
    21841 cgttctgggg cggcttgctg tcgtggacca gttcgatggc cggcgcggcg ctgtgctttt
    21901 ttatcgccag agtgatgggc cgcgaagtgg tggaaaaatt aaccggcaaa accgtgcttg
    21961 acagtatgga cggctttttc actcgctacg gcaaacacac cattctggtc tgtcggttat
    22021 tgccttttgt ccctttcgat ccaatcagct atgctgccgg tttgacttca atacgttttc
    22081 gctcgttttt tatcgccacc gggcttggtc agttaccggc aactattgtt tattcctggg
    22141 cgggcagcat gttaacaggc ggtactttct ggtttgtcac cggactgttt attctgtttg
    22201 ccctgaccgt ggtgattttt atggcgaaga aaatatggct tgaacgccag aagaggaatg
    22261 cctgatgggt ttaccgccgc ttagcaaaat tcctttaatt ttacgtccac aggcgtggct
    22321 gcatcatcgc cattacggcg aggtgctaag ccccattcgc tggtgggggc ggatcccgtt
    22381 tatcttttat ctggtgtcga tgtttgttgg ctggctggag cgcaaacgct caccgctcga
    22441 tccggtagta cgatcgcttg tcagcgcgcg cattgcgcaa atgtgcctgt gtgagttttg
    22501 tgtggatatc accagtatga aagtcgccga gcgcaccggc agcagcgata aactgctggc
    22561 agtggctgac tggcggcaaa gcccgctctt tagcgatgaa gaacggctgg cgctggagta
    22621 cgccgaagcc gcaagcgtaa cgccgccaac ggtcgatgat gccctgcgta cccgactggc
    22681 tgcgcatttt gacgctcagg cgctcaccga actgacggca ttgatcggcc tgcaaaatct
    22741 gtcagcccgt tttaattctg ccatggacat tcccgctcag gggctgtgcc gtattcctga
    22801 aaaacgttct taaggagaga tgatgcgcca ttgtgggtgg ttgctgggat tgttatcgct
    22861 gttttctctg gcaacacatg ccagtgactg gcaagaaatt aaaaatgagg ccaaagggca
    22921 aaccgtctgg tttaacgcct ggggcggcga taccgcaatt aaccgctatc tcgactgggt
    22981 gagcggcgag atgaaaaccc attacgctat aaacctgaag attgtccgtc tggcggatgc
    23041 cgcagacgcg gtgaagcgca ttcagaccga agccgcagcc ggacgtaaaa cgggcggctc
    23101 ggtggatctg ctctgggtga acggcgaaaa cttccgcacc ttaaaagagg ccaatttatt
    23161 acaaacgggc tgggcggaga ctctgcccaa ctggcgctat gtcgacacac agctgccggt
    23221 gcgggaagat ttttctgtgc cgacacaagg tgcggaatcg ccctggggcg gcgcacaact
    23281 gacgtttatc gcccgccgcg atgttacgcc acagccacca caaacgccgc aagccttact
    23341 ggagtttgct aaagccaatc ccggcacggt tacctatccg cgcccaccgg actttaccgg
    23401 cacggcgttt cttgaacagt tgctgattat gctgacgccc gatcccgccg cattaaaaga
    23461 agcgccggac gatgcgactt tcgcccgtgt cactgctccc ttgtggcaat atcttgatgt
    23521 gctgcatccg tatttgtggc gcgaaggaaa ggatttcccg ccttcacccg cgcggatgga
    23581 tgctctgctg aaagccggca cattgcgcct gtcgctgacc tttaaccccg cgcatgcgca
    23641 gcaaaaaatc gccagcggcg atttgcctgc aagcagttac agttttggct ttcgcgaggg
    23701 gatgattggc aacgtgcatt tcgtcaccat tcctgccaac gcgaatgcca gtgctgcggc
    23761 gaaggtagtt gccaatttcc tgctctcacc cgatgcgcaa ctgcgtaaag cagatcccgc
    23821 tgtctggggc gatccttctg ttctcgatcc gcaaaaactg cctgacgggc agcgcgaatc
    23881 attgcaatca agaatgccgc aggatctgcc gccggtactg gctgaaccgc acgcaggttg
    23941 ggtaaatgcg ctggaacaag aatggctaca ccgttacggt acgcattaat ctttttgctg
    24001 tgggcgatgg tggcggtgat ttatgcaccg ctgatcccgg cagctctcac gctgatatcg
    24061 cctgctttgt cgttgacaca ctggcaggcg ttatttgccg atccacagtt accgcaggca
    24121 ttactggcaa cgctggtgtc gacaaccatc gcggcggtcg gggcattgtt gattgccctg
    24181 ctggtgattg tggcgctgtg gcctgggccg aaatggcagc gtatgtgcgc ccgtctgccg
    24241 tggctgctcg ccattcccca tgtggctttt gccaccagcg cccttctgct ctttgctgac
    24301 ggagggctgc tttatgacta tttcccgtat ttcactccgc caatggaccg atttggcatc
    24361 ggtctgggcc tcacccttgc agtgaaagaa agcgcatttc tgctgtggat cttagcagca
    24421 gtattgagcg aaaaatggct gttgcagcag gtcattgtgc tggattcgct gggctacagc
    24481 cgctggcaat gcctgaactg gctgctgttg ccctccgtcg cgcctgcgct ggcaatggcg
    24541 atgctggcga ttgttgcctg gtcgctgtcg gtcgtggatg tggcaattat tctcgggcca
    24601 ggtaatcccc cgacgctggc ggtaattagc tggcagtggt taacccaggg cgacattgac
    24661 caacaaacaa aaggcgcgct tgccagcctg ctgttgatgc tgttactcgc cgcctacgtt
    24721 ttgctgagct atctgctatg gcgcagctgg cggcgcacta ttccccgcgt agatggcgtt
    24781 cgcaagcctg ccacgccttt attgccgggc aatacgctgg cgattttttt acccttaacc
    24841 ggtgtgctgt gtgtggttct gctggcgatc ctcgcggatc agtcgacgat caatagtgaa
    24901 gcgctcatca acagcctgac aatggggctg gtggcgacat tcatcgcttt gctcctgtta
    24961 ctgctgtggc tggaatgggg gccacagcgt cgccagttgt ggctatggtt gcccatttta
    25021 ttacccgctc tgccactggt ggcgggccag tacacgctgg cgctatggct gaacctggat
    25081 ggaagctgga cggcggtggt ctgggggcat ctgctgtggg taatgccatg gatgctgttt
    25141 atcctgcaac ccgcctggca gcgcattgat tcacggttaa ttttgattgc gcaaacactg
    25201 ggctggtcgc gggccaaaat cttcttttac gtgaaatgtc cgcttatgct acgccctacg
    25261 ctgattgcct tcgcggtggg atttgcagtc ggtattgcgc agtatatgcc aacgctgtgg
    25321 ctgggcgcgg ggcgttttcc gacgctcacc actgaggcgg tggcattaag cagcggcggc
    25381 agcaacggta ttctcgccgc ccaggcttta tggcaactgc tattaccgct tattattttt
    25441 gccctgaccg cgttagtcgc aaaatgggta ggttatgtca gacaaggact ccgctaatgc
    25501 tctgcgtgaa aaatgtttcg ctacgtttac cagaaagccg cttgctgaca aacgttaact
    25561 ttacggtgga taaaggtgac attgtcacgt taatggggcc gtctggctgt ggaaaatcca
    25621 ctctgttttc atggatgatt ggtgcactgg ccgaacagtt ttcttgtaca ggtgagctat
    25681 ggctcaatga gcaacggatt gacatcctac ccaccgcaca gcgtcagatt ggcattcttt
    25741 ttcaggatgc actgttattt gaccagttca gtgtcgggca aaatttactg ctggcgctac
    25801 cggcgacact taaagggaat gcccgacgta atgccgtgaa tgatgcactt gagcgttcag
    25861 gccttgaggg agctttccat caggatcctg ccactttgtc tggcggtcag cgagcgcgcg
    25921 ttgctctact acgcgccctt ctcgcccaac caaaagcgtt actcctggat gagccattca
    25981 gccgtcttga tgtggctctg cgcgataatt ttcgccagtg ggtgttcagc gaagttcgcg
    26041 ccctggcgat ccccgtcgtt caggtaacgc acgatctcca ggatgttcct gctgatagtt
    26101 ctgttctgga tatggcgcag tggtcagaaa attacaacaa actgcgataa cgcaaagttt
    26161 ttctcaatgc gtcagttcag aatggcgcac tcaaaactac aatgtcggga ttttcgatga
    26221 aacgtgtttc tcaaatgacc gcgctggcaa tggctttagg gctggcttgc gcttcttcgt
    26281 gggccgctga actggcgaag cctcttacac ttgaccagct tcaacaacaa aatggcaaag
    26341 cgatagatac tcgccccagc gcgttttata acggctggcc acaaacctta aatggccctt
    26401 ctggtcatga acctgccgcc ttaaacctct ctgccagctg gcttgacaaa atgagcaccg
    26461 aacagctcaa cgcgtggatc aagcaacata acctgaaaac cgatgctccg gtggcgctgt
    26521 acggtaatga caaagatgtc gacgccgtca aaacgcgact gcaaaaagca ggtttaacgc
    26581 atatctccat cctgagtgac gcgctaagcg aaccttcccg tctgcaaaaa ctgccgcatt
    26641 ttgagcagct ggtttatccg caatggctgc acgacctgca acaaggtaaa gaggttacgg
    26701 cgaaacctgc cggtgactgg aaagtcattg aagcggcctg gggcgctcct aagctttacc
    26761 ttatcagcca tattcccggc gctgactaca tcgataccaa cgaagtggaa agtgaaccgc
    26821 tgtggaacaa agtttctgat gaacaactaa aagcgatgct ggcaaaacac ggcattcgcc
    26881 atgacaccac ggtcattctg tatgggcgtg acgtatacgc tgcagcgcgt gtggcgcaga
    26941 ttatgcttta tgctggcgtg aaagatgtgc gcctgctgga tggcggctgg caaacctggt
    27001 ccgacgcggg actgcctgtt gagcgcggaa cgccaccgaa agtgaaagcg gaaccggatt
    27061 tcggcgtgaa gatcccggca caaccgcaac tgatgcttga tatggaacag gcgcgtggac
    27121 tgctgcatcg ccaggatgca tcgctggtga gcattcgttc gtggccagaa tttatcggta
    27181 cgaccagcgg ttacagctat attaaaccaa aaggtgaaat agccggagca cgttggggac
    27241 acgctggtag cgactcgacg catatggaag atttccataa cccggatggc accatgcgta
    27301 gcgccgatga tattaccgct atgtggaaag catggaatat caaaccagat cagcaagttt
    27361 cattctactg cggcaccggc tggcgcgcgt ccgaaacctt tatgtacgca cgagcaatgg
    27421 gctggaataa cgtctccgtt tacgacggcg gctggtacga atggagcagc gatccaaaaa
    27481 attcggtagc aaccggtgaa cgcggcccgg acagcagcaa ataacattgc aattactgac
    27541 gctggagcga cttcagcgtc agatacccac tccacacccg cgtaaaggtc gtcatccagc
    27601 acaacgcacc aaatacccac gcaaaccacg gaaaacaggt cggaaagagg caactcagaa
    27661 caaacagcaa aatcgtctcg ctgccttcgg ttaacccgcc cagataataa aacgatttat
    27721 gcgcataacc gggattatca atctgatgtt tcgccgccag cgcagcaaaa gcgagaaaac
    27781 tgctacccgt gccgataaac gcaaacagca gccagccgcc tgccagggcg ttttgctgcg
    27841 gtgcggcgag gataaaccca aaaggcacca gcgcgtaaaa aagaaaatcg agagaaatat
    27901 caagaaagcc gcccgcgtcg gtaagccctc tgcgccgcgc cagcgcaccg tccagaccat
    27961 caagcagcct gttcaacaaa atgacgacta gcgccgccag ataccagccc agcgccagaa
    28021 acggcagagc cagcacgccg atggcaaatc ccactaatgt taaaccatct ggcgtaatgc
    28081 ccggtttatc aagaacccgc acgcactgat gcaacaacgg tttaatccgg ggatgaagat
    28141 ggcggtctag cacaggcact ccttaaatat aaagcctttc tgattgagca acagtgcgga
    28201 tattatggca tttttcgctt atctgcccgt gtgtaattta tgaaaatgat tgaagttgtt
    28261 gccgccatca ttgaacgtga tggcaaaatt ttactcgcgc aacgccccgc ccagcgcgat
    28321 caggcgggat tatgggagtt tgccggtggt aaagtcgggc tggatgaaag ccagcagcag
    28381 gcgctggtgc gtgagttaaa cgaagaattg ggcatcgaag caactgtggg tgagtatgtt
    28441 gccagccatc agcgagaagt ttcggggcgg attatccatc ttcatgcctg gcacgtaccc
    28501 gacttccacg ggacgttaca ggcacatgaa catcaggcgc tggtctggtg ctcacctgaa
    28561 gaggcgctgc aatatccgct ggcccctgct gacattccac tattagaggc gtttatggct
    28621 ttacgcgccg ccagaccagc ggattagtgc taagggtttt gtcatcacgc tggcattgca
    28681 gcagtattcc ctcggcttta attaccgctc cttcagaata atttttatcc tgataaacgc
    28741 agcactgagt acagggctgc gctgactgcc cgcctgaact gaacacttct ggcggtacgt
    28801 ttacctccac gtccggacga taatgcgggt tagccagtgc aattaatgga aatgctaata
    28861 ctacggcgaa caatgctcga ctcacaggga gctccttaac gttattgtct ctgctactga
    28921 taacggtagc cgggcggcaa aactttagcg tctgagttat cgcatttggt tatgagatta
    28981 ctctcgttat taatttgctt tcctgggtca tttttttctt gcttaccgtc acattcttga
    29041 tggtatagtc gaaaactgca aaagcacatg acataaacaa cataagcaca atcgtattaa
    29101 tatataaggg ttttatatct atggatcaga catattctct ggagtcattc ctcaaccatg
    29161 tccaaaagcg cgacccgaat caaaccgagt tcgcgcaagc cgttcgtgaa gtaatgacca
    29221 cactctggcc ttttcttgaa caaaatccaa aatatcgcca gatgtcatta ctggagcgtc
    29281 tggttgaacc ggagcgcgtg atccagtttc gcgtggtatg ggttgatgat cgcaaccagg
    29341 tacaggtcaa ccgtgcatgg cgtgtgcagt tcagctctgc catcggcccg tacaaaggcg
    29401 gtatgcgctt ccatccgtca gttaaccttt ccattctcaa attcctcggc tttgaacaaa
    29461 ccttcaaaaa tgccctgact actctgccga tgggcggtgg taaaggcggc agcgatttcg
    29521 atccgaaagg aaaaagcgaa ggtgaagtga tgcgtttttg ccaggcgctg atgactgaac
    29581 tgtatcgcca cctgggcgcg gataccgacg ttccggcagg tgatatcggg gttggtggtc
    29641 gtgaagtcgg ctttatggcg gggatgatga aaaagctctc caacaatacc gcctgcgtct
    29701 tcaccggtaa gggcctttca tttggcggca gtcttattcg cccggaagct accggctacg
    29761 gtctggttta tttcacagaa gcaatgctaa aacgccacgg tatgggtttt gaagggatgc
    29821 gagtttccgt ttctggctcc ggcaacgtcg cccagtacgc tatcgaaaaa gcgatggaat
    29881 ttggtgctcg tgtgatcact gcgtcagact ccagcggcac tgtagttgat gaaagcggat
    29941 tcacgaaaga gaaactggca cgtcttatcg aaatcaaagc cagccgcgat ggtcgagtgg
    30001 cagattacgc caaagaattt ggtctggtct atctcgaagg ccaacagccg tggtctgtac
    30061 cggttgatat cgccctgcct tgcgccaccc agaacgaact ggatgttgac gccgcgcatc
    30121 agcttatcgc caatggcgtt aaagccgtcg ccgaaggggc aaatatgccg accaccatcg
    30181 aagcgactga actgttccag caggcaggcg tactgtttgc accgggtaaa gcggctaatg
    30241 ctggcggtgt agcaacgtca ggccttgaaa tggcgcaaaa cgctgcgcgc ctgggctgga
    30301 aagccgagaa agttgacgca cgtttgcatc acatcatgct ggatatccac catgcctgtg
    30361 ttgagcatgg tggtgaaggt gagcaaacca actacgtgca gggcgcgaac attgccggtt
    30421 ttgtgaaggt tgccgatgcg atgttaagcc agggcgtgat ttaatcgtta ttataatcgt
    30481 ctggttttcc ttataaccag acgattttcc ctgagttaat taaatcctta gcagacgttt
    30541 gcaatttgca gccgtttgtt ctgcatcctg aatatcctgt tcagacatta agcatcgcaa
    30601 cgcatctttt tcaatccagg ggttgttatt tccggcaatt gttagccaca tccatgcttg
    30661 tttattatca attttagtgc ctgtcccctg agcatacatt gccgcagtct ggtgttgcgc
    30721 ttcggtcgac cccaaacgcg ccgctttttc gtaccagtgc agcgcatgat tttcatcttt
    30781 tgcgatatct tttgtaccgt acaaatacat ttcggctaac aaaaatatcg cctctattcg
    30841 cccacgctgc gctgcaccag taagccattg aatagcctca gcgttatttt caggatcatc
    30901 attgttggtt aaataatatt ttcccaattc atacatggca tcccctgccc catctgctcc
    30961 attttttgct gctttggtaa accagtacag cgctttttcc tggtcctgtg gaatactctc
    31021 actttcgcta tacaacctgg cagtatgata ttgcgcatat aaatatccct gttctgctga
    31081 ttgcaaaaac caacgatggg cctgaacctc atcgcgctcc acgccaatcc cttgtatata
    31141 catttgtccc aactcatact gggcaaggtc atgtccttga gtggcggcag ataaatacca
    31201 ctgtaatgct tcttcataat ttcgtaatgg ctcatccgca aagcgtgaga tacgggcaag
    31261 cttatattgc gcagatgcgt tgccattttg ggcagcaagt aaaaaccatt ttcgtgcctc
    31321 actaatattc ttatccgtgc cccttccatc gctatacatg atgcccagcg catactgagc
    31381 atcgataata ttatgctgcg cggcgcgggt aaaccagtta aaggctaatt ttagatcctg
    31441 tgaaacctca tcaccttcgt aatacatttt gccaagagaa taccaggcaa gtgaatagcc
    31501 ttgctctgca gcatgtaagc aggactggaa tacagaagat ggataataaa aaagaccttt
    31561 tagttttatg gcatttagca actttttata tccactatga tgttgcattg ctaaattatt
    31621 gaagcgttct gttaatttcc acttgcgaat cattttttct tttcgccagg aacatgataa
    31681 atactctatt aacaatgcca tatagttgtc tgatagttca aattcagcga ctttatttaa
    31741 tactgcgata tcaaaccatt tcaggctctc ttcattatcc tttaatgatt gataaatcgt
    31801 agccatttcc ttcccgtttt ccatttcttc cagtggagac aagttactgg caaagaaagg
    31861 ggtaatttgt ttttcgatgc gataataaag aagacaactt tcgttaattt ctgtctggat
    31921 tgcgggttga cggctgcctg caactaaatg ccatctgaac gtttgtgctg cagcgagagt
    31981 taataatggc cggaataccc gatgaagatg cagtaataac gcttcttcaa agcacattcg
    32041 tgagctgaga tcttttttct caaggaaaga taacgcctta gcaagttgca tgggggcatg
    32101 ttcgggggtt gtttcattct cctcacagag catatccagc acggcttgcc aggtgaactc
    32161 cccctctgat ttaatagtga catcaggaag tggatgacca gaaaaggtgt tgggtgcgcg
    32221 aaatgtcatc acaggacgag gatgaacaat acccctggac atattgatcg gttgctcttc
    32281 ttcctcttct tgccactgat cttcgtcgtc ataacgtaaa gcctgttcat aagcatcgtg
    32341 caattgttgc caggcttcgg gagaatcttc cgggcggcaa ttttttacca gtttcgcata
    32401 acgacgctta atatctcgtt cttccacgcc tggttgtaaa cctaatattt cccagcagtt
    32461 cattgcagac gttctctttc cagataatct aatgtcggtt caacttcagc acgaaaatca
    32521 gcaagttgat caggatcctg cttctcgagt agataactga aatgacggga gcaattgctt
    32581 atccagtctc gcgtttccgc cagggtgaat tgatagagat tatcaagacg ggcaataaag
    32641 gcctggttta tctgttcatc gcgtggatga atttttaaaa ggcttaattt ggacaacgac
    32701 tgctgtattt gctcttccgt taatgcccct ggattctgtt caataaccag actattttta
    32761 gcccctgtca gtggcacgct gatatcgaca tcaagaatac cgttaatgtc gtacgtaaaa
    32821 cgaacatcaa tacgcacatc accggccggt tttggcggaa cattcactga caactccgca
    32881 aggaaaatat tatcagccac catcctgctt tcaccctggt agattttaaa attaacctct
    32941 gtttgttgat catgtgccgt gtaaaaagca cgaacgcgac tacaaggaat actcctgtta
    33001 cgctcaataa cgggtgcaaa atagcctgat tcgcgggtat gccccagcga tttagaggta
    33061 tcaacgccaa gcgtataagg gcaaacatcg gtcactacta catcttgtaa ttcgctgtgg
    33121 cgcgctttaa gtgcggcctg aatagccgcg ccctgggcaa tgacctcatc cgggtttaaa
    33181 tgcattgccg gtatacgacc aaatagtcgt gtcacaagtt ttcgaataag cggcatacgt
    33241 gtagtgccac ccacaagaac aacctggtct aattggctaa tattaagttt ggcatcgcga
    33301 attgcccgtt ccagtggcat actgattcgc tcgaaaaatg tctgtaataa aaattcaaat
    33361 ttttctgtag tcagatgcca gtaatatcgc cttccttcaa tctccacgct gaaagtagct
    33421 tcgtctcgca tactaagcac gcgcttgaca cgctcagcct caatgcgtaa atggcgttgg
    33481 agtgtcggtt cacgccattc aatattttca ggaagatccc gccttgagca atatgcatcg
    33541 atcaggatat cgacaatatc ttcaccacct aaaaagttat ccccggcact ggctcgtact
    33601 tccataatat tgtcaaacaa ctccagaatg gagacatcga atgtaccacc acctaaatcg
    33661 aataccagaa attgatgttc atcatcttta ttatgcaaac catacgccag tgcagcggcg
    33721 gttggttcgt taattaaacg ttcgacgttc aagcctgcca ttgtcgcagc ggcttttgtt
    33781 gctttacgtt gcatatcacc aaagtaagca ggtacactaa ttactgcttc agttatctct
    33841 tcacctaaat agttttctgc atcttctttt agctgacgta atatcagtga tgaaagttct
    33901 tccgcacgaa actcccgtcc tcccagtttc aacgtctttg atgtgcccat catgcgttta
    33961 aaatttgatg cgcagttttg tggtttgatg agttgcaggt cacgagctgc ctcccctgtc
    34021 aaaaccatac catcttcatc gacacaaact acgctgggtg ttaaaaactt gcctaacgca
    34081 ttggggatca gttcactttg tccatttcgc cagactgctg ccagactgtt agtcgtccct
    34141 agatcgatac cgataatcat aattatcctc ttcaaataaa tgaactgttc cttttataga
    34201 gcagttatat atattc
//
LOCUS       DAJOSY010000041        34018 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJOSY010000041 DAJOSY010000000
VERSION     DAJOSY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.041"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..264
                     /locus_tag="OHN27_002053"
     CDS             <1..264
                     /locus_tag="OHN27_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2286495.1"
                     /translation="LGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAK
                     NLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI"
     gene            complement(616..759)
                     /locus_tag="OHN27_002054"
                     /pseudo
     CDS             complement(616..759)
                     /locus_tag="OHN27_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            825..959
                     /locus_tag="OHN27_002055"
                     /pseudo
     CDS             825..959
                     /locus_tag="OHN27_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
     gene            956..1159
                     /locus_tag="OHN27_002056"
     CDS             956..1159
                     /locus_tag="OHN27_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286496.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLAELINKVAENGDS
                     LRVVDERDSTETPATYASPHQLL"
     gene            1172..1285
                     /locus_tag="OHN27_002057"
     CDS             1172..1285
                     /locus_tag="OHN27_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCQ2286497.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNQ"
     gene            1444..2472
                     /locus_tag="OHN27_002058"
                     /pseudo
     CDS             1444..2472
                     /locus_tag="OHN27_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632466.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2926..3108
                     /locus_tag="OHN27_002059"
     CDS             2926..3108
                     /locus_tag="OHN27_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCQ2286498.1"
                     /translation="MNHPVTGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALQHTEEGCC"
     gene            complement(3209..3538)
                     /locus_tag="OHN27_002060"
     CDS             complement(3209..3538)
                     /locus_tag="OHN27_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCQ2286499.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(3710..4768)
                     /locus_tag="OHN27_002061"
     CDS             complement(3710..4768)
                     /locus_tag="OHN27_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCQ2286500.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFVNTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(4966..5439)
                     /gene="sbmC"
                     /locus_tag="OHN27_002062"
     CDS             complement(4966..5439)
                     /gene="sbmC"
                     /locus_tag="OHN27_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCQ2286501.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVQKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAMAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGDWDIEMYVAVQPKHH"
     gene            complement(5558..6724)
                     /gene="dacD"
                     /locus_tag="OHN27_002063"
     CDS             complement(5558..6724)
                     /gene="dacD"
                     /locus_tag="OHN27_002063"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCQ2286502.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPQIQAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVIMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            6933..8360
                     /gene="sbcB"
                     /locus_tag="OHN27_002064"
     CDS             6933..8360
                     /gene="sbcB"
                     /locus_tag="OHN27_002064"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCQ2286503.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDANRLGINRQHCLDNL
                     KILRENPQVREEVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(8403..8630)
                     /locus_tag="OHN27_002065"
     CDS             complement(8403..8630)
                     /locus_tag="OHN27_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCQ2286504.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(8644..9702)
                     /gene="yeeE"
                     /locus_tag="OHN27_002066"
     CDS             complement(8644..9702)
                     /gene="yeeE"
                     /locus_tag="OHN27_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCQ2286505.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQYYSTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(9882..11240)
                     /gene="plaP"
                     /locus_tag="OHN27_002067"
     CDS             complement(9882..11240)
                     /gene="plaP"
                     /locus_tag="OHN27_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCQ2286506.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(11230..11292)
                     /gene="yoeI"
                     /locus_tag="OHN27_002068"
     CDS             complement(11230..11292)
                     /gene="yoeI"
                     /locus_tag="OHN27_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCQ2286507.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(11507..12436)
                     /locus_tag="OHN27_002069"
     CDS             complement(11507..12436)
                     /locus_tag="OHN27_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2286508.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(12482..13306)
                     /locus_tag="OHN27_002070"
     CDS             complement(12482..13306)
                     /locus_tag="OHN27_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2286509.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(13389..13643)
                     /gene="yoeB"
                     /locus_tag="OHN27_002071"
     CDS             complement(13389..13643)
                     /gene="yoeB"
                     /locus_tag="OHN27_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCQ2286510.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(13640..13891)
                     /gene="yefM"
                     /locus_tag="OHN27_002072"
     CDS             complement(13640..13891)
                     /gene="yefM"
                     /locus_tag="OHN27_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCQ2286511.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            14175..14225
                     /gene="hisL"
                     /locus_tag="OHN27_002073"
     CDS             14175..14225
                     /gene="hisL"
                     /locus_tag="OHN27_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCQ2286512.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    14201..14324
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            14371..15270
                     /gene="hisG"
                     /locus_tag="OHN27_002074"
     CDS             14371..15270
                     /gene="hisG"
                     /locus_tag="OHN27_002074"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCQ2286513.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            15276..16580
                     /gene="hisD"
                     /locus_tag="OHN27_002075"
     CDS             15276..16580
                     /gene="hisD"
                     /locus_tag="OHN27_002075"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCQ2286514.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVDSVGLYIPGGSAPLFSTVLMLATPARIANCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERLLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            16577..17647
                     /gene="hisC"
                     /locus_tag="OHN27_002076"
     CDS             16577..17647
                     /gene="hisC"
                     /locus_tag="OHN27_002076"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCQ2286515.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGIKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKKISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            17647..18714
                     /gene="hisB"
                     /locus_tag="OHN27_002077"
     CDS             17647..18714
                     /gene="hisB"
                     /locus_tag="OHN27_002077"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCQ2286516.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTALALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            18714..19304
                     /gene="hisH"
                     /locus_tag="OHN27_002078"
     CDS             18714..19304
                     /gene="hisH"
                     /locus_tag="OHN27_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCQ2286517.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGANLLKNFLEM"
     gene            19304..20041
                     /gene="hisA"
                     /locus_tag="OHN27_002079"
     CDS             19304..20041
                     /gene="hisA"
                     /locus_tag="OHN27_002079"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCQ2286518.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            20023..20799
                     /gene="hisF"
                     /locus_tag="OHN27_002080"
     CDS             20023..20799
                     /gene="hisF"
                     /locus_tag="OHN27_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCQ2286519.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINISELKAYLATQGVEIRIC"
     gene            20793..21401
                     /locus_tag="OHN27_002081"
     CDS             20793..21401
                     /locus_tag="OHN27_002081"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCQ2286520.1"
                     /translation="MLTEQQRHELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVNDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH"
     gene            complement(21440..22555)
                     /locus_tag="OHN27_002082"
     CDS             complement(21440..22555)
                     /locus_tag="OHN27_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCQ2286521.1"
                     /translation="MRVLHVYKTYYPDTYGGIEQVIYQLSQGCARRGIAADVFTFSPD
                     KETGPVAYEDHRVIYNKQLFEIASTPFSLKALKRFKQIKDDYDIINYHFPFPFMDMLH
                     LSARPDARTVVTYHSDIVKQKRLMKLYQPLQERFLASVDCIVASSPNYVASSQTLKKY
                     QDKTVVIPFGLEQHDVQHDPQRVAHWRETVGDNFFLFVGAFRYYKGLHILLDAAERSR
                     LPVVIVGGGPLEAEVRREAQQRGLSNVVFTGMLNDEDKYILFQLCRGVVFPSHLRSEA
                     FGITLLEGARFARPLISCEIGTGTSFINQDKVSGCVIPPNDSQALVEAMNELWNNEET
                     SNRYGENSRRRFEEMFTADHMIDAYVNLYTTLLESKS"
     gene            complement(22565..23710)
                     /locus_tag="OHN27_002083"
     CDS             complement(22565..23710)
                     /locus_tag="OHN27_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCQ2286522.1"
                     /translation="MKIIFATEPIKYPLTGIGRYSLELVKRLAVAREIEELKLFHGAS
                     FIDQIPQVENKSDSKASNHGRLSAFLRRQPLLIEAYRLLHPRRQAWALRDYKDYIYHG
                     PNFYLPHRLERAVTTFHDISIFTCPEYHPKDRVRYMEKSLHESLDSAKLILTVSDFSR
                     NEIIRLFNYPADRIVTTKLACSSDYIPRSPAECLPVLQKYQLAWQGYALYIGTMEPRK
                     NIRGLLQAYQLLPMETRMRYPLILSGYRGWEDDVLWQLVERGTREGWIRYLGYVPDED
                     LPYLYAAARTFVYPSFYEGFGLPILEAMSCGVPVVCSNVTSLPEVVGDAGLVADPNDV
                     DAISAHILQSLQDDSWREIATARGLAQAKQFSWENCTTQTINAYKLL"
     gene            complement(23757..26279)
                     /gene="wbdA"
                     /locus_tag="OHN27_002084"
     CDS             complement(23757..26279)
                     /gene="wbdA"
                     /locus_tag="OHN27_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105458066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymannosyl
                     GlcNAc-diphospho-ditrans,octacis-undecaprenol
                     2,3-alpha-mannosylpolymerase WbdA"
                     /protein_id="HCQ2286523.1"
                     /translation="MHILIDVQGYQSESKVRGIGRSTLAMSRAIIENAGEHRVSILIN
                     GMYPIDNINDVKMAYRDLLTDEDMFIFSAVAPTAYRHIENHGRSKAAQAARDIAIANI
                     APDIVYVISFFEGHSDSYTVSIPADNVPWKTVCVCHDLIPLLNKERYLGDPNFREFYM
                     NKLAEFERADAIFAISQSAAQEVIEYTDIASDRVLNISSAVGEEFAVIDYSAERIQSL
                     KDKYSLPDEFILSLAMIEPRKNIEALIHAYSLLPAELQQRYPMVLAYKVQPEQLERIL
                     RLAESYGLSRSQLIFTGFLTDDDLIALYNLCKLFVFPSLHEGFGLPPLEAMRCGAATL
                     GSNITSLPEVIGWEDAMFNPHDVQDIRRVMEKALTDEAFYRELKAHALAQSAKFSWAN
                     TAHLAIEGFTRLLQSSQETDAGQAESVTASRLQMMQKIDALSEVDRLGLAWAVARNSF
                     KRHTRKLLVDISVLAQHDAKTGIQRVSRSILSELLKSGVPGYEVSAVYYTPGECYRYA
                     NQYLSSHFPGEFGADEPVLFSKDDVLIATDLTAHLFPELVTQIDSMRAAGAFACFVVH
                     DILPLRRPEWSIEGIQRDFPIWLSCLAEHADRLICVSASVAEDVKAWIAENRHWVKPN
                     PLQTVSNFHLGADLDASVPSTGMPDNAQALLAAMAAAPSFIMVGTMEPRKGHAQTLAA
                     FEELWREGKDYNLFIVGKQGWNVDSLCEKLRHHPQLNKKLFWLQNISDEFLAELYARS
                     RALIFASQGEGFGLPLIEAAQKKLPVIIRDIPVFKEIAQEHAWYFSGEAPSDIAKAVE
                     EWLALYEQNAHPRSENINWLTWKQSAEFLLKNLPIIAPAAKQ"
     gene            complement(26290..28416)
                     /locus_tag="OHN27_002085"
     CDS             complement(26290..28416)
                     /locus_tag="OHN27_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012967601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCQ2286524.1"
                     /translation="MTKDLNTLVSELPEIYQTIFGHPEWDGDAARDCNQRLDLITEQY
                     DNLSRALGRPLNVLDLGCAQGFFSLSLASKGATIVGIDFQQENINVCRALAEENPDFA
                     AEFRVGRIEEVIAALEEGEFDLAIGLSVFHHIVHLHGIDEVKRLLSRLADVTQAVILE
                     LAVKEEPLYWGVSQPDDPRELIEQCAFYRLIGEFDTHLSPVPRPMYLVSNHRVLINDF
                     NQPFQHWQNQPYAGAGLAHKRSRRYFFGEDYVCKFFYYDMPHGILTAEESQRNKNELH
                     NEMCFLAQPPTGFDTPALLAHGENAQSGWLVMEKLPGSLLSDMLVAGEEIDREKIIGT
                     LLRSLAVLEKQGLWHDDVRPWNVMVDARQHARLIDFGSIVTTPQDCSWPANLVQSFFV
                     FVNELFAENKSWNGFWRSAPVHPFNLPQPWSNWLYAVWQEPVERWNFALLLALFEKKA
                     KLPSAEQQRGATEQWIIAQETVLLELQSRVLNESAGSEALRGQIHTLEQQMAQLQSAQ
                     DAFVEKAQQPVEVSHELTWLGENMEQLAALLQTAQAHAQADVQPELPPETAELLQRLE
                     AANREIHHLSNENQQLRQEIEKIHRSRSWRMTKGYRYLGLQIHLLRQYGFVQRCKHFI
                     KRVLRFVFSFMRKHPQVKHTTVNGLHKLGLYQPAYRLYRRMNPLPHSQYQADAQILSQ
                     TELQVMHPELLPPVVYEIYLKLTKNK"
     gene            complement(28436..29731)
                     /locus_tag="OHN27_002086"
     CDS             complement(28436..29731)
                     /locus_tag="OHN27_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family O-antigen ABC transporter ATP-binding
                     protein Wzt"
                     /protein_id="HCQ2286525.1"
                     /translation="MSIKVQHVGKAYKYYPSKWNRVIEKLLPGDKPRHSKKWVLKDIN
                     FSIEPGEAVGIVGVNGAGKSTLLKLLTGTTQPTKGSIEIQGRVAALLELGMGFHPDFT
                     GRQNVYMSGLMMGLGREEIERLMPEIEAFADIGDYIEEPVRIYSSGMQMRLAFAVATA
                     SRPDILIVDEALSVGDSRFQAKCYARIADFKEQGTTLLLVSHSAGDIVKHCDRAIFLK
                     NGDICMDGTARDVTNRYLDELFGKPDKDSATKSATAISSASGESQMSLDEIEDVYHTR
                     PGYRPEEYRWGQGGAKIIDYHIQSAGVDFPPSLTGNQQTDFLMKVVFEYDFDCVVPGI
                     LIKTLDGLFLYGTNSFLASEGRENISVSRGDVRVFKFSLPVDLNSGDYLLSFGISAGN
                     PQTDMTPLDRRYDSIILHVTKSMDFWGVIDLKSSFTSYQ"
     gene            complement(29734..30519)
                     /locus_tag="OHN27_002087"
     CDS             complement(29734..30519)
                     /locus_tag="OHN27_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family O-antigen ABC transporter permease
                     subunit Wzm"
                     /protein_id="HCQ2286526.1"
                     /translation="MFSAIYRYRGFIIDSVKRDFQSRYQTSFLGAAWLILQPIAMISV
                     YTLIFSELMRARLAGMDGPFAYSIYLCSGVLTWGLFTEMLNSLVNVFLTNANILKKLS
                     FPRICLPIIVTASAFINFLIIFGLFVLFLIVTGNFPGMIFFEIIPVLIVQMLFTLGLG
                     IILGVLNVFVRDVGQFVNILLQFWFWFTPIVYVSKTLPEWVSGLLAYNPMATIIGSYQ
                     NVMLYHQSPNWMALLPVTVVSVILFLFAWRLFKKHAADIVDEI"
     gene            complement(30525..31907)
                     /gene="rfbK2"
                     /locus_tag="OHN27_002088"
     CDS             complement(30525..31907)
                     /gene="rfbK2"
                     /locus_tag="OHN27_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_166649022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family phosphomannomutase RfbK2"
                     /protein_id="HCQ2286527.1"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVG
                     GDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     NYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLRQISVLKEYVDHLM
                     GYVDLANFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGI
                     PNPLLPECRQDTADAVREHQADMGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFL
                     QKQPGAKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHH
                     YFRDFAYCDSGMIPWLLVAELLCLKNSSLKSLVADRQAAFPASGEINRKLGNAAEAIA
                     RIRAQYEPAAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADVALMNEKTTE
                     LLNLLKEELL"
     gene            complement(31931..33346)
                     /locus_tag="OHN27_002089"
     CDS             complement(31931..33346)
                     /locus_tag="OHN27_002089"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HCQ2286528.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRL
                     SGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRYG
                     DDPLMLVLAADHIINNQSAFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVA
                     LTDSAHAPYQVARFVEKPDRERAEVYLASGEYYWNSGMFMFRAKKYLSELAKYRPDIL
                     ETCQAAVNAADNGSDFINIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSW
                     SALWEVSPKDGQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMN
                     RKRSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFS
                     MQMHHHRAEHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEI
                     QSGSYLGEDDIIRIKDQYGRC"
     gene            complement(33626..33910)
                     /locus_tag="OHN27_002090"
     CDS             complement(33626..33910)
                     /locus_tag="OHN27_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286529.1"
                     /translation="MSRLSRLSERSAVAMCKGQPGPQARCLLSGEDDVSSHLSLYREC
                     PSMTVTGIGKPPVSFSSVAVRACVLPCGNEKVLMVNPAANAESVVRLAAR"
BASE COUNT         8498 a         9125 c         8642 g         7753 t
ORIGIN      
        1 ttgggacttg aaccgaagaa cacggcggtg cggagtccgg agagtaacgg aatagcagag
       61 agcttcgtga aaacgataaa gcgtgactac atcagtatca tgcccaaacc agacgggtta
      121 acggcagcaa agaaccttgc agaggcgttc gagcattata acgaatggca tccgcatagt
      181 gcgctgggtt atcgctcgcc acgggaatat ctgcggcagc gggcttgtaa tgggttaagt
      241 gataacagat gtctggaaat ataggggcaa atccacggtt catgtatccc ctgagctgac
      301 agtgtaccgt gtatattaag cgtaaccgac aggttctcct tcataccata ttaaattttc
      361 caggtattgc cgctgcctta tttcgtccca tacaggaaaa ctcatctggg tttctgacaa
      421 atacgcgaaa ctcacagccc ctcaatatca accacacgtt gtaagccggt aatacctgaa
      481 aatcagccta ttaccgccca cgcctccggc accattccct tttgatttcg aattctttgt
      541 tgtcgctata ccgcttaatg ttcccggtcc tccggtgcag ataacaccgg agtcatcgta
      601 gcgctgcttc aatattcagg tgacatattt ccagacggaa gccggcgcgc gatcgtaaag
      661 tttgttcatt gtcagttctg ccagccggtt gtcaacggca gagttaaagg cttccagctc
      721 cgcaagggta agtttataac gcgtgtggga attcactttt cccgcgtctc ctgacgtatt
      781 acgggctgcc acttacctgg tgtcccgcaa tacagcctta gtgttccgtt acccactctc
      841 agttaataaa cagcatcgat atcctccggg ctcgccgggc aacaggcctg atgacccgcg
      901 acaattacag aacggtaaat aacattaccc tggggaagca tccggaggcg aaacgatgaa
      961 actggccctg acgctggaag ccgacagcgt taacgtgcag gcgctgaaca tggggcgcat
     1021 tgtcgttgac gtcgatggtg ttaatctcgc tgaactgatt aacaaggtcg ctgaaaacgg
     1081 tgattcactc cgcgtggtgg atgaacgtga ctctaccgaa acaccggcaa cttatgccag
     1141 ccctcaccag ttgctgtaag acaatgcagt gatacagggt atagtgccat taaccacaga
     1201 aacagcactg ttgtcctgta ttaagcaggc aagagaagat gtccttaccc tgcgccatct
     1261 gcagcttctg caccagaacc agtgaatctc actcaacagg cttcatttcc cctgacgcca
     1321 gcctgaatac agctggcgtt ttcatttata aacagaaagg aaaaccgata ataatggaat
     1381 ggacgcccct accatgaagc aacggtagga tattactgat aaaccttcca gtggaggtgc
     1441 gttatggccg ttttaacgat tagcattgac ctgacaaaaa atgaattcca aatccacggt
     1501 ctgggtagga acagaaaaat ctaagctcag aaaacggatt aaacgttccc tgatgcccgt
     1561 attcttcgcg aatatgtctt cctgtcggat tgggatggag gtctgcgccg gagcgcgtca
     1621 ttagtgagaa tgctgtctac ttaggggcat aatgtcagac taatgcccgc gatttgtaaa
     1681 gccatatgtg aaaacaaaca agaatgatat ggctgctgcc gaggccatct gtgaagccgt
     1741 tacccgacct aatatgcggt ttgtcagcat caagacgcct gagcagtagt cattactgtc
     1801 ttttcatcgt gcccagacgg ggtttgttaa atcaaggact gcgcagataa accagatcag
     1861 aggattactg gctgaatttg gcatcgttct cccgcccgac actgtagcga tcagtcgtca
     1921 tgtccctgcc ctgcttgaag acgcggaaaa gtgtttaaca atgccgttca gacagctgtc
     1981 gtcctatctc taatgaaaat gtgaaacaac tgagcgtaca cattgaaacc atagaagccg
     2041 tgagcaacga acacgtcggg aacgacgaac tgtgcaggaa aattgccatc atttcaggga
     2101 ttgctattct taccaccact gcaattgtca gcaccgggta gtatcggctt caaaaaccgg
     2161 cgtcagcttg ctgcatggct tggactggtt ccccgacagt gcttcagcgt aggtaaaaac
     2221 acgctggggt ctatcagtaa acgggacgat gtctatatca gaacgctact gttccatggt
     2281 gcgaagacag tgtcaaacgc cagaaagggc agacaggtcc aggaaagctg gctgggcaga
     2341 ccagtctcgc ggcggaataa aaatgttgcc accgtagcgc tgaacaacaa aaatgccaga
     2401 gtgctctggg tattgctaac caacgataaa gagatttcac cagaagaagt gatggtagct
     2461 gcatatatgt aaatgaaact acttctgtct gttgaaaaat aaaaccaacc tactgattgc
     2521 taaggataaa cgagtattaa aggccagact gataagaccg cgactaagtg aacctgtatc
     2581 tgacatggca tagcttcgct aatgtgtcat catgattagg actaaatctg tgaattcctt
     2641 taggaagcac ggacacaggc caatctgagt ccatatgtat gattggaatc ttacacagat
     2701 gactttttga cacggggact tggcaaacag gagcgaccat gtatgtcaga tattacaatc
     2761 tcacttccga aagaagtcaa cgggcacgtt atctgttcaa ccagaatccg acacattctg
     2821 gctatacgaa agagcatact tatgcaaatc tatatgcttt tccggcaaca agcaggaatc
     2881 tcagcgctga ggcaggaatt agaggtaaaa acgccctacc gggccatgaa tcacccggtc
     2941 actggagtag taaccaaagc agatttagcc agcatggaac acatatcttt ggtgaaatgt
     3001 tggttacggg aagctggagc gcacaacgtg ttagtaacca gtgcggttaa taacaatgga
     3061 gttacagaac tttttgcctt gcagcataca gaagaaggct gttgctaatt tcgataggtt
     3121 ctcagatgag aatgatgacg gtgatacaca gataagaatc aatgagcaat gagggttgcc
     3181 gggcaaccct cattgaataa aacaggaatt acttcgcttc gccgtttttc atttcgccca
     3241 tagctttcag ctttttggag atatcgcggc gttctttgga gagctcggca tttttgatga
     3301 tgtaatcgtc aacgcgatct tcatagtcac ctttcatgct ggcgatgatg ccctggattg
     3361 cttcaacgct catccctggc ttgatgtaat cgctcaggtt gtccagcaac aggacgcgct
     3421 tctggttgtc acggatcttt ttctcaacgt cctgaatttc acgttgcagt ttgttcttac
     3481 gacggaacag acgaacaaat tccagtacgt cctggaatga aggcttggta gtttccattt
     3541 ttatacccct gataatgtga gagtcggatt cgtttaagca accgctattc gttagggcca
     3601 acattactca tagctgccgc agatgacaat gcttttatcc cttttcacta tcataccctt
     3661 tatccttgct gaatcgaagc agcagcaaga tgattctgaa gttcaggaat tatttgcgca
     3721 aggcccggca aatcagattc gatagcaact caagctgatg tgccgtttcc atgttcagcc
     3781 acacataacc atagattggt gtttccacga ctttcaggtc atggtggtta tcgagcaact
     3841 gacgcaactc ttccacagca tcgttcaatt tttccgtatt gacaaaaacc ggctgcggat
     3901 taccttcgta cagcgcatga acaaggctca gcagtatttg ctgcatcatg tgctgggtat
     3961 cacgaagttt ttgcgcattc aataacacga aatggctggg gcgcgtggcc cagtatgcat
     4021 tgatttgcaa ctccagcata caaaccagat tgcgattaat ggtctggata ccttcatata
     4081 tcgatttggg aatacgggtt tctttgctgg cgggcgcaat cagcccacgc attttcacgg
     4141 catcggtcag cagtttttgt agatggcttt ccagacgtgg gcgttcgagt aagttcggtg
     4201 agaatgcaga ttgatagacc cgattatact cggtcagact tttcgccagt tgaatgcgcc
     4261 agtggatgaa cgcccgttgt ggccagatac cggtaaacaa cattgccagt aaagagccga
     4321 ggatcacatc gccgcttcgc cataacgccg tatcaatttc acctgtcggg gaacccacaa
     4381 caattgccag cgtcacccca atcaataatc cttgatacgg tttcttgccc agcgccagcc
     4441 aaccgcaaag aaacatcgcc gccgcgcacc agactaacat cagcggtaac gaaattaact
     4501 ccagttgcag agcgataaga cctaaaatcg accccaacac cgtaccgcca atacgttcga
     4561 aggcgcgagg gacaacgttc ccccagaacg agattggccc cataatcacc accatggtga
     4621 ccagcggcca ggtgctttcc gggatagtaa acaggcggat aatgagaaaa gtgagcagga
     4681 atgccagcgc gacccgagta ccatgcacaa tgcggtaatg gcggtatacc cggatttcaa
     4741 acgggcttaa tgacttatcg gcacgcacgc agaaactccg taatgaagat taaaagcgct
     4801 cataacacat ctgtcggcca gaaggcgcgc ctctgagtta tccgacgaac ataccgggaa
     4861 cagtgtaatt tcccggcagg agcgaaaaat ttgaaaggcg catcttactc ttttcgcttt
     4921 caaaaaaaga gtggtcttcg cgttaacaca ccgccctgag atgaattagt gatgttttgg
     4981 ctgcaccgca acatacattt cgatatccca gtccccatct tccgcgccat tgttcaagta
     5041 aacctcgaag cagggctttg gtaacatttc ataagcactg tcctgcaaga ggctattaaa
     5101 gaactgatac cagggtttag caaaatcatc accgactaca cgagccaccg ccatcgcata
     5161 ctgaccacct gaaatttctg tcagaatgac gccctcactg ttttcgggga gcgtaaagtt
     5221 attcggtacc gtcacgacgg tgtcgcagcg taatttttcg gcgggtgttt catctggatt
     5281 gtcgtaatag acagcaaccc actccttcgg cacaatattt ttgctatcta cccacatcat
     5341 caactgctca aaaccttttt gtaccgtctg ctcccacggg ccaacgagat ggaaacctgc
     5401 aacggtacgt ttatcttcct gcttaatctc gtagttcatg acgcctccat taacactgtt
     5461 tttatataca gtataattgc aatattaaaa ccacaaggaa tgagtgttga ttatgcgagc
     5521 agactcgcac tcctgccagt ctgctgcaaa agaaaggtca ggccttatgg tggaaataat
     5581 cactcaggcg ggaaaacatg ctgccttccc cgacagattc cagggtaacc agcggccagt
     5641 gcgccacttg tttatcacgg tcgtaaagtt caatttcccc tacccgctga tgggcgctaa
     5701 ttggcgcggt gagttcttta ccatcaaggg tatatttggc tttgatatgt ggaatttcgg
     5761 ctttcggtag caccatccag aactcttgtt ccgttcccag ggcgatattt tctttatcgc
     5821 cataccagat gcgttccgta ccaacctttt tcccacggtg caaaatttgc accgtagtaa
     5881 agttttgttg accccaacgc agtaattttc ttgcctcttc ctcacgacct tttgcgctgt
     5941 cagcacccat tatcactgca atgagacgac gctgcccatc tacagccgaa gcaatgaggt
     6001 taaaaccggc accagaagta tggcccgttt tcaggccgtc aacattcatg gttttatccc
     6061 acaataaccc gttacggttt tgctgggtga taccgttcca ggtgaggctt ttctcactgt
     6121 acatatgata aaactcgggc tcgccgtgga tgatagcgcg agaaagcacg gctaaatcat
     6181 aagccgagct atgctggcct ggtgcatcca gaccatgcac tgtttcaaaa tgcgtatcct
     6241 tgagatgcag cttctcggca tagttgttca tcatttcaac aaactgccgt tgcccaccgg
     6301 caatatagtc agccagagca acacaagcgt catttccgga atccacaatt aaaccacggc
     6361 ttaaatcacg taccgatacg cgatcgccct ctttcaaaaa catcagtgaa gaaccgacaa
     6421 acaccggatt atctttcgcc cacgcatcac gccccacggt gacaatatcg tctggcgtaa
     6481 tgcgatgact atcgatagcg cgatccacga cataacccgt catcagcttt gtcaggctgg
     6541 cgggattgcg ctgttgatgc tcattacccg cggtgagaat ctgaccggtg gtgtaatcca
     6601 tcagtaccca ggacccggct tgaatctgtg gaggctgagg tgaaaaagga atgttttccg
     6661 ccgcaaaacc agacgataag ttaaaaacga acaaagaagc agcaataata agacggcgtt
     6721 tcaacagcaa accctcagga gtttcaaata gctgttcttt ttacggaaat acttatgaac
     6781 tggctggaat aaagtgcaag aaaatgtgac taccctccca tttttatctg acatgatctg
     6841 ttgccactcg ctgtcaaatt atcgcgctaa agctgattag cacggtgata tttgatactc
     6901 tggcagacag cagaaataac ggatttaacc taatgatgaa tgacggtaag caacaatcta
     6961 cctttttgtt tcacgattac gaaacctttg gcacgcaccc cgcgttagat cgccctgcac
     7021 agttcgcagc cattcgcacc gatagcgaat tcaatgtcat cggcgaaccc gaagtctttt
     7081 actgcaagcc cgcggatgac tatttacccc agcctggagc agtattaatt accggtatta
     7141 ccccgcagga agctcgggcg aaaggagaaa acgaagccgc atttgccgcc cgtattcact
     7201 cgctttttac cgtaccgaag acctgtattc tgggctacaa caatgtgcgt ttcgacgatg
     7261 aagtcacacg caacgttttt tatcgtaatt tctacgatcc ttacgcctgg agctggcagc
     7321 atgataactc gcgctgggat ttactggatg ttatgcgtgc ctgttatgcc ctgcgcccgg
     7381 aaggaataaa ctggcctgaa aatgatgacg gtctaccgag ctttcgcctt gagcatttaa
     7441 ccaaagcgaa tggtattgaa catagcaacg cccacgatgc gatggctgat gtgtacgcca
     7501 ctattgcgat ggcaaagctg gtaaaaacgc gtcagccacg cctgtttgat tatctcttta
     7561 cccatcgcaa taaacacaaa ctgatggcgt tgattgatgt tccgcagatg aaacccctgg
     7621 ttcacgtttc cggaatgttt ggggcatggc gcggcaatac cagctgggta gcaccgctgg
     7681 cgtggcatcc tgaaaatcgc aatgccgtaa ttatggtgga tttggcagga gacatttcgc
     7741 cattactgga actggatagc gacacattgc gcgagcgtct atataccgca aaagccgatc
     7801 ttggcgataa cgccgccgtt ccggttaaac tggtgcatat caataaatgt ccggtgctgg
     7861 cccaggcgaa tacgctacgc ccggaagatg ccaaccgact gggaattaat cgtcagcatt
     7921 gcctcgataa cctgaaaatt ctgcgtgaaa atccgcaagt gcgtgaagaa gtggtggcga
     7981 tattcgcgga agccgaaccg tttacgcctt cagataacgt ggatgcacag ctttataatg
     8041 gctttttcag tgacgccgat cgtgcagcaa tgaaaattgt gcttgaaacc gagccgcgta
     8101 atttaccggc actggatatc acttttgttg ataaacggat tgaaaagctg ttgttcaatt
     8161 atcgggcacg caacttcccg gggacgctgg attatgccga gcagcaacgc tggctggagc
     8221 accgccgcca ggtcttcacg ccagagtttt tacaaggcta tgcagaagag attcagatgc
     8281 tggcgcagca gtatgccgac gataaagaaa aagtggcgct gttaaaagcg ctttggcagt
     8341 acgcggaaga gattgtctaa tgtgaagcct ccgccgctgg tacggcggag gacaatgttg
     8401 agtcaggctt tttgaacggt gatgctccac gctgcatcgc caatctgctg ataatcggtg
     8461 atggcatgtc cttcttctgc cgcccactgt gggattgctt cagttgcctg ggtgcaatca
     8521 aactcaatta ccaactcatc gccgctggcc atttctgcca gtgctgcttt tgcttctatg
     8581 agtggaaacg gacagacctg cgtcaccaca tccagctttt taatcgccat ctttacacct
     8641 tacttaattt gccgcagcag ttgccagtcg cgccttacgc tgcggtcgaa catacacaag
     8701 ccaggacgca gtccagactc cgagaatcat aaatacaagg ccaatccagc cctgccaggt
     8761 catcattgca gtcataacca gtccattacc gatagagcaa ccacctgcaa tactggcacc
     8821 gaagcccatc agtacaccac ctaacccgct acgtaatgtt gtttgtgcat cagctgcgcg
     8881 aacgcggaac tcacggctcg ctttggctgc aataaatgac cccacgaaga tccctaacac
     8941 cagaaaaacg ccccagttaa tgtatttcac gtcacccgcg accagaaatt gcagaatatt
     9001 ggccgttggg gaagtgattc ctaacccaaa catgcgtccg gttgcttcac tcaggggcca
     9061 ggctaaaagc gcaatcaaac cgatgagtac agcagtgaca aagggatgcc agcgtttttc
     9121 aaacagaata tgagcgatcc cggttcggcg cggcggtaag gtcgcgactt tgagttttgg
     9181 cttcttcaac tctttcatca ccacccagag cgttatcacc agcaaaacgg taaccaacgg
     9241 ccacacagac aaattaaaag tctcagcaat agagttatgt tcagtactgt agtactgcaa
     9301 ggtttgattt aaaccactgg cgtgtggaga acgcatcacc gcactcatca ccatataagt
     9361 gaaaagcgcg atccaactgc cgatcaatcc ctcacccgcg cgataccagg tcccggtggc
     9421 acatccaccc gccagaacaa ttcccagccc gaaaagatac ccacctataa cagtaccaag
     9481 ccacgggaac gccccggctt cgtaagtcag taggcccgcc tgaattaacg caaaaacccc
     9541 cacgctttgt accgaaattg caatcagcaa ggcgtaaaac atgcgattat ttttcgcgat
     9601 atacatatcg cgaaaaccac ctgtcaggca aaaacgccca cgctgcatga caaatcccag
     9661 cagagcacca caaattagcc cgcttaatat cattgaaaac ataaattaat taaccatatg
     9721 aatgttaatg gggaaattat tcatgactgg tggaatgcag accaataacc aaattctcta
     9781 aattagaaca aatggttatt agatgaggat ttcgataaaa aagaataaaa aaaccggagc
     9841 caaatgttca ttcgactccg gtttattatt agaaaagatg attatgctac gtcttcgtac
     9901 tgcggaaccg gattacggaa actcttggtt acgcaagcca ggtaaatcag accgatagca
     9961 gcccagatca gacccagaac cattgagctt tcttccaggt taacccacag cgcaccaacg
    10021 gtcagcgcac cacacatcgg caggaacaga tactggaagt gatctttcag cgtcttatta
    10081 cgcttctcac ggatccagaa ctgcgagatg accgacaggt taacgaaggt gaacgccacc
    10141 aacgcaccaa agttaatcag cgccgtagcc attaccaggt cgaagttgat tgccagcaag
    10201 gcaatcgcgc caaccaggat gatgttcatc gccggagtac gccatttcgg gtgtacataa
    10261 ccgaagaagc ttttcgggaa cacgccgtca cgacccatta cgtacatcag acgcgccacg
    10321 cctgcgtgcg ccgccatacc ggacgccagt acggtaatgg tggagaagat cagcgcgcca
    10381 acctggaacg ctttacccgc aacgtacagc atgatttcag gctgtgacgc atccggatct
    10441 ttaaagcgag agatatccgg gaagtacagc tgcaggaagt aagttgcaaa gatgaagatc
    10501 atgccgccga tcagcgcggt caggaaaatc gcacgcggga tcacgcgctc tgcatctttg
    10561 gtttcttccg acaggttgct gatgccgtca aaaccggtaa aggagaagca caggattgtc
    10621 gccccggtaa tcatcgggat aacatgcgca tcaccagacc agaacggacg agtgctcgcc
    10681 agcgtaccag cgccttcacc ttcaaataca ccataaacaa ccatgcccag aatcaccgcg
    10741 atcagcacta cctgcaacac gacgattaca gtgttgaagt tcgctacgga tttcagacta
    10801 cgcaggttaa aggcggtcat aaaggccacc agcgccacca caaacatcca cgatggaatg
    10861 gaaggcacca gagcttcaaa atagattttc gccaacagaa tgttgatcat cggcgcgaac
    10921 agatagtcga gcagagaaga ccaacccacc ataaagccga cagtcgggct aatggatttc
    10981 tgggcgtaag tgtatgcaga gccagcagaa ggatagcggc gaaccagctt cccgtagctc
    11041 agagccgtaa acaggatcgc aatcaatgcg aacgcatagg ctgtcggcac atgaccatcc
    11101 gtgaggcctg aaacgatacc aaatgtatca aacagcgtca tcggctgcat ataggctaga
    11161 cccatcatta caaccggaac taacgtaagc gttttacgca attccacgcg agaggtgttt
    11221 ggagtaacgt tatgcgacat ggtcattctc ctttacggtg ataaccgtcg cgtaagcaaa
    11281 aaattgcccc atttttttgg attcctcagc gacaacaact gtcgattttt agtaaatatc
    11341 tatccggtac gaagcccggc ctcttggtat gaatgattgg tttgaagcaa aaaataaccg
    11401 acgctaatta aacgtcggtt tttagtcatt ttttgacagc ggcgcattgt gcctaaatgg
    11461 gggggaaatg acaagagaat gagaggcttg tcataataat ttttctttaa atggctgatt
    11521 ttccgtcatc agattttgca taaacaccgg cgatcgcact atttgctaaa atttcatctc
    11581 gccaccagcc gctggcaagc cctgcggtct gttcattcca gccaatccag acgggttcaa
    11641 agagagtttg ggcgaccact ttcttctcga ttaacgcgcc actatcgaga aaacgttgcg
    11701 ccagataccg tggtaaatag ccgcagccaa ggccgctaat ttgcagctcc agtttggttt
    11761 taaaatcgaa aacggtaatg gcttcttgtt cgtcgagaag ttgtgaggcc gtagaagcgc
    11821 ctgcctgcgc agtgtccccc accacaatcg cccgatatcg tttaatgata cgacggttta
    11881 acggttcttc ttcctgggct aacggatgat gaggcgcgac cgcaaagact tgttcaagat
    11941 cgcccaaccg cgagaaacca aactcactgg aagatggtgg ctcatgcatc gcgccaacga
    12001 taatatccgc tcgcccctga gtcagggcat cccaggaacc accgagtaca ccattgataa
    12061 acttcaggcg cgtcacgcta tgatgttgat aaaaagcttc aatcagtggc gcaagcagag
    12121 aaaaaggaaa cgtgtcgtcc acgccaatca ccagctcgtt ttcccagcct tcatgcagtt
    12181 tgatcgcctg cttttccagt tctcgcacgg tatgcagaac ttcccgcccc ttctctaata
    12241 acatttttcc ggtacgggtg aatttagcgc ggtgaccgct acgatcaagc aattgaatat
    12301 taaggtcgct ttccagcttg tgaacggtgt aactcagagc ggacggcgtc ttgtagagtt
    12361 tggccgacgc cgccgcaaaa ctgccttctt tttctaaggc atcgaggatc atcaacacat
    12421 ccagcagtgg tttcatacac gcccccttgc agtgcatgcg gcgtcctgct ggcgtgatcg
    12481 tttactccag cggcattacc agcggatcgg gatactggta ttcaaatccc agttcattac
    12541 aaatccgact gccatcaata atcttacctt tgccgctgtc cagactattt ctgaactgcg
    12601 gaggttccag ccccagtaaa cgggccatct gcggatagaa aacattacgc gcagggtgag
    12661 ctggcgcaca tatattatag atgtgtccgc ctttaggtgc ctgtaacaac agagtgatag
    12721 cgccaataac atcttctaaa tggactaaat taacaccatg ttcaccatca ggcgcggttt
    12781 ttccggcaaa gaagcgtccg ggatgacgtc ccggtccgac cagtcccgca agacgcagga
    12841 tatcgaccga agtaccaggt aaattgtgca gccagtcttc gagttcttct aatactcgtc
    12901 cactgttggt taccggatta cgcggggtgg tttctttcac cgtgccttgc gcgtcgccat
    12961 agacagatgt tgagctggta aaaataatgc ggggaatacg atgggccagc gcgctatcca
    13021 ctaactcttg taccgcttgt aaatagaact catcgccggg gccgctacga cgtgccggaa
    13081 gcgtaatgac cagcgcatcg gcatccatca gggcatccag atcgtcagaa tcgcaaacta
    13141 actcaggctc catgcgaagc agatagctat caatgccact cattcgggct gcttcgacac
    13201 catcttgtgt ggttttactc ccggtgactt gccagcctcg cgctgaaagt gacatcgcca
    13261 gcggcatgcc taaccaccct aacccgacaa ttgcgacctt tttcatccgt tatctcctga
    13321 tacaccttag atctataagg ctacgctagc gtatcaaaac tgacaattca ttctatgaat
    13381 gaatctgttc aataatgata acgacatgct gcaatgagca gtgaatcgtc ggtaaccgcg
    13441 tataccagac ggtgctcctc tgtaatgcgt cgggaccaga aacctgacaa attatgtttc
    13501 aggggttctg gcttcccctt accttcaaat ggcgttctgc gggtatcttt gataagttca
    13561 ttgatctttt taacaattcg cttatctgtt tcctgccagt acagataatc atcccatgat
    13621 tcctcagacc agattagttt cactcaataa tgtccttttc cgttcctttg cctgatttca
    13681 ggctatcgat tgagtccatc aatctccggg cgttagcggg ggaacgcagt agataagccg
    13741 tctcttccag cgagttgtat tcttcgagtg acatcagaac acaagcctct ccattctgac
    13801 gagtaatgag gatcggggca tgatcttcaa cggctttcat cattgttgcc gacaaattct
    13861 gacgcgcttc gctgtaacta attgtacgca tgtcaatctc ctcttttgta cagttcattg
    13921 tacaatgata gacgttaatt aactatttat taattagttt gtagatcaag acattgtcag
    13981 tgagacgaaa aatccaggct tcgctatttt tggtaccgtc agcttagagg tcagtcctct
    14041 tagccccctc ctttccccgc tcattcatta aacaaatcca tcgccataaa atatataaaa
    14101 aagcccttgc tttctaacgt gaaagtagtt taggttaaaa gacatcagtt gaataaacat
    14161 tcacagagac ttttatgaca cgcgttcaat ttaaacacca ccatcatcac catcatcctg
    14221 actagtcttt caggcgatgt gtgctggaag acattcagat cttccagtgg tgcatgaacg
    14281 catgagaaag cccccggaag atcaccttcc gggggctttt ttattgcgcg gttgataacg
    14341 gttcagacag atttaaagag gaataacaaa atgacagaca acactcgttt acgcatagct
    14401 atgcagaaat ccggccgttt aagtgatgac tcacgtgaat tgctggcgcg ctgtggcatt
    14461 aaaatcaatc ttcacaccca gcgcctgatc gcgatggcag aaaacatgcc gattgatatt
    14521 ctgcgcgtgc gtgacgacga cattcccggt ctggtgatgg acggcgtggt agaccttggg
    14581 attatcggcg aaaacgtgct ggaagaagaa ctgcttaacc gccgcgccca gggtgaggat
    14641 ccacgctact ttaccctgcg tcgtctggat ttcggcggct gtcgcctttc gctggcaacg
    14701 ccggttgatg aagcctggga cggtccgctc tccttaaacg gtaaacgtat cgccacctct
    14761 tatcctcacc tgctcaagcg ttatctcgac cagaaaggta tctcttttaa atcctgctta
    14821 ctgaacggtt ctgttgaagt tgcgccgcgt gccggactgg cggatgcgat ttgcgatttg
    14881 gtttccaccg gtgccacgct ggaagctaac ggcctgcgcg aagtcgaagt tatctaccgc
    14941 tcgaaagcct gccttatcca gcgcgatggt gaaatggaag aatccaaaca gcagctgatc
    15001 gacaaactgc ttacccgtat tcagggtgtg atccaggcgc gcgaatcaaa atacatcatg
    15061 atgcacgcac cgaccgaacg cctggatgaa gtcatcgccc tgctgccagg tgccgaacgc
    15121 ccaactattc taccgctggc gggtgatcaa caacgcgtag cgatgcacat ggtcagtagc
    15181 gaaaccctgt tctgggaaac gatggaaaaa ctgaaagcgc tgggtgccag ttcaatcctg
    15241 gtcctgccga ttgagaagat gatggagtga tcgccatgag ctttaacaca atcattgact
    15301 ggaatagctg tactgcggag caacaacgcc agctgttaat gcgcccggcg atctccgctt
    15361 ctgaaagcat tacccgcact gttaacgata ttctcgataa cgtgaaagca cgcggcgatg
    15421 aggccctgcg ggaatacagc gcgaagtttg ataaaaccac ggttaccgcg ctgaaggtgt
    15481 ctgctgagga aattgccgcc gccagcgaac gcctgagcga cgagctaaaa caggcgatgg
    15541 cggtggcagt aaagaatatt gaaaccttcc acactgcgca aaaactgccg ccggtagatg
    15601 tagaaacgca gccaggcgta cgttgccagc aagtcacgcg cccggtagat tcagtgggtt
    15661 tgtatattcc tggcggctcc gccccgctct tctcaacggt attaatgctg gcaactccgg
    15721 cgcgtattgc gaactgtaaa aaagtggtgc tgtgctcacc gccgccgatt gccgatgaga
    15781 tcctttacgc ggcgcagctg tgcggtgtac aggacgtgtt taacgtcggc ggcgcacagg
    15841 ccattgccgc gctggcgttt ggtacggaat ctgtgccgaa agtggacaaa atcttcgggc
    15901 cgggtaacgc ctttgtcacc gaggcaaaac gtcaggtgag ccagcgtctg gacggtgcgg
    15961 cgatcgatat gcccgcaggc ccttcggaag tgctggtgat tgctgacagc ggcgcaacgc
    16021 cggatttcgt ggcttctgat ttgctttccc aggctgaaca cggcccggac tcacaggtga
    16081 ttttactgac gcccgctgct gatatggcgc gtcgcgtagc cgaagctgtc gaacgcctgc
    16141 tggcagaact gccgcgagct gaaaccgccc gccaggcact gaacgccagc cgcctgatcg
    16201 tgactaaaga tttagcgcag tgcgtagaga tctccaacca gtacggcccg gagcacctga
    16261 tcattcagac ccgcaacgcc cgcgatctgg tcgatggcat caccagcgcc ggttcggtat
    16321 ttcttggtga ctggtcaccg gaatccgcag gtgattacgc ctccggcacc aaccacgttc
    16381 tgccgactta cggttacacc gccacctgtt ccagcctcgg gctggcagat ttccagaagc
    16441 gtatgaccgt gcaggaactg tcgaaagaag gcttctccgc gctggcttca accattgaaa
    16501 cactggccgc cgccgagcgc ctgaccgccc ataaaaatgc cgttactttg cgtgttaacg
    16561 cccttaagga gcaagcatga gcaccgtgac tatcaccgat ttagcgcgtg aaaacgtccg
    16621 caacctgacg ccgtatcagt cggcgcgtcg tctgggcggt aacggcgacg tctggctgaa
    16681 cgccaacgaa taccccacag ccgtggagtt tcagcttact cagcaaacgc tcaaccgcta
    16741 cccggaatgt cagccgaaag cggtgattga aaattacgcg cagtatgcag gcgtaaaacc
    16801 ggagcaggtg ctggtcagcc gtggcgcgga cgaaggtatt gaactactga ttcgcgcttt
    16861 ttgcgaacca ggtaaagacg ccatcctcta ctgcccgcca acgtacggca tgtacagcgt
    16921 cagcgctgaa accattggcg tcgagtgccg cacagtgccg acgctggaca actggcaact
    16981 ggacttacag ggcatttccg acaagctgga cggcataaaa gtggtctatg tttgcagccc
    17041 caacaacccg accgggcaac tgatcaaccc gcaggatttt cgcactctgc tggagttaac
    17101 ccgcggtaaa gcgattgtgg ttgccgatga agcctatatc gagttttgcc cgcaggcatc
    17161 gctggctggc tggctggcgg aatatccgca cctggctatt ttgcgcacac tgtcgaaagc
    17221 ttttgctctg gcgggccttc gttgcggatt tacgctggca aacgaagaag tcatcaacct
    17281 gctgatgaaa gtgatcgccc cctacccgct ctcgacgccg gttgccgaca ttgcggccca
    17341 ggcgttaagc ccgcagggga tcgtcgccat gcgcgaacgg gtggcgcaaa ttattgcaga
    17401 gcgcgaatac ctgattgccg cactgaaaaa aatctcctgc gtggagcagg tttttgactc
    17461 tgaaaccaac tacattctgg cgcgctttaa agcctccagc gcagtgttta aatctttgtg
    17521 ggatcagggc attatcttac gtgatcagaa taaacaaccc tctttaagcg gctgcctgcg
    17581 aattaccgtc ggaacccgtg aagaaagcca gcgcgtcatt gacgccttac gtgcggagca
    17641 agtttaatga gtcagaagta tctttttatc gatcgcgatg gaaccctgat tagcgaaccg
    17701 ccgagtgatt ttcaggtgga ccgttttgac aaactcgcct ttgaaccggg cgtgatccca
    17761 gagctgctga agctgcaaaa agcgggctac aagctggtga tgatcactaa tcaggatggt
    17821 ctgggaacac aaagtttccc gcaggcggat ttcgatggcc cgcacaacct gatgatgcag
    17881 atcttcacct cgcaaggcgt acagtttgat gaagtgctga tttgcccgca cctgcccgca
    17941 gatgagtgcg actgccgtaa accgaaagta aaactggtgg agcgttatct ggctgagcaa
    18001 gcgatggatc gcgctaacag ttatgtgatt ggcgatcgcg cgaccgacat tcaactggcg
    18061 gaaaacatgg gtattaatgg tttacgctac gaccgcgaaa ccctgaactg gccgatgatt
    18121 ggcgagcaac tcactaaacg agaccgttac gcccatgtag tgcgcaacac caaagagacg
    18181 cagattgacg ttcaggtatg gctggatcgc gaaggtggca gcaagattaa caccggcgtt
    18241 ggcttctttg atcatatgct ggatcagatc gccactcacg gcggtttccg catggaaatc
    18301 aacgtcaaag gcgacctcta tatcgacgat caccacaccg tcgaagatac cgctctggcg
    18361 ctgggcgaag cgctaaaaat tgccctcgga gacaaacgcg gtatttgccg ctttggtttt
    18421 gtgctgccga tggacgaatg ccttgcccgc tgcgcgctgg atatctctgg tcgcccgcac
    18481 ctggaatata aagccgagtt tacctaccag cgtgtgggcg atctcagcac tgaaatgatc
    18541 gagcacttct tccgttcgct ttcatacacc atgggcgtga cgctacacct gaaaaccaaa
    18601 ggtaaaaacg atcaccaccg tgtagagagc ctgttcaaag cgtttggccg caccctgcgc
    18661 caggccatcc gcgtggaagg cgacaccctg ccctcgtcga aaggagtgct gtaatgaacg
    18721 tggtgatcct tgatactggc tgcgccaacc tgaactcggt gaagtctgcc attgcgcgcc
    18781 acggttatga acccaaagtc agccgtgacc cggacgtcgt gttgctggcc gataaactgt
    18841 ttttacccgg cgttggcacc gcgcaagcgg cgatggacca ggtgcgtgag cgcgagctgt
    18901 ttgatctcat caaagcctgt acccaaccgg tgctgggcat ctgcttaggg atgcaacttc
    18961 tggggcggcg cagcgaagag agcaacggcg tcgacttgct gggcatcatc gacgaagacg
    19021 tgccgaaaat gaccgacttt ggtctgccac tgccacatat gggctggaac cgcgtttatc
    19081 cgcaggcagg caaccgcctg tttcagggga ttgaagacgg cgcgtacttt tactttgttc
    19141 acagctacgc aatgccggtc aatccgtgga ccatcgccca gtgtaattac ggcgaaccgt
    19201 tcaccgcggc ggtacaaaaa gataacttct acggcgtgca gttccacccg gagcgttctg
    19261 gtgccgctgg cgctaatttg ctgaaaaact tcctggagat gtgatgatta ttccggcatt
    19321 agatttaatc gacggcaccg tggtgcgtct ccatcagggc gattacggca aacagcgcga
    19381 ttacggtaac gacccgctgc cacgcttaca ggattacgcc gcgcagggtg ccgaagtgtt
    19441 gcatctggtg gatctgaccg gggcaaaaga tccggctaaa cgtcaaatcc cgctgattaa
    19501 aaccctggtc gcgggcgtta acgttccggt gcaggttggc ggcggcgtgc gtagcgaaga
    19561 agacgtggcg gcgttactgg aagcgggcgt tgcgcgcgtg gtggttggct ccaccgcggt
    19621 gaaatcacca gaaatggtga aaggctggtt tgaacgcttc ggtgccgatg ccttagtgct
    19681 ggcgctggat gtccgtattg acgagcaagg caacaagcag gtggcagtca gcggctggca
    19741 agagaactcg ggcgtttcac tggaacaact ggtggaaacc tatctgcccg tcggcctgaa
    19801 acatgtgctg tgtaccgata tctcgcgcga cggcacgctg gcaggttcta acgtctcttt
    19861 atatgaagaa gtgtgcgcca gatatccgca ggtggcattt cagtcttccg gcggtattgg
    19921 cgacattgat gatgtcgcgg ccctgcgtgg tactggcgtg cgcggcgtaa tagttggtcg
    19981 tgcattactg gaaggtaaat tcaccgtgaa ggaggccatc gcatgctggc aaaacgcata
    20041 atcccatgtc tcgacgttcg tgatggtcag gtggtgaaag gcgtacagtt tcgcaaccat
    20101 gaaatcattg gcgatattgt accgctggca aaacgctacg ctgaagaagg tgcagacgaa
    20161 ctggtgttct acgatatcac cgcttccagc gatggccgtg tggtagataa aagctgggta
    20221 tctcgcgtgg cggaagtgat cgacattccg ttctgtgtgg cgggtgggat taagtctctg
    20281 gaagatgccg cgaaaattct ttcctttggc gcggataaaa tttccatcaa ctctcctgcg
    20341 ctggcggacc cgacgttaat tactcgcctg gcggatcgct ttggcgtgca gtgtattgtg
    20401 gtcggtattg atacctggta cgacgctgaa accggtaaat atcatgtgaa tcaatatacc
    20461 ggcgatgaaa gccgcacccg cgtcactcag tgggaaacgc tcgactgggt agaggaagtg
    20521 caaaaacgcg gtgccggaga aatcgtcctc aacatgatga atcaggacgg cgtgcgtaac
    20581 ggttacgacc tagaacaact gaaaaaagtg cgtgaagttt gccacgtccc gctgattgcc
    20641 tccggtggcg cgggcaccat ggaacacttc ctcgaagcct tccgcgatgc cgacgttgac
    20701 ggtgcgctgg cagcttctgt attccacaaa caaataatca atattagtga attaaaagcg
    20761 tacctggcaa cacagggcgt ggagatcagg atatgttaac agaacaacaa cgtcacgaac
    20821 tggactggga aaaaaccgac ggactgatgc cggtgattgt acaacacgcg gtatctggcg
    20881 aagtgttaat gctgggctat atgaacccgg aagctctgga caaaactatc gaaagcggca
    20941 aagtcacctt cttctcgcga actaaacaac ggctgtggac caaaggcgaa acgtctggca
    21001 attttctcaa tgtggtgagt attgccccgg actgcgacaa cgacacgtta ctggtgctgg
    21061 cgaatcccat cgggccaacc tgccacaaag gcaccagcag ctgcttcggc gacaccgctc
    21121 accagtggct gttcctgtat caactggaac aactgctcgc cgaacgcaaa tctgccgacc
    21181 cggaaacctc ttacacagcc aaactgtatg ccagcggcac caaacgcatt gcgcagaaag
    21241 tgggcgaaga aggggtggaa accgcactgg ccgcgacggt aaatgaccgc tttgagctga
    21301 ccaacgaggc atctgatttg atgtatcacc tgctggtgct gttgcaggat caggggctgg
    21361 atctcggaga agtgattgat aatctgaaga atcggcatta acgcgttggc ccccgcagca
    21421 acggcggggg tagcagctct caggatttgc tttccagcaa tgtagtgtag agattgacat
    21481 aggcgtcaat catatggtcg gcagtaaaca tctcttcaaa acgacgacgc gagttttcgc
    21541 catagcggtt ggaggtttcc tcgttattcc agagctcatt catcgcctcc accagcgcct
    21601 ggctatcatt cggcggaatc acgcaaccac tcactttgtc ctggttaatg aaagaggtac
    21661 ctgtaccgat ctcgcaagag atcagcggcc ttgcaaagcg tgcgccttcc aataacgtaa
    21721 tgccaaacgc ctcagagcgc agatgcgagg ggaataccac gccccggcag agctggaaga
    21781 gaatgtactt atcttcgtcg ttgagcatgc cggtaaacac cacattgctc agcccgcgtt
    21841 gctgcgcctc acgccgcact tccgcctcca gcggcccgcc cccgacgatc accaccggca
    21901 gccggctacg ctcggcggca tccagcagaa tgtgcagccc tttgtagtag cggaaagcgc
    21961 cgacgaagag gaagaagtta tcgccgacgg tttcccgcca gtgcgccacc cgctgcggat
    22021 cgtgctgcac gtcatgctgc tccagaccaa acgggatcac cacggtttta tcctgatatt
    22081 ttttcagggt ctggctggag gccacgtagt tgggcgacga ggcgacgatg cagtctacgc
    22141 tggcgaggaa tcgctcctgc agcggctggt acaacttcat taaccgtttt tgtttcacaa
    22201 tatccgagtg ataggtcacc accgttcttg cgtcaggccg cgccgagaga tgcaacatat
    22261 ccatgaaagg aaacggaaaa tggtagttga tgatgtcgta atcatcttta atctgcttaa
    22321 aacgctttaa cgctttcaac gaaaacggcg tggaggcaat ttcaaaaagc tgcttattat
    22381 aaatgacccg atggtcttcg taggcgacag gacctgtctc tttgtccggg ctaaaagtaa
    22441 aaacatcggc tgcgattccc cggcgggcgc agccctgact tagctgataa atgacctgct
    22501 caataccgcc gtaggtatcg ggatagtagg tcttatagac gtgtagaact ctcaactgac
    22561 acccttagag taatttatag gcgttaatgg tctgggtcgt acagttctcc cacgaaaact
    22621 gtttcgcctg ggcaagaccg cgcgcggtgg cgatttcccg ccagctatca tcctgcaggc
    22681 tctgcaaaat atgtgcgcta atcgcgtcta catcattagg atcggcaacg aggccggcat
    22741 cgccaaccac ctcaggcaaa gaagtgacat tggaacatac taccggcaca ccgcaagaca
    22801 tcgcttcaag aataggtaaa ccgaatccct catagaagga gggataaaca aaggttctgg
    22861 ccgccgcgta cagataaggc aggtcttcat ccgggacata gcccaggtaa cggatccacc
    22921 cttcacgcgt accgcgctcg actaactgcc acagcacatc gtcttcccat ccgcgataac
    22981 cgctgaggat cagcgggtag cgcatgcggg tctccatcgg cagcagctga taggcctgca
    23041 gcagaccgcg gatattttta cgcggctcca tggtgccgat atataacgca tacccctgcc
    23101 acgccagctg atatttctgc aggaccggca ggcactccgc cgggctgcgc ggaatatagt
    23161 cgctgctgca ggccagcttg gtggtgacga tccgatccgc cggatagttg aacaggcgga
    23221 tgatttcatt gcgcgagaag tcggagacgg tcaggatcag cttcgccgaa tccaggctct
    23281 cgtgcaggga cttctccata tagcgaaccc gatcttttgg atgatattcc gggcaggtaa
    23341 aaatggagat gtcatgaaac gtggtcacgg cgcgttccag gcgatgcggc agatagaaat
    23401 tgggaccatg gtaaatgtag tctttgtaat cgcgcaatgc ccacgcctgg cgccgcggat
    23461 gcagcaggcg atacgcctca atcagcagcg gctggcggcg cagaaacgcc gacagacgac
    23521 catgattgct ggctttgcta tcgcttttat tctccacctg ggggatctgg tcgataaacg
    23581 acgcgccgtg aaacagcttc agctcttcga tttcacgggc aaccgccagc cgcttaacca
    23641 gctccaggga ataccgaccg atgcccgtta atgggtattt aattggctca gtagcaaaaa
    23701 taattttcat aacgaactca tcacaagggc caccgcagta gccctgttga tagcgattat
    23761 tgcttcgcgg ctggcgcgat aatcggcagg tttttcagga gaaattccgc gctctgtttc
    23821 caggttaacc agttgatatt ttcggaacga ggatgcgcgt tttgctcgta cagggctaac
    23881 cactcttcta ccgccttcgc gatatcggac ggcgcttcac cggagaaata ccaggcatgt
    23941 tcctgagcaa tctctttaaa caccggaatg tcgcgaataa tcaccggcag ctttttctgc
    24001 gccgcttcaa tcaacggcag gccaaagcct tctccctgcg aggcaaagat cagcgcgcgt
    24061 gaacgagcgt atagctcggc caaaaactcg tcgctgatat tctgcagcca gaagagcttt
    24121 ttgttcagct ggggatgatg gcgtaatttt tcgcacaagc tgtcaacgtt ccagccctgt
    24181 ttgccgacga taaacaagtt gtagtctttg ccctcgcgcc acagttcctc gaaggcggcc
    24241 agcgtctggg cgtggccttt gcgcggctcc atggtaccca ccatgataaa tgacggagct
    24301 gcagccatcg ctgctaacag cgcctgtgca ttatccggca tgccagtgga cggtacgctg
    24361 gcatcgaggt cggctcccag atggaagttg ctgacggtct gcagcgggtt cggtttcacc
    24421 caatggcgat tttccgcaat ccacgctttc acatcctcgg cgacgctggc agagacgcag
    24481 atcagccggt cggcgtgctc tgcgaggcag gacagccaga tcgggaaatc acgctgaatg
    24541 ccttcgatac tccactccgg acggcgtaat ggcagaatat catgcaccac gaagcaggcg
    24601 aacgccccgg cggcgcgcat gctgtcaatt tgcgtcacca gctccgggaa gagatgcgcg
    24661 gtcagatcgg tggcaataag cacatcgtct ttgctgaaca gcaccggttc gtcagcacca
    24721 aattcgcccg ggaaatgact ggacagatat tggttggcgt agcgataaca ctcgccaggg
    24781 gtgtagtaga cggcggaaac ctcgtaaccc ggcacgccag atttcagtaa ttcgctgagg
    24841 atactgcgcg agacgcgctg gatcccggtc ttcgcgtcat gctgcgccag aaccgaaatg
    24901 tccaccagca gcttgcgggt atggcgctta aagctattgc gcgcaaccgc ccacgccaga
    24961 cctagacggt cgacttcgct cagcgcatcg attttctgca tcatctgaag gcgggaggcg
    25021 gtcacgcttt ccgcctgccc ggcatccgtc tcttgggacg actgcagcag acgagtgaaa
    25081 ccctcgatcg ccagatgggc ggtattggcc cacgagaatt tggccgactg cgcgagagca
    25141 tgcgccttca gctcacgata aaacgcctca tcggtcagcg ccttctccat gacccggcga
    25201 atgtcctgca catcatgcgg attgaacatg gcatcttccc agccaatgac ttccggcagg
    25261 ctggtaatgt ttgaacctaa ggtcgccgcc ccgcagcgca tcgcttccag cggcggcagg
    25321 ccgaaacctt catgcagcga cgggaacaca aacagtttgc acaggttgta cagggcaatc
    25381 agatcgtcgt cggtcaggaa cccagtaaag ataagctggc tgcgtgacaa accatagctt
    25441 tccgccagac gcaggatccg ctccagttgt tctggctgca ctttatacgc cagcaccatc
    25501 ggatagcgct gctgcagctc ggcaggcagc aagctgtagg cgtgaataag cgcttcaata
    25561 tttttccgcg gctcgatcat cgccagcgac aagataaact catccggcag gctgtatttg
    25621 tcttttaaag actgaatacg ctcagcggaa taatcgatca ccgcaaactc ttcgcccacc
    25681 gccgaggaga tgttcagtac ccgatcgctg gcgatgtcgg tatattcgat cacttcctga
    25741 gctgccgact gcgaaatggc gaagatcgcg tctgcccgct caaattccgc cagcttattc
    25801 atataaaact cacggaaatt cggatcgccg agataacgct ctttatttaa cagtgggatc
    25861 agatcgtggc atacgcacac cgttttccac ggcacattat ccgccggaat agacacggta
    25921 taactatcgc tatgcccctc gaagaagcta ataacataga cgatatcagg cgcgatattg
    25981 gcgatagcga tatcgcgggc cgcctgggcc gctttactgc ggccatggtt ttcaatatga
    26041 cgataggctg ttggcgcgac ggctgagaag atgaacatat cttcatccgt cagcaggtcc
    26101 cgataggcca ttttaacatc attaatatta tcaatgggat acataccatt gatcaaaata
    26161 ctgacgcgat gctcaccggc attttcaata atggcgcggc tcatggccag ggtactgcga
    26221 ccaataccgc ggactttact ttccgactgg taaccctgga catcaatcaa aatatgcaag
    26281 ttaatctcct tatttatttt tcgttagttt gagataaatt tcataaacca cgggaggtaa
    26341 aagttccgga tgcatcactt gcagttcagt ctgcgataaa atctgcgcat cagcctgata
    26401 ctggctgtgc ggcagtggat tcatacgacg atacaaacga taggccggct gataaagccc
    26461 cagcttatgt aaaccattta ccgtggtatg ttttacctgc ggatgttttc tcataaatga
    26521 gaaaacaaac cgcaggacgc gcttaataaa atgtttacac cgctgcacga aaccatactg
    26581 cctcagcaaa tggatctgca ggcccagata acgataaccc ttggtcattc tccaggaacg
    26641 gctgcgatgg attttttcaa tttcctggcg aagctgttgg ttttcgttgc ttaaatggtg
    26701 gatctcccgg ttagcggctt ccagtcgctg cagtagctca gcggtttcag ggggcaactc
    26761 gggctgaacg tcagcctggg cgtgcgcttg cgccgtctgc aacagcgccg ccagttgctc
    26821 catattctca ccaagccagg tgagttcatg gctgacttcc actggctgtt gagctttctc
    26881 gacgaacgca tcctgagcgg actgcagctg agccatctgc tgctccaggg tatggatttg
    26941 gccgcgcaac gcttctgagc cggcgctttc atttagcacc cgggactgga gctccagcag
    27001 caccgtctcc tgagcgatga tccactgttc cgtcgccccg cgctgctgtt cagctgacgg
    27061 cagcttcgct ttcttctcga acagcgccaa cagcagggca aagttccagc gttcaaccgg
    27121 ctcctgccag accgcataca gccagttaga ccacggctga ggcagattaa acggatgaac
    27181 aggcgccgag cgccagaaac cgttccagct tttgttttcg gcaaacagct cattcacaaa
    27241 cacgaagaac gactggacaa ggtttgccgg ccagctgcag tcctgtggcg tggtgacgat
    27301 agaaccaaag tcgattaagc gggcatgctg ccgtgcatcg accatcacgt tccacgggcg
    27361 cacatcgtca tgccataacc cttgcttctc cagcaccgct aaggaacgga gcaaggtgcc
    27421 aatgattttt tcacgatcga tctcttcccc ggccaccagc atatcgctca gcaagctacc
    27481 cgggagtttt tccatcacca gccaaccaga ctgggcattt tcgccgtggg ctagtaatgc
    27541 cggcgtgtca aagcccgtag ggggttgtgc cagaaaacac atttcattat gcagttcgtt
    27601 tttattacgc tggctctctt ccgccgtcag gatgccgtgg ggcatgtcgt aatagaaaaa
    27661 cttgcataca taatcttcgc caaagaagta gcgacggctg cgcttatgcg ccaggccggc
    27721 cccggcatag ggctgattct gccagtgctg gaacggctga ttaaaatcgt tgatcagcac
    27781 ccggtggttg ctgaccaggt acatcgggcg cggtacgggg gataaatggg tgtcaaactc
    27841 accgatcagg cgataaaacg cgcactgctc aatcagttcg cgcgggtcgt ccggctgaga
    27901 aacgccccag taaagcggtt cttctttcac cgccagctca aggatcaccg cctgggtgac
    27961 atcggccagg cgcgagagca gacgtttaac ttcatcgata ccatgaaggt gaacaatatg
    28021 gtgaaacacg cttaagccaa tggccagatc aaactcgccc tcttccagcg cggcgatcac
    28081 ctcttcaatt ctgcctaccc ggaactcggc ggcaaaatcg gggttctctt cggccagcgc
    28141 gcggcacacg ttaatatttt cctgctggaa atcgatacca acgatagtcg cgcctttgct
    28201 tgccagactc aggctaaaaa agccctgcgc gcagcccaga tcgagaacgt taagcgggcg
    28261 ccccagcgca cgggataagt tatcgtactg ttcggtaatc aaatcgaggc gttgattaca
    28321 atcacgagcg gcgtcgccat cccattcggg atggccaaaa atagtttgat aaatctcagg
    28381 taattcgcta accagcgtgt ttaagtcttt agtcatggaa tcgtttttct caacgtcatt
    28441 ggtagctagt aaaggacgac ttaagatcga tgacacccca gaaatccatg ctcttggtca
    28501 catgtaaaat aatggagtcg taacgtctgt ccagcggcgt catatccgtc tgcgggttgc
    28561 cggcagagat accaaatgac agcaggtaat cgccactatt taaatcaacc ggaaggctaa
    28621 atttaaagac ccgaacatcg ccgcgggaaa ccgaaatatt ttcccgccct tcagaggcga
    28681 ggaaagagtt ggttccgtag aggaataagc catcgagggt cttaatcagg atgcctggca
    28741 ccacgcaatc aaaatcgtat tcaaacacga ccttcatcag aaaatcggtc tgctgattgc
    28801 ccgtcagtga gggaggaaaa tcaaccccgg cgctctggat atgataatcg atgattttcg
    28861 cgccaccctg cccccagcga tattcttccg gacggtagcc tgggcgcgtg tggtacacat
    28921 cttcaatctc atcgagagac atctggcttt cgccactggc tgacgagata gccgttgcgc
    28981 tttttgtcgc gctgtcttta tccggtttgc caaacagctc atccaggtaa cggttggtta
    29041 cgtcgcgggc ggtgccgtcc atgcagatgt caccattttt gaggaaaatg gcgcggtcgc
    29101 agtgcttgac gatatccccg gcgctgtggg aaaccagcag cagcgtggtc ccctgctctt
    29161 tgaagtccgc gatgcgagca tagcatttcg cctgaaaacg ggagtcaccc acggagagcg
    29221 cttcatcgac gatcagaata tccggccggg aggccgtggc gacagcgaac gccaggcgca
    29281 tttgcatccc gctggaatag atgcgtacgg gctcttcaat gtagtcgccg atatcggcga
    29341 aggcttcgat ctccggcatt aagcgctcga tctcttcccg gcccaggccc atcatcagcc
    29401 cggacatata cacgttctgc cggccggtaa agtcaggatg gaagcccatg cccagctcca
    29461 gcagcgcggc gacgcgcccc tggatttcaa tgctaccttt ggtgggctga gtggtgccag
    29521 tcagcagctt cagtaacgta cttttacctg cgccgttcac cccaacaatg ccgaccgctt
    29581 ccccaggttc aatgctgaaa ttgatatctt tcaataccca tttcttgctg tgccgcggct
    29641 tatcgcccgg cagtagtttc tcaatgaccc ggttccattt agatggataa tatttatacg
    29701 ccttgccgac gtgctgaact ttgatactca tgattaaatc tcgtccacaa tatcagcggc
    29761 atgtttttta aataaacgcc aggcaaataa aaacagaatg accgacacca ctgtgaccgg
    29821 aagcagcgcc atccagttag ggctctggtg atagagcatc acgttctggt aggaaccgat
    29881 aatggtcgcc atcgggttat acgccaacag accagaaacc cattccggca gcgttttgga
    29941 tacgtagaca attggcgtaa accagaacca aaactgaagc aggatattaa cgaactgccc
    30001 gacatcgcgg acaaaaacgt tcagtacccc gagaatgatc ccgaggccga gggtgaacag
    30061 catctggacg atcagcaccg gaatgatttc aaagaaaatc atgccaggga aattgcccgt
    30121 gacgatcaga aacagcacga acagaccaaa aatgatcagg aagttaataa atgccgaagc
    30181 agtgacgata atcggtaaac agatccgcgg aaagctgagc ttcttaagaa tattggcgtt
    30241 ggtcagaaaa acgttgacca gactgttgag catttccgtg aacagccccc aagttaacac
    30301 cccagaacac aagtagatgc tgtatgcaaa aggaccatcc atgccagcca ggcgggcacg
    30361 cattaattca gaaaaaatta atgtatagac ggaaatcatg gcgatcggct gtaaaatcag
    30421 ccatgccgcg cctaagaagc tggtttggta acgggactga aagtcccgtt taacgctgtc
    30481 aataataaag ccacggtagc gatagatcgc tgaaaacatc ggactcaaag caattcctct
    30541 tttaacaggt tgagcagctc ggtcgttttt tcattcataa gcgccacatc agctctggac
    30601 tcaacgttca gacgcaccac cggctcggtg ttagacgtgc gcaggttaaa gcgccattca
    30661 gggtattcaa tactgatccc gtccgttgtg tcgatgtgtg cagccgccgg ctcatactgc
    30721 gcccggatgc gcgctatcgc ctctgccgca ttacccagct tgcggttgat ctcccccgac
    30781 gccgggaacg ccgcctggcg gtccgccacc agcgatttca gcgagctgtt cttcaggcac
    30841 agcagctccg ccaccagcag ccacgggatc atcccgctgt cgcagtaggc gaaatcgcgg
    30901 aagtaatggt gcgcactcat ctccccgccg tagatagcgt cttcctgacg catccgctcc
    30961 ttgatgaacg catgccccgt cttcgacatc accggctggc cgccgttgcg ggtcacgatg
    31021 tccaccgtgt tccacgtcag gcgcgggtcg tgaatgattt tcgcccccgg ctgcttctgc
    31081 aggaacgcct ccgccagcag gccgacaatg tagtacccct cgataaacga cgcctcgtca
    31141 tcgaacagga agcagcgatc gaagtcgccg tcaaaggcaa tccccatgtc ggcctgatgc
    31201 tcgcgcaccg cgtcggcggt atcctggcgg cactccggca gcagcggatt agggataccg
    31261 ttagggaaat ggccatccgg ctggtgatgc accttgataa aggttaccgg caccccggcc
    31321 gccgcgaagc gtttctccac ctcatcaatc acgtgccccg ccgccccgtt gccggagttc
    31381 accaccagct tcagcggacg ggtgaagttc gccaggtcca cgtagcccat caggtggtcg
    31441 acgtactcct tcagcaccga aatctgacgc agggtcccgc gacgtgccgg gtccaccggc
    31501 gggaactggt tctcctccgc caggcgctgg atatcccgca ggccggtgtc gccgctgatg
    31561 ggcttcgcgt tctcgcgcac cagcttcatg ccgttgtagt tcatcgggtt gtggctcgcc
    31621 gtcacctcga tgccgccgtc caccccgagg tggaaggtgg cgaagtaaat ctcttccgtg
    31681 ccgctcagtc cgatgtccag cacatcggta ccggcgtcca ttaacccgcg ggccagcgcc
    31741 agcttcaacg actcgcttgt gaggcgcaca tcccccccca ccactatctt cccgggtttc
    31801 agaaattcgc cgtaggcgcg cccgatacgg taggcgatgt cctcgttcag ttcctcaccc
    31861 agttcaccac ggatgtcata agccttaaaa caagttaact gtgtcattct gcatcttgtc
    31921 ccgaaaataa ttagcaacga ccatactggt ctttaatacg aataatgtcg tcctcgccga
    31981 ggtacgaccc cgactggatc tccagcactt ccagcggaat acagccaggg ttttccaggc
    32041 agtgctcggc gccaatcgga ataaaggtgg actggttctc ggacaacagg aactgcttac
    32101 cgttgaccgt cacctgaccg gtgccggcga gaataaccca atgctcagcg cgatggtggt
    32161 gcatctgcat cgagaaggca ccgccaggtt tcaccgtgat gcggttgacg ttgaagcgcg
    32221 gggtctggac caccacgtcg cagcggcccc agggacggta aatctcacgg tggcgcttgt
    32281 actcgctgcg ctggttctgc ttgaggaact cgaccgcctt cttcacgtcc tgggagcgct
    32341 tgcggttcat caccagcacg gcgtccttgg tgctgacaat caccaggttc tccacaccga
    32401 tggccgccac cagcttctcg tcgctgttga tgtagcagtt ttcgctgttg tgcacccacg
    32461 cgtcaccgct gaggacatta ccctgcccgt ctttcgggct gacctcccat agtgcggacc
    32521 aggagccaac gtcgctccag tcagcatcga gaccgaccac caccgcatca gcggttttct
    32581 ccataacggc gtagtccacg gactcgtccg ggcactcgca gaaaatatca tgtgggatat
    32641 tgatgaaatc gctgccatta tcggcggcat tcaccgcagc ctggcaggtt tccaggatat
    32701 ccggacggta tttggccagc tcagagaggt attttttggc acggaacata aacatgccgc
    32761 tgttccagta gtactccccg gaggcgaggt agacctcggc gcgctcgcgg tccggcttct
    32821 ccacgaagcg ggccacctgg tacggggcgt gggcgctgtc ggtgagcgcc acgccgcgct
    32881 ggatgtagcc gtagccggtt tccggggcgt tcggcacgat accgaaggtg accagatggc
    32941 cttcatcggc atactgctcg gcgacgcgga tggcgtcgtg gaaggccgac tgattgttga
    33001 tgatatggtc ggcggcgagg accagcatca gcgggtcgtc accgtagcgg gtggcctgaa
    33061 gggccgccag ggcgatggcc ggcgcggtgt tgcgcccgac cggctcaagt ataatgttat
    33121 tcgacagctt attgagctgg cgcagctgtt cagccaccag gaaacgatgc tcttcgttac
    33181 agatgaccat cggttcatgg atttcaaggc ccgagagtcg ggtaatggtt tcctgcagca
    33241 tggagttctg cccgaacaga cggaggaact gtttcgggta aagctcgcga gacatcggcc
    33301 agagacgact gccggtaccg ccagccataa ttacaggaag taacataatg agatccttaa
    33361 ataaataatt gctttatttt actatcaatc aatcgaccgt caggtggcgt ggcctttttg
    33421 aggcacgcta ccgccattgg ctgtcgcaac caaaatgcgt actgctcccg tgctccaggc
    33481 taagcgccac cctctttcgc ttcaggctgt cggcagatcg ttcagtcagc agctacaatg
    33541 acagcctggc ggaatgcgtg tcctgaaaac ctaaaagcag agatatacct tcaggcatat
    33601 ctctgctttt aacatgcatc ttccctcacc tggccgccaa acgcaccaca ctttccgcat
    33661 ttgccgccgg gttaaccatc aggactttct catttccgca gggcaaaaca catgccctta
    33721 ccgccaccga agaaaacgaa accggcggtt taccgatgcc agtaaccgtc atagaggggc
    33781 attcacgata aagggaaaga tgagaagaga catcgtcttc gccgctgagg agacaacgcg
    33841 cttgcgggcc tggttgccct ttgcacatgg cgacggcgct acgttccgac aggcgggata
    33901 aacgactcat gctctacaat gcacattcca ctaagaaaat cgtccgcaga ttatagcgct
    33961 ggtcgcgggg aataaaaata aaaaagccta ctgattaatg gattactcaa ataataca
//
LOCUS       DAJOSY010000042        33809 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJOSY010000042 DAJOSY010000000
VERSION     DAJOSY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.042"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            172..1230
                     /gene="citC"
                     /locus_tag="OHN27_002091"
     CDS             172..1230
                     /gene="citC"
                     /locus_tag="OHN27_002091"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCQ2286530.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1245..1541
                     /gene="citD"
                     /locus_tag="OHN27_002092"
     CDS             1245..1541
                     /gene="citD"
                     /locus_tag="OHN27_002092"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCQ2286531.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1538..2446
                     /gene="citE"
                     /locus_tag="OHN27_002093"
     CDS             1538..2446
                     /gene="citE"
                     /locus_tag="OHN27_002093"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCQ2286532.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2457..3989
                     /gene="citF"
                     /locus_tag="OHN27_002094"
     CDS             2457..3989
                     /gene="citF"
                     /locus_tag="OHN27_002094"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCQ2286533.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3993..4544
                     /gene="citX"
                     /locus_tag="OHN27_002095"
     CDS             3993..4544
                     /gene="citX"
                     /locus_tag="OHN27_002095"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCQ2286534.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDCEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4519..5397
                     /gene="citG"
                     /locus_tag="OHN27_002096"
     CDS             4519..5397
                     /gene="citG"
                     /locus_tag="OHN27_002096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCQ2286535.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5448..6911
                     /gene="citT"
                     /locus_tag="OHN27_002097"
     CDS             5448..6911
                     /gene="citT"
                     /locus_tag="OHN27_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCQ2286536.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7025..7831
                     /gene="rna"
                     /locus_tag="OHN27_002098"
     CDS             7025..7831
                     /gene="rna"
                     /locus_tag="OHN27_002098"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCQ2286537.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8061..8471
                     /gene="rnk"
                     /locus_tag="OHN27_002099"
     CDS             8061..8471
                     /gene="rnk"
                     /locus_tag="OHN27_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCQ2286538.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8702..9940)
                     /locus_tag="OHN27_002100"
     CDS             complement(8702..9940)
                     /locus_tag="OHN27_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCQ2286539.1"
                     /translation="MKALTYHGPHHVQVENVPDPGVEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLHFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10161..10589
                     /gene="uspG"
                     /locus_tag="OHN27_002101"
     CDS             10161..10589
                     /gene="uspG"
                     /locus_tag="OHN27_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCQ2286540.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10710..12275)
                     /gene="ahpF"
                     /locus_tag="OHN27_002102"
     CDS             complement(10710..12275)
                     /gene="ahpF"
                     /locus_tag="OHN27_002102"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCQ2286541.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTV"
     gene            complement(12520..13083)
                     /gene="ahpC"
                     /locus_tag="OHN27_002103"
     CDS             complement(12520..13083)
                     /gene="ahpC"
                     /locus_tag="OHN27_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCQ2286542.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13455..14201
                     /gene="dsbG"
                     /locus_tag="OHN27_002104"
     CDS             13455..14201
                     /gene="dsbG"
                     /locus_tag="OHN27_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCQ2286543.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14410..15312
                     /locus_tag="OHN27_002105"
     CDS             14410..15312
                     /locus_tag="OHN27_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2286544.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15459..16679
                     /locus_tag="OHN27_002106"
     CDS             15459..16679
                     /locus_tag="OHN27_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCQ2286545.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPDGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKSKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            16652..17281
                     /locus_tag="OHN27_002107"
     CDS             16652..17281
                     /locus_tag="OHN27_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCQ2286546.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(17282..18442)
                     /gene="ybdL"
                     /locus_tag="OHN27_002108"
     CDS             complement(17282..18442)
                     /gene="ybdL"
                     /locus_tag="OHN27_002108"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCQ2286547.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAHHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            18551..19639
                     /gene="hcxA"
                     /locus_tag="OHN27_002109"
     CDS             18551..19639
                     /gene="hcxA"
                     /locus_tag="OHN27_002109"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCQ2286548.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESNVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFKKVESFKA"
     gene            complement(19649..19846)
                     /locus_tag="OHN27_002110"
     CDS             complement(19649..19846)
                     /locus_tag="OHN27_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCQ2286549.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(20028..22133)
                     /gene="cstA"
                     /locus_tag="OHN27_002111"
     CDS             complement(20028..22133)
                     /gene="cstA"
                     /locus_tag="OHN27_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCQ2286550.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLIESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(22314..22727)
                     /gene="entH"
                     /locus_tag="OHN27_002112"
     CDS             complement(22314..22727)
                     /gene="entH"
                     /locus_tag="OHN27_002112"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCQ2286551.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(22730..23476)
                     /gene="entA"
                     /locus_tag="OHN27_002113"
     CDS             complement(22730..23476)
                     /gene="entA"
                     /locus_tag="OHN27_002113"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCQ2286552.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(23476..24333)
                     /gene="entB"
                     /locus_tag="OHN27_002114"
     CDS             complement(23476..24333)
                     /gene="entB"
                     /locus_tag="OHN27_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCQ2286553.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(24347..25957)
                     /gene="entE"
                     /locus_tag="OHN27_002115"
     CDS             complement(24347..25957)
                     /gene="entE"
                     /locus_tag="OHN27_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCQ2286554.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALIEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(25967..27142)
                     /gene="entC"
                     /locus_tag="OHN27_002116"
     CDS             complement(25967..27142)
                     /gene="entC"
                     /locus_tag="OHN27_002116"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCQ2286555.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            27517..28473
                     /gene="fepB"
                     /locus_tag="OHN27_002117"
     CDS             27517..28473
                     /gene="fepB"
                     /locus_tag="OHN27_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCQ2286556.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF"
     gene            complement(28477..29727)
                     /gene="entS"
                     /locus_tag="OHN27_002118"
     CDS             complement(28477..29727)
                     /gene="entS"
                     /locus_tag="OHN27_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCQ2286557.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPAVPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRHFRQTPPQVTASDS"
     gene            29838..30842
                     /gene="fepD"
                     /locus_tag="OHN27_002119"
     CDS             29838..30842
                     /gene="fepD"
                     /locus_tag="OHN27_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCQ2286558.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            30839..31831
                     /gene="fepG"
                     /locus_tag="OHN27_002120"
     CDS             30839..31831
                     /gene="fepG"
                     /locus_tag="OHN27_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCQ2286559.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            31828..32643
                     /gene="fepC"
                     /locus_tag="OHN27_002121"
     CDS             31828..32643
                     /gene="fepC"
                     /locus_tag="OHN27_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCQ2286560.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(32640..33773)
                     /gene="fepE"
                     /locus_tag="OHN27_002122"
     CDS             complement(32640..33773)
                     /gene="fepE"
                     /locus_tag="OHN27_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCQ2286561.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKSFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
BASE COUNT         8080 a         9232 c         8552 g         7945 t
ORIGIN      
        1 atcaaaattt cacgccgaaa tactccttag gatgtatagc gaaaggagaa aaagatatac
       61 ctcgatcacc cacctttctc ccaagtgaaa ataaaaagtt atcagtttgc aacattgaac
      121 aacattcgtt gcaaatcgat aacaacatgc accttcagga tactatttat tatgttcggc
      181 aatgatattt tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa
      241 ttcctgcatg aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc
      301 cgcgatgaaa agcttatcgc gtgcggtgga attgccggaa atattattaa atgcgttgct
      361 atcagtgaat ccgttcgcgg tgaaggactg gcgctgacat tagccactga actgataaac
      421 ctcgcctatg agcggcacag cacgcatctg tttatttata ccaaaaccga atacgaggcg
      481 ctgttccgcc agtgcggttt ttccacgctg acctgcgtac ccggcgtgat ggtgctgatg
      541 gaaaacagcg ccacgcgact gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca
      601 gggaacaaga ttggctgcat tgtgatgaac gccaatccct ttacgaatgg tcaccgttat
      661 ctgattcaac aggctgcggc acagtgcgac tggttgcatc tgtttttagt caaagaagat
      721 tcttcacgct tcccctatga agaccggctg gatctggtgt taaaaggcac cgccgatatt
      781 ccacgcctga ctgtgcatcg cggctccgaa tacatcatct cccgcgctac gttcccttgc
      841 tacttcatta aagaacagag cgtcattaac cattgttaca ccgaaattga tctgaagatt
      901 ttccgtcagt acctcgctcc cgcactgggt gtaactcacc gctttgtcgg tactgaaccc
      961 ttttgtcgcg ttaccgccca gtacaaccag gatatgcgct actggctgga aacgccgact
     1021 atctccgcac cgcccatcga actggttgaa attgagcggc tgcgttacca ggagatgccg
     1081 atatccgctt cccgggtacg tcaactgctg gcgaaaaacg atctcacggc tatcgcgccg
     1141 ctggtccctg cagtcacgct gcattatttg cagaacctgc ttgagcactc ccgccaggac
     1201 gcggcagctc gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc
     1261 ccgccgttgc aggcaccctt gagtctgggg atgtgatgat acgcatcgcc ccactcgata
     1321 cgcaggatat cgacctgcaa atcaacagca gcgttgagaa acagtttggc gatgcaattc
     1381 gcaccaccat tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg
     1441 atgacaaagg cgcactggac tgcattttac gtgcacgact ggaagccctg ctggcacgcg
     1501 ccagcggtat cccggctctg ccatgggagg attgccaatg atttccgctt cgctgcaaca
     1561 acgtaaaact cgcacccgcc gcagcatgtt gtttgtgcct ggtgccaatg ccgcgatggt
     1621 cagcaactcc ttcatctacc cggctgatgc cctgatgttt gacctcgaag actccgtagc
     1681 attgcgtgaa aaagacaccg cccgccgcat ggtttaccac gcgctgcaac atccgctgta
     1741 tcgcgatatt gaaaccattg tgcgtgtcaa cgcgctggat tccgaatggg gtgtcaacga
     1801 cctggaagcc gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc
     1861 tcaggatgtt ctggatatcg aaaaagagat cctgcgtatc gaaaaagcct gtggtcgtga
     1921 acccggcagc accggcctgc tggcggcgat tgaatctccg ctgggcatta cccgcgcagt
     1981 ggaaatcgct cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag aagactatgt
     2041 gcgcaacctg cgtacagaac gctccccgga aggaactgaa ctgctgttcg ctcgctgctc
     2101 cattttgcag gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct attccgacgc
     2161 taacaacgaa gctggattcc tgcaagaagc cgcccacatc aaacagctgg gctttgacgg
     2221 caaatcgctg atcaacccgc gtcagattga tctgctgcac aacctctacg caccgaccca
     2281 gaaagaagtg gatcacgccc gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg
     2341 cctcggcgtg gtttccctga acggcaagat ggtggacggt ccggttatcg atcgcgcccg
     2401 tctggtgctc tcccgtgcag aactttccgg catccgcgaa gaataaggca atcaaaatga
     2461 cgcagaaaat tgaacaatct caacgacaag aacgggtagc ggcctggaat cgtcgcgctg
     2521 aatgcgatct tgccgctttc cagaactcac caaagcaaac ctaccaggct gaaaaagcgc
     2581 gcgatcgcaa actgtgcgcc aacctggaag aagcgattcg tcgctctggt ttacaggacg
     2641 gcatgacggt ttccttccat cacgctttcc gtggcggtga cctgaccgtc aatatggtga
     2701 tggacgtcat cgcgaagatg ggctttaaaa acctgaccct ggcgtccagc tccctgagtg
     2761 attgccatgc gccgctggta gaacacattc gccagggcgt ggttacccgc atttatacct
     2821 ccggcctgcg tggtccactg gcggaagaga tctcccgtgg tctgctggcg gaaccggtgc
     2881 agatccactc tcacggcgga cgtgtgcatc tggtacagag cggcgaactg aatatcgacg
     2941 tggctttcct cggcgtcccg tcctgtgatg aattcggtaa tgccaacggc tacaccggta
     3001 aagcctgctg cggctccctc ggctatgcaa tggttgatgc cgacaacgca aaacaggttg
     3061 tgatgcttac cgaagaactg ctgccttatc cgcataatcc ggcaagcatt gagcaagatc
     3121 aggttgatct gatcgtcaaa gttgatcgcg ttggcgatgc tgcaaaaatc ggcgctggcg
     3181 ctacccgtat gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga
     3241 ttgtcaactc tggctacttc aaagaaggtt tctccatgca aaccggcacc ggcggcgcat
     3301 cgctggcggt aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact
     3361 tcgcccttgg cggtattacc gcgacgatgg ttgacctgca cgaaaaaggt ctgatccgca
     3421 aactgctgga tgtgcagagc tttgacagcc atgctgcgca atcgctggcc cgtaacccca
     3481 atcacatcga aatcagcgcc aaccagtacg ctaactgggg ttcgaaaggc gcatcggttg
     3541 atcgtctcga cgtggtggta ctgagcgcgc tggaaattga cacccagttc aacgttaacg
     3601 tgctgaccgg ctctgacggc gtactgcgtg gtgcttccgg tggtcactgc gataccgcaa
     3661 ttgcctctgc gctttccatc atcgtcgcgc cgctggtacg cggtcgtatt ccgactctgg
     3721 tggataacgt gctgacctgc atcaccccgg gctccagtgt cgatattctg gtcacagacc
     3781 acggtatcgc agttaacccg gcacgtccgg aactggcaga acgtctgcag gaagcgggca
     3841 ttaaagtggt ttccattgag tggctgcgcg aacgtgcgcg tctgctgacc ggtgaaccac
     3901 agccgattga attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga
     3961 tcgatgttgt gcatcaggtg aaggaataag ccatgcacct gcttcctgaa ctcgccagcc
     4021 accatgcggt atcaattccc gagctgctcg tcagccggga tgaaaggcaa gcacggcaac
     4081 acgtctggct caagcgccat cctgttccac tggtctcctt taccgtggtt gcgcctgggc
     4141 cgattaaaga ctgcgaggtc acacgccgaa tttttaatca tggcgtgaca gccttgcgtg
     4201 ccttagccgc aaaacagggc tggcaaattc aggagcaggc tgcactggtt tccgccagcg
     4261 ggccggaggg catgttgagc attgccgccc cggctcgcga cctcaagctc gccaccattg
     4321 agcttgaaca tagtcatcct ctcgggcggt tatgggatat cgatgtcctg acgcccgaag
     4381 gcgaaattct ctcccgccgc gactattcac tgccgcctcg ccgctgcctg ttgtgcgagc
     4441 aaagcgcagc cgtctgcgcg cgtggaaaaa cccatcaact gaccgattta ctcaaccgca
     4501 tggaggcact gctgaacgat gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg
     4561 acgtcattaa tcgatgagta cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat
     4621 ctgtcaccga aaccaggcct cgtggatcgc attaactgcg gtgcgcacaa agatatggcg
     4681 ctggaagatt tccaccgcag cgcgctggcg attcagggct ggttaccccg tttcattgaa
     4741 tttggtgcct gtagtgcaga aatggcaccg gaagcggtac tccacggatt acgcccaatt
     4801 ggtatggctt gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac gcataaaggc
     4861 agcatttttt ctttagggct gctatgtgcg gcaattggcc gtttgcttca gctcaaccag
     4921 ccggttacgc caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg cctgaccgat
     4981 cgcgaactgc gtaccaataa ttcacaactg acggcaggtc aacggttgta ccagcagctt
     5041 ggccttaccg gcgcacgcgg tgaagccgaa gcgggttatc cactggtgat caaccacgcc
     5101 ctgccgcatt acctcactct gctggatcag gggttagatc ctgaactggc attgctcgat
     5161 accttgctcc tactgatggc gatcaacggc gataccaacg ttgcatcgcg cggtggcgag
     5221 gggggcctgc gctggctaca gcgcgaggcg caaacattat tgcaaaaagg gggcattcga
     5281 acccccgccg atctcgatta tctccggcag ttcgacaggg agtgtatcga acgaaatctc
     5341 agtccaggcg gcagtgccga cctgctgatc cttacctggt ttttagcaca gatttaatta
     5401 tttaagcact tgataaattt ggaaatatta attttcggag aacccgtatg tctttagcaa
     5461 aagataatat atggaaacta ttggccccac tggtggtgat gggtgtcatg tttcttatcc
     5521 ctgtccccga cggtatgcca ccacaggcat ggcattactt cgctgtgttt gtggcaatga
     5581 ttgtcggcat gatcctcgag ccaattccgg caacagcgat cagttttatt gcggttacta
     5641 tttgcgttat tggcagtaat tacctgctct ttgatgccaa agaattagct gacccagcgt
     5701 ttaatgcgca aaaacaggcg ctgaaatggg gtctggctgg tttttccagc accacggtat
     5761 ggctggtatt tggcgcattt atttttgcat tagggtatga agtttccggg ttaggtcgtc
     5821 gcattgccct tttcctggtg aaattcatgg gcaaacgcac gctgacgttg ggttatgcga
     5881 ttgtcattat cgacattctg ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg
     5941 gtacggtttt tccggtcatt aaaaacctgc cgccgctgtt taaatcattc cccaacgatc
     6001 cgtccgcgcg tcgtattggc ggctatttga tgtggatgat ggtcattagt accagtctga
     6061 gttcgtccat gtttgtcacc ggtgcggcac caaacgtgct gggtctggag ttcgtcagca
     6121 aaattgccgg tatccagatt agctggttgc agtggttcct ctgcttcctg ccggttgggg
     6181 ttatcttgct tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac
     6241 acagtgaaga agtggcaacc tgggcgggtg atgaactaaa aaccatgggt gcgctgacac
     6301 gcagagagtg gacgctgatt ggccttgtat tgctcagctt aggtttgtgg gtatttggca
     6361 gtgaagtcat taatgctact gcggttggtc tgctggcagt ttcgctaatg ctggctctgc
     6421 acgttgtgcc gtggaaagac attacccgct ataacagcgc atggaacacg ctggtcaacc
     6481 tggcaactct ggttgtgatg gctaacggcc tgactcgttc tggttttatt gactggttcg
     6541 ccggtaccat gagtacgcac ctggaaggat tctcaccaaa cgcaacggtg attgtactgg
     6601 ttctggtgtt ctactttgca cactacctgt ttgccagcct gtctgcgcac accgcaacca
     6661 tgctgccggt tattctggcc gtcggtaaag gtattccggg cgtaccaatg gaacaactgt
     6721 gtatcctgct ggtgctgtct atcggtatca tgggctgtct gacgccgtat gcaaccggtc
     6781 ctggggtgat tatttacggc tgtggctatg tgaaatcaaa agattactgg cgtcttggcg
     6841 caatcttcgg ggtgatttac atctctatgt tgctgttggt tggctggccg attctcgcca
     6901 tgtggaacta atacgttcgc caaacgctga ataaatcatc acgcccgcca ggtaagccca
     6961 cctggcgggc ttttttatga tttaatagat agtattcagt gtatgtatga gttccacacc
     7021 cattatgaaa gcattctggc gtaacgccgc gttgctcgcg gtttctctgc ttcccttctc
     7081 ttctgccaac gccttagcgt tgcaggcaaa acagtatggc gattttgatc gctatgtcct
     7141 ggccctctcc tggcaaaccg gattttgcca gagtcaacac gatcgaaatc gtaacgaacg
     7201 agatgaatgt cgcctgcaaa ccgaaacgac caacaaagct gattttctga ccgtacatgg
     7261 tctgtggcca ggattgccta aatcggttgc tgcccgtggt gttgatgaac gccgctggat
     7321 gcgcttcggt tgcgctactc gcccaatccc gaatctacca gaagcgcgcg ccagccgaat
     7381 gtgttcatcg ccggaaaccg gattatcact ggaaacggcc gctaaactaa gtgaagtcat
     7441 gccaggagct ggcggacgtt cctgcctgga acgctacgaa tatgccaaac acggtgcctg
     7501 ctttggtttt gatccggacg catacttcgg tacgatggta cgcctgaatc aagaaattaa
     7561 agagagcgaa gccggaaaat tccttgcgga taattacggt aaaacagtga gccgccgtga
     7621 ctttgacgcc gcctttgcca aaagctgggg aaaagagaac gtgaaagcag ttaagctaac
     7681 gtgccagggt aaccctgcgt atttgactga aattcagatc tcgatcaaag ctgacgccat
     7741 caacgctccg ctttctgcaa actcattctt gccacaacct cacccaggta actgtggcaa
     7801 aacctttgtg attgataaag cgggttatta atcatctgat ttacaaaaca cgctaattcg
     7861 tcttccataa accaaggcga ttaagattta tacataatgc gctgcattga ggaattccct
     7921 gaatgtgacg caaatcactt caaagagtga gcaacagtca gtatcatcat gtgagttaaa
     7981 ccctcgccgc ctgacggtga gggttttctt ttgggtatgt ttatctgcga cactcgcagc
     8041 accgacgaca tggagtaaaa atgtccagac caactatcat cattaacgac ctggatgccg
     8101 aacgcatcga tattctgctg gagcaacccg cctatgctgg tttgccaatc gccgacgcgt
     8161 taaacgcaga gttggatcgc gcccaaatgt gttcgccaga agagatgcca cacgacgtgg
     8221 tgacaatgaa cagccgggtt aaattccgca atcttagcga tggcgaagtg cgtgtgcgca
     8281 cgctggtgta tccggcaaaa atgaccgata gcaatactca gctttccgtt atggctccgg
     8341 taggtgccgc actgctgggg ctgcgcgttg gcgattccat tcactgggaa cttccgggcg
     8401 gcgttgcaac ccaccttgaa gtgctggaac tcgaatacca gccagaagct gctggcgact
     8461 acctgcttta atcccatctg cacaagcgat aactcgcttt gcccagagga tgcactcgca
     8521 tcctctttac gcaaaatcag caatttttcc tggttctcac tgttcacttt tgctgcaaaa
     8581 ttattccgtt tcgtttgtgg gaataaccat ggcagaagca ttttacatcc tgataggatt
     8641 tctgattatg gcggcgatta tcgtcatggc tgtcctttac ctcgaaaacc actcctgacg
     8701 atcatattgt tccccccggc atcgcattca ccagacctga aaccgccttc tgcgccgcct
     8761 ctgcgctttg tgcaccgggt accagaatca ccttacggca ctcctcttca cgtttttcga
     8821 aaatctcata tccccgggcg gcctcttcaa acggcatata gtgggtaaca atttcttctg
     8881 gtttcagtaa tcctttctca attaacggta ataattctcc cagccatgcg tgaacgtggg
     8941 tctgtcccat tttaaacgtc aaccctttat caaaggcgtc gccaaacagg aaaccgtgaa
     9001 taaatccagc gtagacgccg ggtacgctaa caatgccgcc acgcctgact gccgcaatac
     9061 actggcgcaa cgctttaccg ctgctgccct ccagtttcag gttagtcagc accgtttccg
     9121 tggtgctgcc tttcgcttca aaaccgacgg cgtctattac tgcatccacg ccccggtgac
     9181 ctgccgtttg ttcaataatt gactgtgccg gatcgctgtc ttcatcaaaa ttaatcggga
     9241 tcgcgccgta gcggtcggcg gcgaaatgca agcggtaggg atgatgatca acaacaaaaa
     9301 tctgttccgc accgagcaac cgtgcacagg cgattgtcaa caatcccaca ggaccagcac
     9361 catagactgc aacgcttgaa ccttgttgga tctgcgcatt ttttgctgcc tgccatgccg
     9421 ttggcagaat atcagaaagg aaaagggctt tatcatctga aagcaaaggc ggtactttaa
     9481 acggccccac attcccttta gggacgcgga catattccgc ctgcccacca ggaacgccac
     9541 catacaggtg actataacca aacaatgccg cgggagctgg tatctgtttt ttattgagcg
     9601 cagcgccttt acccgcattg gtattttcgc aggcggcata ttgctgcatt cgacagaaaa
     9661 aacagtcgcc acaagcaatg acaaacggaa ttaccacccg gtcgcctttt tgcaaatttt
     9721 ttacgtcctt tccggtttca acgacttccc ccataaattc atgaccaaaa atatcgccat
     9781 gtttaacctg gggtattttg cctcgataaa gatggaggtc agagccacag atcgccgttg
     9841 ccgtaatacg cagaataata tcatctgcct gttcaacgcc cggatcggga acattttcta
     9901 cctgaacgtg atgtgggccg tgataagtca atgctttcat gcaacctcca gagacagttc
     9961 aaaaaagagt cagtctttaa gggtagctat caccgcggga aaacgggcgg taccacaggt
    10021 tttgggagaa ttcctggtgt tcagtgagaa tatattcatc acagaaaccg ttcgtaagga
    10081 aaaagaacca gacaaatatc agggataaat attataatca tggcgaatga tttgttcatg
    10141 attaacaggg agaaagggtt atgtataaga caatcattat gccagttgat gtatttgaaa
    10201 tggaattgag cgacaaagct gttcgccacg ctgaattcct cgctcaggat gacggagtta
    10261 ttcatctact tcacgtacta cccgggtcag ccagcctgag cctgcaccgt tttgccgctg
    10321 atgtgcgtcg ttttgaagag catctgcaac atgaagcaga agaacgtctg caaacgatgg
    10381 tcagccactt caccatcgat ccttcccgca ttaaacaaca tgtccgtttt ggtagcgtgc
    10441 gggatgaagt caatgagttg gcagaagaac tgggggctga tgttgtagtt atcggttctc
    10501 gcaacccatc gatttcgacc catctgttag gttctaacgc ctcgagcgtg atccgccacg
    10561 ccaatctgcc ggtgctggtt gtgcgttaat tgccagaata agtatcccgc cctgcccgga
    10621 gccatcccgg cggggctttt taatgccata aaaaagccgc caggtttgac cccggcggct
    10681 aagcaattgc aggtgaatct tacttcttct tatacagttt tggtgcgaat caggtagtca
    10741 aaagcactca gagaggcttt ggcaccttcg ccagtggcga tgatgatctg cttgtacgga
    10801 accgtcgtac agtcacccgc tgcgaacacg cctttaacgt tggtttcgca tttcgcatca
    10861 atgataatct cgcccatgcg gttacgttcg actgcgcctt ccagccagtt ggtgttcggc
    10921 agcagaccaa tctggacgaa aataccggcc agttcgatgt tgtgaatatc gccgctgaca
    10981 cgatctcgat attccagacc aacgactttg ctgccgtcgc ctttcacttc cgtagtttgc
    11041 gcattcagaa taatgtcgac gtttttcagg ctgcgcagtt tgtcctgcag cacctggtcg
    11101 gctttcattt ctggcgcaaa ttcgagcagc gttacgtgct caacgatacc cgccaggtcg
    11161 attgccgctt ccacgccgga gttaccgccg ccgataaccg ctacgcgttt gcctttaaac
    11221 agcgggccgt cgcagtgcgg gcagtaggtc acgcctttgg tacgatactg atcttcgcca
    11281 ggaacgttca tgttgcgcca ttttgcaccg gtcgccacga taatgctgcg tgctttcagt
    11341 accgcgccag aagctgtttc aatctgatgc agaccacctt caaccgctgc cgggatcagt
    11401 ttgctggcgc tctggctgtc gatcacatca acgtcgtatt catcaacgtg aactttcagc
    11461 gcgcctgcca gcttctgccc ttcagtcttc ggtacagaaa tgtagttttc gatatcaaca
    11521 gtatcgagga tctgaccacc aaaacgttcg cccatcagac cggtacggat gcctttacgt
    11581 gcggagtaaa ttgctgccgc tgcacccgcc gggccggaac cgacgattaa tacgtcataa
    11641 gcatcacgct tgttcagctc ttctgccgca cgtttttccg cgccagtatc aattttggcg
    11701 acgatttcag tcaacgtcat gcggccctga ccaaactctt tcccgtttac gaacactgcc
    11761 ggaacgccca tcacgttgcg atcggtgatt tcgttctgga aggtgccgcc gtcaattgca
    11821 gtgtgcttga tgcgcgggtt cagtacgctc atcaggttca gcgcctgcac cacgtccggg
    11881 cagttatggc aagagagcga gtaataggtt tcgaattcaa aatcaccgtc aatatggcga
    11941 atctgctcca gcagagactg cgcttctttc gacggatgac caccggtcca cagtaacgcc
    12001 agtaccagcg aggtgaactc gtggcccagc ggggagcctg caaaacgtgg cccctggttg
    12061 gaacctgggt tggtgatcag gaaagacggc ttacgcaccg gcaagctgtt atcttcttta
    12121 aaggtgactt tgtctgacag ttctgcgatt tcagccaaca gttccttgat ttctgccgat
    12181 ttagcgctgt catccagcgt ggcaattaac tcaacaggct tggtcaattt ctcaaggtaa
    12241 gccttgagtt gagttttcat atttgtgtcg agcatgttta tctcctgggc tttaaacatc
    12301 atcatgcaag cggcctctgc ggctacctgg atgcagcttg catcatggtg cggggaagaa
    12361 aatcgggtgc cgcagcaccc gaagaattag gtgaatttcc gaagtatttc gcgtcagagc
    12421 aaggcggcta gcctgtgaat ccccgggagc ttacacaagt aagtgaccgg ggtgagcggg
    12481 cgacgccaac gccgctatgg cgtgaaagac gaaggaaatt tagattttac caaccaggtc
    12541 cagagacgga gccagagttg cttcaccttc tttccattta gccgggcaaa cttcacctgg
    12601 gtgagaagct acgtactgtg ctgctttgat tttacgcagc aggtcagacg cgtcacggcc
    12661 aatgccttca gcggtaactt cgattgcctg gatgataccc tgcgggtcaa caacgaaggt
    12721 cgcacggtca gccagacctt catcttcacg catgttgtcg aagttacggg tcagggcgcc
    12781 agtcgggtcg ccgatcatcg catatttgat tttagcgatg gtttcagagc tgctgtgcca
    12841 tgctttgtgg gtgaagtgag tatcggtaga tactgcgtat acgtctacgc ccagtttctg
    12901 cagttcttcg tagtggtcag caacgtcacc cagttcggtc gggcatacga aagtaaagtc
    12961 agccgggtag aagaagaaga cgctccagcg gccttcggta tctttttcgg tgatttcgat
    13021 gaattcgccg tttttgaatg cctggttttt aaaaggttta attttggtgt taatcaagga
    13081 catctatact tcctccgtgt tttcgatgag atgtaaggta accaattttt gccgattcgg
    13141 ctaatgcgtt tccattatca aatcgataag ttttacctta caaccttcgt aagacaactt
    13201 tgtgaacttt gcaccgccag cggcgataaa aagtaaaatg gccccctttc caggtgaccg
    13261 tgtaatctga actacccttt ggtatcttca gtgcctgcct gcaggctgag gtgttgccct
    13321 tacaaatgca acaacgacat ggattacaac accctcataa acaaagggca atcacctgat
    13381 ctaagctctt acctatgaca gtgataggtt atgcctttta ctcgactttt gcactgactg
    13441 aaaaggacaa attaatgtta aaaaagatac ttttactggc tctgcttcct gcaatcgcct
    13501 tcgcagagga acttcctgct ccagtaaaag cgattgaaaa acagggcatt acaatcatca
    13561 aaacattcga tgcccccgga ggaatgaaag gttatctcgg aaagtatcag gatatgggcg
    13621 tcaccatcta cctgactcca gatggtaagc acgctatctc tggttacatg tacaacgaga
    13681 aaggtgaaaa cctgagtaac acacttatcg aaaaagaaat ttacgcacca gccggacgcg
    13741 aaatgtggca acggatggaa caatcccact ggctcctcga cggtaaaaaa gatgcgccgg
    13801 tcattgtcta cgtcttcgcc gatccgttct gcccatattg taaacagttc tggcagcagg
    13861 cgcgcccgtg ggtagattct ggcaaagtgc aattaagaac attgttggtt ggggttatca
    13921 agccagaaag cccggcgaca gcagcggcaa ttcttgcctc caaagatccc gcaaaaacct
    13981 ggcaacaata tgaagcctct ggtggcaagc ttaagctaaa cgtgcctgca aacgtaagta
    14041 cagagcaaat gaaagtgtta agcgataacg agaaactcat ggacgatctg ggggcaaatg
    14101 tcacaccggc tatctattac atgagtaagg aaaatacgct acaacaggcc gtggggttgc
    14161 ccgatcagaa aacgcttaat atcattatgg ggaataaata aaaggtaatt catggatggt
    14221 tgaactatat ccctgctttt caaccatcct aaaaaagcat gaattgctcg ctctgcccta
    14281 agtggctgtg ctattttaga aaaacttaat cctgagttaa gcaaaaaaac ataatcaata
    14341 aaaatatata cgcaaaaaac attgattaag tgaatatatc atggaagaaa aatataaccg
    14401 gagtagtgta tggccaatct ctacgacttg aaaaagttcg acttaaatct tcttgtcata
    14461 tttgagtgta tttaccagca tttaagcatt agcaaagctg ctgaatcact atatatcacc
    14521 ccatcagccg tgagccagtc gttacaacgc ttacgtgcgc aatttaatga ccctttattc
    14581 attcgctcag gaaaaggtat cgcgccgacg acaacagggt taaatctgca tcaccatctt
    14641 gagaaaaatc tcagaggcct tgaacaaaca attaatatag tcaataagtc cgaactaaag
    14701 aaaaacttta ttatctacgg ccctcagctc atttcttgct ctaataatag tatgctgatc
    14761 cgctgcctac ggcaagattc ctctgtcgag attgagtgcc atgatatcct catgtcagca
    14821 gagaatgctg aagaactgtt ggttcaccgc aaagccgatc tggtgatcac ccaaatgcca
    14881 gtgatcagcc gttccgtcat ttgcatgcca ttgcatacga ttcgcaatac tctaatttgt
    14941 agtaataggc atccacgaat tactgacaat agcacttatg aacaaattat ggcagaagag
    15001 ttcacccaat taatatctaa atcagcaggg gttgatgata ttcaaatgga aatagacgag
    15061 agatttatga atcgcaaaat ctcatttcgt ggttcctctt tgctgacaat cattaatagc
    15121 attgcagtta cagatttact gggaatagtc ccctatgaac tttataattc ctaccgtgac
    15181 tttcttaact taaaagagat aaaactagaa catccgttac cttcaataaa gctttacatt
    15241 tcctataata aatcttcttt aaataatttg gtgttctccc gatttattga ccgtctaaat
    15301 gagagttttt gatatataac tacagacagc tactataact ttcatctatt tattcacagc
    15361 gcgaatgaaa aataaaacac tctgctttac agtgtttact ttataattaa cagacttatc
    15421 tcactgatca ccctgtaacg ttcagagagc gtcttcagat gtctatttat aaaattcctc
    15481 ttccgctcaa tattctggaa gctgcacgag agcgtattac ctggacactc aacaccctac
    15541 cccgcgtttg tgtctcattt tctggcggca aagactctgg tttaatgctg catctgacag
    15601 ccgaacttgc ccgacaaatg ggcaaaaaaa tctgcgtttt gtttatcgac tgggaggcgc
    15661 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
    15721 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
    15781 ccgaatggca gtgctgggaa cctgatgtcg aatgggtacg tcaacctccc caagatgcga
    15841 taaccgaccc tgactttttc tccttttacc agccaggcat gaccttcgaa caatttgtac
    15901 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
    15961 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
    16021 acgataaacc ctggactacg gccgcaccag acggtcatag ctggtacatt taccccattt
    16081 acgactggaa agtggctgat atctggacct ggtatgcaaa tcatcaaagt ctctgcaacc
    16141 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
    16201 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
    16261 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
    16321 acaatcattt ctatgggcat cggaaaatcc tcaagccaga acatttagac tggcaagaat
    16381 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
    16441 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
    16501 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
    16561 acgattactg gtgtcgggca ttatcattta gccccacaaa atcgaagaac tatcagcgtt
    16621 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
    16681 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
    16741 tgaaatccgc atggcgatcc atcaggttag cccctttcgt gaagagccag tggattgcgt
    16801 tctgtgggtt aaaaacagcc aacttatgcc taatgattac aacccaaaca acgtggcacc
    16861 acccgaaaaa aaactgctgc aaaaatccat tgaaatagat ggttttaccc aacccattgt
    16921 ggtcacacat acagataaaa atgccatgga aatcgtggat ggttttcacc gccatgaaat
    16981 tggtaaaggc tcatcatcat taaaattacg cttaaaaggt tatttgccgg tgacctgtct
    17041 ggaaggtacg cgtaatcaac gcatcgccgc gactattcgt cataatcgcg ctcgtggacg
    17101 ccatcaaatc accgccatgt cggaaatcgt ccgtgaactt agccagttgg gatgggacga
    17161 taataaaatt ggcaaagagc tgggaatgga cagcgacgaa gtgctgcgcc tgaagcaaat
    17221 taacggcctg caagagttgt ttgccgaccg tcaatattcc cgcgcctgga cggttaaata
    17281 gctaaagctg gcgcaggcgt tcagctgctg ccagcaacgt cgattccttc ttggcaaaac
    17341 agagacgaat cagtttatgt gggaagggat cggcgcaaaa caccgacagc ggaatcgccg
    17401 ctacgccgtg ctcctgcgtc agccactggc aaaactcaac atcatccagg gtagaaaccg
    17461 cgctgtaatc caccagcaaa aagtatgtac cttcacacgg taaaatctcc agccggcttt
    17521 catttaaagc attcaccaga atatcgcgct tctggcgata aaagtccggt aacgcaagat
    17581 aatgctcagg ttctgcacgt agcatatcag caagcgccag ctgtgccggg gtattcaccg
    17641 aaaaggtcag atactgatgt accttgcgaa tttcggcgct gatgggcgct ggcgcaacac
    17701 aataacccac tttccagccg gtcatatgat aggtcttgcc aaatgaagaa accgccactg
    17761 cccgctcacg cagctgcgga tgcgccagca cactggcatg gccctgttgt gaaaagttga
    17821 tgtgctcgta gacttcatcg ctaatgacaa aaatctcgtg cccggcgatc gcctgccaca
    17881 aagcggcgaa atcagcctgc tgccagacag ttgcactggg gttatgcgga gtgttgagga
    17941 tcaccagtct ggtgcgctcg cttaataatg cggcaaattc ctgccagtca acgcgaaaat
    18001 gcggtggttg cagtgccata cgcttcacta ttcccccaga aagcgcgatg gcgggggcgt
    18061 aactgtcata gctgggatca aaacaaatca cttcatcgcc attgcgcacc agtgcggtaa
    18121 tcgccgcgta taacgcttcc gtcgcccctg ccgttacggt gatatcgcta tcggcatctg
    18181 gttgatagcc atacaaacgt tccgttttct gagcaatcgc ctcgcgcaag gcctgcacgc
    18241 cggtcatggg cgcgtattgg tttgccccct gtgcaacgtg gtgcgccagc cgctcctgta
    18301 aatagcgcgg accatcaaaa tcaggaaagc cttgcgacag gttaatcgcc tggtgttgct
    18361 gcgccagcgc gctcatctgg gtgaaaatag tggtgccaag ttgtggaagt ttgctttgtg
    18421 gaatcagagg gttatttgtc atttttattg tacctgacga agttgcgtgt tgaaggcact
    18481 ataacacgat gctagtattt ggcaatcaag acgtttagat gtctaaataa aacaataagg
    18541 acaacacaac atgcctcaca atcctatccg cgtggtcgtc ggcccggcta actacttttc
    18601 acatccagga agtttcaatc acctgcacga ttttttcact gatgaacaac tttctcgcgc
    18661 ggtgtggatc tacggcaaac gcgccattgc tgcggcgcaa accaaacttc cgccagcgtt
    18721 tggactgcca ggggcaaagc atattttgtt tcgcggtcat tgcagcgaaa gcaatgtaca
    18781 acaactggcg gctgagtccg gtgacgaccg cagcgtggtg attggcgtcg gtggcggtgc
    18841 actgctcgac accgcgaaag ccctcgcccg ccgtctcggt ctgccgtttg ttgccgttcc
    18901 gacgatcgcc gccacctgcg ccgcctggac accgctctcc gtctggtata atgatgccgg
    18961 acaggcgctg cattatgaga ttttcgacga cgccaatttt atggtgctgg tggaaccgga
    19021 gattatcctc aatgcaccgc aacaatatct gctggcgggg atcggtgaca cgctggcgaa
    19081 atggtatgaa gcggtggtgc tggctccgca accagaaacg ttgccgctaa ccgtgcgact
    19141 ggggatcaat aatgcgcaag ccattcgcga cgtcttgtta aacagtagcg aacaggcgct
    19201 gagcgatcag caaaatcaac agttaacgca atcattttgc gatgtggtgg atgctattat
    19261 tgctggtggt gggatggttg gtggtctggg cgatcgtttt acgcgtgtgg cggcagctca
    19321 tgccgtgcat aacggtctga ccgtgctgcc gcaaaccgag aagtttctcc acggcaccaa
    19381 agtcgcctac ggaattctgg tgcaaagcgc cttgctgggt caggatgatg tgctggcgca
    19441 attaactgga gcgtatcagc gttttcatct gccgactaca ctggcggagc tggaagtgga
    19501 tatcaataat caggcggaga tcgacaaagt gattgcccac accctgcgtc cggtggagtc
    19561 cattcattac ctgccagtca cgctgacacc agatacgttg cgtgcagcgt tcaaaaaagt
    19621 ggaatcgttt aaagcctgac gtacaaattt agcagcagcg cgcgccgcct tttccaccgt
    19681 agcgcgcgtc ctgccgctca cggaaaaact cttcgtaggt catcggcgtt tgatcgggat
    19741 ggttaacccg catatgttcg acatagttgt cgtaatcagg cataccaatc atcagcttcg
    19801 ccgcctgacc taaatatttt ccggcttttg ccagtgaatc aaacatcttg gcctccgcta
    19861 accatgttgc tgaaagagtg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatgct
    19921 atcaacgcgc tcggtcagtc ccctcgcccc accggggaga gggttagggt gagagggaac
    19981 cctgggcact aagccaacat tcgccaacat cccccctcac tctgacttta gtgtgcgcct
    20041 tttgcctgcg ccacgatctc ctcaacattt tccggcatcg gctcatacgg cgtttctttc
    20101 gccgttggct tcggatcttt caatgccgca agtgccgtct taatcgagaa cagtgccaga
    20161 accaccacga ccaccataaa gaagatggtt aacccggcat ccagacggtt attgaacacc
    20221 agttgcgcca gctgtgactc agtatactgc gacggaatat tgccgctgtc gatcattgcc
    20281 tggaacttat tagcaatggc caggaagccg actttcgcat ccgggctaaa cgctttctgc
    20341 cagccagcgg tcagggtaca aatcagcagc caggccgttg gtaccagcgc cacccaggcg
    20401 taacgttgac gtttcatttt gaacagcacc acggcacaga gcatcagcgc catccctgcc
    20461 agcatctggt tggcaatacc aaacagcggc cacagagtgt taataccgcc cagcggatcg
    20521 accacaccct gatggaggaa gtagccccac gccagtacgc acagcgccgt tgccagcagg
    20581 ttagcaggca gtgaatcggt ccgcttcagg ccaggagaca ccacgcccag cagatcctgc
    20641 aacataaagc gcgcagcacg cgtacctgca tccaccgccg tcagaataaa cagtgcttca
    20701 aacaaaatgg cgaagtgata ccagaacgcc acatccatca tgccgcccag cgcgccgtgc
    20761 agaatgtagg ccatccccac cgccagcgtc ggcgcaccgc ccgcacggga aatgatcgac
    20821 tgttcaccca cttcgctggc aatctggttt agcgtatctg gtgtaatgct aaagccccag
    20881 ctactcacca cctgcgcggc agaagcgacc acatccgccg tccctgccgg agccagcacc
    20941 gccatcgggc tgttcatggc aaaatacacg cccggatcga tgatacaggc ggaaaccagc
    21001 gccataatcg ccacgaagga ttcaattaac atcccaccgt agccgataaa gcacgcctgc
    21061 ccttcgttcg ccagcatctt cggcgtggtc ccagaagaga tcagcgcatg gaagccagac
    21121 accgcgccac aggcgatggt gataaacagg aacgggaaca ggttaccggt ccataccggg
    21181 ccagtgccat cgacaaattt ggtcagcgca ggcatggtca gcgtcgggcg cataatcaaa
    21241 atgcctaccg ccagaccgac gatagtcccg attttcagga aggtagagag gtagtcacgc
    21301 ggggccagca gtaaccacac cggcaacacc gccgccacaa aaccgtaacc caccagcatc
    21361 caggtcaact gcacgccggt aaagtcaaag tacggtgccc aggtcggact ttctgccacc
    21421 cagccgccag agataatggc gaaaatcagg aataccaggc caatcaccga cacttcgcca
    21481 atacgccccg gacgcagata gcgcaggtag atccccataa acagcgccag cggaatggtg
    21541 aacgcaacgg tatatgttcc ccacgggcta tgagtcaggg ctttcaccac gatcatcgcc
    21601 agcacggcaa ggataatgac catgatcata aagcaggcca ccagcgcaat caccccggcg
    21661 gttggcccca tctcttcttt gaccagctca cccagcgagc gaccgtcacg acgcgtagaa
    21721 acaaacagca ccatgaaatc ctgcaccgca ccggcgagaa ccaccccagc gagcagccag
    21781 atcatccctg gcaggtagcc catttgcgcc gccagtaccg gccccaccag cggacctgct
    21841 ccggcaatgg ccgcaaaatg gtgaccgaac agcactttct tgtccgtcgg cacatagtcc
    21901 agcccgtcgt tatggcgcac cgctggcgtc atacgcgtcg ggtcaaccgc cagcacattt
    21961 ttggcgatat acagcccata aaaacggtaa gcgatcagat agatacagac cgacgccacc
    22021 acaatccaca gcgcgttgat ctgttcccca cgatttaaag caatgtatcc cagagcaaat
    22081 gctcccatta cagagagcac tgtccagacg aggtatttcc ctgatttgtt catagttgtt
    22141 atccgtgtgc gtgtccatag agatgttaca ttttgtttct ataacatctt tgtacaattt
    22201 aacaacacag caaccatgta aatcggagtt gaaaccaata tttaacttaa caatgttaac
    22261 tcgatcactc cgttaaccga gttttcctgc aatctcatct aattctgtcg ggttcatccc
    22321 aaaactgccg tacccagccg acaagtgcag caacgccgcc cctgttcatc gaaaacgacg
    22381 atttcccagc tctgattttg ccgaccaaga tgcagcggct ggcagacgcc gcgtaccttt
    22441 ccctcagaca ccgggcgatg gtgtgttgca ttaagttctg tgcctaccac acactgtccg
    22501 tcgcgggtca tcataaatcc ggccatcgat cccagcgttt ccgccagcgc cgccgacgcg
    22561 ccgccatgta gtaaaccgaa cggctgatga gtacgggtat caaccggcat ttcggcttcc
    22621 agcacatcat cgcccagacg ggtatacaca attcccagat gcgccaccat tgtgttatcg
    22681 ctggtggcgt tcagttcgtc gagcgttaaa tggcgtttcc agatcatgct tatgccccca
    22741 gcgttgagcc gccatcgacc acaatatcct gtagggtgat atggctggcg aggtcagagg
    22801 cgaggaacaa aatcgtgttg gcgatctctt gtggacgggc gattttcccc agcggaatgc
    22861 cgagtttaaa ctgctcgcca aagccgcgaa tacgctgttc ttcggcgtca tcgctcaccc
    22921 acagcgtgcg ttgcatatcg gtgtcggtgg agcccggcga aaccacatta cagcgcacgc
    22981 cgctacccgc cagttccagc ccgacgctca acgccaggct tttcagcgcc gctttcgatg
    23041 cgccataagc actcatgcca atacgcggcg tgtgcgcggc gtcggacgcc acagtgacaa
    23101 tcgccccgcc ccgctgacgg cgaaactggt tcatggtttg ctggaacagg ttaaacgcac
    23161 cgccgacgtt aaccgcaaaa gtctgctgcc agtcctcttt actgagctga tcggtcgcgc
    23221 ccatgcgcaa aattcccgcc gcattgacca gcacgtccag tcgctccgtt tcagctaaca
    23281 gtctctgaca tacctgcgcc acctgcgcag catcggcaac atccatcact tcggtcgcaa
    23341 agggatattg ctcctgagtg aacgcttgat caaaacctgt aactttcgct cccgcttcaa
    23401 caaacgccag cgccgtggcg tagccgatac ctttccccgc gccagttacc cagacatttt
    23461 taccgctgaa atccattatt tcacctcgcg ggagagtagc ttccaccagg cgtcgatggt
    23521 cgggtttttc gccagcatga caaagtcgat atcgccatgc actttgcgcc agcgcgccgc
    23581 cagcgccatc atgcgcaccg aatccagacc gtagtcgatc aggttgtcgt catcgaacgg
    23641 ttcatcggac tcgtccagca acggcaggat cacctcacgc agcgccgctt tgctggcggg
    23701 gataggtgct ggcagtaatt cttcggtcat caccacccgg ccagaacgtc cggccacata
    23761 tttcagcgac atcaaatgct cgtcacggct gaaatcggcc agcgcatccg ccaccataaa
    23821 cggtttaata tcgcgcataa atgcgtcggt tgcggtggtc atacagccaa tgtgggcata
    23881 taccccggta ataatcagct ggttacgtcc actctctttc agcatttgct ccagcggaga
    23941 acgatgaaac gcgctgtagc gccacttcac cagcaccgtg tcgtcggcat ctggcgtcag
    24001 gcgatccacc accttttgct gttccggcga gcgggtcagg cccggccccc acatatcatt
    24061 caacagcgcc cgatcttcat cgctctgctc tttcggctgg gcggtgtaat aaaccgggat
    24121 attgtgctgt ttgcagtagt cgcgcagcgc agcaatattc gcgatcacct gctccatcat
    24181 cgggcagttc tcgccccaga agctgacaaa atagtcctgc atatcatgga ttaacaacgc
    24241 ggcacgttgc ggttcaaagg cccagtcaac tttattctgc ggaatatcgt gagactccgg
    24301 cagtgcgtaa gcctgtaatt ttggaatagc catcgtgttc tctccttcag gctgatgcgc
    24361 gtgacgccag ccactgacgt aattgttttt tatcgacttt cccgaccgcc gtcagcggaa
    24421 gtgaatccac acactccacg cgatccggta atttaaattc ggcaataccc tgttcacgca
    24481 ggaaacgacg cacctgcacc gcgcgcagcg gctcttttac caccagataa gcgcagcttt
    24541 tttcgcccat cagctcatct tccatgctca ccagtgcggc gtagatcacc gccgggtggc
    24601 gcagcagcag gttttcgatc tcttcggcag cgatcttctc gccgccacgg ttaatctgat
    24661 ctttctcgcg cccctgcacg gtgatgtaac cctctggatc aatagagatc agatcgccgg
    24721 aacagtaaaa accgttggca tcaaaggcgc tggcattgtg ctgtggactt ttgtaatagc
    24781 cgcggaaggt gtacggcccg cgcgtcatca ggcgtccgac ttccccttgc ggcagtggat
    24841 ttccttcggc atcggcaacc catacttcgt catccggaca cattgggtaa ccctgggtat
    24901 ggataatttt ctccgcgcta tcatcaagtc gggtgtagtt caccagccct tccgccatgc
    24961 caaacacctg ctgcaactga cagccaatct cagcgggaat acgcgccgca agggtggcag
    25021 aaagacgtgc gccgccgacc tgtaacagtt tcagcgaggc aagctgcgcc cggctttcgc
    25081 cttcgatcag cgcctgcaac cacaggctga ctgcgggtgg caccagcgcg gtaacgttaa
    25141 cctgatgttt ttcaatcaat gggaaacaga gcgtggcgct gggatcggcc gccagaacaa
    25201 ccgttcctcc ggcaagaaag acgcccagcg atcctggcga actcatggcg tagttatgag
    25261 ccgccgggat cgcgcacagg tagcgtgtct gttgtgtgaa ctgacaaatc tcgacgctac
    25321 gacgcacgct gtagtagtag tcgttatgag tgcgcgggat cagtttcggt gtgccggtgg
    25381 tgccaccgga aagctggaaa taggccactt catcagcagg tgatggcgtg gcagtaaaat
    25441 cctcagccgg atggttaatc gcatcctgca agttatgctc accgctgtcg ttgagcagtt
    25501 gcaccacgcg aatggaggaa tgttctgtga cgaaagtatt gaggaaatca tccccgctaa
    25561 acagcgcatg ttggcgatcg gcaatcagca atgcgggttc aatctggctg gcataggcgt
    25621 tcagttcact acgctgatgg ctgaacaacg ccagcaccgg cgcaacgccc agtttcagca
    25681 gcgcgaaaaa ggtaatatac aattcagcga cgttacccag ttgtaccagc gcggtttcac
    25741 caggtttaat gccctgacgg cgtaaactac acgcgaggtt atccgccgcc tgattcagct
    25801 cccgataact caactgtcgc tcgccgtcga taaccgcgat gctgtcactc gcagcatgtc
    25861 gcgtcagaat gtcggtcagc ggcaaatcct gccagtagcc tttttcccga tagcgacggg
    25921 caaactcttc cggccagcgg gtgaatggaa tgctcatcct cgctccttaa tgcaatccaa
    25981 aaacgttcaa catggtagaa agtttgacgc ctgtttcgcg ccactcaccc aacggtgacg
    26041 acgcaggcac aatccccgct ccggcaaaca gacgcacctg attttcccgc agcttcgcgc
    26101 agcggatggt caccacccat tcgccgttac cttcgctgtc acaccaaccc acaatgccgc
    26161 caaacagttc gcggtcgaac ggttccagtt cagcaataac ctgggtcgcg gcctgatgcg
    26221 ggaaaccgct cagcgcgggg gtcggatgca gcagacaggc cagagtcagt gcgttttctt
    26281 gcgaattcgc tttaccttca aagggagttg cgagatgcca cagcgtcggc gtggtgatca
    26341 gctgtggaga agaaggaacg tgtaactcac tactgcgttc gcgcagtacc tctttcatcg
    26401 cctgagtcac cagttcatgt tcatggcgat ctttttctga cgccagcaga cgattacctg
    26461 cttcgcgatc gagcacttca tccggctgac gacgcgcgga accggctaac ggaatggagc
    26521 taaaacgctc gccgtcttta cgtagcagca gttccgggct ggcccccagc aggacgccac
    26581 catcagccag cggaacatgg aagttgtaac taaccgggtt ttgcgcaatc aaccgttcca
    26641 gcaatacgcc actatcaatg gcggcgtcag tggtgatatc aatcaaccgt gacaacacca
    26701 ctttgtcgac ctgcggcgtg gcggtaagtg cggcggcgcg ggcaaccatc tgttcaaacg
    26761 tggtttgctc cggaattgcc tggcgttcca ccacattcag cgactggctg cgggtgaaac
    26821 ggcgtgcgga agcttgtttt tcctgacggg agaacgactg ccaggattca ggaatataca
    26881 gcgacgaagg ctgacgtgga tcgaagggaa tcgccccgac catcaccgga tttttgatgc
    26941 cctgcgcttt ggcatcggca aacagcgcgg cgagtttttg ctggaaggga ctgtcgggcg
    27001 aatccccgtt cacagccggt tcatcgaagc gggcgaaaca tcctgacgtc gtaaaactgc
    27061 ggtacggcga cataaagaaa aagcgattgg gcgcaagtgt tgccatggtc tgctgtactt
    27121 cctcagccag tgacgtatcc atatcatcct ccacaaaatg ataaaggctt taataatgat
    27181 tatcatttat attttcggtc gctaacctaa aagcaaacgc gcactatgtc aactcttgag
    27241 gtaacgcaat ctgcaactcg gggttgcatc tggttgtggt tacaatgaaa atgagaagca
    27301 ttattgatgg attcgcataa gcgcaatgtg atgtcctgcg ccgttctgcc ccctctccct
    27361 tccagggtga gggctggggt gagggttaat gttcgcacca gtgctggctg ttcccctcac
    27421 cctaaccctc tccccaaagg ggcgagggga cggattgtgc gctttgtcga atttgtcatt
    27481 acgcccttaa ccttattaat aacaggaagc tgatttgtga gactcgcccc gctctaccgc
    27541 aacgcccttc tattaacagg acttttgctt tcaggaatag ccgcagttca ggccgctgac
    27601 tggccgcgtc agattactga cagccgtggc acacatacac tggaaagcca gccgcagcgt
    27661 attgtttcca ccagcgtcac cctgaccggc tcactgctgg cgattgatgc accagtgatc
    27721 gccagcggcg cgaccacgcc aaataaccgc gtcgcggatg accagggctt tttacgccag
    27781 tggagcaagg tggcgaaaga acgcaaactg caacggctct atatcggcga accgagcgcc
    27841 gaagccgttg ccgcgcaaat gccggatctg attttaatta gcgcaactgg cggggattcg
    27901 gcgctggcgc tgtatgatca gctttccacc atcgccccga cattaatcat caattacgac
    27961 gacaaaagct ggcagtcgct gttaacgcag cttggcgaaa ttaccgggca tgagaaacaa
    28021 gcggcagagc ggattgcgca gtttgataag caactggcgg cggcgaaaga gcaaatcaaa
    28081 ttaccgccgc agccggtcac tgccattgtc tatactgccg ctgcacacag tgccaatctc
    28141 tggacgccag aatcagcaca agggcagatg ctggaacaac tcggctttac gctggcgaag
    28201 ttacccgcag ggttaaatgc cagccaaagt cagggtaaac gccatgacat cattcagctt
    28261 ggtggggaaa atctggctgc agggttaaat ggcgagtcac tattcctgtt cgccggtgat
    28321 cagaaagatg ccgatgctat ttatgctaat ccgctgctcg cgcacctgcc tgcagtacaa
    28381 aacaagcagg tttatgcgct gggaaccgag acgttccgtc tggattacta cagcgccatg
    28441 caagtgctgg ataggcttaa ggcgctgttt taagcattaa ctgtcggacg ctgtcacctg
    28501 cggcggcgtc tggcgaaaat gtcgcaactc caccagcacc agcaataaca acacgccgat
    28561 aatcaacaaa ccaaaaccgc tcgcgcttgc ggaagcaacc ggtgtcatca tcgcgcccaa
    28621 accacccagc agcgccgcgc ctatggcatc gcccgtcacg ttctgcgccg tccacaaacc
    28681 gttaatccgc cctaacatcg cttccggggt ttgcgtttgc agcattgtgt attgcagcaa
    28741 cgagctgacc gcactcaacc agccgaacag cgccagacaa accacgccta aaatccacat
    28801 cggcatcagg ccaaacagac caatggcgag gaacgatccc agcgtggaga gcagcatcaa
    28861 taaccctggt cgcgcgctat gcgccagctt cccgctggtt aacgcgccaa tagccgcgcc
    28921 gagcgggatc gccgcgtaga gaaaaccaat ctgtgccgct gacatctgcc agttgtcagc
    28981 cagcgccgga tacagtaccc gcaccgcgct cgccatcgtc aataaaccac ccagcagcgc
    29041 aatccctcct accagcgggc tggcgagcag aaaacgaaat cctgccagta atgatttcaa
    29101 cggatgctcg cgcggctgcg gtggcggtgg caccgccgga aggcttaaca acggtagcaa
    29161 ggtaataaac gtgcccgccg ccgccagccc gtagttccag gctacgccgc cggtcgccag
    29221 caataaaccg ccaatcatgg gcgaaatcac cgaccccaga cgcacggtca acatggtgat
    29281 cgccccggcc tgcattaagt tttcacgccc taccagtgct ggtgtcgccg ccaatagcgc
    29341 cgtaacgcca agcgatgcga aaaaaccatc ccataaacca agtaaataga ttgccagcaa
    29401 tgacggctcc ggcagcagtg cattaaggca cagtccaatg aagccaatgc cacaggtgcc
    29461 gcgcgccagc aaaatcactt ttttgcgctc atagcgatcc gccagcacac cgccaaccat
    29521 caggccaaca aacatcgcgc cgccggtcag cgtcaccgaa agccccacct gccaggtaga
    29581 atgcgtcatc atctggatct gcaccggcac cgcgacgccg agcaaaccca gagacacaat
    29641 tgagataaaa cgagcgagga atactgcgcg aaacgccggg tgcgttttca acaggctgag
    29701 gttaagcagc caggattgtt tattcattac aatgccttgc catcaaatat tttaaatacc
    29761 tattcttagg ctgcacatgc taacatatcc aaataagatc gataacgata attaatttca
    29821 ttatcatgga agttcgtatg tctggttctg ttgccgtgac acgcgccatt gccgtgcccg
    29881 gattgctgtt attactgatt atcgcgacgg cattaagcct gctcattggg gcaaaatcac
    29941 tccccgcttc cgtagtgctg gaggccctct ccggcacctg ccagagcgcc gactgcacca
    30001 tcgtgctcga cgcgcgactg ccgcgcaccc ttgccggttt acttgcaggc ggcgcgcttg
    30061 gccttgccgg ggcgttgatg caaaccctca cccgaaatcc acttgccgac cccggcttgc
    30121 ttggcgtgaa tgccggagcc agctttgcca ttgtgctggg tgcggcgctg tttggttact
    30181 catccgcgca ggaacaactg gcgatggcct tcgccggggc gctggtggcc tcattgattg
    30241 ttgcctttac cggcagtcag ggtggagggc agttaagtcc ggtgcgttta accctggcgg
    30301 gcgtggcgct ggcggcggtg ctggaaggac tgaccagcgg catcgccctg cttaatcctg
    30361 acgtctacga tcagttgcgt ttctggcaag ccggttcgct ggatattcgc aatctacata
    30421 ccttaaaagt ggtgctgatc ccggtgctga tcgccggagc aactgcgcta ttactgagtc
    30481 gcgcgctgaa cagtttgagc ctcggcagcg acaccgcgac ggcgctgggc agtcgcgtgg
    30541 cgcgcacaca gttgattggt ctgctggcga ttaccgtgct ttgtggtagt gcgacggcag
    30601 tagttggccc gattgccttt attggcctga tgatgccgca tatggcgcgt tggctggtgg
    30661 gtgccgatca tcgctggtcg ctgcccgtca cgctacttgc tacccctgcc ctgctgctgt
    30721 ttgccgatat catcgggcgg gtgattgtgc ccggcgaact gcgcgtttct gtggtcagtg
    30781 cgtttattgg tgcaccggtg ctgatcttcc tcgtgcgacg taaaacgcga ggtggtgcat
    30841 gatttacgtc tctcgccgat tactcatcac ctgtttgctg ctggtttccg cctgtgtggt
    30901 tgcaggtatc tggggattac gcagcggtgc cgtcacgctg gaaacctcgc aggtattcgc
    30961 cgcgctgatg ggcgatgcgc cgcgcagtat gacgatggtg gtcaccgaat ggcgtttacc
    31021 acgcgtgctg atggcgctgt tgattggcgc agcactgggc gtcagtggcg cgatttttca
    31081 gtcgctgatg cgtaacccgc tcggcagccc tgacgtaatg ggctttaaca ccggggcgtg
    31141 gagcggcgtg ctggtggcga tggtgctgtt tggtcaggac ctgacggcta tcgcgctggc
    31201 agcaatggtg ggcggcatta tcacttcgct gttggtctgg ctgctcgcct ggcgtaacgg
    31261 tatcgacacc tttcggttga ttattatcgg tatcggcgtt cgcgccatgc tggtggcctt
    31321 taatacctgg ctgttgctga aagcgtcttt agaaacggcg ctaacagcag gtttgtggaa
    31381 tgccggatcg ctcaacggcc tgacgtgggc aaaaacctcg ccttccgcac ccatcattat
    31441 attgatgctc attgccgccg ccttgctggt gcggcgaatg cgcttgctgg aaatgggcga
    31501 tgataccgcg tgtgcgctgg gcgtcagcgt cgaacgttcg cgtctgttaa tgatgctggt
    31561 tgcagtggtg cttaccgctg cggcaacagc gcttgccggg ccgatttcct ttattgcttt
    31621 agtcgcgccg cacattgccc gacgcattag cggcaccgct cgctgggggc taacccaggc
    31681 ggcgctgtgc ggggcgttgt tactgctggc ggccgatctt tgcgctcagc aactgtttat
    31741 gccgtatcaa cttccggttg gcgtcgttac cgtcagcctc ggcggtattt accttatcgt
    31801 cttgttaatt caggagtctc gcaaaaaatg accgaatcag tagcccgttt gcgcggcgaa
    31861 cagttaaccc tcggatatgg caaatatacc gttgcggaaa atctgactgt agaaatacct
    31921 gatggtcact tcacggcaat tatcgggcca aatggctgcg gtaaatccac gttactgcgt
    31981 accttaagcc gcctgatgac gcctgctcat gggcatgtct ggctggatgg cgagcacatt
    32041 caacattacg ccagtaaaga ggttgcacgc cggattggtc tgttggcgca aaatgctacc
    32101 acgccgggcg atatcaccgt gcaggagctg gtggcgcgtg gacgttatcc gcatcaaccg
    32161 ctgtttaccc gctggcgcaa agaggatgaa gaagcggtaa cgaaagcaat gcaggccacg
    32221 ggaataactc atctggcaga tcaaagcgtg gatacccttt ctggcggaca acgccagcga
    32281 gcgtggatcg cgatggtgct ggcccaggaa acggcaatta tgctgctcga cgaacccacg
    32341 acctggctgg atatcagtca tcagattgat ttgctggagt tgttaagcga actgaaccgc
    32401 gaaaaaggct ataccctggc ggcggtgctg cacgatctta atcaggcctg tcgttacgcc
    32461 agccatttga ttgcattgcg ggaagggaaa attgttgctc agggagcgcc gaaggagatt
    32521 gtcactgctg aactgattga gcgcatttat ggtctgcgct gcatgatcat tgacgatcca
    32581 gttgccggaa cgccgcttgt ggtgccgctc ggacgaacgg caccttcaac cgcaaatagt
    32641 taaactaagt ggtctgccat catggcatcc tgttttctgg atgccatcgc atagcgcaac
    32701 agcacgccac cacaagccac catcccgccg atcaacgcgg aaaggatcac aataatcgcc
    32761 ttacccggac cgtctttttt cactggcaat gacgggctta actgatattt aaacggcgta
    32821 aaattcacat cgttgacatg tgcttttgtt aattgctcga caagatactg ccgattacgt
    32881 aattcaccgt tcagttccgc aacgtcagtg accgcttttt ctatttccag tttgcgttca
    32941 ataccgtctg caccgagaga aatagaaaaa tcggggtcat ctttaacggc ctgaccatta
    33001 ctgtaaacgg gctttttaat tcctgccgcg ttggcaatgt cgagtgaata attgaggcgc
    33061 tgaatgtttg catcaagttg atttttcgtt ttaatgcgat cctgagccag tttttctttt
    33121 tcaaactggg ttttgatctc cagtttatta cggacgtttt ctagcgactc tttcaccacc
    33181 aacgtagaga tataatcgat atacccgctc aaaacggtct gcgcctcttc actggttggc
    33241 gcggtaaaac ttagcgtcca ggaggtatac agtgacggtt catctttttt cttactggca
    33301 ttgtcatcaa ccgctttcat tttttcgctc aatgcgacaa ttgcgcgatg caaatccagt
    33361 tcgtcgattt tcgcctcttt taattggtcc atcacataag gtgatgaacg caggtactct
    33421 tccagcaagc taaccgactg aaacttcttg ataaacaggt taaatgcttc tgtacgatca
    33481 attttgatat ccagatccag cacacgaagc ttagtgaatg atttctccaa ctcttgccac
    33541 tgaacaggtt ctggaggcgt gacaaccgcc gcgctggtcc atttttgcgg caggatgaaa
    33601 gagatcagca agcctgcgca ggcaaacgca aaaacgaccg ccatgaccgt ttttttggcc
    33661 cgccataaaa ctgagattag attaagtaaa tcaatttcat tattactggg cgacgccaga
    33721 ggataatcgg gaaaatgagc gtcacttccc tgtttaatat tcagtgatga catggggtat
    33781 aacctgaaaa tgagtccaat agggggaaa
//
LOCUS       DAJOSY010000043        33663 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJOSY010000043 DAJOSY010000000
VERSION     DAJOSY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.043"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(64..2133)
                     /gene="glyS"
                     /locus_tag="OHN27_002123"
     CDS             complement(64..2133)
                     /gene="glyS"
                     /locus_tag="OHN27_002123"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCQ2286562.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFTEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2143..3054)
                     /gene="glyQ"
                     /locus_tag="OHN27_002124"
     CDS             complement(2143..3054)
                     /gene="glyQ"
                     /locus_tag="OHN27_002124"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCQ2286563.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3149..3448)
                     /locus_tag="OHN27_002125"
     CDS             complement(3149..3448)
                     /locus_tag="OHN27_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCQ2286564.1"
                     /translation="MMMNAFFPAMALMVLVGCSIPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3623..4618
                     /gene="wecH"
                     /locus_tag="OHN27_002126"
     CDS             3623..4618
                     /gene="wecH"
                     /locus_tag="OHN27_002126"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCQ2286565.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4660..5097)
                     /locus_tag="OHN27_002127"
     CDS             complement(4660..5097)
                     /locus_tag="OHN27_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCQ2286566.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5143..5484)
                     /locus_tag="OHN27_002128"
     CDS             complement(5143..5484)
                     /locus_tag="OHN27_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286567.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFTHICQLVALITIGLLFIGVLNAPINTYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            complement(5653..7107)
                     /gene="xylB"
                     /locus_tag="OHN27_002129"
     CDS             complement(5653..7107)
                     /gene="xylB"
                     /locus_tag="OHN27_002129"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCQ2286568.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQLLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMV"
     gene            complement(7179..8501)
                     /gene="xylA"
                     /locus_tag="OHN27_002130"
     CDS             complement(7179..8501)
                     /gene="xylA"
                     /locus_tag="OHN27_002130"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCQ2286569.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8867..9859
                     /gene="xylF"
                     /locus_tag="OHN27_002131"
     CDS             8867..9859
                     /gene="xylF"
                     /locus_tag="OHN27_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2286570.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9937..11478
                     /gene="xylG"
                     /locus_tag="OHN27_002132"
     CDS             9937..11478
                     /gene="xylG"
                     /locus_tag="OHN27_002132"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCQ2286571.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11456..12637
                     /gene="xylH"
                     /locus_tag="OHN27_002133"
     CDS             11456..12637
                     /gene="xylH"
                     /locus_tag="OHN27_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCQ2286572.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12715..13893
                     /gene="xylR"
                     /locus_tag="OHN27_002134"
     CDS             12715..13893
                     /gene="xylR"
                     /locus_tag="OHN27_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCQ2286573.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYHGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14089..14913)
                     /locus_tag="OHN27_002135"
     CDS             complement(14089..14913)
                     /locus_tag="OHN27_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCQ2286574.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15233..17263
                     /gene="malS"
                     /locus_tag="OHN27_002136"
     CDS             15233..17263
                     /gene="malS"
                     /locus_tag="OHN27_002136"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCQ2286575.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIM
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLLLKQGYGFVREHGDDKVLVVWAGQQ"
     gene            17441..18694
                     /gene="avtA"
                     /locus_tag="OHN27_002137"
     CDS             17441..18694
                     /gene="avtA"
                     /locus_tag="OHN27_002137"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCQ2286576.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18845..19318)
                     /locus_tag="OHN27_002138"
     CDS             complement(18845..19318)
                     /locus_tag="OHN27_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCQ2286577.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREQGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19420..20268)
                     /gene="yiaJ"
                     /locus_tag="OHN27_002139"
     CDS             complement(19420..20268)
                     /gene="yiaJ"
                     /locus_tag="OHN27_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCQ2286578.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20469..21467
                     /gene="yiaK"
                     /locus_tag="OHN27_002140"
     CDS             20469..21467
                     /gene="yiaK"
                     /locus_tag="OHN27_002140"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCQ2286579.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21479..21946
                     /locus_tag="OHN27_002141"
     CDS             21479..21946
                     /locus_tag="OHN27_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCQ2286580.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYTQIIDLTTREAVVNRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCIMQTASEIRKIVVKVALTALN"
     gene            22064..22537
                     /gene="yiaM"
                     /locus_tag="OHN27_002142"
     CDS             22064..22537
                     /gene="yiaM"
                     /locus_tag="OHN27_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCQ2286581.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22540..23817
                     /gene="yiaN"
                     /locus_tag="OHN27_002143"
     CDS             22540..23817
                     /gene="yiaN"
                     /locus_tag="OHN27_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCQ2286582.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            24673..26145
                     /gene="lyxK"
                     /locus_tag="OHN27_002144"
     CDS             24673..26145
                     /gene="lyxK"
                     /locus_tag="OHN27_002144"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HCQ2286583.1"
                     /translation="MKEAGVEVITDLDREGREAGVQRLPLCALSPQPGWAERDMAELW
                     QCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSSDRRAMEIV
                     RRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTHDYLRWCLT
                     GVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEICGEITAQTA
                     ALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGLRDGEAHPY
                     VYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAGGDLFFLPF
                     LYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFTDVHTLRVT
                     GGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYHNFSEAQRDLRHP
                     VRTLLPDMTAHQLYQKKYQRYQHLIAALQGFHARIKEHTL"
     gene            26142..26804
                     /gene="ulaD"
                     /locus_tag="OHN27_002145"
     CDS             26142..26804
                     /gene="ulaD"
                     /locus_tag="OHN27_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCQ2286584.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            26797..27657
                     /locus_tag="OHN27_002146"
     CDS             26797..27657
                     /locus_tag="OHN27_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCQ2286585.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27651..28346
                     /gene="araD"
                     /locus_tag="OHN27_002147"
     CDS             27651..28346
                     /gene="araD"
                     /locus_tag="OHN27_002147"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCQ2286586.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28378..29193)
                     /locus_tag="OHN27_002148"
     CDS             complement(28378..29193)
                     /locus_tag="OHN27_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2286587.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFQKHTGLTPAAWKRRYSKEHINS"
     gene            29419..30819
                     /locus_tag="OHN27_002149"
     CDS             29419..30819
                     /locus_tag="OHN27_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2286588.1"
                     /translation="MTSTPITTADIANGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVVVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            30830..32800
                     /locus_tag="OHN27_002150"
     CDS             30830..32800
                     /locus_tag="OHN27_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCQ2286589.1"
                     /translation="MNISEVDLRKLTVSDPFLGQYQQLVRDVVISYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIAYAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDRVFGPDEDKLQGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFAYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGNGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(32930..33649)
                     /locus_tag="OHN27_002151"
     CDS             complement(32930..33649)
                     /locus_tag="OHN27_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HCQ2286590.1"
                     /translation="MALFALPFMASATASELSIGAGAAYNESPYRGYNENTKAIPLIS
                     YEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPTDNDDYAMQQLDKRDSTAMA
                     GVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGVLYYDENF
                     SDYYYGISESDSRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYSELPEEIT
                     DSPMIDRNESFTFVTGVSWRF"
BASE COUNT         8188 a         8743 c         8496 g         8236 t
ORIGIN      
        1 ctgtattatt tagcgataat aaaaaaagcc tgccagatgg caggcttttt aataacggcg
       61 ttattactgc aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca gtttctccag
      121 catggtcaga cggttgatac gcaattcttt gtcatcaacc attaccatca ctttatcgaa
      181 gaaagcatca accggttcac gcagctcagc cagttcgacc agcgcatcct ggtaacgacc
      241 ttccgtaaag tacggctcca gcttgtcacg tagcaccaca acctgcatcg ccagtttaat
      301 ttcttccggc tctttcaggg tagaggcatt cacgcggtcg ctcagcactt cgtcagattt
      361 cgccagaatg ttagatacac gtttgttcgc cgccgccagt gcagcagctg catccagggt
      421 acggaaatgc gataccgctt tcatacgggc atcgaaatca gccggacgag tcggacgacg
      481 cgccagtacc gcctggatgg tatcaacggt ataaccttcg tcctgatacc aggcgcggaa
      541 gcgaccgagc ataaagtcga taacatcatc aactacgttg gcattagtca gcttatcgcc
      601 atacagacgc accgcttctt cggtcagcgt ttgcagatca aggttgaggt tcttctcaac
      661 gataatacgc agcacgccaa gcgcggcacg acgcagcgca aacgggtctt tgtcgccttt
      721 cggatgctga ccgataccga agatacccgc cagggtatcc atcttgtcag caatcgccag
      781 cgcacaagct actgggttgg acggcaggtc atcaccagca aaacgcggct gatactgctc
      841 attcagcgcc accgcgacat cttccgcttc gccatcgtga cgcgcatagt gcatccccat
      901 aacgccctgg gtgtcggtga actcgaagac catgttggtc atcaggtcgc acttagacag
      961 cagacccgca cgggtagcgt ggttaacgtc agcgccaatc tgttcagcaa tccagccagc
     1021 cagcgcctgg atgcggtcag ttttgtcgcg cagcgtcccc aactgttgct ggaacaacac
     1081 ggtttgcagg cgcggcaggt tatcttcaag acgtttttta cggtcggtgt tgaagaagaa
     1141 ctcggcatcc gccagacgcg gacgaacgac tttctcgtta ccggagataa tctgctgcgg
     1201 atctttcgat tcgatgttgg caacgaagat aaagttcggc agcagtttgc cgtcgttcgc
     1261 atacaccggg aagtatttct ggtcaccttt catggtgtaa accagcgctt cagccggcac
     1321 cgcgaggaat ttctcttcga attttgcggt cagaacgacc ggccactcca ccagcgaagc
     1381 cacttcttcc agcaggcttt cgcttaagtc agcgttacca ccaatcttac gcgctgcttc
     1441 ttcggcatcg gctttaatct tcgccttacg ttcttcgtaa tcggcgatga ctttcccacg
     1501 ctcacgcaga atttccggat actgatcggc gttatcgatg gtgaattccg gctcgcccat
     1561 aaagcggtgg ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg ccggaatgac
     1621 tttgtcgccc agcagcaggg tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc
     1681 gccccaacgc atcagtttcg ggatcggcag tttcgccaga gaagtcgcaa ccatattcgg
     1741 cagcagtgct tcggtgcttt cgcccttcac atgggcgcga tacagcagcc attcgccttt
     1801 atcggtagtc agacgctcag cctggtcaac ggtaataccg caaccacgcg cccaaccttc
     1861 tgccgctttg ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt
     1921 ttcgatttca cgatccggtt gcgcttccgc caggttagct actttcagcg ccagacgacg
     1981 cggagcagca aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt
     2041 aaagttcgca gcaaaggact cagccaggct gcgcagtgct tttggtggca gctcttcagt
     2101 gccgatttcc accagaaaag ttttctcaga catagccgcc tcttacttat ctttgttgca
     2161 catcgggaag ccgagggctt cacgggaagc gtagtatgct tctgccactg ctttggtcag
     2221 ggtgcgaatg cgcagaatgt agcgctgacg ctcggtgacg gagatggctt tacgcgcatc
     2281 cagcaggttg aagctgtggg cggctttcag aatacgctcg taggctggca gcggcagcgg
     2341 attttccagc gccagcagct gctgcgcttc tttctcgtac tgctcgaagc aggtgaacag
     2401 gaagtccaca tccgcgtatt cgaagttgta agtggactgc tccacttcgt tctgatggaa
     2461 cacgtcgccg taggtggttt tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct
     2521 gtctacgccc tgaatgtaca tggccagacg ttccagaccg taggtgatct cgccggtaac
     2581 cggtttacac tccagaccac caacctgctg gaagtaagtg aactgcgtca cttccatgcc
     2641 gttcagccac acttcccagc ccagtcccca ggcacccagc gtcgggtttt cccagttatc
     2701 ttccacgaaa cggatgtcgt gaatagtcgg gtccatgccc agctctttca gagaaccgag
     2761 gtacagctcc tgaatattgt ccggcgatgg cttaatgacc acctggaact gatagtagtg
     2821 ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc gggcgacgag aaggctgaac
     2881 ataagcagcc gccatcggtt ctggccccag cgcgcgcaga caggtcattg ggtgagaggt
     2941 tcccgcgccg acttccatgt ccaatggttg aacaatggtg cagccctggc gagcccagta
     3001 atcctgtaag gtcaggatca agccctggaa ggtcctggta tcaaactttt gcatattatt
     3061 tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta tacccgctgg atggaagata
     3121 tacagtacga aacgggaaaa agcagggctt aacgcatgga aagatgcaaa aaatggccat
     3181 ccgcgtcaaa agaacagaca aactgctctt tacggaaggt ataaccgcgc atttcatagc
     3241 ttccctggaa ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg ttataacgtt
     3301 ttgccgcctg atccttgcat aacgcttcca tattcaacga acgcagagga tcaaccttta
     3361 cccgttgtgc tttctgcacg ggtgacggta tagaacaacc cactagcacc ataagcgcca
     3421 ttgccggaaa gaaagcgttc atcatcatta aattaccgtc tggtttgcag gtgagtctta
     3481 ttatttatat cggtatagtg gctgatatct tacgaatttc cttaagcctc tataagctgt
     3541 agtgataaaa ctcagattat tccagtgcgc caaccaaatg atttatcggt cagacttttt
     3601 ttaaccagat acagagacac agatgcagcc caaaatttac tggattgata acctgcgagg
     3661 gatagcgtgt ttaatggtgg tgatgattca caccactacc tggtatgtga ccaatgctca
     3721 tagtgttagc cccgtcacat gggatatcgc caatgttctg aattctgcct ctcgtgtcag
     3781 cgtgccgcta tttttcatga tttccggcta tctctttttt ggcgaacgca gcgcccagcc
     3841 gcgccatttc ttgcgtatcg gcttatgtct gattttttat agcgcaatcg cactgctcta
     3901 cattgcgctg tttacctcca tcaatatgga gttagcgctg aaaaacctgc tgcaaaagcc
     3961 agtgttttac cacttgtggt ttttcttcgc gattgcggtg atttatctgg tttcaccgct
     4021 gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg ctaatggcgg tgattggcat
     4081 tatcgctaac ccaaacacag tgccgcagaa aattgacggt tttgaatggc tgccaattaa
     4141 cttatatatc aatggcgata ctttttacta cattctgtat ggcatgttgg gccgcgctat
     4201 agggatgatg gacacacagc ataaagcact gtcgtgggtg agcgccgcgc tgtttgcgac
     4261 gggggttttt attatctctc gcgggacatt atatgaattg cagtggcgcg gaaattttgc
     4321 cgatacctgg tatctttact gtgggccgat ggtttttatc tgcgcaatcg cgctattgac
     4381 tctggttaaa aacacgctgg atacgcgtac cattcgcgga cttggcttaa tctcccgcca
     4441 ttcattgggt atatacggat tccacgcctt gattatccat gcgctgcgca cccggggaat
     4501 tgagcttaaa aattggccaa tactggatat tatttggatc ttttgcgcga cgttggcagc
     4561 gagtttgtta ctttctatgc tggtacaacg aatcgacaga aacagattag tgagttaagt
     4621 aaaagcccgg tcacattgga ctgaccgggc ttacgtgagt tattcgctgt attcttcctg
     4681 ggtaacctgt gtttcttttg gtgggtttat tccggcatca cgaatatttt tctgcaccgc
     4741 tactgcacca aacaagctta agaagaaagc cagtccataa aaaccttttt cgctgagtaa
     4801 taatgtcgca ttccacagac ctaccatcag taatgcaaca gagataataa agacagtcag
     4861 gcaggtcata taataaatgg aagtggtcgg tatgccttca tacttgtccc gaacggtctt
     4921 ttgataagac gccgcagaaa acagtcctaa taccagtacg gcaaaataat atcctttttc
     4981 atttaactgc atctctgcat tccatagccc taacagatag gtaacgatac caccaacgag
     5041 agctatccat gacacaatac taaaggccgg tgaataggtt gatattttgt tgtccatcac
     5101 agtattcctt tcattcctga atatgtaaga gctttatgtt gctcatgccg agcgaaacaa
     5161 acgcatttga ccaaacaagc agacaaaaaa ggcgatggga tagatcacca tttcataagt
     5221 attgataggt gcgtttaaaa caccgataaa caagagaccg atagtgatta acgcaactaa
     5281 ctggcagata tgggtaaaaa aatcatccag ttgcccgagt ttttctgcgc gaagatatgc
     5341 atagttgcca aaagttgctg tcattaacac gccaagaaaa tagccttttc cacttaacaa
     5401 tgggcatgag atccatagcc caaccagata aaccagcgcc ccgacaacaa ataatagttt
     5461 tgccacagtc tttgaggttt tcataacgat ctccatatct accagcgata cattacgagt
     5521 aaccaacgaa agacaaaact gaaaaatgcc attaacaaat gattttcaga ataaattcat
     5581 actaaatatt aattaattac tgaaatatat agatgtgaat tatcccccac ccggccaggc
     5641 aggggataac gtttacacca ttaatggcag aagttgctga tagaggcgac ggaacgtttc
     5701 acgtcgtggc tgataagcgg cataacgctg cgcatctggt agatgcgact gttctaacgg
     5761 cagttgcggc aacaactcaa tgagcgattt ctctggattc gccgcaatct gcgccagcct
     5821 tgctgcgccc agtgctggcc ccacatcccc ccccgtacgg taatcgagca gctgaccgct
     5881 gatatccgcc aacatctgac gccagtactc actacgcgcc ccgccaccaa tcaacgtaac
     5941 actttgcggt ttaatgccgc aggcatgcac gacatccatg ccatctgcca gcgcataacc
     6001 cacgccttcc agcactgctc gcgccagttc attggggcca tgttgatgag tcaaaccaaa
     6061 gaaaaccccc ttcgcctggg gattattgtg tggcgtacgc tcgccggaaa gataaggcag
     6121 aaaccaaact ggctcggcac tttcatcagc ctgttgagct gcagcgatta aagctggaac
     6181 attgctcagg ccggttaatt tcgcggccca atccagacac gacgccgcac tcagcattac
     6241 agacattaaa tgccaacgtt gcggtagcgc atggcaaaag ctgtgtacgg cgctttctgg
     6301 cttgcttaag aacccttcgc tgacagcaaa atagaccccc gacgtcccca gcgataacat
     6361 tgcctgatta gcatcaacca ttcccacacc aaccgcacca gctgcattgt cgccaccgcc
     6421 tgcgacaact ggcaccgtcg ccatacccca cgctttcgca acttcaggta acaaagcacc
     6481 agtaatttcg ctgccttcgt ataatgcggg catctggtca cgagataagt cgcaagcctg
     6541 cagcatgacg tcactccagt cacgctttgc gacatccagc cacatggtgc cagctgcgtc
     6601 agacatatcg ctggcaaact cccccgtcat acgcagacgc aagtaatctt tcggtaataa
     6661 tactttgtcg atttgacgga atatctccgg ctcatgccgc tgaacccata gcaatttagg
     6721 cgcagtaaat ccgggcatca tcaggttgcc ggtaatcact cgtgattgcg gaactctcgc
     6781 ttccagcaaa gtgcactctt gcgcacagcg cccgtcgttc cacaaaatgg cagggcgcaa
     6841 tacccgttgt tgagcatcca gtaaggttgc tccatgcatc tggccggcaa tacccaatgc
     6901 tttaacgtcc tgcagagaat gctgatcgcc cagagctttc attgcgcgat cagttgcctg
     6961 ccaccactgt tccgggtctt gttccgacca gagtggatgc gggcgcgaaa cggtcagctt
     7021 ttccgtttgc gaagcaacca cctcaccctg ctcgttgagc aaaataactt ttacgcccga
     7081 ggtgccaaga tctatcccga tatacatatc gatcgttcct taaaaaaatg cccggtatcg
     7141 ctaccgataa ccgggccaac ggactgcaca gttagccgtt atttgtcgaa cagataatga
     7201 tttaccagat tttccagttg ctcctggcga ccactctgat gcaccggaga caaattatgt
     7261 tcctgagcat atttggctaa atctgccagt gacatttggc ctttcaggat ttgctggccc
     7321 aattcgctat tccagccgga ataacgctgc gcgatgcgtt tatccagctc gccatcttca
     7381 atcatgcgcg ctgcaatttt cagcgccagt gccatcgtat ccatcgcgcc gatatgaccg
     7441 taaaacagat catatttatc agtactttga cgacgtactt tggcatcgaa gttcagacca
     7501 ccggtggtga aaccgcctgc tttgagaatt tcatacatca ccagcgcatt ctcttccaca
     7561 ctgttcggga actggtcggt gtcccagccc agttgcgcat cgccacggtt ggcgtcgaca
     7621 gaaccgaaca ggccaagcgc aatggcggtg gctatttcat gatggaaaga gtgacctgcc
     7681 agcgtcgcgt ggttagcttc aatgttcagt ttaatctctt tttccagacc aaactgtttc
     7741 aggaagccat agaccgtcgc ggcatcgtaa tcatattgat gtttagtcgg ttcttgcggt
     7801 ttcggttcga taagcaacgt gccctggaag ccgattttat gtttatgctc aaccaccatc
     7861 tgcataaagc ggcccagttg ttcacgctcc tgacgcaagt cggtatttaa cagcgtttcg
     7921 taaccttcac gaccacccca caggacatag ttttcaccgc ccaatttatg ggttgcttcc
     7981 atcgctgtaa caacttgcgt tgccgcccag ctgaagactt caggatctgg gttcgtcgcc
     8041 gcacccgcgc catagcgagg gtttgtaaag cagttagcgg ttccccacag cagcttcacg
     8101 ccgctctctt cttgcttggc tgccaggaca tcaaccattt gcgcaaaatt attgatgtac
     8161 tcttttaacg acgcgccctc aggggaaaca tccacatcgt ggaagcaata aaatggcaca
     8221 tgtaacttgt ggaaaaactc aaatgcgaca tctgctttac gcttcgccaa cgccagtgcc
     8281 tcaccaggct gctgccacgg acgattaaac gcccccacac caaacatatc cgccccgttc
     8341 cagcagaagg tgtgccagta gcaggcggca aaacgcaagt gctcttccat acgcttaccc
     8401 aacaccagtt cgtcgggatt gtagtgacgg aatgctaacg ggtttgagga ttttgagcct
     8461 tcataacgaa cgcgatcgag ctggtcaaaa taggcttgca tattgaactc cataatcagg
     8521 taatgccgcg ggtgatggat gatgtcgtaa tattgggcac tccctttcag ttgctcaatt
     8581 atgttatttc acactgctat tgagataatt cacaagtgtg cgctcgctcg caaaataaaa
     8641 tggaatgatg aaactgggta attcctcgaa gagaaaaatg caataagtac aattgcgcaa
     8701 caaaagtaag atctcggtca taaatcaaga aataaaccaa aaatcgtaat cgaaagataa
     8761 aaatctgtaa ttgttttccc ctgtttagtt gctaaaaatt ggttacgttt atcgcggtga
     8821 ttgttactta ttaaaactgt cctctaacta cagaaggccc tacaccatga aaataaagaa
     8881 cattctactc accctttgca cctcactcct gcttaccaac gttgctgcac acgccaaaga
     8941 agtcaaaata ggtatggcga ttgatgatct ccgtcttgaa cgctggcaaa aagatcgaga
     9001 tatctttgtg aaaaaggcag aatctctcgg cgcgaaagta tttgtacagt ctgcaaatgg
     9061 caatgaagaa acacaaatgt cgcagattga aaacatgata aaccggggtg tcgatgttct
     9121 tgtcattatt ccgtataacg gtcaggtatt aagtaacgtt gtaaaagaag ccaaacaaga
     9181 aggcattaaa gtattagctt acgaccgtat gattaacgat gcggatatcg atttttatat
     9241 ttctttcgat aacgaaaaag tcggtgaact gcaggcaaaa gccctggtcg atattgttcc
     9301 gcaaggtaat tacttcctga tgggcggctc gccggtagat aacaacgcca agctgttccg
     9361 cgccggacaa atgaaagtgt taaaacctta cgttgattcc ggaaaaatta aagtcgttgg
     9421 tgaccaatgg gttgatggct ggttaccgga aaacgcattg aaaattatgg aaaacgcgct
     9481 aaccgccaat aataacaaaa ttgatgctgt agttgcctca aacgatgcca ccgcaggtgg
     9541 ggcaattcag gcattaagcg cgcaaggttt atcagggaaa gtagcaatct ccggccagga
     9601 tgcggatctc gcaggtatta aacgtattgc tgccggtacg caaactatga cggtgtataa
     9661 acctattacg ttgttggcaa atactgccgc agaaattgcc gttgaattgg gcaatggtca
     9721 ggagccaaaa gcagatacct cactgaataa tggcctgaaa gatgtcccct cccgcctcct
     9781 gacaccgatc gatgtgaata aaaacaacat caaagatacg gtaattaaag acggattcca
     9841 caaagagagc gagctgtaag cgttacgccc cggcgcggag cgggggcgtg atttctctcc
     9901 atgccgcgtg aatgaattgg cttaggtgga gtcgttatgc cttatctact tgaaatgaag
     9961 aacattacca aaaccttcgg cagtgtgaag gcgattgata acgtctgctt gcggttgaat
    10021 gctggcgaaa tcgtctcact ttgtggggaa aatgggtctg gtaaatcaac gctgatgaaa
    10081 gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg aaattatttt tgcgggagaa
    10141 gagattcagg cgagtcacat ccgcgatacc gaacgcaaag gtatcgccat cattcatcag
    10201 gaattggccc tggtgaaaga attgaccgtg ctggaaaata tcttcctggg taacgaaata
    10261 acccacaatg gcattatgga ttatgacctg atgacgctac gctgtcagaa gctgctcgca
    10321 caggtcagtt tatccatttc acctgatacc cgcgttggcg atttagggct tgggcaacaa
    10381 caactggttg aaattgccaa ggcacttaat aaacaggtgc gcttgttaat tctcgatgaa
    10441 ccgacagcct cattaactga gcaggaaacg tcggttttac tggatattat tcgcgatcta
    10501 caacagcacg gtatcgcctg tatttatatt tcgcacaaac tcaacgaagt caaagcgatt
    10561 tccgatacga tttgcgttat tcgcgacgga cagcacattg gtacgcgtga tgctgccgga
    10621 atgagtgaag acgatattat caccatgatg gtcgggcgag agttaaccgc gctttaccct
    10681 aatgaaccac ataccaccgg agatgaaata ttacgtattg aacatctgac ggcatggcat
    10741 ccggtcaatc gtcatattaa acgagttaat gatgtctcgt tttccctgaa acgtggcgaa
    10801 atactgggta ttgccggact cgttggtgcc ggacgtaccg agaccattca gtgcctgttt
    10861 ggcgtgtggc ccggacaatg ggaaggaaaa atttatattg atggcaaaca ggtagatatt
    10921 cgtaactgtc agcaagccat cgcccagggg attgcgatgg tacccgaaga cagaaagcgc
    10981 gacggcatcg ttccggtaat ggcggttggt aaaaatatta ccctcgccgc actcaataaa
    11041 tttaccggtg gcattagcca gcttgatgac gcggcagagc aaaaatgtat tctggaatca
    11101 atccagcaac tcaaagttaa aacgtcgtcc cccgatcttg ctattggacg tttgagcggc
    11161 ggcaatcagc aaaaagcgat cctcgcccgc tgtctgttac tcaacccgcg cattctcatt
    11221 cttgatgaac ctaccagggg tattgatatt ggcgcgaaat atgagatcta caaattaatt
    11281 aaccaactcg tccagcaggg tattgccgtt attgtcatct cttccgaatt acctgaagtg
    11341 ctcggcctta gcgatcgtgt actggtgatg catgaaggga aactaaaagc caacctgata
    11401 aatcataacc tgactcagga gcaggtgatg gaagccgcat tgaggagcga acatcatgtc
    11461 gaaaagcaat ccgtctgaag tgaaattggc cgtaccgaca tccggtggct tttccgggct
    11521 gaaatcactg aatttgcagg tcttcgtgat gattgcagct atcatcgcaa tcatgctgtt
    11581 ctttacctgg accaccgatg gtgcctactt aagcgcccgt aacgtctcca acctgttacg
    11641 ccagaccgcg attaccggca tcctcgcggt aggaatggtg ttcgtcataa tttctgctga
    11701 aatcgacctt tccgtcggct caatgatggg gctattaggt ggcgtcgcgg cgatttgtga
    11761 cgtctggtta ggctggcctt tgccacttac catcattgtg acgttggttc tgggactgct
    11821 tctcggtgcc tggaacggat ggtgggtcgc gtaccgcaaa gtcccttcat ttattgtcac
    11881 cctcgcgggc atgttggcat ttcgcggcat actcattggc atcaccaacg gcacgactgt
    11941 atcccccacc agcgccgcga tgtcacaaat tgggcaaagc tatctccccg ccagtaccgg
    12001 cttcatcatt ggcgcgcttg gcttaatggc ttttgttggt tggcaatggc gcggaagaat
    12061 gcgccgtcag gctttgggtt tacagtctcc ggcctctacc gcagtagtcg gtcgccaggc
    12121 tttaaccgct atcatcgtat taggcgcaat ctggctgttg aatgattacc gtggcgttcc
    12181 cactcctgtt ctgctgctga cgttgctgtt actcggcgga atgtttatgg caacgcggac
    12241 ggcatttgga cgacgcattt atgccatcgg cggcaatctg gaagcagcac gtctctccgg
    12301 gattaacgtt gaacgcacca aacttgccgt gttcgcgatt aacggattaa tggtagccat
    12361 cgccggatta atccttagtt ctcgacttgg cgctggttca ccttctgcgg gaaatatcgc
    12421 cgaactggac gcaattgcag catgcgtgat tggcggcacc agcctggctg gcggtgtggg
    12481 aagcgttgcc ggagcagtaa tgggggcatt tatcatggct tcactggata acggcatgag
    12541 tatgatggat gtaccgacct tctggcagta tatcgttaaa ggtgcgattc tgttgctggc
    12601 agtatggatg gactccgcaa ccaaacgccg ttcttgattt tgataaaaat tttctcaaag
    12661 ccggttacgt attaccggtt ttgagttttt gcatgattca gcaggaaaag aaccatgttt
    12721 actaaacgtc accgcatcac attactgttc aatgccaata aagcctatga ccggcaggta
    12781 gtagaaggcg taggggaata tttacaggcg tcacaatcgg aatgggatat tttcattgaa
    12841 gaagatttcc gcgcccgcat tgataaaatc aaggactggt taggagatgg cgtcattgcc
    12901 gacttcgacg acaaacagat cgagcaagcg ctggctgatg tcgacgtccc cattgttggg
    12961 gttggcggct cgtatcacct tgcagaaagt tacccacccg ttcattacat tgccaccgat
    13021 aactatgcgc tggttgaaag cgcatttttg catttaaaag agaaaggcgt taaccgcttt
    13081 gctttttatg gtcttccgga atcaagcggc aaacgttggg ccactgagcg cgaatatgca
    13141 tttcgtcagc ttgtcgccga agaaaagtat cacggagtgg tttatcaggg gttagaaacc
    13201 gcgccagaga actggcaaca cgcgcaaaat cggctggcag actggctaca aacgctacca
    13261 ccgcaaaccg ggattattgc cgttactgac gcccgagcgc ggcatattct gcaagtatgt
    13321 gaacatctac atattcccgt accggaaaaa ttatgcgtga ttggcatcga taacgaagaa
    13381 ctgacccgct atctgtcgcg tgtcgccctt tcttcggtcg ctcagggcgc gcggcaaatg
    13441 ggctatcagg cggcaaaact gttgcatcga ttattagata aagaagaaat gccgctacag
    13501 cgaattttgg tcccaccagt tcgcgtcatt gaacggcgct caacagatta tcgctcgctg
    13561 accgatcccg ccgttattca ggccatgcat tacattcgta atcacgcctg taaagggatt
    13621 aaagtggatc aggtactgga tgcggtcggg atctcgcgct ccaatcttga gaagcgtttt
    13681 aaagaagagg tgggtgaaac catccatgcc atgattcatg ccgagaagct ggagaaagcg
    13741 cgcagtctgc tgatttcaac caccttgtcg atcaatgaga tatcgcaaat gtgcggttat
    13801 ccatcgctgc aatatttcta ctctgttttt aaaaaagcat atgacacgac gccaaaagag
    13861 tatcgcgatg taaatagcga ggtcatgttg tagcgatgat gagaattgtc ggcgtcacat
    13921 caggtaacgc tgcgtggttg tcggatgcgg cgtgaacgcc ttatccgacc tacccgccag
    13981 gcatgataaa acgcaccaac aacgcttcag gctcgtagct caactgcctg agacaaagta
    14041 aaaagcctta tccgactgac aagtcggata aggctcaagg aaatgcaatt acatatgcgc
    14101 cgcgattaac cgttggttat cctggtacat tgcgaacagg tagttgttat aactcttccc
    14161 tttggtcgag tagcccttca gcttgtgaat catcgctgtg gcagtcactt cctgatccgc
    14221 tttacgcagc tgcgcacgcg atttacggaa cgaagagtaa gccgggtgcg tattcaggtt
    14281 agtgacatag gcgctcaccg attctttgac agaactaaac tgtgagtacc ctttcacttt
    14341 acctggcgca ttggtacaac gtcctttcat gcatttcatg ccgaacaggt tgttgttgtt
    14401 gcgcgccagc ttcgacgttc cccaaccgct ttctgctgca gccatcgtcg ccaccatact
    14461 ggtggggata atgtctacgc gttcaagcaa ggtattccac gggatttttc gcgtattacc
    14521 ggaccacttc accttgtagc gtttggcgat gtctttcaga cgcgcacgct cagcaggtga
    14581 ccattggccc tgatactgtt ttgaaatgag ccagttacgt tccgcagtaa tggccgcatt
    14641 ttggctggta atgtaaggca ttacggtccg gagaaacgcc tttttccttg gtgttccgga
    14701 agggtatttt cgcaaatcag gaagtgaact actctttgca ctattgcgag aatactcttg
    14761 tttactgctt acctgtttat tactagcttt agttaagtgg gacttttgac tcgctgttgt
    14821 tgtgtgcgtc ttcgctaaca cctcactcga aaacaccaga gtgagtaaca taagaatcat
    14881 cgccccatat cgtcgtatgg gagtcaaaat catcaggtct cctggtcgga tttaatcatt
    14941 ccaacacctt atatttttca caaatttgag agttgaatct caaatcatat caaaaatagc
    15001 tgtcaagagc accccaagga atagtccaaa tctgaaacta tgtcacgtgt taacgattca
    15061 gattggcgct aaatcgcaga aaatgtgggg gttatcgcaa aattcagccg ttttttgcgc
    15121 gagatcgctc acccttgctt ctcatcctgt ggacttaccg ctcagggatg agttttgttt
    15181 ggcttatcgc tggcaaactg tctgaaatcg cagcaataag gactcatccg ccatgaaact
    15241 cgccgcctgt tttctgacac tccttcctgg cttcgccgtt gccgccagct ggacttctcc
    15301 ggggtttccc gcctttagcg aacaggggac aggaacattt gtcagccacg cgcagttgcc
    15361 caaaggtacg cgtccactaa cgctaaattt tgaccaacag tgctggcagc ctgcggatgc
    15421 gataaaactc aatcagatgc tttccctgca accttgtagc aacacgccgc ctcaatggcg
    15481 attgttcagg gacggcgaat atacgctgca aatagacacc cgctccggta cgccaacatt
    15541 gatgatttcc atccagaacg ccgccgaacc ggtagcaagc ctggtccgtg aatgcccgaa
    15601 atgggatgga ttaccgctca cagtggatgt cagcgccact ttcccggaag gagccgccgt
    15661 acgggattat tacagccagc aaattgcgat agtgaagaac ggtcaaataa tgttacaacc
    15721 cgctgccacc agcaacggtt tactcctgct ggaacgggca gaaactgaca catccgcccc
    15781 tttcgactgg cataacgcca cggtttactt tgtgctgaca gatcgtttcg aaaacggcga
    15841 tcccagtaat gaccagagtt acggacgtca taaagacggt atggcggaaa ttggcacttt
    15901 tcacggcggc gatttacgcg gcctgaccaa caaactggat tacctccagc agttgggcgt
    15961 taatgcttta tggataagcg ccccatttga gcaaattcac ggctgggtcg gcggcggtac
    16021 aaaaggcgat ttcccgcatt atgcctacca cggttattac acacaggact ggacgaatct
    16081 tgatgccaat atgggcaacg aagccgatct acggacgctg gttgatagcg cacatcagcg
    16141 cggtattcgt attctctttg atgtcgtgat gaaccacacc ggctatgcca cgctggcgga
    16201 tatgcaggag tatcagtttg gcgcgttata tctttctggt gacgaagtga aaaaatcgct
    16261 gggtgaacgc tggagcgact ggaaacctgc cgccgggcaa acctggcata gctttaacga
    16321 ttacattaat ttcagcgaca aaacaggctg ggataaatgg tggggaaaaa actggatcag
    16381 aacggatatc ggcgattacg acaatcctgg attcgacgat ctcactatgt cgctagcctt
    16441 tttgccggat atcaaaaccg aatcaactac cgcttctggt ctgccggtgt tctataaaaa
    16501 caaaatggat acccacgcca aagccattga cggctatacg ccgcgcgatt acttaaccca
    16561 ctggttaagt cagtgggtcc gcgactatgg gattgatggt tttcgggtcg ataccgccaa
    16621 acatgttgag ttgcccgcct ggcagcaact gaaaaccgaa gccagcgccg cgcttcgcga
    16681 atggaaaaaa gctaaccccg acaaagcatt agatgacaaa cctttctgga tgaccggtga
    16741 agcctggggc cacggcgtga tgcaaagtga ctactatcgc cacggcttcg atgcgatgat
    16801 caatttcgat tatcaggagc aggcggcgaa agcagtcgac tgtctggcgc agatggatac
    16861 gacctggcag caaatggcgg agaaattgca gggtttcaac gtgttgagct acctctcgtc
    16921 gcatgatacc cgcctgttcc gtgaaggggg cgacaaagca gcagagttat tactattagc
    16981 gccaggcgcg gtacaaatct tttatggtga tgaatcctcg cgtccgttcg gtcctacagg
    17041 ttctgatccg ctgcaaggta cacgttcgga tatgaactgg caggatgtta gcggtaaatc
    17101 tgccgccagc gtcgcgcact ggcagaaaat cagccagttc cgcgcccgcc atcccgcaat
    17161 tggcgcgggc aaacaaacga cacttttgct gaagcagggc tacggctttg ttcgtgagca
    17221 tggcgacgat aaagtgctgg tcgtctgggc agggcaacag taacttttcc ggcttcccgt
    17281 tcgtcagtac ctcgggaagc cgccaaccag gataaaatgt cagccctaat cagcgttgca
    17341 ggataaagca ccgctcactc ttcaacagac cgatttgcac cccagcaaat gtagcgttat
    17401 tgttaccttc cttgctacag agttcgacag atatcccgct atgacattct ccctttttgg
    17461 tgacaaattt acccgccact ccggcattac gctgttgatg gaagatctga acgacggttt
    17521 acgcacgcct ggcgcgatta tgctcggcgg cggtaatccg gcgcagatcc cggaaatgca
    17581 ggactacttc cagacgctac tgaccgacat gctggaaagt ggcaaagcga ctgatgcact
    17641 gtgtaactac gacggtccac aggggaaaac ggagctactc acactgcttg ccggaatgct
    17701 gcgcgagaag ttgggttggg atatcgaacc acagaatatt gcactaacaa acggcagcca
    17761 gagcgcgttt ttctacttat ttaacctgtt tgccggacgc cgtgccgatg gtcgggtcaa
    17821 aaaagtgctg ttcccgcttg caccggaata cattggctat gctgacgccg gactggaaga
    17881 agatctgttt gtctctgcgc gtccgaatat tgaactgctg ccggaaggcc agtttaaata
    17941 ccacgtcgat tttgagcatc tgcatattgg cgaagaaacc gggatgattt gcgtctcccg
    18001 gccgacgaat ccaacaggca atgtgattac tgacgaagag ttgctgaagc ttgacgcgct
    18061 ggcgaatcaa cacggcattc cgctggtgat tgataacgct tatggcgtcc cgttcccggg
    18121 tatcatcttc agtgaagcgc gcccgctatg gaatccgaat atcgtgctgt gcatgagtct
    18181 ttccaagctg ggtctacctg gctcccgctg cggcattatc atcgccaatg aaaaaatcat
    18241 caccgccatc accaatatga acggcattat cagcctggca cctggcggta ttggtccggc
    18301 gatgatgtgt gaaatgatta agcgtaacga tctgctgcgc ctgtctgaaa cagtcatcaa
    18361 accgttttac taccagcgtg ttcaggaaac tatcgccatc attcgccgct atttaccgga
    18421 aaatcgctgc ctgattcata aaccggaagg agccattttc ctctggctat ggtttaagga
    18481 tttgcccatt acgaccgagc agctctatca gcgcctgaaa gcacgcggcg tgctgatggt
    18541 gccggggcac aacttcttcc cagggctgga taaaccgtgg ccgcatacgc atcaatgtat
    18601 gcgcatgaac tacgtaccag agccggagaa aattgaggcg ggggtgaaga ttctggcgga
    18661 agagatagaa agagcctggg ctgaaagtca ctaaaaattg cctgatgcgc tacgcttatc
    18721 aggcctacat gatttctgca atatattgaa tttggaagaa tttgtaggcc ggatagggcg
    18781 tttacgccgc atctggcata aacaaagcac gctattccgg cgttgagaaa cgccggaaaa
    18841 cgtttcaaac aggctgctgc cgtagccgat gccgctgcac tttctcgaca tccatgcact
    18901 gcaacgcctt tgtcgggcag gcttcaacac aggccggtcc cgtctcccga tgccagcaca
    18961 gatcgcactt aatcgccctc gcctttttgc gcgaagaaac gacctccatc gcaccaaacg
    19021 ggcaagccag catacagctt ttacagccaa tgcaacgtgt ttgttcaacg aaaatatgcc
    19081 cttgttcgcg gcttatcgcg tcaacagggc agacattcgc gcacggtgca tcttcacact
    19141 gatgacaggc tactgccgtg gtccagctgt ggtctttaat gacacgaata cgggaaataa
    19201 actcgtctgg tgacaacgca gcgcaatcct gattctcctg atgcgacact gcgcaagcca
    19261 cttcacaggt acggcaaccg atacatttcg tcgcatccgc aataataaac cggttcatga
    19321 tcactccttt gcactaaaac agcagagtgc cagagagcgc gtccctactg ctttgatcca
    19381 acgaaggaaa tgttaaagtt ttggcacttg tccaaagcgt tatgtgatcg cgcccaggtc
    19441 atccctgaca gtaaatccca gttcattaga aatagcctgc gcggtttcac gcagtggttt
    19501 caggagattt ttctctccca cctgtttcag acgtgatgtc gaaagcgaaa tcgacacggc
    19561 gtacggcacc cgcccatgaa tatcaaacac cggaacagca atacaggaga cgccgagttc
    19621 gttttcttct ctgtccatcg ccgctccgct ttcacgaatg tgcgccagtt cgtcgaacat
    19681 cgcgggcagc tcggtaatgg tattgcgggt taacggctgg atctcatgct gatggctttc
    19741 ccagtatgac ttcacgtagt ccgggtgacc aaacgccatg tagatcttgc ccattgcgga
    19801 acagtagagc ggcatatgct ggccaatata ggcacgggtt cgcagcatcc cggttgtggg
    19861 ttccagctta taaatcaaaa tagcgtgatc gtcttcgcgg ctggagaagt taatggtttc
    19921 accagtggcg atgttcagtg cctcaagatg cggagcggcg atatgaatga tattcagcga
    19981 agacagcgcc ttctgcccga cggcaataaa tttggtggtc aggcgataac tccctgcggc
    20041 aggcgcggtg gtcacatagc cgcaggactg taatccctgc aataagcgat ggacggtact
    20101 cttatttaaa ccagccagct ccgaaagatg cgccaacgga caaccgtttg gatagttgct
    20161 caaaatctca atcagcatca acccgcgaaa cagactctgg cttccggctg gacgctcttt
    20221 ttcctgcgcc atctcgttct ctttttttcc catcacttct ttccccattt tgtcgcgtcc
    20281 tgatggtagc gcaaagtgtg ccgtagttca cgatctcgac agataattta taaccaattg
    20341 atttttatgt cttttgaaat tcatcaatca gattgctttg ttaaaaagtg atcgatatat
    20401 ttgaaatcaa gtttcgcata ttgaaatttt aagccaaaaa agcgatcaaa aaaacaagga
    20461 agcctgggat gaaagtgaca tttgagcagt taaaagcagc ctttaatcgg gtcttaattt
    20521 cacgcggcgt tgacagcgaa acggctgacg cctgtgcaga gatgttcgcc cgcaccaccg
    20581 aatccggcgt ttattctcac ggcgttaatc gtttccctcg tttcattcaa caactggaaa
    20641 acggcgatat cattcctgat gcccaaccca aacgtataac cagcctcggc gcaattgaac
    20701 agtgggacgc ccagcgttcg atcggtaacc tgacagcgaa aaagatgatg gatcgcgcca
    20761 ttgaactggc tgccgatcac ggtattggtc tggtggcact acgtaatgcc aaccactgga
    20821 tgcgcggcgg cagctacggc tggcaggcgg cggaaaaagg ctatattggc atttgctgga
    20881 ccaactccat cgccgtaatg ccgccgtggg gcgcaaaaga gtgtcgcatc ggcactaacc
    20941 cgctgatcgt cgccattcct tccacgccga tcaccatggt cgatatgtcg atgtcgatgt
    21001 tctcttacgg catgttagaa gttaaccgtc tggcaggtcg tcagctcccg gtcgatggtg
    21061 gctttgatga tgagggcaat ttgaccaaag aacctggcgt tatcgagaag aatcgccgca
    21121 ttttgccgat gggctactgg aaaggttctg gcatgtcgat tgtgctggat atgatcgcta
    21181 ctctcctttc cgacggcgca tccgttgccg aagtcaccca ggacaacagc gacgaatacg
    21241 gcatttcaca aatttttatt gccattgaag tggacaagct tatcgacggt cccacccgcg
    21301 atgccaagct gcaacgcatc atggattacg ttactagtgc cgagcgtgct gacgaaaatc
    21361 aggccattcg cttacccggc catgaattta ctaccctgct ggccgaaaac cgccgtaacg
    21421 gcatcactgt tgatgacagc gtgtgggcca aaatccaggc gttatgagga gataagtcat
    21481 gatttttgga catatcgcgc agcccaatcc gtgccgtttg cccgccgcca ttgaaaaggc
    21541 gctcgatttt ctgcgcgcta ccgatttcaa cgccctggag ccgggcgttg tcgaaatcga
    21601 cggcaaaaat atttatacgc aaattatcga tttaaccaca cgcgaagcgg tggtaaaccg
    21661 tccggaagtg catcgtcggt atatcgatat tcagtttctg gcgtggggcg aagagaaaat
    21721 cggcattgct attgatacgg gaaataataa agtcagcgaa tcactattag agcagcgcga
    21781 tattattttt tatcacgaca gtgaacatga atcatttata gaaatgatac cgggcagcta
    21841 cgcaattttc tttccgcagg atgttcaccg accaggttgt attatgcaaa cagcctctga
    21901 gatacgaaaa attgtggtta aagtcgcatt aacggcgctg aattaatcga ataatcgtct
    21961 acgccagaac gcctgggtaa tgttattgct ctaactatat taataattca ggttgctgca
    22021 catgcgactt gaattatgac agacatagcc tcaaggaata gctatgaaaa aaatactcga
    22081 agcaatactg gcgattaatc tcgccgtact ttcctgtatt gtatttataa acattatttt
    22141 aagatatggt tttcagacaa gcattttatc tgttgatgaa ttgtcacgtt atttatttgt
    22201 ctggttaacg tttattggcg cgattgtagc ttttatggat aacgcccacg ttcaggtcac
    22261 ctttctggtg gagaaactct cccccgcatg gcagcgacga gtcgctctgg ttacccattc
    22321 cttaatctta tttatttgtg gcgcactggc ctggggcgca acgctaaaaa caattcagga
    22381 ctggagcgat tattcaccga tcctcggttt acccatcggc ctgatgtatg ccgcttgtct
    22441 gcccaccagc ctcgttatcg cattctttga actgcgtcat ttatatcaac tcatcacgcg
    22501 tagcaattct ctaacgtcgc caccgcaagg agcttaatca tggctgtgct gatttttctg
    22561 ggctgtctgt tgggtggtat tgctatcggt ttgcctattg cctgggcact gttgttgtgc
    22621 ggggcggcgt taatgttctg gctggacatg tttgatgtcc agatcatggc acaaacgctg
    22681 gtgaacggag ccgatagctt ctccctgctg gcgattccgt tctttgtgct ggcgggtgaa
    22741 atcatgaatg cgggcggctt gtcaaaacgc attgttgacc tgccgatgaa actggtgggg
    22801 cataaaccag gcgggctggg ctacgtcggc gtgctggcag ctatgattat ggcgagcctt
    22861 tctggttctg ccgttgcgga taccgccgcc gttgccgctc tactggtgcc gatgatgcgc
    22921 agcgccaact atccggtcaa ccgggcggcg gggctgattg cctctggcgg cattatcgcg
    22981 ccaattattc ccccttccat tccgtttatt atcttcggcg tttccagcgg attatccatc
    23041 agtaagctgt ttatggcggg cattgcccct ggcatgatga tgggcgcaac gctgatgctt
    23101 acctggtggt ggcaggccag tcgccttaat ttaccccgcc agcaaaaagc aacgatgcag
    23161 gaaatctggc actcttttgt ctccggtatc tgggcgctgt ttcttccagt catcattatt
    23221 ggcgggtttc gctccggtct gtttacccct accgaagccg gggccgttgc tgctttctat
    23281 gcgctgtttg tcgccacagt tatttaccgt gaaatgacct tcgccacact ttggcatgta
    23341 ctcattggcg cggcgaaaac cacctcagtg gttatgtttc tggtggcctc ggcacaagtt
    23401 tccgcctggc tgattaccat tgctgaactg ccgatgatgg tttctgattt actgcaaccg
    23461 ttggtcgatt caccgcgtct gctgtttatc gtcattatgg tggctatcct gattgtcggc
    23521 atggtcatgg atttaacgcc gacggtatta attcttaccc cggtattaat gcctttagtt
    23581 aaagaggcag gaatcgatcc gatttatttc ggtgtcatgt ttatcattaa ctgctcaatc
    23641 ggcttaatta caccgcctat cggtaatgta cttaacgtta tttcaggggt ggcaaaactc
    23701 aaattcgatg atgcagtcag aggcgttttc ccttacgtcc tggttttata ttcattatta
    23761 gtcgtatttg tttttattcc cgatctcatc atcctccctt tgaaatggat taattaaaag
    23821 gaaaatatta tgaaattacg ctctgtaacc tacgcattat tcattgctgg cctggctgca
    23881 ttcagcacat cttctctggc ggcacaatct ttacgtttcg gttatgaaac atcacaaacc
    23941 gactcgcaac atattgcggc gaaaaaattc aatgatttat tgcaggagag aaccaaaggc
    24001 gagctgaaat taaaactgtt cccggacagc actctcggta acgcgcaggc gatgatcagc
    24061 ggcgtacgtg gcggcaccat cgatatggaa atgtccggct cgaataactt tgccgggtta
    24121 tcaccagtga tgaacttgct tgatgtccct ttcctgttcc gcgataccgc tcacgcgcat
    24181 aaaacgctcg acggcaaagt cggtgatgat ctgaaagcct cacttgaagg taaaggactg
    24241 aaagtactgg cctactggga aaacggctgg cgcgatgtca ccaactcgcg cgcaccggtt
    24301 aaaacccccg ccgacctgaa agggctgaaa atccgcacca acaatagccc gatgaatatc
    24361 gccgcattca aagtctttgg cgctaacccg atcccgatgc cgtttgccga agtctatacc
    24421 gggctggaaa cccgcactat cgacgctcag gaacacccga tcaacgtcgt ctggtcagca
    24481 aaatttttcg aagtgcagaa gttcctttct ctgacgcacc acgcctattc cccgcttctg
    24541 gtggtgatca acaaagcgaa gtttgatggc ttaagtccgg agttccagca ggcgctagtt
    24601 tcatctgcac aagaagcggg taactatcag cgcaaactgg ttgctgaaga tcagcaaaaa
    24661 atcatcgacg gcatgaaaga agcgggcgtg gaagtcatca ccgatctcga ccgcgaaggc
    24721 cgggaggcag gcgtgcagcg cctgccgctg tgcgcattaa gcccgcagcc aggctgggca
    24781 gagcgcgata tggcagaact gtggcaatgc tgcatggctg tcattcgcgc cctgcttact
    24841 cattctggtg ttagcgggga acaaattgtc ggtatcggca tctccgcaca gggaaagggc
    24901 ttgtttttgc tggataaaaa cgacaaaccg ctcgggaatg ctattttgtc ctcggaccgc
    24961 cgggcgatgg aaatcgttcg tcgctggcag gaagatggca tcccggaaaa actctacccg
    25021 ctgacccgac aaaccttgtg gaccgggcat ccggtgtcgc tgttacgctg gctgaaagag
    25081 cacgaaccag aacgctacgc gcaaattggc tgcgtgatga tgacgcacga ctacctgcgc
    25141 tggtgtttaa ctggcgtcaa aggctgtgaa gagagcaata tttccgagtc caacctctac
    25201 aacatgagtc ttggggaata tgacccgtgc ctcaccgact ggctggggat cgctgaaatc
    25261 aatcacgccc tgccgcctgt tgtcggatct gccgaaatct gcggggagat caccgctcag
    25321 acagccgccc tgaccggtct gaaagcgggt acgcccgttg ttggcggcct gtttgatgtg
    25381 gtttccaccg cactctgcgc cgggatcgaa gacgaattta ccctcaatgc ggtgatgggg
    25441 acctgggcgg tgaccagcgg cataacccgc ggtttacgtg acggtgaagc gcatccgtat
    25501 gtctatggtc gctacgttaa cgatggtgaa tttatcgttc acgaagccag ccctacctct
    25561 tccggcaacc tcgaatggtt taccgcacag tggggagaaa tctcgtttga tgagatcaat
    25621 caggccgttg ccagcttgcc gaaggctggg ggcgatctct ttttcctgcc gttcctgtac
    25681 ggcagcaacg ccggactcga gatgaccagt ggtttctacg ggatgcaggc cattcacacc
    25741 cgcgcgcacc tgttgcaggc catctatgaa ggggtggtgt tcagccatat gacccacctc
    25801 aaccgaatgc gcgaacgttt tactgatgtt cacaccctac gcgtcactgg cggcccggcg
    25861 cactccgatg tctggatgca aatgctggcg gacgtcagcg gtctgcgtat cgagctgccg
    25921 caggtggaag aaaccggctg ctttggtgcg gcccttgccg cccgcgtcgg caccggggtt
    25981 tatcacaact tcagcgaagc ccaacgtgac ttgcgacacc cggtgcgcac cctgctgcca
    26041 gatatgaccg cccatcagct ttaccaaaaa aaatatcaac gttatcagca tctcattgcc
    26101 gcacttcagg gctttcacgc ccgcattaag gagcacacat tatgagccga ccacttctgc
    26161 aactggccct cgaccactca tcacttgaag ccgcgcagcg cgacgtgacg ctgttaaaag
    26221 acagcgtcga tatcgtcgaa gcgggcacca ttctctgttt aaacgaaggg cttggcgcgg
    26281 tgaaagcctt gcgcgaacag tgcccggaca aaatcatcgt tgctgactgg aaggtcgccg
    26341 acgctggtga aacgctcgcg caacaggcgt ttggcgcagg cgctaactgg atgaccatca
    26401 tctgcgccgc gccgctcgcg acggtagaaa aaggccacgc aatggcacaa cgctgcgggg
    26461 gtgaaattca gatagagctg ttcggtaact ggacgctgga cgacgcccgc gactggcatc
    26521 gtattggcgt gcggcaggcc atttatcatc gcggtcgtga tgcacaggcc agcgggcaac
    26581 agtggggcga agccgatctg gcacgcatga aggcgctttc agatatcggc cttgagcttt
    26641 ccattactgg cgggattacc cctgctgacc tgccgctgtt taaagatatc cgcgtgaaag
    26701 cgtttattgc cgggcgggca ctggcaggcg cggcaaatcc ggcgcaagtg gctggcgatt
    26761 tccatgcgca aatcgacgct atctggggag gtgcgcgtgc gtaatcatcc gttagggatt
    26821 tatgaaaaag cgctggcgaa agatctctcc tggccggagc ggctggtgct ggcaaaaagc
    26881 tgcggttttg attttgtcga aatgtcggtg gatgaaaccg acgaacggct ctcacgtctt
    26941 gactggagcg ccgcacaaag gacttcgctg gttgccgcga tgatcgaaac aggtgttggg
    27001 atcccgtcga tgtgcctgtc cgcacatcgt cgctttccct ttggtagccg tgacgaggca
    27061 gtgcgcgaac gggcgcggga aatcatgagt aaagcgattc gcctggcgcg cgatctcggc
    27121 attcgcacca tccagctggc aggttatgac gtctattacg aagaccacga cgaaggcacc
    27181 cggcaacgtt ttgctgaagg gctggcgtgg gcagtcgaac aggcggcggc atcacaagta
    27241 atgctggcgg tggagattat ggataccgcg tttatgaact ccatcagcaa atggaaaaaa
    27301 tgggacgaga tgctcgcctc gccgtggttc accgtctacc cggacgtcgg caacctcagc
    27361 gcctggggca atgatgttcc cgccgaactg aaactgggca ttgaccgtat cgccgcgatc
    27421 cacctgaaag acacccagcc agtcaccggg caaagccccg gacagttccg tgatgtgccg
    27481 tttggcgaag gctgcgtcga tttcgttggc atcttcaaaa cgctgcatga actgaactat
    27541 cgcggttctt tcctgattga gatgtggacc gaaaaagcca aagagccggt gctggagatc
    27601 attcaggcgc ggcgctggat tgaagcgcgt atgcaggagg ctggatttat atgttagagc
    27661 aactgaaagc cgacgtgctg gcggcgaatc tggcgcttcc cgctcaccat ctggtgacgt
    27721 tcacctgggg caatgtcagc gcggtagacg acacgcggca atggatggta atcaaacctt
    27781 ccggcgtcga gtacgacgtg atgaccgccg acgatatggt ggtggttgag atagccagcg
    27841 gcaaggtggt ggaaggtagc aaaaagccct cgtccgatac gccaacgcat ctggcacttt
    27901 accgtcgcta tgccgaaatt ggtggcattg tgcataccca ctcgcgccac gccaccatct
    27961 ggtcacaggc cgggctggat ctccccgcct ggggcaccac ccacgccgat tatttttacg
    28021 gtgctatccc ctgcacgcga cagatgaccg cagaggagat taacggcgaa tatgaatatc
    28081 agaccggcga agtgatcatt gaaaccttcg aagaacgtgg caggagtccg gcacaaatcc
    28141 cggcggtgct ggtgcattct cacggcccgt tcgcatgggg taaaaacgcc gccgatgccg
    28201 tgcataacgc cgtagtactc gaagaatgcg cctatatggg tctattctcg cgccagcttg
    28261 cgccgcagct ccctgcgatg caaaacgaac tgctggataa gcactacctg cgtaagcatg
    28321 gggccaatgc ctattacggg cagtaatccc tcacgccggg gcttcatcgc cccggcacta
    28381 cgaattgata tgttccttgc tgtaacgccg cttccacgct gctggcgtta aaccagtatg
    28441 tttctgaaaa atctgccgga aatagccaac gtcattaaac ccgcacaacg cagccacttc
    28501 agtgagcgat cgcgcttcgc taattaacag cttttcggct tcgcgcactc gctggcgatg
    28561 gattgcttct gttaacgtca gatgaaacac ccgccggtag acgcgcccaa gataatcggc
    28621 attgcagtac agctcgcggg ccagaagcgc cgtagaaagc gggcgatgat attgcgtgcg
    28681 gatcagtttc tgcgctttcc aggcaagggt ggtgcctgcg ccatcggcct gttcatcaag
    28741 acgcgcgtca cgggtgagct gctgcaaaat cagcagcaca ataaactcca acgccggagt
    28801 gcgttgatgc ttttcctgtt cgcttaagaa ctggcgaaac agggccacca gcgcctgcgg
    28861 atcggcaacc ttcgtttgtt gcggcacgct aagatgagtg agccagcgcg ggtcgctatc
    28921 cggcgcttta acctcaaaat gcagccagta aaatttgaga tcggcgggaa aatcctccac
    28981 cccaacatgt tgtcgattcg gccagagtaa taaactctct cccgcatcga ccagaaatat
    29041 ccgttcttct tcctgaatcg ttaatcgacc tttttcgaca aagataattt cccatgactc
    29101 cagccgcctt gcaggatgcc tccctacgcc gcgggaaata aataatccac cattttgcac
    29161 tttaatcggt aacgtcatgg ataattcgag cattgatatg taatttcccg cttattcatt
    29221 aatgattaaa cacattacct tttgattatt aatcaaaaac attaatttca cctgatccat
    29281 ttcacacttt taaattcagg tcggaatcgt cacctttttt tactcttttc tcgccttgtt
    29341 gatattcccg ttcacggcag aattaatcag gtcatttcaa taaaactaaa aattgctccc
    29401 tattcacgag gaatatctat gacctctaca ccgattacta ccgctgatat cgctaacggt
    29461 aaaattgacg atgcgttatc tgtacgggaa aaaataggct acggcctggg tgacgcaggc
    29521 ggcaccgtaa taacttgcct gatcatgaat tttctcacct ttttctacac cgacgttttt
    29581 ggattaactc cggcgctggt tggcacgctg tttattgcac tgcgcgtgtt tgatgccatc
    29641 tccgacccgg tgatgggcgt cattgccgac cggacgcaaa gccgatgggg gcgctttcgt
    29701 ccgtggcagc tatggattgc cattcccatc ggcattatcg gcatcctgac gttcaccgtg
    29761 ccagatgcca gcatgggagt aaaaatcgcc tgggcgttcg gtacttacct gctcctttca
    29821 gtcggttata ccgccatcaa cgtaccgtac tgcgcgctga tcaacaccat gaccacccgc
    29881 cacaatgaag tgatctcctg ccagtcctgg cgattcgttc tctgcggcgt agcgggattt
    29941 ctggtttcgg taggcttacc gtggatggta gatctcttcg gtcagggcaa cgctgcacgc
    30001 ggctatcaac tgggcgtcgg cgtattgtgc gccattgccg tggtgatgtt cctgtgctgt
    30061 tttttctggg ttcgtgaacg ggtgccgctc tccacaatgg ggaaatttac cctgcgcgaa
    30121 catcttgccg ggctgcggaa caacgaccaa ctgctgctga tgctggtcat gtctttcctg
    30181 ctgattaacg tctttaatat tcgcggcggt gggtatatgt acttcattac ctacgtctta
    30241 caaggcagta cgggctacac gtcgctgttc ttcaccatgg tcaccttcgc ctccattatc
    30301 ggctcggtgg ttgtcagccc gttaacgcgg cgtttcgata ccgtcaaaat ttattactac
    30361 accaacctgc tcctcgctgc actggcggtg ttgatgtggt tcctgccctc cggcccggct
    30421 tatcaaacgc tgtggctggc ggtgatcctc ggtaatggcg tgattcttgg cttcacattg
    30481 ccattgcact tctcattgat ggcctttgcc gatgactacg gcgagtggaa aacccgcgta
    30541 cgttcttccg gcatgaactt cgccttcaat ctgtttttca tcaagctggc ctgggcctcc
    30601 agcgccggga tcatcagcct gctgtttatt tttgtcgcct accagcctgg cgtggaaaac
    30661 cagaccgcca gttcgcttgg cgggatcacg gcgatggaaa cattgctgcc tgcgctattc
    30721 cacctgctgc tggcaggggc gatccgcttt tgcaaactca ataatcctat gatgtcgcgc
    30781 atagctaccg acctgcgtca gcgtcatgta cagccttaag gagcacaaga tgaacatttc
    30841 ggaagtcgat ctgcgtaaac tgacggtcag cgatccgttc ctcggtcagt accaacaact
    30901 ggtccgcgac gtggtgattt cttatcagtg ggatgccttg aacgatcgta tcccagaagc
    30961 ggaacccagc catgcgattg aaaactttcg cattgccgcc ggacttcagg agggtgaatt
    31021 ttacgggatg gtgtttcagg acagcgacgt cgccaaatgg ctggaagcgg tagcctggtc
    31081 gctgtgccag aagccggacg ccgaactgga aaaaaccgcc gacgaggtaa tcgaactgat
    31141 cgcctacgcc caatgcgaag acggctatct caatacttac tttacggtaa aagcacccga
    31201 agaacgctgg agcaatctgg cggagtgtca tgaactttac tgcgcaggtc atctgattga
    31261 agccggagtc gccttcttcc aggccacggg aaaacgacgc ttgctggagg tggtttgccg
    31321 tctggccgat catatcgacc gcgtatttgg tccagatgaa gacaagctgc agggttatcc
    31381 cggtcatccg gaaattgaac tggcgctgat gcgtctctac gaagtcaccg aagagccgcg
    31441 ctacctggcg ctgacgaact attttgtcga acagcgtggt gcgcaaccgc actattacga
    31501 ccaggaatat gaaaagcgcg ggcagacctc gcactggcac acctacggcc cggcgtggat
    31561 ggtgaaagac aaagcctaca gccaagcaca tttgcccctt gcgcaacagc aaaccgccat
    31621 cggtcacgcg gtacgttttg cctacctgat gaccggcgtc gcgcatctcg cgcgtttaag
    31681 tcacgatgac agcaagcgtc aggactgcct gaggctgtgg aacaatatgg cccagcgtca
    31741 gttatatatt accggcggca ttggctcgca aagcagcggc gaagcgttca ctagcgatta
    31801 cgatctgccg aatgacacgg tttacgccga aagttgtgct tccatcggcc tgatgatgtt
    31861 cgcccggcga atgctggaaa tggaaggcga cagtcaatat gccgatgtga tggagcgcgc
    31921 gctgtacaac accgtgctcg gcggcatggc gctggatggc aaacatttct tctatgtgaa
    31981 tccgctggaa gtacatccaa aatcgctgaa attcaaccat atctacgatc acgttaaacc
    32041 gatccgccag cgttggtttg gctgcgcttg ttgtccgcca aatatcgccc gcgtgctgac
    32101 ctcgattggt cattatctct acacgccgcg tgaagatgcg ttgtatatca acatatatgc
    32161 aggaaacagc atggaagtgc cggtagaaaa tggcacgctg cgcctgcggg ttagcgggaa
    32221 ctatccgtgg caggagcagg tgacgattgc ggttgaatcg ccccagccgg tacgtcatac
    32281 gctggcttta cgtctgccgg actggtgcac acagccgcag atcatattga atggggaaga
    32341 ggtcgagcag gatattcgta aagggtattt gcacattacc cgcgaatggc aggagggcga
    32401 tacgctgaat ctgactttgc cgatgccggt acgccgcgtt tacggtaacc cgctggtgcg
    32461 tcacgtcgcc ggaaaagtgg cgattcagcg cggcccgctg gtgtattgcc tggaacaggc
    32521 cgacaatggc gagtcactgc ataatctgtg gctacccgcc gatgcgccat ttacgacatt
    32581 tgaaggtaat ggattgttcc gccataagat cttaatccag gcaccgggtt accggtatga
    32641 acagagcaat ccagagcagc aaccgctgtg gcattacgac agcgcgccag ccaaacgcca
    32701 gacgcaaact ctgaccttta tcccgtggtt tagctgggcc aaccggggtg aaggcgaaat
    32761 gcggatctgg gtgaatgagg aaaagcattg ccatccgtag gttggataag gcactcgcgc
    32821 cgcatccaac gtaaggagca cattgcctga tgcgacgctt aacacgtctt atcaggcata
    32881 caagtcggtg cgagagcgta ggccagccgc accgacatcc accggtgaat taaaaacgcc
    32941 agctcacgcc ggtgacaaag gtaaaactct cattacgatc aatcatcggg ctgtcggtaa
    33001 tctcttccgg cagctcgctg tatcctgcgc tcgccatcaa tacgacgtgc tctcctatcg
    33061 ggtattttgc cgtcaggctg acatagggca cccaggcatc ctgcgcggaa taacttgcca
    33121 gaccgctacg acgggagtca ctctctgaaa tgccatagta atagtcactg aaattctcgt
    33181 cataatagag aacgcccagc gcaggtgtca gcgacagacg acctatctgc attttgtgga
    33241 ataccgatag ctcccccacc cagccgttgc tgttatccag aacgtccgca gctgcagagg
    33301 ctttcacggt tccccaacgc tcgtggtgat accaggcaac ccccgccata gccgtactat
    33361 cacgcttatc aagctgttgc atggcataat cgtcattatc ggtagggtca aattccagcg
    33421 gcatccagga tgcggtcagg ctaagttcat ttttttcact ttgcgacaga ataaaaccta
    33481 acgtggtctg acgaacataa aaagtatcac cttcataact aatcagcgga attgccttcg
    33541 tattttcatt ataaccgcga taaggcgatt cattataagc cgcaccagca ccaatggata
    33601 attcagaagc agttgcgctt gccataaaag gcaacgcaaa taacgccaca atattgcgat
    33661 taa
//
LOCUS       DAJOSY010000044        33174 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJOSY010000044 DAJOSY010000000
VERSION     DAJOSY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.044"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..411)
                     /locus_tag="OHN27_002152"
     CDS             complement(<1..411)
                     /locus_tag="OHN27_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286591.1"
                     /translation="MKFRSLYKWDCPDTCKALVYFAQLLDEMLFDYTLDTYKPSVMNT
                     PTIGVETLNTIKDVEDGIIQPKNIEHLTAELIHNLSCDKVAQHLLGDAYQAFLNKLKN
                     TNISPKERSSIIEMLVIQLPPKLYKEKSEELITQE"
     gene            492..1070
                     /locus_tag="OHN27_002153"
     CDS             492..1070
                     /locus_tag="OHN27_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCQ2286592.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDRENWLCRVKTGDLETNWIS
                     WLTLRAGNARTWWRPSEGEQVVLLSLGGNLETAFALPAVYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEADDNITLKTSEFVLEADRTRINSEVVINGGVT
                     QGSGAMSSNGIVVDAHQHTGVLKGGDTTGGPV"
     gene            1067..1426
                     /locus_tag="OHN27_002154"
     CDS             1067..1426
                     /locus_tag="OHN27_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCQ2286593.1"
                     /translation="MTLYSGMNNTSGKAITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITINSNFDGSMVVGLTGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            1413..2321
                     /locus_tag="OHN27_002155"
     CDS             1413..2321
                     /locus_tag="OHN27_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HCQ2286594.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLAERKAEFVALHPKDEQEAVI
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAQAVMVAYAMGGDLDQLAANYNVKRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVEKALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPETEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            2314..2919
                     /locus_tag="OHN27_002156"
     CDS             2314..2919
                     /locus_tag="OHN27_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCQ2286595.1"
                     /translation="MNSLLPPGSTPLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGETPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWGG
                     AASYLGEEITIYPYINEIIISGGTAHEGGAVHVIDTMRVNP"
     gene            2916..3797
                     /locus_tag="OHN27_002157"
                     /pseudo
     CDS             2916..3797
                     /locus_tag="OHN27_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            3798..4553
                     /locus_tag="OHN27_002158"
                     /pseudo
     CDS             3798..4553
                     /locus_tag="OHN27_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            4644..4862
                     /locus_tag="OHN27_002159"
     CDS             4644..4862
                     /locus_tag="OHN27_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCQ2286596.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(5098..6783)
                     /locus_tag="OHN27_002160"
     CDS             complement(5098..6783)
                     /locus_tag="OHN27_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCQ2286597.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQHHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            7053..7430
                     /locus_tag="OHN27_002161"
     CDS             7053..7430
                     /locus_tag="OHN27_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCQ2286598.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(7460..7717)
                     /gene="grxA"
                     /locus_tag="OHN27_002162"
     CDS             complement(7460..7717)
                     /gene="grxA"
                     /locus_tag="OHN27_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCQ2286599.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            7877..8164
                     /locus_tag="OHN27_002163"
     CDS             7877..8164
                     /locus_tag="OHN27_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCQ2286600.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            8148..8870
                     /gene="nfsA"
                     /locus_tag="OHN27_002164"
     CDS             8148..8870
                     /gene="nfsA"
                     /locus_tag="OHN27_002164"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCQ2286601.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNSLTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            8931..9833
                     /gene="rimK"
                     /locus_tag="OHN27_002165"
     CDS             8931..9833
                     /gene="rimK"
                     /locus_tag="OHN27_002165"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCQ2286602.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            9921..10397
                     /locus_tag="OHN27_002166"
     CDS             9921..10397
                     /locus_tag="OHN27_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCQ2286603.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            10748..11860
                     /gene="potF"
                     /locus_tag="OHN27_002167"
     CDS             10748..11860
                     /gene="potF"
                     /locus_tag="OHN27_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCQ2286604.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRANLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            11955..13088
                     /gene="potG"
                     /locus_tag="OHN27_002168"
     CDS             11955..13088
                     /gene="potG"
                     /locus_tag="OHN27_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCQ2286605.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            13098..14051
                     /gene="potH"
                     /locus_tag="OHN27_002169"
     CDS             13098..14051
                     /gene="potH"
                     /locus_tag="OHN27_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCQ2286606.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFNVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            14048..14893
                     /gene="potI"
                     /locus_tag="OHN27_002170"
     CDS             14048..14893
                     /gene="potI"
                     /locus_tag="OHN27_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCQ2286607.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDSSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            14953..15441
                     /locus_tag="OHN27_002171"
     CDS             14953..15441
                     /locus_tag="OHN27_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCQ2286608.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            15482..16609
                     /gene="rlmC"
                     /locus_tag="OHN27_002172"
     CDS             15482..16609
                     /gene="rlmC"
                     /locus_tag="OHN27_002172"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCQ2286609.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVVEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(16808..17539)
                     /gene="artJ"
                     /locus_tag="OHN27_002173"
     CDS             complement(16808..17539)
                     /gene="artJ"
                     /locus_tag="OHN27_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCQ2286610.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKISDQWFPQ"
     gene            complement(17830..18498)
                     /gene="artM"
                     /locus_tag="OHN27_002174"
     CDS             complement(17830..18498)
                     /gene="artM"
                     /locus_tag="OHN27_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCQ2286611.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(18498..19214)
                     /gene="artQ"
                     /locus_tag="OHN27_002175"
     CDS             complement(18498..19214)
                     /gene="artQ"
                     /locus_tag="OHN27_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCQ2286612.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(19221..19952)
                     /gene="artJ"
                     /locus_tag="OHN27_002176"
     CDS             complement(19221..19952)
                     /gene="artJ"
                     /locus_tag="OHN27_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2286613.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(19970..20698)
                     /gene="artP"
                     /locus_tag="OHN27_002177"
     CDS             complement(19970..20698)
                     /gene="artP"
                     /locus_tag="OHN27_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCQ2286614.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(20916..21431)
                     /locus_tag="OHN27_002178"
     CDS             complement(20916..21431)
                     /locus_tag="OHN27_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2286615.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            21557..21880
                     /locus_tag="OHN27_002179"
     CDS             21557..21880
                     /locus_tag="OHN27_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCQ2286616.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            21877..22707
                     /gene="amiD"
                     /locus_tag="OHN27_002180"
     CDS             21877..22707
                     /gene="amiD"
                     /locus_tag="OHN27_002180"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCQ2286617.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(22704..23717)
                     /locus_tag="OHN27_002181"
     CDS             complement(22704..23717)
                     /locus_tag="OHN27_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCQ2286618.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(23816..25246)
                     /locus_tag="OHN27_002182"
     CDS             complement(23816..25246)
                     /locus_tag="OHN27_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCQ2286619.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(25257..26258)
                     /gene="ltaE"
                     /locus_tag="OHN27_002183"
     CDS             complement(25257..26258)
                     /gene="ltaE"
                     /locus_tag="OHN27_002183"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCQ2286620.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGIYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(26295..28013)
                     /gene="poxB"
                     /locus_tag="OHN27_002184"
     CDS             complement(26295..28013)
                     /gene="poxB"
                     /locus_tag="OHN27_002184"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCQ2286621.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(28146..29114)
                     /gene="hcr"
                     /locus_tag="OHN27_002185"
     CDS             complement(28146..29114)
                     /gene="hcr"
                     /locus_tag="OHN27_002185"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCQ2286622.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(29126..30778)
                     /gene="hcp"
                     /locus_tag="OHN27_002186"
                     /gene_synonym="priS"
     CDS             complement(29126..30778)
                     /gene="hcp"
                     /locus_tag="OHN27_002186"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCQ2286623.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(30922..31821)
                     /gene="lysO"
                     /locus_tag="OHN27_002187"
     CDS             complement(30922..31821)
                     /gene="lysO"
                     /locus_tag="OHN27_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCQ2286624.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(32278..32973)
                     /gene="aqpZ"
                     /locus_tag="OHN27_002188"
                     /pseudo
     CDS             complement(32278..32973)
                     /gene="aqpZ"
                     /locus_tag="OHN27_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
BASE COUNT         8054 a         8477 c         8808 g         7835 t
ORIGIN      
        1 ctcttgggtg ataagttctt cgcttttttc tttatataat ttgggtggta attgaattac
       61 cagcatttca attattgagc ttctctcttt agggcttatg ttagtatttt ttagtttatt
      121 gagaaaagct tggtaagcat cgcctaataa atgttgtgct actttatcac atgacaagtt
      181 atgtattaat tctgcggtta aatgttcaat atttttaggc tgaataatac catcctcaac
      241 atctttaatt gtatttaaag tttctactcc aattgttggg gtattcatca ctgagggttt
      301 gtatgtgtcc agtgtatagt caaatagcat ttcatccagt aattgcgcaa aatatactag
      361 tgctttacaa gtgtctgggc aatcccattt atacaaagaa cgaaatttca tttttgttat
      421 tccatattgt aagaccgctc atacaaatac atatgtttta agataaatgc aagatgttga
      481 atcattgccc tatgaacgca caacttaccg aaatcatgcg ccttatcacc aacctgatcc
      541 gcactggggt agtcaccgaa gtggaccggg agaattggct ttgccgggtg aaaacgggcg
      601 accttgaaac taactggatc agttggctga cgctgcgtgc cggtaatgcc cgcacatggt
      661 ggcgaccatc ggaaggtgag caggtggtgc tgctgagtct gggcggtaat ctggagactg
      721 cctttgcgct gcccgctgtc tattcgaatc agttcgcacc accgtcgacg tcggcggacg
      781 cctgcgtgac agaacatcct gacggtggct ggtttgaata cgaacccgcc accgggcgct
      841 ggtatgtcag gggcatcaaa tcaatggtca ttgaggccga tgacaatatc accctgaaaa
      901 ccagtgagtt tgtgctggag gctgaccgca cgcgtattaa cagcgaagta gtgatcaatg
      961 gtggcgttac ccagggcagc ggtgcaatga gttctaacgg aattgtggtt gatgcacatc
     1021 agcatactgg cgttctgaaa ggcggcgaca caaccggagg cccggtatga cgctttatag
     1081 cgggatgaac aataccagcg gtaaagccat tactgatatt gaccatctgc gccagtcggt
     1141 gcgggacatt ctgctgacgc cgcagggtag ccgcattgct cgccgtgaat atggttccct
     1201 gctgtcggca ctgatagacc agccacaaaa tccggcatta cgcctgcagg tcatgtcggc
     1261 agtgtatgtg gcgctgagtc gctgggagcc acggctgacg ctggattcca tcaccattaa
     1321 cagcaatttt gacggttcaa tggtggtggg gctgaccggg cggcgtaata acggtgtgcc
     1381 tgtttccctt tccgtatcaa caggagcaga gaatggcagt gattgacctt tcgcagttgc
     1441 ctgcgccgca gattgtcgat gtgccggact ttgagacgct gcttgccgaa cgcaaggccg
     1501 aatttgttgc gcttcatccg aaagatgagc aggaagccgt gatccgcacg ctggaactgg
     1561 aatctgaacc cgtcaccaaa ttgctgcagg agaacgctta ccgtgagttg cttctgcgcc
     1621 agcgcattaa cgaagccgcg caggctgtga tggtggctta cgcgatgggc ggcgatcttg
     1681 accagctcgc tgccaactac aacgtgaaac gcctgacggt gacgcctgct gataatgacg
     1741 ctgtgccacc cgttgcggct gtgatggaaa gcgatgaagc gttacgcctg cgtgtgcccg
     1801 cagcctttga agggctttct gttgcggggc caactgccgc ttatgaattt catgcccgaa
     1861 gcgccgacgg tcgggtggcg gatgccagtg caaccagtcc ggcacctgca gaggtggtgc
     1921 tgactgtcct gagccgcgaa ggcgatggaa cagcagaaaa agacctgctg gacgtggtgg
     1981 aaaaagctct gaacagtgag aacgtccgcc cggtggctga ccgtcttacg gttcgcagcg
     2041 cagaaatcat cccgtaccgg gtggaagcca ctatttttct ctatccggga ccggaaacag
     2101 agccggtaat ggcagcggca aaagccagcc tgcagaagta catcgccagt cagacgcgtc
     2161 ttggtcggga tattcgccgt agcgctatct ttgccgccct gcatgttgag ggtgtgcagc
     2221 gtgtggagct ggcttctcct ctggcggatg tggtcctgaa caaaacacag gcggcatcat
     2281 gtacgcagtg gagcgtaacc aacggaggaa cggatgaata gtctgctgcc accgggttca
     2341 acaccactgg agcgccgact ggcgcaaacc tgcagcggga tttctgatct gcaggtgccg
     2401 cttcgtgact tgtggaatcc ggcaacctgt ccggtcagtt tcctgcctta tctcgcctgg
     2461 gcgttctctg tagatcgctg ggacgagggc tggacagaaa gcgtcaagcg ccaggtggtg
     2521 aaggatgctt tttatattca tcagcataaa gggaccacca gtgccgtgcg tcgggtggtg
     2581 gagccgttcg gctttctgat ccgcattatt gagtggtggc agaccggaga gacaccgggc
     2641 acgtttcgtc tggatatcgg cgtgcaggac cagggcatca ctgaagatac ctatctggaa
     2701 cttgagcgac tgataagcga tgccaaacca tgtagccgtc acatgatcgg catgtccatc
     2761 aacctgcaga ccagcggccc gcattggggg ggagccgcca gctatcttgg cgaagaaatc
     2821 acgatctatc cgtatatcaa cgaaataatt atttccggtg gcaccgcgca tgaaggcggg
     2881 gcggtccatg ttattgacac aatgagagtg aatccatgag cacaaaattt tataccctgc
     2941 tgacggatat tggcgcggcg aaacttgcca gcgccgccgc gctcggtgtg cctttaaaaa
     3001 ttacccatat ggcggtcggc gatggcggcg gaacattgcc aacgccggac gcaaagcaga
     3061 cagcactagt aaatgagaaa cgccgggctg cgctgaatat gctctatatc gacccgcaga
     3121 acagcagcca gattattgcc gaacaggtga tccctgaaaa cgagggcggt tggtggatac
     3181 gtgaagtggg cctgtttgat gagtccgggg cattgattgc cgtgggcaac tgcccggaaa
     3241 gctataagcc gcaactggct gaaggcagcg ggcgcaccca gaccgtgcgc atggtgctga
     3301 ttaccagcag cacggacaat atcaccctga aaatcgaccc tgccgtcgtg ctggcaactc
     3361 gcaagtatgt ggatgacaaa atatcagagc acgaacagtc acgacgtcac ccggacgcct
     3421 cgctgaccgc aaaaggtttt actcagttaa gcagtgcgac caacagtgaa tccgaaatac
     3481 tggccgcaac accaaaggct gtgaaggctg catatgatct tgcagcaggt aaagcatccg
     3541 ccagtcacac acacccgtgg aatcagataa cggatgtgcc tgcagcttca ctgacggtaa
     3601 aaggcaccgt gcaactcagc agcgccacta acagcacgtc agaaacgcag gctgccacac
     3661 caaaggcagt gaaggcggca tatgaccttg cagcaggtaa ggcacctgtc agtcacacgc
     3721 acccgtggag ccagataacg gatgtgcctg cagcttcact gacggtaaaa ggcaccgtgc
     3781 aactcagcag cgccactacc cgccgtgaaa aatcgtggca taacaccacc atcggggaag
     3841 tggtgaagga aatagccgcg cggcacaagc tgaagatggc actgggtaaa gagctgtcgg
     3901 ataagcccgt ggagcatata gaccagacta atgagagtga cggcagtttt ctgatgcgac
     3961 tggcgcgaca gtacggtgcc atcgcgtcgg tgaaaaatgg caatctgtta ttcatccggc
     4021 aggggcaggg caaaagcgcc actggtaaac cactgccagt gatcactatc acacgcaagg
     4081 acggcgacag tcaccgattt accctggcag atcgcggagc ctacacgggc gtaattgcca
     4141 gctggttgca tacccgtgaa cccgcgaaga aagaaagcac cacggtgaag cgtaagcgca
     4201 gaactaagaa gcagaagaaa gagccggaag cgaagcaggg cgattacctg gtgggtacgg
     4261 atgaaaacgt gctggtactt aatcgcacct atgccagccg gggcaacgct gaacgggcag
     4321 cgaaaatgca gtgggaacgt ctgcaacgcg gcgttgcgtc attctcgcta caactggcgg
     4381 aagggcgggc agatctctac acggaaatgc ctgtgaaggt cagtggtttt aaacagccga
     4441 tagatgatgc ggaatggacc attacgactc tgacacatac cgtcagcccg gataacggtt
     4501 ttacgaccag tctggagctt gaagtgagga ttgatgattt cgaaatggaa tgattcttcg
     4561 caatggagaa cttttaagtt tgcaaaatgg aataatgcgg tatcattatt gtgaatttag
     4621 caaaaatggg gagaactcga aaaatgatga tttgcccact gtgtggaagt gccgcccata
     4681 ctcgcagcag ttttcaggta tcttcattga ccaaagagcg ttacaaccag tgccagaata
     4741 ttaactgcag ccatactttt gtaacccatg aaacttttgt tcgttcgatt gcaacgccaa
     4801 aagagtcaaa tccggttcag ccgcatccaa tgaaatcagg acaggtggcg ctctctcttt
     4861 gatgctgccg ccattttgtc gccatcgtta aaaaacagtg cttctaacgt catgatttta
     4921 aacggcataa atttcaggca acaaaaaacc catcaacctt gaaccgaaat ggcggggttg
     4981 atgggctcca caaaatgggg acatcaaaga aaagcagtgg cactaattaa gactgatgcc
     5041 ctgcggaaaa gttctgcggt tgtgcaaaaa aatttcattt tcagggcaac ttcagtttta
     5101 tcctaatcct ggccatacca tgacgatgat tgtccctgcc agcgtcagca ggacgttggc
     5161 gattgcgtag gtgcccgcat agcccagcgc agggatgtta ctgcgagctg tatcactgat
     5221 gatctccatt gccggcgcgc aggtgcgtgc gcccatcatt gcgccgaaca acagtgcgcg
     5281 gttcattcgc aatacataag caccgaacaa gaaacagata accacgggca caagactgac
     5341 aattaatccg gcaatcaaca tctgaccgcc aatcgcgccc aggccgttat taataccgct
     5401 accggcgctc agaccaacgc ctgccataaa caccatcaag ccgaactctt tcaccatgct
     5461 taatgcacct tgcggaatgt aaccgaaggt cgggtgatta gcacgcataa agcccagcat
     5521 aattccggcg aataacaacc cggcagcgtt ccccatgccg aaactgaatg tgctgaactg
     5581 gaaggtgatc atcccgatca tcagcccaat aacaaagaag gcgcaaaatg ccagcaggtc
     5641 agtgacctgg ctgtgaatcg agataaagcc gatgcgatcg gcgatggttt ttacgcgacg
     5701 ggcatcaccg ctgacttgta aaacgtcacc tttgttaagc acgacgttgt catctatcgg
     5761 catctcaatc tggctacgaa tgacgcggtt aaggaagcaa ccgtgatcgg tcaacttcag
     5821 ttgtgcgaga cgtttaccta cagcgttatg gtttttaacg accacttctt cagtgacgat
     5881 acgcatgtcg agaaggtcac gatcgaaaac ttctttaccg ttacggaagc tgggatcgag
     5941 tcgggcatgg gcgtcgggat agcctaccaa cgctatttca tcgcccattt gtagcacggc
     6001 atcaccgtct ggatttgcca gaatcccgtt acgtcgaata cgttcaatgt agcagccggt
     6061 ttgtcgataa atacccagtt cacgcagatt tttgccgtcg gtccaggcca ccagctccgg
     6121 gccgacgcga taggcgcgga tcaccggtaa ataaacctta cggttggcat cagtgtccag
     6181 gccacgttcg cgggcgattt gctgggcgct ggtctgtaag tcctgatgct gcaatttcgg
     6241 caagtaacgc gcaccaacaa tcaaactcac cagaccgatt aaataggtta aggcataccc
     6301 gaggctcaga ttatccagtg ccagtgagag ctgcctgctt tccatgccgg aatgacgcag
     6361 tgtatcgcca gcaccgacca gaaccggtgt cgacgtcata gagcctgcta acataccggc
     6421 cgtcaggcca atatcccagc caaacagctt acctaaccct aaggcgatca ccagcgcact
     6481 gccaaccatc accagtgcta acattaggta atttttccca tcgcgaaaaa aaatggaaaa
     6541 aaagttcggt ccggcttcaa ccccaacaca gaaaataaac agcataaagc caagattaag
     6601 cgcatcggtg ttaatgctga aatgatgttg gcctaataac agcgatacga ctaaaacgcc
     6661 aatggaatta cccagttgga tcgaaccaag tcgtaacttt ccgagacata gcccaagcgc
     6721 gaggaccaca aataataaca gaatgtaatt cccatttaac aattcggcga cgtttatatt
     6781 cacggaggct aacttcttgt ttactagtaa gctgttgaaa gaaatggtaa tttacgataa
     6841 tgttttttac cagaattcag ggcgcagatt cattcagcgc acctaaacga tagtaaagta
     6901 acaatatatt ttactagtgt aatcacatta ggtatcaacg gctatatgaa ttgcgttggc
     6961 ctatattagc atggaatgcg aagcggcttt atcttactga acgccacact ggcgaaaaat
     7021 gtgttcgata gacgcagtgt caggaggaac gagtgaaaca taaacaacgt tgggcggggg
     7081 caatctgctg ttttgtcctc ttcattgtgg tgtgcctttt tctggcgacg cacatgaaag
     7141 gcgcttttcg ggctgccggg catcctgaaa tcggcttgct gtttttcatt cttcctggag
     7201 cagttgccag tttcttttca cagcgtagag aagtcctgaa acctctgttt ggcgcaatgc
     7261 tggcggcacc ctgttcaatg cttattatgc ggctgttttt ttcaccgacc cgctcattct
     7321 ggcaagagct ggcatggtta ctaagcgcgg tgttctggtg tgcgctgggg gcactgtgtt
     7381 tcttatttat cagtagtttg tttaaaccac agcacagaaa aaatcagtaa agccctcaac
     7441 gtgagggctt gtcagacgat caggcgtcca gattttcttt cacccatgca gcaaaatcgg
     7501 tatagccgcc gatatgttgc tgatcgacaa aaatctgcgg cacggtttct acgggtttac
     7561 ctgccttttg ttgtagatct tctttagtga tcccttccgc acgaatatca acatactgat
     7621 actgaaaatc atcgcgttca ttgctcaatt tctcagccag atcttttgca cgcacacagt
     7681 aagggcaacc cggacgacca aaaataacgg tttgcattat ttctctcctc atagatttat
     7741 gcctgtaatg atcacgctaa aatgtattcg ctgaaagtag gtttaacctg ttgcattaat
     7801 tgctaaaagc tataactgtt aaacacaata cagtgaaaag ttttagactg aaggctcact
     7861 ttgcagaggg aagcgtatgc gcgcgatcgg taaattgcct aaaggcgtgt tgatactgga
     7921 atttatcgga atgatgctac tggcggtggc gctgctgtcg gtaagcgact ccctgtcgct
     7981 gcctgagcca ttttctcggc cagaagtgca gattctgatg atttttctcg gtgttttgct
     8041 catgcttccc gctgcggtgg tggttattct tcaggtggca aaacgtcttg ccccacagct
     8101 gatgaaccgt ccaccgcaat attcacgttc agaaagagaa aaagataatg acgccaacca
     8161 ttgaacttat ttgtggccat cgctccattc gccatttcac tgatgaaccc atttccgaag
     8221 cgcagcgtga ggcgattatt aacagcgccc gtgcgacgtc cagttccagt tttttgcagt
     8281 gtagtagcat tattcgcatt accgacaaag cattacgtga agagctggtg acgctgaccg
     8341 gcgggcaaaa acacgtagcg caagcggcgg agttctgggt gttctgtgcc gactttaacc
     8401 gccatttaca gatctgtccg gatgctcagc tcggcctggc ggaacagctg ttgctcggtg
     8461 tcgttgatac ggcaatgatg gcgcagaatt cattaaccgc agcggaatcg ctgggattgg
     8521 gcggcgtata tatcggcggc ctgcgcaata atattgaagc ggtgacgaaa ctgctgaaat
     8581 taccgcagca tgttctgccg ctgtttgggc tgtgccttgg ctggcctgcg gataatccgg
     8641 atcttaagcc gcgtttaccg tcctccattt tggtgcatga aaacagctat caaccgctgg
     8701 ataaagacgc actggcgcag tatgacgaac aactggctga atattacctc acccgtggca
     8761 gcaataatcg ccgggatacc tggagcgatc atatccgccg aacaatcatt aaagaaagcc
     8821 gcccatttat tctggattat ttgcacaaac agggttgggc gacgcgctaa aaccgccacg
     8881 tcgatgtatg atacgcgggc ttttgaccag gtctgacaga gaggtgcagg gtgaaaattg
     8941 ccatattgtc ccgggatgga acgctctatt cgtgtaagcg gctgcgtgaa gccgctatac
     9001 agcgcggtca cctggttgaa attcttgatc cgctttcttg ctacatgaac ataaatcctg
     9061 cggcgtcttc tattcactac aaaggccgca agttacccca ttttgacgca gtgatcccgc
     9121 gtattggcac cgccattacc ttttatggga cggcggcact gcgccagttc gagatgctgg
     9181 ggagctatcc gctcaatgag tcggtcgcca ttgcccgggc gcgtgacaaa ttgcgttcca
     9241 tgcaactgct ggcgcgtcag ggcatcgacc tgcctgtcac gggcattgcg cattcgccgg
     9301 atgataccag cgatttaatc gacatggtcg gtggtgcgcc gctggtggtc aagttggttg
     9361 aaggcacgca gggaattggc gtcgtgctgg cggagacgcg tcaggcggcg gaaagcgtga
     9421 ttgacgcttt ccgcggtctg aacgcgcata ttctggtgca ggaatatatc aaagaggcgc
     9481 aagggtgcga tatccgctgt ctggttgttg gcgatgaagt ggtcgctgcg attgaacggc
     9541 gggcgaaaga gggcgatttt cgttccaatt tgcatcgtgg cggcgcggca agcgtcgcca
     9601 gtatcacacc acaggagcgt gaaatcgcga taaaagccgc gcgaacgatg gcgctggacg
     9661 ttgctggtgt ggatattctg cgtgctaatc gcgggccgtt ggtgatggag gtgaatgcgt
     9721 cgccggggct ggaaggaata gaaaaaacca ccggtatcga catcgcgggt aaaatgatcc
     9781 gctggatcga acgccacgct acgacagaat attgcctgaa aacgggtggt tagtcgcaat
     9841 cacattactg atcatggttt tgcctgcgct ttttgcgtaa gctgtgccgg tctttttatc
     9901 gaaagaggtt gtacaaaatt atgacatcgc tggtcgttcc tggtctggat acgctgcgtc
     9961 aatggctcga tgacctgggg atgagttttt ttgaatgtga taactgtcag gctctgcatc
    10021 tgccccatat gcagaatttc gacggtgtct ttgatgccaa aatcgatctg atcgataaca
    10081 cgatcctgtt ttctgccatg gcggaagtcc gaccttcagc cgtattgccg ctggcggcgg
    10141 atttatctgc catcaatgcc agttcgctga ccgtgaaagc atttcttgat atgcaggatg
    10201 ataatctgcc aaagctggtg gtttgccagt ctttatccgt tatgcagggc gtaacctatg
    10261 agcagtttgc atggttcgtg cgtcagagcg aagagcagat ttcgatggtc attcttgaag
    10321 ctaatgccca tcaactgctg ttaccgactg atgatgaagg gcaaaacaac gttaccgaaa
    10381 actatttcct ccactgataa ctcctttcga gcacgcagtc gctggtgcag tggctgcgcg
    10441 ctgcaaaatt atctgctgtt tttaaccttt tcttaaagat tatttcactt ctcttgtgtc
    10501 gatttggctt tatcacatag agcaaatatg cataaaaatt tgttaaatac cgttttttaa
    10561 tccgagctat agtctcaaac cctggctaaa gttattcttg cgatgctttt atatagcgag
    10621 cagtgctggc cgggagaaag ttctcttttc ttacaccgcg ccgataaaaa atatgcacgt
    10681 ttattgcata tctttcagtg tgacaacttt tgttcgtttg ttaacgaact ttcagaagga
    10741 aagagatatg accgccttaa ataaaaaatg gctatcgggt ctggttgcgg gtgctctgat
    10801 ggccgtctct gtcggcacgc tcgcggctga acaaaaaaca ctccacattt ataactggtc
    10861 tgattatatc gccccggaca cggtggccaa ttttgaaaaa gaaaccggta ttaaagtcgt
    10921 ctacgatgtt ttcgactcta acgaagtact ggaaggcaaa ttaatggccg ggagtaccgg
    10981 ctttgatctg gtggttccat ctgccagctt tctggagcgc cagttgactg cgggagtttt
    11041 ccagccgctg gacaaaagca aattgccgga gtggaagaat ctcgacccgg aactgctgaa
    11101 gctggtcgcc aaacacgatc ccgacaataa atttgctatg ccctatatgt gggcgacgac
    11161 cgggattggc tataacgttg ataaagttaa agcggtgctg ggcgaaaacg cgcccgtcga
    11221 tagctgggac ttgatcctca aacctgaaaa tctggaaaaa ctgaaaagct gcggtgtctc
    11281 tttcctggat gcgccagaag aagtttttgc taccgtgttg aattatctcg gcaaagatcc
    11341 caacagcact aaagcggatg attacaccgg accggcaaca gatctgctgt taaagctgcg
    11401 cccgaacatt cgttatttcc attcatctca atacattaac gacctggcaa acggcgatat
    11461 ttgcgtcgct atcggctggg caggtgatgt ctggcaggcg tcaaaccgcg cgaaggaagc
    11521 gaagaatgga gtgaatgtct cgttctcgat tccaaaagaa ggggcgatgg cgttctttga
    11581 tgtattcgcc atgcctgcgg atgccaaaaa caaagacgaa gcctatcagt tcctgaatta
    11641 cctgctgcgc ccggatgtag tagcgcatat ttccgaccat gtgttctatg ccaacgccaa
    11701 taaagcagcc acgccgctgg tgagtgcgga agtccgtgag aacccgggta tttatccgcc
    11761 tgcggatgtt cgtgcgaatc tgttcactct gaaagtgcag gacccgaaaa tcgaccgtgt
    11821 gcgcacccgc gcgtggacca aagtgaagag cggaaaataa tccgcagcct tagatgccgg
    11881 gcgggcgcac cacacccgcc ggcaattcgc accatcatgg tgcgcttgca cacattcaat
    11941 gccggagagc agccgtgaat gacgctatcc ctcgcccgca ggcgaaaacc cgtaaggcgc
    12001 tgacgccgct attagaaatc cgcaacctca ccaaatccta cgatggtcaa catgcggtgg
    12061 atgatgtcag cctgactatc tacaaaggtg aaatcttcgc gctgctgggc gcttccggct
    12121 gtggtaaatc cacgctgctg cgtatgctgg caggttttga acaaccctct gccggacaga
    12181 ttatgcttga tggcgtcgat ttgtcacagg tgccacctta tctacgcccc atcaatatga
    12241 tgtttcagtc ttacgcattg tttccccata tgactgtgga gcagaacatc gcctttggcc
    12301 tgaaacagga caaactaccg aaagcggaaa ttgccagccg ggtcaacgag atgcttgggc
    12361 tggtgcatat gcaggagttc gccaaacgca aaccgcatca gctttccggc ggtcagagac
    12421 agcgtgtggc gctggcccga agccttgcca agcgcccaaa acttctgctg ctcgacgaac
    12481 cgatgggcgc gctggataaa aaattgcgtg accggatgca gcttgaagtg gtggatattc
    12541 tggagcgcgt cggtgtgacc tgcgtgatgg ttactcacga tcaggaagag gcgatgacca
    12601 tggcggggcg catcgccatt atgaatcgtg ggaaatttgt ccagattggc gaaccggaag
    12661 agatctacga gcatccgact acccgctata gcgccgaatt tattggctcg gtaaacgtct
    12721 ttgaaggcgt actcaaagag cgtcaggaag atggcctggt gcttgattcg ccggggctgg
    12781 tgcatccact gaaagtcgac gcggatgcct cggtggtcga taacgtgccg gtacatgtgg
    12841 ctctgcgccc ggaaaaaatc atgctttgcg aagagccgcc cgccaatggt tgtaacttcg
    12901 cggtggggga ggtgatacat attgcctatc tcggcgatct ttcggtgtat cacgttcgtc
    12961 tgaaaagtgg gcagatgatt agcgcccagc tacaaaacgc ccatcgtcat cgtaaagggt
    13021 taccgacctg gggcgacgaa gtgcgtttgt gctgggaagt ggacagctgt gtggtgctga
    13081 cggtttaagg agcaaagatg aatacacttg aacctgctgc ccagtcgaaa ccgccgggcg
    13141 gatttaagct gtggttgtcg cagctgcaaa tgaagcatgg gcgcaaactg gtcattgcgt
    13201 tgccatatat ctggttgatc ttgctgtttc tgctgccatt tctgatcgtc tttaaaataa
    13261 gtctggcgga gatggcgcgc gccattccgc cttataccga actgatggag tgggctgacg
    13321 ggcaactgtc catcactctt aatctcggta atttcctgca actgaccgac gatccgctct
    13381 atttcgatgc ttatctccag tcgctacagg tggctgcgat ttcgacattt tgctgtttac
    13441 tgatcggcta tccgctggcg tgggcagtgg cgcacagtaa gccttcgacc cgtaatatct
    13501 tattactact ggtgatcctg ccgtcgtgga cctcgtttct gattcgtgtc tacgcctgga
    13561 tgggaatatt aaaaaacaac ggtgtgctga ataattttct gctgtggctg ggggttatcg
    13621 atcaaccgct gaccattctg cataccaatc tggccgttta tatcggcatt gtttacgctt
    13681 acgtgccgtt tatggtactg ccgatttata ccgcgttgat tcgtattgat tattcgctgg
    13741 tggaagcagc gctggatctc ggtgcacgac cgctgaaaac gttctttaat gtgatcgtgc
    13801 cgctgactaa aggtgggatt attgccggat cgatgctggt gtttatcccg gctgtgggcg
    13861 agtttgtgat cccggaactg ctcggtggcc cggacagcat catgatcggg cgcgtgctgt
    13921 ggcaggagtt ctttaacaac cgcgactggc cggtggcctc ggcggtagcg atcatcatgt
    13981 tgctgctgct aattgtgccg ataatgtggt ttcacaaaca ccagcaaaaa agcgtgggag
    14041 aacacggatg aataatttac cggtagttcg ttcgccctgg cggattgtga ttttgctgtt
    14101 gggcttcacc tttctctacg cgccaatgct gatgctggtg atctattcat tcaacagctc
    14161 gaaactggtg acggtgtggg ccggctggtc aacgcgctgg tatggtgagt tattgcgcga
    14221 tgacgcgatg atgagtgcgg ttggtttaag cctgacaatt gctgcctgtg cggcaacggc
    14281 ggcggcgatc ctcgggacta ttgcggcggt ggtgctggtg cgctttggca ggtttcgcgg
    14341 atcaaatggc tttgccttta tgatcaccgc gccgctggtg atgccagatg tcatcacggg
    14401 cttgtcgctg ttgttgttat tcgtcgcgct tgctcatgcc attggctggc ctgcggaccg
    14461 cgggatgctt accatctggc tggcgcatgt cacgttctgt acggcttatg tggcagtcgt
    14521 tatttcgtcg cgtctgcggg aactggatag ctcgatagaa gaagcagcga tggatctcgg
    14581 tgcgacgccg ctgaaagtat ttttcgtcat tacgctaccg atgatcatgc ccgcgatcat
    14641 ttctggctgg ttactggctt ttactttgtc gcttgatgat ctggtgatcg ccagctttgt
    14701 ttctgggccg ggagccacca cgttaccgat gctggtcttt tccagcgtgc ggatgggggt
    14761 gaatccggaa atcaacgccc ttgcaacgtt aattctcggt gcggtcggaa ttgtcggatt
    14821 tatcgcctgg tatctgatgg ctcgcgcaga aaaacagcgg atacgcgata tccagcgtgc
    14881 aagacgtggc tgaagacact aaaatttgcc aacctggcta cataatgccg cgcatgccgc
    14941 ggcattgttt tcatggaaga cgaaacgttg ggatttttta agaaaacatc ttcatctcat
    15001 gctcgcctga atgtgcctgc gctggtgcag gtagcggcgc tcgccattat tatgatccgt
    15061 ggcctcgacg tgctgatgat tttcaatacg ctgggcgtgc gcggtattgg cgagttcatt
    15121 catcgcagcg tacaaacctg gagcttaacg ctggtctttt taagcagtct ggtgctggtt
    15181 tttatcgaga tctggtgtgc gttttcactg gtgaaagggc gtcgctgggc gcgctggcta
    15241 tatctgctga cacaaatcac cgccgcaagt tacttgtggg cggcttcgct ggggtatggt
    15301 tatccggagc tgttcagcat tcccggtgaa tcaaaacgtg aaatcttcca tagcctgatg
    15361 ctgcagaagc tgccggatat gctcatcctg atgctgctgt tcgttccctc gaccagtcgg
    15421 cggttcttcc agttgcaata atgtgtataa tcgtcggccc tgatgatgtg aaggtcaatg
    15481 tatgcagtgc gcactttacg acgcgggtcg ctgtcgttcc tgtcagtgga taacgcagcc
    15541 gattccagag caactctccg ctaaaaccgc cgatcttaaa aatctgctcg ccgattttcc
    15601 ggtcgtggaa tggtgcgcgc cggtgtcagg cccggaacaa gggtttcgta ataaagccaa
    15661 aatggtggtg agtggtagcg ttgaaaaacc actgctcggt atgctgcatc gcgacggcac
    15721 accggaagac ctttgtgact gcccgcttta tcctgcctca tttgcgcccg tttttgcggc
    15781 gctaaaaccg tttatcgccc gagcggggct aacgccttat aacgtggcgc gtaagcgtgg
    15841 cgaactgaaa tacattctgc tgactgaaag ccagagtgat ggcggcatga tgctgcgctt
    15901 tgtactgcgt tctgatacca agctggcgca actgcgtaag gcgctgccgt ggttacacga
    15961 acaactaccg cagctgaaag ttattaccgt caatattcag ccggtacata tggcgattat
    16021 ggaaggggag acggagatct acctgaccga acaacaggca ctggcggagc gttttaatga
    16081 cgtaccgctg tggatccgtc cgcaaagttt cttccagact aatccggcgg tcgccagcca
    16141 gttgtacgcc accgcgcgcg actgggtacg acagctgccg gttaaacata tgtgggatct
    16201 gttctgcggt gtggggggct ttggtttaca ctgcgcgacg cctgacatgc agttaaccgg
    16261 gatcgaaatt gcatcagagg ccattgcctg tgcaaagcag tcagccgctg aactgggctt
    16321 aacgcgtttg caatttcagg cgctggactc cactcagttt gccaccgctc agggggatgt
    16381 gccggagctg gtgctggtta acccgccgcg ccgcggcatt ggtaaaccgc tgtgtgatta
    16441 tctctcaacg atggcaccgc gttttatcat ctactccagc tgtaacgccc aaactatggc
    16501 gaaagatatc cgcgaactgc ctgggtttcg tattgaacgg gtgcagcttt tcgatatgtt
    16561 cccgcatacc gcgcactatg aagtgctgac gctgctggtg aagcaataaa aaagccgcat
    16621 gtgcggcttc agattgctga caaagtgcgc gttgtttatg ccggatgcgg cgtaaacgcc
    16681 ttatccggcc tacaaaagcg tgcaaattca atacattgca tgggccatgt aggcctgata
    16741 agcgtagcgc atcaggcaat tttacctttg tcatcagtct caagccgcgg ttgcggcttt
    16801 ctgaatctta ctgtgggaac cactggtcac tgattttttg ataagtaccg tcagctttaa
    16861 ttgctgccag cgcgttattc agtttttcca gcagggcttt gttatccgga cgtacagcga
    16921 tgcccaggcc ggtgccaaaa tattgcggat cggtcacttt ctcagtagca acaccaagtt
    16981 gtggattggt tttcagccat tcgtttacca ccgctgtgtc accaaatacc ccatcaatac
    17041 gaccattttt cagatcgata aaggcattct gataactgtc ataagagaca gttttcactt
    17101 ccgggtgctg atcctgaata tatttctggt gcgtagtacc gttttccatc ccaatacgtt
    17161 tgcctttcag atcggcaaac gttttgtagg tatctttttt ggcaatcacg acggctgagt
    17221 tttcatagta gggcgtggta aacgatacct gtttgctacg ctccggggtg atatccatac
    17281 cggagattac ggcgtcatat tttctgaatt tcagggacgg gatcaggctg tcgaacgcgt
    17341 gattagtaaa agtacattct gcctgcattt gtttgcacaa ggcttttgcc agatcgatat
    17401 caaagccgac aatctcatta ttagcaccta tagattcaaa gggtggatag gtggctgaaa
    17461 cgccaaaatt gattttctct gcggcagaag caccgaaagt aaaggaagca agtaaagcgg
    17521 caagaactaa ctttttcatg atggaactcc cgtctgtcaa tcttatgatt tttggccgtg
    17581 tctgcggcat gggataacaa tgccatcaag tgaatttata tgcaataaac atgattaaat
    17641 aatttaaatg aaataaaaaa gacggacaac ttagtgggtt gtccgtcttc attataagaa
    17701 tttatgcact atgtagaccg gataaggcgt ccccgccgca tccggcacag gcaccgtgct
    17761 gatgtctgat gcgacgctgg cgcgtcttat cagacctaca aaaccccccg gcgaatgtac
    17821 gcagccacat taatttcgcc gttcgaatgc cagcgctttg cgctcgatca gacgcatcat
    17881 cagcgtcagc aggccgttaa cgaccaggta aataatccct gccgcaccga acaccattac
    17941 atcgtaggtg cgtccgtaca acaactggct gtatcccatc acttccatca gcgtaatggt
    18001 gtatgccaga gaggtacttt tgaataccag caccacttcg ttggaataag aagagagcga
    18061 gcgtttaaag gcatacggca gcaggatcgc cagcgtatct tttttgctca ttcccagggc
    18121 gctacaggac tgccactgac cttccgggat cgcacgaatt gcaccgtaaa acagctgcgt
    18181 ggtatacgcc gcactattca gcgacaacgc aatcagcgca cataaccacg gttctgacaa
    18241 caaatgccac agtgccggat actcctgcaa agtcggaaac tggcccggcc cgtaataaat
    18301 caggaagatc tgcaccagca gcggcgtacc ggtaaacagc gtgatataac cccgcaccag
    18361 ccacaccagc accggcgttt tcagcgtcag gatgatggta aaaatcaatg ccagaatcag
    18421 tgccacaatc agcgaggcaa cggttagcgt caggctggtg tgtagccctt tcatcagttc
    18481 gggtaaatac tcaaacatta gctgggcctc cgctcaaaac gtgtcgcgcg caggtcaatg
    18541 cgtttgagaa tgtactgact gagcagggtg atcaccaggt aaatcgccgc cgccacaatg
    18601 taccaggtaa atggttcctg ggtacgagta gcgatgcttt ttgtttgcag cattaaatca
    18661 ttcacactaa tcaaactgac cagcgcggta tctttcagca gcaccagcca ctggttaccg
    18721 aggccaggca gcgcatgacg ccacatctgc ggcatcacca gacggaaaaa gatagccgat
    18781 ttcgacagcc ccagcgcctg accagactcc cactgaccca ccggcaccgc tttcaacgcg
    18841 ccccgaagcg tttgcgaggc ataggcggca tacagcagtg acagagcgat gacaccacaa
    18901 aggaacgggc taacgtcgaa gttctcaatg tccatctgca ctgggatctg cacgaaccca
    18961 agattgatag tgaagccatc cgaaagcgtc agcagcagct gcgaggagcc aaaatagata
    19021 aacagcacca ccagaatttc tggcaggcca cgcagaatgg ttaccagcgc tgaacctgcc
    19081 cacgcgacag gacgccattt tgccgactcc cataccgcaa agaacatcgc cagcgccagc
    19141 ccgacaatca atgcacaaac ggcaaggccg acggtcatcc cggcggcgct tgctaaagga
    19201 aaaaattcat tcatcaggaa ttacttctgg aaccatttgt tgtagatggt ttcgtaagtg
    19261 ccatctttct tcactttttc cagcgcagtg ttgagtttct gctgcagctc agtgttgccc
    19321 tgacgtaccg cgatgccgag gccagtgccg aagtaatctt tatcggtcac tttgtcgccc
    19381 accgccgcca gtttcgggtt atctttcagc cactcagtga ccactgcggt gtcaccgaag
    19441 acgccgtcga tacgcccgtt ttgcagatcc agttttgcgt tctggtagct gtcatacgga
    19501 acggtagtga tttccgggtg cttatccata atgaatttct ggtgtgtcgt cccgttctgt
    19561 acgccgactt ttttgccttt cagctgatca acactggtgt atttgccttg ctgacccaca
    19621 aacagggcag agttgtcata gtacggggtg gtaaacagca cctgcttttc acgctccgga
    19681 gtgatatcca tgcccgccat cacggcttct acgcgacgga atttcaggct tgggatcagg
    19741 ctgtcaaacg cctggttaga gaaagtgcag gttgcatcaa tctctttaca cagcgcttgt
    19801 gccaggtcga cgtcaaaacc aacgatctgg ttgtttgcat caatcgattc aaacggagga
    19861 taggaggctt cggtagcaaa acgaatggtt tcggcagctg tggcggaaag actaaaacct
    19921 gcaattaacg cggcaatcag aacttttttc attgttgtta tcccgaatct tagtgagaga
    19981 gatagttttt aaatgcttcg gtttgcggct cggtaaagca gctcgcgtcg ccttgttcta
    20041 cgatatgacc attttccata tacaccactc ggctggcggt tttacgcgcc acttcaactt
    20101 cgtgggtgac gatcacctgg gtaatattcg tttctgccag ctcacgaatg atgctgacga
    20161 tttgtgccgt aatttccggg tccagtgcgg cggtcggttc atcgaacagc agtacctgcg
    20221 gttccatcat caacgcacgg gcaatagcaa cacgctgctg ctgaccacca gaaagatgca
    20281 gcgggtaacg atcgctataa ggtttgagac gcagacgttc cagcagtttt tctgcacggg
    20341 ccagcgcctg atctttactc aaccccagta cacggcaggg cgcttcaatc aggttttgct
    20401 gcacggtcag atgcggccac aggttgtatt gctgaaacac catgccaacg ttacgacgca
    20461 aatcgcgaat cgctttgtca gagggtgttt tggtgaaatc gaaatggttg cctgcaatgt
    20521 tgagcgtacc ggagcgcggc atctcaagca gattgagtac acgcagcagc gagcttttac
    20581 ccgcgccgct ggggccaagt aacaccagcg tttcgccctg tgggcaatcc agcgtgatat
    20641 cgaacagcgc ctgatgcgcg ccgtagaagc aattaatgcc gtttaattga atactcattg
    20701 acactcgtat actggcagtc tgatagctat tgaggtcgaa gatagtacct ttgacagaat
    20761 aattatgcaa tatttctgct ttaaaagtta aaagcaaagc gcattattca ataaacatag
    20821 cacaaaataa cgggggcggt ggtcggcgag cataaatgtc ggcattcctc acgaaatgcc
    20881 ggacaattta cggggtttat tggttgatca aggcgttagc gattctcgat ggactgacgg
    20941 agcgtacccg ccgtggcatg aacgctaccg cctaagtaac gcacatcgtc gatgacccaa
    21001 cactggcctt cctgaatcat taacacttca tcctgccaac cctggtcacc ctgtttgaga
    21061 tccacgcgca atggaatgtt acgggcatca cgattaggga tagtcgatgc actggcaacg
    21121 tgggcgctat ctggcaaggt ggttcgactg gagaatggat cgttggtcag tagttcccga
    21181 tggttattat cccgggaggc atcgctaagc agtgtcgcca gtttgtcgct cagataaggg
    21241 cgcaaggcgg tgatgtcgtt gctgcggtgc aaaatgcggt agtcataaaa ttgctgggcc
    21301 acgttatccg ggcctccttc aacgcaagga ccactgcgtg tgccgttatc tttataagct
    21361 ggagtgactg tggtgcaggc actgaggagc agtgcgcagg ggataagcat tgtcaatttg
    21421 ctgtagcgca taatgatttc cttataagcg atcgctctga aagcgttcta cgataataat
    21481 gatatccttt caataatagc gtatcagtct gataatgctt ttgagatcga aggcttagca
    21541 aacaaggaga tcgatcatgc aattttctac aaccccaact ctggaaggcc agaccatcgt
    21601 tgaatattgc ggtgtggtga ccggcgaagc gattttaggt gccaatattt tccgtgattt
    21661 ctttgccggt atccgcgata tcgttggcgg acgttccggt gcgtatgaaa aagaactgcg
    21721 taaagcacgg gagatcgcct ttgaggaatt aggctcccag gcgcgggcgc tgggggccga
    21781 tgccgtcgtc ggtattgata tcgactacga aacggtcggg caaaacggca gtatgctgat
    21841 ggttagcgtc agcggtacgg cggtgaaaac gcgtcgatga gaagagtctt ctggctggtc
    21901 gctgccgctc tgttattggc agggtgtgca ggcgaaaaag gcattgtcga gaaagaggga
    21961 tatcagcttg atacccgacg ccaggcgcag gcggcgtatc cgcgcattaa agtgctggtg
    22021 atccactaca ccgcagatga ttttgatagc tcgctggcga cactgaccga taagcaggtc
    22081 agctcgcatt atctggtccc tgcggtacca ccgcgataca acggtaaacc gcgcatctgg
    22141 caactggtgc cggaacaaga actggcctgg catgcgggga ttagcgcctg gcgcggggca
    22201 acgcgcctta acgacacctc tattggcatt gagctggaaa accgtggctg gcaaaaatcg
    22261 gccggagtga aatattttgc cccgtttgaa ccggcacaga ttcaggcgct tattccactg
    22321 gcgaaagata ttattgcccg ttatcacatc aagccggaaa acgtagtggc acatgcggat
    22381 atcgcaccgc agcgcaaaga cgatccgggg ccattatttc cctggcagca actggcgcag
    22441 caggggattg gtgcctggcc ggatgcgcag cgggttaact tttaccttgc cgggcgcgcg
    22501 ccgcacacac cggtagatac cgcgtcattg ctggagcttt tggcgcgcta cggttatgac
    22561 gttaaacctg atatgacacc gcgcgagcag cggcgcgtga ttatggcatt ccagatgcat
    22621 ttccgcccga cgttatataa cggcgaagcg gatgcagaaa ctcaggcgat tgccgaagca
    22681 ttgctggaga aatacgggca ggattagcgc ggcagttttc cgtggtcgcg tagccaggcg
    22741 gcagttttct cgataccttc atccagggtg atgaccggct gataacctaa ctcttcctgc
    22801 gcacgcgtaa tatccagcgt aaagtcaaaa ttcaacttgg agacgccgta gtgggtcagc
    22861 ggcggctctt ttgctgactt gcggcctaaa cgctccatgc tgcgggcgat catatccagc
    22921 atcgggtagg ggacggaacg aatacgacag tcaatattca actcgtcgat cagcttctgc
    22981 acgatgctgc gcagtgtgcg atgctcgccg ttggtgatgt tgtacacacg cccggaaggt
    23041 agcttatcgc aggcttcctg gcttgccagc cacattgcgt gcacggcatt ttcatagtag
    23101 gtcatatcga ccagcgcact gccgccatgc ggtaacagaa tactgccgta gtggtgcatc
    23161 atatgcgcca gacggggaat aaagacttta tcgtgcggtc cgaacagact ttgtgggcgc
    23221 agaatagtaa agcgcgtttg tggattcgcc tgcgaaagca tattgatcac ttcttcgctg
    23281 gctgctttgc tgcgggcaaa ctcgttggcg aagcggtgag ggcgaaaatc ttctttaata
    23341 tcgcgatggt ggtgataatc gaagtacagg gagggggaag agatatgaat aaagttacgc
    23401 acaccccagg cgacagccca ttcacccagg cggcgagtgg cgcgaacgtt agccagatcg
    23461 aaagcctgtt gtgtccccca gggtgaggta aagctggagc agtgccacag cgtatcaatg
    23521 cccgcgagca tcactttagc ttgtgatgaa accagctcgg tcagatccgc cggaacaaac
    23581 tctgcgccca ttttttccag caatttgccc attgcctcgt tgcgaccggt cgctcgcacg
    23641 ctgatgcctt tctggcataa aaactctacc gcgtttcgac ctaagccgct ggtggcgccg
    23701 gtaaccagta ccttcatatc aatccactgt tgttgagaaa ataacgtgcg cattcttccg
    23761 tgatttcccc catgatgcaa tgggaaacat gaaagaataa cgcaggtttt gtcgattaat
    23821 ctgtgctttg ttctgccagt ctggcgattt gttttgccat tccgcgaaaa ataaacagat
    23881 gcgcggggat catcaataac cagtaaaaca gccccggcat accgtgcgga tgccagaaag
    23941 cgcggacatc gatagtacga tagtcgcctt tatcttccag gctaaaacac agtcgtccca
    24001 gccccggcgc tttcatgcca aataacaacg taagttgttt ttccggttca acgacaatca
    24061 ctttccagct atccaccgca tcgccagtct gtaaatattc gcgctccggg cggcctttcg
    24121 ccagcttatg accgatcgcg cggtccatca acgcccgtgt ctgccacaaa atattgccaa
    24181 agaaataacg ctctttaccg ccgatttggt tcactacctg ccataaagca gcaaggctgg
    24241 cggacgtttt aacggtaaac cccgcctgtt tggcaaaata accgtactcc ggtcgccagc
    24301 gggcaaaggc ctgagcgtcg tagccccagt cgctggagtt gaccagtttt tcctcctctt
    24361 tcaacgtgct acgtaccgcg tcatcgaaag cgatcagccg ttgtgggatg agtgcacgta
    24421 gcgcggtatc atccgccagc agatcgtgtt tcagcccctg aatcaacgcc ctggcggtgg
    24481 tgggcggtac ggaagtaatc acattgagaa accacaccga aatccagcgg gtggggaggg
    24541 ggatggggat caaccagcgg cgcttaccgc tcaccgccat aaaatgttca aactgttgct
    24601 gataactgag cacctctggt ccggcggctt cgaagatgcg gtgttcgctg gccggatgat
    24661 ctaacaacgc aaccagatag tgcagcaagt tttccagcgc aatgggcgtg gtgcgtgaac
    24721 gaacccagcg cggaggcgtt aagactggca ggttgtagac catatcgcgc atgacttcga
    24781 acgccgcgga acctgcgcca acgataattc cggcccgcag ttcggtcacg ggtacattcg
    24841 cttcacgcag aatgtccgcc gtagcctgac gagcacgcag atgatccgac tgctcatgtg
    24901 gcggggcctg caacgaacta agaaagatta attgcttaac tggtacttca cgtagcgcat
    24961 cgcggacgtt gagagccacc tggcgctcct gagcgataaa atcgccgcct tcgcccatgc
    25021 tgtgcaccag aaaatagacc gtatcgatat cctgcaacag ggccggtagg ttatccggcc
    25081 agctgagatc gactttatgg caactgacgt ttgccagttg cagctttgca agcctgtcga
    25141 catgacgtgc cgccgccagg atctgatgcc cttgctggct gagtgtgcgc accagatgct
    25201 gaccaatgta gccactggca ccgagaacta aaatgcgttg cggcacgtct ctctccttaa
    25261 cgcgccagga atgcacgcca gtgggcggcg acttccgcca gttgttcgcg cgagacgtca
    25321 agatgcgtca ccaggcggac aatcggcgag gcgttaatca gcacgtttct cgctttcatg
    25381 tattcgccta acgcggcagc attttcttcc ccgacgcgaa caaacagcat attggtgtcc
    25441 tgacgcatca catccgcgcc tgcttcacgc agctgctccg ccatccaggc agcgttgtcg
    25501 tggtcttcct gcaagcgcgc aacgttattt ttcagggcat atatcccggc ggcagccaga
    25561 atgccggact ggcgcatccc gccacctgtc attttccgcc agcgaatggc acgtttaatg
    25621 taatcacgat taccgacgag taatgaaccg actggcgtcc caagaccttt cgacaggcaa
    25681 atggtgaacg aatcacaata ttgcgtgatc tctttcagtt cgcagccgta agccaccacg
    25741 gcattaaaga tgcgcgcacc gtcaacatgc agcgccagat tgcgctcgcg ggtaaattcc
    25801 catgcttctt tcaggtattc ccgcggcaac actttgccgt tgtgggtgtt ttccagactg
    25861 agtaatttgg tgcgggcgaa atggatatcg tcgggtttga ttttcatcgc cactttatcc
    25921 agcggtagcg tgccgtcggc agccgcgtct atgggttgcg gttgaatact gcccagcacc
    25981 gccgcgccac cggcttcaaa cagatagtta tgcgcggcct gaccgacaat atactcttcg
    26041 ccgcgttcgc agtgactgag cagagcgacc aggttggcct gagtgccggt aggcagaaaa
    26101 atggcggctt ctttaccgga aagctctgct gcgtagtcct gcagagcatt aacggtaggg
    26161 tcgtctccgt aaacgtcgtc cccaaccggg gcggccatca tcgcttcgag catggcgcgg
    26221 cttggtcggg taacggtatc actgcgtaaa tcaatcatgg catgtcctta ttatgacggg
    26281 aaatgccacc ctttttacct tagccagttt gttttcgcca gttcgatcac ttcatcaccg
    26341 cgtccgctga tgattgcgcg cagcatatac aggctgaaac ctttggcctg ttcgagtttg
    26401 atctgcggtg gaatggctaa ctcttctttg gcgaccacca catccaccaa caccggaccg
    26461 tcgatggaga aggcgcgttg cagggcttca tcaacttcag acgctttttc tacacggata
    26521 cccgtaatgc cgcacgcttc ggcaatgcgg gcaaagtttg tgtcgtgtag ttcggtgccg
    26581 tcagtcaaat agccaccagc tttcatctcc atcgccacaa agcccagcac gctgttgtta
    26641 aagacgacaa ttttcactgg cagtttcatc tgcactactg agaggaaatc gcccatcaac
    26701 atgctaaaac cgccatcgcc gcacatggcg accacctgac gttctggctc tgtcgcctgc
    26761 gcacccagcg cctgcggcat ggcgttagcc atcgaaccgt ggttaaacga acctaacagg
    26821 cgacgcttgc cgttcatttt tagataacgt gccgcccaca ccgttggcgt accaacgtca
    26881 caggtgaaaa tagcgtcatc ggcggcaaaa tgactaattt gctgcgccag atattgcggg
    26941 tgaatggctt tctcgctcgg tttagctaaa tcgtccagcc ctttgcgggc gtcgcggtaa
    27001 tcttccagcg ctttatccag aaacttgcga tcggcttttt cttccaccaa tggaagcaat
    27061 gcacgcagag tcgacttgat atcgccgacc agtgccatat ccaccttgct gtgagctccg
    27121 atgctggctg ggttgatatc aatctgaatg attttggcat cggtcgggta gaaggcgcgg
    27181 tagggaaatt gcgtgccgag tagcactaac gtatcggcat tcatcatagt gtgaaaacct
    27241 gacgagaagc cgattaaccc ggtcattcca acatcatacg gattatcgta ttcgacatgt
    27301 tctttaccgc gcagggcatg aacaataggt gctttaattt tcccggcaaa ctcaactaac
    27361 tctttatgcg cccccgcgca gccgctgcca cacatcaggg cgatattgct ggaataacgc
    27421 agcagttgcg ccagtttgcg taactcttct tcttccggcg tcacaactgg ctgtggcgca
    27481 tgataccagt gggtagttgc tccttctggc gcaggtttta acgccacgtc acccggtaac
    27541 acaacaacgg aaacgccacg gttaagcact gctttgcgca tagcaattgc cagcacttgt
    27601 gggatctgct ccgggctgga aaccagctcg caatagtgac tacattcgcg gaatagctct
    27661 tgtgggtggg tttcctggaa atagccgctg ccaatttcgc tggagggaat atgagcggca
    27721 atcgccagta ccggaacgtg attgcggtgg caatcgaaca ggccgttgat taagtgcagg
    27781 ttgccggggc cgcacgatcc ggcacagacc gccagttctc cgctaagttg tgcttcagcg
    27841 ccagcggcaa aggccgccac ttcttcgtgg cgggtggaca tccactcgat ggtgcccatg
    27901 cgattaagac tgtcactaag accgttcaga gagtcgcctg tgactcccca gatgcgtttc
    27961 acccctgccg attcgagtgt tttggcgata taagctgcaa ccgtttgttt catggttctc
    28021 catctcctga atgtgataac ggtaacaagt ttagttcatc tgacggaggg ggaagggatg
    28081 ggagagaaag gaggcactaa cggttaaata gcccgatgaa aggaatatca tcgggcataa
    28141 ggcgattatg cgagaaccaa atccccctgc ggatggcagg agcaggccag tacgtaacct
    28201 tcagcgattt cggcgtcggt cagcgtcatt gtgctgctca ccgtatattc accggaaacc
    28261 acttttgtct tacagcagcc gcaaacaccc gcacggcagg cagcgacaac cggaacgtta
    28321 ttgctttcca gcgcctccag tagcgtggtg ccaaccgggg cgtaaaattc tcgtgccggt
    28381 tgcagtttgg tgaatttcag accgctggtc gccgcttctg ctactggggt gaagaatttc
    28441 tctttaaaga aacgcgtcac gccgagcgct ttcacttcct gctctaccca atccatatac
    28501 ggagccgggc cgcaggtcat cacggtacgt gaagctaagt caggtacacc tgccagcagt
    28561 tcgcgagtga gacgaccagc gataaagcct tcggtaacgt tattttctgc caccagcgtt
    28621 accggatagt tacgccactc atcggcgaaa ataacatcct gcggcgtacg cacgttgtag
    28681 atcacccgca catcggcctg tggacggttc ttcgcaagcc agcgacgcat cgacataatc
    28741 ggcgtgacgc cgcagcctgc cgccagcaac aggaatttat cttctgcttt atcgtcgcag
    28801 gtaaattccc ccatcgcgtc cgaaagccag agataatcac cgcgttttac atcgcgcgtc
    28861 agccactggg agccgacacc gtcatcaatc cgccgcacgg tcagggtgat atattcactc
    28921 acgcctggcg tggaggaaat ggtgtaagca cgcagcgttt ccgctgagtt tcgcacgctg
    28981 accagtgcat attgcccggc gcgatatggg tagtaatcgt ggcaaatcag ggaaatcgtc
    29041 cacacatccg gcgtttcttg cgtaatgtga tgaacctgca tccgccacgg gcattgattc
    29101 gttggcatcg tcatcgacaa actccttacg cgctcaacag ttgcttcatg tcttcttcaa
    29161 cagtggtgat agaacgcagg ccgaatttct cgttcagcac cgccagcagg tctggtgtca
    29221 ggaaaccagg tgcagtcggg ccggtgacga tatttttcac gcccagagaa agcagcgtca
    29281 gcagaatgac gatcgctttc tgttcaaacc aggagagcac cagcgacagc ggcagatcgt
    29341 tgacaccgca gcccagtttc tctgccagag tgacagccag aataatcgct gagtaagcat
    29401 cgttacattg acctgcatct accagacgcg gcagaccttc gatatcgcca aactcaagtt
    29461 tgttaaagcg atatttacca caggcgaggg tcaggatcag gcagtcatcc ggcacgctgg
    29521 tggcgaaatc ggtgaagtag tggcgctcgc cgcgtgcgcc gtcacagcca ccaagcagga
    29581 agatatgacg cagtttttca cggctcacca gatcaatcag cgtatcagca gcgccaagca
    29641 gcgtctggcg accaaaaccc acggtgataa ggtgcggaat ttcgctgtac gggaagcctg
    29701 ccatctgttg cgcctgggtg ataaccgcag agaaatcatc accgtccaga tgacgcacgc
    29761 caggccagcc aacaatgctg cgggtccaga tacgatcgtc ataagcgcct acggttgggt
    29821 cgatgatgca gttcgaggtc atcacgatgg ggccagggaa acgagcgaac tccacttgct
    29881 gattctgcca gccgctgccg tagttaccga ccagatgctt gaatttacgc agctccgggt
    29941 agccatgcgc aggcagcatt tcgccgtggg tgtagacatt aacgcccgtg ccttcggtct
    30001 gttccagcag gttgtagaga tctttgagat cgtgaccgga aatcagaatg catttacccg
    30061 ccgtcgcttt gacgttgacc tgggttggcg tcgggtgacc gtatttaccg gtttcgcctg
    30121 catccagaat gctcatcact ttgaagttca tctggccgat ttccattgaa cactcaagaa
    30181 gcgcgttcat atcggcaggc caggtcccca gccacgccat gattttatgg tactgggcat
    30241 aaatatcgtt gtcgtattga ccgagaacat gcgcgtgttc catataggcc gccgcacctt
    30301 tcaggccata caggcacagc agacgcaggc cgagaatgtt ttcgccaatc gccgctttat
    30361 ctttgttagg ggtaaattct gctgcctgac gttgcagctc gccgagatcg tcgctcacca
    30421 gttgcaggtc agccatcggg ttatcgacgc gcgcgttggc atctacagcc aggcattgcg
    30481 ctttcagcgc ctcgcgcagg gcaatcgctt cacgagcgta gccgacaata cgcggagaat
    30541 cgaagttaac gttggtcagg gttgagaaaa aggcacgtgg cgcgaagctg tcaacatcgt
    30601 ggttgatgat gccgtattca cgcgctttta ccgcccaggc agaaagccct tgcagcgccg
    30661 cgatgagtaa atcctgaagg tcagaagttt ccgccgtttt accacacatc ccctgcgcgt
    30721 atgagcagcc gtttcctgcc ggagtacgga tagtttgttc acattgcaca caaaacatga
    30781 tcacaccttt taaagttata tttaatatac atgtttaagg ttaagacgct taacgcgggg
    30841 ataaaaggga tttttcatgc aactttaagg gagattgatt tagcgcaatt ttggcggcag
    30901 ggatctaccg ccagagaggt attacgcaga gaaaaaggcg atgaggatcg gcactaacag
    30961 gctaagaata aaaccgtgaa caattgccgc cgggaccata tccagcccgc cagtacgttg
    31021 aagaacgggc agggtgaaat ccattgatgt ggcaccgcat aagcccagtg cagtagagcg
    31081 gctgcggcga atcagcccag ggatcaacat aatagcaatc agttcacggg ccagatcatt
    31141 aaaaaacgcc gcgctcccga ttaccgggcc aaaagattcg gtcaataaaa taccggaaag
    31201 agaataccag ccgaaaccgg aggccattgc cagcgcggta ttgatgggga gatcaagaat
    31261 aaaggcgtta attaaaccac caattaatga actggcaacc accaccacgg cgacaatcat
    31321 tccccggcga ttaaggacaa tctgctttaa ggtcatgcca ttattgcgca actgaatacc
    31381 gacgaggaaa agtagcagaa ttaacgtgta ttcgctggct tcggtcgcgt gttgtaagaa
    31441 agccagtcca ctcagaccaa tggcaaaacc aatcactact acgccgcaca gttttagcga
    31501 ctccagcgcc atcgcaatac gcgacgggag tttttcttgc tgatggtgat tgcgccatgg
    31561 caggcctcgc tccagccaca tcagggcggc aatattacat agtaaaataa cggtaatact
    31621 gacagcagaa taatgcagaa tcgccaacag gttactggcg aggttatcga gaaacgccag
    31681 actgataccc ataaaaaaga gaataaggta aaccatccag cttaatagct gattaataac
    31741 ttttaatgca gctttttggc gaagcggaat gaggtaaccc acaatcaggg gaaccagaat
    31801 gattaacagc ccagaaaaca tgaaaaccca gtccttgcaa agatgaagtc gaaatgcgcg
    31861 atgacacact actgaaagcg gaaggacgag taaagttgca attaaaagga aatgttatgc
    31921 atcagagggg gatttttaat cctatctgag tgtttagcat tgttgcgctc ctttcatgtg
    31981 caatgccata taaagccact ggcgcaggga gcgcgcaggg ggcggccagt cgccgccgcc
    32041 ccctgcaccc ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc
    32101 ccttcggcta ccgggtcggg cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat
    32161 ccatgtgggc tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaacaccc
    32221 gtgcggtttg taacgtcgaa gataaaacaa ccagggcaga taaaatgcca atgctaatta
    32281 attacgtttt tccagcaggg tccggtaaat cagaccaccg ataatgccgc cgacaattgg
    32341 caccacccag aagaaccaca gttgttctaa tgcccagccg ccctggaaga tagcaaccgc
    32401 ggtgctgcgc gccgggttaa cagaagtgtt agtcaccgga atactgatta agtgaatcag
    32461 ggttaaggcc agaccaatag cgatcggcgc aaaacctgcc ggagcgaatt tgtcggttgc
    32521 gccgtggatc accaacagga aacctgcact caataccagt tcaactacca gcgcggaaag
    32581 catggaataa ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc
    32641 cgcgtcaaaa cccgttttac cactggcaat taaatacagc agcgctgccg caacaatacc
    32701 gccgacaacc taggcaatta cgtagccaac gacttctttt gccggaaaac gtccgccagc
    32761 ccataaacca atagtgaccg ccgggttaaa atgaccacca gaaatatgac caacagcaaa
    32821 ggccatcgtc agaacggtca gaccgaacgc caacgccacg ccggcaaaac caatgcctaa
    32881 ttccgggaag cctgcggcca gtacagcact accacagcca ccaaaaacaa gccagaaagt
    32941 accaaaacat tcagctgcta attttctgaa catatccacc tcaattaaaa attgaccctg
    33001 tgaaaaatat ggtcgtttta tagggtcgtc gtaaaaaagt gacgacggaa ataatgcgcg
    33061 gctattttaa aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt
    33121 agggcaacag cgggttgccc catatagtca tttgtctgat tgacagtgta gtgc
//
LOCUS       DAJOSY010000045        32909 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJOSY010000045 DAJOSY010000000
VERSION     DAJOSY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.045"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..163
                     /locus_tag="OHN27_002189"
     CDS             <1..163
                     /locus_tag="OHN27_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286625.1"
                     /translation="TITLVILFCRRPHCRKISVRQYYQKRVIFYPTTLQRTACKKCLF
                     LMRDIIISS"
     gene            124..243
                     /locus_tag="OHN27_002190"
     CDS             124..243
                     /locus_tag="OHN27_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCQ2286626.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            278..621
                     /locus_tag="OHN27_002191"
                     /pseudo
     CDS             278..621
                     /locus_tag="OHN27_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(993..1259)
                     /gene="minE"
                     /locus_tag="OHN27_002192"
     CDS             complement(993..1259)
                     /gene="minE"
                     /locus_tag="OHN27_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCQ2286627.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1263..2075)
                     /gene="minD"
                     /locus_tag="OHN27_002193"
     CDS             complement(1263..2075)
                     /gene="minD"
                     /locus_tag="OHN27_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCQ2286628.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2099..2794)
                     /gene="minC"
                     /locus_tag="OHN27_002194"
     CDS             complement(2099..2794)
                     /gene="minC"
                     /locus_tag="OHN27_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCQ2286629.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPTPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3314..3682
                     /locus_tag="OHN27_002195"
     CDS             3314..3682
                     /locus_tag="OHN27_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCQ2286630.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPALTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3785..4186)
                     /locus_tag="OHN27_002196"
     CDS             complement(3785..4186)
                     /locus_tag="OHN27_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCQ2286631.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4428..4721
                     /locus_tag="OHN27_002197"
     CDS             4428..4721
                     /locus_tag="OHN27_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCQ2286632.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4793..5452
                     /locus_tag="OHN27_002198"
     CDS             4793..5452
                     /locus_tag="OHN27_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCQ2286633.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     STADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5544..5990
                     /locus_tag="OHN27_002199"
     CDS             5544..5990
                     /locus_tag="OHN27_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCQ2286634.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6196..7107)
                     /gene="hlyE"
                     /locus_tag="OHN27_002200"
     CDS             complement(6196..7107)
                     /gene="hlyE"
                     /locus_tag="OHN27_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCQ2286635.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYIFLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGIVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            7480..7899
                     /locus_tag="OHN27_002201"
     CDS             7480..7899
                     /locus_tag="OHN27_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCQ2286636.1"
                     /translation="MLFIKPADLREILTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            7899..9167
                     /gene="umuC"
                     /locus_tag="OHN27_002202"
     CDS             7899..9167
                     /gene="umuC"
                     /locus_tag="OHN27_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCQ2286637.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(9213..9743)
                     /gene="dsbB"
                     /locus_tag="OHN27_002203"
     CDS             complement(9213..9743)
                     /gene="dsbB"
                     /locus_tag="OHN27_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCQ2286638.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(9889..11430)
                     /gene="nhaB"
                     /locus_tag="OHN27_002204"
     CDS             complement(9889..11430)
                     /gene="nhaB"
                     /locus_tag="OHN27_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCQ2286639.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAKHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            11651..12370
                     /gene="fadR"
                     /locus_tag="OHN27_002205"
     CDS             11651..12370
                     /gene="fadR"
                     /locus_tag="OHN27_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCQ2286640.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(12422..13954)
                     /locus_tag="OHN27_002206"
     CDS             complement(12422..13954)
                     /locus_tag="OHN27_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCQ2286641.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            14284..15582
                     /gene="dadA"
                     /locus_tag="OHN27_002207"
     CDS             14284..15582
                     /gene="dadA"
                     /locus_tag="OHN27_002207"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCQ2286642.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            15592..16662
                     /gene="dadX"
                     /locus_tag="OHN27_002208"
     CDS             15592..16662
                     /gene="dadX"
                     /locus_tag="OHN27_002208"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCQ2286643.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(17049..18785)
                     /locus_tag="OHN27_002209"
     CDS             complement(17049..18785)
                     /locus_tag="OHN27_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCQ2286644.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQHHESNISWMFIVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFSLTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(18880..19794)
                     /gene="ldcA"
                     /locus_tag="OHN27_002210"
     CDS             complement(18880..19794)
                     /gene="ldcA"
                     /locus_tag="OHN27_002210"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCQ2286645.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            19894..20505
                     /gene="emtA"
                     /locus_tag="OHN27_002211"
     CDS             19894..20505
                     /gene="emtA"
                     /locus_tag="OHN27_002211"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCQ2286646.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(20507..21241)
                     /locus_tag="OHN27_002212"
     CDS             complement(20507..21241)
                     /locus_tag="OHN27_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCQ2286647.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMRQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            21442..21696
                     /locus_tag="OHN27_002213"
     CDS             21442..21696
                     /locus_tag="OHN27_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCQ2286648.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            21874..22314
                     /locus_tag="OHN27_002214"
     CDS             21874..22314
                     /locus_tag="OHN27_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286649.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(22393..24090)
                     /gene="treA"
                     /locus_tag="OHN27_002215"
     CDS             complement(22393..24090)
                     /gene="treA"
                     /locus_tag="OHN27_002215"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCQ2286650.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTHGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(24410..25828)
                     /gene="dhaM"
                     /locus_tag="OHN27_002216"
     CDS             complement(24410..25828)
                     /gene="dhaM"
                     /locus_tag="OHN27_002216"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCQ2286651.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(25839..26471)
                     /gene="dhaL"
                     /locus_tag="OHN27_002217"
     CDS             complement(25839..26471)
                     /gene="dhaL"
                     /locus_tag="OHN27_002217"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCQ2286652.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(26482..27552)
                     /gene="dhaK"
                     /locus_tag="OHN27_002218"
     CDS             complement(26482..27552)
                     /gene="dhaK"
                     /locus_tag="OHN27_002218"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCQ2286653.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVTG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVNVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            27780..29699
                     /gene="dhaR"
                     /locus_tag="OHN27_002219"
     CDS             27780..29699
                     /gene="dhaR"
                     /locus_tag="OHN27_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCQ2286654.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSVLLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(29799..32684)
                     /locus_tag="OHN27_002220"
     CDS             complement(29799..32684)
                     /locus_tag="OHN27_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin family autotransporter"
                     /protein_id="HCQ2286655.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDAIINFTGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNDGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPTPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMSYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVR
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGYSQLNMYVKTGAIREFSGDTEYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYHFSF"
BASE COUNT         8194 a         7962 c         8332 g         8421 t
ORIGIN      
        1 aacgattaca ttagtcattt tattttgccg acggcctcat tgtcgaaaga taagcgtacg
       61 acagtattat cagaaaagag tgatttttta tccaactaca cttcagcgca ctgcgtgtaa
      121 aaaatgcctc tttcttatgc gggatatcat catttcatca tgatgtcttt ggtgagcggt
      181 gaacacaata cacctgcgct gtctcttcag gatgaatccc ctggtctggt gcctgcgggc
      241 tgatgttgca gcagagctta ggtcacttag acgctactat catttatcca atggcatgga
      301 atcgaaatca gtcgataccc gcagtatata tcgtgaactg ggtgcaacgc tgagttacaa
      361 catgcgcctg gggaacggta tggaaatgaa ccctggctga aggcggctgt gcgcaaagaa
      421 tttgtcgatg ataaccgggt gaaggtgaat aatgacggta atttcgtcaa tgatttgtcg
      481 ggcagacgtg gaatatacca ggcagctatt aaagcctcat tcagcagtac gtttagcgga
      541 catctcgggg tggggtatag ccatggtgcc ggtgtggaat ccccgtggaa cgcggtggct
      601 ggtgtgaact ggtcgttctg accatcaacg attaaactgc gcttcggcgc agttttcgtt
      661 tacaggatgt tgaaagggaa aattctgggg caaaaaaagc ccgccagtta cggcgggaaa
      721 cctcatccta tgggagaaca atgaataatg aaattgcggg gttatcatct cccagtatat
      781 ccatactaac aataaggtta tttactcaac caggcataaa cattttgttt tgtgcgtggg
      841 aacagcctta aggtgtaaag ggggaggtgg aaatagcaat gaggagtatc agcaagaata
      901 ctcgccgctt taccacaacg tggatgagag ggatgaaaaa ctcaaggcag agataactct
      961 gccttgaaga taaatgcgct tttacagcgg gcttatttca gctcttctgc ttccggtaag
     1021 gtcacgttca gctcaagaat agaaatatcg ccatcttttt gctcaagctg tacggttacc
     1081 atctcaggat caatttgtac gtatttacaa atgacctcaa gaatatcttt acgcaactgc
     1141 ggcagataat gcggttctgc atcgctgcga cggcgttcag caacaataat ctgcagccgt
     1201 tcttttgcaa tgttggctgt gtttttcttc cgcgagagaa agaaatcgag taatgccata
     1261 acttatcctc cgaacaagcg tttgaggaag cctttcttct cttcttcaat gaagcggaaa
     1321 ggacgttctt ctcccaacag acgttctacg gtatctgcgt aggctttacc cgcatcggcg
     1381 ttaatgtcga gaatgaccgg ttcaccctgg ttagaggcgc gcaatactga ttgatcctct
     1441 gggatcacgc cgacgagttt gatgcgcagg atctccagca catcttccat gctcagcatg
     1501 tcacctctgc ttacgcggcc tgggttatag cgcgttaaca gcaggtgctc tttaataggc
     1561 tcttcgccat tttctgcgcg gcgtgatttc gacgccagaa tgcctaaaat acggtcagag
     1621 tcgcgtactg aggagacttc cgggttggtg gtaataatgg cttcgtctgc aaaatagagt
     1681 gccattaacg caccggtttc aatccctgcc ggggagtcac aaacgataaa ttcaaaatcc
     1741 atcgctttca gatcatcaag aactttggcg accccttcac gggtgagggc atctttatcg
     1801 cgtgtttgcg atgccggcag aatatagaga ttttcagtac gcttatcttt aattaacgcc
     1861 tgatttagcg ttgcatcgcc ctgaatgacg ttgacgaaat cgtaaacgac ccggcgttca
     1921 caacccataa tcaggtcgag attacgcagg ccgatatcaa aatctatcac gacagttttc
     1981 tttcccttct gggccaaacc agtggcgatg gccgcgctgg aggttgtctt accaacaccc
     2041 cctttgcccg aagtaacaac aataatgcgt gccatagaaa ttccttgtta aaaagggatc
     2101 aatttaacgg ttgaacggtc aaagcgtttt cgactaactg cagtcgcgcc gctttgccat
     2161 aaaattctgc tgggatttga tcactcagcc agtattcacc tgcgatggac accagttccg
     2221 ccatcaggtt cgtacaaaat atttgcgttt cccggtcacc acttgcccct gccagcgcac
     2281 gaccgcgcat catgccatag acatgaatgt tcccatcggc aatcaattcg gccccagcgc
     2341 taacgtggct tgtaacaatc agatcacatt gtggagcata aatacgctga ccggaacgca
     2401 ccggggtatc tattaaacgc gtttttgtga ccggcgttgt attttgcgct ggagcctgcg
     2461 gtgtgggagc tggacgtggc gctttttcct ttccttccgt caggataggc agccccatct
     2521 tttcaatttc ggctttaagt tgcgcatctt tgcagccact tacgccaata acccgcaaac
     2581 cggttgccga aaccgcctta tgcatcgctg accagtttac cgggtcttcc agtgcactga
     2641 cgttgagtac aacgggggca tgttttaaaa atgcgggggc ctgagcgatt ttgtcttcca
     2701 gcgcctgatg gataacctta ggttctgcct catgcagatg aaccacagat aaagtgaagc
     2761 tactgccttt aagctcgatt ggcgtgtttg acatcctggc cttactcaat tagctattaa
     2821 tcatcgccag cgcgcgatga tgttccgaag actataaggc atgttatagt ctggattata
     2881 ttgaggcaag tcaccctccc atttattcag agtaaaagtc tattctgtga taaatggcgc
     2941 tgattcatag cttaaaaaat acccttgtca atcaacccat tgccgtcgta cttttgattg
     3001 ttcttattta cgcttccttt ttccgcaccc taatcgtgac agcatctcca gacctgttta
     3061 aatatccagt attgaggcaa gtgtattttt tcaccacgcc aggctttctt aactttccat
     3121 tttttgcggc atcattaaag aacagatacc cgttatctta atatccgcca cagaattgct
     3181 ctatcataga aaccgcagaa gacgtctgat acttctgtca ggtcatcgta actcgtccag
     3241 gtcagcggtt ttgattacgg attgatgatg aaatgagcat aagtgttgta cggttggatt
     3301 atcaggatgt gctatgaata tgatgcgtat tttttatatc ggattgtcag gtgtggggat
     3361 gatgttctca tcaatggcgt ctggaaatga cgctggcgga cttcaatctc cggcgtgcgg
     3421 cgttgtttgc gacccgtata tttgtgtgaa ctcagatggc atttccccag cgttaacaag
     3481 gaaatatctc ggcgaaaaag ccgctgaaaa cttacaatca ttacaaggct acgatcccag
     3541 cgaatttaca ttcgctaacg gtgtattttg cgatgttaaa gaaaaattat gtcgtgatga
     3601 tcgttatttt ggtgtggatg gtaagcggag tggaaaaatc aatcaaacca ccacaaagat
     3661 gttatttatg tgtcgtgagt gatgtcttgt ttacattatg ttctattcgt cattaacctc
     3721 ttgagcgaca gagaggagtg gggcgtcaac gtttatgacg gggagagtcc ccgtcggttg
     3781 acatttactt aatttgaata tcgacgttat attttttagt tttgttttta cgggcgtcgt
     3841 ttcgggtttg cagcactcgc agctcatatt tgccagaagc agggagtgag tattgtccgt
     3901 gactatcgag ttctggggaa tatctggata aatcaacaga atcatcgatt cctggcccga
     3961 agaggtaagt atcagcgcct tcattagaga tactcacatg cactttctga ccttttttgg
     4021 catagaaggt atatgtatcg taatcgtatc cctttatttc gccggagtac tgagcgctgc
     4081 tatggccttt tctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
     4141 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
     4201 tcagaatgag tacgatgaac tgttataata taacaatccc ttacggggga attccagtgg
     4261 ctttcctgcg ttcggtaaaa tcccgtcgcg gcatttttgc cggggggatt acttataaag
     4321 ggggcatgga ctcaacaaac cgtagaaatc tacagctggc tggcttgtgg cgcgggtttc
     4381 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgtga
     4441 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
     4501 cgcgtgttcc tgaggaattg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
     4561 cgctggatgg gcggaagaaa ctggtcaatg ccgatattga aaaggttaaa caggcattaa
     4621 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
     4681 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
     4741 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
     4801 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
     4861 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
     4921 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
     4981 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
     5041 ggctacggaa gagcatgtcc gcaaagccat tgccggttat ggcgtggcgc tcgatctgac
     5101 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     5161 gtttgataac tcttgtccgc tttccgggtt tattcccgcg gcggaattca ccggcgaccc
     5221 gcaaaataca acgctgggcc tgagcgtaaa cggcgagcaa cgccagcaag gttcgactgc
     5281 ggacatgatc cataaaattg ttccgctgat cgcttatatg agcaagtttt ttaccctcaa
     5341 agccggtgac gttgtgctga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     5401 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     5461 gccgcctgaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     5521 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     5581 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcctgc
     5641 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     5701 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     5761 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctaccaatg acctgcgctt
     5821 atcgtttgct ggcggaaggt aaagatttac ctgcgtggca tccgctgctt actggttcga
     5881 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     5941 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     6001 agatgttgat ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     6061 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     6121 aatctttaaa aaacgtctgt atttttaaat gtgataaact ttataccttg agtacatgga
     6181 gagaataatc gctgatcaga cttcaggtac ctcaaagagt gtctttttac cgtgtctttt
     6241 ctgatactca ttacaggtgt taatcatttt tttggccgct tcttttagta aagaaagcat
     6301 taaatcatca taatcaacgt agaatctggt tgtttcagtt tccgttttta tctctccgat
     6361 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     6421 aaccgtgttt gacagggtgg taaagaaatt ctgcacagat tttaacttgt tcttcaattc
     6481 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     6541 tggaccggcg acgataccgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     6601 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     6661 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     6721 ttcattcagt ttcgtgatgc cgtcatccag taccttaatg agaatgtctt tctgggcgga
     6781 tgctttcttc tcattgtact catcaaatag aaaaatatac gctgcgagca attgcgtcgc
     6841 aacaccacac cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat
     6901 aagtaaggtt ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa
     6961 gcgacttaac tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata
     7021 tttattataa agatctaatg ctccatctgc agtttcgatt gcgtttttaa ccacttctac
     7081 cgttttatct gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa
     7141 tgaaactcct gttttacaac tattaataaa ttttacttaa tctaattcat agttagccgg
     7201 gtgggatgcg tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg
     7261 tcattatggc gaatgcttct attctatttt ttagccgggt gatatttttc atttctgctg
     7321 gatgagcgtc gtcgccagaa ggccgcgtga gcacaggata agagaacgaa aaatcagcag
     7381 tctatgcagc gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac
     7441 tactgtatat aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg
     7501 gatctccgcg aaattttgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct
     7561 tcaccggccg cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat
     7621 cccagcgcga cttacttcgt caaagcaagt ggtgattcta tgattgacgg tggaattagt
     7681 gacggtgatt tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc
     7741 gctgctgttg acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt
     7801 attcccatga acagcgcgta ctcgcccatt accatcagta gtgaagatac gctggatgtc
     7861 tttggtgtgg tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa
     7921 acgcgtttta tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg
     7981 ttgttttatc gaataatgac ggttgcgtta ttgcccgaaa cgccgaggca aaggcgctgg
     8041 gtgttaaaat gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggcgtgg
     8101 tttgctttag cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc
     8161 tggaagagct atcgccccgc gtcgagattt acagtattga tgaggcattc tgcgatctga
     8221 caggtgtgcg taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc
     8281 tacaacgtac ccatcttact gttggcgtgg ggatcgccca gaccaaaacg ctggctaagc
     8341 ttgccaatca tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa
     8401 atctggaacg ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg
     8461 gacggcggat cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg
     8521 atacagatat ccggtttatc cgtaaacatt ttaatgtcgt gctcgaaaga acggtgcgtg
     8581 aactgcgcgg cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta
     8641 tctgttcccg ctcgtttggt gaacgcatca cggattatcc gtcgatgcgg caggccattt
     8701 gtagttacgc tgcccgggcg gcggaaaaac ttcgcagcga gcatcaatat tgtcggttta
     8761 tctccacgtt tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg
     8821 cgtcggtaaa actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc
     8881 gatctctgga tgccatctgg caagcgggcc atcgttacca aaaagcgggc gtgatgctgg
     8941 gggatttctt cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc
     9001 ccgggagtga gcaattgatg acggtaatgg atacactgaa tgctaaagag ggcagaggaa
     9061 cactctattt tgccgggcag gggatccagc aacaatggca gatgaagcga gccatgcttt
     9121 caccacgtta tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg
     9181 aaaaaagcgc tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc
     9241 gctttaaacg gctgggaaat caccaccagc actgcgacaa tcaggtaagc gataaaaata
     9301 ccgagcagcc actgcggcat ttccagacct aaaaaatccc actgacgctc ggcgcaatcg
     9361 ccagaggcga caaacacttg cggcacccac ttatccagcg gcagccattc cgggaaacga
     9421 accataaaat cacaggtggc aaacggcgaa ggatagagct gaagcatggt gtgctcgtaa
     9481 gttaactgca caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc
     9541 ggagttttcg gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag
     9601 cgttcataaa tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc
     9661 gtcagttcca gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa
     9721 cattggttca aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt
     9781 aaaccaattc attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt
     9841 atcgggcgaa aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc
     9901 tatccagccc atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag
     9961 caagccgacg agtgtcagga cgagggtgta aggcagggcc atccacacca tgcggccata
    10021 agagaggcga atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc
    10081 gttcggcgta gcgacggagg gcagattggt accggtatta atggcgaccg ccagcagctc
    10141 gtattgcttc aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat
    10201 cgtccccacg aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag
    10261 cgacagctga gcatgttccg atgcctgcaa cacaaactga ataattggcg aaaacagttg
    10321 ttggtcgata atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag
    10381 agattcggtg aaggctttac cgatagcatg ctcatcggtg acaccggtca atgatgttgc
    10441 cagaataatg actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt
    10501 caccagccag acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg
    10561 ctggtggcgg ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt
    10621 ttcaccgtag ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat
    10681 cagaaccgga acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc
    10741 cgctttagcg atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc
    10801 ggtgccgaca ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac
    10861 cactttgtaa tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga
    10921 agaggctacg cggtgataaa taccataaaa accgactgca acgctgatca ccaccgccac
    10981 gacggttaag gcatcgagga acgcggagag gaacgcagcc gccacgcaaa aagagagcga
    11041 cagcagcatt ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg
    11101 tttcataaaa tagatacccg ccaccataaa catcagtaac agcaagactt caagatttgc
    11161 cgccacctct tcacggacgt gtttcgcgct ggtcatgccg atgaataccg cttcgatagc
    11221 caacagacca ccggggagca gcgggtagca tttcagggcc atcgccagag tgaaaataaa
    11281 ttccgctacc agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac
    11341 gattaagaaa atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg
    11401 ccatagcgcg cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa
    11461 tgatgttttt atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga
    11521 tagtcaaggg gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct
    11581 gctatcagcg tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg
    11641 aaatctcact atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat
    11701 tgaaagtatc tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact
    11761 ttcagaatta attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg
    11821 agatggctgg ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac
    11881 ttccggttta aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct
    11941 tattgataat ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt
    12001 tcgtcagcat cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca
    12061 cgccgatgcc tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg
    12121 caacccgatt tacggtctga ttcttaacgg gatgaaaggg ctgtatacgc gtattggtcg
    12181 tcactatttc gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc
    12241 ggcgttgtgc agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca
    12301 tgagagtggc gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca
    12361 ggggcgataa tcccttccgt ttaaagagca aacccctcaa acgaggggtt ttttgttgtt
    12421 tttacagatt tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt
    12481 tttgctgttc gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt
    12541 tgcgcccccg atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt
    12601 cgccgcgcaa atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt
    12661 gcgacgataa ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct
    12721 ccagataatt atgccgatcg tcatccagca cggtgaagaa gcggaaatca cgcatcactt
    12781 tcggtgacag gaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca
    12841 gcgtttccag ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg
    12901 gcgactgaca aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga
    12961 tgccgctgta ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca
    13021 aaaactccag cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg
    13081 tgtagtgcca gaaggtcgcc cagccttcgt tcatcacctg agtctgtttt tgcggataaa
    13141 aatactggct caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg
    13201 gcgcattttt ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg
    13261 cttcagcaac cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct
    13321 gactttgcag atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt
    13381 tttgcgggcg tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa
    13441 gccgttctac ttcatcaacg ccataacgct cttcgcactc ggtaatatat ttacgggcaa
    13501 aaatcagata atcgacaatc gaactggcgt cggtccagct acggaataag taattgtttt
    13561 tgaagaaaga gttatgccca tagcaggcat gagccatcac cagcgcttgc atggtaatgg
    13621 tgttctcttc catcaggtaa gcgatacacg gattagagtt aatgacgatt tcataggcca
    13681 gtccttgctg accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc
    13741 agtgcggata gttaattggc atgccgacgc tggagtaggc atccatcatc tgttctgagg
    13801 ttatcacttc aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta
    13861 tctctgccag ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg
    13921 tggtgtcctt attcatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct
    13981 ctctgtgtgg agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag
    14041 aatttttttc tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat
    14101 tttatgctgg ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga
    14161 gtcagggaga tgtgagccag ctcaccataa aaaagccgta tgttgaataa tattttcaac
    14221 tgagttatca agatgtgatt agattattat tcttttactg tatctaccgt tatcggagtg
    14281 gctatgcgag ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta
    14341 aatcaggcag gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc
    14401 agtgccgcga atgccgggca aatctccccc ggatatgctg caccgtgggc ggcaccaggt
    14461 gtgcctttaa aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc
    14521 gacggtacgc agttccagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc
    14581 cactacatgg aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg
    14641 aaagcattgc gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa
    14701 ctgttccgta ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat
    14761 gccggcgtac cgtatcagct gctggaatcc agccgcctgg cggaagtgga gcccgcgctg
    14821 gcagaagtgg cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac
    14881 tgtcagctat ttacccagaa tctggcgcgg atggcggagc aggcgggggt taaattccgc
    14941 tttaatacgc ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagtgt
    15001 ggcgatgaag tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg
    15061 atgctcaaag gcattgttga tattccggtg tatccgctga aaggctactc gctgaccatt
    15121 ccaattgcgc aggaagatgg tgcgccggta tccaccattc ttgatgaaac ctacaaaatc
    15181 gccattaccc gtttcgataa ccgcattcgt gttggcggaa tggcggagat tgttggtttt
    15241 aataccgagc tgttgcaacc gcgtcgtgaa acgctggaga tggtggttcg cgatctctat
    15301 ccacgcggcg gtcatgtcga gcaggcgact ttctggactg gtctgcgccc gatgacgcca
    15361 gacggcacgc cggttgtcgg gcgtacacgc tttaaaaatc tgtggctgaa taccggtcac
    15421 ggcacgctcg gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct
    15481 ggtcgcacgc cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt
    15541 acgccatcac gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt
    15601 ccgatacagg ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag
    15661 gccgcgacgc acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt
    15721 gagcgtatct ggagcgcgct cggggccacc gatggctttg cattgcttaa cctggaagag
    15781 gcaataacgt tacgtgagcg cggctggaaa ggaccgatcc tgatgctgga aggatttttc
    15841 catgctcagg atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac
    15901 tggcagctca aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa
    15961 gtgaacagtg ggatgaatcg gttgggcttc cagcccgatc gcgtgcttac cgtctggcag
    16021 cagttgcggg caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgccgaagcg
    16081 gaacatcctg atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg
    16141 gagtgtcggc gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt
    16201 gactgggttc ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat
    16261 atcgccaata ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag
    16321 acgctaaaag cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag
    16381 cgaatcggca ttgtcgccgc agggtacgcc gacggttatc cgcgccacgc gcctaccggt
    16441 acccctgttt tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg
    16501 ctagcggtcg atttaacgcc ttgcccgcag gcgggtattg gtacgccggt tgagctgtgg
    16561 ggcaaggaga tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg
    16621 atgtgcgcgc tggcgctacg cgtcccggtt gtgacggtgt aacttgttgt aagccggatc
    16681 ggaggcaacg tcttctgggt gcaaaaaaat catccatccg gctggtcagc aactgtagtt
    16741 gttaatggtg acagagccat tgcccatgat agtgtccatt aaaaggatgg acactatttc
    16801 cccggaacct gaactcaccg cacaggcgtt ctacataaaa cgcttacgct tcattgttga
    16861 ctcgaactcg acttcagata aatcacgctt tacccttgat ggagcctgta catagatttg
    16921 tgtaattgcc tgattttgat atgttcaatt caacatcaaa tgaaggttaa attatggacg
    16981 acaaacaatt gcaggctcag gctgcgttca gcaaagcatc gcaaccggcg atagatgctt
    17041 cattaaattt aagattcagc ttcctcttca gccacccgta cgccaatctt caacacttca
    17101 ttatcttctt tctcggccac cgtccagatc atcccggcaa actctacctg gtcaccgaca
    17161 accggtgccg cgcctaacaa ctgctggaca atttcaccca gcgtttgctg cttatcacga
    17221 tattctcgcc cgtcttccag gccatatatc agcgcaacat cagcatattt ggcgctggct
    17281 tcgagaatga agtcaccaaa gaagcgttga tccagcgcga ccggcggcga ctggctgaac
    17341 agtttaccga gcgccggaag atcgcgttcc cgaccaatta cacacaacac atcgccttcg
    17401 cgcagtcggg tgctgccggt aggatgaagc aactggttat cacgaaacag tgccgcaata
    17461 cgcgtctctt ttggcatatg caaatcgcgc agtgccgcgc ccacgcacca tttatcggca
    17521 ctcaattgat aaacaaactg ctcccacgga ttttccggat gaatatccag gccaacgcgt
    17581 gacaccggac gtcccactgg cggaaccacc actttggctt ttttagccgc ccacgagagt
    17641 gatgttccct gcaagagcag tgaaaccaga accacaaaga aggcgacatt aaagaacaga
    17701 cgtgcattct ccagccccgc catcatcggg aacacggcca ggatgattgg caccgcgccg
    17761 cgtaatccta cccagctgat aaacacgcgc tcacgcagat tgaaaccgcg gaaggggagc
    17821 aatccagcaa ataccgaaag gggacgggcg aagaatatca tccatgcgga caaaatgagc
    17881 gccggaatgg caataggcag cagatcgctt gggttaacca acagccccag caccaggaac
    17941 atggcgattt gcgccagcca ggcgaggccg tcgaaatttt gcaggatgcc gtagcggttg
    18001 cgaatagggc gattacccag cagaaaaccg cacagataca ccgccagaat accgctacct
    18061 tccagcgcag tagttaatga gaaaatcaga ataccgccac ttaatgccag caatggatat
    18121 aatccggcgg gcagggcgat gcggttgatc atttgcagta gtaaataacc gccgccaaga
    18181 cctattacaa tgcccagacc aaattgttgc agaatatcga caatgaacat ccagctaata
    18241 ttgctctcat gatgctggat catcgcaatc agggtaatcg tcagaaagac cgccattgga
    18301 tcattactgc cggattcaat ttccagcgtc gagccaacac gttcgttaag ccccttacca
    18361 cccagcagag aaaagaccgc tgcagcatcg gtagagccga cgatagcgcc gattaataag
    18421 ccttcaatca aatcaagatt aaacagccac gccgccatca tgccggttaa accagaggtg
    18481 ataagcacgc ccagcgtcgc cagcgacagt gccggtccta acgccacacg aaaggaactg
    18541 gcctgagtgc gcatcccgcc gtcgagcaaa ataatcgcca gtgccaggtt acttaccatg
    18601 taggcgaagg ggtaattatc aaacgggata ccgccgacgc catcgactcc tgccagcatg
    18661 ccgatcgcca gaaaaataac cagaatagga atgccaagac gggaagaaaa tgaactaagt
    18721 aatatactgc tggtgacaag gatggatcct aagatgaaaa ggctaattat tgttgtggca
    18781 tccaacggtc ggttactcct gattacgctg tctcttatat aaaccctacc atattagcgg
    18841 cagagacagc gttttactta gccctgaagc gtgatttttt tacattttaa gaacaggatg
    18901 accgctaatc gtcaactgag tgccttcccg ggtgttattc agaatggcat gtgcgcccaa
    18961 cggcagcgta accgtgcgtt gttcatgacc aaaatcgagg ccggtaatga gtggaatcga
    19021 caggcgggaa cgcaaaaacg cgtacactga ctccaggttg taacctgcgt cataatcatt
    19081 gggcgtgctg ccgctaaagc taccgagaat aatcgccttc tgacgtggca aaattcccgc
    19141 atgatagagc tgcaacagca tacgttcgac ccggaaaggg tgctcgttaa tatcttccag
    19201 caccagaata ccgttctcaa tttttggcat ccacggtgta ccgatcagtg aaatcagcat
    19261 cgcaagattg cctccccaca acgtgccttc ggcccgacat gtcgggcctt ccccttgcca
    19321 ttcaatggtg aaggtttcat tgcgtaacgc cagccagaaa tggtgctcgg taaaggcgtt
    19381 cagttcatcc gcgccaaagt ttgctaccag catggggccg ctaaaggtga tgacattgcc
    19441 atgcgccaga agaccgcact gaatggcggt aaaatcgcta tgtccgcaaa tgagcaacgg
    19501 gtcatgttgt tggcgagcca ccagtgcctg ccagtcaata tctgccagta aacgactggc
    19561 accgtaaccg ccgcgtacag ccagcacgat ggtgttgggg gtagtcagtc tggcaaggga
    19621 attgagatct tccagacgct ctgtttccgt acccgcaaaa cgctcacaac gacgggcaat
    19681 aacctcgacg ttattgactt gatgccccgc gtcggtaagg cgctggatac cacgcagcgc
    19741 ggcgtgctgt ttaatgcagt aacccgatgg ggcaattaag tgaaacagag acatggcaat
    19801 tccttgctga caacagaaac gaaatgtata tcatgccgct taggtgtgcc gttgtcacct
    19861 caacggcgat tccaggctat aaggatagaa gaagtgaaat tgagatggtt tgcctttttg
    19921 attgtgttat tagcgggttg ttcatcaaag catgactata cgaacccgcc gtggaacgcg
    19981 aaagttccgg tgcaacgtgc gatgcagtgg atgccaataa gccagaaagc cggtgcagcc
    20041 tggggcgtcg atccacaatt gatcacggcg attatcgcta tcgaatcggg tggtaatccg
    20101 aacgcggtga gtaaatcgaa tgccattggt ttgatgcagt taaaagcttc aacctccgga
    20161 cgtgatgttt atcgccgtat gggctggagt ggtgagccga cgaccagcga gctgaaaaat
    20221 ccagagcgta atatttcaat gggggcggct tacctgaata ttctggaaac cggcccgctg
    20281 gcaggcattg aagatccgaa ggtactgcaa tatgcgctgg tggtgtcata cgctaacggg
    20341 gcaggtgcgc tgctacggac tttctcgtca gatcggaaaa aggcgatcag caaaatcaac
    20401 gatttggatg ctgacgagtt cctcgaacac gtagcgcgaa atcaccctgc gccgcaggct
    20461 ccgcgctata tctacaaact tgagcaggca ctggacgcga tgtaaatcag tcgcgcactt
    20521 tgtccgcttt ttcccgggct tctcgctcga gagagaaaat aatccgctgt aattgccgct
    20581 ccaccgtcgg gctgacgtta agaaaacgga agctcagacg gggagtggtg atggtttcat
    20641 tcttgccatc aatcactttg cgctcgctga tggagattaa ctgggcgtca aagtgaaaaa
    20701 caccccattg cctcatgttg acttcaatct gagcgaagcg catgccttct tgtaattcgg
    20761 caggctttgc tgtttccagt aatgcgccca tgccgcctaa cgacaaatca tacaggcgga
    20821 aacgtaacgt actgttatcc gccagtttgg tctggcaaaa ataaggcgga tggagtgggg
    20881 cggagatgcg gaaatatcgg cgtcgttgta caaaccataa ggtgggaggc ggtacggtga
    20941 taaatgccgg aagctgcaag tattcactct gctgtagttg ttcaacagta aactcgactt
    21001 tcgcaccctg agtttcggcg gtaatggtaa tgtgctgtgc ctttagcacg gcgatgttgt
    21061 cttcggcttg actgccgaaa tccagcacca gtttatccgg ggttattgcc agtattttgc
    21121 tgatcagctg cccgccattc caactgagac gcaaaggaat tgcggctttg tgcaaatcgc
    21181 gtaacacgcc caggacggct aacggatttt gtttcaggaa ctgctcatgg taatgactca
    21241 cgcggaaaaa ctcctgactg ataaactgtc tttggtttat cggtcacagt taacaaaact
    21301 taatacaaat gcgtgaatat tttttacatg ttgttcttaa atcagccgac atacgcccag
    21361 catcgatccc gcccttgcct atacttagag cgttgacgta agcatttctt gcgtccgatt
    21421 catcgaaacg agggcctgaa catgggaatt attgcctgga ttatttttgg cctgatagcc
    21481 ggcattatcg ccaagctaat catgccgggg cgtgatggtg gtggattttt cctgacctgt
    21541 attctcggga tagtcggtgc ggtggtcggc ggctggctgg cgaccatgtt tggcattggc
    21601 ggctccatca gtggttttaa tctgcacagc ttcctggtgg cggtggtggg agctattctc
    21661 gttctgggca tattccgcct cctgcgaaga gaataagatt ttcataaggc ggatagcgat
    21721 acagatgccg ctatccgctt tcacatcaga acgtatactc gacacctgct ttacgggtga
    21781 aaaaaatcaa tatagcactt cgatatccga taaagtcggt tcccggtaac aacaacagaa
    21841 tcctttttta attattgttt cgttgttttt ggtgtgattc agaaaataaa tattttagac
    21901 agtagccagt caggcgttaa aaatggggcg gatagtgagg cggttttggc tgcgcttatt
    21961 gaacatttag aattgatcaa tccttctggc agattaagcc aaaatacccg ttcggcaatg
    22021 cttcagttac gcgaagagga atggtctgaa ttcttttttt ggttgctaaa ttctctggag
    22081 tgtctggatt atgtgattat taatttaact ccagaaagca aaaaaacgtt gatgagtgaa
    22141 caccgtaaca atattcaggt agcaattgat gctttatata gccaacgtcg gcgtaaatcg
    22201 ccgggtgatg aaagtgaaac acttacccgt agaaatgacg ctatcttcgg caaccacgta
    22261 tggcaaacat ttgcgcaata cttcccgcca ggacttgaaa aaccgtctgt gtagaaaaaa
    22321 ccatagctat ggtgttatct gccgacaacg ccatccggag aagccgaatg acgttgtcgg
    22381 agtaagcgct ggttaaggtg tgggttgtgc ctctttggtt gagggttgcg tcgttgctga
    22441 cttaacggtc ggacgcgtcg ccggaacatt gtcacacggt tgctctttcg ggcagatcaa
    22501 atccagcatt ttcagcgtca cgccattggt ccagccaaag ccatcctgta atggatattc
    22561 gccaccgccg ccccccgttc cggtggtgct gacatcatat ttttccacca gctttttctc
    22621 ccggtcatag gtgtgctgaa cattggtcag gaagtgccag ctaatgtcca tcgccacctc
    22681 tttttgcccg tagttttgta atccttctgt cgcgacccac tgtaacggtg cccagccatt
    22741 tggcgcatcc cattgttgcc cacttttcac cgacgtggtg ttcaggccgc cgggttgcag
    22801 cagatgtgtt ttcgtcgccg tcgccatttt gttggcgcga tctttcgctg ccgcattgac
    22861 gtacagcggg aacagggcgg ccgcggttaa ctgattgcgc actttatgac ttttcaggtc
    22921 gtaatcggca taccagcctt gttgatcgtt ccacaggtat ttttcgatcc ctttttgacg
    22981 ggcatttgcc agcgtttcgt actggtttgc catcgcgtta tctccggcag ctttgctggc
    23041 gcgggcgagg attttttcca ttttaaacat caggctgttc agatcgaccg gtacgatgct
    23101 ggtggtgcgt aaggtattta actgctgcgg gttgtccatc cagcgcgagc tgaaatccca
    23161 gccagacgca gcggcagagc gcaggtcgcg gtaaatttca gtggcaggtc gattcggatt
    23221 gcttttggcg gtggcaatat cttccaccca tgactctggt cgtggcgtat cgcgatcgtc
    23281 ccagtagcgg ttgagaaggg taccatcctg aagttttaca acgcgttttt cctgttgtcc
    23341 ggcttgcagg ttttcaacac cgtccatcca gtaagcatat tctttttgca tttgcggcag
    23401 gtattgcttc aacgcggcat cgccttcatg ctgcgccagt aactctacca tcagggcaaa
    23461 gaagggcggt tgcgagcggc ttaaatagta actgcggttg ccgttgggaa tatgaccgtg
    23521 agtgtctatt tcatgagcaa aattggccac catatccgcg actttatccc agtgaccgct
    23581 ttcggcaagt cctaacatgg tgaagtaact gtcccagtaa tatacctcgc gaaaacgtcc
    23641 gcccggcacg acataaggtt ccggcagcgg taacagagaa tcccattttt cggtgttttc
    23701 ggtagaacgc gttaataccg gccaaagtcc gtcaatatgt tcgcgcagtg actgtccctc
    23761 tggcggaaca tatttctcgc cctctttcgg cagggtgaaa ttgacgttaa cgaaatggcg
    23821 cagatcaaat ccgctctggt tttgctgcat ccgataatca gcaaggatca tcagcggatc
    23881 gctgttcggc acggcatcgg caaaggtttt ttggtccgga aaaagtttgg cgttttgcac
    23941 atcattaaac agcggcccta ataaaatatc aggcggctgt ggtgttaccg gtgtttcttc
    24001 tgcctgcacc gatagcgcag cgaaacacaa aaagatgcag gctggaatta acgccatttt
    24061 ttgcgggcga gaaggtgcgg gggatttcat caatcattct cctttggcga aaccgaataa
    24121 aacggtattc aattatcaga aaaccttagt tcaggatcgc actagctgca tgatcgaaat
    24181 ctcattctgc gaactgtcag gcattttaga aaagggaatt aaagggataa ataacggcga
    24241 aatagagtga tcaaataacg tctgtttatt agccagccag cggcgttggg ttcgtgatta
    24301 gcgaagaaaa atcatcataa attggtgaaa aatataacag gtatttaaaa atcattagtg
    24361 ctgggtaaat tgccggatga catcagaacg atgccatccg aacggtggct taaccctgac
    24421 ggttgaaacg ttgcgtttta acgtccagcg ttagcgtttc ttctggttgt atcgcataca
    24481 gtttctcacc ctgctggcaa atccagccaa tccccagttc acgggcgatt agggcgctgt
    24541 gggataccgg acttccggcg ctaaggcaga taccttttac aaccgccgga tccagttgca
    24601 gtactgtgga aggataaatg ttctccgcca gtagaatagt tggcgagtta aactgcggga
    24661 gttcttcttt cgtttgggtc aggtggacca gggtgcgatg cagaagatcg tccacatcaa
    24721 tatagcgagc ttgtagatat tcatcatcca gttgctggta ttgctggcta agttctttaa
    24781 gaacttgctg ccaggcatat tctgccgtgc aatgttcatg ctgaaggagt tcgcttgccg
    24841 ccgccagcag ttccggatca tctaacagtg tatggtgacc agaaaagatt gcggcaatat
    24901 cgtcaagccc gctggcttct gctttcgctg ttaacgtcat cagatctaat aacgtgaagt
    24961 caatagcctg gcgtaatcgt tcttgttctt cttccacggt cagggttgat tttgcctgta
    25021 ccgtacataa aactggttga taataaaagg ctttacccga aacaggcgga acgggacgca
    25081 gagtaggtgg agcgacttcc tccgtttcac caaagttatc ttcagccagc tgacggaaag
    25141 cgatcagtgc ctcttcagct tctggccctt tcgcaatcag gcgcagcgta tcgttatagc
    25201 gaacttgtag taacgcaatc tggttaatac tctctggtgt gacgcatttg ccgttttttt
    25261 ccagcaacat atcggcatta aatgtcgata aggtataaac cagccgggag gccggacgta
    25321 catgcaggcc gttacggttt tttatgacca ccgccagaga acgggcttct tcatcgtacg
    25381 caggacatgt gtcagagatt tcagtgtcgg aggacggtaa acccagttgt tcacgtttgg
    25441 cttccagcgc atgcatagcg tcaaagataa ctttgtcgat atccgctccc gaggccgcgc
    25501 tgaccgttgc tgccagtgta ccttcgacca acggcgcagc acacaaacgt acttttgcgg
    25561 cgatctcggg agccagcaat tccagcgcag tttcagcact caataatgcg ctacccatat
    25621 ccatcatgac cagcacatgg tcggcatcag caacagattc gatggcctcc atcactttga
    25681 cggcatcggt accaatggga ttttgtggat cgtcaattcc cgcggcaatg gcgattttac
    25741 aactatcact cattaacatc tgacgggcta attcaccgac accttctccc agtcggctgc
    25801 tatgtgaaac tatgaccagg tttaccatca ccaattcctt actcttttgc ggctaacgcc
    25861 aacatttgca tcataaacat caccgaggtc gcgccgggat cctggtgacc aatactgcgt
    25921 tcaccgagat aactggcgcg gcctttgcgg gcttgcatcg taatcgtact ttgtgcagcg
    25981 gattcggcga tgctactggc agcttcgagc gccgccggaa cagagagatt ttgctcgctg
    26041 gactgacgta acgattccac caccggcacc cacacatcac acatggtttt atcgccaggt
    26101 tcggctttcc cgcgactgat tacgccgtcc gcgccatcgc ggaacatctg ataaagctct
    26161 tccagcgtca ggctttgccg tgcctgggtc gcctgtgcgg cgcggataaa gaaggtaccg
    26221 aacagcggac cactggcacc accgacgctg gaaagcagcg tcataccggt attcttgaga
    26281 atgaaaccga tatctttatc tgcgatagca gggagttttt ccactacttt gctaaagcct
    26341 cggttcatat ttagcccgtg gtcagcgtcg ccaatttcgc gatccagtcc ggtaagatac
    26401 tcgctctcgg tgctgaaaat atcgccacaa cgagtgagcc agttaacaat ttgagttctg
    26461 ctcagtgaca ttgctttctc cttatttacc ccagttaagg gccggggtgt ggaccggggc
    26521 gtcccagagt gccagcgttt cgtcatcaac tttcagtaag gtgattgaga aaccggtcat
    26581 atccagtgag gtgcagtacg cgccaattaa attacgttcg atagtcaatc ccgcttgctg
    26641 gcaacgtgtg gtcaggcggt tatagacgcc gtacagctca gaaagcggag ttgcgccaag
    26701 attgttaacc agcgcaatca cccgatcgcc agactggagc ggttgtttgg tttgttgttc
    26761 ttcctgccaa ctgccttgtt gataatccca gaaacgcaaa gtgcgatggt atgagccatt
    26821 ttccagcagg gtgtcgaaca tttcatcgac ggtttgatca agggaagaga aggagcggcg
    26881 gtcaataccc ggctcaccat gaatgccgac gccaaactcc atctcattat ccgccagggt
    26941 aaaagaaggt ttgcccgcgg caggaacggt acaggcaccg agagcgatac ctattgagtg
    27001 gccttgatta ttcaacttac gccccagttc cgcacaggcg tccagtgagt cgccacgctc
    27061 ggccgctgcg cctacgagtt tttcaattaa tacggtgttg gcaacgccgc gtcgcccggc
    27121 ggtataaaga ctgtctttta ctgcaacgtc gtcatcaatg accacagtgg tcacgtttac
    27181 gccgctatcg tgcagtaact cggttgctgt ttcgaagtta agaatatcgc cggtgtaatt
    27241 tttgataatc aacagtacac cttcgccgcc atcaacttgc atggcgcatt caaagatttt
    27301 atcgggcgtc ggtgaggtga aaatttcgcc cggacaggcc cccgaaagca tcccctgacc
    27361 gatataacca cagtgcatcg gctcgtgtcc gctgccgcca cccgacagca gggcgacttt
    27421 tcctgtaacg ggggcatcag ctcgggtgac atacaccgga tcctgatgca gtgtcagcga
    27481 tggatgtgct ttcgccagtc ctgccagttg ttcgtccagt acgtcttgca catcattgat
    27541 caattttttc attattttgc tccagcaatt acggtagggc atggatgatg ttcaacgaca
    27601 cggcgaccac tgactgccga tgaatccatt gtgcatcagg caagggaaaa gaaaattagc
    27661 ccaaattatg tttcatagtg aaacatatgc tttaatgaat gttccatatt gaaactttta
    27721 cgtgtattaa tacttaaaat tgcgagccgg aacacctttt gtcataaggg atgcgggata
    27781 tgagtggcgc ttttaacaac gatggtcggg gcatatctcc cttaattgca acctcctggg
    27841 agagatgcaa taaactgatg aaacgggaga catggaacgt accacatcag gcccagggcg
    27901 tgacatttgc ttctatttat cggcgtaaga aagcgatgct gacgctcggg caggctgcgc
    27961 tggaagatgc ctgggaatat atggcaccgc gagagtgtgc gctgtttatc ctcgatgaaa
    28021 ccgcctgcat tctcagccgt aatggcgatc cgcaaacctt gcagcagcta agtgcactgg
    28081 gattcaatga cggcacgtat tgcgccgagg gaattattgg tacttgtgcg ctatcgttag
    28141 cggctatctc tggtcaggcc gtgaaaacga tggccgatca acatttcaaa caggcactct
    28201 ggaactgggc cttttgtgca acgccgttgt ttgacagcaa gggccgattg acgggaacaa
    28261 tagcgctggc gtgtccggtt gagcaaacta ccgcagctga tttgccgttg acgttggcaa
    28321 tcgcccgtga ggtcggaaat ttactgctga cggacagttt gctcgctgaa actaaccgtc
    28381 atttaaatca acttaatgcc ctgttagaaa gtatggatga tggcgtgatt agctgggacg
    28441 agcagggtaa tttgcaattt atcaatgccc aggcggcgcg ggtcttgcgc cttgacgcga
    28501 cggcaagtca gggaagggca atcactgaac tcttaacgtt acccgccgta ttgcaacaag
    28561 caataaaaca ggcacatccg ctcaaacacg tagaagcaac ctttgaaagt cagcaccagt
    28621 ttattgatgc ggtgataacc cttaaaccga taatagaaac gcagggaacc agttttattt
    28681 tgttgctcca tcctgtggaa cagatgcggc agttgatgac cagtcaatta ggaaaagtca
    28741 gccatacctt cgctcatatg ccacaggacg atccgcaaac ccgccgcttg attcattttg
    28801 gtcgccaggc ggcgcgcagt agctttcctg tcctgctttg tggagaagag ggcgtgggca
    28861 aggcactgct aagtcaggca attcataatg aaagcgagcg tgctgcaggt ccttatatcg
    28921 ccgtcaattg tgagttatat ggtgatgcgg cgctggcgga agaatttatt ggcggcgatc
    28981 gcacggacaa tgaaaatggc cgtctgagtc ggctggaact ggcgcacggt ggcacgctgt
    29041 ttcttgaaaa gattgaatat ctggcggtgg agttacagtc tgctttgctt caggttatca
    29101 agcagggtgt tatcacgcga ctggatgcgc ggcgtttaat accaattgat gtcaaagtga
    29161 ttgccacaac gaccgcggac ctcgcaatgc tggtggaaca aaatcgtttt agtcgccagc
    29221 tgtattacgc gctgcatgca tttgaaatta ccatcccgcc tctgcgtatg cggcgtggca
    29281 gcattccggc tctggtgaat aacaaattac gcagtcttga aaaacgcttc tctacgcggc
    29341 tgaaaattga tgacgatgcc ctcgctcgcc tggtttcttg tgcatggcct ggcaacgatt
    29401 ttgaacttta cagcgtcatc gagaatcttg ctctgagtag tgataatggg cgcattcgcg
    29461 tcagtgattt gccggaacat ttgtttaccg agcaggcgac agatgatgtc agcgccacac
    29521 gcctttccac cagtctgtca tttgcggaag ttgaaaaaga ggcaattatt aacgcagccc
    29581 aggtcacagg cggtcgcatt caggaaatgt cggttttact tggaatcggc cgcactacgc
    29641 tgtggcggaa aatgaagcaa catggcattg atgcagggca gtttaagcgc cgggtatgaa
    29701 agacagagac gatttctgat acatcagagt gatctatatt tcattccggc gcacgctaac
    29761 atttttcagc atcgtttaag ggcttgtcta ccccgtactt aaaagctgaa gtgatatcct
    29821 ccgttgactt gcttctgatc aaagaggtta ccctgcgtgt aatccgcttc gaggtagaat
    29881 gtgtgctgtt tgttatactg tgcactgact cccacgccgt tattccagcc attacctttg
    29941 aaactgtact tctcccggga gttgttcaac agatattctg tatcccctga aaactcgcgg
    30001 atagcgccag tcttcacata catattcagc tggctgtagc ctgcggtgat gtcatatccc
    30061 agtatcatac tggcacgccc cagcagcgat tcgtagtgat tcagatgtat attgaggcca
    30121 ttactcgcct tcatagccat ctcattctgg tggctgtatg tgagctgggt ttgcggctct
    30181 atatagaacc cataaccagt aggggtcagg ttgaacctct gcccggcttc cagggagatg
    30241 ctcaatccat tcgcagtgcc gttggcgtta acgccgttgt tctgactgtc ccgtacgtgg
    30301 aaactatttt tctggcgcga tgcttttata acgagatcgc tgtaaaaacc gttttgtgcc
    30361 atgtaactgg cgtacattcc catgtagtct gaacgtgcgg taccgtcgcc tccgctatag
    30421 tccggcgatg catgtgttga gccaatatac agaccgacat acaacggcat tacatcagag
    30481 agacgtttat ccccaccaaa ctggataccg ctgtaactca tgtcaaagcc gctcagtttg
    30541 ccactggcaa aggagtccag gcttccccca taactgcgca accagatatt accgtcttta
    30601 ctctgatttc gcaggtcacc catccgttgc atcagcgtac ggttttcaac atagttcaat
    30661 aagtaaccga cattgagata attgccgcca gcatctgcgg tcgtggtggg tttaggtgtg
    30721 ggagtggggg taggcgtagg atcgggttca ggcgtaggat cggggttgac tatgggaggc
    30781 tgagcgggag ccggtgttgg ttcaggatta ggagtcggtt ccggaactgt cccggaagcg
    30841 taaagctccc agttagtgcc atttttacgc acatcgtaca gatatccccc caactcaacc
    30901 tgagaagacg cgctgaacga ggccgcaccg tcagtggttt ttaccactgt cagaacttca
    30961 tttcccgttg tggcctcgct gccctggttg cggatagcca atacgtgatt accagcacta
    31021 ctcccgctga tattcaataa atccccttta ttattaacgc cattaccctc accaacaaca
    31081 tcagcacgca taataaaggt actgttaccg ctcaggttct ctacgtttaa tgtggtaaat
    31141 gtgccggcag ttgacccgtg gctggcaaaa tcgacagttg aatggctcag cgccagcgtg
    31201 tcgagattag aattacttgt tacgttccag acgctgttat tcattgcaac gtccagtttc
    31261 ccgtcattga cattatcgct aagggaggaa cctgtccaaa ccgacccagg gtgcatatcc
    31321 agattgatta gcccaccttt ggagagaacg ctaccgttga taagcatacg acccgaagcg
    31381 ttgatgcggc tggcggcata accatcgtca tgttgcgttg cgatggccat ctggtcgggt
    31441 gtactcatat caatgaccag atcgctcgtg aggtcgatct ggctgttggt catggcataa
    31501 atccccctgg ctcctgccgc gccggtgata gccaaactgt ctccggttat tctaccgccg
    31561 ctgagcgccc acaaacccag cgccagactg ccattacgat caaccgtaat atccgtattt
    31621 tgcatgttga taacagccgt tgccgtctgg gccgaggcac cataagaacc gccggaaaag
    31681 atgctgtttc gttgcgctgc cgtgccagta aaattgatta tcgcgtctga accactggtg
    31741 acgaggccac cgccctgcgc ggaagaaata tggctatctg caccgatagt ggttgtacca
    31801 ccacgaactt cgacgccatt ggccgcggct ccagttacat caacagtgag tgcattcgcg
    31861 ctaacctggc caaagctcca gaggccgtga gcattatcgc cattggtttt aatggtactg
    31921 tttgttccga gatcgacaac agagtttttc tgtacgttta tccccatggc gctgtagccc
    31981 tgaacatcga ttgtcaggtc tgtagccgta aaacgcgcag caccattggc gttattgccg
    32041 ttgagaccac cgatataaac acctgtactt ccatcggtcg tgattttact tccgcttcca
    32101 agatcgacac tggtgccata gtcattgatg gttaggccta taccgttcga gttttcaatg
    32161 gtgaattgag tggctgttag tgttgaacta tgcccaataa tgatgccgtc atcgttactc
    32221 ttaatggtgc tgcctgtgcc taagtcagca tgggtatagt caccaattaa gttaatgccg
    32281 atggcagagg tttgaccaac aacatctact gtgagtcggt tagctgtcag ggagctgttt
    32341 ttgccctgaa taatgattcc ttttgcatat ccagaggcgt cgttgacatt gacggtgaca
    32401 tcattaccca ggttgatatt gccaggcgtg tttcctgctg gggtcattac accatagagg
    32461 tttcctgatg gatcggcggt atctcctgta attctgtcac catcattaat agtaatattg
    32521 gtgccatatt gacttcctgt atcagcagct atcagcaggg gggaaaagat aattgcattc
    32581 aaaccaatag cgccaaattt tatcagttga attgagggcg aacggatttt taattcagcg
    32641 agacgatctg ctttggtatt cccattgtgt tgtttgatgc ccataccact cctatatagt
    32701 acccagacta tgggtctatg gattttgtca ttttgtttaa atcaatgaat aatatcctct
    32761 tatcatcatg atatttgtat ggtgcaatca tgatgagctt ctcagagaat aattctctat
    32821 taccgggcaa aatcaggaac acaggaatta atatatattt aacatccccc ttacaaaggt
    32881 gagtttatat cgagttattc cctaagttg
//
LOCUS       DAJOSY010000046        32657 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJOSY010000046 DAJOSY010000000
VERSION     DAJOSY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.046"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(204..2837)
                     /gene="mngB"
                     /locus_tag="OHN27_002221"
     CDS             complement(204..2837)
                     /gene="mngB"
                     /locus_tag="OHN27_002221"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCQ2286656.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDVMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(2855..4771)
                     /gene="mngA"
                     /locus_tag="OHN27_002222"
     CDS             complement(2855..4771)
                     /gene="mngA"
                     /locus_tag="OHN27_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCQ2286657.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHVLTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            4946..5668
                     /gene="mngR"
                     /locus_tag="OHN27_002223"
     CDS             4946..5668
                     /gene="mngR"
                     /locus_tag="OHN27_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCQ2286658.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIERSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5772..6641)
                     /gene="sucD"
                     /locus_tag="OHN27_002224"
     CDS             complement(5772..6641)
                     /gene="sucD"
                     /locus_tag="OHN27_002224"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCQ2286659.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6641..7807)
                     /gene="sucC"
                     /locus_tag="OHN27_002225"
     CDS             complement(6641..7807)
                     /gene="sucC"
                     /locus_tag="OHN27_002225"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCQ2286660.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDQREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(8173..9390)
                     /gene="odhB"
                     /locus_tag="OHN27_002226"
     CDS             complement(8173..9390)
                     /gene="odhB"
                     /locus_tag="OHN27_002226"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCQ2286661.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(9405..12206)
                     /gene="sucA"
                     /locus_tag="OHN27_002227"
     CDS             complement(9405..12206)
                     /gene="sucA"
                     /locus_tag="OHN27_002227"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCQ2286662.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12508..13224)
                     /gene="sdhB"
                     /locus_tag="OHN27_002228"
     CDS             complement(12508..13224)
                     /gene="sdhB"
                     /locus_tag="OHN27_002228"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCQ2286663.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(13240..15006)
                     /gene="sdhA"
                     /locus_tag="OHN27_002229"
     CDS             complement(13240..15006)
                     /gene="sdhA"
                     /locus_tag="OHN27_002229"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCQ2286664.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(15006..15353)
                     /gene="sdhD"
                     /locus_tag="OHN27_002230"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(15006..15353)
                     /gene="sdhD"
                     /locus_tag="OHN27_002230"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCQ2286665.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15347..15751)
                     /gene="sdhC"
                     /locus_tag="OHN27_002231"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15347..15751)
                     /gene="sdhC"
                     /locus_tag="OHN27_002231"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCQ2286666.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            16132..16239
                     /locus_tag="OHN27_002232"
     CDS             16132..16239
                     /locus_tag="OHN27_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286667.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16445..17728
                     /gene="gltA"
                     /locus_tag="OHN27_002233"
     CDS             16445..17728
                     /gene="gltA"
                     /locus_tag="OHN27_002233"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCQ2286668.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            18117..18683
                     /locus_tag="OHN27_002234"
     CDS             18117..18683
                     /locus_tag="OHN27_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2286669.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            18734..21193
                     /locus_tag="OHN27_002235"
     CDS             18734..21193
                     /locus_tag="OHN27_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2286670.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGIL
                     MDYNLFASSYRPQDGSSSTNLNAYGTTGINAGAWRLRSDYQLNQTDSDDNHEQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDERMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVTENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            21208..21939
                     /locus_tag="OHN27_002236"
     CDS             21208..21939
                     /locus_tag="OHN27_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCQ2286671.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGSYSGSQFYLGYMD
                     DYGALRMTMLNCSGQCHLQAVEAKK"
     gene            21936..22997
                     /locus_tag="OHN27_002237"
     CDS             21936..22997
                     /locus_tag="OHN27_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2286672.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            23149..24195
                     /locus_tag="OHN27_002238"
     CDS             23149..24195
                     /locus_tag="OHN27_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCQ2286673.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(24192..24983)
                     /gene="nei"
                     /locus_tag="OHN27_002239"
     CDS             complement(24192..24983)
                     /gene="nei"
                     /locus_tag="OHN27_002239"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCQ2286674.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGIWRVVDTGEEPQTTRVLRVKLQTVDKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(25019..25753)
                     /gene="pxpA"
                     /locus_tag="OHN27_002240"
     CDS             complement(25019..25753)
                     /gene="pxpA"
                     /locus_tag="OHN27_002240"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCQ2286675.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(25743..26675)
                     /gene="pxpC"
                     /locus_tag="OHN27_002241"
     CDS             complement(25743..26675)
                     /gene="pxpC"
                     /locus_tag="OHN27_002241"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCQ2286676.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFQQLAWRLHNEN"
     gene            complement(26669..27325)
                     /gene="pxpB"
                     /locus_tag="OHN27_002242"
     CDS             complement(26669..27325)
                     /gene="pxpB"
                     /locus_tag="OHN27_002242"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCQ2286677.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(27348..28091)
                     /gene="ybgI"
                     /locus_tag="OHN27_002243"
     CDS             complement(27348..28091)
                     /gene="ybgI"
                     /locus_tag="OHN27_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCQ2286678.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            28362..29843
                     /gene="dtpD"
                     /locus_tag="OHN27_002244"
     CDS             28362..29843
                     /gene="dtpD"
                     /locus_tag="OHN27_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCQ2286679.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITILFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(29993..31411)
                     /gene="phrB"
                     /locus_tag="OHN27_002245"
     CDS             complement(29993..31411)
                     /gene="phrB"
                     /locus_tag="OHN27_002245"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCQ2286680.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDHEGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(31408..31917)
                     /locus_tag="OHN27_002246"
     CDS             complement(31408..31917)
                     /locus_tag="OHN27_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCQ2286681.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(32068..32657)
                     /locus_tag="OHN27_002247"
                     /pseudo
     CDS             complement(32068..32657)
                     /locus_tag="OHN27_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         7801 a         8794 c         8031 g         8031 t
ORIGIN      
        1 ccacatatac gtggcttgag agtgaagata aacgcaaaat tacctgatgc gctacgcttg
       61 ccagtgtcta catcatctct gcaatatatt gaatttgcct gcttttgtag accgaataaa
      121 gcgttcatgc cgtatccggc atgaacaaag cgccctttgt caacaatctg gccgcgcata
      181 tgtggcttcg gtttgattgc tattcaggca agccggtaac tgaacgtccg tgactggccg
      241 ggtaaaaaag gccccgatag atttgaacct tgattttcct cggtggtaat gtgttcatcc
      301 atcatcgttt ctgagcaaga aatcacctcg cgactgaaag caacagtcgc atcacaggtt
      361 gctgattcag ccggattaaa cagccgtaaa atcacttctt gtcggtcttc agctttctta
      421 agtgcgctta tcaggcatcc cactgggggc attttcaaca aactataact ttccggcacg
      481 ttgaatccgg ctttgttgag cttcatcaca tcccatggga ttttgttgta acactgtact
      541 ggagtcagcc atgctcgcgc ctgctgagct acaccagcgg cggttggcgt accggtataa
      601 ctcaataaac ttaggcgaca agaaagcaga ccacgtagtt gtgagtccgg gactggcatt
      661 ttaattcccg aaggccgccc aggccttaaa agcagatctt ctttgcccag taagcccacg
      721 ccacgcagca acgtaatggc aaaggttttc ttctcttcac cgatgacttc aaattcacgt
      781 aacccttcgc taaagacagc catgccgtta cgcccttcct gtaaggcaac atagttgagc
      841 atattccata ccggaaccgg cgcttctttc cagccttctt gctgccagtt gttcattgca
      901 ctgtcgttca cggggcgcgt tagcgaacca aactgcgtat ctgccagaac actgtcggtg
      961 ttaaaaggtg tagggaccag gacacgaagg cgatgatcgt cagcctggtt atcaagattg
     1021 atatccacat caatacgcct gctgttatga ctaagagtga caaccaacac cacccctact
     1081 ctgccagtgg attgccgggc gctgcgttct gacaaattga gcggcactgc catgtcatag
     1141 cggataacag ccctgctctg ccaggcttca tgaataatat cgcattgcgg tttcgcgttc
     1201 gctgcggtaa ttacccactc ttcttttgcg ggtgaatagt catattcatc accatcatca
     1261 gagctttctt caatttgcaa tacccggtca tagcgcacac cgctgtcttt atctaccagt
     1321 tgcagagaac catcctcatt gagcgcaatt tgccagaaag cattttccag tatcccttca
     1381 gcgtcacttt ttgccgcaat tacgttgcca ggctgattcg cttcgatata aagcgtgcga
     1441 tagcccatag aagggacaat ctggttgatc tgtatatcaa actccataaa gggatcgtaa
     1501 ttaccgtaat gaactatttg ccgatcgatt aggcctggat cgatctcacg ggcatggcga
     1561 ataaaatacg gtacaggctg accgcgatcg tcccgcaaat taaactggct ggcgcgcagc
     1621 cgcacagtgg tgttgataac ttcttcacgc ggccagggca tcaggttaaa caggacgagt
     1681 ttgtcggcgt cgctctgcgg catgttgtcg gcaattttgc gcatgtagaa acgaatcaga
     1741 ttatccgcca tgtcttcagc cagttcgaag cgggcgacga tttcgcgatg aactttgtca
     1801 ctacagcagc agccgatact gtcgtgggca tgatttttta agatctcttt ccacattttc
     1861 tccagcaagc cgtggtggta ttcaaaaccc aacgtccagg ccagtgttgc cagcggttcc
     1921 agcagattaa caatcttatt ttcaatacgc gcgtgggcaa ttttgatatc catacgcgta
     1981 gaaccgatgg tgcgatgcac gcgcatatat ttgccatcaa taaattcccc tttcagggtt
     2041 gccagattat ctcgctgcgc ttcgatcttc tcaaatacct cttcaaagcg gctcatcaca
     2101 aacttacgtt gagggtagat ctcacgtagc ttatccatca cttcgaagat attttgctgc
     2161 aatggcatct ggtcatgccc attcggcagc aaaatctctt tggttacaga cgctttttcc
     2221 agcacgtcaa aataactgtc gaggcgttta cgtaatccgt tttcgtcggc aggtaagtac
     2281 ttaccgatgg cgtagcccag cggcagcacc tgcgccgtca cttcgctacc gtcactgctt
     2341 tgccacaaaa actcggtttt atcagtaccg tggcgctccg aacatccgcg ccagaacatg
     2401 gtgcgggtaa tgccaaatcc attgtagata tgcggcagtt gcccggacat gccaaaggaa
     2461 tctggtaaat aacctatttt catcggctcg ccaaacgcga ggcagtcacg cattccgtac
     2521 atcagattac ggacgatgga ttccgcagaa acaatcgtgg tatcggtctg ggtataccag
     2581 gggccgataa tcaacttgcc ggcttctacc tgtttcttca cacggtcttt gttttccggt
     2641 ttcaccgcga aataatcttc gaggatcgcc gtttgcccgt cgagtacgta atatttgtat
     2701 tcgttgtcct gttccagtcg gcacaggatc tcttccatat tattgaccag cagaatacgt
     2761 gactcttcgg tggtgaaata ccactctcga tcccagtgca tatgcggggt gatgtgaacg
     2821 cgagatactg ctttcatcgt cgtttccttt ttgtttatgg cattacgcca tcagtcagat
     2881 agttgccatg cttaaccgcg tgacgccgcc acatcaggag aattgcagtc gagattgcag
     2941 cccccaccaa tgccgcgcca aaccagccaa ttgccgccat aacaccgccc gcgccattat
     3001 catgaagtaa aaagagcgag aaaatgccgg caccgggtgt cgaaaggccg atattcatcg
     3061 caccgacaat agcgcccgtt accatagagc ccagcacaaa cgaaccaata acccgcagcg
     3121 gatcttcaat cgccatcggg atcgcccctt cggtaatacc tgccagccct aacagccagg
     3181 tggatttccc ggtctcaatt tcaaactctt taaacaggcg cggtgcgagc atcgtggaag
     3241 cggttacggt aaatgccgaa accattttga cggaggcgaa aatggcatac gggccgtaaa
     3301 cgccgttcgc cattgcgccc aggcagaatg cataagcggc tttattcact ggccctccaa
     3361 ggtcaaagga acacataaaa ccgagaatgg cacccagcaa cagcgcgtta cttcctgaca
     3421 gaccgttcag ccaggcggta agcgagttat tgatccaggc gacaggttcc cccaccacaa
     3481 acagcatcag actgcccgct cccaacgtac cgagcaccgg gtagagataa aaagtcagga
     3541 atccattgaa tttactgcta agacgcaagt gatttttcac ccagcgcatc aagtaaccgg
     3601 ctatcaatcc gccaacgacc gcgccgagaa acccggagcc gatcatgttg gcggcaagtc
     3661 cagccgcaaa gcctggcgct aacgccggtt tatctgccag agaataggcg gtataggccg
     3721 cgagcaccgg taccatcaaa attccgagca gcccgccgcc cagcttgcgg tacatccaca
     3781 gccaggagtt ttcttcatta aacagatctt gtagcccgaa gatttgcgac agtaataccg
     3841 cgaccgccag caccgtgccc cccgcgacaa tcaacggtac ggcaaaagag attccgctca
     3901 acagtgcctg tttcagctcc gttttgacac ttttcaccgg ttgcgtatct tgctgtacgg
     3961 tacgcgtctc atcgctacgc ttgagggtaa gcgcttgttg gatcaacgct tctgcatggc
     4021 gaatcggctc ggcaacaggc actgaaagcg cgggaatgcc attaaaacgc tcactctcct
     4081 tgatggcgac ttcagccgca aaaatacagg cggttgcact attgagttga tccgccgtta
     4141 aacgcccttc aatgccgtta gcgccttgtt tttcaacgta aacatttacg ccgagtttgc
     4201 gtccggcttt ttccagatat tccgcagcca tataggtgtg agcaataccc gccggacagg
     4261 ccgttacgca gacgatagtt ggcgcgttgg aaaaagaggc agaaggttgc gtgcctccct
     4321 tgtcatccag cgccgagagc aactcatcag gcgtcgtcgc cgactgtata cgcgcccgaa
     4381 tctcatcatc cgcaaggcgc gtggtcagcg ctgtcagcag ttgcatatgc gtagtacccg
     4441 cttcattggg gggaatcgcc agcagcacca ctaaatcaac tgcttccggg ccatcaacgc
     4501 cttcccactg aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct tttaccgcag
     4561 cagttttgcc atgcggcaca gccaaccctt cacctaaggc ggtcgggcca aggctttcac
     4621 ggcgatacac ttcttccaga aattgctcag tactggaaat tttccccaga gcagcaagac
     4681 gttgagtcaa cacgtggatg gcctcttcac ggctggtaaa gcgcgcattc agacacaacg
     4741 catcgcggtg ggtcagagtc gtcagattca tcatgatcct cacttgatcg tttttatagt
     4801 cgcgccagcg tgcccgataa aacaatacca ttatgtgatc aatttcactt gattgaaatt
     4861 atttgtattg tatttgtatt tgtattattt acaggatgaa tttatcgggc ataatggcta
     4921 aattcgtatt aatgagatac taaaaatggg acacaaaccc ttataccggc agattgccga
     4981 tcgcattcgt gagcaaattg cgcgtgggga gcttaaacct ggcgatgcgt tacctaccga
     5041 atctgcatta caaacagagt ttggcgtcag ccgggttacg gtgcgtcagg ctttgcgcca
     5101 gctagtcgag cagcagatcc tcgaaagcat tcagggcagc gggacttacg tcaaagaaga
     5161 gcgggtcaat tacgatattt ttcagttaac cagttttgat gaaaaactgt cggaccgtca
     5221 cgtcgataca cacagtgaag tcctgatatt tgaagtgatt ccggctgacg attttcttca
     5281 gcaacagcta caaatcaccg cgcaggatcg tgtgtggcat gtgaagcgcg tgcgctatcg
     5341 caagcaaaag ccaatggcgc tggaagaaac ctggatgccg cttgcgttgt tcccggatct
     5401 cacctggcag gtcatggaaa attcgaaata tcactttatt gaagaagtga agaaaatggt
     5461 tatcgagcgt agcgaacagg aaatcattcc tctgatgcca accgaagaga tgagccgtct
     5521 gcttaatatc agccagacaa agccgattct tgaaaaagta tcgcgtggat atttagttga
     5581 tggtcgggtg tttgaatata gccgcaacgc ttttaatacc gatgactata aatttaccct
     5641 gatagcgcag cgaaaatcat cgcgataagc acaaaaaagg ccatcatacg atggccaacc
     5701 atgtcgaaac cgacagccct tcgccattcc tgtagaggga agcggcgagg gctatttctt
     5761 attacagata tttatttcag aacagttttc agtgcttcac cgatatccgc caggctgcga
     5821 acggttttca cgcctgcggc ttccagagca gcgaatttct catccgcagt ccctttccca
     5881 ccggcaatga tggcacccgc gtggcccata cgtttgcctt tcggcgcagt cacaccagca
     5941 atgtagccca caactggctt ggtaacgtgc tctttgatgt acgctgccgc ttcttcttca
     6001 gcgctaccgc cgatctcacc aatcatcacg atcgcttcgg tctgcggatc tttttcgaac
     6061 atttcgagaa tgtcgataaa gttagagcca gggatcgggt caccgccgat accgacacag
     6121 gtcgactgac cgaaaccgta atccgtggtc tgtttaaccg cttcataggt cagtgtaccg
     6181 gaacgggaaa cgatacccac tttacccggt ttgtgaatgt gacccggctg gataccgatt
     6241 ttgcattcac ccggggtgat aacgcctggg cagttcgggc cgatcatacg aacgcctgct
     6301 tcatccagct tcactttcac ggtcagcata tccagcgtcg ggatgccttc agtgatggtg
     6361 ataatcagtt tgatgcctgc gtcgatggct tccagaatgg agtctttgca aaacggtgct
     6421 ggtacgtaga taacagaagc ggtagcgcca gtggcagcaa cggcttcacg cacggtgttg
     6481 aacaccggca ggccgaggtg ggtggtgccg cctttacctg gggttacgcc gccaaccatt
     6541 ttagtgccgt atgcaatggc ctgttctgag tggaaagtcc cctggctacc ggtaaagccc
     6601 tggcagataa ccttggtgtt tttatcgatt aaaatggaca ttatttcccc tccactgcgg
     6661 caacaacctg ctgagctgca tccgtcagac cttttgctgc aataatattc aggccgctgt
     6721 cagccagttt cttcgcgccg agttcggcgt tgttaccttc cagacgtacc acgaccggta
     6781 cgttaacacc cacttccgct accgcgccga tgataccgtc agcgatcagg tcgcaacgaa
     6841 cgataccgcc gaagatgtta accagaacgg ctttcacttt gtcgtcagag aggatgattt
     6901 tgaacgcttc ggttacacgt tctttggttg cgccgccgcc aacgtcaagg aagttagccg
     6961 gttcgccgcc gtgcagttta acgatgtcca tcgtacccat cgccagacct gcgccgttaa
     7021 ccatacaacc gatgttaccg tccagcgcaa cgtagttcag ttcccactgt gcagcctgtg
     7081 cttcacgctg atcttcctgc gactggtcac gcatttcgcg cagatcaggc tggcggaaca
     7141 gtgcgttgcc gtcagcgccc agtttgccgt cgaggcaaat cagatcgccc tgtttggtga
     7201 tgaccagcgg gttgatttcg atcaacgcca ggtcgcgctc caggaaaatg gtcgccaggc
     7261 ccatgaagat tttggtgaac tgctgaacca gtttaccttc cagacccagt ttgaacgcca
     7321 gctcgcgtcc ctgatacggc atcgggccag tcagcggatc aagcgcaact ttgtggatca
     7381 ggtgcggagt ttcttccgcc actttttcga tttccacgcc gccttcggtg gaggccataa
     7441 agaccacacg acgggaacta cggtctacaa cggcaccgag atacagctct ttagcgatat
     7501 cggtcgccgc ttcaaccaga atctggttaa ccggttggcc attggcatct gtttgatacg
     7561 ttaccagacg cttgccgagc cagttttctg caaaagcacg gatgtcttct ttgctgttta
     7621 caactttcac accgcccgct ttaccgcggc caccagcgtg aacctgacat ttcactaccc
     7681 acggaccggc accgattttt gaagcggctt cttctgcttc gcgcggagta gtacaggcat
     7741 aacccaccgg tgctggtaag ccatagcggg caaaaagttg ttttgcctga tattcatgta
     7801 agttcatgtg ttctgtccat ccttcagtaa tcgttatctt ttaaaccgta gaccgaatat
     7861 ttcgtatcgc ttccgggcat tgtcggtcac ccgtaggcct gataagacac gttaaacgtc
     7921 acatcaggca tcgcttgtcg gatgcggcgc ttgcgcctta tccgacctac attggtggtc
     7981 cccgtaggcc tgataagaca cgttaagcgt cacatcaggc atcgcttgtc ggatgcggcg
     8041 atagcgcctt atccgaccta cattggtggt cccgtaggcc tgataagacg cgtcagcgtc
     8101 gcatcaggct tcaattgccg gagaaggcga taatgcctta tccggtctac agtgcaggtg
     8161 aaacttaaac tactacacgt ccagcagcag acgcgtcgga tcttccagca actctttgat
     8221 cgttaccagg aagcccacgg attcgcgacc atcgatcaga cggtgatcgt aggacagcgc
     8281 caggtacatc atcggcagga tctcaacctg accattcacc gccatcggac gatctttgat
     8341 agcgtgcata cccagaattg cgctctgcgg cgggttgatg atcggcgtag acatcaggga
     8401 accgaacaca ccaccgttgg tgatggtgaa gttaccaccg gtcagatctt caacggtcag
     8461 cttgccgtca cggcctttga ctgccagctc tttgattttc ttctcgatgt ctgccatgcc
     8521 gagggtatcg acatcacgca gtaccggtgt caccaggccg cgcggcgtag aaaccgccat
     8581 gctgacgtcg aaatagttgt ggtaaaccac gtcatcgccg tcgatagacg cgttcacttc
     8641 cgggtaacgt ttcagggctt caaccaccgc tttcacgtag aaggacataa agcccagacg
     8701 gatgccgtgg cgtttttcaa acgcttcacc gtactgctta cgcagatcca taatcggctt
     8761 catgttgact tcgttgaacg tggtcagcat ggcggtggag tttttcgctt ccagcagacg
     8821 ctctgccaca cgcttacgca ggcgagtcat cgggacacgt ttttcactac gtgcagccag
     8881 agccggttgc gccgccggag cagccgctgc cggagcagac tctttcgccg gggctttcgc
     8941 cagatgtttt tccacatctt cacgagtcag acgaccaccc acaccggtgc ctttaatggc
     9001 gctggcgtcg agattgtgtt cagccagcag gcgacggatc gccgggctta acgcatcgtt
     9061 gttttgctct tccagagacg cctgctggcg ttgcgccgga gtggatgctt tctcttcaga
     9121 tttggcgctg gtttctttac cggcgctgtt gccttcacgc aggcgaccaa ggatctgacg
     9181 agacgttacg gttgtacctt catcttccag aaccgcatcc agaatgccgt ctgctgatgc
     9241 cggtacttcc agtaccactt tgtcagtttc gatttctacc agcacttcat cacgtacgac
     9301 tgcgtcgccg ggttttttat gccaggttgc gacggtggca tcggctacgg attcaggcag
     9361 gtcagggacc agaatatcta cgctactcat tgtgtatcct ttatttattc gacgttcagc
     9421 gcgtcattaa ccagatcttg ttgctgtttc tggtgaacgg acatataccc taccgccgga
     9481 gaggcggagg ccgggcggcc tgcataacgc agagaagccc caaacggaat cacttcacgg
     9541 aaatgatgct ggctgcagta ccatgcgccc tggttgagcg gctcttcctg gcaccagaca
     9601 aaatccttga cgtgagcaaa ctgctgcaac acttcctgca tcgctttatg cgggaacggg
     9661 tagagttgct cgatacgcac aatggcgaca tcgtgttgat tgttcttacg acgctgttcc
     9721 agcaggtcgt aataaacctt accagaacac attactacgc gcttcacgcc cttcggatca
     9781 agctcgtcga tttcaccgat ggctggcagg aaggtgccgt tcgccagttc ttcgaggctg
     9841 gaaaccgcca gcggatgacg cagcagggat ttcggcgaca tcacgaccag cggacgacgc
     9901 atcccgcgca gcgcctgacg acgcagcatg tggtaaacct gtgccggggt agacggtacg
     9961 caaacctgca tgttttgctc agcacaaagt tgcagataac gttccagacg cgcggaggag
    10021 tgctccggcc cctgcccttc gtaaccgtgc ggcagcaaca tcaccaggcc acacatccga
    10081 ccccatttct gttcgccaga agagatgaac tggtcgataa ccacctgcgc accgttggcg
    10141 aagtcaccga actgcgcttc ccagatagtc agagtgcgtg gttctgcggt ggcataaccg
    10201 tattcaaacg ccagcactgc ttcttcagac agtacggagt cccagacgcg gaacgcgccc
    10261 tgcccgttat ggatgtgttg caacggcgtg taagtggaac cgttagactg gttgtggatc
    10321 accgcgtggc ggtggaagaa ggtaccgcga ccggagtctt cacccgacag gcgaaccgga
    10381 atgccttcgt caaccagcgt ggcgtaagcg aggttttccg caccgcccca gtcgaacagt
    10441 ttctcaccgg cagccatcgc ctggcgatcg ccataaatct tggcaacgcg agactgcatt
    10501 tcaactgctt ccggcaccgt gctgatgcgt ttcgccagct cctgcaggcg cttcatctca
    10561 actttgttcg ggtactcttc gtcccattcg tggttgaggt acggcgacca ggtgaaagag
    10621 tgcatgttca tcggacgcca ctctgccact acgcaatcgc cagcatccag cgcatcgcgg
    10681 tacaggttaa ccatctcggt ggcatcttcc agcgtcgcca ctttttcctg ctccagcttg
    10741 tcagcgtaga ttttgcgcgg tgtcggatgt tttttgattt tctgatacat cagcggctgg
    10801 gttgcgctcg gctcgtcggc ttcgttgtgg ccgtgacggc ggtagcacac caggtcgatg
    10861 aagacatcac gtttaaaggt gttacggaaa tcgagcgcca gacgggtcac aaaggcaacg
    10921 gcttccggat catccgcgtt aacgtggaaa atcggtgcct gaaccatctt accgatatca
    10981 gtacagtacg gtgtagaacg agcatccagc ggattagagg tggtgaaacc aacctggttg
    11041 ttgataacga tacgtaccgt accgccaact tcataaccac gcgctttcga catgttcaga
    11101 gtttcctgaa ccacgccctg cccggtcact gcggcgtcac cgtggatggt gattggcagc
    11161 actttgttgc tgctcggctc atcaagtctg tccagacggg cacgaacaga accgataact
    11221 accgggctta caatctcaag gtgagacggg ttaaacgcca gcgccaggtg caccaggccg
    11281 ccatcggtct ggaagtcaga cgagaagccc atgtggtatt tcacgtcacc cgtgccgagg
    11341 tgttctttat gtttaccggc aaactcgtcg aacaagtctt gcggtttttt acccagcacg
    11401 ttcaccagca cgttcagacg accacggtgc gccatcccga gaaccacttc ccgggtgccg
    11461 ctgttgccag cgtggcggat catctcttta agcatcggga ttaacgcgtc accgccttcc
    11521 agcgagaagc gttttgcacc agggaatttt gcaccgaggt aacgttcaag gccttcagcg
    11581 gcggtcagtt cgcttaagaa gcgttttttc tcttcgctat tgaaagtcgc gcgaccagac
    11641 tcgatacgct gttggatcca gcgtttttct tcggtgctgg tgatgtgcat atactcggca
    11701 ccaatcgggc cgcagtaggt ttgcttgagg gcttccagca gctcgccgag tttcatggtt
    11761 tctttgccgc tggcaaatga accgacgttg aaggtctcct ggaagtctgc ttcggtcaga
    11821 tcgtggaaag acggatccag atcggccact ttatcttgct gccacagtcc cagcggatcg
    11881 agattcgcat gctggtgacc acggaagcgg tatgcgttaa tgagctgcag gacttttacc
    11941 tgcttcacat tggtgtcagg gtcggagatc gttgaagagt aacgtgaagc gtctttcgcc
    12001 aggcggcgga aatattcacg cgtttgagag tggaattgat ccggtttgac tcccgtacca
    12061 ggtaactgct ggaacgtcga acgccagtta gcgtcaaccg agtcaggatc ggttaagaag
    12121 tcttcataga gctgttctat ccagctctgg tttgcgccag agaggtaaga agagtccaac
    12181 caggctttca aagcgctgtt ctgcatcgtg atcccttaag catctttttt atgcttactt
    12241 cgccgtggat actaccacgc acagtgatgt gcgtgccggg gttcacttgc gagctcttgt
    12301 ctacgtagtg gctcgcgaag gaacctttaa aaactgtctt agtgtcaggg gcagttttta
    12361 aagatttcct gcatttcttt gttacgcctg atgcgctgcg cttatcaggc ctacgggtaa
    12421 tgacttgtag gccggataag gcgtttacgc cgcatccggc actaggttgc ctgatgcgac
    12481 gcttgcgcgt cttatcaggc ctacggttta cgcattacgc tgcaacaaca tcgacttgat
    12541 atggccgatg gcgcgcgtcg ggttcagccc cttcggacat acactgacgc agttcatgat
    12601 gctgtgacag cggaatacgc tgaatgcatc gctcaaaccg tcgaggcggc tgtcagtctc
    12661 ggtatcacgg ctatcgatca ggaaacgata tgccgctaac aagcctgccg ggccgataaa
    12721 cttatcggga ttccaccaga aagacggaca agaggttgaa caacatgcgc agagaataca
    12781 ttcatacagc ccgtcgagtt tttcgcgctg ctctggcatc tgtaaatgct cgcgagctgg
    12841 cggattttgt ccattattca acaggtaagg cttaattttc tcatattgcg catagaattg
    12901 tcccatgtct accaccaaat cgcggatcac cggtaaacct ggcagcgggc gaatcacaat
    12961 cttcttgccc ggctggttga gtgccgaaat cggggtaata caggccagac cattcttgcc
    13021 gttcatgttc agaccgtcgg aaccgcacac accttcacga caggagcggc ggaacgacag
    13081 gctgggatct ttctctttca gctgaataag cgcatccagc agcatcatgt cgcgaccttc
    13141 ttccgcttcc agggtgtaat cctgcatacg cggagcatca tcaacatccg ggttatagcg
    13201 ataaattgaa aactcgagtc tcattttcct gtctccgcat tagtaagtac gaatcttcgg
    13261 cgggaatgcc gggcgcagtt tcggttccat gttgacgctt cggcgcgtca tggattccga
    13321 ctctggcaga tacagggagt ggcacagcca gttttcatca tcacgatccg ggaagtcgaa
    13381 gcggctatgc gcgccacggc tttcggtacg gaagttggca gaaacagccg ttgcatacgc
    13441 cgtttccatc aggttatcca gttccagaca ctcaacgcgc tgggtgttga actcgctgga
    13501 agtgtcatcc agacgggcat ttttcagacg ctcgcggata actttcaact gctcaagccc
    13561 tttcgccatc gcatcacctt cacggaagac cgagaagtta tgctgcatac attcttgcag
    13621 cgctttacga atcgccaccg gatcttcacc gttacgatta ttgttccagc ggttcagacg
    13681 atccagagat gcttcaacat cagactcgct ggcatcgcgc agtgcgccct gctcggcgat
    13741 agactcttgc agatgcagac ccgctgcgcg accaaagacc accaggtcca gcagcgagtt
    13801 accgcccaga cggttagcac cgtgtaccga tacgcaggca atttcaccta ccgcgaacag
    13861 ccccggaaca accacatctt cgcctttctc attcacagtc agcgcctgac cggtcacttt
    13921 ggtcggaata ccgcccatca tgtagtgaca ggttgggata accggaatcg gctctttcac
    13981 cggatcaacg tgagcgaagg tacgggaaag ctccaggata cccggcagac gggattcgag
    14041 aacttcttta cccaggtgat cgagtttcag tttcgcgtgt ggcccccacg gaccatcaca
    14101 gccgcgacct tcacggattt caatcatgat ggaacgcgcc accacatcac ggcccgccag
    14161 gtctttggcg ttcggcgcat aacgctccat aaagcgttcg ccatgtttgt tcagcagata
    14221 accgccttca ccacggcaac cttcggtgac cagtacgccc gcaccggcaa tgccggtcgg
    14281 gtggaattgc cacatttcca tatcctgcac cggtacgcct gcgcggatag ccatgccgac
    14341 accgtcgcca gtgttaatgt gggcgttagt ggtggactga taaatacgcc ctgccccgcc
    14401 agtcgccagc acggtagcgc gggctttgaa ataaaccact tcaccggttt cgatgcacag
    14461 tgcggtacaa cccaccaccg cgccatcctg gtttttcacc agatccagcg cataccactc
    14521 ggagaaaatg gtggtgtggt ttttcaggtt ctgctgataa agcgtgtgca acagtgcgtg
    14581 accggtacgg tcagccgctg ccgcagtgcg tgccgcctgc tcgccgccga agtttttcga
    14641 ctgaccgcca aacggacgtt gatagatacg accatcatca agacgcgaga acggcaggcc
    14701 catgtgttcg agttccagaa tcgcttccgg cccggtttta cacatatatt caatcgcgtc
    14761 ctggtcaccg atatagtccg acccttttac ggtgtcgtac atatgccatt cccagttatc
    14821 ttcatgggta ttacccagcg caacggtaat gccgccttgc gcagaaacgg tatgggaacg
    14881 ggtcgggaag actttagaga gcagcgcaca ggtctggccg ctctgggaaa tttgcagcgc
    14941 cgcgcgcata cctgcgccgc cggcaccaat cacaactgca tcaaattctc tgactggcaa
    15001 tttcatcaca caccccacac cacaacgaat ccataaatca cgtaaaccac cagtgcaacg
    15061 acaatcacca gttgcagcat caggcgcaaa gccagcggtt taacgtagtc ggtcaacacc
    15121 tgccacatgc cgatccaggc atggatcaag atagaaaaca gcgccagcag ggtgaacact
    15181 ttggtgaacg cagaggcgaa gaaaccgatc cagacttcat atgtcagctc gccactggta
    15241 gcgaaaaaac cgaccatata aatgatgtag agcgtcagga cgatagcggt agcgcgaacg
    15301 aggatgaaat catgtacgcc attgcgtcct aatgcggagg cgttgcttac catacgagga
    15361 ctcctgcgag aagtgaaagc acgacagtaa taacaaagga gattttggcg gagcgtttac
    15421 ccgcttcgaa tgtttcttcc agatagccaa aatccatcat catgtggcga atacctacga
    15481 cgacgtgata cgccagagcg gtaaggatgc cccacatgat aaatttgacg aagaagctgc
    15541 ccataatcgc ggaagcttgc tcgaaacctt caggggaaga gaggctggta cccagaagcc
    15601 acagcaggat gcccactgca acaaaggtga tcacaccgga aacgcgatgg agaatggacg
    15661 ctatcgccgt gatggggaac cggatggtct gtaggtccag attaacaggt ctttgttttt
    15721 tcacatttct tatcatgaat aacgcccaca tgctgttctt attattccct ggggactacg
    15781 ggcacagagg ttaactttct gttacctgga gacgtcggga tttccttcct ccggtctgct
    15841 tgcgggtcag acagcgtcct ttctataact gcgcgtcatg caaaacactg cttccagatg
    15901 cgaaaacgac acgttacaac gctgggtggc tcgggattgc agggtgttcc ggagacctgg
    15961 cggcagtata ggccgttcac aaaatcatta caattaacct acatatagtt tgtcgggttt
    16021 tatcctgaac agtgatctag gtcacgataa caacatttat ttaattttta atcatctaat
    16081 ttgacaatca ttcaacaaag ttgttacaaa cattaccagg aaaagcatat aatgcgtaaa
    16141 agttatgaag tcggtatttc acctaagatt aacttatgta acagtgtgga agtattgacc
    16201 aattcattcg ggacagttat tagtggtaga caagtttaat aattcggatt gctaagtact
    16261 tgattcgcca tttattcgtc atcaatggat cctttacctg caagcgccca gagctctgta
    16321 cccaggtttt cccctctttc acagagcggc gagccaaata acaaacgggt aaagccaggt
    16381 tgatgtgcga aggcaaattt aagttccggc agtcttacgc aataaggcgc taaggagacc
    16441 ttaaatggct gatacaaaag caaaactcac cctcaacggg gacacagctg ttgaactgga
    16501 tgtgctgaaa ggcacgctgg gtcaagatgt tattgatatc cgtactctcg gttcaaaagg
    16561 tgtgttcacc tttgaccctg gcttcacttc aaccgcatcc tgcgaatcta aaattacttt
    16621 tattgatggt gatgaaggta ttttgctgca ccgcggtttc ccgatcgatc agctggcgac
    16681 cgattctaac tacctggaag tttgttacat cctgctgaat ggtgaaaaac cgactcagga
    16741 acagtatgac gaatttaaaa ctacggtgac ccgtcatacc atgatccacg agcagattac
    16801 ccgtctgttc cacgctttcc gtcgcgactc acatccaatg gcagtcatgt gtggtattac
    16861 cggcgcgctg gcggcgttct atcacgactc gctggatgtt aacaatcctc gtcatcgtga
    16921 aattgccgcg ttccgcctgc tgtcgaaaat gccgaccatg gcagcgatgt gttacaagta
    16981 ttccattggt cagccatttg tttacccgcg caacgatctc tcctacgccg gtaacttcct
    17041 gaatatgatg ttctctacgc cgtgcgaacc gtatgaagtt aatccgattc tggaacgtgc
    17101 tatggaccgt attttgatcc tgcacgctga ccatgaacag aacgcctcca cctccaccgt
    17161 gcgtaccgct ggctcttcgg gtgcgaaccc gtttgcctgt atcgcagcag gtattgcttc
    17221 actgtgggga cctgcgcacg gtggtgctaa cgaagcagcg ctgaaaatgc tggaagaaat
    17281 cagctccgtt aaacacattc cggaatttgt tcgtcgtgcg aaagataaaa atgattcttt
    17341 ccgcctgatg ggcttcggtc accgcgtgta caaaaattac gacccgcgcg ccaccgtaat
    17401 gcgtgaaacc tgccatgaag tgctgaaaga gctgggcacg aaggatgacc tgctggaagt
    17461 ggctatggag ctggaaaaca tcgcgctgaa cgacccgtac tttatcgaga agaaactgta
    17521 cccgaacgtc gatttctact ctggtatcat cctgaaagcg atgggtattc cgtcttccat
    17581 gttcaccgtc attttcgcaa tggcacgtac cgttggctgg atcgcccact ggagcgaaat
    17641 gcacagtgac ggtatgaaga ttgcccgtcc gcgtcagctg tatacaggat atgaaaaacg
    17701 cgactttaaa agcgatatca agcgttaatg attgattgct aagccgttta ctttccggac
    17761 ccgcctttaa tagagcgggt tctttttata tggctgaaca taataaattg taaaaattaa
    17821 accagataca aatacccatt aataaggtga gaaaatgtga caagtcacat actgagcaga
    17881 atgataagtt tatttttata ctttcctgct ggtagcattg ttaacccaat gaaaattatg
    17941 catatccatc ccaccccatc cttcatctca ttaaacaata aatttatgca ctcaattatt
    18001 ccattcttta tataacaaga tttttaatat tgttaaataa aaccaaatac attcaactgc
    18061 cagttaatat attcttgcca cgcaacattc tatctcatta tttaaaggac tccgttatgt
    18121 ttaaaggaca aaaaacattg gccgcactgg ccgtatctct gctgttcaca gcacctgttt
    18181 atgcggctga tgaaggatcc ggtgaaattc actttaaagg tgaagttatt gaagcaccgt
    18241 gtgaaataca tcaggatgat attgataaag aggttgaact cggtcaggtg accaccagcc
    18301 acattaatca gtctcatcac agcgatgccg ttgctgtcga cctgcgctta gtcaactgtg
    18361 atctggaaaa ctccagcaac ggttccggtg gcaagatttc taaagttgca gtcacctttg
    18421 atagctcagc gaaaaccacc ggcgcagatc cgattctcaa caacaccagc acgggtgaag
    18481 ccaccggcgt cggcgtacgt ttaatgaata aagatcaaag caatatcgtt ttaggcaccg
    18541 ctactccaga tatcgacctg gctccgacct ccagcgaaca aacgctgaat ttctttgcct
    18601 ggatggaaca aattgatcag gctacacctg taacgccagg cgcagttacg gcgaatgcaa
    18661 cctacgtgct ggattataaa taagtattac tttggcggcg ctatcgccgc ctttcataaa
    18721 atatttatca ggtatggaca ccgtgaatat ttatcgactc tcttttgtat cctgcctggt
    18781 cgtggcaatg ccttgcgcat tggcggtcga attcaacctt aatgttctcg ataaatcgat
    18841 gcgcgaccgc attgatattt cattattaaa ggaaaaagga gtcattgctc ccggtgagta
    18901 ttttgttagc gttgcggtaa ataataacca aatcagtaac gggcaaaaga ttaactggca
    18961 caaaaatgac gataaaacca ttccgtgcat caatgattta ctggtcgata aatttggctt
    19021 aaaacctgaa gtccgtcagt cgttaccatt gataaatcag tgcgtcgatt ttagctcccg
    19081 acctgaaatg ctcttcaatt tcgatcaagc caatcagcaa ctaaatatca ccattccgca
    19141 agcctggctg gcgtggcact cagaaaactg gaccccaccc tccacatgga aagaaggtgt
    19201 cgccggtatc ctgatggatt acaacttgtt tgccagcagc taccgcccac aggacggcag
    19261 cagcagcact aacctgaacg cctacggtac caccggaatt aacgccgggg catggcgctt
    19321 acgtagtgat tatcagttga atcagactga tagcgatgat aaccatgaac agtcaggcgg
    19381 aatatcgcgc acctatcttt ttcgtccatt accgcaatta ggctctaaat taaccctcgg
    19441 cgaaacggat tttagttcca atattttcga cggtttttct tataccggcg cggcactggc
    19501 aagtgacgag cgaatgttgc catgggaact acgcggctac gccccacaaa ttagcggtat
    19561 tgcacagacc aatgccacgg tgacgatcag tcaatcaggc cgcgtcattt accagaaaaa
    19621 agtcccacca ggcccattta tcattgacga ccttaatcag tctgttcagg gcacactgga
    19681 tgtcaaagtg acggaagaag atggtcgggt gaacaatttc caggtttcgg cagcatcgac
    19741 gcccttcctg actcgtcagg gacaggttcg ctataaactg gccgcgggtc agccacggcc
    19801 ctccatgtca catcaaactg aaaatgaaac cttttttagc aatgaagttt cctgggggat
    19861 gctgtcaaac acctcgctgt acggcggcct gctgctttct ggtgatgact accattctgc
    19921 cgcaatgggt attgggcaaa atatgctgtg gcttggtgcg ctgtcgtttg atgtcacgtg
    19981 ggccagtagc cattttgata ctcagcagga cgagcggggc ttaagctacc gttttaatta
    20041 cagcaaacaa gtggatgcca ctaacagcac gatttccctc gccgcttatc gtttctccga
    20101 tcgtcatttt cacagctacg ccaactatct ggatcacaaa tacaacgaca gcgatgcgca
    20161 ggacgaaaaa cagacgatca gcttatctgt gggccaaccg attaccccac taaacctcaa
    20221 tctttacgcc aacctgctac atcaaacctg gtggaatgca gacgcctcca cgaccgccaa
    20281 catcacagcc ggttttaatg ttgatattgg tgactggaga gatatctcta tttcgacgtc
    20341 attcaataca acccattacg aagataaaga tcgcgacaac cagatttacc tgtcgatttc
    20401 gctccccttc ggtaatggtg gtcgggttgg ttatgacatg caaaacagta gccacagcac
    20461 cacacaccgc atgtcgtgga acgatacgct ggatgaacgt aatagctggg gcatgtctgc
    20521 cggactgcaa tccgaccgtc ctgacaatgg agcccaggtg agcggtaact atcagcacct
    20581 gagttcagcg ggtgagtggg atatttctgg tacctatgcc gccaatgatt acagttccgt
    20641 cagcagcagc tggagcggtt ctttcaccgc aacccaatat ggtgcagcgt ttcatcgccg
    20701 cagctccacc aatgaacctc gcctgatggt cagcaccgat ggcgtggcag atattccggt
    20761 tcagggcaat ctcgactaca ccaaccattt tggcattgcg gtggtgccgt tgatttccag
    20821 ttatcagcct tccaccgtgg cggtgaacat gaatgactta cccgacggcg taacagttac
    20881 agaaaacgtt atcaaagaaa cgtggattga aggcgcgata ggttacaaat cactggcttc
    20941 ccgttccggt aaagacgtta acgtcatcat tcgcaacgcc agcggtcagt ttcctcccct
    21001 cggagcggat atccgccagg atgacagcgg cattagcgtg gggatggttg gcgaggaagg
    21061 acatgcctgg ttaagcggag tcgctgaaaa tcaaaagttt accgtggtct ggggtgatag
    21121 ccagcattgc tcgctccatc ttcctgaaca tatggaagac accgcaaatc gcctgatttt
    21181 accttgtcat taattaagga aatgaaaatg acatttatga aaggactacc ccttttgttg
    21241 ttggttgcca gtctgtgcag ccacgctgca ctacaacccg atcgcactcg tattgtgttt
    21301 aacgctaatg ataaagctac cagtctgcgg gtggataatc gtagcgataa actcccctac
    21361 ctcgcttatt cctggctcga aaatgaaaaa ggggagaaaa gcgatgatct tctggtcgcc
    21421 ctgccgccga tacaacgtct ggaaccgaaa gcaacaacgc aggtacggat agtaaagcaa
    21481 gcctcgacca caaaattacc gggcgatcgc gaaacgctat ttttctacaa tatgcgtgaa
    21541 attcctcccg ccccggaaaa aaacagcgac catgccgttc tgcaagtcgc cattcagagt
    21601 cgtatcaagg tgttctggcg accggctgca ttacgcaaga aagcaggaga aaaggtcgaa
    21661 ctgcagttac aggtcagcca gcagggcaac caactgacac tgaaaaatcc taccgcgtat
    21721 tatctgacca ttgcctacct gggacgaaat gaaaaaggcg ttctccccgg cttcaaaacc
    21781 gtgatggttg cgccttttag tacggtcaac acaaacaccg gaagctatag cggcagccag
    21841 ttctatctcg gttatatgga tgattacggc gcgttacgca tgacgatgct caactgcagc
    21901 ggacaatgcc atttacaagc cgtggaggcg aagaaatgag tgctggcaag ggagtgttgc
    21961 tcgtcatctg tctgttattt ctgccgttga agtctgcgct tgccctgaac tgctactttg
    22021 gttcatccgg tggctcggta gaaaaatcag aagcgattca accttttgct gtaccaggca
    22081 atgccaaact cggcgataag atttgggaat ctgacgatat taaaattccc gtctattgtg
    22141 acaacaatac caacggcaat tttgaaagtg agcatgttta tgcctgggta aacccctacc
    22201 cgggggtgca ggatcgctat tatcaactcg gcgtaactta caatggcgtt gattatgacg
    22261 ccaaccaggg aaaaagccgc atcgacacta accagtgtat tgacagtaag aacattgaca
    22321 tttatacccc tgagcagatt atcgcgatgg gatggcagaa caaaatttgc tcgggtgatc
    22381 ccgctaatat tcacatgtcg cgtacctttc tcgctcgcat gcggttatac gtcaaaatac
    22441 gagaaatgcc gccgcatgat tatcaaagta cgcttagcga ctatatcgtc gtgcaatttg
    22501 acggtgccgg tagcgttaac gaagacccta ctgcccaaaa cctgaaatat catattactg
    22561 gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt ttccatttca ccggaaacac
    22621 aggtgattga ttttggtaaa tttaatttgc tggatattcg tcgccacaca atgtcgaaaa
    22681 cattcagtat taagacgaca aaatcacaaa atgaccaatg caccgacgga tttaaagtca
    22741 gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga taaagcattg ttgattggta
    22801 atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc ttataccttc aataaatatg
    22861 ctgaatacgc cgatttcacc agcgacatgt tggtctacga aaaaacctat acagctgaac
    22921 tttcgtccat tgcaggtacc cccatcgagg ctgggccctt cgatacagtg gtgcttttta
    22981 aaattaacta taactgattt gccatgatgc cctttgcagc cggaaagggc atctctttca
    23041 gaaaattgct gattttcccc ttttccttga ttgctgttat tttttcctgg cccggctttc
    23101 tttatgctgt atcgatacac caaagagaaa ataatgaggg agcgaaggat gccagttttg
    23161 cagtggggaa tgttatgtgt gctatcactt ctcctttcta ttggtttcct cgcggtacat
    23221 ctcccggcgg cgctattact tgggccgatg attgccggga tcatctttag tatgcgcgga
    23281 attactctgc aacttccccg ctctgctttt ctcgccgccc aggccattct tggctgcatg
    23341 attgcgcaaa acctcactgg ttcgattctc accaccctgg ctgtcaactg gccgatcgtg
    23401 cttgcaattt tactggtaac gctgctttcc agcgccattg tgggttggtt attggtgcgc
    23461 tatagctcat tgcctggaaa taccggtgcc tggggctcct ctcctggcgg cgcggcggca
    23521 atggtcgcta tggcgcaaga ttacggcgca gatattcgcc tggtggcgtt tatgcaatat
    23581 ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca cccgcatgat gctgggcgat
    23641 aacgctgaag cggttaacca gcatatcgtc tggttcccgc cagtgagcat taatctcctg
    23701 cttactattt tgctcgcggt cgttgccggt acagtgggat gcctgctgcg tcttccttcc
    23761 ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc agtctggtca gctcatcacc
    23821 atcgaactac cggaatggct gctggcgatg gcgtatatgg caattggctg gcggattggt
    23881 cttggtttcg ataaacaaat cttactgcgg gcactacgcc cgctaccgca aatcctgctg
    23941 tcgatttttg ctctgctggc tatttgtgca ggtatggcgt ggggactgac ccggtttatg
    24001 cacattgatt ttatgactgc ctacctcgcc accagccccg gcgggcttga taccgtggcg
    24061 gtcatcgccg cagggagcaa tgccgatatg gcgctcatta tggcgatgca aaccctgcgc
    24121 ctgttcagta ttttgctgac agggcctgcc attgcacggt ttatttcaac ctatgcgccg
    24181 aaacgtacgg cctagtgctg acagccaggg caccagtaaa acgggcgaga tgacagcgtg
    24241 gttttctcaa tgatgccgcc acagcgttcg cacggttcgc catcacgatg aaaaacctta
    24301 aagcgaaaca gcgccccatg atgcttattc tcatccacct gcccccgcgt agcgtaagaa
    24361 aaccgaggaa tatccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc attgaggtct
    24421 ttcgctttat gatttccagt caacccaacc tgccagagga tctccacccg caaataattg
    24481 ccaagtcctg ccagaaacgc ctgatcgagc agtaatccag caaactgacg gttacgaaag
    24541 cgcggcgaca ataatcgttc ctttaccacc tccggcgtca gattcggatc cagcacatcg
    24601 gggccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt ccggggtcaa catctcaata
    24661 tccgaggcgc tataaagcag aatggttttg tcaaccgttt gcagttttac ccgcaatact
    24721 cgcgtggtct gcggctcttc gccggtatca accacgcgcc agatgccgta aagctgatta
    24781 tggctgtaga gcgttaagtc gttggaaaaa tgagttaaca acgccttacc acgcgtttcc
    24841 acatgggtaa cgtgttgacc gataagttgt gattgataag tttttaactg cgggaaggca
    24901 aaccagacat cagttagtgg tttgcctttg atcgccgcct ccaggttatc cgctgcacgg
    24961 cggatctccg ggccttcagg catggtgata tccttttttc attctttatt aagcggggtt
    25021 atgctgcgac aacaatcccc tttttggcaa atgtagctcg taatcggcgg gcgaacgcca
    25081 gagcgtgctc gccgtcgcca tgcaggcaga ccgtttgcgc cgcgaccgtt gcccattcgc
    25141 cggtgatact tttgactctg ccgtgttgca ccatctccag cgtttgcgcc aacgcctgtt
    25201 cttcgttttc aatcaacgcg cctggctgac ttcgcggcac cagcgagccg tcagcctgat
    25261 aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa
    25321 tcagctcgct tcctgccagc ccaacgagaa ccaatgccgg atcgcaagcg tataccgctc
    25381 tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg gttgtacaac atgccgtgcg
    25441 gtttgacatg acgcattacg ccgccttgcg cacgggcaat cgttgccagc gcgccaattt
    25501 gatacagcgt ctgggcgtaa acggtttccg gcggcagctg catggcgctg cgaccaaaat
    25561 tttccctgtc gggaaaactc gggtgagcgc caatcgcgac accatttttt attgcttcac
    25621 gcacgcaagc ctgcatggtt tgcgcatcgc ctgcatgaaa tccacaagca atattggcag
    25681 aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca
    25741 ggtcaatttt cattgtgcag ccgccacgct aattgttgga aataacgttg ttgatcttgc
    25801 cgcgctttca gtgcctcttc cagtgaacac tggacaaaat gaatcggctg accgagcgga
    25861 atttgcgcca gatggtacat atcagcctca atgatacagg caatacgcgg gtaaccaccg
    25921 gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt tatgtggcac ctgcaccacg
    25981 cccggtaaca aaccgtgaga taacagttcg cgatcggtgg tgcgttttaa aatttgcccc
    26041 tgtaagcgat agcccattcg gttactttgc gagctaagct gccagggcga acgccagaat
    26101 gcatcctgcg aggcgcgatc aaactcatga tattccggcc ccggcaaggc gcgaatgcgg
    26161 ttgccccaca acagctgttt aacgccctgc gcttccataa aatcacgctt cgatttgcca
    26221 atcgggagtc ggtcaccatc cttcagtaaa cggccttcca gcccgccaat ccccacgttg
    26281 agatcggtgc tgcatgaccc cattaccggc ggaacatcaa taccacccgc gaccgccaga
    26341 taactgcgca tcccgtgctg cgggcgttta agcgttaaac gctggcctgc tttcatcggc
    26401 aatcgccagc cggtccagac ggcattatca tccagccgcg cttcgcaacc ggcacccgtc
    26461 agagcaaacc acccatcagt ttcgaactca acagttaact gaccgagcgt gatctccagc
    26521 gcgggggcat tggcgtcatt acccaccagt aggttagcaa tgcgtaacgc gggcatatcc
    26581 agtgcgccgc agtggctgat acccgactgg cgaaaaccgt gacgaccgcc atcctgcaca
    26641 gtggtataca tgcccgcacg aataatcttc agcatactcc ctccttctgc ggtacaaagc
    26701 gcacgctgtc tcccggacgt aataagatgg gttcgtcacg cgccggatca aacaggctga
    26761 gtgaggtatg accaatcaac tgccagccac ccggcgttgc cagcggataa acaccagtct
    26821 gcggcccgcc gatcccgaca gaacctgccg gaacgagtaa gcgcggttca gcgcgccgtg
    26881 gcgtgtgtag ttgttccggc aaactcccga gatacgggaa gcccggttga aaacctaaaa
    26941 accagaccac gtattccacg gaggagtgca attcaacaac ctgtttttcg ctcaacccgc
    27001 aatgcgccgc gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc accggaattt
    27061 caataaagcg agactcaggt tccagggcct cgctctcctc ccaccagcgt tgcaaacgct
    27121 ctatggcatc cagcgccagc gactcaggat tacgcaaaat caccgtaata ttgttcatgc
    27181 cgggaatggc ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt cgccagatcc
    27241 gtttctggct agccagcgtc accggcggtt ccagttccag cactaccgcc gtttcaccta
    27301 tcagataaca acgcgctcgt tgcactttcg atccctctga ttattcgtta tgcaggatta
    27361 ggaatatcaa taaaggtcac atcaagatcg gtattttcat tcagccactc gctcaatgcg
    27421 cgaataccac cacgttcagt ggcatggtga cctgcagcat aaaaatgcaa tccctgctcg
    27481 cgggctgaat gaatggtctg ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa
    27541 cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc accaggcgac gcgctggacc
    27601 acctcaggtc cggtatcgcc acaccataac ggcttacgtc ccagacgcgc ttcaatccag
    27661 gaggctagtt ccagtcccgg cacaggcatg gtcagctctc cccacggcac caacggctca
    27721 atttcgccca tgaccgtgat ccccagtaac gccgccagct gtgcgttatt gcccagctca
    27781 ggatgtgcgt caagcggcaa atgccagcca tacaggttga tatcattcgc cagcaacgtt
    27841 tttaaacggt tacgcttcat tccgcgaatg accggagact ctcctttcca gaagtagcca
    27901 tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa agcctggctg
    27961 gcggtgacac cggtaacaat tttttgcacc gtctctttgc cttccacctg caaaccattc
    28021 ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt taatcagttg ttccagttcg
    28081 gtgtttttca tcttcgcgcc ttccgtattc tgatagctac accgttaaca tagcgttacc
    28141 tcgccagttc gtcgatagcc aatcttttga catattaaga aacaaacccg acgccaaaag
    28201 acgcaaattg tttatatcat cacgcttcag ctaatgtata tttatgcaat cagacagcat
    28261 attgcataaa taatcagatc tgtatcaaag ttttaacttt aaggaaacac aagcacgccg
    28321 cgcaactatt acgtgacgtt gccgtcaatt gtggaattat tatgaataaa cacgcatcac
    28381 agccgcgcgc tatttactat gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg
    28441 gcatgcgtgc cctgctgatt ctctatctca ccaatcaact aaaatacaac gatactcacg
    28501 cctacgagtt atttagcgcc tactgttcgc tggtgtatgt cacgccaatc ctcggtggct
    28561 ttttggcgga taaagttctc ggcaatcgca tggcggtgat gctgggggcg ttgttgatgg
    28621 cgatcggtca tgtggtgctg ggtgccagtg agatccatcc gtcattcctc tatctgtccc
    28681 tggcgattat cgtctgcggc tatggcctgt ttaaatctaa cgtaagctgt ctgctcggcg
    28741 agctgtatga gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg tatgcggcgg
    28801 gtaacgtggg gtctattatc gcacctatcg cctgtggtta cgcccaggaa gagtacagct
    28861 gggcgatggg ctttggcctg gcggcggttg gcatgatcgc gggtctggtc attttcttat
    28921 gtggcaatcg tcatttcact catacccgcg gcgttaacaa aaaagtactg cgtgcgacaa
    28981 actttctcct gccgaactgg ggatggctgc tggttctgct ggtggcaacg cctgcactga
    29041 ttaccatact gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg actattattg
    29101 gcctgggtgt actggcaaaa atttatcgca aagcagaaaa ccagaaacag cggaaggagc
    29161 tggggctgat tgtgacgctc accttcttca gtatgttgtt ctgggccttc gcacaacagg
    29221 gcggtagctc gattagcctt tatatcgacc gcttcgttaa ccgcgatatg tttggttata
    29281 ccgttccgac cgcgatgttc cagtcgatta atgccttcgc agttatgctg tgcggtgtgt
    29341 tcctggcgtg ggtggtaaaa gagagtgtcg cgggtaatcg taccgtgcgc atctggggga
    29401 aatttgctct tggccttggc ctgatgagcg ccggattctg cattctgacc ttaagcgccc
    29461 gctggtccgc aatgtatggt cactcttctc tgccactgat ggtattaggc ctggcggtga
    29521 tgggctttgc ggaactgttt atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa
    29581 tccccggtgt gaccggcgta ttaaccggca tctacatgct gctttctggc gcgatcgcga
    29641 actatctggc gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat gcttccgggg
    29701 cgatcaacta ctccatcaat gcatatattg aagtatttga tcaaattacc tggggcgcac
    29761 tggcgtgtgt aggactggta ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc
    29821 gcgcgctggc gctggagtct taataggcca gcaccgatgc ctgatgcgac gcttgacgcg
    29881 tcttatcagg cctacgtatt tcctgcaatt tattgaattt gcacaaattt gtaggctgga
    29941 taaggcgttc acgccgcatc cggcatctgg gctcgattgc ctgacgcgtc tgttatttcc
    30001 ccttccgcgc cgcctcatac gctgccaacg tttgtactct cgcttctttg tgctcgacta
    30061 tcggttgcgg ataatccagc gtcacacctg ctttctgcgc ccacttccac ggctcatgca
    30121 ccactttccc tggcacatcg cgcagttccg gtagccactg gcggataaac tcgccctcat
    30181 gatcaaattt ctcgccctgg gttgtcgggt tgaaaatacg aaaatacggc gctgcatcgg
    30241 ttccggttga agcggcccac tgccagccac cgttattggc tgccaaatca ccatcaatca
    30301 gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat caataaatct ttcaccagaa
    30361 aactggctgt aatcatccgt agcctgttat gcatccagcc agtgctgtta agctgacgca
    30421 tagcggcatc aacaatcggg tatcccgttt tgccttcctg ccaggcctgt aaatgtgcgg
    30481 gattgctctg ccactgtaca cgatccgtcc aggcaataaa tggacgatgt ttacacaacg
    30541 aggggtgata cgttatcagg tgacggtaaa actcgcgcca gatcagctca ttaagccaga
    30601 cactaccggc cccaccgtcc agcgcctgcg gctgttcagc caacaagcga tgcaagcact
    30661 ggcgaggcga taacccgccc gttgccaggc tggccgacaa acggctggtg ccttccactg
    30721 ccggaaaatc tcgttgttgc tcatattctc cggcaccgtt ctggcaaaac tggcgtaatt
    30781 gcgcaatcgc cgctttttct tccaccggaa aatgcgcagt atcgaaagac tgacgaggat
    30841 aattcagcgt aatggatggc gagggctcta tcgatccgct actacgaact tttggcgcag
    30901 cgacgcactc cggcatccct tcccgcagcc gtttcagcca ggcattctta aaaggcgtaa
    30961 agactttgta catctcgtga ttaccggtca tcaccgcgcc aggcggcagg atcacgctgt
    31021 catcaaatcc ttcacacacc acgttacgca gcgctctttc aacttccaca tcccgcgccc
    31081 gctcattcac ttcatactga tagttataaa acaggtgggt aacgctgttt tccgcgcaca
    31141 cctgtttaac tatttcgaca ctggcgacaa agtcatccac ttcacggaac aataaaggaa
    31201 tacctttttc cgcaagcgct atttgtagcc cattcagttg agcattgata agttcagcct
    31261 gacgcggcga catgttatgc gtcgcccact ggcgtggtgt agcgatatac aacgccagca
    31321 cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag attatcgtgc agacgtaaat
    31381 cctggcgaaa ccagaccaga tgggtagtca taaaactcct gaatggcgca agttaatcac
    31441 acgcggccaa agttcgaaat aacgctgccc tgaaagccag gcgtcaggat aaagcgccat
    31501 atagtgtttg atacgcatca gcggcgcaag aagtggttgc tcgccacggc gataactgtc
    31561 gataagcgca gccagctgct ggcgttctgt ggaatcaata tgcgggcgaa aataaccctg
    31621 aacgtgcatc aaaacattgg tgtgatcgcg cgggttggcg gggtgagaaa gcaaaacaac
    31681 tacgcgttgg cggtactggt tgtaatagtc atccagattt tgccactggt gaatatcagc
    31741 cacgaacggg ccaagtttgc ggtactccgg ctgagaatgc gccagaaaga cgagtttata
    31801 gcggctgtga taatcgagaa gcgccccgcg cgttaagcca tgttctttca gtcgatgtaa
    31861 ctcatgaagg gcaaagatac ggattagggt gctgtcatca aatcgttgta gattcattac
    31921 tgctcctgag cggtttacag gagtaagtgt agacgttgag aaatgatatt tgtgaagtta
    31981 atcctgttgt agcaggacaa atttacgaaa gcctgctccc cgcaaggact gacgtcaggt
    32041 agtttctaac catagctgcc ctttctattc aaacgtgaaa tcaataagct catcagggac
    32101 atcgcaaaca atatgaagac gggtttcttc tctgccgtga ctcttttcac taattgcgta
    32161 actgtcatgc ttgggattat ttaattcttt cagcggaaat ttttcctcga aggctttatt
    32221 tagccgcccc tggtttcctt ttacagcgaa taaataatca cctccctgtt tttgtatctt
    32281 ctctgcaata tctttctggc aacccatcgc atcggttttg atgatttttc ctttaatatc
    32341 cagcatgtta agaagttcag ggatagctgt gatctcattg gatttcttat ccgtcttgat
    32401 ctgtccgatg accagactgt gcattgttga gaacgcacta atgacatgaa tcgctccctt
    32461 acggcgactc ttgtcataag aatgccggtg tatttttcca tcaattgcga tgacgtcttt
    32521 atcatctgaa gaatggtagt caagcatcca gttaataaag ctctcatgaa atttcgcagg
    32581 acagatacag gatacaactc tggcaatggt atcgtgaaca ggaataccat tttcaaaatc
    32641 accatattgc ttcaaaa
//
LOCUS       DAJOSY010000047        32480 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJOSY010000047 DAJOSY010000000
VERSION     DAJOSY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.047"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(346..440)
                     /locus_tag="OHN27_002248"
     tRNA            complement(346..440)
                     /locus_tag="OHN27_002248"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(404..406),aa:Sec,seq:tca)
     gene            733..2115
                     /locus_tag="OHN27_002249"
     CDS             733..2115
                     /locus_tag="OHN27_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCQ2286682.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2125..4443
                     /gene="yicI"
                     /locus_tag="OHN27_002250"
     CDS             2125..4443
                     /gene="yicI"
                     /locus_tag="OHN27_002250"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCQ2286683.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(4496..6205)
                     /locus_tag="OHN27_002251"
     CDS             complement(4496..6205)
                     /locus_tag="OHN27_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCQ2286684.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(6326..7717)
                     /gene="xanP"
                     /locus_tag="OHN27_002252"
     CDS             complement(6326..7717)
                     /gene="xanP"
                     /locus_tag="OHN27_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCQ2286685.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7997..9202
                     /gene="gltS"
                     /locus_tag="OHN27_002253"
     CDS             7997..9202
                     /gene="gltS"
                     /locus_tag="OHN27_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCQ2286686.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9371..11452)
                     /gene="recG"
                     /locus_tag="OHN27_002254"
     CDS             complement(9371..11452)
                     /gene="recG"
                     /locus_tag="OHN27_002254"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCQ2286687.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDLALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVLFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11458..12147)
                     /gene="trmH"
                     /locus_tag="OHN27_002255"
     CDS             complement(11458..12147)
                     /gene="trmH"
                     /locus_tag="OHN27_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCQ2286688.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12154..14262)
                     /gene="spoT"
                     /locus_tag="OHN27_002256"
     CDS             complement(12154..14262)
                     /gene="spoT"
                     /locus_tag="OHN27_002256"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCQ2286689.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14281..14556)
                     /gene="rpoZ"
                     /locus_tag="OHN27_002257"
     CDS             complement(14281..14556)
                     /gene="rpoZ"
                     /locus_tag="OHN27_002257"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCQ2286690.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14611..15234)
                     /gene="gmk"
                     /locus_tag="OHN27_002258"
     CDS             complement(14611..15234)
                     /gene="gmk"
                     /locus_tag="OHN27_002258"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCQ2286691.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15492..17174
                     /gene="ligB"
                     /locus_tag="OHN27_002259"
     CDS             15492..17174
                     /gene="ligB"
                     /locus_tag="OHN27_002259"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCQ2286692.1"
                     /translation="MKVWMAILIGILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQQVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17171..17788)
                     /locus_tag="OHN27_002260"
     CDS             complement(17171..17788)
                     /locus_tag="OHN27_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCQ2286693.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(18079..18901)
                     /gene="dinD"
                     /locus_tag="OHN27_002261"
                     /pseudo
     CDS             complement(18079..18901)
                     /gene="dinD"
                     /locus_tag="OHN27_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
     gene            complement(19121..19984)
                     /locus_tag="OHN27_002262"
     CDS             complement(19121..19984)
                     /locus_tag="OHN27_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCQ2286694.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRSHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            20111..20827
                     /gene="rph"
                     /locus_tag="OHN27_002263"
     CDS             20111..20827
                     /gene="rph"
                     /locus_tag="OHN27_002263"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCQ2286695.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            20893..21534
                     /gene="pyrE"
                     /locus_tag="OHN27_002264"
     CDS             20893..21534
                     /gene="pyrE"
                     /locus_tag="OHN27_002264"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCQ2286696.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(21571..22167)
                     /gene="slmA"
                     /locus_tag="OHN27_002265"
     CDS             complement(21571..22167)
                     /gene="slmA"
                     /locus_tag="OHN27_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCQ2286697.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(22274..22732)
                     /gene="dut"
                     /locus_tag="OHN27_002266"
     CDS             complement(22274..22732)
                     /gene="dut"
                     /locus_tag="OHN27_002266"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCQ2286698.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(22710..23930)
                     /gene="coaBC"
                     /locus_tag="OHN27_002267"
     CDS             complement(22710..23930)
                     /gene="coaBC"
                     /locus_tag="OHN27_002267"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCQ2286699.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            24102..24770
                     /gene="radC"
                     /locus_tag="OHN27_002268"
     CDS             24102..24770
                     /gene="radC"
                     /locus_tag="OHN27_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCQ2286700.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            24987..25223
                     /gene="rpmB"
                     /locus_tag="OHN27_002269"
     CDS             24987..25223
                     /gene="rpmB"
                     /locus_tag="OHN27_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCQ2286701.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25244..25411
                     /gene="rpmG"
                     /locus_tag="OHN27_002270"
     CDS             25244..25411
                     /gene="rpmG"
                     /locus_tag="OHN27_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCQ2286702.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            25509..26318
                     /gene="mutM"
                     /locus_tag="OHN27_002271"
     CDS             25509..26318
                     /gene="mutM"
                     /locus_tag="OHN27_002271"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCQ2286703.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMNNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECKLLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(26357..26836)
                     /gene="coaD"
                     /locus_tag="OHN27_002272"
     CDS             complement(26357..26836)
                     /gene="coaD"
                     /locus_tag="OHN27_002272"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCQ2286704.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(26844..28121)
                     /gene="waaA"
                     /locus_tag="OHN27_002273"
     CDS             complement(26844..28121)
                     /gene="waaA"
                     /locus_tag="OHN27_002273"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCQ2286705.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            28534..29592
                     /gene="rfaQ"
                     /locus_tag="OHN27_002274"
     CDS             28534..29592
                     /gene="rfaQ"
                     /locus_tag="OHN27_002274"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCQ2286706.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKCFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDTLHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            29589..30713
                     /locus_tag="OHN27_002275"
     CDS             29589..30713
                     /locus_tag="OHN27_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCQ2286707.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYAQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVAELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIVEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            30706..31503
                     /gene="rfaP"
                     /locus_tag="OHN27_002276"
     CDS             30706..31503
                     /gene="rfaP"
                     /locus_tag="OHN27_002276"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCQ2286708.1"
                     /translation="MDELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            31519..>32480
                     /gene="waaO"
                     /locus_tag="OHN27_002277"
                     /gene_synonym="rfaI"
     CDS             31519..>32480
                     /gene="waaO"
                     /locus_tag="OHN27_002277"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCQ2286709.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLANSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNETLMRPVNSNYARYCAKHNFKQNKP"
BASE COUNT         7751 a         8045 c         8633 g         8051 t
ORIGIN      
        1 gaagtttata gtcagttttt ttcgccagcg gttaaagaaa tagaatagtc gtataaaaaa
       61 gggtgttctg gggcgagtaa aacaacatct tcaccttaat ggcgatacag ttggctgaga
      121 gtcagcaggg gggcaaatca cctgcaaata aacacggcgc atactggcgt gaatatcgcg
      181 gatgcgttcg aggacagaag aaccgacagg cggattcttc agccgctcat cgtccatcac
      241 aaaaccttag accaggtatt cctgaagccg attgaatatt gagcagtaga aaatatctgg
      301 attgacagta ttacaagggg ttggagaacg tcaggagagg cattttggcg gaagatcaca
      361 ggagtcgaac ctgcccggga ccgctggcgg ccccaactgg atttgaagtc cagccgcctc
      421 accggagacg acgatcttcc gcgcctcgat tgctacatgg aggcggggcg cattatagct
      481 acttccttga gtttctacat cccccagatc agttttttcc gcctaataca cgcagcgtat
      541 tatcacgaaa gcattatagt tcatataaaa cccatatttt acaatgatat atgagcggaa
      601 ttaacgctac cacgatcaca taaaacaaga atcataaatt aataaccaga tatcggaata
      661 ttcgctctca cagggatggt ttacaaaatg cgttatcggt ctcagcaccc ctatagcatc
      721 aaggaaaagc agatgaagag tgaagtgttg tccgttaaag agaaaattgg ttatggcatg
      781 ggagacgccg ccagccacat tattttcgat aacgtaatgt tatatatgat gttcttttat
      841 accgatattt ttggcattcc tgccggtttt gtcggaacca tgtttttggt cgctcgtgca
      901 ctggatgcga tttccgatcc ttgcatgggg ttgttggccg atcgaacgcg ctctcgctgg
      961 ggtaaatttc gtccgtgggt actgtttggc gcactgccat tcgggatcgt ctgtgtactg
     1021 gcctatagca cgccagatct cagtatgaac ggcaaaatga tctatgcagc aattacttac
     1081 accctactta ccttacttta taccgtcgtc aatatccctt actgcgcatt gggtggtgta
     1141 atcaccaatg acccgactca gcgtatctcg ctgcaatcct ggcgttttgt gctggcgacc
     1201 gcgggaggca tgctttctac tgttctgatg atgccactgg ttaatttaat tggcggtgat
     1261 aataaaccac tcggtttcca gggcggtatc gcggtccttt ccgtggtggc attcatgatg
     1321 ctggcatttt gtttcttcac cactaaagaa cgcgttgaag caccacctac aacaacgtct
     1381 atgcgggaag atttacgtga tatctggcaa aacgaccagt ggcggattgt cggtttacta
     1441 accattttca atatcctggc ggtgtgcgta cgcggtgggg cgatgatgta ttacgtcaca
     1501 tggattttgg gcacgccgga agtgtttgtc gcttttctca ccacttattg cgtgggtaac
     1561 ctgattggtt ccgcactggc aaaacctctg accgactgga aatgtaaagt cactatcttc
     1621 tggtggacga acgccctgct ggcagtgatt agcctcgcga tgttctttgt tcccatgcag
     1681 gccagcatca ctatgtttgt cttcatcttc gtgattggtg tgttgcatca actggtgaca
     1741 cctatccagt gggtaatgat gtccgatacc gtcgactacg gcgagtggtg caatggtaaa
     1801 cgcctgaccg ggatcagttt tgctggcacg ctgtttgtgc tcaaactggg gttggccttc
     1861 ggcggcgctc ttatcggctg gatgctggct tatggcggat atgatgcggc agaaaaagcg
     1921 cagaacagcg ccacgattag catcattatt gcgctattca cgattgttcc ggcgatctgt
     1981 tatttgctga gcgcgattat cgctaaacgc tactactcac tcacgacgca caatctgaaa
     2041 accgttatgg aacagctggc tcagggtaaa cgccgttgcc agcaacaatt cacctctcaa
     2101 gaagtgcaga actaaggaac ggcaatgaaa attagcgatg gaaactggtt gattcaacct
     2161 ggcctcaatt tgattcaccc gcttcaggtg ttcgaggttg aacagcagga taatgaaatg
     2221 gtggtctatg ctgccccccg tgatgtgcgt gaacgtacct ggcagcttga tacgccttta
     2281 tttacgctgc gctttttctc cccacaggaa ggtattgtcg gtgtgcggat tgagcatttt
     2341 cagggggcgc tgaataacgg tcctcattat ccgctcaata ttttgcagga cgtgaaggtc
     2401 acaatcgaaa acacagaacg ttatgctgag tttaaaagtg gcaacttaag cgcgcgtgtc
     2461 agcaaaggtg agttctggtc actggatttt ctgcgcaacg gcgaacgtat taccggtagt
     2521 caggtgaaaa ataatggcta cgtgcaggac acgaataatc aacgcaatta tatgtttgag
     2581 cggcttgatc ttggcgttgg cgaaacagtt tacggtctgg gagagcgctt tactgccctg
     2641 gtgcgcaatg gccagacggt agagacctgg aaccgggacg gcggcacaag tactgaacag
     2701 gcgtataaaa atatcccgtt ctacatgact aaccgtggtt atggggtact ggtcaatcat
     2761 ccccagtgtg tctcttttga agtgggatcg gagaaagtct ccaaagtgca gttcagcgtt
     2821 gagagtgaat atctcgaata ctttgttatc gacggcccga cgccgaaagc ggtacttgat
     2881 cgttataccc gctttactgg tcgtccggcg ctgccgcccg cgtggtcctt cggcctgtgg
     2941 ctaaccactt catttaccac caactacgac gaagcgacgg taaacagctt tatcgatggt
     3001 atggcggaac gcaatctgcc gctgcatgtt ttccactttg actgtttctg gatgaaagcc
     3061 ttccagtggt gcgattttga gtgggacccg ctgactttcc ctgacccgga agggatgatc
     3121 cgccgcctga aagcgaaagg gctgaaaatc tgcgtctgga ttaaccccta tatcggtcaa
     3181 aaatcccccg tctttaaaga gttacaagag aaaggctatt tactcaaacg cccggacggt
     3241 tcgctatggc agtgggataa atggcagcca ggtctggcga tttatgactt taccaatccg
     3301 gatgcctgca aatggtacgc cgacaaactg aaaggtctgg tcgcgatggg cgttgattgc
     3361 tttaagaccg actttggcga acgtatccca actgatgttc agtggtttga cggttccgat
     3421 ccgcagaaaa tgcataacca ttatgcgtac atctacaacg aactggtgtg gaacgtgctc
     3481 aaggacaccg ttggtgagga agaagctgtc ttgtttgccc gctcggcctc cgtcggtgcg
     3541 cagaaattcc cggtacactg gggtggcgat tgttacgcta actacgaatc aatggcggaa
     3601 agcctgcgcg gtggtttgtc tattggcctt tcaggttttg gcttctggag ccacgatatc
     3661 ggcggctttg aaaataccgc tccggcgcac gtttacaaac gctggtgcgc gtttggtttg
     3721 ctctccagcc atagccgttt acacggtagc aaatcttatc gtgtgccgtg ggcctacgat
     3781 gatgagtcct gtgatgtggt gcgcttcttc acgcaactga aatgccgcat gatgccgtat
     3841 ctgtatcgtg aagctgcgcg tgcgaacgcg cggggtacgc cgatgatgcg ggccatgatg
     3901 atggagttcc cggacgatcc ggcttgtgat taccttgacc gtcaatacat gttaggcgac
     3961 aacgtgatgg ttgcgccggt gttcactgaa gcgggcgatg tgcagttcta cctgccggaa
     4021 ggtcgctgga cacacctgtg gcacaacgat gaactcgacg gtagtcgctg gcataaacag
     4081 cagcacggct tcctgagtct gcccgtttat gtgcgtgata acactctact ggcgctgggc
     4141 aacaacgatc aacgtccaga ttacgtgtgg cacgaaggca cggcattcca cctctttaat
     4201 ctgcaagacg ggcatgaagc cgtctgtgaa gtgcccgctg ctgacggatc ggtgatcttt
     4261 actttaaaag cagcacgtac tggcaacacg attactgtga ctggtgcggg cgaggcgaag
     4321 aactggacac tgtgcctgcg caatgttgtg aaagtaaatg gtctgcaaga cggttcgcag
     4381 gctgaaagtg agcaggggct ggtggtgaag cctcaaggga atgcgctgac aattacgttg
     4441 taatctattg atggaatgcc gggtacgtct gagtcgtacc cggcgagatt gatgtttacc
     4501 gcaccggctc cgtcgatgta acttcaccgc tggaaacgat gccagcattc atggtttgag
     4561 tcacttgctg ctttgcggcg ttcacggcat gcaattgccc gctaaccgta ggtttcagcg
     4621 gggcattggc ctgaatatct ccactggcgg taagctgaat attgccgtcg ccagtcaacg
     4681 gtaattcagg ccatccccat tgttgcaaaa tattcaccgg cacaccgcgt ccattcaggc
     4741 tgatatgggt ctgacgttgt ggcgtttgtg aaacactggc agtggcttcc agaatgcctt
     4801 tttcagtaaa tgcactcagt tcgctgatat tcaccgtgct gctgttggcg gtcagcgcca
     4861 gcgacgggcg acgaacatca acacgattga atgtcgcggc ggcggcattc agattcgccg
     4921 agccactcca gacgccccat ttatgatcgg taaccagcgt caggttggca ccgtaaccat
     4981 cgagcgtggt gagctgccac gggaagtcag ggtcgatatc aatgatcaga ttgcggctgg
     5041 cgctaaatct cttcagttgc aggctgttta accaaccggg tgtcgtttcc atccacaact
     5101 gttgccagtt tttcggcaag gtatattcca gcccggcaat tgccgcatca tcaaggatca
     5161 acgttttccc gtcacgcagc cagttccctg acgttctgac cataccccct tcccagcggc
     5221 tggtgaactg gcgcagcgct acgccctgcg gggaaaattc cgcgtttata atcgggtcaa
     5281 ataaatgcag cgaaccataa atgaactcgc tagcgttcat cgacagtttg ccttcttgtg
     5341 tctgccagtc atctttactg aaggtcatgt tgcgcaagct gagatcgagg tcggtcaccg
     5401 cccagtccgg accttgcaaa cgagcatcga tcacttccag gcgaccaatc tgcaacgacg
     5461 ggacagagcg taatggcgca aagaagtcgg ttagcgattt ttcgctttgt agacggatat
     5521 ccgccatgcg caggttttcc acttgccagc tgccgtcggc gttacgctgc gcgtttccgg
     5581 ttaatgtccc gcgcgcgatg tcggcaccca ggttagtcag cgtaacgcga tcgttatcaa
     5641 tactgccttc aatcagtaca ttggtggcag gaacatcatt gagcgaaagc gatccggcac
     5701 taaactgaat ctgcgccttc gtacccagca ctttaccggc ttttggtgac cacggaacta
     5761 cgccgccatt tacccgctgc gcgctcagtt tccattcgct attcgggcta ttaaacgcca
     5821 tatcacgcag ttgcagacga tcggctttga acggtagcgg cgcggtctgg tcggtgagat
     5881 tcagcgtccc gttttccagc aggatggtat cgacatggcg tggttcggtc agttgccgac
     5941 tgcttagcgc aatgtcgaca cttttggcca ccagggtcgc gggctggcca tcacgaccaa
     6001 acgtgacgtt ctccagcacg atatgagatg gcgcggaaaa acggtgatcc atcgccccga
     6061 aggccagatg atagtcgcta ttctcggaaa cccatgcgct gatatgttct gctccccagc
     6121 gggtttgcag aagaaaatag aggccagcga tcgccactaa cagagcgatg agaatgtaga
     6181 gaagcagctt cccaataaat ttcatggtct tccatcccgt gaaatgcaca taagggagtt
     6241 atgcacgatt tacgcgcaat cctcaaggcg ggaatggtta aagaggtgag actgcggcgg
     6301 taattatctt accgccgcca gtgaattact gtttttctgg tgggaaaatc agattcagca
     6361 cgatggcagt aataccgccc gcggcgatcc cggaggagag caggtttttc agccattcag
     6421 gggcaaactg caaaatcagc ggctgctgag acacgcccag accaaccgcc agcgacagcg
     6481 cgataatcag aatcgcccga cggttcagcg gctcacgaga aacgatacgc acaccggagg
     6541 cggcgatggt gccaaacatt acaagcgttg cgccgcccag aaccggttct ggaatgtgtt
     6601 gtacaaaacc gctcactgcc gggaacagac ccagcacgat caacatcagc gcgacgacaa
     6661 aaccgacata gcggctggca acaccagtca actggatcac tccgttgttt tgcccgaagc
     6721 aggagttcgg gaaggtgtta aacaccgccg aaacaaacga gttcaggccg tttgccagca
     6781 cgccaccttt caggcgtttc atgtacagcg gaccggacac tggctgttcg gaaacgtcag
     6841 aggtcgccgt gatatcgcca atggtttcca gcgaagtgat cataaagacc agcatcagcg
     6901 gcagcagcag actccattca atgccaagac cgtaatagag cggcgttggc accataatca
     6961 gttcttgcgt catcggttcg ttgctttctg gcaacatgcc cataaaccac gccagcgcat
     7021 atccggccgc catcgcaatc accagtgagg ccacgcgtaa gtaagggtta cgttgacggt
     7081 taagcaggat aattaaggct aagaccacgc ctgccagcag cagatttttc ggtgcgccga
     7141 aggtgttatc gctcatggct gcgtaaccgc cgccaatgga cgttaaccca acctgaatta
     7201 gcgacaggcc gataatcatc accacaacgc cagaaaccag cggcgtaata atgcggcgcg
     7261 ccagatgcag aacgcgggag atcaccatct cggtgcaact tgccagcatc aacgtgccga
     7321 acaaagccgc catcatggta ggaacatcag caccaccggt tttcagcgcg gtaccgccca
     7381 taatcagcgg ggcaacaaag ttgaagctgg tgccctgaat agacaacagc ccggagccaa
     7441 ccggacccca ggccttaatt tgaataatcg atgccacacc ggaggcaaac agcgacatac
     7501 taataatgtg ttgcgtgtct tgtgccggta aacccagcgc ctggcagatt aatagcgctg
     7561 gcgtgatcac cgcaacgaac atcgccagca gatgctgaca ggcggcaaac agggtttgag
     7621 gaagcggcgg acgatcttca agacggtaaa tcagttcgct gttttgagtc tgcgcaaccg
     7681 gttgcgcatt ttctgactcg agggtggaaa cagacatcga aaacaatccc gtggtagcaa
     7741 agcgggcatt ttagctgacc gaaagagaaa agcaaacgtt tgccacatcc tcatctaaat
     7801 atttaaatga gataaatgca cttttttata taacttttgg tatttttctg cttaaaatcc
     7861 atgccatttg ttcattaaca gctgagaatt tgacccagga aagcataact cgatacccgt
     7921 catagttcat gaagttgcca ctgcgcgatg aagtatgacg agtatgaaag agtgatgcgg
     7981 atacaaagga gtaactatgt ttcatctcga tactttagca acgcttgttg ccgcaacgct
     8041 gacgttgctg ctcgggcgta agttggtcca ttccgtctcc tttttgaaga aatacaccat
     8101 accggaacct gttgcgggtg gtttgttggt ggcgctggcg ctactagtac tgaaaaaaag
     8161 catgggctgg gaagtcaact ttgatatgtc cctgcgcgat ccgttaatgc tggctttctt
     8221 cgccaccatt ggcctgaacg ccaacattgc cagtttgcgt gccggtgggc gtgtggttgg
     8281 catcttcttg attgtggttg ttggtctgtt ggtgatgcaa aatgccattg gcattggtat
     8341 ggctagcttg ttagggcttg atccgctgat ggggctgttg gccggttcta ttacgctttc
     8401 cggcggtcac ggtacgggcg ctgcgtggag taaattgttc attgaacgtt atggcttcac
     8461 caatgcgacg gaagtggcga tggcctgtgc aacgttcggt ctggtgctgg gcggcttgat
     8521 tggcggtccg gtggcgcgct atctggtgaa acactccacc acgccgaacg gtattccgga
     8581 tgaccaggaa gtcccgacgg cgtttgaaaa gccggatgtg ggacgcatga tcacctcgtt
     8641 ggtgctgatt gaaactatcg cgctgattgc tatctgcctg acggtgggga aaattgttgc
     8701 gcaacttttg gctggcactg cttttgaact gccgaccttc gtctgtgtac tgtttgttgg
     8761 cgtgattctg agcaacggtc tgtcaataat gggcttttac cgcgtctttg agcgtgcggt
     8821 atccgtgctg ggtaacgtaa gcttgtcgtt gttcctggcg atggcgttga tggggctgaa
     8881 actgtgggag ctggcttcgc tggcgctgcc gatgctggcg attctggtgg tacagaccat
     8941 cttcatggcg ttgtatgcca tcttcgttac ctggcgcatg atgggcaaaa actacgatgc
     9001 ggcagtgctg gctgcgggtc actgtggttt tggcctcggt gcaacgccaa cggcaatcgc
     9061 caacatgcag gcgatcactg aacgctttgg cccgtcgcac atggcgtttt tggtggtgcc
     9121 gatggtcggt gcgttcttta tcgatatcgt caatgcgctg gtaattaagt tgtatttgat
     9181 gttgccgatt tttgccggtt aaccgatgaa gcggcggtag aagtgccgcc gcaacaaaga
     9241 caaatgcctg atacgcttcg cttatcaagc ctgcgtattg attcataatt tattgaattt
     9301 gtagggtgat gaatcgcatc cggcaggaag gtagggtaac ctgaaatggc ggtcttctca
     9361 ctgccgcctt ttacgcattc gagtaacgtt ccgtctccgg catccagcgt tctatcaggg
     9421 cttttgcctg ttgtgggtaa cgttcgtgaa tatggcgtgc caggcgctga acttccggga
     9481 tcatcgcctg atcgcgcagt aaatccgcca ctttaaattc agcattaccc gtctgacgcg
     9541 tgcctaacaa ttcgccaggg ccgcgaatct ccagatcttt ttgcgcaatc acaaaaccgt
     9601 cgttactgtc gcgcagcact tgcaggcgaa tttgcgccgt tttagaaagc ggcgttttgt
     9661 agagcagcac gcagtgagaa gccaccgcgc cacgacctac gcgcccgcgc agctggtgta
     9721 actgcgccag acccagacgc tccgggtttt cgataatcat cagactggcg ttaggcacat
     9781 caacgccgac ttcaataacg gttgtggcaa ccagcaggtg tagctcacct tgtttaaacg
     9841 acgccatcac cgcctgtttc tcggcaggtt tcatccgccc gtgtaccagg ccaacgttca
     9901 actctggtag cgccagtttc aactcttccc aggtagcttc cgccgcctgc gcttccagca
     9961 attccgactc ttcaatcaac gtacaaaccc agtatgcctg acgaccttca gttatgcagg
    10021 cgtggcgcac gcggtcaata atgtcggtac ggcgggtatc aggaatagcg accgtagtca
    10081 ctggcgtgcg gcctggcggc agctcatcta tcaccgaggt atcgagatca gcatacgcag
    10141 tcattgccag cgttcggggg atcggcgtgg cggtcatgat caactgatga ggatggaagc
    10201 cttgctgctg gcctttctcc cataacgcca gacgctgatg cacgccaaaa cgatgctgtt
    10261 cgtcgataat caccagcgcc aggccgttaa acagcacctg ttcctggaag atggcgtgtg
    10321 taccgacaat catctgcacc tgaccgctgg cgatagcttc ctgctgtgcc agccgcgctt
    10381 tacctttctg cttaccggcg agccagccca cttcgatacc aagcggtgca aaccagttac
    10441 ggaagttatt ggcgtgctgt tcggcgagta attcggttgg tgccatcaat gccacctgtt
    10501 tgccgtgggc aatcgcacgc agcgcggcga gggcagcgac cagcgtttta ccggaaccta
    10561 catcgccctg taccaggcgc atcatcggca catccagcgc caaatcgcgc tcgatctccg
    10621 ccaccacgcg agcctgagcg cccgttggct tgaacggtaa ggcggcaaga agtttatttt
    10681 tcagcgtgtc attggcgctc agcggctggg catggaaacg ttgcgctccg gcgcgtaagg
    10741 ctaacatgct gaggttatgc gccagcagtt cttccagaat cagacgacgt tgcgccggat
    10801 gctgtccggt ttccagatcg ctaagctgca aagtcggtgg cgggcgatgc aaagtgcgca
    10861 acgcttctgg cagcgtcatc attccttgtg acagttccgg cggcaggagt tcttcaatgg
    10921 cgcaggtgtc gagcagatcc agcgcctggt cggttaattt acgcagcgtg gcctgcttta
    10981 cgccttccgt agttgggtaa accggcgtga gcgtttcctg taactctggc gtgctgagat
    11041 cgccttgcac gcggtattcc gggtggatca tctccgcacc atatttaccg cgctttgctt
    11101 ctccataagc cagtacacgg cggcccgccg ccaggctatt tttcattgcc gcgctgaaat
    11161 tgaaaaagcg catggtgagg atgccggaac cgtcgctgat ctggcaggtc atcatccgcc
    11221 gaccgccgaa ggagatattg cagttcagca cttcgccttc caccgtggca taaacgcccg
    11281 gcagtagttc tccgatgggg tagagatggg tgcgatcttc gtagcgcagg ggaaggtgta
    11341 agagtagatc ctgcacggta tgcaggttga ttttcgccag tttgttacta agtgctgcgc
    11401 caacgcccgt tagggaactg agtgggacag catctaacag gcgacctttc atggcactta
    11461 ccctgcagcc tgcatagtag cccaccagtc ggcatcagct tcgatctcgc cttgctgatt
    11521 gacgtgggga taaggcaggc ctttgcgttt tgcgactttc gccagcaccg gatagccgcc
    11581 ttcaaacaac aggcgttgtt gctctgcttc cggcaacatg ctgttttcac gcaggtacat
    11641 gcctgcattt tgccgctgac gctgggcttc gtaaagaatg agggctgagg caacggaaac
    11701 attcagcgac tgcaccatgc cgatcatcgg aatgatgatg tcctgatccg ccagggccaa
    11761 tgcttcctgc gtgatgcccg ttttctcctg tcccatcaaa atgcaggtcg ggcgagtgta
    11821 atcaatttcg cggaaatcga cagcgttatc agaaagatgg gttgccagaa tctgcatgcc
    11881 ctggcctttg agatgagcga cggcatcgcc aatggtgcgg tgtgttttca cctgtaccca
    11941 gctgttacta cccgccgctg ccgaagccat ggtgcgcatg cggctaccag gccagacggc
    12001 gtgaacttca tgtacgccaa cggcatctgc ggtacgaata atcgcagaaa cgttatgagg
    12061 tttgtggacc tgctccatgc agacggtcag atcaggctgc cgcctggcga gcatttcgca
    12121 gatgcgtgca taacgtgttg ggttcataaa acattaattt cggtttcggg tgactttaat
    12181 cacgtctggc atcacgcgga ttttgcgcat gatattcgcc agatgcacac ggtcacgagc
    12241 ggtcagacga ataaaggcgc tgtagacgcg accatctttc tcttccgtat tcaaactttg
    12301 aatattcgaa gtcgtggtgt taattgccgc cgtcaggttt gccagcgcac cctgatgatt
    12361 gaacatctcc accttgattt cggtgatgaa ctcctgcgcc gtctctttat cccattccac
    12421 agccataaac ttctctggct ctttctggta gccacggata ttacggcagg attcatggtg
    12481 gatcaccaga cctttaccgg ggctgacgtg ggcgataatc gggtcgccag gaatagggcg
    12541 gcagcatttc gcaaaggtga tcagcacgcc atcggcacct ttaatgggca gatgtccgtg
    12601 gctttgggtt gccggtggaa tggaggcgtc cccatgttgc agatttttcg cgaccaccac
    12661 gctcattgcg ttaccaagtc cgatttctgc cagcagatcg tcaagcgttg ccagcttcat
    12721 gcgatccagc tcgcgctgaa tattttcctg cgggatttca ttcagcttac ggctaccacc
    12781 caaagcatgg ttgagcagac gacggcccag gcttacagaa tcatcacgct tgaggttttt
    12841 cagcaactga cgaattttgg cgcgcgcttt cgagctaacg acaaagttca gccacgcggc
    12901 attcgggcga gcgcccggag cggtaatgat ttcaacggtt tgaccgctgg taagcggctg
    12961 cgacagcggg taaggctggc ggtcaacgcg tgcgcccacg caggcatggc cgatatcggt
    13021 atgcactgca taagcgaagt cgacgggcgt tgcaccggca ggcagctcga caatgcgccc
    13081 ttccggtgtg aaaacgtaaa tctcatccgg gaagagatcg gatttaacgc tctcgataaa
    13141 ttcaaacgaa ctaccggcgc tctgttgcag ctccagcagg ctttgcatcc agcgctgggc
    13201 gcggatttgt gcggtagtac tggtttcgcc gtgctcttta taagcccagt gcgcggcaac
    13261 acccatctcc gccatctggt ccatatcttc ggtacggatc tggacctcaa ccggcacacc
    13321 gtgcgggccg atcatcgagg tgtgcaaaga ctgatagccg ttcgcttttg gaatggcgat
    13381 atagtctttc acgcggcccg gacgcggctt gtacaggctg tgcatctggc ccagcacgcg
    13441 ataacaggtg tcagaatcat tgacgatcac gcggaaagcg tagatgtcca tgatcgagtg
    13501 aaaacgctgc tctttgagca ccattttgca gtaaatcgaa taaagatgct tctcgcgacc
    13561 actgacgcgg cacggtattc ccgcttcctg caaacgccct tcgatttcag aaagaatctt
    13621 ctggatcatc tctttacggt tgccgcgcgc ggctttcacc acttctttga ttacgcgata
    13681 acggttggga tacagcgcct caaaacccag ctcttcgagt tcggttttaa tgtggtggat
    13741 acctaaacgg tgcgccagcg ggctataaat ttcgagagtt tcacgggcga tgcggcgacg
    13801 tttgtccggg cgaagtgagc ccagcgtgcg catgttgtgg gtacggtcgg caagtttgat
    13861 gaggatgacg cggatatcct gcaccatcgc cataatcatc ttgcgaaagt tttcggcctg
    13921 cgcctctttc ttatcgcgga acttgagttt atcaagtttc gacaccccct ctaccagctc
    13981 ggcgacgctt ttaccaaaaa gctgttccat atcctggtag gtggcgggag tatcttcaat
    14041 cacgtcatgc agcagcgccg ccatcagcgt ttcatagtcg agtttcatct cggccagaat
    14101 gcaggcaacc gctaccgggt gcgtgatata gggttcaccg cttgaacgtg tttgcccctc
    14161 gtgagcatca cgtgcaacga gatacgcctg ccgcagacgc ttgatttggt cttccggcag
    14221 gtaggtttga atcagttgat tcaggctttc aaacagatac aagggcgacc cgctttgtga
    14281 ttaacgacga ccttcagcaa tagcggtaac ggcttgtaat tcagcggctt cctgctcttg
    14341 ctgttcctgg cgttcgcgaa cgtcgaggat ctggttgttg atcagacctt cttcgatttc
    14401 gcgcagcgcg attacagtgg ttttatcgtt ttcttccggt accagcggat cctttccgcc
    14461 tacctgcatc tgacgagcgc gacgcgcggc gaccagtacc aggtcaaaac ggttaccaat
    14521 tttctctaca gcgtcctgaa cagttacgcg tgccatactt aaaaagctcc acaggtgaag
    14581 aaatgactgg gcatgatact gaaatcaggt tcagtctgcc aacaatttgc tgattaaagc
    14641 gtcatgacgc tgcttttggc ggctcatgcg cagacgttcg gcgcgaataa tggtcttcaa
    14701 atcggtcaac gcggtatcga agtcatcatt cacaatcaga taatcatatt cggcgtaatg
    14761 gctcatttct gcaacagctt gcgccatacg ctttgcaatg acctcttcgc tgtcctgacc
    14821 gcgaccgcgt agacggcggt ccagttcaat tttggacggc ggtaaaataa agatactccg
    14881 cgcgtgcggc atcttctggc gaatttgctg cgcgccctgc cagtcgatat cgagaaaaac
    14941 atcgacaccg gtcgccagta cttgctcaat ggcctcacgc gaagtgccat agtaattacc
    15001 aaaaacttct gcgtgttcga ggaacgcatc tctgctaatc atttctttaa attcatcatg
    15061 attaacaaag aaataatgtt caccgtggac ttcaccagga cgcggttggc gtgtggtgtg
    15121 tgaaacagaa acctgggtgt catacaacgg ttgggttttt aataaagcct gaatcaggct
    15181 ggatttaccc gcgccactgg gggcagaaac aatataaagc gtgccttgag ccatgagtat
    15241 ctttcgtatg tgattagcga aataaagcct acatacgagc ttattataca cggcgcggcc
    15301 atgtgacgta gcccttgctc acactttttg cgcaaagttg ttgcgttttg tgtagctgct
    15361 tccgcatttg cctgtcattc actgcaataa agacgatttt caggaagcgt cctcgcagac
    15421 tccgtttttg ccactcggct ttgctttcat cacgttgtat atctgcctga ctatctacag
    15481 cggaggtgat gatgaaagta tggatggcga tattaatagg tatcttgtgc tggcaatcat
    15541 ctgtgtgggc ggtctgtccg gcctggtcgc cagccagagc acaggaagaa atttcccgcc
    15601 tgcaacagca aataaaacag tgggacgatg actactggaa ggaaggaaaa agtgaggtgg
    15661 aagacggtgt ttacgatcag ttaagcgccc gtcttacgca gtggcaacgc tgctttggga
    15721 gcgagccccg cgacgtgatg atgccgcctt taaatggtgc ggttatgcat ccggttgccc
    15781 atacgggtgt acgcaaaatg gtggataaaa acgcattaag tctgtggatg cgagaacgta
    15841 gcgatctttg ggtgcagcca aaagttgatg gcgttgcggt aaccctggtt tatcgggacg
    15901 ggaaactgaa caaagcaatc agtcgcggta atggcctgaa aggcgaggac tggacgcaga
    15961 aagttagctt aatttccgct gtgccgcaaa ccgttagcgg gcctttagcc aacagtacgc
    16021 ttcaggggga aatatttctc cagcgcgagg ggcatatcca acaacaaatg gggggaataa
    16081 atgcccgcgc aaaagttgct ggcctgatga tgcgccaggg gaatagcgac acgctaaact
    16141 cactggctgt ttttgtctgg gcatggccgg atggaccgca gttaatgacc gatcgtttaa
    16201 aggagctggc taccgcaggt tttactctaa cgcagaggta tacccgtgcg gttaaaaatg
    16261 ctgatgaggt tgcgcgcgta cgcaatgagt ggtggaaagc gaaattaccc ttcgtcaccg
    16321 atggcgtggt tgtgcgaggg gctaaggaac ccgaatcccg tcattggcta ccgggccagg
    16381 cggagtggct ggtggcctgg aaatatcaac ctgtagctca ggttgccgaa gtgaaggcga
    16441 ttcagtttgc ggtgggtaag agcggtaaaa tatcggtggt tgcgtcactt gcacctgtca
    16501 tgctggatga taaaaaagtc cagcaggtga atattggttc cgtcaggcgc tggcaggagt
    16561 gggatattgc gcctggtgat cagatcctcg tcagccttgc cggtcagggt attcctcgca
    16621 ttgatgatgt ggtctggcgc ggtgcagaac gtacaaaacc gacaccacca gaaaaccgct
    16681 ttaactcgtt gacctgctac tttgcttctg atgtttgtca ggaacagttc atttcacgct
    16741 tagtctggct ggggtcaaaa caggttctgg ggctggatgg cattggtgag gccggttggc
    16801 gcgcgctgca tcagactcat cggtttgagc atatcttttc ctggctttta ttaacgccag
    16861 agcaattaca gaacacgccg gggatcgcga aaagtaaaag tgcgcagtta tggcatcagt
    16921 ttaatctggc tcgtaagcag ccttttactc gctgggtgat ggcaatggga ataccgctaa
    16981 cccgggcggc gcttaatgcc agtgatgaac ggtcctggtc gcaactttta tttagcacgg
    17041 agcagttctg gcagcagctg ccggggactg gatctgggcg cgccagacag gttattgaat
    17101 ggaaggaaaa tgcgcaaatc aagaagctgg gcagttggct ggctgcccag cagatcaccg
    17161 gttttgaacc ttagtgacct tcgtggctgt agtaaaaaac cggtaatccc agcttaagac
    17221 gtagcgccag taaacgggca aaaaagccga ataccagggt ggagataatc accacatcat
    17281 ggttagaaac atagtgttgc agcgcgatgt acagcacggc ggaggcaaat gagacaccgg
    17341 catacaactc tttctggaat accagtggga tgcgtttaca gaacatatcg cgcaaaacgc
    17401 cgccaaacac gccggtggtt accgccgcga caacggcaat aattgggccg tgccccatat
    17461 ccagtgcaac ctgtgcgcca atgatagaaa agaccaccag tccgagcgca tcgagcacca
    17521 gaaacacttt gcgcaggtaa ggcattacag gagcaacgat agtggtgagt actgcggcgg
    17581 tagcgacgat aatcacatat tccgggtgtt tgacccagcc aagcggatag tggcccagca
    17641 gaatatcgcg cactgaccct ccgccaattg cggtggcggt cgcaataata attacgccaa
    17701 atgtatccat gcggcgtcgc ccggccgcca gagcccccgt catggcttcg gcagtaatgc
    17761 caaccaaata caaaatgtgt aacagcatgg ttctctccct aaaaatctgt ctggcagggt
    17821 agcgatttgt gcgcattgtc acgattgaga ttttttaagg tgaggggaaa ttgataactt
    17881 gtagttatgt gaaatcgcta ttttctgtag cagagatgca ttcttctgac ttcttgtctg
    17941 agaaaatgca taagaaaaaa taggttaaaa ctgagggggg aaaagaaagg tgcaatattt
    18001 agccattgtg ttcactgcac ctggaccttg gcttacgcat cgggtgtctc aatcaacaaa
    18061 aagagttttt agtttctgct atttcttctc tgtaattttt acactgtttt ccaactgctt
    18121 gatgctgacc tgcggggtcg gcaactcctc aggcatggtt ccgccaagtt cctgaatggt
    18181 ttgcctcacc ttgctaccca cgtcaaagtg tgtggtatta gcttgctgtt ttgaattcac
    18241 gccatcccgc ttgagttttt cttctgtttg ggtagctcga aagagattag ccgccagttc
    18301 tgttgaaccc atatgatcca ggatcttctg accctttttc agccccttcc gctgataaat
    18361 agctttctga tctaatccac catacagccc cggtaaccat gattctgaaa tatggcgaaa
    18421 tcgatagctg ttgctacccc agcttgctgc gctgcctcaa ctaattgttt gttatgttct
    18481 ttcaattcat ttctcagaaa aagacgcttt tcgtcttcac gaagttgcct gaaagcttca
    18541 tcgtcagcca gctcctgccg tcgggtctgg atagcaaaat aagtttgtcc tgccgcaata
    18601 accggtttcg cagggtcgcc gttttgtact accaggtaac aggcatagcg ggagagatga
    18661 acatcttcaa gttctcgttg agccctgaac ctaatacaac cattttggtg gtttcaacga
    18721 aatggtcaga tgcagcttga ttgctggctt cgcacgcttg agtcgcgcgg gtcagtacct
    18781 tttgaaaact gcgccagtcc cgataatcca gtaacggtgc caattctctc gctgaccaaa
    18841 attcttgccc ttcagtgcca taatgccgta tctcttcaaa aggttgatga tgttcgttca
    18901 ttgcccactc catgtgatat cgattgcaaa gaaagtaaat ctgcaactgt atttatatac
    18961 agttgtgagt atctcacgtt ctcagagtgc tgggcaaaaa acatcgcaaa atctggagca
    19021 ggcacgcaaa acagaattga atgttgcggg gaagaaaaat gccgctctgt ttgagtggcg
    19081 gcatttgtga aggcggaaaa gtaagaattg gcgttacgag ttattcgatg ttctgaatct
    19141 gctcgcgcat ctgctcaatc aacactttca gctcgatggc ggagtttgtc acttcggcat
    19201 tgatagactt cgacgcaaga gtgttcgact cgcggttgaa ctcctgcatc ataaaatcca
    19261 gacgacgacc aaccgcttct tttttcttca gaatgttgta ggtctctttg acatgcgctt
    19321 cgaggcgatc cagttcttcg gcaacgtcaa ttcgttgtgc cagcagaacc agttcctgct
    19381 ccagacggtt gttttccagt tgcacctgag catcttccag cttcgcgacc agacgctcac
    19441 gctgccattg caggatttcc ggcatatggg agcggacttt gaccacttcg gcggtgacgc
    19501 cttccagacg ctgctcgatc aatgctttca gcgcctgacc ttcggtttcg cgcgcgacga
    19561 taaagtcgtc cagcgtacca tccaacgccg cgagaatttc agcggcaatc gcgtcgagat
    19621 cctgctcctg ggctgccatc acgcccggcc agcgtagaat atcaaccggg ttgatttccc
    19681 cttcgtcact ctgcattttt acccagttcg cggcagttac cagctgttta gccagttttt
    19741 cgttgaggat cagctcacct tgtgcgctaa catctggctc atagcgcagg gtacattcca
    19801 ctttaccgcg cgtcaggcga gaacgaatac gctcacgaac gacaggttca aggctacgga
    19861 actgctccgg cagacgaaag taagtttcca gataacgctg gtttaccgag cgcatttccc
    19921 aggttgcgct cccccattca cccttgattt cacgccgggc gtaggcggtc atactgcgga
    19981 tcatagacgt tcctgtttat aaaaggagag gtggaaggat tatagccatc gatgccttgt
    20041 caggatagga ataaccgccg gaagtccgta taatgcgcag ccacatttgt ttcaagccgg
    20101 agatttcaat atgcgtccag caggccgtag caataatcag gtgcgtcccg ttaccctgac
    20161 tcgtaactat acaaaacatg cagaaggctc ggtgctggtc gaatttggcg ataccaaagt
    20221 gttgtgtacc gcctctattg aagaaggcgt gccgcgcttc ctgaaaggtc agggccaggg
    20281 ctggatcacc gcagagtacg gcatgctgcc acgttctacc cacacccgta acgctcgtga
    20341 agcggcgaaa ggtaagcagg gtggacgcac aatggaaatc cagcgtctga tcgcccgtgc
    20401 tcttcgcgcg gcagtagatt tgaaagcgct gggtgagttc accattacgc tggactgcga
    20461 cgtgcttcag gctgatggtg gcacgcgtac cgcgtcgatt acgggtgcct gcgtggcgct
    20521 ggcagatgcg ctacagaagc tggtggaaaa cggcaagctg aaaaccaacc cgatgaaagg
    20581 gatggtagcc gcagtttctg tcggtattgt aaacggtgaa gcaatttgcg atctggaata
    20641 cgttgaagac tcagccgcag agaccgacat gaacgtagtg atgaccgaag acgggcgcat
    20701 cattgaagtg caggggacgg cagaaggcga gccgttcacc catgaagagc tactcacctt
    20761 gttggcgctg gcccgagggg gaatcgaatc cattgtagcg acgcagaagg cggcgctggc
    20821 aaactgattt ttaaggcgac tgatgagtcg cctttttttt gtctgtagaa aagtaagatg
    20881 aggagcgaag gcatgaaacc atatcagcgc cagtttattg aatttgcgct tagcaagcag
    20941 gtgttaaagt ttggcgagtt tacgctgaaa tccgggcgca aaagccccta tttcttcaac
    21001 gccgggctgt ttaataccgg gcgcgacctg gcactgttag gccgttttta cgctgaagca
    21061 ttggtggatt ctggcattga gttcgatctg ctgtttggcc ctgcttacaa agggatcccg
    21121 attgcgacca ctaccgccgt ggcgctggcg gagcatcatg accttgacct gccgtactgc
    21181 tttaaccgca aagaggcaaa agaccacggt gaaggcggca atctggttgg tagcgcgtta
    21241 caaggacgcg taatgctggt agatgatgtg atcaccgccg gaacggcgat tcgcgaatcg
    21301 atggagatta ttcaggctaa tggcgcgacg cttgctggcg tattgatttc gcttgatcgt
    21361 caggaacggg ggcgcggcga gatttcggcg attcaggaag ttgagcgtga ttacaactgc
    21421 aaagtgatct ctatcatcac cctgaaagat ctgattgcct atctggaaga gaagccggaa
    21481 atggcggaac atctggcggc ggttaaggcc tatcgcgaag agtttggcgt ttaaagaaac
    21541 tcgccggatg aaaagtcatc cggcgtcata ttactgcaac tgcgcggcaa ttagcggcca
    21601 gcgggcgtca aaatcatccg tcgggcggta tttaaattcg ctgcggacaa aacgtgacag
    21661 cataccttca cagaaggcca ggatctggct tgccagcagg gtttcatcgg tggtgtaacc
    21721 ttcaccctca cgcattctct tttcacgcaa tacctgacgc agctgcgctt caatacgctc
    21781 gaacagctgg ttgatacgcc cttgcaggcg atcctgttca aacatcagcg catgaccagt
    21841 gaggatgcgg gtcaggccag gattacgctc accaaaaccg agaagcagca acacaatcag
    21901 acgcaggcgc gctgtggtgt ctttctcatc tttcagaatc aggttgatgc gagtaatcag
    21961 gctatcttcg ataaactcaa tcaggctatc gaacatgcgg gtcttactgg ggaagtggcg
    22021 atacagtgcc gcttcggaaa cgccgacaga ggcggccagt tttgccgtcg tgatacgttg
    22081 gcttccatcg ctggattcca gcatcagcgc cagagactga agtatttcct cgcgacggtt
    22141 ccttttcgca gtttgttttt ctgccatgtt acaaaatacc cctgaaaata agcacttgcc
    22201 aggcggcacc cacgctatga ccgcaaacga aatgtttgcg gctatgttat gacgttattc
    22261 ggatgcgtat gtgttactga cgaccagagt gaccaaagcc gccttcaccg cggtcggtgg
    22321 cgtcgaaatc ttccaccaga ttaaattcag cctgtactac cggaacaaaa atcatctggg
    22381 cgatgcgttc gccaggttga atggtgaagc tgtcctgacc acggttccaa acggaaatca
    22441 tcaactggcc ctgatagtca gaatcgatca atcctaccag gttaccaagc acgataccgt
    22501 gcttatgtcc caatccggag cgcggcagca tcattgccgc cagtgaagga tcggcaatat
    22561 gaatcgccag cccggtcgga accagcgtag tgtcacccgg agccagttct acggcgtcgt
    22621 cgagacaggc acgcaggtca agtccggcag agccagaggt ggcataagtc gggagcggaa
    22681 attccttccc aacgcgcggg tccagaatct taacgtcgat ttttttcatc ataacgggtc
    22741 acgatctcgt cgagtaataa ttggccaagg agctctttgc gctcaagcgg taagacttta
    22801 tctccgtcct gccagaaaag gtgtaatgcg ttgttgtcgc tgttaaatcc ttgagttggc
    22861 tgggaaacat cgttcgcgca gatcagatca aggtttttac ggatacgttt ttgccgggcg
    22921 tattcttcca cattatttgt ttcggcggca aatccaacga cgtagggtcg atggtctttt
    22981 agtgcggcaa cgcctgcgac gatatcgggg tttttaacca tttttattgt taattcatca
    23041 ccctgcgtgg cctgcttttt gattttttct ggggccacgg tagctgcgcg ataatccgcc
    23101 acggcggcgc aaccgataaa aatattttgc tgctgtacag aagcattcac ggcggcttcc
    23161 atttccagcg cggtcatcac atcaacacgt ttaacaaacg gtggcgtcgg tagtgaaact
    23221 ggacctgata ccagcgtgac gttcgcgcca cgacgggcag cggcggcggc gatagcaaaa
    23281 cccatcttgc cggagctgtg attagagata taacgcaccg gatcgagcgg ttcacgcgtc
    23341 ggaccggcgg taatcataat gttcagatgt ttcaggtcgt tgacgggcga aaaatgcgcc
    23401 accgccatat ccacaatggt taacggatcg agcattcgcc caggaccgat atcaccacaa
    23461 gcctgactgc cactgtctgg cccccagatg agcaaaccac gggaagcaag cacctctaaa
    23521 ttatgctgcg tggcagcggc acggtacatc tgctggttca tggcggggag cacggctaca
    23581 ggcgcaggtg tagccagaca aatcgtcgat accaggtcat tcgccattcc ggcagcaaca
    23641 cgggcaatca aatctgccgt ggcaggggcg agaatcacta aatcagccca tttacccagc
    23701 tcaatatggc ccatagcggc ttcggctgcc gggtccagca aactgtcgga aacgggataa
    23761 ccagaaaccg cctgcaagct aagtggggtg ataaaggctt ttgccgcttc ggtcatggct
    23821 acgcggacgt cggccccgcg atcgcgcaaa cgacgcacca gttcaggggt tttataggca
    23881 gcaataccgc cgctaacgcc gagaacgatt tttttaccgg ccaggctcat gatgattttt
    23941 cctgttgggt gacaccagaa gttggcgatt ttatcacaat acttttgttg tcgtgccttc
    24001 acctgagaat cactttgcga ggcgctttcc aggattgaaa actggccgtc gatttaacgg
    24061 aacggctatg acaggatgcg agcaccacaa aggaggtgaa ggtgaaaaac aatgcacagc
    24121 tgttgatgcc gcgcgaaaaa atgctgaagt ttggtattag cgccttaacg gatgtcgagc
    24181 tgctggcgtt atttctgcgt accggaacgc gcggtaaaga tgtattaacc ttggcaaaag
    24241 agatgctgga gaatttcggc tctctttatg gcttgttaac ctctgaatat gaacaattta
    24301 gtggcgttca tggaattggc gtagcgaaat ttgcccagtt aaaggggatt gctgaactgg
    24361 cgcggcgtta ctacaacgtg cggatgcgtg aagaaagccc tttactcagc ccggagatga
    24421 cgcgggaatt tttacaaagc cagctcacgg gtgaggagcg ggagatcttt atggtgatct
    24481 ttctcgactc acaacaccgg gttataacgc atagccgtct tttttccggc acgctaaacc
    24541 atgttgaagt ccatcctcgg gaaattatcc gcgaagcgat aaaaataaac gcctcggcgc
    24601 tgatccttgc acataatcac ccttcgggtt gtgctgaacc cagtaaagcg gataaactca
    24661 ttactgaacg gataataaag agttgtcagt tcatggattt acgcgtgctc gaccatatcg
    24721 tgattgggcg tggagagtat gtttcttttg ccgaacgcgg ctggatttaa cccgctatgc
    24781 gcgatccttc gggatctttg tctgttcggg acttgagcac atcgctgagt cagcgtatac
    24841 tacgccacct ttgagaatct cgggtttggc atttgggcct ggcaatcgag agttcacata
    24901 gaactgcgat gaccgggctg taaagcctga cgaggcgcca ataccccata cgaagctcga
    24961 gctaatttga tttttggaga atagacatgt cccgagtctg ccaagttact ggcaagcgtc
    25021 cggtgaccgg taacaaccgt tcccacgcac tgaacgcgac taaacgccgt ttcctgccga
    25081 acctgcactc tcaccgtttc tgggttgaga gcgagaagcg ttttgtcacc ctgcgcgtat
    25141 ctgctaaagg tatgcgtgta atcgataaaa aaggcatcga tacagttctg gctgaactgc
    25201 gtgcccgtgg cgaaaagtac taagtactta gaggaaataa atcatggcta aaggtattcg
    25261 tgagaaaatc aagctggttt cttctgctgg tactggtcac ttctatacca ctacgaagaa
    25321 caaacgtact aagccggaaa aactggaact gaaaaaattc gatccagttg ttcgccagca
    25381 cgtgatctac aaagaagcga aaatcaaata attctcgctt tgatgtaaca aaaaacctcg
    25441 ctccggcggg gttttttgtt atctgcttgc ccccatattg actgcatctg ttcattcctg
    25501 gagatgctat gcctgaatta cccgaagttg aaaccagccg ccgcggcata gaaccgcatc
    25561 tcgttggtgc aaccattctt catgcagtgg tgcgcaacgg acgcttgcgc tggccggttt
    25621 cagaagagat ctaccgttta agcgaccaac cagtgcttag cgtgcagcgg cgggctaaat
    25681 atctgctgct ggagctgcct gagggctgga ttatcattca tttagggatg tctggcagcc
    25741 tgcgcatcct tccagaagaa cttccccctg aaaagcatga ccatgtggat ttggtgatga
    25801 acaacggcaa agtgctgcgc tacaccgatc cgcgccgctt tggtgcctgg ctgtggacca
    25861 aagagctgga agggcataat gtgctggccc atcttggacc ggagccgctt agcgacgatt
    25921 tcaacggtga gtacctgcat cagaagtgcg cgaagaaaaa aacggcgatc aaaccgtggc
    25981 tgatggataa caagctggtg gtaggcgtag ggaatatcta tgccagcgaa tctctgtttg
    26041 cggcggggat ccatccggat cggctggcgt catcactgtc gctggcggag tgcaaattgc
    26101 tggctcgggt gattaaagcg gtgttgctgc gttcgattga gcagggtggt acaactctga
    26161 aagattttct gcaaagtgat ggtaaaccgg gctatttcgc gcaggaattg caggtttacg
    26221 ggcgaaaagg cgagccgtgt cgggtgtgcg gtacgccgat tgtggcgact aaacatgcgc
    26281 agcgggcaac gttttattgt cggcagtgcc agaagtaatt catgcgcgcc ggatggcata
    26341 ccatccggca taaacgctac gctaacttcg ccatcagcgc ctgatggaca ttctccggca
    26401 ggaaatgggt gacatcgccc tgatggcgcg ccacctcttt caccaacgat gaagagataa
    26461 acgaccactc tttcgacggc atcagaaaca cactttccag ttccggcatt aagtggcgat
    26521 tcatatgcgc cagctgcatt tcatattcaa aatccgccac cgcacgcagg ccacgaatca
    26581 gcaccgtagc gtgttgatta cgggcgaagt tcgccatcaa atcactaaac ccgaccactt
    26641 ccacgttccc cagatgcgcg gttgcctgct gtgccagctc cacacgctct tccagggtaa
    26701 acatcggttt tttactgggg ctggcggcaa tcgccagaat aacgtgatcg aacatttgcg
    26761 tggcgcgcgt cacgatatcg atatgaccat tggtaatggg atcgaaagta cccggataaa
    26821 tcgcccgttt ttgcataaca acctcaatgc gttttcggtg gcaggtaagg ttccagcagt
    26881 tgaagcagac gctgtagcgc gccctggttt tgatacagta cttcaacggc atgacggcca
    26941 tagaaactac ggtaatcggc gtcggtgagt aaagaggaaa cctcttttgc aagcgacgtg
    27001 gcatcggtaa cggtaatcag cccgcttgcc tgctccagcc gcgcgcaaat gtctttaaag
    27061 ttaaaagtat gcggccccat caataccgga atagcgtgtg cggcagcttc cagcggatta
    27121 tgcccaccac gttcaaccag tgaaccgcca acaaaggcga gatcggcaat gccgtacagt
    27181 aacatcaact cgcccatcgt atcgcccacc acaacctgcg tgctggtgga ggggacttcc
    27241 cctgaagagc gtgtgatata gcttagtcca gcctggcgga caagattaat cgcatccggg
    27301 aagcgttctg gatgacgggg taccaggatg agcaataaat tcgggaattg ctgtaacaat
    27361 gcctgatgtg cggcgatcac cacactctct tcgccttcgt gagtgctggt ggcaatccat
    27421 accgggcggt gtggtgccca ctggcggcgc agcgtcacgg ctttagcagc caactgcggc
    27481 gttacagaaa tatcgaattt caggctaccg gtaacggtta cctggttatt ttttgcgccc
    27541 agcgccacaa aacgtgcacc atcttcttca ttttgggcgg caatcagcgt aatacgacgc
    27601 agcaagcgac ggacgaattt acccagtttg gcataacctg cggccgagcg ggcagagagt
    27661 cgcgcgttag cgatcaccag cggaatttta cgtttatgta gcgccgcaat caggttaggc
    27721 catagttcgg tttccataat caacaccagt ttagggtcga ctttattcag gaaacggttg
    27781 agcgcatcgg gcaggtcata cggcagataa acgtgctgaa catccttccc gaaagccgat
    27841 tgtacgcgct ccgaaccggt tggcgtcatg gttgtaacgg taatcggtaa atcaggataa
    27901 cgatgacgca gcgcgcgcac caacgggatc gccgccagag tttcaccgac ggagacggag
    27961 tgcagcataa tgccgcctgg ttttagcgga tggcggtaaa aaccgtaacg ttcaccccag
    28021 cgttttcgat aggccggagc cttacgtccg cgcacccaga gccgtatcca gatcagcggc
    28081 tgaataaggt agagaagggc ggtgtaaagc aattcgagca tagtaaatag ctgacttatg
    28141 gatgtgctgg ggattctatg tatttagctg tggctttacc attacttttc ccgtttttga
    28201 cttaaatagc aatttacccg ccaattcttg ctgaacatct atactttttt tgtattttta
    28261 tgcgattcat tgatttctgc tgtacgtgca ggaaggaatc gcagagcaac agtgaaagaa
    28321 tatggttgct aatgatttta tttgattgat ttctttattg aaaatcaaca atgtgcttaa
    28381 aaatcattcg ggtcggattg ccgcgaaaga agtgatacac tagcgcgtca atatcagtgc
    28441 ggtatcagta ttcaggtagc tgttgagcct ggggcggtag cgtgcttttt ctgcttcacc
    28501 ttaatcggat aatctcaaca aaaagagtta cttgtggata agccatttcg aagaattttg
    28561 ctcattaaga tgcgttttca tggggatatg ttattaacta ctcccgtcat tagttcgctg
    28621 aaaaaaaatt accctgacgc aaaaatcgat gtgctgcttt atcaggacac catcccgatc
    28681 ctgtctgaaa atccagagat taacgcgctc tacggcataa aaaataaaaa agcaaaagcc
    28741 tcagaaaaaa ttgccaactt ttttcatctc atcaaggtat tacgtgccaa taagtatgac
    28801 cttatcgtca atcttaccga tcaatggatg gttgctatac tggttcgctt attaaatgct
    28861 cgtgtgaaaa tttcccagga ttatcatcat cggcagtctg ctttttggcg taaatgtttc
    28921 acccatttgg tgccgttgca gggtggaaat gtggtggaaa gtaacttatc cgtgctgaca
    28981 ccattgggac ttgattcgtt ggtgaagcag acaaccatga gttacccgcc tgcaagctgg
    29041 aaacgtatgc gtcgcgaact tgatcacgct ggtgttggac aaaattatgt ggttatccaa
    29101 cctacggcgc ggcaaatctt caaatgctgg gacaacgcca agttttccgc tgtgattgat
    29161 accttacatg ctcgtggtta tgaagtcgtt ctgacgtccg gcccagataa agacgatctg
    29221 gcctgcgtca atgaaattgc gcagggatgc cagacgccac cagtaacggc gctggctgga
    29281 aaggtgacct tcccggaact tggtgcgtta atcgatcatg cgcagctgtt tattggcgtt
    29341 gattccgcac cggcgcatat tgccgctgca gttaatacgc cgctgatatc gctgtttggt
    29401 gcgacagacc atattttctg gcgtccctgg tcaaataaca tgattcaatt ctgggcggga
    29461 gattaccggg aaatgccaac gcgcgatcag cgtgaccgaa atgagatgta tctttcggtt
    29521 attccggcgg cagatgtcat tgctgctgtc gataaattac tgccctcctc cacgacaggt
    29581 acgtcgttat gatcgttgct ttttgtttgt ataaatattt tccttttggc ggtttgcagc
    29641 gcgattttat gcgtattgct cagacagtcg ccgcccgagg tcatcatgtt cgggtttatg
    29701 ctcagtcgtg ggaaggcgaa tgtcctgatg tatttgaact gatcaaagtg ccggttaaat
    29761 cgcataccaa tcacgggcgc aatgcggagt attttgcctg ggtgcaaaaa catttacgcg
    29821 aacatcccgt cgataaagtc gttggattca acaaaatgcc ggggctggac gtttattatg
    29881 ccgctgatgt ttgttatgcc gagaaagtag cgcaggaaaa aggctttttc tatcgcctga
    29941 cgtcacgtta tcgccattat gccgcctttg agcgggcaac cttcgaacag ggcaagccga
    30001 cacagctgct gatgctgaca gataagcaaa tcgccgattt ccagaaacat tatcagactg
    30061 aagcggagcg ttttcatatt ctgccaccgg ggatttatcc tgatcgtaaa tatagccagc
    30121 agccagccaa tagccgtgaa atcttccgta agaagaatgg aataaccgaa caacaatatt
    30181 tattgttgca ggtcggttca gacttcacgc gtaaaggtgt cgatcgttcc attgaagcac
    30241 ttgcttcgtt accggattcg ctgcgccaca acacattgct atatgttgtt gggcaggata
    30301 aaccgcgaaa atttgaggcg ctggcagaaa aacgcggcgt gcgcagtaat gttcacttct
    30361 tctcggggcg caacgatgtc gcggaattaa tggcggcggc ggatttatta ctgcatcctg
    30421 cctaccagga agcggcggga attgtgctgc tggaagcgat tactgcagga ttaccggtac
    30481 taacaactgc cgtttgtggc tatgcgcatt atattgtcga cgctaattgc ggcgaggcta
    30541 ttgttgagcc attccgccag gaaacattga atgagatttt acgcaaagcg ttaacgcaat
    30601 cttcattgcg ccaggcctgg gcggaaaatg cgcgacatta tgctgataca caagatttat
    30661 acagtctgcc agagaaagcg gcggatatca taacgggtgg tctggatgga tgaacttaaa
    30721 gagccgtttg ccacgttatg gcgcggcaaa gatccttttg aggaagttaa aaccttgcag
    30781 ggtgaggtat ttcgtgaact ggaaactcgc cgtactctgc gctttgaaat ggcgggcaaa
    30841 agctattttc tcaaatggca tcgcggcacg accctgaaag agataatcaa aaatttactc
    30901 tcattgcgga tgccagtatt aggcgcagac cgcgaatgga atgcgattca tcgactgcgg
    30961 gatgtcggcg ttgatactat gtatggggtg gcatttggcg aaaaaggcat gaatccgctg
    31021 accagaactt catttattat taccgaagat ctgacaccaa ccataagtct ggaagattac
    31081 tgtgctgact gggcgactaa ccctccagat gttcgcgtaa agcgtatgct tattaagcgt
    31141 gtcgcgacga tggtgcgcga tatgcatgct gcgggcatta accaccgtga ctgttatatc
    31201 tgtcatttcc tgctgcactt gcctttttcc ggtaaggaag aggagttaaa aatttcggta
    31261 attgacctgc accgggcgca gcttcgcacg cgcgttccac gtcgttggcg cgataaagat
    31321 cttattgggc tttatttttc atcgatgaat atcggcctga ctcagcggga tatctggcgg
    31381 tttatgaaag tgtattttgc cgccccgctt aaagacattc tcaagcagga acaaggactg
    31441 ctgtcgcaag cagaagcaaa agccacaaaa atcagggaaa gaacgattcg aaaatcgttg
    31501 taatttccgg agagaaatat gagtgcccac tattttaatc cacaagagat gatcaataag
    31561 acaatcatct tcgatgaaag gccagcggcg tcagtggcat catcattcca tgttgcttat
    31621 ggcattgata aaaactttct ttttggttgt ggtgtttcaa tcacgtcagt tttgttacat
    31681 aacaacgacg tgagttttgt tttccacgtt tttattgatg atatccctga agccgatatc
    31741 cagcgtttag cccaattggc gaatagctat cgtacctgta tccagatcca tctagtaaat
    31801 tgtgaacggc ttaaggcatt accgacgacc aaaaattggt ctattgccat gtatttccgt
    31861 tttgtaattg cagattactt tattgatcaa caagataaga tcctttacct ggatgctgat
    31921 atcgcctgtc agggaaactt aaagccgctg ataacaatgg atcttgccaa taacgttgct
    31981 gctgttgtta ctgaacgcga tgctaactgg tggtcgttac ggggtcaaag tctgcagtgt
    32041 aatgaacttg aaaagggtta ctttaattca ggtgtcctgt taattaatac actagcgtgg
    32101 gcgcaggagt ccgtttctgc taaagcgatg tcgatgcttg ctgataaagc catcgtttcc
    32161 cgtttaacct atatggatca agatattctt aatcttatcc tgttagggaa agttaaattc
    32221 attgatgcta aatacaatac gcaatttagt ttaaattatg aattaaaaaa atcatttgtt
    32281 tgtccaatta atgatgaaac cgtattaatt cattatgtcg gcccgacaaa accctggcat
    32341 tactgggccg gttatccaag tgcgcaacct tttatcaaag ccaaagaagc atcgccctgg
    32401 aaaaatgaaa cgttaatgcg gccagttaac tcaaactatg ctcgttattg cgccaagcat
    32461 aattttaaac aaaataaacc
//
LOCUS       DAJOSY010000048        32194 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJOSY010000048 DAJOSY010000000
VERSION     DAJOSY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.048"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(289..774)
                     /gene="sixA"
                     /locus_tag="OHN27_002278"
     CDS             complement(289..774)
                     /gene="sixA"
                     /locus_tag="OHN27_002278"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCQ2286710.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(977..3121)
                     /gene="fadJ"
                     /locus_tag="OHN27_002279"
     CDS             complement(977..3121)
                     /gene="fadJ"
                     /locus_tag="OHN27_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCQ2286711.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGAGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3121..4431)
                     /gene="fadI"
                     /locus_tag="OHN27_002280"
     CDS             complement(3121..4431)
                     /gene="fadI"
                     /locus_tag="OHN27_002280"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCQ2286712.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDFNKARIMRQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4611..4895)
                     /locus_tag="OHN27_002281"
     CDS             complement(4611..4895)
                     /locus_tag="OHN27_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCQ2286713.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5267..6607
                     /gene="fadL"
                     /locus_tag="OHN27_002282"
     CDS             5267..6607
                     /gene="fadL"
                     /locus_tag="OHN27_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCQ2286714.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6973..8031
                     /locus_tag="OHN27_002283"
     CDS             6973..8031
                     /locus_tag="OHN27_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286715.1"
                     /translation="MLPSISINNTSAAYTESINENNNDEVNGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRGNSNPERININNSAVTYIDIGSNDTDHITISIDNQEPIKLPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNDVFKRYGRA
                     IRYDFQVDDAKYKCDHLKEIVSTLVGNKINVGHSQKIYKHFKDLEGKIEERLQNRQAE
                     YQNEINQPSAPGVNFDDI"
     gene            complement(8213..8968)
                     /gene="mlaA"
                     /locus_tag="OHN27_002284"
     CDS             complement(8213..8968)
                     /gene="mlaA"
                     /locus_tag="OHN27_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCQ2286716.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            9262..10194
                     /locus_tag="OHN27_002285"
     CDS             9262..10194
                     /locus_tag="OHN27_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCQ2286717.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLIRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNYKNPA"
     gene            10270..10344
                     /locus_tag="OHN27_002286"
     tRNA            10270..10344
                     /locus_tag="OHN27_002286"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10303..10305,aa:Arg,seq:cct)
     gene            complement(10440..11375)
                     /gene="dsdC"
                     /locus_tag="OHN27_002287"
     CDS             complement(10440..11375)
                     /gene="dsdC"
                     /locus_tag="OHN27_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HCQ2286718.1"
                     /translation="MEPLREIRNRLLNGWQLSKMHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHHFLMDEEILPVCSPEYAQRHALTNTVINLCHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
     gene            11593..12930
                     /gene="dsdX"
                     /locus_tag="OHN27_002288"
     CDS             11593..12930
                     /gene="dsdX"
                     /locus_tag="OHN27_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HCQ2286719.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSVD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYILVEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTFLLSFII"
     gene            12948..14276
                     /gene="dsdA"
                     /locus_tag="OHN27_002289"
     CDS             12948..14276
                     /gene="dsdA"
                     /locus_tag="OHN27_002289"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCQ2286720.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(14384..15922)
                     /gene="emrY"
                     /locus_tag="OHN27_002290"
     CDS             complement(14384..15922)
                     /gene="emrY"
                     /locus_tag="OHN27_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCQ2286721.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIVVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIIMPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15922..17085)
                     /gene="emrK"
                     /locus_tag="OHN27_002291"
     CDS             complement(15922..17085)
                     /gene="emrK"
                     /locus_tag="OHN27_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCQ2286722.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            17501..18115
                     /gene="evgA"
                     /locus_tag="OHN27_002292"
     CDS             17501..18115
                     /gene="evgA"
                     /locus_tag="OHN27_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCQ2286723.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            18120..21713
                     /gene="evgS"
                     /locus_tag="OHN27_002293"
     CDS             18120..21713
                     /gene="evgS"
                     /locus_tag="OHN27_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCQ2286724.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPEHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(21769..22914)
                     /gene="yfdE"
                     /locus_tag="OHN27_002294"
     CDS             complement(21769..22914)
                     /gene="yfdE"
                     /locus_tag="OHN27_002294"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCQ2286725.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGTHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(22988..23932)
                     /gene="yfdV"
                     /locus_tag="OHN27_002295"
     CDS             complement(22988..23932)
                     /gene="yfdV"
                     /locus_tag="OHN27_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCQ2286726.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLFVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(24002..25696)
                     /gene="oxc"
                     /locus_tag="OHN27_002296"
     CDS             complement(24002..25696)
                     /gene="oxc"
                     /locus_tag="OHN27_002296"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCQ2286727.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDMHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(25750..27000)
                     /gene="frc"
                     /locus_tag="OHN27_002297"
     CDS             complement(25750..27000)
                     /gene="frc"
                     /locus_tag="OHN27_002297"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCQ2286728.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(27513..28148)
                     /locus_tag="OHN27_002298"
     CDS             complement(27513..28148)
                     /locus_tag="OHN27_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCQ2286729.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            28444..28719
                     /gene="ypdI"
                     /locus_tag="OHN27_002299"
     CDS             28444..28719
                     /gene="ypdI"
                     /locus_tag="OHN27_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCQ2286730.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGGSERSYKETEKTSAM
                     TTTHSTKLQLSQAILFKMREDAPPLNLTEEMPPPFPTKANYLIHPVR"
     gene            complement(28796..29038)
                     /locus_tag="OHN27_002300"
     CDS             complement(28796..29038)
                     /locus_tag="OHN27_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCQ2286731.1"
                     /translation="MINLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            29391..30311
                     /gene="lpxP"
                     /locus_tag="OHN27_002301"
     CDS             29391..30311
                     /gene="lpxP"
                     /locus_tag="OHN27_002301"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCQ2286732.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            30667..30738
                     /gene="ypdK"
                     /locus_tag="OHN27_002302"
     CDS             30667..30738
                     /gene="ypdK"
                     /locus_tag="OHN27_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCQ2286733.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(30803..32041)
                     /gene="alaC"
                     /locus_tag="OHN27_002303"
     CDS             complement(30803..32041)
                     /gene="alaC"
                     /locus_tag="OHN27_002303"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCQ2286734.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADALLPASSKHIHENAE"
BASE COUNT         8881 a         7643 c         7126 g         8544 t
ORIGIN      
        1 agaatttaat caagtttcaa ttacatcctg cctgattatt caactctatt ttctctatta
       61 tctgtattaa taaatgacaa tgagtgtcaa acagacatta ttcatttcaa catgtaactt
      121 tgggcaaaat caagatacgc gcagaagaat aatttccgaa tgaaagtaga aagtgtttta
      181 tttatagatg gggagagtct tcgatagtag tgaggttgac agatatccgc tgcctgatga
      241 gcaattatgc ccaatgaatc tacctcattg ggcataattt ggaactcatc agatagcttt
      301 tgccatcttc agattacacg ggctcatctg ccagttgaat gtgccgtttc cgctttcatc
      361 aagcgttacg ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc ccggacataa
      421 ctcggcaacc agatacccga ctaacggtaa gtgagagatc accagtaccg aagcaacacc
      481 ttcattggtc aacgcctgca aataggcact gaccagacca acatcgccgc agggcgttaa
      541 ctccggcaga acttctgcac tggaaggcag gttcagacaa tcccctactt cttccagtgt
      601 ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg atttccactt tttgaccttt
      661 cagccagttc gccatcaggc gagattcgtc acaaccatta gtggtcagag gacgaaccga
      721 atcactggcg gcatcgaggg ctgcgtcgcc gtgacgcatg ataaaaactt gcatattgca
      781 ccgcttttgt taaccagatt cgcccatttt ctttcatctg aaaccagaga aagaaaacgg
      841 tggccggcat tgtgccttat ccattcaccg aatgaaacgc tgttttttac ctcaatggcg
      901 taagtatagt caatatgcgt ttacattttg accaacactc cgccattcag cgcggattca
      961 tatagctttg accttcttat tgcaggtcag ttgcagttgt tttccaaaaa ctttccccac
     1021 gcgcgcccat ctcgaccaaa cgctcgcaag gggtaaaacg ggaaccatac tgcgtggcca
     1081 gtcgttgcat tattgcaacc acttcgcccg cgccgagaga atcgatatag cggaacggtc
     1141 caccgagaaa tggcggaaaa ccaatgccaa ataccgcacc aatatccccg tcacgcacgc
     1201 tacggataac ctgctcatca acacaacgta ctgcttcatt cagcatcaac atcacacacc
     1261 gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg agcgggtaaa
     1321 tggcaggatc gacctgtttt ttgcttttac gccctttctg accataaaga tagaaacccc
     1381 gaccattttt tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca tttgcaggcg
     1441 cgctaaaacg ttctccataa gcggcttcca gtacaggaat aattttagtc ccggtgtcga
     1501 ttcctacctc atccaaaagt tggattgggc ctaccggaaa accaaatttc actagcgcag
     1561 catcaatgtg ctctacccgt tcaccttcgg tcagcatgcg gatagcttca ttaatgtaag
     1621 gcgctaagat gcgattgacg taaaacccgg ctttgtcacg cacgacaatg ggagttttgc
     1681 cctgtttttt cgccagtttt accgtagtgg cgatggtttg cgccgatgta cccgcatgag
     1741 gaataatctc taccagcggc attttttcca ccggactgaa gaaatgcagg ccaataactt
     1801 gctcaggtcg cgcggcgtga gcggcgatat caccaatcgg taaagatgac gtgttcgaag
     1861 caaaaatggt atgagcggcg caattttgct ccacttccgc caccatctgt tgttttaatt
     1921 cgagattttc aaacaccgct tcaataatca gatcgcgatg ggcaaagccg cgatagtccg
     1981 tcgttccgga gattaatgcc agctgtttgt cacgttcgct ggctttgaga tgacgacggc
     2041 gaactttgcc ctccagctga tcccaactgt acttcagcgc atgatttatg ccctgcgggt
     2101 tgatatcttt aattctgacc ggaagccccg ctttacaagc ggtgacataa gcaataccgg
     2161 cgcccatcaa gccaccacct aaaatcccca cgctgtttaa tggcgcaggc ggcgcatcac
     2221 tgccgggatc tttcttcacg tccgtactgg caaaaaagat actacgcaga gcctgcgatt
     2281 gcggcgtcat cgccagttcg ccaaacgccc gtgcttcagc gtcatagcca ctgctggtgc
     2341 cctgcgctaa tcccgtttcg acaacctcca ggatgcgttc tgtcgccgga tagttacctt
     2401 gagttttgtg ttctgttttc ttgccgacca ttttgaacag cagcgcacga cctaacggcc
     2461 ccgccagaat acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc ttctttgcca
     2521 gctcaacagc ggcttccagc agaatggagt gcggaacgac gtcatccacc agccccagct
     2581 ttaatgcctg tttcgcccga agttgttttc cggtgaggat catctctaat gctgtgctga
     2641 cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat ccaagttgta
     2701 cttcaggcaa accgagcacc gttttaggat cgtcagtaca aacgcgaccg tggcacgcca
     2761 gcgccaactc cagcccacca cccaggcaag cgccatgaat agccgcgata accggaatgg
     2821 gcaaagcatg aatctccgcc atcaactgtt ggccctgccg cgccagagct tccgcttctt
     2881 gcgccgtttt gcagttgccg atcatgttga tgtctgctcc ggcgataaaa ttgtccggtt
     2941 tcgcggagat aaacaccacg ccgcgcaact ctttgttttc acggagttgc ttaataatgg
     3001 cgcgcacctg cgaggcaaat tccgccttca gggtattcat tttctcatcc ggtacgtcga
     3061 tggtgatgat agcaatgttg tccagacgaa cattaagagt aaacgctgat gccatttcca
     3121 ttattccgcc tccagaacca ttgccgcgcc aagcccaccg gcagcacagg cggtaactaa
     3181 accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca tccgtgcgcc
     3241 ggtcgccgcg aagggatgtc cgtaagcaat cgaaccgcca agcacgttaa atttgctatc
     3301 gtccacttcg ccagtggcat gtgcacgccc cagtacatca cgagcaaaac gttcactacc
     3361 cagcaactga atattcgcca gtgtctgagc tgcaaaggct tcgtgcatat cgatcaatgt
     3421 cagatcgctc atcgtcaaac cggcacgctc cagcgccagc ggtgttgacc aggctggacc
     3481 gagcaacatg tcctgccaga catcaatcgc agtaaatgcg tagctgcgca gataccccag
     3541 cggcaccagc cctaattctt tcgcccggga ttcagtcatc aggatcaccg ctgccgcgcc
     3601 atcggtcagc ggcgtactgt ttgccgccgt taccgttccg tgtttgcgat caaacgccgg
     3661 gcgcagcttt gcgtaatcgg caagcgagga attaccgcga atattgttgt cttcgacaag
     3721 cggttgttta taaggaggga taaaggcagt catcacctct tctttgagtt ttccgtctga
     3781 ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct gttctcgggt
     3841 gatgccgtag gttttcgcca tttgctctgc ggtgtcaccc atccgcaagc cggtagaata
     3901 ttctgctacc gcaggcggta cgggcattaa gtcgcgcaaa cgcaggcgag agaagagttt
     3961 caggcgctgt ctcatgattc gagctttgtt gaaatcaacc agcacgcgcg ccagtttttt
     4021 actgacgcca attggcaata cggaagagga atctgcccca ccggcaatcc ccgctcgaat
     4081 agttcccgcc atcaggcttt ctgcgacgtt tgcaactgcc tggaaactgg tagcgcaagc
     4141 gcggctgacg ctgtaagcat cggtatgcac attcattccc gtaccgagaa caatttcacg
     4201 cgcaatgttg ggggcttcag gcatttgtac gacctgacca aagaccagtt gttcaatcac
     4261 ttcagcgggg atctcgctgc gtgccagcag ttcgcctacc accatcttcc ctaaatcaac
     4321 cgcgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta aaccgctaac
     4381 aatggcgata cgatcgccct ggcgggtaac cagcggtaaa acctgcccca taacactccc
     4441 ctgtaaaaaa taaataaagt ggtctgacct gatcatagtc ttaaccattt ttttacattt
     4501 agccaagcgg agaaaaggga aagtgggagc tatgacacag agagaaaaga agaagagaaa
     4561 agaaaacgcc cctgccagca actgacaggg gcgtacgtac ccgagaggaa ttaacgcaga
     4621 cccagctgga agatcagcat ttcagcttcg caggcaaaag tgaaatcgat atccaggcgc
     4681 acaccgtcag actcttcagt gaaagtcggg gagattttgc acggttcgga ttccacgcta
     4741 cgggctttct cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt tgcaaacacg
     4801 cggctgtaag acgcggtgca gtcggagttg tccataatgg tgccaacatc catacagcag
     4861 caaaccgggg tttcatcagc actacattta ctcatcgttg atttcctctg tatgtgcacc
     4921 caaggtgcca gataaacgtt gtggatattt tacgcttccg gaaagtgctg ctccagttgt
     4981 taattctgca aaatcggata agtgaccgaa atcacactta aaaatgatct aaaacaaaat
     5041 tcacccgaat ccatgagtgc gccacctcca aattttgcca gctggatcgc gtttcttaga
     5101 tcatatttga aaaaagatag aaacatactt gcaacattcc agctggtccg acctatactc
     5161 tcgccactgg tctgatttct aagatgtacc tcagacccta cacttcgcgc tcctgttaca
     5221 gcacgtaaca tagtttgtat aaaaataaat cattgaggtt atggtcatga gccagaaaac
     5281 cctgtttaca aagtctgctc tcgcagtcgc agtggcactt atctccaccc aggcctggtc
     5341 ggcaggcttt cagttaaacg aattttcttc ctctggcctg ggccgggctt attcagggga
     5401 aggtgcaatt gccgatgatg caggtaacgt tagccgtaac cccgcattga ttaccatgtt
     5461 tgaccgcccg acattttctg cgggtgcggt ttatattgac ccggatgtaa atatcagcgg
     5521 aacgtctcca tctggtcgta gcctgaaagc cgataacatc gcgcctacgg catgggttcc
     5581 gaacatgcac tttgttgcac cgattaacga ccaatttggt tggggcgctt ctattacctc
     5641 taactatggc ctggcaacag agtttaacga tacttatgca ggcggctctg tcgggggtac
     5701 aaccgacctt gaaaccatga acctgaactt aagcggtgcg tatcgcttaa ataatgcatg
     5761 gagctttggt cttggtttca acgccgtcta cgctcgcgcg aaaattgaac gtttcgcagg
     5821 cgatctgggg cagttggttg ctggtcagat tatgcaatct cctgccgggc aaactcctca
     5881 agggcaagca ttggcagcta ccgccaacgg tatcgacagt aataccaaaa tcgctcatct
     5941 gaacggtaac cagtggggct ttggctggaa cgccggaatc ctgtatgaac tggataaaaa
     6001 taaccgctat gcactgacct accgttctga agtgaaaatt gacttcaaag gtaactacag
     6061 cagcgatctt aatcgtgcgt ttaataacta cggtttgcca attcctaccg cgacaggtgg
     6121 cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag tgtcaggtta
     6181 taaccgtgtt gatccacagt gggcgattca ctatagcctg gcttacacca gctggagtca
     6241 gttccagcag ctgaaagcga cctcaaccag tggcgacacg ctgttccaga aacatgaagg
     6301 ctttaaagat gcttaccgca tcgcgttggg taccacttat tactacgatg ataactggac
     6361 cttccgtacc ggtatcgcct ttgatgacag cccagttccg gctcagaatc gttctatctc
     6421 cattccggac caggaccgtt tctggctgag tgcaggtacg acttacgcat ttaataaaga
     6481 tgcttcagtc gacgttggtg tttcttatat gcacggtcag agcgtgaaaa ttaacgaagg
     6541 cccataccag ttcgagtctg aaggtaaagc ctggctgttc ggtactaact ttaactacgc
     6601 gttctgataa cacgttcgcc tggataaagt cacctgcata gcaggtgact ttaactcccc
     6661 cacttcaccg aaagtagtgt cctcattgct tacctcctga gttttgcaaa cagcctgacg
     6721 tcagcttacc ctttcaaatc aatcagtggt atccacagac aaacctgaag gggaacgcat
     6781 tttttcaggc gttctgctcg tccttctcaa agagtttact tttctgaatt tccaggatac
     6841 tcaccccccc tggatattgt gcgctcatac acgcaaatta aagataggtt ctaaataaat
     6901 gagcgttttt tgatagtcta ttttattagg taatatatat ttgtaacaaa tcaatcaaca
     6961 tggaataaaa tcatgctacc atctatttca atcaacaata ccagcgctgc ttacacagaa
     7021 tccatcaatg aaaataacaa tgatgaagtt aatggattag tacaagagtt caaaaacctt
     7081 tttaatggta aggaaggaat aagcacctgt attaaacatc tacttgagct tataaaaaat
     7141 gccatacgag gaaacagcaa tcctgagaga attaatatca ataattccgc agttacttat
     7201 atcgatattg gctccaatga tacagaccat attactatta gtatcgataa ccaagaacca
     7261 ataaaattac ctgcgaacta taaagacaaa gaactcgtcc gtactatcat taatgacaac
     7321 atagttgaga agactcatga tatcaataac aaggaaatga tcttcagcgc attaaaagaa
     7381 atatatgatg gagatcctgg ttttatcttc gataagatat cacacaaact cagacatacg
     7441 gtaacggaat ttgatgagag cgggaaaagc gaaccaacgg acttatttac ctggtacggt
     7501 aaagataaaa aaggcgactc tctcgctatt gtaattaaaa ataaaaacgg aaatgattac
     7561 ttatctctcg gttactacga tcaggacgac taccacattc aaagaggaat tcgtattaat
     7621 ggtgatagtc tcacccaata ttgtagtgaa aacgccagga gtgcttcagc gtggtttgaa
     7681 agcagtaaag ctatcatggc agaatcattt gcaactggtt ccgatcatca ggttgtaaac
     7741 gagctcaacg gggaaagact gagagaacca aacgacgttt ttaaacgtta tggtcgagca
     7801 ataagatatg attttcaagt ggacgatgca aaatataaat gcgaccatct aaaagaaata
     7861 gtttctactt tagtcggtaa caaaattaac gttggccatt ctcaaaaaat atataagcat
     7921 tttaaggatc tcgaaggtaa aattgaagaa aggcttcaaa atcgccaggc tgaatatcaa
     7981 aatgaaatta atcaaccatc tgcgccaggt gttaattttg atgatattta aaattatctt
     8041 tccagttttt tgatttttca acagctgaat atcggaaata gtcagtgacg aacatcagta
     8101 tttttacata attacgtcta ggtcattgtc ggtttatctc cttttacttg tggtaattga
     8161 gggataacaa caaaaaaggt gagttttgcg actcaccttt tttatttgtt tcttattcag
     8221 aatcaatatc ttttaaatca tcctgaatcg cttgtgcgtt cggattttcc tgcggtttga
     8281 gttcgccgcc attagcgatg aaatcatgac gctggaagta cgcttcgcgc accataatat
     8341 aaggatcgga cgactgacgc agcagaccat cggaatccag cagctgagcg cgggtttcga
     8401 tcccttcaag cgtccattta cccacagaca tcggccaggt cagccaggaa agaaccgggt
     8461 aaagaccatc cgccatatca ccaccgtcat cacgcagcgt gaagctaccg tagaacggta
     8521 actgaacgta aggcccataa cccacgccat aatgaccaag cgtactaccg aagcggtgag
     8581 gttcagtccg ttgcagtttc gggttcgcca tccctgcaac atcaataaag ccgcccatcc
     8641 ccaaaatggt gttcaggaaa aagcgggtaa agtggaccat cccctgataa gggtcgccct
     8701 gcaagaagta gttaaccatc accgcaggtt cttcaaggtt gccagtaaag ttgctcaaac
     8761 cattacgcgc cggttgcgga acataatcac gccaggcgac agcgaccggt cgaacaatat
     8821 acgggtctaa tacgttgaag ttgaagttgt acatggtgcg gttgaaccct tctaacgggt
     8881 cagaacgccc ttgctgatct gtaccggaac tcgcacaccc caccagaagc gtagttccca
     8941 gagcaagcgc cgacaggcga agcttcataa atgtctccct gtttttttat ggcttatgca
     9001 gtttgccatc catgacggaa cgataccgta tccgcctgtt taggtgtggg cgattgtaac
     9061 agcacgtcaa ctgatgtcca gacgccctga tttgctgatt tgatcatagc ctggtaatcg
     9121 ccgccctgta ggctacttga ttctatagaa acagaaaaag gcaaacgcag ccttttctac
     9181 attttcagag taactcccgt cagttgcgag caaaaaagcc gctacgcttt agctatacgt
     9241 gctaattcaa gagaagagac catggacaac gacaaaattg atcaacacag cgacgaaatt
     9301 gaagttgaga gcgaagaaaa agagcgcggc aaaaaaatag aaatagatga agaccgactc
     9361 ccctcccggg cgatggcaat tcatgagcat atccgccagg atggtgaaaa agagctggaa
     9421 cgcgacgcaa tggcgctact gtggtcagcc attgcggcgg gtctgtcgat gggcgcttcg
     9481 ttactggcaa aagggatatt tcatgtcgaa ctggaaggtg tgccgggcag cttcttgctg
     9541 gagaatctcg gttatacctt tggttttatt atcgtcatta tggcccgcca gcaattattt
     9601 accgaaaata ccgtgactgc ggtactaccc gtcatgcaaa aaccgacaat gagcaacgtc
     9661 ggcttactta tacggttatg gggcgtcgtg ctgctgggta atattctcgg gacaggtatt
     9721 gcggcgtggg catttgaata tatgcctatc ttcaatgaag aaactcgcga tgcatttgtc
     9781 aaaatcggca tggatgtgat gaagaacacc cccagcgaga tgtttgccaa cgcgatcatt
     9841 tccggctggc tgatcgccac tatggtttgg atgtttcctg cagcgggtgc ggcaaagatt
     9901 gtggtgatta tattgatgac ctggcttatt gccctgggtg acaccaccca tatcgtggtc
     9961 ggttctgttg aaatcctcta tctggtgttt aacggtacgc tgcactggag cgatttcatc
    10021 tggcccttcg cactacctac tttagcgggg aacatctgcg gcggcacctt tatcttcgcg
    10081 ttaatgagtc atgcacagat tcgtaacgac atgagcaata agcgtaaagc agaagcacgc
    10141 caaaaagcag aacgtgcgga aaacattaag aaaaattata aaaacccggc ataaatggcg
    10201 agggtttaag caatcgagcg gcagcgtact taccccgcac tccattagcg ggtatactca
    10261 tgccgcattg tcctcttagt taaatggata taacgagccc ctcctaaggg ctaattgcag
    10321 gttcgattcc tgcaggggac accatccttg tgtttatccc taaaaccaca taaaaaccgt
    10381 aaattaaatt cgaactagca ggccgaataa ggcacaccag gaactcgtgg ttttattcat
    10441 tatgaagtgg tttttacctg ttctcttagc caggtaataa acgcttcaat ttttggccac
    10501 tgcctgcccg gtaatgtggt gatgtaataa tgctgatggc atttcaccgt catatcgcca
    10561 aacggcgcga cgagctcacc actggcaagc cttttttgta ccaggcgttt tcttcccatc
    10621 gccaccccaa tatgattcat cgcggcaata acagctaaat cagaacgatc aaagccaatt
    10681 ccagaagatg tcggcaaatt aaccgcataa tgttgcgccc aactatgcca ttcatccgta
    10741 ccggagtcgt tgctccatgc ctgtctgtca tggagcaacg tacagtgaca caggttaatt
    10801 accgtgttgg ttaaagcatg tctttgagcg tattccgggc tacatactgg caggatttct
    10861 tcatccatca gaaagtgatg agtgagttgc gctgacggcg catcatcaaa gtagatcgcc
    10921 aaatcgattc cggcacgttg cagattgacg ttgtcattac cagtgagcac ggtgagcgaa
    10981 atagacggat accggcgtgt aaagtcacct aatgcgggca ccaaccagca ttgggcgata
    11041 gagggccggg aatacagagt taacgttccc gataactcct gatttttgat atcaagaatt
    11101 tcctggttca gggtatccag cgacgatttt agcgcccaat aaacacgttt cccctcgtgc
    11161 gttaattcca ctttgcgatg ggaacgaaca aacaactgaa tgcccaattc ttcttccagc
    11221 tgattgatac ggtgacttac cgcactgggg ctcagcgaca attcctctgc cgccagggcg
    11281 aaggactgat gcctggcagc cacttcaaaa gtatgcattt ttgataattg ccagccgtta
    11341 agcagccgat ttcttatttc acgaaggggt tccataatca cctcattttt cgcttaagtg
    11401 taaaaaaata gcggcaaaat ttcagctatg agatgagcta aagtgaacca tatctcaatt
    11461 caccttcatt tttagatgta aatcactcca ttgatgcaat ttacctcatg tgaaaggcaa
    11521 attttatcgt ttgtcagcct gcgttgtttt tttgtccaat atcatcaggt taatcacagg
    11581 ggaaggtgag atatgcactc tcaaatctgg gttgtgagca cgctgcttat cagcatcgtg
    11641 ttaattgtac tgaccatcgt gaagttcaaa ttccacccgt ttctggcgct gttgctggcc
    11701 agcttcttcg tgggaacgat gatgggcatg gggccactgg atatggtaaa tgctattgaa
    11761 agtggaattg gcggaacgct ggggttcctc gcagcggtta tcggccttgg cacgatactg
    11821 ggaaaaatga tggaagtatc cggggccgca gaaagaattg gtctgacact tcaacgctgc
    11881 cgctggcttt cagttgatgt cattatggtg ctggttggcc tgatttgtgg catcacgctg
    11941 tttgttgaag tgggcgtcgt gctattgatt cctctggctt tttcaattgc caaaaaaacc
    12001 aatacctcat tattaaagct tgccattccg ctatgtaccg cattgatggc agtgcactgc
    12061 gtggttcctc cacatccggc tgctttatat gttgccaata agctgggcgc agatatcggt
    12121 tcggtgatcg tctacggttt gctggttggg ctgatggcat cactgatcgg tggcccactt
    12181 ttccttaaat ttctgggtca acgactgccc tttaaacctg tacccacaga gtttgcagat
    12241 ctcaaagttc gcgatgaaaa aacactaccg tcattaggcg caacgttatt caccatactg
    12301 ctacccattg cgctgatgtt ggttaaaacg attgccgaat tgaatatggc gcgtgagagt
    12361 ggtttgtata tcttggttga gtttattggc aaccctatca ctgccatgtt tatcgccgtg
    12421 tttgtcgcct attatgtgtt gggtatacgc cagcatatga gcatggggac gatgctcaca
    12481 catacggaaa atggcttcgg ttctattgct aatattttgc tgattatcgg ggccggaggc
    12541 gcattcaacg ccattttaaa aagcagcagt ctcgctgata cgctggcagt tattctctcc
    12601 aatatgcata tgcacccgat tcttctggcc tggttagtgg ctcttattct gcatgcggca
    12661 gtgggctccg ctaccgtggc aatgatgggg gcaacggcaa ttgttgcacc catgctgccg
    12721 ctttatcccg acatcagccc ggaaattatt gcgattgcta tcggttcagg tgcaattggc
    12781 tgcacgatcg ttacggactc gcttttctgg ctagtgaagc aatattgcgg cgctacgctc
    12841 aatgaaacat ttaaatacta tacgacagcg acatttatcg cttcagtcgt cgctctggcg
    12901 ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag acgtactatg gaaaacgcta
    12961 aaatgaactc gctcatcgcc cagtatccgt tggtaaagga tctggttgct cttaaagaaa
    13021 ccacctggtt taatcctggc acgacctcat tggctgaagg tttaccttat gttggcctga
    13081 ccgaacagga tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc tatctggcaa
    13141 aagcatttcc tgaaactgct gccactgggg ggattattga atcagaactg gttgccattc
    13201 cagctatgca aaaacggctg gaaaaagaat atcagcaacc gatcagcggg caactgttac
    13261 tgaaaaaaga tagccatttg cccatttccg gctccataaa agcacgcggc gggatttatg
    13321 aagtcctggc acacgcagaa aaactggctc tggaagcggg gttgctgacg cttgatgatg
    13381 actacagcaa actgctttct ccggagttta aacagttctt tagccaatac agcattgctg
    13441 tgggctcaac cggaaatctg gggttatcaa tcggcattat gagcgcccgc attggcttta
    13501 aggtgacagt tcatatgtct gctgatgccc gggcatggaa aaaagcgaaa ctgcgcagcc
    13561 atggcgttac ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag gaaggacgta
    13621 aagcagcgca gtctgacccg aactgtttct ttattgatga cgaaaattcc cgcacgttgt
    13681 tccttgggta ttccgtcgct ggccagcgtc ttaaagcgca atttgcccag caaggccgta
    13741 tcgtcgatgc tgataaccct ctgtttgtct atctgccgtg tggtgttggc ggtggtcctg
    13801 gtggcgtcgc attcgggctt aaactggcgt ttggcgatca tgttcactgc ttttttgccg
    13861 aaccaacgca ctccccttgt atgttgttag gcgtccatac aggattacac gatcagattt
    13921 ctgttcagga tattggtatc gacaacctta ccgcagcgga tggccttgca gttggtcgcg
    13981 catcaggctt tgtcgggcgg gcaatggagc gtctgctgga tggcttctat acccttagcg
    14041 atcaaaccat gtatgacatg cttggctggc tggctcagga agaaggtatt cgtcttgaac
    14101 cttcagcact ggcgggtatg gccggacctc agcgcgtgtg tgcatcagta agttaccaac
    14161 agatgcacgg tttcagcgca gaacaattgc gtaatgccac tcatctggtg tgggcgacgg
    14221 gaggtggaat ggtgccggaa gaagagatga atcaatatct ggcaaaaggc cgttaataac
    14281 gtttcaacgc agcatcgcaa tcctttccct gggtgagcga tgctgccgat ggcgcagact
    14341 taagatcccc ggtcttaccc gctataaccc cctcctttta tcatcaccca acgcctttcg
    14401 ctgtaaacgg cggtttcgca aaccaaacca aaaccgtaag taagataaaa gcaatagccg
    14461 ccatacggaa aatctcattt gcagaaatag aaagtgactg ctgggttatt tcattattaa
    14521 tttcattaag aactcctgaa agcgaaccat agtatttatc cataatttgt gacgatgaat
    14581 taaacacggg gttaaattga tcgatggttg ctgtcaactg actatggtgt aacgattcgc
    14641 gtcgtcccca cagcgtcatt gtcaacgacg taccaactga tcctgacaag gtacgaaaaa
    14701 aattactcat actcgaggca ttggcaaatt tattatctgg caagcctgaa aacgaaatcg
    14761 ttgttaaggg taaaaagaaa caggcaacgg cgaatccctg aaaaaactgc ggcataatga
    14821 tacctgtaaa atcaatcgtt ggcataaatg tcacagaacg ccagtaatag caaaccgcat
    14881 acatcaaaaa actgaatgtc accaacaccc gcatgtctat tttgttgcca taacgtccta
    14941 tcaaaggtga aattaatagt ggcatgatgc cgatgggcgc ataagcaagt ccggcccata
    15001 tcgcattata ccccatcgtt tcctggagta actgcggcat aaggacgatc gctccagagt
    15061 aaaataaata cgcgcatgtg atactcacaa taccaatggt gaagttacgg gacttaaaca
    15121 aactgagatc aagaatcggg ttctctgagg tcgactccca aatgactaaa gagatcagag
    15181 aaataactga tactactgtt aatattatta tcgtactcga gttgaaccaa tccagatcgc
    15241 gccctttatc aagcataatt tgcaagccac caacaccgag cactaacagg gtcagtcctg
    15301 gtagattcat tttgaccggt gaagtctcag tttctcttcc tttaagtaag gttaagcata
    15361 atgtcaggac gacaatcccc atagggacat tgattaaaaa tatccaaccc cagctaaagt
    15421 tatcacaaat atagccgccc aatatcggcc cacatatcgg agcgataatc acggtcattg
    15481 accataatgc cagagcaaat gtcctttttt ctggtgggta attccttaat aataaactct
    15541 gtgacagtgg aattaacggc cccgccatta acccctgaac gactctaaaa aatatcagca
    15601 catcaagatt ggtcgatagg ctacacatta atgaagacag cgaaaaaaag gttactgaaa
    15661 gtaaaaataa tcttaattcg cctattcttt gtgccaacct gccagtaaca gggatcgcaa
    15721 tggcatttgc tacaccaaac gaggtgataa cccaggtgcc ttcgtctgtt gatgctccca
    15781 gaaagccaga tattgtcggt attgcgacgt tagaaatagt ggaatccaac atttgcataa
    15841 atgtcgctaa tgacaatgca atagtgacgc accataacgt cccaccggtt aatggtgccg
    15901 gagttgattt agtgattgcc attaaagttg tccattatgc gaaataatgt tgctaatttc
    15961 tttttctatc ggactggtat cgataactaa agccttactg gtataagccg gcatggaggt
    16021 cacggttgaa gccagctcag gcatctcggc aatgtcttcg ttcttcgtat caatagttgc
    16081 tgtcatcgat aaaccaatac gcaagggatg ttccatgagt tcttttggat caagagaaac
    16141 ttcaaccggt acacgctgaa cgattttgat ccagttccct gtcgcatttt gtgcaggtaa
    16201 taaggagaac gcattgccgg ttcccatatt gatccctgtc acccgaccat gaaacacaac
    16261 attttcacca taaagatcgc tgataatatt gaccgattga ccaatccgta catccgtgag
    16321 ttgtgtttct ttaaagttgg cattaaccca catttgacgt gccggtacga cagccattaa
    16381 cgattgtccg gggctcactg tttcgccgac ctgaacactt ctctgggcaa tatagccggt
    16441 aaccggactc ttaatatccg tacgtttaag cgccaaccag gcttctttag ttgcatccgc
    16501 cgcttcaacg acttgtggct gacggtttaa tggtgtgttc attactaaag ctttattcgc
    16561 tttataagcc tggatagcgg cattcaatgc cgctttgcta cttattaacg tatctttggt
    16621 atgctccagc gtttcttttg aaataacccc ctgcttcgct aacggcactc gacggttata
    16681 atcttctaaa gattgttgat actgaatacg tgctgaagcg acttcggcac tgtattgttt
    16741 atcctgtaag tatagtttat tcgtttgccg aacaatattt gccagattat ttttagcttt
    16801 attgagtgcg atagtggcat cagttttatc cagtgaaact aaaatgtcac cttgtcgaac
    16861 gtagttcgta tctttatgat taacgacagt gacactacct gagacttgtg cagaaattgg
    16921 atctgcattc cccgtgacat aggcgtcatc tgtactaatc atgtcttcta attccattga
    16981 ccaataggca taggcacctg aaaacgcaat aaataaaact accgccaata aagaaaagta
    17041 ttttcttctg ttagaatgtt ttttatttga attaatctgt tccactatta tctctcattt
    17101 ctcatagatg aaatttatga attgaatgaa aagagcattc attcttcata tcaaacacag
    17161 tgcatgtgtt gcacattatc ccaggaacgt tggaggccta atttaaatga ggtgcaaaaa
    17221 aaacaacaga ggactaaacc gtggcttttg caatacaatt cttacgcctg taggattagt
    17281 aagaagactt atagtgccaa cttgaaacta taaatcatcg gtacaatccc tgattttatt
    17341 gttgacattt catttatgcc gactatttat atggtatact tgtcgaatta tcttaaagga
    17401 agctcagatt ttcttatttt tattgagaaa atgagatgac gccttatgtc tgtattacta
    17461 cagggagaag ggagatgctt cattgcaaag ggaataatct atgaacgcaa taattattga
    17521 tgaccatcct cttgctatcg cagcaattcg taatttattg atcaaaaacg atattgaaat
    17581 cttagcagag ttgactgaag gcggaagtgc cgttcagcgg gtggaaacac ttaagcctga
    17641 tatcgtcatc attgatgtcg atatccccgg agttaacggt atccaggtgt tagaaacgct
    17701 gaggaagcgc caatatagcg gaattattat tatcgtctcc gctaaaaatg accattttta
    17761 cgggaaacat tgtgctgatg ctggcgctaa tggtttcgtg agtaaaaaag aaggcatgaa
    17821 caatatcatt gcggctattg aagctgcaaa aaatggctac tgctatttcc ccttctctct
    17881 caaccggttt gttggaagtt taacgtccga ccagcaaaaa ctcgactcct tatcgaaaca
    17941 agaaattagt gtcatgcggt atattcttga tggcaaggat aataatgaca ttgctgaaaa
    18001 aatgttcatc agcaacaaaa ctgtcagcac ttataaaagt cgcctgatgg aaaaattaga
    18061 atgtaaatca ctgatggatc tttacacatt cgcacaacgt aacaaaatcg gctaaccaca
    18121 tgaagttttt accctatatt tttcttctct gttgtggtct ttggtcgacc ataagtttcg
    18181 cagacgaaga ttacatcgaa tatcgtggca tcagtagtaa caaccgtgtc acacttgatc
    18241 cactacgtct gagcaacaag gaattacgtt ggttagcgag caaaaaaaat cttgtgattg
    18301 cagtacataa gtcccaaacg gctacgttgt tgcataccga ttcgcagcaa cgggttcgtg
    18361 gtattaatgc tgattattta aatcttttaa aaagagcgtt aaatatcaaa ttaacactcc
    18421 gggaatacgc agatcatcaa aaagcaatgg acgcgcttgc agaaggtgaa gtcgatatag
    18481 tgttatcaca tttagttact tcgccgcctc ttaataatga cattgctgca accaaaccat
    18541 tgataattac ctttccggcg ctggtaacca cccttcacga ctcaatgcga ccgcttacct
    18601 caccaaaacc agtaaatatt gctcgggtag caaattaccc cccagacgag gtaattcatc
    18661 aatcatttcc aaaagcaaca attatctctt ttacaaattt atatcaggca ttagcatccg
    18721 tctcagctgg gcacaatgat tactttattg gtagtaacat cattaccagc agtatgattt
    18781 cccgctattt cactcactcc ttaaatgtag tgaaatatta taactcgccg cgtcaatata
    18841 attttttctt gaccagaaaa gaatctgtca ttcttaatga agtactcaat agatttgttg
    18901 atgctttaac aaatgaagtt cgctatgaag tatcacaaaa ttggcttgat acaggaaacc
    18961 tggcctttct gaacaaacca ttagaactca ctgaacatga aaaacagtgg attaagcagc
    19021 atcccaattt aaaggtgctg gaaaatcctt actcgccccc ctattctatg acggatgaaa
    19081 atggctcggt tcggggcgtt atgggggaca ttcttaatat tattaccttg caaacaggtt
    19141 taaatttttc tccgatcacc gtttcacaca atatccatgc tggaacacag cttagccccg
    19201 gaggatggga tataatacct ggcgctattt atagtgaaga tcgagaaaat aatgttttat
    19261 ttgctgaagc cttcataaca acgccttacg tttttgtcat gcaaaaagcg cctgacagtg
    19321 aacaaacatt aaaaaaagga atgaaagttg ccattccata ttattatgag ctgcattcgc
    19381 aattaaaaga gatgtatccg gaggttgaat ggatacaggt cgataatgcc agcgctgcat
    19441 ttcacaaggt taaggaaggt gaacttgatg ctctggtcgc gacacagcta aattcgcgtt
    19501 acatgatcga tcattactat cctaatgaac tttatcattt tcttattcct ggcgttccga
    19561 atgcatcgct ttcgttcgct tttcctcgcg gagaaccgga acttaaggat attattaata
    19621 aagcactgaa tgcaattccc ccaagcgaag ttctgcgcct gacggaaaaa tggattaaaa
    19681 tgcccaatgt gaccattgac acatgggacc tatatagcga gcaattttat attgttacga
    19741 cattatccgt tttattagtt ggcagtagcc ttttatgggg attctacctg ttacgctcag
    19801 ttcgtcgtcg taaagtcatt cagggtgatt tagaaaacca aatatcattc cgaaaagcac
    19861 tctcggattc cttaccgaat ccaacttatg ttgtaaactg gcaaggtaat gtcattagtc
    19921 ataatagtgc ttttgaacat tatttcactg cggattacta caaaaatgca atgttaccat
    19981 tagaaaacag tgactcaccc tttaaagatg ttttttctaa tgcgcatgaa gtcacagcag
    20041 aaacgaaaga aaatcgaaca atatacacac aggtatttga aattgataat ggcatcgaga
    20101 aaagatgcat taatcactgg catacattat gcaatcttcc tgcaagtgac aatgcagtat
    20161 atatttgtgg ttggcaagat attactgaaa cgcgtgatct aattaatgca ctcgaggtag
    20221 aaaaaaataa agcgataaag gctaccgtag caaaaagtca gtttctggca acgatgagtc
    20281 acgaaataag aacaccaata agctctatta tgggcttcct ggaacttctg tcgggttctg
    20341 gtcttagcaa ggagcaacgg gtggaggcga tttcacttgc ctacgccacc ggacaatcac
    20401 tcctcggctt aattggtgaa atccttgatg tcgacaaaat tgaatcgggt aactatcaac
    20461 ttcaaccaca atgggtcgat atccctactt tagtccagaa cacttgtcac tctttcggtg
    20521 cgattgctgc aagcaaatcg atcgcattaa gttgcagcag tacgtttcct gaacattacc
    20581 tggttaagat cgaccctcag gcgtttaagc aggtcttatc aaatttactg agtaatgctc
    20641 tcaaatttac caccgagggg gcagtaaaaa ttacgacctc cctgggtcac attgatgaca
    20701 accacgctgt tatcaaaatg acgattatgg attctggaag tggattatcg caggaagaac
    20761 aacaacaact gtttaaacgc tacagccaaa caagtgcagg tcgtcagcaa acaggttctg
    20821 gtttaggctt aatgatctgc aaagaattaa ttaaaaatat gcagggcgat ttgtcattag
    20881 aaagtcatcc aggcatagga acaacattta cgatcacaat cccggtagaa attagccagc
    20941 aagtggcgac tgtcgaggca aaagcagaac aacccatcac actacctgaa aagttgagca
    21001 tattaatcgc ggatgatcat ccgaccaaca ggctattact caaacgccag ctaaatctat
    21061 taggatatga tgttgatgaa gccactgatg gtgtgcaagc gctacacaaa gtcagtatgc
    21121 aacattatga tctgcttatt actgacgtta atatgccgaa tatggatggt tttgagttga
    21181 ctcgcaaact ccgtgagcaa aattcttcct tacccatctg ggggcttaca gccaacgcac
    21241 aggctaacga acgtgaaaaa gggttaagtt gcggcatgaa cttatgtttg ttcaaaccgt
    21301 tgaccctgga tgtactgaaa acacatttaa gtcagttaca ccaagttgcg catattgcac
    21361 ctcagtatcg ccaccttgat atcgaagccc tgaaaaataa tacggcgaac gatctacaac
    21421 tgatgcagga gattctcatg actttccagc atgaaacgca taaagatcta cccgctgcgt
    21481 ttcaagcact agaagctggc gataacagaa ctttccatca gtgtattcat cgcatccacg
    21541 gtgcggctaa catcctgaat ttgcaaaagt tgattaatat tagccatcag ttagaaataa
    21601 cacctgtttc agatgacagt aagcctgaaa ttcttcagtt gctgaactct gtaaaagaac
    21661 acattgcaga gctggaccag gagattgctg ttttctgtca gaaaaatgac taaatagcgg
    21721 ctcccacaat gttcaaatgt gggagctatt taccagcaca tcttacattt atgatgagaa
    21781 ctcctggcga atttgttccc catgctggtc gagcgttgcc gctcccggca taacatgcgg
    21841 gtccgcgcag ccgctgattt ttatcggatt acccggcatc attattcccc cggcttcaat
    21901 caacatattt ctcgcctgag tttgtggcaa tttaatggcc tcagccacac ttaataacgg
    21961 cgcgacgggt acaccaactt catgtattct ggctaaccaa acttcagctg cctgcgtttt
    22021 taacgtccgc tcaatatatt gtttaagaat agcctggttt tgtacgcgta aaatattgct
    22081 gctaaatcgg ggatcattaa ccagttccgt aagctccagt gcctggcata acgcagaaaa
    22141 aagcttgtca ttaccacaac aaatcgtaat cggcttatcc tgagtattga aaacatcaaa
    22201 aggtgccatg tagggatggc gatttcccag acgttgtggt gacttccctg tcgcgatata
    22261 tgccatcaga ccatgctcca gaaaactcag cgtggcatca aacatcgcta tatcgacatg
    22321 cgtccctctc tggctctttt cacggccata aagtgcactc actattccgc tgaataaata
    22381 gacaccgccg catagatccg caagagatgt accaacgcgc actggcggag catcaggata
    22441 tcctgtttcc atcattatcc cgctcattgc ctgaatgatg gtatcgtagg caggagcatc
    22501 ttttagcgga ccggtatgtc cgaaacctga cgatgaagca tatatgaggc gcgggttgat
    22561 ttcttgaagc gtttcccatg aaaaccccag tttttccatt gtacctgggc gaaaattctc
    22621 agctaataca tcagcttgtt tgagcatatt tataaatata cttttatcgt gatcattctt
    22681 taaatcaaga accacactct ctttgccatg attaataaaa ctgtaataga gtgactgtcc
    22741 atccacatag ggaccaaatg tgcgggtatc atcaccatga cccggcggct caactttaat
    22801 tacccttgcg cccatattac aaagaagttg agttccgaaa ggtccattaa ggacatgtgt
    22861 catatcgata actaataagc cttcaaacgg gcctttgctt tcattatttg tcatttgctt
    22921 atgccttgaa aaaagccgaa ggtgaaaaga cacttcggca tttatgtaaa atcaataaca
    22981 tgattaatta tgaaaccagt cgactgacat aaatccacaa gggagccgtg acgacaaagc
    23041 ccagtacgct cacagccaat gacgctgtac cagtacgggt gtagacatta aaccggctgg
    23101 caataatgat tccggagaat gccggcggta atgcgcctgc cagtaccatc atctgcagat
    23161 gttcgctgtt caaatgacat gccataccaa cgaaaagcag tgccagtggc atcagaatca
    23221 gcttcaggaa ggtgttataa gcaatttcgg cactgaactc gaatttatgt gcagccagag
    23281 tcaaccccgc agcgaatacc gctacccctg agttagcctt cgcaatcaga ttaaaggttg
    23341 ggtcccatgc tgccggaatt tttaccccaa ccaacaccag gatcgttgcc agaacaggtg
    23401 cccatactac tggctccttt gccgcagaaa ttaatgcgct cagattacta ttcttcttac
    23461 catccgctcc tgaagaagga ttcagcaaat acagaccaat aggaatagta attgcgttaa
    23521 caataataga aataattgct accactaaac ctgttgatac ggaatcacca taaataggat
    23581 cgagaactgc aaaccccagg aatccaatgg taggtgaacc tgcaattaat gcacatacag
    23641 ctgcttctgc atgggtacgt ttaaaaaatt tgtagcaacc gaaccaggag aaaaagaaac
    23701 atccgacaat aaccacaagt gatacaaggg tcagacgagt gtccgcaaaa atcatttccc
    23761 tgtttgcccg agtaatagat acaaatagag ccgcaggaag cgcgtagttt aataccagtt
    23821 tattaaatgc ccgagcttga tcttctgaaa atgtttctcg tctgccgcta aaataaccca
    23881 acagcatgat tacgataata ggcaataaat cgccaataaa aaatgttagc ataatagtat
    23941 ttccgataag ggtcccctca ccatcgcata atgagttggc aagaggagac gatgtcagta
    24001 tttaattacc agcgacttgt tttgggttaa gtttggtaat atggccactt tcagttcctg
    24061 ctgcagggtc gatgaccaca ttaataatgg tcggtttgcg cgactgaata ccggtggtta
    24121 aagcatgacg aagttcatct gtcgtggtga cgttatagcc aacgccacga aacgcatcca
    24181 ttaatttgtc ataccttgca tggtgcaaca gatccgttgg tgatggtgca ccagcgccac
    24241 tgagatcaac accgtctcct ctgtagatgc cgccattatt aaaaataacg atcgtcaccg
    24301 gcaggttata tcgacaaatc gtttcaattt ccatcccact gaaaccaaaa gcactatcac
    24361 cttcaatggc gacaaccgga gaaccagaag tcacgctagc accgatggca tagcccatac
    24421 cgatgcccat gacaccccag gtaccacaat ccagacgacg acgtggttta tacatatcaa
    24481 taatatttcg tgcattatcc agggtatttg caccttcatt aactaaataa atatcctggt
    24541 tctcgcgcaa tacgtcgcgc acagcactta atgcattaaa gtaatttaat ggttgagtat
    24601 ctgtacttaa tttttcatgc attttttgtg cattttgctg cttgtggata tttaaaatat
    24661 cgcgccatac cagtggagtc gtaaatgtgt tttgtttcag ttctgccagc ataccttgca
    24721 tactggatgc aatatcacca acgactggca cagcaatggg gcggttgctg tcaatttcct
    24781 gcggttcaat atccagttga ataaactgtg tatctgccgc ccatcctttt ttaccgtgtg
    24841 ccaataacca attcagtcgt gcaccaacaa gcatgacaac gtcagcattt gccagggcaa
    24901 acgaacgcgc agctgccgca gaaagtggat gcatatcttc aaggatccct ttcgccatag
    24961 acattggcag gaatggaatc tgagcacttt caataaattc acgaagctgt tcatcagctt
    25021 gtgaatacgc cgcgcctttg ccaaggataa ttaatggccg ttcagctttt gctaaaagcg
    25081 aaattgcgct agtgactgac ttcgggcatg gcaataatgc tggcgacgga ttttcaactt
    25141 taacaatcgt ggttaacgct tcgtcttttt ccatcgtcgc ggccaggaca tttgctggca
    25201 aatcaagata aactccgcca gggcgacccg atacagagac ccggatagcg cgtgccaatg
    25261 caatgccaag atcctgcggc tgattaacgc gaaatgctgc tttggcatac ggttttgccg
    25321 cattcatttg gtccagctct tcataatcac cttgctgtag gtcgacgatc gcgcggtcgc
    25381 tggagccgct aatcatgatc atcggaaaac catttaccgt tgcgttggcc aatgcggtca
    25441 aaccattgag gaatcctggc gcagaaactg tcaggcagat ccccggtttt tgggtaagaa
    25501 aaccgcttgc cgcagcggca tagcctgccg actgctcatg acgaaaacca atataacgaa
    25561 tgccttccgc ctgggcatgg cgtgccatat ccgtcacagg aatacctaca acaccataaa
    25621 tagtgtcaat attattctgt tttaatgctt caacgatgat atgcatacca tctgtcattt
    25681 gaagttgatc tgacatgatt aataacctcg aaatttttat aaattgggag ccagaagccc
    25741 ccgttaatat cagatggcgt ggttttgctt cattgcagca atttcatcgt cgctataacc
    25801 cagctcctgc aatacagcag cggtatgttc acctaatagc ggcgcagctt taatatccgg
    25861 cgtaaaggca gagaatttca ttggacaacc aacggtcaga tattttccac gcaacggttg
    25921 ttccacttca acaacactgc cactttggcg caaagaggga tcaagtgaaa tttctttcat
    25981 acttaaaacc ggtgcacaag gaatatcaaa ctgagtcaaa taggccaccg cttcatgttt
    26041 atcaatagtg acagtgtatt tttcgatttc agcaaaaata tcgaaaatat gtggctgtcg
    26101 tgcatgggct gtactgtatg ccggatcggt aatccattct ggtttgccga tggctttaca
    26161 ggtgttttcc cagttttgct cctgaatagt gaaataaata taggcgttag gatcggtttc
    26221 ccagccttta catttcagga tccagccagg ctgaccgcca ccacctgcat taccaccgcg
    26281 gggaactgca tcaccaaatg taccattcgg atactgcggg tattcttcca gataacccaa
    26341 tttatcgaga cgctgctggt cacgtaattt cacgcggcaa aggttcaata cggcatcctg
    26401 cattgacatg gtgactcgtt gcccacgccc cgttttttcg cgatgcagca aagcagcaag
    26461 taaaccgatc agcaaatgca ttccggtgtt gctgtcaccc aacgctgcag cgcttaccag
    26521 cggcggacca tcccaaaaac ccgtagtgga tgccgcgcca cccgctgcct gagcaacgtt
    26581 ttcataggct tttacattca cataaggcga acactcatca aaccctttga tcgaaccaaa
    26641 aatcagacgt ggattgattt cttgaatatg ctcccaggtg aagcccatgt gatcaatggc
    26701 ccctggatga aagttctcga ctaagatatc agcttcgcgg atcagctttt ccattacctc
    26761 tttgccttcc gctgttttgg tatttaactc aatagaacgt ttgttactgt taagcatggt
    26821 gaagtaaagc gcatcgatat caggaatatc tcgcagctgg tgacgcgtta cgtcaccaac
    26881 gccgggacgt tcaattttaa taacgtcagc gccaaaccag gccagcattt gagtacaaga
    26941 tgggccagat tgcacaccgg tgaaatcgag aactttaatt ccttgaagtg gagttgacat
    27001 agcaatacct attagttaat taaatatgtg ttaatttagc gtttatttaa ggcgtaatat
    27061 cttctataaa taacccgcaa aatatcgtcg tagctgcact cgatattttt aaaatcctgt
    27121 atcaagtgct gaaaatcatt tcatcgccac ttccaattcc tttctgcatg gtaagtagtg
    27181 aggacgaaaa gaagaatatg ataaatattg attcgtggta atcagagaga gtcgcgacat
    27241 ttgtgatgtt attcttacaa ctgtaagaag cgccggtgat ttacaagcaa gggtatgatt
    27301 acgcttttta aagcgtacca taaaacataa cataataaat gatttgtgat gacacgggat
    27361 tattgcggtt tactttgagg aatataaaat gcgagtttat agcaccgcga gcgccaaata
    27421 attttatgac cacaacctgt cgtattgtgg tcataaaaaa ttgtcggctg gagggagtta
    27481 aatcctttac ctcaatatac tgcctgtcag cattagttca caaacagtgc ttcgctatcg
    27541 acgatgattc catcttccgc acctttaagc gccaggtttg cctcgtagta ttgcttttta
    27601 tcaagcagtt tctgcgcatc agcaagtgca ttacgagtct gtttaagtgg catcagatat
    27661 tggttttcca ttacgccaac accggccagg cgaagctctt ccattgcgcc ctttttatcc
    27721 cctttcgcca ttttttcatt ggcaattttt atcgcggctt ctttttcggg agttgctaca
    27781 taactctcag aaattccgac agatgcatta atgacaatat attggtcatc attgagattg
    27841 gtctttttac ctggcttagc gaactttgcc cattcggtac tgtcatctga cagcaaagcg
    27901 gaagcttcat tggtcagttc ttttgccttt tctggatcgc catgaaacag agccaggcgc
    27961 gctacctgca cgtcacgcat cgcatataaa ccttgttcag agatacgctc tgctgccgct
    28021 gccgttttct gggtttgctg aacttgctga gtttgctgtt gtgccgctac cggagcatta
    28081 tcagcagccc atacagttga agatgccaga attgctgtga ccatcgtggc cataattaaa
    28141 cgtttcatac ttacctcatt ctttaacagt tttagaaaac gccgccatca tattgggtga
    28201 gcatgaaatg agaaatcaga gagcaacggt cattttgaaa ggattgtctt acagttgtag
    28261 ttattatcgc aaagcggcat ttagtcttac agttgtagga atatgcttcc tgtcgcagag
    28321 aactattctt atataccaca aagagatgtc atttattata tcgatgtaaa tactgattaa
    28381 ttaaatgtaa atacagccat aacatcgtag gttctcagtg aaccattttc aggataactt
    28441 tctatgaaag taaacttaat acttttcagc ttatttttat tggtctctat tatggcatgc
    28501 aatgtttttg cattttccat ttcgggtggt ggaagtgaga ggagctataa agagactgaa
    28561 aaaacatcag cgatgacgac cacacactct acaaaacttc agctatcaca ggcgattttg
    28621 tttaagatga gagaagatgc gccaccatta aacctcacag aagaaatgcc gccccctttt
    28681 ccgacaaagg cgaattatct tattcatcct gtgcgatagc gtctttcaca tggcaggtga
    28741 acaatcatgt ctgatgctga tgtgaacacc tgcccaaatg agccatcctg acccactacc
    28801 gcctgccata ccccaccata aagcgaatca tcaagccaca agccggggca agaaacataa
    28861 atatccccgt cactagttca ttaccgccag ttaaccacat ggtgaagtaa taaatgagtg
    28921 cagcgaggat caccatgccg aagaaagaag agaccgccga taccacgttc agtacctgac
    28981 cgatgtagcc gcttacgcag acaatacaca gggcgagaaa catccataaa ttaatcatag
    29041 ctcaacctcc tgccgcgcta aaaaacctgt aacttcagtc actttaatag taagacaatc
    29101 cttaacctct ggcaattttt gctaagcatc aatgaaaaca gcatgttaaa tgcaagactg
    29161 ttgtgtacgg aaaaatattt actttgcacg attaataatc agtagctgaa agcagtcagc
    29221 gagtgataaa gtacagatac cgcattcatg ctcctcgtgg ttatgtcctg actagtcttt
    29281 acactcttta caggaaccat tgtcgtacat gatggcccaa ccaattgagt gtaccgctgt
    29341 cgacatggaa tcttctgcga tacaacaatt cgtatctaca gaaggtaact atgtttccac
    29401 aatgcaaatt ttcccgcgag tttctacatc ctcgctactg gctcacatgg tttgggcttg
    29461 gtgtactctg gctttgggta cagcttcctt atcctgttct ctgctttctc ggcacgcgta
    29521 ttggcgcaat ggcgcgacca ttcctgaaac gtcgtgaatc tatcgcccgt aaaaacctgg
    29581 aactttgttt cccgcagcat tctgcggaag aacgcgagaa gatgattgcc gaaaactttc
    29641 gttcactcgg catggcgctg gtagaaaccg gcatggcatg gttctggccc gacagtcgcg
    29701 tacgtaaatg gtttgatgtt gaagggttgg ataaccttaa acgcgcacaa atgcaaaatc
    29761 gcggcgtaat ggttgtcggc gtccatttta tgtcgctgga actgggcggc cgcgtgatgg
    29821 gactgtgcca accaatgatg gctacctatc gtccacataa taatcagctg atggaatggg
    29881 tgcagacccg tgggcgcatg cgctctaaca aagcgatgat cggcagaaat aatctgcgcg
    29941 gcattgtcgg tgcactgaag aaaggtgaag cggtatggtt tgctcccgat caggattatg
    30001 gtcgtaaagg cagctccttc gcgccgttct ttgcggtgga aaatgtcgcc acaaccaatg
    30061 gcacctatgt tctctcccgt ctctctggcg cagccatgtt gaccgtaacg atggtaagaa
    30121 aagcggatta cagcggatat cgtttgttca tcaccccaga gatggaaggc tacccgacag
    30181 atgaaaatca agccgctgcc tatatgaaca agattatcga gaaagagatc atgcgcgcac
    30241 cggagcagta cctctggatc caccgtcgct ttaaaacgcg cccggtggga gaatcgtcgt
    30301 tgtacattta acaaactgct cttactcttt ccctcctcaa atcggccata ataacctcat
    30361 ggtttttatg gcttttttct taaggttttt tccttcgctg aatatctaat tattaccgtc
    30421 agaaattaaa ctgtcgccga tatgagacac ttgtaagtta ctaaaagtaa ataagaaaat
    30481 gcctcttgtc tcgtttcagt tttaggcgta cattagcgcc gtctcgagca gtttagctca
    30541 gaattctggg gtgatgccca cggtaaaata cagggctttt aaggtcgctt tgcccggtgt
    30601 caactcactg tatcaggtgt aatgaagtca ttcaggcgta acagtaatta cgcggagaga
    30661 tgtaaagtga aatatttctt tatgggcatt tcttttatgg tcatcgtttg ggccggtact
    30721 tttgccctga tgatctaaaa gcaaactgtg caatgaaaaa caggagccat caggctcctg
    30781 tttgcatttc tgacgacggt gtttattccg cgttttcgtg aatatgtttg ctgctggcgg
    30841 gtagtaaagc gtcggcacgg aacatcgctt taatccctct gatggcctga cgaatacggt
    30901 cgcggttttc aatcagtgca aagcgaacat gcgtatcacc atagtcgcca aagccaatcc
    30961 ccggcgagac acaaaccttc gcttcgttaa ggagcttctt ggcaaattcc agcgatccca
    31021 tggccgcata tggttccggg attttcgccc agacatacat cgaagccttc ggcatttcaa
    31081 ccatccagcc cgcttcatgc agccctttaa ccaatacatc acggcggcgt ttgtactgtt
    31141 cggcaatgtc gcgcacgcac tgttgatcgc cctccagcgc cgcaatcgcc gccacctgca
    31201 acggcgtaaa ggtgccgtaa tcgtgatagc ttttaatacg agccagggcg ctgaccagcg
    31261 ttttgttgcc aaccataaag ccgatacgcc agcccgccat gttgtagctt ttcgacagcg
    31321 taaagaactc gactgccaca tcgcgtgcac ccggtacctg cataattgac ggcgctttcc
    31381 agccatcgta gacgatatcg gcataggcca ggtcatggac caccagcaca tcgtagcgtt
    31441 tcgccagcgc cactactttt tcgaagaact ccagttccac gcattgcgcg gttgggtttg
    31501 acgggaagcc gaggatcatc atcttcggtt tcggataact ttcacgaatg gctcgctcca
    31561 gttcgttgaa gaaatcgaca ccttccacca gcggcactga gcgtacctgc gccccggcaa
    31621 tcaccgcgcc ataaatatgg atcgggtaac ttggattcgg caccagcacc gtgtcaccat
    31681 gatccagcgt cgccagcatc agatgcgcca ggccctcttt cgaaccaata gtgacgatgg
    31741 cttctgattc cgggtcgatt tcaacgtcgt agcgatcctg ataccagcgg gaaatggcgc
    31801 gacgtaaccg cggaatgccg cgtgaagtgg agtaaccatg cgtgtccggg cgctgggcca
    31861 cagtacataa tttttcgacg atatgcggcg gagtcgcacc gtccgggtta cccatgctga
    31921 aatcgataat atcttcgccg cgccgacgcg cagccatttt cagttcagcg gtgatgttaa
    31981 aaacgtaggg cgggagacga tcaatgcgcg taaagcgacg ttcagggcga gtgtcagcca
    32041 taatttcctc agattaacgt tagcgcccgg accgtccgag cgacgctgcc acaatggtgg
    32101 ctgttttgaa aatagcctga ttaatttctg tctgtccagc gttttagaac atttttatca
    32161 atggctttaa atgaggaact caacttcaaa aaca
//
LOCUS       DAJOSY010000049        31834 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJOSY010000049 DAJOSY010000000
VERSION     DAJOSY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.049"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(57..947)
                     /gene="rarD"
                     /locus_tag="OHN27_002304"
     CDS             complement(57..947)
                     /gene="rarD"
                     /locus_tag="OHN27_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCQ2286735.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(999..1466)
                     /locus_tag="OHN27_002305"
     CDS             complement(999..1466)
                     /locus_tag="OHN27_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCQ2286736.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1631..2500
                     /gene="pldA"
                     /locus_tag="OHN27_002306"
     CDS             1631..2500
                     /gene="pldA"
                     /locus_tag="OHN27_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCQ2286737.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2633..4462
                     /gene="recQ"
                     /locus_tag="OHN27_002307"
     CDS             2633..4462
                     /gene="recQ"
                     /locus_tag="OHN27_002307"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCQ2286738.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4526..5146
                     /gene="rhtC"
                     /locus_tag="OHN27_002308"
     CDS             4526..5146
                     /gene="rhtC"
                     /locus_tag="OHN27_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCQ2286739.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5208..5828)
                     /gene="rhtB"
                     /locus_tag="OHN27_002309"
     CDS             complement(5208..5828)
                     /gene="rhtB"
                     /locus_tag="OHN27_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCQ2286740.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAVHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5939..6961
                     /gene="pldB"
                     /locus_tag="OHN27_002310"
     CDS             5939..6961
                     /gene="pldB"
                     /locus_tag="OHN27_002310"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCQ2286741.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6969..7769
                     /gene="yigL"
                     /locus_tag="OHN27_002311"
     CDS             6969..7769
                     /gene="yigL"
                     /locus_tag="OHN27_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCQ2286742.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            7845..8744
                     /gene="bioP"
                     /locus_tag="OHN27_002312"
     CDS             7845..8744
                     /gene="bioP"
                     /locus_tag="OHN27_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCQ2286743.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8632..9585)
                     /gene="metR"
                     /locus_tag="OHN27_002313"
     CDS             complement(8632..9585)
                     /gene="metR"
                     /locus_tag="OHN27_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCQ2286744.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9822..12083
                     /gene="metE"
                     /locus_tag="OHN27_002314"
     CDS             9822..12083
                     /gene="metE"
                     /locus_tag="OHN27_002314"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCQ2286745.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSVPIQARRHSTRVHNPAV
                     EKRLATITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12123..12938)
                     /locus_tag="OHN27_002315"
     CDS             complement(12123..12938)
                     /locus_tag="OHN27_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCQ2286746.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAKDGWQRMLEWFKQYGGKKSL"
     gene            13200..13961
                     /gene="udp"
                     /locus_tag="OHN27_002316"
     CDS             13200..13961
                     /gene="udp"
                     /locus_tag="OHN27_002316"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCQ2286747.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14102..15529
                     /gene="rmuC"
                     /locus_tag="OHN27_002317"
     CDS             14102..15529
                     /gene="rmuC"
                     /locus_tag="OHN27_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCQ2286748.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15624..16379
                     /gene="ubiE"
                     /locus_tag="OHN27_002318"
     CDS             15624..16379
                     /gene="ubiE"
                     /locus_tag="OHN27_002318"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCQ2286749.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16393..16998
                     /gene="ubiJ"
                     /locus_tag="OHN27_002319"
     CDS             16393..16998
                     /gene="ubiJ"
                     /locus_tag="OHN27_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCQ2286750.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16995..18635
                     /gene="ubiB"
                     /locus_tag="OHN27_002320"
     CDS             16995..18635
                     /gene="ubiB"
                     /locus_tag="OHN27_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCQ2286751.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18714..18983
                     /gene="tatA"
                     /locus_tag="OHN27_002321"
     CDS             18714..18983
                     /gene="tatA"
                     /locus_tag="OHN27_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCQ2286752.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18987..19502
                     /gene="tatB"
                     /locus_tag="OHN27_002322"
     CDS             18987..19502
                     /gene="tatB"
                     /locus_tag="OHN27_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCQ2286753.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19505..20281
                     /gene="tatC"
                     /locus_tag="OHN27_002323"
     CDS             19505..20281
                     /gene="tatC"
                     /locus_tag="OHN27_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCQ2286754.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20323..21105
                     /gene="tatD"
                     /locus_tag="OHN27_002324"
     CDS             20323..21105
                     /gene="tatD"
                     /locus_tag="OHN27_002324"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCQ2286755.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21102..21590)
                     /gene="rfaH"
                     /locus_tag="OHN27_002325"
     CDS             complement(21102..21590)
                     /gene="rfaH"
                     /locus_tag="OHN27_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCQ2286756.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21757..23250
                     /gene="ubiD"
                     /locus_tag="OHN27_002326"
     CDS             21757..23250
                     /gene="ubiD"
                     /locus_tag="OHN27_002326"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCQ2286757.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALRKVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23296..23997
                     /gene="fre"
                     /locus_tag="OHN27_002327"
     CDS             23296..23997
                     /gene="fre"
                     /locus_tag="OHN27_002327"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCQ2286758.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24280..25443)
                     /gene="fadA"
                     /locus_tag="OHN27_002328"
     CDS             complement(24280..25443)
                     /gene="fadA"
                     /locus_tag="OHN27_002328"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCQ2286759.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGMVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25453..27642)
                     /gene="fadB"
                     /locus_tag="OHN27_002329"
     CDS             complement(25453..27642)
                     /gene="fadB"
                     /locus_tag="OHN27_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCQ2286760.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27832..29163
                     /gene="pepQ"
                     /locus_tag="OHN27_002330"
     CDS             27832..29163
                     /gene="pepQ"
                     /locus_tag="OHN27_002330"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCQ2286761.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29163..29777
                     /locus_tag="OHN27_002331"
     CDS             29163..29777
                     /locus_tag="OHN27_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCQ2286762.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29816..31267
                     /gene="trkH"
                     /locus_tag="OHN27_002332"
     CDS             29816..31267
                     /gene="trkH"
                     /locus_tag="OHN27_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCQ2286763.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31279..31824
                     /gene="hemG"
                     /locus_tag="OHN27_002333"
     CDS             31279..31824
                     /gene="hemG"
                     /locus_tag="OHN27_002333"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCQ2286764.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7358 a         8131 c         8740 g         7605 t
ORIGIN      
        1 caatatgccc actctcatgc aagagtcggc atattttttc agaatatatt tattttttat
       61 ttggacgttc tacgctgcgt ataaatcgca tccatcacaa aaattgccag cgccacccaa
      121 ataaaggcga aagtcaccat cttatcggca cccggttttt caccataaaa cgtcacagcc
      181 agcaggaaca tcagcgtcgg gccaatgtac tggaaaaagc ctaacgttga gagacgcaag
      241 cgcgtggcag cggcggtaaa acacaacagc ggtacggtag tgacaatacc ggcggcgatc
      301 agcagtaaat tcagcgacat cgggttttgc cccatatggc tggttgagct gtcggcaata
      361 gcaaacaggt aaattgccgc cacgggcagc agccacatgg tttcgattaa catgccggtt
      421 tgcgcttcaa cggcaatctt cttgcgtacc agaccgtaga aggcaaaact aaatgccagt
      481 cccagcgcga taataggtag cgaaccaaaa gtccacagct ggactaacac gccacatatc
      541 gccagaatca ccgccagcca ttgcatccgg cggaatcgct cgccgaggaa aatcatcccc
      601 agcacaatgt tcaccagcgg gttaataaag taaccaaggc tcgcttccag catatggtga
      661 ttgttcaccg cccagataaa cagtagccag ttgccaccaa tcagcacggc agagactgcc
      721 agcataaaaa ttttctgtgg cgtctgaatc agcgttttta aataggacca ctggcggcaa
      781 atgctcatca gcaccaccat aaagaaaaac gaccagatca cgcgatgcgt caggatttca
      841 tcggcgggca cgtagtaaat caacttgaag tacgctggcg ctatacccca aataaaataa
      901 gcggcaagag cgagtaatac gccctgccgc gtttgttttg catccatcgg gaatactcat
      961 ttttaattgg taacagcagt ttacctgctt ttatgtcttc aacctaccat ataggtggcg
     1021 gtggcactgg caatataaag ctgttcttca ttgtgtaatt caacgcgggc gacggcgact
     1081 ttattgcctg cacgcaacag gctactagta gcagtaaaac gctcgcccct gcctgggcgc
     1141 agataatcaa cgcgaagatc aatggtcccc atccgcgata gccgctggcg tagttcatct
     1201 tcactgatgg tttcgtggcg ggttaaggta cttcccacgc acaccagacc ggcggcgaca
     1261 tccagcgccg acgcaatgac cccgccgtgc aaaatgcttt gcgcccagtt gcccaccatc
     1321 attggctgat ttttaaaggc cagctgtgcg aactcttttt cgtaacgctc cagttccatc
     1381 cccaatgcgc ggttaaatgg catgtgataa acaaacatct cacccactaa tttcagggct
     1441 tgttcagcgg tcagtacggc agacatatca tccttacact tcattggtta atgaaatgtt
     1501 gattttatgc ttctttgttg ttggtttcta ctttaggaag ggataactaa cggctatgga
     1561 gttaagtatg taaaatagcc cgcagaaaaa tattcacctt atcaataatt cgttacggag
     1621 aacacgaccg atgcggactc tgcagggctg gttgttgccg gtgtttatgt tgcctatggc
     1681 agtatatgca caagaggcaa cggtgaaaga ggtgcatgac gcgccagcgg tgcgtggcag
     1741 tattatcgcc aatatgctgc aggagcatga caatccgttc acgctctatc cttatgacac
     1801 caactacctc atttacaccc aaaccagcga tctgaataaa gaagcgattg ccagttacga
     1861 ctgggcggaa aatgcgcgta aggatgaagt aaagtttcag ttgagcctgg catttccgct
     1921 gtggcgtggg attttaggcc cgaactcggt gttgggtgcg tcttatacgc aaaaatcctg
     1981 gtggcaactg tccaatagcg aagagtcttc accgtttcgt gaaaccaact acgaaccgca
     2041 attgttcctc ggttttgcca ccgattaccg ttttgcaggt tggacgctgc gcgatgtgga
     2101 gatggggtat aaccacgact ctaacgggcg ttccgacccg acctcccgca gctggaaccg
     2161 cctttatact cgcctgatgg cagaaaacgg taactggctg gtagaagtga agccgtggta
     2221 tgtggtgggt aatactgacg ataacccgga tatcaccaaa tatatgggtt actaccagct
     2281 taaaatcggc tatcacctcg gtgatgcggt gctcagtgcg aaaggacagt acaactggaa
     2341 caccggctac ggcggcgcgg agttaggctt aagttacccg atcaccaaac atgtgcgcct
     2401 ttatactcag gtttacagcg gctatggcga atcgctcatc gactataact tcaaccagac
     2461 ccgtgtcggt gtgggggtta tgctaaacga tttgttttga tgaacggttg agtggttggc
     2521 aaatctggaa cccagcatcc aggattaccc tctcagagac taaaagcatt gcagtttctc
     2581 gcgcaggcgc tgaaaatagc gcctgttttt atttcaggca atcggggtga atgtggcgca
     2641 ggcggaagtg ttgaatctgg agtccggagc taaacaggtt ttacaagaaa cctttggcta
     2701 ccaacagttt cgccccggcc aggaagaaat tatcgacact gtgctttccg gtcgcgattg
     2761 cctggtcgtc atgcccaccg gtggcggaaa atccctttgc tatcaaattc ctgccttatt
     2821 gctaaacggc cttaccgtgg ttgtttcacc gctgatttcg ttgatgaaag atcaggtgga
     2881 tcaactgcaa gccaacggcg tggcggcggc gtgccttaac tcgacgcaaa cccgcgaaca
     2941 gcaacttgaa gtgatgacag gctgccgcac cgggcaaatt cgcttactgt atatcgcccc
     3001 ggaacgcctg atgctggata actttcttga gcatctggcg cactggaatc cggtgttatt
     3061 agccgtcgat gaagcgcact gtatctccca atggggccac gatttccgcc cggaatatgc
     3121 cgcgctcggt cagttgcgcc agcggttccc gacgctgccg tttatggcgc tgaccgccac
     3181 agccgacgac accacgcgcc aggatatcgt gcgcctgctg gggctgaacg atccgctgat
     3241 tcaaatcagc agttttgacc gtccgaatat tcgctacatg ctgatggaga agttcaaacc
     3301 gctcgatcag ttgatgcgct acgtgcagga acagcgcggt aagtcaggca ttatctactg
     3361 caacagccgc gcgaaagtag aagacaccgc tgcgcgcctg caaagcaagg gaattagcgc
     3421 ggcggcctat catgccgggc tggaaaataa tgttcgcgcc gatgtgcagg aaaaattcca
     3481 gcgcgatgac ctgcaaattg tggtggcgac ggtggcgttc ggcatgggca tcaataaacc
     3541 aaacgttcgc ttcgtggtcc actttgatat tccgcgcaat atcgaatcct attatcagga
     3601 aaccggacgc gccgggcgtg atggcctgcc cgcggaagcg atgctgtttt acgatccggc
     3661 tgatatggcg tggctgcgcc gttgtctgga agagaagccg caggggcagt tgcaggatat
     3721 cgagcgccac aaactcaatg cgatgggcgc gtttgccgaa gcgcaaactt gccgtcgtct
     3781 ggtattgctg aactattttg gcgaagggcg tcaggagccg tgcgggaact gcgatatctg
     3841 cctcgatccg ccgaaacagt acgacggttc aaccgatgct cagattgccc tttccaccat
     3901 tggtcgtgtg aatcagcggt ttgggatggg ttatgtggtg gaagtgattc gtggtgctaa
     3961 taaccagcgt atccgcgact atggtcatga caaactgaaa gtctatggca tgggccgtga
     4021 taaaagccat gaacattggg tgagcgtgat ccgccagctg attcacctcg gcctggtgac
     4081 gcaaaatatt gcccagcatt ctgccctaca actgacagag gccgcgcgcc cggtgctgcg
     4141 cggcgaatcc tctttgcaac ttgccgtgcc gcgtatcgtg gcgctcaaac cgaaagcgat
     4201 gcagaaatcg ttcggcggca actatgatcg caaactgttc gccaaattac gcaaactgcg
     4261 taaatcgatt gccgatgaaa gcaatgtccc gccgtacgtg gtgtttaacg acgcaacctt
     4321 gattgagatg gctgaacaga tgccgatcac cgccagcgaa atgctcagcg ttaacggcgt
     4381 tgggatgcgc aagctggaac gctttggtaa accgtttatg gcgcttatcc gcgcgcatgt
     4441 tgacggcgac gacgaagagt agtcagcggc ataaaaaagt gccaggatga agactccgta
     4501 aacgtttccc ccgcgagtca aatgtatgtt gatgttattt ctcaccgtcg ccatggtgca
     4561 tattgtggcg cttatgagcc ccggtcccga tttctttttt gtctctcaga ccgctgtcag
     4621 tcgttcccgt aaagaagcga tgatgggcgt gctgggcatt acctgcggtg taatggtttg
     4681 ggcagggatt gcgttgcttg gcctgcattt gattatcgaa aaaatggcct ggctgcatac
     4741 gctgattatg gttggcggtg gcctgtatct ctgctggatg ggttaccaga tgctacgcgg
     4801 tgcactgaaa aaagaggctg tatctgcgcc tgcaccacag gttgagctgg cgaaaagcgg
     4861 gcgcagtttc ctgaaaggct tactgaccaa tctcgccaat ccgaaagcga ttatctattt
     4921 tggttcggta ttctcattgt ttgtcggcga taacgttggc actaccgagc gctggggcat
     4981 tttcgcgttg attattgtcg aaacactggc gtggtttacc gtcgttgcca gcctgtttgc
     5041 cctgccgcaa atgcgccgtg gttatcaacg tctggcgaag tggattgatg gttttgccgg
     5101 ggcgttattt gccggatttg gcattcattt gattatttcg cgttgatgcc agacgcgtca
     5161 tcagagtaag tcggataagg cgtttacgcc gcatccgaca ttatttttca cgcatgcctc
     5221 gccgatgcta acagcgctcc caccagcata aacaacgagc cgaaaatctt attcagcgcc
     5281 ttcatctgct ttggtccttt aatccataga gcaatccgtt gagcaagggt ggcgtaaccg
     5341 atcatcacaa taatatcgac tacaatagtg gtgacgccga gcacgatata ctgcatcagt
     5401 tgtggctgct gtggcatgat gaattgcgga aatagcgccg ccagaaacac aatacttttg
     5461 ggattggtga gattcacaaa aactgcgcgc tggaacaaat gtcgacgcga ttgagtagag
     5521 gccagcgatt taaggtcaat tgcaccagcg gcgcgccact gctggattcc cagccaaatc
     5581 aagtaagccg cgcctgccca cttcaacact tcaaacgcaa tcactgagcg ggaaaatagc
     5641 gtccccaacc ccacgccaac cagcacaata tgaaccgcca gtccggtctg aagcccagca
     5701 atagacgcca ccgcgccgcg ataaccgtgg ttgagcgagg tggtcatagt gttgattgca
     5761 ccagagcctg gcgacaggct taaaatgatc gatgtcagca ggtaggcaaa ccaccattct
     5821 aaggtcatga tgaactcccg gtgtgtctat ttttgtgcca caatacgcta ctgtcgcagc
     5881 gttgtgtcag gcacgcttaa aaaaacgatt ttacgtggtt taagaggcag attacccgat
     5941 gtttcagcag caaaaagact gggaaacaag agaaaacgcg tttgctgctt ttaccatggg
     6001 accgctgact gatttctggc gtcagcgtga tgaagcagag tttactggtg tggatgacat
     6061 tccggtgcgc tttgtccgtt ttcgcgcaca gcaccatgac cgggtggtag tcatctgtcc
     6121 ggggcgtatt gagagctacg taaaatatgc ggaactggcc tacgacctgt tccatttggg
     6181 gtttgatgtc ttaatcatcg accatcgcgg gcagggacgt tccggtcgcc tgttagccga
     6241 tccgcatctc gggcatgtta atcgctttaa tgattatgtt gatgatctgg cggcattctg
     6301 gcaacaggag gtccagcccg gtccgtggcg taaacgctat atactggcac attcgatggg
     6361 cggagcgatc tccacattat ttctgcaacg ccatccaggt gtatgtgacg ccattgcgct
     6421 aactgcgcca atgtttggga tcgtgattcg tatgccgtca tttatggcac ggcagatcct
     6481 caactgggcc gaagcgcatc cacgtttccg tgatggctat gcaataggca ccgggcgctg
     6541 gcgcgcgttg ccgtttgcta tcaacgtact gacccacagc agacagcgct atcgacgtaa
     6601 cttacgcttc tatgctgatg acccaacgat tcgcgtcggt gggccgacct accattgggt
     6661 acgcgaaagt attctggctg gcgaacaggt gttagccggt gcgggtgatg acgccacgcc
     6721 aacgcttctc ttgcaggctg aagaggaacg cgtggtggat aaccgcatgc acgaccgttt
     6781 ttgtgaactc cgcaccgccg cgggccatcc tgtcgaagga ggacggccgt tggtaattaa
     6841 aggtgcgtac catgagatcc tttttgaaaa ggacgcaatg cgctcagtcg cgctccacgc
     6901 catcgttgat tttttcaaca ggcataactc acccagcgga aaccgctcta cagaggttta
     6961 aatttcttat gtaccaggtt gttgcgtctg atttagatgg cacgttactt tctcccgacc
     7021 atacgttatc cccttacgcc aaagaaactc tgaagctgct caccgcgcgc ggcatcaact
     7081 ttgtgtttgc gaccggtcgt caccacgttg atgtggggca aattcgcgat aatctggaga
     7141 ttaagtctta catgattacc tccaatggcg cgcgcgttca cgatctggat ggcaatctga
     7201 tttttgctca taacctggat cgcgacattg ccagcgatct gtttggcgta gtcaacgaca
     7261 atccggacat cattactaac gtttatcgcg acgacgaatg gtttatgaat cgccatcgcc
     7321 cggaagagat gcgctttttt aaagaagcgg tgttcaaata tgcgctgtat gagcctggat
     7381 tactggagcc ggaaggcgtc agcaaagtgt tcttcacctg cgattcccat gaacaactgc
     7441 tgccgctgga gcaggcgatt aacgctcgtt ggggcgatcg cgtcaacgtc agtttctcta
     7501 ccttaacctg tctggaagtg atggcgggcg gcgtttcaaa aggccatgcg ctggaagcgg
     7561 tggcgaagaa actgggctac agcctgaagg attgtattgc gtttggtgac gggatgaacg
     7621 atgccgaaat gctgtcgatg gcggggaaag gctgcattat gggcagcgcg catcaacggc
     7681 tgaaagatct gcatccggag ctggaagtga ttggtactaa cgccgaagac gcggtgccgc
     7741 attatctgcg taaactctat ttatcgtaat cgttctttat ttggtcagtt gtcaaccaga
     7801 tacttcgcta caatggatcc tcgttaatca aagagttttc cattgtggcg ctacttatca
     7861 tcaccacgat tctgtgggcc ttctccttta gcttttatgg cgagtacctt gcggggcacg
     7921 tcgatagcta ttttgcggtg ctggtgcgtg ttggcctggc ggcactcgtt tttctgccgt
     7981 ttctgcgtac ccgtggcaat agcctgaaaa cggtcggcct gtatatgctg gtgggcgcga
     8041 tgcagcttgg cgtgatgtat atgctgagtt tccgcgctta tctctacctg acggtttccg
     8101 agctgctgct gttcaccgtg ctgacgccgc tctacatcac gctgatttat gacatcatga
     8161 gtaagcgccg tctgcgctgg ggctatgcct ttagcgcctt gctggcggtg attggtgccg
     8221 ggattattcg ctatgatcag gtcaccgacc atttctggac tggcttgctg ctggtgcaac
     8281 tctccaatat cacttttgcc attggcatgg tgggttacaa acgcctgatg gaaactcgcc
     8341 cgatgccaca gcataacgcc tttgcgtggt tctatcttgg cgcgtttctg gtggcagtga
     8401 ttgcatggtt cttgctggga aatgcgcaga aaatgccgca aaccacgctg caatggggca
     8461 ttctggtgtt tcttggcgtg gtggcttccg ggattggcta ctttatgtgg aactacggcg
     8521 cgacgcaggt ggacgccgga acgctgggca ttatgaataa tatgcacgtt ccggcagggc
     8581 tgctggtaaa cctggctatc tggcaccaac agccgcactg gccaacgttt attacaggcg
     8641 cgctggtgat cctggcctca ctgtgggtgc atcgtaagtg ggtcgctccg cgctcttcac
     8701 aaacggcaga tgatcgcagg cgtgattgcg cgctgagcga ataaacgctt ccgtaactgg
     8761 ctgacgctgc tcgccatcgc gcacggcggc gtacagtcgg ctccacaagc cttcgcccag
     8821 ggttttggtc accaccagac cctggcgctc aaaactctct actacccaat gcggtagcgc
     8881 ggcaataccc atccgcgcgg caaccatctg aatcaacaat aaggtgttat cgacgctttt
     8941 cagtgacggg ctgacgcctg ccggctgaag aaaatgccgc cagacatcca gtcgactacg
     9001 ctgcaccgga taaattaata gcgtctcgct ggcgagatct tccggtgtaa ttcgcgtttt
     9061 cgccgccagt ggatggtcag gtgctaacac cagacgcact tcatagtcga acatcggcga
     9121 ataatgcagg ccactgcgcg gcagaatatc ggacgtcatt accagatcca gctctccctg
     9181 ttgcaaggcg ggctgcgggt caaacgtcac gcccgattta aaatccatct ctacctgcgg
     9241 ccagttctta tggaaatttt ctaacgcggg tgtcagccac tgaatacagc tatggcactc
     9301 aatggcaatg cgcagacgcg tctgctgcgg ttcattgcag gcttgcaggg cctggctaat
     9361 ttgcggcagt acctggtttg ccagttgcaa caggatttct ccctgcggtg taaagcgtag
     9421 cggctggctc ttacgcacaa atagccggaa gccaaggcgt tgttccagat cgctaaactg
     9481 gtgagacagg gcggattgcg tctgatgcaa cgtcgccgca gcggctgcga gcgagccgca
     9541 gttccgcaac gcttgtagcg ttttcaggtg ttttacttcg atcatgaaag tccttcactt
     9601 cggcatgaat aatttgcgct tgaggaatat acagtaaccg ccaattatgg atgtgtaaac
     9661 atctggacgg ctaaaatcct tcgtctttta aatttatggt gcgttggctg cgtttctcca
     9721 ccccggtcac ttacttcagt aagctcccgg ggatgaataa acttgccgcc ttccctaaat
     9781 tcaaaatcca taggatttac atataattag aggaagaaaa aatgacaata ttgaatcaca
     9841 ccctcggttt ccctcgcgtt ggcctgcgtc gcgagctgaa aaaagcgcaa gaaagttatt
     9901 gggcggggaa ctccacgcgt gaagaactgc tggcggtagg gcgtgaattg cgtgctcgtc
     9961 actgggatca acaaaagcaa gcgggtatcg acctgctgcc ggtgggcgat tttgcctggt
    10021 acgatcatgt actgaccacc agtctgctgc tgggtaacgt tccggcgcgt catcagaaca
    10081 aagatggttc ggtagatatc gacaccctgt tccgtattgg tcgtggacgt gcgccgactg
    10141 gcgaacctgc ggcggcagcg gaaatgacca aatggtttaa caccaactat cactacatgg
    10201 tgccggagtt cgttaaaggc caacagttca aactgacctg gacgcagctg ctggacgaag
    10261 tggacgaggc gctggcgctg ggccacaagg tgaaacctgt gctgctgggg ccggttacct
    10321 ggctgtggct ggggaaagtg aaaggtgaac aatttgaccg cctgagcctg ctgaacgaca
    10381 ttctgccggt ttatcagcaa gtgctggcag aactggcaaa acgcggcatt gagtgggtac
    10441 agatcgacga acccgcgctg gtactggaac taccgcaggc gtggctggac gcatacaaac
    10501 ccgcttacga cgcgctccag ggccaggtga aactgcttct gaccacctat tttgaaggcg
    10561 taacgccaaa cctcgacacg attactgcgc tgcctgttca ggggctgcat gtggaccttg
    10621 tacatggtaa agatgacgtt gctgaactgc acaagcgcct gccttctgac tggttgctgt
    10681 ctgcgggtct gatcaatggt cgtaacgtct ggcgcgccga tcttaccgag aaatatgcgc
    10741 aaattaagga cattgtcggc aaacgcgatt tgtgggtggc atcttcctgc tcactgctgc
    10801 acagccccat cgacctgagc gtggaaacgc gtcttgatgc ggaagtgaaa agctggtttg
    10861 ccttcgccct acaaaaatgc catgaactgg cactgctgcg cgatgcgctg aacagcggtg
    10921 acacggcagc tctggcagag tggagcgtcc cgattcaggc gcgtcgtcac tctactcgtg
    10981 tacataatcc ggcggtagaa aagcgtctgg cgacgatcac cgcacaggac agccagcgtg
    11041 cgaatgtcta tgaagtgcgt gctgaagccc agcgtgcgcg ttttaaactg ccagcgtggc
    11101 cgaccaccac gattggttcc ttcccgcaaa ccacggaaat tcgtaccctg cgtctggatt
    11161 tcaaaaaggg taatctcgac gccaacaact accgcacggg cattgcggaa catatcaggc
    11221 aggccattgt tgagcaggaa cgtttgggac tggatgtgct ggtacatggc gaggccgagc
    11281 gtaatgacat ggtggaatac tttggcgagc acctcgacgg gtttgtcttt acgcaaaacg
    11341 gttgggtaca gagctatggt tcccgctgcg tgaagccacc gattgttatt ggtgacgtta
    11401 gccgcccggc accgattacc gtggagtggg cgaagtatgc gcaatcgctg accgacaaac
    11461 cggtgaaagg gatgctgacg gggccggtga ccatactctg ctggtcgttc ccgcgtgaag
    11521 atgtcagccg tgaaaccatc gccaaacaga ttgcgctggc gctgcgtgat gaggtggccg
    11581 atctggaagc ggcagggatt ggtatcatcc agattgacga accggcgctg cgcgaaggtt
    11641 taccgctgcg tcgtagcgac tgggacgcct atctccagtg gggcgtagag gccttccgta
    11701 tcaacgccgc cgtggcgaaa gatgacacac aaatccacac tcacatgtgt tattgcgagt
    11761 tcaacgacat catggattcc attgcggcgc tggatgcaga cgtcatcacc atcgaaacct
    11821 cgcgttccga catggagttg ctggagtcgt ttgaagagtt tgattatccg aatgaaatcg
    11881 gtccaggcgt ctatgacatt cactcgccaa acgtaccgag cgtggaatgg attgaagccc
    11941 tgctgaagaa agcggcaaaa cgcattccgg cagagcgcct gtgggttaac ccggactgtg
    12001 gcctgaaaac gcgcggctgg ccagaaaccc gcgcggcact ggcgaacatg gtgcaggcgg
    12061 cgcagaattt gcgtcggggg taaaatccaa accgggtggt aataccaccc ggtcttttct
    12121 cattacagcg acttcttccc accatactgc ttaaaccatt ccagcatacg ctgccagcca
    12181 tctttcgcag atgcggcatg atagctcggg cgataatcag cattgaatgc atgcccggca
    12241 tccgggtaca cgataatctc tgctttcgca ttagcagccc gcagcgcctg gcgcatggtt
    12301 tcaacgctct cctgcggaat actgttatcc agaccgccat ataagccgag aaccggtgcg
    12361 ttaagatcgg ttgcgatatc aacaggttgt ttcggtgaat tcagcgactt gtcgcccgtc
    12421 agtttgccgt accacgccac tgcggctttt agctgtggat tatgcgcggc atacagccag
    12481 gtgatacgtc caccccagca gaatccggtg atcattaaac gatgaacatc gccgccgttg
    12541 cgggaagccc aactggcgac atgatcgaga tcggccagca cctgcgagtc aggcactttt
    12601 gctaccagac cgctaagcaa cgtggggata tcggcaaaat cattcggatc gccttcgcgg
    12661 aagtaaagtt caggtgcgat agccagatac ccctccagcg ccagacggcg acaaatgtcg
    12721 cggatatgtt catgcacgcc aaaaatttcc tgcactacaa tgaccactgg cagtgggcca
    12781 tcgctttgct ttggtctggc atggtaagca ggcatgttat ccccttgtga agggatagag
    12841 gtgaagcccg ccacaatcgc gtcgtccggg gtctgaacga tggtcgaagc gagaggcgat
    12901 gcagcaggtg caaatccaga ttgttgtgtt gttgccatgg tattctccgt acccttataa
    12961 aaatgttgcg caatgttaac tatagtcagc atgcaacaaa tcacattgcc tgaatcggct
    13021 catcttttat gcagtcctgc agaatgaaga gtgatttatg tgatttgaat cacttttggt
    13081 gggtaaattt atgcaacgca tttgcgtcat ggtgatgagt atcacgaaaa aatgttaaac
    13141 ccttcggtaa agtgtctttt tgcttcttct gactaaaccg attcacagag gagttgtata
    13201 tgtccaagtc tgatgttttt catctcggcc tcactaaaaa cgatttacaa ggggctacgc
    13261 ttgccatcgt ccctggcgac ccggatcgtg tggaaaagat cgccgcgctg atggataagc
    13321 cggttaagct ggcatctcac cgcgaattca ccacctggcg tgcagagctg gatggtaaac
    13381 ctgttatcgt ctgctctacc ggtatcggcg gcccgtctac ctctattgct gttgaagagc
    13441 tggcacagct gggcattcgc accttcctgc gtatcggtac aacgggcgct attcagccgc
    13501 atattaatgt gggtgatgtc ctggttacca cggcgtctgt ccgtctggat ggcgcgagcc
    13561 tgcacttcgc accgctggaa ttcccggctg tcgctgattt cgaatgtacg actgcgctgg
    13621 ttgaagctgc gaaatccatt ggcgcgacaa ctcacgttgg cgtgacagct tcttctgata
    13681 ccttctaccc aggtcaggaa cgttacgata cttactctgg tcgcgtagtt cgtcacttta
    13741 aaggttctat ggaagagtgg caggcgatgg gcgtaatgaa ctatgaaatg gaatctgcaa
    13801 ccctgctgac catgtgtgca agtcagggcc tgcgtgccgg tatggtagcg ggtgttatcg
    13861 ttaaccgcac ccagcaagag atcccgaatg ctgagacgat gaaacaaact gaaagccaag
    13921 cggtgaaaat cgtggtggaa gcggcgcgtc gtctgctgta attctcttct cctgtctgaa
    13981 ggccgacgcg ttcggccttt tgtatttttg cgtagcgcct cgcaggaaat gcctttccaa
    14041 ctggacgttt gtacagcaca attctatttt gtgcgggtaa gttgttgcgt caggaggcgt
    14101 tgtggatttc tcaatcatgg tttacgcagt tattgcgttg gtgggtgtgg caattggctg
    14161 gctgtttgcc agttatcaac atgcgcagca aaaagccgag caattagctg aacgtgaaga
    14221 gatggtcgcg gagttaagcg cggcaaaaca acaaattacc caaagcgagc actggcgtgc
    14281 agagtgcgag ttactcaata acgaagtgcg cagcctgcaa agtattaaca cctctctgga
    14341 ggccgatctg cgtgaagtaa ccacgcggat ggaagccgca cagcaacatg ctgacgataa
    14401 aattcgccag atgattaaca gcgagcagcg cctcagtgag cagtttgaaa acctcgccaa
    14461 ccgtattttt gagcacagca atcgccgggt tgatgagcaa aaccgtcaga gtctgaacag
    14521 cctgttgtcg ccgctacgtg aacaactgga cggtttccgc cgtcaggttc aggacagctt
    14581 cggtaaagaa gcacaagaac gccataccct gacccacgaa attcgcaatc tccagcaact
    14641 caacgcgcaa atggcccagg aagcgatcaa cctgacgcgc gcgctgaaag gcgacaataa
    14701 aacccagggc aactggggcg aggtagtatt gacgcgggtg ctggaggctt ccggtctgcg
    14761 tgaagggtat gaatatgaaa cccaggtcag catcgaaaat gacgcccgct cgcggatgca
    14821 gccggatgtc atcgtgcgcc tgccgcaggg aaaagatgtg gtgatcgacg ccaaaatgac
    14881 gctggtcgcc tatgaacgct attttaacgc cgaagacgac tacacccgcg aaagcgcgct
    14941 acaggaacat atcgcgtcgg tgcgtaacca tatccgtttg ctgggacgca aagattatca
    15001 acagctgccg gggctgcgaa ccctggatta cgtgctgatg tttattcccg ttgaacccgc
    15061 ttttttactg gcgcttgacc gccagccgga gctgatcacc gaagcgttga aaaacaatat
    15121 catgctggtt agcccgacta cgctgctggt ggcgctgcgc actatcgcca acctgtggcg
    15181 ttatgagcat caaagccgca acgcccagca aatcgccgat cgtgccagca agctgtacga
    15241 caagatgcgt ttgttcgtcg atgacatgtc agcgattggt caaagtctcg acaaagcgca
    15301 ggataactat cgccaggcaa tgaaaaaact ctcttcaggg cgcggaaatg tgctggcgca
    15361 ggcagaagcg tttcgcggtt taggagtaga aattaaacgc gagattaatc cggatttggc
    15421 tgaacaggcg gtgagccagg atgaagagta tcgacttcgg tcggttccgg agcagccgaa
    15481 tgatgaagct tatcaacgcg atgatgaata taatcagcag tcgcgctagc ccattgggag
    15541 tagttaagcc gggtagaaat ctagggcatc gacgcccaat ctgttacact tctggaacaa
    15601 ttttttgatg agcaggcatt gagatggtgg ataagtcaca agaaacgacg cactttggtt
    15661 ttcagaccgt cgcgaaggaa caaaaagcgg atatggtcgc ccacgttttc cattccgtgg
    15721 catcaaaata cgatgtcatg aatgatttga tgtcatttgg tattcatcgt ttgtggaagc
    15781 gattcacgat tgattgcagc ggcgtacgcc gtgggcagac cgtgctggat ctggctggtg
    15841 gcaccggcga cctgacagcg aaattctccc gcctggtcgg agaaactggc aaagtggtcc
    15901 ttgctgatat caatgaatcc atgctcaaaa tgggccgcga gaagctgcgt aatatcggtg
    15961 tgattggcaa cgttgagtat gttcaggcga acgctgaggc gctgccgttc ccggataaca
    16021 cctttgattg catcaccatt tcgtttggtc tgcgtaacgt caccgacaaa gataaagcac
    16081 tgcgttcaat gtatcgcgtg ctgaaacccg gcggccgcct gctggtgctt gagttctcga
    16141 agccaattat cgagccgctg agcaaagcct atgatgcata ctccttccat gtgctgccgc
    16201 gtattggctc actggtcgcg aacgacgccg acagctaccg ttatctggca gaatccatcc
    16261 gtatgcatcc cgatcaggat accctgaaag ccatgatgca ggatgccgga ttcgaaagtg
    16321 tcgactacta caatctgacg gcaggggttg tggcgctgca tcgtggttat aagttctgac
    16381 aggagaccgg aaatgccttt taaaccttta gtgacggcag gaattgaaag tctgctcaac
    16441 accttcctgt atcgctcacc cgcgctgaaa acggcccgct cgcgtctgct gggtaaagta
    16501 ttgcgcgtgg aggtaaaagg cttttcgacg tcattgattc tggtgttcag cgaacgccag
    16561 gttgatgtac tgggcgaatg ggcaggcgat gctgactgca ccgttatcgc ctacgccagt
    16621 gtgttgccga aacttcgcga tcgccagcag cttaccgcac tgattcgcag tggtgagctg
    16681 gaagtgcagg gcgatattca ggtggtgcaa aacttcgttg cgctggcaga tctggcagag
    16741 ttcgaccctg cggaactgct ggccccttat accggtgata tcgccgctga aggaatcagc
    16801 aaagccatgc gcggaggcgc aaagttcctg catcacggca ttaagcgcca gcaacgttat
    16861 gtggcggaag ccattactga agagtggcgt atggcacccg gtccgcttga agtggcctgg
    16921 tttgcggaag agacggctgc cgtcgagcgt gctgttgatg ccctgaccaa acggctggaa
    16981 aaactggagg ctaaatgacg ccaggtgaag tacggcgcct atatttcatc attcgcactt
    17041 ttttaagcta cggacttgat gaactgatcc ccaaaatgcg tatcaccctg ccgctacggc
    17101 tatggcgata ctcattattc tggatgccaa atcggcataa agacaaactt ttaggtgagc
    17161 gactacgact ggccctgcaa gaactggggc cggtttggat caagttcggg caaatgttat
    17221 caacccgccg cgatcttttt ccaccgcata ttgccgatca gctggcgtta ttgcaggaca
    17281 aagttgctcc gtttgatggc aagctggcga agcagcagat tgaagctgca atgggcggct
    17341 tgccggtaga agcgtggttt gacgattttg aaatcaagcc gctggcttct gcttctatcg
    17401 cccaggttca taccgcgcga ttgaaatcga atggtaaaga ggtggtgatt aaagtcatcc
    17461 gcccggatat tttgccggtc attaaagcgg atctgaaact tatctaccgt ctggctcgct
    17521 gggtgccgcg tttgctgccg gatggtcgcc gtctgcgccc aaccgaagtg gtgcgcgagt
    17581 acgaaaagac cttgattgat gaactgaatt tgctgcggga atctgccaat gccattcagc
    17641 ttcggcgcaa ttttgaagac agcccgatgc tctacatccc ggaagtttac cctgactatt
    17701 gtagtgaagg gatgatggtg atggagcgca tttacggcat tccggtgtct gatgttgcgg
    17761 cgctggagaa aaacggcact aacatgaaat tgctggcgga acgcggcgtg caggtgttct
    17821 tcactcaggt tttccgtgac agctttttcc atgccgatat gcaccctggc aacatcttcg
    17881 taagctatga acacccggaa aacccgaaat atatcggcat tgattgcggg attgttggct
    17941 cgctaaacaa agaagataaa cgctatctgg cagaaaactt tatcgccttc tttaatcgcg
    18001 actatcgcaa agtggcagag ctacacgtcg attcaggctg ggtgccacca gataccaacg
    18061 ttgaagagtt cgaatttgcc attcgtacgg tctgtgaacc tatctttgag aaaccgctgg
    18121 ccgaaatttc gtttggacat gtactgttaa atctgtttaa tacggcgcgt cgcttcaata
    18181 tggaagtgca gccgcaactg gtgttactcc agaaaaccct gctctatgtc gaaggggtag
    18241 gacgccagct ttatccgcag ctcgatttat ggaaaacagc gaagcctttc ctggagtcgt
    18301 ggattaaaga tcaggtcggt attcctgcgc tggtgagagc atttaaagaa aaagcgccgt
    18361 tctgggtcga aaaaatgcca gaactgcctg aattggttta cgacagtttg cgccagggca
    18421 agtatttaca gcacagtgtt gataagattg cccgcgagct tcagtcaaat catgtacgtc
    18481 agggacaatc gcgttatttt ctcggaattg gcgctacgtt agtattaagt ggcacattct
    18541 tgttggtcag ccgacctgaa tgggggctga tgcccggctg gttaatggca ggtggtctga
    18601 tcgcctggtt tgtcggttgg cgcaaaacac gctgattttt tcatcgctca aggcgggccg
    18661 tgtaacgtat aatgcggctt tgtttaatca tcatctacca cagaggaaca tgtatgggtg
    18721 gtatcagtat ttggcagtta ttgattattg ccgtcatcgt tgtactgctt tttggcacca
    18781 aaaagctcgg ctccatcggt tccgatcttg gtgcgtcgat caaaggcttt aaaaaagcaa
    18841 tgagcgatga tgaaccaaag caggataaaa ccagtcagga tgctgatttt actgcgaaaa
    18901 ctatcgccga taagcaggcg gatacgaatc aggaacaggc taaaacagaa gacgcgaagc
    18961 gccacgataa agagcaggtg taatccgtgt ttgatatcgg ttttagcgaa ctgctattgg
    19021 tgttcatcat cggcctcgtc gttctggggc cgcaacgact gcctgtggcg gtaaaaacgg
    19081 tagcgggctg gattcgcgcg ttgcgttcac tggcgacaac ggtgcagaac gaactgaccc
    19141 aggagttaaa actccaggag tttcaggaca gtctgaaaaa ggttgaaaag gcgagcctca
    19201 ctaacctgac acccgaactg aaagcgtcga tggatgaact acgccaggcc gcggagtcga
    19261 tgaagcgttc ctacgttgca aacgatcctg aaaaggcgag cgatgaagcg cacaccatcc
    19321 ataacccggt ggtgaaagat aatgaagctg cgcatgaggg cgtaacgcct gccgctgcac
    19381 aaacgcaggc cagttcgccg gaacagaagc cagaaaccac gccagagccg gtggtaaaac
    19441 ctgctgcgga cgctgaaccg aaaaccgctg caccttcccc ttcgtcgagt gataaaccgt
    19501 aaacatgtct gtagaagata ctcaaccgct tatcacgcat ctgattgagc tgcgtaagcg
    19561 tctgctgaac tgcattatcg cggtgatcgt gatattcctg tgtctggtct atttcgccaa
    19621 tgacatctat cacctggtat ccgcgccatt gatcaagcag ttgccgcaag gttcaacgat
    19681 gatcgccacc gacgtggcct cgccgttctt tacgccgatc aagctgacct ttatggtgtc
    19741 gctgattctg tcagcgccgg tgattctcta tcaggtgtgg gcatttatcg ccccagcgct
    19801 gtataagcat gaacgtcgcc tggtggtgcc gctgctggtt tccagctctc tgctgtttta
    19861 tatcggcatg gcgttcgcct actttgtggt ctttccgctg gcatttggct tccttgccaa
    19921 taccgcgccg gaaggggtgc aggtatccac cgacatcgcc agctatttaa gcttcgttat
    19981 ggcgctgttt atggcgtttg gtgtctcctt tgaagtgccg gtggcaattg tgctgctgtg
    20041 ctggatgggg attacctcgc cagaagactt acgcaaaaaa cgcccgtatg tgctggttgg
    20101 tgcattcgtt gtcgggatgt tgctgacgcc gccggatgtc ttctcgcaaa cgctgttggc
    20161 gatcccgatg tactgtctgt ttgaaatcgg tgtcttcttc tcacgctttt acgttggtaa
    20221 agggcgaaat cgggaagagg aaaacgacgc tgaagcagaa agcgaaaaaa ctgaagaata
    20281 aattcaaccg cccgtcaggg cggttgtcat atggagtaca ggatgtttga tatcggcgtt
    20341 aatttgacca gttcgcaatt tgcgaaagac cgtgatgatg ttgtagcgcg cgcttttgac
    20401 gcgggagtta atgggctact catcaccggc actaacctgc gtgaaagcca gcaggcgcaa
    20461 aagctggcgc gtcagtattc gtcctgttgg tcaacggcgg gcgtacatcc tcacgacagc
    20521 agccagtggc aagctgcgac tgaagaagcg attattgagc tggccgcgca gccagaagtg
    20581 gtggcgattg gtgaatgtgg tctcgacttt aaccgcaact tttcgacgcc ggaagagcag
    20641 gaacgcgctt ttgttgccca gctacgcatt gccgcagatt taaacatgcc ggtatttatg
    20701 cactgtcgcg atgcccacga gcggtttatg acattgctgg agccgtggct ggataaactg
    20761 cctggtgcgg ttcttcattg ctttaccggc acacgcgaag agatgcaggc gtgcgtggcg
    20821 catggaattt atatcggcat taccggttgg gtttgcgatg aacgacgcgg actggagctg
    20881 cgggaacttt tgccgttgat tccggcggaa aaattactga tcgaaactga tgcgccgtat
    20941 ctgctccctc gcgatctcac gccaaagcca tcatcccggc gcaacgagcc agcccatctg
    21001 ccccatattt tgcaacgtat tgcgcactgg cgtggagaag atgccgcatg gctggctgcc
    21061 accacggatg ctaatgtcaa aacactgttt gggattgcgt tttagagttt gcggaactcg
    21121 gtgttcttca cactgtgctt aatctcttta ttaataagat taagcaatag catggagcga
    21181 gcctcaccat cgggttcggt gaaaatggcc tgaaagcctt cgaacgcgcc ttcggtaata
    21241 atcaccttat ctcccggata aggggttgcc ggatcgacaa tgtctttcgg tttatatacc
    21301 gatagctgat gaatcaccgc cgatgggact atcgctggcg acgcgccaaa gcgcacgaag
    21361 tgactgacac cgcgggtcgc gttgatagtc gtggtatgaa tcacttctgg atcaaattcg
    21421 acaaacaggt agttgggaaa caatggctca ctgactgcag tacgttttcc acgcacgatt
    21481 ttttccaggg tgatcatcgg tgccaggcaa ttcacagcct gtctttcgag gtgttcctgg
    21541 gcacgttgaa gttgcccgcg cttgcagtac agtaaatacc aggattgcat aatgactctt
    21601 atccgtttaa tcggggcgca aggatagcaa aagctttacg ctaagttaat tatattcccc
    21661 ggtttgcgtt ataccgtcag agttcacgct aatttaacaa atttacagca tcgcaaagat
    21721 gaacgccgta taatgggcgc agattaagag gctacaatgg acgccatgaa atataacgat
    21781 ttacgcgact tcttgacgct gcttgaacag cagggtgagc taaaacgtat cacgctcccg
    21841 gtggatccgc atctggaaat cactgaaatt gctgaccgca ctttgcgtgc cggtgggcct
    21901 gcgctgttgt tcgaaaaccc taaaggctac tcaatgccgg tgctgtgcaa cctgttcggt
    21961 acgccaaagc gcgtggcgat gggcatgggg caggaagatg tttcggcgct gcgtaaagtt
    22021 ggtaaattat tggcgtttct gaaagagccg gagccgccaa aaggtttccg cgacctgttt
    22081 gataaactgc cgcagtttaa gcaagtattg aacatgccga caaagcggct gcgtggtgcg
    22141 ccctgccaac aaaaaatcgt ctctggcgat gacgtcgatc tcaatcgcat tcccattatg
    22201 acctgctggc cggaagatgc cgcgccgctg attacctggg ggctgacagt gacgcgcggc
    22261 ccacataaag agcggcagaa tctgggcatt tatcgccagc agctgattgg taaaaacaaa
    22321 ctgattatgc gctggctgtc gcatcgcggc ggcgcgctgg attatcagga gtggtgtgcg
    22381 gcgcatccgg gcgaacgttt cccggtttct gtggcgctgg gtgccgatcc cgccacgatt
    22441 ctcggtgcag tcactcccgt tccggatacg ctttcagagt atgcgtttgc cggattgcta
    22501 cgtggcacca agaccgaagt ggtgaagtgt atctccaatg atcttgaagt gcccgccagt
    22561 gcggagattg tgctggaagg gtatatcgaa caaggcgaaa ctgcgccgga agggccgtat
    22621 ggcgaccaca ccggttacta taatgaagtc gatagtttcc cggtatttac cgtgacgcat
    22681 attacccagc gtgaagatgc gatttaccat tccacctata ccgggcgtcc gccagatgag
    22741 cccgcggtgc tgggtgtcgc actgaacgaa gtgtttgtgc cgattctgca aaaacagttc
    22801 ccggaaattg tcgattttta cctgccgccg gaaggctgct cttatcgcct ggcggtagtg
    22861 acaatcaaaa aacagtacgc cggacacgcg aagcgcgtca tgatgggcgt ctggtcgttc
    22921 ttacgccagt ttatgtacac taaatttgtg atcgtttgcg atgatgacgt taacgcacgc
    22981 gactggaacg atgtgatttg ggcgattacc acccgtatgg acccggcgcg ggatactgtt
    23041 ctggtagaaa atacgcctat tgattatctg gattttgcct cgcctgtctc cgggctgggt
    23101 tcaaaaatgg ggctggatgc cacgaataaa tggccggggg aaacccagcg tgaatgggga
    23161 cgtcccatca aaaaagatcc agatgttgtc gcgcatattg acgccatctg ggatgaactg
    23221 gctattttta acaacggtaa aagcgcctga tgcgcgtttg ttttgcccta tttatcgatc
    23281 cgacagagaa agcgcatgac aaccttaagc tgtaaagtga cctcggtaga agctatcacg
    23341 gataccgtat atcgtgtccg catcgtgcca gacgcggcct tttcttttcg tgctggtcag
    23401 tatttgatgg tagtgatgga tgagcgcgac aaacgtccgt tctcaatggc ttcgacgccg
    23461 gatgaaaaag ggtttatcga gctgcatatt ggcgcttctg aaatcaacct ttacgcgaaa
    23521 gcagtcatgg accgcatcct caaagatcat caaatcgtgg tcgacattcc ccacggagaa
    23581 gcgtggctgc gcgatgatga agagcgtccg atgattttga ttgcgggcgg caccgggttc
    23641 tcttatgccc gctcgatttt gctgacagcg ttggcgcgta acccaaaccg tgatatcacc
    23701 atttactggg gcgggcgtga agagcagcat ctgtatgatc tctgcgagct tgaggcgctt
    23761 tcgttgaagc atcctggtct gcaagtggtg ccggtggttg aacaaccgga agcgggctgg
    23821 cgtgggcgta ctggcaccgt gttaacggcg gtattgcagg atcacggtac gctggcagag
    23881 catgatatct atattgccgg acgttttgag atggcgaaaa ttgcccgcga tctgttttgc
    23941 agtgagcgta atgcgcggga agatcgcctg tttggcgatg cgtttgcatt tatctgagat
    24001 ataaaaaaac ccgcccctga caggcgggaa gaacggcaac taaactgtta ttcagtggca
    24061 tttagatcta tgacgtatct ggcaaaccat gcccgatgcg acgctgtcgc gtcttatcgt
    24121 gcctacaaat agtccgaacc gtaggccgga taaggcgttt acgccgcatc cggcaattgg
    24181 tgcatgatgc ctgatgcgac gctggcgcgt cttatcaggc ctacattggt gccggatcgg
    24241 tagaccggat aaggcgttca cgccgcatcc ggcaagtggt taaacccgct caaacaccgt
    24301 cgcaataccc tgacccagac cgatacacat cgtcgccaga ccaaactgaa cgtctttgcg
    24361 ttccatcaga ttcagcagcg tggtgctgat acgcgcaccg gaacaaccca gcggatgacc
    24421 cagcgcgatc gcgccaccgt tgaggttgat cttctcgtca atctgctcaa ttagtcccag
    24481 atctttaata catggcagga tctgcgcggc aaaggcttcg ttcatttcaa acacgccgat
    24541 atcgctggca gaaagccccg cttttttcag cgccagtttc gaggccggaa ccgggccgta
    24601 acccataatc gatgggtcac aaccaacgac cgccatcgaa cgcacacgag cgcgcggctt
    24661 aagacctaat tcatgggcgc ggctttcact catcaccagc atggcagctg cgccatcgga
    24721 aagtgcagaa gatgtgcccg ccgttaccat accgtttact ggatcaaacg ccggacgcag
    24781 cgtggcgagg gcttccacgg tggtttccgg gcgaatcact tcgtcgtaat taaactgctt
    24841 caggacgccg tcggcatcgt gaccaccggt cgggatgatt tcatttttaa atgcggccga
    24901 ctgcgtggcg gcccaggcgc gggcgtgtga ccgcgcggca aaggcatcct gcatttcacg
    24961 gctgataccg tgcatacgcg ccagcatttc tgccgttaag cccatcatgc ccgccgcttt
    25021 ggcgacattg cggctcaggc cggggtgaaa atcgacgccg tgactcatcg gcacatggcc
    25081 catatgctcc acgccgccaa ccagacatgc ctgcgcatcg ccagtcatga tcattcgtgc
    25141 tgcgtcatgc agtgcctgca tggatgaacc acacaagcga ttaacggtaa ccgccgggac
    25201 agagtgtggt acttctgcca gcagcgccgc gttacgggcg atattaaaac cctgctccag
    25261 cgtctgctgc acacaacccc agtaaatatc gtcgagggcc gccgcttcca gcgccgggtt
    25321 acgcgccagc aggctacgca ttaaatgagc ggagagatct tctgcacgca cgttacgaaa
    25381 agcaccgccc ttcgaacggc ccatcggggt gcgaattgca tcgacaatga caacctgttc
    25441 cattgtgact ccttaagccg ttttcaggtc gccaaccgga cgggctggct caaccggagg
    25501 atagtacggt tcgttatgac gcgctttatt acgcagacct tccggcactt catacagcgg
    25561 gccgaggtgc tgatattgct gtgccatatc gaggtatttt gcgctaccga gggtgtccag
    25621 ccagcggaac gcgccgccgt ggaacggagg gaagcccagg ccgtagacca gcgccatatc
    25681 cgcttccgcc ggagtggcga taatgccttc ctccagacag cgcaccactt cgttgaccat
    25741 cgggatcatc atgcgggcga taatctcttc ttcgctgaaa tcgcgcttcg gctggctcac
    25801 ttctgccagc aggtcttcaa cggcggcgtc ttcttctttc ttcggcttac ctttgctgtc
    25861 ttctttataa cgccagaaac cgaggccgtt cttctgacca aagcggttgg catcaaacag
    25921 cgcgtcgatg gcatcgcggt aatctttctg catccgctgc gggaagcctg ctgccatgac
    25981 agcctgagcg tgatgcgcgg tatcaatgcc cacaacgtcc agcagatatg ccgggcccat
    26041 cggccagcca aactgttttt ccatcacttt gtcgatcttg cggaaatccg cgccgtcgcg
    26101 cagcagctgg ctgaaaccgg cgaaatacgg gaacagcacg cggttaacaa agaagccggg
    26161 gcagtcgtta accacaatcg gcgtcttgcc catcttgctc gcccaggcga caactttcgc
    26221 gatggtttcg tcggagcttt tctcgccgcg aataatttct accaacggca ttcggtggac
    26281 cgggttaaag aagtgcatcc cgcagaagtt ttccgggcgt tccagcgcgt tggccagttc
    26341 gctgatagga atggttgaag tgttagacgc cagcacggta tcctggcgta ctttttgttc
    26401 ggtttctgcc agtacggctt ttttcacttt cgggttttca acaaccgctt ctaccacaat
    26461 atccacgcgg tcaaatccgg cgtagtcgag cgttgggtgg attgtggaga tcacgccagc
    26521 cagtttcaga ccatcgatct tgccgcgctc aagctgcttg ttcagcagtt tcgcggcttc
    26581 ggtcatgccg agggttaacg acttgtcgtt gatatctttc atgacaaccg gcacgccttt
    26641 ccacgcagac tggtaagcga tgccgccgcc cataatgcct gcacccagca ccgcggcctg
    26701 tttcggggtt tcaacgtctt tggtgagttt cttcgctttg ccttttacat attgatcgtt
    26761 aaggaaaatg ccgaccagtg cgcgggcttc gttggtatgc gccagcggga caaaactttt
    26821 gttttccagg tttaaggctt cttcacgacc aaaacgggcc gcagcttcaa tggtttttac
    26881 tgcggtgatg ggggccggat aatgtttccc cgctgtttgt gcgaccatcc ctttagcgat
    26941 ggtgaagctc atggtggctt caatcttgct cagttttagt ggttccagct tcggctgacg
    27001 ttttgctttc cagtcgaggt cgccgttaat ggcctggcgt aaaaccgcct ttgcgccttc
    27061 aaccagtttt tctgctttga ctacgccatc caccagaccg attttcagcg cctgatccgc
    27121 gccgacatct ttaccggcgg caatgatttc cagcgcactg tcagcgccca gcatacgtgg
    27181 catacgtaca gaaccgccaa agccaggcat gatgcccagt ttggtttccg gcagaccgat
    27241 gcgcagatcc ggcgtcgcca gacgataatc ggtcgccagc acgcattcgc agccaccgcc
    27301 cagcgcatag ccattgacgg cagcaatggt cggcaccggc agatcttcca ggcgattaaa
    27361 cacgctattg gcaaagtgca gccactgact taactgttct tcaggaacga ggaacaggga
    27421 caaaaattcg gtgatatcag caccgacgat aaaggctgct ttgttcgaac gcagcagcag
    27481 cccttttaga tctgattgct gttccagcac gccgatggcc tcgccgaggc tggcgacggt
    27541 cgcagtgtcg agtttattaa ctgaacctgg ggcatcaaat accagttcgg caatgccatc
    27601 ttccagccag tcaaggtaca gggtgtcgcc tttgtaaagc atgtcagtct cctgaatccg
    27661 caaggtgatc tggtcgtacc agatgagtcg aagtgtgtat tttgtgttaa aaatatgcaa
    27721 acaaaagatt aaagaaatgc cgatctgatc acgctcggca gaaatcacgc tctggatgaa
    27781 cgatgtgcta agatgcggag acttaaggtc aaaaaaacag aagggtaaaa aatggaatca
    27841 ctggcctcgc tctataaaaa tcatatagct accttacaag aacggactcg cgatgcgctg
    27901 gcgcgcttca agctggatgc gttacttatt cactccggcg agctgttcaa tgtttttctc
    27961 gacgatcatc cctatccgtt taaagtgaac ccgcaattca aagcgtgggt gccggtaact
    28021 caggtgccaa actgctggtt gctggtggat ggcgtgaaca agccgaaact gtggttctat
    28081 ctgccggttg attactggca caacgtcgaa ccgctgccga cctctttctg gactgaagat
    28141 gtggaagtga tcgcgctgcc gaaagccgat ggcattggta gcctgctgcc tgctgcgcgc
    28201 ggcaatatcg gttatatcgg tccggtgccg gaacgtgcgc tgcaactggg tattgaggcc
    28261 agcaacatca acccgaaagg ggttatcgac tacctgcatt actatcgctc cttcaaaact
    28321 gagtacgaac tggcctgtat gcgtgaagcg cagaaaatgg cggtcaacgg tcaccgcgcg
    28381 gcagaagaag cgttccgttc tggcatgagc gagttcgata tcaacattgc ctatctgacc
    28441 gcgaccggtc atcgtgatac cgacgtacct tacagcaaca ttgtggcgct taacgaacac
    28501 gctgcggtac tgcattacac caaactggac catcaggcac cggaagagat gcgcagcttc
    28561 ctgctggatg ccggggcaga atataacggc tatgcggctg acctgacccg tacctggtcg
    28621 gcaaaaagtg acaacgacta cgcgcagctg gtgaaggacg ttaatgatga acaactggcg
    28681 ctgatcgcca ccatgaaagc aggcgtcagc tatgtggatt accacatcca gttccatcag
    28741 cgcatcgcca aactgctgcg taaacatcaa atcatcaccg atatgagtga agaagcgatg
    28801 gtcgaaaacg atcttaccgg gccgtttatg ccgcatggta tcggccatcc gctgggcctg
    28861 caggtgcatg acgtcgctgg ttttatgcag gatgatagcg gtacgcacct cgcggcaccg
    28921 gcaaaatatc cgtacctgcg ctgcacccgt attctccagc cgggcatggt gttaaccatc
    28981 gaaccgggta tctacttcat cgaatcgcta ctggcaccgt ggcgtgaagg gcagttcagc
    29041 aagcacttca actggcagaa aattgaagca ctgaaaccgt tcggcggcat tcgtatcgaa
    29101 gacaacgtgg tgatccacga aaacaacgtg gaaaacatga cccgggatct gaaactggcg
    29161 tgatggaaag ctggttaatt cctgcggcac cggtcacggt cgttgaagag atcaaaaaga
    29221 gccgcttcat aacgatgttg gcgcataccg atggcgttga ggcggcgaaa gcgtttgttg
    29281 aatcggtgcg ggcagaacac cccgatgccc ggcaccattg cgtggcgtgg gtcgcgggtg
    29341 cgccggatga ttctcaacag ctgggtttct ctgacgacgg ggagccggcg ggaacggcag
    29401 gtaaaccgat gctcgcccag ctaatgggca gcggcgtcgg ggaaattacc gctgtggtag
    29461 tgcgctacta cggcggcata ttgcttggca ccggtgggtt agtgaaagcg tatggcggcg
    29521 gcgtgaatca ggcgctgcgc cagctaacga cccaacgcaa gacgccatta accgaatata
    29581 ctttgcaatg tgaatatcat cagttaaccg gcattgaagc gttgctgggg cagtgtgacg
    29641 gcaaaattat caacagtgat tatcaggcat tcgttctgct gcgggtggcg cttccggcgg
    29701 cgaaagtggc tgaattttcc gcaaagctgg cggattttag ccgtggttca ttgcaattgt
    29761 tagcgattga agaataatcc ccacttcgtt ttgcagaact aaggaagcgg cagagatgca
    29821 ttttcgcgcc attacccgaa tcgttggact actggtcatc ttattttcag ggaccatgat
    29881 tatccctggg ctggtagcac tcatctaccg ggatggagcg ggccgcgctt ttacccagac
    29941 cttttttgtc gccctcgcca ttggctctat gctgtggtgg ccgaaccgca aagagaaagg
    30001 cgaacttaaa tcccgtgagg ggtttctgat agtggtgctg ttctggaccg tgctgggtag
    30061 cgtcggtgcg ctccctttta tcttctcgga aagcccgaac ctcacgatta ccgatgcgtt
    30121 ttttgaatct ttctctggcc tgaccactac gggagccact acgctggtgg ggctggattc
    30181 gctccctcac gccatcctct tttatcgcca gatgctgcaa tggtttggcg ggatggggat
    30241 catcgtgttg gcggttgcga tactgcctat cctcggcgtg ggtgggatgc agctctatcg
    30301 cgcagaaatg cccggcccgc tgaaagataa caaaatgcgc ccgcgaattg cggaaacggc
    30361 gaaaaccctg tggttgattt atgtcttgct gaccgtcgcc tgtgcgctgg cgttgtggtt
    30421 tgccggaatg gatgcctttg atgccatcgg ccatagcttt gcgactatcg ctattggcgg
    30481 cttctcgaca catgatgcca gtatcggtta tttcgatagc ccgactatta acactatcat
    30541 tgctatcttc ctgctgatct ccggctgtaa ctacggtctg cacttttcac tgttaagtgg
    30601 gcgtagtctg aaggtttatt ggcgcgatcc ggaatttcgc atgtttatcg gcgtacagtt
    30661 tacgctggtg gttatttgta ccctcgtact gtggtttcat aatgtctaca gttcggcgct
    30721 gatgacaatt aaccaggcgt ttttccaggt ggtgtcgatg gcgacaaccg ccgggtttac
    30781 aactgacagc attgcccgct ggccgctctt tttgccggta ctgcttttat gttcagcctt
    30841 tatcggcggt tgcgccgggt caacgggcgg tggcctgaag gtgatccgca tcctgctgct
    30901 gtttaagcag gggaaccgtg agctgaaacg actggtgcat ccgaacgccg tctatagcat
    30961 taagctgggg aatcgcgcac tgccggaacg tatcctcgaa gccgtttggg gatttttctc
    31021 cgcctatgca ttggtgttta ttgtcagtat gctggcgatt atcgccacgg gcgtggatga
    31081 cttttctgcc tttgcgtcgg ttgttgcgac attgaataac ctggggccag ggcttggcgt
    31141 ggttgctgat aactttacca gtatgaaccc ggtggctaaa tggatcctga ttgccaacat
    31201 gctgtttggt cgtctcgagg tctttacatt gctggtgctc tttaccccga ctttctggcg
    31261 tgaatgatgg agtaatacgt gaaaacatta attcttttct caacaaggga cggacaaacg
    31321 cgcgagattg cctcctacct ggcttcggaa ctgaaagaac tggggatcca ggcggatgtc
    31381 gccaatgtgc accgcattga agaaccacag tgggaaaact atgaccgtgt ggtcattggt
    31441 gcttctattc gctatggtca ctaccattca gcgttccagg aatttgtcaa aaaacatgcg
    31501 acgcggctga attcgatgcc gagcgccttt tactccgtga atctggtggc gcgcaaaccg
    31561 gagaagcgta ctccacagac caacagctac gcgcgcaagt ttctgatgaa ctcgcaatgg
    31621 cgtcccgatc gctgcgcggt cattgccggg gcgctgcgtt acccacgtta tcgctggtac
    31681 gaccgtttta tgatcaagct gattatgaag atgtcaggcg gtgaaacgga tacgcgcaaa
    31741 gaagttgtct ataccgattg ggagcaggtg gcgaatttcg cccgagaaat cgcccattta
    31801 accgacaaac cgacgctgaa ataagcataa agaa
//
LOCUS       DAJOSY010000050        29489 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJOSY010000050 DAJOSY010000000
VERSION     DAJOSY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.050"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..95
                     /locus_tag="OHN27_002334"
     ncRNA           <1..95
                     /ncRNA_class="other"
                     /locus_tag="OHN27_002334"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(279..956)
                     /gene="narI"
                     /locus_tag="OHN27_002335"
     CDS             complement(279..956)
                     /gene="narI"
                     /locus_tag="OHN27_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCQ2286765.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(956..1666)
                     /gene="narJ"
                     /locus_tag="OHN27_002336"
     CDS             complement(956..1666)
                     /gene="narJ"
                     /locus_tag="OHN27_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCQ2286766.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1663..3201)
                     /gene="narH"
                     /locus_tag="OHN27_002337"
     CDS             complement(1663..3201)
                     /gene="narH"
                     /locus_tag="OHN27_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCQ2286767.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3198..6941)
                     /locus_tag="OHN27_002338"
     CDS             complement(3198..6941)
                     /locus_tag="OHN27_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCQ2286768.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7457..8848)
                     /gene="narK"
                     /locus_tag="OHN27_002339"
     CDS             complement(7457..8848)
                     /gene="narK"
                     /locus_tag="OHN27_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCQ2286769.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9187..10983
                     /gene="narX"
                     /locus_tag="OHN27_002340"
     CDS             9187..10983
                     /gene="narX"
                     /locus_tag="OHN27_002340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCQ2286770.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10976..11626
                     /gene="narL"
                     /locus_tag="OHN27_002341"
     CDS             10976..11626
                     /gene="narL"
                     /locus_tag="OHN27_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCQ2286771.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11627..13021)
                     /gene="ychO"
                     /locus_tag="OHN27_002342"
     CDS             complement(11627..13021)
                     /gene="ychO"
                     /locus_tag="OHN27_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCQ2286772.1"
                     /translation="MSRFVPRIISFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            13207..13560
                     /gene="ychN"
                     /locus_tag="OHN27_002343"
     CDS             13207..13560
                     /gene="ychN"
                     /locus_tag="OHN27_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCQ2286773.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13604..14299)
                     /gene="chaC"
                     /locus_tag="OHN27_002344"
     CDS             complement(13604..14299)
                     /gene="chaC"
                     /locus_tag="OHN27_002344"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCQ2286774.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14457..14687)
                     /gene="chaB"
                     /locus_tag="OHN27_002345"
     CDS             complement(14457..14687)
                     /gene="chaB"
                     /locus_tag="OHN27_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCQ2286775.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14957..16057
                     /gene="chaA"
                     /locus_tag="OHN27_002346"
     CDS             14957..16057
                     /gene="chaA"
                     /locus_tag="OHN27_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCQ2286776.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16462..16569
                     /locus_tag="OHN27_002347"
     CDS             16462..16569
                     /locus_tag="OHN27_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCQ2286777.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGITTAAIVGWWRNRK"
     gene            complement(16718..17572)
                     /gene="kdsA"
                     /locus_tag="OHN27_002348"
     CDS             complement(16718..17572)
                     /gene="kdsA"
                     /locus_tag="OHN27_002348"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCQ2286778.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(17608..18417)
                     /locus_tag="OHN27_002349"
     CDS             complement(17608..18417)
                     /locus_tag="OHN27_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCQ2286779.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(18421..18813)
                     /locus_tag="OHN27_002350"
     CDS             complement(18421..18813)
                     /locus_tag="OHN27_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCQ2286780.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(18810..19643)
                     /gene="prmC"
                     /locus_tag="OHN27_002351"
     CDS             complement(18810..19643)
                     /gene="prmC"
                     /locus_tag="OHN27_002351"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCQ2286781.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IRILQSDWFSALAGQQFTMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(19643..20725)
                     /gene="prfA"
                     /locus_tag="OHN27_002352"
     CDS             complement(19643..20725)
                     /gene="prfA"
                     /locus_tag="OHN27_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCQ2286782.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(20767..22023)
                     /gene="hemA"
                     /locus_tag="OHN27_002353"
     CDS             complement(20767..22023)
                     /gene="hemA"
                     /locus_tag="OHN27_002353"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCQ2286783.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22237..22860
                     /gene="lolB"
                     /locus_tag="OHN27_002354"
     CDS             22237..22860
                     /gene="lolB"
                     /locus_tag="OHN27_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCQ2286784.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22860..23711
                     /gene="ispE"
                     /locus_tag="OHN27_002355"
     CDS             22860..23711
                     /gene="ispE"
                     /locus_tag="OHN27_002355"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCQ2286785.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADLSIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            23862..24809
                     /gene="prs"
                     /locus_tag="OHN27_002356"
     CDS             23862..24809
                     /gene="prs"
                     /locus_tag="OHN27_002356"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCQ2286786.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            24934..26613
                     /gene="dauA"
                     /locus_tag="OHN27_002357"
     CDS             24934..26613
                     /gene="dauA"
                     /locus_tag="OHN27_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCQ2286787.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(26668..26946)
                     /gene="ychH"
                     /locus_tag="OHN27_002358"
     CDS             complement(26668..26946)
                     /gene="ychH"
                     /locus_tag="OHN27_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCQ2286788.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27224..27808
                     /gene="pth"
                     /locus_tag="OHN27_002359"
     CDS             27224..27808
                     /gene="pth"
                     /locus_tag="OHN27_002359"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCQ2286789.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            27925..29016
                     /gene="ychF"
                     /locus_tag="OHN27_002360"
     CDS             27925..29016
                     /gene="ychF"
                     /locus_tag="OHN27_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCQ2286790.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         7113 a         7772 c         7400 g         7204 t
ORIGIN      
        1 aaggttcgac ttgagcgcca gcgagagagc gttgccgcag gcaacgaccc gaagggcgaa
       61 gcgcgcagcg ctgagtaatc cttcccccac caccatcgct tccttaaata atcaaaacct
      121 gttactccga gctaattttg cccgatggca ccgcgtttac cggacctaca atatcctccc
      181 ctgtaggtcg gataagcgaa gcgcatccga caactcaccc cgcaccgtgc ccataaaaaa
      241 accccgccga agcagggtct atgtgaacta aaattcgctt agtgacgagc gcgcaccaac
      301 tgatacttac gtgtcagata ctccaccggt acgctccaga tgtgcaccag acgcgagaac
      361 gggaacagca ggaataacgt catccccaac accaggtgca gacggaagat aaacgctaca
      421 ccatcaaggt gttgagaagc gccaccgtgg aaggtcacta ccgactgcgc ccagccaacc
      481 agtttcatca tctcgctacc gtccatatgc tgagcggaga acggaatggt caacaggccc
      541 agcgcgcact ggataacgag cagcgacagg atcaggatat ccgctccggt agtggttgca
      601 cgcacgcgtg ggctgaacag acgacgcttc agcaacagca cgccgccaat cagacacagc
      661 acgccgctgg caccaccagc aaacatcgcc attttctgtt tcacttcaat cggcagccag
      721 gcttcataca tccagtgcgg cgtcagcata ccgaagaagt gaccaacaaa aatccccaga
      781 ataccgatat ggaacaggtt cgacgccagg ttcatccctt tgcgatccag catctggctg
      841 gacgccgcgc gccaggtgta ctgcccgtag tcataacgca gccagctacc aatcaggaag
      901 accgccccgg ctatgtacgg gtagatatca aagaagaaca tattcaggaa ttgcattagt
      961 gctgtcctcc ggtggtgata ttcagatatt gcggcgcgac ggcaccggca aagcgacgct
     1021 gatgagcagt gattgctgaa tcaccgcagc ctttgtcagc aaagaattta acctgctctt
     1081 cttcccagac agcatccagc gcctgcggcg tatcatcgcg cgcttcgtcg gcaatttttt
     1141 ccgccacttt gtcgctgtcg atagcggtat tggccaattt cagcagcaga tcaaacatca
     1201 cggcataacg gctttcacgc tgttgcagac gcgcgctcag caatgccaga atcggcgcga
     1261 tatctttcaa accttccacg gcttcgcttt gcggcagctg cgacaggtac tccagataca
     1321 gcggcagatg gtccggtaat tcgcggctgt ttaactgcaa accgtgctgc tcgtactgcg
     1381 ccagcaggtc caccatcgcc tggccgcggt cacgggattc accgtgaaca tgttcaaata
     1441 gcaacagtga cgtggcgcgg ccacggtcga acagttcgct gtactgggcc tgggcatcga
     1501 gcggatccat cgccgttaaa tcgcgcagga aaatgcccag cgcatgggca tcttctttcg
     1561 acagattttt cgacgcagca atcgcctcaa acatctcttg ttgatgctgc cataaggcag
     1621 catccggata ttcaaggaga cgcgatacaa tcacgagttc gatcatggat gcggctccgt
     1681 tttgctggtc acatcgatgg catcgatacg acggctgttg aacagattga atttggtatc
     1741 tgaaccgtgg cagccatcac caaaggtaaa gccgcagcca tttttctccg ggaaggcttc
     1801 ccgtgccagt tcacgatgac tactcggcac cacaaagcga tcttcgtagt tagcaatcgc
     1861 cagataacgg tacatctcct gtgcctgggc ttcggtcaga ccgacctctt ccagcgcacg
     1921 ggtatcaact ttaccgtcaa cggtttcagc acgtttgtaa tgacgcatcg ccagcatacg
     1981 tttcagtgcg cgcagtaccg gtttggtatc accggcggtc agcagattcg ccagatactg
     2041 taccggaata cgcaagcttt cgacgtctgg cagaatgccg ttgctaccca gctcacccgc
     2101 gtctgctgca gactgaatcg gagacagagg cggcacgtac cagaccatcg gcagtgtgcg
     2161 atattctgga tgcagcggca gcgccagttt ccattccatt gccattttat aaaccggcga
     2221 ctgctgtgcg gcttcaataa cgctcagcgg aataccgtct ttaatcgcct gctcgatgac
     2281 tttcggatcg ttcggatcga ggaacacctc cagctgacgc tggtaaagat ctttctcgtt
     2341 ctcggtgctg gctgcacgtt caatcgcgtc ggcatcgtac agcagcacgc caagataacg
     2401 gatacgaccg acacaggttt ctgagcacac ggtcggctga cccgcttcaa tacgcggata
     2461 gcagaagatg cacttctcag acttaccgct cttccagttg aagtagattt ttttgtacgg
     2521 gcatccggtg atgcacatac gccagccacg gcatttatcc tggtcgatca ggacgatgcc
     2581 atcttcttca cgcttgtaaa tcgcaccgct cgggcaggtc gccacacatg ccgggttcag
     2641 gcagtgttcg cacaggcgcg gcaaatacat cataaaggtg ttttcgaact ggctatacat
     2701 cgccttctgg atgttgtcga agttcttgtc tttcgccagt ttgtcaaact caccacccag
     2761 gtcatcttcc cagttcggcc ctttttcgat tttcgccatc cgttccccgg taatcagcga
     2821 acgcggacgg gcaatcggct gcgatttgct gccttccggc gcggtatgca ggttctgata
     2881 atcaaaatcg aacggctcgt aataatcgtc gatccccggc agatgcgggt tagcgaagat
     2941 tttacccagc agcatggcgc ggttacccat gcgcggctgc agtttgccat tgattttacg
     3001 gatccagccg cctttgtatt tttcctggtt ttcccagtca gtcgggaagc cctgacccgg
     3061 cttggtttcc acgttgttga accacgcgta ttccacgcct tcacggctgg tccagacgtt
     3121 tttacaggta actgaacagg tgtggcagcc gatgcactta tcgagattca gcaccatgcc
     3181 gacttgtgaa cgaattttca ttttacgctc tcctgtacct ggtcattgcc ttcgccatct
     3241 aaccagtcaa tgttcttcat cttacgcact acaacaaact catcgcggtt agaacctacg
     3301 gtgccgtagt agttaaagcc gtatgccaga tgggcatagc cgccgatcat atgcgtcggt
     3361 ttcggcgtga tacgggtgac cgagttatgg ataccaccac gctgttgggt aatttccgaa
     3421 ccaggcaggt taacgatacg ttcctgcgcg tggtacatca tggtcatccc tgccggaaca
     3481 cgctggctga caaccgcacg ggcagtcaga gcaccgttgc tgttgaatac ttcaatccag
     3541 tcgttatcgg cgatacccag atctttggca tcggcttcac tcaaccagac caccggacca
     3601 ccgcgaccta aagtcagcat cagcaggttg tcgctgtagg tggagtggat accccacttc
     3661 tggtgcggcg tcaggaagtt gagcgctttt tccgggttgc cgttggattt ctggcctatc
     3721 acttctttca ccgaacgggt gtcgatcggc ggacgataaa ccagcaggct ttcaccgaaa
     3781 tcacgcatcc actggtgatc ctgatacagt tgctgacgac cagagagcgt acgccatggg
     3841 atcagctcgt gaacgttggt gtaaccggcg ttgtaagaaa cgtgttcatc ttccagacca
     3901 gaccaggtcg ggctggagat aattttgcgc ggctgtgcct gaatatcgcg gaagcggatc
     3961 ttctcgtctt ctttattcag cgccagatgc gtatggtcac gaccggtaaa ttcgctcagg
     4021 gcagcccagg ctttcacggc tacctgaccg ttggtttccg gtgccagtgt caggatcatc
     4081 tctgccgcat caattgcggt gttcagcatc ggctggcctt tcgccggacc ttccgctttg
     4141 gtgtagttga gcttacgcag cagatccatc tcgctctggg tgttccaggc aatcccttta
     4201 ccgccgttac cgattttctc catcagcggg ccgatagaag taaagcgttc gtaagttgcc
     4261 ggataatcgc gctctacgac cataatgtgc ggtgcagttt tgcctgggat caggtcgcat
     4321 tcgccttttt tccagtcttt cacatccagc ggctgcgcca gttcagcggc agagtcatgc
     4381 tggataggca gcgtgacgat gtcggtttct ttacccagat ggccgacgca cacttcggag
     4441 aatttcttcg cgatggcttt gtagatttcc cagtcgcttt tcgcttccca ggccggatcg
     4501 accgccgcag acagcgggtg aataaacgga tgcatatccg aagtattcat gtcgtctttt
     4561 tcgtaccagg tcgccgtcgg caaaatgatg tcggaataga gacaggtgct cgacagacgg
     4621 aagtccagcg taaccaccag atccagcttg ccttccagac cattgtcctg ccagtccact
     4681 tcttccggct tcacgccgcc ctgttgcccc agatctttac cctggatacc gtgctccgtc
     4741 cccagcaggt acttgagcat aaactcatga cctttaccgg aagaaccgag caggttagaa
     4801 cgccagatga acaggttgcg cgggtggttt ttaccgtttt ctggttgttc tgccgcaaaa
     4861 cggatggaac cctctttcag ggatttcacc gtatagtcca ccggattcat cccggctttt
     4921 tccgcttcgc cagcgatagt cagcgggtta gtgcctaact gcggtgcaga cggcagccag
     4981 cccatgcgtt ccgcacggac gttaaagtcg atcaagtgtc cggtatagcg ggatttgtcc
     5041 gccatcggtg acagcaactc ttccgccgtg acggtttcat aacgccactg gctggagtgg
     5101 ttatagaaat aagaagtgct gttcatgtga cgcgccggac gctgccagtc aagggcaaac
     5161 gccagcggct gccagccggt ttgcggacgc agtttttcct gacctacata gtgcgcccag
     5221 ccgcccccgc tctgaccgac acagccgcag aaaatcagca tgttgatcag accacgatag
     5281 ttcatatcga ggtgatacca gtggttcagc cccgcaccga cgataatcat cgaacgaccg
     5341 tgcgttttat cagcgttatc ggcaaattca cgggcgatgc gaataatctg gctgcgagaa
     5401 acgccggtaa tctgctcggc ccaggccggg gtataagctt tcacatcgtc atagctggtt
     5461 gcacagttaa cgtcgttcag gccacgttcc agaccgtagt ttgccagcgt cagatcataa
     5521 acggtggtca ccagggcggt gctgccatca gccagttgca ggcgtttcac cggcagtttg
     5581 tgcagcagca cgttttccag ttccactttg ttgaagtgtt ccgtgccgtc gccaccaaag
     5641 tacgggaagc ctacctcagc gatctcatcc tggctaccca gcaggctcag ttgcagctcg
     5701 gtttcttcgc cagttttgcc gtcgcgctgc tcaagattcc atttgccctt ctcgccccag
     5761 cggaagccaa tagaaccgtt cggcgcaacc atttcgccat tggtattaaa ggcgacagtt
     5821 ttccattccg gattgttttc ctggcccagc gcatcaacca gatcagcagc gcgcaacata
     5881 cgacctgcag cgtagtaacc gtcgcgttct tccagcatca ccagcatcgg catgtcggtg
     5941 tagcgacgca catagtcggt gaaatactgg cttgggttgt cgaggtggaa ttcacgcagc
     6001 attacgtggc ccatcgccag cgccattgcc gcatcggtgc cctgtttcgg tgccagccac
     6061 agatcgcaca gtttggcgat ttcagcgtag tctggtgtga cggcaacagt tttggtgcct
     6121 ttgtaacgca cttcagtaaa gaagtgagca tccggggtac gcgtctgcgg cacgtttgaa
     6181 ccccaggcga tgatgtagct ggagttatac cagtcagcag attccggtac gtcagtttgc
     6241 tcgccccagg tttgcggaga cgcaggaggc aggtcgcagt accagtcgta gaagcttaag
     6301 caagtaccgc caatcagcga gagatagcgt gcacccgatg cgtaagaaac catcgacatt
     6361 gccggaattg gcgagaaacc agcaacacgg tccgggccgt agtttttgat ggtgtaaacg
     6421 ttagatgcgg cgatcagttc gttcacctcc tgccaggaag aacgaacaaa tccaccgcgt
     6481 ccacgcgcct gcttaaagct tttcgcttta tcggcgtctt caatgataga agcccatgcc
     6541 tcaaccggat cgctatgcag cgccttcgct tcacgccaca ttttcatcag gcgtttgcgc
     6601 atcatcgggt atttcaggcg gttggcactg taaagatacc aggagtagct ggcaccgcgc
     6661 gggcagccgc gaggttcatg gtttggcaga tccggacggg tacgcggata gtcagtctgc
     6721 tgggtttccc aggtgaccag accgtttttg acgtagattt tccagctgca ggagccggtg
     6781 cagtttaccc cgtgggtaga gcggacgatt ttgtcatgct gccaacgctg gcgatatcca
     6841 tcctcccagt cacggttggt attgagaagc tggccatgcc catcggcaaa ggtttcaccc
     6901 ttctgtttga agtagcgaaa ccggtccagg aatttactca tcggttttct cctgtgggag
     6961 cctgtcggcc ctctgataaa tcgacattgc taatgtttaa gggcaaggta acgctctgaa
     7021 acagcgtggg aattgataac gatcaagagt gatggggatg aaaaataaag taaattccta
     7081 agatacccct ttaggataat taacagccac cacataacat actgtaaatt aagacgtttt
     7141 atacctgtag ttatttaaac tattaagatt tttatgcaga tgggtattaa ggagtattcc
     7201 ccatgggtaa catattaatg gctcttaatg aaatccggcg aaataaacca tataacatta
     7261 gtatgttgta actaaataac gcataaaaaa agcgcgacat catgccgcgc aaaggataat
     7321 caaatatttt actctatata attcggttta caggaaggcg gcaagtgaat gaatcccagg
     7381 agcttactaa agtaagtgac tggggtgaat gaacgcagcc gcagcacatg taacccgaag
     7441 tatgacgagt aacggtttat tttttagaat gccgaccata taccgcccag gtaatcacta
     7501 cgcaggcgat atagaaaatc aaaaatacct tcattgcgcc gactggcgaa cccgttaatg
     7561 ccagcgagct accaaacgct ttcgggataa agaagccacc aatcgcgcca atcgcagaga
     7621 tgaaacccag cgccgccgcc gtgtcggttg ccgcttcacg catcgcacgt tcgtcagaac
     7681 ccccttctgc tttcacgcga tccattgtca gtttacggaa gatcactgaa atcatctgga
     7741 aagtggaacc actacccagc ccagctgtca ggaacagcgc caggaagacc gcgaagaacg
     7801 ccatgaagct tccgccctgc ccgtcagtcg gtaaggtcag gaacagcagg ccgctgaaaa
     7861 tcgccatcag aataaagttc accagcgtga cacgagttcc gcccagacgg tcagataatg
     7921 caccacctgc agaacgcgcc agcgcaccaa taaacggccc gaagaaagcg tattgcagaa
     7981 tctgaacatc cgggaactgc gtttttgaca gcattgcaaa gcccgcggag aagccgatga
     8041 aggagccgaa ggttgccaga tacagcaggc tcataatcca cagatgaccc cgtttgagta
     8101 ccggcaactg ctccttgatg gaggctttcg aggtagcaag atcgttcatg ccaaaccacg
     8161 ccgcaatggt gaagatggca aggaacggca cccatatcca ggacgcattc gccagataca
     8221 gctcagtccc atccggctgt ttgacgccct ggctaccaaa tactgcgaaa atcgacagtg
     8281 ataccaccag cggagcaacc aactgcatga cgctgacgcc catgtttccc agaccaccat
     8341 tcagacccag cgcgccaccc tgcttctgtt tcggaaagaa gaagctgatg tttgccatac
     8401 tggatgcgaa gttcgcgcca gcaaagccgc atagcagaga gatgatgatg aagacgctat
     8461 aaggcgtgga ggtatcctgc acggcaaaac ccagccagac gcaaggaata atcagaatac
     8521 cggtgctgaa cgccgtccag cgacgaccac cgaagatagg aaccataaag gagtatggaa
     8581 cacgtaataa cgcgccagaa accgaaggca gcgcagtcaa cataaatagc tgatcggtcg
     8641 taaaattaaa accgactttc ggtaagttca ccgcaacagc gctgaacaac atccatacgc
     8701 aaaacgccag cagcagacag ggaacggaaa tccacaggtt gcggctggca atacgttgac
     8761 cgcgttgttg ccagaacgca ggatcttccg gtcgccaatc tgtaatgaca gctccagtag
     8821 ccctttcggg ggcggatgag tgactcatag acacctctga tactcgtttc gatgtctgcc
     8881 accttagtgt ctggagctaa aggcaatttg atgtaaatca aacgataaga taactttatc
     8941 tatcattgat atttatcatt acccatagtg agtacagtga tttcataaaa attatgagat
     9001 ttttcacggt gctgtaaaat ccctaccctt accgatgtaa agcgactaac cacacggcaa
     9061 ataaggagta actctttccg ggtatgggta tacttcagcc aatagccgag aatactgcca
     9121 ttccagaatg tatcgtcaca ttcattaagg ttattgctca tttaaagcct gaaggaagag
     9181 gtttacatgc ttaaacgttg tctctctccg ctcaccctgg ttaatcaggt tgcgcttatt
     9241 gtgttgcttt ctactgctat tggactggca gggatggcgg tttctggctg gctggtgcaa
     9301 ggcgttcagg gcagcgccca tgcgatcaac aaagcgggat cgctgcgcat gcaaagttac
     9361 cgtctgttgg cagcagtgcc attaagcgag aaagacaagc ccttaattaa agagatggaa
     9421 caaacggcat ttagcgccga gttgactcga gcagcagaac gagacggaca actggcgcaa
     9481 ttacagggtt tacaagatta ctggcgtaat gaactgatcc ctgcactgat gcgtgcacaa
     9541 aaccgcgaaa cggtgtcagc ggatgtcagc cagtttgttg ccgggcttga tcagctggta
     9601 tctggttttg accgcaccac ggaaatgcgc attgagacag tggtactggt ccatcgggta
     9661 atggcggtat ttatggcact tttactggtg ttcactatta tctggttgcg ggcgcgactg
     9721 ctacaaccgt ggcggcaact gctggcaatg gcgagtgccg tcagtcatcg cgattttacc
     9781 caacgcgcga acatcagcgg gcgcaacgaa atggcgatgc ttggaactgc attgaacaat
     9841 atgtctgcag aactggccga aagttatgcc gtacttgagc agcgggttca ggagaaaacc
     9901 gccgggctgg agcataaaaa tcagatcctc tcttttttat ggcaggctaa ccgccgtttg
     9961 cattcccgcg ccccgctgtg tgaacgcctg tcacctgtac tcaacggctt acagaattta
    10021 accctgctac gtgatatcga attgcgggtg tatgacactg atgatgaaga gaatcatcag
    10081 gagtttacct gccagccaga tatgacttgt gatgataaag gctgccagct ctgcccgcgc
    10141 ggcgtattac ccgttggcga tcgcggcaca accctgaagt ggcggctggc tgactcacat
    10201 acgcagtacg gtattttgct ggcgacccta ccgcaggggc gtcatcttag ccatgatcaa
    10261 caacaactgg tggataccct ggttgaacaa ctcaccgcca cgctggcgct ggatcggcat
    10321 caggaacgtc agcaacagtt gatcgtgatg gaagagcgtg ccaccattgc gcgcgaactg
    10381 catgattcta ttgcccaatc tctctcttgc atgaagatgc aggtgagttg tttacagatg
    10441 cagggcgatg cgctgccaga aagcagccgc gaactgttaa gtcagatccg taacgaactg
    10501 aatgcatcct gggcgcagtt gcgtgaattg ctcaccacat tccgcttgca gctcaccgag
    10561 cctggattac gtccggcgct ggaagcgagt tgcgaagagt acagcgccaa atttggcttc
    10621 ccggtgaagc tggattatca attgccgcct cgtctggtgc cttcacatca ggcaatccac
    10681 ttgttgcaaa ttgcccgtga ggcattaagt aacgccctca aacattcgca agcgagtgag
    10741 gtcgtggtga cggtggcgca aaacgataat caggtcaaac tgaccgtcca ggataacggc
    10801 tgcggcgtgc ctgaaaatgc catccgcagc aatcactacg gcatgataat aatgcgcgac
    10861 cgtgcgcaaa gtttacgagg cgattgccgc gtccgccgtc gtgaatcagg tggcaccgaa
    10921 gtggtggtca cctttattcc cgaaaaaact ttcacagacg tccaaggaga tacccatgag
    10981 taatcaggaa ccggctacta tcctgctgat tgacgatcac ccgatgctgc gaactggcgt
    11041 aaaacagctt atcagtatgg caccagatat caccgtggtt ggcgaagcga gtaatggcga
    11101 acagggtatt gaactggcgg agtctcttga tcccgatctg atcctgttag atctcaatat
    11161 gcccggcatg aacggtctgg aaacgctgga taaactgcgc gaaaagtccc tctcagggcg
    11221 cattgtggta ttcagcgtct ctaaccatga agaagatgtg gtcaccgcac tgaaacgcgg
    11281 cgcggatggc tatctgttaa aagatatgga accggaagat ctgctgaaag cattgcatca
    11341 ggcagctgct ggcgaaatgg tattaagcga agcattaacg cctgttctgg ccgccagctt
    11401 gcgcgctaac cgtgccacta ctgagcgcga tgttaaccag ttaaccccac gcgagcgcga
    11461 tattctcaag ctgattgccc agggtttgcc gaacaagatg attgcccgcc gcctggatat
    11521 caccgaaagc acagtaaaag tgcacgtcaa gcacatgctg aagaaaatga agctcaagtc
    11581 tcgcgtggaa gcagcggtat gggtgcatca ggagcgcatt ttctgattac ggttcccagc
    11641 gcagttcgtc gtttgacaat gcgtcgaacg gttcgacaag cgttagcgtg atctcattgg
    11701 aggagacacg ctgcccctgg ttatcttcca ccaccaccga caatcgccag tgattgctcg
    11761 ccccttcccc gttctgccag tcaggcatga tcagcgtcca gccctccgcg ctgttggctt
    11821 gtgcgcctgg cgtcaaactt aatatctgcg tatcgccctg ccaaatcagt tgccgaatac
    11881 cgtaacgact gcggatttgt aatttcagcg gcactgtttc gccaggtttt agatcccacg
    11941 gcggtgtcgc cagaaacacc gttaacgttt ttcgctgtcg gtactcaaga gtcggtagat
    12001 tatttcgctg cggattatca tagcgactac cacgtaacga ctgactttcg gcaacctcgc
    12061 ccgcagaaag ttgttttttg agcggtacac caaagcggta attaagattc agcccgaggt
    12121 tattttgatt ctcgccactt tcaccctgtt tatgctgggc cgtcacagtg actaatggca
    12181 caggggtgta atttaatccc agactcaacg cgacgggatt gtgataaccc gtaccagagt
    12241 taaacaaatc aacacgatca ccaaaatact gttctacgct gacactggta ttgaggtgtt
    12301 gatagaacgg catgcgcatc cgggctgtca ggtcgtaccc gcgcgccatc cgttgttcct
    12361 gcgtggctgt ctgttcatgc catgcagcaa atggctgata aaagtttgcc gatagtcgta
    12421 aatattcgcc ccacgcttcg gcaccaaagc ccgctcgctg aagattttcg tccagcaagt
    12481 tgtcataaaa agtgttataa cccaccagcc agttgccgcg cgcccagcgt tgcccaacgc
    12541 ccacattgct caccaaccca tcatcctgct gagtaagacc aagctggctc caggtgagat
    12601 aacgatcatt atcttgtaac ggcacaaacc agcttccacg actgccggta aaatgacctt
    12661 cgttatccac tttgacatca acactggcat ttccccacgg tgatagccag gactctacgt
    12721 gctgattaac ctgttgacta agcgcgtcgc ggacttttcc caatgcgaaa gcttttgcct
    12781 gctcgccagt atccagcccg ttatcattca tactggtttc gccgaaatct ttcacaattt
    12841 cagcaaagtg tttttcccca tcatgatttt caggtgccat gcctaaatcc ggcagaccat
    12901 cattgttatt atcaaaggga tttgccgctt tttgctcgaa ggtagattgt gcgttagctg
    12961 taccgcctgc cgccagcaag agtaaataaa acgaaataat gcgaggaacg aaacggctca
    13021 aaacgtcggt aacttccggt aaaaaatgaa aaaccatagc ctttaagaat aacgattact
    13081 ggcaactttt gtcgtatttt ctcgcgcttt caggaaaatc cttaaaaatg tgaccgattg
    13141 gtcagacgct gcagaagcag taagatagaa gatgagtgtc taattccctg aatacaggag
    13201 taaataatgc aaaaaatcgt gatcgttgcc aatggcgcac cttacgggag cgaatccttg
    13261 tttaacagct tgcggctggc cattgcgtta cgagagcagg agagcaatct ggatctgcgt
    13321 ctgttcctca tgtctgatgc ggtcacagcc gggttgcgcg ggcaaaaacc aggggaaggc
    13381 tacaacattc agcaaatgct ggagatcctt accgctcaga atgtaccggt gaaattgtgc
    13441 aaaacctgta ccgacgggcg cgggattagc acacttcctc ttattgatgg ggtggaaatc
    13501 ggtactcttg tggaactggc gcaatggacg ctgtcagccg ataaagtgct cacattttaa
    13561 tgtttgaggg ggcgtctgac gccccactat gagggaagtt tactcaggcg aatcccggcc
    13621 gtaacacacc atccggataa ttctccgcca gcagtttttt taccgatacc agcaaatcat
    13681 tcagcccatc gtcctgcatt cccagtttga taagctcctg ttccagtgaa aacaggtatt
    13741 gtgcgttggt tcccagcgga ccgctcgccg ccgcaatcaa cggcgcgatg acctgagcgc
    13801 gagtgtcaga ttcatattct ggatgtcgcg ggtccataat aaacacaatg gcgtttactg
    13861 tgcatccatc atcaagatca agctgacacc aggttggcag ataacagccg gtaatcatct
    13921 cgcgcttcca caacagggtt agttcctgct ccagcgtctc ttctggcagt cgataggcga
    13981 cgcctgtagt gcgtccgccc tctttcagtg caagcatccg tcccggctgg tgcgcagtcc
    14041 cgcgcccggc ggtcaggcgc aggcaaaacg cgcgatgcca tccaaccagt gtaccggtgc
    14101 acgactcggt aaactccagt gccggattcc acatcaacga tccataaccg aagatccaca
    14161 ccggtccctc atcaggtcga caagccagcg tcgccgccag cgaagccgcc cgttgctctg
    14221 ctgaccataa gagtgattct tcaatggcac caaatgccgt tttacaatcg gcattcatca
    14281 agaaatcacg cgttatcacc ttacacctcc gccactgcat acttccctgc gacaactttt
    14341 tggtgtcttc cggacatttt tactgctcat tttgaaacca ctaacgggac aatatgcaat
    14401 ccacggacca cttgcaaggc aacaccgcac gaatagcttt aactaagcta ccggttttac
    14461 gattttttat gccatttatc atcatcccct ttggcatatt catgcttcac agcagcccag
    14521 gccactttat gcgcggtttc ttcgcgactg gcgtcatcac gccgatcctc tttatcttta
    14581 tattgatccc acgcgctgtt gaacgcttct ttatagatat cctgggcatg agacggtaga
    14641 acgtgcttta cgctttccgg cagatcgctt ttcgttttat acggcatcgt tgactccttt
    14701 tacaaagttg aacattaagt gtggtcaaca atatctactc ttgccaaaac ataagaattt
    14761 tcatgtcaga ctgttcttaa gaaacgctaa aacaaacatt gtctaaacgt aatacttatt
    14821 tttatgtccc aatgagtaaa aaatcgacag tggggtaaaa attcgtaaaa tgttaccgat
    14881 atccagacac tttctgttat ttttcctacc tattttttac attttcgata aattcatctg
    14941 caaagggata accataatgt caaatgctca agaggcggta aaaacccgcc acaaggagac
    15001 ttcgcttatt ttcccggttc tggcgctggt agtgctgttc ctgtggggaa gcagccagac
    15061 actaccagtg gtcattgcca tcaatcttct tgcgcttatt ggtattttaa gtagcgcctt
    15121 tagtgttgtc cgtcatgcgg acgtattagc ccatcgcctg ggagaacctt acggttcgct
    15181 tattcttagc ctttcagtgg ttattcttga agtcagtttg atttcagctt taatggcaac
    15241 cggcgacgcc gcgccaacgc taatgcgtga tacgctctat tcaatcatta tgattgttac
    15301 cggtgggctg gttggctttt cattattatt gggcggtcgt aagtttgcca cccaatatat
    15361 gaatctgttt ggtatcaagc agtatttaat tgcgctgttc cccctggcga taatcgtact
    15421 ggtatttcca atggctctgc ctgcggcgaa tttttcaacc ggtcaggcgt tactggtagc
    15481 attaatttct gcggcaatgt atggcgtatt tttgctgatc cagaccaaaa cgcatcaaag
    15541 tttgtttgtc tacgagcacg aagatgacag tgatgacgac gacccgcatc acggtaaacc
    15601 gtctgcccat agcagcgtgt ggcatgctat ctggttgatt atccatctga ttgccgttat
    15661 tgcagtcacc aaaatgaatg ccagcccgct ggagacattg ctcgacagca tgaatgcccc
    15721 tgtcgccttt actggcttcc tggtggctct gttgattctg tcgccggaag gtttaggtgc
    15781 gttaaaagca gtgttgaaca accaggtcca gcgcgcgatg aatctgttct ttggttcagt
    15841 gttagcaacc atttcgctta ccgtacctgt cgtcacgcta attgccttta tgacgggtaa
    15901 cgaattacag tttgcacttg gtgcgccaga aatggtggtg atggtggcct ctttagtgct
    15961 gtgccatatc tccttctcca ccggacgtac taacgtgctc aatggcgcag cgcatctggc
    16021 actgtttgcc gcctatttga tgacgatatt tgcctgacag ttatatctcc tccgatgacg
    16081 gaaatcgggg gagataagat aaattcctgc ttattgtatt tcttcatact acctggacag
    16141 ctcgttaatc agaattgcga gcctgtacca caacctgcac gaaaccactg caacagctgt
    16201 tgaaaagccg gaatgtactc tttattgcaa acatttcagg gatgaagtga agatgaattg
    16261 agaagtagta tccacaccaa gccggaatta tccggtgagg cgcaatgttg cgggggcttt
    16321 atccctggtg gcattggttg ctggaaagag aaaacccccg cacgttgaga ggtaaaacct
    16381 gacaacagaa cgggggctaa tcttgaacca gaacaattca agaatagccg caaaatgttg
    16441 tcattacaac aaggcggcta tatgacgctc gcgcagtttg ccatgatttt ctggcacgac
    16501 ctggcagcac cgatcctggc gggaattact accgcagcga ttgtcggctg gtggcgtaac
    16561 cggaagtaag gtgaaaacgg gcgtcaggct gccgtgatgg caatatgcgc ccgaaaccgg
    16621 ccgcaggggt aacagcctgc ggcctttttt gtatctggca cccatgaaaa aagtcttaac
    16681 gcagaacgct aagactttat ttttcaagca aaaaagatta cttactggta tccagttctt
    16741 cgaaaccttt caccagatca tcaatcgctt tcatctgctt gaggaacggt tccagtttag
    16801 ccagcggcag cgcggatgga ccatcacatt tcgcatgttc cggatccgga tgcgcttcaa
    16861 taaacagccc cgccagacct accgccatac cggctcgtgc cagctcagcc acctgagcac
    16921 gacgaccacc ggaagctgcg ccaaacggat cgcggcattg cagtgcgtgg gtcacgtcga
    16981 aaatcactgg cgagttacca gacactttct tcataatgct gaagcccagc atatcgacaa
    17041 ccaggttgtc atagccgaag ttagcaccac gatcgcaaag aatcactttt tcgttgccgc
    17101 cttctttgaa tttatcaacg atattaccca tctgtcccgg gctgacaaac tgtggtttct
    17161 tgacgttaat taccgcaccg gttttcgcca tggcttcaac caggtcagtc tggcgagcaa
    17221 gaaacgccgg caactgaatc acatccacga catcagcaac gggctgtgcc tgacttggtt
    17281 cgtgaacgtc ggtgataatt ttcacgccaa aagtctgctt caactcctgg aagattttca
    17341 tcccttcttc caggcccggt ccacgataag agtggatgga ggagcggttg gctttgtcaa
    17401 aagaggcttt gaacacgtaa gggataccca gtttctgggt cacggttacg tagtgctcgc
    17461 aaatgcgcat cgccagatcg cgagattcca gcacgttcat accaccaaac agtacgaacg
    17521 gcaggtcatt tgctacgttg atgtcgccaa tgctaaccac tttttgtttc ataggatcgc
    17581 cttaatcttg agtaaaatgt cgattaatta atgcagcaca atatgtttat gcgcgatgtt
    17641 atttatttgc gcacggatca tttcgctgat cgggtcttcc ggacactgtt caacgaaata
    17701 acttaaatcg ttcaacgcaa cgtgttcgca atccagttgc gcataaatca acccgcgatc
    17761 gcgaatttca tagggatctt ccgggttgaa ttgtaataaa gcttcgctgg tgcgtaacgc
    17821 cagctccatc tgattttctt ccatcaacga ggctttgagt gtatccagca atttgcggat
    17881 tacctcaatg ttatcagctt catcaaggtc ttcataaaac agttccgccg acgggctgat
    17941 atttcccttt aaccatacgt ccagcatatg ttcgcttaac gattcaccgt taaaaggatt
    18001 aatcagccaa atttcgccat ccggacattc aatgcgcaat atcagctgcg tagggaaaat
    18061 caccggcagc agcggcaaat cgagacgatt cgcgacccat aataaaaccg cacctaatga
    18121 taccgcactg ccctgtcgat tctttaacac ctggtccagc cataatgcat cggaaagacg
    18181 ataaacgccg cgtgaggctt taaatcccca gtcgccgtaa aacagcgcga tcagtttttc
    18241 cagctgctct tctaaaggca gaagctggct gatttcttcc ttcgccagac taacgagacg
    18301 ctccagttcg tcgtaaacat cttgcgaggg aaaatcgcgg cggattgctt cgcaagccag
    18361 gatcatgcct tcgcacaatg gcgctttatt aaattcgaaa tcagctaacg atctcatgac
    18421 ttaccccagt aacggtactt ttgtggtggc gagtttaatg atgatgtaca gcaccaccaa
    18481 cgccagcggg aaggcaataa tgcgcgcctg ctgactgtgc atacgacgat aatcgagtgc
    18541 aataaaaccc aaaacgatat aaatgataac tccaaacagc ttttcagtca gccactgtgc
    18601 ctgcccggaa aatggcagga tgtgcgcttt agccatcaac gcaatgccgc ttaacaataa
    18661 caccgtatcg acaaccggcg gcacaatgcg cgtccagcga gcaaatgcct gtggatgctt
    18721 ctgataacgt agccagaaac gtaaggttaa tagcccaaca gaaagcgcga tactaataag
    18781 atgaacacta agcagtgtag aaaaacttgt cattgataat agcggccgag cgttacgcgc
    18841 tcgttatcac catagtcacg gcaggtttcg acgtcatgat accccgcgag gataaatgct
    18901 tgtcgcaccg cttcgccctg ctgccagcca tgttccagaa gcagaaagcc gccggatacc
    18961 agcgcgttac gcgactgttc gatgatatgc acgatgtctg ccattccact gtctgccgca
    19021 accagcgcag tgagcggctc aaagcggaca tcgccttgtt gaagatgtgg gtcctgctcg
    19081 tcaatatacg gcggattgct gacaatcatc gtaaactgct gcccggccag cgcactaaac
    19141 cagtcgcttt gcagaatgcg gatatttttg atcgccagat gctgggcatt acgttgtgcc
    19201 agagcgacag catcgggcat acgatctaca gcggtaattt cgcagtccgg gcgctcgcta
    19261 gccagcgcaa gcgcaatcgc cccggtaccc gtcccgagat cgagaatacg gcaaggttgt
    19321 tcaggcaacc gcgccagtgc ctgctccacc agacactccg tatccgggcg cggaattaag
    19381 gtcgctggcg aaacaaataa cggcaacgac cagaattctc gcaccccggt taaatgagca
    19441 atgggttcac catcgcgacg acgtgtcagt agcgcatcaa gttgctgaca ttgttcgtca
    19501 gtcagctgcg tttcaccaaa ggcgaggata aaagtacgcc ctttgccggt aacatgttcc
    19561 agcaggattt cagcatcacg ccgcgggctt tcgctcgcct gaagttggct tattgcttca
    19621 cgtaaccagt gttgatattc cattattcct gctcggacaa cgccgccagt tggtcggcct
    19681 gatgttcctg gataatcggt tcaatcagca tatccagctt accttccatc acttcatcca
    19741 ggcggtagag cgtcaggttg atgcggtgat cggtaacgcg cccctgtggg aagttgtaag
    19801 tacggttacg gtcgctgcga tcgccactcc ccagcaggtt acgacgggta gacgcttcgg
    19861 cctgttgacg ttttgccatt tcagcagcgt ggatgcgagc accgagtaca gaaagtgctt
    19921 tagctttgtt tttatgttgt gaacgttcgt cctgacattc aacaacaatc ccggtcggca
    19981 agtgagtaat acgaattgcc gaatcggtgg tgttaacgtg ctgaccaccc gcccctgacg
    20041 agcggaaagt atcaatgcgt aaatctgctg ggttgatgtc cggcagttct gcgtcaggca
    20101 gctctggcat taccgcaacg gtacaagcag aagtatgaat acgcccctgc gattccgtag
    20161 caggaacacg ctgcacgcga tgaccgccag attcaaattt cagacgacca tacacaccat
    20221 caccgctaat tttggcgatg atctctttat aaccaccatg ttcaccctcg ctggcgctca
    20281 tgatttctac ccgccagcgg cgggcttcgg cataacggct gtacatacgg aacagatcgc
    20341 ctgcgaacag cgccgcttcg tcgccgccgg ttccggcacg gacttcgagg aaggcgttac
    20401 gttcgtcatc aggatctttt ggtagtaaca gaacctgtaa ttgctgttcc agttgctcgc
    20461 ttttttcttt agcttcgcgc agttcatcct gcgccatctc acgcatttca ggatcatcga
    20521 gcatcatctg tgcggtttcg atatcttcct gaacctgttg ccagtcggta aaacagcgcg
    20581 aaacatcact taactgcgca tattcgcgtg ataatgcgcg aaaacgttcc tggtcggcga
    20641 tagtttgcgc gtcacccagc aacgcctgaa cttcttcatg gcgttcatgc agggcttcca
    20701 gtttggcaac gatagaaggc ttcataggcg taaatgcacc ctgtaaaaaa agaaaatgat
    20761 gtactgctac tccagcccga ggctgtcgcg cagaatattc aggcgttcgt tatccccgtc
    20821 acgggcggcc tgttgaagtg atttcgttgg cgcatggatc aagcggttag tcagtttcca
    20881 tgccagatcc tgcataatgg cttgcgcgtc gccgccctgc tcaagggccg ctaacgcttt
    20941 ggcggttaac tcatcgcgaa cttgctctgc ctggctgcga tactcgcgaa tggtttcgct
    21001 ggcgctttgt gctcgcagcc acgccataaa ttcgctggtt tcctgagcga caatagtttc
    21061 cgcctcaacc gctgcggctt tacgctgcgc caggttgtgc gaaatgatgc tttgcagatc
    21121 gtccacgcta taaagataag cattcgccag tttgccaact tccggctcaa catcgcgcgg
    21181 aacggcaata tccaccaaca gcattggttg gttgcgacgg ctttttaatg cgcgctccac
    21241 catgcctttc ccgataatcg gtaacgggct ggcggtggaa ctgatgatga tatcggcttc
    21301 gcgcagacgt tcgtcgatct cactcagggc aatcacttcc gcgccaactt catctgccag
    21361 tatttgggca cgttcgcgag tgcggttggc gataatcatc ttctgtactt tatgttcgcg
    21421 cagatgacgc gctaccagct cgatggtttc gcccgcgcct accagcaaca ctgtgaccgt
    21481 agagagcgat tcaaagatct gccgcgccag cgtacaagcc gcaaaagcga cagacacagc
    21541 gctggcaccg atatctgttt cagtgcgaac gcgtttcgct acagagaaag atttctggaa
    21601 catgcgttcc agttcgctgg ccttcatatg gcctttttgc gaatcggcaa acgctttttt
    21661 aacctgaccg aggatctgcg gctccccaag aaccaatgaa tccaggccgc tggcaacacg
    21721 cattaaatgg ctaaccgcgt cgttatcctg atgccagtag aggcttttac gcagatcttc
    21781 ttcattaaga ttgtgataat cgcaaagcca gcggattaac gcctcttgca ggttatcctg
    21841 ctcttcaacg ctaagataaa gttccgtgcg gttgcacgtc gacagcacca cgccgccctg
    21901 caccatcggc tgcgcaagca ggctgtcaag cgcctgatcg agcttatccg gcgaaaacga
    21961 tacacgttct cgcagcgata caggtgccgt tttatggttg ataccgagtg ctaaaagggt
    22021 catgtctgcg ggaaataata ccaacgttga tagggttagt ctgcttgcat catacaggat
    22081 gcgtaagatc aataaaagag agcgccccct tttggagtaa ttgccgaaag ccgttagatt
    22141 ctggcaatta agacaacttg aacatagacg atagcggacg gtaacgctag cattaagggt
    22201 tataactgca acgtatctca aggacttgtc atcactatgc ccctgcccga ttttcgtctt
    22261 atccgcctgc taccgctggc tgctcttgtg ctcactgcct gttccgttac cacgcccaaa
    22321 ggtcctggca aaagcccgga ttcgccacaa tggcgtcagc atcagcaaga cgtgcgcaat
    22381 cttaatcagt atcagactcg cggcgcgttc gcttatattt ctgaccaaca aaaagtgtac
    22441 gcccgctttt tctggcagca aaccggccag gatcgctacc gtctgctgct cactaaccca
    22501 ttgggcagca cggaactgga gctgaatgct caaccgggta acgtgcagtt agtcgacaat
    22561 aaaggtcagc gttataccgc cgatgacgcc gaagagatga ttggcaaatt gaccggaatg
    22621 ccaattccgc tcaacagctt gcgccagtgg attttaggtt taccgggtga tgcaaccgac
    22681 tacaaactgg acgaccagta ccgcctgagc gaaattacct acagccagaa tggcaaaaac
    22741 tggaaggttg tttatggtgg ttatgacacc aaaacgcaac ctgcgatgcc agccaatatg
    22801 gaactcaccg acggtggtca acgcatcaag ttaaaaatgg ataactggat agtgaaataa
    22861 tgcggacaca gtggccctct ccggcaaaac ttaatctgtt tttatacatt accggtcagc
    22921 gtgcggatgg ttaccacacg ctgcaaacgc tgtttcagtt tcttgattac ggcgacacca
    22981 tcagcattga gcttcgtgac gatggggata ttcgtctgtt aacgcccgtt gaaggcgtgg
    23041 aacatgaaga taacctgatc gttcgcgcag cgcggttgtt gatgaaaact gcggcagaca
    23101 gcgggcgtct tccgacggga agcggtgcgg atctcagcat tgataagcgt ttgccgatgg
    23161 gcggcggtct cggcggtggt tcatccaatg ccgcgacggt cctggtggca ttaaatcatc
    23221 tctggcaatg cgggctaagc atggatgagc tggcggaaat ggggctgacg ctgggcgcag
    23281 atgttcctgt ctttgttcgg gggcatgccg cgtttgccga aggcgttggt gaaatactaa
    23341 cgccggtgga tccgccagag aagtggtatc tggtggcgca ccctggtgta agtattccga
    23401 ctccggtgat ttttaaagat cctgaactcc cgcgcaatac gccaaaaagg tcaatagaaa
    23461 cgttgctaaa atgtgaattc agcaatgatt gcgaggttat cgcaagaaaa cgttttcgcg
    23521 aggttgatgc ggtgctttcc tggctgttag aatacgcccc gtcgcgcctg actgggacag
    23581 gggcctgtgt ctttgctgaa tttgatacag agtctgaagc ccgccaggtg ctagagcaag
    23641 ccccggaatg gctcaatggc tttgtggcga aaggcgttaa tctttcccca ttgcacagag
    23701 ccatgcttta agccgggcaa gctgagtttc ggtgacaacg tcaccttgtt ccagacgttg
    23761 catcgcgctc tttaatacac cgcctggaaa ggatcatgcc tggcccgcac agttttcggc
    23821 agattctttc caccaatgga cgcatgcctg aggttcttct cgtgcctgat atgaagcttt
    23881 ttgctggtaa cgccaccccg gaactagcac aacgtattgc caaccgcctg tacacttcac
    23941 tcggcgacgc cgctgtaggt cgctttagcg atggcgaagt cagcgtacaa attaatgaaa
    24001 atgtacgcgg tggtgatatt ttcatcatcc agtccacttg tgcccctact aacgacaacc
    24061 tgatggaatt agtcgttatg gttgatgccc tgcgtcgtgc ttccgcaggt cgtatcaccg
    24121 ctgttatccc ctactttggc tatgcgcgcc aggaccgtcg cgtccgttcc gctcgtgtac
    24181 caatcactgc gaaagtggtt gcagacttcc tctccagcgt cggtgttgac cgtgtgctga
    24241 cagtggatct gcacgctgaa cagattcagg gtttcttcga cgttccggtt gataacgtat
    24301 ttggtagccc gatcctgctg gaagacatgc tgcagctgaa tctggataac ccaattgtgg
    24361 tttctccgga catcggcggc gttgtgcgtg cccgcgctat cgctaagctg ctgaacgata
    24421 ccgatatggc aatcatcgac aaacgtcgtc cgcgtgcgaa cgtttcacag gtgatgcata
    24481 tcatcggtga cgttgcaggt cgtgactgcg tactggtcga tgatatgatc gacactggcg
    24541 gtacgctgtg taaagctgct gaagctctga aagaacgtgg tgctaaacgt gtatttgcgt
    24601 acgcgactca cccgatcttc tctggcaacg cggcgaacaa cctgcgtaac tctgtaattg
    24661 atgaagtcgt tgtctgcgat accattccgc tgagcgatga aatcaaatca ctgccgaacg
    24721 tgcgtactct gaccctgtca ggtatgctgg ccgaagcgat tcgtcgtatc agcaacgaag
    24781 aatcgatctc tgccatgttc gaacactaat cgaacccggc tcaaagaccc gctgcggcgg
    24841 gtttttttgt ctgtaatatc catttgtatg acctatgcct ccttcacctg ccatttagtt
    24901 gacagatgat gcgctcacgg atgaaacatt attgtgaaca aaatattttc ctcacatgtg
    24961 atgcctttcc gcgctctgat cgacgcttgc tggaaagaaa aatatactgc cgcacggttt
    25021 acccgtgacc tgattgccgg gataaccgtc gggattattg ctatcccgct ggcgatggcg
    25081 ttggctattg gtagtggtgt ggcaccccag tacggtttat ataccgcagc tgttgcgggg
    25141 attgtcattg ctctgacggg tgggtcacgc tttagcgttt ccggtccgac tgcggcattt
    25201 gtggtaattc tctatcccgt gtcgcaacag tttggactgg caggactgct ggttgcgacc
    25261 ttgctgtcgg ggatcttttt gattctgatg ggtctggcac gctttggtcg cctgattgag
    25321 tatattccgg tttccgtcac cttaggtttc acctcgggta tcgggatcac catcggtacc
    25381 atgcagatta aagattttct cggtctgcaa atggcccatg tcccggaaca ttatctacaa
    25441 aaagtcggcg cattatttat ggcgctgccg accattaatg tgggtgatgc tgccattggc
    25501 attgtgacgc taggtattct tgttttttgg ccgcgtctgg gcattcgttt acccggtcac
    25561 cttccggcct tgctggctgg ttgcgcggtg atggggattg ttaacctgct cggcggacat
    25621 gttgctacca tcggttcgca attccactac gtcctggccg atggttctca gggtaacggt
    25681 attccgcaac tgctgccgca actggtgctg ccgtgggatc tgcctaattc agaattcacg
    25741 ctaacctggg attctattcg cacactgctg cctgcggcat tctcaatggc aatgctcggc
    25801 gcaatcgaat ctctgctctg cgccgtggtg ctggatggta tgaccgggac gaaacacaag
    25861 gcgaacagcg aactggttgg acagggactg gggaatatta tcgctccgtt ctttggtggt
    25921 attaccgcta cagctgccat cgcgcgttct gccgctaacg tccgtgccgg ggcaacgtcc
    25981 cctatctcgg cggtgatcca ctctattctg gttattcttg ccctgctggt actggcaccg
    26041 ctgctctcct ggctgccgct ttccgccatg gcagccctgc tgttgatggt ggcgtggaac
    26101 atgagtgaag cgcacaaagt ggtcgacttg ctgcgtcatg cgccgaaaga tgacatcatc
    26161 gtcatgctgc tgtgcatgtc gctgaccgtg ttgtttgata tggttattgc catcagcgtg
    26221 gggatcgtgc tggcatcgct gctgtttatg cgtcgtatcg cacgtatgac tcgcctggca
    26281 ccggtagtcg tagatgttcc agacgatgtc ctggttctgc gcgttattgg cccgctgttt
    26341 tttgctgctg ctgaaggctt attcacggac ctggagtcac gtcttgaagg caaacggatt
    26401 gtgattctga agtgggatgc cgttccggta cttgatgctg gtggtcttga tgcgttccag
    26461 cgttttgtga agcgtctgcc cgagggatgt gaactgcgcg tgtgcaacgt ggaattccag
    26521 ccactgcgca ctatggctcg cgctggcatt caaccgatcc cgggacgcct ggcgttcttc
    26581 ccgaatcgtc gcgcggcgat ggcggattta taagtacgag attgaccagt cagcgcaaac
    26641 tgactggtca gcaaactgca ttatttgtta gctatgacgg cgattatcgc tacggcggca
    26701 acgtttgtca tagtggcgaa cccaccagta acggtcgcac acctgttcat gcccgccaac
    26761 acgggcaccc gccagccaga gaatggcacc gacgaaaata cttagcactg caccatgtgc
    26821 gaaatactgg ggcatattaa actgtggtaa ctggttgagg attgaatacc ccacgccgac
    26881 caccattacc accagaccca accccatgag cacgttaccg agtaacgaag cgtttttgcg
    26941 tttcatatgt cacctccgga actttctggg ttgtaacagg gaatacccct cttccttatg
    27001 tgtaaagtat agacaacacc cagtgggtta tgtgcgggcg tgatcacaat tacaaccctt
    27061 atttcaacaa aactttacaa ataaacgcct gaacaccttg actttctaat agtccactca
    27121 ggcaattatt acgttatctg catttgatac gcagtttttt gtcaagcggg gccgcaacca
    27181 gtaaactacg cgccagttat gtacacactc aggacaaaaa aacgtgacga ttaaattgat
    27241 tgtcggcctg gcgaaccccg gtgctgaata cgccgcaacg cgacataatg ctggtgcctg
    27301 gttcgttgac ttactggcag agcgtttgcg cgctccgctg cgcgaagagg ctaaattctt
    27361 tggttatact tcgcgagtca ctcttggagg cgaagatgtc cgcctgttag tcccgactac
    27421 atttatgaat ctcagcggca aagccgttgc ggcgatggcc agttttttcc gcattaatcc
    27481 ggacgaaatt ctggtggccc acgacgaact ggatctgcct cctggcgtcg ccaaatttaa
    27541 attgggcggt ggccatggtg gtcacaatgg actgaaagac atcatcagta aattgggtaa
    27601 taaccctaac tttcaccgtt tacgcatcgg aatcggtcat ccgggcgata aaaataaagt
    27661 tgtcggtttt gtgttaggca aaccgcctgt tagtgaacag aagttaattg atgaagccat
    27721 tgacgaagcg gcgcgttgta ctgaaatgtg gtttacagat ggcttgacca aagcaacgaa
    27781 ccgattgcac gcctttaaag cgcaataagt cgttgtctgc ggcatttttg ccgagtgccg
    27841 tgtataatag gcaaagttat ttccatttct gcaatctgtt agcaataaca ggttgattat
    27901 taagatatta aggtgattta aatcatggga ttcaaatgcg gtatcgtcgg tttgcccaac
    27961 gtcgggaaat ctaccctgtt caacgcgctg accaaagccg gtattgaagc ggccaacttt
    28021 ccattctgca ccattgagcc gaacacaggc gtcgtaccaa tgcctgatcc tcgcctggat
    28081 caactggctg aaatcgtaaa accgcagcgt acgcttccca cgaccatgga atttgtcgat
    28141 atcgccggtc tggtaaaagg cgcatcgaaa ggcgaaggtc tgggtaacca gttcctgacc
    28201 aacatccgtg aaaccgaagc gatcggtcac gttgttcgct gctttgaaaa tgacaacatc
    28261 attcacgttt ccggcaaagt taacccggct gacgatattg aagttatcaa caccgaactg
    28321 gcgctggcag acctcgacac ctgcgaacgt gcgattcatc gcgtacagaa gaaagccaaa
    28381 ggtggcgata aagacgcgaa agctgagctg gcggtcctgg aaaaatgcct gccccagttg
    28441 gaaaatgcag gtatgctgcg cgcgctggat ttaagcgctg aagagaaagc ggctattcgt
    28501 tacctgagct tcctgacgct gaaaccaaca atgtacatcg ccaacgtcaa cgaagacggt
    28561 tttgaaaaca acccatatct tgaccaggtg cgtgaaatcg cggcgaaaga aggttctgtt
    28621 gtggttccgg tttgtgctgc tgttgaagca gacattgccg aactggacga cgaagaacgt
    28681 gacgagttta tgcaggagct tgggctggaa gagccgggcc tgaaccgtgt gatccgtgcc
    28741 ggttataaac tgctgaacct gcaaacttac ttcaccgctg gggtgaaaga agtgcgtgca
    28801 tggaccattc cggttggagc aaccgcgccg caggcagcgg gcaaaatcca tactgatttt
    28861 gaaaaaggct ttatccgtgc acaaaccatc tcgtttgaag atttcatcac ttacaaaggt
    28921 gaacaaggcg cgaaagaagc aggcaaaatg cgtgcagaag gtaaagatta catcgtgaaa
    28981 gatggcgatg tgatgaactt ccttttcaac gtctaattaa tttattgtct catgaggttt
    29041 cgctaaatct catgaagagc gcaaaagtat ataaaatcca cgcaattgcg tggatttttc
    29101 atttcaccga gtcctaatca ttcttgtaac atcctacact tttgtgctta ttgcctatag
    29161 tgaaccacga gaataattaa agacataagt gttcagatga cggatatctc attagcagtg
    29221 atataacgta agtttttgta tcactacaca tcagccccct tcacagctga tcaaaattga
    29281 tcacgctcca atatgctggc aagatgaaaa tgataaccag tagcacaata acaacgatag
    29341 gccagaaaac ccattgacca aaaatgaata tcgatgtttt ttgctttttc ttattagtaa
    29401 tgcaaaaatg gcgcatattg tttttgttat gcagatacat caagcgaaat tcccataact
    29461 aattaggatt attacaaaaa atcttcacc
//
LOCUS       DAJOSY010000051        29372 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJOSY010000051 DAJOSY010000000
VERSION     DAJOSY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.051"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(25..1422)
                     /gene="ccp"
                     /locus_tag="OHN27_002361"
     CDS             complement(25..1422)
                     /gene="ccp"
                     /locus_tag="OHN27_002361"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCQ2286791.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1827..3476
                     /locus_tag="OHN27_002362"
     CDS             1827..3476
                     /locus_tag="OHN27_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCQ2286792.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3527..4129)
                     /locus_tag="OHN27_002363"
     CDS             complement(3527..4129)
                     /locus_tag="OHN27_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCQ2286793.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4649..5548
                     /locus_tag="OHN27_002364"
     CDS             4649..5548
                     /locus_tag="OHN27_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2286794.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLTAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5597..6610
                     /gene="yhjD"
                     /locus_tag="OHN27_002365"
     CDS             5597..6610
                     /gene="yhjD"
                     /locus_tag="OHN27_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCQ2286795.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7021..8343
                     /locus_tag="OHN27_002366"
     CDS             7021..8343
                     /locus_tag="OHN27_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCQ2286796.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8525..10585)
                     /locus_tag="OHN27_002367"
     CDS             complement(8525..10585)
                     /locus_tag="OHN27_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCQ2286797.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLSWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10655..11422)
                     /gene="pdeH"
                     /locus_tag="OHN27_002368"
     CDS             complement(10655..11422)
                     /gene="pdeH"
                     /locus_tag="OHN27_002368"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCQ2286798.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11654..12583
                     /gene="kdgK"
                     /locus_tag="OHN27_002369"
     CDS             11654..12583
                     /gene="kdgK"
                     /locus_tag="OHN27_002369"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCQ2286799.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12679..14175)
                     /locus_tag="OHN27_002370"
     CDS             complement(12679..14175)
                     /locus_tag="OHN27_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCQ2286800.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAVWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14396..15682)
                     /gene="dctA"
                     /locus_tag="OHN27_002371"
     CDS             complement(14396..15682)
                     /gene="dctA"
                     /locus_tag="OHN27_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCQ2286801.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15865..17853)
                     /gene="hmsP"
                     /locus_tag="OHN27_002372"
     CDS             complement(15865..17853)
                     /gene="hmsP"
                     /locus_tag="OHN27_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCQ2286802.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAKELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPGDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17935..21408)
                     /gene="bcsC"
                     /locus_tag="OHN27_002373"
     CDS             complement(17935..21408)
                     /gene="bcsC"
                     /locus_tag="OHN27_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HCQ2286803.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDVADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYSLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21390..22496)
                     /gene="bcsZ"
                     /locus_tag="OHN27_002374"
     CDS             complement(21390..22496)
                     /gene="bcsZ"
                     /locus_tag="OHN27_002374"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCQ2286804.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22503..24842)
                     /gene="bcsB"
                     /locus_tag="OHN27_002375"
     CDS             complement(22503..24842)
                     /gene="bcsB"
                     /locus_tag="OHN27_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCQ2286805.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWIRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24853..27471)
                     /gene="bcsA"
                     /locus_tag="OHN27_002376"
     CDS             complement(24853..27471)
                     /gene="bcsA"
                     /locus_tag="OHN27_002376"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCQ2286806.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27468..28223)
                     /gene="bcsQ"
                     /locus_tag="OHN27_002377"
     CDS             complement(27468..28223)
                     /gene="bcsQ"
                     /locus_tag="OHN27_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCQ2286807.1"
                     /translation="MNGRTGIAGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNL
                     LRLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTR
                     LSDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQ
                     QALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGE
                     YRSDALAAEEILTLANWCLLNYSGLKTPVGSKS"
     gene            complement(28231..28419)
                     /locus_tag="OHN27_002378"
     CDS             complement(28231..28419)
                     /locus_tag="OHN27_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCQ2286808.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28692..29312
                     /locus_tag="OHN27_002379"
                     /pseudo
     CDS             28692..29312
                     /locus_tag="OHN27_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="required for cellulose biosynthesis; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
BASE COUNT         6936 a         8050 c         7379 g         7007 t
ORIGIN      
        1 ttcctgttcg ggcgatcaaa ttaattattg tttatcctgc atatacggcg tgtacacccc
       61 gttcagactg tgcaggaaag ctacgatatc atccacatcc tcctgcggca gctctttgcc
      121 tacctgatag cgcagcatca gtttcaccgc cccgtccagc gtcggcacgt caccgcgatg
      181 gaagtacggt gccgttaaag caacgttacg taaaccgggt actttctgac gcaatttatc
      241 acgctcttct ttagtcacat tcatacgacc aatatccgcc gccgtaattt ccccaaagtt
      301 aaagtctttt ttcagcccca acggttcaaa ggaacgtcca ccgagaataa taccaccatg
      361 acaagttgca catttattat ctttaaataa ttgatagcct tttttctgtt gcgccgtcag
      421 cgcattttca tctccacgca accatttatc aaatggggaa tccggcgtaa ttaatgtttt
      481 ctcaaattca gcaatggcat cagtaatatt ttcgccactg aaaccttgcg gatagacgcc
      541 gaggaactgc gctttaagct gcggatcttt ttccagctta gcaataattt cgtcccagga
      601 tttcgacgcc atttcaatcg ggttcaacgg cggtccacca gcctgatcct gcaatgttgc
      661 cgcacgacca tcccagaact gctcaacgtt aaatactgag ttaaataccg tcggcgcgtt
      721 aatcggccca actgcgccac caacaccaat cgatgttttt ctgccatcga cgccccccgc
      781 attcaacgca tggcaatgag cgcatgaaat ggtgctatca gccgataaac ggggatcgtg
      841 atacagcgca aagcccaacg ccactttttg cgcatcggta ggcagttttt gcgggatggg
      901 ctgcaccggt tcattgcgat gctccggagc agtatcattg ctggcgtaat attccgcgcg
      961 ctgttttgca atccaggcca gtatttccgc ccgctcttca tcactcacct tacccgccca
     1021 gtgtagcgcg gtataacgcg ttggtggcat agtttcatac tgcatcaccc attcaatctt
     1081 attcagatcg ctttgcgaaa cgggtttatc agccagcagt gccgcacgca cggcctcgag
     1141 gttaaaagat ttatatccaa gcttaatgtc gtaatccatc aactgtttcg cgccaggaat
     1201 ataataatag gcgggtaatt ctgccgaagg cgtgtggcaa tagtcgcatc ctttttcgcg
     1261 gagaaagcct aaaaccttat tattttcact gacagcagat gcctgaacat cggcctgttt
     1321 actgcgttta ttatcgtggt accacacata accagataac cctaaatagc aaatcgcgac
     1381 gccagccagg ccgatcgcgg taatacgtga gaccattttc attattcttc cctcacggtg
     1441 gtgagttatt gtgacccaga aaattttcca gaatgctgca aacgtctctg cgagacaaag
     1501 ccatcataaa aaacagtgag atacgctttt tgataggaat caatcaatta catgaatata
     1561 aactatcaat aagatagcct gtgcctatta cccagatcag gaatggcata tcatgattgc
     1621 ggcgagagca ggattgaatg ttctgaaaat gaaaaacaac acgaaatccg tatttttata
     1681 aattccatgg atattagtgc gttatatttt ttgacaggtt aataaacgtg atccaccgca
     1741 cgctttgtcg cccaccaggc ggagcgaatg actaccctta aagaaaagcc cgataattag
     1801 cgacgaattt cggaggttgg atccttatgc tcaatcagaa aattcaaaac cctaatccag
     1861 acgaactgat gatcgaagtc gatctctgct atgagctgga cccgtatgaa ttaaaactgg
     1921 atgagatgat cgaggcagaa ccggaacccg agatgattga agggctgccc gcctctgatg
     1981 cgctgacgcc tgccgatcgc tatctcgaac tgttcgagca tgttcagtcg gcgaaaattt
     2041 tccccgacag taaaaccttt cccgactgcg cacccaaaat ggacccgctg gatattttaa
     2101 tccgctaccg taaagtgcgc cgtcatcgtg attttgactt gcgcaagttt gttgaaaatc
     2161 acttctggct gccggaggtc tactccagcg agtatgtatc ggacccgcaa aattccctga
     2221 aagagcatat cgaccagctg tggccggtgc taacccgcga accacaggat cacattccgt
     2281 ggtcttctct actggcgctg ccgcagtcat atattgtccc gggcggccgt tttagcgaaa
     2341 cctactattg ggactcctat ttcaccatgc tggggctggc ggaaagtggt cgggaagatt
     2401 tgctgaaatg catggccgat aacttcgcct ggatgatcga aaactatggt cacatcccca
     2461 acggcaaccg cacctattat ttgagccgat cgcaaccacc ggtttttgcg ctgatggtgg
     2521 agttgtttga agaagatggt gtacgcggtg cgcgccgcta tctcgaccac cttaaaatgg
     2581 aatatgcctt ctggatggac ggtgcagaat cgttgatccc taatcaggcc tatcgccatg
     2641 ttgtgcggat gccggacgga tcgctgctca accgttattg ggacgatcgc gacacgccgc
     2701 gtgacgaatc ctggcttgag gacgttgaaa ccgcgaaaca ttctggtcgc ccgcccaacg
     2761 aggtgtaccg cgatttacgc gcgggagcgg cctcaggttg ggattactct tcccgttggc
     2821 tgcgtgatac tggtcgtctg gcgagcattc gtaccaccca gttcatcccc atcgatctga
     2881 atgccttcct gtttaaactg gagagcgcca tcgccaacat ctcggcgctg aaaggcgaga
     2941 aagagacaga agcgctgttc cgccagaagg ccagtgcccg tcgcgatgcg gtaaaccgtt
     3001 acctctggga tgatgaaaac ggcatctacc gcgattacga ctggcgacgc gaacaactgg
     3061 cgctgttttc cgctgccgcc attgtgccgc tctatgtcgg catggcgaac catgaacagg
     3121 ccgatcgtct ggcaaacgcc gtacgcagcc ggttactgac acctggcggg attctggcaa
     3181 gcgagtacga aaccggtgaa cagtgggata aacccaatgg ctgggcaccg ttacaatgga
     3241 tggcaattca gggatttaaa atgtatggcg atgaccttct gggtgatgaa atcgcgcgca
     3301 gctggctgaa aacggtgaat cagttctatc tggaacagca caaaatgatc gagaaatacc
     3361 atattgccga tggtgttccc cgcgaaggcg gcggtggcga gtatccgttg caggatgggt
     3421 ttggctggac taacggtgtg gtacgccgtt taattggttt gtacggcgaa ccataatatt
     3481 tttacagcca gccgctaact tcctgctggc tgtaaaatta tcctcttcag gaggagatat
     3541 ttaacatcat tgccgcctgg gtgcgatttt tcacttccag acggcgatac agggattcca
     3601 gatgcgcttt taccgttccg gtactgatat tcagcgctct gccgatctcc ttatttgatt
     3661 cgcccgccgc taacatggtt aaaatctccc gctggcgggc gcttaacgat ttgagatctt
     3721 taatgtcctt ttccggcgtc gtccgccagt ctccaggcag aaacatcatc cccatcgccg
     3781 cactatttac cgccaacgca aatgtctcga cggttgaatc acgaggcaca atggccagca
     3841 cattaaaatg gataacttcc tgtaaccacc gtttattgca atccgtcgcc gtgattaaca
     3901 ccttaacctc aggaaactgc accacggttt tttgcagcaa ccagtagcaa aactcaccat
     3961 cctgatcgcc atcgagcata actaaggctt cagggtaact ttccagcttt tgccataact
     4021 cgtctgcctg actggccccc tgaatactca ctcctggaat acgctgctgt aaactgattt
     4081 tcattccatg aataaatatt gactgcctgt caaacatgac tatttgcata actgaatctc
     4141 cacctgaata cgttaaaaag acttaagtag tggaagggta ttacccgcga gaaaaaataa
     4201 gaattcgcca tttggcggtg gccattctac agagatgacg cgtagaaaat agttaccgat
     4261 ataaatagtt acagctaaac gcctgaaatt acatgtcgag ggcactattt aaaacaattt
     4321 tgaggatttc cttatattgg tggttagtac gcatgcaatt aaaaatgaaa ctccgcgacc
     4381 acaagcaaaa ataacaaacg gcaatgagac aaaaataagc acaaatagcc aacacgtcct
     4441 ctattcactt taaagggaat cgctgaaaaa tacgctctgt ttagggggat tcacctttct
     4501 cagaaagcta ttccgccctt ttcctgctga gaaatcgcca cattcgtcat gacaacattg
     4561 tgaaacccgg cattagatgt tagaaaaaac aataaacaat gcgatatgcg cgttactgtt
     4621 tcgctgtgaa taacaatcgg tcaaagaaat ggataaaatt cacgcaatgc agttgttcat
     4681 caaagtcgcg gagctggaaa gtttttcccg cgcggcggat ttctttgctt tgccaaaagg
     4741 aagtgtttcg cgccagatac aggcactgga acatcaactt ggcacccagc ttctccagcg
     4801 caccacgcga cgggtcaaac tcacgccaga aggcatgacc tattatcaac gagcaaaaga
     4861 tgtgttgagt aatctcagcg aactggacgg tctgtttcaa caggatgcca ccagtatcag
     4921 cggtaaatta cgcatcgaca tcccgccagg aatcgcgaaa agcctgttac tgccgcgcct
     4981 gtcggaattt ctctatctgc atccgggaat tgagctggaa ctgagtagcc atgaccgtcc
     5041 ggtagatatt cttcatgatg gttttgattg cgtgatacgc actggcgcgt taccggaaga
     5101 tggcgttatc gcccgtcccc tcggcaaact gaccatggtc aactgtgcca gtccgcacta
     5161 tctgacgcgc tttggttatc cgcaaagccc cgatgatctg acttcacacg caatagtgcg
     5221 ttatacaccg cacctgggtg tacatccgtt aggttttgag gttgccagcg ttaatggcgt
     5281 ccagtggttt aagtctggcg gcatgttgac ggtaaacagt agcgaaaact atctcaccgc
     5341 cggtcttgcc ggtctgggga ttattcagat cccgcgcatt gccgtgcgcg aagccctgcg
     5401 tgccgggcgg cttattgaag tgttgccagg ctaccgtgcc gagccgctct ccctttcgct
     5461 ggtttacccg cagcgtcggg agctttcccg gcgtgtaaac ctgtttatgc agtggctggc
     5521 tggcgtaatg aaagagcacc tggactgacc gactatactt tttaagaatg accacgacag
     5581 aaggacaaaa gagcggatga cgcaggaaaa cgagatcaaa cgtcccaccc aggatctgga
     5641 gcacgagccg attaagcagt tggataatag cgagaaaggc ggcaaagtta gccaggcgct
     5701 ggaaaccgtc accaccactg ccgaaaaagt ccaacgccaa ccggttattg cgcacctgat
     5761 tcgtgcgaca gaacgcttta acgatcggct gggtaaccag tttggtgcgg ctatcaccta
     5821 tttctcattt ttgtcgatga tcccgatttt gatggtgtcg tttgccgcag ggggctttgt
     5881 gctggcctcc catccgatgt tgctacagga tatcttcgac aaaattctgc aaaacatcag
     5941 cgatccgacg ctagccgcca cgttgaaaaa caccatcaac accgccgttc agcagcgtac
     6001 gactgtaggg cttgtcggcc tggcggtggc actttattcc ggcatcaact ggatgggtaa
     6061 cctgcgtgaa gcgattcgtg cccagtcgcg cgatgtctgg gaacgctcgc cgcaagatca
     6121 ggagaagttc tgggtaaaat atctgcgtga ttttatttcg ctgattggtt tgttgattgc
     6181 gctgattgta acgctttcga tcacctcggt tgccggttcg gcgcagcaaa tgattattag
     6241 cgccctgcac ctgaacagca ttgagtggct gaaaccgacg tggcggttga ttggcctggc
     6301 gatttccatc ttcgccaact atctgctttt cttctggatc ttctggcggt taccgcgcca
     6361 ccgtccgcgc aaaaaagccc tgattcgcgg aacatttctg gctgctattg gttttgaagt
     6421 gattaaaatc gtgatgacct acaccctgcc atcgctgatg aaatccccct ctggcgcagc
     6481 atttggttcc gtgctggggc tgatggcatt tttctacttc ttcgcccgtt tgacgctgtt
     6541 ttgcgcggcg tggattgcca ccgccgaata taaagacgac ccgcgaatgc cagggaaaac
     6601 gcagccttaa aataggccgg atgcggcgta aacgccttat ccggcatacg attttattcg
     6661 gcctggctcc ccgtaggccg gataagatgc gccagcatcg catccggcta taatgcgcac
     6721 ataacctctt gaaactcttc cccagagcct cttcagccat ctatttggga gcaaacaatt
     6781 tcattccaac tcataacccc agcatataaa tccagttggt aacttttatt taacctgaaa
     6841 ccagttttat ccactattta taaaattatg tgaagcattt catagaagaa aaatcactgg
     6901 cctaaacatt atcccctttt tgcctggttt ttgaccattt ccgcgatttg ttacacattg
     6961 aaatatcact tttgctgtgc gtaatatggc tattcgttag ccaaaaaata agaaaagatt
     7021 atgcaagcaa cagccacaac actcgaccac gagcaagaat acacgccgat caactcgcgt
     7081 aataaagtcc ttgtcgcctc tctcattggc acagccattg agttcttcga cttttacatt
     7141 tacgccactg cggccgttat tgtgtttccg catatcttct tcccgcaggg cgatcctacg
     7201 gcagcaacgc tacagtcgct cgccaccttc gccatcgcct tcgtcgcgcg ccccattggc
     7261 tctgccgttt ttggtcattt tggcgatcgc gttgggcgta aagcgacgct ggtcgcctcg
     7321 ttgctaacga tggggatttc gaccgtggtg attggtctgc tgccaggcta tgccacgatt
     7381 ggtattttcg ccccgctgct gctggcgctg gctcgatttg gtcagggtct gggcttaggt
     7441 ggtgaatggg gcggcgcggc gctgctggcg actgaaaacg ccccaccgcg caaacgtgca
     7501 ttgtacggct ccttcccgca gctgggcgca ccgattggct tcttctttgc caacggcact
     7561 ttcttgctgc tttcctggct actgaccgac gagcagttta tgagctgggg ctggcgtgtg
     7621 ccatttatct tctcggcggt gctggtcatt atcggcctgt atgttcgcgt gtcgctgcat
     7681 gagtcgccgg tgtttgagaa agtcgctaaa gcgaaaaaac aggtgaagat cccgctgggt
     7741 acgctgctga ccaaacatgt tcgcgtaacc gtactgggta cgttcattat gctggcaacc
     7801 tatacgctgt tttacatcat gacggtctac tctatgacct ttagtaccgc cgccgcgcca
     7861 gttgggcttg gcctgccgcg taacgaagtg ttgtggatgt tgatgatggc agttattggt
     7921 tttggcgtga tggtgccagt cgctggatta ctggctgatg cctttggtcg ccgtaaaagc
     7981 atggtaatta tcaccacgct gatcatcctg ttcgcgctgt tcgcctttaa cccgctgctc
     8041 ggttctggca acccgattct ggtttttgcc ttcctgctgt tggggttaag tctgatgggg
     8101 ctgaccttcg ggccgatggg cgcgctgtta ccagagctgt tcccaacaga agtgcgttac
     8161 accggagcat cattctctta caacgtagcg tcgattctcg gggcttccgt tgcgccgtat
     8221 atcgcagcct ggttgcagac taactacggg ttaggtgcgg tggggttata tctggcggcg
     8281 atggctggct tgacgttaat cgccctgctg ctgacccatg agacgcgaca tcagtcgttg
     8341 taataagagt gaaaagcctg atgcgctacg cttatcaggc ctgaatttcc atacaatata
     8401 ttgaattcgc aaggatttgt aggccggata aggcgttcac gccgcatccg gcatgaacaa
     8461 agcacacttt gccaacaatc taaatctgtt taattgccgg atgtcagaca tccggcaatt
     8521 atcatcactt cttcatctgc gacaaaatcg tccgacactg attagcctcc ccttcggaag
     8581 gtgagatcag tgccagtaac gccgccgccg gtgttaccag cgttgccagt gccgcagcaa
     8641 cagcaccacg ggcaatcagc ggtccggctt tcaccccagc ctgcggattt ttaaacgtcc
     8701 cccgcacata cagcggcgaa cgcagtgtga taatccgaat tcctttactc tccggatcaa
     8761 tagtcaaatc cagctgttcc gaagcaaaac ttgccgtgcc ggtaacatta atcaacgcgt
     8821 tctcagtatc gaaagcaaaa atctgcgggc gcgccacgcc gttggcaata ttcagattcg
     8881 ccgccgcgca gttcacccgc acctcatcat caccaaatat cgcaccgaca atgtagttgc
     8941 cgacattcag cccaacaatc tccatcaggt tgcggctcac cagcccgtca ttcatcaaca
     9001 gtttcaggtt gccgttactg ttgcctaaaa gtgccgccac cgagttaccg ctaccgcgta
     9061 gttccgcgtc accgttcatt tcccccagcg tcttctgcat cagttccaca tcgggcatca
     9121 gttctttcag tttcagtcga cgagcctgaa tatctgcccg cccctgcatc ggctttttat
     9181 cgccttccag atgaatattc gccgcaatgc tgccgcccgc catgccaaat ttcagcggtt
     9241 gcaggcgcag gtcagcattt ttgaggatga tatgagtaga aagatcgcta atcggcaggc
     9301 tactgccatg ctcaatgcgc cgccctttga agcgaacatc ggcatccata acgtcccatt
     9361 tatcggtttc gaagcggtca taaggcagca ctttgcccgc aggctgaacg cttttttcgc
     9421 ccttcttctg ttcagaccgt ttcgactttt ctgccccttt cccggaatca acgccaatca
     9481 acggtcccag gtccgccagc cgcaattgcc gcgactcgac atcaccttcc agttttggtc
     9541 gtggctttcc ggtggtgtag accagagaac cgtggatatc gctatcacca attcgcccat
     9601 taaaaccgcg ataatcaaag accgacgatt tttcagtgtc gattttcgct accagccgac
     9661 catccgtttc aaacggcggg gtatcgggca gcagaacgcc cgtcagttca tagagatcac
     9721 ccagtgaatc gccagaaaat ttaagccgta aatcgacacc gcccatcttc attgggtcat
     9781 tcacgacgcc atcaaaagca acacgggtgt taccagagcg gaaatcagcc tgtaccggaa
     9841 acggcgtccc ttcgccacgc agcgccagca taccgcctat ttttcccgta cccgtgagcg
     9901 gttcaccgtt atatcgtccc tgcgccttca ggccaaaaac gtaatcgccc accttttctt
     9961 tatccgcttt acctttcgat ccagtaactt cgctgaacgg cagcggcttg cctaagggat
    10021 caacaaaaat ctccagatcc gctttgctta ctttgtcatc aatggcgatc cgcccttgat
    10081 cgaaaagaat attatccagc cgaaacgacc atgccgacgg ctttgcattc gcgtctttgt
    10141 tatcatcgtt ggcaagatta aacgtccagt tattgttctt ttcagagagg cgaatcaggc
    10201 gcgcgtcggg cttttcgagc ttgatccacg acagccagac cgttttggtc agcagcgcca
    10261 gcggggccag cgttgcctct acgcgtggca aatgcaccat cgtgacttcg ggaatatccg
    10321 gtggattgcc aagaatgatg tcttccgcgt gtacatgggg ccacggcacc cagctgcgcc
    10381 agccagtttc ttgtttttgc cgctcccaca ccacgcccag atcgccacgg atagcgaacg
    10441 gacgattcaa ctccgcagag actttctggt tgatagtcgg tttgagtcga ttccagtcaa
    10501 aagttgcaat caaaatgatc gccacgacaa tcaacaacaa gaaagcccct gaaatcgcag
    10561 cggttatttt gcctgccttg ctcatcgttc gcttcctcat attcttcctg tgccagtcct
    10621 aaagatagtc cagccaggcg gaaaatgagg cagcttatag cgccagaacc gccgtattca
    10681 gcgtttctat cggtgccggg cgtgaaagaa accagccttg tgcggcgaat gcgggcgagt
    10741 tctgaacatc acgccactct tccggcgttt ctacgccctc gacaatcacc ccgcgacaat
    10801 agcgattcat tagatgtaaa agctgagaaa agagtgtgcg tccttccggc gactgacgca
    10861 gcatcacaaa cagttctcgc gcgattttga tgtagtcata acgcacttca cttagcgcag
    10921 agaaatttgc catcccggta ccaaaatcat ccagccacag cgggccaaat tcacacatcg
    10981 aggcaaaggt tgaatctttc ggcagacgga tatgctccac cagttcgaaa cgcagccagg
    11041 gaagacgctc aatctggcgc aggatttttg gttgctgacg cagggcgatg agcgtagggc
    11101 catcaatatt gaccgatgcc agcaggccgt gctctataaa gaagtcggct ttttgcgcca
    11161 gcaaatcaat ctgctctttc acaacctcca tccgatggct gacggtgatt tcagtaaaat
    11221 agcgatccgg cggcaggcgt tgcgaagggt tcaagggatg cgtgaccacc gttaatagct
    11281 ccacggccat taaccgcccg catgtttgat agatcggctg ccaggtgtaa gcacgctcac
    11341 actgcaacca aaaacgccgt tcctgcaagc tctcgatgct tgcttcaggg ttgcttattc
    11401 gctggataac ctgccttatc atcaaagatg tcctgtgttt aaaagtgatg ctgcccggac
    11461 tcgacaaaga ttatcggcgc gtaagggcag aactttaccg cctggccggg gtaaatttcc
    11521 tcactccaca acggaatctt tcaggaatac gttctggctc aaaaaaataa tggaacactg
    11581 ttttaatatg gttgaccagc aaaccaccac agcgcaaact aacgctaatt ttttacagat
    11641 caggttcacg actatgtcca aaaagattgc cgtgattggc gaatgcatga ttgagctttc
    11701 cgagaaaggc gcggacgtta agcgcggttt cggcggcgat accctgaaca cttccgtcta
    11761 tatcgcccgt caggtcgatc ctgcggcatt aaccgttcat tacgtaacgg cgctgggaac
    11821 ggacagtttt agccagcaga tgctggacgc ctggcacggc gagaacgttg atacttccct
    11881 gacccaacgg atggaaaacc gtctgccggg cctttactac attgaaaccg acagcaccgg
    11941 cgagcgtacg ttctactact ggcggaacga agccgccgcc aaattctggc tggagagtga
    12001 gcagtctgcg gcgatttgcg aagagctggc gaatttcgat tatctctacc tgagcgggat
    12061 tagcctggcg atcttaagcc cgaccagccg cgaaaagctg ctttccctgc tgcgcgaatg
    12121 ccgcgccaac ggcggaaaag tgattttcga caataactat cgtccgcgcc tgtgggccag
    12181 caaagaagag acacagcagg tgtaccaaca aatgctggaa tgcacggata tcgccttcct
    12241 gacgctggac gacgaagacg cgctgtgggg tcaacagccg gtggaagacg tcattgcgcg
    12301 cacccataac gcgggcgtga aagaagtggt ggtgaaacgc ggggcggatt cttgcctggt
    12361 gtccattgct ggcgaagggt tagtggatgt tccggcggtg aaactgccga aagaaaaagt
    12421 gatcgatacc accgcagctg gcgactcttt cagtgccggt tatctggcgg tacgtctgac
    12481 aggcggcagc gcggaagacg cggcgaaacg tgggcacctg accgcaagta ccgttattca
    12541 gtatcgcggc gcgattatcc cgcgtgaggc gatgccagcg taaacgcagc atatgcaaaa
    12601 ggctggataa agtgttaccc ctctatccag ctcatccata aatacctgat ggttgtgcta
    12661 actcatcacc atcaggcatt actgtgcagg tggaatatcc gtcacttcag gatgtacgtc
    12721 atccgtggcg acagaggtgg tcgcagcggc ggtagatggg gccatgattt gatcccagac
    12781 cgcctgcaac gctttcatgt taaattccgg ctcgcctttc ggctgcagca gtattaacgc
    12841 catatcattc gacaactgtt gacgcagatc ctgatttaac atctctaccg tcaggctatt
    12901 aaggaaatcc tgccgcagtt tctgatattg ctccggcgcg atatcgacaa cctgattttg
    12961 caacgaacgc atccgctgac ccatcagaat atcggtatca gctcgtgcat aggcggcaaa
    13021 cagtttctgc agctccagtt tcttttgcgc cactaacgca ttgaactctt cttccggcag
    13081 acctttatcg cgaaccttcg ccagttcacg cgccaccaga ttaaggttgc tgttcagctt
    13141 gtcgtttggt gattcgatgt tgatggcaca ctgcgcacgc agatacagca cacggcagtc
    13201 aaatccaaga ccgatgtctt tgctgttact ggcacttaac gcttgctgaa catgccagaa
    13261 cagcgcctcg cgggccaggt ccgcacgcca gtagcgcagc agtgcggctg attcacgaat
    13321 cggctgccac ggcgtatccc acatgataga taaccggtcc tgacgcaccg cgtcagtcat
    13381 aatactcacc gcttccgcac gcagcggaga aagcgtcggc accggagctg gcgtttcacg
    13441 tttgcctttc agttcgccaa acgttttgtt tatttggtcg acaaccgagc gcgcatccac
    13501 gtttcccacc accagtagcg tcattgcatc cggggtgtac catttctgat agaaatcttt
    13561 aattttttcc gcttcaacgg gttgtttcag cggatcggca ggatcgtgac ctaacaaggt
    13621 tgaccctttc agacgatagc gccaccagcc ctctttagta tcggcaggcc aggttgccac
    13681 catgtcctga ctttgcagcg cgtggttgat ggtttctggt gtgatggtca atttgccagt
    13741 ggcatttgcc agataagaga gcgcttcttt cagcaaatcg ttacggttat tgggcaaact
    13801 cagattaaac agcgtggtgt cataagagac aattaccggc ggcatcgggc gtttagggtc
    13861 gatcccctgc tgccacaatg aacgcgcctg tgctgcgtca aggccaccgc tttgcgttag
    13921 cgcaatacga gggatggcgt gactgtaacc gctctgttgt gtactttcgg cgagcgaacc
    13981 ggtattaacc agcaggcgaa tttcaacacg atcgctggga cgctgggggg tggtcagcac
    14041 ttgccactgt aaaccgttgg aaagcgtccc ctgttgccat gctggatcag gctggagcgc
    14101 atctgcctgc acatagccag cagtggccat catcagcaaa ccgcccgcta aaagtcgaat
    14161 ttttgtgccc tgcatgtgaa cccctgatca actatcctgg taataaaaaa actgcccgtt
    14221 gtcggtcagt ctaaatgacg ttaaaaacac ttcgtgttag accgcaagaa catgaaaatg
    14281 tcacggaaga agtgaaataa acctgaactc acccaggtgg taagttcagg cacaggggtc
    14341 aattatgcgc aaacacccgc actcggggaa gggagtgcgg gcataagtga tgagattaag
    14401 aggataattc gtgcgttttg ccatccggcg cacgattatt cagcacatcg tccagttttt
    14461 tgtggtccag ttctttcacc cacttagcaa cgacaatggt cgctacgccg ttaccgacca
    14521 ggttagtcag cgcacgagct tctgacataa agcggtcgat accgaggatc agcgccagac
    14581 ccgctaccgg caaatggccc accgcagaga gcgtcgccgc cagcacgata aagccactac
    14641 ccgttacccc tgccgcccct ttagaagaaa gcagcaacac gattaacagc gtgatttggt
    14701 ggacgatatc catctgactg ttagtggcct gggcgataaa caccgccgcc attgtcaggt
    14761 atatcgatgt gccatcaagg ttaaacgagt agcctgtcgg gatgaccagc cccaccaccg
    14821 atttacggca gccgagtttc tccatcttgt cgagcatacg cggcagcgcc gactcggaag
    14881 atgaagtccc cagtacaatc agcagttctt cacggatgta gcggataaat ttgaagatac
    14941 tgaaaccagt cgctttagcg attgaaccca ataccagcac cacaaacagg atacaggtaa
    15001 tgtagaaaca gataatcagc tgccccagtt gcaccagtgt gccgacgccg tatttaccga
    15061 tggtaaacgc cattgccccg aacgcaccaa taggtgccag acgcatgatc atattgatga
    15121 tgccgaagat gacctgcgag aaactttcga tgacgttaaa aatcagttgg cctttgctgc
    15181 ccagacggtg gagcgcaaaa ccaaacagta cggcaaacag cagcacctgc agaatgttac
    15241 cgctggcaaa tgcgccaatg acgctcgccg ggatgacatc cataatgaag gcgacaatgc
    15301 cctggtcttt cgcctgatcg gcgtaaaccg ctaccgcttt cgcatcaagc gttgccggat
    15361 cgacgttcat tccggcacca ggctgcacga cgttaacgat gataagacca ataatcagcg
    15421 cgatggtact gacaatttca aagtaaagca gtgcgactgc gccggtacga ccgaccgcct
    15481 tcatgctttc catgcccgca atgcccgtta cgacggtaca aaagatgaca ggagcgatga
    15541 tcatcttaat gagcttaacg aagccgtcgc caagcggttt catttgctcg cctatttcag
    15601 gatagaaatg gccaaggaga ataccaatgg ctatcgctgt caggacctga aagtaaaggc
    15661 ttttaaacag agaggttttc atagggtgtc ctttagtaaa accacaggtc ttgtaaggtt
    15721 atggggtacc tgcggcctta aaataacacc cagacaacat cacagaaatg tacctggatc
    15781 ataattgaaa caaaaaggtt aaaaagtttg agctggctcg cacaaaccag cactttttaa
    15841 agttttgtaa tcagtttggg gtagctactt ttcttccagg taactctctt cgaagatttc
    15901 aatagggagt gggcgagcaa aaaggaagcc ctgggcaata ccaacgcccg cttttgccag
    15961 ccagtcgcgt tgtgcttcag tctccacgcc ttcggcaatc atttgtaagt tcaggctctg
    16021 cgccagcatg ataattgcag caatcatgct gctatctccc ggcaagcctt caacaaacat
    16081 tttgtcgatt ttcagtacgt cgattggcaa cgatttcata tgctgcagct gacgcagccc
    16141 tgcgtagccc atgccgaaat catccagcgc cacccgaact ccggcattgc gcagcggacg
    16201 gaggattgcc accgcagcat gagggtcgtc aatacgtcgg ctttctgtca cttccagaat
    16261 cagtgttccc ggctgaatgc gatagcgggt taacagttcc agcatatccg ccaccatatt
    16321 cgggtgcatc agttgcagcg cagagaggtt taccgacaag ggcagcataa tgccgcgctc
    16381 ttgccaggct gcaagcaatc gacaggactc ttccagcacc cagtgaccga cggtaaccat
    16441 cagcccacag cactcaatgc gatcgattaa gccatccggc aggtcccaac tgccatccgg
    16501 ttgctggata cgcagtaaca cttccgcact gaccagttta ccgctggtca tctcgacctg
    16561 tggctgtaac caaatagcaa actgatgatt ttccagtgca ttaaggatat cgctctcttc
    16621 cgtcaaccgc ttctgggcgg cttccatctg ctgcggatca aagaactgaa tctgattctt
    16681 gcctttatgg cgagcggtaa atgccgcaga aatagcgcga ctgtaaagct gttcggcggt
    16741 gagatcgccg tagaacatcg ccacgccaat gctacagtgc ggacggagtt gaatacgttc
    16801 aatcggcagg cgctcgctca tgatagtgag cacttgctga cctaaggtga ttgcgtgcca
    16861 cggttcctgt acaccgttgg caatgacagc aaagtcataa ccgctaatct gcgcgaggat
    16921 catacgtggc gacagtaccg atttgagttt ttccaccagc gtcagcagca gaatttctcg
    16981 ttgcgcctct ttcagcacgc ccgcagtatc acgcagggtt tcacaggtga tgatcatcag
    17041 cgcggtggtt tgtttacgcg cgacaacctg ctccagcatc tccatcagca aggctttgtt
    17101 cggcaaatcc gacaccggga agcgcatcgc attttcgttc tgttcttcat aatggcgctg
    17161 cagcaattgc tggttgaggt tgtaactgcg caccaacata ccgatttcat cgtcctgatg
    17221 cagacgcggt aatgccagtt ggtgaccaac aagctccttg gctgggatgg cgttaagttc
    17281 gcgagcaata ttgcgtaacg gatgcaaaat caggcggtta atgcaccagc tgatggcgac
    17341 ggtcaggata agcgacaaaa gtaagtaaat ggtcactaac gttgagaggg tgctcatcac
    17401 gaacttatac atacggaagg aatccgcctg tagtaccaga taggcaattg gctgcgggtt
    17461 tgccggacgt tcgagcgagt aaacgcccag cgagatttga accggtagct caaacaggcg
    17521 agtaaccatt accggcaccg ggcgctctgg aataaaactt ttacgcagcg cctggaactg
    17581 gttaggcagc actacatcgg cacggctgac cacgcctgcc ggtttaatgc tggcaagaat
    17641 agcttccgct tcgggaatat cgcctttcaa aatagctgaa gataagggtt cgcggacaga
    17701 gcgagcaatg ctttccagtt gcgtagccgt gttatagcga ttctgctgga ccagatggaa
    17761 cagcaaaacg gtgcaaaaaa taaaaacgaa caccaggaca acggctgcca ccattgccat
    17821 ctgcttgatt gttaacgagc gacttacgcg caaattgtct ccccccggaa atcccagatt
    17881 gccgcccgag tatacccgat cgcagcggca ttaagagagg cgctatctga aaacttacca
    17941 gtcggcgtaa ggtatcagcg gctgcggcgg taaatccatg tcaccctgcc atccggcggc
    18001 ggaataacgt acgtagagca ggaaatggct gggtgcgtaa tctttcgcct gctggatatc
    18061 aattgccgtg ccaacaaacc agttggaagt aacacgtcgt tcaagtaatg cccgcgccgt
    18121 gtagccgaag ccctgactgc tgccgccatc gttggattgc cgcgcagctt cttcctgcca
    18181 gtcggtcggg atcagattca tcagcgaata acgcggcatg gttttggtgc gtgaatgcga
    18241 ccacgagcca gacgcaccca gctcccacga ccagttttcc gtgcgctccc gccacatcac
    18301 cggtatggca aacgacaggt attcctgcgg actgtagtaa ccgccctgac cgagtgagta
    18361 gccactcaga tctttgtcgt aatgccagat catgttgttc aggccgattg tgacgcggcg
    18421 attgttctgg ttaatgacct tatagtaata gcccgtcatc cagcgcacgc gccagttatc
    18481 ttcgacattt ttaccggtta actggtcgcc actaagcgat gcccagacgc cgtttgcttc
    18541 acctttatcg tagctcagac ttagccccac accgtcggca cgtacgccac cccatttttt
    18601 cccggtattg ctcggggagt ctttttgccc accaaaggcc agcaaagaac tggagatggg
    18661 ccgacggtgg gcgttaacgg tgtaacccag cggcccgata tcatcgctgt aactgatgcc
    18721 gccgaccaca tccaccacgt tgaagcccat cggtgtggta ccgatatccc agctccagac
    18781 gtcatttcgc cagccgaccg ccacgctggc accggagtcc gactggctgc ggttgccgct
    18841 acagtcctgt aatgtacagg tgccccagtt gtcatcccat ttgccatcgg cattagtgga
    18901 gaaactgccg acgttcatat tgacgaaatc actgcgaaag aacatccgcc cgtcagaata
    18961 cggcgcatcc acctgcaaca tggtagtgtg cgctttcaga tcggagtaac caccggtgcc
    19021 gctcgaaccc cagtaatcgt gctcaagggt gacgttaaga tcctgctggc gatagaggtc
    19081 cgccacatcg ctgcgcacgc cacgtttcag ccagtcatct ttctcgtcgt tacgggtcag
    19141 tcgggtaaag gtgtcgttat cctgcggacg cgtcgtagtc acaccggatg cgaccatggc
    19201 gtctttgtag gtttccagcg cctgcgttgg atcgcccgcc tgcgcttcaa acttcgcacc
    19261 atcacgcagc accatcgcgc tttccatcga cggtggctga gattttgcct gcgggatcaa
    19321 cttattaaac gtccgctgcg ctgctgcggt atcgccaagc tgcgcctgtg ccagcgccac
    19381 gcgccgctgt gtgttcagcg aggcgttatc ggtagcgggc agtttcgcca gctggctacg
    19441 tgccgccgct ttgtcaccgg cagcaatatc cacttccgtc agaccaagaa tggcgtcggc
    19501 gttagctggc tcccgcgtca ggacattctg atatgcagcg cgggcggcgg tgtaatcacg
    19561 tcgttgttgc gcccagtcag ccagcgtgag gtcaatacgc gtggaaggtg gttgctggcg
    19621 cagcatcgct tccgcttctg cctctttgcc gctttctcgc aggcggttag cggtttccag
    19681 cacctgatcg ctttgcagtc gattaaccag ctcctgaata ttgctgttcc actgcgcacg
    19741 cggcaggctg ttgatatgcg ccagcgccgc tctgtcctgg tcatgaccag agagatacag
    19801 cccgtaagcg taaacctgct ccgggtcatt cggcttctgc tgcgccagat tgcgcattaa
    19861 cgtatcggcc tggctgcgtt gtccggcctg ccagagatcc tgcgaaagtc ggtaagtaat
    19921 ccatacgctg ccggggtcca gcgccagtcg ttgccgctga agtgctgccg cctgcgccca
    19981 tttgccctgg ttttccagtg cctctgcctg ctgtgccaga cggtcgtttt gcaggctgcg
    20041 ttcgatatca tcaatgctac gccgctgact ggcagagagc gaggcgataa acgcttcagc
    20101 tttttctggc gattgctggc ggtaaatatt tgccagcccg cgcacggcgt tagtgttgcc
    20161 gctgtccata cgcaaggtct gctgataata acgttcggcg gcgggataat cttttcgcgc
    20221 catcgccaca tcgcccagcc ccagcactgc ataactgtcg gtgttatcga cattacgcgc
    20281 ctgctggaac aggcgttctg cccggtcagg attattggct ttcagcgcag catcgccctg
    20341 ctggatcgcc agccagtagc ggtttacttt cagcagactg ttccatttgt cgttgttgct
    20401 gctgtgcggg tccagtgcga gggctttttc cagattcgcc actgcattgg cgcgatcgcc
    20461 tttctgagaa tacgcctggc ccagcgcccc cagagcttca ctgtctttcg ggttcgcccg
    20521 caccgcctgt tgtagttcgg gaatggcttt acccgccata ccagagtcca ccgccgctaa
    20581 accttgcgca cgagcgcgga aagcaggatc ggccagctgt ttttgctgtt ctgccagttg
    20641 cgattgcgca gccgccacgc tatcgccatc actaaagatc gagagatatt ttttcagcgc
    20701 cgacacactg gcatcactga cgggcatgtc tttaatctgc ccgtaccaga ttttagaggc
    20761 cccttcgcgc ccggcgttcg attttgccat ctgttccagg acggcaaaac cttcgtcacg
    20821 gcgatcgcta ctaaacagca atagcgccag attgttttgc aggcccgtat tgcccggtgc
    20881 atccgcattg atgcgtttta actgattaat cgcttcgcca cggcgagccg gaattttcgc
    20941 caccgtactc cagtactcga cagcaatgtc accttccggc ggcgcaccgt tgaacagttt
    21001 gttgtaactc gccacagctt cttctgcatg accggtcgtc gcctgcaatc gtgcctgttg
    21061 cagtgcctga cgaccatccg gcgtggaaag tagcatcgta gtccgcgacg atttatacgc
    21121 atttgaactc ggcgctaact gcgacagccg atcgagctgt ttttgcgcgc catcaatatc
    21181 gccctgacgt aacaaagaac ggaaacgggc ggcaacgacg tccgggttat tcggatcaat
    21241 aagttccagc cgatataacg actgttgcac cagatcttca cgatgggtcg cttcgcctaa
    21301 ccgaacttgc tccagcaact gttgctgagc ggttggtgct gcctcgacca tcggcatgac
    21361 ggccagaccg agggaaagcg tgaatatgtt tagtgtgaat ttgcgcattc ctggccccag
    21421 tcaggtaata actcaccttt tgtcgagaag cggaaacggt gttgatccca gccttgtcca
    21481 aacagggtca gcacatagtt gtaataggca tcgctgccgg gaaagttatc ggccacgcgc
    21541 tggcgctgaa cggcctgcgc atcgcggttt tgtaaaaagg gcagcatggc ggcagaaaaa
    21601 ccgactggtc ctttaccctg cgctttcccc gtagccacat ccactttttc cggcggataa
    21661 ccgtttttct cagtgaatgt cgccatcggt ttaaaccggt tgagcatccg cgctttttgc
    21721 ggatcgctgt caggcatcat gcctacccac atgtaaacgc ggatagcgtc gtagctgctg
    21781 atcaatgttt tttcggcttt tagctgccag cctttgtctt tctcatagcg cacccagtct
    21841 ggcgaaaaac ctttcggggc ggtttccagc aataaacgtt gattggtttc gcgcagcgtg
    21901 gtccacggcg cgccaaagcg ggtgaaatac tgcgccagcg tcggcggcag gtagctgggg
    21961 ttaaaacgcc agctgttatc ctcagcaaaa cccactttgc ccggtaacaa catggaaccc
    22021 agcccaggca ccgtcaccac ttcctcccgc gcgatacgtt ttagcaacgc gctgccgatg
    22081 tcggtataac gctgctcttt ccacaaacgc cccgcctcca gcaacgacca ggccatccag
    22141 acatcaccat cggaggccga attgctgtcc agcacttccc acttactgtt ctctttcttg
    22201 ccccacagcc aggcgggcaa acgttctttt aaagaaccct gagcgagatt gttctgcgtc
    22261 cagtcgagaa tattatcgaa agctgcacgg tcgttagccg ccagggcaaa gaacatgccg
    22321 taactttgcc cttcggaggt ggtgattttg cgcgcgtcgc tggggtcgat gacgcgccct
    22381 tcctgactga tgtaatcctt tttaaactgc tcccaggcag gccaggtaca ggctgcctga
    22441 acactaaagg cagccagcag cagcatcgtc acgattccac tacgcaacac attcatcttc
    22501 aattactcgt tatccgggtt aagacgacga cgactaataa ttcgcagcag acgccacagt
    22561 acccatgcca gcaatatcac actgatagcc gccagcaccg ccagcagaat cggatggttt
    22621 gccagcgcat accacaagcg ctcgaaccac ggcagatgac ctacgtaata aacgtcgcca
    22681 acacgtaggc tgttgatacc ggactcgcgg atcaccgcga ccgaaccgaa catggtggcg
    22741 cgtttgccgc tatcgttcac cgcatcgtta agcatttcat aaccgcgtgg gctatctgcc
    22801 aacagcgcaa tcacgctgcg ctggtcgtta tacggcgact ggaagccaat caccgccgcc
    22861 atcgcaccgg aagaggtcag cgttgaccgg gtttctgccg cgcgatcgct ctcgtccggc
    22921 acaatgccgg ggaacggggt ctggcgcatc ggtgttttca cccagctttc tgtcgcctgc
    22981 accaataggt cgatctgctt atcgtctttc agtttgtccg ggataccacc gatgatcatg
    23041 atatcggcat ctttgccctg aatggtgctg ccatcatcgg tcaccgtcag attaatcgcc
    23101 gggaagcccg tctgtgcgcc gataaaacca acagtattca gcaacgtttc catctgtgct
    23161 tcgttaggcg ctttcggcat cacggtgatg gtttgcgaca gatccgccat ccggctgaat
    23221 gggaagcccg cgttagcaaa ggcgcgtaga tccggcatcg ggatgaagtg gtaatacttc
    23281 gagaagtcga tggtggagtc gtcaccaatc accacatgat tctgcaccgg ctggaaggta
    23341 atacagttat ccaccgaacc gcccggcatc gggttcatat actcaaagtc gaagcgcagc
    23401 tggttggtcg cgcccagttt cagcgccgga atagagacat ctgttttgcc atccagcaaa
    23461 ccttgtaata ccggaatccg cagcagcagg cggttcgcct cctgtttgct gctcaggttg
    23521 aaggattgca ggaactggtt attcaggctg atatccatcc gcgaactgtc tttcaccggc
    23581 ggcatggtgt agcggtaatt aatatccata tcaatgccgg tactgcgcat caggtagaga
    23641 tccggcggca ggtttagcga aacgttaatc gctgctggct caagaccgct ggattgtaac
    23701 tgttcttcat aggttttcag ttcgccaaat gtgaccggac gatcggtacg tatccagttc
    23761 ggcgcatcgt acggcttacg cggtagcagc ggtttcactt cattcactac cacgctttca
    23821 ccacggaaca gaatgttacc ctgagcgata cctttcgctg cctgcaacag gtctttgtcg
    23881 tcacgaccaa acaccaccag cagtttgacg taaggattct gcggatggtt aatcatctca
    23941 atcaccgggg cttttaccgc cggatgatcg cgcaggaagt ccggccgttt gtcgttggtt
    24001 gcaaagacaa tggcattacg atccggcagt tggttataga gcaccgggaa gttctgccca
    24061 cgccagccag aacgcgaacc aaaccacgag gcgacaatgg cagaggcttg ttgcagccca
    24121 acatccggcg cacccgcaaa gaccatcggc aaggtgttag tacggttatc gcgcgggtca
    24181 aagaatggca ccgggaagtg tgacaggtca ttcttcacat tcagggtctg ataggtcaga
    24241 tccagtccac tgctccgccc aacatccagc caaagcgtgg tgctggccgg gttttcgcac
    24301 acgtcctgat aatggccgac aaactccagc cgtacacggt tgaagtcggt aataaacagt
    24361 gggttaatgg gcatttgcgc cagcgttttt ttacccaact gttctttggt cactggcagc
    24421 acgcccatca gttcatcatt gagataaacc tttaactgcg actggacagg cagtaacgat
    24481 ggcgatgggg tgtattcgag gttgagcatc gccttcgtca ccacttcatc gctgcgcata
    24541 ccaaactcaa tgctgccgtt cgggttaatg ccacgtagca ccatgctgcc cggcggcggt
    24601 gcaatttgcg caaaggtcag cttcacatca cgcgaaggac cgttctgcgc cacgactggc
    24661 gcatcagcgc cctgcacgcc cggcatcact tgccccacct gcggattttg ttccgtctgg
    24721 gcggctacag ctggctcagc attgatcagt ggttgcgttg ctggcgtcgc ctgcgtcatg
    24781 aaagagggga acgcactcat ccccatagcc actgcacaaa tccagaatag ttttcttttc
    24841 atcgcgttat catcattgtt gagccaaagc ctgatccgat ggttgtgccg tttcgctccg
    24901 ctccgggcgg cgcggaataa acgatacaac ccaggaaacc agagaagtca gcacacggaa
    24961 tatgcccttc accgaagaag gcgcaaactc cgccagatgg cggtagccac ggaagccgag
    25021 cttcagaata tccagcagac tttccagcgg cttatcttcc gggtagctgt cctgccagag
    25081 cgcccatgta tccgcacggg caaacgtaca ctgcacaaaa tcgatatgtt gctgggtggt
    25141 gagcggcatt aatttcagcc caacttcatt acccatcacg cgcgccacct gggtcgggaa
    25201 gacgtattcc tgctgaccgc gtttaagcaa cagattcact ttctgccctt ccagaatctg
    25261 cgcctgaccg ttgatcttga tccccaaacc accgtcggag aaatcctgaa cggtacacga
    25321 gaagaggtga ccatcttcgc gggcaattgc cgcgggcatc gtcatctcca cgcggtgcga
    25381 tcggcgtacc tgtttgcttt ctaccgatac tgcaactgcg ccgccaagaa caatcaggtt
    25441 gtagaatacc cacaccatac tgacgaccac ggtgagcatc tcggttggcg ggccatagaa
    25501 gtagcgccag atgcctaccg caacgcccac caggttgagc aggacaagga agatgtaggg
    25561 ccgcgagatc acccaatcga cgtactcttc ttccaccagt ccacctttgg cggtgacgtt
    25621 aaatttgcct ttgtgcgggt taatcagcgc caccagcgtc ggtggtgcga tataccacgc
    25681 cagcaccgtt tcgtagattt cactccagaa agagtggcga tatttgccct ggatcttgga
    25741 gttggtcagg ctggcatgga tcatatgcgg cagcacgaat agggcgatca tcaacgctgg
    25801 cgcatagatg atgtaggcat gaagcagcag gaacgccagc ggcgcagtca ggaagatcag
    25861 ccgtggaatg cccgacaaga agtggaacat ggcgttgacg tagcacagcc gctgagcaaa
    25921 cttcagtcct ttaccggtga gcgggttatc gagacggaag atttgtacca tcccgcgcgc
    25981 ccagcgaata cgctgaccga tatgcgccga cagactttcg gtcgccagcc ccgccgcctg
    26041 cggaatacgc atatacgcgg aggtatagcc acgacggtgc aaacgcagag aagtatgcgc
    26101 atcttcagtc acggtttcga cagcaatgcc gccaatttca tccagcggct tacgacgaat
    26161 caccgcacag gaaccgcaga agaaagtggc gtcccacata tcgttgccat cctgcaccag
    26221 accatagaac agcgtgcctt cgttcggtgt tttacgaaaa cgccccaggt tgcgttcaaa
    26281 cgggtccggc gagaagaagt ggtgtggagt ctgcatcatc gccagctgtt tctctttcag
    26341 gaaccagccc atggtcattt gcaggaacga tcgcgttggt acgtggtcgc agtcgaaaat
    26401 cgacacgaac tcgcctttgg catatttcag cgcattgttg atgttgcccg ctttcgcatg
    26461 ttcatgagtg gtacgggcga tatatttcac ccccacgttt tgcgcaaact ggcgaaactc
    26521 ttccctaccg ccgtcatcga ggatccagat gttcagcttg tctttcggcc agtcgatacc
    26581 cagcgaggcg taaatagtat ttttcaccac gttgagatct tcgttgtaag tcgggacaaa
    26641 gatatccacc gacggccaca gcgacatatc tttcggcaat ggcaccggct gacgattcag
    26701 cggccatact acctggaagt agccgagcac cagcacaatc cacgcgtacg tttcagcgaa
    26761 gagcagaata agcccgcaca ccaggctgac cggatcgtcc cagttcagcg tagaggtgta
    26821 acgccaccag atataacggc aagaaacggt cagcgacagc acaatcaaca ttagcgccga
    26881 gaagcgcccc ggcatccgcc gtacgatcag cgctacaccc cacagcagca tcaggaagat
    26941 aaactgcgcc agcgggttaa acggttgagt aacgcagatt aacgccagaa tcagcgagaa
    27001 ggtgacgatg ataccgagga tcaaccgccg cgccccggca ctcaaatgac cgagctcttt
    27061 tttctcatca agatgctgtg ttttatggct aacgcgctca ggcagctcgt tcatccattg
    27121 atggtaacgt ccacgaatat tttgcagacc tgaaaatgcc cgcctgcgcg gtttcggcgt
    27181 ttctttgcgc gatgcaccga tcaataacca gcatgtttga atgagataac ggaccgggtc
    27241 cagcggacgc ggacgcgagg cgttgatatg cggatacagg tttttatgtt ctgcgcgaat
    27301 acgctgccag cgcgggtgct ccagcggaat aaaaatccag gccaggatca tccagaaaca
    27361 gccgagcgtc gcgctgaaag ccgacgcacc gtggcgacga taatcgcgat aacgcccgat
    27421 cagccgcgcg ttgaccggcg ggattagcaa ccaccgggtc aggatactca tgatttactc
    27481 ccgactggcg ttttcagccc ggaatagttc aacaggcacc agttcgccag cgtcagtatc
    27541 tcttcagccg ccagcgcatc actgcgatat tcacctactg gttgcttagc cgccaggcat
    27601 tcagccatcg cttcatcacg atgaatgagc atcggcagta atcggcgctg gctttgcaac
    27661 caaagctggt aaatatcgtc ctgaacctga ctgccaatac ggaagttatt aatcaaaata
    27721 tgtgcgccat ccggcagcgc ttgctgatgc agtcggatat ggcagttggc atccacattg
    27781 acgattgcca gcgagtgatc gcacaaactc agcagctggt gggttatctg cgaggcatca
    27841 cgcggtaagt cgattaaaat ccactggtaa cgcccgctgg cttttagttg ctgtaagccg
    27901 gagcaaatat cgctcagccg ggtttgccag tgctgtggat tttcttgttc ttcaatggat
    27961 aactgaccaa aaggcagcaa atcgagctgc gaggtgtagc gcaacccggc gtcacgccag
    28021 tcctggccat ccagcatagc tctggcccag ccctgacggt gggtaaaatc aacgttaaat
    28081 gacaggcgca acaagttgtc cgggcaggca tcgaccacca ggacattttc tcccaacatt
    28141 tgtaatgacc aggctaatgc ggcggtgatg gttgttgtcc ccacgcctcc ccgcacccct
    28201 gcaatcccag tacggccatt caccaatccc ctacttttgt tgcgcaaact ctgccagcaa
    28261 cggccagcgt tttaatgccg cggccaactg ttcgcgttgg gaaatatcgg cataatcaat
    28321 atcaggcagt gaaaatgcct gctttaacgc cacaatatca ttctggaaga tatagcctat
    28381 cgcgggatca ggcagagtat ctggttcatt gttattcata ttgcttgatc cctatgaaat
    28441 aaccgtaccg ttcatgagac aattctaacc gtgagcattt cgccggttta ttgtttacgg
    28501 cgagacggga atttgctcac tttttgactt tccgttacgc aacagaaaaa gttaaaaacg
    28561 aaaagactaa aatcacacgt cttgtgacct tctaaattga ttcacatgct aaatctgata
    28621 agttttaatt tcaatggtag gtttatttct tagctttcgc tagtaaactg ataatcagac
    28681 aaaatggtga catgagggac attgtggacc ctgtattctc tatcggtatc tcatcattat
    28741 gggatgagct gcgacatatg ccagcaggcg gcgtctggtg gtttaacgtc gatcgccatg
    28801 aagatgctat cagtctggcg aatcaaacaa ttgcatccca ggctgaaacc gcacacgtcg
    28861 cggtcattag catggacagc gatccggcga aaatctttca attagatgat tctcaagggc
    28921 cggaaaaaat aaaattattt tcaatgctaa atcatgaaaa aggtctatac tatttgaccc
    28981 gtgatttgca gtgttctatt gatccccata attacctttt tattcttgtt tgcgcaaata
    29041 acgcatggca aaacattcct gccgagcggc ttcgctcatg gttggataaa atgaataaat
    29101 ggagcaggtt aaaccattgt tcgcttttgg taattaatcc cggaaataat aacgataaac
    29161 aattttcatt gttgcttgag gaataccgtt cactttttgg tcttgccagt ttgcgttttc
    29221 agggtgacca acatttgctg gatattgcct tctggtgcaa cgaaaaaggg gtcagcgccc
    29281 gtcagcagct tagcgttcag caacaaaatg ggtgatgctg ccaacttact gatttagtgt
    29341 atgatggtgt ttttgaggtg ctccagtggc tt
//
LOCUS       DAJOSY010000052        29333 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJOSY010000052 DAJOSY010000000
VERSION     DAJOSY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.052"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(187..1851)
                     /gene="glnS"
                     /locus_tag="OHN27_002380"
     CDS             complement(187..1851)
                     /gene="glnS"
                     /locus_tag="OHN27_002380"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCQ2286809.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2054..4000)
                     /gene="nagE"
                     /locus_tag="OHN27_002381"
     CDS             complement(2054..4000)
                     /gene="nagE"
                     /locus_tag="OHN27_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCQ2286810.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4333..5133
                     /gene="nagB"
                     /locus_tag="OHN27_002382"
     CDS             4333..5133
                     /gene="nagB"
                     /locus_tag="OHN27_002382"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCQ2286811.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5193..6341
                     /gene="nagA"
                     /locus_tag="OHN27_002383"
     CDS             5193..6341
                     /gene="nagA"
                     /locus_tag="OHN27_002383"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCQ2286812.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     KNADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6350..7570
                     /gene="nagC"
                     /locus_tag="OHN27_002384"
                     /gene_synonym="nagR"
     CDS             6350..7570
                     /gene="nagC"
                     /locus_tag="OHN27_002384"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCQ2286813.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7618..8370
                     /gene="nagD"
                     /locus_tag="OHN27_002385"
     CDS             7618..8370
                     /gene="nagD"
                     /locus_tag="OHN27_002385"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCQ2286814.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8767..10431
                     /gene="asnB"
                     /locus_tag="OHN27_002386"
     CDS             8767..10431
                     /gene="asnB"
                     /locus_tag="OHN27_002386"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCQ2286815.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIISLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10811..10887
                     /locus_tag="OHN27_002387"
     tRNA            10811..10887
                     /locus_tag="OHN27_002387"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10845..10847,aa:Met,seq:cat)
     gene            10896..10980
                     /locus_tag="OHN27_002388"
     tRNA            10896..10980
                     /locus_tag="OHN27_002388"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10930..10932,aa:Leu,seq:tag)
     gene            11004..11078
                     /locus_tag="OHN27_002389"
     tRNA            11004..11078
                     /locus_tag="OHN27_002389"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11036..11038,aa:Gln,seq:ttg)
     gene            11113..11187
                     /locus_tag="OHN27_002390"
     tRNA            11113..11187
                     /locus_tag="OHN27_002390"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11145..11147,aa:Gln,seq:ttg)
     gene            11203..11279
                     /locus_tag="OHN27_002391"
     tRNA            11203..11279
                     /locus_tag="OHN27_002391"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11237..11239,aa:Met,seq:cat)
     gene            11327..11401
                     /locus_tag="OHN27_002392"
     tRNA            11327..11401
                     /locus_tag="OHN27_002392"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11359..11361,aa:Gln,seq:ctg)
     gene            11439..11513
                     /locus_tag="OHN27_002393"
     tRNA            11439..11513
                     /locus_tag="OHN27_002393"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11471..11473,aa:Gln,seq:ctg)
     gene            complement(11667..12842)
                     /gene="ubiF"
                     /locus_tag="OHN27_002394"
     CDS             complement(11667..12842)
                     /gene="ubiF"
                     /locus_tag="OHN27_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCQ2286816.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            12988..14412
                     /gene="miaB"
                     /locus_tag="OHN27_002395"
     CDS             12988..14412
                     /gene="miaB"
                     /locus_tag="OHN27_002395"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCQ2286817.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14565..15605
                     /locus_tag="OHN27_002396"
     CDS             14565..15605
                     /locus_tag="OHN27_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCQ2286818.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15602..16069
                     /gene="ybeY"
                     /locus_tag="OHN27_002397"
     CDS             15602..16069
                     /gene="ybeY"
                     /locus_tag="OHN27_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCQ2286819.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16159..17037
                     /gene="corC"
                     /locus_tag="OHN27_002398"
     CDS             16159..17037
                     /gene="corC"
                     /locus_tag="OHN27_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCQ2286820.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            17062..18600
                     /gene="lnt"
                     /locus_tag="OHN27_002399"
     CDS             17062..18600
                     /gene="lnt"
                     /locus_tag="OHN27_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCQ2286821.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18678..20555)
                     /locus_tag="OHN27_002400"
     CDS             complement(18678..20555)
                     /locus_tag="OHN27_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCQ2286822.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLSLSGEPWRLLSSVFLHSSFSHLLMNMFALLVVGAVTERILGKWRLLI
                     IWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGTGTHHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREEFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLTLSPDETLLYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKEDFPAGDARGTISAMRFRPENGDVIFAD
                     SQGISRIRVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IVGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            21010..21918
                     /gene="gltI"
                     /locus_tag="OHN27_002401"
     CDS             21010..21918
                     /gene="gltI"
                     /locus_tag="OHN27_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCQ2286823.1"
                     /translation="MQLRKPAIAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            22088..22828
                     /gene="gltJ"
                     /locus_tag="OHN27_002402"
     CDS             22088..22828
                     /gene="gltJ"
                     /locus_tag="OHN27_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCQ2286824.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            22828..23502
                     /gene="gltK"
                     /locus_tag="OHN27_002403"
     CDS             22828..23502
                     /gene="gltK"
                     /locus_tag="OHN27_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCQ2286825.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23502..24227
                     /gene="gltL"
                     /locus_tag="OHN27_002404"
     CDS             23502..24227
                     /gene="gltL"
                     /locus_tag="OHN27_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCQ2286826.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24345..25280
                     /gene="rihA"
                     /locus_tag="OHN27_002405"
     CDS             24345..25280
                     /gene="rihA"
                     /locus_tag="OHN27_002405"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCQ2286827.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TVVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTSVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25364..27033
                     /gene="hscC"
                     /locus_tag="OHN27_002406"
                     /pseudo
     CDS             25364..27033
                     /gene="hscC"
                     /locus_tag="OHN27_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(27093..28544)
                     /gene="djlC"
                     /locus_tag="OHN27_002407"
     CDS             complement(27093..28544)
                     /gene="djlC"
                     /locus_tag="OHN27_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCQ2286828.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPIALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWLIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQTEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPFLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIVTRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28541..29248)
                     /locus_tag="OHN27_002408"
     CDS             complement(28541..29248)
                     /locus_tag="OHN27_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCQ2286829.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFELSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQSECPVTLKDVSDL"
BASE COUNT         7241 a         7619 c         7383 g         7090 t
ORIGIN      
        1 ctgtttcttg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca cgcaaactca
       61 atatattgca gagatcatgt aggcctgata agcgtagcgc atcaggcaat ttagcgtttg
      121 aaactgtttg tctgttcctt gccggatgcg acgtaaacgc cccatccggc atagcgaaac
      181 ttaaaattac tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac
      241 cggtttttcc gccgtagaat ggcggctatc gaggcagaag taaccttcac gctcaaactg
      301 gaatgcttta cccgcaaccg catctttcag cgacggttca gcaaagccct gtttgatcac
      361 cagcgattcc gggttaatca ccgacaggaa atcatccgca gcacctgggt taggcacgct
      421 gaacagacgg tcatacaaac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg
      481 aataacacct ttgactttac gaccatctgc cggatctttg cttaaggtat cggcgtcata
      541 agtacagaag atggtggtga tattaccttc ggcatctttc tcgacgcgtt ctgccttaat
      601 cacataagca ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt
      661 agcttcttcg cggaaatcgg cgcgatcaat ccaaatctca ccgctaaacg gcacctgacg
      721 gctgcccatt tccggtttgt tcggatggtt cggcatggta accatttcgc cttcgccctg
      781 atagttttcg ataaccagtt tcaccggatc gataaccgcc attgcgcgcg gcgcattttc
      841 gttgagatct tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt
      901 ggtcacgccg atgcgtttgc agaactcacg aatagaagcc gcagtgtaac cacgacgacg
      961 cagaccggaa atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac
     1021 cagcaggttc aacttacgct tggacatcac ggtgtattcc agattcaggc gcgagaactc
     1081 atactggcgc gggtgaacag gaatcgtgat gttgtccagt acccagtcgt acagacgacg
     1141 gttgtcctgg aactcaagcg tacacagaga gtgcgtaata ccttccaggg catcgctgat
     1201 gcagtgggtg aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc
     1261 agcaaattta atacggtaca gcaccggatc gcgcatcacg ataaacggtg aagccatgtc
     1321 gattttcgca cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa
     1381 cagcgccagg ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggttgcgt
     1441 cagggtgccg cggtattcgc ggatctgttc cggcgtcagt tcatcaacgt acgccaggcc
     1501 tttattgatc agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg
     1561 gacgttacca gaccagtgaa aacctaacca ctctacgtcg tttttgatcg actcaacata
     1621 ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata
     1681 gtcctgggcg atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc
     1741 attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc
     1801 atcgatgatc tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc
     1861 ctcaaagcgt aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact
     1921 gttagttttt taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa
     1981 gcggcagaga agatctccac cgcttgtcat tgttggatgc gacgctcaag cgtcgcatca
     2041 ggcataaagc agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc
     2101 cctgagcttt aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa
     2161 tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg
     2221 cgcttacctg cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg
     2281 tgtcgatacc catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt
     2341 ggttggtgtt gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg
     2401 tcggtttcac cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct
     2461 gatccagtgc cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt
     2521 ttggtacagc ctgcggtttt gctacaggcg cggcagttgc cggagttgct tcagctgacg
     2581 cagctgctac cggaccgcgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt
     2641 tcgcgccaac aatcacctga atagtctgtt tgttcagttt tactacacca gaagcaccca
     2701 gacgtttaca catcgtatcg ttaacgcggg cagagtcagc cactgtaagg cgcagacggg
     2761 tgatacaggc gtcaattgct ttcaggttgt cagtgccgcc aaccgcagca atatagttgg
     2821 tcgccagttg attgaggcct tcttcagtgt tgctgttggc ttcttcagta acgatctcgt
     2881 cttctttatc ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca
     2941 ccacgaagta gatagcgaag aagacaacac ccatcaccag cagcatccag acgttctggc
     3001 tggcggccgg caggttatac atcaacgcgt agtcgatagc ccccgcagag aaagagaagc
     3061 cagcatggat acccagcagt gttgccacaa acaggctgat accggtcagc agtgcgtgca
     3121 ggaggtacag cagcggtgca aggaacatga acaggaattc cagcggctca gtcacaccgg
     3181 tcaggaacgc agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt
     3241 tcggtgctgc gaagtacatc gccagcgccg cacccggcag accgaacatc ataatcggga
     3301 agaagccgga catgaacatc cccgcggtgc cgtcaccggc atagaagcgg ttgatatcac
     3361 cgtggaaaac cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt
     3421 tcagcacctg atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg
     3481 aacccagcgc gcccgcagaa acgatccact cgcctcctgc atggatagcg tgctgtaccg
     3541 gcggccagac gtaaccaaaa atggccgcca gcaccaggca gaagaagccg gtggcaatcg
     3601 gcacaaagcg tttgccgccg aagaaactca ggaagtccgg cagtttaata tcggaccaac
     3661 ggttataggc tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa
     3721 tttctgggtt gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca
     3781 gcgccgccgc acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta
     3841 atgcgaggtt atcaaaaatc gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat
     3901 ctggctgacc gaatcgcagc aacagtgccg ccaccggcag caccgcgata gggagctgta
     3961 acgccctacc gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac
     4021 cctatttggc tcgtttaaag ccgtattttt attttgctgc aaattgtact gccgatgttc
     4081 tgtaatcaga ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa
     4141 attaaacgcg tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta
     4201 ctggctaaac cagaaaactt attttatcat tcaaaaaatc aggtcggatt gacgcctgtc
     4261 tgcgcaaatc caggttacgc ttaaagatgc ctaatccgcc aacggcttac attttactta
     4321 ttgaggtgaa taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct
     4381 gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccga ctgccgatcg tccgtttgta
     4441 ctgggcctgc cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat
     4501 aaagcaggcc aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt
     4561 ctgccgaaag agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac
     4621 gttgatattc cagcagaaaa catcaacctt ctcaacggca acgccccgga tatcgacgcc
     4681 gaatgccgcc agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc
     4741 ggtgtaggta acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt
     4801 actcgtatca aaaccttgac tcatgacact cgcgtcgcga actctcgttt ctttgataac
     4861 gatgtcaatc aggtgccaaa atatgccctg actgtcggtg ttggtacatt gctggatgcc
     4921 gaagaagtga tgattctggt gctgggtagc cagaaagcac tggcactgca ggctgcagtt
     4981 gaaggttgcg tgaaccacat gtggaccatc agctgtctgc aactgcatcc aaaagcgatc
     5041 atggtgtgcg atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat
     5101 gaattagaag cagaaaatat caaaggtctg taattgttat ccctgccctg gctccttgct
     5161 cagggcaata ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct
     5221 ttaccggcca cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa
     5281 gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca
     5341 ttctctcccc cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg
     5401 acaccgctga agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat
     5461 caggctgtac taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg
     5521 gcgtgcgcgt tatgcgcgag tacctggcaa gacatccgaa tcaggcgtta ggtctgcatc
     5581 tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta
     5641 agcctgatgc cgcgctggtc gatttcctgt gtaagaacgc cgacgtcatt accaaagtga
     5701 ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg
     5761 tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc gaaagccggt ttccgcgcgg
     5821 ggattacctt tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg
     5881 gcctggcggg cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc
     5941 tgcatgttga ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc
     6001 tggttaccga cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta
     6061 aaacaatata ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat
     6121 ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg
     6181 aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat tggcgttgag aaacgtctcg
     6241 gcacactcgc cgcaggtaaa gtagccaacc tgaccgcatt cacgcctgat tttaaaatca
     6301 ccaagaccat cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg
     6361 cggacaagct cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta
     6421 tcgcctgatt gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct
     6481 tgcccccgcc agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga
     6541 agttgatcag caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg
     6601 caatttccac gcaatcggcg tacggcttgg tcgtcacgac gccaccatca ccctgtttga
     6661 tctcagcagc aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcaaac
     6721 gctggaacat gccctgttga atgccattgc tcagtttatt gatagctacc agcgcaaact
     6781 gcgcgagctg atcgcgattt cggtgatcct gccagggctt gttgacccgg acagcggcaa
     6841 aattcattac atgccgcata ttcaggtaga aaactggggg ctggtagaag ctctggaaga
     6901 acgttttaaa gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca
     6961 ctacttcggt gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac
     7021 cggggccggg attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga
     7081 aattggccat attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg
     7141 cctggaaact atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca
     7201 gggctaccag agccgcgtgc cgctggacga ctgcaccatc aaaactatct gcaaagccgc
     7261 gaacaaaggc gatagtctgg cgtcggaagt aattgagtat gtcggtcgtc atctgggtaa
     7321 aaccatcgcc attgctatca acttatttaa tccgcaaaaa attgttattg ccggtgaaat
     7381 caccgaagcc gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaggcgct
     7441 gaaggcgttt cgcactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat
     7501 cggcgctttt gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct
     7561 ggaaaattaa tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg
     7621 accattaaaa atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta
     7681 ccgggtgcag cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg
     7741 accaactatc cttcgcagac tgggcaagat ctggcgaacc gctttgccac cgcaggtgtc
     7801 gatgtacctg acagcgtgtt ttatacctct gcgatggcga ctgccgattt cctgcgtcgc
     7861 caggaaggca agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa
     7921 gccggtttca ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacgcgttcc
     7981 tacaactggg acatgatgca taaagcagcc tatttcgtcg ctaacggtgc acgttttatc
     8041 gccaccaatc cggacaccca cgggcgcggt ttttatcccg cttgtggcgc gttgtgtgca
     8101 gggattgaga aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc
     8161 atccgcgcag cattaaacaa aatgcaggcg cattcggaag aaacggtgat tgtcggcgat
     8221 aacctgcgta ccgatattct ggccggcttc caggcaggtc tggagacgat tctggtgctt
     8281 tctggtgttt cgtcgctcga cgatatcgac agtatgcctt tccgccccag ctggatttac
     8341 ccgtcagtcg ctgaaatcga cgttatctga atagcaacca cgtctcagga cgtggttggc
     8401 tctgatgaca aacgccaaac tgcctgatgc gctacgctta tcaggcctac gcagctcctg
     8461 caatatattg aattttcatg cttttgtagg ccggataagg cgttcacgcc gcatccggca
     8521 tgaacaaatc gcactttgtc agcaatttgg cccccgtaaa atataaacat tcaataaaac
     8581 ccgctaaaaa ttatcgattc atcaataaat atatcctcac accagacctt ttttcaccaa
     8641 tcgacaatca ccattacgtt ttttattttt tcaccgccaa atcgcttgcg cacagtgctg
     8701 ctttgcggca tttttttaaa caagcaaaca caacaagcaa caaataccag gttaacggag
     8761 aaggttatgt gttcaatttt tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt
     8821 aagaaagccc tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggtatt
     8881 tatgccagcg ataacgccat tctcgcccac gaacgtctgt caattgttga cgttaacgcg
     8941 ggggcgcaac ctctctacaa ccaacaaaaa acccacgtac tggcggtaaa cggtgaaatc
     9001 tacaaccacc aggcattgcg cgccgaatat ggcgatcgtt accagttcca gaccgggtct
     9061 gactgtgaag tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg
     9121 cagggcatgt ttgcctttgc actgtacgac agcgaaaaag atgcctacct gattggtcgc
     9181 gaccatctgg ggatcatctc actgtatatg gggtatgacg aacacggtca gctgtatgtg
     9241 gcctcagaaa tgaaagcgct ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg
     9301 agctatttgt ggagccagga cggcgaaatc cgttcttact atcatcgcga ctggttcgac
     9361 tacgatgcgg tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat
     9421 tcagttaaaa gccatctgat gtctgatgtg ccttacggtg tgctgctttc tggtggtctg
     9481 gattcctcaa ttatttccgc tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag
     9541 gaacgctctg aagcctggtg gccgcagtta cactcctttg ctgtaggtct gccgggttca
     9601 ccggatctga aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt
     9661 cacttcactg tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact
     9721 tatgatgtga ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag
     9781 gcgatgggca ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt cggcggttat
     9841 ctttacttcc acaaagcacc gaatgccaaa gaactgcatg aagagacggt gcgtaaactg
     9901 ctggccctgc atatgtatga ctgcgcgcgt gccaacaaag cgatgtcagc ctggggcgtg
     9961 gaagcacgcg ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg tattaaccca
    10021 caggataaaa tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgaa
    10081 gcgtatctgc ctgcaagcgt ggcctggcgg cagaaagagc agttctccga tggcgtcggt
    10141 tacagttgga tcgacaccct gaaagaagtg gctgcgcagc aggtttctga tcagcaactg
    10201 gaaactgccc gcttccgctt cccgtacaac acgccaacct ctaaagaagc gtacttgtat
    10261 cgggagatct ttgaagaact attcccgctt ccgagcgccg ctgagtgcgt gccgggcggt
    10321 ccttccgtcg cttgttcttc cgctaaagcg atcgaatggg atgaagcgtt caagaaaatg
    10381 gacgatccgt ctggtcgcgc ggttggtgtt caccagtcgg cgtataagta agaaatgcga
    10441 aacagccccg gtgaaatccg gggctgtttc agttattgag gtgcttgttg attctgatat
    10501 gcagtaagga aatgttccgg gtttgccaca tcactgtttg acaactcatc actgtaggcc
    10561 agtatttcat agattaattt cccaccacta ttgttaaatt ccgaatttca tcctgtatga
    10621 caaaagaatg gctgtagggt gggaaaaaac acaaagtaaa caataattga cgaatatagc
    10681 gccacgctgt tcgcaaccta accaaacagt cactttcgag caattttcct tgaaaaagag
    10741 gttgacgctg caaggctcta tacgcataat gcgccccgca acgccgataa ggtatcgcga
    10801 aaaaaaagat ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca
    10861 caggttcgaa tcccgtcgta gccaccatct tttttgcggg agtggcgaaa ttggtagacg
    10921 caccagattt aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc tcccgcacca
    10981 ttcaccagaa agcgttgtac ggatggggta tcgccaagcg gtaaggcacc ggtttttgat
    11041 accggcattc cctggttcga atccaggtac cccagccatc ttcttcgagt aagcggttca
    11101 ccgcccggtt attggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    11161 ctggttcgaa tccaggtacc ccagccatcg aagaaacaat ctggctacgt agctcagttg
    11221 gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccaa
    11281 attctgaatg tgtcgaatat gttcggcaaa ttcaaaacca atttgttggg gtatcgccaa
    11341 gcggtaaggc accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc
    11401 aatttattca agacgcttac cttgtaagtg cacccagttg gggtatcgcc aagcggtaag
    11461 gcaccggatt ctgattccgg cattccgagg ttcgaatcct cgtaccccag ccacattaaa
    11521 aaagctcgct tcggcgagct ttttgctttt ctgcgttcat tcaatgtcga atgcgatgtt
    11581 gacacgtctt atccttcaat gtcggatgcg acgctgccgc gtcttatccg acctacggtt
    11641 ggcacgcatc cggcaatgtt gtaaggctac aaccctaacg catatttcag cgcctgacgt
    11701 ttcaacacgc cagcacgctc cgccgccatt aacccgagat tacgcataaa acgcagtggt
    11761 ggcagattat tgctgaatcc ggcataaaac agatccatac cgctttgcat aatgaagtta
    11821 tccgccatgc gccgcatctg gtaacgcttg aggacaggat aactggccca cgcttcgccg
    11881 tagctgcggg cgttgaccag aacatcaatc agggcatcga catcacgata accaagattc
    11941 actccctgcc ccgccagcgg atggatggta tgcgcggcat cgcccaccag cgcaagccct
    12001 ggctgcacgt actgcaacgc atggcgacgc gtcagcggaa acgcaccagc ggcaagcggt
    12061 gtaacgtaac ccagacgcga cgggaaatgc ttcgcgattt ccgcctggag ctgtgccata
    12121 ttcatattct gcaactggcg aatacgcgcc ggagagtcat accacaccag cgatgcccag
    12181 ttatcaaaca acggcagaaa cgcacgcggt ccgtccggag taaattgctg ccaggtgctg
    12241 tcgccgggat cgttctcgca ctggacgcta atcaacatgc acgactgcgc atactgccat
    12301 gcatgaacgc caattcccgc catctgccgc acctgcgaat ttgcgccgtc ggcaccaatc
    12361 accagcttcg cgcgaatcac ttcaccgcct ttcagctcca gctcctgaag atcatcatgg
    12421 cgatgcagcg caatcagcga gcctggcaca cgtaacgtta ctttcggatg cgcttccagc
    12481 gcctgccaca acgcctgttg caggacagtg ttttccacca tatagccaag cagcggtagc
    12541 ttaagttcag cggcgtcaaa caccacatgc gccgtttccc actcccacgt ttccagtctg
    12601 cggtaaggat ggcaacgcat agcctgtact gcatcccaga cccctaaccc tttaagcaat
    12661 gataccgaag ccgcgctgat cgccgagatc cgcacgtccg gttggctatc agcgacaaac
    12721 ggcgctggtt ctgcgtgctc gatcaccgtt accgcaaatc cgtgctgtgc cagccccagc
    12781 gccagtgcgc cgccgaccat tcctccgccg acaatggcaa tttccgttgg ttgatttgtc
    12841 atggtcaatc atcctgttag caatatgctt taagtttaca ggatttttac ccctaagggc
    12901 tgataacgct catactggtc acaacggcag caaagcatta cactatgcgc cctgcattcc
    12961 tggctactat ttcgcaagag caagtcgatg accaaaaaac tccatattaa aacctggggc
    13021 tgtcagatga acgagtacga ttcatcgaag atggccgatc tgctggatgc cacccacggc
    13081 tatcaactga ccgacgtggc ggaagaagcg gatgtgctgc tgctgaacac ctgctcaatc
    13141 cgcgagaagg ctcaggaaaa agtcttccat cagttgggtc gctggaaact gttaaaagag
    13201 aagaatccag acctgattat cggcgtcggt ggctgcgtgg catcgcaaga aggcgagcac
    13261 attcgccagc gcgcccacta tgtcgatatt atttttgggc cgcaaacgct gcaccgtctg
    13321 ccggagatga tcaactccgt gcgtggcgac cgcagcccgg ttgtagatat cagcttcccg
    13381 gaaatcgaga agtttgaccg tctaccggaa ccgcgcgccg aagggccgac cgcgtttgtc
    13441 tccatcatgg aaggctgcaa taaatattgc acctactgcg tggtgcctta cacccgtggt
    13501 gaagaggtaa gccgtccgtc cgacgatatt ctgtttgaga ttgcccagct tgcggctcag
    13561 ggcgtgcgtg aagtcaacct gctcggtcag aacgtgaacg cctggcgtgg tgaaaactac
    13621 gacggcacca ccggatcgtt tgccgatctg ttgcgtctgg ttgctgcgat cgacgggatc
    13681 gatcgtattc gctttaccac cagccatccg atcgaattca ccgacgatat catcgaagtg
    13741 taccgcgaca cgccggagct ggtgagcttc ctgcacctgc cggtacagag tggttccgat
    13801 cgcattctga acctgatggg ccgtacccat acggcgctgg agtacaaagc gatcatccgt
    13861 aaactgcgtg cggcgcgccc ggatattcag atcagttctg acttcatcgt cggcttccct
    13921 ggcgaaacca ccgaagactt cgagaaaacg atgaagctga ttgccgacgt caatttcgac
    13981 atgagctaca gctttatctt ctctgcacgt ccgggtacac cagccgccga tatggttgat
    14041 gatgttccgg aagaagagaa gaagcagcgt ctgtatattc tgcaagaacg tatcaatcag
    14101 caagcgatgg cgtggagccg ccggatgctc ggcaccaccc agcgtattct ggtagaaggt
    14161 acatcgcgta agagcatcat ggagctttcc gggcgtaccg aaaataaccg cgtggtcaac
    14221 ttcgaaggta cgccggatat gatcggtaaa ttcgtcgatg tagaaattac cgacgtctac
    14281 ccgaactctc tgcgcggtaa agtggtgcgt actgaagatg aaatgggtct gcgcgtggca
    14341 gaaacaccgg aatcagtgat tgcccgtact cgcaaagaaa acgaccttgg cgtgggttat
    14401 tatcaaccgt aattctcagg cccgccgttc cggtgggcct tgttttttct ctctttatcc
    14461 ccatctttat cgcaatgctt gcgccttttt cctgtggcgt gaataatttc cttataggcc
    14521 ggtgaatgtt cagcttccgg cggcatacag ataagaggaa cggtttgaac atagacactc
    14581 gcgaaatcac cctggagcca gcagacaatg cgcgtctgtt gagcctgtgc ggcccgtttg
    14641 atgacaacat caagcagctc gaacgccgtc tcggcatcga gatcaatcgc cgcgataacc
    14701 actttaaact gaccggccgt ccgatttgcg tcaccgctgc ggcagacatt ctgcgtagcc
    14761 tgtatgtcga tactgccccg atgcgcggtc agattcagga tatcgaaccg gaacagatcc
    14821 accttgctat taaagaagca cgggtactgg agcaaagcgc ggagagcgtg ccggagtacg
    14881 gcaaagcggt caatatcaaa accaaacgcg gcgtaattaa gccgcgcacg ccaaaccagg
    14941 cgcagtacat cgccaatatt ctcgaccatg acattacctt cggcgttggc ccggcgggta
    15001 cgggtaaaac ctacctggca gtggctgcag cagttgatgc cctggagcgt caggaaattc
    15061 gccgtattct gctgactcgt ccggcggtcg aagccggtga gaaactgggc ttcctgcctg
    15121 gcgatttaag ccagaaagta gacccgtatc tgcgcccact gtacgacgcg ctgtttgaaa
    15181 tgctgggctt tgagaaagtc gagaaactga ttgagcgcaa cgttattgaa gtcgcgccgc
    15241 tggcctatat gcgtggtcgt acgctgaacg acgcgtttat cattctcgat gagagccaga
    15301 acaccaccat cgaacagatg aagatgttcc tgacccgtat cggttttaac tcaaaagcgg
    15361 ttatcaccgg cgacgtcacg cagatcgacc tgccgcgtaa tactaaatca ggcttacgtc
    15421 atgccatcga agtgctggcc gatgtcgaag agatcagctt taacttcttc cacagcgaag
    15481 acgtggttcg tcacccggtg gtggcgcgta tcgttaacgc ctatgaagcc tgggaagaag
    15541 ccgaacaaaa acgtaaagcg gcgctggcgg cagaacgcaa gcgcgaagaa caggaacaaa
    15601 aatgagtcag gtgatcctcg atttacaact ggcatgtgaa gataactccg gattgccgga
    15661 agagagccag tttcaaacat ggctgaatgc ggtgatcccg cagtttcagg aagagtcgga
    15721 agtgacgatt cgcgtggtcg ataccgccga aagccacagt ctcaatctga cctatcgcgg
    15781 taaggataag ccgaccaacg tgctctcctt cccgtttgaa gtgccgcctg gcatggagat
    15841 gtcgctactg ggcgatctgg ttatctgccg tcaggtggtt gagaaggaag ctcaggagca
    15901 aggcaaacca ctggaggcac actgggcgca tatggtggtg cacggcagtc tgcatttgtt
    15961 aggttacgat cacatcgaag atgacgaagc agaagaaatg gaagccctcg aaacagagat
    16021 tatgcttgct ctgggctatg aggatccgta cattgccgag aaagaataat ttgccaggca
    16081 tttgactggc agacgtcccc gccgttggtg attaacgacg gcgttttgat taactaacat
    16141 gagaacccat acgacgccat gagcgacgac aattcacaca gtagtgacac gataagcaac
    16201 aagaagggat ttttctccct gttactcagc caacttttcc acggtgaacc gaaaaaccgt
    16261 gacgaactgc tggcgctgat ccgtgattcc gggcagaacg accttatcga cgaagatacg
    16321 cgcgatatgc tcgaaggggt gatggacatc gcagaccaac gcgttcgcga catcatgatc
    16381 ccccgctccc agatgattac cctgaaacgc aaccagacgc tggacgaatg ccttgatgtc
    16441 atcatcgagt ccgcccactc acgtttcccg gtgattagcg aagacaaaga tcacattgaa
    16501 gggattctga tggcgaaaga cttgctgccg tttatgcgca gcgatgctga agccttcagc
    16561 atggacaaag tgttacgtca ggcggttgtc gttcctgaaa gtaagcgcgt agaccgaatg
    16621 ctgaaagagt ttcgctctca gcgttaccac atggcgatcg ttattgacga attcggtggg
    16681 gtttccggtc tggtgaccat tgaagacatc ctggaactga ttgttggtga gattgaagac
    16741 gagtatgacg aagaagatga tatcgacttc cgtcagctga gtcgtcatac ctggaccgtg
    16801 cgcgcactgg cttccattga agacttcaac gaagcgttcg gcacccactt tagcgatgaa
    16861 gaagtcgaca ctatcggtgg tctggtgatg caggcatttg ggcatcttcc ggcacgtggc
    16921 gaaaccatcg acatcgacgg ttaccagttc aaagtggcga tggccgatag tcggcgtatt
    16981 attcaggttc atgtcaaaat cccggatgac tcaccccagc caaagctgga tgaataaaac
    17041 cgaaactgga tagataacta catggctttt gcctcattaa ttgaacgcca gcgcattcgc
    17101 ctgctgctgg cgttattatt cggtgcctgc ggaacgctgg ccttctctcc ttacgacgtc
    17161 tggcctgcgg cgattatttc gttgatgggg cttcaggcgt tgacctttaa ccgccgtcca
    17221 ctccagtctg ccgctattgg cttttgctgg ggatttggcc tctttggcag cggtattaac
    17281 tgggtctatg tcagcatcgc gacctttggc ggaatgcctg gcccggttaa catcttcctg
    17341 gtggtgctgc tggcggcgta tttgtcgctg tataccggac tgtttgctgg cgtgctgtcg
    17401 cgtctgtggc cgaaaaccac ctggctgcgc gtggcgattg ccgcccctgc tctctggcaa
    17461 gtgaccgagt ttctgcgcgg ttgggtactg acaggcttcc cgtggttaca gttcggctat
    17521 agccagattg atggcccgtt aaaagggctg gcaccgataa tgggcgtgga agccattaac
    17581 ttcctgctga tgatggttag tggcctgctg gcactggcgt tggtcaaacg caactggcgt
    17641 ccgctggtgg tggccgtcgt gctgtttgcc ctacccttcc cgctgcgtta catccaatgg
    17701 tttaccccgc aaccggagaa aaccattcag gtttcgatgg ttcagggcga tattccgcaa
    17761 tcactgaaat gggatggaga tcagcttctt aatacgctga agatttacta caacgcaacg
    17821 gctccgctga tgggcaaatc atcgttgatt atctggccgg agtcggcgat aaccgatctg
    17881 gaaattaatc agcaaccgtt cctcaaagca ctggacggtg agttgcgcga taaaggtagc
    17941 tcgctggtaa ccgggattgt cgacgcgcgt ctcaataagc agaaccgcta cgatacctac
    18001 aacaccatca tcacactggg taaaggtgca ccgtacagct acgaatcagc cgatcgctat
    18061 aacaaaaacc atctggtgcc gtttggcgag tttgtcccgc tggagtcgat tctgcgtccg
    18121 ttagcaccgt tctttgatct gccgatgtcg tcgttcagcc gtgggccata tatccagccg
    18181 ccgctgtcgg taaatggtat tgagcttact gcggccattt gctacgagat cattcttggc
    18241 gagcaagtgc gcgataactt ccgcccggat accgactatc tgctgactat ctccaacgat
    18301 gcgtggtttg gtaaatctat tggtccatgg caacacttcc agatggcgcg aatgcgtgcg
    18361 ctggagctgg cgcgcccact gttgcgcagc accaacaacg gcattacggc ggtgattggc
    18421 ccgcagggtg agattcaggc gatgatcccg cagttcaccc gcgaggtgtt gaccactaac
    18481 gtgacgccga cgaccggact cacaccgtac gcacgaaccg gcaactggcc gctgtgggtg
    18541 ctgacggcgc tgtttggttt tgccgctgtg ctgatgagtc tgcgagcacg taaacgttga
    18601 tgtgcctgat gtatccggca tgagcaaaac gtactttctc attaagcttt ggggcatggt
    18661 tatcatgccc ctttttctta cggttcaggg atacaagcca cattgccata ttggggatga
    18721 cgaataattg cctgcggatc aagcgagcgc gtcctgtcca gttggattaa cccgttcttc
    18781 gccataacaa atatctttct accatccgcc gaaaaacgaa ggtgattaac gtagctggca
    18841 ggataaacgc caacaatctg cccggcatta atattgagca ggatgacata accttgcaag
    18901 ccatatgaca ccaacgctaa atactgaccg tccgggctaa taccctcaac ggaataaacg
    18961 ctcctgctac accattgcgt cataatgctg gcttgctgat ccccaactct tatacgtgaa
    19021 atcccctggc tgtcggcaaa aattacatcc ccgttttcag gtcgaaaacg catcgccgat
    19081 atcgttccac gcgcatcacc tgcgggaaag tcttctttct gatgcgtgat ggtattgatg
    19141 cgtacaatgt cacctctgac cataccgggt atcaacaata ttttttcatc cggggaaagt
    19201 gccataaccc aggcaccagg tttattactc gtccccgtcc cgtcgtattt ctccagcggg
    19261 atatccgcca catgctgata agtcaccaga tcaatcgctg acacactatt actggtgcga
    19321 ttcatcaccc aggcttttgt gccatcacgc gagaggataa gactgtcagg atgtgcaccg
    19381 gtggtaatcg actgaataat ctcccccgtc gccacgttaa tgaccgataa cgcattcgct
    19441 tcaaatgaag tcgcataaag caacgtttca tccgggctta gcgtcagcga tctgacgccc
    19501 tgatctttac agccaccaca atgtttgact gacttttctt tcgctatggg ggctgtaaag
    19561 gtatgcagga ttttcttttg tccgaggtcg aaaacaacaa tgctgttggt gttttcaatt
    19621 gcggcataca acgtattacc atcccgactg atcgccatat catggatatc ggcaatctca
    19681 gcgagcaaat caccactggc ctgttcacga cttaccggag cggataatgt ttccctgagg
    19741 gcgttgcgtt cctccgctaa ctgttgtttt ttttgtcgtc gctcttgttc aatctcctgc
    19801 ggataaaact cttcacgcag gctttgcctg acctgtaaaa ctgactcatc catctgctgc
    19861 tgcgcaagcc agatagcccc ggtcagaaga agactgcccg caacaataat cccgccttcc
    19921 gtaacgagtc ggttttgccc agataaacgc gcgctcagcc aacccaacgc gccacccgca
    19981 atcaggccgc caatgtggca agcgttatct attcctgttt gccgggcacc gtacaacagt
    20041 gtcagcgcca ccatacccaa cagaggaaat actcgccgct ggtttttatg gtgtgtgccc
    20101 gtacctgaag caagctgtgt tgctatcgca gcgccagcta ttcccataat tgccccggat
    20161 gccccaacgc tgatgactat ctgatcacta tcgcgtaacg cataacaggc gctgatgagc
    20221 ccaccaaaga cgccggagaa taaccaaata atcagaagtc gccatttccc cagtatccgt
    20281 tccgtcactg cccccaccac caggagtgca aacatattca tcagcaaatg ggaaaaacta
    20341 ctgtgcagaa agacactgct caataagcgc cagggttcac ccgacaacga gagtgaagca
    20401 acgttagcac cgagatggat gagtgcggat tgatcgactt cttcataact catccattgt
    20461 gcgaggtaac aaaatatccc aacacagagc gcaaaaagga taagggttat tgcgggtaat
    20521 tgaacattta acggcttaac cgaagatgca gacataaggt gtttcttcct tgaaaagaca
    20581 gataaaaagc atttttcccg tgtgcagtta tcatcccggc aaacgtccca taccgaaaag
    20641 ttaaccctgt ccgaaatatg actttttcag cctaaaatta caatcgtaat taattcgccc
    20701 ctaagtattt tttggcatgt aagttgcttt atttactgtg taagcgatga aatcagccgc
    20761 gacgcaacca ggttgcacca ccacaaatca gcggtagcac caaaagagtg cattttaaga
    20821 attttgcttc ggaatggtgc ggcgcatctc gcaaaaacaa acaataaaac agcaatatct
    20881 ttacatttag ccaaactaaa tgttacctaa caggcacaac actgcacaat aaagttgcag
    20941 acgataacaa cacaaacact cacaacgggt atccatgcgt tcttaacgca gaagataaag
    21001 gagttggata tgcaattacg taaacctgcc atagcaatcc tggccctggc gctttccgca
    21061 ggactggcac aggcagatga cgccgccccg gcagcgggca gtacgctgga caaaatcgcc
    21121 aaaaacggtg tgattgtcgt cggtcaccgt gaatcttcag tgcctttctc ttattacgac
    21181 aatcagcaaa aagtggtggg ttactcgcag gattactcca acgccattgt tgaagcagtg
    21241 aaaaagaaac tcaacaaacc ggacttacag gtgaaactga ttccgattac ctcacaaaac
    21301 cgtattcctc tgctgcaaaa cggcactttc gattttgaat gtggttccac caccaataac
    21361 gtcgaacgcc aaaaacaggc ggctttctct gacactattt tcgtggtcgg tacgcgcctg
    21421 ttgaccaaaa agggtggcga tatcaaagat tttgccgatc tgaaaggcaa agccgtagtc
    21481 gttacttccg gcactacctc tgaagttttg ctcaacaaac tgaatgaaga gcaaaaaatg
    21541 aatatgcgca tcatcagcgc caaagatcac ggtgactctt tccgtaccct ggaaagcggt
    21601 cgtgccgttg cctttatgat ggatgacgcc ctgctggccg gtgaacgtgc gaaagcgaag
    21661 aaaccagaca actgggatat cgtcggcaag ccgcagtctc aggaagccta cggttgcatg
    21721 ttgcgtaaag atgatccgca gttcaaaaag ctgatggatg acaccatcgc tcaggtgcag
    21781 acctccggtg aagcggaaaa atggtttgat aaatggttca aaaatcccat tccgccgaaa
    21841 aacctgaaca tgaatttcga actgtcagac gaaatgaaag cactgttcaa agaaccgaat
    21901 gacaaggcac tgaactaatt acaagaacca ggggcggaaa ttccagccct ctcgattgtt
    21961 acgtagcacg gacagactat acgcctgatg gtcgttcccc atcgggcctg aaaaccgcaa
    22021 tacgctgggt aacaatcttc gagggtagca gttaacgctg ctaccctctt ttttctggag
    22081 tagatttatg tctatagact ggaactgggg tattttttta caacaagccc cgttcggcaa
    22141 caccacctat cttggttgga tctggagtgg ttttcaggtg acgatcgctt tgtcgatctg
    22201 cgcctggatt atcgctttcc tcgtcggttc atttttcggc attttacgta ccgttccaaa
    22261 ccgttttctc tccggtctgg gtacgttgta tgtcgaactg ttccgcaacg tgccgttgat
    22321 tgtgcaattc tttacctggt atctggtgat cccggagctg ctgccggaga aaatcggcat
    22381 gtggtttaag gccgagctgg atcccaatat tcagtttttc ctttcatcca tgctctgcct
    22441 ggggctgttt accgccgccc gtgtttgcga acaggtacgc gccgctattc agtcgctgcc
    22501 gcgtgggcag aagaatgccg cgctggcgat gggtctgacg ctaccgcagg cttaccgcta
    22561 cgtgctgttg cctaacgcgt atcgcgttat cgtcccgcca atgacttccg agatgatgaa
    22621 cctggtgaaa aactccgcca tcgcctcgac tatcggtctg gtggatatgg cagcgcaggc
    22681 gggtaaattg ctcgactact cggcccacgc ctgggagtcg tttaccgcca ttacgcttgc
    22741 gtatgtgttg attaacgctt ttatcatgct ggtgatgacg ctggttgaac gtaaagttcg
    22801 cctgcccggc aacatggggg gcaaataatg tacgagtttg actggagttc cattgttcct
    22861 tccctgccat atctgctcga cgggctggtg atcaccctga aaatcaccgt cacggcagtc
    22921 gtgattggta ttttgtgggg taccatgctg gcggtgatgc gcttgtccag cttcgcgcct
    22981 gtttcgtggt ttgccaaagc ctacgttaac gtattccgct cgattccttt agtcatggtt
    23041 ttgctgtggt tttacttgat tgtgccggga tttctgcaaa atgtgctggg attatcgcca
    23101 aaaaatgata ttcgtttaat ttcggcgatg gtggcgtttt cgatgtttga agcggcctac
    23161 tattcagaga ttatccgtgc cggtattcaa agtatttctc gtggtcagtc gagcgccgcg
    23221 ctggcgttgg gaatgactca ctggcagtcg atgaaactga ttattctgcc gcaggcgttc
    23281 cgcgcgatgg tgccgctgct gctcactcag ggcatcgtac tgttccagga tacctcgctg
    23341 gtgtatgtgt tatctctggc cgatttcttc cgtaccgcct caaccattgg tgagcgtgat
    23401 ggtacgcagg ttgagatgat cctgtttgcc ggatttgttt atttcgttat tagtcttagc
    23461 gcgtcgttgt tggtcagcta cttgaaaaga aggacagcat aatgattacc ctgaaaaatg
    23521 tttcaaaatg gtatggtcac tttcaggtgc tgaccgactg ctcaaccgaa gtgaaaaaag
    23581 gcgaagtggt ggtggtttgc ggcccgtctg gttccggcaa atcaacgctg attaaaaccg
    23641 tcaacggcct cgaaccagtg cagcaaggtg aaatcaccgt cgatggtatc gtggttaatg
    23701 acaagaaaac cgatctggca aagctgcgtt cccgcgtcgg gatggtgttc cagcattttg
    23761 agttgttccc ccatttgtcg attattgaaa acctgactct ggcgcaggtg aaagtgctta
    23821 aacgcgataa agcgcccgct cgtgaaaaag cattgaaatt gctggagcgc gtgggacttt
    23881 ccgcacatgc caataaattt ccggcgcagc tttccggcgg tcagcagcag cgtgtggcga
    23941 tcgctcgcgc attgtgtatg gatcctattg ccatgctgtt cgacgaaccg acatcggcgc
    24001 tggatccgga gatgatcaac gaagtgctgg acgtgatggt ggaactggca aacgaaggaa
    24061 tgaccatgat ggtggtgacc cacgaaatgg gttttgcccg taaagtggcg aatcgggtga
    24121 tctttatgga tgagggtaaa attgtcgaag actcgccgaa agatgcgttc ttcgatgatc
    24181 cgaaatcgga ccgcgcaaaa gacttcctcg cgaaaatcct gcattaatca ctctggcgcg
    24241 catacccaat tgcgcgccat ctcacgcttt atcgtttcgc ccctcgtatc tgttacatcc
    24301 tggcgttacc cttgtcgcaa agaagcacac aacaaggagc aacaatggca ctgccaattc
    24361 tgttagattg cgacccaggt catgacgacg ctatcgcaat agttctcgcc ctcgcctcac
    24421 cagagcttga tgtcaaagca attacgtctt ccgccggaaa ccagacacca gaaaaaacct
    24481 tacgcaatgt tcttcgtatg ctgaccttgc tcaatcgcac cgatattccg gtagcaggcg
    24541 gcgcggtaaa accgttaatg cgtgagttga ttatcgcgga caatgtgcac ggcgaaagcg
    24601 gtctcgacgg cccggcatta ccggaaccgg cgttcgcacc gcaaaactgt acggtggtag
    24661 agctaatggc gaaaacgctg cgtgaaagtg cggaacctgt caccattgtg tctaccggac
    24721 cgcaaactaa cgttgccttg ctgctcaata gccatccgga actgcatagc aaaattgccc
    24781 gtatcgtgat tatgggcggc gcaatggggc ttggtaactg gacgccagcg gctgaattta
    24841 atatttacgt tgacccggaa gcggcagaaa ttgtcttcca gtcagggatc ccggtggtga
    24901 tggccggtct ggatgttact cataaagcac aaatccacgt tgaagacacc gagcgtttcc
    24961 gcgcgattgg taaccctgtt tcaaccattg ttgccgaact gctggatttc ttcctcgaat
    25021 atcataaaga cgaaaaatgg ggctttgtcg gcgcaccact gcatgaccca tgcaccatcg
    25081 cctggctgtt gaaaccggag ttatttacct ctgttgagcg ctgggttggc gtggaaacac
    25141 aggggaaata tacccagggt atgacggttg ttgattatta ttatctgaca ggcaataaac
    25201 cgaatgccac cgtaatggtc gatgttgatc gtcagggctt tgttgattta ctggctgatc
    25261 gtctgaaatt ttacgcttaa cacacgtcat tgattaagat caccacgcgt tacctgggtg
    25321 gtcttaatct gtttatctct ttttctttca agatataaat gcaatggata atgcagaact
    25381 cgccattggt atcgatctcg gtacgaccaa tagtttaatt gccgtctgga aagacggtgc
    25441 cgcgcaatta attccaaata agttcggtga atatttaaca ccatccataa ttagcatgga
    25501 tgaaaataat catattttag tcggaaaacc ggctgtatca cggcgtactt cgcatccgga
    25561 taaaacggca gcgttattta aacgtgcaat gggcagtaat accaactggc ggttaggcag
    25621 cgacacattt aacgcgccag aactgtcctc tttgatatta cgctcattaa aagaagatgc
    25681 cgaagaattt ctgcaacgtc cgattaaaga tgtggtgatc tccgttccgg cttatttcag
    25741 cgatgaacaa cgcaagcata cccgtttagc agcggagtta gccgggttaa atgcggtacg
    25801 cttaattaat gaacccacag cagctgcgat ggcgtatggc ctgcataccc aacaaaatac
    25861 ccgttcgctg gtgtttgatc tcggtggcgg cacgtttgac gttacggtgc ttgagtacgc
    25921 cacgccggtg attgaagttc acgcctccgc tggcgacaac tttcttggtg gcgaagattt
    25981 tacccatatg ctggtcgatg aggttttaaa acgcgcggat gtcgccagga ccacgcttaa
    26041 cgagagtgaa ctggcagcct tgtacgcctg tgtggaagcg gcaaaatgta gcaatcaatc
    26101 gccattgcac attcgctggc agtatcagga agaaacgcgg gaatgcgaat tttacgagaa
    26161 cgaactggaa gatttgtggt tgccgctgct caatcgcttg cgagtgccga ttgaacaggc
    26221 gttgcgcgat gcgcgtctga agccgagtca aatcgacagt ctggtgctgg ttggcggcgc
    26281 gtcacaaatg ccgctggtgc agcgaatcgc cgtgcgtctg tttggcaaat taccgtatca
    26341 aagttacgat ccgagcacca ttgtcgcgct gggcgcagca atccaggccg cctgccgctt
    26401 acgcagtgaa gatattgaag aggtaatcct cactgatatt tgcccttact cgttgggcgt
    26461 tgaagttaac cgccagggcg tttccggcat tttctcgccg attattgaac gaaacaccac
    26521 tgtgcccgtg tcgcgtgtag aaacttattc aaccatgcac ccggaacagg attcaattac
    26581 ggttaacgtc tatcagggag aaaaccacaa agttaaaaac aacattctgg tggaatcctt
    26641 cgatgtgccg ttgaagaaaa ccggggctta tcagtcgatt gatattcgct ttagttatga
    26701 tatcaacggg ttgcttgaag ttgacgtgct tctggaagac ggcagcgtta agtccagagt
    26761 gattaaccac agcccggtaa cattgagcgc gcagcagatt gaagagagtc ggacgcggtt
    26821 atccgcattg aaaatttatc cgcgcgatat gctcatcaat cgcaccttta aagccaaact
    26881 ggaagagttg tgggcgcggg cgcgggtgac gagcgagaag agatcggccg ggtgatcacc
    26941 gattttgatg cggcgttgca gtcaaacgat atggcccgcg tggatgaagt tcggcggcgg
    27001 gcgagcgatt atttagccat tgagatccca taaacacggc tgccggatgc ggcgtaaacg
    27061 ctgcatccgg catcggcgca aaaaacacat cgtcagctaa acaacatcgc gattttgccg
    27121 agagcataac ctatcaccag caccgttaaa ctgattttga taatgctggt aatgctctgt
    27181 ggtttttcac cctcatcatt tttttcttca tgattttcag gtgttggctc atcgacatct
    27241 tcatcctccg gctcgagttt cactttcgtc aaaagcatcc gggtgacaat ggcctctaat
    27301 ggatgagcat ctccgccttc cagccttgta ttaagacggg ccagcatctc ttgtttcgga
    27361 aacgcttcaa gtactacctg ttccgctacc cgatacaaat ccaggaacgg tgaatcttgc
    27421 tctgtctcgc taccgactct cgatttccac cacgcccatg ctttgcgctt gaagaataac
    27481 gactcaataa acgattgacc taacggcgat aattttccgc tatgtaaaat ctccgcccat
    27541 aacagccgcg tccgggcgct ctcaccagga acatatttca gcggttgacc ttgcgcatct
    27601 accatgctcc gcgcttcgat aaccatcact aataacgcga aataatccgg gcaatgacgc
    27661 ttgctccact gcaacaacaa attatcagct tgtgtagatg ggtaactttc ccagtacgcg
    27721 cagagatcgg caagcaggct ttccccctcg ccgcagtaca aacgttgtgc acacaagtaa
    27781 taccgggcat cttcgctttc tggttcttcc gtttgccact gttgagcgac aggaatgagt
    27841 tccgccatac ctgtttgcac cgagctgtac cagcgcaaca gtttgcgatg taactttgca
    27901 tcatcaggaa ttaaccacgg accttccatc gccaaaaacg cagtgacata gtcaggatgg
    27961 taacggcagc aacgttccag cgcgaagtaa tactcaacag tctggttctg caacgcaatg
    28021 ggatattcca gaaggctaag gaaatcgaaa caatcaccag cctgaatggc ctcaagaaaa
    28081 gcctcccttt cttcctcatc cattccttcg ctgtcattaa atgaatgcca gttcaaacgc
    28141 tctgccagca aagaagcaca atttaatgaa agatatcgcg cttctatggc gattgcacac
    28201 agcggccagc gtaattgatc gacatcctcc atgttccagg tatttaattg ctgaataaat
    28261 ttctgccagg cggaaggctg aaaacgatca ctttctgaat ccagtaatgt cctgaatgca
    28321 cgtagtattt catgttcagc ggcagcatct ttttcttcat catctgcttc ctcgacagga
    28381 tttaccgcca gtcgcagggc ctcttcatag gcctggcgta atgctttaaa cccttgcgga
    28441 tcggtttcgg gatgacacaa cggtaagcga gcaagataag cctggcggat aatgtctatt
    28501 tgcgtcgtgc tttcaatttc gaggatttgc caacatgttt tcataggtcg ctcacatcct
    28561 taagcgttac cgggcattca gactggacag ttaatggctg ccagggaagc gtggcataga
    28621 aacgatcgct tcccgctgcc agctcttcga acatacgact cccggcgcta tcgtatttgt
    28681 acagggttag cgcctcgcgt agcgaagtgt tgtcttcgca ctgagaagat tgccagtaga
    28741 gacgtcctaa cgaataggcg gaaaaatagt gcatccagct gtgatagtaa tggtgagctc
    28801 gtacatagac gcgcgattgc agccataaac tctcttcttc cgtcagccac ttgttgagca
    28861 caccgacccg tgacagaaac cccattcgaa cataatccca ggctttgatt cccccttccc
    28921 cacagcacat agcggtttgg gcaacgaatt gggtataaat caaccctctc tccgagccac
    28981 cagcaattaa tgctttccac tcttcagggg aagagcgatg ccatgtcaga tataagccag
    29041 ctaaatcatt ggcatgacca tcatcagtca tctgataaat agtctgaatt aaatcatcac
    29101 gattatttat tccccaggat gatgagagct cgaattcttt tatcccagta tacatttttg
    29161 gcgagtcatg cgcgggtttg caaccctgat taagtatttc catgggcgca gaaagcgcaa
    29221 aaagaagata ttgttcttcc ttattcattt gttaataatc ccttaagtgc attaactgac
    29281 tactttttta ttattgatga gtataaactt gacgctttca aaataaaaag aaa
//
LOCUS       DAJOSY010000053        28447 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJOSY010000053 DAJOSY010000000
VERSION     DAJOSY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.053"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            84..527
                     /gene="cmtB"
                     /locus_tag="OHN27_002409"
     CDS             84..527
                     /gene="cmtB"
                     /locus_tag="OHN27_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCQ2286830.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            555..1943
                     /gene="cmtA"
                     /locus_tag="OHN27_002410"
     CDS             555..1943
                     /gene="cmtA"
                     /locus_tag="OHN27_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCQ2286831.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1958..3235
                     /locus_tag="OHN27_002411"
     CDS             1958..3235
                     /locus_tag="OHN27_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCQ2286832.1"
                     /translation="MKTKVAAIYGKRDVRLRVFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3232..4197
                     /gene="yggF"
                     /locus_tag="OHN27_002412"
     CDS             3232..4197
                     /gene="yggF"
                     /locus_tag="OHN27_002412"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCQ2286833.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLTDNLRNVAKALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4219..4728
                     /gene="fumE"
                     /locus_tag="OHN27_002413"
     CDS             4219..4728
                     /gene="fumE"
                     /locus_tag="OHN27_002413"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCQ2286834.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHTILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4725..5438
                     /locus_tag="OHN27_002414"
     CDS             4725..5438
                     /locus_tag="OHN27_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCQ2286835.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLTENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WLELASFCDFSIFIHAPAQILRERLISRKIAGGLTRQVAEAFYARTDGPNVERVLMNS
                     RQANLIVEMTEEGRYHFTS"
     gene            5723..6742
                     /gene="epd"
                     /locus_tag="OHN27_002415"
     CDS             5723..6742
                     /gene="epd"
                     /locus_tag="OHN27_002415"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCQ2286836.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6792..7955
                     /gene="pgk"
                     /locus_tag="OHN27_002416"
     CDS             6792..7955
                     /gene="pgk"
                     /locus_tag="OHN27_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCQ2286837.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8170..9249
                     /gene="fbaA"
                     /locus_tag="OHN27_002417"
     CDS             8170..9249
                     /gene="fbaA"
                     /locus_tag="OHN27_002417"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCQ2286838.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            9607..10467
                     /gene="mscS"
                     /locus_tag="OHN27_002418"
     CDS             9607..10467
                     /gene="mscS"
                     /locus_tag="OHN27_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCQ2286839.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            10606..11241
                     /gene="argO"
                     /locus_tag="OHN27_002419"
     CDS             10606..11241
                     /gene="argO"
                     /locus_tag="OHN27_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCQ2286840.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VMKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLALLAAWLAPRLRTAKAQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            11334..12074
                     /locus_tag="OHN27_002420"
     CDS             11334..12074
                     /locus_tag="OHN27_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCQ2286841.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12241..13137
                     /locus_tag="OHN27_002421"
     CDS             12241..13137
                     /locus_tag="OHN27_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2286842.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(13134..14611)
                     /gene="scpC"
                     /locus_tag="OHN27_002422"
                     /pseudo
     CDS             complement(13134..14611)
                     /gene="scpC"
                     /locus_tag="OHN27_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(14635..15420)
                     /gene="scpB"
                     /locus_tag="OHN27_002423"
     CDS             complement(14635..15420)
                     /gene="scpB"
                     /locus_tag="OHN27_002423"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCQ2286843.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(15431..16426)
                     /gene="meaB"
                     /locus_tag="OHN27_002424"
     CDS             complement(15431..16426)
                     /gene="meaB"
                     /locus_tag="OHN27_002424"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCQ2286844.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(16419..18563)
                     /gene="scpA"
                     /locus_tag="OHN27_002425"
     CDS             complement(16419..18563)
                     /gene="scpA"
                     /locus_tag="OHN27_002425"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCQ2286845.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVHQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(18767..19660)
                     /gene="argP"
                     /locus_tag="OHN27_002426"
     CDS             complement(18767..19660)
                     /gene="argP"
                     /locus_tag="OHN27_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCQ2286846.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            19802..20032
                     /locus_tag="OHN27_002427"
     CDS             19802..20032
                     /locus_tag="OHN27_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286847.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLVNGELVRLFAPWSGIP
                     RLLHALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            20088..20747
                     /gene="rpiA"
                     /locus_tag="OHN27_002428"
     CDS             20088..20747
                     /gene="rpiA"
                     /locus_tag="OHN27_002428"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCQ2286848.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21003..22235
                     /gene="serA"
                     /locus_tag="OHN27_002429"
     CDS             21003..22235
                     /gene="serA"
                     /locus_tag="OHN27_002429"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCQ2286849.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            22464..22523
                     /gene="ibsC"
                     /locus_tag="OHN27_002430"
     CDS             22464..22523
                     /gene="ibsC"
                     /locus_tag="OHN27_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCQ2286850.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(22624..23226)
                     /locus_tag="OHN27_002431"
     CDS             complement(22624..23226)
                     /locus_tag="OHN27_002431"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCQ2286851.1"
                     /translation="MTQLPELPLILSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(23247..23430)
                     /gene="ssrS"
                     /locus_tag="OHN27_002432"
     ncRNA           complement(23247..23430)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OHN27_002432"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23472..23801)
                     /gene="zapA"
                     /locus_tag="OHN27_002433"
     CDS             complement(23472..23801)
                     /gene="zapA"
                     /locus_tag="OHN27_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCQ2286852.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            23969..24547
                     /gene="ygfB"
                     /locus_tag="OHN27_002434"
     CDS             23969..24547
                     /gene="ygfB"
                     /locus_tag="OHN27_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCQ2286853.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24573..25898
                     /gene="pepP"
                     /locus_tag="OHN27_002435"
     CDS             24573..25898
                     /gene="pepP"
                     /locus_tag="OHN27_002435"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCQ2286854.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQDEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            25895..27073
                     /gene="ubiH"
                     /locus_tag="OHN27_002436"
                     /gene_synonym="visB"
     CDS             25895..27073
                     /gene="ubiH"
                     /locus_tag="OHN27_002436"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCQ2286855.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            27096..28298
                     /gene="ubiI"
                     /locus_tag="OHN27_002437"
                     /gene_synonym="visC"
     CDS             27096..28298
                     /gene="ubiI"
                     /locus_tag="OHN27_002437"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCQ2286856.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(28372..>28447)
                     /locus_tag="OHN27_002438"
     CDS             complement(28372..>28447)
                     /locus_tag="OHN27_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2286857.1"
                     /translation="NTKSKNNDIRKYNAKTEVKLVYFK"
BASE COUNT         6791 a         6982 c         7643 g         7031 t
ORIGIN      
        1 ctgctcaatt gctttcatta aaccgcacga gtattttatt tttaccgccg gacggtaccc
       61 taaaaataaa ataaggataa tttatgcggc ttagtgatta ttttccagaa tcatcaatct
      121 cagttataca ttcagcaaaa gattggcagg aagctatcga tttctcgatg gtatcattgc
      181 tggataaaaa ctatatcagc gagaattaca ttcaggcaat taaagattcc accattaaca
      241 atggccctta ttatattctc gcaccaggcg tggcaatgcc tcatgcgcga ccggaatgtg
      301 gggcgcttaa aaccgggatg tctttgacat tacttgaaca aggtgtttat tttccgggga
      361 atgacgaacc aattaaatta ctcatcggac tctctgctgc cgatgccgat tcgcacattg
      421 gcgctattca ggcgttaagt gaattactgt gcgaagaaga aatactcgaa caactcttaa
      481 cagcatcatc agaaaaacaa ttagcggaca ttatcagccg cggataatta ctctctcctc
      541 tacaggataa tattatggaa aacaagtctg ctcgtgcaaa ggtccaggct tttgggggct
      601 ttttgactgc aatggtcatc cccaatattg gtgcttttat tgcctggggt tttattactg
      661 cgttatttat tcccaccggt tggctgccta acgaacattt cgccaaaatt gtcggcccga
      721 tgattaccta tttattgccc gtgatgattg gttctacagg tggtcatctg gtcggcggta
      781 aacgcggggc ggtcatgggc ggaataggta ctattggtgt gatcgttggc gcagagatcc
      841 cgatgttcct tggctcaatg atcatggggc cgctcggtgg gttggtcata aaatatgtcg
      901 ataaggcact ggaaaaacgc atacctgccg gttttgagat ggttatcaat aacttctcat
      961 taggtatcgc ggggatgctc ctttgtctgc tgggttttga agttatcggc ccggcggtgt
     1021 taattgccaa tactttcgtc aaagagtgta ttgaggcgct ggtacatgcg ggttatctgc
     1081 cattgttgtc agtcatcaat gaaccggcga aagtgctttt cctcaataat gcgatcgatc
     1141 agggcgtcta ttatccgctg ggaatgcaac aggcttcggt taacggtaaa tccatcttct
     1201 ttatggtggc ctctaaccca ggtccgggcc tggggctgct gctggcgttt accttgtttg
     1261 gtaaagggat gagtaaacgt tctgcgcccg gggcgatgat tattcacttc ctcggtggga
     1321 tccacgaact gtatttcccg tatgtgctga tgaagccgct gaccattatt gccatgattg
     1381 cgggcggtat gtctggcacc tggatgttta acttactgga cggtggtctg gtggctggcc
     1441 caagtccggg gtctatcttt gcttacctgg cactgacgcc gaaaggctcg ttcctggcga
     1501 caattgccgg tgttacggta ggtaccctgg tgtcctttgc tattacttcg ctgatactga
     1561 agatggaaaa aacggtggaa acggagagcg aagatgagtt tgctcagtca gccaatgcgg
     1621 ttaaggcgat gaaacaagag ggtgcattct cgttaagcag ggttaagcgt atcgcctttg
     1681 tttgcgatgc ggggatgggc tccagtgcga tgggcgcgac caccttccgt aaacgcctgg
     1741 aaaaagcggg gctggcaatt gaagtaaaac attacgccat agaaaacgtg cctgcggatg
     1801 cggatatcgt cgttactcat gccagtctgg aagggcgcgt gaaacgtgtg acggataaac
     1861 cactgatatt gattaataac tatattggcg atccaaaact cgacacttta tttaatcaat
     1921 taaccgccga acataaacac tgattacaga ggtaaaaatg aaaaccaaag ttgctgctat
     1981 ttatggcaag cgggatgtcc gtctgcgcgt atttgaactg ccagaaatta ccgataatga
     2041 attactggtg agtgtaattt ctgacagcgt ctgtttatcg acctggaaag cggcgttact
     2101 cggtagtgaa cataaacgcg tacccgacga tttagaaaat catccggtca ttaccgggca
     2161 tgaatgtgcc ggggttattg tcgaagtggg taaaaatctc actggcaaat ataaaaaagg
     2221 ccagcgtttt gtattgcaac cggcgatggg gttaccaagc ggatattcag cgggctacag
     2281 ctacgaatat tttggcggca atgccactta tatgattatt cccgaaatcg ccattaattt
     2341 gggctgcgta ttaccgtatc acggctctta ttttgctgcg gcgtcgctgg cagagcctat
     2401 gtgctgcatt attggtgctt atcatgccaa ttatcacacc acgcaatatg tttatgagca
     2461 tcgcatgggc gtcaaacctg gcggcaatat tgcactgctg gcgtgtgcag gtccgatggg
     2521 cattggcgct atcgattacg ccattaacgg cggcatacaa ccgtcgcggg tggtggtggt
     2581 cgatatcgac gacaaacgtc tggcgcaggt acagaagctg ctgccggtgg aactggcggc
     2641 cagtaaaggc attgagctgg tgtatgtgaa taccaaaggg atgagcgatc ctgtccagat
     2701 gctgcgggcg ctgacaggag atgccgggtt cgatgacatt tttgtttatg cggcggtgcc
     2761 tgctgtcgtt gagatggctg atgaattact ggcggaagat ggctgtctga acttctttgc
     2821 cgggccgacg gataaaaact tcaaagtgcc gtttaatttc tacaacgtcc attacaacag
     2881 cacgcacgtc gtcggtacat ctggcggttc aacggacgac atgaaagagg cgattgccct
     2941 tagcgccact gggcagttac agccgtcgtt tatggtgacc catatcggtg gcctggatgc
     3001 ggtgccagaa accgtgctca atctgccgga tatacctggc ggtaaaaaac tcatttataa
     3061 cggcgtgacc atgccgctca ctgccattgc cgattttgcc gaaaaaggca aaaccgatcc
     3121 gctgtttaaa gagttggcgc ggctggttga ggaaacgcac ggcatctgga atgaacaggc
     3181 cgagaaatat ctgctggcac aatttggcgt tgatatcggg gaggccgcgc aatgatgtcc
     3241 ctggcgtggc cattatttcg cgttacggaa caggcagcgt tggctgcctg gccgcaaacc
     3301 ggatgtggcg acaaaaataa aattgatggc ctggcggtca ccgcgatgcg ccaggcatta
     3361 aacgacgtcg cttttcgtgg acgagtggtt atcggcgaag gagaaattga ccatgcgccg
     3421 atgctgtgga ttggcgaaga ggtgggcaaa ggcgatgggc cagaagtcga tatcgcggtt
     3481 gatcccattg aaggcacgcg gatggtggcg atggggcaga gcaatgcgct ggcggtcatg
     3541 gctttcgccc ctcgagatag cctgttacat gcgcccgata tgtatatgaa aaagctggtt
     3601 gttaatcggc tggcggcggg cgcgattgat ctgtctttgc ctctgacgga caacctgcgc
     3661 aacgtggcga aagcgttagg caaaccgctg gataagctgc gcatggttac gctggataaa
     3721 ccgcgcctga gtgccgcgat tgaggaagca acacagctgg gcgtgaaggt ttttgccctg
     3781 ccggatggcg atgtcgccgc cagcgtgctg acctgctggc aggataatcc ttacgatgtg
     3841 atgtacacca tcggcggtgc gccagaaggc gtgatttctg cctgtgccgt taaagcatta
     3901 ggcggggata tgcaggccga gctgattgac ttttgccagg ctaaagggga ttacacggaa
     3961 aatcggcaga ttgcagagca ggagcgcaag cgttgcaagg caatgggcgt tgacgttaac
     4021 cgtgtgtact cgctcgatga actggtgagg gggaacgata tcctctttag cgctacgggc
     4081 gtgacgggcg gtgagctggt gaacggtatc caacagacgg cgaatggggt gcggacgcag
     4141 acattactga tcggcggcgc ggaccaaacg tgtaatataa tagactccct gcattgatgg
     4201 tgatctatcg gaagaccaat ggcgacgctg acagaagatg atgtgcttga gcaactggat
     4261 gcacaggaca atttattttc atttatgaaa actgcgcata ccattttgct ccaggggata
     4321 cgccagtttc tgccgtcgct gtttgtcgat aacgatgaag agatcgtgga atatgcagtg
     4381 aagccgttac tcgcccaaag cggcccgctt gacgatattg atgttgcact gcgtttgatt
     4441 tatgcgttgg ggaaaatgga taaatggttg tacgccgata tcacgcattt ttcccagttc
     4501 tggcattacc tgaacgaaca ggatgaaacg cccggatttg ccgatgacat gacctgggat
     4561 tttatcagca acgtcaatag cataacctgc aatgcgacgc tctatgacgc gttgaaagcg
     4621 atgaagttcg ccgatttcgc cgtctggtca gaggcgcgtt ttagcggaat ggtcaaaacg
     4681 gcgctgacgc tggcagtaac gaccacctta aaggaattaa cgccgtgaaa attgaattaa
     4741 cggtgaatgg gctgaagata caggcacagt accagaacga agagattgaa aatgtacata
     4801 aaccgttgct gcacatgttg gcggccttac agacggtaaa tccgcagcgg cgtacggtgg
     4861 tttttctttg cgctccgccg gggacgggta aatcaaccct aaccaccttc tgggaatatc
     4921 tcgcgcaaca agacccggaa ctgcccgcta ttcagacgct cccgatggat ggttttcacc
     4981 attacaatag ctggctggat gcgcatcaat tgcgcccctt caaaggcgca ccagagacat
     5041 ttgacgttgc gaaactgacg gaaaatctgc gccaggtcgt ggaaggggat tgtacgtggc
     5101 cgcagtacga tcgacaaaag catgatcctg ttgaagatgc gttgcacgtt accgcaccac
     5161 tcgtcatcgt cgaaggaaac tggttgttac tggatgatga aaagtggctg gagttggcat
     5221 ctttctgcga tttttctatt tttattcatg cacctgctca aattctgcgg gagcgcttga
     5281 ttagtcgtaa aattgcggga ggattaacgc ggcaagtagc cgaagcattt tatgcccgca
     5341 ccgatggccc caatgtcgag cgtgtgctga tgaatagccg tcaggccaat ctgatagtag
     5401 agatgactga agaaggccgg tatcacttca caagctgaca atttattcca gttattgtag
     5461 ggttattatt acgccagcct ggaataaagc ttcccacaaa aggggataaa agtgtgatgt
     5521 gagtcagata aatgtcttct tcggctggac aaacattcct tttattccac gtttcgctta
     5581 tcctagctga agcgtttcag tcgattaaat gttcgacaat taaccaatca gtcgcagttt
     5641 gcgacaggta aggtttcccc ggacgatttg ctggattact ctgtctggca aatttgctca
     5701 gggaaaacct tgcaggagat ctatgaccgt acgcgtagcg ataaatggct tcggtcgcat
     5761 cgggcgtaat gtggttcgtg ctttgtatga atccggacgc cgggcggaaa ttaccgtggt
     5821 ggcaatcaac gaactggcgg atgctgcggg catggcgcat ttgttgaaat atgacaccag
     5881 ccatggccgt tttgcatggg aagtacgaca ggaacgcgat caactttttg ttggtgatga
     5941 cgccatccgc gtattgcatg aacgttcact gcaatcgctc ccctggcgtg aacttggcgt
     6001 tgatgtagtc ctcgactgca ccggcgtata tggctcccgc gagcatggcg aagcgcatat
     6061 tgccgccggg gccaaaaaag tgctcttttc acatcctggc agtaacgatc tcgacgcgac
     6121 cgttgtttac ggcgtcaatc aggatcaact tcgtgcggaa caccgcatcg tttctaacgc
     6181 ttcctgtacc acgaattgca taattcccgt catcaaattg ttagatgatg cgtacggtat
     6241 tgagtccggc actgtgacca caattcactc cgccatgcac gatcaacagg ttattgatgc
     6301 ataccatcct gacctgcgtc gcacccgggc agccagccag tcgatcattc cggtcgatac
     6361 taaactggcc gccggtatca cacgattttt tccgcaattt aacgatcgct ttgaagcgat
     6421 tgcggtacgt gtgccaacca taaatgtgac ggcaatcgat ttaagcgtga cggtgaagaa
     6481 acctgtaaaa gccaatgaag tcaacctgtt gctgcaaaaa gcagcacaag gtgcatttca
     6541 tggtatagtt gactatacgg aattgccgtt ggtctctgta gattttaacc acgatccgca
     6601 cagtgccatt gtcgatggca cccaaacccg ggtcagtggc gcacacctga tcaaaacgtt
     6661 ggtctggtgc gataacgaat ggggctttgc taaccgaatg ctcgacacga cgttagctat
     6721 ggctactgtt gctttcaggt aagacgcaag cagcgtctgc aaaactttta gaatcaacga
     6781 gaggattcac catgtctgta attaagatga ccgatctgga tcttgctggg aaacgtgtat
     6841 ttatccgtgc ggatctgaac gtaccagtaa aagacgggaa agtaaccagc gacgcgcgta
     6901 tccgtgcttc tctgccgacc atcgaactgg ccctgaaaca aggcgcaaaa gtgatggtaa
     6961 cttcccacct gggtcgtcct accgaaggcg agtacaacga agaattctct ctgctgccgg
     7021 ttgttaacta cctgaaagac aaactgtcta acccggttcg tctggttaaa gattacctcg
     7081 acggcgttga cgttgctgaa ggtgaactgg ttgttctgga aaacgttcgc ttcaacaaag
     7141 gcgagaagaa agacgacgaa accctgtcca aaaaatacgc tgcactgtgt gacgtgttcg
     7201 taatggacgc attcggtact gctcaccgcg cgcaggcttc tactcacggt atcggtaaat
     7261 tcgctgacgt tgcgtgcgca ggcccgctgc tggcagctga actggacgcg ctgggtaaag
     7321 cactgaaaga acctgctcgc ccgatggtgg ctatcgttgg tggttctaaa gtatctacca
     7381 aactgaccgt tctggactcc ctgtctaaaa tcgctgacca gctgattgtt ggtggtggta
     7441 tcgctaacac ctttatcgcg gcacaaggcc acgatgtggg taaatccctg tacgaagctg
     7501 acctggttga cgaagctaaa cgtctgctga ccacctgcaa catcccggtt ccgtctgatg
     7561 ttcgcgtagc aaccgagttc tctgaaactg caccggctac cctgaaatct gttaacgatg
     7621 tgaaagctga cgagcagatc ctggatatcg gtgatgcttc cgctcaggaa ctggctgaaa
     7681 tcctgaagaa tgcgaaaacc attctgtgga acggtccggt tggcgtgttc gaattcccga
     7741 acttccgcaa aggtactgaa atcgtggcta acgctatcgc agacagcgaa gcgttctcca
     7801 tcgctggcgg cggcgacact ctggcagcaa tcgacctgtt cggcattgct gacaaaatct
     7861 cctacatctc cactggcggc ggcgcattcc tcgaattcgt ggaaggtaaa gtactgcctg
     7921 cagtagcgat gctcgaagag cgcgctaaga agtaaaaaat cacagggcag ggaaacctgc
     7981 ccttgtttca gcgcgctttt agagcacgca atttttcata ctctaaataa ttcgagttgc
     8041 aggaaggcga caagagagtg aatccccagg agcttacata agtaagtgac tggggtgagc
     8101 gaacgcagac gcagcacatg caacttgaag tatgacgagt ataaggcccg acgatacagg
     8161 acaagagaca tgtctaagat ttttgatttc gtaaaacctg gcgtaatcac tggtgatgac
     8221 gtacagaaag ttttccaggt agcaaaagaa aacaacttcg cactgccagc agtaaactgc
     8281 gtcggtactg actccatcaa cgccgtactg gaaaccgctg ctaaagttaa agcgccggtt
     8341 atcgttcagt tctccaacgg tggtgcttcc tttatcgctg gtaaaggcgt gaaatctgac
     8401 gttccgcagg gtgctgctat cctgggcgcg atctctggtg cgcatcacgt tcaccagatg
     8461 gctgaacatt atggtgttcc ggttatcctg cacactgacc actgcgcgaa gaaactgctg
     8521 ccgtggatcg acggtctgtt ggacgcgggt gaaaaacact tcgcagctac cggtaagccg
     8581 ctgttctctt ctcacatgat cgacctgtct gaagaatctc tgcaagagaa catcgaaatc
     8641 tgctctaaat acctggagcg catgtccaaa atcggcatga ctctggaaat cgaactgggt
     8701 tgcaccggtg gtgaagaaga cggcgtggac aacagccaca tggacgcttc tgcactgtac
     8761 acccagccgg aagacgttga ttacgcatac accgaactga gcaaaatcag cccgcgtttc
     8821 accatcgcag cgtccttcgg taacgtacac ggtgtttaca agccgggtaa cgtggttctg
     8881 actccgacca tcctgcgtga ttctcaggaa tatgtttcca agaaacacaa cctgccgcac
     8941 aacagcctga acttcgtatt ccacggtggt tccggttcta ctgctcagga aatcaaagac
     9001 tccgtaagct acggcgtagt aaaaatgaac atcgataccg atacccaatg ggcaacctgg
     9061 gaaggcgttc tgaactacta caaagcgaac gaagcttatc tgcagggtca gctgggtaac
     9121 ccgaaaggcg aagatcagcc gaacaagaaa tactacgatc cgcgcgtatg gctgcgtgcc
     9181 ggtcagactt cgatgatcgc tcgtctggag aaagcattcc aggaactgaa cgcgatcgac
     9241 gttctgtaag atattccttt ctgcttatct caaggcccgc tctgcgggtc tttttttcgc
     9301 caaaagcaaa attgcctgat gcgctacgct tatcaggcct acacgtatcc tgcaatatat
     9361 tgaatttgca ggtttttgta ggccggataa ggcgttcacg ccgcatccgg catgacaaag
     9421 cgcacattga tatctgtctg agccccgctc tgcggggatt ttttcgccaa aagcaaggtg
     9481 attcagatga gaaatctgtg atctatttgg caaaattatg ctttattgtt tacccttgtc
     9541 agactgcccg tcataaggcg gcggagtgta tttctccatt ttgagtcagt tgaaaaggaa
     9601 tattgaatgg aagatttgaa tgttgtcgat agcataaacg gcgcgggaag ctggctggta
     9661 gctaaccagg cgctgctgct aagttatgca gtaaacatcg tggcggcact cgcgatcatc
     9721 atcgttggtt tgattatcgc gcggatgatt tccaacgcgg tgaatcgcct gatgatctcc
     9781 cgtaaaatcg atgccactgt tgctgatttt ctttctgcat tagtccgtta cggtattatc
     9841 gcctttacgc taatcgctgc actgggacgc gtgggtgtac aaaccgcgtc agtcattgct
     9901 gtactcggtg ccgcaggctt agctgttggt ctggctttgc aggggtcact gtctaacctg
     9961 gccgctggcg tgttacttgt catgttccgc ccgttccgtg ccggagaata tgttgacctg
    10021 ggcggcgtag ccggtactgt gctgagtgtg cagattttct ccaccaccat gcgtactgca
    10081 gacggtaaaa ttatcgttat tccgaacggt aaaattattg ccggaaatat tattaacttc
    10141 tcccgcgagc cagttcgccg taacgaattt attattggcg tggcgtatga ttccgatatc
    10201 gatcaggtta agcagatcct gaccaatatt atccagtctg aagatcgcat tttgaaagat
    10261 cgcgaaatga ctgtgcgcct gaacgaactt ggtgcatcgt cgattaattt cgtggtccgc
    10321 gtctggagca acagcggcga tctgcaaaac gtgtactggg atgtgctgga gcgtattaaa
    10381 cgtgaatttg atgccgccgg tatcagcttc ccgtacccgc aaatggatgt gaactttaag
    10441 cgggtgaaag aagacaaagc tgcgtaatca acgctgaggc cagataatac tctctggcct
    10501 ctctcttatt agtttttctg attgccaatt aatattatca atttccgcta ataacaatcc
    10561 cgcgatatag tctctgcatc agatacttaa ttcggaatat ccaacgtgtt ttcttattac
    10621 tttcaaggtc ttgcacttgg ggcggctatg atcctaccgc tcggtccaca aaatgctttt
    10681 gtgatgaatc agggcatacg tcgtcagtac cacattatga ttgccttact ttgtgctatc
    10741 agcgatttgg tcctgatttg cgccgggatt tttggtggca gcgcgttatt gatgcagtcg
    10801 ccgtggttgc tggcgctggt cacctggggc ggcgtagcct tcttgctgtg gtatggtttt
    10861 ggcgctttta aaacagcaat gagcagtaat attgagttag ccagcgccga agtcatgaag
    10921 caaggcagat ggaaaattat cgccaccatg ttggcagtga cctggctgaa tccgcatgtt
    10981 tacctggata cttttgttgt actgggcagc cttggcgggc aacttgatgt ggaaccaaaa
    11041 cgctggtttg cactcgggac aattagcgcc tctttcctgt ggttctttgg tctggctctt
    11101 ctcgcagcct ggctggcacc gcgtctgcgc acggcaaaag cacagcgcat tatcaatctg
    11161 gttgtgggat gtgttatgtg gtttattgcc ttgcagctgg cgagagacgg tattgctcat
    11221 gcacaagcct tgttcagtta ggcgctatct gatggaaaaa taaaacagag gcgctaagct
    11281 tgcctccaga ggtcctgaat tttgggcaag tcaattaaca cggagagact aacgtgaagt
    11341 tcaaagttat cgccctggcg gcattaatgg gtattagcgg gatggcagcg caggctaacg
    11401 aattgccgga tggaccgcat attgtcacct ccggtacggc aagcgtggat gcggtgccag
    11461 acattgccac tcttgcgatt gaagttaacg tggccgcgaa ggatgccgct actgccaaga
    11521 aacaggcaga tgagcgcgtc gcacaataca tttccttcct tgaactcaat cagatcgcga
    11581 aaaaagatat cagctcagcg aacttacgca cccagccaga ttatgattat caggatggta
    11641 aaagtatcct taaaggctac cgcgctgtga gaacggtgga agtcacgctc cgtcagttag
    11701 acaaactgaa ttccttgctg gatggcgcgc tgaaggcggg tcttaacgaa attcgttctg
    11761 tgtcgctggg cgtggcgcag ccggatgcct ataaagacaa agcgcgtaag gcagcgattg
    11821 ataacgcgat tcatcaggcg caggaactgg cgaacggctt tcatcgtaaa ctggggccgg
    11881 tatatagcgt gcgctaccat gtttccaact atcagcccag cccaatggtg cggatgatga
    11941 aagccgatgc cgcgccggtg tccgcccagg aaacttacga gcaggccgct attcagtttg
    12001 atgatcaggt cgatgtggtc ttccagttag aacctgtgga tcaacaaccc gctaaaacac
    12061 ctgcagcaca ataatatttg tagcccgacg tattcgtcgg gctaatgcct ttacccgata
    12121 ttgcctcctt tttctgtaat tttctgtaaa accatgacgt aatcacctat ctttgctcgc
    12181 ggtttcatca aggataggct gactgaaacg gttaagcgga caaggatgcg ggggaggggg
    12241 atggacattt ttatttcgaa aaagatgcgc aatttcattt tattagcgca aaccaataat
    12301 attgcccggg cggcagaaaa aatccatatg acggcttcgc catttggtaa aagcattgcc
    12361 gcgctggaag aacaaattgg ctatacgcta tttacccgca aagataataa tatcagcctc
    12421 aataaagccg gacaggaact gtatcaaaaa ctgttcccgg tttatcaacg gctttctgcg
    12481 attgataatg aaatccataa ctcagggcgt cgttcacgag aaattgtgat tggcatagat
    12541 aatacttatc cgacaattat tttcgatcag ttgattagtc tcggagataa gtatgaaggc
    12601 gtgaccgccc agccagttga gttcagcgaa aatggcgtta tcgataatct ttttgaccgc
    12661 cagttagatt ttattatctc cccacagcat gtatctgcac gagttcagga actggaaaac
    12721 ctgactatca gtgaactacc accgctgcga ttaggctttc tcgtttctcg ccgttatgaa
    12781 gagcgacaag agcaagagct gcttcaggag ctgccctggc tacaaatgcg ctttcagaac
    12841 cgggccaact tcgaagcaat gattgatgcg aatatgcgac cttgtgggat caatccaacc
    12901 attatttatc gtccctacag ttttatggcc aaaatcagcg ccgtggagcg cggacatttt
    12961 ctgacggtga tcccgcattt tgcctggcga ctggtgaatc cggcaacgct gaaatatttt
    13021 gatgcaccgc acaggccgat gtatatgcag gaatatcttt attcaatcag aaatcatcgt
    13081 tataccgcca cgatgcttca gcatattgct gaagatcgtg acgggacgag tcattaaccc
    13141 agcatcgagc cggttgcaat taaattacgg tgtaagtcga agacgtggct aagatcgtgg
    13201 tgaatatgtc cgccaggcgc attttccaga tagcgatgca gataatcccg atacatagga
    13261 tgtgcacaat tatcaatgat agtgcgggcg cgttgaagcg gggaaagacc gcgcagatcg
    13321 gcgatccctt gttcggtgat gatcactttg acgctgtgtt cgctgtgatc aacatggctg
    13381 cacattggca cgacggttga gatcttgcct tctttagcaa tcgacggggc cataaagatc
    13441 gacagatacg cgttgcgttc aaaatcaccg ctgccgccga tgccgttcat cagatcgacc
    13501 cggctacgtg tgttgagttg gcatgcccgt aaatatcaaa ctccaggccg acgttcagag
    13561 cgatgacgcc cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca
    13621 caatgcggct ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga
    13681 ttgtcaggct ggaggcgctg gccccgctga ttttgccggt ttccagtaaa tgcaccaccg
    13741 attcctgtag cacttccgaa tacatcataa acggaggaat taccgggttt tcccccagac
    13801 gcgccattac cgcattattg atattgccca cgccactttg cagcggcaga aattccggcg
    13861 gaatacgccc atgcgccatt tcctgcaata agaacgtgac cacgttatcg gcaatctgct
    13921 ggcacatggg attttgctta tccagcatat taccggcgtc gggcaagttg gtttccacga
    13981 cggcgacaat ctttttcgga tcgatttgca catagcgggt accgacgcga tccattgcat
    14041 ggaagatcga cacgctattg cgccgcggtg gcgcgccagg aatcacaata tccgccagtt
    14101 ctgcaacgcg cggatcgtga tagtgattga gttcaatgat cactttcttc gcccgcagca
    14161 gccaggtcgg cgcattaccg atcccgctgg ttaaccagac tcgaccatcc ggtgccagtg
    14221 ccgatgcttc aatgacggca acatcaatgt cgccgaagaa accgtaattg accatttgcg
    14281 ccacttcgct caaatgcagg tcaacgaaac tcaccgcgcc ctgattgatc tttttacgta
    14341 aaccggacga tgtttgatat ggcgcacgcc aggaaacagc atcggcgtca gaaagtacat
    14401 cgtcagcggc ggcgctgatt gacgcacccg tcagaaggcg aatttgatac ggctttttgg
    14461 cctcatgctg ttcgttagct ctgcgggcaa tcgcggtggg tagggctttc ggcgaacccg
    14521 ccggggtaaa gccgctaaat gccaccatgt cgttatgctg gataatttct gccgcttcat
    14581 tggcggtcat ccttgtccac tgagtttcca tttttactcc ttcgttcgca gggattaatg
    14641 accaacgaaa ttaggtttac gtttttcgag gaaagcgttc atcccttcct ggtaatcttc
    14701 gctgtcatac accgcgcggc gcatcccctg aatacgttca aattcatcgg agttcatggt
    14761 gtgtgcttcg cccagtacac gcagctcttc tttgataacg gcaatggcta acggcgcttt
    14821 ctcagagatg tggtgcgcca tttgtaaggt gaaatcttcc agttcttcca cttccacaac
    14881 atggttgagg atgccgacag ccagcgcgcg ctgggcggtg attggcgaag cggtaaaaat
    14941 cagctctttg acaatgtgga agcccgcgtc gcgggtcagg ttgtgaatgc cgaccaggtt
    15001 atacgggacg ccgaggttta caggcgtcat tgagaaggtt gaggtactgg cggcgatgat
    15061 cagatcggaa ctcatgatca tttcaaatgc gccaccccaa acactacctt ccaccatcga
    15121 aatgatcggt ttcgggaatt tttggatcat gcgggtgatt tgacgcaatg gatcatcata
    15181 ggagagcgga tcgcgaccgc cagacggcag ttcgtgaata tcgtgacctg cggagaagac
    15241 tttggatcca ctcggtgcgc gcaaaatgat acagcgaatt tccggccggt tgagatcgct
    15301 taacgcctgc ataagatcat caataaagac tttacttaag gcattaagtt ttcggccata
    15361 gttaaactca atgaccgcca ctttgttgat agtgacaacg ttaacatact gataagacat
    15421 aaaaattcct ttaatcaaaa tattgcgtct ggataaattc actgagctgc cgcaggccgg
    15481 tgcgcggtga gagcgtattg tttttgaccg ctaaaagcgt ctggcggtaa tagcgatcga
    15541 aatcttcatt cgcgaacagg tgattcagta cttcttcttc ggtctgctta cgcagccatt
    15601 ccaccgattg ttgttgccgc acttgttgta aacgaccact ggcagttagc gcggttttga
    15661 agtcgatgat ggcgtgccag atctcatcga ttccacgttt ttccagtgcg ctacaagtca
    15721 gaacccgtgg ctgccattcg tcgtatttac gtcgcagaat atgcagggca ctctcgtaca
    15781 tatgccgggc aatggcgaca ttggtatggt tatcgccatc gtctttgttg ataacgatca
    15841 gatcagccac ttccatcagc ccttttttaa tgccctgcag atcatcgccg ccaccggcaa
    15901 tttgcaacga gataaaacag tccaccatgc gggcgacttc tgtttccgac tgcccgacgc
    15961 caaccgtttc gacaatcact acgtcataac ccgctgcttc gcataacagc attaattccc
    16021 gcgctcgctg actggcaccg cccagatgac cggaggatgg taccgggcga ataaacgccg
    16081 cttcggcacg cgccaggtca ttcatgcggg ttttatcccc gagaatgcta ccgccagtga
    16141 ccgggctgct gggatcgacc gcaataaccg cgacctttaa tccctctcga atcaacaaca
    16201 tgccaaaggc ctcaagaaag gtacttttcc ccgcgccggg ggtgccggta acgcccagtc
    16261 gcagggtgtt accgcagtac ggcataatgg catcaagcag ctgcgtactt agtgcctgat
    16321 gacgcgggtg acggctttcc accagcgtca tggcctgggc gagtgtggca cgctcaccct
    16381 gacgtaagcg gcgaatactt tctgccagcg tggcttcatt aatcatgatg ctggcttatc
    16441 agattcagta cgtcgcgcac actgtcgagc ataggtgtac ctggaccata aatcgccgcc
    16501 acgccgcgct cttgcaggaa ggcgtaatcc tgcggcggaa tgacgccacc cgcgaccacg
    16561 cagatatctt cgcgtcccca ttttttcagc gcttcgacca gttccgggat cagcgtttta
    16621 tgaccggcag ccagtgagga tgcgcccact acgtgaacgt cgttttctac ggccaggcgg
    16681 gcgatctctt caggtgtaga gaacatcggg cttaaatcta cgtcgaaacc gagatcggaa
    16741 taggcgctgg cgatcacttt cgcgccgcga tcgtgtccat cctggcccat cttagcgatc
    16801 agaatgcgcg ggcgacgacc attgtcggca aggaactgct ccgtttgcgc aacaatggca
    16861 tcgaactcgg aggccgattt ctcagactga tgatagcttt gcgcaatcac gccggtaaca
    16921 cactggcttg gcaccagata acggtcgaaa gcgacttcca gcgcatcgga aatttcaccc
    16981 agggtggcgc gaacgcgagc ggcattaaca gcggcagcca gcaggttttc gttatgctgt
    17041 gcggcgtgag tcagggcgtt caacgcggcg gttacggcgg catcatcacg ggtggcgcga
    17101 atgcgttcca gcgaagcaat ttgctcgtta cgcaccatca cgttgtcgat ctcaagtaca
    17161 tcggtttcgt cttcgtgatc cagtttgtac ttgttgacac caacgatgac acgcttgccc
    17221 tggtcgatca gcgactgttc gcgcgctgag gcctcttcga tcattcgttt tggcagacct
    17281 gcttcgatcg ctttcgccat gccaccggct tcgtcgatct gttggataat agctctggct
    17341 tgtttgacga tttgatcggt cagcgactca atgtaatagg atccggccag tggatcgacg
    17401 gtgcggcaga gttctgattc ttcctggatg atgatctggg tgttgcgggc aatgcgtgct
    17461 gagaaatcgg taggcaaacc aagcgcttcg tcaaaggcgt tggtatgcag tgactgagta
    17521 ccgcccagcg tcgcagccag cgcttcaatg gtggtgcgga taacgttgtt atacggatcc
    17581 tgttcagtca ggctccagcc tgaggtctgg cagtgggtac gcagcgccag tgatttcggg
    17641 tcctgtgcgc caaatccact gaccgcttcg ctccataaat aacgtgccgc acgcaacatg
    17701 gcgacgttca taaacagatc catgccgatg ccgaagaaga acgacaggcg aggagcgaag
    17761 tcatcaattt tcagtccggc agagattgct gctttgatgt actcaatccc atcagcgagc
    17821 gtaaatgcta cctgctgcac gcagttggca cccgcttcac ccatgtggta accgctgata
    17881 ctgatggtat taaatcgcgg catgttgccg gaacaccagg cgatgatgtc ggcgataatg
    17941 cgcattgacg gttttggtgg gtaaatatag gtgttgcggc agaggtactc tttgagaata
    18001 tcgttttgaa tggtgccggt cagtttatca ggtgtaacac cttgctcttc tgcggcgacg
    18061 atataaaacg ccagtactgg tagcactgcg ccattcatgg tcatcgaaac cgacatttta
    18121 tccagcggga tctggtcgaa caggactttc atatcttcca cggtgtcgat agcgacgccc
    18181 gctttgccga cgtcgcccgc cacgcgcggg ttatcggagt cgtagccacg gtgggtggca
    18241 aggtcaaacg caacggaaag acctttttgc ccggcggcca ggttacggcg ataaaaagcg
    18301 ttggactctt ttgctgttga aaaaccagca tactgacgga tggtccacgg ttgggcggta
    18361 tacatagtgg cacgcgggcc acgaacgtag ggcggcaaac caggaagggt acctgtcacc
    18421 tccagattat cgagatcggc ttcggtatac agcggcttga tggcgatccc ttccgcggtt
    18481 tgatgaacca gcgagtcgac agttttctcc cgacggctca attccttgtt ggcaagctgt
    18541 tgccactcct gcacgttaga cattatttgc tccgtaaaac gcaatcaaaa tgagggtatt
    18601 tgcgcctacg gtgagctttt tacgtgggtt tgtcgccagc aaaaaaaatg cattcagacg
    18661 tcgttttttg ccgcctgaga tttgtctatt ttgtgatgtg attcaaggaa tacaaaaaga
    18721 agtgaggaca acgcctgata tgtgctatca ggcattattt gatggattaa tcctgacgaa
    18781 ggactttgtg accataatcg agtaacgcat cagtgacttt acgcatcatg cggctttcag
    18841 gagcaaagcg gtgccagtag agcatccgtc gttgaaatag cccaggcgtt aagtcaatca
    18901 gttcaccgct ggccagctct ttctcgattt gcaggtgcgg gatcatacag caggtggtgc
    18961 cctggcgagc aagttgtacg aacgcttctg aagaattaac gatatggcag ggcacgctgc
    19021 ctggaggcag atcgaagttt tgctgcaaaa aggcctggtg catatcgtca agatggtcaa
    19081 acgcgaccac tggcgctttc agtaatgccg aacgcgttac gccgttaggg aaatattttt
    19141 cggcaaaggg ttttgagctg acgaacagat agtcgagcgc accaagttta tcgacaagac
    19201 aactcggcag cgcctgatgt tgaatactca ccgcgccgac cacttcgccg cggcgcagac
    19261 gttcctgagt gcgggtttca tcttctacct gcaagttgag gcggataggc gaatcagcca
    19321 acacaggagc cagtgcagga agcaaccacg tcgccagact gtcggcgttg accgccagtg
    19381 aaagcagcag cggagtcgaa ccggtttgtt catcgcccag ccactcttct tccagcaact
    19441 ccacctggcg cagcagtgcc agcagttttt gcccttgttc cgtcgggcgc ggcggtacgg
    19501 tacgcaccaa cagcggctgc ccgaacatat tttccagttg cttaatgcgc tgtgagacgg
    19561 ctgattgtgt aatgcacagc ttttgtgccg cgcgctcaaa tcctcgttca cgtatcaccg
    19621 catccagtgc ctgtaatgtt ctgtagtccg ggcgtttcat tgctgttgtc tgctcctggt
    19681 aaatgctttt tcctgcacta tgacacaatt ttgtgtcagg ttgcatatac cgaagccgtc
    19741 ggcgggtaac atttgtattc gtggttaaac gcgcagctta aaagatcagg gatttgcgct
    19801 aatgcatttc gctcagcgag ttcgcgccct ggttgtgttg aatggcgtgg cgttattgcc
    19861 tcaatttgcc tgtaaacagg ggcttgtgaa cggtgaactg gtgcgcctgt ttgctccgtg
    19921 gagcggcata cccagattgt tgcatgcctt atttgcgggg cgaaagggga tgcctgccat
    19981 tgcgcgatat tttatggatg agttaaccac gcggcttgcc aacggggtct gaatcgcttt
    20041 ttttgtatat aatgcgtgtg aaatttcata ccacaggcga aacgatcatg acgcaggatg
    20101 aattgaaaaa agcagtagga tgggcggcac ttcagtatgt tcagcccggc accattgttg
    20161 gtgtaggtac aggttccacc gccgcacact ttattgacgc gctcggtaca atgaaaggcc
    20221 agattgaagg ggccgtttcc agttcagatg cttccactga aaaactgaaa agcctcggca
    20281 ttcacgtttt tgatctcaac gaagtcgaca gccttggcat ctacgttgat ggcgcagatg
    20341 aaatcaacgg ccacatgcaa atgatcaaag gcggcggcgc ggcgctgacc cgtgaaaaaa
    20401 tcattgcttc ggttgcagaa aaatttatct gtattgcaga cgcttccaag caggttgata
    20461 ttctgggtaa attcccgctg ccagtagaag ttatcccgat ggcacgtagt gcagtggcgc
    20521 gccagctggt gaaactgggc ggtcgtccgg aataccgtca gggcgtggtg accgataatg
    20581 gcaacgtgat cctcgacgtc cacggcatgg aaatccttga cccgatagcg atggaaaacg
    20641 ccataaatgc gattcctggc gtggtgactg ttggcttgtt tgctaaccgt ggcgcggacg
    20701 ttgcgctgat tggcacacct gacggtgtca aaaccattgt gaaatgatct gacgggggaa
    20761 cctcccccgt taaaaaaatt ctcttcatta aatttggtga catgtgtcac gtttttacct
    20821 ggctattgtc gattgctcta aataaatctt ctaaaccagc atattcatcc aagaattacc
    20881 tttgcgtgat atttcctcaa catcgcgacg caaacgttca tattgccgca atattatttt
    20941 ttgatatgtt gaaaggcgga cgcaaatccg cacacaacat ttcaaaagac aggattgggt
    21001 aaatggcaaa ggtatcgctg gagaaagaca agattaagtt tctgctggta gaaggcgtgc
    21061 accaaaaggc gctggaaagc cttcgtgcag ctggttacac caacatcgaa tttcacaaag
    21121 gcgcgctgga tgatgaacaa ttaaaagaat ccatccgcga tgcccacttc atcggcctgc
    21181 gatcccgtac ccatctgact gaagacgtga tcaacgccgc agaaaaactg gtcgctattg
    21241 gctgtttctg tatcggaaca aaccaggttg atctggatgc ggcggcaaag cgcgggatcc
    21301 cggtatttaa cgcaccgttc tcaaatacgc gctctgttgc ggagctggtg attggcgaac
    21361 tgctgctgct attgcgcggc gtgccggaag ccaatgctaa agcgcaccgt ggcgtgtgga
    21421 acaaactggc ggcgggttct tttgaagcgc gcggcaaaaa gctgggtatc atcggctacg
    21481 gtcatattgg tacgcaattg ggcattctgg ctgaatcgct gggaatgtat gtttactttt
    21541 atgatattga aaataaactg ccgctgggca acgccactca ggtacagcat ctttctgacc
    21601 tgctgaatat gagcgatgtg gtgagtctgc atgtaccaga gaatccgtcc accaaaaata
    21661 tgatgggcgc gaaagaaatt tcactaatga agcccggctc gctgctgatt aatgcttcgc
    21721 gcggtactgt ggtggatatt ccggcgctgt gtgatgcgct ggcgagcaaa catctggcgg
    21781 gggcggcaat cgacgtattc ccgacggaac cggcgaccaa tagcgatcca tttacctctc
    21841 cgctgtgtga attcgacaac gtccttctga cgccacacat tggcggttcg actcaggaag
    21901 cgcaggagaa tatcggcctg gaagttgcgg gtaaattgat caagtattct gacaatggct
    21961 caacgctctc tgcggtgaac ttcccggaag tctcgctgcc actgcacggt gggcgtcgtc
    22021 tgatgcacat ccacgaaaac cgtccgggcg tgctaactgc gctcaacaaa atttttgccg
    22081 agcagggcgt caacatcgcc gcgcaatatc tgcaaacttc cgcccagatg ggttatgtag
    22141 ttattgatat tgaagccgac gaagacgttg ccgaaaaagc gctgcaggca atgaaagcta
    22201 ttccgggtac cattcgcgcc cgtctgctgt actaattccc cttctctgaa aatcaacggg
    22261 caggtcactg acttgcccgt ttttttatcc cttctccaca ccgttgccgc gttgcgcatt
    22321 ctactttgcg agtctgctcc agaaaataaa tcaaagtcag atcaatgcgt tgtgtttatt
    22381 taatggggat ttacgatggc agggcagcat ggggctgtaa cggtaaagcc ctcaccgaag
    22441 cgagggcttg aaggagaagg gttatgatgc gacttgtcat catactgatt gtactgttac
    22501 tcataagttt cagcgcttat taacagtcag tctcagggga ggagcaatcc tcccttaccc
    22561 ttactcacta aattaggtca aagaatcaac gatgtcaatc agggcgatgc aggtgtgtcg
    22621 cccttaccac tcccagactt tcgacggtgt aaccaccgca ggcagtggga tatcccactc
    22681 ttcaacgggg agtttttcca ccaactgaca atcatgcgca taacccaccg gttgcatttt
    22741 atagtgctgc cagttttgta aggtccgatc ataaaaacct ccgcccattc ccaggcgctg
    22801 accgtactca tcaaaggcga ccaggggggt gatcaggacg tctaatcggg aaagggggag
    22861 cacgtcacgc acatccaatt ttggctcatg gatcttcaac ctgttcatca ccagttcgct
    22921 ttgcggatgg taattcagga acagcaaatt accggcacta aagggatgca aaactggaag
    22981 atatacgcgc ttaccggcgc gccagagttg ttctatgagt ggctgggtgt cgagttcgcc
    23041 atcaaaagag aggaatacag cgaccgtatg tgccatcacc accgggggat aagtcatcat
    23101 ccgggtagcg gcttgttgac ccatttcctg ctgttgttcc ggcgttaacg cacgacgacg
    23161 ttgccgaatc attttgcgga tttcttgtcg ggataatatc agtgggagtt ctggtagttg
    23221 cgtcatggct ggtgccagat aagaagggaa tctccgagat gccgccgcag gctgtaaccc
    23281 ttgaaccctt ggttcaaggt gaatgtgtcg tcgcagtttt aaggcttctc ggacggaccg
    23341 agcatgctca ccaaccgcgg agcgccacat tcttgtggta tgaaatatcg gctcagggga
    23401 ctggcccgct tgcgaacatc tcagagaaat tttgtcttca cggttactct accacagtaa
    23461 accgaaaagt gtcattcaaa gttttggtta gttttttcgg tgatgcgacc ttgttcaagt
    23521 aacgcttgtt ctatggtctg ctgcagcatc cgaatacgct gttccatact tgccgcgtag
    23581 tcacgagtct tcgctttttc ttgcgctaac tcatagctga tattcaatgc ggcaatgaag
    23641 accaactgtt cagtatttgt gactctagtg cgttctttca gatcttgcaa ccgttggttc
    23701 agatcgtccg ctgcctgatt caacgcatcc ctttggtcag gcgggcagtt cacacgcagt
    23761 gaacggccaa aaatttggat atcgacgggt tgtgcagaca tgccaccttc ctgctgattg
    23821 actgcgctgc cttcgtcttt agacccatgg tctgcgaagg ggcgacacta tagctaccct
    23881 gatgagaaga gacaagccct tttctggtcc accagggccc aaagtggtag catatcatga
    23941 atattcctcc ctttgacgac gaatgcttat gtctatacag aacgaaatgc ctggttacaa
    24001 cgaaatgaac cagtatctga accaacaagg gacgggtctg accccagctg agatgcatgg
    24061 tttaatcagc gggatgatat gtggcggtaa cgatgacagc tcatggctac cgctacttca
    24121 cgacctgacg aacgaaggca tggctttcgg tcatgagctg gcacaggcac tgcgtaaaat
    24181 gcactctgcc accagcgatg ccctgcagga tgacggcttc ctttttcagc tttatctgcc
    24241 tgatggcgat gatgtcagcg ttttcgatcg ggctgatgca ttggcaggtt gggtcaatca
    24301 cttcctgctt ggtcttggcg ttacgcaacc gaagctggat aaagtgaccg gcgaaaccgg
    24361 tgaagctatc gacgatctgc gtaacattgc gcaactgggt tacgacgaag acgaagatca
    24421 ggaagagctt gaaatgtcgc ttgaagagat catcgaatac gttcgtgttg ccgcgctgtt
    24481 atgccacgac acctttactc atccgcaacc gaccgcgcca gaagtacaaa aaccgactct
    24541 acactaaaaa caaaaaacgt aaggagagtg ttatgagtga gatatcccgg caagagtttc
    24601 agcgtcgccg tcaggccctg gtggagcaaa tgcaacccgg cagcgccgcg ctgatttttg
    24661 ctgcaccaga agtaacacgt agcgccgaca gcgaataccc ctatcgtcag aacagtgact
    24721 tctggtactt caccggcttt aacgaaccgg aagcggtgct ggtgctgatt aaaagcgatg
    24781 acactcataa ccacagcgtt ctgtttaacc gcgttcgcga cctgacggcg gagatctggt
    24841 ttggccgtcg cttaggccag gatgccgcgc cagagaaact gggcgttgac cgcgcactgg
    24901 cattcagcga aatcaatcag caactttatc aactacttaa cggcctggat gtggtttacc
    24961 atgcccagga cgaatatgca tatgctgatg aaatcgtgaa cagtgcgctg gaaaaactgc
    25021 gtaaaggttc gcggcaaaat ctcaccgcac cggcaacgat gatcgactgg cgtcctgttg
    25081 ttcatgaaat gcgcctgttc aaatcgccag aagagattgc cgtactccgc cgcgcgggag
    25141 aaatcaccgc catggcacat acccgggcga tggaaaaatg ccgtccggga atgttcgagt
    25201 accatctgga aggcgaaatt caccacgaat ttaaccgcca cggtgcgcgc tatccgtcct
    25261 ataacaccat tgtcggcagc ggtgaaaacg gctgcattct gcactacacc gaaaacgagt
    25321 gtgaaatgcg cgacggcgac ctggtgttga ttgacgcggg ttgcgaatac aaaggttacg
    25381 ctggcgatat tacccgcacc ttcccggtca acggcaaatt cacccaggcc cagcgtgaaa
    25441 tctacgacat tgtgctggag tctctcgaaa ccagcctgcg cctgtatcgt ccgggaactt
    25501 ccattctgga agtcactggt gaagtggtgc gcatcatggt tagcggcctg gtaaaactcg
    25561 gcatcctgaa aggtgatgtt gatgaactga tcgctcagaa cgcccatcgt cctttcttta
    25621 tgcatggcct tagccactgg ttaggactgg atgtccatga cgttggcgtt tatggtcagg
    25681 atcgctcgcg cattctggaa ccggggatgg tactgaccgt agagcctggt ctgtatatcg
    25741 cgccggatgc ggatgtgcca gaacaatatc gcggtatcgg cattcgtatt gaagacgaca
    25801 ttgtgattac cgaaaccggt aacgaaaacc tcaccgccag cgtggtgaaa aagccggaag
    25861 aaatcgaagc gttgatggct gctgcgagaa agcaatgagc gtaatcatcg tcggtggcgg
    25921 catggcgggc gcgacgctgg cgctggctat ttcccggtta agtcacgggg cgctgccggt
    25981 acatttgatt gaagcgactg cgccagagtc acatgctcat ccgggctttg atggacgagc
    26041 gatagcgctg gcggcgggta cctgtcagca actggcgcgc atcggcgtct ggcaatctct
    26101 ggcggattgc gcaactgcca tcaccaccgt gcatgtcagc gatcgtggtc acgctggatt
    26161 tgtcaccctc gccgcagaag attaccaact ggcggcgctg ggacaggttg tcgaattgca
    26221 caatgtcggg caacggctgt ttgcattgct gcgtaaagca cctggcgtaa cgctgcattg
    26281 ccctgatcgc gtggctaacg ttgcccgtac tcagagtcac gttgaagtga cgctggagag
    26341 tggcgagacg ctgacgggcc gcgtgctggt agccgctgat ggcacccatt cagcgttagc
    26401 caccgcgtgc ggcgttgact ggcagcagga gccttacgaa caactggcag tgattgccaa
    26461 cgttgctact tctgttgcgc acgaaggccg cgcttttgaa cgcttcacgc aacatggccc
    26521 gctggcgatg ttgccgatgt ctgacggacg ctgttcgctg gtctggtgtc atccactgga
    26581 acggcgcgaa gaggtgctgt cgtggagtga cgagaagttt tgccgtgagc tccagtcggc
    26641 ctttggctgg cgacttggaa aaattaccca cgccggtaaa cgcagtgctt atccgctggc
    26701 gttaacccgc gccgccagag ctattaccca tcgtaccgtg ctggtgggca atgcggcgca
    26761 aaccctgcac ccgattgccg ggcaagggtt caacctcggt atgcgagatg tgatgagtct
    26821 tgcggaaacc ctgactcagg cgcaggagcg cggagaagac atgggggatt acggcgtatt
    26881 gtgccgttat cagcagcgtc gacagagcga tcgcgaagca accatcggcg tcacggacag
    26941 cctggtacat ctttttgcca accgttgggc accgctggtt gtcgggcgca acatcggact
    27001 gatgacgatg gaattattca ccccggcacg cgatgtgctg gcgcagcgca ccctcggttg
    27061 ggtggcgcgt tgaaggcggt tttaaggaat aaacaatgca aagtgttgat gtagccattg
    27121 ttggtggcgg catggtgggg ctggcggttg cctgcggctt acaggggagc ggcttacgcg
    27181 ttgccgtact ggagcagcgc gtaccggaac ctctggcggc ggatgcacca ccacaactgc
    27241 gcgtttcggc tatcaatgcc gccagcgaaa aattactcac ccgtcttggc gtctggcagg
    27301 acattctctc tcgtagggcc agttgttatc acggtatgga agtgtgggac aaagacagtt
    27361 ttggtcacat ttcgtttgac gatcaaagca tgggctatag ccatcttggg catatcgttg
    27421 aaaactcagt gattcactac gcgctgtgga acaaagcgca gcagtcgtca gatatcactc
    27481 tgttggcccc cgcagaatta cagcaggtcg cctggggaga aaatgaaact ttcctgacgc
    27541 tgaaagatgg cagcatgtta acggcgcgtc tggtgattgg cgcggacggt gctaattcct
    27601 ggttgcgcaa caaagcagat attccgctga ctttctggga ttatcagcat cacgcgctgg
    27661 tagcgaccat tcgcacggaa gaaccgcatg atgcggtggc gcggcaggtt ttccatggcg
    27721 aaggcattct ggccttttta ccgcttagcg atccgcatct ttgctcaatt gtctggtcac
    27781 tgtcgccaga ggaagcgcag cggatgcagc aggcaagtga agatgaattt aatcgcgcgt
    27841 taaatatcgc ttttgataat cgcctgggct tatgcaaggt tgagagcgcg cgtcaggtgt
    27901 tcccactgac ggggcgttat gcgcgccagt ttgccgcgca ccgtctggtg ctggtgggcg
    27961 acgccgcgca taccattcac ccgctggcgg ggcagggagt aaatctcggc tttatggatg
    28021 ctgcagagct gattgctgaa ctgaaacggt tgcatcgtca ggggaaagac atcgggcagt
    28081 acatttacct gcgccggtat gagcgtagcc gcaagcacag tgcggcgctg atgctggctg
    28141 gtatgcaggg attccgcgat ctgttttccg gtaccaatcc ggcgaaaaaa ctgctgcgtg
    28201 atattggttt gaaactggcc gacacgcttc ctggcgttaa accacaactt atccgccagg
    28261 caatgggatt aaacgatttg cctgaatggc tgcgttaaaa atttctcttc tgctgtttat
    28321 ttgataccca tcacactttc atctcccggt tttttcgccg ggagattttc ctcatttgaa
    28381 ataaactaat ttcacctccg ttttcgcatt atattttcta atgtcattat tttttgattt
    28441 agtgttt
//
LOCUS       DAJOSY010000054        28247 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJOSY010000054 DAJOSY010000000
VERSION     DAJOSY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.054"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            189..860
                     /locus_tag="OHN27_002439"
     CDS             189..860
                     /locus_tag="OHN27_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCQ2286858.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            870..2066
                     /locus_tag="OHN27_002440"
     CDS             870..2066
                     /locus_tag="OHN27_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCQ2286859.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2044..2625
                     /locus_tag="OHN27_002441"
     CDS             2044..2625
                     /locus_tag="OHN27_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCQ2286860.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2627..3400
                     /locus_tag="OHN27_002442"
     CDS             2627..3400
                     /locus_tag="OHN27_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCQ2286861.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3587..3862
                     /gene="frmR"
                     /locus_tag="OHN27_002443"
     CDS             3587..3862
                     /gene="frmR"
                     /locus_tag="OHN27_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCQ2286862.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3897..5006
                     /gene="frmA"
                     /locus_tag="OHN27_002444"
     CDS             3897..5006
                     /gene="frmA"
                     /locus_tag="OHN27_002444"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCQ2286863.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5098..5931
                     /gene="fghA"
                     /locus_tag="OHN27_002445"
     CDS             5098..5931
                     /gene="fghA"
                     /locus_tag="OHN27_002445"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCQ2286864.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMHHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLAENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6057..6596)
                     /locus_tag="OHN27_002446"
     CDS             complement(6057..6596)
                     /locus_tag="OHN27_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCQ2286865.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6698..7909)
                     /gene="mhpT"
                     /locus_tag="OHN27_002447"
     CDS             complement(6698..7909)
                     /gene="mhpT"
                     /locus_tag="OHN27_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCQ2286866.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVIAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8184..9197)
                     /gene="dmpG"
                     /locus_tag="OHN27_002448"
     CDS             complement(8184..9197)
                     /gene="dmpG"
                     /locus_tag="OHN27_002448"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCQ2286867.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVASSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9194..10144)
                     /gene="mhpF"
                     /locus_tag="OHN27_002449"
     CDS             complement(9194..10144)
                     /gene="mhpF"
                     /locus_tag="OHN27_002449"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCQ2286868.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10141..10950)
                     /gene="mhpD"
                     /locus_tag="OHN27_002450"
     CDS             complement(10141..10950)
                     /gene="mhpD"
                     /locus_tag="OHN27_002450"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCQ2286869.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10960..11826)
                     /gene="mhpC"
                     /locus_tag="OHN27_002451"
     CDS             complement(10960..11826)
                     /gene="mhpC"
                     /locus_tag="OHN27_002451"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCQ2286870.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11844..12788)
                     /gene="mhpB"
                     /locus_tag="OHN27_002452"
     CDS             complement(11844..12788)
                     /gene="mhpB"
                     /locus_tag="OHN27_002452"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCQ2286871.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12790..14454)
                     /locus_tag="OHN27_002453"
     CDS             complement(12790..14454)
                     /locus_tag="OHN27_002453"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HCQ2286872.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALMREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14531..15478
                     /gene="mhpR"
                     /locus_tag="OHN27_002454"
     CDS             14531..15478
                     /gene="mhpR"
                     /locus_tag="OHN27_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HCQ2286873.1"
                     /translation="MIFYCALSIGRVFSATIKTCSNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLASRPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            15555..16637
                     /gene="lacI"
                     /locus_tag="OHN27_002455"
     CDS             15555..16637
                     /gene="lacI"
                     /locus_tag="OHN27_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HCQ2286874.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16760..19834
                     /gene="lacZ"
                     /locus_tag="OHN27_002456"
     CDS             16760..19834
                     /gene="lacZ"
                     /locus_tag="OHN27_002456"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCQ2286875.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIKVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19886..21139
                     /gene="lacY"
                     /locus_tag="OHN27_002457"
     CDS             19886..21139
                     /gene="lacY"
                     /locus_tag="OHN27_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HCQ2286876.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21205..21816
                     /gene="lacA"
                     /locus_tag="OHN27_002458"
     CDS             21205..21816
                     /gene="lacA"
                     /locus_tag="OHN27_002458"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HCQ2286877.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
     gene            complement(21919..23100)
                     /gene="cynX"
                     /locus_tag="OHN27_002459"
     CDS             complement(21919..23100)
                     /gene="cynX"
                     /locus_tag="OHN27_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HCQ2286878.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPMQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPVRFPQLWVKE
                     A"
     gene            complement(23106..23576)
                     /gene="cynS"
                     /locus_tag="OHN27_002460"
     CDS             complement(23106..23576)
                     /gene="cynS"
                     /locus_tag="OHN27_002460"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HCQ2286879.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     EALLGQQALPADAARLVGAKLDLDEDSILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23607..24266)
                     /gene="cynT"
                     /locus_tag="OHN27_002461"
     CDS             complement(23607..24266)
                     /gene="cynT"
                     /locus_tag="OHN27_002461"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HCQ2286880.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24376..25275
                     /gene="cynR"
                     /locus_tag="OHN27_002462"
     CDS             24376..25275
                     /gene="cynR"
                     /locus_tag="OHN27_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HCQ2286881.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLRLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLNMALEECAVVGGNESR"
     gene            complement(25612..26895)
                     /gene="codA"
                     /locus_tag="OHN27_002463"
     CDS             complement(25612..26895)
                     /gene="codA"
                     /locus_tag="OHN27_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HCQ2286882.1"
                     /translation="MSNNALHTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPVTE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26885..28144)
                     /gene="codB"
                     /locus_tag="OHN27_002464"
     CDS             complement(26885..28144)
                     /gene="codB"
                     /locus_tag="OHN27_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HCQ2286883.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         6724 a         7676 c         7329 g         6518 t
ORIGIN      
        1 atacaaacac ttaacatatg aattgcatag aaacattacc cagtcgaacc cccgggtctg
       61 aaagcccttg cttttcagtt attagcaaca tttaacattt ataaattaac atctggctgc
      121 gccagagatt acgtctctga gtgcatcgtt gtgcttgtag tataataaat aaaatggagt
      181 gtcattaagt ggataaggtt ttagattcag ccctcctttc ttcggcaaat aaaagaaagg
      241 gtatccttgc tattggcgca catcctgacg atatagaatt aggctgtggc gcatcgcttg
      301 ctcgtcttgc gcaaaaagga atttatatcg cagccgtggt gatgactacc ggcaactctg
      361 gtacagatgg aataatcgat cgccatgaag aatcgcgcaa cgccctaaag atattagggt
      421 gccaccaaac tattcatctt aattttgctg acacccgcgc tcatttacag ctcaatgata
      481 tgatttccgc cctggaagac atcattaaaa atcaaattcc ttctgatgtt gaaatcatgc
      541 gggtatatac catgcatgat gccgaccgcc atcaggatca tctcgctgtt tatcaagctt
      601 caatggttgc ctgccgcact attccacaaa ttctcggcta cgaaacccca agtacctggc
      661 tttcatttat gcctcaggtt tttgaatccg ttaaagaaga atatttcaca gtcaaacttg
      721 ctgcattaaa aaaacataaa agccaggaac gacgcgatta tatgcgtcat gatcgcctgc
      781 gtgcagttgc acaatttcgc gggcaacagg tcaatagcga tctgggtgaa ggctttgtaa
      841 ttcataaaat gattctttga gcaaattgca tgaaaacctg gatatttatc tgtatgtcca
      901 tagcaatgtt gctatggttt ttaagtacgc taagacgtaa acccagtcaa aagaaaggct
      961 gtattgacgc cattatacct gcgtataacg aaggcccgtg tctggcgcag tcactggata
     1021 atctactgcg taacccttat ttttgccggg taatttgcgt taacgacggc tccacggaca
     1081 ataccgaagc ggtcatggcg gaagtcaaac gcaaatgggg cgaccgcttt gttgccgtca
     1141 cgcaaaaaaa taccggtaaa ggtggtgcgc tgatgaatgg cctcaattat gccacctgcg
     1201 accaggtttt tttaagtgat gccgacacct atgttccgcc cgatcaagac ggaatgggct
     1261 atatgctggc agaaattgag cgcggtgctg atgccgtagg cggcattccc tctactgcgt
     1321 tgaaaggcgc ggggctgtta ccgcacatcc gcgcgaccgt aaagttgccg atgattgtta
     1381 tgaagcgcac gctacagcag ctcctgggcg gcgcaccgtt tattatcagc ggtgcctgcg
     1441 ggatgttccg tactgatgta ttgcgtaagt tcggtttctc ggatcgtact aaagtcgaag
     1501 accttgatct cacctggaca ttagtggcaa acggctaccg tattcggcag gcgaatcgct
     1561 gcatcgtata cccacaggaa tgcaacagcc cgcgtgagga gtggcgtcgc tggcggcgtt
     1621 ggattgtggg atacgcggtc tgtatgcgcc tgcataaaag gcttttattt agccgcttcg
     1681 gtatcttcag tatatttcct atgctgttgg ttgtgcttta tggcgttggg atttatctca
     1741 ctacctggtt taatgaattc atcaccaccg ggccgcatgg tgtggtgttg gcaatgtttc
     1801 cgcttatctg ggtcggcgta gtttgtgtta ttggtgcttt tagcgcctgg tttcatcgtt
     1861 gctggttgtt ggtgccttta gcgccgcttt ccgttgtgta tgtattatta gcttatgcca
     1921 tctggattat ttatggactt attgcctttt ttactggacg cgaacctcag cgcgacaaac
     1981 ccacccgcta ttccgcactg gtggaagcgt caaccgctta ttcccaacct tctgtcacag
     2041 gaactgaaaa attatctgaa gcttaatgtt aaagagaaaa acattcagat tgcagaccag
     2101 gtaattattg atgaaactgc aggtgaagtt gttatcggcg cgaatacccg tatttgtcat
     2161 ggtgccgtta ttcagggtcc ggtagtgatt ggcgcaaact gcctgatagg taattatgct
     2221 tttattcgtc ctggcacaat aatcagcaat ggcgtaaaaa ttggttttgc caccgagatt
     2281 aaaaatgcgg ttattgaagc ggaagcaacg attggtccgc aatgttttat tgccgactcg
     2341 gtagttgcaa accaggcata tttaggcgca caagtacgta ccagtaatca tcgtctggat
     2401 gaacagcccg tgtctgttcg aactccagag ggaattatcg ctaccggatg cgataaatta
     2461 ggttgttata tcgggcagcg ttcacgcctt ggcgtacaag ttattatttt gcctgggcga
     2521 attatttccc cgaacacgca acttggcccg cgcgtgattg tagaacgtaa tttacctgcc
     2581 ggaacttatt cactccgaca agaacttatc cgtacaggag attaaaatga taaaacggac
     2641 gttattagcg gcggccattt ttagcgcatt gcccgcttat gccgggttaa cttccattac
     2701 cgcgggctac gattttaccg attattctgg cgatcatggc aaccgtaatt tagcgtatgc
     2761 tgaactggtg gcgaaagttg aaaacgcaac gctgcttttt aatctttccc aggggcgtcg
     2821 tgattatgaa actgaacatt tcaatgccac tcgcggtcag ggtgcggtct ggtataaatg
     2881 gaataactgg ctgacaaccc gaacgggtat tgcctttgcg gataatacgc cggtctttgc
     2941 ccgccaggat tttcgtcagg atattaacct ggccctgttg ccaaaaacgc ttttcacgac
     3001 cggttatcgc tacactaaat attacgatga tgtcgaagtc gatgcctggc aaggcggcgt
     3061 atcactctat actggcccgg tgatcaccag ctaccgctat acccattatg actccagcga
     3121 tgcaggtggt agttatagca atatgatttc cgtgcgtctg aatgacccgc gcggcactgg
     3181 ttatacgcaa ctatggctaa gccgcggaac aggcgcttac acctatgact ggacgccaga
     3241 aacacgctac ggcagcatga agagcgtcag tctgcaacgt attcaaccgc tgactgagca
     3301 acttaatctc gggctgacgg caggtaaagt gtggtacgac accccaaccg atgattataa
     3361 cggtctgcaa cttgcagccc atctgacctg gaaattctga ttccttctgc cgcccgctat
     3421 ccggggcggc cttccctgcc gattagcccc ccccctttcc tctttgtttt ccgaccacat
     3481 tcaccggata aattttattc tccagtgtta tatactatag gggggtatgc attgacatat
     3541 agcatacccc cctatagtat attgcgtgca gataatgagg tgcgaaatgc ccagtactcc
     3601 ggaagagaag aaaaaggtcc ttactcgagt tcgtcgtatt cgagggcaga ttgatgctct
     3661 ggaacggtcg ctggagggtg acgccgaatg ccgtgccata ctccaacaga tcgctgccgt
     3721 tcggggcgcg gctaatgggc tgatggcaga agtgcttgaa agccatatcc gggaaacgtt
     3781 tgaccgaaat gactgctaca gccgcgaagt cagccaatcc gttgacgaca ctattgaact
     3841 ggttcgagcc tatcttaaat agctgaatct attaccatat tgaggaagag cgagagatga
     3901 aatcacgtgc tgccgttgca tttgctcccg gtaaaccgct ggaaatcgtt gaaattgacg
     3961 ttgcaccacc gaaaaaaggt gaagtgctga ttaaagtcac ccataccggc gtttgccata
     4021 ccgacgcatt taccctctcc ggggatgacc cggaaggtgt attcccggtg gttctcggtc
     4081 acgaaggggc cggcgttgtg gttgaagtcg gtgaaggcgt aaccagcgtc aaacctggcg
     4141 accatgtgat cccgctttac accgcggagt gcggcgagtg tgagttctgt cgttctggca
     4201 aaactaacct ctgtgttgcg gttcgcgaaa cccagggtaa aggcttgatg ccagacggca
     4261 ccacccgttt ttcttacaac gggcagccgc tttatcacta catggggtgc tctacattca
     4321 gtgaatacac cgtggtcgcg gaagtgtctc tggcaaaaat taatccagaa gcaaaccatg
     4381 aacacgtctg cctgctgggc tgtggcgtga ccaccggtat tggcgcggtg cacaacacag
     4441 ctaaagtcca gccaggtgat tctgttgccg tgtttggtct tggcgcgatt ggtctggcag
     4501 tggttcaggg cgcgcgtcag gcgaaagcgg gtcggattat cgctattgat acgaacccga
     4561 agaaattcga tctggcgcgt cgcttcggtg ctaccgactg cattaacccg aatgactacg
     4621 acaaaccgat taaagatgtc ctgctggata tcaacaaatg gggtatcgac catacctttg
     4681 aatgcatcgg taacgtcaac gtgatgcgtg cggcgctgga aagtgcgcac cgcggctggg
     4741 gtcagtcagt gatcatcggg gtagcaggtg ccggtcagga aatctccacc cgaccattcc
     4801 agttggtcac cggtcgcgta tggaaaggtt ccgcgtttgg cggcgtgaaa ggtcgttccc
     4861 agttaccggg catggttgaa gatgcgatga aaggtgatat cgatctggaa ccgtttgtca
     4921 cgcataccat gagcctggat gaaattaatg acgccttcga cctgatgcat gaaggcaaat
     4981 ccattcgaac cgtaattcgt tactgatttc ccgcaggtat acccgtccac ttcagacggg
     5041 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5101 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5161 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5221 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5281 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5341 agtccgcgag gcagtcatgt cgcagatgct gaccgttacg atctcgggca aggtgccggg
     5401 ttttacctga acgcgacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5461 cgcaacgagc tgccggattt agtgatgcat cattttccgg caacggccaa aaagtctatc
     5521 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5581 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtgcc gtggggacag
     5641 caagcctttg ctgcatatct tgctgaaaat aaagatgcct ggttggatta cgacccggtg
     5701 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     5761 gatttttacg cagaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     5821 atcaagacgt taatccgtta tcacgagggt tatgatcaca gctattattt tgtctccagt
     5881 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     5941 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6001 gcctgataag acgcgcatag cgtcgcatca ggcatctgcg cacgacttat ctatgtttac
     6061 cacatcaaat catcaggcac tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg
     6121 gcactgagcg cgctgtgcaa cacaatactg ctggcatcac gctgagcaat tttatcggca
     6181 acactggcgg gaataatggt gtattcacct tcgctattgt tatcgaccaa caagcgggca
     6241 atcgccagac gaccattaat caactgcgcc tgtgtgagct tatcgacaaa aatcttctta
     6301 atcagattgc cgtcagtgaa gttaaaacca atatcgccat tggcaatggt gattcggttc
     6361 atttcaatga gctgcttcac ctgggcttta tattctttcg ccaacgccgc ttgtttttgc
     6421 tgttcgctaa gctgtttatc acgctcaagc tgtgcctttt tattttcttc taccgcagcc
     6481 cgagcttcac gcgcctgaac gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt
     6541 ttgctggtga ctaatcctgc tttgagcaac tgctcttgta aggtaagttt tgccatcttt
     6601 gtttccaaac ccgctgaata atttttagcg agtatacctg taaacgccgt gggtgtattg
     6661 ggggcaagtc cctttcgccc ctgcttaata aggcgcgtca ggcatcggca cacggctgca
     6721 ttcttgatct ccgactcatc aaaataaaca ccgccaaccc agcaacaaga atacccggtg
     6781 cagaagcggc aattacgccg accgtgccag tgcctaatgc cagcattttc ccggccagta
     6841 acggaccgct catagccccc agacgcccta ccgccacggc tgttcccaca cctgttgcgc
     6901 ggatctgcga actgtaaaac aacggtgcca gggcatacaa aacgctttgc ccacctgtcg
     6961 caaacaaccc cgcgacaaat cccgccagca acataccgtt aaatgacgac accgttccaa
     7021 gcgccagcag cgaagctaac atgccgctat aaatcagtag cgacatggtt actggacgca
     7081 gcttatccat caatgcgccc aacattaacg tcccgcttgc cgcccccatt tgcagggcaa
     7141 acatcacccc tgccgcctgc gatggctgga atccttgctc caccaaaagt agcggtagcc
     7201 agttgatcaa catataaacc accagcagag tgaagaaata acacaaccac agcagcagcg
     7261 tcgcggttgc cgtttctggc gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc
     7321 cagcgaaaac cgctgactcc ggcagccagc gcattaatag cggcaccaga atcaacggca
     7381 ccacaccacc tacccaaaac accgtttgcc atgctaagtt tgcccccgcg aaacccagtg
     7441 tcgccgccag cgccgcgcca atgggaacac cgcaatacat caggctcact gccgtcccac
     7501 gaaaacgtgg acccgcggct tcagacgtca gggcgataag attcggcaac gccgccccca
     7561 gcccgacacc ggtcatcagc cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg
     7621 ttgccagtga gaacaaacca aacagcgcaa ctgagccaat caaaatacgc ttacgaccat
     7681 aacggtctgc cagcatcccg ccaaccaacg cgccgggtag caaaccgagt attccggcgc
     7741 taaatatcca gcccatttgc attttatcga gtgcgaaagc ctgggcgatg ccacccgccg
     7801 caatgccagc cgcctgaaga tccagccctt ccatcagagc gaccaaaaaa caaagcccga
     7861 tggtcagcat caggcgggat gaagatgatg aaggggtacg agtcgacata agatgaacct
     7921 cgttgcgagt gcaggtgaat cgcgactgat acattgccag ccgcgtaatc agaactattt
     7981 aatgactgcc tacgggcgaa atgtaggccg gacaagacgt ttatcgcatc cggcagtcgt
     8041 gtgcagatgc ctgatgcgac gctaacgcgt cttatcaggc ctacaaattc tcacgccacc
     8101 cgtaggccgg ataaggtgtt tacgccgcat ccggcagtcg tgctgggatg ggcatacaaa
     8161 atgcacgttc tcggcagtca tcattatttg ttgttgcgca gatccagcgc cacgtcaacg
     8221 atcatatcct cctggccgcc aaccatccgg cgtttgccca gctcaacgag aatatccacc
     8281 gcacttaagc cataacgcgc cgccgccgtt tcacagtgac gcaggaagct cgagtaaaca
     8341 ccagcgtatc ccagcgccag cgtttcgcga tcgactcgta ccggtcgatc ctgcaacgga
     8401 cgcaccaggt cgtcggcggc atccattaac gcatagagat cggtcccatg ctgccagccc
     8461 agtttatccg cggcggcaat aaacacttcc agcggtgcgt tacctgcgcc cgcgcccatt
     8521 cccgcgaggc tggcgtcgat tcggtcgcag ccctcttcca ccgccgcgat agagctcgcc
     8581 acgccaagac tcaggttatg gtgagcgtgc atgccagttt gcgtttctgg tttcagctct
     8641 gctttcaggg cgcggaaacg gtcacggata tcgctcatgt tcatcgcacc gccagaatcc
     8701 accacataaa tacaggtcgc accgtagcct tccatcagct ttgcctgctt ggcgagattc
     8761 tccggcgtgg tcatatggct catcatcaga aaaccaacgg tgtccattcc gagctcgcgg
     8821 gcatactgaa tatgctgggc ggaaacatca gcttcggtac agtgcgttgc cacacgaacc
     8881 acccgcgcgc cagcctgcca ggcatttttc agatcgtgaa tagtgccgat tcctggcagc
     8941 aacaacgtcg cgattttggc gtgcttcacc acatccgccg ccgcttcaat ccattcaagg
     9001 tcgctatgtg cgccgaaacc atagttaaag ctggaacctt gcaaaccgtc gccgtgggcc
     9061 acttcaatcg aatccacgcg ggcatcgtcc agtgctttgg caatctggcg aacgttttcc
     9121 agcgaatact gatgacgaat ggcgtgcata ccgtcacgca atgtgacgtc cgagatataa
     9181 agttttttac cgttcatgcc gcttctcctg ccttgcgcgc cagtgactgg gccatttttt
     9241 ccgctgtcgc cagcgcactg gaagtcataa tatcgaggtt gcccgcatag gcaggcagat
     9301 aatgcgctgc gccttcgact tccagccaga ccgctgtttt cagtccggag aattgcccca
     9361 cgcccggtaa attgaccggt ttatcctgcg ggataacttc aaactgcacg cgctgtttca
     9421 ggcgataacc cggtacgtaa gcctgcaccg cctcagccat ttcattgatt gaggcttcga
     9481 tatcatcttg tgaagcttcg tcgctcaata catacaccgt gtcacgcatc atcaacggtg
     9541 gctctgctgg gttaagcaca ataatcgcct tcccttttgc cgcgccgccc accacttcaa
     9601 tggctcggga agtggtttcc gtaaattcat cgatattggc acgcgtgcca ggtccggcag
     9661 atttactggc gatagaagcg ataatttcgg cgtaatgaac acgcgccacg cgtgaaactg
     9721 ccgccaccat tggaatggtg gcctggccgc cgcaggtgac catgttgacg ttcagttgat
     9781 cgacgttcgc ctcgaggtta accaccggca cgcagtaagg gccgatggca gcaggcgtca
     9841 ggtcaattaa gcgaatatcc ggtttcgctt cgcgtaaagc ggcatcgttt ttcacatgcg
     9901 cgcccgcgct ggtcgcatca aatacaatgt cgatatcagc aaattcaggc atgttcatca
     9961 gcccgatcac cccttcatgg gtggtggcga cgcccatacg tctggcacgc gccagaccgt
    10021 cggactgagg atcaatgccg accatcaccg ccatctccag atgctgaccg tgacgcaaaa
    10081 ttttaatcat cagatcggtg ccaatgttgc cagaaccgat aatggcgact ttacgcttac
    10141 tcatgacaga cttccttttg gggctgcgct tgaaaatgtc gcagcaactg aacctatgcc
    10201 ttcaatatgg gcttcaaaac gatcgcccgc attcaccgcc accatcgggc ctaatgcccc
    10261 ggtaagaatg atatctccgg cgcgcagcgg ttcacccaga ctggccattt tgcgtgccag
    10321 ccagacggcg gcattaagcg gatgtcccag gcattcgctg ccgcgcccgc tggaaacctc
    10381 ttcgttatta cgcgtcatct tcatggcgca gtttttcagg tctaaccccg ccggacgttg
    10441 cgccggaccg ccgatgacat acaccccgca ggaagcgtta tctgccacgg tatcgacaaa
    10501 ctgaatcgac cagtcgcgaa tgcggctccc caccacctcc agcgccggaa gtacccattc
    10561 aatggcgtta tacaattcgt cgaaggtgat atcagttgcg ggcaaatcgc ggttcaacac
    10621 cagtgcgatc tccgcttcaa tgcggggttg cagaacacgg gaaaaaggaa tgatttcgtt
    10681 atcgccataa cacatgtcgg caaataacgt cccaaaatcc ggttgatcaa cgcccagttg
    10741 ttgttgcact ttcggatgtg tcaggcccac tttacgccct accacgcgac gcccctgcgc
    10801 aacgtcatgt tgcacattta tgtgctgaat ggcgtaagcc gcttcagcgt tatcgatacc
    10861 aatcagatcg cgcagcggtg caatcgcttc gccctgctct gcggcgcggc gtaaatccgc
    10921 cgccagttgc tcaagagtat gcttcgtcat gaccattcct taagggcgtg cgaggaaatt
    10981 cagcaccagt tgattgaaag cgtcggcatg ttcccactgc gcccagtgac cgcagtcgcg
    11041 gaagatatgc agttcagaac cggcaatgcc ggacagcaga cgcagacccg catccatcgg
    11101 cacaaagcgg tcgttgcgcc cccagacaat cagggtttgc gctttgattt ccgccagacg
    11161 tgggccaaaa tccgggaact gtttcggatt agcttccaag ctcttaacga agttttccag
    11221 gtgatcgcgg cgcgacagca tattattcag gcgcgcttca aacagggcgt cggtcaaatc
    11281 gctggtatca aaaacgaaga tatccatcat cagtttcagg ttttcgatag tcggctgacg
    11341 ataaagctga ttcagtcgct taataccttc ggttggcatc ggcgtaaaca aactcatgcc
    11401 gcccgtaccg ccgcccatca gcaccagttt gccgacgcgc tccggccagt taagggtgaa
    11461 cgccacagaa ctatggccgc ccatcgagtt gcccaacagg tggattttgg cgatatccag
    11521 ttgatccacc acgcttttca ggattcgtgc attaagatcc gatcgtgaac cactattaac
    11581 gatcgaatcg ctcttgcccc aacccggaca atccagcagg atcacccgat agcccgcctc
    11641 taccagcgga tcgatattgc ggctgaaatt cgcccagcca gtagcacccg ggccggaacc
    11701 atgcagcagg acaacggttt cgtcgccttg tccgcagtca ttaaaatgga tgcgcagcgt
    11761 tttacccgct tcttctacat tcagaaaacg gctggtggcg gcttcggttt gtggctgata
    11821 actcatcatc ttctcctgca tattcagttc tctgttctgg cgcttaacga gccaaatccg
    11881 gcaatccact ccgggattgg gcggtaataa cgcccttcgc tacgccagtt gccaaatgca
    11941 gaaatagcgg caaaagcggc gacccaggtt ttgatttcat gtgtcgactt tccggcaatg
    12001 gcggaaagct cttcgttact gacggcatcc agttcctgta tgcgtccctg ctccagcaaa
    12061 gtcatgaact ggttatccca aatcgggttg agcggatgca gagttctctg atcctcaaca
    12121 aacttctcag cggcgctaat caccctttgc tgacgcaatt cgcgctcact ggcgggtaaa
    12181 tctttcccgc tccccaacag acggtcgcgc atatgggcat cggctttcgc cagttcgggc
    12241 accggcggct gatgggaaag cccaccggaa cccaggaaca gcacgcgttt attgagagtg
    12301 ctggtgaaac gtccaatggc ttcacccaac atgcgggtac gctggaaacc gggcagcggc
    12361 gtggcgacac cgttgatgaa cacaggcaga actggcacct tatccagccc accgagcagg
    12421 aactccagcg gctgggcgaa cccgtggtcc acctgcatac agtaagaaac ggcaagatcg
    12481 atcccgctct tcatgacagc atgcgcacag gcctccgcca gctccacagg cacgggcagc
    12541 tctcctgccg cactgccgaa atcaccaatt gccgtcgctc caacgcctaa acagaacggt
    12601 ggcatcacgt catagaaaaa gccgttgtag tgatctggcg caaacagcac caccagttca
    12661 ggggagaaag ccgcaatacg ctcgcgggcg ctggcaatca cgccattgac ctcatcgagt
    12721 acctcttgcg ccgggtcgac ataccccacc aacggcgagt gggaaagaca gtgaagataa
    12781 gcgtgcatat caggctacct tttcgacaga aacgtcggca tcagggcggg tcagcgtcat
    12841 caccgacgcc agtttattca gcgtattgcc cagggtttgc ggaatggcgg tggcggcaac
    12901 aaagcgatcc gggcgcatca ccaccagcga agcattgtgt tgcgcgaacc agctacgcag
    12961 acgaccttgc gtatcgccca cgcgtagcac gccgtcgtgg ttatcctgtg cggtatgaat
    13021 ttgcacttcc ggcaccacct gaatgaagcg tgtgcccaac gcgcgccact gctggatttg
    13081 ctcgtcgctc atcccccaca gtggattgca tccccagcca attaccgcga agttcgcgcc
    13141 gatcgcgtta tcgagcagcg tcacgtcgcc gttttccagc gtgactttcg gctgaataaa
    13201 catcttgccg accggagagt gcttcgcttc gccctcacgc atcagcgcac cgccgtaata
    13261 ttgcggcatc ggcttgaagc gcatttcgag gaagtagcgt tttactggcg gcagataatt
    13321 caacagccag gaaacgccgt cacgtaacgt accctgccag cgtttcggcg gagccagcac
    13381 gttgcccgcc gtcacggaca ggtcaatcat cgctttggcg tgatcgcgac gttcttgttg
    13441 ataggtatcg agcagcgcat cgcgggcttt cccctggata accaacgcca gtttccatgc
    13501 gaggttaaag gcgtcgcgca taccactgtt atagccctgc ccctgccata ccggcatgat
    13561 gtgcgcggca tcgcccgcca gcagtacgcg atcaatacgg aaacgttgcg ccagtcgcgc
    13621 gttgtgggtg tagacacgct ggcgaatcaa ttcaacattg tccggattag gcagcacttt
    13681 gcttaacagc ttgcgcatat tttgcggctc acgcagctgc tcttcggttt ctcccggcat
    13741 caccataaat tcaaagcgac gtaccgcatg aggcagcgcg gcagaaacat acgggcgcac
    13801 cggatcgcaa cacaaataga tatgcggcgt acttaacgga tcgttggcga tatctaccac
    13861 aatccactga tttggcgcag ttttaccttc aaacggcaca ttcagagtgc gacggacaaa
    13921 acttgctcca ccgtcacagg ctaccagcca ctgggctttg accgtttccc gctgcccttc
    13981 tgccgttttc aggtgcaagg tcacttcgtc attttgctgg ctgaaggcct ccagctcgcg
    14041 ggcaaacaag cagcgcacat tcggaaaacg cgacaaccct tccagcatca ccgcatcgac
    14101 ctgtggctga ataaaggcgt tacggcgcgg ccagccaaat tcatcggtca ttggctgaat
    14161 atcagcaaaa cagcggcctt ttggggtgag aaaacgcatc gcgtgccacg gtgtagtgtg
    14221 cggcagaaca ttctcgacca ggccgaccga ctgcatggtg cgcagcgcct cgtcatcaat
    14281 accaatcgca cgcgggtagt cgatcaactt atcgagtttc tccaccacca gcacgtcaat
    14341 gcccatctga ccgagatagt tcgccatcat cagcccaacc ggaccggcac cagcgatcgc
    14401 cacctgaacg ctatggttaa cagcaggctg gatgtcaggg tgttgtattg ccatttcagt
    14461 acctcacgac tcggacaaaa tgccgttgcg cgcacagtac agcgcaactt attttgttaa
    14521 aaacatgtaa atgatttttt attgtgcgct cagtatagga agggtgtttt cggctacaat
    14581 caaaacatgc tcgaatgtgc accaggtgca caacgttgtt ttaactatag aaatgtcaat
    14641 taatatgcag aacaatgagc agacggaata caaaaccgtg cgcggcttaa cccgcggtct
    14701 aatgttatta aatatgttaa ataaacttga tggcggtgcc agcgtcgggc tgctggcgga
    14761 actcagcggc ctgcatcgca ccactgtgcg gcgactgctg gagacgctgc aggaagaggg
    14821 atatgtccgc cgtagcccct ccgatgacag ttttcgtctg accatcaaag tgcggcaatt
    14881 aagcgaagga tttcgtgacg aacagtggat ttctgcactg gcggccccac tgctgggcga
    14941 tctgttgcgc gaagtggtat ggccgacaga tgtgtccacg ctggatgttg atgcaatggt
    15001 ggtacgcgaa accacccacc gtttcagccg cttatctttt caccgggcaa tggtcgggcg
    15061 acgtttgccg cttctgaaaa ccgcctcggg cctgacctgg ctggcctttt gcccggaaca
    15121 agaacgcaag gaattaatcg agatgttagc ctcccgcccc ggtgatgact atcaactggc
    15181 acgagaaccg ttaaagctgc aagccattct ggcgcgcgcg cgcaaagagg gttacggaca
    15241 gaactaccgc ggctgggatc aggaggagaa gatcgcctct atcgccgtac cgctgcgcag
    15301 tgaacaacgg gtgattggct gtctgaatct ggtgtatatg gcgagcgcaa tgaccattga
    15361 acaggccgcg gaaaagcatc ttccggcgct acaacgggta gcaaaacaga tcgaagaagg
    15421 ggttgaatcg caggctattc tggtagccgg aaggcgaagc ggcgtgcatt tacgttgaca
    15481 ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat
    15541 tcagggtggt gaatgtgaaa ccagtaacgc tatacgatgt cgcagagtat gccggtgtct
    15601 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
    15661 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
    15721 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
    15781 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
    15841 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg
    15901 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
    15961 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
    16021 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg
    16081 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
    16141 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac
    16201 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg
    16261 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg
    16321 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata
    16381 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc
    16441 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
    16501 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa
    16561 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
    16621 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    16681 caggctttac actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa
    16741 tttcacacag gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg
    16801 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
    16861 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
    16921 cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg
    16981 gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca
    17041 cggttacgat gcgcccatct acaccaacgt gacctatccc attacggtca atccgccgtt
    17101 tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg
    17161 gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg
    17221 gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct
    17281 gagcgcattt ttacgcgccg gaaaaaaccg cctcgcggtg atggtgctgc gctggagtga
    17341 cggtagttat ctggaagatc aggatatgtg gcgaatgagc ggcattttcc gtgacgtctc
    17401 gttgctgcac aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga
    17461 tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta
    17521 cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc
    17581 tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct
    17641 gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt
    17701 tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg
    17761 cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg
    17821 cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt
    17881 gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc
    17941 gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc
    18001 caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct
    18061 accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt
    18121 gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg
    18181 ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac
    18241 cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc
    18301 ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct
    18361 gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg
    18421 gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca
    18481 gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg
    18541 cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc
    18601 gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg
    18661 gcaaaccatc aaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca
    18721 ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc
    18781 tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca
    18841 actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccggaca
    18901 catcagcgcc tggcagcagt ggcgtctggc tgaaaacctc agcgtgacac tccccgccgc
    18961 gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa
    19021 taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa
    19081 aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat
    19141 tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc
    19201 gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc
    19261 ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg
    19321 gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc
    19381 gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga
    19441 gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc
    19501 ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag
    19561 cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg
    19621 cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg
    19681 ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat
    19741 tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccagctga gcgccggtcg
    19801 ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc
    19861 cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact tttggatgtt
    19921 cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt ttttcccgat
    19981 ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt ttgccgctat
    20041 ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca aactcgggct
    20101 gcgcaaatac ctgctgtgga ttattaccgg catgttagtg atgtttgcgc cgttctttat
    20161 ttttatcttc gggccactgt tacaatacaa cattttagta ggatcgattg ttggtggtat
    20221 ttatctaggc ttttgtttta acgccggtgc gccagcagta gaggcattta ttgagaaagt
    20281 cagccgtcgc agtaatttcg aatttggtcg cgcgcggatg tttggctgtg ttggctgggc
    20341 gctgtgtgcc tcgattgtcg gcatcatgtt caccatcaat aatcagtttg ttttctggct
    20401 gggctctggc tgtgcactca tcctcgccgt tttactcttt ttcgccaaaa cggatgcgcc
    20461 ctcttctgcc acggttgcca atgcggtagg tgccaaccat tcggcattta gccttaagct
    20521 ggcactggaa ctgttcagac agccaaaact gtggtttttg tcactgtatg ttattggcgt
    20581 ttcctgcacc tacgatgttt ttgaccaaca gtttgctaat ttctttactt cgttctttgc
    20641 taccggtgaa cagggtacgc gggtatttgg ctacgtaacg acaatgggcg aattacttaa
    20701 cgcctcgatt atgttctttg cgccactgat cattaatcgc atcggtggga aaaacgccct
    20761 gctgctggct ggcactatta tgtctgtacg tattattggc tcatcgttcg ccacctcagc
    20821 gctggaagtg gttattctga aaacgctgca tatgtttgaa gtaccgttcc tgctggtggg
    20881 ctgctttaaa tatattacca gccagtttga agtgcgtttt tcagcgacga tttatctggt
    20941 ctgtttctgc ttctttaagc aactggcgat gatttttatg tctgtactgg cgggcaatat
    21001 gtatgaaagc atcggtttcc agggcgctta tctggtgctg ggtctggtgg cgctgggctt
    21061 caccttaatt tccgtgttca cgcttagcgg ccccggcccg ctttccctgc tgcgtcgtca
    21121 ggtgaatgaa gtcgcttaag caatcaatgt cggatgcggc gcgagcgcct tatccgacca
    21181 acatatcata acggagtgat cgcattgaac atgccaatga ccgaaagaat aagagcaggc
    21241 aagctattta ccgatatgtg cgaaggctta ccggaaaaaa gacttcgtgg gaaaacgtta
    21301 atgtatgagt ttaatcactc gcatccatca gaagttgaaa aaagagaaag cctgattaaa
    21361 gaaatgtttg ccacggtagg ggaaaacgcc tgggtagaac cgcctgtcta tttctcttac
    21421 ggttccaaca tccatatagg ccgcaatttt tatgcaaatt tcaatttaac cattgtcgat
    21481 gactacacgg taacaatcgg tgataacgta ctgattgcac ccaacgttac tctttccgtt
    21541 acgggacacc ctgtacacca tgaattgaga aaaaacggcg agatgtactc ttttccgata
    21601 acgattggca ataacgtctg gatcggaagt catgtggtta ttaatccagg cgtcaccatc
    21661 ggggataatt ctgttattgg cgcgggtagt atcgtcacaa aagacattcc accaaacgtc
    21721 gtggcggctg gcgttccttg tcgggttatt cgcgaaataa acgaccggga taagcactat
    21781 tatttcaaag attataaagt tgaatcgtca gtttaaatta taaaaattgc ctgatacgct
    21841 gcgcttatca ggtctacaag ttcagcgatc tacattagcc gcatccggca tgaacaaagc
    21901 gcaggaacaa gcgtcgcatc atgcctcttt gacccacagc tgcggaaaac gtactggtgc
    21961 aaaacgcagg gttatgatca tcagcccaac gacgcacagc gcatgaaatg cccagtccat
    22021 caggtaattg ccgctgatac tacgcagcac gccagaaaac cacggggcaa gcccggcgat
    22081 gataaaaccg attccctgca taaacgccac cagcttgcca gcaatagccg gttgcacaga
    22141 gtgatcgagc gccagcagca aacagagcgg aaacgcgccg cccagaccta acccacacac
    22201 catcgcccac aataccggca attgcatcgg cagccagata aagccgcaga accccaccag
    22261 ttgtaacacc agcgccagca ttaacagttt gcgccgatcc tgatggcgag ccatagcagg
    22321 catcagcaaa gctcctgcgg cttgcccaag cgtcatcaat gccagtaagg aaccgctgta
    22381 ctgcgcgctg gcaccaatct caatatagaa agcgggtaac caggcaatca ggctggcgta
    22441 accgccgtta atcagaccga agtaaacacc cagcgtccac gcgcggggag tgaataccac
    22501 gcgaaccgga gtggttgttg tcttgtggga agaggcgacc tcgcgggcgc tttgccacca
    22561 ccaggcaaag agcgcaacaa cggcaggcag cgcccaccag gcgagtgttt gataccaggt
    22621 ttcgctatgt tgaactaacc agggcgttat ggcggcacca agcccaccgc cgcccatcag
    22681 agccgcggac cacagcccca tcaccagtgg cgtgcgctgc tgaaaccgcc gtttaatcac
    22741 cgaaggcatc accgcctgaa tgatgccgat ccccacccca ccaagcagtg cgctgctaag
    22801 cagcagcgca ctttgggggt aaagctcacg catcaatgca ccgacggcaa tcagcaacag
    22861 actgatggcg acactgcgac gttcgctgac atgctgatga agccagcttc cggccagcgc
    22921 cagcccgccc atggtaacca ccggcagagc ggtcaacagg gcagccacgc taaagctcat
    22981 tccgctcgcc tggcgcaatt gcggtagcag tggcccgacg gaggtgagca gtggtcgcat
    23041 attaagacca atcagcacca gtaccagcag catcgtgaac gtcttttgat ggttgcgcat
    23101 ggctgtcaga acggtttggt cggcagatat ttaccatcta aggtgatgac cgcacgttcg
    23161 ccaccttccg ggtccgccac tttcttaacg tcgagtttga agttaatcgc gctaataatg
    23221 ccatcgccaa atttctcatg aaccaacgct ttcagggttg taccgtacac ctgcaacatt
    23281 tcatagaaac gatacatcgt tgggtcagtt ggaatacggt catcaatgca gccacgcagt
    23341 ggaatcatct gcaacagtag aatggagtct tcgtcgagat ccagcttcgc cccgaccagg
    23401 cgggcggcgt cggcaggaag cgcctgctga cccagcaaag cctcggttac aaaggcttct
    23461 gccagaccgg taccgtcggc aatctcggca aatgagagat cttttttagc tttgctgagc
    23521 aaaatggcat cggcaagatc aagacgaata ttgcggttaa tttgtgactg aatcatggtg
    23581 gatctcctga tggtttaaaa ataaggttac gctgcggtag gttggcgtag cggtatggca
    23641 caaacgcgag gattagcggc cagtggcaca aactggcggg ttgcgccgtc aaaagctgcg
    23701 atgctgccgc tttcaatgtc gtagacccag ccgtgcaggg cgatccgccc ctcttcgagc
    23761 gccaggcgca ccgatggatg agtttgcaaa ttagccaact gagcaatgac gttttcacgt
    23821 accatcgccg cagcttttga cggtaaatcg gaatgcgggc gcgcctcatt aacgacgcgg
    23881 gctgaatcgg cataacgcag ccagtgggag acggcaggca tatggtccat acactgacag
    23941 ctggcaatgg cggtcatcgc gccacagttg gaatgaccac aaatcacaat gtcagatacc
    24001 cgaagcgcag cgacggcata ctccaccgaa gcagaaacgc caccgggttc cggcccgtag
    24061 gaagggacaa tattacctgc gttgcgaata acgaacagat cgccaggctc acgttgcgtc
    24121 accagctcag ggaccagacg gctgtcggag caggagataa aaagggtgcg cgggctttgc
    24181 tgtgtcgcca gctgtttaaa taaggcttcc cgcttcggaa atgcctcgcg ctggaattta
    24241 aggaatccat caataatctc tttcaccgtt aacctctgtc tgtctctgga atgagaggca
    24301 gactactcgc cgattgtcat aaggtaaaag tctcatttat gatgagttct attggattta
    24361 cttataggtt ggcgaatgct ctctcgacat atcaactatt ttcttgccgt ggctgaacat
    24421 ggcagcttta cccgtgccgc cagtgcgttg cacgtctccc agcctgcgct ttcccagcag
    24481 attcgccagt tagaggagag tttaggcgtg ccgctgtttg accgtagcgg gcgaacgatt
    24541 cgtctcactg atgcaggaga agtctggcga cagtacgcca gccgggcgtt acaggaactg
    24601 ggggcgggta aacgggcgat tcatgatgtt gccgatctga cgcgaggatc gctgcgtatc
    24661 gccgtcactc ccacctttac aagctacttt atcggccctt taatggcgga tttctatgcg
    24721 cgctaccccg gcatcacact ccggctacag gaaatgtcgc aggagaaaat cgaagatctg
    24781 ctttgccgcg acgagttgga cgttgggatt gccttcgcgc ctgtgcattc gccggagctg
    24841 gaggcaattc ctttactgac agaaagttta gcgttagtcg tggcgcaaca tcatccgctg
    24901 gctgcctgtg aacaggtggc gttgagtcgc ttgcatgatg aaaaactggt actgctcagc
    24961 gcggaatttg ccaccagaga gcaaattgac cactactgcg agaaagcggg gctgcatcca
    25021 caggtggtca ttgaggcgaa ctcaattagc gcggttctgg agctgattcg ccgcacttcc
    25081 ctttccacat tgttaccagc agcgattgcc acacaacatg acgggcttaa agcgatttct
    25141 cttgccccgc cactactgga gagaacggcg gttttgttgc ggcggaaaaa tagctggcag
    25201 acagccgccg cgaaggcatt tttgaacatg gcgttggagg aatgcgcggt tgttggcggg
    25261 aatgaatcac ggtagccgct gacacaggtc tgcggctgga attgcctgat gcgctacgct
    25321 tatcaggcct acgagttcag tgctctacgt aggccggata aggcgttcac gccgcatccg
    25381 gcataaacta cagctggcag actgccgctg cgaaggcatt tttgcacatg gcgttggagg
    25441 aatgcgcgga tgttggcgga aatgaatcac ggtagccgct gacacagatc agcggctgga
    25501 attgcctgat gcgctacgct tatcaggcct acgagttcag tgctctacgt aggccggata
    25561 aggcgttcac gccgcatccg gcataaacta agctcgctgt aacccagtcg ttcaacgttt
    25621 gtaatcgatg gcttctggct gctccagata tacggtggtt tgtgccggtt gtgtgctggc
    25681 aatcaccttg ccgccacgta ccgaatagcg taccggaacc tgacggcgca gcgcatcaaa
    25741 tccattttca gccggcagga taatcaggtt tgcgctgttt ccggcggcaa tgccgtaatc
    25801 ctgcaaattc aacgttctgg cactgtggtg ggtgattaaa ttcaggccat cgttaatctg
    25861 cccgtagccc atcaactggc aaacatgcag ccccatatgc agcacttgca gcatattcgc
    25921 cgttcccagc ggataccacg gatcgaagac atcatcgtga ccaaagcaga cgttaatgcc
    25981 ggactccagc atctctttaa cgcgcgtgat gccgcgacgt tttggatacg tatcgaaacg
    26041 tccttgcaga tgaatattga ccagcgggtt ggcgacaaag ttaataccgg acattttcag
    26101 caagcggaac aggcgtgagg tatacgcccc gttataggag tgcattgccg tggtgtggct
    26161 ggcggtgact cgcgcgccca tgccttcacg gtgcgccagg gcagcaacag tttcgacaaa
    26221 gcgcgactgc tcgtcatcga tctcatcaca gtgaacgtcg atgagacggt cgtatttttg
    26281 cgccagggcg aaggttttat gcagcgactc cacgccgtat tcacgggtaa attcaaaatg
    26341 cggaatcgcc cccactacat ctgcccctaa gcgtaacgcc tcttccagca acgcttcacc
    26401 gttgggatac gacaaaatcc cttcctgagg gaaggcgacg atttgcagat caatccacgg
    26461 cgcgacttcc tgcttcactt ccagcattgc tttcagcgca gtcagcgttg catccgaaac
    26521 atcgacatgg gtacgcacat gctgaatgcc gttggcaatc tgccatttca gcgtttgcca
    26581 tgcgcgttgt ttcacatcgt catgggttaa taacgctttg cgctcggccc agcgttcaat
    26641 gccttcgaac agcgtgccgg actgattcca gttcggttgt ccggcggttt gcgtggtgtc
    26701 caggtgaata tgcggctcca caaatggcgg tataactaaa ccttgttcgg catcaaggct
    26761 gttttcagtt acgggcatca cgccggattg cgcatcaatg gcgctgattt ttccgtcctg
    26821 cagatgaatc tgccacagcc cctctttgcc gggtaaccgg gcgttaataa ttgtatgtaa
    26881 agcgttattc gacactgtta gcctccacat gcgtcattgc tgctgtcgtt ttacgattca
    26941 aaatcgggtt aaggatcaga tagctcagcg cgccacctaa taccgcgttg accggaacaa
    27001 ttcccggtaa ccagtggcct gcggcaatcc ccagcgcgac cgccagaatc gccacccaat
    27061 tgacactcat catacgcgtg gtcgcaaagt gctcatagcg gcgacggttc atcagataat
    27121 cagcgatgat cacgccaccc accggaggaa tagctgccga aaggaaggtc agccagccga
    27181 caaaattgtt atacagccat aatgcgcaga ccgtaccgat aataccgttg atcaccgaaa
    27241 gcgttttgct cgacataccg gtaatgttgg cgaaacctaa acccgacgca tagagtgcgt
    27301 tatcgttggt ggtccagata ttcagcccga gcaccacaat cgcaggcagc agcagtccct
    27361 gagcaatcat cacatcagag atatccgcca tccccagtgc cgcagcgccc gctgcaccga
    27421 aaataaacat caacgagttg ccgaggaaaa aggccaccat cgccaccagc accgccagtt
    27481 tggcattgcg accaaaccgg acaaagtcag cggtgagcgt acccgcactg ataaatgacc
    27541 ccacaaccag cgccagcgcg acattgaaat ctaacggttg tgcgggaacg accgctttta
    27601 atgcgtccag gccgcccatg ccgttaacag ccagccacac ggaataaccg cccaggcagg
    27661 cgatagccgg aaccgcaatc accgaaagaa ccgtcagcgc cgaaatgcca aaaaagacgg
    27721 tgacggtcat cagtaaaccg gaaacggcaa tcagcaaatt aatatccagc ccggttgcct
    27781 tacccaccgg aatggcaaac atcgccacac cgacgccaaa ccagccaacc tgagttccgc
    27841 ccagtagcag tgaaggcagc catgagcctt taacaccaaa cgagaagcga gcaagaagat
    27901 gagtggtcag gccggttttt gcgccaatgt aaccgagaaa tgaagtgtaa ataccgagga
    27961 gaagattacc gatgagaact gcgaggaaga aatcatgata gctaagaccg gttccgagag
    28021 tgccgccggt ccacatactg gcggaaaaga aggttaatcc cagcatgacg aacgtcaatg
    28081 ccaatacccc tttccgcgcc gactgcggga ctggcccctg gctaaagttg ttatcttgcg
    28141 acacgaaatt cccccttttt gctgtttgaa acccaaaaaa tccgccgcat tctattcatc
    28201 tgaagataaa aagcaatcgt tttcgtgggg aaatatattt tttatat
//
LOCUS       DAJOSY010000055        28243 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJOSY010000055 DAJOSY010000000
VERSION     DAJOSY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.055"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..344
                     /locus_tag="OHN27_002465"
     CDS             <1..344
                     /locus_tag="OHN27_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCQ2286884.1"
                     /translation="MEKISLGQREELKSFAFDLLLELYDNELQYTDELYAETPWADDV
                     KAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSGSSYVMGK
                     AVETEDEDWNF"
     gene            699..1901
                     /gene="proV"
                     /locus_tag="OHN27_002466"
     CDS             699..1901
                     /gene="proV"
                     /locus_tag="OHN27_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCQ2286885.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKAIDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1894..2958
                     /gene="proW"
                     /locus_tag="OHN27_002467"
     CDS             1894..2958
                     /gene="proW"
                     /locus_tag="OHN27_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCQ2286886.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            3015..4007
                     /gene="proX"
                     /locus_tag="OHN27_002468"
     CDS             3015..4007
                     /gene="proX"
                     /locus_tag="OHN27_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCQ2286887.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            4199..5383
                     /locus_tag="OHN27_002469"
     CDS             4199..5383
                     /locus_tag="OHN27_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2286888.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            5507..6244
                     /gene="ygaZ"
                     /locus_tag="OHN27_002470"
     CDS             5507..6244
                     /gene="ygaZ"
                     /locus_tag="OHN27_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCQ2286889.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            6234..6569
                     /gene="ygaH"
                     /locus_tag="OHN27_002471"
     CDS             6234..6569
                     /gene="ygaH"
                     /locus_tag="OHN27_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCQ2286890.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6660..7190
                     /gene="emrR"
                     /locus_tag="OHN27_002472"
     CDS             6660..7190
                     /gene="emrR"
                     /locus_tag="OHN27_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCQ2286891.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            7317..8489
                     /gene="emrA"
                     /locus_tag="OHN27_002473"
     CDS             7317..8489
                     /gene="emrA"
                     /locus_tag="OHN27_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCQ2286892.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            8506..10044
                     /gene="emrB"
                     /locus_tag="OHN27_002474"
     CDS             8506..10044
                     /gene="emrB"
                     /locus_tag="OHN27_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCQ2286893.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(10108..10623)
                     /gene="luxS"
                     /locus_tag="OHN27_002475"
     CDS             complement(10108..10623)
                     /gene="luxS"
                     /locus_tag="OHN27_002475"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCQ2286894.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10773..12329)
                     /gene="gshA"
                     /locus_tag="OHN27_002476"
     CDS             complement(10773..12329)
                     /gene="gshA"
                     /locus_tag="OHN27_002476"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCQ2286895.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMPFW
                     QAKCGDISGADGKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(12402..12830)
                     /locus_tag="OHN27_002477"
     CDS             complement(12402..12830)
                     /locus_tag="OHN27_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2286896.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12827..13393)
                     /gene="yqaB"
                     /locus_tag="OHN27_002478"
     CDS             complement(12827..13393)
                     /gene="yqaB"
                     /locus_tag="OHN27_002478"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCQ2286897.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13674..13750)
                     /locus_tag="OHN27_002479"
     tRNA            complement(13674..13750)
                     /locus_tag="OHN27_002479"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13714..13716),aa:Arg,seq:acg)
     gene            complement(13949..14025)
                     /locus_tag="OHN27_002480"
     tRNA            complement(13949..14025)
                     /locus_tag="OHN27_002480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13989..13991),aa:Arg,seq:acg)
     gene            complement(14088..14164)
                     /locus_tag="OHN27_002481"
     tRNA            complement(14088..14164)
                     /locus_tag="OHN27_002481"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14128..14130),aa:Arg,seq:acg)
     gene            complement(14363..14439)
                     /locus_tag="OHN27_002482"
     tRNA            complement(14363..14439)
                     /locus_tag="OHN27_002482"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14403..14405),aa:Arg,seq:acg)
     gene            complement(14443..14535)
                     /locus_tag="OHN27_002483"
     tRNA            complement(14443..14535)
                     /locus_tag="OHN27_002483"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14499..14501),aa:Ser,seq:gct)
     gene            complement(14851..15036)
                     /gene="csrA"
                     /locus_tag="OHN27_002484"
     CDS             complement(14851..15036)
                     /gene="csrA"
                     /locus_tag="OHN27_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCQ2286898.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(15271..17901)
                     /gene="alaS"
                     /locus_tag="OHN27_002485"
     CDS             complement(15271..17901)
                     /gene="alaS"
                     /locus_tag="OHN27_002485"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCQ2286899.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(18029..18529)
                     /gene="recX"
                     /locus_tag="OHN27_002486"
     CDS             complement(18029..18529)
                     /gene="recX"
                     /locus_tag="OHN27_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCQ2286900.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCGLARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQEI
                     WRNFAD"
     gene            complement(18598..19659)
                     /gene="recA"
                     /locus_tag="OHN27_002487"
     CDS             complement(18598..19659)
                     /gene="recA"
                     /locus_tag="OHN27_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCQ2286901.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19739..20236)
                     /gene="pncC"
                     /locus_tag="OHN27_002488"
     CDS             complement(19739..20236)
                     /gene="pncC"
                     /locus_tag="OHN27_002488"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCQ2286902.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(20381..21466)
                     /gene="mltB"
                     /locus_tag="OHN27_002489"
     CDS             complement(20381..21466)
                     /gene="mltB"
                     /locus_tag="OHN27_002489"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCQ2286903.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21722..22285
                     /gene="srlA"
                     /locus_tag="OHN27_002490"
                     /gene_synonym="gutA"
     CDS             21722..22285
                     /gene="srlA"
                     /locus_tag="OHN27_002490"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCQ2286904.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            22282..23241
                     /gene="srlE"
                     /locus_tag="OHN27_002491"
                     /gene_synonym="gutE"
     CDS             22282..23241
                     /gene="srlE"
                     /locus_tag="OHN27_002491"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCQ2286905.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            23252..23623
                     /gene="srlB"
                     /locus_tag="OHN27_002492"
                     /gene_synonym="gutB"
     CDS             23252..23623
                     /gene="srlB"
                     /locus_tag="OHN27_002492"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCQ2286906.1"
                     /translation="MTVIYQTTITRIGASAIDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23627..24406
                     /gene="srlD"
                     /locus_tag="OHN27_002493"
     CDS             23627..24406
                     /gene="srlD"
                     /locus_tag="OHN27_002493"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCQ2286907.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            24511..24870
                     /gene="gutM"
                     /locus_tag="OHN27_002494"
     CDS             24511..24870
                     /gene="gutM"
                     /locus_tag="OHN27_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCQ2286908.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            24937..25710
                     /gene="srlR"
                     /locus_tag="OHN27_002495"
                     /gene_synonym="gutR"
     CDS             24937..25710
                     /gene="srlR"
                     /locus_tag="OHN27_002495"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCQ2286909.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25703..26668
                     /gene="gutQ"
                     /locus_tag="OHN27_002496"
     CDS             25703..26668
                     /gene="gutQ"
                     /locus_tag="OHN27_002496"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCQ2286910.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26665..28179)
                     /gene="norR"
                     /locus_tag="OHN27_002497"
     CDS             complement(26665..28179)
                     /gene="norR"
                     /locus_tag="OHN27_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCQ2286911.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRCLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
BASE COUNT         6552 a         7609 c         7154 g         6928 t
ORIGIN      
        1 acatggaaaa gatatctctg ggacaacgtg aagagttgaa gagtttcgcc ttcgatttgt
       61 tgctggaact ctacgacaac gagttgcaat acaccgatga gctgtacgcc gaaaccccgt
      121 gggctgacga tgtgaaagcg tttctctgtt acaacgccaa taaggctttg atgaatctgg
      181 gctacgaacc gttatttccc gcagaaatgg cggaagtgaa tccggcaatc ctcgccgcgc
      241 tttcgccgaa tgccgatgaa aatcacgatt tcttttccgg ttcaggctcc tcttatgtga
      301 tggggaaagc ggttgaaaca gaagatgaag actggaattt ctgagggtgt tattttcaaa
      361 aatatcacta cccgcagcag ggaaataatt cccgccaaat agctttttat cacgcaaata
      421 ttctgtagtg atctacactg atactctgtt gcattattcg cctgaaacta caatattcag
      481 gcgttttttc gctatctatg acaaaaaata tcaacttttc tcgatttgct ctcagccctt
      541 atatcacggg aaattccggc gatttgctcg catcaatatt catgccacat ttgccctcag
      601 gggttgcctc agattctcag tatgttaggg tagaaaaaag tgactatttc cattgggtaa
      661 tatatcgaca taaacaaata aaggaatctt tctattgcat ggcaattaaa ttagaaatta
      721 aaaatcttta taaaatattt ggtgagcatc cacagcgagc gttcaaatat atcgaacaag
      781 gactttcgaa agaacaaatt ctggaaaaaa ctgggctatc gcttggcgta aaagacgcca
      841 gtctggccat tgaagaaggc gagatatttg tcatcatggg attatccggc tcgggtaaat
      901 ccacaatggt acgccttctc aatcgcctga ttgaacccac ccgcgggcaa gtactgattg
      961 atggtgtgga tattgccaaa atatccgacg ccgaactccg tgaggtgcgc agaaaaaaga
     1021 ttgcgatggt cttccagtcc tttgccttaa tgccgcatat gaccgtgctg gacaatactg
     1081 cgtttggtat ggaattggcc ggaattaatg ccgaagaacg ccgggaaaaa gccattgatg
     1141 cactgcgtca ggtcgggctg gaaaattatg cccacagcta cccggatgaa ctctctggcg
     1201 ggatgcgtca acgtgtggga ttagcccgcg cgttagcgat taatccggat attttattaa
     1261 tggatgaagc cttctcggcg ctcgatccat taattcgcac cgagatgcag gatgagctgg
     1321 taaaattaca ggcgaaacat cagcgcacca ttgtctttat ttcccacgat ctcgatgaag
     1381 ccatgcgtat tggcgaccga attgccatta tgcaaaatgg tgaagtggta caggtcggca
     1441 caccggatga aattctcaat aatccggcga atgattatgt ccgtaccttc ttccgtggcg
     1501 ttgatattag tcaggtattc agtgcgaaag atattgcccg ccggacaccg aatggcttaa
     1561 ttcgtaaaac ccctggcttc ggcccacgtt cggcactgaa attattgcag gatgaagatc
     1621 gcgaatatgg ctacgttatc gaacgcggta ataagtttgt cggcgcagtc tccatcgatt
     1681 cgcttaaaac cgcgttaacg cagcagcaag gtcttgatgc ggcgctgatt gatgcgccgt
     1741 tagcagtcga tgcacaaacg cctcttagcg agttgctctc tcatgtcgga caggcaccct
     1801 gtgcggtgcc cgtggtcgac gaggaccaac agtatgtcgg catcatttcg aaaggaatgc
     1861 tgctgcgcgc tttagatcgt gagggggtaa ataatggctg atcaaaataa tccgtgggat
     1921 accacgccag cggcggacag tgccgcgcaa tccgcagacg cctggggtac accgacgact
     1981 gcaccgactg acggcggtgg tgctgactgg ctgaccagta cgcctgcgcc aaacgtcgag
     2041 cattttaata ttctcgatcc gttccataaa acgctgatcc cgctcgacag ttgggtcact
     2101 gaagggatcg actgggtcgt tacccatttc cgtcccgtct tccagggcgt gcgcgttccg
     2161 gttgattata tcctcaacgg tttccagcaa ttgctgctgg gtatgcccgc accggtggcg
     2221 attatcgttt tcgctctcat cgcctggcag atttccgggg tcggaatggg tgtggcgacg
     2281 ctggtttcgc tgattgccat cggcgcaatc ggtgcctggt cgcaggcaat ggtgactctg
     2341 gcgctggtgt taaccgccct gctgttctgt atcgtcatcg gtttgccgtt ggggatatgg
     2401 ctggcgagaa gtccgcgagc ggcgaaaatt attcgtccac tgcttgatgc catgcagacc
     2461 acgccagcgt ttgtttatct ggtgccaatc gtcatgctat ttggtatcgg taacgtgccg
     2521 ggcgtggtgg tgacgatcat ctttgctctg ccgccgatta tccgtctgac cattctgggg
     2581 attaaccagg ttccggcgga tctgattgaa gcctcgcgct cattcggtgc cagcccgcgc
     2641 cagatgctgt tcaaagttca gttaccgctg gcgatgccga ccattatggc gggcgttaac
     2701 cagacgctga tgctggccct ttctatggtg gtcatcgcct cgatgattgc cgtcggcggg
     2761 ttgggtcaga tggtacttcg cggtatcggt cgtctggata tggggcttgc caccgttggc
     2821 ggcgtcggga ttgtgatcct cgccattatc ctcgatcgtc tgacgcaggc cgttgggcgc
     2881 gactcacgca gtcgcggcaa ccgtcgctgg tacaccactg gccctgtcgg tctgctgacc
     2941 cgcccattca ttaagtaact ctgcacttgc ccggtgacgc cgggcatatc accctgccaa
     3001 aaaaaggaat aacaatgcga catagcgtac tttttgcgac agcgtttgcc acgcttatct
     3061 ctacacaaac ttttgctgcc gatctgccgg gcaaaggtat tactgttaat ccagttcaaa
     3121 gcaccatcac cgaagaaacc ttccagacgc tgctggtcag ccgtgcgctg gagaaattag
     3181 gctataccgt caacaaaccc agcgaagtag attacaacgt tggctacacc tcgcttgctt
     3241 ccggcgatgc aaccttcacc gccgtgaact ggacgccact gcatgacaac atgtacgaag
     3301 ctgccggtgg cgataagaaa ttttatcgtg aaggggtatt tgttaacggc gcggcacagg
     3361 gttacctgat cgataagaaa accgccgacc agtacaaaat caccaacatc gcacaactga
     3421 aagatccgaa gatcgccaaa ctgttcgata ccaacggcga cggaaaagcg gatttaaccg
     3481 gttgtaaccc tggctggggc tgcgaaggtg cgatcaacca ccagcttgcc gcgtatgaac
     3541 tgacccatac cgtgacgcat aatcagggga actacgcggc gatgatggcc gacaccatca
     3601 gtcgctacaa agagggcaaa ccggtgtttt actacacctg gacgccgtac tgggtgagta
     3661 acgaactgaa gccgggcaaa gatgtcgtct ggttgcaggt gccgttctcc gcactgccgg
     3721 gcgataaaaa cgccgatacc aaactgccga atggtgcgaa ttatggcttc ccggtcagca
     3781 ccatgcatat cgttgccaac aaagcctggg ccgagaaaaa cccggcagca gcgaaactgt
     3841 ttgccattat gcagttgcca gtggcagata ttaacgccca gaacgccatt atgcatgacg
     3901 gcaaagcctc agaaggcgat attcagggcc atgttgatgg ctggatcaaa gcccaccagc
     3961 agcagttcga tggctgggtg aatgaggcgc tggcagcgca gaagtaattt ttattcgggc
     4021 ggataaggcg tttttacctc atccgccgct ctgtacacaa tgcctgatgc gacgctgacg
     4081 cgtcctatca tgcctacaaa ccacatcgca catttcaaca agctatcttt catcccatat
     4141 tcatcaacat ccgctattat tgatttccag cttaatcatc acctgatgaa caaaaataat
     4201 gactaaacct aatcatgagc ttagcccggc gctgatcgtg ctgatgtcta tcgccaccgg
     4261 tctggcggtc gccagtaact attacgctca gccattgctc gacaccatcg cgcgtaactt
     4321 ttccctttcc gccagttcgg caggctttat tgttaccgcc gcgcaattgg gctatgcagc
     4381 aggtctgctg tttcttgttc ccctcggtga tatgtttgaa cgccgccgcc tgattgtctc
     4441 gatgacctta ctggcggcag gcggcatgtt aatcactgcc agcagtcagt cgctggcgat
     4501 gatgatcctc ggtacggcat taaccggttt attctctgtc gtggcacaaa ttctggttcc
     4561 gctggcagcg acgctggctt caccggacaa acgcggcaaa gtggttggca ctattatgag
     4621 cggtctgctg ttggggatct tgctggcacg gacagttgcc ggattgctgg cgaatctcgg
     4681 cggctggcgc accgtctttt gggttgcttc ggtgttaatg gcactgatgg cgctggcatt
     4741 atggcgtggt ctgccacaaa tgaaatcaga aacccacctc aactacccac agttgttggg
     4801 ttccgttttc agtatgttta tcagcgataa aattctgcgt acccgcgcgt tgctgggctg
     4861 cctgaccttt gccaatttca gcattctctg gacctcaatg gcctttttgc ttgccgctcc
     4921 accttttaac tacagcgatg gtgtcattgg tctgtttgga cttgcgggag ctgccggagc
     4981 gttgggcgct cgtccggcgg gcggttttgc cgataagggc aaatcgcacc acaccacaac
     5041 tttcggtctg ctgctgctat tactttcatg gctggcgatc tggtttggtc acacttccgt
     5101 actggcgttg attatcggaa tcctggtgct ggatctcacc gtgcagggcg tgcatatcac
     5161 taaccagacg gtaatttatc ggatacatcc tgatgcgcgc aatcgcctga ccgcaggtta
     5221 catgactagc tactttattg gcggtgccgc cggttcgcta atttcagcct cagcctggca
     5281 acatggcggt tgggctggcg tttgtctggc tggcgcgaca attgccctgg ttaacttact
     5341 ggtctggtgg cgaggttttc atcgtcagga agccgcaaat taagcaatca gcaaccgttt
     5401 tcgtggcttt acacttataa gggtgttaag aagcccatca gtctgataag gttaagatat
     5461 tcattcagtc tatttataat attaacaatc gttaagcgta cactctatgg aaagccctac
     5521 tccacagcct gctcctggtt cggcgacctt catggaagga tgcaaagaca gtttaccgat
     5581 tgttattagt tatattccgg tggcctttgc gttcggtctg aatgcgaccc gtctgggatt
     5641 ctctcctctc gaaagcgttt ttttctcctg catcatttat gcaggcgcga gccagttcgt
     5701 cattaccgcg atgctggcag ccgggagtag tttgtgggtt gctgcactga ccgtcatggc
     5761 aatggatgtt cgccatgtgt tgtatggccc gtcactgcgt agccgtatta ttcagcgtct
     5821 gcaaaaatcg aaaaccgccc tgtgggcatt tggcctgacg gatgaggttt ttgccgccgc
     5881 aaccgcaaaa ctggtacgca ataatcgccg ctggagcgag aactggatga tcggcattgc
     5941 cttcagttca tggtcatcgt gggtatttgg tacggtaata ggggcattct ccggcagcgg
     6001 cttgctgcaa ggttatcccg ccgttgaagc tgcattaggt tttatgcttc cggcactctt
     6061 tatgagtttc ctgctcgcct ctttccagcg caaacaatct ctttgcgtta ccgcagcgtt
     6121 agttggtgcc cttgcaggcg taacgctatt ttctattccc gtcgccattc tggcaggcat
     6181 tgtctgtggc tgcctcactg cgttaatcca ggcattctgg caaggagcgc ccgatgagct
     6241 atgaggttct gctgcttggg ttactggttg gcgtggcgaa ttattgcttc cgctatttgc
     6301 cgctgcgcct gcgtgtgggt aatgcccgcc caaccaaacg tggcgcggta ggtattttgc
     6361 tcgacaccat tggcatcgcc tcgatatgcg ctctgctggt tgtctctacc gcaccagaag
     6421 tgatgcacga tacacgccgt ttcgtgccca cgctggtcgg cttcgcggta ctgggtgcca
     6481 gtttctataa aacacgcagc attatcatcc caacactgct tagtgcgctg gcctatgggc
     6541 tcgcctggaa agtgatggcg attatataac ccacaagaat catttttcta aaacaataca
     6601 tttactttat ttgtcactgt cgttactata tcggctgaaa ttaatgaggt catacccaaa
     6661 tggatagttc gtttacgccc attgaacaaa tgctaaaatt tcgcgccagc cgccacgaag
     6721 attttcctta tcaggagatc cttctgactc gtctttgcat gcacatgcaa agcaagctgc
     6781 tggagaaccg caataaaatg ctgaaggctc aggggattaa cgagacgttg tttatggcgt
     6841 tgattacgct ggagtctcag gaaaaccaca gtattcagcc ttctgaatta agttgtgctc
     6901 ttggatcatc ccgtaccaac gcgacgcgta ttgccgatga actggaaaaa cgcggttgga
     6961 tcgaacgtcg tgaaagcgat aacgatcgcc gctgcctgca tctgcaatta acggaaaaag
     7021 gtcacgagtt tttgcgcgag gttttaccac cgcagcataa ctgcctgcat caactctggt
     7081 ccgcgctcag cacaacagaa aaagatcagc tcgagcaaat cacccgcaaa ttgctctccc
     7141 gtctcgacca gatggaacaa gacggtgtgg ttctcgaagc gatgagctaa cgcgtcatct
     7201 cgctcaaaaa tccagattta taaaagaaaa aatgaccggc cagcatcgca acatgctggc
     7261 ctttttggca agcaggtcgg ctcagccgat gagttaagaa gatcgtggag aacaatatga
     7321 gcgcaaatgc ggagactcaa accccgcagc aaccggtaaa gaagagcggc aaacgtaagc
     7381 gtctgctcct ccttctcacc ttgctcttta taattattgc cgtagcgata gggatttatt
     7441 ggtttttggt actgcgtcat ttcgaagaaa ccgatgacgc atacgtggca gggaatcaga
     7501 ttcaaattat gtctcaggtg tctggcagcg tgacgaaagt ctgggccgat aacaccgatt
     7561 ttgtaaaaga aggcgatgtt ctggtcactc tcgacccgac agatgctcgc caggcgtttg
     7621 aaaaagccaa aactgcactg gcttccagcg ttcgccaaac ccaccagctg atgattaaca
     7681 gcaaacagtt gcaggcgaat attgaggtgc aaaaaatcgc cctcgcgaaa gcacaaagcg
     7741 actacaaccg ccgtgtgccg ctgggcaatg ccaacctgat tggtcgcgaa gagctgcaac
     7801 acgcccgcga cgctgtcacc agtgcccagg cgcaactgga cgtcgctatt caacaataca
     7861 atgccaatca ggcgatgatt ctggggacta aactggaaga tcagcctgcc gtgcaacagg
     7921 ctgccaccga agtacgtaac gcctggctgg cgctggagcg tacccgtatt gtcagtccga
     7981 tgaccggtta tgtctcccgc cgcgcggtac agcctggggc gcaaattagc ccaacgacgc
     8041 cgctgatggc ggtcgttcca gccaccaata tgtgggtgga tgccaacttt aaagagacgc
     8101 agattgccaa tatgcgtatc ggtcagccgg tcaccatcac caccgatatt tacggcgatg
     8161 atgtgaaata caccggtaaa gtggttggtc tggatatggg cacaggtagc gcgttctcac
     8221 tgcttccggc gcaaaatgcg accggtaact ggatcaaagt cgttcagcgt ctacctgtgc
     8281 gtatcgaact ggaccagaaa cagctggagc aatacccgct gcgcattggt ttgtccacgc
     8341 tggtgagcgt caataccact aaccgtgacg gtcaggtact ggcaaataaa gtacgttcaa
     8401 caccggtcgc ggtaagtacc gcgcgtgaaa tcagcctggc acctgtcaat aaactgatcg
     8461 acgatatcgt aaaagctaac gctggctaat ccagaggtgc gtgtgatgca acagcaaaaa
     8521 ccgctggaag gcgcgcaact ggtcattatg acgattgcgc tgtcactggc gacattcatg
     8581 caggtgctgg actccaccat tgctaacgtg gcgatcccca ctatcgccgg gaatctgggc
     8641 tcatcgctca gccagggaac gtgggtaatc acttctttcg gggtggcgaa tgccatctcg
     8701 atcccgctta ccggctggct ggcaaagcgc gtcggggaag tgaaactgtt cctttggtcc
     8761 accatcgcct ttgctattgc gtcgtgggcg tgtggtgtct ccagcagcct gaatatgctg
     8821 atcttcttcc gcgtgattca ggggattgtc gccgggccgt tgatcccgct ttcgcaaagt
     8881 ctattgctga ataactaccc gccagccaaa cgctcgatcg cgctggcgtt gtggtcgatg
     8941 acggtgattg tcgcgccaat ttgcggcccg atcctcggcg gttatatcag cgataattac
     9001 cactggggct ggatattctt catcaacgtg ccgattggcg tggcggtggt gttgatgaca
     9061 ctgcaaactc tgcgcggacg tgaaacccgc accgaacggc ggcggattga tgccgtgggg
     9121 ctggcactgc tggttattgg tatcggcagc ctgcagatta tgctcgaccg cggtaaagag
     9181 ctggactggt tttcatcaca ggaaattatc atccttaccg tggtggcggt ggtggctatc
     9241 tgcttcctga ttgtctggga gctgaccgac gataacccga tagtcgatct gtcgttgttt
     9301 aagtcgcgca acttcaccat cggctgcttg tgtatcagcc tcgcgtatat gctctacttc
     9361 ggcgctattg ttctgctgcc gcagttgttg caggaggtct acggttacac ggcaacgtgg
     9421 gcaggtctgg cctctgcgcc ggtagggatt attccggtga tcctgtcgcc gattatcggc
     9481 cgcttcgcgc ataaactgga tatgcggcgg ctggtaacct tcagctttat tatgtatgcc
     9541 gtctgcttct actggcgtgc ctataccttt gaaccaggta tggattttgg cgcgtcggcc
     9601 tggccgcagt ttatccaggg gtttgcggtg gcctgcttct ttatgccgct gaccaccatt
     9661 acgctgtctg gtttgccacc ggaacgactg gcggcggcat cgagcctctc taactttacg
     9721 cgaacgctgg cggggtctat cggcacgtcg ataaccacga ccatgtggac caaccgcgag
     9781 tcgatgcacc atgcgcagtt gactgagtcg gtaaacccgt ttaacccaaa cgcccaggcg
     9841 atgtacagtc aactggaagg gcttgggatg acgcaacagc aggcctccgg ctggattgcc
     9901 cagcagatca ccaaccaggg gctgattatt tccgccaatg agatcttctg gatgtcagct
     9961 gggatattcc tcgtcctgct ggggctggtg tggtttgcta aaccgccatt tggcgcaggt
    10021 ggcggcggag gcggtgcgca ctaagtacaa ctaagccagt tcatttgaac tggctttttt
    10081 caattaattg tgaagatagt ttactgacta aatgtgcagt tcctgcaact tctctttcgg
    10141 cagcgccagt tcttcgttgc tgttgatgcg cacgtcacgt tccagaatgc tacgcgcaat
    10201 atcctgtgct tcctgcaacg agtgcatctg gtaagtgcca cactggtaga cgttcagttc
    10261 cgggatctga ttctgatcct gcactttcag cacgtcttcc attgccgctt tccaggcatc
    10321 agcaacacgc tgctcatctg gcgtaccaat cagactcata taaaaaccgg tgcggcagcc
    10381 cattggcgag atatcgataa tctctacacc attaccgtta agatggttac gcataaaacc
    10441 agcaaacagg tgctccaggg tatggatccc tctttctggc atcacttctt tgttcggcac
    10501 gcagaagcgc agatcgaaca cggtgattgc gtcgccatgc ggggtgttca ttgttttcgc
    10561 cacccgaact gcaggcgctt ccatccgggt atgatcgact gtgaagctat ctaacaacgg
    10621 catttagcca cctccggtaa tttttttaaa aattttctga actctttctt cccaggcgag
    10681 tctgagtata tgaaagacgc gcatttgtta tcatcatccc tgaattcaga gatgaaattt
    10741 tggccactca cgagtggcct ttttcttttc tgtcaggcgt gtttttccag ccacaccgca
    10801 aacggttcgg tatctgcggc ttccatttcc tgctgacggc gttcagatgc ctcgcgctcg
    10861 gctacaaaat cctcttcgcg cagaatttcc agcggctctt cacgcagcag attacggtac
    10921 gcttcagcaa acgctttgcc tgttccgcca ataccagtat caatcataga ccttaagata
    10981 cgggcagaga aagtcagatc gggattatcg aagcaggcaa ccagttcatc acacactttc
    11041 tgatacgctt cgccgccgtt aatgctatcc agcgtttgcg cgacgcgttt cagatcgcgg
    11101 aacagatctt tacccacctg cggtaacggg aactgtgcgg tttcgcagcc gatacccagc
    11161 gtcagacccg gtttgcgacc ttcgaggatc acccggttcc agttaacgcg tgtacaggca
    11221 agttcgctac tgctcatttc cggtgcatca gccagcgcac accagaccat aaacaggtcg
    11281 aggaatcgca cctgctgttc atctacacca atcggcgaga acgggttgat gtccagcgaa
    11341 cgcacttcaa tatattcaat accgccacgt aacagcgcat cagaaggcga ctcgccgctg
    11401 cgggtaacgc gttttggacg aatcggcgcg tacagttcgt tttcaatctg cagcacgttg
    11461 ctgttgattt gcagcctctt accgtctttc tcaataccaa tcttcgcgta ctcttccgat
    11521 ggcgttttga ttgcctgttt aaggcccgct acgtactcgt aaagatcgtt gaaggtaata
    11581 ccaagattgc tttgcgattt attggtatag ccgagatcgc tcaaacgaag agaggtcgca
    11641 tacggcaggt aatacatacc gcactcggtt ttctcaaacg gcagcgatgt tggttttcct
    11701 tgcaggaaag aagaacaaat cgccggagac gcgccaaaca gataaggaat gacccaaccg
    11761 aaacgatagt aattgcggat aacgcggaaa tagcccgcag aaattttctc tttgccatca
    11821 gcgcccgaga tatcaccaca cttcgcttgc cagaatggca ttggcaaaga gaaattgtag
    11881 tgcacgccgg aaatggtttg catcagcgcg ccatagcgat ttttcagccc ttcacgatac
    11941 agcgttttaa agcgtccggt gttagaagtg ccgtactgtg ccagttcgat gtcctgacct
    12001 tctgcgatgt agcatggcat acttaacggc cacatccgct catcgcccat attgcgcgcc
    12061 gtataacgat gcagatcgcg cataaaggtc agcatatgtt caatatcacc atccactggt
    12121 gtaatgaatt ccagcaatgc ttccgcaaaa tcggtagtaa tccatttgtg cgtcagtgcg
    12181 gaacctaatg cttcaggatg acctgttgtt gccagtgtgc catcagcatt aacacgcaaa
    12241 gtttcgcgct ccagcccacg ctgtatcccc tttaacgcct gaggatgttt ttccagccag
    12301 gccagcgcct gtgatacgtc cgggatcaaa ttgacctccc gcctgtcaaa atcgttttaa
    12361 ttagcataac tgtaatggtg accatatgtg caggcctaca attagtgcca ccacatcatg
    12421 ccctgaacgg tcgctgctgc aactgcaaca tagcgtaacg ctttaccaag gcacaaaaaa
    12481 aagattaccg gtccccacga gatgcgcatc catcccgcta acagacacag taaatcgcca
    12541 accacgggca tccagcttaa taatagcgtg actgcaccat agcgtttcag ccagccggta
    12601 gctttctctt gccagcgcga tgttttacgc aatggaaaga aacgcccaag gataacgtta
    12661 gttaaccctc caaggctatt acccattgtt gctgttaaaa ctaaaaccca gggatgactg
    12721 atcccggaaa gcaacattgc caccagcacg acttcggagt tgccgggtaa tagtgtagcg
    12781 ctgagaaaac tactggcaaa caacgagaaa agcgataacg cttcactcac agcaagcgaa
    12841 catccacggc gtccatgcct gctgcacggg ccgcctgaat accgaaatcg gcatcttcaa
    12901 agaccacaca ctgcgtcggt tgcacgccca tacgctgcgc gcacaacaaa aatgtgtctg
    12961 gcgcgggttt atggtgtttg acgtgatcgg cagcgacgac ggcgtcaaaa taatggcgta
    13021 atcccaggtg cgccagcaat gcctcagcga tggcgctttc actccccgtt cctacagcca
    13081 ttgggcgacg accatgccaa cttttcacca catcaacaag aggaagcggt tcgacgctat
    13141 ccagcagcat acttcttact gcttctgttt tttcacgcgc taacgcatgc gggtcgagat
    13201 cggcctgatt cagctcaata attgcctgag caatacgcca ggtgggcgat ccattaagcg
    13261 caatcatcgc ctgaatatcg tactgaagac cgtagtgccc taatacttcg cgccacgctt
    13321 tacggtgcgt aggctccgta tccaggattg tgccatccat atcaaaaatt aaacctgcat
    13381 aacgctcgta catggtcttc tcgcaagtcg aaaaatatga cgctacttta gcgtaattgc
    13441 ttgattttgt cgctgatggc gaaggagaag aaaattcagg aaatggataa aggagttatt
    13501 tagaaatgag atatttttga aggaaatttt ttggagaaga tggtgcatcc gggaggatga
    13561 ctcactgacg ttcgcccttc gggccgttgc tggcgcaacg ttatccttcc tggtgcctgc
    13621 ggttaatacc gctatctctt cgataccttc attgctgaag gttacggaga atatggtgca
    13681 tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag
    13741 ctacggatgc atcgggaaaa cttattctac tgctgatatt gataccgcta ctaaagccat
    13801 atcaattaag agatggtgca tccgggagga ttactcgcct tgcggctcgc ccttcgggcc
    13861 gttgctaaag caacgttatc atccctagtg cctgcggtta attccgctac ctcttcgata
    13921 ccttcattgc tgaagatttc ggagaatatg gtgcatccgg gaggattcga acctccgacc
    13981 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcatcgg gaaatttatt
    14041 ttactgctga tattgatacc gctactaaag ccatatcaag taagagatgg tgcatccggg
    14101 aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc tgagctacgg
    14161 atgcatcggg aaacttactt tactgcagat tttttgatac cgctactaaa gccgtatcaa
    14221 gtaagagatg gtgcatccgg gaggattact cgccttgcgg ctcgcccttc gggccgttgc
    14281 taaagcaacg ttatcctccc tggtgcctgc ggttattacc gccacctctt ccataccttc
    14341 atcgctgaaa gtacgtagaa tatggtgcat ccgggaggat tcgaacctcc gaccgctcgg
    14401 ttcgtagccg agtactctat ccagctgagc tacggatgca aatggcggtg aggcggggat
    14461 tcgaaccccg gatgcagctt ttgaccgcat actcccttag caggggagcg ccttcagcct
    14521 ctcggccacc tcaccacacg cctcttacga gtgcttcgaa gaacttgttt atcgctcatc
    14581 gtcgctgcgt ggcgcacata ttactttctg agacttataa gtcaaacaat ttttcctaca
    14641 cttttatcgt ttgcacactt cacgttcaat tagtctgtaa aaacgtcaaa aagagtgttt
    14701 tatcaacaga agaatggagg tctgacagat agtagtaatg caaaaaaatg gagacttaag
    14761 ttgaatgaac gggagtaaag cgaaaagact atagagtgaa ggagaaattt tgagggtgcg
    14821 tctcaccgat aaagatgaga cgcggaaaga ttagtaactg gactgctggg atttttcagc
    14881 ctggatacgc tggtagatct cttcacggtg aacagaaact tccttcgggg catttacgcc
    14941 aatacgtacc tggttgccct ttacccctaa aactgtcacg gtgacctcat ccccaatcat
    15001 gagggtctca ccaactcgac gagtcagaat cagcattctt tgctccttga aagattaaaa
    15061 gagtcgggtc tctctgtatc ccggcattat ccatcatata acgccaaaaa gtaagcaatg
    15121 acaaacacat tacatctaag cagtcatggc attacattct gttaaaccta agtttagccg
    15181 atatacacaa cttcaacctg actttatcgt tgtcgatagc gttaatgcga atgccgtgaa
    15241 gcgagtccac ggcattgcct gacgcttata ttattgcaat ttcgcgctga cccagccttt
    15301 cacactggct aacgctgcag gtaaggccgc agcatccgta ccaccggctt gcgccatgtc
    15361 aggacgtcca ccacccttgc cgcccacctg ctgagcgacc ataccaatca gttcccctgc
    15421 tttcacacga tctgtgacgt ccttagatac gcctgcaatc agagaaacct taccttcggc
    15481 taccgttgcc agcacgataa ttgtcgaccc cagctgattt tttaaatcgt caaccatggt
    15541 acgcaacatt ttcggctcaa caccgctaag ctcgctaacc aacagcttaa caccattaac
    15601 atcaattgcc ttactggaaa gatttgcgct ctcctgtgcg gcagcttgtt ctttaagctg
    15661 ttgtaactct ttttccagct gacgcgtacg ttccagtact gagcgcactt tatcagccag
    15721 attattgcta tcgcctttca gcagatgtgc gacttcgctt aagcgatcgc tgtctgcatg
    15781 aacggtggcg atagcgcctt ctccggttac cgcttcgata cgacgaacgc ctgcagcagt
    15841 acccgattca gagatgatgc ggaacagacc aatatcacca gtgcggctgg cgtgagtacc
    15901 gccacacagc tcggtggaga aatcgcccat gctcagcacg cgtacgcgct catcatactt
    15961 ctcgccgaac agcgccatcg cacctttcgc tttcgccgct tcgagatcca tgatgttggt
    16021 ttcgatcggc aaattgcgac gaatctgtgc gttcaccagg tcttcgaccg cacgaatctc
    16081 ttctggtttc atcgcttcgt tgtgtgagaa gtcgaagcgc agcaccttgt cgttaaccag
    16141 tgaacctttc tgcgatacat gagtacccag aacctggcgc agcgcagcgt gcatcaggtg
    16201 cgttgcggag tgattcagac gaatacgggc acgacgagcc tcatcaacat cagcctgcac
    16261 cgcgtcgccc actttcagag aacccgcagc aagtttaccg atgtgaccaa tcgcctggcc
    16321 gtatttctgc gtatcttcca ccgcaaagga gaagttagcg cctttcagtt cgcctttatc
    16381 gccaacctga ccgccggatt ccgcatagaa tggcgtttga tccagcacga ccacagcttc
    16441 ctggcctgca ttgatggcat caaccgcttt accatcaaca aacagcgcgg tcactttgcc
    16501 gttcagttcc agatggtcat agcctttaaa ttcagatgca ctatcaacac ggatcattgc
    16561 gttgtaatcg gcaccaaagc cgctggcttc gcgcgcccga cgacgctgct cttccattgc
    16621 tgcttcaaaa ccagcttcgt caactttgat gttgcgctca cgacaaacgt cagccgtcag
    16681 gtcaaccggg aagccatagg tgtcgtacag acggaaagca gtttcaccat ccagcgtatc
    16741 accagaaagt tttgccagct cttcatccag caacgccaga ccgcgctcca gagtacgggc
    16801 aaactgctct tcttcagtct tcagcacctg ctcaacctgc gcctgctggc gtttcaggtc
    16861 ttcacccgca gagcccataa cgtcgatcag cggaccaacc agtttgtaga agaaggtttc
    16921 tttcgcgcca agcatattac cgtgacgcac tgcgcgacga atgatacgac gcagtacata
    16981 accacggttt tcgttggacg gcattacgcc atccgcgatc aggaacgcac aagaacgaat
    17041 gtggtcagcg attacgcgca gcgatttatt gctcagatcg gtcgcgccag tgactttcgc
    17101 taccgcctgg atcaacgtgc ggaacaggtc gatgtcatag ttagagttaa cgtgttgcag
    17161 caccgcagca atacgctcca gacccatacc ggtatctaca gacggcttcg gcagcggttc
    17221 catcgtgcca tcggcctggc ggttgaactg catgaagacg atgttccaga tctcaatgta
    17281 gcggtcgccg tcttcttccg gacttcccgg agggccaccc caaatgtggt cgccgtgatc
    17341 gtagaagatt tcggtgcacg ggccgcacgg accagtgtca cccatctgcc agaagttgtc
    17401 agatgcgtat ggcgcacctt tgttatcgcc gatgcgaata atacgttcgc gcgggatccc
    17461 gacttctttt tcccagattt cgtaggcttc gtcgtcgctt tcatagacgg tgacccacag
    17521 acgctctttc ggcagggcaa accatttttc gctggtcagc agttcccatg caaactgaat
    17581 ggcatcgtgt ttgaaatagt cgccgaagct gaagttgccc agcatttcga agaaggtatg
    17641 gtgacgcgcg gtgtaaccga cgttttccag gtcgttgtgt ttaccacccg cacgcacgca
    17701 gcgttgggaa gtggtagcgc gggaataatt acgcttgtcg agcccaagga acacatcctt
    17761 gaactggttc atcccggcgt tggtaaacaa caaagttggg tcgttatggg gtaccaggga
    17821 gctgctggca actacctgat gtcccttact atggaaaaag tcgagaaacg cctgacggat
    17881 ctcagcggtg ctcttgctca taattatcct gaaatcaagc taacgaaata tcgccaccag
    17941 ctccagcgtg tcttaaccgc cgggctggta actgaaaagt gggaataaga taagttttct
    18001 tgactgggaa gtaaaatacc gtatgcgttc agtcggcaaa atttcgccaa atctcctgga
    18061 tatcttccat cagatagcca cgatagagca gaaaacgctg gatcttaact ttttctgaaa
    18121 agacagttgg caaaggttcg ccatattttc gcgtcgcctg atcgcgcgcc agtccgcacc
    18181 agtcgatgtc acattcacgc atcgcttttt ctgtcgcttc gcgggaaata cctttctgat
    18241 tcagttcctg gcgaatacgc gcaggtccat aacctttgcg gctacggctg gcgataaagc
    18301 gcgcaacaaa tcggctgtca tcgagatagc catgttcatg gcaccaggca ataacgcgct
    18361 cgtaatcttc tgccgtagca tcaatctctt ctgggccatt tttgcccata atcggtgccg
    18421 cgagtttacg tcgcagttct tgctcactgt gatcgcgcac cgccagaatg cgtaccgcac
    18481 gatccaacag gcgagcatat gccgggcgac gggatgttga ttctgtcatg gcatatcctt
    18541 acaacttaaa aaagcaaaag ggccgcagat gcgacccttg tgtatcaaac aagacgatta
    18601 aaaatcttcg ttagtttctg ctacgccttc gctatcatct acagagaaat ccggcgttga
    18661 gttcgggttg ctcagcagca actcacgtac tttcttctcg atctctttcg cggtttccgg
    18721 gttatctttc agccaggcag tcgcattcgc tttaccctga ccgatcttct cacctttgta
    18781 gctgtaccac gcgcctgctt tctcgatcag cttctctttt acgcccaggt caaccagttc
    18841 gccgtagaag ttgatacctt cgccgtagag gatctggaat tcagcctgtt taaacggcgc
    18901 agcgattttg ttcttcacca ctttcacgcg ggtttcgcta cccaccacgt tttcgccctc
    18961 tttcaccgcg ccgatacgac ggatgtcgag acgaacagag gcgtagaatt tcagcgcgtt
    19021 accaccggta gtggtttccg ggttaccgaa catcacacca attttcatac ggatctggtt
    19081 gatgaagatc agcagcgtgt tggactgctt caggttaccc gccagcttac gcatcgcctg
    19141 gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg atttcgcctt cgatttccgc
    19201 tttcggcgtc agtgccgcca cggagtcaac gacgataacg tctactgcgc cagaacgcgc
    19261 cagggcgtca cagatttcca gtgcctgctc gccggtgtcc ggctgggagc acagcaggtt
    19321 gtcgatatcg acgcccagtt tacgtgcgta gattgggtcc agcgcgtgtt cagcatcgat
    19381 aaacgcacag gttttacctt cacgctgcgc tgcggcgatc acctgcagcg tcagcgtggt
    19441 tttaccggaa gattccggtc cgtagatttc gacgatacgg cccatcggca gaccacctgc
    19501 cccaagcgcg atatccagtg aaagcgaacc ggtagagatg gtttccacat ccatggaacg
    19561 gtcttcaccc aggcgcatga tggagccttt accaaattgt ttctcaatct ggcccagtgc
    19621 tgccgccaac gctttctgtt tgttttcgtc gatagccatt tttactcctg tcatgccggg
    19681 taataccgga tagtcaatat gttctgttga agcaattata ctgtatgctc atacagtatc
    19741 aagtgttttg tagaaattgt tgccacaagg tctgcaatgc atacgcagta gcctgacgac
    19801 gcaccgcatc acggtcgccg ctgaagcatt cccgccgggt aatgccttca ccgcgggcag
    19861 tggcaaaagc aaaccagacg gtgccgacag gcttctcttc actgccgcca tccggcccgg
    19921 cgataccact aatagacacg gcataatcag cacgagccgc tttcagtgcg cctatcgcca
    19981 tttccaccac gacgggttca ctcaccgcgc catgctgcgc cagcgtctct tcgcgtacgc
    20041 cgatcatctg cgctttggct tcgttactgt aggtgacaaa tccgcgttca aaccaggcgg
    20101 agctaccggc aatatcggta atcactttcg ctacccaacc accggtacaa gactcggcag
    20161 ttgttacggt tgcgccacgg gctttcagcg cctgcccaac ctgttcactt aactgcatca
    20221 gttcactgtc agtcatcaca acctctgtca gtcaaaaatt tatgccggac aagatagcac
    20281 tttcgtcagg aagatggtgt tggcgttgca attttacgag gaggattcag aaaaaagctg
    20341 attagccaga gggaagctca cggccccctc ttgtaaatag ctactgtact cgcgccagcg
    20401 ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg ggtgctgtgg ttgtaacggg
    20461 tgatggtgta gaagttcggc agaccgtacc agtactggta gccggtgcca acatccagac
    20521 gcagcaggct ggcttgttga tggttgccca gcggctgctg tggcgttaaa cctgcggcgg
    20581 caagctgcga aatgctgtac ttagttttga agccatttgg caagcctgga gcctgaccgt
    20641 ttgccattac cgcgacctga tcgcctttca cccagccgtg cgctttgaaa tagttcgcca
    20701 cgctaccgat cgcatcaacc ggatcccaca ggttgatatg cccgtcgccg ctgaaatcta
    20761 ccgcatattg tttgtaagac gacggcataa actgtccgta gcccatcgcc ccggcaaagg
    20821 aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc catcaacagg aaggtttcca
    20881 gctcgccaga gaaatactcc gcgcggcgtg ggtagttaaa tgacagcgtt gccagcgcat
    20941 cgaggatgcg agttttcccc atcacgcgcc cccagcgggt ttcaacgccg ataatcccga
    21001 cgataatttc cggcggtacg ccatacacct gccacgcgcg attcaacgca tcttcatact
    21061 gattccagaa aaccacaccg ttctgcacgt tgtccggcgt aataaatttt ttgcgataac
    21121 gcagccatgc gccgttcgga cctgatggcg gtttcaccga tgtggttggt gcctggttat
    21181 ccatcagccg cagtaccgaa tccagacgtt tcgcctggga gagaatttcc tgcaactgct
    21241 gacgatcgaa accgtgtttg ttcaccattt tgtcgatgaa ctgctgggca ttcgggttat
    21301 tagcgaaatc gccgcccatc tgcatcacat tgtgctgcgg ctcaagcagg aagccgccag
    21361 acggcgttcc ggtggtcgta tcagtctcag taggttttgg cttgctgcta caggcggcaa
    21421 gcaacacaaa aaggggaagc aatgttacat aacgacgctt gaacatgagg ggtccattta
    21481 acagattcaa ccaggggcaa gtatggtaaa gcatcacgcc ccgcacaagg aagcggtagt
    21541 cactgcccga tacggacttt acataactca actcattccc ctcgctatcc ttttattcaa
    21601 actttcaaat taaaatattt atctttcatt ttgcgatcaa aataacactt ttaaatcttt
    21661 caatctgatt agattaggtt gccgtttggt aataaaacaa taaatcctga aggagagaac
    21721 aatgatagaa accattactc atggtgcaga gtggtttatc gggctgttcc aaaagggcgg
    21781 agaggtgttt accgggatgg tgaccggcat tcttccgctg ttgattagcc tgctggttat
    21841 catgaacgca ctgattaatt ttatcggtca gcatcgtatt gaacgttttg ctcaacgttg
    21901 cgccggtaac cctgtttccc gttacctact gttaccgtgc attggcacgt ttgtcttttg
    21961 caatccgatg accctaagcc tgggtcgctt tatgccggaa aagtacaaac ccagctacta
    22021 cgcggcggcc tcttatagct gccactcaat gaatggcctc ttcccccata tcaaccctgg
    22081 cgaactgttt gtttatcttg gcattgccag cggtctgaca acgctgaacc tgccacttgg
    22141 cccactggcg gtgagttatc tgctggttgg tctggtcacc aatttcttcc gcggctgggt
    22201 gaccgatctg accaccgcca tttttgagaa aaagatgggc attcaacttg aacaaaaagt
    22261 tcacctggca ggagcaacat catgacgcat attcggatcg aaaaaggaac gggtggctgg
    22321 ggcggcccgc ttgagctgaa agccacgccg ggaaaaaaaa tcgtctatat caccgccggt
    22381 acccggcctg cgattgttga caaactggca cagcttactg gctggcaggc tattgacgga
    22441 tttaaagaag gtgaacccgc ggaggcggaa attggtgtcg cggtaatcga ctgtggcggc
    22501 acattacgct gcggcatcta tccgaaacga cgtattccca ccattaatat ccactcgacg
    22561 ggcaagtccg gtccgctggc gcagtacatt gtggaagata tttatgtctc tggcgtaaaa
    22621 gaagaaaaca tcactgtagt aggtgatgcg acaccacaac cctcttccgt gggccgtgac
    22681 tatgacacca gtaagaaaat caccgaacaa agcgatggtt tactggcgaa ggtgggaatg
    22741 ggcatggggt ccgccgttgc ggtgctgttt caatctggtc gtgacaccat cgacactgta
    22801 ttaaaaacca ttctgccgtt tatggcattc gtctcggcgc tcattggcat cattatggct
    22861 tctggccttg gtgactggat tgcccacggt cttgctccgc tggcgagcca tccactgggt
    22921 ctggtcatgc tggcgctcat ctgttccttc ccactgcttt cacctttcct cggcccaggc
    22981 gcagttatcg cacaggttat cggcgtattg attggcgtgc agattggtct cggcaatatt
    23041 ccgccgcatc tggctttacc ggcactgttt gccatcaacg cgcaggcggc ctgcgacttc
    23101 atcccggtcg gtttgtcgct ggcggaagcc cgtcaggaca cggttcgcgt cggtgtccct
    23161 tctgtactgg tgagccgctt tttaaccggc gcgccgactg tactgatcgc ctggtttgtc
    23221 tccggtttta tctatcaata gaggctgaaa catgaccgtt atttatcaga ccaccatcac
    23281 ccgtatcggc gcgagtgcca ttgacgccct cagtgaccag atgctcatca cctttcgtga
    23341 aggcgcgcct gcggacctcg aagagtattg cttcattcat tgccacggcg agttgaaagg
    23401 tgcactccat cccggtttgc aattttcact cgggcagcat cgctatccgg tgaccgctgt
    23461 tggcagcgtg gcggaagaca accttcgcga actgggtcat gtcaccctgc gcttcgatgg
    23521 tttaaacgaa gcggaatttc cgggcactgt ccatgtggca ggccctgtcc ccgacgatat
    23581 cgcgccggga tcggttttga agtttgaatc tgttaaggag taaaaaatga atcaggttgc
    23641 cgttgtcatc ggtggtgggc aaaccttagg cgcgttcctg tgccacggtc tggctgccga
    23701 ggggtatcgc gtcgcggttg tcgatattca gagcgacaaa gccgcaaatg tggcacaaga
    23761 aattaacgcc gaatatggtg aaagtatggc gtacggtttt ggtgctgacg ccactagcga
    23821 gcaaagcgtt ctggcgctct ctcgtggggt agatgaaatc tttggtcgcg tggatttgct
    23881 ggtctacagc gccggaatag caaaagcagc ctttatcagc gacttccagc tcggcgattt
    23941 tgaccgttcg ctacaggtga atctggtggg ttatttcctg tgtgcgcgtg aattttcgcg
    24001 tttgatgatc cgcgacggga ttcaggggcg cattattcag atcaactcga aatccggcaa
    24061 agtgggcagc aaacacaact ctggctacag cgcagcgaaa tttggtggcg tcgggctgac
    24121 tcaatcactg gcgctggatc tggcggagta cggcattacg gtgcattcac tgatgctcgg
    24181 taacctgctg aaatcgccga tgttccagtc actgttgcca caatacgcga ccaagctggg
    24241 tatcaaaccg gatcaagtcg agcagtatta catcgacaaa gtaccgctca aacgcggctg
    24301 cgattatcaa gatgtgctga atatgctgct gttctacgcc agtcctaagg cgtcgtactg
    24361 caccggacag tcgatcaatg tcaccggcgg tcaggtgatg ttctgatcaa cagcggagat
    24421 ccattaagga tctccgtgag actatagaat gcctgatgcg ctacgctcat caggcataca
    24481 ggacttccgc cactacatta aggaaaagtt atggtatccg cactcatcac cgtcgccgtt
    24541 atcgcctggt gtgcgcaact ggccttaggc ggctggcaaa tttctcgttt taaccgtgcc
    24601 ttcgacacac tatgccagca agggcgggtt ggcgtgggcc gttccagcgg gcgctttaaa
    24661 ccgcgggtcg tggtcgccat cgcgctggac gatcagcagc gcatcgtcga caccttgttt
    24721 atgaaaggac tgaccgtctt cgcccgaccg caaaaaattc ccgcaattac cggtatgcat
    24781 gcgggtgatt tacagcccga tgtgatcttt ccccatgatc cactatcaca gaatgctcta
    24841 tcattggcgc ttaaactgaa acgtggataa tttcgttgtg aatgttactt gcttgcgaag
    24901 ttatcatttt gaaacctaaa tcaggtaatc acgcccatga aacctcgtca gcgtcaggcc
    24961 gccattctgg agtatctgca aaagcagggt aaatgctcgg ttgaagaatt ggcgcaatac
    25021 tttgacacca caggcacaac cattcgcaaa gatctggtca ttctggaaca tgccggaacc
    25081 gtcattcgta cttatggcgg agtggtgttg aataaagagg aatccgatcc gcctatcgat
    25141 cataaaacac tcatcaacac ccacaagaaa gagctgattg cagaagctgc cgttagtttt
    25201 atccatgatg gcgattcgat cattcttgat gctggcagta ccgttttgca gatggttccc
    25261 ctgctctcgc gctttaataa catcacggtg atgaccaaca gcctgcatat cgtcaatgcg
    25321 ctatccgaac tggataacga acaaactatc ctgatgccag gcggaacgtt tcgcaaaaaa
    25381 tcggcctcat ttcacgggca actggcagag aatgccttcg agcatttcac cttcgataaa
    25441 ttgtttatgg gcaccgacgg catcgatctc aatgcgggcg taaccacctt taacgaggtt
    25501 tataccgtca gtaaggcaat gtgcaatgcc gcgcgcgaag tgattttgat ggcggactca
    25561 tcaaagtttg gccgtaaaag ccccaacgta gtttgcagtc ttgaaagcgt cgataagctg
    25621 attaccgacg caggtatcga tccggcgttt cgtcaggcgc tggaagagaa agggatcgat
    25681 gtgatcataa ccggagagag caatgagtga agcactactg aacgcgggac gtcagacgtt
    25741 aatgctggag ttgcaggaag caagccgttt accggaacgt ctgggcgatg attttgttcg
    25801 cgccgccaat atcatcctgc actgtgaagg caaagtggtg gtttcgggaa ttggcaaatc
    25861 gggccacatt ggtaagaaaa tcgccgcaac gcttgccagt accggcactc cggctttttt
    25921 tgtccatccg gcagaagcgc tgcacggcga tctggggatg atcgaaagcc gcgatgtgat
    25981 gctgtttatc tcttactccg gtggcgcgaa ggaactggat ctgattattc cgcgtctgga
    26041 agataaatct atcgcgctgc tggcgatgac cggcaaaccg acgtcaccgc tgggcctggc
    26101 ggcaaaagcg gtgctggata tctccgtaga acgcgaagcc tgcccgatgc accttgcgcc
    26161 gacctccagc accgtcaata ccctgatgat gggtgacgcg ctggcgatgg cggtcatgca
    26221 ggcgcgcgga tttaatgaag aagattttgc ccgctcccac ccagccgggg cactgggcgc
    26281 tcgcttgctg aataaagtgc atcatctgat gcgccgtgac gatgccatcc cacaggtggc
    26341 gttaaccgcc agcgtgatgg atgcgatgct ggaactcagc cgcaccggtc tggggctggt
    26401 ggcggtatgt gacgctcaac aacaggtaca aggcgtcttt accgacggcg atttacgtcg
    26461 ctggctggtt ggcggcggcg cactcaccac gccagtcaat gaagcgatga cggtcggcgg
    26521 caccacgttg caatcgcaaa gtcgcgccat cgacgccaaa gagatcctga tgaagcgcaa
    26581 aatcactgcc gcaccggtgg tggatgaaaa cggcaaactc accggcgcaa taaacctgca
    26641 ggatttctat caggccggga ttatttaatc cttcaatccc agacgtttcg ccagccgatg
    26701 caggttggcg acgtcggttt ccagcatccg cgcgcaggca gcccagttgt gatgattttg
    26761 tgccagtgcc tgacgaatag tttcacgctg gaacgcttct gtcgcttcac gcaggttttg
    26821 cttaacaacg ggcaccgccg ccacttctgg cgtcggcaac gtcacctcag gaaaagcaaa
    26881 atgttgcgcc tcaagaatca cttcatcgcc gctgcgggtg gctctcgcca gaactaccgc
    26941 ccgatgaata gcatgttcca gttcgcgcac gtttcccgga aaactgtagt gttgcagtaa
    27001 atttcgcgct ccggcactta ataccacgcg ggagagcccc tgccgcaaac gacactgctc
    27061 gcagaaatac cccgccagca gaatgacatc atcgccccgc tcacgcagcg gcggcaccga
    27121 aagtggaaac acgctcaggc gatgaaacaa atcggcgcgg aatcgccctg ccagcacctc
    27181 ttcgcgtaaa tcgcggttag tcgccgccag cacgcgcaca tcgacccgca aacaacggtc
    27241 atcgccaacg cgctgaatat cgccatactg caacaccctc agcagcttgg cctgcaatgc
    27301 caacgacaac tcgccgatct catccagaaa cagcgtgccg ttatccgcca tttcaaactt
    27361 cccgctgcga ttactgatag cgccagtaaa cgctcctttc acatgcccga acaactcact
    27421 ttccgccaca ctttccggca gtgcagcaca gttgagatag accagcggat tcaccgcccg
    27481 tggcgaggct tcatgaatcg ctttcgccac cagctcctta ccggttccag tctcaccgct
    27541 gatcaggacg ttgagatcgg acgccgccac aatctcaatc tcttttttca gttgcgtcat
    27601 gccaggggac aagccaatca tctgcgtctg tttcaccgct tcaaacggcg tggcatcgcc
    27661 tggcagcata ttctggcttt ccagttgttc aatcagcaac gcattgctta acgctcccgc
    27721 cgccagcgca gcaatcagcc gtagctcttc gtcgctgaaa acatcgaact gatcgggctg
    27781 catcccgtcg agcgtcagtg cgccgatcag gttttgcccg gcaaacaatg gcagaccaac
    27841 gcaggcgtga accttcagac tctcctgccc aggaatcaaa ccgtcatagg gatcgggcaa
    27901 ttcgctgtct gcgggaaagc gcaccacatc cccggcgcgg gcaatcgctt ccagccgtgg
    27961 atgcccttcc agcgcaaagc gtctaccgag tacatccttt gccagaccgt cgatggcaag
    28021 cggaataaac tgccgcgaat cgtaacgtag caacgcagac gcatcgcact ccagcacctg
    28081 acgtagcgtg gtgatcaggc gctgaaaacg atcctggtga ccaatcccac gctgcaattc
    28141 gatggcgata ttcgccagca catcaacgga aaaactcatc tttgcctcac tgtcaatttg
    28201 actatagata ttgtcatatc gaccatttga ttgatagtca ttt
//
LOCUS       DAJOSY010000056        28187 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJOSY010000056 DAJOSY010000000
VERSION     DAJOSY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.056"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OHN27_002498"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OHN27_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2286912.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="OHN27_002499"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="OHN27_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCQ2286913.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="OHN27_002500"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="OHN27_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCQ2286914.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="OHN27_002501"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="OHN27_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCQ2286915.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="OHN27_002502"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="OHN27_002502"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCQ2286916.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIILIDYTDFVRLTVKHPSQMAW"
     gene            complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="OHN27_002503"
     CDS             complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="OHN27_002503"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCQ2286917.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="OHN27_002504"
     CDS             complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="OHN27_002504"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCQ2286918.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIADGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRAWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4523..5245)
                     /locus_tag="OHN27_002505"
     CDS             complement(4523..5245)
                     /locus_tag="OHN27_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCQ2286919.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRSVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="OHN27_002506"
     CDS             complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="OHN27_002506"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCQ2286920.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6445..6663
                     /locus_tag="OHN27_002507"
     CDS             6445..6663
                     /locus_tag="OHN27_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCQ2286921.1"
                     /translation="MQDLSLETRLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="OHN27_002508"
     CDS             complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="OHN27_002508"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCQ2286922.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7397..7597)
                     /locus_tag="OHN27_002509"
     CDS             complement(7397..7597)
                     /locus_tag="OHN27_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCQ2286923.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="OHN27_002510"
     CDS             complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="OHN27_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCQ2286924.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSRLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADENFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="OHN27_002511"
     CDS             complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="OHN27_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCQ2286925.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLNNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10099..12012
                     /locus_tag="OHN27_002512"
     CDS             10099..12012
                     /locus_tag="OHN27_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2286926.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12012..13034
                     /locus_tag="OHN27_002513"
     CDS             12012..13034
                     /locus_tag="OHN27_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCQ2286927.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13028..13246
                     /locus_tag="OHN27_002514"
     CDS             13028..13246
                     /locus_tag="OHN27_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCQ2286928.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13300..14169
                     /locus_tag="OHN27_002515"
     CDS             13300..14169
                     /locus_tag="OHN27_002515"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCQ2286929.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14224..14628)
                     /locus_tag="OHN27_002516"
     CDS             complement(14224..14628)
                     /locus_tag="OHN27_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCQ2286930.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVGA"
     gene            14930..15562
                     /gene="crp"
                     /locus_tag="OHN27_002517"
     CDS             14930..15562
                     /gene="crp"
                     /locus_tag="OHN27_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCQ2286931.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15613..17703
                     /locus_tag="OHN27_002518"
     CDS             15613..17703
                     /locus_tag="OHN27_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCQ2286932.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17770..18990)
                     /gene="argD/dapC"
                     /locus_tag="OHN27_002519"
     CDS             complement(17770..18990)
                     /gene="argD/dapC"
                     /locus_tag="OHN27_002519"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCQ2286933.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19076..19639)
                     /gene="pabA"
                     /locus_tag="OHN27_002520"
     CDS             complement(19076..19639)
                     /gene="pabA"
                     /locus_tag="OHN27_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCQ2286934.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19671..20273)
                     /locus_tag="OHN27_002521"
     CDS             complement(19671..20273)
                     /locus_tag="OHN27_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCQ2286935.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20263..20430)
                     /locus_tag="OHN27_002522"
     CDS             complement(20263..20430)
                     /locus_tag="OHN27_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCQ2286936.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20535..21107)
                     /gene="ppiA"
                     /locus_tag="OHN27_002523"
                     /gene_synonym="rotA"
     CDS             complement(20535..21107)
                     /gene="ppiA"
                     /locus_tag="OHN27_002523"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCQ2286937.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21378..22559
                     /gene="tsgA"
                     /locus_tag="OHN27_002524"
     CDS             21378..22559
                     /gene="tsgA"
                     /locus_tag="OHN27_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCQ2286938.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22821..25364
                     /gene="nirB"
                     /locus_tag="OHN27_002525"
     CDS             22821..25364
                     /gene="nirB"
                     /locus_tag="OHN27_002525"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCQ2286939.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVLCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25361..25687
                     /gene="nirD"
                     /locus_tag="OHN27_002526"
     CDS             25361..25687
                     /gene="nirD"
                     /locus_tag="OHN27_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCQ2286940.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25813..26619
                     /gene="nirC"
                     /locus_tag="OHN27_002527"
     CDS             25813..26619
                     /gene="nirC"
                     /locus_tag="OHN27_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCQ2286941.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26638..28011
                     /gene="cobA"
                     /locus_tag="OHN27_002528"
     CDS             26638..28011
                     /gene="cobA"
                     /locus_tag="OHN27_002528"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCQ2286942.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6826 a         7589 c         7105 g         6667 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcac gacctttggt
      301 cagagaacgc agctgagttg cgtatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttccgg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcagac cgatagcagc agcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtaaacac ggaagaaggt
     1321 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgttcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca tgcggtccat tttgttaacg aacgcaatgc gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagcgcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccgccac ggatcaggat
     3121 cacggagtgc tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggggtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atgagaatga tgtcgttcga aatttgacca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agggcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt accgtcaact
     3661 gcggcagtta cgccatcttg caataatagc agttcgtctc cttcactgag cagacgcagc
     3721 agcgcagcaa aatccgtcag ccagggtgag cgatgtaatg tgtgcagcat aaacgcctca
     3781 aaacctcaaa ataacatcgt agttggcgag ttcgcggcgt aaggcatctg cttcgagcgg
     3841 cgtggcttcg acaacaaagg gtgtctgcgg atctaacccg cgttcgcgca gtgaagccgc
     3901 acaaacccag cactgttcaa tgtcgtacag acccaacaat ttaaaagtgg caatgtaatc
     3961 acgcgccagc actgcatcgg gcttttgtcc tggcagcagc tgaaaaacgc catcagctat
     4021 aaagaagaca gccagatcgt cagttaatgc ggaagttgcc agtaaagcat ctaaaccttc
     4081 ccggcctgcg gctgtaccat gaggtgcagt agaaaaaaca aacgcaattc gtttcatcag
     4141 aactgtacca ccctgtcaca ggtcagcgag gcttccgcca gcgccccaag tccgcttaag
     4201 gtaaatccct gctgaaggtt tgacgaagcc agccccagtc ttccggcctc cgtttcatca
     4261 acaacgccac ggcgtaatgc tgccgctacg cagatattca gcgccacacc atgttgcgca
     4321 ttcagttgtt gccaggcccg tacgaggtca aattcgtcac ttgccggaga ggtcaattgg
     4381 ttagcgttat agaccccttc ccgatagaaa aagacgctgc ttaactcatg gccatctgct
     4441 atcagcgcct gcgcaaactg aaaagcacta ctcgcctgtt gcgtaccgta tgctggcccg
     4501 gtcaccacga tggcaaaacg cattacttat cttgcccctg gaaatcaccg ctcttgaact
     4561 ggcggatgta gagatagaca gtgtgtttgg agatgttcag gcggtcagca acctggttga
     4621 tcgcatcttt aatatcgaag atccctttct cgtagagatt cagcacgatc tgacgatttt
     4681 tggcgttatt agaaacactg cgatcggcat tcacttcttc gatggtgaac tccagcgttt
     4741 gggtaaccag atcttcaaca gaagaggcaa agttgacgct tgaaccgaca tccggggttt
     4801 ctggcggcac aaaggtgctc ataatctgcg agaagggaac atcaagattc atattgatgc
     4861 acagcagacc aattacacgc tgttcgcggt tacgaatcgc gatagtcagg gacttcatta
     4921 atacgccgct tttggcgcga gtaaagtagc atttagaaac gctgctatcc gctcccgtca
     4981 tatcgtgcag catacgtagc gccaggtcag taattggcga accaatcttc cggcctgtat
     5041 gttcaccgtt agcaatgcga atggctgaac attttagatc ctgcaaagag tgcaaaacga
     5101 tttcacagtg ggagccaata agcatcgcta acccgtccac caccgcttcg taggatttca
     5161 gaatatcaaa atcggtctgg tcgaaaggac gttgatccag taaatccaac tcactggttt
     5221 cgttggttaa aagcgacctg gacatgaaaa aaaacactcc ttttcaggag cctgtcgtta
     5281 acttttcagg gcaggctcat taatgatgcg ggtaactaaa ttaatacagc ggaggttccg
     5341 ctttccagca ctaattatat ccggcctgta ataaaaaaac cgccgcctgg tcaggcggcg
     5401 gttcttaatg cttatttttt agcagaatct gcggctttcg catcagcttc cggctttgca
     5461 tcagccttcg gcgctggttt cacatccagc agctctacgt caaacaccag ggtagaattc
     5521 ggtgggatcc ccggaacacc cgctttgccg taagccagtt ctggtggaat aaccagtttg
     5581 atcttaccgc ctttcttgat gttcttcaga ccttctgtcc aacccgggat aacaccgtcc
     5641 agacggaaag aaagcggttc accacgggtg taagagttgt cgaactcttt accgtcgatc
     5701 agcgtacctt tgtagttcac tacaacagta tcgctgtctt tcggtgcttc gcctttaccg
     5761 gcttctacta cctgataaac cagaccagtt gaagaggttt tcacaccttt ctctttggca
     5821 aatttctcgc ggtactcttt accttttgct tcgttatcag ccgcgtcttt ttccatcttc
     5881 gcctgagcag aagacttcac gcgagcttcg aatgcttgca gagtctgttc gatctcttgg
     5941 tcggagagtt tgctcttatc agcaaatgca tcctgaacac cagcgatcag ctgatcttta
     6001 tccagtttga tgcccagttt ttcttgttct tttagagagt tttccatgta acgacccagc
     6061 gaggcaccca gtgcataagc tgatttctga tcgtcatttt tgaacgctgc tttgctgtca
     6121 gcagttgtag caggttttgc agcttcagca gcaaaagtga ttggtgcatg cagggcaacg
     6181 gccattgtgg tcgccagcag cgttacttta aacagtgatt tcatccatat ctccaagatc
     6241 ggggcatctc accccagggt taactattat cagaagggta ctataaagcg ttgtcgaaca
     6301 aatctacata cagacacgcc ctattatcat ctattttcag actctttttg tttaaattag
     6361 tttcgatgac cgcgaaatga gtgctgtctc gggcagcaaa gttaagtaga atccgcggcg
     6421 accattcgac aaaagaggtg aatcatgcag gatttatcat tggaaacacg cctggcagag
     6481 ctggagagcc gactggcttt tcaggaaatc accattgaag aactgaacgt cacggtgacc
     6541 gctcatgaaa tggagatggc gaaactgcgc gatcatctgc gtctgctgac cgagaagtta
     6601 aaagccagcc agccgtcgaa catcgcgtcg caggctgagg aaacgccacc gccacattat
     6661 tgaggcgtaa aaaaagcggg gattccccgc ttttttgtca ccttttcggt attagtggca
     6721 accacaaccg ccgttgcctt taccgccaca gcaaccttcg ccaccgtgtt catgaccgtg
     6781 atcgtggcca tgaccgccgc agcaaccgtc gtggtcgtga tcgtggtgat gatcgtgcgc
     6841 gccgtgaacg tgaccatgag ccagttcttc ttcggtcgct tcacgaatcg ccacaacctc
     6901 aacgttgaat ttcaggttct gaccggccag catgtggtta ccatcaacca ctacgtgatc
     6961 gtcttcaacc gcagtgattt caaccggtac cggaccctgg tcggtttcag ccaggaaacg
     7021 catacctacc tgcagttcat caacgcccat aaatacgtct ttaggaacac gttgcaccag
     7081 gttttcgtcg tactgaccgt aagcgtcgtt cgcgccaaca gcgacatcaa atttgtcgcc
     7141 aacttcatga ccttccagcg ccgtttccag gccagagatc agggaaccgt gaccatgcag
     7201 gtagtccagc ggcgcactca ccggagactc atcaaccaac acaccgtctt ctgtacgtac
     7261 ctgataggcc aggctgacca ccaggtcttt tgctactttc atgatatctc ctgagcatgg
     7321 gaagaatagt ggcgcagatt gtagcggaat tctgcagccg tgtactcact agcttaaaaa
     7381 aactcggcgc atatcgctag tccggatgaa aaatcccgat cacttgctca tctttgcgaa
     7441 cgtgatcgcg ggcttctttg tctgcttctc gcatctgatg tccgcactta acgcattcaa
     7501 caatatcaat attattttcg cgccacatcg ccattgaatc ctgtgcctga caggccgggc
     7561 attttgcgcc cgcaataaaa cgttttcgga ttgccatctt taccctctac tcaaattcat
     7621 cccagccgtc cagctggcgt cgttcttgtt gcatttcacg ctggaaaatc tcttccagtt
     7681 cgcgtcgggc ttccctggcg cgagaaattt gtacagtatc ggcatgcatt gggatgagct
     7741 ctcgcagcat ccgcatatcc aggcggcgaa aatgcagttg cgcccgctgc gcctgatgcg
     7801 gatgcatgcc aagcgtgacc agcgtcttgc gccccagctc taacgcactg gagaatgttt
     7861 cacgggaaaa ctgcgtcacc cctgcctgta ataactcatg cgcttccaca cgtccgcgcg
     7921 ctcgcgcaag aatatgcaaa tgcggaaagt gctgctggca tatttccacc agcttcatgg
     7981 tgtcttccgg ttcgttacag gtgatcacga tagactcagc ggcctctgca cccgcagaac
     8041 gtaaaagatc gacctgcgtg gcgtcgccgt aataaacttt gtagccgtat ttacgcatca
     8101 ggttaacggc gctgatatcc cgctccagca cggtaatgcg cattttattt gccatcagca
     8161 aacgaccaat cacctgacca aaacgcccga agcccacgac aatgacctgg ggtttatcat
     8221 cgttgaccca cggtttttcg tcttcttctt ccggtccgtt aaactggcgg gatagccatt
     8281 tatccaccag cttcatcagc aacggcgtgg tcatcatgga aagcgtcacc gtcaccagca
     8341 acagcgccat ctggtcgccc tggaataagc gttgcgaaga agcggtagaa aagaggacaa
     8401 aggcaaactc gccgccctga ctcaacacgc cagcaaactg catccgctct gagctacgca
     8461 cgccatacaa tcgcgccagc agatacaaca cgagaatttt caccgccacc agcacaacca
     8521 cgcttatcac tacccacaac agatgggtat aaagcacccc gagattgagc gacatgccga
     8581 cagagataaa gaacaaaccg agcagcaagc ctttgaaggg atcgatagcc gtttccagtt
     8641 catggcgata ttcactttcc gccagcagca cgcccgcaat aaacgtaccg agcgccatcg
     8701 acagccccag cgcatccata aacaatgcgg aacccaatac cagcagcagc gtcgcggcgg
     8761 tgaacacttc ccgcacgcca gaagctgcaa taaaacggaa taccggacgc aataaatagc
     8821 gcccgccaat cagcatgccg acaaacgcca gcaccttcat gccgatcttc atccagtcga
     8881 aattttcgtc tgccgaaccc gccaacaacg gcactaacgc cagtgccggg atcaccgcca
     8941 gatcctgaaa cagcagaacc gaaaaaccta gctgcccgga ttcgctgcga ttcatccctt
     9001 tctcacgcat caattgcaac gccattgcag ttgaagacat cgcaaggcca atgccaccga
     9061 ccaccgccgc ctgccaggcg aaatccgtca gcatcaataa tcccgccagc aacgccgcgc
     9121 ttaacagcac ctgtgccgcg cctacgccaa aaatcgaacg ccgcagttgc caaagcctgg
     9181 agggattcaa ctcaaggccg atgataaaca tcaggaacac cacgccgagt tccgaaaagt
     9241 ggaggatctc atcgacgtcg ctaataaacc ccagccccca cgggccaatt gcaatccctg
     9301 ccagcaaata tcccaacaca gcgccaatac ccagccgcga tgccaacggc accgcagcca
     9361 ccgccgcgaa gagaaacagc actcctgcaa gtaaaaaatc ggaaccttcc atcagcggcc
     9421 tcctggagac agcggatttg ccagccagtc accgtaggct ctggcgtggc tcgccagctc
     9481 ctgtgcgctt tgccgtctcg cccagtaaat aatgatggga cttaaccaat gcatccggca
     9541 catgcccgcc gccagttcaa aggggcgcag aacatcgctc atcgggtagc gattcaatgc
     9601 gtcataacgg taagcacttt ccggctcgcc ggtggtaatc acgctacgcc agtactttcc
     9661 cgccagttgg tttcctcctg gcccgctggc aaaaccacgg cttaataccc ggtccagcca
     9721 ctctttcagt agcgccgggc agctataggt ataaagagga tgctgaaaga caatcacctc
     9781 gtgctcgcgc agtaatgcct gctcacgggg gatatcaata aaaaaatcgg gatagtgcgc
     9841 gtaaaggtcg tgcacggtaa cattgttgag ctgcgtagcc ggtttaagca gcacccggtt
     9901 tgccaccgag tcctgagatt ccggatgggc atacagcagc aaaactttcg ctggctgaga
     9961 catcatcccc ctcccggtat ggtttttgtg tatagtcgct gttttgggct accattgcgc
    10021 ccggtgcggc agctcgccca tacattacat tacattatca taatgataag ttaacatagt
    10081 ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg cggcgtgcgc
    10141 gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg cctggtgggt
    10201 aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat cagcgccgac
    10261 ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca ggaaacgccg
    10321 gcgttaccgc aagcggcgct ggaatatgtc attgacggag accgtgaata tcgtcaactg
    10381 gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc gaccattcat
    10441 ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag cctgctgcac
    10501 ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc cggtggctgg
    10561 cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct gctgctcgac
    10621 gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg gctgaagagc
    10681 tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc gatcgtcgat
    10741 aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta cagttcgttt
    10801 gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag ccagcaggaa
    10861 cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac caaagcgaag
    10921 caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc cgcgcacgtc
    10981 gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc gttactgaag
    11041 atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat taaactgaac
    11101 ctggtccccg gctcgcgcat tggtctgcta ggccgcaacg gcgcaggtaa atcgacatta
    11161 atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct ggcgaaaggg
    11221 atcaagctcg gctacttcgc ccagcatcaa cttgaatact tgcgcgccga cgaatcgcct
    11281 attcaacatc tggcacgttt agcgccgcag gagctggagc aaaaactgcg tgactacctc
    11341 ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt ctccggtggg
    11401 gaaaaagccc gcctggtgct ggcattaatc gtctggcagc gtccgaatct gctgctgctc
    11461 gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga agcattaatc
    11521 gagttcgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg ttccaccact
    11581 gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga tctggaagat
    11641 tatcaacagt ggttgagcga cgtacaaaag caggaaaacc aggccgacga agcgccaaaa
    11701 gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc ggagttgcgt
    11761 gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat ggagaagctg
    11821 aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta tgaccagagc
    11881 cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc cggcctggaa
    11941 gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgtt gctggaaggc
    12001 caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc agcagtgctg
    12061 aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg ccgcgtctgt
    12121 ttcgtcgcaa ggtgaaattc accccgtact ggcagcggct ggagttgccc gacggcgatt
    12181 ttgtcgatct cgcgtggagt gaagccccag cacaggcgcg gcataaaccg cgtttagtgg
    12241 tatttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg gttgaggcgg
    12301 cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc ggtgaaccaa
    12361 accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg tttttacgct
    12421 ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg ctcggcggta
    12481 atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat gcggcggtga
    12541 ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag ggcttttccc
    12601 gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc aagctggcag
    12661 cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt cgcatccgtg
    12721 aatttgacga tctcatcacc gccagaattc acggctacgc tgacgctatc gactattatc
    12781 gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg attattcacg
    12841 ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt ctccccccgc
    12901 aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc ggtacattac
    12961 ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg tatctggagg
    13021 cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa atttaattga
    13081 aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac ttgaacagaa
    13141 agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat ggtcggagct
    13201 gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg caaccgtcgg
    13261 cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa acatccggtc
    13321 attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc gtttcgtaaa
    13381 atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt tcaccgttac
    13441 acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg gcatatcagc
    13501 tacttcggcc ccgaggctaa cgacttcggc ctgctggaac aaaccttcat tgaatacggt
    13561 cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc cgtaccgtgg
    13621 aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac tgatgtactg
    13681 ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc gcagcatgtg
    13741 gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa actgatccgc
    13801 gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt gcgttcaatg
    13861 gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa cttccagcgc
    13921 gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc gctcgatgaa
    13981 agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg gctgctggcg
    14041 atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg cggcaaaatg
    14101 ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga aggaaagaaa
    14161 atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa cgggatagtt
    14221 aaatcacgcg ccaaccactt catacgagtg agtaatattc accgcttttt ccagcatcaa
    14281 cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg ctgcgtcttt
    14341 caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc gtggtgcctc
    14401 ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac gccctttttg
    14461 caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca ccatttccat
    14521 cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac cagaggcaga
    14581 ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt ttgctactcc
    14641 actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac ggaaggcgac
    14701 ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc taaaacagtc
    14761 aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca cattaccgtg
    14821 cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa tccagagaca
    14881 gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca tggtgcttgg
    14941 caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca ttcataagta
    15001 cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt actacatcgt
    15061 taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga tcctctccta
    15121 tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc aggaacgtag
    15181 cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca aaaaatttcg
    15241 ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga tggcgcgtcg
    15301 tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga cgggccgcat
    15361 tgcacagact ctgctgaatc tggcaaaaca accagacgct atgactcacc cggacggtat
    15421 gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc gtgaaaccgt
    15481 gggacgcatt ctgaagatgc ttgaagatca gaacctgatc tccgcacacg gtaaaaccat
    15541 cgtcgtttac ggcactcgtt aatcccgtcg gagaggcgcg ttacctggta gcgcgccatt
    15601 ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta tgcacttcga
    15661 caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga attacgattc
    15721 ggtctgctct tctccctcgt tcctgcctgt tgcaatattg cgggccttga tacgcctcat
    15781 aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg tagcttactg
    15841 acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg attaacgctg
    15901 gtacttggcg tcactgctga gctggggcca ttgcacgcaa aattgcttcc tgcatcgctg
    15961 ctcgccgcca tttttaccct cagtttggcg ggatacatgc cggtctggga accgttgctc
    16021 atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt ctggatctgg
    16081 cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc agattattgt
    16141 gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct gccgccgctg
    16201 ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca aatgcatatg
    16261 ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca ggaggcgctg
    16321 gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca aaagctggtc
    16381 gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc cgctcgcctg
    16441 cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac gatggaaaag
    16501 caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt tgggcaattc
    16561 tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc gctctatgcc
    16621 cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct gaaaagttat
    16681 ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat gttaagcgtt
    16741 gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct gatgacggta
    16801 ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa tcgctccgtg
    16861 ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat tcccgaaggt
    16921 tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt gcgcaaaaac
    16981 tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca actattgtgg
    17041 ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg ttgtttaatt
    17101 gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt gcgtaaaaac
    17161 gcccatgatg ctttagaagc ctatcaggaa gcgattcgct tgattcttag cgaggatccg
    17221 caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa cactctgtat
    17281 aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct ggcagatatg
    17341 aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat gaccacgctg
    17401 gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca gtcttgtgaa
    17461 atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag ttctggcgat
    17521 gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg tacgctggag
    17581 cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc gtcgatggca
    17641 tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga ttcgaaggcg
    17701 taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct tttgcctgat
    17761 gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt gcatcccttc
    17821 atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat ccggtccggc
    17881 attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg cccgaccttt
    17941 gtactgtggt ttcagctctg cgccaatcaa cagccccata ccgcgaatat cgctaaatac
    18001 atcgtactgc tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt tcgcctgaat
    18061 gccttccagc acttcagggg tattgatgat atcaaacgcc gcccccgcta ctgcacaggc
    18121 cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag aagcaatttc
    18181 cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg cagaggtcag
    18241 aatatccggc gtaacgccgt agtgcatgta agcaaacaaa tcgccggtcc gccccatccc
    18301 gcactgcact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct cgcgcaagcc
    18361 ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg gctcaaccac
    18421 caccgcacag gtgtgatcat ccatcaccgc tttcactgca tggagatcgt taaagggaac
    18481 gtggatgatg tctgccggtt tcggcccaaa gccgtcggaa tattttggct gcccacccac
    18541 cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa ttttggtttt
    18601 gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg tttcgttagc
    18661 ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gttgcctcaa tcagtttacg
    18721 cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca gagtttcgcc
    18781 ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg taactgcaat
    18841 gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc tgccctgacc
    18901 ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat cgaaagtcgc
    18961 gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg cataaacaaa
    19021 tgtgaaatta taaccataaa atatgcataa aaaatcacta aatggcaatc agaaatcagc
    19081 gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt tctggatgga
    19141 actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg cgggtttcgc
    19201 tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc agcgaatggt
    19261 agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg ttatgtgtaa
    19321 tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg ccaaatgcct
    19381 gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg gcatagtggc
    19441 gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt gagatgacaa
    19501 ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg cgcttaacca
    19561 gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa gaatcgtagt
    19621 tatctataag caggatcatg gcggctccgg tacaaaagaa ccgcgctatt ttactcagat
    19681 tccccggctt cgcttaccac tttgctaaat atcatctgca gtgcggtgag atcccccatt
    19741 gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca gctcagttga
    19801 tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc acttcccacc
    19861 cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc gacgaacttc
    19921 gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag ggcattgccc
    19981 tctttttcga tataagcaaa gtggcagaac ggcgtatcac cctgataaat atcaacttca
    20041 cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat agtgcgcaaa
    20101 tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag cgccgtcatt
    20161 tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt acgcatgata
    20221 tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct catagtgcct
    20281 cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta aaggcatctc
    20341 gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct cccagagacg
    20401 ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg tccatttgcc
    20461 acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg caagattgcc
    20521 cgcgcgaaaa atcattacgg caggacttta gcggaaagga taactaccgg ttttgacggc
    20581 acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc ggcaacgtcc
    20641 atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc atggtcaagg
    20701 aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt gtcagcggta
    20761 cgtgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc atttttgatt
    20821 ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg aatcataaag
    20881 ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac ataatcgaca
    20941 aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat gttaccagct
    21001 gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg agaaagagca
    21061 gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag atttccttaa
    21121 caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag aacaaggcca
    21181 tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac tttttattcc
    21241 gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa acatttaaac
    21301 agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc cattttattt
    21361 ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg attagctttc
    21421 tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg ggaaatatcg
    21481 ccgattattt caatctgcct gtttccagta tgagtaatac cttcaccttc ctcaacgccg
    21541 gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg ttgaaaacgc
    21601 agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg ttcagccaca
    21661 gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc atcaccatgt
    21721 cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt tcccgcctgt
    21781 tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc gccgcgtttc
    21841 tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg gtgtatgtcg
    21901 ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat gcgccaaaaa
    21961 cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc tctgttgcgg
    22021 cgttgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag tatgccaaag
    22081 gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg atgtcataca
    22141 tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa cgcattctga
    22201 ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga acaccagcac
    22261 atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat accaccatca
    22321 tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac tttgtcctga
    22381 cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatc gttgaacata
    22441 gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc tttgtgatgt
    22501 gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc tctcattaat
    22561 tgctcatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc cgctacgtgc
    22621 atctatttct ataaacccgc tcattttgtc tattttttgc acaaacatga aatatcagac
    22681 aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag gagtatataa
    22741 aggtgaattt gatttacatc aataagcggg attgctgaat cgttaaggta ggcggtaata
    22801 gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt aacggtatgg
    22861 tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac tttgatatta
    22921 ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg tcttacttct
    22981 ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag aaacacggca
    23041 tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag gtgattcact
    23101 ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt tcctacccgt
    23161 ggatcccgcc aatcaaaggt tctgatactc aggactgctt tgtctatcgc actattgaag
    23221 acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt gttggtggcg
    23281 gcctgttagg tctggaagcc gcaggcgcgc tgaaaaactt aggtattgaa acccacgtta
    23341 tcgaatttgc ccctatgctg atggcagaac agcttgatca gatgggcggc gagcagctgc
    23401 gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc cttgagattg
    23461 tgcaggaagg tgttgaagcg cgtaaaacca tgcgttttgc cgacggcagc gaactggaag
    23521 tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca acccagtgtg
    23581 gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag acttccgatc
    23641 cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt ggtctggtag
    23701 cacctggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc gaaaacgcct
    23761 ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta ggcggtattg
    23821 gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac gaaagtaaag
    23881 agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt gcggtactgg
    23941 tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct atcgaactgc
    24001 cggaaaaccc ggattccctg atcctgccag cacactcggg tagcggcaag ccgtctatcg
    24061 gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc accaaaggtg
    24121 atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa gctgaaacca
    24181 aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg aacgcggaac
    24241 tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct tattcgcgtc
    24301 aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa ctgctggcga
    24361 aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg ctgctggcct
    24421 cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat tctaacgaca
    24481 acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt tctccgggcg
    24541 gtgaaatcac cccggaaggg ctgatggcgg taggtcgtat cgcgcgtgaa tttaatctct
    24601 acaccaagat cactggctcc cagcgtctgg cgatgtttgg cgcacagaaa gacgatctgc
    24661 cggagatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc tatgcgaaag
    24721 cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc gttggcgaca
    24781 gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg ccgcacaaaa
    24841 tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt aaagacgtgg
    24901 gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc ggcatgaaac
    24961 cgcgtcatgc ggatctgctg gcggcggata tcgatcgcga aacgctgatc aaatatctcg
    25021 accgcttcat gatgttctac atccgtactg ccgacaaact gacgcgtacc gcaccgtggt
    25081 tagaaaacct cgaaggcggc atcgattacc tgaaagcagt gatcattgac gacaaactgg
    25141 ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta ctgtgtgagt
    25201 ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc atcaacagcg
    25261 acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt ccggcaacgc
    25321 cgtatgaacg tatcccagta actctggtgg aggacaacgc atgagccagt ggaaagacat
    25381 ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag gtgacgagca
    25441 ggtcgcgatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca acatcgaccc
    25501 gttcttcgag tccagcgtgc tgtcacgcgg actgattgcg gaacaccagg gcgagctgtg
    25561 ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagc gacggcttgt gcatggaaga
    25621 cgaacagttt tccgtcaaac attacgaagc gcgagtgaaa gacggcgtgg tgcagctgcg
    25681 cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc tgtaataatc
    25741 ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt ttatttttta
    25801 aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc tgcgcgcatt
    25861 gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat ggcgggcgcg
    25921 tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga tccatccgta
    25981 cgccctttgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat tatcgccggt
    26041 tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc gggcagcatc
    26101 agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct ggtcggttcc
    26161 gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt agataccagc
    26221 atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact cttcttcaaa
    26281 ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg cactgaaggg
    26341 gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc cggctacgag
    26401 cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca cagcgaagcc
    26461 tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa tactttatca
    26521 ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa tcgtccggtt
    26581 gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg tttttacgtg
    26641 gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt cggcggtggt
    26701 gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt aacggtgaat
    26761 gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt aaccctcgtc
    26821 gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc agcgacggat
    26881 gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat cttctgtaac
    26941 gtggtcgatg cgccgaaagc cgccagcttt attatgccgt cgattattga ccgctcaccg
    27001 ctcatggtag cggtctcctc tggcggcacc tctccggttc tggcacgcct gttgcgcgaa
    27061 aaacttgaat cactgctgcc gttacatctg ggccaggtag cgaaatacgc cgggcaatta
    27121 cgcgggcgag tgaaacaaca gttcgccacg atgggtgagc gtcgccgttt ctgggagaaa
    27181 ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa agccattact
    27241 gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt ggtgctggtt
    27301 ggtgcaggtc cgggcgatgc cgggctgctg acactgaaag gactgcaaca aattcagcag
    27361 gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct ggtacgccgc
    27421 gatgcggacc gtgttttcgt cggcaaacgc gcgggatacc actgcgtacc ccaggaagag
    27481 attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg gctgaaaggt
    27541 ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg caacgcgggt
    27601 attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc ctattcgggt
    27661 attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg acacttaaaa
    27721 accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct ggtgttctat
    27781 atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg aatgccaggc
    27841 gaaatgccgg tggcaattgt cgaaaacggt acggcagtca cgcagcgcgt gattgacggt
    27901 acgctcacac agctgggaga actggcgcag caaatgaaca gtccatcgct aattattatt
    27961 ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta atttaacccg
    28021 ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa taaaacatag
    28081 ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga cttacaaaat
    28141 taagtcattc gctcactgac gccaattaaa tattcctgta tcgttct
//
LOCUS       DAJOSY010000057        28181 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJOSY010000057 DAJOSY010000000
VERSION     DAJOSY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.057"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..655
                     /locus_tag="OHN27_002529"
     CDS             <1..655
                     /locus_tag="OHN27_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCQ2286943.1"
                     /translation="NGIWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLL
                     LCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISML
                     AGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWSGIISHGR
                     NKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(691..1647)
                     /locus_tag="OHN27_002530"
     CDS             complement(691..1647)
                     /locus_tag="OHN27_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCQ2286944.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1647..2888)
                     /gene="clsB"
                     /locus_tag="OHN27_002531"
     CDS             complement(1647..2888)
                     /gene="clsB"
                     /locus_tag="OHN27_002531"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCQ2286945.1"
                     /translation="MKCSWREGNKIELLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2885..3646)
                     /locus_tag="OHN27_002532"
     CDS             complement(2885..3646)
                     /locus_tag="OHN27_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCQ2286946.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLQTWRHLSDHAPLSAEIHL"
     gene            3779..4189
                     /locus_tag="OHN27_002533"
     CDS             3779..4189
                     /locus_tag="OHN27_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCQ2286947.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4151..5257)
                     /locus_tag="OHN27_002534"
     CDS             complement(4151..5257)
                     /locus_tag="OHN27_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2286948.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5268..6401)
                     /locus_tag="OHN27_002535"
     CDS             complement(5268..6401)
                     /locus_tag="OHN27_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2286949.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6394..8130)
                     /locus_tag="OHN27_002536"
     CDS             complement(6394..8130)
                     /locus_tag="OHN27_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2286950.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8123..9118)
                     /gene="hlyD"
                     /locus_tag="OHN27_002537"
     CDS             complement(8123..9118)
                     /gene="hlyD"
                     /locus_tag="OHN27_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCQ2286951.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(9121..9792)
                     /gene="cecR"
                     /locus_tag="OHN27_002538"
     CDS             complement(9121..9792)
                     /gene="cecR"
                     /locus_tag="OHN27_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCQ2286952.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            10021..11385
                     /gene="rhlE"
                     /locus_tag="OHN27_002539"
     CDS             10021..11385
                     /gene="rhlE"
                     /locus_tag="OHN27_002539"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCQ2286953.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11617..12099)
                     /gene="ybiA"
                     /locus_tag="OHN27_002540"
     CDS             complement(11617..12099)
                     /gene="ybiA"
                     /locus_tag="OHN27_002540"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCQ2286954.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGHGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12219..14369
                     /gene="dinG"
                     /locus_tag="OHN27_002541"
     CDS             12219..14369
                     /gene="dinG"
                     /locus_tag="OHN27_002541"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCQ2286955.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14397..15359
                     /gene="ybiB"
                     /locus_tag="OHN27_002542"
     CDS             14397..15359
                     /gene="ybiB"
                     /locus_tag="OHN27_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCQ2286956.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15500..16585
                     /gene="hcxB"
                     /locus_tag="OHN27_002543"
     CDS             15500..16585
                     /gene="hcxB"
                     /locus_tag="OHN27_002543"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCQ2286957.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(16814..17074)
                     /locus_tag="OHN27_002544"
     CDS             complement(16814..17074)
                     /locus_tag="OHN27_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2286958.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17339..17605)
                     /gene="ybiI"
                     /locus_tag="OHN27_002545"
     CDS             complement(17339..17605)
                     /gene="ybiI"
                     /locus_tag="OHN27_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCQ2286959.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17679..18356)
                     /gene="ybiX"
                     /locus_tag="OHN27_002546"
     CDS             complement(17679..18356)
                     /gene="ybiX"
                     /locus_tag="OHN27_002546"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCQ2286960.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18398..20680)
                     /locus_tag="OHN27_002547"
     CDS             complement(18398..20680)
                     /locus_tag="OHN27_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCQ2286961.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLMNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSLLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(20945..21205)
                     /locus_tag="OHN27_002548"
     CDS             complement(20945..21205)
                     /locus_tag="OHN27_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2286962.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            21481..22407
                     /gene="rlmF"
                     /locus_tag="OHN27_002549"
     CDS             21481..22407
                     /gene="rlmF"
                     /locus_tag="OHN27_002549"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCQ2286963.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISSNPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(22404..24629)
                     /gene="ybiO"
                     /locus_tag="OHN27_002550"
     CDS             complement(22404..24629)
                     /gene="ybiO"
                     /locus_tag="OHN27_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCQ2286964.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLFNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(24890..25612)
                     /gene="glnQ"
                     /locus_tag="OHN27_002551"
     CDS             complement(24890..25612)
                     /gene="glnQ"
                     /locus_tag="OHN27_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCQ2286965.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(25609..26268)
                     /gene="glnP"
                     /locus_tag="OHN27_002552"
     CDS             complement(25609..26268)
                     /gene="glnP"
                     /locus_tag="OHN27_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCQ2286966.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(26407..27153)
                     /gene="glnH"
                     /locus_tag="OHN27_002553"
     CDS             complement(26407..27153)
                     /gene="glnH"
                     /locus_tag="OHN27_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCQ2286967.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(27557..28060)
                     /gene="dps"
                     /locus_tag="OHN27_002554"
                     /gene_synonym="pexB"
     CDS             complement(27557..28060)
                     /gene="dps"
                     /locus_tag="OHN27_002554"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCQ2286968.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         6667 a         7334 c         7502 g         6678 t
ORIGIN      
        1 aaacggtata tggctcatta ccgctttagg tttggtggca accgcaggac tagcctgggg
       61 agctaagtat ctcgaaatta cggcaaccaa atatgattca ccaccaatgt atgtcgccat
      121 agggttatta ttactttgta tgtatggctt aagtaaggat atcaacaaga taaatgccgc
      181 catcgcgggc gtaatatatc tgtttttact ctctttggtg gcgattgtcg ttgcaagttt
      241 agttcctgta tatgccatta tcatcgtgtt cagcactgcg ggcgcgatgt ttttaatcag
      301 tatgctggcc ggtttattat ttaatgttga tcctggttct caccgtttta tcattatgat
      361 gacgttgaca gggttggccc tggtaatcat cgtgaatgcg gcattaatga gtgaacggcc
      421 catttgggta ataagttgct taatgattgt gttatggtca ggcattatct cgcatggacg
      481 aaataagctc cttgaattgg cggggaaatg ccatagtgaa gagttgtgga gtccggttcg
      541 ttgcgctttt acaggtgcat taacactcta ttactatttt atcggcttct ttgggatact
      601 tgccgcgata gctataacgc ttgtctggca aaggcatacg cgtttttttc attagccgta
      661 aatgcacata ctaaagaact taactaaact tcacatcgcc gcttcatttt tcgcccgtag
      721 cttcttcgcc tggctttcca gcaacaggta acagatcagc gccagcagca gtgggataaa
      781 gtaatacagc acacggtaag cgagtagggc ggcgataatt gtacctttgg aggtatgctc
      841 cccagccagt agtgcgataa acaccgcttc cagcacaccg atccccgccg gaatatgcac
      901 gatgacgcca gcaatactac taaccagtaa cacgcccagt acaaagaaat agttcacgct
      961 ttgaccaagt aacagccaga taatcgcccc cattaccatc cagttaacgc tggaaatcag
     1021 catctgagcg agggcgaatt tccatgaagg cagcaccagc ttttgccctt tgatggtcat
     1081 atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc gcgataatca tcagtaagcc
     1141 aatgccgaga atacgcagcg tagtttgatc gacataccag tgatccggca actccaccac
     1201 gcctgcggta aagataatcc ctgccagtaa aatgtagccc agccagttgg tggtaatact
     1261 gagcgagaaa atccgcgtaa tagtgctacc gggcaacccc agacgagagt acaaacgata
     1321 gcgcatacca atgccgccga cccaggtact gagcgtcagg ttgaaggcgt agcagataaa
     1381 cgacaccagc atcacctggc gcttcgccag tttgtgaccg cagtaaaagc gggcgagcag
     1441 gtcatagcag ccgtaaatca gatagctgac gaccaccagc ccgaccgcac taagcagcgc
     1501 aacgcgattg tagtcgcgga tgaccttcca gacctcttcc cagtccactt ttttggcgta
     1561 gaccaccagc aacacaatca ccgcgataaa aaacagccag gtgaggatct tctttgctaa
     1621 gcgccagcgc gggtgtgatt tactcatcag ggttttaccc ccgtgttttc agtttctacc
     1681 cgatcctgcg tttccattgt cggttgtgcg ggcggatcaa cctgcgccag acgtggcgtg
     1741 tgtgccggaa gccagccaac cagcgccggg aagtggcgta aaaagtggaa cgccagcacg
     1801 cttttggtca ggttccacca ggtgcgtttg ggcagcatgg tttcatccac ctgctgacaa
     1861 tctgcggcga taatgccgtt cagattatcg cgcagcgtct ggttaaaatt acgatcgtgg
     1921 atgatgacat ttgcttcgag attcagtgac aaactgagcg gatcgagatt actggaccct
     1981 actgtcgccc agtgatcgtc catcaatgct actttgccgt ggagcgggcg gcggcggtac
     2041 tcaaacacct gaacgccgcc tttaaccaga tagttataca gcaagcgcgc gccgactctg
     2101 acaatcggca tatccggttc gccctgaatg atcagtttga tccgcacccc gcggcgtgcc
     2161 gctttacgca aggcgtgtaa aaatcgatag ccggggaaga agtaggcgtt ggcgataatg
     2221 acttcccgcc gcgcctgagt gagcattttc agatagtggc gttctatatc atcgcggtgt
     2281 tcttcgttat cgcgccagac cagcaatacc tgcgcttctc ccggctggcg attttcttcc
     2341 gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct gtccaggcag gttttccagc
     2401 tcaaactgga gaatatcttc gacaatcggc ccttcaaggc gtaccgcgta atcctgttta
     2461 gcctctggac cgtagctgga catatgctcg gcggagtaat tcagcccgcc aataaaggcg
     2521 atacgcgcgt cgatcaccac aattttgcga tgcatccggc gaaacacatt ggtgcgcata
     2581 ccaaaaaggc gagggcgggg atcgtagtag cgaaatacca cgccagctgc cgtcagttca
     2641 ttgacaaact catcgctgag atccggcgaa ccgtagccat ccagcaagac ttccgcttta
     2701 accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct gtttgccgac gtcatcctca
     2761 aaccagataa acgtttcaag aatgatgcgt tcttgtgcct cgccaatcgc cttaaacacc
     2821 gcgggataat attgctcgcc gttttccagc aactcgatct tattgccttc acgccagcta
     2881 catttcataa atgaatctcc gcacttaaag gggcatgatc agaaaggtgt cgccatgtct
     2941 gcagcggcaa cgcggttggc gcgctggcgc tggcattttt gacgtagatc ctgtccagac
     3001 gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg tccgtgggcg cgggtaaata
     3061 tctcatccag tccggcctgc acttttaacg gatgattagc tttttgccgc cagtcattga
     3121 aatcacccgc caccaatacc ggttcgccgt ccggtagctc attcacccat tcggcgagca
     3181 tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag atgtacgcac atcacatgaa
     3241 tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag cacgccgcgc ttttccgcac
     3301 catcgaccga aacatcgcga ttctcataat gttcaatggg ataacgcgac agtacggcgt
     3361 tgccgtgatg cccttccggg tatacggcat tgcgaccgta ggcaaaatcg ctccacatag
     3421 tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt ttccacatgc agcggatgaa
     3481 cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc ggcgctgacg gtacgcacgg
     3541 cgtcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc ggtaaagcct ttgtgaatat
     3601 tgatggtgag caccttgaac gaaaattgtt gtgtttgatc gggcataatt ttcctgtctt
     3661 tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa aaaggtgcgg tcttacggaa
     3721 ttttccgtaa agttcggtac tctgagtaag tagagataaa ttcttcagga gagaagccat
     3781 gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt tgctggcaga ttatgttgca
     3841 tttactggta gtggcgctgc tggtggtggg ctggatgagt aagactctgg ttcacgtcgg
     3901 cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg atgctggtgt ttcagcgcca
     3961 ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa gagctgacca cgacctggta
     4021 ttttggcgca gcgctgattg tgctgtggct gttgtcccgc gttctggaaa acaacttttt
     4081 gctggcaatt gcagggctgg caatccttgc cggcccggcg gtagtgtctt tgctggcgaa
     4141 agataagaag ttacatcacc ttacgtctaa acatcgcgta cgccgctgac cctgtcgtgg
     4201 ccgttatcac cagtagcggc cacaaactat tccacacaat atccagactc gcatccttca
     4261 aataaatctg cttggtaatg tccgtaaagt ggcgaatagg gttaatccac gtcaggtttt
     4321 gcagccatac cggcatgttt tccaccggag aaacgtaacc ggaaaggaga atggcgggca
     4381 tcataaagac aaacacgccg ataaacgcct gctgttgtgt tgaacagagt gatgaaatca
     4441 acagaccgaa tcccaccagc gataaaccat aaatcaccat cgtaaagtag aacagcgcca
     4501 gcgatccggc gaaggggatt tgatacgccc agataccaat cgccagcaca atggtggcct
     4561 ggaaggtggc gacaattaac gccggtacgg ctttgccgat gaagatctgc caggtggtga
     4621 gcggcgaaac cagtagctga tcgagcgtac cttgttcacg ttcgcgggcg acggaaagtg
     4681 aagtgacgat cattacgccg atagtggtga tcatggcgat cagtgacggc accacaaacc
     4741 atttgtagtc gagattcggg ttataccagt tgcgtaccac cagctcgctg ttgttaggtt
     4801 tcggttttcc ttccagcagc tcctgctgat aatttttgac gatctgttgc aggtagttgg
     4861 cggcaatttg cgcactgttg gagttacgcc cgtcgaggat caactgcaaa ggcgcggtct
     4921 ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg caccagtagt aacgcctttt
     4981 gtgtgtcgat ggttgggcgg atctcctgtg ggcttttcag cagcagcaca tgagtaaagg
     5041 cgctggcgcg ggcaaaacgt tgggtcagct ccaccgaatg ctcgccgtta tcttcatcgt
     5101 agatggcgat ggtggcgtta gtcacttcca gcgtggcggc gaacgggaac aggatcacct
     5161 gaattagcac gggtaaaatc agaatcgcgc gggtttgcgg ttcgcgcagc aacgactgca
     5221 actctttgcg gattaacgtc cataagcgat gaaacatgct cttctcccta atccagccga
     5281 cgtttggttt tcagccacgt caggccgata aacatcaccg ccgaagcgat caaaaacagc
     5341 acgtttacca ccagcaccac tggaatattc ccggcgagga acaggctttg cagggtgctg
     5401 acgaaataac gagcgggaat aatgtacgtc accgcgcgga tcaccgcggg catactgtcg
     5461 atctgaaaaa taaagccgga aagcataatc gacggcagaa aagcggcgtt cagggcgacc
     5521 tgagcggcat tgaactggtt gcgggtaatc gtggaaatca gcagccccat ccccagggta
     5581 ctgagtaaaa acaggctgga gataaaaaac agaatcagca gcgacccgcg atacggcacg
     5641 ccgagaataa acactgacac cagcatacac agcaacatcg ccagcatccc gagaaagtaa
     5701 taagggatca gcttacacag cagcagttcc gtgcgggtaa tctccgtaga gagcagagcc
     5761 tccatggtgc cgcgttccca ttctcgcgcc accaccagcg aggtgagaat cgcgccgatg
     5821 accgtcatga taatggtcac cgcaccgggg ataatgaagt gctggctaat cgccgccggg
     5881 ttaaaccagt agcgggtttg tacatcaata agcggttcaa aagtctgccc gttgtcctcc
     5941 gctcgctgca tttgccagat ctgccagatc ccttcgacat acccctgtac aaagttagcg
     6001 gtattcggct cactgccgtc ggtgatcacc tgaatcggtg cggtggcgtt ggcgcgctcc
     6061 atctgttccg caaaatccac cggaataacc accagaccgc gaattttccc cgcctgcatt
     6121 ttggcgatca gttcctgacg gttatcgctg atggtggcgt cgatgtaggg cgaaccggtc
     6181 atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt ccagtaaaat cccgacccgc
     6241 agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa acagcagtag cagcgggatc
     6301 actaccgcaa tcagccagct actcggatcg cgaacgatct gccgcgtctc tttaacgcac
     6361 agcgcccgta cgcgacgcca ggacaggatc gggttactca ttgctatgct ccttatccca
     6421 gtcgtggatc aactgaataa aggcttgctc catggtggga tcgggttgct cgtcgttggc
     6481 cgactgcgct ttcaaatcat ccggcgtgcc gctggcaatt aatttcccac ggtacaccag
     6541 gccgatgcgg tcgcaatatt ctgcttcatc cataaagtga gtggtgacca tcaccgtgac
     6601 gcctttctct accatgctgt tgatatgcag ccaaaattcg cggcgggtga ggggatcaac
     6661 gccggacgtt ggttcgtcga gaaacagaat gtccggttca tgcatcagcg aacaggccag
     6721 cgccagccgc tgtttaaaac ctaatggcag ttcatcggtc gcgtgggagg cgatactttt
     6781 caggccaaat gcctcgctca tacgggagat tttttcgttc tgcgcccgac cgcgtaagcc
     6841 atacacacca gagaaaaagc gtaaattctg ttcgaccgtc aggttgccgt agagcgaaaa
     6901 tttttgcgcc atatagccga gatgctggcg cgctttaccg gaactctctt tcagatccat
     6961 ccccagcacc agcgcctggc cggaagtcgg caccagcaaa ccgcacatca tcttaaaggt
     7021 ggtcgattta cccgcgccgt ttggccccag caaaccaaaa atctccccac gtttaacggc
     7081 aaagttgacg tgatcggtgg cggcaaaatc accaaatttc ttggtcagtt ctttcgcttc
     7141 gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat attgcaccca gcggcgattc
     7201 tgaggttccg gcaccaccca gcaaatcaat aaacgcatct tcaaaacgcg gcgtagtttc
     7261 gttgatgttt atttccggca tcccgtcggc atggcgaata tcgtctggtg tggcctcttt
     7321 tttgaggatc agacgtaccg atttcccctg aatcatgccg tcgctgacct gcggcagttt
     7381 caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga ctggtcatca gaaagctgcg
     7441 tccggccatg gtttgtgtca gggcttttgg ttctccctga tacagcaact cgccttcgtt
     7501 catcagtaac acgtcacggc actgctcggc ttcgtcgaga tacgaggtac tccagaggat
     7561 taacatccct tcgcccgcca gctcatgcac catctgccac agttcgcgcc gtgagatagg
     7621 gtcaacgccg acgccgggtt catcgagcag caacactttc ggttcgccca ccagggtaca
     7681 ggccaggccg agtttttgtt tcatcccacc ggagagcttg cccgccaggc gtccggtaaa
     7741 cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt tgcttacgtg cctcgccggt
     7801 gacgctgcgc aaatccgcgt acagattgag gttctccatc accgtgagat cttcatacag
     7861 accaaatttc tgcggcatat aaccgagcac ggcgtgcagc gcgccgtcgt ttttgatcgg
     7921 atcaaagcca atcaccgtgg cactgccgct gtcgggtttc agtaatcccg ccaacatccg
     7981 catcagcgtg gttttacctg caccgtccgg ccccaccaac cccgtcacat aaccggcgtg
     8041 aatggtacaa tcgagcggcg cgacggcggg cttgtccatg cccggaaagc gtttttccag
     8101 gccgttcagc gtgataacgg catcattcat gtcctgcctc gtcaccgaat tgtaccgtca
     8161 ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac cacaatacgc aggcgataga
     8221 cgaggtcggt acgcagatcc ggcgtttcga cggttttcgg ggtaaattca gcagtcggcg
     8281 aaacgaaacc aatctgcccg tgatacggct tgtccgggcg accatcggta taaagcagca
     8341 ctttgcgccc cggctgggcc tggtcaagat tacgttcatc aacataagcg cgcacccaca
     8401 ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc attgaggacc gtgcctggct
     8461 ccaccgcgcg cgttaacagc gtgccatcag acggggctat caacgttgag tcctgtaaat
     8521 tcaactccgc ctgcgccagt tgcgcctgcg cctgttcgag gctggctttc gcctgagcga
     8581 tgtcctgttc acggttaccg gaacggtact gacgcaattt atcctgtgct gatttcagcg
     8641 ttgcctgcgc ctggtcgcgc gaggagcggg cattttccag gtcatttgcc gaaatagtgc
     8701 ggcttttcca caacccttgc tggcggttat agaagttctg cgcatagtca taggcggctt
     8761 gcgcctgttt caccgctgcg gcggcctgag cgatttcttc atcgcgatac ccggcaagca
     8821 tcaggtcata ctgcgcctgt gccaccgaaa cacccgcttt cgcctgcatc agggcaatct
     8881 catacggctt gtgatccagt tcgcccagca cctggcccgc tttgatagca tcaccttcgt
     8941 ccaccgccag cgattcaacg cgccccccaa cacggaaact aagatttacc gtacgaatat
     9001 ccacgttgcc atacagcgtc aggccgttat cctggcggct ttgataccac cagtagcctc
     9061 cggcaaccac ggcggcaagt accactaccg ccaatccgat cacgacaggt tttttcatca
     9121 ctacaaactc ctttgcgata atccttgcaa aatcaggtcg atatgacagg tcaccgtctg
     9181 gttgatcagt tcggtctttt cttcatcgaa cgcggtccag ccggtacgta acagaattgt
     9241 ttctttgcca agacggaacg ccagaatctc accaatcaac gcatgggtat gaaggatcat
     9301 gcgggtgtca ttggcgtcgc agccggtcca ggcggcaatc aggcgtgtca ggtggctgtg
     9361 tagcggacta atcacctgtt cgtgcaccag gtggtaggct gccgtgggag agagctgctc
     9421 acgggagata aacttgctga ggttgacggt gtcatcctgg gtgagcagct taatcatgtt
     9481 cctgcaggcg cgaaggatca gttcacggat ggcagcccga tcaggctgtg gttgcgcgaa
     9541 caagcgttcg gcttcctcgg catgcggacg gaactgctcg ccaataaaat cggcaatcca
     9601 ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag tagtaggtga tggcagcgat
     9661 attctgcccg gcctgggcgg ctatctcgcg agtggtggcg ttcattccat attcaccaaa
     9721 ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt tcacccttga ttgtcatggc
     9781 aggattattc atcgcacagt ccattcttaa tcaaatgatt gattaagatt atgactccat
     9841 aggggagttg tccagtatgg ctaagaattt tagcaacgcc agtcacaggg ataatttatg
     9901 cgctgcgtca caaaaactgc tacactccgc tccctcatga cattgtggtt tttgtcattt
     9961 tccttttcag tatctccctg aaaactacac cggtaacggt cggggcggtt cggagtagtt
    10021 atgtctttcg attctttggg tttaagccct gatatcctgc gcgccgttgc cgagcagggt
    10081 taccgtgaac ccacccctat tcagcagcag gcgatccctg cggtgctgga aggccgcgac
    10141 ctgatggcta gcgcccagac cggcaccggc aaaacagcgg gctttacgct gccgctgttg
    10201 caacacctga tcactcgcca gccgcacgcc aaagggcgtc gtccggtacg tgcgctcatt
    10261 cttaccccga cccgtgaact ggcggcgcag attggcgaaa acgtccgtga ttacagcaaa
    10321 tacctgaaca ttcgttcgct ggtggtgttt ggtggtgtga gtattaaccc gcagatgatg
    10381 aaactgcgtg gcggcgttga tgtgctggtg gcaaccccgg gacgtttgct ggacctggaa
    10441 catcagaatg cagtgaagct ggatcaggtt gaaatcctcg tcctcgatga agctgaccgc
    10501 atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt taacaaaact acctgcgaag
    10561 cgccagaacc tgttattctc cgcgaccttc tctgacgata ttaaagccct ggcggaaaaa
    10621 ctgttgcaca acccgctgga aatcgaagtg gcacgccgca ataccgcgtc tgatcaggtg
    10681 acccagcacg ttcactttgt cgataagaaa cgcaaacgcg aattgctgtc gcacatgatt
    10741 gggaaaggga actggcagca ggtgctggtg tttacccgta ccaaacacgg cgctaaccat
    10801 ctggctgaac agctcaataa agatggcatc cgtagtgcgg cgatccacgg caataaatcg
    10861 caaggtgcgc gtactcgtgc gctggctgat tttaaatcgg gcgatattcg tgtactggtg
    10921 gcaactgaca tcgctgcgcg cggcctggat attgaagagc tgccgcacgt cgtcaactat
    10981 gaactgccaa acgtacctga agattatgtc caccgtatcg ggcgtaccgg tcgtgcggct
    11041 gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac acaaactgct gcgtgatatc
    11101 gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc cgggctatga gccggacccg
    11161 tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac gtggcggcgg cggtcgtggg
    11221 caaggtggtg gtcgcggtca acagcaacca cgccgtgggg aaggtggcgc aaaatctgca
    11281 agcgcgaaac ctgcagaaaa accgtctcgc cgcctcggcg atgccaaacc ggcaggcgaa
    11341 caacaacgtc gccgccgtcc gcgtaaacct gccgctgcgc agtaatcttt tatgccgggc
    11401 tatgcccggc atcaggctga tgaacaaacg caaaactgcc tgatgcgcta cgcttatcag
    11461 gcctacgtga actctgcaat atattgaatt tgcatgcttt tgtaggccgg ataaggcgtt
    11521 tacgccgcat ccggcatttc acaacaagca cttgtcagca atttgagaac acgggaaaaa
    11581 atttatctgt tttaccgccc ataagccacc gagacgttac ttctctatag ccaattgttc
    11641 gcgcaactcc attaaaaggt agcccagtcg attcttgccc ttaccatgac caccgtctcc
    11701 ccagtaagca tcgttttccg tatgctcaac cagttttgcg ggcgcggttg ccagcaagag
    11761 cgcacgcagt tctgcatgct gttcgaattt agcgcgaagt gcttttcgca tcacctgttc
    11821 tttgaccgac tcccagtttt tacgcagagg cttagaacga tcgcgcccca tgcgtgcggc
    11881 gaccataggg gaagaaaccc gacggatctc ttcgcggtat ttttcatcaa ggaatttttg
    11941 tgcctgaaaa tagtgttctg aggtgggcca ggttttcccg tcaaccttga tgggccaggc
    12001 ggcaaagtta gaaaaatcac cgtagtcatc gctggtgctg tagaaattta tgatggtgtc
    12061 ttgcatgacg tgttggatcc tttgtgctcg aacgggcatt aaaccgcatt atgttggtgg
    12121 ttattgcgag ccgctttcca gaaacagaaa aaccattacc cctgaaaacc gaaaaatgcc
    12181 acaatattgg ctgtttatac agtatttcag gttttctcat ggcattaacc gccgcgctta
    12241 aagcgcaaat tgccgcctgg tataaggcgc ttcaggaaca gatccccgac tttattcccc
    12301 gtgcgccgca gcggcagatg attgcggacg tcgccaaaac gctggccgga gaagaagggc
    12361 gacatctggc gattgaagcc cccaccggcg ttgggaaaac gctctcttat ttgattcccg
    12421 gcatcgccat tgcccgcgaa gagcaaaaaa cgctggtggt gagtaccgcc aacgtggcat
    12481 tgcaggatca gatttacagc aaagatttac cgctgctgaa aaagatcatt cccgatctta
    12541 aattcactgc cgcttttggg cgtgggcgct acgtttgtcc gcgtaatctg acggcgctcg
    12601 ccagtactga acccacgcaa caggatctgc tggcgtttct tgacgacgaa ctgacgccga
    12661 acaatcagga agagcaaaaa cgttgtgcga agctgaaggg cgatctcgat acttataaat
    12721 gggatggtct gcgcgatcat actgatatcg ctatagatga cgatctctgg cgtcgtttaa
    12781 gtaccgacaa agccagctgc ctcaaccgca attgttacta ctatcgtgaa tgcccgtttt
    12841 ttgtcgctcg tcgggagatt caggaagcgg aagtggtggt ggcaaaccat gcgctggtga
    12901 tggcggcgat ggaaagcgaa gcggtattgc ctgacccgaa aaatttactg ctggtgctgg
    12961 acgaaggcca tcacctgccg gatgtggcgc gggatgcgct tgagatgagc gccgaaatca
    13021 ccgcgccgtg gtatcggcta cagctggact tgttcacgaa actggtcgct acctgcatgg
    13081 agcagtttcg cccgaagacc atcccaccgc tggcgatccc tgaacgtttg aatgcgcatt
    13141 gtgaagagtt gtatgagctt atcgcctcgt taaacaacat tctcaatctc tacatgcctg
    13201 ccgggcagga ggcagagcac cgttttgcga tgggcgaact gccagatgaa gtgctggaga
    13261 tctgccagcg gctggcaaaa ctcaccgaga tgctgcgtgg cctggcggag ttatttctta
    13321 acgatttaag tgagaaaacc ggcagccatg acattgtacg tctgcatcgg ttgattttgc
    13381 agatgaaccg cgcgttgggg atgttcgagg cgcaaagcaa actctggcgg ctggcttcgc
    13441 tggcgcaatc ttccggtgca ccggtgacca aatgggcgac gcgggaagag cgcgaagggc
    13501 agctacacct ctggtttcac tgcgtgggaa tacgtgtcag cgatcagctg gaaaggctgc
    13561 tgtggcgcag tattccgcac attattgtca cctccgcaac cttgcgttcg ctgaacagtt
    13621 tttcgcgttt gcaggagatg agtggtctga aagagaaagc gggcgaccgt tttgtggcgc
    13681 tggattcccc ctttaaccac tgcgaacagg gcaaaattgt tattccccgg atgcgcgttg
    13741 agccttccat cgacaacgaa gagcagcata ttgccgaaat ggcggccttt ttccgtaagc
    13801 aggtggagag caaaaaacat ctcggtatgt tggtactgtt tgccagcgga cgggcgatgc
    13861 agcgctttct cgactatgtg acggatttac gtctgatgtt gctggttcag ggcgatcagc
    13921 cgcgttaccg tttagttgaa ctgcaccgca aacgcgtcgc caacggtgag cgcagcgtgc
    13981 tggtgggctt acagtcattt gccgaagggc ttgatttgaa aggtgatctg ctcagccagg
    14041 tgcatatcca caaaatcgct tttccgccca tcgacagccc ggtggtgatc accgaagggg
    14101 aatggctgaa aagcctcaac cgctatccgt ttgaggtgca aagcctgccg agcgcctcgt
    14161 ttaacctgat tcagcaggtt gggcgactga ttcgaagcca cggttgctgg ggcgaagtgg
    14221 ttatctacga caaacgcttg ctgaccaaaa attatggcaa gcgactactg gatgcattac
    14281 cggtatttcc gatagagcaa ccggaagtcc ctgaaggtat agttaaaaag aaagaaaaaa
    14341 cgaaatcccc acgccgtcgg cggcgttaat gatgtgagtc aggtaaggag tcgtaaatgg
    14401 actatcgcaa aatcattaaa gagatcgggc gcgggaaaaa ccacgcgcgc gatttagacc
    14461 gggatactgc gcgcggtctg tatgctcata tgctcaatgg tgaagtccct gaccttgagt
    14521 tgggcggcgt attgattgcg ctgcgtatca aaggagaagg ggaagcagag atgctcggct
    14581 tttacgaagc catgcaaaat cacaccatca agctgacgcc gccagcaggc aagccgatgc
    14641 cgattgtcat ccccagttac aacggcgcgc gtaaacaggc caacctgacg ccgttgttgg
    14701 cgattctcct gcacaaactc ggttttcctg tggtggttca cggggttagc gaagatccaa
    14761 cccgcgtgct gactgaaacc atttttgaat tgatgggtat tacgccaacg ttgcacggcg
    14821 ggcaggcgca ggcgaagctc gacgaacatc aaccggtgtt tatgccagtc ggcgcgtttt
    14881 gcccgccgct ggaaaaacaa ctggcgatgc gctggcggat gggcgtgcgt aacagtgcac
    14941 ataccctggc gaaactggcg acgccatttg ccgaaggtga ggcgctgcgt ctttccagcg
    15001 tttcgcatcc ggaatacatt ggacgcgtcg cgaagttctt tagcgatatc ggcgggcggg
    15061 cgctactaat gcatggtact gaaggtgaag tgtatgctaa tccgcagcgc tgcccgcaga
    15121 tcaatctcat tgaccgtgaa gggatgcggg tgctgtatga aaaacaggac actgctggta
    15181 gcgagttact gccacaagca aaagatccgg aaaccacggc gcagtggatt gagcgttgcc
    15241 ttgctggcag cgaaccgatt cccgaatcgc tgaaaatcca gatggcttgc tgcctggtgg
    15301 ctacgggtga agcggcaact atcagcgacg gcctggcgcg cgttaatcag gcattttaat
    15361 tcttttctca gccggatgag ccatgtcatc cggcttttac cccaccgcaa tatgaaattc
    15421 ctgcatcttt attgaccttc ccacgcccgg cgtgcagcat aaaaatacaa caaacacata
    15481 acataaacag gagttaacca tggaaagtgg tcatcgcttt gatgctcaga cgctgcacag
    15541 ttttattcag gctgtatttc gtcagatggg tagcgaggaa caagaagcga aattagttgc
    15601 cgatcattta atcgcggcaa acctggcagg gcatgattca catggtattg gcatgatccc
    15661 aagctatgta cgctcctgga gtcaggggca cctgcaaatt aaccatcatg ccaaaaccgt
    15721 taaagaggcg ggggcggcgg tcacgctcga tggcgatcgc gcatttggtc aggtcgcggc
    15781 acatgaagcg atggcgctgg ggattgagaa agcgcatcag cacggtattg ccgccgtggc
    15841 gctacataac tcgcatcata tcggccgtat cggttactgg gcggagcagt gtgcagcggc
    15901 ggggtttgtc tctatccact ttgttagcgt ggtcggtatt ccaatggtcg cgccgttcca
    15961 cggtcgcgac agccgctttg gcaccaatcc gttctgtgtg gttttccctc gtaaagataa
    16021 tttcccgctg ttgcttgatt acgccaccag cgccattgca tttggcaaaa cccgcgtcgc
    16081 ctggcataaa ggcgtccccg tgccgccagg ttgcctgatt gacgttaacg gcgtgccgac
    16141 gaccaatccg gcggtaatgc aggagtcgcc gttgggttcg ctgttgacct ttgccgaaca
    16201 taaaggctac gcccttgcag cgatgtgtga aattcttggc ggggcgcttt ccggcggtaa
    16261 aacgacgcat caggaaacgt tacaaaccag tcccgatgcc attcttaact gcatgaccac
    16321 tatcatcatc aacccggaac tcttcggcgc gccggattgt aacgcgcaga ccgaagcctt
    16381 tgccgagtgg gtgaaagcct cgccgcatga tgatgataag ccgattttgc taccgggcga
    16441 gtgggaagtg aacacgcgtc gcgaacggca gaagcagggg attccactgg atgcgggaag
    16501 ctggcaggcc atttgtgatg cagcgcggca gattggtatg ccggaagaga cgttgcaggc
    16561 tttctgtcag cagttagcca gctaaaaaaa agcccgtcca gtggcggacg ggcaaacaag
    16621 ggtaacatag gatcaatgag ggttagagca tatgcgtctg tcggcaaaca gacagggaaa
    16681 tacttgtgct ggacgtagcg taaacgcctg atccgtccaa cggttcggat tttgtaggcc
    16741 tgataagacg cggtagcgtc gcatcaggca tctgttgccg gatgcggcgt aaacgcctta
    16801 tccgtcctac gaattactta taaattaccg cagtaccgct taatttattg ttgttggtgg
    16861 cggaagtgat gctgtatcca ctggcaccgg ctgccgcggc tttctcagcc agtttcgctt
    16921 ccagggcatc gagggtggat gcgccatcgg cagaaaccac gccgatttta ttcatgttct
    16981 gtgcctggga tgccgtcacc ggttccgccg cgaacacgcc aaatgacaga gttgaaagag
    17041 ccatagcagc aacaacagta ttgatagttt tcataattaa tctctcgcag gtgatttttg
    17101 tataagtgac gttgtttcgt cgatgtgatg agtatcacgt ttttttacga gagataaaat
    17161 cgaagagaat tgaccgcctt gttcaaataa attgattgat atctaaccta ctgataaacc
    17221 atttatttgt tgtacttatg aacttgtttt ataccgcgtg gcagtcacag cagcgttaaa
    17281 ggcacggaat gccacgcaaa atacaaatta tactcgctat gcatctcgcc agttgtagtt
    17341 aacgtaactg gctgtctttc gaacctctgc gattatatcc tgtataagct ggtttttgta
    17401 aatctttctc ctgctgacaa tgaatacata agcgcacgcc aggaatggct tcccgacggg
    17461 cctgcgggat gggggcaccg cactcttcac attcatccag gctttcgccg cgcggaattt
    17521 caccccgagc gcgggcaatc gcatcttcaa ttgtactgtt gatctgttcg ttgacggcgt
    17581 catcgttagc ccaaccggat gccatatcga cctccccata tcaatacttg tacagttaag
    17641 tgtagctaat ccagggacga actcgggcag ttcaagcatc agatctccga ccattcccgc
    17701 agcagattat gataaagatt aagcagcgac aggatctctt cactttcgcc gtagcggctt
    17761 ttcagcgact gaatattgtt gtccagttca aacagcatgg cgcgcttttt atcatcgcgg
    17821 atcatcgact ggatccacat aaatgatgcc actcgtacgc cgcgggttac gggtgtcacg
    17881 caatgcaggc tgctggaggg atacaacacg agatcgcctg ccgggagttt tacccgatgt
    17941 tgtccgaagg tgtcattaac gaccagttcg ccgccgtcgt agctttgtgg atcgcttaaa
    18001 aacagcgtgg cagaaaggtc agtacgcatc cagccgtttt gtggatggct gcgtactgcg
    18061 ccatccacat ggaaaccata ggtttcattg ttctgatagc gattaaacag cggcgtggaa
    18121 agggtacgcg gcaaggccgc ggcaaagaat aaagcatgtt ggttaaccgc gttcagcacc
    18181 tcattttgca gggcggcgta taacgtgctg cgggtgtcga cctgttgatt gttcttaact
    18241 tgcgcacctt gtgcgccggt ggtgacgcgt ccatccaccc attcggcttg ttccagttgt
    18301 tcgcgaaaac gagcgacgtc ctgtggcgat aacacgccgg gaatgtggta catcatacaa
    18361 tttctccaaa aagtggggcc tgcgccccac atctgattca gaaatgcata ttggctgtga
    18421 gcaagaaggt tcttggctcg cccgggtgat aacggtagcc gctcttgttg attgaggcga
    18481 cgtaatcggt atcaaacagg ttgtaaacgt ttagctggaa gtcgagattg cgattaactc
    18541 gataccccag tttggcatcg gcgacccagt aaccttcggt aaacgctggc gttcccaccg
    18601 cgccgtctga acctttatgc atactgccga tatagcgtgc gcccgcgcca acagagatat
    18661 cgtcggttgc ctgatattgg ctccataagg tgaaggcgtg ctccggggta tacggcagca
    18721 atgaggaacc atcctgggca acatctttgc cgtttttgat ggttgctttt tgctgggtat
    18781 agccgccaat cacctgccac gcgggagtga tattcccggc cacggatatc tcatagcctt
    18841 cgacgcgttt cttaccgtat tgcgagtaag ttccgtcatc attttgctca acttcatttt
    18901 cgatatcagt gcggaacagc gcggcggtga gcaacagacg tttatccaga acctgccatt
    18961 tggtgccaat ctcgctggtg ttggcttttt gcggtttaaa atcggtgcgg ttggcactgt
    19021 taccgctgcc agactgcgca agggcgaagt tgttgccgcc cggaggctgc tgggaaacgg
    19081 catagttaat atagacattg ccgttttccg tcaggtgata cagcgccccg gctttccagt
    19141 tcatcagatt gcccgacttg gcggtgtcga cggtggtgac cggagaacct tttgccacac
    19201 cagttgggca ggtgatggca ccgcgtccgc tgccgccgca ggcggtggca ctgtcatatt
    19261 cagtatgata attatccaga cggatcccgc cgttcagctc aaaatcacgg gtgatttgca
    19321 gcgtatcaaa ggcgtaaatt gcgaaggtat ccgtctgacc attggcgttt gcgccgttgc
    19381 gcgtcaggcc gccgggatga atgctgctgt caggatgata aatatttacc gcgggtaacg
    19441 tcaccggatt aacgccgtag ttagtctgcg tttcacgggt aaattccacg ccggtactga
    19501 catcatgacc gatagaaccg gtatagaacg tcgaggtcag gttggtctgg ttggtgagaa
    19561 ttttattact cacatctttg gtattcgccg tgcgtgacca ggtccagcta ttcacatcgc
    19621 tggtgggctg agtaatattc gacgccccgc ccataatcgc cgtcatcagg taatcctgct
    19681 ttacgcgcga ccaacgggta gtattgcgaa tggtggtgtt atcgttgatg tcgtgctcaa
    19741 aacgcatggt ggcggtgtcg gtggtcgaat cgtcgtaatc ggaatccgtg ccgtaaaagt
    19801 tatgagtatc aacttttccg gaatgattca gggccgccgt tcccgcagat ggggcagaat
    19861 agcccggcaa accgatggtc ggaatgccgc cgtctggcgt gttgtgctgg gtgacatgca
    19921 gataattaag atacaaacga ttcgctgtac caaggccaaa agcgacagaa ggggcgacgc
    19981 cgtaacgctc atttttgact ttgtcgcgtc cggcatcgtg cgttttttcg cccattacat
    20041 tcaggcgcac cgcggtggta tcaccaatga cctgattgac gtccagcgtg ccgcggcgga
    20101 accaggcgct gccaatactg gcggaggcgt caatgccgga atcattgcgc ggctgcttgc
    20161 tgatcatatt gatcgagcct gtcggtgcgc tgcgcccgta gtcggtgccg gacggccctt
    20221 taatcacttc gacctgctcg gtattgaagg tgtcgcgcga gacgctgccg atatcgcgaa
    20281 tgccatcaat ataaatactg ttagaggtat cggcaccacg catataaatg gcgtcgccag
    20341 tggtggagtt accgttctca cccgcaaaaa acgcacccac gccggggacg tttttgagcg
    20401 cgtcggtaag gttggttgcg ccctgatctt taatcacttg ttcagaaatt accgtcatcg
    20461 tgcgggtagt atccgctacc ggacgcgaga atttcggatc ggcagattgt tgtggcgcat
    20521 aaagcgaagg cgttgatgct tcgacaacca gcgtgtcatc cgcgttagtt tgcccttcgg
    20581 cggcgagtgc ctgagccaca ggcgtgatgc caatacaaag accggcaaag aacgtgagcg
    20641 aatgaaattg tctggcaggg aaattgcgat tgttttccat tttgcaggtg actttttctt
    20701 atatgttgaa attttcactg ccacgagaaa gcatctctgc ggatgcgatg ccactgttaa
    20761 tcccgtgata gtgttcgtgc aagtgataat aattttgata agcattatca cttggagcga
    20821 ttttctatca cccattgcgc gaaaaagaaa tacatttatt tacattgagt tcatatttga
    20881 tgttttttga ttacaaaaca gacgaaaact gcatccagag gcgaatgcag ttacgttggt
    20941 ggggttattt gtagatggtt gcagtaccga acatctggtc atttccgcct gcggaattaa
    21001 tgacataacc tttcgcgcct tgttcgcgcg ctttttctgc cagtttatcc tccagatcgc
    21061 ttaggtttga tgcgccagtt gccgaaacgg taccggcggg ccgtagctga ccgctgtcca
    21121 ggttactcat cggttgcgcg gcataagccg tgagagagat tccgctcagg acggtggcaa
    21181 tcagtagtgt gaggcacttt ttcataatga catcctcata aatacaacgg gtagtgcctg
    21241 ataagtgtag gtcgcctgcg cgatgcaaat gacgcaaaaa attgacagag atgtgcttat
    21301 tttttgaacg atatattttt acaaaataag acaaatcaat gacatgcata atgctgattt
    21361 ttatgatgaa ttgccataaa ccatagccat gttaaggttt tggggaatga taaacgcagc
    21421 cagatattta ccctgtcgcg ttatgcgggt atcttacgcc gctgcttaaa ggagaatgct
    21481 atgtccgccc agaaaccggg gttgcatccg cgcaaccgtc atcacagccg ctacgatctc
    21541 gccacgcttt gtcaggtcaa tcctgaactc aggcaattcc tcacgcttac acccgccggg
    21601 gagcaaagcg tagactttgc caatccgctg gcggtgaagg cgctcaataa ggcgttgctg
    21661 gcccattttt acgccgtagc gaactgggat atccccgacg gttttctctg cccaccagta
    21721 ccgggccggg cggattatat tcatcacctt gccgatttac tggcagaagc aagcggaaca
    21781 attccagcga atgccagcat tctggatatc ggcgttggtg cgaactgtat ttatccgctg
    21841 attggcgtac atgaatatgg ctggcgtttt accggtagtg aaaccagcag ccaggcgtta
    21901 agcagtgcgc aggcgattat cagttctaat ccggggctta accgcgccat tcgtctgcgt
    21961 cggcaaaaag agagtggggc gatttttaac ggcatcatcc ataaaaacga gcaatacgac
    22021 gcgaccttgt gtaacccgcc attccacgat tccgccgctg cggcacgggc aggtagtgag
    22081 cgtaaacgcc gtaacctggg gctgaacaaa gacgatgcac tgaactttgg cggccagcaa
    22141 caggagttgt ggtgtgaagg cggtgaagtc acctttatca aaaagatgat tgaagagagc
    22201 aaaggcttcg cgaagcaggt gatgtggttt acatcactgg tatctcgtgg tgaaaactta
    22261 ccgccgttgt atcgtgccct gacggacgtg ggcgcggtga aggtggttaa aaaagagatg
    22321 gcccaggggc aaaagcagag tcgctttatt gcctggacct ttatgaacga cgagcagcgc
    22381 cgccgttttg tcaatcgcca gcgttaaagc gttggttccc ccggcggtaa cggttcagcc
    22441 ggggtcgcgc ccggagcagg cagcacctga taagtctgca ctggcgcgcg aacgcccgcc
    22501 aggtcgaaat gttttttcac ctggctgtcg agggcaaagc gtaccgtcca ctgtttgagt
    22561 ggcagcgtgg tgaacgaaac acgcagtgta aacgcggtat tgcttaagcc gacaatcccg
    22621 gcaaaattcg gttcaccaat aatcagcccg cgaatttctt cgttttccat taattccgct
    22681 accgcatctt tcagtgcctg attggcttta tcagcatctt catggcgatc aacatcataa
    22741 tttgccacca ccgaaccaat gccgcggaca aagttagcaa aggtggttat cgaagaccac
    22801 ggaatgatgt gatacgcccc ggtatcctgg cgtacgccca cggagcgaat cgacatccgt
    22861 tccactgtgc cggtcaacgg cccgatagtc accaaatctc cagtgttcat gccgttttca
    22921 aactgaataa ataccccggt gataatatct ttcaccagcg tttgcgaacc aaacgagata
    22981 gccagaccta atgccccggc acctgccagc aatggcgcga tattgacgcc gatttccgac
    23041 aacacaatca tgatggtgat ggtactgata atcaccgcca gcgcgttacg aaacagcgta
    23101 agcagggtac gcgtgcgggc gctgggtagc gggcggccat gaatatccga agccagccgg
    23161 ttttcgatca aactggcgag caccgtccag ccaaccgccg agaagaaaag aatgagtgcg
    23221 atacggatca ggatatctac ggttttctgc cccgcgccgt tttgcagcca gttccagaaa
    23281 tcgaacaatc cccatgcgct caacagcaac attaccgcca cgcagactgt cagaatacgc
    23341 gccgttttca gcgccgccga cagccagcca ttcaaccgtt tttgcagctc cggatagtta
    23401 cgctgagtat gtggcgagag agtgatggtt ttcgccagcc agcgggaaaa cataccggaa
    23461 acaaacgctg cgataccaat aatcgccagg ctgcgcaccg ttgcacccat cataaatttc
    23521 aggctgttgc ccggatcgaa caacgaaaag aaaaacagca cgataaaata ggcgcttgcc
    23581 agccagtgcc acaccagcgc aaaggcgcgg ataaacaggc tgaaaaaggc cagcgaatgc
    23641 tccgcgaagt tgaacaaatg ctgggtaatc tcttttttat tacgaaagat caggtacaac
    23701 gcccagacgg tcatgcacag cataatgatg acgttcgcca gcgcacctat ctgtacattc
    23761 acctgattag agataatcgg cacggccaca atcaggccat aacctatcaa actgcttaac
    23821 cagctcaggc gacgactcca gtaacgggcg ctctcgtcct gaatcgtgaa cgggcgcagc
    23881 tccgccacgt ttgggcaaaa aatcaggcgt agtacggctt tgaaaaattc aatgagggca
    23941 aaggcgttga gaaacaaact ttgttggaaa gcgatggtgc gactgcctgc attcaggttg
    24001 tcgcttaata cctggccgac aaataatgtc agggccagta acagcaggtc gataataaac
    24061 gccccgataa tcatcgccgg aagctgcaac cagttgctgc gctcacgatt tttttgccgc
    24121 gcccactgac ccattttgcg atacagcggc agtgcgcaca ggcgaatcag ccagtaaaaa
    24181 ccaaacacta ataccgctaa cattgaaaaa tgggtcagcg cattgctgaa ggtttgtgga
    24241 ttaaacggct tatgcgggga gccggtgata ttgcgataaa gttgcccgaa gcgggcggaa
    24301 agggcttcac cataatggcg gctgacttcg gtgacctttt gcagcacggt ttgctcttcg
    24361 accagcgtcg gcggcactat ttttggtacc ggttcagcag ggggcgtagc ggcaacggtg
    24421 cgcaactggt cgatcaactc tttacgcgag gtgtcattat ccagcacatc cgccagtgcg
    24481 ccataggccg cttttttttg ttcgatatcc ggttccgggg ccggttcagt cgttgagtcc
    24541 gttgtcgttg tggttgtaac gccgggtatg gataccgcgt gggcaggtgc gcccagcagg
    24601 cagaagagga tgaacaggat ccaccgcatg actcctccag tgagaaaata gagcaaaaag
    24661 ataagtatag atgctggagg aggggcgatt ttaaatgaga ggaatctggt gtgcctccct
    24721 ttcgggtgaa agggaggaag ggatattaag caggttgctg acaacgtgcg ggcttttttg
    24781 ccggatgcgg cgtgaacgcc ttatccgtcc tacaagttca tgcaatttta ataagttgcc
    24841 agatatcctg taggcctgat aagcgtagcg catcaggcaa tgtgtcttat taagagacgt
    24901 gctgcaaaaa ttcctgcaag cgctggctcg gcgggttctt gatcaacacc tgcggattgc
    24961 catcttccgc aatccggcct ttgtcgataa agatcagccg cgaagctact ttctcggcaa
    25021 aaccgatttc gtgggtcacg atcaccatcg tcatcccttc ttcagccaga tcctgcataa
    25081 ccttcagcac ttcatggcgc agttccgggt caagagcgga agtcggttca tcaaacagca
    25141 tcattttcgg cttcaccgcc agcgcgcggg caatcgccac acgctgctgt tgaccaccag
    25201 aaagttcgga agggtagtga tgtgcacgtt ctgccagacc gactttcgcc agcagctcac
    25261 gtgccagttt ttccgcctct tctttgttcg cgccacgcac gcgtagcggg ccaaacatga
    25321 cgttttccag cgccgtcaga tgcgggaaga ggtaaaactg ctggaacacc atacctgctt
    25381 cctggcgaat caggcgctcg tcaactttcg gatcgttaac cttcaggcca tcgacaatca
    25441 gatcgccgga ggtgatttct tccagtttgt tgatgcagcg cagcagggtc gatttaccgg
    25501 aaccggacgg cccgataatc accacgactt cgccctgggc aatgttcaaa tcgatattgt
    25561 gcagcacctg ggttgggcca aagtgcttgg agacgttttt aaattcaatc acaggatttt
    25621 catccttctt tccagacgac gcagaataaa gctcagcacc agggtaataa tcagatagaa
    25681 caccgccacg gcgctccaga tctcaagggc gcggaagtta ccggcaataa tttcttgccc
    25741 ctgacgggtc agttccgcca cgccgatcac aataaacagc gaggtgtctt taatgctgat
    25801 gatccactgg ttacccagcg gcggcagcat acgacgcagt gccagcggta aaatgacgta
    25861 gcgaatggtt tcccaacgtg aaagaccgag cgccagtcct gcttcacgaa aacctttgtg
    25921 gatagacagc accgcaccac gcgtgatttc cgcaatatac gcgccggagt tgatcatgat
    25981 ggtgaccacc gccgcagtaa atgggtcgat gcgtaagtcg ttaaacgcca tcggcagggc
    26041 gaaataaata aacatcacct ggacgacgat aggtgtgccg cggatcactt caataaagac
    26101 cagcgcgacg tggttggcta tccaacctcc gaaggtgcgt gcaaaacctg ccagcaatcc
    26161 gattaccaga ccgcctgcca gaccgaggac cgaaatccac agggtcattt tggcaccttc
    26221 aatcagaagc ggaatggcag gccagatggc actccagtca aactgcatat gttgttcctg
    26281 ttaccgtggt gtaaataatc aaattcaggg taattaatgg ccccggagcg ggtttgcgcc
    26341 cctcacccta atcctctccc cataggggag agggaactgc cagtgcgttt tacaggtgta
    26401 gcgttattat ttcggttcag taccgaacca ttttttgtag atttcgttgt aagttccgtt
    26461 ctcgcgcagg gttttcaacg cgccgttgac tttgtcacgc agctcgtcgc tacctttcgg
    26521 gaacgcaata ccgtattgct gcgcttccag agagtcacct accgctttga actgaccgtt
    26581 accggcggtt ttgatgaagt acagaatgtt tggcgtatcg tgcagaacgg cgtctgcgcg
    26641 gttggtgccc agttccatat aggcgttatc gatgttcggg aactgacgca gatctttagt
    26701 tttgatgttt gctttcgcgt aatcaacgga gccagtaccg ctcttcacag caaccacttt
    26761 cccgtcgaga tctttcacgc ttttcacatc gttattgtta gctttcacca tcactaacag
    26821 gccgcttttg tagtagccgt cagagaaatc gatcgctttt ttacgctcgt cggtgatggt
    26881 aatgcccgcc agcgccagat cgacgttttt ggtttgcagt gccggaatga tcccactgaa
    26941 atccatcggc ttcagttcgt aatccagctt cagctcttta gcgatggcag cccacagatc
    27001 aacgtcaaag cccacatatt tatcgccctg tttaaattca aacggaacga aggcggtatc
    27061 cgtcgcgaca actaattttt tatccgcggc atgagaagaa accgcaaaag ccagggtcag
    27121 tgcagccagt gaaactttta atacagactt catagcattt ccttttttct atccacggga
    27181 cgatcccctg cgtgacattc acatatatga aaaaatcgtg ccagttttgc aactccttga
    27241 tttttcgaga tgcggcaaaa acgtgatgca cgatttatag ggcaataccc tgaagatgca
    27301 ccattctggg gcaccaatct ggtgcgctaa aattgtgcac tcaacacagt gattatttag
    27361 cgtaaaacct gatgaaaaaa caatctttat gtaacgattg tgtgatgatg tggatacaaa
    27421 aaatttactt aatcagctgg agatagcaga tggatgcact aaataagtgc gttgaggtgg
    27481 gctgcaaaac aaaacggcct cctgtcagga agccgctttt atcgggtact aaagttctgc
    27541 accatcagcg atggatttat tcgatgttag actcgataaa ccacaggaat ttatccaggt
    27601 cgcgagacgc ggcggtcagg atatctgcgg tgtcgtcatc tttcgcttcg ccaatcgctt
    27661 tgcgtacgtc attagcgacg attgcgtaac ggtcagccag ttctttcagg tgatcctgaa
    27721 cgttgtggat gtccagcggg taacttttca gcggggtttt gctgttgata acttgagtgg
    27781 tccccagagc tacaccgccc agctgcactg cacgttctgc catggtatcc agatgatcga
    27841 tcagtgcggt gcggaagcca tccagcattt catgtacggc aatgaagtta gcgccgcgca
    27901 tgttccagtg cgcttgtttg gtaatcaaag aaagatcaat aaactggata acctggcgat
    27961 tcagcaactc tactgttgct tttttctcgc tgtcggagac atcgttgcgg gtataaagca
    28021 gattggtcgc ttttgattta actaatttag cggtactcat aatttcatat cctcttgatg
    28081 ttatgtccca gtaattaacg agactaagta tagcaccggc tatgtgttcc gctattctgg
    28141 ctgttcctat cacactaata gtggtaacaa gcgtgaaaaa c
//
LOCUS       DAJOSY010000058        27836 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJOSY010000058 DAJOSY010000000
VERSION     DAJOSY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.058"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            55..378
                     /gene="iraM"
                     /locus_tag="OHN27_002555"
     CDS             55..378
                     /gene="iraM"
                     /locus_tag="OHN27_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCQ2286969.1"
                     /translation="MKWIVIDTVIQPTCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYHIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(481..1731)
                     /gene="icd"
                     /locus_tag="OHN27_002556"
     CDS             complement(481..1731)
                     /gene="icd"
                     /locus_tag="OHN27_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCQ2286970.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1903..2556
                     /gene="rluE"
                     /locus_tag="OHN27_002557"
     CDS             1903..2556
                     /gene="rluE"
                     /locus_tag="OHN27_002557"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCQ2286971.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2566..3027
                     /gene="nudJ"
                     /locus_tag="OHN27_002558"
     CDS             2566..3027
                     /gene="nudJ"
                     /locus_tag="OHN27_002558"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCQ2286972.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3081..4187
                     /gene="mnmA"
                     /locus_tag="OHN27_002559"
     CDS             3081..4187
                     /gene="mnmA"
                     /locus_tag="OHN27_002559"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCQ2286973.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4223..4864
                     /gene="hflD"
                     /locus_tag="OHN27_002560"
     CDS             4223..4864
                     /gene="hflD"
                     /locus_tag="OHN27_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCQ2286974.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLDVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4868..6238
                     /gene="purB"
                     /locus_tag="OHN27_002561"
     CDS             4868..6238
                     /gene="purB"
                     /locus_tag="OHN27_002561"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCQ2286975.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6407..7078
                     /gene="phoP"
                     /locus_tag="OHN27_002562"
     CDS             6407..7078
                     /gene="phoP"
                     /locus_tag="OHN27_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCQ2286976.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHI
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7078..8538
                     /gene="phoQ"
                     /locus_tag="OHN27_002563"
     CDS             7078..8538
                     /gene="phoQ"
                     /locus_tag="OHN27_002563"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCQ2286977.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGDSMLGGARMEVIFGRQHSTPKDE"
     gene            8614..9735
                     /gene="roxA"
                     /locus_tag="OHN27_002564"
                     /gene_synonym="ycfD"
     CDS             8614..9735
                     /gene="roxA"
                     /locus_tag="OHN27_002564"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCQ2286978.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHHDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9784..11010)
                     /gene="pepT"
                     /locus_tag="OHN27_002565"
     CDS             complement(9784..11010)
                     /gene="pepT"
                     /locus_tag="OHN27_002565"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCQ2286979.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11260..12396
                     /gene="potA"
                     /locus_tag="OHN27_002566"
     CDS             11260..12396
                     /gene="potA"
                     /locus_tag="OHN27_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCQ2286980.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12380..13237
                     /gene="potB"
                     /locus_tag="OHN27_002567"
     CDS             12380..13237
                     /gene="potB"
                     /locus_tag="OHN27_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCQ2286981.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13234..14028
                     /gene="potC"
                     /locus_tag="OHN27_002568"
     CDS             13234..14028
                     /gene="potC"
                     /locus_tag="OHN27_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCQ2286982.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            14025..15071
                     /gene="potD"
                     /locus_tag="OHN27_002569"
     CDS             14025..15071
                     /gene="potD"
                     /locus_tag="OHN27_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCQ2286983.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            15129..15590
                     /locus_tag="OHN27_002570"
     CDS             15129..15590
                     /locus_tag="OHN27_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HCQ2286984.1"
                     /translation="MSQDSKVFFRIFLGIGLVLILISVVVFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKDGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKSRRKRKSRP"
     gene            15587..16375
                     /locus_tag="OHN27_002571"
     CDS             15587..16375
                     /locus_tag="OHN27_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCQ2286985.1"
                     /translation="MKKFIILLSLLILLPLTAASKPLIPIMKTLFTDVTGIVPDAEEI
                     AHKAELFRQQTGIALFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQST
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSRIFYYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16495..17334)
                     /gene="cobB"
                     /locus_tag="OHN27_002572"
     CDS             complement(16495..17334)
                     /gene="cobB"
                     /locus_tag="OHN27_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415638.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCQ2286986.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGLKAG
                     SIA"
     gene            complement(17350..18261)
                     /gene="nagK"
                     /locus_tag="OHN27_002573"
     CDS             complement(17350..18261)
                     /gene="nagK"
                     /locus_tag="OHN27_002573"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCQ2286987.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(18290..19534)
                     /gene="lolE"
                     /locus_tag="OHN27_002574"
     CDS             complement(18290..19534)
                     /gene="lolE"
                     /locus_tag="OHN27_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCQ2286988.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19534..20235)
                     /gene="lolD"
                     /locus_tag="OHN27_002575"
     CDS             complement(19534..20235)
                     /gene="lolD"
                     /locus_tag="OHN27_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCQ2286989.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(20228..21427)
                     /gene="lolC"
                     /locus_tag="OHN27_002576"
     CDS             complement(20228..21427)
                     /gene="lolC"
                     /locus_tag="OHN27_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCQ2286990.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVVGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21689..22762
                     /locus_tag="OHN27_002577"
                     /pseudo
     CDS             21689..22762
                     /locus_tag="OHN27_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            22890..26336
                     /gene="mfd"
                     /locus_tag="OHN27_002578"
     CDS             22890..26336
                     /gene="mfd"
                     /locus_tag="OHN27_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCQ2286991.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQTQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26480..27442
                     /gene="ldtC"
                     /locus_tag="OHN27_002579"
     CDS             26480..27442
                     /gene="ldtC"
                     /locus_tag="OHN27_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCQ2286992.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWQLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27525..27782)
                     /gene="bhsA"
                     /locus_tag="OHN27_002580"
     CDS             complement(27525..27782)
                     /gene="bhsA"
                     /locus_tag="OHN27_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCQ2286993.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6838 a         7182 c         6997 g         6819 t
ORIGIN      
        1 agaaataaac atcctaatga accatattaa ataccgtggg ataagacata acaaatgaag
       61 tggatagtaa ttgacacggt aattcaacct acatgtggta tatctttttc agccatatgg
      121 ggtaatatga aaatgattat ctggtatcaa tctactatat ttctccctcc tggcagtata
      181 tttacaccgg ttaagtctgg tattatcctt aaggataaag aatatcctat tactatttat
      241 cacatcgcac cattcaacaa ggatttatgg agtttactca aaagcagtca agagtgtcct
      301 ccaggagaaa gcaaaataac aaataaatgt ttacataata gttgcattat aaaaatatgc
      361 ccatatgggc tcaagtaatg ggcttcatat aaatatgctc gatatgctat taataatatc
      421 cacataaata aaacaacggg cgtgttatac gcccgtttca atatttaaca catggagaga
      481 ttacatgttt tcgatgatcg cttcaccaaa ctctgaacat ttcagcagct tagcgccttc
      541 catcagacgt tcgaagtcat aagttacggt cttggcattg attgcgcctt ccataccttt
      601 aacaatcagg tcagccgctt cagtccaacc catatggcgc agcatcatct cagcggagag
      661 gatgatagag cctggattta ctttgtcctg accggcatat ttcggcgcag taccgtgggt
      721 ggcttcaaac agggcgcatt cgtcaccgat gtttgcacca ggggcgatac cgataccgcc
      781 aacctgcgct gccagggcgt cagaaatgta gtcaccgttc aggttcatac aggcgataac
      841 atcatattca gccggacgca gcaggatctg ttgcaggaat gcatcagcga tcacgtcttt
      901 aatgacgatc tctttgccag tgttcgggtt tttaactttc agccacgggc caccgtcgat
      961 cagttcaccg ccaaactctt cacgcgccag ctggtagccc cagtctttaa acgctccttc
     1021 ggtgaacttc atgatgttgc ctttgtgcac cagagtcaca gagtcacgat cgttagcaat
     1081 tgcgtattcg atcgctgcac gaaccagacg tttggtgcct tcttccgaac acggcttaat
     1141 accgatacca caatgttccg ggaagcgaat tttcttcacc cccatctctt cacgcaggaa
     1201 tttaatcact ttctcggcgt cggcagagtc tgctttccat tcgatacccg cataaatgtc
     1261 ttccgagttt tcacggaaga taaccatatc ggtcagttca gggtgtttaa ccgggcttgg
     1321 agtgccctga tagtaacgta ccggacgcag gcagatgtag agatccagtt cctggcgcag
     1381 ggcaacgttc agagagcgaa taccgccacc aacaggagtg gtcagcggac ctttaatggc
     1441 aacgcgatat tcacgaatca gatcaagggt ttcagcaggc agccagacat cctgaccata
     1501 aacctgtgtg gatttttcac cggtgtaaat ttccatccag gagattttac gctcgccttt
     1561 ataggctttc tcgactgcag cgtcgaccac tttcagcatg gctggggtta catctacacc
     1621 gattccatca ccttcaatgt aagggataat cggattttca ggaacgttga gtttgccgtt
     1681 ttgcagggtg atcttcttgc cttgtgccgg aacaactact ttactttcca ttcacctctc
     1741 cttcgagcgc tactggtttg ctcgtctgcc accacgttgc atatgcgttt gcgtcctgcg
     1801 atacggatgc tttagagcaa ttttttgtta atgatttgta attggcttgt caatactacc
     1861 ctattgtttg ccgtcatgaa agatgcgcgt tattaggcta taatgcggca attcataatc
     1921 tctgaaaata ccatgcaaaa aacttctttt agaaatcacc aggttaagcg attcagctcg
     1981 caacgttcta ccaggcgtaa acctgaaaac cagcccacgc gtgtgatcct gttcaataaa
     2041 ccctacgatg ttcttccgca gttcaccgat gaagccggac gcaaaacatt aaaagaattc
     2101 atcccggttc agggcgttta tgcagcaggt cgccttgacc gcgatagcga agggttgctg
     2161 gtgctgacca ataacggcgc gttgcaggcg cgtttaaccc agccgggtaa acgcaccgga
     2221 aaaatctatt atgtgcaggt ggaaggtatt cccacacaag acgcacttga agccttgcgc
     2281 aatggcgtaa ccttaaatga tggccctacc ctgcccgcag gcgcggagct ggttgacgaa
     2341 cccgcgtggt tatggccgcg gaatccacca attcgtgaac gcaaaagtat tcccaccagc
     2401 tggctgaaga tcaccttata tgaaggacgt aatcgccagg tgcgccgcat gaccgcccat
     2461 gttggtttcc ccacgctgcg actgattcgc tatgcgatgg gagattactc tttggataat
     2521 cttgccaatg gcgaatggcg agaagtgaca gattaaggat gcaaaatgtt taaaccgcac
     2581 gttaccgttg cttgcgtggt gcacgcagaa ggcaaatttt tagtcgttga agagacgatt
     2641 aatggtaaag cgttatggaa ccaacctgcc gggcatctgg aagccgatga aaccttagtg
     2701 gaagccgccg cccgtgaact gtgggaagaa accggcatca gcgcgcagcc gcaacacttt
     2761 attcgtatgc atcagtggat tgcgccagat aaaacgccgt ttttgcgttt cctctttgcc
     2821 attgagcttg agcaaatatg cccgacccag cctcatgaca gcgatatcga ctgctgccgt
     2881 tgggtcagcg ccgaagaaat tttacaggcg tcaaatcttc gttcgccgct ggtggcggaa
     2941 agtattcgtt gttatcaaag cgggcaacgt tatccgctgg agatgattgg cgattttaac
     3001 tggcctttta caaagggtgt catctaaaag gctgcgtgat agaatacgcc gccttgaagt
     3061 tcaatgtcgt gagtgatcca atgtctgaaa ccgcaaaaaa agtaatcgtc ggcatgtccg
     3121 gcggtgtcga ttcctccgtt tctgcctggc tgttgcaaca acagggatat caggtcgaag
     3181 gcctgtttat gaagaactgg gaagaagacg acggtgagga atattgcaca gcggcagcgg
     3241 atctggctga tgcccaggct gtctgcgaca agctcggcat tgaactgcac accgttaact
     3301 ttgctgccga gtactgggac aacgtcttcg aactgttcct tgccgaatat aaagccggtc
     3361 gcacgccgaa tccggatatt ctgtgcaaca aagagatcaa atttaaagcc ttcctcgaat
     3421 ttgccgccga agatttaggt gccgattata tcgctaccgg tcattacgta cgtcgggccg
     3481 atgtcgatgg caagagccgc ctgctgcgtg gtctggacag caataaagac cagagctact
     3541 tcctttatac gctcagccat gagcagattg cgcaaagcct gttcccggtc ggcgaactgg
     3601 aaaaaccgca ggtgcgtaag attgctgaag atcttggtct ggtcaccgcg aagaaaaaag
     3661 actctaccgg catttgcttc attggcgaac gtaaattccg cgagttccta ggccgttatc
     3721 tcccggcgca accgggcaaa atcattaccg tcgatggcga tgaaattggt gagcaccagg
     3781 ggctgatgta tcacactctc ggtcagcgta aaggtctggg tatcggtggc accaaagaag
     3841 gtaccgaaga accgtggtat gtggtggaca aagacgtcga aaacaacatt ctggttgtcg
     3901 ctcagggcca tgaacacccg cggctgatgt ctgtcgggtt gattgcccag cagttgcact
     3961 gggtcgatcg cgaaccattc accggcacta tgcgttgcac ggtaaaaacc cgctatcgcc
     4021 agaccgatat cccttgcacc gtcaaggcgc tggacgatga tcgcattgaa gtgattttcg
     4081 atgaaccggt tgccgccgtg acgccgggcc agtctgccgt cttctataac ggtgaagtgt
     4141 gcctcggtgg cggtattatt gagcagcgtc tgccgctgcc ggtctgatta ttatctttac
     4201 ttaaaacaag gaagcagtga acgtggcaaa gaattactat gacatcaccc tcgccctggc
     4261 cggtatttgt cagtcggcac gcctggtgca acaactcgct caccaggggc attgtgatgc
     4321 cgatgcgcta cacgtctcac tcaacagtat tattgatatg aaccccagct cgacgctggc
     4381 ggtttttggc ggtagcgaag ccaacctgcg cgtcgggctg gaaaccctgc tcgacgtgct
     4441 caatgccagc agtcgccagg gcttaaacgc cgaattaacc cgctacacac tcagcttgat
     4501 ggtgcttgag cgcaaactct cctcagcgaa aggcgcgctc gacactctgg gcaaccggat
     4561 caacggcctg caacgccagc tcgaacactt cgatttacag tccgaaacgc tgatgagcgc
     4621 gatggctgct atctatgttg atgtgattag cccgcttggc ccgcgcattc aggtcaccgg
     4681 ttcccctgct gtactgcaaa gcccacaagt gcaggcgaaa gttcgcgcaa ccctgctggc
     4741 aggcattcgc gccgccgtgc tctggcacca ggtcggcggc ggacgtctgc aactgatgtt
     4801 ttctcgtaat cgcctgacca ctcaggcaaa acaaattctt gctcatttaa ccccggagtt
     4861 gtgatctatg gaattatcct cactgaccgc cgtttcccct gtcgatggac gctacggcga
     4921 taaagtcagc gcgctgcgcg ggattttcag cgaatatggt ttgctgaaat tccgtgtaca
     4981 agttgaagta cgttggctgc aaaaactggc cgcgcacgca gcgatcaagg aagttcctgc
     5041 ttttgctgcc gacgcaatcg gttaccttga tgcaatcgtc gccagtttca gcgaagaaga
     5101 tgcggcgcgc atcaaaacta tcgagcgtac cactaaccac gacgttaaag cggttgagta
     5161 tttcctgaaa gaaaaagtgg cggagatccc ggaactgcac gcggtttctg aattcatcca
     5221 ctttgcctgt acttcggaag atatcaataa cctctcccac gcattaatgc tgaaaaccgc
     5281 gcgtgatgaa gtgatcctgc catactggcg tcaactgatt gatggcatta aagatctcgc
     5341 cgttcagtat cgcgatatcc cgctgctgtc tcgtacccac ggtcagccag ccacgccgtc
     5401 aaccatcggt aaagagatgg caaacgtcgc ctaccgtatg gagcgccagt accgccagct
     5461 taaccaggtg gagatcctcg gcaaaatcaa cggcgcggtc ggtaactata acgcccacat
     5521 cgccgcttac ccggaagttg actggcatca gttcagcgaa gagttcgtca cctcgctggg
     5581 tattcagtgg aacccgtaca ccacccagat cgaaccgcac gactacattg ccgaactgtt
     5641 tgattgcgtt gcgcgcttca acactattct gatcgacttt gaccgtgacg tctggggtta
     5701 tatcgccctt aaccacttca aacagaaaac cattgctggt gagattggtt cttccaccat
     5761 gccgcataaa gttaacccga tcgacttcga aaactccgaa gggaatctgg gcctttccaa
     5821 cgcggtattg cagcatctgg caagcaaact gccggtttcc cgctggcagc gtgacctgac
     5881 cgactctacc gtgctgcgta acctcggcgt gggtatcggt tatgccttga ttgcatatca
     5941 atccaccctg aaaggcgtga gcaaactgga agtgaaccgt gaccatctgc tggatgaact
     6001 ggatcacaac tgggaagtgc tggctgaacc aatccagaca gttatgcgtc gctatggcat
     6061 cgaaaaaccg tacgagaagc tgaaagagct gactcgcggt aagcgcgttg acgccgaagg
     6121 catgaagcag tttatcgatg gtctggcgtt gccagaagaa gagaaagccc gcctgaaagc
     6181 gatgacgccg gctaactata ttggtcgagc tatcacgatg gttgatgagc tgaaataaac
     6241 ctcgtatcag tgccggatgg cgatgctgtc cggcctgctt attaagatta tccgcttttt
     6301 attttttcac tttacctccc ctccccgctg gtttatttaa tgtttacccc cataaccaca
     6361 taatcgcgtt acactatttt aataattaag acagggagaa ataaaaatgc gcgtactggt
     6421 tgttgaagac aatgcgttgt tacgtcacca ccttaaagtt cagattcagg atgctggcca
     6481 tcaggtcgat gacgcagaag atgccaaaga agccgattat tatctcaatg aacatatacc
     6541 ggatattgcg attgtcgatc tcggattgcc agacgaggac ggtctgtcac tgattcgccg
     6601 ctggcgtagc aacgatgttt cactgccgat tctggtatta accgcccgtg aaagctggca
     6661 ggacaaagtc gaagtattaa gtgccggtgc tgatgattat gtgactaaac cgtttcatat
     6721 tgaagaggtg atggcgcgaa tgcaggcatt aatgcggcgt aatagcggtc tggcttcaca
     6781 ggtcatttcg ctccccccgt ttcaggttga tctctctcgc cgtgaattat ctattaatga
     6841 cgaagtgatc aaactgaccg cgttcgaata caccattatg gaaacgttga tacgcaataa
     6901 tggcaaagtg gtcagcaaag attcgttaat gctacaactc tatccggatg cggagctgcg
     6961 ggaaagccat accattgatg tactgatggg acgtctgcgc aaaaaaattc aggcacaata
     7021 tccgcaagaa gtgattacca ccgttcgcgg ccagggctat ctgttcgaat tgcgctgatg
     7081 aaaaaattac tgcgtctttt tttcccgctc tcgctgcggg tacgttttct gttggcaacg
     7141 gcagcggtag tactggtgct ttcgcttgcc tacggaatgg tcgcgctgat cggttatagc
     7201 gtcagtttcg ataaaactac gtttcggctg ttacgtggcg aaagcaatct gttctatacc
     7261 cttgcgaagt gggaaaacaa taagttgcat gtcgagttac cagaaaatat cgacaagcaa
     7321 agccccacca tgacgctaat ttatgatgag aacgggcagc ttttatgggc gcaacgtgac
     7381 gtgccctggc tgatgaagat gatccagcct gactggctga aatcgaatgg ttttcatgaa
     7441 attgaagcgg atgttaacga taccagcctc ttgctgagtg gagatcattc gatacagcaa
     7501 cagttgcagg aagtgcggga agatgacgac gacgcggaga tgacccactc ggtggcggta
     7561 aacgtctacc cggcaacatc gcgaatgcca aagttaacca ttgtggtggt ggataccatt
     7621 ccggtggagc taaaaagttc ctatatggtc tggagctggt ttatctatgt gctctcagcc
     7681 aatctgctgt tagtgatccc gctgctgtgg gtcgccgcct ggtggagttt acgccccatc
     7741 gaagccctgg caaaagaagt ccgcgaactg gaagaacata accgcgaatt gctcaatcca
     7801 gccacaacgc gagaactgac cagtctggta cgaaacctga accgattgtt aaaaagtgaa
     7861 cgcgaacgtt acgacaaata ccgtacgacg ctcaccgacc tgacccatag tctgaaaacg
     7921 ccactggcgg tgctgcaaag tacgctgcgt tctctgcgta gtgaaaagat gagcgtcagt
     7981 gatgctgagc cggtaatgct ggagcaaatc agccgcattt cacagcaaat tggctactac
     8041 ctgcatcgtg ccagtatgcg cggcgggaca ttgctcagcc gcgagctgca tccggtcgcc
     8101 ccactgctgg acaatctcac ctcagcgctg aacaaagtgt atcaacgcaa aggggtcaat
     8161 atctctctcg atatttcgcc agagatcagc tttgtcggtg agcagaacga ttttgtcgag
     8221 gtgatgggca acgtgctgga taatgcctgt aaatattgcc tcgagtttgt cgaaatttct
     8281 gcaaggcaaa ccgacgagca tctctatatt gtggtcgagg atgatggccc cggtattcca
     8341 ttaagcaagc gagaggtcat tttcgaccgt ggtcaacggg ttgatacttt acgccctggg
     8401 caaggtgtgg ggctggcggt agcccgcgaa atcaccgagc aatatgaggg taaaatcgtc
     8461 gccggagata gcatgcttgg cggtgcgcgg atggaggtga tttttggtcg ccagcattct
     8521 acgccgaaag atgaataaat atgtccatac ttcacgcatt acgttaagca tccgttataa
     8581 tcggttgcag ataccagcct gtggatgctt aacatggaat accaactcac tcttaactgg
     8641 cccgattttc ttgaacgtca ctggcagaaa cgcccggtgg tgttaaaacg cggctttaat
     8701 aattttattg acccgatctc tccagacgag ttggcgggtc tggcgatgga aagcgaagtc
     8761 gacagtcgac tggtcagtca ccatgatggc aaatggcagg tcagccacgg tccgttcgaa
     8821 agctacgatc atctcggtga aactaactgg tcattgttag tgcaagcagt aaatcactgg
     8881 catgagccga ccgccgcgct gatgcgaccg ttccgtgaac taccggactg gcgtattgat
     8941 gatctgatga tctctttttc tgtacctggc ggcggcgtcg gcccgcatct cgatcagtac
     9001 gacgtgttta tcattcaggg taccggacgt cgtcgctggc gagtgggcga aaagctgcaa
     9061 atgaaacagc actgcccaca cccggatctg ttacaggtcg atccgttcga agccatcatc
     9121 gatgaagagc tggagcctgg cgatattctt tatattccgc caggattccc gcatgaaggc
     9181 tacgcgctgg aaaatgcgat gaactattcc gtgggttttc gcgcgccaaa tacgcgggaa
     9241 ttaattagcg gatttgccga ttatgtgctg caacgtgaac tgggcggcaa ctactacagc
     9301 gatccggatg ttccacctcg cgctcatcct gcggacgttc tgccgcaaga aatggataaa
     9361 ctgcgtgaga tgatgctcga attgatcaac cagccggaac actttaagca atggtttggc
     9421 gagtttatat cccagtcacg tcatgaactg gatatcgcgc cgccggaacc gccttatcag
     9481 ccagatgaaa tctacgatgc gctgaaacaa ggtgatgtgc tggtgcgcct gggtggtctg
     9541 cgcgtattgc gcattggcga cgacgtgtat gccaatggtg agaagatcga ttccccgcac
     9601 cgtccggcac tggatgcact cgccagcaac attgcgctga ccgcggagaa ttttggcgat
     9661 gcgctggaag atccgtcatt cctcgcgatg ctcgcggcgc tggtcaatag cggatactgg
     9721 ttcttcgaag ggtaagtttg gatttaagcc ggatgcggca tcacatgccg catccctttt
     9781 cgcttacttc cgttgcgccg ttaactcggc aatacggacg atcacctgca ccgctttttc
     9841 cataccttcc agagtcacaa actcatgctt accatgatag ttgtaaccgc cagtgaacag
     9901 gttcgggcac ggtaatccca taaacgacaa ctgcgcgccg tcggtaccgc cgcggatcgg
     9961 tttcagttcc ggttcaatat cgcagtcacg catcgcctgc tgggcgatat cgagaatatg
    10021 cggatgctca accactttct cgcgcatatt gtagtaactg tcttcaatca ccagttcaat
    10081 gtagcaatca ggatgtaacc ctttgcccac ttttttggcg atctccatca ttttacgttt
    10141 acgcgcttca aactgtttac ggtcgaaatc acggatgatg tagtgcatat cggctcggtc
    10201 aacggtgcct ttcatgctcg ccaggtgata gaaaccttca tagccttctg tcatttccgg
    10261 gctttcatcc gccggaactt ccgcatgaat acgtgccgcc agcgacagcg catttaccat
    10321 cactcctttc gccgtgcccg gatgaacatt gttaccgaca attttgatat tgaccgatgc
    10381 ggcgttgaag ttttcaaact ccagttcgcc tacgccacca ccgtcaacag tgtaagccca
    10441 gcgggcatcg aaggcatcaa catcaaaatg tttcgcccct ttgcccactt cttcatccgg
    10501 ggtaaaggcg acgcgaatat caccatgcgg aatgtttttc tgttgcaata ccgccagcgc
    10561 ggtcatgatt tctgcaatac cggctttgtc atcggcacct aacaaggttt taccatcggt
    10621 ggtaatcaac gtctgaccca gtagctgatg cagcaccggg aacataaccg gtgataaaac
    10681 ttcatcgccg atacccagcg caatatcgcc accgcgatag ttttcaacaa tttgcggatt
    10741 aacatttttg ccgctgcaat ccggtgaggt atccacatga gaaataaagc caatcgccgg
    10801 gatatcgcca gggacgttag ccggtaacgt cgccatcaaa gtgcccttct cacttaaggt
    10861 cacattgata agccccattt cttcgagctg ctctttcagc agatgcaata acttccattg
    10921 gccttccgtg ctgggaacct gtctcacccc tgcttttgat tgggtatcca gagacacgta
    10981 gttcaaaaat cgctcaagta gtttatccat gtagtcaccc tcactttttg tgacaacatt
    11041 attaagaagc aagaaaagac aaatattgcg tcaggtcact tttacccctg caagcgggaa
    11101 tatttatcag cattacctta atgaatataa agctaagcca gtaattcaca acaaagattg
    11161 gcgattcaag cggtttgccg tagaattacc gccctcatta agagtcacca aggtggttaa
    11221 ccacaaaccc cgcatcggta agccatccgt tgcgtttaca tgggacagag taaaaaattg
    11281 aataaacaac cgagttcgct ttcaccgctg gtgcaattgg cgggaattcg caaatgcttt
    11341 gatggtaaag aggtcattcc ccagctggat ctgactatca acaatggcga gttcctcacg
    11401 ctgcttggcc cttctggctg cggtaaaaca accgttcttc gcctgattgc aggtctggaa
    11461 actgttgatt ccggacgcat catgctggat aacgaggaca tcacccacgt tccggcggaa
    11521 aaccgctatg tgaacactgt tttccaaagc tacgcacttt tcccccacat gaccgtgttc
    11581 gaaaatgtgg cctttgggtt gcgcatgcaa aaaacccccg ctgctgaaat tacgccccgc
    11641 gtgatggaag ccctgcggat ggtgcaactg gaaaccttcg ctcaacgtaa accgcatcag
    11701 ctctctggtg gtcaacagca acgcgtcgcc attgctcgcg cggtggttaa caagcctcgt
    11761 ctgttgttgc tggatgagtc gctctcagcg ctggattaca aactgcgtaa gcaaatgcag
    11821 aacgagctga aagcgttaca gcgtaagctt ggtattacct tcgtctttgt cactcacgat
    11881 caggaagaag cgctcaccat gtcagacagg attgtggtga tgcgcgaggg tcgtattgag
    11941 caagatggta caccgcgcga aatttacgaa gagccgaaaa acctgtttgt tgccggcttc
    12001 attggcgaaa tcaatatgtt taacgccact gtcatcgaac gtctagacga gcagcgcgta
    12061 cgcgccaacg ttgaaggccg cgaatgtaat atctacgtta acttcgccgt tgaaccgggg
    12121 caaaaactgc atgttctgct gcgcccggaa gacttacgtg ttgaagagat taacgacgac
    12181 aaccacgctg aagggctgat tggttacgtt cgtgagcgta actacaaagg catgacgctg
    12241 gagtcggttg ttgaactgga aaatggcaag atggtgatgg tcagcgaatt cttcaatgaa
    12301 gacgaccctg actttgacca ctctctcgac caaaaaatgg ccattaattg ggtagaaagc
    12361 tgggaggtcg tactggctga tgaagaacac aagtaagttc cagaatgtag tgattgtcac
    12421 tattgtcggt tggcttgtgt tgtttgtctt tctgcccaac ctgatgatca ttggcaccag
    12481 ctttttgacc cgcgacgacg ccagtttcgt caaaatggtc tttacgctgg ataactacac
    12541 gcgtctgctc gatccgctct attttgaagt gctattgcac tcgctgaata tggcgctgat
    12601 cgccaccctc gcctgcctgg tgctgggcta cccgtttgcc tggtttctgg cgaagctacc
    12661 acacaaggtg cgtccgctgc tgctgtttct gctgattgtt cccttctgga ccaactcact
    12721 gattcgcatc tacgggctga aaattttcct cagcaccaaa ggctatctca acgagttttt
    12781 gctctggctg ggcgttatcg acacaccaat tcgtatcatg ttcacgccca gtgcggtgat
    12841 tatcggtctg gtttacattc tgctaccgtt tatggtgatg ccgctgtact ccagtatcga
    12901 aaaactggat aagccgttgc ttgaggcggc gcgcgatctc ggtgccagca agttacagac
    12961 ttttatccgt atcattattc cactgacgat gccgggaatt attgccggat gtctgctggt
    13021 gatgctgcca gcgatgggcc tgttctatgt gtccgacctg atgggcggtg cgaaaaacct
    13081 gctgatcggt aacgtcatca aggtacagtt cctcaatatt cgtgactggc cgtttggtgc
    13141 agccaccagc attacgctga ctatcgtaat gggcctgatg ttgctggttt actggcgcgc
    13201 ttctcgtctg ctgaataaaa aggtggaact cgaatgatcg gtcgactgct tcgcggcggt
    13261 tttatgaccg ctatctacgc gtacctgtat atcccaatca ttattttgat tgtgaactcc
    13321 tttaacagct cgcgctttgg catcaactgg cagggtttta ccaccaaatg gtatagcctg
    13381 ctgatgaaca acgacagcct gttacaggca gcgcagcatt cactaacaat ggcggtgttt
    13441 tcggcgacat ttgctacgct tatcggttca ctgacggcag ttgcgctgta ccgttatcgc
    13501 tttcgtggta agccgttcgt tagtggaatg ctgtttgtgg tgatgatgtc accagatatc
    13561 gtgatggcga tttcgctgct ggtgctgttt atgctgctgg gtattcagct tggcttctgg
    13621 tcgctgctgt tctcgcatat caccttctgc ctgccatttg tggtggtgac agtgtattcg
    13681 cgcctgaaag gttttgacgt gcgaatgctg gaagcggcga aagatctcgg tgccagcgaa
    13741 tttaccattc tgcgaaaaat cattctgcca ctggcaatgc cagcggtggc ggcgggctgg
    13801 gtgttaagct ttaccctgtc gatggacgat gtagtagttt cttcgttcgt caccggacca
    13861 agttatgaaa ttctgccgtt aaaaatttat tcgatggtca aagtcggcgt atcgccggaa
    13921 gttaacgcgc tggcaaccat attactggtg ctgtcgctgg tgatggttat tgccagccag
    13981 cttattgctc gtgataaaac gaaaggtaac acaggggacg ttaaatgaaa aaatggtcac
    14041 gccacctgct cgcggcgggt gctctggcac tgggcatgag cgccgctcac gccgatgaca
    14101 acaacacgct gtatttctac aactggaccg agtacgtgcc gccaggactg cttgaacagt
    14161 tcaccaaaga aaccggtatt aaggttatct attcgactta cgagtcgaac gaaaccatgt
    14221 atgccaagct gaaaacgtac aaagacggtg cctatgacct ggtggttcct tcaacctatt
    14281 acgtcgataa aatgcgtaaa gaagggatga tccagaagat cgacaagtcg aagttaacga
    14341 acttcagcaa tctcgatcca gacatgctca ataagccgtt tgacccgaat aacgattact
    14401 ccattccgta tatctggggt gcgacggcga ttggtgttaa cggtgatgcg gtggatccga
    14461 aatctgtcac cagctgggcc gatctgtgga agcctgagta caaaggcagc ctgctgttga
    14521 ccgacgatgc ccgtgaagtg ttccagatgg cgctgcgtaa gctgggctac tccggtaaca
    14581 ccaccgatcc gaaagagatt gaagctgcat ataacgagct gaaaaaactg atgccaaacg
    14641 tggcagcctt taactccgat aacccggcta acccgtacat ggaaggcgaa gttaacctcg
    14701 gcatgatctg gaacggttct gctttcgttg cacgccaggc gggtacgcca attgacgtgg
    14761 tatggccgaa agaaggcggc attttctgga tggacagcct ggcgatcccg gcaaatgcca
    14821 aaaacaaaga aggcgcgctg aaattgatca acttcctgct gcgcccggat gtggcaaaac
    14881 aggttgccga aactatcggt tatccaacgc caaaccttgc ggcgcgtaag ctgttaagcc
    14941 cggaagtggc gaacgataaa acgctctacc cggatgctga aaccattaaa aatggcgaat
    15001 ggcagaatga cgttggcgca gccagcagca tttatgaaga gtattatcag aagctgaaag
    15061 caggacgtta attcagcaag cgggcggtgg tattaccgcc cgttttttag ttagcaagga
    15121 tgtggctaat gagtcaggac agtaaagttt tctttcgtat attcctcggt atcggtctgg
    15181 tactgatcct catttctgtt gttgtctttt ataaccagtt cacctatagc aaagacgcga
    15241 ttcataccga aggcgtcatt gttgataccg tctggcactc cagtcattct catcgcacgg
    15301 gtaaagatgg ttcgtggtat cccgtcgttg cctttcgacc gacgccagat tacacgctaa
    15361 ttttcaattc gagtatcggc agcgattttt atgaagacag cgaaggcgat aaagttaatg
    15421 tttattattc tcccgggcat cctgaaaagg ctgagataaa taacccgtgg gttaattttt
    15481 ttaaatgggg gtttatcggc attatgggcg ttatttttat cgccgtcggt ttattaataa
    15541 gtatgccctc ctctaaaaaa tcacgcagga aacggaagtc ccgcccatga aaaaattcat
    15601 tatactgctc agtttactga tactcctgcc tctgacagca gccagcaagc cattaattcc
    15661 aataatgaag acacttttta cggacgttac cggtattgta ccagatgctg aagagatcgc
    15721 gcataaagcc gagctttttc gccaacaaac gggaattgca ctgtttattg tgattttacc
    15781 cgacattaat aatgaagcca gtctcagaca aaatggtaaa gcgatgctgg cgcatgcgtc
    15841 atcttcattg agtaatgtaa aaggaagtgt tctgttacta tttactaccc gcgaaccacg
    15901 gttaattatg atcaccaacg gccaggttga aagtggtctg gacgataaac atctcggcct
    15961 tctgatagaa aatcacacgc tggcttattt aaatgcagat ctctggtatc agggaatcaa
    16021 taatgcattg gctgttctac aagcacagat attaaaacaa tcgacgccgc cactaacgta
    16081 ttatccgcat ccaggacaac agcatgaaaa tgctcccccc ggcagcacca acacattagg
    16141 ttttatcgcc tgggctgcga cgtttatctt attttccagg atcttttact acacgactcg
    16201 ttttatttat gcgttaaaat ttgcagtggc gatgactatt gccaatatgg gttatcaggc
    16261 tttatgtttg tatatcgata atagctttgc gattaccaga atatcgccac tctgggcagg
    16321 gctaattggt gtctgtacgt ttattgccgc attgttattg acgagtaaac gataaaagag
    16381 gccggaaaag tgcgcgtgta ttatgccgga tggggcgcga acgccttatc cggcccacaa
    16441 aacccgcaaa ttcaattaat tgcgtcccct tgcaggcctg ataagcgtag tgcatcaggc
    16501 aatgcttccc gcttttaatc ccttcagcaa cttttcaaca aactcaggca ccacctggct
    16561 tgccgggccg taatatttct cggcaaattc attaccaacc tgactcggtt caagattcag
    16621 ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg ccggataaac
    16681 atgcccggat gtgccaatgg caatgaaaat atcggccatc gacaacgcca tataaatttc
    16741 atccatgccg agtggcattt cgccaaacca cactacatgc gggcgcaagg gtgccggaaa
    16801 ctggcaacaa tggcatttat cttctggggt aacgtctcct gtccagtcga gaacctgacc
    16861 actttgtgaa caacgcactt tcagcagttc cccatgcata tgaatcacat tggtattacc
    16921 tgcgcgttca tgcaggttgt cgatattctg cgtcaccagc aaaaagcgat cgccgagggc
    16981 gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttctg gctgctgcag
    17041 ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat cgaaaccttc
    17101 cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg cgcgaaaggt
    17161 acgaatacct gattccgcag aaattcctgc ccctgtcagt acgagtactc ttggtttttc
    17221 cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac gctcgcgcag
    17281 gcggcgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca gcatagcaac
    17341 ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc ccgcatcacc
    17401 gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac gaggcagcct
    17461 gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa tgacgaccag
    17521 gtcagggtca acaatggtca ggatatttcc cagacaaacc gctaataaat ccagataacg
    17581 ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa agcgcaataa tttcgggagc
    17641 ctgcaacggt tgatgataat agtgttgata cagccacgca aaaccgcgac cagacagata
    17701 attttcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat ccaaccccat
    17761 catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa tgtagctttt
    17821 accggtaatc ggtttgccgt tgaaaatcag cccgccgcca acgccggtgc cgagaatcaa
    17881 ccccatcacc agtggatatt gagtaaattc atcatcccag gcttctgaaa gggcaaaaca
    17941 gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt cggcacgcag
    18001 cggtttaccg ctggcagcgg ggacattggc ggcatacagc gtaccatctt ctgtttccgg
    18061 cattcccgga ataccgatgc cgacagaacc tttacagcca aaacgttggt cagcttcagc
    18121 taccagctca cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg tcggcacccg
    18181 cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt ttgttccacc
    18241 aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt tactggccgc
    18301 taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa cttgccaaaa
    18361 gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc aattccgatg
    18421 gcaggaaatc aataaaatag atatcgctgg agaggaactg atgaccgatc aacttttcaa
    18481 tccactcaat aatcggggta agttgcagtg aaaccactac gccgataatc acaccacaca
    18541 ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga attaaaccat
    18601 ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc accgccatca
    18661 ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc gccagataca
    18721 taatggcgcg gatcatttgg atatcgcgat acatatagcc gtaagtacca atccagcttt
    18781 taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta ttggcgttga
    18841 aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca agatattgtt
    18901 gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc aactgcaaaa
    18961 taccggcaac gtgcaaacgc acacgttttg gctgcatcag tttatgctcg ggattcgagt
    19021 tggggatcat aatcgacacc caatccccct gcttcacttt cagcgcatcc gccacgcctt
    19081 tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccacgcatcg ccctggacaa
    19141 acgagggtaa tgcgctcaga cgctgttcct gttgcgggtt aacgcccttc acctggattg
    19201 cgcgaagatt cgcgccactt tccaccagcc cggtgaaatt gatatacggc gcggcagcgg
    19261 caatacccgg cactttttgc acgtgatcca gtgcttcctg ccagttagtc cacggctgat
    19321 ccaccgcctc gatttcacca tgcggcacca ccgccagaat acggttgttc agttcgcgtt
    19381 caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgccgagg gcaatgccaa
    19441 tggtagaaat aacggagatc agcgacacca tgccgccgcg ccgccgtccg cgactaaaac
    19501 gcaggccaat taataacgat aaaggcatcg ccattactcc gcccccatca ggctcagttc
    19561 cgccgtcaga cgaccatcac gcatttctaa ctggcggctc atacgtttcg ccagttgcag
    19621 gtcgtgagta accaccagga aggcggtgcc ctgcaagcga ttcaattccc caagcaactg
    19681 gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggttcatccg ccagtaccag
    19741 gcgcgggtta ttcaccagcg cacgggcaat cgccacacgc tggcgttcgc cgccagaaag
    19801 ttcagatggg cggtgattcg cacgatgatc cagccccacc gcttttaaca tctcaagtgc
    19861 acggctgttg atttcagcgg gctttttctt gccaatcagc agcggcatag ccacgttttc
    19921 cagggcggta aaatccggca gcaggtggtg aaactgataa ataaagccca gcttctggtt
    19981 gcgcagctct gctttcgccg ccgaagacag tttgctcatc ggttgaccgt taaagatcac
    20041 atcgccggag gttggcgtat ccagcccgcc caacaagtgc agcaaggtgc ttttaccgga
    20101 accagagcta ccgacgatcg ccatcatttc accttcgccg acgctgaaac tgacattgtg
    20161 cagcacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt cgcattgcaa
    20221 caggatctta ttcataacgt aaagcctcag cgggttgagt ggcggcagcg cgccatgaag
    20281 ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca atgacctgta
    20341 aaggttcgat agcgaccggc agcgccgcgc catcaagcag gacgccgatt atcggcatca
    20401 gattatttaa ctggctggca agcagggcgc caagcgccgc tccgaggatc gcaccgataa
    20461 tcccggcgct ggccccttgc accataaaga ccatcatgat ttgtcgcgga gttaagcctt
    20521 gcgtttgcag gatcgctact tcgccctgct tctccattac catcagccct agtgaggtaa
    20581 taatgttaaa cgccgcaacg gcgacaatca ggctcagcag taaacccatc atattttttt
    20641 ccatgcgtac ggcctggaac aactcgcctt tacgatcacg ccagtcctgc catttgctgc
    20701 cttcaggcag tttttgctga cttaatgagt cgactttcag cggctcatcc agccacaaac
    20761 gccagccggt aatattgcct gccggataac gcatcagacg cgaggcatcc tcaatattca
    20821 ccagcatttc attgccatcg acttcactgt tggcggcgaa agtgccaacc acattgaaca
    20881 ggcgctgact tggaatacgc cccatcggcg tgaactggct ggcagatggt accatcatgc
    20941 ggatttgatc accgcgatta acgcctagct gtgaggcaag ttgttcgccg aggatgacat
    21001 tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggc gttagtggat
    21061 ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc cacgctgcgc gcgctttgca
    21121 gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt ttgactgccg
    21181 tttctgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt gcctgtggca
    21241 tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact gacaatactg
    21301 tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag acgaaacgac
    21361 cgaagcgatc cgctgcacgc ccacgcatgt aacgcaggcc aataaataga gcgacaggtt
    21421 ggtacatgaa atccgtctgg ttgctgtagc aaagacacga gtatataagc gaaagcaaga
    21481 tgggtaaacg tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc cccgtgaatc
    21541 aatcggctaa attgccatgt tttatcatca atcttgccgc ctgatcttaa tctgaaaaat
    21601 catccaatac agacagatac ccattacaca tagggtcata acccgcagga tcaaccgcat
    21661 gtaaattaaa aaagagcgtg ttgtcctgat gaaataaaaa gagctatgga ttaaccagat
    21721 caaagggtta tgtatttgtc tggtggtgat ttatcactcg gtcattacct tttatccgca
    21781 tctgaccact tttcagcatc cgttatcgga agtcctgagc aaatgctgga tctatttcaa
    21841 tctttacctt gcaccctttc gtatgccggt ttttttcttt atttccggct atttgattcg
    21901 ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacgcatct ggagcatctt
    21961 ttgggtgctg gcgctttggg gcgtggtgca gtggctggcg ttaagtgcgc ttaaccagtg
    22021 gctggcacct gagcgcgatt taagcaacgc ttccaatgcc gcgtatgccg attccaccgg
    22081 tgagttcctg cacgggatga tcacagccag caccagcttg tggtatctgt atgcgttaat
    22141 tgtctatttc gtggtatgta aaatttttag ccgtctggcg ctgccactat tcgccttgtt
    22201 tgtactgctg agtgtggcgg ttaatttcgt tcccacgccg tggtggggaa tgaacagtgt
    22261 gatccgcaat ttgccttatt acagccttgg cgcatggttt ggcgcaacat taatgacctg
    22321 tgttaaagag gtgccgttgc gccgccatct gctgatggct tctttgctta ccgttctggc
    22381 ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg ctggtatcga ttgtggtgat
    22441 catgaagctg ttttatcaat acgagcaaca attcggtatg cgctccacca gcctgctgaa
    22501 tgtgattggc tccaacacca ttgctatcta caccacccat cgcattctgg ttgaaatatt
    22561 cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg tcgccgcaag tcgaactgac
    22621 actcctgctg ggttacccct ttgttagttt gttcatctgt actgttgcgg gcttgctggt
    22681 aagaaaactt tcacagcgcg cattcagcga tctgttgttc tccccgcctt ctctgcccgc
    22741 ggccatcagt tactcccgct aagccatcgc cccagccggg gcgattcaat ttgctaaacc
    22801 atgtcgatta cggataatga acagcattgc ttatcagaga tatgacccca tatgttgagg
    22861 catatcctaa cgagaatctg acaaccgtta tgcctgaaca atatcgttat acgctgcccg
    22921 tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg cgctgcctgc gcgacgctgg
    22981 tagcggaaat tgccgaacgt cacgccggtc cggtggtact cattgcacca gatatgcaaa
    23041 atgctctgcg tttgcatgat gaaatcagcc agtttaccga tcaaatggtg atgaatctgg
    23101 cggactggga aactcttccc tacgacagtt tttcgcctca tcaggacatt atctcctcgc
    23161 gcctttccac cctttaccag ctaccgacga tgcagcgtgg cgtactgatt gttccggtga
    23221 atacgcttat gcagcgcgtt tgcccgcaca gttttcttca cggtcatgcg ctggtgatga
    23281 aaaaaggtca gcgtctgtca cgagatgcat taagaaccca actggacagc gccggttatc
    23341 gccatgttga ccaggtaatg gagcacggcg aatacgccac gcgcggcgcg ttgctggatc
    23401 tcttcccgat ggggagtgag ctgccttatc gtcttgattt ctttgatgat gaaatcgaca
    23461 gtctgcgggt gtttgacgtc gacagccagc gcacgctgga ggaagtagaa gcgatcaatc
    23521 tgctgcccgc gcacgaattt ccgaccgata aagcggcaat tgaactgttc cgcagccagt
    23581 ggcgcgatac cttcgaagtg aagcgcgatc cagaacatat ttaccagcaa gtgagtaaag
    23641 gcacattacc tgccgggatc gagtactggc agccattgtt cttcagcgaa ccactgccgc
    23701 cgctgttcag ttatttccct gccaatacct tgctcgtgaa tactggcgat ctggaaacca
    23761 gtgccgaacg tttccaggct gacacgctgg cgcgttttga gaatcgcggc gtcgatccga
    23821 tgcgcccgct gttgccacca caatcgctct ggctgcgggt ggacgagctc ttctcagagc
    23881 tgaaaaactg gccccgggtg cagctaaaaa ctgaacattt accgacaaaa gccgcgaatg
    23941 ccaatttagg tttccagaaa ctgccagacc tggccgttca gacgcaacaa aaagcgccgc
    24001 tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc ggtggtgttc tcggtagaaa
    24061 gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg aattaaaatt gctccgcaac
    24121 gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt ggcgctgccg
    24181 aacatggttt tgtcgataag gtgcgtaatc tggcgctgat ctgcgaaagc gatctgctcg
    24241 gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac catcaacccc gatacactga
    24301 tccgtaacct tgcggagctg catattggtc agccggtggt ccatctggag cacggtgtcg
    24361 ggcgctatgc cggaatgacc acgctcgaag cgggcggcat tactggcgag tatttgatgc
    24421 tcacctatgc caacgacgcc aaactgtatg ttccggtgtc gtcactgcat ctgattagcc
    24481 gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa acttggcggc gatgcgtggt
    24541 cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa ttgctggata
    24601 tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt gagcagtatc
    24661 agttgttctg cgacagcttc ccgtttgaaa ccacgccgga tcaggcgcag gccattaatg
    24721 cggtacttag cgacatgtgt cagccgctgg caatggatcg tctggtgtgc ggcgatgttg
    24781 gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct ggcagtagat aaccacaagc
    24841 aggtggcggt gttggttcct accacccttc tcgcgcagca gcattacgac aacttccgcg
    24901 accgtttcgc caactggccg gtacgtatcg aaatgatctc ccgtttccgc agcgccaaag
    24961 agcagacgca aatccttgcg gaagtggcgg aagggaaaat cgatattctg atcggtacgc
    25021 acaaactgct gcaaagtgac gtcaagttta aagatttagg cctgctgatt gtcgatgaag
    25081 aacaccgctt cggggtgcgt cataaagagc gcattaaagc gatgcgcgcg aacgtggata
    25141 ttctgacgct tactgcaacg ccgatcccac gcacgctgaa tatggcaatg agcggaatgc
    25201 gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa acctttgtcc
    25261 gtgagtatga cagcctggtg gtccgggagg cgatcctgcg tgaaattttg cgcggggggc
    25321 aggtttatta tctctacaat gatgtggaaa acatccagaa agctgccgaa cggctggcag
    25381 aactggtgcc agaagcgcgg atcgccatcg gtcacgggca gatgcgcgag cgcgaactag
    25441 aacgggtgat gaatgatttc catcatcaac gtttcaacgt gctggtttgt acaaccatta
    25501 tcgaaaccgg gatcgacatc ccgacagcca acaccattat cattgaacgc gcggatcact
    25561 tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg acgttcacat catcaggcat
    25621 atgcatggct gctgacgccg catccaaaag cgatgactac cgatgcacaa aaacgtcttg
    25681 aagcgattgc ctcgctggaa gatctcggtg caggctttgc gctggcaacg cacgatctgg
    25741 agatccgcgg cgcgggtgaa ctgcttggcg aagaacaaag tggctcaatg gaaaccatcg
    25801 gtttctcgct gtatatggag ttgctggaaa acgccgtcga tgcactgaaa gccggacgcg
    25861 agccgtcgct ggaagatctc accagccagc aaacagaagt cgagctgcgg atgccgtcgc
    25921 tattgccaga tgatttcatt cctgacgtga atacgcgttt gtcgttctat aaacgtattg
    25981 ccagcgccaa aacggaaaac gaactggaag agatcaaagt cgagcttatc gatcgcttcg
    26041 gcctgctgcc ggatccggcg cgtaccctgc tggatgttgc ccgtctgcgc cagcaagcgc
    26101 agaaactggg gatcaggaag ctggaaggta atgagaaagg cggggtgatc gaatttgccg
    26161 agaagaatca cgttaatccg gcctggttga ttggtttgct gcaaaaacag ccgcagcatt
    26221 accgccttga tggtccgacg cgcctgaaat ttattcagga tttgagtgag cggaaaacgc
    26281 gtatcgaatg ggtacgccag tttatgcgtg aactggaaga gaacgcgatc gcttaatggt
    26341 aaatcggggg cgtttctgcg cccccatctg caacatttac aaattctttg cacttccctg
    26401 cactatccga cactgtcacc atccataatt caggcttatc tgtttattac aataacctta
    26461 tatttattat ggatttttgg tgatgatcaa aacgcgtttt tctcgctggc taacgttttt
    26521 tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa gccaacacct ggcagctgcc
    26581 gccagcgggc agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa atgacggtgg
    26641 ttctctggaa gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt tacaggctaa
    26701 ccctggcgtt gatccttacg taccgcgcgc gggtagcgtg ttaacgatcc cgttgcaaac
    26761 cctacttcca gatgcgccgc gcgaaggcat tgtgatcaat attgcggagc tgcgtctcta
    26821 ttactacccg ccgggtaaaa attcggtaac cgtgtatcca ataggtattg gtcagttagg
    26881 tggtgacaca ctgacaccga caatggtgac caccgtttca gacaaacgtg caaacccaac
    26941 ctggacgcca acggcaaaca tccgcgcccg ttataaagca cagggaattg agttgcctgc
    27001 ggtagtgccg gctggaccgg ataacccaat gggccatcat gcgattcgtc tggcggccta
    27061 tggcggcgtt tatttgcttc acggtacgaa cgccgatttc ggcattggca tgcgggtaag
    27121 ttctggctgt attcgtctgc gggatgacga tatcaaaaca ctctttagcc aggtcacccc
    27181 aggcaccaaa gtgaatatca tcaacactcc gataaaagtc tctgccgaac caaacggtgc
    27241 gcgtctggtt gaagtacatc agccgctgtc agagaagatt gatgacgatc cgcagctgct
    27301 gccaattacg ctgaatagcg caatgcaatc atttaaagat gcagcacaaa ctgacgctga
    27361 agtgatgcaa catgtgatgg atgtccgttc cgggatgccg gtggatgtcc gccgtcatca
    27421 agtgagccca caaactctgt aaatcctgga taaaaaaaat ccccgcgaca cggcatgtcg
    27481 cggggaaaaa atcagcagta gcaggcatta atgagggtta atgcttattt ataaattact
    27541 gctgttccat ggagggtatt cggaccggtt acagaagtaa tacggaaaga ttttgcgccc
    27601 atctcatccg ctttttgcgc cagctgctct tccagcgatc ccagatttgt ccccgcgtta
    27661 gcactgattg taccgacttt ttgttggcct tctggcgttg actgaacttc gacagccgca
    27721 aagctggcaa atgacatgga gcttaaaatc gccgcagcga tgagggtttt tacgtttttc
    27781 ataatagtgg ccttatgcag atgaatgacg tcgcaagcaa ttacttaacg atcgat
//
LOCUS       DAJOSY010000059        26954 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJOSY010000059 DAJOSY010000000
VERSION     DAJOSY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.059"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            3..767
                     /gene="ycaL"
                     /locus_tag="OHN27_002581"
     CDS             3..767
                     /gene="ycaL"
                     /locus_tag="OHN27_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCQ2286994.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            940..1623
                     /gene="cmk"
                     /locus_tag="OHN27_002582"
     CDS             940..1623
                     /gene="cmk"
                     /locus_tag="OHN27_002582"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCQ2286995.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1734..3407
                     /gene="rpsA"
                     /locus_tag="OHN27_002583"
     CDS             1734..3407
                     /gene="rpsA"
                     /locus_tag="OHN27_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCQ2286996.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3567..3851
                     /gene="ihfB"
                     /locus_tag="OHN27_002584"
     CDS             3567..3851
                     /gene="ihfB"
                     /locus_tag="OHN27_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCQ2286997.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4059..6323
                     /locus_tag="OHN27_002585"
     CDS             4059..6323
                     /locus_tag="OHN27_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCQ2286998.1"
                     /translation="MKITTVGVCIISGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPASGLVMYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQTSVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTMVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGSLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHIWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQLQGYQWIDTPHQGQTTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6360..8108
                     /gene="msbA"
                     /locus_tag="OHN27_002586"
     CDS             6360..8108
                     /gene="msbA"
                     /locus_tag="OHN27_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCQ2286999.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8105..9091
                     /gene="lpxK"
                     /locus_tag="OHN27_002587"
     CDS             8105..9091
                     /gene="lpxK"
                     /locus_tag="OHN27_002587"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCQ2287000.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            9128..10360
                     /locus_tag="OHN27_002588"
     CDS             9128..10360
                     /locus_tag="OHN27_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCQ2287001.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHINDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHDDLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGCC
                     PQGLFTDCRTGWEIDPVA"
     gene            10412..10594
                     /gene="ycaR"
                     /locus_tag="OHN27_002589"
     CDS             10412..10594
                     /gene="ycaR"
                     /locus_tag="OHN27_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCQ2287002.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10591..11337
                     /gene="kdsB"
                     /locus_tag="OHN27_002590"
     CDS             10591..11337
                     /gene="kdsB"
                     /locus_tag="OHN27_002590"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCQ2287003.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11491..12384
                     /locus_tag="OHN27_002591"
     CDS             11491..12384
                     /locus_tag="OHN27_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCQ2287004.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12361..13140)
                     /gene="elyC"
                     /locus_tag="OHN27_002592"
     CDS             complement(12361..13140)
                     /gene="elyC"
                     /locus_tag="OHN27_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCQ2287005.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            13276..14061
                     /gene="cmoM"
                     /locus_tag="OHN27_002593"
     CDS             13276..14061
                     /gene="cmoM"
                     /locus_tag="OHN27_002593"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCQ2287006.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14058..15380
                     /gene="mukF"
                     /locus_tag="OHN27_002594"
     CDS             14058..15380
                     /gene="mukF"
                     /locus_tag="OHN27_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCQ2287007.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15361..16065
                     /gene="mukE"
                     /locus_tag="OHN27_002595"
     CDS             15361..16065
                     /gene="mukE"
                     /locus_tag="OHN27_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCQ2287008.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            16065..20525
                     /gene="mukB"
                     /locus_tag="OHN27_002596"
     CDS             16065..20525
                     /gene="mukB"
                     /locus_tag="OHN27_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCQ2287009.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            20786..22633
                     /gene="ldtD"
                     /locus_tag="OHN27_002597"
     CDS             20786..22633
                     /gene="ldtD"
                     /locus_tag="OHN27_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCQ2287010.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22814..23362
                     /locus_tag="OHN27_002598"
     CDS             22814..23362
                     /locus_tag="OHN27_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCQ2287011.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23389..24036
                     /gene="gloC"
                     /locus_tag="OHN27_002599"
     CDS             23389..24036
                     /gene="gloC"
                     /locus_tag="OHN27_002599"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCQ2287012.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVIFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24258..25448)
                     /gene="aspC"
                     /locus_tag="OHN27_002600"
     CDS             complement(24258..25448)
                     /gene="aspC"
                     /locus_tag="OHN27_002600"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCQ2287013.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25633..26721)
                     /gene="ompF"
                     /locus_tag="OHN27_002601"
     CDS             complement(25633..26721)
                     /gene="ompF"
                     /locus_tag="OHN27_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCQ2287014.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADIGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         6549 a         6595 c         7302 g         6508 t
ORIGIN      
        1 acatgaagaa tactaaatta ctgctggcga ttgcgacctc tgcagcatta ctgacagggt
       61 gtcaaaatac ccacggtatt gataccaata tggctatcag ctccggttta aatgcctata
      121 aagcagcaac attaagcgat gccgatgcaa aagcgattgc caatcagggc tgtgccgaaa
      181 tggacagcgg caatcaagtc gcaagtaaat ccagcaagta cggtaaacgt ctggcaaaaa
      241 tcgccaaagc attgggtaac aatattaatg gcacgccggt caactataag gtttatatga
      301 ccagcgacgt caacgcatgg gcgatggcga acggctgtgt tcgtgtctac agtggcctga
      361 tggacatgat gaatgacaac gaaattgaag gcgttctggg ccatgaactg ggccacgtcg
      421 cgttgggtca ctcgctggct gaaatgaaag cttcttatgc gatcgttgcc gcacgcgatg
      481 ccatttcagc taccagcggt gtggcttccc agctttcccg ctcacaatta ggtgatatcg
      541 cagaaggcgc tatcaatgct aaatactccc gtgataaaga gtccgaagca gatgatttct
      601 cctttgatct gttgaagaaa cgtggcatca gcacccaggg gctggttggc agctttgaaa
      661 aactggctag cctggatggc ggtcgcaccc agtccatgtt tgactctcac ccaccatcaa
      721 cagagcgtgc gcaacacatc cgtgatcgta tcgcctctgg taagtaaatc attgtcatct
      781 ttcgggctgg tcttctgcca gcccgctata attgcgcaat aaatccccat ctgaatacag
      841 acaaaactgg tttttgcaca caacgttaac gatttgtggc gtcggcgcgt ataatgcgcg
      901 cggttatgtt aacggtacgc ctgttttaag gagataaaga tgacggcaat tgccccggtt
      961 attaccattg atggcccaag cggtgcaggg aaaggcacct tgtgtaaggc tatggcggaa
     1021 gcgttgcaat ggcatctgct ggactcgggt gcaatttatc gcgtactggc attggcggca
     1081 ttacatcacc atgttgatgt tgcgtcggaa gatgcgctgg taccgctggc atcccatctg
     1141 gatgtacgtt ttgtgtcgac caatggcaat ctggaagtga tcctcgaagg ggaagatgtc
     1201 agcggcgaaa ttcgtactca ggaagtggcg aatgcagctt cacaagtcgc ggcattccca
     1261 cgcgttcgtg aagcattatt gcgtcgccaa cgcgcgtttc gcgaattacc aggtctgatt
     1321 gccgatggcc gcgacatggg aacggtggta ttccctgatg caccagtgaa aattttcctt
     1381 gacgcctcct cggaagaacg tgcgcatcgc cgcatgctac agttgcagga gaagggcttt
     1441 agtgttaact ttgagcgcct tttggccgag atcaaagaac gcgacgaccg cgatcgtaac
     1501 cgagcggtag cgccactggt tccggcagcc gatgctttag tgttggattc caccacctta
     1561 agcattgagc aagtgattga aaaagcgcta caatacgcgc gccagaaatt ggctctcgca
     1621 taagcgaccg aatttgcagt acccccgttg caatggaatg acagcgggta tgttaaacaa
     1681 ccccatccgg catggagcca ggtggacgtt aaatataaac ctgaagatta aacatgactg
     1741 aatcttttgc tcaactcttt gaagagtcct taaaagaaat cgaaacccgc ccgggttcta
     1801 tcgttcgtgg cgttgttgtt gctatcgaca aagacgtagt actggttgac gctggtctga
     1861 aatctgagtc cgccatcccg gctgagcagt tcaaaaacgc ccagggcgag ctggaaatcc
     1921 aggtaggtga cgaagttgac gttgctctgg acgcagtaga agacggcttc ggtgaaactc
     1981 tgctgtcccg tgagaaagct aaacgtcacg aagcctggat cacgctggaa aaagcttacg
     2041 aagatgctga aactgttacc ggtgttatca acggcaaagt taagggcggc ttcactgttg
     2101 agctgaacgg tattcgtgcg ttcctgccag gttctctggt agacgttcgt ccggtgcgtg
     2161 acactctgca cctggaaggc aaagagcttg aatttaaagt aatcaagctg gatcagaagc
     2221 gcaacaacgt tgttgtttct cgtcgtgccg ttatcgaatc cgaaaacagc gcagagcgcg
     2281 atcagctgct ggaaaacctg caggaaggca tggaagttaa aggtatcgtt aagaacctca
     2341 ctgactacgg tgcattcgtt gatctgggcg gcgttgacgg cctgctgcac atcactgaca
     2401 tggcctggaa acgcgttaag catccgagcg aaatcgtcaa cgtgggcgac gaaatcactg
     2461 ttaaagtgct gaagttcgac cgcgaacgta cccgtgtatc cctgggcctg aaacagctgg
     2521 gcgaagatcc gtgggtagct atcgctaaac gttatccgga aggtaccaaa ctgactggtc
     2581 gcgtgaccaa cctgaccgac tacggctgct tcgttgaaat cgaagaaggc gttgaaggcc
     2641 tggtacacgt ttccgaaatg gattggacca acaaaaacat ccacccgtcc aaagttgtta
     2701 acgttggcga tgtagtggaa gttatggttc tggatatcga cgaagaacgt cgtcgtatct
     2761 ccctgggtct gaaacagtgc aaagctaacc cgtggcagca gttcgcggaa acccacaaca
     2821 agggcgaccg tgttgaaggt aaaatcaagt ctatcactga cttcggtatc ttcatcggcc
     2881 tggacggcgg catcgacggc ctggttcacc tgtctgacat ctcctggaac gttgcaggcg
     2941 aagaagcagt tcgtgaatac aaaaaaggcg acgaaatcgc tgcagttgtt ctgcaggttg
     3001 acgcagaacg tgaacgtatc tccctgggcg ttaaacagct cgcagaagat ccgttcaaca
     3061 actgggttgc tctgaacaag aaaggcgcta tcgtaaccgg taaagtaact gcagttgacg
     3121 ctaaaggcgc aaccgtagaa ctggctgacg gcgttgaagg ttacctgcgt gcttctgaag
     3181 catcccgtga ccgcgttgaa gacgctaccc tggttctgag cgttggcgac gaagttgaag
     3241 ctaaattcac cggcgttgat cgtaaaaacc gcgcaatcag cctgtctgtt cgtgcgaaag
     3301 acgaagctga cgagaaagat gcaatcgcaa ctgttaacaa acaggaagat gcaaacttct
     3361 ccaacaacgc aatggctgaa gctttcaaag cagctaaagg cgagtaattc tctgactctt
     3421 cgggattttt attccgaagt ttgttgagtt tacttgacag attgcaggtt tcgtcctgta
     3481 atcaagcact aagggcggct acggccgccc ttaatcaatg cagcaacagc agccgcttaa
     3541 tttgccttta aggaaccgga ggaatcatga ccaagtcaga attgatagaa agacttgcca
     3601 cccagcaatc gcacattccc gccaagacgg ttgaagatgc agtaaaagag atgctggagc
     3661 atatggcctc gactcttgcg cagggcgagc gtattgaaat ccgcggtttc ggcagtttct
     3721 ctttgcacta ccgcgcacca cgtaccggac gtaatccgaa gactggcgat aaagtagaac
     3781 tggaaggaaa atacgttcct cactttaaac ctggtaaaga actgcgcgat cgcgccaata
     3841 tttacggtta agttttttac tcaaacttga acgagagaaa agcacctgtc gggtgctttt
     3901 ttcatttctc taatctggaa ctggaagctg cctcgcagag ttttgaacag ttttcaccct
     3961 ttcgttaaat tcttctgaat atgcctcggg gaacgcaaaa ttcccaccag acaaccgctc
     4021 aacaaagttg cacactttcc ataaacaggg aggggtgcat gaaaataacg acagtcggtg
     4081 tatgcataat tagcggaatt tttccgttgc tgattttgcc ccaattgcct gggacattaa
     4141 cccttgcgtt tctgactctc ttcgcctgcg tactggcatt tatccctgtt aaaaccgtcc
     4201 gttatatcgc gctgacgttg ctgtttttcg tttggggcat attatcagca aagcaaattt
     4261 tgtgggcagg agaaacctta actggcgcga cgcaggatgc aattgttgag atcactgcaa
     4321 ctgacggcat gaccactcat tacggtcaaa ttactcatct acaaggtcga cgtatattcc
     4381 ctgcgtcagg tctcgtgatg tatggcgaat atcttccgca agcggtttgt gctggacaac
     4441 aatggtcaat gaaactcaaa gttcgtgcag ttcatggcca acttaatgat ggcggctttg
     4501 atagccagcg ttatgccatt gcccagcatc agccgctcac cggccgtttt ctgcagacaa
     4561 gtgtcattga accgaattgt agcctgcgtg cacagtatct ggcgtcacta caaacaacgc
     4621 tgcaacccta tccgtggaat gcggttattc ttggtttagg tatgggggaa cggttatccg
     4681 tccccaaaga aatcaaaaat atcatgcgcg atactggaac ggcgcattta atggcgatat
     4741 cgggattgca catcgctttt gcggcgttgc tggctgccgg actcattcgc agtggacaaa
     4801 tttttctgcc tgggcgctgg atccactggc aaataccatt aattggcgga atctgctgtg
     4861 ctgcttttta tgcctggttg acgggaatgc aacctcctgc attgcgtacc atggtggcgc
     4921 ttgctacgtg gggaatgctt aagttaagtg ggcgacagtg gagtggctgg gatgtatgga
     4981 tatgttgtct ggcggcaatt ttgctgatgg atcctgttgc cattctctcg caaagtttat
     5041 ggctctctgc cgctgcggtc gcggcattga tattttggta tcagtggttt ccctgtcctg
     5101 agtggcaact gccgccggta ttgcgtgcag ttgtttccct catccatctg caactgggaa
     5161 tcacacttct gcttatgccc gtgcaaatcg tcatatttca tggcattagt ctgacctcgt
     5221 ttattgcaaa tctattagca attcccttgg tgacatttat cacggttccg ttgatcctcg
     5281 ccgcgatggt tgtgcattta agcgggccgt taatcctgga gcaagggtta tggtttcttg
     5341 ccgaccggtc tttggcttta cttttctggg ggttaaagag tttgccggaa gggtggatca
     5401 acattgctga atgttggcaa tggctatcat tttccccatg gttcttactg gtggtatggc
     5461 gattaaacgc ctggcgaacg ttgccagcaa tgtgtgtggc tggaagcttg ctgatgtgct
     5521 ggccgctgtg gcaaaaacct cgacctgacg agtggcagct gtacatgctt gatgtcgggc
     5581 aagggctggc aatggtgata gccagaaacg gcaaagcgat tctctatgac acaggactgg
     5641 cctggcctga aggggatagt gggcaacaac tgattatccc ctggctccac tggcataatc
     5701 ttgaaccgga aggcgttatt ctgagccatg aacatctgga tcaccgggga gggctggatt
     5761 caatattgca tatatggccg atgttatgga tcagaagtcc gttaaactgg gaacatcatc
     5821 agccctgtgt gcgtggcgaa gcgtggcaat ggcaaggatt gcgtttcagc gcgcactggc
     5881 ctttacaagg tagcaacgat aaaggaaata accattcctg tgtggttaag gttgatgacg
     5941 ggacgaatag cattcttcta accggtgata ttgaagcccc agctgaacaa aagatgctaa
     6001 gccgttactg gcagcaagtg caggcaacat tgcttcaggt acctcaccat ggcagtaata
     6061 cctcatcatc gttgccatta attcagcgag tgaatggaaa agtggcactc gcatcggcat
     6121 cgcgctataa cgcatggcga ctgccctcta acaaagttaa gcatcgctat caactgcaag
     6181 gatatcaatg gattgatact ccacatcaag gtcaaacaac ggtcaatttt tcagcgcaag
     6241 gctggcggat tagcagcctc agggagcaaa ttttacctcg ttggtatcat cagtggtttg
     6301 gcgtgccagt ggataacggg tagaatatgc ggctatttca acaaatgctg gttttttgaa
     6361 tgcataacga caaagatctc tctacgtggc agacattccg ccgactgtgg ccaaccattg
     6421 cgcctttcaa agcgggtctg atcgtggcgg gcgtagcgtt aatcctcaac gcagccagcg
     6481 ataccttcat gttatcgctc cttaagccac ttcttgatga tggctttggt aaaacagatc
     6541 gctccgtgct ggtgtggatg ccgctggtgg tgatcgggct gatgatttta cgtggtatca
     6601 ccagctatgt ctccagctac tgtatctcct gggtatcagg aaaggtggta atgaccatgc
     6661 gtcgccgcct gtttggtcac atgatgggaa tgccagtttc attctttgac aaacagtcaa
     6721 cgggtacgct gttgtcacgt attacctacg attccgaaca ggttgcttct tcttcttccg
     6781 gcgcactgat tactgttgtg cgtgaaggtg cgtcgatcat cggcctgttc atcatgatgt
     6841 tctattacag ttggcaactg tcgatcattt tgattgtgct ggcaccgatt gtttcgattg
     6901 cgattcgcgt tgtatcgaag cgttttcgca acatcagtaa aaacatgcag aacaccatgg
     6961 ggcaggtgac caccagcgca gaacaaatgc tgaagggcca caaagaagta ttgattttcg
     7021 gtggtcagga agtggaaacg aaacgctttg ataaagtcag caaccgaatg cgtcttcagg
     7081 ggatgaaaat ggtttcagcc tcttccatct ctgatccgat cattcagctg atcgcctctt
     7141 tggcgctggc gtttgttctg tatgcggcga gcttcccaag tgtcatggat agcctgactg
     7201 ccggtacgat taccgttgtt ttctcttcaa tgattgcact gatgcgtccg ctgaaatcgc
     7261 tgaccaacgt taacgcccag ttccagcgcg gtatggcggc ttgtcagacg ctgtttacca
     7321 ttctggacag tgagcaggag aaagatgaag gtaagcgcgt gatcgagcgt gcgactggcg
     7381 acgtggaatt ccgcaatgtc acctttactt atccgggacg tgacgtacct gcattgcgta
     7441 acatcaacct gaaaattccg gcagggaaga cggttgctct ggttggacgc tctggttcgg
     7501 gtaaatcaac catcgccagc ctgatcacgc gtttttacga tattgatgaa ggcgaaatcc
     7561 tgatggatgg tcacgatctg cgcgagtata ccctggcgtc gttacgtaac caggttgctc
     7621 tggtgtcgca gaatgtccat ctgtttaacg atacggttgc taacaacatt gcttacgcac
     7681 ggactgaaca gtacagccgt gagcaaattg aagaagcggc gcgtatggcc tacgccatgg
     7741 acttcatcaa taagatggat aacggtctcg atacagtgat tggtgaaaac ggcgtgctgc
     7801 tctctggcgg tcagcgtcag cgtattgcta tcgctcgagc cttgttgcgt gatagcccga
     7861 ttctgattct ggacgaagct acctcggctc tggataccga atccgaacgt gcgattcagg
     7921 cggcactgga tgagttgcag aaaaaccgta cctctctggt gattgcccac cgcttgtcta
     7981 ccattgaaaa ggcagacgaa atcgtggtcg tcgaggatgg tgtcattgtg gaacgcggta
     8041 cgcataacga tttgcttgag caccgcggcg tttacgcgca acttcacaaa atgcagtttg
     8101 gccaatgatc gaaaaaatct ggtctggtga atcccctttg tggcggctat tgctgccact
     8161 ctcctggttg tatggcctgg tgagtggcgc gatccgtctt tgctataaac taaaactgaa
     8221 gcgcgcctgg cgtgcccccg taccggttgt cgtggttggt aatctcaccg caggcggcaa
     8281 cggaaaaacc ccggtcgttg tctggctggt ggaacagttg caacagcgcg gtattcgcgt
     8341 gggggtcgta tcgcggggat atggtggtaa ggctgaatct tatccgctgt tattgtcggc
     8401 agataccaca acagcacagg cgggtgatga acctgtgttg atttatcaac gcactgatgc
     8461 gcctgttgcg gtttctcccg ttcgttctga tgcggtaaaa gccattctgg cgcaacaccc
     8521 tgatgtgcag atcatcgtaa ccgacgacgg tttacagcat taccgtctgg cgcgtgatgt
     8581 ggaaattgtc gttattgatg gtgtgcgtcg ctttggcaat ggctggtggt tgccggcggg
     8641 gccaatgcgt gagcgagcgg ggcgcttaaa gtcggttgat gcggtaatcg tcaacggcgg
     8701 tgtccctcgc agcggtgaaa tccccatgca tctgctgccg ggtcaggcgg tgaatttacg
     8761 taccggtacg cgttgtgacg ttgctcagct tgaacatgta gtggcgatgg cggggattgg
     8821 gcatccgccg cgcttttttg ccacgctgaa gatgtgtggc gtacaaccgg aaaaatgtgt
     8881 accgctggcc gatcatcagt ctttgaacca tgcggatgtc agtgcgttgg taagcgccgg
     8941 gcaaacgctg gtaatgactg aaaaagatgc ggtgaaatgc cgggcctttg cagaagaaaa
     9001 ttggtggtat ttgcctgtag acgcacagct ttcaggtgat gaaccagcga aactgcttac
     9061 gcaactaacc tcgctggctt ctggcaacta gttacgccgc ggcagcgttc gattgatgga
     9121 gtcatgaatg tcgctgccgc acctctccct tgctgatgcg cgtaatcttc accttgccgc
     9181 acaaggcctg ttaaacaaac cccgccgtcg agcgtcgttg gaggatattc cggcaacgat
     9241 ctcccgcatg tccttgctgc aaatcgatac catcaatatt gttgcccgta gtccatatct
     9301 ggtgcttttc agtcgtctgg gaaattatcc tgcccagtgg ctggatgagt ctctggcgcg
     9361 tggcgaatta atggaatact gggcgcatga agcctgcttt atgccgcgta gcgactttcg
     9421 tcttattcgc caccgcatgc tggcacctga aaaaatgggc tggaaataca aagacgcctg
     9481 gatgcaggaa catgaggcgg aaattgcaca gttaattcag catattaatg ataaggggcc
     9541 ggtacgttca gccgattttg agcatcctcg taaaggtgca agcggctggt gggaatggaa
     9601 gccgcataaa cggcatctgg aaggtttatt tactgccgga aaggtgatgg tgattgaacg
     9661 gcgcaacttc cagcgcgttt atgatttaac ccaccgtgtc atgcctgact gggatgatga
     9721 gcgcgatctc gtttcgcaaa cagaagcaga aatcatcatg ctggataaca gtgcgcgtag
     9781 cctgggaata ttccgcgaac agtggctggc agattactat cggctgaaac gtccggcact
     9841 ggcggcgtgg cgcgaagcga gggctgaaca gcagcaaatc attgctgtgc atgttgaaaa
     9901 attgggcaat ctttggctgc atgatgattt gctgccgcta ctcgagcgag cgctggccgg
     9961 aaagctcact gcaacgcaca gcgcggtact ttcgcctttt gatcctgttg tctgggatcg
    10021 caaacgcgca gagcagcttt ttgattttag ctaccggctg gagtgctata ccccagcgcc
    10081 gaaacgccag tatggctatt ttgttctgcc gttattacat cgtgggcaat tagttgggcg
    10141 aatggatgcc aaaatgcatc gccagacagg catccttgaa gttatctctc tgtggttaca
    10201 ggaaggtatt aaaccaacga caacgctgca aaaagggcta cgtcaggcga ttactgattt
    10261 cgctaactgg cagcaggcaa cgcgggtgac attaggatgc tgcccgcaag gcctctttac
    10321 tgattgccgc accggctggg aaatagaccc cgtcgcataa atgattatga taagatttat
    10381 gcattcataa cccgatccat ctggaggaac tatggatcat cgtctgcttg aaatcattgc
    10441 ctgcccggtt tgcaacggaa aactttggta taaccaggaa aaacaagagc ttatttgcaa
    10501 actggacaac ctcgctttcc cgctgcgtga tggcattcca gtgttgctgg aaacggaagc
    10561 ccgcgtgctg actgctgatg agagtaaatc atgagttttg tggtcattat tcccgcgcgc
    10621 tacgcgtcca cgcgtctgcc cggtaaacca ttggttgata ttaacggcaa acccatgatt
    10681 gttcatgttc ttgaacgcgc gcgtgaatca ggtgccgagc gcatcatcgt ggcaaccgat
    10741 catgaggatg ttgcccgcgc cgttgaagcc gctggcggtg aagtatgtat gacgcgcgcc
    10801 gatcatcagt caggaacaga acgtctggcg gaagttgtcg aaaaatgcgc attcagcgac
    10861 gacacggtga tcgttaatgt gcagggtgat gaaccgatga tccctgcgac aatcattcgt
    10921 caggttgctg ataacctcgc tcagcgtcag gtgggtatgg cgactctggc ggtgccaatc
    10981 cacaatgcgg aagaagcgtt taacccgaat gcggtgaaag tggttctcga cgctgaaggg
    11041 tatgcactgt acttctctcg cgccaccatt ccttgggatc gtgatcgttt tgcagaaggc
    11101 cttgaaaccg ttggcgataa cttcctgcgt catcttggta tttatggcta ccgtgcaggc
    11161 tttatccgtc gttacgtcaa ctggcagcca agtccgttag aacacatcga aatgttagag
    11221 cagcttcgtg ttctgtggta cggcgaaaaa atccatgttg ctgttgctca ggaagttcct
    11281 ggcacaggtg tggatacccc tgaagatctt gagcgcgttc gcgctgaaat gcgctaattt
    11341 cacttcacga cacttcagcc aattttggga ggagtgtcgt accgttacga ttttcctcaa
    11401 tttttctttt caacaattga tctcattcag gtgacatctt ttatattggc gctcattatg
    11461 aaagcagtag cttttatgag ggtaatctga atggaacagc tgcgtgccga attaagccat
    11521 ttactgggcg aaaaactcag tcgtattgag tgcgtcaatg aaaaagcgga tacggcgttg
    11581 tgggctttgt atgacagcca gggaaaccca atgccgttaa tggcaagaag ctttagtacg
    11641 cccggaaaag cccgacaact ggcatggaaa accaccatgc tggcaagaag tgggactgtc
    11701 cgtatgccga ctatttatgg tgtgatgacg catgaagaac accccggccc cgatgtcctg
    11761 ctactggagc ggatgcgtgg tgtttcggtg gaggcaccag cccgaacacc agaacgctgg
    11821 gaacaactca aagaccaaat cgttgaagcc ttactggcct ggcaccgtca ggacagtcgc
    11881 ggttgcgtcg gcgcggtcga caatactcag gaaaatttct ggccctcatg gtaccggcaa
    11941 catgttgaag tgctatggac cacgctcaat cagttcaata acaccggtct gacgatgcag
    12001 gataagcgga tcctgtttcg cactcgcgaa tgtctcccgg cattatttga aggctttaac
    12061 gacaattgtg tgctgattca cggtaacttc tgtttacgca gcatgttgaa agattcgcgc
    12121 agcgatcagt tactggcgat ggtcgggccg ggactaatgc tttgggcacc gcgagaatac
    12181 gaactgttcc gactaatgga taattctctg gcggaagatt tgctctggag ttacctgcaa
    12241 cgcgcgccag tggcggagtc gttcatctgg cggcgttggt tgtatgtgtt atgggatgaa
    12301 gttgcgcaac tggttaatac cggacgattt agtcggcgca acttcgatct ggcatcaaaa
    12361 tcactcttgc cgtggctcgc ctgacgaacc ttttagccac tgccagatac ggccaagcgt
    12421 ttcatagcca acacgatcgc tatgcatcaa ccagaccgga gaagggattg cccgttccca
    12481 cgggttaagc ggcgagtcga tggccaactg attcgccggg gctggtagcg gatttaaccc
    12541 ttcctgctga aaaaagatca tcgcccgcgg cagatgtgat gcggaggtca ccagcaggaa
    12601 aggggcgtca ccaatcgcct gtttcactgc tgcagcttct tcttcggtat cttttggcaa
    12661 atccagggtg ataatttgct cgcgcggcac gcccagcgat tgcgcaactc tggcacctac
    12721 ttccgctgta ctcaccgtat tggtttttgc tacgcctccc gtgaagatca gttttgatcc
    12781 cggattttcg cgccataagc gaataccttc attcaggcgt ggcagactgt tattgattaa
    12841 attagagctc ggtgcccact gcgggttcca ggtataacca cctccgagca ccacgatata
    12901 gtccactttt tgcgaattat tccatgtcgg ataggtgctt tcgatgggac gcagcaggcg
    12961 gtcggctacc ggttgcaaac tcaataacaa gagcgccagc cacccaatgc taataaaaat
    13021 cttcccggtt ttttgaaaac ggctaaacca cagcagcgcc aggccagcgc caataatcaa
    13081 caacatcagc ggaagtggca gcaacatatt gccaatcact tttttcagtg taaaaagcat
    13141 cctttctggt tcctttttta accatatagc aagggatcgc cagcgatatt acaccagacg
    13201 ggttcattct cgccgtggct gtgacaaaat agcgtcttta tcagaaaacg tcttagccaa
    13261 ccggtggaga aagcaatgca ggatcgcaat tttgatgata ttgcggaaaa gttttcccgt
    13321 aacatttacg gcaccaccaa agggcagctt cgacaggcta ttctgtggca ggatctcgat
    13381 cgcgtgctgg cggaaatggg cccgcaaaaa ctgcgtgtgc tggatgctgg cggtggagaa
    13441 gggcagaccg caatcaaaat ggccgagcgt gggcatcagg tcattttatg cgatctttct
    13501 gcgcagatga tcgaccgcgc aaaacaggcg gcagaagcaa aaggtgtgag cgacaacatg
    13561 caatttatac attgcgccgc tcaggatgtt gcttcgcatt tggaaacgcc cgttgatctg
    13621 atattgttcc atgcggtgct cgagtgggtg gctgatcccc gcagcgtatt gcagaccctg
    13681 tggtcagtgt tgcgtccagg cggcgtgttg tcgttaatgt tctacaatgc gcatggtttg
    13741 ttgatgcata acatggtcgc cgggaatttt gattacgtgc aggcgggaat gccgaaaaag
    13801 aaaaaacgga cgctttcgcc agattatcca cgcgacccgg cgcaggttta tctgtggctg
    13861 gaagaagctg gttggcaaat tatgggtaag acaggcgttc gcgtgtttca tgattatctg
    13921 cgcgagaaac accagcagcg cgactgctat gaagcattac ttgaattaga aacgcgttat
    13981 tgccgtcagg aaccgtatat taccctgggg cgttatattc atgtcaccgc gcgcaaaccg
    14041 cagagcaagg ataaagtatg agtgaatttt cccagacagt ccccgaactg gttgcctggg
    14101 ccagaaaaaa tgacttctcc atctcgctgc cggtagaccg actctctttt ctgctggcgg
    14161 ttgccacgct gaacggcgag cgtctggatg gtgagatgag tgaaggcgag ctggtggatg
    14221 cattccgcca tgtgagtgat gcgtttgagc aaaccagcga aaccatcggc gtgcgcgcca
    14281 ataacgcgat caacgacatg gtgcgtcaac gtctgctgaa ccgctttacc agcgagcagg
    14341 cggaagggaa cgcaatttac cgtctgacgc cgctcggcat cggcattact gactactaca
    14401 tccgtcagcg cgagttttct acgctgcgtc tttctatgca gttgtcgatt gtggcgggtg
    14461 agctcaaacg cgcagcagat gccgccgaag agggcggtga tgaatttcac tggcaccgta
    14521 atgtctatgc gccactgaaa tattcggtag cagaaatttt cgacagtatc gacctgacgc
    14581 aacgtctgat ggacgaacag cagcagcagg tgaaggacga tatcgcccag ttgctgaaca
    14641 aagactggcg ggcggcgatt tccagctgtg aattgttgct ttcggaaact tccggaacgc
    14701 tgcgtgaatt gcaggatacg ctggaagcgg caggcgacaa attgcaggct aatctgttgc
    14761 gcattcagga tgcgacgatg acccatgacg atctgcattt cgtcgatcgt ctggtgttcg
    14821 atctgcagag caaactcgat cgtattatca gttggggcca gcaatccatc gacttgtgga
    14881 ttggctacga ccgccacgta cacaaattta ttcgtaccgc gatcgatatg gataaaaacc
    14941 gcgtctttgc tcagcggtta cgtcagtcgg tacaaaccta ttttgatgag ccgtgggcgc
    15001 taacttatgc caatgccgat cgtctgctgg atatgcgtga cgaagagatg gcactgcgcg
    15061 atgaagaagt gactggggaa cttcctgagg atctggaata cgaagagttt aacgagatcc
    15121 gcgaacagct ggcggcgatc atcgaagaac aacttgccgt gtacaaaacc agacaagtgc
    15181 cgctggatct tggtctggtg gtacgcgaat atctgtcaca gtatccgcgt gcacgtcact
    15241 ttgacgttgc gcgtattgtt attgatcagg cggtacgtct tggcgtagcg caagcagatt
    15301 tcaccggact gccagcgaaa tggcagccga ttaatgatta cggagccaag gtacaggcgc
    15361 atgtcatcga caaatattga acaagtgatg ccggttaagc tggcgcaggc gctggcgaat
    15421 ccgttatttc cggcgctgga cagcgcctta cgttcaggac gccatattgg cctcgacgaa
    15481 ctggataatc atgcattcct gatggatttt caggaatatc tggaagagtt ttacgcgcgt
    15541 tataacgttg agcttattcg cgcaccggaa gggttcttct atttacgccc acgttccacc
    15601 actcttatcc cgcgttccgt cttgtcggaa ctggatatga tggtcgggaa aatcctctgt
    15661 tatctctatc tcagcccgga acggctggcg aatgagggga ttttcaccca gcaggaactg
    15721 tacgacgaac tgctcaccct ggccgatgaa gcaaaactgc tgaaactggt gaacaaccgt
    15781 tcaaccggtt cagacgttga ccgtcagaag ttgcaggaga aagtacgttc ttcgctcaac
    15841 cgtctgcgtc gtttaggcat ggtgtggttt atgggccacg acagcagcaa gttccgcatt
    15901 accgaatcgg tgttccgctt cggagccgat gtgcgtgctg gcgacgatcc ccgtgaagca
    15961 cagcgtcgcc tgattcgtga tggcgaagca atgccgattg aaaatcatct gcaactcaac
    16021 gatgaaaccg aagagagcca gccagatagc ggagaggaag aataatgatt gaacgcggta
    16081 aatttcgctc actgacgctg attaactgga acggcttttt tgcccgaact tttgaccttg
    16141 acgagctggt cacgacgctt tccggcggta acggggcggg taaatccacc acgatggcgg
    16201 cgttcgttac ggcgctgatc cccgacctga ccctgctgca tttccgtaac actacggaag
    16261 ccggggccac cagcggttcg cgcgataaag gtctgcacgg taagctgaaa gcgggtgtct
    16321 gttattcgat gctcgacacc attaactcgc gccaccagcg cgtggtggtc ggtgtgcgtc
    16381 tgcaacaggt tgccggacgc gatcgtaaag tggatatcaa accatttgcc attcagggac
    16441 tgccgatgtc ggtgcagccg acacagctgg tgaccgaaac cctgaacgaa cgccaggcgc
    16501 gcgtgctgcc gcttaacgag ctgaaagaca agctcgaggc gatggaaggc gtgcagttta
    16561 aacagttcaa ctccattacc gattaccact cgctgatgtt cgatctgggc atcatcgcgc
    16621 gtcgtctgcg ctccgcatct gaccgtagca aattctatcg tctgatcgaa gcttcgctgt
    16681 atggcgggat ctccagtgcc attacccgtt ctctgcgcga ctacctgttg ccggaaaaca
    16741 gcggcgtgcg taaagcgttc caggacatgg aagcggcgct gcgtgaaaac cgcatgacgc
    16801 tggaagcgat tcgtgtcacc cagtcggacc gtgacctgtt taagcatctg atcagcgaag
    16861 ctaccaacta cgtggcggcg gactacatgc gtcacgccaa cgagcgccgt gtccatctcg
    16921 acaaagccct ggagtttcgt cgcgagttac atacttcgcg tcagcaactg gcggctgagc
    16981 agtacaaaca cgtcgatatg gcgcgtgagc tggcagagca caacggtgcc gaaggcgatc
    17041 tggaagcgga ttatcaggcg gccagcgatc acctgaacct ggtgcaaacc gcactgcgtc
    17101 agcaggagaa gatcgaacgc tacgaagcgg atctcgatga gctgcagatc cgtctggaag
    17161 agcaaaacga agtggtggca gaagccatcg aacgccagga agagaacgag gctcgcgcgg
    17221 aagctgccga actggaagtg gacgagctga aaagccagct tgctgactac cagcaggcgc
    17281 tggacgtcca gcaaacgcgc gcgatccagt ataaccaggc gattgctgca cttaatcgtg
    17341 ccaaagaact gtgccatctg ccggacttaa ccgccgacag cgccgccgaa tggctggaaa
    17401 ccttccaggc gaaagagctg gaagcgactg aaaaaatgct ctctcttgag cagaaaatga
    17461 gcatggcgca aaccgcgcac agccagtttg agcaggctta tcagctggtg gtggcaatca
    17521 acggcccact ggcgcgtaac gaggcgtggg atgtcgctcg cgaactattg cgcgaagggg
    17581 tcgatcagcg tcacctggca gagcaggttc agccgctgcg gatgcgatta agcgaactgg
    17641 aacagcgtct acgcgagcag caagaagctg agcgtctgct ggcagatttc tgcaaacgtc
    17701 agggcaagaa ttttgatatc gacgaactgg aagccctgca tcaggaactg gaagcacgca
    17761 ttgcctctct ttccgatagc gtgtctaacg cccgtgaaga gcgcatggca ctgcgccagg
    17821 agcaggaaca gctgcagtct cgcattcaga gtttgatgca gcgtgcgccg gtttggctgg
    17881 cagcgcaaaa cagtctcaac cagttgagcg aacagtgcgg cgaagagttt acctccagcc
    17941 aggacgtcac cgaatatctg caacagttgc tggagcgtga gcgagaggcg attgttgaac
    18001 gcgatgaagt gggcgcgcgc aaaaacgccg tcgatgaaga gatcgaacgt ttaagccagc
    18061 ctggcggctc tgaagatcag cgtctgaacg cgctggcgga gcgttttggt ggtgtgctgc
    18121 tgtcagaaat ttatgacgac gttagcctgg aagatgcgcc gtacttctca gcgctgtatg
    18181 gcccgtcacg ccacgccatc gtggtgccag atctgtcaca ggtaactgaa cacctggaag
    18241 gcttgaccga ttgcccggaa gatctctatc tgatcgaagg agatccgcag tcattcgatg
    18301 acagcgtgtt cagcgttgat gagctggaaa aagcggtagt ggtgaaaatc gccgatcgtc
    18361 agtggcgtta ttcacgtttc ccggaagtgc cgctgtttgg tcgtgctgcg cgtgaaagcc
    18421 gtattgaaag cctccatgcc gagcgtgaag tgctttccga acgcttcgcc acgctctcct
    18481 ttgatgtaca gaaaactcag cgtctgcatc aggcgttcag ccgctttatc ggcagtcatc
    18541 tggcggttgc gtttgagtct gacccggaag cagaaatccg tcaactgaac agccgtcgcg
    18601 tcgaactgga gcgggcgtta agtaatcatg aaaatgataa ccagcagcag cgtattcagt
    18661 ttgagcaggc gaaagagggc gttacggcgc tgaaccgcat tctgccgcgt ctcaacctat
    18721 tggctgatga cagcctggcg gatcgcgtcg atgaaatccg cgaacgtctg gatgaagccc
    18781 aggaagccgc gcgttttgtt cagcagtttg gcaatcaact ggcgaaactg gaaccgattg
    18841 tttcggtatt gcagagcgac ccggaacagt tcgaacagtt aaaagaagat tacgcgtact
    18901 ctcagcagat gcagcgcgat gcccgtcagc aggcgtttgc cctgacggaa gtggtgcagc
    18961 gtcgtgcgca ctttagctat tctgactcgg cagaaatgct tagcggtaac agcgatctca
    19021 acgaaaaact gcgtgaacgt ctggaacagg cggaagcgga gcgtacccgc gctcgcgaag
    19081 cgttgcgcgg tcacgcagcg cagttgagtc agtacaacca ggtgctggct tcgctgaaaa
    19141 gttcttacga caccaaaaaa gagctactca acgatctgca acgtgaattg caggatatcg
    19201 gcgtgcgtgc tgatagcggg gcagaagagc gggcgcgtat tcgccgtgac gagctgcatg
    19261 cgcaactgag caataaccgt tcacgccgca atcaactgga aaaagcgctt accttctgcg
    19321 aagcggagat ggacaacctg acccgcaaac tgcgcaagct ggagcgggat tactttgaga
    19381 tgcgcgagca ggtagtgacc gccaaagcgg gctggtgtgc ggtgatgcgc atggtgaaag
    19441 ataacggcgt tgagcgccgc ttacaccgtc gtgagctggc ttatctctcc gctgatgatt
    19501 tgcgttccat gtcggataag gcgttaggtg cgctgcgtct ggcggtggcg gataacgaac
    19561 atctgcgcga cgtgctgcgc atgtcggaag atccgaaacg tccggagcgt aaaattcagt
    19621 tcttcgtggc ggtttatcag catctgcgtg aacgtattcg tcaggatatt attcgtaccg
    19681 atgatccggt ggaagctatc gaacagatgg agattgaact tagccgtctg accgaagaat
    19741 taacctcccg tgaacagaaa ctggcgatca gttcccgcag cgtggcgaac atcattcgca
    19801 aaaccattca gcgcgagcag aaccgtatcc gtatgctcaa ccaggggttg cagaacgtat
    19861 cgtttggtca ggtgaacagc gtgcgtctca acgtgaacgt gcgtgaaacg cacgccatgc
    19921 tactggatgt gctctctgaa cagcacgagc agcatcagga tctgtttaac agcaaccgtt
    19981 tgaccttctc ggaagcgctg gcgaaactgt atcaacgtct taacccgcag attgatatgg
    20041 ggcagcgcac gccgcagacc atcggtgaag aactgctgga ttaccgcaac tatctggaaa
    20101 tggaagttga ggttaaccgt ggttccgatg gctggctgcg cgcagagtct ggtgcattgt
    20161 cgaccggtga ggcgattggt accggtatgt cgattctggt gatggtggta caaagctggg
    20221 aagatgaatc tcgccgcctg cgcggtaaag atatctctcc ttgccgcctg ctgttcctcg
    20281 atgaagcagc gcgactggat gctcgttcta tcgccacgct gtttgaattg tgtgagcgtt
    20341 tgcaaatgca actcatcatc gcagcgccgg aaaatatcag cccggagaaa ggcaccacct
    20401 ataaactggt gcgtaaagtc ttccagaata ccgaacacgt tcatgtcgtc ggcctgcgag
    20461 gatttgcgcc gcaactccct gaaacgcttc caggaactga cgaagcgcct tctcaggcga
    20521 gttaaaatta aggcggcagc aatgccgcct tttctttttc cgaaaactcc gtttctgcac
    20581 taaaaaagtg gcacattacg gcgcgctttt ctttaaactt ctttacatta ggttatgtaa
    20641 aaacgtatcg gcgtttatat actgaagata agcctgatga gtaacaggct tgctcgtcat
    20701 actttcgtga gtattggcgt tgtacaggca agtcgtaaaa taacagcctg gctattcaga
    20761 gtatgataaa aacagggggc aagggatgtt gcttaatatg atgtgtggtc gtcggctgtc
    20821 ggcaatcagt ttgtgcctgg ctgtaacatt cgctccactg ttcaatgcgc aggccgatga
    20881 gcctgaagta atccctggcg acagcccggt ggctgtcagt gaacagggcg aggcactgcc
    20941 gcaggcgcaa gccacggcaa taatggcggg gatccagcca ttgcctgaag gtgcggcaga
    21001 aaaagcccgc acgcaaatcg aatctcaatt acccgcaggt tacaagccgg tttatcttaa
    21061 ccagcttcaa ctgttgtatg ccgcacgcga tatgcaaccc atgtgggaaa accgtgatgc
    21121 tgttaaagcc ttccagcaac agctggcaga ggtggcgatt gccggtttcc agccgcagtt
    21181 taataaatgg gtagagttac tgaccgatcc tggtgttaac gggatggcac gcgacgtggt
    21241 gctctctgat gcgatgatgg gctatctcca tttcattgca aatattccgg tcaaaggcac
    21301 tcgctggcta tatagcagta aaccttatgc acttgcaacg ccgccgctct cggtgattaa
    21361 ccaatggcag ctggcgctgg ataaaggtca attgcctacg tttgttgcag gactggcacc
    21421 gcagcatccg caatatgcgg cgatgcatga atcgttactg gccttactca gtgacaccaa
    21481 accgtggccc caactgaccg gcaaagcaac gttgcgccca gggcagtgga gtaacgacgt
    21541 accggcgttg cgcgaaatat tgcaacgcac aggcatgttg gacggggggc cgaaaattac
    21601 tctacctggc gatgacacgc caactgacgc ggtagtcagc ccatccgctg ttactgttga
    21661 aacagcagaa actaagccga tggataagca aacgacgtct cgtagtaaac ctgcgcctgc
    21721 cgttcgcgcc gcctacgata atgaactggt ggaagccgtt aaacgttttc aggcatggca
    21781 aggattgggg gcagatggtg ctattggccc ggcaacacgt gactggttaa acgtaacgcc
    21841 cgcccagcgt gctggcgtgt tggctctcaa catccagcga ttgcgcttgc tgccaacaga
    21901 gctttctacc gggatcatgg ttaacattcc ggcctattcg ctggtctact atcagaacgg
    21961 caatcaggtg ctggattcgc gagtcattgt cggtcgcccc gatcgcaaaa cgccgatgat
    22021 gagcagtgcc cttaacaacg tagtggtaaa cccgccgtgg aacgtacctc caactctggc
    22081 acgcaaagat attctgccaa aagtgcgcaa cgatccggga tatctcgaaa gccatggcta
    22141 tacggtgatg cgcggctgga acagcagaga agcgattgac ccatggcagg ttgactggtc
    22201 tacaatcacg gcctcgaatt taccgttccg cttccagcag gctccaggcc cacggaactc
    22261 gctggggcgc tataaattca atatgccgag ttcagaggcc atttatttgc atgacacgcc
    22321 gaaccacaat ctgttcaagc gtgatacacg cgcattgagc tcaggctgtg tacgagtgaa
    22381 taaagcttcc gatctggcga atatgctgtt gcaggatgca ggctggaatg acaaacgtat
    22441 ttctgatgcg ctgaagcagg gtgatacacg ttacgtcaat attcggcagt cgattccggt
    22501 gaatctctac tacctgacgg cctttgttgg tgcagatggt cgtacccagt atcgtacaga
    22561 tatttacaat tatgatctgc ctgcgcgatc cagctcgcaa atcgtatcga aagcggaaca
    22621 attaatcagg taaatgaagt agttcgggga aatcagttgt cgtaataatt agcatgattg
    22681 ggggcgattc tctgcagccc ccgtcactgc tggggttgag tcatcttgac gtctgcttta
    22741 cgggcggtta aggtgcctct tgtgcgccag aagtgcatat aaacgataac attgacctgt
    22801 agacttgatt atcatggaca aattcgacgc taatcgccgc aaattgctgg cgcttggtgg
    22861 cgttgcactc ggtgccgcca tcctgccgac ccctgcgttt gcaacactct ctaccccacg
    22921 cccgcgcatt ttgacactca ataatcttca taccggagag tcaatcaaag cggagttttt
    22981 cgatggcaga ggctatattc aggaagaatt ggcaaaactt aaccattttt tccgcgatta
    23041 ccgcgcgaac aaaataaagt ccatcgaccc aggattattc gaccagttgt atcgcctgca
    23101 agggttgtta ggcacgcgca aaccggtgca actcatttcc ggttatcgtt ctattgatac
    23161 caacaatgaa ctacgcgccc gcagccgtgg agtagcgaag aaaagctatc acactaaagg
    23221 ccaggcgatg gatttccata ttgaaggtat cgcgttaagc aatattcgca aagccgcgtt
    23281 atctatgcgc gcaggtggtg taggatatta tccacgtagt aactttgtgc atattgatac
    23341 cgggccagca cggcactggt agtaattgct taacgaaaca ggggcagtat gaactatcgt
    23401 attattccgg tcaccgcatt ctcccagaac tgttcattaa tctggtgtga acaaacccgt
    23461 ctggccgcac tggtcgatcc tggcggcgat gcggaaaaaa tcaaacagga agttgatgac
    23521 agcggcctga cactgatgca gatcctgctg acgcatggtc atctggacca cgttggcgca
    23581 gcggcggaac tggcgcaaca ttacggcgtg ccggttttcg gcccggaaaa agaagatgag
    23641 ttctggctgc aaggcttgcc tgcgcaaagt cgtatgtttg gtctggaaga gtgccagccg
    23701 ctgacgccag atcgttggct gaacgaaggc gataccatca gcatagggaa tgtgacttta
    23761 caggtgttac attgccctgg gcatacgccg ggtcatgtcg tgttttttga tgatcgggca
    23821 aagctgctga tttctggcga tgttattttc aaaggcggag tagggcgcag tgacttcccg
    23881 cgtggcgatc ataatcaact gatttcttca atcaaagata aattgctgcc actgggggat
    23941 gacgtgatat ttattccggg tcacggacca ttatccacac ttggttatga acgcctgcat
    24001 aatcccttcc tgcaagacga aatgcccgtc tggtaaggca cataaaaaag cccgctttta
    24061 atgcgggcct ggatttctgg caaagtgcgc tttgtttatg ccggatgcgg cacgagcgcc
    24121 ttatccggcc tacaaaatcg tgcaaattca aaatattgca ggggacgcgt aggcctgata
    24181 agcgtagcgc atcaggcaat gttgcgtttg tcatcagtct cagcccgctt ttcagcgggc
    24241 ttcattgttt ttaatgctta cagcactgcc acaatcgctt cgcacagcgg agccatgtta
    24301 tctggtgtca tcccggccac atttacgcga ccagaagcaa ccgcatatac gccaaactct
    24361 tcgcgcagac gcagcacttg ttcttttgtc aggccactga aggagaacat gccgttctgt
    24421 ttgatgataa agctgaagtc gcggtttgcg cctttttcct gcagcgtatt gacgaacaac
    24481 tgacgcatac gctgaatacg ctggcgcata tcagtcagct cttgttccca aatcgcacgt
    24541 aacgcatcgt tgctcaggat ggtggcaaca acagaagcgc cgtgtgctgg tgggttagag
    24601 tagttagcgc gaatcgccgc tttcatttgg ctgaatgcgc gatcaacggt ttcactgtcg
    24661 gcagcaacca gagtacaagc gccaacacgc tcgttgtaca ggccaaagtt tttagagtag
    24721 gaactggcaa caatcagctc tttatgcata gccgcgaaag cgcgcagtcc ttcagcatct
    24781 tcttccagac cacgggcaaa accctggtaa gcgaagtcaa acagcggtaa ccagcctttc
    24841 tcaacggaga gttgtgccag tgtttgccat tgttccagcg tagggtcgat accggttggg
    24901 ttatggcagc agccatggaa cagcactacg tcgccagcct gagcttcatt caggctgtta
    24961 atcagtgcat cgaagtcaag agtgtgattt tccgcatcat aataagcgta ttcacgaact
    25021 tccagacctg cagagttaaa gacgctctta tggttcggcc agcttgggtt gctcacccac
    25081 acacgcttaa cgctggtatt ttttgccagg aaatcggcag ccacgcgtag tgcgccagtg
    25141 ccccccggag tctgtgccgt gcgagcacgt ttgtcattga tcagggcgct acctttacca
    25201 aacagcagtt cctgagtgca gcgaccaaat tcagggatgc cgtcaatgcc gaggtaattt
    25261 ttggtggttt cattttcgag cagatactgt tcagcctttt tcacgctggt cagtaccggg
    25321 gttttgcccg tctcatcttt atagacacca atcccgaggt taattttgcc gggacgttca
    25381 tcggcacgaa acagatcggc caggcccaga atcgggtcgg caggagcggc ggtaatgttc
    25441 tcaaacatga cgaggttcca ttatggttac agaagggaag tccgctatca gggtaacggg
    25501 agatttacaa aattccaact attactgatg aaaactcagg ctgtttttgc aagacgtgag
    25561 attgctctgg aaggtataaa aaaaacagga ccaaagtcct gttttttcgg catttaacaa
    25621 agaggtgtgc tattagaact ggtaaacgat acccacagca acggtgtcgt ctgaacctac
    25681 gcccagtttg ttgtcagaat cgatctggtt gatgatgtag tcaacatagg tggacatgtt
    25741 tttgttgaag tagtaggttg cgcccacttc aaagtagttc accagatcaa catcaccgat
    25801 accttctacg tctttcgctt tagatttggt gtaagcgatg gacggacgca gaccgaaatc
    25861 gaactggtat tgcgcaacta acagaacgtc ttgcgttttg ttggcgaagc cgctggtgtt
    25921 tgtaaattta ttagtgatcg gcgtagcgtt acgggtttca ccgtagttcg ctgccaggta
    25981 gatgttgttc gcgtcgtact tcagaccagt agcccactgt tcagcttttt taccgttgcc
    26041 aagaggttga gcttcttgca agttggtacg gtcagctgca ccataagcac caacgatacc
    26101 aaagccttcg tattcgtagc tgatagaacc gccaacaccg tcgccgttag agcggcgtgc
    26161 agtgtcacgc tcgtttttac ccaggtactg aacagcgaag ttcaggccat caaccagacc
    26221 aaagaagttg gagttacgat aggtagcaac gccgccaaca cgaccaacga agaagtcatc
    26281 gctgtatgca gtatcaccac caaattctgg cagcatatcg gtgtaaccca gtgcatcata
    26341 aaccacaccg tagttacggc cgtaatcgaa agaaccaatg tcagcgtatt taagacccgc
    26401 gaatgccaga cgcgttttgt taccagtttg agcgtcagcg ccttcagagt tgttaccctg
    26461 gaagttatat tcccactgac cataaccggt cagatcggaa ttgatttgtg tttccccttt
    26521 aaaaccaaga cgggcataag ttttgtcgcc gtttccgccg taactgttta caccattgtc
    26581 tttagaaaaa taatgcagac cgacagcttt accgtacaga tctactttgt tgccatcttt
    26641 gttatagatt tctgcagcgt ttgcagtacc tgctactaac agagcaggga cgatcactgc
    26701 cagaatattg cgcttcatca ttatttatta ccctcatggt tttttttatg acacctgcca
    26761 ctgccgtcaa taagttctgt caataaaaat ttacggaact attgatgaga gtttggtgtc
    26821 tttatgtgtc tgcaggcatc tttccattca aactaacgtt tcgctaccgt gaaagtgcta
    26881 caaagataaa gatttggttt caaaaagaaa aaatatgtaa ccaaaagtaa aatttaagga
    26941 actttgtgaa cacc
//
LOCUS       DAJOSY010000060        26407 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJOSY010000060 DAJOSY010000000
VERSION     DAJOSY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.060"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            5..1474
                     /gene="gspA"
                     /locus_tag="OHN27_002602"
     CDS             5..1474
                     /gene="gspA"
                     /locus_tag="OHN27_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCQ2287015.1"
                     /translation="MSTRREVILSWLCEKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSIGRLTEH
                     ELKVSILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTATLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTHKWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1476..1895
                     /gene="gspB"
                     /locus_tag="OHN27_002603"
     CDS             1476..1895
                     /gene="gspB"
                     /locus_tag="OHN27_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCQ2287016.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEENTVEESEEKAGLRERVKNALNELER"
     gene            2133..2444
                     /gene="rpsJ"
                     /locus_tag="OHN27_002604"
     CDS             2133..2444
                     /gene="rpsJ"
                     /locus_tag="OHN27_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCQ2287017.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2477..3106
                     /gene="rplC"
                     /locus_tag="OHN27_002605"
     CDS             2477..3106
                     /gene="rplC"
                     /locus_tag="OHN27_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCQ2287018.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3117..3722
                     /gene="rplD"
                     /locus_tag="OHN27_002606"
     CDS             3117..3722
                     /gene="rplD"
                     /locus_tag="OHN27_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCQ2287019.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVIMTADAVKQVEEMLA"
     gene            3719..4021
                     /gene="rplW"
                     /locus_tag="OHN27_002607"
     CDS             3719..4021
                     /gene="rplW"
                     /locus_tag="OHN27_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCQ2287020.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4039..4860
                     /gene="rplB"
                     /locus_tag="OHN27_002608"
     CDS             4039..4860
                     /gene="rplB"
                     /locus_tag="OHN27_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCQ2287021.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4877..5155
                     /gene="rpsS"
                     /locus_tag="OHN27_002609"
     CDS             4877..5155
                     /gene="rpsS"
                     /locus_tag="OHN27_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCQ2287022.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5170..5502
                     /gene="rplV"
                     /locus_tag="OHN27_002610"
     CDS             5170..5502
                     /gene="rplV"
                     /locus_tag="OHN27_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCQ2287023.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5520..6221
                     /gene="rpsC"
                     /locus_tag="OHN27_002611"
     CDS             5520..6221
                     /gene="rpsC"
                     /locus_tag="OHN27_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCQ2287024.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6234..6644
                     /gene="rplP"
                     /locus_tag="OHN27_002612"
     CDS             6234..6644
                     /gene="rplP"
                     /locus_tag="OHN27_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCQ2287025.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6644..6835
                     /gene="rpmC"
                     /locus_tag="OHN27_002613"
     CDS             6644..6835
                     /gene="rpmC"
                     /locus_tag="OHN27_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCQ2287026.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6835..7089
                     /gene="rpsQ"
                     /locus_tag="OHN27_002614"
     CDS             6835..7089
                     /gene="rpsQ"
                     /locus_tag="OHN27_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCQ2287027.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7254..7625
                     /gene="rplN"
                     /locus_tag="OHN27_002615"
     CDS             7254..7625
                     /gene="rplN"
                     /locus_tag="OHN27_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCQ2287028.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7636..7950
                     /gene="rplX"
                     /locus_tag="OHN27_002616"
     CDS             7636..7950
                     /gene="rplX"
                     /locus_tag="OHN27_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCQ2287029.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7965..8504
                     /gene="rplE"
                     /locus_tag="OHN27_002617"
     CDS             7965..8504
                     /gene="rplE"
                     /locus_tag="OHN27_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCQ2287030.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8519..8824
                     /gene="rpsN"
                     /locus_tag="OHN27_002618"
     CDS             8519..8824
                     /gene="rpsN"
                     /locus_tag="OHN27_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCQ2287031.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8858..9250
                     /gene="rpsH"
                     /locus_tag="OHN27_002619"
     CDS             8858..9250
                     /gene="rpsH"
                     /locus_tag="OHN27_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCQ2287032.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9263..9796
                     /gene="rplF"
                     /locus_tag="OHN27_002620"
     CDS             9263..9796
                     /gene="rplF"
                     /locus_tag="OHN27_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCQ2287033.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9806..10159
                     /gene="rplR"
                     /locus_tag="OHN27_002621"
     CDS             9806..10159
                     /gene="rplR"
                     /locus_tag="OHN27_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCQ2287034.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10174..10677
                     /gene="rpsE"
                     /locus_tag="OHN27_002622"
     CDS             10174..10677
                     /gene="rpsE"
                     /locus_tag="OHN27_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCQ2287035.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10681..10860
                     /gene="rpmD"
                     /locus_tag="OHN27_002623"
     CDS             10681..10860
                     /gene="rpmD"
                     /locus_tag="OHN27_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCQ2287036.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10864..11298
                     /gene="rplO"
                     /locus_tag="OHN27_002624"
     CDS             10864..11298
                     /gene="rplO"
                     /locus_tag="OHN27_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCQ2287037.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11306..12637
                     /gene="secY"
                     /locus_tag="OHN27_002625"
     CDS             11306..12637
                     /gene="secY"
                     /locus_tag="OHN27_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCQ2287038.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12669..12785
                     /gene="rpmJ"
                     /locus_tag="OHN27_002626"
     CDS             12669..12785
                     /gene="rpmJ"
                     /locus_tag="OHN27_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCQ2287039.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12932..13288
                     /gene="rpsM"
                     /locus_tag="OHN27_002627"
     CDS             12932..13288
                     /gene="rpsM"
                     /locus_tag="OHN27_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCQ2287040.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13305..13694
                     /gene="rpsK"
                     /locus_tag="OHN27_002628"
     CDS             13305..13694
                     /gene="rpsK"
                     /locus_tag="OHN27_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCQ2287041.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13728..14348
                     /gene="rpsD"
                     /locus_tag="OHN27_002629"
     CDS             13728..14348
                     /gene="rpsD"
                     /locus_tag="OHN27_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCQ2287042.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14374..15363
                     /gene="rpoA"
                     /locus_tag="OHN27_002630"
     CDS             14374..15363
                     /gene="rpoA"
                     /locus_tag="OHN27_002630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCQ2287043.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15404..15787
                     /gene="rplQ"
                     /locus_tag="OHN27_002631"
     CDS             15404..15787
                     /gene="rplQ"
                     /locus_tag="OHN27_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCQ2287044.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15894..16262
                     /locus_tag="OHN27_002632"
     CDS             15894..16262
                     /locus_tag="OHN27_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCQ2287045.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16273..16698
                     /gene="zntR"
                     /locus_tag="OHN27_002633"
     CDS             16273..16698
                     /gene="zntR"
                     /locus_tag="OHN27_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCQ2287046.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16754..16972
                     /gene="arfA"
                     /locus_tag="OHN27_002634"
     CDS             16754..16972
                     /gene="arfA"
                     /locus_tag="OHN27_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCQ2287047.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16969..17379)
                     /gene="mscL"
                     /locus_tag="OHN27_002635"
     CDS             complement(16969..17379)
                     /gene="mscL"
                     /locus_tag="OHN27_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCQ2287048.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17509..18885)
                     /gene="trkA"
                     /locus_tag="OHN27_002636"
     CDS             complement(17509..18885)
                     /gene="trkA"
                     /locus_tag="OHN27_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCQ2287049.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18907..20196)
                     /gene="rsmB"
                     /locus_tag="OHN27_002637"
     CDS             complement(18907..20196)
                     /gene="rsmB"
                     /locus_tag="OHN27_002637"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCQ2287050.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKTGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20242..21189)
                     /gene="fmt"
                     /locus_tag="OHN27_002638"
     CDS             complement(20242..21189)
                     /gene="fmt"
                     /locus_tag="OHN27_002638"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCQ2287051.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVAELQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21204..21713)
                     /gene="def"
                     /locus_tag="OHN27_002639"
     CDS             complement(21204..21713)
                     /gene="def"
                     /locus_tag="OHN27_002639"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCQ2287052.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKDRA"
     gene            21843..22967
                     /gene="dprA"
                     /locus_tag="OHN27_002640"
     CDS             21843..22967
                     /gene="dprA"
                     /locus_tag="OHN27_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCQ2287053.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22939..23412
                     /locus_tag="OHN27_002641"
     CDS             22939..23412
                     /locus_tag="OHN27_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCQ2287054.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23441..23983
                     /locus_tag="OHN27_002642"
     CDS             23441..23983
                     /locus_tag="OHN27_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCQ2287055.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23988..24560
                     /gene="tsaC"
                     /locus_tag="OHN27_002643"
     CDS             23988..24560
                     /gene="tsaC"
                     /locus_tag="OHN27_002643"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCQ2287056.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24565..25383
                     /gene="aroE"
                     /locus_tag="OHN27_002644"
     CDS             24565..25383
                     /gene="aroE"
                     /locus_tag="OHN27_002644"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCQ2287057.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALSMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELST"
     gene            25380..25637
                     /locus_tag="OHN27_002645"
     CDS             25380..25637
                     /locus_tag="OHN27_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCQ2287058.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25613..26167)
                     /locus_tag="OHN27_002646"
     CDS             complement(25613..26167)
                     /locus_tag="OHN27_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCQ2287059.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6729 a         6235 c         6910 g         6533 t
ORIGIN      
        1 ttctatgtct acgagaagag aagttattct ttcctggttg tgtgagaaac gtcaaacctg
       61 gcgtctatgc tatttgttgg gtgaggctgg aagtggaaaa acctggctgg cgcagcaact
      121 gcaaaaagat aaacatcgcc gtgtgattac tttaagcctc gttgtttcct ggcaaggtaa
      181 ggccgcatgg atcgttaccg acgataacgc ggctgaacag ggctgccgtg acagcgcctg
      241 gacgcgagat gagatggcgg ggcaattact gcatgcgctt catcgaactg atagtcgctg
      301 ccctcttatt attattgaaa atgctcacct gaatcaccgc cgcatacttg atgatctcca
      361 acgcgcaata tctcttattc ctgacggtca atttttgctg atcggcagac ccgatcgaaa
      421 agtcgaacgt gattttaaaa aacagggcat tgaacttgtc tcgataggac gtctgacgga
      481 gcacgaactt aaggtaagca tccttgaagg tcagaatatt gaccaacccg atctcctttt
      541 aaccgccaga gttctgaaac ggatagcttt attatgtcgg ggcgatcgca gaaagctggc
      601 gcttgccggc gaaacaattc gcttattaca gcaagccgag cagaccagcg ttttcacagc
      661 aaaacaatgg cgaatgattt accgcatatt gggtgataac cgcccccgga aaatgcagct
      721 tgccgtggtg atgagtggaa ccattatcgc tcttacctgt ggttggctac tgctgtcctc
      781 ttttacagct acgttacccg ttcctgcgtg gctgataccc gtaacgccag tagttaaaca
      841 agatatgact aaggatattg ctcatgtggt gatgcgcgat agtgaggcgc taagcgtgtt
      901 atacggcgta tgggggtacg aggtcccagc tgattcggcc tggtgcgatc aggcagtaag
      961 agcggggctg gcatgtaaaa gtgggaatgc ttctttacaa acattggtag atcagaattt
     1021 gccctggatc gcatcgctta aagtcgggga caaaaaattg cctgttgtgg tcgttcgcgt
     1081 cggtgaggct agcgttgatg tcctcgtagg tcagcaaacg tggacgctga cacataaatg
     1141 gtttgagtcg gtatggacgg gtgattatct tctgttgtgg aaaatgtcgc cggaagggga
     1201 gagtaccatt acgcgtgata gcagcgagga agagattctc tggctggaaa cgatgttaaa
     1261 ccgcgcattg catatttcga cagaaccttc ggcggagtgg cgtcccttgt tggtagaaaa
     1321 aataaagcaa tttcaaaaaa gccaccactt gaaaactgac ggtgttgttg gcttttcaac
     1381 gttagtacat ctctggcagg tggcagggga gagtgcctac ttatatcggg atgaagcaaa
     1441 tatttccccg gaaacaacag tgaaggggaa atgacatgtt tgaattctat atcgccgcgc
     1501 gtgagcagaa agaaacaggt caccctggta tcttctcccg acaaaaacac agcaccataa
     1561 tatatgtaat ttgtttatta ctgatttgcc tgtggtttgc aggaatggtg ctggttggtg
     1621 ggtatgccag gcagctatgg gtgctttgga tagtaaaagc cgaagtcact gtagaggctg
     1681 aaacgcccgc ttttaaacaa tcaacacaac actatttctt caaaaaacag ccactccccg
     1741 tcgtcgaatc cgtcgaggag gaagatgacc caggcgtcgc ggttgagaat gccccctctt
     1801 cttctgaaga cgaagaaaat actgtggaag aaagcgaaga gaaagccggt ttaagagagc
     1861 gtgtcaaaaa tgcactgaac gaacttgaga gataacccga aggctgttta ctttactagc
     1921 aatacgcttg cgttcggtgg ttaagtatgt ataatgcgcg ggcttgtcgt agttgacagc
     1981 aggttcaatc tgaacctcag ttagccgaat ttggctacct aacaatgctc ccaatcgggg
     2041 agctacgtaa gaacggttac actctcccat caatcgtaat gggtctgagg agtaatcatt
     2101 ttcgtttata aaataattgg agctctggtc tcatgcagaa ccaaagaatc cgtatccgcc
     2161 tgaaagcgtt tgatcatcgt ctgatcgatc aagcaaccgc ggaaatcgtc gagactgcca
     2221 agcgcactgg tgcgcaggtc cgtggtccga tcccgctgcc gacacgcaaa gagcgcttca
     2281 ctgttctgat ctccccgcac gtcaacaaag acgcgcgcga tcagtacgaa atccgtactc
     2341 acttgcgtct ggttgacatc gttgagccaa ccgagaaaac cgttgatgct ctgatgcgtc
     2401 tggatctggc tgccggtgta gacgtgcaga tcagcctggg ttaatcaggt cattgagcga
     2461 ttgagaggtt gaaacaatga ttggtttagt cggtaaaaaa gtgggtatga cccgtatctt
     2521 cacagaagac ggcgtttcta tcccagtaac cgtaatcgaa gttgaagcaa accgcgttac
     2581 tcaggttaaa gacctggcta acgatggcta ccgtgctatt caggtgacca ccggtgctaa
     2641 aaaagctaac cgtgtgacca agcctgaagc tggccacttc gctaaagctg gcgtagaagc
     2701 tggccgtggt ctgtgggaat tccgcctggc tgaaggcgaa gagttcactg taggtcagag
     2761 cattagcgtt gaactgtttg ctgacgttaa aaaagttgac gtaactggca cctctaaagg
     2821 taaaggtttc gcaggtaccg ttaagcgctg gaacttccgt acccaggacg ctactcacgg
     2881 taactccttg tctcaccgcg ttccgggttc tatcggtcag aaccagactc cgggcaaagt
     2941 gttcaaaggc aagaaaatgg caggtcagat gggtaacgaa cgtgtaaccg ttcagagcct
     3001 tgacgtagta cgcgttgacg ctgagcgcaa cctgctgctg gttaaaggtg ctgtcccggg
     3061 tgcaaccggt agcgacctga tcgttaaacc agctgtgaag gcgtaaggag atagcaatgg
     3121 aattagtatt gaaagacgcg cagagcgcgc tgactgtttc cgaaactacc ttcggtcgtg
     3181 atttcaacga agcgctggtt caccaggttg ttgttgctta tgcagctggt gctcgtcagg
     3241 gtactcgtgc tcagaagact cgtgctgaag taactggttc cggtaaaaaa ccgtggcgcc
     3301 agaaaggcac cggccgtgcg cgttctggtt ctatcaagag cccgatctgg cgttctggtg
     3361 gcgtgacctt tgctgctcgt ccgcaggacc acagtcaaaa agttaacaag aagatgtacc
     3421 gcggcgcgct gaaaagcatc ctgtccgaac tggtacgtca ggatcgtctg atcgttgtcg
     3481 agaagttctc tgtagaagcg ccgaaaacta agctgctggc acagaaactg aaagacatgg
     3541 ctctggaaga tgtgctgatc atcaccggtg agctggacga aaacctgttc ctggctgcgc
     3601 gcaacctgca caaggttgac gtacgcgatg caactggtat cgacccggtt agcctgatcg
     3661 ccttcgacaa agtcataatg actgctgatg ctgttaagca agttgaggag atgctggcat
     3721 gattcgtgaa gaacgtctgc tgaaggtgct gcgtgcaccg cacgtttctg aaaaagcgtc
     3781 tactgcgatg gaaaaatcca acaccatcgt actcaaagtt gctaaagacg cgaccaaagc
     3841 agaaatcaaa gctgctgtgc agaaactgtt tgaagtcgaa gtcgaagtcg ttaacaccct
     3901 ggtagttaaa gggaaagtta aacgtcacgg acagcgtatc ggtcgtcgta gcgactggaa
     3961 aaaagcttac gtcaccctga aagaaggcca gaatctggac ttcgttggcg gcgctgagta
     4021 agtcggagga gtaatacaat ggcagttgtt aaatgtaaac cgacatctcc gggtcgtcgc
     4081 cacgtagtta aagtggttaa ccctgagctg cacaagggca aaccttttgc tccgttgctg
     4141 gaaaaaaaca gcaaatccgg tggtcgtaac aacaatggcc gtatcaccac tcgtcatatc
     4201 ggtggtggcc acaagcaggc ttaccgtatt gttgacttca aacgcaacaa agacggtatc
     4261 ccggcagttg ttgaacgtct tgagtacgat ccgaaccgtt ccgcgaacat cgcgctggtt
     4321 ctgtacaaag acggtgaacg ccgttacatc ctggccccta aaggcctgaa agctggcgac
     4381 cagattcagt ctggcgttga tgctgcaatc aaaccaggta acaccctgcc gatgcgcaac
     4441 atcccggttg gttctactgt tcataacgta gaaatgaaac caggtaaagg cggtcagctg
     4501 gcacgttccg ctggtactta cgttcagatc gttgctcgtg atggtgctta tgtcaccctg
     4561 cgtctgcgtt ctggtgaaat gcgtaaagta gaagcagact gccgtgcaac tctgggcgaa
     4621 gttggcaatg ctgagcatat gctgcgcgtt ctgggtaaag caggtgctgc acgctggcgt
     4681 ggtgttcgtc cgaccgttcg cggtaccgcg atgaacccgg tagaccaccc acatggtggt
     4741 ggtgaaggtc gtaactttgg taagcacccg gtaactccgt ggggcgttca gaccaaaggt
     4801 aagaagaccc gcagcaacaa gcgtactgat aaattcatcg tacgtcgccg tagcaaataa
     4861 ttttagagga taagccatgc cacgttctct caagaaaggt ccttttattg acctgcactt
     4921 gctgaagaag gtagagaaag cggtggaaag cggagacaag aagcccctgc gcacttggtc
     4981 ccgtcgttca acgatctttc ctaacatgat cggtttgacc atcgctgtcc ataatggtcg
     5041 tcagcacgtt ccggtatttg taaccgacga aatggttggt cacaaactgg gtgaattcgc
     5101 accgactcgt acttatcgcg gccacgctgc tgataaaaaa gcgaagaaga aataaggtag
     5161 gaggaagaga tggaaactat cgctaaacat cgccatgctc gttcttctgc tcagaaggtt
     5221 cgccttgttg ctgacctgat tcgcggtaag aaagtgtcgc aggctctgga tattttgacc
     5281 tacaccaaca agaaagcggc tgtactggtc aagaaagttc tggaatctgc cattgctaac
     5341 gctgaacaca acgatggcgc tgacattgac gatctgaaag ttacgaaaat tttcgtagac
     5401 gaaggcccga gcatgaagcg cattatgccg cgtgcaaaag gtcgtgcaga tcgcatcctg
     5461 aagcgcacca gccacatcac tgtggttgtg tccgatcgct gagactctgg agactagcaa
     5521 tgggtcagaa agtacatcct aatggtattc gcctgggtat tgtaaaacca tggaactcta
     5581 cctggtttgc gaacaccaaa gaattcgctg acaacctgga cagcgatttt aaagtacgtc
     5641 agtacctgac taaggaactg gctaaagcgt ccgtatctcg tatcgttatc gagcgtccgg
     5701 ctaagagcat ccgtgtaacc attcacactg ctcgcccggg tatcgttatc ggtaaaaaag
     5761 gtgaagacgt agaaaaactg cgtaaggtcg tagcggacat cgctggcgtt cctgcacaga
     5821 tcaacatcgc cgaagttcgt aagcctgaac tggacgcaaa actggttgct gacagcatca
     5881 cttctcagct ggaacgtcgc gttatgttcc gtcgtgctat gaagcgtgct gtacagaacg
     5941 caatgcgtct gggcgctaaa ggtattaaag ttgaagttag cggccgtctg ggcggcgcgg
     6001 aaatcgcacg taccgaatgg taccgcgaag gtcgcgtacc gctgcacact ctgcgtgctg
     6061 acatcgacta caacacctct gaagcgcaca ccacttacgg tgtaatcggc gttaaagtgt
     6121 ggatcttcaa aggcgagatc ctgggtggta tggctgctgt tgaacaaccg gaaaaaccgg
     6181 ctgctcagcc taaaaagcag cagcgtaaag gccgtaaata aggagcgtcg ctgatgttac
     6241 aaccaaagcg tacaaaattc cgtaaaatgc acaaaggccg taaccgcggt ctggcgcagg
     6301 gtacggatgt tagcttcggc agcttcggtc tgaaagctgt tggccgtggt cgtctgactg
     6361 cccgtcagat cgaagcagca cgtcgtgcta tgacccgtgc agttaagcgt caaggtaaga
     6421 tctggatccg tgtgttcccg gacaaaccga tcactgaaaa gccgctggca gtgcgtatgg
     6481 gtaaaggtaa aggtaacgtg gagtattggg ttgccttgat tcagccgggt aaagtcctgt
     6541 atgaaatgga cggtgttccg gaagagctgg cccgtgaagc attcaagctg gcagcagcga
     6601 aactgccgat taaaaccacc tttgtaacta agacggtgat gtaatgaaag caaaagagct
     6661 gcgtgagaag agcgttgaag agctgaacac cgagctgctg aacctgctgc gtgagcagtt
     6721 caacctgcgt atgcaggctg caagtggcca gctgcaacag tctcacctgt tgaagcaagt
     6781 gcgtcgcgat gtcgcacgcg ttaagacttt actgaacgag aaggcgggtg cgtaatgacc
     6841 gataaaatcc gtactctgca aggtcgcgtt gttagcgaca aaatggagaa atccattgtt
     6901 gttgctatcg aacgttttgt gaaacacccg atctacggta aattcatcaa gcgtacgacc
     6961 aaactgcacg tacatgacga gaacaacgaa tgcggtatcg gtgacgtggt tgaaatccgc
     7021 gaatgccgtc cgctgtccaa gactaaatcc tggacgctgg ttcgcgttgt agagaaagcg
     7081 gttctgtaat acagtacact ctctcaatac gaataaacgg ctcagaaatg agccgtttat
     7141 tttttctacc catatccttg aagcggtgtt ataatgccgc gccctcgata tggggatttt
     7201 taacgacctg attttcgggt ctcagtagta gttgacatta gcggagcact aaaatgatcc
     7261 aagaacagac tatgctgaac gtcgccgaca actccggtgc acgtcgcgta atgtgtatca
     7321 aggttctggg tggctcgcac cgtcgctacg caggcgtagg cgacatcatc aagatcacca
     7381 tcaaagaagc aattccgcgt ggtaaggtca aaaaaggtga tgtgctgaag gcggtagtgg
     7441 tgcgcaccaa gaagggtgtt cgtcgcccgg acggttctgt cattcgcttc gatggtaatg
     7501 cttgtgttct tctgaacaac aacagcgagc agcctatcgg tacgcgtatt tttgggccgg
     7561 taactcgtga gcttcgtagt gagaagttca tgaaaattat ctctctggca ccagaagtac
     7621 tctaaggagc gaatcatggc agcgaaaatc cgtcgtgatg acgaagttat cgtgttaacc
     7681 ggtaaagata aaggtaaacg cggtaaagtt aagaatgtcc tgtcttccgg caaggtcatt
     7741 gttgaaggta tcaacctggt taagaaacat cagaagccgg ttccggccct gaaccaaccg
     7801 ggtggcatcg ttgaaaaaga agccgctatt caggtttcca acgtagcaat cttcaatgcg
     7861 gcaaccggca aggctgaccg tgtaggcttt agattcgaag acggtaaaaa agtccgtttc
     7921 ttcaagtcta acagcgaaac tatcaagtaa tttggagtag tacgatggcg aaactgcatg
     7981 attactacaa agacgaagta gttaaaaaac tcatgactga gtttaactac aattctgtca
     8041 tgcaagtccc tcgggtcgag aagatcaccc tgaacatggg tgttggtgaa gcgatcgctg
     8101 acaaaaaact gctggataac gcagcagcag acctggcagc aatctccggt caaaaaccgc
     8161 tgatcaccaa agcacgcaaa tctgttgcag gcttcaaaat ccgtcagggc tatccgatcg
     8221 gctgtaaagt aactctgcgt ggcgaacgca tgtgggagtt ctttgagcgc ctgatcacta
     8281 ttgctgtacc tcgtatccgt gacttccgtg gcctgtccgc taagtctttc gacggtcgtg
     8341 gtaactacag catgggtgtc cgtgagcaga tcatcttccc agaaatcgac tacgataaag
     8401 tcgaccgcgt tcgtggtttg gatattacca ttaccactac tgcgaaatct gacgaagaag
     8461 gccgcgctct gctggctgcc tttgacttcc cgttccgcaa gtaaggtagg gttactaaat
     8521 ggctaagcaa tcaatgaaag cacgcgaagt aaaacgcgta gctttagctg ataaatactt
     8581 cgcgaaacgc gctgaactga aagcgatcat ctctgatgtg aacgcttccg acgaagatcg
     8641 ttggaacgct gttctcaagc tgcagactct gccgcgtgat tccagcccgt ctcgtcagcg
     8701 taaccgctgc cgtcaaacag gtcgtccgca tggtttcctg cggaagttcg ggttgagccg
     8761 tattaaggtc cgtgaagccg ctatgcgcgg tgaaatcccg ggtctgaaaa aggctagctg
     8821 gtaattgtca ccaattgaat cacgggagta aagacagatg agcatgcaag atccgatcgc
     8881 ggatatgctg acccgtatcc gtaacggtca ggccgcgaac aaagctgcgg tcaccatgcc
     8941 ttcctccaag ctgaaagtgg caatcgccaa cgtgctgaag gaagaaggtt ttattgaaga
     9001 ttttaaagtt gaaggcgaca ccaagcctga actggaactt actctgaagt atttccaggg
     9061 caaagctgtt gtagaaagca ttcagcgtgt cagccgccca ggtctgcgca tctataaacg
     9121 taaagatgag ctgccgaaag ttatggcggg tctgggtatc gcagttgttt ctacctctaa
     9181 aggtgttatg actgatcgtg cagcgcgcca ggctggtctt ggtggcgaaa ttatctgcta
     9241 cgtagcctaa tcggaggaaa aaatgtctcg tgttgctaaa gcaccggtcg ttgttcctgc
     9301 cggcgttgac gtaaaaatca acggtcaggt tattacgatc aaaggtaaaa acggcgagct
     9361 gactcgtact ctcaacgatg ctgttgaagt taaacatgca gataataccc tgaccttcgg
     9421 tccgcgtgat ggttacgcag acggttgggc acaggctggt accgcgcgtg ccctgctgaa
     9481 ctcaatggtt atcggtgtta ccgaaggctt cactaagaag ctgcagctgg ttggtgtagg
     9541 ttaccgtgca gcggttaaag gcaatgtgat taacctgtct ctgggtttct ctcatcctgt
     9601 tgaccatcag ctgcctgcgg gtatcactgc tgaatgtccg actcagactg aaatcgtgct
     9661 gaaaggcgct gataagcagg tgatcggcca ggttgcagcg gatctgcgcg cctaccgtcg
     9721 tcctgagcct tataaaggca agggtgttcg ttacgccgac gaagtcgtgc gtaccaaaga
     9781 ggctaagaag aagtaaggta acactatgga taagaaatct gctcgtatcc gtcgtgcgac
     9841 ccgcgcacgc cgcaagctcc aggagctggg cgcaactcgc ctggtggtac atcgtacccc
     9901 gcgtcacatt tacgcacagg taattgcacc gaacggttct gaagttctgg tagctgcttc
     9961 tactgtagaa aaagctatcg ctgaacaact gaagtacacc ggtaacaaag acgcggctgc
    10021 agctgtgggt aaagctgtcg ctgaacgcgc tctggaaaaa ggcatcaaag atgtatcctt
    10081 tgaccgttcc gggttccaat atcatggtcg tgtccaggca ctggcagatg ctgcccgtga
    10141 agctggcctt cagttctaag gtagaggtgt aagatggctc acatcgaaaa acaagctggc
    10201 gaactgcagg aaaagctgat cgcggtaaac cgcgtatcta aaaccgttaa aggtggtcgt
    10261 attttctcct tcacagctct gactgtagtt ggcgatggta acggtcgcgt tggttttggt
    10321 tacggtaaag cgcgtgaagt tccagcagcg atccagaaag cgatggaaaa agcccgtcgc
    10381 aatatgatta acgtcgcgct gaataacggc actctgcaac accctgttaa aggtgttcac
    10441 acgggttctc gcgtattcat gcagccggct tccgaaggta ccggtatcat cgccggtggt
    10501 gcaatgcgcg ccgttctgga agtcgctggg gttcataacg ttctggctaa agcctatggt
    10561 tccaccaacc cgatcaacgt ggttcgtgca actattgatg gcctggaaaa tatgaattct
    10621 ccagaaatgg tcgctgccaa gcgtggtaaa tccgttgaag aaattctggg gaaataaacc
    10681 atggcaaaga ctattaaaat tactcaaacc cgcagtgcaa tcggtcgtct gccgaaacac
    10741 aaggcaacgc tgcttggcct gggtctgcgt cgtattggtc acaccgtaga gcgcgaggat
    10801 actcctgcta ttcgcggtat gatcaacgcg gtttccttca tggttaaagt tgaggagtaa
    10861 gagatgcgtt taaatactct gtctccggcc gaaggctcca aaaaggcggg taaacgcctg
    10921 ggtcgtggta tcggttctgg cctcggtaaa accggtggtc gtggtcacaa aggtcagaag
    10981 tctcgttctg gcggtggcgt acgtcgcggt ttcgagggtg gtcagatgcc tctgtaccgt
    11041 cgtctgccga aattcggctt cacttctcgt aaagcagcga ttacagccga agttcgtctg
    11101 tctgacctgg ctaaagtaga aggcggtgta gtagacctga acacgctgaa agcggctaac
    11161 attatcggta tccagatcga gttcgcgaaa gtgatcctgg ctggcgaagt aacgactccg
    11221 gtaactgttc gtggcctgcg tgttactaaa ggcgctcgtg ctgctatcga agctgctggc
    11281 ggtaaaatcg aggaataagt agcagatggc taaacaaccg ggattagatt ttcaaagtgc
    11341 caaaggtggc ttaggcgagc tgaaacgcag actgctgttt gttatcggtg cgctgattgt
    11401 gttccgtatt ggctctttta ttccgatccc tggtattgat gccgctgtac ttgccaaact
    11461 gcttgagcaa cagcgaggca ccatcattga gatgtttaac atgttctctg gtggtgctct
    11521 cagccgtgct tctatctttg ctctggggat catgccgtat atttcggcgt cgatcattat
    11581 ccagctgctg acggtggttc acccaacgtt ggcagaaatt aagaaagaag gggagtctgg
    11641 tcgtcgtaag atcagccagt acacccgcta cggtactctg gtgctggcaa tattccagtc
    11701 gatcggtatt gctaccggtc tgccgaatat gcctggtatg caaggcctgg tgattaaccc
    11761 gggctttgca ttctacttca ccgctgttgt aagtctggtc acaggaacca tgttcctgat
    11821 gtggttgggc gaacagatta ctgaacgagg tatcggcaac ggtatttcaa tcattatctt
    11881 cgccggtatt gtcgcgggac tcccgccagc cattgcccat actatcgagc aagcgcgtca
    11941 aggcgacctg cacttcctcg tgttgctgtt ggttgcagta ttagtatttg cagtgacgtt
    12001 ctttgttgta tttgttgagc gtggtcaacg ccgcattgtg gtaaactacg cgaaacgtca
    12061 gcaaggtcgt cgtgtctatg ctgcacagag cacacattta ccgctgaaag tgaatatggc
    12121 gggggtaatc ccggcaatct tcgcttccag tattattctg ttcccggcga ccatcgcgtc
    12181 atggttcggg ggcggtactg gttggaactg gctgacaaca atttcgctgt atttgcagcc
    12241 tgggcaaccg ctttatgtgt tactctatgc gtctgcaatc atcttcttct gtttcttcta
    12301 cacggcgttg gttttcaacc cgcgtgaaac agcagataac ctgaagaagt ccggtgcatt
    12361 tgtaccagga attcgtccgg gagagcaaac ggcgaagtat atcgataaag taatgacccg
    12421 cctgaccctg gttggtgcgc tgtatattac ctttatctgc ctgatcccgg agttcatgcg
    12481 tgatgcaatg aaagtaccgt tctacttcgg tgggacctca ctgcttatcg ttgttgtcgt
    12541 gattatggac tttatggctc aagtgcaaac tctgatgatg tccagtcagt atgagtctgc
    12601 attgaagaag gcgaacctga aaggctacgg ccgataattg gtcgcccgag aagttacgga
    12661 gagtaaaaat gaaagttcgt gcttccgtca agaaattatg ccgtaactgc aaaatcgtta
    12721 agcgtgatgg tgtcatccgt gtgatttgca gtgccgagcc gaagcataaa cagcgccaag
    12781 gctgattttt tcgcatattt ttcttgcaaa gttgggttga gctggctaga ttagccagcc
    12841 aatcttttgt atgtctgtgc gtttccattt gagtatcctg aaaacgggct tttcagcatg
    12901 gaacgtacat attaaatagt aggagtgcat agtggcccgt atagcaggca ttaacattcc
    12961 tgatcataag catgccgtaa tcgcattaac ttcgatttat ggcgtcggca agacccgttc
    13021 taaagccatc ctggctgcag cgggtatcgc tgaagatgtt aagatcagtg agctgtctga
    13081 aggacaaatc gacacgctgc gtgacgaagt tgccaaattt gtcgttgaag gtgatctgcg
    13141 ccgtgaaatc agcatgagca tcaagcgcct gatggatctt ggttgctatc gcggtttgcg
    13201 tcatcgtcgt ggtctcccgg ttcgcggtca gcgtaccaag accaacgcac gtacccgtaa
    13261 gggtccgcgc aaaccgatca agaaataatc ggggtgattg aataatggca aaggcaccaa
    13321 ttcgtgcacg taaacgtgta agaaaacaag tctctgacgg cgtggctcat atccatgctt
    13381 ctttcaacaa caccatcgtg actatcactg atcgtcaggg taacgcgttg ggttgggcaa
    13441 cagccggtgg ttccggtttc cgtggttctc gcaaatccac tccgtttgca gctcaggttg
    13501 cagcagagcg ttgcgctgac gccgtgaaag aatacggcat caagaatctg gaagttatgg
    13561 ttaaaggtcc gggtccaggc cgcgaatcta ctattcgtgc tctgaacgcc gcaggtttcc
    13621 gcatcactaa cattactgat gtgactccga tccctcataa cggttgtcgt ccgccgaaaa
    13681 aacgtcgcgt ataacgcctc gttttccagg tttgttggag aaagaaaatg gcaagatatt
    13741 tgggtcctaa gctcaagctg agccgtcgtg agggcaccga cttattcctt aagtctggcg
    13801 ttcgcgcgat cgataccaag tgtaaaattg aacaagctcc tggccagcac ggtgcgcgta
    13861 aaccgcgtct gtctgactat ggtgtgcagt tgcgtgaaaa gcaaaaagtt cgccgtatct
    13921 atggtgtgct ggagcgtcag ttccgtaact actacaaaga agcagcacgt ctgaaaggca
    13981 acaccggtga aaacctgttg gctctgctgg aaggtcgtct ggacaacgtt gtataccgta
    14041 tgggcttcgg tgccactcgt gcagaagcac gtcagctggt tagccataaa gcaattatgg
    14101 taaacggtcg tgttgttaac atcgcttctt atcaggttag tccgaatgac gttgtaagca
    14161 ttcgtgagaa agcgaagaag cagtctcgcg tgaaagccgc tctggagctg gctgagcagc
    14221 gtgaaaagcc aacctggctg gaagttgatg ctggcaagat ggaaggtacg tttaagcgta
    14281 agccggagcg ttctgatctg tctgcggaca ttaacgaaca cctgatcgtc gagctttact
    14341 ccaagtaaag cttagtacca aagagaggac acaatgcagg gttctgtgac agagtttcta
    14401 aaaccgcgcc tggttgatat cgagcaagtg agttcgacgc acgccaaggt gacccttgag
    14461 cctttagagc gtggctttgg ccatactctg ggtaacgcac tgcgccgtat tctgctctca
    14521 tcgatgccgg gttgcgcggt gaccgaggtt gagattgatg gtgtactaca tgagtacagc
    14581 accaaagaag gcgttcagga agatatcctg gaaatcctgc tcaacctgaa agggctggcg
    14641 gtgagagttc agggcaaaga tgaagttatt cttaccttga ataaatctgg cattggccct
    14701 gtgactgcag ccgatatcac ccacgacggt gatgtcgaaa tcgtcaagcc gcagcacgtg
    14761 atctgccacc tgaccgatga gaacgcgtct attagcatgc gtatcaaagt tcagcgcggt
    14821 cgtggttatg tgccggcttc tacccgaatt cattcggaag aagatgagcg cccaatcggc
    14881 cgtctgctgg tcgacgcatg ctacagccct gtggagcgta ttgcctacaa tgttgaagca
    14941 gcgcgtgtag aacagcgtac cgacctggac aagctggtca tcgaaatgga aaccaacggc
    15001 acaatcgatc ctgaagaggc gattcgtcgt gcggcaacca ttctggctga acaactggaa
    15061 gctttcgttg acttacgtga tgtacgtcag cctgaagtga aagaagagaa accagagttc
    15121 gatccgatcc tgctgcgccc tgttgacgat ctggaattga ctgtccgctc tgctaactgc
    15181 cttaaagcag aagctatcca ctatatcggt gatctggtac agcgtaccga ggttgagctc
    15241 cttaaaacgc ctaaccttgg taaaaaatct cttactgaga ttaaagacgt gctggcttcc
    15301 cgtggactgt ctctgggcat gcgcctggaa aactggccac cggcaagcat cgctgacgag
    15361 taaccggatc acaggttaag gttttactga gaaggataag gtcatgcgcc atcgtaagag
    15421 tggtcgtcaa ctgaaccgca acagcagcca tcgccaggct atgttccgca atatggcagg
    15481 ttcactggtt cgtcatgaaa tcatcaagac gactctgcct aaagcgaaag agctgcgccg
    15541 cgtagttgag ccgctgatta ctcttgccaa gactgatagc gttgctaatc gtcgtctggc
    15601 attcgcccgt actcgtgata acgagatcgt ggcaaaactg tttaacgaac tgggcccgcg
    15661 tttcgcgagc cgtgccggtg gttacactcg tattctgaag tgtggcttcc gtgcaggcga
    15721 caacgcgccg atggcttaca tcgagctggt tgatcgttca gagaaagcag aagctgctgc
    15781 agagtaatct gaagcaacgt aaaaaaaccc gccccggcgg gtttttttat acccgtagta
    15841 tccccactta tctacaatag ctgtactctt tttgttcatc ccctggagta tttatgtggt
    15901 tacttgacca gtgggcagag cgccatatag cagaagcgca agcgaaaggt gagtttgata
    15961 acctggcagg tagcggcgaa ccattgatac tggatgatga ttctcacgtg ccaccggaat
    16021 tacgtgcggg gtatcgcttg ctgaagaatg ccggttgctt accgccagaa cttgagcaac
    16081 ggagagaagc aattcagctt ctggatattc tcaaaggtat ccgtcacgat gatccgcaat
    16141 atcaagaggt tagccgtcga ttgtcattac tggaattgaa gctgcgacaa gctggattga
    16201 gtaccgattt tttacgcggc gattatgctg acaagttgtt ggacaaaatc aacgataact
    16261 agtggagtat gtatgtatcg cattggtgag ctggcaaaaa tggcggaagt aacacccgac
    16321 acgattcgtt attacgaaaa acagcagatg atggagcatg aagtgcgtac tgaaggtggg
    16381 tttcgcctat ataccgaaag cgatctccag cgattgaaat ttatccgcca tgccagacaa
    16441 ctaggtttca gtctggagtc gatccgcgag ttgctgtcga tccgcatcga tcctgaacac
    16501 catacctgtc aggagtcaaa aggcattgtg caggaaagat tgcaggaagt cgaagcacgg
    16561 atagccgagt tgcagagtat gcagcgttcc ttgcaacgcc ttaacgatgc ctgttgtggg
    16621 actgctcata gcagtgttta ttgttcgatt cttgaagctc ttgaacaagg ggcgagtggc
    16681 gttaagagtg gttgttgatt ttttgcactg gcaggattac actcgcgccg ttaaataacc
    16741 aactggagtt tttatgagtc gatatcagca tactaaaggg cagataaagg ataatgcgat
    16801 agaagcatta ctacatgatc ccttattccg acagcgcgta gagaaaaata agaaggggaa
    16861 aggcagttac atgcgaaaag gaaaacatgg caatcggggg aactgggagg ccagtggcaa
    16921 gaaagtaaat cactttttta ccactggtct tctgctttca ggcgcttgtt aagagcggtt
    16981 attctgctct ttcagcaaat cacgaatttc tgtcagtaat acttcttctt tagttggtgc
    17041 aggtgcggct gctggttctt cttttttccg attcagtttg ttgattagct taatcgccat
    17101 aaagatggca aaggccacaa tcagaaaatc aaagacgttt tgaatgaaga caccgtaatg
    17161 catcacaaca gcagggatat ccccctgcgc atcgcgtagc gtgacagcaa actgtttaaa
    17221 atcgatcccg ccaattaata agcccagagg aggcatgatg atatcggcaa ccagtgaaga
    17281 gacaatcttc ccgaatgccg caccgataat gacacccacc gccaaatcca ccacgttccc
    17341 gcgcatcgca aattcgcgaa attctttaat aatgctcatg ttattctccc tatgaagccg
    17401 acaaccataa gtctaacaaa tgttaagcca ttttcctgcc aggaatgaat ttaattaatc
    17461 cttgataatc aaagggaaat aataaggcgt cattagacgc cttattaatt acaagaagaa
    17521 agggcttggc tggaagagtc tttcgacgtc ggtaataaac tttttatctg tgaggaacat
    17581 aattacgtga tcgccttgct caatgcgcag attgtcattg gcaatcatca cgtcgtttcc
    17641 acgtaccacc gctccaataa tcgttcctgg cggtagcttg atttcgtcaa tgactctgcc
    17701 gacaacgcgt gaggtgcttt catcaccgtg agcaacggct tcaatagctt ctgctacgcc
    17761 gcggcgcaat gaggaaacac caacaatatc tgcttttcgc acatggctaa gcaacgcaga
    17821 aatagttgct tgttgtggtg aaatcgcaat atcgataacg ctcccctgaa ccagatccac
    17881 ataagcgcga cgctggatca ataccatcac ctttttcgca cccatacgtt tggcaagcat
    17941 ggcggacatg atattggcct cgtcatcgtt ggtgacggca ataaacagat caacttgatc
    18001 gatatgttct tcggccagta gttcttgatc cgacgcatca ccaaaaaaga cgatcgtatt
    18061 ctgtaacttt tccgccagtt cggcagcgcg ctgctgatta cgttcgatga gtttaacgct
    18121 gtaatctttt tccagacgac gcgccagccc tgcaccgata ttaccgccac caaccagcat
    18181 gatccgctta tacggttttt ccagtcgctg taattcactc atcaccgcgc ggatatgctg
    18241 tgaagcggca ataaagaaca cttcatcacc agcttcaaca atggtcgaac cttgcggacg
    18301 aatggggcga tcgtggcgga aaattgctgc cacacgagta tcgatatgtg gcatatgttc
    18361 gcgcatggtc gaaagtgcat taccaatcag cgggccgcca taataggctt taaccacagc
    18421 caggctgact ttaccctcag cgaagttcac cacctgcaat gcgccgggat actcaatcag
    18481 tcggtaaata ttatcgataa ccaactgctc tggtgcgatc agatgatcaa tcggcacagc
    18541 atctgaatga aatagcttat cggcatcgcg cacgtagtct ggtgagcgga tacgagcgat
    18601 gcgattaggg gtgttgaaaa gtgagtaggc tacctggcag gcaaccatat tggtttcatc
    18661 tgaactggtt acagcaacca gcatatcggc gtcgtcggca cctgcctccc gcaatacgcg
    18721 tggatgagag ccatgcccct gcacgacccg caggtcaaat ttatcctgta aggtccgcag
    18781 acgctcaccg ttggtatcga caacagtaat atcgttgttc tcgccaacca ggttttccgc
    18841 cagtgtgccg ccaacctggc cggcacccag aatgataatt ttcatcagtc gcgacccgtt
    18901 atctcatcac tttttgatta gcttagcgta aaagaagccg tcgccctctt cggcaccagg
    18961 tagattttgt ttacccggtt gctctggtgt tcctgtttcg caaagttcgg catcagcggt
    19021 acgttgcaaa aaggctttaa tctgcaggct attctcttcc ggtaacaccg aacaggtggc
    19081 atagaccaga gttccaccgg tttttaaatg cggccaaatg gcgtcgagaa tttcagactg
    19141 caattgcgcg agttccggga tatcgcgatc gcgacgtaac catttaatat ctggatggcg
    19201 acgaatcaca ccggttgctg aacaaggcgc atctaataaa atgcgatcaa actgttgctc
    19261 gccacaccat tgagaagggt aacggccatc accttgtttc acggtcgcct tcataccaag
    19321 gcgttttaaa ttgtcgtaaa cccgagagag gcgctgttcg tcgatatcaa ccgcaacaac
    19381 ctgcgcttct ggtgccacct caaggatatg cgttgtttta ccgccggggg cggcacaaag
    19441 atccaaaatg tgttcaccgt tttgtggcgc aagccaggtc atgcaacctt gtgctgatgc
    19501 atcctgaacg gtaacccatc cgtcttcaaa accaggtagc gcatgaacag gtgcaggtgt
    19561 ttccagacgt acagcatcag ggtaatccgc atgcgggaaa cctttcattc ctgcttcatc
    19621 cagcaatgca agccagctgt cgcgggaatg atgcgtacga ttaatacgca gccacattgg
    19681 cggacgctgg ttattggctt cgacgatgga ttgccactgc tctggatacg ctttttgcag
    19741 acgcttcagc aaccaggaag gatgcagata acgtgcatca ctggcattaa actcggctaa
    19801 taactcttct tgctgacgct ggaactggcg taatacaccg tttatcaacc ctttaagttg
    19861 cggacgctta attgcgatag cgccttcaac cgtttcagcc agcgcagcat gaggtggaat
    19921 gcgggtataa agcagttgat acaaaccaac cataatcagg taatgcacgg tccgctgttt
    19981 gccggtcatc ggacgggcca ttaacttatt aatcagccag tctaactgcg aaagcgtacg
    20041 cagtacgcca aagcacaact cttgaagaag tgctttgtct ttatcggaaa ctttttgctg
    20101 gagcggtggc agaatgttgc ttaatgattg cccttgctcg acgacttgtt caacggcctg
    20161 ggccgccatg ctacgtaaat tacgttgttt tttcataagt ataaaaacgc ccggcaagac
    20221 cgggcttaga agagtggact atcagaccag acggttgccc ggaacaaacc attcccgacg
    20281 agagttcagg aggtcttgcg cgctcatcgc tttcttaccc gcaggttgta acgagagcag
    20341 gttcaggatg ccatcaccag tcgcaacctg aatgccttgt ttgttggctt caaggatcgt
    20401 tcctggtgca gcgttggttg ccgtatcaat gaccgatgct ttccagactt taaccggctg
    20461 tccttcaatt tccagccagc tcattggcca tggattgaaa gcgcgaatgc agcgttcaag
    20521 ctgtgctgcc gaaagtgacc agtcaatacg cgcttcttct ttactcaact tctcggcgta
    20581 agtgacaaga gtttcgtcct gaacttctgg tttcgccgtg ccgtctgcca gttgtttcaa
    20641 cgtggtgata agcccttgtg ggccaagctc tgccagcttg tcgtacagcg taccactggt
    20701 atcttctgca gtaatcgggc aggagagctt atagagcata tcaccggtgt ctaaaccgac
    20761 atccatttgc ataatggtca caccagtttc tgcatcaccc gcccatagtg agcgttggat
    20821 tggtgcagca ccgcgccagc gtggcagcag tgaaccatga acgttgatac agccaagacg
    20881 cggcatctcc agcactgctt tcggcagaat taaaccatag gcgacgacga ccataacatc
    20941 agcctgcagt tcggcgacca gttgctggtt ttcttgtgga cgcagggaaa caggttgaaa
    21001 aacgggcaga cctttttcct cagccagaac tttaatcggg ctgggcatca gttttttacc
    21061 gcgtcctgcc ggtcggtctg gctgggtgaa cacgccaacg acgttatgac cagaagacaa
    21121 cagcgcgtcg agatgacgcg ctgcaaagtc aggtgtaccc gcaaaaataa tacgtagtga
    21181 ttctgacacg ttagttctta tccttaagcc cggtctttca gacgatccag tttttcaact
    21241 ttctgacgaa tacgttgttg tttcagcggt gacagataat ccataaacag tttgccgacc
    21301 aagtgatcca tctcatgctg aatacagatg gctaacagac cgtctgcttc cagttcaaat
    21361 ggtttaccgt cgcggtcaag ggcgcgaatt ttaactttct ctgcgcgcgg cactaaagca
    21421 cgttgttcag ggatcgacag gcaaccttct tcaatgcctg tttcgccgct tttttctaaa
    21481 agctccggat tgattaacac tagccgttcg tcacggtttt ccgaaacatc aataacaatg
    21541 atacgttgat ggatatcaac ctgggttgcc gccaggccaa taccttcttc tgcgtacatc
    21601 gtctcgaaca tatcatcgac gatacgctga atttctgcat tcacttcttc taccggttta
    21661 gcaactttgc gaagccgctc gtccggaata tgtaacactt gcaaaactga cataaatctc
    21721 cagagatgtg ttcaggagtt agaaagatta tttcttctat tctagacaaa tccccctctg
    21781 attgacagca tcactgacca atcgcaaaga ttgctaaggc tgcttatggc agggagataa
    21841 ggatggtcga tacagaaatt tggctgcgtt taatgagtat cagcagcttg tacggcgatg
    21901 atatggtccg tatcgctcac tgggtggcaa aacagtcgca tattgatgcg gttgtattgc
    21961 agcaaacagg gcttacattg cggcaggcac aacgctttct ttcatttcca cgaaagagta
    22021 tcgaaagctc actttgttgg ttggagcaac ccaaccatca tttaatccct gcggacagcg
    22081 aattttatcc tcctcaactt ctggcgacga cagattaccc cggcgcactg tttgttgaag
    22141 gagaactgca cgcgctgcat tcatttcagc ttgccgtagt ggggagtcgg gcgcattcat
    22201 ggtatggcga gcgatgggga cgattatttt gcgaaactct ggcgacgcgt ggagtgacaa
    22261 ttacgagtgg actggcgcgt ggaatcgatg gtgtggcgca taaagcggcc ttacaggtaa
    22321 atggcgtcag tattgctgta ttagggaatg gacttaatac cattcatccg cgtcgccatg
    22381 cccgactggc taccagtttg cttgaacatg gtggggctct tgtctcggaa tttcccctcg
    22441 atgttccacc ccttgcttac aatttcccac gaagaaatcg cattatcagt ggtttaagta
    22501 aaggcgtact ggtggtggaa gctgctttgc gcagtggttc gctggtgaca gcacgttgtg
    22561 cgcttgagca ggggcgtgaa gtttttgcct tgcctggtcc attagggaat ccgggaagcg
    22621 aagggcctca ctggttaata aaacaaggtg cgattcttgt gacggaaccg gaagaaattc
    22681 tggaaaactt gcaatttgga ttgcactggt tgccagacgc ccctgaaaat tcattttatt
    22741 caccagatca ggaagacgtg gcattgccat ttcctgagct cctggctaac gtaggagatg
    22801 aggtaacacc tgttgacgtc gtcgctgaac gtgccggcca acctgtgcca gaggtagtaa
    22861 ctcaattact cgaactggag ttagcaggat ggatcgcagc tgtacccggc ggctatgtcc
    22921 gattgaggag ggcatgccat gttcgacgta ctaatgtatt tgtttgaaac ctatattcac
    22981 acagaagctg agttgcgtgt ggatcaagac aaacttgaac aggatcttac cgacgcagga
    23041 tttgatcgag aagatatcta caatgccctg ctttggctgg aaaagcttgc ggattatcag
    23101 gaaggactgg cagaaccgat gcaactggcc tctgatccac tctccatgcg tatttatacc
    23161 cctgaggagt gtgaaagact ggatgccagc tgtcgtggtt ttctgctttt ccttgagcag
    23221 attcaggtgc tcaaccttga aactcgtgaa atggtgatag agcgagtgct cgcgctggat
    23281 aacgcagagt tcgaactgga tgatctgaaa tgggtaatcc tgatggtgtt gttcaatatt
    23341 ccgggctgcg aaaatgcgta ccagcaaatg gaagaattac tctttgaagt gaatgaaggt
    23401 atgctgcatt aatcatttct ttaattcagc ataagttgtt atggcgaaat cagcactgtt
    23461 cacggtgcgt aataatgagt cctgcccaaa gtgcggggct gaactggtta ttcgatccgg
    23521 gaaacacggt ccgtttcttg gatgctcaca gtatccggcg tgtgactacg tccgtcctct
    23581 gaaatcttca gcggatggac atattgtcaa agttctggag gggcaggttt gccctgcatg
    23641 tggcgcaaat ctggtattac gccagggacg ctttggtatg tttattggtt gcagtaacta
    23701 ccctgaatgc gaacataccg aacttatcga taaaccggac gaaacagcaa ttacatgccc
    23761 ccaatgccgg acgggccatc tggtccagcg ccgctcccgt tatggcaaaa catttcactc
    23821 ctgtgatcgc tacccggagt gtcaatttgc cattaacttc aaacccatag ctggagaatg
    23881 ccctgagtgt cattatccac tactcatcga aaagaaaacc gcgcagggtg taaaacactt
    23941 ttgtgccagt aaacaatgtg gaaagccggt ttcggcggaa taataacgtg aataataacc
    24001 tgcaaggaga cgctatcgca gctgcgatag atgtcctcaa tgaagaacgt gtcatcgcct
    24061 atccaacgga agccgttttc ggtgttgggt gcgatcctga tagcgaaaca gcagtgatga
    24121 gactgttgga gttaaaacag cgtccggttg ataaggggct gattttaatc gcggcaaatt
    24181 acgagcagct taaaccctat attgatgaca ccatgctgac tgatgcgcag cgtgaaacca
    24241 ttttttcccg ctggccaggt cctgtcacct ttgtctttcc cgcgcctgcg acaacaccgc
    24301 gctggttgac gggccgcttt gattcgcttg ctgtacgagt caccgaccat ccgttggtgg
    24361 ttgctttgtg ccaggcttat ggtaaaccgc tggtttctac cagtgccaac ttgagtggat
    24421 tgccaccttg tcgaacagta gacgaagttc gcgcacaatt tggcgcggcg ttcccggttg
    24481 tgcctggtga aacggggggg cgtttaaatc cttcagaaat ccgcgatgcc ctgacgggtg
    24541 aactgtttcg acaggggtaa cataatggaa acctatgctg tttttggtaa tccgatagcc
    24601 cacagcaaat cgccattcat tcatcagcaa tttgctcagc aactgaatat tgaacatccc
    24661 tatgggcgcg tgttggcacc catcaatgat ttcatcaaca cactgaacgc tttctttcgt
    24721 gctggtggta aaggtgcgaa tgtgacggtg ccttttaaag aagaggcttt tgccagagcg
    24781 gatgagctta ctgaacgggc agcgttggct ggtgcagtta ataccctcaa gcggttagaa
    24841 gatggacgcc tgctgggtga caataccgat ggtgtaggct tgttaagcga tctggaacgt
    24901 ctgtctttta tccgccctgg tttacgtatt ctgcttatcg gcgctggtgg agcatctcgc
    24961 ggcgtactac tgccacttct ttccctggac tgtgcggtga caatcactaa tcggacggta
    25021 tcccgcgcgg aagagttggc taaattgttt gcgcacactg gtagtattca ggcgttgagt
    25081 atggacgaac tggaaggtca tgagtttgat ctcattatta atgcaacatc cagtggcatc
    25141 agtggtgata ttccggcgat cccgtcatcg ctcattcatc caggcattta ttgctatgac
    25201 atgttctatc agaaaggaaa aactcctttt ctggcatggt gtgagcagcg aggctcaaag
    25261 cgtaatgctg atggtttagg aatgctggtg gcacaggcgg ctcatgcctt tcttctctgg
    25321 cacggtgttc tgcctgacgt agaaccagtt ataaagcaat tgcaggagga attgtccacg
    25381 tgaatcaggc catccagttt ccggacaggg aagagtggga cgagaataaa aaatgtgtat
    25441 gttttcccgc tctcgtgaat ggtatgcaac tgacatgcgc gatctctggc gagagtctgg
    25501 cgtatcgctt tactggagat acgccagaac agtggttagc gagttttcgt cagcatcgct
    25561 gggacctgga agaagaagcg gaaaacttaa ttcaggaaca aagtgaagat gatcaaggct
    25621 gggtctggtt accctgatcc agatattcgt ccttccattt cacgtaatta ttcgcggaat
    25681 agcgtaaccc agccttctct tcatcactta acgggcggat ctgtttgacg gggctaccga
    25741 gatacagata tccgctctcc agccgtttat tttgtgggac cagactaccc gcaccaatca
    25801 tcacatcatc ttctactatt gcgccatcaa gtaaaattga gcccatccca accaaaactc
    25861 gattgccaat ggtgcagccg tggagcatca ccttgtgacc aacagtgaca tcttcgccaa
    25921 tggttaatgg gttgccatct gggttgtacg aggatttatg agtgacatgc aacatactgc
    25981 catcctggat attggtgcgt gctccgatct gtacataatg tacatctcca cgaatcacaa
    26041 cgagcggcca gatccccaca tcatcagcca gacgaacgtc accaatcacg acactgctat
    26101 cgtcgatcat tacgcgctga ccgatttgtg gaaaaagatc gcggtatggg cgtaaaacat
    26161 cagacatact tacctcagca ataaatgatt tactaatgac tttgggggca ttattggcct
    26221 tgtgcaagtc ttttagtatg caaaaaagca ccgttttgtg tgcgattgca gcaaaaaggg
    26281 tgaaaaaaca acaaacagaa aaaaagatca aaaaaatact tgtgcaaaaa attgggatcc
    26341 ctataatgcg cctccgttga gacgacaacg tgaaacactt cacaggatgg tcggaacaac
    26401 gaagaga
//
LOCUS       DAJOSY010000061        26406 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJOSY010000061 DAJOSY010000000
VERSION     DAJOSY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.061"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            29..550
                     /gene="fecI"
                     /locus_tag="OHN27_002647"
     CDS             29..550
                     /gene="fecI"
                     /locus_tag="OHN27_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCQ2287060.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            547..1500
                     /gene="fecR"
                     /locus_tag="OHN27_002648"
     CDS             547..1500
                     /gene="fecR"
                     /locus_tag="OHN27_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCQ2287061.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1587..3911
                     /gene="fecA"
                     /locus_tag="OHN27_002649"
     CDS             1587..3911
                     /gene="fecA"
                     /locus_tag="OHN27_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCQ2287062.1"
                     /translation="MTPLRVFRKTTPLVNTIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            3956..4858
                     /gene="fecB"
                     /locus_tag="OHN27_002650"
     CDS             3956..4858
                     /gene="fecB"
                     /locus_tag="OHN27_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCQ2287063.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            4855..5853
                     /gene="fecC"
                     /locus_tag="OHN27_002651"
     CDS             4855..5853
                     /gene="fecC"
                     /locus_tag="OHN27_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCQ2287064.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            5850..6806
                     /gene="fecD"
                     /locus_tag="OHN27_002652"
     CDS             5850..6806
                     /gene="fecD"
                     /locus_tag="OHN27_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCQ2287065.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            6807..7574
                     /gene="fecE"
                     /locus_tag="OHN27_002653"
     CDS             6807..7574
                     /gene="fecE"
                     /locus_tag="OHN27_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCQ2287066.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8132..8389)
                     /locus_tag="OHN27_002654"
     CDS             complement(8132..8389)
                     /locus_tag="OHN27_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287067.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            8536..9603
                     /locus_tag="OHN27_002655"
                     /pseudo
     CDS             8536..9603
                     /locus_tag="OHN27_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
     gene            complement(9616..9873)
                     /locus_tag="OHN27_002656"
                     /pseudo
     CDS             complement(9616..9873)
                     /locus_tag="OHN27_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9974..10547
                     /locus_tag="OHN27_002657"
                     /pseudo
     CDS             9974..10547
                     /locus_tag="OHN27_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10596..11420)
                     /locus_tag="OHN27_002658"
     CDS             complement(10596..11420)
                     /locus_tag="OHN27_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287068.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSDKTLLTAIRDYIYGEAWYLVYDLIE
                     FIIERTNSYINLSKHLNSIFKKHGVGYTIINGCITPISNDNEIESVQNAVDNGTDSSR
                     SHFERALQLMTDREQPDYRNSIKESISAIESLCRKITGNDKGTLGACLKAIEEKGYIH
                     SAMKGAFSQLYGYTSDQGGIRHALTEEDVNPTLAEAKFMLVTCSAFSNYLLSKISD"
     gene            complement(11954..12038)
                     /locus_tag="OHN27_002659"
     tRNA            complement(11954..12038)
                     /locus_tag="OHN27_002659"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12002..12004),aa:Leu,seq:caa)
     gene            12234..13253
                     /gene="ahr"
                     /locus_tag="OHN27_002660"
     CDS             12234..13253
                     /gene="ahr"
                     /locus_tag="OHN27_002660"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCQ2287069.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            13383..14885
                     /locus_tag="OHN27_002661"
     CDS             13383..14885
                     /locus_tag="OHN27_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCQ2287070.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRTFTPKDQKAV
                     KAAAQTMRVNPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASAEQQNNPAVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(15046..16128)
                     /gene="lptG"
                     /locus_tag="OHN27_002662"
     CDS             complement(15046..16128)
                     /gene="lptG"
                     /locus_tag="OHN27_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCQ2287071.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(16128..17228)
                     /gene="lptF"
                     /locus_tag="OHN27_002663"
     CDS             complement(16128..17228)
                     /gene="lptF"
                     /locus_tag="OHN27_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCQ2287072.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMCTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            17495..19006
                     /gene="pepA"
                     /locus_tag="OHN27_002664"
     CDS             17495..19006
                     /gene="pepA"
                     /locus_tag="OHN27_002664"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCQ2287073.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            19360..19803
                     /gene="holC"
                     /locus_tag="OHN27_002665"
     CDS             19360..19803
                     /gene="holC"
                     /locus_tag="OHN27_002665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCQ2287074.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            19803..22658
                     /gene="valS"
                     /locus_tag="OHN27_002666"
     CDS             19803..22658
                     /gene="valS"
                     /locus_tag="OHN27_002666"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCQ2287075.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(22712..23908)
                     /locus_tag="OHN27_002667"
     CDS             complement(22712..23908)
                     /locus_tag="OHN27_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCQ2287076.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVDCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSFVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            24101..24604
                     /locus_tag="OHN27_002668"
     CDS             24101..24604
                     /locus_tag="OHN27_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2287077.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     RLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(24650..25066)
                     /gene="rraB"
                     /locus_tag="OHN27_002669"
     CDS             complement(24650..25066)
                     /gene="rraB"
                     /locus_tag="OHN27_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCQ2287078.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            25228..26232
                     /gene="argF"
                     /locus_tag="OHN27_002670"
     CDS             25228..26232
                     /gene="argF"
                     /locus_tag="OHN27_002670"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCQ2287079.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNNKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT         6599 a         6809 c         6912 g         6086 t
ORIGIN      
        1 gtgataatta acttttgatg cactccgcat gtctgaccgc gccactacca cagcttcctt
       61 aacgttcgag tcgctttatg gcacacatca cggctggttg aaaagctggc tgacgcgcaa
      121 actccagtct gcttttgatg cagatgacat tgcccaggac acttttttgc gggtaatggt
      181 cagcgaaacg ctctcgacga tccgcgatcc tcgctccttc ctctgcacta tcgccaaacg
      241 cgtgatggtg gacctgtttc gccgaaacgc gctggaaaaa gcgtatctgg agatgctggc
      301 gcttatgccg gaggggggag cgccttcacc tgaggaacgc gaaagccaac tcgagaccct
      361 acaactcctc gacagcatgc tggacgggct aaacggcaaa acacgtgaag cgtttctgct
      421 ttcgcaactg gatggtctga catacagcga gattgcgcac aaactcggtg tttccatcag
      481 ctccgtgaaa aaatacgtgg cgaaagccgt cgagcactgc ctgctgttcc gtctggagta
      541 tgggttatga atcctttgtt aaccgattcc cgccgtcagg cgctgcgttc agcttcccac
      601 tggtatgccg tgctaagcgg cgagcgcgtc agcccacaac aggaagcgcg ctggcaacag
      661 tggtatgaac aggatcagga taaccagtgg gcctggcagc aggttgaaaa cctgcgcaac
      721 cagcttggcg gtgtgcctgg cgacgttgcc agcagggcgt tgcacgatac ccgcctcacc
      781 cgccgtcacg tgatgaaagg attactgctg ttgctcggcg ctggcggagg ctggcagctc
      841 tggcagtcgg aaaccggcga aggtctgcgg gcagattacc gcaccgccaa aggcacagtc
      901 agccgtcagc aactggaaga tggctccctg ctcacgctga atacccaaag cgcggcggat
      961 gtgcgttttg atgcgcatca gcgcaccgtc cggctctggt acggtgaaat cgccattact
     1021 accgcgaaag atgccctgca acgccccttc cgtgtcctga cccgtcaggg ccagctcact
     1081 gctttaggga cagaatttac cgtccgccag caggataatt tcacgcagct tgacgtgcag
     1141 cagcacgctg tggaagtgct tctcgccagt gcccccgcgc aaaaacgcat cgtgaacgct
     1201 ggtgaaagcc tgcagttcag cgcctctgag tttggcgcag tgaaaccgct ggatgacgag
     1261 agtacaagct ggacgaagga catcctgagc ttcagcgata aaccgctggg tgaggtgata
     1321 gccacgctaa cccgttaccg caacggcgtg ctgcgctgcg atcccgccgt tgccgggctg
     1381 cgcctgagcg ggacgttccc gctgaaaaat accgatgcga tcctgaacgt tatcgcgcaa
     1441 acgcttcccg ttaaaattca gtctattacg cggtactgga taaacatttc accactgtaa
     1501 ggaaaataat tcttatttcg attgtccttt ttacccttct cgttcgactc atagctgaac
     1561 acaacaaaaa tgatgatggg gaaggtatga cgccgttacg cgtttttcgt aaaacaacac
     1621 ctttggttaa caccattcgc ctgagcctgc tgccgctggc cggtctctcg ttttccgctt
     1681 ttgctgcaca ggttaatatc gcaccgggat cgctcgataa agcgctcaat cagtatgccg
     1741 cacacagcgg atttaccctc tcggttgatg ccagcctgac gcgcggcaag cagagcaacg
     1801 gcctgcacgg cgattacgac gtcgagagcg gcctgcaaca actgctggac ggcagcggac
     1861 tgcaggtaaa accgctggga aataacagct ggacgctgga gcccgcgccc gcaccaaaag
     1921 aagatgccct gaccgtggtc ggcgactggc tgggtgatgc gcgtgaaaac gacgtatttg
     1981 aacatgctgg cgcgcgtgac gtgatccgcc gtgaggattt cgccaaaacc ggcgcaacca
     2041 ccatgcgtga ggtacttaac cgcatccctg gcgtcagcgc gccggaaaac aacggcaccg
     2101 gcagccacga cctggcgatg aactttggca tccggggcct gagcccgcgc ctcgccagcc
     2161 gctcgaccgt cctgatggac ggcatccccg tccccttcgc cccttacggt cagccgcagc
     2221 tttcactggc tcccgtttcg ctcggcaaca tggatgccat tgacgtggta cgcggtggtg
     2281 gtgcggtgcg ttacggaccg cagagcgtgg gcggcgtggt gaactttgtt acccgtgcca
     2341 ttccgcagga ctttggtatc gaggcgggcg tggaaggtca gctcagccca acctcttcac
     2401 aaaacaaccc gaaagagacg cacaacctga tggtgggcgg cacagcggac aacggttttg
     2461 gcaccgcgct gctctactcc ggcacgcgcg gcagtgactg gcgcgagcac agcgccaccc
     2521 gcatcgacga cctgatgctg aaaagcaaat atgcgccgga tgaggtgcac accttcaaca
     2581 gcctgctgca atattacgat ggtgaagccg acatgcccgg tggcctgtct cgcgcggatt
     2641 acgacgccga tcgctggcaa tccacccgcc cgtatgaccg cttctggggt cgtcgcaagc
     2701 tggcgagcct gggctaccag ttccagccag acagccagca taaattcaac attcaggggt
     2761 tctacaccca aaccctgcgc agcggctacc tggagcaagg caaacgcatc accctctcgc
     2821 cgcgtaacta ctgggtgcgc ggtattgagc cacgctacag ccagatcttt atgatcggcc
     2881 cttccgcgca cgaagtgggc gtgggctatc gctatttgaa tgaatcaacg catgaaatgc
     2941 gttactacac cgccaccagc agcgggcagt tgccgtccgg ctcaagccct tacgaccgcg
     3001 atacgcgttc cggcaccgag gcgcacgcct ggtatctgga tgacaaaatc gacatcggca
     3061 actggaccat cacgccgggt atgcgtttcg aacatatcga gtcataccag aacaacgcca
     3121 tcacaggcac gcacgaagaa gtgagctata acgcaccgct tccggcgttg aacgtgctct
     3181 atcacctgac tgacagctgg aatctttatg caaacactga aggctcgttc ggcaccgtac
     3241 agtacagcca gattggcaag gctgtgcaaa gcggcaatgt ggaaccggaa aaagcgcgaa
     3301 cctgggaact cggtacccgc tacgacgacg gcgcgctgac ggcggaaatg gggctgttcc
     3361 tgattaactt taacaatcag tacgactcca accagaccaa cgacaccgtc actgcacgtg
     3421 gcaaaacgcg ccataccggg ctggaaacgc aggcacgtta cgatctgggt acgctaacgc
     3481 caacgcttga taacgtttcc atctacgcca gctatgcgta tgtgaacgcg gaaatccgcg
     3541 agaaaggcga cacctacggc aatctggtac cattctcccc gaaacataaa ggcacgctgg
     3601 gcgtggacta caagccagga aactggacgt tcaatctgaa cagcgatttc cagtccagcc
     3661 agtttgcgga taacgccaat acggtgaaag agagcgccga cggcagtacc ggccgcattc
     3721 ccggcttcat gctctggggc gcacgcgtgg cgtatgactt tggcccgcag atggcagatc
     3781 tgaacctggc attcggtgtg aaaaacatct tcgaccagga ctacttcatc cgctcttatg
     3841 acgacaacaa caaaggcatc tatgcaggcc agccgcgcac gctgtatatg caggggtcgt
     3901 tgaagttctg atgtgttaac gcccggcttg ccgggctttt agctggaatg tgattatgtt
     3961 ggcatttatc cgttttcttt ttgcaggcct gctgctggtg atcagccacg cctttgccgc
     4021 catggttcag gacgaacacg gcacgtttac actcgaaaaa acgccacaac ggattgtggt
     4081 gctggaactc tcgttcgccg atgcgctggc cgccgtggac gtcagcccga tcggtattgc
     4141 cgacgataac gatgcaaaac gcatcctgcc cgaagtgcgt gcgcacctga aaccgtggca
     4201 gtccgtcgga acgcgcgcgc agccgagcct ggaagccatt gccgctctga aaccagacct
     4261 gatcattgcc gacagcagtc gccatgcggg ggtttacatc gccttgcagc aaatcgcgcc
     4321 ggtactgctg cttaagtccc gcaacgaaac ctacgctgaa aatttgcaat ctgcggctat
     4381 catcggcgaa atggtgggta aaaagcgaga gatgcaggca cgtctggaac aacataaaga
     4441 gaggatggcg cagtgggcca gccagcttcc caaagggaca cgcgtggcct ttggcacatc
     4501 acgcgaacag caattcaacc tgcatactca ggagacctgg accggcagcg tgctggcctc
     4561 tctggggctg aacgttcccg ctgcgatggc gggtgcgtcc atgccgtcca tcggcctgga
     4621 gcaactgctg gcggtcaatc ctgcctggct gctggttgcc cactatcgcg aagagagcat
     4681 tgttaaacgc tggcaacaag atccgctctg gcagatgtta accgccgcgc agaagcagca
     4741 ggttgcttcg gtcgacagta acacctgggc gcggatgcgc ggtatttttg ctgcagagcg
     4801 tattgccgct gacacggtaa aaatcttcca ccatcagccg cttaccgttg tgaaatgacc
     4861 gcgataaaac acccggtgct gctgtggggg cttcccgttg cagcacttat tattattttc
     4921 tggctgagtc tgttttgcta ctcggccatt cctgtttccg gagcagatgc aacccgcgcc
     4981 ctgctgcctg gacacacgcc aacgctacca gaagcgctgg tgcaaaacct tcgtttgcca
     5041 cgaagcctgg tcgccgttct gatcggcgca agcctggcgc tcgcgggcac gctgctgcaa
     5101 accctgaccc acaacccaat ggcctctcct tcactgctcg gcattaacag cggcgcggca
     5161 ctggctatgg cgcttaccag cgcgctgagt ccgacgccga ttgcaggcta ttctctgtcg
     5221 ttcatcgcgg catgcggggg cggcgtgagc tggctgctgg tcatgaccgc aggaggcgga
     5281 tttcgtcata cccatgacag aaacaaactg atcctcgcgg gtatcgcgct gtcggccttt
     5341 tgtatgggcc ttacccgcat caccctgctg ctggccgaag atcatgctta cggcatcttt
     5401 tactggctgg caggcggagt gtcccacgcc cgctggcagg atgtctggca gctcttgccg
     5461 gtggtggtca ctgcagtccc tgtcgtgttg ctgctggcga atcaactgaa cctgctcaac
     5521 ctcagcgaca gcaccgccca tacgctggga gtgaacctga cgaggctacg tttggtcatc
     5581 aatatgttag tgctgcttct ggttggtgcg tgcgtcagcg tggcaggtcc ggtggcgttt
     5641 atcggtctgc tggtgccaca tctggcgcgc ttctgggcag gcttcgatca gcgcaacgta
     5701 ctgccagtga gcatgctgct gggggccacg ctgatgctgc tggcagatgt actcgcacgc
     5761 gcgctggcct tccccggaga tctgcccgca ggcgcagtgc tggcgctgat tggcagccct
     5821 tgctttgtct ggcttgtgag gaggcgagga tgaaaattgc gctggttatt ttcatcaccc
     5881 ttgccctggc gggctgtgcg ctgttatcac tccatatggg agtgatcccc gtgccgtggc
     5941 gcgcgctgct gaccgactgg caggccggac gcgagcatta ttatgtattg atggagtacc
     6001 gactgccgcg cttgctgctg gcactgtttg tcggtgcagc cctcgccgtg gcgggcgtgc
     6061 tgatacaggg gattgtgcgc aaccctctgg catcaccgga tattctcggc gttaaccatg
     6121 ccgccagcct ggcctctgtg ggggctctac ttcttatgcc gtcactgccc gtgatggtgc
     6181 tgccgctgct ggcctttgcg ggcggcatgg cggggttgat attactgaag atgctggcaa
     6241 agacccacca gccgatgaag ctggcgctca ccggcgtggc gctttctgca tgctgggcca
     6301 gcctgacgga ttatctgatg ctctcgcgcc cacaggatgt gaacaacgcc ctgctgtggc
     6361 tgaccggcag cttatggggc cgtgactgga gctttgtgaa gattgccatc ccgctgatga
     6421 ttttatttct gccgctgagc ctgagttttt gccgcgatct cgacctcctt gcactcggcg
     6481 atgcgcgcgc caccacgctc ggtgtgtcgg tgccccatac ccgattctgg gctttgttac
     6541 tagctgtcgc catgacatct accggcgtgg ccgcctgcgg cccgattagc tttattggtc
     6601 tcgtggtgcc gcatatgatg cgtagcatca ccggtggacg tcaccgcaga ctgctgcctg
     6661 tttcagccct gacaggtgcg ttgctgttgg tggttgccga tctgctggcg agaattattc
     6721 atcccccact ggagctcccg gttggcgtgc tgaccgccat tatcggtgcg ccgtggtttg
     6781 tctggttgct tgtgagaatg cgataaatga ctttacgaac tgaaaatctg acggtcagtt
     6841 acgggacaga caaggtactt aacgacgttt cactctcact gccaacgggg aagatcaccg
     6901 ccctgatcgg tcctaacggt tgcgggaaat cgacgctgtt aaactgtttt tcgcggcttt
     6961 taatgccgca gtctggcacc gtatttctcg gcgataatcc cataaatatg ctctcatcgc
     7021 gccagttggc ccgcaggctt tcgctgctgc ctcagcacca tttaacgcca gaggggatca
     7081 cagtccagga gctggtttcg tatggtcgta atccctggct gtcactctgg gggcgtctct
     7141 ccgctgaaga caatgcacga gttaatgtcg ccatgaacca gacccggatc aatcatcttg
     7201 ccgttcgtcg gttaaccgag ctttccggcg gtcagcgcca gcgcgcattt ctggcgatgg
     7261 tcctggccca gaatacgccc gttgtattac ttgatgagcc aaccacctat cttgatatca
     7321 atcaccaggt ggacctgatg cggttgatgg gcgaactccg gactcagggg aaaacggtgg
     7381 tcgctgtgct gcacgacctt aatcaggcta gccggtactg cgatcaactg gtggtaatgg
     7441 caaacggaca tgttatggcg caaggcacac cagaagaggt gatgacccca ggattgctga
     7501 gaacagtatt cagcgtggaa gcggaaatac accccgagcc ggtatctggc aggccgatgt
     7561 gcctaatgag gtagattgca caggccgtaa gaaccaaacc acgactgaat gaaactggac
     7621 tggcgccagc aagcctgttc agactggggc tgaacttttc cggactctga aagattacca
     7681 atactcatcg tccatccgct tgctttaggc tgacaggttc ataatcaacg caaaccagag
     7741 ctgtacaggc ttgggcgcgg ctttcaaacc agtcgtgatc acggcaatca attttgaact
     7801 ctgcttaacg gacatttctg tataaccctt acggcaacga aaaacgcgaa gttaaaattt
     7861 tagaaaccca aaaacgtgac atgactaagt ttagatttca gggggggaga tcaaaaaatt
     7921 tcgctctgtg ccagagcgga cattcacgga gctggttcat taccaatgag gttgggcttt
     7981 tgaggataaa tcaatgatca gacgccaacg taaatcaaaa gcaaccctgg aacggaattg
     8041 ctaatccagt ttctgaccat cgatttttct aaaaagtgtg cgtttgctac tacttaggta
     8101 ggtgcagctt tcttaatcac cggcagccac gttatacagg ccagttgatg gatcgattgt
     8161 tatcaatgat atctttatga gtcggtgtct cacccagctt accgaagctg gcataaagtg
     8221 aaggcagacg ggcccgtcct tctccctttt tcgccagagg gaaagcgcga agcatggtgg
     8281 cagcctccca gtcacaacaa taggatggtg tgcacggctg ctgacgccat gattcagcga
     8341 tagagccgga aaatacgggg tcaaagccgg tatcattaac cagagtcatc gtgacctgta
     8401 ctgcggctgg atgatctcct gctactgcaa ctgctaggcg accgcggctc ccttcaggta
     8461 gtctgttgtg gtcaactaaa actggcctcc gcgttagagt ttttccagta tcggttttct
     8521 gattcgtttg gtggtatcct gggttcaaaa cccggaacga tcttcactat gttaagggat
     8581 actggcggca taaaacccca tgagcataag cgggctgtag ctcacctgac actgtctgag
     8641 cgcgaggaga tacgagctgg tttgtcagcc aaaatgagca ttcgtgcgat agctactgcg
     8701 ctgaatcgca gtccttcgac gatctcacgt gaagttcagc gtaatcgggg cagacgctat
     8761 tacaaagctg ttgatgctaa taaccgagcc aacagaatgg cgaaaaggcc aaaaccgtgc
     8821 ttactggatc aaaatttacc attgcgaaag cttgttctgg aaaagctgga gatgaaatgg
     8881 tctccagagc aaatatcagg atggttaagg cgaacaaaac cacgtcaaaa aacgctgcga
     8941 atatcacctg agacaattta taaaacgctg tactttcgta gccgtgaagc gctacaccac
     9001 ctgaatatac agcatctgcg acggtcgcat agccttcgcc atggcaggcg tcatacccgc
     9061 aaaggcgaaa gaggtacgat taacatagtg aacggaacac caattcacga acgttcccga
     9121 aatatcgata acagacgctc tctggggcat tgggagggcg atttagtctc aggtacaaaa
     9181 aactctcata tagccacact tgtagaccga aaatcacgtt atacgatcat ccttagactc
     9241 aggggcaaag attctgtctc agtaaatcag gctcttaccg acaaattcct gagtttaccg
     9301 tcagaactca gaaaatcact gacatgggac agaggaatgg aactggccag acatctagaa
     9361 tttactgtca gcaccggcgt taaagtttac ttctgcgatc ctcagagtcc ttggcagcgg
     9421 ggaacaaatg agaacacaaa tgggctaatt cggcagtact ttcctaaaaa gacatgtctt
     9481 gcccaatata ctcaacatga actagatctg gttgctgctc agctaaacaa cagaccgaga
     9541 aagacactga agttcaaaac accgaaagag ataattgaaa ggggtgttgc attgacagat
     9601 tgaatctaca gtagcctttt ttaatatttc attctccatt tcaatgcgtt gtagcttttt
     9661 cctcagctta cgtatttcga tttgttctgg tgttatcgga gaggcttttg gtgttttgcc
     9721 ctgacgctca tcacgcagtt gtttgaccca tcttgtcatt gtggaaaggc caacatccat
     9781 agctttggcg gcatctgcca ccgtgtatgt ctggtcaaca accagttgag cggattcgcg
     9841 tttaaactct gcgctaaaat ttcttttttt cattggagca cctgtgttgt tctgaggtga
     9901 gcatatcacc tctgttcagg tggccaaatt cagtaaacca cttcaccact tcagagcaga
     9961 cgagacaaaa agaatggttg acgcatcagc tcaaccccat atagttgtaa cacttgagcc
    10021 aaacccttgg gccgcttttt actttgatat taacattgct aatacaggga acgcacctgc
    10081 ctataatgtt gaggttgtgt ttgatcctcc actagtaaat gcggagcata gagaaaaaag
    10141 tgagattccg tttagtaagg taagcgtgtt aaaaaaatgg gcaatcactt accagcaatc
    10201 tctgtaagta tgaacaaatc aaagatcaaa tttataatat taatataagc tgggcaagca
    10261 aacctaaatc aaacgataga gaaacaaatg aatatgtgta tgacatggcg acatttgaag
    10321 gaataagtta tctaggagcg agaagcccat tgacgcaaat tgcagaacaa attaaaggta
    10381 taagagagga ttggaaacct attgcacaag gagctaaaaa agtaaaagca gacgtatata
    10441 cttcaagcga tagaaacgaa gaacgcacgt atctgcaaga gcaacacgat ttggcaataa
    10501 aaaggagaga tgagaaaaga gaaaaaagat tagagtctgg tgaataattt taaagggagt
    10561 gggtaactaa cccactcgta actataaacc tgtaattaat cacttatttt tgacaacaga
    10621 taattactga acgcactgca agtgactaac ataaatttag cttcagctaa ggtaggattt
    10681 acatcttctt ctgttagcgc atgacgtatt ccaccctgat cacttgtata accatagagc
    10741 tgactaaaag cgcctttcat tgcagagtgt atatatcctt tttcctctat agctttaaga
    10801 caagccccca aggttccttt atcattgccc gtgattttcc tgcataaaga ttcaattgca
    10861 gagatagact ctttaatcga gtttctgtag tctggctgct ctctatccgt cattagttgt
    10921 aacgcccttt cgaaatggct acgcgatgaa tcagtgccat tatcaactgc gttctgaaca
    10981 ctttcaattt cgttatcatt tgaaatagga gtaatacaac catttattat ggtataacca
    11041 acgccatgct ttttaaagat ggaattgaga tgcttcgata gattaatata tgaattagtt
    11101 ctctcaatga tgaactcaat taaatcatat accaaatacc atgcttcccc atatatataa
    11161 tctcggatag cagtcagcaa cgtcttatca cttttgtatc cactttcata acgaggaata
    11221 ttatccgcag gttgatttag ataatatatc cacacagact gcgcacattt tgttgctgta
    11281 gcagtttgac gattgttagt ccaaaggaaa agatataagc aattccacaa tgccatgcgt
    11341 gtatcagaat taagatcatt cagctggaca tgctctctaa cgtcaacatg accatacctc
    11401 acagaaaatg gctttatcat tcttcccctg cctgattgtt atgttcgttt aaattatgca
    11461 gtctaagttt gatacaatca tatattaagc aatagaatcc ataaagcccc gttggtggta
    11521 agacaacaag cggagcctct tgcgacttca tcagggttat gtcagttagt gaggcttatc
    11581 aggatgacca tgtacccaag gggagcgagg ccgaaccgct ggcagagctt gcatatggtc
    11641 tgcaagtacc gtgaccacca taaacctgtt tatcccctgc ggcgtctcgc gacggcgaca
    11701 gaatgggggc atcgggctaa aatgcgctgc actaaaagtt ttgggattgt ttagagacgg
    11761 tggctacgtc atagctttca agttgagtca gtacgtgttt gacgcttgga acgacacgcc
    11821 gctatgagtt aacatcccaa ttaccactat caaccacagc ggctttttca agggacataa
    11881 cagagcattt cttacaggca aaagagacag atttaatcgt ctaactaact gtattgtaag
    11941 gaatttagta acttggtgcc gaaggccgga ctcgaaccgg cacgtatttc tacggttgat
    12001 tttgaatcaa ctgcgtctac cgatttcgcc acttcggcac tgaagaggta tgcggaaaac
    12061 gttgtggatt atacctgtcg cacgccacca tgcaagcgcc agcttgcacc cacctcgcta
    12121 agtgctgaaa ttttcagcat tacactcagc gatatgtaac ctttgtcaca ctccaggcac
    12181 cccgccctgc catgctctac acttcccaaa caacaccaga gaaggaccca aaaatgtcga
    12241 tgataaaaag ctatgccgca aaagaagcgg gcggcgaact ggaagtttat gagtacgatc
    12301 ccggtgagct gaagccacaa gatgttgaag tgcaggtgga ttactgcggg atttgtcatt
    12361 ccgatctgtc gatgatcgat aacgaatggg gattttcaca atatccgctg gttgccgggc
    12421 atgaggtgat tgggcgcgtg gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg
    12481 tcggtcagcg tgtcgggatt ggctggacgg cgcgtagctg tggtcactgc gacgcctgta
    12541 ttagcggtaa tcagatcaac tgcgagcaag gtgcggtgcc gacgattatg aatcgcggtg
    12601 gctttgccga gaagttgcgt gcggactggc aatgggtgat tccactgcca gaaaatattg
    12661 atatcgagtc cgccgggccg ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga
    12721 tgcaccatat cactgctacc agccgcgttg gggtaattgg tattggcggg ctggggcata
    12781 tcgctataaa acttctgcac gcaatgggat gcgaggtgac ggcctttagt tctaatccgg
    12841 cgaaagagca ggaagtactg gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc
    12901 aggcactgaa agccctgtcg gggcagtttg atctcattat caatactgtg aacgtcagcc
    12961 tcgactggca gccttatttt gaggcgctga cctatggcgg taatttccat acggtcggtg
    13021 cggttctcac gccgctgtct gttccggcct ttacgttaat tgcgggcgac cgcagcatct
    13081 ctggttctgc taccggcacg ccttatgagc tgcgtaagct gatgcgtttt gccgcccgca
    13141 gcaaggttgc gccgaccacc gaactgttcc cgatgtcgaa aattaacgac gccatccagc
    13201 atgtacgcga tggtaaagcg cgctaccgcg tggtcctgaa agccgacttc tgatcccttt
    13261 gccgggtgcc tgtcgcccgg cacttaaatc tctttggaag gcgtctcgca atctgctgaa
    13321 tttagcggtg aagagtgcac agccgttgcc tatactccag gcaggaaact ggaggaaatc
    13381 tcatgagcga acccctgtta attgcccgca cgccggacac agaactgttt ttactgccgg
    13441 gaatggctaa ccgtcacggg ctgattactg gcgcaacggg gacgggtaaa accgttacgc
    13501 tgcaaaaact ggcagagtca ttgtcggaaa tcggcgtgcc ggtgtttatg gctgatgtga
    13561 aaggcgatct gaccggtatc gcgcaggcag gaacggcgtc ggaaaaactg ctcacaaggc
    13621 ttaaaaatat cggcgtcaat gactggcaac cgcatgccaa tccggtggtg gtttgggata
    13681 tctttggcga gaaaggccat ccggtgcggg cgacggtttc ggatctgggg ccgctgttgc
    13741 tggcgcggct gttgaatctc aacgatgtgc agtctggcgt gctgaatatc atcttccgca
    13801 ttgctgacga tcaggggctg ttactgctcg actttaaaga tttgcgggcg attacccagt
    13861 acatcggcga taacgccaaa tctttccaga atcagtacgg aaatatcagt agcgcatcgg
    13921 ttggtgccat ccagcgcggg ctgttgtcgc tggaacagca aggcgcagca cacttctttg
    13981 gtgagccgat gctggatatc aaagactgga tgcgcaccga tgccaacggt aaaggcgtta
    14041 tcaatatcct cagcgccgag aagctttatc agatgccgaa actgtatgcc gccagcctgt
    14101 tgtggatgct ctcggagttg tatgaacaat tgccggaagc gggcgatctg gagaaaccaa
    14161 aactggtgtt tttcttcgac gaggcacatc tgctgtttaa cgatgcaccg caggtactgc
    14221 tggataagat tgagcaggtg ataaggctta ttcgctcaaa aggcgtgggc gtctggttcg
    14281 tttcgcaaaa tccgtccgat attccggata acgtgctcgg ccagctcggt aatcgcgttc
    14341 aacacgcttt gcggactttt acgcccaaag atcagaaagc ggtaaaagct gcggcgcaaa
    14401 ccatgcgggt caatccggca tttgataccg aaaaggcgat tcaggaactg ggcaccggcg
    14461 aggcgttgat ctcttttctg gatgcgaaag gaagcccttc tgtggtggaa cgggcgatgg
    14521 tgattgcgcc ttgttcgcgg atgggaccgg tgacggaaga tgagcgtaat ggcttgatta
    14581 atcactctcc ggtgtatggc aagtacgagg atgacgtgga ccgggaatcc gcctatgaga
    14641 tgttgcaaaa aggcgtacag gcaagcgccg agcagcaaaa taatcctgcc gtgaaaggga
    14701 aagaggtggc ggtggatgac ggcattcttg gtggattgaa ggatattttg tttggcacta
    14761 ccggaccacg cggcgggaag aaagatggtg tggtgcaaac aatggcgaaa agcgccgccc
    14821 gccaggtgac gaatcagatt gtacgtggga tgttggggag tttgctgggg ggaagaagaa
    14881 ggtaagtcag gcgcgatttg tctttgcctg atgcgacgct ggcgcgtctt atcatgccta
    14941 caccactgca atatattgga ttttcatgat ttttcaggca tgaacgaagc acactttgct
    15001 aacaataata aagggagctt tcgctccctt tattcgttca ttcggttacg attttctcat
    15061 taacagccac aggctgatta agaagaagct ggcgcttggc aacagtgcgc cgatgatcgg
    15121 cgggatgcca taaaccaacg tcagcgggcc gaagatctgg tccagtacat agaagacaaa
    15181 accgaagctg ataccggtga ccacacgcac acccatcggt acgctacgca gtgggccaaa
    15241 gatgaacgac agcgccatca gcatcatcac cgccacggat agaggctgga agattttgct
    15301 ccacatgttg agctgataac gtccggcatc ctgaccgctc gatttcagat acttcacata
    15361 gttgtgcaaa ccgctgatag agagtgcatc cggatccagc gccaccacgc ccagtttgtc
    15421 aggcgtgagg ttggttttcc aggtgccgct caccgtctgc gaaccggtaa tctgtttcgg
    15481 attggtcaga tcagattcat caacctgcga cagacgccag actttatgtt ccgggtcaaa
    15541 cttcgcagta gcggcatagc gtacggattg cagacgacga ttttcgttaa aggcataaat
    15601 gctgatgcca cctaactctt cgtcaccttt aacccgctca atgtagacga agttgttgcc
    15661 atctttcgcc cataagcctt gctgggtaga gagcaacgag ccgccgtaca tcgcctgcgc
    15721 acggtagtta cgcgccatct gctcgccctg cggcgcgacc cattcgccaa tcgccatcgt
    15781 cagcaagacc agcggaatgg cggttttcat caccgacagc gccacctgca tacgggtaaa
    15841 accagaagcc tgcatcacca ccagttcgct gcgctgcgcc agcatcccaa gaccaagcaa
    15901 cgccccaagc agagccgcca tcgggaagaa gatttgcaca tctttcggca cgctcagcaa
    15961 ggtatacatt cctgcgccta acgcgtcgta actcccctgc ccggcttttt tcaactgatc
    16021 gacaaatttg ataatgcccg acagcgacac cagcatgaac agcgtcatca tgatggtggt
    16081 gaaaatagtt ttaccgatat agcggtcaag tacgccaaaa ggttgcatca caccgctcct
    16141 ttacgcgaaa aactggcgcg caggcggcgg accggcacgg tgtcccaaag gttgagaaca
    16201 atcgccaaag ccagataaat caggttaacg gtccacatcc acagcgtcgg gtccagctta
    16261 cctttaccgc cgttcgattt cagggaggtc tggatcagga agaaaagtag atacagcagc
    16321 atggctggca gcatcgacag tacgcgtccc tgacgtgggt taaccacgct cagcggtacg
    16381 accataagtg ccatcataaa cacggtgaat accaacgtga tacgccagtt cagttctgcg
    16441 cgagcacgat cggtgtcagt gttccacaat gtgcacatgt ccatctggtc ggtatcgttc
    16501 gggtcgagcg ccaccgcctg gtgaccaatg atcgcctgat aatcctggaa atccgtaatg
    16561 cggaaatcac gtaacagtgc agtgccttcg aagcgcgttc cctggttgag agtgacgacc
    16621 tgggagccgt cgcgcagctg ggttaaatgt ccggaatcgg ccaccaccac agaaggacgt
    16681 gcattacctt ttggtcgaat ttgtgcgagg aacacatctt tgaaatcgct gccgtcaacg
    16741 ctttcgatga acagcaccga gctgccatta gtcgcttgct ggaattgccc ttgcgccagc
    16801 gccgccatgc cagggttcgc tttcgcttct gctaacactt catcctgatg acgcgatgac
    16861 cacggtcccg cccacatcac gttaaccgcc gcgacgattg ccgtgaatac cgcaaggatc
    16921 attgccgctt tcaccagaac cgctttgctc aggccgcagg catgcattac cgtaatttca
    16981 ctttcggtat acagtttgcc cagcgtcatc agcagcccga ggaacaggct taatggcagg
    17041 ataagctgcg ccatttccgg cacgcccaac ccgagaaggg agagcaccag attcgccgga
    17101 atatcgccgt caaccgctgc gccgaggatc ctcactaact tttgacagaa gaagatcaaa
    17161 agcaagatga agagtatcgc cagctggctt ttgagcgtct cccgcaccag atatcttatg
    17221 attatcactt taaatacgcc cgtaaaaact cgtcttttgc aggattttag cttgtttcat
    17281 ggcttaaacg tcatttattc tcttgagtcg tcgaaatcgt cgctaagata attatactca
    17341 acggattcac ctctcagatt ttgttctgac gtgccaatgc cgtaataacg ttaagattaa
    17401 cacgaagtca tcgcaacagc ggacatgagt tacgaaagct tgcaattcta tctgtagcca
    17461 ccgccgttgt ctttaagatt caggagcgta gtgcatggag tttagtgtaa aaagcggtag
    17521 cccggagaaa cagcggagtg cctgcatcgt cgtgggcgtc ttcgaaccac gtcgcctttc
    17581 tccgattgca gaacagctcg ataaaatcag cgatgggtac atcagcgccc tgctacgtcg
    17641 gggcgaactg gaaggaaaac cggggcagac attgttgctg caccatgttc cgaatgtact
    17701 ttccgagcga attctcctta ttggttgcgg caaagaacgt gagctggatg agcgtcagta
    17761 caagcaggtt attcagaaaa ccattaatac gctgaatgat actggctcaa tggaagcggt
    17821 ctgctttctg actgaactgc acgttaaagg ccgtaacaac tactggaaag tgcgtcaggc
    17881 tgtcgagacg gcaaaagaaa cgctctacag tttcgatcag ctgaaaacga acaagagcga
    17941 accgcgtcgt ccgctgcgta aaatggtgtt caacgtgccg acccgccgtg aactgaccag
    18001 cggtgagcgc gcgatccagc acggtctggc gattgccgcc gggattaaag cagcaaagga
    18061 tcttggcaat atgccgccga atatctgtaa cgccgcttac ctcgcttcac aagcgcgcca
    18121 gctggctgac agctacagca agaatgttat cacccgcgtt atcggcgaac agcagatgaa
    18181 agagctgggg atgcattcct atctggcggt cggtcagggt tcgcagaacg aatcgctgat
    18241 gtcggtgatt gagtacaaag gcaacgcgtc ggaagatgca cgcccaatcg tgctggtggg
    18301 taaaggttta accttcgact ccggcggtat ctcgatcaag ccttcagaag gcatggatga
    18361 gatgaagtac gatatgtgcg gtgcggcagc ggtttacggt gtgatgcgta tggtcgcgga
    18421 gctacaactg ccgattaacg ttatcggcgt gttggcaggc tgcgaaaaca tgcctggcgg
    18481 acgagcctat cgtccgggcg atgtgttaac caccatgtcc ggtcaaaccg ttgaagtgct
    18541 gaatactgac gctgaaggcc gcctggtact gtgcgacgtg ttaacttacg ttgagcgttt
    18601 tgagccggaa gcggtgattg acgtggcgac gctgaccggt gcctgcgtga tcgcgctggg
    18661 tcatcacatt accggtctga tggcgaacca taatccgctg gcccatgaac tgattgccgc
    18721 gtctgaacaa tccggtgacc gcgcatggcg cttaccgctg ggtgacgagt atcaggaaca
    18781 gctggagtcc aattttgccg atatggcgaa cattggcggt cgtcctggtg gggcgattac
    18841 cgcaggttgc ttcctgtcgc gctttacccg taagtacaac tgggcgcacc tggatatcgc
    18901 cggaaccgcc tggcgttctg gtaaagcaaa aggcgcaacc ggtcgtccgg tagcgttgct
    18961 ggcacagttc ctgttgaacc gcgctgggtt taacggcgaa gagtaattgc gtcaggcaag
    19021 gctgttattg ccggatgcgg cgtgaacgcc ttatccgacc tacacagcac tgaactcgta
    19081 ggcctgataa gacgcgcaag cgtcgcatca ggcaatgctg ttattgtcgg atgcggcatg
    19141 aacgccttat ccgacctaca cagcaccgaa ctcgtaggcc tgataagacg cggcagcgtc
    19201 gcatcaggca atgctgttat tgtcggatgc ggcgtgagcg ccttatccga cctacacaca
    19261 gcactgaact cgtaggcctg ataagacaca acagcgtcgc atcaggcgct gcggtgtata
    19321 cctgatgcgt atttaaatcc accacaagaa gccccatata tgaaaaacgc gacgttctac
    19381 cttctggaca atgacaccac cgtcgatggc ttaagcgccg ttgagcaact ggtgtgtgaa
    19441 attgccgcag aacgttggcg cagcggtaag cgcgtgctca tcgcctgtga agatgaaaag
    19501 caggcttacc ggctggatga agccctgtgg gcgcgtccgg cagaaagctt tgttccgcat
    19561 aatttagcgg gagaaggacc gcgcggcggt gcgccggtgg agatcgcctg gccgcaaaaa
    19621 cgcagcagca gcccgcgcga tatattgatt agtctgcgaa caagctttgc agattttgcc
    19681 accgctttca cagaagtggt agacttcgtt ccttatgaag attctctgaa acaactggcg
    19741 cgcgaacgct ataaagccta ccgcgtggct ggtttcaacc tgaatacggc aacctggaaa
    19801 taatggaaaa gacatataac ccacaagata tcgaacagcc gctttacgag cactgggaaa
    19861 agcagggcta ctttaagcct aatggcgatg aaagccagga aagtttctgc atcatgatcc
    19921 cgccgccgaa cgtcaccggc agtttgcata tgggtcacgc cttccagcaa accatcatgg
    19981 ataccatgat ccgctatcag cgcatgcagg gcaaaaacac cctgtggcag gtcggtactg
    20041 accacgctgg gatcgctacc cagatggtcg ttgagcgcaa gattgccgca gaagaaggta
    20101 aaacccgtca cgactacggc cgcgaagctt tcatcgacaa aatttgggaa tggaaagcgg
    20161 aatccggcgg caccattacc cgtcagatgc gccgtctcgg caactccgtg gactgggagc
    20221 gtgaacgctt cacgatggac gaaggcctgt ccaatgcggt gaaagaagtt ttcgttcgtc
    20281 tgtataaaga agacctgatt taccgtggca aacgcctggt aaactgggac ccgaaactgc
    20341 gcaccgctat ctctgacctg gaagtggaaa accgcgaatc gaaaggttcg atgtggcaca
    20401 tccgctatcc gctggctgac ggtgcgaaaa ccgcagacgg taaagattac ctggtggttg
    20461 cgactacccg tccggaaacc ctgctgggcg ataccggtgt agccgttaac ccggaagatc
    20521 cgcgttacaa agacctgatt ggcaaatatg tcattctacc gctggttaac cgtcgtattc
    20581 cgatcgttgg cgacgaacac gccgacatgg aaaaaggcac cggctgcgtg aagatcaccc
    20641 cggcgcacga ctttaacgac tatgaagtcg gtaaacgtca cgccctgccg atgatcaaca
    20701 tcctgacctt tgacggcgat atccgtgaaa gcgcccaggt gttcgatacc aaaggtaacg
    20761 aatctgacgt ttattccagc gagatcccgg cagaattcca gaaactggag cgttttgccg
    20821 cacgtaaagc agtcgttgct gcggttgacg cactcggcct gctggaagaa attaaaccgc
    20881 acgacctgac cgttccttac ggcgaccgtg gcggcgtagt tatcgaacca atgctgaccg
    20941 accagtggta cgtgcgtgcc gatgtactgg cgaaaccggc ggttgaagcg gttgagaacg
    21001 gcgacattca gttcgtaccg aagcagtacg aaaacatgta tttctcctgg atgcgcgata
    21061 ttcaggactg gtgtatctct cgtcagctgt ggtggggtca ccgtatcccg gcatggtatg
    21121 acgaagcggg taatgtttat gttggccgca acgaagaaga agtgcgcaag gaaaataacc
    21181 tcggtgccga cgttgccctg cgtcaggacg aagacgttct cgacacctgg ttctcttctg
    21241 cgctgtggac cttctctacc cttggctggc cggaaaacac cgacgccctg cgtcagttcc
    21301 acccaaccag cgtgatggta tccggcttcg acatcatctt cttctggatt gcccgcatga
    21361 tcatgatgac catgcacttc atcaaagatg aaaatggcaa accgcaggtg ccgttccaca
    21421 ctgtttacat gaccggtctg attcgtgacg acgaaggcca gaaaatgtcc aaatccaagg
    21481 gtaacgttat cgacccgctg gatatggttg acggtatttc gctgccagaa ctgctggaaa
    21541 aacgtaccgg caatatgatg cagccgcagc tggcggacaa aatccgtaag cgcaccgaga
    21601 aacagttccc gaacggcatt gagccgcacg gcaccgatgc cctgcgcttc accctggcgg
    21661 cgctggcgtc taccggtcgt gacatcaact gggatatgaa gcgcctggaa ggttaccgta
    21721 acttctgtaa caagctgtgg aacgccagcc gctttgtgct gatgaacaca gaaggtcagg
    21781 attgcggctt taacggcggc gaaatgacac tgtcgctggc ggaccgctgg attctggcag
    21841 agttcaacca gaccatcaaa gcgtaccgcg aagcgctgga cagcttccgc ttcgatatcg
    21901 ctgcaggcat tctgtatgag ttcacctgga accagttctg tgactggtat ctggagctga
    21961 ccaagccggt aatgaacggt ggcaccgaag ccgaactgcg cggtactcgc catacgctgg
    22021 tgactgtact ggaaggtctg ctgcgcctcg cgcatccgat cattccgttc atcaccgaaa
    22081 ccatctggca gcgtgtgaaa gtactttgcg gtatcactgc cgacaccatc atgctgcagc
    22141 cgttcccgca gtacgatgca tctcaggttg atgaagccgc actggccgac accgaatggc
    22201 tgaaacaggc gatcgttgcg gtacgtaaca tccgtgcaga aatgaacatc gcgccgggca
    22261 aaccgctgga gctgctgctg cgtggttgca gcgcggatgc agaacgtcgc gtaaatgaaa
    22321 accgtggctt cctgcaaacc ctggcgcgtc tggaaagcat caccgtgctg cctgccgatg
    22381 acaaaggtcc ggtttccgtt acgaagatca tcgacggcgc agagctgctg atcccgatgg
    22441 ctggcctcat caacaaagaa gatgagctgg cgcgtctggc gaaagaagtg gcgaagatcg
    22501 aaggcgaaat cagccgtatc gagaacaagc tggcgaatga aggctttgtc gcccgcgcac
    22561 cggaagcggt catcgcgaaa gagcgtgaga agctggaagg ctatgcggaa gcgaaagcga
    22621 agctgattga acagcaggct gttatcgccg cgctgtaatc aacaccgtga tgaaaacgaa
    22681 ggccggagaa tgctccggcc ttttttatct cttacagaaa tggtaaagaa ggcataattc
    22741 cccgtgaaat actggctaag aagcctttat ctggttgttc ttctttattg attaaaggaa
    22801 ggtcatccaa atcgcccagc aaatacgtat tttttgcctg ccagtcaatc atccatattt
    22861 tcagcagtgg ataagccaga ccgcccgtaa tcccggatat cactaccaac gcacacatgc
    22921 gataaagcat accgtggtaa gttaaagttg agcgaaaacg aatacgccca tcattcagtg
    22981 acaggttgct cataaaatga tttcgcaacg acaccgttaa ataactggtg ctaacagcaa
    23041 tcccaaaata atagattaac tgcgcgatta tcattttacg ttgcatttcc ataaattgct
    23101 gtaaattctc aatatcatca tttgcataca caaaactatc atacgcattt aatatttgat
    23161 cgaagataat ataaccagca acagcgagaa aaggtaataa agctaaaaat gcgagaatgg
    23221 catattttat acaatacgta gtatctaatt tcaccttgaa acgatgtata ccgaaactgg
    23281 tattgctcca gagaaaactc attaccagac tatataaagt accattaaaa ataccaatgg
    23341 aaacaatacc aactattgcc atcagaacca cagatataat aacactactt gaactattgg
    23401 catgtagcat ctttgtcgag ataaagaaaa cagtccccat cccaatggcc attaaaatcg
    23461 gcaagaaaaa ggttacccac cagaaccctt tcaaagagca attaaaacta aatcttacgc
    23521 cgttgagact agtcatcaag gcctgataac gcagtccttt tgctgccata aatataagca
    23581 aaaccaacag tgacaaagtc aaaacacagt caacaagcgg catatctgct gacactgtca
    23641 ttaagattgc gaaatagaaa aaaacaaaaa caagacaact aacaaaaaca ttcccaccgg
    23701 taatcccata agaaaatcgt tgtccgttaa cttccatatt agcatagaga taacgcttac
    23761 atttcattaa tgcccatggt aaatagatac ctagcgttat aatcgttaac aacacattca
    23821 ccacacaaat aagaaaatat ctctcacctg taccatgaaa aacaaacgaa tgggacggaa
    23881 catccatttc attaataact tgagccataa aaattccctg aattaaaaaa tccattgata
    23941 taatttatag atacagacag taaaatgttc tgccacgtta caatactctt atcagtaata
    24001 ggctggcaaa aactttattg ctttataaga cgcttgcgca cgtaagaaga gagtggtatt
    24061 aaacaaatgt ataattactt tatttttatt gggttaaact atgaataaca ttgcgccgca
    24121 atcacctgta atgcgtcgcc tgacgttgca ggataatcct gctatcgccc gcgtcattcg
    24181 tcaggtatcc gccgaatacg gtcttaccgc tgataaaggc tacaccgtcg ccgatccgaa
    24241 tcttgacgag ctgtatcaag tgtatagtca gcctggtcat gcatattggg tggttgagta
    24301 cgagggtgaa gtggtcggcg gcggtgggat tgcgccatta gcaggtagtg aatcggacat
    24361 ttgcgaactg caaaagatgt attttcttcc ggctattcgc ggtaaaaggc tggcgaaaaa
    24421 actggcctta atggcgatgg agcaagcacg agagatgggc ttcaaacgct gctatctgga
    24481 aacgaccgct tttttaaagg aagccattgc gctttatgag catttgggct ttgagcatat
    24541 cgactatgcg cttggctgca cgggccatgt cgattgtgaa gtgcggatgc tacgcgaact
    24601 ctaaaaaaat gcccgctagt gtgtgctatc gggcatttta atcaattaat tagtggcgaa
    24661 ccccgtcatc gtcttcatcg acaaaatctt catcgtcgcc gtcttcgcca ttgggatctt
    24721 caaagtaagt gccccaaccg tcgtactcaa cgtcaaattt ctccgccagc gtcatcagtt
    24781 gttcaacctg ggcatcgatc agatcggcat tcaacgcgca ctcgctgagg atgtcgcagc
    24841 aaatcacgat atcaccatct tcaacttcca gctcttctgg atcggtcact tcgtaaccga
    24901 gtttaaacgc ttcaactgct gctttttcca gggtttccag atcgtctgcg gaaagatggt
    24961 gttcgatggt gtacagcgcg tccggatcgc tgccatcttc cagtaattct tcaataatca
    25021 aacgtgtttc ttcacgctgt tcttccagtt gttccgggtt tgccatggct cattcctctt
    25081 aatgtcctgc cgatactctt attgtcacac accactgaca ttgcctccac ctttgtgaca
    25141 aagatttatg ctttagactt gcaaatgaat aatcatccat ataaattgaa ttttaattca
    25201 ttgagacgtt agccacagga gggatctatg tccgggtttt atcataagca tttcctgaaa
    25261 ttactcgatt tcacgccagc tgaactcaac agcctgttgc agttagccgc gaagctgaaa
    25321 gccgataaga aaagcggtaa agaagaagcc aaactcactg gtaaaaacat cgcgctcatc
    25381 ttcgaaaaag actcgactcg tacccgatgc tctttcgaag ttgccgcata tgaccagggt
    25441 gctcgtgtta cttatctcgg cccaagcggc agccagattg gtcataaaga gtcgattaaa
    25501 gacactgccc gcgtgcttgg tcgcatgtat gacggtattc agtatcgcgg ctatggtcag
    25561 gagattgtcg aaacactggc ggaatacgct ggcgtgccgg tatggaacgg cctgaccaat
    25621 gagtttcacc ccacgcagct gctggcggat cttctcacca tgcaggagca tttgcccggc
    25681 aaagcattca acgaaatgac gctggtctat gcaggtgacg cgcgtaacaa catgggaaac
    25741 tcgatgctcg aagctgcggc gcttaccggt ctggatttgc gtctggtcgc gccacaggca
    25801 tgctggccgg aagctgcgct ggttacggaa tgccgcgccc tggcacagca aaatggcggg
    25861 aatattacgc tgacggaaga tgtagccaaa ggagtagaag gtgctgactt tatctatacc
    25921 gatgtatggg tgtcgatggg tgaagcaaaa gagaaatggg cggaacggat tgcattgctg
    25981 cgtgattatc aggtgaataa caagatgatg cagttgaccg gtaacccgga ggtcaaattc
    26041 ctccactgcc tgcccgcgtt tcatgacgac cagacgacgc ttggtaagaa aatggcggaa
    26101 gagtttggcc tacatggcgg aatggaagtg actgatgagg tcttcgaatc tgccgccagc
    26161 attgtttttg atcaggcgga aaaccgtatg cataccatca aagcggtgat ggtcgcgacg
    26221 ctcagtaaat aattgatatc gctataaaga tgccgggaaa tacaaaacta tcccggcatc
    26281 acattatttt atgtagtaat agttctattc taccacccaa atttttctat tactatacgt
    26341 aaatcttccg gaggatttaa ttttcccaac tgttcattat taatatattc atatattcat
    26401 atattc
//
LOCUS       DAJOSY010000062        25189 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJOSY010000062 DAJOSY010000000
VERSION     DAJOSY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.062"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1236)
                     /locus_tag="OHN27_002671"
     CDS             complement(<1..1236)
                     /locus_tag="OHN27_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCQ2287080.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYP"
     gene            complement(1349..3283)
                     /gene="yeaG"
                     /locus_tag="OHN27_002672"
     CDS             complement(1349..3283)
                     /gene="yeaG"
                     /locus_tag="OHN27_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCQ2287081.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3719..4465
                     /gene="mipA"
                     /locus_tag="OHN27_002673"
     CDS             3719..4465
                     /gene="mipA"
                     /locus_tag="OHN27_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCQ2287082.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4555..5409
                     /gene="yeaE"
                     /locus_tag="OHN27_002674"
     CDS             4555..5409
                     /gene="yeaE"
                     /locus_tag="OHN27_002674"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCQ2287083.1"
                     /translation="MQQKMIQFSGDVSLPAVGQGTWYMSEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5457..6341)
                     /locus_tag="OHN27_002675"
     CDS             complement(5457..6341)
                     /locus_tag="OHN27_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCQ2287084.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGIAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6425..7420)
                     /gene="gapA"
                     /locus_tag="OHN27_002676"
     CDS             complement(6425..7420)
                     /gene="gapA"
                     /locus_tag="OHN27_002676"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCQ2287085.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7762..8175
                     /gene="msrB"
                     /locus_tag="OHN27_002677"
     CDS             7762..8175
                     /gene="msrB"
                     /locus_tag="OHN27_002677"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCQ2287086.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8217..8489
                     /locus_tag="OHN27_002678"
     CDS             8217..8489
                     /locus_tag="OHN27_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCQ2287087.1"
                     /translation="MNLDDIINSMMPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNIEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            8859..9935
                     /locus_tag="OHN27_002679"
     CDS             8859..9935
                     /locus_tag="OHN27_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCQ2287088.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPMCGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            9962..11341
                     /locus_tag="OHN27_002680"
     CDS             9962..11341
                     /locus_tag="OHN27_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2287089.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRINGIAAPYAVAVLLSSYGVTGVFILLGAVSIVVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11358..12401
                     /locus_tag="OHN27_002681"
     CDS             11358..12401
                     /locus_tag="OHN27_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HCQ2287090.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVI
                     NGTKEDTISRCQQFTEDMGADIVFETAGSAVTIKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12422..13258
                     /locus_tag="OHN27_002682"
     CDS             12422..13258
                     /locus_tag="OHN27_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCQ2287091.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13263..14210
                     /locus_tag="OHN27_002683"
     CDS             13263..14210
                     /locus_tag="OHN27_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCQ2287092.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFSRFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KTRQMIICADMIKPRLNETLDDICEALSYVDYLFPNYTEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14220..15200
                     /gene="ydjG"
                     /locus_tag="OHN27_002684"
     CDS             14220..15200
                     /gene="ydjG"
                     /locus_tag="OHN27_002684"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCQ2287093.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALIINLSDADATLMREMAEALER"
     gene            15337..16095
                     /locus_tag="OHN27_002685"
     CDS             15337..16095
                     /locus_tag="OHN27_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2287094.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16212..17570
                     /locus_tag="OHN27_002686"
     CDS             16212..17570
                     /locus_tag="OHN27_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2287095.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(17663..18304)
                     /gene="pncA"
                     /locus_tag="OHN27_002687"
     CDS             complement(17663..18304)
                     /gene="pncA"
                     /locus_tag="OHN27_002687"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCQ2287096.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(18315..19331)
                     /gene="ansA"
                     /locus_tag="OHN27_002688"
     CDS             complement(18315..19331)
                     /gene="ansA"
                     /locus_tag="OHN27_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCQ2287097.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(19498..21354)
                     /gene="sppA"
                     /locus_tag="OHN27_002689"
     CDS             complement(19498..21354)
                     /gene="sppA"
                     /locus_tag="OHN27_002689"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCQ2287098.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAEQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            21515..22066
                     /locus_tag="OHN27_002690"
     CDS             21515..22066
                     /locus_tag="OHN27_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCQ2287099.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            22183..23226
                     /gene="selD"
                     /locus_tag="OHN27_002691"
     CDS             22183..23226
                     /gene="selD"
                     /locus_tag="OHN27_002691"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCQ2287100.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            23231..25177
                     /gene="topB"
                     /locus_tag="OHN27_002692"
     CDS             23231..25177
                     /gene="topB"
                     /locus_tag="OHN27_002692"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCQ2287101.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQARSTPVRQFRGIVAPGGES
                     VKKAPNKRVKKAAKPQKDNTDSAN"
BASE COUNT         6333 a         5899 c         6491 g         6466 t
ORIGIN      
        1 cggataaata tcatcctggt cgcggatgtg ctgcatcgca aagttgtcga aagtagattg
       61 cagatgctca tattctcgcc acaatgtctg atgtgcacga cgggtaattt cgatatagct
      121 gtaataacga acaacaggta ataatttttt cgccaggatt tcatggcaaa gcggagagtc
      181 atcggcccag ttatcgccgt ccgatgcttg tgcagcgtaa atattccact gtgccgggtt
      241 ataacgctct ttcactacct catccatcag tttcagggcg ctggaaacaa tggtgccgcc
      301 tgtttcctgc gagtagaaaa actcatgttc atcgacttct ttcgcctggg tatgatggcg
      361 gatgtatacg acttccacgt tcttatacgt tctgctgagg aacagataca gcagaatata
      421 aaaacgctta gccatatctt tagtggattg atccattgaa ccggaaacgt ccatcaggca
      481 aaacatcact gcctggctgg agggatcggg ccgcttctcg tagttcttgt aacgtaaatc
      541 gaaggtgtca ataaaaggga cgcgttcaat tttggcacgt aattctgcaa tttctttgcg
      601 cagacgttcc tcttccagca gttgcgcagg ttcactgttg ctgatgatgg ccaaattctc
      661 ttccagtgca tgaagttccc gccgcttgcc tgccgtcatg gctgtgcgtc gcgccagtga
      721 gttctgcaat gaacgcacaa cgctgatatt ggccggaacg ccgttagcgg tataacccgc
      781 ccgatgcgtt ttatattcgg tcagctggcg ttgttggttt tgtttcagat tcggtaaggc
      841 caaatcttca aagagcagat caagatactc atctttcgaa atctgaaaga caaattcatc
      901 ctgaccttca ccatcctggc tggcctggcc ctgaccactg ccggaacctc cgccgccacc
      961 ctggggacgt tcaattcggt cgttctggac gaaatggtca ttgcccggat gcacgcggtg
     1021 gcgcagaccg ccacgcccct gatgaaacat cggttcgcta atatcttccg tgggaatgga
     1081 tacggattcg ccgctgtcga cgtcagtcac cgaacgctta ttaatggcct cggagatcga
     1141 ctgtttaatt tgcgctttat aacggcgtaa aaaacgctgg cgattcacca tgcttttgtt
     1201 tttgccgttc agacgccggt caataaacca ggtcatatgc cccccgtact acatttgcca
     1261 actgtatgct acgcctgtgt tgccgcttct caggcccacg gttcgctgtc gttgtaggcc
     1321 cggtaacatg tgtaccgggc cagggttgtt aagacgattt acgtacgcgc aaataccatt
     1381 cgcacagtaa acgcacctgt ttacgggtgt agcctttttc catcatacgg tcgacaaagt
     1441 cgtcgtgttt cttctgctcg tcggttgacg ttttggcgtt aaacgagata accggcaaca
     1501 gctcctcggt attggagaac attttcttct cgatgaccgt gcgcagtttt tcatagctgg
     1561 tccagttcgg attgcgtccg ctgttattcg ctctggcgcg cagtacgaag ttaacaatct
     1621 cgttgcggaa atcttttgga ttactgatcc ccgccggttt ctcgattttc tccagctcgg
     1681 cgttaagaga ctcgcggtca aacagctgcc cggtatccgg atcgcgatac tcctgatcct
     1741 gaatccagaa atccgcgtag gtaacataac ggtcgaaaat gttttgccca tattcggaat
     1801 aggattcaag gtaggccgtc tggatctctt tgccgataaa ctcggcatat ttcgggatca
     1861 gataaccttt caggaactcc agatagcgtt ctgcctgctc ttgtgggaac tgctcgcgct
     1921 cgatctgctg ttccaggacg tagaacagat ggaccgggtt tgccgccact tctacatgat
     1981 cgaagttgaa cacgcgggag aggatcttaa acgcaaaacg cgtcgacaga ccgttcatcc
     2041 cttcatcgac accggcgtag tcacgatatt cctgatacga cttggctttg ggatcggtgt
     2101 ctttcagact ttcgccatca taaacccgca tctttgaata aatgctggag ttttctggct
     2161 ctttaaggcg cgaaagaatg gaaaaacgtg acagcgtttc cagcgtgcca ggggcgcagg
     2221 gggcgtgagt caattcactg tgattaagca atttctcgta gattttgatc tcttcggaaa
     2281 tgcgtaagca atacggcacc ttcacgatgt aaacacggtc gaggaaggct tcgttgtttt
     2341 tgttattacg gaaagtgacc cattcggatt cgttcgagtg ggcgagaata atcccgttga
     2401 acggcagggc ggagatacct tccgtcccgt tgtagttacc ttcctgagtg gcggttaaca
     2461 gcggatgcag cacttttatc ggtgctttaa acatctcgac aaattccatg atcccttggt
     2521 tggcgcggca cagcgcaccg gaatagccgt aggcgtccgg gtcattctgc gcgtagtgtt
     2581 cgagtttacg aatatcgact ttcccaacca gcgcggagat gtcctggttg ttctcatcac
     2641 cgggttccgt tttggcgata gcaatttgtt gcagaattga cggccagacc ttcactaccc
     2701 ggaacttagt gatatcgcca ccaaattcat gcaggcgttt tgccgcccat ggcgacatga
     2761 tggtgccgag ataacggcga ggaatgccat actctttttc cagaatctga gcatcttcct
     2821 gcggattgaa aagacagaac ggatgatcgt tgaccgggct acgctcaccg ttggcgctca
     2881 atacataaat cggtacgagc tgcattaatg atttcagtcg ctcagcaagc gatgatttac
     2941 ccccacccac aggccccagc agatacagga tttgtttctt ctcttccagc ccctgagccg
     3001 cgtgtttcag ataagagaca atctgttcaa tcgcgtcttc catgccgtaa aactcttcaa
     3061 acgccggata acgtgcaatg acccggttag aaaagagtcg agaaagtctg ggttcctggg
     3121 ctgtatcgac catgacaggc tcaccgatag ccatcaatag ccgctcagcc gcgttggcat
     3181 aagcactgcg atcttgccga caagtggtaa gaaactcctg cagtgtgaac tcttcgtcct
     3241 tggcagcttc atatcgctgg cgatagtgat cgaatatatt catggtatgc cgtcctttcg
     3301 ttttttagca caggttaaga gccgttcata tgaatagtag aggctcccgg aagaggtaaa
     3361 ctgaacgacg tatcttgcta aaacacagca actctcatgc cattttatcg acgatgagaa
     3421 caacgtgccc ggtgatacac cttcaaaatt aagcgtagat ggcaaatgga aaacttgcct
     3481 gcacggcttt cttaatttca atggcatatc aacaactcat ccataaattt tgcataatta
     3541 atgtaaagac caggctcgcc agtaacgcta aattcatttg gctgtaagcg cggtgtcatc
     3601 cgcgtcagga aaattaaaca gttactttaa aaaatgaaaa cgtaaaaagg ttgggtttcg
     3661 atgtattgac gggtaaactt tgtcgcccgc taaacatttg tttaaggaat gattaattgt
     3721 gaccaaactc aaacttctgg cacttggagt gcttatcgca acgtctgcag gcgtagcgca
     3781 cgctgaaggt aaattttccc tgggcgcagg cgtaggtgtc gttgaacacc catataaaga
     3841 ttacgatacc gatgtttacc cagtaccggt aatcaactat gaaggcgata acttctggtt
     3901 ccgtggctta ggtggtggtt actacctgtg gaatgacgca acggataaac tttcaattac
     3961 cgcttactgg tcgccgcttt acttcaaagc gaaagacagt ggcgatcacc aaatgcgtca
     4021 cctggatgac cgtaagagca ccatgatggc tggtctgtct tatgctcact ttacccagta
     4081 cggttacctg cgtaccaccc tggctggcga taccctggat aacagcaacg gcatcgtctg
     4141 ggatatggcc tggttgtatc gttacaccaa cggtggcctg accgtgactc cgggtattgg
     4201 tgtgcagtgg aacagcgaaa accagaacga atactattat ggcgtatcgc gcaaagagtc
     4261 cgctcgcagc ggtctgcgtg gctataaccc gaacgacagc tggagccctt acctggagct
     4321 gagcgccagc tacaacttcc tcggcgactg gagtgtttac ggtaccgcac gctacacccg
     4381 tctgtctgat gaagttactg acagcccgat ggtggataaa tcctggactg gcctgatttc
     4441 taccgggatc acctacaaat tctgataatg catctttcca gggcattttg tgcataaaaa
     4501 cagggcgtca cgccctgttt tgcattacgg tggtgcaata cctgggagga aacaatgcaa
     4561 caaaaaatga ttcaatttag tggcgatgtc tcactgccag ccgtagggca gggaacatgg
     4621 tatatgagcg aagatgccag tcagcgcaaa acagaagttg ctgcactacg cgcgggcatt
     4681 gaactcggtt taaccctcat tgataccgcc gaaatgtatg ccgatggcgg tgccgaaaag
     4741 gtggttgggg aagcattaac cggtctgcga gagaaggtct ttctcgtctc taaagtctat
     4801 ccgtggaatg ctggcgggca aaaagcgata aatgcatgcg aagccagttt acgccgtctc
     4861 aatactgatt atctcgatct ttacttatta cactggtctg gcagtttcgc ttttgaagag
     4921 actgtcgcag cgatggaaaa attgatcgcc cagggaaaaa tccgccgctg gggcgtttct
     4981 aaccttgatt atgctgatat gcaggaactc tggcagctgc cggggggaaa tcagtgtgcc
     5041 actaatcagg tgctttacca tctcggttca cgaggaattg agtacgatct actcccctgg
     5101 tgccagcaac agcagatgcc ggtgatggct tacagtccgt tagcccaggc cgggcggttg
     5161 cgcaatggac tgttaaaaaa cgcggtagtc aacgaaattg cacatgctca caatatcagc
     5221 gcggcacaag tattgttggc gtgggtgatc agtcatcagg gtgtgatggc gattccaaaa
     5281 gcggccacga ttgcccatgt ccaacaaaat gcggctgtgc ttgaggtcga actttcttca
     5341 gcggaattag ctatgctgga taaggcatat ccggcaccaa aaggaaaaac tgcgctggat
     5401 atggtgtgat gttgtgtgct ggatgcatcg cacccggcac acaacattaa cgtaaattaa
     5461 cgtttcgcaa cgcgaatgga ttgcgccaga tgtgcaggtt tctctttggt cactttttgc
     5521 gtttctgaag cgtaagccgt ttctacacaa acaaatgttt tgtagccatc atccggcata
     5581 tcgcccatgc taattgaaag cgccggtccc gggttccagc cgacaacgtt cagatgatgc
     5641 tggtggccta cggcgataat acgattcagc gcttcatcat taatcacgct gcaatcttgt
     5701 ggattcagat acacgcgatc ggtacggtca gggaaggtct gaataccatc ggtcagtaca
     5761 tcttctttcg cgtcattcac tttatcaatg aagcgatcgc ccagcccact gacgcttacc
     5821 ttagcgatat cacccacgtt aaagtaggta tgcagggcag aggtggtttc aaattcgcca
     5881 tgtgattcaa gatcgatttc acaagtttta cccacgcgga aatgcgctaa cagcgtaaag
     5941 tcgtgcggcc agaatttttt cgtctcttcg ctttgcgtca attcaaaagt cagcgctacg
     6001 ccattagcat cttcacgatg tgatttcagc gtccacggca ggttgcgggc aaaaccgtgc
     6061 gcaggcaggc cttgttgtgc cgccggacca aaccacggcc agcaaaccgg tacgccaccg
     6121 cgaatagcga tgccattttt gaacggtgtg ttgttgctca accacagaac ttcttcttca
     6181 cccgcaggtt tccacgagag aaggtgtgcg ccctgtaatg caaaagaggc ttttacctgg
     6241 ggatgatcga ccacaatgag gtccagttca tccagtttac gacgggagag gacaggggag
     6301 atttgttcga tgaccggaag ggcaaaaatt ttcttaatca tgacgcagtc ctttaacttc
     6361 attttatcag gtaaaaaaaa gagcgaccga agtcgctctt tttagatcac agtgtcatct
     6421 caacttattt ggagatgtga gcgatcaggt ccagaacttt gttggagtaa ccggtttcgt
     6481 tgtcgtacca ggataccagt ttcacgaagt tgtcgttcag agcgatacca gctttagcat
     6541 cgaacacgga agtgcaaact tcgccgttga aatcggtaga tactacgtca tcttcggtgt
     6601 agcccagaac gcctttcatt tcgccttcag cagcagcttt aacggcagct ttgatctgct
     6661 cgtaagttgc agctttttcc agacgaacgg tcaggtcaac tacagatacg ttcggggtcg
     6721 gaacgcggaa cgccatacca gtcagtttgc cattcagttc tggcagtact ttacctacag
     6781 ctttagcagc accggtagag gacgggatga tgttctggga agcgccgcgg ccgccgcgcc
     6841 agtctttgtg agacgggcca tcaacggttt tctgagtagc ggtagtagcg tgaacggtgg
     6901 tcatcagacc ttcgatgatg ccgaagttat cgttgataac tttagccagc ggagccaggc
     6961 agttggtggt gcaggaagcg ttggaaacga tgtcctggcc agcatatttg tcgaagttag
     7021 cgcctttaac gaacatcgga gtgttgtctt tagacggacc agtcataacc actttcttcg
     7081 caccagcggt gatgtgttta cgagcagttt cgtcagtcag gaacagacca gttgcttcag
     7141 cgacaacgtc aacaccaact tcgtcccatt tcaggttagc cggatcacgt tcagcggtaa
     7201 cacggatttt tttaccgtta acgatcagat gaccgtcttt cacttcaacg gtaccgtcga
     7261 aacggccgtg agtggagtca tatttcagca tgtatgccat gtaatcagcg tctaacaggt
     7321 cgttgattgc aacgatctcg atgtcagaac gtttctgagc agcacggaaa acaatgcgac
     7381 cgatacggcc aaaaccgttg atacctactt tgatagtcat atattccacc agctatttgt
     7441 tagtgaataa aaggttgcct gtaaaattac aaaaacctta cgcagcgtca agcggaatcg
     7501 tgtcaatcat tgcgacaaat caatcctgtg cctaagcatt acgcgactga ctcgcctcac
     7561 tcttcctttg ggcttgagac cacatggggt cggcgccccg aattttaaag ggcaattacg
     7621 ataaaaatgt gattttcatc acgatttagg tgcagccatt tcgtcgagat caagtttagg
     7681 acaaaatttt gccagttggt gttaatgttt tgttagaatc agtcacgtaa tgtgagcacg
     7741 attaaagtga gatgtgagca aatggctaat aaaccttcgg cagaagaact gaaaaaaaat
     7801 ttgtccgaga tgcagtttta cgtgacgcag aatcatggga cagagccgcc atttacgggt
     7861 cgtttactgc ataacaagcg tgacggcgta tatcactgtt tgatctgcga tgccccgctg
     7921 tttcattccc aaacaaagta tgattccggc tgtggctggc ccagtttcta cgaaccggta
     7981 agtgaagaat ccattcgtta tatcaaagac ttgtcacatg gaatgcagcg catagaaatt
     8041 cgttgcggta actgtgatgc ccatctgggg catgtcttcc ccgacgggcc gcagccaacg
     8101 ggcgaacgtt attgtgttaa ctctgcctct ttacgcttta ccgatggcga aaacggcgaa
     8161 gaaatcaacg gttgaaaaaa cgattcagca acttattcca caggagcgga aaaattatga
     8221 atcttgatga cattatcaac agcatgatgc ctgaggtata ccagcgtttg tcgaccgccg
     8281 ttgaactggg gaaatggcct gatggcgttg cgttgaccga ggaacaaaaa gagaactgcc
     8341 tgcaactggt gatgctgtgg caagcccgcc ataatatcga agcacagcat atgacaattg
     8401 acacaaatgg tcagatggtc atgaagagca aacagcagtt aaaagaagat tttggtatct
     8461 cagcaaagcc tattgccatg tttaagtaag tggttcatcc cctttttgcc tcggctatga
     8521 aggggatagc caacaatgac cccacgactc atctcaatta tctcgccatg acctggctaa
     8581 gtcccagtcc gatctcttgc cgtcccgttc acacctgaat gacttctcct gaacttaagt
     8641 ctcagaccta tttggccggt aatccctctc gaatttgctc tttttgcgcc ccgccttcca
     8701 tcaacctgta acagacccag ccattttctg cgtaattgtg atctgagttg taaaacgcag
     8761 atcattatct gtgttttaca gatttaatgt tgacctcaaa aatcatgtgg gtaagtatga
     8821 tggccatcta agcaaaacta aacacagagg atgacgcaat gaaagcactg gctcggtttg
     8881 gcaaggcctt tggcggctac aagatgattg atgtcccaca acccatgtgt ggcccggaag
     8941 atgtagtgat tgaaattaaa gccgcggcaa tctgcggcgc agacatgaag cactacaatg
     9001 tcgatagcgg ttctgatgag tttaactcta tccgcggcca tgagttcgca ggttgtattg
     9061 cgcaggttgg tgaaaaagtc aaagactgga aagtggggca acgcgtcgta tcggataaca
     9121 gcggtcacgt ttgcggtgtt tgtccggcct gtgaacaagg tgattttctg tgttgtacag
     9181 aaaaagtaaa ccttggtctg gataataata cctggggcgg tggtttttcc aaatattgtc
     9241 tggttcctgg tgaaattctc aaaattcatc gtcatgcgtt gtgggaaatc cctgatggtg
     9301 ttgattatga ggacgcagcc gtacttgacc ctatctgtaa tgcctacaaa tccatcgcgc
     9361 agcaatcgaa attccttcct ggtcaggatg tggtcgtcat cggcactggc ccactcgggc
     9421 tgttctccgt acaaatggcg cgaattatgg gggcggtaaa tatcgtcgtc gttggtctgc
     9481 aagaagatgt ggcggtccgc ttcccggttg caaaagaact gggtgcgacg gcagtagtaa
     9541 atggttctac cgaagatgtg gtggcgcgct gccagcaaat ttgtggcaaa gacaatctgg
     9601 gactggtgat tgaatgctcc ggtgccaata tcgcactgaa acaagccatc gaaatgctcc
     9661 gcccgaacgg ggaagtggta cgcgttggaa tgggcttcaa acctcttgat ttctcgatta
     9721 atgacattac cgcctggaac aaaagcatca ttgggcatat ggcctatgac tccacctcat
     9781 ggcgtaacgc tatcaggctc ttagccagcg gcgctatcaa agtcaaaccg atgattacgc
     9841 atcgtatcgg cctgtcgcaa tggcgcgaag ggtttgatgc gatggtcgat aaaaccgcaa
     9901 tcaaagtgat catgacttac gactttgatg aataaccact ctcctgaagg aacgttatcg
     9961 catggaacag ataacaaaac cgcattgtgg tgcacggctg gatcgcttac cggattgccg
    10021 ctggcattca tcaatgtttg ccatcgtcgc gtttggcttg cttgtctgct ggagtaatgc
    10081 cgttggtggc ttgatcctcg cgcagctgaa agcgttgggc tggacagata attccaccac
    10141 agccacattc tcagcaatca cgaccgccgg aatgtttctc ggtgcgctgg tgggcggcat
    10201 cattggcgac aaaaccggtc gcagaaatgc gttcatcctc tatgaggcca ttcatattgc
    10261 ctcgatggtt gtcggtgctt tctcaccgaa tatggatttt cttattgcct gccgttttgt
    10321 gatgggagtt gggctgggag ctttactggt tacactgttt gctggtttca ccgaatatat
    10381 gcccggtaga aatcgcggaa cgtggtccag tcgggtttcc tttattggca actggtcata
    10441 tccgctctgt tcattgattg cgatgggact cacgccgctg attagtgcag agtggaactg
    10501 gcgggtacaa ctgcttatcc ctgcaatatt gtcgcttatc gctacggcgc tggcctggcg
    10561 ctactttcct gaatccccgc gctggctgga atcgcgcgga cggtatcaag aagccgagaa
    10621 agtcatgcgg agtatagaag aaggcgtcat acgccagacg ggtaaacctt tgccgcctgt
    10681 ggttattgct gatgacggta aagcgccaca agcggtgccg tattcagcct tactgacagg
    10741 agtattactg aaacgcgtga tattaggctc ttgtgtgctg attgccatga acgttgtgca
    10801 gtacacacta attaactggt tgccaacaat attcatgacc caggggatta atttaaaaga
    10861 ctcgattgtt ttaaatacca tgagtatgtt tggcgcgcca tttggtattt ttattgccat
    10921 gctggtgatg gataaaattc cgcgtaaaac aatgggtgtg gggctattaa tcctgattgc
    10981 ggtgctcgga tatatctatt cactgcaaac cagtatgttg ctcataacgt tgattggttt
    11041 cttcctgatt actttcgtct atatgtacgt ttgctatgcc tcggcagtgt atgtccctga
    11101 aatctggccg acagaggcca aactccgtgg ctccggtctg gcgaatgcgg tagggcgaat
    11161 caatggtatt gccgcacctt atgccgttgc agtgctgctc agtagttatg gcgtaacggg
    11221 agtctttatt cttctggggg ctgtttcaat tgttgtcgca attgctatcg ccaccattgg
    11281 aattgaaacc aaaggtgtct ccgttgaaag tttaagtatt gatgcagtag ccaataaata
    11341 atacgggtgg attcaaaatg aaaaattcaa aagcaatatt gcaggtgccg ggcacaatga
    11401 aaattatttc agcagaaata ccagtgccta aagaagatga agttttgatt aaagtagaat
    11461 atgtcggtat ttgtggttca gatgtacatg gttttgaatc aggcccgttt attccgccta
    11521 aagacccaaa tcaagaaatt ggcctgggtc atgaatgcgc cgggacggtt gtggctgtgg
    11581 gaagccgcgt gcgcaaattt aaaccggggg atcgggtaaa tatcgaacct ggcgttcctt
    11641 gcggtcactg tcgttactgt ctggaaggca aatataacat ctgcccggac gttgatttta
    11701 tggcgacaca acccaactac cgcggcgcat taacgcacta tctgtgtcat ccggagagct
    11761 ttacttacaa actgccagac aatatggaca cgatggaagg ggcgctggtg gagcctgccg
    11821 cagtcgggat gcatgccgcg atgctggcag atgttaaacc gggtaagaag ataattattc
    11881 tgggagcagg ttgtattggt ttgatgacgt tgcaagcgtg caaatgcctg ggcgcaacgg
    11941 aaattgccgt cgttgatgtg ctggaaaaac gtctgataat ggcggaacaa cttggtgcga
    12001 cagtggttat taacggcaca aaagaagaca ctatttcacg ctgtcagcaa tttaccgaag
    12061 acatgggcgc agatattgtt ttcgaaacag cgggttctgc ggtcaccatt aaacaggcac
    12121 cttatctggt aatgcgcggc ggtaaaatta tgattgttgg tactgtaccg ggcgattcgg
    12181 caatcaattt cctcaaaatc aatcgcgaag tcactatcca gacggtattc cgctatgcca
    12241 atcgttatcc ggtcacgatt gaagctattt cttcagggcg attcgatgtg aaatcgatgg
    12301 tgacgcatat ttacgattat cgggatgtac aacaggcatt tgaagagtca gttaacaaca
    12361 aacgcgacat tattaaaggc gttattaaaa ttagcgatta attaatacgg gagaacagaa
    12421 tatgctcgca gatatcagat attgggaaaa cgatgccaca aataaacatt atgcaattgc
    12481 ccattttaac gtatggaatg cagaaatgtt gatgggcgtt atcgacgcag ccgaagaagc
    12541 gaaatcaccg gttattattt cttttggtac aggttttgtc ggcaacacct catttgaaga
    12601 tttctctcac atgatggtat caatggcacg aaaagcaact gtgccggtaa taactcattg
    12661 ggatcatggt cggagtatgg agattattca taacgcctgg actcatggta tgaattcatt
    12721 aatgcgtgat gcttctgcat ttgatttcga agaaaatatt cgtttaacca aagaggctgt
    12781 cgatttcttc catccgctgg gtattccggt agaggcggaa ttagggcatg tcggtaatga
    12841 aaccgtttat gaggaagcgc tggcgggcta tcactatacc gatcctgacc aggcggctga
    12901 atttgttgaa cgtacgggct gtgactcatt ggctgtcgcc atcggcaacc agcatggggt
    12961 ttatacgtca gagccacaat tgaactttga ggtcgtcaaa cgcgtacgcg atgccgtttc
    13021 tgttccgctg gttttgcacg gtgcatcggg gatcagtgat gccgacatta aaactgcaat
    13081 ttcgttgggt atcgcgaaaa tcaacattca tacggagctc tgtcaggccg caatggtcgc
    13141 agtgaaagaa aaccaggatc agcctttcct gcatttagaa cgtgaagtac gtaaagctgt
    13201 aaaagaacgg gcattggaaa aaattaaact gttcggttct gatggcaaag cggaataatg
    13261 atatggataa tctcgacgtt atttgtatag gtgccgctat tgttgatatt ccattgcaac
    13321 cggtcagtaa aaatatcttt gatgtggatt cttatcctct tgaaagaatc gcaatgacca
    13381 ccgggggtga tgcaataaat gaagcaacaa ttatttctcg tctgggccat cgtacagcat
    13441 taatgagccg tattggtaaa gatgccgcag gacagtttat tctcgaccat tgccgcaaag
    13501 agaatattga tattcaaagc ctgaagcagg acgtcagcat agatacctct attaacgttg
    13561 gactggtgac ggaggatggc gagcggacgt ttgtcacaaa ccgtaacggc agtctgtgga
    13621 aattgaatat tgacgatgtt gatttttcgc ggttttctca ggcgaaatta ttatctctgg
    13681 ccagtatttt caacagtcct ctattggatg gtaaagcgct aacagaaatt tttacgcaag
    13741 ctaaaacccg gcagatgatt atctgtgccg atatgatcaa accgcggttg aatgaaacgc
    13801 tggatgatat ttgcgaagca ttgagttatg tcgattatct gtttcctaat tataccgagg
    13861 caaaattact caccgggaaa gagacattgg atgaaattgc tgactgcttt cttgcgtgcg
    13921 gcgtaaaaac ggtggtgatt aaaacgggta aagacggctg ctttatcaag cgtggtgaca
    13981 tgacgatgaa ggtgccggcg gtcgcaggaa taaccgccat cgacaccatt ggcgcgggcg
    14041 ataactttgc ttcaggtttt attgcggcac tgttagaagg caaaaatctg cgtgaatgcg
    14101 cacgctttgc caatgcaacg gcggctatct cggttctaag cgtcggtgcc accaccggcg
    14161 taaaaaacag aaagctggtc gaacaattgc tggaagaata cgaaggataa tgaaggcaaa
    14221 tgaaaaagat acctttaggc acaacggata ttacgctttc gcgaatgggg ttggggacat
    14281 gggccattgg cggcggtcct gcatggaatg gcgatctcga tcggcaaata tgtattgata
    14341 cgattcttga agcccatcgt tgcggcatta atctgattga tactgcgcca ggatataact
    14401 ttggcaatag tgaagttatc gtcggtcagg cgttaaaaaa actgccccgt gaacaggttg
    14461 tagtagaaac caaatgcggc attgtctggg aacgaaaagg aagtttattc aacaaagttg
    14521 gcgatcggca gttgtataaa aacctttccc cggaatctat ccgcgaagag gtagaagcca
    14581 gcttgcaacg tctgggtatt gattacatcg atatctacat gacgcactgg cagtcggtgc
    14641 cgccattttt tacgccgatc gctgaaactg tcgcagtgct taatgagttg aaggctgaag
    14701 ggaaaattcg cgctataggc gctgctaacg tcgatgctga ccatatccgc gagtatctgc
    14761 aatatggtga actggatatt attcaggcga aatacagtat cctcgaccgg gcaatggaaa
    14821 acgaactgct gccgctatgt cgtgataatg gcattgtggt tcaggtttat tccccgctag
    14881 agcagggatt gttgaccggc accatcactc gtgattacgt tccgggcggc gctcgggcaa
    14941 ataaagtctg gttccagcgt gaaaacatgc tgaaagtgat tgatatgctt gaacagtggc
    15001 agccactttg tgctcgttat cagtgcacaa ttcccactct ggcactggcg tggatattaa
    15061 aacagagtga tttaatctcc attcttagtg gggctactgc accggaacag gtacgcgaaa
    15121 atgtcgcggc actgattatc aacttatcgg atgcagacgc aacattgatg agggaaatgg
    15181 cagaggccct ggagcgttaa atactcaaca cgattcacct ctgtttctgt gaaaactgtg
    15241 atgaaaatct gtgattcaaa caggttgttt tgaaagtaaa catcggttat gcgataatcg
    15301 cgctaatgtg atgtaaaaag atacaggggt gtaatcgtgg cggcaaaaga caggattcag
    15361 gcaattaagc aaatggttgc caacgataaa aaagtgacag tctcaaattt gagtgggatt
    15421 tttcaggtaa ccgaagaaac cattcgccgc gatcttgaga agctggaaga tgaaggcttt
    15481 ttgaccagaa cctatggtgg tgctgtttta aatacagcga tgttgacgga gaatatccat
    15541 ttttataagc gcgcttcatc gttttatgaa gagaagcagc ttattgcacg caaggcacta
    15601 ccctttatcg acaataaaac caccatggca gccgattcca gtagtaccgt aatggaattg
    15661 ctcaaattgt tacaggaccg tagtgacctg acgttgctaa ccaactcggc ggaagcgatt
    15721 catgttctgg ctcagtcaga aattaaagtc gtttcaacgg gtggggaact aaacaaaaat
    15781 acactttcac tgcaaggaag aattactaaa gagatcatca gccgctatca tgtcgatatc
    15841 atggtaatga gttgtaaagg tcttgatatt aacagcggcg cgctggactc taatgaagcg
    15901 gaagctgaaa tcaaaaagac aatgatccgt caggcgacag aagttgcgtt attggttgat
    15961 cactctaagt ttgatcgcaa agcttttgtc cagttagctg attttagtca tattaattac
    16021 ataataactg ataaatcacc gggtgcagag tggatagcat tttgcaaaga caataatatc
    16081 cagctggtgt ggtaatttaa gcacacgttg tcaatattat ttgttcctgc gccttgaaat
    16141 aaaatgatga taaagatgcg gcaagtgaat ttgccgcaat tgctaccttt ttagcaaatc
    16201 cgaggcacct aatggaacaa tatgatcaaa ttggcgcaag actggaccgc ttgcctttgg
    16261 cccggtttca ttatcgtata tttggtataa taagctttag tctgttatta acggggtttt
    16321 tgagttactc aggtaatgtc gtcttagcaa agctggtaag caatggatgg tcaaataatt
    16381 tcctcaatgc cgcctttacc tcggcattaa tgtttggtta tttcatcggc tcacttactg
    16441 gtgggtttat tggtgactac tttgggcggc gcagggcgtt tcgcataaat cttctcatcg
    16501 tcggtattgc tgcaacaggg gccgcttttg tccctgatat gtactggctc attttctttc
    16561 gcttcctgat gggaacagga atgggggcgc tgattatggt tggctatgcc tcatttacgg
    16621 agtttatccc cgcgacggtg cgtggaaaat ggtccgcgcg gctctcattt gttggtaact
    16681 ggtcgcccat gctgtctgcg gcgataggcg tggtggttat cgcttttttt agttggcgaa
    16741 taatgtttct tctgggtggt attggcatac tattagcctg gtttctctca ggtaaatact
    16801 ttatcgagtc gccacgatgg ctggcaggga aagggcaaat cgccggtgca gaaagccaac
    16861 ttcgtgaagt agagcagcaa attgaaaggg agaagagtat tcgtttaccc cagcttactt
    16921 tgaaccagag caacagcaag gttaaggtaa tcaagggtac tttctggctc ctgtttaaag
    16981 gggaaatgtt acgacgtaca ttagttgcga taactgtttt aattgcaatg aacatttcgc
    17041 tttataccat taccgtatgg ataccgacca tatttgttaa ctccgggatt gatgtcgata
    17101 aatcaatatt aatgaccgct gttattatga ttggtgctcc ggtaggaata tttattgcgg
    17161 cattaattat tgatcatttt cctcgtcgat tatttggctc cgccttactt attattattg
    17221 ccgtgttagg ctatatctat tcaattcaga ctacagagtg ggcgatttta atctatggtc
    17281 tggtgatgat cttcttttta tacatgtatg tttgcttcgc gtcagcggtt tatatcccgg
    17341 agctttggcc aacgcattta cgcctgcgcg gttcgggttt cgttaatgcc gtcggacgga
    17401 tcgtcgcagt tttcacgccc tatggcgttg cggcattatt aacacattat gggtcgatca
    17461 cggtgtttat ggtgcttggt gtcatgttag tgctctgtgc gctggttctc tccatttttg
    17521 gcatcgaaac gcggaaggtg tcgctggaag agatttctga ggtgaattaa atctttcgtt
    17581 gatggcatca attgccagat gtaccactga cgtgttttat ttggcctaca atgccagtac
    17641 agaattgtag gccagcgtaa aattacccct gtgtctcttc ccagtctgcc agcgtatata
    17701 gcgttgcccc agctgctgac atctccataa acgcgtgtgc actgtcctgg ggctggatat
    17761 tcacgccacg acaaccatcg gtaatcacgt tcaccttata acctaactgt aacgcgtcca
    17821 gcacggtaaa cttcacgcaa tagtcagtag ccaggcccat aacgatcaat tcatcgattt
    17881 catgatcgcg taaccagtca tcgagagagg ttttctgccg acggccgtta tcaaaaaagg
    17941 cactgtaact gtcaactaaa ggattttcgc ctttatggaa caccgctgcg atcgcttttt
    18001 ggttcagtaa cggatgtaat tgtgcgcctt cactgttctg cacacagtga tctggccaga
    18061 aggtttgtgg caaaccgtcg agttggcctg gcgtataagg ctctacaccg tgctgactgg
    18121 caaaactgcc gtgattcgcc gggtgccagt cctgactggc gataaccgct tctccgcgcg
    18181 actggcacca gtcaataagg cggttagcaa cctccaccgt actgtcacct tccggtacgg
    18241 cgagcgcgcc accagcacag aaatcatttt gtaaatcgac cagtaacagg gcgcgatggg
    18301 gcattgcagt ctccttaatc atccggcgtc agttcgccgc gcaggttttg gctcatggcc
    18361 ttgcgaatgg tttcagtatc cagttcctgg ctcagcaggt aatgcagttt ggttagcgtg
    18421 gcttctacag tcatatctgc accgccaata acgccggcat gggcgagggc gttaccggtg
    18481 gcataaccac ccatattcac tttaccggac atacattgtg tcaggttgac caccacaata
    18541 ccgcgatcgc tggcttcttg taattcctgc aggaaggctt tgttttgtgg cgcattaccc
    18601 acgccatagg agcgcagaat caatgctttc accggttggc gcagaaaatt gcgcacgacg
    18661 tcagcagaaa tccctggata aatcgtcact acgccaattg gttgtggcgt gattggatga
    18721 acgattaatt ccccttcacc gtgcggggcg ggtggcgtat tcaaacgacg aatatggata
    18781 ccagcttcca gtaacggagg aaggtttgga gaggcaaacg catcaaaacc atcggcatgg
    18841 gctttggtag tgcggttgcc gcgatacaat cggttattga aaaagagcgt tacttcgttg
    18901 atcggataat tcgccgcaac gtacaacgca ttcagcagat taatttgtcc gtcagagcgt
    18961 aactcagcca gcgggatttg tgaccctgtc acaatgaccg gtttaccgag attctcgagc
    19021 atgaacgaca gcgcagaggc ggtatacgcc atcgtgtcgg taccgtgtag aatgacaaaa
    19081 ccatcatagt cgtcatagtg cgctttaata tcttcagcaa tatgctgcca gtcttccggc
    19141 gtcatatcag aagaatccat caacggcgta tattcatgaa tggtgaaatc tggcatctcc
    19201 gggcgatgga attccggcat cagcgccagt tggcgttgta gatgacctga caccggaata
    19261 taaccctgct cggaacgctg catcccgatg gtcccgcccg tgtaggcgac gtaaattgat
    19321 ttcttttgca tgatattgat atgaatgata tcgaaagagc aaaagtatat acgtgaggca
    19381 ggaggagcgc acctgatact atgcggataa ggaaatatgg ggcgagacag cgggaagagg
    19441 attcgtcgag gtaaaaaata atgtggccga cgatctgtcg gccactcagt acaagactta
    19501 acgcacgttg gcgcaggtca ggcaaaacgc ataacggttt tgtgggtcgt taaacgcggc
    19561 cagcttgtcg ctttcacttt taacggtaga ggctaccgag gcaagcggtg caggtaacat
    19621 ggcctggaac gcatctggca acattgcccg gacagaacca gacatgttgt ccatcacttt
    19681 gtcgaagaag gtcggttcat caacgtagta ttccagatgc cactgtttca cttttgcgag
    19741 ctctgctgct ttggcaaccg catcatcgaa atccccgaga ctatcgacca gcccgttagc
    19801 ttttgcatcc tgaccggtcc agacgtggcc ctgggcgatt ttatcaattt gctccggcgt
    19861 cgaatgacgc gcatcagcaa ccagcgtgat aaagcgttta tagccattct caatgcttaa
    19921 ttgcatcatc tgctgcgctt ccggcggcag tgccctggtg atagaaacat ccgccagcgg
    19981 tgaagttgag acaccatcag tatgaacacc aatcgaatcc agactatttt ctacggtggt
    20041 gatcacgccg aagataccga tagaaccggt cagggtgctg gggttagcca caatgtaatt
    20101 agctggcgtg gaaatccagt aaccaccaga tgccgccatg ccgcccatcg atacaaccac
    20161 aggcttaccc gctgcccggg ctgctgccag ttcagcgcga atcacttcag acgcggtaac
    20221 gctgccgcct gggctattaa cacgcaggac aatcgctttc actttcgggt caaggcgagc
    20281 gtcgcggatt tgtgccgcag tggtatcacc gccaacattc ccctgagttt cctcgccatc
    20341 cataattgcg ccattagcga agacgacacc gatgctgtca ccggtatctg ccggcgtttt
    20401 caatgcgtaa tcgtaataac tgatggcgcg ataattttta tcagtcttac tccagccgaa
    20461 ttctttggtc agtgcttttt cgatttccgc actcgatgcc agtgcatcga ccagcttgtt
    20521 ttccagtgca tatttcgcgg tatcgccacc ggttttggtt aaaccctcaa gcaacccttg
    20581 cgcgccaggg aatacctgct cagcagggat ctgccggtta gcggcaacag tattcagata
    20641 gttttgccac agctcaccaa tccagcggct gtcagcttcg cgggctgccg gtgacatatc
    20701 atcacgaata aacggttcaa cggcagattt atacgtaccc acgcggaaca catgggtgga
    20761 aactttcagc ttatccagca acgatttgta gtacagaccg ttggtggcaa agccgtgcag
    20821 atcaaccacg ccttgcggag acagccaaat tttattggcg aaactggcga gataatattg
    20881 cccctggctg tagttctcgc caacggcata aaccggtttc ccgctgtcac gaaactcttt
    20941 cagagctttg ccgatgtact gcatagacgg ttggtcgccg cctgcgaagt ttttcagatc
    21001 catcacaata ccggtgatat tccggtcgtc cttcgcctgg cgaatggtgt tgacgatatc
    21061 aaacagtgag ttttcctgca gacgatcgga actggcacca agcagctggc ggcttaattt
    21121 actaaaccgc tgagaactgt cgggtttatc gacgatcaca ccagaaatgt ccagcagcag
    21181 tgcgccacga ctggccgttt ctttcgaatc accaccactg acctgcatcc agatccccac
    21241 accaactagt acgagaaaaa taaagaacag gttaagtacc atttcacgga cgaaattcag
    21301 cagacgccac gtccatttaa aaaatccggc aataaatcgc caaagggttc gcatgtattc
    21361 tcccaactta aggtcacacc tgtcacaggc gcaatagatg tggttatcgt aatgacccaa
    21421 ccggtacttg tcagcaggaa ttgcggactg tgctgtaaca aaatccactc tcgtgttaat
    21481 tttgtgaata aatatcacga caggagttaa tcaaatggat gcactcgaac tattgatcaa
    21541 tcgccgtagc gcctcccgcc tggctgaacc cgcaccaacg ggtgaacaac tgcaaaacat
    21601 cctgcgtgcg ggtatgcgcg cgccggacca taagtccatg caaccgtggc atttttttgt
    21661 gattgaaggg gaagggcgcg agcgtttcag cgccgtactg gagcaggggg cgattgctgc
    21721 cggtagtgat gacaaagcca tcgacaaagc gcgtaatgcg ccgttccgcg caccgctcat
    21781 catcacggtg gtggcgaaat gcgaagagaa tcataaagtc ccgcgctggg aacaggaaat
    21841 gtctgccgga tgcgcggtca tggcgatgca aatggcagcc gttgcccagg gttttggcgg
    21901 catctggcgc agtggcgcat taactgaaag cccggtagtg cgtgaagcat tcggttgccg
    21961 tgagcaggat aaaattgtcg gttttctcta cctcggtacg ccgcagctga aagcatctac
    22021 gtcgattaac gtcccggacc cgacgccgtt tgtaacttat ttctgataat tgtcgcgaaa
    22081 ctgtctggat tgtgagcaaa ggcgcaggaa ttcagacact ctcacttatc acttcacgga
    22141 atgagggtta ccatacccgg attgcattga caggagatgt ccatgagcga gaactcgatt
    22201 cgtttgaccc aatacagcca cggagctggt tgcggctgta aaatttcccc aaaagtgttg
    22261 gaaaccatcc tgcacagtga gcaggcgaag tttgttgatc cgaatttgct tgtgggtaat
    22321 gaaacccgcg acgatgcggc ggtgtacgat ctgggcaatg gcaccagcgt tatcagtacc
    22381 accgacttct ttatgccgat cgttgataat cctttcgatt ttggccgcat tgcggcgact
    22441 aacgccatca gcgatatctt cgcgatgggg ggcaaaccga ttatggcgat tgcgatcctc
    22501 ggctggccga ttaacaaact ttccccagaa attgcccgcg aagtgaccga aggtggacgc
    22561 tatgcatgtc gtcaggcggg tattgcgctg gctggcggtc actccatcga tgcgccggag
    22621 ccgatttttg gtctggcggt aacggggatc gtaccgaccg agcgggtgaa gaaaaacagt
    22681 accgcacaag ccggatgcaa actgttcctg acgaaaccgc tggggatcgg cgttcttacc
    22741 acggctgaga aaaaatcact gttgaaacca gaacatcagg gactggcgac ggaagtgatg
    22801 tgccggatga acatcgcagg cgcgtccttt gccaacatcg aaggcgtaaa agcgatgacc
    22861 gacgttacgg gctttggtct gctgggccac ttgagcgaaa tgtgtcaggg ggctggtgtg
    22921 caggcacgcg tcgactatga agcgatcccg aaactccccg gtgttgaaga gtacattaag
    22981 ttgggcgcag tacctggcgg cactgaacgt aactttgcca gctacggtca tctgatgggt
    23041 gaaatgccgc gtgaagtgcg cgatctgctg tgcgatccgc aaacttctgg cggtttgctg
    23101 ctggcggtca tgccggaagc agaaaatgag gtcaaagcta cagccgccga gtttggcatt
    23161 gaactgacgg caattggcga actggtgcca gcgcgcggcg gtcgtgccat ggttgagatt
    23221 cgttaattca atgcggttgt ttattgccga aaaaccgagt ctggcgcgcg ccattgctga
    23281 tgtcctgccc aaaccgcacc ggaaaggcga tggctttatc gagtgcggta atggtcaggt
    23341 ggtgacctgg tgtatcggtc acctgcttga gcaggcgcag ccagacgcct acgacagccg
    23401 ctatgcgcgc tggaatcttg cggatttgcc gattgtcccg gaaaagtggc aattacagcc
    23461 ccgaccctcc gtgaccaaac aacttaacgt catcaaacgc ttcctgcatg aagccagcga
    23521 aatcgttcac gccggggacc cggatcgtga agggcaattg ctggtggatg aagtgctgga
    23581 ctatctgcaa ctggcaccgg aaaagcgcca gcaggtgcag cgttgcttga taaacgacct
    23641 gaacccgcag gcggttgagc gggcgatcga ccgtcttcgt tccaacagtg agtttgtacc
    23701 gctgtgcgtt tctgcgctgg cgcgagcgcg tgccgactgg ctgtacggca tcaatatgac
    23761 ccgtgcttac accattctcg gtcgcaatgc tggttatcag ggcgtgcttt ccgtggggcg
    23821 cgtgcagaca cccgtgctcg ggctggtggt gcgccgcgat gaagagattg aaaacttcgt
    23881 ggcgaaagac ttctttgaag tcaaagcgca tatcgtgaca cctgccgatg agcggtttac
    23941 cgctatctgg caaccgagcg aagcgtgtga accgtaccag gatgaagaag ggcgcttgtt
    24001 acatcgtcca ctggcggagc atgtggttaa ccgcattagt ggtcaaccgg ctattgtcac
    24061 cagctataac gataaacggg aatcagaatc cgcgccgctg ccgttttcgc tttcggcgtt
    24121 gcagattgaa gcggcaaaac gctttggtct gagcgcgcag aacgtgcttg atatctgcca
    24181 gaaactgtac gaaacgcaca agctaatcac ttatccgcgt tctgattgtc gctatttgcc
    24241 agaagaacat tttgccggac gccacgcggt gatgaatgcc atcagtgttc atgcaccgga
    24301 tctgttgccg cagccagtgg tagatccaga tatacgcaac cgctgttggg atgacaaaaa
    24361 ggtcgatgcg caccacgcca tcattccgac cgcacggagt tctgcgatca acctgacgga
    24421 gaacgaagcg aaggtctata acctgattgc ccgtcagtat ctgatgcaat tctgcccgga
    24481 tgcggtgttc cgcaagtgtg ttatcgaact ggacattgcc aaaggcaaat ttgtcgctaa
    24541 agcgcgtttt cttgctgaag caggctggcg cacgctgtta ggcagcaaag agcgcgatga
    24601 agaaaacgac ggcacgccac tgcctgtggt ggcgaaaggc gatgagttgc tgtgtgaaaa
    24661 aggtgaagtg gtagagcggc aaacccagcc gccgcgccat tttaccgatg caacactgct
    24721 ttcggcgatg accgggatcg cgcgctttgt gcaggacaaa gatctgaaaa agatccttcg
    24781 tgcgaccgat ggtctgggga cagaagcaac gcgtgccggg attattgaac tgttgttcaa
    24841 gcgtggtttc ctcaccaaaa aagggcgcta tatccactcc accgacgccg gaaaagcgtt
    24901 attccattcg ctgccagaaa tggctacgcg accggacatg accgcgcact gggaatcggt
    24961 gctgacgcaa atcagcgaaa agcagtgtcg ctatcaggac tttatgcagc cgctggtggg
    25021 gacgctttac caactgattg atcaggcgcg tagcactccg gtcaggcagt tcagaggaat
    25081 tgtggcaccg ggtggggagt cagttaaaaa agcacccaat aaacgagtga aaaaagcggc
    25141 aaagccccaa aaagataata cggattctgc taattaattc ggtttccac
//
LOCUS       DAJOSY010000063        25138 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJOSY010000063 DAJOSY010000000
VERSION     DAJOSY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.063"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            145..2589
                     /locus_tag="OHN27_002693"
     CDS             145..2589
                     /locus_tag="OHN27_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCQ2287102.1"
                     /translation="MPTPTIIKTLNTHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKRFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFILGASTPVNAALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQIVLLNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDGWQPLLRTDKWLACYPSLCYQRPGFSDIEKKTTDN
                     IAHYFNKLPVATKPSTLPIADTIGFFMETSFHYTLYRPIITALQAQGQSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(2707..3102)
                     /locus_tag="OHN27_002694"
     CDS             complement(2707..3102)
                     /locus_tag="OHN27_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HCQ2287103.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(3105..3539)
                     /gene="fliJ"
                     /locus_tag="OHN27_002695"
     CDS             complement(3105..3539)
                     /gene="fliJ"
                     /locus_tag="OHN27_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HCQ2287104.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTIELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAASVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(3542..4879)
                     /gene="fliI"
                     /locus_tag="OHN27_002696"
     CDS             complement(3542..4879)
                     /gene="fliI"
                     /locus_tag="OHN27_002696"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HCQ2287105.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(4872..5582)
                     /locus_tag="OHN27_002697"
     CDS             complement(4872..5582)
                     /locus_tag="OHN27_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein H"
                     /protein_id="HCQ2287106.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTASISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     ETITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(5586..6596)
                     /locus_tag="OHN27_002698"
     CDS             complement(5586..6596)
                     /locus_tag="OHN27_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCQ2287107.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQVQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(6574..8220)
                     /gene="fliF"
                     /locus_tag="OHN27_002699"
     CDS             complement(6574..8220)
                     /gene="fliF"
                     /locus_tag="OHN27_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCQ2287108.1"
                     /translation="MNAQIKKLTQAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLSYRIDPQSGQILVPEDELSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDVPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTRASVANVLDSLVGTGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTTQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQVAPVEPVIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPLMKRLTAVRTPVIAPEPEAVSEPWIATPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(8225..8566)
                     /locus_tag="OHN27_002700"
     CDS             complement(8225..8566)
                     /locus_tag="OHN27_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCQ2287109.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     NRIMSGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(8581..9567)
                     /locus_tag="OHN27_002701"
     CDS             complement(8581..9567)
                     /locus_tag="OHN27_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HCQ2287110.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENDNAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDVLGLPETDVADRSELQWPVQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKQGIEVFS"
     gene            9955..10806
                     /locus_tag="OHN27_002702"
     CDS             9955..10806
                     /locus_tag="OHN27_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HCQ2287111.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAEMMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESSFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            10799..11170
                     /locus_tag="OHN27_002703"
     CDS             10799..11170
                     /locus_tag="OHN27_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HCQ2287112.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            11167..11919
                     /gene="fliP"
                     /locus_tag="OHN27_002704"
     CDS             11167..11919
                     /gene="fliP"
                     /locus_tag="OHN27_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCQ2287113.1"
                     /translation="MKRRTQLTLGLGLLALAPLALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GNAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            11922..12194
                     /gene="fliQ"
                     /locus_tag="OHN27_002705"
     CDS             11922..12194
                     /gene="fliQ"
                     /locus_tag="OHN27_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliQ"
                     /protein_id="HCQ2287114.1"
                     /translation="MLTVDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            12196..12978
                     /gene="fliR"
                     /locus_tag="OHN27_002706"
     CDS             12196..12978
                     /gene="fliR"
                     /locus_tag="OHN27_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCQ2287115.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPIPHDLLSLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            12968..14107
                     /gene="flhB"
                     /locus_tag="OHN27_002707"
     CDS             12968..14107
                     /gene="flhB"
                     /locus_tag="OHN27_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCQ2287116.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSSEHLAETGKMTVKSVVVLVMLWI
                     SLRNNFAAFLGLQALPFKLAMNEGLSLYASVMRNFVILFIFFALLDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDQSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRDISIPKEVLKLDGENN"
     gene            14091..16184
                     /gene="flhA"
                     /locus_tag="OHN27_002708"
     CDS             14091..16184
                     /gene="flhA"
                     /locus_tag="OHN27_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCQ2287117.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGAGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALM"
     gene            complement(16408..16905)
                     /gene="rayT"
                     /locus_tag="OHN27_002709"
     CDS             complement(16408..16905)
                     /gene="rayT"
                     /locus_tag="OHN27_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCQ2287118.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRHRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHT
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(17081..17830)
                     /locus_tag="OHN27_002710"
     CDS             complement(17081..17830)
                     /locus_tag="OHN27_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCQ2287119.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRISRLAEPFWQDHFLGARRILTEETIL"
     gene            18131..18871
                     /locus_tag="OHN27_002711"
     CDS             18131..18871
                     /locus_tag="OHN27_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCQ2287120.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(18842..19609)
                     /locus_tag="OHN27_002712"
     CDS             complement(18842..19609)
                     /locus_tag="OHN27_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCQ2287121.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(19815..20393)
                     /gene="lpcA"
                     /locus_tag="OHN27_002713"
     CDS             complement(19815..20393)
                     /gene="lpcA"
                     /locus_tag="OHN27_002713"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCQ2287122.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            20633..23077
                     /gene="fadE"
                     /locus_tag="OHN27_002714"
     CDS             20633..23077
                     /gene="fadE"
                     /locus_tag="OHN27_002714"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCQ2287123.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALVKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(23120..23566)
                     /gene="ivy"
                     /locus_tag="OHN27_002715"
     CDS             complement(23120..23566)
                     /gene="ivy"
                     /locus_tag="OHN27_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCQ2287124.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            23747..24517
                     /gene="yafV"
                     /locus_tag="OHN27_002716"
     CDS             23747..24517
                     /gene="yafV"
                     /locus_tag="OHN27_002716"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCQ2287125.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(24559..>25138)
                     /locus_tag="OHN27_002717"
     CDS             complement(24559..>25138)
                     /locus_tag="OHN27_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCQ2287126.1"
                     /translation="QGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSH
                     GREEIRLHIVCDVPDELIDFTFEWKGLKKLCMAVSFRSIIAEQKKEPEMTVRYYISSA
                     DLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTN
                     DKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         5475 a         6899 c         6830 g         5934 t
ORIGIN      
        1 cctctcccca aaggggcgag gggaccgatc gagcacagtg ttaaaatcgg cgctgatttt
       61 ctccctctcc ctgtgggaga gggttggggt gagggcatca gcgcgcacat tcatcacatt
      121 cacccattaa ctcacccaaa caccatgcca acaccaacca tcatcaaaac actcaacacc
      181 caccgcgaaa aaccgtttgt cagcgtcgtc acgccaacct ggaaccgcgg cgcattcctg
      241 ccgtacctgc tctatatgta ccgctatcag gattacccgg cagaccgccg cgaactcatc
      301 atcctcgacg attccccaca aagccatcaa cacatcatcg accgcctgac caatggcacg
      361 ccggaagcct ttaacatccg ctacatccat cacccggaaa aactgccgct gggcaaaaaa
      421 cgcaacatgc tcaacgaact ggcgcgcggc gaatacatcc tctgcatgga cgatgacgac
      481 tactacccgg cagataaaat ctcttacacc atcgccatga tgcaaaaaca ccgggcgctc
      541 atctccggct ccgatcagat ccccatctgg tacagccaca tcaaccgcat ctttcaaagc
      601 cgcagctttg gcccgcataa catcctcaac ggcacctttt gctatcaccg taactacctg
      661 aaaaagcacc gttacgacga tgattgcaat ttaggcgaag agaaacgttt caccgacaat
      721 ttcagcgtaa acccgctgca actgcccggc gagcgcacga ttctgtgtat ttcgcatagt
      781 cacaacacct ttgataaaga ttttattctc ggggccagca cgccagtaaa cgcggcgctt
      841 acggatatcg tccgcgatcc gctgctgcgc aatgcttatc tgagtctgca taacgccact
      901 catcaccagg cgatcaacca tcaggcgatc gatcagatcg ttctgctgaa tctggataaa
      961 cgcccggatc gcttacaaca gatccgtgaa gaactggcat tgctgcatat tccacctgag
     1021 aagatcaccc gccttgccgc cagtgaagat gaaaacggtc aacggggccg ccgtcagtcg
     1081 cacttacagg cgctgcgcct ggcgcaacag cacggctggc aaaactacct gctgctggaa
     1141 gatgacgcgg ttattctcaa gcaggagaaa catattcagg tactgaacac gctgctggcg
     1201 agcctggcga aaattccctg gcaggtgatg atcctcggtg gcgaaatttc gcagggaacg
     1261 atgctaaaaa gtttgccggg gctggtgcac gcgcgggact gccgcaaagt gtgcgcgtat
     1321 ctggtgaaca gccgctacta cccgcaactg gcgcagcaga tgagcaacga tgagcactcg
     1381 ctggaagacg gctggcagcc gctgctacgc accgacaaat ggctggcctg ttacccgagc
     1441 ctttgttacc agcgaccggg ttttagtgat atcgagaaaa aaacgactga taatattgct
     1501 cactatttca ataagttacc cgtagccacc aagccatcta cattacccat cgccgatacc
     1561 atcggctttt ttatggaaac ctcattccac tacacgctgt accgcccaat catcaccgct
     1621 ctacaggcgc aggggcagag ctgcacacta gtgatcaatg accgcgtgtt taaaccgttt
     1681 cttgatgaga tgctggaaac actgaaaaac atcgacgatc cgcagcttaa aggtatgcgt
     1741 ctgtcagaaa tgcagacaca tggtcagcgg gtgaaatgcc tggtcagccc ctaccacacg
     1801 cctgcgctca atggcctggc ggcggtgaac atccgcgcta tgtacggctt agccaaagag
     1861 acgtggaacc acgccgactg gaatcgtttt taccaaagca ttttgtgtta tagccactac
     1921 agtcagcagg cgctggcgca tttcggcagc gcaaaagtgg tcggcaaccc gcgcttcgac
     1981 gcctggcata acggcacgtt cgatcgcgcg ttgccggaaa acatccaatc agattaccgt
     2041 aaacctacgg tgctctatgc cccaacgttt ggcgcattaa gctccctgcc ccactgggct
     2101 gaaaaattgg gccgcttgag cggtgatgtg aacctgattt gcaaactgca ccatggcacc
     2161 tgctcacgcc cggaagaagc agcatcgctg gccctggtgc gcaggcattt aaaacagcgc
     2221 accgactccg cccgccacac gctggcgctg ctggcaaaag ccgattacgt gctgaccgat
     2281 aacagcgggt ttattttcga tgctatccac gttgataagc gggtgatcct gctcgacttt
     2341 ccgggcatga atgatttgct cgacggtgag aaaagttatt ccacggcaga gagcgccgac
     2401 cagcgaatcc gcgaaatatt gccggttgct catgatgttg cagaactgcg ttatttgttg
     2461 tcagaggcgt ttgactgggg gtcggtgcag gcgcggctta cggagattcg ccatcactat
     2521 tgcgatgcgt ttatggatgg caaggcggga gaacgggcgg cgattgtgat tgtggaggcg
     2581 ctggggtaaa ccgcgcaact gcctgatgcg cttcgcttat caggcctacg gatcgatcac
     2641 gtcctgtagg ccggataagg cgtttacgcc gcatccggca agaaaataca tctctgtttt
     2701 agtactttat aaaagccctc taatcacctc aataatcccc gtcgtcgaca ccgacggcgt
     2761 gcgggggaaa tagaccacct cacaaatata cgcaaagctg tcaaacttcc ctgcccagtc
     2821 atcccccatc actaaaatat ccgcactgta cccgcgcaaa tactccgctt tctgctctag
     2881 cgattcttcc agaaatacgc catccacgca cgccagcccg gcgatgatcg ccatgcggtc
     2941 atcctgatgg taaaccggtg ccctgccctt tttggcgatg ttcagggcgt cggacgatac
     3001 gccgaccagc agccgctcgc ccaatgcgcg ggcgcgttgt aaaaggcgca gatgaccgac
     3061 atgaaacaca tcgaaggtgc cgaaggtgat gaccgttttc atggctactc cgctaatttg
     3121 tgccgcagcc agcactgcac ggcgatgtcg tccaccgctt tttgcgcttt cgccgtggcg
     3181 ctttcttgtt gctcgcggcg tttgtcgtcc agcgtcaggg cgactacttt ctcttcgcac
     3241 gccgccgccg tgaggttttt ctgcattcgc gtggctttga tattggccag cgttttttcc
     3301 tgctcctgcc aggcgattac cttgcgcagg gtgcctttgt agccgctgac gtttttcagc
     3361 gactccaccg atgccgccgc gcccgcagac gttttctcca ccaggtagcc taacgccttg
     3421 atattgttgt catagcccgc gcaaatctgt ttttgctttg ccagctcgat ggttttgtct
     3481 ttgaccgatt tatcccgcag gcgctgcaac tgcgccaggg tgtcgatgat ttgtcgcata
     3541 gttactgttc tgcctttttg acaccaggaa agagaatttg caggcggctt tgcaccagtt
     3601 cgagggaggc gggttcgcgc atctcctggc gcaaaaatcg ctcaatggcc ggaaacattt
     3661 tcaccgcttt gtcgacgctg gcgtcggcac ctgccacata gccgccgagc gggatcagag
     3721 gtttgatctc catataggcg gcatagtttt gctttaacgc gcgggcggat tgctgatgtt
     3781 ccaggctggt gacctggttc atgcagcggc tgatcgactg gccgatatca atggccggat
     3841 agtgccccgc ttccgccagt ttgcgggtta atacgatatg cccgtccagc accgcgcgcg
     3901 cgcagtcgac aatcggatct tgctggtcgt caccttccgc cagcacggtg tagatggcgg
     3961 tcatcgatcc cgcgctttcg ctgttgcctg cgctttcgac cagtttgggg atcatgccga
     4021 atgccgatgg cggataacct ttggtggcgg gcggttcgcc gagcgagagg gcgatttccc
     4081 gctgcgccat cgcgtagcgg gtcagggaat ccaccagcag taagacgtga tgcccgcggt
     4141 cgcgaaacca ggcggcgatg gagtggcaca gttcggtggc tttcagacgc atcagcggcg
     4201 attcatcggc aggcgcgacg acgacgatag actttgccag cccgtccgcg cccagcgagt
     4261 ggtcgataaa ctctttcact tcgcgcccgc gttccccaat cagcccaacc accacgatat
     4321 cggctttggt ctggcgggtg atcatcccca gcagcacgct tttcccgacg ccgctgcctg
     4381 ccattagtcc gacgcgctgg cctttgccga tggtcagcag gccgttgatc gctttcacgc
     4441 ccacgtccag cggttcgtct accgaacgac gagttaaggg attaaccgag ggcggcaacg
     4501 gcggcagaag gtcgttgccg ttcaggcggc ctttgccatc cagcggttcg ccgaggccat
     4561 tcaccacgcg gcctaaccag ctttcgccta tcaggatgtc gtgggctttt tcttccggga
     4621 agacgcgtgc gcctgccatc aggccgaccg gctgtttaaa cggcattaaa taggtgatgt
     4681 cgcgattaaa gcccacggcc tgcgcgtcga tcagcgtgtg attggcactt tcgatgcggc
     4741 acagctgccc ggtcattagc gggcagccga cactctccag caggatgccg ttaaggcgca
     4801 ccagccgccc ggcgacgcgg gccagcggga tcgactccag cgagcgcagg gcgttatcga
     4861 acgcagaaaa atcactcacc ctgcgactcc ggcgtgagtg tttctttcag cgcggtcatg
     4921 cattgttcaa ggcgatgctc gcagccgata tccagttcgg atttgtcggt gatcacccgg
     4981 cattcgcccg gtggcaaatc cggcgaaggc gtcagccccc attcgctgac cttttccggc
     5041 accgcgtcgc gcaggcggtt aaactcttcc gtgctcagca ttacttgcag ggtttccggc
     5101 atcgccggga aggccgccag cgcctcttcc accagcgcca gcaactgcgt gggttgcagc
     5161 gccagttcac aacggatcac ctggcgggtg actttttcca ccagttgcag caaatcttcc
     5221 cgctgtttac gttcgatatg agcgaggaag tcgttaactt tcccggtgat tgtttccagc
     5281 ggctgcgccg cctcaacaaa ctgtttgcgc ccttcctgct gcccggcgag gctgccttcg
     5341 gtgtagccct ggcgtcgccc ctcggcaaaa ccttgctgat gaccttcgct aaagccctgt
     5401 tcctgacctt ccgtcattcc ctgagcaaac cctttttgca gcccttcctg aaaaccgtcc
     5461 atcaactggc gctggtagtc ggcggcgctg atgctggcgg tgagcggttc tccggtcacc
     5521 gcacgctggc gcggcgggaa acggtgcagg cgatagcgcc cgcgcagtgt ttcaatggtc
     5581 atgggttact ccgccgtctg ttccgcaaag agctgtagct gaatttcgcc cgcctcttcc
     5641 agttcacggg cgatgcccat gatttcgcgg cggatctgtt cgatacggct gaccggcacc
     5701 ggccccagtc gcgcggtgat cgcttccagt tgttgcacct gacgttttgg cattaccgcg
     5761 taaatcgagc gccgcaacag ggcttcggtg cctttcagcg ccaccgccca gtcttccatc
     5821 ggcacttcgt cgagcagacg tcgacgtacc tcttcgttct ggcggctgag gataaagaag
     5881 tcgtacatct catcctggag ctgctccagc acctcttcgt cgcgctcgcg catctggtcg
     5941 aggatcacct gctggttacc ctggaagcgg ttgacgatat ccgccgcctg tttgatgcct
     6001 ttcaccttcg agccgtgttc agagagcacc gacagaccgc gttcgatcag cctgtccagt
     6061 tcatccacca cgtcgcggtt gacgtcgttg agtttcgcca cccggtagag aatctcgttt
     6121 tgcaccgatt cgttgagata cgagagcacc gccgccgaga tttccggcgt cagaaacgcg
     6181 aggaacaccg cctgcaattg cagatgttct tcggagatca gcatcgccaa ctggcgcggt
     6241 tcaacccatt gcagtcgcgc catccgcgag cggatctcat cgccgtagat gccgttgatc
     6301 acgctgctgg cgatctcggt gccgagggct ttgttgagaa tgccctgcaa catggatcgc
     6361 gatgcgccgt taatcccgct ctgttcgcgg aactcatcaa agaagttatt gatcactttg
     6421 cgcgccatgc tggttttgac gccggaaagc cgcgccatgt tttcgctcag gcgcaccact
     6481 tcttcgcggc tgagtttttg catcaccgtc gccgccgctt cttcgcccag acacagcagc
     6541 aggatcgccg cctgttccag gtagctgttg ctcttatttg ttgtcaattc gctcattgct
     6601 ggtgatccat tgtctgagaa cttcggctac gcgatcggtg tcgctcatgg ccagtttttg
     6661 caggaactcg agttttacct cgaggccgga actctgcgaa ggcaggctgt catcgccggg
     6721 tagcgacggc aggtcgacgt ttttacgttc ttcttccggc gtggcgatcc acggttcgct
     6781 gacggcttcc ggttctggtg cgataaccgg cgtacgtacc gccgtcagtc gtttcatcag
     6841 cgggcgcacc acaaagaaca gcaacaacag cgccagcagg ccgcagccaa tcaggcgcac
     6901 ccaggccagc acgctgtcat ctttccacag cggaatgaca ggctcgaccg gggccacctg
     6961 cggcacaaag ttaagcagtg aaagcgtcag gttatcccca cgttgcgcgt caataccggc
     7021 ggcattgttc agcaacgcgg tgagctgtgt ggtctgctcc ggcttccagt ttttcagcgc
     7081 cggagcgttt tgattgagca ccaccgccac gttgagtcgt ttcaccgcaa agccgggatg
     7141 ctgaatatgc tggacgctgc ggtcgtaaga gtaatcgcgc ttgctttcgc tgtgtttgga
     7201 taacgcttcc ggcgagcgat tttcttccgt gccgttggtc atctgattcg ccgcaaccgg
     7261 cggacggttg ctgagcgaac cgggcacgcc catcgccacc tgattggtgt cgctgtccag
     7321 cacgctctct tcgcggttga ttttcggcgc gtcgccgtag tgttcctggg tttcgtcgat
     7381 agtgctgaga tcgagatccg gcatcacgct gacgcggtaa ttccctgtgc caaccagaga
     7441 atccagcacg tttgccacgc tggcgcgggt tttgtcctga atatctttga ggatctgatc
     7501 gcgtttacgg gtagcggcag aaaccgcctc gcccgcgcca ataccgtcgg tcagcagatt
     7561 tcccgcctga tcgacgacgc tgactttgct ggcgtgcagc cccggaatac tgccggaaac
     7621 cagatgcaca atggcgttca cctggtccat attcagcttc gcgccatagt gcaggcgtac
     7681 taccaccgag gcgctgtttt gcggcacatc gctgaccaca aacgagcttt cttcgttgag
     7741 cgccagatgc acccgcgcgc tctccaccgc gtccagcgac ataatgctct gcgccagctc
     7801 cccttcgagg ctgcgtttat agcgcacgtt ctgcacgaac tggctgctgc cgagcacttc
     7861 gtctttgtcc atcagctcgt agccgctggg caatatcgcc tgcacgcctt tcgcggcaag
     7921 ggtcatacgc gttttcgaca gttcatcttc cggcaccaga atctgcccgc tctgcgggtc
     7981 gatgcggtag ctgagctttt cgccgtccag cacggtgaca atctgcgaaa cgggcaggtt
     8041 ttcctggcgg ccgtagagcg agacgtagcc gcgattgccg gtccacagca cgctgacgat
     8101 aatcgccgtt gccgcaacgg caagccccac tcccgccatc agcgcccagc gtttgttatc
     8161 tgcaagacgc agtcgaaacg ccggaaacgc ctgggttaac ttctttattt gtgcattcat
     8221 agggttagat cgacatactc atcaggtcat taaacccggt ggcgattttg ttgcgcacct
     8281 gcaccagcgc ggagaaagag agcgacgcct gctggctggc gatcatcgca cctgccaggt
     8341 catcgctttt gcccatgtcg atagccgttt gctgctgctc cgccacctgc tggaactggt
     8401 caacgtgacc gagcgcgccg ctcataatgc ggttaaacga aacgtcaggg tcggtggaac
     8461 tgaaggtcat ggcgggcagc accggtgcct gggcgttggc ctgcatccgc tgcacgtcct
     8521 gcatgatttg cgcctgcatg gtgctgttga ttgtggtaat gctcattttt aacttccggg
     8581 tcaggagaaa acttcgatac cttgcttgcg catggaggcc agtcgatagc gcagtgcgcg
     8641 cggggtaatg cctaacaggt cggcgatttt gctgcggttg ccctgatatt tacgcatcag
     8701 gtcggcgata tattgatact gggcgcttcg tccgtgctgg cctaaatcgc tggtttcggc
     8761 aatgtgtacg gcgggctgaa caggccattg cagttcgctg cgatcggcaa cgtccgtttc
     8821 cggcaagcct aagacatccg ggtaaatcac gccgtcgcgg ttcaagatca ttccccgctg
     8881 aatggcgttt tccagctggc gcacgttgcc gggccagcgg taattcagca gcgcccggcg
     8941 ggtagattct gaaagggtga tatttttcac cagcaccgtg gagtattttt taataaatga
     9001 ctctgccagc ggaataatat cgttcagacg ttcgcgcagc ggcggcatgg taataggaat
     9061 aaccgccaga cgataataga gatcttcacg aaaacgcccg gcggcgactt cctgctcaag
     9121 gtttttattg gtgcaggcaa tcaggcgaaa attgagttta atttgtcggt tgctgcctaa
     9181 tcgctcaacc tgttgttcct gcaatacgcg taatattttg gcctgtaatg ccagcggcat
     9241 atcgccaatt tcatcgagca ataaggtgcc gttattcgcc agttccattt ttccaggcac
     9301 gctggcaatt gcgccggtaa atgcaccttt gtcgtagcca aataaggtgg cttccagcat
     9361 attttccggg atcgctgcgc agttcacgcc gatatagggc gcgttatcat tttcaccaaa
     9421 ggctacggtg tgaatatatt ttgccacgca ctctttgccc gcaccggttt cgccctgaat
     9481 gagcaccggc acgttaaagg cggcgacacg ctttgccaga gtaaaagcgt taatgctgga
     9541 agcggcagtt gcaataagtt cagacatagg gtgaacgata taagtagcga cagaaaacag
     9601 gatgttctga agcgtatcat cggcgggatt aacaagttcg aattaaacga taataaaaat
     9661 attatttgtt gaataacaat gagttatagc cgtaggccgg ataaggcgtt tacgccgcat
     9721 ccggcaattg tgcgccgatg cctgatgcga cgctggcgcg tcttatcagg cctacggttc
     9781 gtgcaatgtt ttatgacgat gcccgacatc tcacccttta ataatttaat tattttttca
     9841 ctctattaat aatttgcccc tttcacgccc tctctattta atacctcacc ttgtgtcact
     9901 gccggatttg cgtgcacaaa aacgcccata ccattacagc agagaaagta agccatgctg
     9961 aagtacagta aaacgccagg catctttaaa ttagaaggta atcggctcgg acgcccctac
    10021 catcgcctgc cgaccctgtt tacggggaat tttgacgtga ttgattcgca tctgggcagc
    10081 tactttctca aaaagcatcg cagcaatatc accttaaaaa agattggctg cgaaatggat
    10141 attattaata aaaacgccga aatgatggtt tcccaggtcg ggcatctggc gtttgatatc
    10201 gatcgctcct tgctgttaat gctgctgggc aatttttatg gtctggagtc ctctcttgaa
    10261 gaggcgaaag cgcataacgg gctgccgacc aaaaccgaaa cgcggttaaa aaaccgactc
    10321 gcgctggata tctgcacgct gatatttaat ctgcaaacct ccggtattgc gctgaaatta
    10381 aagctggaca gcagcacggt aattacccac tgggcgtatc aattaacctt taccctcgcg
    10441 ggcgatgaag agagcagttt tcgtattttg ctcgacgacg cccacaccga ttttatttta
    10501 aatctgatcc gccacagcga gcacagtaaa ccgcagcagg tggcgaaatc cgtcaataaa
    10561 ccggccctga ttaaagagat catccgctcc ctgccgctga ccctgaacgt caaaatcgcc
    10621 gagctatcga tgaatgtcgc ggatctcacg cagataaaag ccggggatat tttacccatc
    10681 tcgctgggag aaacgttccc ggtggcgatt gggcaatctg aattatttag cgccctgatt
    10741 gtggaagaca aagacaaact gtttttgtcg gagctggcgg gcaaaaatga gaatagccat
    10801 gagtaagcaa gaagatattt tagccgggga ttttggcctc accgatgaag ttgcgcctgt
    10861 cgcaaaatcc gccgacgctg aaacgctggt cacccgcctg gaagaccgtt tttccgactc
    10921 gatgacgctg ctaaaacgca ttccggtgac cttaacgctg gaagtgtcgt cggtggagat
    10981 catgttagcc gacctcctta acatcgacga cgacacggtg attgagctgg ataaactcgc
    11041 cggggagccg ctggatatta aagtgaacaa tatcctgctg ggcaaagcgg aagtggtggt
    11101 ggtcaacgag aagtacggcc tgcgcgtgct ggaatttaac acccgcgata tcaacgacct
    11161 ggcaccatga agcgccgcac tcaactgacg ctgggccttg ggctgctggc gcttgcgccg
    11221 ctggcgttgg cccagggcgg cgatattgcg ctgcttaacg tggtcaccca cggcaacaca
    11281 caggagtaca gcgtcaaaat ccaggtgctg attctgatga ccctcgtcgg tttgctgccg
    11341 acgatggtgc tgatgatgac ctgctttacg cgctttatta tcgtgctgtc gctgctgcgc
    11401 caggcgttgg ggctgcaaca aacgccgccg aaccgtattc tgattggcat tgcgctctcc
    11461 ctcaccatgc tggtgatgcg ccccatctgg ctcaatattt acgaccacgc agtagtaccg
    11521 tttgaaaatg atcagataac cctgaccgat gcgttaagca ccgccgcgac gccgctgaaa
    11581 cgctttatgc tggcgcagac cgacaaaaaa gcgatggcgc aaatcatgac catcggcggc
    11641 gcgaagggca atgccgccga tcaggatctc tctatcgtgg tgcccgccta tgtgttgagc
    11701 gagctgaaaa ccgccttcca gattggcttt atgatttaca tcccgtttct ggttatcgac
    11761 ctgattgtcg caagcgtatt gatggcgatg gggatgatga tgctgtcgcc gctgatcgtt
    11821 tcgctgccct ttaagctgat gctgtttgtg ctgatcgacg gctggtcgct gaccatcggc
    11881 acgctcacca ccagcattcg cgggctgggg ctgggctgat tatgctgacc gtagacgtag
    11941 ccgccgacat tgtcgccagc ggcattaaag tggtgatttt gctggtttcg gtactggtcg
    12001 tgccaagcct gctggttggc ctgctggtga gcgtgttcca ggcggtgacg cagattaacg
    12061 aacagaccct gagctttttg ccaaggctga ttgtcacgct ggtggtgctg ggcgtgtgcg
    12121 gcaaatggat gattatccag ttgcatgatc tctgcattca tctgtttacc caggcagccc
    12181 tgctggtgca ttaagatgcg cacaagcgac gttacgcaac tgatggatct ggcgctgggg
    12241 ctgtggtttc ccttcgtgcg cattatggcg tttctgcgct atgtgccggt gctggataac
    12301 agcgccctga ccgtgcgcgt gcggattatt ttgtcgctgg cgctggcaat catcatcacg
    12361 ccgttgatcc cccatcccat tccccacgac ctgctttcac tcaacagcct gatcctgacg
    12421 gtggagcaaa ttttatgggg catgttattc gggctgatgt ttcagtttct gtttctggcg
    12481 ctgcaactgg ccgggcaaat tctctcgttc aatatgggga tgagcatggc ggtgatgaac
    12541 gacccaagca gcggcgcatc gaccaccgtg cttgcggagt taattaacgt ctatgcgatc
    12601 ctgctgtttt ttgccatgga cgggcatctg ctgctggtga gcgtgctgta taagggcttc
    12661 acctactggc cgattggcaa cgcgctgcat ccgcaaaccc tgcgcaccat cgcgctggcc
    12721 tttagctggg tgctggcgtc cgcatcgctg ctggcgctgc caaccacttt tatcatgctg
    12781 attgtgcagg gctgctttgg cctgctcaac cgcattgcgc cgccgctgaa cctcttttcg
    12841 ctcggctttc ccatcaacat gctggcgggg ctggtctgtt tcgccaccct gctctacaac
    12901 ctgccggatc actatctgca tctggcgaat tttgtgttgc aacagctcga cgcgctgaaa
    12961 ggtcactatg gcggatagca gcagcgaaga aaaaacagag aaaccctcgg cgcaaaagct
    13021 gcgtaaagcg cgtgaagagg ggcaactgcc gcgctcgaag gatatggggc tggcggcgag
    13081 cctgtttgcg gcgtttgtgg tgatctccag tagttttccg tggtatgccg attttgtgcg
    13141 cgagagtttt atcagcgtgc atcagtatgc gcaggagatt aataacccgg aagtgatcgg
    13201 tcagtttctg cgccatcacc tgttaattct cggcaagttt attctcacgc tgctgccgat
    13261 gcctgccgcc gcgctgctct cttcgctggt acccggcggc tggctattct taccgaaaaa
    13321 aatcctcccg gatttcagca aaatcagccc gttaaaaggc attgggcggc tgttttccag
    13381 tgagcatctg gcggaaacag ggaagatgac ggtgaagtcg gtagtggtgc tggtgatgct
    13441 gtggattagc ctgcgcaata actttgccgc ctttctcggc ctgcaggcgc taccgttcaa
    13501 actggcgatg aatgagggtt tgtcgctcta cgccagcgtg atgcgcaact ttgtcatcct
    13561 gtttatcttc tttgcgcttc tcgacgtacc gctggcgaaa gccctgttca ccaaagggct
    13621 gaagatgacc aaacaggagc tgaaagaaga gtataaaaac caggaaggca agccagaagt
    13681 gaaggcgcgc gtgcggcgac tgcaacggca actggcgatg gggcaaattc gcaaagtggt
    13741 gccgaaagcc aacgtggtga tcaccaaccc gacccactac gccgtggcgt tgcagtatga
    13801 ccagtcgcgc gccgccgcgc cgtttgtggt ggcgaaaggc accgatgaaa tcgccctcta
    13861 tattcgccag gtcgccgctg agaatcaggt ggaagtggtc gagttcccgc ggctggcgcg
    13921 ctcggtttac tacaccaccc aggtgaatca gcaaattccg tttcagcttt accgggccat
    13981 cgcccacgtt ctgacctacg tcctgcaaat gaaacactgg cgcgagggcg cacagccgcg
    14041 cccggccctg aacagagata tttccattcc caaagaggtg cttaaactgg atggcgaaaa
    14101 caactaaatc gttcctcgcg ctgctgcgcg gcggcaatct cggcgtgccg ctggtgatac
    14161 tttgtattct ggcgatggtt attttgccgc tgccgcctgc gttgctggat attctgttca
    14221 ccttcaacat tgtgctggcg gtgatggtgc tgctggtggc ggtgtcggcg aaaaggccgc
    14281 tggaattcag cctgttcccg accattttgc tgatcaccac cttaatgcgc ctgacgctta
    14341 acgttgcttc tacgcgcgtg gtgctgctgc acgggcatct cggcgcgggc gcggcgggta
    14401 aggtgattga gtcgtttggt caggtggtga tcggcggcaa ctttgtcgtc ggcttcgtgg
    14461 tgtttatcat cctgatgatc atcaacttta ttgtcgtcac caaaggggcc gagcgtattt
    14521 ccgaggtttc tgcccgcttt accttagacg cgatgcccgg caaacagatg gcgattgacg
    14581 ccgatcttaa cgccggattg atcaaccagg cgcaggcgca aacccggcgt aaagatgttg
    14641 ccagcgaggc cgatttctac ggcgcgatgg acggggcatc gaagtttgtg cgcggggacg
    14701 ccatcgccgg gatgatgatt ctggcgatca acctgatcgg cggcgtctgt atcgggatct
    14761 tcaaatacaa cctgagcgcc gatgctgcct tccagcagta tgtgctgatg accatcggcg
    14821 acggcctggt ggcgcagatc ccttccctgc tgctctccac ggcggcggcg attatcgtca
    14881 cccgcgtcag cgacaacggc gatatcgccc atgacgtgcg ccaccagctg ctggcaagcc
    14941 cgtcggtgct ctacaccgct accgggatta tgtttgtgct ggcggtggtg ccgggaatgc
    15001 cgcatctgcc gtttttgctg ttcagcgccc tgcttggctt taccggctgg cggatgagca
    15061 aacgcccgca ggcggcggaa gcggaagaga aaagtctcga aacgctgacc cgcaccatca
    15121 ccgaaaccag cgagcaacag gtcagttggg aaaccattcc gctgatcgag cccatcagct
    15181 taagcctcgg ctacaagctg gtggcgctgg tggacaaagc ccagggcaac ccgctcaccc
    15241 agcgtattcg cggcgtgcga caggtgattt ctgacggcaa cggcgtgctg ctgccggaga
    15301 tccgcattcg ggaaaacttc cgccttaagc ccagccagta cgctattttc atcaacggca
    15361 ttaaggctga tgaagcggat attccggcgg ataaactgat ggccctgccc tccagcgaaa
    15421 cctacggcga gattgacggc gtgctgggga acgacccggc gtatgggatg ccggtcacct
    15481 ggatccagcc tgcgcagaag gcgaaggcgc tgaatatggg gtatcaggtg atcgacagcg
    15541 ccagcgtgat tgctacccat gtgaacaaga ttgtgcgcag ctatattcct gatttgttta
    15601 actatgatga tattacgcag ttgcataacc gtttggcgtc gatggcaccg cgcctggcgg
    15661 aagatttaag cgcggcgctc aattacagcc agttgctgaa agtgtaccgg gcgctgctga
    15721 ccgaaggcgt ttccctgcgc gatatcgtca ccatcgccac cgtgctggtc gccagtagcg
    15781 cggtgaccaa agatcatatt ctgctggcag ctgatgtgcg cctggcgctg cggcgaagca
    15841 ttacccatcc gttcgttcgc aagcaggagc tgacggtgta tacgctgaat aatgagctgg
    15901 aaaatctgct gaccaacgtg gtgaatcagg cgcaacaggg cgggaaagtg atgctcgaca
    15961 gcgtgccggt cgatccgaat atgctcaacc agttccagag tacgatgcca caggtgaaag
    16021 agcagatgaa agcggcgggg aaagacccgg tgctgctagt gccaccgcag ctgcgccctt
    16081 tgctggcgcg ttatgcaagg ttgtttgcgc cggggctgca tgtgctgtcg tataacgaag
    16141 tgccggatga gctggagttg aagattatgg gggcgttgat gtaagtatcc agcattggtg
    16201 cacaactgcc ggatgcggcg tgaacgcctt atccgtccta ccgttcaggc acaagtttgt
    16261 aggcatgata agacgcgcca gcgtcgcatc aggcatctgc gcataactgc cggatgcggc
    16321 ataaacgcct tatccggcct accgatccgg cacaggtttg taggcatgat aagacgcgcc
    16381 agcgtcgcat caggcatctg cgcataatta taaaatgata cgctcgccag catttatatc
    16441 cgttacgtcc cccgcccaat cgatgggata taaccctcgc gcgacatcgc gatggaacgt
    16501 tgagaatggc caatcactca cttgctttac ccaaccatgc tttactggat ttatataaat
    16561 ataatcaaca tgatgcctat aatctttggt attgcggatg gtgtgctccc aaaaacgtgg
    16621 ttgccagata tttttcaatc cacaagcatg ggtaaattgc tttttaattt cccgccagcg
    16681 agaggaaaaa tcatcatcgc cttcaggtaa tgtccatatg caatgcatat gctctggcaa
    16741 aacgacccag gcgttgattt caaaaggcct gtctcgctta actttaataa tggcgttacg
    16801 gagcatctgg tactgggtgg tcaagagttg gcttcgacga tggcgtaaat tcaccgtgaa
    16861 aaaccaggtt ccccccttga tgtaataacg acgatattca gacatggcga ctcctttttc
    16921 tcaacggtaa gaagaaaagg aaaggtcggc aatgaggtga tggaataaca ggaaagcggc
    16981 tcgcaaaaac gcacagattt gtaggcatga taagacgcgt cagcgtcgca tcaggcatct
    17041 gcgcacaacc gccggatgcg gcgtaaacgc cttatccggc ctacaaaatc gtctcttccg
    17101 tcaaaatcct gcgcgcgccc aaaaaatggt cctgccagaa aggttcggct aatcggctta
    17161 tccgaatggt ttcgccggta cgtggcgact cgataaattg cccatcgccc aaatacacgc
    17221 ccatatgatc ggctatctcg cggctgtgga tatggaaaaa cagcaaatct ccccggcgca
    17281 ggtcgttatt cgccacaatc gttgcccgat gatagtgata catctcattg gccgtgcgcg
    17341 ggagcttcgc ctcaaggatc ttgttgtagg cataaaaaac caacccgcta cagtcgaagc
    17401 cttgatcggg ccgcgtgccg ccccagacgt aaggcttgcc aagctgttgc tccagacggt
    17461 gaatcgccac ttcggtgatg ttatgcagat ggtcgctgct taaaaagtga ttgttctccg
    17521 ccagcgcctg ccagcgtcgg tcgctcgcct tgagcggcgc aggaaaccat tctggatgct
    17581 gttttatctg ctcccggttg tgctggcgta gcgcccttct gctttcggca ttgccttcca
    17641 caatatagct ggcctgtttt ttcagatggg tttggtactg ttttaataaa cgcgcacggt
    17701 tttgcatccg ctggcgggcg gcgtagctga aagagatatg tgatgtggtg gcgctggcga
    17761 ataaagggag caaaacgcat aacgaaaaca cgcctggatg cagaaatctg ccgagcaaga
    17821 acgaagacat gaaagacata aaccggctga atgaaggtga gaattttaaa agaccggagg
    17881 agtaaacggg attattaatt gcttatttgc atattactat tgaggtttca taatcttagg
    17941 aaataaatga tacatgcgct tttcggacta ttgaaaactg cccctgtgct aaatttgtaa
    18001 accctgcgcc gacaggcttg cgtaaaactc ttttggtgat aatgtgagcc ttccaaatcc
    18061 agactattcc gatttgggat ccattttgcc gtcctggcgt gtgtaaccgt tttatcaagg
    18121 aataagcagt atgcgtaaaa tcgcattaat tcttgcgatg ttgttgatcc cgtgcgtttc
    18181 gtttgccggt ttgctgggca gcagtagctc gaccacgcca gtgagcaaag agtataagca
    18241 gcagttgatg ggatcccctg tctacatcca gatcttcaag gaagaacgta cgctcgatct
    18301 ctacgtcaaa atgggcgagc aatatcaact gctcgacagc tataaaatct gtaaatattc
    18361 cggcggctta gggccaaaac agcgtcaggg cgatttcaaa agcccggaag ggttttatag
    18421 cgtccagcgt aatcagttaa aaccagacag ccgttactac aaagcgatta atattggttt
    18481 ccccaatgcc tatgaccgtg cgcatggtta tgaagggaaa tatctgatga tccacggcga
    18541 ttgtgtttcc atcggctgct acgcgatgac caatcagggt attgatgaga tcttccagtt
    18601 cgttactggt gcgctggtgt ttggtcagcc gagcgtgcag gtgagtattt acccattccg
    18661 catgaccgac gccaatatga agcgccataa atattccaac tttaaggact tctgggagca
    18721 gctgaagccg ggctacgact actttgagca aacccgtaag ccaccgaccg tttctgtggt
    18781 caatggccgt tacgtggtca gcaagccgtt aagccacgaa gtggtgcagc cacaactggc
    18841 atcaaactac acgctccccg aggcaaaata agcgccattc gcctggcata attttttgcc
    18901 aggtttcatt gcccgtcagc ggctgtgtcg caatcaccgt gaccacatca ttcggtgtgg
    18961 tctgcgagct gaagtcgatt tccacatcct gatccagcaa cgttgccacg ccaaacggcg
    19021 cgcggcgggt gatccagtgt aaattagtcg agcaatacgc cattacatag cgaccgtccg
    19081 aaagcagcat gttgaaaacg cccttcgccc gcagttcatc cgccagtgag gcgatatatt
    19141 taaacactgc cgccatgttg cccggcgtgc gcgggtaacg ctgcgttaat ttatgcagga
    19201 gccagcaaaa ggctttttcg ctgtcggttt caccgaccgg gcggaagttg ccggtttcca
    19261 gtgatttgta gcccgtcagt tgcccgttat gggcataagt ccagttacgc ccccataact
    19321 cgcgggtaaa cgggtgagta ttttccagcg ccacctcgcc ccgattagcc tggcgaatat
    19381 gagccaccac cgaacaggat tttatcgggt agtcctggac aagtttggcg atgggggaat
    19441 taaagctggg ttgtggatct ttaaatgtgc gacagccttt accttcgtaa aaggtaatgc
    19501 cccagccatc tttatgtggc ccggttccac caccacgctg tacaagcccg gtgaaactaa
    19561 agcagatatc ggtagggacg ttggcgctca tcccgagcaa ttcgcacatg ggtaacctcc
    19621 acaacagcgg gagcgcatgg cccccggcga agattgattt aagaattttc aggtcggatg
    19681 cggcgtgaac gcattattcg gcctacggtt cgcgtacaga tttgtaggca tgataagacg
    19741 cgtcagcgtc gcatcaggca tcagcgcaca aatgccggat gcggcgtaaa cgtcttatcc
    19801 ggcctacgcc agacttactt aaccatctct ttttcaatca actggatcag gatatggatc
    19861 actttaatgt gaatctcctg aatgcggtcg gcataaccaa agtgcggtac gcgaatttcg
    19921 atatccgccg tgccagccat tttgccgccg tctttaccgg tcagggtgat cactttcatc
    19981 cccttctcac gcgccgctgc gatcgctttg atcacgtttg cagagttacc ggaggtggag
    20041 atccccagca gtacatcgcc ttcgcgacct accgcttcaa cgtagcggga gaaaatatca
    20101 ttgaaaccga aatcattacc gacgcaggaa atatgactaa cgtcagaaat agcaatcgcc
    20161 gggtagcccg gacggttttc acggtagcga ccggtcaact cttcggcaaa gtgcatagcg
    20221 tcgcaatggg aaccgccgtt gccgcaggaa agcactttgc caccggcttt aaagctgtct
    20281 gctaacagga ccgccgcgcg ctgaatggcg tgaatattgg cgtcatcttt taaaaagtta
    20341 gccagcgttt ccgccgcttc gttcagttcg ttacgaataa gatcctggta catgaggata
    20401 tccttcagca taaatgtaat agacaaaatg cagtgtaccg gataccgcca aaagcgagaa
    20461 gtacgggcag gtgctatgac caggactttt tgacctgaag tgcggataaa aacagcaaca
    20521 atgtgagctt tgttgtaatt atattgtaaa cacattgcta aatgtgttta catccactac
    20581 aaccatatca tcacaagtgg tcagacctcc tacaagtaag gggcttttcg ttatgatgat
    20641 tttgagtatt ctcgctacgg ttgtcctgct cggcgcgttg ttctatcacc gcgtgagctt
    20701 atttatcagc agtctgattt tgctcgcctg gacagccgcc ctcggcgttg ctggtctgtg
    20761 gtcggcgtgg gtactggtgc ctctggccat tatcctcgtg ccatttaact ttgcgcctat
    20821 gcgtaagtcg atgatttccg cgccggtatt tcgcggtttc cgtaaggtga tgccgccgat
    20881 gtcgcgcact gagaaagaag cgattgatgc gggcaccacc tggtgggagg gcgacttgtt
    20941 ccagggcaag ccggactgga aaaagctgca taactatccg cagccgcgcc tgaccgccga
    21001 agagcaagcg tttctcgacg gcccggtaga agaagcctgc cggatggcga atgatttcca
    21061 gatcacccat gagctggcgg atctgccgcc ggagttgtgg gcgtacctta aagagcatcg
    21121 tttcttcgcg atgatcatca aaaaagagta cggcgggctg gagttctcgg cttatgccca
    21181 gtctcgcgtg ctgcaaaaac tctccggcgt gagcgggatc ctggcgatta ccgtcggcgt
    21241 gccaaactca ttaggcccgg gcgaactgtt gcaacattac ggcactgacg agcagaaaga
    21301 tcactatctg ccgcgtctgg cgcgtggtca ggagatcccc tgctttgcac tgaccagccc
    21361 ggaagcgggt tccgatgcgg gcgcgattcc ggacaccggg attgtctgca tgggcgaatg
    21421 gcagggccag caggtgctgg ggatgcgtct gacctggaac aaacgctaca ttacgctggc
    21481 accgattgcg accgtgcttg ggctggcgtt taaactctcc gacccggaaa aattactcgg
    21541 cggtgcagaa gatttaggca ttacctgtgc gctgatccca accaccacgc cgggcgtgga
    21601 aattggtcgt cgccacttcc cgctgaacgt accgttccag aacggaccga cgcgcggtaa
    21661 agatgtcttc gtgccgatcg attacatcat cggcgggccg aaaatggccg ggcaaggctg
    21721 gcggatgctg gtggagtgcc tctcggtagg ccgcggcatc accctgcctt ccaactcaac
    21781 cggcggcgtg aaatcggtag cgctggcaac cggcgcgtat gctcacattc gccgtcagtt
    21841 caaaatctct attggtaaga tggaagggat tgaagagccg ctggcgcgta ttgccggtaa
    21901 tgcctacgtg atggatgctg cggcatcgct gattacctac ggcattatgc tcggcgaaaa
    21961 acctgccgtg ctgtcggcta tcgttaagta tcactgtacc caccgcgggc agcagtcgat
    22021 tattgatgcg atggatatta ccggcggtaa aggcattatg ctcgggcaaa gcaacttcct
    22081 ggcgcgtgct taccagggcg caccgattgc catcaccgtt gaaggggcta acattctgac
    22141 ccgcagcatg atgatcttcg gacaaggagc gattcgttgc catccgtacg tgctggaaga
    22201 gatggaagcg gcgaagaaca atgacgtcaa cgcgttcgat aaactgttgt tcaaacatat
    22261 cggtcacgtc ggtagcaaca aagttcgcag cttctggctg ggcctgacgc gcggtttaac
    22321 cagcagcacg ccaaccggcg atgccactaa acgctactat cagcacctga accgcctgag
    22381 cgccaacctc gccctgcttt ctgatgtctc gatggcagtg ctgggcggca gcctgaaacg
    22441 tcgcgagcgc atctcggccc gtctggggga tattttaagc cagctctacc tcgcctctgc
    22501 cgtgctgaag cgttatgacg acgaaggccg taatgaagcc gacctgccgc tggtgcactg
    22561 gggcgtacaa gatgcgctgt atcaggctga acaggcgatg gatgatttac tgcaaaactt
    22621 cccgaaccgc gtggttgccg ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta
    22681 tctggcacct tctgacaagc tggatcataa agtggcgaag attttacaag tgccgaacgc
    22741 cacccgttcc cgcattggtc gcggtcagta cctgacgccg agcgagcata atccggttgg
    22801 cttgctggaa gaggcgctgg tggatgtgat tgccgccgac ccaattcatc agcggatctg
    22861 taaagagctg ggtaaaaacc tgccgtttac ccgtctggat gaactggcgc acaacgcgct
    22921 ggtgaagggg ctgattgata aagatgaagc cgctattctg gtgaaagctg aagaaagccg
    22981 tctgcgcagt attaacgttg atgactttga tccggaagag ctggcgacga agccggtaaa
    23041 gttgccggag aaagtgcgga aagttgaagc cgcgtaaatg aataacggag ccgaaaggct
    23101 ccgtttcttt atccgctaat tatttaaaat taaagccatc cggatggttt tccaggctgc
    23161 cggtcaacgc cgcgaacaac accgttttac catcaatcga aagcgcatcg ttcacattca
    23221 gccaggtgag tttctcttgc gacgttttct catcaatagt cgagaacagc cccgtcatct
    23281 gattagattt ctcggaccac atcacagcga tacgttgcga gccacagtca tgcggtttgc
    23341 acgcgctcat cacctgatac gtctcatctc ccaacgttac ggtttgtgcg ggagtataag
    23401 taccgccttt catcacccag gcaggcagct tatgcccttg taccatctga ttaaatgcag
    23461 ctttggtggt ttcgcccttt gcaaggctgc taatggttaa atcatcctgc gccattgcac
    23521 tggtggcgat gaccagagcg gcgactgtcg ttattgcctt aaacatcatt cctcccgagc
    23581 ttatcctgcc catgttatta acctcctaaa gcctggcgat aacacgataa tttgcaaatc
    23641 agatatttcc aatatcagca tttgttatca atttattctg cgctaaggca actgcctgat
    23701 aaggtgttaa agttataaaa cccttcgcat aaaggagttg tacaccgtgc ctggtttgaa
    23761 gattacgctt ttgcagcaac cactggtgtg gatggatggt cctgccaacc tgcgtcattt
    23821 tgatcgtcaa ctggaaggta ttaccgggcg cgatgtgatc gttctaccgg agatgtttac
    23881 cagcggcttt gccatggaag cggcagcttc gtcgctagca caagatgacg tagtgaactg
    23941 gatgacagct aaggcgcagc agtgcaatgc gctgattgca ggcagtgttg cattacaaac
    24001 ggagtctggt tcggttaacc gctttttgct ggttgagccg ggcggcacgg tacattttta
    24061 tgataagcgt catctgttcc gcatggcaga tgagcatcta cattataaag cgggcaatgc
    24121 gcgagtgatt gtggaatggc gcggctggcg tattttgccg ctggtgtgct acgacttacg
    24181 ttttcctgtg tggtcgcgca atctcaacga ttatgacctc gccctgtacg tcgccaactg
    24241 gcctgctccg cgctctctgc actggcaggc attgctgacg gcccgcgcga ttgagaatca
    24301 ggcgtatgtg gcgggatgca atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg
    24361 tgacagccgg gtgattaatc cacaaggcga gattatcgct actgccgacg cgcatcaggc
    24421 aacgcgcatt gatgcggagc tgtcgatgat ggcattgcgg gaatatagag aaaagtttcc
    24481 ggcatggcag gatgcggatg agtttaggtt gcggtgagtg ggggttgtgt ttaaggctgg
    24541 gggagagtcg gggtaagact acgaaagccc gctctccgca aggactgacg ccagatagtt
    24601 tctgtccata gctgcttttc gcatcttacg tcttaaccct gccttgaata ccttatcatt
    24661 cgtcaaaata ttaatagcga tgtgccgtat ccctgaaaat aattctgcgg cgtttcctct
    24721 tcttattttg cagtcgtctt cattcattac cacgtccaga cgccagtgca gcttattctc
    24781 cacgtgccag tggtttcgga ttgctgtggc gaacttttct gcggttaaat cagcagaact
    24841 gatataatat ctgaccgtca tttctggctc tttcttttgt tctgctatta ttgaccgaaa
    24901 ggagactgcc atgcataatt tcttcagtcc tttccattca aacgtgaaat caataagttc
    24961 atcagggaca tcgcaaacaa tatgaagacg gatttcttct ctgccgtgac tcttttcact
    25021 cattgcgtaa ctgtcatgct ctggattatt taattctttc agcggaaatt tttcctcaaa
    25081 ggctttattt agccgcccct ggtttccttt taccgcgaat aaataatcac ctccctgt
//
LOCUS       DAJOSY010000064        25093 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJOSY010000064 DAJOSY010000000
VERSION     DAJOSY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.064"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            78..761
                     /locus_tag="OHN27_002718"
     CDS             78..761
                     /locus_tag="OHN27_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCQ2287127.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIQEGKPESSLLKQEEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSIPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMTSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPAA
                     DNRQKRSKK"
     gene            805..5829
                     /locus_tag="OHN27_002719"
     CDS             805..5829
                     /locus_tag="OHN27_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCQ2287128.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     LLSSFGALADNYDGQGVDYGDGSASDGWVAIGKGAKANIFLNNAGASTALGYDAIAEG
                     QYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSFGQYSMALGRYSKALGRL
                     SIAMGDSSKADGANAIALGNAAKAAGIMSIGLGDNANASQDYAMALGAESEAAENATA
                     IGNKAHAKGVNSIALGNGSQALADSAIAIGQGNKANGADAIALGNGSQSSGLNAIALG
                     KASVVTGDNSLALGSNTNANGINSVALGAGSIADQDDSVSVGSDSLQRKIVNVKNGTI
                     KADSHDAINGSQLYAISDSVAKRLGGGSSVNVDDGTVKAPTYNLKNGNKNNVGDALTV
                     LDQFTLQWDQNRDKYSAAHGSSTASVITDVADGAVSDSSKDAVNGSQLKATNDDVETN
                     TTNIATNTGNIATNTANIATNTTNITNLTDTVGDLKDDALLWNGTAFNAAHGTETTST
                     ITNVKAGTLSDDSTDAVNGSQLKDTNDNVATNTTNIASNTANIATNTSNIADNTANIA
                     TNTSNIADNTANIATNTSNIAGNTANIATNTTNIAANTTSINSLNTSVDALEQDAMLW
                     NGTAFNAAHGTETTSTITNVKAGTLSDDSTDAVNGSQLKATNDNVATNTTNIASNTAN
                     IATNTANINTLNTSIDTLEQDAILWNGTAYSAAHGTETASTITNVKAGTLSENSTDAV
                     NGAQLNATNANVATNTTNIATNTASINTLNTSIDALEQDALLWDGTAFSAAHGANKDA
                     SKITNVLAGTVSSASTDAINGSQLHGLSSSIATYLGGGATVSDSGVFSGPTYNIDGND
                     YTNVGAALDAINTSLSDSLGDALLWDSTTGAFSAKHGSTASVITNVADGAVSDSSSDA
                     VNGSQLYDVSNSVVDVLGGGAGVNTDGSISAPTYTIANTDYDNVGDALNALDTTLDDA
                     MLWDATAGENGAFSASHDGSASKITNVAAGTISDTSTDAVNGAQLHGVSSSVAEALGG
                     GAAVNSDGSISAPTYTIADTDYTNVGDAMNAIDSTLDNALLWDAAAGENGAFNASHDG
                     KASVITNVANGQINETSTDAVNGSQLNATNMLIQNIAGDTSESYITENGEGINYVRTN
                     DSGLVFEDASATGVGATAVGYNSVASGDSSVAIGQNSSSTIESGIALGSSSVSNRVIL
                     QGSRDTSVTEDGVVIGYNTSDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPSKYFHANSTEEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVTQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6197..7852
                     /gene="lldP"
                     /locus_tag="OHN27_002720"
     CDS             6197..7852
                     /gene="lldP"
                     /locus_tag="OHN27_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCQ2287129.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7852..8628
                     /gene="lldR"
                     /locus_tag="OHN27_002721"
     CDS             7852..8628
                     /gene="lldR"
                     /locus_tag="OHN27_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCQ2287130.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8625..9815
                     /gene="lldD"
                     /locus_tag="OHN27_002722"
     CDS             8625..9815
                     /gene="lldD"
                     /locus_tag="OHN27_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCQ2287131.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10001..10474
                     /gene="trmL"
                     /locus_tag="OHN27_002723"
     CDS             10001..10474
                     /gene="trmL"
                     /locus_tag="OHN27_002723"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCQ2287132.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10527..11348)
                     /gene="cysE"
                     /locus_tag="OHN27_002724"
     CDS             complement(10527..11348)
                     /gene="cysE"
                     /locus_tag="OHN27_002724"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCQ2287133.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11428..12447)
                     /gene="gpsA"
                     /locus_tag="OHN27_002725"
     CDS             complement(11428..12447)
                     /gene="gpsA"
                     /locus_tag="OHN27_002725"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCQ2287134.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12447..12914)
                     /gene="secB"
                     /locus_tag="OHN27_002726"
     CDS             complement(12447..12914)
                     /gene="secB"
                     /locus_tag="OHN27_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCQ2287135.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12977..13228)
                     /gene="grxC"
                     /locus_tag="OHN27_002727"
     CDS             complement(12977..13228)
                     /gene="grxC"
                     /locus_tag="OHN27_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCQ2287136.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDALHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13370..13801)
                     /locus_tag="OHN27_002728"
     CDS             complement(13370..13801)
                     /locus_tag="OHN27_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCQ2287137.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14046..15590
                     /gene="gpmM"
                     /locus_tag="OHN27_002729"
                     /gene_synonym="pgmI"
     CDS             14046..15590
                     /gene="gpmM"
                     /locus_tag="OHN27_002729"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCQ2287138.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15600..16883
                     /gene="envC"
                     /locus_tag="OHN27_002730"
     CDS             15600..16883
                     /gene="envC"
                     /locus_tag="OHN27_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCQ2287139.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16887..17846
                     /locus_tag="OHN27_002731"
     CDS             16887..17846
                     /locus_tag="OHN27_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCQ2287140.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(17833..18867)
                     /gene="waaH"
                     /locus_tag="OHN27_002732"
     CDS             complement(17833..18867)
                     /gene="waaH"
                     /locus_tag="OHN27_002732"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCQ2287141.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTIALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19106..20131)
                     /gene="tdh"
                     /locus_tag="OHN27_002733"
     CDS             complement(19106..20131)
                     /gene="tdh"
                     /locus_tag="OHN27_002733"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCQ2287142.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20141..21337)
                     /gene="kbl"
                     /locus_tag="OHN27_002734"
     CDS             complement(20141..21337)
                     /gene="kbl"
                     /locus_tag="OHN27_002734"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCQ2287143.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVIEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            21551..22483
                     /gene="rfaD"
                     /locus_tag="OHN27_002735"
     CDS             21551..22483
                     /gene="rfaD"
                     /locus_tag="OHN27_002735"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCQ2287144.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22493..23539
                     /gene="rfaF"
                     /locus_tag="OHN27_002736"
     CDS             22493..23539
                     /gene="rfaF"
                     /locus_tag="OHN27_002736"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCQ2287145.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALFGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23543..24514
                     /gene="rfaC"
                     /locus_tag="OHN27_002737"
     CDS             23543..24514
                     /gene="rfaC"
                     /locus_tag="OHN27_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCQ2287146.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(24584..>25093)
                     /locus_tag="OHN27_002738"
     CDS             complement(24584..>25093)
                     /locus_tag="OHN27_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCQ2287147.1"
                     /translation="AGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGL
                     KTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYVDSHLHNDLIDTLSTRGIPGVVLTI
                     LAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFITIILLCAYFKA
                     QSDQCLLEK"
BASE COUNT         6345 a         6476 c         6413 g         5859 t
ORIGIN      
        1 gcaacataga tcaccaataa tacaatggac aactctaccg gcattgatcg ctggtcggag
       61 gcaataagga ttttcacatg aacttgaaga agattttttt cagtgctgtc actgtttcag
      121 ttctctgcgc attaacggga tgtgactaca ttcaagaagg caagccagaa agtagtttac
      181 tgaaacaaga agaagaacat aacaataaaa ttgtgctact tgaaaaacaa caagcacagc
      241 taaagagtca actcgaaact attcaaaaac aacaaactgg cataataaac agcacaaaga
      301 ctttaactca cgtaattaaa tccgttaaag accaacaaaa tacttttatt tttaccgagt
      361 ttaacccagc aaaaacgaag tatttcatcc tgaacaacgg ttcggtggct ttagcgggcc
      421 gagtgttatc cattgacgcc acagagaatg gcagtgttat tcatatttca ctggtcaact
      481 tattaagtat accaatctca aacattggct ttaatgcgac atggggtggt gaaaaacctg
      541 tcgatgccaa agagtttgcc cggtggcaac aattactttt caacacatca atgacatcca
      601 cattgaaatt attaccaggt caatggcaag acattaattt gacactgaag ggtgtatcgc
      661 caaataacct gggatatctg aagttagcca tcaatatgga aaatattcag tttgacaatc
      721 ttcccgctgc tgataatcgg cagaaaagaa gcaaaaaata aatgcaatta gtacgcttat
      781 aacaaggaat agcgagcatc attaatgaac aaaatattta aagttatctg gaaccctgcc
      841 acagggaatt atactgttac cagcgaaacg gcaaaaagcc gtggcaagaa atctgggcgc
      901 agtaagctgt taatttctgc gctggttgcg ggtgggttgt tgtcatcgtt tggggcgctg
      961 gctgataatt acgacggtca gggcgttgat tacggcgatg gctcagctag tgacggctgg
     1021 gttgctatcg gtaaaggtgc aaaagcaaat atttttttaa acaacgctgg tgccagtacc
     1081 gctttaggtt atgacgctat agctgaaggc caatatagct ctgccatcgg ctcaaaaacc
     1141 catgctattg gtggtgcatc aatggccttt ggggttagtg caatctctga aggtgacagg
     1201 agtatcgcgc tgggtgcatc gtcgtattca ttcggccaat actcaatggc ccttggtcgt
     1261 tattccaaag cgctgggtag attgtctata gctatggggg atagctccaa agcggatgga
     1321 gcaaacgcca ttgcgctggg aaatgccgct aaggcggctg gtattatgag catcggtctc
     1381 ggtgataatg ccaatgcgtc acaagattat gctatggcgc tgggagcaga aagcgaagcc
     1441 gctgaaaatg cgaccgctat cggcaataaa gcgcatgcaa aaggagtgaa tagcatcgcg
     1501 ttgggtaatg gcagccaggc tctggcagat agtgcaattg ctattggcca gggcaacaag
     1561 gctaacggcg ctgatgctat cgctctgggt aatggtagcc agtcgagtgg cttaaacgct
     1621 attgccttag gtaaggccag tgttgtaact ggcgataata gtcttgcgct tgggagcaat
     1681 accaatgcca acggtattaa ctctgtcgcg ctgggcgcag gttctattgc ggatcaagac
     1741 gatagcgttt ctgtcggcag tgactcatta caacgcaaga tcgttaatgt aaaaaatggc
     1801 acgatcaagg ctgatagcca cgacgccatt aatggctcac agctttatgc catcagcgac
     1861 tcggtggcaa aacggcttgg cgggggttca tcggtaaacg ttgacgacgg tactgttaaa
     1921 gcaccaacct acaatttaaa aaatggtaac aaaaataacg ttggcgatgc gctcactgta
     1981 ctcgatcaat tcaccctgca atgggatcaa aacagagaca aatacagcgc cgctcatggt
     2041 agctcaactg ccagcgtaat caccgatgtg gcggatggcg cagtttcaga ttccagtaaa
     2101 gacgcggtta acggttcaca actgaaagcc accaatgatg atgttgagac caataccacc
     2161 aatatcgcta ccaataccgg aaatattgca actaacactg caaatattgc taccaacacc
     2221 accaatatca ctaatttgac agatactgtt ggcgacctta aagatgatgc cctgctctgg
     2281 aatggcactg cattcaacgc cgctcatggt acggaaacca ccagcaccat caccaacgtg
     2341 aaagctggta ccctttcgga tgacagtact gacgcggtta atggctcgca actgaaagac
     2401 actaacgata acgtggcgac caacaccacc aatatcgcta gcaacacggc taacattgcc
     2461 actaacacca gtaatattgc cgataacact gccaacattg ctaccaacac cagtaatatt
     2521 gccgataaca ctgccaacat tgcgaccaac accagcaata ttgcaggtaa cactgccaac
     2581 attgctacca ataccactaa tatcgcggct aacaccacaa gcataaatag cctgaacacg
     2641 tctgtggatg ctcttgaaca ggatgctatg ctctggaacg gcactgcatt caacgccgct
     2701 catggtacgg aaaccaccag caccatcact aatgtgaaag ctggcaccct ttcggatgac
     2761 agtactgacg cggttaatgg ctcgcaacta aaagctacta acgataacgt ggcaaccaac
     2821 accaccaata tcgccagcaa tacggctaac attgcgacca acaccgccaa tattaatact
     2881 ctcaatacgt cgatcgatac ccttgagcag gatgcaatac tctggaacgg aacagcgtat
     2941 agcgccgcgc acggtactga aacagccagc actattacca acgttaaagc aggtacttta
     3001 tctgaaaata gtacggacgc agttaacggt gcgcagctga acgcgactaa cgcgaatgtg
     3061 gcgaccaaca ccaccaatat tgctactaac accgccagca ttaatactct gaatacatcc
     3121 attgacgctc tggaacagga tgcgctgctt tgggatggca ctgcattcag tgcagcgcat
     3181 ggtgcaaaca aagatgccag caaaatcacc aatgtcctgg cagggactgt atcatccgcc
     3241 agtactgatg ccattaatgg cagccagctt catgggttga gcagttcgat cgccacctat
     3301 cttggtggtg gtgccactgt gagcgattct ggcgtattta gcggccctac ctataacatt
     3361 gatggtaatg attacaccaa tgttggtgct gcacttgacg ccattaacac ctcacttagc
     3421 gactcactcg gcgatgccct cctttgggat agcacgactg gcgcatttag tgccaaacac
     3481 ggttctaccg ccagcgtaat cactaacgtc gcagacggtg cagtctctga ctctagctct
     3541 gatgctgtga atggttcaca actgtacgat gtaagcaact ctgttgtcga tgttctgggt
     3601 ggtggtgctg gcgtgaatac ggatggcagc atcagtgcac caacgtacac cattgctaac
     3661 actgattacg ataatgtcgg cgatgcccta aacgcgcttg ataccactct tgacgatgcg
     3721 atgctatggg atgctaccgc aggtgaaaat ggtgccttca gtgccagcca cgatggcagt
     3781 gccagcaaaa taactaacgt cgcggcagga acaatttctg acaccagcac cgatgcggtt
     3841 aatggggctc aactccacgg cgttagcagt tccgtcgctg aggctcttgg tggtggtgcg
     3901 gcggtgaatt ctgacggcag catcagcgca ccgacttaca ccattgctga taccgactat
     3961 accaacgtcg gcgatgcgat gaatgcaatt gactcaaccc tggataacgc cttgctttgg
     4021 gacgctgccg caggtgaaaa cggtgcgttt aacgctagcc atgatggcaa agccagtgtc
     4081 atcaccaacg tagccaatgg tcagattaac gaaaccagca ccgacgcggt taacggttcc
     4141 cagttaaatg ccacgaatat gttgatccag aatattgccg gtgataccag cgaaagctat
     4201 atcacagaga acggtgaagg tatcaactat gtgcgtacta acgacagcgg tttagtcttt
     4261 gaggatgcca gtgctacagg tgtgggcgct acagctgtag gttataactc cgtcgcatca
     4321 ggcgatagca gtgtggcaat tggacagaat agcagcagta ctattgaatc tggaattgct
     4381 ctgggcagca gttctgtgtc taaccgtgtc atccttcagg gctcccgtga taccagcgta
     4441 accgaagatg gcgtagtcat tggttataac accagtgatg gcgaactgct tggcgcgttg
     4501 tcaattggtg atgacggtaa atatcgtcaa atcatcaacg tcgccgatgg ttccgaagcc
     4561 catgacgccg ttacggttcg ccagttgcag aatgcgattg gtgctgtagc aacgacaccg
     4621 tccaaatact tccatgctaa ttcaacggaa gaagattcac tggctgtcgg tgaagactcg
     4681 ctggcaatgg gggcgaaaac tgtcgttaat ggtaatgcag gtattggtat cggtttgaac
     4741 actctggttc tggctgatgc gatcaacggt attgctatcg gcagtaacgc acgcgcaaac
     4801 catgcaaaca gtattgcaat gggtaacggt tctcagacca cccgtggtgc acagactggc
     4861 tacaccgcct acaacatgga cgcaccgcag aactctgtcg gtgaattctc tgtcggcagc
     4921 gaagacggtc aacgtcagat caccaacgtc gcggctggtt cagcggatac cgatgcggtt
     4981 aacgtaggtc agttgaaagt cactgatgag cgcgtaacgc aaaataccca gagcattact
     5041 aacctgaaca atcaggtcac taatctggat actcgcgtta ctaatatcga aaacggtatt
     5101 ggcgatatcg tcaccaccgg tagcaccaag tacttcaaga ccaataccga tggtgtagat
     5161 gccagcgcgc agggtaaaga tagcgtcgcg attggttccg gctccattgc tgccgctgac
     5221 aacagcgtcg ctctgggtac agggtctgtg gcaaccgaag aaaatacgat ctctgtaggt
     5281 tcctctacta atcaacgtcg tatcaccaac gtagctgcag gtaaaaatgc taccgatgct
     5341 gttaacgtgg cacagttgaa gtcttccgaa gctggcggtg tacgttacga caccaaagct
     5401 gatggttcta tcgactatag caatatcacc ctcggtggcg gcaacggcgg tacgactcgt
     5461 atcagcaacg tctccgctgg cgtcaacaac aacgacgcgg tgaattacgc gcagttgaag
     5521 caaagcgtgc aggaaacgaa gcaatacacc gatcagcgaa tggttgagat ggataacaaa
     5581 ctgtctaaaa ctgaaagcaa gttgagcggt ggtatcgctt ctgcaatggc aatgaccggt
     5641 ctgccgcagg cttacactcc aggtgccagc atggcctcta ttggtggcgg tacttacaac
     5701 ggtgaatcgg cagttgcttt aggtgtatcg atggtgagcg ccaatggtcg ttgggtctac
     5761 aaattacaag gtagtaccaa tagccagggt gaatactccg ccgcactcgg tgccggtatt
     5821 cagtggtaat gatccattaa gttagtgtga ctaagggcaa acgccaaatt gcctgatgcg
     5881 ttacgcgcat caggcaagat aaggcgctcc gggtcaacaa tctaagggca attctctgat
     5941 gaggattgcc cttttcttta ccagacatct ccccccacaa gaattggccc taccaattct
     6001 tcgcttatct gacctctggt tcacaattcc ccaattaaaa ctcacatcaa tattgccaat
     6061 acgtaacatt tagttaacca ttcattgtca ttatccctac acaacacaat tggcagtgcc
     6121 acttttacac aacgtgtgac aaggagatga gcaacagact cattacacga tgtgcgtgga
     6181 ctccaggaga cctgcaatga atctctggca acaaaactac gatcccgccg ggaatatctg
     6241 gctttccagt ctgatagcat cgcttcccat cctgtttttc ttctttgcgc tgattaagct
     6301 caaactgaaa ggatacgtcg ccgcctcatg gacggtagca atcgcccttg ccgtggcttt
     6361 gctgttctat aaaatgccgg tcgctaacgc gctggcctcg gtggtttatg gcttcttcta
     6421 tggtttgtgg cccatcgcgt ggatcattat tgcagcagtg ttcgtctata agatctcggt
     6481 gaaaaccggg cagtttgaca tcattcgctc gtctattctt tcgataaccc ctgaccagcg
     6541 tctgcaaatg ctgatcgtcg gtttctgttt cggcgctttc cttgaaggag ccgcaggctt
     6601 tggcgcaccg gtagcaatta ccgccgcatt gctggtcggc ctgggtttta aaccgctgta
     6661 cgccgccgga ctgtgcctga ttgttaacac cgcgccagtg gcatttggcg cgatgggcat
     6721 tccaatcctg gttgccggac aagtaacagg catcgacagt tttgagattg gccagatggt
     6781 ggggcgtcag ctaccgttta tgaccattat cgtgctgttc tggatcatgg cgattatgga
     6841 cggctggcgc ggtatcaaag agacgtggcc tgcggtcgtg gttgcgggcg gctcgtttgc
     6901 catcgctcag taccttagct ccaacttcat tgggccggag ctgccggaca ttatctcttc
     6961 gctggtatca ctgctctgcc tgacgctgtt cctcaaacgc tggcaaccag ttcgcgtatt
     7021 ccgcttcggt gacttggggg cgtcacaggt tgatatgacg ctggcccaca ccggttacac
     7081 cgcgggtcag gtgttacgtg cctggacacc gttcctgttc ctgaccgcta ccgtaacact
     7141 gtggagcatc ccgccgttta aagccctgtt cgcatcgggt ggtgcgctgt atgagtgggt
     7201 gatcaacatt ccggtgccgt acctcgataa actggttgcc cgtatgccac cagtggtcag
     7261 cgaggctaca gcctatgccg ccgtgtttaa gtttgactgg ttctctgcca ccggcaccgc
     7321 cattctgttt gctgcactgc tctcgattgt ctggctgaag atgaaaccgt ctgacgctat
     7381 cagcaccttc ggcagcacgc tgaaagaact ggctctgccc atctactcca tcggtatggt
     7441 gctggcattc gcctttattt cgaactattc cggactgtca tcaacactgg cgctggcact
     7501 ggcgcacacc ggtcatgcat tcaccttctt ctcgccgttc ctcggctggc tgggggtatt
     7561 cctgactggg tcggatacct catctaacgc cctgttcgcc gcgctgcaag ccaccgcagc
     7621 acaacaaatt ggcgtctctg atctgttgct ggttgccgcc aataccaccg gtggcgtcac
     7681 cggtaagatg atctccccgc aatctatcgc tatcgcctgt gcggcggtag gcctggtggg
     7741 caaagagtct gatttgttcc gctttactgt caaacacagc ctgatcttca cctgtatggt
     7801 gggcgtgatc accacgcttc aggcttatgt cttaacgtgg atgattcctt aatgattgtt
     7861 ttacccagac gcctgtcaga cgaggttgcc gatcgtgtgc gggcgctgat tgatgaaaaa
     7921 aacctggaag cgggcatgaa gttgcccgct gagcgccaac tggcgatgca actcggcgta
     7981 tcacgtaatt cactgcgcga ggcgctggca aaactggtga gcgaaggcgt gctgctcagt
     8041 cgacgcggcg gcgggacgtt tattcgctgg cgtcatgaca catggtcgga gcaaaacatc
     8101 gtccagccgc taaaaacgct gatggccgat gatccggatt acagtttcga tattctggaa
     8161 gcccgctacg ccattgaagc cagcaccgca tggcatgcgg caatgcgcgc cacacctggc
     8221 gacaaagaaa agattcagct ttgctttgaa gcaacgctaa gtgaagaccc ggatatcgcc
     8281 tcacaagcgg acgttcgttt tcatctggcg attgccgaag cctcacataa catcgtgctg
     8341 ctgcaaacca tgcgcggttt cttcgatgtc ctgcaatcct cagtgaagca tagccgtcag
     8401 cggatgtatc tggtgccacc ggttttttca caactgaccg aacaacatca ggctgtcatt
     8461 gacgccattt ttgctggtga tgccgacggg gcgcgtaaag caatgatggc gcaccttagt
     8521 tttgttcaca ccaccatgaa acgattcgat gaagatcagg ctcgccacgc acggattacc
     8581 cgcctgcccg gtgagcataa tgagcattcg agggagaaaa acgcatgatt atttccgcag
     8641 ccagcgatta tcgcgccgca gcgcaacgca ttctgccgcc gttcctgttc cactatatgg
     8701 atgggggtgc atattctgaa tacacgctgc gccgcaacgt ggaagatttg tcagaagtgg
     8761 cgctgcgcca gcgtattctg aaaaacatgt ccgacttaag cctggaaacg acgctgttta
     8821 atgagaaatt gtcgatgccg gtggcgctag gtccggtagg tttgtgtggc atgtatgcgc
     8881 gacgcggcga agttcaggca gccaaagcgg cggatgcgca tggcattccg tttactctct
     8941 cgacggtttc cgtttgcccg attgaagaag tcgcgccagc catcaagcgc ccaatgtggt
     9001 tccagcttta tgtactgcgc gatcgcggct ttatgcgtaa cgcgctggag cgagcaaaag
     9061 cagcgggttg ttcgacgctg gttttcaccg tggatatgcc gacaccggga gcgcgttatc
     9121 gtgatgcgca ttctggaatg agcggcccga acgcggcaat gcgccgctat ttgcaggcgg
     9181 tgacgcatcc gcaatgggcg tgggatgtgg gcctgaacgg tcgtccgcat gatttaggaa
     9241 atatttcggc ttacctcggc aaaccgaccg gactggaaga ttacatcggc tggctgggga
     9301 ataacttcga tccgtccatc tcatggaaag accttgagtg gatccgcgat ttctgggatg
     9361 gcccgatggt gatcaaaggg atcctcgatc cggaagatgc gcgcgatgca gtacgttttg
     9421 gtgctgatgg gattgtggtt tctaaccacg gtggccgcca gctggacggt gtgctctctt
     9481 ctgcccgtgc actgcctgct attgccgatg cggtgaaagg tgatatcgcc attctggcgg
     9541 atagcggaat tcgtaacggg cttgatgtcg tgcgtatgat tgcgctcggt gccgacaccg
     9601 tactgctggg tcgtgctttc ctgtatgcgc tggcaacagc gggccaggcg ggtgtagcta
     9661 acctgctaaa tctgatcgag aaagagatga aagtggcaat gacgctgact ggcgcgaaat
     9721 cgatcagtga aattacgcaa gattcgctgg tgcaggggct gggtaaagag ttgcctacgg
     9781 cactggctcc gatggcgaaa gggaatgcgg catagtcgtt tgagccccct caccctaacc
     9841 ctctccctca gggagtgggg accgttcggc gctgtatgta ctccctcact ctgaaacgac
     9901 accgcactct ttttttctcc ctcgcccctc cggggtgagg ggaaaaggcc gcactgctca
     9961 ccaattttct gctatcctgc ccccgcacta agggggcagt atgctaaaca tcgtacttta
    10021 cgaaccagaa attccgccaa atactggcaa tatcatccgt ctttgcgcta ataccggctt
    10081 tcgtctgcat atcatcgaac cgatgggatt tgcctgggac gataagcgcc tgcgccgcgc
    10141 ggggctggac tatcacgagt ttaccgccgt tacgcgtcat catgactttc gcgcgttcct
    10201 cgaagcagaa aatccccagc gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag
    10261 cgccgtaaac tatcaggatg gcgattatct gatgttcggc ccggaaacac gcggcctgcc
    10321 agcgagcatt cttgatgccc tgcccgctga acaaaaaatt cgcattccga tggtgccgga
    10381 cagccgcagc atgaacctgt ccaatgcggt gtcagttgtg gtgtatgaag cctggcggca
    10441 gctgggatat ccgggagcgt tgttgagaga ttagttactg tatgccggat agatgattac
    10501 atcgcatccg gcacgatcac aggacattag atcccatccc catactcaaa tgtatggtta
    10561 ataccgttga aatgctggtc catatccatt gatggcttat cgctgtctgg tttaccgaca
    10621 atacgagccg gaacgccagc ggcggttgta tgcggcggca ccggttgcag caccacggaa
    10681 cctgcgccaa tcttcgcgcc gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg
    10741 ccaatcatca caccttcacg aattttcggg tgacggtcac caccagattt acccgtaccg
    10801 ccaagcgtca cagattgcag aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca
    10861 acgatgcccg tggcgtggtc gagcataatg ccacggccaa tttttgctgc cgggtgaata
    10921 tcgacctgga acgtcacaga aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc
    10981 tgattccaca accagtgacc gatgcgatag gcctgcaagg catgaaaacc cttcaggtat
    11041 aacaacgggg ttgagtattt atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca
    11101 caggccgcag aggcgatcat ttccgggtca gcggcgtagg cttcttccac cacttcacgg
    11161 atagcaatag caggcataat tggcgatgac agcttgttcg ccagcatgta gctcagtgca
    11221 ctgccaaggt tttcgtgttt gagtagcgtc gcgtggtaaa aactggccag cattggctca
    11281 cagtccgcca gcgttctggc ttcggcttta atattgttcc agacaatttc cagttcttca
    11341 cacgacattg cttactccac acgatgagat aatgaccggc ccgttctgcg cgggccgggt
    11401 catagcggta acaaaggttc cctgaggtta gtggctgctg cgctcgtcct tgcgtgcacg
    11461 acctaataac gtcaatgctg cctcgcgcgc gttttttccg caatataata cttgataaat
    11521 ttcctcggtt attggcattt caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt
    11581 attgcggtag ccttccacca cctgaccaat cttctcctgc gcgctttgaa catccatgcc
    11641 ctgaccgagc atcatgccaa aacggcggtt acgcgactgg ttgtcggtac aggtaagcac
    11701 cagatccccc agccccgcca tgcccataaa ggtggcagga tcggcaccca gcgccgcgcc
    11761 aagacgcgac atttcagcca gcccacgggt gatcagcgcc gtacgcgcat tcgcaccaaa
    11821 accgataccg tcggacatcc ccgcaccaat ggcaatgacg tttttcaccg cgccgccaag
    11881 ctgcacgcca atgaaatccg gattgctgta aacgcggaaa cttttgccgc agtgcagcag
    11941 ctgctggaga tcatcggcaa aggtctgatc ggtcgaggcc agcgaaatag ctgtcggtaa
    12001 acctgccgcc agttctttcg caaacgttgg gccagagata accgccagcg gaatttgatc
    12061 gcctaacgcc tcgcgggcca cgtcctgtaa cagacgcccg gtttccgctt ccagcccttt
    12121 ggtcgcccac accagacgcg catcaggacg catcagcggt ttaatctggc gcagcacttc
    12181 accaaagaca tggctgggta cgacgacgag aatattacgg ctggctgcca gcgcagtggc
    12241 gagatcgctt tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt
    12301 acagcggtcg cgttcaagcg ttgcgatatg ttcagggtca tggccccaga ggacaacctc
    12361 gtggccattt cttgccaggg taatggcaag agcggtgccg tacgagccgg caccgatcac
    12421 agtcattgaa gcattacgtt ggttcatcag gcatcctgat gttcttcagt accttcgcca
    12481 gcctgctgct gcaaatagtt catgaacagc gcatcgaagt taaccggcgc aaggttcagt
    12541 tgcgggaatg taccgcggga taccatgctg gtgatgcact cacgagcata cgggaacaga
    12601 atgttcgggc agtatgcgcc caggcaatgt gccatctggg tgccttcgat acccgcgatg
    12661 gagaaaatac cgccctgctg aacttcacac aggaacgcgg tttcttcgcc cagggaggcc
    12721 gttacggtaa cacgcagtac cacttcgtat acgtcatctg ccagttggga agatgccgta
    12781 tccagatcaa gtttaacttc tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc
    12841 gcttcgaaag agatatcctt ggtataaata cgttggatct ggaaagtcat ttcagtgttg
    12901 ttttgttctg acatgtgtag aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa
    12961 tgccaacgtt cacacgttat ttcagcaggg gatccagtcc accacgtgca tccaatgcat
    13021 acaagtcatc acagccgcca atgtgcagtg cgtcaataaa aatctgggga accgtggtgc
    13081 gaccgctgcg tttgatcatc tcttcacgct tggcggcatt gccatcgatc ggcaactcct
    13141 gaaaactcac gcccttgctg ctcagcagtg cttttgcacg atggcaatac gggcaggttt
    13201 ctttggtata gatttcaaca ttggccataa tttagctcct gtttttttac cctgaaattt
    13261 catgttgcag ggcggctgca aaatggagtg tccccagaag cttataacaa taagtcgctg
    13321 ggggcctaat tgcagctaac ggcctgcatc atgaaagacg acaggtaaat tatttgccgc
    13381 gcaccagagg caagttttcg ccagcccagc cagcaacgcc ctctttcagc acgaatactt
    13441 gcgcaaaacc agctttcgtc agcgcgtttg caggctcctg gcactgcatg ccagaaccgt
    13501 ctaccacgat aaccggtttg tctttgtgct tctcaagctc accaacattg ttggctttga
    13561 tttcgctcgg caacagattg atagaacctg cgatatggcc tttacggaag tcatcacgct
    13621 gacgtaaatc cacaaccaca gcgtcttctt tgttgatcag acgcgtagct tcaccacgag
    13681 taatcacctt cactttcgag gtcaggctct taaacgtagt cacaagaacc gccaccagta
    13741 acgcgatcca ggcgatactc agtatgggat gacggccaac aaattgcata atttcttgca
    13801 tggggggtaa caactcccga cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc
    13861 ggtgcggcaa atacagccag cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa
    13921 aaaagcgcaa atctggcatc ggcttgccgc gagcggtcgt atattttgat cttcagaggc
    13981 tattttatcg attcagctgt agtaaaatta cgcaaatttt gactcttgag tatgaggttg
    14041 tcgcaatgtc ggtttctaaa aaacctatgg tactggtgat tctggatggc tatggctatc
    14101 gtgaagaaca gcaggataac gccattttta gtgctaaaac cccggtaatg gatgcactgt
    14161 gggccaatcg tccgcatacc ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc
    14221 gtcagatggg taactccgaa gtaggccacg ttaacctggg tgccggccgc atcgtgtatc
    14281 aggacctgac tcgtctggac gttgaaatca aagatcgcgc tttctttgct aatccagtgc
    14341 tgactggcgc ggtagataaa gcgaaaaacg caggtaaagc agtacacatt atgggtctgc
    14401 tctccgcagg cggcgtacac agccacgaag atcacatcat ggcgatggta gaactggcag
    14461 ctgaacgcgg cgcagaaaaa atctacctgc acgcattcct tgacggtcgc gacaccccgc
    14521 cgcgcagtgc tgaatcctcg ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag
    14581 gccgcgtagc gtccatcatt ggtcgttact acgcgatgga ccgcgataac cgttgggatc
    14641 gcgttgaaaa agcctatgac ctgctgactc tggcgcaggg tgagttccag gccgatactg
    14701 ccgttgctgg tttgcaggca gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga
    14761 ccgttattcg tgcagaaggt cagccagatg cggcaatgga agacggcgat gcgctgattt
    14821 tcatgaactt ccgtgctgac cgcgcgcgcg aaatcactcg tgcattcgta aacgcagatt
    14881 tcgacggctt cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg accgaatacg
    14941 ccgctgacat caaaactgcg gttgcttacc cacccgcttc cctggttaac accttcggcg
    15001 agtggatggc gaaaaacgac aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc
    15061 acgttacttt cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc
    15121 tgatcaactc accgaaagtg gctacctacg atctgcaacc ggaaatgagc tccgcagagc
    15181 tgaccgaaaa actggttgcg gcaatcaaga gcggcaaata cgacaccatt atctgtaact
    15241 atccgaacgg cgacatggta ggtcacaccg gggtgatgga agcggcggtt aaagcagttg
    15301 aagcgctgga tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac
    15361 tgctgatcac cgctgaccac ggtaacgctg aacagatgcg cgatccggca acgggtcagg
    15421 cacacacggc acacaccaac ctgccagttc cgctgattta cgttggtgat aagaacgtga
    15481 aagcggttgc aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg ctgatgggta
    15541 tggaaatccc gcaagagatg actggtaagc cgctgttcat cgtggaataa tccctcccca
    15601 tgaggggaaa ggcgattaat accatgacac gggccgtgaa accgcgcagg tttgcaatca
    15661 ggcccatcat ctacgccagc gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc
    15721 acgcggatga gcgtgaccaa ctcaaatcta ttcaggctga catcgccgca aaagagcgcg
    15781 cggtacgcca aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag
    15841 aagcgatctc tgaagccact cgcaaactgc gcgaaacgca aaacacgctc aatcaactca
    15901 ataaacagat tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc
    15961 aggagcgcag cctcgccgca caactggatg ccgcattccg tcagggcgag cataccggta
    16021 ttcagctaat tctcagcggt gaagaaagcc agcgtggaca gcgtttacag gcttatttcg
    16081 gctatctcaa ccaggcgcga caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag
    16141 tcgccatgca gcgtgctgaa ctggaagaga aacaaagcga gcaacaaacg ctgttatatg
    16201 aacagcgcgc ccaacaggcg aaactgactc aggctctgaa cgagcgtaaa aagacgctgg
    16261 cggggctgga atcttccatc cagcaaggtc agcagcagct aagtgaactg cgtgccaacg
    16321 aatcccgtct gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac
    16381 gagaagcgcg tgaggcccag gcggttcgcg accgccagaa agaagcgacg cgcaaaggca
    16441 ccacctataa accgaccgaa agcgaaaaat cgctgatgtc ccgaactggt ggcctgggcg
    16501 cgccgcgtgg tcaggcattc tggccggttc gcgggccgac gctgcatcgc tatggcgaac
    16561 agctacaggg tgaattacgc tggaaaggga tggtgattgg tgcctctgaa ggtactgaag
    16621 ttaaagcgat tgccgacggt cgggtgattc tggctgactg gctgcaaggc tacggtctgg
    16681 tggtggtggt tgagcatggt aaaggcgaca tgagtcttta cggctataat cagagcgcac
    16741 tggtgagcgt tggttcgcag gttcgcgcgg gccagccaat tgcactggtg ggcagcagtg
    16801 gcggtcaggg tcggccttca ctctatttcg aaattcgccg ccagggtcag gcggtcaatc
    16861 cacagccgtg gttgggaaga taagttttgt ttccatttcg tcgtaacgtt cttgcatttg
    16921 ccgctctgtt ggcgctctcc tcccccgtac ttgctggcaa acttgccatc gtcattgatg
    16981 attttgggta tcgcccgcac aacgaaaacc aggtgctggc gatgccttcc gctatctccg
    17041 tcgctgtatt acccgattca ccgcacgcca gagagatggc gaccaaagcg cataacagcg
    17101 gtcacgaagt gttgattcat ctcccgatgg caccgttgag taaacagccg ctggagaaaa
    17161 atacgctacg cccggagatg agcagcgacg aaattgagcg cattattcgt agtgcggtca
    17221 ataacgtgcc ctatgccgtg gggatcaaca accacatggg cagcaagatg acctctaacc
    17281 tgtttggtat gcagaaagtg atgcaggcgc tggagcgtta caatctttac ttcctcgaca
    17341 gcgtaaccat cggtaatacc caggcgatgc gcgccgcgca aggcactggc gtgaaggtga
    17401 tcaaacggaa ggtattcctc gacgattcgc aaaatgaagc ggacatccgt gtgcaattta
    17461 atcgcgcaat tgatctggcg cgtcgcaacg gttcaaccat tgccattggg catcctcacc
    17521 cttcaacggt acgcgtgcta caacagatgg tttataacct gccgccagac attacgctgg
    17581 tgaaagccag cagcttgctg aatgaaccgc aggttgatac gtctacaccg ccgaaaaaca
    17641 ctgtgcccga cacaccgcgt aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac
    17701 cgatagaacc tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc atcagccaga
    17761 gcacgctgat cgtttacttc cagcatcagt ggcaaggctg gggcaaacag cccgaagcgg
    17821 caaaacttaa cgctagcgca aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt
    17881 tttgtcgcgc cattgccata agcggaaaga ccacagtaac gcctggtaac ccaccttcac
    17941 gctgcgcaca ttggtaatca ggcgcttata cataccggaa gtaaatatct cggcaatcat
    18001 ccgttggcgg gtaagaatat ccggctcttt gcgcaccgca tggcaaacac gcaatgcttc
    18061 gtaagttatt tgctgatgaa attccggata aatcgtaatt ttgtcggcat aatttcgatt
    18121 taatttctcc agcaggcggg taatcttaat atagtgacgt tgataattaa ggtttttatt
    18181 cccttgtcta tgcaaccgac tcactgacgt attatgcaga taatatttat ataatgattg
    18241 ctcggtatat cgcgcacgca gcgcattaaa catgaattct gttgtccaga caatatcctg
    18301 atgatgtaat ccggcaataa atttaatgtt atttttaaca ataacatcgc ggcgataaac
    18361 ccccatccag acaacgtgag tccaacggcg cgaagaaagc cccatccgca gccagtccgg
    18421 gccggttaat acaccggttg agcgaaggcg atcgctgggg atggattgcc aggtttctcc
    18481 cgtttcacga aaagaccagt cagcgttgca ctgcgccacg tcgaggtcgt cctctaacgc
    18541 tatggtcatc agcgtttcgt acatggtggg atagacttca tcgtcagcat cgacaaaagc
    18601 gacatatttg cccgttgcca cttcaatccc acgattacgc gccaccgatg cgccagcatt
    18661 cgcctgatgc aacaaacgaa cgtgcggata gttttctgcg taatgctttg ctatttcaac
    18721 agaattatcc gttgaaccat cgttaataat aatgatttcc agagcagtcc aggtttgcgt
    18781 aattaaagat tccatacaag tgcggaaatc atcgcccgca ttatataacg gaataataac
    18841 actaagttta ttggtgctgt tcatcataaa ttaccgacga cctctgaagt tatcctgacg
    18901 ttttacaggc tttgtattaa gaaactatta agcctgtgag atattatttt tacaggtgag
    18961 agagtcactt cagggtagag agataatgag agcagctata taagagatca gcacaattac
    19021 gaactcttta ttacactgac aggattttac atatttaaca gattatcctc agataaaagg
    19081 gctggaatac cagcccttgt tcgtgttaat cccagctcag aataactttc ccggactggc
    19141 ccgaacgcat ggcgtcaaag cccttctgga aatcatcgat agagaaacga tgggtaatga
    19201 tcggcgagag atcgaggcca gactgaatca gcgccgccat cttgtaccag gtttcaaaca
    19261 tctcacgacc gtaaatgcct ttaatgaaca agcccttaaa gatcactttt gtccagtcta
    19321 tcgacatgtc agaaggtgga atacccagca tcgcaatacg accgccgtgg ttcatggtgt
    19381 cgagcatggt acgaaacgct ggcggcgcac cggacatttc cagaccgaca tcaaagcctt
    19441 cggtcatgcc cagttctgcc atcacatcat taaggttttc tttagcgacg ttgaccgcac
    19501 gggtgatacc cattttgcgc gccagttcga gacgatattc attaacatca gtgatcacca
    19561 cattgcgtgc accaacgtgt ttcgccaccg ccgcagccat aatacctatc ggacctgcac
    19621 cagagaccag cacatcctcg ccaaccagat cgaacgacag cgcggtatgc acggcgttac
    19681 cgaagggatc aaaaatggaa gccaggtcgt cggagatatt gtctgggatt ttgaaggcgt
    19741 tgaacgccgg gatcaccaga tattcggcga agcagcccgg acggttaacg ccgacgccga
    19801 tcgtgttgcg gcacagatgg gtacgaccgc cacgacagtt gcgacagtga ccgcaagtga
    19861 tatggccttc gccagaaacg cgatcgccaa tcttgaagcc tttcacttcc tgaccaatac
    19921 ctaccacttc accgacatat tcatggccca cgaccatcgg caccgggatg gttttttgcg
    19981 accactcatc ccagttatag atgtgaacgt cagtcccgca gatggctgtt ttacggattt
    20041 taatcagcag atcgttatga cccagttccg gtacaggaac gtcggtcatc cagatgccct
    20101 cttccgcttt cagtttggat aacgctttca tctcacatcc tcaggcaata acgcccagtt
    20161 gtttaccaat acgcgtgaat gcttctactg cacgcgtaat ttgctcaggg gtatgcgccg
    20221 cagacatctg ggtacgaata cgcgcctgac ctttcggaac gaccggatag aagaaaccgg
    20281 tgacgtaaat gccctctttt tgcagctcac gggcaaattt ctgcgccact accgcatcgc
    20341 caagcatgac cggaataatg gcatgatcgg ctcccgccag ggtaaagccc gccgccgaca
    20401 tttgctcacg gaactgacgc gcgttcgccc acagacggtc acgcagctcg ctacccgctt
    20461 caaccatctc cagcactttg atagaagcgg caacaatggc cggagccagc gagttggaga
    20521 acaggtacgg acgagaacgc tggcgcagcc actcaatcac ttctttgcgc gccgcggtat
    20581 aaccaccaga agccccgccc agcgctttac cgagcgtgcc ggtgatgatg tcgacacgcc
    20641 ccatcacatc gcagtattca tgggaaccac gaccattttc accgacaaaa ccgaccgcgt
    20701 gggagtcgtc taccatcacc agggcatcgt atttatctgc cagatcgcaa acgcccttca
    20761 ggttggcaat cacgccgtcc atcgagaaca caccatcggt ggcaatcagc acatgacgcg
    20821 caccggcttc acgcgcttct ttcagacgag cttcaagctc ctgcatatcg ttgttggcat
    20881 agcgatagcg tttagcttta cacagacgca caccatcaat aatagacgcg tggttcagtg
    20941 cgtcggagat aatggcgtct tccgcaccca gcagcgtttc aaacaggcca ccgttagcat
    21001 caaagcagga agagtagaga atcgcatctt ccatccccag gaaagccgcc agtttttgtt
    21061 caagctcttt atggctgtcc tgagtgccgc aaataaaacg caccgaagcc atgccgaaac
    21121 cgtgagaatc cattcccgcc tttgctgccg caatcagatc cggatgattc gccagcccga
    21181 gatagttgtt ggcacaaaag ttaatgacgt ggcttccatc agccacagtg atatctgctt
    21241 gctgcgcaga cgtaataatg cgctcttctt taaacaaccc ttccgcccgt gcggtttcca
    21301 gatcgttggt taactgctga taaaattctc cacgcattgc gattctccag acagggcaaa
    21361 ttccagcaca tattacccaa acttataggt cgcgacgaga tgacgcgtta acacttctgc
    21421 aaaattcagg ataaataacg tccatcaatc tcacttttgg cgaatggctg aagccaacgc
    21481 cgtgtagtga tatgatagaa gcattcgtgt ctgagattgt ctctgactcc ataattcgaa
    21541 ggttacagtt atgatcatcg ttaccggcgg cgcgggcttt atcggcagca acatcgttaa
    21601 ggccctgaat gataaaggca tcaccgatat tctggtggtg gacaacctga aagacggcac
    21661 caagtttgtg aacctggtgg atctgaatat cgcagactat atggataagg aagacttcct
    21721 gatccagatt atggctggcg aagagttcgg cgatgtcgaa gcgattttcc acgaaggcgc
    21781 gtgctcttcc accaccgagt gggacggcaa gtatatgatg gataacaact atcaatactc
    21841 caaggagctg ctgcactact gcctggagcg cgaaatcccg ttcctgtatg cctcttccgc
    21901 agccacctac ggcggacgca cctccgactt tattgaatcc cgcgagtacg aaaaaccgtt
    21961 gaacgtctac ggttactcaa aattcctgtt tgatgaatat gttcgccaaa tcctgcctga
    22021 agctaactcg cagattgttg gcttccgcta tttcaacgtt tatggaccgc gtgaaggcca
    22081 taaaggcagc atggcgagcg tcgctttcca tctcaacacc cagcttaata acggtgaatc
    22141 accgaagctg ttcgaaggta gcgagaactt caaacgtgac ttcgtctatg tgggcgacgt
    22201 ggcagatgta aacctgtggt tcctggaaaa tggcgtttcc ggtatcttca acctcggcac
    22261 cggtcgtgca gaatccttcc aggcagtagc agatgctacg ctggcttatc acaagaaagg
    22321 ccagatcgaa tacattccgt tcccggataa actgaaaggc cgctatcagg cgttcactca
    22381 ggcagatctg acaaatctgc gcgcggcggg ttacgacaaa ccgttcaaaa ccgttgctga
    22441 aggtgtaacg gaatacatgg cctggctgaa tcgcgacgca taagagctct gcatgaaaat
    22501 actggtgatc ggcccgtctt gggttggcga catgatgatg tcgcaaagtc tctatcgcac
    22561 gctccaggcg cgctatcccc aggcgataat cgatgtgatg gcaccggcat ggtgccgtcc
    22621 attattatcg cggatgccgg aagttaacga agctatccct atgcctctcg gtcacggagc
    22681 gctggaaatc ggcgaacgcc gcaaactggg tcatagcctg cgtgaaaagc gctacgaccg
    22741 cgcctacgtc ttaccaaact ccttcaaatc tgcattagtg cctttcttcg cgggtattcc
    22801 tcatcgcact ggctggcgcg gcgagatgcg ctacggttta ctcaacgatg tacgcgtgct
    22861 cgataaagaa gcctggccgc taatggtgga acgctatgtc gcgctggcct atgacaaagg
    22921 cattatgcgt accgcacaag atctgccgca gccattgtta tggccgcagt tgcaggtgag
    22981 cgaaggtgaa aaatcatata cctgtaatca attttcgctt tcatcagaac gtccgatgat
    23041 tggcttttgc ccgggtgcgg agtttggtcc ggcaaaacgc tggccacact accactatgc
    23101 ggagctggca aagcagctga ttgatgaagg ttatcaggtg gttctgtttg gctctgcgaa
    23161 agatcatgaa gcgggcaatg agattcttgc cgctttgaat accgagcagc aggcatggtg
    23221 tcggaacctg gcgggggaaa cacagcttga tcaagcggtt atcctgattg cagcctgtaa
    23281 agccattgtc actaacgatt ctggcctaat gcacgttgcg gcggcgctca atcgtccgct
    23341 ggttgccctg tttggtccga gtagcccgga cttcacaccg ccgctatccc ataaagcgcg
    23401 cgtgatccgt ctgattaccg gctatcacaa agtgcgtaaa ggtgacgctg cggagggtta
    23461 tcaccagagc ttgatcgaca ttactcccca gcgcgtactg gaagaactca acgcgctatt
    23521 gttacaagag gaagcctgac ggatgcgggt tttgatcgtt aaaacatcgt cgatgggcga
    23581 tgttctccat acgttgcccg cactcactga tgcccagcag gcaatcccag ggattaagtt
    23641 tgactgggtg gtggaagaag ggttcgcaca gattccttcc tggcacgctg ccgttgagcg
    23701 agttattcct gtggcaatac gtcgctggcg taaagcctgg ttctcggccc ccataaaagc
    23761 tgaacgcaaa gcgtttcgtg aagcgctaca agcagagaac tatgacgcag ttatcgatgc
    23821 tcaggggctg gtaaaaagcg cggcgctggt gacgcgtctg gcgcatggcg taaagcatgg
    23881 attggactgg caaaccgctc gcgaaccgtt agccagcctg ttttacaatc gtaagcatca
    23941 tattgcaaaa cagcagcacg ccgtagaacg cacccgcgaa ctgtttgcca aaagtctggg
    24001 ctatagcaaa ccgcaaaccc agggcgatta tgctattgca cagcatttcc tgacgaacct
    24061 gcctacagat gctggcgaat atgccgtatt tcttcatgcg acgacccgtg atgataaaca
    24121 ctggccggaa gaacactggc gagaattgat tggtttactg gctgattcag gaatacggat
    24181 taaacttccg tggggcgcgc cgcatgagga agaacgggcg aaacgactgg cagaaggatt
    24241 tgcttatgtt gaagtattgc cgaagatgag tctggaaggc gttgcccgcg tgctggccgg
    24301 ggctaaattt gtagtgtcag tggatacggg gttaagccat ttaacagcgg cactggatag
    24361 acccaatatc acggtttatg gacctaccga tccgggatta attggtgggt atgggaagaa
    24421 tcagatggtt tgtagggctc cggggaatga gttgtctcaa ttgacagcaa atgctgttaa
    24481 gcggttcatt gaagaaaacg ctgccatgat ttaaattaca actctttaat agataatttt
    24541 gaaataaaat cagcttcctt gctgatttta tttcacatat tcattacttc tctaataaac
    24601 attggtccga ttgtgcttta aaataagcac aaagcaatat tatggtgata aacacagcag
    24661 tcggaaccgg tttagaaaag agtattacat cacttagtcc tactaccagt agtgaaaaaa
    24721 gcaagattaa aatataaggt tctttagcag ttcttaaggc atatatgagt attgctgaaa
    24781 atgctaaaat tgttaataca actccaggaa taccacgcgt tgataacgta tctatgagat
    24841 cgttatgcaa atgagaatct acatagggta aagcgccact caatctaggc tctttttctt
    24901 ctagttcatg tattttttcc gcacgttttt ccagtgattg tcctattgga gaatatgttt
    24961 ttaagccaac ttcatacatt gccagacggg caccgactga agttctggtg ttatcatgcg
    25021 aataattaac taaatcgtta tttaagtcat tcattctcat aagcaatgta tctttaaaag
    25081 aaaatacacc tgc
//
LOCUS       DAJOSY010000065        24743 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJOSY010000065 DAJOSY010000000
VERSION     DAJOSY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.065"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..196)
                     /locus_tag="OHN27_002739"
     CDS             complement(<1..196)
                     /locus_tag="OHN27_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287148.1"
                     /translation="MDINYVVSKMGFVDDLINAGSFDSAYKNVKDILNGIDEINTIKE
                     NRIIILSNLAGNLIDIGSFSN"
     gene            complement(484..717)
                     /locus_tag="OHN27_002740"
     CDS             complement(484..717)
                     /locus_tag="OHN27_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HCQ2287149.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(728..916)
                     /locus_tag="OHN27_002741"
     CDS             complement(728..916)
                     /locus_tag="OHN27_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287150.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            complement(1070..3484)
                     /locus_tag="OHN27_002742"
     CDS             complement(1070..3484)
                     /locus_tag="OHN27_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCQ2287151.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSTLS
                     NLITLYAADNEQEQLRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQR
                     FNSDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCP
                     LAENLYQQGKDKSADGDTRTDITRMDDKELHDYLHSMNKKERRELAARLRLVKPKRRR
                     DYKQRITDHQRQQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQKDD
                     KWRNLY"
     gene            complement(3481..4338)
                     /locus_tag="OHN27_002743"
     CDS             complement(3481..4338)
                     /locus_tag="OHN27_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HCQ2287152.1"
                     /translation="MSTILKWAGNKTAIMPELKKYLPAGPRLVEPFAGSCAVMMETDY
                     PSYLVADINPDLINLYKKVAADCESFISRARVLFEIANREVAYYNIRQEFNCSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKELRTFAEKAQRATFIC
                     ASFDETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKVKRSIGVAAGEGKSATEIIAVSGPRCWMGFDYSRGVD
                     SSAVYGVRA"
     gene            complement(4335..4562)
                     /locus_tag="OHN27_002744"
     CDS             complement(4335..4562)
                     /locus_tag="OHN27_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCQ2287153.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKSPGVSRVLCIECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            complement(4562..4795)
                     /locus_tag="OHN27_002745"
     CDS             complement(4562..4795)
                     /locus_tag="OHN27_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HCQ2287154.1"
                     /translation="MRNIETLTTKTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHISSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            complement(4863..5204)
                     /locus_tag="OHN27_002746"
     CDS             complement(4863..5204)
                     /locus_tag="OHN27_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HCQ2287155.1"
                     /translation="MAINGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            complement(5322..5618)
                     /locus_tag="OHN27_002747"
     CDS             complement(5322..5618)
                     /locus_tag="OHN27_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="HCQ2287156.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWITGEDGKRWHPCHSQDELL
                     SELTTRKRRKSKCMRQKVKWFISFVTEGRVIQYLKMICSVAIRHCRAMAVTFSR"
     gene            complement(5626..6135)
                     /locus_tag="OHN27_002748"
     CDS             complement(5626..6135)
                     /locus_tag="OHN27_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HCQ2287157.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTMT
                     GLGASFGLL"
     gene            complement(6168..6389)
                     /locus_tag="OHN27_002749"
     CDS             complement(6168..6389)
                     /locus_tag="OHN27_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCQ2287158.1"
                     /translation="MTPNISITLNTPHVTIERYSELTGLSIDTINDMLADGRIPRHRL
                     RKDKKREKVMINLAALTVDALTDCNVVFN"
     gene            6485..7081
                     /locus_tag="OHN27_002750"
     CDS             6485..7081
                     /locus_tag="OHN27_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HCQ2287159.1"
                     /translation="MSTSKYPSEIKINPNKGGKAAIERLVEAYGFTTRQALADHLEVS
                     KSTLANRYLRDTFPADWIIQCALETGTSLKWLTTGQGLKQSSLTAATEELVKFRLAAG
                     KMIEDGSYVFDSSFLPANLSSPIVVQDGLVTYICDQKFSEVLDGNWLINIDGTYSIRK
                     ITRLPKGMIKITTAENSFECAFSDIEVVACIRSTIVSN"
     gene            7102..8778
                     /locus_tag="OHN27_002751"
     CDS             7102..8778
                     /locus_tag="OHN27_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCQ2287160.1"
                     /translation="MNSFAIVILLLAFLAPVLAVILFKQSKKHKAAIDNLTANNIALS
                     NQLIENQEKLAQTVRELSELEGRAAPLWQYEELHSAVMDAENKIKNADSIAKQKIDEA
                     QIKAAKTVNEANYQAQMTISNANSEAIAITKDARDARLKAKERLDNANNKANELISNA
                     NDNAVKIISDAEERAKEIAGSAYEAKEFAEKYEAVAKSMKNKIEGYGDEWIIPNRSVL
                     DELAENYEFTDAGKELQKARELTKSLIKTNKAASCDYVEQNRRNTAINFVLDAFNGRV
                     DSILSKVKHNNFGKLSQEIKDAFQLVNYNGSAFRSAKISDIYLQARLNELKWGVAVNE
                     IMLEEKEEQRRIKEQLREEERARREYEKAIKEAEKEEKAIQQAINKATKELMLANEEQ
                     RLALEQKIAELQLKYEEAEAKNQRAISMAQQTRSGHVYIISNIGSFGEDVYKIGMTRR
                     LEPLDRVRELGDASVPFSFDVHAMIYSDDAPSLENHLHKVFNEKQVNKINSRKEFFNV
                     NLKEIKSVIEDMNINAHWTMFAEAKEYRESLAIEQERKAATSANDELHVA"
     gene            8861..9913
                     /locus_tag="OHN27_002752"
     CDS             8861..9913
                     /locus_tag="OHN27_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2287161.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETEAKPWLGESVDRRTLKDVVELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAVNLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDCYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TLPSGDKVAAAVGITP"
     gene            complement(10018..10554)
                     /gene="rcdA"
                     /locus_tag="OHN27_002753"
     CDS             complement(10018..10554)
                     /gene="rcdA"
                     /locus_tag="OHN27_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCQ2287162.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATFAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            10638..11846
                     /locus_tag="OHN27_002754"
     CDS             10638..11846
                     /locus_tag="OHN27_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2287163.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            11846..12661
                     /gene="ybjI"
                     /locus_tag="OHN27_002755"
     CDS             11846..12661
                     /gene="ybjI"
                     /locus_tag="OHN27_002755"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCQ2287164.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            12747..13031
                     /locus_tag="OHN27_002756"
     CDS             12747..13031
                     /locus_tag="OHN27_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287165.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(13072..14304)
                     /gene="mdfA"
                     /locus_tag="OHN27_002757"
     CDS             complement(13072..14304)
                     /gene="mdfA"
                     /locus_tag="OHN27_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCQ2287166.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            14589..15185
                     /gene="ybjG"
                     /locus_tag="OHN27_002758"
     CDS             14589..15185
                     /gene="ybjG"
                     /locus_tag="OHN27_002758"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCQ2287167.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAVV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            15243..16001
                     /gene="deoR"
                     /locus_tag="OHN27_002759"
     CDS             15243..16001
                     /gene="deoR"
                     /locus_tag="OHN27_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCQ2287168.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(16048..17250)
                     /gene="dacC"
                     /locus_tag="OHN27_002760"
     CDS             complement(16048..17250)
                     /gene="dacC"
                     /locus_tag="OHN27_002760"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCQ2287169.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRVWDFVMMKFH
                     QWFGSWFS"
     gene            17497..18123
                     /gene="gstB"
                     /locus_tag="OHN27_002761"
     CDS             17497..18123
                     /gene="gstB"
                     /locus_tag="OHN27_002761"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCQ2287170.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(18120..19235)
                     /gene="yliI"
                     /locus_tag="OHN27_002762"
                     /pseudo
     CDS             complement(18120..19235)
                     /gene="yliI"
                     /locus_tag="OHN27_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(19346..19729)
                     /gene="bssR"
                     /locus_tag="OHN27_002763"
     CDS             complement(19346..19729)
                     /gene="bssR"
                     /locus_tag="OHN27_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCQ2287171.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            19942..21267
                     /gene="rimO"
                     /locus_tag="OHN27_002764"
     CDS             19942..21267
                     /gene="rimO"
                     /locus_tag="OHN27_002764"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCQ2287172.1"
                     /translation="MRKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(21314..22642)
                     /locus_tag="OHN27_002765"
     CDS             complement(21314..22642)
                     /locus_tag="OHN27_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCQ2287173.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(22650..24734)
                     /locus_tag="OHN27_002766"
     CDS             complement(22650..24734)
                     /locus_tag="OHN27_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCQ2287174.1"
                     /translation="MEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVD
                     ESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLA
                     VDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGNYNII
                     DSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYFMTT
                     RVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERS
                     NKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRK
                     LCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKS
                     AICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAM
                     EIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQ
                     IIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCV
                     EITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFT
                     GDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPF
                     SAWDKSGKLVKE"
BASE COUNT         6590 a         6098 c         5644 g         6411 t
ORIGIN      
        1 cattactaaa acttccgata tcaataagat ttccggccaa attagataat ataattattc
       61 tattttcttt gattgtattt atctcatcga taccgttaag aatatccttt acatttttat
      121 aggcagaatc aaatgaacct gcattaataa gatcatctac gaaccccatt tttgaaacaa
      181 catagttgat atccattcaa tcctctttaa actaagctaa taataccaat cattatatgg
      241 atgagatcaa tatagaacat gccatctccg gcaaaaatcg tactacatca gaccagtgcc
      301 cttcaaataa tataaattga acttagatac ttctgcttca accagtgtgt taaatccatc
      361 gcttgaactc agaacaaaaa gcggacgatc acttatcaaa atggccgtcc acctaacgcc
      421 tcgtttcact cgttgcccaa aatagtcccc catcagaatg aatcctcttg ggggcaacgt
      481 ttcttaatgc agccagctgt cgtcttccca caccttctgc ataattttca tcacttgctt
      541 cctttcttcg tccagttgca gtccggtcag ttccacaccg ttagagctac ctttgcgaat
      601 gcgaattacc gttttgggat acagggggcg cagattgcgg taaagctcgg attcaagggc
      661 gtccagggta gactggctaa tcttctgctc tttatcgatc attatttcaa tgcgcataaa
      721 agtcacctca gctgatgaca tccattgagc ggttgtattc gtgggttctg atttttgcca
      781 tgagttcatc tgtcagttca gaaacccact gcagggccag ccccttctct tcatcactac
      841 actcactagc cgctacaagc ttaagaaaaa aatcaatgcg ctggagcttc aaagactcca
      901 aaaaatagtc ctgcatcttt cctcctatga caccacaagc aatactgtat gcataaccac
      961 tgtttatatt tacagtatat aataatctta ctgatgtaaa acgttttttt acgttcatca
     1021 gcctgatata cctggtatta ttaagagcac gaattgttaa cccgcgtaat taatacaggt
     1081 tccgccactt atcatctttc tgcaaacgct ggttccgata gaaaatacgc aggcctgctc
     1141 ctgacggaat gctgccgccg cgaaggagta aatcgacctc tttctcgctg ccatcaaatc
     1201 ctctggactt cagttcatag acgagctgct gtcgctgatg atctgtaatt cgctgtttgt
     1261 agtctctacg ccgtttcggt ttcaccaggc gtaaccttgc agccagttcc cgacgctctt
     1321 ttttgttcat actgtgcagg taatcgtgca attccttgtc atccatgcgg gtgatatccg
     1381 ttctggtatc cccatcagct gatttgtctt tcccttgttg gtacaaattt tcagcaaggg
     1441 gacagttatt gccacgagtc caaggggcgc aagcgccctg gtcggctgcc gcctcctgaa
     1501 cgtcaacggc tttacgaacc attttccact tcactgcatg agtgcagatc ttgccctctg
     1561 caatgggtga ccagatgcca taaatacgaa tgccgtgatc gccataggcg gtcggctctt
     1621 cgttgatttc ataagcggtt ctgatgaggt gatatttacg gggaaccagt acgccgccct
     1681 gcttcatgat gtaggtggca aaacaaccag catcagcagc agccagaatg gcatcaagac
     1741 gcgggttatc cagtaccgga gcacctgctt ttttgtcacc ctgttgcctt gccgcctgac
     1801 cagccagcaa gcgaagttca cggtaagcct gacgccccgg aataccaaag aagcggaatt
     1861 gctgaacacg atgcagagac gcccaggcat tcacgtattc agcgttatca cgcagagatt
     1921 tacccgtttc cttgctgatc tcgccagcca gaccacgacc gtcaatgttc ttactgatat
     1981 atttcgcgat gtagcttgtc ggcgttcctt tgcgcgggtt aatcaactca gacttaaagc
     2041 gcggcccagt gttattgccc agctcctcgc ggtcttcacg gatggcaaac ttacgcagta
     2101 atgcagtgat ggcacggcgg tcttttttgc gcatgaaaca caacaggtgc cagtgaactg
     2161 tgccatcatg atgcggctca gccacccgca cgccatacca gcgcaacccg gctttgtgca
     2221 tcgccttacg aaatgcagca aacatgccga ccagataatc gctgctttgt cttaccgtcg
     2281 cgtttgtcca ggtcgggttt ggtctgccgt tatttagcgt ggaatggaaa cgcgacggac
     2341 aggtgatagt gtagaaaacg gcgcagtcac cgcgcatttc cgcgataagc tccagacctt
     2401 taacacaggc catcatctca ttgcggcgat gcgcagggtt gctgctgctg gcgtttacca
     2461 catcctccat gtccagcgtg tcgccgtctt cgttcaccag ttcatgagaa cggaaaaact
     2521 ccagcgactt acggcgctgc tcacgtttat gcatcacggc ttcatagctg acataaggag
     2581 atgctttttt gctgaccagg caaacagcgc gcaactgctc ttcccgccat tcgcaacgca
     2641 tcttccataa tttccgatac caccagtcgg cgcacaacat acgcgccagc gaacccggaa
     2701 tgagttcata cggcacgggt ttacggcggt ttcttttccg acggagttgc tcaaacgcag
     2761 gtgggatgac atccagacgc agggtttccg ctgccacctt ttcccatgtc ttgcgtattt
     2821 cttctggctt aacgtcatcg gtggcataca aatcaccaca agctgcatca aggcacatac
     2881 tcatatgcgc agctaccagg gtggacaggc gtttcacctg atcctgactc atttcaggca
     2941 ggatcagcag gccgtccagc ccttcatggc ttgccataaa gcgaaaagat gcagatagct
     3001 gactgtcgcg tacatgctcc agtcgttcca gacatggctt aatcgtctca cgtaaatagc
     3061 gggaataagc ctttggcctg cccaggctgc tgaagtattc aatacgttgc atcagcggct
     3121 tgctgatatg ggaaggctgg gcgttgacgt ccgccagaat gaccatgtct gaattaaaac
     3181 gctgctgctc atgcgccagc tttgcccggc taatgagctt atcctgctcc atttcgcgct
     3241 ggacaggatc acgtgattca ttaaagaaat aacgctccca gacctgatca ctcagtgcct
     3301 cgcggcgcaa ctgttcctgc tcgttatcgg cagcgtacag agtgatcagg tttgaaagcg
     3361 tagaaaccgg cgcaacttcc gccgggtcca gataagggtt aatggccttt ttcgggctgt
     3421 tccatgagaa tgctgcggca gcctcgttaa agccgcagca gttgttcata tcggcatgac
     3481 tcatgcacgt actccgtaca cggcagaact atccacgcca cgcgaataat caaatcccat
     3541 ccagcagcgc ggcccggaaa cagcaatgat ttctgttgct gatttaccct cgccagctgc
     3601 cacaccgatg ctgcgtttta ccttgatata gtggtgagta aaattgcgat acagcgaacg
     3661 gatcagggat gtgtcactgt tagaaacaat gaccggatgt ccttctgatg accgatgttc
     3721 aagaacggat gccaggtgat actggtcatc ttcagtgaaa ccatcagtgt gatagccgga
     3781 aaacgtaccg tcatatggcg gatcgcaata caccacatct cccgccttca acatcgccag
     3841 cgtttcatca aagctggcgc agataaacgt tgctcgctgg gctttttctg caaatgtgcg
     3901 aagttctttt tcagggaaat acggattttt ataattaccg tagggaatgt tgaaatgccc
     3961 gctcttgtta tagcgacata aaccacggta accgtgacga ttgagataca ggaaatatac
     4021 cgctttcatg aaatcagtaa tttcagttga gcagttaaac tcctgcctta tgttgtaata
     4081 agccacctcc ctgtttgcga tctcaaataa aactctggcg cgagatataa acgattcaca
     4141 atcagcggca acctttttat agaggttgat taaatcagga ttaatatccg caaccagata
     4201 gctgggataa tccgtttcca tcatcacagc acaggaaccc gcgaaaggtt caaccagtcg
     4261 cgggccagca ggaaggtatt ttttcagttc tggcataatg gcggttttat ttcccgccca
     4321 tttcaggatg gtgctcatac agcacctccg ttgtaatgtt tgcctttcag ttctgcgatt
     4381 tcctgacagg taatgcaaag ctgcactccc ggaatggcgc ggcgtcgtgc tggcggaatt
     4441 ggcgcttcac attcaatgca aagtacgcgt gacacgcccg gtgatttggc acgggctgca
     4501 cggatatggc gctggcgttc ttcttcaacg cgctgctgta cgagatccat tgcatcagcc
     4561 attagtggat ctcctgcgct tcgttctgga ttgcttcagc agtcacgcgc agcagttctg
     4621 ccgcttccac gtggtttagc tggcgggatg agatatgaca cgccaggcta tcaaggcgag
     4681 cagccattgc ttcagccctt gcccggcgtt cttccagacg agcctctgtc agtaaaatat
     4741 taagccctgc gtcatccggt ccggttttag tcgtgagggt ttcaatatta cgcataatca
     4801 attctcctga atttagataa agggatgccc ggcgggttta cgccattaat ttcattagtt
     4861 ggttaattcg gcatggttag ccgtctggga aataagctca ccactgcacg aaaatgattc
     4921 attgctttaa tcagctcccg cttttcgtca gtggtcagct cattaatgct gatgctatga
     4981 cgttcagctg gaatttttgc cataaagaat atggcagcca gtgcccgttt attttgttca
     5041 ttattgatat cccgtggatc acgcatatct ttaataaacc gctcaagctc tgactcaata
     5101 ttcaggccaa aaactttcgc ccttaactcc gcaatgtgat taagtccatt caggcgttca
     5161 ccggggctta atggaacagt tgctgcagcg ccattaattg ccatacttca tatcccccaa
     5221 acgcagctat cgttctttgt tcttacggta acgctcaaga ggagatacat tttttcgtat
     5281 cgtctcttta acctgctctc cccgtaaaaa cgtcccatcc tttaacgtga aaaagtaact
     5341 gccatcgccc gacaatgacg gatagcaaca gagcaaatca tcttcaggta ctgaataact
     5401 ctcccctctg taacgaaact gataaaccac ttcactttct gccgcataca tttggacttt
     5461 ctccgtttcc tcgtggtcaa ttcagacagc aattcatctt gtgaatgaca tggatgccag
     5521 cgttttccat cctcacccgt gatccagccg tgaccgtagt gcattgccgg gctttgtttt
     5581 accagcagcg atgcaaatga tggttctttc gtcagcataa acacctcaca gcaaaccgaa
     5641 tgaagcacca aggccagtca tggtatcaac tgcactcgcc atcgcaggat tagcctgtaa
     5701 acgggcttgc aatgaaacag cagccagagc catcagtcgt gttacagagt taatgctgct
     5761 gatagcatca cgacgaccgg cactagtttt tacatcacca gataccgcac ctgctgcaac
     5821 acgcccgatc tctgcagttg cgctcatgac gtaatgcggc agtttctctt ttgctacctc
     5881 attaatcggt acacatggca gacaatgaat ctgtgccaga aaaccatcta ccaacgttga
     5941 atcttcagtc agatcggtaa gcagccagat ttctggtgcg gttaataaat gaggttgagc
     6001 tgggttcagc ttgttccgca gaatctgcac attcatgccc gcacgttctg ccagttgcac
     6061 cagattgtgg cgcagtgcga atgcacgaca ggcttcatca aaatgtggat gtttggaaac
     6121 ttggtaatca aacatggtcg acacctctga tgtatcccaa aatggaacta gttgaataca
     6181 acattgcaat cagtaagtgc atcaacggta agagcagcaa ggttgatcat taccttttct
     6241 cttttcttgt ctttacgaag acgatgccga ggaatgcgac catccgccaa catatcgttg
     6301 attgtgtcga ttgaaagacc agtaagttcg ctataacgct cgattgtgac gtgtggcgta
     6361 ttcagagtta ttgaaatgtt aggggtcatg atgcaacatc tcctattggc ttgtggtgag
     6421 cctgtagtaa tcgtgacaag tacccaattg ggtacaaaac tgatactagg atcgcaaaag
     6481 agatatgtca acatcaaagt acccaagtga gatcaaaata aatcccaata aaggtggtaa
     6541 ggctgcgatt gagcgattag tcgaagccta tggctttacg acacgacagg ctttagctga
     6601 tcacttggaa gtatcaaaaa gcacactagc gaacaggtat ttgcgggata catttcctgc
     6661 tgactggata atccaatgtg ctcttgaaac aggaacctcc ctcaaatggt taactaccgg
     6721 acaaggcctt aagcaaagct cgctgacagc agctacagaa gagcttgtta agtttcgcct
     6781 tgccgcaggc aaaatgattg aagacggttc atatgtcttc gattcatcat ttcttcctgc
     6841 aaacctttca tcaccaattg ttgttcagga tggactcgtc acatacattt gtgatcaaaa
     6901 attttctgaa gtacttgatg gaaactggtt aatcaatatc gacggaacct attccattcg
     6961 aaaaatcaca aggcttccta aaggaatgat taaaattaca acagcagaga atagctttga
     7021 atgtgcattt tctgatattg aagtggttgc ttgtataaga agtacaatag tctcgaatta
     7081 atatagtaaa aggattttaa aatgaattca tttgccatcg ttatactctt attagcattt
     7141 ctcgcccctg ttctagctgt aatattattt aaacaaagta aaaaacacaa agcggctata
     7201 gataacctga cagctaacaa catagccctt tctaaccaac tgattgaaaa tcaagaaaaa
     7261 ctagcacaga ccgtacgaga attatcagag cttgaagggc gagcagcacc attgtggcaa
     7321 tacgaagaat tgcacagcgc agtaatggat gcagagaata agataaaaaa tgcggactca
     7381 atagccaagc aaaaaataga tgaagcccaa attaaggcgg ctaaaacagt aaacgaagca
     7441 aattatcaag ctcagatgac aataagcaac gctaatagcg aagctatagc aatcaccaaa
     7501 gacgctcgcg atgcacgcct gaaagccaaa gaacgtcttg ataatgctaa taataaagca
     7561 aatgagctaa tctcaaatgc taatgacaat gcagtaaaaa ttatttctga tgcagaagaa
     7621 agagcaaagg agattgctgg ttcagcatat gaagctaaag agtttgcaga aaaatatgaa
     7681 gcagttgcaa aatcaatgaa aaataaaatt gaaggttatg gtgatgaatg gatcatccct
     7741 aaccgtagtg tacttgatga gctggcagaa aattatgaat ttacagatgc aggcaaggaa
     7801 ttacaaaaag ccagagagtt aaccaaatca ttaataaaaa ctaataaagc tgcttcgtgt
     7861 gattatgttg agcaaaacag gcgtaatact gccataaact ttgttttgga tgccttcaat
     7921 ggaagagttg acagtatttt atcaaaagtc aaacacaaca attttggaaa actttcccaa
     7981 gaaataaaag atgcatttca actagtaaat tacaatggct ctgcctttag gtccgcaaaa
     8041 ataagtgaca tctatcttca ggcaagactc aacgagctaa aatggggtgt agcagttaat
     8101 gaaattatgc tcgaagaaaa agaagagcaa agaaggatta aagaacagct tcgtgaagag
     8161 gaaagagctc gtagagaata tgaaaaagcg ataaaagaag ctgaaaaaga agaaaaagct
     8221 attcagcaag ctataaataa agcaacgaaa gaacttatgc ttgcaaatga agaacaacgc
     8281 ttagctttag agcaaaaaat agctgaacta cagttaaaat atgaagaagc tgaagctaaa
     8341 aatcaacgag ctatttctat ggctcaacaa accagatcag gccacgtcta tatcattagt
     8401 aatattggtt catttggcga agatgtatat aaaattggaa tgacacgccg ccttgaacca
     8461 cttgatcgtg ttcgtgaact cggagatgct agtgttcctt tttcgttcga tgttcatgcg
     8521 atgatttata gtgatgatgc accatcatta gaaaaccatc tgcataaagt tttcaacgaa
     8581 aagcaggtca ataaaattaa ctcacgaaaa gagtttttta acgtaaattt aaaagaaatc
     8641 aaatcagtta ttgaagacat gaacatcaac gcccactgga caatgtttgc agaggcgaaa
     8701 gaatatagag agtctctagc tattgagcag gagcgcaaag cagccacttc cgccaacgat
     8761 gaactacatg ttgcttagca atgtatgttt catagtaatc acacattgat tactggttac
     8821 atatacagtt aaatttagcc ctctgatatg agggcttttt atggcagtac gaaaactaac
     8881 cacagggaaa tggctttgcg aatgttaccc cgccggacga agtgggcgtc gtgtgcgtaa
     8941 acaattcgcc accaaaggcg aagcactggc ttttgagcgc cacacgatgg aagaaaccga
     9001 agcaaagccc tggctgggtg aatcagtgga tcgtcgaaca ctgaaagacg tggttgagct
     9061 atggttcaaa ctacatggta aatctctgac agctgggcag catgtctatg acaaattgct
     9121 gttgatggtt gacgctctgg gcaatcctct tgcaaccgat ctcacctcta aaatgtttgc
     9181 ccactatcga gataaacgcc tgacaggcga gatctacttc agcgagaaat ggaagaaagg
     9241 agcaagcccg gtcaccatta acctggagca aagctatcta agtagtgttt ttagcgaact
     9301 atcccgtctg ggcgaatggt cgtatccgaa cccactggag aacatgcgaa aattcaccat
     9361 cgcagaaaaa gagatggcat ggcttaccca tgagcagatt gttgaattgc tggctgattg
     9421 caaacgtcag gacccaattc tggcactggt agttaagata tgcttaagca caggcgcacg
     9481 ctggcgtgaa gccgtaaatc ttacccgctc acaggtgacc aaataccgaa ttacctttgt
     9541 cagaacgaag gggaagaaaa acagaagcat ccctatcagt aaagagcttt acgaagagat
     9601 catggcgctt gatgggttca atttcttcac agactgctat tttcaatttt tatccgtgat
     9661 ggaaaaaacg tctatcgtgc tccctcgcgg tcaactcaca cacgttctgc gccatacgtt
     9721 tgcggcgcac ttcatgatgt cgggtggaaa cattctggcc ttacaaaaaa ttctcggaca
     9781 ccacgatata aaaatgacta tgcgttacgc acatctggca ccggatcatc tagaaacggc
     9841 gctccgtttc aatcctctgg caacgctgcc aagtggcgac aaagtggcgg cagcggttgg
     9901 cattaccccg taataaccac cactgaccac caacttaact tattgttttt aacctaactt
     9961 attgttttca ttaacccgtt tacataaatg ggttttttgt tatctaaaat ttatctacta
    10021 ccctgcaacc ctctcaacca tcctcaaaat ctcctcgcgc gatagcggct tacggtcggt
    10081 gacaaaatgc agcgtcatcc cctcaataaa cgcatcaagc gcgcgggcgg ttccgggttc
    10141 aaaccattgt tcgagcgttt gctgactgcg ctgcatccag ttttgcatta ccgtttttaa
    10201 tagcggtttt cggctagcca gcgcgtagag ctggtacatc agctccatat tatccggcgt
    10261 tgcaacctgt gagctgtaga tcatatcggt gatagcctgg catgcgcctt gagcatcact
    10321 aacatcgcta aaaaatgcct gatattgccg ggacataatc tcagtaaaac tgctgaacgc
    10381 ctccagcaac aactcatcaa ttcctgaaaa atagtaggtc atcgatccca acggtacccc
    10441 ggcaaaggta gcaattttgc ggtgcgtaac agcatgtatt ccgtaaagtt tcaccgcctc
    10501 cagcgtggcc tggataattt tttctcgccg ttgcggatcg ttagcgcgac gcatgtcatt
    10561 tcctctgtct gcaattgtgt acaaatgtac acaaccttgc taatgttgtc ttccctcttc
    10621 ttattttgac gtgatctatg accgtaaatt cttcacgtaa tgcattgaaa cgccgaacct
    10681 gggcgctgtt tatgttcttc tttttgccag gcctgttaat ggcgtcctgg gcaacccgta
    10741 cgcctgctat ccgcgatatt ctctctgtct cgatcgctga aatgggcggt gttctctttg
    10801 gtctgtcgat cggttcgatg agcggtattc tctgctcggc gtggttagtg aaacgctttg
    10861 ggacacgtaa tgtcatcctg gtcacgatgt cctgcgcatt gatcgggatg atgatattaa
    10921 gtctggcact ctggctgaca tcgcccctgc tctttgccgt tggtctcggc gtctttgggg
    10981 caagttttgg ttctgcggaa gtggcgataa acgttgaagg tgccgccgtt gagcgagaaa
    11041 tgaataaaac ggttttgccg atgatgcacg gtttttatag cctgggcacg ctggcaggcg
    11101 ctggtgtcgg gatggcactg acggcctttg gcgttccggc aacggtgcac attttattgg
    11161 cggcgctggt aggtatcgcg cctatttata tcgccattca ggcaatccct gacggtacgg
    11221 gcaaaaatgc tgccgatggc acccagcatg gcgaaaaagg cgtacctttt tatcgcgata
    11281 tccagttgct gctgataggt gttgtggtgc tggcgatggc ctttgccgaa ggttctgcca
    11341 acgactggtt acccttatta atggttgacg gtcacggttt tagccctact tccggctcgc
    11401 tgatttatgc cggttttacc ctggggatga ccgttggacg cttcaccggc ggttggttca
    11461 tcgaccgtta cagtcgcgtt gccgtggttc gggccagtgc actaatgggg gcgttgggta
    11521 ttgggctgat tatttttgtc gatagcgcct gggtcgctgg ggtgtctgtt gtactctggg
    11581 gactgggtgc ctcgctgggc ttcccgctga ccatttctgc cgccagcgat accggccccg
    11641 atgcaccaac ccgcgtcagt gtggtagcaa cgaccggtta tctggctttc ctcgtcgggc
    11701 cgccgctgct gggctatctc ggcgaacatt atggattacg tagtgcaatg ctggttgtac
    11761 tggcgctggt tattctcgcg gctattgtcg cgaaagccgt cgccaaaccc gataccaaaa
    11821 cgcagacggc gatggagaat agttgatgag cattaaatta attgcggtag acatggatgg
    11881 tactttctta agcgatcaaa aaacctataa ccgtgagcgg tttatggctc agtatcagca
    11941 aatgaaagca caaggaattc gctttgtagt cgccagcggg aatcaatatt atcagttgat
    12001 ctctttcttc cctgaaattg ctaatgaaat tgcctttgtg gctgaaaacg gcggctgggt
    12061 agtgagcgaa ggcaaagatg tttttaatgg cgagctgtcg aaggatgcgt ttgctactgt
    12121 cgtggaacat ttgctgacgc gcccggaagt ggaaattatt gcctgcggaa aaaatagtgc
    12181 ctatacactc aaaaaatatg acgatgccat gaaaacggtg gcggaaatgt attatcaccg
    12241 tctggaatac gtcgataact ttgacaactt agaggatatc ttctttaagt ttggcctgaa
    12301 tctttccgat gaactgattc cacaagtaca aaaagcatta catgaggcca tcggcgatat
    12361 tatggtgccg gtccacaccg gcaacggcag catcgatctg attatccccg gcgtacataa
    12421 agccaatggc cttcgccaac tgcagaaatt atggggaata gacgacagcg aagtggtggt
    12481 ctttggcgat ggcggtaacg atattgagat gctgcgtcag gcaggcttta gttttgcaat
    12541 ggaaaatgcc ggtagcgcgg tcgtcgcagc ggcaaaatac cgcgcaggct ccaataaccg
    12601 tgaaggcgta ctggatgtga tcgataaagt tcttaaacac gaagcgccat ttgaccaata
    12661 aaaacggcag cacgcccggc aacacaatgc ctgaatgaag gctgcataat tcgcggaaat
    12721 taacccgctg cattatttca ggcgaaatga ttatgaaaaa ttgtctactg ttgggcgcgc
    12781 ttttaatggg ctttactggt gtggcgatgg cgcaaagtgt caccgtcgat gtgccgagtg
    12841 gatacaaagt ggttgtggtt cctgattcag tcagcgttcc acaagcggtc agcgtcgcga
    12901 ctgtgccgca aaccgtctat gtggctcctg cgccagctcc ggcttaccgc ccgcatcctt
    12961 atgttcgcca tcttgccagc gtaggtgaag ggatggttat cgaacaccag attgacgacc
    13021 atcaccatta attcgagaat gcctgatcgc acaaagcagt caggcatttt tttacccttc
    13081 gtgagaattt cccatctgtt tatcttttaa aaagataacc atcagcgaca gccacaaaat
    13141 tccgttgaca aggttgaaga gattaaacag tccgttgccc ccgttcagcc aggcatgttt
    13201 gctgatttca ataccaacgg taaagatcag catttgcagc attcccatcg cggcagaaac
    13261 cgtaccttta ctcatatcgc tggcaaacag ggttaatcgc accagtcccg cattcgccag
    13321 accaataccg aaagcataaa tacttaaccc ggcagtcatc cataaatacg cgtgcgatga
    13381 gataaccgtt gccgcagcag cgaccaatag accaatcata atcggccagc cgcccataat
    13441 aatcagcgaa cgtacggtgc ggcgcgaggt cagacgcgct aacagcaagt tacccgcaat
    13501 taacgccccg aaaataggca cttgcagcaa gccatattca tagctgctca actgctcgcc
    13561 ggtaatgatg ataatcggcg actgggcgat ccacgccagc aacggcagac taacgaatcc
    13621 cagcgccagc gcccccgcca caaagcggcc gttcttcagc accagcttat agtcacgacc
    13681 gagttctttc agtgacagtt tctcgcctat acgcgtggcg gtttcaggca tggctcgttg
    13741 cagaccgaaa aaggagatcg ctgccaatgc ggcaaacaaa acaaacatcc cctcccaggg
    13801 cagcacatgg atccacgccg cgcccaccag cggaccaagt agcggagcaa tcagcgccac
    13861 gttcgccatc agcgcggtga tcttgataca aaccgcctct tcgaaggatt cctgaattgc
    13921 ggcgtatccc acagcgccaa tgaaacagag gcttatgccc tgcaagaagc gcaacagggt
    13981 gaattgttca atgttttgcg ccagcaatat tgccagacag gtgacgataa accacaccac
    14041 tcccgccagc atcaccggac ggcgaccaat acgatccgac agcggcccca gcagccattg
    14101 taaaaacatc ccgcccgcca gatacgcggt catcgaagta ggaacccaat caatgcccgc
    14161 ctgatattgt tccaccacgg ccaacatacc gggttgaatc atatcgttgc cgatataggt
    14221 tgaaaattcg taaagcacca gacagagagg gaaaagtaac gcctgacgtc caagcctggc
    14281 accggaagct aatttatttt gcatgcaatt tcttcgccaa taataatcgc gcagagttta
    14341 ataaaagcgc agctaacgag aaagcgaatt ttgtagctga aaccacggtt aagcacattc
    14401 ttacattatt gcgagtatag ctacgctttc tttaagtttt atttaaccta tgcccgttac
    14461 aatcacccac cgtaaacagg ccgcttgagg gaaataagac gatgccgctt tacccagttt
    14521 aacctgcact ttattctcaa cgacttgcct gtattggctc ccttttaatc actttgcgtc
    14581 gggaagttat gctggaaaat ttgaatctct ctctattctc tcttattaac gcgacgccag
    14641 actcggctcc gtggatgatc tcgttggcga tttttattgc taaagatttg attaccgtgg
    14701 tgccgttgct ggccgtggta ctttggttgt gggggcttac agcacaacgg caactggtga
    14761 taaaaatcgc tatcgcgctg gcggtcagcc tgtttgtgtc ctggacgatg ggacatcttt
    14821 ttccgcacga ccgacccttt gtcgaaaata tcggctataa cttcctgcat catgcggcgg
    14881 atgactcatt cccaagcgat cacggtacgg tgattttcac ctttgcactg gcatttttat
    14941 gctggcatcg cctgtggtcc ggctcacttt taatggtgct ggccgtcgtc attgcctggt
    15001 cgcgcgttta tcttggcgtc cactggccgc tggatatgct cggtggattg ctggcaggta
    15061 tgattggctg ccttagtgcc cagattatct ggcaagcgat ggggcataaa ctctatcaac
    15121 gtctgcaatc gtggtatcgc gtctgttttg cattaccgat ccgcaaaggc tgggtgcgtg
    15181 actgatctct gctaaaaagt gtagtattga gcggctcgct tcaataacta ttcagaggga
    15241 ttatggaaac acgtcgcgaa gagcgtatcg ggcagctgct gcaagaatta aaacgcagcg
    15301 ataagttaca tcttaaagac gccgccgccc tgcttggggt ttcggagatg acgattcgtc
    15361 gcgatctgaa caaccacagt gcgcccgtcg ttttgctcgg cggctatatt gttctggaac
    15421 cgcgcagtgc cagccattac ctgttaagcg atcaaaaatc ccgcctggtg gaagaaaaac
    15481 gccgggcggc aaaactggct gcgacgctgg tagaacccga tcagaccctc ttttttgact
    15541 gtggcaccac cacgccgtgg attattgaag cgattgataa tgaaatccct tttaccgccg
    15601 tttgttattc gctaaatacc tttctggcgc tgaaagagaa accccattgc cgcgcgtttc
    15661 tttgcggtgg tgaatttcac gccagcaacg ccattttcaa acccatcgat tttcagcaaa
    15721 cgctgaataa tttttgcccg gatatcgctt tctattctgc ggcgggcgtg catgtcagta
    15781 aaggcgctac ctgttttaat cttgaagagt tgccggtaaa acactgggcc atgtcgatgg
    15841 cgcaaaagca tgtgctggtt gtcgaccaca gtaaatttgg caaggtgcgt ccggcgcgca
    15901 tgggtgacct gaaacgcttt gatattgtgg tgagcgattg ttgcccggaa gatgagtatg
    15961 tgaagtacgc gcagacgcag cgcattaagt tgatgtatta atgacgtata accggatgac
    16021 gtttcgcgcc atccggttat cagaagatta agagaaccag ctgccgaacc actgatggaa
    16081 tttcatcatc acgaaatccc acacccgacc aaagaatccg ccctcttcca cattttccat
    16141 cacgatcagc ggacgctgct caatggattt accgttaagc tggaaatcaa tggtcccgac
    16201 aacctgacct tttttcagcg gtgcggtaag ctgcggttcc gttaacgtat aactcgcttt
    16261 caggtttttc agctgcccac gcggtatggt cactgagccc gcttcacctg ccccgagatt
    16321 cacttcgctc ttatcaccaa accagacgcg ctgagtcaca aaggtggcat caggtttaat
    16381 tggcgtcacg gtttcaaaga agcggaaacc ccaggtcaat aatttctcag actcattaaa
    16441 acggatacgg tcagttttcg cccccagcac tacggagatt aaacgcatat caccctgggt
    16501 agccgaagca accagattat atcccgcacc tgccgtggtt cctgtcttca tgccatcaac
    16561 attcagattg ctgctccaca gcagacggtt acggttaggc tgacgaattt tgttgaaggt
    16621 gaactctttc tctttatgaa tggcgtactc ttccggcaca tcgtggatca atgctttacc
    16681 cagcaatgcc atatcgcgcg cggtactgaa ctgccccggc gcatccagac cgtgcaccgt
    16741 ctggaaggta gtgttggtca gacccagttt tttggcataa ccattcatca aaccaataaa
    16801 tgactcctgg ctcccggcaa cgtaatcggc cagcgcaata caggcgtcat taccggactg
    16861 gataatcaca cctttgttca agtctgccac cgaaacctga tcgcccggtt tgaggaacat
    16921 taccgatgaa ccacgcagtg ccggatttcc cgtcgcccag gcatctttac cgaccgtcac
    16981 catatcggtg agtttaatct tatcggcctt aagcgcctgc ccaaccacat agctggtcat
    17041 gattttagtc aggctcgcgg gatccagttt ctcatccgcg ttgccttctg ccagcacttt
    17101 accgctggcg taatccatta aaatccatgc acgcgcatcc acgctcggcg cttcaacggt
    17161 ttgttccgcc gcgaatgccg ttggggcaaa aaggaataaa aatgcagaac ccgctgcaag
    17221 accacgaagg agagaggagt attgcgtcat aaatgccacc cgagtatcca ttcaaaaaaa
    17281 atacgttata tcgccgtatg ataagttcgg tgattaataa cgcacacaca aacttaaaga
    17341 aaccgtaagt tggtaaagtt tttaaagttt atgcaatatc ctgccgcttc gctgcttctg
    17401 gtgtcatttt tttgccagct gttgcttaaa agttaacttt acttcaccat cggagtcctg
    17461 ttcagcctgt ggctgcctgt cggaaaaggg gtgagtatga ttacgctgtg gggtcggaat
    17521 aattcaacta acgtaaaaaa agtattgctg acgctcgaag aactggaact accttatgag
    17581 caaattctcg cgggccgtga gtttgggata aatcacgatg ctgattttct ggcgatgaac
    17641 cctaacgggc tggtgccgtt gttgcgtgac gacgaaagtg atctcattct ttgggaatca
    17701 aacgccattg tccgctatct ggcggcacag tacgggcaaa aacgcctgtg gatcgactca
    17761 ccggcacgtc gtgcggaagc ggaaaaatgg atggactggg caaaccagac gctcagtaat
    17821 gctcatcgcg ggatcctgat gggattagtc agaacaccac cggaagagcg cgatcaggcc
    17881 gccattgatg ccagttgcaa agagtgcgac gccctgtttg ccctgctcga tgcggaactg
    17941 gcaaaagtaa aatggttctc tggcgacgag tttggtgtgg gcgatatcgc tatcgcaccg
    18001 ttcatctaca atttgtttaa cgttggcctg acctggacac cgcgtccaaa tctgcaacgc
    18061 tggtatcagc aactcactga acgccccgca gtgcgcaaag tggtgatgat tcccgttagc
    18121 taattgcgtg ggctaacttt aagtaattcc ccactggact cgtcggtgag aacgtataaa
    18181 taaccgtcgg gtccagtgcg aacatcacga attcgctgcc ctctgtccgt taaaatacgg
    18241 ccatcttctg tcactttgtc gccgttgacg ctcatcacaa tgacatcttt atctttcagc
    18301 gcgccaataa ataatttttg ctgccactgg gggaatttat cgctgttata gaaggccatg
    18361 ccgctcacag cgggcgaatc tttccagtaa aaaacaggtt gctcggtccc ggcgacgatc
    18421 tcccctttcg cttccggtat cttaaagcct gaatagttga ttccccaggt tgccagcggc
    18481 cagccgtagt ttttgccttt ttgcgggata ttaatttcat caccaccgcg cgggccatgt
    18541 tcattcagcc acagtgcatt actccacgga ttcatcgcca ttccttgcgg attacgaatg
    18601 ccataagacc agatctcggc gcgcgcaccg gattccttta taaaaggatt atcatccggg
    18661 atttagccct ggtcggtcag acgcaccagt ttgccctgta atttatccag atcctgcgcc
    18721 gtcgggcgct gattgttttc gcccagagca ataaaaagat aacctttacc gtcgaatacc
    18781 agccgcccgc caaaatggtt gccggtagac agttttggca tctggcgaaa gacggtgcgg
    18841 aagtcggtca cttttgagag atcatcactt aagcggccat aacccacagc agttccggct
    18901 ttgccatcat cgccaacttc ggaataactt aaccagatgc ggcgagactg agcaaaatca
    18961 ggcgctaaaa ccacgtccag caggccgccc tgcccgtgcg cccaaacgtc cggcactccg
    19021 gaaagcggcg cagataatcc ttttcctgct tgccagtgac gcaactcgcc gccgcgcaga
    19081 gtgattaaca taccgtgatt atcgggtaaa aaggccagtg accagggatg gtcgagtttg
    19141 tcttgcagta cttcgacatt taccgttgca ggagccgccc agagagcgga agaaagacaa
    19201 ataaggggca caaggaaaaa gaatggtcga tgcataacgc ctccggattg actctggctt
    19261 aaagcgtagt cagtggagga gataacaaat tcatttttac aaaaacttaa acatgaaggg
    19321 ggagacgctt tctccccctt agttttcagg ccttctcaag catggcgtgc ttctgcaggc
    19381 tctggatact cagcgttaag ctcatcagac aattttcaag cttatcggcg ttgacggtaa
    19441 taacagtcgg gcaatcatgg tgcccactca tcaaacatac tgcggctgtc gctaatgctt
    19501 cttcagcatg atgaagagca ctccactctt cctgatccag atgaagattc aaccgcagcg
    19561 atttatcgtg cagttcgcga ttcagtttaa aaaagttatc tcgtagatga ttgctttcgc
    19621 tgacggacat gtatcctttt gccttcctca gttgcacata tgcggcgagg tcaacgcgtg
    19681 cgtcgatcaa ccggttcagc agatcgattc gctgtctgtc aacgaacata cttcgatcct
    19741 cctctttcca gccttgttgc aattaaatag tatggctatt ttttgagcaa aatgctgtcc
    19801 aggatcaatt cttcacgccg aattaacaaa atttacattt ccttcgcctc cgccagcgaa
    19861 tgagattgcg atacaaattt tatccagaac tctgtagaat ccctgccctg acctttcctg
    19921 taactgaata ttttttaagc tatgcgcaaa gtaactcccc agccgaaaat cggctttgtt
    19981 tcccttggct gtccgaaaaa ccttgttgat tcagagcgta ttctcaccga actccgcact
    20041 gaaggttatg acgtggtacc gagctatgac gatgcggaca tggtgatcgt caacacctgc
    20101 ggctttattg acagcgcggt acaagaatca ctggaagcca ttggtgaagc gttgaatgaa
    20161 aacggcaagg ttattgtgac cggttgtctg ggggcaaaag aagatcagat ccgcgaagtc
    20221 cacccgaaag tgctggaaat caccgggcct catagctatg agcaggttct ggagcacgtt
    20281 catcactacg tgccaaaacc gaaacacaac ccattcctga gcctggtgcc agaacaaggt
    20341 gtgaaactga cgccgcgtca ttatgcctat ctgaaaattt ctgaaggctg taatcaccgc
    20401 tgcaccttct gcattattcc gtctatgcgc ggcgacctgg tgagccgtcc gattggcgaa
    20461 gtgttaagtg aagcgaaacg tctggtagat gcgggcgtta aagagattct ggtgatctcg
    20521 caggatactt ccgcctatgg cgtcgatgtt aaacatcgta ctggcttcca caacggcgag
    20581 ccggtaaaaa ccagcatggt cagcctgtgc gaacagttat cgaaactggg gatctggaca
    20641 cgtctgcact acgtttaccc ttatccgcat gtggacgacg tcatcccact gatggcagaa
    20701 ggcaaaatcc tgccgtatct ggacattccg ttgcagcacg ccagcccgcg cattctcaaa
    20761 ctgatgaagc gtccgggttc tgtagatcgc caactggcgc gcatcaaaca gtggcgcgaa
    20821 atctgcccgg aactgaccct acgctcaacc tttattgtcg gcttccctgg cgagacggaa
    20881 gaagatttcc agatgctact cgacttcctg aaagaagcgc gtctggatcg cgttggctgc
    20941 tttaaataca gcccggttga aggtgcagac gccaatgccc tgcctgacca ggttccggaa
    21001 gaagtgaaag aagaacgctg gaaccgtttc atgcagctgc agcagcagat ttccgccgag
    21061 cgcctgcaag agaaagtggg ccgtgaaatt ctggtgatta tcgacgaagt ggacgaagaa
    21121 ggcgcgattg gtcgcagcat ggcagatgca ccggaaatcg acggcgcggt ttatctcaat
    21181 ggtgaaacca acgttaagcc gggtgatatc ctgcgtgtga aagtcgagca cgccgatgag
    21241 tacgatttgt ggggtagccg ggtttaatta attcaggccg ggcaaaatca tttgtccggc
    21301 ctgcgaataa tgattactgc cagctaatct gtgccctgcc gtttttctta ctcttataca
    21361 gccgctcatc cgccagcatt ttcaagcgtt cgacggtggt acattcattt gaatgcgcta
    21421 caccgatact ggtgcttaaa ttaatgcttc ccacctggaa atgaacgatt tcttttagaa
    21481 tcgatagcaa ctcttccagc gtgccgccag tataaatcac cgcaaattcg tcaccgccga
    21541 agcggtagac tttaccttta tctaccggga tctgtgattt aaggattttc accactttca
    21601 tcaacacttc atcgcccagc aaatgaccga aggtgtcgtt aatgtttttg aatttatcga
    21661 cgtcaaacag gcacaaataa ccgccttttt ccggcagcgc ttttaaatct tcgtcaaagg
    21721 attttctgcc cattgctttg gtcaggtcgt ccaggaacgc cgagttatac atactgtaga
    21781 gttttttgac atcggcagcc gaaaagaaaa gcccgccatc cagtacgcgt tcaaaatggt
    21841 tattaataat ggcttctttc tccacaatac cacgattaat cactctgcgt accagataga
    21901 ggcaactggc acccgatatc actgccacca atgcggcaaa gaaaataatc aatgtacaga
    21961 cccgcaaata aacatctttt ttatacgcaa atacgtacaa tccggtcggc aaataggcga
    22021 agtgctccat cgcttccgaa acggtgaaga tggcgttttt agcattatat tcatcaccgg
    22081 tcaccggttt accgttctta aacagagaga acatcaggtt ctcgctgtcc atcgccagcg
    22141 attttgacag taaagaaaaa cgctccagac caagcaaaaa tatcagacta cgattgtgag
    22201 ttaccggata gaggtaatag acgaaggttt taccgtcaaa agagataaac cctgtatttt
    22261 tgacatgtcg atacttcaca acctgcataa tatctgcaca ataatttttc tcgaactcct
    22321 ttacctgaat acattcgtca ctggaaatca gataaggacc gacatcgtac aaataagagg
    22381 gtgaaaccga gtcggcgtaa gctgtgcgca aaccaaccaa cagctgttcc tccgtgggat
    22441 ttagcgtgtt gataagatca attgccacgc tacgccgata ttcagcaata tttgccaccg
    22501 caaaagcagt atcaataacg gactgtttca ggctatacac ccgttccttt accatttgag
    22561 tgtagatccc gcaggcaact gctgaaatca tgataaaaat cagcagactg actgtaagta
    22621 cgaacttatt gattctggac atacgtaaac tactctttta ctaattttcc acttttatcc
    22681 caggcggaga atggcatggg tttctggtac agataccctt gggcctgaac acatcctaat
    22741 ttgattaatt cgcgcgcaac gtcttcgctc tctacgcctt ccgcaatcac cgggatccgg
    22801 ttatagcgtg ccagatcgaa aataatgcgc acaatttttc ccgcagtgcc gaaaatatcg
    22861 ccggtaaatt gcgagtccac ttttatgcta tctggattaa tttcataaaa acgtttcagg
    22921 tttgacaaac cggtgccaaa gtcatcaatc gatatccgta ccccttgttt acgcagttgt
    22981 tcaatattga gataaaaatg ttcattaatt cgctcgatca cggtttcagt gatctcaaca
    23041 cagaggctgt tcgccttcag gccattttct ttcatgatgg tggtaaaacg ctcatgaaag
    23101 tgcggttcgt ttaactgcca gggcgaaaca ttaatatgca gtaagaaatc atccttcagc
    23161 gacgcacgct gactaaaatg tctgaattca cccattgccg ttttaataat ctgataaccc
    23221 agctcattga taaacccgat atcttcagca atggtaatga acttcagcgg tgaaatgatc
    23281 ccccgctccg cagaaaccca acggcacaac gcctccgcgc ccagaatggc gcgggtgtta
    23341 atatcaacga tcggttgata gacgaggaat agttcaccaa tttccattgc atggcgaata
    23401 ttacgcgcaa tccagatatt ctcttctatt tcgttgagtt tcgcgtcatc acagaaaata
    23461 aactcggaga acatatggtc acggatactg gaaatcgcca gtttcagttt ttctgcgtaa
    23521 ctccaggcgt tttccccctg caaaggttca caaatggcag acttatggat gcgcagattg
    23581 cggtagccaa agctgctgtc aaaatcgttt accatcgcta ttttacgggt aaatgtctgt
    23641 acattttccc gacacagaag taaatagaga tcggcattga atgtcacgat atcgcagcat
    23701 tgcgcatatt tctcggcaat tcgctgacac aatttcctga cgattttatt gcgttcatca
    23761 acaccaaaca ccgcttccac ggcttccaga ccacacagtt tgatgatccc cacagccaga
    23821 tactgattat tgttggactc aataatcgcc atatttttaa taaaaccgct ttcctgatcg
    23881 taagagatca gtgagagcaa tttatttgac cgttcaattt cggcatcttt catttccgcg
    23941 atcgaatttt tgatgctgat aatctctttc gcatcgcctt cgcgaatttt cagagcctcg
    24001 atctgatccg ttgagtcggg caaaaacgcc agcgtattaa ccagctcggt catttcagaa
    24061 aattgccgac ggaacacacg agtggtcatg aaatatacca ccagcaacac gataataatg
    24121 aaagcaaacg gtagataaaa aagtttatct gatgcgttca ataaattacc gccaggataa
    24181 taaaaataga tttcgaacaa tgcattattt ggcactgctg atgtctttgc aatatactcc
    24241 gtatcgacag aatcaataat attataatta ccttcgctat tataaataat cagtttttca
    24301 cgagcgagtt gattcttggc aatgatctca tgaccttgtt tcaaaaatag aacttcatca
    24361 ttagcgctga tttgatcaag atactcgttc agataccgcc agtttaaatc gacatgaatt
    24421 ttatctaccg ctaacaaatc gtgatctttg ccgttgatac gtagcgtaaa agagatacat
    24481 tttttacccg gacgatcaaa gcaattgtat gccggattcc agctgttgtt agagtcatct
    24541 gaagccaccg accacggtcg tttcgatggg ttaaaatcgg caaaaataac ccgccctgac
    24601 tcgtcgacaa gcggtccatt tacttgttcc tgatgataaa caacgatttt atcattcgcc
    24661 ctccgcgaaa agagataata tttattatca tcaagtacca gtcccaccga gtctaaaaat
    24721 ggcatcattt ccattttcag aat
//
LOCUS       DAJOSY010000066        24645 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJOSY010000066 DAJOSY010000000
VERSION     DAJOSY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.066"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(139..1020)
                     /gene="prmA"
                     /locus_tag="OHN27_002767"
     CDS             complement(139..1020)
                     /gene="prmA"
                     /locus_tag="OHN27_002767"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCQ2287175.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1032..2483)
                     /gene="panF"
                     /locus_tag="OHN27_002768"
     CDS             complement(1032..2483)
                     /gene="panF"
                     /locus_tag="OHN27_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCQ2287176.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2473..2715)
                     /locus_tag="OHN27_002769"
     CDS             complement(2473..2715)
                     /locus_tag="OHN27_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCQ2287177.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLSGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2824..4173)
                     /gene="accC"
                     /locus_tag="OHN27_002770"
     CDS             complement(2824..4173)
                     /gene="accC"
                     /locus_tag="OHN27_002770"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCQ2287178.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPNPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4184..4654)
                     /gene="accB"
                     /locus_tag="OHN27_002771"
                     /gene_synonym="fabE"
     CDS             complement(4184..4654)
                     /gene="accB"
                     /locus_tag="OHN27_002771"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCQ2287179.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5632..6606)
                     /locus_tag="OHN27_002772"
     CDS             complement(5632..6606)
                     /locus_tag="OHN27_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCQ2287180.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRVLPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6758..8698
                     /gene="csrD"
                     /locus_tag="OHN27_002773"
     CDS             6758..8698
                     /gene="csrD"
                     /locus_tag="OHN27_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCQ2287181.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSVLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9003..10046
                     /gene="mreB"
                     /locus_tag="OHN27_002774"
     CDS             9003..10046
                     /gene="mreB"
                     /locus_tag="OHN27_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCQ2287182.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10112..11215
                     /gene="mreC"
                     /locus_tag="OHN27_002775"
     CDS             10112..11215
                     /gene="mreC"
                     /locus_tag="OHN27_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCQ2287183.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11215..11703
                     /gene="mreD"
                     /locus_tag="OHN27_002776"
     CDS             11215..11703
                     /gene="mreD"
                     /locus_tag="OHN27_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCQ2287184.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11712..12305
                     /gene="yhdE"
                     /locus_tag="OHN27_002777"
     CDS             11712..12305
                     /gene="yhdE"
                     /locus_tag="OHN27_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCQ2287185.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12295..13764
                     /gene="rng"
                     /locus_tag="OHN27_002778"
     CDS             12295..13764
                     /gene="rng"
                     /locus_tag="OHN27_002778"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCQ2287186.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13832..17632
                     /gene="yhdP"
                     /locus_tag="OHN27_002779"
     CDS             13832..17632
                     /gene="yhdP"
                     /locus_tag="OHN27_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCQ2287187.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVNNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGSDIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINSDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNIELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            18062..19507
                     /gene="tldD"
                     /locus_tag="OHN27_002780"
     CDS             18062..19507
                     /gene="tldD"
                     /locus_tag="OHN27_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCQ2287188.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSTQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19641..20570)
                     /gene="aaeR"
                     /locus_tag="OHN27_002781"
     CDS             complement(19641..20570)
                     /gene="aaeR"
                     /locus_tag="OHN27_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCQ2287189.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20753..20956
                     /locus_tag="OHN27_002782"
     CDS             20753..20956
                     /locus_tag="OHN27_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCQ2287190.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20964..21896
                     /gene="aaeA"
                     /locus_tag="OHN27_002783"
     CDS             20964..21896
                     /gene="aaeA"
                     /locus_tag="OHN27_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCQ2287191.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21902..23869
                     /gene="aaeB"
                     /locus_tag="OHN27_002784"
     CDS             21902..23869
                     /gene="aaeB"
                     /locus_tag="OHN27_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCQ2287192.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23961..24233
                     /locus_tag="OHN27_002785"
     CDS             23961..24233
                     /locus_tag="OHN27_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCQ2287193.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24289..24552)
                     /gene="yhcN"
                     /locus_tag="OHN27_002786"
     CDS             complement(24289..24552)
                     /gene="yhcN"
                     /locus_tag="OHN27_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCQ2287194.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5838 a         6235 c         6681 g         5891 t
ORIGIN      
        1 aaatttgagt aagttctcaa tttttcttta tgaatgaaaa ttttggcacg caaatttgcg
       61 taaataatcg gcaaatttac gtgccttttg tgagcttgct cgcacttcgc cccgcgtcac
      121 cctacggcga tgcgaaggtt aattcttacg accggtaata cggcaccact cttctttttc
      181 cacgaccggg tccagtgcga agctatcggc ataagcttca caaacgctct ctgcctggct
      241 tgccagaata ccggaaaggc ccagcaaacc gcctgaaacc ggcaggacgc tgattaacgg
      301 tgccagttca cgtaatgggc ctgcaaggat gttagcgacc accacgtcgg ctttcatttc
      361 ttctggctga tctttcggta agtagagttc cagacggtca gaaacgccat tacgttcggc
      421 gttatcgcgg ctggcctgaa tcgcctgcgg atcgatatca ataccaatgg cttttgctgc
      481 acccagtttc agcgccgcga tcgccagaat gccggaacca cagccaaagt cgatgactgt
      541 tttaccggtt aaatcgaggc tgtcgagcca ttgcaggcac agagaggtgg ttggatgggt
      601 acccgtacca aacgccagcc ctggatctaa catcacgttg acggcgtttt cgtccggcac
      661 atcacgccag ctagggcaga tccacagtcg ttcaccaaag cgcatcgggt ggaaattatc
      721 catccattcg cgctcccagt ctttatcttc tagttgttcg attttatgcg cgaagcctgc
      781 gccgagcagc ggatggtttt ccagaatcgc caccacgtcg ttcatatcgg tttcagcgtc
      841 gaacagacca atcacatcgg tgtcgcccca caggcgcgtt tcgcccggca gcggctcaaa
      901 tactggcgta tcgtgggtat cctgaaaagt gatagaaacg gcacccgctt ccatcagcgc
      961 atcgctaaga tcttccgcgt tcgcgccggt ggtgttcagt ttcagttgga tccaaggcat
     1021 ggcaaaactc tttatttatc agtagtcaaa acggtagctt gcgggacgga tgtaccgaaa
     1081 cggtttccga ccaggaaagc cagcaaactt agtagtaacg agggcacgat agggtggaag
     1141 cccaggtact gaatattcag cgtcgcgagt acggcataca gcacgccgcc aacgatcatc
     1201 gcacttagcg cgcctttggc gttggcgcgt tcccagtaaa gacccagcac cagcggccac
     1261 aggaaaacgg cttccagccc accgaaggcc agcaaattca gccagatgat catttctggc
     1321 ggcttccagg cggcaagcag cagcaacgcg ccgagaacta acgtaattac cgccgacatc
     1381 cgcttcagac gcgtctcgtt ttgcatttga tccggacgga tattcagata gagatcttta
     1441 atgatcgtag cggaactttg cagcagttgg gcgttaattg tcgacatgat cgcagccatc
     1501 ggtgcagcca ggaagatccc ggcggcaaac ggtggcagca cttttaccat taacgtcggg
     1561 atcaccagat ccggtacggt gagatcgggg atcaccgccc gacctaacgc tccggccagg
     1621 tgcataccga acatcaggat tgcgactaca atcgtaccga tgatgatccc ccggtgtact
     1681 gctttgctgt ctttataaga gatacagcgc accgcagtat gcggcaggcc tataacgcca
     1741 aagcacacca gtacccagaa cgacgtcata aaggcaggcg acagaatatc gtcagcgcct
     1801 tgtggcgtaa ccagttgcgg atcgatggtt tgcaaggtct gtactgcgtt gcttaagccg
     1861 ccagcggcat gtaccacgcc aataagcagc acaacggtgc caatcagcat cacaagccct
     1921 tgcatggtgt cgttcagcac gctggcgcga aagccaccaa aggcggtata taacgcaatg
     1981 ctgataccaa aaatcagcag cccggtttca taaggaatac ccgccgcggt ttccagcagg
     2041 cgcgcaccgc cgataaactg cacggtcatc gcaccaacga acgcaaccag caaactcaaa
     2101 ctcgccagcc acaccagaag acgactctgg tagcgggcaa acagcatatc gtttagcgtc
     2161 actgcattgt agcggcgcgc aagaatcgca aacttcttgc cgagaatacc gagtgaaagc
     2221 cagactgcag gaagctgaat catcgccagc aatacccagc ccagcccgta tttataagca
     2281 gctcctggcc cgccgataaa cgaactggca ctgatatagg tcgcggtgag cgtcatcgcc
     2341 agcacaatac cgcccataga gcggctgccg aggaaatact cattaaggaa ggtgccggtg
     2401 ctccgtttac gcatcgcata aaccgagata ccgaacacca ccaccagata ggcgaccagc
     2461 ggtagaatta cttcaagctg catcgtcatc ctccagtggg atatcgcgat agataaattt
     2521 caccatcgcc cagcacagtc caataaacag cagcggcgtc aggatgcagg ccatctcaaa
     2581 ccagcgcgga aagccggtaa aaccgggggc aacgccagat aagtaagcgg ctactaacca
     2641 aactgccaga tacaaaaggg tcagccccag cgcccagcgc gcctctttat gggcctgaac
     2701 aaaacgagtg tccatttttt gtccctgatg ggtgaagaaa gcggggattg taccttatgg
     2761 gggttgtcga tccccagtaa taaaaaaggc cggaaaatcc ggccttttga cgctttagca
     2821 gtcttatttt tcctgaagac cgagtttttt ctccagatag tggatgttag tgccaccatg
     2881 ctggaagttc tcgtcattca tgatgcggat ctgcagatca acgttggttt tgataccgtc
     2941 gatgatcagc tcctgcagcg cattcttcat gcgggcaatc gccacgtcac ggttttcacc
     3001 gtagcaaatc agcttaccga tcattgagtc atagtacggc ggtacggtgt agcccgcgta
     3061 gatatgagac tcccaacgta cgccaaaacc gccaggtgcg tggaaacggg tgattttgcc
     3121 tgggtttggc aggaaggtgt tcggatcttc ggcgttgata cgacattcca ccgcatggcc
     3181 gcgaacgtga acttcttctt gcttgatcga cagcggttga ccggcagcga tacgcagctg
     3241 ttctttgatc aggtcaacgc cggtgatcat ttcggtaacc gggtgttcta cctgaatacg
     3301 ggtgttcatt tcgatgaaat agaactcgcc gttttcgaac aggaactcga aagtacctgc
     3361 accgcgatag ccgatatcaa cacacgcttt agcgcaacgt tcgccgatgt agcgacgcag
     3421 ttccggggta atgcccggtg ctggcgcttc ttcgaccact ttctggtggc ggcgctgcat
     3481 ggagcagtca cgttccgcca gatagatagc gttgccctga ccgtcagcca gtacctgaat
     3541 ctcgacgtgg cgaggatttt ccaggtattt ctccatgtaa accatatcgt tgctgaaagc
     3601 agctttcgct tccgcacggg tcatggagat ggattgtgcc agttcagcgt cgccgcgcac
     3661 tacgcgcata ccgcgaccgc cgccgccgcc ggaggctttg ataatcaccg gataaccaat
     3721 gcgtttagca atggcgcggt ttttatccat atcgtcgccc agcgggccgt cagaaccagg
     3781 tacgcaagga acgcccgctt ttttcatcgc cgcgattgcg gatactttgt cgcccatcag
     3841 gcgaatggtt tctgctttcg ggccgatgaa gataaagccg gagcgttcaa cctgctcggc
     3901 aaagttggcg ttctcggaga ggaagccgta acccggatgg attgctactg cgccggtgat
     3961 ttcagcggcg ctgatgattg ccgggatgtt cagataactt tttactgacg gagcagggcc
     4021 aatacagacc gtttcatctg ccagtaatac gtgttttaga tcgcgatccg cgctggagtg
     4081 cacagcgaca gtcttgatgc ccagttcttt acaggcacga agaatacgca atgcaatctc
     4141 gccgcggttg gcaataacaa ttttatccag catgttcgcc tcgttactcg atgacgacca
     4201 gcggctcgtc aaattctacc ggttgtccac tttcgaccag aattgctttc acggtaccgg
     4261 atttgtccgc ttcgatctgg ttcatcattt tcatggcttc aacgatgcac agggtatcgc
     4321 ccacgttgac tttctgaccc acttcgatga acgcttttgc gtccgggctt ggggtgcggt
     4381 agaaagtacc aaccatcggg gaacgtacga tgtgaccact gatttccgct gctgctggcg
     4441 cttccatgga aggaacggtc gccggagcgg ctgcgttaga ttgagctggc tgctgcatca
     4501 ttggtgcagc gtaagcttgt tgcatcacag ggaaacttgc ggcaggagct gcacggctaa
     4561 tgcgtactga ctcttcgcct tcagaaattt ccagttcgga gatgcctgat tcttcaacca
     4621 gctcgatcag ttttttaatc ttacgaatat ccatgagtgg gttccgtact ctttgtttag
     4681 tgtgattgtg acaggcgttt caccgccgtc tgtaaagcgt atgccccgac agctgtatgc
     4741 atagcgataa attccagcag gccgggacgc cggtctattt tgccccagag tgtcaatgtt
     4801 agacttgacg gacattgtgc agcgatctgc tgctatcctc cggcaaaaaa caaaatatac
     4861 cttcgttgcg ccaatgtcac cttttccgca gcgcaaaaac tgcgtcaggg aggacgaggt
     4921 cgcacattat aacgatttcg tagcaattgg cagctaaata ctggtcttat cagggaagat
     4981 aatcaacagc taacatgtaa ataaccttca acaccgtgta atttgcaaca agccgcacaa
     5041 ttcacgaaat tagcgccatc atcgacggaa cttcttataa cgtaaggtga aaagctgaaa
     5101 agccagccct gcgaagatga ggggctgcaa tgagataatc ttcacagacc acaaataatg
     5161 tagggatgcc aggatcgcga caagatagac gacattacgc agcggttgct ggggattacc
     5221 caattttcac tgcatcgtct ggatagatgt gaatgctaaa gcacgcaaaa ttaaccgatt
     5281 gacaatgctc agcattaaat aggttcgagt gattaatttg tagtcaaaca aaatcagatt
     5341 attcacccca tggtaccaga aataagtaag gggctaagcg tagcgtcgcc caggcaaagc
     5401 accataaacc taacaggcga cggaggcgta tcaataatag ctgtttagtg gagtatcaat
     5461 gcgcatcaat ttgtggtcag tcgaaatcat gactttccag tttggatgat gttgtataac
     5521 ttcctgacca aggtgttcga cgaaagctgt ccggctaacc gcttttcccg ttaaccgatt
     5581 gaataatgat ttcaaaatga gttccctctc ttattattcc ctgctaaatg gttagttaac
     5641 cttcaccagc gtgcgaccct ggatctggtt attaatgatg gcctcggcaa agttcggtgc
     5701 ctctgacaga gatatctctt ttgccgcctg ggtatagaat gattccggta aatcggcgac
     5761 cagtcgctgc caggcttgtg cgcggcgttc tggtggcgtc attactgaat ccaccccttg
     5821 caaacggaca ttacgcagaa taaatggcat gaccgtggtt ggcagagtaa aaccacccgc
     5881 cagaccacag gccgccacgc agccgccgta attcatttgc gccagcactt ttgccagcac
     5941 tttgtcgcca acggtgtcaa ttgccccagc ccagacttgt ttttccagag gacgggattc
     6001 ggcaaactca tcacgaggga gaacacggct agcacctaaa cttttcagat attcatgggt
     6061 actttcgcga ccggaaacgg caacgacctg ataacccaac ttatgcagca gcgccacggc
     6121 ggtactgccg acgccaccac tggcacccgt cacgacaatc tccccgtcct gcgggcgaac
     6181 accggcatct tccagcgcca tcacacacag catggcggta aaaccggcag taccgataat
     6241 cattgcttta cgcgcgtcca gcccttgcgg catggcaacc agccagtcac ctttcactcg
     6301 cgcctgctcc gccagcccac cccagtggtt ttcaccaacg ccccagccag tgagtaacac
     6361 ctcctgaccg gcatgaaaac gcggatcttc gctggtgcgt acagttccgg caaaatcgat
     6421 cccaggaatc atcggaaaat tacggatgat ttttccctta ccggtaatcg ccagcgcatc
     6481 tttatagttc aggctcgacc agtgaacatc gaccgtgaca tcgccctccg gcaggcgact
     6541 ttcgtccaga gtctgtactg atgcgagagt tttgccgtcc tgctgttcta aaagtaacgc
     6601 ctgcataagt ggtcctcatg tgcatgatgg attggaaaat aatttctgaa gactatactc
     6661 gctaatggaa aagcaaagcc gatgaagcgc aataaattgc gagataaatc tgatttgcta
     6721 gtatgcccgc ttcctcacta tcggagttaa cacaaggatg agattaacga cgaaattttc
     6781 ggcctttgtt acgctgctca ccgggttaac aatttttgtg actttgctgg gctgttcgct
     6841 aagtttctac aacgccattc agtataagtt tagtcatcgc gttcaggcgg tggcgacggc
     6901 gattgatacc caccttgtgt cgaatgactt cagcgtatta aggccacaaa ttaccgaatt
     6961 aatgatgtcg gcagatatcg ttcgtgtaga cctgctccat ggtgataaac aggtttatac
     7021 cctggccaga aatggtagtt atcgtccagt tggctccagc gatctgtttc gcgaactgag
     7081 cgttccgttg ataaagcatc cggggatgtc gttgcgtctg gtttatcagg atccgatggg
     7141 caactatttc cattcgttga tgaccaccgc gccgctcacg ggggcgattg gctttatcat
     7201 tgttatgctc ttcctggcgg tacgctggtt acaacggcaa cttgccgggc aagaattgct
     7261 ggaaacccgg gctactcgta tcttaaacgg tgagcgtggc tctaatgtgt tgggaaccat
     7321 ctatgaatgg ccgcccagaa ccagcagtgc gctggatacg ctgcttcgtg aaattcagaa
     7381 cgcacgcgaa caacacagcc gtcttgatac gctgatccgc tcttatgccg cccaggacgt
     7441 gaaaaccggc ctcaataacc gactcttttt cgataatcag ttagcaacgt tactggaaga
     7501 tcaggagaaa gtaggtaccc acgggatcgt gatgatgatt cgtctgccgg atttcaatat
     7561 gttgagcgat acctgggggc acagccaggt tgaagaacag ttcttcactc tgacgaatct
     7621 gctgtcgaca tttatgatgc gctaccctgg cgcactgctg gcgcgttacc accgcagtga
     7681 ttttgctgcg ctgttaccgc accggacgtt aaaagaggca gagagcatcg ccggtcagtt
     7741 aatcaaagcc gttgatacct tgccgaacaa taaaatgctc gatcgcgacg atatgatcca
     7801 cattggtatc tgcgcctggc gtagtggtca ggataccgag caggtaatgg aacatgcaga
     7861 gtctgccacg cgtaatgcgg gattgcaggg cggcaatagc tgggctattt acgatgactc
     7921 gttgcctgaa aaaggacgcg gtaatgttcg ctggcgtacg cttatcgagc aaatgctcag
     7981 tcgcggcggc ccgcgccttt atcaaaaacc ggcggttact cgcgaaggtc aggttcatca
     8041 tcgcgaactc atgtgccgca tcttcgatgg taatgaagag gttagctcgg cggagtatat
     8101 gccgatggtc ttgcagtttg gcttatcgga agagtatgac cgtctgcaaa tcagccgtct
     8161 tattccacta ttgcgttact ggccagagga aaatctggcg attcaggtta ccgttgagtc
     8221 gctgattcgc ccgcgttttc agcgttggct gcgcgatacg ttaatgcaat gtgaaaaatc
     8281 acaacgaaaa cgcataatta ttgaacttgc agaggccgat gtaggtcaac atatcagtcg
     8341 tttacaacct gttattcgtt tagtgaatgc tttaggggta cgggtagccg tcaaccaggc
     8401 tggtttgacg ctggtaagta ccagttggat caaagaactt aatgttgagt tactcaagct
     8461 ccatccgggg ctggtcagaa acattgagaa gcgaacggag aaccagctgc tggttcaaag
     8521 cctggtggaa gcctgctccg ggaccagcac ccaggtttac gccaccggcg tgcgttcgcg
     8581 aagcgagtgg cagaccctga ttcagcgcgg tgttacaggc gggcaagggg attttttcgc
     8641 gtcctcacag ccacttgata ctaacgtgaa aaaatattca caaagatact cggtttaacc
     8701 tgccgtttaa tccgttttca cgtagaataa cgcgcgctgc gtctcatggg agtgtgcttg
     8761 tctgctcgcc agattgttgc agcacatatg cagatgaatg accttacgcg gttgcaaaca
     8821 ggcgaggaat gctgctgatg cattaagcct ttctggactc aggcagagat ttgtaacaaa
     8881 ggaaacgaac tgcactaatt ttcaccgtag cagatgattt ttgcgccttg tcgctgctgc
     8941 gtgtggttgg taaagtaagc ggattttctt ttccgcccca gctttcagga ttatccctta
     9001 gtatgttgaa aaaatttcgt ggcatgtttt ccaatgactt gtccattgac ctgggtactg
     9061 cgaataccct catttatgta aaaggacaag gcatcgtatt gaatgagcct tccgtggtgg
     9121 ccattcgtca ggatcgtgcc ggttcaccga aaagcgtagc tgcagtaggt catgacgcga
     9181 agcagatgct gggccgtacg ccgggcaata ttgctgccat tcgcccaatg aaagacggcg
     9241 ttatcgccga cttcttcgtg actgaaaaaa tgctccagca cttcatcaaa caagtgcaca
     9301 gcaacagctt tatgcgtcca agcccgcgcg ttctggtttg tgtgccggtt ggcgcgaccc
     9361 aggttgaacg ccgcgcaatt cgtgaatccg cgcagggcgc tggtgcccgt gaagtcttcc
     9421 tgattgaaga accgatggct gccgcaattg gtgctggcct gccggtttct gaagcgaccg
     9481 gttctatggt ggttgatatc ggtggtggta ccactgaagt tgctgttatc tccttgaacg
     9541 gcgtggttta ctcctcttcc gtgcgcattg gtggtgaccg tttcgacgaa gctatcatca
     9601 actatgtgcg tcgtaattac ggttctctga tcggtgaagc caccgcagaa cgtatcaagc
     9661 acgaaatcgg ttcggcttac ccgggcgatg aagtccgtga aatcgaagtt cgtggccgta
     9721 acctggcaga aggtgttcca cgcggtttta cccttaactc caacgaaatc ctcgaagcac
     9781 tgcaggaacc gctgaccggt attgtgagcg cggtaatggt tgcactggaa cagtgcccgc
     9841 cggaactggc ttccgacatc tccgagcgcg gcatggtgct caccggtggt ggcgcactgc
     9901 tgcgtaacct tgaccgtttg ttaatggaag aaaccggcat tccagtcgtt gttgctgaag
     9961 acccgctgac ctgtgtggcg cgcggtggcg gcaaagcgct ggaaatgatc gacatgcacg
    10021 gcggcgacct gttcagcgaa gagtaatcgg atgcaggcag gggaagcgtc tgtttaccct
    10081 gcctggtctg atacgagaat acgcataact tatgaagcca atttttagcc gtggcccgtc
    10141 gctacagatt cgccttattc tggcggtgct ggtggcgctc ggcattatta ttgccgacag
    10201 ccgcctgggg acgttcagtc aaatccgtac ttatatggat accgccgtca gtcctttcta
    10261 ctttgtttcc aatgctcctc gtgaattgct ggatggcgta tcgcagacgc tggcctcgcg
    10321 tgaccaacta gaacttgaaa accgggcgtt acgtcaggaa ctgttgctga aaaacagtga
    10381 actgctgatg cttggacaat acaaacagga gaacgcgcgt ctgcgcgagc tgctgggttc
    10441 cccgctgcgt caggatgagc agaaaatggt gactcaggtt atctccacgg ttaacgatcc
    10501 ttatagcgat caagttgtta tcgataaagg tagcgttaat ggcgtttatg aaggccagcc
    10561 ggtcatcagc gacaaaggtg ttgttggtca ggtggtggcc gtcgctaaac tgaccagtcg
    10621 cgtgctgctg atttgtgatg cgacccacgc gctgccaatc caggtgctgc gcaacgatat
    10681 ccgcgtaatt gcagccggta acggttgtac ggatgatttg cagcttgagc atctgccggc
    10741 aaatacggat attcgtgttg gtgatgtgtt ggtgacctcc ggtctgggcg gtcgtttccc
    10801 ggaaggctat ccggtcgcgg ttgtctcttc cgtaaaactc gatacccagc gcgcttatac
    10861 tgtgatccag gcgcgtccga ctgcagggct gcaacgtttg cgttatctgt tgctgctgtg
    10921 gggggcagat cgtaacggcg ctaacccgat gacgccggaa gaggtgcatc gtgttgctaa
    10981 tgaacgtctg atgcagatga tgccgcaggt attgccttcg ccagacgcga tggggccaaa
    11041 gttacctgaa ccggcaacgg ggatcgctca gccgactccg cagcaaccga cgacaggaaa
    11101 tgcagctact gcgcctgctg cgccgacaca gcccgctgct aatcgctctc cacaaagggc
    11161 tacgccgccg caaagtggtg ctcaaccgcc tgcgcgtgcg ccgggagggc aatagtggcg
    11221 agctatcgta gccagggacg ctgggtaatc tggctctctt tcctcattgc gctgttgctg
    11281 caaatcatgc cctggccgga taacctgatt gttttccggc caaactgggt gttacttatc
    11341 ttgttgtatt ggatcctggc cttgccccat cgcgtaaatg tgggcacagg ttttgtgatg
    11401 ggtgccatac tggatctgat cagcggctcg acgcttggcg tacgcgtatt ggcgatgagc
    11461 atcattgctt acctggtggc gctgaaatac cagcttttcc gcaacctcgc attatggcag
    11521 caggcgctag tcgtcatgtt gctttcgctg gtggtggata ttattgtttt ctgggcagag
    11581 tttttagtga ttaacgtctc tttcagaccg gaagtgttct ggagtagtgt agtcaatggg
    11641 gtgctctggc cgtggatttt cttgctgatg cgtaaagtcc gtcagcagtt tgcagtgcaa
    11701 taaaggtttc tatgacttct ctgtatttag cttccggttc tccgcgtcgt caggagttac
    11761 ttgcgcaact tggcgtgacc tttgaacgta ttgttacggg cattgaggag cagcgtcagc
    11821 cgcaggagag cgcgcagcag tatgttgtgc gtctggcacg cgagaaagca caagcgggtg
    11881 tcgcgcaaac ggcgcaggat ctcccggtgc tgggtgcgga tactatcgtt atcctgaacg
    11941 gagaagtgct ggagaaaccg cgcgacgcag agcatgcggc gcagatgttg cgcaaattat
    12001 cgggtcagac ccatcaggtg atgacggcag tggcgttggc tgacagccag cacattctcg
    12061 attgcctggt ggtcaccgat gtgactttca gaacgttaac agacgaagac atcgcgggct
    12121 atgtcgccag cggtgaaccg ttagataaag caggtgcata cggtattcag gggctgggtg
    12181 gctgttttgt caggaagata aatggcagct atcacgccgt agtcggctta ccgctggttg
    12241 aaacgtatga attattaagt aattttaacg cactgcgtga gaaaagggat aaacatgacg
    12301 gctgaattgt tagtaaacgt aacgccttcg gaaacgcgag tggcgtatat tgatggcggt
    12361 attctgcagg aaattcatat tgaacgtgag gcgcgacgcg gaatagtagg caatatctac
    12421 aagggtcgtg taagtcgtgt acttccgggt atgcaggcgg cttttgtaga tattgggctg
    12481 gataaagccg cgtttcttca tgcatctgac atcatgccgc acaccgaatg tgtggcgggt
    12541 gaagaacaaa agcaattcac ggtgcgcgac atctcggaac tggttcgtca ggggcaagat
    12601 ctgatggtgc aggtggtgaa agatccgctt ggcactaaag gtgcgcgcct gaccaccgat
    12661 atcacgctgc cttctcgcta tctggtgttt atgccagggg cttctcacgt tggggtttcc
    12721 caacgtattg aaagcgaatc agaacgtgaa cgcctgaaaa aagtggtcgc agagtattgc
    12781 gacgagcagg gcgggtttat catccgtacc gcagcggaag gggttggcga ggctgaactg
    12841 gcctccgatg ccgcttatct gaaacgcgtc tggaccaaag ttatggagcg taaaaaacgc
    12901 ccgcagaccc gttatcagct gtacggcgaa ctggcgctgg cgcagcgtgt tctgcgtgat
    12961 ttcgccgacg ccgaactgga ccgcattcgc gttgactcac gcctgactta cgaagcattg
    13021 ctggagttta cctcggagta cattcccgag atgaccagca agctggagca ttacaccgga
    13081 cgccagccga ttttcgatct ctttgatgtc gaaaacgaaa tccagcgagc gctggaacgc
    13141 aaagtagaac tgaaatccgg tggttatctg attatcgacc agaccgaagc gatgaccacc
    13201 gtggacatca ataccggagc gtttgtcggt catcgcaatc tggacgacac cattttcaat
    13261 accaatattg aagcgacgca ggctatcgct cgccagttac ggttgcgtaa tctgggcggg
    13321 attatcatta ttgatttcat cgatatgaat aatgaagatc accgccgccg cgtgctgcac
    13381 tcgctggagc aggcgttgag caaagaccgg gtgaaaacca gcgttaatgg tttttcggcg
    13441 ctggggctgg tggagatgac gcgtaaacgc acccgcgaaa gcattgagca cgtactgtgt
    13501 aacgaatgcc caacctgcca cggtcgcgga acggtgaaaa ccgtggaaac ggtatgctat
    13561 gaaatcatgc gcgagattgt tcgtgtccac catgcttacg attccgaccg tttcctggtc
    13621 tatgcttctc cggcagtagc cgaagccttg aaaggcgaag agtcacactc gctggcggaa
    13681 gtggaaattt tcgttggcaa acaggttaaa gtacaaattg aaccgctcta taaccaggag
    13741 cagtttgacg tcgtaatgat gtaaacagat gctgggccgc catccggcaa agggtttttg
    13801 agtcacattt ttagcagaca aggagtgacg ggtgaggcga ttgccgggga ttttactgct
    13861 tactggagcc gcgctcgttg tgatcgctgc cctgctggtt agcggcctgc gtattgcttt
    13921 accgcatctt gacgcctggc gtccggaaat cctcaacaaa atagaatccg cgactggcat
    13981 gccggttgaa gccagtcagc tctcagccag ctggcagaat tttggcccga cgcttgaagc
    14041 acacgacatc cgtgcagaac taaaagatgg cggcgaattt tcggttaaac gcgttactct
    14101 ggcgctggat gtctggcaga gcctgttaca tatgcgctgg cagtttcgcg atctcacttt
    14161 ctggcagctg cgctttcgca ccaacactcc tatcaccagc ggtggtagcg atgacagtct
    14221 ggaagccagc cacatcagcg atctgtttct tcgtcaattt gaccatttcg atctccgcga
    14281 cagtgaagtc agtttcctga cgccatccgg tcagcgcgcc gagctggcga tcccacaact
    14341 cacctggctg aacgatccac gtcgacaccg tgcggaaggt ctggtaagcc tctccagcct
    14401 taccggacag cacggcgtaa tgcaggtgcg catggatttg cgcgatgatg agggcttgtt
    14461 aagcaatggt cgcgtctggc tccaggcgga tgacatcgac ctgaagccgt ggctcggtaa
    14521 atggatgcag gacaatattg cgctggaaac ggcgcagttc tcccttgaag gctggatgac
    14581 gatcgataaa ggcgatgtaa ccggtggcga cgtctggctg aaacagggcg gtgccagctg
    14641 gttgggcgag aagcaaacgc atacgctgtc ggtgaataat ctgaccgcgc atattacgcg
    14701 tgaaaatccg ggctggcagt tctctattcc cgatacacgg atcacgatgg acggcaaacc
    14761 ctggccgagc ggagcattga cgctagcctg gataccggaa caggacgttg gcggcaaaga
    14821 caataaacgc agtgacgaac tccggattcg cgccagtaat ctggagctgg caggcctgga
    14881 gggcatacgc ccgctggccg cgaaactttc acctgcactg ggtgatgttt ggcgctccac
    14941 acaaccgagc ggcaagatta acactctggc gctggatatc ccgcttcagg cggcagacaa
    15001 gacccgtttt caggcatcgt ggagcgatct ggcctggaag caatggaaat tattaccggg
    15061 tgcggaacac ttctccggga cgctttccgg cagcgttgaa aatggtttgc ttaccgcgtc
    15121 gatgaagcag gcaaagatgc cttacgaaac ggtattccgt gcgccactgg aaatcgccga
    15181 cggccaggca actataagct ggctgaacaa tgacaaaggt ttccagctgg atgggagtga
    15241 tattgacgtt aaagccaaag ccgtccatgc gcgcggcggt tttcgttacc tgcaacctgc
    15301 taacgatgaa ccctggctgg gtattctggc tggcatcagt accgatgatg gttcacaagc
    15361 ctggcgctat ttcccggaaa acttgatggg taaagacctg gttgattact taagtggcgc
    15421 gattcagggc ggtgaagcgg ataacgcgac gctggtttat ggtggcaatc cgcaactctt
    15481 cccctataaa cacaacgaag gtcagtttga agtgctggtg ccgctgcgca acgcgaagtt
    15541 tgccttccag ccggactggc ctgcattaac taaccttgat attgaactgg actttattaa
    15601 cgacggttta tggatgaaaa ccgatggcgt taatctgggc ggcgtgcgcg cgagtaatct
    15661 caccgcagtg atccctgact actcgaaaga aaaactgctg attgacgctg acattaaagg
    15721 tccgggtaaa gccgttggcc cttactttga tgagacaccg ctgaaagatt ctctgggggc
    15781 gaccctgcaa gaactccagc tcgacggcga tgtgaatgct cgcttacatc ttgatatccc
    15841 gctgaacggc gaactggtaa ccgcgaaagg tgaagtgacg ctgcgtaata acagtctgtt
    15901 tatcaaacca ctcgacagca ccctgaaaaa tttgagcggt aaattcagct ttatcaatag
    15961 cgatctgcaa agtgaaccac tgacagcaag ctggtttaat cagccgttga acgtggattt
    16021 ttccaccaaa gaaggggcaa aagcctacca ggtagcggta aacctcaacg gtaactggca
    16081 accggcgaaa accggcgttc tgcctgaagc ggtgaacgaa gcattgagtg gcagcgtggc
    16141 gtgggatggt aaagtgggca ttgatctgcc ttatcatgct ggtgcgacct ataacataga
    16201 gctgaacggc gatctaaaga atgtgagcag tcacttacct tcaccgttag ccaaacctgc
    16261 gggtgaacca ctagcggtaa acgttaaggt tgatggcaat ctcaacagct ttgaattaac
    16321 cggacaggct ggtgcggata atcatttcaa tagccgctgg ttgctcggtc aaaagctgac
    16381 gctcgatcgt gctatttggg cggcagacag taaaacgctc ccgccgttgc cggaacaaag
    16441 tggcgttgaa ctcaatatgc cgccgatgaa tggtgccgag tggctggccc tgttccagaa
    16501 aggcgctgcg gagagtgtcg gtggtgcagc gagtttccca caacacataa cgttacgtac
    16561 gcctatgttg tcactgggaa atcagcaatg gaataacctg agtattgttt cgcaaccgac
    16621 ggcaaatggc accctggttg aagcgcaagg gcgtgaaatc aacgccacgc tagcgatgcg
    16681 taataacgcg ccgtggctgg cgaatatcaa atatctttat tacaacccga gcgtggcgaa
    16741 aactcgtggt gattcaacgc cgtcatcacc tttcccgaca acggagcgca ttaacttccg
    16801 tggctggccg gacgcccaaa tacgatgcac agagtgctgg ttctgggggc aaaaattcgg
    16861 tcgcattgac agtgatatca ccatttctgg cgatacgtta acgctgacca atggactgat
    16921 tgatactggt ttctcgcggc ttactgccga tggtgaatgg gttaataatc cggggaatga
    16981 acgtacctcg ctgaaaggaa aactgcgcgg gcagaaaatt gatgccgccg cagaattttt
    17041 tggtgtcacg acgcccatac gccagtcgtc atttaatgtg gattacgatt tacactggcg
    17101 caaagcaccc tggcagccag atgaagcgac gttgaatggc atcattcata ctcaactggg
    17161 taaaggcgaa attactgaaa tcaataccgg acatgccggg caattgctgc gcttattgag
    17221 cgtagatgcc ctgatgcgta agctgcgttt tgatttcaga gacacttttg gcgaagggtt
    17281 ctattttgac tccattcgca gcaccgcgtg gattaaagac ggcgttatgc acaccgacga
    17341 cacgctggtg gatggcctgg aggcggatat cgccatgaaa gggtcggtaa atctggtacg
    17401 tcgcgacctg aatatggaag cggttgtcgc accagagatt tctgcgacgg tgggcgtggc
    17461 tgcggctttt gcggttaacc ccattgttgg cgcggcagtg tttgccgcca gtaaagtgct
    17521 ggggccgctg tggagcaaag tctccatttt gcgctatcac atttcgggtc cgctggacga
    17581 tccgcaaatc aacgaagtgt tgcgccaacc gcgtaaagaa aaagcgcaat gatttgacga
    17641 gggcgcgtaa ttgccccaat ctcataggat aatcgttgcc aaaggccaac gagccagaac
    17701 ataaccgtag gtcggatagg gcgttcacgc cgcatccggc agccgttgcc tgatgcgacg
    17761 ctgtgcgtct tatcaggcct acaaacggaa cataaccgta ggtcggataa ggcgtttacg
    17821 ccgcatccgg cagccgttgc ctgatgcgac gctgtgcgtc ttatcaggcc tacaaacgga
    17881 acataaccgt aggtcggata aggcgtttac gccgcatccg acagccgttg cctgatgcga
    17941 cgctgtgcgt cttatcaggc ctacaaaccg gaacataacc gtaggctgga tagggcgttc
    18001 acgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag tagcaaaaac
    18061 gatgagtctt aacctggtaa gtgaacaatt gctagcggcg aacggcctga aacatcagga
    18121 cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg atctctattt
    18181 tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag atggttctta
    18241 caacatcgat cagggcgttg gtgtgcgtgc aatcagcggt gaaaaaaccg gatttgctta
    18301 cgctgaccaa atcagcctgc tggcgctgga acagagtacg caagcggcgc gcaccatcgt
    18361 ccgtgatagt ggtgatggta aagtacagac gctgggcgcg gtagagcata gcccgttgta
    18421 tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata tcctgcgtcg
    18481 cgtcgataag gttgcccgcg aagcggacaa gcgcgtacag gaagtgactg ccagcctcag
    18541 tggtgtctat gaattaattt tggttgcggc caccgacggc acgctagcgg cggatgtccg
    18601 tccgctggtg cgtctttccg tgagcgttct cgtcgaagaa gatggcaaac gcgaacgcgg
    18661 tgccagtggc ggcggcggtc gttttggtta tgaattcttc cttgccgatc tcgacggcga
    18721 agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca atctttctgc
    18781 cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc cgggcgtgct
    18841 gttgcatgaa gcggttggtc acggtctgga aggcgacttc aaccgccgtg gcacttcagt
    18901 atttagtgga caggtcgggg agctggtggc ttcagaactg tgtaccgtgg ttgatgatgg
    18961 cacgatggtc gatcgccgag gttcggtggc gattgatgac gaaggtacgc caggccagta
    19021 caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac tcaacgcgcg
    19081 tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc atctgcccat
    19141 gccgcgtatg accaacacct atatgctgcc gggtaaatcg accccgcagg aaattattga
    19201 atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg atatcacctc
    19261 cggcaaattc gttttctcca cttcagaagc atatctgatt gaaaacggta aagtaacgaa
    19321 gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa accatgcagc agatttcgat
    19381 ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag aagggcaaag
    19441 tttgccggtt ggcgtgggcc agccaacgtt gaaagtcgat aacctgactg ttggcggtac
    19501 tgcgtaataa tttcactatt ttcagaagga taattaatct ttctacgtgc acgaacggtc
    19561 ccctcgcccc tttggggaga gggttagggt gaggggaacc cgttggcaca ggtttgaaca
    19621 acgtaacagt acaatatgaa ttacttctct ttcccgcgcc catgcatctc ctgaaacaat
    19681 ttaccgacct caacaaaata atccgtcagc gagttgatca cgacctgtac cttcagcggc
    19741 agtttatctt tttcggtata caacgcataa accgggcgtg gatctgactg gtaacgcggc
    19801 agcaggatct ccagctcccc acgattgatc tcgttgatca cccacatcag cggcacgtag
    19861 gcgatcccgg cacccgccgt cagccagcgc accagcgtca tcggatcatt agtcacaaat
    19921 cttccttgtg ggatcaggcg agtcgagatc ccttccggtg cgatcagttc aaattcattg
    19981 tcgggccgca cgctgtattc aagccatgaa tgactactca aatcggcggg tttttccggt
    20041 atgccgtatt gtgtgagata gcttttcgcg gcgcacacca ccatcggcat cgcgcccaga
    20101 cggcgggaaa acaggctgga atcctgcaac gcgccgacgc ggattaccac atccagacca
    20161 tcggcaatca ggtcgggggc aggaattccg gtaaccagat tgacgctcaa acctgggtat
    20221 tctttcagca tttttgctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag
    20281 ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc
    20341 tgcacttcat ggagcatccg acggcagccc tggtagtaaa ttctgccggc ttcagtcaga
    20401 ccaatgctgc gtgtactacg gtttaacagc tttacctgca actcatcttc cagttttgac
    20461 accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag
    20521 ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct
    20581 gacttaaaag tgatttagat cacataatat agataacagc ataacagtta cgctaatata
    20641 ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct tctctgcggc
    20701 gacagatgct gaaaataata acgcctgctc tctctataca accaaggtcg acatgagtct
    20761 gtttcccgtt atcgtggtgt ttgggctgtc cttcccaccg atattttttg aattgctttt
    20821 atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt
    20881 tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc
    20941 gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca
    21001 tcacggtcgt attagtcatt ctggccttca tcgcaatttt taatgcctgg gtctattaca
    21061 ccgaatcccc gtggacgcgt gacgcgcgct ttagcgctga tgtcgttgcg atcgcgccgg
    21121 acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac
    21181 aggtactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg
    21241 atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaatc
    21301 gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggccaacaac gtactacaaa
    21361 cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg
    21421 aacgcacggt gatccgcgcg ccagcggatg gctgggtgac caacctcaac gtctataccg
    21481 gtgagtttat tactcgtgga tcaacggcgg ttgcgctggt gaaacagaac tctttctatg
    21541 tactggccta tatggaagaa actaagctgg aaggggtgcg ccctgggtat cgtgcagaga
    21601 tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgctgggg
    21661 tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gaccatcgac tctaaccttg
    21721 aatgggtgcg tttggcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga
    21781 acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg
    21841 aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac
    21901 gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc
    21961 cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt
    22021 actgacagcg gcgattgttg ctgccggtcc ggcctttgct gcgggaggtg aaccgtattc
    22081 tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc
    22141 cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg
    22201 tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtatgc
    22261 gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc ctgaaccatt
    22321 gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc
    22381 aattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtgg atcgagagct
    22441 ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg
    22501 tgaagttgtc gataaagcct ggggcgacct ggttcgacgc accacggcgc tacaaggtat
    22561 gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc
    22621 gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa
    22681 tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacac cggtagaaac
    22741 cgcgcaggac gtccacaagc agctcaaacg cctgcgaaga gttatcgcct ggaccgggga
    22801 acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct
    22861 caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg
    22921 cgaaccggaa gtaaaagtag agtcagccga acgtcatcat gcaatggtta acttctggcg
    22981 aaccacactt tcctgcattc tgggaacgct tttctggctg tggacgggct ggacttccgg
    23041 tagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc
    23101 acgcatggtg gcgatcgact ttatctacgg gacgctggcc gcgctgccgt tagggctgct
    23161 ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct
    23221 ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg gctcgatggg
    23281 ggcactggcc agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag
    23341 tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat
    23401 tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta atcagtttgt
    23461 ttctgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc
    23521 ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt
    23581 tcgcctggcg ctgacgatga ttatcgccca ccagcgcctg cgtgatgcgc cgatcccggt
    23641 taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc
    23701 tgcccgtagc gatgataaac gtcgtcggta ctttggtcag ttgctggaag aactggaaat
    23761 ctaccaggaa aagctacgca tctggcaagc tccaccgcag gtgacggaac cggttcatcg
    23821 gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga
    23881 cgccaaaagc gtcggttttt tcatggctat acttagcgat gcacggcaga actcgccgcg
    23941 aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc
    24001 aggaggattt ttatcgtgac tttagccaaa cctttggtct ggcgaaagat aaggtacgcg
    24061 atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg
    24121 aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg
    24181 atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa
    24241 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    24301 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    24361 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
    24421 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag catcaatgga
    24481 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag caacagtggt
    24541 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgacc
    24601 catatcacaa aatcaagtat acactaatta ttgagctaat taata
//
LOCUS       DAJOSY010000067        24555 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJOSY010000067 DAJOSY010000000
VERSION     DAJOSY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.067"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            214..717
                     /locus_tag="OHN27_002787"
     CDS             214..717
                     /locus_tag="OHN27_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCQ2287195.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(796..1047)
                     /locus_tag="OHN27_002788"
     CDS             complement(796..1047)
                     /locus_tag="OHN27_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCQ2287196.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1162..1248)
                     /gene="azuC"
                     /locus_tag="OHN27_002789"
     CDS             complement(1162..1248)
                     /gene="azuC"
                     /locus_tag="OHN27_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCQ2287197.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1511..1834
                     /locus_tag="OHN27_002790"
     CDS             1511..1834
                     /locus_tag="OHN27_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCQ2287198.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            2006..2503
                     /gene="ftnA"
                     /locus_tag="OHN27_002791"
     CDS             2006..2503
                     /gene="ftnA"
                     /locus_tag="OHN27_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCQ2287199.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2541..2780)
                     /locus_tag="OHN27_002792"
     CDS             complement(2541..2780)
                     /locus_tag="OHN27_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCQ2287200.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEDGFSTDQSKICRH"
     gene            2972..4183
                     /gene="tyrP"
                     /locus_tag="OHN27_002793"
     CDS             2972..4183
                     /gene="tyrP"
                     /locus_tag="OHN27_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCQ2287201.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4245..4910)
                     /gene="yecA"
                     /locus_tag="OHN27_002794"
     CDS             complement(4245..4910)
                     /gene="yecA"
                     /locus_tag="OHN27_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCQ2287202.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5106..5192)
                     /locus_tag="OHN27_002795"
     tRNA            complement(5106..5192)
                     /locus_tag="OHN27_002795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5156..5158),aa:Leu,seq:taa)
     gene            complement(5205..5278)
                     /locus_tag="OHN27_002796"
     tRNA            complement(5205..5278)
                     /locus_tag="OHN27_002796"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5244..5246),aa:Cys,seq:gca)
     gene            complement(5333..5408)
                     /locus_tag="OHN27_002797"
     tRNA            complement(5333..5408)
                     /locus_tag="OHN27_002797"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5373..5375),aa:Gly,seq:gcc)
     gene            complement(5560..6108)
                     /gene="pgsA"
                     /locus_tag="OHN27_002798"
     CDS             complement(5560..6108)
                     /gene="pgsA"
                     /locus_tag="OHN27_002798"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCQ2287203.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6165..7997)
                     /gene="uvrC"
                     /locus_tag="OHN27_002799"
     CDS             complement(6165..7997)
                     /gene="uvrC"
                     /locus_tag="OHN27_002799"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCQ2287204.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7994..8650)
                     /gene="uvrY"
                     /locus_tag="OHN27_002800"
     CDS             complement(7994..8650)
                     /gene="uvrY"
                     /locus_tag="OHN27_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCQ2287205.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8946..9122
                     /locus_tag="OHN27_002801"
     CDS             8946..9122
                     /locus_tag="OHN27_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287206.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9109..9333
                     /locus_tag="OHN27_002802"
     CDS             9109..9333
                     /locus_tag="OHN27_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCQ2287207.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9400..10122)
                     /gene="sdiA"
                     /locus_tag="OHN27_002803"
     CDS             complement(9400..10122)
                     /gene="sdiA"
                     /locus_tag="OHN27_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCQ2287208.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(10352..11104)
                     /gene="yecC"
                     /locus_tag="OHN27_002804"
     CDS             complement(10352..11104)
                     /gene="yecC"
                     /locus_tag="OHN27_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCQ2287209.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(11101..11769)
                     /gene="tcyL"
                     /locus_tag="OHN27_002805"
     CDS             complement(11101..11769)
                     /gene="tcyL"
                     /locus_tag="OHN27_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCQ2287210.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11784..12770)
                     /gene="dcyD"
                     /locus_tag="OHN27_002806"
     CDS             complement(11784..12770)
                     /gene="dcyD"
                     /locus_tag="OHN27_002806"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCQ2287211.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(12875..13675)
                     /gene="tcyJ"
                     /locus_tag="OHN27_002807"
     CDS             complement(12875..13675)
                     /gene="tcyJ"
                     /locus_tag="OHN27_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2287212.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(13763..14314)
                     /gene="fliZ"
                     /locus_tag="OHN27_002808"
     CDS             complement(13763..14314)
                     /gene="fliZ"
                     /locus_tag="OHN27_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCQ2287213.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(14360..15079)
                     /gene="fliA"
                     /locus_tag="OHN27_002809"
     CDS             complement(14360..15079)
                     /gene="fliA"
                     /locus_tag="OHN27_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCQ2287214.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(15399..17111)
                     /locus_tag="OHN27_002810"
     CDS             complement(15399..17111)
                     /locus_tag="OHN27_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCQ2287215.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSTSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSSTLKLTSFNVNGKGAVDNAKATEADLTAAGFSQGAVVSGNSTWTKSTVTTFNA
                     ATATDVLASVSGGSTISGYTGTNNGLGVAASTAYTYNATSKSYSFDATALTNGDGTGA
                     TTKVADVLKAYAANGDNTAQISIGGSAQDVKIASDGTLTDVNGDALYIGSDGNLTKNQ
                     AGGPDAATLDGIFNGANGNAAVDAKITFGSGMTVDFTQASKKVDIKGATVSAEDMDTA
                     LTGQAYTVANGAQSFDVAAGGAVTATTGGATVNIGADGELTTATNKTVTETYHEFANG
                     NILDDDGAALYKAADGSLTTEATGKSEVTTDPLKALDDAIASVDKFRSSLGAVQNRLD
                     SAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLS
                     LLQG"
     gene            17377..18783
                     /gene="fliD"
                     /locus_tag="OHN27_002811"
     CDS             17377..18783
                     /gene="fliD"
                     /locus_tag="OHN27_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCQ2287216.1"
                     /translation="MASISSLGVGSGLDLSSILESLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            18808..19218
                     /gene="fliS"
                     /locus_tag="OHN27_002812"
     CDS             18808..19218
                     /gene="fliS"
                     /locus_tag="OHN27_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCQ2287217.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            19218..19583
                     /gene="fliT"
                     /locus_tag="OHN27_002813"
     CDS             19218..19583
                     /gene="fliT"
                     /locus_tag="OHN27_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCQ2287218.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEINPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFL"
     gene            19661..21148
                     /gene="amyA"
                     /locus_tag="OHN27_002814"
     CDS             19661..21148
                     /gene="amyA"
                     /locus_tag="OHN27_002814"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCQ2287219.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(21182..21595)
                     /locus_tag="OHN27_002815"
     CDS             complement(21182..21595)
                     /locus_tag="OHN27_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2287220.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            21782..22987
                     /gene="yedE"
                     /locus_tag="OHN27_002816"
     CDS             21782..22987
                     /gene="yedE"
                     /locus_tag="OHN27_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCQ2287221.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            22984..23217
                     /gene="yedF"
                     /locus_tag="OHN27_002817"
     CDS             22984..23217
                     /gene="yedF"
                     /locus_tag="OHN27_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCQ2287222.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPQDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            23324..23995
                     /locus_tag="OHN27_002818"
     CDS             23324..23995
                     /locus_tag="OHN27_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCQ2287223.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINAHVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(24035..24555)
                     /locus_tag="OHN27_002819"
                     /pseudo
     CDS             complement(24035..24555)
                     /locus_tag="OHN27_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6027 a         6280 c         5951 g         6297 t
ORIGIN      
        1 ttgatttcat caatgaaatg taaaaatata taaacttgat gatttaagca ttttcttata
       61 cccgttcaga cgttattctt atttcagatc atcgtcagaa ttgactccac gatcacattt
      121 cggaccggca gaaaggaatt attctgcaaa cagtaattat ggtgttttga tttatcttgc
      181 acctctccac ttctggatat aaggatatta ggtatggcaa ccgctggaat gcttctcaaa
      241 ctcaactctc aaatgaaccg cgagttttac gcatccaatc tctaccttca cctgagtaac
      301 tggtgttctg aacagagtct gaacggcacc gccactttcc ttcgcgccca ggcacagagt
      361 aatgtgaccc aaatgatgcg catgtttaac tttatgaaga gtgtcggcgc tacccccatc
      421 gttaaagcca ttgatgttcc cggtgaaaaa ctgaactctc tggaagaact gttccaaaaa
      481 acgatggaag aatacgagca acgttctagt acgttggcac agttagccga tgaagcgaaa
      541 gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc tggaaaaaga acagcagcat
      601 gatggtctgt tgctgcaaac cattcttgat gaagtgcgca gtgcgaaact tgcgggtatg
      661 tgccctgtgc agaccgacca acatgttctg aatgtcgtgt cacaccagct gcattgatca
      721 tcatcggcgc taatgcattg cgccgatgaa ggttttgaga aaccgctgcc tcatctgttt
      781 gaagcagcgg tttttttaat gggattcacc ctgtggggta aacttgagtt caataagcgc
      841 gatggctttt tggattgccc gcatggtgac cgggtctgcg gcggcgggat ggttagtaaa
      901 gtcgatattc ttcagctgac tggacatttt ttcacgaact tcaacgggcg cgattacatc
      961 gagaacatcc agaatttgtt tgataaccaa ctggcaagca accacatcag aaaccagttc
     1021 ctgatcggca ttcagcggct gggacatcgt aaactcctga tagcattttg aaagccgtta
     1081 tagtagcggc ttcacatctt cagcgatagt cacatccacc gtcatcagga cacaaaaaaa
     1141 cctgccggag caggtttttt gttatcggaa catattgcct ggcggtacgt ctttgaacgt
     1201 cttgcaatag ttattgaaca tacttttcag tattttgcgc agtttcatcg cggcactccg
     1261 accatttgtt atacaggtgt tattgtcttt gcacttataa tatgacaacc atcacaaaaa
     1321 tcaatcttta tgtgatgtga atcacataaa taccccttta atgttataaa aatgataatc
     1381 aaaaagcagc taccccattt ctgacaccta cagatggcaa gaaatagcgc ctgccaggcg
     1441 tcttttccgg ccattgtcgc agcgctgtaa cgcgtaaaat agtgctttct tttactcttc
     1501 tggctggacc atgagacttc tgattctgac tctttcatta ataacgctgg cggggtgtac
     1561 ggttactcgc caggcccatg tgagcgaagt tgacgcggca acaggtattg tacggttggt
     1621 ttatgaccag gcttttttgc agcatgcccg taccgatcgt tatgtcagtc gcgggatcgc
     1681 cgatcgcgca tgccagcagg aaggctatac ccacgctatc ccctttggtc agccagtagg
     1741 caactgtagc ctttttgcgg gttccttatg tttaaatacg gaattcactt tgtcatatca
     1801 gtgccatcat tccgccttcc ctgtctttct ctaaatgttt taatcagcga gggggatctt
     1861 cgctgattaa ataaatagcg gtaatgcgtt ttattcccat tcgtattttt aataaatgaa
     1921 gtttatattt taccttttgc aaataataaa ataacaaatt atagtgacgc cacagcaaca
     1981 aatataacct ttgtggagca ctatcatgct gaaaccagaa atgattgaaa aacttaatga
     2041 gcagatgaac ctggaactgt actcttcact gctttatcag caaatgagcg cctggtgcag
     2101 ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgc cacgcccagg aagagatgac
     2161 acatatgcag cgtctgtttg attacctgac tgataccgga aacttaccac gtattaatac
     2221 cgttgaatct ccgtttgctg aatattcttc acttgatgaa ttattccagg aaacctataa
     2281 acacgaacaa ttaatcacgc agaaaattaa cgaactggct catgctgcaa tgaccaatca
     2341 ggactaccca acatttaatt tcctgcaatg gtatgtttct gagcagcatg aagaagagaa
     2401 actgttcaaa tcgattattg ataaattaag cctggcaggc aaaagcggcg aaggtctgta
     2461 ttttatcgac aaagaactct ctaccctcga cacacaaaac taatgctctc cggcggcagg
     2521 ttttctgccg ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt cgaaaaaccg
     2581 tcttcagaag gaataaattt gccttttgct gccagaaacg ccaccagctc tcccgctgtc
     2641 atcccttctg ccgagcaggt gtgaaaacgt gcctgttcac caaaacgcgc tttaatcgca
     2701 gcttccagac tggcatgcgt atattgctcg cctgattcaa tcatcatatt taacacttca
     2761 tgaccgtgaa tagagtccat cgttcctcct caaaaaaaag cctagcgtag cgattgccgc
     2821 ttatgaagac tttgcgccag cgcaggactg aatgcttttt attgtacatt tatatttaca
     2881 ccatatgtaa cgtcggtttg acgaagcagc cgttatgcct taacctgcgc cgcagatatc
     2941 actcataaag atcgtcagga cagaagaaag cgtgaaaaac agaaccctgg gaagtgtttt
     3001 tatcgtggcg ggaaccacaa ttggcgcagg catgctggca atgccgctgg ctgcggcagg
     3061 tgttggtttt agcgtcacgt taatcttgtt gattgggctt tgggcgttga tgtgctacac
     3121 ggcgctatta ctgctggagg tgtaccagca tgttccggca gataccggct tgggcacgct
     3181 ggcaaaacgc tatctgggac gctacggtca atggctgacg ggcttcagta tgatgttctt
     3241 aatgtatgct ctgactgcgg catacatcag cggtgctggt gaattgttgg cctccagcat
     3301 cagcgactgg acaggtattt caatgtcggc aaccgctggc gtgctgttgt tcacttttgt
     3361 tgccggtggc gtggtttgtg tcggaacttc gctggtcgat ttgtttaacc gttttctgtt
     3421 cagcgccaag attatttttc tggtggtaat gctggtacta ctgctaccgc atattcacaa
     3481 agtgaatctt ttaaccctgc cgttgcaaca ggggctggca ctatctgcaa tcccggtgat
     3541 ttttacgtcg tttggttttc acggtagcgt gccgagtatt gtcagctata tggatggcaa
     3601 cattcgtaag ctacgctggg tgtttataac cggtagtgcg atccccctgg tggcatatat
     3661 tttctggcag gtggcgacgc ttggcagcat tgattcaaca acctttatgg gattgctggc
     3721 taatcatgcc ggattaaacg ggctgttaca ggcgttacgc gaaatggtgg cctctccgca
     3781 tgttgagctg gcagtgcatt tatttgctga tttagccctc gccacgtcat ttctcggcgt
     3841 tgcgttaggc ttatttgatt atctggctga tttgtttcag cgttcaaata ccgttggtgg
     3901 acgattgcaa actggtgcaa ttacctttct gccgccgttg gcgtttgcac tgttttatcc
     3961 acgaggattt gtgatggcgc tgggttacgc cggtgtggcg ctggcggtac tggcattgat
     4021 tatcccttca ctattgacct ggcaaagcag aaagcacaat cctcaggcgg gttaccgggt
     4081 gaaaggtggt cgtccggcgc tggtggtggt gtttctctgt ggtattgctg tgattggcgt
     4141 gcaatttttg attgcggcag ggttgttacc agaagtgggg tgatcagata gcctcaaatt
     4201 ccttattggg tgccagaatt aacgctgaca cccaatttga cttcttaatg caggcagcac
     4261 tgcttaaatt tcttaccact gccgcacgga caaggatcgt tacgccccgg tttctcttct
     4321 gctttgatcg gttgctgaac agctttttcc tgcggatgcg ccatccagta tgcatgtaaa
     4381 tcaagcgccg ccagtcgaat ggcatctacg ctctcttcaa acgcttctgg cgacatcttc
     4441 tctacccgct cgaagttttc ctcagtaccg tgcagcgcta tcgcctccag cgctggtttt
     4501 aacgaatcgg gcaatgttga ccagtcagaa agtgccacgc cccgcatata gccaaagcac
     4561 cactcctcaa caatcgtcag ctcgctgcca tcaacttctc gcaagccgaa tagcggctca
     4621 aactgctccg ggaattcgtt cagacgctct gcggtatcgg ccatatgttg aaaggccagg
     4681 ttcataaagc gtgtcatctc tttctcagac gcccagcgcg gcacatagtc agccccaccc
     4741 cacacggcaa ccagccactg tgccggttca atctcttgcg gagaactcaa caccgccgtc
     4801 aataaaccgt ccagctccgc cacatcaagg atggcgtggt cagtgttgta tttggtcaga
     4861 atatcgtcca gccattccaa ctcactttcg tttaacggtc ccgttttcat acgcttttcc
     4921 ttgtggatct caactcgcca gcacctatct tacatgccgg tccgtatcag agatactttt
     4981 tgagtggctt tgccggtgat taaaaattaa ggagggtgta acgacaagtt gcaggcacaa
     5041 aaaaaccacc cgaaggtggt ttcacgacac tgcttattgc tttgatttta ttcttatctt
     5101 tcccatggta cccggagcgg gacttgaacc cgcacagcgc gaacgccgag ggattttaaa
     5161 tcccttgtgt ctaccgattc caccatccgg gctcgggaag aaagtggagg cgcgttccgg
     5221 agtcgaaccg gactagacgg atttgcaatc cgctacataa ccgctttgtt aacgcgccaa
     5281 attcttcagg cctttcagcc agacatccgc ttgaggccga tgtcttttaa actggagcgg
     5341 gaaacgagac tcgaactcgc gaccccgacc ttggcaaggt cgtgctctac caactgagct
     5401 attcccgcat tcatcaagca atcagttaat cacttgattt tattatcgtc tggcaatcag
     5461 tgccgccgtt cgatgcgttg cattctactt acctggcgcg atgagtcaac gatatttttc
     5521 accacttttg atcgtttgct gaaaattgca ccgaaacgat cactgatcaa gcaaatctgc
     5581 acgcgcagcg ctcaaatatt gcaacattga ccacagagtc agtaccgcag ccacaaagaa
     5641 aagtgcaata ccggcgtact caacccaaat gttcggacgc cacagcagcc aggccaacgc
     5701 caccatctgg gccgtagttt tcactttccc aatccaggag acagccacgc tactgcgttt
     5761 acccaactcc gccatccatt cgcgtagcgc agaaataata atttcacggg cgatcatcgt
     5821 tgccgccggt aaggtcaccc accagctgtg ataatgctcg gttaccagca ccatggcgat
     5881 agccacgaga actttatctg cgacagggtc aaggaaagca ccaaaccggg tactctggtt
     5941 ccagcggcgt gccagaaaac catcgaacca gtcagtcacc gccgcgacgc agaaaatgag
     6001 cgcggcggca aacggcgacc aggtgacagg cagataaaag accaatacaa agaatgggat
     6061 aaggatgaca cggaacagtg taagcaacgt agggatatta aattgcataa tgacgggtaa
     6121 ctatctgttg tcagtaagat tacccctatg ttgctacaga gacatcaatg tttcaacgac
     6181 cagaagatct tttctgccag accttgcgaa atacccggca cttttgcaat ttcctcgacg
     6241 ctggcgttac gtaaaccttg caaaccgccc atatatttca acaacatttg ccgacgtttt
     6301 ggcccgacgc cttcaatggt ttccagggaa ctggtatttt tgaccttcgc ccgtttttta
     6361 cggtgcccgc caatcgcgtg atcgtgtgat tcatcgcgaa tatgctggat aacatgcagc
     6421 gcgggagaat ctggcggcaa actgaatccc tcaccttccg gctcaaagaa cagcgtttcc
     6481 agcccagcct tacgatctgc tcctttggca acgccaagta gcagcggatg atttttatcc
     6541 catgagacat ccagttcggc gaagacattt ttcgcctgcg caagctggcc tttgccgccg
     6601 tcgataagga tcacatccgg gatcttactg tcgtcaatgg ctttaccata acgccgacgc
     6661 agcacctgat tcatcgccgc ataatcatcg cccggcgtga tgccagtaat gttatagcgc
     6721 cgatactccg cacgcagcgg gccgttagca tcaaacacca cacaggaagc gacggtttgt
     6781 tcgcccatgg tatggctgat gtcaaagcac tccatccgct tcacttccgg caatttcaac
     6841 acgctggcaa gcgcggtcag tcgctggtga acggtagatt gctgcgaaag tttgctggtt
     6901 aaggccgtcg ccgcattggt gcgcgcgagt ttcagataac gcgccctatc gccgcgaggt
     6961 ttggtttgaa cattaatctt gcgtcccgcc agttctgaaa gggaatcggc gagcagcgtt
     7021 ttatcgctaa gattaaaatc gagcaggatc tcacccggta aggtgcgcat ctggctgcct
     7081 tgtaaataga actgacctac gaaggtttcc accacctcgc tcagttccgt accgccaggc
     7141 actttcggga aatagctgcg gctgccgagc actttgccct gacgaatgaa caatacgtgg
     7201 acacaagcca tgcccgcatc gaacgccaca ccaataacgt caaggtcgtc gccggtattg
     7261 gaaacaaact gtttttcggt gacgcgtcgc accgcctgaa tttggtcgcg aatacgtgca
     7321 gcttcttcaa actccagatt ctggctggca gtttccatac ggctaatcag ttgcgtaagc
     7381 acctgatcat ctttgccaga caaaaacagg cgcacatact cgacctgctg agcgtattct
     7441 tcttcactca ccagtccttc aacgcacggc cccagacaac gtcctatctg atattgcaga
     7501 cacggacgcg agcgattgcg ataaacactg ttttcgcact ggcgaatggg gaaaatcttt
     7561 tgcagtagcg ccagtgtttc acgtacggca tagccattcg ggaacgggcc gaaatattca
     7621 cctttagcat gcttcgcacc acgatgcatc gccagacgcg gatgggtatc gccactcagg
     7681 aagataaagg gataagattt atcatcgcgt agtaaaacgt tgtaacgcgg ctgatagagt
     7741 ttgatgtagt tgtgttccag caacagcgct tcggtttctg tatgagtaac cgttacatca
     7801 atttgctgga tctgggcgac cagcgcttcg gttttgcgcg aagcgaggtt gctacggaaa
     7861 tagctggaaa gccgtttttt caggtctttc gctttgccga catagataac cgtaccacca
     7921 gcatcgtaca tgcgataaac gcctggctgg ctggttacgg tttttaaaaa cgcttttgcg
     7981 tcaaactgat cactcactga cttgataatg tctccgcatt acacagacca tggcgaattg
     8041 ccaggtgagt cagctcaaca tcgccatgaa tgtttagttt actgaacata cgatagcggt
     8101 agctgttcac cgttttcgga ctgagattga gctgttctga gatctcattg accttctggc
     8161 ccttggtgat catcagcata atctgcaatt cacgttcaga caaactggca aatgggcttt
     8221 ctgttttttc tggttcgatc tggcttaacg ccatttgttg agcgatatca gaagcaatgt
     8281 aacgctgccc tgaatagaca gaacgaatcg cactcacgac ttcctgcgga gccgcgcctt
     8341 tgctgaggta gcccgcagca ccggcctgca tgactttcgc tggtaaaggg ttttctgtat
     8401 ggacggtaag catgatgatt ttgacatcag ctgtggaacg cgcgatttta cgcgtcgcct
     8461 caagaccgcc aatgcccggc atactcatgt ccattagcac cacgtcaacg gcatttgtcc
     8521 ggcaccactt aacggcgtct tcaccgcacg atgcctcacc gacgactttt atacccttta
     8581 tatcttccag aatgcgtcgt atccctgcgc gcaccagttc gtggtcatca acaagtagaa
     8641 cgttgatcaa aggaatatct ccagaaatag ggataacgct actgatagtt agtcattcgt
     8701 atattagcgg tttttattgc aactttgaaa cgttaaaaat ggtgggtatt cgatttttct
     8761 ctcgtttttg tcaattcgat tgtccacagt tatggtcacg cccgtcacca acggcttacc
     8821 ggatcgtttc acagtaagcc ctacgatgaa atgtgacaaa aattgacttt attcagcaaa
     8881 aatgaaaatc agcccgctga atatgtaaca ttaattaacc cgaacaatac gggcaaaaaa
     8941 cattcatgat aaaaatattt attggtcatt atattaacgt attttatagc actgccgata
     9001 tcacgctcaa aaaacaacca ctgctatttt tagcaaagct tatggtatac tccgccgcct
     9061 taacattttt caccgcaaat tttcattgca acatgacgag gaaaataaat gagtacgcct
     9121 gatttttcta ctgccgagaa taatcaagaa ctggcaaatg aagtctcctg cctgaaagcg
     9181 atgctgacgc tgatgctgca ggcgatggga caagctgacg cgggccgcgt gatgttaaag
     9241 atggaaaaac agcttgcgct gatcgaagac gaaacccagg ctgcagtatt ttccaaaacg
     9301 gttaagcaaa ttaaacaagc ctaccgtcag taatataaaa ccggctgaga gcgtgccttt
     9361 cagccggttt ttgcatctgg cacgcagaaa gaaaagcgat caaattaagc cagtagcggc
     9421 cgcgtaacag gccacctggg tcttatttgg tgcattgatt tttttctgca tgtttttctg
     9481 atggaaattg accgtattct cagagattga caaaatcatc gctatctctg ctgatgtctt
     9541 cccttccgcc gtccatttca gaatttcttt ttcgcgcttg ctgaaattca tctctggcgt
     9601 catcactatt tcatcattta aacgcatcag agccatcaga ctttcgcgca ccagtaactg
     9661 catttttaat tgcagttcat cactaagaat gggtatttcg cgcgtgctgc aacgggaaaa
     9721 ggacaaaaag cccagcgccc gatttggcag cattaaatac tgagtgacac cgcggcgtaa
     9781 accatgtgcg tgcgcggctt cccataatgg ctgtgcttcg ctgaataagt catcattcca
     9841 cattaaatgg ccctgactaa agttttcagg gttcagcaca ggatcaattg cgagaaaatt
     9901 ttttgcctga taataactaa cccacgactc agggtaattg gtgtagaaag ccactttagg
     9961 tcgcgtgaat ggtaccgggt ggcgaacaca taacgaatag taatcgtact ccagctgctg
    10021 agcctgaagc tctatttcat ggtagacctc ttctgcggcc tccatcctct gaaaacgcaa
    10081 cagcatcgtg cgacgccagc tgaaaaaatc cgtatcctgc atagtaaacc gcaacgcccc
    10141 tgagagtgag tatcatttat aatgataatt caaactatca cataaataag atttatatat
    10201 aatttatatt attcaggcaa tgaattactt ttgcaagcca tcgcattctc ttatgttatt
    10261 aatgagttat gcagatttgt taagcagttt tatcacgctt gaaatggcgt cccgccccga
    10321 caggtgaatc gtcggggctg attttttctt attattgcag cagaaacttc tcgaggaact
    10381 gtcgggtgcg aggctgctgg ggatcggcaa ataacgcttt tgctgccccc tgctcgacta
    10441 tccgcccctg gtccataaaa atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt
    10501 gcgtcacaat caccatcgtg cgcttttcct gcgccagctg gcggattgtg ttcaggactt
    10561 cgcccaccag ctctggatcc agcgctgacg ttggctcgtc aaacaaaatc acctcaggac
    10621 gcattgccag cgcacgcgca atcgcaacac gctgctgttg accgccagac aaacgacgtg
    10681 gatagctggt ttctttaccg gccagcccaa ctttcgccag cagctcgcga gcgcgcgccg
    10741 tggcctcttc tttcggttcg cctttgacga tcaccggccc ttcaataatg ttttccagca
    10801 ccgtacgatg cggaaacaaa ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact
    10861 ggcgaatcag cgatttttgc tgacttaatg aacgtgcagt atcaatagtg atatcgccaa
    10921 cggtgatcgt tcccgcttcc ggttgttcca gcagatttat gctgcgtagc aacgtggttt
    10981 tgccggaacc actcggaccg ataattgcca ccacttcgcc aggctttacc tcaaggtcga
    11041 taccgtgcag caccgtctga ccgtgaaatt ttttcaccag gttcttaact tcaatggcac
    11101 tcattttggt tctctctcct ggcgattaag ttgattctca aaatggttct gcaacgtcga
    11161 taatactgtc gccatgatcc agtagatgag cgaagccgcc aggtacatgg tgaacacctc
    11221 cagcgtgcgc gaggtaatca actgcgcttg acggaacagc tccggcacct ggattgtcgc
    11281 ggccagcgac gtatctttta ccaggctgat aaagctgttc gacagcggcg gcaacgccac
    11341 ccgcgccgcc tgtggcaaaa tcgcgcgacg catagtctgc catggcgtca taccaatact
    11401 ggcagctgct tcccactgac ctttatcaat agaagaaatg gcggcgcgaa gcgtttcggc
    11461 agcataggcg gcagtgttca gcgacagacc aatcattgct gacggaatgg gatctaattc
    11521 gataccaaac tgcggcaggc cgtaatagat cataaacagt tgtgcgatga gcggcgtacc
    11581 acgaaaaata gagatataaa aacgcgccag ccagcgcacg ggccagatcg gcgacaggcg
    11641 catcagcgcg agaataaacc cgagcagtaa gccaaaaaac atgccgccga tactgagttg
    11701 cagcgtatac ccagcccctt tcagcaggaa cggcaaggaa tcaataacca gttgtatact
    11761 ttcttgcatg agcgtttccg gagctaaacg tggggatgat aggcgaacag cgcaggcgcg
    11821 ccgccggtat gaataaacag aatcggccct tcatctttga aacgtttctg actgataccg
    11881 tcaatcagac ccgccatcgc ttttccggta tacacaggat caagcagaat gccttccagc
    11941 cgcgccagca atttcactgc ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca
    12001 aaatagtcat cccagagtaa aatttccgct gatgcggtca gctccagttc tttcgcaatc
    12061 gcctgttgta ggttaaccac tttcggcaat tgatcggcaa cggaacgcga cacggtcacg
    12121 ccaatcagtt cgctttcagg catcaggtgt tccagcccaa cagccagtcc ggcgtgagtt
    12181 ccggcactgc ccgatgcgac caccaccgac gaaatattaa ccgccccttc acactgttgc
    12241 gcaatttcca gcgcactctc cacataaccc agcgcgccca gagcattaga accgccaacc
    12301 ggaatgacat acgggcgaaa gccttgtgct tcgactcgcg tcgccagctc ttccagttgg
    12361 gcattgggat cggtcagtgc gtcgcacatt tcaatctggg tattgaacag atccagcaac
    12421 aaacgattgc cgttggttaa atagttttct gcggttgtgc caataggatt ttccagcagc
    12481 gccacgcagt gcagaccgag tttcgccgca actgcggcag tctggcgcac atggttagac
    12541 tggatcgccc cggcagtaat cagcgtatcg gcaccttcac gcagagcatc tgccgcgaga
    12601 aattccagct tacgtaattt attgccgccc attgccatgg gtgtgacgtc atcccgtttg
    12661 atgaaaattt cccgtccaag ataatcagaa aagcgcggca gatattcgag cggcgttggc
    12721 gcgccgataa actccagccg tggaaaacgg gttaaattat gcagtggcat aacagcctcc
    12781 gatgtgtgtt gttgtgattt tcttattatg cacgctgaaa acgcgtaaat aaaaaaggcg
    12841 ctagtgaaag cgcccttttt tgtcattatg ctgattattt ggtcacatca gcaccaaacc
    12901 atttttcgga aagggcttgc agagtgccat ctttttgcat ttccgcaatt gcatcattca
    12961 ctgctttcag caggtcttca tttcctttac gcagcgccac gccagactcc tgacgggaga
    13021 atgcttcacc ggttactgcc agcgtatcgt tggttttctt caccagatcc agcgccgcca
    13081 gacggtcaac gaggatcgca tcgatacggc ctacgcgcag atcctgatat ttggtcgggt
    13141 catcatcata ggtacgcaca tcgacgccct gaacattctg acgcagccac tcttcatagt
    13201 tggtgcccag gccgacaccc acttttttgc ctttcagatc agcagctgtt ttaatggtgc
    13261 cttcgttacc ttttttcacc agcgcctgaa taccggaaat ggtgtaaggg gtagagaaat
    13321 cgtatttttt cttgcgctcg tcagaaatgg tgacctgatt aatcaccaca tcaatgcgtt
    13381 tagagtccag cgacgccagc ataccgtccc atttggtcgg ttttagtgac gcctcaacgc
    13441 caagatgttt tgccagctgt tgggcaaatt ccacttcaaa accggttaat ttgccgtcat
    13501 ctccctgaaa actgaacggc ggataagttc cttccagccc taccagcagc gtgccgcgct
    13561 ctttaacttt attaagcaga ccttcatctg caaaactttt aacgctcatg cccgcaacca
    13621 gcgccacggc catcacaccc atcaatgcct gacgtcccag atgtgctaat ttcatattca
    13681 ccccgaatgt tgttatgtct gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa
    13741 ctccgctaca tcttattctt atttaatata tatcagaagc aggcaggctg gaggatttct
    13801 gcacaagtcc ggtacaggtt tggcgctgat agtgttgata cttccgtaac gcgatgcggt
    13861 aattgttggt gctggtggtg ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct
    13921 gcagctgatc gagggaaata ttttgctcgt gtagatgatt ccccaaacgg cgcagacgaa
    13981 cgacatattc acgcaccgta cctgggctca tttcagtttg ctcaaaaaga aattgcttaa
    14041 agccgataat atcgaaaaaa tcactctgcg ttttgcaatg taaatcaccg caaaatcggc
    14101 acaatgccgc ccatgatttt tgttccgttt gccagccctt ttcatcaagc agcgtgtcca
    14161 ggcgggaaat ctcgatttta ttcactattt tgccgtcgcg aacgagggta atgcgatcga
    14221 gtaatttacg gcaatgcgcg caatgggtct ggctgtgttt aaagtcttta agatagcggc
    14281 ttaatggccg tcttttcagg tgctgcacca tcattaagaa ctcctggtag tcaaagttaa
    14341 agtgcggcat ttactgacgt tataacttac ccagtttagt gcgtaaccgt ttaatagcct
    14401 ggctgtgtaa ctgactgacc cgcgattccc cgacctccag cactgcacca atctctttga
    14461 gattcagctc ttcctggtaa tagagggtta ataccagttt ttcgcgctcc ggcaacgttt
    14521 cgatggcttc catcacccgc tggcgcagat tattgtccag cagttgttgt agcgggtttt
    14581 ctcgctgatg atcatcagta accagttcga tgctatcgcc gtgctcttcg cgccactcat
    14641 cgtaggagaa gagctggctg ttattggtgt cgagcaacat ttggcgataa tcggcaatat
    14701 cgatccctaa acgttccgct acctcagttt ccgtggcgtt gcggccaagt tcctgctcca
    14761 gttgccctat tgcctgtgcc acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt
    14821 cacggctgcg aagttcatcc agcatagcgc cacggatacg ctgcactgcg taagttgtaa
    14881 atgccgttcc ttgtagggcg tcatagcgtt cgacggcatt aagtaaccca atgccgcccg
    14941 cctgtagcag atcgtcaagt tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt
    15001 cgtgacgcac cagcgggaca taacgctgcc acagcgagtg tttatccatt acaccttcag
    15061 cggtatagag tgaattcacg ataaacagcc ctgcgttata tgagttatcg gcatgattat
    15121 ccgtttctgc agggttttta atcggacgat tagtgggtga aatgaggggt tatttggggg
    15181 ttaccggtaa attgcggaca gaaaaaaccc cgccggtggc ggggaagcac gttgctgaca
    15241 aattaccatt catgttgccg gatgcggcgt aaacgcctta tccggcctac aaaaatgtgc
    15301 aaattcaata aattgcaatt caccttgtag gcctgataag cgcagcgcat caggcaattt
    15361 ggcgttgccg tcagtctcag ttaatcaggt tacgacgatt aaccctgcag cagagacaga
    15421 acctgctgcg gtacctggtt ggcttttgcc agcacggagt taccggcctg ctggatgatc
    15481 tgcgctttcg acatattgga cacttcggtc gcatagtcgg cgtcctgaat gcgggactgc
    15541 gcttcagaca ggttggtagt ggtgttgttc aggttggtga ctgcggaatc cagacggttc
    15601 tgcaccgcac cgagggagga gcggaatttg tctacggatg cgatagcatc gtccagcgct
    15661 ttcagcggat ccgtggtcac ttcggattta ccagtagctt cagtggtcag agaaccgtca
    15721 gccgctttgt acagagccgc gccgtcatca tccagaatat tgccgttagc aaattcgtga
    15781 taagtttctg tgacagtctt gttggtcgca gtcgtcagtt caccatcagc accaatattt
    15841 acggtagcgc cacctgtagt agcggttact gccccaccag cggcaacgtc aaaagactgt
    15901 gcgccgttag ctacggtata agcctgccca gttaacgcag tgtccatatc ttcggcggat
    15961 accgttgcgc ccttaatatc cacttttttg ctagcctggg tgaaatcaac ggtcatgccg
    16021 ctgccgaatg taatcttcgc atcaactgct gcattaccat tcgcaccgtt gaaaataccg
    16081 tccaacgttg ccgcatctgg accgccggcc tggtttttag tcaggttgcc gtcagaacca
    16141 atatataaag catcaccatt gacgtcagtc agggtgccat cgctggcaat tttaacgtcc
    16201 tgagcgcttc cgccgatgga gatctgagcc gtattatcac cgtttgctgc ataggctttc
    16261 agcacatcag caactttagt ggtggcccca gtaccatcgc cattggtaag tgcggttgcg
    16321 tcaaatgaat aagacttgct ggttgcgttg taggtatatg cagtagaagc cgctacgcct
    16381 aatccattgt ttgtaccggt ataaccgcta atagtgctgc cgccgctaac gcttgccagc
    16441 acgtcggtag ctgttgctgc attaaaggta gtaacagtag atttagtcca ggtgctgttg
    16501 ccactgacga ctgcaccttg ggagaagccc gcagcggtca gatctgcttc agtggcttta
    16561 gcattatcaa cagcgccttt accgttaaca ttgaagctgg tcagcttcaa tgtagaagag
    16621 tcgatctttt tcagatcaat cgtgatggtt tcaccatcat ttgcgccgac ctgaattttc
    16681 atcgagccat cttttgacag tacgttcacg ccgttgaact gggtttggcc ggatacgcgg
    16741 tcaatttcat caagacggga tttgatttcg tcctgaatgg agtccaggtc agaggtggag
    16801 ttagttccgg tcgtcgcctg aaccgtcagt tcacgaatac gctgtaagtt gttgttgatt
    16861 tcggacagcg cgccttcagt ggtctgtgca acagaaatac cgtcgttggc gttacgtgca
    16921 gcctgagtca ggcctttaat gttagaagta aaacggttag caatcgcctg acctgcggcg
    16981 tcatccttcg cgctgttaat acgcaagcca gaagacagac gctcgataga actcgacagc
    17041 gcagactggt tcttgttgat attattttga gtgatcagcg agaggctgtt ggtattaatg
    17101 acttgtgcca tgattcgtta tcctatattg caagtcgttg attacgtatt gggtttccac
    17161 ccgtcggctc aatcgccgtc aaccctgtta tcgtctgtcg taaaacaacc tttagaattt
    17221 ttttcacaaa cagccatttt ttgttagtcg ccgaaatact cttttctctg ccccttattc
    17281 ccgctattaa aaaaaacaat taaacgtaaa ctttgcgcaa ttcaggccga taaccccggt
    17341 attcgtttta cgtgtcgaaa gataaaagga aatcgcatgg caagtatttc atcgctggga
    17401 gtcgggtcag gtctggattt aagttcaatc cttgagagcc tcaccgccgc gcaaaaagcg
    17461 acgctaaccc ccatttcaaa tcagcaatcg tcgtttaccg ctaaacttag cgcctacggt
    17521 acgctgaaaa gcgcgctgac gactttccag accgccaata ctgcattgtc taaagccgat
    17581 cttttttccg ccaccagcac taccagcagc accaccgcat tcagtgccac cactgcgggt
    17641 aacgccatcg ccgggaaata caccatcagt gtcacccatc tggcgcaggc gcaaaccctg
    17701 accacgcgca ccaccagaga cgatacgaaa acggcgatcg ccaccagcga cagtaaactc
    17761 accattcaac aaggtggcga caaagatccg attaccattg atatcagcgc agctaactcg
    17821 tcgttaagcg ggatccgtga tgccatcaac aacgcaaaag caggcgtaag cgcaagcatc
    17881 attaacgtgg gtaacggtga atatcgtctg tcagtcacat caaatgacac cggccttgat
    17941 aatgcgatga cactctcggt cagcggtgat gatgcgctac aaagttttat gggctatgac
    18001 gccagtgcca gcagcaacgg tatggaggtc tcggttgccg cccagaatgc gcagctgacg
    18061 gtcaacaacg tcgccatcga gaacagcagc aacaccatca gcgacgcgct ggaaaacatc
    18121 accctgaacc tgaacgatgt caccacgggc aaccagacgc taaccatcac tcaggacacc
    18181 tccaaagcgc aaacggcgat taaagactgg gtgaatgcct acaactcgct aatagatacc
    18241 ttcagcagcc tgaccaaata caccgccgta gatgcgggag ctgatagcca gagttctagc
    18301 aatggcgcac tgctcggcga ctccacgctg cggacgattc agacgcagtt gaagtcgatg
    18361 ctgagtaata ccgtcagttc ttccaactat aaaacgctgg cgcagattgg tatcacgacc
    18421 gatcccagcg atggcaaact ggaactggat gccgacaaac tcaccgctgc actgaaaaaa
    18481 gatgccagcg gcgtaggtgc attgattgtt ggcgatggta aaaaaaccgg catcacgacc
    18541 accatcggca gcaacctgac cagttggctt tcgacaacgg gcattattaa agccgctacc
    18601 gatggcgtta gtaagaccct gaataaatta actaaagact acaacgccgc cagcgatcgc
    18661 attgatgcgc aggtcgcgcg ctataaagaa caatttaccc aactggacgt tttaatgacc
    18721 tcgttaaaca gcaccagcag ctacttaacg cagcagttcg aaaacaacag taattccaag
    18781 taatcaatat tcatcgggag acaggtcatg tacgcggcaa aaggcaccca ggcctatgca
    18841 caaattggcg tcgaaagcgc cgtaatgagc gccagccagc agcagctggt caccatgcta
    18901 tttgatggag tgctaagcgc actggttaga gcgcgcctgt ttatgcagga caacaatcag
    18961 caaggcaaag gcgtctcttt gtcaaaagcg atcaacatca ttgagaacgg actgcgggtg
    19021 agtcttgatg aagagagcaa agacgaatta acccaaaact tgattgctct ttatagctat
    19081 atggtcaggc gtttgctgca agccaattta cgcaacgatg tctccgcagt cgaagaagtg
    19141 gaagcattaa tgcgcaatat tgccgatgcc tggaaagagt cgttactctc cccttctttg
    19201 attcaggacc cagtctgatg aaccatgcac cgcatttgta tttcgcctgg caacaactcg
    19261 tcgaaaaaag ccagctcatg ttacgcctgg caacggaaga acaatgggac gaactcatcg
    19321 ccagcgaaat ggcgtatgtg aatgcggtgc aggagattgc acatctgact gaagagatta
    19381 acccatccac cacgatgcag gagcagctcc gcccgatgct gcgcctgatt ctcgacaacg
    19441 aaagcaaggt aaagcagtta ttacagattc ggatggatga actggcgaaa ctggtcggtc
    19501 agtcatcggt gcaaaaatcg gtgttaagtg cctatggcga tcagggcggc tttgtgctgg
    19561 ctccgcaaga taacttcttg tgaatcttaa caagctaatg gctcgcgaat aatctgatta
    19621 cggctacgct tctaatgttc cccttgaatg gagtcgaaga atgcgtaatc ccacgctgtt
    19681 acaatgtttt cactggtatt acccggaagg cggtaagctc tggcctgaac tggccgagcg
    19741 cgccgacggt tttaatgata ttggtatcaa tatggtctgg ttgccgcccg cctataaagg
    19801 cgcatcgggc gggtattcgg tcggctacga ctcctatgat ttatttgatt taggcgagtt
    19861 tgatcagaaa ggcagcatcc ctactaaata tggcgataaa gcacaactgc tggccgccat
    19921 tgacgccctg aaacgtaatg acattgcggt gctgttggac gtggtagtca accacaaaat
    19981 gggcgcggat gaaaaagaag ctattcgcgt gcagcgtgta aatgctgatg accgtacgca
    20041 aattgacgaa gaaatcattg agtgtgaagg ctggacgcgt tacaccttcc ccgcgcgtgc
    20101 cgggcaatac tcgaagttta tctgggattt caaatgtttt agcggtatcg accatatcga
    20161 aaaccctgat gaagatggca tatttaaaat tgttaacgac tacaccggcg aaggctggaa
    20221 cgatcaggtt gatgatgaat taggtaattt cgattatctg atgggcgaga atatcgattt
    20281 tcgcaatcat gccgtgacgg aagagattaa atactgggcg cgctgggtga tggaacaaac
    20341 gcaatgcgac ggttttcgtc ttgatgcggt caaacatatt ccggcgtggt tttataaaga
    20401 gtggatcgaa cacgtacagg aagttgcgcc aaagccgctg tttattgtgg cggagtactg
    20461 gtcgcatgaa gtcgataaac tgcaaacgta tattgatcag gtggaaggca aaaccatgct
    20521 gttcgatgcg ccgctgcaga tgaaattcca tgaagcatcg cgcatggggc gcgactacga
    20581 catgacgcag attttcacgg gtacattagt ggaagccgat cctttccacg ccgtgacgct
    20641 cgttgccaat cacgacaccc aaccgttgca agccctcgaa gcgccggtcg aaccgtggtt
    20701 taaaccgctg gcgtatgcct taattttgtt gcgggaaaat ggcgttcctt cggtattcta
    20761 tccggacctc tacggtgcgc attacgaaga tgtcggcggt gacgggcaaa cctatccgat
    20821 agatatgcca ataatcgaac agcttgatga gttaattctc gcccgtcagc gtttcgccca
    20881 cggtgtacag acattatttt tcgaccatcc gaactgcatt gcctttagcc gcagtggcac
    20941 tgacgaatat cccggctgcg tggtggtcat gtcgaacggg gatgatggcg aaaaaaccat
    21001 taatctggga gagaattacg gcaataaaac ctggcgtgat tttctgggga accggcaaga
    21061 gagcgtagta accgacgaaa acggtgaagc gaccttcttt tgcaacggcg gcagcgtcag
    21121 cgtgtgggtt atcgaagagg tgatttaaat tcatccccgg cggcaagccg gggagatttc
    21181 attacggcag tggcgtcggt aacggcgctt tatccagcgc ggcggcacac tctgccgtcg
    21241 ggcgatccac acgctccatc gtcataccat cgtactcaat ggtgttaccg tcacgctcaa
    21301 cctcgtatag ctcacgtttt acggtaacgt tagtgagatc atcagacatc aatgtcaact
    21361 tacccggcac ggcaatcacc cgctgccact gacggcaatc aagagtatcg ccctctttag
    21421 tgacaatcag gctgcctatc gcttccgggc tgaccagcgc acgttgcggc cctttggtct
    21481 gccagtagcc cgccagccag tccggcgcgg gggttttcac gacattgtta tagttttcga
    21541 cttcggcaca gcccgccagc aacatgagcg cgcctgcaat tgcgagtttt ttcatcattc
    21601 gtcctgcatg agaagaaaag aggattgtgg cattaaagcc ctgatgtcgc cagcctttcc
    21661 aggttcagag agaaatttga tctaactatt atttttgtga attattaccc cgaaaataaa
    21721 tttgcatgat gtatgattcg cgactcttgt ttcaaacctt ctgagttcag aggctacatt
    21781 catgtcatgg cagcaattca aacacgcctg gttgattaaa ttctgggcac ccatccctgc
    21841 ggtcatcgcg gcgggtattc tctccactta ctattttggc attactggca ccttttgggc
    21901 tgtcacgggt gaatttaccc gttggggcgg ccagctcctg cagctgtttg gcgtccatgc
    21961 tgaagagtgg ggttatttta aaattatcca tctggaagga tcgccattaa cccgcatcga
    22021 cgggatgatg atcctcggca tgtttggcgg ctgctttgcc gcagcgctgt gggccaacaa
    22081 tgtcaaactg cgcatgccac gcagccgtat ccgcattatg caggccatta ttggcggtat
    22141 tatcgctggt tttggcgcgc gtctggcaat gggctgtaac ctggcggcgt tctttaccgg
    22201 tattcctcag ttctcgctgc atgcctggtt ctttgccatc gccactgcca ttggttcatg
    22261 gtttggcgca cgctttaccc tgctgcccat cttccgtatt cccgtgaaaa tgcagaaagt
    22321 ttctgccgcc tcaccgctga cgcaaaaacc ggatcaggcg cggcgtcgtt ttcgtctcgg
    22381 gatgctggtc tttttcggta tgctgggctg ggcgctgctc acggcgatga accaaccaaa
    22441 actggggctg gcaatgctgt ttggcgtcgg ctttggttta ctgattgaac gtgcgcaaat
    22501 ctgctttact tcggcgttcc gcgatatgtg gatcaccgga cgtacccata tggcgaaagc
    22561 aatcattatc ggtatggcgg tgagtgccat cgggatcttc agttacgtac agttaggcgt
    22621 tgaacccaaa atcatgtggg cgggaccaaa cgcggtaatt ggtggtttac tgtttggttt
    22681 tggcatcgtg ctggcaggcg gctgcgaaac cggctggatg taccgcgcgg tagaaggcca
    22741 ggtgcactac tggtgggtcg gtctgggcaa tgtgatcggc tcgacgattc tggcgtatta
    22801 ctgggatgat ttcgctccgg cgctggccac cgactgggac aaaatcaacc tgctgaaaac
    22861 ctttggcccg atgggtggcc tgctggtgac atatttgctg ttgtttactg cgctgatgtt
    22921 gattatcggc tgggaaaaac gcttcttccg ccgtgcggca ccgcagactg ctaaggagat
    22981 cgcatgaaaa atatcgttcc tgattaccgc cttgatatgg tgggtgaacc ctgcccttat
    23041 ccggcagtcg caactcttga ggcgatgccg cagttaaaaa aaggggaaat cctggaagtg
    23101 gtgagcgact gtccacagtc gatcaataat attccacagg atgcgcgtaa tcacggctat
    23161 acggtgctgg atattcagca agacgggccg accattcgtt atttaattca gaagtaatcc
    23221 ctcattcccg ctgtggccca cagcgggaac atcgttgcct ctgacacata gattttaccc
    23281 gccccctctc ctattctttt cagtatgatc tcaggaggtt tctatgtgtg gacgctttgc
    23341 ccaatcccaa acgcgtgaag attaccttgc gcttctcgcg gaagatattg aacgcgatat
    23401 tccctacgat cccgaaccca ttggcagata caacgtcgcg ccgggaacca aagttcttct
    23461 gctcagtgaa cgtgatgaac accttcatct ggatccggtt ttctggggat atgctcccgg
    23521 atggtgggat aaaccgccgc tgattaacgc ccacgtagaa actgccgcaa ccagtcgcat
    23581 gtttaaaccg ctctggcaac atggtcgggc aatctgtttt gccgatggct ggtttgagtg
    23641 gaaaaaagaa ggcgacaaaa aacagccttt ttttatctat cgtgctgatg gacaacctat
    23701 ttttatggcc gcgataggta gcacaccatt tgagcgtggt gacgaagccg aaggattttt
    23761 aattgtcact gctgcagcag accaggggct ggtagacatt catgaccgcc gcccgctggt
    23821 tctgtcgcca gaagctgcac aggaatggat gcggcaagag attggcggta aagaagcatc
    23881 agaaattgct accaacggct gtgtgccagc aaaccagttc acctggcacc cggtatcgcg
    23941 cgcggtaggt aatgtcaaaa accagggcgc ggagttaatt caacctgttt gttgacatcc
    24001 tccacgccct gaaggacgag gatccctgct acgttcaggc tgtcgcctga atcatctcgg
    24061 tgggttcctg cttcaacggg cggcctgact gcaccatctc tccacaggca agaacggcgt
    24121 gccccgccgc taaaatgtta cgagcgccat ttacatcggc gttcgctgta tatccacata
    24181 cctggcatct gaatttactt tgtgacaggc ggttttcttt cgctgtatga ccacagcacg
    24241 cgcaacgctg gcttgtgtac gctggcggaa cagcaagcac ctgaccgcca cgccagagct
    24301 gcttgtactc aagctggcgg cgcatttcat accagccctg atccagtatc gaacggttta
    24361 aacctgattt tgcccggaca ttgcgcccgg ctggctgacc gtacccgctg ctgactttga
    24421 catgttgctg accttcaaat cctcaatgac tatcattgcg tggtttttgc tgacggtcgt
    24481 tgtgactttg tgaaggtagt ccctgcggat atttgcgata caggaatgca gtcgctgtat
    24541 tttgcgtttc tgctt
//
LOCUS       DAJOSY010000068        24179 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJOSY010000068 DAJOSY010000000
VERSION     DAJOSY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.068"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..892
                     /locus_tag="OHN27_002820"
     CDS             <1..892
                     /locus_tag="OHN27_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2287224.1"
                     /translation="NIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQI
                     GARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSA
                     PFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPESLEHA
                     VIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLT
                     PVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPK
                     HHEQSPAARNFRIHLLNALR"
     gene            1027..2247
                     /gene="mlc"
                     /locus_tag="OHN27_002821"
                     /gene_synonym="dgsA"
     CDS             1027..2247
                     /gene="mlc"
                     /locus_tag="OHN27_002821"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCQ2287225.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2372..3067
                     /gene="bioD"
                     /locus_tag="OHN27_002822"
     CDS             2372..3067
                     /gene="bioD"
                     /locus_tag="OHN27_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCQ2287226.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3020..4312)
                     /gene="clcB"
                     /locus_tag="OHN27_002823"
     CDS             complement(3020..4312)
                     /gene="clcB"
                     /locus_tag="OHN27_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCQ2287227.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLTSSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4471..5085)
                     /gene="dmsD"
                     /locus_tag="OHN27_002824"
     CDS             complement(4471..5085)
                     /gene="dmsD"
                     /locus_tag="OHN27_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCQ2287228.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5128..5982)
                     /locus_tag="OHN27_002825"
     CDS             complement(5128..5982)
                     /locus_tag="OHN27_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCQ2287229.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGVGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLFSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(5984..6601)
                     /gene="dmsB"
                     /locus_tag="OHN27_002826"
     CDS             complement(5984..6601)
                     /gene="dmsB"
                     /locus_tag="OHN27_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCQ2287230.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(6612..9035)
                     /locus_tag="OHN27_002827"
     CDS             complement(6612..9035)
                     /locus_tag="OHN27_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCQ2287231.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(9096..11522)
                     /locus_tag="OHN27_002828"
     CDS             complement(9096..11522)
                     /locus_tag="OHN27_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCQ2287232.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRNAAASV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPEDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(11721..12026)
                     /locus_tag="OHN27_002829"
     CDS             complement(11721..12026)
                     /locus_tag="OHN27_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCQ2287233.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSSQDGAPAEPQ"
     gene            12134..12844
                     /locus_tag="OHN27_002830"
     CDS             12134..12844
                     /locus_tag="OHN27_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCQ2287234.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(12847..13407)
                     /gene="speG"
                     /locus_tag="OHN27_002831"
     CDS             complement(12847..13407)
                     /gene="speG"
                     /locus_tag="OHN27_002831"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCQ2287235.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(13442..13783)
                     /locus_tag="OHN27_002832"
     CDS             complement(13442..13783)
                     /locus_tag="OHN27_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCQ2287236.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNNTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            13918..14244
                     /locus_tag="OHN27_002833"
     CDS             13918..14244
                     /locus_tag="OHN27_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCQ2287237.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            14450..15664
                     /gene="rspA"
                     /locus_tag="OHN27_002834"
     CDS             14450..15664
                     /gene="rspA"
                     /locus_tag="OHN27_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCQ2287238.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEAPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            15676..16695
                     /locus_tag="OHN27_002835"
     CDS             15676..16695
                     /locus_tag="OHN27_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HCQ2287239.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFTEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPIVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            16753..16863
                     /locus_tag="OHN27_002836"
     CDS             16753..16863
                     /locus_tag="OHN27_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCQ2287240.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHTC"
     gene            complement(16883..18163)
                     /locus_tag="OHN27_002837"
     CDS             complement(16883..18163)
                     /locus_tag="OHN27_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCQ2287241.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(18198..18449)
                     /locus_tag="OHN27_002838"
     CDS             complement(18198..18449)
                     /locus_tag="OHN27_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCQ2287242.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(18522..20993)
                     /locus_tag="OHN27_002839"
     CDS             complement(18522..20993)
                     /locus_tag="OHN27_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCQ2287243.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKACTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            complement(21087..21278)
                     /gene="ydfD"
                     /locus_tag="OHN27_002840"
     CDS             complement(21087..21278)
                     /gene="ydfD"
                     /locus_tag="OHN27_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HCQ2287244.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(21275..21463)
                     /gene="dicB"
                     /locus_tag="OHN27_002841"
                     /gene_synonym="ftsT"
     CDS             complement(21275..21463)
                     /gene="dicB"
                     /locus_tag="OHN27_002841"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCQ2287245.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            21544..22065
                     /locus_tag="OHN27_002842"
                     /pseudo
     CDS             21544..22065
                     /locus_tag="OHN27_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            22079..22831
                     /locus_tag="OHN27_002843"
     CDS             22079..22831
                     /locus_tag="OHN27_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCQ2287246.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEASTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(23109..23198)
                     /locus_tag="OHN27_002844"
     CDS             complement(23109..23198)
                     /locus_tag="OHN27_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287247.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(23253..23465)
                     /gene="cspF"
                     /locus_tag="OHN27_002845"
     CDS             complement(23253..23465)
                     /gene="cspF"
                     /locus_tag="OHN27_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HCQ2287248.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            23766..23981
                     /gene="cspB"
                     /locus_tag="OHN27_002846"
     CDS             23766..23981
                     /gene="cspB"
                     /locus_tag="OHN27_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HCQ2287249.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT         6013 a         5735 c         5994 g         6437 t
ORIGIN      
        1 gaatattgaa cttcgtcatc tgcgttactt tgttgctgtt gcggaagagc tgcatttcgg
       61 gcgcgccgct gcccgcctga atatttcgca accgccgcta agtcagcaga ttcaggcgct
      121 ggagcaacaa attggtgccc gactgctggc acgaaccaat cgcagtgtat tgctgacggc
      181 agcaggaaaa cagtttcttg cagatagtcg gcaaatcctg tctatggtgg atgacgctgc
      241 cgctcgcgct gaaaggctgc atcagggtga agcgggggag ttgcgcattg gttttacttc
      301 gtcggctcct tttattcggg cggtgtccga tacgttatcg ctgtttcgcc gtgattatcc
      361 tgatgtccat ttacaaaccc gcgaaatgaa cactcgcgag caaatcgctc cgctcattga
      421 aggaacgctg gatatgggat tgctgcgtaa cacagcgtta ccggagtcgc ttgaacacgc
      481 agtcatcgtc catgaaccgc ttatggcgat gatcccgcac gatcatcccc tggcaaataa
      541 cccgaatgta acgctggctg aactggcgaa agaacccttt gtcttttttg atccgcacgt
      601 cgggacaggg ctgtatgacg atattctcgg gctgatgcga cgttaccatt tgacgcccgt
      661 catcactcag gaggtgggcg aggcaatgac catcatcggt ctggtttccg ccggtctggg
      721 tgtttcaatt ttgcctgcgt catttaaacg tgttcagctc aacgaaatgc gctgggtgcc
      781 gattgctgaa gaggatgcgg tttctgaaat gtggttggtc tggccgaaac atcatgaaca
      841 aagtccggct gcgcgtaact ttcgtattca tctgctgaat gctctcaggt gagggaaatt
      901 tcagcgaaaa agcccgaaaa atgtgctgtt aatcacatgc ctaagtaaaa atttgacgac
      961 acgtattgaa gtgcttcacc atagcctaca gattatttcg gagcgcgaaa atatagggag
     1021 tatgcggtgg ttgctgaaaa ccagcctggg cacattgatc aaataaagca gaccaacgcg
     1081 ggcgcggttt atcgcctgat tgatcagctt ggtccagtct cgcgtatcga tctttcccgt
     1141 ctggcgcaac tggctcctgc cagtatcact aaaattgtcc gtgagatgct cgaagcacac
     1201 ctggtgcaag agctggaaat caaagaagcg gggaaccgtg gccgtccggc ggtggggctg
     1261 gtggttgaaa ctgaagcctg gcactatctt tctctgcgca ttagtcgcgg ggagattttc
     1321 cttgctctgc gcgatctgag cagcaaactg gtggtggaag agtcgcagga actggcgtta
     1381 aaagatgact tgccattgct ggatcgtatt atttcccata tcgatcagtt ttttatccgc
     1441 caccagaaaa aacttgagcg tctaacttcg attgccataa ccttgccggg aattattgat
     1501 acggaaaatg gtattgtaca tcgcatgccg ttctacgagg atgtaaaaga gatgccgctc
     1561 ggcgaggcgc tggagcagca taccggcgtt ccggtttata ttcagcatga tatcagcgca
     1621 tggacgatgg cagaggcctt gtttggtgcc tcacgcgggg cgcgcgatgt gattcaggtg
     1681 gttatcgatc acaacgtggg ggcgggcgtc attaccgatg gtcatctgct acacgcaggc
     1741 agcagtagtc tcgtggaaat aggccacaca caggtcgacc cgtatgggaa acgctgttat
     1801 tgcgggaatc acggctgcct cgaaaccatc gccagcgtgg acagtattct tgagctggca
     1861 cagctgcgtc ttaatcaatc catgagctcg atgttacatg gacaaccgtt aaccgtggac
     1921 tcattgtgtc aggcggcatt gcgcggcgat ctactggcaa aagacatcat taccggggtg
     1981 ggcgcgcatg tcgggcgcat tcttgccatc atggtgaatt tatttaaccc acaaaaaata
     2041 ctgattggct caccgttaag taaagcggca gatatcctct tcccggtcat ctcagacagc
     2101 atccgtcagc aggcccttcc tgcgtatagt cagcacatca gcgttgagag tactcagttt
     2161 tctaaccagg gcacgatggc aggcgctgca ctggtaaaag acgcgatgta taacggttct
     2221 ttgttgattc gtctgttgca gggttaacat tttttaactg ttctaccaaa atttgcgcta
     2281 tctcaatttg ggccaggaaa gcataactta gactttcaag gttaattatt ttcctggttt
     2341 atatttgtga agcataacgg tggagttagt gatgctgaag cgtttcttta ttaccggtac
     2401 agacacttct gtagggaaaa cggtggtttc ccgcgcattg ctacaagcgt tagcctccca
     2461 gggaaaaacg gttgcgggat ataaacccgt agcgaagggg agcaaagaga cacccgaagg
     2521 gctgcgtaat aaagatgccc tggtgttgca gagtgtttca accatcgaac tgccttatga
     2581 agcagttaat cctatcgcgt taagcgaaga agaaagtagc gtggcgcaca gttgcccaat
     2641 caattacacc ctcatttcaa acggcctggc aaacctgacc gaaaaagtcg atcatgtcgt
     2701 ggtagaaggg actggcggct ggcgcagtct gatgaatgat ttgcgtccac tctctgaatg
     2761 ggtagtgcag gaacaactgc cggtgttgat ggttgtcggt attcaggaag gttgcattaa
     2821 ccatgcactg ctaacagctc aggcgatcgc caacgacggg ctgccgctca ttggctgggt
     2881 ggctaaccga atcaacccag gactggcgca ttatgcggaa atcattgatg tgctgggtaa
     2941 aaaacttccg gcaccgctca ttggtgaact gccttatctg ccgcgcgccg aacagcgtga
     3001 actggggcaa tacatccgct tagctatgct gcgcagtgtg ctggcggtag atagagtcac
     3061 ggtgtaacgt ccgcgaaatt accgacgcaa ttacgcaggc aatcaataaa ccggggagta
     3121 gctgatactc cccggtcatt tcacatatca tcaacgtcga cataatcggc gcgtgcgtgg
     3181 tcgccgccaa cagtgtcgcc atcccggtca atccgagtaa aagtgtaatt tcttcgccat
     3241 cagggaacca taatcccagg ctacgaccat acaacatgcc aatggcaaga ccgataaata
     3301 gcgtcggtgt aaagacccca ccgggtgcgc cggaaccgct actcgtcagc acggcacaca
     3361 gtttacaaag gaagatcccg gcaatgatca ttaacagtgg tggggcggtt aaaaaggatt
     3421 gtacggtgct atagccgttg ccccacactg caggtgtgaa cagggaaagc agacccacga
     3481 tcaacccgcc tagtgccagt tgccagggcg gcgcaagttt gagactcaca aatccacgat
     3541 gacaggcgtt cattaacgtt aacaacagtg gtccgcacag acctgccagc acacctgtac
     3601 tgataatcag cgcatagtca cgagcctgaa ccgtcactga gagttgtacg ttgtagagta
     3661 acgcgtcgct atgattaatc agattgctaa ccagcaatgc cacgacggcg gaaataatca
     3721 ccgggccgag agaggccaac atcatagtgc caaacagcac ttcggctata aataaactgc
     3781 cagcaagcgg ggcacgatag gccgcagcca ttcccgccgc ggccccacag gcgatccata
     3841 atttccactc ctggcgtggc gtaaaacgtt gggcaaaaca ggaggcggca agggcagcta
     3901 aaagaatcat cgcaccttcg cgaccaattg cactgccgct ggttactacc agcagagagg
     3961 caagcgattt aaccaggctt gctgcgtaat cgaactgtcc atcggtttgc aacgcttcca
     4021 tgtaatcggt cggcgcatga gggcgttgtt gggtaaattt ctgccagccc atcagcaaca
     4081 aacccgccgc cagtccgccg agcgccggag ttagcaaccg tcgccagggg gaaaggtttg
     4141 tcgctgcatt gaccagactg ccggagtcat tattgaggaa caaccactcc agtagcagca
     4201 tcgcatgacg aaacccggca acggcaaagg ccgcgagaat accgacgact gttgcgataa
     4261 gcagacggcg gaacatagtg cgcagatccg gatagatgtg tagatgatgc atgagcagaa
     4321 atgggcaatg aaacacgggc gactattttg cggcaaaaga ggccagccgc aaaagcagtg
     4381 cgtgctttaa gagataaaaa aacgtgacac tgtaacctat tattgccgga ttgcgcgtaa
     4441 tcgtcaccat ccggcaatat tacggtgatc ctatcgaaac agcggtttaa ccgcgacagg
     4501 aattaacagt tgtgattgcc attgcgccag cgttaagcga gccagttcac ccagtgcacg
     4561 gtagaaaggg tgttctgctt tttcgataaa aacatcaaga aaacgtgttg accacggaaa
     4621 aaggtgccat gccagcaatt cttcgcattc tgtctggcga ccattctccg ccaaccacgc
     4681 agccatcaac agcagtgaac caaaatgatc ttccggttcg ttttgcttca tttcaaactg
     4741 aatgcctttc tcgcgcatcc actgacgaag tgccaatgtt gaatcgccaa acagcacaga
     4801 ttcgcgatcc agccagaccg acccccacgg cggagacggc agtgcccacg ggccgacaaa
     4861 caaacgctgc caggcctggg cgtgagtctc ttcacactgt gtctgaaaag ccgtcaccag
     4921 cggcgctaat gacgcttctg gtagaggcca ctgagtttcc cagccgtcac tggtgagtac
     4981 cgcaaccaga ggtgcggctt ccgcactctc tggagcgtaa taaaacagcg cgcccaacac
     5041 gcgcgccgcg acagaaaaat tatcttgctg tgaaaaatgg gtcattccta acatcctgaa
     5101 atgtgcgggt tgccccgcac caatttgtta acctgcaata gccattccta ctgtcatgtg
     5161 caggccataa aagagcacac gcccgatcat ctcaccgcca agtatgagga ttagccccag
     5221 aataagtccg gcaacgtgag gttcacggcg acgaatgagc gggcaaagcc agcatccaag
     5281 acctgcgcat aacaatacca cacgccagac ctgtaaggag gcgtaatctg gtaccagtgc
     5341 gctggcttgt tgcacggatg agtgaataga cgccagagac aaaccttgca gcacaatcac
     5401 cccggcacaa gcgatcagcg ccagaacgct aatgatggca aatggtgtgg tattaaatgt
     5461 aacgcgagct gcccgcagaa ttgcggcagc aagtattgga ccgctcaaca ataccgtcag
     5521 gaagaaagcc agcgtagtgt aaccgttatg ccaggttggc acggtgtcga tttgatacac
     5581 gcaggtcatc atccagacga aaatgacgcc gagcgccatg ctgaaaagta accagagttt
     5641 ccccaacgct tgcggcattt taccgatgac tgccaccagc caccataatc cgccaacggc
     5701 aaaaaatatg gaaccggcag caatctcgtt actcagacca gaagctccga ttcgattcag
     5761 cgagttaaac gcccgcagtg gcgatcccag gtgcatgaca gaggcaataa acccgacgcc
     5821 catcaacagc cagaggaaaa acatgccacg gacaatacgc tgacggtctg cgtcattttt
     5881 ggcggcgaac cagccaattc cgctaacaat cagcgcgcct actacgcatt gacccaatac
     5941 ggtaaagata accagtggcc actcatgcca tccatttccc attttacact tcctccggat
     6001 tagccagata accagtggta tcgccggtcg ggcggctgtt ggcgttaggt ttgataacga
     6061 tattgggttt tgtgaaatgc gcgcgcggca gtggcgcgac ggcagcaaga gtgccgtgct
     6121 tctggcgcag ctcttcaatt ggaccgaact caagtgcacg cagcgggcag gattcgacac
     6181 atatgggttg tttcccctcg gcgacgcgcg aataacaacc atcgcacttc gtcatgtgcc
     6241 ctttttcagc attgtactgt ggcgcgccgt acggacaagc catgtggcag tagcggcagc
     6301 cgatacaaac atcttcatca accaccacaa agccatcttc acgcttatgc attgcgccgc
     6361 tggggcagac ttttgtacat gcggggtcat cgcaatggtt acaggagatg gagagataat
     6421 aagcgaacac gttctggtgc cagacgccat tatcttcctg ccagtcgccg ccagcgtatt
     6481 cataaatacg gcggaaactg acttccgggc caagatcttt aaaatctttg cacgccagtt
     6541 cgcaggtttt acagccagta cagcggctgg aatcgataaa aaatccatat tgtgtggtca
     6601 tgggctactc cttaaacctt ttcgatctgg acaagattgc tgtgcgacgg gtttcccttt
     6661 gccagcggtg aagggcggtg agaggtcaga atattgatac tgccgccgtg atcgacccgg
     6721 tcaccaaaca tatccgcttt aagccacgca ccttgcccga tggcggtaac gccaggcaga
     6781 atacgcggag tcacttttgc ggcaatcagc atttctccat tattgttaaa tacccgcacg
     6841 gtatcgccat gacggatacc gcgtgcctga gcatcaatgg ggttgatcca cacctcttgt
     6901 gggcaggcct gctgtaacac atcaatattg ccgtagctgg agtgggtacg cgctttgtaa
     6961 tggaagcccg ttaactgcag tggataggtt ttccgcaggg gatcgtccca gccatcaaaa
     7021 cctggggtat acgcaggaag gggatgaata atttcatctt ttttcaattc ccaggtatct
     7081 gcaatcttcg ccagtcgttc agaataaatt tcgattttcc ccgaaggtgt tttcaacggg
     7141 tttgcctgtg gatcttcacg gaatgcgcgg aaagcgacgt agtgttcttc cgggcatttt
     7201 ttcttaaaga tcccggtcgt tttcatctcc tcgtagtcgg gcatctcagg gttacgttcc
     7261 ttcgttttcg catggagata tttgatccat tcatgctgac tgcgaccttc agtaaaggtt
     7321 tgataaacgt ctggtcctaa gcgtttggcg acttcactca gcatccagta gatgggtttg
     7381 cgttcaaatt ttgctgaggt tgcgggttgg gcgaggatca catagcccat attccctgca
     7441 gattcatgag agataaggtc ttcttgctct gttggcatca ggtcgggcaa caggatatcg
     7501 caatacttag ccgaggccgt catgaagtgg tcaatgccaa caatcatctc gcacttgctg
     7561 tcatcctgaa gcacctcatg ggtgtgattg atgtcgccat gttgattgat caatgtgtta
     7621 ctggcgtagc accataaaaa cttgatgggg acatccagtt tttcttttcc acgaacacca
     7681 tcacgggtcg cggtcatttc cgtaccatgg tcgatggcat ctgtccatgt aaagacggaa
     7741 atctgcgttt taacaggatt ctcgagcatc gggaaccatt ctacccccag atcccagcta
     7801 ccttcgcgta cgcctgagtt gccgccgttt atgccgacgt tgccggtgag aacggaaagc
     7861 atggcaatag cgcgggatgt ttgctcgccg ttggaatgtc gttgtggccc ccaaccctga
     7921 caaatataag caggttttgc tgaaccgatc tctcgtgcca actggataat tttttctgcc
     7981 gggatgctgg tgatttttgc tgcccattcc ggcgttttag ctatgccgtc aggcccttcg
     8041 cccagaatat aggctttata atgcgcgtta cgtggtgcgt tggcgggcag cgttttttca
     8101 tcgtaaccaa cacaatattt gtcgagaaat ggctgatcga ccatgttttc agtaatcagt
     8161 acccaggcaa tcgcacaggc cagtgcgcca tcggtgccag ggcgaatggg cagccattca
     8221 tcttcacgcc cggcagcagt gtcgttataa cgtggatcga tgacgatcat gcgtgcgttt
     8281 gaacgttcgc gggcttgctc gacgtagtaa gtgacaccac cgccgctcat ccgcgtttct
     8341 gccgggttat ttccgaacat aacgaccagt ttcgtattgg cgatatcatc cgggctgttg
     8401 ccatcattgg caccgaacat ataactcatt gcggcactga tctgtgcggt actgtagctg
     8461 ccatagcgac tgagaaaacc accgcaagag ttcatcagac ggtacgggac gtttgagttg
     8521 gtgatgtttc cgccatctac gcctgttccg tacaggacat gtacagcctc attgccgtaa
     8581 tctttcagga tccgccgaag attatcactg atggtatcca gggcttcgtc ccaacttatc
     8641 cgttcaaatt taccttcacc gcgcttgccg acgcgcttca tgggatattt caacctatca
     8701 ggatgattca tccgtcggcg gatagagcgc ccgcgtaaac acgctcgaac ctgatgatta
     8761 ccgtagacgt cgtcacctgt cgtatcagac tccacccagt acacggtgtc atctttcaca
     8821 tgcaaacgta acagacagcg gctcccgcag ttaacggtgc aggaactcca gaccgctttc
     8881 tcttctaccg gagcctctgc cgcccggacc atttgggaaa atggcagagt gaaagcactg
     8941 cttgccagcg caagactgcc aagtgcggag gttttcatca gacttctacg gctgatttca
     9001 gccttcatga gcgcctctgt ggtatggatt ttcatcatta ctcacttatt gcttttcaaa
     9061 caaaatgtca tgccagaatt tatggttgtc gtgggttata ttttttcgat ctcgaccaga
     9121 ttagtgtgct gcgggtttcc cttcgccagt ggtgaagggc gcagagtggt tagcgtattc
     9181 acacagccgc catggtcgat tttatcgcca gacatattgg cctcgtgcca ggctccctgg
     9241 cccatagcgc taactccagg gagaatacgt ggtgttactt tggctggtag ccgaacttcg
     9301 ccacgatggt taaacacccg caccatatcg ccgttggcaa tcccacgttt ctgcgcatct
     9361 atagggttga tccacacctc ctgacggcag gcagccttca ggagatcaat attgccgtag
     9421 gtcgagtgag tacgggattt gtaatggaaa ccaaacagtt gcagtgggaa ggttctacgt
     9481 tcaggggagt tccagccttc aaaggttgag gcataaactg gcaatgggct tatcacttca
     9541 tctttttcca gttcccaggt acgggcaatt tccgccagcc tgctggaata aatttcaatc
     9601 ttaccggaag gcgttttaag tggatttgcc tcggggtcgt cacgaaatgc tttgtaggcg
     9661 acaaaatggc cattgggatc tttacgctta tagataccca tttttttcag ttcgtcgtaa
     9721 gacggtaacg ccggatcttt ggcaagcatt ttggcgtaca gatgttgtaa ccattgttcc
     9781 tgcgtgcgac cttctgtgaa cttttgatag acgtcaggtc caagacgttt cgcgacttca
     9841 ctcaggatcc agtaaatcgg tttgcgttcg aatttttcgc tggtgacagg ctggaggaaa
     9901 atgagatatc ccatgttacc ggcgtagtcg ttaggaataa tatcttcctg ctcaacggtc
     9961 atcaggtctg gcagcagaat gtcggcatat tttgccgatg aggtcataaa gttttcgatg
    10021 accacaatca tttcgcattt cgattcgtcc tgcagaattt catgcgtttt gttgatgtca
    10081 gaatgctgat taacgagggt atttcccgcg tagttccaga tgaacttaat gggcacatcc
    10141 agtttatctt tgccgcggac gccgtcgcgg attgccgtca tttgcggacc atgatcgata
    10201 gcatctgtcc agctgaagca ggagattgac gttttgaccg gattatccag caccggcagg
    10261 cgttctatgg taatggtata ggtcgattca cgcgcgccac tatttccgcc gctgatgccg
    10321 acattgcccg tcaaaatagg taacatagca atagcgcgtg cagtcagttc gccgtttgcc
    10381 tggcgttgtg gcccccagcc ctggcagata taagcgggtt ttgctgtgcc aatttcacgc
    10441 gccagtttga tgatacggtc ctccgggata ccggtaattt gcgaagccca ctgcggcgtt
    10501 ttcgctgttt tatcgtcacc ttcaccaaga atataggctt tatagtgacc atttttgggt
    10561 gcatctgcgg gtaaggtttt ttcgtcatag ccgacgcagt atttatcgag aaaaggttga
    10621 tcaacgagat tttcgttaat caatacccag gcaatacccg caaccagcgc ggcatcggtg
    10681 cccgggcgaa tagggagcca ttcgtcttca cgaccggcag ccgtatcggt atatcgcgga
    10741 tcgataacaa tcattttggc gttcgatttc tcgcgcgctt tttcaagaag ataagtgatg
    10801 ccaccaccgc tcatgcgggt ttctgccggg ttgttaccaa acatcacgac cagcttgctg
    10861 ttttcaatat ccgtggtgct gttgccatca ttactgccgt aggtgtaggg catggcacag
    10921 gaaatttgcg cagtgctgta ggagccatac tggttgagtg aaccgccgta gcagttcatc
    10981 aggcgtttga ccgccgaggc tgatggcgaa gagcgggtca tattgccgcc aacgatcccc
    11041 gaagagtact gaatatatac agcctcattg ccatattgtt cgacggtttt cttcaggcta
    11101 ctggcgatag tatccagggc ttcatcccag ctaatccgtt cgaatttgcc ttcgccgcgt
    11161 ttgcccacgc gtttcattgg gtaattcaag cgatcgggat gattaatacg ccggcggatg
    11221 gagcgaccgc gcaaacaggc gcgtacctga tggttgccgt actcatcgct gccggtattg
    11281 tcagtttcca cccaggtcac ttcattatct ttaacatgta gacgaagtgc acagcggcta
    11341 ccacagttga cggaacaggc accccagacc actttttcgc gggcctgttg taccgatgct
    11401 gctgcattgc gcagggtaaa cggcaaagaa aaaccgcctg cagccagcgc cagagaacct
    11461 atcgcggtag atttaacgag tgttctgcgg ctgatgccca ccattcgttc atttttggac
    11521 ataactcact ccctgttctt tatcgttata taaaagttta tatattgaat atttagcgcg
    11581 ctaacaatag agggagtcta cccattttgg gttaagaatt attaatccat atcaatagaa
    11641 gggtatgagt aataaggtgg gattatgttg tatgttcaaa tcgccggatt tgtcatatcc
    11701 ggcgttcagt cgataatgtg ttactgcggt tcggcaggcg cgccatcctg gctagactgc
    11761 gcgggagcag agacgttacc gctggtggtg cgggtataga gaattttatg cgtatcatta
    11821 gcgcaatggc cgacgacctg ggaatcaggc tgatcaacct ggtcattggg tacaatactt
    11881 aacgtgaagc tgctttcggg tacgccatta ttgataatgc gctgtgatat atcgctctgt
    11941 atgcgctcac aggatcccgg cgcggcgagt accgcgggtg aggcgagggc gagcagaagc
    12001 gcggcacagc aggttgagag tttcatcata agctccttac gcgaagataa cttctttaag
    12061 catagcattt aacgtgtaaa gtactgtatt tgctactatg attgagaatc atctctactc
    12121 tctggtgact gttgtgaaat acaaattact accatgctta ctcgcgatat tcctcacagg
    12181 atgtgaccgc acagaggtaa cactttcatt tacccctgag atggccagtt tctctaatga
    12241 attcgatttt gatccgctgc gtggtccggt aaaagatttc actcagacat taatggatga
    12301 gcaaggtgaa gtgacgaaac gtgtttctgg gactttgtcg gaagaaggct gttttgattc
    12361 actcgaatta ctggatctgg aaaataatac cgtggtcgct ctggtactgg acgccaatta
    12421 ttaccgtgat gccgagacgc tggagaagag agtacgttta cagggaaaat gccagctagc
    12481 agaattacct tctgccgggg tgagttggga aaccgatgat aatggcttcg tgattaaagc
    12541 cagcagcaaa caaatgcaga tggaatatcg ctatgatgat cagggttatc cgctgggtaa
    12601 aaccacgaaa agtaacgaca aaacattatc tgtcagcgcc acgccatcaa cggatccgat
    12661 caaaaaatta gattacacag cggttacttt actgaataat caacgggttg gtaatgtaaa
    12721 acagagctgt gaatatgaca gtcacgctaa tccggtggac tgtcagctaa tcattgttga
    12781 tgaaggagta aaacccgccg tcgaacgggt ttacaccatc aaaaatacga tcgattatta
    12841 ttaatgctat tgtgcggtcg gcttcaggag agtctgaccc ggtgttttgt gctctgccag
    12901 atactgatgc tggaatatac acatgcgaat ggcattacga tattgaccat taataaagaa
    12961 ctcgtgcatc aattcacctt caaccgaaaa gccaagcttg cggtaaatgt gaatcgcttt
    13021 ttcattctct ttatcaacga tcagatacag cttatagaga ttgagaacgg taaagccata
    13081 gtccattgct aatttggcgg cacgggttgc cagacctttc ccctgatact ccggggagat
    13141 aattatctga aattctgcgc ggcgatgaac atggttaatt tccaccagct ccaccagacc
    13201 ggctttttcg ccgtcacatt ccaccacaaa gcgccgttcg ctctgatcgt gaatatgctt
    13261 atcatacaga tcagagagtt caacaaaggc ttcgtagggt tcctcaaacc agtaacgcat
    13321 cacactggcg ttattgtcga gttgatgtac atagcgtaaa tcttcacgct ccagcgggcg
    13381 tagcttaaca ctgtgggcgc ttggcataac gtgtccttac attccttaaa tcaataacag
    13441 gttagggggt aataacgcgg ccagttcgac ggtccaggca gcgcaaagta ttgggctccc
    13501 agtaggcatt gatgttggcg ctttgctcac atttatcgcg gttatcaaaa gcggcgtcgg
    13561 ctttatccca ctctttttca gtgcgtttat tcactttctg gcgcagattg cgcgtgttat
    13621 tccattgctc tttttccata gcggcgtgct ggcggctttg tgcactgtcg ccagactcaa
    13681 tcaccagttt gttagtttcg gcatgaacag ttgtgctcaa tgccagtgcg caaggcagca
    13741 gaatagcgag caggccgatt cgtttgctga gagtgatttt cataattcat tccctgtatg
    13801 aatgattaaa ggtgattcta caccatccac tgcggacgca aaacgtacca ggagggtgtt
    13861 tatattgatg atattatgtc gccctataac tatacatgat gtcaataaga gacaaagatg
    13921 attaaaacaa cgttactatt ttttgctact gcgctgtgtg aaattattgg atgctttctg
    13981 ccctggttgt ggttaaaacg aaacgccagt atctggctgt tgcttccggc ggggatttca
    14041 ctggcgctgt ttgtctggtt gttaacgttg catccagcgg cgagtgggcg tgtttacgcg
    14101 gcttatggtg gcgtttatgt ctgcacggcg ttgatgtggc tgcgcgttgt ggatggcgtg
    14161 aaactgactc tttatgactg gacgggtgcg ttgattgcgc tttgcggcat gttgatcatt
    14221 gttgcgggct gggggcgcac gtaggaacat aaatccattt tatcaataag ataagaggaa
    14281 gtgtcagctg acaaaaggta ttctatttca tcttttgtca accattcaca gcgcaaatat
    14341 acgccttttt ttgtgatcac tccggctttt ttcgatcttt atacttgtat ggtagtagct
    14401 caattgcgta gatttcatgc atcacgacaa gcgatgcaag gaatcgaaca tgaagatcgt
    14461 aaaggctgaa gtttttgtta cctgtccggg gcgtaatttc gtcacattaa aaatcaccac
    14521 tgaggacggt attacgggcc ttggggatgc caccctcaat ggacgtgagc tttccgtggc
    14581 ctcttatttg caggatcacc tttgtccgca gcttattggt cgcgatgcgc accgtatcga
    14641 agatatctgg cagtttttct ataaaggtgc ttactggcgt cgcggtccgg ttacgatgtc
    14701 ggccatttca gcggttgata tggcgctgtg ggatattaaa gccaaagctg ccaacatgcc
    14761 gctttaccag ttactcggcg gcgcgtctcg tgaaggggtg atggtttatt gccataccac
    14821 cggtcacagt attgatgaag ctctggatga ttatgcccgt catcaagagc ttggattcaa
    14881 agccatccgc gtgcagtgcg gaatccctgg tatgaaaacc acctacggca tgtcgaaagg
    14941 taaaggtctg gcttatgaac ccgcaaccaa aggacagtgg ccggaagagc agctgtggtc
    15001 gacggagaaa tacctcgatt tcatgccgaa attgtttgac gcggtacgta acaagtttgg
    15061 ttttaatgaa catttgctgc atgacatgca ccatcgctta acgcctattg aagcggcgcg
    15121 ctttggtaaa agcattgaag attatcgcat gttctggatg gaagccccga cgcctgcgga
    15181 aaaccaggaa tgcttccgtc tcattcgcca acataccgtc acacccatcg cagtgggtga
    15241 agtcttcaac agcatctggg actgcaaaca actgattgaa gagcaactca tcgattatat
    15301 ccgcaccacg ctgacccatg caggcggaat taccggtatg cgccggattg ccgattttgc
    15361 ttcgctgtat caggtacgta ctggctcaca cggtccttcc gatttgtcac cagtctgcat
    15421 ggctgcggcg ctgcactttg atctgtgggt ccccaatttc ggtgtccagg aatacatggg
    15481 ttattccgaa caaatgctcg aagtcttccc gcacaactgg actttcgata acggctatat
    15541 gcatccggga gacaaaccgg gtcttggtat cgaattcgat gaaaagctgg cggcgaaata
    15601 tccctatgaa cctgcttatc taccagtcgc acgtctggaa gatggcacgc tgtggaactg
    15661 gtaaggagta agataatgaa aagcatatta attgaaaaac cgaatcaact ggcgattgtc
    15721 gaacgtgaaa tacccacccc gtcagcgggt gaagtacgag taaaagtgaa acttgccgga
    15781 atttgtggtt cagatagcca tatttatcgt gggcataatc cttttgcgaa atatccgcgc
    15841 gtcattggtc atgaattctt tggcgtcatt gatgcagtgg gtgaaggcgt ggaaagcgcc
    15901 agagtcggtg aacgtgttgc tgtcgatccg gtggtcagct gtgggcattg ctatccgtgc
    15961 tctataggta aaccgaacgt ttgtacgaca ctggctgtat taggtgtgca cgctgacggt
    16021 ggtttcagtg aatatgccgt ggttccggca aaaaatgcgt ggaaaattcc tgaagcagtg
    16081 gccgatcaat atgcggtaat gatcgaacct tttaccattg cggctaacgt aaccggacat
    16141 ggtcaaccga ctgaaaatga taccgttctg gtttatggtg ccggtccaat cggcctgacg
    16201 atcgttcagg tattaaaagg cgtctataac gttaaaaatg tgattgttgc cgatcgcatt
    16261 gatgaacgac tggaaaaagc gaaagagagc ggggctgact gggcgattaa taacagccag
    16321 acaccgcttg gcgagatttt cactgaaaaa ggcatcaagc cgacattaat tatcgatgcg
    16381 gcttgtcatc cttctatcct gaaagaggcc gtaacgctgg cttctccagc ggcacgtatt
    16441 gtattgatgg ggttctccag tgaaccgtct gaagtgattc agcaaggaat taccggaaaa
    16501 gaactctcta ttttctcttc acgcttaaat gcaaataaat tcccgatcgt tatcgactgg
    16561 ttaagtaaag ggttaattaa accagaaaaa ttaattaccc atacgtttga tttccagcat
    16621 gttgctgatg ccattagttt atttgaacag gatcaaaaac attgctgcaa agtcttactc
    16681 actttttctg aataatacca ataacggcga gtaagtagta cgcatcttac ctctttttta
    16741 gagataacca ttatgacaat agaaaaacac gaaagaagca ctaaggattt ggtgaaagca
    16801 gcagtatcgg gatggctggg cactgcgctt gaatttatgg atttcaagag tcatacgtgt
    16861 taactatttg ataaatatta aattaatttt tcattgcttc gttatggggc atggttgggg
    16921 caaactcgct taactgtgta tttaacaaag ctacctgtgc attattgttt tcagacatcc
    16981 attttccgta tacctgaaat accatttgcg catctgcatg gcccatctgg tttgctataa
    17041 atgccgggtt agcaccagct gtcagcgacc agcaggcata agtatgtctc gactgatatg
    17101 attttcgatg gcggagtccg gcacgtttta tcgctgcgtc ccacatctgc cttattgagt
    17161 caacggtaaa atggtcacca taatttttta ctctcgctga cacttcaggt tgaaaaacaa
    17221 aggtgcattt ttgtttttct gttctgccat actctctgag gtgaacatca atgatatgct
    17281 ctttgctcag tctcgttaat gtcatctgac tccggagagc gtcgattgct ggcttaataa
    17341 gatgaatgac ccgattggtt cccgcctgtg tttttggtac cgtgaaacgg tcttttgcta
    17401 aatttctcct gatcatcatt gttccatttt tcagatctat gtcctcccat ccaagtgcac
    17461 acagctcacc agggcgaacg ccagtataaa cagaaacaca ccataaattt tttgcttgct
    17521 gatttctgca cgcatcgata agacggataa attcctcccg cgaaagagga tccggaatgg
    17581 ttcttgattc ctttaatggc gagatcccct taaacggatt atctgccagg taaccgttat
    17641 caacaccaaa ctggaacacg gcgttaagat ttgtcatgta attatttaca gttacagccg
    17701 atctccctgg ttgtgtaaca atatagttac ttttggggat ctggtatcca gtcagtagct
    17761 ctttacgaac ctccagtaat ttttctttat taatcgatga ggcaagattt ttttcaccga
    17821 ttatgctcag gatatttttg atgacggcac ggtatgtgtt gagtgatgtt ttggcgactt
    17881 cagtttcttt cagtgccaga aatttttcag ccagttcttt tatggttaaa tcttgtcggg
    17941 cctcaccaaa tttttccaga ttgcgtgagg agggaaactg ttttgcatag tcgaaaacac
    18001 cagtttttat tgcgtaacaa acagaggagc gtagttcacc tgcaacgcgc ctgttttttg
    18061 ctgtgtcagg aacccccaga ttttccctga ctcttacgcc tttataaaca aaccagatac
    18121 gtaatttccc tccatggttt tccacgcctg tcggatattt catttcaact tctctcatta
    18181 gttagtgtgg cttttagtca agtaagatga cgtcttggtc tcgctgatgc ctggcgctca
    18241 atccagcgat caatttcttc caggttgtaa aagcatggac tgttatccca tggcataccg
    18301 tcatgagcga catgcttata ttcccttcct tccataaacg atttttcccg ggcctttttt
    18361 aacgtacctt tttttattcc tttcagcgca attaactgct cttcggatac ccatttgccg
    18421 ggagagacaa tcatgattac ttcgctcatc gatttcttta tctcttacat cagacgagcg
    18481 ccggttgcag aataccagtc acaaccggcg acagttgaac attaagaatc agcctgactc
    18541 gggatcagtt tttgccagat aactgaaacg tattttgcct ggtaacgggc gtcatcaagt
    18601 gcattatggc gctcaccttc gaatggaata gccgttctgg catcgaagtc tatggctttc
    18661 cccagctcaa cgattgtgcg tacatcgcga tcgttgtagt aacgccacgg gcaggggatc
    18721 ccctgccgtt cgtatgaacg gcgcaaaatc gtgttgtcga agttggctcc atttccccaa
    18781 acctgaacaa aaaattcacc ggagttttcg tcgataaatt cccgcaattg taacagtgca
    18841 tcatctaacg ggatttcatc ggtcataatg gcagattgcg cttcgcgtga ttgcttaagc
    18901 caccatttaa tggtgtcccg atcaatgact ccgccagcag tttccagatc gatagtctta
    18961 ctaaattccg gtcccatatc tccggtttgc ggatcgaaaa atattgcacc tattgagatg
    19021 atcggggcat caggattttt tcccatggtt tcaaggtcga tcattagatg gtcacacgtc
    19081 ctgctggtgg atgtgatttc gtgatgaccg ttcaccttaa ttgggtgatc tgccgtctcg
    19141 ccagtttcat tatcgctatt gtgatgctga ttgccgccag tgttctcctt gtgtggatgt
    19201 tcagcgcctt ccatttcctc cggatcatct tcctgaactt caacctgata ctcttcatcg
    19261 aatgtttctt ggtatgttgc gtcgcccatc accgcgccac aatcagggca gttgccgccg
    19321 ccggtctgac cgcaggcggt gcaggctttt tccacttcct gttgcgccac tggttcaggc
    19381 tgtttcgttt ctggctcgtt ttgtaacgca tttggactgt tttgttccgc tttttggtag
    19441 ttccgttccg attcatgctg gttctggttc acagaatcgc gggtctggat ccccttaacc
    19501 catttcggat cattcgggtc actaatccct tcaacaaatt caccacgtga tgcagcaagc
    19561 aacttatcgg cgtcaggctg gctgatattg gctgcctgca taattttgtt tacttcgtca
    19621 gcggtaactt ttatcggctc tggttgttct gaatcttcag cggtatctac attttgcggt
    19681 aagcccgtgt atgtgccatt ttttcgggca aaatattctt cttttgtgat ttcagtggcg
    19741 ccagcagcca gtgccttatc cagaccagaa agtttgtttg cgcgaccgta tttttctccg
    19801 tccttatctg cgaagaggaa atagaacggc ccctcacgct ctacagatgg ttcagcttcc
    19861 ggcgcggttt cattttttgg gatatcagat acctcagttt ccactgcatc agtttgtgtt
    19921 tctgatgact ggagaacatc aacagtgccc aggtctgttt cttcattctc aaacacgccc
    19981 tttgtcgtca ggtattcgca gatatatttg ttcagtgcta cgggatcttt gtgaatgtcg
    20041 atcggacgct cacggacaag gccaaaaata gtttggcggt cgtagcgaag ggcatcaggc
    20101 tgtttgcgca ttgatgccga gatacgcttc cagtcttcgc ggtcgttgtc gataacttct
    20161 ttttttgccc agcgatggat gctgccgtca atgtttccgg catccacatc accaggccag
    20221 agagcgtagg ccagttcgtc atccagtgtt ttccatgtct gcttgtattc gcgatgaatg
    20281 gcagcaatga ccgggctgat ttttcctgtt gaattttcag tgtactgttg attggctctg
    20341 gcgcgggcga gatcaacaac agacgtgtat tttccggttt ccttgcgttc accttcgcga
    20401 cgttttttcc agatgcgcat ctctgcctga atttcgggcc atttagcacc aggaatacat
    20461 ttatgcttaa cccacccaat ggcgtgcaac ttaagctccg gatacatggc gttaacttct
    20521 ggcattttca tcaacgcttc aacgatatgt ccgtcgaatg ttgccatgtc ttcctgcaac
    20581 aattcctgtg cgctaataac catatcaacg gtgatgtttt cacatgtgtc gaacttaacc
    20641 atgacagcgt tctgtacttc aggggccagc ttgtcaaaag tgacgttcat cggatcggat
    20701 tcagtctcaa ccgggacaaa agaagcagac tcctcatccc agcggttttc ctgcatatat
    20761 tcagcatccc atgaatcgag ggcagggcgg ggtatgccag gtttatcctc gcagacaata
    20821 aatttataag cgcagtcctg agcagccgga taatgttcca ggaactgcca gtgaaatttt
    20881 gctcgagcac ggcgttcgtc gccagcttca atggctgtgg ctacagccac agcgccttct
    20941 tcccttgttg ccagttcgtc aggaatagcg gcgcaaataa agactttact cattttgttt
    21001 taacctcatg acagatttaa ggatgaacaa atccctgcca ttgctggcat ataagaatga
    21061 aaccggatat ttattacgga actgttttaa agacctgccg ggatttcgat attatcctgg
    21121 tgaataactt tatcgaccgg gtaacagtta ccgggaattt tctgttcggt tgctgcagtc
    21181 atacactcct gcattgtcct gtgaacactg actgcaatat caactggcac tccggaaaca
    21241 agaaaaactg tcagaacaag cgcaaatgct gaattcattg tgcacatcct tttggcatca
    21301 gacgtaaacg agccagcatt gaaacaatgc atattttatt taatagctcc cgttcttgtt
    21361 ttctcttgtt aatggcatct tcagtaaata cagggttact gatagtgaca ccaatttcaa
    21421 aacaaccttc agacgtatta acgtttggta ataacgtttt cattatcgcg tcctcaacaa
    21481 tgaattttgt gatgcagtgc ctggtgcctc caggtgacgt taaccagtta acaattaacg
    21541 tcggatacac tacctgaggc tgtatcgcgt cgttcgctgg gattaccagc ggaaaaaatc
    21601 tgctcggtgt accgcgaaag cgacatcgta ccgggagagc agaccgccac cagcatattg
    21661 aaacaacgca caaaaaatct tgcaccgttg ccttacgccc accagcaaca aaaatcacca
    21721 caggaaaaga cggtggtaag catcaccgtt gatccagagt ctccggaatc tttcatgaag
    21781 ctgcctaaac gtcgccgctg ggttaaggag aaatacacac gttgggttaa gacacagccg
    21841 tgtgcttgct gcggtatgcc agccgacgat ccgcatcatc tgattggtca cgggcagggc
    21901 ggaatgggaa caaaagcaca tgatctcttt gtgttgcctt tgtgcagaaa gcatcacaac
    21961 gagctgcata cggatacagt ggcatttgaa gataagtatg gctcccaact ggagctgata
    22021 tttcgtttta tcgatcgcgc gctggcaatt ggcgtactgg cgtaagtgga gaacgagcat
    22081 gaaccttgaa gccttaccaa aatattactc cccaaaatct ccaaaattga gcgatgacgc
    22141 tccagcgaca ggcaccggtt gtttaacaat tacggatgta atggcagcgc aggggatggt
    22201 gcagtcgaaa gcaccacttg ggttggcctt atttctggca aaagttggtg ttcaggaccc
    22261 tcagtttgcg attgaaggcc tgctaaatta cgcgatggca ctggataacc cgacattgaa
    22321 caaattgagt gaagaaatcc ggttacagat tattccttac ctcgtgagtt ttgcctttgc
    22381 tgattactcc aggtctgcgg caagtaaggc tcgctgtgag cattgttcag gtacgggatt
    22441 ttataatgta ttgcgcgaag tggtgaaaca ctacagacgc ggggaatctg taatcaagga
    22501 agaatgggtg aaggaactat gtcagcattg ccatggtaag ggcgaagcca gcacagcgtg
    22561 cagagggtgt aagggtaaag ggattgttct ggatgaaaaa agaacccggt ttcatggcgt
    22621 accggtatat aagatttgtg ggcgttgtaa tggaaaccgg tttagtcgtt taccgaccac
    22681 gctggcacga cgtcatgtcc agaagctggt accagacctg accgattatc agtggtataa
    22741 ggggtatgcg gacgtcattg gtaaactggt aacaaagtgc tggcaggaag aagcatacgc
    22801 ggaagcgcaa ttgaggaagg tgacgagata aatgattttt gctgaagatg gcgacatgat
    22861 gtttgcattt ttcaaaaaat atggataaaa ttttttcaac gatgggcttt gtatacccga
    22921 cgttaagaaa aagtagaaaa cccgctgatg agcgggtttt gtgctttaaa tggggcaatg
    22981 gtaatgttga atctcatccc gggactcatg tctgttaact tattatttag ctggtgactt
    23041 ggttatttgc ctgatgttta aaatgttttc ttccagtaca atgtccctaa acacaatgag
    23101 tctgcttatt atattattag cagagctatt acggccaaag tacagcataa gcttttaaag
    23161 ccaatcaacc agtcatcaag acagacgggg ttattcataa aaactctcca tgtgtgatcc
    23221 gatggggcct gaaattaaag ctttaatata gctcatgaaa ggtaaacatt ggcagctgaa
    23281 gggccacgca gaccatttat ccggcaaaat tccacgcgta atccggtggt aatttcttct
    23341 gcatcgcgga gattgagcgc tgaaacatga agctggacat cgatacgacc atcggatggg
    23401 gtgataagac ccttgccgct tttgccgtca aaggttttga caattcctgt cattttacgg
    23461 gacaaaaaaa ttccttaata ctgataactt ggcgcactat acacacgttc ctgaagaaag
    23521 ctatagtttt ttgatggggt tgaagacggc tggatgtcta aaataaacat tgcttcatat
    23581 gttcaactat gcgttaatga ttgcgtcggt ttgaagaaca gacgatatac gaagtagttt
    23641 actaaagcag ttctcatttc aggtgttatt cacttattcc ttctttgagt ctctccaatt
    23701 aagtacgaag tcgtttctgt tatgcaaacc atttatgccg aaaggctcaa gttaaggaat
    23761 gtagaatgtc aaataaaatg actggtttag taaaatggtt taacgctgat aaaggtttcg
    23821 gctttatttc tcctgttgat ggtagtaaag atgtgtttgt gcatttttct gcgattcaga
    23881 atgataatta tcgaacctta tttgaaggtc aaaaggttac cttctctata gagagtggtg
    23941 ctaaaggtcc tgcagcagca aatgtcatca ttactgatta aaattcatcg ctcgtctgta
    24001 tacgataacg aagaaggctg atgcctgagt agagatacgg acagagtagt gaatattgga
    24061 tctctttaat aaaaagtaag gaggtccaat acatgaaaca atggctagca tatttggcaa
    24121 aatcttaatc aggaaaagta tgctaaccat tgtggtgaag tgcaggtttg ctgcatgaa
//
LOCUS       DAJOSY010000069        23436 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJOSY010000069 DAJOSY010000000
VERSION     DAJOSY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.069"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            73..225
                     /gene="hokA"
                     /locus_tag="OHN27_002847"
     CDS             73..225
                     /gene="hokA"
                     /locus_tag="OHN27_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCQ2287250.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIQQGSYELAAFL
                     ACNLKE"
     gene            complement(413..625)
                     /gene="cspA"
                     /locus_tag="OHN27_002848"
     CDS             complement(413..625)
                     /gene="cspA"
                     /locus_tag="OHN27_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCQ2287251.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(906..1196)
                     /locus_tag="OHN27_002849"
     CDS             complement(906..1196)
                     /locus_tag="OHN27_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCQ2287252.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1630..2340
                     /locus_tag="OHN27_002850"
     CDS             1630..2340
                     /locus_tag="OHN27_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCQ2287253.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2390..3364)
                     /gene="ghrB"
                     /locus_tag="OHN27_002851"
     CDS             complement(2390..3364)
                     /gene="ghrB"
                     /locus_tag="OHN27_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCQ2287254.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVDQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3468..4127)
                     /locus_tag="OHN27_002852"
     CDS             complement(3468..4127)
                     /locus_tag="OHN27_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCQ2287255.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4280..6613
                     /gene="bisC"
                     /locus_tag="OHN27_002853"
     CDS             4280..6613
                     /gene="bisC"
                     /locus_tag="OHN27_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCQ2287256.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6582..7022)
                     /locus_tag="OHN27_002854"
     CDS             complement(6582..7022)
                     /locus_tag="OHN27_002854"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2287257.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKVLMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7019..7582)
                     /gene="tag"
                     /locus_tag="OHN27_002855"
     CDS             complement(7019..7582)
                     /gene="tag"
                     /locus_tag="OHN27_002855"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCQ2287258.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7740..8438
                     /locus_tag="OHN27_002856"
     CDS             7740..8438
                     /locus_tag="OHN27_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCQ2287259.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGLS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8667..9875
                     /locus_tag="OHN27_002857"
     CDS             8667..9875
                     /locus_tag="OHN27_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2287260.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALLMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10199..11890
                     /gene="eptB"
                     /locus_tag="OHN27_002858"
     CDS             10199..11890
                     /gene="eptB"
                     /locus_tag="OHN27_002858"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCQ2287261.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            11982..12058
                     /locus_tag="OHN27_002859"
     tRNA            11982..12058
                     /locus_tag="OHN27_002859"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12016..12018,aa:Pro,seq:cgg)
     gene            12801..14408
                     /gene="dppA"
                     /locus_tag="OHN27_002860"
     CDS             12801..14408
                     /gene="dppA"
                     /locus_tag="OHN27_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCQ2287262.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(14471..14698)
                     /locus_tag="OHN27_002861"
     CDS             complement(14471..14698)
                     /locus_tag="OHN27_002861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287263.1"
                     /translation="MTRSLELRVCIAHKSIPALIGQGMKYCCRMALHAEAQRHRVYRL
                     LTKCALFMPDAALTPYPAYKIVQIQYIAEFS"
     gene            14716..15735
                     /gene="dppB"
                     /locus_tag="OHN27_002862"
     CDS             14716..15735
                     /gene="dppB"
                     /locus_tag="OHN27_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCQ2287264.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15745..16647
                     /gene="dppC"
                     /locus_tag="OHN27_002863"
     CDS             15745..16647
                     /gene="dppC"
                     /locus_tag="OHN27_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCQ2287265.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16658..17641
                     /gene="dppD"
                     /locus_tag="OHN27_002864"
     CDS             16658..17641
                     /gene="dppD"
                     /locus_tag="OHN27_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCQ2287266.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17638..18642
                     /gene="dppF"
                     /locus_tag="OHN27_002865"
     CDS             17638..18642
                     /gene="dppF"
                     /locus_tag="OHN27_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCQ2287267.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18672..19943)
                     /locus_tag="OHN27_002866"
     CDS             complement(18672..19943)
                     /locus_tag="OHN27_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCQ2287268.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20419..20526
                     /gene="ldrD"
                     /locus_tag="OHN27_002867"
     CDS             20419..20526
                     /gene="ldrD"
                     /locus_tag="OHN27_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCQ2287269.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(20613..22292)
                     /gene="bcsG"
                     /locus_tag="OHN27_002868"
     CDS             complement(20613..22292)
                     /gene="bcsG"
                     /locus_tag="OHN27_002868"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCQ2287270.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPLPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIEQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(22289..22480)
                     /gene="bcsF"
                     /locus_tag="OHN27_002869"
     CDS             complement(22289..22480)
                     /gene="bcsF"
                     /locus_tag="OHN27_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCQ2287271.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(22477..>23436)
                     /gene="bcsE"
                     /locus_tag="OHN27_002870"
     CDS             complement(22477..>23436)
                     /gene="bcsE"
                     /locus_tag="OHN27_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCQ2287272.1"
                     /translation="QNGIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLF
                     NNNEVLFNEARTAQAATVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLR
                     ATDERLLLACGANMVIPWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLR
                     GFQKWDVFCNAVNNMMNNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTI
                     GGNRLVLFLSFCRINDLDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPE
                     QWGMPLPLTQSSKPVINAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT         5629 a         5847 c         6176 g         5784 t
ORIGIN      
        1 tgaggctacc ctatgcctga acaccataag gttagcctct tactcgttgg aaatcaacac
       61 aggaggctgg gaatgccgca gaaatataga ttactttctt taatagtgat ttgtttcacg
      121 cttttatttt tcacctggat gataagagat tcactgtgtg aattgcatat tcaacagggg
      181 agttatgagc tggcggcatt tttagcctgc aatttaaaag agtaagagtc ttcggcggga
      241 agttattccc gcctttctta cggcgttgcg cattctcatt gcacccaaat ttatttttca
      301 caaaaataat actagatttt attacgcgat cgattattta tttcctgaaa aacaaataaa
      361 aaaatccccg ccaaatggca gggatcttag attctgtgct tttaagcaga gattacaggc
      421 tggttacgtt accagctgcc gggcctttag cgccgctttc gatggtgaag gacactttct
      481 gaccttcgtc cagagatttg taaccatcgt tctggatagc agagaagtgt acgaacacat
      541 ctttagagcc atcgtcagga gtgatgaagc cgaagccttt gtcagcgttg aaccatttta
      601 cgataccagt cattttaccg gacatagtgt attaccttta ataattaagt gtgcctttcg
      661 gcgatatggc gtgctttaca gattttgaag cgttaaagga atgtgcacta cgaggggtat
      721 caacgataac tcttgaaggg acttgcctta ctacactgct ttaatggtct gtacgtcaaa
      781 ccgttgatgt gcattaagcc acgcattggc gggtgatgca acaattattt ttcatattta
      841 tgattaatcg gttggaaaac ggtcctgtca tcaggaccgt aaacagcaat aaagtggata
      901 aaagtctatt ccatcaactg cttacttaat gccgggttgg cttgaatcaa acgcatcaat
      961 tttagttcgg cacttgaagg cttcacgcgt ctggattccc attcctttac catggcgact
     1021 gatacgccca aaacccgggc gaaatcatct atttttaatc ctgtcccttt tcgtaactgc
     1081 tcaatttcgg tacaggatgt tgttctgtgc gtcagggtaa ttttctgcgt ttcatcttta
     1141 aaaacaatct gttccaggct gctcaacagc tcatgcattg gatctttata ttccattgaa
     1201 aactcctcaa atcacactgc gggatcgtga attgcatcga agctcattaa gaatagtcgg
     1261 gaaaagcaga cacagcgggg tgaagtcagg gcatgctctg gctatgattc gattcagcca
     1321 gtcatttagc gtgacttata gccacctaac gatctgataa atgccgatat atctcttacc
     1381 actcgtggtt tgtaaagatt tcgaaaatct aaccttctgc gaaggcgtaa atctctggct
     1441 acaaaaaaag caacggcaat atcatttttt aacaatgaaa caacaaatta accttgtttc
     1501 attttctcca tgattttacc aggaagatta cgtagaaagg gtatcctgca agcctgtcct
     1561 ggactatcct tgtcacgtca gacacgcgtg tgtcgttgtg cgcttttttt gggtgaaagg
     1621 agtaagaaaa tggcgacagg aaagtcctgc tctcgctggt ttgcgcctct tgcggcgtta
     1681 ttaatggtag ttagcctgag tgggtgtttt gataaagaag gcgatcagcg taaagcgttt
     1741 atcgacttcc tgcagaatac agtgatgcgt agcggtgaac gtctaccaac cctgactgcc
     1801 gatcagaaaa aacagtttgg tccttttgtc tctgattacg cgattctgta tggttattct
     1861 cagcaggtga atcaggcgat ggattccggt ctgcgcccgg ttgtagacag cgttaacgca
     1921 attcgcgtac cgcaggatta cgttacgcaa agcggtccgc tgcgtgaaat gaatggttcg
     1981 ctgggtgtgc tggcgcaaca actgcaaaat gcgaagttgc aggccgatgc tgcgcactct
     2041 gcgctaaaac agagtgatga cctgaaaccg gtctttgatc aggcattcac caaagtggta
     2101 acgacgccag ctgatgcatt gcaaccgttg attccggcgg cacaaacttt cacgcaacaa
     2161 ctggtgatgg ttggagacta cattgcccaa caaggtactc aggtgagctt tgtagcaaat
     2221 ggcatccagt tcccgacttc acagcaggca agtgaatata acaaactgat tgcgccatta
     2281 ccggcacaac atcaggcgtt taatcaggcc tggactacag cagttactgc aacccaataa
     2341 agagtaaaag cccgagcgat tatcgggctt tactccacgc agtcgcggct tagtccgcga
     2401 cgtgcggatt cacacagttc ttctcaacct ttccttgtaa cgcatcaatc aaattatcca
     2461 cggcacaggc ggccatgcca taacgcgtct catgggtggc agatccaata tgcggtactg
     2521 cgacgacgtt ggccattgag agcaacggcg aatctacgga aagtggctct tgttcgaaga
     2581 catccagccc ggcggcgtga atttcgcctt tctgcaatgc agcgatcagt gcattttcat
     2641 caaccaccgg gccacgtccg gcattaatga atatggcgga ggatttcatt ttggcgaatt
     2701 gttctgcgcc aaatagatga tacgtctcat cagttaacgg caggatcagg caaacgaaat
     2761 ctgactcttg taacagtgta tccaaatcgc agtagcgggc gttgaagcgt tcttctgctt
     2821 ctttatggtg gcggcgcgcg ttatagagga tgggcatgtt gaagccaaag tgcgcacgtt
     2881 gtgccagcgc cataccgatc cgtcccatcc cgacaatgcc cagtgtttta tggtgaacgt
     2941 cagtgccgta ccagtccggg cctatgctcg cggtccattc gcctgctttt acccgttctg
     3001 ctacctccac aacccgacga gcggtagaca acaccagcgc catcagcgta tcggcgacgg
     3061 tttctgttaa tacggttggc gtgtgcatca gcagaatttt tcgggcggta agcgcatcga
     3121 catcaaaatt gtcatagccg acggagatcg ttgatgtggc acgcagtttc ggcatttttt
     3181 ccagcaatgc ggcatctaca ttctcgtttg aacccagtaa accttcagct tctgcaaaaa
     3241 ttgctgcatt ttgatcgacg gtttgtgggc tgaggtttgc cacctggtga acggtgaaat
     3301 gctcttgcag gcgttgcagt aaatcatcag gtaaggcttt gtagaggata acggacggct
     3361 tcatgcttct ctccattcac tgataaggcg atcaccttag cctgaaatat gatgaaggaa
     3421 aactgaagaa tgccaggtga tttctgcatc acctggcatg aaagggatta cagcgggctt
     3481 aaggtaattt ctacacggcg gttttgcgcc ttaccttctg cggtgctgtt gctggcgatt
     3541 gggttagccg ggccaaggcc ctgagtacgg atgcggctgg cgtccacgcc ctgggtgatc
     3601 aacgcgctgg caacggaatc cgcacgttgc tgggagagac gcatgttcag gtcgtgacca
     3661 cccgtgctgt cggtataacc aatcacgtta accgccgttt tcggatactc tttcagtacc
     3721 attgccacgc cggtcagggt gttagcgccc gccggtttca gggtcgcgct gctgctgtcg
     3781 aaggtcacat tgttcggcat attgaggata atgttatccc cgctgcgggt tacgctaaca
     3841 ccagtgccgc gcattttgtc gcgcagcttc gcttcctgca catccatgta ataaccaacg
     3901 ccgccgccca gagctgcgcc tgctgctgcg ccaatcagcg cgcctttacc gcgatctttc
     3961 ttcgaagaag agagcgcacc aatacccgcg cccacgagag agcccagacc tgcgccgata
     4021 gcagatttac ctgcttcgcg ttcgccggtg taagggttag ttgtgcagcc agataccgcc
     4081 agagcgccac tcactacggc ggcaataaga taaacacgtt tcttcattgt taatccttaa
     4141 taaccttttt attctttgcc acgggttccg tggcgggaga ttatgccgcg tgaacatgaa
     4201 gattattcct gggaatactc ggaaatttgt aagtaatatt taactgctca atacatctaa
     4261 cctttcagga gtcttcggtt tggccaactc atcctcacga tattccgttc tgactgccgc
     4321 ccactggggg cccatgctgg ttgaaaccga cggcgaaacc gtgtttagct cgcgtggcgc
     4381 gttagccaca ggaatggaaa actccttgca gagcgcggtt cgcgaccagg ttcacagcaa
     4441 tacgcgggta cgatttccaa tggtgcgcaa aggctttctc gcgtcaccgg aaaacccgca
     4501 aggcattcgt gggcaggatg aatttgttcg cgtgagttgg gatgaggcgc tggatcttat
     4561 tcaccaacaa cataaacgca ttcgtgaggc ttatggtccg gcatcgattt ttgctggttc
     4621 ctacggctgg cgttcaaacg gtgtgctgca taaggcctcg acattattac aacgctatat
     4681 ggcgctggca ggcggttata ccgggcatct gggggattat tcgaccggcg cggcgcaggc
     4741 gatcatgccg tatgtcgtgg ggggcagcga agtttatcaa cagcaaacca gttggccgct
     4801 ggtgctggaa catagcgatg tcgtggtgtt gtggagtgct aacccactca atacgctgaa
     4861 aattgcgtgg aatgcatctg atgagcaggg gctttcttac ttttccgcac tgcgtgacag
     4921 cgggaaaaag ctgatctgta ttgatccaat gcgatcggaa accgtcgatt tctttggcga
     4981 taaaatggag tgggtggcac cgcacatggg caccgatgtt gcgctgatgc tggggatcgc
     5041 ccatacgctg gtggaaaatg gttggcacga cgaagcgttt ctggcgcgtt gcaccacagg
     5101 ttatgccgtc ttcgcctctt atttgctggg cgagagtgac ggaatagcga aaaacgccga
     5161 atgggcagca gagatttgtg gtgttggcgc agcgaaaatc cgcgagctgg cggctatttt
     5221 ccaccaaaat accaccatgc tgatggcagg ctggggaatg cagcgccaac agtttggtga
     5281 gcaaaaacac tggatgatcg tcacgctggc agcaatgttg gggcaaatcg gcacacccgg
     5341 cggcggtttt ggtctttctt accattttgc caatggtggt aaccccacgc ggcgttctgc
     5401 ggtgctctct tccatgcagg gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc
     5461 tgttgcccgc attgttgaag cactggaaaa ccctggtggc gcatatcaac acaacggtat
     5521 gaaccgacat ttcccggata ttcgttttat ctggtgggcg ggcggtgcca actttactca
     5581 tcatcaggat accaatcgtc tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc
     5641 tgaatgcttc tggacggcgg cggcgaaaca cgcggatatc gttctgcctg cgactacctc
     5701 ttttgagcgt aatgatctca ccatgaccgg tgattacagt aatcagcatc tggtgccgat
     5761 gaagcaagtg gtgccgccac gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt
     5821 gagtgaacgt tgggagaagg gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca
     5881 atggctggaa acgttttata acgttgcccg gcagcgcggg gcaagccagc aggttgaatt
     5941 accgccattt gctgagttct ggcaagccaa ccagttaatt gagatgccgg aaaacccgga
     6001 cagcgagcgg tttattcgct tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa
     6061 aaccgccagc ggcaagattg aaatcttctc acagcgtatt gccgattacg gttacccgga
     6121 ttgccctggg catccaatgt ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga
     6181 acagttgcag gtactttctg cccatccggc gcaccgcctg cacagccagc tgaattacag
     6241 ttctctgcgc gaattgtacg cggtagcaaa ccgtgagcct gtcaccattc atcctgacga
     6301 tgctcaggcg cgcggcattc aagatggcga tactgttcgg ctgtggaacg cacgcgggca
     6361 aattcttgcc ggagcggtca ttagcgaggg aattaaacct ggcgtgattt gtattcacga
     6421 aggggcatgg ccggatttgg atttaaccgc tgacggtatt tgtaaaaacg gcgcagtcaa
     6481 cgtgctgacc aaagatctcc ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc
     6541 gctggcatgg ctggaaaaat acaacggtcc ggaactgaca cttacagcgt ttgaaccacc
     6601 ggccagctca taatccaggt gggtagttgg gtttcatcct gccatgcgca atcgacaatg
     6661 tgaaaaccct gcgcacggta aaaatctatc gccggttgat ttttttgata aacctccagc
     6721 atcagatggg gatagcgctg ctgcacatac tgcatcagca ccttaccaat accgcgtctg
     6781 acggccttcg gtgcgacaaa cattgctgcc agaaatcggc cttccataat gctgacaaaa
     6841 ccgagaagct taccgtcctc ttcccagacc cagttttgcg cattggcaag ataggcatcc
     6901 cgcaccagcg gaatgcagtc acgccagtaa ttcgctttta taaagggatg cccccaggtt
     6961 gtactttcca gccacagttc gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc
     7021 atggtttatt tcccggatag caacagcagc caaccacatg atcattcacc agcccacatg
     7081 cctgcataaa ggagtaacag attgtggtgc cgacaaactt aaaaccacgt tttttcagtg
     7141 ccttagatag ggcgtcggag gcggacgtag atgtgggaat ttcgctcaac gttgtggctt
     7201 gtgtcacctg tggctgatga tttacaaacg accagacaaa gtcggcaaac ggttcgccgt
     7261 tctgttccat ttgcaggtac gcccgcgcat taccaataat tgcctgaatt ttccctcgat
     7321 ggcggataat cccggcgtcc tgtaccagtc tttcgacatc ctcttcctgc attgcggcaa
     7381 ccttcaccgg atcgaactga tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg
     7441 tgatccacga taacccagcc tgctgccctt caaggcagat catttcgaac agttttttac
     7501 tgtcagtttc aggcacgccc cactcattat catggtaggc aatataaagc gggtcctgac
     7561 tcacccagcc gcaacgttcc atactttccc tcgctatgta cccgatcgta aaaatttcag
     7621 ccgcttttct tgacgcatcg gtgaaaaagt caatatttaa tacagggcta ctatgcccga
     7681 tatccttcat acaatgacaa taatattgcc gaattcggct cttccctgga gtcgtgttaa
     7741 tgatcataaa aaaaagcggt ggtcgctggc agctaagcct gctggcgagc gtggtaatca
     7801 gtgccttttt tctcaacaca gcttacgcct ggcaacaaga atatatcgtt gatacgcaac
     7861 ccggactttc cacagagcgt tacacctggg atagtgatca tcaacctgat tacaacgata
     7921 ttttgtcgca acgtattcaa agtagccaaa gggcgctggg actggaagtc aatctggcgg
     7981 aagaaacccc tgtggatgtg accagcagta tgagtatggg ctggaatttt cctttatatg
     8041 aacaggttac aaccggcccg gtcgcggcat tacattacga tggcacaacc acctcgatgt
     8101 ataacgagtt tggcgacagt actaccacgc tgaccgatcc gttatggcat gccagcgtga
     8161 gtaccttagg ctggcgtgtt gactcccggc ttggcgatct ccgaccctgg gcgcaaatca
     8221 gctataacca gcaatttggc gagaatatct ggaaggcgca atcaggcctg agccggatga
     8281 cggcgacaaa ccagaacggc aactggctgg atgtcaccgt aggcgctgat atgttgctca
     8341 atcaaaatat tgccgcctat gccgcgctaa ctcaggcaga aaataccact aataatagcg
     8401 actatctgta tacgatgggg gttagcgcca gattttaacg taacagtcac aattgaaacc
     8461 attaaataac aatagttgtg gcgatagtgg gtgctaactt accaaataat aaatttggtg
     8521 aataattgtc gcgtcattca ttcctgaact aaggcatttc attccgttct gatggcattt
     8581 catgccgttt ttccccaggc ataaagtgca cttcgttatg gttgtcggca gagatttttc
     8641 ctttttatta ctgcaggaat actgccatga caccttcaaa ttatcagcgt acccgctggc
     8701 tgacactcat cggtactatc attacccagt ttgcgctggg gtcggtttat acctggagcc
     8761 tgtttaatgg cgcgctttcc gccaagctgg atgcgccggt aagccaggtc gctttctctt
     8821 tcggcttgtt aagtctgggg ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac
     8881 gttttggcgt taaacgcgtc accatggctt ccggcatttt gctgggatta ggcttcttcc
     8941 tgacagcgca ttctgacaac ctgatgatgc tgtggttaag cgccggtgtg ctggtgggac
     9001 tggcagatgg cgcgggttat ctgctgacgc tctctaactg tgtgaagtgg ttcccggagc
     9061 gtaaaggtct gatctccgcg ttcgctatcg gttcttatgg tctgggtagc ctgggtttca
     9121 aatttatcga cacgcagctg ctggaaacgg tcggtctgga aaaaaccttt gtgatttggg
     9181 gagcgattgc gctgttgatg attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac
     9241 aggaagtgaa aaccagcaat ggtgtggtgg agaaagatta cacgctggca gagtcgatgc
     9301 gtaaaccgca gtactggatg ttagcggtaa tgttcctgac cgcctgcatg agcggcctgt
     9361 acgtgattgg ggtagcgaaa gatatcgccc aaagtctggc acaccttgat gtggtttccg
     9421 cagccaatgc agtcactgtt atttccatcg ccaacctttc aggtcgtctg gtgctgggta
     9481 ttctgtctga caaaatcgcc cgtatccgtg ttattaccat tggtcaggtg atatcgctgg
     9541 tgggtatggc ggccctgctg tttgcaccat tgaatgcagt gacgttcttt gcagcgattg
     9601 cctgcgtggc atttaacttt ggcggcacta ttaccgtctt tccgtcactg gtcagtgagt
     9661 tctttggcct caataacctg gcgaaaaact acggtgtgat ttatctcggt ttcggtatcg
     9721 gtagcatttg tggttcgatt atcgcctcac tgtttggcgg cttctatgtg accttctacg
     9781 tgattttcgc cctgctgatt ctgtcattgg cgctttctac gacgattcgt cagccagagc
     9841 agaaaatgtt gcgtgaggcg catggctccc tttaatgctc agccgcgagc tactgggctt
     9901 cagcttccgt gtgcatcaaa cgcatcaacg aaatgtataa acaatgactc gtaaaatttg
     9961 ggccaattaa ctggcccttt ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg
    10021 catttttgta aatttgtgct tcatgcacac tctttcccca cactttttcc ctttgctgtg
    10081 gtctacttat tcgcgcgtgt agattttact tatctgacta cctccgcact ttttccctgc
    10141 cgggcctgaa aagccactaa gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat
    10201 gagatacatc aaatcgatta cacagcagaa gctgagcttt ttgcttgcaa tctatattgg
    10261 cctttttatg aatggcgcgg ttttttaccg ccgcttcggc agctatgcgc acgattttac
    10321 cgtctggaaa ggcatttctg ctgttgttga actggccgcc accgtactgg tgaccttctt
    10381 tttactacgt cttctttcgc tgtttggccg ccgcagctgg cgtattctgg catcgctggt
    10441 ggtgctcttt tccgcaggtg ccagctatta catgaccttc cttaatgtgg tcattggtta
    10501 tggcatcatc gcttccgtca tgaccaccga tatcgacctg tcaaaagaag ttgttggtct
    10561 gaactttatt ctctggttaa tcgccgttag tgcattgcct cttatcctta tctggaataa
    10621 ccgctgtcgc tacaccttgc tccgacaact gcgaacccca gggcagcgta ttcgcagcct
    10681 ggcggtcgtc gtactggcgg gtattatggt ttgggcaccg attcgtttgc tggatatcca
    10741 gcagaagaaa gtggagaggg cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc
    10801 gaactcttat ctgccatcaa actggctttc tgcgttgggg ctgtatgcct gggcgcgggt
    10861 cgatgaatct tccgataata attcattgct taatccggcg aagaaattca cttatcaggc
    10921 accgcaaaac gttgatgaca cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga
    10981 ccatatgggt attttcggct atgagcgtaa taccacgccg aaactggccc aggagaaaaa
    11041 tctggcggcg ttccgtggtt actcctgtga taccgcaacc aaactctcac tgcgttgcat
    11101 gtttgtacgt caggggggcg cggaagataa tccgcagcgc acattaaaag aacagaacat
    11161 tttcgcggtt ctgaagcagt taggattcag ttctgacctc tacgctatgc agagcgaaat
    11221 gtggttctac agcaacacga tggcggacaa cattgcttat cgtgagcaga ttggtgcgga
    11281 gccacgtaat cgtggcaagc cggtagatga tatgttgctg gtagacgaaa tgcagcaatc
    11341 gctagggcgc aacccggatg gtaagcatct gatcattctg cataccaaag gttcgcattt
    11401 taactacacc cagcgttatc cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt
    11461 tgatagcggc tgtaccaaag cgcagatgat caactcctat gacaactcgg tgacctatgt
    11521 ggatcacttt atctccagcg tgattgatca ggttcgcgat aagaaagcga ttgtgttcta
    11581 cgcagctgac cacggtgagt caattaatga acgcgagcac ctgcacggca cgccgcgtga
    11641 actggcaccg ccggagcagt tccgcgtacc gatgatggtc tggatgtcag ataaatatct
    11701 ggaaaatccg gccaatgcgc aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt
    11761 gccacgccgt cacgtagagc tgtacgatac catcatgggt tgtcttggct atacttcacc
    11821 ggatggtgga attaacgaaa acaacaactg gtgtcacatc ccgcaggcaa aagaggcagc
    11881 ggctaactaa gcggcctgct gactttctcg ccgatcaaaa ggcattttgc tattaaggga
    11941 ttgacgaggg cgtatctgcg cagtaagatg cgccccgcat tcggtgattg gcgcagcctg
    12001 gtagcgcact tcgttcggga cgaaggggtc ggaggttcga atcctctatc accgaccaaa
    12061 ttcgaaaagc ctgctcaacg agcaggcttt tttgcatctg cagctcatga ggatgagaac
    12121 ctccgggggc agggaggttc gactcgacgt aggcctgata agacgcgtca gcgtcgcatc
    12181 aggcaaatgt gccggccatt gtcggatgcg gcgcgaccgc cttattcgac ctacacgaca
    12241 cacatttccc tgccaaattt gcgccccata tctcactatc cattaacgga tttgtgacag
    12301 aaaccattgt ctttttttat atttgcttaa atcttttttt gcgttactta tggctggccc
    12361 tggcattttc cgctgtgcgt tttagcgttc atggtattaa tcgctcagat atccaccttt
    12421 tcctcagatt tttttcatta aatgcataaa acttaatcac caaatgttgc taaatattaa
    12481 gctgattgtt ctggtcatgt tggagtagca caaatttctc tgctgcaaat agctgtttga
    12541 agttttttta atctttgttt gtggattctc accgttggtg taaatcccgg ttggctgtat
    12601 tgacgttttc acattctgtt gacagattgt aggtcacgag gggcatttta tggaggatcc
    12661 gcactgttac actgatgtta attagtacgg catccccacc tcataacgtt gacccgaccg
    12721 ggcaaaaaac aaaaaaggtc aggcagcgac aacccactgc aaagggttaa aacaacaaac
    12781 atcacaattg gagcagaata atgcgtattt ccttgaaaaa gtcagggatg ctgaagcttg
    12841 gtctcagcct ggtggctatg accgtcgcag caagtgttca ggctaaaact ctggtttatt
    12901 gctcagaagg atctccggaa gggtttaacc cgcagctgtt tacctccggc accacctatg
    12961 acgcctcttc cgtcccgctt tataaccgtc tggttgaatt taaaatcggc accaccgaag
    13021 tgatcccggg cctcgctgaa aagtgggaag tcagcgaaga cggtaaaacc tataccttcc
    13081 atctgcgtaa aggtgtgaag tggcacgaca ataaagaatt caaaccgacg cgtgaactga
    13141 acgccgatga tgtggtgttc tcgttcgatc gtcagaaaaa cgcgcaaaac ccgtaccata
    13201 aagtttctgg cggcagctac gaatacttcg aaggcatggg cttgccagag ctgatcagtg
    13261 aagtgaaaaa ggtggacgac aacaccgttc agtttgtgct gactcgcccg gaagcgccgt
    13321 tcctcgctga cctggcaatg gacttcgcct ctattctgtc aaaagaatat gctgatgcga
    13381 tgatgaaagc cggtacaccg gaaaaactgg acctcaaccc aatcggaacc ggtccgttcc
    13441 agttacagca gtatcaaaaa gattcccgta tccgctacaa agcgtttgat ggctactggg
    13501 gcaccaaacc gcagatcgat acgctggttt tctctattac ccctgacgct tccgtgcgtt
    13561 acgcgaaatt gcagaagaat gaatgccagg tgatgccgta cccgaacccg gcagatatcg
    13621 ctcgcatgaa gcaggataaa tccatcaatc tgatggaaat gccggggctg aacgtcggtt
    13681 atctctcgta taacgtgcag aaaaaaccac tcgatgacgt gaaagttcgc caggctctga
    13741 cctacgcggt gaacaaagac gcgatcatca aagcggttta tcagggcgcg ggcgtatcag
    13801 cgaaaaacct gatcccgcca accatgtggg gctataacga cgacgttcag gactacacct
    13861 acgatcctga aaaagcgaaa gccttgctga aagaagcggg tctggaaaaa ggtttctcca
    13921 tcgacctgtg ggcgatgccg gtacaacgtc cgtataaccc gaacgctcgc cgcatggcgg
    13981 agatgattca ggcagactgg gcgaaagtcg gcgtgcaggc caaaattgtc acctacgaat
    14041 ggggtgagta cctcaagcgt gcgaaagatg gcgagcacca gacggtaatg atgggctgga
    14101 ctggcgataa cggggatccg gataacttct tcgccaccct gttcagctgc gccgcctctg
    14161 aacaaggctc caactactca aaatggtgct acaaaccgtt tgaagatctg attcaaccgg
    14221 cgcgtgctac cgacgaccac aataaacgcg ttgaactgta caaacaagcg caggtggtga
    14281 tgcacgatca ggctccggca ctgatcatcg ctcactccac cgtgtttgaa ccggtacgta
    14341 aagaagttaa aggctatgtg gttgatccat taggcaaaca tcacttcgaa aacgtctcta
    14401 tcgaataatt aaaagccata caagactgat ggcaaaggca aaaatgcctg atgcgctccg
    14461 cttatcaggc ctacgaaaat tctgcaatgt attgaatttg cacgattttg taggccggat
    14521 aaggcgttaa cgccgcatcc ggcataaaca aagcgcactt tgtcaacaat ctgtataccc
    14581 ggtggcgctg tgcctctgcg tgcagcgcca tccggcagca atacttcatt ccctgtccga
    14641 taagggcggg aattgatttg tgagcaatac agacacgcag ttccaggctg cgggtcacta
    14701 tagagaatcc gggttatgtt gcagtttatt ctccgacgtt tgggactcgt catccccacg
    14761 tttatcggta ttacccttct cacatttgcc tttgtccaca tgatcccggg cgatccggtg
    14821 atgatcatgg cgggcgaacg tgggatctcc ccagagcgtc acgcgcagct gctggctgaa
    14881 ctcggcttag ataaaccgat gtggcagcag tatctccatt acatttgggg cgttatgcat
    14941 ggcgatctag gcatttcaat gaaaagccgc atcccggttt gggaagagtt cgtgccgcgc
    15001 ttccaggcca cgctggaact tggcgtctgc gcgatgattt ttgctacggc agtcggtatt
    15061 ccggtcggcg tgctggctgc ggttaaacgc ggttccattt tcgatcacac agcggttggc
    15121 ctggcgctga caggttattc aatgcctatc ttctggtggg gcatgatgct gatcatgctg
    15181 gtttcggtgc actggaacct gacgcccgtc tccggtcgcg tgagcgatat ggtgttcctc
    15241 gatgactcca atccgttaac cggttttatg ctaatcgaca ccgccatctg gggtgaagac
    15301 ggcaacttta tcgatgccgt cgcccatatg atcttgcctg ccattgtgct gggtactatt
    15361 ccgctggcgg tcattgtgcg tatgacacgc tcctcgatgc tggaagtgct gggcgaggat
    15421 tacatccgca ccgcgcgcgc caaagggcta acccgcatgc gggtgattat cgtccatgcg
    15481 ctgcgtaacg cgatgctgcc ggtggtgacc gttatcggcc tgcaggtggg aacattgctg
    15541 gcgggggcga ttctgaccga aaccatcttc tcgtggcccg gtctgggacg ctggttgatt
    15601 gacgcactgc aacgccgcga ctatccggta gtgcagggcg gcgtattgct ggtggcgacg
    15661 atgattatcc tcgtcaactt gctggtcgat ctgctgtacg gcgtggtgaa cccgcgtatt
    15721 cgtcataaga agtaagggga catcatgtca caggttactg aaaataaagt gattagcgca
    15781 ccggtgccga tgaccccgtt acaggagttc tggcactatt ttaaacgcaa caaaggcgcg
    15841 gtcgtcgggc tggtttacgt cgtcatcgtg ctgttcatcg cgatctttgc caactggatt
    15901 gcaccctata acccggcgga acagttccgc gatgcactgc tcgccccgcc agcctggcag
    15961 gaaggcggca gcatggcgca cttgctgggc accgatgacg taggccgtga tgtgctgtcg
    16021 cgcctgatgt acggtgcgcg cctgtcgctg ctggttggct gtctggtagt tgtgttatcg
    16081 ctgattatgg gcgttattct cggcctgatc gccggttact ttggcggcct ggtcgataac
    16141 atcattatgc gcgtggtcga tatcatgctg gcgctgccaa gtctgctgct ggcgctggtg
    16201 ctggtggcaa ttttcggccc gtcgattggt aacgccgcgc tggcactgac cttcgttgcc
    16261 ttgccgcact atgtgcgctt aacccgcgcc gccgtgctgg tggaagttaa ccgcgattac
    16321 gtcaccgcgt ctcgcgtggc gggtgccggg gcgatgcgtc agatgtttat taacatcttc
    16381 ccgaactgcc ttgcgccgct gattgttcag gcgtcgctcg gtttctctaa cgccattctc
    16441 gatatggctg ctcttggttt cctcggcatg ggggcacagc cgccaacgcc tgagtggggc
    16501 accatgctct ccgacgtgtt gcagttcgcg caaagcgcct ggtgggtcgt gaccttcccg
    16561 ggtctggcga tcctgctgac ggtgctggca tttaacctga tgggtgacgg tctgcgtgac
    16621 gcgctcgatc ccaaactgaa gcagtaagag gttcgagatg gcgttattaa atgtagataa
    16681 attatcggtg catttcggcg acgaaagcgc gccgttccgc gccgtagacc gcatcagcta
    16741 cagcgtaaaa cagggcgaag tggtcgggat tgtgggtgag tccggctccg gtaagtcggt
    16801 cagttcactg gcgattatgg ggctgattga ttatccgggc cgcgtaatgg cagaaaaact
    16861 ggagtttaac ggccaggatt tgcagcgtat ctcagaaaaa gagcgccgca acctggtggg
    16921 tgccgaagtg gcgatgatct tccaggaccc gatgaccagc cttaacccgt gctacaccgt
    16981 gggtttccag attatggaag cgattaaggt gcatcagggc ggcaacaaaa gtacccgccg
    17041 tcagcgagcg attgacctgc tgaatcaggt cggtattccc gatccggcat cgcgtctgga
    17101 tgtttacccg catcagcttt ccggcggcat gagccagcgc gtgatgatcg ccatggcgat
    17161 tgcctgtcgg ccaaaactgc tgattgccga tgaaccgacc accgcgctgg acgtgaccat
    17221 tcaggcgcaa atcatcgaac tactgctgga gctacagcag aaagagaaca tggcgctggt
    17281 gttaattacc catgacctgg cgctggtggc ggaagcggca cataaaatca tcgtgatgta
    17341 tgcaggccag gtggtggaaa ccggtgatgc gcacgccatc ttccatgcgc cgcgtcaccc
    17401 gtatactcag gcattgctgc gtgcgctgcc agaatttgct caggacaaag aacgtctggc
    17461 gtcgttgcca ggtgtcgttc ccggcaagta cgaccgcccg aacggctgcc tgcttaaccc
    17521 gcgctgcccc tatgccactg acagatgtcg cgctgaagaa ccggcgctga atatgctcgc
    17581 tgacgggcgt cagtccaaat gccattaccc acttgatgat gccgggaggc cgacactatg
    17641 agtacgcaag aggccaccct gcaacaaccg ctgttgcagg ctatcgacct gaaaaaacat
    17701 tatccggtga agaaaggcat gttcgcgccg gaacgtctgg ttaaagcgct ggatggcgtt
    17761 tcgtttaacc ttgaacgtgg caaaacgctg gcagtagtgg gcgaatctgg ctgcggtaaa
    17821 tcgaccctcg gtcggttgct gacgatgatt gaaatgccca ccggtggcga gctgtattac
    17881 caggggcagg atctgcttaa gcacgatccg caggcgcaga agctgcgtcg gcagaaaatc
    17941 cagatcgtct tccagaaccc ttacggttcg ctgaatccgc gtaaaaaagt cgggcaaatt
    18001 cttgaagagc cgctgctgat caacaccagc ttaagcaaag aacagcgtcg ggaaaaagcc
    18061 ctgtcgatga tggcgaaagt cggcctgaaa accgagcact atgaccgcta tccgcatatg
    18121 ttctccggcg gtcagcgtca gcgtatcgcc atcgcccgtg gtctgatgct cgacccggat
    18181 gtggtgattg ccgatgaacc ggtttccgcg ctggatgttt cagtgcgcgc gcaggtgctg
    18241 aatctgatga tggatttgca gcaggagttg gggctgtctt atgtctttat ctcccacgac
    18301 ctgtcggtgg tggagcacat tgctgatgaa gtgatggtga tgtacctggg ccgctgcgtg
    18361 gagaagggaa cgaaagacca aatcttcaat aacccgcgcc atccgtacac tcaggcgcta
    18421 ctttccgcga cgccgcgcct gaacccggac gatcgccgcg agcgcatcaa gctcagcggt
    18481 gaactaccaa gcccactgaa tccaccgccg ggttgcgcct tcaacgcccg ctgtcgtcgg
    18541 cgcttcggcc cctgcaccca gttgcagccg cagctaaaag actacggcgg tcaactggta
    18601 gcttgttttg cggttgatca ggatgaaaat ccgcagcgtt aactcacaaa accggggcca
    18661 tgcgctccgg tttactcaat cagcttcaac agtggcgaca agcatagcaa aatgccgtac
    18721 agcagaatca gccaggcttt aaatccgcga agtttttcca gttgtgcgac tttatagatc
    18781 aggaaaaacg gaatgaggca cgacacaata ccatataacg ggcttcccaa ctgaaagaac
    18841 accagcaccg atacacgaaa cgaaacccaa atcgtcaacg taatgacgat aaaagcgcag
    18901 atcgccagag tcagcacgcg tgagttaatt ttcttggtat caataattcg gcttaacaga
    18961 ttgagaataa tgcctttaat ggcctcgtgg aaaccgagat aaatgccaaa gaatgcggtc
    19021 agtacggcaa agatattaag caccgtagag gtgatatgaa tgatatgccc agggatcacc
    19081 tgcgcggcca gcgccagtgc tgagatattt tgttcaaagg cagaaacggc ttcttcgtgg
    19141 ctaattgaga aggtaaacga aaaggcaaaa aacaggatca ccgcgatgag cgtgatataa
    19201 ctaattcggt gggtacgcag cgcgagccgg gttgccagta ctttatccgc ttcccgttta
    19261 cgataggcaa tattcattgg gttaagtacc tgaataaata ctgcagaaaa gaagcaaaat
    19321 ggaatggtaa gcaagacatc gcggaaaaag acggaggctt gcgggaaggc ggttatattg
    19381 gcgaaattcc agtgcgggat catcgcaaaa ccgaacacga caataatccc tactttgacc
    19441 accaccattg gcccggaaat cttaaacagt aatcgttcac caccagacgc aatcgccacc
    19501 agtacggcga aaatagcgac tttataaagt agagattgtg aaagatcggc atcggttaaa
    19561 ccgaaggttt tcaggtacga ggcgctgtcg aaaaccacgg agagagagta gataaaaatc
    19621 ccgtggataa tcatcaaaaa gtagataacc ccgaggaaaa ttccccagtt cttccccagg
    19681 taatgactga taatatcggt gtagtcatta caggaatcgc tttcagaaag ggtttttaaa
    19741 taaatgtcct gcactaccca ggtggcagga taagcaatga tcgctgcggt aataaatacc
    19801 caaattccct tcaagccaat ttgtactggc atcagcacgg ttccggcacc aatcgccatg
    19861 cctatgcata ataaaaccca gccgaagtcg tagcgtgtaa acggcaattt ctgattgtgt
    19921 ttcgatagtg tgttgtgctg cataaagtcc tgtccagaaa gaagtcggcc tcgcaaaaat
    19981 gaataaaccc agtttctctt gcgcaacagg tggagaagtc gaatacgcag tatgtaaaaa
    20041 atgaggcaac agattcactg gggaggtcgc gaggaggcgc aattctacaa aattgcgcat
    20101 ttcactacca gtctggagtc agcccggata tgaactgcaa ctattgcaaa taaagaaatt
    20161 aagctggaag gcatcgcgca atgaggaaga ttgccgggtg ctgacagtaa aaagcaatcg
    20221 tagaatcagc ggcaagccgg aaaggttccg gtgaggcgca atgttgcggg ggctttatcc
    20281 ctggtggcat tggttgctgg agagagaaaa cccccgcacg ttgcaggtat gcacctgaca
    20341 acaccacggg ggctaatctt gactctagac cactcaagaa tagccgcgaa acgttgtcat
    20401 tacaacacag gcggctatat gacgttcgca gagctgggca tggccttctg gcatgattta
    20461 gcggctccgg tcattgctgg cattcttgcc agtatgatcg tgaactggct gaacaagcgg
    20521 aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca atttgcgccc ggaccaggcc
    20581 gcagggggga aactctgcgg cctttttcgt tcttactgcg ggtaaggcac ccagtcgccg
    20641 ccgttcaggc gaacgtacgg tttatcctgg tattgaataa ctactgcatt tgagttctcg
    20701 gagaccggtg ctgtttgtgg caacccactg gtgagttttt tccagtcaac attgtcttcg
    20761 gtgaaaatct tgccatcgag aacgcgaacc accagatcgg agatagccag gaagctgctc
    20821 ggttgttcga tgacaatcgg tgccccctga tgcggtgcct tcatgccgaa gaatttcacc
    20881 ccaacgggga cgtcggtgat agacgggcta gggatatcac gtaggccaga tacctgcatt
    20941 ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga ccaccatcac tttacgaccc
    21001 gatttctcaa gttcagtaaa gaaggcgtcc agttcatcaa agaatttctg cgcccgcgct
    21061 ttgtaatccg ctgttttgct gacccccgga taatggttgc cgtcatgcag tggaagcgtg
    21121 ttgtagaacg tggcactacg gctgttttta tctttttcgg taacgtccag ccagcggtta
    21181 agcacagcgg tatcgtcata aaccggcgaa ccatcaaagc ccagcaaaat aaccggcaga
    21241 tttgtttgat ccatcaattc gctctgcatg ccgccatttt cgcgaacttc tttcaaaaaa
    21301 ccgccgaact ggccgttatg tcccatcatc aggtgctggg taaagcccag tttcgaaagg
    21361 ttatcaaaca gatagcagtc gttatttgcc ggttgataca gattagtgtg cgaagtctgc
    21421 ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac tgtaggaggt ggcggagtta
    21481 aagttcttga actcaatatc gaaatgcgac cacagtggat gcgacatcaa cccggcggct
    21541 tctatatccg accaggaaag cgaacagatg ttaatcacca gtagttcaaa tggctgagca
    21601 tcagcgggca gcgaagacgg gaaggtcgat ttacgtttcg cctccgcgtt atagaaatta
    21661 ttcagccagg cgttaaggtt cgccgttgtt ggcggtgcag tttgtgcggg catatcaccc
    21721 actaccggtg cgccacccgt cgccgcaacg gttgccgctg cgttaccacc cgtcgttgtt
    21781 acagtggtcg tcggttgtcc ggctggccac aaggagaaac ttggtcccgc cagggtaagt
    21841 acgttcagcc atagcagtat ggcaaccaca aaaacggtaa tgcgaatcca ttgtgacagg
    21901 aataaccagg ccactaataa aacaaaaatg gccccaatca tctgccagtt aataaagcgt
    21961 gtgacaaggt cgattaaata atcggtactg aaccccgcca cctgcgaacc ctggctcatt
    22021 atgctttccg ggccaggcaa ccaggtgtca tgccagaaca aagcaaagcc gatcggcagg
    22081 gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa ggggcatcag cagaaacgcg
    22141 gcaaacacca aattgaggag cggatggaag ttaagatatc ccgcccacaa caggccgaac
    22201 ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc agtattgcca gagggaagaa
    22261 ggcatggcgg tattttgcgt aaattgagtc attttttggt tgccctggct ttttccgtgc
    22321 ggcgtaacgt cccggccggt ttaacataac gaggtttagc aaagaacaaa cgtaaggtgt
    22381 cgcgaatgcg tcgcaaagag tgccgcgcca gatagcccag cgggaaaaat atcagtgcgc
    22441 aaacgacaat aatttcaatg atatcgctga tggtcatcat gatgagcgct ccacagcatc
    22501 atctaacagt cgcatgggtt ctggtattcg tcgccagtgg cgaccatcgt gctcggcatt
    22561 gatgaccggt ttagaacttt gcgttaaagg cagcggcatg ccccattgtt ctggggcaag
    22621 caagcgcatc tgcaccagct cggcactgat ttgatcatct tcaaaccaga ccatacggtt
    22681 tgagaaaatg tcgccagtag gcaatgggaa aatatgattc aacgcggtat ccagatcgtt
    22741 aatccgacag aatgagagaa acagcaccag ccgattaccg ccaatggtca tgatatcgcc
    22801 ggtacggtta gggcgacaca gcgtcagggc ttgttcaacg cggatacccg gtaccggacg
    22861 tagggcaacc agaacgcctt taccgtgggc aggtaataga gggttattca tcatgttgtt
    22921 gacggcatta cagaacacat cccacttctg gaaaccacgc agtttgagcg gctgggtcat
    22981 tgacagcaag gtagtgatat cttccggcac atagcgacta aacttctgcc cttgcacgct
    23041 ttcgatcatc gtcagacaac gggagagtgg cgcattccac ggaataacca tatttgcacc
    23101 gcaggccaat aacaaacgtt catcggtggc gcgcaggcta gcggtatttt cccgcacgag
    23161 gattttcatc gcactaccgc gctggcgacg cagggtatga atgctgcggg ccagtggctc
    23221 gatttgcgca ttttgctgta aagaaaagac caccgtcgcc gcctgagcgg tacgggcttc
    23281 attgaacagg acttcgttat tgttgaacag ttgccagtgt tccgatagcg gcggcgcacc
    23341 ttccagtaca gcaacattac tcagaatgcg tttttcgtcg ctgcgtggtt ggatctccgc
    23401 ctcttcgctt tgaactaatg tccagatacc attttg
//
LOCUS       DAJOSY010000070        23184 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJOSY010000070 DAJOSY010000000
VERSION     DAJOSY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.070"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1025
                     /locus_tag="OHN27_002871"
     CDS             <1..1025
                     /locus_tag="OHN27_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCQ2287273.1"
                     /translation="LLVAGAANAAEIYNKDGNKLDLYGKMVGERILTDRENGEKGDNS
                     QDTSYARVGVKGETQINPELTGYGQFELDLEASNRHNPDQTRLAYAGLSYKDFGSFDY
                     GRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTDFFGMVEGLNFAL
                     QYQGKNEGTGNYKANGDGHGLSVTYTIDGFSFTGAYANSDRTDFQSSDSKGEKAEVWA
                     LSTKYDPNNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDFGLRPSIGYSYSK
                     ALDVAGYKDSDRLNYIEIGTWYYFNKNMNVYTAYQINLLDKSDYVLAHGLNTDDQLAF
                     GIVYQF"
     gene            1617..2468
                     /gene="hchA"
                     /locus_tag="OHN27_002872"
     CDS             1617..2468
                     /gene="hchA"
                     /locus_tag="OHN27_002872"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCQ2287274.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADIVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(2576..3934)
                     /gene="hprS"
                     /locus_tag="OHN27_002873"
     CDS             complement(2576..3934)
                     /gene="hprS"
                     /locus_tag="OHN27_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCQ2287275.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(3934..4605)
                     /gene="hprR"
                     /locus_tag="OHN27_002874"
     CDS             complement(3934..4605)
                     /gene="hprR"
                     /locus_tag="OHN27_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCQ2287276.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHTLNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4738..5151
                     /gene="hiuH"
                     /locus_tag="OHN27_002875"
     CDS             4738..5151
                     /gene="hiuH"
                     /locus_tag="OHN27_002875"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCQ2287277.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5259..6263
                     /gene="msrP"
                     /locus_tag="OHN27_002876"
     CDS             5259..6263
                     /gene="msrP"
                     /locus_tag="OHN27_002876"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCQ2287278.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6264..6899
                     /gene="msrQ"
                     /locus_tag="OHN27_002877"
     CDS             6264..6899
                     /gene="msrQ"
                     /locus_tag="OHN27_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCQ2287279.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSFFNRLRKQVHNKLSV"
     gene            7156..7806
                     /gene="zinT"
                     /locus_tag="OHN27_002878"
     CDS             7156..7806
                     /gene="zinT"
                     /locus_tag="OHN27_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCQ2287280.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            8149..8679
                     /locus_tag="OHN27_002879"
     CDS             8149..8679
                     /locus_tag="OHN27_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCQ2287281.1"
                     /translation="MNRFSKTQIYLHWLTLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(9249..9338)
                     /locus_tag="OHN27_002880"
     tRNA            complement(9249..9338)
                     /locus_tag="OHN27_002880"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9302..9304),aa:Ser,seq:cga)
     gene            9432..10229
                     /gene="mtfA"
                     /locus_tag="OHN27_002881"
     CDS             9432..10229
                     /gene="mtfA"
                     /locus_tag="OHN27_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCQ2287282.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            10330..10405
                     /locus_tag="OHN27_002882"
     tRNA            10330..10405
                     /locus_tag="OHN27_002882"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10363..10365,aa:Asn,seq:gtt)
     gene            10718..18700
                     /locus_tag="OHN27_002883"
     CDS             10718..18700
                     /locus_tag="OHN27_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HCQ2287283.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSKKNLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQTSGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDSDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPSSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSTQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATIDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPPGEITVTDTAPQQLTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNGGKGMTDSNGVAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVYTAEATLPNGNRDTKIVNIAPDASNALVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPLAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTLKGTVLGVHT
                     VEATLLNGNGYTTTVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNEVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLRVSPSEGVTLSNNGINTTNHDGYLYASF
                     TATKAGVYQVTATLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTAS
                     ITGGKSEQLVVNFTADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTTVKVTGNPSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSTTLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASKS
                     VLKNNRSSLKGDFTDSAELYLVLHDISGNPIKVSEGLEFVQSGTNVPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSNWER
                     ITATPKSGGPSYVYEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPRADHLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFQSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(18962..20014)
                     /locus_tag="OHN27_002884"
     CDS             complement(18962..20014)
                     /locus_tag="OHN27_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCQ2287284.1"
                     /translation="MFLASLLRRIAFSYYAYKAYNFNIEKTEFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSIINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     SLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIANIQSETDMPIVIVHGPKGEDKARELVNSYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGKKFDNI
                     SLDEFAKALKSVLARI"
     gene            20329..21645
                     /gene="shiA"
                     /locus_tag="OHN27_002885"
     CDS             20329..21645
                     /gene="shiA"
                     /locus_tag="OHN27_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCQ2287285.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPENKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            21747..>23184
                     /gene="amn"
                     /locus_tag="OHN27_002886"
     CDS             21747..>23184
                     /gene="amn"
                     /locus_tag="OHN27_002886"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCQ2287286.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDQLHSRKLRTFN"
BASE COUNT         6373 a         5503 c         5260 g         6048 t
ORIGIN      
        1 cgttattagt tgctggcgca gcaaatgcgg ctgaaattta taataaagat ggcaataaac
       61 tggatctgta cggcaagatg gttggtgagc gcattctgac cgaccgtgaa aatggtgaaa
      121 aaggcgataa ttctcaagat acctcttatg cccgtgtagg cgtgaaaggt gaaactcaga
      181 tcaaccctga attgactggt tacggtcagt ttgaactaga cctggaagca tcaaacagac
      241 acaaccctga ccaaactcgt ttggcttatg ctggtttgag ttacaaagat tttggttcat
      301 tcgactatgg tcgtaatgtc ggtgtcgcgt atgatgcaga agcatttact gatatgtttg
      361 ttgagtgggg cggtgattca tgggctggta ctgacctgtt catgaccaac cgtaccaacg
      421 gtgttgcaac ctatcgtaac acagatttct ttggcatggt tgaaggtcta aattttgctc
      481 tgcaatatca gggtaaaaat gaaggtacag gcaactacaa agcaaatggc gatggccatg
      541 gtttatctgt aacttacact attgacggtt tctcatttac tggtgcttac gcaaactctg
      601 accgtactga tttccagtct agtgatagta aaggtgaaaa agcagaagtt tgggcactgt
      661 caacaaaata tgatcccaac aacgtctatg ctgctgtgat gtacggtgaa tctcacaaca
      721 tgaattccga cgacggtgat gttgtgaaca aaacacaaaa cttcgaagct gttctgcaat
      781 atcagttcga cttcggtctg cgtccatcca tcggttactc ctattctaaa gcactggacg
      841 tagcaggtta caaagattct gatcgtctga actacattga aatcggtacc tggtattact
      901 tcaataagaa catgaacgta tataccgctt atcagatcaa cctgcttgat aagagtgatt
      961 atgttctcgc tcatggactg aacaccgacg accaactcgc atttggtatc gtctaccagt
     1021 tctaatcagc caacgcgccg cggcgtgtta ctgtaaaaca taacactagc ctgagttcgt
     1081 ccctcatcga actcaggctt ttttattggc atttcatctc catctgcggc acacgcaatt
     1141 gccaaagcat atcgatacag caccttaccc tgcccctcct ggcacaatat ctctaccagc
     1201 tactcttcca gccaacactc caaatacatt tctttatgta ttaatccgca acaaacacgc
     1261 ctcccatata gaaatatttg caaaaatagt gcatgaaata agctttttct ctaccaatcc
     1321 gggatataaa tattcatcag caccacaata ttccacagaa ctaaatctct tccctccgcc
     1381 ccgcatctct gataatggtc taaaatcatt gaggccactt gcgacgacgt tgctcaataa
     1441 ggaaaatttg gcgtgaataa tattcaacac cagtgcagca tcatcattac atacttacac
     1501 ccgcagacta ccgctttcga tatattgact caattttcca gactggttaa aaatagcgac
     1561 cagctcagtc gcaaatatag tgactaccct aactaagcaa caataaggaa tacactatga
     1621 ctgttcaaac aagtaaaaat ccgcaggtcg atattgctga agataatgca ttcttccctt
     1681 cagaatattc gcttagccaa tataccagtc ctgtctctga tcttgatggc gtggactatc
     1741 caaaaccgta tcgcggtaaa cataaaattc tggtgatcgc cgcggacgaa cgttatttgc
     1801 cgaccgataa cggaaaactg ttctcgaccg gtaaccatcc gattgaaacg ttgctgccgt
     1861 tgtatcatct ccatgctgca ggtttcgaat tcgaagtggc gaccatttcc ggtctgatga
     1921 ccaagtttga atactgggct atgccgcaca aagatgaaaa agtaatgcca ttctttgagc
     1981 agcataaatc gttgttccgc aatccgaaaa aactcgcgga tattgtcgcc agcctcaacg
     2041 cagacagcga atatgcagca atctttgttc ctggtggtca tggcgcactt attggtttac
     2101 cagaaagcca ggacgtggct gctgctttgc agtgggcaat caaaaatgac cgttttgtta
     2161 tctccctttg ccacggcccg gcggcttttc tggcgcttcg ccacggcgat aacccactga
     2221 atggttattc catttgcgca ttcccagacg ccgcagacaa acaaacgcca gagataggct
     2281 atatgccggg tcatctcacc tggtacttcg gcgaagagct gaagaaaatg ggcatgaaca
     2341 ttattaatga cgacatcacc gggcgagtac ataaggaccg taaagttctc accggcgaca
     2401 gtcctttcgc agcgaatgcg ttgggtaaac tggcggcgca ggaaatgctg gcagcttatg
     2461 cgggttaatc cctgttacgt atgaatgtaa gcgcataatc aatcgcagcc acgttaatac
     2521 tcacgacgtg gctgcgtttt ttcatggttg atttatggcg tactgaaggc ctatgttaat
     2581 ttctttgcgg taacgtaatt cggaacacat tatgcttatt aaaatagtga tacgtagcac
     2641 ttcccccatg caattcggca atcgctttga ctaaagaaag accaagtccc tgaccttcgg
     2701 aatgacgcga attatctccc cgccaaaatc tacggaagag tttttcaggc tcattaattt
     2761 tcgttccagg actggcgata tcaatattaa gatagccgtt ggtatcaaga aaactggtta
     2821 tatgaatgcg cgatttttct ggtgaatatc taatggcatt aacaataaga ttcgataaca
     2881 ttcgttgcag taaaatttta tccgcaaaga tttgctgatt gcactcgacc ttaaagcaaa
     2941 tctctttctc gtctgaaagg tattcaagat aatccaacaa attttcgact tccttattga
     3001 gaaaaagtga gtccagtttc accaaaacat tgtttttatc tgcccgggca agaaacagta
     3061 tgttctctgt taaccgcgaa atattttcca gctcttcaat gttaccggca attgtttttt
     3121 gatattcagc gatacttcta gtttgactga gcgtaacctg attctgaccc agtaatgcat
     3181 taatgggcgt tctaagttca tgagcgagat cgtcagcaaa ctgacttaga cgctcaaaat
     3241 ctttgactaa agcatgatgc attttattca acgcctgccc aagaggtttt agttctctcg
     3301 gtaatgcgct aacctcaaca ggctcccggc tatcgttata attcagcgct tccgttacac
     3361 cactcaactt tttgatctct cgtaatcccg ttctgattaa cagcggactt aatactgagc
     3421 aaagtacaat ggcgacaatg caaattataa tgctattaat tttatactgt tcaagcatgt
     3481 tatgtctggc tgaagccaat ttagccacag taaccgttaa tgatggcgaa acttcatcaa
     3541 tattaattcg taaagcatct atctctgtat cattaataat gcttctgtaa atgccagctg
     3601 cgctgattgt ctcagtagca ggtatgttat ttaacatgtc atcactgaca ttcgtccggt
     3661 taacaatttt gttgatgcta tcaccatgaa tgatcaagat atcctgacta acatccatca
     3721 tccggttaaa gtacacaggt aacgtatctg gatttacccc atcaattaac aactgcttga
     3781 tctgcgctgt ccggttaatg agtgttgtat catcgcgcca tttcaactca cttgccaggc
     3841 cattatagag agtccataca attccagcgc cagcaacaga cagcagcaat ataaaaagca
     3901 aggttaaacg gacggttata gataatcttt tcattatttt tttaccgcta cgaatgaata
     3961 gcccatcccc cggattgtgg cgattagctt ttcaggaaaa ggatcatcaa cttttgcgcg
     4021 gagcctgcga atggcgacgt ccaccgtatt ggtatcacta tcaaagttga ttccccaaat
     4081 ttcactcgca ataaccgttc tggggataat ctcgccagct ctggaagcca gtagccaaag
     4141 taactgaaac tccttgcgcg tcagtgtaat actgatattg tccctgctca cactttgact
     4201 aacagagtcc attcttaagc cactgatttc taatgttgaa ttcagagtgt gatgttgcct
     4261 taattgtgcc cgaacccttg ccagcaactc agaaaatgaa aaaggtttta ccagataatc
     4321 atttgcccca ctgtccagcc ctctgactct gtcatcgaca gaatctcttg cagtaaggca
     4381 aataacagga gtttgctttg ctgttcttaa cgtttgtaag atctgccagc catccatacc
     4441 cggaagcata atatccagaa tgatcaatgc ataatcatcc ttaagcgcaa gataaagccc
     4501 atctctgcca tcagaaacgg catcaatgac ataacccgct tcggaaagcc cctgcgttac
     4561 ccactcctgg gtcctttgat tatcttcaat aagtaaaatc ttcatgaaaa taatatgcca
     4621 taaatcatat gttatagaaa cagcctggtt cattacaaaa ttgtaatgct gctgtaaggt
     4681 taccgtggac gctttttcgc tatcctcaaa actcattcac atgacaagga tataaacatg
     4741 ttaaagcgtt atttagtact ctccgtagta acggctgcat tttcattacc ttctttggtc
     4801 tatgctgcac aacaaaacat tcttagtgtg cacattttga accagcaaac aggaaaaccc
     4861 gctgccgacg tgacagtcac tcttgaaaag aaagcggaca acggctggtt acaacttaat
     4921 accgccaaaa cagataagga tggacgaatt aaggcactgt ggcccgagca aactgcaact
     4981 acgggcgatt accgtgtcgt atttaaaacc ggggactatt tcaagaaaca aaatcttgaa
     5041 agtttcttcc ctgagatccc cgttgaattt catattaata aagtgaacga gcattatcat
     5101 gtgcctttat tacttagcca atatgggtat tcaacctatc gtggcagtta atgcataaat
     5161 atgtaaaagc agatctctgc tactgttagg gaggacactc cctgacagat taacagtaaa
     5221 cggctcttgc tggctaacga caaaaagtgt gatggcttat gaaaaagaat caatttttaa
     5281 aagaatcaga tgttacggcc gagtcggtat tctttatgaa gcgtcggcag gtgttaaaag
     5341 cactgggcat cagcgcagct gcactttctt tgcctcacgc tgcgcatgcc gatctgctta
     5401 gctggtttaa agggaacgat cgcccacccg cccccgccgg aaaagcgctg gagttcagca
     5461 agcctgccgc ctggcaaaat aacctgccac tgacgccagc agataaagtc tccggttata
     5521 acaacttcta tgaattcggg ctggataaag ccgatcccgc cgctaatgct ggtagcctga
     5581 aaaccgatcc atggacactg aaaatcagcg gcgaagtggc aaaaccattg accctcgatc
     5641 acgatgattt aacccgtcgc ttcccgctgg aagagcgtat ttatcgtatg cgctgcgtgg
     5701 aagcgtggtc gatggtggtg ccgtggattg gttttccgct gcacaaattg ctggcgcttg
     5761 ccgaacccac cagcaatgcg aagtatgtcg ctttcgaaac aatttatgca ccggaacaga
     5821 tgccaggcca gcaggaccgc tttatcggcg gcgggctgaa atatccttat gtcgaaggat
     5881 tgcgtctcga cgaagcaatg catccgctca cactgatgac cgtaggtgtt tatggcaagg
     5941 cgttaccgcc acaaaatggc gcgccggtgc gactgattgt gccgtggaaa tatggcttta
     6001 aagggattaa atcgatcgtc agtattaagc tgacccgcga gcgtccgcca accacctgga
     6061 atctggcagc gcctgacgaa tacggttttt acgccaacgt taatccgcat gttgatcacc
     6121 cgcgctggtc acaggctacc gaacgattta ttggttcagg cggcatcctc gatgtacagc
     6181 gccagccaac gctactgttt aatggttacg ccgaccaggt ggcatcgctg tatcgtggcc
     6241 tggatttgcg ggagaatttc tgaatgcgtc tgacagcaaa acaggtgaca tggctgaaag
     6301 tttgcctgca tcttgccgga ttgttgccgt ttctctggct ggtctgggcg atcaatcacg
     6361 gtggactggg tgccgatcca gtgaaagata ttcagcattt taccggtcgc actgcactga
     6421 aatttttgct ggcgaccttg ttaatcacac ctctggcacg ctacgcaaaa cagccgttat
     6481 tgatacgcac tcgccgcctg ttaggcttat ggtgctttgc ctgggcgaca ctgcatttaa
     6541 ccagttacgc attgctggag ctgggcgtga ataatctggc gttattaggt aaggagttaa
     6601 ttacccggcc ttatttaacg ttaggtatta tcagctgggt tattttgctt gctttagcgt
     6661 tcacttcaac ccaggcgatg cagcgaaaac tcggcaaaca ctggcaacag ttgcataact
     6721 tcgtctatct tgtcgcgatc ctggccccaa tacattatct atggtcagtg aagattattt
     6781 caccgcagcc actcatctac gctgggctgg ctgtactgct tttagcctta cggtataaga
     6841 agttgctttc ctttttcaac cggttacgca agcaggttca caacaaatta tctgtataac
     6901 ttcatattgc cgacaaagta cgcgttatcc ctgccggatg cggcgcgagc gccctatccg
     6961 gcatacacag atcgcattag gttatgtttc gtttgtcatc aacctcaaat taccaacata
     7021 atttatttta tttcaataag atagataagt agaactgaga aagccatgct ctcgtttcct
     7081 aagagttgtt gcattttgtt atatgttaca atataacatt acacatcata tacattaact
     7141 ctggaggaaa ctgttttggc gattcgtctt cataaactgg ctgttgcttt aggtgtcttt
     7201 attgttagcg ctcctgcctt ttcgcatggt catcactcac acggcaaacc cttaacagag
     7261 gtcgaacaaa aagctgctaa tggtgttttt gatgatgcca atgtacaaaa ccgaacgctc
     7321 agtgactggg atggagtctg gcaatccgtt tatcctttac tgcaaagtgg caaacttgac
     7381 cccgtctttc agaagaaagc ggatgcagat aaaactaaaa catttgctga aattaaagat
     7441 tattatcaca aaggttatgc aacagatatc gagatgattg gcattgagga cggcattgtt
     7501 gaattccata gaaataatga aacaacatcg tgtaaatatg attacgatgg atacaaaata
     7561 ctcacctata aatcaggcaa gaaaggcgtt cgttacttat tcgaatgtaa agatcctgaa
     7621 agcaaagccc ctaaatatat acaatttagc gatcatataa ttgcaccacg aaaatcatct
     7681 cattttcata tttttatggg taatgattca cagcaatctt tattaaatga aatggaaaac
     7741 tggccaacgt attatccata tcagttgagt agcgaagaag tggtcgagga aatgatgtct
     7801 cattgagtat tctcatgata acgcctcgat gccgctttag taagtcatca taactgccac
     7861 tggtccatcc acaaacgcca ctgaatgcaa gctagctaca gacacgctca tcactatgac
     7921 gtgtctgtat attaataagc taacccgcat tgagttaacc aataacggat tccatacaca
     7981 atacggccaa cagagaaaga tacctgtgct cacgccattg cttatattgg ctggttacaa
     8041 tgtgcactat caatttttta aataacaaat tcttaaaacc acatctgtat aaggaatttt
     8101 taaggttcgt gggatagctt gactgtgaaa atcacaggag cttcaaaaat gaaccgattc
     8161 tcaaaaactc aaatttattt acattggtta acgctgcttt tcgttgcaat aacctatgcc
     8221 gcgatggaac tccgtggctg gtttcctaaa ggtagtagta cttatctgct gatgcgagaa
     8281 acacattaca atgcgggtat attcgtttgg gtgttaatgt tttcacgcct gattataaaa
     8341 caccgttata gtgatccttc tattgtgcca ccgccacctg cctggcaaat gaaagcggct
     8401 tcgctaatgc acatcatgct ttatataacc ttccttgcac ttcctctgct ggggattgct
     8461 ttgatggctt acagtggaaa atcgtggagt ttccttggtt tcaatgtgtc tccctttgtt
     8521 accccaaaca gcgaaattaa agcactgata aaaaatattc acgaaacctg ggcaaatata
     8581 ggctactttt taatcgcagc tcacgctggc gcagcactct ttcatcacta cattcagaaa
     8641 gataatactc tgttacgaat gatgcctcgc cgcaaataag tcgatcccct gacaaggaaa
     8701 tgtgcactat ggacatcaac gcgtcataaa tgtttacgca agtgcacaat gcaccctttc
     8761 cacgatcaac aaggaggagc agaaactgtg taaatcgtac aatggtaaga cttatcaaaa
     8821 atacaggtta attttggtat ttaaaccttt ttttcttgtt agtatgatac aaaggctttc
     8881 aaaaaagctg ggaaattcaa caactcttgg aaattatgga ctttgtcccg cggctttaat
     8941 ctgacggccg cgttcctttt ttgactcaat tattgacccc ttctctacgc aacttcagtt
     9001 cccaccacca actttgcggc agtttcatag gatgaatgtc taaaagaata atggtgagcg
     9061 ataagaaaac gactgaataa ctgcacattt tcgctcgaaa gcttcctgtc agatccatag
     9121 cgaatcaagt gctgaatgtc acagtatcga acagaaaaca gtgacgatct aacccttcaa
     9181 gaatattcta cgattgttct gtttaggaaa agcaaggcgg gaagtcggga gataagtcat
     9241 tgataaagtg gcggagagag ggggatttga acccccggta gagttgcccc tactccggtt
     9301 ttcgagaccg gtccgttcag ccgctccggc atctctccgt tcagatggtt gccatgatgc
     9361 caggaaattt ggcattttaa cagtccctgt ccgtgcaatt ttgttcaagt gacgagtttg
     9421 cgagcaaaac gatgattaag tggccctgga aagtacaaga atcagcacat caaactgccc
     9481 ttccctggca ggaagcacta tcgatccccc ttttaacgtg tctgacggaa caggaacaaa
     9541 gcaaattagt cactcttgcc gaacgttttt tacagcaaaa gcggcttgtt cctttacagg
     9601 gctttgaact ggattcatta agaagctgcc ggatagcact gctattttgc ctacccgttc
     9661 tggagttagg actggaatgg ctggatggtt ttcatgaagt cttaatttat cctgcgccat
     9721 ttgtggtcga tgatgaatgg gaagacgata tcggtctggt gcataaccaa cgtattgttc
     9781 agtcaggtca gagctggcag caagggccta tcgttttgaa ctggttggat atacaagatt
     9841 cttttgatgc ttctggtttt aacctgatta ttcatgaagt cgctcataag ctggacaccc
     9901 gtaacggcga tcgcgccagc ggagttccct ttattccgtt gcgtgaggtt gctggctggg
     9961 aacacgatct tcatgctgca atgaacaaca ttcaggaaga aatcgaattg gttggcgaga
    10021 atgcggcgag cattgatgct tatgctgcca gtgatcctgc tgaatgtttt gccgtacttt
    10081 ctgaatattt ctttagcgcc ccagaacttt ttgctcctcg tttcccttca ttgtggcaac
    10141 gtttctgcca attttatcaa caagatcctt tgcagagact gcatcacgct aatgatacag
    10201 actcgttttc ggcgacgaat gttcattaat taacaacttt gcagattaat taaccaattg
    10261 aaatgactta tgaaatttag tgttgacaga caaggtaccg ctaagtaata tgcgccccgc
    10321 tcacacgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact
    10381 ggttcgagtc cagtcagagg agccaaattc taaaaattcg cttcttagtg caatgtcact
    10441 aaccttagtt gaacattgtt ttttaacgga tagcggtttt ttaacatctt aagcggcctc
    10501 gacctttatg gttgagggcg ttttgctatg aatgccatca ccattttccc ctcgattata
    10561 aaacttgagt tattcagtag tctcccctct tgcaactcac acccaaaact gcctaacgaa
    10621 aagttattaa ttttcaatca tattgctatc aggatttaca ttttttcgct gtactagaaa
    10681 gggcgcattt atgttagctc attcagggaa ggtaagcatg gctacgaaga agagaagtgg
    10741 agaagaaata aatgaccgac aaatcttatg cgggatggga attaaactac gccgcttaac
    10801 tgcgggtatc tgcctgataa ctcaacttgc gttccctatg gctgcggcag cacaaggtgt
    10861 ggtaaacgcc gcaacccaac aacccgttcc tgcacaaatt gccattgcaa atgccaatac
    10921 ggtgccctac acccttggag cgctggaatc ggcccaaagc gttgccgaac gtttcggtat
    10981 ttcggtggct gagttacgca aactcaacca gtttcgtacg tttgctcgag gttttgataa
    11041 tgtccgccag ggtgatgaac tggatgtccc ggcacaagtt agtaaaaaaa atttaacccc
    11101 gccgccgggt aatagcagtg acaacctcga gcaacagata gccagtactt cacagcaaat
    11161 cgggtctctg ctcgccgaag atatgaacag cgagcaagca gcaaatatgg cgcgtggatg
    11221 ggcctcttct cagacttcag gcgcaatgac agactggtta agccgcttcg gtaccgcaag
    11281 aatcacgctg ggcgtggatg aagattttag cctgaagaac tcccagttcg attttctcca
    11341 tccatggtat gaaacgcctg ataatctctt tttcagtcag catactctcc accgtactga
    11401 cgagcgtacg cagattaaca acggcttggg ttggcgtcat ttcactccca catggatgtc
    11461 gggcatcaac ttctttttcg accacgacct tagccgttat cactcccgcg ccggcattgg
    11521 cgcggagtac tggcgcgact atcttaaatt aagcagtaac ggctatttac gactgaccaa
    11581 ctggcgcagc gcacctgaac tggacagcga ttatgaagca cgcccggcca atggctggga
    11641 tgtacgcgca gaaggctggt tacccgcctg gccgcacctt ggcggtaaac tggtctatga
    11701 acagtattat ggcgatgaag tggccctgtt cgataaagat gatcggcaaa gtaatcctca
    11761 tgccataacc gctggactta actatacccc cttcccgctg atgaccttta gcgcggagca
    11821 acgtcagggt aaacagggcg aaaatgacac ccgttttgcc gtcgatttta cctggcaacc
    11881 tagcagcgca atgcagaaac agcttgaccc gaatgaagtc gctgcacgac gtagccttgc
    11941 aggcagccgt tatgatctgg tggatcgcaa caacaacatc gttctggaat atcgcaaaaa
    12001 agaactggtc cgcctgaccc tgacagaccc cgtgacaggg aagtcaggag aagtgaaatc
    12061 actggtttcg tcgctacaaa ccaaatatgc cctgaaaggc tataacgtcg aagccaccgc
    12121 tctggaagct gccggtggta aagtggttac aacgggtaaa gatattctgg ttaccctgcc
    12181 ggcgtaccgg ttcaccagta cgccagaaac cgataacacc tggccgattg aagtcaccgc
    12241 tgaagatgtc aaaggcaatt tttcgaatcg tgaacagagc atggtagtcg ttcaggcacc
    12301 tacgctaagc cagaaagatt cctcggtatc gttaagtacc cagacgttga gcgcggattc
    12361 ccattcaacc gccacactga cttttattgc gcatgatgca gcaggtaatc ctgttatcgg
    12421 gctggtgctc tcgacgcgtc acgaaggtgt tcaggacatc accctttctg actggaaaga
    12481 taatggtgac ggaagctata cccagatcct gaccacagga gcgatgtctg gcacgctgac
    12541 gctgatgcca cagctgaacg gtgtggatgc ggctaaagcc cccgccgtgg tgaatatcat
    12601 ttctgtttcg tcatcccgga ctcactcgtc aattaagatt gataaggacc gttatctctc
    12661 cgggaatcct atcgaggtga cggtagaact gagagatgaa aatgacaaac ctgttaagga
    12721 gcaaaaacag caactgaata ccgcagtcag catcgacaac gtgaaacctg gtgtcactac
    12781 agactggaaa gaaaccgcag atggcgtcta taaggcaacc tataccgcct ataccaaagg
    12841 cagtgggctt actgcgaagc tgttaatgca aaactggaat gaagatttgc ataccgctgg
    12901 atttatcatc gacgccaacc cgcagtcagc gaaaattgcg acattatctg ccagcaataa
    12961 tggtgtgctc gccaatgaga atgcagcaaa caccgtctcg gtcaatgtcg ctgatgaagg
    13021 aagcaaccca atcaatgatc ataccgtcac gtttgcggta ttaagcggat cggcaacttc
    13081 ctttaacaat caaaacaccg caaaaacgga tgttaatggt ctggcgacta ttgatctgaa
    13141 aagtagtaag caggaagaca acacggttga agtcaccctt gaaaatggcg tgaaacaaac
    13201 gttaatcgtc agttttgtcg gcgactcgag taccgcacag gttgatctgc agaagtcgaa
    13261 aaatgaagtg gtcgctgacg gcaatgacag cgccacaatg accgcgacag ttcgggatgc
    13321 aaaaggcaac ctgctcaatg acgtcaaggt caccttcaat gttaattcag cagcagcgaa
    13381 actgagccaa accgaagtga atagccacga cgggatcgcc acagctacgc tgaccagttt
    13441 gaaaaatggt gattatacgg ttacggcctc tgtgagctct ggttctcagg ctaatcaaca
    13501 ggtgattttt atcggtgatc aaagtactgc agccctgacc ctcagtgtgc ccccaggcga
    13561 gatcaccgtc accgacacag cgcctcaaca gctgacggca accttgcagg ataaaaatgg
    13621 caacccacta aaagataaag aaatcacttt ctctgtgcca aacgacgtcg caagtcggtt
    13681 ctcgattagc aacggcggaa aaggcatgac ggatagtaac ggggttgcaa tcgcctccct
    13741 gaccggcacg ttagcgggca cgcatatgat cacggctcgt ctggctaaca gcaatgtcag
    13801 cgatacacag ccaatgacgt ttgtggcgga taaagacaga gcggttgtcg ttctgcaaac
    13861 atcgaaagcg gaaatcattg ggaatggcgt ggatgagaca actctgacag caacagtgaa
    13921 agatcctttt gataacgtgg ttaaaaatct gtcagtagtc ttccgcacct cccccgcaga
    13981 cacgcaactg agtctgaacg cgcgtaatac taatgagaac ggtattgctg aagttaccct
    14041 taagggcacg gttttggggg tttacacagc cgaagccaca ctgcctaacg gcaacagaga
    14101 tacgaagata gtcaatattg cgcccgatgc cagcaacgcg ctggtcacgc tgaacatccc
    14161 ggctcaacag gtggtgacga ataacagcga cagcgtgcag ctgacggcga cggtgaaaga
    14221 tccgtcgaat catccgctgg cggggataac ggtaaacttc accatgccac aggacgtcgc
    14281 ggcaaacttt acccttgaaa ataacggtat tgccatcact caggccaatg gcgaagcgca
    14341 tgtcacgctc aaaggtaaaa aagcgggcac acatacggta accgcaacgc tgggtaacaa
    14401 taatgccagc gatgcgcaac cagtcacctt cgtggcggat aaggacagcg cagttgtcgt
    14461 tctgcaaaca tcgaaagcag aaatcattgg gaatggcgtg gatgagacga ctctgacggc
    14521 aacagtgaaa gatccttttg ataacgcagt aaaagatcta caggtcacct tcagtaccaa
    14581 cccggcagat actcaactta gtcagagcaa aagcaatact aacgacagtg gtgtggctga
    14641 agttaccctt aagggcacgg ttttgggtgt tcatacagtc gaagccacac tgcttaacgg
    14701 caatggctat acgacgacag tcaatattgc gcccgatgcc agcaacgcgc aggtcaccct
    14761 gaacatccct gctcaacagg tggtgacgaa taacagtgac agcgtgcagc ttacggcgat
    14821 ggtgaaagat ccgtcgaatc atccggtggc ggggataacg gtaaacttca ccatgccaca
    14881 ggacgtcgcg gcaaacttta cccttgaaaa taacggtatt gccatcactc aggccaatgg
    14941 cgaagcgcat gtcacgctga aaggtaaaaa agcgggcacg catacggtta ccgcaacgct
    15001 gggtaataac aataccagtg attcgcagcc ggtaacattt gtggcggaca aaacctcggc
    15061 tcaggttgtc ctgcagatgt caaaagatga gatcacaggt aatggcgtcg ataacgcaac
    15121 gctaactgca acggttaaag atcagttcga caatgaggtg aataatcttc cggtaacatt
    15181 cagctcagcc tcttcaggac tcaccctgac cccgggagta agtaatacca atgagtctgg
    15241 catcgcgcag gccactctcg caggcgttgc ctttggtgag cagacggtca ctgcatcact
    15301 ggctaataat ggtgccagcg acaacaaaac tgtgcatttt attggcgaca cagcggcggc
    15361 aaaaattatc gagttgacgc ctgtcccaga cagcataatc gccggtaccc cgcagaacag
    15421 ctccggcagc gtcatcaccg ccacagtcgt tgataataat ggctttccgg tgaaaggtgt
    15481 gactgtgaac ttcaccagca acgcagcgac agccgaaatg acgaacggcg gtcaagccgt
    15541 gacgaacgaa cagggtaagg ctaccgtcac ttataccaat acccgctcct cgatagaatc
    15601 aggagcgaga ccggataccg ttgaggccag tctggaaaat ggtagctcca cgcttagcac
    15661 atcaattaat gtcaacgctg atgcgtctac ggcacatctc accttgctac aggcactttt
    15721 tgatacagtc tccgcaggcg acactaccaa tctgtatatt gaggtgaagg ataattacgg
    15781 caacggagta ccccagcagg aggtaaccct cagagtatca ccaagtgaag gcgtgaccct
    15841 cagtaataac ggcataaata ctaccaacca cgacggttat ttatacgcaa gctttaccgc
    15901 tacaaaagcc ggggtttatc aagtgacggc aaccctcgaa aatggcgatt cgatgcaaca
    15961 aacagtgacc tatgtgccga acgtcgcgaa tgccgaaatc acgctggcag cctcgaagga
    16021 tccggtgatt gccgacaata acgatctcac gacactaaca gcaacagtcg ctgatacaga
    16081 gggcaatgcg atagccaaca ctgaagtaac atttactctg ccggaagatg tgaaggcgaa
    16141 cttcacgctg agcgatggcg gtaaagcgat tactgatgct gaaggcaaag cgaaagtcac
    16201 gctgaaaggt acaaaagcag gcgctcatac tgttacagca tcgataactg gcggtaagag
    16261 tgagcagttg gtggtgaact ttactgcgga tacgctcact gcgcaggtta atcttaacgt
    16321 taccgaggac aattttatcg ctaataacgt cgggatgacc aggctgcagg caacagtgac
    16381 tgatggaaac ggcaacccgt tagccaatga ggcggtgaca ttcacgctac cggcagatgt
    16441 gagcgcaagc tttactctcg gacaaggcgg ttccgccatt actgacatca acggcaaggc
    16501 tgaagttaca ctgagcggta caaaatccgg cacctacccc gtgacagtta gcgtgaacaa
    16561 ttatggtgtc agtgatacga aacaggtgac tttgattgcc gatgctggta ccgcaaaact
    16621 agcctcctta acctctgtat actcattcgt cgtcagcacg accgagggcg cgaccatgac
    16681 tgcaagcgtc actgacgcta acggcaaccc ggtagaaggt ataaaagtta atttccgcgg
    16741 aacttccgtc acgctaagca gcaccagcgt tgaaacggat gatcggggtt tcgctgaaat
    16801 tcttgtgaca agcaccgagg tcggactgaa aacagtttca gcctctctgg cagataaacc
    16861 tactgaagtc atctcgcgat tactgaatgc aaaagcagat attaattctg caacgattac
    16921 cagtctggag atacctgaag gtcaggtcat ggtcgcacaa gacgtagcag ttaaagctca
    16981 cgtcaacgac cagtttggca atccgattct taatgaatct gtaacattca gtgcagaacc
    17041 accagagcac atgaccatca gccaaaatat tgtctctact gatacgcatg gtatagccga
    17101 ggtcactatg acgcccgaaa gaaacggttc gtatatggtg aaagcatccc tggcgaatgg
    17161 atcctcttat gagaaggatc tggtggtaat cgatcaaaaa ctgacactct cggcgtccag
    17221 cccgcttatc ggtgtcaatt ccccaacagg tgcaactctg acggcaacgc taacttctgc
    17281 aaatggcact ccagtggagg gtcaggtcat caactttagc gtaacgccag aaggtgcgac
    17341 gttaagtggc ggaaaagtga gaaccaactc ttcaggtcag gctccagtcg ttctgaccag
    17401 caataaagtc ggtacatata cggtgactgc atcgttccat aacggcgtca caatacagac
    17461 acagacaacc gtgaaggtta ctggcaaccc aagcaccgca catgttgcta gctttatcgc
    17521 tgatccgtcg actatcgccg ccaccaacag tgatttaagt accttaaagg caacggttga
    17581 ggatggcagt ggtaacctga tcgaaggtct cactgtgtac ttcgccttaa aaagcggctc
    17641 caccacatta acgtcattaa cagcggtgac agatcaaaac ggaatcgcga caacaagcgt
    17701 gagaggagcg ataacgggga gcgtcacggt aagcgcagtc acgaccgctg gtggaatgca
    17761 aacagtagat ataacgctgg tggcaggccc ggcagacgcc tcaaagtcag tccttaagaa
    17821 caatcggtca tcattgaaag gagactttac cgatagtgct gagctatatc ttgttctgca
    17881 cgatatatca ggcaatccga tcaaagtttc tgaagggctg gaatttgtgc agtcaggtac
    17941 caacgtgccc tatgtgcaag ttagtgcaat tgactacagt aaaaatttct caggcgagta
    18001 caaagccact gttacaggcg gcggagaggg tatcgcaacg ctgatccctg tattgaatgg
    18061 tgttcatcaa gctggtctga gtaccacaat acaattcact cgcgcagaag acaaaataat
    18121 gagcggtaca gtgtcagtca atggtactga cctaccgaca actacattcc cttcgcaggg
    18181 attcaccggg gcgtattatc agttgaataa tgacaacttt gcccctggaa aaacggcggc
    18241 tgattatgag ttttcaagct ctgcctcctg ggtcgatgtt gatgctaccg gtaaagtgac
    18301 atttaaaaat gtcggcagca attgggaaag gattacggcg acgccaaaat caggaggccc
    18361 tagctatgta tacgaaatcc gtgtgaagag ttggtgggtg aacgccggcg atgctttcat
    18421 gatatacagc cttgctgaaa atttttgcag cagcaatggc tacacacttc cccgtgcaga
    18481 tcatttaaac catagtcgtt cccgaggcat cgggtcactg tacagtgaat ggggagatat
    18541 ggggcattac acgactgaag ctggttttca atcaaatatg tattggtcat ctagtcccgc
    18601 aaactcaaac gaacaatacg tagtttccct ggcaacaggt gatcaaagcg tatttgaaaa
    18661 gcttgggttt gcttatgcga catgttataa aaacctctga ttttccgtta taatttctta
    18721 aagagtgctc cattcggggc actctttttt acattccctc tatagagctt gctcttctct
    18781 caccgaaaat gaataaataa aaatgcgata ccgccagatt cgcgttcggt gtgactatga
    18841 tcgtatccgg tgagtacagc tttgatccaa aggctggttt tctgtgatga ttgaccagcc
    18901 tcagagtatt gatgacacca ccgttcataa cagatcggca ttactttgca tacgtactgc
    18961 ctcaaattct cgccaaaaca ctttttaatg cctttgcgaa ttcatccaga cttatattgt
    19021 caaatttttt cccaacgacg actgactccg aaacatcggc cattgcgggc catcgcgttt
    19081 tgtaatcagc ataaattgca acaacggggg tattataggc acttgccata tgtaatattg
    19141 aagtgtcagg agttattgcg atataagcat cttttatgat tgctgctgaa cgtttaatcg
    19201 atggtgacaa ggataaacgg tagacattat tataactatt cactagttcc ctggctttat
    19261 cttccccttt gggtccatga acgatcacaa ttggcatatc tgtttcactt tgaatgtttg
    19321 ctattagatt ttctgcaatc gataatgaaa atgtacgttc ctgcgagcta ccttctaaat
    19381 taagcgcaat gtaagatcct aacgatcgca tttcctcgcg cacctcatcg agtacatcct
    19441 cacttagtgg caattcatat attggtctga ctgctggaaa acccgcctca cgcatcataa
    19501 atgcccatgt catgggtaca ggagcccgga gactctgatc catccgggac gcgttcttac
    19561 acaaagggga atagcacttc atggttaaac caacaacctg aaaattcgtt tttgctttca
    19621 actgactgat aaataacatc gttttcaggt tctttttacg catcgcctct atacaaagat
    19681 ccggcgtacc gtattgctgt gttatgttct tcgctaaatc ttttatttct tttaatgttg
    19741 cgtgatcctg catagtcatt gtgactaatg ttaatttagt ctgttcaagt ttgagcgcat
    19801 taaagacttc taaattaatt atcgacgtaa caattagtag atgcttaatt ttatgcaatt
    19861 caagcgcccg aataactgga aagatggcca tagcatcgcc aatctgatcg ggaatatgga
    19921 taacaacaaa ctctgttttt tcaatattga aattataagc tttataagcg tagtaactaa
    19981 atgcaatacg tctcaacaat gatgctaaaa acatacctaa cctcgcctcc ctactggtta
    20041 taatgcaaag cagtctatca gactcatcag ggtgccattt tgtgcatatg cggactttta
    20101 tgtttcatat ctcgaacctg tgggtcctct gcttaatcct taaacaacac cagcaactcc
    20161 cgcgctttca tcttccagcg aatttttcat gttgccgcta atcagccata aaatcatttg
    20221 cagatgcgct ctgtcgaggt agtctcataa ggttcgttta tagatcgacg gcaatgtgag
    20281 ttaccttttc catactaatt ataaaaagac agtacaaaca ggatcattat ggactccacg
    20341 ctcatctcca cccgtcccga tgaagggacg ctttcgttaa gtcgcgcccg acgagctgcg
    20401 ttaggcagtt tcgctggtgc cgtcgtcgac tggtatgatt ttttactcta tggcattacc
    20461 gccgcactgg tatttaatcg cgagtttttc ccgcaagtaa gcccggcgat gggaacgctc
    20521 gccgcatttg ctacctttgg cgtcggattc cttttccgtc cgctcggcgg tgtcattttc
    20581 ggtcactttg gcgaccggct gggacgtaag cgcatgttaa tgctgaccgt ctggatgatg
    20641 ggcatcgcga cagccttgat tggtattctt ccttcattct cgaccattgg gtggtgggca
    20701 cctattttgc tggtgacact gcgtgccatt cagggatttg ccgtcggcgg cgaatgggga
    20761 ggcgcggcgt tgctttccgt tgaaagtgcg ccggaaaata aaaaagcctt ttacagtagc
    20821 ggtgtacaag ttggctacgg agtaggttta ctgctttcaa ccggactggt ttcattgatc
    20881 agtatgatga caactgacga acagttttta agctggggct ggcgcattcc tttcctgttt
    20941 agcatcgtac tggtactggg agcattgtgg gtgcgcaatg gcatggaaga gtccgcggaa
    21001 tttgaacaac agcaacatta tcaagctgcc gcgaaaaaac gcatcccggt tatcgaagct
    21061 ctgttacgac atcccggtgc tttcctgaag attattgcgc tacgactgtg cgaattgctg
    21121 acgatgtaca tcgttactgc ctttgcactt aattattcaa cccagaatat ggggctaccg
    21181 cgcgaacttt tccttaatat tggtttgctg gtaggtggat taagctgcct gacaattccc
    21241 tgttttgcct ggcttgccga tcgttttggt cgccgcaggg tttatatcac aggcgcgtta
    21301 atcggaacgt tgagcgcatt tcctttcttt atggcgcttg aagcacaatc tattttctgg
    21361 atagttttct tctccataat gctggcaaac attgcgcatg acatggtggt gtgtgtgcaa
    21421 caaccgatgt ttaccgaaat gtttggtgcc agttatcgct atagtggtgc tggagtcggt
    21481 tatcaggttg ccagtgtggt tggcggtgga tttacacctt ttattgccgc tgcgctcatc
    21541 acttactttg ccgggaactg gcatagcgtc gccatttatt tgctggctgg atgtctgatt
    21601 tccgcaatga ccgctttatt gatgaaagac aatcaacgct cgtgatagcc tggcgaagat
    21661 catccgatct tcgccttata cttttgtttc acattcctgt gacatactat cagatgtgcg
    21721 gtaattgtat ggaacaggag acacacatga ataataaggg ctccggtctg accccagctc
    21781 aggcactgga taaactcgac gcgctgtatg agcaatctgt agttgcttta cgcaacgcca
    21841 ttggcaaata tattacaagt ggcgaattac ctgatgaaaa cgcccgcaaa caaggtcttt
    21901 ttgtctatcc atcactgacc gtaacctggg acggtagcac aactaatccc cccaaaacgc
    21961 gcgcatttgg tcgttttact cacgcaggca gctacaccac cacgattact cgccctactc
    22021 tctttcgttc gtatctcaat gaacaactta cgttgctgta tcaggattat ggtgcgcata
    22081 tctctgtgca accctcgcag catgaaatac cttatcctta tgtcatcgat ggctctgaat
    22141 tgacacttga tcgctcaatg agcgctggtt taactcgcta cttcccgaca acagaactgg
    22201 cgcaaatcgg cgatgaaact gcagacggca tttatcatcc aacggaattc tccccgttat
    22261 cgcattttga tgctcgccgg gttgattttt ccctcgcacg gttgcgccat tataccggta
    22321 cgccagtaga acattttcaa ccgttcgtct tgtttaccaa ctacacacgt tatgtggatg
    22381 aattcgttcg ttggggatgc agccagatcc tcgatcctga tagtccctac attgcccttt
    22441 cttgtgctgg cgggaactgg atcactgccg aaaccgaagc gccagaagaa gccatttccg
    22501 accttgcatg gaaaaaacac cagatgccag catggcattt aattaccgct gatggtcagg
    22561 gtattactct ggtgaatatt ggcgtgggac cgtccaatgc taaaaccatc tgcgatcatc
    22621 tggcagtgct acgcccggat gtctggttga tgattggtca ctgtggcgga ttacgtgaaa
    22681 gccaggccat tggagattat gtccttgcac acgcttattt acgcgatgac cacgttcttg
    22741 atgcggttct gccgcccgat attcctattc cgagcattgc tgaagtgcaa cgtgcgcttt
    22801 atgacgctac caagttagtc agcggcaggc ctggtgagga agtcaaacag cggctgcgta
    22861 caggtactgt ggtaaccaca gatgacagga actgggagtt acgttactca gcttctgcac
    22921 ttcgttttaa cttaagccgg gccgtagcaa ttgatatgga aagtgcaacc attgccgcgc
    22981 aaggatatcg tttccgcgtg ccatacggga cactactgtg tgtttcagat aaaccgttac
    23041 atggtgagat taagcttccc ggccaggcta accgttttta tgaaggcgct atttccgaac
    23101 acctgcaaat tggcattcgg gcgatcgatt tgctgcgcgc agaaggcgac caactgcatt
    23161 cacgtaaatt acgaaccttt aatg
//
LOCUS       DAJOSY010000071        22575 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJOSY010000071 DAJOSY010000000
VERSION     DAJOSY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.071"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            198..1286
                     /gene="pdeL"
                     /locus_tag="OHN27_002887"
     CDS             198..1286
                     /gene="pdeL"
                     /locus_tag="OHN27_002887"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HCQ2287287.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1328..2260)
                     /locus_tag="OHN27_002888"
     CDS             complement(1328..2260)
                     /locus_tag="OHN27_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2287288.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(2352..2849)
                     /locus_tag="OHN27_002889"
     CDS             complement(2352..2849)
                     /locus_tag="OHN27_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCQ2287289.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3107..3712
                     /locus_tag="OHN27_002890"
     CDS             3107..3712
                     /locus_tag="OHN27_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCQ2287290.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            3752..4615
                     /locus_tag="OHN27_002891"
     CDS             3752..4615
                     /locus_tag="OHN27_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCQ2287291.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEGFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQIYGGNYVS"
     gene            4605..6152
                     /gene="fdrA"
                     /locus_tag="OHN27_002892"
     CDS             4605..6152
                     /gene="fdrA"
                     /locus_tag="OHN27_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCQ2287292.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLAEIEELFNTKAQSGSHEARYA
                     TIGSAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLDRGETPVDEQGLQFARGTKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIETI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6152..7570
                     /locus_tag="OHN27_002893"
     CDS             6152..7570
                     /locus_tag="OHN27_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCQ2287293.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7620..7916
                     /locus_tag="OHN27_002894"
     CDS             7620..7916
                     /locus_tag="OHN27_002894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287294.1"
                     /translation="MAQESVGLISVAHQAFVTIAGCGVNALSGLRMAQESVGLISVAH
                     QAFATTAGCGVDALSGLRVARESVGLISVAHQAFVTIAGCGVNALSGLRVAREL"
     gene            7992..8942
                     /locus_tag="OHN27_002895"
     CDS             7992..8942
                     /locus_tag="OHN27_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCQ2287295.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            8952..10334
                     /locus_tag="OHN27_002896"
     CDS             8952..10334
                     /locus_tag="OHN27_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCQ2287296.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKLHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMVVQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            10711..11760
                     /gene="yahK"
                     /locus_tag="OHN27_002897"
     CDS             10711..11760
                     /gene="yahK"
                     /locus_tag="OHN27_002897"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCQ2287297.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            12003..12818
                     /locus_tag="OHN27_002898"
     CDS             12003..12818
                     /locus_tag="OHN27_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287298.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFHILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            13231..13476
                     /locus_tag="OHN27_002899"
     CDS             13231..13476
                     /locus_tag="OHN27_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287299.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGWGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAVIRQIRLIT"
     gene            complement(13493..14164)
                     /locus_tag="OHN27_002900"
     CDS             complement(13493..14164)
                     /locus_tag="OHN27_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCQ2287300.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            14311..14586
                     /locus_tag="OHN27_002901"
     CDS             14311..14586
                     /locus_tag="OHN27_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2287301.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(14684..16270)
                     /gene="prpR"
                     /locus_tag="OHN27_002902"
     CDS             complement(14684..16270)
                     /gene="prpR"
                     /locus_tag="OHN27_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HCQ2287302.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESSKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            16509..17399
                     /gene="prpB"
                     /locus_tag="OHN27_002903"
     CDS             16509..17399
                     /gene="prpB"
                     /locus_tag="OHN27_002903"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HCQ2287303.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDNLFARSQVK"
     gene            17746..18915
                     /gene="prpC"
                     /locus_tag="OHN27_002904"
     CDS             17746..18915
                     /gene="prpC"
                     /locus_tag="OHN27_002904"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HCQ2287304.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAKHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            18949..20400
                     /gene="prpD"
                     /locus_tag="OHN27_002905"
     CDS             18949..20400
                     /gene="prpD"
                     /locus_tag="OHN27_002905"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HCQ2287305.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            20440..22326
                     /gene="prpE"
                     /locus_tag="OHN27_002906"
     CDS             20440..22326
                     /gene="prpE"
                     /locus_tag="OHN27_002906"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HCQ2287306.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         5630 a         5703 c         6070 g         5172 t
ORIGIN      
        1 gacttgtgat taccttttta aggtatttag cgtaactgtt tttgagcgag catcagaggt
       61 aaagataatc ttcttgatag tgatgtggga tgttatacgt atggcatcgc tgatgtttat
      121 ggttacccct tatgtgtgct caggaatcga caggtaatca ctcatactga acagcgataa
      181 aagataaagg tgtgttcatg aattcatgtg attttcgtgt ttttctgcaa gagttcggta
      241 caacggttca tttgtcattg cctggtagcg tatccgagaa agaacgactg ctactcaagc
      301 tgctgatgca gggaatgtct gtaacagaaa tatcacagta cagaaatcgc agtgcaaaga
      361 caatttcaca tcaaaagaaa cagctctttg agaaactggg gattcagagc gatattactt
      421 tctggcgcga tattttcttt cagtacaatc cggagatcat atccgccacg gggagtaata
      481 gtcacagata tattaatgat aatcactatc accatatcgt cacgcctgaa gccatcagtc
      541 tggcgttgga aaaccacgaa ttcaaaccgt ggatccaacc ggttttctgc gcgcagactg
      601 gggtactgac gggctgtgag gtgcttgtcc gctgggaaca tccacaaacg ggaattatcc
      661 caccggatca gtttattcct ctggcggagt catctggtct tatcgtcata atgacccgcc
      721 aactgatgaa acagactgcg gatattctga tgccggtaaa acatttgctg ccggataatt
      781 tccatattgg tatcaacgtc tcggcgggtt gttttttggc cgcgggattt gaaaaagaat
      841 gcctgaacct agtcaagaaa ttaggaaacg ataaaatcaa actggttctc gagctaacgg
      901 aacgtaaccc tattccggta acgccagaag ccagagcgat atttgacagc cttcatcagc
      961 acaacattac ctttgcgctg gatgactttg gtacgggtta tgcgacctat cgttacttgc
     1021 aggcgttccc ggtcgatttt attaagatcg ataagtcatt tgtgcaaatg gcgagtgtcg
     1081 acgaaatctc cggtcatatt gtggacaata ttgtcgaact ggcgcgtaag cctggtctga
     1141 gtatcgtagc ggaaggggta gaaacccagg agcaggcgga tttaatgatc ggtaaaggcg
     1201 ttcacttttt gcagggctat ttgtactccc cgccagttcc gggtaataaa tttatctctg
     1261 aatgggtaat gaaagcaggt ggttgatgta aaccgctatt cacagcgcat cgggaggttg
     1321 gcagcgatta attctcccga tgcagtaaat cctgatagat ccccgttaat atgccatttg
     1381 gtgcaaattg ctttttaatc cactgcgtca cctgacccgt agcagaatgc tgagtcgcca
     1441 gtaacatgcg cgaatcctgg cgcggattat ggatttgtcg ggtaaccagc agggattgcg
     1501 tcatcgcctc acggaccatg taatccggca aaaaaccaat tccttcacct aaaatctggc
     1561 actgacattt ggtgttgaaa tctggcacca gaattgactc ctgcccgtgc agcaaccagc
     1621 ccactttttt attaatcgta tgcgcggtgt cctccaccat aatattaggg tagagacgca
     1681 gttggctttc ggcgatgggt tccggcacaa aggctaacgg gtgatccggg gcgatagcaa
     1741 aagcccagcg aatcgcgcca atttcagtgt aatcaatacc gccgccgtcc agtagtgtat
     1801 cgggtgcgcc gatggcgata ttggcctgat tattaataat cgcatcccag acgccgttat
     1861 acacttcggt cgtcacggta atctggcagg taggaaactg ctttttcagc acctgtaata
     1921 agcgcgcggt gtgtttgggc gtataaagca gctgattaat acaaatgcgc acacgcgctt
     1981 ctatgccctg cgaaatagta tctatgctac gtttgatggc ataaaaatca ttcagcaggt
     2041 cggtagcttt gcggaaaaaa taacgcccgg actcggttaa ctcaatgctg cgggtactgc
     2101 gagtgaacag caccacatcc agccccgtct ccatacgctt aatggtgtag ctaatggcgg
     2161 aagtggttaa acccaactct tctgcggctt tactgaagct gccaaaacgc gcagcggtag
     2221 taaatgccaa cagattctct tcggtaaaaa ttgagttcat taaaccattc tcgccattat
     2281 ctactatgaa tttctccagc tatgactgta gaggtatcgg ttaaagatag ataatcattt
     2341 ttgaataact tttaataccc gtcgcgttta gtcgtcgctt cgcttaattt ttgtccacca
     2401 tattctgaaa ttatccccac gcagttaccc gccagaaaac ccagtaacgt caccgtccag
     2461 ttcatcccgc tggcaaaaaa cagcgtcatg ccgagaaagc caccgggaat aaatgacagt
     2521 aaccagaaac gcccttgcca gaccaccacc gccgagaagg gtacggtagt aattacgctc
     2581 gcccagaacg cggacagtcc acttacagag gccagccaac cagcagtcag cgcggcgaaa
     2641 aacgcccaca ccatcccgga ataattaacc gccagacttc tgataaaccc cgatttaccg
     2701 ccgcctgcgg cataaaaact gcaaaaggcg acgaagccaa tggttcctaa taattcccat
     2761 ccctgagaga gcccgacatg tgaagacact aactgccaaa gccctgcaca aataccgacg
     2821 gtgacacccg ttgccgtgag tccgttcatc ttgttcccct ctggttagac atggacgaaa
     2881 gctactctta catgtgcaga ctatagagaa ataaaacctg attgagtgaa agggtatcgg
     2941 gtcaaagaaa caaatattgc actaccgcac actgcgaaaa gattgttgaa catcattcaa
     3001 caaaaaggcg aaatacggcg aattaaattt aagcgggttc ttttatcaga ttatcgttgc
     3061 tattctcaat tcatcgaaaa cagccacaaa aaccagggga atgtgaatga gtataaaaaa
     3121 tctacctgcc gactatttat tggctgcaca acagggtgat atcgataaag taaaaacctg
     3181 ccttgcgctg ggtgtcgata ttaatacctg cgatcgtcag gggaaaacgg caattacgct
     3241 ggcaagttta tatcagcaat atgcttgcgt tcaggcatta attgacgccg gagcggatat
     3301 taataaacaa gatcatacct gtttaaatcc ttttttaatt agctgtctga acgatgattt
     3361 aacactacta cgaattattt taccggctaa acccgatctt aattgcgtaa cccgttttgg
     3421 cggtgtcggc ctgacgcctg cctgtgaaaa aggccattta agtattgtaa aagagctttt
     3481 ggcgcatacg gagattaacg ttaaccagac caaccatgta ggctggacgc cgctactgga
     3541 agcgattgtg cttaatgatg gtggtattaa acagcaggcg attgtgcagt tattgctgga
     3601 acacggtgcc agcccgcatc tgaccgataa atatggcaaa acgccactgg aactggcgcg
     3661 ggaacggggc tttgaagaga ttgcgcagtt actgattgcc gcaggtgcat aaaccgggag
     3721 gcttgctatc aacacaccag aaaaacggtg tgtgtgggcg ctaactgcgg atgcggattt
     3781 tctggcgcag cgggggcaag gacaggttga acaggtcttt gccagagcgg taaatatcgc
     3841 actcccggct cgccagcagt tgctgacgct gctttgtgaa gagtacgaca atgcgccaaa
     3901 cagttgtcgg ttggcactca ctcactttga tgatctgttc cggcatggtg ataaggttca
     3961 gtttgacgat caaggtatta cggttggtca acatcttcat atagagatga gtcgttgtcg
     4021 gcgttggctg tccccaacct tgcaaatgac cgctgtgaat tttcacctta tcgcctggca
     4081 acagtggcac gacattattc atcagcacct gggggaaaat gaaaccctgt ttaattatcg
     4141 cggcgataat ccgttttatc aggcgttaaa taaagaatta catattaaac gacgggcagt
     4201 tatacaggca gtaatcgata aacaaaatat cgcctcagcg gtcgccagta tgatgggctt
     4261 agggattggc cttacgccat cagccgatga ttatttaaca ggtctggcac ttattttatt
     4321 tatttccggg catccggcgg gaaaatacaa agagggattt tatctcggct tgcaacgcgg
     4381 cagaaataat accacgttat taagtgccat aacgctggaa gccgcattac aacaacgctg
     4441 ccgggaaaat attcatcgtt ttattcacaa cattatttat gacatccctg ggaacgcaac
     4501 tcaggcaata gaaaaaatta aacatattgg ctccagttcc ggctgcgaca tgctgtatgg
     4561 catggccgat ggttgtgcgc tgagccaaat ctacggaggg aattatgtca gttaaaatag
     4621 tcattaaacc gaatacctat tttgattctg tctcgctgat gtccatctcc acgcgtgcaa
     4681 ataaactcga cggtgtcgag caggcatttg tggcgatggc gaccgaaatg aacaaaggtg
     4741 tgctgaaaaa tttaggactg ctgacgccgg agctggaaca ggcgaaaaac ggcgacctga
     4801 tgattgtcat caatggtaaa tcaggtgcgg acaacgagca gttgctggcg gagattgaag
     4861 aactgttcaa caccaaagcg caaagcggct cgcacgaggc gcgttacgcc actattggca
     4921 gcgccaaaaa gcatatcccg gaaagtaacc tggcggtgat ttcggtcaac ggtctgtttg
     4981 ccgctcgcga agcgcgtcag gcgctgcaaa acgatctcaa cgtgatgctg ttttccgata
     5041 acgtctcagt tgaagatgaa ctggcgctca agcaactggc ccacgaaaaa gggctgctga
     5101 tgatggggcc agactgtggc acggcgatta tcaacggcgc ggcgctctgt tttggtaacg
     5161 ccgtgcgtcg cggcaacatc ggtattgttg gcgcatccgg caccggcagt caggagttga
     5221 gcgtccgcat tcatgaattt ggcggcggcg tttcgcaact gattggcacc ggcgggcgcg
     5281 acctgagcga gaaaatcggc ggcctgatga tgctcgacgc catcgggatg ctggaaaacg
     5341 atccgcaaac tgaaatcatt gcgcttatct ccaaaccgcc tgcgcctgcg gtggcccgca
     5401 aagtgctgga acgtgcgcgc gcctgccgca agccggtggt cgtctgcttc ctcgatcgtg
     5461 gcgaaacgcc ggtggatgag caggggctac agtttgcccg cggcaccaaa gaggcagcgc
     5521 taaaagcggt gatgctctcc ggcgtgaaac aggaaaatct cgacctgcat acgcttaacc
     5581 agccgttgat tgcggatgtg cgtgcgcgtc tgcaaccgca gcagaaatac attcgtggcc
     5641 tgttctgcgg cggcacgctg tgcgacgaaa ccatgttcgc ggtgatggaa aaacatggcg
     5701 atgtctacag caacattcag cccgatccgg aattccgcct gaaagatatc aaccgcagca
     5761 tcaaacacac cttcctcgac tttggcgatg acgacttcac caacggcaag ccgcacccga
     5821 tgattgaccc caccaaccgc atcagtcgct tgatcgaaga ggcgcgcgat ccagaagtgg
     5881 cggtgatcgt gatggatttt gtgctcggat ttggatcgca tgaagatccg gtcggctcca
     5941 ccatcgagac gatcaaagaa gcgaaagcga tcgccgctgc cgaaggacgc gagttgatca
     6001 ttctcgccta tgtgctgggt accgatcttg atacgccatc gttagaacaa caaagccaga
     6061 tgctgcttga tgccggagtg attctggcga gcagcagcac caataccgga ttgctggcgc
     6121 gtgaatttat ctgcaaaggg gaggaagcct gatgagccag tcactgttta gccaaccatt
     6181 gaacgttatt aacgtcggca tcgccatgtt tagcgatgac ctgaaaaagc agcatgtaga
     6241 agtgactcaa ctcgactgga cgccgccggg gcagggcaat atgcaggtgg tgcaggcgct
     6301 ggataacatt gccgattcgc cgctggcgga caaaatcgcc gccgctaacc agcaggcgct
     6361 ggagcgtatt atccagtcgc atccggtgct gattggtttt gatcaggcga ttaacgtggt
     6421 gccgggcatg acggcgaaaa ccattcttca cgccgggccg ccgatcacct gggaaaaaat
     6481 gtgcggcgcg atgaaaggcg cggtcaccgg agcgctggtg ttcgaaggac tggcgaaaga
     6541 tctcgacgag gcggctgaac tggcggcttc cggggagatc accttctcgc cgtgtcacga
     6601 gcacgactgc gtgggatcga tggcgggtgt tacctcggcc tcgatgttta tgcacatcgt
     6661 aaaaaacaaa acctacggca acatcgctta taccaacatg agcgagcaga tggcgaagat
     6721 tttgcgtatg ggcgctaacg accagagcgt gatcgaccgc ctcaactgga tgcgcgatgt
     6781 gcaggggcca atgctgcgcg acgcgatgaa aattatcggt gagatcgatc tgcgcttaat
     6841 gctggcgcag gcgctgcata tgggcgatga gtgccataac cgcaataacg ccgggacgac
     6901 gctgctgatt caggcgctga cgccggggat tattcaggcg ggttattccg tcgagcaaca
     6961 gcgcgaagtg tttgagtttg tcgccagcag cgactacttc tccggcccga cgtggatggc
     7021 gatgtgtaaa gcggcgatgg atgcggcgca tggcatcgaa tacagcaccg tggtcaccac
     7081 catggcgcgt aacggcgtcg agttcggcct gcgggtcagc ggcctgccgg ggcaatggtt
     7141 taccggcccg gcgcagcagg tgatcggccc gatgtttgcc ggttataagc cggaagattc
     7201 ggggctggat atcggcgaca gcgccatcac cgaaacctac ggtattggcg gatttgctat
     7261 ggcgaccgcg cccgctatcg tcgcgctggt gggcggcacg gtggaagaag ctattgattt
     7321 ctcccgtcag atgcgcgaaa tcaccctcgg tgaaaacccc aacgtcacca ttccgctgct
     7381 cggttttatg ggcgtgccgt cggcaatcga catcacccgc gtgggtagca gcggcattct
     7441 gccggtgatc aacaccgcca tcgcccataa agatgcgggc gtcggcatga ttggcgcggg
     7501 cattgtgcat ccaccttttg cctgcttcga gaaagccatt cttggctggt gcgaacgtta
     7561 cggcgtctga cctacatgtt catgccggat gcggcgtgaa cgccttatcc ggcctacgaa
     7621 tggcgcaaga atctgtaggc ctgataagcg tagcgcatca ggcatttgtc accattgccg
     7681 gatgcggcgt gaacgcctta tccggcctac gaatggcgca agaatctgta ggcctgataa
     7741 gcgtagcgca tcaggcattt gccaccactg ccggatgcgg cgtggacgcc ttatccggcc
     7801 tacgagtggc gcgagaatct gtaggcctga taagcgtagc gcatcaggca tttgtcacca
     7861 ttgccggatg cggcgtgaac gccttatccg gcctacgagt ggcgcgagaa ttgtaggcct
     7921 gataagcgta gcgcatcagg cagtctggcg ttggtcataa ccccatcacc ctctgtagcg
     7981 gacataacaa catgaaagag cttgtggtcg ttgccattgg tggcaacagc attatcaaag
     8041 ataacgccag ccagtcgatt gagcatcagg cggaggcggt gaaagccgtc gccgatacgg
     8101 tgctggaaat gctggcttcc gattacgaca ttgtgctgac ccacggcaac gggccgcagg
     8161 tcgggctgga tttacgccgt gcggagattg cccacgagcg cgaagggctg cccttaacgc
     8221 cgctggcgaa ctgtgtggca gatacgcagg gcggcatcgg ctatctgatc caacaggcgc
     8281 tgaataaccg gctggcgcgt cacggcgaga agaaagccgt caccgtggtg actcaggtgg
     8341 aagtggataa aaacgatcca ggttttgccc atcccaccaa gcccatcggc gcattcttta
     8401 gtgacagcca gcgtgacgaa ttacaaaagg caaaccctga ctggtgtttt gttgaagatg
     8461 ccgggcgggg ctatcgccgc gtggtcgcct cgccggaacc gaaacgtatt gtcgaagcac
     8521 ctgccattaa agcgctgatc caacaaggtt ttgtcgtaat tggcgcgggc ggcggtggaa
     8581 ttccggtagt gcgtactgac gcgggagatt accaaagcgt ggacgcggtt atcgacaaag
     8641 atctctctac cgcgctgctg gcccgtgaaa ttcacgccga cattcttgtg atcaccactg
     8701 gcgtcgaaaa agtgtgtatt cactttggca aaccgcagca gcaggcgctc gatcgggtgg
     8761 atattgccac catgacccgc tatatgcagg aagggcattt cccgcccggc agcatgttgc
     8821 caaaaatcat cgccagcctg acatttttag aacaaggcgg caaagaagtg attatcacca
     8881 cgccggaatg cctgcctgcg gcgctgcgcg gcgaaacggg cactcatatt attaaaacgt
     8941 aaggacgtaa gatgaaagaa agcaatagcc gccgtgaatt tctgagccag agcggtaaga
     9001 tggtcaccgc cgccgcgctg tttggtacct ctgtgccgct cgcccatgcg gcggtagctg
     9061 gcaccctaaa ctgcgaagcg aacaacacca tgaaaatcac tgacccgcat tactatctcg
     9121 ataacgtgct gctggaaacc ggttttgact acgaaaatgg cgtggcggtg cagacccgca
     9181 cggcgcgcca gaccgtggag attcaggacg gcaaaattgt cgccctgcgc gagaacaagc
     9241 tgcatccgga cgccacgctg ccgcactatg acgctggcgg taagctgatg ctgcccacca
     9301 cccgcgacat gcatattcat ctcgacaaaa ccttttacgg cgggccgtgg cgctcgctca
     9361 atcgtccggc aggcaccacc atccaggaca tgatcaaact cgagcagaaa atgctgccgg
     9421 aactgcaacc gtacactcag gagcgggcag aaaaactgat tgatttattg cagtcgaaag
     9481 gcaccaccat tgcccgcagc cactgcaata tcgaaccggt ttccggcctg aaaaatctgc
     9541 aaaatttgca ggcggtgctg gcgcgacgtc aggcgggctt tgagtgtgaa atcgtcgcct
     9601 tcccgcagca cggtttgctg ctgtcgaaat ctgaaccttt aatgcgtgaa gcgatgcagg
     9661 cgggggcgca ttacgtcggc ggcctggacc cgaccagtgt tgatggcgcg atggaaaaat
     9721 ccctcgacac catgttccag attgcgctgg actacgacaa aggcgtcgat attcacctgc
     9781 acgaaaccac tccggcaggc gtggcagcca tcaattatat ggttgaaacg gtagagaaaa
     9841 cgccacagct gaagggcaag ctgaccatca gtcacgcctt tgcgctggca acgctcaacg
     9901 agcaacaggt agatgaactg gcgaaccgga tggtggtgca acaaatttct attgcctcga
     9961 cggtgccgat tggcacgctg catatgccgc tcaaacagtt gcacgacaaa ggcgtaaaag
    10021 tgatgactgg cactgacagc gttatcgacc actggtcgcc ttatggtctg ggcgacatgc
    10081 tggaaaaagc caatctgtac gcgcagctct atattcgtcc taacgaacag aacctctccc
    10141 gttcgctgtt tctagccact ggcgatgtat tgccgctgaa tgaaaaaggc gagcgtgtat
    10201 ggccaaaagc gcaggatgac gccagctttg tgctggtgga cgcctcctgt tctgccgagg
    10261 cggtggcgcg tatctcgccg agaacagcaa cgttccataa agggcaactg gtgtggggga
    10321 gtgtggcagg ttgatgcgca tttgaaacgc ctgcaacggt gaatagtaag agatttaagc
    10381 cccagggagt ggggcaatct gaatatgagt gtgtcggcag aaatggacat tatctttgag
    10441 gaatatgccc ttattgacgt tgtgtagata actatttgtc tacgtgaaaa gatcatcagt
    10501 tttgccgatt ttagtctttt acagatagca aatatcacac ttacaggcgc tcgccttatg
    10561 gggaacgttg ctgctctgtt tcattcaggc tcgcggcatg ttggggctgg atcgggtggt
    10621 ttaaatattg gctatattca atggacgcgt tttgccgcga tgacatatca ggcgttgcca
    10681 aatacacata gctaatcagg agtaaacaca atgaagatca aagctgttgg tgcatattcc
    10741 gctaaacaac cacttgaacc gatggatatc acccggcgtg aaccgggacc gaatgatgtc
    10801 aaaatcgaaa tcgcttactg tggcgtttgc cattccgatc tccaccaggt ccgttccgag
    10861 tgggcgggga cggtttaccc ctgcgtgccg ggtcatgaaa ttgtggggcg tgtggtagcc
    10921 gttggtgatc aggtagaaaa atatgcgccg ggcgatctgg tcggtgtcgg ctgcattgtc
    10981 gacagttgta aacattgcga agagtgtgaa gacgggttgg aaaactactg tgatcacatg
    11041 accggcacct ataactcgcc gacgccggac gaaccgggcc atactctggg cggctactca
    11101 caacagatcg tcgttcatga gcgatatgtt ctgcgtattc gtcacccgca agagcagctg
    11161 gcggcggtgg ctcctttgtt gtgtgcaggg atcaccacgt attcgccgct acgtcactgg
    11221 caggccgggc cgggtaaaaa agtgggcgtg gtcggcatcg gcggtctggg acatatgggg
    11281 attaagctgg cccacgcgat gggggcacat gtggtggcat ttaccacttc tgaggcaaaa
    11341 cgcgaagcgg caaaagccct gggggccgat gaagttgtta actcacgcaa tgccgatgag
    11401 atggcggctc atctgaagag tttcgatttc attttgaata cagtagctgc gccacataat
    11461 ctcgacgatt ttaccacctt gctgaagcgt gatggcacca tgacgctggt tggtgcgcct
    11521 gcgacaccgc ataaatcgcc ggaagttttc aacctgatca tgaaacgccg tgcgatagcc
    11581 ggttctatga ttggcggcat tccagaaact caggagatgc tcgatttttg cgccgaacat
    11641 ggcatcgtgg ctgatataga gatgattcgg gccgatcaaa ttaatgaagc ctatgagcga
    11701 atgctgcgcg gtgatgtgaa atatcgtttt gttatcgata atcgcacact aacagactga
    11761 aaaaattaat aaataccctg tggtttaaca tattaacttc gctctccact taacttttta
    11821 gttaaggaga gcgaaataat atcaaagtag cagtaaaacc tataacgtaa atttaaattg
    11881 ttaaattaac gccctccagt acacaatact tcacacgtta gttatgagcg atttctgata
    11941 gtgcctggtt taatcagagc tttattatct gcgacgttta tttttattta agagagatcg
    12001 cgatgatatc attaaaagct ccgcacaata atttgatgcc atatacgcaa caaagcatac
    12061 ttaatacggt taaaaacaat cagttaccag aggatatcaa aagctccctg gtttcctgtg
    12121 tcgatatatt caaggttttg attaaacaat attatgatta tccttatgat tgtcgtgatg
    12181 atttagtcga tgacgacaaa ctcattcatc tcatggctgc cgtacgagac tgtgagtggt
    12241 cagacgataa cgcactcacc ataaatgtgc agtttaatga tttccctgga ttttatgact
    12301 ggatggatta ccctgatcac ccggttaagt ttgtttttca catacttgaa aatcaaaaag
    12361 ggacagtttg ggtatatgac caggatgatg cgtttcttga tattaaagca aacgtgcagg
    12421 ctggacgctt taccgggctt aagaaattag tgcagtttat cgattcagtg cgaacagatt
    12481 gcaaatgtat tcttcttgaa caccatatgc ctttgctgag aattttccct aaagggaaag
    12541 agtgtatgca tgtggaaaaa tggcttcgcg aaatgtcgtc tattccggag acagacgcgc
    12601 ccattaagca ggcgcttgcg catggattat tattacactt aaaaaatatc taccctgttt
    12661 ttcctgaatc actggtgatg ttattgctct ctgttctgga cgtaaaaaca tacagagatg
    12721 atgcgcgatt aaatgaatgg atatctaata gagtgcagga attaggtgac agatattatc
    12781 ctgtaaataa gcacgttaaa atccgctaca ctctttagtt cagaaagggt tccacctgca
    12841 aattgttatt ccacctgaga gtgaatcgca atgcaggtaa tcatttcatc tgtatatctg
    12901 tatgccaaca ggcattccat ctcttttttc gcctatattt tgttgtgatc ggcgacactt
    12961 cgcttaaaaa agcaccagta gtggtttcgc agccatgcgg tgtataaaaa atgatctcat
    13021 gcagatgttt tgtgaatgtg tgggttgaca ttcatatgaa aaaaatcata attccatcat
    13081 gtttgtgtgg ggtcttttct gtatcttacg catcgcactc aagcctgaca gaaaatatgc
    13141 tgtaaggctc atatcaaaaa ccgccattag ctcatcagga agagcagacg acaaccagga
    13201 ttgttgtatg gtacggggtt cgaggcctcg atggcggtcc aacttttcaa aactcttctg
    13261 aatcagatcc ccttattatc atccctgcaa agtggaacat tgccattgtt tggctattca
    13321 ggatggggca gaccaatgaa aaaagcccac acaggggaga gtgggctgaa atgggaagct
    13381 aaagactcaa gtaaacttat cggaaataag gaccacgcat tacgggggct atcatcgccg
    13441 atggccgtga ttcggcaaat caggttgatt acgtagagag taaattatct gctcaccgct
    13501 gcgtcacccc ttcgtaaatc aggcgtagcg cgaatacacc aataattgca ccaataaccc
    13561 gactggcaac gcgttgcata cgcccataag cacgacgcac agcgggcaaa gaaaacgcct
    13621 gactaagaaa aactcgccag ataattgatg cgagcacaat ccccgcccag gccattaaac
    13681 gtgcccatgt tggtgtttcg gcatttaatg ttactgagaa aatactgata aaaaataaaa
    13741 cggtttgcgg gttagagaga tcggtaatta atccgcggcg aaaaaagaca taccaggggg
    13801 cgctaatcgg ttgttgtagt gtgctcattt gcggtgttga ctggcggcgc atgctgcacc
    13861 acgcaaacca taagagataa gcgccgccga cgattctgat aagcgaaaaa atctcctcac
    13921 actgcgtaat tagcgttgca agaccaaaca aacccaaccc ggaataaaat gcatcgccca
    13981 gcgccacgcc cagcccggtc agcacccctg cgcgtcgacc ggaagccagg ctggtttgta
    14041 ctaccacaaa gagattggct cccggattaa aaaaagtaat cacgaacagt cctacggtca
    14101 ggtaaacggc atgcaaagga tccatagtga tttcatccat aaataagtga actaactgca
    14161 tcatatttct accaaaaata atcgggtgcg agagagatca caaagtgtct tatttccggt
    14221 tactggcgtt tatgccctga ctgaactaat tattaatcaa cccaataatg tcggtgggtg
    14281 atagtgtgat aacaactctg gagccgtaat atgaaaataa tctctaaaat gttagtcggt
    14341 gcgttagcgt ttgccgttac caatgtctat gccgctgaat tgatgactaa agcggaattt
    14401 gaaaaagtcg aatcgcaata tgaaaaaata ggtgatattt caaccagcaa tgaaatgtcg
    14461 accgcagatg cgaaagagga tttgatcaaa aaagcggatg aaaaaggggc tgatgtgttg
    14521 gtactgacct ccggtcaaac tgacaataag atccacggca cggcaaatat ttataagaag
    14581 aagtaattct gaatcctatg taaacatccc cgatgcgtaa gtttatcggt gatcatctat
    14641 tgaaatttat gccggataaa gcgttcgcgc tgcattcggc agttcagttt ttcagccgcc
    14701 gccagaacgt cgtccggctg atgcctaaat aattcgccgc tgctgtttta tcgccattaa
    14761 atttctccag tgcctgttgt ggtgtcagta agcgtggagc gggagttttc gacgactcgc
    14821 gcgccagttc cggcagtagc agctgcaaaa actgcggcgt taaatccggc gtcggttcca
    14881 cacttaaaaa tagcgccagt cgctccatca tattgcgcag ttcacgaata ttacctggcc
    14941 agtcgtagtg caccagcacg gtttcgcttg cctgtaatcc ctggcgtaat gcggcagaaa
    15001 acggggcgga gagcgccgcc agagacactt tcaaaaagct ttccgccagc ggcagaatat
    15061 ccgccacccg ctcacgcagt ggtggcaatt gcagacgcaa aatactcagc cgataaaaca
    15121 gatcacggcg aaactgccct tgccgcatat cttcttccag attgcagtga gtggcgctaa
    15181 tgacccgcac atccaccgga acaggctgat gcccgccgac gcgagtaacc tctttttctt
    15241 ccagcacccg taacaggcga gtctgcaaag gtagcggcat ttcgccaatc tcatccagaa
    15301 acagcgtacc gccgtgggca atttcgaaca gcccggcgcg acctccgcgt cgcgagccgg
    15361 tgaacgcccc ttcctcatag ccaaacagct ctgcttccag cagcgattcg gctatcgccc
    15421 cgcagttgac ggcaacaaac ggatgcgact ttttgccctg tcgcgcatcg tggcgggcaa
    15481 aatattcccg atgaatcgcc tgggccgcca gctctttacc cgtccccgtt tccccctcaa
    15541 tcaacaccgc tgcactggag cgggcataca gcaaaatagt ctgccgtact tgttccatct
    15601 gtggtgattg accgagcata tcgcccagca cgtaacgagt acgcagggcg ttacgggtag
    15661 catcgtgagt gttatggcgt aacgacatgc gcgtcatatc cagcgcatcg ctgaacgcct
    15721 ggcgcacggt ggcggcggaa tagataaaaa ttccagtcat tccggcttct tctgccagat
    15781 cggtaatcag ccccgcgccg accaccgctt cggtgccgtt agctttcagc tcattaatct
    15841 gcccgcgtgc gtcttcctcg gtaatgtagc tacgttggtc gaggcgcaaa ttaaaggttt
    15901 tttgaaacgc caccagcgct ggaatggttt cctgataggt gacaacgccg atagaagagg
    15961 tgagttttcc ggcttttgcc agtgcctgta acacatcgta gccgctcggt ttaatcaaaa
    16021 taactggcac tgacaggcgg cttttcaggt acgcgccgtt agagccagcc gcgatgatgg
    16081 cgtcacagcg ttcgtttgcc agtttcttgc ggatgtaggt cactgctttt tcaaagccaa
    16141 gctgaatagg ggtaatgttc gccaggtgat caaactcgag gctgatatcg cgaaacagct
    16201 cgaacaggcg cgttacagat accgtccaga taaccggttt gtcgtcatta agccgtggtg
    16261 gatgtgccat agcgcaccgc aaagttaaga aaccgaatat tgggtttagt cttgtttcat
    16321 aattgttgca atgaaacgcg gtgaaacatt gcctgaaacg ttaactgaaa cgcatatttg
    16381 cggattagtt catgacttta tctctaacaa attgaaatta aacatttaat tttattaagg
    16441 caattgtggc acaccccttg ctttgtcttt atcaacgcaa ataacaagtt gataacaaag
    16501 gatgggctat gtctctacac tctccaggta aagcgtttcg cgctgcactg actaaagaaa
    16561 atccattgca gattgttggc accatcaacg ctaatcatgc gctgttggcg cagcgtgccg
    16621 gatatcaggc aatttatctt tctggcggtg gcgtggcggc aggttcgctg gggctgcccg
    16681 atctcggtat ttctaccctt gatgatgtgc tgaccgacat tcgccgtatc accgacgttt
    16741 gttcgctgcc gctgctggtg gatgcggata tcggttttgg ttcttcggcc tttaacgtgg
    16801 cgcgcaccgt gaaatcgatg attaaagccg gtgcggcagg attgcatatt gaagatcagg
    16861 ttggtgcgaa acgctgcggt catcgtccga ataaagcgat cgtctcgaaa gaagagatgg
    16921 tggatcggat ccgcgcggcg gtggatgcga aaaccgatcc tgattttgtg atcatggcgc
    16981 gcaccgatgc tctggcggta gaggggctgg atgcggcgat cgagcgtgcg caggcctatg
    17041 ttgaagcggg tgccgagatg ttgttcccgg aggcgattac cgaactcgcc atgtaccgcc
    17101 agtttgccga tgcggtgcag gtgccgatcc tcgccaacat caccgaattt ggtgccacgc
    17161 cgctgtttac caccgacgaa ttacgcagcg cccatgtcgc aatggcgctg tacccacttt
    17221 cagcgttccg cgccatgaac cgcgccgctg aacatgtcta caacgtcctg cgccaggaag
    17281 gcacgcagaa aagcgtcatc gacaccatgc agacccgcaa cgagctgtac gaaagcatca
    17341 actactacca gtacgaagag aagctcgaca acctgtttgc ccgtagccag gtgaaataaa
    17401 aaacgcccgt tgattgtatt cgacagccga tgcctgatgc gtcgctgacg cgacttatca
    17461 ggcctacgag gtgcactgaa ctgtaggtcg gataagacgg atggcgtcgc atccgacaac
    17521 cgatgcctga tgcgccgctg acgcgactta tcaggcctac ggggtgcact gaactgtagg
    17581 tcggataaga cgcatagcgt cgcatccgac aaccgatgcc tgatgcgccg ctgacgcgac
    17641 ttatcaggcc tacggggtga actgaactgt aggtcggata agacgcatag cgtcgcatcc
    17701 gacaacaatc tcgaccctac aaatgataac aatgacgagg acaacatgag cgacacaacg
    17761 atcctgcaaa acagtaccca tgtcattaaa ccgaaaaaat ctgtggcact ttctggcgtt
    17821 ccggcgggca atacggcgct ctgcaccgtg ggtaaaagtg gcaatgacct gcattaccgc
    17881 ggctacgata ttcttgatct ggcgaaacat tgcgaatttg aagaagtggc gcatctgctg
    17941 atccacggca aactgccgac ccgtgacgaa ctcgccgctt acaaaacgaa actgaaagcc
    18001 ctgcgcggtt taccggctaa cgtgcgtacc gtgctggaag ccttaccggc ggcgtcacac
    18061 ccgatggatg ttatgcgcac cggtgtttcc gcgctcggct gcacgctgcc agaaaaagag
    18121 gggcataccg tctctggcgc gcgggatatt gccgacaaac tgctggcgtc gcttagctcg
    18181 attctccttt attggtatca ctacagccac aacggcgaac gcatccaacc ggaaaccgat
    18241 gacgactcca tcggcggtca cttcctgcat ctgctgcacg gcgaaaagcc atcgcaaagc
    18301 tgggaaaagg cgatgcatat ctcgctggtg ctgtacgccg aacacgagtt taacgcctcc
    18361 acctttacca gtcgggtgat tgcgggcacc ggctctgata tgtattccgc gattattggc
    18421 gcgattggcg cactgcgcgg gccaaaacac ggcggggcga atgaagtgtc gctggagatc
    18481 cagcaacgct acgaaacgcc ggacgaagcc gaagcagata tccgcaagcg cgtggaaaac
    18541 aaagaagtgg tcattggttt tggtcatccg gtttacacca tcgctgaccc gcgccaccag
    18601 gtgattaaac gtgtggcgaa gcagctctcg caggaaggcg gctcgctgaa gatgtacaac
    18661 atcgccgatc gcctggaaac ggtgatgtgg gagagcaaaa agatgttccc caatctcgac
    18721 tggttctctg ctgtttccta caacatgatg ggcgttccca ccgagatgtt cacaccactg
    18781 tttgttatcg cccgcgtcac cggctgggcg gcgcacatta tcgaacaacg tcaggacaac
    18841 aaaattatcc gtccttccgc caattatgtt ggaccggaag accgcccgtt tgtcgcgctg
    18901 gataagcgcc agtaaacctc tacgaataac aataaggaaa cgtacccaat gtcagctcaa
    18961 atcaacaaca tccgcccgga atttgatcgt gaaatcgttg atatcgtcga ttacgtcatg
    19021 aactacgaaa tcagctctaa agtggcctac gacaccgcac attactgcct gctcgacacg
    19081 ctcggctgcg gtctggaagc tctcgaatac ccggcctgta aaaaactgct ggggccaatt
    19141 gttcccggca ccgtcgtacc caacggcgtg cgcgtccccg gaactcagtt ccagctcgac
    19201 cccgtccagg cggcatttaa catcggcgcg atgatccgct ggctcgattt caacgatacc
    19261 tggctggcgg cggagtgggg ccatccttcc gacaacctcg gcggcattct ggcaacggcg
    19321 gactggcttt cgcgcaacgc ggtcgccagc ggcaaagcgc cgttgaccat gaaacaggtg
    19381 ctgaccgcaa tgatcaaagc ccatgaaatt cagggctgca tcgcgctgga aaactccttt
    19441 aaccgcgtcg gcctcgacca cgttctgtta gtgaaagtgg cttccaccgc cgtggtcgcc
    19501 gaaatgctcg gcctgacccg cgaggaaatt ctcaacgccg tttcgctggc gtgggtggac
    19561 ggtcagtcgc tgcgcaccta tcgccatgcg ccgaacaccg gcacgcgtaa atcctgggcg
    19621 gcgggcgatg ccacttcccg cgcggtacgt ctggcactga tggcgaaaac gggcgaaatg
    19681 ggttacccgt cagccctgac tgcgccggtg tggggcttct acgacgtctc ctttaaaggt
    19741 gaatcgttcc gcttccagcg cccgtacggt tcctacgtta tggaaaatgt gctgttcaaa
    19801 atctccttcc cggcggagtt ccactcccag acggcagttg aagcagcgat gacgctctat
    19861 gaacagatgc aggcagcagg caaaacggcg gcggatatcg aaaaagtgac cattcgcacc
    19921 cacgaagcct gtattcgcat catcgacaaa aaaggaccgc tcaataaccc ggcagaccgc
    19981 gatcactgca ttcagtacat ggtggcgatc ccgctgctgt tcgggcgctt aacggcggca
    20041 gattacgagg acaacgtggc gcaagataaa cgtatcgacg ccctgcgcga gaagatcaat
    20101 tgctttgaag atccggcatt taccgctgac taccacgacc cggaaaaacg cgccatcgcc
    20161 aatgccatta cccttgagtt caccgacggt acgcgctttg aagaagtggt ggtggagtac
    20221 cccattggtc atgctcgccg ccgtcaggat ggtattccga aactggtcga taaattcaaa
    20281 atcaatctcg cgcgccagtt cccgactcgc caacagcagc gcattctgga ggtttctctc
    20341 gacagaactc gcctggaaca gatgccggtc aatgagtatc tcgacctgta cgtcatttaa
    20401 gcaaacagcg gtaaggcgta agttcaacag gagagcatta tgtcttttag cgaattttat
    20461 cagcgttcga ttaacgaacc ggagcagttc tgggccgagc aggcccggcg tattgactgg
    20521 cagacgccct ttacgcaaac gctcgatcac agcaatccgc cgtttgcccg ttggttttgt
    20581 gaaggccgaa ccaacttgtg tcacaacgct atcgaccgct ggctggagaa acagccagag
    20641 gcgctggcgc tgattgccgt ctcttcggaa acagaagaag agcgcacctt tacctttcgt
    20701 cagctgcatg acgaagtgaa cgcggtggcc tcaatgttgc gttcattggg cgtgcagcgt
    20761 ggcgatcggg tgctggtgta tatgccgatg attgccgaag cgcatattac tctgctggcc
    20821 tgcgcgcgca ttggcgctat tcactcggtg gtgtttggtg gatttgcctc gcacagcgtg
    20881 gcggcgcgaa ttgatgatgc taaaccggtg ctgattgtct cggctgatgc cggagcgcgc
    20941 ggtggcaaaa tcattcccta taaaaaattg ctcgacgatg cgataagtca ggcgcagcac
    21001 cagccgcgcc atgttttgct ggtggatcgc gggctggcga aaatggcgcg cgtcagcggg
    21061 cgggatgtcg atttcgcgtc gttgcgccat caacacatcg gcgcgcgggt accggtggcg
    21121 tggctggaat ccaacgaaac ctcctgcatt ctctacacct ccggcacgac cggcaaacct
    21181 aaaggcgtgc agcgtgacgt cggcggatat gcggtggcgc tggcgacctc gatggacacc
    21241 atttttggcg gcaaagcggg cggtgtgttc ttttgtgctt cggatatcgg ctgggtggtg
    21301 gggcattcgt atatcgtcta cgcgccgctg ctggcgggga tggcgactat cgtttacgaa
    21361 ggattaccga cctggccgga ctgcggcgtg tggtggaaaa tcgtcgagaa atatcaggtt
    21421 agccggatgt tctcagcgcc gaccgccatt cgcgtgctga aaaaattccc taccgctgaa
    21481 attcgcaaac acgatctctc gtcgctggaa gtgctctatc tggctggaga accgctggac
    21541 gagccgaccg ccagttgggt gagcaatacg ctggatgtgc cggtcatcga caactactgg
    21601 cagaccgaat ccggctggcc gattatggcg attgctcgcg gtctggacga caggccgacg
    21661 cgtctgggaa gccccggcgt gccgatgtat ggctataacg tgcagttgct taatgaagtc
    21721 accggcgaac cgtgtggcgt caacgagaaa gggatgctgg tggtggaagg gccgctgccg
    21781 ccggggtgta ttcagaccat ctggggcgac gacggccgct ttgtgaagac ttactggtcg
    21841 ctgttttccc gcccggtgta cgccaccttt gactggggca tccgtgacgc tgacggttat
    21901 cactttattc tcgggcgcac tgacgatgta attaacgttg ccgggcatcg gctggggacg
    21961 cgcgagattg aagagagtat ctccagccat ccgggcgttg ccgaagtggc ggtggttggg
    22021 gtgaaagatg cgctgaaagg gcaggtggcg gtggcgtttg tcattccgaa agagagcgac
    22081 agtctggaag atcgtgatgt ggcgcattcg caagagaagg cgattatggc gctggtggac
    22141 agccagattg gcaactttgg ccgcccggcg cacgtctggt ttgtctcgca attgccaaaa
    22201 acgcgatccg gaaaaatgct gcgccgcacg atccaggcga tttgcgaagg acgcgatcct
    22261 ggagatctga cgaccattga tgatcctgcg tcgttggatc agatccgcca ggcgatggaa
    22321 gagtaggtta ttgtcggatg cgtcgcgcgg tgcatccggc actgtgtgcc gatgcctgat
    22381 gcgacgctga cgcgtcttat catgcctacg gacctgaacc gtaggtcgga taaggcgctc
    22441 gcgccgcatc cgacaccatg cgccgatgcc tgatgcgacg ctgacgcgtc ttatcatgcc
    22501 tacaggcctg aaccgtaggt cggataaggc gctcgcgccg catccgacac catgctcaga
    22561 tgcctgatgc gacgc
//
LOCUS       DAJOSY010000072        22515 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJOSY010000072 DAJOSY010000000
VERSION     DAJOSY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.072"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..522
                     /locus_tag="OHN27_002907"
     CDS             <1..522
                     /locus_tag="OHN27_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2287307.1"
                     /translation="KQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSN
                     MSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTS
                     QRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRP
                     LKQEPTEMIQATA"
     gene            complement(562..1776)
                     /gene="ydcO"
                     /locus_tag="OHN27_002908"
     CDS             complement(562..1776)
                     /gene="ydcO"
                     /locus_tag="OHN27_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCQ2287308.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMCIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FAFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSTPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1829..2365
                     /gene="sutR"
                     /locus_tag="OHN27_002909"
     CDS             1829..2365
                     /gene="sutR"
                     /locus_tag="OHN27_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCQ2287309.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHVYR
                     NGGKQTVHFHSLIHYPRS"
     gene            2438..4398
                     /gene="rlhA"
                     /locus_tag="OHN27_002910"
                     /pseudo
     CDS             2438..4398
                     /gene="rlhA"
                     /locus_tag="OHN27_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
     gene            complement(4490..4720)
                     /locus_tag="OHN27_002911"
     CDS             complement(4490..4720)
                     /locus_tag="OHN27_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCQ2287310.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4942..5096
                     /locus_tag="OHN27_002912"
                     /pseudo
     CDS             4942..5096
                     /locus_tag="OHN27_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            5142..5558
                     /gene="hicB"
                     /locus_tag="OHN27_002913"
     CDS             5142..5558
                     /gene="hicB"
                     /locus_tag="OHN27_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCQ2287311.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPKALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5637..7043
                     /locus_tag="OHN27_002914"
     CDS             5637..7043
                     /locus_tag="OHN27_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCQ2287312.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSQAALAHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7288..8433
                     /locus_tag="OHN27_002915"
     CDS             7288..8433
                     /locus_tag="OHN27_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2287313.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQEVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8451..9464
                     /locus_tag="OHN27_002916"
     CDS             8451..9464
                     /locus_tag="OHN27_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2287314.1"
                     /translation="MTYAVEFDNVSRLYGDVRTVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGITGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9465..10406
                     /locus_tag="OHN27_002917"
     CDS             9465..10406
                     /locus_tag="OHN27_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2287315.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIILIALYLAFVKRLGAFDAL"
     gene            10396..11190
                     /locus_tag="OHN27_002918"
     CDS             10396..11190
                     /locus_tag="OHN27_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2287316.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11212..12636
                     /gene="patD"
                     /locus_tag="OHN27_002919"
     CDS             11212..12636
                     /gene="patD"
                     /locus_tag="OHN27_002919"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCQ2287317.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADTAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12733..12828)
                     /gene="yncL"
                     /locus_tag="OHN27_002920"
     CDS             complement(12733..12828)
                     /gene="yncL"
                     /locus_tag="OHN27_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCQ2287318.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            13023..13196
                     /gene="ortT"
                     /locus_tag="OHN27_002921"
     CDS             13023..13196
                     /gene="ortT"
                     /locus_tag="OHN27_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCQ2287319.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            13282..13515
                     /locus_tag="OHN27_002922"
     CDS             13282..13515
                     /locus_tag="OHN27_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCQ2287320.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(13516..13965)
                     /locus_tag="OHN27_002923"
     CDS             complement(13516..13965)
                     /locus_tag="OHN27_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2287321.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(13962..14480)
                     /gene="mddA"
                     /locus_tag="OHN27_002924"
     CDS             complement(13962..14480)
                     /gene="mddA"
                     /locus_tag="OHN27_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCQ2287322.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTLAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14660..15697
                     /gene="curA"
                     /locus_tag="OHN27_002925"
     CDS             14660..15697
                     /gene="curA"
                     /locus_tag="OHN27_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCQ2287323.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            15895..16560
                     /gene="mcbR"
                     /locus_tag="OHN27_002926"
     CDS             15895..16560
                     /gene="mcbR"
                     /locus_tag="OHN27_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCQ2287324.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16596..18698)
                     /locus_tag="OHN27_002927"
     CDS             complement(16596..18698)
                     /locus_tag="OHN27_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCQ2287325.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEGMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDYYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNLGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            18940..20001
                     /locus_tag="OHN27_002928"
     CDS             18940..20001
                     /locus_tag="OHN27_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCQ2287326.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(20116..21615)
                     /gene="ansP"
                     /locus_tag="OHN27_002929"
     CDS             complement(20116..21615)
                     /gene="ansP"
                     /locus_tag="OHN27_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCQ2287327.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            21881..22480
                     /locus_tag="OHN27_002930"
     CDS             21881..22480
                     /locus_tag="OHN27_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCQ2287328.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEIWLNSQLTAEPYAFGEQLTLVDCYLCTMR
                     TWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         5732 a         5544 c         5823 g         5416 t
ORIGIN      
        1 aagcagaaac gcaaaataca gcgactgcat tcctgtatcg caaatatccg cagggactac
       61 cttcacaaag tcacaacggc cgtcagcaaa aaccacgcaa tgatagtcat tgaggatttg
      121 aaggtcagca acatgtcaaa gtcagcagcg ggtacggtca gccagccggg gcgcaatgtc
      181 cgggcaaaat caggtttaaa ccgttcgata ctggatcagg gctggtatga aatgcgccgc
      241 cagcttgcgt acaagcagct ctggcgtggc ggtcaggtgc ttgctgttcc gccagcgtat
      301 acaagccagc gttgcgcgta ctgtggtcat acagcgaaag agaaccgcct gtcacaaagt
      361 aaattcagat gccaggtatg tggatataca gcgaacgccg atgtaaatgg cgctcgcaac
      421 attttagcgg cggggcacgc cgttcttgcc tgtggagaga tggtgcagtc aggccgcccg
      481 ttgaagcagg aacccaccga aatgattcag gcgacagcct gaacgtagca gggatccacg
      541 tccttcaggg cgtggaggat gtcaatatcg gtttctgtca gcgattaaat tcaacaccac
      601 gtaacaaacg cctccggcaa ttaatcccca aaacgcagaa ccaatcccga ccagcgtcaa
      661 tccacttgcc gttaccagaa atgccaccac cgccgcgtcg cgctcacgct cattatgcag
      721 cgcctgatac aaactgccgc caatggtact caacagcgcc agacctgcca gcatctggat
      781 ccaacttacg ggcagggcag ccatcatccc ggtaatggca ctaccaaaca gacctgcgag
      841 caaatagaaa atgcctgcaa cggcagctgc cagccaacgt tgatctttat ccggatgcgc
      901 ttccgggctt tggcaaatag ccgcggtgat tgccgcaata ccgacggaat aaacgccgaa
      961 aggggaaaaa accagtgcca gcaatccagt aaatacaatt aatggcgaaa caggagttga
     1021 atatccggct gctttcattg ctgcgatacc cggtgcgttt tgcgatgcca tcgtcaccag
     1081 aaaaaggggg agtgcaacgc tcaggctgtg agcaaacgca aaatcagggg taatataagt
     1141 ggggagaacg ggtttaaaga caacatcagt tgtgacaacg tcaccttgcg cgataacgat
     1201 cacgatccca ataatcatcg cggcaattac cgcatagcgc ggcgcaacgg ccttggttgc
     1261 cagccatacc agcaacatac tgccacacaa cgtaaattga ccgtccagac tggcaaacgc
     1321 ctgtaaacca aagcgtaata aaatcccggc aagcattgcc gccgcaagcg agtgcggaat
     1381 aatgcacatc agacgagcaa agagtcccgt tatgccgcag aggactatta acgcgttggt
     1441 gacaataaaa acgccgatgg cttcgttaag tgttagtccc tgcaatccgg tgaccaacaa
     1501 agccgcgcca ggcgttgacc atgcggtgag aacaggtacg cgataccata atgtcagagc
     1561 cagcgtactg acgcccattg ccagccccag cgccgtcatc cagccagaga tttgtgcagt
     1621 ggtggctccg gcgacaatcg ctgcttgcca gattattgcc gctgaactgg cgtagccaat
     1681 taataccgcc agaaaccccg ccagtagcgt gggtggagga acagaaaaca gacgcatagt
     1741 cacctcgtgc gttatagcgt acaggcaccg tagcatttgt ccgttataac gcacaagtga
     1801 taaacttccg ttttgccgga ggagtcgcat ggaaaatctc gctcgctttt tatccaccac
     1861 acttaaacaa ctacgtcagc agcgcggctg gagtctttcg cgactggcag aagcgacggg
     1921 cgtttctaaa gcgatgttag ggcagattga gcgtaatgaa tccagcccga cggtcgcgac
     1981 attatggaaa attgccaccg ggttgaacgt gccattttcg acatttattt ctccgccaca
     2041 gtccgctacg ccttcagttt atgatccaca acagcaggcg atggtgatca cttcactgtt
     2101 tccttatgat ccacaactct gctttgaaca cttctcgatt cagatggcac ctggtgcaat
     2161 aagcgaatcg acgccccatg agaagggggt aatcgaacat gtcgtcgtca ttgatggaca
     2221 acttgatctg tgcgttgatg gggaatggca gagccttaat tgtggggaag gcgctcgatt
     2281 tgctgcagac gtcacgcatg tctatcgtaa tggtgggaag caaaccgtac attttcattc
     2341 cctcatccat tacccgcgca gttaagcggg aaaactattt cgcaacgtcg cgcttgtggc
     2401 taaaatagcc gccatttttc agctactgga taagaatgtg accgtatctt ctcatcgact
     2461 tgaactgtta agcccggcac gcgatgccgc cattgcccgc gaagctattt tgcacggtgc
     2521 tgatgctgtt tatatcggcg gccctggttt tggtgcccgt cataatgcca gtaatagcct
     2581 gaaagatatt gccgagttgg tgccgtttgc ccatcgttat ggtgcaaaaa ttttcgtcac
     2641 gcttaacacc attttgcatg atgatgagct ggaacccgcg caacggctga ttaccgacct
     2701 ctaccagacc ggcgtcgatg cgctgattgt tcaggatatg gggattctgg aacttgatat
     2761 tccgccgatt gaactgcacg ccagtacgca gtgcgacatt cgtacagtag aaaaagcgaa
     2821 gttcctctct gatgttggct tcacgcagat tgtgctggcg cgagagctga atcttgagca
     2881 gatccgcgcg attcaccagg ctacggacgc gaccattgaa ttctttattc atggcgcatt
     2941 gtgcgtggcc tattcgggtc agtgctacat atctcatgcg caaacagggc gtagcgccaa
     3001 ccgtggcgat tgttcacagg cgtgccgttt gccatacaca ttgaaagacg atcaggggcg
     3061 ggtggtttcc tatgaaaaac atctgcgtcg atgaaagata acgatcagac tgccaacctc
     3121 ggcgcgctga ttgatgctgg tgtacgctcc ttcaagattg aagggcgtta caaagatatg
     3181 agctacgtga agaatatcac cgcccattat cgccagatgc tggatgccat tattgaagaa
     3241 cgtggcgatc tgacgcgtgc ttcatcaggt cgtactgaac atttctttgt tccatcgacg
     3301 gaaaagactt tccaccgtgg tagcacagat tattttgtga atgcccgtaa aggcgatatt
     3361 ggcgcgttcg attcgccgaa atttatcggc ctgccggtag gcgaagtagt gaaagtggcg
     3421 aaagatcatc tcgatgttgc cgttaccgag ccactggcaa atggcgatgg cctgaacgtg
     3481 ttgattaaac gtgaagtcgt cggttttcgt gccaatacgg tcgagaaaac cggagaaaat
     3541 cagtaccgcg tctggcccaa tgaaatgcca gcagatttgc acaaaattcg cccgcatcac
     3601 ccactaaacc gtaatcttga tcataactgg cagcaggcac tgacaaaaac ctccagcgaa
     3661 cgtcgggtgg cggtagacat tgaactgggc ggttggcagg aacaattgat tctgactctc
     3721 accagtgaag agggggtcag cataacgcat acgctggatg ggcagttcga cgaagccaat
     3781 aacgccgaaa aagcaatgaa caatctgaag gatggtctgg caaaactggg gcaaaccctc
     3841 tattacgccc gcgatgtgca aattaatttg ccgggggcgc tgtttgtacc aaacagtctg
     3901 ttaaaccagt tccgccgtga agctgctgac atgctggatg ctgcgcgtct tgccagttac
     3961 cagcgcggca gccgtaaacc ggttgctgat cctgcgccgg tttatccgca aacgcatctg
     4021 agtttcctcg cgaacgttta caaccagaaa gcgcgtgaat tttatcatcg ctatggtgtg
     4081 cagctgattg acgcggcgta tgaggcacat gaagagaagg gcgaagtccc ggtgatgatc
     4141 accaagcatt gtctgcgctt tgcctttaat ttgtgcccga aacaggcgaa aggcaatatc
     4201 aaaagctgga aggctacgcc aatgcaactg gttaacggcg atgaagtatt aacgctaaag
     4261 tttgattgcc gcccatgcga gatgcacgtc attggcaaaa tcaaaaatca catactgaaa
     4321 atgcctttac cgggaagcgt agtggcatcc gtaagtccgg atgagctgct gaaaacattg
     4381 ccgaagcgaa aagggtaaaa cgccagtttt ctggttactc accactcatt gaatctgcat
     4441 gatattgcct gccgggtaag gcgtcacgcc gcatccggta tcaaacgact caacgttgtc
     4501 ccgcttctgg tttgcgcgat ttttgccagt aaaaatgctc gcgcaaacct tccgccgact
     4561 cttccgccac agcacgcaat tcatcgctgt ccgcttcatg acgcagctga tgatccacat
     4621 tctttaccca cacaaattca tgtcctttgt gccctgccat gagttgtcct gagaacagcg
     4681 cacacgttaa taagacaacc gataacgcct tcgtaaacat cctgccacct ttttcttacc
     4741 ttttgccgct atgatgccga tcgtttcttg aggttattat tcagttttgc aaattagcgc
     4801 aaagaaattc tggaatcttc cttcctgatt ttgcattgca ttctgccgtt gcggcgattt
     4861 agtgctattt tcgagcaaat tacacacgga ggtaaacact aatgtttact ttgttgatat
     4921 actcagcggc agggaggcga tgtgaaacaa agcgagttca gacgttggct cgaatctcag
     4981 ggcgtcgatg tagcgaatgg cagcaaccat ttgaaactca ggtttcatgg gaggcgcagt
     5041 gtcatgccgc gtcacccctg cgtaaagcaa tcctgaaaca actcggtttg agttaatcgc
     5101 caattaaaaa ggttaatgac atgcgaggga cagtcgaaat tatgcgttat cccgtcactc
     5161 ttacacccgc gccggaaggc ggttatatgg tttcttttgt ggatatccct aaagcgttga
     5221 cgcagggcga aactgtcgct gaagcgatgg aagcggcaaa agatgcttta ctgaccgcat
     5281 ttgattttta ttttgaagat aacgagctta tcccgttacc ttcgccatta aatagtcacg
     5341 atcactttat agaagtacct ttgagcgtcg cctctaaagt attgctatta aatgcttttt
     5401 tacagtcaga aatcactcag caagagttag ccaggcgaat tggcaaacct aaacaggaga
     5461 ttactcgcct atttaacttg catcatgcga caaaaatcga cgccgtccag ctcgcggcaa
     5521 aggcgcttgg caaagagtta tcgctggtga tggtttaatt acagttaacg aaaagttgtc
     5581 atttttaaca actgatatag actgccgaat catctgcaca taattacgat tcgataatga
     5641 aaaaatacca gcagcttgca gaacaattac gcgagcagat tgcgtcgggt atctggcaac
     5701 ccggcgatcg tttgccttcg ttgcgtgacc aggtggcgct ttcaggcatg agctttatga
     5761 ctgtcagcca tgcctatcag ttgctcgaaa gtcagggata tattatcgca cgaccgcagt
     5821 cgggttatta cgttgcgcca caggcaataa aaatgccgaa agcgccagtc attccagtca
     5881 ctcgagatga agcagtcgat atcaacacct atatttttga tatgttgcag gccagtcgcg
     5941 atccgtcggt agttccgttt gcctcggcct ttcccgaccc gcgacttttc cccctccaac
     6001 aactaaaccg ctcgctggcg caggtaagca aaaccgccac ggcgatgagc gtgattgaaa
     6061 atttgccgcc aggaaacgca gaactgcgtc aggctattgc ccgtcgctat gccttacagg
     6121 gcatcaccat ttctcctgat gaaattgtca tcactgccgg ggcgttagaa gcattaaacc
     6181 tcagtttgca agcggtaact gaaccgggcg attgggtgat agtagagaat ccttgtttct
     6241 acggtgcgtt gcaggcgctg gagcggctac ggctgaaggc gttatcggtg gcgacggatg
     6301 ttaaagaagg gatcgatctt caggcgctgg aactggcgtt gcaggagtat ccggtgaaag
     6361 cgtgctggct gatgactaat agccagaatc cactcggatt taccttaacg ccgcaaaaaa
     6421 aagcacaact ggtggcgttg ctcaatcagt acaacgtaac gctgattgaa gatgacgttt
     6481 acagcgaact ttattttgga cgggaaaaac cgctgcctgc gaaagcgtgg gatcgccacg
     6541 atggcgtttt gcattgctct tcgttttcga aatgtctggt gcctggtttt cgtattggtt
     6601 gggtcgccgc cggaaaacat gcacgtaaaa ttcaacgatt gcagttgatg agtacgcttt
     6661 ccaccagctc accgatgcag cttgcgctgg tggattacct ttccacgcgc cgatacgacg
     6721 cccatcttcg tcgcctgcgt cgccagcttg cggaacgtaa acaacgtgcc tggcaggcac
     6781 tgctgcgtta tctgcctgcg gaagtgaaaa ttcatcataa tgacagcggt tattttctct
     6841 ggctggagct tcccgaaccg ttagatgcgg gcgaattaag ccaggcggca ctggcgcatc
     6901 atatcagtat tgcgccgggt aaaatgtttt ctaccggtga aaactggtca cgttttttcc
     6961 gttttaatac cgcgtggcag tggggagagc gtgaagaaca ggcggtaaaa caattaggca
     7021 aacttattca agaacggctg taatagcgtt taatttaatt cctcttagat tgggtaatat
     7081 gaatttcgaa tagcagtcat atttcctaac tccttgacta tactccagaa gataacctta
     7141 cagacggcat aatgcgcggt agctcacaac ctgattaaat tttctcaggg gcgaaggtgt
     7201 gcctgcaagc cgccgtctat ggttaaacaa ggagatattt ttacggcacg gcggctgaac
     7261 aattaattac gacaggagta agaccttatg agcaagacat ttgcccgcag cagcctgtgt
     7321 gcgctcagca tgacaataat gaccgctcac gccgccgaac cacctaccaa tttagataaa
     7381 ccggaagggc gtctggatat tatcgcctgg ccgggataca tcgaacgcgg acaaactgat
     7441 aaacaatacg actgggtaac gcagttcgaa aaagagacag gctgcgcggt gaatgtgaaa
     7501 accgccgcga cttccgatga aatggtcagt ctgatgacca aagggggtta cgatctggtt
     7561 acggcatccg gcgatgcctc gctgcgtttg attatgggta aacgcgtgca gccgattaat
     7621 accgcattga ttcccaactg gaaaacgctc gatccgcgcg tggttaaagg cgactggttt
     7681 aacgtgggcg gcaaagtcta tggcacgcca tatcaatggg gtccgaacct gctgatgtac
     7741 aacactaaaa ccttcccgac gccgccggat agctggcaag aggtttttgt tgagcaaaat
     7801 ctgccggacg gcaagagcaa taaaggccgc gttcaggctt atgatggccc tatctatatt
     7861 gcggacgctg cgttgttcgt taaagccact cagccgcagt tgggcatcag cgatccgtat
     7921 caactcaccg aagaacagta ccaggcggtg ctgaaagtgc tgcgcgctca acacagtttg
     7981 atccatcgct actggcatga cactaccgtg caaatgagcg atttcaaaaa cgagggtgtg
     8041 gttgcttcca gtgcctggcc ctatcaggcc aacgccctga aagccgaagg ccagcctgtt
     8101 gctaccgttt tcccgaagga gggtgttacc ggttgggctg ataccaccat gctgcatagc
     8161 gaagcgaaac atccggtttg cgcctacaaa tggatgaact ggtcattaac gccaaaagtg
     8221 cagggcgatg tggcggcctg gtttggctcg ttaccggtag tgccggaagg gtgtaaagcc
     8281 agtccgttat taggcgaaaa aggttgtgaa accaacggtt ttaactattt cgacaaaatc
     8341 gccttctgga aaacgcctat agcagaaggg ggcaagtttg ttccctacag tcgctggacg
     8401 caggattaca ttgccattat gggcggtcgc taacttcgct ggggtgcttt atgacgtacg
     8461 cagtggagtt tgacaacgtc tcgcggttgt acggtgacgt gcgcacagta gatggcgtca
     8521 gtattgcgat aaaagatggt gagttcttct ctatgctggg gccgtccggc tccggcaaaa
     8581 ccacctgcct gcgcctgatt gctggcttcg aacagctttc cggcggggct atctctatct
     8641 ttggtaaacc cgccagcaat ctgccaccgt gggagcggga cgtgaatact gtctttcagg
     8701 actacgcgct atttccgcat atgtcgattc ttgacaatgt cgcctatggg ctgatggtca
     8761 aaggcgtgaa taaaaagcag cggcacgcaa tggcgcaaga ggcgctggag aaagtggcgt
     8821 tggggtttgt acatcaacgt aaaccgtcac aactttctgg tggtcagcgc cagcgggttg
     8881 ctatcgccag agcattggtg aatgaaccgc gcgtattgct gttggatgaa ccgctcggcg
     8941 cactggatct caaattgcgt gagcagatgc agctggaact gaaaaaactg caacagtctc
     9001 tcggtatcac ttttatcttc gttacccacg atcagggcga agcgttatcg atgtccgatc
     9061 gtgtggcggt tttcaataat ggacgcattg agcaggtcga ttccccgcgc gatctctata
     9121 tgcgcccgcg cacgccgttt gttgccgggt tcgttggtac atcgaatgtt tttgatggac
     9181 tgatggcaga gaaactttgt ggcataacgg gaagcttcgc cctgcgaccg gaacatatcc
     9241 gcctcaacac ccctggtgaa ctgcaggcca atggcacgat ccaggcggtg caatatcagg
     9301 gcgcggcaac tcgttttgaa ctgaaattga acggcggtga aaaactgctt gtgagtcagg
     9361 ccaatatgac aggcgaagaa ctgcctgcca cgctcacgcc cggacaacag gtgatggttt
     9421 cctggtcgcg tgatgtgatg gtgccgctgg ttgaggagag gtgaatggcg atgaatgtat
     9481 tgcaatcacc ttcacgtcca ggtctgggta aggtgtccgg tttcttctgg cgtaatccgg
     9541 ggctggggct gtttttactg ctgcttggcc cgctaatgtg gtttggcatt gtctatttcg
     9601 gctcgctgct gacactgtta tggcagggat tttatacttt tgacgatttc accatgtcgg
     9661 taacgccgga actgacgctg gcgaatatcc gtgcgctgtt taatccggcg aattacgaca
     9721 tcattctccg cacgctgact atggctgtgg cagtcactat cgccagcgcc attctggctt
     9781 ttccaatggc gtggtatatg gcgcgctata ccagcgggaa aatgaaagcg tttttttata
     9841 ttgcggtaat gttgccgatg tgggcgagct acattgttaa agcctatgcc tggacgttat
     9901 tgctggcaaa agatggcgtg gctcagtggt ttttacaaca tcttgggctg gaaccactgc
     9961 tgactgcgtt ccttacatta cctgcggtgg gcggaaatac gctgtcaact tccgggctgg
    10021 ggcgctttct ggtgtttctc tatatctggt tgccgttcat gatcctgccc gttcaggcgg
    10081 cgcttgagcg tttgccgccg tcattgttgc aggcgtcggc tgatctcggc gcacgtccac
    10141 gacaaacctt tcgctatgtg gtgctgccgc tggcaatccc gggtattgcc gctggctcta
    10201 tctttacctt ctcactcaca ctgggcgatt ttatcgtccc gcagctggtt ggtcctccag
    10261 gatattttat cggcaatatg gtttattccc agcagggggc gattggcaat atgccgatgg
    10321 cggcggcatt caccctggtg ccgattattc tcatcgcact gtacctggcg ttcgtgaaac
    10381 gtctgggagc gttcgatgca ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg
    10441 ggcggcgttg ttttcctaca ttttcccatc ctgataatcg ccgcctatgc gtttaacact
    10501 gaagatgcgg cgtttagttt tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca
    10561 gcacagcgta gtgatattct tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg
    10621 acattaattg cgctggtgtt agggacgctg gcagctgccg cgctgtggcg acgagacttt
    10681 ttcggcaaaa acgccatttc gctgttactg ctgctgccca ttgcgctgcc gggcattgtc
    10741 actggtctgg cgttattaac cgcctttaaa accatcaatc tggagccggg atttttcacc
    10801 atcgtggtcg gtcatgcgac tttttgtgta gttgtggtgt ttaacaatgt catcgcccgt
    10861 tttcgccgca cctcctggag tctggttgag gcgtcaatgg atcttggggc caatggctgg
    10921 caaaccttcc gctacgtagt gttgccgaat ctcagttcgg cgttactggc aggaggaatg
    10981 ctggcgtttg ccttgtcgtt cgatgaaatc atcgttacga cctttacggc aggtcatgaa
    11041 cgaacgttac cgttgtggtt gctcaatcag cttgggcgac cgcgtgatgt accggtaact
    11101 aacgtggtgg cactgctggt tatgttggta acaaccttgc cgatcctggg ggcctggtgg
    11161 ctaacccgcg aaggcgacaa tggtcaataa ccactgatac aggaatatgc tatgcaacat
    11221 aagttactga ttaacggaga actggttagc ggcgaagggg aaaaacagcc tgtctataat
    11281 ccggcaacgg gggacgtttt actggaaatt gccgaggcat ccgcagagca ggtcgatgct
    11341 gctgtgcgcg cggcagatac agcatttgcc gaatgggggc aaaccacgcc gaaagtgcgt
    11401 gcggaatgtc tgctgaaact ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa
    11461 ctggagtccc gtaattgtgg caaaccgctg catagtgcgt tcaatgatga aatcccggcg
    11521 attgtcgatg tttttcgctt tttcgcgggt gcggcgcgct gtctgaatgg tctggcggca
    11581 ggtgaatatc ttgaaggtca tacttcgatg atccgtcgcg atccgttggg ggtcgtggct
    11641 tctatcgcac cgtggaatta tccgctgatg atggccgcgt ggaaacttgc tccggcgctg
    11701 gcggcaggga actgcgtagt gcttaaacca tcagaaatta ccccgctgac cgcgttgaag
    11761 ttggcagagc tggcgaaaga tatcttcccg gcaggcgtga ttaacatact gtttggcaga
    11821 ggcaaaacgg tgggtgatcc gctgaccggt catcccaaag tgcggatggt gtcgctgacg
    11881 ggctctatcg ccaccggcga gcacatcatc agccataccg cgtcgtccat taagcgtact
    11941 catatggaac ttggtggcaa agcgccagtg attgtttttg atgatgcgga tattgaagca
    12001 gtggtcgaag gtgtacgtac atttggctat tacaatgctg gacaggattg tactgcggct
    12061 tgtcggatct acgcgcaaaa aggcatttac gatacgctgg tggaaaaact gggtgctgcg
    12121 gtggcaacgt taaaatctgg tgcgccagat gacgagtcta cggagcttgg acctttaagc
    12181 tcgctggcgc atctcgaacg cgtcagcaag gcagtagaag aggcgaaagc gacagggcac
    12241 atcaaagtga tcactggcgg tgaaaagcgc aagggtaatg gctattacta tgcgccgacg
    12301 ctgctggctg gcgcattaca ggacgatgcc atcgtgcaaa aagaggtatt tggtccagta
    12361 gtgagtgtta cgcccttcga caacgaagaa caggtggtga actgggcgaa tgacagccag
    12421 tacggacttg catcttcggt atggacgaaa gatgtgggca gggcgcatcg cgtcagcgca
    12481 cggctgcaat atggttgtac ctgggtcaat acccatttca tgctggtaag tgaaatgccg
    12541 cacggtgggc agaaactttc tggttacggc aaggatatgt cactttatgg gctggaggat
    12601 tacaccgtcg tccgccacgt catggttaaa cattaaggat aatattgcag atcgtaagag
    12661 tatgagatga tcttgcgtac tgtcagagca ctgatttctc tgacagtacg tgctgcgtgc
    12721 aaaatcacta catcaaataa accagccaaa tctcatagag ataagagtaa acaaaccaac
    12781 agcagcaaag acatttatca gtactacagt tctactggaa acattcattt ttgccacctt
    12841 ttatcattgc ccctatgtgt atagtacggg ttaagaaaat ccgcaataat actcattctg
    12901 aagatggata agggcaagtt gctgtttgat gattttttct caaactggtg aaaaaacttg
    12961 atgcacgtca aaaaatgacg catatttgcg cgcgttttat tcatctggct ggacgcccgt
    13021 acatgtctct ctatcaacac atgcttgttt tttatgcggt tatggcagca atcgcatttc
    13081 ttatcacctg gtttctttct cacgataaga aacgcatccg tttcttaagc gcctttctgg
    13141 tgggggcaac atggccgatg agtttcccgg tggcgctgtt gttttcactg ttttaaatct
    13201 tactcactgg cttgacggca ccacgcagac ttatatcatt tggatgaatc gataaatttc
    13261 acaagtggct aaggagaaag tatgtcgcat ctggatgaag tcatcgcgcg cgtggatgcc
    13321 gcgattgaag agagcgtcat tgcccatatg aacgaattat taatcgccct gagcgatgac
    13381 gcggagttaa gtcgggaaga tcgctacacc cagcagcaac gtctgcgcac agcgatcgcc
    13441 catcacggtc gcaagcataa agaagatatg gaagcgcgcc acgaacagtt aaccaaaggc
    13501 ggcaccatcc tctgattaaa atgagcgtct ggctaccaac caggcgccaa ccacaagtaa
    13561 aattgcgcca caaattgggc cgaacaacgc ccgtagtggt acgccatgac tgcggaagat
    13621 atccagcatc agaccgccaa tcagctgact ggcgaccagt acagcaatag ttgtcgccgc
    13681 tcccacattc tgatatccgc tgatactggc gaagacaaaa aacgatccca gcaagccagg
    13741 tataagtgtc caccagcgca cgctggacac cagttcgcca aaccccgcca tgccttgttt
    13801 aaaccataaa atagaaacaa agagcacaat ccccaccagt gagttcagca acatggcgat
    13861 gagaatggta gaggaggtct gggtgatgcg caccatcagc gtgttctgga caaccagacc
    13921 aattccggcg gcaatcaaaa aagcaagggt gagcgactga ttcatccaat cgcgtccggt
    13981 tcagtgcgct cgtcgagttg caactgcata aatgtgagat ccagccaacg accaaattta
    14041 gtgcctacct gcggcatttg cgcggtgacg acaaatccca gcgactggtg gagatgcagc
    14101 gaggcctgat tttgcgattc gatcccggcg accatgacat gcttcccgca atcccgcgct
    14161 tcatcaatca atcggcttaa caatttacgc cccagacctt tgccctgatg atcgggatgg
    14221 acataaaccg aatgttccac ggtatggcga aaaccatcga aactacgcca gtcgccaaac
    14281 gaggcatatc ccgtcactac gccatcttct tcgctcacca gcaccggata accagctaaa
    14341 gtccgcgctt caaaccaggc aatgcggtta tctgcatcca ccgtttggtc attccagata
    14401 gccgccgtat acaacacggc gtggttataa atttccgcaa tagcagcaca atcggctttg
    14461 cgggcaaaac ggatggacat gtctggcctc gataaaataa tgatgatgat gttatcaata
    14521 ctatcgataa cagcccgaag caacggattc ctcgatgaaa ttattgcgtg ttcagagggg
    14581 atgtgacggc tgcaaacaaa accaatcccg tgagcctgct actattgatg gattcatgcc
    14641 aaaagcacag gagagcatta tggggcaaca aaagcagcgt aatcgacgtt gggttctggc
    14701 ctcgcgtcca catggcgcac cggttccgga gaatttccgt cttgaagaag atgatgtcgc
    14761 cacaccgggt gaaggacagg tgttactgcg cacagtttat ttgtccctgg acccgtatat
    14821 gcgtggacgt atgagcgatg agccatctta ttcaccgcct gttgatattg gcggcgtgat
    14881 ggtcggcggc acggtgagcc gtgttgtgga gtcgaatcat cctgattatc agcctggcga
    14941 ctgggtgctg ggctacagtg gatggcagga ctatgacata tccagtggtg atgatctggt
    15001 gaaacttggc gatcatccgc aaaatccatc gtggtcgctg ggtgtgctgg ggatgccagg
    15061 ctttaccgct tatatggggc tgctggatat cggtcagcct aaagagggcg aaacgttggt
    15121 ggtagctgcg gcgacaggtc ctgtgggagc aacggtgggg caaatcggca aacttaaagg
    15181 ttgcagggtg gtgggggttg ccggtggcgc ggaaaaatgc cgccatgcta tcgaggtgct
    15241 gggcttcgat gtttgtcttg atcaccacgc ggatgatttt gccgaacaac tggcgaaagc
    15301 gtgcccaaaa ggtattgata tctattatga aaacgtgggc ggtaaggtat ttgatgcagt
    15361 gctaccatta cttaatacat ctgcgcgcat tcccgtttgt ggcttagtga gcagctataa
    15421 cgctacagag ctaccacccg gtccggatcg tttacccctg ttgatggcaa cggtgctgaa
    15481 aaaacgtatt cgcttgcaag ggtttattat cgctcaggat tatggtcacc gcatccatga
    15541 gtttcagaag gagatggggc aatgggtgaa agaggataaa atccactacc gcgaagatat
    15601 tactgatggt ttagagaatg cgccacagac gtttatcggc ctgctgaagg gtaaaaactt
    15661 cggcaaagtg gtgatccgcg tggcgggtga tgattaaagt ttcaacggcg gcgtaagccg
    15721 ccataaaaat ccaggcaaaa agcttatttt cagctttaat taactaacca cgtcattgtt
    15781 ctgctgctaa taacaacaaa ggtgagtggt ttatgattat tcactttaat acaccaggtg
    15841 aactccttct gccatgcagg cagggttgga cagaaaacac gcgaaggaat taaaatgcct
    15901 ggaacgggaa aaatgaaaca tgtcagtttg actctgcagg ttgagaacga cctgaaacat
    15961 cagcttagta ttggcgcatt aaaacctggc gcacgcctga ttactaaaaa tctggcggag
    16021 caattaggta tgagtattac acctgtgcgt gaagcattat tacgtctggt ttcggtgaat
    16081 gcgctttctg tcgcacctgc acaagcattt acagttccgg aagtggggaa acgtcaatta
    16141 gacgaaatca atcggatccg ctacgaactg gaattaatgg cagttgctct ggctgttgaa
    16201 aacctcaccc cgcaagacct tgcggaactc caggaattgc tcgagaaatt acagcaggcg
    16261 caagaaaagg gcgacatgga acaaatcatt aatgtaaaca ggctatttcg cttagcgatt
    16321 tatcatcgct caaatatgcc catcctgtgt gagatgattg agcaactgtg ggtcaggatg
    16381 gggcctggtt tacattatct ctatgaagcg attaatccag cggaattacg ggagcatata
    16441 gaaaactatc atctgttact cgctgcgtta aaagcaaaag acaaagaggg atgcagacat
    16501 tgtcttgctg aaattatgca acaaaatatt gctattttat accagcaata caatcgttaa
    16561 agtaaatgcc cggcaaatac cgggcatcaa ggtaattact caaatctcca cgcaatattc
    16621 atgccgacac cataatttcg tccgggcgaa ggttcgtaat atcgcccgtt tgactcattg
    16681 acaatgacag aaccaacgta ttctttatcg aataaattat cgacacgacc aaataaatcc
    16741 acagttaaat tgtggtaatt gtatttatac ccggtgaata agccgacgag agtataagac
    16801 ggcgcttttg ccgtattttc atcatctgcc ataatatcgc ccatataacg cgcttccgtg
    16861 cctgcatacc aaccatcttc cggtacataa cctatcgacg caaagcccat attacgggcg
    16921 atccctggca tccgattacc gttacaatcc tgttcattgc aaacattgct gcgataggtc
    16981 gcatcaagcc aggtccagga cgcgtttacg cgaaaatctc ctgcgaaacg ttgatcccat
    17041 gccagttcag cgccttgacg acgggtcttt ccggcatttt tgtaagtcgt acgcccaccg
    17101 ctactgctat cgacaacaat ttcatcatca gtgtcggtct gaaacaatgc gagactaagc
    17161 agcccatcac caatacgcgt tttactgccg atctcaattg tatcgttggt ggatggtttt
    17221 aaacctaagt tcataccgct ttgcccatca gcacgataag acagctcatt aatcgtcggc
    17281 gtttcaaaac ctcgcccggc tgccagatag atattccagg catcggtcat tgcatatttt
    17341 aacgaaccgg caggtagcca tttatgataa ctggcatcac cgctgtcatc gccgttaccc
    17401 ggagtaacgt aatagtcgtt ggaatcaaac cacacggagc tgtagcgcac gccagcatcc
    17461 agcgacagtt tttcgctcag ctgccactgc gtctgtaaat agggatcgat gttccacatc
    17521 agattgcgtt cgtcgcgacg caactcacct ttttgcccgt actccggcat gccgctattc
    17581 aggcggaagt tattgtagcc cttgcggttt tcactcatgt tttcgtagtt caggccggta
    17641 gtgaacgtga ccggaacgcc cagttcacca cggtgtgtcc agcggctgtc tattccctgg
    17701 taatggcgtt gcagggtaat cacgccgccc gcatgtgacg ggttaagttg tggtgccatg
    17761 ggtattgact ggtactgggt cgtttctcgc tctccggcat acatcatcac actcatatca
    17821 tcccgcgaac tcaggctacg ttcatagcgc aacccagcct gagtttgctt gatggttttt
    17881 cgcgtgtcgt actgttctgc acgaggcgct tgttgtggat tcgccttcca ttctgctttg
    17941 gttagcccac ctgggtcatc tgctttgata tccacactat tgaaaatcag gcttaatttg
    18001 ctggcttcat caatgcgtac gcccagtttg gcattggcta aatttttctg tgcgccacta
    18061 tggtcacgat agccgtgggt cgtaaaacgc gtggttgaga cggtgtaatc aacatcgccg
    18121 ggttgtgtgc cgtctcccgt tgcgcccgtt gctttcagcc catagcgcca gctgccaaaa
    18181 ctgccgtagt aactactggc ttcaatggtt ggtggctgtt gtccggtctg ggtggtgaca
    18241 ttcattacac cgccagacgc gttgccatac agggcagaaa aggggccacg cagcacttcc
    18301 acattttgca cactgcttaa atcgatgttg gatgtttgcc cttgcccgtc gggcatggtg
    18361 gcgggaatac cgtccacata caggcgaata ccgcgaattc cgtaagtgga gcgggagcca
    18421 aatccgcgaa tcgacagctg taaatcttgc gcatagttct gccgattttg tacctgcaaa
    18481 ccaggcacgc tggtcagtga ttcggacaag ttaatgcgcg gtgttgccag gcgcatcccc
    18541 tcgccatcca ccacgcttac tgctgctggg gtatccagtt ctgaaaccac ctgcggtgcg
    18601 gcactgacaa tcatggtttg ttcatcagcg gcaaaaacaa cgggggaaag ggcaagtagt
    18661 gcgggcaaaa cggtctgtcg gacggaaaaa atcttcatga aaaaagccag gttaagaatg
    18721 ggaaaacgcc gttatggtaa tgaaattgta aatttatgga aaacgaaacg gcacgatacg
    18781 ttaagtaatt gagaaaattg tagtcgtaac ggcaagaaat gctccacatt tgagaaaata
    18841 atgattacca ttcccattta taacaagagc gtaacgatga ttacgcttag cgaagcattg
    18901 tgaagcagca aaaatatcgg ttcatcaaag ggagtcgtca tgcatttacg tcatctgttt
    18961 tcatcgcgcc tgcgtggttc attactgtta ggttcattgc ttgttgcttc atcattcagt
    19021 acgcaggccg cagaagaaat gctgcgtaaa gcggtaggta aaggtgccta cgaaatggct
    19081 tatagccagc aagaaaacgc actgtggctc gccacttcgc aaagccgcaa actggataaa
    19141 ggtggcgtgg tttatcgtct tgatccggtc actctggaag tgacgcaggc gatccataac
    19201 gatctcaagc cgtttggtgc caccatcaat aacacgactc agacgttgtg gtttggtaac
    19261 accgtaaaca gcgcggtcac ggcgatagat gccaaaacgg gcgaagtgaa aggccgtctg
    19321 gtgctggatg atcgtaagcg cacggaagag gtgcgcccgc tgcaaccgcg tgagctggta
    19381 gctgatgatg ccacgaacac cgtttacatc agtggtattg gtaaagagag cgtgatttgg
    19441 gtcgttgatg gcgagaatat caaactgaaa accgccatcc agaacaccgg taaaatgagt
    19501 accggtctgg cgctggatag caaaggcaaa cgtctttaca ccactaacgc tgacggcgaa
    19561 ttgattacca tcgacaccgc cgacaataaa atcctcagcc gtaaaaagct gctggatgac
    19621 ggcaaagagc acttctttat caacattagc cttgataccg ccaggcagcg tgcatttatc
    19681 accgattcta aagccgcaga agtgttagtg gtcgataccc gtaatggcaa tattctggcg
    19741 aaggttgcgg caccagaatc actggctgtg ctgtttaacc cagcgcgtaa tgaagcctac
    19801 gtaacgcatc gtcaggcagg taaagtcagt gtgattgacg cgaaaagcta taaagtggtg
    19861 aaaacgttcg atacgccgac tcatccaaac agcctggcgc tgtctgccga tggcaaaacg
    19921 ctgtatgtca gtgtgaaaca aaaatccact aaacagcagg aagctaccca gccggacgat
    19981 gtgattcgta ttgcgctgta atagatgtgg cgagggtgaa aacggagaga cttgcctgac
    20041 ttttatcacg ccgcatgcgg cacaccttcg tcagtaatct aaatttgccc ggcacgtagc
    20101 cgggcaattt tgcaattagc tcgccgtttc aggcttaaac acaatttcct gtttttcttc
    20161 atcttcctcg acgactggcg cagtgctgtg aatttcagca acgcgtttgc gcacaccaaa
    20221 ccagcctata accagcagaa taccgataat cggcagcgcc gcgatagtat aagtcccgtt
    20281 cgggtaatcg aacgccatca gcaccaggac actcagtaaa aacagtaatg tcagccagga
    20341 agtgaagggc gcgccaggca gtttaaaact gacatccgct gctttgcctt gtttaattgc
    20401 tttacgcagg cgcatctggc acacgatgat aaacgcccat gaagcgatga ttcccagcga
    20461 cgcgaagttc aacacaatct caaatacgcg cgacggcacc agatagttga ggaatacgcc
    20521 gacgacatac acaactagtg tcgccagaat cccggcatac ggcacatgct gacgactcat
    20581 tttcgccata aaactcggtg cggaaccgcc catcgccatt gagcgcagaa tacgtccggt
    20641 gcagtacaga cctgagttca ggctggagag ggcagcggtc agcaccacaa tgttcataat
    20701 gctgccgata tatggcacac ccagtttaga gaaaaacgtc acgaacggac tttgccccgc
    20761 ctgatacgcg ctccacggca atagcataac cagcaacacc acggagccga cgtaaaacag
    20821 gccaatacgc caaatcacgc tgttaatcgc tttaggcacc atggtctgcg gatctttaca
    20881 ttctccggca gctgtaccca ccatttcaat ggaggcaaaa gcaaacacta cgccctgaat
    20941 caacaccaga gcaggcagca aaccgtgggg gaagaagccg ccattatcgg taattaaatg
    21001 aaagccagtg gtgttgccat ccagcggctg accactaccg aggaacactg tacccacgac
    21061 caaaaaggtc acaatggcga gcactttaat aagcgcaaac cagaactcca tctccgcaaa
    21121 ccatttcaca ccgatcatat tcatggtgcc aacgatggta agtgcagcga gcgcaaagac
    21181 ccactgcggc acgccgccaa acgcacccca gtaatgcata tacagagcga cggcggtaat
    21241 atcaacaatc cccgtcatcg cccagttgat gaaatacatc cagccagcaa cataagcggc
    21301 tttctcaccc aaaaactcac gggcataaga gacaaaactg ccactggaag ggcggtgtaa
    21361 caccagctca cccaatgcac gtagaataaa aaatgaaaac aggccgcaga tcaaatagac
    21421 cagtgccagt gcgggccccg ccatttgcag tcgggctcct gcacctaaaa acaagccggt
    21481 gccaatcgcg ccgccaatgg cgatcatctg tacctggcga ttgcccatcg ctttgtgata
    21541 cccctcttcg tgggcattaa gccagcggcg tttcgcggcg tgttgatctg aagtgtcggt
    21601 gtcgtgttta ctcattgctc tccctgattg ctttaatgaa aaagtcatat aagttgccat
    21661 gaacaatgtt tattcatcct ggcgatgata gttatcacca ttattcttta tgggtataaa
    21721 actatcggcg cagcatccta cccgttatcg ataaacgatg caaaacatcc ccttacaatc
    21781 ctgaagggga ataatacaac tgacgaaaaa atgacaaatc tttttgctgg ttaacctgtg
    21841 tactgtccta cacttaatct ttaaaagatt gtgagggcat atgattaagg tgtatggcgt
    21901 acccggatgg ggctctacaa tcagtgagct gatgctaaca ctggctgata ttccttatca
    21961 gttcgttgat gtcagtggtt ttgaccacga gggagcttca cgcgagttat tgaaaaccct
    22021 gaacccgttg tgtcaggttc cgacactggc gctggaaaat gacgagatta tgacggagac
    22081 agcggcgatt gcatttatgg ttctcgatcg tcgcccggac cttgcgccac cggttgggcg
    22141 cgccgaacgt cagcagtttc aacggctatt ggtctggctg gttgctaatg tctatccaac
    22201 attcactttc gccgattacc ctgagcgttg ggcatctgac gcacctgaac agttaaagaa
    22261 gaatgtaatt gaaatctggc tgaattcaca attgaccgct gaaccttatg cctttggtga
    22321 gcaactaacg ctggtggact gttatctctg caccatgcgc acatgggggc cggggcatga
    22381 atggtttcag gataatgcgc agaatatcag cgcgatcgct gatgctgtat gtcagttacc
    22441 gaaattacaa gaagtattaa aaaggaatga aattatttga gtgggatacc agaagattat
    22501 gttatttatt attca
//
LOCUS       DAJOSY010000073        22082 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJOSY010000073 DAJOSY010000000
VERSION     DAJOSY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.073"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..561)
                     /gene="paaY"
                     /locus_tag="OHN27_002931"
     CDS             complement(<1..561)
                     /gene="paaY"
                     /locus_tag="OHN27_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HCQ2287329.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVGEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLV"
     gene            complement(543..1493)
                     /gene="paaX"
                     /locus_tag="OHN27_002932"
     CDS             complement(543..1493)
                     /gene="paaX"
                     /locus_tag="OHN27_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HCQ2287330.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKETAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR"
     gene            complement(1594..2907)
                     /gene="paaF"
                     /locus_tag="OHN27_002933"
     CDS             complement(1594..2907)
                     /gene="paaF"
                     /locus_tag="OHN27_002933"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HCQ2287331.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     NAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(2934..4139)
                     /gene="paaJ"
                     /locus_tag="OHN27_002934"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(2934..4139)
                     /gene="paaJ"
                     /locus_tag="OHN27_002934"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HCQ2287332.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRSAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(4139..4561)
                     /gene="paaI"
                     /locus_tag="OHN27_002935"
     CDS             complement(4139..4561)
                     /gene="paaI"
                     /locus_tag="OHN27_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HCQ2287333.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(4551..5978)
                     /gene="paaC"
                     /locus_tag="OHN27_002936"
     CDS             complement(4551..5978)
                     /gene="paaC"
                     /locus_tag="OHN27_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HCQ2287334.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FEQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVIDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(5980..6768)
                     /gene="paaB"
                     /locus_tag="OHN27_002937"
     CDS             complement(5980..6768)
                     /gene="paaB"
                     /locus_tag="OHN27_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HCQ2287335.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGGDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLAIQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(6768..7535)
                     /locus_tag="OHN27_002938"
     CDS             complement(6768..7535)
                     /locus_tag="OHN27_002938"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HCQ2287336.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(7532..8602)
                     /gene="paaK"
                     /locus_tag="OHN27_002939"
     CDS             complement(7532..8602)
                     /gene="paaK"
                     /locus_tag="OHN27_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HCQ2287337.1"
                     /translation="MTTFHSLTVAKVESETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDDAETALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(8610..9107)
                     /gene="paaD"
                     /locus_tag="OHN27_002940"
     CDS             complement(8610..9107)
                     /gene="paaD"
                     /locus_tag="OHN27_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HCQ2287338.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTNGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLREYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(9122..9868)
                     /gene="paaC"
                     /locus_tag="OHN27_002941"
     CDS             complement(9122..9868)
                     /gene="paaC"
                     /locus_tag="OHN27_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HCQ2287339.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(9877..10164)
                     /gene="paaB"
                     /locus_tag="OHN27_002942"
     CDS             complement(9877..10164)
                     /gene="paaB"
                     /locus_tag="OHN27_002942"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HCQ2287340.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(10176..11105)
                     /gene="paaA"
                     /locus_tag="OHN27_002943"
     CDS             complement(10176..11105)
                     /gene="paaA"
                     /locus_tag="OHN27_002943"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HCQ2287341.1"
                     /translation="MTQEERFEQRVAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLTWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQHARKVA"
     gene            11390..13435
                     /gene="paaZ"
                     /locus_tag="OHN27_002944"
     CDS             11390..13435
                     /gene="paaZ"
                     /locus_tag="OHN27_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HCQ2287342.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHLLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            13683..15956
                     /gene="tynA"
                     /locus_tag="OHN27_002945"
     CDS             13683..15956
                     /gene="tynA"
                     /locus_tag="OHN27_002945"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HCQ2287343.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTPGALKKDK"
     gene            complement(16014..17513)
                     /gene="feaB"
                     /locus_tag="OHN27_002946"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(16014..17513)
                     /gene="feaB"
                     /locus_tag="OHN27_002946"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HCQ2287344.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADRLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVRSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            17749..18654
                     /gene="feaR"
                     /locus_tag="OHN27_002947"
     CDS             17749..18654
                     /gene="feaR"
                     /locus_tag="OHN27_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HCQ2287345.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(18826..19152)
                     /locus_tag="OHN27_002948"
     CDS             complement(18826..19152)
                     /locus_tag="OHN27_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCQ2287346.1"
                     /translation="MKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVADINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGING
                     KWVRKF"
     gene            complement(19160..19345)
                     /locus_tag="OHN27_002949"
     CDS             complement(19160..19345)
                     /locus_tag="OHN27_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCQ2287347.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(19342..21981)
                     /locus_tag="OHN27_002950"
     CDS             complement(19342..21981)
                     /locus_tag="OHN27_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCQ2287348.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT         5308 a         6042 c         5386 g         5346 t
ORIGIN      
        1 taccaggcgt ttcctgccag gttcaacttc ccgcaatggc tcgacttgat gcaacgtttg
       61 cttacagcgt gtcaccagca cctgatactc atgcgtaccc tgctttttcc atgccagctc
      121 ctgctcacta agttcacgaa tcgctttcgc cgggctgccg acaatcagat aattagctgg
      181 catttctgct ttggctttca caaatgcgga tgcaccaaca atgctgttct cgccaatcac
      241 cgcaccgtcc atcactaccg cgttcattcc cactaatgca ttgcggcgga taatgcagcc
      301 gtgaaggata gcgctatgac caatatgtcc atcttctcct acaacagtat cctgctcggg
      361 aaaaccgtgc ataacgcaat tatcctgaat gttcgcgcca tctttcacca cgatacgacc
      421 aaaatcgcca cgcaggctgg cattaggccc aacgtaaacg cccttgccga gaataacatc
      481 gccgatcaat accgctgtcg gatggacaaa gctctcttct ggcacaaccg gagtcagacc
      541 atctatctgg taaattggca taacgcctcc tgttcaatat tcaagccgcc aaaacgttga
      601 aaatacaggc ttcccggcgc aggcagttca ccgactgagg tttcaccttt ttcactaacg
      661 aacgctaaag cagcaggcgc tacgcgctga taaatgttga tacacagctg acgcgccgta
      721 tgccctgccc agtgagccgg aagcaactcc tccggcaaca atgggtcttt aaggacgaca
      781 cggcgataaa aatggatcag taaaagctga atatgaaatg cacgctccgg ggttaactcg
      841 tctgccgtct cttttaacag cggcaccagc gggcggaatg actgaataaa ggtttcgtac
      901 atggcatttt gttcagttaa atgccagcac tcttctactc tggcacgcag tgctgcgcga
      961 gaaagcgcca gtggtatttg cgcttcaaaa caaatcacgt tatccgccac ccccgcttca
     1021 tgcaaaagtg tctgtacatc cgccagtttt tgcgacggcg atgccatcag gctgggtgcc
     1081 agtgcgccaa aaccttgcca gatcaactgc tttttgacat cagccagcgt cgctttatct
     1141 aaaccttccg agagcaacag gagccattta ccatcccatg caggttgctc tgcgcgataa
     1201 attttacttt ctgcccgtcg cgtcaggcgc aagcctttat cactgaggct atagaaactg
     1261 cgtcgcccga tgcgggaaac atccagccag ccttctttat taagacgaaa caaagcggtg
     1321 cgcacgaaac gctcaccaaa tcccagccct tccagcaaag cagccagact accaagccag
     1381 atttcaccac cacgatggga aagcgaatca ccatacagag aggagatcaa agatgtgcca
     1441 ctgaccggaa cagcgtttac agcatgttgg ataaaagtat caagtttact cattcgattc
     1501 agtctgttgt tattgttgtg atttatgaat catagcacag gtcgtatcga cttctggccc
     1561 tgacgcttta cctcaccagg gccagacgac acgtcaggca ccaacaatat tgcgcagatc
     1621 aaacacccga cacgccttgc cttctgaacg cgggatactg ccacagttaa cgatcatcac
     1681 atcggtagag atccccacca tcgacttaat ccgatggcgc agctggtggc atacctggca
     1741 acgctgctca tgcgtcaatg tcaggctact ttctttcaac tccactttca cagaaagtga
     1801 atcaagatgc ccccggcggt tcacctccag ttggtaatgc ggcgataaat gttcgaattt
     1861 gacaatctct tcttccagtt gtgacggaaa gacatttaca ccgcgaataa tcaacatgtc
     1921 atcgctgcgt ccgctgatgc gatccatgcg gcgcatagtc cgcgccgttc ctggtaacag
     1981 tcgggttaaa tcacgcgtgc ggtaacgaat gaccggcaat gcttctttgg tcagcgtggt
     2041 gaataacagt tcgccatgtt caccatcggc aagcggtgtg ccgtcatgag gattaacaat
     2101 ttcaggatag aaatgatctt cccaaatggt tgggccgtcg gtagtttcca gacactccat
     2161 cgccaccccc ggccccatca cttctgacag gccataaata tccagtgcgg tgatccccag
     2221 gcgacgctca atttctttac gcatggcctg tgtccacggc tcagcaccaa acactccaac
     2281 ccgcagcgag caaccgctgg catcaccgcc caactgccgc tccagctctt caatcaggtt
     2341 aaggcaataa gatggcgtaa ccatgatcat atctggttga aaatcacgga tcagttgcgc
     2401 ttgtttttct gtctgcccgc cagacatcgg gatcaccgta gcgcccagac gttcggcacc
     2461 atagtgcgca cccagcccac cagtaaacag cccgtaacca taggcgacat gaattttgtc
     2521 tttgggcgtg ccacccgccg cacgcaacga acgcgctaca atattggccc agttatcaat
     2581 atcgttttgc gtatagccga caactgtcgg tttacctgtg gttcccgaag aagcatgaat
     2641 gcgcaccact tgttccatcg gcacggcaaa ggtgtcgaaa ggatagttgt cgcgcagatc
     2701 ctgtttggtg gtacagggaa atttacgcag gtctgaaagt tccctgaaat catcaggatg
     2761 tacgcctgct gcgttgaatt tgcgccgata catcgggaca ttttcatacg cgtgtttgag
     2821 cgtccatttc agacgctgtg tttgtaacgc ctgtaactca tcaacagacg cggtttcaat
     2881 cgggtcaagc tttgtattgg ttatcattgt agggtactcg caggttgata tgctcaaaca
     2941 cgctccagaa tcatggcgat gccctgaccg acaccgatgc acatggtgca caatgcgtaa
     3001 cgaccgttac gccgatgcag ctcatggctg gcagccagtg ccaggcgggc accactcatt
     3061 cccaacggat ggcctaaggc gatagcgcct ccgttggggt taacatgtgg ggcatcatca
     3121 ggcagcccca attcgcgtag tacacccaac gcctgggccg cgaatgcttc gttcagttca
     3181 atcacgtcca tatcgtgaat actcagcccc gcgcgttcca gcacccggcg agttgcaggc
     3241 accggaccaa gccccatcag gcgcggttcc accccggcgg ttgccatggc tacgatacgc
     3301 gctcgcggtg tcagtccttg cgctgctgcc atctgttcac tggcaataat caacgcagcg
     3361 gctccgtcat tcaccccgga ggcattgcct gcggtgatca ctccattggc acgaaatggt
     3421 gcttttagtc cacgtaactg ttccagcgtc gtttccgggc gcaaatgctc gtcatgttgt
     3481 atttctgtta caacaccttt cttatttttc aacacaaccg gaacaatctc ttcagctaaa
     3541 atgcctgaag attgcgcttt tgccgaacgt tgctgactgc gtagcgcaaa actatcttga
     3601 tcttctcgcg agatttttaa cagttcagct acattctctg ccgtttccgg catgctgtca
     3661 gttccaaatt gttgagccat gagcgggttc acaaatcgcc agccaatagt ggtatcaaac
     3721 atctcagcct gacgagaaaa tgcactggtt gccttgccca taacaaacgg tgcccgagac
     3781 attgactcca cgccaccggc gatcagcaaa tcgccatcgc ccgctttaat cgcccgtgcg
     3841 gcgaacccca gtgcgtccag cccggaacca cacaagcggt taatggttgt gccggaaaca
     3901 ctctgcggca gccccgccag taaagtcgcc atccgggcta cgttacggtt atcttctccc
     3961 gcctgattag cacagccgag gatcacatca tcgatacact ccgcatcgag acgcgggttt
     4021 cgcaccagca gctcccgcaa agggatagca gccagatcgt cagcccgaac acctgataat
     4081 gccccgccgt agcgaccaat tggcgtacga attccgtcac agataaaggc ttcacgcatc
     4141 aggcttctcc tgtaatggtg ccgccgatgc ggtgagattt accgcgaaac agcgcaaccg
     4201 ttttttgttg ttggttaaca atttcgatgt cgtaaacacc ggtttgcttg ccctgatgac
     4261 gtacctgcgc agtagcagtt aaggtgtctc cggcaaagcc tggacgtaaa aaatcaatcg
     4321 tgcaggcaga agcgacggct gccagcccct ggctattgca ggcgtaggca aaggcagtat
     4381 cagccagtga aaatagctgc ccgccgtggc aactttgatg accgttaagc atttgtgcag
     4441 tgacggtcat ggtcactaca gcaaagcctt catccattga gataatgtcg atgccaagcg
     4501 ctttggcgca ggcatcgttc tcatacattg catgggcatt ttgccaggcc ttatgactca
     4561 taaccgctct ccagaagcgc ccgttggcgc agcaatgaac atgggcgata gcgttcttcg
     4621 ccatagtgat gctgtagatt ttcaaggagc cttaatattc gctgccagcc aagttgcgct
     4681 ccccaggcaa gtgggccata tggataattc accccaagac gcatggcggt atcgatatcc
     4741 tgttcagagg ccacgccttt ttgaagcgca tcaagggctt cattgatgat cattgctacc
     4801 gttcgccaaa tcagcattcc tgggtaatct gcaatttgca gcactgtttt gccctgctgt
     4861 tgcaggtaat aaatggcctt gcgggtcgct gagtccgggt tcaccgctgc agcagcaatg
     4921 gtcaccacct tgcccgccat tttatcgatc actaccaccg ggcgtgccag tcgtattgcc
     4981 agcgcctgtg ccgtctcgcc ttgtgtctca atcaataaaa catcgtcaat ttccgtgaca
     5041 ccgtcacttt tcttttctac tttcattggg ctaaaactgt cgcttaccgc ttccaggcca
     5101 acaactgcct cacgttccgc gcgccagtcg tacacgccca gcccactttt cttgcccaac
     5161 cgtccaccaa tcaccagttc ctgttgcacc agcgaaggta aaaaacgacg ctcctgccag
     5221 aaagcgttaa acaccgaaca ggtgacagca aaattgacgt cctgaccaat cagatcggtt
     5281 aattccagcg gccccatcgg gaaaccagct ccatcgcgaa gtgcagcgtc aatcacttct
     5341 ggtgcagcaa cctgctcttc cagtgcccgc caggcctcgg aataataagg acgcgcaaca
     5401 cggttaacga taaatccggg ggtcgaatga cagcgcactg gctgcttacc ccaactcaat
     5461 gtcagttcac acaactgctc gacaacttcc gctgccgttg ccagcccact gaccacctcc
     5521 accaacttca tcaccggtgc cgggttaaaa aaatgcagcc ccgcaacacg ttcaggattt
     5581 tttacctccg cagcaatcgc ggttatagag attgacgaag tgttagtggt caatagcgtt
     5641 tgtggcgggc aaacttccgc cagttgttca aagagcgctt ttttaacttc cagacgttca
     5701 gacgccgctt caataaccag attcgctgct gccagcgcgt gaatatcggt caccgggatc
     5761 aggcgtttca atgtgcgttc acaggtttca gcggtcagtt ttccccgcgt cacgcgtgaa
     5821 tttagccgcg cgtgtatccc gtcgattgcg cgggtcagcg cttcagcaga aatgtcatac
     5881 agtaaaacct gatgtccatg actggcggca acttcagcaa tgcctgcccc catggtgccg
     5941 ctcccaatca ctgccacagt ttgcacattt atcatcatgc tatttccccg tgaactgcgg
     6001 tgagcgttta gccaggaacg cactgacacc ttcacgataa tctgcgctcc gtccggcaag
     6061 tcgctgatag tcacgttcca gatccagttg cgtatcgagc gtattggttt cagcgctatt
     6121 tatcgcttgc ttgataagtc caagaccaaa tgtcggttgt atcgccagat gccgtgccag
     6181 ctgttgcgcg gtatctgcca gcgtttcatc atcaacaacc tgccagatca tcccccattc
     6241 gtgcgcctgt tcagcactca gttgattccc cagcagtgcc agccccatgg cgcgcgctcg
     6301 tccggcaacg cgtggcagta accaggttcc accgcaatcg ggtattaagc ctaacttact
     6361 aaacgccatg acgaattttg ctgaacgggc agcaataacg atgtcgcccc ccagtgccag
     6421 tgttgcgcct gcccctgccg ccacgccatt gactgcacag atcaccggtt ttggcagttt
     6481 tgccaggcga cgtaccagtg ggttatagaa acgttcaact gacatcccta aatccggtgc
     6541 ggggcctgtg ggatcaacgt tacgatcgtt aagatcctga ccagcacaaa atccgcgccc
     6601 ggcacctgta agtaacaggc aacggatagt gtcgtcgcgc tcgacctgtt tcaggcactc
     6661 tgccagttgt gcgtgcatct catcattaaa actgttcagg cgttccgggc ggttgagcgt
     6721 tagtgtcatc acgccctttt ctacatgact gaggatgaat tccatcatta gcgtccttta
     6781 aagtcgggcg tgcgtttttg taagaaagcg gagatgcctt catgacgatc ttctgttgcc
     6841 gccagcaagg tgaataactg tcgctcctgg gcaagtccgg cttgcaaagc cacttcctgc
     6901 gactggcgca gcgcttgctt tgccgcttgt aaggccagcg gcgagtgacg tgccattttc
     6961 gatgccagct gtaaggcgta ttcgagggtt aaatcgctgg ggaagacgtc gctaaccagc
     7021 ccggcctgct gtgcttgctg agcggtgata ctttctccgc tcagcaccat tttgctggct
     7081 aacgatttac cgacactacg gattaaacgt tgcgttccgc ctgcgccagg catgatgccg
     7141 agagtgattt ccggcaaccc aaaacgcgcg ttctctccgg caaccaccac atcgcacaac
     7201 aatgccagtt cgcaacccgc cccaagcgcg taaccattga cggctgcgat aagtggttta
     7261 ttaaaggcct gcaatcgcgc ccatagctgc ggacgtgtat cgtttaaggt ggccgcgaga
     7321 tctttttctg ccatttcgtt gagatcggcc ccagcggcaa aaaagcgtgc attaccggta
     7381 atcacacaga ccgaaatgct ggtatcggta gccgcagctt ccagttcatt taccagttgc
     7441 atcagcaggg cattatttag cgcattacgt gcggcgggac ggttaagggt cagcaacaat
     7501 actcgttgct gacggctgac gatcagttcg ctcatgccat ccccttcgcg tcaaagtcaa
     7561 ccaccacatc gctggtcagc ggcagtgcct ggcaactcaa cacataacct gcggccagtt
     7621 catccggttc cagactgtaa ttggtttcca tcgccacttt gccacgcagc actttgcatt
     7681 tgcaggtcgc acagacgccg cctttgcagg catagggcag atccgccccc tggcgcaatg
     7741 ccgcatcgag aatgctttca tcgtcggcat taagcacgat ttcccgatcc cgcccatcct
     7801 gacgtacagt cactttttgt ccgtcacttt gcacgttaac gctacgtttg acgcgcgtgc
     7861 caggcgtatt aaaccgctcc agatgaatgg ttttatctgg cattcccagt gcttttaagg
     7921 cggtttccgc gtcatccatc atcgccgccg gaccacaaat aaatgcctca tcataaagac
     7981 gaaaattaat gagcgaggcc ccaagtgact gtaatttttc accgtcaata cgcccgtgaa
     8041 gcagatcgct gtcgagggtt tcctgactga aaatgcacaa caactgtaaa cgctgaggat
     8101 atttgtcttt caggtctgcc agtgcctggc gaaacatcat gctctggctg gtacggttac
     8161 cgtagatcag ggtgaactga ctttcaggct cggtttgtaa agtggtggcg ataatcgcca
     8221 gcattggcgt aatacctgat cctgctgcaa ttgccagata gcgcccctgg cgttcggcct
     8281 gcggctgata gccgaaatgc ccctgcggca ccatgacctc cagcgtcata ccctggcgga
     8341 tgtgttcgcg ggcatagcgg gagaaacgtc cgccttcaat ggctttcacc gccacactaa
     8401 tttcgccagg cagatagctg cggcaaatgg agtaacaacg gcgtaattct tcaccatcaa
     8461 agctggcttt taaggtcaaa tgttgaccgg ggcgaaagcg atacgcctcc tgcaaaggct
     8521 ggggcaccgc aaaggtaatg gtcaccgcat cacgggtttc cgactcgact tttgccactg
     8581 ttaaggaatg aaacgttgtc atggcatcct caaatacatt tgaaataatc gaaaggttcg
     8641 cggcaactat cgcagcggta caatgctttg caggccgtgg aaccaaattc actgataagt
     8701 gtggtatgga cgctggcgca gcgcgggcaa cgtacttctg gcggcaaatg ggcatggcaa
     8761 ctgtgtccgg cgggcgggct aatgccatac tctcgcagac gctcacgggc atcgggggtc
     8821 atccagtcgg tggtccatgc cgggtcgagt tgcagcacaa cctgaacggg ggtaaacccg
     8881 ttggttgtca ttgcctcacg tatcgcgcca atcaaatgtt ccgttgccgg acaaccggaa
     8941 tatgtcggcg taaagccgat cacccatcct tctcccatct gtgtcacatt acgcaccatg
     9001 cctaaatcag taatggtcag caccgggatc tccgggtccg ggatctggct gagtagcgcc
     9061 catatctcat gaacctgtgg cggtgcaatg gtggccagac gttgcatacc catctcctct
     9121 gttaccattg ctgaccgggc aagacacgct ggagatactg catttctgcc agcatgggtc
     9181 caaggtgttc ggtatgcagt ccttttttac cgccagtgcg atacgcctgc tcttgcggta
     9241 cgttcaatgt ggcttcgttg atcccggcaa aaacttcggc ttcccacgct gcgcgtaaag
     9301 tgcgtggatc aaccgcaata ccctcttcac tcagtgcaat atcaatctcg tcggcatcga
     9361 acagttcggc ggtaaaacgc cacaacttgt tgatggcctg ctgcatcttt tgccctgata
     9421 cgtcagtacc attgcccagt cgctccagcc agccacgact aaaacgcagg tgatagcgcg
     9481 cttctttaat tgctttggca gaaatcgccg ccagttgcgg atcacggctt tccatcagac
     9541 gggtaaagag cgccacatgc catgcatcga tgaaatactg gcgtgcaatg gtgtcggcaa
     9601 aattgccgtt tggttgttca accaacaata agttgctgaa ctggcgctcg tctcgggtga
     9661 aggccagggt atcttcatcg ccttctcccg ctaattcagc ggcatacgat aagaagttgc
     9721 gtgcctgacc taataaatcg aggccaatgt ttgccagtgc gagatcgatt tccagttccg
     9781 gtgcgtgacc gcaccattca cccaaccgct gggagagcac caggcagtta tcgcccaggc
     9841 gcaaggtgta agccgttaac tgattcattt ccgacctcac atgtgctcaa tgccatcagg
     9901 gatggtgtaa aacgttggat ggcgatagac cttgctttca gccggatcaa aaaattcacc
     9961 gcgttcttcc ggttgcgagg caacaatttc actcgccttc accacccaaa ttgaacatcc
    10021 ttcgctacga cgggtgtaag catcacgggc attttccagt gccatccgct catcggcagc
    10081 atgtaaactg ccaacatggc ggtgtgataa gccctgtttg ccacgcacga aaacttcgta
    10141 taacggccag taaacattac tcattttgaa tctccttatg cgaccttacg ggcatgttgt
    10201 ttttgtgcat gggccagcgc tgcttcccgt acccaggtac cttcttccca ggctttacgt
    10261 ttggcgtcga gcctctcctg attacaaatt ccgcgaccgt taattacttc gttaaactcc
    10321 tgccagtcga tctcaccaaa gcggtagtga ccgctttcag tgtcaaaatg cagatccggg
    10381 tcaggaacgg tcataccgag catttcaacc tgtggaacgg tgttatccac gaaacgctgg
    10441 cggagttcgt cgttggtgaa acgtttgatt ttccaggtga gacttctggc gctgtttggc
    10501 gagttatcat cgtttggccc gaacatcatt aaggctggcc accagaaacg gttaatggcg
    10561 tcttgcaaca tctgcttttg agcttcgctg ccctgcgcca gtgccatgca ggcttcaaac
    10621 ccctgacgct ggtgaaaact ctcttctttg cagattttca ccattgctct ggcatacggg
    10681 ccataagaag tgcggcataa cgccacctga ttgacgatcg ctgcgccatc caccagccag
    10741 ccgataacac cgatatcagc ccaactcaat gtcggataat taaagatgga ggaatatttc
    10801 atccgtccgt cgagcatttt ttggtagatg tcttctctgg cgcagcccag tgtttcggcg
    10861 gcgctataga gatacagacc atgaccggct tcatcctgca ctttggccaa cagaatggct
    10921 ttacgccgca aggttggtgc gcgagtgatc cagttacctt caggcaacat accaacaatc
    10981 tcggagtgcg catgctgccc aatctggcgg atcaatgtct ttcggtaagc atcgggcatc
    11041 cagtcctgtg gctcgatagc cgtttcctgg gctacccgtt gctcaaagcg ttcttcttgg
    11101 gtcacgtcat cacctttacg attccagttt tcgtgaatta attaaaaaca cgaatcacat
    11161 tgttttatga aagttacaca atagttaagt aaaatcacaa attttgtatg tttgaactgt
    11221 gacggatttc gcaccctatt tttataaatc attatttatc agtgcattaa tgttttccgc
    11281 agttgctata cagatcgcat agttaacatt tcgttaaaag atcccttgct ttttatgatt
    11341 cgcgatttaa ctattagcaa cagaaatgtg aaacatctgg agagtagcga tgcagcagtt
    11401 agccagtttc ttatccggta cctggcagtc tggccggggc cgtagccgtt tgattcacca
    11461 cgctattagc ggcgaggcgt tatgggaagt gaccagtgaa ggtcttgata tggcggctgc
    11521 ccgccagttt gccattgaaa aaggtgcccc cgcccttcgc gctatgacct ttatcgaacg
    11581 tgcggcgatg cttaaagcgg tcgctaaaca tctgctgagt gaaaaagagc gtttctatgc
    11641 tctttctgcg caaacaggcg caacgcgggc agacagttgg gttgatattg aaggtggcat
    11701 tgggacgtta tttacttacg ccagcctcgg tagccgggag ctgcctgacg atacgctgtg
    11761 gccggaagat gaattgatcc ccttatcgaa agaaggtgga tttgccgcgc gccatttact
    11821 gacctcaaag tcaggcgtgg cagtgcatat taacgccttt aacttcccct gctggggaat
    11881 gctggaaaag ctggcaccaa cgtggctggg cggaatgcca gccatcatca aaccagctac
    11941 cgcgacggcc caactgactc aggcgatggt gaaatcaatt gtcgatagtg gtcttgttcc
    12001 cgaaggcgca attagtctga tctgcggtag tgctggcgac ttgttggatc atctggacag
    12061 ccaggatgtg gtgactttca cggggtcagc ggcgaccgga cagatgctgc gagttcagcc
    12121 aaatatcgtc gccaaatcta tccccttcac tatggaagct gattccctga actgctgcgt
    12181 actgggcgaa gatgtcaccc cggatcaacc ggagtttgcg ctgtttattc gtgaagttgt
    12241 gcgtgagatg accacaaaag ccgggcaaaa atgtacggca atccggcgga ttattgtgcc
    12301 gcaggcattg gttaatgctg tcagtgatgc tctggttgcg cgattacaga aagtcgtggt
    12361 cggtgatcct gctcaggaag gcgtgaaaat gggcgcactg gtaaatgctg agcagcgtgc
    12421 cgatgtgcag gaaaaagtga acatattgct ggctgcagga tgcgagattc gcctcggtgg
    12481 tcaggcggat ttatctgctg cgggtgcctt cttcccgcca accttattgt actgtccgca
    12541 gccggatgaa acaccggcgg tacatgcaac agaagccttt ggccctgtcg caacgctgat
    12601 gccagcacaa aaccagcgac atgctctgca actggcttgt gcaggcggcg gtagccttgc
    12661 gggaacgctg gtgacggctg atccgcaaat tgcgcgtcag tttattgccg acgcggcacg
    12721 tacgcatggg cgaattcaga tcctcaatga agagtcggca aaagaatcca ccgggcatgg
    12781 ctccccactg ccacaactgg tacatggtgg gcctggtcgc gcaggaggcg gtgaagaatt
    12841 aggcggttta cgagcggtga aacattacat gcagcgaacc gctgttcagg gtagtccgac
    12901 gatgcttgcc gctatcagta aacagtgggt gcgcggtgcg aaagtcgaag aagatcgtat
    12961 tcatccgttc cgcaaatatt ttgaggagct acaaccaggc gacagcctgt tgactccccg
    13021 ccgcacaatg acagaggccg atattgttaa ctttgcttgc ctcagcggcg atcatttcta
    13081 tgcacatatg gataagattg ctgctgccga atctattttc ggtgagcggg tggtgcatgg
    13141 gtattttgtg ctttctgcgg ctgcgggtct gtttgtcgat gccggtgtcg gtccggtcat
    13201 tgctaactac gggctggaaa gcttgcgttt tatcgaaccc gtaaagccag gcgataccat
    13261 ccaggtgcgt ctcacctgta agcgcaagac gctgaaaaaa cagcgtagcg cagaagaaaa
    13321 accaacaggt gtggtggaat gggctgtaga ggtattcaat cagcatcaaa ccccggtggc
    13381 gctgtattca attctgacgc tggtggccag gcagcacggt gattttgtcg attaatcggt
    13441 gaatgaaggg caacggcgaa tagttgccct tttatttcac taagttttgt gacgttgtca
    13501 cattatgcat gatgtgtaca tctattttca gggcatccac tgtatgaaaa gctggcacac
    13561 ctgccaaaca acctggcagg tgcaggcaat cccctttgca tcagtactga taatgtgaac
    13621 ctgactaaac cgcccacaga gcgcggttgc taacaagaac acaacatctg acgaggttaa
    13681 taatgggaag cccctctctg tattctgccc gtaaaacaac cctggcgttg gcagtcgcct
    13741 taagtttcgc ctggcaagcg ccggtatttg cccacggtgg tgaagcgcat atggtgccaa
    13801 tggataaaac gcttaaagaa tttggtgccg atgtgcagtg ggacgactac gcccagctct
    13861 ttaccctgat taaagatggc gcgtacgtga aagtgaagcc tggtgcgcaa acagcaattg
    13921 ttaatggtca gcctctggca ctgcaagtac cggtagtgat gaaagacaat aaagcctggg
    13981 tttctgacac ctttattaac gatgttttcc agtccgggct ggatcaaacc tttcaggtag
    14041 aaaagcgccc tcacccactt aatgcgctaa ctgcggacga aattaaacag gccgttgaaa
    14101 ttgttaaagc ttccgcggac ttcaaaccca atacccgttt tactgagatc tccctgctac
    14161 cgccagataa agaagctgtc tgggcgtttg cgctggaaaa caaaccggtt gaccagccgc
    14221 gcaaagccga cgtcattatg ctcgacggca aacatatcat cgaagcggtg gtggatctgc
    14281 aaaacaacaa actgctctcc tggcaaccca ttaaagacgc ccacggtatg gtgttgctgg
    14341 atgatttcgc cagtgtgcag aacattatta acaacagtga agaatttgcc gctgccgtga
    14401 agaaacgcgg tattactgat gcgaaaaaag tgattaccac gccgctgacc gtaggttatt
    14461 tcgatggtaa agatggcctg aaacaagatg cccggttgct caaagtcatc agctatcttg
    14521 atgtcggtga tggcaactac tgggcacatc ccatcgaaaa cctggtggcg gtcgttgatt
    14581 tagaacagaa aaaaatcgtt aagattgaag aaggtccggt agttccggta ccaatgaccg
    14641 cacgcccatt tgatggccgt gaccgcgttg ctccggcagt taagcctatg caaatcattg
    14701 agcctgaagg taaaaattac accattactg gcgatatgat tcactggcgg aactgggatt
    14761 ttcacctcag catgaactct cgcgtcgggc cgatgatctc caccgtgact tataacgaca
    14821 atggcaccaa acgcaaagtc atgtacgaag gttctctcgg cggcatgatt gtgccttacg
    14881 gtgatcctga tattggctgg tactttaaag cgtatctgga ctctggtgac tacggtatgg
    14941 gcacgctaac ctcaccaatt gctcgtggta aagatgcccc gtctaacgca gtgctcctta
    15001 atgaaaccat cgccgactac actggcgtgc cgatggagat ccctcgcgct atcgcggtat
    15061 ttgaacgtta tgccgggccg gagtataagc atcaggaaat gggccagccc aacgtcagta
    15121 ccgaacgccg ggagttagtg gtgcgctgga tcagtacagt gggtaactat gactacattt
    15181 ttgactggat cttccatgaa aacggcacta ttggcatcga tgccggtgct acgggcatcg
    15241 aagcggtgaa aggtgttaaa gcgaaaacca tgcacgatga gacggcgaaa gatgacacgc
    15301 gctacggcac gcttatcgat cacaatatcg tgggtactac acaccaacat atttataatt
    15361 tccgcctcga tctggatgta gatggcgaga ataacagcct ggtggcgatg gacccagtgg
    15421 taaaaccgaa tactgccggt ggcccacgca ccagtaccat gcaagttaat cagtacaaca
    15481 tcggcaatga acaggatgcc gcacagaaat ttgatccggg cacgattcgt ctgttgagta
    15541 acccgaacaa agagaaccgc atgggcaatc cggtttccta tcaaattatt ccttatgcag
    15601 gtggtactca cccggtagca aaaggtgccc agttcgcgcc ggacgagtgg atctatcatc
    15661 gtttaagctt tatggacaag cagctctggg taacgcgtta tcatcctggc gagcgtttcc
    15721 cggaaggcaa atatccgaac cgttctactc atgacaccgg tcttggacaa tacagtaagg
    15781 ataacgagtc gctggacaac accgacgccg ttgtctggat gaccaccggc accacacatg
    15841 tggcccgcgc cgaagagtgg ccgattatgc cgaccgaatg ggtacatact ctgctgaaac
    15901 catggaactt ctttgacgaa acgccaacgc caggggcgct gaagaaagat aagtgattgt
    15961 ttcagacaaa aaaacggcac caggtgccgt tttttactta tgagcgaacc agattaatac
    16021 cgtacacaca ccgacttagt ttcacaccaa ccgtccagcc agtcggggcc aaaatcacgg
    16081 cccgttcctg actgcttcat cccaccaaac ggtaagttag cgtcaattaa ggtatggctg
    16141 tttacccaca ccgtccctgc ctgtaagcga tcgctatatt ccagagcctg ggagagattt
    16201 tgcgtccaga cactggcagt taagccatat tccgtgtcgt ttgccagttg taacgcctct
    16261 tctccatccg ctactcgcac caggtttacc accggaccaa acacctcttc acgagttaaa
    16321 cgcaatttag catcgggatt taccaccagc gttggcgcaa cataataccc ctctccggct
    16381 ggtccattcg acccgcgaat cagctctgct tgctgtgcct gcgcatcgtc gaggaatgaa
    16441 cgcactttgt cgcagtgcgc acgagaaacc aaagggttaa tctgtgcaac aggtgacatc
    16501 cccggtccca cttgcaacga ttttaccgcc tgctcaaatc cactaaccag cgtgtcaaac
    16561 aacggcgctt caatataaat tcgcgaactg gcggcgcata cttgcccctg attcaggaaa
    16621 cttccggtca tcaatccttc aataacccat tgcggatcag catcttttaa tacaattgcc
    16681 gggtttttac cgcccagttc cagcgttaca cgcgttaagc gatcagcagc agttctggca
    16741 atacctttgc ccgtcgcggt tgaaccggta aaactgattt tcgcaacatg aggatgtgac
    16801 gtcagggccg cgccgcatac agcacctgac ccggtgacca cattaaaaac gccatcagga
    16861 ataccagcct cgctggccag ttccgccacg cgcaacatcg tcagtggcgt ggtttccgaa
    16921 ggcttaatca cgattgaaca gcctgctgcc agtgcaggca tcaccttcca catgccaatc
    16981 atcaacggaa agttccatgg cacaattccc gctaccacgc caaccggctc tttacgcgtc
    17041 caggcctgat aacgcgcccc ctggggtaag ggaatcgaca agtccagcgt tttacccgcg
    17101 attttggtcg ttaacccggc ggtataacgc atccagttca gcgtacagcc cacttcaaaa
    17161 gcacgggaaa tggcaattga cttgccttgc tccagggttt ccagttgcgc cagctcctca
    17221 ctgtgctgct ccaccagatc agcaaaacgt agcagaatac gttcacgctc tgcgggtaat
    17281 cgcccggccc agcgacgcga gacgaaggcc cgccaggcag acatgactgc gttatctaca
    17341 tccgcttcgt tggcatcagc agtagacgca atttcttgcc cggtggccgg atcaaagatc
    17401 gccaaccgtt tttcactttg tgcggggcca ggacgaccat caatataaag accgtgttga
    17461 cgatcgagaa actgttggac ctggcttaat actgctacat gcggctctgt catcactttt
    17521 ccttattatt tacccagtgt gatttcagtg tacgcagcga caagaaaagt cacttttttc
    17581 gctgtgacat tcgctttgtc agctgtgaca agctccgcaa atcgtgacaa taaccgctta
    17641 tgtctctatg cctgacaaga cttatttcct atagtcaggc tcattagctt cgattatcgc
    17701 aacacaaatg caacaataaa aatacatttc acagagcgaa aacgtgccat gaaccccgca
    17761 gtggataatg agtttcagca atggctttcc caaatcaatc aggtatgcgg aaattttacc
    17821 ggacgcctgc tgactgagcg ttacacgggt gtactggaca cgcattttgc caaaggacta
    17881 aagctgagta ccgtgacaac cagcggggtg aatttatccc gcacctggca ggaagtaaaa
    17941 ggcagcgacg atgcctggtt ttacaccgtt tttcagctta gtggtcaggc aataatggag
    18001 caggatgagc gtcaggtgca gattggcgct ggcgatatta cgttactcga tgcctcacgc
    18061 ccctgttcgc tttactggca ggagtcttct aaacagattt cattactttt gccacgcact
    18121 ctgctggaac aatattttcc ccatcaaaaa cctgtctgcg cagaaagact ggacgctgac
    18181 ttacccatgg tgcaactcag tcatcgcctg ttacaggaga gcatgaataa tccggcactt
    18241 tctgaaacag aaagtgaagc tgcgctacag gcgatggtgt gtctgctgcg cccggtactt
    18301 catcagcggg aatctgttca acctcgtcgt gaacgtcagt ttcaaaaagt ggttacgttg
    18361 atagacgata atattcgcga agagatatta cgcccggagt ggatagccgg agagacaggt
    18421 atgtcagtac gtagtttgta ccgaatgttt gccgataaag gtttggtagt cgcgcaatat
    18481 attcgtaacc gtcgtctcga tttttgtgca gatgcgattc gccatgccgc agatgatgaa
    18541 aaactggcag gcatcggctt tcattgggga ttttctgacc agagtcattt ttcaacggta
    18601 tttaagcaac gctttgggat gacgccaggc gagtatcgac gtaaattccg ctaactaatt
    18661 ggcgttgcat gtactgcaac gccaattagt taaaacagat atttgactat gcatttttca
    18721 gggcaaaagg caatttgaca ggagtttttg aatattcacc ttgtttcagg aaaatagatc
    18781 aatcgccttc tcctgtagcg ttatcccgat attgcggttt tgctattaaa attttcgcac
    18841 ccatttaccg tttatccctt gtacatattc ccccggtttg gcccgagcta cgagcttttg
    18901 cccggcgagt ttcgcgatat catctaccga cacattattt tgctttgcca gttgttggta
    18961 actcgcttta cgttcggcat taatgtcggc tactaatttc tctgtttcag catccgtttt
    19021 cagcgcaacc agataaccgt aaaatgtttc acctacccgc ccctgagttc tggcttcatc
    19081 cagagtcaat gccattacat tgctgcttac cagcccaaca agaaacgcac aaagaagtaa
    19141 tgttttcttc atcatcacct cagaaaagat cgctacgagt ttcaagcagc tcttcgacat
    19201 ctttgtctgc cttgatgatg atctcatgct caattttaac gttcatattg atagtgatcg
    19261 gttccttagg tgcagcgact tcaatgcgag gcgtacagcc aaccagcata aaagatgacg
    19321 tcaacgcagc cagtaaaatt ttcattgttt ttcctcacac tcttttcctt gcggacagtc
    19381 atttcccggc agacgtgcgt tctgctccaa ccatgcctgg agattatcgc caaagcgtaa
    19441 actgcgccac agcgtaaaaa tattctcttc atgatggtaa ttaaggttta ccgtaccgct
    19501 tttaccatca acgcgactgg tacctgtaat gttggcctgc atggttaata aaccgagatt
    19561 atctaaatta atatttgtcg atgaacggct aatttccata tagcgcaacc agttaattgc
    19621 tgaacccgca gtcatattgt ctttcaccac cgcatctgcg gtgtctttat ccagccgcaa
    19681 ggtcattggc ccgctatttg ccagccaacc atctttcact atccattttt cgttgtttag
    19741 ccataacggc aatgcaccac taaatgcccc ggacatggcg aattgtttcg gattgacggc
    19801 gctaaccagt tcgctggatg agagattatt cagccgcaac agcgccgggt catgttgcgg
    19861 catacgtaat tgtttcatca gtacgttacc gcctaacaca tcgacgctaa catcagtcaa
    19921 cagcaagggt tcttcttcag tccacggata acgcccttgc aaatcagccg taatattttt
    19981 cgctgtcacc agattaatca cttcggcaat tcgcaacgta acggggccgc gagtccccag
    20041 atgccaggct ccatcggcaa aacggaaagg caggacaaaa tcgacaccgt taacctgatt
    20101 atctggcatc caggcactac cgcctttcaa cacgccgtgc cctcccgcgc ggaatccttg
    20161 ttcaggcgca gcagaaaatg caacctgagc atacagttca ccatcgcgta agttcatctt
    20221 ccagtcgggt ggcaccagcg gctggaatac agtcagtgat tgttttggcc accaggcgtt
    20281 accgcgcaga cgaataccgt cccagcgacc atttacccga accgggccaa tctcaccagc
    20341 atgtaaatcg cctttaaaga ggaaatacgt cggatcgcgc ccatcgacgc taaattttaa
    20401 ggttgatggc ggtaacacac tgccgccagt gaacagcgtt tgcccggcgt ccagtgacag
    20461 cgcgccgctg aaggagggat gctgtgcgtc acgtacccag acgacgggct tgtcgagaat
    20521 taatcgcggc ttttctaccg tcatcgtacc gtattgtaac tggtcgaaac cggtggagag
    20581 gtcggtcagc gtaatcgtgc tgtcatgcca ttcacctttt cctgcgacat cccaggtggc
    20641 attcatcggt gtaaaactgc cttttcccca gtagcgccac tgccagcggc ccgcgtcagg
    20701 gagaaaatca ttcgccagcc catccatatg cagcacgaaa tcgcccagtt cattttcatg
    20761 cgcctgcaaa atggcctgca aacgtccgtc aacaccacgt tgggtgactt ttacacccgc
    20821 taaaggccag cggatttcat cgatgtccag cgaatcgatg actcttccct tcgaacgtaa
    20881 taacgcgcct ggctcaaagg ccagcgttgg gtcagtcaga cttccactta gctgcgcggg
    20941 taagcgggca tataaaatga ggtccgcctg tttcgcttca ccggtcagct gcagaggcag
    21001 ctggctgtta tccatgctta attttcctgg gccaaaattt agcaccgcgt tgcctttacc
    21061 cgcttgcccc tgggtcagca cactcagtcg tccgctgatc agagcgttct caagccctgc
    21121 ctgccagttg tcgactttga cacccagtcg gccactcaaa ggaaaacctg catacggcca
    21181 actccagcga ccatcgctta cggtcaattg ttgacgagta atttgccacg gcaaatcgag
    21241 caacggatcg ccgttatccc gtgccagcac aatcaattgc ccgctatttt cctgccagtc
    21301 cagctctgca tccaccagtg acggttcctg cggcaagttt aacgtcgcag tagcatgccc
    21361 acttacagga agtccatccg gcacgagcgg catagtaaat tcccccacca gtttaaccgg
    21421 cggctgattt tcaaacgcga cgacatccag ttcgctgact gtaagttgtt gccctttcag
    21481 ctggccttga aatttaactt tttcgccctg ataacgcagt tgctggatat cagaggttaa
    21541 tgcgagagag agttttccct gccattcctg ccagggagaa aaaatcagtt tatcgatatt
    21601 gatccaggtg ttaggcagca tggactgcca ctgcgcgagg gtttttggag cagctggcga
    21661 ttgctccgtc tgcggcaatt tcgccagaca agcagaatca agttctaccg tgccgacgtt
    21721 caataaccag cggctgggat gtgaaaggct ggcgttggtg atatgcgcaa gctgacaatc
    21781 tcccaccaga taacggagat cgggaatgat taaaccttta cgcgtaatgc gtgggctttc
    21841 atctaatgca atacgtgtcc cgagcggtag ccagatgccc gccagcgtgg gaacccacag
    21901 cccgagcgtc atcaacagcg tcaacggcac aagaataatc agtaataaca gcgcgagaac
    21961 ggctttatat ttacccagca tgggtagtta atatcctgat ttagcgaaaa attaagcatt
    22021 caatacgggt attgtggcat gtttaaccgt tcagttgaag gttgcgccta cactaagcat
    22081 ag
//
LOCUS       DAJOSY010000074        21869 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJOSY010000074 DAJOSY010000000
VERSION     DAJOSY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.074"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..432)
                     /locus_tag="OHN27_002951"
     CDS             complement(<1..432)
                     /locus_tag="OHN27_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCQ2287349.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRG"
     gene            complement(635..1036)
                     /gene="nikR"
                     /locus_tag="OHN27_002952"
     CDS             complement(635..1036)
                     /gene="nikR"
                     /locus_tag="OHN27_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCQ2287350.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1042..1848)
                     /gene="nikE"
                     /locus_tag="OHN27_002953"
     CDS             complement(1042..1848)
                     /gene="nikE"
                     /locus_tag="OHN27_002953"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCQ2287351.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGEKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1845..2609)
                     /gene="nikD"
                     /locus_tag="OHN27_002954"
     CDS             complement(1845..2609)
                     /gene="nikD"
                     /locus_tag="OHN27_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCQ2287352.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2609..3442)
                     /gene="nikC"
                     /locus_tag="OHN27_002955"
     CDS             complement(2609..3442)
                     /gene="nikC"
                     /locus_tag="OHN27_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCQ2287353.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3439..4383)
                     /gene="nikB"
                     /locus_tag="OHN27_002956"
     CDS             complement(3439..4383)
                     /gene="nikB"
                     /locus_tag="OHN27_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCQ2287354.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4383..5957)
                     /gene="nikA"
                     /locus_tag="OHN27_002957"
     CDS             complement(4383..5957)
                     /gene="nikA"
                     /locus_tag="OHN27_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCQ2287355.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6068..6655)
                     /gene="acpT"
                     /locus_tag="OHN27_002958"
     CDS             complement(6068..6655)
                     /gene="acpT"
                     /locus_tag="OHN27_002958"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCQ2287356.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6710..7759)
                     /locus_tag="OHN27_002959"
     CDS             complement(6710..7759)
                     /locus_tag="OHN27_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCQ2287357.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILESRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7891..9108
                     /locus_tag="OHN27_002960"
     CDS             7891..9108
                     /locus_tag="OHN27_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2287358.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADSLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9112..9669)
                     /gene="dcrB"
                     /locus_tag="OHN27_002961"
     CDS             complement(9112..9669)
                     /gene="dcrB"
                     /locus_tag="OHN27_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCQ2287359.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9742..10407)
                     /locus_tag="OHN27_002962"
     CDS             complement(9742..10407)
                     /locus_tag="OHN27_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCQ2287360.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10628..10873
                     /gene="tusA"
                     /locus_tag="OHN27_002963"
     CDS             10628..10873
                     /gene="tusA"
                     /locus_tag="OHN27_002963"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCQ2287361.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10975..13173)
                     /gene="zntA"
                     /locus_tag="OHN27_002964"
     CDS             complement(10975..13173)
                     /gene="zntA"
                     /locus_tag="OHN27_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCQ2287362.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNYLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13247..13873)
                     /locus_tag="OHN27_002965"
     CDS             complement(13247..13873)
                     /locus_tag="OHN27_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCQ2287363.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLVGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14014..14373
                     /locus_tag="OHN27_002966"
     CDS             14014..14373
                     /locus_tag="OHN27_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCQ2287364.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14376..14645)
                     /locus_tag="OHN27_002967"
     CDS             complement(14376..14645)
                     /locus_tag="OHN27_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCQ2287365.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14635..15231)
                     /gene="rsmD"
                     /locus_tag="OHN27_002968"
     CDS             complement(14635..15231)
                     /gene="rsmD"
                     /locus_tag="OHN27_002968"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCQ2287366.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAEATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15381..16874
                     /gene="ftsY"
                     /locus_tag="OHN27_002969"
     CDS             15381..16874
                     /gene="ftsY"
                     /locus_tag="OHN27_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCQ2287367.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16877..17545
                     /gene="ftsE"
                     /locus_tag="OHN27_002970"
     CDS             16877..17545
                     /gene="ftsE"
                     /locus_tag="OHN27_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCQ2287368.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17538..18596
                     /gene="ftsX"
                     /locus_tag="OHN27_002971"
     CDS             17538..18596
                     /gene="ftsX"
                     /locus_tag="OHN27_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCQ2287369.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18841..19695
                     /gene="rpoH"
                     /locus_tag="OHN27_002972"
     CDS             18841..19695
                     /gene="rpoH"
                     /locus_tag="OHN27_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCQ2287370.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19966..21069
                     /gene="livJ"
                     /locus_tag="OHN27_002973"
     CDS             19966..21069
                     /gene="livJ"
                     /locus_tag="OHN27_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCQ2287371.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARSYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21268..21651)
                     /gene="panM"
                     /locus_tag="OHN27_002974"
     CDS             complement(21268..21651)
                     /gene="panM"
                     /locus_tag="OHN27_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCQ2287372.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5183 a         5775 c         6063 g         4848 t
ORIGIN      
        1 gccgcgcacc agccacagtg actcgctgcg gctgtaaccg tcctcaccgg gaaacaggtg
       61 ctcaaaatac aggcttctgc cgccgttatc actgagtatc agtgtgttat cgcgcagctg
      121 taagcggata tccgcaggca ttttccagcc ggggccgagg ctccccaccg gcgcgggcgt
      181 ttttgtccgg taactgctgt aggtgcggga gaggatgaac ggcagcgggc cgggcagggc
      241 gatgtcggtt tcaccgggaa ggacctttgc accgagcagg ggattgaccg gatggccgga
      301 cgtcactccg ccggggcaca ccgaacaggc cacgccggtg ggggcaccaa tacgtacccc
      361 ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg tcaccctgac gcgccgccgg
      421 ttttccgctc atcgtaaaat ccttttctat tattttaatg acagtatctt catacgaaaa
      481 agcgaagaaa aacttgtggt cacaatgaat tgaagatttt taggaatata tttagaacgt
      541 tacaaaagag aaggttcgga tagggtattt agagaggaat caggtgtgct agtggccctt
      601 tgttgatcgt tcaccaaagg acccaataat agactcaatc ttccttcggc aagcactgca
      661 aatgcccgtg ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcac
      721 ccatgtcacc tttcaacacg gcgatttcca gacagtcgtc gtggttgatg tgcacatgca
      781 gcgtggcgac ggagaggtcg tggtgatgat gctgggtgga gacaatgcgg ctggctaagt
      841 cgcgtttttc gtgttcatac acatacgaca gcaccgcgaa accttgcgtg ccgtgctgct
      901 gggtggcctc ttgcgccagg gcgctacgca gaatgtcgcg gatagcttcg gaacggttgt
      961 tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca tcaagcgtga
     1021 tggtgactcg ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca cggggaatgc
     1081 gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta atttctctcc
     1141 caccacctgg gtttcgacga tttgtccgtt gtccataacc attacccgct ggcaaaagcg
     1201 ttctaccagg cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact gttgttgtag
     1261 ctttttcagc aggcgaatga cacccgcctg taacacgaga tcaaggttag aaacggcttc
     1321 atccagaatc agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag
     1381 ctggccgccg cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc
     1441 cttcagcatt tcgctggcgc gcgccagttg ttcggatttt ttcagtgata gcaggtggcg
     1501 catcggttca cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc
     1561 ctgaaatacc atctgaatat cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc
     1621 cagcggttcg ccacgccagc taatattccc ctgcgcgggc gattctaaac ccaccagcaa
     1681 ccgcgcgagg gtacttttcc cgcagccgct gcgccccagc agggcgacgg tttcgccgct
     1741 tttcagggtc agggaaacgt tattcagcac cgcctgatgt tgatgttttc cgttaaatcc
     1801 accgtgcgca tagtgatggg aaaggccgga gatgttaagt aaagtcatga tgccagctcc
     1861 ataccgtaga gggcgagatg agcggaaacc aggctgcgcg tcactgtatg tttgggggcg
     1921 ttaaacagcg tttctacatc gccctgttcg acaatcttac cgtcagacat caccgccacg
     1981 tcatccgcca gacgcgccac aacgcccata tcatgggtca ccagcagcat tcccggcgct
     2041 tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg
     2101 tcggtggtcg gttcatcggc gatgataaac ggtgattcac acagcaccgc catcgcaatc
     2161 atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg
     2221 gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc
     2281 cctaacgcca ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgcagtgg attaaaggcg
     2341 ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca aggcgaaacc
     2401 ggtttgccat cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc
     2461 agcgtcgcgg cgcaggttaa tgatttcccg ctgccgctac cgccgactaa cgccagcacg
     2521 cgcccgcgtt gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg
     2581 atattacgta gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc agatgagggt
     2641 ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg ataaacagcg
     2701 ccagccccgg ccagaacatt tgcagcggct gggtccagat atactggcgc gcgtcgttaa
     2761 tcatcacgcc ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg
     2821 cgacgtgcag catcatatgg ccgatatcca gcgttgccag caccagcagc gaagggatca
     2881 ccgcgcctgc cagatgatcg acaaacaccc gcacatggcc cgcaccggaa agccgtgacg
     2941 ccagcacaaa ctcgcgttgg cgtagtgaaa tcaccaggct gcgcaccatg cgtgcatacc
     3001 acgcccagtg cgacagggcg atggcgataa ttacgttggt cagcccggtg ccgagcacgc
     3061 caaccataaa gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca
     3121 tggtggcctg atcaacgcgc ccgccaatca acccggcgct gccgccaata accagcccta
     3181 atgtcagcac cagcagcagg caggccatta ccgaaccgag cgacacgcgg gtcgctgcca
     3241 tcagccgcga gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc tgcgcatccg
     3301 gcgaaagcag gcgcgacggc aaatcaatcg cctgggggtc atacggcagc caccactggc
     3361 tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc
     3421 gggaagagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag
     3481 cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag
     3541 cgtaaagcac tggatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc
     3601 gacgcccggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca gttcgccgat
     3661 atgcatcccc acggcggtga tcatcggcag cgaggcattg cgcaggatgt ggcgacgttc
     3721 ggtctgtttg tcgttcaggc cgcgcagacg cgcccaggtg acgtgacgct gaccggcgac
     3781 gtccagcata ctggcgcgca gtaaacgcgc gttaatcgcc agcgacataa aggcaatgga
     3841 aaccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta gccattgcag
     3901 atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca tcgacacgcc
     3961 aagaaacgcg atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc gcgccgccca
     4021 gataccgagc ggtacggaag tgagcagaat taataccagc gccgcacctg caagttccag
     4081 cgtggcgggc aggaagttca gcatatcgtc cagtaccggg cgttggctgg cgaatgagat
     4141 accaaagtca agatgcagcg ccttccacaa ccaggtgccg tactggacgt acagcggctg
     4201 atccagtccc agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga
     4261 cagacgcaaa taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat
     4321 caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa
     4381 cattaaggtt tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag
     4441 gggatgttac ccagctccgg ttttgatacc accatcattg agatgtaact gataggcaga
     4501 taaaccgcct cgtcatgcag acgggtcaga atgtcgcgat acagcgcctg acgttgcgtt
     4561 tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggttt gtcggctaat
     4621 ccttgctgtg cctggaagtc agcgtgtgac ggtacgcgca ttgaactgag gaaggcgtgt
     4681 ggatcatatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga
     4741 cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc
     4801 atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgaa
     4861 agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc
     4921 caaccggctt tttccagcaa cgctttcgct ttttgcggat cgtactggcg cggtttcagg
     4981 ccgaggttgg cgtagggcac agaaggggca aacagggtgt cggcgacctg ctgggtgcca
     5041 tacaacgcgt tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact
     5101 gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc
     5161 tgtgacagtt gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt
     5221 aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg
     5281 gtagtcgggt ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag
     5341 tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac
     5401 ggtccggtgc caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag
     5461 ggagcgataa aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg
     5521 cttttcaggg taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt
     5581 gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc
     5641 gccgcctcgg catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag
     5701 gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa
     5761 ccgtctgcct gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta
     5821 ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt ggtgatttca
     5881 tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg
     5941 cggagtgtgg agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga
     6001 cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt
     6061 gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaagggag
     6121 taggggtgca gatcgcaagg cttaaatttt caagctgaca atggctgact gacagcgagg
     6181 agtgataggt gctgtctacg ctgacgattt gccaggcgct gccgccacgc tgtttaacga
     6241 tggcttcttt gcgcgtccag atgcgccaga acatttccag ttgctgatca ggatgcacgg
     6301 cgtccatctc agcgtgttcc ccgaggctga atacggcgtt cgccagccag cgccagttgg
     6361 cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcactc aacagcaggg
     6421 cgatatcgtc accgctatgg cttaagttga accatagcgg catttccggc gcaaatgcag
     6481 gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg
     6541 cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg
     6601 gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag aactatccga tacatatcag
     6661 ggccaacgtt taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct catcccggta
     6721 accgactttt cggtctgccc ggccccagta aaatcgccag tttgctaccg cctttggtgg
     6781 tttccatcca gattttacac acgctggtta atggcaccga aagtagcatc cctaccgggc
     6841 cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac atccccaggc
     6901 gatggcccat catccgtgat tctaaaatat tgccgatgac catatggacc actaaaaaca
     6961 atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg
     7021 gtacggcgga aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac gccagtactg
     7081 cccacatcag cgcaaactgc acccccatca gctccagccc cagccagacg atgacacctg
     7141 tccataaact gagtagcgtc ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt
     7201 gtaatcctgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt
     7261 ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg
     7321 ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc
     7381 gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt
     7441 taaaaagttt gcgcgtcaac tccttattaa acttcggcag catagagata aattcgttaa
     7501 acgatgccgc cagtacgccg accagcgcag ttagtgcgat cagcatcacc accaccacaa
     7561 tcgtaatggc aatggggcgt tgtactcccc gacgaataaa ccaggtgacg agcgggttga
     7621 ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa
     7681 tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg cccgttttat
     7741 cgggttgagg ggtttccatt ggggcttcct tgtgactttt tgtattaagt gtagcgggag
     7801 tcacgccagc aatattctga ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg
     7861 aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta
     7921 aacggattgc gcctgaattt gcgcattgtc tctatagtca tgtttaactt cgccagctac
     7981 ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt
     8041 agcgccttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgc
     8101 cctcatgccg gacgttacgc cgattcgctg ggacccaaaa agattgtcgt cttcggttta
     8161 tgcggctgct ttttgagcgg tctggggtat ctgacggcag gattaaccgc cagtctgcct
     8221 gtcatcagcc tgttattact ttgcctgggg cgcgtcatcc ttgggattgg gcaaagtttt
     8281 gccggaacgg gatcgaccct atggggcgtt ggcgtggttg gctcgctgca tatcgggcgg
     8341 gtgatttcgt ggaacggcat tgtcacttac ggggcgatgg cgatgggtgc gccgttaggc
     8401 gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taatcattat gggcgtggcg
     8461 ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaaccg
     8521 ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcgct ggcactggct
     8581 tccgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgc taaaggttgg
     8641 gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta
     8701 ttccctaacg gcattaaccg tatcggtggc ttaaacgtag cgatgatttg ctttagcgtt
     8761 gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaaatcggc
     8821 gtcttactgg cgggggccgg gttttcgctg gtgttcccgg cattgggtgt agtggcggta
     8881 aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt atttatggat
     8941 ttatcgcttg gcgtgactgg accactggct gggctggtga tgagctgggc gggcgtaccg
     9001 gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta
     9061 aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa cttactgaat
     9121 aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat cgtcagcggg
     9181 cagcgtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta
     9241 cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag
     9301 ctcaatggct ttattggtta ccacttgcag ctgcggatcg cggctacgtt gctgatcttc
     9361 cagacgcttc gccagcaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac
     9421 tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt
     9481 accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt
     9541 gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc
     9601 tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag
     9661 attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat
     9721 cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc
     9781 cagtcttttc aacagcatat tgagtaatac gccatacatt ggcaggaaga aaacgatact
     9841 gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa
     9901 ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc
     9961 gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa
    10021 aacgtgcacg tcgaggattt gccccagcgc gtaggccatg aaactggcgg tggcgatacg
    10081 ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccaggaac ccatatagaa
    10141 tagcgacgag atgacgtagg agattaataa cgcagggatc attaccgcga agataatgcg
    10201 tcgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagaa aaataaacgg
    10261 aaagctaaac gcgccccagg tggtatggaa acccaaaatg gagacgggaa gctgaaccag
    10321 atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca acgccttata
    10381 gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg
    10441 gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc
    10501 gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt
    10561 taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt tatgtgatga
    10621 gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac ttggcctgcg
    10681 ctgcccggaa cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc ccggcgaaac
    10741 gttgctgatt atcgccgacg atccggccac tacccgcgat attcctgggt tttgtacctt
    10801 tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt atttgattcg
    10861 taaaggcggt tgatgggggc tgattggctt cgatgccgcc ttttcccctc accctaaccc
    10921 tctccccaga ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct
    10981 cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg tatctgccag
    11041 caccgccagc catagtccgg tcatccctaa aagcgtggtg acgaggaaga tccctttcag
    11101 tcccagcgca atggtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat
    11161 catttgcacc aggccgcgca ggtagttatg ggttaatgct gcatcagcgg tttccagcgc
    11221 cacgtctgtg ccactgccca ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc
    11281 gttaataccg tcaccgacca tcgccagcgg cgcatgttga ttcagctcgg tcaccgcttt
    11341 gactttatct tccggcaaca ggcccgcttt aaactccagc cccagctccc cggcaattgc
    11401 cgccgctgcg cgtggattat cgccggtgag gatcacccct ttgacgccca gcgcgttcag
    11461 ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgtaacgcaa tgacaccaag
    11521 cacgtcatcg ttacgtacta ccagcactac cgtttgcccg gcgctttcca gttcgttaat
    11581 taaaccagca aatgcatcag cgggatgttt cccggcagcg caaatcaata cgcgctcacc
    11641 gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg cggcgggaat
    11701 ggcgagtgca gcaacctgtg cttcgcgcac gatcgcctgc gccagtggat gcgtcgcgcc
    11761 ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg
    11821 aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacctg
    11881 ggtaacacga cctagctgct ccagcgccgc accgccttta attaacgccc cacgacgtgc
    11941 cgctgctgcc agcccggagg taatcgccgc gggcgtggag ataactaacg cacacgggca
    12001 gccaatcagc agcagcgtca gccctttata aatccactct tgccagctgg cggcaaacag
    12061 cagcggcggc accagcgtta ccagcagggc gacggccata attgctggcg tataaatacg
    12121 gctgaaacgg tcgataaacc gctcaatggg ggcgcggcgc tcttcggctt cttcgatcag
    12181 tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag
    12241 acggtctacg ctggtggctc ctgcaggaac tttatcgccc gttgcgcgct ccaccggaat
    12301 ggattcgccg gtcagggcgc tttcatcaaa actggcaaag ggcgagagca gtttaccgtc
    12361 ggcaggcaaa cgcccgcccg ccgcaacttc aatcacatcg ccagggcgca ggctgttaat
    12421 cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat
    12481 taacgcgcta acgccctgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat
    12541 caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca gcgcaccaat
    12601 agcagctacg ctcattaacg tttcaatggc gaagtaactg cctgatttga tcagccgtaa
    12661 tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg ccagttgccc
    12721 gaacggatga ttgaactgct ccagacccca gctgattgcc atcatcacga ttagcgtaat
    12781 cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcacg
    12841 cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacggatgt cattgtcggc
    12901 atcaaccacc agtttttcgg tggcgaacag tacctgcacc tgattcacgc ctgcaagctg
    12961 gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccgc tgactttcca
    13021 gctatagcgg gtgccggaga cgttttcaga gagcgttggc gtgctggaac atgcgccgtc
    13081 gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcgg caaattgagg
    13141 ggctttcttg ccgtgattgt caggagtcga catggcatcc tccggttaag ttttttctca
    13201 ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagcg
    13261 agcggacgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac
    13321 ggaagcgtcg gcgatagtgg ctacccagcc agacaaagtt actgataaac agcaacgaag
    13381 cgccgacaaa cgcagagagc gccggagcgg tcggacggaa gaaccacagt tcacctacca
    13441 gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga tactcttcca
    13501 gccgcgtcca gataatcgcc agtaacagcg cgcccagaac cagcagtacc aacggcagag
    13561 gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga
    13621 ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc
    13681 ccagcagtga ggcgcacagc cccgccagca ccagatagct aatggcgtcg aacatcggcg
    13741 cttgccaggc cagtaacaac aggagaagaa gggttaacgg tttaaacacc cagcgttgcc
    13801 aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa
    13861 acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg
    13921 tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat
    13981 gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct tttctttatt
    14041 gttatcattg gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa
    14101 aaagctgata atgatatggc tccgctccag caaaagctgg tggtggtgag caacaagcgg
    14161 gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt
    14221 atgcgctatg aggcaagctt caaaccgcaa agcggaggaa tggagcaaac gtttcgcctc
    14281 gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga
    14341 acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc
    14401 tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gcaccttttc ggcggtggtc
    14461 atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagctgcat accatgcatc
    14521 agcacggtaa aaatcagcgc cacgttaacg aaaatattca gcgggcgcgg gaagggatgc
    14581 accaggttga ggattaaaaa tccccaaacg cagagcatta acaaacgtcc aatattaatc
    14641 agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct gacccgccac
    14701 tttttcccga tgcaatgacc agtttgccgg aacagtgggc agaccgtttt cgacttcgct
    14761 ttcgacataa atcagggctt cgtcagccag ccagccgtta tcttccagta aatttatcgt
    14821 ctcttctaac aagccacggc ggaacggtgg atcgacaaac actatattat gcggtgtgcc
    14881 tttttgcgcc aggaatgaca tcgcgttgct gttcactacg cgtgcattgc ctgcttttag
    14941 tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa tcaacgtggc
    15001 ctccgcagcg tagcgtgata acgcttccag ccccagcgcg ccgcttccgg cgaagcaatc
    15061 cagacattgg gcgtcaacaa tgaccggagc cagccagtta aataacgttt cgcgtacgcg
    15121 gtcggtggtg gggcgcagac ctgggctatc aggaaccggt agtttacggc ctcgccactg
    15181 cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg
    15241 ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga
    15301 ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac
    15361 agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg
    15421 gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaccgg
    15481 ttgtagaaga aatcgttcag gcgcaagagc ctgcgaaggc ttctgaacaa gccgtcgaag
    15541 agcaaccgca ggcgcatact gaagccgagg cggaaacttt tgctgccgac gttgtggaag
    15601 tcactgaaca ggttgctgaa agtgaaaaag cgcagcctga agcggaagtc gttgcacagc
    15661 cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt
    15721 tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa
    15781 ccgtagagat tgtcgaagcg gcggaagaag aagcggctaa agaagaaatc accgacgaag
    15841 agctggaagc acaggcgctg gctgccgaag cggcagaaga ggcggtgatg gtggttcctc
    15901 cggtagaaga gcagccggtg gaagaaatcg ctcaggagca ggaaaaaccg accaaagaag
    15961 gtttcttcgc gcgcctgaaa cgcagcctgt taaaaaccaa agaaaatctc ggttccggat
    16021 ttatcagcct gttccgcggt aaaaaaatcg acgatgatct gtttgaggag ctggaagaac
    16081 agctgttgat cgccgatgtg ggcgtggaaa ccacacgtaa aattatcacc aatctgacgg
    16141 aaggcgcatc ccgcaagcag cttcgtgatg ccgaggcgct ctatggcctg ctgaaagaag
    16201 agatgggcga gattctggcg aaagtcgatg agccgctgaa tgttgaaggt aaaacgccgt
    16261 tcgtgatcct gatggtgggc gtcaacggtg tgggtaaaac cacgacgatt ggtaagctgg
    16321 cgcgtcagtt tgagcagcag ggtaaatcgg tgatgctggc ggcgggcgat actttccgtg
    16381 cagcagcggt tgaacagctt caggtctggg gtcagcgcaa caatattccg gtgattgccc
    16441 agcataccgg tgcggattcc gcctctgtta tcttcgacgc cattcaggca gctaaagcgc
    16501 gtaatatcga cgtcctgatt gccgataccg ccggacgcct gcagaacaaa tcgcatctga
    16561 tggaagagtt gaagaaaatc gtccgcgtga tgaagaaact cgacgttgaa gcgccgcatg
    16621 aagttatgct gactattgat gccagtaccg ggcagaacgc ggtaagccag gccaaactgt
    16681 tccacgaagc cgttggctta accggcatca cgctaacgaa actggacggc acggcgaaag
    16741 gcggggtaat tttctcggtg gctgaccagt ttggtatccc tatccgctac attggtgtcg
    16801 gcgaacgtat tgaggatttg cgtccgttta aggcggacga ctttatagag gcactttttg
    16861 cccgagagga ttaacaatga ttcgctttga acatgtcagc aaggcttatc tcggtgggag
    16921 acaggcgctg cagggcgtta cgttccatat gcagccgggt gagatggcgt ttctgaccgg
    16981 tcattccggc gcagggaaaa gtaccctcct gaagctgatc tgtgggattg agcggcccag
    17041 cgccgggaaa atctggttta gcggccatga catcacgcgt ctgaaaaacc gtgaagttcc
    17101 gtttctgcgc cgccagattg gcatgatttt ccaggatcac catctactga tggaccgtac
    17161 tgtctacgat aacgtggcga tcccgctgat tatcgctggt gccagcggtg acgatattcg
    17221 tcgccgggtg tcggcggcac tggataaagt cgggctactg gacaaagcga agaacttccc
    17281 tattcagctt tccggcggtg aacaacagcg tgttggcatt gcccgcgcgg tggtgaataa
    17341 gcccgcggta ctgctggcgg acgaaccgac cggtaacctg gacgacgcgt tgtcggaagg
    17401 gattctacgt ctgtttgaag agtttaaccg cgttggggta accgtattga tggcaacgca
    17461 cgacatcaac ctgatttcac ggcgttccta tcgcatgctc accctgagcg atggtcactt
    17521 gcatggaggc gtgggccatg aataagcgcg atgcaatcaa tcatattcgg cagtttggtg
    17581 ggcgtctgga tcgcttccgt aaatcggtcg gcggctcagg cgacggcggt cgtaacgcac
    17641 cgaaacgcgc gaaatcctcg ccaaaaccgg taaatcgcaa aaccaacgtt ttcaacgaac
    17701 aggtgcgcta tgccttccac ggcgcattgc aggatctgaa aagcaaaccg ttcgccacgt
    17761 ttttaacggt gatggttatc gccatctccc tgacgctgcc cagcgtctgt tatatggtgt
    17821 acaaaaacgt taatcaggcg gcgacgcagt attatccgtc accgcaaatc actgtttact
    17881 tgcaaaaaac gctggacgat gacgccgctg cgggcgtggt ggcacagttg caggccgagc
    17941 aaggcgtgga gaaagtgaac tatctttctc gtgaagacgc actgggcgag ttccgtaact
    18001 ggtctggttt tggtggtgcg ctggatatgc tggaagaaaa cccgcttccg gcagtggcgg
    18061 tggtgatccc gaaactcgat tttcagggga cggaatcact caatacgctg cgtgatcgta
    18121 tcacgcagat taacggcatt gacgaagtgc ggatggatga cagctggttt gcccgtctgg
    18181 cggcgttgac cgggctggtc gggcgcgttt cggcgatgat cggcgtgttg atggtggcgg
    18241 cagtgttcct cgtcatcggt aacagtgtac gcctgagtat ctttgctcgt cgtgactcca
    18301 ttaacgtgca gaaactgatt ggtgcaacgg atggtttcat cctgcgcccg ttcctgtacg
    18361 gtggcgcatt gctggggttt tctggcgcat tgttgtcact aattttgtca gaaattctgg
    18421 tactgcgatt gtcatcggcg gttgcggaag tggcacaggt tttcggaacg aagtttgata
    18481 tcaatggctt atcgttcgat gaatgcctgc tattgctgct ggtatgctcg atgattggct
    18541 gggtggcagc gtggcttgcc acggtacaac atttacgcca ctttacgcct gaataataaa
    18601 agcgtgttat actctttccc tgcaatgggt tccgtagcag ggaaagagac cccgttgtct
    18661 cttcccggta tttcatctct atgtcacatt ttgtgcgtaa tttattcaca agcttgcatt
    18721 gaacttgtgg ataaaatcac ggtctgataa aacagtgaat gataacctcg ttgctcttaa
    18781 gctctggcac agttgttgct accactgaag cgccagaaga tatcgattga gaggatttga
    18841 atgactgaca aaatgcaaag tttagcttta gccccagttg gcaacctgga ttcctacatc
    18901 cgggcagcta acgcgtggcc gatgttgtcg gctgacgagg agcgggcgct ggctgaaaag
    18961 ctgcattacc atggcgatct ggaagcagct aaaacgctga tcctgtctca cctgcggttt
    19021 gttgttcata ttgctcgtaa ttatgcgggc tatggcctgc cacaggcgga tttgattcag
    19081 gaaggtaaca tcggcctgat gaaagcagtg cgccgtttta acccggaagt gggtgtgcgc
    19141 ctggtctcct tcgccgttca ctggatcaaa gcagagatcc acgaatacgt cctgcgtaac
    19201 tggcgtattg tcaaagttgc gaccaccaaa gcgcagcgca aactgttctt caacctgcgt
    19261 aaaaccaagc agcgtctggg ctggtttaac caggatgaag tcgaaatggt ggcccgtgaa
    19321 ctgggcgtaa ccagcaaaga cgttcgtgag atggaatcac gtatggcggc acaggacatg
    19381 acctttgacc tgtcttccga cgacgattcc gacagccagc cgatggcacc ggtgctctat
    19441 ctgcaggata aatcatctaa ctttgccgac ggcatcgaag atgataactg ggaagagcag
    19501 gcggcaaacc gtctgaccga cgcgatgcaa ggtctggacg agcgtagcca ggacatcatc
    19561 cgcgcgcgct ggctggacga agacaacaag tccacgttgc aggaactggc tgaccgttac
    19621 ggcgtttccg ctgagcgtgt gcgccagctg gaaaagaacg cgatgaaaaa attgcgtgct
    19681 gctatagaag cgtaatttcc gctattaagc agagaaccct ggatgcgagt ccggggtttt
    19741 tgttttttgg gcctctgtaa taatcaattt cccctccggc aaaacgccaa tccccacgca
    19801 gattgttaat aaactgtcaa aatagctatt ccaatatcat aaaaatcggg tatgttttag
    19861 cagagtatgc tgctaaagca cgggtagtca tgcataaaac gaaataaagt gctgaacaac
    19921 aacatcacaa cacacgtaat aaccagaaga atggggattc tcaggatgaa cataaagggt
    19981 aaagcgttac tggcaggatg tatcgcgctg gcattcagca atatggctct ggcagaagat
    20041 attaaagtcg cggtcgtggg cgcaatgtcc ggtccggtgg cccagtacgg tgaccaggag
    20101 tttaccggcg cagagcaggc ggttgcggat atcaacgcta aaggcggcat taaaggcaac
    20161 aaactgcaaa tcgtaaaata tgacgatgcc tgtgacccga aacaggcggt tgcggtggcg
    20221 aacaaagtcg ttaacgacgg cattaaatac gtgattggtc acctctgttc ttcatcaacg
    20281 cagcctgcgt ctgatatcta cgaagacgaa ggcattttaa tgatcacccc agcggcaacc
    20341 gcgccggagc tgaccgcccg tagctatcag ctgatcctgc gcaccaccgg cctggactcc
    20401 gaccaggggc cgacggcggc gaaatatatt cttgagaaag tgaaaccgca gcgtattgct
    20461 atcgttcacg acaaacagca atacggcgaa ggtctggcgc gagcggtgca ggacggcctg
    20521 aagaaaggca atgctaacgt ggtgttcttt gatggtatca ccgccgggga aaaagatttc
    20581 tcaacgctgg tggcgcgtct gaaaaaagag aatatcgact tcgtttacta cggcggttat
    20641 cacccggaaa tggggcaaat cctgcgtcag gcacgcgcgg cagggctgaa aactcagttt
    20701 atggggccgg aaggtgtggc taacgtttcg ctgtctaaca ttgcgggcga atcagcggaa
    20761 gggctgctgg tgaccaagcc gaagaactac gatcaggttc cggcgaacaa acccattgtt
    20821 gacgcgatca aagcgaaaaa acaggaccca agcggcgcat tcgtctggac cacctacgcc
    20881 gcgctgcaat ctttgcaggc gggcctgaat cagtctgacg atccggctga aatcgccaaa
    20941 tacctgaaag cgaactccgt ggataccgta atgggaccgc tgacgtggga tgagaaaggc
    21001 gatctgaaag gctttgagtt cggcgtattt gactggcacg ccaacggcac ggcgaccgat
    21061 gcgaagtaat cattaatcgg caactttggg ttgccgccaa attgctaata tcgagtacgt
    21121 tgcttcatgc cggatgcggc gtaaacgcct tatccggcct acaagatcca aagaaatcag
    21181 taaattgcaa cacacattgt aggcctgata agcgtagcgc atcaggcaat ttctcgttgg
    21241 tcatcacttt tgaaatcaac ttgacgatta acgcttctcc cagccgccct gttgcgccgt
    21301 aaatcccagt gcctgcataa acgccgtcat cacaccgcga tcttccacgc ctgcatccgc
    21361 catccaccag catgaaacgc caggattgtt acgcaaaacc tcttccagca gatattgccc
    21421 cacaccgcga cgtcgggtga cttcccgcac acgcagggaa tccagtgctc cctcggtgcc
    21481 gcttaaggtt acccgtacgg cagcgagcag gcgctcgtta aaacgcgcgg cgtagatacg
    21541 gtggttatcg tcaacctgta aagaggaagg ggaatactcc ggccagatct tttgcaggtc
    21601 aatccggtcc tggtcgctaa agttttctaa tcgaatgatg gtcagcttca tgggtaaccc
    21661 gtgtaaatca caaaagtgga accagtgtag cgaaataatt taatcggagg ctttctcttt
    21721 tttatttctt ttggcaggtg attaattttt taacagcaat aattacaaag ttaaaacatt
    21781 agagaatgaa aaatacccag cataatcccc tgaatggtag tgaattattc cgccctttgt
    21841 gccgttattt tatgctgaca aaggcactt
//
LOCUS       DAJOSY010000075        21632 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJOSY010000075 DAJOSY010000000
VERSION     DAJOSY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.075"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..316)
                     /locus_tag="OHN27_002975"
     CDS             complement(<1..316)
                     /locus_tag="OHN27_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCQ2287373.1"
                     /translation="MRYNGLNNMFFPLCLINDNHSVTSPSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIASYANNK
                     CDD"
     gene            complement(493..2142)
                     /gene="actP"
                     /locus_tag="OHN27_002976"
     CDS             complement(493..2142)
                     /gene="actP"
                     /locus_tag="OHN27_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCQ2287374.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(2139..2453)
                     /locus_tag="OHN27_002977"
     CDS             complement(2139..2453)
                     /locus_tag="OHN27_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCQ2287375.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2654..4612)
                     /gene="acs"
                     /locus_tag="OHN27_002978"
     CDS             complement(2654..4612)
                     /gene="acs"
                     /locus_tag="OHN27_002978"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCQ2287376.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            5005..6441
                     /gene="nrfA"
                     /locus_tag="OHN27_002979"
     CDS             5005..6441
                     /gene="nrfA"
                     /locus_tag="OHN27_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCQ2287377.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6486..7052
                     /gene="nrfB"
                     /locus_tag="OHN27_002980"
     CDS             6486..7052
                     /gene="nrfB"
                     /locus_tag="OHN27_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCQ2287378.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            7049..7720
                     /gene="nrfC"
                     /locus_tag="OHN27_002981"
     CDS             7049..7720
                     /gene="nrfC"
                     /locus_tag="OHN27_002981"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCQ2287379.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYSVPFKY
                     GEVSQ"
     gene            7717..8673
                     /gene="nrfD"
                     /locus_tag="OHN27_002982"
     CDS             7717..8673
                     /gene="nrfD"
                     /locus_tag="OHN27_002982"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCQ2287380.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8693..10411
                     /locus_tag="OHN27_002983"
     CDS             8693..10411
                     /locus_tag="OHN27_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCQ2287381.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRSEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGVVVSSVSRQEISLN
                     LQPGQPVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGLDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10404..10787
                     /gene="nrfF"
                     /locus_tag="OHN27_002984"
     CDS             10404..10787
                     /gene="nrfF"
                     /locus_tag="OHN27_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCQ2287382.1"
                     /translation="MSKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10784..11380
                     /gene="nrfG"
                     /locus_tag="OHN27_002985"
     CDS             10784..11380
                     /gene="nrfG"
                     /locus_tag="OHN27_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCQ2287383.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     HGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11722..13035
                     /gene="gltP"
                     /locus_tag="OHN27_002986"
     CDS             11722..13035
                     /gene="gltP"
                     /locus_tag="OHN27_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCQ2287384.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(13565..14254)
                     /locus_tag="OHN27_002987"
     CDS             complement(13565..14254)
                     /locus_tag="OHN27_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCQ2287385.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(14348..16495)
                     /gene="fdhF"
                     /locus_tag="OHN27_002988"
     CDS             complement(14348..16495)
                     /gene="fdhF"
                     /locus_tag="OHN27_002988"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(16076..16078),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCQ2287386.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16693..18159)
                     /gene="mdtP"
                     /locus_tag="OHN27_002989"
     CDS             complement(16693..18159)
                     /gene="mdtP"
                     /locus_tag="OHN27_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCQ2287387.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYSMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLATGVAQLYYSMQASYQMLDLLEQTHDVIDYTV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IRPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLHTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAGPVVEKK"
     gene            complement(18156..20207)
                     /gene="mdtO"
                     /locus_tag="OHN27_002990"
     CDS             complement(18156..20207)
                     /gene="mdtO"
                     /locus_tag="OHN27_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCQ2287388.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQNAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHSIAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(20207..21238)
                     /gene="mdtN"
                     /locus_tag="OHN27_002991"
     CDS             complement(20207..21238)
                     /gene="mdtN"
                     /locus_tag="OHN27_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCQ2287389.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(21257..21532)
                     /locus_tag="OHN27_002992"
     CDS             complement(21257..21532)
                     /locus_tag="OHN27_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287390.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4905 a         5673 c         5874 g         5180 t
ORIGIN      
        1 tatcgtcaca tttattattt gcatatgaag ctatatctat aaaatattta tgtattttcc
       61 tgtccgtttc tttcgtgatt tttcctgatg gtaaactatc ataaattctt ttcattttct
      121 gaatcaagcc tatcactttt tcatgatcat ccattttgaa aagagagagg gctttattgt
      181 caattaacgt gtttttatga tgtttgctgt aattatctga ttttgttttc tttgtatgtg
      241 atggacttgt gacagagtgg ttatcgttaa tcaggcaaag agggaaaaac atattattta
      301 aaccattata gcgcataaaa tatccttata attaacaaga aagaaaaggc atattctctg
      361 cattatcatt ttctacgact gtcaaaaatc gctcattttt aataagttta tttgtttaat
      421 attatgggaa aaggtgatgc atgttggaga ggaagagtat tccccggtca ggcgaccggg
      481 gaaggggaga gattaatgcg cgcggccttg ctcaacgcca aagccggtct gggagcggat
      541 aaactgcgcg cggaacagtt cacgctcacg cgcgccttcc gctgagttat cggttgccga
      601 gaagaaccag atgccgagga atgccacggt gatagagaac agcgccgggt attcatacgg
      661 gaagatggct ttttcgtgac caaggatctg tacccaaatc gtcgggccga ggatcatcag
      721 tactactgcg gtaatcagcc ccagccagcc acccatcatc gcgccacgcg tggtcagttt
      781 cgaccagtac atagaaagca gaatgatcgg gaagttacag ctcgccgcga tggcaaacgc
      841 cagccccacc ataaaggcga tgttctgatt ctcaaacagc acgccgagga taatcgcaat
      901 cacgccgagg atcagtacgg tgatttttga tacccgcagc tcttcacgtt cggtcgcgcc
      961 ttttttgaag acgttagcgt acaagtcatg cgaaaccgcg gatgcgcccg ccagcgtcag
     1021 acccgcaacc accgcgagga tagtggcgaa agcaaccgct gaaataaaac cgaggaacag
     1081 gttgccgccc actgcattcg ccaggtgaac ggccgccatg ttgttaccac caatcagatg
     1141 gcccgccgcg tctttatatt ccggattcgc accaaccagc atgatcgcgc cgaagccgat
     1201 aataaaggtc agaatataga agtagcccat aaacccggtg gcgtagaaca cgctcttacg
     1261 tgcttcgcgg gcatcgctga ctgtaaagaa gcgcatcaga atgtgcggca agcccgccgt
     1321 accaaacatc agtcccagac ccagagagag cgcggagatc ggatctttca ccagcccgcc
     1381 cggcttcatg atgtcgacac ctttcgggtg taccgccatc gcttcactga acagattgtt
     1441 gaagctaaag ccgacgtgtt tcatcaccat aaaggccata aagctggcac cgaacagcaa
     1501 cagaacggct ttaataattt gtacccaggt ggtcgccagc atgccgccga acaggacgta
     1561 catcatcatc agcacaccga ccagcaccac cgcaatgtga tagttaaggc caaacagcag
     1621 ctcgatcagt ttacctgcgc ctaccatctg ggcgataagg taaagcgcca ccaccactag
     1681 agaaccacag gccgaaagaa tacgaatcgg cccttgtttc aggcggtaag aggctacatc
     1741 ggcaaaggtg tagcgtccca ggttacgcag acgttcggca atcaggaaca aaatgatcgg
     1801 ccagcccacc aggaagccca gcgagtaaat taagccgtca tagccggagg taaacaccag
     1861 cgcggagatc cccaagaatg aggcggcgga catatagtcc ccggcaatcg ccagcccgtt
     1921 ctggaagcca gtgatattgc cgcctgcggt gtagtagtcg ctacgagaac gtacgcgttt
     1981 tgatgcccag taggtaatgc cgagcgtaaa cacgacgaaa atcaggaaca taataatcgc
     2041 ctgccagttc gttggctggc gctctacggc cccgctaata gcatccgcgg cgttagctgc
     2101 gaaagggagt gtggcggcaa gcgccgtcag aactcttttc atgatgcttg tacctcatgc
     2161 aggacttcat tattaagacg gtcgaattcg ccgttcgccc gccagatgta gataccggtg
     2221 agaacaaagg agatcacaat cactccaaca ccaattggaa taccgcgtgt gacgctggtg
     2281 ttcggattca gcggcgtgcc cagccagccg ggcgcgaagg cgatcagtaa aataaagccg
     2341 atataaactg ccagcataat aatcgacagg atggtggcaa accgttgccg tttttcgact
     2401 aactccctga aatgcgcatt gtcttctatc cgctgataaa tagtgccatt catcacagat
     2461 tctccagagg taatgtaggg attgttttaa ttcccgctcc cttatgggag aaggttaacg
     2521 ctcgggtaac ccttgccgaa tgtaggccgg ataaggcgtt atacgccgca tccggcaatc
     2581 aatgcctgat gcgacgctgt cgcgtcttat caggcctaca aaccgttacc gactcgcatc
     2641 cggcaattgt gggttacgat ggcatcgcga tagcctgctt ctcttcaagc agcttctcga
     2701 ctacgccagg atcggcaagc gtcgaggtat cgcccaggtt gctggtatcg cccgccgcaa
     2761 ttttgcgcag aatacggcgc ataattttgc cggagcgggt tttaggcagg gagtcggtcc
     2821 agtgcagcac gtctggcgtc gccagcgggc caatctcttt acgcacccag ttgcggactt
     2881 ctgcgtacag ttctggtgac ggttcctccc cgtgattaag cgtgacgtag gcgtagatcg
     2941 cctgaccttt aatattgtgc ggaataccta ctacggcggc ttcggcaatc ttcggatgcg
     3001 ccaccagcgc cgactcaatc tccgccgttc ccagacggtg accggagacg ttcagcacat
     3061 cgtccacacg cccggttatc cagtaatagc catcttcatc gcgacgcgcg ccgtcgccgc
     3121 tgaaatacat atttttgaag gtggagaagt aggtctgttc aaaacgttcg tgatcgccaa
     3181 acagcgtacg cgcctgaccc ggccaggagt cggtgatcac caggctgcct tcggtagcgc
     3241 cttccagcgg gttaccttcg ttatcgacca gcgccggttg cacgccgaag aacggacgtg
     3301 ttgccgaacc ggctttcagc tcggtagcgc caggcagcgg cgtgatcatg aaaccgccag
     3361 tttcggtctg ccaccaggta tcgaccaccg gacatttctc gttgccgatt tttttccagt
     3421 accactccca cgcttccggg ttaattggct cgcccacgga accgagaatg cgcagcgacg
     3481 aacggtcggt accctcgatc gctttatcgc cttccgccat cagcgcgcgg atcgccgtgg
     3541 gtgcggtata gagaatattg acctgatgct tgtccaccac ctgcgccata cgggcaggcg
     3601 tcggccagtt cggtacgcct tcaaacatca gcgtggtcgc accgcaggcc agcgggccgt
     3661 acagcaagta actgtgtccg gtcacccagc ccacatcggc ggtgcaccag tagatatcac
     3721 ccggatgata atcaaagaca tatttaaagg tcagcgccgc gtacaccaga taaccgccgg
     3781 tagtatgcag cacacctttt ggcttaccgg tagaaccgga ggtgtagaga ataaacagcg
     3841 gatcttcggc gttcatctct tccgcctggt gctgatcgct cgcttgctca accaggtcgt
     3901 gccaccacag gtcgcgccct tcctgccagt caattttccc gccagtacgc ttcagtacca
     3961 ccacatgctc tacgctggtg acgttcgggt ttttcagcgc gtcatcaacg tttttcttca
     4021 gcggaatact gcgcccggca cgcacacctt cgtcggaagt gatcaccagt cgtgagttgg
     4081 aatcaataat gcgcccggca acggcttccg gcgagaagcc gccgaaaatc accgaatgca
     4141 ccgcgccaat gcgggcgcag gccagcatcg caaccgcggc ttccggcacc atcggcatat
     4201 aaatcgccac cacatcacct tttttaatgc ccagctcgag cagggtattg gcgaagcggc
     4261 agacgtcgcg gtgcagctct ttatagctga tatgtttgct ctggctggcg tcgtcgcctt
     4321 cccagatgat ggcggtacga tcgccgtttt cttgcagatg gcggtcaagg cagtttgccg
     4381 ccagattcag cgtgccgtcc tcgtaccatt taatggacac attaccgggg gcaaaggagg
     4441 tgtttttcac cttctggtaa ggtttgatcc agtcaagaat ttttccctgt tcgccccaga
     4501 aggtatcagg tacgttaata gattgttgat acatcgcctc gtactgctga gggtttatca
     4561 ggcaacggtc tgcgatgttg gcaggaatgg tgtgtttgtg aatttggctc atgcttttgt
     4621 tctccttgta ggatgttaat aatatgtggc ataagcgtta aatgtagggg tattggcagt
     4681 tttgtttagt atttgggcga cagatcacgc aaaagtagaa ttgtgcaaat aaacggcagg
     4741 gtaatttttg aaggtcagca acaaaagttg attaattctt tgaggaacat gcagttatgc
     4801 atgctgttga aaaagaggaa gatactgact aactctaaag tggtatttta catgcactta
     4861 caattgatta aagacaacat tcacagtgtg gttatttgtt acacataggg gcgagcaatg
     4921 tcatgacagt gtaggtgcgg ttactgtcgt gaaaaacaat aaaaaccgcc attgcaacaa
     4981 tggcgcaatt cggatgaagc ccctatgaca aggataaaaa taaacgcacg ccgtatcttc
     5041 agcttattga ttcctttttt ctttttcact tctgttcacg ctgaacaaac ggctgctccc
     5101 gcaaaacctg taactgtgga agcgaagaat gaaacctttg ctccgcagca tcccgatcaa
     5161 tatctctcct ggaaagccac ctcggaacag tcagagcgtg ttgacgccct ggcggaagat
     5221 ccacggctgg tgatcctgtg ggcggggtat cccttctcgc gcgattacaa caagccgcgt
     5281 ggacatgctt ttgctgtgac cgatgtgcgt gaaaccctgc gtaccggtgc gccgaaaaac
     5341 gctgaagatg gtccgctacc gatggcatgc tggagttgta aaagcccgga tgtggcgcgt
     5401 ctgatccaga aagacggcga agatggctac ttccacggta aatgggcgcg cggcggtccg
     5461 gaaatcgtca acaacttagg ttgtgccgat tgccataaca ccgcctctcc agagttcgcc
     5521 aaaggcaaac cggagttaac cctttcccgt ccgtatgcgg ctcgcgcgat ggaagccatt
     5581 ggtaaacctt ttgagaaagc cggacgtttc gaccagcaat cgatggtttg cggtcagtgc
     5641 catgtggagt attacttcga cggcaaaaac aaagcggtta aattcccgtg ggatgacggc
     5701 atgaaagtcg aaaatatgga gcagtattac gacaaaattg ccttctctga ctggactaac
     5761 tccctgtcga aaacgccaat gctgaaagcg cagcacccgg aatatgaaac ctggacagcg
     5821 ggcattcacg gtaaaaacaa cgtgacctgt atcgactgcc atatgccaaa agtgcagaac
     5881 gccgaaggca aactctacac cgaccataaa attggtaatc cgtttgataa cttcgcccag
     5941 acttgtgcga actgccatac ccaggacaaa gctgccttgc aaaaagtggt cgcggaacgt
     6001 aagcagtcga ttaacgacct gaaaatcaag gttgaagatc aactggttca cgctcacttc
     6061 gaagcgaaag cagcgctgga tgcaggcgcg acggaagctg aaatgaagcc aattcaggac
     6121 gatatccgtc atgcccagtg gcgctgggat ctggcgatcg cttcccacgg cattcatatg
     6181 cacgcaccgg aagaaggttt acggatgctc ggtacggcga tggataaagc ggcggatgca
     6241 cgcaccaaac tggcgcgcct gctggcgacc aaaggcatca cccatgaaat ccagatcccg
     6301 gatatctcaa ccaaagagaa agcccagcag gccattggcc tgaacatgga acaaatcaag
     6361 gccgagaagc aggacttcat caaaacggtg atcccgcagt gggaagaaca ggcacgtaaa
     6421 aacggtctgt taagccaata accccgttcc gcctcgcaag gggcggaaaa cacaatggag
     6481 tgaatatgag cgtattacgt tcgttattaa ctgccggggt gctggcgtcg ggcctgttgt
     6541 ggagcctgaa cggaattacc gctacccctg cggcgcaggc atctgatgat cgctacgaag
     6601 ttacccagca gcgtaacccg gatgccgcct gtctggactg tcataaacca gataccgaag
     6661 gtatgcatgg aaaacatgct tccgttatca acccgaataa caaactgccg gtcacctgca
     6721 ccaactgtca cggccagcca tcaccgcaac accgcgaagg ggtgaaagat gtgatgcgct
     6781 ttaacgagcc gatgtacaag gttggagagc agaacagcgt ctgtatgtcc tgtcatctgc
     6841 cggaacagtt gcaaaaagcg ttctggccgc acgatgtcca cgtaaccaaa gtggcgtgcg
     6901 ccagctgcca ttccctgcat ccacagcaag atacgatgca gacgttaagt gacaaaggac
     6961 ggattaagat ttgcgtcgat tgccacagcg atcagcgcac caatccgaac tttaacccgg
     7021 cgtctgttcc gttgcttaag gagcagccat gacctggtct cgtcgccagt ttctcaccgg
     7081 cgtcggcgtg ctggcagccg tcagcggcac cgcagggcgc gtggtggcga aaacgttgaa
     7141 tatcaatggg gtgcgttacg gcatggtgca tgacgagtcg ttatgcatcg gctgtaccgc
     7201 ctgtatggat gcttgtcggg aagtgaacaa ggtgccggaa ggcgtctcgc gcctgacgat
     7261 tattcgtagc gagccgcagg gcgaatttcc tgatgtgaaa tatcgcttct tccgtaagtc
     7321 ttgccagcac tgcgatcatg cgccgtgcgt tgacgtctgc ccgaccggtg cgtcttttcg
     7381 cgatgctgcc agtgggattg tcgatgttaa tccggatctc tgcgtcggtt gtcagtactg
     7441 catcgccgcc tgtccgtacc gcgtgcgctt tatccatccg gtcacgaaga cggcggacaa
     7501 atgcgatttc tgccgtaaga ccaatttgca ggccggtaag ttgcccgcgt gcgttgaagc
     7561 ttgcccgacc aaggcgctga cgtttggcaa tctggacgat cccaacagtg agatttcgca
     7621 actgctgcgc cagaagccca cttaccgcta caagctggcg ctgggaacca aaccgaagct
     7681 gtacagcgta ccgtttaaat acggggaggt gagccaatga cgcagacttc cgcatttcat
     7741 tttgaatcgc tggtgtggga ctggccgatt gccatctacc tgtttttgat tggtatttct
     7801 gctggtctgg tgacgctggc cgtgctgtta cgtcgcttct acccgcaggc gggcggtgca
     7861 gacagtacgt tgctgcgcac cacgctgatt gtcgggccgg gcgcggtgat cctcggtctg
     7921 ttgatcctcg tcttccacct gacaagaccg tggaccttct ggaagctgat gttccactac
     7981 agttttacct cggtgatgtc gatgggggtg atgctgtttc agctctacat ggtggtgctg
     8041 gtgctgtggc tggcgaaaat ctttgaacat gatttgcttg ccctgcaaca acgctggttg
     8101 ccgaagctgg ggatcgtgca aaaggttctg agcctgctga cgcccgttca tcgcggactg
     8161 gaaacattga tgctggtgct ggcggtgttg ttgggggctt ataccggctt tctgctgtcg
     8221 gcgctgaaat cgtatccgtt cctcaataac ccgatcctgc cggtgctgtt cctcttctcc
     8281 ggcatctcgt ccggtgcggc ggtggcgctg atcgccatgg cgatacgcca acgcagtaac
     8341 ccgcattcca cggaagcgca gtttgtacac cgtatggaaa tccctgtggt atggggtgaa
     8401 atcttcctgc tggtggcgtt ttttgtcggt ctggcgctgg gcgatgacgg taaagttcgt
     8461 gcgctggtgg cggcattagg tggcggtttc tggacgtggt ggttctggct tggtgtcgcc
     8521 gggctggggc tgattgtgcc aatgttgctc aaaccgtggg tcaatcgcag ttccggcatt
     8581 cctgccgtgc tggcggcgtg tggggccagt ctggtcggcg tgttgatgct gcgctttttc
     8641 attctctacg ccggacagtt gacggtggcg taagccagaa aagaggtggt ttctggacgt
     8701 attccttcct gaagtcggtt ttctggcgtt gttgttaagt ctcggggtca acgtgttgac
     8761 cccgttgacg gcctttgcgg gagtgcggtt gcgctggcct gccatgatgc gactcacttg
     8821 catcggcatt ctggcgcagt tcgcgctcct gctgctcgcc tttggcgtac tgacgtattg
     8881 ttttctcatc agcgatttct cggtcattta tgtcgcgcaa catagctaca gcctgctgtc
     8941 gtgggaactc aagctggcag cggtgtgggg cggccatgaa ggttcgctgc tgctttgggt
     9001 gctgctgctt tccgcctgga gcgcgctgtt tgcctggcat tatcggcagc aaaccgatcc
     9061 gctatttccg ctgacgctag ccgttttatc tctcatgctc gccgcactgc tactgtttgt
     9121 ggtgctgtgg tccgatccct tcgtgcggat atttccacca gcaatcgaag gccgcgatct
     9181 caatccgatg ctgcaacatc ccggtcttat ctttcatcca ccgctgcttt atctcgggta
     9241 tggcggtttg atggtggcgg cgagcgtggc gctggcgagt ttactgcgca gcgagtttga
     9301 tggtgcctgc gcccgaattt gctggcgctg ggcactacct ggctggagcg cattaacggc
     9361 ggggatcatt ctcggttcct ggtgggccta ttgcgaactg ggctggggcg gctggtggtt
     9421 ctgggatccg gtggaaaacg cctctttatt accctggctt tctgccactg cgctgctgca
     9481 cagtttatct ctgacacgtc agcgggggat tttccgccac tggtcgctgc tgctggcgat
     9541 agttactctg atgctgtcgc tgctgggcac cttaattgtc cgttctggca ttctggtttc
     9601 ggttcatgcg ttcgcgctgg ataacgtccg cgccgtgccg ttgttcagcc tgtttgcact
     9661 gattagcctt gcgtctctgg ctctgtatgg ctggcgagcg cgggacggtg gcccggcggt
     9721 gcgtttttcg gggttatcgc gggaaatgtt aatcctcgct acgctgttgc tgttttgcgc
     9781 agtgctactg atcgtgctgg tgggaacgct ttatccgatg atttacggcc tgctgggctg
     9841 gggacgcctc tccgttggcg caccttattt taaccgcgcg acgttaccgt ttggtctgtt
     9901 gatgctggtg gtgattgtgc tggcgacgtt tgtctctggc aaacgcgcgc agcttccggc
     9961 gctggtagct catgcgggcg tgctgttatt tgccgctggg gtcgtggttt ccagcgtcag
    10021 ccgtcaggaa atcagcctga atttacagcc gggtcagccg gtgacgctgg caggatacac
    10081 cttccgtttt gagcgcctcg atctgcaagc caaaggcaat tacaccagcg aaaaagcgat
    10141 agtggcgctg tttgaccatc agcaacgcat tggtgaatta acgccggagc ggcgttttta
    10201 cgaagctcgt cgtcagcaaa tgatggaacc gtcaattcgc tggaacggca tccatgactg
    10261 gtatgcggtc atgggggaga aaactgggct ggatcgttac gcttttcgtt tgtatgtaca
    10321 aagcggtgtg cgctggatct gggggggagg attgttgatg attgcaggcg cattattaag
    10381 cggatggcgg gggaggaagc gcgatgagta aaggacttct cacgctactg ctgttattta
    10441 cctgttttgc gcgcgctcag gttgtagaca cctggcaatt cgctaatccg caacaacagg
    10501 aacaggcgtt aaatattgcc agccagttac gttgtccgca gtgccagaat caaaacttac
    10561 tggaatccaa tgcgccggtg gcagtcagta tgcgccatca ggtttacagc atggtggcgg
    10621 aggggaagaa cgaagtcgaa atcattggct ggatgaccga acgctacgga gattttgttc
    10681 gctataaccc accgttaacg ggtcagacgc tggtgttatg ggcgctgcca gtggtgttgt
    10741 tactactgat ggcactgatc ctctggcgag tgagggcgaa gcgatgaaac agcccaaaat
    10801 accggtgaaa atgctgacaa cactcacaat attgatggta tttctctgtg tcggcagtta
    10861 tctgttaagc ccaaaatggc aggcggtacg tgcggagtat cagcgtcagc gcgatccgct
    10921 acatcagttt gccagccagc aaaccccgga agcgcagctt caggcattac aggataaaat
    10981 ccgcgctaat ccacaaaaca gcgaacagtg ggcgttactg ggcgagtact atctgtggca
    11041 aaacgattac agcaattcgc tgctggcgta tcgtcaggcg ttgcaactac atggtgagaa
    11101 cgctgaactg tatgcggcgc tggcgacggt gctttattac caggccagcc agcatatgac
    11161 cgcccagact cgcgcaatga tcgacaaagc cctcgcgctg gactctaacg aaatcaccgc
    11221 tctgatgctg ctggcttccg atgcgtttat gcaggcgaat tacgcgcaag ccatcgaact
    11281 atggcaaaaa gtgatggatc tcaactcacc ccggatcaac cggacacagc tggtcgagtc
    11341 gattaatatg gcgaaactgt tgcagcggag atcggattaa tcatctggtg atgaagatta
    11401 tgtcattgag ctgcataaaa aataatcgaa tgaacatatg ccaaaaataa tcactaatca
    11461 gtattattgc agattaaaca aataaaaatc tttccataac aaatggttat tcattaatcc
    11521 tgccataaac tcgcgttatt atgcattaat gcagcgaaaa gctctgttgt taaagggttg
    11581 cgcaacatac cgcgcaaatg atactgatca taagcgttaa aaaaatctac aaaccaacgc
    11641 aacacaattc atgccctggc agtatgtcac gttctcgcgt ttctgaacgg ggaacggcgc
    11701 tccattgagg aagttattct gatgaaaagt ataaaattca gcctggcctg gcagattctg
    11761 tttgctatgg tgctgggcat tctcctggga agctacctgc actaccatag cgacagccgc
    11821 gactggctgg tcgtcaattt gctctctccg gcgggtgata tcttcatcca tctgatcaaa
    11881 atgattgttg tgccgattgt gatctccacg ctggtggtgg gtatcgcggg tgttggtgat
    11941 gccaaacagc tcgggcgtat tggcgcgaaa accattattt acttcgaggt gatcaccacc
    12001 gtcgccatca ttttggggat cactctggcg aacgtcttcc agcccggtgc cggggtggat
    12061 atgtcgcagc tggcgaccgt cgatatctcg aaatatcaga gcactacgga agcggtacaa
    12121 agcagttccc acggcattat gggcacgatt ttgtcgctgg tgccgacgaa cattgtggcg
    12181 tcgatggcga aaggcgaaat gctgccgatc atcttcttct cggtgctgtt tggtctgggg
    12241 ctttcctccc tgcccgcgac gcatcgtgaa ccgctggtga ccgtgttccg ctccatctct
    12301 gaaaccatgt ttaaagtgac tcacatggtg atgcgttatg caccggtggg tgtgtttgcg
    12361 ctgattgcgg tgacggtggc taactttggt ttctcttctc tgtggccact ggcgaaactg
    12421 gtgctgctgg tgcatttcgc cattctgttc ttcgcgctgg tagtgctggg aattgtggcg
    12481 cgcctgtgcg ggttaagcgt ctggatcctg attcgtattc tgaaagatga gctgattctg
    12541 gcgtactcca ctgccagctc tgaaagcgta ctgccgcgaa ttattgagaa gatggaagcc
    12601 tacggagcac cggcgtcgat caccagcttc gtggtgccga ccggttactc ttttaacctt
    12661 gatggttcga cgctgtatca aagtattgcc gctattttca tcgctcagct gtatggcatt
    12721 gacctgtcca tctggcagga aatcattctg gtgctgacgc tgatggtgac ctcgaaaggg
    12781 attgctggcg tgccgggagt gtcgtttgtg gtgttgctgg caacgctggg tagcgtaggt
    12841 attccgctgg aaggtctggc gtttattgcc ggtgttgacc gtatcctcga catggcgcgt
    12901 actgcgctga acgttgtggg taatgcgctg gcggtgctgg tgattgccaa gtgggaacac
    12961 aaatttgacc gcaaaaaggc gctggcttat gagcgtgaag tgctgggtaa atttgataaa
    13021 actgcggatc aataattgaa gattgccggg gatatccacc cggcaatgtg tggatgcctg
    13081 atgcgacgct ggtgcgtctt atcaggccta cgccagacag cgcaatagcc tgatttagcg
    13141 tgattttgta ggtcggataa ggcgtttatg ccgcatccga catcaacgcc tgatgcgacg
    13201 cttaacgcgt cttatcaggc ctacgccaga cagcgcaata gcctgattta gcgtgatttt
    13261 gtaggtcgga taaggcgttt acgccgcatc cgacatcaac gcctgatgcg acgcttgccg
    13321 cgtcttatca ggcctacgcc agacagcgca atagcctgat ttagcgtgat tttgtaggtc
    13381 ggataaggcg tttatgccgc atccgacatc aacgcctgat gcgacgctta acgcgtctta
    13441 tcaggcctac gccagacagc gcaatagcct gatttagcgt gattttgtag gtcggataag
    13501 gcgtttacgc cgcatccgac atcaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
    13561 tatcttaacc gttggttaat ttttcaaact cttcacaccc ggtatcaaac ccttccatac
    13621 agctcaggtt caaccagtgc agcgcctttt gcttgttctt ctcgataaaa ccttcttcac
    13681 cgtttaagaa catcattccc gcccagtact cggaataacc ggttcgggaa attgcagagc
    13741 tgcgtttgaa ataccaggtt gccttgtcat cgtcggcctt aatgcccacg ccgttggcgt
    13801 aaatcaaacc aagcagcatt tgggcatcga cggcagagtc gttctccaga tcttccgagg
    13861 cgttttctaa cagcgaaatt gcctttggat aatccggttt acccgcttga gtatttacca
    13921 gaatatgcgc cagcgttact tcaccttctt tactgcccgc ttgcgccgct ttctctgcaa
    13981 gaacttttgc ttgtggatag tccagactga ccggattggt gattttgatc tgcgccagca
    14041 gcgcgcaggc atcagcatca ccgctgtcgg cggctttctg tgcccaatat tcggctttgc
    14101 tcaaatcgcc ggagctaaac cagctgtcgg caagaaaata ttgtgcgcgt cggtccccgg
    14161 cctctgctgc ctttaaatac tggctgccag gctcggagtc gttggcgtgg gcaaagaatg
    14221 tcaaaaacaa catcaatgcg ataatttttt tcattttgat aatacgattg ggtagggagt
    14281 aaccagtata aagagttcga atggataaaa aaacagcctc cgaaaggagg ctgtagaaag
    14341 gacggtatta cgccagtgcc gcttcgcgca ggcgagtttt caacttgttg tactcgtcaa
    14401 tcacgtactg ctcggcggcg cgctgatcgg cgatcggctc gacgcgcacg gcgcagtatt
    14461 tgtattccgg cgttttcgta atcgggctta agttttcggt aaccagctcg ttacaggcac
    14521 caatccacca ctggtaggtc atgtaaatcg cccctttgtt cggacgatcg ctgacctgcg
    14581 cacgggtgat aattttgcct ttacgcgagt gcacccaaac caatgcctca tcttcaatac
    14641 ccagacgttt ggcgtcttcg gtattgattt gtgcgtagcc aggttcatca gccagcgccg
    14701 ccagtgccgc acagttaccg gtcatcgaac ggcaagagta gtgaccaact tcacgcaccg
    14761 ttgacagtac catcgggtac tcgtcggtga gtttgtcgat tggcgctacc cagtcgcagg
    14821 tgaagaactg cgccagaccg ttcggggtat caaacttctc tttaaacaga taagaagtcc
    14881 cctgatcggc atctgaagta tcgcggcaag gccactgaat gaagcccagt tcgcccattt
    14941 tctcgtaagt cgcaccgtag aaatccgggc acagatgacg caactcatcc cagatctcct
    15001 gggtgttgtt gtagtgcatc ggataaccca tacgggtggc gatttcactg atgatttgcc
    15061 agtccgtttt cagatcccat ttcggttcaa ccgccttgaa gaaacgctgg aagccacggt
    15121 cagccgcagt aaacacgcct tcatgctcgc cccacgacgt tgacggtaaa ataacatccg
    15181 ccgccgacgc ggttttggtc ataaagatgt cctgaacgat aaccagttcc agatcttcaa
    15241 aggctttacg tactgccgac agctccgcgt cagtttgcag cggatcttcg cccataatgt
    15301 acgcggcacg cacttcgcca tgcgctgcgc ggtgcggtag ctcgctgatg cgatagccgg
    15361 tatgcgctgg caggctttcc acgccccagg ctttggcgaa tttctcgcgg ttagccggat
    15421 ctttcacgta ctggtatccc ggatacgtat ccggcagcgc gcccatatcg caggcaccct
    15481 gtacgttgtt ctgaccacga accgggttaa cacccgcatg tggcttaccg aggttaccgg
    15541 tcagcatcgc gaggctggtc agagaacgca cggtttccac gccctggtag aactgggtta
    15601 cacccatgcc ccacaggatg gcggcgcttt tcgcctgggc atacatccgt gccgcctgac
    15661 gaatctcact ggcgctgacg ccggtgatat cttcaaccga ctccggcgtg tagccttcaa
    15721 cgattttacg atactcttca aagccttctg tacgggaagc gacgaatgct ttgtcgtaca
    15781 aattttcttc aataatgaca tggcccatcg cattcaacag cgcgatgttc gagccgtttt
    15841 tcagtgcaat gtgcatgtca gcaatgcgcg cggtttcaat tttgcgcgga tcgcagacga
    15901 taattttcgc cccgttacgt ttagcgttaa ttacgtgatt cgctacgatt gggtgggaat
    15961 ccgccgggtt gtacccgaaa acgaacacta aatcggtatt atcaatttcg ttaatagcat
    16021 tgctcattgc gccattaccg accgattggt gcagacctgc aaccgatggg ccgtgtcaga
    16081 cacgagcgca gcagtcaacg ttattggtac caataacggc gcgcgcaaat ttttgcatta
    16141 catagttggt ttcgttaccc gtaccacgcg aggagccggt cgtctggatg gcatccggac
    16201 cgtacttctc tttgatggcg ctcaggcgct cggcaacgta attcagtgcc tcatcccagg
    16261 aaacaggttc gagtttgccg ccacgctggc gacggatcat gggggttttc aggcgcgggg
    16321 tcaggatctg ggtatcgtta atgaagtccc agccataata acccttcaga cacagggtac
    16381 cctggttggt tttcccctgc gctgcctccg cccggacgat tttgccgtta tcgacgacca
    16441 ggttgatttt gcaacctgat gcgcaatagg ggcaaaccgt gacgactttt ttcatcggtc
    16501 tcgctccagt taatcaaatc acgcatacgc gctctcgact acagtatgca tcttttatgc
    16561 cacattttat gtggggtcat tccctgatat tacgggcact atttattcaa aactctgacg
    16621 aaaaacaggc tgtcgtcagt tttgacgtga cgaaacgaaa taccgcgtga cagccatcac
    16681 gcggcagaca ttttattttt tctcgacgac gggacctgcc tgatacccgc cgcccagcga
    16741 tttcatcaac tgaatgcttt ggatcacccg gcggctgtcc agcatcagta atgacatctc
    16801 ttcggcaagc actggcaacc gggcttcggt ggcctgtaag cggctggtta agccgcgctg
    16861 ataggcggcc tcggcagcgc gctgggtaaa gcgcgtggct tccacgcgtt cagcctgcat
    16921 ttctcgctcg tcgttgagcg tttgcagacg cgtgccgttg acggcaacgt cacgcaccgc
    16981 gttcagtact gactggttgt aacgttcaat catcatgttg ctggcggcgc gcgtgccttc
    17041 gagattggca ttcaaccgtc caccgtcaaa cagcggcaat ttcagacccg ggatgaagtt
    17101 gaactggcga ctggtttttt tgaataaggt atgcagatgg atggagtcca gaccgaaaaa
    17161 cgctttgata tcaaagctcg gatagaacaa cgcccgcgcg gaatccacct gatctaatga
    17221 cgcctgaaca taccagcgca tggcttgcag atccgggcgt ctggcgagca actcataaga
    17281 gagtgtcgcc ggaatgccgg tctggacttg cggtaatgcc accggtctga tctccggcat
    17341 atcgctggct cccgcgccaa tcaatgcacg cagagattct cgcgtttcgg tgatttgccc
    17401 tttgacggca acaatttgtt tatcgaccgc cagaatctgt gcccgcgcgc cgtggaaagg
    17461 cacttgcgct tccagaccgt gcgccacttt actctggtgc gctttcaccg tgtaatcaat
    17521 cacatcgtga gtttgttcta acagatcgag catctgatag ctggcctgca tactgtaata
    17581 aagctgcgct acgcccgtgg ccagcgatag ctctactgct gcggtttctg ccagcgcggc
    17641 attatgcgcg ccaatggcgg cggcaaccgc tgagcgatgc acaccccaca aatcaagatc
    17701 cagtccggca aacaaaccta ctgtggcttc cgtatagtac ggcccgtcca tacccagtgc
    17761 tggcgcatcc attgaataag ggcttaaaaa gccgttcgcc gagacacgtt ggcggttgag
    17821 catccctaac gctgcgacct gtaattgtga accggcatct aacaaatcgg cctgggactg
    17881 cgctttttct tcccgcagtt tcgcttcggc gagggtgtgt gaaccactta gcgtccgttg
    17941 gatcagcgca tccagctgcg ggtcattgag ttgtttccac cactgcgcct gcggccagcc
    18001 ggagctggca agatgaatat cgtcggccag tttgatttgt tccggtttga gctgttgatg
    18061 aggtgcagaa tccttacgta ccagggcaca gccggaaatc agcgtcgtgc tgccgagaat
    18121 gctgcacaac agcagacgtg aaagttgacg attgatcatt gcgtggctcc ttgtgcctgt
    18181 tccgtggcgg gcgttaatgc cggggctgtc cagtctggca agcgggcgaa aagctggcag
    18241 acgtgttgct cctgttttaa taaggtgggc aggatggcct gagaggcggg tgtctcttca
    18301 agcgttattt gaggagaacg gctgagtgcg gttgccagac cggcagcgta tttctccagg
    18361 gcgtcggcaa actgacctgc tctgtccggc tgtagtgcgt tatccagcgc ctgcgccgag
    18421 ttccaggtgg catcccaggc gtgaagaaga tcgcggccct gacgaataac cgtttgcgaa
    18481 cgttcaatca gtaatgcgcg ttcttcgctg tccagttgac gctccagcgc cacgcgttgg
    18541 cacatctctt cacaggcatt aaacgccgca tgcagaccta tacgaatttg cagataagtg
    18601 cgcagagccg tgacttcctg ctggcgtggg atccgcatta ctttacttaa catacccagc
    18661 gcgccagcca gtttttgcgg cagcgtgcgc gcttcactct caggccagac aaaggtgtaa
    18721 atcaccgcgg acaccacggt accaatgatg atacccaggg cgcgatcgcg aatttccacc
    18781 aggtcgtaca ccgggccaaa aacgttttcg agcgtggcga gcgcgaaggt gaccaccatc
    18841 tgtgtgccga tataagaaga acgttcagag ctggtggcaa tccatgcgcc caacaggaaa
    18901 ataggtgcca gcacaaacag caattcgaca atattgtcca gccagggcat gaccagtagc
    18961 gtgaataaca gcgccagaat cgcgccgcaa aaagccccgc caaaacgcag caccatcttc
    19021 tggtacgacg aaccgacatt cgggttagcg acgatcacgc aggtcagcat acaggtgtga
    19081 atgccttccc agtcgacgcc gctgtagaag gtgtaacaga tcaaacaggc gagcagcgtt
    19141 tttaccgcgt agcgcatata gtctggattg gtaaaagcat cggcggccat tgatggtggt
    19201 ttggcggcgg gcgttggcgg cgtattcggg tccatctgac ccagttgtaa caacgtctgg
    19261 cagatattct ccaggttaca ttcccgtgcc gccatcgctt cactttcact gatccgccag
    19321 tcgctttgcc agcactgacc ttcggcaatg gaatgctgca gcttgttgat ttctgaggct
    19381 aatttttgtc ggaattcaat aattgcctga gaatcagcaa aagaggtggg atcgtagcga
    19441 ttcagcgtcg agtaaatgta ggttaccgtt gccacgcagc tttgccacca tgcgttttga
    19501 gttcgccagt tggcatcgtc cgcgaggcaa aagacattga gtttttgtag cgccagcgcc
    19561 tctctttcaa tccgcgtttc gggtagcggt gcgaggctgt ctgtcaggtg actaatggca
    19621 tcatcaagcc gatcattaag cgcctgatgc atttgcgaaa tggcacgact gggaaaccac
    19681 agcacgccga ttaacgtcat cagcaaggtt ggatagaggc caacaacgat acaccacagc
    19741 gttaagcgca cgaccacttc cggatagtcg agcatggcgg ggaaggtttg cccgtaaata
    19801 gcgacaatgg cgacggcgaa aaagaccagc cccaagcgat gggtgcgcat caaaaacatg
    19861 cagcccatca ggatcggtcc ggcgatgatc aatcggatca acggttcgcc gtatgaccat
    19921 ttgtagatca aaaacaggct gccgatctcc agcaccgtgg caaccacaaa caagatcgcg
    19981 acaaatttgg tgtaaaacgc gttcgactga ataccgtaaa acagcactgc cagcgataac
    20041 gccacaaaag ggatctcaaa ggtcatcgag atcagaatca ccagcaggca gcctacccag
    20101 agttgcacgg tctgcggcac gcgacctggt cgccgctcgc ttaactcttc atgaaagaac
    20161 gccagcagcc tgaccaccgg taatggcagg gagttgagcg cgctcattat tgcggctcaa
    20221 gattagcgac tgccgaagcg ccgatgcgga acatttccgg gtcaggttta tcgaccatga
    20281 ttttgaccgg aaaacgctgg gcaacgcgga cccagttaat agaacgagac actttcggca
    20341 ggccgcccag caccaggccg ccgtcatccg gtagcacgcc gtagccaatc gaatccactt
    20401 taccctcgaa ggttttgccg ctgtcactca tcaggcgaat cgttgcgggt gtacctgagc
    20461 gaatattttt cagatcggtt tcgcggaagt tggcgatcac ataccagtga cgagtgtcga
    20521 ttagggtaaa aatagggcgc atggcagaag caaattgccc gacggaggtt ttgagggaaa
    20581 tgacccggcc atcaaacggc gcgcgaacgg tcgccatttc cagatgcagt ttggtcaggg
    20641 caatatccgc ttcgaccgcc gcacgctggg caactaatgc atccacgccg ctgacggcgc
    20701 tggcggctga ctgcgcctgt aacaatacgg cattaagatc cgcttctgcg gcgcgctgcg
    20761 ccgttcttgc acggtcaaca tcttccgctg agacaaaacc ttctttcagt aatggctcgg
    20821 tgcggcgtaa tgtatctgtg gcctgtttcg cggcggcacg ggctttttct accgtggcat
    20881 taaccgagtc ggcaccaaac tgttgcgcgt caacgctacg ctgggtgagc ataatttgct
    20941 tatccagcgc cgcgagggag gcttcagctt tcgccagatt ggcttcgtac gggcgcgggt
    21001 cgatgcggaa cagcaaatcg ccctgtttga ctgcctggtt gtcggtgacc gccagttcta
    21061 caatgcggcc gctgacttcc ggtaccacat caatggtatc tgctgacgcg taagcgtcat
    21121 tagttgatgg cgcactgtct acgcgccaga taacgaaaac aagggcaacc agcgccaacg
    21181 caaccactaa cagagcaggg aatttactgc gaggagcttt tttcggcgta ctttccatga
    21241 gcgcgtctca aatagattag aaaaatgcca gccagaacag catggcgtag agagcaaaaa
    21301 gggcggtata aataattccg acaaatgcca gattgatatt tgcccgctga ataactcgcc
    21361 tcgtgatgag cgtcaaaata aggctggcaa tggcgcagaa aaaccagctg ggataataag
    21421 cgccgatcac cgggattgcc ggagataatg agcatcccga aaccatgaag acgggaatta
    21481 tccaggctgt ttttttgagt tgcatcgcca tgcgagagag aactgttggc atattaaata
    21541 catcctgtct gctgcgaaca atatctgtag tgaatatcaa tatccattga aggcgtcagt
    21601 atggctattt tcttgatgaa taaaaatagc tt
//
LOCUS       DAJOSY010000076        21504 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJOSY010000076 DAJOSY010000000
VERSION     DAJOSY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.076"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(680..2713)
                     /gene="betT"
                     /locus_tag="OHN27_002993"
     CDS             complement(680..2713)
                     /gene="betT"
                     /locus_tag="OHN27_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCQ2287391.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEGARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2842..3429
                     /gene="betI"
                     /locus_tag="OHN27_002994"
     CDS             2842..3429
                     /gene="betI"
                     /locus_tag="OHN27_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HCQ2287392.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFHRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            3443..4915
                     /gene="betB"
                     /locus_tag="OHN27_002995"
     CDS             3443..4915
                     /gene="betB"
                     /locus_tag="OHN27_002995"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCQ2287393.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4929..6599
                     /gene="betA"
                     /locus_tag="OHN27_002996"
     CDS             4929..6599
                     /gene="betA"
                     /locus_tag="OHN27_002996"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCQ2287394.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWQEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6812..7480
                     /locus_tag="OHN27_002997"
     CDS             6812..7480
                     /locus_tag="OHN27_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287395.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
                     DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQIPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(7723..8418)
                     /locus_tag="OHN27_002998"
     CDS             complement(7723..8418)
                     /locus_tag="OHN27_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCQ2287396.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(8411..9838)
                     /locus_tag="OHN27_002999"
     CDS             complement(8411..9838)
                     /locus_tag="OHN27_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCQ2287397.1"
                     /translation="MSIKTSNTDFKTRIRQRIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRDAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9849..10568)
                     /locus_tag="OHN27_003000"
     CDS             complement(9849..10568)
                     /locus_tag="OHN27_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCQ2287398.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(11095..11949)
                     /gene="rclR"
                     /locus_tag="OHN27_003001"
     CDS             complement(11095..11949)
                     /gene="rclR"
                     /locus_tag="OHN27_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCQ2287399.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            12175..13500
                     /locus_tag="OHN27_003002"
     CDS             12175..13500
                     /locus_tag="OHN27_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCQ2287400.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENASIAVNERGAIVV
                     DKRLHTTADNIWAVGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            13609..13845
                     /locus_tag="OHN27_003003"
     CDS             13609..13845
                     /locus_tag="OHN27_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2287401.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTALE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            13857..14450
                     /gene="rclC"
                     /locus_tag="OHN27_003004"
     CDS             13857..14450
                     /gene="rclC"
                     /locus_tag="OHN27_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCQ2287402.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(14609..14737)
                     /locus_tag="OHN27_003005"
                     /pseudo
     CDS             complement(14609..14737)
                     /locus_tag="OHN27_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            15040..15891
                     /locus_tag="OHN27_003006"
     CDS             15040..15891
                     /locus_tag="OHN27_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2287403.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECILINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCEYADMPKEWTTTRGLY
                     ASFRYEGNWENYPDWVRNIYLIELPARGLARVNGSDIERFYYNEDFVEKDGNDVVCEI
                     FIPVRPV"
     gene            complement(16031..20287)
                     /gene="fdeC"
                     /locus_tag="OHN27_003007"
     CDS             complement(16031..20287)
                     /gene="fdeC"
                     /locus_tag="OHN27_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HCQ2287404.1"
                     /translation="MSHYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSSDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNTISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKMTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQAILEVDGSTPKVANDNDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSDVTLTASSENLVLDPKGTAKTNEQGQA
                     VFTGSTTIAATYTLTAKVEQANGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATMTVTLMAGVNPVGGSMWVDIEAPEGVTEKDYQFLPSKADHFSGGKITRTFSTSK
                     PGVYTFTFNALTYGGYEMTPVKVTINAVAAETENGEEEMP"
     gene            21402..21503
                     /locus_tag="OHN27_003008"
     CDS             21402..21503
                     /locus_tag="OHN27_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287405.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
BASE COUNT         5601 a         5472 c         4983 g         5448 t
ORIGIN      
        1 tcaaactcag gagataatca cgcaagaatg caatattaaa attcagatac tcatttttgg
       61 taataaagaa gtagttggac tatttcgata tttataattt cccactacgt attgatgccc
      121 actcatgagt aaaggcgcat tatatgaatt taacaatcca gatcaactga aaatacctca
      181 acctcataaa cacatagcgt caacattcaa tgacataatg agtaaagatg ttggttatgc
      241 atacacgtca ttactctatg cctatccctt aacaacctgc cttttaagac tgaatccatt
      301 cagcaataga tagccaacat tataacaatg acaataaaaa caatatttaa aacaatcaat
      361 aaaatatcag acacagacaa ttaaacctta aacaacaaac ggttacattg attttagttg
      421 atttgcgcta cgcgttcccc atgaataaag cgtcatttca aattagcacc aatatttaca
      481 ttaaataaga ctaagcttat tgaacattca caacagtcca aatgtaggtt ttgggcttca
      541 atagttcttt tcttttttat tatctgttgc ctggcaatca tcaatcgata ctaagggctt
      601 aaccgtacag atataacgta tcaagaggta agagaaaccg cgccatatgc aaacatggcg
      661 cggttttatg caataacaat cacgcgtccg ggaacatcac gctatggccc ggcgcttcac
      721 gatggagatg aataaagtta aggtgccgct cgtactggtc aagaatatcg gtgatcacct
      781 gctctttgct gtagtccatc aggtcgttgc cctggctgcc ttctaacagg aaggtttcca
      841 gccggtagta ggtcgattta ccgcttcgtg cgcggtaggt aaagcccggc accgaatatt
      901 gctgcggcca aatctgatag acaaagtttt gctcttcgcc catatgcacc aacaaatcca
      961 gatgccccaa ctgctgtccc tcttccggtg gcaggctttt tagctccacg tacgcaccgc
     1021 gcaaccgcaa ctcctgcgcc acttcttcca ttgccgggta acagaccgtc tccatcatct
     1081 gtttagtgta acgcgtgccc ggataattca tcaggcgcga gagacgtttt ttccagctca
     1141 ggcgatcctg aagccccagc ggtcgcggtg cggtatcgcg gttggcactt tcacggcggt
     1201 aatcttctac cttcagagat ttatacaacc ccgccatcac gaagaagatc acaaagctga
     1261 acggcagccc cataatcacc gtggtgtttt gcagcgcgga tatcccgttg gtcatcagca
     1321 tgccgagcgt cagcaggcca atcgccaccg accagaagac gcgcagccag ccgggggcgt
     1381 cgctgttgat atctttaagc tgcgaggtga aattccccag caccagcgcc ccggagtccg
     1441 ccgaggtcac ataaaacagc aggccagtaa tggtggcgac ggaggcgcta aaggtaaacg
     1501 ccggatactg cgccagcagg ctgtagaagc cgcgctccgg atggaccatc gcttcctcgg
     1561 caaatgccgc tccgccgtgg atgatttcat acagcgcgct attgccgaac accgagagcc
     1621 ataacagcgt gaaggtaaac ggaataatca acgtgcccag cacgaactgg cgaatggtac
     1681 gcccacgcga gatacgcgcc aggaacaagc caacaaacgg cgaccatgcc acccaccatg
     1741 cccagaagaa gagcgtccag ttattcatcc actcaactgg acggtcgaag gcaaaactgt
     1801 tgagcgtcat gcccataaag cgattcacat agtcgccaac attcagcacc agtgcattaa
     1861 gcaggaacga agtgtcgccc ataaacaaca cgaacaggat caatcccaac gccagcgcga
     1921 cattaagctc cgataacaca cgaatgccct tatcgacacc ggatgtcacc gagatagtgg
     1981 cgattatcac cgacaaggcg atcagtgccg cttttgccgc catcgaatcg ggaatatcaa
     2041 acagtacgct caagccatag ttaagctgca ccacaccgat accgagcgta gtggcaatac
     2101 cgaagatagt gccgatcacc gccgcgatat ccactgagtg acctatcggc ccgttaatcc
     2161 gtttaccgaa gatcgggtac agcgccgagc ggatggtgag cggcaaatta taacgatagc
     2221 taaagtatcc gagcgccatg cccatcagcg catacatcga ccagccggtt aagccgtagt
     2281 gaaacagcgt ccagaccatc gcctgacgag cgccctcaat cgtctgtccc gcgccttccg
     2341 gcggctgcat atactgcgtt accggttcgg ctacggaaaa gaacatcaga tcgataccga
     2401 tcccggcagc aaacagcatc gccgcccaac tcagcaggct gaattccggt ttggattgtt
     2461 ctggcccgag cttcaccgaa ccaaaacgcg aacaagcgat acagaccaca aagacaatat
     2521 agagcgttgc cgccagcaga tagtaccaac cgaaggtttt agaaacccag tccagcgtgc
     2581 ggccaatcca cagggccgag aagtcgcgaa acaggatcgt tgtcagggaa aacaacaaaa
     2641 tcagtccggc ggaggtgtaa aacaccaccg gattgatttt gtccttttcc ctgctgtgtg
     2701 aaaggtctgt catccagtat ccccactgtt attgttacca tttaaaatca aattcgtaac
     2761 aattaagaca cattttatat tgaacgtcca atcaataacc gctttaatag ataaacaacg
     2821 ctgatgaatg gagtggcgaa aatgcccaaa ttggggatgc agtcgatccg gcgcagacaa
     2881 ctgatcgacg ccacgctgga agcaataaat gaagtgggca tgcacgatgc aacgatcgcg
     2941 cagatcgccc gccgtgcagg cgtttctacg gggatcatca gccactattt cagggacaaa
     3001 aatggtctgc tggaagcaac catgcgcgat atcaccagtc agctgcgtga cgcggttttg
     3061 aatcgattac atgcacttcc gcagggcagt gcagagcagc gattacaggc gattgttggc
     3121 ggaaacttcg atgaaacgca ggtgagcagt gcggcgatga aagcctggct ggcgttctgg
     3181 gccagcagta tgcatcagcc gatgctctat cgcttgcagc aggtcagtag ccgccgctta
     3241 ttgtcgaatc tggtgagtga atttcaccgc gagttaccgc gccaacaggc acaggaagcg
     3301 ggctacggcc ttgccgcgct gattgacgga ttatggctgc gcgcggcatt gagcggcaaa
     3361 ccgctggata aacctctcgc acactcgctg acccgtcact ttattactca gcatttaccc
     3421 accgattaac cgaggagacg tgatgtcccg aatggcagaa cagcagcttt atatacatgg
     3481 tggttatacc tccgccacca gcggtcgcac cttcgagacc attaacccgg ccaacggtaa
     3541 cgtgctggcg accgtgcagg ccgccgggcg cgaggatgtc gatcgcgcag tgaagagtgc
     3601 ccaacagggg caaaaaatct gggcggcgat gaccgcgatg gagcgctcgc gcattctgcg
     3661 tcgggccgtc gatattctgc gtgaacgcaa tgacgaactc gcaaaactgg aaaccctcga
     3721 caccggaaaa gcatattcgg aaacctcaac cgtcgatatc gttaccggtg cggacgtgct
     3781 ggagtactac gccgggctga tcccggcgct ggaaggcagc cagatcccgt tgcgtgagac
     3841 gtcatttgtt tatacccgcc gcgaaccgct gggtgtggtg gcggggattg gcgcatggaa
     3901 ctacccgatc cagattgccc tgtggaaatc cgccccggcg ctggcggcag gcaacgcaat
     3961 gattttcaaa ccgagcgaag tcaccccgct taccgcgtta aagctggctg aaatttacag
     4021 cgaagcgggc ctgccggacg gcgtatttaa cgtgttgccg ggggtaggcg cggagaccgg
     4081 gcaatatctg accgagcatc cgggcattgc caaagtgtca tttaccggcg gtgtcgccag
     4141 cggcaaaaaa gtgatggcca actcggcggc ctcttccctg aaagaagtga ccatggaact
     4201 gggcggtaaa tcaccgctga tcgttttcga tgatgcggat ctcgatctcg ccgccgatat
     4261 cgccatgatg gcaaacttct tcagctccgg tcaggtgtgt accaatggca cccgcgtctt
     4321 cgttccggcg aaatgcaaag ccgcatttga gcagaaaatt ctggcgcgcg ttgagcgcat
     4381 tcgcgcgggc gacgttttcg atccgcaaac taacttcggc ccgctggtca gcttcccgca
     4441 tcgcgataac gtgctgcgct atatcgccaa aggcaaagag gaaggcgcgc gcgtactgtg
     4501 cggcggcgat gtactgaaag gcgatggctt cgataacggc gcatgggttg caccgaccgt
     4561 gttcaccgat tgccgcgacg aaatgaccat cgtgcgcgaa gagatcttcg gtccggtgat
     4621 gtcgcttctc acttatgaat ctgaggacga agtgattcgc cgcgccaatg ataccgacta
     4681 cggcctggcg gcgggcatcg tgacggcgga cctgaaccgc gcgcatcgcg tcattcatca
     4741 gctggaagcg ggtatttgct ggatcaacac ctggggcgaa tccccggcag agatgcccgt
     4801 tggcggctac aaacactccg gcattggtcg cgagaacggc gtgatgacgc tccagagtta
     4861 cacccaggtg aagtccatcc aggttgagat ggctaaattc cagtccatat tctaaccagg
     4921 aggtttattt gcaatttgac tacatcatta ttggtgccgg ctcagccggc aacgttctcg
     4981 ctacccgtct gactgaagat ccgaatacct ccgtgctgct gcttgaagcg ggcggcccgg
     5041 actatcgctt tgacttccgc acccagatgc ccgctgccct ggcattcccg ctacagggta
     5101 aacgctacaa ctgggcctat gaaacggaac ctgaaccgtt tatgaataac cgccgcatgg
     5161 agtgcggacg cggtaaaggt ctgggtggat cgtcgctgat caacggcatg tgctacatcc
     5221 gtggcaatgc gctggatctc gataactggg cgcaagaacc cggtctggag aactggagtt
     5281 atctcgactg cctgccctac taccgcaagg ccgagactcg cgatgtgggc gagaacgact
     5341 accacggtgg cgatggtccg gtgagcgtca ctacctccaa acccggcgtc aatccgctgt
     5401 ttgaagcgat gattgaagcg ggcgtgcagg cgggctaccc gcgcacggac gatctcaacg
     5461 gttatcagca ggaaggtttt ggtccgatgg atcgcaccgt cacgccgcag ggccgtcgcg
     5521 ccagtaccgc gcgcggttat ctcgatcagg ccaaatcgcg ccctaacctg accattcgta
     5581 ctcacgctat gaccgatcac atcatttttg acggcaaacg cgcggtgggc gtcgagtggc
     5641 aggaaggcga cagcaccatt ccaacccgcg cgacagcgaa taaagaggtg ctgttatgtg
     5701 caggtgcgat cgcctctccg cagatcctgc aacgttccgg cgtgggtagc gctgaactgc
     5761 tggcggagtt tgatattccg ctggtgcatg atttacccgg cgttggcgaa aatcttcagg
     5821 atcacctgga gatgtacctg caatatgagt gcaaagaacc ggtttccctc taccctgccc
     5881 tgcagtggtg gaaccagccg aaaatcggtg cggagtggct gtttggcggc actggcgttg
     5941 gtgccagcaa ccactttgaa gcaggcggat ttattcgcag ccgagaggaa tttgcgtggc
     6001 cgaatattca gtaccatttc ctgccagtag cgattaacta taacggctct aatgcggtga
     6061 aggagcacgg tttccagtgc cacgtcggct caatgcgctc gccaagccgt gggcatgtgc
     6121 ggattaaatc ccgcgacccg caccagcatc cggcaattct gtttaactac atgtcacacg
     6181 agcaggactg gcaggagttc cgcgacgcaa ttcgcatcac ccgggagatc atgcatcaac
     6241 cggcgctgga tcagtatcgt ggccgcgaaa tcagccccgg cacggaatgt cagacggatg
     6301 agcagctcga tgagttcgtg cgtaatcacg ccgaaaccgc cttccatccg tgcggtacct
     6361 gcaaaatggg ttacgacgag atgtccgtgg ttgacggcga aggccgcgtg catgggctgg
     6421 aaggcctgcg cgtggtggat gcgtcaatta tgccgcagat tatcaccggg aatttgaacg
     6481 ccacgacaat tatgattggc gagaaaatag cggatatgat tcgtggacag gaagcgctgc
     6541 cgaggagcac ggcgggatat tttgtggcaa atgggatgcc ggtgagagcg aaaaaatgag
     6601 tcgtgatgtg aactaacgca ggaaccgatc agattgagag ttaccgttcc agagaggggg
     6661 accgaatcct tatataaaca ctgaggtaac tctcatgctt catatccagt attcatgatg
     6721 ctggtttttt gctgatattt accccgaaca ttcctgatac agactattaa tacaacctta
     6781 tctgcgacag attttatttt ctggatatat tatgcgtgaa caaatcaaac aggatatcga
     6841 tctgattgag attttatttt atctgaagaa aaagattcgt gttatccttt ttattatggc
     6901 tatatgtatg gctatggtgc tgttgtttct gtatatcaat aaagacaata taaaagtgat
     6961 ttacagccta aaaataaacc agacaacgcc aggtatactt gttagctgtg atagcaataa
     7021 taattttgcc tgtcagacca caatgactga agatgttata cagcgaatta ctacattttt
     7081 tcacaccagc ccagatgtca agaacagaga aataaagctg gaatggtcag gagataagag
     7141 agatttacca actgctgaag cggaaatatc tcgcgtgcag gcgtctatta tcaaatggta
     7201 tgcgtcagaa taccataatg gcaggcaagt tctcgatgag atacaaatac cttcagcaat
     7261 taacagtgag ctttatacaa aaatgatata cctgaccagg aactggtcat tgtatccgaa
     7321 cggtgatggc tgtgtaacta taagctcacc agaaataaaa aataaatacc ctgctgccat
     7381 ttgcctggct ctgggatttt ttctaagcat tgtaatttct gtaatgtttt gccttgtcaa
     7441 aaaaatggta gatgaatacc aacaaaactc tgggcagtaa taaacaacat tctgatagag
     7501 agtgcctgta taaagctgaa gtgacaatga ccgggaaggg aacccgtttt caggaatgga
     7561 tgcctgtctt tttcactgta aatatcctgg cattggtaat accaggatat ttacagaaga
     7621 gcagcataac catgacggaa cgggattaag tcaggttgtc ctaaacgcta ttttcaacct
     7681 cgtatgcctt cttcaggttt atgtccagac ttcatacctc tctcagcaat cctcaataat
     7741 cagatacacc gctttcaccg gaccatgaac cccgacgact ttgataagct caatgtccgc
     7801 cgttgagctg ggaccactaa tgatgttaat acatgacggc attcgttcac cggcctgcgc
     7861 tttctgatgc aatttttctg cgagttgcgc tacacgcggc aggattgtgc ttttacgcag
     7921 gataaaaaga gaagattccg ggagcaggct caatgaacgc ccgcgctcgg cggcggaaaa
     7981 aagaaccacg cctcccgatt cggttaaacc atattcagca tacacaacac ccactttagc
     8041 ctgctctgcc tgcgagatat tctcggcacc tttcgccgga tcccaaacaa cggcattgca
     8101 ttcctgctgc aaacgttcac taatccccaa ttcctccagc ctcgtgtcac cgctaatcac
     8161 gaccgactga tctcccagct ctttacacag acgtattgca gcttctgccg ccttcgcctc
     8221 gctggtcagc tcacagcgcg tcaacataac atcgctggca aactgaataa acgcgtcaca
     8281 gcgctgctgt tggttaagtt gggtaagccg ctcgttagca tagttgttaa gcggcgcatc
     8341 ttctgcttgc ggttcaagtc gcagcgggcg acccagtgcc tgagcaacgt tattcaaaaa
     8401 ttcgcctcga ttatccattc tttttctcct gcgcctgatg tttcttaaac caactacgga
     8461 aactctctcc gtcagcttca ggaagatcgc gtgcttccat ccagtcgcta atcgcgccaa
     8521 atttgagtgg tgttttgccg ccattgataa accagcttgc cgcatgagcg ccggccatca
     8581 tcccgacttt ccacaatcct ggatgactat tggcataagc gaacattttt atcgcccgtt
     8641 gctctgcttt tgcggtgatc cctttttcag ccatcacccg acgatgacgc aaaatcagtt
     8701 ttgacagcgg aatacgcacc ggacacacgc tgtcacaagc agtgcataaa gagcaggcgt
     8761 agggtaaatc tttaaaatct ttatagccgc caagtagcgg agaaatcacc gcaccaattg
     8821 gccctggata aatagagcca tatccatgac cgccaatatg gcgatatgcc ggacaagtat
     8881 tcatacaagc cccgcagcga atacagcgca gcacatcccg aaattcagag gccagcacct
     8941 cagaacgccc gttatcgaca ataaccagat gaaactcttc aggaccatca acgtgccccg
     9001 cttcgcgcgg tcctgtcagc caggtgttgt atcccgtcaa acgtgcacca acggcactgc
     9061 gcgccagcat ggtgatcaat acatctacct cggcaaacgt gggggcaata cgctccattc
     9121 ccatcactgc aatatgcgtt ttaggcagcg tggtacacat tcgcgcatta ccttcattgg
     9181 tcaccaggca taccgaacct gtctctgcca ccgcgaaatt acagccggta atacctattt
     9241 cagcactgag gaaatcttcg cggatttttt gccggatgaa taaggtcatc gcttcaggcg
     9301 tttccggccc ctcatagccc agacgttcgt gtagcactcg acggatctga tggcgatctt
     9361 tatgaattgc cgggaccaca acatgagatg gcggatcttg atccagctgg agaatatatt
     9421 cacccagatc ggtttcaatc acctgaatgc cagcatcctg caacacatga ttgacaccaa
     9481 tctcttcggt caccatcgat ttagatttca ccaccttccg ggcatttttg cgttgggcaa
     9541 cctgtaaaat gtagcgggta gcgtcttctt tggtttttgc aaaccagacg tgaccgccgt
     9601 tttgcgtcac tttttctgag agctggtaca gataagcgtc gagattactc agtacatgat
     9661 cacgtatctg ggcgtcccga tcgcgccact cctcccagtg ccccaattca tcgaccattt
     9721 tttgccgatt tgccccaata cgctgctgcg cgtttgccac cgctttgcgc atgatcggat
     9781 cttcaattcg ctgacggatg cgtgtcttaa aatctgtatt actggttttg atcgacatct
     9841 ttatatcctc agcggctcat caacacttca gcaatatgca tcactttgac tttctgccct
     9901 tcccgttgta atcgcccact gatgttcagc aggcaactca cgtcagcacc aattaaatac
     9961 tcagggcgga cttccatcag gtgcacaacc ttttctttca ccatctcgcc ggatatttcg
    10021 gccattttga ccgagaacgt gccgccaaat ccgcagcagg tatcctgttc agcaaaggtc
    10081 aacagctcca gtccacgcac atttttcagc agcgtaagtg gctcgtcctt cactcccagc
    10141 ttacgggcca ggctacaaga tgggtgatac acagctctcc cttgcaaact ggcacctaca
    10201 tcgactaccc ctaatttatt aacaataaaa gaggtgagat cctgcatacg cgcggcaacc
    10261 ttttcggcac gtgatgccca ttcaggttca tccgccagat acgtcgggta actttttacg
    10321 gcataggtgc aagagccagc cggtgaaata atgggatcgt cgttatcctc cagtgcggcg
    10381 atcagatttt tcatccctgg aatcgcttct ttgatataac cgctattgat cgcaggctga
    10441 ccgcagcatc cctgtttctc cgggaaattt acgcgacagc cgagtttttc cagtagcagc
    10501 acggagtctc gtgccattct tgatttcagg gcgtcaccaa tacaggtgac aaagaaattg
    10561 acattcacaa ctaatactcc attacttcat gcccatttat gcccgatgtt tcccccccac
    10621 cgggggatca atcatcaata aatatggcgt tcttttttaa ttggctagca acataccacc
    10681 cctcatttgt atgacaaatg ataatctttc atccaaacgt caaatgcaat aactaatttt
    10741 atttctttcg gcatcgctca cattttatgg ggaaatcgac gatcattttt ggcataagaa
    10801 atccatttaa aaatggctac atcccttata cctattcaga gctacttatt caggggttat
    10861 tgagatatac actgatggtg tacaaaaaaa tcaccgacaa taatcacgta tcaagtaata
    10921 gtcgggattt tatttaattg aagcaattta gtttgcattt ttatttaaaa actgagctag
    10981 agtccatcag aaaaaatata taaagcccaa accaccgttg gaaatagaaa tagttgaaag
    11041 gctcacaact cccggaatgc ggattatctt tgccctgatt tctggcggtt ttattcaggg
    11101 tgcaagctgt ctgacccttt cccgatattc tcccggggta caaccaaact cgcggacaaa
    11161 cgccttgtga aaagatgatt cactggcata gcctactgac tcagcgatca ccacaacagg
    11221 gagcgtttcc cgggaaaaca tctgggccgc tatttgtaga cgcaactttg ttaatacagc
    11281 cagcggcgtg gttccggaaa catcacggaa aagctgggca aaacttgccc gggacatgtg
    11341 agcgatgctg gccagcgatt cgacggtcca ggcgtgtcct ggcatttcca gcatttgctg
    11401 tattaccgca ccaagacgtg gatgcagaag caaactgaga atgtttttct cagtattaac
    11461 ctgtgcaatc cactcacgca ccgcgagggt aaagaatgta gcgcagatct ggctacacag
    11521 tgcatccact cccggcattg ccgatctgga ttcctgttgt aaaaacggga tcgcctccct
    11581 cagccagtta tattccacgc tgtggttaac cggtgctaaa aacagcgttt ccggcaaaga
    11641 cgttaaaaaa taacgcgccg aatgttgcaa ccgaagagtg ccgcagacaa tacaggtcga
    11701 ttcattatcg acatgactca ggcgatgagc tgaattttgt ggtagcagga ccacatttcc
    11761 cgggcgtaat gtaaaaatct cccccgtcgg catttccagc ttcgccgctc cttgcgttaa
    11821 cgcatgccaa cgaataaccg ataattcccc ggcaccatgt ggaagctgcc agtcacttcc
    11881 taacacgcaa ttcttatcga tcgttccttg tggagcgtta agcatcaaca aacggctaag
    11941 ggcatccata tcagactcct gagtggtttt tgattacaaa caccaacaat cttagtaaat
    12001 aattaatgta ataccatcaa atagttaatc atgatttttg ttttgtgatt ctgattaaga
    12061 aaaagaaaat accagacgat aagacaaaat atcaagcgtt gtgaagaaat gttatttgct
    12121 cttttgcgtc tatagtcatg atgtcaaatg aacgcgtttc gacaggaaat catcatgaat
    12181 aaatatcagg cagttattat tggttttggc aaggctggaa aaacattagc cgtcacgctg
    12241 gcaaaagcag gttggcgagt ggctctcatc gaacaatcaa atgcaatgta tggcgggacc
    12301 tgtattaata tcggctgcat cccaaccaaa acattggttc atgacgcaca gcagcacaca
    12361 gattttgtcc gtgccataca gcgtaaaaat gaagtggtta attttttacg taataagaat
    12421 tttcataatc ttgcggatat gcccaatatc gacgtgatcg acggccaggc ggagtttatc
    12481 aataatcata gcctgcgtgt tcatcggcct gagggaaatc tggaaattca tggcgagaaa
    12541 atttttatta ataccggtgc acaaaccgtg gttccgccaa ttcctggaat taccaccacg
    12601 cctggtgtat atgacagcac cggattactt aatctaaaag aattgcctgg gcatttaggt
    12661 attttgggcg gcggatatat tggcgttgag ttcgcctcta tgttcgctaa ttttggcagc
    12721 aaggtaacca ttttagaagc agcttcgctg tttttgcctc gggaagatcg ggatattgct
    12781 gataatatcg cgacgatttt acgcgatcag ggcgtcgata ttatcctcaa tgcccatgtg
    12841 gagcgaatca gtcaccatga aaatcaagtg caagtgcata gcgagcacgc ccaactggcg
    12901 gtggatgcac tgttaatagc ttccggtcgt caaccggcta ccgcttcgtt acatccagaa
    12961 aatgccagta tcgcagtaaa cgagcgcggg gcaattgtcg ttgacaagcg attacatacc
    13021 accgcagaca atatttgggc ggtgggagat gttaccggcg ggctgcaatt tacttacata
    13081 tcactggatg attaccgcat tgtacgtgat gagttactgg gtgaaggcaa acgtagtact
    13141 gatgatcgga aaaatgtgcc ttattccgta tttatgacac ctcccctgtc cagggttggt
    13201 atgacagaag aacaagccag agagagtggt gctgatattc aggtggtgac attgcctgta
    13261 gctgcaattc cgcgtgccag agtgatgaac gatacccgtg gggtattaaa agcgattgtt
    13321 gataataaaa cccaacgtat attaggggca tcactgctgt gtgttgactc ccacgagatg
    13381 attaatatag tgaaaatggt gatggatgcc gggctgcctt atagcatatt acgcgatcag
    13441 atatttactc atccgtcgat gagcgaatca ctcaatgatc tattttcatt agtcaaataa
    13501 actcaaaatc agacgccaga acaaatattc tggcgtctca gagaaaagaa tcttattaat
    13561 tccctgttac tctctaactt cttaatcact tcattgatgg tattttatat gtttaaaaaa
    13621 tctgttttgt ttgcaacact attatctggc gttatggcat tttccaccaa tgcagatgat
    13681 aaaataattc tgaaacatat cagcgtttcg tcagtatcag catcaccgac agctctggag
    13741 gatgccattg ctgatatagc cagaaaatat aatgcttcat cctggaaagt cacatcgatg
    13801 cgaattgata ataattcaac cgcaacagca gtattgtata aataaggatg ttcacaatgg
    13861 aaaaatacct gcacctgtta agtcggggag ataaaattgg cctgacattg attcgtctga
    13921 gtattgcaat tgtttttatg tggattgggt tattaaagtt tgtcccttac gaggcagaca
    13981 gcattacacc attcgtcgca aacagtccac taatgtcgtt cttttatgaa cacccggaag
    14041 actataaaca gtatctgact cacgaaggcg aatacaaacc agaagcaagg gcatggcaat
    14101 cggccaataa tacctatggt ttttccaacg gtcttggcgt cgtggaggtg attattgctc
    14161 tgctggtttt ggctaatcct gtcaatcgct ggttaggttt attgggaggg ctgatggcat
    14221 ttaccacacc gttggtaaca ctctcatttt taatcaccac cccggaggca tgggtacccg
    14281 cattgggtga cgctcatcat ggtttccctt atttatccgg tgctggtcgc ctggtattga
    14341 aagatactct gatgctggca ggtgcagtca tgataatggc agattcggcg cgggaaattc
    14401 ttaaacaacg cagtaatgaa tccagttcaa cgttaaaaac tgaatattga taatccgaac
    14461 actgtctgtt gttcactttt tcaggcgtca ggcccccttt ctgatgatga agcctgacgc
    14521 catattaccg gagatatgac agagtgaaga tcaatatcac tccctgttaa aaggtttttg
    14581 tcgtcttagg taaaatacat gattaatatc aatcatgtat ttttacagca agacaaccac
    14641 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
    14701 taatttgtgc catttcttta tctgtcagtg agaaatcgat ttttgatggg catctgcaat
    14761 actctgaagc agactatacg gaaaattcca ctgtcagtcc ctccattagg catgaacaat
    14821 gagtctacgt taaaacgtaa cctcaaagta gtatgtggat tttgatatca cttatgcaaa
    14881 aaattcatta ataatgtagg actgaaacct ctctattttc ggggacaacg aagcagacgc
    14941 taccagtgct tttgccttcg cccttgctat ttttgataca cttagggccc agggtataac
    15001 gaaaatgtgc gatatgacaa agaattaacg gagaatgaga tgatcaggca gaagattcta
    15061 cagcagctcc tggagtggat tgagtgcaat cttgagcacc ctatttcaat cgaagatatc
    15121 gcacagaaat ctggctacag cagacgcaac atccagcttc tgttccgaaa tttcatgcat
    15181 gtgcctttgg gagaatacat tcgcaaacga aggctttgtc gtgccgccat tcttgtccgg
    15241 ctcaccgcga aatctatgct tgatattgca ctctctttgc attttgattc acagcagtca
    15301 ttcagccgcg aatttaaaaa gttattcggc tgctctcccc gtgaataccg ccaccgtgat
    15361 tattgggatc tcgcaaatat cttcccttct tttttaatac gtcaacagca aaagacggag
    15421 tgtatattaa tcaactttcc tgagacacct atttttggca actcatttaa atatgacatt
    15481 gaagtgtcga ataaatcacc ggatgaagaa gtcaaactac gacgtcatca tttagccaga
    15541 tgtatgaaga attttaagac ggatatctat ttcgtttcca cgtttgaacc gtcaacaaaa
    15601 tcggtcgatt tgctcacggt tgaaactttt gctggtacgg tatgtgaata tgctgacatg
    15661 ccaaaagagt ggacaacgac ccgaggactt tatgcctctt tccgttatga aggaaactgg
    15721 gaaaattatc ctgactgggt gcgtaacatc tatctgatag agttacctgc cagggggtta
    15781 gccagagtga acggcagcga tattgagcgc ttttattaca atgaagattt cgtagaaaag
    15841 gatggcaatg atgttgtttg cgaaattttt attcccgttc gtccggttta gttggtcact
    15901 atctcttatt gagttttatc cttgcctgat actatttaat cagtatcagg caaggtattc
    15961 agtgaataat ggcgttgaat atttcaacac cattattctt tattagatcg taactttcat
    16021 actattcaaa ttatggcatc tcctcctcgc cattttcagt ctctgcagca acggcgttaa
    16081 ttgtcacctt cacaggcgtc atttcgtacc cgccatacgt cagtgcgttg aatgtgaacg
    16141 tatagacacc tggcttgctg gtactaaatg tacgcgtgat tttcccacct gagaaatggt
    16201 cagccttcga cggcaggaat tgataatcct tctccgtcac tccttccgga gcctcaatgt
    16261 cgacccacat acttcctcct acgggattga ctcccgccat cagggtaacc gtcattgttg
    16321 cagtagtctt cccgtccgcc accagagaat ctacacgttc tggagatgcg gcgagcaccg
    16381 cgtttttatc atccgcgacg aatttagatt cagcagtttt cgtcgatacc tgaccgttgg
    16441 cctgttccac tttcgccgtg agtgtatatg tcgctgcgat agtggtagag ccggtgaaaa
    16501 cagcctgtcc ttgctcatta gttttcgccg tccctttagg atccagaact aaattttccg
    16561 agctggcagt cagcgtcacg tcgctatcct tcaacaggtt gttattggca tcagtcaccg
    16621 taactttata cgtctgtttg gttttgccat ccgccactgc acggttgcca atcacctcaa
    16681 tactggagat ggtcgccgta gtcttatcgg ccacaaacgt tacagactgc gcattcgaag
    16741 gctggtcatt tcctgccgac gccgtgatct catacgttcc ggcagtcacg gagaccactt
    16801 tcagttccgc tttaccctcc ttgtcggcgt tcaccatgat attaccgtct gcaagcggtt
    16861 tgacgccccg aggcagatta aagacgacca cagcgccagg cagaaggttg ccgtattgat
    16921 ccttaaccgt tgccgtcagc gtaaaggcat cattgtcgtt tgccactttt ggcgtgctgc
    16981 catcaacctc caggatcgcc tgaccggtac tgaaatccgc cttgaatttc accgtaaccg
    17041 tcttctgagc attattcact gaggccgtga tgctgtgttc ccccgcaacc gttgacataa
    17101 gctcaatgtc cactttccct gccgcattgg ttgttactgt attccccgtc ttgctggtca
    17161 caccctgcgg taaagtcagc gtaacttctt gcccctgcaa cgggttacca tagctgtcca
    17221 cgacggtcag ggttatctgg ttagcagact gtccattagc cagttggtta ttaaccttca
    17281 ctgtcatatc acgaatctca gccttagatg cgtcacctgc aacgttcagc accagtggct
    17341 gagcaacggc attttggccg ttcactcgcg gcatcacaga caactgaccc gcggcagatc
    17401 ccagcgttaa ggtcgccacg tagaccccat tacctttctc tgtccagttt cctgctgaag
    17461 gacgctcact ccccgtgctg gcggcaccgc taaacactgg tgcatctggc ttcagcccgg
    17521 tgaccgggtt cccgtacgca tccttcacgg tgaaggtgag ttccgtcgtc attttgacgg
    17581 tcggagcctt attgtccgca acaagcgtag agttcgctga tgacatctct ccggcgatga
    17641 ccgtcaactg cgcggcttcg gtggctgctg gctggccgtt gaagagcggc atgacacgaa
    17701 gctcgcccgt ctttccgcct gtagttaacg tagcaacata ggaaccgtca cctttttcgg
    17761 tccaactgga aacggtcgcc ccttcagagg cggtccccgt caaacttgcc gacaacgaaa
    17821 gaccactgat agtgttgcca tgtgcatctt ttgcaataag cgtcacggtt gtgctttcgc
    17881 cggcttttac gtgatcttct gcgacagaga ctttcgactg gtttgaagat aacgcatcag
    17941 ccaccacggt tacttttgcc gcatttgctg ccgcatcctg tccatttagc ttcggcataa
    18001 cttctaattc acccgccgta gagccgagag taatctgcgc agtccacgtc ccatcaccat
    18061 tatttgtcca gccagatgcc gtagaaccta cagcagcggc acccgctaat gacggcgctt
    18121 ccggcgtgag gctggtcaca gggttgtcat aggcatcttt tgccgtccag attgccgtaa
    18181 ctgtaccgcc aaccaccggt ttatcaggat tcagggtgat ggacgaatgt gctgcatcaa
    18241 gcggaccggc aacaaacttc agcgtttgtt gtaatgtgcc cagctgatac tgctcgctga
    18301 aagcacgcac aacaacgttt ccggcacggg tcgaagaaac cgtggcgctg taaacgcctg
    18361 gttttatttc cgaaatggca ccaacggtta caccattagt gtcttgcgga acaaatcgca
    18421 agcggctggc ttctcccgtc accggattac cctcggagtc caccgcagtc aacgtcaacg
    18481 tataggcttg ctgaccatca gcaaccacat cacttgacgg ctcgttagcg ctcagggtgg
    18541 agtttgccac atccatcatc gtggcccgca gttctgcagt gacggttttg ctcatgccat
    18601 caacgctaac agtaatcgtt gcctcacctg actgcgttcc ggtagtaaag acagactgat
    18661 acacccctgc ttcagtttcg gtgaactcac ccagtgttgg ctttgcctgt gatttagtgg
    18721 ccttcaggga acgagtcaca atatttccag ccggtttgaa ggctagttca gtcttgatct
    18781 gatctttcat gcccgtgact ggctgcccct cggcgtcgcg cagagacagc accagcggtc
    18841 tttgctcatt accgttagca agcatttgaa tacggctctg accgtcaagc gttaacgccg
    18901 tgcgatcggc gctcatacct gctccggtaa tgaccacctc tgtctgcacg cgttttgagg
    18961 cattgccttt gttatcgtag gcaaccgcag aaatcgcata ataattgtct ttgcctggac
    19021 gataagccgg gagcgttact tgccactgac taccctgacc ggtaattttg ccaccctcag
    19081 ccagtaatga cggcgcttcc cactgcacat ttttcagtcc gtgagttgct ttgctgacca
    19141 caagccccag ggaaagtgtc tgaccaccct taccttcaat acgttcaggc agagcaatac
    19201 ggatcacttc agatttgcgg tactcaagaa cgatgttgtt attacgctca accaggtcat
    19261 agcggctgcc tgccagtacc cgacgctcgc gaatgctgtc tgtatcgagt tgtttcgcca
    19321 gaggttcgcc aatccgataa ttaacttcca ggccaaagcg agtgtcattc tcaccactct
    19381 tgccctgctt atgcccggcg ctcagtgtca gaagaggcac tggcgtatag gtcacctcgg
    19441 cagaaatagc atgcgggtct ttctggcgct tatctttacc aaacagcccg acttcatcgc
    19501 cataatactg ttcatacatc aggcttgcgc caagctgcgg ccaggcgggt aaatagccct
    19561 cagcacgaat atcccagcca ttcgccgggc gttcctgata atcctcaaca tccggcgatt
    19621 ttttccagcc agaagcccgg atataaccat tggcgctcag tttcagataa tcgcgccagt
    19681 attccgcacc aacaccaatg cgggtatgac tacgggataa atcatggtcg ataaaggtgt
    19741 tcacccccgc catccagtca tttcctgaaa aatgacgcca gccaaaacca atatttgact
    19801 gagtacgatc gtcggtacga tgtattgctc cctgagtgaa caacatattt gttggcgtat
    19861 cataaatcgg ataaagcatt tccagcgaag aatccttcag cgagaaatct ttatcgacat
    19921 tcagtttgac gcgcgcagta ccatatttcc cgagccactc ctgtatttcc tggttagctt
    19981 tagctgtggc cattccggta ataaagttac gtgtcgcatc gctatctggc tgactgctta
    20041 aaaatgtccc ggcatttgcg gcaaacgacg cgacattttt ctccacgtta ttatcagcag
    20101 ttaccgtagt atttcccatg ctcaaccgtg gctgaaccgc atgctgcgca cgtgccgcca
    20161 ttactggggt aaaggtgaca gcgagtggaa aaagaacctg aacagagata tttgcccacg
    20221 ccacgcagcg ggccagaact gaataacgaa atcgtggttg tttatgacct gttttataat
    20281 gtgacattga gataatttcc tgatgaacga attaacgaac ataggcagac acaaataatg
    20341 gcgtctgctt gtcagaaaaa ccggggattt cccgatttcc ttttccgcag aaaaggaaat
    20401 acctttatgt aagaaagtgc accgccacct aatatatcca gtgcctgtag ccactcatca
    20461 ggtggttggg cgcgggatta tatttttatt tatgggggaa aatttagctt tttgtgcaag
    20521 tcattaaata gatacctaag ttatatttct tactctgcgg acagtgaata attcactatt
    20581 cagagtgaat aacaatgacc tgcctttaat taaaactatt ggaaatacgt cgaaataggt
    20641 atatatgcca ggataaacca atatattttt tatggatatt cattttcctt catcggactg
    20701 taaattgcgg agatatcgaa cttgcactac ccatcgcaac actcaaacag atttatgaat
    20761 ttcgttcata acccctgaca aattctgccg tctaatcgag cttttccctt tacgttatgc
    20821 ttctcatgaa cgataacaca acttgttcat gaattaacca ttccggataa actatgggat
    20881 caaattcagg catcgaaagg aactggctgg tttccgttaa tggattaaat atctattatt
    20941 ctcccgagag taaaactatc aaatcaatga gtaaccgata cagcctcaca aattctttca
    21001 gataattaac ccgtcactgc ggcacgttct ctcataaggc gtgcctgcag tgtaatccag
    21061 tatgtatccg gcatagttga cggtttaata gaactcaatt cctctcccag gtcaccaaca
    21121 acgatatctt gtataacacc ccaggagata cgacattatg atgagatgcg atatgctcct
    21181 cagcaccaat caacgcccgg actgggccag tcttgagatc agactcattc ccggaactga
    21241 cttcaggcga catatatcaa tgacgttggt gcaataaaag atagttgatg taatacatct
    21301 attcaaattt gtgatacatg tcaaaaaatt ggttgaccaa actcgttatt tatataaggg
    21361 cacttacgaa gtgcactctt ttttaaagcg aggaagtacc aatgaaagag aataaagtac
    21421 agcaaatcag tcataaactg attaatatcg ttgtttttgt cgcaattgta gaatacgcct
    21481 atttatttct ccatttctat taat
//
LOCUS       DAJOSY010000077        21081 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJOSY010000077 DAJOSY010000000
VERSION     DAJOSY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.077"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            58..660
                     /locus_tag="OHN27_003009"
     CDS             58..660
                     /locus_tag="OHN27_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCQ2287406.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLCTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(699..1124)
                     /gene="nudI"
                     /locus_tag="OHN27_003010"
     CDS             complement(699..1124)
                     /gene="nudI"
                     /locus_tag="OHN27_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCQ2287407.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1403..1945
                     /locus_tag="OHN27_003011"
     CDS             1403..1945
                     /locus_tag="OHN27_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287408.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2045..3247
                     /locus_tag="OHN27_003012"
     CDS             2045..3247
                     /locus_tag="OHN27_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCQ2287409.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3467..4249
                     /locus_tag="OHN27_003013"
     CDS             3467..4249
                     /locus_tag="OHN27_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCQ2287410.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4264..5469
                     /gene="rhmD"
                     /locus_tag="OHN27_003014"
     CDS             4264..5469
                     /gene="rhmD"
                     /locus_tag="OHN27_003014"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCQ2287411.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVTDMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLLETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5526..6823
                     /locus_tag="OHN27_003015"
                     /pseudo
     CDS             5526..6823
                     /locus_tag="OHN27_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6832..7635
                     /gene="yfaU"
                     /locus_tag="OHN27_003016"
                     /pseudo
     CDS             6832..7635
                     /gene="yfaU"
                     /locus_tag="OHN27_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
     gene            complement(7676..8578)
                     /locus_tag="OHN27_003017"
     CDS             complement(7676..8578)
                     /locus_tag="OHN27_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCQ2287412.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDEPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAAIQLSEADLAANNH"
     gene            complement(8772..9962)
                     /gene="glpC"
                     /locus_tag="OHN27_003018"
     CDS             complement(8772..9962)
                     /gene="glpC"
                     /locus_tag="OHN27_003018"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCQ2287413.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWH
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9959..11218)
                     /gene="glpB"
                     /locus_tag="OHN27_003019"
     CDS             complement(9959..11218)
                     /gene="glpB"
                     /locus_tag="OHN27_003019"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCQ2287414.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11208..12836)
                     /gene="glpA"
                     /locus_tag="OHN27_003020"
     CDS             complement(11208..12836)
                     /gene="glpA"
                     /locus_tag="OHN27_003020"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCQ2287415.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13109..14467
                     /gene="glpT"
                     /locus_tag="OHN27_003021"
     CDS             13109..14467
                     /gene="glpT"
                     /locus_tag="OHN27_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCQ2287416.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            14472..15548
                     /gene="glpQ"
                     /locus_tag="OHN27_003022"
     CDS             14472..15548
                     /gene="glpQ"
                     /locus_tag="OHN27_003022"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCQ2287417.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(15590..15832)
                     /locus_tag="OHN27_003023"
                     /pseudo
     CDS             complement(15590..15832)
                     /locus_tag="OHN27_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            16011..16661
                     /gene="inaA"
                     /locus_tag="OHN27_003024"
     CDS             16011..16661
                     /gene="inaA"
                     /locus_tag="OHN27_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCQ2287418.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(16715..16969)
                     /gene="yfaE"
                     /locus_tag="OHN27_003025"
     CDS             complement(16715..16969)
                     /gene="yfaE"
                     /locus_tag="OHN27_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCQ2287419.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16969..18099)
                     /gene="nrdB"
                     /locus_tag="OHN27_003026"
     CDS             complement(16969..18099)
                     /gene="nrdB"
                     /locus_tag="OHN27_003026"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCQ2287420.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(18333..20618)
                     /gene="nrdA"
                     /locus_tag="OHN27_003027"
     CDS             complement(18333..20618)
                     /gene="nrdA"
                     /locus_tag="OHN27_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCQ2287421.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4930 a         5151 c         5667 g         5333 t
ORIGIN      
        1 atgtcgctca atttcacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
       61 ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt actggcgctc
      121 gctgcaattg ccggactgtg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      181 gacaataaaa cattggccag actggcacag cagcacccgg ttgtcgtttt gtttcgccat
      241 gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtt
      301 aaaggtaccc aggatgcccg tgaactgggc aacgctttta gcgctgacat ccctgatttc
      361 gatctttatt ccagtaatac cgtccggacc attcagtcgg cgacctggtt ttccgcgggt
      421 aaaaagctga cggtagataa acgacttctt cagtgcggta atgagattta tagtgcaatt
      481 aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca taatcattgc
      541 ctgacatata ttgctaaaaa taagcgtgat gcgacattta aacctgatta tctggatggt
      601 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taaccactaa
      661 attttcaatc tgacagccag tgatggctgt catcattgtt aaagaagacc tttcaaacgt
      721 aacgtttttc gggtggcaac atttaaatca taatgcacca gatcttcagg ttttacccac
      781 gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac gcagtcaaaa
      841 atcaggtaaa tcatataaat ctcttccttg cgaccatctg catacgtctt ggtgcgaata
      901 tcatcgctga aggtccacgg cgtgatttct gtcaaaagca gctgttctcc cagttcttcg
      961 cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccag actccacgcc gccacccgaa
     1021 agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag caaataagca
     1081 ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcataggggt attccttata
     1141 aataaaaaga aaccatgaga atacggtaac tcagaaatat gtgaaagtga cagtaatctc
     1201 ttttgcaatg ataactgcac ttattattat ttttgtgtgt atcggtaaag tgtgtttatt
     1261 ttttcggcgt ttaattattt aagaagtatt tagttacaat aagtgactag atcccttttt
     1321 tcgctcattg aatcctctgt gccaggcagt acaatcccgc agctttggga attttgatta
     1381 attgcttgtg ggaaagggga taatgaaaaa aattgcgcta gcaggtctgg ccggaatgct
     1441 gttggtttct gcatcggtca atgcaatgag catcagcggc caggcgggta aagaatacac
     1501 caatattggt gtcggttttg ggactgaatc gacgggccta gcgttaagcg gtaactggac
     1561 acataacgac gacgacggtg acgtcgcggg cgtggggctg gggttgaatc tgcctctcgg
     1621 gccgttaatg gcgaccgttg gcggaaaagg cgtttacacc aacccgaatt acggcgatga
     1681 aggttatgcc gcagcggtag gaggtggttt gcagtggaaa attggcaaca gcttccgttt
     1741 gtttggcgag tattactact ctccagattc gctctccagc ggtattcaaa gttatgagga
     1801 agcgaatgct ggcgcgcgtt acaccattat gcgtccagtc agtattgagg cgggttatcg
     1861 ctacctgaat ctgtcgggta aagacggtaa ccgcgacaac gccgtggctg acggcccgta
     1921 tgttggggtt aacgccagtt tctgatccct tccggcgcgg cattctgtcg cgccattcag
     1981 tttcttacta cgccttatct ccctttgcgt tatagtgatt cgataacaaa agcaggagat
     2041 aaccatgtta aaagtggaaa tgttatccac cggggatgaa gtgttacacg ggcaaatcgt
     2101 tgacactaac gctgcctggc tggccgattt tttctttcat caggggctgc cattatctcg
     2161 ccgcaatacg gtgggggata accttgatga cttagtcacc attcttcgcg aacgtagtca
     2221 gcacgccgat gtgctgatcg ttaacggcgg gctgggaccg accagcgatg atttaagcgc
     2281 actcgccgct gcgacagcaa aaggtgaagg cctggtgctg catgaagcct ggctcaaaga
     2341 gatggaacgc tatttccacg aacgtggacg agtaatggca ccgagcaacc gtaaacaagc
     2401 ggagctgcct gccagtgctg aatttatcaa taacccggta ggcaccgcct gtggttttgc
     2461 cgtgcagctt aatcgttgcc tgatgttctt tactcccggc gtaccgtcag aatttaaggt
     2521 gatggtcgag cacgaaatcc tgccgcgcct gcgcgagcgt ttttctttac cacagccgcc
     2581 ggtttgtctg cgtttaacta cctttggtcg ttcggaaagc gatctggcac aaagcctgga
     2641 cactctacaa ctgccgccgg gcgtaacaat gggctatcgc tcctcaatgc cgatcatcga
     2701 actgaaactc accggaccgg caagcgagca acaggcgatg gaaaaactgt ggctggacgt
     2761 taaacgtgtt gccggacaga gcgtgatttt cgaaggcacc gaaggactgc ccgcgcagat
     2821 cagtcgcgaa ttgcaaaacc gccagttcag cctgacgttg agcgagcaat tcaccggtgg
     2881 tttattggct ttgcaacttt ctcgcgcagg tgctccattg ctggcgtgtg aagtggttcc
     2941 ttcacaggaa gaaaccctgg cgcaaactgc gcactggatt acagaacggc gggccaacca
     3001 ttttgccggg ctggcactgg ctgtttcggg tttcgagaac gagcatctca actttgcgct
     3061 agccacgcca gacggcactt tcgctctgcg tgtgcgtttc agcactacac gctacagcct
     3121 ggctatccgt caggaagtgt gcgcaatgat ggcactgaat atgctgcgcc gttggttaaa
     3181 cggccaggac atcgccagtg agcatggctg gattgaggtt gttgagtcca tgaccttatc
     3241 tgtctgattc cgccactctg tgccggttta ctgcaccggc acattccccc tccggcaaaa
     3301 tttgtccaac ctgttgtact caataagaaa aaatccgtgc gctgattcac aaatttgtgc
     3361 gtaatttctc cagtccgctg gacgaggagc gggatttgat gcgcttcaca attttactcc
     3421 ccctgagcaa cgagactgat tccagtatct aagactggag tccagtatgt tggaatcaag
     3481 caaagttccc gccctgacac gggctattga tattctcaat ttgattgccc gcattggccc
     3541 ctgtagtgct gcgacgatca ttgacacatt gggaatccct aaaagcacgg cctatttgct
     3601 gcttaatgaa ctcaggcgtc agcgttttct tagccttgat caccaggaaa atttttgttt
     3661 gtggaccagg ctggtggagc tttctggtca tgcgttaagc aagatggatc tccgggaact
     3721 ggctcgaccg cgtttgacgc aactgatgga taccaccggg ctgttatgtc acctggggat
     3781 tattgataac ggcagcgctt attacatttt aaaagtggag tcatcagcca ctatcagcgt
     3841 gcgttcccat gaaggaaaaa gcctttcgct ttatcgctcc ggaataggta aatgcctgct
     3901 tgcctggcaa cctgcagcgg tacagcaaag tattatcgaa gggctggtct gggagcaggc
     3961 gacacccaca acgattactc atccacagca acttcacgaa gaactggcgc gtattcgccg
     4021 tcagggctgg agctatgaca acggcgaaga ttaccccgat gtccgttgtg tcgctgcgcc
     4081 ggtgtttaac gccaataacg agcttaccgc tgcgatctcg gtggtcggta cccgcttaca
     4141 aatcaatgaa gaataccgtg attatctggc gggcaaggcc attgcctgcg ccagggatat
     4201 ttcacgtctg ttgggatgga aaagtccatt tgacttacag gcctcataac aatggagaac
     4261 atcatgaccc taccaaaaat caaacaggtt cgcgcctggt ttactggcgg tgcgacagca
     4321 gaaaaaggtg ctggcggcgg tgattatcac gaccaggggg cgaatcactg gattgacgat
     4381 catattgcca ccccgatgag taaataccgc gattacgagc agtcacgcca gtcatttggc
     4441 attaacgttc ttggcacgtt gattgttgaa gtcgaagcag aaaacggcca gaccggattc
     4501 gctgtttcga cagccggtga aatgggctgt tttattgtcg aaaaacatct taaccgtttc
     4561 attgagggta aatgtgtcag tgatatcaaa ctgatccatg atcaaatgct caatgccacc
     4621 ctgtattact ccggctctgg tggcctggtg atgaacacga tttcctgtgt cgatctggct
     4681 ctgtgggatc tgttcggcaa agtggtcggg cttccggttt ataaactttt aggcggcgca
     4741 gttcgtgatg agattcagtt ctacgccaca ggcgcgcgtc cggatctggc aaaagagatg
     4801 ggctttatcg gtggcaaaat gccgacgcac tgggggccac atgatggcga tgcagggatc
     4861 cgcaaagatg ccgctatggt cacggatatg cgtgaaaaat gcggtgagga tttctggtta
     4921 atgctcgact gctggatgag tcaggacgtg aactatgcca ccaaactggc ccacgcttgc
     4981 gcgccctata acctgaaatg gatcgaagag tgcctgccgc cacagcagta tgaaggttat
     5041 cgcgaactga aacgcaacgc gccagtcgga atgatggtca ccagcggtga gcaccacggc
     5101 acactgcaat cttttcgtac gcttttagaa accggtatcg acattatgca gccggatgtt
     5161 ggctggtgcg gtggtttaac cacgctggtg gaaattgccg caatcgccaa atccaggggg
     5221 caactggtgg tgccgcacgg ttcgtctgtt tactctcacc atgcggtgat caccttcacc
     5281 aatacgccat tcagcgaatt cctgatgacc agcccggatt gttcaacgat gcgtccgcag
     5341 tttgacccga ttctgctcaa tgagccggtt ccggtgaatg gtcgtattca taaatcagtg
     5401 cttgataaac ccggttttgg cgtcgaactc aatcgtgact gcaatttgaa acgcccctac
     5461 agccactaat cactccgggc gttgctgtga caacggtaac gcccacatat tctaaggagc
     5521 tttttatgag caccgctttg cttgacgccg tggtgaagaa aaaccgtgtg cgtttaattc
     5581 cgtttatgtt ggcgctgtat gtgctggcgt ttctcgaccg ttcgaatatc ggttttgcca
     5641 aacagaccta ccagattgat accgggttga gtaatgaagc ttatgcgctg ggagcaggca
     5701 ttttctttgt ggtatatgcg tttctgggtg ttccggcgaa tcttttgatg cgcaaactgg
     5761 gggccagaac ctggattggt acgacaacac tgctgtgggg atttctttcg gcagccatgg
     5821 catgggccga tactgaagcg aaatttctga tagttcgcac tctgcttggt gctgcggagg
     5881 ccggattttt ccctggtatg atttatctca cctcgcaatg gtttctgcag cgtaatcgcg
     5941 ccagcattat ggggctgttc tatatgggcg caccgctggc gttaacactg ggatcaccgc
     6001 tttccggcgc gctgttggag atgcatggat ttatggggca tcccggctgg ttctggatgt
     6061 ttgtgattga aggattgttg gcagtcggtg ctggggtatt cacattcttt tggcttgatg
     6121 acacaccgga gcaggcacgt tttctgagta aacaagaaaa aacgttgctt atcaatcaac
     6181 tggcaagtga agaacaacag aaagtgactt ctcggctgag cgatgcgctg cgtaatggcc
     6241 gagtctggca actagcgatt atctacctga ccattcaggt ggcggtttac ggattaattt
     6301 tcttcctgcc gacccaggtt gcggcattgc tgggaacaaa agtgggcttt acagcgtcgg
     6361 tggtcaccgc cattccgtgg gttgcggcct tgtttgggac ctggcttatt ccgcgctatt
     6421 ccgataaaac cggcgaacgg cgtaatgtcg cagcgctgac attactggcg gcgggcattg
     6481 gtattggtct gtccgggctg ctttctccag taatggcgat cgtagcgctg tgtgttgcag
     6541 ctatcgggtt tattgccgtg cagccggtgt tctggacgat gccgacacaa ctgctttccg
     6601 gtacggcgct ggctgcgggg attggttttg taaacctgtt tggtgcagtg gcgggtttat
     6661 tgccccgatc ctgcgcgtga aagcagaaac gttatttgcc agcgatgcgg caggattact
     6721 gacactggca gcggtggcgg tcatcggttc gctgattatt ttcactctgc gtgtaaatcg
     6781 tactgttgcg cagaccgacg tggcacatca ttacataggt taaggaacac gatgaacgca
     6841 ttattaacca atccctttaa agaacgttta cgcaagggcg aagtgcaaat tggtctgtgg
     6901 ttaagttcga ctacttctta tatggcagaa attgccgcta cttccggtta tgactggttg
     6961 ctgattgacg gggagcacgc gccaaacacc attcaggatc tttatcacca gctacaggcg
     7021 gtagcgccct atgccagcca acccgtgatc cgtccggtgg aaggcagtaa atcgctgatt
     7081 aaacaagttc tggatattgg cgcgcaaact ctattgatcc cgatggtcga tactgccgat
     7141 caggctcgtc aggtggcgtc tgccacgcgc tatcctccct acggtgagcg tggtgttggg
     7201 gccagtgtgg cacgggctgc gcgctgagga cgcattgaga attacatggc gcaagttaac
     7261 gattcacttt gtctgttggt gcaggtggaa agtaaaacgg cactggataa cctggacgag
     7321 atcctcgacg tcgaagggat tgatggcgtg tttattggac cagcggatct ttctgcgtcg
     7381 ttgggctacc cggataacgc cgggcacccg gaagtgcagc gaattattga aaccagtatt
     7441 cggcggatcc gtgctgcggg taaagcggct ggttttctgg ctgtggctcc tgatatggcg
     7501 cagcaatgcc tggcgtgggg agcgaacttt gtcgctgttg gcgttgacac gatgctctac
     7561 agcgatgccc tggatcaacg actggcgatg tttaaatcag gcaaaaatgg gccacgcata
     7621 aaaggtagtt attgatatca aaggcccatg gggatcggct gtgggcctgt gttaattagt
     7681 ggttattcgc tgccagatcg gcttcgctta gctggatggc cgcgagcacc tggtcacgat
     7741 caatgccctg ttccagcatg cgcaaagcaa ttttaatggc ttgttcatgc ataccttcct
     7801 gccagccaat ttgatgccct tcctgccgta gccgttcagc aatagtcatt aatctctcct
     7861 tttgtagtgg tgaacgttcg gcaatctcct caataaaacg ggtgaaacgg gaggtatcgc
     7921 cgcattgcag cagataatta aacagtgttt gtagctggct gtcattagtg aagcctctaa
     7981 ccaaaagcgt ggtgatcctg tcgaccatgc cgattaaatc gcggtcgcga atatgctttt
     8041 gaatcagttc cagcagagct atacgccgat gttgcatgat ctcatcgtca ggtacgatgg
     8101 tgatatccac caacggaaaa gcttcggtgt acaactgccg ggcaagttgc ggctcgtcaa
     8161 actcatccag ccagttaagt gagtacgggt agggcgatgt ttcgccatga taaaacagca
     8221 atggcaccac cagcggaact ctgtcatagc ctttatccag gtgacgctgc atggcggcag
     8281 tggcatagcg cattagccga aaagccatat tcttttctgc agagctttga tgttcaatca
     8341 cgcagtagat atagccgtca ccgtcgctgg tttccacgga ccacaaaaca tccgagtagt
     8401 aagcgcgtaa acttttttca ataaaactag tgggctccag gcgtaaggtt tgcaggttgc
     8461 aaagcttgcg cagtggttct ggtaaatgta tttcgagaaa atcccgtgcg gtttcgggtg
     8521 tgaacataaa ggttttaaac accgcatcat gcggggagga tgttgttgat tcggtcatgg
     8581 ttgtccgtca cctgatattt atgatgacgc gacattacgt ctatcccggg cctttgacat
     8641 ctgtcagttt cagcctttgc gagacatttt cagaggaatt ttcaaagcgt tgcaacgcat
     8701 acatggtgcg tggaatcgcg ctcgcaaaaa tgcccgataa gcaatgtgct taccgggcat
     8761 caaaaggtcg gttaagccag cgcctgggcc agtagcgtaa tcggatgttc gcagcgaagg
     8821 ctcgtggaca tctcaatctg ccatttgcag gtttcgcagt cggtgatgac cagatccgcg
     8881 ccgctctctt ctatctggcg gaacagtggc gcaccgatgg cttgtgaggt ggggtagttc
     8941 tcttttttga aaccgtaagt acccgcaata ccgcagcact gggaatccag caccgttaac
     9001 tcaagccccg ggattttacg caacagctcc agggtgtaga gcgtccagcc cattttttcc
     9061 atatggcacg gagtgtgata aaccactttc agcggcagcg gtttcagcgg taacgttttg
     9121 ccttcgtcca gcttgtgcca cagccagcgg gttgccagtt cgatatgatc gcgcaagcct
     9181 ttgttgtcga cattcagcac ttccgggtat tcgtcgcgca gggcaaatgt acaggttgag
     9241 gaggtggcaa tcaccggaat gccttttact cccacagctt cgcggatcga ctctacattc
     9301 gtaattgcct gtttgcgtgc tttatcggta aagccgttgg cgattagcgg tacgccgcag
     9361 catttttctt tgctgagcag ttgtacaccg gtacccattg cgttgagcac tttaattaaa
     9421 tctttaccta actgcggatg gttgtagtta acgaagcagc cgtgaaagaa agcgacctgg
     9481 tctttatatt gtgcttgctg agccgctatg ctgcgatacc agcgacggaa cgtgccgaag
     9541 gagtatttcg gtagcgtgcg gcgatgatcg atttttaacg ccgcatcaag cagctgccgc
     9601 accggtttca gcgaggtagc ggtgttgacg attggtgcga acggcgtgga aacgctaccc
     9661 atcaggtcgg tatgactcaa cacaaaatta cgcagcgacg ggcgcgtggt gtcatatttt
     9721 gcccgcgcgc gctggataat atcgccaatc ttcacatcgg acgggcaggc gacttcacaa
     9781 cgtttgcagt tgatgcaata tttcagcgcc tcgtcataca gtgcgccatc tttcaaacgc
     9841 agacgctcgc catccggccc ggcttgtttt ggccctggat aaccgggatt cacccggctc
     9901 accgggcagg cggtggtgca gacggtgcac ttaatgcagt tttcgaagct ggtgtcgttc
     9961 attgttggcc tcctgcgcgt tgggcaatct gttgagcggc atgtaaagca ctgacggcac
    10021 aaacaccgcc gccgcatccc tgggcgatgg gatcaaatcc gcccagcacc gaaccgatgg
    10081 caaacaggtt ctcaatggtt tgccctgcct gcgacgggcg tagcgtctca tcagtggtta
    10141 caccgaactg ctgccacggt tgcggcgcaa aaaaatcgcc cttataccat tcaccccgcg
    10201 tggcggtttg tagcacatca aggccgagaa tcggctcgcg aatgccgtta cgttccgcta
    10261 ccagtccgcc actaaagaaa ctgccgctgg cgagaaccgc gaaacgtgga cgtagcggaa
    10321 tatcggcgtg attgcgggtc cagatttcat tcactacgcc atttttacag gtcacttttt
    10381 tcacttcatc gcccggcatc cacacgccac cctggcgcac aaactggcgc tgtaactggt
    10441 tttgcagacg aatgcccagc acggaaggcg gcagcgttgg caaaagcatc agtgaacaag
    10501 gtagtttttc attcaaccaa cgccacagtt tgtcatcggc caggccgaag caggcgggca
    10561 tcaggatcat ttcgcaggta ttggcgacag gaataagcgc atcaagtaac agcggccagt
    10621 tttcttcatt atcaaggaaa cgggcgatat tcaccgcgcg aaattcggtg gcgttattgc
    10681 gcaggacatc cagttccggc agctctattt ctgcggtttc aaccgaaaga tccagttcgc
    10741 gcaacgaagc cgccgcaaga tgcgcctgga aatccatcag cccgcttatc cccactacac
    10801 atatcttctt cgcgggtagc ggccagacgg gaacttctgg cgaacttaac caggttgcac
    10861 gcaaagtgcc gagcggggta acgcgctgat gggccagttc cacgctgccc tgcaattgcg
    10921 caccgctttc agcgatcaat gcctgcgcct ggcaagcgag atcgagcacg cgttgtggtt
    10981 cgagaagact gtaaggatgg gctggtgcct gctgacgcaa agattccagt ccactgtgaa
    11041 tgtctgtcac cggttgacca tcaggcagat ggctcagcaa atccagcgaa ccggatgaga
    11101 aatgcagcgc gctttgacca cgcgtgacaa tggcacagcg caggccgtgt ttttgcagtt
    11161 gcaggccaca gagtaatccg gcgaggccgc cgcccataat gacagtatca aagcgcatct
    11221 ttctgctcct tctccagacc acacagtccc tgataaaccc agcgggtaaa ttcgctttcg
    11281 cgcagtgcgt ctccccaggc gatgggttgc acgcctttcc agcgttcgtt aaggaaggtg
    11341 gaaagttgct cgatagattg cgcggacgtc gtgacgttaa accgttgcag cagtccggca
    11401 gcgcggcagg cgcagagttc gccctggcag gtgcccatcc ccacacgggt acgacggcgt
    11461 aaatccagca ggctattaac gtttaaattt tctaccgcgt actgcacttc acccgcagtt
    11521 accgcttcgc actcacatac caggctacgg tgcagacggc cttcgctcag ccaggcaggc
    11581 gtgcgatcgc catgacgata aaccgcagaa ccgcgcagcg gggcaggcag ggagatgact
    11641 ttacgcaagg taacttcagc gggttcttgt gaaccaggca gtgccagatc ggcagtcgta
    11701 caggggcgcg tgttgcccag tttgcggcat accgcgtcgg tagcccattc agccatcagc
    11761 cgataggtca tcagtttgcc accggtgatg gtgataaatc cgtccagacc atcgcgttca
    11821 gcatggtcga gcagcacgat gccacggctg acgttacgtc cgctcgggtc gtcatcgctg
    11881 gcaaccagcg ggcgcacgcc agaataggcc cgcaaaatgc gcgttttcgc catcacgggg
    11941 gccagttttt ccccttcacg cagcagaata tcaacctctt ctgccgtcac tcgattatcg
    12001 tcaatctcgt tgtaatcaat acgtaaagag gtggtaccaa tcagcgaaat ggtatcgcca
    12061 ggcaccagaa tatcggcgtc ggaaggttta cggcagcggt tgatcacatg ctggttaatg
    12121 cggtgatcca tgatcagcag cgatcctttc gccgggaaca tgcgaatgcg cagatcggca
    12181 tattcggcaa tgtgttgccc ccagatccca gcggcattaa ccacgacagg tgcatgaagg
    12241 gcctgagttt cgccggtgag atggttacgt acacgaacac cgcacaccgt cgcgccttca
    12301 cgaatcagcc ccgtgacttc atgagcggta aggataacgg caccgtgttc tttggcatcc
    12361 agcatgtttg ctgcggtcag acgaaatgga tcaacggtgc catccggaac tttcaccgcg
    12421 ccaatcagtg ccgggttaac ggcaggttcg ataatgcgcg cttgctgcgg gtctatagct
    12481 tctgcgctga tccctgcttc ttcgcaggcg cgaataaaag tggcctggaa tgagaggtca
    12541 tcttccggca gggtgataaa caggccgttg gttggttcaa cgcagtgacg tgcaatgcgt
    12601 ttcaggatct ggttttcact aatgcattcg cgggccgatt ccgcatcggt taccgcatag
    12661 cgcgcaccgc tgtgcagcag gccgtggtta cgcccggtgg caccggttgc gatgtcgtgg
    12721 cgctcaacca aaatcacgcg cagcccgcgc agggcacagt cgcgggcaat cccggctccc
    12781 gttgcgccgc caccaatgat aatcacgtca cttgattgcg agtcgcgagt tttcattgtt
    12841 atccctctga agttcgtttt ttaccattta gccatagtaa aaacatgaat tgtttgattt
    12901 cgcgcatatt cgctcataat tcgaaagtga aacgtgattt catgcgtcat tctgaacatt
    12961 ttgtaaatct tatttaataa tgtgtgcggc aattcacatt taatttctga atgttttctt
    13021 aacatcgcgg caactaaaga aacggcaggt tctcccactg aatcagtctg ttaatcataa
    13081 ataagaccac gggccacgga ggctttcaat gttgagtatt tttaaaccag cgccacacaa
    13141 agcgcgctta cctgctgcgg agatcgatcc gacttatcgt cgattgcgct ggcaaatttt
    13201 cctggggata ttctttggct atgcggctta ctatttggtt cgtaagaact ttgcgcttgc
    13261 tatgccttat ctggtcgagc agggattctc tcgcggtgat ttaggtttcg cgctttcggg
    13321 gatctcgatc gcttatggat tttcgaaatt cataatgggt tcggtatcag atcgctcgaa
    13381 tccgcgcgtt ttcctccccg caggtttgat tctggcggcg gcagtgatgt tgtttatggg
    13441 ctttgtgcca tgggcgacgt cgagcattgc ggtgatgttt gtacttttgt tcctctgcgg
    13501 ttggttccag gggatggggt ggccgccgtg tggtcgtact atggtgcact ggtggtcgca
    13561 gaaagaacgt ggcggcattg tgtcagtgtg gaactgtgcg cacaacgttg gtggtggtat
    13621 tccgccgctg ctgttcctgc tggggatggc ctggttcaat gactggcacg cggcgctcta
    13681 tatgcccgct ttctgcgcca ttctggtggc attattcgcc tttgcgatga tgcgcgatac
    13741 cccgcaatcc tgtggcttgc cgccgatcga agagtacaaa aatgattatc cggacgacta
    13801 taacgaaaaa gcggaacagg agctgacggc gaagcaaatc ttcatgcagt acgtactgcc
    13861 gaacaaactg ctgtggtata tcgccatcgc caacgtgttc gtttatctgc tgcgttacgg
    13921 catcctcgac tggtcaccga cttatctgaa agaggttaag catttcgcgc tggataaatc
    13981 ctcctgggcc tacttccttt atgaatatgc aggtattccg ggcactctgc tgtgcggctg
    14041 gatgtcggat aaagtcttcc gtggcaaccg tggggcaacc ggcgttttct ttatgacact
    14101 ggtgaccatc gcgactatcg tttactggat gaacccggca ggtaacccaa ccgtcgatat
    14161 gatttgtatg attgttatcg gcttcctgat ctacggtcct gtgatgctga tcggtctgca
    14221 tgcgctggaa ctggcaccga aaaaagcggc aggtacggca gcgggcttta ccgggctgtt
    14281 tggttacctg ggcggttcgg tggcggcgag cgcgattgtt ggctacaccg tggacttctt
    14341 cggctgggat ggcggcttta tggtaatgat tggcggcagc attctggcgg ttatcttgtt
    14401 gattgttgtg atgattggcg aaaaacgtcg ccatgaacaa ttactgcaaa aacgcaacgg
    14461 aggctaatgg catgaaattg acgctgaaaa accttagcat ggcgatcatg atgagcacta
    14521 tagtcatggg aagcagtgca atggcggcgg acagcaacga aaaaatagtc atcgcccatc
    14581 gcggtgccag tggatatttg ccggagcata cgctgccagc aaaagcgatg gcgtatgcgc
    14641 agggagcgga ttatctggaa caggatttgg tgatgaccaa agacgaccat ctggttgttc
    14701 tgcatgacca ttacctcgat cgtgttactg atgttgccga tcgtttcccg gatcgggcgc
    14761 gcaaagacgg tcgttactac gcgatagatt tcacgctgga tgaaattaag tcgttgaaat
    14821 ttaccgaagg tttcgatatt gaaaacggta aaaaagtgca gacttatccg gggcgtttcc
    14881 caatgggtaa gtccgacttc cgggtgcaca cctttgaaga agagattgaa tttgttcagg
    14941 ggttaaatca ctctaccggg aaaaatatcg gtatttatcc agaaatcaaa gcgccgtggt
    15001 tccatcatca ggaagggaag gatattgcgg caaaaacgct ggaagtgctg aagaaatatg
    15061 gttacaccgg taaagacgat aaagtttatt tgcaatgttt tgatgctgat gagctgaagc
    15121 gtattaagaa tgagctggaa cccaaaatgg gcatggagct caatctggta cagctgattg
    15181 cctataccga ctggaatgaa acgcagcaga aacagccgga tggaagctgg gttaattaca
    15241 actacgactg gatgtttaag ccgggtgcca tgaaacaggt ggcggaatat gcagatggta
    15301 ttggtccgga ttaccatatg ttgattgagg agacatcgca gccgggtaat atcaaactca
    15361 ctggcatggt gcaagatgct cagcagaata aactggtagt gcatccttat accgtgcggt
    15421 cagataaact gcctgaatac actcctgatg tgaatcagtt atatgatgct ctgtataaca
    15481 aagcgggtgt aaatgggctg tttactgatt tccctgataa ggcagtaaaa tttcttaata
    15541 aagagtaatg ttactgcagg atggcgttca tgcgccatcc tgtctcatat caaatttttc
    15601 caatcgccac taattgttcc atgcacattt gtagtaaaaa cagtggactg ccttctaccg
    15661 gcttttcttt agccagcaac gaaatctgtc gatagaaagt tggttcgaaa ggaacggaac
    15721 acaattcacc tgcaatgctt ttcagcgtta actctggttg taaggcgatg cctaatccct
    15781 gtcgaataaa attcatcgct gtatccgcct gattaaattc acattttatc tgctgggcgc
    15841 actattagtg gtagtggctg gtggaatcta tgcgctgcaa atgaagggta aaagcggtgt
    15901 cacgctagta gtggcaaaag aaattcatta atacgacacg tttcattaag attttcctca
    15961 ggtaaccacc tatagtcatt gtcgttaata aaaatgacag gcgatagggt atggcagttt
    16021 cagcaaagta cgacgaattt aatcactggt gggcaacaga gggcgactgg gttgaagagc
    16081 ctaactatcg ccgtaatggt atgagcgggg tgcaatgcgt cgagcgcaac ggcaaaaagc
    16141 tgtatgtaaa gcgcatgacg catcatctgt ttcattccgt acgttatccg ttcggccgac
    16201 caacgattgt ccgtgaggtt gcggtaatta aagaactgga acgggccggt gtcatcgtgc
    16261 cgaagatcgt ttttggtgaa gcggtgaaaa ttgagggtga atggcgagcg ttgctggtga
    16321 ctgaagatat ggcggggttc atcagcattg ctgactggta tgcccagcat gcagtatcgc
    16381 cttattctga cgaagtacgg caagccatgt tgaaagcagt ggcgctggcg tttaagaaaa
    16441 tgcatagcat taatcgtcag catggctgtt gttatgttcg ccatatctat gtgaaaacag
    16501 aaggtaatgc agaagctggt tttcttgacc tggagaaaag tcgtcgccgc ttgcgtcgcg
    16561 ataaagcgat taatcatgac ttccgccagc tggaaaaata tctggagcca atccccaaag
    16621 cggactggga gcaggtgaaa gcgtattact acgccatgta atcgttgtag gccagataag
    16681 acgcataagc gttgtatctg gcactacaac caattcacat ctcgatttca atatcgcctt
    16741 ttgcccggca acaacagggc aaaatttccc ccggctgaat aaaggctaac ggttcggcaa
    16801 tccagtcaac ctgacctgca accagacggg tgcgacagga gccgcagtaa ccttcgcgac
    16861 actgatactc aaccgccaca ttgtgggatt ccagcgccgc cagaagggaa gggtgttcat
    16921 cctggcacag cagttgtgtg ccagtgatgc gcagggtaac gcgggccatc agagttggaa
    16981 gttactcaaa tcgtcggtgt ccacttccgc gtcaatctgc ccgaccagat aagaactgac
    17041 ttccacttcc tgcggagcaa cctgcacgtt atcagacacc agccaggtgt tgatccatgg
    17101 gatcgggttg gagcgcgtct ggaacggcag atccaaaccg actgcctgca tacggatatt
    17161 ggtgatgtat tcaacgtact ggcagagaat gtctttattc agaccaatca tcgaaccgtc
    17221 gcggaacaga taatccgccc agtctttctc ctgttgagct gcctgaacaa acaggtcata
    17281 gcactcctgc ttacactctt cggcaatttc cgccatctca ggatcgtccg cgccgctgcg
    17341 cagcagattc agcatatgct gggtgccggt caggtgcagg gcttcgtcgc gggcaatcag
    17401 gcgaataatt ttggcgttgc cttccatcaa ttcgcgttct gcaaatgcga aggaacaagc
    17461 aaagctgacg tagaaacgaa tcgcttccag cgcgttaacg ctcatcaggc agagatacag
    17521 ttttttcttc agctcgcgca ggctaacggt cacagtttta ccgttaacgg tgtgggtacc
    17581 ttcgcccagc agatgccagt agctggtcat ttcgatcagc tcatcgtaat agctggagat
    17641 cccttccgca cgtttctgga tctgctcgtt ggtgacgata tcgtcaaaca caacagacgg
    17701 atcgttaacg atattacgaa tgatatgagt ataggaacgg gaatgaatcg tttctgaaaa
    17761 cgcccaggtt tcgacccagg tctccagttc cgggatcgag atcagcggca gcagtgccac
    17821 gttcgggcta cggccctgaa tggaatccag cagtgtctga tatttcaggt tgctgataaa
    17881 gatgtgtttt tcgtgttcag gcagtgcctg gtaatcgata cggtcgcgag aaacgtcaac
    17941 ttcttccgga cgccagaaga aagagagctg cttttcgatc agcttttcga agatgtcata
    18001 tttttgctga tcgtagcgag ccacgttgac cggctgacca aagaacatcg gttctttgag
    18061 ctgatcattt ttcgtctgtg aaaaggtggt atatgccatg agtgtgtcct gttgggagtt
    18121 taatgccgga taaggcattt tacgccgcat cctgtgcctg atgcgacgct ggcgcgtctt
    18181 atcaggccta caaatccgag ccgtaggccg gataaggcgt tcacgctgca tccggcaccc
    18241 ggagcctgat gcgacgctgg cgcgtcttat caggcctaca aaccgagccg taggccggat
    18301 aaggcgttta cgccgcatcc ggcatctcaa tatcagatct tacatgcgcc gctttcgcag
    18361 ccatcgtcct ggattgacgg caccagatcg tcctgcgcgt cttcagcgcc gtcacgggtg
    18421 ttctgataat acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc tttcagcaac
    18481 tgctgcatcg gcacttttcc tgacgggaaa cgtgacggat cgtagttggt gttagcagag
    18541 atcgactgat cgataaattt ctgcatgata cctaccagtt gcaggtagcc atcgttaccc
    18601 ggcatttccc acagcagctc ataggcgtcg tgcaggtgct cgtagtccgg caccacctgg
    18661 cgcaaaatac cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg
    18721 ttagtggcgt tagagatctg cgaagaggtc tcggacggca tcagggcaga aagcgtggag
    18781 ttacgcaggc cgtgcgtttt gattgattca cgcaatgctt cccagtcgta atgcagcggc
    18841 tcattagcga tggcatccag atctttctta taggtatcga tcggcaggat ccctttcgcg
    18901 taagtggttt cgttaaacca cgggcacgcg ccttgctctt tagccagctc attagacgct
    18961 ttcagcaggt aatactgaat cgcttcgaag gttttatgcg tcaggttgtt ggcgctgccg
    19021 tcggagtagc gtttaccgtg cttcgccaga tagtaagcga agttaatcac accaataccc
    19081 agcgtacgac gccccatcgc tccacgtttg gcggccggga tcgggtaatc ctgataatcc
    19141 agcagcgcgt caagtgcacg aaccgccaga attgccagct cttccagttc atccaggcta
    19201 ttaattgcgc ccaggttgaa agcagacagc gtacacagcg cgatttcacc gttctcgtcg
    19261 ttgacgtcgt tcagcggttt ggtcggcagg gctatctcca ggcacaggtt agactgacgc
    19321 actggcgcga tggccggatc aaacgggcta tgggtattgc agtggtcaac gttctgaata
    19381 tagatacgac cggtagacgc acgttcctgc atcatcagcg agaacagctc aacggctttc
    19441 acacgctgct tgcggatgct gtcgtctttc tcatatttgg tatacaggcg ttcaaactct
    19501 tcctgatcgg cgaagaatgc gtcgtacagc cccggtacgt cggacgggct gaacagggtg
    19561 atatcttcac ctttcagcag gcgggtatac atcagtttgt tgatctgtac cccgtagtcc
    19621 atatggcgca cgcggttgcc ttccacacca cggttgtttt tcaacaccag cagactttcc
    19681 acttccagat gccacatcgg gtagaacagc gttgccgcac cgccgcgcac gccgccctga
    19741 gagcaggatt tcaccgctgt ctggaaatgt ttgtagaacg gaatacagcc ggtatggaac
    19801 gcttcaccac cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt gatgccgatc
    19861 ccggcacgct gggaaacgta tttcacaatc gcgctggagg tagcgttgat ggaatccaga
    19921 ctgtcaccgc actcgatcag tacgcaggag ctgaactgac gagtcggggt acgcacgccg
    19981 gacatgattg gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc
    20041 acatattgca ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc aaccagaata
    20101 taaaggaact gggcgctttc gtagatttcg ccggtcacgc ggttctgtac cagatatttg
    20161 ccttccagct gcttaacggc agcataagag aaggtcatat cacggtcgtg atcgataaac
    20221 gtgtccatct gcttgaactc ttcttccgtg tagtcttcca gcagatgatt atcgtatttg
    20281 cccatctcga ccattttcac cacgtggtcg tacagcgcag gcggctcaaa ctggccgtag
    20341 gcttttttac gcaggtggaa gatcgccagg cgcgcggcga gatactgata atccggcgca
    20401 tcacgggaga tcaggtctgc ggcagccttg ataatggttt cgtggatgtc agaggtcttg
    20461 ataccgtcat aaaactgaat gtgggagcgc agctcgacct gggaaatcga aacgttatgc
    20521 agtccttctg ccgcccaatc cagaacgcga tggattttgt cgagattgat gcgctctgtg
    20581 ctaccgtcgc gctttgtcac cagcagattc tgattcatgt atgtcgtacc tgtttttgga
    20641 attctttccg caatagtttc atgcagacta cctgtagtac gggaagctgc ttagaacact
    20701 atatataggg ggtatgtttg ggattcactg caagatagtg tgaaaatggc cctcttgcaa
    20761 gtgcataact ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag
    20821 acaaggtgtc cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg
    20881 atttcttgaa cggcagaata ggttggataa attgatatag atggtttatt aatctttttg
    20941 ctaatgattg caaaaattga tctaagcaca agtcatcaca taatctgatg tatcagttgt
    21001 ggtcaatata taagcgaatg taagcgattg ttgatgtcga aatggttgtt ttctattcgg
    21061 ttattgttaa atgcgaagcc a
//
LOCUS       DAJOSY010000078        21049 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJOSY010000078 DAJOSY010000000
VERSION     DAJOSY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.078"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..158
                     /gene="pgaD"
                     /locus_tag="OHN27_003028"
     CDS             <1..158
                     /gene="pgaD"
                     /locus_tag="OHN27_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="in Yersinia this gene is involved in biofilm
                     formation and hemin adsorption; in Escherichia coli it is
                     involved in PGA biosynthesis and export; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HCQ2287422.1"
                     /translation="AYQYTPQEYAESLAIPDELYQQLQKSHRMSVHFTSQGQIKMVVS
                     EKALVRA"
     gene            complement(208..996)
                     /gene="phoH"
                     /locus_tag="OHN27_003029"
     CDS             complement(208..996)
                     /gene="phoH"
                     /locus_tag="OHN27_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCQ2287423.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1616..2887)
                     /gene="efeB"
                     /locus_tag="OHN27_003030"
     CDS             complement(1616..2887)
                     /gene="efeB"
                     /locus_tag="OHN27_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCQ2287424.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYFGSALLRV"
     gene            complement(2893..4020)
                     /gene="efeO"
                     /locus_tag="OHN27_003031"
     CDS             complement(2893..4020)
                     /gene="efeO"
                     /locus_tag="OHN27_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCQ2287425.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4078..4908)
                     /locus_tag="OHN27_003032"
     CDS             complement(4078..4908)
                     /locus_tag="OHN27_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCQ2287426.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(5450..6958)
                     /gene="putP"
                     /locus_tag="OHN27_003033"
     CDS             complement(5450..6958)
                     /gene="putP"
                     /locus_tag="OHN27_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCQ2287427.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFSSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(7117..7326)
                     /locus_tag="OHN27_003034"
     CDS             complement(7117..7326)
                     /locus_tag="OHN27_003034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287428.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            7381..11343
                     /gene="putA"
                     /locus_tag="OHN27_003035"
     CDS             7381..11343
                     /gene="putA"
                     /locus_tag="OHN27_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCQ2287429.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKVNIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(11382..12020)
                     /gene="rutR"
                     /locus_tag="OHN27_003036"
     CDS             complement(11382..12020)
                     /gene="rutR"
                     /locus_tag="OHN27_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCQ2287430.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFTQTVENVQRIIIEGIRPR"
     gene            12308..13399
                     /gene="rutA"
                     /locus_tag="OHN27_003037"
     CDS             12308..13399
                     /gene="rutA"
                     /locus_tag="OHN27_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCQ2287431.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMANPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            13399..14091
                     /gene="rutB"
                     /locus_tag="OHN27_003038"
     CDS             13399..14091
                     /gene="rutB"
                     /locus_tag="OHN27_003038"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCQ2287432.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFAHIA"
     gene            14103..14489
                     /gene="rutC"
                     /locus_tag="OHN27_003039"
     CDS             14103..14489
                     /gene="rutC"
                     /locus_tag="OHN27_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCQ2287433.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            14497..15297
                     /gene="rutD"
                     /locus_tag="OHN27_003040"
     CDS             14497..15297
                     /gene="rutD"
                     /locus_tag="OHN27_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCQ2287434.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            15307..15897
                     /locus_tag="OHN27_003041"
     CDS             15307..15897
                     /locus_tag="OHN27_003041"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCQ2287435.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFT
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            15908..16402
                     /gene="rutF"
                     /locus_tag="OHN27_003042"
     CDS             15908..16402
                     /gene="rutF"
                     /locus_tag="OHN27_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCQ2287436.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            16423..17751
                     /gene="rutG"
                     /locus_tag="OHN27_003043"
     CDS             16423..17751
                     /gene="rutG"
                     /locus_tag="OHN27_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCQ2287437.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDYTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(18008..18181)
                     /locus_tag="OHN27_003044"
     CDS             complement(18008..18181)
                     /locus_tag="OHN27_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCQ2287438.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            18554..19150
                     /gene="wrbA"
                     /locus_tag="OHN27_003045"
     CDS             18554..19150
                     /gene="wrbA"
                     /locus_tag="OHN27_003045"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCQ2287439.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            19171..19398
                     /locus_tag="OHN27_003046"
     CDS             19171..19398
                     /locus_tag="OHN27_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCQ2287440.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(19436..20677)
                     /gene="agp"
                     /locus_tag="OHN27_003047"
     CDS             complement(19436..20677)
                     /gene="agp"
                     /locus_tag="OHN27_003047"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCQ2287441.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTRAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
BASE COUNT         4953 a         5686 c         5452 g         4958 t
ORIGIN      
        1 cagcctacca atatacgccg caagaatatg cagagagctt agcaatacct gatgagctct
       61 atcagcaact acaaaaaagt cacaggatga gcgtacactt caccagccag gggcaaataa
      121 aaatggttgt ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt
      181 tgcccgggct ctgcaccgat aacacactta actgtaggca tgcagcgtac gttggcaaag
      241 tgccgaacgt acgcagtcct ctttaccgaa ccggacgatc ccaaccattt catcttcttc
      301 gaaacgttcc agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt
      361 gatatcaccg ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat
      421 ttgcgcggca gtcacattct gcgcctcgtc aagaatgacg actgcatttt caaaggtacg
      481 tccacgcata taggcgaacg gcgcaatttc caccttccct atttccggtc gcaggcagta
      541 ctgcataaag gaagccccta agcgccggac cagcacgtcg tagaccgggc gaaaataggg
      601 agcaaacttt tctgcgatat ctccaggtaa gaagccaaga tcttcatcgg cttgcagaac
      661 tggacgggtg acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc
      721 tgcgctgatc caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact
      781 ctcaatagcc ttcagatagt gcaattgcgc ttcatttcgc gcgaggatgg gcgaagtatc
      841 gcgactgtcg cgggccatac caatggcttc tacgccgccc atctgcacaa gcgaggtgac
      901 cgattcttct tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc
      961 gcgacgagct ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta
     1021 ttcatcacac gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc
     1081 ttgtaagcca tagtttgctt ttggataaaa tgccgaaaaa cggctacgcg caccgtttac
     1141 ggcgtcggta acacatgaaa agaaaggatg aggttgaaaa tgcaaagtga cgagatgact
     1201 accggaggag aaaactccgc gagtggtggc gcgttgatta tctaaaacat gtccagtaca
     1261 ggacgttacc atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc
     1321 tacttaataa gtacaacaga tctcgcattt attgcaacaa tatatttact tatatttaac
     1381 tataaaacac catttcagtg acattagttt ctactggaaa gatgacagag tgatgacagt
     1441 gatgaaaaaa gctgtgtgct ttcagcagga tttgcatcag gcatcagtgc acgatcgccg
     1501 gatgcggcgt gaacgcctaa ccagcctacc aaacccgcac tgattgtagg catgataaga
     1561 cgcgcaagcg tcgcatcagg catctgcgca caaatgcctt atccgcctaa aaacattaaa
     1621 cccgcaataa cgcgcttccg aaataatcgt tcgcgtcctt cacccccggc agcgcaaaaa
     1681 aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg agcctttttt
     1741 gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc aaccccatat
     1801 ccagttgccc ggagttggtg acgcccagtg aataactgta gccacgacgc agcatcaggc
     1861 tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc agcgcgatca
     1921 ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc attcccagcg
     1981 gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc ggcgttctgt
     2041 cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca ccgattgtcc
     2101 acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac ttatcattct
     2161 ggctatcggg attggcagtg ccgtctttga aacccagcaa attaatcggc gtctctttgc
     2221 ctttactacg cgccgcgtga tcggaaataa acccttcccg cttccagcgc acactgagca
     2281 aatccggcgt gtgtttgatg atatcgcgca gcgcatggat aaccgtgtcc tgggtgttgg
     2341 cgcaaatctg tagcaacaca tcaccatgac ataacgccgc atccagcgag tcgttgggga
     2401 aacgcgtcat cttctgcagc ttttttggca tctgtggcgc aaggccaaag cgctcatcaa
     2461 acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg tagccgccaa
     2521 gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgct gctccgccct
     2581 gagtcagaaa agcaaaacgc tgagtcaaca agcgaaacaa ccgctcaaga tcggctttat
     2641 cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc gtcaggatcc
     2701 ctgcctgatg ctcaccataa aacggctgtt tctcattgcg agcatccggt gaaagcgtac
     2761 ccggcgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc agcgccagtg
     2821 cacctatcac tttcagtaaa cgtcggcgtg acggttcatt cacgccgttt tcatctttat
     2881 actgcataac gcttaatcca gtcccagcac accgcgaagt tgcgccagat cttccgccag
     2941 cgcagtaatc ggtcctttca gtgcattccg gtcggcatcg gtcaatttgt cgtaggtttc
     3001 aaaaccgtct ttagtacggt atttcgccag aatggtatcg acctttttaa agttggcatc
     3061 gacttttgcc agcagttccg ggttggcttt ttgcagttgt ggacgcagca aatcgacaat
     3121 tttctgcgag ccttcaacgt tagcctggaa atcccacaga tcggtgtggc tgtagcgatc
     3181 ttcttcaccg ctaattttgc tggctgccac ttcctcaatc agtccggctg cgccgccgac
     3241 cacttttgaa ggtgggaaag ccagttcact gatgcgtttt tgcaaatcga ccacatcggt
     3301 ataaagctgc tcagcgtact gatccatccc tttggtggtg ttgtcgccaa acaatgcttt
     3361 ttccagacgg tggaaaccag tgaattttgg gtcggcggct ttttgctcgt aatcatcttc
     3421 acgggcgtca atgctgccat ccagatcgga gaacagttca gcaatcggtt caatacgctc
     3481 atagtgctgg cgcgtcggtg catacagtgc tttcgctttt tcgatatcgc ctgctttaat
     3541 cgcgtcggta aaggctttgg tgtcggtcac cagctgcgtg gtttccgcca tgacatacgc
     3601 tttatatgca gtaattgcac caccaagact taacagcgca tcactttgcg ccgcatccgc
     3661 cgttgcctca cctttgacga tcaacttccc tttcgggtta gtcagcagac cgcaggtcat
     3721 atcgtattcg ccaggctgta aattcgccgt cattttctgg ctaaagccag gggcgatatt
     3781 ttcccgctct tccaccacca tcacgccttt gaggatctcc cactccagcg ccttctggct
     3841 gtggttctga ataatgaact gtgttttccc ggcgttaacc gtaatggtca tcggttcgca
     3901 ctgcttatcc gtcacggtca ctttgacctg cggcacatca gcggcgttag ccataaaagc
     3961 agaagaaaac agcgcagcca cgctcaactg caatgcgtta cggcggaagt taatggtcat
     4021 gacgagacta tccctttaaa gtatgttgta actaagcaag agtttgcgtc gtatttgtta
     4081 cgcggagcga gacgctgtcg cccctgcgcg tggtggcaga gcaaatgcca ccagcgccgg
     4141 gatgagataa ataaaccaga cggcgacttc gctgacgctc ggcgcttcct gatagccaaa
     4201 aatcccttcc atcagcgtgc caaacagcga gtgagttgag agcaccgcac tcatatcgaa
     4261 ggcgatttcc tgaaagtggt tccacaatcc ggcttcatga aatgcgcgaa tggcaccagc
     4321 tgccagccct gcggcgacga agagaataaa caggctggtc catttaaaaa atgcaccaag
     4381 attgaggcga ataccgcccc agtagagcag gaagcctagc accacggcag tagcaagacc
     4441 gagcattgca cccagcggcg gccagatccc gacatcttgt tgaaatgccg ccagcaggaa
     4501 aaagaccgac tccagccctt cccttgcaac ggcaaaaaag accatcatca ccagcgccca
     4561 gccatgatga tttccacgct gcaatgcgct atcgactgcc tgttccagtt gcactttgac
     4621 gttgcgcgac actttgcgca tccagaaaac catccaggta aggatcacca cggcgatcac
     4681 cgccacgata ccttcaaaca gttcctgttc tttttgcgga aattcgccgg tggtttcgtt
     4741 aatgaagata cccaagccca ggcacaacgc agcggcaagc aacacgccaa tccacatcac
     4801 accaatccat cggcctcgct gggtacgctt aagatagctg gcaatcaaac tgacaatcag
     4861 cgcggcttca agtccttcgc gcaacataat gagaaacgga acaaacatgc cacccaccct
     4921 taaacgtttt tctgagtcaa tctatgcaaa gaaaagtaaa tcgcagtgat agcgattatc
     4981 attacggtaa agaaaaatac aagcggaatg tttataaaat gatgttgaaa tgtaaacaaa
     5041 gagaggtaat gccaggggat ataaacgatt attccaggta caaagatggg attgtttaca
     5101 acgagccatg tgttcttgcc ggatgccgcg taaacgccgt atccagccga caaaactgtc
     5161 cagactcaat gcatcagagc ttccatgtaa aactgataag cgggtgtatc aagcaatttc
     5221 gtccatccgc gattatcaaa tctaactttt tgcccggaac ataataacag ctcgggtgtt
     5281 ggtggcgtca aaatcgctga agcgttcccc gaaggccggg acagcagggg gcggcggcga
     5341 ttggccgccc cctgcgcgct ccctgcacca ttcaatttat atggcgaggc acattaaggg
     5401 agcgcaaccc gctgctgaag ccaaacaaaa ccgccgcagg ctaagtccct tagctttcct
     5461 gcaaccgtga cggcggagcc gaatgatagt gcgcatcggc ctcggcaaag cgtttttgca
     5521 tcgccgctga cggcgcttta cccagcaaac taaacactac aatcccaata ctgctgaaga
     5581 taaagcccgg aataatttcg tacagtccca gccagccgaa ctgtttccag acgataaccg
     5641 tcagcgcccc gatgatcatc cccgccagcg caccgttgcg cgtcatgcgt gaccacatca
     5701 ccgagaacag caccaccgga ccaaacgccg cgccaaagcc tgcccacgca tagctcacta
     5761 gccccagcac acggttttcc gggtttgccg ccagcgcaat ggccaccagc gccaccacca
     5821 gcaccatcac acgacctacc cacaccagct ctttctggct ggcatgttta cgcagaaacg
     5881 ctttgtataa atcttcggta atcgcactgg agcacaccag cagctggcaa cttaaggttg
     5941 acattaccgc cgccagaatt gccgacagca gaatcccggc aatccacggg ttaaacagaa
     6001 tttgcgccag ttcgataaac acgcgctcag cattctggtt taccgcacct gctaccgccg
     6061 ggtgctcatt aaagtaagca ataccaaaga agccgacagc taccgcccct gccaggcaga
     6121 ggatcatcca ggtcatactg atacgacgcg catggacaat gctgtggtga gagtctgccg
     6181 ccataaaacg cgccaggatg tgcggttgcc cgaagtaacc cagcccccaa cccatcagtg
     6241 agataatggc gacaaagttc agccctttga gcatatcaac gttttcgatg ctcttttgtt
     6301 tgatcacttc cagcgagtca ccaaagccac cgacactgat aatgacgata accggcgtca
     6361 ggatcagggc aaaaatcatc aggctggcct gtacagtgtc agtccagctc accgcgagga
     6421 aaccgccaat aaaggtgtaa aggatcgtcg ccgcagctcc cgcccacaga gccgtttcgt
     6481 agctcatgcc aaaggtactt tcaaacagac gcgcgcctgc cacaatgccc gaagcgcaat
     6541 aaatggtgaa gaacagcaaa atcaccagcg cggagataat gcgcaaaatg cggcttttat
     6601 cttcaaagcg cccggtgaaa taatccggca atgttaaggc gttattgttg tattcggtat
     6661 gcacacgcaa ccgccccgcc accagcttcc agttgatcca cgcgcctaat gtcaggccaa
     6721 tggcgatcca actttcggaa atcccggaaa gaaaaaccgc gcccggcaac cccattaaca
     6781 gccagccgct catatccgac gcacccgccg ataatgccgt cacgaatggc ccaaggctac
     6841 gaccgcccag aatatagtcg tcaaagtttt tcgttgatcg ccaggcgata aacccaatca
     6901 atatcatgcc aaagatatag acacaaaatg tcaccaacat cggtgtgcta atagccatct
     6961 aaagtctcca aaaaattatt atcggcaatg tcgaaacttg ccgttatatc tgccaccgga
     7021 acggggtaac agagtttatg ttttaccagg gcgaccgtat cctgccggaa gcgctggtta
     7081 ttcacaatcg atttaacaca ccatttacat taaattttag tgctcagcga cactattttt
     7141 catcaggttg cactctctca cattttttgc ggttgcacct ttcaaaaatg ttaactgccg
     7201 cagagaaaaa gtctgagtta tttttttaat ccctgtcata tcgatttctt ttattaacat
     7261 ttcattcatt tttaagcttg ctacgcatgt cacatttaac atggttgcac aaagttgcaa
     7321 catcatggat atttcacgat aacgttaagt tgcacctttc agaacaacag gagtaatggc
     7381 atgggaacca ccaccatggg ggttaagctg gacgacgcga cgcgtgagcg tattaagtct
     7441 gccgcgacac gtatcgatcg cacgccacac tggttaatta agcaggcgat tttttcttat
     7501 ctcgaacaac tggaaaacag cgatactctg ccggagctac ctgcgctgct ttctggcgct
     7561 gccaatgaga gcgatgaagc accgactccg gcagaggaac cacaccaacc attcctcgac
     7621 tttgccgagc aaattttgcc ccagtcggtt tcccgcgccg cgatcacagc ggcctatcgc
     7681 cgcccggaaa ccgaagcggt ttctatgctg ctggaacaag cccgcctgcc gcagccagtt
     7741 gctgaacagg cgcacaaact ggcgtatcaa ctggccgata aactgcgtaa tcaaaaaaac
     7801 gccagtggtc gcgcaggtat ggttcagggg ttattgcagg agttttcgtt gtcatcgcag
     7861 gaaggcgtgg cgctgatgtg tctggcggaa gcgttgttgc gtattcccga caaagccacc
     7921 cgtgatgcgt taattcgcga caaaatcagc aacggtaact ggcaatcaca cattggtcgt
     7981 agcccgtctt tgtttgttaa tgccgccacc tgggggctgc tgtttactgg caaactggtt
     8041 tccacccata acgaagccag cctctcccgc tcgctgaacc gcattatcgg taaaagcggt
     8101 gaaccgctga tccgcaaagg tgtggatatg gcgatgcgcc tgatgggcga gcagttcgtc
     8161 actggcgaaa ccatcgcgga agcgttagcc aatgcccgca agctggaaga gaaaggtttc
     8221 cgttactctt acgatatgct tggcgaagct gcactgactg ccgcagatgc acaagcgtat
     8281 atggtttcct atcagcaggc gattcacgcc attggtaaag cgtccaatgg tcgtggcatc
     8341 tatgaaggtc cgggcatttc aatcaagctg tcggcgctgc atccgcgtta cagccgcgcc
     8401 cagtatgacc gggtaatgga agagctttac ccacgtctga aatcactcac cctgctggcg
     8461 cgtcagtacg atattggtat caacattgac gccgaagagg ccgatcgtct ggagatctcc
     8521 ctcgatctgc tggaaaaact ctgtttcgag cctgagctgg caggctggaa cggtatcggt
     8581 tttgttattc aggcttatca aaaacgctgc ccactggtga ttgattacct gattgatctc
     8641 gccacccgca gccgtcgccg tctgatgatt cgcctggtga aaggcgcgta ctgggatagt
     8701 gaaatcaagc gtgcgcagat ggacggcctt gaaggctatc cggtttatac ccgcaaggtg
     8761 tataccgacg tttcatatct cgcctgtgcg aaaaagctgc tggcggtgcc gaatctaatc
     8821 tacccgcagt tcgcaacgca caacgcccat acgctggcag cgatttatca actggctggt
     8881 caaaactact atccgggtca gtacgagttc cagtgcctgc atggtatggg cgagccactg
     8941 tatgagcagg tcaccgggaa agttgccgac ggcaaactta accgtccgtg tcgtatttat
     9001 gccccggttg gcacacatga aacgctgctg gcgtatctgg tgcgccgcct gctggaaaac
     9061 ggtgctaaca cctcgtttgt taaccgtatt gccgacacct ctttgccact ggatgaactg
     9121 gtcgccgatc cggtcactgc tgtagaaaaa ctggcgcaac aggaagggca aactggatta
     9181 ccgcatccga aaattcccct gccgcgcgat ctttacggtc acgggcgcga caactcggca
     9241 gggctggatc tcgctaacga acaccgtctg gcctcgctct cctctgccct gctcaatagt
     9301 gcactgcaaa aatggcaggc cttgccaatg ctggaacaac cggtagcggc aggtgagatg
     9361 tcgcccgtta ttaaccctgc ggaaccgaaa gatattgtgg gctttgtgcg tgaagccacg
     9421 ccgcgtgagg tagaacaggc gctggaaagt gcggttaata acgcgccaat ctggtttgcc
     9481 actcctccgg ttgaacgcgc ggcgattttg catcgcgctg ccgtgctaat ggaaagccag
     9541 atgcagcaac tgattggtat tctggtgcgt gaagccggaa aaaccttcag taacgccatt
     9601 gccgaagtgc gcgaagcggt cgattttctc cactactatg ccggacaggt gcgggatgat
     9661 ttcgctaacg aaacccaccg tccattaggg cctgtggtgt gtatcagtcc gtggaacttc
     9721 ccgctggcta ttttcaccgg gcagattgct gccgcactgg cggcaggtaa cagcgtgctg
     9781 gcaaaaccgg cagaacaaac gccgctgatt gccgcgcaag ggatcgccat tttgctggaa
     9841 gcgggtgtac cgccaggcgt ggtgcagttg ctgccaggtc agggggaaac cgtgggcgcg
     9901 caactgacgg gtgatgatcg cgtacgcggg gtgatgttta ccggttcaac cgaagtcgct
     9961 acgttactgc agcgcaatat cgccagccgc ctggacgctc agggtcgccc tattccgctc
    10021 atcgctgaaa ccggcggcat gaacgcgatg attgtcgatt cttcagcact gaccgaacaa
    10081 gtagtaatag atgtgctggc ctcggcgttc gacagtgcgg gtcagcgttg ttcggcgctg
    10141 cgcgtgctgt gcctgcaaga tgagattgcc gaccacacgt tgaaaatgct gcgcggcgca
    10201 atggccgaat gccggatggg taatccgggt cgcctgacca ccgatatcgg tccggtgatt
    10261 gatagcgaag cgaaagtcaa tattgagcgc catattcaga ccatgcgtag caaaggccgt
    10321 ccggtgttcc aggcggtgcg ggaaaacagt gaagatgctc gtgaatggca aagcggcacc
    10381 tttgtcgccc cgacgctgat cgaactggat gactttgccg aattgcagaa agaggtcttt
    10441 ggtccggtgc tgcatgtggt gcgttacaac cgtaaccagc tcccagagct gatcgagcag
    10501 attaacgctt ccggttatgg tctgacgctt ggcgttcata cgcgtattga tgaaaccatc
    10561 gcccaggtca ctggctcggc gcatgtcggt aacctgtacg ttaaccgtaa tatggtgggc
    10621 gcagtagtcg gggtgcaacc gttcggcggc gaagggttat ccggcaccgg gccaaaagca
    10681 ggtggcccgc tctatctcta ccgtctgctg gcgaatcgcc cggaaagtgc gctggcagta
    10741 acgctcgcgc gtcaggatgc agagtatccg gtcgatgcgc agttgaaagc cgcattgact
    10801 cagccgctaa atgcactgcg ggaatgggca gcaaatcgtc cagaattgca ggcgttatgt
    10861 acgcaatatg gcgagctggc gcaggcagga acacaacgat tgctgccagg gccgacgggt
    10921 gagcgcaaca cctggacgct gctgccgcgt gagcgcgtat tgtgtatcgc ggatgatgaa
    10981 caggatgcgc tgactcagct cgccgccgtg ctggcggtgg gcagccaggt gctgtggcca
    11041 gatgacgcgc tgcatcgtca gttagtgaag gcattgccat cgactgtcag cgagcgtatt
    11101 caactggcga aagcggaaaa tattaccgct caaccgtttg atgcggtgat cttccacggt
    11161 gattcggatc agcttcgcgc attgtgtgaa gccgttgccg cgcgggatgg cgcaattgtt
    11221 tcggtgcagg gttttgcccg tggcgaaagc aatatcctgc tggaacggct gtatatcgaa
    11281 cgttcgctga gtgtgaatac cgctgccgct ggcggtaacg ccagtttaat gacgatcggt
    11341 taaacggtag ccggaggatg ttacaacctc ctccggcatc tttaacgtgg tcgaatcccc
    11401 tcaataataa tccgctgcac gttttcaacc gtttgagtga aaaatatctc atcgcgcaac
    11461 gtcgcgcctg tcaccgcctc cacctgaggg gcgaaatcgg cgtaatgttg agtggaagcc
    11521 caaatcataa aaatcaaatg ctgcggatca atcggcgcga gtttgccgct tttgacccaa
    11581 ccggcaatca gcgccgattt ctcatcaatt aatgccttca aatcgcccgt cagttcatcc
    11641 attaacaacg gcgcgcctgc cagcatctcc atacaaaaca gacgtgaagc ctgcggataa
    11701 tcgcgtgaga cttccagctt aagacggatg tactctttga tcgccgccag cggggcgaaa
    11761 tcttcacgaa acgcttttaa cggtgccagc cagatatcga gaatctgccg cagcacggca
    11821 atatacagcg cctcttttga cggaaagtaa tacagcagat tggtttttga aacacccgcc
    11881 aactctgcga tctgctccag ccgtgtgccg tgaaaaccga attgtgaaaa agtgtccagt
    11941 gctgcgctaa gaatcgcttt tttcttcgcg cttactgcgc gcgaacgttt acccgttgtt
    12001 ttcactgcgc cttgcgtcat gcgctctccc ctcttgtaga ttggcaacag gatagcaaaa
    12061 cccccgcccg gcctcgacct tctgcactct catcgcgcac agatgcatgt tttatgtgca
    12121 actgttttga ccgtttagtc cactttttac cagatatttt agataacgca ttgttaacat
    12181 attaacaaca ttaacttttt aaaaactggc atccgctttg caaacaagcc aatacgcggc
    12241 tgaaaaggat atgcaggatg cagcgccccg tctgactttc actttacgag acgaagagag
    12301 gttgatgatg aaaattggcg tattcgtacc tattggcaac aacggctggc tcatttcgac
    12361 ccacgcgccg cagtacatgc cgacctttga actgaataaa gccattgtgc aaaaagcgga
    12421 gcactaccat ttcgatttcg ccctgtcgat gatcaaactg cgtggctttg gcggcaaaac
    12481 tgagttctgg gatcacaacc ttgagtcgtt caccttgatg gcggggctgg cagccgtgac
    12541 ctcgcgcatt cagatttacg cgacagctgc caccttaacg ttacctccgg caatcgtcgc
    12601 ccgtatggcc gcaaccatcg actccatctc tggcgggcgt tttggcgtca acctcgtgac
    12661 tggctggcaa aagcccgagt atgagcagat gggtatctgg cctggcgatg actatttctc
    12721 ccgtcgttac gactatctca ccgaatatgt tcaggtgctg cgcgacctgt ggggcacggg
    12781 gaaaagcgat tttaaaggcg attttttcac catgaatgat tgtcgcgtca gtccgcaacc
    12841 gagtgtcccc atgaaagtga tctgcgccgg gcaaagcgac gctggcatgg cgttctccgc
    12901 ccagtatgcc gatttcaact tctgtttcgg caaaggtgta aatacaccca cggctttcgc
    12961 cccgaccgct gtgcggatga aacaggccgc agagcaaacc gggcgcgacg ttggctctta
    13021 tgtattgttt atggtgattg ccgatgaaac cgacgatgcc gctcgcgcca aatgggaaca
    13081 ctacaaagcg ggcgcggatg aagaggcgtt aagctggcta accgaacaaa gtcagaaaga
    13141 tacccgctcc ggtactgata ccaacgtccg tcagatggcc aatcccactt cggcggtaaa
    13201 catcaatatg gggacgttag tcggttctta cgccagtgtc gcgcgcatgt tagatgaagt
    13261 cgcaagcgtg cctggtgccg aaggcgtgct gttaaccttc gatgattttc tgtcgggaat
    13321 cgaaaccttc ggcgagcgca ttcaaccact gatgcagtgc cgcgcccatc tccctgcgct
    13381 gactcaggag gtggcatgat gacgacctta accgctcgac cggaagccat taccttcgat
    13441 ccgcagcaaa gtgcgctgat cgtggtggat atgcaaaacg cttatgccac gccaggcggc
    13501 tacttagatc tcgccgggtt tgatgtctca accactcgcc cggtcattgc caacattcaa
    13561 accgccgtga ccgcagcgcg agcggcaggg atgctgatca tctggtttca aaatggctgg
    13621 gatgaacagt atgtcgaagc tggcggaccc ggctcaccga attttcataa atcgaacgcc
    13681 ctgaaaacca tgcgtaagca gccgcagctg caggggaaat tgctggcgaa aggctcctgg
    13741 gattatcaac tggtggatga actggtgccg cagcctggcg atattgtgct gccgaagccg
    13801 cgctacagcg gtttcttcaa tacgccgctg gacagcattt tgcgcagccg tggaatacgc
    13861 catctggttt tcaccggtat cgctaccaac gtctgcgtcg aatcgacgct acgcgatggc
    13921 ttttttctgg agtatttcgg cgtggtgctg gaagacgcaa cacaccaggc ggggcctgaa
    13981 tttgcacaga aagccgcgtt gttcaatatc gaaacctttt ttggctgggt cagcgacgtc
    14041 gaaacattct gcgacgcgct ttctcccacg tcctttgctc atatcgctta aggagtttaa
    14101 cgatgccaaa atccgtaatt attcccgctg gcagcagcgc accgctggcc cccttcgttc
    14161 ccggcacgct ggctgatggc gtggtgtatg tctccggtac gctggctttt gatcaacata
    14221 ataacgtgct gtttgccgat gacccaaagg cgcaaacccg ccacgttctg gaaactatcc
    14281 gcaaggtgat cgagacggcg ggtggcacga tggcggatgt gaccttcaac agcatcttta
    14341 ttaccgactg gaaaaattac gccgcgatta acgaaatcta cgccgagttt tttccgggtg
    14401 ataaaccggc gcgattctgc attcagtgcg gactggtaaa acctgacgcg ctggtggaaa
    14461 tcgccacaat tgcgcatatc gccaagtgag gccgcgatga aactttcact ctcacctccc
    14521 ccttatgctg atgcgcccgt agtggtgttg atttcgggtc ttgggggtag cggcagttac
    14581 tggttaccgc aactggcggt gctggagcag gagtatcagg tagtctgtta cgaccagcgc
    14641 ggcaccggca ataatcccga cacgctggca gaagattaca gtatcgccca gatggcagcg
    14701 gaactgcatc aggcgctggt agccgcaggg attgagcatt acgcagtggt cggccatgcg
    14761 ctcggtgcgc tggtgggaat gcagctggcg ctggattatc ccgcgtcggt aactgtgctg
    14821 atcagcgtta acggctggct acgaataaac gcccatacgc gccgctgttt tcaggttcgc
    14881 gaacgattac tgtatagcgg cggcgcgcag gcatgggtgg aagcgcagcc gttgttcctc
    14941 tatcccgccg actggatggc ggcccgcgca cctcgcctgg aggcagaaga cgcgctggca
    15001 ctggcgcatt ttcagggcaa aaataattta ctgcgtcgac ttaacgccct caaacgcgct
    15061 gactttagtc accatgcgga tcgcatccgc tgcccggtgc aaatcatctg cgccagtgat
    15121 gatctgctgg tgccaacagc atgttccagt gaacttcatg ccgccctgcc cgatagccag
    15181 aaaatggtga tgccctatgg cggacacgcc tgcaacgtga ccgatcccga aacgtttaat
    15241 gctctgttac tcaacgggct tgccagcctg ttacatcacc gtgaagccgc cctgtaagga
    15301 attgctatga acgaagccgt tagcccaggt gcgcttagca ccctgttcac cgatgcccgc
    15361 actcacaacg gctggcggga gacacccgtc agcgatgaga cgttacggga gatttatgcc
    15421 ctgatgaaat gggggccgac atcagctaac tgttctccgg cacggatcgt gtttatccgc
    15481 acggcagaag gaaaagaacg tctgcgcccg gcactttcca gcggcaatct gcaaaaaacc
    15541 ctgaccgcgc ccgtcaccgc tatcgtcgcc tgggacagtg aattttatga acggttacca
    15601 ctactgtttc cccacggtga tgcccgcagt tggtttacct ccagcccaca acttgccgaa
    15661 gaaacagcgt ttcgcaacag ttccatgcag gcggcctatc tgatcgtcgc ctgccgggcg
    15721 ctgggactgg ataccggccc gatgtcgggc tttgaccgtc aacacgtgga cgacgccttt
    15781 tttacgggca gcacgctgaa gagcaatctg ctgattaata tcggctatgg cgatagcagc
    15841 aagctttatg cgcgcctgcc acgtctgtcc tttgaagaag cctgcgggct gttgtaagga
    15901 gcaaactatg aacattgtcg atcaacaaac ttttcgcgat gcgatgtcct gcatgggcgc
    15961 ggcggtcaat attatcacca cggacggtcc agccgggcgc gccgggttca ccgccagcgc
    16021 cgtctgcagt gtgaccgata cgccgcccac attactggtg tgcctgaatc gtggggcgtc
    16081 cgtctggccg gcattcaatg aaaatcgaac gctgtgtgta aatacactta gcgccgggca
    16141 ggagccgctt tcaaaccttt ttggcggcaa aacgcccatg gaacaccgct ttgccgccgc
    16201 ccgctggcag acgggggtga ccggatgccc gcaactggaa gaggctctgg tttcgtttga
    16261 ctgccgtatc agccaggtgg tgagcgtcgg cacccacgac attctgtttt gcgccatcga
    16321 agcgattcat cgtcacacca caccctacgg gctggtgtgg ttcgatcgca gttatcacgc
    16381 gctgatgcgc cctgcttgtt aacctcctta aggagacagc tgatggcaat gttcggtttt
    16441 cctcactggc agttaaaatc gacctctaca gagagcggcg tggtcgcgcc ggatgaacga
    16501 ttgccgtttg ctcagacggc agtaatgggc gttcaacacg cggtggcgat gtttggcgcg
    16561 acggtgttaa tgccgattct gatggggctg gatcccaatc tttccatttt aatgtcgggg
    16621 atcggcacgt tactgttttt ctttattacc ggcgggcgcg tccccagtta tctcggctcc
    16681 agcgcggctt ttgtcggcgt ggtgatcgcc gcgacgggct ttaacggtca gggcattaac
    16741 ccgaatatca gcattgcgct gggcgggatt atcgcctgtg ggctggttta taccgtcatt
    16801 ggcctggtgg tgatgaaaat cggtacgcgc tggattgaac gactgatgcc gccggtggtg
    16861 acgggtgcgg tggtgatggc gattggcctg aacttagcgc ctatcgcagt gaaaagtgta
    16921 tcggcttcgg cctttgatag ctggatggcg gtaatgacag tgctgtgtat tgggctggtg
    16981 gcggtgttta cccgtggcat gatccagcgg ttattgattc tcgtaggatt aattgtcgcc
    17041 tgtctgctgt atggcgtgat gaccaacgtt ttagggctgg gaaaagccgt cgattatact
    17101 ctcgtcagcc atgctgcctg gttcggtctg ccacattttt ccactcctgc ttttaatggt
    17161 caggcaatga tgctgattgc gcccgtggcg gtgattctgg tggcggaaaa cttaggtcat
    17221 ctcaaggcag tcgccgggat gaccggacgc aatatggatc catatatggg acgggcattc
    17281 gtcggtgatg ggctggcgac gatgctttct ggttctgtcg gtggcagcgg cgtaaccacc
    17341 tacgccgaga atatcggtgt aatggccgtg acaaaagtct actcgacgct ggtgtttgtc
    17401 gctgctgccg tcatcgccat gctgttgggc ttttcaccga aatttggcgc gctgattcat
    17461 accattccgg ccgcggtgat tggtggtgca tcaattgttg tgttcgggct gattgccgtc
    17521 gcaggcgcaa ggatctgggt acaaaaccgt gtcgatttaa gccagaacgg taatttgatt
    17581 atggtcgcag taaccttagt gctgggcgcg ggtgattttg ctctcacgct gggcggtttt
    17641 acgttgggag ggattggtac agcaaccttt ggcgcgattt tactcaatgc gttattaagc
    17701 agaaggttgg ttgacgttcc accgccagaa gttgttcatc aggagccata aaaagaaaac
    17761 cgccgggatt acccggcggt gcagattgtt gacaacgtac gcattgttca tgccggatgc
    17821 ggcgtaaacg ccttatccgg cctacaaaac agtgcaaatt caatatattg caatcttctg
    17881 gtaggcctga taagcatcgc gcatcagaca agcttacatt tgtcatcagt atcaaccacc
    17941 gggtaaccgg cggtgatggc tttttgacga gctacagcca aattgcattc gtcactgatt
    18001 agtccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta cctgcttcag
    18061 atgcgcgctg cgggtcattc ttgaaattac ccccgctgtg ctgtccacct tttttacctg
    18121 cttctgatgc tctttcgcgg tcttctgcaa aattgccgga accgcctcga tggtttgcca
    18181 ttactgacct ccgtctgggt atagacatca tattgcatag aattaacgcg gcgtatggcg
    18241 gctgccatat tttcgctacg tgggactaag cttagtgaac tgacgcgggc agacccgcgt
    18301 ttggtaatat cctgcaacag cttcttagcg agaatagata tcatctccaa taattatcca
    18361 taagccgctc ttatgattaa aaaaacgcga tgagataaaa atgtgtcatt ttgcgacaaa
    18421 attacgtgct tgtgaaagtt gttataaatc aaataagtgg ttgtgaaatt tgcactctga
    18481 aaaggacgtc ttatctttaa ataagtggta gcgaatcgct acggaataga gataacacga
    18541 ggagtggtta gaaatggcta aagttctggt gctttattat tccatgtacg gacatattga
    18601 aacgatggca cgcgcagtcg ctgagggtgc aagcaaagtg gatggcgcag aagttgtcgt
    18661 taagcgtgta ccggaaacca tgccgccgca attatttgaa aaagcaggcg gtaaaacgca
    18721 aactgcaccg gttgcaaccc cgcaagaact ggccgattac gacgccatta tttttggtac
    18781 acctacccgc tttggcaaca tgtccggtca aatgcgtacc ttcctcgacc agacgggcgg
    18841 cctgtgggct tccggcgcac tatacggaaa actggcgagc gtctttagtt ccaccggtac
    18901 tggcggcggt caggaacaaa ctattacttc aacctggacg acccttgcgc atcacggcat
    18961 ggtaattgtc cccattggct acgcagcgca ggaattattt gacgtttcac aggttcgcgg
    19021 cggtacgccg tacggcgcaa ccaccatcgc aggcggtgac ggctcacgcc agccaagcca
    19081 ggaagaactg tctattgctc gttatcaagg ggaatatgtc gcaggtctgg cagttaaact
    19141 taacggctaa tcttcaacag gaggatacgc atgccaactc aagaagcgaa agctcatcac
    19201 gtcggtgaat gggcatctct gcgcaatacg tcgccggaaa tagccgaggc cattttcgaa
    19261 gtcgccgggt acgacgaaaa aatggcggaa aagatttggg aagaaggtag cgatgaagtg
    19321 ttagtcaaag cctttgctaa gaccgataaa gattcgcttt tttggggcga acagaccatc
    19381 gaacgtaaaa acgtttaacc agcgactccc ccgcttctcg cgggggagct ttctgttatt
    19441 tcaccgcttc attcaacacg ctatcaaatt tatccatcgg gcagaaaccg ttggcatcta
    19501 tcgggcaacc gcttaattcc agcgtcacac gctgagcagg tgcctgcagg gttaacgcat
    19561 cggcattacg taactgttcc gcactctgat acacatattc aattttcatc aaatcgcgat
    19621 tggctttgct gtcatgccaa cgctggaaaa cgattttgcc gccaatcggc gtgcgttcgt
    19681 tctggtcatg caactgatac ggtttgaaat ccagcgccgt taacagagag gcaatgttgg
    19741 agtcgtgccc aactaacacc gtgattttcg gtgcacgggt gcgatcggtg accagagctt
    19801 tgtcgatata actgaccagc ggtttcgcaa cattgcgcgc cacttccggt gaggtaaaca
    19861 ggctgtcctg gtaaccgttt ttcagcttcg acaacacctt ccactgctgg tcagatttga
    19921 tttctcccca ggccacctga tccatcggaa aaccttcgta gtattgcaaa gtaaacgcat
    19981 ctaccagcga gttgccgact ttcagcggcc cggaaacacc tggttcttgt tgatacttcg
    20041 cgctaaaggt atttttgcca tccaccagcg aacactgttg tttctcttta caggcagggg
    20101 aatctttata gttaacgatt ttttccagta gctggtagct gtcggtaagc tggagtttgc
    20161 tgagctcttt ctccattgcc gccaccgcct gttcactgaa tgcggcggaa tcatcggtga
    20221 tcaccgggtt aaaggttggg tccatggtgc ccattttttc ctggtgatgc acaggaatat
    20281 cacaccccgg gaatgcgccg gtaataaaga actgtgcggt cgcaacggta cgttgcagac
    20341 tattggcata ggcataaacg gtgtctggcg gcgggcattc ccccgatttc accatcccct
    20401 gctctgccag ccattcacgc atgtaatgcc ccatatacac ttcgagtacg ccacctttgg
    20461 tggtgagttg cccaccgggg acgtcccatt cgggccattt attcggcgtc gactgctcca
    20521 gcacactgcc attgttcgcc agcggcgcac gtaagttatg gcggctcatc atgagcactt
    20581 gctgtagctg atagccttcc ggtacggttt gtgcctgagc atttgaagcg agtaatacta
    20641 tccctgccac agttgcggcg attagcgttt tgttcattcc tggcacctct tttgttatca
    20701 atcactaaag agtgtgacag aaatatgaca tttttccggg aacaggttcg caaaatggct
    20761 tgttgaatgc ctgtttttct atatacatct ttaaaaacag gcaaggtttg acgcaaacta
    20821 tgcctgatta gctccctgcc tcgcgtacgc cgctgatgat tacacctgtg gctaaacaga
    20881 atatcagcgg cgttactaat acagaggcaa tagtaccaat aataagagaa gtgaggctaa
    20941 taacaatggt ttttagcaca tacctttgtc attaaattgc ataacgattt aaatagtcga
    21001 gttgcgcatt catttcctta tttcttaact tataagcctg agcaaaaag
//
LOCUS       DAJOSY010000079        20580 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJOSY010000079 DAJOSY010000000
VERSION     DAJOSY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.079"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1286)
                     /locus_tag="OHN27_003048"
     CDS             complement(<1..1286)
                     /locus_tag="OHN27_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCQ2287442.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLG"
     gene            complement(1298..1585)
                     /gene="eutN"
                     /locus_tag="OHN27_003049"
     CDS             complement(1298..1585)
                     /gene="eutN"
                     /locus_tag="OHN27_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCQ2287443.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(1692..1985)
                     /gene="eutM"
                     /locus_tag="OHN27_003050"
     CDS             complement(1692..1985)
                     /gene="eutM"
                     /locus_tag="OHN27_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCQ2287444.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(2024..3040)
                     /gene="pta"
                     /locus_tag="OHN27_003051"
     CDS             complement(2024..3040)
                     /gene="pta"
                     /locus_tag="OHN27_003051"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCQ2287445.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(3030..3839)
                     /gene="eutT"
                     /locus_tag="OHN27_003052"
     CDS             complement(3030..3839)
                     /gene="eutT"
                     /locus_tag="OHN27_003052"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCQ2287446.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFCAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGERNDY"
     gene            complement(3836..4537)
                     /gene="eutQ"
                     /locus_tag="OHN27_003053"
     CDS             complement(3836..4537)
                     /gene="eutQ"
                     /locus_tag="OHN27_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCQ2287447.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESSPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWDNAFFPWTLNYDEIDMVLEGELHVRHAGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(4512..4991)
                     /gene="eutP"
                     /locus_tag="OHN27_003054"
     CDS             complement(4512..4991)
                     /gene="eutP"
                     /locus_tag="OHN27_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCQ2287448.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(5004..5339)
                     /gene="eutS"
                     /locus_tag="OHN27_003055"
     CDS             complement(5004..5339)
                     /gene="eutS"
                     /locus_tag="OHN27_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCQ2287449.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(5632..7911)
                     /gene="maeB"
                     /locus_tag="OHN27_003056"
     CDS             complement(5632..7911)
                     /gene="maeB"
                     /locus_tag="OHN27_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCQ2287450.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGYANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            8200..9150
                     /gene="tal"
                     /locus_tag="OHN27_003057"
     CDS             8200..9150
                     /gene="tal"
                     /locus_tag="OHN27_003057"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCQ2287451.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            9170..11173
                     /gene="tkt"
                     /locus_tag="OHN27_003058"
     CDS             9170..11173
                     /gene="tkt"
                     /locus_tag="OHN27_003058"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCQ2287452.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(11268..12311)
                     /locus_tag="OHN27_003059"
     CDS             complement(11268..12311)
                     /locus_tag="OHN27_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCQ2287453.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGDQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(12437..13012)
                     /gene="nudK"
                     /locus_tag="OHN27_003060"
     CDS             complement(12437..13012)
                     /gene="nudK"
                     /locus_tag="OHN27_003060"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCQ2287454.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNTKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(13080..15059)
                     /gene="aegA"
                     /locus_tag="OHN27_003061"
     CDS             complement(13080..15059)
                     /gene="aegA"
                     /locus_tag="OHN27_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCQ2287455.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     LIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            15265..16965
                     /gene="narQ"
                     /locus_tag="OHN27_003062"
     CDS             15265..16965
                     /gene="narQ"
                     /locus_tag="OHN27_003062"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCQ2287456.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTNLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            17129..20242
                     /gene="acrD"
                     /locus_tag="OHN27_003063"
     CDS             17129..20242
                     /gene="acrD"
                     /locus_tag="OHN27_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCQ2287457.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDDLAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(20341..20400)
                     /gene="ypfM"
                     /locus_tag="OHN27_003064"
     CDS             complement(20341..20400)
                     /gene="ypfM"
                     /locus_tag="OHN27_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCQ2287458.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         4698 a         5619 c         5368 g         4895 t
ORIGIN      
        1 cccagcccca gcccggcaat gcacggtccg ttcttaacga aaatgctggt atcaatagca
       61 ttcgccatct ggttcatgtt ttcgatgttg cgcgagtgca ttgccgccgt gtggtggcaa
      121 ccgccttcca gtttcaccgc cagcgcaatg gcatccgcca cgttggcgac gcgcacgacg
      181 ggcagcaccg gcatcatcag ttcggtcacg gcaaacggat gttctgcagt ggtttccaca
      241 aacagcaaac gcgtttcttg cggcacgttc aggccgattg ccgccgcgat tttcgccgca
      301 tcgcgaccaa cccagtcacg gctgacggtg ccttttccgc gctcgtcgat atttttcagc
      361 aacaccggtt gcagctgctg cgcctgttct gcggtcagtt tcaccgcgtg ctggccttcc
      421 atcagacgca tcagttcatc ggctacgcta tcaacaacaa tcagtacctt ttcgtcggca
      481 caaatgatgt tgttatcgaa agaagcgcct ttgacgatgg actgagcggc acgggcgagg
      541 tcggcggttt catccaccac taccggcggg ttgccagcgc ctgcggcaat cagacgttta
      601 ttggtgtgtt tacgcgccgc ttctactacc gcttcgccgc cggtcaccac cagaaggccg
      661 atgcccggaa acttgaacaa acgctgcgcg gtttcgatat ccggatttgc cacagtaacc
      721 agtaagtttt ccggcccgcc tgcggcaaca atcgcctggt tgagcagcgt gatcgcccgc
      781 tgggagactt ttttcgccgc cggatgcggg gcaaaaatga cgctgttgcc cgcggcaatc
      841 aggctgatgg cgttattaat tacggttgcc gccgggttag tggacggagt taccgaagcc
      901 accacgcccc agggcgcgtt ttcaattaaa gtcaggccgt tgtcgccggt cagcacctgc
      961 ggagagagac actcaacgcc tggcgtgccg cgcgcctgag cgacgttttt ggcaaattta
     1021 tcttcaacgc ggcccatgcc ggtttcactg acggcaagtt ccgctaaatc tctggcgtgt
     1081 ttttcgcctg cctcacgaat ggcagcaatg gctaactggc gcattgccac gctttttaac
     1141 ccttgctggg cgactttggc tgccgcaacg gcgtcatcca gggacgcgaa aacgcccatc
     1201 tcatgaacgg cggcgggcgg cgtgtcactg ctttgcattt tcagcagtac cgctttcacc
     1261 acctgttcaa tatcctgttg attcatgatg ttctgcctta tttgtggaaa attacctgac
     1321 cgccagacac cacctcatcg acaatgccaa tcacgcacag atcgaccggt gacgtttcgc
     1381 ttttatgcgc ctggcgggcg gaactgccac tcaccagtaa cacccactcc ccggttcccg
     1441 cgccaatatt gtcgatggcg acggcgcatt gcccgtcagg attaccttgt ggatcaatca
     1501 tttccaccat cagcaatttg tcatgcgcca gtccgtgatg gcgtacggta caaacaattt
     1561 gtccagtgac gactgccagt ttcatacccg cctccgtggc gtatttcagg taaaagctcc
     1621 ccctaccctc cgcagaaggt aaaatgaaaa aggagagagc gtgacgcccg aatcgacgtc
     1681 acacagggtg attacaggtt gctgctatcg cctttcaggc cgatcgggaa gacttcttcc
     1741 aggtcgccgt gcggacgcgg gataacgtgt acagatacca gctcgccgat acgctgtgcc
     1801 gctgcggcac ctgcatcggt cgcggcttta caggctgcaa catcaccacg aaccatggca
     1861 gtacacaggc cgccgccaat ctgcttaaca ccaaccagct tgacgcgcgc agctttcacc
     1921 atcgcgtcag aagcctcaat cagtgcaacc aggccccggg tttcgatcat tcctaatgct
     1981 tccattgtgc tttcctcttt atcagggtcc agaacgggac cgttcattca accagtgttt
     2041 gtaaactgct ttcgcggttc acttctgtct gacgcggcac agctgccacc agcgccagct
     2101 cgataatttc ctgcacgcta caaccacgag agagatcgtg catcggcgcg gcaagtcctt
     2161 gtatcagtgg cccgacggca cgatatccgc cgagtcgttg tgcgattttg taaccaatat
     2221 ttccggcttc cagcgacgga aaaaccatca cattggcctt gccctgtagc gggctggcag
     2281 gcgctttttg cgccgccact tccggcacga aggcggcgtc aaactgtaac tcgccatcca
     2341 ccaccagctt tggtgcgcgc tcacggacga tttctgtcgc ctgctggacg ttagcaacac
     2401 aggggtgacg ggcgctacca ttgctggaaa acgacagcat cgccacgcgc ggctcttctc
     2461 cggtgatggc gcgccaggtt tcggcactgg caagcgcgat atccgccagc tgcgccgccg
     2521 tcggctgtgg caccacgctg caatcggcaa agcccaacgc cgggccgctg tactgtggca
     2581 acatcaggaa aatggaagag agcgttttac agcctggctg caagccgata atgcgtaatc
     2641 cggcacgcag cacatttgcc gtggaagaga ggttgcccgc gatacagaca tccgctttac
     2701 cggcgctgac cattgctgcg gcgaacatca gagggtcggt aagtttttcc agcgcatccg
     2761 gcggcgtttt ttcgcccgcg cgggccagcc agcgatgagc aaattcttcc cgcattgcga
     2821 ggttgccatg cggatcaatc acctgtagcc cgtccatcgc cacgccgtga ctgagcgcaa
     2881 actgacgaag ttcaaacgga ttggcgacca gaatgggcgt tgccagacct tgttgatgta
     2941 aatattgcgc ggctttcagc acacgttggt ctaacgcatc cggaaaaacc actctggcgg
     3001 gcgctcgcaa cgccagttca cgacaacgtt caataatcat tgcgttcccc cagtcgttgt
     3061 tggatctgct tcaccgttag cggctgcttc gttacgctca ggatcatcat cacgtagacc
     3121 gtgctggaaa ggcggttcag cgcctgcaaa atgtccgggc gtaatacttc aaagctgcgg
     3181 gtgataaaga cctgcgccgc tacggtttcg gtttcccgca ctttggtgcg tagcagattg
     3241 agcaacgcag catcgcgccc gtggctggct tccggcacca gatgatcgtg gtcgagatag
     3301 cgcagcggct gatgagaaag ccgatgcaga tcttcgtcag aaagcccgac aatcgcctgg
     3361 cagcccagcg gttcccccag cgcatcggcg cgcataatgt tgccaagacg cgaacggata
     3421 tccgccagcc acggctgcca cggttccgcc agttcaattt gcagccacac tgccagcgca
     3481 atggtgctgt cgagaaccgc gcaaaaaccc agacgcggat cgcttttggc gaccattttt
     3541 tccgccgaca ggtgagtcag cgtatccggc ttttttgcca ccggctggcg gcacagttcg
     3601 cagcacgcct gcggatgttc atcgctactg gtcagcccat gaacgggctg cggctgctgc
     3661 tgttcatcgt caacaaacag gcggccctgc tcgtcaataa acttgatgcg cagatggcgg
     3721 ctttccagca actcccgggc agagggcgtc aggcgactgt ccgcagggag atggatctct
     3781 gcgccttcgc tgagcgtatg gttcgctctt agccatgctt cggtgatgaa atctttcata
     3841 gggattgcca gttagccggc caggcgacat acaggaattt cacgctggat gtcgtaccaa
     3901 attcgatgct ggagcctttc gggataaaca tcacatcgcc cgctttggcg atcatggttt
     3961 ctcctgcgtg gcgaacatgc agctcccctt ccagcaccat atcgatttcg tcgtagttca
     4021 gggtccacgg gaagaatgcg ttgtcccact gcataaaacc ggcggccatg ctgctgccgt
     4081 cgtctccggt cacaaggtca gttaagccca cgcaatgcgg ctctgcgccg tcgaaacgac
     4141 caaacttgac gctgctacca tcaatcactt tgatgccgcc tttgccggtc accgatttga
     4201 agctcggctg cattgcaccc tgctccagcg actgtttttc cttcatcact ttttccatca
     4261 actgcgcgac caggctttcg gtaaactggc cttccggcag ctgggcaatg atggtttcgc
     4321 ggatgcgctg actttcggtt ttatcggctg gcacgctggc gggaacggac gccgttaccg
     4381 gggacgattc atcacattcg gtgatggtaa aacccagtag atccgccact tcacgggctt
     4441 ccggggtaat aatgctggcg cgcagaacca cggacattgc ctgttcgccg cgcgcgtgtg
     4501 cttcacgaat atcattcgct gtgatgagtt ttttcacctg cttcctcctg tttggtgaga
     4561 gatgccagat aatccaccag ttgctgcacg ctttgcgggt cgtggctgtt tagctcgaaa
     4621 atcggctctt cgaagccagt ctcaagcaat aactttcgcg ttgcggcgac gtcagcatct
     4681 ggcatgtccg ttttactgat gacggcgatt tgccgtttgc tgacgccaat atccagcaac
     4741 ccggcaggca accgactttc cgggtcattc gcgccgtgga catagatcag catgtccaca
     4801 tcctgtagcg tggtaattaa ggcgtgatac cagcggggat ggctaaaata ttcacccggc
     4861 gtatcaatat cgcccttatc attaaattcc acggcctgtg tttttctggc gagggtataa
     4921 ttcccctgca acgcattaaa taacgttgtt tttcctgccc cgacggtacc cacaaacgca
     4981 attcgtttca tgaatgcctc gtattaactt ttggtcattt cacaaagcgt ataatttaat
     5041 aagcgaccta aaccgctgac ggtttgtgat aacgcttctt ctactgcgcc caccgagcca
     5101 taaatcacca atgcaccact gaagcggtcg agaaaaccga tatgcacatc agcggctttc
     5161 aatgctaaat caccggcgat catcgccgtt tcgccaggag ttagcgtcat aatgccaatt
     5221 gcgcccgcat ccggaacgcc gattttcttc gccagttcct cgccgggatg cgcgatgaga
     5281 tgcgccagcg tgacctgttt gccgggcaca aattcctgaa tgatgcgttc tttatccatg
     5341 agtcgccgcc tgtgaaaaac tttcgtgtat cgtgacaatt tttcgtcaga gtaaataaca
     5401 aaattcggca agcgcgttta aaaggtgagg tagatcacta agaatggaga gatattcgct
     5461 gtggtgcata aactttcata tgcaacgcat gaatataaat aaaataatat cgagatatta
     5521 tatagactgc ttttttttat tattcgcaaa ctcagtaata agctttacta atatattgca
     5581 gctaaactgc ttaccctgaa tattcaggat aagcgtgaga gttaaaaaaa attacagcgg
     5641 ttgggtttgc gcttctacca cggccagcgc caccatgttg acgatacgac gtaccgatgc
     5701 gatcggcgtt aacacgtgaa ccggtttcgc cacacccatc agcaccgggc cgacagtcac
     5761 accttccgag ctggaaacac gcagtaagtt gtaactaatg cgggcagctt ccatgttcgg
     5821 catcaccaga atattggcgt aacctttcaa agggctgtcc ggcatacggt cgttgcgaat
     5881 cgcttccacc agcgctgcat cgccgtgcat ttcaccatca atcatcagtt ctggtgcacg
     5941 ttccctgacc agttccagcg cctgacgcat tttgctcgac gacgggcagt cagaagaacc
     6001 aaagttggag tgcgacaaca aagcaacgcg cggctcaata ccaaaacgac ggacagtttc
     6061 tgccgccatc aaggtgatct ccgccagctc ttctgcatcc ggttcatcat taacatatgt
     6121 atcggcaata aaggtgttac cactcggcag cagcagcgcg ttcatggcac ctgcggtgtg
     6181 aacgccatcg cgataaccaa agacattttt caccacgcta aaatgttcat gataatcacc
     6241 caccgtaccg caaatcattg catcggcttc cccacgctga accatgatcg cgccgatcac
     6301 tgtcgggtta ctgatcagcg cccgctgcgc ctgttcctga gtgacgccgc gacgcttcat
     6361 gatctggaag tattcggtcc agtactcttt aaagcgcgga tcggattcgt tattgacgat
     6421 ctcaaaatca acgcccgctt tgatctgcaa gcccagtttc tgaatgcgca tttcgatcac
     6481 gttcggacga ccgataagga tcggtttcgc cagtcccagc gttaccagtt cctgagtggc
     6541 atgcagaacg cgcgcctctt ccccttccgg cagaacaacg cgcttcggcg ctttgcgagc
     6601 ctgggagaaa atcggcttca taaacaggtt ggttttgtaa acgaactcag tcagcttgtc
     6661 gatgtagacg tcgaaatcag caatcggacg agtcgccacg cccgactcca tcgcggcttt
     6721 agcgaccgca ggagcgatct taacgatcaa gcgcggatca aacggttttg gaatgatgta
     6781 ttccggacca aagctcagat cctgatcgcc atacgctgaa gccaccactt cgctctgttc
     6841 cgcatgggcg agttctgcaa tcgcacgtac cgccgccagt ttcatctctt cgttgatggc
     6901 ggttgcgcca acgtccagcg cgccacggaa gatgaacggg aagcacagga cgttgttcac
     6961 ctggttcgga tagtcagaac gaccggtgca aatgatggca tccggacgca cttctttcgc
     7021 cagcggcggc agaatttccg gttccgggtt cgccagcgcc aggatcattg gcgcacgagc
     7081 cattttcttc accatttcct gggtcagcac tttcgggccg gaacagccca ggaaaatatc
     7141 cgcgccttca atcacatcat cgagggtacg tttgccgtca tccaccaccg catatgcggc
     7201 tttggtttcc gccatgtttg gctcacggcc ctgatagata acgccttttg aatcgcaaac
     7261 cacgatgtta tgtttttgca gacccagcgc taccagcagg ttcatacagg cgattgctgc
     7321 ggcacccgcg ccggaaacca ccatccgcac gtcggagatg tttttctcca ccacgcgcaa
     7381 gccgttgagg atggcggcag tgctgataat tgccgtgccg tgctgatcgt cgtggaatac
     7441 cggaatattc atccgctcgc gcagtttctg ttcaatatag aaacattctg gcgctttaat
     7501 gtcttcgagg ttgatgccgc cgaaggttgg ttcgagcgcg gcgacaactt caataaattt
     7561 gtccgggtcg agttcgtcaa cttcaatgtc aaatacatca atcccggcga atttcttaaa
     7621 cagaacgccc ttgccttcca tcaccggttt gcctgccagc gcgccaatgt tgcctaaccc
     7681 cagcaccgcc gtaccgttag agatcaccgc caccaggtta ccgcgggcgg tatatttgta
     7741 ggcttttaac gggtcttttt cgatttcaag acaaggtgcg gcaacgcctg gtgagtaggc
     7801 cagcgccaga tcgcgctgtg ttgccagagg cttggttgga gaaacctgga ttttccctgg
     7861 aactggaaat tcatggaaat caagtgcact ttgttttaac tggtcatcca tttggttgtt
     7921 cctttcacgt aacgttcaca aataaagtgt gtgggcaaca gcccctgccc acaacgtggc
     7981 gcacattatt accctgccgg agtctacaga ctttgagcaa gtccaaactc tcaccattaa
     8041 tataatgttt tggtaataat cctataacac tgatgttacc tgcttaatcc agcaatacca
     8101 tgcctgtctg ctatgctttt ttgatgcgtt tagcgaaatt tctcagaagt gtgaattaac
     8161 gcactcatct aacactttac ttttcaagga gtatttccta tgaacgagtt agacggcatc
     8221 aaacagttca ccactgtcgt ggcagacagc ggcgatattg agtccattcg ccattatcat
     8281 ccccaggatg ccaccaccaa tccttcgctg ttactcaaag ctgccggatt atcacaatat
     8341 gagcatttaa tagacgatgc tatcgcctgg ggtaaaaaaa atggcaagac ccaggaacaa
     8401 caggtggtcg cagcgtgtga caaactggcg gtcaatttcg gtgctgaaat cctcaaaatc
     8461 gtacccggtc gcgtgtcaac agaagttgat gcacgcctct cttttgataa agaaaagagt
     8521 attgagaagg cgcgccatct ggtggacttg tatcagcaac aaggcgttga gaaatcacgc
     8581 attctgatca agctggcttc gacctgggaa ggaattcgcg cggcagaaga gctggaaaaa
     8641 gaaggtatta actgcaacct gacgctgctg ttttcttttg cacaggcacg ggcctgtgcg
     8701 gaagcaggcg tttttctgat ttcgccgttt gtcgggcgta tttatgactg gtatcaggca
     8761 cgcaagccga tggacccgta tgtggtggaa gaagatccgg gcgttaaatc ggtgcgcaat
     8821 atctacgact actataagca acaccactat gaaaccattg tgatgggcgc gagcttccgt
     8881 cgcaccgaac aaatcctcgc cttaaccggc tgcgatcgac tgactatcgc accgaatttg
     8941 ctgaaggagc tgcaggaaaa agtttcgcca gtggtacgta aattaatccc accttctcag
     9001 acgttcccac gcccagctcc catgagcgaa gcggagttcc gttgggagca caatcaggat
     9061 gcgatggcgg tagaaaaact gtctgaaggc attcgtctgt tcgccgttga tcaacgcaaa
     9121 ctggaagatc ttcttgccgc caaactataa accagccacg gagtgttata tgtcccgaaa
     9181 agaccttgcc aatgcgattc gcgcactcag tatggatgcg gtacaaaaag ccaactctgg
     9241 tcatcccggc gcgccgatgg gcatggctga tattgccgaa gtgctgtgga acgattttct
     9301 taaacataac cctaccgacc caacctggta tgatcgcgac cgctttattc tttccaacgg
     9361 tcacgcgtcg atgctgctct acagtttgct acatctgacc ggttacgacc tgccgctgga
     9421 agaactgaag aacttccgtc agttgcattc gaaaacccca ggccacccgg agattggcta
     9481 tacgccaggc gttgaaacca ccaccggccc gcttggacaa ggtttggcga acgccgtcgg
     9541 gctggcgata gcagagcgta cactggcggc gcagtttaac cagccagacc atgagatcgt
     9601 cgatcacttc acctatgtgt ttatgggcga cggctgcctg atggaaggta tttcccacga
     9661 agtctgttcg ctggcaggca cgctgggact gggcaagctg attggttttt acgatcacaa
     9721 cggtatttcc atcgacggtg aaacagaagg ctggtttacc gacgatacgg caaaacgttt
     9781 tgaagcctat cactggcatg tgatccatga aatcgacggt cacgatccgc aggcggtgaa
     9841 ggaagcgatc cttgaagcgc aaagcgtgaa agataagccg tcgctgatta tctgccgtac
     9901 ggtgattggc tttggttcgc cgaataaagc aggtaaggaa gaggcgcacg gcgcaccact
     9961 gggggaagaa gaagtggcgc tggcacggca aaaactgggc tggcaccatc cgccatttga
    10021 gatccctaaa gagatttatc acgcctggga tgcccgtgaa aaaggcgaaa aagcgcagca
    10081 gagctggaat gagaagtttg ccgcctataa aaaggctcat ccgcaactgg cagaagagtt
    10141 tacccgacgg atgagcggtg gtttaccgaa ggactgggag aaaacgactc agaaatatat
    10201 caatgagtta caggcaaatc cggcgaaaat cgctacccgt aaggcttcgc aaaatacgct
    10261 taacgcttac gggccgatgc tgcctgagtt gctcggcggt tcggcggatc tggctcccag
    10321 caacctgacc atctggaaag gttctgtttc gctgaaggaa gatccagcgg gcaactacat
    10381 tcactacggg gtgcgtgaat ttggcatgac cgctatcgcc aacggcatcg cgcaccacgg
    10441 cggctttgtg ccgtataccg cgacgttcct gatgtttgtt gaatacgccc gtaacgccgc
    10501 gcggatggcg gcactgatga aagcgcggca gattatggtt tatacccacg actcaattgg
    10561 cctgggcgaa gatggtccga cgcaccaggc tgttgagcaa ctggccagcc tgcgcttaac
    10621 gccaaatttc agcacctggc gaccgtgcga tcaggtggaa gcggcggtgg gctggaagct
    10681 ggcggttgag cgccacaacg gaccgacggc actgatcctc tcaaggcaga atctggccca
    10741 ggtggaacgt acgccggatc aggttaaaga gattgctcgt ggcggttatg tgctgaaaga
    10801 cagcggcggt aagccagata ttattctgat tgccaccggt tcagagatgg aaattaccct
    10861 gcaagcggca gagaaattag caggagaagg tcgcaatgta cgcgtagttt ccctgccctc
    10921 gaccgatatt ttcgacgccc aggatgagga atatcgggag tcggtgttgc cttctaacgt
    10981 tgcggctcgc gtggcggtgg aagcaggtat tgccgattac tggtacaagt atgttggtct
    11041 gaaaggggca attgtcggga tgacgggtta cggggaatct gctccggcgg ataagctgtt
    11101 cccgttcttt ggctttaccg ccgagaatat tgtggcaaaa gcgcataagg tgctgggagt
    11161 gaaaggtgcc tgatggtgat tgccggatgc tgattgccgg atgcgacgct gacgcgtctt
    11221 atccggccta cagcgtctta tccggcctac atgtcccgcc attttgttta acgggtgatc
    11281 cacaacgtgg gccaggcgtc tggcccatgc cagttatcgc aggtgggttc tgcggcgtaa
    11341 cgcaccaggc gaaaacgctg accgtcaaag cgccagcgcg cctgaatgcc acaatcgctt
    11401 aatccgcgcc ctttcgctaa ggtcaccagt tcacgcgatt tctcatcaaa tgttgcgttc
    11461 atcagttcca gttcattcgt ctcctgaccg ttgttgaacg gcaaacgcaa ccgaaccggg
    11521 cgcgaagcta gtggcttttt acgcgacaca atccatgcca aatcaatggt gttataggcc
    11581 cctgcctcac agctaatcat catcagcgct ttatcatcag tcagcgcagt gacattcacc
    11641 tcacgacgca atggatcaag cgagcagcgc agaccattca tccgccagtt tccataatcc
    11701 agcaaatcgt tgcgttcttc gagtgagagt ggcgtcggcg ttgggttaac caccgcgacc
    11761 tctttcagcg caggcgcggg cggtacgctg agcggcggtt cgtccccttt cttgatccac
    11821 gcggtttcac tgccaacgcg cttttgctga gcatcaataa acaacaacgc tgcttttaag
    11881 ccactcagag aaatggtctg atcgccatcg cgtaaggtga ttgccttccc ttcctgaatc
    11941 atctgcaaaa acgcggtgat ggttgccgta tcatcggtta ctaataacca tggtgaaatc
    12001 cgccacttgt caccacttaa cgccaacggc tcgccatcta acagcagccg tggcgctatc
    12061 tccccttctg acgcctccgg cgacttcaat ccgccgcgct caatacgtaa aacggcatcg
    12121 gtatgcgccc cggcgctgcg gctcagggtc atcaccagtc cattatgatc gcccgtatta
    12181 cgcgccacgc agaaattttg gttattacag gtgacctgcc agtcggaaaa cgcccgttgc
    12241 gctggtgccg cccacaccaa agacgtcggc aacaaagcga aaaaaaagag aaggaaaatg
    12301 cgatagcgca tggacggtac gaccccagaa gttcaaacaa acagcacaag tcgtatcttc
    12361 gttgcgcagg tagcgcggct caatcggatt tgtcggattg agccaataat aaacaacgaa
    12421 atattattgt tatttttcag tccattaaat gtgacgtttg caaatagtta agcaataaca
    12481 ccgtcttacc gtcacgtatc tcgccggttt tgatcatctc cagcgcctgg ctgaacggca
    12541 gctcgagcac ttcaatatct tcatcttcga caccgccacc ggcgttagcg cgctgattgt
    12601 cactgtattc ggcgataaaa aagtggatta gctcagtcac accgcctggc gacatataca
    12661 gttcaaataa tttgcgcact tcacccactt catagcccgt ctcttcaatc gcttctttgc
    12721 gaatacacac ttccggttcg tcgttatcca gcagcccggc gcaggtttca atcagctgcc
    12781 cgctttcatt gccattaacc caggtagcga cacggaactg acgaatcaga accacggtct
    12841 ttttcttcgt gttgtacagg aggatcgtcg caccattgcc gcgatcgtac acttcacgtt
    12901 tatggcggat aacttcgccg tctttgcgcg tgagatcgta agtaatgttg tgcagggtga
    12961 aatagttatc ggagagaatt ttgtctttaa tgagggtgat ttgttgcgtc ataccgactc
    13021 cacagcgcga aatgaacaat tatcttacgc tgtgaagtcg ggtttgtctg cgcaggctat
    13081 cagtgagatt tgactgattt tacccccagc cagtcaataa tcccctgtgc cgcatgacgt
    13141 ccttctgcca ttgcggtaac caccagatcc gcaccacgca cggcgtcacc accagcgaag
    13201 attttcggat tggtggtctg gtaacggtac tggctttcca catccgcgat gatgcggccc
    13261 catttgtcta ccgttacacc gtgcgactcc agccacggca tcccgtgcgg attgaagcca
    13321 aacgccataa tcaccgcgtc ggctggcatg acaaattcac tgccttccac cggcactgga
    13381 cgccgacgcc cctgggcatc cggctcgcca agacgcgtgc gcaggaaacg aatcccgcag
    13441 acgtgacctt gttcattcag ctcaagcgcc accggctgga cgttaaattc gaagttggcc
    13501 ccctcttcgc gggcgttctt cacttctttc ttcgagcctg gcatgttagc ttcatcacga
    13561 cgataagcgc aggtgacgtt actcgcgccg tggcgcagtg cggtacgcac acagtccatc
    13621 gcggtgtcgc cgccgcccag taccacgacg ttaagtccgg cggtattgat aaacggctct
    13681 tccggtagct cttcgagccc catcacctgt ttagtgttgg caatgaggaa cggcagcgcg
    13741 tcataaacgc ccggcgcatc ttcattgggt aaacccgctt tcatggaacg gtaagtgcct
    13801 acgccaacga agaccgcgtc gtattgttcc aaaagcgaat ccaaagagac atctttaccc
    13861 acttcacaat tgagttcgaa gtgaatcccc atcgcgctga agatttcccg acggcgtgcc
    13921 agcagggatt tatccagttt gaaagaagga atgccgaaag tgagcaagcc accgatttct
    13981 ggatggcgat cgtacaccgt cacccccacg ccattgcggg tcagaacatc cgcacaggcc
    14041 agccctgccg gacctgcacc gataatcgcc acccgcttgt ccactttggt gacatggctt
    14101 aagtcaggac gccaaccttt cgccaacgcc tgatctgaaa tgtagcgttc aatgttgccg
    14161 atagttaccg cgccgtgctc atcgcgaata gtacaggcac cttcacacaa acggtcttgc
    14221 ggacaaacgc gtccggtaat ttccggcagg gtgttggtct ggtgagaaag ctcgacggcg
    14281 gcgtcgatgt ttccggcttt caccagttca atccactgcg gtatatggtt atgcagcggg
    14341 caggtccatt cacaaacgct gtgctcgccg cacttaaggc agcgcgaggc ttcccgttgt
    14401 gcctggtcgg cgcgaaatgg cagataaatt tcatcaaaac cggttttgcg cgcttcaatc
    14461 gccagtttat ccggctcgcc acgcgcgggc gttgcctgca tttgttcgac tttactcatt
    14521 accggcattt cttgcgccgc ggtactggca tgccacggtt gatgttcctg acgcgcggtg
    14581 cgcaagcggc gggatttcgc catgccggag agtgcgacgt cagtgaccag ttgcagcgcg
    14641 tccgccgggc aattctcaac acaggcagga ccgttttcgc gccccgcaca aaggtcgcat
    14701 ttatgcgccg tggcttttac ttttcctgcc gcgacgggtg tcaggacgat tagcatcgta
    14761 ccaaaaggac aggccaccac gcaggattta cagccaatac acttttgctg attgacctga
    14821 atgctgtcat caacgtggct gattgcgcca ttagggcagc tacgggcgca gggcgcatct
    14881 tcacaatggt gacaggtcac tgcactacgt tgctgttgat gtttgataac cgtaattcgg
    14941 ggatgaaaat ggtgttggct caggacatgt tgctcatcat tgtgagccat gacacaggcg
    15001 atttcacaag catgacaacc cagacattgc tgactgttgg ccataataaa acgattcata
    15061 acgaccttct tttttggttg taaaaacctt attctttata tgagtgttgt tattacccga
    15121 cttacagggg aatcggcaat gttcatgtgc ccagaataag taactatttc gctataaact
    15181 gtggcagatc aaataatccc atcactgact aaattgtgtt tcagctaact ggaacattaa
    15241 tgattttttg tggagaagac gcgtgtgatt gttaaacgac ccgtctcggc cagtctggcc
    15301 cgggcctttt tttacattgt gctgctgtcg attctttcca cgggtatcgc tctgctaact
    15361 ctggcgagca gtttgcgcga cgctgaggct atcaatattg ccggatcgct acgtatgcag
    15421 agttaccgcc tgggctacga cttgcaaagt ggcagtccac aactcaatgc acatcgccag
    15481 ttgtttcagc aggcactgca ttcaccggta ttaaccaatc tcaacgtctg gtatgtgcca
    15541 gaagcagtaa aaactcgcta tgcgcatctg aatgccaact ggctggagat gaataatcgg
    15601 ctcagcaagg gcgatttgcc gtggtatcag gccaatatta ataattatgt taatcagata
    15661 gacctgttcg tgctggcttt acagcactac gctgaacgca aaatgctgct ggtggtggcg
    15721 atttccctgg ctggcggtat cggtattttc acgctggtct tttttacttt gcgccgtata
    15781 cgccatcagg tggttgcccc gctgaatcag ctggttaccg ccagtcagcg tattgaacac
    15841 gggcagttcg actcgccgcc gctggatacc aacctgccga atgagcttgg cctgcttgca
    15901 aaaaccttta accagatgtc gagcgagctg cataaattgt acctttcgct ggaggcgtca
    15961 gtagaagaaa agacccgcga tctccacgag gccaagcgtc gtctggaggt gttgtatcaa
    16021 tgttcgcagg cgctgaacac cagccagatt gatgtgcatt gtttccgcca tattttgcag
    16081 attgttcgcg acaatgaagc ggctgagtat ctggagttaa atgtcggtga aaactggcgg
    16141 attagcgaag ggcaaccaaa cccggaattg ccgatgcaga ttttaccggt gacaatgcaa
    16201 gagacggttt acggtgaact gcactggcaa aatagtcacg tttcatcatc agaaccgctg
    16261 cttaacagcg tttcatcgat gctgggacgc ggtttgtact ttaatcaggc gcagaagcat
    16321 tttcagcagt tattgttgat ggaagaacgt gcgaccatcg cccgcgaatt gcacgactcg
    16381 ctggctcagg tgctttctta cttacgtatc cagttgacgt tactgaagcg ttcgataccg
    16441 gaagataacg ccaccgcaca aagtatcatg gccgattttt cccaggcgtt gaatgatgct
    16501 tatcggcagt tacgcgagct gttgactact ttccgcctga cgctgcagca ggcggatctc
    16561 ccctccgcgt tgagggaaat gctggatacg ttacaaaatc aaaccagcgc caaactgacc
    16621 ctcgactgcc gtctgccaac cctggcgctg gatgcgcaaa tgcaggtgca tttgttgcaa
    16681 attattcgcg aagcggtgct gaatgcgatg aagcacgcca acgccagcga aatcgccgtc
    16741 agttgcgtca ccgcgccgga cggcaatcac acggtttata tccgtgataa cgggattggt
    16801 atcggtgaac cgaaagaacc cgaaggtcat tatggtctga atatcatgcg cgaacgcgcg
    16861 gaacggctgg gtgggacgct gactttttca caaccttccg gcggcggcac gttagtgagt
    16921 attagctttc gctctgcgga gggtgaggaa agtcagttga tgtaaagcct cctactgacc
    16981 aaagaatact tgcacttaag gttcagtata aaagggcatg ataatttaca ttaactcctt
    17041 tttttctcca cgattggctc gtaccttgcc gctacagtga agcaagtcaa gcctacaacg
    17101 atacgcagaa acacgaggtc ctcttttaat ggcgaatttc tttattgatc gccccatttt
    17161 tgcctgggtg ctggcaatcc tgttgtgtct gacagggacc ctggcgattt tttcattgcc
    17221 cgttgaacaa taccccgatc tcgcgccacc gaatgtgcga gtgaccgcta actatcccgg
    17281 cgcatcggcc cagacgctgg aaaacaccgt gacccaggtt atcgagcaaa atatgaccgg
    17341 cctcgataat ctcatgtata tgtcatctca gagcagtggc accggtcagg catctgtcac
    17401 tttaagtttt aaagcaggca ccgatccgga cgaagccgtg cagcaagtac aaaaccagct
    17461 gcaatcagcc atgcgaaagt taccgcaggc ggtgcaaaat cagggcgtga cggtgcgtaa
    17521 aaccggcgat accaatattc tgaccattgc tttcgtctct accgatggtt cgatggataa
    17581 acaggatatt gccgattatg ttgccagtaa tattcaggac ccgttaagcc gcgtgaatgg
    17641 cgtcggggat atcgatgcct atggttcgca atattccatg cgtatctggc tggacccggc
    17701 gaaactcaac agtttccaga tgacggctaa agatgtcact gatgccattg agtcacagaa
    17761 cgcgcagatt gcggttgggc aacttggtgg tacaccttcc gtcgataagc aggcgctcaa
    17821 cgccaccatt aacgcccagt cactgctgca aacaccagaa cagttccgcg atatcacctt
    17881 gagggtgaat caagacggct cagaggtaag gctgggcgat gtcgccaccg tcgaaatggg
    17941 ggcggagaaa tacgattatc ttagccgctt caatggtaag ccagcctccg ggctgggggt
    18001 aaaactggcc tccggcgcga acgaaatggc gacagcggag ctggtgctca atcgtctcga
    18061 cgatctggca cagtatttcc cgcacggact ggaatacaag gtggcgtatg aaaccacctc
    18121 gtttgttaaa gcctccattg aagacgtggt gaaaacgctg ctggaagcta tcgccctggt
    18181 tttcctcgtt atgtatctgt tcctgcaaaa cttccgcgcc acgctgatac ccactatcgc
    18241 cgtgccggtg gtgttgatgg gaaccttctc cgtactttac gccttcggtt acagcgtcaa
    18301 caccttaacc atgttcgcga tggtactggc tatcggtctg ctggtggatg acgccatcgt
    18361 ggtggtggaa aacgtcgaac gtattatgag tgaggaagga ctcactcctc gcgaagccac
    18421 acgtaaatcg atggggcaga tccagggggc actggtcggg attgcgatgg ttctttcggc
    18481 ggtatttgta ccaatggcct tcttcggcgg caccaccggt gccatctatc gccagttctc
    18541 tattaccatt gttgcggcga tggtgctgtc agtactggta gcgatgatcc tcactccggc
    18601 tctgtgtgcc acactactta agccactgaa aaaaggtgag catcacgggc aaaaaggctt
    18661 ttttgcctgg tttaaccaga tgtttaaccg caacgccgaa cgctacgaaa aaggggtggc
    18721 gaaaattctc caccgtagcc tgcgctggat tgtgatttat gtcctgctgc ttggcggcat
    18781 ggtgttcctg ttcctgcgtt tgccgacgtc gttcttaccg ctggaagacc gtggcatgtt
    18841 tactacctcg gtacaattgc ccagcggttc aacccaacaa cagaccctga aagtcgttga
    18901 gcaaatcgag aaatattact tcacccatga aaaagacaac atcatgtcgg tgtttgccac
    18961 tgttggttct ggccccgggg gtaacgggca aaacgtggcg cgaatgttta tccgcctgaa
    19021 agactggagc gaacgcgaca gtaagaccgg cacctcattt gccattatcg agcgtgcaac
    19081 gaaggcgttt aacaagatta aagaagctcg cgttatcgcc agcagcccgc cagcaattag
    19141 cggtcttggt agttctgcag gttttgatat ggagttgcag gaccacgctg gagcaggtca
    19201 cgatgcgctg atggcagcac gtaaccagtt gctggcgctg gcggcggaaa acccggagct
    19261 aacccgtgtg cgccataacg gcctcgatga cagtccgcag ttgcagattg atatcgacca
    19321 gcgtaaagct caggcgctgg gcgttgctat cgacgatatt aacgacacac tgcaaaccgc
    19381 ctggggttcg agctatgtga atgactttat ggatcgcggt cgcgtgaaga aagtctatgt
    19441 gcaggcagct gcgccgtatc gcatgctgcc agatgacatt aatctctggt atgtccgaaa
    19501 taaagatggc ggcatggtgc ccttctctgc tttcgcgacc tcacgctggg agacaggctc
    19561 gccgcgtctg gaacgctata acggttattc tgcggttgag attgttgggg aagccgcacc
    19621 gggggtcagt accggtacgg cgatggatat tatggaatcg ttagtgaagc agttgccaaa
    19681 cggctttggt ctggagtgga cggcgatgtc gtatcaggag cggctttccg gcgcgcaggc
    19741 tccggcgctg tacgccattt ccttgctggt agtattcctg tgtctggcgg cattgtatga
    19801 aagctggtcg gtaccgttct cggtaatgct ggtcgtgccg ctgggggtta tcggcgcgct
    19861 gctggcaacc tggatgcgcg ggctggaaaa cgacgtttac ttccaggtgg gcctgttaac
    19921 ggtcattggt ttatcggcga aaaacgccat cctgatcgtc gagtttgcta acgagatgaa
    19981 ccaaaaaggc cacgacctgt ttgaagcgac gctccacgcc tgccgtcagc gtttacgccc
    20041 gattctgatg acctcgctgg catttatctt cggcgtattg ccaatggcaa ccagcacggg
    20101 tgccggttcc ggtggtcagc atgcggtggg tactggcgta atgggcggga tgatttcggc
    20161 cactattctg gctatttact tcgtgccgct gttctttgta ctggtgcgcc gccgcttccc
    20221 gctgaagccg cgcccggaat aagcaataaa aaaggcgaca tgctaatgtg tcgccttttt
    20281 caactttccg attaagaacc tgctcagcgg gttcttgctg tttgtacttt gtctcaggaa
    20341 ttacttacga agcataactt caataaagtc tttccagttc cccagttcac gttcaatcat
    20401 aacaacctct cttataatta tgggtattct acggaaacaa tataccgtgg tgaagctaat
    20461 ttactcgatt gctgcgatga ctacctccgg gggacaaacc ttatgtaaat actatggtcc
    20521 tgcggtgata atttgtatgt gatacacagc aacatttcga gatattcata cggcatctaa
//
LOCUS       DAJOSY010000080        20141 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJOSY010000080 DAJOSY010000000
VERSION     DAJOSY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.080"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            521..1510
                     /gene="araF"
                     /locus_tag="OHN27_003065"
     CDS             521..1510
                     /gene="araF"
                     /locus_tag="OHN27_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCQ2287459.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1580..3094
                     /gene="araG"
                     /locus_tag="OHN27_003066"
     CDS             1580..3094
                     /gene="araG"
                     /locus_tag="OHN27_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCQ2287460.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3109..4095
                     /gene="araH"
                     /locus_tag="OHN27_003067"
     CDS             3109..4095
                     /gene="araH"
                     /locus_tag="OHN27_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCQ2287461.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4262..5062
                     /gene="otsB"
                     /locus_tag="OHN27_003068"
     CDS             4262..5062
                     /gene="otsB"
                     /locus_tag="OHN27_003068"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCQ2287462.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAAVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5037..6461
                     /gene="otsA"
                     /locus_tag="OHN27_003069"
     CDS             5037..6461
                     /gene="otsA"
                     /locus_tag="OHN27_003069"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCQ2287463.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6468..6896)
                     /gene="uspC"
                     /locus_tag="OHN27_003070"
     CDS             complement(6468..6896)
                     /gene="uspC"
                     /locus_tag="OHN27_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCQ2287464.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            7675..8025
                     /gene="flhD"
                     /locus_tag="OHN27_003071"
     CDS             7675..8025
                     /gene="flhD"
                     /locus_tag="OHN27_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCQ2287465.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            8028..8606
                     /gene="flhC"
                     /locus_tag="OHN27_003072"
     CDS             8028..8606
                     /gene="flhC"
                     /locus_tag="OHN27_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCQ2287466.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            8733..9620
                     /gene="motA"
                     /locus_tag="OHN27_003073"
     CDS             8733..9620
                     /gene="motA"
                     /locus_tag="OHN27_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCQ2287467.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            9617..10543
                     /gene="motB"
                     /locus_tag="OHN27_003074"
     CDS             9617..10543
                     /gene="motB"
                     /locus_tag="OHN27_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCQ2287468.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            10548..12512
                     /gene="cheA"
                     /locus_tag="OHN27_003075"
     CDS             10548..12512
                     /gene="cheA"
                     /locus_tag="OHN27_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCQ2287469.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            12533..13036
                     /gene="cheW"
                     /locus_tag="OHN27_003076"
                     /pseudo
     CDS             12533..13036
                     /gene="cheW"
                     /locus_tag="OHN27_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
     gene            13181..15208
                     /gene="flhA"
                     /locus_tag="OHN27_003077"
     CDS             13181..15208
                     /gene="flhA"
                     /locus_tag="OHN27_003077"
                     /inference="COORDINATES: protein motif:HMM:NF014281.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HCQ2287470.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNKQMAIDADLNAGLIG
                     EDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVINVVGGLLVGVLQHGMSM
                     GHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQDVGEQMVNQLFSNPSVML
                     LSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKAPAEPKPVKMAENNTVVE
                     ATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRKKFAQEMGFLPPVVHIRD
                     NMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLPGEATVDPAFGLNAIWIE
                     SALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQEAQQLLDRVAQEMPKLTE
                     DLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAPIQSDPHELTAVVRVALG
                     RAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGLADRLLAQTQEALSRQEM
                     LGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRHIRMTAIIGGK"
     gene            15208..15600
                     /gene="flhe"
                     /locus_tag="OHN27_003078"
     CDS             15208..15600
                     /gene="flhe"
                     /locus_tag="OHN27_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCQ2287471.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(15721..16209)
                     /locus_tag="OHN27_003079"
                     /pseudo
     CDS             complement(15721..16209)
                     /locus_tag="OHN27_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(16386..18119)
                     /gene="argS"
                     /locus_tag="OHN27_003080"
     CDS             complement(16386..18119)
                     /gene="argS"
                     /locus_tag="OHN27_003080"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCQ2287472.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            18335..18901
                     /locus_tag="OHN27_003081"
     CDS             18335..18901
                     /locus_tag="OHN27_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCQ2287473.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            18915..19661
                     /gene="cutC"
                     /locus_tag="OHN27_003082"
     CDS             18915..19661
                     /gene="cutC"
                     /locus_tag="OHN27_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCQ2287474.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
BASE COUNT         5044 a         4748 c         5264 g         5085 t
ORIGIN      
        1 tgaattcatg atgaagtgat gattttataa aacgtttttt catttttgcg agatggctct
       61 cattatacgt gttctgttaa ttaatgagca cagtgataat taatgacgaa atgaatgacg
      121 tgcattttcc acatctttga gttgcggtta ttacaccatt tcaaaaactc aacgccaggt
      181 aatgcggcct attgactggt taaaaagaag acatcccgca tgggtaccaa agacaacaag
      241 gatttccagg ctaatcttat ggattaatct gctgtgcatt cgacaatttg tctgacaaat
      301 tggctttccc ttatgtcttt tcccgctaaa tttatgcacg ttctcactgt aattctgcga
      361 tgtgatattg ctctcctatg gagaattaat ttctcgctaa aactatgtca acacagtcac
      421 ttatctttta gttaaaaggt aatgctttgt tttccgatta atttaacgaa tgtcattcgt
      481 ttttgcccta cacaaaacga cactaaagct ggagagaacc atgcacaaat ttactaaagc
      541 cctggcagcc attggtctgg cagccgttat gtcacaatcc gctatggcgg agaacctgaa
      601 gctcggtttt ctggtgaagc aaccggaaga gccgtggttc cagaccgaat ggaagtttgc
      661 cgataaagcc gggaaggatt tagggtttga ggttattaag attgccgtgc cggatggcga
      721 aaaaacattg aacgcgatcg acagcctggc tgccagtggc gcaaaaggtt tcgttatttg
      781 tactccggac cccaaactcg gctctgccat cgtcgcgaaa gcgcgtggct acgatatgaa
      841 agtcattgcc gtggatgacc agtttgttaa cgccaaaggt aagccaatgg ataccgttcc
      901 gctggtgatg atggcggcga ctaaaattgg cgaacgtcag ggccaggaac tgtataaaga
      961 gatgcagaaa cgtggctggg atgtcaaaga aagcgcggtg atggcgatta ccgccaacga
     1021 actggatacc gcccgccgcc gtactacggg atctatggat gcgctgaaag cggccggatt
     1081 cccggaaaaa caaatttatc aggtacctac caaatctaac gacatcccgg gggcatttga
     1141 cgctgccaac tcaatgctgg ttcaacatcc ggaagttaaa cattggctga tcgtcggtat
     1201 gaacgacagc accgtgctgg gcggcgtacg cgcgacggaa ggtcagggct ttaaagcggc
     1261 cgatatcatc ggcattggca ttaacggtgt ggatgcggtg agcgaactgt ctaaagcaca
     1321 ggcaaccggc ttctacggtt ccctgctgcc aagcccggac gtacatggct ataaatccag
     1381 cgaaatgctt tacaactggg tagcaaaaga cgttgaaccg ccaaaattta ccgaagttac
     1441 cgacgtggta ctgatcacgc gtgacaactt taaagaagaa ctggagaaaa aaggtttagg
     1501 cggtaagtaa tttgccggaa aaattcccct ctgcatgatg cagagggggt gtgaacgacc
     1561 agtgattcac ggagacgtta tgcaacagtc taccccgtat ctctcatttc gcggcatcgg
     1621 taaaacgttt cccggcgtta aggcgctgac ggatattagt tttgactgct atgccggtca
     1681 ggttcatgcg ttgatgggtg aaaatggcgc aggaaaatca actctcttaa aaatcctcag
     1741 cggcaactat gcgccaacca cgggttctgt agtgattaat gggcaggaaa tgtccttttc
     1801 cgacacgacc gcagcactta acgcgggcgt ggcgattatt taccaggaac tgcatctcgt
     1861 gccggaaatg accgtcgcgg aaaacatcta tctcggccag ctgccgcata aaggcggcat
     1921 tgtgaatcgc tcattgctga attatgaggc gggtttacaa cttaaacatc ttggtatgga
     1981 tattgacccg gacacgccgc tgaaatatct ctccattggt cagtggcaga tggttgaaat
     2041 cgccaaagcg ctggcgcgta acgccaaaat tatcgccttt gatgagccaa ccagctccct
     2101 ctctgcccgt gaaatcgaca atcttttccg cgttattcgt gaactgcgaa aagaggggcg
     2161 ggtgatctta tacgtttctc accgtatgga agaaatattt gccctcagcg atgccattac
     2221 tgtctttaaa gatggacgtt atgtcaaaac ctttaccgat atgcagcagg ttgaccacga
     2281 cgcgctggtg caggcgatgg tcgggcgcga cattggcgat atctacggct ggcaaccgcg
     2341 tagttatggc gaggagcgcc tacgtcttga tgctgtgaaa gcaccaggcg tgcgtacgcc
     2401 aataagtctg gcggttcgca gtggtgaaat tgttgggctg tttggtctgg taggggcggg
     2461 gcgtagcgaa ttaatgaaag gcatgtttgg cgggacgcaa atcaccgccg gtcaggttta
     2521 tatcgaccaa cagccgatcg atattcgtaa accgagccac gccattgccg caggcatgat
     2581 gctctgcccg gaagatcgca aagcggaagg cattattccc gtgcactccg ttcgcgacaa
     2641 tatcaacatc agtgccagac gtaaacatgt gctcggcggt tgtgtaatca acaacggttg
     2701 ggaagaaaac aatgccgatc accacattcg ttcgctcaac atcaaaacgc cgggcgcgga
     2761 gcaactgatc atgaatctct caggcggaaa tcagcaaaaa gccattctgg gccgctggtt
     2821 atcggaagag atgaaggtca ttttgctgga tgaacctacg cgcggcattg atgttggcgc
     2881 taagcacgaa atatataacg taatttatgc gctggcggcg cagggcgtgg cggtgctgtt
     2941 tgcctccagc gacttacctg aagtcctcgg cgttgccgac cggattgtgg tgatgcggga
     3001 aggtgaaatc gccggtgaat tgttacacga gcaggcagat gagcgtcagg cactgagcct
     3061 tgcgatgcct aaagtcagcc aggctgttgc ctgagtaagg agagtatgat gtcttctgtt
     3121 tctacatcgg ggtctggcgc acctaagtcg tcattcagct tcgggcgtat ctgggatcag
     3181 tacggcatgc tggtggtgtt tgcggtgctc tttatcgcct gtgccatttt tgtcccaaat
     3241 tttgccacct tcattaatat gaaagggttg ggcctggcaa tttccatgtc ggggatggtg
     3301 gcttgtggca tgttgttctg cctcgcttcc ggtgactttg acctttctgt cgcctccgta
     3361 attgcctgtg cgggtgtcac cacggcggtg gttattaacc tgactgaaag cctgtggatt
     3421 ggcgtggcag cggggttgtt gctgggcgtt ctctgtggcc tggtcaatgg ctttgttatc
     3481 gccaaactga aaataaatgc tctgatcacg acattggcaa cgatgcagat tgttcgaggt
     3541 ctggcgtaca tcatttcaga cggtaaagcg gtcggtatcg aagatgaaag cttctttgcc
     3601 cttggttacg ccaactggtt cggtctgcct gcgccaatct ggctcaccgt cgcgtgtctg
     3661 attatctttg gtttgctgct gaataaaacc acctttggtc gtaacaccct ggcgattggc
     3721 gggaacgaag aggccgcgcg tctggcgggt gtaccggttg ttcgcaccaa aattattatc
     3781 tttgttctct caggcctggt atcagcgata gccggaatta ttctggcttc acgtatgacc
     3841 agtgggcagc caatgacgtc gattggttat gagctgattg ttatctccgc ctgcgtttta
     3901 ggtggcgttt ctctgaaagg tggcatcgga aaaatctcat atgtggtggc gggtatctta
     3961 attttaggca ccgtggaaaa cgccatgaac ctgcttaata tttctccttt cgcgcagtac
     4021 gtggttcgcg gcttaatcct gctggcagcg gtgatcttcg accgttacaa gcaaaaagcg
     4081 aaacgcactg tctgatgctt ttttctgcaa caatttagcg ttttttccca ccatagccaa
     4141 ccgccataac ggttggctgt tcttcgttgc aaatggcgac ccccgtcaca ctgtctatac
     4201 ttacatgtct gtaaagcgcg ttctgcgcaa cacaataaga aaagagaagg aggagaaccg
     4261 ggtgacagaa ccgttaaccg aaacccctga actatccgcg aaatatgcct ggttttttga
     4321 tctcgatgga acgctggcgg aaatcaaacc gcatcccgat caggtcgtcg tgcctgacaa
     4381 cattctgcaa ggactacagc tactggcaac cgcaagtgat ggtgcattgg cattgatatc
     4441 agggcgctca atggtggagc ttgacgcact ggcaaaacct tatcgcttcc cgttagcggg
     4501 cgtgcatggg gcggagcgcc gtgacatcaa tggtaaaaca catatcgttc atctgccgga
     4561 tgcgattgcg cgtgatatta gcgtgcaact gcatacggtc atcgcccagt atcccggtgc
     4621 ggagctggag acgaaaggga tggcctttgc gctgcattat cgtcaggctc cgcagcatga
     4681 agacgcatta atgacattag cgcaacgtat tactcagatc tggccacaaa tggcgttaca
     4741 gcagggaaag tgtgttgtcg agatcaaacc gagaggtacc agtaaaggtg aggcgattgc
     4801 agcttttatg caggaagctc cctttatcgg gcgaacgccc gtatttctgg gcgatgattt
     4861 aaccgatgaa tctggcttcg cagccgttaa ccggctgggc ggaatgtcag taaaaattgg
     4921 cacaggtgca actcaggcat catggcgact ggcgggtgtg ccggatgtct ggagctggct
     4981 tgaaatgata accaccgcat tacaacaaaa aagagaaaat aacaggagtg atgactatga
     5041 gtcgtttagt cgtagtatct aaccggattg caccaccaga cgagcacgcc gccagtgccg
     5101 gtggccttgc cgttggcata ctgggggcac tgaaagccgc aggcggactg tggtttggct
     5161 ggagtggcga aacagggaat gaggatcagc cgctaaaaaa ggtgaaaaaa ggtaacatta
     5221 cgtgggcctc ttttaacctc agcgaacagg accttgacga atactacaac caattctcca
     5281 atgccgttct ctggcccgct tttcattatc ggctcgatct ggtgcaattt cagcgtcctg
     5341 cctgggacgg ctatctacgc gtaaatgcgt tgctggcaga taaattactg ccgctgttgc
     5401 aagacgatga cattatctgg atccacgatt atcacctgtt gccatttgcg catgaattac
     5461 gcaaacgggg agtgaataat cgcattggtt tctttctgca tattcctttc ccgacaccgg
     5521 aaatcttcaa cgcgctgccg acatatgaca ccttgcttga acagctttgt gattatgatt
     5581 tgctgggttt ccagacagaa aacgatcgtc tggcgttcct ggattgtctt tctaacctga
     5641 cccgcgtcac gacacgtagc gcaaaaagcc atacagcctg gggcaaagca tttcgaacag
     5701 aagtctaccc gatcggcatt gaaccgaaag aaatagccaa acaggctgcc gggccactgc
     5761 cgccaaaact ggcgcaactt aaagcggaac tgaaaaacgt acaaaatatc ttttctgtcg
     5821 aacggctgga ttattccaaa ggtttgccag agcgttttct cgcctatgaa gcgttgctgg
     5881 aaaaatatcc gcagcatcat ggtaaaattc gttataccca gattgcacca acgtcgcgtg
     5941 gtgatgtgca agcctatcag gatattcgtc atcagctcga aaatgaagct ggacgaatta
     6001 atggtaaata cgggcaatta ggctggacgc cgctttatta tttgaatcag cattttgacc
     6061 gtaaattact gatgaaaata ttccgctact ctgacgtggg cttagtgacg ccactgcgtg
     6121 acgggatgaa cttggtagca aaagagtatg ttgctgctca ggacccagcc aacccgggcg
     6181 ttcttgttct ttcgcaattt gcgggagcgg caaacgagtt aacgtcggcg ttaattgtta
     6241 acccctacga tcgtgacgaa gttgcagctg cgctggatcg tgcattgact atgtcgctgg
     6301 cggaacgtat ttcccgtcat gcagaaatgc tggacgttat cgtgaaaaac gatattaacc
     6361 actggcagga gtgcttcatt agcgacctaa agcagatagt tccgcgaagc gcggaaagcc
     6421 agcagcgcga taaagttgct acctttccaa agcttgcgta ggagcggtta atcacccgta
     6481 agtggaacta acagcacatc gacctcactt gaggcaatca ctcttttcgc cgagcaggat
     6541 gctcgcgaaa agaaactgtg attgtgatta ccgcaaatca ccaaatcgaa atgatgtttg
     6601 tgacatactt ccagaatatg ttcgcttaac tcaccgtagg caataaacgt cttgtcaacg
     6661 ggataccccg catcctgaat taacttatca agaaagcttt gcgtttcttc ttgcatcacg
     6721 ctgcgtaaat cttcaagcat cggcgcggct aattgattgt acatttccgg gtcggaagcg
     6781 agagtaatta aactgatgtg tccctttact ggcctggcga tagataccgc ttttgccagc
     6841 agttgctgac tttccggtgt aaccgcaaca gcgacaagaa tattgctata gctcataacc
     6901 tgttccttat tctgtgaact tcaggtgaca ttaaagcttt actcctaatg gagaaacgac
     6961 gcaatcccaa ctcggtcaaa caggtaaata ttagtctgat tattagtaaa gacaattaat
     7021 caatcactcc cggtgatcat ataatttcaa atgtaattat aagaaaatag tgtactgatt
     7081 ctccgcatta aacaagtggt taatatatgt aaaacatttc attaacatta tgttgattgt
     7141 tgcctttctt tgtatttaat tagtttgttg tgcggtaagt gtctgtttaa aaatagctta
     7201 tctggtattg catgaaagtg attatttata gcagatgatt atttacggtg agttatttta
     7261 actgtgcgca acatcccatt tcgattattc ctgtttcatt tttgcttgct agcgtagcga
     7321 aaaacttttt aacagattga aatacaccca aaacaaaagt atgacttata catttatgtt
     7381 aagtaattga gtgtcttgtg tgatctgcat cacacattat tgaaaatcgc agcccccctc
     7441 cgttgtatgt gcgtgtagtg acgagtacag ttgcgtcgat ttaggaaaaa tcttagataa
     7501 gtgtaaagac ccccttctat ttgtaaggac ttattaaacc aaaaaggtgg ctctgcttat
     7561 tgcagcttat cgcaactatt ctaatgctaa ttatttttac cggggcttcc cggcgacatc
     7621 acagggtgcg gtgaaaccgc ataaaaataa agttggttat tctgggtggg aataatgcat
     7681 acctccgagt tgctgaaaca catttatgac atcaacttgt catatttact acttgcacag
     7741 cgtctgattg ttcaggacaa agcgtccgct atgtttcgtc tcggcataaa tgaagaaatg
     7801 gcgacaacat tagcggcact gactcttccg caaatggtta agctggcaga aaccaatcag
     7861 ctggtttgtc acttccgttt tgacagccac cagacgatta ctcagttgac gcaagattcc
     7921 cgcgttgacg atctccagca aattcatacc ggcatcatgc tctcaacacg cttgctgaat
     7981 gatgttaatc agcctgaaga agcgctgcgc aagaaaaggg cctgatcatg agtgaaaaaa
     8041 gcattgttca ggaagcgcgg gatattcagc tggcaatgga attgatcacc ctgggcgctc
     8101 gtttgcagat gctggaaagc gaaacacagt taagtcgcgg acgcctgata aaactttata
     8161 aagaactgcg cggaagccca ccgccgaaag gcatgctgcc attctcaacc gactggttta
     8221 tgacctggga acaaaacgtt catgcttcga tgttctgtaa tgcatggcag tttttactga
     8281 aaaccggttt gtgtaatggc gtcgatgcgg tgatcaaagc ctaccgttta taccttgaac
     8341 agtgcccaca agcagaagaa ggaccactgc tggcattaac tcgtgcctgg acattggtgc
     8401 ggtttgttga aagtggatta ctgcaacttt ccagctgcaa ctgctgcggc ggcaatttta
     8461 ttacccacgc tcaccagcct gttggcagct ttgcctgcag cttatgtcaa ccgccatcca
     8521 gggcagtaaa aagacgtaaa ctttcccaga atcctgccga tattatccca caactgctgg
     8581 atgaacagag agtacaggct gtttaactga tacggtgagg cgcaacattc cagcagcggt
     8641 aacgacgtac cgctgctttt ttttgcccca atcgcgcgtt aacgcctgac gactgaacat
     8701 cctgtcatgg tcaacagtgg aaggatgatg tcgtgcttat cttattaggt tacctggttg
     8761 ttctcggtac agttttcggc ggttatttga tgaccggtgg aagccttgga gcactctatc
     8821 aacccgctga actggtgatt attgccggtg cagggattgg gtcgtttatc gtcggcaata
     8881 atggcaaagc gattaaaggc acgctgaagg cgctgccgtt gctgtttcgt cgctccaaat
     8941 acaccaaagc aatgtatatg gatctgctgg ctctgcttta tcggttgatg gcaaaatcgc
     9001 ggcagatggg gatgttttcg ctggaacgtg atattgaaaa tccccgtgag agcgagatct
     9061 tcgccagcta cccacgcatc ctcgcggata gcgtcatgct tgattttatc gtcgattatc
     9121 tgcgcctgat tatcagcggt cacatgaaca ccttcgaaat cgaagccctg atggatgaag
     9181 agattgagac gcacgaaagc gaggcagaag tcccggcgaa cagtctggcg ctggtcgggg
     9241 actcacttcc ggcgtttggt attgttgcgg ctgtaatggg ggtcgttcac gcgttaggtt
     9301 cagccgatcg tcctgccgcc gagctgggtg cgcttatcgc acatgcgatg gtggggactt
     9361 tcctcggcat tttattggct tacggattta tttccccatt agcgactgtt ttacgtcaga
     9421 aaagcgccga aaccagcaaa atgatgcagt gcgtcaaagt cactctgctt tctaatctga
     9481 acggttacgc accgcctatc gccgttgagt ttggtcgcaa aacgctctat tccagcgaac
     9541 gtccgtcgtt tattgaactg gaagagcatg tgcgtgcggt gaaaaatccg caacaacaga
     9601 cgacaaccga ggaagcatga agaatcaagc gcatccgatt attgtcgtca aacgacgcaa
     9661 agccaaaagc cacggggcag cacatggatc gtggaagatt gcttatgccg actttatgac
     9721 tgcgatgatg gccttttttc tggtgatgtg gctgatctcc atctccagcc caaaagagct
     9781 gattcagatt gcggagtact tccggactcc actggcgact gcggttacgg gcggcgatcg
     9841 catttctaat agtgaaagcc caattcccgg cggtggtgat gattacaccc aaagccaggg
     9901 ggaagtgaat aagcagccga acatcgaaga gctgaaaaaa cgcatggagc aaagtcgatt
     9961 gcggaaattg cggggtgatc tcgaccagtt gatagagtcc gatccgaaac tgcgggcgtt
    10021 acgtccccat ctcaaaatcg atctggtcca ggaaggtcta cgtattcaga tcatcgatag
    10081 ccagaatcgc ccgatgttta gaaccggcag tgccgatgtc gaaccctata tgcgcgacat
    10141 tctgcgcgcc attgcgcctg tactgaacgg tattcccaac cgtattagcc tttcaggtca
    10201 taccgatgat ttcccctacg ccagcggtga gaaaggatat agcaactggg agctttctgc
    10261 cgatcgggcc aatgcatccc gccgcgaact gatggtcgga gggttggata gcggcaaagt
    10321 gttacgtgtc gtcggcatgg cggcaacgat gcgcttaagc gatcgcggac ctgatgatgc
    10381 cgtcaaccgt cgcatcagcc tgctggtact gaacaaacaa gccgaacagg ccattttgca
    10441 tgaaaacgcc gaaagtcaga atgagccagt aagcgccctg gaaaaacctg aggttgcacc
    10501 acaggtcagt gttcccacaa tgccatcagc cgaaccgagg tgacagcgtg agcatggata
    10561 taagcgattt ttatcagaca ttttttgatg aagcggacga actgttggct gacatggagc
    10621 agcatttgct ggttttgcag ccggaagcgc cagatgccga acaattgaat gccatctttc
    10681 gggctgccca ctcgatcaaa ggaggggcag gaacttttgg cttcagcgtt ttgcaggaaa
    10741 ccacgcatct gatggaaaac ctgctcgatg aagccagacg aggtgagatg caactcaaca
    10801 ccgacattat caatctgttt ttggaaacga aggacatcat gcaagaacag ctcgacgctt
    10861 ataaacagtc gcaagagccg gatgccgcca gcttcgatta tatctgccag gccttgcgtc
    10921 aactggcatt agaagcgaaa ggcgaaacgc catccgcagt gacccgatta agtgtggttg
    10981 ccaaaagtga accgcaagat gagcagagtc gcagtcagtc gccgcgacga attatccttt
    11041 cgcgcctgaa ggccggggaa gtcgacctgc tggaagaaga actgggacat ctgacaacgt
    11101 taactgacgt ggtgaaaggg gcggattcgc tctcggcaat attaccgggc gacatcgccg
    11161 aagatgacat cacagcggta ctctgttttg tgattgaagc cgatcagatt acctttgaaa
    11221 cagtagaagt ctcgccaaaa atatccaccc caccagtgct taaactggca gccgaacaag
    11281 cgccaaccgg ccgcgtggag cgggaaaaaa cgacgcgcag caatgaatcc accagcatcc
    11341 gtgtagcggt agaaaaggtt gatcaattaa ttaacctcgt cggcgagctg gttatcaccc
    11401 agtccatgct tgcccagcgt tccagcgaac tggacccggt taatcatggt gatttgataa
    11461 ccagcatggg gcagttacaa cgtaacgccc gtgatttgca ggaatcagtg atgtcgattc
    11521 gcatgatgcc gatggaatat gtttttagtc gctatccccg gctggtgcgt gatctggcgg
    11581 gaaaactcgg caagcaggta gaactgacgc tggtgggcag ttctactgaa ctcgacaaaa
    11641 gcctgataga acgcattatc gacccgctga cccacctggt acgcaatagc ctcgatcacg
    11701 gtattgaact gccagaaaaa cggctcgccg caggtaaaaa cagcgtcgga aatttaattc
    11761 tgtctgccga acatcagggc ggcaacattt gcattgaagt gaccgacgat ggggcggggc
    11821 taaaccgtga gcgaattctg gcaaaagcgg cctcgcaagg tttgactgtc agcgaaaaca
    11881 tgagcgacga cgaagtcgcg atgctgatat ttgcacctgg cttctccacg gcagagcagg
    11941 tcaccgacgt ctccgggcgc ggcgtcggca tggacgtcgt taaacgtaat atccaggaga
    12001 tgggcggtca tgtcgaaatc cagtcgaagc agggtactgg cactacgatc cgcattttac
    12061 tgccgctgac gctggccatc ctcgacggca tgtccgtacg cgttgcggat gaagttttca
    12121 ttctgccgct gaatgctgtt atggaatcac tgcaaccccg tgaagccgat ctccatccac
    12181 tggccggcgg cgagcgggtg ctggaagtgc ggggtgaata tctgcccatc gtcgaactgt
    12241 ggaaagtgtt caacgtcgcg ggcgcgaaaa ccgaagccac ccagggaatt gtggtgatct
    12301 tacaaagtgg cggtcgccgc tacgccttgc tggtggatca attaattggt caacaccagg
    12361 ttgtggttaa aaaccttgaa agtaactatc gcaaagtccc cggcatttct gctgcgacca
    12421 ttcttggcga cggcagcgtg gcactgattg ttgatgtctc cgccttgcag gcgataaacc
    12481 gcgaacaacg tatggcgaac accgccgcct gaatgagtaa aaaggtaaca atatgaccgg
    12541 tatgacgaat gtaacaaagc tggccagcga gccgtcaggc caggaatttc tggtatttac
    12601 ccttggtgat gaagagtacg gtattgatat cctgaaagtg caggagatcc gtggctacga
    12661 tcaggtaaca cggattgcga acacgccagc gtttatcaaa ggcgtcacga atctgcgcgg
    12721 cgttattgtg ccgattgttg acttacgaat taagttcagc caggtggatg tggactataa
    12781 cgacaacacg gtagttatcg tcctgaatct cggacagcgg gtggtcggca tcgtggttga
    12841 cggcgtctca gacgtgcttt cattgacggc ggagcaaatt cgtccggcac cggaatttgc
    12901 cgtgacgctt tcaacagaat atctcacttg actgggcgca ctgggcgacc ggatgttgat
    12961 tctggtgaac atcgaaaaac tgctgaacag cgaagagatg gcgctgttag atagcgcggc
    13021 gtcagaagtg gcgtaattct cccgatttcc tcaattgaaa tgaacccgat gatctgcgca
    13081 tcgggttttt tatttcaatt tcgcggcggg tggcatcagc aataaagttt cccccctcct
    13141 tgccgataac gagatcaact tgttttcagg aaggtgcctt atgattaacc gtatccgcgt
    13201 agtcacgctg ttggtaatgg tgctgggggt attcgcactg ttacagctta tttccggcag
    13261 tctgtttttt tcttcccttc accatagcca gaagagcttt gtggtttcca atcaattacg
    13321 ggaacagcag ggcgagctga cgtcaacctg ggatttaatg ctgcaaacgc gcattaacct
    13381 gagtcgttca gcggtacgga tgatgatgga ttcctccaat caacaaagta acgccaaagt
    13441 tgaattgctc gatagcgcca ggaaaacatt ggcgcaggca gcgacgcatt ataaaaaatt
    13501 caaaagcatg gcaccgttac ctgaaatggt cgctaccagt cgtaatattg atgaaaaata
    13561 taaaaactat tacacagcgt taactgaact gattgattat ctagattatg gcaataagca
    13621 gatggcgatt gacgccgacc ttaacgccgg attgattggt gaagatgagg cgaaaaaacg
    13681 ccgctccgaa gtgactcagg aagccgattt ttacggctca atggacgggg caagtaagtt
    13741 tgttcgcggc gatgccatcg ccgggatcct catcatggtc attaacgttg tcggcgggtt
    13801 gctggtcggc gtgctgcaac atggcatgag catgggacac gcggcggaaa gttatacgct
    13861 attgaccatt ggcgacggtc tggtggcaca aattccggcg ctggtgattt ctaccgccgc
    13921 gggggtcatc gttacgcgtg tcagcaccga tcaggatgtt ggcgagcaga tggtgaatca
    13981 gcttttcagt aacccaagcg ttatgttgtt aagcgccgcc gtgctcggtt tactcggcct
    14041 ggtgcctgga atgccgaacc tggtattttt gctgttcact gccggattgc tcgggctggc
    14101 ctggtggata cgcggacgcg aacaaaaagc gcctgccgaa cccaaaccgg taaaaatggc
    14161 agagaataat accgttgtcg aagcgacgtg gaacgatgta caactggaag attctctggg
    14221 aatggaagtg ggttatcgac tgatcccgat ggtcgatttc cagcaggatg gtgagttgtt
    14281 gggccgtata cgcagtatcc gcaagaaatt tgcccaggag atgggatttc tgccgccagt
    14341 ggtgcacatt cgcgacaata tggatctgca acctgcccgc tatcgcattt tgatgaaagg
    14401 cgtggagatt ggcagtggtg atgcttatcc ggggcgctgg ctggcgatta accctggaac
    14461 cgctgccggg acgttacctg gtgaggcgac cgtcgatccg gcatttggcc tgaatgctat
    14521 ctggattgaa agtgcgctaa aagaacaggc gcagattcag gggtacacag tggttgaggc
    14581 cagcacggtg gtagcaacgc atcttaacca cctcattagc cagcatgccg cagagctgtt
    14641 tggtcgtcag gaggcgcaac agctgttgga tcgcgtcgcc caggagatgc caaagctgac
    14701 ggaagatctc gttcctggcg tcgtcacgct caccacactg cataaagtgc tgcaaaatct
    14761 cctcgatgaa aaagtaccga ttcgcgatat gcgcaccatt ctcgaaacgc tggcggaaca
    14821 tgcgcccatc caaagcgatc cacatgaatt aaccgccgtc gtgcgcgtgg cgttgggacg
    14881 ggcgattacc cagcagtggt ttcctggcaa agatgaagtc catgttattg gcctcgatac
    14941 accgctggaa cgtttgttac tacaggcgct gcagggcggg ggaggactgg agccagggct
    15001 ggcggatcgt ttactggcgc aaactcagga agcgctatcc cgtcaggaga tgctgggtgc
    15061 gccgccagta ttgttggtga accacgcgct gcgaccatta ttgtctcgct tcctgcgccg
    15121 cagcttgccg cagttagtgg tcctgtcgaa tctggaactg tctgataacc gacatatccg
    15181 catgacggcg ataattggcg gcaaataatg agaaccttat tagcaatatt attgtttccg
    15241 ctgctggtgc aagccgccgg ggaggggatg tggcaggcaa gtagtgtagg aattacgcta
    15301 aatcatcgcg gtgagtcgat gtcgtctgcg cctctttcta cgcgacaacc tgcttcagga
    15361 ttgatgacgc tggtagcgtg gcgttatcag cttatcggcc cgacaccttc aggactgcgg
    15421 gttcgcttgt gttcgcaatc tcgttgtgtc gaattagagg ggcagagcgg aaccaccgtg
    15481 gccttttccg gtatagcggc agcagaaccg ttgcgattta tctgggaagt gcctggcggt
    15541 gggcggttaa ttccgtcgct gaaagtacaa cgtaatgaag tgattgtgaa ttatcgctga
    15601 aggacaaaaa aaattacgcg ctcgcgagta tgtttaataa ctcagtgaaa aaggctggat
    15661 gttttgttgc aatcatttat caggaataga ttgcaacaaa acatgccaaa aatatgtgaa
    15721 ttatcctctt gtcaaagggc ttggataaaa atcttcagag ttacggtata gctgcaactg
    15781 gcgagttcgc gtattggtct gtatgatcat acatgagagc cattctgatt cagaataagg
    15841 gtcacttaaa tcctgaacgt cctcatttga aaggattaat tcacattctt tgtccctaaa
    15901 atccagccag gctcgctgtg attttttaag caagttaagt tattcggtat caggtttttt
    15961 tacacctgtg cgtggcactt gttctatata acgataagct tcaccatagg ctttatttaa
    16021 ttgcgcatcg acgagattgt attcttgtcg caggcatgat ttgatcacgt taacgtcagt
    16081 atcatcatct gaattttttt gccaacaatc tttgatggct gaaatgtcat ggtcgtttac
    16141 tataaccgta tcttctgcgt aagcctggca agagataatg cctaatgtta atacaagaaa
    16201 tttaaacact gcggtattcc ttaaattcaa ccgttatcgt ttccctgggg ccggatatta
    16261 cgcttctggc ctcttatcca acagtgagaa aacaatggca tgtccatttt cagaatacat
    16321 ttagatggca ataaaaaagc ctgatcaggt aaccctgatc aggctttact ctcacgaaaa
    16381 atcgattaca tacgctctac tgtctcaata cccagcgtat ccagacccag ctttagcgtc
    16441 ttcgctgtca gttgtgccag tttcagacgg ctgttacgca cttcttcgtt ttctgcgctg
    16501 aggatcgggc agtgctcgta gaagccagag aacagaccag ccagatcgta caggtaagca
    16561 cacatcacat gcggcgtgcc ttcacgggca accacggtga gggtttcttc aaactgcagc
    16621 aggcgagctg ccagttgcgc ttcacgatct tcacggatga ttaccggagc tgcagccagt
    16681 tgctcttcgt taatttctgc cttacggaac acggacaata cacgcgtgta agcatactgc
    16741 atgtatggcg cggtattacc ctcaaacgcc agcatgttgt cccagtcgaa gatgtagtcc
    16801 gtggtgcggt ttttggagag atccgcatat ttcaccgcac caataccaac cgcgttagcc
    16861 agtttttcca gctcgtcggc tggcatatcc gggttctttt ctgccaccag acggcgtgca
    16921 cgttccaggg cttcatccag cagatcggcc agtttcactg taccacccgc gcgggttttg
    16981 aacggtttgc cgtctttacc cagcatcatg ccgaacatgt ggtgttccag cggtacggat
    17041 tccggtacat agcctgcttt acggacgatc gcccatgcct gcatcaggtg ttgatgctga
    17101 cgggagtcga tgtaatacag cacgcgatcg gcatgcagtg tttcataacg atatttcgca
    17161 caggcgatat cagtggtggt gtagagatag ccgccatctt tcttctgaat gatcacgccc
    17221 atcggttcgc cttccttgtt tttaaactca tcaaggaata caacggtcgc cccttcgctt
    17281 tctactgcca gacctttggc tttgagatcc gccacaattc ctggcagcat tgggttgtag
    17341 aggctttcgc ccatcacgtc atcacgggtc agcgtcacgt tgagacgatc gtaagtgatc
    17401 tggttctgcg tcatggtgat gtcgaccagt ttgcgccaca tctcgcggaa atattcgtca
    17461 ccgctttgca gttttaccac gtagttacgt gcacgctcgg caaactcttc atcttcgtcg
    17521 taatgctttt tcgcatcgcg gtagaaacct tcaaggtcag ccagctccat ttcaccggcg
    17581 ttttcctgct gctgcttttc cagccatgca atcagcatac cgaactgagt gccccagtcg
    17641 ccgacgtggt ttgcgcgaat cactttgtga ccgaggaact ccagagtacg cactgctgcg
    17701 tcaccaataa tggtagagcg caggtgaccg acgtgcatct ctttcgccac gtttggcgca
    17761 gagtagtcaa ccacaatggt ctgtttttct ggcgtagcaa caccgagacg atcggacgcc
    17821 agcgcctgct gaacatgttc agccaggaat gccggatcaa ggaaaatgtt gataaagcct
    17881 ggaccggcga tctcaacttt gctggcgata ccgttaagat ccagatgagt cagcacctgc
    17941 tctgctaatt gtcgcggtgc catacccagt tttttagcaa ctgccatcat gccgttagcc
    18001 tgatagtcgc cgaactgaac ttttgctgac tgacgaacct gcggttcgca atccgcaggc
    18061 gcgcctgccg caatcatggc ctgacggact ttttctgaga gaagagcctg aatattcacc
    18121 ggaatacctt acattgatga agcgagcaaa aattgcccgc accagttaaa gaaattaggg
    18181 cgggagtata ctgcaaatgg ctgttttcgt cagcaacgtg cgcgtggcgt attgccgttt
    18241 tcactattgc atcagaagcg gcgtgaaatc agcggttggt tggcacaggg caacagggta
    18301 aattaacgct tttactgaaa gacgaagagt gattatggcg aactggcaat ctatcgacga
    18361 gctgcaggat attgcatcgg atttaccgcg atttacccac gcattagatg aactttcccg
    18421 ccgtctgggg ttgaatatca ctccgctgac tgccgatcac atttctttgc gttgccatca
    18481 aaacgccact gctgaacgct ggcgtcgcgg gtttgaacag tgtggcgagc ttttgtcaga
    18541 aaatatgatc aatggcagac cgatttgtct gtttaaattg catgaaccgg tacaggttgc
    18601 gcactggcag ttttctattg tggagctacc gtggcccggg gaaaaacgtt acccacacga
    18661 aggttgggaa catattgaaa tcgttctgcc gggtgatcca gaaacgctga atgcccgtgc
    18721 gctggcattg ctttctgatg aaggactcag cctaccgggg atctcagtga aaaccagttc
    18781 gccaaaaggt gagcatgagc gattaccgaa tccgacgctg gcggtcaccg atggcaaaac
    18841 aactattaag tttcatccgt ggtccattga agagatcgtt gccagcgagc agtcggcgta
    18901 attaaggagt aaaaatggca ttactggaaa tttgctgtta cagcatggaa tgtgcactaa
    18961 cggcgcagca aaacggcgca gacagagttg aattatgcgc agccccaaaa gaggggggct
    19021 taacgccgtc gttgggtgta ctgaaatccg tgcgccagcg ggtgacgatc cctgtgcatc
    19081 cgataattcg cccacgcggt ggtgattttt gctacagcga cggtgagttt gccgccattc
    19141 ttgaggatgt gcgcacggtc cgcgaattag gttttcctgg actggtgacg ggcgttctcg
    19201 atgttgacgg gaatgtcgat atgccacgaa tggaaaaaat aatggctgct gccggtccgc
    19261 tggcagtgac ttttcatcgc gcctttgata tgtgcgctaa ccctctaaat acactcaata
    19321 atcttgcgga attgggcatt gcccgggtgc tgacatcagg gcaaaaatca gacgcactgc
    19381 aaggtttatc aaaaattatg gaacttattg cccatcgtga tgctccaatc attatggccg
    19441 gagcaggagt ccgtgcagaa aacctgcacc acttcctcga tgccggagtg ctggaagtcc
    19501 atagctccgc gggagcgtgg caagcctcac cgatgcgtta tcgtaatcaa ggattgtcta
    19561 tgtcatcaga tgcacacgcg gacgagtatt cgcgttatgt cgtagacggg gcggcggttg
    19621 ctgaaatgaa aggaatcatt gaacgccatc aggccaaatg atttttaccg ttgcatcatg
    19681 tcgcccaata tgatgcttgc tcgtaccagg cccctgcaat ttcaacaggg gccttttttt
    19741 atccctgagc agtataaaaa acgaacgata accgtgatct gttgagcggg tgacagtgcg
    19801 catagcgttg tgctaaaaat attttttata ttcacattga ttatgggatt taaattgcta
    19861 aaactgataa atatatattc taaatagcaa ctgggttatt ccttagcaat taatgattac
    19921 attgtaataa atcatattct ttatcgatta tttcaggcag tgttgtgccg agttatgcaa
    19981 gcggttaatt cgttgtgtat ttaattatac aatgatttcg gtgttctgga atttatttag
    20041 aggaatctat gcgagggaaa aaacgcattg ggttattgtt tttgctgata gcggttgtgg
    20101 ttggtggcgg cgggttattg ctggcgcaaa aagccttaca t
//
LOCUS       DAJOSY010000081        19460 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJOSY010000081 DAJOSY010000000
VERSION     DAJOSY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.081"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(345..721)
                     /gene="rnpB"
                     /locus_tag="OHN27_003083"
     ncRNA           complement(345..721)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OHN27_003083"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(754..1899)
                     /gene="garK"
                     /locus_tag="OHN27_003084"
     CDS             complement(754..1899)
                     /gene="garK"
                     /locus_tag="OHN27_003084"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCQ2287475.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1996..2886)
                     /gene="garR"
                     /locus_tag="OHN27_003085"
     CDS             complement(1996..2886)
                     /gene="garR"
                     /locus_tag="OHN27_003085"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCQ2287476.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2916..3686)
                     /gene="garL"
                     /locus_tag="OHN27_003086"
     CDS             complement(2916..3686)
                     /gene="garL"
                     /locus_tag="OHN27_003086"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCQ2287477.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAELAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3702..5036)
                     /gene="garP"
                     /locus_tag="OHN27_003087"
     CDS             complement(3702..5036)
                     /gene="garP"
                     /locus_tag="OHN27_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCQ2287478.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5411..6982
                     /gene="garD"
                     /locus_tag="OHN27_003088"
     CDS             5411..6982
                     /gene="garD"
                     /locus_tag="OHN27_003088"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCQ2287479.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7131..7466
                     /gene="prlF"
                     /locus_tag="OHN27_003089"
     CDS             7131..7466
                     /gene="prlF"
                     /locus_tag="OHN27_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HCQ2287480.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7466..7930
                     /gene="yhaV"
                     /locus_tag="OHN27_003090"
     CDS             7466..7930
                     /gene="yhaV"
                     /locus_tag="OHN27_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HCQ2287481.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(7985..8794)
                     /gene="agaR"
                     /locus_tag="OHN27_003091"
     CDS             complement(7985..8794)
                     /gene="agaR"
                     /locus_tag="OHN27_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCQ2287482.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9043..10323
                     /gene="kbaZ"
                     /locus_tag="OHN27_003092"
     CDS             9043..10323
                     /gene="kbaZ"
                     /locus_tag="OHN27_003092"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCQ2287483.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10346..10819
                     /gene="agaV"
                     /locus_tag="OHN27_003093"
     CDS             10346..10819
                     /gene="agaV"
                     /locus_tag="OHN27_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCQ2287484.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10830..11609
                     /locus_tag="OHN27_003094"
     CDS             10830..11609
                     /locus_tag="OHN27_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCQ2287485.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11599..12477
                     /locus_tag="OHN27_003095"
     CDS             11599..12477
                     /locus_tag="OHN27_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCQ2287486.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQNIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12495..12929
                     /locus_tag="OHN27_003096"
     CDS             12495..12929
                     /locus_tag="OHN27_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCQ2287487.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12926..14059
                     /gene="nagA"
                     /locus_tag="OHN27_003097"
     CDS             12926..14059
                     /gene="nagA"
                     /locus_tag="OHN27_003097"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCQ2287488.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPAGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPEMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTDVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14410..15564
                     /locus_tag="OHN27_003098"
     CDS             14410..15564
                     /locus_tag="OHN27_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HCQ2287489.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15577..16437
                     /gene="kbaY"
                     /locus_tag="OHN27_003099"
     CDS             15577..16437
                     /gene="kbaY"
                     /locus_tag="OHN27_003099"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HCQ2287490.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16604..17080
                     /gene="agaB"
                     /locus_tag="OHN27_003100"
     CDS             16604..17080
                     /gene="agaB"
                     /locus_tag="OHN27_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCQ2287491.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17119..17922
                     /gene="agaC"
                     /locus_tag="OHN27_003101"
     CDS             17119..17922
                     /gene="agaC"
                     /locus_tag="OHN27_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCQ2287492.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17912..18703
                     /gene="agaD"
                     /locus_tag="OHN27_003102"
     CDS             17912..18703
                     /gene="agaD"
                     /locus_tag="OHN27_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCQ2287493.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18704..19459
                     /locus_tag="OHN27_003103"
     CDS             18704..19459
                     /locus_tag="OHN27_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCQ2287494.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATDRFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         4689 a         5075 c         4981 g         4715 t
ORIGIN      
        1 tcttaccctg tgcaccaaaa gcaaaaactt atcgatgcgg gtttgaaact gatggcaacc
       61 gcaaaaatgc ctgatgcgct acgcttatca ggcctacgcc atctctgcaa tatattgaat
      121 ttgcgtgctt ttgtaggcag gataaggcgt tcacaccgca tccggcatga ataaagcgca
      181 ctcaacaatc tgaaacccgc cggaacggtt tattacgtac atcaggtgaa actgaccgat
      241 aagccgcttt cttttgggta tagcgtcgtg gacagtcatt catctttctg cccctccaaa
      301 agcaaaaacc cgccggttta cgctggcggg tttttacgta aatcaggtga aactgaccga
      361 taagccgggt tctgtcgtgg acagtcattc atctaggcca gcaatcgctc actggctcaa
      421 gcagcctacc cgggttcagt acgggccgta ccttatgaac ccctatttgg ccttgctccg
      481 ggtggagttt accgtgccac ggactgttac cagccgcgcg gtgcgctctt accgcaccct
      541 ttcaccctta cctgatcccg cttgcgcggg ccatcggcgg tttgctctct gttgcactgg
      601 tcgtgggttt cccccccagg cgttacctgg caccctgccc tatggagccc ggactttcct
      661 cccctccgcc cgtctccccc gaagaggacg acgacgaagc ggcgactgtc tggtcagctt
      721 cggcgcgcag tatagagggt ttgcgcgccc ttgtcacccc gcgttgcgca ttccaatcgc
      781 cagtgtcgcg gcgatattac gtgaagcacg gcagatattg tcataagccc cgcggaatgc
      841 ttcgtccaac gtacctatgc tggtcaatac gctgaagacc gcatcaatgc catgctgatg
      901 tacaacgcca acatcatcgg tcaggctacc cgcaatgcca atcaccggtt tatggtactt
      961 cttcgccacg tttgcgacac caatcggtac cttcccgtga atactctggc tgtcaatacg
     1021 cccttcaccg gtgatcacca gcgtacaatc gtgaatatgt tcctccagat tcagcgccgt
     1081 agtgacgatt tcaataccac ttttcagttc cgcaccaaga aacgccatta gcgccgcgcc
     1141 cataccaccc gcagctcctg caccggggac atctttcaca tcaacatgca gcgctttttt
     1201 aatgacctcg gcatagtgag agaggttatt gtccagctca acaatcatcg cttcactggc
     1261 tcccttttgt gggccaaaga tgcgcgatgc gccgttatcg cccaccagcg gattggtgac
     1321 atcacaagcg acgcgaatga cgcaatcttt taagcgcgga tcgaggccgg aaatatcaat
     1381 atcattcaga gtattaagac taccgccgcc aaaaccaatt tcattgccgt tggcgtcgca
     1441 taatttcgcc cccagcgcct gtaccatgcc tgcgccgcca tcatttgtag cgctgccgcc
     1501 aatgccgata ataatgtttg tcgcaccgct ctccagcgcc tgcaggatta actcgcctgt
     1561 gccgcgtgaa gtggtcacga gtggatcgcg tttttccgca ggtaccagct ccagcccact
     1621 ggccgccgcc atttcaataa acgcggtttt gccatcgccg gagatccccc aactggcatt
     1681 cactttctcg cccagcggcc ctgtaaccca ggcgtgacgt tcagccccct gggtggctgc
     1741 aatcatcgct tccaccgttc cttcgccacc gtcggcaacc ggaacagaaa cgtactgtgc
     1801 atcaggaaaa atttcccgaa atcctttttc tatcgcctgc gcaacctcgc tggcagataa
     1861 actttcttta taagagtctg gggcgattac gattttcata cctatgcctg ttaccacatg
     1921 acgccggagg gcgtttctct tattcggcct ggattccagg cccggattgc aatacgccat
     1981 ccgggcacga cgtcattaac gagtaacttc gactttcgcc agtttttcgt agtagcacgc
     2041 cagggcgcta tgatccgccg ttcctaaacc atctgctcgc agtgcctgca tcatctccat
     2101 aaccgcagct gtgagcggca gttgtgcgcc gacgccgtga gaagtatcca gcgcattcgc
     2161 cagatcctta atatgcagat caatacggaa gcccggcttg aagttgcggt ccatcaccat
     2221 cggcgctttg gcatccagca cggtactgcc cgccagtcca ccgcgaattg cctgataaac
     2281 caggtccggg ttaacgcccg ctttggttgc cagcgttaac gcttctgaca tcgcagcaat
     2341 attcagcgcc acaatgacct gatttgccag tttggtgacg ttacctgcac cgatttcccc
     2401 ggtatgcacc acggaacccg ccatcgcttt catcaaatca tagtatttgt cgaaaatagc
     2461 cttgtcgccg cccaccatca ctgacagcgt accgtcgatg gctttcggtt caccgccgct
     2521 caccggagca tccagcatat caatgccttt cgctttcagc gcttcgctga tttcacggct
     2581 tgccagcggt gcgatagaac tcatatcgat caataccgta cctggcttcg cgccttcaat
     2641 aatgccattc tcacccagcg ccacctcttt cacatgaggg gagtttggca gcatggttat
     2701 gatgacgtcg cactgttcag cgatcgcttt agccgtagac gctgtttctg cacctgcagc
     2761 aatcacgtca gcaatagctt ctgggttacg gtcagcaacc accagcgagt aacctgcttt
     2821 cagaaggttt ttactcattg gtttacccat aatccccagg ccaataaaac caactttcat
     2881 agtcatatca atcatctctc ttgttgcggt ggtggttatt ttttaaaggt atcagccagt
     2941 ttctgagtgg cagagcggaa gacgccgaga tcgctgccga cagccacaaa cgtcgcgccc
     3001 cattccagat aacgacgcgc atcggcttcg accggcgcga ggataccgct gggtttgccg
     3061 tgcgcgctgg cacggttaaa aatgtgctga attgcttttt gtacatccgg gtgtgatgca
     3121 ttgccgagat ggcctaatgc cgcggccaga tcgctggggc cgacgaagat gccgtctacg
     3181 ccttcggtag cggcaatggc atcgacgtta tctacgccct gctgactttc tatctggacc
     3241 agaatagtga tgttcttgtt cgactgagcg aaataatccg ccacggtgcc aaacatattg
     3301 gcgcggtgag aaacggagac gccgcgaatg ccttccggtg ggtaacgggt tgatgccacc
     3361 gccagctctg cttcctcttt tgtttctaca aaaggaatca ggaagttata gaaaccgata
     3421 tccagaagac gcttaataat taccggctcg ttggtcggca ctcgcactac tggcgcgctg
     3481 gcgctgcctt tcaaggccat taactgcgga ataaacgtgg agatatcgtt tggcgcatgt
     3541 tcgccatcca gcaccagcca gtcaaaccca gccaaaccaa gaacttcagt gctaatcggg
     3601 ttagagagtg ctgaccagca accaatttgt acctgtttcg cagccagtgc ggctttgaat
     3661 ttattcggga aaacatcgtt attcatcgct tatacctttg cttatttctg caattccata
     3721 cgtttaatgt cgccaactac gaagaggtag cagaccatcg ccatcagcgc tgaacatccc
     3781 acgaaaacca gtgctgcatt gaaggagtgc agttcactta ccaggtagcc aatcaccagt
     3841 ggagtgacaa tggaggcaac attgccaaag acgttaaaga cgccgccgca gaggccaaca
     3901 atctctttcg gcgcggtgtc agaaatcacc ggccagccca gcgcaccaaa tcctttgcca
     3961 aagaaagcca gcgccatcag catgaccacc agcgtggtgt tgttggtgta gttacataag
     4021 atgatggtgg aagccagcaa cattcccagc acaatcggta gcttacgtgc cagggtcagg
     4081 gataaaccgc gtttgatcag ataatccgag aagacacctc ccagcacgcc gcccgcaaaa
     4141 ccacacagtg ctggaatcga ggcgaccaga cccactttca gaatcgacat gcctttttcc
     4201 tgcaccagat aaatcgggaa ccaggtgagg aagaaccagg tgatggtgtt gataaaatat
     4261 tgtccgaaaa atacgcccag catcatgcgg ttagagagca attgcttgat gtaatgcagt
     4321 ttgggtccgc ttgctgccgc actgcccggc tttttgtggt ccatatcgac caccgcgcca
     4381 ttttcagaga taaacttcag ctcttccgca gacatacgtg ggtgatctgt cgggttatga
     4441 atcaacttga tccacagcgc cgtcagcaca aaaccaatca cccccataac ggtaaagacg
     4501 tgctcccagc cccaggcgaa agtcagccag ccaagcagcg gcgaaaagag cgccagcgag
     4561 aaatattgcg ccgagttaaa gatggcggag gcagtaccac gttctttcgt cgggaaccag
     4621 gcggcgacaa ttcgggcgtt cgccgggaat gatggcgctt ccgagaagcc gagcataaag
     4681 cgcataaaga acatggagat ccctgcccag gccagcggga acatatcaac aaagccttgc
     4741 aggaaggtga atagcgacca gaaaaagagg ctgtaggtgt aaactttttt cgagccaaac
     4801 ttatcaagca gccagccgcc ggggatttgc atcagcaagt aggcccagcc aaaagcggag
     4861 aagatgtaac ccatcgaaac cgcacttaac tgcaactctt ttgccacttc ggtaccagca
     4921 atagacagcg ttgcacgatc ggcgtagtta acggcggtaa caataaaaat aatcagtaat
     4981 attaaatagc gggtatgcac gcctttcttt ttttcgtcaa cggtgtccag aatcatttta
     5041 tttacctcgg gtacttatgc tgatttttat tattatgggg aaggtgttat ttatgagttt
     5101 catttatgcc gtaacgacaa tgaactcggg aattagtata agcagcgcga gaataataat
     5161 cattgtgcaa atgctaattt aattaatact atttaaatat tattttgagc atatgcacat
     5221 aaggttgcgc gctaaagcac agatttgcgc tttaccttac cgggcggcac tgcaatccct
     5281 gaaatgattg acattgatca catttctgcg tttaaactcc tgacattctt atttcaccca
     5341 atgaagtcat ttatttttaa atgagaccag gtcctcattt taataacccc tggctggaga
     5401 atattgcaca atggccaaca tcgaaatcag acaagaaacg ccaactgcgt tttatataaa
     5461 agttcacgac acagataatg tggcaattat tgttaatgat aatggcctga aagcaggaac
     5521 gcgttttccg gatgggctgg aattaattga acatattccc caggggcata aagtcgcatt
     5581 gctggacatt ccggctaatg gtgaaattat tcgttatggc gaagtgattg gttacgccgt
     5641 gcgtgcaatc ccacgcggaa gctggatcga cgaatcaatg gttgtactac cggaagcgcc
     5701 gccgttacac acgctgccac tggcaaccaa agtcccggaa cccttaccgc cgctggaagg
     5761 atacaccttt gagggctatc gcaatgccga tggcagcgtg ggcaccaaaa acctgctcgg
     5821 tatcaccacc agcgtccact gtgtggcagg cgtggtggac tatgtagtaa aaatcattga
     5881 acgcgatctg ctaccgaaat acccgaacgt cgatggcgtg gtggggctga atcatttgta
     5941 cggttgtggc gtggcgatta acgcaccggc ggcagttgta cctatccgta ccattcacaa
     6001 tatttcgctg aatcctaact ttggcggcga agtaatggtg attggcctgg gttgtgaaaa
     6061 gttgcagcct gagcgcctgc tgactggaac ggatgatgtg caagctattc cagtagaaag
     6121 cgccagcatt gtcagtttgc aggatgaaaa gcatgtcggt tttcagtcca tggtcgagga
     6181 tattttgcag atcgccgaac gccatctaca aaaactgaat caacggcagc gagaaacctg
     6241 cccggcttca gaactggtcg ttggtatgca gtgcggtggc agcgatgcgt tttctggtgt
     6301 aacggcaaac ccggcggttg gctatgcgtc tgatctactg gtgcgctgcg gcgcaacggt
     6361 gatgttttca gaagtaacgg aagtgcgtga cgcgatccat ctgctgacac cacgcgcagt
     6421 gaacgaagag gtcggcaaac ggctgctgga ggagatggag tggtacgata actatctcaa
     6481 tatgggaaaa accgaccgca gcgccaaccc ttcgccgggc aacaagaaag gcggtctggc
     6541 aaacgtggta gagaaggcac tcggctccat tgctaaatcg ggtaaaagcg caattgttga
     6601 agtgctgtcg cccggtcaac gcccgactaa acgcggatta atttacgccg cgacgccagc
     6661 cagcgatttt gtctgtggca cgcaacaggt ggcttcgggt atcacagtgc aagtgtttac
     6721 gaccggtcgt ggtacgccgt acggcctgat ggcggtaccc gtcattaaaa tggcaacccg
     6781 caccgagctg gcgaaccgct ggtttgattt aatggatatt aatgcgggca ccatcgctac
     6841 cggcgaagaa actattgaag aggtgggctg gaagttgttc cactttattc tcgacgtcgc
     6901 cagcgggaag aagaaaacct tctcggatca atgggggctg cataaccagc tggcggtgtt
     6961 taacccggca ccggtgacct gatttctttt ggaacgtccc tcgcaaaaca tggccttagt
     7021 gccatgtttt tattgtttaa agcccccacg tccattaata atgcatttgc attaccttag
     7081 ttcaagctta taatttgagc agaaaacagg ctgtaaaagg acagtgaatc atgcccgcta
     7141 atgctcgctc tcacgctgta ctgaccactg aatcaaaggt cacgatacgc ggacaaacaa
     7201 ctatccccgc gccagtgcgt gaggccttaa aactgaagcc aggccaggac agcattcatt
     7261 acgaaattct gcctggtggg caagtcttta tgtgccgact gggagatgaa caggaggatc
     7321 atactatgaa tgcatttttg cgttttctgg atgcagatat ccagaacaac ccgcaaaaaa
     7381 ctcgtccatt caacattcaa caaggaaaga aacttgtcgc tggcatggac gtcaacattg
     7441 atgatgagat tggcgacgac gaataatgga ttttccacaa agggttaatg gttgggcgct
     7501 atatgctcat ccctgttttc aggaaaccta cgatgcttta gttgccgaag tcgaggcatt
     7561 aaagggaaaa gatcctgaaa attatcagag aaaagctgcc acaaagttat tggcggtggt
     7621 ccataaagtg attgaggagc atatcacggt caatccatca tcaccggcat tccgtcatgg
     7681 caagtcgtta ggctctggga aaaataaaga ctggtcacgg gtaaaatttg gtgctggtcg
     7741 ctatcgtctc ttctttcgtt acagtgaaaa agagaaagtc atcattctgg gatggatgaa
     7801 cgatgaaaac acgctgcgca cctacggtaa aaaaacagat gcctataccg tattcagtaa
     7861 aatgttaaaa agaggacatc ctcctgccga ctgggaaagc ctcacccaag agacagaaga
     7921 aaaccattga tggtgttcac aatgcccgcc gaacgtattc gtgttgacca gcgggcaccc
     7981 tttctcactc cccgaccaga atcacttcaa ccccagcctt tcgcagtcct tccagactat
     8041 ccgcaggaat gccttcatca acaatgatca tgtcgatacg ttgagtatca atgatcttat
     8101 gtaaactgga acgattgaac ttactggaat cggtgactac gatgatccgt tccgcaactt
     8161 cgcacatccg gcggtttaaa cgggcttcat cttcattatg cgtgctgacg ccgcgctcca
     8221 gatcgatcgc atctacacca agaaacagca tatcgaagtg gtaattttgc agcgattgtt
     8281 cagcctgatc gccgtaaaaa gattgcgact gacggcgcaa atgcccgccg gtcatcagca
     8341 gctcaacgcc ttccgcttcc agcaaagcat tagccacgtt cataccgttg gtcatcgcaa
     8401 ttacgtcagt gtgcttgcgc atcagacgag caatctcaaa agtggtggtc ccggaatcga
     8461 ggatcacccg atgacctggc tgaatcaact caacggcagc tttcgcaacg ctgcgtttca
     8521 tcgcggtgtt cagtgcgctt ttatcttcca ctgatggctc gactgacggc gtcgtgctat
     8581 cgcagatcaa cgcgccacca taggcacgca cagcgatccc ctgcttttcc agaaacgcca
     8641 gatcgttgcg gatcgtcacg gtagatacgc catacaatgc cgacagatcg ttaacctgca
     8701 cactcccttg ctgtcgcaga cgctgaatga tctgttctcg tcgctcgctg gtgcctgtca
     8761 ctcgcttctc acctgaagcg tcggtattac tcatagtaag tcctttcgta aaactttcgt
     8821 ttcatttcgt tttgcctatt aacgcctttc tattaagcaa atgcaagccc accttgccca
     8881 ttgacgcaag ctactctcgt ttcagtgact ttcattatgt ttcttttgtg aatcagatca
     8941 gaaaaccatt atctttcgtt ttatttttat ctcaccatga cgcagtatca actgaaacaa
     9001 aacgaaagat taatatcgca gtaatctgaa ctggagagga aagtgaaaca tctaacagaa
     9061 atggtgagac agcacaaagc gggcaaaaca aatgcaattt atgccgtttg ttccgcacat
     9121 ccgctggtgc tggaagctgc aatccgctac gccagtgcaa accaaacgcc gttactgatt
     9181 gaagcaacct ccaatcaggt agaccagttc ggcggttata ccggaatgac gcccgccgat
     9241 tttcgcggct ttgtttgtca gctcgccgac tcgttgaatt tcccgcagga tgcgttgatt
     9301 ctgggtggtg accatctggg gccaaaccgc tggcaaaacc tgccagccgc tcaggcaatg
     9361 gccaatgccg atgatttgat taaaagctac gttgcggcag gattcaaaaa aatccacctt
     9421 gattgcagca tgtcctgtca ggacgatcca attcccttaa ctgatgacat cgtggctgaa
     9481 cgcgccgccc gtctggcgaa agtggcggaa gaaacctgtc ttgaacactt tggcgaagcc
     9541 gatctggagt atgtcattgg taccgaagtg ccggtacctg gcggcgcgca tgaaacctta
     9601 agcgagctgg cggtcaccac gccggatgcc gcccgcgcca cgctggaagc ccatcgtcac
     9661 gcctttgaaa agcaaggttt gaatgccatc tggccacgca tcattgccct ggtggttcaa
     9721 cccggcgtcg aattcgatca caccaacgtt attgattatc agcccgccaa agcgagcgcc
     9781 ttaagccaga tggtcgaaaa ctacgaaacg ctgattttcg aagcgcactc taccgattat
     9841 caaacgccgc aatcgctgcg ccagctggtg attgaccact ttgccattct gaaagttggc
     9901 ccagcgctga ccttcgccct gcgtgaagct ctgttctctc tggcggcgat tgaagaagaa
     9961 ctggtgccag cgaaagcctg ttccggtctg cgtcaggtgc tggaagacgt gatgctcgac
    10021 cgcccggaat actggcaaag ccactaccac ggtgacggca acgcgcgtcg tctggcgcgt
    10081 ggttatagct actcggatcg cgtgcgctat tactggccgg acagccagat tgatgacgct
    10141 ttcgctcatc tggtacgtaa tctggcggat tcaccaattc cgctgccgct gatcagccag
    10201 tatctgccgc tgcagtacgt gaaagttcgc tccggcgagc tgcagccaac gccacgggaa
    10261 ctcattatca accatattca ggacatcctg gcgcagtacc acacagcctg tgaaggccaa
    10321 taagcaaaac aaagaggaac acgctatgcc aaatattgtt ttaagccgga ttgatgaacg
    10381 cttgattcac ggtcaggtcg gcgttcaatg ggtcggattt gcgggggcaa atctggtgct
    10441 ggtagccaac gatgaggttg ccgaagatcc ggtacaacaa aacctgatgg aaatggtact
    10501 ggcagaaggg atcgccgtac gtttctggac gctgcaaaaa gttatcgaca acattcatcg
    10561 cgccgccgat cgacagaaaa tcctgctggt ttgtaaaaca cccgccgatt tcctgacgct
    10621 ggtgaaaggt ggcgttccgg tgaatcgcat taacgttggc aatatgcact acgccaatgg
    10681 caaacaacaa atcgccaaaa cggtttctgt ggatgcgggc gatatcgcag catttaacga
    10741 cctgaaagcc gctggggtgg aatgcttcgt tcagggcgtc ccgacagagc ctgctgtgga
    10801 cctctttaaa ttactttgag ggattcatca tggaaatcag cctgttgcag gcatttgcgt
    10861 tgggcattat cgcctttatc gctggcctgg atatgtttaa cggcctaaca catatgcacc
    10921 gcccggtggt cctcggcccg ttggtcgggc tggtacttgg cgatctgcat accggaattt
    10981 taaccggcgg tacgctggaa ctggtgtgga tggggctggc cccgctggcg ggcgcacagc
    11041 cgcctaacgt gattatcggt actatcgtcg gcacggcgtt tgccattact actggcgtga
    11101 aacccgatgt cgcagtcggt gtcgccgtac ctttcgctgt cgcagtacag atggggatta
    11161 ccttcctgtt ctcggtgatg tccggcgtga tgtctcgctg cgaccggatg gcggagaacg
    11221 ccgatactcg cggcattgaa cgcgtgaact atctggcact gctggcactc ggcactttct
    11281 attttctctg cgctttcctg cctatctact tcggcgcgga acatgcaaaa accatcattg
    11341 atgtcctgcc gcaacgatta atcgacggcc tcggcgtcgc gggcggcatc atgccagcaa
    11401 tcggttttgc cgtgctgctg aaaatcatga tgaaaaacgt ctacatcccc tacttcatcc
    11461 tgggctttgt tgccgcagcc tggctcaagt taccggtgct ggctattgct gccgctgcgc
    11521 tggcgatggc gctgatcgac ctgctgcgta aatctcctga accgactcaa cctgcggcac
    11581 agaaagagga atttgaagat ggcatctaat caaaccaccc tgccgaacgt cagtgaaaac
    11641 gaagaaacat tgctgactgg cgtcaatgaa aacgtgtatg aagatcagaa cattggcgcg
    11701 gagctgacga aaaaagatat caaccgcgtc gcctggcgtt ccatgctgtt acaagcgtct
    11761 tttaactacg aacgtatgca ggcttctggc tggttgtacg gtctgctgcc tgctctgaaa
    11821 aagatccaca ctaataaacg cgacctggcg cgcgccatga aggggcatat gggtttcttc
    11881 aatacccatc cgtttctggt gacatttgtt atcggcatta tccttgcgat ggagcgttct
    11941 aagcaggacg ttaacagtat tcagagcacc aaaattgccg tcggtgcgcc gctcggcggg
    12001 attggcgatg caatgttctg gctaacgcta ctgccgattt gtggcgggat tggagccagt
    12061 ctggcattgc aaggctccat tcttggcgca gtcgtcttta ttgtgctgtt caacgtggta
    12121 cacctggggc tgcgttttgg tctggcgcat tatgcttacc gcatgggcgt ggcggcgatt
    12181 ccactgatta aagcgaatac caaaaaagtc ggccatgctg cgtctatcgt tgggatgacg
    12241 gtaatcggtg cgctggtggc aacctatgtt cgtttaagca ccacgctgga aatcacggcg
    12301 ggcgacgcag tggttaagtt acaggctgat gttatcgaca aactgatgcc ggccttctta
    12361 ccgttggtct acaccctgac catgttttgg ttggtacgcc gcggctggag tccgctgcgt
    12421 ctgattgcag ttaccgtggt tctcggcatc gtcggtaaat tctgccattt cctttaaata
    12481 caatgaggta ttagatgtta agtattattt tgacagggca tggcgggttt gccagcggca
    12541 tggaaaaagc aatgaagcaa attctcggtg agcaatcgca gtttatcgcc atcgattttc
    12601 cggaaacctc aagcaccgcg ctgcttacat cgcagcttga agaggccatc gcccaactgg
    12661 attgtgaaga cggcattgtt tttctgacgg atttgctggg cggcacgccg tttcgcgtag
    12721 cttcaacgct ggcgatgcaa aaaccgggct gtgaagtgat caccggcacc aatttgcaac
    12781 tgctactgga gatggtgctg gagcgcgaag ggttaagcgg tgaagaattc cgtgtacagg
    12841 cgctggagtg tggacaccga gggctgacca gtctggtgga cgagttaggc cgctgccatg
    12901 aagaatgtcc ggtcgaggaa ggaatatgac acacgttctg cgcgccagaa ggctgctgac
    12961 tgaagaggga tggctcgatg accatcagtt gcgtattgct gacggtgtca tcgcagcaat
    13021 cgaaccgatt ccagcgggcg tgactgaacg cgatgcggaa ctgctctgcc ctgcttacat
    13081 cgacacccat gtacacggtg gtgcgggcgt tgatgtaatg gatgacgcgc cggatgtact
    13141 cgacaagctg gcaatgcaca aggcacgcga aggtgtcggc agttggttac cgaccaccgt
    13201 aaccgcgccg cttaatacca ttcatgcggc gctgaaacgt attgctcaac gttgccaacg
    13261 cggcggacct ggtgcgcaag tgctggggag ttatctcgaa ggaccgtact tcacgccgca
    13321 gaataaaggc gcgcatccgc cggagttgtt tcgcgagctt gaaattgccg agctggatca
    13381 gttgattgcc gtttctcagc acaccttacg tgtggtagcg ctggcaccgg aaaaagaagg
    13441 ggcattgcag gccatccgcc atcttaaaca gcaaaacgta agggtgatgc tggggcatag
    13501 cgcggcgacc tggcaacaaa ctcgcgccgc gtttgatgct ggtgccgacg gcctggtgca
    13561 ttgctataac gggatgacag gtttacatca ccgcgaaccg gaaatggttg gcgcgggatt
    13621 aacggacaag cgcgcctggc tggaactgat agccgatggt catcatgtgc atccggcggc
    13681 gatgtcgctg tgttgttgct gtgcaaaaga gagaatcgtg ctgatcaccg acgcgatgca
    13741 ggcagccggg atgccggatg gtcgctatac gttatgtggc gaagaagtgc agatgcacgg
    13801 tggcgttgtc cgtaccgcgt ccggtgggct ggcgggcagt acgctgtctg ttgatgcggc
    13861 agtgcgcaac atggtcgagt tgacggacgt aacgcctgcg gaagccattc atatggcatc
    13921 gctgcatccg gcgcgaatgc tgggtgttga tggtgttctg ggatcgctta aaccgggcaa
    13981 acgcgccagc atcgttgcgc tggatagcgg gctgcatgtg caacaaatct ggattcagag
    14041 tcaattagct tcgttttgat agtttgctcc tttattgggc cttcacttcc cccgtaaggc
    14101 ctttcttttt ctttcgtttt gatctgtgca gcggtgtcgg atgcgacgct aacgcgtctt
    14161 atgcgaccta cggtacgccg catccagcaa tcggtgaaca tagcgacgtc attacgcaat
    14221 tctttaatct tcccttgtct tttctttcaa tgatcacaga tttcgtttta tttccttttt
    14281 ctccattgaa ctttcggttt cttttctata gattttaatc aacgaaagac atcaccaagt
    14341 gaaatgaaac gaaaggcaag tgaaagcgac aacgcccgac gtcaagttca tcagactaag
    14401 gattgagtta tgccagaaaa ttacacccct gctgccgccg caaccggtac atggactgaa
    14461 gaagagatcc gccatcagcc tcgcgcatgg atccgttcac tcaccaacat cgacgcgcta
    14521 cgttccgcgc tcaataactt ccttgaaccg ttactgcgca aagagaatct gcggatcatc
    14581 ctgaccggag ccggaacctc agcatttatc ggtgacatca tcgctccgtg gctcgccagc
    14641 cataccggta aaaacttcag cgccgtaccg accaccgatc tggtcactaa tccgatggac
    14701 tacctgaatc cagctcatcc gctgctgttg atctccttcg gtcgatccgg caacagcccg
    14761 gaaagcgtcg ctgccgtgga actggcaaat caatttgtac cggaatgcta tcacctgccg
    14821 atcacctgca acgaagcggg cgctctttac caaaacgcga tcaacagcga taacgcgttt
    14881 gccctgctga tgcccgcaga aacgcacgat cgcggctttg cgatgaccag cagcattacc
    14941 accatgatgg ccagctgcct cgcggttttc gcacctgaga cgatcaacag ccaaaccttc
    15001 cgcgacgtgg cggatcgttg ccaggcgatc ctgacctcac tgggcgattt cagcgaaggt
    15061 gtgtttggtt acgcaccgtg gaaacggatc gtttatctcg gtagcggtgg cttacagggc
    15121 gcagcacgcg agtcggcgct gaaagtgctg gaactgacgg cgggtaaact ggcggccttt
    15181 tatgattctc caaccggatt ccgtcatggc ccgaaatcac tggtcgataa cgaaacgctg
    15241 gtggtggtat ttgtctcaag ccacccttac acccgtcagt atgatcttga tctgctggct
    15301 gaacttcgcc gtgacaacca ggcaatgcgt gtaatcgcca tcgccgcgga aagcagcgac
    15361 atcgtcgctg ccggtccaca tattatcctg ccgccgtcac gtcactttat cgacgttgag
    15421 caggcatttt gcttcctgat gtacgcccag acgtttgcac tgatgcagtc gctgcacatg
    15481 ggcaatacgc cggatacccc atcagccagt ggcaccgtta accgcgtggt gcaaggcgta
    15541 atcattcatc cgtggcaggc ataagaggat cgcattatga gcattatctc caccaaatat
    15601 ctgttacagg acgcccaggc caatggctac gcggtgcctg cctttaacat tcataacgcc
    15661 gagacgatcc aggcgatcct cgaagtgtgc agtgaaatgc gatcgccggt gatcctcgcc
    15721 ggaacgccgg ggacctttaa acatatcgcg ctggaagaga tctacgccct gtgtagcgcc
    15781 tattccacaa cctacaacat gccactggcg ctgcatctcg accaccacga atcgctggat
    15841 gatattcgcc gtaaagtcca cgcaggcgtg cgcagtgcga tgatcgacgg cagccacttc
    15901 ccgtttgccg agaacgtgaa gctggtgaaa tcggttgttg acttctgcca ctcacaagat
    15961 tgcagcgtgg aagcagaact gggccgcctg ggcggtgttg aagatgacat gagcgttgac
    16021 gccgaaagtg cattcctgac cgatccacaa gaagctaaac gctttgtcga actgactggc
    16081 gtcgacagcc tggcggtagc gattggtacg gcgcacggct tatacagcaa aacgccgaag
    16141 attgatttcc agcggctggc ggaaattcgt gaagtggtgg atgttcctct ggtgctgcat
    16201 ggtgccagcg atgttccgga tgaatttgtc cgtcgcacta ttgaacttgg cgtcacaaaa
    16261 gtgaacgttg ccacagaatt aaaaatagcc ttcgctggcg cggttaaagc ctggtttgcg
    16321 gaaaatccgc agggtaatga tcctcgttat tatatgcgcg tcggaatgga tgcgatgaaa
    16381 gaagttgtca gaaataaaat taatgtctgt ggttcagcga atcgaatttc agcataatca
    16441 ttagtttttt atgatttatc ccaatgtact tcccgattaa tatcggggag tgccttaatg
    16501 gaaaaggaga taactaaacc ttaataaata catcagtaca atatcgcaac aataatatat
    16561 ttaaaaaaat tatattattc aactttatgg tgaggattac acaatgagca gtccaaatat
    16621 tctcttaacc cgtattgata accgtctggt tcatggccag gttggcgtga cctggacatc
    16681 caccatcggt gcaaatctgc tggtagtcgt ggatgatgtt gtcgctaacg atgatattca
    16741 acagaaatta atgggtatta ccgcggaaac ctacggcttt ggcattcgtt tctttactat
    16801 cgaaaaaacc attaacgtca tcggcaaagc tgcaccgcat cagaagatct tcctgatttg
    16861 ccgtacgcca caaacggtac gtaaattggt agaaggtggc attgacctga aagatgtcaa
    16921 cgtcggcaat atgcatttct cggaagggaa aaagcaaatc agcagtaaag tttatgtcga
    16981 tgaccaggat ctcgcggact tacgttttat taaacaacgt ggcgtgaatg ttttcattca
    17041 ggacgtccct ggcgatcaaa aagaacaaat ccctgactaa atctaaaatc gccttaatat
    17101 tggtttgagg taataaaaat gcatgaaata accctacttc agggattatc cctggcggcg
    17161 ttagtttttg ttctggggat tgatttttgg ctggaagcct tatttttatt ccgcccgata
    17221 atcgtttgta ccctaactgg cgctattctc ggtgatattc agactggctt aattaccggt
    17281 ggtctgacag agttggcttt cgccggatta acccctgcag gtggtgttca gccgcccaac
    17341 ccgattatgg cgggtctgat gaccaccgtc attgcatggt ctacgggcgt tgatgccaaa
    17401 acagcaattg gtcttggcct gccgtttagt ttgttaatgc agtacgtcat tctgttcttc
    17461 tattccgctt tctcattatt tatgaccaaa gccgataaat gcgcgaaaga ggcggatacg
    17521 gcagcgtttt cccggcttaa ctggacaacg atgctcatcg tcgcttcagc gtatgcggtg
    17581 attgctttcc tctgtactta cctggcacag ggggcgatgc aggcgctggt gaaagcgatg
    17641 cccgcctggc tgacccacgg ctttgaagtg gcaggcggta ttctgcctgc cgttggtttt
    17701 ggcttgctgc tgcgcgtaat gttcaaagcg caatatatcc cttacctgat cgccggtttc
    17761 ctgtttgttt gctacatcca ggtcagcaac ctgttgccgg ttgccgtact gggcgcaggc
    17821 tttgcggtgt atgagttttt caatgcgaaa tcccggcagc aagcgcaacc gcagcccgtt
    17881 gccagtaaaa atgaagaaga ggactacagc aatgggatct gaaatcagta aaaaagatat
    17941 cacccgtctg ggctttcgtt cgtcgctgct gcaagcgagc tttaactacg aaaggatgca
    18001 ggcgggcggt tttacttggg cgatgttgcc gatcctgaaa aagatttata aggacgacaa
    18061 accgggctta agcgcggcga tgaaagataa cctcgaattt attaataccc acccgaatct
    18121 ggtcggattc ctgatggggt tattaatttc gatggaagaa aaaggagaaa accgcgacac
    18181 cattaaaggc ctcaaagtgg cactgtttgg cccaatcgcc gggattggcg atgcgatttt
    18241 ctggtttact ttgttgccga ttatggcggg aatttgctca tcatttgcca gccagggaaa
    18301 cctgctgggg ccgattctat ttttcgccgt ttacctgctt atctttttcc tgcgcgtcgg
    18361 ctggacccac gtcggttatt cagtcggcgt gaaggcgatc gataaagtgc gagagaactc
    18421 gcagatgatt gcccgttcgg caaccatcct cgggatcacg gtaatcggcg ggctgatcgc
    18481 ttcgtatgtg catattaacg tggtgacatc gtttgccatc gacagtaccc acagcgtcgc
    18541 gctgcagcag gatttcttcg ataaagtctt cccgaacatt ttaccgatgg cctacaccct
    18601 gctgatgtat tacttcctgc gggtgaaaaa agcgcatccg gtgctgttaa tcggcgtgac
    18661 ttttgtgctc tctattgttt gttccgcatt cggcattttg taaatggaac gaggcactgc
    18721 gtctggcggt gcctctttac ttaaggaatt tcatcctgtg caaacccttc agcaagttga
    18781 aaactatacg gcgttaagtg aacgtgccag cgaatattta ttggccgtga tccgtagcaa
    18841 accggatgcc gtgatttgcc tggcgaccgg agccacgcca ttactgacgt atcattatct
    18901 ggtagaaaaa atccaccagc agcaggttga tgtcagccag ctcaccttcg tgaagctcga
    18961 cgaatgggtg gatctgccat taacgatgcc tggtacctgc gaaactttcc tgcaacagca
    19021 tatcgtgcag ccgctggggc tacgtgaaga ccagctcatc agctttcgct ccgaagagat
    19081 aaatgagaca gagtgcgaac gggtaacgaa cctgattgcg cgcaaaggcg gtctggattt
    19141 atgcgttctc ggattgggga aaaacggtca tcttgggctg aacgaaccgg gagaaagcct
    19201 gcaaccggcc tgccatatca gtcaacttga tgccagaaca cagcaacatg agatgttaaa
    19261 aaccgcgggt cgccccgtga ctcgtgggat caccttaggc ctgaaggata ttctcaatgc
    19321 ccgcgaagtt ttgttactgg tgactggcga aggaaagcag gatgcgacag atcgttttct
    19381 cacggctaaa gtctctaccg ctatcccggc ttcattttta tggctgcaca gtaactttat
    19441 ttgtttaatt aacacctaac
//
LOCUS       DAJOSY010000082        18984 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJOSY010000082 DAJOSY010000000
VERSION     DAJOSY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.082"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..768
                     /locus_tag="OHN27_003104"
     CDS             <1..768
                     /locus_tag="OHN27_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCQ2287495.1"
                     /translation="NSGGDATQTAAILSGLTAEQSTIERALAMNWLAKYMATMPPVVL
                     PAPAGAWAKHKLTGGGEYWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIP
                     VTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDALYLDEITLTSEQDAVLRYGQVEVPL
                     PPGADVERTTWGISVNKPNAAKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLR
                     FSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGMQVK"
     gene            769..2418
                     /locus_tag="OHN27_003105"
     CDS             769..2418
                     /locus_tag="OHN27_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCQ2287496.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            2423..3199
                     /locus_tag="OHN27_003106"
     CDS             2423..3199
                     /locus_tag="OHN27_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCQ2287497.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            3474..6323
                     /gene="rcsC"
                     /locus_tag="OHN27_003107"
     CDS             3474..6323
                     /gene="rcsC"
                     /locus_tag="OHN27_003107"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCQ2287498.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESSMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(6523..7173)
                     /gene="rcsB"
                     /locus_tag="OHN27_003108"
     CDS             complement(6523..7173)
                     /gene="rcsB"
                     /locus_tag="OHN27_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCQ2287499.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(7190..9862)
                     /gene="rcsD"
                     /locus_tag="OHN27_003109"
     CDS             complement(7190..9862)
                     /gene="rcsD"
                     /locus_tag="OHN27_003109"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCQ2287500.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGIMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            10600..11727
                     /gene="ompC"
                     /locus_tag="OHN27_003110"
     CDS             10600..11727
                     /gene="ompC"
                     /locus_tag="OHN27_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCQ2287501.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNGSYISNGVARNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLG
                     WANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            11839..12894
                     /gene="apbE"
                     /locus_tag="OHN27_003111"
     CDS             11839..12894
                     /gene="apbE"
                     /locus_tag="OHN27_003111"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCQ2287502.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYIDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            12968..14032
                     /gene="ada"
                     /locus_tag="OHN27_003112"
     CDS             12968..14032
                     /gene="ada"
                     /locus_tag="OHN27_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCQ2287503.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            14032..14682
                     /gene="alkB"
                     /locus_tag="OHN27_003113"
     CDS             14032..14682
                     /gene="alkB"
                     /locus_tag="OHN27_003113"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCQ2287504.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVIWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            14758..16401
                     /gene="yojI"
                     /locus_tag="OHN27_003114"
     CDS             14758..16401
                     /gene="yojI"
                     /locus_tag="OHN27_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCQ2287505.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPHPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            16619..18265
                     /gene="mqo"
                     /locus_tag="OHN27_003115"
     CDS             16619..18265
                     /gene="mqo"
                     /locus_tag="OHN27_003115"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCQ2287506.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(18415..18903)
                     /gene="eco"
                     /locus_tag="OHN27_003116"
     CDS             complement(18415..18903)
                     /gene="eco"
                     /locus_tag="OHN27_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCQ2287507.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLSGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         4586 a         4622 c         5066 g         4710 t
ORIGIN      
        1 aatagcggtg gtgacgctac ccagacggcc gctattttaa gtggtttgac cgctgagcaa
       61 tccactattg agcgcgcgct ggccatgaac tggctggcga aatatatggc gacaatgcct
      121 ccagttgttt tgcctgcgcc tgcgggcgca tgggccaaac ataagttaac tggagggggc
      181 gaatactggc gttgggttgg tcagggtgtg ccggacattc tctcttttgg tgacgaatta
      241 tcgccgcaaa atgtgcaggt ccgctggcgt gaaccggcaa aaacggctca acaaagtaac
      301 attccggtaa ccgttgaacg ccagttgtat cggcttatcc ccggtgaaga agagatgagc
      361 tttactctgc aaccggtgac cagcaatgag attgacagcg atgcgctgta tctcgatgaa
      421 atcacgctta ccagcgagca ggatgcagtt ctgcgctatg gtcaggtgga agtaccgctc
      481 ccgccgggag ccgacgttga gcgcacaaca tggggcattt cggtcaataa acccaacgcc
      541 gcgaaacagc aggggcaatt gctggaaaaa gcgcgtaatg aaatgggcga actggcctat
      601 atggtgccgg tgaaagaact gacgggaacg gtcactttcc gccatttgct gcgcttctcg
      661 caaaaagggc aattcgttct gcctcctgct cgttatgtgc gttcctatgc acctgcgcag
      721 caaagtgttg cggcagggag tgaatggacc gggatgcagg tgaaataagt gaactggcgc
      781 agaatcgtct ggctattggc gttggttact ctgccaacgc ttgcggagga gacgccgtta
      841 caactggtgc tgcgcggtgc gcagcacgat caactgtatc agctgtcgtc atcaggcgtg
      901 accaaagtct cagcattacc tgactcgctc accacgccgt tagggagttt gtggaaactg
      961 tatgtttacg cctggctgga ggatacccat caaccggagc agccctacca gtgtcgcgga
     1021 aactccccgg aagaggttta ttgttgtcag gctggggaga gcataacgcg cgataccgcg
     1081 ctggtacgtt cctgtgggct ctattttgcg ccgcagcggt tgcatattgg cgcagatgtg
     1141 tgggggcaat actggcaaca gcgtcaggca cctgcgtggt tagcgtctct tacaacgctg
     1201 aagccagaaa cctcggtaac ggttaaatcg ctgcttgatt cattagcgac gttacctgcg
     1261 caaaacaaag ctcaggaagt cttactggat gtggtgctgg acgaggcgaa aatcggtgtc
     1321 gcatccatgc tcggtagtcg ggtacgggtg aaaacctgga gctggtttgc cgatgataaa
     1381 caggaaatcc gccagggtgg ttttgctggc tggttaaccg atggtacacc gctgtgggtt
     1441 actggcagtg gcaccagcaa aacggtacta acccgttatg cgacggtgct taatcgggtt
     1501 ttgccagtgc cgacgcaggt tgccagtggg caatgtgtag aggtggagct gtttgcccgc
     1561 tatccgctga agaaaatcac agcggaaaag agtacgacgg cagtaaaacc cggcgtactt
     1621 aatgggcgct atcgtgtgac gtttactaat ggtaaccaca ttacgtttgt cagccacggt
     1681 gagacgacct tattgagcga aaagggcaaa ctgaaattgc agtcgcatct cgatcgtgaa
     1741 gagtacgttg ccagagttct ggatcgcgag gcgaaaagta cgccacctga ggcagcgaaa
     1801 gccatgaccg tggcgatacg tactttttta cagcaaaacg ccaatcggga aggtgattgc
     1861 ctgaccatcc ccgacagcag cgccacgcag cgtgtttctg cttcgccagc gaccacgggg
     1921 gcgcgaacga tgaccgcctg gacgcaggat ctcatctatg caggcgatcc ggttcattat
     1981 catggcagtc gcgctaccga agggacactt tcctggcggc aagccacggc ccaggcgggg
     2041 cagggggagc gttacgacca gatactcgcg tttgcttatc ccgacaacag ccttagccgc
     2101 tggggagctc cacgctcaac ctgccagtta ttacccaaag cgaaagcctg gctggcgaaa
     2161 aaaatgccgc agtggcggcg tatattacaa ggcgagacgg ggtacaacga accagacgtg
     2221 tttgcggtct gtcgtttagt ctcaggtttc ccctataccg atcggcagca gaaacggctg
     2281 tttattcgta acttctttac gcttcaggat cggctggatt taacccatga gtatctgcac
     2341 ctggcctttg atggttatcc caccgggctt gatgagaact atatcgaaac gctgacccga
     2401 caattattaa tggactaatg ctatgcgaaa gatttttctt ccgttgttac tggtggcact
     2461 ttcgcctgtc gctcacagtg aaggtgtgca ggaagtcgag attgacgcac cgctctctgg
     2521 ctggcatccg gcagagggcg aagatgccag tttttcacaa tcgatcaatt acccggcgtc
     2581 gtccgtcaac atggcagatg atcaaaatat ctcagcgcag atccgcggca aaataaagaa
     2641 ttatgctgcg gcgggtaaag ttcagcaggg ccggctggtg gtcaacggtg ccagtatgcc
     2701 gcagagaatt gaatccgatg gttcatttgc acgcccttat attttcactg aaggcagcaa
     2761 cagcgtgcag gtcatcagcc cggatgggca aagccggcaa aaaatgcagt tttactcaac
     2821 gccgggtaca ggaacgattc gtgcacgttt acggctggtt ctctcgtggg atacggacaa
     2881 taccgatctc gatctccatg ttgtcacgcc cgatggcgaa cacgcctggt acggtaacac
     2941 cgtgctgaaa aacagtggtg cactggatat ggatgtcacg acggggtacg gacccgagat
     3001 tttcgccatg ccagcaccag ttcacgggcg ctatcaggtg tatatcaact actatggtgg
     3061 gcgcagcgaa acggaattaa ccaccgccca actgacgctg atcaccgatg aaggatcggt
     3121 caatgagaaa caggaaacat ttattgtacc gatgcgtaat gctggcgagc tgacgctggt
     3181 gaaaagtttt gactggtgat ccatcccttc acacaacctg gctctctctt ttttcagata
     3241 agactgctgg gcgatctgcc gacgccagga tataaccatt aacaagcgtc ggcagattgg
     3301 tcatttctta tttttcgtac tgagaactgt ccatctactc cggctttcct ggttaaggca
     3361 atggtttcta agcgatacgc attattgcaa tggtgacaga acattgcggt aacacgcttt
     3421 taccgctacc ttaaccacac tccatcggtc acctgaggcg gagcttcgcc cctttgaaat
     3481 accttgcttc ttttcgtaca accctgaaag cctcgcgcta catgttcaga gcattggcgt
     3541 tagtgctctg gctgttgatt gctttttcat ccgtttttta catcgttaat gcgttacatc
     3601 agcgagaatc ggaaattcgt caggaattta atctgagttc cgatcaggct cagcgcttta
     3661 ttcaacgcac ctctgatgtg atgaaagagc tgaagtacat cgccgaaaat cgcttatcgg
     3721 cagaaaacgg tgtgctttcc ccgcgtggac gagaaacgca ggcggatgtg cctgcgtttg
     3781 aaccgctgtt tgccgactcc gattgttccg caatgagtaa cacctggcga ggttctctgg
     3841 agtcattggc gtggtttatg cgctactggc gcgataattt ttctgcggct tacgatctca
     3901 accgggtatt tttaatcggc agtgataacc tctgcatggc caatttcggt ctgcgtgata
     3961 tgccagtgga acgcgatacc gcgttgaaag ctttgcatga acgcatcaat aaatatcgaa
     4021 atgcaccaca agatgatagc ggcagtaacc tctactggat cagcgaaggt ccgcgccctg
     4081 gcgtcgggta tttttacgcg ttgacgccag tttatctggc gaaccggttg caggcgcttt
     4141 tgggtgtcga gcagaccatc cggatggaga actttttctt accgggtacg ttgccgatgg
     4201 gggttaccat tcttgatgaa aatggtcata ccctgatttc gcttaccgga ccagaaagta
     4261 aaattaaggg cgatcctcgc tggatgcagg aacgctcctg gtttggctat acggaagggt
     4321 tccgggagct ggtgctgaag aaaaatctgc caccctcatc gctaagcatc gtgtattcgg
     4381 tgccggttga taaggtgctg gaacgcattc gcatgttgat ccttaacgca attttgctga
     4441 atgtgcttgc cggagctgca ttgtttactc tcgcacggat gtacgagcga cgtattttca
     4501 ttccggcgga aagcgacgcc ctgcgactgg aagaacatga gcagttcaat cgcaagattg
     4561 tcgcctccgc gccagtgggt atctgcattt tgcgtaccgc tgatggcgtc aatattttaa
     4621 gtaacgaact ggcgcatacc tatctcaata tgcttacgca tgaggaccgc caacgactga
     4681 cgcaaattat ctgtgggcag caggtcaatt ttgttgatgt cctgaccagc aacaatacca
     4741 atctgcaaat cagcttcgtc cattcgcgct atcgtaatga aaacgtggcc atttgtgtgc
     4801 tggtggatgt ttcttcgcgc gtgaagatgg aagagtcgtt gcaggagatg gcacaagcag
     4861 cggaacaggc gagccagtca aaatcgatgt tccttgccac cgtcagtcat gagctgcgaa
     4921 cgccgctgta tggcattatc ggtaacctgg atctgttgca aaccaaagag ttaccgaaag
     4981 gcgtcgatcg gctggtgacg gcaatgaaca actcttccag cctgttgttg aaaattatca
     5041 gcgatattct cgatttctcg aagattgaat cggaacagtt gaagatcgaa ccgcgtgagt
     5101 tttcaccgcg tgaagtgatg aaccacatca ccgccaacta tttaccgctg gtggtacgca
     5161 agcagttagg cttgtactgc tttattgaac cggatgtgcc agtggcctta aatggcgacc
     5221 cgatgcgttt acagcaggtc atctccaacc tgttgagtaa cgccataaaa ttcaccgata
     5281 ccggctgtat agttttgcat gtccgcgctg atggcgatta tctctctatc cgtgttcgcg
     5341 ataccggcgt ggggattccg gcgaaagaag tggtgcgctt gtttgatccc ttcttccagg
     5401 tcggaacggg cgtacagcga aacttccagg ggaccggtct gggtctggcg atttgtgaaa
     5461 aactgatcag catgatggac ggcgatatct cggtagattc agaaccggga atgggcagcc
     5521 agtttaccgt gcgtattccg ttgtacggcg ctcagtaccc gcagaaaaaa ggcgtggaag
     5581 ggttgagtgg taaacgctgc tggctggcgg tccgcaatgc gtcgctctgt cagttcctgg
     5641 aaaccagttt gcagcgcagc ggcatcgtcg ttacaacata cgaagggcag gaaccgactc
     5701 ccgaagatgt gttaattact gacgaggtag tgagtaaaaa atggcagggt agagcggtag
     5761 tgaccttctg tcgtcgccat attggtattc cgctggagaa agcgccaggg gagtgggtac
     5821 acagtgtggc tgccccgcat gagctaccgg cattgttggc gcgtatttat ttgatcgaga
     5881 tggagagcga cgatcctgct aacgctctgc cgtcgacgga caaagcggtc agcgataatg
     5941 acgatatgat gattctggtc gtggatgatc atccgattaa ccgacgtctg ctggcagatc
     6001 agttgggatc gttgggctat caatgtaaaa ccgcgaatga tggtgtcgat gcgcttaatg
     6061 tacttagcaa gaatcatatt gatatcgtgc ttagcgacgt caacatgcca aatatggatg
     6121 gttaccgctt gacgcaacgc attcgtcagt tgggactgac gttgccggta atcggagtaa
     6181 ctgctaatgc gttggctgaa gagaagcagc ggtgtctgga gtccagtatg gacagctgcc
     6241 tgtcgaagcc ggtaacgctg gatgtgataa aacagacgct gacggtatat gccgagaggg
     6301 tcaggaaatc gcgggaatcg taggacggat aaggtgttta tgtcgcatct ggcattcagt
     6361 gcaaatgcca gatgcgacgc tgacgcgtct tatctggcct actttgatgt aatctgttga
     6421 aataatggga atcgtaggac gcataaggcg tttacgccgc atccggcaat cggtgcaaat
     6481 gccagataag acactaacgc gtcttatctg gcctacaggt gattagtctt tatctgccgg
     6541 acttaacgtc actgaagaga gataattcag cagggcgata tcgttctcga cacccagctt
     6601 catcatcgca gatttcttct ggctactgat ggttttaata ctgcggttca gctttttagc
     6661 gatctcggtc accaggaagc cttccgcaaa caggcgcaga acttcactct cttttggcga
     6721 gagacgcttg tcaccgtaac caccagcact gattttttcc aacaggcgag aaacgctttc
     6781 cggggtaaat tttttccctt tctgcagcgc ggcgagagct ttcggcagat cggtcggtgc
     6841 accttgtttc agcacgatcc cttcgatatc cagatccaat accgcactaa gaatcgccgg
     6901 gttgttgttc atggtcagaa caatgattga caggcttggg aaatggcgct tgatgtactt
     6961 gattaaggta atgccatcgc cgtacttatc gccaggcatg gagagatcgg taatcaacac
     7021 atgcgcatcc agtttcggca ggttgttgat cagtgctgta gagtcttcaa attcgccgac
     7081 aacattcacc cactcaattt gctcaagtga tttgcgaata ccgaacaaga ctatcggatg
     7141 gtcatcggca ataattacgt tcatattgtt catgtaatag gctaccttgc tacagcaagc
     7201 tcttgacata actgtcaatg tcgctgatgt atttttctat tcctggaaca tccttctcac
     7261 gaatcagatg ttccagcgtt tcacataact gcttgccggg taccagattt agcatggcaa
     7321 atacgccttt aagacgatga gccgtttggg ctaacgcagc aaagtcactg gttgctgctt
     7381 cagtatacag cctcttaaca tcatccggta ctgtgtctac aaagagcgca taatagccgc
     7441 tggcatggag ttgcgcattt tcatctccgc ccagaggcga ttctgtcacc tcttcctgcg
     7501 ccagttgcac ttcaattaat tgtaagaccg cttcctgcat agcgttgctc atattgaagt
     7561 tgacgcacaa ttgaccaggc ccaatttccc gtacgccaga ctcatcatcg cttaaaagca
     7621 agccagaggc agtaagatta gacggattat ccgttaaaaa gatatcataa tcttgactaa
     7681 ttaatctttc atcgggtgtg atacaggttg caccccaatt ttctaactgg cgagtgacaa
     7741 tattccgaat ttctgccgaa gtaacatcca ccattacgca gacatcatcc agtaaacgct
     7801 cttcttcctc ttcaacttcc gggtcagcgg cgagcatttt gatatgcaca gagtagcgtg
     7861 taccaagccc atcccgcgtt ttgatgttta aatgaccgcc cagtttacgt gccagttgat
     7921 cgctcagcca gaatgccagc gggtccgcct tgccatagcg atcgttttgg gtctggttga
     7981 taaacgggaa gtgcaaatta tccatttcat gaatgcttac gccttctccc gtgtccagaa
     8041 tgcggaatgt caggcggtct tcggaggact catcctgatc aacctcaagg gtgatttttc
     8101 ccaattgcgt tgaggtcacg gcatattgca tcagtagtag caaaatacgt cgtaaggcat
     8161 cgcgatcgcc gcggcgcata tcgtgtgctt tcagatgatt gttaatcagc agttgcagac
     8221 ctttacgctt gatggcaggc aacactgaag gcacaacttc atcaattaaa tcctgcacgg
     8281 agaacagcac cgtctcactt ttccagctat cgtccgcaag catgttcgct aactgtattt
     8341 catcgaccag ccgcaccagc acatctgcct gattcgccag ttgtttgctt tccggggcat
     8401 tgagtttagc cgcgctctcc gccagggact gtgcgggttc tttcagcgca tcgccaatgt
     8461 ttttcataaa ggtcatccgc ccctgctggt ttttctcata cagacgctgc gcctgcttga
     8521 gtttcttgtt taccagcact tcgcgatcct gatcgcgaat aatgaaaatt tgtgtgcgcg
     8581 gcgcgacctg gctgcggaac atgcggatct catacagctc gttattgatc gtcgcctgaa
     8641 taatcccctg atgctgttcc gccatggtgg tgatgttttg cagattcaaa tgcggcagca
     8701 aatgatcggc aattttgtta cttatgacag tgcggttcga ttcctgatcg tgaaccagca
     8761 ggccgagcgg cagcagtgag actatctctt cattaattgc ccgtaaaatg cgcaattcgt
     8821 tattgaccgc cgtgctgggt agactttctg tactgcggct ggagaaatgg cggaatgtgg
     8881 tatagccaaa taacgccagc gccagcaaac cgatgttcag cagcagtggc agcagaatgt
     8941 tttgcaacgt atccagcaat aaggtgccat aaggaacctg ccagaccagg cgcatatcgg
     9001 tagagttgag tgccgaggag atttcaatct tcgtactgtt aaagtggata ctgacgctat
     9061 ccgtcccttc tttctcatta tcattgtctc ccgttgccgt cgcgtctggc tcaaggcgga
     9121 aactgtccag cggcataccc ggtgggatca aatcattaat cggcagatca aaagctacga
     9181 ccgttgccag atgtcccggc tggttgaacg tggtacgcaa ggtaaagtaa tgaccgttct
     9241 gccaggccag gcggcgcatg ttagaaaaac tttcgcgttc atcgagggcg ttggcctgtt
     9301 gcaacatctc tgcacgacgt gaatcaacaa tgtcactgac ggtcgattct ttaaatccgg
     9361 aggtgagatc tttgaggggc agggttgaga tcagcaccag actgttatcc tgaccattca
     9421 ggtaatacat tgaccacggt acattttctg caccccacaa tgtatccaga taagtggaca
     9481 tccgctgagt catctcaagc gttgagttgt cgtgagagcc aaagattaac gcttccgttt
     9541 tgcggcgtgg tttctccaga tagtagacat cctgtttcag gcgcgtctct tgtaaacctt
     9601 cgccggagga gggggaggtc gtcgcggcaa tgttgtcgta gatctgccag gtcacgtaac
     9661 gccaggtatc gacgcgcttt tggattgcgt gggtaatgtc gacaatctgg taacttttat
     9721 ctttcagcca tgcgttaacg gcgctttgta ccattatccc catcgtcacc agtaacacaa
     9781 tgatcaacag taaaaagaag cgggtaatgc tccccggtag gagtgaaaag cgggtcgtgg
     9841 ccgttgtctc tttctgacgc attcgtgttt atgacctgtt aaaacttcgc gaagtgatag
     9901 ggcagtataa agggtacagt gtgaaattcc agactcttac ctgattgatg attctctatt
     9961 ctcccgtgat atttatcatc ctgtatgggg gaaatagatg tacaagcgcc attttgctgt
    10021 acaaataacc tacaaaaagc ccaacgaaat taattgcaaa taaacgaaga ttgctggaaa
    10081 ttatgctgat gttaattatt tgtgagatag ttaacaagcg ttatagtttt tctgtggtag
    10141 cacagaataa taaaaagtgt gtaaagaagg gtaaaaaaaa ccggatgcga ggcatccggt
    10201 tgaaataggg gtaaacagac attcagaaat gaatgacggt aataaataaa gttaatgatg
    10261 atagcgggag ttattctagt tgcgagtgaa ggttttgttt tgacattcag tgctgtcaaa
    10321 tacttaagaa taagttattg attttaacct tgaattatta ttgcttgatg ttaggtgctt
    10381 atttcgccat tccgcaataa tcttaaaaag ttcccttcta tttacatttt gaaacatcta
    10441 tagcgataaa tgaaacatct taaaagtttt agtatcatat tcgtgttgga ttattctgca
    10501 tttttgggga gaatggactt gccgactgat taatgagggt taatcagtat gcagtggcat
    10561 aaaaaagcaa ataaaggcat ataacagagg gttaataaca tgaaagttaa agtactgtcc
    10621 ctcctggtcc cagctctgct ggtagcaggc gcagcaaacg ctgctgaagt ttacaacaaa
    10681 gacggcaaca aattagatct gtacggtaaa gtagacggcc tgcactattt ctctgacgac
    10741 aagtctgtag atggcgacca gacctacatg cgtcttggct tcaaaggtga aactcaggtt
    10801 actgaccagc tgaccggtta cggccagtgg gaatatcaga tccagggcaa cagcgctgaa
    10861 aacgaaaaca actcctggac ccgtgtggca ttcgccggtc tgaaattcca ggatgtaggt
    10921 tctttcgact acggtcgtaa ctacggcgtt gtttacgacg taacttcctg gaccgacgta
    10981 ctgccagaat tcggtggcga cacttacggt tctgacaact tcatgcagca gcgtggtaac
    11041 ggcttcgcga cctaccgtaa cactgacttc ttcggtctgg ttgacggcct gaactttgct
    11101 gttcagtacc agggtaaaaa cggtagcgta gatggcgaag gcatgaccaa caacggtcgt
    11161 ggtgctctgc gtcagaacgg cgacggcgtt ggcggttcta tcacttatga ttacgaaggc
    11221 ttcggtatcg gtgctgcagt ttccagctcc aaacgtactg atgatcaaaa tggtagctac
    11281 atcagcaatg gtgtagctcg taactacatc ggtactggcg accgtgctga aacctacact
    11341 ggtggtctga aatacgacgc taacaacatc tacctggctg ctcagtacac ccagacctac
    11401 aacgcaactc gcgtaggttc cctgggttgg gcgaacaaag cacagaactt cgaagctgtt
    11461 gctcagtacc agttcgactt cggtctgcgt ccgtctgtag catacctgca gtctaaaggt
    11521 aaaaacctgg gtgtcatcaa tggtcgtaac tacgacgacg aagatatcct gaaatatgtt
    11581 gatgttggcg cgacctacta cttcaacaaa aacatgtcca cctatgttga ctacaaaatc
    11641 aacctgctgg acgacaacca gttcactcgt gacgctggca tcaacactga taacatcgta
    11701 gctctgggtc tggtttacca gttctaatct cgattgatat cgaacaaagg gcctgcgggc
    11761 ccttttttca ttgttttcag cgtacaaact cagttttttg gtgtactctt gcgaccgttc
    11821 gcatgaggat aatcacgtat ggaaataagc tttacccgcg tggcactgct ggctgccgcg
    11881 ctcttctttg ttggttgcga tcaaaaacca caacccgcca aaacccacgc tactgaagtt
    11941 accgttcttg aaggcaaaac catgggtacc ttctggcgtg ccagcatccc gggcattgat
    12001 gccaaacgca gtgccgaact taaagaaaag attcagaccc agctggacgc cgacgatcag
    12061 ctgctttcga cctataaaaa agattccgcg ctgatgcgct ttaacgactc gcaaagtttg
    12121 tcgccgtggc cagtaagtga agcgatggcc gaaatcgtca ctacctcgct gcgcattggc
    12181 gcgaagaccg atggtgcgat ggatattacc gtcgggccgc tggtgaatct gtggggcttt
    12241 ggcccggaac aacagccggt tcaaattccg agccaggaac agatcgatgc gatgaaagcc
    12301 aaaaccggct tacagcacct gacggtcatc aatcagtcgc atcagcaata tctgcaaaaa
    12361 gacctgccgg atttatatat cgatctctcc accgtcggtg aaggttatgc ggcggatcac
    12421 ctggcacgct tgatggagca ggaagggatt tcccgctatc tggtgtcggt gggcggcgcg
    12481 ctgaacagcc gtggtatgaa cggtgaaggc cagccgtggc gggtagcgat tcaaaaacca
    12541 accgataaag aaaacgcggt tcaggctgtg gtggatatca acggtcatgg gattagcacc
    12601 tctggcagct accgtaacta ttacgaactg gacggcaaac gtctttccca tgttatcgat
    12661 ccgcaaaccg ggcgtcccat cgaacacaat ctggtatccg tgacggtgat tgctccgacg
    12721 gcgctggaag ccgatgcctg ggatactggc ttgatggtac tcgggccgga gaaagccaaa
    12781 gaggttgttc gccgggaagg gctggcggtt tatatgatca ccaaagaagg cgatagcttt
    12841 aaaacctgga tgtcaccaca gtttaaaagc ttccttgtca gcgaaaaaaa ttaaagcgca
    12901 agattgttgg tttttgcgtg atggtgaccg ggcagactaa aggctatcct taaccaggga
    12961 gctgattatg aaaaaagcca catgcttaac tgacgatcaa cgctggcaat ctgtcttagc
    13021 ccgcgacccg aatgccgacg gcgaattcgt tttcgccgtg cgtaccacag gcatcttttg
    13081 ccgtccgtct tgccgcgcca gacatgcttt gcgggaaaac gtctccttct acgcaaatgc
    13141 cagcgaggca ctcgccgctg gctttcgccc ctgcaaacgt tgtcagccag aaaaagccaa
    13201 tgcccagcaa catcggttgg ataaaatcac ccacgcgtgt cgactgctgg aacaggaaac
    13261 gcctgtaacg ctggaagcct tagccgacca ggtggcgatg agtccatttc atctacatcg
    13321 gttgtttaaa gcgactaccg gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg
    13381 ccgtttgcgc gaatcgctgg cgaaagggga gagcgtgacg acgtctattc ttaacgccgg
    13441 attccccgac agcagcagtt actatcgcaa agctgacgaa acgctgggca tgacggctaa
    13501 acaattccgt cacggtggcg aaaatctggc ggtgcgttac gcgctggctg attgtgagct
    13561 gggtcgttgc ctggtggcag aaagcgagcg ggggatttgc gcgatattgc tgggcgatga
    13621 tgacgcgaca ctaatcagcg agttgcagca gatgtttccc gctgccgaca acgcgcctgc
    13681 cgatctgatg tttcagcaac atgtgcgtga agtgatcgcc agcctcaatc aacgcgatac
    13741 gccgctgacg ttaccgctgg acattcgcgg cactgctttt cagcaacaag tctggcaggc
    13801 actgcgcacg ataccttgcg gtgaaaccgt cagttatcag caactggcta acgccatcgg
    13861 caaaccgaaa gcggtacggg ccgttgccag cgcctgtgcc gccaacaagc tggctatcat
    13921 aataccctgt catcgggtgg tccgtggtga tggcacactt tccggttacc gctggggcgt
    13981 gtcgcgtaaa gcgcaactgc tgcgccgcga agctgaaaat gaggagaggt aatgttggat
    14041 ctgtttgccg atgctgaacc gtggcaagag ccactggcgg ctggagcggt aattttgcac
    14101 cgttttgctt ttaacgctgc ggagcaactg atccgcgata ttaatgacgt tgccagccag
    14161 tcgccgtttc gccagatggt cacccccggg ggatatacca tgtcggtggc gatgaccaac
    14221 tgtgggcatc tgggctggac gagccatcgg caaggttatc tctattcgcc cattgatccg
    14281 caaacaaata aaccgtggcc cgccatgcca cagagttttc ataatttatg tcaacgtgcg
    14341 gctacggcgg cgggctatcc agatttccag ccagatgctt gtcttatcaa ccgctacgct
    14401 cctggcgcga aactgtcgct gcatcaggat aaagacgaac cggatctgcg cgcgccaatt
    14461 gtttctgttt ctctgggctt acccgcgatt tttcagtttg gtggcctgaa acgtaatgat
    14521 ccgctcaaac gtttgttgtt ggaacatggc gatgtggtga tatggggcgg tgaatcgcgg
    14581 ctgttttatc acggtattca accgttgaaa gcggggtttc atccactcac caccgactgc
    14641 cgctacaacc tgacattccg tcaggcaggt aaaaaagaat aaaaataaga attattattg
    14701 ctgtgcgcgc gaagatgttt aaactgcggg ctgtaattca ttgtccgggt tttctgcatg
    14761 gaacttcttg tacttgtctg gcggcagtat cgctggccat ttatcagtgt gatggcgcta
    14821 agcctcgcca gtgcggcatt aggcattggc ttaattgctt ttatcaatca gcgccttatc
    14881 gaaacggcgg ataccagtct gctggtattg ccggagtttc tgggattatt gctgctgttg
    14941 atggcagtca ctctcggatc gcaactggcg ctcaccactt tggggcatca cttcgtttac
    15001 cgactgcgta gtgaatttat caagcggatt ctggatacca acgtcgagcg cattgaacaa
    15061 ctcggtagcg cctcgttgct ggcgggatta accagcgatg tgcgcaatat caccattgct
    15121 tttgtgcgtc tgccggaact ggtgcagggg atcattctca ctatcggttc agcggcgtat
    15181 ctgtggatgc tgtcgggcaa aatgttgctg gtaacggcta tctggatggc gatcaccatc
    15241 tggggtggtt ttgtactggt ggcgcgggtg tacaaacata tggcgaccct gcgtgaaacc
    15301 gaagacaagc tgtacacgga ttttcaaacc gtactcgaag ggcgcaaaga gctgactctg
    15361 aatcgggaac gcgccgagta tgtgtttaac aacctctaca ttcctgatgc gcaagagtat
    15421 cgccatcata ttatccgcgc agacaccttc catcttagtg ccgtgaactg gtcaaacatc
    15481 atgatgctgg gcgctatcgg cctggtgttc tggatggcga acagtctcgg ttgggctgat
    15541 accaacgttg ctgcgaccta ttcgttgaca ctgttattcc tgcgtacgcc gctgctttcg
    15601 gcggttggcg cattgccgac gctgctgacg gcgcaggtgg cgtttaacaa gctgaacaaa
    15661 ttcgcactcg cgcctttcaa agcagagttt ccgcacccgc aggcgtttcc caactggcaa
    15721 acgctggagc tgcgtaacgt gacgtttgct tatcaggata acgcgttttc cgttggtccg
    15781 attaacctca ccatcaaacg tggtgaactg ctgtttctga ttggcggcaa cggtagcgga
    15841 aaatcgacgc tggcgatgtt gctgacgggc ttgtatcagc cacaaagcgg cgaaatcttg
    15901 ctggatggca aacctgtcag cggcgaacaa ccggaagatt atcgcaaact gttttcggca
    15961 gtgtttaccg atgtctggct gtttgatcaa ctgctggggc cggagggtaa acccgctaac
    16021 ccgcaactgg ttgagaagtg gctggcgcag ctgaaaatgg ctcataagct tgagttaagc
    16081 aacgggcgta ttgttaacct gaagttatca aaagggcaga aaaaacgcgt ggcgctgttg
    16141 ctggcgctgg cagaagaacg cgatattatc ctgctggatg aatgggcggc ggatcaggat
    16201 ccacacttcc gtcgtgagtt ttatcaggtg ttgctgccgc tgatgcagga gatgggtaaa
    16261 actattttcg ctatcagtca tgatgatcat tactttatcc acgccgaccg cctgctggaa
    16321 atgcgcaatg ggcaacttag cgagctgacg ggcgaagagc gcgatgccgc ttcgcgtgat
    16381 gccgttgccc ggacggcata atattacgac gctgacctgg cggactgctg ccgtcaggtc
    16441 aatatgctaa aaatcccttc atttctaata accctataat taatcaacga aattataatg
    16501 tttctaaaat tagaatataa tttataaaca ttatttaaat gttgttactt aagtgttacc
    16561 gttgatgccg cgcaaatctc acctgcaata aagcgactaa aagtaaggca ttaacaagat
    16621 gaaaaaagtg actgccatgc tcttctcgat ggccgtgggg cttaatgccg tttcgatggc
    16681 ggcaaaagcg aaagcgtccg aggagcagga aactgatgta ctgttgattg gcggcggcat
    16741 tatgagcgcc acgttgggga cctatttacg cgagctggag cctgaatggt cgatgaccat
    16801 ggtggagcgc ctggagggtg tcgcgcagga gagttcgaac ggctggaata acgccggaac
    16861 cgggcattct gcactgatgg aactgaacta caccccgcaa aacgccgatg gcagcatcag
    16921 tattgaaaaa gcagtcgcca ttaacgaagc atttcagatt tcccgccagt tctgggcgca
    16981 ccaggttgag cgcggcgtgc tgcgtactcc gcgttcattt atcaataccg ttccgcatat
    17041 gagctttgtc tggggcgagg ataacgttaa tttcctgcgc gcccgttacg ccgcgttgca
    17101 acaaagctcg ctgtttcgcg gtatgcgtta ctctgaagat cacgcgcaga tcaaagagtg
    17161 ggcaccgtta gtgatggaag ggcgcgatcc gcaacagaaa gtggcagcca cgcgtacgga
    17221 aattggtacc gatgtgaact acggcgagat cacccgccag ttaattgctt ccttgcagaa
    17281 gaaatctaac ttctcgctgc aactcagcag cgaagtccgc gccctaaagc gtaatgacga
    17341 taacacctgg accgttaccg ttgccgatct gaaaaacggc actgcacaga acatccgcgc
    17401 caaatttgtc tttatcggcg cgggcggtgc ggcgctgaag ctgttacagg aatcggggat
    17461 tccggaagcg aaagactacg ccggtttccc ggtgggcgga cagttccttg tttcggaaaa
    17521 tccagacgtg gttaatcacc atctggcgaa agtttacggt aaagcatccg ttggcgcacc
    17581 accgatgtcg gttccgcata tcgatacccg tgttctggac ggtaaacgcg tagtgctgtt
    17641 tgggccattt gccaccttct caaccaaatt cctcaaaaac ggttcattgt gggatctgat
    17701 gagttccacc accacctcta acgtgatgcc gatgatgcac gtcgggctgg ataatttcga
    17761 tctggtgaaa tatctggtga gtcaggtgat gttgagtgaa gaggatcgtt ttgaagcgtt
    17821 gaaagagtac tatccgcagg cgaaaaaaga ggactggcgt ttgtggcaag cggggcagcg
    17881 cgtgcagatt atcaagcgtg atgccgagaa aggtggcgta ctgcgtctgg gtactgaagt
    17941 ggtcagtgac cagcaaggga ccattgccgc gctgctgggg gcatcgccag gggcgtcaac
    18001 tgccgcgccg attatgttgg atctgctgga aaaagtattt ggcgatcgcg tttccagccc
    18061 gcaatggcag gctacgttga aagcgatcgt tccgtcttat ggacgcaagc tgaacggtga
    18121 tgtagcggca acagaacgcg agttgcagta caccagcgaa gtgctgggcc tgaaatacga
    18181 caagccgcag gcagcagata gtacgccgaa accgcagttg aaaccgcaac ccgttcaaaa
    18241 agaagtggcg gatattgcgt tgtaatgatg tgccacatcc ggcatggtat gccggatgtg
    18301 gcgtatgctg ataagacgcg ccagcgtcgc atcaggcaac cggctcgggc gttagatgtc
    18361 ggatgcggcg tgaccgcctt atccgaccta cggggtgacg cacttcacag cagtttagcg
    18421 aactaccgcg ttgtcaattt tctcttccgc cttccagacg cggtacttca catctacatt
    18481 gtctggcgta tacaccacga tcggcagctt gctgttgtaa cgcagcattc cagcatcgcc
    18541 cagatacgcg gtgacaaatt tcttctcttt cttgccatcc gggcaggcca tcatcgtgga
    18601 aaccggcgag ctaactttat caaagacata ataatcatag ccccagcctt ccagcgtttt
    18661 gttttccagc ttcccgctga gacgatgcaa attgcaatcg acttccagcg tctgaccgat
    18721 taacagttct actttcaggg tagattcatc ttcttgcggg gttaactgaa tcacctgacg
    18781 cttcatccct ttttcagctt gtggataagg cgcgattttt tccagtggct ggacgctttc
    18841 tgccgcccag gcggaagtgg tggcgaaagc ggcaaacaat actgcaggta gaatggtctt
    18901 catttttttg ctttccttct tcatttatag gttttcaccg atgatgtcgc tgaaggttaa
    18961 catattcatc agatttatta attt
//
LOCUS       DAJOSY010000083        18407 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJOSY010000083 DAJOSY010000000
VERSION     DAJOSY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.083"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(2..1459)
                     /gene="dtpC"
                     /locus_tag="OHN27_003117"
     CDS             complement(2..1459)
                     /gene="dtpC"
                     /locus_tag="OHN27_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCQ2287508.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(1518..3665)
                     /gene="cadA"
                     /locus_tag="OHN27_003118"
     CDS             complement(1518..3665)
                     /gene="cadA"
                     /locus_tag="OHN27_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCQ2287509.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(3745..5079)
                     /gene="cadB"
                     /locus_tag="OHN27_003119"
     CDS             complement(3745..5079)
                     /gene="cadB"
                     /locus_tag="OHN27_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCQ2287510.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(5444..6982)
                     /gene="cadC"
                     /locus_tag="OHN27_003120"
     CDS             complement(5444..6982)
                     /gene="cadC"
                     /locus_tag="OHN27_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HCQ2287511.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQKILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(7599..7674)
                     /locus_tag="OHN27_003121"
     tRNA            complement(7599..7674)
                     /locus_tag="OHN27_003121"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7639..7641),aa:Phe,seq:gaa)
     gene            complement(7781..8356)
                     /locus_tag="OHN27_003122"
     CDS             complement(7781..8356)
                     /locus_tag="OHN27_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCQ2287512.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(8393..10090)
                     /gene="dsbD"
                     /locus_tag="OHN27_003123"
     CDS             complement(8393..10090)
                     /gene="dsbD"
                     /locus_tag="OHN27_003123"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCQ2287513.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAAPQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRIVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(10066..10404)
                     /gene="cutA"
                     /locus_tag="OHN27_003124"
     CDS             complement(10066..10404)
                     /gene="cutA"
                     /locus_tag="OHN27_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCQ2287514.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(10520..11821)
                     /gene="dcuA"
                     /locus_tag="OHN27_003125"
     CDS             complement(10520..11821)
                     /gene="dcuA"
                     /locus_tag="OHN27_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCQ2287515.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(11939..13375)
                     /gene="aspA"
                     /locus_tag="OHN27_003126"
     CDS             complement(11939..13375)
                     /gene="aspA"
                     /locus_tag="OHN27_003126"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCQ2287516.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            13712..14188
                     /gene="fxsA"
                     /locus_tag="OHN27_003127"
     CDS             13712..14188
                     /gene="fxsA"
                     /locus_tag="OHN27_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCQ2287517.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(14204..15460)
                     /gene="yjeH"
                     /locus_tag="OHN27_003128"
     CDS             complement(14204..15460)
                     /gene="yjeH"
                     /locus_tag="OHN27_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCQ2287518.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            15736..16029
                     /locus_tag="OHN27_003129"
     CDS             15736..16029
                     /locus_tag="OHN27_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCQ2287519.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            16073..17719
                     /gene="groL"
                     /locus_tag="OHN27_003130"
     CDS             16073..17719
                     /gene="groL"
                     /locus_tag="OHN27_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCQ2287520.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            17857..18210
                     /locus_tag="OHN27_003131"
     CDS             17857..18210
                     /locus_tag="OHN27_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCQ2287521.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
BASE COUNT         4932 a         4455 c         4585 g         4435 t
ORIGIN      
        1 gttaatcgtt gctctcctgt atcatattcg tcggcgtgct aaacagaaaa cgggtagcaa
       61 aggccaatac gacgatgatc gcgacacaag ccaacgtcca ctctcccatc tgagaaaaga
      121 atcgctggta agctgcaatt gccataccgc taatttgcga ctctgtcgtc tgctgtgcca
      181 cgacgcctgc cagccagttg gcgaccgcgc ctgttgccag catataaatc ccggttaata
      241 cgccagacat tttcagacgc gtgatttgcg caatcgccac cgggtcaata aagagttcgg
      301 caaagcccat tagcgccagc ccggatatca tcacgcccat tgacgcttga ccgtcagccg
      361 ctgcatgtcg ggcatcaaat gccaacaaca taaagccaca agccatcagc agtaagccaa
      421 aggcaaactt cagccagacg cgcaatgttg agttgccgcg gctttctggc gacgccagcc
      481 aggccagtac aaccccagcg agcatcaccg caattgcatt caccgactgg aatagtgctg
      541 taggtacttc aatattgaat gcctgacgat tcacaaagcg atcgataaac aagctgatgg
      601 tactgccgcc ctgttgtgcc agtacccaga acaatgtccc gacaaacatc aacaatacaa
      661 tttgccaaag agcacggcga tgttcgggga atttgatcat catgcgggca atgatttgtg
      721 cggcaatgag gcaaacgatc gccagcaaat atccggacca gtcgttctcc agcagcagag
      781 taaaaaatac tggggctaaa cagagcatca ccactaacca gctccatact ggtaaggcaa
      841 atttgacact agtgagcgct tttttatcca tactacgtgt ggactggaaa tgacgatgac
      901 cgcttaagaa aatcaacaaa ccgataaaca tgccgccacc cgcaagggca aagccaacat
      961 gccatccata ccactgagca gccaggccgc aggcgattgg ggctgcgata gaaccgatat
     1021 tgcccgcagc atacagcagc gaaaaaccgc catcacgtcg atgatcgttc tcgtcgtaga
     1081 gctcgccaag caaacagctg atgtttgatt tgaataaacc gtagccacaa ataatgattg
     1141 ccagcgccag atacaggcta aaggttgaat ttgtatcaat acccagcacc acatggccaa
     1201 gggtcattaa cagcgcgccg gcaatcactg cagtgcggtt gccgagcagg cggtcggcaa
     1261 gccagccgcc gagaataggg gtaacgtaaa ccagagaagc atatgcgctg aacaggctga
     1321 tggcatggtt atcatcaaaa ccaagctgat gggtgagata gagaatgagt aaggcacgca
     1381 tgccgtaaaa actgaagtac tcccagattt ggatcgccac gatatagtat atcgcgcgcg
     1441 gctgtgaggg tgttttcatg tgttctcctt atgagcaaaa aagggaagtg gcaagccact
     1501 tcccttgtac gagctaatta ttttttgctt tcttctttca ataccttaac ggtatagcgg
     1561 ccatcagcct gacggtatgc accgtgaata tcggtttcaa agcccggata gtgagcgccg
     1621 atttcacaca gcatctgcag gaactccaga accggacggc tttcttcggt gatcatttca
     1681 cccggcatta ccagaggaac tcccggcgga tacggaagga tcatattggc gttaatacga
     1741 ccgaccattt cgtcgaggta aacttcttcg gtcataccgt gcagctcttt ctggaacgca
     1801 gcatacggag tcattaccat cgtcggcagc acttcaaatg cgcgatacat cagatccggc
     1861 agattgtggt gaacaatcag tttgtggata ttctgagcca gttcctgaat acgcatgttt
     1921 tcatagaatt caggatcttc acgatacaga gacggcagca tgtttttcac acgcaggttc
     1981 aggtcgaacg cacgtttgaa gtcagtcaga gcacgcagca ggctcagtgc tttggtctta
     2041 tcgataccga tgctgaacag gaacagcagg ttatacggac cggttttctc aacaacgatg
     2101 ccatgttcgt cgaggtattt cgccacgatg ctggccggaa taccaaagtc gctcatggtg
     2161 ccgtcttttt ccatccccgg agtcagcagg gtgactttga tcgggtcaag atacatgtgc
     2221 tcgttatcga tgtttttgaa gccgtgccag gtgctgtcag aacgcagcgg ccagcattca
     2281 gtggtatcga tatgatccgg ctgccataca tcaaagaacc agccatcaga ttccgttctc
     2341 agacgtttga tctctttacg gaatttgatc gcacgttcaa tggaaccgtt gatcagacgc
     2401 ttacctgcat tgcctttcat catcgccgca gcggtttcag tggacgccac gataccgtag
     2461 tgcggagaag tggtggtgtg catcatgtaa gcttcgttaa aggtttcttc gtttacgtca
     2521 cctttaacgt ggatcatgga agcctgagag aacgccgcca gcagtttgtg agtagactgg
     2581 gtttcgtaaa tcactttccc ttctacacgg ccaccgctca taccgcattt accttcgtaa
     2641 atcggtgaga agttggtgta aggtacccac gcggagtcaa agtggatgga tttcacatcc
     2701 agtgttttct tgatgaagtc ggtgttgtac agcagaccat cataggtaga gttagtaatt
     2761 acagcatgta ccggccaggt tgcgtttggt gtttctttca cgcgcttagc aatggtagcg
     2821 tgctggaatt cactctgtgg gataccacca agaataccgt aagcgttacg ggtcgggcgg
     2881 aaatagattg gcgtaacatc gctcatcatc atcaggtggg tcagcgattt gtggcagtta
     2941 cggtcaatca gaatggtgct gcctgctgga gcagagtaca taccaacaat tttgttcgca
     3001 gtggaagtac cgttggtcac catgtagctg cggtctgcgt taaagacgcg agcgatatac
     3061 tgttctgctt ctttgtgtgg accactgtga tccagcagag aacccagttc agatactgaa
     3121 atggaaatat cagatttcat ggtgttcgga ccaaagaaat catagaacag gctacctacc
     3181 gggcttttct ggaatgcagt accgcccatg tgaccaggag tacagaaagt atatttacct
     3241 tcacgaacat atttaaacag tgctttagtc agcggaggca gaatagtgtt gatatattcg
     3301 tcagtggtct gcttgatctt attagcaata tcttcagcag cacccagcgc atattcaaag
     3361 aagctaatct gtaaacgcag gtcattcagg cttacatcga gagtggaata cgtattagcg
     3421 aacgcgtaca acggcaggtt ctcgttcatt ttgctaattt cttcgcacag ctcgagatta
     3481 tatttatccc agtcaaaaat aacgccgcac agacgcgcat tgttttcgat cagttttaat
     3541 aagtcgtcac ggtcgttcgg gtaaacaatc tggaagttca gacgttcaag cgcgcgatga
     3601 agttcacgga tgggttcttc tttaaaataa acccccatgt gattcaatat tgcaataacg
     3661 ttcatagtca tatctccagg taaaaaaggc ccctcccaac acatgggaca aaatgaaagg
     3721 aggagcctcg gaaaatactt ttaattaatg tgcgttagac gcggtgtggt tatccattga
     3781 gtggctctgg cgctcgtgca ttttgcgagc gtagaacatc aggataatca ggctgacgat
     3841 gaaggtacct gccagctcga aggagcttgc gcccatcagc gcgatgaagc agaacacgca
     3901 acccagtaca gagcagatca ggctgacaaa gttgcggatg ttaacgcctt caaaacgaat
     3961 caggtcaacg caagagtaga aatacggcag catagtcagc agtactgcga taccggtcag
     4021 ttcaccgaac aggtcagatg ctttaccacc ggcagagttc atcagagtga taaggatcat
     4081 cagggcagtc attttcactg cagccagcag cagacctttt ttcggaatac cgttgctgtc
     4141 gacttcacca taaactttcg ggaagttacc gtcgttagcg gcacgtacac ctgcctggcc
     4201 taccaacatc atccaggagc ccagagaagt caggcacgca aaggcggtga atgcagaaac
     4261 cagcggcgca gcccagttac cgaggatagt tgaagcactg attgcaaacg gagcaccgga
     4321 agccgccatt acagaagacg gatacatacc ggaaagcacc tgagtcgcag cgatgtaaac
     4381 aatacctgct aaaccagtac ccagcatggt tgccagcgga acggtacgtt tcgggttttt
     4441 aaccatacca gtacttacag ctgcggattc aacacccacg aaggcccaca ggcagagcag
     4501 aatactttta atgatcgcat gaccatcagt ggtatccgca gtattccagt tagctgcata
     4561 agttgccgca tcaaaccaat gccagccaac aatagcagtc atcaccacag gaataagaac
     4621 cagcaccaga ccaatagtgg ttaaacggct tacccaagta ccgccgagca tatttacaaa
     4681 ggtaaatacc cagacgatag caatacaggc gatacccgcc ggaacaggat catttaatac
     4741 tgggaagaag gtggaaagat aagatacagc ggtaatacca atcgccaggt taccaatcca
     4801 gttagcatgg taataaagaa cacctgtctg aaaaccaaat gcaggggaaa tttctccggc
     4861 ataagcaatt gggccacctt gttgcgggtt ttttgttgcc agtcgggcat atacatacgc
     4921 cagcgacatt gcaccaataa tagagataat ccaaccccag atagcaatac caccgatact
     4981 tgctaggttc gcaggtaata atgcaatacc gctccccatc atattaccgg caacaacacc
     5041 ggtacaggca aatagcccga tcttcttggc agaactcatg ctcttctcct aatttcattt
     5101 ttgaatttgg agtccgggtc atgatgtata actatttcct gaccagacca aactggcgat
     5161 aagattactc acgaaaaaag gattaatcct aaagattagg tgaaataaca caaaagtttc
     5221 tgtaagtgag aacttgaggt tttttattaa cacatcagga tcgcaagttg atatcatgaa
     5281 aagataaaca tttaatgttt acaatggatt gcgtgacatt ctctggttaa atttatgtaa
     5341 taaaaattat gcggaaaata aattgccgca acatattata ccaacaggaa catacaaaaa
     5401 ctcaacaaca aatatttccg agcataaatc aacccggagt tacttattct gaagcaagaa
     5461 atttgtcgag ataaggtaca acataaggaa cagaagtctg gaatatacca ttttcaatcc
     5521 agtaaagggt gtttgcccct gggcgtaaat taaaggcggt gagatatgca tcagctgctt
     5581 cccggttcat ccccttcatt tcataaacct tgccaagcaa cacataattt agccaggaca
     5641 tttcaagatc aatgccagta tttatcgcct ggtaagactc atctgtttta ccttttacca
     5701 gagcactgac cgcttttatt tgatatataa tggacaggtt gttcaattcc ggcagtgtaa
     5761 caatgttatc tatttctgtg ttcagtgctg ctaattgttt ttcatctaaa ggatgttgag
     5821 aatggcgcac gatatcaact aatgcttttt ctgctctcgc gtaggtaaat tctggggatg
     5881 attgaacaat ctcacctaat aattcactgg cacggttcaa tgatttatca tcgccatgca
     5941 gtaaataatc atgtgcctga taaaaattag ttaataacgc accacgatgc ggcaaaattt
     6001 tctggagcgt ctcctgcatt cgttgtggcc acggttggtt taacgctttt gataaactct
     6061 ccagtaaatc attttgaatc gccagctgat taccgttagt gatgacataa cgtttatcca
     6121 gcatggttga accatctgca ttgtctacca attttatcga cataaagcat tgttgagcac
     6181 ggtattggcg ctgattaaca aacgcaatag ataatgtttt accggaactg ctcggttcat
     6241 caatgttgta gttgattttg tcatgcacca taaaggtgga gaaggtgtta agtgatgtcg
     6301 ccaccaaatc acccacgcct atcgcgtaag agagctgata cggggaactc cagctgttac
     6361 aacttttatt taccatatta atgtcaatat cgcgtggatt gagcaaaata cgcgatttgc
     6421 tcataggaag acgtgtatca agacttgaaa acgctaccag tgctacacag atacctaacg
     6481 acaacaggaa aaaaaaccat acccaaaagg tagtgaatcg tttgctttta actggggatt
     6541 gttcaggtgg cgttgcggtg ttttgaatgt taagactgtg ggagggagaa tctgtggcag
     6601 gaaccgcctc tggtataggg ggaggcgaag atagcattat ttcctctccc tcttcttcgc
     6661 tgtaccagat aaccggcacc attaatttat agccgcgctt tggtacagta gcgatataga
     6721 caggactatc ttcatcatta tcttttaatg acttacgtag ttctgagata ctctgcgtca
     6781 caacgtgatt ggtgacaata cttctcttcc agacattatc gataagttca tccctgctaa
     6841 gtacttcgcc actgtgttga gcaaagaaaa ccagaagatc gattaatctc ggctcaaggg
     6901 taagttgacg cccattgcgg ctaatttggt ttatggacgg agtaacaagc cattcgccaa
     6961 cgcgaactac aggttgttgc ataatagaaa ctcattcgaa aagggaatga tgcaatgata
     7021 attgccacaa cctattttta ccatctatag atgggtttat ttacatatta ttggtgaatg
     7081 caagacgtta tttttaccag ccataaactt cttgattaca tagtattacg aaaggatttt
     7141 actgagaacc agaagtaata ttccttacca tcaaaattca tcatctttgc caaagaaaaa
     7201 tgttcagaaa ataatccatg gaaaattgtc cggagcactt actattttaa tggattgtta
     7261 gtctttgcat gagcaagcga actgatacat ttctctttgt tctcattcag aaaatctcat
     7321 cagtcgccgt tcagaccggt gttgtgcttt atggatgcgt tacgctcctg atgacgtcat
     7381 ttgacgttca acagcatcac ggggccgcac gacatttcac gtcagttagt gctatagctc
     7441 aggaacaaat tttcccgaat tgggatatgc ccgcaaattg ctggtgatgt ggggagaatc
     7501 tggttgagtt cggtagaatt gatttggagg cagaacgctt aaatcgtggc gtcctgaaac
     7561 gaaaaacgga cctccgtgga ggtccgttta tatgaatttg gtgcccggac tcggaatcga
     7621 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     7681 gcgcattaaa ccgtaatctg cacatctcgt caacctaatt tcagtaaaag cgattcaact
     7741 gcttaagatt gcggcaaatc gcttcttttc tgtgtttctg tcaggtcaat gcaccaccct
     7801 ggcggcagcg ggcgaagcgc aggatatctt ccgccagccg atgtgcagtg tcgacatccg
     7861 cctggctacg attcaccagc attcggctta aacagccttc cagcaccagt tccatctgct
     7921 ttgctaccat cgccggatcg tcaacttcca gtgtggttaa cagttcgtgg gtgaaatcgt
     7981 aggccgcgct tttttgctga tcggccagtt gatgaatagg gtggccagga tcgggataaa
     8041 acgtacaggc agcgataaat agacagcccg gatagcggtt gtttttaacg cactccgata
     8101 acgcctgata acgtgccagc agcttttgtt cggcggtttg cgtttcgtcc agcatcagct
     8161 gacgacgcca gacatctatc tgttggctaa gataacgcag cgcatcgtag aggattgcct
     8221 ctttgtctgg ccagaagcgg cgtagctcgt ccagtggata atccacacgt tcagcaacca
     8281 tctccagcgt ggtgttggca atcccttgta attctaataa tttcagggct tctcccagta
     8341 catcttcacg ttgcacgcta ttttcctccg tctttcccac tgcaagtgtc gttcacggtt
     8401 ggcgatcgcg caaatgtgcg ctgaaggttt cagcatccat aaagcccgtg acgcgtgctt
     8461 gtggatgctc ctggccttgt ccgtcaaaaa agagaattgt cggtaggcca aggacattaa
     8521 gatgctttaa cagcgccaca tcttgtgcgt cgttggccgt gacgttggcc tgaagtaaga
     8581 ccgtgtctgc taacgctttt tgcacctgcg ggtcgctgaa ggtgtatttc tcaaactctt
     8641 tacaggcgac gcaccagtcg gcataaagat ctaacatcac cggtttgcct ttggcttcaa
     8701 cgagcgcctg atttaactca tctaccgttt tgatttgtgt aaagttgaga tgcgtctgag
     8761 tttgcgcggt atgcgtcgca ccaaatgccc aatcctgaag tgggcgcacg ctaaccaatg
     8821 ccgctgccag cagaataatt tgcacaatac gcatccagcc gcgtttagcc tgtaggctgg
     8881 tgataaaggc ccagccaaag aatgcgacac ccagcgccga ccacaagcgt aatccccata
     8941 catcaccaat cactcgctcc agcaggaaga ccggcagtgc gaggatcaca aaaccaaacg
     9001 cggttttgac ttgttccatc cacgggccgc ttttcggcag caagcggtta ccaaagacgg
     9061 taattagcat cagcggcagg cccatgccca acgcatagag ataaagcgtg ccgccgccca
     9121 gccacatgtt cccgctttgg gcgatataca gcagaatcgc gctaagcggt gcggtggtgc
     9181 atggtgaaca gatcagtccg gcaatcgccc ccataacaaa cacaccgcca ggtgagccgc
     9241 cctgttggcg attgctcatc aacgtgagac gtgtttgcag cgaagagggg agttgcaggg
     9301 taaacaagcc aaacattgac atcgccagca aggtaaagac gatggcgagg ccaatgagca
     9361 cgtatgggtg ctgtagcgcc gcctggaact gtaaccctgc ggcggcaacc accagaccca
     9421 gcgccgtgta ggtcagcgcc atcccctgca cataaataaa ggtcagcaac aatgctctgg
     9481 cagtggagag ccgctgttta ccacccagca cgatgccaga aatcagtggg tacattggca
     9541 gcacgcatgg cgtaaaggcg ataccaatac cgatcaacaa cgcccagagc gcggaaaagg
     9601 gcaattgcgc ggtgggctgc tcttgctgcg gaacagacac aggctgtggc gctgcgttgt
     9661 tggcgaccac ttcgcttaac ggaacggttt tggtttctgg cggataacag aaaccggcat
     9721 cagcacagcc ctggtaggtg acagttaacg tcgctcccgc actcgcctgg ttgatagtga
     9781 cgggaagcgt cagccgatcg cggtaaatct cgcttttgcc gtaaaactca tcttcatgcc
     9841 agacgccttg cggcagctgc acgtcggcaa ttttcgcgtg ttccggcgta atgcggatct
     9901 gtttacggta gaggtagtaa ccgtctttga tctgccaggt cagattaagg tcatgttggt
     9961 tttgctgaaa atcaaaagca aaggcttgat ccgcggggac aaattgtgaa cgtcccggcg
    10021 cgtcgaataa tccggcaaaa acggaagtgc tgcaaagtag caggatcagc gtaaagatgc
    10081 gttgagccat gagaggtaat ctgtgtctcc gtgtgtaaca ggtaaaacca gaagttccgg
    10141 ggtttgatat ggatgatgag acttcaggca ttccagcagt gcctgctggt gagatacggt
    10201 agtttttaaa atcatctgca cttcgtattc ttgctccagc ttaccttccc agtaatagag
    10261 agaggtagcg ccggggatca aggtcgcgca ggccgccagt ttttccgcca gcactttggc
    10321 ggctaaatcc tgggctgtcg cttcatctgg tgccgtacat agcaccacga cagacgcggt
    10381 attcgaactt ttttcatcaa gcataaacac ctcgcaagaa cagatggaac cgcaagagag
    10441 aggtcactat acaacggacg ggggaaggat gttagtcggc gggaaagaaa gcggggcgtg
    10501 aacgccccgc gattgatcat tacagcatga agctacccag cacgaagccg aagcaaacgg
    10561 ccagggcaac acccagagta cccgggatga agaacggatg gttgaagacg aatttaccga
    10621 tacgggtagt acccgtgtca tccatctgta ccgcagcaac cagcgtcggg taggtcggca
    10681 gaatgaacag accagacacc gcagcgaaag aagcaacagc ggtcagcggt gaaacgttca
    10741 gtgccagagc catcggcatc agtgcttttg cggttgcagc ctgagagtac agcagagcag
    10801 aagcaaagaa gaagatgacg gccagcagcc acggatgacc ctgaatcact tcaccagcgg
    10861 tatctttgat ccagtcgatg ttgttggaaa cgaaagtatc gcccagccac gcaacaccca
    10921 ggatacaaat acaggcgctc atacctgctt tgaaggtgct ggagttgagg atgttgtcgg
    10981 tatcgacttt acagataacg gtggtcagag ttgcaacgct gagcatgatg atcaggattg
    11041 cgttggtggt gttcatcagc ggtttttcaa ccagacccat gcttgggctg ttgatgattg
    11101 catagataac cacgccaact acgcccagca ggaacagcca gacggacgtt tttgcaccgg
    11161 atttgatttc aatctgcttt tcaccgcgca gttcaaccag gccctcttcc agacgcttgc
    11221 gataaatcgg atcgtcagag agtttggagt tgaacagcat agtgaccagg aaggacatca
    11281 ccagaaccgc cagcagggtg gacgggatga ccacggagag cagatggagg tagctgatgc
    11341 catgaccttc catcacggaa gacatgtaaa ccactgccgc tgagattggc gatgcggtga
    11401 tcgcaatctg cgcggatacc actgcagtag acagcggacg gcaaggttta acgccttgtt
    11461 ccttcgcaac ttcagcgata actggcagtg tcgccagaga gatgttgcca gtaccagcaa
    11521 agatagtcag gaaataggtc acgatcggtg cgaggatcgt gatgtatttc gggttacggc
    11581 gcagcagctt ttctgtctga tgaaccagat agtccagacc gccagcaacc tgcatggcag
    11641 aaatagcggc gataaccgcc atgataatgg agatgacatc gaacgggatg ttaccgggtt
    11701 taacgccaat agcggcaaga accagcaccc ccaatccgcc tgcaaaacca atacctattc
    11761 cccccaatct ggcgcccaag aagatcgcca gcaaaactat gatgagttct acaactagca
    11821 tattagcctt ccttgttttt taacaagttg atattagatt gttattttta agttactgct
    11881 cacaagaaaa aaggcacgtc atctgacgtg ccttttttat ttgtactacc ctgtacgatt
    11941 actgttcgct ttcatcagta tagcgttttg ctttgtaagc cgggtgcatc agattctgta
    12001 cggagaaaat atcgtcaagt tccgcttcag tcaacagacc gcgttccaga acgacttcac
    12061 gtacactctt accggtttcg gcacagattt tacccacgat gtcaccgttg tggtgaccga
    12121 tgaacgggtt caggtaagta acgataccga tagagttgta aacgtaacct tcgcacactt
    12181 ctttgttagc agtgatgccg ttaatgcatt tttccagcag gttgtagcaa gcgttggtca
    12241 gaatgtgaac ggattcgaac atggcctggc caatgaccgg ctccataacg ttcaactgca
    12301 gctgacctgc ttctgctgcc atggtaacag tggtgtcgtt accgatgact ttgaagcata
    12361 cctggttaac cacttccgga acaaccgggt ttactttagc tggcatgatg gaagagcccg
    12421 cctgcagttc cggcaggttg atctcgttca ggccggcacg tgggcctgaa gagagcaagc
    12481 gcaggtcgtt acagattttg gacatcttca cagccaggcg tttcagcgcg ccgtgaacca
    12541 taacataagc gccgcagtca gaggtcgctt cgatcaggtc ttcagccggt acgcatggga
    12601 agccagtaac ttcagccagt tttttcactg ccagcggaga gtactctttc ggcgtgttca
    12661 gaccagtacc gattgctgtt gcaccaaggt taacttccag cagcagttca gcggtacgtt
    12721 ggatgttttt cacttcttct ttcagcagga tgctgaaagc gcggaattcc tgaccgaggg
    12781 tcatcggtac tgcgtcctgc agctgggtac gacccatttt caggatgtcc tggaattcga
    12841 cagctttacg ttcaaagcct tcacgcagtt ggttaatcgc atctaccagc ttaatcaggg
    12901 aagagtaaac tgcgatacgg aaaccggtcg ggtaggcgtc gttagtggac tgacatttgt
    12961 taacatggtc gttcgggttc aggtactgat attcaccttt ttggtgaccc atcagttcca
    13021 gaccgatatt ggccagcact tcgttggtgt tcatgtttac ggaagtacct gcgccgccct
    13081 ggtagacgtc taccgggaac tgatccatgc attttccgtt gttcaggact tcatcacatg
    13141 cggcaatgat ggcattcgct acacttttag gaatggtttg cagctctttg tttgccatag
    13201 ctgcggcttt tttaaccatt accataccgc gaacaaattc aggaatatca ctgattttgt
    13261 tgttgctgat atagaagttt tcaatcgctc tcagagtgtg aacaccatag taggcatcag
    13321 ctggaacttc cctggtaccc aacagatctt cttcgatacg aatgttgttt gacatgtgaa
    13381 ccttcttttt caagctgcca atgatttgct ttaaacacac agaatatatg tggtttcgaa
    13441 tgtttttcga ccgacgatta tcccctgcat cgaccgaata cccgagatca tatgctgctt
    13501 gaggatttct accgtaatct ggatcacttt aagtgtcggt ttttacccct taattattaa
    13561 tttgtgaaat agatcaccgc tttgggatta ctaccaaaaa tagttgcgca aacatcttga
    13621 aattttgcta atgaccacaa tataagctaa acgcgattcg caacccattc aggtagccgg
    13681 ggttaaccgg ctgctattac aggagaaacc tttgcgctgg ttacctttta ttgccatttt
    13741 cctttatgtc tatattgaga tttcaatctt tattcaggtt gcccatgtat tgggggtatt
    13801 gctgaccctc gtgctggtta tattcacgtc agttatcggt atgtcactgg tacgtaacca
    13861 gggctttaag aatttcgtgc tgatgcagca aaaaatggcg gcgggtgaaa acccagcggc
    13921 ggagatgatt aaaagtgttt cgctgatcat tgctggtttg ctgcttttat taccgggctt
    13981 ttttaccgac ttcctcggtc ttctactttt attgccgccg gtgcaaaagc atctgacagt
    14041 gaagttgatg ccgcatttgc gcttttctcg catgcctggc ggcggtttta gcgccgggac
    14101 cggtggcggt aatacttttg atggtgagta ccagcgaaag gatgatgagc gcgaccgcct
    14161 tgatcataaa gacgatcgcc aggattaatg tcgaaacgcc ggattatgtg gttatgccat
    14221 tttccggcgt ttttcgtttt ggcagcaaca gccataaccc cgccagcatg atcagcgcat
    14281 agagactttt ccagccgacc attgccagta acagaacgca taacagcccg ccaaccaccg
    14341 ccagtagtcg ataacgtcct tgcaataatt tacagcctgc cagcatgcat aacagataaa
    14401 tcataataaa gatgccattg gcataaataa taagagcgtc cagattgatc tctaaagcat
    14461 gaatcaccaa agtgctcacc acacagcagc cgagcaccgc attgagggca ttattcggga
    14521 tatggcgaga agagaggcgt gccaggtagt ggtcaggatt atgttgcgcc tgcgaccaga
    14581 ccaggcgggc gaagctctgt atataaatgt tgagactggc aaagcaggcc agatagccaa
    14641 tcacgcaggc aatccataac gctcctacac cgaacaactg cactacaatt tttggaagcg
    14701 atgctgccgc cgccattttt tcaccatagg cgtcgaagtg taagacgact accgtacagc
    14761 cccagtagac taatcctgcc agcagcagac caatcatcaa agcacgagga aaatcacgct
    14821 ctggattttt aaattccgag gcgagatggg caaatgcctc cagaccgaca aaacaccaga
    14881 acatcactga taacgcagca aataacccgg taagttcgat attaccaggt gccggaaagg
    14941 ggatattcgc aggtttgata tcgcccgccc accagatagc gacaatcagc gcgacgataa
    15001 gtccggcaat aacggtttgt agattagcac tggaactggc accgcgagta ccgatatacc
    15061 acaccagcgc cagcgtaccg agttctgcca acaacagttg ccagctatgc cagccaaaca
    15121 tcgcctggcc gaacccggcg gcaatttgta gtgcggcagg caaacccacg ggaatgaccg
    15181 ataaaaacag ccagccggtg actcgctcaa gccgcgaacc aaacgccata ccgacgaagt
    15241 gcgcgacgcc gcctgcgctg ggatagtggc gacccagaat cgcaaacaca atcgcaatcg
    15301 ggaacactaa gataatcaaa acgggccacg cccacaggct gttattgccc gctaccagcg
    15361 cagctaacgc aggaacggca aacacgccag tgcctaataa tgacgtcgat agcaggccaa
    15421 tgccctgggc cagccccagt tcttgtttga gtccactcat gggttgatgt ccgattgcgc
    15481 ccaaattttg ggcaactgcg tagattttcg atggtagcac aatcagattc gcttatgacg
    15541 gcgatgaaga aattgcgatg aaatgtgagg tgaatcaggg ttttcacccg attttgtgct
    15601 gatcagaatt ttttttcttt ttcccccttg aaggggcgaa gcctcatccc catttctctg
    15661 gtcaccagcc gggaaaccac gtaagctccg gcgtcaccca taacagatac ggactttctc
    15721 aaaggagagt tatcaatgaa tattcgtcca ttgcatgatc gcgtgatcgt caagcgtaaa
    15781 gaagttgaaa ctaaatctgc tggcggcatc gttctgaccg gctctgcagc ggctaaatcc
    15841 acccgcggcg aagtgctggc tgtcggcaat ggccgtatcc ttgaaaatgg cgaagtgaag
    15901 ccgctggatg tgaaagttgg cgacatcgtt attttcaacg atggctacgg tgtgaaatct
    15961 gagaagatcg acaatgaaga agtgttgatc atgtccgaaa gcgacattct ggcaattgtt
    16021 gaagcgtaat cctcgcacga cactgaacat acgaatttaa ggaataaaga taatggcagc
    16081 taaagacgta aaattcggta acgacgctcg tgtgaaaatg ctgcgcggcg taaacgtact
    16141 ggcagatgca gtgaaagtta ccctcggtcc gaaaggccgt aacgtagttc tggataaatc
    16201 tttcggtgca ccgaccatca ccaaagatgg tgtttccgtt gctcgtgaaa tcgaactgga
    16261 agacaagttc gaaaacatgg gtgcgcagat ggtgaaagaa gttgcctcta aagcgaacga
    16321 cgctgcaggc gacggtacca ccactgcaac tgtactggct caggctatca tcactgaggg
    16381 tctgaaagct gttgctgcgg gcatgaaccc gatggacctg aaacgtggta tcgacaaagc
    16441 cgttaccgct gcagttgaag aactgaaagc gctgtccgta ccgtgctctg actctaaagc
    16501 gattgctcag gttggtacca tctccgctaa ctccgacgaa accgtaggta aactgatcgc
    16561 tgaagcgatg gacaaagtcg gtaaagaagg cgttatcacc gttgaagacg gtaccggtct
    16621 gcaggacgaa ctggacgtgg ttgaaggtat gcagttcgac cgtggctacc tgtctcctta
    16681 cttcatcaac aagccggaaa ctggcgcagt agaactggaa agcccgttca tcctgctggc
    16741 tgacaagaaa atctccaaca tccgcgaaat gctgccggtt ctggaagccg ttgccaaagc
    16801 aggcaaaccg ctgctgatca tcgctgaaga tgtagaaggc gaagcgctgg caactctggt
    16861 tgttaacacc atgcgtggca tcgtgaaagt tgctgcagtt aaagctccgg gcttcggcga
    16921 tcgtcgtaaa gctatgctgc aggatatcgc aaccctgact ggcggtaccg tgatctctga
    16981 agagatcggt atggaactgg aaaaagcaac cctggaagac ctgggtcagg ctaaacgtgt
    17041 tgtgatcaac aaagacacca ccactatcat cgatggcgtg ggtgaagaag ctgcaatcca
    17101 gggccgtgtt gctcagatcc gtcagcagat tgaagaagca acttctgact acgaccgtga
    17161 aaaactgcag gaacgcgtag cgaaactggc aggcggcgtt gcagttatca aagtaggtgc
    17221 tgctaccgaa gttgaaatga aagagaaaaa agcacgcgtt gaagacgccc tgcacgcgac
    17281 ccgtgctgcg gtagaagaag gcgtggttgc tggtggtggt gttgcgctga tccgcgtagc
    17341 gtctaaactg gctgacctgc gtggtcagaa cgaagaccag aacgtgggta tcaaagttgc
    17401 actgcgtgca atggaagctc cgctgcgtca gatcgtcctg aactgcggcg aagaaccgtc
    17461 tgttgttgct aacaccgtta aaggcggcga cggcaactac ggttacaacg cagcaaccga
    17521 agaatacggc aacatgatcg acatgggtat cctggaccca accaaagtaa cccgttctgc
    17581 tctgcagtac gcggcttctg tggctggctt gatgatcacc accgagtgca tggttaccga
    17641 cctgccgaaa aatgatgcag ctgacttagg cgctgctggc ggtatgggcg gcatgggtgg
    17701 catgggcggc atgatgtaat tgccctgcac ctcgcagaaa taaacaaacc cccgggcaga
    17761 aatgtctggg ggtttttctt ttggtcatct ttctagtata agattcagac acggacgacg
    17821 cgagtggcgt ccagctcatt gattatgggg aataacatgc acgtaaaata cttagcaggg
    17881 attgtcggtg ccgcgctact gatggcgggt tgtagctcca gcaacgaatt gagtgctgcc
    17941 ggtcagagtg tacgcattgt ggacgagcag ccaggcgcag agtgccagct gattggtact
    18001 gcgacaggta agcaaagcaa ctggctttcc gggcaacacg gagaagaggg cggttctatg
    18061 cgcggcgcag caaacgatct gcgcaaccag gcggctgcaa tgggcggtaa cgtgatttat
    18121 ggcatcagca gcccgtcgca gggaatgttg tccagttttg tcccgacgga tagccagatt
    18181 atcggtcagg tttataagtg cccgaactga tgtgatggtg acaaacgtat aattgccaga
    18241 ggcgcttcgc ttatcaggcc tacataaggc attgcaactg attgaatgct tgagaatttg
    18301 taggccggat aaggcgttta cgccgcatcc ggcatgacca acgatcacgt tatcagtcat
    18361 ttcaggctgg atggctccag cctcgcatta gcaaagatga gattatt
//
LOCUS       DAJOSY010000084        18256 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJOSY010000084 DAJOSY010000000
VERSION     DAJOSY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.084"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..572
                     /locus_tag="OHN27_003132"
                     /pseudo
     CDS             1..572
                     /locus_tag="OHN27_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            675..1178
                     /locus_tag="OHN27_003133"
     CDS             675..1178
                     /locus_tag="OHN27_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCQ2287522.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1245..2702)
                     /gene="nuoN"
                     /locus_tag="OHN27_003134"
     CDS             complement(1245..2702)
                     /gene="nuoN"
                     /locus_tag="OHN27_003134"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCQ2287523.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2709..4238)
                     /gene="nuoM"
                     /locus_tag="OHN27_003135"
     CDS             complement(2709..4238)
                     /gene="nuoM"
                     /locus_tag="OHN27_003135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCQ2287524.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4402..6243)
                     /gene="nuoL"
                     /locus_tag="OHN27_003136"
     CDS             complement(4402..6243)
                     /gene="nuoL"
                     /locus_tag="OHN27_003136"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCQ2287525.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6240..6542)
                     /gene="nuoK"
                     /locus_tag="OHN27_003137"
     CDS             complement(6240..6542)
                     /gene="nuoK"
                     /locus_tag="OHN27_003137"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCQ2287526.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6539..7093)
                     /gene="nuoJ"
                     /locus_tag="OHN27_003138"
     CDS             complement(6539..7093)
                     /gene="nuoJ"
                     /locus_tag="OHN27_003138"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCQ2287527.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7105..7647)
                     /gene="nuoI"
                     /locus_tag="OHN27_003139"
     CDS             complement(7105..7647)
                     /gene="nuoI"
                     /locus_tag="OHN27_003139"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCQ2287528.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7662..8639)
                     /gene="nuoH"
                     /locus_tag="OHN27_003140"
     CDS             complement(7662..8639)
                     /gene="nuoH"
                     /locus_tag="OHN27_003140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCQ2287529.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8636..11362)
                     /gene="nuoG"
                     /locus_tag="OHN27_003141"
     CDS             complement(8636..11362)
                     /gene="nuoG"
                     /locus_tag="OHN27_003141"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCQ2287530.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDDTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHREQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKYPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11415..12752)
                     /gene="nuoF"
                     /locus_tag="OHN27_003142"
     CDS             complement(11415..12752)
                     /gene="nuoF"
                     /locus_tag="OHN27_003142"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCQ2287531.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12749..13249)
                     /gene="nuoE"
                     /locus_tag="OHN27_003143"
     CDS             complement(12749..13249)
                     /gene="nuoE"
                     /locus_tag="OHN27_003143"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCQ2287532.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNITPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13252..15054)
                     /gene="nuoC"
                     /locus_tag="OHN27_003144"
     CDS             complement(13252..15054)
                     /gene="nuoC"
                     /locus_tag="OHN27_003144"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCQ2287533.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15148..15810)
                     /gene="nuoB"
                     /locus_tag="OHN27_003145"
     CDS             complement(15148..15810)
                     /gene="nuoB"
                     /locus_tag="OHN27_003145"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCQ2287534.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15826..16269)
                     /gene="nuoA"
                     /locus_tag="OHN27_003146"
     CDS             complement(15826..16269)
                     /gene="nuoA"
                     /locus_tag="OHN27_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCQ2287535.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16900..17838)
                     /gene="lrhA"
                     /locus_tag="OHN27_003147"
     CDS             complement(16900..17838)
                     /gene="lrhA"
                     /locus_tag="OHN27_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCQ2287536.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4561 a         5083 c         4705 g         3907 t
ORIGIN      
        1 ccaaccctgg gtcactgcat tatcgccaac gccccgacac gtaagtcctg agcaattaga
       61 aaaaacagtt cgttatctta cacaaactgt gcatccacgt aatgccggca atattgataa
      121 tctgaatagg tccgcagagt atataaaaga gttctttgtc agtagcggtg ccagagttac
      181 cgcgcagggc gtccccgatt ggctattacg actctgcgcc tggtagccag gattaccctt
      241 acccgcaatg tcctggcttt atcccgatcg gggggacttt attgcctggt cggcagaata
      301 caggatatca acgccgttcg tcaggtaaaa acggcattgt tatcatcccg ggatttatct
      361 gtttattcta tgaatgcccc atggtttatt cccggtattg atttctctga ccacctgaat
      421 tattggcaac acgatattcc cgccgtaatg attactgaca ccgcttttta tcgtaataaa
      481 caataccact tgcccggtga tacagcagac agattgaatt atcagaaaat ggctcagatg
      541 gtgttataac cttgttacac aacagtaaat aataaaaatt tctcgaggcg ggtccagaaa
      601 cagcgacatg tacattttat tttccctcgt ctgttttaag atgcgaaaca taaaattcaa
      661 tggaggaaaa ataaatggga atgattggtt attttgcaga aattgactca gaaaagatta
      721 accaactgct tgagtctact gaaaaacctt tgatggataa tatacatgac acgctttcag
      781 gattacgccg actggatatt gataaacgct gggatttttt acactttggg ctgacaggca
      841 cctctgcttt tgatcccgcg aagaatgatc cgctgagtcg tgccgtgctt ggtgaacaca
      901 gcctcgaaga tggcatagat ggcttcctgg gattgacctg gaatcaggag ttagccgcga
      961 caatcgaccg actggaatca ctggatcgaa gtgagctgcg taaacagttt tccattaagc
     1021 gactcaatga aatggagatt tatccgggag tgaccttttc agaagagtta gaggggcaac
     1081 tctttgcttc gatcatgctt gatatggaaa agctgattag cgcgtatcga cgtatgctgc
     1141 gacaagggaa tcatgcgctg acggttattg ttgggtgatg gtgtcagggc accataaaaa
     1201 accgccgaac atgtcggcgg ctttctgact tacaaactga tagcttacat cagcggcatt
     1261 gctaaccgca caatgctaat cagcggttgt ggccatacac ccagcaccag taccaacagt
     1321 gcagagatca gcaccacgat accgcctgcg ctgtactgcc agtttgacgg tgcatcgcga
     1381 cccggttgtt ccggggcgtg caggtacagg ctcaccgcca cgcgcaggta gtagtaaagg
     1441 ccgattgccg aaccgacaac cacggcgccc accagccacc acaagtgtgc ctggacaccg
     1501 accgccagca cgtagaactt accaataaag cccagcgtca tcggaatacc ggccagagac
     1561 agcatcatca ccgtcatcac cgccgcgagg atcgggcgat gccagaacag accgcggtaa
     1621 gagaacaggg aatcagcatc cgggccacgg tacgggctgg acatcaggct gaccacgccg
     1681 aacgcgccga ggctgctgaa cagataaccg gccaggtaaa ctcctaccgc ttccatcgac
     1741 atctcgcccg tttgcagcgc aatcagcgcc accagcagat agccgaggtg agagatagat
     1801 gagtaaccga gcagacgttt gatattggtc tggctcagcg ccatcaggtt accgaagatg
     1861 atagaggcaa aggcgataat cgccagcact acccgaatcg cttcgctgtc acccaccggt
     1921 gcgtagagga acagacgcat caccacaccg aagatagcga ttttgctcgc cgtcgccagg
     1981 aaagtggaaa ccggcgcagg cgcgccctgg tatacgtctg gcgtccacag gtggaacggc
     2041 accagagaga gtttgaagcc aaggccaaca atcatcaggc cgaaacctgc cagcaacagc
     2101 ggctcgttga gcataccgtc gccgaggttt ttacccagcg cgacaaacga caggtcgcca
     2161 gactgcgcat acaccagcgc cataccgaac agcaggaaag aagacgctgc ggcagaaagg
     2221 atagtgtatt tgatactggc ttccagcgaa cgtttctggc ggaaagcgta accgaccagg
     2281 ccaaacagcg gcaaagagat cagttcgata ccgaggaaca gagacgccag atggttggca
     2341 ttcgccagca ggatcccgcc cagcgcggca attaacacca acaggtagaa ctcatccttg
     2401 ttgtcgttat agccttcaag ccacgggtag gcgaaagtac aggtggcgag gctcgccaac
     2461 aataccagcc cggtgtaaag catggcgaaa ccatcaacgc gcatcagcgg cgtaacgtcc
     2521 atagcgcccg cctggccaac aaaccagagc gaaaccagcg ccgcgttaag cccaataacc
     2581 gagagcgtag cgttgaggaa atgattgcgt cgccacgcaa tggagagcat cacaaccacc
     2641 accgtcaagc cgacgatcag caacggtagc agtgcgatca ggttttgtgg agttattgtc
     2701 atggcgattt acggccttgt agtagtaacg gaattaacaa accactgctg gatattgcca
     2761 atcgcggagt gcgaggtatc cagaatcggc tgcggataga agcccagcag taccagcagc
     2821 accaccagca acaggatcat aaacagctca cgcagcgaca tccctggcag ttcctggctg
     2881 gcaatctggc ttttcgcttt accgaagtaa gcgcgatgta acatcgccag cgaataaaca
     2941 gatgcaaaga ccagcccaaa ggtagagata acggtaatca ccgggacaac ctggaagctg
     3001 ccgaacagaa tcataaattc gccgacgaag ttaccggtgc caggcatccc aagcgttgcc
     3061 accgcaaaga acagcgacag tgctggcagc catttcatct tgctccacag accgcccatc
     3121 atgcgcatgt cgcgggtatg gatacgttca taaagctgac cacaaagaat aaacagaccc
     3181 gccgccgaca aaccgtgcgc aatcatctgg attaccgcgc cctggtaggc caactggctg
     3241 ccggtgtaga tagcaatcag cacgaagccc atgtgggaaa ccgaggtgta ggcgatcaga
     3301 cgtttgatat cggtctgggc gaaggccatc cacgcaccgt agaagatgcc gataacaccc
     3361 agccacatag caattggcgc gaactctgcc gacgcgttcg ggaacagcgg cagggagaaa
     3421 cgcagcaaac cgtaagcggc agttttcagc aagatccccg cgaggtcaac ggaaccggcg
     3481 gtcggagcct gggagtgcgc atccggcagc cagccatgca gcggaaccac cggcattttg
     3541 actgcgaagg cgatgaagaa gcccagcatt aacaggtatt ccacaccact ggacattggc
     3601 gtattcagca gctcttcata gttgaaggtc cagacgccgg tcgcattgta gtgaacaaaa
     3661 accagcgcca ggatggcaat caacatcacc aggccactcg cctgggtgta aatgaagaac
     3721 ttggttgccg ccgtgatacg cgttttaccg tcagaggctt tatgccccca cagtgcgatc
     3781 aggaagtaca tcggcaccag catcatttcc cagaagaaga agaacaggaa catgtcgatg
     3841 gcaaggaaca cgccgataac gccgcccagg atccacatca ggttgaggtg gaagaagccc
     3901 tgatattttt cgatctcttt ccacgaacat agtaccgcca gcacaccgag cagaccggtc
     3961 agcacgacca tcagcagcga cagcccgtca atggcgagat gaatagagat accaaaacgc
     4021 gggatccacg gcatgtcgaa ttcagactgc cactgcggaa ttccggcgga ttgcgtcagt
     4081 gaataaccgc cctgcaacca cagttgcagc gacagcgcca gcgtcaatcc catggtgatc
     4141 agcgcgatcc agcgtggcac cttgacgcca aagcgttcgg tctgccagca caggaagccg
     4201 ccaataaagg gaattaatat tagccagggt agtaacatgg cgatctttat tccttgtaaa
     4261 agtcccgtca ggaccggatt ttcaacgaat tctcacgaca gaaaattctc ttcggactgg
     4321 gggtagtgtc ggatgcaccg cgaaggcggc ttatccgacc tacgttctta tatgcccagg
     4381 ggcaatccca caatccttaa ctcaacgcag taccatcaac agtgccagca ccacgaccgc
     4441 accgatgctc atggatgcca cataccagcg cagatagccg ttctcactta acagcagacc
     4501 tttacctgca aagcgggaaa gaaccgccgg gatgttcatc attgagttca gcggatcgcg
     4561 tttcagcaac caggcaatac ccaggaacgg cctgacgaac actttgtcgt acagccagtc
     4621 aaagccccag gcgttgtacc accaggtact cagcaggcgg cccggcgcac tgttggcgat
     4681 ggaggtcacc agagtacgtt tacccagcca cagccaggct gccagcagaa tgccgacaat
     4741 cgcgaccacg ccagaggtaa tttccagggt caacatgctg ccgtgcgcca gttccgtcgt
     4801 ttgcggaagc acgccctgca gcggcggtac aatcagtgcg ccaacgaagg tggaaaggat
     4861 cagcagcaca atcagcggca ggctgtgagt tacccctttc acggcgtgag cgtgaatttg
     4921 ttcttttccg tggaagacga tgaaaatcat acggaaggtg tagagcgagg tcataaacgc
     4981 accgaccaga cctgccacca tcagattgat atgaccattc gccatcgcac ccgcgaggat
     5041 ctcatcctta ctgaagaagc ccgcagtgac cagcggcagt gccgacagtg ctgcgccgcc
     5101 caccaggaag cagagataaa ccagcggaat agatttacgc agaccgccca tcttgaagat
     5161 gttctgttcg tgatggcagg ccagaatgac ggaaccggat gccaggaaca gcagcgcttt
     5221 aaagaacgcg tgagtcatca agtggaaaat cgccgcatcc catgcctgca cgccaagcgc
     5281 gaggaacatg tagccaatct ggctcatggt agagtaagcg agaacacgtt tgatgtcggt
     5341 ctgtaccagc gcggcaaaac cggccagcag cagcgtaacc gccccgacaa tacccaccag
     5401 atgcagaact tccggcgtca tcaggaacag gccgtgggta cgggcgatca ggtagacacc
     5461 cgcggttacc atcgttgcgg cgtggatcag cgcggagaca ggcgtcgggc ccgccatcgc
     5521 gtcagcaagc catgtctgca acggcaactg cgcagattta ccgaccgcac cgcccagcag
     5581 catcagcgtc gcccacatca gcatgttatt gccgtcagca aagtgcgctg gtgccagttc
     5641 caccatttcg cggaagttca gggtgcccag ttcgttgtaa agaatgaaca gtgcgaaagc
     5701 gaggaaaacg tcacccacac gggtcacgac gaacgctttc attgccgctg cgccattctt
     5761 cggatcggtg taatagaaac cgatcagcag ataggagcac aggcccacgc cttcccagcc
     5821 gaggtacatc agcagcaggt tatcggcgag aaccagaact accatgctgg cgatgaacag
     5881 gttggtgtag gcgaagaagc gagagtagcc ctcttcaccg cgcatgtacc aggaggcgta
     5941 catgtgaata aggaaaccca caccagtgac caccgagagc atggtcagcg acaggccgtc
     6001 cagcaccagg ttaaaaccga tgttaaagtc gcctaccgac atccacgtcc acagcggctg
     6061 gctgtatgcc tgctcgccgt tagcgaagaa atcaacgccg ataaaggcgg ttaccagcgc
     6121 cgccaggccc acagagccaa cgccgacgat cgccgaaacg ttttcagacc agcgcccacg
     6181 ggagaacgcc agcaggacga agccaatcaa tggcaaaata atggttaagg caagcatgtt
     6241 catccgcgca tctcacttac tgaatcgatg ttcaggttct ggcgacgacg gtgaagttgc
     6301 agcagcagcg caaggccgat actcgcttct gccgccgcga ggctgatggc gagaatgtac
     6361 atcacctgac cgtcggtctg accccagtag cttccggcga ccacaaaggc cagcgcggag
     6421 gcgttaatca tgatttccag accaatcagc ataaacagca gattgcgacg gataaccaga
     6481 ccggttaagc caagaacgaa taagattgcc gcgaggatca gtccatgttg taaggggatc
     6541 atgcgtgctc ctccgttttt cttttcgcgc tgtcgtcttt acgattgctc agcacttcac
     6601 ccgcacgctc ttcacgaccg acgtggaagg ccacaaccag acccgcgagc agcagcatag
     6661 aagccagttc caccgccagt acgtaaggcc cgaacagcgt aatacccact gctttagcac
     6721 tgattggcgt accgtcgata ccctgatcat taacaccaag gatggcgtaa acaatcacca
     6781 ccagcatgat ggccgacaaa attgccggac caatccacac ctgcggtttc agccactggc
     6841 gttcctgttc gatttctgaa ccgcccaggt tgagcatcat caccacgaac acgaacagca
     6901 ccataatggc acccgcgtag acgataattt ccagcgcacc ggcgaagtaa gcgcccagtg
     6961 agaagaacac cccggaaatc gccagcagcg aaataatcag gtacagcagt gcgtgtaccg
     7021 gattggtatg ggtgatcact cgcaaggtcg caagtatggc tatcaggcca cagatataaa
     7081 aagcgaactc cattgcccct ctccttacgg taacaggctc ttgacgtcga taggcttggc
     7141 ttcgttctct gcttcgccct tatctttgcc gtcgattgcc atacctgcca tccggtagaa
     7201 gttatattcc gggtatttgc ccggaccgga gatcagcaga tcctcttttt cgtaaaccag
     7261 atcctggcgc ttgtattccc ccatttcgaa atccggggtt aactgaatcg ccgtggtcgg
     7321 acaggcttct tcgcacagac cacagaaaat gcagcgtgag aagttgatgc ggaaaaattc
     7381 cggataccag cgaccgtctt tggtttctgc tttttgcagc gagatacagc cgaccgggca
     7441 ggctaccgcg cagaggttac aggctacgca acgctcttcg ccgtccgggt cgcgggtcag
     7501 aacgatacga ccacgataac ggggcggcag atagaccggc tcttccgggt acattcgcgt
     7561 ttcgcgtttg gcgaacgcgt gcaggccgat catccagata ctacgaacct gggtgccgaa
     7621 acctactaac aattctttta aggtcatggt cttattgccc cttattgcgc ctgccagaga
     7681 atgacagccg ccgttaccag caagttgatc agcgtcagcg gcaggcagat tttccagccg
     7741 aaggacatta cctggtcata acgcggacgc ggtaacgacg cacgaatcaa aatgaacatc
     7801 atcataaaga acgcggtttt cagcgcgaac cagatgaatg gcggtaacaa cgggccttgc
     7861 cagccaccga agaacagcgt caccatcaat gcagagatgg tcacaatccc gatgtactca
     7921 cccacgaaga acagaccgaa cttcataccg gaatattcaa tgtggtaacc atccgccagt
     7981 tcctgctcgg cttccggctg gtcaaacggg tgacggtgac ataccgccac gcccgcgatg
     8041 gcaaaggtaa taaaaccaaa gaattgcggg ataacgttcc acacatgcgc ctggctgttg
     8101 acgatgtcgg tcatgttgaa tgaaccggcc tgcgccacca cgcccatcag ggaaagcccg
     8161 aggaacactt cgtagctcag ggtctgcgca gaagcacgca tcgcacccag caacgagtat
     8221 ttgttgttac ttgaccagcc cgcaaacagc accgcgtaaa ccgccagacc tgccatcatc
     8281 aggaagaaca aaatcccgat gttcaggtcg gcaaccaccc aacccggact gactggcaca
     8341 atcgcaaagg ccagcagcag cgaggtaaag gcaatcatcg gtgccagggt aaagatgacg
     8401 cgatccgaga atttcgggat ccagtcttct ttaaagaaca ttttgatcat gtccgcaacc
     8461 agctggagcg aaccgcccca gccaacacgg ttaggtccgt aacggttctg gaacagaccc
     8521 agcaggcgac gttcgccaaa gctcatgaat gccccgcagg tgacaaccac cagcaggatc
     8581 accaccgctt tgaggatggt cagcaggatc tcaatcagtt ccggtgatat ccaactcatt
     8641 gttgtgcctc cttgagatcc tcaagatgcg cgccagccag caccggagca ataccggaca
     8701 tacccatcgg caagcccacc tgccctgccg tcagcccttc agcgatttca accggcagcg
     8761 tgaccgtatt gccatcgtaa ctaaaggaga cgtgtgtacc tgcgttcaca cctaacttcg
     8821 cggcatccgc tgggttgagt ttgatgtacg gctgcggcat acggctctgg aagaccggag
     8881 cacgctgtga caattcatcg ctgccaaaca ggtggtaata cggcgcgata cgccatttcc
     8941 cgtcctgcgg ctggaagcgt gccggtacgc tggtgaagta atccagacca ttttcgctgg
     9001 tttcaaacag acgcacgccc ggatcgccaa agcgcagttt gccgcccact tcgtcctgga
     9061 atttgttcca cgcctgcggg gagttccagc ccggcgccca ggcaaacggc acttgcgaac
     9121 ggtgcgcagt cggctggttg ttaccttcca tcgagaaggt gaacatggtg tcaatatcct
     9181 gcggctgacg cggctcatga acgctgatat tggcgcgcat ggcggtacga ccgctgtaac
     9241 ggtgcggttc acgggccagt ttctgcccac gaatacggaa tgtcgcatcc ggcgcagcat
     9301 ctttgatacc tgccagttcc gggattttcg ccacaacagc gtcaattaca tggtcgagct
     9361 gcgtccagtc cacttcacgg ctcagcaagg tgctgtgcag cgagtgcagc cagcgccagc
     9421 tttccagcat gacagttttg ctgtcgtaat aagccggatc gtaaacctgg aagaaacgtt
     9481 gggcgcgacc ttcattatta attaccgtac cgtcgctttc agcaaagctg gcagcggaaa
     9541 gcaccagatg ggcgttttcc atgatcgctg tgcgttgatg atcaaccacc atcaccagcg
     9601 gcgctttagc cagcgcagca ttcacgcggg tagcagaagc gtgacgatgc agatcgtttt
     9661 ccaacaccac caccgcgtcg gcgcgtccgg tttccagttc ggttaacgct tcttcaagcg
     9721 aaccgccacc cataatgccc agccccatgc tgttgacgga acgggcaatc atggtgatac
     9781 caacgtcagc gccgcgacct ttcagggctt tcgcgacgtt agccgcagcc tgaatcacct
     9841 ctatgctacc ggcgttcgtc ccggagataa tcaacggttt cttcgcacct gccagtgcct
     9901 gcacgatgac gtcgattttg ttttgcagct caggttcgat accgtcaacc gctggtgcgg
     9961 agttatccag cgcatgggcg atggcaaaac ctaaacgcgc ctgatcttca accggtgcgc
    10021 ggtaagtcca cgccgcgata tcatccagac gagtgtcatc aacgttggta acaaacagcg
    10081 gatacttcgc acgttgaccg atgttgagga ttgccgcaat ctgccagtca gccactttct
    10141 gtgctgcagc catttcgcgc gctttacctt tcaccgcctg acgcactgcc agcgcgacgc
    10201 gcgcgccggt ctgggtaacg tcttcgccca gcaccagtac cgcatcgtaa gattcgattt
    10261 cgcgcagagc cggagtataa atgccgcctt cacgcagcac tttcagcgcc agttgcagac
    10321 gttcctgctc acggtgagcg ataccggtgt agaagttttc ttcgcccacc agttcacgca
    10381 gcgcaaagtt gctttccacg ctggcacgcg gagaaccaat accgatcact ttcttcgact
    10441 gacgcagaat atctgccgcg ccctgcattg cctgttcggc gttgagggta atgaaatcat
    10501 cgccacgacg ctgtactggt tgacgcggac gatccttcag gttgacgtaa ccgtaaccga
    10561 aacgaccacg gtcgcagagg aagtagtggt ttaccgtacc gttgtaacgg ttttcgatac
    10621 gacgcagttc gccgtaacgt tcaccggggc tgatgttaca gccgatggaa cattgctggc
    10681 agatgctcgg cgcaaactgc atatcccatt tacggttgta acgctcggag tgcgttttgt
    10741 cggtaaatac gccggtcggg caaatttcga ccaggttacc ggaaaattcg ctttccagcg
    10801 tgccgtcttc cgggcgaccg aagtagacgt tgtcgtgcgc accgtaaacg cccagatctg
    10861 taccgtcagc gtaatctttg tagtaacgca cacagcggta gcaggcgatg cagcggttca
    10921 tttcgtgaga gatgaatggc cccaaatcct gattacggtg ggtacgtttg gtgaaacggt
    10981 agcgacggaa gctgtgtccg gtcatcacag tcatatcctg aagatggcag ttaccgccct
    11041 cttcacatac cggacagtcg tgcgggtggt tggtcatcaa ccactcgacc acgctttcac
    11101 ggaactgttt cgcttcttcg tcgtcaatgg aaataaaggt gtcatcggaa gccggtgtca
    11161 tacaggacat caccaggcga ccacgcgtgt cttccgcgtt ttggtattgc ttcaccgcac
    11221 actggcggca agcaccgaca cttcccagcg ccggatgcca gcaaaagtaa ggaatatcaa
    11281 ggcccagaga cagacaagct tccagcaggt tgtccgctcc gttgacctcg tattctttgc
    11341 cgtctacatg aattgtagcc attagcatgc ttccagtttt ctcagtcaga gactgagcgt
    11401 taatcgaaat tcggttacca gcgctctttc agcaggttcg gctgaatccc attaatcaaa
    11461 tgggtattgc tgaacggctg tttgattccc gcctcaaatt cttcgcggaa atatttgatg
    11521 gcgctctgta acggctccac tgcaccaggt gcgtgggcac agaaagtttt acccgggcct
    11581 aagaatcgac acagttgctc aagtgtttcg atatcgcccg gctgaccttc accacgctcc
    11641 agcgcacgca gaattttcac gctccacggc agaccgtcgc ggcacggcgt acaccagccg
    11701 caggactcac gggcgaaaaa ctcttccagg ttacgcacca gcgacaccat gttgatctca
    11761 tggtcaaccg ccatcgccag cgccgtaccc agacggcttc ccgctttacc gatactttcg
    11821 aattccatcg gcaggtcaag gtgcgcttcg gtcaggaagt cggtgcccgc gccgcctggc
    11881 tgccaggctt tgaatttcag accgtcacgc ataccaccgg cgtaatcttc gaggatctcg
    11941 cgcgcggtag taccaaacgg cagttcccac agtcccggat ttttcacccg accggagaag
    12001 cccatcagct tggtgccagc atctttactt ttcgagatgt tctgatacca ctccacgccg
    12061 ttagcgagga tcgccggaac gttacacagg gtttcgacgt tgttgacaca ggtcggtttg
    12121 ccccatacgc cggaggttgc cgggaagggg ggtttcgagc gtgggttagc acgacgccct
    12181 tccagggagt tgattaacgc tgtttcttcc ccgcagatgt agcgccctgc cccggtatgg
    12241 acgaacagtt cgaagtcgaa acctgttccc ataatgtttt tgccaagcaa gcccgcttca
    12301 gtggcttcgg caatggcgcg gcgcagattt actgccgctt cgatatattc gccacgcagg
    12361 aagatgtagc cacggtaagc tttcagcgca aacgcggaga tgagcatacc ttccaccagc
    12421 aggtgcggca gttgctccat caacaggcgg tctttatagg tgcccggctc catttcatcg
    12481 gcgttacaca gcaggtaacg gatgttcatg gattcgtctt tcggcatcag gctccacttc
    12541 aggccagtcg aaaagcccgc tccgccgcgc cctttcagac cagcgtcttt tacctgatta
    12601 acgatttcgt ccggagacag cccggtcagc gccttacgcg ccccttcgta accgtttttg
    12661 ctgcggtatt cgtccagcca cactggctgt ttgtcatcgc gcagacgcca ggtcagcgga
    12721 tgcgtttcgg gagtacggat aatgtttttc atttataccg ctccagcagt tcagggatcg
    12781 cttccggggt cagatgcgcg tgagtgtcct catcgatcat catgtttggc cctttatcac
    12841 agttccccag gcagcaagtt ggcagcagcg taaagcggcc atcaaatgtc gtttgccctg
    12901 gtgtgatgtt cagctttttc tcgagcgccg cctgaatacc ctgatacccg ttgatatgac
    12961 agaccacgct gtcgcaataa cggatcacat ggcgaccaac cggctggcgg aagatctgac
    13021 tgtagaacgt tgccacacct tcgacgtcgc ttgccggaat acccagcaca tcggcgatcg
    13081 cgtggatcgc accatccggc acccagccac gctgcttctg aacgattttc agcgcttcaa
    13141 tggacgccgc acgcgggtct tcgtagtggt gcatctcgtg ctcaatcgct tcacgctctg
    13201 ccgcactcag ctcaaaagcc tcggtttgtg gttgttgatt ctcgtgcata attagcggtc
    13261 cacatctgac ataacaaaat cgatactgcc cagataaaca atcaggtcag acaccaggct
    13321 gccgcggatc gccgccggaa tttgctgcaa atgcgcataa ctcggcgtgc ggatacgagt
    13381 gcgataactc atggtgctac cgtcgctggt caggtagtaa ctgttgatcc ccttggtcgc
    13441 ctcaatcatc tggaaagatt cattggcagg catcaccggc ccccacgaca cttgcaggaa
    13501 gtgggtgatc agggtttcga tatgttgcag cgtgcgctct ttcggcggcg gcgtggtcag
    13561 cgggtgatcc gctttgaacg ggccttccgg catgttgttg aggcactgct caagaatgcg
    13621 cagactctgg cgcagctctt ccactttcag cattacgcgg gtgtagcagt cagaaacacc
    13681 accaccaacc gggatttcaa agtcgaagtt ttcatagcca gaataaggac gcgccttacg
    13741 cacgtcgaag tcgatcccgg tagcacgcag gcccgcgcca gtggtgcccc actccagcgc
    13801 ctctttcgcg ccataggcgg caacgccctg ggaacgacct ttcagaatgg tattttgcag
    13861 cgccgctttc tcgtaagacg ccagacgttt cggcatccag tcgaggaact cacgcagcag
    13921 gcgatcccag ccgcgcggca ggtcgtgcgc tacgccgcca atacggaacc acgccgggtg
    13981 catacggaaa cccgtgatcg cttccaccag atcgtaaatt ttctgacgat cggtaaaggc
    14041 gaagaacacg ggcgtcattg cgccgacgtc ctgaataaag gtcgagatgt acagcaggtg
    14101 gctgttgata cggaacagtt cggagagcat aacgcgaatg acgttaacgc gatccggcac
    14161 ggtgatcccg gccagtttct ctaccgccag cacgtaaggc atttcgttaa cgcagccgcc
    14221 gaggtattca atacggtcgg tatacggaat gtagctgtgc caggactggc gctcgcccat
    14281 tttctccgca ccacggtggt ggtagccgat gtctggtacg cagtcgacaa tctcttcgcc
    14341 gtcgagttgt aaaacgatac ggaaagcccc gtgcgccgac gggtggttcg gaccgaggtt
    14401 gaggaacatg aagtcctcgt tttcggtgcc gcgcttcatc ccccactctt ccggtttgaa
    14461 ggtcagggct tccatctcca gatcctgttt ggctttggtc agctcaaatg gcgagaattc
    14521 ggtagcgcgc gccgggtaat ctttacgcag cgggtgacct ttccaggttt gcggcatcat
    14581 gatgcggcgc aggttcgggt gaccgtcgaa agtaatgcca aacagatccc aggtttcacg
    14641 ctcataccag ttagcgttcg ggaacagttt ggtgaaggtc ggtacgtgca ggtcgttttc
    14701 tgccagcgcc accttcagca tgatgtcgcg gttacgatcg atagaaatca gatggtagaa
    14761 aacggaaaaa tccgcggcag gtaacccttc gcggtgtgtg cgcagacgtt cgtccatgcc
    14821 gtgtaagtca aacagcatga cgtaaggttt cggcagtttc tttaagaaat cgccaacttc
    14881 cagtaattgt tcacgcttga tccacacaac gggaaccccg gtgcgagtcg cctgaacagt
    14941 aaaggcatcc ggcccaaaac ggttgcgcag ttcgccaatt accggatcat caagatgatc
    15001 gcgggtctgc caggcgggtt cttgcgcggt taagtcggtc atattgttca ccattgcaaa
    15061 tggtccgtgg tgactgtcgg cgcagtaagc ttcgcgaaat agatttagga ataagcgaag
    15121 aaaaatccct ttgccgacag gcgcaaatta aatctcgtca ggtgtacgca ggttggttac
    15181 ggcaatgcgt tcaccgcgct tgcgttcgcg ctctgattgc atattggcgc gataaacgcc
    15241 ctgatcgcca accacccagg agagcggacg acgttctttg ccgatagatt cctgcaacag
    15301 catcagtgcc tgcatgtacg cttcaggacg cggcgggcag cccgggatat acacatcaac
    15361 cgggatgaat ttatcgacgc cctgcacaac ggaataaata tcgtacatac caccagagtt
    15421 ggcacaggca cccattgaga taacccattt tggttccagc atctggtcat acagacgctg
    15481 aataaccggt gccattttgg taaagcaggt tcctgcaacc accatcaggt cagcctgacg
    15541 cggcgaagca cgcaatactt ctgcgccaaa acgcgccacg tcatgcaccg cggtaaacga
    15601 agtcaccatc tcaacgtaac agcaggaaag accgaagtta tacggccaaa ttgagttttt
    15661 acgaccccag ttaaccatgt cattgagctt gcccataaac acgtttttgt taacttcttg
    15721 ctccagaggg tcggttacga tctcctgctt ttgcaggggg taacggtcgt tctcaccgtt
    15781 gggatctatg cgggtgagcg tataatccat cttaatgcct cgcggttagc gttgacgatt
    15841 agcgatactg ttcgtttccg ggttcatacg ctcgcggcgt gaacgcgcgg gcgtccagtc
    15901 cagcgcgcca atacgcacca gataaaccag acctgccagt aacacaaaaa taaaaattgc
    15961 agcttccaca aagcctaccc agccgctttc gcggatagag gttgaccatg cgaacagata
    16021 cagcgcttca acgtcgaaga taacgaagaa catggccacc agataaaact tggcggacag
    16081 gcgtaagcgg gcggagccga ccgagtcgat accggattca aacggcacgt ttttcgacct
    16141 cgcgcgtgcg cgaccgccta aaaaccaacc gcctaccagc atcaggcaac acaggccaat
    16201 ggcaacgata agaaagatag cgaatgccca gtgatgagcg atgacttcag tggatgttga
    16261 catactcatt gcttactcat caaaagtagc gccagattca ctgctcttca cggcagatgg
    16321 acgccacatc gattcatggg gaggaataaa aaaaacctta caatcactgt agaaattctt
    16381 ttatacagct aattgatgtg gtcttttact cctttctata accttttgtc aactttaaca
    16441 aaagtttctt cacattagtt tacataatat caacaccatt agcatttaat gccctttcac
    16501 cccagatcct tgacgactcc aggataatta gatgttgttg aatcgtgtcc gttgtgaagc
    16561 aatggaaaaa atacgggtct attttgacag gaatttgtgt cgattcctcc ccccaaaaga
    16621 gagtattttc ttgatctgtg acacgctttt gtcattccat aacaaaaacg cagcaacaaa
    16681 tttacgtatt ttttaacatc attgtagcag gtgatttttt tcaggcgatt atttgtgcgt
    16741 tcgggacgtg aatctctggt gattgaaaaa tgaacagttt tgtacgttct gcactatgcg
    16801 aggaaggatt tttactaaaa aaaagccgct ggggtttaaa acacccccag cggctcgttt
    16861 tttacactat tgtctcagga attatctatc gtccgtcgat tactcgatat ccctttcaat
    16921 caacaatgaa tcatcccctt ccggagcaga cattgggctg tattgccacg gattatggta
    16981 gctctccatc gcctgataga tcacctgggc cagctcatta ttactggacg ggtcatagca
    17041 cagcaaatat tcggtatcag gtagcggcgg taagccatct actccactca gcacacgcag
    17101 gtccgggctc atcatctcaa ccggccttgc cgtcacgcca agaccggctt tcactgccgc
    17161 acgaacggcc ggaagcgtcg aggcgacata agccagtcgc catggaatat ctgctttatt
    17221 aagcgtcgcc agcaccatat cgcgaaacgg acttggatca tccagcaata caagagggat
    17281 cggctcacct ttttgcaata cgtattccgc tgcgcagtac cagtgtgttg gcgaggtacg
    17341 caggttcaat gccttaaagg tagaaggacg atgggtggtt accatcaaat ccacttcctg
    17401 ggattccagc atttctgcca tataggcatt acgtttaacg cggacatcca gcgctaattt
    17461 cggataaacc gaactcacgc gatttaacag gaaaggtaag atcgtatcgg cagattcatc
    17521 tgaagcaccg atagttaaca cgccctgaag attactgaac attaatgatg agcaggcctc
    17581 atcattaaaa cgcaggattt tcctggcgta accaagaagt tgaatgccat gttcagttaa
    17641 cagtttgttg cgaccgtgac gagcgaacag ttctttccca acgagttgtt ccagacgctg
    17701 catttgctga cttacggcgg actgagtacg acacacagcg gcagctgcgg cagcaaaagt
    17761 gttcagatcg gcaacagcaa caaatgttct cagcagatcg aggtcgaggt taattatcgg
    17821 acgatttgca cttatcatat attatcactt actggcggct catactgagc tggttaatgc
    17881 tgtgcacaca caaacaagca attccatttg taatgtgcct ccctggcagt ttcatccgga
    17941 acccggacga aagtaaaaat gcatatgagt tgcactaaaa aagcgactca cattgttccg
    18001 ttataatgcc tgaagtagat cacagaatat atcttcaggg atcgcatatc tattaagtta
    18061 ctcactcttt tctatttatg acatgcgcgt gtttgtataa atgtaaatgt gagtccttgt
    18121 tccactctcg tgcagcatcg ctggtcatac gcgaacacgt acaaacagca atggtgaggc
    18181 ggcatcaaga gcagggatcc gtcatttatg cgagcatttt accccaaaaa ccttttattt
    18241 ataagggtca ttgcga
//
LOCUS       DAJOSY010000085        18091 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJOSY010000085 DAJOSY010000000
VERSION     DAJOSY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.085"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            47..586
                     /locus_tag="OHN27_003148"
     CDS             47..586
                     /locus_tag="OHN27_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HCQ2287537.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCLTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(996..2129)
                     /gene="thiH"
                     /locus_tag="OHN27_003149"
     CDS             complement(996..2129)
                     /gene="thiH"
                     /locus_tag="OHN27_003149"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCQ2287538.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVEWALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(2126..2896)
                     /gene="thiG"
                     /locus_tag="OHN27_003150"
     CDS             complement(2126..2896)
                     /gene="thiG"
                     /locus_tag="OHN27_003150"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCQ2287539.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQAGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(2898..3098)
                     /gene="thiS"
                     /locus_tag="OHN27_003151"
     CDS             complement(2898..3098)
                     /gene="thiS"
                     /locus_tag="OHN27_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCQ2287540.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(3082..3837)
                     /gene="thiF"
                     /locus_tag="OHN27_003152"
     CDS             complement(3082..3837)
                     /gene="thiF"
                     /locus_tag="OHN27_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCQ2287541.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LHQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(3830..4465)
                     /gene="thiE"
                     /locus_tag="OHN27_003153"
     CDS             complement(3830..4465)
                     /gene="thiE"
                     /locus_tag="OHN27_003153"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCQ2287542.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVMAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQTTDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(4465..6360)
                     /gene="thiC"
                     /locus_tag="OHN27_003154"
     CDS             complement(4465..6360)
                     /gene="thiC"
                     /locus_tag="OHN27_003154"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCQ2287543.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(6441..6541)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6594..7070)
                     /gene="rsd"
                     /locus_tag="OHN27_003155"
     CDS             complement(6594..7070)
                     /gene="rsd"
                     /locus_tag="OHN27_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCQ2287544.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            7165..7938
                     /gene="nudC"
                     /locus_tag="OHN27_003156"
     CDS             7165..7938
                     /gene="nudC"
                     /locus_tag="OHN27_003156"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCQ2287545.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            7978..9042
                     /gene="hemE"
                     /locus_tag="OHN27_003157"
     CDS             7978..9042
                     /gene="hemE"
                     /locus_tag="OHN27_003157"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCQ2287546.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            9052..9723
                     /gene="nfi"
                     /locus_tag="OHN27_003158"
     CDS             9052..9723
                     /gene="nfi"
                     /locus_tag="OHN27_003158"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCQ2287547.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAIVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            9766..10356
                     /locus_tag="OHN27_003159"
     CDS             9766..10356
                     /locus_tag="OHN27_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCQ2287548.1"
                     /translation="MLQNPIHLRLERLESWQYVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            10543..10815
                     /gene="hupA"
                     /locus_tag="OHN27_003160"
     CDS             10543..10815
                     /gene="hupA"
                     /locus_tag="OHN27_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCQ2287549.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            10828..11523
                     /locus_tag="OHN27_003161"
     CDS             10828..11523
                     /locus_tag="OHN27_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCQ2287550.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(11525..11944)
                     /gene="zraP"
                     /locus_tag="OHN27_003162"
     CDS             complement(11525..11944)
                     /gene="zraP"
                     /locus_tag="OHN27_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCQ2287551.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMGHW"
     gene            12182..13558
                     /gene="zraS"
                     /locus_tag="OHN27_003163"
     CDS             12182..13558
                     /gene="zraS"
                     /locus_tag="OHN27_003163"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCQ2287552.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPVIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAAGQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLCLPVN
                     ITRKDPQG"
     gene            13555..14880
                     /gene="zraR"
                     /locus_tag="OHN27_003164"
     CDS             13555..14880
                     /gene="zraR"
                     /locus_tag="OHN27_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCQ2287553.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(14877..16166)
                     /gene="purD"
                     /locus_tag="OHN27_003165"
     CDS             complement(14877..16166)
                     /gene="purD"
                     /locus_tag="OHN27_003165"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCQ2287554.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(16178..17767)
                     /gene="purH"
                     /locus_tag="OHN27_003166"
     CDS             complement(16178..17767)
                     /gene="purH"
                     /locus_tag="OHN27_003166"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCQ2287555.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKLDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         4091 a         4828 c         4962 g         4210 t
ORIGIN      
        1 gcaaggattc gatactattc ctgtgtaact ttcttaagga acgagaatga aacaggaagt
       61 ggaaaagtgg cgaccttttg gacatccgga tggtgatatt cgtgatttat catttcttga
      121 tgctcatcag gctgtctacg ttcagcatca tgagggcaaa gagcctttag agtatcgctt
      181 ttgggttacc tactctcttc actgcctcac aaaagattat gaacatcaga cgaacgaaga
      241 aaaacaatcg ttaatgtacc acgcgcctaa agaatctcgt cccttctgcc agcaccgtta
      301 taacttagcg cgcacacact taaaaagaac tattttggcg ctgccagaaa gcaacgttat
      361 tcatgccggg tatggtagct atgccgtgat tgaggtggat ttagacggag gagataaggc
      421 attttacttt gttgcgttca gggctttcag ggaaaagaaa aaactccgtt tgcatgtaac
      481 tagcgcttat cccatttctg aaaaacagaa aggtaaatca gtgaaatttt tcaccattgc
      541 ctacaactta ttgagaaata agcagcttcc tcagccctca aaataacaaa acccacctta
      601 aggtgggttt cgccagagaa ttatctctgg tattcagaac gccattaccg gactttgcct
      661 tgaccttgcg ataatcgcag gttgcgggat gtctgaattt cttcagtctg ctgcatcctg
      721 gaagatgaga acatgtgttc ttattttcgt ctctatcata gttgagtatt tactctctta
      781 caatcagatc tctttcattg ctcaacaggc gatggcttca gactttgcat tacggaattt
      841 ttaagaaagg cagggcgaaa cgaggaagaa gcttttcgcc ctggacaaca ttcctgctga
      901 cggcactacc ataaaaaaac gcaacgtttt caacaacctt gcgtttttca tcttccgtag
      961 cggctcgtaa taaatttacg ttcttacatt ctgtctcatg gcctttgcga ggcgcgtccc
     1021 agatagctgt cccagtcttt ccataccggc tgcaaaccct gagcggttaa cgcggcagca
     1081 accgcttccg gtctgcgatc gtcgtgcggt gagaactgtt ccaactcggg gtgattatca
     1141 gcatagccac ctggctgcgt tttcgagaag gcgctgacgt tattgatcgc cagcggaata
     1201 acgcgatcgc gaaaccacgg tgattcccgc gtggagagtg acagttcaat ctccggtgca
     1261 agcagtcgga aggcgcagat ggtttgcact aactggcgtt catccataat cgacgcaggc
     1321 tcaatgccgc cagtacatgg acgcaggcgt gggaaggaga cagagtagcg gctttgccag
     1381 taatgctgtt gcagccatag caaatgttct gcaaccatat agcagtcaac tcgccagttg
     1441 tcggaaaggc caattagcgc gccgaggcca atcttatcaa tccccgcacg ccccagccga
     1501 tccggcgttt ccagccgcca gaagaagtcc tgttttttcc ctttcagatg atggcgggcg
     1561 tatgtcgcct cgtgatacgt ctcctgataa accatcacgc catcgagacc aagctgtttt
     1621 agctcggcat attccgcctc cgccagcggt tgtacttcca tctgtagtga agagaactgt
     1681 tcacgcaggg cagggagatg acgacgaaag taatccatcc ccactttcgc ctgatgttca
     1741 ccggtgacta aaagcagatg ttcaaagccc atctcccgta tagcggcgct ttccctggca
     1801 atatccgctt catccagcgt tttgcgcttg atgcggttgc tcatagagaa tccgcagtac
     1861 gtgcagtcgt tagcgcaaag attggaaaga taaagcggga cgtagaaact aactgtgttg
     1921 ccaaatcgct gacgggtcag acgctgcgcc cgttgggcca gttgttccag atagccactg
     1981 gcggctggcg ataacagcgc catcatatcg tcgcgggtga gttgcgaggc atttagcgcc
     2041 cactctacgt cagcagccgt tttgccgttg atacgcaggc ggatgtcgtc ccagtccagt
     2101 tgtcgccagc gatcgctgaa ggttttcata ccgatgcctc cagaaatccg gtcagcgggc
     2161 tggtggcatg agcaaaataa ctgcggctgc ccggtccggc ctgacgtgcc agcaggcctg
     2221 cttctactgc cagacgaaat gccttcgcca tgttgacggg atcgtccgcg acggcaatcg
     2281 ccgtattcac taacaccgcg tcggccccca tttccagcgc ctgcgcggca tggctgggaa
     2341 cgccgatgcc agcatcgaca accaccggaa ctgtggcctg ctggataata atctccagca
     2401 tggcgcgggt ttccagcccc tgattcgagc caatcggcgc gccgagcggc atcaccgctg
     2461 cacagccgac ttcttccaga cgtttacaca ataccggatc ggccccgcag taaggcagca
     2521 cgacaaatcc ctgttgtacc agcgtttcgg cggctttcag ggtttcgatg ggatcgggca
     2581 acagccagcg ggcgtcaggg tgaatctcta attttaacca gtttgtgcct aacgcttcac
     2641 gagccagatg ggcggcgaaa atggcttctt ccgctgtttt cgccccggat gtatttggca
     2701 gcagggtcac acccgccgcg ataagcggtt cgaggatagc gtcgttgtgc tggcgcaagt
     2761 cgacacgttt catcgccagc gtcaccagct ggctgccgga agcgcggatc gcctccacca
     2821 tcagttgtga agaagcgaat ttccctgtgc cggtaaacag atgtgaatca aacgttttgt
     2881 ccgcaatacg taacatctca accccctgca ataacctgaa aaagcaggat ctggtcgcca
     2941 tcctgcacga tatgttgcgt ccactgctca cgcgggacga tttgctgatt aatcgccaga
     3001 gccgcgcccg cttgtcgttg gtcgagttgc tccagtagtt cgtgaacagt ttgcccggcg
     3061 gcgcactgca tcggttgatc gttaaacagg atctgcattg ctccctccgc ataccgggca
     3121 accactggcg cggcgcaacg ccaagctgcg ccactggctc gacttaccgt cgaacagtcg
     3181 gagttctccc gcaggcgtct ctataccgct taataactta atggcttcca gtgcctgcaa
     3241 agtgcccata actccgacca ccgggccaac cacgcccgcc gtgcggcagt tgcgttctgg
     3301 ctcctggtta tctggccaca ggcagcggta acacccctgc tcccagggcg gcgtcagtac
     3361 catcaactga ccgccaaatc cgaccgcgct ggcggtgata agcggcgtgt tgagtgccac
     3421 gcaggcggca ttaatctcct ggcgagtcgc catattgtcg gtacagtcga gcaccacatc
     3481 ggcttgtgca accgcatctt ttaacgcctc acccgttaac cgttgatgta atgctgtcag
     3541 ttgaatatcg ggattcaact gtgtcagtcg ctgatggctg acctgcgatt tcgggcgatc
     3601 gatatcttca gtggtaaaga ggatttgtcg ttgcagattg cttaaatgca catcgtcgtc
     3661 atctgccagt accagcgtcc cgacgccagc gcccgccagg tatagcgcag caggtgtacc
     3721 cagcccgcca agaccgataa tcagcacctg gctgtcgagc agtttttgct gcccgtcaag
     3781 agcgatatcg tcgagcagga tttggcggct ataacgcata aagtcacggt cattcatcgc
     3841 caactcctgc aatttccagc aactgtgccg ttgccaaacg ccagtctgcg gcttgagtaa
     3901 tggcgctgac gacggcgata ctgccgacac ccgttgctat caccgcaggc gcgcgtgcca
     3961 gactgatacc gccaatcgcc acggtgggat aatccgccag tcgctcaaca tgccgtgcca
     4021 gctgttccag cccctgcggt gcagaaggca tctgtttggt ttgcgtcggg aacacatgtc
     4081 ccagcgcgat ataagagggg cgtgctgcca gcgcgacgtc gatttccata tcgtcatggg
     4141 tcgaaacgcc cagtcgcaga cctgccgcgc ggatggcgct gagatcggtg gtttgcaaat
     4201 cttcctgccc caaatggacg ccatacgcct gatgcttgat cgccagccgc cagtaatcgt
     4261 tgataaacaa tcgcgcgtta tagcggcggc ccagcgcaat tgccgccatg acatcggctt
     4321 ccacctcttc atcgcgccga tctttgatgc gtagctggag agtacgtacg cctgcatcca
     4381 acagacgttc gatccactgt acgctgtcca ccaccgggta tagtcctaaa cgaaaaggta
     4441 caggaggaaa atcaggctga tacatcacgc ttcctcctta cgcaggtaga tttctccgcc
     4501 tctggcacgg aagttctccg acatatctgc cattcccatt tcaatagttt gcgtggcggc
     4561 gtaatcacgc acttcctggc tgattttcat cgagcagaat ttcggcccac acatggagca
     4621 aaaatgggcg actttacctg actcttgcgg cagggtttca tcgtgataag cgcgggcggt
     4681 aaacgggtcg agggccagat taaactggtc ttcccagcga aattcgaagc gggctttcga
     4741 catggcgtta tcgcgaattt gcgcgcccgg atgccctttc gccaggtcag cggcgtgggc
     4801 ggcaatctta taggtgataa gcccctgctt aacatcttct ttattgggca gacccagatg
     4861 ctcttttggc gttacgtaac agagcatcgc gcagccaaac cagccaatca tcgccgcacc
     4921 aatccccgac gtgaagtggt catagcccgg cgcaatatcg gtagttagcg gccccagagt
     4981 gtaaaacggc gcttcgtggc agtgctctaa ctcctcggtc atattgcggc ggatcatctg
     5041 catcggcacg tggcctgggc cttcaatcat cacctgcacg tcatattccc aggcaatttt
     5101 ggtcagttcg cccagcgtat gcagctcggc aaactgcgct tcatcgttgg cgtcctgaat
     5161 agaaccgggg cgcagaccgt cgcccagcga cagcgaaacg tcataagcgg cacagatttc
     5221 acaaatttcg cggaagtgtt gatagaggaa attttcctga tgatgggaga ggcaccattt
     5281 cgccataatc gaaccgccgc gagagacgat accggtcagg cgtttcgcgg tcatcggcac
     5341 atagcgcagc agtacgcccg catggatagt gaagtaatcc acaccttgct cggcctgttc
     5401 cagcagcgtg tcgcggaacg cttcccaggt aagatcttcg gcgatcccgt taaccttctc
     5461 cagcgcctgg tagatcggca ctgtaccgat cggcaccggg ctgttacgca aaatccactc
     5521 gcgggtttcg tgaatatagc gaccggtgga gagatccatc accgtatccg ctccccagcg
     5581 cgtggaccat accagctttt ccacttcttc ttcgatggaa gaggtgaccg ccgagttgcc
     5641 gatattggcg ttaactttta ccaggaaatt gcgaccaata atcatcggct ccgattccgg
     5701 atgattaatg ttggccggga taatcgcacg tccggcagca acttcatcac ggacaaattc
     5761 cgcagtgata ttttccggca gacgtgcgcc aaagctcatt cccggatgct ggtggcgtaa
     5821 aacttcgcta cggatgcgct cgcggcccat attctcgcgg atggcgatga attccatttc
     5881 cggcgtgatg atgccctggc gggcgtagtg cagttgggtg acacggcgtc ctgctttggc
     5941 gcgttttggt gttagtacac cgctaaaacg cagttcgtcg aggccatcat ctgccagccg
     6001 cgctttagtg taatcggaac tgcgcacggt aagttcttcg gtatcgccgc gcgcatcgat
     6061 ccacggctgg cgtagttttg ccagcccttg ctgcacgtta atggcaatct gcggatcacc
     6121 atacgggccg gaggtgtcgt agaccggaat cgcttcgttt tcttcgtact gcggctgttc
     6181 tttgctaccg ccaattagcg tcgggctaag ctggatctca cgcatcggca cgcgcacgcc
     6241 ggggtgtgtg ccagtgatat aaatgcgttt tgagttggga aaggcggtgc cttccagggt
     6301 gtcgataaaa tgttgggccc gggcgcgttg ttcgcggcgg gtcagttttg ttgcagacat
     6361 agctcattcc aaaaagttaa ggacgtggct tgtcagacga cggatgaagc aagagacgat
     6421 cgccgcaaag gcgatgcgat agcagattaa ctcttgttcc cttcgcaggt attagcctga
     6481 tcaggttccg cggatcccga ataaacggtc tcagccagtt aaggcactcc gacaagaaat
     6541 tagcccccac aaacggggcg ttgaatgtaa attacgcgtt aacagcgcag aactcaagca
     6601 ggatgtttga cgcgggcggc gtcaagcacc agcagaatca gcttatcttc cagcacaaag
     6661 cgcgcttcca gcgcctcgcc gatgtcagat aaaacctgtt gaaactcaag gtaattatca
     6721 tgatcgatgg cggtttccag gctggaatcg tagtaatcca taatctgttg ggtattggct
     6781 tcgagttgcg gccaaatctt cgcggcgcgc gcgagttgcc cgttgccttc cagcttatga
     6841 agaatgcgct cataaatact gaaatgtccg gcagacaagt aatcgaccag gctctgacaa
     6901 aaatcatcaa gggctttttc gtttagcctc atgtacgatt ctttgccagg cttaatgcca
     6961 accagattgt agtaagccac gagcagatgc ttacgtacat gtagccagcg atcaaccagt
     7021 ttgttacttc ctctgacgcg ctccgtcagg ttatcgagct ggttaagcat gattgactcc
     7081 gcaagtttgt attcaaaaac tgctcagtga gaaatgtaaa aaccatgtta aacatgccag
     7141 tgatgcaaag gtagtgcaag agctatggat cgtataattg aaaaattaga tcacggctgg
     7201 tgggtcgtca gccatgaaca aaaattatgg ttgccgaagg gagaattgcc atatggcgaa
     7261 gcggcaaatt tcgatcttgt gggtcagcgc gcactacaaa tcggcgaatg gcagggggaa
     7321 cctgtttggt tagtacaaca gcagcggcgt cacgatatgg ggtcggtacg tcaggtcatt
     7381 gatctcgatg ttgggctgtt tcaactggcc ggacgaggcg tacaactggc ggagttttac
     7441 cgatcgcata aatactgtgg ttactgcggg catgaaatgt atccgagcaa aaccgaatgg
     7501 gcgatgctgt gcagccattg ccgtgagcgt tactacccgc aaatcgcccc ctgcattatt
     7561 gttgccatcc gtcgcgatga ttcgatcctc ctcgcccagc atacccgcca tcgtaacggt
     7621 gtccatacgg tacttgccgg attcgtcgaa gtgggcgaaa ccctcgagca ggcggtcgcg
     7681 cgggaagtga tggaagagag cggaattaaa gttaaaaact tgcgttatgt gacttcccag
     7741 ccgtggccgt ttcctcagtc tttaatgacc gcgtttatgg cggaatatga cagcggcgac
     7801 atcgtgatcg acccgaaaga attgcttgag gcgaactggt atcgctatga cgatttgccg
     7861 ttactcccgc cgcccggcac cgtagcgcgc cgtctgatag aagatactgt ggcgatgtgt
     7921 cgggcagagt atgagtgatg atacactgac cgcctgacgc actaaggaac agccaaaatg
     7981 accgaactta aaaacgatcg ttatctgcgg gcgctgctgc gccagcccgt tgatgtcact
     8041 ccagtatgga tgatgcgcca ggcgggtcgc tatctaccgg aatataaagc cacgcgcgcc
     8101 caggcgggcg attttatgtc gctgtgcaaa aacgccgagc tggcgtgcga agtgactttg
     8161 caaccgctgc gtcgctaccc gctggatgcg gcgatcctct tttccgatat cctcaccgtg
     8221 ccggacgcga tggggttagg gctctatttt gaagccggag aaggtccgcg ttttacctcg
     8281 ccagtcacct gcaaagccga cgtcgataaa ctgccaattc cggacccgga agatgagctg
     8341 ggttacgtga tgaacgcggt gcgtaccatt cgtcgcgaac tgaaaggcga agtgccgctg
     8401 attggttttt ccggcagccc gtggacgctg gcgacctaca tggtggaagg cggcagcagc
     8461 aaagcgttca ccgtgatcaa aaaaatgatg tatgccgatc cgcaggcgct gcacgctcta
     8521 ctcgataaac tggcgaaaag cgtcactttg tatctgaatg cgcagattaa agccggtgct
     8581 caggcagtga tgattttcga cacctggggc ggtgtgctta ccgggcgcga ttatcaacag
     8641 ttctcgctct attacatgca taaaattgtt gatggtttac tgcgtgaaaa cgacggtcgc
     8701 cgcgtaccgg tcacgctgtt taccaaaggc ggcggacagt ggctggaagc gatggcagaa
     8761 accggttgcg atgcgttggg cctcgactgg acaacggata tcgccgatgc gcgccgccgt
     8821 gtgggcaata aagtcgcgtt gcagggtaat atggatccgt cgatgctgta cgcgccgcct
     8881 gcccgcattg aagaagaagt agcgactata cttgcaggtt tcggtcacgg cgaaggtcat
     8941 gtctttaacc ttggtcacgg cattcatcag gatgtgccgc cagaacatgc tggcgtgttc
     9001 gtggaggcag tgcatcgact gtctgaacaa tatcaccgct aaggagtgat tatggatctc
     9061 gcgtcattac gcgctcaaca aatcgaactg gcttcttctg tgatccgcga ggatcgactc
     9121 gataaagatc caccggatct gatcgccgga gccgatgtcg ggtttgagca gggcggagaa
     9181 gtgacgcgag cggcgatagt gctgctgaaa tatccctcgc ttgagctggt ggagtataaa
     9241 gttgcccgca tcgccaccac catgccttac attccaggtt ttctttcctt ccgcgaatat
     9301 cctgcgctgc tggcagcgtg ggagatgctg tcgcaaaagc cggatttagt gtttgtcgat
     9361 ggtcatggga tctcgcatcc tcgccgtctt ggcgtcgcca gccattttgg cttattggtg
     9421 gatgtgccga ccattggcgt ggcgaaaaaa cggctctgcg gtaaattcga accgctctcc
     9481 agcgaaccgg gcgcgctggc cccactgatg gataaaggcg agcagctggc ctgggtctgg
     9541 cgcagcaaag cgcgctgtaa cccgttgttt atcgctaccg gccatcgggt cagcgtggac
     9601 agcgcgctgg cgtgggtaca acgctgcatg aaaggctatc gtctgccgga gccaacgcgc
     9661 tgggcggacg cggtggcctc ggaacgtccg gcgttcgtgc gctatacagc aaatcagccc
     9721 taattcaggt aaactgcgga taatttccgt atttgagaac tcaacatgtt acaaaaccca
     9781 attcatctgc gtctggagcg cctggaaagc tggcagtatg tcactttcat ggcttgctta
     9841 tgcgaacgca tgtaccccaa ttacgccatg ttctgccagc aaaccggttt tggtgatggg
     9901 caaatttacc gtcgcattct cgatctcatc tgggaaacgc tgaccgttaa agacgcaaaa
     9961 gtaaatttcg acagccaact ggagaaattt gaagaagcga ttccttcagc cgacgatttc
    10021 gatctgtacg gcgtttatcc ggcaatcgat gcatgcgtgg cgttaagtga actggtccat
    10081 tcgcgtttga gtggtgaaac gctcgaacac gcggtggaag tgagtaagac ctccatcacg
    10141 accgttgcga tgctggaaat gactcaggct ggtcgcgaaa tgagcgatga agagcttaaa
    10201 gaaaacccag ctgtagagca agaatgggac attcagtggg aaatattccg acttttagct
    10261 gagtgcgaag aacgcgatat cgagctgata aaaggcctta gggcagacct gcgtgaggcg
    10321 ggtgagagca atattggtat aatttttcag caataagacc agaaaacgtg atttaacgcc
    10381 tgatttgtcg tacctggagt cttccctttc gccccccgtc tggtctacat ttggggggcg
    10441 aaaaaaagtg gctatcggtg cgtgtatgca ggagagtgct attctggcat ttccgtcgca
    10501 ctcgatgctt agcaagcgat aaacacattg taaggataac ttatgaacaa gactcaactg
    10561 attgatgtaa ttgcagagaa agcagaactg tccaaaaccc aggctaaagc tgctctggag
    10621 tccactctgg ctgcaattac tgagtctctg aaagaaggcg atgctgtaca actggttggt
    10681 ttcggtacct tcaaagtgaa ccaccgcgct gagcgtactg gccgcaaccc gcagaccggt
    10741 aaagaaatca aaattgccgc agctaacgta ccggcatttg tttctggcaa ggcactgaaa
    10801 gacgcagtta agtaagattg cgtggcagtg aacagtttta acgaaggggt ggtttcaccc
    10861 cttttgtctt tctggcgtcg atcattgatg ctggcaggtg cgctgtttct cactgcctgt
    10921 agccacaact cttcacttcc tccctttacc gccagtggat ttgctgaaga ccagggcgcg
    10981 gtacgcatct ggcgaaaaga cagcggcgat aatgtgcatc tgcttgccgt gtttagcccg
    11041 tggcgcagtg gcgataccac gacgcgagag tatcgctggc agggcgataa cctcacgctc
    11101 atcaatatca atgtttacag caaaccgccg gtgaatattc gcgcgcgttt tgacgatcgc
    11161 ggtgatctga gctttatgca acgtgaatcc gatggggaaa agcagcagct ttctaacgac
    11221 caaatcgatt tataccgtta tcgtgctgat cagatccgcc agattagcga tgccttacgt
    11281 caggggagag tcgtgctgcg ccaggggcgc tggcatgcga tggaacagac cgtgaccacc
    11341 tgcgaagggc aaaccattaa acctgattta gattcgcagg cgatagcgca tatcgagcgc
    11401 cgccagagcc gctcttctgt tgatgtcagc gtggcatggc tggaagcgcc cgaaggttcg
    11461 caattactgt tggtggcaaa ctctgatttc tgtcgctggc aacccaacga gaaaacgttc
    11521 tgatttacca gtggcccata cccatatgac cgccaccacc gcagccgccg taacccattc
    11581 cggtaccgcg cggaataccc gcttcagcca tcgcaatatc tcgtttcacc cgtaactcat
    11641 ctaacgactg acgcaaattc tccatctctt tggcgaccgc gttaattttg ctgctatccg
    11701 gtgggttcgc ggctaacagg gcattgtatt cataacgctt cgtcaccagt tgctgttgca
    11761 gtgcgctgct ttgagcgtaa aagtcattat ggattttctg ccacgctgtc tgttgttcgc
    11821 tggtcaaagg cgcggcattt tgctgccaca taccgtgtcc gccgtgagca aatgcagatg
    11881 tcgagcccat cgccattgct gaaagcgcca tcattaccag ggcaattttc gtgttccgtt
    11941 tcatggttaa tcctccagtg gttgtcttac ttcgggtatt gcatcttccg tgccaacgat
    12001 gaaacgctga tatgacgggt aatctggcat gataaacgag taaaaatgac tcgcctgctg
    12061 cgggtagcga gtcattttta ctcattgaaa cttaagcctt tgtgttacag cgcagggtaa
    12121 gcgctgataa aagatggcat gatttctgct gtcagaaagg gatgagcagg caaagaagaa
    12181 gatgcgtttt atgcaacgtt ctaaagactc cttagctaaa tggttaagcg cgatcctccc
    12241 cgtggtcatt gttgggctgg tggggctgtt tgcggtgacg gtgattcgcg attatgggcg
    12301 cgagactgcc gccgccagac aaacgctgct ggaaaaaggc agtgtactta ttcgcgctct
    12361 tgaatccggc tcgcgcgtcg gcatggggat gcgcatgcat catgcgcagc agcaggcctt
    12421 actggaagaa atggccgggc agcctggagt acgttggttt gcggtcacgg atgaacaagg
    12481 aacaatcgtg atgcatagca actccggcat ggtgggaaaa cagctttatt ccccgcagga
    12541 aatgcagcag ttacatccgg gagatgaaga agcgtggcgg cggatcgata gcgcagacgg
    12601 cgagcctgtt ctggaaattt atcgccagtt tcaaccgatg tttgctgctg gaatgcaccg
    12661 gatgcgccat atgcagcagt atgccgcgac accacaagca attttcatcg ctttcgacgc
    12721 cagtaatatt gtgagtgccg aagatcgtga gcagagaaac accctgatta tcctcttcgc
    12781 cctggcgacg gtcttgctgg caagcgtgtt gtcattcttc tggtatcgcc gctatctgcg
    12841 ctcgcgccag ctgttgcagg atgaaatgaa gcgcaaagag aagctggtgg cactggggca
    12901 tcttgcagca ggcgttgccc acgaaatccg taatccactt tcctcaatta aagggctggc
    12961 gaaatacttt gccgaacgcg cgccagcagg gggagaagcg catcaattgg cgcaggtgat
    13021 ggcgaaagaa gccgaccgtt taaaccgtgt ggtaagcgag ttgctggaac tggttaagcc
    13081 aacgcatctg gctttgcagg cggtggatct caacacgctg attaaccact cattacagct
    13141 ggtaagccag gatgcaaaca gccgggagat ccagttacgc tttaccgcca acgacacatt
    13201 accggtaatt caggccgacc cagacaggct gactcaggtc ctgttgaatc tctatctcaa
    13261 tgctattcag gcgattggtc agcatggtgt gattagcgtg acggccagcg aaagcggcgc
    13321 gggcgtgaaa atcagcgtta ccgacagcgg taagggaatt gcggcaggtc agcttgaagc
    13381 catcttcact ccgtacttca ccaccaaagc cgaaggcacc ggattggggc tggcggtcgt
    13441 gcataatatt gttgaacaac acggtggtac aattcaggtc gcaagccagg agggaaaagg
    13501 cgcaacgttc accctctgtc ttccggtcaa tattacgcgt aaggacccac aaggatgacg
    13561 cacgataata tcgatattct ggtggtggat gatgacatta gccactgcac tattttgcag
    13621 gctttactgc gcggctgggg ctataacgtc gcgctggcga acagcgggcg acaggcgctg
    13681 gagcaggtgc gggagcgagt ttttgatctt gtgctttgcg atgtgcgaat ggcggagatg
    13741 gacggcattg ccacgctaaa agagatcaaa gcgttaaatc cggcgattcc ggtactgatt
    13801 atgaccgcat actccagcgt cgagacggcg gtagaggcac tgaaaaccgg ggcgctggat
    13861 tatctcatca agccgctgga tttcgataac ctacaggcga cgctggaaaa ggcgctcgcg
    13921 catacgcaca gtattgatgc tgaaacacct gcggtgactg ccagccagtt cggtatggtc
    13981 ggtaaaagcc cggcgatgca acacctgctc agtgaaatcg ccctcgtcgc gccatcggaa
    14041 gccacggtgc tgatccacgg cgattccggc accggtaaag agctggtcgc cagggcgatt
    14101 cacgccagta gcgcacgtag cgaaaaaccg ctggtaacgc tcaactgtgc ggcactcaac
    14161 gaatccttgc tggaatctga attgttcggt cacgaaaaag gggcgtttac tggagccgat
    14221 aaacgccggg aagggcgctt tgttgaggcg gacggcggca cgctgtttct cgatgaaatt
    14281 ggcgatatct cgccgatgat gcaggtgcgt ctgctgcgtg cgattcagga gcgcgaagtt
    14341 cagcgtgtcg gtagcaacca gactatctcg gttgatgtcc ggctgattgc ggcgacccat
    14401 cgcgatcttg ccgcagaggt gaatgccggg cgttttcgcc aggatctcta ctatcgcctg
    14461 aatgtggtgg cgattgaagt accatcgctg cggcaacggc gggaagatat ccctctgctg
    14521 gctggccatt ttctgcagcg ctttgccgag cgtaatcgca aggcggtaaa aggttttacg
    14581 ccccaggcga tggatctgtt gattcattac gactggccgg gaaatattcg tgagctggaa
    14641 aacgcggtgg aacgggcagt ggtgctgctg accggggaat atatttccga acgcgagctg
    14701 ccgctggcga ttgccagtac gccgatcccg ctgggacaaa gtcaggatat tcagccgttg
    14761 gtggaagtgg aaaaagaggt gattctggcg gcgctggaga aaacgggcgg caacaaaacc
    14821 gaagccgccc gtcagttagg gatcacgcgc aaaacgctat tggcaaaact gtcgcgttag
    14881 ttctgctcgc gttcgatggc gcgccagccg atatctttcc ggcagaagca gtcgtcccag
    14941 tgaatatcgg tcattaaggc ataagcgcgt ttctgcgctt ctgccacggt atgacccagc
    15001 gcggtgacgc acagtacgcg cccgccgctg gtcactacct gctcgtcatc cgccagtttt
    15061 gtgcctgcat ggaacacttt gccgtcttcc acttcttcca gcggcaggcc gtggatcaca
    15121 tcaccggtgc ggtaatcgcc cggatatcca cccgcagcca tcaccacgcc gagagaagcg
    15181 cgttcatccc actcggatgt tttctcgtcc agcttccctt cacaggctgc caggcagagc
    15241 tcaaccagat cggacttcat gcgcagcata atcggctggg tttccggatc gccaaagcgg
    15301 cagttaaatt cgataacctt cggattgccc tgtttgtcga tcatcaggcc cgcgtagaga
    15361 aaaccggtgt aggtgttgcc ttccgccgcc atgcctttca cggttggcca gatgatacgt
    15421 tccatggtgc gctgatgaac ttcatcggtt actaccggcg cgggggagta agcgcccatc
    15481 ccgccggtgt ttggtccggt atctttatcg cctacgcgtt tgtgatcctg gctggtagcc
    15541 atcggcagca catgctctcc gtccaccatc acgataaagc tcgcttcttc gccatcgagg
    15601 aactcttcga taacgatgcg atgacccgcg tcgccaaaag cgttgcccgc cagcatatcg
    15661 tgaacagccg cttccgcttc ttccagcgtc atcgccacga taacgccttt cccggcagcc
    15721 agaccgtccg ctttaatgac gattggcgcg cctttctcac gcagatacgc cagcgcaggt
    15781 tctacctcgg tgaagttctg gtattccgcc gtagggatct tatggcgggc caggaaatct
    15841 ttggtaaacg cttttgagcc ttccagttgg gccgcacctg cggttgggcc gaagattttc
    15901 agcccggcgg cgcggaaggt atcgaccacg cctttcacca gcggcgcttc cgggccgacg
    15961 atggtcagat caatcttttc gttttgtgcg aaatccaaca gcgccgggat atcggttacg
    16021 ccgatagcga cgttctgtag cgcgggttcc agcgcagtgc ctgcattacc cggagcaaca
    16081 aaaacagttt caaccagcgg cgactgcgcc gctttccagg ccagcgcgtg ctcgcgcccg
    16141 ccgttaccaa tcactaatac tttcatctat tgctccatta atggcggaag tggcgcatgt
    16201 cggtgaagag catcgcaata ccgtgctcgt cggcggcggc aatcacttcg tcatcacgga
    16261 tagaaccgcc aggctggatt acgcaggtca cgcccgcagc ggcggcggca tcaataccgt
    16321 cgcggaacgg gaagaacgcg tcagaagcca tcgaggaacc tttcacttcc aggccttcat
    16381 cggccgcttt aataccggcg atttttgcgg agtacacgcg gctcatctgg cccgcgccaa
    16441 tgccgatagt catattgttt ttggcataga cgatagcgtt ggatttcaca aacttcgcca
    16501 ccttccagca gaacagcgca tcacgcagtt cctgttcgct cggctgacgt ttggtcacca
    16561 cgcgcagttc ttccgcaccg accatcccca ggtcacgatc ctgaaccagc agaccgccgt
    16621 tcacgcgttt gaaatcgagg cccggaacac gctcgcccca ctgaccgcag gtcagaacgc
    16681 gtacgttctg tttggcggcg gtgattttca gggcttcttc gctggcggac ggcgcaataa
    16741 tcacttcgac aaactgacga gaaatgatgg cctgtgcggt ttccgcatcc agctcgcggt
    16801 taaaggcaat gatgccgccg aatgcggagg ttgggtcggt tttgtacgcg cgatcgtaag
    16861 catcaagaat ggaattgccg atagccacgc cgcaagggtt ggcgtgcttc acaatcacac
    16921 atgccggctc ggcgaactct ttcacgcact ccagcgccgc atcggtatcg gcgatgttgt
    16981 tataagagag ggctttaccc tgaacctggg ttgcggtagc aacggaggct tctttcacat
    17041 tctcttctat atagaaggca gcctgctggt ggctgttctc gccgtaacgc atatccagct
    17101 tcttaatgaa gttcaggttc agcgtgcgtg ggaagcgacc ggcggcttct ttgctttcac
    17161 cgtggtaagc cggaaccatg ctgccgaagt agttggcaat catgctgtcg taggcggcag
    17221 tgtgttcgaa ggctttgatg gcgaggtcga aacgggttgc aagcgtcagc gatccttcgt
    17281 tgtcatccat ctctttaata atggcgtcat agtcgctgct cttcaccacg attgcgacat
    17341 ctttatggtt cttggcggcg gagcgcacca tcgttgggcc gccgatatcg atgttctcaa
    17401 ccgcatcttc cagcgagcaa ccttcacggg ccacggtctg ggcgaacgga tacaggttaa
    17461 caaccaccat atcgataggc tggatctgat gttcttccat aatggcatcg tcctggccgc
    17521 gacggcccag aatgccacca tgtactttcg gatgcagggt cttcacgcgt ccatccatca
    17581 tctccgggaa accggtgtaa tcggaaactt cggttaccgg cagacctttt tctgctaaca
    17641 gacgggcagt gccccctgta gacagcagct ccacaccgcg tgcggaaagt gcctgggcga
    17701 attcgacgat accggctttg tcagaaacac tgagcagagc gcggcggact ggacgacgtt
    17761 gttgcatggt aaatcccctg gatttgacta ttacagagag cgttagctga atttttcgcg
    17821 aaaaactcag ctaacgcccc taacggggca tccttatttt tcgcccgcat tgtaacgaaa
    17881 acgtttgcgc aacgctcgcg aatttttctc tttcaatggt gatcacaatt ttgacagtgg
    17941 ttaccgtggg aaaaatacag aaattacatt gatgattgtg gataactctg tgcgtaaaaa
    18001 ggtataaagc ggggttttgc tggggaatgc agcagtcagt tatttttctg caatttttct
    18061 attgcggcct gcggagaact ccctataatg c
//
LOCUS       DAJOSY010000086        17969 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJOSY010000086 DAJOSY010000000
VERSION     DAJOSY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.086"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..272)
                     /locus_tag="OHN27_003167"
     CDS             complement(<1..272)
                     /locus_tag="OHN27_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HCQ2287556.1"
                     /translation="MEQLLADYKKGNVILFVGAGVSMNLGLPSWSQLVDHIATELGYD
                     PDIYRTFGSALELAEYYKLKKGKIGPLRSWMDRMWHSSDIDINKSKV"
     gene            complement(272..826)
                     /locus_tag="OHN27_003168"
     CDS             complement(272..826)
                     /locus_tag="OHN27_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HCQ2287557.1"
                     /translation="MNIRFITRNRNKIKEINKILSGTGVVVLASEHSIDEIQTENVHA
                     LIKDKLLKAFKLVGRPVFVEHTGLYIESLNGFPGGLTQIFWDKLQADKFSQLLGTSEN
                     PRLVAKTIIGYCDSMKIYIFEGETQGTISPVPKGPRDFQWDCIFIPDGESETFAEMGD
                     RKNEISMRKKAFDKFKEYLLEGGK"
     gene            complement(823..1803)
                     /locus_tag="OHN27_003169"
     CDS             complement(823..1803)
                     /locus_tag="OHN27_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCQ2287558.1"
                     /translation="MDLYEARMMDKGELHVHLNGLVSTQVIRGVLEKNIIEMPEHFNI
                     DTDLNVTHPARSLVDYLKPWQFLRLIPASRFDLSIMIESAFSNLIAENIKFVELRNSI
                     IYIALLNNISVSEALKWVLDDIDFFSKKFNIQAGLILTITRGEYAPEHLRSLLGAYKS
                     LGCPAGVIGLDLAGNEDITPPPETASLFRSAKDKYGLGITIHAGETGNADNIRNAIIS
                     YGADRIGHGTAVIRSPEIMDLIRELDICIEVCPISNRLTGAVSDVDPHPVGEMINHNI
                     PFVICSDNPAIHRSSLSEDYVAFMQETKNFIVMDNMYETQKRYSFLKGVK"
     gene            2018..2551
                     /locus_tag="OHN27_003170"
     CDS             2018..2551
                     /locus_tag="OHN27_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCQ2287559.1"
                     /translation="MVFIRIHGICCCPHDVGCDGAGTTSPGETMKRERVVLREGAVGL
                     SIDDVAKEAGVSKATVMYDHKSKQDLLSDLISRLMPVEEERVQQAIAESNNTPHPALY
                     GRIISASNALSHTERAVVLAVSESLTNDTNIRDTMRSCTNKDLDEIKRGPKPDVAVLV
                     YLALSGIYCQEFFDFYK"
     gene            complement(2825..3202)
                     /locus_tag="OHN27_003171"
     CDS             complement(2825..3202)
                     /locus_tag="OHN27_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCQ2287560.1"
                     /translation="MAKVFTPEEREKIKGQVVELVPLSGRETLRALEAKTGASRYYIS
                     TLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRTKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECLKSEVMQ"
     gene            complement(3218..3979)
                     /locus_tag="OHN27_003172"
     CDS             complement(3218..3979)
                     /locus_tag="OHN27_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCQ2287561.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPAQAELPEEITTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQGGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     TAGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQITDSSRHVASEK"
     gene            complement(4002..4343)
                     /locus_tag="OHN27_003173"
                     /pseudo
     CDS             complement(4002..4343)
                     /locus_tag="OHN27_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4514..4669
                     /locus_tag="OHN27_003174"
     CDS             4514..4669
                     /locus_tag="OHN27_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaF family protein"
                     /protein_id="HCQ2287562.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LEKHTVS"
     gene            complement(4666..5154)
                     /locus_tag="OHN27_003175"
     CDS             complement(4666..5154)
                     /locus_tag="OHN27_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein B"
                     /protein_id="HCQ2287563.1"
                     /translation="MLDVFTPLLKLFANEPLERLMYTIIIFGLTLWLIPKEFTVAFNA
                     YTEIPWLFQIIVFAFSFVVAISFSRLRAHIQKHYSLLPEQRVLLRLSEKEIAVFKDFL
                     KTGNLIITSPCRNPVMKKLERKGIIQHQSDSANCSYYLVTEKYSHFMKLFWNSRSRRF
                     NR"
     gene            5596..5817
                     /gene="kilR"
                     /locus_tag="OHN27_003176"
     CDS             5596..5817
                     /gene="kilR"
                     /locus_tag="OHN27_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HCQ2287564.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTFITDRMIKVFLGRDGLPVKAESW"
     gene            5817..5987
                     /locus_tag="OHN27_003177"
     CDS             5817..5987
                     /locus_tag="OHN27_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287565.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            6062..6337
                     /locus_tag="OHN27_003178"
     CDS             6062..6337
                     /locus_tag="OHN27_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein RacC"
                     /protein_id="HCQ2287566.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            6439..9039
                     /gene="recE"
                     /locus_tag="OHN27_003179"
     CDS             6439..9039
                     /gene="recE"
                     /locus_tag="OHN27_003179"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCQ2287567.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHAKPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDDDKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIDPGIYYGISNENYHAGPGISKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNAGKEEE
                     KAFLMECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETG
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLSDCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            9032..9841
                     /gene="recT"
                     /locus_tag="OHN27_003180"
     CDS             9032..9841
                     /gene="recT"
                     /locus_tag="OHN27_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HCQ2287568.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNSDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSLSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            9898..10092
                     /gene="ralR"
                     /locus_tag="OHN27_003181"
     CDS             9898..10092
                     /gene="ralR"
                     /locus_tag="OHN27_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HCQ2287569.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            10085..10294
                     /gene="rcbA"
                     /locus_tag="OHN27_003182"
     CDS             10085..10294
                     /gene="rcbA"
                     /locus_tag="OHN27_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HCQ2287570.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSSRIALYSN"
     gene            10373..10588
                     /gene="xisR"
                     /locus_tag="OHN27_003183"
     CDS             10373..10588
                     /gene="xisR"
                     /locus_tag="OHN27_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HCQ2287571.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            10590..11825
                     /locus_tag="OHN27_003184"
     CDS             10590..11825
                     /locus_tag="OHN27_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCQ2287572.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            11877..12812
                     /gene="ttcA"
                     /locus_tag="OHN27_003185"
     CDS             11877..12812
                     /gene="ttcA"
                     /locus_tag="OHN27_003185"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCQ2287573.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12941..14314)
                     /gene="dbpA"
                     /locus_tag="OHN27_003186"
     CDS             complement(12941..14314)
                     /gene="dbpA"
                     /locus_tag="OHN27_003186"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCQ2287574.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIATLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(14792..15775)
                     /gene="zntB"
                     /locus_tag="OHN27_003187"
     CDS             complement(14792..15775)
                     /gene="zntB"
                     /locus_tag="OHN27_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCQ2287575.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            16030..17262
                     /gene="dgcM"
                     /locus_tag="OHN27_003188"
     CDS             16030..17262
                     /gene="dgcM"
                     /locus_tag="OHN27_003188"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCQ2287576.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAAKLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKSLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKDDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(17283..17846)
                     /gene="smrA"
                     /locus_tag="OHN27_003189"
     CDS             complement(17283..17846)
                     /gene="smrA"
                     /locus_tag="OHN27_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCQ2287577.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         5185 a         4203 c         4039 g         4542 t
ORIGIN      
        1 actttagact tattgatatc tatatcgcta gagtgccaca ttctatccat ccagcttcta
       61 agaggcccta tcttgccttt tttcaacttg taatactcag ctaactctag tgcactaccg
      121 aatgtacgat atatatctgg gtcataacct aactcagttg caatgtgatc aaccaattga
      181 ctccatgatg gcaaacctaa gttcattgac acaccagcac caacaaaaag gataacattt
      241 ccttttttat aatcagcaag taattgctcc attattttcc tccctctaat agatattctt
      301 taaatttatc gaaagctttt ttcctcatag atatttcatt tttcctgtcc cccatctcag
      361 caaaagtctc actttcgcca tcaggaataa atatacaatc ccattgaaaa tctcgagggc
      421 cttttggcac aggagatatc gtaccttgag tttcaccttc gaaaatatag attttcatcg
      481 aatcacaata gccgattatt gttttagcaa ctaaacgagg gttttcactt gttcctaata
      541 attgtgaaaa tttatcagct tgaagcttat cccaaaatat ttgcgttaag ccacccggga
      601 aaccattgag gctttctata tatagtccag tatgttcgac aaagaccggt cttccaacta
      661 atttaaatgc ttttaataat ttatctttaa ttaacgcatg gacattttca gtttgaattt
      721 catctataga gtgttcagat gctaatacaa ctactcctgt acctgataga attttattta
      781 tttctttaat tttatttcta tttctggtta tgaatcgaat gttcatttca cacctttaag
      841 aaagctatat cgcttttgag tttcatacat attatccatg actataaaat tcttggtctc
      901 ttgcataaat gcgacataat cttcgctcaa gcttgatctg tgaatagctg gattatctga
      961 gcaaatcaca aacggaatat tatggttaat catttcgcca actggatgag gatcgacatc
     1021 agagactgca cctgtcaaac gattactaat aggacaaacc tcaatgcaaa tgtccagttc
     1081 tctaatcaaa tccattatct ccggagatct tataacagca gtaccatgtc cgatacgatc
     1141 agctccataa ctaattatgg cattccttat gttatcagca tttccagtct cacccgcatg
     1201 aattgttatg cctagtccat atttgtcttt agcacttcta aataaacttg cagtctcagg
     1261 tggtggtgtt atatcttcat taccagccaa gtccaaacca atcacccctg ctggacatcc
     1321 tagacttttg taagcaccaa gaagagaacg aagatgttcg ggagcatatt caccacgcgt
     1381 aatagttaga ataagcccgg cttgaatgtt gaatttttta gaaaaaaagt caatatcatc
     1441 taaaacccac ttcaatgctt cggaaacaga aatgttatta aggagtgcta tgtatataat
     1501 gctatttctt aattcaacaa acttaatatt ctcagcaatt agatttgaaa atgcactctc
     1561 aatcataata gataaatcaa agcgacttgc aggaatgagc cttaaaaact gccatggctt
     1621 taaataatcc actagtgatc ttgctgggtg tgttacattc aagtcagtat ctatattaaa
     1681 gtgttcaggc atttcaataa tatttttttc taggaccccc ctaattactt gagtgcttac
     1741 caatccattc aaatgaacat gtaattctcc tttatccatc attcgagctt cgtaaagatc
     1801 cattttaatt accttatata tccaaactct taattttctc ttttttacca taggattaat
     1861 ccaaaataaa gaccttgttg actaaaccct catgtacaaa atgcacactt actcaaaagg
     1921 atctggaggc aatctcgacc tccatgattt agttcctagg gataagcaga aatctagcac
     1981 tggattaaaa tttaaaaata ataatcaata ggttataatg gtttttattc gcatccatgg
     2041 gatctgctgc tgcccgcatg atgttggatg cgacggcgct ggcacgacct cacccggtga
     2101 aacgatgaaa cgcgaaaggg ttgttcttcg ggaaggtgca gttggacttt caatcgatga
     2161 cgttgcaaaa gaggctggcg tcagtaaggc aacggtaatg tacgatcata agtcaaagca
     2221 agatttgctc agcgatctca tcagtcgcct tatgccagta gaggaggaac gtgttcagca
     2281 agcgatagcg gaaagtaata ataccccaca tcccgcactc tatgggcgaa ttatttcagc
     2341 cagtaatgct ctcagtcata ctgagagagc agtagtgctg gcggtcagcg aatcgttaac
     2401 taacgatacg aatatcaggg acaccatgcg ttcctgcaca aacaaagatt tggatgagat
     2461 aaaaagagga ccaaaaccag acgttgccgt cctggtatat ttggcgttat ctggtatcta
     2521 ctgccaagaa ttttttgatt tttacaaatg atctcctggc gagcgagata aaattatcga
     2581 aggcattcgc tgtttgtatg aatcataccc ttacaagtct gaataaataa tgtctccggg
     2641 aatgacattt catgtgcgac taattcgcga tattcctata cgtaaaggtc cgcttttggc
     2701 acaaagtgga caaccacgct agctctaccc tgtgccacaa aatgtcaatt tgcatctgaa
     2761 ctaatgcact ttaatctcgt cacttcaata aataccgaac atccccctga taaaacgaca
     2821 atattcactg cataacttca ctcttcaggc actcgcgaca gattatgttc agacgcctgt
     2881 cgtaacggcg tatttctccg tctggtaatg accagataag gtcaggatca accacaaccg
     2941 gtttcttcac ctttgtcctt gatagttttt tgcgggcgtt ttgccagtct ttacgcgcct
     3001 gctcagacgg gaataatccg tagcctgaat tgtaaacatc accactggcg actagttctc
     3061 tggcgagagt gcttatgtaa taccttgatg caccggtttt agcctccaga gcccgtaacg
     3121 tctcgcgacc gctcagaggt acaagttcaa caacctgtcc tttaattttt tctcgctctt
     3181 ctggtgtaaa tacttttgcc ataggtgcct ccggcaatca cttttccgat gcaacatggc
     3241 gggaagaatc agtaatctgt cgtacaatat ccctgtgctt gttcaactca cgcagcgcgg
     3301 cgcagacacg ctcccacttc tggacatgat ttttcgcccg acgcagttcg cggtttgcca
     3361 tatgcagtga tggtaaaacc aggtcatccg ctcgcgtttc agtaaacgat ggcagcgact
     3421 gcacaatgtc cgccacagtt tctgttttaa tatcttcctg tgttgcagcc tcctgtactg
     3481 gtaacgcaac acctgcggtc tgaggaaagg ccttaccatc agtttccgct accgatgctg
     3541 ctttcggctc tgctggtaaa ttatcgcccg gtatgcagta acgaaattta ccgccctgat
     3601 ttacgcgaat cagacgacct ttgctgattg ccattgccag cgttgaagcc actttgcgtg
     3661 atgtggtacc aaacaatgta gccagctcat cagccgtttg tggtcctcgt tgttcaatcg
     3721 tcgcggttaa atcgcactct gagattttcg ctactgttgc tgtggtgatt tcttccggca
     3781 gttctgcctg cgctggctgt tcctgctgaa cattgttatc agccacacgc caggtgtacg
     3841 cgcttttatc aacaaaacca gcctttttca gttcccatag ttcgttcagc acttcttcac
     3901 gactgatatc aagtcgcgca gcaagttcta tggatgtggc ttttcccatt gctttcagtg
     3961 cgtcaaaaac agtctccatt aaatttttct cccggtaaaa attacttcgc aattcctggc
     4021 tggacgacat tcggacgcca gctctcccag ttaaaattca cccatcgccc gccgttcatg
     4081 gtcatgcgat ccataatccg ctcgccgagc aatgttttca tggcctcata gttcaggttt
     4141 gtcagcatcc ccacgctgcg catcgacgct gtccggcgat caacaatctg gtgcagtacc
     4201 acctgctcgt ttttcgtctc gcgctgaatg ccaatttcat caagaaccag cagatccact
     4261 tcgcacagtt cccgcaaaaa tttttcgcct gactgcccat cgtcatagct ggcgtgcagg
     4321 gcactcataa catcagccac ggtacccggc gttaagggga gagataagat ggtgcattac
     4381 gaagtagttc agtatttgat ggattgttgc ggtatcactt acaaccaggc tgtgcaggct
     4441 ttacgcagca acgactggga tctctggcag gcagaagtcg ctatacgtag caacaagatg
     4501 tgagattcgc aaaatgcaaa aaatcgacct cggcaacaac gaatccctgg tgtgcggcgt
     4561 gttccccaac caggatggaa cgttcactgc catgacgtat accaaaagca aaacatttaa
     4621 aaccgaaact ggtgcgcgcc gatggttgga gaagcacaca gtaagctaac gattaaaacg
     4681 tctactcctg ctgttccaga ataacttcat aaaatgggag tatttttcgg tgacgagata
     4741 ataagaacag tttgcgctat cactctgatg ttgaatgatg cccttccgtt ctaatttttt
     4801 cataaccggg ttacggcaag gagaagtgat aataagattt cctgttttaa ggaaatcttt
     4861 aaatacagcg atttctttct cagataaacg aagcaatact cgttgctctg gtagtaatga
     4921 ataatgcttt tgaatatgtg ctcgcaatct tgagaaggaa atggcgacca cgaaagaaaa
     4981 ggcaaaaacg ataatctgaa agagccaagg tatttcagta taagcattga atgcgacagt
     5041 aaactctttc ggtatcagcc agagagtgag accaaaaatg ataatcgtat acataagtct
     5101 ttcgagtggc tcgttagcaa aaagtttcaa caatggagta aatacatcca acatatcaat
     5161 aactctcaac tgtaagggta ttgaaatgtt aacacaagct ctcgctgtag gggtatagcc
     5221 gagaccaccg aagcccggag gtggtgaaat aaaaccgggc acaacacgaa ggcgcatttc
     5281 cgatatccat aaagagtcgg tcttgtctgt taaatttaaa tggtgggagt gcgcctccgg
     5341 ttgtaaataa cgacattgct gtgtgtagtc ctggcggcat cagttttttt cttgaagttc
     5401 ggctgatgtc cgcccttttt aaagtgaatt ttgtgatgcg gtgaatgcgg ctaagcgcac
     5461 gtggcacagt taaaagtcat gttagtcctt attggtttgg gtgggaaagc cgactgtaat
     5521 tgttaactgg ttgcagtcac ctggaggcac cagacaccgc atcaacaaag ttcatttgta
     5581 aaaatggaga taattatgat tgcacatcac ttcggaactg atgaaatacc acgtcagtgt
     5641 gtgactcctg gcgattatgt tcttcatgaa ggccggacat atattgcctc ggcaaacaat
     5701 attaaaaagc gaaaactata tattcgtaac ctgaccacaa aaacattcat tactgaccgc
     5761 atgattaaag tcttcctcgg tcgtgatggt ttacctgtaa aggcggagtc atggtgatga
     5821 ctaagaaaat aaaatgtgct taccaccttt gcaaaaaaga cgttgaagaa agcaaagcta
     5881 ttgaaagaat gcttcacttc atgcacggga ttttatcaaa agacgaaccg agaaaatatt
     5941 gcagtgaagc ttgtgccgaa aaagaccaga tggcacatga actttaatta attgactatt
     6001 cgaaactgaa tttatgccag aaatggcagg tattcgctca accttaatta aggagaaaaa
     6061 catgattacc aattatgaag ccactgttgt aactaccgat gacattgttc acgaggtgaa
     6121 tctggaagga aagcgcattg gctacgtaat taaaacagaa aataaagaaa ccccattcac
     6181 tgtggttgat atcgatggtc catcaggcaa cgtaaaaaca cttgatgaag gtgtcaaaaa
     6241 aatgtgcctg gtgcatatcg gaaagaatct gcccgcagaa aaaaaagccg aatttctggc
     6301 aactctaatt gcaatgaaat taaaaggtga aatctgaaag aaatagcctg cgtatggcgc
     6361 aggctatgaa cagtgtgtat ccggcaagat cattcactga acaaaacgaa ttttaatctg
     6421 agttgaggtt aaaaaacaat gagcacaaaa ccactcttcc tgttacggaa agcgaaaaaa
     6481 tcatccggtg aacctgacgt cgtcctgtgg gcaagcaacg attttgaatc gacctgtgcc
     6541 actctggact acctgatcgt taagtcaggt aaaaaactga gcagctattt taaagctgtt
     6601 gccacgaatt ttcctgtcgt taatgacctg cccgctgaag gtgagatcga ttttacctgg
     6661 agtgaacgct atcaactcag caaagactcc atgacatggg aactaaaacc gggagcagca
     6721 ccagacaacg ctcactatca aggcaatacc aacgtcaacg gcgaagacat gactgagatt
     6781 gaggagaata tgctactccc aatttctggc caggaactgc ccattcgttg gcttgctcaa
     6841 cacggcagcg aaaaaccggt aacgcacgtt tcacgcgacg gactccaggc attacacatt
     6901 gctcgggctg aagaactacc ggctgttact gccctggctg tttcccacaa aaccagcctg
     6961 ctcgacccgc tggaaattcg cgaactccac aaactggttc gtgacactga caaagttttc
     7021 cctaatcctg gtaattcaaa cctgggactg ataactgctt ttttcgaagc atacctgaac
     7081 gctgactaca ccgatcgagg actgctgaca aaagagtgga tgaagggtaa tcgtgtttca
     7141 cacatcactc gcacggcttc cggtgctaat gctggcggcg gaaacctcac cgatcgcggc
     7201 gaaggtttcg tacacgatct gacgtcactg gcgcgcgacg tagccactgg cgtactggcc
     7261 cgttcaatgg atctggacat ctataacctt catccggcac acgctaaacg cattgaggaa
     7321 attatcgctg aaaataaacc gcccttttct gttttccgcg acaaattcat caccatgcct
     7381 ggcgggctgg attattcccg cgccatcgtg gttgcgtccg taaaagaagc accaattggg
     7441 atcgaggtca tccccgcgca cgtcactgaa tatctgaaca aagtactgac tgaaaccgat
     7501 catgccaaac ctgatccgga aatcgtggat attgcctgcg gtcgctcctc tgccccgatg
     7561 ccgcagcgag taacagaaga aggaaaacag gatgatgaag aaaaaccgca accatctgga
     7621 acaacggcag ttgaacaggg agaggctgaa acaatggaac cggacgcaac tgaacatcat
     7681 caggacacgc agccgctgga tgctcagtca caggtaaatt ctgttgatgc gaaatatcag
     7741 gaactgcggg cagaactcca tgaagcccgg aaaaacattc catcaaaaaa tcctgtcgat
     7801 gacgataaat tgcttgctgc atcacgtggt gaatttgttg acggaattag cgacccgaac
     7861 gatccgaaat gggtaaaggg gatccagact cgcgattgtg tgtaccagaa ccagccagaa
     7921 acggaaaaaa ccagcccaga tatgaatcaa cctgagccag tagtgcaaca ggaaccggaa
     7981 atagcctgca atgcctgcgg ccagactggc ggggataact gccctgactg tggtgcggtg
     8041 atgggcgacg caacatacca ggaaacattc gatgaagaga gtcaggttga agctaaggaa
     8101 aatgatccgg aggaaatgga aggcgctgaa catccgcaca atgagaatgc tggcagcgat
     8161 ccgcatcgcg attgcagtga tgaaactggc gaagtcgcag atcccgtaat cgtagaagac
     8221 atagatccag gtatttatta cggaatttcg aatgagaatt accacgcggg tcccggtatc
     8281 agtaagtctc agctcgatga cattgctgat actccggcac tatatttgtg gcgtaaaaat
     8341 gcccccgtgg acaccacaaa gacaaaaacg ctcgatttag gaactgcttt ccactgccgg
     8401 gtacttgaac cggaagaatt cagtaaccgc tttatcgtag cacctgaatt taaccgccgt
     8461 acaaacgccg gaaaagaaga agagaaagcg tttctgatgg aatgcgcaag cacaggaaaa
     8521 acggttatca ctgcggaaga aggccggaaa attgaactca tgtatcaaag cgttatggct
     8581 ttgccgctgg ggcaatggct tgttgaaagc gccggacacg ctgaatcatc aatttactgg
     8641 gaagatcctg aaacaggaat tttgtgtcgg tgccgtccgg acaaaattat ccctgaattt
     8701 cactggatca tggacgtgaa aactacggcg gatattcaac gattcaaaac cgcttattac
     8761 gactaccgct atcacgttca ggatgcattc tacagtgacg gttatgaagc acagtttgga
     8821 gtgcagccaa ctttcgtttt tctggttgcc agcacaacta ttgaatgcgg acgttatccg
     8881 gttgaaattt tcatgatggg cgaagaagca aaactggcag gtcaacagga atatcaccgc
     8941 aatctgcgaa ccctgtctga ctgcctgaat accgatgaat ggccagctat taagacatta
     9001 tcactgcccc gctgggctaa ggaatatgca aatgactaag caaccaccaa tcgcaaaagc
     9061 cgatctgcaa aaaactcagg gaaaccgtgc accagcagca gttaaaaata gcgacgtgat
     9121 tagttttatt aaccagccat caatgaaaga gcaactggca gcagctcttc cacgccatat
     9181 gacggctgaa cgtatgatcc gtatcgccac cacagaaatt cgtaaagttc cggcgttagg
     9241 aaactgtgac actatgagtt ttgtcagtgc gatcgtacag tgttcacagc tcggacttga
     9301 gccaggtagc gccctcggtc atgcatattt actgcctttt ggtaataaaa acgaaaagag
     9361 cggtaaaaag aacgttcagc taatcattgg ctatcgcggc atgattgatc tggctcgccg
     9421 ttctggtcaa atcgccagcc tgtcagcccg tgttgtccgt gaaggtgacg agtttagctt
     9481 cgaatttggc cttgatgaaa agttaataca ccgcccggga gaaaacgaag atgccccggt
     9541 tacccacgtc tatgctgtcg caagactgaa agacggaggt actcagtttg aagttatgac
     9601 gcgcaaacag attgagctgg tgcgcagcct gagtaaagct ggtaataacg ggccgtgggt
     9661 aactcactgg gaagaaatgg caaagaaaac ggctattcgt cgcctgttca aatatttgcc
     9721 cgtatcaatt gagatccagc gtgcagtatc aatggatgaa aaggaaccac tgacaatcga
     9781 tcctgcagat tcctctgtat taaccgggga atacagtgta atcgataatt cagaggaata
     9841 attcagcctg gcggtgtaat gcaccgccaa cttgaaatat tttttatgag aaaaattatg
     9901 agatatgaca atgttaaacc atgtccattt tgtggttgtc catcagtaac ggtgaaagcc
     9961 atttcaggat attaccgagc gaagtgtaac ggatgcgaat cccgaaccgg ttatggtgga
    10021 agtgaaaaag aagcactcga aagatggaat aaacgaacca ctggaaataa taatggaggt
    10081 gttcatgtat aaaattaccg ccactattga aaaggaaggt ggcactccta ctaactggac
    10141 aagatattca aaatctaaac taacgaaatc agaatgcgaa aaaatgctct caggtaaaaa
    10201 agaagcaggc gtttccagag agcagaaagt aaaactgata aattttaatt gcgagaaact
    10261 tcagtcctcg agaattgcat tgtattcaaa ttaaaacttc atagctgatt attaataatc
    10321 aacatcgggc gtcaatttca gtctaacatt ggcgcctgcc agaggtgatg cgatggcaca
    10381 agtaatcttt aatgaagagt ggatggttga atacggcctg atgcttcgca ctggtctggg
    10441 ggccagacaa attgaagcat accgccagaa ctgttgggtg gagggcttcc acttcaaacg
    10501 agtatctcct ttaggtaagc cagacagcaa acgagggatt atctggtaca actatccaaa
    10561 gataaatcag tttatcaaag actcatgata tgtctaaatt accaacaggt gtcgagatta
    10621 gaggtagata cattcgcatc tggttcatgt ttcgaggaaa acgatgtcgg gaaacattaa
    10681 aaggctggga gattacaaac agtaatatta aaaaggccgg aaatttaaga gcgctgatag
    10741 ttcatgaaat aaactccggt gaatttgagt atttaagacg ttttccccag tccagcactg
    10801 gggcaaaaat ggtgacaacg agagtcataa aaacgttcgg agagctttgt gatatctgga
    10861 caaaaattaa agagacagag ttaacaacaa acacaatgaa gaaaacgaaa tcacaattaa
    10921 aaacactcag aataataatt tgtgaaagta ccccgatatc acatattcgt tatagcgata
    10981 tcttaaacta ccggaatgaa ctgctgcatg gagaaacgct ttacctggat aatccaagat
    11041 ccaacaaaaa aggaagaacc gtgcgcacag ttgataacta tatcgccctg ctctgttcgc
    11101 tgttgcgttt tgcgtatcag tcgggattta tatcaaccaa accatttgaa ggagtaaaaa
    11161 aattacagcg aaacagaata aagcctgatc cgttatctaa aacagaattc aatgcattaa
    11221 tggaaagtga aaaaggacag agccagaact tgtggaaatt tgccgtttac tcaggacttc
    11281 gtcacgggga actggcagct ctggcgtggg aggatgtgga tctcgaaaag ggaatagtga
    11341 atgtcagaag aaacctgacg atacttgata tgttcggtcc cccaaaaaca aatgccggga
    11401 tccgaacagt aacactactg cagcctgctc ttgaagcact gaaggagcaa tacaaactga
    11461 ccgggcatca tcgcaaaagc gaaatcacct tttatcatcg ggagtacggc agaaccgaaa
    11521 agcaaaaact gcattttgtt ttcatgccca gggtgtgtaa cggaaaacaa aaaccttatt
    11581 actcggtaag cagtttgggg gcaaggtgga atgcagcagt aaaacgtgct ggtattcgcc
    11641 gccgtaatcc gtaccatacg cggcatactt ttgcctgctg gctgttgacg gcaggagcga
    11701 acccggcatt tatagccagc caaatggggc atgaaactgc gcagatggtg tatgaaattt
    11761 acggtatgtg gattgatgac atgaacgacg aacagatagc catgttgaat gcgcggttat
    11821 cgtagttgca aagtttgccc ccaatttgcc ccatttagta ccagagaact gaaataatgc
    11881 aagaaaatca acaaattaca aagaaagaac aatacaacct gaacaaatta caaaaacgtc
    11941 tgcgtcgtaa cgtgggcgaa gccattgctg acttcaatat gattgaagaa ggcgatcgca
    12001 tcatggtttg cctctccggg ggtaaagaca gctataccat gctggagatt ctgcgcaatt
    12061 tgcagcaaag cgcgccaatc aatttttcgc tggtggctgt taacctcgat caaaagcaac
    12121 cgggcttccc ggaacacgtt ctgcccgagt atcttgaaaa gctgggcgtt gagtacaaga
    12181 ttgttgaaga gaatacttac ggtatcgtga aagagaagat tccagagggc aaaaccactt
    12241 gctcactgtg ttctcgcctt cgtcgcggta tcctttatcg taccgcaacg gaactggggg
    12301 cgacgaagat cgcgttgggt caccatcgtg acgatatcct gcaaacgttg ttcttaaata
    12361 tgttctacgg cggtaagatg aaaggtatgc ctccgaaact gatgagcgat gatggcaaac
    12421 atatcgttat tcgtccgctg gcctactgcc gcgagaaaga tattcagcga tttgccgatg
    12481 caaaagcgtt cccgattatt ccgtgcaacc tgtgcggttc acagcctaac ctgcaacgtc
    12541 aggtgattgc tgacatgttg cgtgactggg ataaacgtta tccagggcgt atcgagacga
    12601 tgttcagcgc gatgcagaat gtggtgccgt cgcatctgtg cgataccaac ctgttcgatt
    12661 tcaaaggcat cacccacggt tctgaagtgg ttaacggggg tgatctggcg tttgatcgcg
    12721 aagagatccc actacaaccg gcgggctggc agccagaaga agatgaaaat cagttggatg
    12781 agttacggct gaatgtggtt gaagtgaaat aaccaggata gcgcccgatg cgcaagcgta
    12841 tcgggctact cttatggagg ccggataaga cgcggccagc gtcgcatccg gcaatcccga
    12901 ataagatgtt tactcttgca cccggcaatt caacatttca ttattttaat aaccgcaccc
    12961 ggcacgtttt tcctttaatc ttcccgccct gtaactgttt ccatgcttta tgagcaacag
    13021 cctgacggac cgcgacatag acatgcgccg gatgcacggc gattttgcca atatctgcgc
    13081 catcaagccc gatatctcct gtcagtgcac ctaatacatc acccgggcgc attttggctt
    13141 ttttcccgcc atcgatacac aacgttgcca tttctgcttc cagcgtcgca atggaactat
    13201 tagctggcgg cgtttgccag ttaagtttta tctgcaacat gtcagaaatg atattggccc
    13261 gctgtgcttc ttccggagca cagaaactga tcgccagacc gctatttcct gcacgagctg
    13321 tacgaccgat gcgatgtaca tgaacttcag ggtcccacgc cagctcaaag ttcaccacca
    13381 gctcaagcga tttaatatcc agaccacgcg cagcaacatc agtcgcgacc agtacacggg
    13441 cgctaccgtt agcaaaacgt accagggtct gatcgcgatc gcgttgctcc aaatcgccgt
    13501 gtaatgacaa tgcactttgc cctacttcat tcagcgcgtc gcagacagcc tggcaatctt
    13561 ttttggtatt gcaaaacacc acgcaagagg atggctgatg caagcttaat aaccgttgca
    13621 acagaggaat tttgcctttg ctggatgtct cataaaattg ttgttcaatg ggtggcaaag
    13681 catctgttga gtcaatttca atcgccaaag gatcgcgttg cactcgtccg ctgattgcag
    13741 cgatggcttc cggccaggtt gccgaaaaca gaagcgtctg tcgagatgca ggcgcaaaac
    13801 ggatgacatc atcaatggca tcgctaaatc ccatatccag catgcggtcg gcctcatcca
    13861 tcaccagcgt attcaacgca tccagtgata ccgtgccttt ttgcaggtga tccagcaaac
    13921 gccccggcgt tgccacgata atatgcggcg catgttgcaa cgaatcacgc tgcataccga
    13981 acggttgacc accgcacaac gtcaaaattt tggtatttgg cagaaaacgc gccagccgac
    14041 gcaattcacc tgccacctga tccgccagtt cacgcgtagg acacagcact aaagcctggg
    14101 tttgaaatag cgacgcatca atttgctgta acaagccgag gccaaaagcc gccgttttgc
    14161 cgctgccggt tttcgcctgc acgcgaacat cttttccggc aaggatcgcc ggaagcgcgg
    14221 cggcctgcac cggcgtcatg gttaaataac ccaactcatt aaggttcgtg agttgggcgg
    14281 gaggcaaaac attcagggta gaaaaagcgg tcacaatcta ttctcgtggt catcgacgca
    14341 aagttagcag gcgcgtatcc tcgcagatct acgctcacga tgcgacaatt taatcggttc
    14401 ttcatcgggt ggtgggtcag gcatgggttg cgggcgaggg atcggatcgg gcactggaac
    14461 aggatcgcca ggaatcggtt cagggacagg aatttgcaaa taaataagtg tcgtcatatt
    14521 tccctctggt cattgggtgg actcttaaag ggtagacgct gataaataac aggcaaaaaa
    14581 aagccgactc atcaaagtcg gcgtcgtacg aatcaattgt gctatgcagt aattcaaaaa
    14641 aggaagtaag acaatatgga gcgcaacgcc catcgcttga cgttgcattc acctgcaaga
    14701 gagatattgc cctgaatggg tagagagttt attgacttcg ctcaaacttt gcggcgtttt
    14761 tgtatacaga cagccggaaa aattgctttt gttacaacca tttactacga tgcaaccata
    14821 aagcaacacc accaataaga acaactaaca gaatacaaaa aattgaaaat ccgaattgcc
    14881 acccgccgcc agggatccca ccaaggttga cgccaaataa cccggtcaga aaggtactgg
    14941 gtaaaaagac cattgccatc aacgacattg tataggtacg acgagctaaa ttttcctgca
    15001 tcacctgagc gatttcatcc gccatcacgc cagtccgtgc tatacaggcg tcgatttcgt
    15061 caaggccgcg cccaaggcga tcggcaatat cctgcatccg acggcgttgg tcatcgctca
    15121 tccacggcaa acgttcactg gcaagacgag cataaacatc acgttgcggt gccatatagc
    15181 gacgcatcac aattaattgt ttgcgcagca gagccaggaa tccacgcggt ggaatttgct
    15241 gatcaaggag attatcttca aggtcgataa ttttatcgtg cagctgctcg ataaattcac
    15301 tggaatgatc ggtcaacgca tcgcacacat ccaccagcca tcccccgcaa tcggtcggac
    15361 ccgtgccctc ttccagatcg ctcaccacat cgtccagcgc cagcactttg cgttgtcggg
    15421 tcgaaacaat taaccgcccg tccatatata cacgcatggc gaccagttga tcggggcgtt
    15481 catcggtgct gccgtttata cagcgcaatg taatcagcgt gccttcaccg agacggctga
    15541 ctcggggacg cgtgctctcg cccgccagcg catcacgtac gttattggga agcagcggtg
    15601 ttgtcgccag ccattgggcg ctatcatggt gtacataatt aaggtggagc caacagggat
    15661 gcgcttcatc aatcacatct gtattttcca gcggtttaac gccgcctcta ccatccagca
    15721 tccaggcaaa tactgcatcc gggacattaa cgtccgatcc cttaatcgct tccacagtgc
    15781 ctccatcatc aacgcattat tttgtagtct agccttctgg ccctgttacg caacatctca
    15841 tcaccccatt accctgaaat gattaataaa attctgtcta aattgaatac aaaaagcaaa
    15901 atgcttttcc gtatacaaac cgtgtgaagt gttaaatagc gtctatcatt atcagaatta
    15961 tctgatcata tgacgtggct tttttgcgat cggatagcaa caaaaattga taaaaataac
    16021 gggatctcaa tgattacgca caacttcaat accctggact tactcaccag tcctgtctgg
    16081 atcgtttcgc cctttgagga acagttaatt tatgccaata gcgcggcgaa actgttgatg
    16141 caagacctca cgtttagtca gctacgaacc ggaccctatt ccgtctcctc acaaaaagaa
    16201 ctgccgaaat acctctccga tctgcaaaac caacacgata ttatcgaaat cctcactgtt
    16261 cagcgtaaag aagaggaaac agcattgagc tgtcggcttg ttttgcgaaa gctgacagaa
    16321 acagaaccgg tgattatttt cgaaggtatc gaagcgccgg caacgctggg tttaaaagcc
    16381 agtcgctcgg caaattatca gcgcaaaaaa caaggttttt atgcgcgctt ttttctgact
    16441 aactctgcac caatgttgtt gattgacccg tcacgagatg gacaaatcgt cgatgctaac
    16501 ctcgccgcgc tcaatttcta tggttataac catgaaacga tgtgccagaa acatacctgg
    16561 gaaataaata tgctcgggcg tcgcgtcatg cctatcatgc atgaaatctc gcatttaccc
    16621 ggtggtcata aatctttgaa ttttgttcat aaactggcgg atggttcgac tcgtcatgtg
    16681 cagacctatg ccggaccgat tgaaatttat ggcgacaagc tcatgttatg tattgtgcat
    16741 gatattactg agcaaaaacg gctggaggag cagctggaac atgctgctca ccatgacgcg
    16801 atgaccggat tactgaatcg gcgacagttt tatcacatta cggaaccagg ccaaatgcag
    16861 catctcgcca tcgctcagga ttacagcttg ttgctcatcg acaccgatcg ttttaaacac
    16921 attaacgatc tctatgggca ttctaaagat gatgaggtgt tatgcgccct cgcccgcacc
    16981 ctcgaaagtt gcgctcgcaa aggcgatttg gtgtttcgtt ggggaggcga agagtttgtc
    17041 ttattgctac caagaacccc actggatacc gcgctttcgc tggctgaaac tatccgcgta
    17101 agcgtggcaa aagtgagtat ttcgggctta ccacgcttta ccgtcagcat tggtgtggcg
    17161 catcacgaag gaaatgaaag catcgatgaa ctgtttaaac gcgttgatga tgctttgtat
    17221 cgggcgaaaa atgatggacg caaccgcgtg ctggcggcat aagccgcgga tgcgtctcga
    17281 gatcaacgac tgcgcttagc gtggcgctcc cagttttctt gcttcgcctg cgccgtttta
    17341 cgtagtgcga cgtaacacgc tccgctgccg ccatgatgcg gtagcgcggt gcaatatgcc
    17401 tgaacatcat caaattcggt cagccagcgc gccacatagc tgcggacaat attggcatgc
    17461 gatttatcat cccgcccttt accatgaata atcagcacgt tacgcaaacc atccgccagg
    17521 gcttgttgaa taaaactgaa caccattttg cggcactctt ccaccggctg gcgcaaaaga
    17581 ttcaggctcg cctgttgcgg atatttacca ctgcgcagct tatccagcac cccatgttgc
    17641 aacccttccc gccgaaactc cagcggctga cttagtggga tgatgtcgag aaatccggtg
    17701 gtgaggaaat tatcaagctg cagcgtgtcg atacgctgcg gcgcacgttg gttacgcgtt
    17761 ggatgccagt ggacatcggt agcacgtttc agcggctgga catcttccat ggcgtcaaga
    17821 aacagcgatt tgtcgtcaag gttcatgtta catcctcccg caattaagag cgcgatatga
    17881 taaccagacc gggtcggtcc aacaacgtat tacccaaatt tccagtaata agttccaaat
    17941 attgccgata ttttaagcaa aatacttat
//
LOCUS       DAJOSY010000087        17698 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJOSY010000087 DAJOSY010000000
VERSION     DAJOSY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.087"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(22..783)
                     /gene="adiY"
                     /locus_tag="OHN27_003190"
     CDS             complement(22..783)
                     /gene="adiY"
                     /locus_tag="OHN27_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCQ2287578.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFVSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(1108..3375)
                     /gene="adiA"
                     /locus_tag="OHN27_003191"
     CDS             complement(1108..3375)
                     /gene="adiA"
                     /locus_tag="OHN27_003191"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCQ2287579.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(3574..4482)
                     /gene="melR"
                     /locus_tag="OHN27_003192"
     CDS             complement(3574..4482)
                     /gene="melR"
                     /locus_tag="OHN27_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCQ2287580.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            4765..6120
                     /gene="melA"
                     /locus_tag="OHN27_003193"
     CDS             4765..6120
                     /gene="melA"
                     /locus_tag="OHN27_003193"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCQ2287581.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKKASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            6223..7644
                     /gene="melB"
                     /locus_tag="OHN27_003194"
     CDS             6223..7644
                     /gene="melB"
                     /locus_tag="OHN27_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCQ2287582.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(7783..8412)
                     /locus_tag="OHN27_003195"
     CDS             complement(7783..8412)
                     /locus_tag="OHN27_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCQ2287583.1"
                     /translation="MTRTLKPLILNTSALTLTLILIYTGISAHDKLTWLMEVTPVIIV
                     VQLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(8535..10181)
                     /gene="fumB"
                     /locus_tag="OHN27_003196"
     CDS             complement(8535..10181)
                     /gene="fumB"
                     /locus_tag="OHN27_003196"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCQ2287584.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(10259..11599)
                     /gene="dcuB"
                     /locus_tag="OHN27_003197"
     CDS             complement(10259..11599)
                     /gene="dcuB"
                     /locus_tag="OHN27_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCQ2287585.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(12170..12889)
                     /gene="dcuR"
                     /locus_tag="OHN27_003198"
     CDS             complement(12170..12889)
                     /gene="dcuR"
                     /locus_tag="OHN27_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCQ2287586.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(12886..14517)
                     /gene="dcuS"
                     /locus_tag="OHN27_003199"
     CDS             complement(12886..14517)
                     /gene="dcuS"
                     /locus_tag="OHN27_003199"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCQ2287587.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPKPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            14698..14928
                     /locus_tag="OHN27_003200"
     CDS             14698..14928
                     /locus_tag="OHN27_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCQ2287588.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            14940..15212
                     /locus_tag="OHN27_003201"
     CDS             14940..15212
                     /locus_tag="OHN27_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2287589.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            15439..15735
                     /gene="ghoS"
                     /locus_tag="OHN27_003202"
     CDS             15439..15735
                     /gene="ghoS"
                     /locus_tag="OHN27_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCQ2287590.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            15763..15936
                     /gene="ghoT"
                     /locus_tag="OHN27_003203"
     CDS             15763..15936
                     /gene="ghoT"
                     /locus_tag="OHN27_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCQ2287591.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(16060..17577)
                     /gene="lysS"
                     /locus_tag="OHN27_003204"
     CDS             complement(16060..17577)
                     /gene="lysS"
                     /locus_tag="OHN27_003204"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCQ2287592.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
BASE COUNT         4373 a         4170 c         4457 g         4698 t
ORIGIN      
        1 gctcttcgtt aaggttaaaa atcaggcgac agtgcgttct ctgtgctgac taacataatg
       61 cagcggcgtc ataccgtaga agtctttaaa gacagaaata aagtacgacg tactgttgta
      121 gccgcaggac tgtgatacct gagagatatt tttaccgtcc atcattaatt catttacggc
      181 ataacgcatg cggcaggtgg tgattatttg gctataactg gtgttttcgc ttttcaactt
      241 tttctttaac agacttgggc taagacataa acaactggct accatactaa gattccagtc
      301 tttgtgaata tcgctttcaa taatttgata aacgctgtca cttacacaat tacgtaacat
      361 atacatcatt aatgaaacga attttttact gtcgagaaaa cgggatagca ccgtgaataa
      421 taatgcgcgt gttcgttcct tttctgactc agtttctgcg ggcatcatgc tgtgttgcgc
      481 ggcaacgcga aagacgtctg gcgtcaggca tggcagggtg agtatgggcg tagcgctacg
      541 ttgccatact ggtatttgcg agagatcttt attcagaaac cggaggtaat ctttgatgat
      601 gtcgtgacta atatgagcaa ccaaagtgtt gttaagagag gagacgtcga taatattatt
      661 ttcacaattt aataacgcca tatggttagc tttaaggcta ataggttctt tcccattcac
      721 ccttatccag acatcttttt cagtcaataa aacaatacaa ggttggtcgc tgcaaatcct
      781 catacatgta ctcctgagtg cgaataaaag accagttctc gctaaagcaa agcgataccg
      841 ggaaatggtt atataattgt atgatgaata taaacatgca taaaaataac gagttttact
      901 aatagtacaa atttttatag taatttctca tgtacatatt tttttacagg ataaataaca
      961 ggcaaaaaaa agcgcggggt ggggccgcgc aaaaagtatg acatttttgt ttgcagcaat
     1021 ttttatgaaa acgttgccat actcaaaagg cctaaggcca ggcggcgtaa tgttatttaa
     1081 acaattacgc cttcagcgga atagtggtta cgctttcacg cacataacgt ggtaaatacc
     1141 gtcaataatt tcagtccctt cagtttcgtg ttcaaatcca gggaaatggt ggtcccagga
     1201 ttgcagcgag cgtaaataac ttacttgcgg actgttttta tcgccgaagt tttcaccaga
     1261 cagcagcatc gggattcctg gcggatacgg gataactgag tttgccgcga tgcgtcctgg
     1321 cagattttca atggatacca gttcgacatt gttgtcgaca atcgcgttgt acgcttcacg
     1381 cggggtgact tccgccaccg gcaggccgga ataggcttcg ttcaaccgtg cgccagggtt
     1441 gttttctttc agccaggcaa acatggtgtc acccagatcg tgaatcccca tgttcgcgta
     1501 agtgtcagga tattgttcaa caagttccgg catcacctgc gccagcggtg tgttggcgtc
     1561 atagtggcgt ttgaaggagc aaagggtgtt aaccagagtt ccccatttcc cacgggttac
     1621 gcccatagag aacaggaaca taatttggaa gtcagtggtg cgggtaggta caatgccgtg
     1681 gcgaccaagc caggcagtga ccagcgccgc cggaacaccg gtttcttcca gttcaccatc
     1741 ttcacccatt cccggagcaa ggatgctgac tttaatcggg tcgagcatac tccagttatc
     1801 cggaatatct ttgaagccgt gccagctttc gcccggatgc attacccagc agtcctgaac
     1861 ggtggtcagc agtttggttg gtgcgtcagc aaagtcatag gttttgccgg tttgtgggtc
     1921 ggtgacgact tctttgttcc acggtttgaa gaaccagcta ccgtcagcgg tgaactcttt
     1981 atatagccgc gccatcgcct gacggaaatc aaccgcttcg tcaatcactt cctgtgtcag
     2041 tgacaggccg ctgttgccgt ccatcatcga caccgccacg tcgttggatg cgcagatggc
     2101 atacagcggg gaggtggtgg catgcatcat gtaggcctgg ttgaagcggg agaagttaat
     2161 cgccccacga ccttcacgta catgaatata agaagcctgt gacagcgcat tcagcagttt
     2221 gtgggtggag tgggtggcga aaacggtagg accgttgtga tcgccaggtt cgccgcgcat
     2281 ggcatagtga tcggcataga tcgggttgaa acgtgcatag ccgtaccagg cttcgtcaaa
     2341 gtgcagacga tcggaggttt tttccagcag atcctgcgct tctttagcgt tataacacac
     2401 gccgtcatag gtgcagttgg tcaccacgca gtaagacggt ttttgcccgg ctttgtcttt
     2461 ggtcagcggg ctttcactga ttttcttctg caaggtttca ggttgcattt cctgcggata
     2521 gattggcccg ataatgccgt agcggttgcg gcttggcacc atatagaccg gtttcgcgcc
     2581 tgtcagcatc aaaccttgtt cgatggattt atggcagtta cggtcaacga ccacgacatc
     2641 gttatcggtc atgcaagcct gcatgatggt gcggttagag ccggaagtac cgacgactac
     2701 cgaccaggag cgatcggcac caaatacgcg tgcggcatat ttttcgcttt cgccaaatgc
     2761 gccagtatgg tcaagcaaag aaccgaggga agttcgttcg atgcccatgt cggtgcggaa
     2821 cagattttca ccatagtagt catggtagaa acgtccggcg ggtgttttgg taaaaccaac
     2881 gccgccctgg tggcctggcg ctgcccagga atattcatgg atgtcactat atttcatcag
     2941 cgcgctgaac agtggcggca acagctgctg gcggtagcgg gtcatcgcgg caacggcgcg
     3001 tccggcgata aagtcggcgg tatcttccag aatccaggcg aattcatcga caagctccag
     3061 caggtcgcga tccattgcgg cgagggcttt ttcccgatcg cccaacagga agaccggcac
     3121 gttttgttgg cgctcatgaa gcttaccgat caattgtctg acgttttgat gttcgtccgg
     3181 atgttccatt tgatagctga acatcaggca gtcaatggct tcgtttgaag agagaatggc
     3241 aaaaccatca tcaaaggagg tggatttaat cacggtaaca ttttgctggc ttaaagcatc
     3301 tgccagacgc tcaacggcgt taccgaccca ggtgtcttga tggagaaact cgctttcaac
     3361 aattaatact ttcatcattg cttacccggt tatgaaggaa tcttcgttgc gggcaagtat
     3421 cttccggcct tttcgggctg taaagcgcgt gaaaaacaaa catttttgaa atatttagtt
     3481 ttataaacaa tacgttatgt gattatttta aattattttc actgcttatt atcgacggct
     3541 aaactatttt tttggctgat actgatatcg tctttagccg ggaaacgtct ggcggcgctg
     3601 ttggctaagt ttgcggtatt gttgcggcga catgccgaca tatttgccga acgtgctgta
     3661 aaaacgacta cttgaacgaa agcctgccgt cagggcaata tcgagaatac ttttatcggt
     3721 atcgctcagt aacgcgcgaa cgtggttgat gcgcatcgcg gtaatgtact gtttcatcgt
     3781 caattgcatg acccgctgga atatccccat tgcatagttg gcgttaagtt tgacgtgctc
     3841 agccacatcg ttgatggtca gcgcctgatc atagttttcg gcaataaagc ccagcatctg
     3901 gctaacataa aattgcgcat ggcgcgagac gctgtttttg tgtgtgcgcg aggttttatt
     3961 gaccagaatc ggttcccagc cagagaggct aaatcgcttg agcatcaggc caatttcatc
     4021 aatggcgagc tggcgaattt gctcgttcgg actgtttaat tcctgctgcc agcggcgcac
     4081 ttcaaacggg ctaagttgct gtgtggccag tgatttgatc accatgccgt gagtgacgtg
     4141 gttaatcagg tctttatcca gcggccagga gagaaacaga tgcatcggca gattaaaaat
     4201 cgccatgctc tgacaggttc cggtatctgt tagttggtgc ggtgtacagg cccagaacag
     4261 cgtgatatga ccctgattga tattcacttt ttcattgttg atcaggtatt ccacatcgcc
     4321 atcgaaaggc acattcactt cgacctgacc atgccagtgg ctggtgggca tgatatgcgg
     4381 tgcgcgaaac tcaatctcca tccgctggta ttccgaatac agcgacagcg ggctgcgggt
     4441 ctgtttttcg tcgctgctgc acataaacgt atctgtattc atggatggct ctctttcctg
     4501 gaatatcaga attatggcag gagtgaggga ggatgactgc gagtgggagc acggttttca
     4561 ccctcttccc agaggggcga ggggactctc cgagtatcat gaggccgaaa actctgcttt
     4621 tcaggtaatt tattcccata aactcagatt tactgctgct tcacgcagga tctgagttta
     4681 tgggaatgct caacctggaa gccggaggtt ttctgcagat tcgcctgcca tgatgaagtt
     4741 attcaagcaa gccaggagat ctgcatgatg tctgcaccca aaattacatt tatcggcgct
     4801 ggttcgacga ttttcgttaa aaatattctt ggtgatgtgt tccatcgcga ggcgctgaaa
     4861 acggcgcata ttgccctgat ggacattgac cccacccgcc tggaagagtc gcatattgtg
     4921 gtgcgtaagc tgatggattc agcaggggcc agcggcaaaa tcacctgcca cacccaacag
     4981 aaagaagcct tagaggatgc cgattttgtc gtggtggcat ttcagattgg cggttatgaa
     5041 ccttgcacgg tgactgattt cgaggtctgt aagcggcatg gtctggaaca aaccattgcc
     5101 gatacgttgg ggccgggcgg tattatgcgc gcgctacgta ccattccgca tctgtggcaa
     5161 atttgcgagg acatgacgga agtctgcccc gatgccacca tgctcaacta tgttaaccca
     5221 atggcgatga atacctgggc gatgtatgcc cgctatccgc atatcaaaca ggtcgggctg
     5281 tgccattcgg tgcagggaac ggcggaagag ttggcgcgtg acctcaatat cgacccagct
     5341 acgctgcgtt accgttgcgc aggtatcaac catatggcgt tttacctgga gctggagcgc
     5401 aaaaccgccg acggcagtta tgtgaatctc tacccggaac tgctggcggc ttatgaagca
     5461 gggcaggcac cgaagccgaa tattcatggc aatactcgct gccagaatat tgtgcgctac
     5521 gaaatgttca aaaagctggg ctatttcgtc acggaatcgt cagaacattt tgctgagtac
     5581 acaccgtggt ttattaagcc aggtcgtgag gatttgattg agcgttataa agtaccgctg
     5641 gatgagtacc cgaaacgctg cgtcgagcag ctggcgaact ggcataaaga gctggaggag
     5701 tataaaaaag cctcccggat tgatattaaa ccgtcacggg aatatgccag cacaatcatg
     5761 aacgctatct ggactggcga gccgagtgtg atttacggca acgtccgtaa cgatggtttg
     5821 attgataacc tgccacaagg atgttgcgtg gaagtagcct gtctggttga tgctaatggc
     5881 attcagccga ccaaagtcgg tacgctacct tcgcatctgg ccgccctgat gcaaaccaac
     5941 atcaacgtac agacgctgct gaccgaagct attcttacgg aaaatcgcga ccgtgtttac
     6001 cacgccgcga tgatggaccc gcatactgcc gccgtgctgg gcattgacga aatatatgct
     6061 cttgttgacg acctgattgc cgcccacggc gactggctgc caggctggtt gcaccgttaa
     6121 aacgcgacta aacgctactg cgccggggga tttattccgg cgcacacctc tgacgatacc
     6181 aataacagaa ggcgggcgtt ggtaacagcg acccgatacc ctatgagcat ttcaatgact
     6241 acaaaactca gttatggatt tggagcgttc gggaaggatt ttgcgatcgg cattgtgtat
     6301 atgtacctca tgtattacta caccgatgtc gtcgggctgt ctgtgggttt ggtcggtact
     6361 ttgtttctgg tggcgaggat ctgggatgct attaacgatc cgattatggg atggattgta
     6421 aatgctacgc gatcgcgatg gggtaagttc aaaccctgga tcctgatcgg tacgttggca
     6481 aactctgtaa tcttatttct cctctttagt gcgcatctgt ttgaaggtac tactcagatt
     6541 gtctttgttt gcgtgaccta catcctctgg ggcatgactt acaccattat ggatattccc
     6601 ttctggtcgc tggttccaac catcacgctc gataaacgtg agcgcgaaca actggttcct
     6661 tatccgcgtt tttttgccag tctggcaggc tttgttacgg caggtgtgac gctaccattt
     6721 gttaattatg tcggcggtgg cgatcgggga tttggctttc agatgttcac tctggtactg
     6781 atcgcctttt ttattgtttc aaccatcatc actctgcgca atgtgcatga agtcttttcg
     6841 tcagacaatc aaccgtctgc tgaaggaagc catctgacac ttaaagccat cgttgcgcta
     6901 atttataaaa acgatcagct ttcatgcctc ttgggtatgg ctcttgctta taatgtagcc
     6961 agcaacatta ttaccggctt tgctatctat tatttctcat atgttatcgg tgatgcggat
     7021 ttgttcccct attatctgtc gtatgcggga gctgctaacc tggtgacgtt agtattcttc
     7081 ccacgcttag ttaaatcatt atcccgacgc attttatggg ccggagcatc tattcttccg
     7141 gtgttaagct gtggtgttct cctgttaatg gcattaatga gctatcacaa cgtcgtcctc
     7201 attgtgattg cgggtatttt gctgaatgtg ggaacggcgc ttttctgggt attacaggtc
     7261 atcatggtgg cagataccgt tgattacggt gaatataaac tgcacgtacg ctgtgaaagt
     7321 atcgcttact ccgtgcagac tatggtggtg aagggcggtt cagcctttgc ggcttttttc
     7381 attgcggttg tgttagggat gattggctat gtaccgaatg ttgaacagtc tacgcaagcc
     7441 ctattaggta tgcagtttat tatgattgct ctaccaactc tgtttttcat ggtaacgctg
     7501 attctctact tccgtttcta tcgcctcaat ggtgacacgc tgcgcaggat ccagatccat
     7561 ctgctggata aatatcgcaa agtaccgccc gagcctgttc atgctgatat tccggtcggt
     7621 gcagtgagtg atgtgaaagc ctgacgtgga caatagctaa cgaaaatggc ctgatgcgat
     7681 atgtttatca ggccacgtct taatgtaatg ctttggattt gcaaattttg tagaccggat
     7741 aaggaattca cgccgcatcc ggcatcaaca aagcgcaagt tgttatccgg ttatcaagcc
     7801 aaagcgccgt agctggcggc aatgaaagcg agcgaggaat atcaccgtcg ttaatgcgcc
     7861 aagcagcgcg cagaacatat cggattgcgt gtcccactgg tcgccctggg tgccgagaaa
     7921 atcatccgct ccctgaccca ttgccagcgc tgcccaccac tcgattaatt catacatcgc
     7981 gcttatcgcc agcgccacgc agcagaccag gaacgccacc attttacgtc cgcgcacgta
     8041 catcccgcga acgagaattt ctcgtgccac cagtgcaggc accagcccct ggaaaaagtg
     8101 ccccagcttg tcatacggat tacgactcaa ccccagccat tcctgcacct caaaaccaac
     8161 ggggactttc gcgtaggtgt attgtccgcc gaccatcagg atgatggcgt gaaggaaaat
     8221 gagcgtatag agcagcggcg ttaacggata acgtctggcg gtggcaagca gtagctgcac
     8281 gacaataatc accggtgtca cttccatcag ccaggtgagt ttgtcatggg ccgaaatgcc
     8341 ggtataaatc aggattaacg ttagcgtcag tgcgctggtg ttaagaatta acggcttgag
     8401 tgtgcgggtc atggtgagtt cgcaaatcag ggaaaatact gactattcac cagcaagcgt
     8461 gaaattacaa tcgcaaaaga ggtggcccag ggggatcacc tggcagcatg ctgccaggcg
     8521 ctgggccgaa gaggttactt agtgcagttc gcgcactgtt tgttgacgat ttgctggaag
     8581 aagtcgttac ctttgtcatc gaccaggata aacgccggga aatcttctac ttcgattttc
     8641 cagatagctt ccatacccag ctccggataa gcgacgcact ccagatgctt gatgctctgc
     8701 tgcgccagta ccgccgccgg accgccgatg ctaccgaggt agaagccgcc gtgtttatga
     8761 cacgcgtcgg taacctgctg actgcggtta cctttcgcca gcatgatcat gctgccgccg
     8821 tgggattgca gcagatccac gtaggagtcc atacggcctg cggtggttgg gccaagtgaa
     8881 cctgatggat aaccggcagg ggttttcgcc ggacccgcgt agtagatcgg gtgatctttg
     8941 atgtactgcg gaagttcttt accggcgtca atcagctctt tcagcttggc gtgtgcaata
     9001 tctcggccca cgataatggt gccggtgagc gacaaacgag tggataccgg gtattgcgaa
     9061 agctgggcga ggatctcttt catcgggcgg ttaaggtcaa ctttcaccgc ttcgccttca
     9121 ccggcctggc gcagttcttg tggaatgtac tggcctgggt tgtgttccag tttttcgatc
     9181 cagatacctt cgcggttgat tttcgcttta atgttacggt cagcggagca ggagacgccc
     9241 atgccgaccg ggcaggatgc gccgtgacgt ggcagacgga taacgcgaat gtcgtgcgcg
     9301 aagtatttac cgccaaactg cgcgccaaga ccgagtttct gggcctcttc cagcagttcc
     9361 tgttccagct ggacatcgcg gaacgcctga ccatgttcgt tcccttccgt cggcagttca
     9421 tcgtaatagt gagcgcttgc taacttgacg gttttcaggt tggtttccgc agacgtaccg
     9481 ccaatcacaa acgcgatatg gtacggcggg caggctgcag taccgagggt acgcattttc
     9541 tcgacgagga agtttttcag tttgccggga gtcagcaggg ctttggtttc ctggtagaga
     9601 tacgttttgt tggcagagcc gccgcctttc gcaacgcaaa ggaatttgta ctcatcgcca
     9661 tctaccgcgt acaggtcgat ttgcgcaggc aggttagtgc cggtgttgac ctctttgtac
     9721 atgtccagcg ccgcattctg tgaatagcgc aggttatctt cgatataggt gttatagacg
     9781 cctttcgaca gcgcttcttc atcaccgccg ccggtccaca cgcgctggcc ttttttaccg
     9841 acgatgatcg cggtgccggt atcctggcag gtcggcagca cgcctttggc ggcgatttcg
     9901 gagtttctta agaattgcag cgccacgtac ttgtcgtttt cgctggcttc tggatcgtga
     9961 agaatagccg caacctgttt ctggtgtgcc gggcggagca taaaagaagc gtcgtgaaag
    10021 gcttgctgcg ccagcagggt cagggcttct ggttccactt tcaggatggt ttcgccgtcg
    10081 aagtcggcaa cgctaacgta atcggaagtg agtagatagt attcggtatt gtctttcccc
    10141 atcgggaaag gtgcctggta gataaagggt ttgtttgaca tagcttccag cctgtaactc
    10201 tgtatttgtt attcgaaaat ggcgtgccgc gtagtcacgg gcggcacgca aagtgcattt
    10261 ataagaaccc gtacatcgcg gcgaagatcc agccgaagac gcacgatacg ctcacaccaa
    10321 tcaaccccgg cagaataaag ctgtggttga tgacgaagcg accgatgtgg gtggtgccgg
    10381 aacggtcaaa ctgaatcgct gccagatcgc tcggataagt cggcaggatg taataaccgt
    10441 agcaagccgg tgctgaagcc acgatgtatg ccggatcaac gccgatcgcc agcgcgaccg
    10501 gaacaatcgc cgccagcgcc gcagcctgag agtttacaaa cttggaaacc agcagcagaa
    10561 caatggcata ggcccacgga tactctttca ccatttcacc cagtacgccc tgaatttcag
    10621 acatatgcgc accgaacatg gtttctgcca tccatgcgat accgtacacc gccacgatgg
    10681 cgatcatacc ggaacggaag acttcgtttt ttgagataga cgcgggattg gttttggtca
    10741 ggataataat cagcgccccg gtcagcagca taaacatctg aataaccagc accatcgaca
    10801 gcggtttgcc gccgaaggat ggacgcaggt ccgaatcagc accaagaagt gcgactacag
    10861 cgattgcccc gaggaaaatc cacatcgcca gccagttgct tttcggcagt tttttatcca
    10921 gcagcgtcgc ggtatcaccg taaacatact cacggttttc cggtacggag atgaatttct
    10981 ggaactcttc gtctttatcc agatctttac cgcggaacca gctgaagata ccgatcgcca
    11041 ggataccgat taacgtcgat ggaatggtga ttgccagcag atcgaggaac tcaagatggc
    11101 ggccatcaaa ggtgacatta cccagcatcg caaccagaga cacgaccgca accgataccg
    11161 gactggcgat aatccccatc tgtgcaccga tagaacttgc cgccatcgga cgttccggac
    11221 ggatgttgtt cttaatggcg acgtcgtaga tgatcggcag aatggtgtaa accacatgac
    11281 ccgtaccgca aagaatggtc agtgtacagg tcacaaacgg cgcgacaatt gagacatatt
    11341 tcgggttgcg gcgcagcagc ttctcggcaa tttgcagcat gacatcaaga ccgcccgaag
    11401 cttgcaaggt cgccgatgcc gccaccaccg caatgataac cagcatgaca tcaactggtg
    11461 gtttacctgg ctgaaggtgg aagacgaaga ccagaatgac cagaccgata ccgcctaata
    11521 aacccagcgc gataccaccc tttctggcac cataaaacag acatatcagt attatgataa
    11581 gttggatagt aaataacatg tgtgaaccct cgcgataatc ctatttaaat ttttgctgaa
    11641 tagatcacag tcacgttctg ttttgtatga actgttttca gagcctgact aaatatccgt
    11701 ttggtcgtta ccggctttag caaatacctc acagtgaata ttggctgatt aaagcagcgg
    11761 caaatttatg cactgtgata agcggctttt tcagagagaa agactctctg gcgatgggtt
    11821 tatagtttgt actgcgcaga tgttaatgtt ttgtttgctg ttatcttatt gatatttatc
    11881 ggtctatttt ttgttctcag ggcagatgtc attaggttta tagattaatc tgatctaccc
    11941 atttgtgggt aaaaatacac ataatgcggg tgacataata gttaattaac ttttgttagc
    12001 gttttgaaat taaaaacacc gttcacctga agagatatta atttttagcg atgatggagg
    12061 gataattata tttgatctgg cacaagtttt actgatgaag gatgtaactt gtgccagggg
    12121 tattttgcat tacggtaata attattactt acagataacc agcgatccgt tattggcaat
    12181 attgtttcag tagtgagtag tgttctgcct gaatacggta acggtaaacc ggacgccccg
    12241 tgacgccata atggatactg gtgaacaaga tgtggcagtt gaccagccag atgaggtatt
    12301 tacggcagga aacacgcgag atgttaacct cgttagccag ctcgtcggtt gaaaactcat
    12361 agtcctgatg cgcgtcaatc cactggcaca gcgtgcgtaa cgtctgcggc gttaagcctt
    12421 ttggcaagcg acgaggatcc tgttcgttgg agctgctgcc gtggattagc tgatcaagct
    12481 cggcctggtc ataatactga tgtttttcca gcgccatttt cttttgccgc cagccggtga
    12541 gcgcctcttc aaagcgggaa gcctggaagg gtttgatcag gtaatccacg acaccgtaat
    12601 gcagcgaatc tttaatggtt gccgcatcgg ctgcggagga gatgacaatc acatcacttt
    12661 tgcaacgcgc gttatgcagg acaggcagta aatcgagccc gttctctttt tgcatataga
    12721 tatcgagcaa tatcaggtcg ataggcgtat cgctattgaa gataatctct ttggctttct
    12781 ccagcgtcga ggctgttcca cagcattgaa agcctgggat ttgtgctacg tatcggcgat
    12841 tcagctccgc gaccattgcg tcgtcatcga taattaatac attgatcatc tgttcgacct
    12901 ctccccgtcc cagggtatct ggacaaaaaa ttgtgtgaaa atcccgggtt ccgattccac
    12961 ggcgatgctg ccgccgagat tttctacctg ttgtttgaca agtgctaaac cgacgcctcg
    13021 ctcgcttcct tttgtcgaga cacctttgtc aaaaatgtga tcgattttgt cgggtgcgat
    13081 tcccggtcca tcatcgttaa cttcgcagtg cagccagccg tgacggtagt gcaatgttac
    13141 gctaatttcg ccaccgggtt tcggccctaa tgcttccagc gcgttttcta tcagatttcc
    13201 caatgtggta atcagcgtcg cgacctggtc ctcactgccg ctgtctggca gcaggctttc
    13261 actgtttaaa atcagcgtat ggcctaaatc ggtcgcgcgg ttaatcttgc tgattaaaaa
    13321 accagcgata accggagatt tgatcttacc cagcagagag ccaatctctt cctgatagtt
    13381 attggctgtt ttgagaatgt aatcttccaa ctgcttataa ctcttcagat gcaataatcc
    13441 gagaatcaca tgcaatttat tcataaattc gtgggatcgt tcacgaagtg catcagcata
    13501 gttgaccaga ccgtcgagtc gctgcatcag tttacgaact tcagttttgt ccctgaaggt
    13561 tgaaatggca ccgatgataa cgccattact gcgcaccgga acggtgttga tcagtaatag
    13621 ccggtcttta atcgtaatct cttcgtcgcg gcgcggcgta ccgtcgcgta acacttccga
    13681 gacatctacc acctgtgacc atgagtggct tagcgtcgac agtttctcat cgtcctgcga
    13741 cttacggtaa ttcagcaatt cttgtgcggc atcgttgatc agcgtgacct cgccgcgatc
    13801 gtccacggca acgacgcctt ctttgataga ctgcaacatg gcctggcgct gctcaaacag
    13861 cgtggagatt tcgtagggtt ccaggccgaa aaggattttt ttcagtacct taaccagaat
    13921 gcaggtgcca atcagtccga ccagcatgcc aaataatacc gaccagataa tgctccagcg
    13981 actgtcattg atttgttggg tcacgcggct taactcaagg ccgatcgcca ccacgccaat
    14041 ttgtttatga ttttcatcgt agatgggggt aaatacgcgt aaagcctgcg ccagaaaacc
    14101 gcgattgata gcgacatttt ctttgccatt cagcgcttta aggatgtcat cacctttaaa
    14161 tggctgacca atacgctggg cttcaggatg cgagtagcga agactttgca tatcggtaac
    14221 gacaataaac agcagatcgt tgcgtttgcg cacggcttcc gcgatggcct ggattccgct
    14281 ctcctgcggt tttttctgca agccctgacg gatttccggc gagtcggcga gggtacgcgc
    14341 cactgccagt gccttgttgg ccagcccgtc tcgcgtcata tcactgattt gcgagaagta
    14401 aatcagatgc accaccaata gcaccgagaa cagtaccgcg ctgaccatta agatcactgt
    14461 ggtactcaat ttcatcggac gtttgcgtaa catgcggtag ggcaatgaat gtctcatcag
    14521 cttccttgtg tgacaaattt cttaagcatt atctctgatg aggcgggtaa ttcaaaggga
    14581 gtaagaatga ttggctatat aggggaagag actctggcaa cggaaactgc cagtgctgta
    14641 tgaagattcc agggctatgc ttatagcgat aatcatactg atgagagagg gaaagtcatg
    14701 gatcaggcgc tactggacga gggttatcgc tgttataccg gcgaaaagat cgatgtctat
    14761 ttcaacactg cgatatgtca gcattctggc aattgcgtac gtggcaacgg caagttattt
    14821 aatctcaaac gaaagccgtg gatcatgccg gatgaagtcg acgtcgtcac tgtggttaaa
    14881 gtgattgata cgtgcccgag cggcgcgctg aaataccgtc ataaataagc gagggtaaaa
    14941 tggaaatacg cgaaggccac aataaatttt acattaatga cgaacaaggc aagcaaatcg
    15001 ctgaaattgt ttttgtgccg accggagaga atttagcgat tatcgaacat accgatgtcg
    15061 atgaaagcct gaaagggcaa gggattggta aacagctggt tgcgaaagtc gtggaaaaaa
    15121 tgcgtcggga aaaacgaaaa attatcccac tatgcccatt tgcgaaacac gaatttgata
    15181 aaacgcggga gtatgatgat attcgcagtt gatgggagag tacagattca cgatattttt
    15241 catgctctcc gcggtgtgat gcaggagagc atctgaaagg tagggggatg cacaaagaat
    15301 gggcagagag cgcgtttttt tgtcccaagt catccccttt actgagcaaa aaaaagaata
    15361 tctcctatat gagaatcatc aatcggggtt aataagtttt gcgtcccctg agcgttcaat
    15421 attgatagga gtcatattat ggaaggtaaa aacaagttca atacttatgt tgtttctttt
    15481 gattatccat catcttattc ctcagtgttc ttaagattaa gatcattgat gtatgatatg
    15541 aatttctctt ctatcgtggc tgatgaatat gggataccac gacaattgaa tgaaaattcc
    15601 ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag atttaatcag gcttaaatgc
    15661 ttagacttac cggatattga ttttgacctc aacattatga cagttgatga ctatttccgt
    15721 cagttttaca agtagccagg aaggataaga agaacataaa aaatggcact attctctaaa
    15781 atattaattt tttatgtgat tggtgtgaac atatcctttg tcattatctg gtttatctca
    15841 catgagaaaa cacatattcg tttacttagt gcattcctgg tcggaataac ctggccaatg
    15901 agtctgcctg tggcattact tttttctctc ttttaggagc tgtagttgag tggacccgct
    15961 ggttatcgtg aaacgaagcg tctctcctca aaactggatt tgcggaacga tgaacgaagc
    16021 agtcgcctga ctgcttcgtt cattaaagaa aagagaaatt tatttctgtg ggcgcatcgc
    16081 cgggaagaga ataacgtcac gaatagtatg actgtttgta aacagcataa tcattcggtc
    16141 gatgccaata cccagaccag cggttggcgg cagaccatat tccagcgcag tcacgtaatc
    16201 ttcgtcatag aacatggctt cgtcgtcacc tgcagcttta gcgttaacct gttcctggaa
    16261 acgttcagcc tgatcttctg cgtcgtttaa ttcgctaaaa ccattaccga tttcacgacc
    16321 accgatgaag aattcaaaac ggtcggtgat ttccgggtta acatcattac ggcgcgccag
    16381 cggggacact tctgccggat attccgtaat aaaggttggc tgaatcagat gtgcttctgc
    16441 cacttcatca aagatctccg tgacaatacg tcccagcccc cagcttttct ctaccgtaat
    16501 tccgatagat tcagctaatg ctttagcagc atcaaaatta tccaggtcgg ccatatcggt
    16561 ttctggacga tattttttga ttgcttcgcg catggtgagt ttttcaaacg gtttgccgaa
    16621 atcaaacaca tgctcgccat aagtgacttt agtggtaccc agaacctctt gtgccagggt
    16681 gcggaacagt gactctgtca gttcaatcaa atcgtggtaa tccgcatacg ccatgtagag
    16741 ttccatcatt gtgaactcag gattatggcg aacagaaata ccttcattac ggaagttacg
    16801 gttgatttcg aatacccgtt caaaaccgcc tacaaccaga cgtttcagat acagctccgg
    16861 cgcgatacgc agatacatat ctaaatccag agcattatga tgggtaataa atgggcgagc
    16921 agatgcccca cctggaatta cctgcatcat cggggtttct acttccataa agccgcgcgc
    16981 gaccatgaac tgacggatag cggccagaat ttttgaacgg acaacaaacg tttgacggga
    17041 tttatcgtta gcgatgaggt ccagataacg ttgacgataa cggacttcct gatcctgcag
    17101 accatggaat ttatctggta aaggacgtag tgcttttgtc agcaggcgca actcagtaca
    17161 gtgaatggaa agctcacccg tttgcgtctt aaacagcgta ccgcgggcac cgataatgtc
    17221 acccagatcc cattttttaa actgatcgtt ataaacacct tctggcaggc tatctcgtgc
    17281 gacgtacagt tgaatacggc caccgacatc ctgcaacgtt acaaaggagg ctttccccat
    17341 gatacgacgg gtcatcattc ggccagcaac cgagacttca atgtttaagg attccagttc
    17401 ctggttatcc ttcgcatcaa actcttcgtg caactggtca gaggtatggt cgcggcgaaa
    17461 atcattggga aacgccacac cttgctgacg tagtgccgcc agtttttcgc ggcgatttct
    17521 cagttcatcg ttaaaatcaa tagcctcatt ggctccccgt gtttcttgtt cagacatttt
    17581 ggttcctcta aatccagctt tcaatttagc gtagataaag agacagatcg gtacactgta
    17641 ttatctgcct cgactattag taactcagtt tatcttatcg aaactaacgc ttacctca
//
LOCUS       DAJOSY010000088        17665 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJOSY010000088 DAJOSY010000000
VERSION     DAJOSY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.088"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            80..1219
                     /gene="arnB"
                     /locus_tag="OHN27_003205"
     CDS             80..1219
                     /gene="arnB"
                     /locus_tag="OHN27_003205"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCQ2287593.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALKIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQCCGISRNALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1223..2191
                     /gene="arnC"
                     /locus_tag="OHN27_003206"
     CDS             1223..2191
                     /gene="arnC"
                     /locus_tag="OHN27_003206"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCQ2287594.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2191..4173
                     /gene="arnA"
                     /locus_tag="OHN27_003207"
     CDS             2191..4173
                     /gene="arnA"
                     /locus_tag="OHN27_003207"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCQ2287595.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4170..5060
                     /locus_tag="OHN27_003208"
     CDS             4170..5060
                     /locus_tag="OHN27_003208"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCQ2287596.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5060..6712
                     /gene="arnT"
                     /locus_tag="OHN27_003209"
     CDS             5060..6712
                     /gene="arnT"
                     /locus_tag="OHN27_003209"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCQ2287597.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPNFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6709..7044
                     /gene="arnE"
                     /locus_tag="OHN27_003210"
     CDS             6709..7044
                     /gene="arnE"
                     /locus_tag="OHN27_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCQ2287598.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7044..7430
                     /gene="arnF"
                     /locus_tag="OHN27_003211"
     CDS             7044..7430
                     /gene="arnF"
                     /locus_tag="OHN27_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCQ2287599.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(7424..7690)
                     /gene="pmrD"
                     /locus_tag="OHN27_003212"
     CDS             complement(7424..7690)
                     /gene="pmrD"
                     /locus_tag="OHN27_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCQ2287600.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7800..9155)
                     /gene="menE"
                     /locus_tag="OHN27_003213"
     CDS             complement(7800..9155)
                     /gene="menE"
                     /locus_tag="OHN27_003213"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCQ2287601.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9152..10114)
                     /gene="menC"
                     /locus_tag="OHN27_003214"
     CDS             complement(9152..10114)
                     /gene="menC"
                     /locus_tag="OHN27_003214"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCQ2287602.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10114..10971)
                     /gene="menB"
                     /locus_tag="OHN27_003215"
     CDS             complement(10114..10971)
                     /gene="menB"
                     /locus_tag="OHN27_003215"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCQ2287603.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10986..11744)
                     /gene="menH"
                     /locus_tag="OHN27_003216"
     CDS             complement(10986..11744)
                     /gene="menH"
                     /locus_tag="OHN27_003216"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCQ2287604.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11741..13411)
                     /gene="menD"
                     /locus_tag="OHN27_003217"
     CDS             complement(11741..13411)
                     /gene="menD"
                     /locus_tag="OHN27_003217"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCQ2287605.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13500..14795)
                     /gene="menF"
                     /locus_tag="OHN27_003218"
     CDS             complement(13500..14795)
                     /gene="menF"
                     /locus_tag="OHN27_003218"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCQ2287606.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14874..15179)
                     /gene="elaB"
                     /locus_tag="OHN27_003219"
     CDS             complement(14874..15179)
                     /gene="elaB"
                     /locus_tag="OHN27_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCQ2287607.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15234..15695)
                     /locus_tag="OHN27_003220"
     CDS             complement(15234..15695)
                     /locus_tag="OHN27_003220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2287608.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15760..16677
                     /gene="rbn"
                     /locus_tag="OHN27_003221"
     CDS             15760..16677
                     /gene="rbn"
                     /locus_tag="OHN27_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCQ2287609.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16865..17665
                     /locus_tag="OHN27_003222"
                     /pseudo
     CDS             16865..17665
                     /locus_tag="OHN27_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         4182 a         4741 c         4440 g         4302 t
ORIGIN      
        1 gctccaaatg gcaaaatata cacaacactc tttatagcaa atataagtgg acaggtattc
       61 aatggcggaa ggaaaagcaa tgtcagaatt tttgcctttt tcgcgaccag caatgggcgt
      121 ggaggaactc gctgcagtta aagaggttct cgaatccggt tggatcacaa ccggtccgaa
      181 gaatcaggcg cttgagcaag ccttttgcca gttgacggga aatcagcatg ccatcgcggt
      241 cagttcagcc accgccggaa tgcatatcac gctaatggcg ttgaaaattg gcaagggcga
      301 tgaagtgatt acgccttccc tgacctgggt ttcaaccctc aatatgattt ccttgttggg
      361 tgcaacgccg gtaatggtgg atgtcgaccg cgatacgctg atggtcacgc ctgaagctat
      421 cgagtcagcc attacgccac gcactaaagc catcattccg gtgcattatg ccggtgcgcc
      481 agcagatatt gacgccattc gcgccattgg cgaacgttac ggcatcgcag ttatcgaaga
      541 tgctgcccat gccgtcggta cgtattacaa agggcgacat attggcgcaa aaggtaccac
      601 tattttttca tttcatgcca ttaaaaatat tacctgtgct gaaggtggcc tgattgtaac
      661 tgataatgaa aaccttgccc gccagctacg gatgctgaaa tttcacggtc tgggtgtcga
      721 tgcctatgac agacaaacct ggggccgtgc accgcaggct gaagtcttaa caccgggcta
      781 taagtacaat ctgaccgata ttaacgccgc gattgccctg acacagttag tcaaattaga
      841 gcacctcaac acccgtcggc gcgaaattgc ccagcaatat cagcaagcac tggcagctct
      901 cccctttcag ccattaagcc ttcccgcctg gccgcacgtt cacgcctggc atctgtttat
      961 tattcgtgtc gatgaacaat gttgtggtat cagtcgaaat gcgttgatgg aagcgttaaa
     1021 agaaagaggc attggtaccg ggttacattt ccgcgccgct cacacacaaa aatattatcg
     1081 cgagcgtttt cccacgctgt cgttaccgaa taccgaatgg aatagcgaac gcatctgttc
     1141 gttgccgctg ttcccggata tgactaccgc cgatgccgac cgcgtcatca cagcccttca
     1201 gcaactcgca ggacaataag ccatgtttga aatccaccct gttaagaaag tctcggtggt
     1261 tattcccgtt tataacgagc aggaaagctt accggaatta atcaggcgca ccaccacagc
     1321 ctgtgaatcg ttggggaaag agtatgagat cctgctgatt gatgacggca gtagcgataa
     1381 ttccgcgcat atgctggtcg aagcctcaca agcggagaac agccatattg tgtctatttt
     1441 gcttaaccgc aattacgggc aacattcagc gattatggcg ggattcagtc acgttactgg
     1501 cgacttaatt attacccttg atgccgatct ccagaatccg ccagaagaaa tcccccgcct
     1561 ggtggcaaaa gccgatgaag gttacgacgt ggttgggact gtacgccaga accgccagga
     1621 cagctggttt cgtaaaaccg cttcgaagat gattaaccgg cttattcagc gcaccactgg
     1681 caaagcgatg ggtgactacg gttgtatgct gcgcgcctat cgccgtcata ttgtcgatgc
     1741 gatgttgcac tgccatgaac gcagcacctt tatcccgatt ctggcgaata tcttcgcccg
     1801 ccgtgcaatt gaaattccag tacatcatgc cgagcgtgag tttggtgaat ccaaatacag
     1861 ttttatgcgc ctgattaatt tgatgtacga cctggtgacc tgccttacca caacgccgct
     1921 acgtatgctg agtctgctcg gtagcattat tgcgattgga ggttttagca ttgcggtgct
     1981 gctggtgatt ttacgcctga ccttcggacc acaatgggcg gcagaaggcg tctttatgct
     2041 atttgccgtg ctgtttactt ttattggcgc tcagtttatc ggcatgggat tactcggtga
     2101 atatatcggc aggatctaca ccgatgtccg cgcccgcccc cgctattttg ttcagcaagt
     2161 tatccgtcca tccagcaagg aaaatgaata atgaaaaccg tcgtttttgc ctaccacgat
     2221 atgggatgcc tcggtattga agctctgctg gctgccggtt acgaaattag cgccattttt
     2281 acccataccg ataatcccgg tgaaaaagcc ttttatggtt cggtggctca tctggcggcg
     2341 gaaagaggca ttccggttta tgcgccggat aacgttaatc atccgctgtg ggtggaacgc
     2401 attgcccaac tgtcaccaga ggtgattttc tctttttatt atcgccatct tatttgcgac
     2461 gagattttgc agctcgctcc cgcaggtgca tttaatctgc acggttcgct gttaccaaaa
     2521 tatcgtggtc gcgcgccgct gaactgggtg ctggtgaacg gtgaaacgga aacgggcgtt
     2581 acattgcacc gaatggtaaa acgtgccgat gctggggcca ttgtggcgca actgcgcgtt
     2641 gccattgcgc cagacgatat cgctattacg ctgcatcata aattgtgcca tgccgcgcgc
     2701 cagctactgg aacagacatt acccgccatt aaacacggta atattctgga aatcgcccag
     2761 cgcgaaaacg aagccacctg ttttggtcgc agaacgccgg atgacagttt ccttgaatgg
     2821 cataaaccgg catccgtact gcacaacatg gtacgtgccg ttgccgatcc gtggccgggt
     2881 gccttcagct atgttggcaa tcagaaattc accgtctggt cgtcgcgtgt tcatcctcat
     2941 gccagcaaag cacagccggg gagcgtgatt tctattgcac cgctgttgat tgcctgtggc
     3001 gatggcgcgc tggaaatcgt caccggacag gcgggcgacg gcattactat gcagggctcg
     3061 caattagcgc agacgctggg cctggtgcaa ggttcacgct tgaatagcca gcctgcctgc
     3121 accgcccgac gccgtacccg ggtactcatc ctcggggtga atggctttat tggcaaccat
     3181 ctgacagaac gcctgctgcg cgaagatcat tatgaagttt acggtctgga tattggcagc
     3241 gatgcgataa gccgttttct gaatcatccg cattttcact ttgttgaagg cgatatcagt
     3301 attcattccg aatggattga gtatcatgtc aaaaaatgtg atgtcgtctt gccgctggtg
     3361 gcgatagcca cgccgattga atatacccgc aacccgctgc gcgtatttga actcgatttt
     3421 gaagagaatc tgcgcattat ccgctactgc gtgaagtacc gtaagcgaat catcttcccg
     3481 tcaacttcag aagtttatgg gatgtgtagc gataaatact tcgatgagga ccattctaat
     3541 ttaatcgtcg gcccggtgaa taaaccacgc tggatttatt cggtatcaaa acaattactt
     3601 gatcgggtga tctgggccta tggcgaaaaa gagggtttac agttcaccct cttccgcccg
     3661 tttaactgga tgggaccacg actggataac cttaatgcgg cacgaatcgg cagctcccgc
     3721 gctattacgc aactcattct caatctggta gaaggttcac cgattaagct gattgatggt
     3781 ggaaaacaaa aacgctgctt tactgatatt cgcgatggta tcgaggcgtt ataccgcatt
     3841 atcgaaaatg cgggaaatcg ctgcgacggt gaaattatca acattggcaa tcctgagaac
     3901 gaagcgagca ttgaggaact gggcgagatg ctgctggcga gcttcgaaaa acatccgctg
     3961 cgccatcatt tcccaccgtt tgcgggcttt cgcgttgtcg aaagtagcag ctactacggc
     4021 aaaggatatc aggacgtaga gcatcgtaaa ccgagcatcc gcaatgccca ccgctgcctg
     4081 gactgggagc cgaaaattga tatgcaggaa accatcgacg aaacgctgga tttcttcctg
     4141 cgcaccgttg atcttacgga taaaccatca tgaccaaagt aggcttacgc attgatgtcg
     4201 atacctttcg tggcacccgt gaaggcgtgc cgcgtctgct ggaaatcttg agtaagcata
     4261 atattcaggc cagcattttt ttcagcgtcg gcccggacaa tatgggccgc catctctggc
     4321 gactggtgaa gccacagttt ttgtggaaga tgctgcgctc aaacgcggca tcgctttatg
     4381 gctgggatat tttactggca ggtacggcct ggccaggtaa agagattggt catgccaatg
     4441 ccgatatcat tcgtgaagcg gctaaacatc acgaagtcgg cctgcacgcc tgggatcacc
     4501 atgcctggca agcccgtagc ggtaactggg atcggcaaac aatgatcgac gatattgcac
     4561 gcggtcttcg cactctggaa gagattatcg gtcaaccggt aacctgttct gccgctgcgg
     4621 gctggcgtgc cgaccagaag gtgatcgaag caaaagaagc gttccatttg cgctacaaca
     4681 gcgattgtcg tggggccatg ccgttccgtc cattgctcga atcaggaaac cctggcactg
     4741 cgcaaattcc ggtgacctta cccacctggg atgaagtgat tggtcgggat gtgaaagcag
     4801 aagattttaa cggttggtta ctcaaccgca tcctgcgaga taaaggcacg ccggtttata
     4861 ccattcatgc agaagttgaa ggctgcgctt atcagcataa ttttgtggat ctcctcaaac
     4921 gcgcagctca ggaaggcgtg acattttgcc ctttaagcga actgttatca gagacgttgc
     4981 cgctcggaca agttgttcgc ggaaatattg ccggacgtga aggctggctg ggttgccaac
     5041 aaattgcggg tagtcgctga tgaaatcggt acgttacctt atcggcctct tcgcgtttat
     5101 tgcctgctat tacctgttac cgatcagcac gcgtctgctc tggcaacccg atgaaacgcg
     5161 ttatgcggaa atcagtcgag aaatgctggc atccggcgac tggattgtgc cccatctgtt
     5221 agggctacgt tatttcgaaa aacccattgc cggatactgg attaacagca ttgggcaatg
     5281 gctatttggc gcgaataact ttggtgtgcg ggcaggcgtt atctttgcga ccctgttaac
     5341 tgccgcgctg gtgacctggt ttactctgcg cttatggcgc gataaacgtc tggctctact
     5401 cgccacagta atttatctct cattgtttat tgtctatgcc atcggcactt atgccgtgct
     5461 cgatccgttt attgcattct ggctggtggc gggaatgtgc agcttctggc tggcaatgca
     5521 ggcacagacg tggaaaggca aaagcgcagg atttttactg ctgggaatca cctgcggcat
     5581 gggggtgatg accaaaggtt ttctcgccct tgccgtgccg gtattaagcg tgctgccatg
     5641 ggtagcgacg caaaaacgct ggaaagatct ctttatttac ggctggctgg cggttatcag
     5701 ttgcgtactg acggttctcc cttggggact ggcgatagcg cagcgggagc ctaacttctg
     5761 gcactatttt ttctgggttg agcatattca acgctttgca ctggatgatg cccaacatag
     5821 agctccgttc tggtactacg tgccggtcat cattgccggt agcctgccgt ggctgggatt
     5881 actccccggt gcactgtaca caggctggaa aaaccgcaag cattccgcaa ccgtctattt
     5941 gttgagctgg acgataatgc cgctgctgtt tttctccgtc gctaaaggta aattgcccac
     6001 ctatattctt tcctgctttg catctctggc aatgctgatg gcgcattacg ctttgctggc
     6061 agcaaaaaat aatcctctgg cgctgcggat taatggctgg attaacatcg cttttggcgt
     6121 cactggcatt attgccacat ttgtggtctc cccgtgggga ccaatgaaca ctccggtgtg
     6181 gcaaaccttc gagagctata aagtcttttg tgcctggtcg attttttcgc tatgggcatt
     6241 tttcggctgg tacaccttaa caaacgtcga aaagacctgg ccttttgccg cgctttgccc
     6301 gctggggctg gcgttgctgg taggattttc aattcctgac agagttatgg aaggaaaaca
     6361 tccgcaattt tttgtcgaga tgacacaaga atcacttcag ccaagccgct atattcttac
     6421 tgatagcgtc ggtgttgccg caggtctggc atggagcctg caacgcgatg acatcatcat
     6481 gtatcgccag acaggtgagt tgaaatacgg ccttaattat ccggatgcga aagggagatt
     6541 tgtcagcggt gatgagttcg caaactggct taatcaacat cgtcaggagg ggattattac
     6601 tctcgtgctt tcggttgacc gcgatgaaga tatcaacagt ctcgccattc cgcccgcaga
     6661 tgccatcgat cgtcaggagc gtctggtgct gattcagtat cgtcccaaat gatctggcta
     6721 acattagtct ttgccagctt gcttagcgtt gccgggcagt tgtgtcagaa acaggcaacc
     6781 tgctttgtgg cgataaacaa acggcgcaaa catatcgtgc tgtggctggg actggcgctg
     6841 gcttgtcttg gtcttgccat ggtgctctgg ctgctggtct tgcagaacgt accggtaggc
     6901 attgcctacc cgatgttaag tctgaatttt gtctgggtga cgctggctgc agtaaaactg
     6961 tggcacgaac cggtatcgcc gcgtcactgg tgtggggtgg cgttcattat tggtggcatt
     7021 gtgatcctcg ggagtacggt gtaatgggcc tgatgtgggg attattcagc gtcattattg
     7081 cctcagttgc gcaattaagc ctgggttttg cggcgagtca tctgccgccg atgacgcact
     7141 tatgggattt tatcgcggct ctgttagctt ttggcctgga tgcccggata ctgttactcg
     7201 ggctgctggg ttatctgctt tccgtttttt gttggtataa aacgctacat aaacttgcgc
     7261 taagcaaagc ctatgccttg ctcagtatga gttatgtgct ggtatggatt gcttcaatgg
     7321 tacttcccgg ctgggaaggg actttttcgc tgaaagcact actgggagta gcctgtatta
     7381 tgagcgggtt gatgctgatt ttcctgccca tgacaaaaca acgttactga gttttccctg
     7441 ccactttaca ctgccgttcc cactcatcgg ggctataact gcttgccgag aggactttca
     7501 ccgtatgcag cttttcacga ttaatacaat aaagcgcatt ctgcaaaggc gagagtaaat
     7561 cccccacttt cacggcgaag tcactcttca cttcggcgat catttttatc gcgccgccag
     7621 catcgcagag cataagtaca tgtctgttat cttgtttatt gcagcacgat tttttgacca
     7681 gccattccat tgcattatcc tgtttgctaa gagttttcac atcaatttac agattaacag
     7741 ataaactctt tatcaacggc aatggaatgg cagaaatgag aagtgaaacg gagggttaat
     7801 tattgctgac gttgcaccca ctcttttagc gcctgacgtg aaattttaat accgccgttt
     7861 ttcagttcag gcggcaacgt taaccaacgc accggttgct gaaaacgggc cagcttatct
     7921 ttcacccatt cactaagatc aacgctctcg tggtcatact ccatcaccgc caccggtcga
     7981 tgaccaaact ccttgtcggc aacggggacg ataaacacct gcaaaaccgc aggatgtgca
     8041 gcaattacgc gctcgacttc ttccggctga ataccctctc cgccactgaa gaatagattg
     8101 tctaaacgtc cgacaatggt cagcttgcca ttatgcatct caccgcgatc gcgcgtagcg
     8161 taccagcctt cgtcattaac cagtgaaacc agttgcccgt tacgccagta accttctgcc
     8221 atactggcag cccgcagcca cacttcatta ttaacgattt tcacttcccg acccggcagc
     8281 ggcgaaccaa cgtctgccag gccgtcggct tctttcgcac acaccgtgga ggcaaactcg
     8341 gtcagaccat agccgcaaaa gcaacgaatc ccctgctcgc gcgcctgttc cgtcaactcg
     8401 accgggatag ccgcgccgcc aagtaacacc gctttcaggg aaacggaact gcggttaacc
     8461 agcaaacgcc agagttgtgt tggcaccagt gaagcgtgag tacagcctgc cagcatttgc
     8521 tccaatggct gtttatcacg caccgtcatc cgcgcaccag cgtataacca gcgccacata
     8581 attccctgac cggagacgtg aaacagcggt aaagagagca accagtcatc gtgatcgcca
     8641 aacggaatca acgataacac cccttcggca ctggcaagat gggcctgata agtatggaca
     8701 gcggctttcg gcaacccggt agaacccgag gtcaacgtca ttgagcacag acgcgtcggc
     8761 tgccacgtag cggcatgtgc gccttcaacc cgctgaatgt gcagcgacgt taacgccgga
     8821 aacgtgtttt ccccatccgg caccagcgca aattgcagcg tcagattggg cagcaattct
     8881 tcaagcaacg gttgcggcag ctgagggttc acgggcaaca cccgcgcccc gcattgcagt
     8941 aacgccagcc aggcgagcag cgtttgcggc gtattccacg cccgcaacat cacgccgctg
     9001 ccctcaacaa cgccctgcgc cgcaaagcct gaggctaatt catcgacgcg agcacaaagc
     9061 tcgcgccagt taagttgctc gtcattaaga cgtaaggcga tggcttctcc ccgcacttgc
     9121 cgccagtgac gccacggcca gtcagagaag atcataacaa ccgctccagt gcatcaactt
     9181 ccacgacagg cagcgtgcta cccggccagc gacgtacctg ctgcgcctgc atcagatcca
     9241 gcgtgtccag ccctggaatg gtgtccggcg ttaaccaggc ggcaatccgc gccagttgcg
     9301 ttaagcctaa gctcgattca atggaagaac tgatcaccgc cgtcagcccc agcgcgtgcg
     9361 ccgcctgtac ctgctcgcgt actttctcca gactgcccgt gagcgtgggt ttgataacta
     9421 ccgcgcgcac gccctcttca gccacaaagg caaaatccgg ctcgcgcaag ctttcatccc
     9481 aggcaatggc aatgccggtt tcacgggcaa acgctcgcga atcatcgcgg gttttgcacg
     9541 gctcttcgag aaacgcgatg cggtggcgat aatccgggtt aacatatttg gcaaactgct
     9601 ggcctttcag cggtgtccag gcgcgatttg cgtcaagacg caaatgcaga tccggaattg
     9661 cctccagcaa cagattaacc accatgccgt cgcgcacggc ttcgtacaat cccactttga
     9721 ccttcgccac tttctcgcct ggcatatcag caagtttgag gatcagatcg tcaggatcgc
     9781 cattacacag tggtgccgca cggtaattgg ccgcctgcgg gagcgtatct gccagctccg
     9841 ccaatgcaca gcttacgcca aaggcaacgg aaggcatctg cggtagctcg caatcgcctg
     9901 ccagccagtt atttacccag gcaagcagca cgctttgcgc ctcttcccag gtttcctggc
     9961 tgaagcccgg cagtggggag atctcccccc acccttcgcg ctcgccctca cgcaggcaaa
    10021 cgtacagccc gtcgcgggtt tttaaccgcc tgtcgcgcag aaccaccccc gcgtccatgg
    10081 ggatctgcca gcggtatacc tgcgcgctac gcattacgga ttccgtttaa atttgctgaa
    10141 atcaggctga cgtttctggt tgaaagcgtt gcgaccttcc tgaccttctt ccgtcatgta
    10201 gaacagcatg gtggcgttgc ccgccagctc ctgcagcccc gcctgcccgt cacagtcggc
    10261 gttcagcgca gctttcaggc agcgcagcgc catcgggctg ttttgcagca tttcgcggca
    10321 ccaacggacg gtttcttttt ccagatccgc cagcggtacc acggtgttga caaggcccat
    10381 atccagcgcc tgttttgcgt cgtactgacg gcacaggaac cagatttctc gcgctttttt
    10441 ctgcccgacg atacgagcca tgtaggaagc gccccagccg ccgtcgaagg aaccgacttt
    10501 cgggccagtc tgaccgaaga tggcattatc tgctgcgata gtcaggtcgc acatcatgtg
    10561 cagaacgtga ccaccgccga tggagtagcc agccaccatc gcgacaaccg gtttcggaca
    10621 ggtacggatc tgacgctgga agtccagcac gttcaagtga tgtacgccgg aatcatcttt
    10681 atagccgccg taatcaccac gcactttctg gtcaccaccg gagcagaacg ctttatcgcc
    10741 tgcgccagtc agaatgatca cgccgatatt gtcgtcataa cgcgcatctg ccagtgcctg
    10801 gatcatctct tttaccgtca gaggacggaa ggcattgcgc acctgcggac gattaatggt
    10861 gatttttgcg ataccgtcgg tggatttttc ataacgaatg tcctcgaaac cttcggagca
    10921 gtcgtgccat tcaaccggtg cgtaaagcat tgcttcatca ggataaatca tattgtgtcc
    10981 tttagtcaga aacgcaagat ctgcgccaga cttgcgatta cgccagcggg attttcccga
    11041 tgcgcgttat gtccggcgcg aggaatgaca tggcagtcgg cagccagttc cgccgccagg
    11101 gcgcggaatt tgctgtcacg ttcaccacat aaataataaa acgcaaatgt gcgggcgcta
    11161 aggttagcac gtaaatcagg ctggacggcg agagaagtcg cctccagcat ggcggcaagg
    11221 gttgcgccat tattgttgct gcgcagcgcc accagctccc ggcgttgatc gtcattgagc
    11281 gaggcaaaaa caggctgttg ataccagtcg gcaaataccg ccgttaacgg ttctgtgcaa
    11341 aaacgctgcg cccattggcg atcggaacgc tgacgttccg cacgttgttc agcattttgc
    11401 agccccggat gcccgccttc gacaataacc ccacaaagcc ccgccagccc ctggcaagcc
    11461 gccatcatcg ccacccgtcc gccaagcgag taccccacca gccagaagtc aaggatgttg
    11521 taactaacca aggttttacg cagtaagtcg gtgacatcat caaatccatc gacgctaatc
    11581 gccgccgaac caccatgacc tgggagatca acatacaacc gtgagtagtc ggcaaacgcc
    11641 tcgcccactt cttgccattc gtggcaatcg ccggaaaaac cgtgcaaaaa caccagccag
    11701 ggtaaacctg gttttccgtg ttttgcctgc gcgtgcagga tcataaatgg cttacctgcg
    11761 ccagaagttg ctggagcgtt tgcgcaccat cggtgtcgtt aaccaccatt tcaatcaccg
    11821 tggtggttgg cgtgcgccag gcgtcggcaa atgccgtttc aagttcctgc cagttttgcg
    11881 gacgatgata tttcagctcg aacatcgcgg cggcgtgctc aaaatggacg ttttgcggca
    11941 tcagatagaa acgctcacgc tcgctttgcg gcgttggcaa cagcgagaaa atttgcccgc
    12001 cgttgttgtt caccacaatt aataccagcg gcgcagaaac ctgacgcaat aacgccagcg
    12061 cgttgagatc gtaaagtgcg gagagatcgc ccacaatcgc cagcgtcggt ttgccgcttg
    12121 cccgctgaac gcctgcggcg gtcgaaagca gtccgtcgat accgctggcc cctcggttgc
    12181 tgtacaccgg gtaacctgcc ggaagttgcg aaagcgcatc aatcagacgt accaccaggc
    12241 tgttaccaac aaacaattgc ccctgttcag gcagatagtc gctgatgcga tgcgccagtt
    12301 gcgcttcgcc aaacgcatca cggcgggcaa taaccgcctg cattgcctgt tccgccaggc
    12361 gcgggatttc aacgcaccag ggctggcgtt tttctgccgg atgcagctcc agccagtcgg
    12421 caatattggc aattaagcga cgtccgcgat ggtgtgccgg atcaagtcgc ccttcaatgt
    12481 catcaacaat ccagtactct tcgggttcac agcttgcctg ccattgcagg agccgtttcc
    12541 ccgtcaggct gcttcccagt tgcaccacaa tttgcgcctg ctgcagctcg ctggtcgctt
    12601 tggcattgcc taaccaaaga tcggcacacg gcagcggctg cccggtttgt gacagcacat
    12661 cgccaatcag cggccagcca agagtttgcg cccacagggc aacttttttg ccctcttccg
    12721 cactcatgcg cccggcaacc accacgccgc gcttttgtcg ccagaagaac cagtcgcgct
    12781 gtttttcact ttccagacga ggcgcttcac gcagccacgg tttgtcgtcc tgccaccagt
    12841 cacccagacg ctgttgccag ctaagcccgg tatcgtccat ttcgccatac agcggttcag
    12901 caaacgggca gttgatatgg actccccccg catgaagcgt accgagagcg tggtcgatgg
    12961 tagaaaccag ccaacgtgcg gggatatcct gggtcgggcg cggcaatgaa atactgtgcg
    13021 tggggtgaga ggcgaacatt cccggctggc gaattgcctg attcgcgccg cagtcaatta
    13081 gctccggcgg gcgatcggcg gttaagagaa tcagtttttc tccggttaac ccggcttcaa
    13141 tcagtgccgg atagagattt gccaccgccg tgccggaggt cacaatcacc gccaccggct
    13201 gcttgctgac tttcgccagc cccaacgcca gatgccccag tccacgctca tcgaaatggg
    13261 tgtggtgaat gaatgcggaa ttctccgccg ccgctaacgt taacggtgta gaacgcgagc
    13321 caggggcgat acagatgtgt ctgacgccgt gacgcgttaa tgcttccaga atgaccgccg
    13381 cccagcgtcg gttaaatgcg cttactgaca tgagtttgtc cggtatcaat attgcggcta
    13441 agtataagga gctacaaaaa tagattattg atatgaatcg gtaatgatgc gactcattac
    13501 tattccattt gtaataaagt acgcagccct gccgctttgt tgtcgatttc ctgccactct
    13561 tgctcggggt cggaaccacg gacaatgccc gcgccagcat ataatcgcac gacattgccg
    13621 ctaatttttg ctgagcgcag ggaaacgcag aattcgcttt gttgtaatga gagatagccc
    13681 gcagaaccgg cgtaccattc tcgggtgaac ggttcgtgac gggcgataaa ctgtcgcgcc
    13741 agatcgcgcg gtaagccagc aactgctgcc gttggctgca actgatgtaa acagatcaca
    13801 tcatccgctt tgttgagtga agtccagata cagcggcgaa gatgctgcac tttacgcaga
    13861 cgcagtacct gcggcggtaa aacatccagc gtctgggtat cggcctgtaa tcgttggcag
    13921 atatcttcca ccaccagcat gttctcgcgc tggtttttat catccgccat cagccactct
    13981 cctaactgct gcgcctgctt atcatcagga tgatttgcta ctgttcccgc cagcgcttca
    14041 gtacgcagcg ctttgtcacg ccgccgccat aaccgttccg gtgaagagcc aagaaaagca
    14101 ttttcgccat caaaggccat gtaaaaatgg tagcaattca gattcagtcg acgactggca
    14161 gccatcatcg ccgccgcgtt gaccggactt gcgaaatgca ggtcagttgc ccgagcgagc
    14221 accactttgt cgagctcacc ttcggcgatg gttttcgttg ccagttcgat taattgcgtc
    14281 cagcccgttt tgtccggcca gtgttgttct cgcgtggtgg ttaagtgtaa cccaggcaag
    14341 ggcttgatac tcaccagtgt ggcgataaat tcttttgcct gaatcgcatc gtgctgaagg
    14401 gagctttcgc tgaatagcgt cagccgcagc gtggctttac cgccacagcg tcgccattcc
    14461 aggcggggta aaagtaaatt gccctgcgac gggtcaaaag cattcagccc ccaaatgcgt
    14521 aagtcggcgt gttccgggtg ctggcgaaga aaacgttgtg cctggtccaa cgacgtaaaa
    14581 cgggtaatcg cgcccaggac gacagcttct tcatcaccat tacgttgttg ccagtagaat
    14641 tgcgggtatg tttgctgact ggccagccag ctcaaggcat caaaagcgtc tttgagaggg
    14701 aaaggaatat cgataacccg aatgccgggt gtcgccggaa tctcttgcga caaatggcgc
    14761 agtagatttt ccagcgccgt agtaagtgat tgcacgcgga cctctccctg ttaaaagcct
    14821 cacattatac ggggtactac aaaaaaaatg cagtaccccg gtgtagggag gttttaacgg
    14881 cgtgccagca acagtcctag taccagccca acggccgcgc ccacaccaat tccttgccag
    14941 ggtttttcgt ggacgtagtc atcagcacga taaactgcct gcttcgcccg atagtaataa
    15001 ctgtctgaag cctggctaac ccgttttttc acatcatcca gcgctttttc tgcacgcgct
    15061 ttcagctcaa catatttctg atcggcggga tcgccagagg agcggagcac ctcttccagt
    15121 gtttcactaa gcagcgtcag gtcgtcatcg atacgtgtat caccaaactg attagacatt
    15181 ctcgttctcc atttgcgtaa aacctgtcac taactataga caagggttgt tgattacgcc
    15241 tgaattacct cgcgtgccat cccaatgtgt gggataccat cttcttcata gacctccgtc
    15301 acagggataa aaccgaaact ctggtagaag ttttgcagat gcgcctgcgc cccgaggtaa
    15361 acaggcttat caggccagtg atgcgtacat gtttcgagtg ttttactcat cagctgctgc
    15421 cccacttttt cgccacgcaa cgcttcactg acaatcaccc gacctataac gaccggctca
    15481 agatcatcat cacttttcag aatcctcgca tatgccacca gttcatcatt tttccaaccg
    15541 agaatatggc gattatcccc cgtcaaatca tcaccatcga tatcctgata agggcaattc
    15601 tgctcgacaa caaacaccgc gcaacgcagt tgtaataagg cataaagctg ggaaaccgat
    15661 aattcagagt gatgcagatc ttgccattca atcataatgc tctcctttta gatgtatgcc
    15721 ttatactagc cggaatattt tttgaaacgt gatgaactca tggaattaat ttttttaggt
    15781 acttcggccg gtgtgccaac ccgcacgcgc aatgtcacgg caatattgct taacctgcaa
    15841 catccgaccc agagcggtct ttggttgttt gactgcggtg aaggcaccca gcatcagcta
    15901 ctgcataccg cctttaaccc tggaaaactg gacaagattt ttatcagtca ccttcatggc
    15961 gatcatcttt ttggtttacc cggcttgctg tgcagtcgtt ctatgtcagg cattatccaa
    16021 cccttaacga tttatggtcc gcaaggtatc cgtgaatttg tggaaaccgc gctgcggatt
    16081 agcggctcat ggaccgatta tccgctggaa attgtcgaaa ttggcgctgg cgaaattctc
    16141 gatgatggcc tgcgcaaagt aaccgcttat ccgctggaac acccactgga atgttatggc
    16201 tatcgtattg aagaacatga taaaccgggt gcattaaatg cccaggcatt aaaagctgct
    16261 ggcgtgccgc ctggcccact gtttcaggaa ttaaaagcgg gcaaaacaat cacgctggaa
    16321 gatggaaggc agattaacgg cgcagattac ttagctgctc cagtgccagg taaagcgctc
    16381 gctattttcg gcgataccgg cccctgcgat gccgcacttg acctagctaa aggtgtcgat
    16441 gtcatggtgc acgaagcgac gctggatata accatggaag ccaaagccaa tagtcgcggc
    16501 catagctcta cacgccaggc tgcgacacta gcccgtgagg ctggagtcgg caagctaatc
    16561 attacccacg tcagctcgcg ctatgatgac aaaggttgtc aacacctgtt acgtgaatgc
    16621 aggtcaattt tcccggcgac tgaactggcg aatgatttca ccgtgtttaa cgtttaactc
    16681 tgcttattat taaaaacagg gtgaaacttg ccctgttata gcaacccgcg ctacatatat
    16741 ccacccacac aggcatattg agcgattttt gatatttatt catctcctta cgcactatat
    16801 tcagagcaat aagttgcctc ttttcatctt gattcagcac aacctttgca actaaaagga
    16861 gcccatgatg gttacagttg tcagcaatta ttgtcaatta tctcaaaccc aactcagtca
    16921 gacatttgca gaaaaattta ctgtgaccga ggaattactg cagtctttaa aaaaaacagc
    16981 gttatccgga gatgaagaaa gtattgagtt actgcataat attgcgttag gttatgatga
    17041 atttggaaaa aaagctgaag atattcttta ccatattgtt agaaacccaa caaatgagac
    17101 cctatcgatt atccgactta ttaaaaatgc ctgtttaaat tatataattt ggcacatacc
    17161 gcaaccaact cccaccttaa accaactggt ccggataatt cagatgttct cctgttcaaa
    17221 aagctattct ccccttcgaa attaatgaca attatcggtg atgaaattcc tcttatatct
    17281 gaaaaacagt cgctttcaaa ggtgctttta aatgatgaga ataatgaact gagtgatggt
    17341 acaaacttct gggataaaaa tcgtcaatta accacagatg aaatagcttg ctatcttcag
    17401 aagatcgccg ctaatgcaaa aaatactcaa gtcaattatc ctactggtct ctacgtcccc
    17461 tactccacca gaactcacct ggaagacgct ctcaatgaaa atattaagag cgatccatca
    17521 tggccgaatg aagtccagtt attccccata aatactggcg gacactggat attagtttcg
    17581 ctacagaaaa tagtaaataa aaaaaataat aaactgcaaa taaaatgcgt catattcaac
    17641 tcattgcgtg cactaggcca tgata
//
LOCUS       DAJOSY010000089        17472 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJOSY010000089 DAJOSY010000000
VERSION     DAJOSY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.089"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            95..613
                     /locus_tag="OHN27_003223"
     CDS             95..613
                     /locus_tag="OHN27_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCQ2287610.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            603..1829
                     /gene="dgcN"
                     /locus_tag="OHN27_003224"
     CDS             603..1829
                     /gene="dgcN"
                     /locus_tag="OHN27_003224"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCQ2287611.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1845..2327
                     /locus_tag="OHN27_003225"
     CDS             1845..2327
                     /locus_tag="OHN27_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCQ2287612.1"
                     /translation="MIKHLVAPLVFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWPLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2403..2750)
                     /gene="rplS"
                     /locus_tag="OHN27_003226"
     CDS             complement(2403..2750)
                     /gene="rplS"
                     /locus_tag="OHN27_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCQ2287613.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2792..3559)
                     /gene="trmD"
                     /locus_tag="OHN27_003227"
     CDS             complement(2792..3559)
                     /gene="trmD"
                     /locus_tag="OHN27_003227"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCQ2287614.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3590..4138)
                     /gene="rimM"
                     /locus_tag="OHN27_003228"
     CDS             complement(3590..4138)
                     /gene="rimM"
                     /locus_tag="OHN27_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCQ2287615.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4157..4405)
                     /gene="rpsP"
                     /locus_tag="OHN27_003229"
     CDS             complement(4157..4405)
                     /gene="rpsP"
                     /locus_tag="OHN27_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCQ2287616.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4542..5903)
                     /gene="ffh"
                     /locus_tag="OHN27_003230"
     CDS             complement(4542..5903)
                     /gene="ffh"
                     /locus_tag="OHN27_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCQ2287617.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6070..6861
                     /locus_tag="OHN27_003231"
     CDS             6070..6861
                     /locus_tag="OHN27_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCQ2287618.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMGNIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6882..8168
                     /locus_tag="OHN27_003232"
     CDS             6882..8168
                     /locus_tag="OHN27_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCQ2287619.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLLILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8223..8816)
                     /gene="grpE"
                     /locus_tag="OHN27_003233"
     CDS             complement(8223..8816)
                     /gene="grpE"
                     /locus_tag="OHN27_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCQ2287620.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8939..9817
                     /gene="nadK"
                     /locus_tag="OHN27_003234"
     CDS             8939..9817
                     /gene="nadK"
                     /locus_tag="OHN27_003234"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCQ2287621.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9903..11564
                     /gene="recN"
                     /locus_tag="OHN27_003235"
     CDS             9903..11564
                     /gene="recN"
                     /locus_tag="OHN27_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCQ2287622.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11713..12054
                     /gene="bamE"
                     /locus_tag="OHN27_003236"
     CDS             11713..12054
                     /gene="bamE"
                     /locus_tag="OHN27_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCQ2287623.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12116..12406)
                     /locus_tag="OHN27_003237"
     CDS             complement(12116..12406)
                     /locus_tag="OHN27_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCQ2287624.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQQAEKSANK"
     gene            complement(12396..12833)
                     /gene="ratA"
                     /locus_tag="OHN27_003238"
     CDS             complement(12396..12833)
                     /gene="ratA"
                     /locus_tag="OHN27_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCQ2287625.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            13004..13486
                     /gene="smpB"
                     /locus_tag="OHN27_003239"
     CDS             13004..13486
                     /gene="smpB"
                     /locus_tag="OHN27_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCQ2287626.1"
                     /translation="MTKKKAHKLGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13701..14063
                     /gene="ssrA"
                     /locus_tag="OHN27_003240"
     tmRNA           13701..14063
                     /gene="ssrA"
                     /locus_tag="OHN27_003240"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14231..15451
                     /locus_tag="OHN27_003241"
     CDS             14231..15451
                     /locus_tag="OHN27_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2287627.1"
                     /translation="MPKVATKLTDTEIKKAKPTEKEFTLWDGDGLFLRIKPSGKKIWH
                     LAYAAPVSKKRSKMSLGFYPHLTLAQARALRDEYLSLLANGIDPQSHNEQKANALKDA
                     AEHTLQTVAKQWLEEKVKTSGISEDHAKDIWRSLEKNIFPGLGHLPIKEIRPKLLKQH
                     LDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGHRFSKPKKQNMPALPP
                     NELPRFMIALSNASIRLETRLLIEWQLLTWVRPGEAVRARWGDIDVKNKFWNIPPEFM
                     KMKRPHKVPLSKEALRILEKMQPISGHREWVFPSIKAPLNHMHEQTANAAIIRMGFGG
                     ELVAHGMRSIARTAAEESGKFRTEVLEAALAHSKKDEIIAAYNRAEYLVERAELMQWW
                     SDYVQAQKYKFMVA"
     gene            complement(15515..16291)
                     /locus_tag="OHN27_003242"
     CDS             complement(15515..16291)
                     /locus_tag="OHN27_003242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287628.1"
                     /translation="MKMSTLKLITVLIILVVWYLYFSHFNHWDNPLDLLNKEKWMGFS
                     DDKGDWGTFGDFVGGVLNPLLTFITIVMLINSLKLQREANEILIKDERRQEKDLMLKR
                     FEDSFYNLISSEREEFSSFSITINNTEFKSSNAVSEIEQAIWDFIQAQISDNGECEVI
                     EVRRFIDNIDNESSMSIFSMVRCVYVVFKVVKEKCPEEHRDYYLDIFVNMMPVKLVNL
                     VSIMSVYMDWKSISYINGNGKGFIDNEGVQKYINTLKDLH"
     gene            complement(16292..17119)
                     /locus_tag="OHN27_003243"
     CDS             complement(16292..17119)
                     /locus_tag="OHN27_003243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287629.1"
                     /translation="MINLDMDVVVGHGQDDFEELEMTSGIKVFSGASDITQITASTIL
                     DDSVPKKSTSIHGLRNIFKHTFDGSFGQKFCVRIEDPEKITRFNEIGKTVFFDVMSYY
                     ISKGMGEVHNLTNQASIDLVNELEPLERTLLNRIQDPIENMHRTVDKQGYNVLLRRRT
                     PQRKIIVAKMDSATLYNINIEHESPQPVQENVIITRFNMLTGTGRLLLDRQSDSIAFR
                     HALNWEHVLQSQKNKFSRNLDVNNRGGRDAFIPITISAIELRNHIGELKTYIIQEVL"
BASE COUNT         4610 a         4458 c         3935 g         4469 t
ORIGIN      
        1 cacattgatg acggtcgcat gaaaaaatga tattttaatg agcattctga aaaaatatcg
       61 atgcatttcg agcgaagatg gtggggatcc ctgaatgcgt ttttctcacc gactgttctt
      121 acttctgatt ctcctgctga cgggagcacc aatactcgct caggagcctt ctgacgttgc
      181 gaaaaacgtg cgtatgatgg tttccggtat tgtcagttac acccgctggc ctgcgctatc
      241 agggccacca aagttatgta tcttttcatc ttcacgtttt agtaccgccc tacaagagaa
      301 tgccgcgaca tctttgcctt atctgcctgt catcattcac actcaacaag aggcgatgat
      361 ttcaggctgc aatggttttt attttggcaa cgagtcaccg acattccaga tggaattaac
      421 cgaacaatat ccgtcaaagg cgttattatt aattgccgaa caaaataccg agtgcattat
      481 tggtagcgcc ttttgtctga tcatccacaa taatgacgtc agatttgccg taaacctgga
      541 tgccttatcg cgtagcggtg taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa
      601 aaatgatgga taacgataat tctcttaata agcgccccac gtttaaaaga gcattacgca
      661 acatcagtat gaccagcata tttatcacta tgatgctgat ctggttgctg ctttccgtga
      721 cctcggtgct gaccctgaaa cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa
      781 tgacttacag tctggaagca gctgtcgttt ttgccgatgg ccctgcagca actgaaacac
      841 tggcagcgct gggccagcaa gggcaatttt caactgcaga agtacgtgat aagcagcaaa
      901 atattctggc atcctggcat tacacccgta aggatccagg cgatactttc agtaatttca
      961 taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat ggtgaaacca
     1021 ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc tggttttcgc
     1081 tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc ctcacccgcc
     1141 atttgcacaa tggcctggta gaagcgctga aaaatatcac cgatgtcgta catgatgtgc
     1201 gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag tttcaccgct
     1261 tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt cgtttacagg
     1321 ctaaaaatgc gcagcttcta cgtaccgcgc tacatgaccc attaaccggg ctggctaacc
     1381 gcgcagcgtt tcgtagcggc atcaacacgt tgatgaacaa ttccgatgcc cgaaaaacgt
     1441 cggcgttact atttcttgat ggcgataatt tcaaatacat caatgatacc tggggtcatg
     1501 cgacgggcga tagagtcttg attgaaatcg caaaacggtt agctgaattt ggcgggctgc
     1561 gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat gatgtacagt
     1621 cggaatctga agtgcagcag atatgttcag cactgacaca aatctttaat ctcccgtttg
     1681 atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg atgaccattg
     1741 agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat caggccaaac
     1801 accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata aagcacctgg
     1861 tagcacccct ggttttcacc tcactaatac tgactggctg ccagtcccct cagggaaagt
     1921 ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc gccggcgact
     1981 ggccgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa ttgctcccgg
     2041 aaagccagca acagatccaa accatggcag ctaaattggc ctcgacaggg ctaacacatg
     2101 cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa ggcttatcat
     2161 tgaaacgggc gaatgtcgtg gccgatgcat gggctatggg tggacaaatt ccacgcagca
     2221 atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag accgcccagg
     2281 gccgcgccga gaaccgccgc gtcgcagtgg tgattactac cccttaaaac accagcaaac
     2341 agataaaaaa gggccagcca attggccagc ccttcttaac aggatgtcgc ttaagcgaaa
     2401 tcttagttaa gacgctcttt gatacgagca gccttaccag tacgctcacg caggtagtac
     2461 agtttagctt tacgaacagc accacgacgt ttgacagaaa tgctgtcaac taccggagag
     2521 tgagtctgga agacacgctc aacgccttcg ccgttggaaa ttttacgaac agtgaatgca
     2581 gagtgcagac cgcggttacg aatagcgata accacgccct cgaatgcctg cagacgtttt
     2641 ttggaacctt caacaaccca tactttcact tccacggtat cacccggacg gaaggaaggt
     2701 acgtcctgct tcatctgctc ttgttcaagt tgcttaataa tgttgctcat aatttaatct
     2761 cttatcctgg gtaaactgat atctcggggg cttacgccat cccatcatgt ttatgttgct
     2821 gttgtgcgtg ttccgttttg aactccgcca gcaaccttgc ttgctcttca gtcagagcca
     2881 ggttttccag aagttcaggt cttctaagcc aggtacggcc cagcgactgt ttcaaacgcc
     2941 agcgacgtat ctcggcatgg ttgcccgaca gtaacactgg cggaacttcc atcccttcta
     3001 acacctcagg ccgcgtatag tgcgggcaat ccagcaatcc ttcagcaaag gaatcttccg
     3061 ttgccgaggc ttcatgtccc agtactcccg gaataaaccg ggaaacggag tcaatcagcg
     3121 tcattgctgg taactcacca ccactgagaa cgtaatcgcc gattgaccat tcttcgtcaa
     3181 tttcggtttg gatcacgcgc tcatctatac cttcgtagcg accgcacacc agaatcaatt
     3241 tttgattcgt tgccagttcg ctgacgcccg cttgatcaag cttgcgtccc tgtggtgaca
     3301 gataaatcac ctttgcgcct tcacccgccg cggcttttgc tgcatgaatg gcgtcccgca
     3361 agggttgcac catcattaac atccccggtc cgccgccgta aggacgatcg tccacggtac
     3421 ggtgccggtc atgcgtgaag tcgcgaggac tccagctctg gatgctcagc aggccatttt
     3481 taactgcccg gccagttacc ccgtaatcgg taattgcgcg gaacatttca ggaaacaggc
     3541 taattatgcc aatccacata gcgccgtctt ttaccgttta tccggtggtt taaaaaccag
     3601 gatcccaatc tacttcgatt gaacgagtag tgagatcgac tttcttgata acctgcccat
     3661 cgaggaacgg tacgagacgt tccttgatac caaacgcatc tttcaggttt gccttaatga
     3721 cgagaacgtc attagatccg gtttccatca tatcgacgac tttaccgaga tcgtagcctt
     3781 cagtggttac tacctggcag cccatcaggt ctttccagta gtagtccccc tcttcaagct
     3841 gaggcagctg cgatgaatcc acgacaattt cacaattcgt cagcaggttc gccgcatcac
     3901 gatcgtcaac gcctttcagc ttgatgatca tgtcctgatt gtggtgcttc cagctttcca
     3961 gctggacttg ctgccactga cccgccttct ggataaacca gggctgatag tcaaaaatgc
     4021 tttcggcgtc ttcggtggaa gaaaacactc tgagccaccc acgaataccg taagacgaac
     4081 ccatttttcc caaaacgatg ggatcaacag gtgcttgcgc ggtgagttgt ttgctcatca
     4141 tgaccaccgt gacagattaa gctgctttgt ttacttcttt gatcagcgca gcaacgcgat
     4201 cagaaatagt tgcgccctgg ccaacccagt gagcgatgcg atccagatcc aggcgagtgc
     4261 cttcttcttt ttcgctagcg attgggttga agaaaccaac gcgctcgatg aagcgaccgt
     4321 tgcgtgcatt acggctgtca gcgacaacaa cctggtagaa cggacgcttt ttagcgccgt
     4381 gacgtgctaa acgaatagtt accataacat cctcttgtgt gaataaaaca accggacccc
     4441 atcgaggaac ggagtccggt gtcatattaa aagcccgaaa attttactga tttctggaga
     4501 aaatgcaatc agcagttaat aactctgtcg cataaggtca tttagcgacc aggaaatccc
     4561 ggcggcatca tccccttcat gcttctcatc atcttcgcca ttccgccctt cttcattttc
     4621 ttcatcatgc gctgcatgtc gtcgaactgc ttcagaaggc ggttaacgtc ctgcacctgc
     4681 ataccgcaac cggcagcgat acgacgctta cgcgaacctt tgatgatttc tggcttagcg
     4741 cgctctttca tcgtcatcga gttgatgatg gcttccatac gcaccagcac tttatcgtcc
     4801 atctgcgact taacattatc cgggatctgc cccatgcccg gcagcttgcc catcaggcta
     4861 gccatgccgc ccatattttt catctggcgc agttgctcaa gaaaatcgtt gagatcgaag
     4921 ccgtcgcctt ttttcagctt gctggctaat ttctctgcct gcgcgcggtc aactttgctt
     4981 tcgatatctt cgatcagcga cagtacgtcg cccatgccga gaatacgcga cgcgatgcgg
     5041 tccggatgga acggctccag cgcctcagtt ttctcgccaa caccgagaaa cttgatcggt
     5101 ttgccagtga tgtgacgaat agagagcgcc gcaccgccgc gggcatcgcc gtccactttg
     5161 gtcaacacta cgccggtaag cggtaacgct tcattgaatg cttttgccgt attggccgca
     5221 tcctgaccgg tcatggcgtc aaccacaaac agggtttcaa ccgggttaat cgacgcatgg
     5281 acttgtttga tctcgtccat catcgcttcg tcaacgtgca gacgaccagc ggtatccacc
     5341 agcagcacgt cgtagaattt cagtttggct tctttcagcg ccgcgttaac gatatctacc
     5401 ggcttctgac caacatcaga agggaagaaa tcaacgccca cctgctctgc cagcgtctca
     5461 agctgtttga ttgccgccgg gcgataaacg tcggcagaaa ccaccagcac tttcttcttg
     5521 tgcttctcgc gcaggaactt accgagttta ccaacgctgg ttgttttacc ggcaccttgc
     5581 aggcccgcca tcagcacgac cgcaggcggt tgcgccgcca ggttcagggt ctggttctct
     5641 tcgcccatcg ccgcaaccag ttcgttacgg acgattttga cgaactcctg ccccggcgtc
     5701 aggctcttat taacttcatg accaaccgct ttctctttta cgcgattgat aaactcacgc
     5761 actaccggca gagcgacgtc cgcctccagc agcgccatgc gcacttcgcg cagcgtatct
     5821 tttacgttgt cttcagtgag gcgtccacgg ccactgatat tgcgcagcgt gcgcgacaaa
     5881 cgatcggtta aattatcaaa cattgtctct cgcctggggt ggaaacggtt ggccgcaatc
     5941 gcgacacatc atcagtattt tgccgcagta taacatgaag gcgtctttgt tgttatgcaa
     6001 cggttggagc agcgttcacc tgacgctata ctgcttctct ttcttattgc tcaaactgtc
     6061 gacatcacta tgcccgtttt tgctctgctc gcgcttgtcg cctactccgt cagtcttgcg
     6121 ctgattgttc ccggtctgct gcaaaaaaac ggcggctggc ggcgcatggc tattatttct
     6181 gcggtcattg cgctggtctg ccacgcaatc gctctggaag cccgcatcct gcccgacggt
     6241 gatagcggac aaaacctcag cctgctgaac gttggttcat tggtcagttt gatgatctgt
     6301 acggtaatga ccattgtggc ttctcgcaat cgtggctggt tgctgctacc cattgtttat
     6361 gcctttgcgc ttatcaacct ggcgctggcg accttcatgc ccaatgaata catcacccat
     6421 ctggaagcaa cgcctgggat gctggtgcac attggcttat cgctcttttc ctatgccacg
     6481 ctaattatcg ccgccctgta cgcgctgcaa ctggcgtgga ttgattacca actgaagaac
     6541 aagaagctgg catttaacca ggaaatgccg ccattgatga gtatcgagcg taaaatgttc
     6601 cacatcacgc agattggcgt ggtgctgcta acgctcacgc tttgcactgg cctgttctac
     6661 atgcacaacc tatttagcat gggaaatatc gacaaggctg tgctctctat cgtggcgtgg
     6721 tttgtctata ttgtgctgct gtggggacat taccatgaag gatggcgtgg acgccgcgtc
     6781 gtctggttta acgttgcggg cgcggtaatt ctgacactgg cctacttcgg cagccgaatt
     6841 gtccagcagt taatcagcta aacccagaaa aggagtttcc cctggaacac atttctacta
     6901 ctacgttgat cattattctg atcatcatgg tggtcatttc agcctatttt tccgggtccg
     6961 aaaccggaat gatgaccctc aaccgctatc gtctgcgaca tatggcgaaa cagggtaatc
     7021 gctcggccaa acgcgtcgaa aaattgctgc gtaagccaga ccgcctgata agcctggtgt
     7081 taatcggcaa taacctggtc aatattcttg cctccgcgct cggcactatt gttgggatgc
     7141 gtttgtacgg cgatgcgggc gtggcaattg cgactggtgt gctgactttt gtcgtactgg
     7201 tatttgctga ggtattgccg aaaaccattg ccgcgctgta cccggaaaaa gtcgcttatc
     7261 cgagtagttt tctgctggct ccgctgctaa ttttgatgat gccgctggtc tggttgctga
     7321 atgctatcac ccgtatgctg atgcgcatga tgggtatcaa aaccgatatc gtggttagcg
     7381 gctctttgag caaagaagag ttgcgcacta tcgtgcacga atcacgctca caaatttccc
     7441 gtcgcaatca ggatatgctg ctgtcggtgc tcgatctgga aaaaatgacc gttgatgaca
     7501 tcatggtgcc gcgcagtgaa attatcggta ttgatatcaa cgatgactgg aaatcgattc
     7561 tgcgccaact ctcccactca cctcacgggc gcatcgtgct ctaccgtgat tcgctggacg
     7621 acgccatcag tatgctgcga gtacgtgaag cctggcggtt gatgtcggag aaaaaagagt
     7681 tcaccaaaga aaccatgctg cgcgccgcgg acgagatcta ttttgtgccg gaaggtacgc
     7741 cgctcagcac gcagttggta aagtttcagc gcaacaaaaa gaaagtcggc ctggtcgtca
     7801 acgagtatgg agacattcag gggctggtga cggttgaaga tattctggaa gagattgtcg
     7861 gcgatttcac tacgtcgatg tcgccaacac ttgccgaaga ggtcacgccg caaaacgacg
     7921 gttcggtgat tatcgatggc accgccaacg tgcgggaaat caacaaagcc tttaactggc
     7981 atctaccgga agatgatgcc cgcacggtta atggcgtcat tcttgaggca ctggaagaga
     8041 tccctgtcgc aggcacccgc gtgcgtattg gcgagtacga tatagatatt ctcgacgtac
     8101 aggacaatat gattaagcag gtaaaagttt ttcctgtgaa accgctgcgc gagagtgtgg
     8161 cggagtaacg aaaacggccc tgcattcgca tgcagggccg tgaattatta cgaaaacaga
     8221 aattacgctt ttgctttcgc tacagttacc atcgccgcac gaatcgtacg accattcagc
     8281 gtataaccct tctgcataat gcccagtacg ttacctggcg caacgtcatc agattccacc
     8341 attgcgatag cctgatgcac attcgggtcc agcggcacgt tagtttcggc gatcacttca
     8401 acaccaaact tacgcacaac atccagcatc gacttcagcg tcagctcaat gccttcaacc
     8461 atcgcagaca tatccgggtt agctttatca gccacttcca gcgcacgatc caggctatca
     8521 atcaccggca gcaattcgtt gatgaatttc tccagcgcga atttgtgggc tttttcaata
     8581 tccagttcag tacgtcgacg caggttttcc atttcggctt ttacacgcaa aatgccgtca
     8641 cgttcacggg tctgggcttc agccagctga gcttcgagat tcgcaatttt ttcatcgcgc
     8701 ggatccacct gctcagcaga agcttctggc tcaactgcct caatctcttc gtgctgatcc
     8761 atgataattt cttccggggc ttgcccctca ggcgttttct gttctttact actcatgaat
     8821 ttctccgcgt ttttttcgca ttcatctcgc taacttcgct tattatgggg atcagtttca
     8881 gggtttcaag ggaagcactc acattgtcat caatcttcgc aacaaggacc tcggaaaaat
     8941 gaataatcat ttcaagtgta ttggcattgt gggacaccca cggcacccca ctgcactgac
     9001 aacacatgaa atgctctacc gctggctgtg cacaaaaggt tacgaggtca tcgttgagca
     9061 acaaatcgct cacgaactgc aactgaagaa tgtgaaaact ggcacgctcg cggagattgg
     9121 gcaactagct gatctcgcgg tagtcgttgg tggcgacggt aatatgctgg gcgcggcacg
     9181 cacactcgcc cgttacgata ttaaagttat tggaatcaac cgtggcaacc tgggtttcct
     9241 gactgacctt gaccccgata acgcccagca acagttagcc gatgtgctgg aaggccacta
     9301 catcagcgag aaacgttttt tgctggaagc gcaagtctgt cagcaagatt gccagaaacg
     9361 catcagcacc gcgataaatg aagtggtgct tcatccaggc aaagtggcgc atatgattga
     9421 gttcgaagtg tatatcgacg agatctttgc gttttctcag cgatctgatg gactaattat
     9481 ttcgacgcca acaggctcca ccgcctattc cctctctgca ggcggtccta ttctgacccc
     9541 ctctctggat gcgattaccc tggtgcccat gttcccgcat acgttgtcag cacgaccact
     9601 ggtcataaac agcagcagca cgatccgtct gcgtttttcg catcgccgta acgacctgga
     9661 aatcagttgc gacagccaga tagcactgcc gattcaggaa ggtgaagatg tcctgattcg
     9721 tcgctgtgat taccatctga atctgattca tccgaaagat tacagttatt tcaacacatt
     9781 aagcaccaag ctcggctggt caaaaaaatt attctaattt tacgccagcc tctttactgt
     9841 atataaaacc agtttatact gtacacaata acagtaatgg tttttcatac aggaaaacga
     9901 ctatgttggc acaactgacc atcagcaact ttgctatcgt tcgtgagctt gagattgatt
     9961 ttcatagcgg catgaccgta ataactggcg agaccggcgc gggtaaatct attgcaatag
    10021 atgccctcgg tctttgtctc ggtggtcgcg ctgaagccga catggtgcgt accggcgctg
    10081 ctcgcgctga cctgtgcgcc cgtttttctc tgaaagatac gccagcggct ctgcgctggc
    10141 tggaagaaaa ccagcttgaa gacgggcatg aatgtttgct tcgtcgcgtg atcagcagcg
    10201 atggtcgctc ccgtggtttc atcaacggta cagctgttcc tctgtcacaa ctgcgcgaac
    10261 tgggtcagtt gctgattcag atccatggtc agcacgctca tcagttactc accaaacccg
    10321 agcaccaaaa gttcctgctt gatggctatg ccaatgaaac ctctctactc caggaaatga
    10381 cagcacgtta tcagttgtgg catcaaagct gccgtgacct cgcgcatcat caacagttaa
    10441 gtcaggaacg cgctgcccgt gcggaactgc tgcaatacca attaaaagaa cttaacgaat
    10501 ttaatccgca gcccggagag tttgaacaaa tcgacgaaga gtacaaacgt ctggcgaaca
    10561 gcggtcaatt gctgaccacc agccagaatg cattggcatt aatggccgac ggtgaagacg
    10621 caaacctgca aagtcagctt tacacggcta aacaactggt gagcgaattg attggcatgg
    10681 acagcaaact gtccggcgta cttgatatgc tggaagaagc taccatccag attgctgaag
    10741 ccagcgatga actgcgccac tactgcgatc gtctggatct cgatcccaac cgactatttg
    10801 aacttgaaca gcgcatctca aaacagattt cgctggcacg taaacatcac gtcagccctg
    10861 aggcattgcc acagtattac cagtcgctac tggaagaaca gcagcaactg gacgatcagg
    10921 ccgactcaca agaaacgctt gcgctggcgg taacgaaaca tcatcagcag gcactggaaa
    10981 tcgcgcgcgc attacaccaa caacgccagc aatatgcaga agaacttgca cagctgatca
    11041 ccgacagtat gcatgcgctc tcaatgccgc atgggcagtt tacgatcgat gttaaatttg
    11101 acgagcatca cctgggcgct gacggtgccg atcgtattga gtttcgggta accaccaacc
    11161 caggtcagcc aatgcagcct attgccaaag tcgcatccgg tggtgaattg tcccgcatcg
    11221 cactggcaat ccaggtcatc acggcgcgta aaatggaaac cccggcactg atttttgatg
    11281 aagtggatgt agggattagc ggtccaacag cggcagttgt cggcaaactg ctgcgtcaac
    11341 tcggcgaatc aactcaggtg atgtgtgtta cccacctgcc acaagtcgcg ggatgtggtc
    11401 atcaacacta ttttgtcagc aaagaaaccg atggtgcgat gacagaaacg catatgcaat
    11461 ccctgaataa aaaagcgcgg ttacaagagc tggcgcgcct gcttggtggc agtgaagtca
    11521 cacgtaatac actggcgaat gcgaaagaac tgcttgcagc gtaaactttt ttcctgcttc
    11581 acggtcagag taaacagcaa aacgccgtaa gaccggaaag caaaaggttt taaagtgatg
    11641 aaaggtctat tatcatcggc atattacaga tgagccacgt actgctcggg cccgaaaagg
    11701 aatcaaatca ctatgcgctg taaaacgctg actgctgcag cagcagtact attgatgttg
    11761 accgcaggct gttccactct ggagcgagtg gtttaccgtc ctgacatcaa ccaggggaac
    11821 tatctgaccg ctaacgacgt atccaaaata cgtgttggca tgacgcaaca acaagttgcg
    11881 tacgcattgg gtacaccgct gatgtccgat ccatttggta cgaatacctg gttctatgtc
    11941 ttccgccagc aaccaggtca tgaaggtgta actcagcaaa cgctgacgct gacctttaac
    12001 agtagcggtg tgttgaccaa tattgataac aaacctgcgc tgagtggtaa ctaataataa
    12061 agttgctctc aaagacgtta aaaaaggtgc tcaatgagca ccttttttct gtctgttatt
    12121 tattcgctga tttttctgct tgttgcctgc gaagctcttt cggatcggca atgagagggc
    12181 gataaatctc cacccgatcg ccatcatgca cactatcgct tagttttgcc ggacggctgt
    12241 aaatgccgac tttgttttta gttaaatcga tatcggtacg caattccagc aagccactgg
    12301 cgcgaatagc ttcttcaacc gtcgcgccct cctgcagcgt cactcgctgc aggtactgct
    12361 tctcaggtag cgcataagcc acctcaacgg caattttacc tggcactgta aacctctttc
    12421 gcacgaaccg taaaagcctg gaccatatta gccgccagct ctttaaacac gcgaccaaag
    12481 gcgagttcaa tcaacttatt ggtaaactca aagtcgagat gaaactcgat acgacacgcc
    12541 tcctggctca gcggcgtaaa cttccatcca ccaatcaatt tcttgaacgg cccatccacc
    12601 agattcataa gaatactttg gttactggtc aactggttgc gggtagtaaa cgttttgctg
    12661 atcccagcct tagagacatc taccgccgca gtcatctgcc caggagtgga ctccagaatc
    12721 cgacttccgg tacaacccgg caaaaactga ggataagact gaacgtcatt cactaactga
    12781 tacatttgct ccgcgctgta gggtaccagt gcggtccggc taatctgagg cataacaatt
    12841 tccatcaaca aaaatccaac aaataatatc atttatcctg ctgtaaaaaa aacgctatcc
    12901 cggcgctggg taacatcggg ttcatgctaa gatagagcct tgtcccccgc aggattgata
    12961 tggggtgttt tcgatttcag attaccgatg attcacgacg cttatgacga agaaaaaagc
    13021 acataaactt ggttcagcga ccatcgcgct taacaagcgc gcccgtcacg aatactttat
    13081 cgaagaagag ttcgaagcgg gacttgccct gcaaggctgg gaggttaaat ccctgcgcgc
    13141 aggaaaagcc aatatcagcg acagctacgt ccttctgcgt gacggagagg catttctgtt
    13201 tggcgctaac atcacgccaa tggccgtggc ctccacgcat gtggtgtgcg atcctacccg
    13261 tacccgcaag ttacttctca accagcgcga actggactca ttgtacggtc gcgtcaatcg
    13321 agaaggctat accgtagtgg cgctctccct atactggaaa aatgcctggt gcaaagtgaa
    13381 aatcggcgtc gccaaaggta agaaacagca cgataaacgt tcagacatca aagaacgcga
    13441 atggcaggtg gataaagcgc gtatcatgaa aaacgcccac cgttaaacct gtagtccaat
    13501 tattgaccag ttcctcaccg cgcctccctc tccggcggcg cgaatgaaca tcttattggc
    13561 tatcacatcc gacacaaatg ttgccatccc attgcttaat cgaataaaaa tcaggctaca
    13621 tgggtgctaa atctttaatg ataacgccat tgaggctggt catggcgctc ataaatctgg
    13681 tatacttacc gttacacatt ggggctgatt ctggattcga cgggatttgc gaaacccaag
    13741 gtgcatgccg aggggcggtt ggcctcgtaa aaagccgcaa aaaatagtcg caaacgacga
    13801 aaactacgct ttagcagctt aataacctgc ttagagccct ctctccctag cctccgctct
    13861 taggacgggg atcaagagag gtcaaaccca aaagagatcg cgtggaagcc ctgcctgggg
    13921 ttgaagcgtt aaaacttaat caggctagtt tgttagtggc gtgtccgtcc gcagctggca
    13981 agcgaatgta aagactgact aagcatgtag taccgaggat gtaggaattt cggacgcggg
    14041 ttcaactccc gccagctcca ccacttttta gtaaaatgac gtctactcaa gtctactaag
    14101 gccgcattcc acaagggttt gcggcctttt tcatgtccag tgaagtctac tgagaattgc
    14161 tagaaaccac tccttatggc accctcctat tatccctcgt cgatgaggga gtaaaaacga
    14221 gggagcaaaa atgccgaagg tagcaacaaa actcactgac acagagatca aaaaggccaa
    14281 gcccacagag aaagagttca cgctatggga tggagacggc ttgttcctgc gtatcaaacc
    14341 aagtggtaag aaaatctggc atctagccta tgccgctcca gtgtcaaaga agcgctcgaa
    14401 aatgagtctt ggattttatc cgcacttaac gcttgctcaa gcaagagctt tgcgcgacga
    14461 atatttatct ttgctagcga atggtataga tccacaatct cataacgaac aaaaagctaa
    14521 cgcgctaaaa gatgcggctg aacacacact gcagacagtt gcgaagcagt ggcttgaaga
    14581 gaaggtcaaa acttccggca tctcagaaga tcacgcaaaa gacatctggc gcagccttga
    14641 aaaaaacatc ttccccggtc ttgggcatct tccaattaaa gaaatacgtc caaagctttt
    14701 aaaacagcat cttgacccta tagaacagag aggtgtgctt gagacgctac gccgcatcat
    14761 ttcccgacta aatgaaatat tccgctgggc agcaacagaa gaactcatcg agttcaaccc
    14821 agcggataac ctcggtcacc gtttcagtaa gcctaagaag caaaatatgc cagctctacc
    14881 tccaaatgag ctaccgcgat tcatgattgc tttatctaac gcctctatcc gtcttgagac
    14941 gcgtctgctg attgaatggc agttgttgac ttgggttcgt cccggtgaag ccgttcgggc
    15001 gcgttgggga gatattgatg taaagaataa attttggaac atcccgccag agttcatgaa
    15061 gatgaaacgg cctcataaag ttccgcttag caaagaagca ctacgcatcc tcgaaaaaat
    15121 gcagccaatc agcgggcatc gtgagtgggt tttccccagc atcaaagcac ctcttaatca
    15181 tatgcatgag caaacagcta atgccgccat aattagaatg ggattcggag gtgaactggt
    15241 tgctcacggt atgcgttcta tagccagaac agccgcagag gaatctggta agttcagaac
    15301 agaagttctt gaggcagctc tcgctcattc aaaaaaagat gaaattatcg ctgcttataa
    15361 ccgtgcggaa tatttagttg aacgtgccga gctaatgcaa tggtggagtg attacgttca
    15421 agcacaaaaa tataagttta tggttgctta agatggatga tacctaactt aacatattat
    15481 gttataaaaa tcctctcaca ctgatgatta atcatcagtg gagatctttc aaagtattta
    15541 tatacttctg aacaccttcg ttatctatga accctttccc attaccatta atataagaaa
    15601 tactcttcca gtccatataa acagacatta tagaaaccaa attaactaac tttacaggca
    15661 tcatattaac aaaaatatca agataatagt ctcggtgttc ttctgggcac ttttccttta
    15721 caactttgaa aacaacatac acacacctta ccattgaaaa tatagacata gaagactcat
    15781 tatctatgtt atctataaaa cgtctaacct caataacttc gcattctcca ttatcactta
    15841 tctgtgcttg aataaaatcc catatagctt gctcgatttc cgagaccgca ttagatgact
    15901 taaactcagt attatttata gttatagaaa aagagctaaa ctcttcccgc tccgaagata
    15961 tgagattata aaagctatcc tcaaacctct ttaacatgag atctttttct tgccttcttt
    16021 catctttaat caatatttcg ttagcttccc tctgtaattt tagagagttt ataagcataa
    16081 caatagttat gaatgtgaga aggggattta atactccacc aacgaaatca ccgaaagttc
    16141 cccaatcccc tttatcatca gaaaacccca tccacttctc tttattaagg agatctaaag
    16201 ggttatccca atgattaaag tgagaaaaat acaaatacca aacaaccaat attattaaaa
    16261 cagtaatcag tttcaaggta gacatcttca tttaaaggac ttcttgtata atgtaagttt
    16321 taagttcgcc tatgtgattt cttaattcaa tagcagagat agttattggt atgaaagcat
    16381 ctctaccgcc cctgttgtta acatcaagat tccttgaaaa tttgtttttt tggctttgaa
    16441 gaacatgctc ccaatttaaa gcatgcctaa aagcaataga atcagattgc ctgtcaagta
    16501 aaagcctacc tgttccagtt aacatattaa atcttgttat tatgacattt tcttgtactg
    16561 gttgaggaga ctcatgttca atatttatat tatataaagt agccgaatcc attttagcta
    16621 cgataatttt cctttgtgga gttctccttc ttaataggac attataccct tgtttatcaa
    16681 cagtgcgatg catgttttca ataggatcct gaatcctatt caggagagtt ctctctaaag
    16741 gttcaagctc atttacaaga tctatcgaag cttgattagt tagattatga acctctccca
    16801 tccctttgga gatgtaataa gacataacat caaaaaaaac agttttccca atttcgttaa
    16861 atctggttat tttttctgga tcttctatcc tgacacaaaa tttctgtcca aaagaaccat
    16921 cgaaagtatg tttaaaaata ttcctaagcc catgtataga tgttgatttt ttaggaaccg
    16981 aatcatctaa tattgtgctt gctgtaattt gtgttatatc cgaagcccca ctgaaaactt
    17041 ttatgccaga agtcatttct agctcttcaa aatcatcttg cccatgaccg actacaacat
    17101 ccatatctag attaatcatt gaagttctct ttatgtgttg atgttgagtg cttttcactc
    17161 agaaatatct taattatgtc taaaaatttc aatcatgcct atatttatta caaaaaattt
    17221 ttccgcagcc ttaccttgcc tccaaaagga cgcaaccaaa tgattaataa caataaaaat
    17281 aagataaccg agcgtcgcag gcaggatggc tcaccaagtg caccatccca caacgcgaag
    17341 gatatgtgat ctgaccgtcg tcaagaacaa tagcggaacc ctcaaaaggg aacccccgct
    17401 attcttcttg acgtcctaaa tattggagtt gtcatttagt cttactgaag agattgcggc
    17461 gttccatgaa ta
//
LOCUS       DAJOSY010000090        17315 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJOSY010000090 DAJOSY010000000
VERSION     DAJOSY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.090"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            20..1255
                     /gene="moeA"
                     /locus_tag="OHN27_003244"
     CDS             20..1255
                     /gene="moeA"
                     /locus_tag="OHN27_003244"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCQ2287630.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVVGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            1255..2004
                     /gene="moeB"
                     /locus_tag="OHN27_003245"
     CDS             1255..2004
                     /gene="moeB"
                     /locus_tag="OHN27_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCQ2287631.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(2183..2845)
                     /gene="fsa"
                     /locus_tag="OHN27_003246"
     CDS             complement(2183..2845)
                     /gene="fsa"
                     /locus_tag="OHN27_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCQ2287632.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            2976..3875
                     /locus_tag="OHN27_003247"
     CDS             2976..3875
                     /locus_tag="OHN27_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCQ2287633.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            3881..6313
                     /locus_tag="OHN27_003248"
     CDS             3881..6313
                     /locus_tag="OHN27_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCQ2287634.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGHDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            6459..7274
                     /gene="ybiV"
                     /locus_tag="OHN27_003249"
     CDS             6459..7274
                     /gene="ybiV"
                     /locus_tag="OHN27_003249"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCQ2287635.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHVALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            7426..8691
                     /locus_tag="OHN27_003250"
     CDS             7426..8691
                     /locus_tag="OHN27_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCQ2287636.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(8932..10524)
                     /locus_tag="OHN27_003251"
     CDS             complement(8932..10524)
                     /locus_tag="OHN27_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCQ2287637.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            10743..11663
                     /gene="ldtB"
                     /locus_tag="OHN27_003252"
     CDS             10743..11663
                     /gene="ldtB"
                     /locus_tag="OHN27_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCQ2287638.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(11722..12840)
                     /locus_tag="OHN27_003253"
     CDS             complement(11722..12840)
                     /locus_tag="OHN27_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCQ2287639.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(12837..13304)
                     /gene="mntR"
                     /locus_tag="OHN27_003254"
     CDS             complement(12837..13304)
                     /gene="mntR"
                     /locus_tag="OHN27_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCQ2287640.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            13490..13618
                     /gene="mntS"
                     /locus_tag="OHN27_003255"
     CDS             13490..13618
                     /gene="mntS"
                     /locus_tag="OHN27_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCQ2287641.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            13890..15473
                     /gene="opgE"
                     /locus_tag="OHN27_003256"
     CDS             13890..15473
                     /gene="opgE"
                     /locus_tag="OHN27_003256"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCQ2287642.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(15522..16037)
                     /gene="ompX"
                     /locus_tag="OHN27_003257"
     CDS             complement(15522..16037)
                     /gene="ompX"
                     /locus_tag="OHN27_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCQ2287643.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(16090..16155)
                     /locus_tag="OHN27_003258"
     CDS             complement(16090..16155)
                     /locus_tag="OHN27_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287644.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            16390..17277
                     /gene="rhtA"
                     /locus_tag="OHN27_003259"
     CDS             16390..17277
                     /gene="rhtA"
                     /locus_tag="OHN27_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCQ2287645.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         4184 a         4617 c         4393 g         4121 t
ORIGIN      
        1 cctccgcagg agtgttttca tggaatttac caccggattg atgtcgctcg acaccgcgct
       61 taatgagatg ctttctcgcg tcaccccact gaccgcccag gaaacgctgc cactggtaca
      121 gtgttttggt cgtattctgg cgagcgatgt cgtttcgccg cttgatgttc cggggtttga
      181 taactccgca atggacggct acgcggtgcg tttagccgat attgcctccg ggcaaccgct
      241 gcccgttgtc ggtaaatcct ttgccggtca gccataccat ggtgaatggc ctgcgggtac
      301 ctgcattcgt attatgaccg gtgcgccggt gccggaaggc tgcgaagcgg tggtgatgca
      361 ggagcagact gaacaaacgg acaatggcgt gcgttttact gctgaagtgc gtagcgggca
      421 aaatattcgc cgtcgcggtg aagatatctc tgcaggtgcg gttgttttcc cggcgggaac
      481 tcgcctgact accgcagagc tgccagtaat tgcttcactg gggattgccg aagttccggt
      541 gattcgtaaa gtgcgtgtag cgcttttttc taccggtgat gaactccagt tgcccggtca
      601 gccactgggc gatggccaaa tctacgatac caaccgtctc gccgtacacc tgatgttaga
      661 acagctgggt tgcgaggtaa tcaacttagg gattatccgc gacgatcccc atgccctgcg
      721 cgccgcattt attgaagccg acagccaggc ggatgtagtg atcagttccg gcggtgtttc
      781 agtgggtgaa gcggattaca ccaaaacgat tcttgaagag ctgggggaga tcgccttctg
      841 gaagctggcg attaaaccag gtaaaccgtt cgcgttcggt aaactcagca atagctggtt
      901 ctgcggcctg ccgggcaacc cggtttcagc gacgctgacc ttctatcaac tggtacagcc
      961 tttgctggca aaactaagcg gcaacaccgc cagcggcctg cccgcgcgcc agcgcgtacg
     1021 cacagcgtcc cgcctgaaaa aaacgccagg acgtcttgat ttccagcgcg gcgtgctgca
     1081 ccgcaacgcc gatggcgaac tggaagtgac gaccaccgga catcagggct cacatatatt
     1141 cagctccttt agcctcggca actgctttat cgtactggaa cgcgatcgcg gcaatgtgga
     1201 agtgggcgaa tgggtggaag tagaaccgtt taacgcgttg ttcggaggcc tgtaatggcg
     1261 gaactcagcg atcaggagat gctgcgctac aaccggcaaa tcattctgcg cggttttgat
     1321 tttgacggtc aggaggcgct aaaagattct cgtgtgctgg tagtcggcct cggtggactc
     1381 ggctgcgcag cttcgcagta tctggcaagc gccggtgtcg gcaacctgac gctgctcgac
     1441 ttcgactcgg tttcgctctc gaatctgcaa cgccagacgc tgcacagtga tgccacggtc
     1501 gggcaaccga aggtggaatc cgcccgtgac gccctggcgc ggatcaatcc acatatcgcg
     1561 attacgccag tcaatgcact gctggatgac gcagaacttg cagcattgat tgctgaacac
     1621 gatctggtgc tcgactgtac ggataacgtt gcggtacgta atcaactgaa cgcaggctgt
     1681 tttgccgcga aggtgccgct ggtttccggc gcggcaattc gtatggaagg gcaaatcacg
     1741 gtcttcactt atcaggacgg cgaaccgtgc tatcgctgcc ttagccgttt gtttggcgag
     1801 aacgcattaa cctgcgtaga agcaggcgtg atggcaccgt tgatcggcgt aattggttcg
     1861 ttgcaggcaa tggaagcgat caaactgctg gcaggttatg gcaaaccggc cagcgggaaa
     1921 atcgtcatgt acgatgcgat gacctgtcag tttcgcgaaa tgaaactgat gcgcaatccg
     1981 gggtgtgagg tgtgcgggca gtaattgcca cctccaggcc tgataagacg cgacaagcgt
     2041 cgcatcaggc atttgctcca gacgccagat gcggcgtgaa cgccttatct ggcctacggt
     2101 tcgggcacag atttccaagc gcggcaggca tcggtgcact ccgccacgtc atcagcaatt
     2161 tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc tgccagtcct
     2221 gctcaaactt cgccacagcg gcttccaccg ccggatagct aatcatctgt tgtgccacat
     2281 ccagtggcag agtaattgat tcacatcctg ccagtaagca gtccagcgcc tgacgcgggg
     2341 ttttgaaact cgctgccagc actttcgcct gcggcgcatg cattttcaat aactggtgta
     2401 agtcggtcac agtctgaatg ccgctaccgc cctgagcatc aatacgatta acgtaaggcg
     2461 caacatattc cgcacctgcc agcgccgaca gcagcccttg tgctgcgcca tataccgcgg
     2521 ttcccagcgt tggaatccct tccgctttta acatcttaat agctgccagc ccctcggcgg
     2581 tcaccggaac tttcaccacg atatccgcaa taatagaacg caatttacgt gcgtcattca
     2641 ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga ccgcccatcg
     2701 cttcatgaag ttgcggaagc acaacatcca gcggtttttt acccgcggcg ataatgcttg
     2761 ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgccttcacc gcaacaacgt
     2821 ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg tctgaatgta
     2881 gactatcacg ggaaaactct ctgaccagtt gacgcacatc aatctaactt tcattcgaaa
     2941 gtattttaat gtttgaacga aacagagagg caactatgat tttcaatatt cagcgctact
     3001 cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt tcgctgggct
     3061 gccgctggtg tcagaacccg gaaagccgcg cccgcacgca ggatctgctg tatgacgcac
     3121 gactgtgtct ggaaggctgc gagctgtgcg ctaaggccgc gccggaagtg attgagcgcg
     3181 cgctgaatgg tttgcttatt catcgggaaa agttaacccc ggagcatctg acggcgttaa
     3241 ccgactgctg tccgacacag gcattaaccg tgtgtggtga agtgaaaagc gttgaggaga
     3301 tcatgacgac cgttctgcgc gataaaccgt tttacgatcg cagcggcggc ggtttaacgc
     3361 tttcgggtgg tgaacccttt atgcagccgg aaatggcgat ggcgctactg caagccagcc
     3421 acgaggcagg cattcatact gcggtagaaa cctgtctgca tgtgccgtgg aaatatatcg
     3481 ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacgtt gccgacgcgc
     3541 cgtttaaaca gtggaccgac ggtaacgccg ccagagtgct ggataacctg aaaaaactcg
     3601 ccgcagcggg caaaaaaatc attatccgcg tgccgctgat tcagggcttt aatgccgacg
     3661 aaacctctgt aaaagctatt accgattttg ccgccgacga gctgcacgtt ggcgaaattc
     3721 atttcctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat ctgccctatg
     3781 acgccccgga aaaaccgctt gatgcgccag aactgctcga ctttgcccag cagtatgcct
     3841 gccagaaagg gttaaccgcg accttacgag gataacaacc atgaccacac tgaaactgga
     3901 cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg tgaaaccgcc
     3961 agtctgtacc gagcgcgcgc agcactatac cgagatgtat caacaacatc tcgataagcc
     4021 gatcccggta cgtcgcgcgc tggcactggc gcatcacctg gcgaatcgca ccatctggat
     4081 caaacacgat gagttgatca ttggcaacca ggcaagcgaa gttcgcgccg cgccgatctt
     4141 cccggaatat actgtctcgt ggatcgaaaa agagattgat gatctggcag atcgtcccgg
     4201 tgctggcttt gcggtgagcg aagagaacaa acgcgttctg catgaagtgt gcccgtggtg
     4261 gcgcggtcag accgtacagg atcgctgcta cggcatgttt accgatgagc aaaaaggtct
     4321 gctggcgacc ggaatcatta aagcggaagg caatatgacc tccggcgatg cgcacctggc
     4381 ggtgaatttc ccgctgctgc tggaaaaagg gcttgatggt ctgcgcgaga aagtagcgga
     4441 acgtcgctcg cgcatcaacc tgacggtgct ggaagattta cacggtgagc aattcctgaa
     4501 agcgattgat atcgtgctgg tggcagtcag tgaacacatt gaacgtttcg ctgccctggc
     4561 gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc gatgaactgc tggcgatggc
     4621 agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg cgctgcaact
     4681 gtgttacttc atccagttga ttttgcagat cgaatctaac ggtcactcag tatcgtttgg
     4741 tcgtatggac cagtatctct acccgtacta tcgccgcgac gttgaactca accagacgct
     4801 ggatcgcgaa cacgccatcg agatgctgca tagctgctgg ctgaaactgc tggaagtgaa
     4861 caagatccgc tccggctcac actcaaaagc ctctgcggga agtccgctgt atcagaacgt
     4921 cactattggc gggcaaaatc tggttgatgg tcaaccaatg gacgcggtga atccactctc
     4981 ttacgcgatc ctcgaatcct gcggtcgcct gcgttccact cagcctaacc tcagcgtgcg
     5041 ttaccatgca ggaatgagca acgatttcct cgacgcctgc gtacaggtga tccgttgcgg
     5101 cttcgggatg ccggcgttca acaacgacga aatcgtgatc ccggaattta ttaaactcgg
     5161 tattgaaccg caggacgctt atgactacgc agcaattggt tgtatagaaa ccgccgtcgg
     5221 tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc gcgtgatgct
     5281 ggcggcgctg gaaggcgggc atgatgccac cagcggcaaa gtgttcctgc cacaagaaaa
     5341 agcgttgtcg gcaggtaact tcaacaactt cgatgaagtg atggacgcgt gggatacgca
     5401 aatccgttac tacacccgca aatcaatcga aatcgaatat gtcgtcgaca ccatgctgga
     5461 agagaacgtg cacgatattc tctgctcggc gctggtggat gactgtattg agcgagcgaa
     5521 aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg tcggcattgc
     5581 caacctcggc aacagcctgg cggcagtgaa gaaactggtg tttgaacaag gtgcgattgg
     5641 tcagcaacag cttgctgccg cactggcaga tgacttcgac ggcctgactc acgagcagct
     5701 gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata ctgtcgatac
     5761 gctgctggct cgcgcttatc agacctatat cgacgaactg aaacagtacc ataatccgcg
     5821 ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt acgtcatcaa tctccgctaa
     5881 cgtaccgttt ggcgcgcaga ctatggcaac accggacggg cgtaaagccc acaccccgct
     5941 ggcagaaggc gcaagcccgg cctccggtac tgaccatctt ggccctactg cggtcattgg
     6001 ctcagtgggt aaactgccta cggcagcgat tctcggcggc gtgttgctca accagaaact
     6061 gaatccggca acgctggaga acgaatctga caagcagaaa ctgatgatcc tgctgcgtac
     6121 cttctttgaa gtgcataaag gctggcatat tcagtacaac atcgtttccc gcgaaacgct
     6181 gctggatgcg aaaaaacatc ccgatcagta tcgcgatctg gtagtgcgtg tcgcgggcta
     6241 ttccgcgttc ttcaccgcgc tctctccaga cgctcaggac gatatcatcg cccgtactga
     6301 acatatgctg taatctcctc ggcccggcgg cgtcgtcgcc gggctaaatc attcacatca
     6361 tcaatttcat ccttactttc attcgaaata taatttgtgc tctgcgtcac attgttatta
     6421 cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa gttatcgtca
     6481 cagacatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca cgttttatgg
     6541 cgcaatatca ggaactgaaa aagcgcggca ttaagttcgt tgttgccagc ggtaatcagt
     6601 attaccagct tatttcattc tttcctgagc taaaggatga gatctctttt gtcgcggaaa
     6661 acggcgcact ggtttacgaa catggcaagc agttgttcca cggcgaactg acccgacatg
     6721 aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt gtcgcctgcg
     6781 gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca ctgatggcaa
     6841 aacactacca tcgcctgaaa cctgtaaaag attatcagga gattgacgac gtactgttca
     6901 agttttcgct caacctgccg gatgaacaaa tcccgttagt gatcgacaaa ctgcacgtag
     6961 cgctcgatgg cattatgaaa cccgttacca gtggttttgg ctttatcgac ctgattattc
     7021 ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat ctgtcaccgc
     7081 aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa atggcgcgtt
     7141 attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt tacgctaccg
     7201 atgataataa tcatgaaggc gcgctgaatg tgattcaggc ggtgctggat aacacatccc
     7261 cttttaacag ctgacctctc taccggagcc gtgcgctccg gttttccctt ctttttacat
     7321 cctgtacatc acatcaaatc attaaacttg cattaactta tttataactt atattgtcac
     7381 atgcaaatca gaatggtttc gaatgaaagt taatgagagg tggttatggc ttctactttt
     7441 accagcgaca cattgcctgc cgatcacaaa gcagctatcc gtcagatgaa gcacgcgctg
     7501 cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc taagcgatga cattgccacg
     7561 cgagtggctg aaatcaacgc actcaaagca cagggcgatg ccgtctggcc ggtgctgtct
     7621 tatgccgata tcaaagcagg tcatgttact gcagagcagc gcgaacagat taaacgtcgc
     7681 ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag cgctaggctg ggatcagtcg
     7741 atgctggact atctggaccg caaccgcttt gacgaggtct acaaaggccc cggcgataat
     7801 ttcttcggga cgctcagcgc ttcacgtccc gagatttacc ccatctactg gtcgcaggcg
     7861 caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct caatcgtctg
     7921 tggacatttg aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt catctaccct
     7981 gaccgtatcc gccgccgtcc gcccggaacg acctccaaag gtcttggagc gcataccgac
     8041 tccggggcac tggaacgctg gctgcttcca gcgtatcagc gcgttttcgc caacgtcttt
     8101 aatggcaatc tggcgcaata tgatccctgg catgcggcac atcgtacgga agttgaagag
     8161 tacacggtgg acaacaccac caaatgttcc gtgtttcgga cattccaggg ctggacagcg
     8221 ctctctgata tgctgcctgg tcaggggctg ctgcacgtcg tgcccattcc tgaagctatg
     8281 gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg aggatgaact gtgcggcgta
     8341 gcgcccggaa gagtattgcc ggtatcagag caatggcatc cactgttgat tgaggcgtta
     8401 accagcattc caaaactcga agccggagac tccgtctggt ggcactgcga cgtcatccat
     8461 tccgttgccc ccgttgaaaa tcaacaaggt tggggcaacg tgatgtacat tcctgcggca
     8521 ccgatgtgcg agaaaaatct tgcctacgcg cacaaggtga aggccgcact ggaaaaaggc
     8581 gcatcgccgg gcgacttccc gcgcgaggac tatgaaacaa actgggaagg acgctttacg
     8641 cttgccgacc tcaacattca cggtaagcga gcgttgggca tggatgtttg agaattaatg
     8701 gaagtgaatt gatgtgggat taagaaaatc gattcgacgt tactcagatt gctgacaaat
     8761 gcgctttgtt aatgcctgat gcggcgttaa cgccttatcc ggcctacaaa gtcctgcaga
     8821 ttcaatatat tgcaggtact gcgtaggcct gataagcgta gcgcatcagg ttattttgca
     8881 tttgtcatca gccgccgtca ccccgacagt atcggggtga cggataatca cttactcgat
     8941 ccctttacta cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc gttccggggt
     9001 gatttccaga atgcgggtcg ccagggagct tacgaactca cggtcgtgtg aaacaaagat
     9061 cagcgtgccc tgatacagtt ccagtgccat gttcagcgac tcaatggatt ccatatccag
     9121 gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta acttaccaaa
     9181 cagcatccgc cctttttccc caccggaaag cactttagct ggctttttga tgtcgtcctg
     9241 gctgaacagc aaacgaccga gaatactgcg taccgcctgc tcgtcatcgc cttcctgctt
     9301 ccactggctc atccattcga acacggtcag atcattttca aactcatatt cgtggtcctg
     9361 agcatagtaa ccaatgcgcg cgttctcaga ccattttacg gtgccgctgt ccggttgcag
     9421 atcgcccacc agcgttttca gcagcgttga tttaccgacg ccgttggtac ccagtaccgc
     9481 cagtttttca cccacttcca gcagcaggtt gagattttta aacagcggac cgttatcaaa
     9541 ccctttggtc agaccttcca cttccagcgc gttacggaac agtttcttat cctgttcaaa
     9601 acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga ttttatcaat
     9661 ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa agcggctaac
     9721 gaaagattgc aactcagcaa tctgcgcttt cttcttggcg ttatcggcca gcagacgttc
     9781 acgcgcctgg gtcgccgccg tcatgtactc atcgtagtta cccggataaa cgcgcagctc
     9841 gccgtaatcc agatccgcca tgtgggtaca gaccatgtta aggaagtgac ggtcgtgcga
     9901 gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc gaatggtgtc
     9961 gatgtcgagg ttgttggtcg gttcgtcgag caggagaata tccggatcag caaacagcgc
    10021 ctgcgccaga agcacacgca gcttccagcc aggagcaact tcactcatcg ggccgtagtg
    10081 ctgttccact ggaattccca cgccaagcaa cagttcaccg gcgcgagctt ccgcagagta
    10141 accgtccatt tcgccgtatt taacttccag atcggccact ttatagccgt cttcttcact
    10201 catttccggc aaagcataga tgcggtcgcg ctcctgcttc acttcccaca actctttatg
    10261 ccccatgatc accgtatcca gcacagtgaa ctcttcaaag gcaaactgat cctgacgcag
    10321 tttaccaatg cgctcgttgg gatcgaggga aacgttaccc agcgtcggct caaggtcgcc
    10381 gccgaggatc ttcataaagg tggatttacc actaccgttc gcgccaatca ggccgtaacg
    10441 gttgccgccg ccaaatttga cggaaatgtt ttcaaacaac ggcttactgc cgaactgcat
    10501 ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac aaaccgcgca
    10561 ttatgccata agtctgaaag aagatcctgc tcctcgctta aggtctacac tcaagccaaa
    10621 ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg aacttataat
    10681 gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa atctcgctta
    10741 acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc ggcttttgca
    10801 gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg gttggtcaga
    10861 atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt gccgccgagt
    10921 accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc ttcctgccga
    10981 aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc gttcatgaag
    11041 gcatcgtcat taacagtgct gagatgcgtc tttattacta tccgaaaggg accaacaccg
    11101 ttatcgtgct gccgatcggc attggtcagt taggcaaaga tacgcctatc aactggacca
    11161 ccaaagttga gcgtaaaaaa gcaggcccga cctggacgcc gaccgccaaa atgcacgcag
    11221 agtaccgcgc tgcgggcgaa ccgcttccgg ctgtcgttcc ggcaggtccg gataacccga
    11281 tggggctgta tgcactctat atcggtcgcc tgtatgctat ccatggcacc aacgccaact
    11341 tcggtatcgg cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa gacatcaaat
    11401 tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag ccggtaaaag
    11461 cgaccaccga gccagacggc agccgttata ttgaagtcca taacccgctg tctaccaccg
    11521 aagcccagtt tgaaggtcag gaaattgtgc caattaccct gacgaagagc gtgcagacag
    11581 tgaccggtca gccagatgtt gaccaggttg ttcttgatga agcgattaaa aaccgctccg
    11641 ggatgccggt tcgtctgaat taatcttcaa aacttaaagc aaaaggcgga ctcataatcc
    11701 gcctttttta tttgccagac cctagttggc cgggagtata actaacaaaa catatcccac
    11761 caacgccgcc cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg
    11821 atcgttcgcc atacgtagcg caatcaaatt tgccagcgat ccgggtaata acccaaagcc
    11881 acctacgttt accgcccata ccagtaataa agacggcggc acatagttca gcaacaatat
    11941 ggtactcggc acgttactga tcacctgcga taaaccgatt gccgttaacc ataacccggg
    12001 ttcagataga tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa
    12061 atggacgtcg ataaacatcg ccataaacac cagcagcagc gtccagtcca cactgagcac
    12121 cacgcgacgt gccagcagcg caaagcctgc cgccacaatc accagtcccc acagctcttg
    12181 tttgaactcc agcgccgtca gaaagacgat atacagcccc agacaactcc acaccagccg
    12241 cggtttccac tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca
    12301 acaccagcac aggagcagga gcgtcagcat cattgcgcca gccagcggtg ccatttgggc
    12361 aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc
    12421 aattggcgtc agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt
    12481 aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc
    12541 gacatcgttg gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag
    12601 ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct
    12661 taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc
    12721 gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac
    12781 aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat
    12841 ttggcaccgt gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg
    12901 tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag
    12961 aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc
    13021 gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta
    13081 agcattttag ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga
    13141 gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg
    13201 cgccgatgcg cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac
    13261 tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct tcctcattat
    13321 ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt
    13381 acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta
    13441 atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt
    13501 caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc
    13561 tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttccg ataaatagcg
    13621 gcgtcgcggt acgccgcttc actcctgctt tcatgcaggc ataacgcgtt ttggtctgaa
    13681 aaaccccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt
    13741 gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat
    13801 gcggacccct ttccactctt cacgcactct tgcaggtatt gacccttgac gccagggtaa
    13861 gcacatggcg tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta
    13921 cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta
    13981 acctcggctt aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc
    14041 ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg
    14101 tggcggcttg ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc
    14161 tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt
    14221 acagctacct ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa
    14281 aagagaatga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg
    14341 cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag
    14401 atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga
    14461 ttgaagagca agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg
    14521 ccgttaagcc gaagtatcag acctatgtgg tggtgatcgg tgaaagcgcg cgtcgcgatg
    14581 ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt
    14641 tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt ggcttaacgc
    14701 tcaatcgcgt tgtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc
    14761 gcgcgggctt ccagacctgg tggttttcca accagggtca aatcggcgaa tacgataccg
    14821 ctatcgccag catcgccaaa cgagcagatg aagtgtactt cctgaaagaa ggtaattttg
    14881 aagcagataa aaacaccaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc
    14941 aagagcactc gcaaccgcag ctgattgttc tacatctgat gggctcacat ccgcaggcct
    15001 gcgacaggac acaaggaaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc
    15061 tctataccat gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca
    15121 gcggcagcag cttctcgctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg
    15181 gtaaagacgt gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt
    15241 ttatggtcat ttccagcgac gataaagcgc atcgtgtgat taaagcccgc cgctcagcca
    15301 atgacttctt aggctttttc tcccagtgga cggggattaa agcgaaggaa attaacatca
    15361 aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac
    15421 agaaggtgga ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa
    15481 aatccgcccc gagaggcgga ttttttatat caccaaagtg attagaagcg gtaaccaaca
    15541 ccggcaatcc aggtgcctac gtcaacgcta cgaatacggc tctgctcgta agagaagtcc
    15601 agagcaacgt tttccatcgg gttgaactgc agacccgcac cgtaggagaa accgtagtcg
    15661 ctggtgtcgt gtttgtaggt cgggtattca gtggtctgga atttaccata acccacaccc
    15721 actacaccgt agatgcttgc ccagtcgtta atgcggtaag ccggaccagc agtgatgccg
    15781 tagtactggt ttttgttgta gtcaccagag cttgcagtac ggcttttctc ggtgtaagtg
    15841 aaagaaccga tcacacccag cgggctgttg tcttcttcat agcggtattt caggttgaaa
    15901 ccgcccattt tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc agttacagta
    15961 gaagtcgcag ctacggaagt acctgcggtg aaagccagaa ctgcggccag tgctgaaaga
    16021 catgcaattt ttttcataac cacctcaaat gtgattcaaa taagtcctaa gttttaaata
    16081 tatcaaaaat taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc atttcatgta
    16141 acagaacgtt tccatacacc gctatccatc taaatttaaa tcactttttc agagaactgc
    16201 gtaagtatta cgcatgtttt ccctgtcatt catccagatt attcctaatc accagactaa
    16261 tgattccatc aatcctggcg cattttagtc aaaacggggg aaaatttttt caacaaatgc
    16321 tcaaccagca ttgggtatat ccagtacact ccacgcttta cttaagtcta gatatttgtg
    16381 ggagaaagga tgcctggttc attacgtaaa atgccggtct ggttaccaat agtcatattg
    16441 ctcgttgcca tggcgtctat tcagggtgga gcctcgttag ctaagtcact ttttcctctg
    16501 gtgggcgcac cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat cctcatcgcg
    16561 ttctttaagc catggcgact gcgctttgcc aaagagcaac ggttaccgct gttgttttac
    16621 ggcgtttcgc tgggtgggat gaattatctt ttttatcttt ctattcagac agtaccgctg
    16681 ggtattgcgg tggcgctgga gttcaccgga ccactggcgg tggcgctgtt ctcttctcgt
    16741 cgcccggtag atttcgtctg ggttgtgctg gcggttcttg gtctgtggtt cctgctaccg
    16801 ctggggcaag acgtttccca tgtcgattta accggctgtg cgctggcact gggggccggg
    16861 gcttgttggg ctatttacat tttaagtggg caacgcgcag gagcggaaca tggccctgcg
    16921 acggtggcaa ttggttcgtt gattgcagcg ttaattttcg tgccaattgg agcgcttcag
    16981 gctggtgaag cactctggca ctggtcggtt attccattgg gtctggctgt cgctattctc
    17041 tcgaccgctc tgccttattc gctggaaatg attgccctca cccgtttgcc aacacggaca
    17101 tttggtacgc tgatgagcat ggaaccggcg ctggctgccg tttccgggat gattttcctc
    17161 ggagaaacac tgacacccat acagctactg gcgctcggcg ctatcatcgc cgcttcaatg
    17221 gggtctacgc tgacagtacg caaagagagc aaaataaaag aattagacat taattaaatt
    17281 tacatttctg catggttatg cataaccatg cagaa
//
LOCUS       DAJOSY010000091        16784 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJOSY010000091 DAJOSY010000000
VERSION     DAJOSY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.091"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(371..1567)
                     /gene="hemY"
                     /locus_tag="OHN27_003260"
     CDS             complement(371..1567)
                     /gene="hemY"
                     /locus_tag="OHN27_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCQ2287646.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRHDGLMLTL
                     QNNPPQ"
     gene            complement(1570..2781)
                     /gene="hemX"
                     /locus_tag="OHN27_003261"
     CDS             complement(1570..2781)
                     /gene="hemX"
                     /locus_tag="OHN27_003261"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCQ2287647.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(2803..3543)
                     /gene="hemD"
                     /locus_tag="OHN27_003262"
     CDS             complement(2803..3543)
                     /gene="hemD"
                     /locus_tag="OHN27_003262"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCQ2287648.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3540..4481)
                     /gene="hemC"
                     /locus_tag="OHN27_003263"
     CDS             complement(3540..4481)
                     /gene="hemC"
                     /locus_tag="OHN27_003263"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCQ2287649.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDSRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4868..7414
                     /gene="cyaA"
                     /locus_tag="OHN27_003264"
     CDS             4868..7414
                     /gene="cyaA"
                     /locus_tag="OHN27_003264"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCQ2287650.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLENWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7454..7774)
                     /gene="cyaY"
                     /locus_tag="OHN27_003265"
     CDS             complement(7454..7774)
                     /gene="cyaY"
                     /locus_tag="OHN27_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCQ2287651.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            8237..8440
                     /locus_tag="OHN27_003266"
     CDS             8237..8440
                     /locus_tag="OHN27_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2287652.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8477..9301
                     /gene="dapF"
                     /locus_tag="OHN27_003267"
     CDS             8477..9301
                     /gene="dapF"
                     /locus_tag="OHN27_003267"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCQ2287653.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9298..10005
                     /locus_tag="OHN27_003268"
     CDS             9298..10005
                     /locus_tag="OHN27_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCQ2287654.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10002..10898
                     /gene="xerC"
                     /locus_tag="OHN27_003269"
     CDS             10002..10898
                     /gene="xerC"
                     /locus_tag="OHN27_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCQ2287655.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10898..11614
                     /gene="yigB"
                     /locus_tag="OHN27_003270"
     CDS             10898..11614
                     /gene="yigB"
                     /locus_tag="OHN27_003270"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCQ2287656.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11698..13860
                     /gene="uvrD"
                     /locus_tag="OHN27_003271"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11698..13860
                     /gene="uvrD"
                     /locus_tag="OHN27_003271"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCQ2287657.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(14007..14771)
                     /locus_tag="OHN27_003272"
     CDS             complement(14007..14771)
                     /locus_tag="OHN27_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCQ2287658.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASSKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15141..16091
                     /gene="corA"
                     /locus_tag="OHN27_003273"
     CDS             15141..16091
                     /gene="corA"
                     /locus_tag="OHN27_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCQ2287659.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(16134..16514)
                     /locus_tag="OHN27_003274"
     CDS             complement(16134..16514)
                     /locus_tag="OHN27_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCQ2287660.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(16528..>16784)
                     /locus_tag="OHN27_003275"
     CDS             complement(16528..>16784)
                     /locus_tag="OHN27_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287661.1"
                     /translation="FLVIVSTIVLHYLVINMNCQRLRDSGFIYIKTYVFGTLAVYIIS
                     IITMIAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
BASE COUNT         3881 a         4349 c         4480 g         4074 t
ORIGIN      
        1 caggttaaat attcactcag gaagttatta ctcaggaagc aaagaggatt acagaattat
       61 ctcataacaa gtgttaaggg atgttatttc ccgattctct gtggcataat aaacgagtag
      121 atgctcattc catctcttat gttcgcctta gtgcctcata aactccggaa tgacgcagag
      181 ccgtttacgg tgcttatcgt ccactgacag atgtcgctta tgcctcatca gacaccatgg
      241 acacaacgtt gagtgaagca cccacttgtt gtcatacaga cctgttttaa cgcctgctcc
      301 gtaataagag caggcgtttt tttatgtgtc aggaaggccc cggaggtgct tgcccccggg
      361 tgagaaggaa ctactgtggt gggttgttct gcaacgttaa catcaaaccg tcgtgacgca
      421 tcgctgcagc ttcttccggc ttgtgcagtc tgtccagcgc gtcggcaagc catgcgtaat
      481 cgtaggcgtc cggacgttgt ttcagcgctg cgcggaaggc gagcgatgct tcctgccatt
      541 ctccgtgctt catcagtgac tggcccagtg tgctccacaa cagcgggcga tcgccgacgt
      601 ttttgatctg ctggcgcagc actttttcca gctgttccgg attgttggtt ttcagtcgag
      661 gaatcggcag cagtaggcga tcgtcgtact ggcgtttcag gccatcgatg ataatttgct
      721 gggcagtatc atgatcgtca cattcaataa gatgttccgc cattgccacc tgcaacgcta
      781 cctgatgacg cgttttccgg ctttggtttt tccaccagtt acgcaaacct tcactaccgt
      841 tatcggcacg cgcctgatcc atcaggccaa tccatgcctg ttgttccagc attgcacgat
      901 gttcttcatc accaacatgg gctttcgcca ttgatgggat gatatccagc agagaactcc
      961 atgcacctgt gcggatatac gcctgttccg ccagacgtaa tacttccgga tggcgtggcg
     1021 taacttccag cagcttatcc acgccgtggc gtgcagcatg gttttcatta cgggccagtt
     1081 gcagacgtac acgggtgatt tctaccggaa tggtgtcatt gccggccagc tccgctgcgc
     1141 gttccagatg ttggttggcg cgcgcttcat caccacgttg ttgcgcggct tcggcagcca
     1201 gtagatagtt caccaccggt tgttcagcgt gatcggcatt tttcgccatc agcttttcaa
     1261 cttgctgata atcgccttcc gccagtttca gcagcgcctg ttcggtctgc ttacgtgcac
     1321 ggcgacgctt acgcccgaca aaccacccac gggtgtgcgc gccagtgcgg aagatccgcc
     1381 gcagtagcca ctcaatggca aacagcacta ccatcgccag aatcaatatg atcgccaggc
     1441 ccgtgacgct ggtttcgata ttgtagttgt cggtctggat cagcacataa ccctgatgac
     1501 cggcaatcat cgggccaacc actatccccg caatcagcaa cacaaagagt aataacactt
     1561 ttagcatggt tattctcctt gcggcgcggc tgccggagta tcaccttgcg gtgcaggtgc
     1621 aggtgcaggt gcaggtgcag gtgcaggtgc aggtttagct tccgttgtcc ctgctgccgg
     1681 ttgtgccagc aggttacgca cgcgagtctg catcagtttt tccagcatcg cctggctttg
     1741 cagggtttcc ggaagatcca tcgagatatt ttgctggctt aactggtcca cctcgtcgag
     1801 gaacgctttg gtggtggcat catcagtatc gtagtaagca cgtacccagg tggagacgtt
     1861 ctccagcgcc tggcgataag tctcttcctg gtgacgcggt acagcttgtg ctgcgaccag
     1921 caggcgagag cgaatatttt cgcgcagata gatatcctga tttggcgcta acagcggtac
     1981 ggcggtgtca tcacgacggc gaatcgtaat gaagttgtcc ataaagttct gccagctttt
     2041 ttgcagattg atacgccatt cgctgatgga actggaaagc tcttcaccgt ctgaatccat
     2101 cggcgaacca tcgctgtcat tatcggccag acgcaggtta tctacctgat ttgaaagctg
     2161 attaagctta aggatgatgc cgtcataatc cacctgcgat actgcagaaa ggctggcgat
     2221 atcatcggta attgcccgac gaacggtaat cagactcggg tcattcatat ccgccaggct
     2281 ggcgtctgca cttttcagca acgctgcagc ggtcgtgacg tcctgatcgc tccacagctt
     2341 ccgtccggcg agtttcacca gaaaatcggc ctgagccagc agccaggttt tagcatcgct
     2401 gccggaaatg gtggcgacct tttgttggac ttcatccaac tgttttgcca gcgtttcttg
     2461 ctgacgattc gcctgcttaa gttgtgcagc ttgttgctta ataatgcctt ccagctcggc
     2521 tttttggctc tcctgggctt tttgcaatgc cgtcagttgg ttagccaggg catcgctggt
     2581 ggcggtctga ttgacggcct gttgtttacc ccagccatac aaaccgacgc ccgccgccag
     2641 agcaatagcg atagccaccg cgctgagaat caatgcggta ttgttcttac tctttttttc
     2701 tgttgcgaca ggttgtgacg tggtgtccac ggcctccctg gtctcttcaa ccacggcgga
     2761 ggttttttct tgttccgtca ttatggcttc ctgttatgag agttattgta atgcccgtaa
     2821 aagcgcatcg ttgtcagcgt tatcggcgac cttaatgtct tgccagccca gttcccgggc
     2881 gagtttcgcc aaacgctcac tgacgaccaa tagtcgacag tgtagtaacc agtgctcacg
     2941 ataccattgt gggatcagcg accagagttg ctgcaacatt tcaccgctgg taacaacgac
     3001 catcgtcacc tcgcgggatt gccagcgcat cgcttcttct gcaccatcgt aatggattgc
     3061 gcatcgttga taacattcac aaaaagtgac ctcagcaccg cgcgctgtca gggtatcccc
     3121 aattagctca cgaccgccat tgccacgtaa tatcagcgca cgtttgcccg caatattttg
     3181 taattcaggt aattgtagca agacttcgct gatttcccga tcctgcgggt agagaatctt
     3241 ctgtccgctt acggtatgta gcgccagtgc ggtggtgcgt ccaatggcga aataatcagg
     3301 tagtcggggc catttacgat cttgctgatg cagctgtgat tgggcaaaag caaccgcgtg
     3361 ttgcgagagg gcaaacaaca gatcgctctc ccccagcgcc gccagttgat cagcaagttg
     3421 cggtaattgt tgacccggag aaaactcaat cagcggaaaa tgccaggcca cctgccccag
     3481 tgtgcgcaga cggctcacta actcttctcc agcgggagac gggcgggtga ccaggatact
     3541 catgccgggg cgtctccgtt atagacttca gcgaggatct cgcgcgcgcc gttattcagt
     3601 agctcttctg ccagcgaaat ccccatttgt tcggcatctt gcggcgcacc gcggcgttca
     3661 ccgcgaataa tctgcgaacc gtccggcgcg ccgaccagcg cacgcagcca gatttcgcca
     3721 tcaataagct cggcgtagct accaattggc acctgacatc cgccttcgag acgggtattc
     3781 atggcgcgtt ctgcggtaac gcgcagtgca gtttcgtggt gattcagcgc ggcaagcagc
     3841 tcgcgagtgc gtgaatcatc aaggcggcat tcaataccca ccgcaccttg tcctaccgcc
     3901 ggaagagaaa tctcgggtgg caacgcggcg cgaatacgtg actccagacc taaacgtttt
     3961 agtccggcta cggcaagaat gatggcatcg tattcgccgt tatccagttt gctcaggcga
     4021 gtgccgacgt tgccgcgcag ggagcggata atcagatccg gacggcgttc agccagttgg
     4081 cactggcgac gtaaactgga cgtcccgacg atactgcctg ccggtaacgc atccagactg
     4141 tcatagttat tggacacaaa ggcatcgcga ggatcttcac gctcacaaat agtgaccagt
     4201 cccagacctt gcgggaattc aaccggcaca tctttcattg agtgtacggc gatatcggcg
     4261 cgattttcga ggagcgcgac ttccagctct tttacaaata agccttttcc gcctactttc
     4321 gccagcggcg tatcaagaat cacatcgccg cgcgtcacca tcggtaccag ttcaacgacc
     4381 aggcccggat ggctcgccat caacttgtct ttgacatagt gtgcctgcca gagtgcaagt
     4441 gggctttggc gtgtggcaat tcttaaaaca ttgtctaaca tgcttgttac cgtcattatc
     4501 atccgtggtc catcctaaca tccttgccag agtgatgtca gtgttgtggt gaaacgtaga
     4561 cgcctgcgca aaccgtaaaa tgaggtctgg cagtggatcc tgacaggcgt ttcacgccgt
     4621 tgtaataagg aatttacaga gaataaacgg tgctacactt gtatgtagcg catctttctt
     4681 tacggtcaat cagcaaggtg ttaaattgat cacgttttag accatttttt cgtcgtgaaa
     4741 ctaaaaaaac caggcgcgaa aagtggtaac ggttaccttt gacatacgaa atatcccgaa
     4801 tgccgcgtgt taccgttgat gttggcggaa tcacagtcat gacgggtagc aaatcaggcg
     4861 atacgtcttg tacctctata ttgagactct gaaacagaga ctggatgcca taaatcaatt
     4921 gcgtgtggat cgcgcgcttg ctgctatggg gcctgcattc caacaggtct acagtctact
     4981 gccgacattg ttgcactatc accatccgct aatgccgggt taccttgatg gtaacgttcc
     5041 caaaggcatt tgcctttaca cgcctgatga aactcaacgc cactacctga acgagcttga
     5101 actgtatcgt ggaatgtcag tacaggatcc gccgaaaggt gagcttccaa ttactggtgt
     5161 atacaccatg ggcagcacct cgtccgtagg gcaaagttgt tcctctgacc tggatatctg
     5221 ggtctgtcat caatcctggc tcgatagcga agagcgccaa ttgctacaac gtaaatgtag
     5281 cctgctggaa aactgggccg cctcgctggg tgtggaagtc agcttcttcc tgattgatga
     5341 aaaccgcttc cgtcataatg aaagcggcag cctggggggc gaagattgtg gctccaccca
     5401 gcatatactg ctgcttgacg aattttatcg taccgccgtg cgtctcgccg gtaagcgtat
     5461 tctgtggaat atggtgccgt gcgacgaaga agagcattac gacgactatg tgatgacgct
     5521 ttacgcgcag ggcgtgctga cgccaaatga atggctggat ctcggtggct taagctcgct
     5581 ttctgctgaa gagtactttg gtgccagcct ttggcagctc tacaagagta tcgattcccc
     5641 atacaaagcg gtactgaaaa cactgctgct ggaagcctat tcctgggaat acccgaaccc
     5701 acgtctgctg gcgaaagata tcaaacagcg tttgcacgac ggcgagattg tatcgtttgg
     5761 tctcgatcca tactgcatga tgctggagcg tgttactgaa tacctgacgg cgattgaaga
     5821 ttttacccgt ctggatttag tacgtcgctg cttctattta aaagtgtgcg aaaagctcag
     5881 ccgtgaacgc gcctgcgtag gctggcgtcg cgcagtgttg agccagttag tgagcgagtg
     5941 gggttgggac gaagctcgtc tggcaatgct cgataaccgc gctaactgga agattgatca
     6001 ggtgcgtgag gcgcacaacg agttgctcga cgcgatgatg cagagctacc gtaatctgat
     6061 ccgctttgcg cgtcgcaata accttagcgt ctccgccagt ccgcaggata tcggcgtgct
     6121 gacgcgtaag ctgtatgccg cgtttgaagc attaccaggt aaagtgacgc tggtaaaccc
     6181 gcagatttca cccgatctct cggaaccgaa tctgaccttt atttatgtgc cgccgggccg
     6241 ggctaaccgt tcaggttggt atctgtataa ccgcgcgcca aatattgagt cgatcatcag
     6301 ccatcagccg ctggaatata accgttacct gaataaactg gtggcgtggg catggtttaa
     6361 cggcctgctg acctcgcgca cccgtttgta tattaaaggt aacggcattg tcgatttgcc
     6421 taagttgcag gagatggtcg ccgacgtgtc gcaccatttc ccgctgcgct tacctgcacc
     6481 gacaccgaag gcgctctaca gcccgtgtga gatccgccat ctggcgatta tcgttaacct
     6541 ggaatatgac ccgacagcgg cgttccgcaa tcaggtggtg catttcgatt tccgtaagct
     6601 ggatgtcttc agctttggcg agaatcaaaa ttgcctggta ggtagcgttg acctgctgta
     6661 ccgcaactcg tggaacgaag tgcgtacgct gcacttcaac ggcgagcaat cgatgatcga
     6721 agccctgaaa actattctcg gcaaaatgca tcaggacgcc gcaccgccag atagcgtgga
     6781 agtcttctgt tatagccagc atctgcgcgg cttaattcgt actcgcgtgc agcaactggt
     6841 ttctgagtgt attgaattgc gtctttccag cacccgccag gaaaccgggc gtttcaaggc
     6901 gctgcgcgtt tctggtcaaa cctgggggtt gttcttcgaa cgcctgaatg tatcggtaca
     6961 gaaactggaa aacgccatcg agttttatgg cgcgatttcg cataacaaac tgcacggcct
     7021 gtcagtgcag gttgaaacca atcacgtcaa attaccggcg gtggtggacg gctttgccag
     7081 cgaagggatc atccagttct ttttcgaaga aacgcaagac gagaatggct ttaatatcta
     7141 cattctcgac gaaagcaacc gggttgaggt atatcaccac tgcgaaggca gcaaagagga
     7201 gctggtacgt gacgtcagtc gcttctactc gtcatcgcat gaccgtttta cctacggctc
     7261 aagcttcatc aacttcaacc tgccgcagtt ctatcagatt gtgaaggttg atggtcgtga
     7321 acaggtgatt ccgttccgca caaaatctat cggtaacatg ccgcctgcca atcaggatca
     7381 cgatacgccg ctattacagc aatatttttc gtgatgaacg tgccggaaag cgaggcttat
     7441 ccggcatgca atcttagcgg aaactgactg tttcacccgc ctgctgcgtc gccgcctgtt
     7501 ccagcaaatc ccagaaggtt tcgccgctgc gatcacaaat ccactcatcg cctttcaggt
     7561 caaaatggta gccgccctgt ttggttgcca gccatacctg gtgcagcggc tcctggcggt
     7621 tgataatgat tttgctgcca ttctcaaagg taatggtcag tacgccgccg ttgatttcgc
     7681 agtcgatatc gctgtcgcca tcccagtcgt ccaggcgttc ttcaatggtc agccagagtt
     7741 gatcagccag gcgatgaaat tcactgtcgt tcattgttgt atcctgtttt taagtgatgg
     7801 cggcagtata gcggcatggg gtcagggctt caaagtttgc acctctgcgg ctgcgttccg
     7861 gcacgattca tccgtcaccg gaataatgat gtctctgtgt agcgaaagat ttgtctcttc
     7921 attagggcgc agttacacca cgtctttccc tgtttctggt aaacattatg atcaggttta
     7981 ccgagcgagc atcctcacgc tgacggaact aaaaaagaca acaaacaaac cacattgcga
     8041 tagtgcataa agccatcctg gcgcgaggtg ccgatcacga aactaccagc aaaacataaa
     8101 tccccacgag taagcgttat actcgcagca tttcctcact tttcagactt cataaagagt
     8161 cgctaaacgc ttgcttttac gtcttctcct gcgatgatag aaagcagaaa gcgatgaact
     8221 ttacaggcaa tccataatga aaaacgtgtt taaggcactc actgtattac ttactctctt
     8281 cagcctgacg ggctgcggtc tgaaaggtcc gctctatttc ccgcctgcag ataaaaacgc
     8341 accgccgccg accaaaccgg tagagacgca aacgcaatcc acggtgccgg ataaaaacga
     8401 tcgcgccact ggcgatggtc catcccaggt gaattactaa aagtcagttt ctgtacccgc
     8461 gtgattggag taaatgatgc agttctcgaa aatgcatggc cttggcaacg attttatggt
     8521 cgtcgacgcg gtaacgcaga atgtcttttt ttcaccggag ctgattcgtc gcctggctga
     8581 tcggcacctg ggggtagggt ttgaccaact gctggtggtt gagccgccgt atgatcctga
     8641 actggatttt cactatcgca ttttcaatgc tgatggcagt gaagtggcgc agtgcggcaa
     8701 cggtgcgcgc tgctttgccc gttttgtgcg tctgaaagga ctgaccaata agcgtgatat
     8761 ccgcgtcagc accgccaacg ggcggatggt tctgaccgtc accgatgatg atctggtccg
     8821 cgtaaatatg ggcgaaccca acttcgaacc ttccgccgtg ccgtttcgcg ctaacaaagc
     8881 ggaaaagacc tatattatgc gcgccgccga gcagacaatc ttatgcggcg tggtgtcgat
     8941 gggaaatccg cattgcgtga ttcaggtcga tgatgtcgat accgcggcgg tagaaacgct
     9001 tggtcctgtt ctggaaagcc acgagcgttt tccggagcgc gccaatatcg gttttatgca
     9061 agtggttaag cgcgagcata ttcgtttacg cgtttatgag cgtggggcag gagaaaccca
     9121 ggcctgcggc agcggcgcgt gtgcggcggt tgcagtaggg attcagcaag gtttgctggc
     9181 cgaagaagta cgcgtggaac tccccggcgg tcgtcttgat atcgcctgga aaggtccggg
     9241 tcacccgtta tatatgactg gcccggcggt acatgtctac gacggattta ttcatctatg
     9301 aagcaaccag gggaagaact gcaggaaaca ctcacggagc ttgatgaccg ggcggttgtc
     9361 gattatctga ttaaaaatcc tgagtttttt atccgtaatg cgcgcgcagt agaagcgata
     9421 cgtgtgccgc atccggtacg cggcaccgtt tcgttggtcg agtggcacat ggcccgcgca
     9481 cgtaatcata ttcatgttct ggaagagaac atggcgctgt tgatggaaca ggctatcgcc
     9541 aacgaaggcc tgttttatcg cctactctac ctgcagcgca gtctcaccgc cgccagcagt
     9601 ctcgacgata tgctgatgcg ctttcaccgc tgggcgcgcg atctcggcct ggcaggtgcg
     9661 agtctgcgcc tgtttccgga tcgctggcgc ttaggtgcgc cgtcgaacca cactcatctg
     9721 gcattaagcc gtcagtcttt cgaaccgctg cgtattcagc gtttggggca ggaacagcac
     9781 tatcttgggc cgcttaacgg accagagctg ctggtggtgc taccggaagc gaaagcggtg
     9841 ggatcggtgg cgatgtcgat gctgggaagc gatgctgatt tgggtgtcgt gctgtttacc
     9901 agtcgcgatg ccagtcacta tcaacaaggg caaggaacgc agttacttca tgaaattgcg
     9961 ctgatgttgc cggagcttct ggagcgttgg attgaacgcg tatgaccgat ttacacaccg
    10021 atgtagaacg ctacctacgt tatctgagcg tggagcgcca gcttagcccg ataaccctgc
    10081 ttaactacca gcgtcagctt gaggcgatca tcaattttgc cagcgaaaac ggcctgcaaa
    10141 gctggcagca atgtgatgtg acgatggtgc gcaattttgc tgtacgcagt cgccgtaaag
    10201 ggctgggagc agcaagtctg gcgttacggc tttctgcgct acgtagcttt tttgactggc
    10261 tggtcagcca gaacgaactc aaagctaacc cggcgaaagg tgtttcggca ccgaaagcgc
    10321 cgcgtcatct gccgaaaaac atcgacgtcg acgatatgaa tcggctgctg gatattgata
    10381 tcaatgatcc cctcgctgta cgcgaccgtg caatgctgga agtgatgtac ggcgcgggtc
    10441 tgcgtctttc tgagctggtg gggctggata ttaaacacct cgacctggag tctggtgaag
    10501 tgtgggttat ggggaaaggc agcaaagagc gccgcctgcc gattggtcgc aacgctgtgg
    10561 cgtggattga gcactggctt gatttgcgcg acctgtttgg tagcgaagac gacgcgcttt
    10621 ttctgtcgaa actgggcaag cgtatctccg cgcgtaatgt gcagaaacgc tttgccgaat
    10681 ggggcataaa acaagggctg aataatcacg ttcatccgca taaattacgt cactcgttcg
    10741 ccacgcatat gctggagtcg agcggcgatc ttcgtggtgt gcaggagctg ctgggtcatg
    10801 ccaacctctc caccacgcaa atctatactc atcttgattt tcaacacctt gcctcggtgt
    10861 acgatgcggc gcatccacgc gccaaacggg ggaaataatg cgtttttacc ggcctttggg
    10921 gcgcatctcg gcgctcacct ttgacctgga tgataccctt tacgataacc gtccggtgat
    10981 tttgcgcacc gagcgagagg cgcttacctt tgtgcaaaat tatcatccgg cgctgcgcag
    11041 cttccagaat gaagatctgc aacgcctgcg ccaggcggta cgggaagcgg aacccgagat
    11101 ttatcacgac gtgacgcgct ggcgttttcg ttcgattgaa caagcgatgc tcgacgccgg
    11161 gctgagtgcc gaagaagcca gtgcaggcgc acacgcagca atgatcaact ttgccaaatg
    11221 gcgcagccga atcgacgtcc cgcagcaaac tcacgacacc ttaaaacagc tggcgaagaa
    11281 atggccgctg gtggcgatca ccaacggtaa cgcccagccg gagctgtttg gtttggggga
    11341 ttattttgag tttgtgctgc gcgctggccc gcacgggcgc tcaaaaccgt tcagcgatat
    11401 gtactttttg gctgcggaaa aactcaacgt gccgatcggc gagatcttac atgttgggga
    11461 cgatctcacc actgacgtgg gtggggcaat tcgcagcgga atgcaggctt gttggatcag
    11521 accggaaaat ggcgatctga tgcaaacctg ggacagccgt ttactgccgc atctggaaat
    11581 ttcccggttg gcatctctga cctcgctgat ataatcagca aatctgtata tatacccagc
    11641 tttttggcgg agggcgttgc gcttctccgc ccaacctatt tttacgcggc ggtgccaatg
    11701 gacgtttctt acctgctcga cagccttaat gacaaacagc gcgaagcggt ggccgcgcca
    11761 cgcagcaacc ttctggtgct ggcgggcgcg ggcagtggta agacgcgcgt actggtgcat
    11821 cgtatcgcct ggttgatgag cgtggaaaac tgctcgccat actcgattat ggcggtgacg
    11881 tttaccaaca aagcggcggc ggagatgcgt catcgtatcg ggcaactgat gggcacgagc
    11941 cagggcggta tgtgggtcgg caccttccac gggctggcgc accgtttgct gcgtgcgcac
    12001 catatggacg ccaatctgcc gcaggatttc cagatcctcg acagtgaaga ccagctacgc
    12061 ctgcttaagc gtctgatcaa agccatgaac ctcgacgaga agcagtggcc gccgcggcag
    12121 gcaatgtggt acatcaacag ccagaaagat gaaggcctgc gtccgcatca tattcaaagc
    12181 tacggtaatc cggtggagca gacctggcag aaggtgtatc aggcgtatca ggaagcgtgt
    12241 gaccgcgcgg gcctggtgga cttcgccgag ctgctgctgc gcgctcacga gttgtggctt
    12301 aacaagccgc atatcctgca acactaccgc gaacgtttta ccaatatcct ggtggacgaa
    12361 ttccaggata ccaacaacat tcagtacgcg tggatccgcc tgctggcggg cgacaccggc
    12421 aaagtgatga tcgtcggtga tgacgaccag tcaatctacg gctggcgcgg ggcgcaggtg
    12481 gagaatattc agcgtttcct taatgatttc cccggtgccg aaactattcg tctggagcaa
    12541 aactaccgct ctaccagcaa tattctgagc gccgctaacg ccctgattga aaacaataac
    12601 gggcgtctgg gtaaaaaact gtggaccgat ggcgcggacg gtgagcctat ttccctctat
    12661 tgcgctttta acgaactcga tgaagcgcgt tttgtggtta accgcatcaa aacctggcag
    12721 gacaacggcg gggcgcttgc cgagtgcgcc attctctacc gcagtaacgc ccagtcgcgt
    12781 gtgctggaag aagcactgtt gcaggcgagt atgccgtacc gtatttacgg cgggatgcgc
    12841 ttcttcgaac gccaggaaat caaagatgcg ctctcgtatc tgcgcctgat tgccaaccgt
    12901 aacgacgacg cggcctttga gcgtgtggtg aatacgccaa cgcggggtat tggtgaccgg
    12961 acgctggacg tggtacgtca gacatcgcgc gatcgccagt taacactctg gcaggcatgt
    13021 cgtgaactgt tgcaggaaaa agccctcgcc ggacgtgctg ccagcgcctt acagcggttt
    13081 atggaactga tcgacgcctt agcgcaggaa actgccgata tgccgctgca tgtacagact
    13141 gaccgggtaa ttaaagactc cggcctgcgt accatgtacg agcaggagaa gggcgaaaaa
    13201 ggtcagacgc gtatcgaaaa cttagaggaa ctggtgacgg caacgcgcca gttcagctac
    13261 aacgaagaag acgaagattt aatgccgctg caggcgttcc tatcccatgc ggcgctggaa
    13321 gcaggcgaag ggcaggcgga tacctggcag gatgcggtac aactgatgac catgcactcg
    13381 gcgaaaggtc tggagttccc gcaggtgttt atcgtcggta tggaagaggg catgttccca
    13441 agccagatgt cgctggatga aggcgggcgt ctggaagaag aacgccgtct ggcctacgtt
    13501 ggcgtaaccc gcgcgatgca gaaactgacg ctgacctacg cggaaactcg ccgtctgtat
    13561 ggcaaagagg tttaccatcg cccgtcgcgc tttattggcg agctgccgga agagtgtgtg
    13621 gaagaggtgc gcctgcgcgc cacggtaagc cgcccggtca gccatcagcg gatgggtacg
    13681 ccgatggtcg agaacgacag cggctacaag ctcggccagc gtgtacgcca cgctaagttt
    13741 ggtgaaggca ccatcgtcaa tatggaaggc agcggtgaac atagccgttt gcaggtggca
    13801 ttccagggcc agggaatcaa atggctggtg gcagcttacg cccggctgga gtcggtgtaa
    13861 cgttgccgga tgcggtgctg cgcaccttat ttggcctaaa aaatcattca gattcaataa
    13921 attgcaacgt catgtaggcc ggatagggcg tttacgccgc atccggcatc tgcgccatct
    13981 tcagtatctg acacaaaact atcgttttaa cctttccgct cgacggaaat catggtgaca
    14041 aaaggatagc gttgccacgg gattgccccg cctttcatat acatatgtga aatcgtgcca
    14101 tcaagataaa gcagctgctc aacgttcagt ttcgctttgg cataacaggc aaaatcataa
    14161 aaatttgttg cctgctggct caacaaaaac acggcgttcc catgtttatt aatcccaaca
    14221 ccgttacgaa ttttgcttga ggcgacgttg ggatgaatac gcggattaat tacaccgttt
    14281 tccatcaaca ttggccctga ctgcaccgca aactgaatct ctttactggt tttgaaggca
    14341 tccagacgaa cgatgccgac tttatctccc gcgacataaa acacgccgcc aggacggata
    14401 aagaaattcc cttcacctga agcgagattt aacgccacct tctgctgacc gttttcgatg
    14461 tacaaaccga gcggcgcata gctttcatca tagatgccgc cgttcatcgc catctgcacc
    14521 tgaccctgac tattcatatc cgccagcaga gcatgtaacg ttccccacgc ttcgccattg
    14581 gctttttgcc agtacatttt cacccgctct gtttgaggat taacggtata cgcctgtacg
    14641 gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa cagcaaacaa gggaagtaag
    14701 gtgagggcga gaaaaatccg tttgagattc aaggtgatca ttcctttacc aatgagtagc
    14761 cgatgcgcca ttataggtcc tggatgtggg atttttttat cctgttagcg accttgacga
    14821 gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt tctgttgcgt gttgacggca
    14881 aaattttgct ggcgtaacat gcgcgcacga tcactctaag aggacattcg ccttggacac
    14941 acccagtaga tactggctca ctatcctgtc atccaggatc aactcctaag gctatccctt
    15001 tttgctgata gccttagcgg ttgtcagcga cctcaatttt tcccgtcgcg ctgagtcagg
    15061 ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc accgaactgt ccgatatttt
    15121 aagcattggg agtcccggtc atgctgagcg catttcaact ggaaaataac cgactgaccc
    15181 ggctggaagt cgaagagtca caaccccttg taaatgcagt atggattgat cttgtcgaac
    15241 cggacgacga cgagcgactg cgcgtacaat ctgaacttgg tcagagcctg gcaacccgcc
    15301 cggaactgga agacatcgaa gcatcggcac gtttctttga agacgacgac ggcctgcata
    15361 ttcactcctt cttcttcttt gaagatgcgg aagatcacgc cggtaactcc actgtggcat
    15421 ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg tgaactgccc gcttttcgtc
    15481 tgtatcgtat gcgtgcccgt agccagtcga tggtggacgg taacgcctac gagctgctgc
    15541 tggatctgtt cgaaaccaaa atcgaacagt tggcagatga aattgaaaat atctatagcg
    15601 acctggagca gttgagccgg gtgattatgg aagggcatca gggcgatgag tacgacgagg
    15661 cgctctccac tctggcggaa ctggaagata tcggctggaa agtgcgcctg tgtctgatgg
    15721 atacccagcg tgcgctcaac ttcctggtgc gtaaagcgcg tttaccgggt gggcaactgg
    15781 agcaggcgcg tgaaatcctg cgagatatcg aatccctgct gccgcataac gaatccctgt
    15841 tccagaaggt gaacttcctg atgcaggcgg caatgggttt tatcaacatc gagcagaacc
    15901 gcatcatcaa aattttctcg gtggtatccg tcgtgttcct gccgccaacg ctggttgcct
    15961 ccagctacgg gatgaacttt gagtttatgc cagaactgaa gtggagtttc ggctacccag
    16021 gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta tctgtacttt aagcggaaga
    16081 actggttgta aaaaacgaga gcggtggctt agtctggcta agccacctgt tattcaaagg
    16141 ctccaggtat ttaacccttt tacctctttc tcatagaacc atttgttcgt gttaacagca
    16201 acataggctg ctacggcaat tcccagaatg ttaacgccaa ttagtgcacc aacaaataga
    16261 gatagaatac caagtaatag aactataatt gcttttttcc ataaccccag gacaaataaa
    16321 tatatccagg aacagaagaa agcaatgaaa ttcatttgaa tagttaagcg ttgtcttact
    16381 tttaatgctt taaatgctgc tttatattct ggtgttgccc cccagaatcc aggaaaacca
    16441 tgttgatcat aaaaattaaa tcggtatttc catttttcac ttaatgaacc atcgttcata
    16501 tattccttac tcataaatac tccataacta ttgttttgat gaatcagtag gtgcaagcat
    16561 tagcatactg aaagtggaaa aataacaaat caaaaaaatc atcgaaccat tgcctgaaca
    16621 ggcaaaatct tcggctatca ttgtgatgat agagatgata tatactgcta atgtaccaaa
    16681 aacataagtt tttatataga tgaaaccact atcacggagt cgctggcaat tcatgttgat
    16741 gacgagataa tggagtacga tggtagagac tataacaaga aagc
//
LOCUS       DAJOSY010000092        16530 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJOSY010000092 DAJOSY010000000
VERSION     DAJOSY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.092"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            45..164
                     /gene="hemP"
                     /locus_tag="OHN27_003276"
     CDS             45..164
                     /gene="hemP"
                     /locus_tag="OHN27_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCQ2287662.1"
                     /translation="MRRISSQTLLGPDGKLIIDHDGQEYLLRKTQAGKLLLTK"
     gene            complement(168..1604)
                     /locus_tag="OHN27_003277"
     CDS             complement(168..1604)
                     /locus_tag="OHN27_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCQ2287663.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGERMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTGQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1667..2380)
                     /gene="ydiV"
                     /locus_tag="OHN27_003278"
     CDS             complement(1667..2380)
                     /gene="ydiV"
                     /locus_tag="OHN27_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCQ2287664.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(2627..3091)
                     /locus_tag="OHN27_003279"
     CDS             complement(2627..3091)
                     /locus_tag="OHN27_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCQ2287665.1"
                     /translation="MRFCLILITALFLVGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(3169..3918)
                     /gene="btuD"
                     /locus_tag="OHN27_003280"
     CDS             complement(3169..3918)
                     /gene="btuD"
                     /locus_tag="OHN27_003280"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCQ2287666.1"
                     /translation="MSIVMQLQDVAESTRLGPFSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(3918..4469)
                     /gene="btuE"
                     /locus_tag="OHN27_003281"
     CDS             complement(3918..4469)
                     /gene="btuE"
                     /locus_tag="OHN27_003281"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCQ2287667.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKFAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(4532..5512)
                     /gene="btuC"
                     /locus_tag="OHN27_003282"
     CDS             complement(4532..5512)
                     /gene="btuC"
                     /locus_tag="OHN27_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCQ2287668.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSSSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(5613..5912)
                     /gene="ihfA"
                     /locus_tag="OHN27_003283"
     CDS             complement(5613..5912)
                     /gene="ihfA"
                     /locus_tag="OHN27_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCQ2287669.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(5917..8304)
                     /gene="pheT"
                     /locus_tag="OHN27_003284"
     CDS             complement(5917..8304)
                     /gene="pheT"
                     /locus_tag="OHN27_003284"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCQ2287670.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(8319..9302)
                     /gene="pheS"
                     /locus_tag="OHN27_003285"
     CDS             complement(8319..9302)
                     /gene="pheS"
                     /locus_tag="OHN27_003285"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCQ2287671.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(9511..9635)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(9585..9629)
                     /gene="pheM"
                     /locus_tag="OHN27_003286"
     CDS             complement(9585..9629)
                     /gene="pheM"
                     /locus_tag="OHN27_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCQ2287672.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(9752..10108)
                     /gene="rplT"
                     /locus_tag="OHN27_003287"
     CDS             complement(9752..10108)
                     /gene="rplT"
                     /locus_tag="OHN27_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCQ2287673.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(10161..10358)
                     /gene="rpmI"
                     /locus_tag="OHN27_003288"
     CDS             complement(10161..10358)
                     /gene="rpmI"
                     /locus_tag="OHN27_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCQ2287674.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(10455..10997)
                     /gene="infC"
                     /locus_tag="OHN27_003289"
     CDS             complement(10455..10997)
                     /gene="infC"
                     /locus_tag="OHN27_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCQ2287675.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(11001..12929)
                     /gene="thrS"
                     /locus_tag="OHN27_003290"
     CDS             complement(11001..12929)
                     /gene="thrS"
                     /locus_tag="OHN27_003290"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCQ2287676.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            13453..15352
                     /gene="espL1"
                     /locus_tag="OHN27_003291"
                     /pseudo
     CDS             13453..15352
                     /gene="espL1"
                     /locus_tag="OHN27_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            15524..15631
                     /locus_tag="OHN27_003292"
     CDS             15524..15631
                     /locus_tag="OHN27_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287677.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(15684..16442)
                     /locus_tag="OHN27_003293"
     CDS             complement(15684..16442)
                     /locus_tag="OHN27_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCQ2287678.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGHTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT         4267 a         4318 c         3753 g         4192 t
ORIGIN      
        1 cgcctgaaac ggatgccaat cacaagaccg cttccccgca gcctattcgg cgaatttcca
       61 gccagacact gttaggtccg gatggcaaac tgattatcga tcatgacggg caagaatatc
      121 tgctccgtaa aactcaggct ggcaagctgc tgttgaccaa gtagccttta actcgagcag
      181 ctgacttcca gccgtttacc ccagtcaggt ggacgactga catagtcatc atctctgtcg
      241 ctgaaaggat ttcgcaacgc ctcatgcagg cggtgcaatt ccgtcatatc acccttttct
      301 gccgcttcaa tcgcccgttg cgccaaccag ttgcgcaata ccagagcggg gttaacgctt
      361 tgcatcagtt gctgacgctc actatcgcta acctcgtctt gttgcaaacg cccccgataa
      421 cgggcaaacc agtcatcaaa tgccgcacga tcaataaact catcacgtag cggtgacgcc
      481 gcgctgtgct gcccggtcag actcagcatg cggaatgtgc gggtataatc gctgcgctct
      541 cgcgccatca gactgaataa ttcattcagt agcgcgttat cctctttttg ctccgtcatg
      601 aagcccagtt tctgccgcat ccgttctcca taatgcgtca acaaaacctg ctgatagctg
      661 tccagggcct cattcagggc atctacggca acaaatggcg acaatgtctg cgccagacgc
      721 tgtaaattcc acaacgcgac ggcaggttga ttatcaaagc tgtaacgccc ttgatgatcc
      781 gagtgattac aaataaaacc gggttcgtaa tcatcaagaa aaccaaacgg cccgtaatca
      841 agcgtcagcc ccagcagcga catgttgtcg gtattcatca ccccatgagc aaagccgacc
      901 gtctgccatt gggcaattaa cgacgcggta cgtgcgacaa catcgctaaa ccagagacgg
      961 tatttgtcct catcatcttc aagatgtgac cagtaatgac gaatagcaaa gtcagctaac
     1021 tgacgaacct tttccgactc gcggcggtag taaaaatgtt cgaaatgacc aaagcgcaga
     1081 tgacttggtg ccacacgcat cagcatcgcg cctggctccg ccgtttcccg atacactggc
     1141 gaatcgctgg tgacgatact taacgcgcgg gtcgtcggaa tgcccagata atgcatcgcc
     1201 tcactggcga gactttctcg tatcgtcgaa cgtaaaaccg cccgtccatc acccattcgc
     1261 gaataaggcg tcaggccagc acctttcaga tgccagtcca ttgtagtgcc atcagcaagc
     1321 agttgttcgc cgagtaaaat gccgcgccca tcacccagtt ggcccgccca gacgccgaac
     1381 tgatgaccac tgtaaacctg cgccagtggt gacataccag gcagtagggc ttcgccgccc
     1441 cagacacctg cgccattttt aaacagcgac gatggaatac tcagcgtgtt agccagttcg
     1501 gtattatgcc aaatcagccg ggcattattt aaaggcgtag gggaaagtgc tgtataggtt
     1561 tctggcaatt catcgcgcca gcgggtaaca aaagacaggg tcatagatcc tcctgtttga
     1621 tagtgtagac ggttactctc gtcttaaaca ccagcaaaca gaagggttat cgctgaacca
     1681 acgtcgttat ctgatttatg ggaacggcag gccacaggca tccctggaga gcatggaaat
     1741 cgaagggggt aatctgtgcc aatatttctg ccgtatcaat gcctcccgca atgatgcaat
     1801 tgcaacaagg ggaaatttgc gcctggatcg cacgaataaa cggttcgaag gagcggtgcg
     1861 tgatttgttg ctgaataaaa cttttatcca gcatgacgcg ggtgaaaaga ccatcaaaaa
     1921 ccgctttcat tgtgctattg cctgctccca aattacctaa cacaagggga tacacttgcg
     1981 ataaggataa aagaccgcga ttatcttttc cttcattgag atgcgggtaa ttctcattaa
     2041 tcaacagttc aataaacgga tattttaata gttcgcctgc gtaattatcc ctctctaata
     2101 ataatgtagc cacttgcggc gttaaattaa gccaggcaaa gagtttatgt tgaataaaaa
     2161 aatgttggca tgattttagt aattccaact gctctgaaaa taattgccaa tgttgctcct
     2221 cggtaagttg cgctataacc cgactcgtcg ggatacgcac cgtgccatct tcactggaga
     2281 aatgggtaat taactcaacg ccgacaagga cttgctggtt atctcttatc ggcaggaaat
     2341 agcaatcaga atgataaaga ttctccaaaa aaatcttcat ggtaaccgtc cctctcgaag
     2401 gatgtttttc agtatccggc taaaagggat aaggccataa gacataacaa ttacagaagg
     2461 agtaactttc atttgttcca tgttaaccac tttttcaggg gtcctttttt tagattatcc
     2521 tgattataaa cgaataatcc atttacggaa tttttgtctg caaaatacta ctgtattcag
     2581 gagtaaatgg acggcgatgt atcatcgccg tccatttttc gacatactag attcgtctcg
     2641 cctgccagaa atttttctgc caatagacat tatcaagtga ggaacgcatc actcccttgc
     2701 tggtagaggc gtggataaat tggttgttgg tatcgtaaat acctacatgc aaaccatttt
     2761 gtccggaccc cgttttgaaa aagaccaggt caccaggcag caactcgtct ttatcaattt
     2821 gcgtgccgat agaggcttgt tctttggttt ctcggggcag ctgcaaatcg aaacgatcgc
     2881 gcatcgttac aaccacaaat cccgaacagt ccacaccgcg ccgcgtcatg ccaccataac
     2941 gatacggcgt gccatgccag ctttgtagct ggtcgttcaa accggcaata acggtaatcg
     3001 aatcagaaag tctggcattt ggcggcggtg ctttatggtg gctacacccg accagaaaca
     3061 gtgctgtgat caaaataagg cagaaacgca ttccgtacgg ttcctctgtt ttttattctt
     3121 gcattaattt agcgtcgtaa ttacccgatt ttcaagatac taatgaaatc agatggtcga
     3181 aatcagcatt ctgtgacctt cgatatccag acggcgaaaa ttcatcccat aggcctgcgc
     3241 cagatttggc ggcgtgagca cctcttccct gcgtccactg gccagcattt ttccaccttt
     3301 tagcaaccac gcccgatgcg catgacgcaa tgtgtggttg agatcgtgac tgctcatcac
     3361 aatcgccagt ccttgctgac acagcgcgct cagaattttg tctaacgcac tttgttgcgc
     3421 aacatcaaga ctgttcatcg gctcatcaag aagcagcaat tggcctgcgg gattggcttg
     3481 tggtgtgatt tgcagcacca ccgcagcaag acgtacgcgt tgccattcac cgccggaaag
     3541 ttgattggtg ctacgtccga gtttgtcatc aagagccagc gcccctgcga catcattcag
     3601 tagttcggta cgcgttttat cgtgctgatg cagtgtcagg tagtgccaga ccggcattgc
     3661 aaacggcggc gtctgctgtt gtgaaaggta ggcgcgatgc agcgcgagtt ttgttgcgga
     3721 ccatgcttcc agtggttgcc ccgcgaactg aatgcttccc ttaccgctgg tcattccggc
     3781 cattcgtgca agcaaggtac tcttacccgc gccattcggc cccaccaggt gcaggatctc
     3841 cccagcccga acctcgccag aaaacggccc caggcgggta gattccgcaa catcctgtaa
     3901 ctgcatcaca atagacatta ttttgccaac gccagtttaa tgctttccat cacaatggga
     3961 tcttccggcg tcatatccgg ggaaaaacgc tggatgacca atccgtccct gccaactaaa
     4021 aatttctcaa aattccataa aatatcatca gggtacagcg gtgcacgacc tttgctgacc
     4081 atacgggcat agaatccgct ctcttccggc gcgactgcgg tcggcgctgc ggcaatcaat
     4141 ttttgataca gcggatggcg tccttcgcca ttaacttcaa tcttactgaa catcgggaac
     4201 gtcacccccc atgtggtggt acagtaagtt ttaatctctt catcgctgcc cggttcttgt
     4261 tccagaaact ggttgcacgg gaatcccagc accacaaaac ctcgatcggc ccaggctttc
     4321 tgaatattct ccaactgctc atattgcggc gttaagccac actttgaggc gacattgaca
     4381 atcaacagca cattaccggc gaacttctcc agcgtggtca cttcaccgtc gatatctttc
     4441 actacggtcg tcagaatgga atcttgcatc gtttctcctg ggtgtggtca gtaaaaatct
     4501 tagcttttaa tcatagaccg tctttttgcg gctaacgtcc tgcttttaac aataaccaga
     4561 taaacaccgg cgcacctaac gttgcggtga ccacgccaat aggcagctct gcggcagcta
     4621 atgccaggcg cgctacaata tcggccagca gcaatgcgct cgcccctgcc agcgcgcagc
     4681 cgggaagtaa tacgcgatga tcggttaaac cacacagccg gagaatatgt gggatcacca
     4741 gaccaataaa gccgatagca cccgccagcg ccacactgac gccaaccatc cagccggtcg
     4801 ctgccaccag cacattgcgc cagaaccaca ggggtaaacc cagttgccgc gccgagatct
     4861 cgccaagtgc taacatattc atcggcctgg actgacaaca gatccacaac aacacgggga
     4921 tcaatgccag catcagccag ctttgccgcc agtctacgcc gccaaaaccg cccatcatcc
     4981 agtacatcag ctgacgcaaa tcaacagagg tggaaaagta gatagcccac gtcattagtg
     5041 cgctacagat aatccctaat gcaacgccag ccagcaataa ccgactgctc gaaagatgac
     5101 gacgggcgaa acgtaagagt attaaagtga tgataagcgc gccagcaatc gcacacagcc
     5161 ctagcgccca gttggggagt tgcccttgcc caagcaatac cgcggcgata agccccacgc
     5221 ctgcgccgtt agagacgcca agtagtccag gttctgccag aggattttca aacaacgcct
     5281 gcattacagc gccggatata gccagcgccg caccaaccag caatacagcc agcgtacgtg
     5341 gcaggcgaat ttgccagacg aacagttcgc cacgaggagt aaaccagtca cctggcgaga
     5401 tccattgttc accggcgcaa aggcttaaga gaagcgccag cagcatcaaa actgacaggc
     5461 ataataacca gcgaatattt tgtcgctgtt gttggcgggc aagtgtcagc atggtatccg
     5521 ttctgctgaa gtgtcatggc gttgatttta cggtgactct tcgacagtga aaagaaaaaa
     5581 ggccgcagag cggccttttt agttagatca gattactcgt ctttgggcga agcgttttcg
     5641 acccggcttt ttaacttctg cccgggtctg aaggtcacca cgcgccgtgc tgtaatggga
     5701 atatcctcgc ccgttttcgg gttacgtccc gggcgttgat tcttatcacg cagatcgaag
     5761 ttaccaaaac cagagagttt cacctgttcg ccgttttcca gagcgcgacg gatctcttcg
     5821 aaaaacagtt caaccagttc tttggcatcc cgcttgctaa gcccaagctt atcaaacaga
     5881 tattctgaca tttcagcttt tgtaagcgcc ataggttcaa tccctcaatg atgcctggaa
     5941 tcgctctttt aatgcctcta cacatttggc gacggtagcg gcaatctcct cttcttcgag
     6001 tgtacggctg gtatcttgca ggatcaggct tatggcgagg ctcttatacc cctccgcaac
     6061 acccttaccg cggtacacgt caaataagtt tacgccaact acctgattta cgccaacttt
     6121 cttacattcg gataaaatat ccgctgcggg aacgttttct gcgaccacca ccgcgatgtc
     6181 acgacggttc gccgggaagc gagaaatctc gcgcgcctga ggcaccacgc ggtctgcgag
     6241 cttgttccac tccagttcga acaccagagt gcgaccgtta agatccagtt tacgttccag
     6301 ttcaggatga acaactccaa caaaaccaat acgttcacct ttcagataaa tcgctgcgga
     6361 ttgccccgga tgcagtgccg gattcgcttc tgcacggaac tcaacctcat tcagtttacc
     6421 ggtcaggtcg agaacggatt caagatcgcc tttcaaatca tagaaatcaa cggtctcttt
     6481 tgccaggttc cagtgctctt cgtaacggtt accgcaaatc acaccggcta acatcagatc
     6541 ctgacgaatg cccaacggtg cctgagtatc tggtacgaaa cgcagaccgc tttcgaaaat
     6601 gcgcacacgg ttctgctgac ggttctggtt gtacaccacg gttgccagca ggccggtcca
     6661 cagagaaaga cgcattgctg acatttcaac agagatcggg cttggcagca gtaaggcttc
     6721 aacgcctgga tggatcatct gctgcacttt cggatcaacg aagctgtagg tgatcacttc
     6781 ctgatagcct ttgtcgttga gcagcgtttt cacgcgcttg agcgacaggt cagcttcacg
     6841 gtgagtaccc ataatcaggc ttgcctgtac cggctcatcc gggatgttgt tgtagccgta
     6901 aacacgcgcg acttcttcaa ccagatcttc ttcaatctcc atatcgaaac gccagctcgg
     6961 cgcaactgcc tgccattcgt ctttgccttc ggtcacttcg cagccgagac gacgcagaat
     7021 gtcagttacc tgctcatccg caatatgatg gccgatcagg cgatccagtt tgctacgacg
     7081 tagagtgatg gttgcacgct tcggcagcgt tgcttcgttg gtgatatcaa ttaccgggcc
     7141 agcctcacca ccgcagatgt cgatcagcag acgggtcgca cgttccatcg ctttatgctg
     7201 cagtgccgga tcaacgccac gctcataacg gtgagacgca tcggtatgca ggccatgacg
     7261 acgagcacga ccggtgatag acagcgggct aaagaacgcg cattccagca gcacgttttg
     7321 tgtttcgtca ttcacgccag agtgttcgcc accgaagatg ccgcccatcg ccagcgcctt
     7381 gttgtggtcg gcgataacca gggtgtcagc attcagcttc gcttcagtgc catcaagcag
     7441 caccagcgtt tcaccctctt tcgccatccg caccacaatg ccgccttcaa tgcgatcttt
     7501 atcgaaagcg tgcatcggct ggcccagttc gagcagcaca tagttggtga cgtcaacaac
     7561 tgcatcgata gaacggatcc cgcaacgacg cagtttttct ttcatccaca gcggagttgg
     7621 cgctttaacg ttaatgcctt ttaccacacg gccaagataa cgcgggcagg cttccggcgc
     7681 ttcgactgta atcggcagcg tgtcgtcgat ggtcgcacca accggaacga tttccggttc
     7741 aaccagcggc agctgattca gcacggcaac gtcacgcgca acaccaatga tacctaaaca
     7801 gtcggcacgg tttggcgtca cgctgatttc gatggtgttg tcatcaagtt tcaggtattc
     7861 acggatgtcg gtgccaatcg gcgcatccgc aggcagctcg ataatgccgc tatgatcgtc
     7921 agaaattccc agctcagaaa aggagcacag catcccttca gacggttcac cacgcagttt
     7981 cgccgcttta attttgaaat cacccggcag aacagcacca atggtagcta ccgccacacg
     8041 caggccctga cggcagtttg gcgcaccgca gacgatgtcc agcaggcgat cgccgccaac
     8101 attcactttt gtcacacgca gtttgtcagc gttcggatgc tgcgcacact caaccacttc
     8161 accaacgacc acgccgtgga agctaccggc aaccggttct acaccgtcaa cttccaggcc
     8221 cgccatagtg atttgattcg ccagcgtatc gctatcaatc gccgggttca cccattcgcg
     8281 taaccacagt tcactgaatt tcataatcta ttcctgcctt atttaaactg tttgaggaaa
     8341 cgcagatcgt tttcgaagaa tgaacgcagg tcggtgacgc cgtaacgcaa catagtcaga
     8401 cgctccatcc ccatcccgaa ggcgaaacca gagtaaactt ccgggtcgat gccaacgtta
     8461 cgcagcacgt tcggatgcac catcccgcag cccagcactt ccagccattt accgttttta
     8521 cccatgacgt caacttctgc agaaggctcg gtaaacggga agtaggaagg acggaagcga
     8581 atctgcaaat cttcctcaaa gaagttacgc aggaagtcgt gcagcgtgcc tttcaggttg
     8641 gtaaagctga tgttggtatc aacaatcaga ccttccatct gatggaacat cggcgtgtga
     8701 gtctggtcgt agtcgttacg ataaacacgg ccaggcgcga tgatacgaat cggtggctgc
     8761 tgggctttca tggtgcggat ctgtacgcca gaggtctggg tacgcagcag gcgggtagcg
     8821 tcaaaccaga aagtgtcgtg gtcagcgcgc gccgggtggt gaccaggaat gttcagagca
     8881 tcgaagttat gataatcgtc ttcgatttcc ggcccggttg ccacggtaaa gccaagctca
     8941 ccgaagaaac tttcgatacg gtcgatggta cgggtaaccg gatgcagacc gccgttttca
     9001 atgcgacgac ctggcagaga gacatcaatc gtttccgccg ccagacgcgc attcagtgca
     9061 gcgctttcca gttccgcttt acgcgcattc agcgcctgct gaacctgctc tttcgcttcg
     9121 ttgataaccg caccagctgc cggacgctct tctggcggca gctcacgcag ggtcgtcatc
     9181 tgaagggtta agtgcccttt tttacccaaa tattcgacgc gcacattatc taacgcggca
     9241 acatctgacg cctggctaat ggccgccttc gcactggcaa ccagttctgc gagatgtgac
     9301 atggttttcc tcattgtgtc agtggtgaca ctggttcgtt ggacttagag cctatcccat
     9361 caggctattt tacttgccat tttggtcccg ggcagtgctc gaaatcctca cgtacttaag
     9421 tacgctccgg tttctccgca ctggccgtgt ccagactggc tgcgacaatt acgcctgata
     9481 gactggcttt ttatttttca aaacgcgcat acaaaaaaag cctccactgg gaggctttca
     9541 ggcgctgttt tccgtttctc ttctcacgcg ctagcctcct ggattcaggt gctaaagtaa
     9601 aaaaagaagc ggaaaatagc agcattcatt gcttgcgtta ccttttggta ctcttcaaaa
     9661 gacctttatt gaaaaggcta cggcgataaa agtcaatgtt ttgatggcgt tgaaacgaaa
     9721 agagggagac tagctccctc tttcaactgg cttatgccag agctgctttc gctttttcaa
     9781 ccagagcggt gaacgctact ttgtcgaata ctgcgatatc agccaggatc ttacggtcga
     9841 tttcaacaga ggcttttttc aggccattga tgaatttgct gtaagaaata ccgttctgac
     9901 gtgctgctgc gttgatacgc gcaatccaca gttgacggaa ctgacgctta cgttgacgac
     9961 ggtcacggta agcatactga ccagctttga taacagcctg gaaggcaacg cggtatacgc
    10021 gagaacgcgc accgtagtag cctttagctt gtttcaaaat tttcttgtga cgtgcacgtg
    10081 caataacacc acgttttacg cgagccatat gtgctctcct gtatctatat tctaattaaa
    10141 aagttaaaaa cgttaacggc ttatgcgtac ggcaggcacg cgattaccag gcccagatcg
    10201 cctttggaaa ccatggcttt cggacgcagg tgacgtttac gtttggtcgc ttttttggtc
    10261 agaatgtgac gcaggttagc gtgcttgtgc ttaaaaccac ctttaccggt ttttttgaag
    10321 cgcttagcag caccgcgtac ggtcttaatt tttggcattt taataacttc cacttcgcat
    10381 tgttaataaa cgaaacaaag gcgaacaaag cctgtgaagc ccgaaggctc cacagacagt
    10441 gctacttgaa ggccttactg tttcttctta ggagcgagca ccatgatcat ctggcggcct
    10501 tcgatcttcg ttgggaagga ttcgaccact gccagttctt gcaaatcgtc tttcacgcga
    10561 ttaagcactt ccataccgat ttgctggtgc gccatctcac gaccgcggaa acgcagcgtg
    10621 attttggctt tatcaccctc ttcgagaaag cgaatcaggc tgcggagttt tacctgatag
    10681 tcgccttcat ctgtaccagg acggaattta atttccttaa cctggataac tttttgcttt
    10741 ttcttctgtt ccttagaaga cttgctcttt tcatagagga atttgccgta atccattata
    10801 cgacaaaccg gcggctcggc gttagggctg atctcgacta agtctactcc ggcttcttct
    10861 gctttctcca gagcttctct cagactcaca ataccaagct gctcgccttc cagacctgtt
    10921 aagcgaactt cctgggcgcg aatttcgcca ttgatacggt tagggcgcgc cgtttgaact
    10981 cgttttccgc ctttaatacc ttattcctcc aattgtttaa gactgcggct gcgaatctct
    11041 tgttgcagct tctcgatcac ttcatttacg tccatgcttc ccaggtcttt accacggcgg
    11101 gtgcgaacgg caactttgcc tgattccacc tctttatcac cacagaccag catatatggg
    11161 acgcgacgca aagtgtgctc gcggatttta aagccaatct tctcatttct caagtctgct
    11221 ttaacacgaa tgcccgcatt tgatagtttt tgcgtcaatt cgttaacgta ttcagactgt
    11281 gaatcggtaa tattcatgat aacaacctga accggcgcaa gccaggtcgg gaagaaacca
    11341 gcgaactctt cggtcaggat accgatgaaa cgttccatcg accccagaat tgcgcggtga
    11401 atcattaccg gtactttacg ttcattgtct tcgcctacat aagaagcgct cagacgagac
    11461 ggcaaagaga agtccagctg tactgtaccg cactgccatg cacgatcgag gcagtcatac
    11521 agggtaaatt caattttcgg accgtagaaa gcgccttcac ccagttgata ttcaaacggg
    11581 atgttgtttt cttccagcgc aaccgccagg tccgcctcag cacgatccca catttcgtcg
    11641 ctgccaatac gtttttcagg acgagtggag agtttgacga cgatcttctc gaagccaaaa
    11701 gtgctgtaca tatcatagac taaacggata catccgttaa cttcatcgcg aatttgttct
    11761 tcagtacaga agatatgcgc gtcatcctgg gtaaatccac gcacgcgcat caggccatgc
    11821 agcgaacctg acggctcgtt acggtggcag ctaccaaact cggccatacg cagcggcaga
    11881 tcgcgataag acttcagccc ctggttgaaa atttgtacgt gacccgggca gttcatcggc
    11941 ttaatgcagt attcacggtt ctcagaagat gtggtgaaca ttgcatcttt gtagttgtcc
    12001 cagtgaccgg ttttttccca caggacacgg tccatcatga acggaccttt aacttcctga
    12061 tactggtact ctttcagttt agaacgaaca aacacttcca gttcacggaa gatggtccag
    12121 ccgtcgttgt gccagaatac catacccggc gcttcttcct gcatatggta caggtcgagc
    12181 tgtttaccga ttttacggtg gtcgcgtttc gcggcttctt ccaggcgctg caggtaagcg
    12241 ttaagtgctt ttttgtctgc ccacgccgta ccgtaaatac gttgcaacat tttgttgttg
    12301 ctgtcgccac gccagtaagc ccctgccgtt ttcattagtt tgaaatgatg gcagaaacgc
    12361 atgttcggta cgtgcggacc gcggcacata tcgacatatt cttcatggaa gtacagacct
    12421 ggcttgtcat catgggcgat gttttcgtca agaatggaga ctttgtagct ctccccacgg
    12481 ttggcgaaag tttcacgcgc ttcgtgccag ctgactttct tcttaatgac gtcgtagttt
    12541 ttctcagcaa gctcatgcat ccgcttctcg agtgcttcga catcttcctg ggttaacgtg
    12601 cggtcaagat caacgtcgta ataaaaaccg ttgtcaataa ccgggccgat tgccattttg
    12661 gtatgcggcc aaagttgttt aatcgcgtgc cctaacaggt gcgcacagga gtgacgaatg
    12721 atctccagac cttcttcgtc tttggcggta atgatcgaca gttgtgcgtc gttttcaatc
    12781 agatcgcaag catcaaccag ttcgccatta acgcgccctg cgatacaggc tttcgccaga
    12841 cctggaccaa tgtccagcgc aacatccatg gggcttacag cgtgatcgta atggcgttgg
    12901 ctgccatcag gaagagttat aacaggcatt ttatatcctt atttgcagtg gtgacccaca
    12961 cgaaagatca catacaaaga aaaatttgtt tattaacagt tcattgcgaa accatctagc
    13021 caacaaatgc taaattggtt cgcaatcagg tacacaaatg aaagacatta acctctcacc
    13081 ttccacccgg ttgagcgatg ttaacactaa aaaaagggag attgtacctt tccgtttcac
    13141 attgattcga ttcgcaattc gtttgtagtg agtacaccag caaaacgctt aaagaacaac
    13201 tgataacatc acggtgcgtc tgaaaggttt actgcgagcg gatttacagc agcataaact
    13261 gaatccagca aagttcgttg cgtgatttgg cggtagtatt ttaaacgctc atgagattaa
    13321 tgacgaagtg gtcatatcac aatgataaaa gtgacacaat tcttataaca atttttcgtg
    13381 cacatttcgt tctggcgata ataattaatc atcatcctca aaacctccag atatatataa
    13441 ggcgaacaga ttatgagtca aaacgatatc attatcagaa ctcattataa gtctcctcat
    13501 agattgcaca tcgatagcga cataccaaca ccttcatcag agcctattaa tcaatttgcg
    13561 cgccagctca tcaccctact tgatacctct gacttaagtt cgatgctgtc atactgtgtt
    13621 actcaggaat ttaccgcaaa ctgtcgaaaa atatcacaaa attgttattc cactgccctt
    13681 tttaccatta actttgccac ttcacccatc catacagaaa atatactcat tacattacac
    13741 tataaaaaag aaatcatttc cttattactg gaaaccacgc ctattaaagc taaccatttg
    13801 cgaagcatac tggattatat tgaacaggaa cagttaactg ctgaagatcg taaccattgt
    13861 atgaaactgt ctaaaaaaat ccatagagaa aaaaactata cacccaacag taaatctcaa
    13921 tggtagtgca tgttttttgc aatctccttc tgacgctatt ttttgtcgcc atctgtcatt
    13981 gcaatacgcc cttgattcat tgagaaatgg aaaaggcaaa gtcaacctga ttaaacatta
    14041 ctcctccgtt gaatccatac agcagcatgt ccccttagtc cgggacgcgg agttcagagc
    14101 attacttcgc catcctcctg cagggagtcg cgttatcgcg agtaaggatt ttggcttcgc
    14161 tttagatatt ttcttttgtc gaatgatggc aaacaatgtc agtcatatgt ccgcgatttt
    14221 atatatagac aatcatactt tgtcagtaag gctacgaata aagcagtcag tgtatggaca
    14281 attaaattat gttgtgtccg tttacgaccc gaacgatacc aacgttgccg tcagagacac
    14341 ccacaggaca gcacggggct ttctttcgct tgataagttc atcagttcag gtcccgatgc
    14401 tcagacctgg gctgatagat atgttcgcaa ctgtgcaatt gctattctgc ccctattacc
    14461 tgtgggagtt ccaggggcta ttttcgcggg tattgcatca cgaatgccat ttgcccctat
    14521 acatccatcg gcaatgttgt taataatggc tacaggccag actcaacagc ttattacatt
    14581 attcaaacag ttgcccatac tccctgaaaa agaaatcatt gaaataataa ctgcgcagaa
    14641 tagcgttggt acacctgctt tatttttggc tatgatgaac ggacatactg acaacgtgaa
    14701 aatatttatg caagaaattc agtcactggt agataatcac atcattcatg aagataatct
    14761 ggttaaatta ctgcaaacta aaagtgctaa cgaaacacct ggactttata tctccatgtt
    14821 gtatggattc gatgaaataa tcgatatctt tctgaatgca ttaaccactc caatagcaca
    14881 agagctttta aacaaaaaac tggtgatgag tattttagcc atgaaaatac atgatggtga
    14941 gccaggatta tacgccgcaa tggaaaataa tcaccctttg tgtgtcacac ggttcctctc
    15001 taaaattaat ggcatcgcct ttaaatacaa gttgagcaaa gctaacatca tggatttatt
    15061 aaaaggagct acagcacagg gaacccctgc attatacatc gccatgagca agggtaatga
    15121 agacgtcgtg ttatcttata tatcgacgct gggtgctttt gcaaaaaaac attcttttag
    15181 tcaacatcag ttatttacac tattggccgc taaaaatcat gacaacatgt cagctgttca
    15241 tatagccatt catcataagc attataaaac tgtagaaaca tattatgctg ctattaatgc
    15301 aatcagccaa agcctgagtt ttagtgctga tgaaataaag acgtatttat aaccagctat
    15361 ttacgatata tatcgataca taattttatt tttttcataa aacgcagaaa cagtgaccat
    15421 gtgcacaacg ccacccgacg gctgtaataa caaaaaatcc cttgatgcct gtcccttttg
    15481 ttacactccg ttatcacgca caagagatat gcaggacact ggtatgccga ctaaacgctt
    15541 tgataaaaaa cactggaaga tggtggtggt gctactggca atctgtggcg ctatgttgtt
    15601 gctacgttgg gcagcaatga tttggggctg agcagtgaca aacgaagaca gccagaccgc
    15661 ataacacggt ccggcagata attttacatg ctgtagccca gcgataaggt tgtccgacgg
    15721 tcggtatgtt ccggtgcaga ctctggaggt tcagagttcc atgtgacgtt gtaagccacc
    15781 ttcagtccaa aatgttcatt aatagcaaca tttaatgcgc tttcggagtt cagcgttgtg
    15841 tcttccgcgc caaagaccga aacaccctgc gtaaatttag cgttgtcagt caactgccat
    15901 gcataagccc cggaggcata gcccagcggc tgggtttcac tggcattgtc ggtatatttg
    15961 tcgtaacgca cacctggacc gaattcaaag cggaaactgt gtaccgggcc attcaaaaac
    16021 tgacgaccat aacccgcggt caacacatcg cgctctcgat agccgttata acggtctgtc
    16081 agccagcttg cctgaccaaa taaatagtca taatcagtta aattaaaacg gctacgcccg
    16141 cccgccgcat atttttctga agaacgctca tcattagaag aagtattact ggcgttcccc
    16201 cacagcgacc aggcagtggt gtgtccatac caggtcatgg tggtgtcagc cgtaagtgag
    16261 gagcttttcg tattgcctga ttgtgcaaga tatcctgcgt tcagattacc ttcgaaaggt
    16321 tttttagcgc tggcaggatc atccatgaca gtaaaaacgg aatcatcggc agctgcattc
    16381 agtgacgcaa acatgccccc cgccaacata acgatggcag gaactgtctt caaaagcttc
    16441 atttatcaag agtccgtaca acaaaaaaag agaccatcgc ggtcccggaa actttcttaa
    16501 ggatcaaaga ttagcgtccc tggaaaggta
//
LOCUS       DAJOSY010000093        16416 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJOSY010000093 DAJOSY010000000
VERSION     DAJOSY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.093"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(290..1939)
                     /gene="pgi"
                     /locus_tag="OHN27_003294"
     CDS             complement(290..1939)
                     /gene="pgi"
                     /locus_tag="OHN27_003294"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCQ2287679.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2464..3813
                     /gene="lysC"
                     /locus_tag="OHN27_003295"
     CDS             2464..3813
                     /gene="lysC"
                     /locus_tag="OHN27_003295"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCQ2287680.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDSRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4066..4338
                     /locus_tag="OHN27_003296"
     CDS             4066..4338
                     /locus_tag="OHN27_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCQ2287681.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     DYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4471..5343)
                     /gene="rluF"
                     /locus_tag="OHN27_003297"
     CDS             complement(4471..5343)
                     /gene="rluF"
                     /locus_tag="OHN27_003297"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCQ2287682.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5555..6244
                     /gene="pepE"
                     /locus_tag="OHN27_003298"
     CDS             5555..6244
                     /gene="pepE"
                     /locus_tag="OHN27_003298"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCQ2287683.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6335..7966)
                     /locus_tag="OHN27_003299"
     CDS             complement(6335..7966)
                     /locus_tag="OHN27_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCQ2287684.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(8186..11869)
                     /gene="metH"
                     /locus_tag="OHN27_003300"
     CDS             complement(8186..11869)
                     /gene="metH"
                     /locus_tag="OHN27_003300"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCQ2287685.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            12069..12893
                     /gene="iclR"
                     /locus_tag="OHN27_003301"
     CDS             12069..12893
                     /gene="iclR"
                     /locus_tag="OHN27_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCQ2287686.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            13210..14784
                     /locus_tag="OHN27_003302"
     CDS             13210..14784
                     /locus_tag="OHN27_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCQ2287687.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPI
                     FEEYLMEILPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAA
                     QYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALVSSRPEGRCVAACGDFGL
                     VMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKI
                     RIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDE
                     ITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSGHMNVINTIFNA
                     LPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSNHARLFGF
                     TAEDIMDFWQHKAPQKYSAFELAFELGHRVIAELILNTLNKMAESFGFTDNPRYIAEK
                     NYMEALLKKASPHTVR"
     gene            complement(14753..>16416)
                     /gene="aceK"
                     /locus_tag="OHN27_003303"
     CDS             complement(14753..>16416)
                     /gene="aceK"
                     /locus_tag="OHN27_003303"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCQ2287688.1"
                     /translation="EVTSGAQQRFEQADWHAVQQAMKNRIHLYDHHVGLVVEQLRCIT
                     NGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQ
                     PERRFRTIPRPLAKDFHPDHGWESLLMRVISDLPLRLRWQNKSRDIHYIIRHLTETLG
                     TDNLAESHLQVANELFYRNKAAWLVGKLITSSGTLPFLLPIHQTDDGELFIDTCLTTT
                     AEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREY
                     LVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQL
                     VKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEKITDLGEQIVIRHLYIER
                     RMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYD
                     YDEICYMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFE
                     EMHADLFRADYWRALQNRIREGHVEDVYAYRRRQRFSVRYGEMLF"
BASE COUNT         4009 a         4270 c         4102 g         4035 t
ORIGIN      
        1 gaaataaaca acgattaaaa atctatttta ggattaacct cacggtatga tttccgttaa
       61 atcacagaca aggggattta tctgataaaa aaatgccgac ataacgtcgg cattgttatt
      121 aaggttattt aatcaatcgt gctatgtatt aattgccgaa tacgatgtac atatcggcat
      181 cgacctgtag gcctgataag acgcgaccgc gtcgcatcag gcatcggttg ccggatgcgg
      241 cgtgaacgcc ttatccggcc tacatagcgg catcaggcat gaacgatgat taaccgcgcc
      301 acgctttata gcggttaatc agaccattgg tcgagctatc gtggctgctg atttctttat
      361 catctttcag ctctggcaga atacggtttg ccagttgttt acccagctcc acgccccact
      421 ggtcgaaggt gaagatgttc aggatcacgc cctgagtaaa gattttgtgc tcatacagcg
      481 caatcaacgc ccccaggctg aacggagtga tttcgcgcag caggatggag ttggtcgggc
      541 ggttaccttc gaacactttg aacggcacca cgtagtcaag cgttgccgga tctttaccct
      601 gatcgcgata ttcctgctca accacttcac gggatttacc aaacgccagc gcttcggtct
      661 gggcgaagaa gttagacagc agtttctggt ggtgatcaga gagcgggtta tgggtgatag
      721 ccggggcgat gaaatcgcac ggtaccattt tggttccctg gtggatcagc tggtagaacg
      781 cgtgctgacc gttagtgcct ggttcacccc agataatcgg gccagtctgg taatccacaa
      841 cgttaccgtt acggtcaaca tacttaccgt tggactccat attgccctgc tggaagtacg
      901 ccgcgaaacg gtgcatatac tggtcatacg gcagaatcgc ttcagtttcc gcaccaaaga
      961 aattgttgta ccagatgcca atcagcgcca gcagtacagg caggtttttc tcggcaggcg
     1021 tggtggagaa atgcttgtcc atcgcgtgtg cgccagaaag cagttcaacg aagttatcaa
     1081 agccgatgga gagaacaatc gacaggccaa tcgctgacca caaagagtaa cggccgccaa
     1141 cccagtccca gaactcgaac atgttggcag tatcaatacc aaactcgcca acggctttgg
     1201 cattggtgga gagcgcagcg aagtgtttcg caacgtgttt ttcatcacct gccgctttca
     1261 ggaaccagtc acgcgcgcta tgggcgttgg tcatggtttc ctgagtggtg aaggttttag
     1321 atgctaccag gaacagcgtg gtttccgggt ttactttttt cagcacttca gcgatgtgag
     1381 tcccatcgac gttagaaaca aagtgcatgt tcaggtggtt tttgtacgga cgcagtgctt
     1441 cggtcaccat gtatgggccg aggtcagaac cgccgatccc gatgttcact acgtcagtga
     1501 ttgctttgcc ggtataacct ttccactcac cggaaataat cgcttctgag aaggttttca
     1561 tcttctccag caccgcgttg acttccggca ttacgtcttt gccatcaacc aaaatcgggg
     1621 tattgctacg gttacgcagc gctacgtgca gcacggcgcg gttttcagtg cggttgatct
     1681 tctcgccaga gaacatcgac ttaatcgcgc ccgccagatc gcactctttc gccagatcct
     1741 gtaatttcgc cagcgtctct tcggtgatgc ggtttttgga gtaatccacc agcatctgat
     1801 cgtcgaatgt tgcggagaac ttagaaaaac gatcgccgtc tttagcaaaa agatcggcga
     1861 tcgtaacgtc tttcatttca tcgaagtgtt tctgtagtgc ctgccaggca gcggtctgcg
     1921 ttggattgat gtttttcatt agcaatactc ttctgatttt gagaattgtg actttggaag
     1981 attgtagcgc cagtcacaga aaaatgtgat ggttttagtg ccgttagcgt aatgttgagt
     2041 gtaaaccctt agcgcagtga agcatttatt agctgaacta ctgaccgcca ggagtggatg
     2101 aaaaatccgc atgaccccgt cgttgacaac cgccccgctc accctttatt tataaatgta
     2161 ctacctgcgc tagcgcaggc cagaagaggc gcgttgccca agtaacggtg ttggaggagc
     2221 cagtcctgtg ataacacctg agggggtgca tcgccgaggt gattgaacgg ctggccacgt
     2281 tcatcatcgg ctacaggggc tgaatcccct gggttgtcac cagaagcgtt cgcagtcggg
     2341 cgtttcgcaa gtggtggagc acttctgggt gaaaatagta gcgaagtatc gctctgcgcc
     2401 cacccgtctt ccgctcttcc cttgtgccaa ggctgaaaat ggatcccctg acacgaggta
     2461 gttatgtctg aaattgttgt ctccaaattt ggcggtacca gcgtagctga ttttgacgcc
     2521 atgaaccgca gcgctgatat tgtgctttct gatgccaacg tgcgtttagt agtcctctcg
     2581 gcttctgctg gtatcactaa tctgctggtc gctttagcgg aagggctgga acctggcgag
     2641 cgattcgaaa aactcgacgc tattcgcaac atccagtttg ccattctgga acgtctgcgt
     2701 tacccgaacg ttatccgtga agagattgaa cgtctgctgg agaacattac tgttctggca
     2761 gaagcggcgg cgctggcaac gtctccggcg ctgacagatg agctggtcag ccacggcgag
     2821 ctgatgtcga ccctgctgtt tgttgagatc ctgcgcgaac gcgatgttca ggcacagtgg
     2881 tttgatgtac gtaaagtgat gcgtaccaac gaccgatttg gtcgtgcaga gccagatgta
     2941 gccgcgctgg cggaactggc cgcgctgcag ctgctcccac gcctcaatga cggcttagtg
     3001 atcacccagg gatttatcgg tagcgaaaat aaaggtcgta caacgacgct tggccgtgga
     3061 ggcagcgatt atacggcagc cttgctggcg gaggctttac acgcatctcg tgttgatatc
     3121 tggaccgacg tcccgggcat ctacaccacc gatccacgcg tggtttccgc agcaaaacgc
     3181 attgatgaaa tcgcgtttgc cgaagcggca gagatggcaa cttttggtgc aaaagtactg
     3241 catccagcaa cgttgctccc cgcagtacgc agcgatatcc cggtctttgt cggctccagc
     3301 aaagactcac gcgcaggtgg tacgctggtg tgcaataaaa ctgaaaatcc gccgctgttc
     3361 cgcgcgctgg cgcttcgtcg caatcagact ctactcactt tgcacagcct gaatatgctg
     3421 cattctcgcg gtttcctcgc ggaagttttc ggcatcctcg cgcggcataa tatttcggta
     3481 gacttaatca ccacgtcaga agtgagcgtg gcattaaccc ttgataccac cggttcaacc
     3541 tccactggcg atacgttgct gacgcaatct ctgctgatgg agctttccgc actgtgtcgg
     3601 gtggaggtgg aagaaggtct ggcgctggtc gcgttgattg gcaatgacct gtcaaaagcc
     3661 tgcggcgttg gcaaagaggt attcggcgta ctggaaccgt tcaacattcg catgatttgt
     3721 tatggcgcat ccagccataa cctgtgcttc ctggtgcccg gcgaagatgc cgagcaggtg
     3781 gtgcaaaaac tgcatagtaa tttgtttgag taaatactgt atggcctgga agctatattt
     3841 cgggccgtat tgattttctt gtcactatgc tcatcaataa acgagcctgt actctgttaa
     3901 ccagcgtctt tatcggagaa taattgcctt taattttttt atctgcatct ctaattaatt
     3961 atcgaaagag ataaatagtt aagagaaggc aaaatgaata ttatcagttc tgctcgcaaa
     4021 cgaattcccg tacactgaac tctctggctc actgagggca ccatcatggc actcccccgt
     4081 attacccaaa aagagatgac tgaacgcgag cagcgtgaac ttaaaacact gctggatcgg
     4141 gcgcggattg cgcatggtcg cgtattgacc aactccgaaa ccaacagcat taagaaagat
     4201 tacatcgata aattaatggt tgagcgtgag gctgaagcga aaaaagcccg ccagttgaag
     4261 aaaaagcagg cttataaacc ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg
     4321 acgcgcggca ggcgctaatc cacaatcaac gcctgagatt ttcactcgtc agtgtgaaca
     4381 caattttccc ctcaccctaa cccttttccc gaaggggcga ggggactgtc cgggcacttt
     4441 tgggccgttt tcatcagtct gaggcgttaa tcagcgcccc ttctttttac gccccggcga
     4501 ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt tccgccgttt tttccatctt
     4561 aacgactgga cgtttgatgc ccgctgtttt cggtttggcc ttcactttag gttttgcctc
     4621 ggaagaggaa ttttcgatga gcttaaagag gtcgattaac tcatcatcag ttaaatcgcg
     4681 ccactcaccc agcggaatgc cgcttaaact gacgttcata atgcgcgtac gttccagctt
     4741 tttcacttcg taaccaaagt gctcgcacat gcgacggatc tgacggttca gcccctgcac
     4801 cagggtaatg cggaagacaa acggcgcttc ttttttgact ttacactttt tagtcactgt
     4861 cccgaggatt ggcacccccg cccccatacc acgaataaat tcatcggtaa tcggtttatc
     4921 gaccgtaacc agatactctt tctcatgatc gttacccgca cggaggatct tattcaccag
     4981 atcgccgtga ttggtgagga aaatcagccc ctgggagtct ttatccaggc ggccaatcgg
     5041 gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta tcgcgctcgc catcttcggt
     5101 ggtgctgaca ataccgacgg gcttgttcag ggcgataagt accaaatctt cggcttcccg
     5161 aggctcaatc aactgaccgt ttactttcac aacgtcgccg ggtttcacct gatcgccaat
     5221 ggtggctcgc ttgccattaa ggaacacatt gccttgctcg atatagcgat ccgcttcgcg
     5281 gcgtgagcaa attccgcttt cgctgatgta tttatttaaa cggactgatg agtcgggcag
     5341 cataaattct cctgtaaaaa acggaatata ccgtaccttt gggttgataa aaaatagtca
     5401 tgagcgaact atttgttatt tccattggct tgaattcgtt acctgctcaa atcactttcc
     5461 agaagcaatg tgcgacatcg tgagccatgt cagtaagtgg ttaaattcgc agtggtagca
     5521 tgcctcccgt gatttcttac taaccggaga agtaatggaa ctgcttttat tgagtaactc
     5581 gacgctgccg ggtaaagcct ggctggaaca tgcgctgccg ctgattgctg aacagttgca
     5641 gggtcgccgt tcagcggtat ttattccttt cgctggcgta acgcagacct gggatgatta
     5701 cacagcgaaa acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag
     5761 cgttgtcgat cccgttgccg ctattgaaaa tgctgagatc gtgattgtcg gcggcggtaa
     5821 tactttccag ttgctgaaac agtgccgcga acgcgggctg ctggcaccaa ttactgacgt
     5881 ggtgaaacgt ggcgcgctgt atattggctg gagcgcaggc gctaaccttg cttgcccaac
     5941 tattcgtacc accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct
     6001 gttcccgctg caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga
     6061 aacccgtgag cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg
     6121 tctaccggaa ggtaactgga tcacagtgag taaaggtcac gctacgctgg gtggcccgaa
     6181 caccacttat gtgtttaagg ctggtgaaga agcggttccg ctggaagctg gtcaccgttt
     6241 ttaagtgaat tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgacgctt
     6301 gacgcgtctt atcaggccta caggattcac aatattaata ctcgtcccgt ccttcatctt
     6361 catccggctg ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc aggcgctgca
     6421 tatctcccag taagcctaaa tgtagcgaac tggtttcaat gctctgcacg ttttgctgat
     6481 gcaggcgatc gacgtgggcg tgggaatagc ggcgattaag aatgcgaaaa cgatgtttgc
     6541 tgcgacgcaa acgacgagcg ctggtgacat cgccagagaa gaacaccgac attgccagtt
     6601 ttaaattact gagcaattgc tcatagagcg catccagttc cttcaagcca tcaagcgaaa
     6661 atgcccgccg tgctgccagc gatttatcag caatttcgct gcccatgcgc tcgacgatat
     6721 cggaagcctg ttcaaggttg agcgacattt caataatctc cgcccagcga cgcgactctt
     6781 cttctgccag ttcctctttt ggcatccgcg ccagatacag cttaatggcg gtatagagaa
     6841 cgttgatatc atctgccagc ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca
     6901 ctttattcag cccttccatc atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg
     6961 cggcgttcgc cagagcaagc gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc
     7021 gtagctgggt atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg
     7081 ggtcaacaaa tggcagcatg accaggcaac gtacaaggtt gtagaagacg tggaaataga
     7141 tcaccagttc cgcttttggc aatgacaact tccccattgt ctctgccagc aaatggacaa
     7201 acggcaggat aatcaggcta cccaccagct taaacagcaa actacccagc gcgacacggc
     7261 gggctgcggc attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggttag
     7321 caccaatcac cagacagagc gccacgggga aggagataat gcctgcggcg gtcagagtcg
     7381 cagtcagcaa tacagcagca aggctggagt agctgataat ggcgaacatc gcgccaatca
     7441 gcgcatccag cagaatatcg ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct
     7501 gggtgatcgg cgttacggcc tgcacaatca actccagcgc tagcaaaatc agcccaagac
     7561 caataccgac gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca
     7621 cgccgataaa aataagtaac ggtgacagcc aggataagtc gaaggtgaga atacgcgcca
     7681 ttagcgccgt cccgacatct gcccccagca caatgaccag agccggtgcg agggctacca
     7741 gatcctgagc gacgaacgag gtcaccagca tggtggtggc attactgctc tgtaccagtg
     7801 cggtaacgcc gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga
     7861 cagtacgcaa acgcgcgccg aagacgcgca ttacgccggt tcgaacaata tgagtccccc
     7921 agaccagcag ggcgacggca gaaagcaggt gaagcagcgt taacacgagt tcaggttctc
     7981 cttatcgtta tatgttcctg aagcaattga atgaccaaag gaccggaaac acgctgactg
     8041 gcatctttac tgtgcctttt gacattcgtt tttccggcta agattattcc agcctgtttc
     8101 cagtataagt gtttcatatt aataaagaga cagggcactc attgagcgcc ctgtttgttg
     8161 taaggaaaag tgacagattt gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc
     8221 agcgctcaac ttcggtaacg ctcatacctt tacggcgggc ataatcttca acctgatcgc
     8281 gctgaatttg tgctacagcg tagtacttgc tgtccgggtg gctgaagtac caacccgaaa
     8341 ccgatgcacc gggccacatg gcgaaagatt ctgtgagttt catgccagtg tgtttttcca
     8401 cttccagcag ctcccagatg gtggcttttt ccgtatgttc cgggcaggcc ggatagcccg
     8461 gtgccggacg gatgccctgg tagttttcgc ggatcagctc ttcgttgctg aggttctcgt
     8521 tcggcgcata gccccagtag actttacgca cacgctcatg gagatactcc gcaaaggctt
     8581 cggctaaacg gtcggcaagc gctttcacca tgattttgtt gtaatcatcg tgctgcgctt
     8641 caaaggcatc agccagtgcg tcctcttcca gcccgccagt cacggcaaat gcgccgatgt
     8701 aatctgcttt accagaaagc ttcggcgcaa cgaagtcagc gagacagtag ttagcgaagc
     8761 ctgttttttc ggtctgttga cgcagatggt ggctgacgtt gatcacatgg gtacgcgttt
     8821 cgtcacggta gatttcaatg tcatcgccca cacggtttgc cgggaacagg cccaccacgc
     8881 cacgcggatt cagcgttttc tcggcgctta atttatccag catgtcgttg gcgtctttaa
     8941 acagccgctg cgcctcaacg cccaccactt catcttccag aatgcgcgga tacttcccgg
     9001 ccagcgacca ggtcataaag aacggtgtcc agtcgatgta attacgcagc gtttcgatgc
     9061 tggcttcgac ttcctgcacg ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt
     9121 caaaagcgaa gtcgttatcg cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct
     9181 tgcgcccgtg ctgaatacgt acggtttcgt actccttgcg ggtacgagcg acaaaatcat
     9241 cgcgctgagt atcggaaagc agcgccgcca ccacaccaac ggtgcgcgag gcattctgca
     9301 catacaccgt cgggccgctg tagttctgct caattttcac cgccgtgtgc gcttttgagg
     9361 tcgtcgcgcc accgatcagt aacggaatgg tgaagccctg acgctccatc tctttcgcca
     9421 cgttaaccat ctcgtccagc gacggcgtga taagccccga aagtccaatc agatcagcat
     9481 tcacttcttt agcggtacgg agaatttttt ccgcaggcac catcacgccg agatcgacaa
     9541 tttcgtagtt gttacattgc agcaccacac caacgatatt tttaccgatg tcgtggacgt
     9601 cgcccttcac ggtggcgatc accatcttgc cgttggtttt gccctgctct ttgctggctt
     9661 caataaacgg ttcgaggtag gccaccgcct gtttcatgac gcgcgccgat ttgaccacct
     9721 gtggcaggaa cattttgcct tcgccaaaca gatcgccgac cacgttcatg ccgtccatca
     9781 acgggccttc aatcacttca atcgggcgcg tagcctgctg gcgggcttct tcggtatcct
     9841 gctcgataaa ctcagtaatg cctttgacca gcgagtattc cagacgttta ttcacttccc
     9901 acgagcgcca ctccgcctgc tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt
     9961 tctcggcaag ctccagtaaa cgctcagtgc catcatcgcg acgattaaga atcacatctt
    10021 ccaccgcgtc gcgcagttca gcgggcaggt cgtcgtaaat cgccagctgc ccggcattga
    10081 cgatccccat atccatgcca ttgcgaatag cgtagtagag gaacactgcg tgaatggctt
    10141 cgcgcaccgg atcgttgcca cggaatgaga aagaaacgtt agatacgccg ccggaaatca
    10201 gcgcgtgcgg cagttcgcgt ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt
    10261 tgttgtgctc ttcaatgccg gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt
    10321 ctggcgggaa gccaacctct tcggtgagga ttttgtacgc ccgacggcaa atctcgattt
    10381 tccgtgcgcg ggtatcggcc tgtccctgtt cgtcaaaggc cattaccacc actgccgcac
    10441 cgtaacgacg caagagcttc gcgtgatgga taaaggcgtc gacgccctct ttcatcgaga
    10501 tagagttaac aatgcctttg ccctggatac acttcagacc tttttcaatg acatcccatt
    10561 ttgaagagtc gatcataatc ggcacgcgag cgatatccgg ttcaccggca atcagattga
    10621 gaaaacgcac catcgccgct tcggcgtcga gcatcccttc atccatgttg atatcgataa
    10681 tctgcgcgcc gttttccacc tgctggcggg ccacatccag tgcctcgctg tatttctctt
    10741 ctttgatcag gcgcttgaac ttagcggaac cggtgacgtt ggtgcgttca cccacgttca
    10801 caaacaggct atcttcgcca atgttcagcg gctccaggcc ggacaaacgg caggctacgg
    10861 ggatttccgg cagtttgcgc ggcgctaatc cttctactgc acgactcatc gctgcaatat
    10921 gttgtggcgt ggtgccacag cagccgccga cgatattgag aaaacccgct tgcgcccatt
    10981 cacgtatctg ttttgccatc gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta
    11041 gcccggcgtt cgggtgcgcg gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt
    11101 actggcgcag ttcatcgggc cccagcgcac agttcaggcc aaaggtcaga gcttcggcgt
    11161 ggcgcaatga gttgtaaaat gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt
    11221 cggtgatggt gccggagatc ataatcggca gctcaacgcc cagcgcttca aactccgttt
    11281 tcaccgcaaa aaccgccgct ttggcgttaa gggtgtcgaa aacggtttca atcaggatca
    11341 gatccgcgcc accttccacc agcgctttgg tggactctcg ataagccgcc accagcccgt
    11401 caaaagtgat attacgaaat gccggatcat ttacgtccgg agaaatagac gccgtgcggt
    11461 tggtcgggcc gagaacaccg gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact
    11521 catcagcaca agctcgcgcc agtttcgccg ccgcaaagtt gatttccgcc gacagggatt
    11581 ccatctggta atccgccatc gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat
    11641 ccgcgcccgc ttcaaagtag gcgttgtgga tagcggcgat cacttccggt ttgctgagca
    11701 ccagcaggtc gttgttgcct ttgagatcgc atggccagtc ggcaaagcgt tcaccacgaa
    11761 aatcggcttc gttcagtcga tagctctgga tcatggtgcc cataccgccg tccagcacca
    11821 gaatacgttc atttaactgc gcacgcagtt gttccacttt gctgctcaca cttgctcccg
    11881 acacgctcaa cccagattta ataaaaattc aacaaaccat actggcataa acgcatctgt
    11941 ggtaaaagcg accaccacgc aacatgagat ttgttcaaca ttaactcatc ggatcagttc
    12001 agcaactatt gcattagcta acaataaaaa tgaaaatgat ttccacgata cagaaaaagg
    12061 aggctgtcat ggtcgcaccc attcccgcga aacgcggcag aaaacccgcc gttgccaccg
    12121 caccagcgac tggacaggtt cagtctttaa cgcgtggcct gaaattactg gagtggattg
    12181 ccgaatccaa tggcagtgtg gcactcacag agctggcgca acaagccggg ttacccaatt
    12241 ccacgaccca ccgcctgcta accacgatgc aacagcaggg tttcgtgcgt caggtcggcg
    12301 aactgggaca ttgggcaatc ggcgcacatg cctttatggt cggcagcagc tttctccaga
    12361 gccgtaattt gttagcgatt gttcacccta tcctgcgcaa tttaatggaa gagtctggcg
    12421 aaacggtcaa tatggcggtg cttgatcaaa gcgatcacga agcgattatt atcgaccagg
    12481 tacagtgtac gcatctgatg cgaatgtccg cgcctatcgg cggtaaattg ccgatgcacg
    12541 cttccggtgc gggtaaagcc tttttagccc aactgagcga agaacaggtg acgaagctgc
    12601 tgcaccgcaa agggttacat gcctataccc acgcaacgct ggtgtctcct gtgcatttaa
    12661 aagaagatct cgcccaaacg cgcaaacggg gttattcatt tgacgatgag gaacatgcac
    12721 tggggctacg ttgccttgca gcgtgtattt tcgatgagca ccgcgaaccg tttgccgcaa
    12781 tttcaatttc cggaccgatt tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga
    12841 tggtgattaa agcggcgaag gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc
    12901 tggcgggcag aggcaatatt ctgcccatca tacctgagtg gcaatagaat aagggtgtct
    12961 gttaatcgca ttgacgccaa aataacttaa tgtcatacac ttcacacact tttattaact
    13021 cagcattatt tttaaacatc aaaccactta atcattaaca aaatcgcaag atctcattcc
    13081 accgaaactt ttacggctgc attatgagat tgagcgattt atgatatttc tttagttgta
    13141 atcatattaa attttttcac tgttctgata gttaaaattc aagacatcaa taaacaatga
    13201 gatatttaaa tgattactcg catccctcgt agttctttct ctgcaaatat taataataca
    13261 gcccagacaa atgaacacca aaccctgagt gaattgtttt ataaagaact cgaggataaa
    13321 ttttctggca aggagctggc gacgcctcta ttaaaaagct tctcagagaa ctgtagacaa
    13381 aatggtcgtc atatctttag caacaaggat tttgtcatta aattttccac gtccgtctta
    13441 caagctgata agaaagaaat tacgataatt aataaaaacg aaaacacgac acttactcaa
    13501 accattgccc caatatttga agaataccta atggaaattt tacctcaacg ctcagacact
    13561 cttgataaac aagaattaaa cctaaaatca gatagaaaag aaaaagaatt cccaagaatt
    13621 aaacttaatg gtcaatgtta ttttccgggg cgaccccaaa accgtatagt atgccgacac
    13681 attgctgcac aatatattaa tgatatttat cagaatgttg attacaaacc ccatcaagat
    13741 gattactctt cagctgaaaa atttctcacg cacttcaaca aaaaatgcaa aaaccagact
    13801 ttggcgttgg tttccagccg tcctgagggg cgttgcgttg ctgcctgcgg tgatttcggg
    13861 ctagttatga aagcatattt tgacaagatg gaatcaaatg gcatcagtgt tatggcagcc
    13921 atattactgg tggataacca tgctttgacg gtccggctaa gaataaagaa cacaactgaa
    13981 ggatgtaccc attacgtggt ttcggtttat gatcctaatg taactaacga taaaataaga
    14041 attatgagcg aaagcaaaga ggatattaaa cactattctc tgatggattt tatgaatgta
    14101 gattatagcc tcctgaaatg gtcaaatgat catgttatta accaatctgt tgcaataatt
    14161 ccagcacttc cgaaagaaca gctattgatg ttaaaaggat ctgtggatga aataacccct
    14221 ccattatcac cagcaacgat gaatttgcta atggcaattg gtcagaatca ccaacttacg
    14281 caactgatga ttcagctcca gaaaatgcca gaactacata gaacagaaat gttgactgcc
    14341 tataatagtg gacatatgaa cgttattaat actattttta acgcattacc cactctgttt
    14401 aatacgttta aattcgataa aaaaaatatg aagcccctcc tcctggcaaa taattctaat
    14461 gaatatcccg gtttgttttc agcgatacag cataaacaac aaaatgttgt agagacggtt
    14521 tatcttgctt tatctaacca tgcacgcctg tttggattta ccgctgaaga tattatggat
    14581 ttttggcaac acaaagcccc acaaaaatac tctgcctttg agttggcttt tgaattgggt
    14641 caccgggtta ttgctgaatt aatccttaat acattaaata agatggctga aagctttggc
    14701 tttacggata accctcgata cattgcggag aaaaattata tggaagcttt actcaaaaaa
    14761 gcatctcccc ataccgtacg ctaaatcttt gcctgcgccg atacgcataa acatcttcca
    14821 catgcccttc acgtatgcgg ttttgtagtg cgcgccagta atcagcgcgg aacaggtcgg
    14881 cgtgcatctc ttcaaacagc ggaccaatac gcgggtcggc gcatagccag tggcgaaact
    14941 cttccgggaa aacatcgccc ggcgagacgc tgtaccacgg ttcgctggca agttcgtctt
    15001 ccggatagcg cggcggcggg atgtcgcgga aattcacttc cgtcatgtag caaatttcat
    15061 cgtaatcata aaaaaccaca cgcccgtgac gggtgacacc gaagttttta aacagcatgt
    15121 cgccaggaaa aatgttagca gcggcaagct ggcgaatagc gttaccgtat tcttcaatgg
    15181 cgtcgcgcaa ctgctgacct tccacttgtt ccagccagat attgagcggc accatccgcc
    15241 gctcgatata gagatggcga attacaatct gctcaccaag atcggtgatt ttttccgctg
    15301 cttcctggag cagtaattcc attaatgccg gggaaatatg ccgcttctcc agcacaaagt
    15361 tttcaaactc ctgggtgtcc gccattcggc ccacacggtc gtgctctttc accagttgat
    15421 agcaggcacg aacgtgagcg gcagacatct ctttctgcgg cgcgaacttg tctttgatga
    15481 ctttgaatac ccgatcaaag cccggcagcg taaataccaa catcaccatt ccacgaatac
    15541 ccggcgcttc aatgaactgc tcattacagc cctgtagata aacgagatat tcgcggtagc
    15601 tttcggtttt ggcgtgcttc tggcagccga tagccatata caattcagcg gtggttttac
    15661 ccggcagaat ttcccgtagc cactcgacca acgctgcggg cagcggcgcg taaaccataa
    15721 aataagaacg cgcgaagcca aagacgatgc tcgcttcggc ggtggtcgtc aggcaggtat
    15781 caataaataa ctcgccatcg tccgtctggt ggatcggcag caaaaagggc aatgtgccgg
    15841 aagacgtgat cagtttgccc accagccagg cggctttatt gcggtaaaac agttcgttcg
    15901 ccacctgtaa atgactttcc gcgaggttgt ctgtccccag cgtttccgtc agatggcgaa
    15961 tgatgtaatg aatgtcacgg cttttattct gccaacgcag acgcagcggc aggtcgctga
    16021 taacgcgcat cagcagagat tcccagccgt gatcggggtg aaagtctttc gccagcgggc
    16081 gcggaatagt acgaaaacgg cgctctggct gggagctaaa gataaaaagc cgctcgggag
    16141 taagcgagcg gtggtcaaat aaccgacagt acacggagtt aaaaaagctc tccgcaatct
    16201 cgaagcgcgg gtaatccggc aacagccgcg tgtaatgctc tttaacgcgc agtaaaaatg
    16261 ccgcgtccgt actttggccg ttagtaatgc agcgcagttg ctccacgacc agaccaacgt
    16321 gatgatcgta aagatggata cggtttttca tcgcctgctg gacagcatgc cagtcggcct
    16381 gttcgaaacg ctgctgcgcc ccggaagtca cttcga
//
LOCUS       DAJOSY010000094        16403 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJOSY010000094 DAJOSY010000000
VERSION     DAJOSY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.094"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..370
                     /locus_tag="OHN27_003304"
     CDS             <1..370
                     /locus_tag="OHN27_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCQ2287689.1"
                     /translation="IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNK
                     ATFKKLLVNRSGNGLVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNN
                     ANNWYLTIIDDHGNYISDKI"
     gene            386..2866
                     /locus_tag="OHN27_003305"
     CDS             386..2866
                     /locus_tag="OHN27_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2287690.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSNYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGSVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYQENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            2882..3916
                     /locus_tag="OHN27_003306"
     CDS             2882..3916
                     /locus_tag="OHN27_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCQ2287691.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKKEDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNIILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKIKSGALE
                     ATALINVKYD"
     gene            complement(3998..4336)
                     /gene="rcnB"
                     /locus_tag="OHN27_003307"
     CDS             complement(3998..4336)
                     /gene="rcnB"
                     /locus_tag="OHN27_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCQ2287692.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(4555..5379)
                     /gene="rcnA"
                     /locus_tag="OHN27_003308"
     CDS             complement(4555..5379)
                     /gene="rcnA"
                     /locus_tag="OHN27_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCQ2287693.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEHHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            5500..5772
                     /gene="rcnR"
                     /locus_tag="OHN27_003309"
     CDS             5500..5772
                     /gene="rcnR"
                     /locus_tag="OHN27_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCQ2287694.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5864..5960
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5995..6783
                     /gene="thiM"
                     /locus_tag="OHN27_003310"
     CDS             5995..6783
                     /gene="thiM"
                     /locus_tag="OHN27_003310"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCQ2287695.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            6780..7580
                     /gene="thiD"
                     /locus_tag="OHN27_003311"
     CDS             6780..7580
                     /gene="thiD"
                     /locus_tag="OHN27_003311"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCQ2287696.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            7645..8463
                     /locus_tag="OHN27_003312"
     CDS             7645..8463
                     /locus_tag="OHN27_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCQ2287697.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            8515..9261
                     /locus_tag="OHN27_003313"
     CDS             8515..9261
                     /locus_tag="OHN27_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCQ2287698.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAEQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(9235..10200)
                     /locus_tag="OHN27_003314"
     CDS             complement(9235..10200)
                     /locus_tag="OHN27_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCQ2287699.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEITAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(10197..11201)
                     /locus_tag="OHN27_003315"
     CDS             complement(10197..11201)
                     /locus_tag="OHN27_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCQ2287700.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(11198..12475)
                     /locus_tag="OHN27_003316"
     CDS             complement(11198..12475)
                     /locus_tag="OHN27_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HCQ2287701.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            12732..13784
                     /gene="fbaB"
                     /locus_tag="OHN27_003317"
     CDS             12732..13784
                     /gene="fbaB"
                     /locus_tag="OHN27_003317"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCQ2287702.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAVTIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            14091..14945
                     /gene="gatY"
                     /locus_tag="OHN27_003318"
     CDS             14091..14945
                     /gene="gatY"
                     /locus_tag="OHN27_003318"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCQ2287703.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14974..16241
                     /gene="gatZ"
                     /locus_tag="OHN27_003319"
                     /pseudo
     CDS             14974..16241
                     /gene="gatZ"
                     /locus_tag="OHN27_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            16251..>16403
                     /locus_tag="OHN27_003320"
     CDS             16251..>16403
                     /locus_tag="OHN27_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCQ2287704.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGI"
BASE COUNT         4345 a         3873 c         4176 g         4009 t
ORIGIN      
        1 cattttttat ctgaatgttc tggatattcc accaaatagt ccagagcaag aaggtaagaa
       61 tgcactgaag tttgcgatgc aaaacagaat taagttgttt taccggccag cgggtattgc
      121 tccggtaaat aaagcgacct ttaaaaagtt actggtaaat cgcagtggca atggattggt
      181 gataaaaaat gactcagcta attgggtgac gatttcggat gtcaaagcta ataatgttaa
      241 agtcaattat gaaactatta tgattgcccc cttagaaagt cagagtgtta atatcaaaag
      301 taataatgca aataactggt atctgaccat tatcgatgac cacggcaact atattagtga
      361 caaaatttaa ttgcaggagc tgcctatgtt gagaatgacc ccacttgcat cagcaatcgt
      421 agcgttattg ctcggcattg aagcttatgc agctgaagaa acctttgata cccattttat
      481 gataggtgga atgaaagacc agcaggttgc aaatattcgt cttgatgata atcaaccctt
      541 accggggcag tatgacatcg atatttatgt caataagcaa tggcgcggga aatatgagat
      601 tattgttaaa gacaacccgc aagaaacatg tttatcaaga gaagttatca agcggttagg
      661 cattaatagc gataacttcg ccagcggtaa gcaatgttta acatttgagc aacttgttca
      721 gggtgggagc tatagctggg atatcggggt ttttcgtctc gatttcagtg tcccgcaggc
      781 ttgggtggaa gaactggaaa gtggctatgt tccaccggaa aactgggagc ggggtattaa
      841 tgcgttttat acctcttatt atgtgagtca gtattacagc gactataaag cgtcgggtaa
      901 caacaagagt acatatgtac gttttaacag cgggttaaat ttactggagt ggcaactgca
      961 ttctgatgcc agtttcagta aaacaaataa caatccaggg gtgtggaaaa gcaataccct
     1021 gtatctggaa cgtggatttg cccaacttct cggcacgctt cgcgtgggtg atatgtacac
     1081 atcaagcgat atttttgatt ctgttcgctt cagcggtgtg cggttgtttc gtgatatgca
     1141 gatgttgcct aactcgaaac aaaattttac gccacgggtg cagggaattg ctcagagtaa
     1201 cgcgctggta actattgaac agaatggttt tgtggtttat cagaaagagg ttcctcctgg
     1261 cccgtttgcg attacagatt tgcagttggc cggtggtgga gcagatcttg atgtcagcgt
     1321 gaaagaggcg gacggctcgg taaccaccta tctggtgcct tatgcagcgg tgccaaatat
     1381 gctgcaaccc ggcgtgtcga attatgattt tgcggcgggt cgtagccata ttgaaggggc
     1441 gagcaaacaa agtgattttg tccaggcggg ttatcagtat ggttttaata atttattgac
     1501 gctgtatggt ggctcgatgg tcgcgaataa ttattacgcg tttactttgg ggactggctg
     1561 gaatacacgc attggtgcca tttccgtcga tgccacgaag tcgcatagta aacaagacaa
     1621 cggcgatgtg tttgacgggc aaagttatca aattgcctac aacaaatttg tgagccaaac
     1681 gtcgacgcgt tttggtcttg cggcctggcg ttattcatcg cgtgattacc ggacatttaa
     1741 tgatcacgtt tgggcaaaca ataaagataa ttatcgccgt gatgaaaacg atatctatga
     1801 cattgccgat tattaccaga acgattttgg ccgtaaaaat agcttttctg ccaatatgag
     1861 ccagtcattg ccagaaggct gggggtctgt gtccttaagt acgttatggc gagattactg
     1921 ggggcgtagc ggcagcagta aggattatca gttgagttat tccaataact ggcggcggat
     1981 aagctatacc ctcgcggcaa gccaggcgta tgacgagaat catcatgaag agaaacgttt
     2041 taatattttt atatcgattc cctttgattg gggtgatgac gttacgacgc ctcgtcggca
     2101 aatatatatg tctaactcaa cgacgtttga tgatcagggg tttgcctcaa ataatacggg
     2161 attatcagga accgttggaa gccgggatca gtttaactat ggggtcaacc tgagttatca
     2221 gcatcaggga aatgaaacga cagctggggc gaatttgacc tggaacgcgc cggttgcgac
     2281 agtgaatggc agttatagtc agtcgagtac ttatcgacag gctggagcca gtgtttcagg
     2341 gggcattgtc gcctggtcgg gtagcgttaa tctggcgaac cgtctttccg aaacgtttgc
     2401 tgtgatgaat gcgccaggaa ttaaagatgc ttatgtcaat gggcaaaaat atcgcacaac
     2461 aaaccgtaat ggagtggtga tatacgacgg aatgacacct tatcaggaaa atcacctgat
     2521 gctggatgtg tcgcaaagcg atagcgaagc agaattacgt ggcaaccgga aaattgccgc
     2581 cccttatcgc ggcgcggttg tactggttaa ttttgatacc gatcagcgca agccctggtt
     2641 tattaaagcg ttaagagcgg atggtcaacc attaatgttt ggttatgaag tcaatgatat
     2701 ccatggtcat aatattggcg ttgtcggcca gggaagccag ttatttattc gcaccaatga
     2761 agtaccgcca tcggttaatg tggcaattga taagcaacaa ggactttcat gcacaatcac
     2821 cttcggtaaa gagattgatg aaagtagaaa ttatatttgc cagtaatagt caggtggttc
     2881 tatggaaatt cgcataatgc tatttatatt aatgatgatg gttatgcctg tgagctatgc
     2941 ggcatgttat agtgagttat ctgttcagca caatttggtt gttcaggggg attttgcact
     3001 tactcaaaca caaatggcga catatgagca taattttaat gattcgtcat gcgtgagtac
     3061 aaatactatc acccctatga gcccgtcgga tattattgtt ggactttata acgataccat
     3121 taaattaaat ttacattttg aatggaccaa taaaaacaac atcacgttgt caaataatca
     3181 gaccagtttc accagtggtt attcggtaac ggtgacacct gcggccagta atgcaaaagt
     3241 gaatgtttct gcggggggcg gcggttcagt gatgattaat ggagttgcga cattatccag
     3301 tgcttcatca tcgacacgcg ggagtgccgc agtacaattt ctactgtgtt tattaggtgg
     3361 caagtcatgg gatgcatgtg taaatagcta cagaaatgca ttggcacaaa atgcaggtgt
     3421 ctattccttt aatctgacat tgtcatacaa cccgataacc acaacctgca aaccggacga
     3481 tttattaatt actttagaca gtattcccgt ttcacaatta ccagcgacag gtaacaaagc
     3541 aacaataaat agtaaaaaag aggatattat tctgcgttgt aaaaatttat taggtcaaca
     3601 aaatcaaaca tcacggaaaa tgcaggtgta tttatcaagt tctgacttgt taaccaacag
     3661 caacataata ctgaaaggtg cggaagataa tggcgtagga tttattcttg aaagtaatgg
     3721 ttcgccagtc acacttttaa atatcactaa cagcagtaaa ggatacacaa atttaaaaga
     3781 aattgcggca aagtcaaaac ttacagatac aacggtttca attccgataa cagccagtta
     3841 ctacgtctac gatacaaata aaattaaatc tggcgcactg gaggcaaccg cattaatcaa
     3901 cgtgaaatac gactaataac aagcaagacg agcaagtggc taataataaa aaataacaag
     3961 gtgtctgcat ttccccctca tgatgagggg gcttttttta gcgatgataa aaaatctcac
     4021 cgtcgtaggc tttaatgatt ttaccgtcgg tgtcgctgat caacacgtac gcgccaccca
     4081 tataggtcca gtgcgtcccg gcatcaggcg cgggcaggtt acgtaactgc cactgcttaa
     4141 tgttgtactc atcggtgcgg tacatctctg ggacggtatc gcctggtttg aaatgcttaa
     4201 agtcagcaat gaaactgctt aactcatact tagaaattcc cgaggtattg gtcgaacccg
     4261 cggtggctgg tgcggcccag gcggcactgg taaccagcaa aagcgcaccc agcaacatct
     4321 tatttttaat ggtcataaac tctccacttt ggggatctgt aatatcagtc gcatatcttt
     4381 cctgccatac aatataactg acaagcgata ttttgttagt gatcccatga tttcgtaaaa
     4441 agtgtaaatg aaaggatgcc ctaaaagcat ccttagcgag cagagagtca gcggaaatag
     4501 ctgatttaag aatcatcctg ggctgaagat aacatgcaag cctaaaggat tgagttatcg
     4561 cattatgccc atgaagccat gtacgcccat atacacaccg actaagccaa tcaacagact
     4621 ggaaaaatag ggggcgcgtt tagcgagagt gttaaatccg ctccagcgtt ttgcgacctg
     4681 ctgaacgctg attgctgcgc caacgcctac ggtgacaagc gttaacgcca ggccaatgct
     4741 gaaactgacg accagtgttg cgcccagtgt cagggctttc aactgaatgc atatcaacag
     4801 cacggtaatt gctgccgggc aggggataag gccaccggtt aagccaaata acaaaatttg
     4861 ccagttggtg acctctctac catcaaagcg tcgtttaatg tcattggcat gggctcgtgc
     4921 atgggcatcc tgatactcgc catgttcgtg atggtgatga tgtccatggt cgtggtgatg
     4981 ttcgtgatcg tgatgatgat gctcataatc atgcccgtgc atattctcca gccagttgcg
     5041 ttcgccgcgc caggtacgcc agaacatcca gaacgcggtg ctaataatga tcactgcgga
     5101 aatcagctgg agccacggtt ctgctgattg agcagtaaag cgcttgctga tcaccatccc
     5161 gccaaaggca attaaccaga ccactgcggt atgcgaaata gttgctgcca gtccgagcat
     5221 caccgcttgt ttaatggtgc ctttgatggc gatgataaac gccgccatca tcgtttttga
     5281 gtgccctggt tccaggccat gaagcgcacc aagtaagatg gcgctgggga tgaagaacca
     5341 ggcgtttcct tgctgaagaa gagttgtaaa ttcggtcatg ataataattc ttagtattaa
     5401 ttcggcaatc tgattctact cccccccagt acctgatact accccccagt agattaatag
     5461 tgctatgatt tttcatattc ttgttaacca ggtgttgcca tgtctcatac aatccgtgat
     5521 aaacagaaac tgaaagcgcg tgccagtaag attcagggcc aggtcgtggc gctcaagaaa
     5581 atgctcgacg agccgcacga atgcgctgca gttttacaac agattgctgc tatccgtggc
     5641 gcggtaaacg gtctgatgcg ggaagtgatt aaaggtcatc tgacggaaca catcgttcac
     5701 cagggggatg agctaaaacg tgaagaagat ctggatgtcg ttctgaaggt gctggattca
     5761 tatatcaaat aatttattaa cgcgattgta aaactgccgt ttttcctcgt ttacaacgcg
     5821 tgcgctggac attaccatcc tcctctgcga tttatcatcg caaccaaacg actcggggtg
     5881 cccttctgcg tgaaggctga gaaatacccg tatcacctga tctggataat gccagcgtag
     5941 ggaagtcacg gaccaccagg tcattgcttc ttcacgttat ggcaggagca aactatgcaa
     6001 gtcgacctgc tgggttcagc gcaatctgcg cacgcgttac acctttttca ccaacattcc
     6061 cctcttgtgc actgcatgac caatgatgtg gtgcaaacct ttaccgccaa taccttgctg
     6121 gcgctcggtg catcgccagc gatggttatc gaaaccgaag aggccagtca gtttgcggct
     6181 attgctagtg cgttgctgat taacgttggc acgctgacgc agctacgcgc acagtcgatg
     6241 tgtgccgccg ttgagcaagc aaaaagctct caaacaccct ggacgcttga tccagtagcg
     6301 gttggtgcac tcgattatcg ccgccgtttt tgtctggaac ttctgtctca taagccaacc
     6361 gccatacgtg gtaatgcttc ggaaatcatg gcattagctg gcattgctaa tggtggacgg
     6421 ggagtggata ccactgacgc cgcagctaac gcgatacccg ctgcacaaac actggcacgg
     6481 gaaactggcg caatcgtcgt ggtcactggc gagatggatt atgttaccga tggacatcgt
     6541 atcattggta ttcacggtgg cgatccgtta atgaccaaag tggtaggaac tggctgtgca
     6601 ttatcggcgg ttgtcgctgc ctgctgtgca ttaccaggcg atatgctgga aaatgtcgca
     6661 tctgcctgtc actggatgaa acaagccgga gaacgcgcag tcgccagaag cgaggggcca
     6721 ggcagttttg ttccacattt ccttgatgcg ctctggcaat tgacgcagga ggtgcaggca
     6781 tgaaacgaat taacgctctg acgattgccg gtactgatcc gagtggtggt gcggggattc
     6841 aggccgatct taaaaccttc tcggcacttg gcgcttatgg ttgctcagtt attactgcac
     6901 tggtggcgca aaatacccgt ggcgtacagt cggtgtatcg cattgagcct gattttgtcg
     6961 ccgcccagct cgattcggtg ttcagcgatg tgcgaatcga caccactaaa atcggtatgt
     7021 tggcggaaac cgatattgtt gaagcggtgg cagaacggtt gcaacgttat cagatccaaa
     7081 acgtggtact cgacaccgtt atgctggcaa aaagcggcga cccgctgctt tcaccttcgg
     7141 cggttgctac gctgcgcagt cgattattgc cacaggtttc attaataacg ccaaacttgc
     7201 ccgaagctgc cgccttgctc gacgcgccac acgcgcgcac cgaacaggaa atgctggaac
     7261 aagggcgatc gctgttggcg atgggctgtg gcgcagtgct aatgaaaggt ggtcatctgg
     7321 atgatgagca aagcccggac tggctgttta cccgcgaggg tgaacaacgg tttaccgcac
     7381 cgcgcattat gaccaaaaac acccacggca ctggttgtac gctctctgcg gcgttggctg
     7441 cactacgccc gcgccataca aactgggctg acaccgtaca ggaggcaaaa agctggcttt
     7501 catcagcatt agcccaggcc gacacgctgg aagttggtca cggtattggt ccggttcacc
     7561 acttccacgc ctggtggtga gcaccgtact atagctgctt tggttattgt cagcctttgg
     7621 cactgacatt cagggatgaa accaatgcag ctaagaatta ccagtcgaaa aaaactcacc
     7681 gctctattat gcgctcttgg gctaatttcc atcgttgcaa tttatccgcg tcagacggtg
     7741 aattttttct attcgacagc agttcagatt acagactaca tccatttcta cggttatcgc
     7801 ccggttaaat ctttcgctat tcgcattcct gccagttaca ccattcacgg aatagatgtt
     7861 tctcgctggc aggagcggat cgactggcag cgggtggcaa aaatgcgcga caacggtatc
     7921 cgcttacagt ttgcttttat taaggcgacg gaaggcgaaa agctggtgga cccgtatttt
     7981 tcgcgtaact ggcaactaag ccgcgaaaat ggcctgctgc gcggggcgta tcattatttc
     8041 tccccgtcgg tagcagcgcc ggttcaggca cgattatttc tgcaaacggt ggatttctca
     8101 caaggcgatt tccctgcggt actggacgta gaagaacggg gaaaattatc ggcaaaagaa
     8161 ttacgcaaac gagtaagtca gtggctaaaa atggtcgaaa aaagtacggg aaaaaagccg
     8221 attatttact caggagccgt tttttatcac accaatctgg cgggctattt caatgaatat
     8281 ccgtggtggg tggctcacta ttatcaacgt cgtccggaca atgacggcat ggcctggcgc
     8341 ttctggcagc attccgaccg tggacaggta gatggcatca atggtccggt ggattttaat
     8401 gtatttaatg gcacggtgga ggagttgcag gcattcgttg atgggattaa agaaacgcct
     8461 taaataagaa gcagtgatat actcccacca gactccccgg caggaaaaag acaaatggaa
     8521 caagcgcata cccagctgat tgcccaactg aacgagcgta ttttagcggc ggataacacg
     8581 ccgctttata ttaagtttgc cgaaacggta aaaaatgccg tgcgcagcgg ggtgctggag
     8641 catggcaata ttttgcccgg tgagcgtgat ttaagtcagt taaccggcgt ttcgcgcatt
     8701 acggtgcgca aggcgatgca ggcgctggaa gaagagggtg tggtgacgcg ttcgcgcggt
     8761 tacggcacgc agatcaacaa catcttcgaa tactcgttga aagaagcgcg aggtttttct
     8821 cagcaggtgg tattgcgcgg gaaaaagccc gatacgctat gggttaacaa gcgtgtcgtg
     8881 aaatgtcccg aagaagtcgc ggagcagctg gcggtcgaag caggaagtga tgtctttttg
     8941 cttaagcgta ttcgctatgt cgatgaagaa gcggtatcga ttgaggaatc gtgggttccg
     9001 gcgcatttaa ttcatgatgt tgatgccatc gggatttcac tttatgacta tttccgcagc
     9061 cagcacattt acccacaacg tacgcgttcc cgcgttagcg cccggatgcc ggatgccgag
     9121 tttcagtcac atattcagtt agatagcaaa ataccggtgc tggtgatcaa gcaagtggcg
     9181 cttgaccaac agcaacggcc tattgagtac agcattagcc actgtcgcag cgatctatac
     9241 gtttttgtgt gcgaggagta gttcctcgcg cgttggcgca caatcaccgc cccgatgccc
     9301 gacaacccac gacgccactg cattgcccag taatacggca tccgccagtg gcagaccaga
     9361 ggccagcccg gcaagtacgc cacctgcatg actgtcgccc gccccaatgg tgtctacaac
     9421 ttgcgttgga aatgccggaa tgcagccaga agcgtcgttg ctgaaatacc atgcgccttc
     9481 tttatcgaga cgaacgatca acggcgcggc aaatttctcc tgccattgct cgccaagtgt
     9541 tgttatctct gcggataaag caaaacgttc ggcggtaatc tcagcctctt gacgattgag
     9601 cgacactaaa ggtcgacagg ccatgatccg cgccagtaat gcatccggga tatcgccaat
     9661 acgtgggcca aaatcgataa acggcgtcac gtcttgcagc tcttccagcc attccactaa
     9721 caattcgccg cagggcgagg ccagttgata accggaaaaa tagagcaggt tgccaggcgc
     9781 aacggttaat cgcgccagcc actggcgatt ccactgattt tccacaccgc tgaatgacat
     9841 aaaagtacgt tcgccatccg gctcaaccag cgccagacac cagccgttat cgccttcggc
     9901 gttatcgatc aggctgatta acccctcttt cgccatccgg ttgcgaataa tctccgccca
     9961 taccccctga ccgagcggca aggcattacc cgcttcgatg ccgaggcgct ttaacgccac
    10021 ggcaatattc agtgcgcagc cgccaacgtt aacgctctgc tgtttcagtt cgatatcgca
    10081 cccacgccag gggagggcat aagcgtcggc gatcacgtca atgaccgccg cgccgacgac
    10141 catcaccgac tgacgcgtgg ttaattcagg cagcagcgtg tgtaatcgag cgccgctcat
    10201 accgcctccc gttgttgacg atatttcgcc agcgctgtgg catagcggtt gaaatcaagc
    10261 tgatttaccg catccagctc cgcctttaac gcaggatcga tagcgttaat gccatgcaac
    10321 gcgccgcaga ttgccgtcgc catagcaccg atggtgtctg tatcgccgcc aaggttagcg
    10381 cagaggacgg cgcaacggtt cgggtcggtt tgtgccagtt caaccagcgc aatggcgcac
    10441 ggaacggact caatagtgct ggtacctgcg ccaacgacct ggtaaagctg ttcgctggcg
    10501 gattcggtgc cgtcggcatt gcgcacaatt ttcagcgcaa tctccagccg cgccgccagt
    10561 gaggcgctga acgtggtggt gcgtttttgc tgggcatgtc gcgcaattga aggcagtgaa
    10621 tcaacaatcg ctgaccagct ttctccgtca atagcacgag aaatcgccca tgcgatgact
    10681 accgcacctg caactgccag atcggattta tgggtcgggc tcgacgccag cgccacatca
    10741 tcaataaagg aatcaacatc gcgcgccgga agcaaacaac ctaatggcga gacgcgcatc
    10801 gctgcgccgt tggttacgcc gttgttttcc agttcagcaa cgggtttacc gtcgcgaatg
    10861 gcgttaagcg caatcttcga ggtcggacct agcacgtttt tgttgaaggc gtcgaaacgc
    10921 agcgcccagt cgaggatatt acgcccaatc agatccggat cgatcttgcc tttacgttcc
    10981 agcaacgcat ccgccagaca cagcgccatc gaggtatcgt cggtgaattc ggcgcggtta
    11041 aaataacagg ccgcgttatt ctcctttggt ccaggcagaa aacggtcaat ccagccaaag
    11101 tgtgctttaa cgcggctgcg tggccaaagc tcggagggca tccccatcgc atcccctaac
    11161 gcctgcccat aaagagcacc gagaatacgt tctgttttca tttaacttcc ccttgtgtca
    11221 acgcaatatc gcgatcatcg accttgatag ccgtaatttc gttgtcggat tcgcggaaaa
    11281 aaatcatgaa cagcacggcg ataatggcaa tcatcaccgc gccaaaagtc cacatcccgg
    11341 accagttgaa agtcagtccg tttaccggtt cctgataagc gaacatcttt tccatcatca
    11401 caccgccaag acgatagccg agcaaactgc cgaagccctg gcagcagagc gtgatcagcc
    11461 cctgcgcagc ggtacgcata tgcacggggg cttttttatc gacatagatg taagcggtaa
    11521 cgtagtaaaa atcgtaactt acgccgtgaa gcaaaatacc gaggaacagt aacgcgtagg
    11581 tgaaatattc atccgcacta ccgtaaataa agaagccata gcggatcgca gcggtgacca
    11641 gaccaagcaa taataccttt ttgataccaa agcgtttagt gaaaaacggc aatgccagca
    11701 taaagaagat ttcagagaac tggccgagcg tcatccagcc ggtggcgttt ttcatgccaa
    11761 cttcggtcag ataaccgttg gcaaagatgt aatagaacgc caatggcatc gcaaacagga
    11821 atgaacagaa gaaaaagacg aggaagtttt tatcgcgcag caggatcagc gcatccaggc
    11881 cgagcatgac tttaatatcc attttgccgg tgctttttgg tggcgtgtcg ggcaggaaaa
    11941 acgcaaacac accgagcaga gcagaacttc cggcggtaat cagcagcggg atgttagtcg
    12001 gtgagatatc ggcatacccc agtatttgcg gcaagaaacc acatgccaga ccggaggcaa
    12061 tccagccgat agtgcccatc acacgaatgc gcgggaaatc acgctccaca tccggcacgt
    12121 tggcaaaagc gatgctgtta gtcagcgcaa tggtcggcat ataggttaga gagtaagcca
    12181 gcagtaacgg aaagaacccg gcaaaagtgg tctgttgcgc agcgaaatac atcagcagcg
    12241 cgcctgcgaa catcaatacc gccagcactt tttgcgccga gaaaaagcgg tcagtgatgg
    12301 agccaaccag aattggcgac aggatcgccg caatggcggt acaggcatac gaccagccaa
    12361 tttctccggc actaaaaccg cttttactta accacaacca caatggcaca aaccacgcgc
    12421 cccagataaa ccattcaaca aacatcatga acgacagctt tgctgttgtt ttcatctttt
    12481 aattccttca tgacaggtaa tgtaaggtac gcgatgacaa taccacttaa taataccttt
    12541 taaatacctt tgcggtgaaa acttgaccgc gctaacattt tttctgatga atcgagccaa
    12601 cagaaaacgc tgaaaaaaca tccaaaagat ggaaaaactc gcccgtaacg accaggatta
    12661 tgattgcccg caaaaggcgg gccaggacaa tccatagccg atatccaatc ggaatttacg
    12721 ggagcatagt aatgacagat attgcgcagt tgcttggcaa agacgccgac aaccttttac
    12781 agcaccgttg tatgacaatt ccttctgacc agctttatct ccccggacat gactacgtag
    12841 accgcgtaat gattgacaat aatcgcccgc cagcggtgtt acgtaatatg cagacgttgt
    12901 acaacaccgg gcgtctggct ggcacaggat atctttctat tctgccggtt gaccagggcg
    12961 ttgagcactc tgccggagct tcatttgctg ctaacccgct ctactttgac ccgaaaaaca
    13021 ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc atcaacttac ggcgtgttgg
    13081 cgtcggtatc gcggcgctat gcgcatcgca ttccatttct cgtcaaactt aatcacaacg
    13141 agacgctaag ttacccgaat acctacgatc aaacgctgta tgccagcgtg gagcaggctt
    13201 tcaacatggg cgcggtggcg gttggtgcga ctatctattt tggttcggaa gagtcacgtc
    13261 gccagattga agaaatttct gcggcttttg aacgtgcgca tgagctgggc atggtgacag
    13321 tgctgtgggc ctatttgcgt aactccgcct ttaagaaaga tggcgttgat taccatgttt
    13381 ccgccgacct gaccggtcag gcaaaccatc tggcggtaac catcggcgca gatatcgtca
    13441 aacaaaaaat ggcggaaaat aacggcggct ataaagcgat taattacggt tataccgacg
    13501 atcgcgtgta cagcaagtta accagcgaaa acccgattga tctggtgcgt tatcagttag
    13561 ctaactgcta tatgggccgg gctgggttga taaactccgg cggtgctgca ggcggtgaaa
    13621 ctgacctcag tgatgcagtg cgtactgcgg ttatcaacaa acgcgctggc ggaatggggc
    13681 tgattcttgg acgtaaggcg ttcaagaaat cgatggctga cggcgtgaaa ctgattaacg
    13741 ccgtgcagga cgtttatctc gatagcaaaa ttactattgc ctgatacgcc tcatacgaac
    13801 agcaccaccc cgtaggacgg ataaggcgtt tacactgcat ccggcaacct atgcctgatg
    13861 cgacgcggaa gcgtcttatc atgcctacat agcactgcca cgtatgttta caccgcatcc
    13921 ggcataaaaa cacgcgcact ttgctacggc ttccctatcg ggaggccgtt tttttgcctt
    13981 tcactcctcg aacaattttc atattgtcgt tttgtgatcg ttatctcgac atttaaaaac
    14041 aaatcatttc attatatttt gaaatcgaaa acaaacgaca ggatatgaaa atgtacgtgg
    14101 tatcgacaaa gcagatgctg aacaacgcac agcgcggcgg ttatgcggtt ccggcattca
    14161 atattcacaa tctcgaaacg atgcaagtgg tggtggaaac cgctgccaac ctgcatgcgc
    14221 cggtcatcat cgccggaacg cctggcacat tcacttatgc tggtacagaa aatctgttgg
    14281 cgctggtcag cgcgatggcg aagcaatatc accatccact ggcaattcat ctcgaccatc
    14341 acacgaaatt tgacgatatc gctcagaagg ttcgttctgg cgtgcgctca gtcatgattg
    14401 acgcctcgca tttgcctttt gcgcaaaata tttcacgggt caaagaggtg gtggattttt
    14461 gccatcgctt tgatgtcagc gtcgaagcgg agctggggca acttggcggc caggaagatg
    14521 atgtgcaagt caatgaagcc gatgcttttt ataccaaccc cgctcaggcg cgtgaatttg
    14581 ccgaggcaac cggaattgat tccctggcgg tcgccatcgg cacggctcat gggatgtatg
    14641 ccagcgcacc ggcgcttgat ttttctcgac tggagaacat tcgccagtgg gtgaacttac
    14701 cgctggtgct gcatggcgcg tcagggttat cgactaagga tattcagcaa accatcaaac
    14761 tggggatatg caaaatcaac gttgcaacgg agctgaaaaa tgccttctcg caggcgttaa
    14821 aaaattacct gaccgagcac cctgaagcga ccgatccccg ggattatttg cagtcggcta
    14881 aatccgcaat gcgcgatgtg gtgagcaaag tgattgccga ttgtggctgc gagggcaggg
    14941 cataacgcac ctgccattta acaaggaaaa aacatgaaaa cgttaattgc ccggcataaa
    15001 gctggtgaac atatcggcat atgttcagtc tgttctgccc atccgttggt tatcgaagcg
    15061 gcgctggcat ttgatcgcaa cagcacgcgc aaagtgctga ttgaagcaac gtcaaaccag
    15121 gtcaatcaat ttggcggtta taccggaatg acaccggcag actttcgcga atttgttttt
    15181 acgattgccg ataaagttgg gtttgcacgc gaacgcatta ttctcggcgg cgatcatctg
    15241 gggccaaact gctggcagca agaaaatgcg gatgcggcga tggaaaaatc cgtcgagctg
    15301 gtaaaggaat atgttcgtgc cggcttcagt aaaattcatc ttgatgcgtc aatgtcctgc
    15361 gcgggggatc ccataccgtt agcaccagaa acggttgcgg aacgagctgc tgtgttttgc
    15421 tttgctttgc tgcggaaagt gtggcgacag attgccagcg tgagcaactg agctatgtca
    15481 ttggcaccga agttccggtt ccgggcggtg aggccagcgc cattcagtca gtacacatca
    15541 cccatgttga agatgccgcc aatactttac gtacgtatca aaaggccttt attgcccgtg
    15601 ggctgacaga ggcgttaaca cgcgtgattg ccatcgtggt gcagccgggc gtggaatttg
    15661 atcacagcaa tattatccat tatcagccgc aggaagcgca ggcgctggcg caatggatag
    15721 aaaacacccg aatggtttat gaagcacatt ctaccgatta ccagacccgg acggcttatt
    15781 gggaattagt ccgcgatcac tttgcaatat tgaaagtcgg tcccgcatta acctttgctt
    15841 tacgcgaggc gatatttgca ctggcacaaa ttgagcagga acttatcgcc cccgaaaatc
    15901 gcagcggttg cctggcggta attgaagaag tgatgctcga cgaaccgcaa tactggaaaa
    15961 aatattatcg cacgggtttt aacgattcat tactggatat tcgttacagc ctgtcggatc
    16021 gtattcgtta ttactggccg catagtcgga ttaaaaatag cgttgaaacg atgatggtga
    16081 atctgcaagg cgtggacatc ccactgggca tgattagtca gtatcttccc aaacaatttg
    16141 aacgcattca gtccggggaa ttatcagcaa tgccgcatca gcttattatg gataaaattt
    16201 atgatgtttt gcgcgcctat cgctacggct gtgcggaata aggacggtat atgactaacc
    16261 tgtttgttcg tagcggaatt tcttttgtcg atcgtagcga agttttaacc catatcggta
    16321 atgagatgct cgcgaaaggt gtggtttatg atacctggcc acaggcatta attgccagag
    16381 aagcagaatt ccctaccggg ata
//
LOCUS       DAJOSY010000095        16214 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJOSY010000095 DAJOSY010000000
VERSION     DAJOSY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.095"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            118..660
                     /locus_tag="OHN27_003321"
                     /pseudo
     CDS             118..660
                     /locus_tag="OHN27_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Accessory colonization factor AcfD"
     gene            657..1226
                     /gene="pppA"
                     /locus_tag="OHN27_003322"
                     /pseudo
     CDS             657..1226
                     /gene="pppA"
                     /locus_tag="OHN27_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
     gene            1292..1702
                     /gene="gspS2"
                     /locus_tag="OHN27_003323"
     CDS             1292..1702
                     /gene="gspS2"
                     /locus_tag="OHN27_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HCQ2287705.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCNDPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            1720..2679
                     /gene="gspC"
                     /locus_tag="OHN27_003324"
     CDS             1720..2679
                     /gene="gspC"
                     /locus_tag="OHN27_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HCQ2287706.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIARGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEGHSTVAVTNKKAVSDEAKQAVAEPAASAPV
                     EIPTAVRQALTKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            2709..4769
                     /gene="gspD"
                     /locus_tag="OHN27_003325"
     CDS             2709..4769
                     /gene="gspD"
                     /locus_tag="OHN27_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HCQ2287707.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDKQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            4769..6232
                     /gene="gspE"
                     /locus_tag="OHN27_003326"
     CDS             4769..6232
                     /gene="gspE"
                     /locus_tag="OHN27_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HCQ2287708.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLQQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDNAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGSRAWGEYSGLDRSGGV"
     gene            6177..6359
                     /locus_tag="OHN27_003327"
                     /pseudo
     CDS             6177..6359
                     /locus_tag="OHN27_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
     gene            complement(6774..7100)
                     /locus_tag="OHN27_003328"
     CDS             complement(6774..7100)
                     /locus_tag="OHN27_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2287709.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(7097..7360)
                     /locus_tag="OHN27_003329"
     CDS             complement(7097..7360)
                     /locus_tag="OHN27_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HCQ2287710.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(7432..8298)
                     /locus_tag="OHN27_003330"
     CDS             complement(7432..8298)
                     /locus_tag="OHN27_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCQ2287711.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LTEISVLTESIGGKTARDWAMKQDFRCGCWLMDKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYENNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLYLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYSE
                     ALASTVNKSRKA"
     gene            complement(8383..8580)
                     /locus_tag="OHN27_003331"
     CDS             complement(8383..8580)
                     /locus_tag="OHN27_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287712.1"
                     /translation="MKSLTTETALDILIVWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IERAREDVRTLRHLQLLHQNR"
     gene            complement(8592..9080)
                     /locus_tag="OHN27_003332"
     CDS             complement(8592..9080)
                     /locus_tag="OHN27_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287713.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRASAERTPPSAALTGIRCSTAHITETDNAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            complement(9077..9454)
                     /locus_tag="OHN27_003333"
     CDS             complement(9077..9454)
                     /locus_tag="OHN27_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCQ2287714.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMARDNYRTVNNITLGKHPGAKQ"
     gene            complement(9501..9875)
                     /locus_tag="OHN27_003334"
     CDS             complement(9501..9875)
                     /locus_tag="OHN27_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCQ2287715.1"
                     /translation="MSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            complement(9955..10176)
                     /locus_tag="OHN27_003335"
     CDS             complement(9955..10176)
                     /locus_tag="OHN27_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCQ2287716.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10239..10715)
                     /locus_tag="OHN27_003336"
     CDS             complement(10239..10715)
                     /locus_tag="OHN27_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCQ2287717.1"
                     /translation="MQQLSFLPGAMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(10731..11210)
                     /locus_tag="OHN27_003337"
     CDS             complement(10731..11210)
                     /locus_tag="OHN27_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCQ2287718.1"
                     /translation="MKSVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNVMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(11292..12110)
                     /locus_tag="OHN27_003338"
     CDS             complement(11292..12110)
                     /locus_tag="OHN27_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2287719.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            complement(12200..12433)
                     /locus_tag="OHN27_003339"
     CDS             complement(12200..12433)
                     /locus_tag="OHN27_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCQ2287720.1"
                     /translation="MSDCHPVLLPEGPFSREQAVAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNSYLASEGILRQ"
     gene            complement(12439..13116)
                     /locus_tag="OHN27_003340"
     CDS             complement(12439..13116)
                     /locus_tag="OHN27_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287721.1"
                     /translation="MNTRALFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            complement(13264..13944)
                     /locus_tag="OHN27_003341"
     CDS             complement(13264..13944)
                     /locus_tag="OHN27_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCQ2287722.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMAGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRYSLRRQSSPGAIPEMLSFIQNTGIARILPLRNGRLITCL
                     TDNQEPSPCLIWLPAPDITATFPECFSQLILAIRQCIHISLMTERWYPSLEPCRLIYY
                     SGSWYLIALQKGKLQVFPLADIKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIH
                     KLINTFNL"
     gene            complement(14147..15031)
                     /locus_tag="OHN27_003342"
     CDS             complement(14147..15031)
                     /locus_tag="OHN27_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCQ2287723.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDMPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRQQELSLATVTARVATLFPSSFPVLSVAAPVGWNLPALVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGDAIGKSFDAAVAQFSFPAWMLQLLR
                     KARDRIIHLLVTLWDRLF"
     gene            15216..>16214
                     /locus_tag="OHN27_003343"
     CDS             15216..>16214
                     /locus_tag="OHN27_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287724.1"
                     /translation="MTGRKNKCVRKNCNNQVFSVKMAVEKRLCRKHYERFCELKKRRE
                     QNPVHRLNEALPRKIKGPKSKKKLVKEREIINSRKKHKQEQGPSLAELNKKVREILKT
                     PAWRTLETRIRRNGITFKSKAELDVLSELYAIYSWVSYCKRSDNYSVWFLNCPDVNIN
                     LHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYSANSSGVSGHNDSKSLYKTVLSVC
                     GGKRETELFFYSLPVKTNHHPYWIKSGVVKRFPPVFGLLFYEQERLTGGVFRYVLIRF
                     YNTFKENLFFVEIVSCCFLVELFHDIDSFFINQRLSKLESFIDKWREGKEIWIKAYL"
BASE COUNT         4016 a         3885 c         4327 g         3986 t
ORIGIN      
        1 tttctatcaa tgatgtcgtt ttcttaagaa tggaggaagt tattagagaa gataaagcat
       61 aaaactattt ctcccagtta cgaatttttt aacattgttt tgtcacttgc gttattaatg
      121 aataagaaat ttaaatataa gaaatcgctt ttagcggcta ttttgagcgc aaccctgtta
      181 gccggttgtg atggcggtgg ttccggatct tcctccgata cgccgcctgt agattctgga
      241 acagggtctt tgccggaagt gaaacctgat ccaacaccaa acccggagcc gacgcctgag
      301 ccaacgccgg acccagagcc tacgccagaa ccgatacctg atcctgaacc aacaccagaa
      361 ccggagccag aacctgttcc tacgaaaacg ggttatctga ccctgggcgg aagccagcgg
      421 gtaactggtg ctacctgtaa tggtgaatcc agcgatggct ttacatttaa acctggcgag
      481 gacgttactt gcgtggcggg taacacgaca attgccacct tcaacactca gtcagaagct
      541 gcgcgtagct tgcgtgcggt tgaaaaagtg tcgtttagcc ttgaggacgc gcaagaactg
      601 gcgggctccg atgacaagaa aagcaatgcg gtttcgctgg taacgtccag taacagctgt
      661 tctcctggct gatgctcaaa gggcgctgcc gcgactgtca ggcgaaaatc agcaagcgtt
      721 atccgctggt ggagttattg acggcactcg cttttttgct ggcgagtctg gtctggccgg
      781 aaagtggatg ggcgctggcg gtgatgatat tatccgcctg gctgattgcc gcgagcgtca
      841 ttgacctcga tcaccaatgg ctgcccgatg tttttactca gggcgtattg tggacgggac
      901 tgagtgcggc atgggcgcag cagagcccgc tcacgctaca agatgcagtt accggcgtcc
      961 tggtggggtt tatcgctttt tactccctgc gctggatagc cggaatagtt ctgcgtaaag
     1021 aagcattagg catgggcgat gtattattgt tcgctgcgtt aggtagttgg gtggggccgt
     1081 tgtcgctacc caatgttgct ttaatcgcat catgctgcgg cctgatatat gccgttatta
     1141 caaaaagagg atcaaccaca ctgccttttg gaccgtgttt aagtctgggc ggtatagcaa
     1201 caatttatct acaggcattg ttttaatgat aacaacgtca ttatcaaagt gacattttaa
     1261 ctcttattaa taaccttaga gattatttac catgtcgata aaacaaatgc cagggagggt
     1321 attaatatcg ctattgttga gcgttacagg attattaagt ggctgtgcca gccataatga
     1381 aaatgccagt ttactggcga aaaaacaggc gcaaaatatc agccaaaacc tgccgattaa
     1441 atctgcggga tataccttag tgctggcaca aagtagcggc acgacggtaa aaatgaccat
     1501 tatcagcgaa gcggggactc agaccacgca gacgcctgac gcctttttaa ccagctatca
     1561 acgacaaatg tgcaatgacc caacggtgaa attaatgctc actgagggaa ttaattacag
     1621 cataacgatt aatgatacac gtacaggtaa ccagtatcag cggaaactgg atcgtaccac
     1681 ctgtggaata gtcaaagcat aacgtcgggt agatataaat tggcgcgggt tgtttttcgt
     1741 gacgcacgaa tttatctcat tcaatggctg acaaaaattc gtcacactct taaccagaga
     1801 caatctctta atacagacaa agagcatctg cgcaaaattg cacgcgggat gttctggctg
     1861 atgctgctta ttatttctgc aaaaatggcg tattcactct ggcgctattt ctccttttct
     1921 gcggaatata cggctgtttc cccatcggcg aataaaccgc cccgtgcgga tgcaaaaacg
     1981 ttcgataaaa atgacgtgca attaatcagc cagcaaaact ggtttggcaa atatcagccc
     2041 gtcgccacgc cggtaaaaca acccgaacct gtgcctgtgg cagaaacgcg tcttaatgtg
     2101 gtgttgcgtg ggatcgcctt tggtgccaga cccggcgcgg ttattgaaga aggtggcaaa
     2161 cagcaggtct atttgcaggg ggaacggctt ggttctcaca acgcggtgat tgaggaaatc
     2221 aaccgcgacc atgtgatgct gcgctatcag ggaaaaatag agcgcctgag cctggcagaa
     2281 gaggggcatt ccaccgtagc cgtgaccaac aaaaaagccg tcagtgacga agcaaagcaa
     2341 gctgttgctg agcctgctgc cagtgcgcca gttgagatcc cgactgccgt gcgtcaggca
     2401 ctgacgaaag atccgcagaa aatttttaac tatatccagc ttacgcctgt gcgtaaggag
     2461 gggattgtcg gttatgcagt gaagccgggg gcagatcgtt ctctgttcga tgccagcggt
     2521 ttcaaggaag gcgatatcgc cattgcgcta aatcagcagg atttcactga tccacgagca
     2581 atgattgctc tgatgcggca gttaccttca atggattcca ttcaacttac ggttttacgc
     2641 aagggtgcgc gccacgacat ttccatcgcg ctgcgctaac cgcatttaat ccaggagaat
     2701 cattcatcgt gttttggcgt gatattacgt tgtcggtctg gcgtaagaag acaactggcc
     2761 tcaaaacaaa aaagcgttta ctgccgctgg tgctggcagc ggcattatgc agttcaccgg
     2821 tctgggcgga agaagccact ttcaccgcta attttaaaga taccgacctg aaatcgttca
     2881 tcgaaaccgt cggcgctaac cttaataaaa ccatcattat ggggccgggc gtacagggga
     2941 aagtgagtat tcgcaccatg accccgctca atgaacgcca gtattaccag ctattcctta
     3001 acctgctgga agcacagggg tatgccgtcg taccgatgga aaacgacgtg ctgaaggtgg
     3061 tgaaatccag cgctgcgaaa gtcgagccgc tgccgctggt cggtgaaggc agcgacaact
     3121 acgcgggcga tgaaatggtc accaaagtag tgccggtacg taatgtgtca gtacgcgagc
     3181 tggcaccgat tctgcgccag atgatcgaca gcgcaggctc aggcaacgtt gttaattacg
     3241 atccctccaa cgtgattatg ctcaccggac gcgcctccgt cgtggagcgg ctgacggaag
     3301 tgatccagcg tgtggatcac gcgggtaatc gcactgaaga ggtgatcccg ctggataacg
     3361 cctcggcttc ggaaatcgcc cgcgtgctgg aaagcctgac gaaaaacagc ggcgagaacc
     3421 agcctgcgac gctgaaatct caaattgtcg ccgatgaacg caccaacagt gtgattgtca
     3481 gtggtgaccc cgccacgcgg gacaaaatgc gccgtctgat ccgtcgcctg gactcagaaa
     3541 tggagcgcag cggcaacagc caggttttct atctcaaata cagcaaagcc gaagatctgg
     3601 tcgatgtact gaagcaggtc agcggcacgc tcacggcggc taaagaagag gcggaaggca
     3661 cggtcggtag cgggcgtgag attgtctcca tcgccgccag caaacacagt aatgccctga
     3721 ttgttactgc gccgcaggac atcatgcaat cgctgcaaag cgtgattgaa caactggata
     3781 ttcgccgtgc tcaggtgcat gtcgaggcgt tgatcgtgga agttgccgaa ggtagcaata
     3841 tcaatttcgg cgtgcagtgg gcgtcgaaag atgccggatt aatgcagttt gctaatggta
     3901 cgcagatccc tatcggtacg ctgggcgcag ccatttctca ggcgaaaccg cagaaaggct
     3961 cgacggtgat cagtgaaaac ggcgctacca ccattaatcc ggataccaac ggcgatctct
     4021 ccacgctcgc ccagcttctt tccggcttta gcggtacggc ggttggtgtg gtgaaaggcg
     4081 actggatggc gctggtgcag gcggttaaaa acgactccag ctctaacgtg ctctccacgc
     4141 cgagcatcac cacgctggac aaccaggaag ccttcttcat ggtgggccag gacgttccgg
     4201 tattaaccgg ctctaccgtt ggctccaata acagcaatcc tttcaataca gtagaaagga
     4261 aaaaagtcgg catcatgctg aaagtcacgc cgcagattaa cgaaggtaac gcggtacaga
     4321 tggtgattga gcaggaagtc tcgaaggtgg aaggacagac cagcctcgac gtggtgtttg
     4381 gtgagcgcaa actgaaaacc accgtgctgg ctaacgatgg tgagctgatt gtgcttggtg
     4441 gtctgatgga cgatcaggcg ggagaaagcg tggcgaaagt gccgctgctg ggcgatatcc
     4501 cattgattgg taacctgttt aaatcgacgg cggataaaaa agaaaaacgt aacctgatgg
     4561 tgtttatccg tccgaccatt ctgcgtgacg gtatggcggc agacggcgtg tcgcagcgca
     4621 aatataacta tatgcgcgcc gaacagatct atcgtgataa gcaaggttta agcctgatgc
     4681 cgcacactgc acagccggta ctgccagcgc aaaaccaggc cttaccgccg gaagttcgcg
     4741 cgttcctcaa tgccgggaga acgcgttaat ggtgcctgta gcacaggaaa ccaccgccaa
     4801 caccgtgcgt ctgccctaca gtttcagccg tcggtttagc ctggtggcat ggtgcgaagc
     4861 gtcgctggag atcctccacg ttcatccgct atcgctctct gttttgcagg agctgcagcg
     4921 ggggctgaac gcgcccttta cgctgcagca aatcgacgag gccgaatttg aacagcggct
     4981 gaatgcggtc tggcagcggg actcttccga ggctcgccag ctgatggaag atctcggttc
     5041 tgccgaggac ttttttaccc tcgcggaaga actgccggaa acggaagatc tgctggaaag
     5101 cgacgacaat gcgccgatca tcaaactgat caacgccatg ctggcagagg cgattaaaga
     5161 aggcgcttcg gatatccaca tcgagacgtt tgaaaagagt ctggtgatcc gttttcgcgt
     5221 tgacggcaca ttacatgaaa tgctgcgccc ggggcgcaaa ctggcctcgc tgctggtatc
     5281 gcgtatcaag gtgatggcac ggctggatat cgccgaaaag cgcgtgccgc aggatggacg
     5341 tattgcgctg ttgctgggcg gccgggcgat tgacgtgcgt gtctccacca tgccttctgc
     5401 ctggggcgag cgcgtggtgc tgcgactgct ggacaaaaac caggcccgcc tgacgctgga
     5461 gcgtctgggg cttagccagc aactgaccgc gcagttgcgc cagctgttac acaaaccgca
     5521 cggcatcttt ctggtgacgg ggccgacggg ttccggcaaa agcaccacgc tgtacgccgg
     5581 attgcaggag ctgaacaacc attcgcgtaa cattctcacg gttgaagacc ccatcgaata
     5641 catgattgaa gggatcggtc agacgcaggt taacacccgc gtcggcatga ccttcgcccg
     5701 tggcctgcgc gcgattttgc gtcaggaccc ggatgtggtg atggtcggtg aaatccgcga
     5761 taccgaaacc gcagaaattg ctgtccaggc atcgcttaca gggcacctgg tcctttccac
     5821 gctgcatacc aacacggcgg tgggggcgat cacgcgtttg caggatatgg gcgtggagcc
     5881 tttcctgctc tcttccagtc tgacaggtgt gatggcgcag cggctggttc ggacgctgtg
     5941 tcccgactgc cgccagcccg cgcctgccac tgacgaagaa aaacgcctgc tgggaattac
     6001 cgacgcccgt actgtcactc tgtaccatcc gcagggctgc cccgcctgta atcacaaagg
     6061 ttttcgcgga cgtactgcca tccatgagct gatcgtggtg gatgccacat tgcgtgattt
     6121 gatccaccgt caggccgggg aactggagct ggaacgttat gtccggcaac actctgcggg
     6181 tagccgagcg tggggagaat attcaggtct ggatagatcc ggtggtgttt aatgacctgc
     6241 tgaactggtt aaacgcactg gatgaaaaat atgcgctgcg ggtgacacaa attgatgtca
     6301 gtgctgctga gaagcctggg atggtgaatg tgcagcggct ggagtttgga cgggggtaaa
     6361 gtatcaggag attaatatca ataaaattac tctcgttaac gtggcgagag taattccccc
     6421 agactttata agctaatttt tttcgtgaag gttattgtca gatccaaaag caaaaacccg
     6481 ccttgtgggc gggttcttaa gaattagtgg tgcccggact cggaatgaaa ctggttatat
     6541 aaccaattgt tttgtatcga ctgttttatc cttgttcttt ttggggccac caatgaggcc
     6601 cccatgtgca attcgaattc ttttgacgct ataatccctc ttttttgtgc tttgttgtaa
     6661 tcattttgtc tggttcgata tgctgaatcg cggaatcgaa gtaacaacaa aatacagaga
     6721 tttataaaat acctggctac ctgtgttagg gagcgttaac gcacagattt tgtttatggc
     6781 tgccagaaat gatctgatgg tttgtcgtct ggctctgcaa gtaccctgtt gatatcccgg
     6841 gaaccatgaa cgatacgttc gatacgaact tcagtatcca gtaccctgaa tagcagaagg
     6901 taacgaccat acgaacaacc tctgatccct gcccctaact cgggtctttc tctgtagagt
     6961 actggcgatt cggctatcaa cagacattgt tggtaaagct cttcggtaaa actgattgca
     7021 cggacaggat tatcctgagc gatccagtca cctatagctt caagatcgtg ttctgctaat
     7081 ggtgaaacag tcagtttcat attggctgtt caccttctgc catctttttg tatttgtgcg
     7141 tcagtcgccc gaatacctct tcagcacttt tactttcacc gctgttgatg cctgcggtaa
     7201 cggcgctctg gagtgattca agctttatct gctgttcccg ttcttccaga gcacgaagtc
     7261 cggcacgaat gacttcactt gtattgttat accgaccact ttctatttgt tcgcggataa
     7321 acgtttcaaa gtaaggactg agggatacac tggtaggcat ggtgattttc tcgccgattg
     7381 attactatca attaataata gttaatattc gcgaattgta aaatacctct tttatgcctt
     7441 gcggcttttg ttcactgtgc ttgccagggc ttccgagtag tgtctggcaa taatatcatt
     7501 aagcctgtca accagttctg gtttcctgaa cgtgatgtgc gcggagccct tcttaaagta
     7561 tctgatgctg aacatctcat cttcatagtc ctctttaccc tgaacagact ggatgtggtc
     7621 atccagatga atggtgatgt tttcccggtt gtcggggatc ggtttgccgc tgaacagata
     7681 cagcattctt tccaggtcgg caagtcgatc tgcctgttgc ccggtgataa gatgaaaccc
     7741 ccaccggtcc cacctcacca ggttgttgac gataatttta ctgccaaatt tacagggtga
     7801 attgttttcg taattccagc tcagtcccct gaaaacattg atcaccccac gctcaaacac
     7861 ttcatctttg ttctggtgca gttgttcaaa tgtgctcaga atgttcgctt cactgatttc
     7921 cggaaaatta tcgtattcca gtgaccggta ccatgtatca cgggcctgcg catccattaa
     7981 tgacagcatc ccggaacgtt gcatcaggtc acgccagatt tcgcgatcga gattgcgggt
     8041 gatggctttc attgctgttt ccggtttatc catcagccag cagccacagc gaaaatcctg
     8101 tttcatggcc cagtcccggg cggttttacc tccaatgctt tccgtcagaa ctgagatttc
     8161 agtcagttgc cggataagtg tgtcgatttg cagcagtgta ctctttcgta cagccagaat
     8221 gtggcggata ctggttgagc agataacgtc cgtatgcccg ttgaccactt ccggacggga
     8281 ggctgtgata tcagacataa tcgatattcc tttttgcaga taaatgaaaa cgccagccct
     8341 gttcaggctg gcgtcagtgg aatgaagtct ggtgagtaat atttaccggt tctggtgcag
     8401 aagctgcaga tgacgaagag tacggacatc ctcccgggcc cgttcgatac agggcaacag
     8461 tgctgccgaa tccgttttat cctcatcgtt gtcaaagata attcccgatt cgcagtcgat
     8521 attgtcctgc agccacacaa tcagaatatc cagtgctgtt tccgtggtta atgatttcat
     8581 gttgttcctg gttaccagtc gaaagtgggc aggtcaggca ggcattcagc gctggcatcc
     8641 agatgaatga gactgacgcc ataacgccgg ataagtgtga caaccagacg gcggaacgtt
     8701 tttgacagtc ccaggcgttt aagccgcaga accgggtatg accacgcatc ggtacgtaac
     8761 agatatccgc tacctgtaaa atgaatccat tctgattcgc cgaagtcatt cgtctgatgt
     8821 gacagcgagt acaaccaggc attgtccgtt tccgtgatat gtgcggtact gcagcgtatg
     8881 ccggtaaggg ccgcagatgg tggcgtgcgc tctgctgatg cccggtcaga ttcatcaaca
     8941 acacgaaggg agtaaccgtt atcgcagacc acgttaatca gttcagcgag agtaacacca
     9001 tcgacgtcaa cgacaatgcg ccccatgttc agtgcctgca cgttaatgct gtcggcttcc
     9061 agcgtcaggg ccagtttcat tgtttcgccc ccggatgttt acccagggta atgttattta
     9121 ccgttctgta attgtcgcgg gccatcaggc cggttgcccg gcgggcccgg aggatatcaa
     9181 tactgtttat taactgagaa cgggtacagg cgctgaatcc cggctggtcg gtacgtacca
     9241 gtgcgtattt ttcaacgaga aagttcaccg catcgcacag tgaaatgcct gcctcaatat
     9301 gctgctcaat cacacgttca tcagcgaacg gtgtgtcatt cagtgtgagg ccgtaatgct
     9361 ggtccaacag tcgggtgagc agtgtctgcc agatggtgac gggagacggg cagcgcgatg
     9421 cctcccgtac gtgtgtgtca ggtaatgttt tcatcttaag ttctctgtaa ctggtagtgc
     9481 taaaggtgag atattcgggg ttatgaggtg gttgcgggtg ccagtgtcgg ataaacagcc
     9541 atataaacgt aaccacagct gccgagggtg tcggcttcgc aggtcagccc tctggcatac
     9601 agcgtgacgg tatgctgatg gcggggattc agttcaccgc tggtgagcat gagttccagt
     9661 tgtttcatca gcagcggaaa ggcctggtcc agatgatacg catccgcgtc gctaaactgc
     9721 cccctgatac cggcgcggtc ggcaaggtaa tgcaactgat tgccctcctg caccagacgt
     9781 gccccgaaac agggcgtcac ggtgcagggg agcccccacc aggggcggtc gttgttgttg
     9841 tcgggatgcg ttgtcccggg gtgtgtgtct gacacgataa aatcctcaca gaaaatcggt
     9901 taagaatgct ccgatggtgg cgataattcg gctcatcaga atgccctttc tgattcagtt
     9961 cagggtaacg ctcatcagac ggacatacgg accaaaactg tccttacggc gttcagcaaa
    10021 cacggccagc acaccgggaa tatcctgcac ttcacggccg gtatacgtat ccgtgctacc
    10081 gtgccagcgg tatttaccgg tacagaacgg aaaaagacgg gatgcaggat gctgttggtg
    10141 aatacgcatg gcttcaccac gggtgatgat tttcataatg ggatacctct gaagacagaa
    10201 gataaaagtg aaaacaggtg tgatgtggtt gtgacgggtt aaagcagacc gtgttcggca
    10261 aaggagaata cctggttgcc accgactatc agatggtccg gcacccggat atccaccagg
    10321 gccagcgcct gtaccagacg ttcagtgata agccggtctg ccttactggg ggtgacttca
    10381 ccggacgggt gattgtgcgc cagcaccacg gcagcggcat tgtggtacag ggcacgttta
    10441 atcacctccc ggggatggac ttctgtgcgg ttgatggtgc ctgtgaagag ggtttcaccc
    10501 gcaatcagct gattctggtt gttcagatac agcacccgga actcttcccg ctccagtcct
    10561 gccatgttca gaatcagcca ttcccgtgcc gcatgcgtgg aggtgaaggc cacgccgggc
    10621 tcatgaagat gacggtccag ggttttcagg gcccgctgaa tgaggctgcg ctcgccgggc
    10681 gtcatcgctc cgggcagaaa ggaaagctgt tgcattatgc ttctctccat tcagtcgatg
    10741 atgcgcataa tggcgctgca ttccggatgc tgcagggcgt aatcccgcaa ccgataataa
    10801 tggaccgtca tggcataatt ctccgtacga caggcgtgat gactgtacgt catcagacag
    10861 gcggcaatgc cggcggcttc cgggctcatt tcagcgcggt taccgttcat gacgttaaac
    10921 agcacccatg tttcgtcatc atcgtcatcc ggttcgggtg ccataaatgc gccaccgttg
    10981 ttcagggtgt acagattcca gataccaccg cagtagtctt cacacagacg gtccatccag
    11041 ccgaagacac ggggctccag ggtcacccac tgtggaatga ggccaaaatg ctgcggccaa
    11101 aagctgatgc gctgttcatc agggacgagg ctggcaacca actgaggcgg attattctct
    11161 ggtgttgtag ctgaataaat tgtgggggta ttctgagaaa cgcttttcat gagataactc
    11221 cttaaataac aaataaaagt gtgtttttca ggaacgaaag ctgaatgatt ccctcactca
    11281 gggaaacggc atcagcgcag gctctccagc atcgtttctg ccatcaccca caatgcgcgg
    11341 ttgagtttaa tatcggtgtc gatgctgtga atggcccggg tatggatacg ttttcctttt
    11401 gcactgcgac cggaaatccc gcctttcagc atattctcct ggatggtctg atatgcgctc
    11461 cacaggtcct taccgtaatc ctcccggcgt cgcggcgtca gaatgtcggc ggtggtgacg
    11521 ggctgatgtt cgtcaccata acggtaagtc agtgccgcct gtgccagcgc ctggcgtgcc
    11581 ggtggcggca ggaccagcga ctgcatggca tcacgcttct cctctatccg gtcaaacacg
    11641 cccaccactt cgtaagcccc ttcaatgact ctgtccacca catttccccg gtgcggaaca
    11701 cgcacttccc ccagagactg gccacagacg cacccgttct ggcagacgaa cctgaagtaa
    11761 cccggcagca tctggtagct ggaggtaccg tcatgagagt tgagcagaat aatttcaggg
    11821 acatgttctc cgtttatctc tccggcccgc cgcagacgca gcatgtgttt ggtatattcc
    11881 cggcggctct ggtcgcgcac acgggtctgg caggcgaaga acggctgaaa gccttcccgc
    11941 tgcagatttt ccaggacggt aatggtggga atgtacgcat accgttcact gcgggaggtg
    12001 tgccggtctt ctccaaaaat actcgggacg tggtacatca gttcttcgcg tgtcagtggg
    12061 cgctcccgac ggatctgatt tacatgacca aaacgactgg ctaattgcat actttgctcc
    12121 ttatgaatga attagcggta taaacgaaaa agccgcgtct cccggagaag acgcggcctg
    12181 atggatgaaa tgaagacgtt tattgcctga gaattccctc actggcgaga tacgaattta
    12241 gctgttttcc ggcatcaggt tcaaaattcc agcaccgcca gcgtagctgc ccttcggcat
    12301 tgcggataac cagccggaaa tgcgttccct ggtcatcttc aataagcaca ttgcggtaag
    12361 ctgttgtgac ggcaaccgcc tgttcgcgtg agaaagggcc ttcgggcaat aaaacagggt
    12421 ggcagtccga cataccgact attttccgat aaattcaacg ctggcgctgg tggcattacc
    12481 gtcagcacca accgtgacgg tcactttgtc gtacgcacgg tcttcaggat tgaatacttc
    12541 aaactcaacc ttattgcctt ttacttcgta agagccaaaa tcagcggttt ctgttgtttc
    12601 ctcgtcatat tcccgggcgt atgcctgccg ggcagcctgc aggacggaat ctttattaat
    12661 agtggtcgtc tgtttcgggg aaaaaccggc aagtgatgtg acaaatttcg gacagacctt
    12721 cagcatatca ccgcgggtga attccattcg tacaccaaat gcctgtaaaa cggacacatc
    12781 acgctctgct atggagtgaa cggctttttt gaccatttcc ggcgcctgtc cgatttccac
    12841 ctcaacagcc ctgaatcccg tctgctgatt acacatttgt gatgtgggga tcaggttacc
    12901 gttaatactg atacggctgc ccaggagcgt ctgtacacca ttcgggcatc cctgcatcag
    12961 gccagtaata ccaatcgtgc catttttcag cattaccagc gcctgaccat cttccgtcat
    13021 ggtctgacac cagccgtttt tgacagccca gttgccggcg gaggcggaga atgatgccac
    13081 agtgaacagc aggggaaaaa gagctctggt attcatgact gacctctgaa atgaacgtac
    13141 ggtgttattg ataatgatgg aaggtatgca gtgactgttc agggaatgac gtagccgtat
    13201 atttagtttt ctgttcaaca tattgtctcc atacctgtcg ggacggcttt ggcaggccgg
    13261 cgatcacagg ttaaaggtgt tgataagttt atggatgaaa gagaaatgtg gcagggcgga
    13321 gataaaacgc tcttcagcga ccagactgtg gatgtggcct ctccgttcaa accgttctga
    13381 tgtcaggctg actgatttga tatctgccag aggaaagacc tgcagttttc ccttctgtaa
    13441 cgcgatcaga taccagctac cgctgtaata aatgagccgg cagggctcca gtgacggata
    13501 ccatcgctca gtcatcagag agatgtggat acactgtctt attgccagga tgagttgcga
    13561 gaaacactcg gggaacgttg cagtgatatc cggcgccggt agccagataa ggcagggaga
    13621 gggctcctgg ttgtcggtaa gacaggttat cagtcgtccg ttccggagcg gaagtatccg
    13681 tgcgatcccg gtattctgta taaaagaaag catttcaggg atcgcacctg ggctgctctg
    13741 tcgtctgaga ctgtaccggc catttctgta ctctaagtcc aggtgaacca ggcgctgatg
    13801 aaaatcgcgc tgtaaggtac gttctgtaac accaaattca tctgatagtg tttttagtga
    13861 cagagattct ccggccatca gtcggctgat aatgagtgat aagcgaaccg ccaggcgatc
    13921 atgccgggaa cgattatcag acatagatgt gcactccgta agaaaacatc ttttatttca
    13981 tgatgttatt atgcatcatg atgttgaaag gatgtgtcgc atccgaataa tcagaaataa
    14041 aatgcagcag aaaaaatatt cctttaatta tctgcttttt aagcagggtt tcataaaaag
    14101 aagaatgctt gttaatccag cgacacatcc tgtcggcgtg agtgtgtcag aacagacgat
    14161 cccacagtgt gaccagcaag tggataatgc ggtcccgggc cttacgcaga agctgtaaca
    14221 tccaggccgg aaagctgaat tgggcaacgg cagcgtcaaa gcttttcccg atggcgtcac
    14281 caaaagtttg ttgtgcgtgt ttccgggcct gttcagagcg gttttccccc cggatatgtg
    14341 aataaactgc gctggttgcc tgtggtggca gtgcgtggat catcagcgac accagtgccg
    14401 gaagattcca gccgacaggt gcggcaacgg agagtaccgg aaatgatgac gggaacaggg
    14461 ttgccacccg ggctgttact gtcgccagtg agagttcctg ctgacgggac gggcattttt
    14521 ctgtgtcgtt ccattcttca gcaggaaaca cgcgatcggc atggctgaga acaaacagaa
    14581 aacgcgaggg atctgcacct tcattcagta aaaactgatg catggcaata tcagcagcat
    14641 acgcacgttc atcagcccgc aggatccaga taatcagatc cagttccggc agtaactgac
    14701 ggtaaagctc ccggtattcc tgatcatgct gcggtgtctc accaatgccg ggcatatcga
    14761 ccagcgtcat tctgcgttca ccgagctgca gggtaagacg atgagcctgg cgggtgcagc
    14821 cgttcagggg atgcgtggcg cagatacggg actgaaaaat ggcattacac agactgcttt
    14881 tgccggtgcc ggatttaccc ataatgccaa ttacgggctc gtaatgaata acaccgtgga
    14941 gatgttcaag gattttctgg cgaatttctt caggaagaaa cgtcagatat gattttactt
    15001 gtgcagagag ggaagaatgg ctgaatgaca taccggtatc ctgttgattt cgctgaaccg
    15061 gatcacacta atccggttta tcattcaggt aatatggacg tgaaaaatga tggaatgtct
    15121 gaattacgga catgattgtt aaagtgtaat ggagtatgaa gtgagttttt ccgaacggga
    15181 actaatgaca tactgcggtt tcaggagatg agaggatgac cggacgtaaa aacaaatgtg
    15241 tcagaaaaaa ttgtaacaat caggttttct cagtaaaaat ggcagtggag aaaagactgt
    15301 gcagaaaaca ttatgagagg ttttgtgagc tcaaaaaaag aagagagcaa aatcctgttc
    15361 acagattaaa tgaagcattg ccaagaaaga taaaaggacc aaaaagtaag aaaaaattag
    15421 taaaagaaag agaaatcatt aacagcagaa aaaaacataa acaggagcag ggaccatcgc
    15481 tggctgaact taataaaaaa gttcgtgaga tactgaaaac tccggcatgg agaacgctgg
    15541 aaacgagaat tcgaagaaac ggtatcacat ttaaatcaaa agcagagtta gatgtacttt
    15601 ctgaacttta tgcaatttat tcatgggttt catattgcaa acggagcgat aattattctg
    15661 tgtggttttt gaattgtccg gatgtaaata taaatcttca tgtgtgcgaa tgccggtctt
    15721 ctgaaagcga ttgttatctg ccatcccgtt ttattattct tccagctcca ttaaggcaaa
    15781 aagtaaaaag aaaatactct gctaattcgt ctggggtatc aggacataat gattcaaaaa
    15841 gtctttataa gacagttttg tctgtttgtg gtgggaaaag agaaaccgaa ttgttctttt
    15901 attcactgcc agtaaagact aatcatcatc cttactggat taaaagtggt gtggttaaaa
    15961 gatttcctcc tgtttttggt ttgttgtttt atgaacagga aagattaaca ggaggtgttt
    16021 ttcgttatgt gttgataagg ttttataata cttttaagga gaatttgttt tttgttgaga
    16081 ttgtctcgtg ctgttttctt gttgagcttt ttcatgatat tgattcgttt tttataaatc
    16141 aacgattaag taagctggaa agttttatag ataagtggag ggagggtaaa gaaatctgga
    16201 taaaagcata tcta
//
LOCUS       DAJOSY010000096        15514 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJOSY010000096 DAJOSY010000000
VERSION     DAJOSY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.096"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            492..2123
                     /gene="oppA"
                     /locus_tag="OHN27_003344"
     CDS             492..2123
                     /gene="oppA"
                     /locus_tag="OHN27_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCQ2287725.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2209..3129
                     /gene="oppB"
                     /locus_tag="OHN27_003345"
     CDS             2209..3129
                     /gene="oppB"
                     /locus_tag="OHN27_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCQ2287726.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3144..4052
                     /gene="oppC"
                     /locus_tag="OHN27_003346"
     CDS             3144..4052
                     /gene="oppC"
                     /locus_tag="OHN27_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCQ2287727.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4064..5077
                     /gene="oppD"
                     /locus_tag="OHN27_003347"
     CDS             4064..5077
                     /gene="oppD"
                     /locus_tag="OHN27_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCQ2287728.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5074..6078
                     /gene="oppF"
                     /locus_tag="OHN27_003348"
     CDS             5074..6078
                     /gene="oppF"
                     /locus_tag="OHN27_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCQ2287729.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6131..6460)
                     /locus_tag="OHN27_003349"
     CDS             complement(6131..6460)
                     /locus_tag="OHN27_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCQ2287730.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6495..7955)
                     /gene="cls"
                     /locus_tag="OHN27_003350"
     CDS             complement(6495..7955)
                     /gene="cls"
                     /locus_tag="OHN27_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCQ2287731.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8098..8271
                     /locus_tag="OHN27_003351"
     CDS             8098..8271
                     /locus_tag="OHN27_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCQ2287732.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8326..9579)
                     /gene="kch"
                     /locus_tag="OHN27_003352"
     CDS             complement(8326..9579)
                     /gene="kch"
                     /locus_tag="OHN27_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCQ2287733.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9879..10175)
                     /locus_tag="OHN27_003353"
     CDS             complement(9879..10175)
                     /locus_tag="OHN27_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCQ2287734.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10399..11118
                     /gene="tonB"
                     /locus_tag="OHN27_003354"
     CDS             10399..11118
                     /gene="tonB"
                     /locus_tag="OHN27_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCQ2287735.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11158..11556)
                     /gene="yciA"
                     /locus_tag="OHN27_003355"
     CDS             complement(11158..11556)
                     /gene="yciA"
                     /locus_tag="OHN27_003355"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCQ2287736.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11661..12200)
                     /locus_tag="OHN27_003356"
     CDS             complement(11661..12200)
                     /locus_tag="OHN27_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCQ2287737.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12230..12973)
                     /locus_tag="OHN27_003357"
     CDS             complement(12230..12973)
                     /locus_tag="OHN27_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCQ2287738.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(13001..13090)
                     /locus_tag="OHN27_003358"
                     /pseudo
     CDS             complement(13001..13090)
                     /locus_tag="OHN27_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13330..13968
                     /gene="ompW"
                     /locus_tag="OHN27_003359"
     CDS             13330..13968
                     /gene="ompW"
                     /locus_tag="OHN27_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCQ2287739.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(14028..14534)
                     /locus_tag="OHN27_003360"
     CDS             complement(14028..14534)
                     /locus_tag="OHN27_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCQ2287740.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQNIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(14580..15080)
                     /locus_tag="OHN27_003361"
     CDS             complement(14580..15080)
                     /locus_tag="OHN27_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCQ2287741.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLAINNVNK
                     KAENKA"
     gene            complement(15166..15345)
                     /locus_tag="OHN27_003362"
     CDS             complement(15166..15345)
                     /locus_tag="OHN27_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCQ2287742.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         4134 a         3620 c         3746 g         4014 t
ORIGIN      
        1 gtgctaaata ataatcaatt gttaaattat tgtgcatttc actactggaa ctgtaatcag
       61 aaaagataga catgcttagc caatctctat ttgattgaat tgaaagatgt ttgttaaggc
      121 atggatgcaa gctatagatt ctgatacggt caataaaaga gaattgctta acaattttgc
      181 aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc gggcaaccgc cagacgattc
      241 tttattggta atgagaataa ttaacaatta aagagcgtcg cgaaagaata atgtgtctcg
      301 acaggggaga cacagtacga atcgacataa ggtgatcgtc tgaatcacca gaataaataa
      361 agtcggtgat agcaatccgt aacgataaag taacctgaca gcagaaagtc tccgagcctg
      421 tgcagggtcc caatccggga ttacacatgc tggttaatac cagtaattat aatgagggag
      481 tccaaaaaac aatgaccaac atcaccaaga gaagtttagt agcagctggc gttctggctg
      541 cgctaatggc agggaatgtc gcgctggcag ctgatgtacc cgcaggcgtc acactggcgg
      601 aaaaacaaac actggtacgt aacaatggtt cagaagttca gtcattagat ccgcacaaaa
      661 ttgaaggtgt tccggagtct aatatcagcc gagacctgtt tgaaggctta ctggtcagcg
      721 atcttgacgg tcatccagca cctggcgtcg ctgaatcctg ggataataaa gacgcgaaag
      781 tctggacctt ccatttgcgt aaagatgcga aatggtctga tggcacgcca gtcacagcac
      841 aagactttgt gtatagctgg caacgttctg ttgatccgaa cactgcttct ccgtatgcca
      901 gttatctgca atatgggcat atcgccggta ttgatgaaat tcttgaaggg aaaaaaccga
      961 ttaccgatct cggcgtgaaa gctattgatg atcacacatt agaagtcacc ttaagtgaac
     1021 ccgttccgta cttctataaa ttacttgttc acccatcaac ttcaccggtg ccaaaagccg
     1081 ctatcgagaa attcggcgaa aaatggaccc agcctggtaa tatcgtcacc aacggtgcct
     1141 ataccttaaa agattgggtc gtaaacgaac gaatcgttct tgaacgcagc ccgacctact
     1201 ggaacaacgc gaaaaccgtt attaaccagg taacctattt gcctattgct tctgaagtta
     1261 ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac ttataacaac atgccgatcg
     1321 aattgttcca gaagctgaaa aaagagatcc cggacgaagt tcacgttgat ccatacctgt
     1381 gcacttacta ttacgaaatt aacaaccaga aaccgccatt caacgatgtg cgtgtgcgta
     1441 ccgcactgaa actaggtatg gaccgcgata tcattgttaa taaagtgaaa gcgcagggcg
     1501 acatgcccgc ctatggttac actccaccgt atactgatgg cgcaaaattg actcagccag
     1561 aatggtttgg ctggagccag gaaaaacgta acgaagaagc gaaaaaactg ctggctgaag
     1621 cgggttatac cgcagacaaa ccgttgacca tcaacctgct gtataacacc tccgatctgc
     1681 ataaaaagct ggcgattgct gcctcttcat tgtggaagaa aaacattggt gtaaacgtca
     1741 aactggttaa ccaggagtgg aaaacgttcc tcgacacccg tcaccagggc acttttgatg
     1801 tggcccgtgc aggctggtgt gcagactaca acgaaccaac ttccttcctg aacaccatgc
     1861 tgtcgaacag ctcgatgaat accgcgcatt ataagagccc ggcctttgac agcattatgg
     1921 cggaaacgct gaaagtgact gacgaggcgc agcgcacagc tctgtacact aaagcagaac
     1981 aacagctgga taaggattcg gccattgttc ctgtttatta ctacgtgaat gcgcgtctgg
     2041 tgaaaccgtg ggttggtggc tataccggca aagatccgct ggataatacc tatacccgga
     2101 atatgtacat tgtgaagcac taatggcaat acgtggggca ggagtgtcct gctccacggt
     2161 gtctgatttt tatcgcatta cagaaggcac aggccagaag gtagggcaat gttaaaattt
     2221 attctacgtc gctgtctgga agcgattccg acgctattta ttcttattac tatttcgttc
     2281 tttatgatgc gcctcgcgcc gggaagccct tttaccggcg aacgtacttt accgccagaa
     2341 gtgatggcca atatcgaagc gaaatatcat cttaatgatc cgatcatgac acagtatttc
     2401 agctacctga aacaactggc gcatggcgat ttcggtccat cgtttaaata taaagattat
     2461 tcggtcaatg acctggtggc atccagtttt cccgtttctg ccaaactggg agccgccgca
     2521 tttttccttg cggtaatact gggtgttagt gctggcgtta ttgccgcatt aaaacaaaac
     2581 accaaatggg actataccgt gatggggctg gcaatgaccg gggttgttat ccccagtttt
     2641 gtggttgcgc cattattagt catgatattt gcgatcattt tgcattggct gccgggcggt
     2701 ggctggaatg gtggggcgct taaattcatg atattgccga tggtggcgtt gtcactcgcc
     2761 tatatcgcca gtattgcgcg tattacccgt ggctctatga ttgaagtatt acactccaac
     2821 tttattcgta ctgcccgggc gaaagggtta cctatgcggc gtattatttt acgccacgca
     2881 ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc ccgcatttgt cggcattatt
     2941 accggttcta tggtcatcga aaccatttat ggtttgccgg ggattgggca attgttcgtt
     3001 aatggtgcat tgaaccgtga ctattcctta gtgttaagtc tgaccattct ggttggtgct
     3061 ttaaccattt tgtttaatgc cattgtcgat gtgctatatg cggttatcga cccgaaaatc
     3121 cgttactgat actggagctc gcgatgatgt taagtaagaa aaacagcgag acgctggaaa
     3181 atttcagtga aaagctggag gtcgaagggc gcagcttgtg gcaggacgca cgtcgacgtt
     3241 ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct ggtgctgatc gcgttatttg
     3301 taatcctggc accgatgctt tcgcagtttg cctatgacga tactgactgg gcgatgatgt
     3361 ccagcgcccc ggatatggag tccggtcact actttggtac tgactcatcc ggtcgcgacc
     3421 tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat ggtcggtgtt gctgcggcac
     3481 tggtggcagt ggtcgtgggg acactttacg gttcgctttc cggttatctg ggcggtaaag
     3541 tggattcggt aatgatgcgt ttgctggaaa tcctcaactc cttcccattc atgttcttcg
     3601 tcattttgct ggtgaccttt ttcggtcaaa acatcctgct gattttcgtg gcgattggca
     3661 tggtttcctg gctggatatg gctcgtattg tgcgtgggca aaccctgagt ctgaagcgca
     3721 aagagtttat tgaggcggca caagttggtg gtgtatcgac gccgggcatt gttattcgcc
     3781 acattgtgcc gaacgtactt ggtgtggtgg tggtctacgc atcgcttctg gtgcccagca
     3841 tgatcctctt tgaatctttc cttagcttcc tgggattggg tacgcaagag ccgttaagca
     3901 gctggggtgc attgctgagt gatggcgcga actcgatgga agtctctcca tggttactgt
     3961 tgttcccagc gggattcctc gtggtgacgc tgttttgttt caactttatc ggcgatggct
     4021 tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca gccatgagcg taattgaaac
     4081 tgcaactgtg ccgctcgcac aacaacaggc tgacgcactt ctgaacgtga aagatttgcg
     4141 tgtcaccttt agtaccccgg acggcgacgt cacggcggtc aatgatttga atttttccct
     4201 acgtgccgga gaaacgctgg gcattgtagg tgagtctggt tcgggtaaat cgcaaactgc
     4261 ttttgcgttg atgggcctgt tggctgccaa cggacgtatt ggcggatcgg caaccttcaa
     4321 tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat aaactgcgcg ctgaacaaat
     4381 ctcaatgatt tttcaggacc caatgacttc gttgaatccc tatatgcgcg tcggtgagca
     4441 gttgatggaa gtgctgatgc tgcataagaa catgagcaaa gctgaggcgt ttgaagagtc
     4501 ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcgcgt aaacgcatga aaatgtaccc
     4561 gcacgagttt tctggcggca tgcgtcagcg agtcatgatt gcgatggcat tgctatgtcg
     4621 acctaagctg ctgattgcgg atgaacccac tactgcgctg gacgtcaccg tacaggcgca
     4681 gatcatgacg ctattgaatg aactgaagcg ggaatttaat accgccatca ttatgatcac
     4741 ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg ctggtaatgt acgccgggcg
     4801 cacgatggaa tatggcaacg cgcgcgatgt cttttatcaa cccgttcatc cttattctat
     4861 aggtttgctc aacgcggtgc cgcgtctcga tgcggaaggt gaaacaatgt tgaccatccc
     4921 gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc ccgttccagc cacgttgtcc
     4981 acatgcgatg gaaatttgta gtagcgcacc gccgctggaa gagtttacgc ctggccgtct
     5041 gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg ctgtaaccga aggaagaaaa
     5101 gtcctccttg aaatcgccga tctgaaagtg cactttgaaa tcaaagatgg caaacagtgg
     5161 ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg tcactcttcg cctgtatgaa
     5221 ggggaaacat taggtgtggt aggggaatcg ggatgcggta agtccacctt tgctcgcgcc
     5281 atcattggcc tggttaaagc gaccgacggt catgttgcct ggttaggtaa agagttgctg
     5341 ggcatgaaac ccgatgaatg gcgtgccgtt cgcagtgata ttcagatgat tttccaggat
     5401 ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga tcatcgctga accactgcgt
     5461 acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc gcgtgaaggc gatgatgctg
     5521 aaagtcgggt tattgcctaa cctgattaac cgctatccgc atgagttctc tggtgggcag
     5581 tgccagcgta tcgggattgc gcgtgctctt attcttgaac cgaagctgat tatctgtgat
     5641 gagccggtgt cggcgctgga cgtgtcaatt caggcacagg tggtcaacct tctccagcag
     5701 ctgcaacgtg agatgggatt gtcattaatt tttattgctc atgacctggc cgtggtaaaa
     5761 cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg cggtagaact ggggacctat
     5821 gatgaggtct accacaatcc actacatcct tacaccaggg cattgatgtc ggcagtcccc
     5881 atacctgatc cggatctgga gaagaacaaa accatccagt tactggaagg ggaattgccg
     5941 tcgccgatca acccgccttc cggttgtgtt ttccgtaccc gttgcccgat tgccggtccg
     6001 gagtgcgcca aaacacgtcc tgttctggag ggaagtttca gacacgccgt ttcttgcctg
     6061 aaagtcgatc cgctttaaac acaataaggg ctgacagttg tcagcccttt ttcacgccga
     6121 aagcgattat ttattcccgc cagatgatat ggcaaagttt gtgatctttt tcgcggcata
     6181 acagaatgcg ggcgaaaaca tcgttgattt caccatcttc actgtccgcc agaccaatca
     6241 ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac atgttcctgc cagtcttccg
     6301 ccggatcgaa taactccgcg ccgccgcgct cttcaaactg aagattgaac agcagaacat
     6361 cagctggatc gagattgtcg gcagccagtt cgagaaaaat atcgtaggcc tgctcaagcg
     6421 tttcatcttc agtcaggcga ttgtttagat ccatatccat agtcactacc tgtttaacct
     6481 ctgttggcga cgttttacag caacgggctg aagaagtaaa acagtcgctc ggcgacacgt
     6541 tgccataatg gacgttttag ccataaacgg gcatcgagca gacgtgaacg cgaaatataa
     6601 tcgtcctgaa cggcggcaag gtctgcacca aaacctttat cgtcgattgc cagagtaatc
     6661 tcgaaattta gccacagact ccgcatatca aggttaacgg tgccaaccag acttagttca
     6721 ccatcgacca gcacgctctt ggtatgcagt aacccgcctt caaactgata aattttaacc
     6781 ccagcagcca gcagttccgt aaagaatgcg cgactggccc agccgaccag catcgagtca
     6841 ttttttcgcg gaaggataat actgacatcc accccgcgct gtgccgccgt gcaaatcgca
     6901 tgaagtaaat catcgcttgg cacaaagtag ggcgttgtca tgatcaaata ttcacgcgcc
     6961 gaataagccg cagtcaataa tgcctggtga atgagatctt ccggaaagcc tgggccagaa
     7021 gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg gcataatatt gacatctggt
     7081 ggtggcggca gaatacgttt tccggtttca atctcccagt cgcaggaata aataatcccc
     7141 atcgcggtgg cgatggggcc ttccatacgc gccatcagat caatccattg ccctacgccc
     7201 gcatcttgtt tgaagtagcg agggtcgacc atattcatgc tgccggtgta cgcgatgtaa
     7261 ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca tacggcgtaa aaacacacgc
     7321 atcagattga cctttaaggc ttcgaccact tcaataccgg cattacgcat tagctcgggc
     7381 cacgggctgc ggaaaaaagc cacactcccg gcggagtcga gcatcaatcg gcaatgaatg
     7441 ccgcgtcgcg cagcggccat taatgattca gccacctggt ccgccatgcc gccgggctgc
     7501 cagatataaa acaccatctc aatattatgg cgcgcgagct ggatgtcgcg gattaacgcc
     7561 tgcatcacat catctgactc ggtcatcagt tgtagctgat tccctttgac cccagcgatc
     7621 ccctgacgac gctcgcaaag cttgaataat ggcgcagcga cactgctatt ttcttcggcg
     7681 aagatatgct tacaggcttt aaggtcgtta agccattttg cggtggaagg ccacatcgct
     7741 ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc caacggcaag ataggcaata
     7801 attccgacta acggcagaat gtaaataatc aacagccagg ccatcgcgga gggaactgcg
     7861 cgtcgtttca ttagaatgcg taaagttacg cctgcaatga gcaaccagta tcccagaatg
     7921 gccaaccaac tcaccaacgt ataaacggtt gtcatagatt agaaatcctt ttgaaagcgc
     7981 attgttatga gtttacgcat atggattcat ctggcaaata aaaacgcggg aaaagcactg
     8041 gtcagcgtcg ggggcggggg tataatgacc attctgttat tgcatagagt agttaacatg
     8101 aagcggagta gaacggaagt ggggcgctgg cgcatgcagc gtcaggctag ccgacgtaaa
     8161 tcgcgttggc ttgaggggca atcgcgccga aatatgcgta tccacagcat caggaagtgc
     8221 attctaaaca aacagcgtaa ctcgttattg tttgcgatct acaatatcta aatgtactgg
     8281 gcaccattgc ggtgcccaat aagtttactc ctccgtcttt aaactctatt tttgcgccga
     8341 ttctttactg tcagctttcg tttcttgttc atcgttatcg ctgaaaatac catgacctga
     8401 gttcaacaac attgaaacga gcatatcatt attaatctct tcaccattta aaactcgcgc
     8461 cagaatttcg ctgccaaaca gttgcggtga gagaatgata tccggatgta ccatcttaat
     8521 cttattcagg tttttgctat cactgacggc gagaactgtt ttgacatcac tgctcatatc
     8581 ttttgccgag agtacaacaa acgcgttatc tgcatcgtta tcactcagcg ccagaatggc
     8641 ccggcaacga tcgattcccg ctttctttaa tacagaactg tcattactgt caccggggat
     8701 aacatcagca ttatcgccta agcgttgctc aagttgcttg atatcatctt caggcaagtt
     8761 gctgataacc gttacgtttt gtccgcgttg attcagttgc aggatcgtat tgatggcgag
     8821 aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc attgtatgat tgtttccttt
     8881 tacaagtttg ttgaatcccc cgcggataag cggaccaaaa attgaggtca tagatgtggc
     8941 aaaaacggta atgccggaaa taatgaccga aatagtgaac aatcgtgctg attcagaaac
     9001 agggacaata tcgccatagc cgacggttga catggtttct atcgaaaaat agaacgcggt
     9061 catcaaactt tctattcgcg gattaaaacc ttcgcttaaa taaagcgcac cgtaggttga
     9121 gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt gtcccggctg cggcgctact
     9181 gtgggagaag tctttgcgca gtatcagcaa aaacaccagc gtaaaaatgc aaaatccaat
     9241 actaaatttc agccagggat aaaaatgcag ggtgtaaatt agcgctatca acaacaaaat
     9301 gatactgatt gcccatgcga gctttgcccg gaacagtaag ccaatggagt tcaggacaag
     9361 aaagacgccg agcataaaga gtggggcgtt tgccagcaag gagagatcca actctgaaaa
     9421 tgctttgata tggaaaatat caagcaaatt gaccgacata ccatagattg ttttaaaaat
     9481 aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg tgccgaaggg tcacccataa
     9541 atttgttgct gtttgtttga atgtagccca gtgactcact gatacctctg taaccacaat
     9601 tttgtttaat tatcttagcg tgcacatttt ctgaacatac atgcagcgcg tgaatgtgtt
     9661 aaacaatagt agatgacttt atccgcagta atcaaaggat ttgagcgagc aactgtatct
     9721 caggaaaggt catttttcct gaatatgctc acatcatata aagaaataca gataaagtta
     9781 ttatctgctt gtggtggtga atgcactgac cggctataag gaaaggccaa acaagaacac
     9841 ggttgcaaaa accgtgccct taaatattga atctctattc agaacacttt cttaaatggt
     9901 ttcactgaaa cgtgttcata gactcctgcc gctacgtacg ggtcagcatc ggcccaggcc
     9961 tgagctgctt ccagcgattc aaattcagca ataacggttg agccagtaaa tcccgcagcc
    10021 cctggatcgt tactgtctac cgctggcatt ggaccagctg tcaacaaacg accttcatca
    10081 tgcagtaact gtaaacgtgc taaatgtgcc ggacgaacgg aaaggcgttt ttcgaggcta
    10141 tcagctttgt cttgagcgta aataacatac aacacgggca caactccttg ttcgggaaag
    10201 ttgtaaagta cgttatttga aagggcgaag atctgcaacg gaaagatgat gtctttgtta
    10261 aggccatgca taaattaagg gtaattacgc caaaaatgac attttcactg atcctgatcg
    10321 tcttgcctta ttgaatatga ttgctatttg catttaaaat cgagacctgg tttttctact
    10381 gaaatgatta tgacttcaat gacccttgat ttacctcgcc gcttcccctg gccgacgtta
    10441 ctttcggtct gcattcatgg tgctgttgtg gcgggtctgc tctatacctc ggtacatcag
    10501 gttattgaac tacctgcgcc tgcgcagccg atttctgtca cgatggttac gcctgctgat
    10561 ctcgaaccgc cacaagccgt tcagccgcca ccggagccgg tggtagagcc agaaccggaa
    10621 cctgagccga tccccgaacc gccaaaagaa gcaccggtgg tcattgaaaa gccgaagccg
    10681 aaacctaagc caaaaccgaa gccggtgaaa aaggtacagg agcagccaaa acgcgatgtc
    10741 aaacccgtag agtcgcgtcc ggcatcaccg tttgaaaata cggcaccggc acgcccgaca
    10801 tcaagtacag caacggctgc aaccagcaag ccggttacca gtgtggcttc aggaccacgc
    10861 gcattaagcc gtaatcagcc gcagtatccg gcacgagcac aggcattgcg cattgaaggg
    10921 caggttaaag ttaaatttga cgtcacgccg gatggtcgcg tggataacgt acaaatcctc
    10981 tcagccaagc ctgcgaacat gtttgagcgt gaggtgaaaa atgcgatgcg cagatggcgt
    11041 tatgagccgg gtaagccagg cagtgggatt gtggtgaata tcctgtttaa aattaacggc
    11101 accaccgaaa ttcagtaagc ataaagtcaa aagcctccga ccggaggctt ttgactatta
    11161 ctcaacaggt aaggcgcgag gttttccttc aggatcaacc gcgacatact taaataatgc
    11221 ttctgtcgct ttataccgtt gcccgattgg ttcagacgct acttttttca cccacacttc
    11281 aatattaatg ctgaccgatg tcgtcccttt ctggacacag cgtgcatagc agcacaccac
    11341 atcgccgacc gcaaccggcc gtaagaaagt cattccttca acccgcacag tcactacgcg
    11401 accgtgggca atttcttttg ccagaatagc gccgccaata tccatttgtg acattaacca
    11461 accaccaaag atgtcaccat tggcattggt atcggcgggc atggctaaag tacgtaaaac
    11521 aagatcgccc tgagggacgt tatgtgttgt agacatggta aaaccgactt aaaaggaaaa
    11581 tcacaggcgc gatgctacta tgattttcct gaggagaaca gaggagatac agcgccagcc
    11641 ccgaaggact ggcagtctgg ttaggattta tcttcctgcg gcatgtggcg gtagatatag
    11701 ataccgctta acaatgtgaa gattaaggta agggcggtca ggccaaagac tttaaagttg
    11761 acccaaatat tttgcggcag ccagaatgcg atgtagatgt ttgccagacc gcaaaggata
    11821 aagaaaacag cccaggccag attcagcttc gaccataccg gttgcggcag cgtgagttct
    11881 ttacccagca tccgctgaat tagcggcttt ttcatcaccc attggctgac taacagggct
    11941 cccgcaaata gagcataaat gactgtaacc ttccatttaa taaactcatc attgtggaag
    12001 aacagcgtca agccaccgaa gacgaccacc agaacaaaag tgatcagggc catcttctca
    12061 accttacgaa agcgaaccca gctatagata agcacaatcg ccgtggcgac gatcagcgcc
    12121 gcagtagctg catagatgtc ataaatcttg taaaacgcga aaaagacaac cagcggtaaa
    12181 aaatcaagaa actgcttcat tttacgattc cgtaatcaaa tgcttaaggt tattggcgaa
    12241 tcaacatata caggcggaac agatagatga gcaatatggc tgaaatcagg ttgctcaagg
    12301 tgttcgccag tacggcacca atttccgggg ttaatgcggc aaaagaagag gcaaaaagca
    12361 gcaacagtgt ttttgccagc aaccagctca gtactgcggg tgccaccaga cgcatattcg
    12421 cccaagtcag ccgcatactg ctacgcatcg aggcaaaaat gcccattttg tcctgtacca
    12481 gcatcaccgg tgccagggcc agcaaaatgg ccatgataat tcccggaacg accaccagca
    12541 tgatgccaat ctgtactaaa agggtagtca gaaaaatcag aataaataac ttgggcaata
    12601 tcggcgcact ggcaccaata gctcgcagcg cactgactct ctgacccgca gacaccagct
    12661 ggataattaa tattacgcct ccggcgagaa tggcgttacc gatcaatcct gaaaaagtgg
    12721 aagccgctga agcttgcagc aaaatttgtt gctgttccgg tgacatattc tgaaccaggt
    12781 cgaacaaccc actactgccg ctaacgggta cgccgtcatt gagctgcgca agctgtgcat
    12841 cactgggtga gaaaacatgc cctaacacca ctgtgataaa cgcacatagc aacgatacca
    12901 acagaatggt cataaattga ttacggaaga aatttccggt gtcacggtat acggactgcg
    12961 ccgtgataga catgcactct ccttgagttt tatgcaggtg ttaattagcg ggcaattgta
    13021 ccctggttaa gcctcgggtg gcagcatcag gtcttgtatg gaaaagcata tctttgtaaa
    13081 gcggaggtaa ttgctggcct tcaggcatgg gcaaatcgcg gcaagtggcg accgagttaa
    13141 tatttgcgta gcgaaaatat ttaaaaattg atttaaatca cattaaccag gattctcaat
    13201 gcaacttcta aattaatcca gatcaataaa gggtgaatta tcatatgtaa tgtgatctat
    13261 gtaggatcat ttgttactcc aatgtaggta tattcgtcac gtttttataa ccataacgac
    13321 ggagcggata tgaaaaagtt aacagtggcg gctttggcag taacaactct tctctctggc
    13381 agtgcctttg cgcatgaagc aggcgaattt tttatgcgtg caggttctgc aaccgtacgt
    13441 ccaacagaag gtgctggtgg tacgttagga agtctgggtg gattcagcgt gaccaataac
    13501 acgcaactgg gccttacgtt tacttatatg gcgaccgaca acattggtgt ggaattactg
    13561 gcagcgacgc cgttccgcca taaaatcggc acccgggcga ccggcgatat tgcaaccgtt
    13621 catcatctgc caccaacact gatggcgcag tggtattttg gtgatgccag cagcaaattc
    13681 cgtccttacg ttggggcagg tattaactac accaccttct ttgataatgg atttaacgat
    13741 catggcaaag aggcagggct ttccgatctc agtctgaaag attcctgggg agctgccggg
    13801 caggtggggg ttgattatct gattaaccgt gactggttgg ttaacatgtc agtgtggtac
    13861 atggatatcg ataccaccgc caattataag ctgggcggtg cacagcaaca cgatagcgta
    13921 cgcctcgatc cgtgggtgtt tatgttctca gcaggatatc gtttttaatt ccgcacaaaa
    13981 acgaccccgt aatatacggg gtcaataagg acatggtata aagggtatta tttcttcgct
    14041 tctacgccat cagtttcaga gcgaattaaa aatttctcag ttgtttgcgg aatattctga
    14101 atcagccagt cggccatttg cttttcctca tttaaaatcg cttcgatggt tggaattgaa
    14161 gctgtatcac cggcattttt tgctgctgct aatagtgagg tgtaacaggc gatttcaaat
    14221 tgctcgaaga catatccgct aatagagcct ttgactattt catcagaagg gaatatacca
    14281 ccgattgact gcccaagcgc agccatttta ctcatggaat ctttaatgac tgaacgtgaa
    14341 atgtcattac gatcaagaat agtttccagt tgaacaatct ggtttttggt ttcactaaga
    14401 tgttgttcaa tacgagcgcg tagttcagga taattatcta tacggctggc catggattca
    14461 agcatagatt cggcttgctt ttccattgcg tgggcgtcac gtaaccagtc atgataatgt
    14521 tcaatacgat tcatgaaaat gtcctcttta taaaatgact aaaagttaaa attcatattt
    14581 caggctttat tttcggcttt cttatttacg ttattaatgg ccagatcagt cagtttgatg
    14641 tcggtggcct tttcttcttc cagggtttct ttcagaagct tcgctgcttt acggtaacct
    14701 aattgttcag ccagcgtcgc taatgtcccg taactggcaa tctcataatg ctcgactttc
    14761 tgtgctgcgg caatcagtgc ggcatcacgc acttcgtttt tctcggtact ttcgatgacc
    14821 tcattagctt cttcaataag accttccatt gccacacatt tcatgcgctt aattttcagg
    14881 ttcgattcgg actccacaac ttgatcaata cgttcaatct gtccatgagt ttcctcgagg
    14941 tgcgcatgaa aagcctgact taatttttca tttgatgttg ctcttgcgag ttttgccagt
    15001 gcccgggtta attgtttttc tgcgctgtag gtatctgaaa gcaggtgaat aaatacatct
    15061 tcaatggtct tcatattcat atttttctcc agtgaaatca ctgcgagcca tacggcccgc
    15121 agatgcaatt atctttatca gctgatatta ataattaacg gtgaatcagg atttgcctga
    15181 tttattacca ccgctttgtt gaccgccttt tttacccgct tcagacgcgc gttgcggatc
    15241 atttttaaaa ttaccgccgc tatgctgacc gcctttacgg cctgcgtcgg atgccttctc
    15301 acggtcttcg gcgaaatttc ctgaaccacc acgatgttcg gccatgttat ttctcccgtt
    15361 gcgttgcatt gtttcattaa tatgagtgtt gtgtgtcgac actcattaaa attagtcgct
    15421 aatgagaatt agtcaaatta agcgcaacga gaagatagag ggaaaatata ttttgaggaa
    15481 cattctggat atattaacaa ttacctgagg aata
//
LOCUS       DAJOSY010000097        15271 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJOSY010000097 DAJOSY010000000
VERSION     DAJOSY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.097"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(260..685)
                     /gene="arsC"
                     /locus_tag="OHN27_003363"
     CDS             complement(260..685)
                     /gene="arsC"
                     /locus_tag="OHN27_003363"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCQ2287743.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(698..1987)
                     /gene="arsB"
                     /locus_tag="OHN27_003364"
     CDS             complement(698..1987)
                     /gene="arsB"
                     /locus_tag="OHN27_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCQ2287744.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2041..2394)
                     /gene="arsR"
                     /locus_tag="OHN27_003365"
     CDS             complement(2041..2394)
                     /gene="arsR"
                     /locus_tag="OHN27_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCQ2287745.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            3134..3217
                     /locus_tag="OHN27_003366"
     CDS             3134..3217
                     /locus_tag="OHN27_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCQ2287746.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3271..4623)
                     /gene="gorA"
                     /locus_tag="OHN27_003367"
     CDS             complement(3271..4623)
                     /gene="gorA"
                     /locus_tag="OHN27_003367"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCQ2287747.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4695..5537)
                     /gene="rlmJ"
                     /locus_tag="OHN27_003368"
     CDS             complement(4695..5537)
                     /gene="rlmJ"
                     /locus_tag="OHN27_003368"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCQ2287748.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5740..7782
                     /gene="prlC"
                     /locus_tag="OHN27_003369"
     CDS             5740..7782
                     /gene="prlC"
                     /locus_tag="OHN27_003369"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCQ2287749.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7790..8542
                     /gene="rsmJ"
                     /locus_tag="OHN27_003370"
     CDS             7790..8542
                     /gene="rsmJ"
                     /locus_tag="OHN27_003370"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCQ2287750.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8591..10060)
                     /gene="dtpB"
                     /locus_tag="OHN27_003371"
     CDS             complement(8591..10060)
                     /gene="dtpB"
                     /locus_tag="OHN27_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCQ2287751.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10378..10812)
                     /gene="uspA"
                     /locus_tag="OHN27_003372"
     CDS             complement(10378..10812)
                     /gene="uspA"
                     /locus_tag="OHN27_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCQ2287752.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11203..11538
                     /gene="uspB"
                     /locus_tag="OHN27_003373"
     CDS             11203..11538
                     /gene="uspB"
                     /locus_tag="OHN27_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCQ2287753.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11782..13281)
                     /gene="pitA"
                     /locus_tag="OHN27_003374"
     CDS             complement(11782..13281)
                     /gene="pitA"
                     /locus_tag="OHN27_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCQ2287754.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13513..14715
                     /locus_tag="OHN27_003375"
     CDS             13513..14715
                     /locus_tag="OHN27_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCQ2287755.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAVRGQ"
     gene            14858..>15271
                     /locus_tag="OHN27_003376"
     CDS             14858..>15271
                     /locus_tag="OHN27_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HCQ2287756.1"
                     /translation="MARGRRLKSYLDYENALGDGIGVGYGQSYQPWLRAQDVKSRGNR
                     SIVFGLKTFRNHHLLSSVESNFFYLAEFNDSVIDIREQFPLFPLRLTQLIANHLHFQH
                     PMVRGVRGVPVEVLNVMTTDFLLTLRTPEGGLRYKA"
BASE COUNT         3839 a         3915 c         3998 g         3519 t
ORIGIN      
        1 tctttaaact actgcgctgt atcgctgata actaagcagc tcactgatac accatgctgg
       61 acgcgataat tggaatctca cgcagggagt actgtgtaaa tagcaaaatc gcctgatgcg
      121 ctacgcctgt caggcctaca aataacatac tattcatcga attttcataa ctttgtaggt
      181 cggataagac gcgccagcgt cgcatccgac ataaataaag cgcacttttc taacaacctg
      241 tgggggggat atcgccgctt tatttcaggc gcttacccgc ttcatcaacc actttctcgc
      301 catcttcctt ggagaatgcg cctttttgcg catctggcag aatttccagc accacttctg
      361 aagggcggca caggcgagtt cccagcggcg tcaccacaat cgggcgatta atcagaatcg
      421 ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa tttatcttcc gcaaggccca
      481 gctcctcata cggttcgacg tttttacgca gcagcgcgcg tacggaaatc cccatatcgg
      541 caatgagttt gaccagttca tcgcgcgttg gcggagtttc cagataatgg ataatggtcg
      601 gttctgtgcc gctgttgcgg atcatctcca gcgtattacg cgacgtgccg caggccgggt
      661 tgtgataaat ggtaatgttg ctcatatcag tatctcatta caaagtgaaa gagagacgta
      721 gcgccagcgc agccagcgtc acaaacagca caggcagggt catgataatc cctgtacgga
      781 agtaatatcc ccagctgata gtcatattct tctgcgaaag tacgtgcagc cagagtagcg
      841 tagccaggct accaattggg gtaattttcg gtcccaaatc gcagccaatc acattggcat
      901 aaaccatcgc ttctttgata acgccagatg ccgtgctgcc atcaatggac aacgcgccaa
      961 ccagtaccgt cggcatattg ttcataatag aagagaggaa ggcggtgagg aatccggtgc
     1021 cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt gagtacgcca gaaagatatt
     1081 ccgttaatcc ggcattgcgc aggccataaa ccaccagata catgccgagc gagaagatga
     1141 caatctgcca gggggcaccg cgcaggactt tacccgtatt aatcgcatga ccgcgtttag
     1201 cgacgacaaa taatatcagc gcgcccacag ctgcaatggc gctcaccgga atgccgagcg
     1261 gttccaggac gaaaaatccc accagcagaa gcagtaaaac aacccagcca gttttgaacg
     1321 tagcaggatc tttgatcgct tctgcgggag atttcagcag cgccatatcg tagttctgcg
     1381 gaatatcttt gcgaaaatag agatgtaaca tcaccagcgt ggcaacaatc gcggcgatat
     1441 ccaccggcac catcaccgag gcgtattcgc gaaagccgag gccaaagaaa tcagcggaaa
     1501 cgatattcac caggttggag acaataagcg gcaggctggc ggtatcggca atgaatccgg
     1561 ccgccatcac gaacgccagc gtagtgcctt tactgaaccc taaagccagc agcatggcga
     1621 tgacaatcgg tgtcaaaata agcgccgcgc catcattggc aaacagggcg gcaacggcag
     1681 caccgagcag gacaatccag gtaaacagca agcgaccacg accattaccc cagcgtgaga
     1741 cgtgcagcgc cgcccattca aaaaagccgg actcatccag cagcaggctg atgataatga
     1801 cggcgataaa cgcagccgtc gcgttccaga cgatattcca caccaccgga atatcacccg
     1861 gatggaccac gcccgtaact aacgccagta ctgcgccgag cgttgcactc cagccgatgc
     1921 ctaaaccttt cggctgccag ataaccaata cgatggtcag gacaaagata gcgcctgcca
     1981 gtaacataat gcctcccgga taaaacacat ctgaaaattc atatgtgttt agctaaattt
     2041 ttaactgcaa atgttcttac tgtccccgga acagttttgt cgagccaggt tgcggacaat
     2101 cgcctgaacc ttttcctgtt cacatcgcca ggcctcatca ataattttcg ccgcccatgc
     2161 tggaatatgc ggtgataagc ggtaatgaac ccacttacct tgcttgcggt ccagcaatag
     2221 cccgctttca cgcagcaatg ccaggtggcg ggagatcttg ggctgcgact ggtcgagagc
     2281 agtgcagaga tcgcagacgc ataactctcc cagttcgctg agcagtaaaa cgatgcccag
     2341 acgggtttca tcagcaagaa ttttgaacaa ttggatgggt aacagaaatg acatattgcg
     2401 ctcctgattg ttgcaggtag tgtctctctt cgaagcggat aagtcaaaaa catatatgac
     2461 ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga attttgagtt ggctattcat
     2521 ttgaaaggag gtctgaatca ggacgtaaaa aaaccgactt tgcgtcggtt ttttactttc
     2581 cagccctgag ttggtggctc tggctggagt gagagagttc ttatctaaca gctcaatact
     2641 attagccagt ggctaacatt gcaagaatta gcgatttctt cagctggcgg agggagcact
     2701 tcggtatata tcgcagtgac gatttatata ctttcactgg gtcatcgtca tattaagcct
     2761 tgtccgaatc cggatgtaaa aaaaccgact ttgcgtcggt tttttacttt ccagccctga
     2821 gttggtggct ctgactggag tgagatagcc atcatctaac gcatcaatgc taataccata
     2881 ctgaaactat tgcaaggacg tgctggtttt ataacctgca tgtactgtat gattatccag
     2941 ttagctctga ggcattttca ctctggcaat gcgcataaac gctttcaaag tcctggtcag
     3001 aagtacgggt ggtgccgtta actgatgctc tggccggagt gagagagttc ttatctaaca
     3061 atgagacatg cgccgtgaca ggcagtggat gagtaagcgg atgcattctc actccatcgc
     3121 atggagaaaa cgggtgattg ataaagcaat catcgttcta ggggcgttaa ttgcgctgct
     3181 ggaactgatc cgctttctgc ttcagcttct gaactgatag cggaaacgta attaagggct
     3241 aagagcacac tactcttagc cctttaacat ttaacgcatt gtcacgaact cttctgccgc
     3301 cgttgggtga atggcgacgg tattgtcgaa gtcttttttg gttgccccca tcttcagcgc
     3361 caccgcgaag ccctgcaaca tttcgtccat accaaagcca atgccgtgaa taccgacaat
     3421 cttctcttcc gatccaacgc acaccagctt catgcggcac ggctggcggt gagtggtgac
     3481 ggcggtatac atcgcggtga aagaggattt atacactttc acctgatcgt cgccatactg
     3541 ctcgcgcgcc tgcggttccg ttaaaccaac agtaccaatc ggcggatggc tgaagaccac
     3601 ggtcggaatg ttgctgtaat ccagatgctc atccggcttg ttattaaaca ggcgttcaga
     3661 gagacggcga cccgctgcaa ctgccaccgg tgtcagctcc actgcacccg tgttatcgcc
     3721 caccgcgtaa ataccttcaa tattggtgtt ttgatattta tcgacgacga tatagccttt
     3781 ttcgttagtt ttaacgccag cggcttccag gttgatgttg tcattggcag gctcgcgacc
     3841 aatcgcccaa atcaggcaat ccaccgtttc actgcgacca tcttccagct ccagcgtcag
     3901 gctaccatcg gcatttttca ctaccgcttt cgggatggcg ttggtgtgca gctgcgggcc
     3961 ttcggcgttc atcacttcga ccagcgtttc ggaaatcatc gggtcgaagc tgcgcagcgg
     4021 cgcatgttta cgcacaaaca gatgcgtttt cgcgccgagg ccgttaatca cgcccgccag
     4081 ctcaacggcg atgtaacccg cgccaacaac cgccacgcgc tctggcaaag caggaagggc
     4141 gaagaagcca tcagaatcaa taccgtattc cacgcccgga atatccgggt ggctcggacg
     4201 accgcctgtg gcgatcagaa tatgatcggc cgtgatggtt tcgccgttta cctccagcgt
     4261 tttggcatca acgaagcggg caaagccttt gattacatca acgttatttt taccgagcac
     4321 gttttcatag gaagtatgaa tacggtcgat ataggcggta cggctggcga tcaacgtttc
     4381 ccagttgaat ttattgatag tggtatcaaa accataatcc gggccgtaca tatggatcgc
     4441 ttcacggatt tgcgccgcgt gccacatcac ttttttcggc acacagccaa catttacgca
     4501 ggtgccgccc agctctttgg cttcaatcag cgcacatttc tggccgtaca tagccgcgcg
     4561 gttgatggag gcgataccgc cgctgccgcc gccgatggcg atgtaatcat agtgtttagt
     4621 catgacaaag tgtccttatc gttgattacc gcgattgtag cgcgagacgt aataggtgcc
     4681 agcaatggct gcaattactc cggcacgatc cagcttacgg tggcgtgccc ggtgcctgcc
     4741 ggaaccagtt tgctgtgcag ccacggcagc acgttattca tctgttgttc cagtttccac
     4801 ggcgggttaa tcacaatcat gccggaagcg gtcatgccac ggcgatcgct gtctggcagt
     4861 accgccagtt caatttgcag aattttgcga ataccggtcg cttccagatc gtggatcatg
     4921 cgcttaattt gctgacgcag caccaccgga taccacagtg cgtaagtacc agtggcgaaa
     4981 cgtttgtaac cttctgctat cccgctgacc accgcttgat agtcagtttt catttcatac
     5041 ggcgggtcga taaggattaa accacggcgg gaaaccggcg gcagtttggc cttaagctgc
     5101 tggaaaccgt cggctttttc gacacgcgca cggctatctt tctgaaattc agaacgcaac
     5161 aacgggtaat cgctcgggtg cagttcggtc agttgcaggc tgtcctgttc acgcagtagc
     5221 tggcgagcaa tcaacggcga accggggtag taacgcaact gaccgctacg gttgaagtgt
     5281 tttaccacat tgatgtacgc ctccagttct gcgggcaaat cgtcctgctg ccagatacgg
     5341 gcgatgcctt cgagatattc gccggtacgc tcggcatgtt cgctgcctaa ctgataacgc
     5401 ccggcccctg cgtgggtgtc gagatagaga aacggtttat ctttctcttt cagcgactcg
     5461 atgatcaggc tctgaacggt atgtttaagg acgtcggcgt ggttgccagc gtgaaagctg
     5521 tggcgataac tgagcatggg taaagatgtt ccggtaagta aaaatcggcc cgtattcggg
     5581 gcgcacaaaa gcgtatcagg acagtatacc gaaaagaggc cgccgccgcg aaagcgtaac
     5641 gtttctcatt gaaattcact acacttaacc ccatgctaca cacattatgt aaagcgcctg
     5701 ttgagcgctt ccttaacctc tttaaccagg actgcgcgaa tgacgaatcc gttactgact
     5761 ccctttgaat tgcctccgtt ttctaaaatt ctcccggaac atgtcgttcc agccgtgact
     5821 aaggcattga acgactgccg cgaaaacgtg gagcgcgtag tagcgcaagg ggcaccgtac
     5881 acctgggaaa atctctgcca gccgttggcg gaagtggacg atgtgttggg gcgtatcttc
     5941 tccccggtca gccacctgaa ctcggtgaaa aatagcccgg aactgcgtga agcgtacgaa
     6001 caaaccctgc cgctgctgtc ggaatacagc acctgggtag ggcaacatga agggctgtat
     6061 aaagcgtacc gcgacctgcg cgatggcgat cattacgcca cgctgaacac ggcgcagaag
     6121 aaagcggttg ataacgccct gcgtgatttt gaactctctg gcataggtct gccgaaagag
     6181 aaacagcagc gttacggcga aattgctacc cgtctttctg aactgggcaa ccagtacagc
     6241 aacaacgtcc tcgatgcgac aatgggctgg accaaactcg ttaccgacga agcggaactg
     6301 gcggggatgc cagaaagcgc gctggctgcg gcaaaagccc aggctgaagc gaaagaactt
     6361 gaaggctacc tgctgacgct ggatatccca agctacctgc cggtaatgac ctactgcgac
     6421 aaccaggctc tgcgtgaaga gatgtatcgc gcttacagca cccgcgcctc cgatcaaggc
     6481 ccgaacgccg gtaagtggga caacagcaag gtgatggaag agatcctcgc gctgcgtcac
     6541 gaactggcgc aactgctggg ctttgaaaac tacgccttca aatcacttgc cactaaaatg
     6601 gcagaaaacc cgcagcaggt gctggatttc ttaaccgatc tggcaaaacg cgcgcgtcca
     6661 caaggcgaaa aagagctggc gcaattgcgt gcctttgcca aagccgaatt tggcgtcgat
     6721 gagttgcagc cgtgggatat cgcttactac agcgaaaaac aaaaacagca cctctacagc
     6781 atcagtgacg aacagctgcg tccgtacttc ccggaaaaca aagcggttaa cggcctgttt
     6841 gaagtggtta agcgtattta cggcatcacc gctaaagagc gtaaagatgt tgatgtctgg
     6901 catccggatg tacgtttctt cgaactgtat gacgaaaata acgaactgcg cggtagcttc
     6961 tacctcgatc tgtatgcccg tgaaaacaag cgcggcgggg cgtggatgga tgactgcgta
     7021 ggccagatgc gtaaagctga tggttctttg caaaaaccgg tcgcgtattt gacttgtaac
     7081 ttcaaccgcc cggtaaatgg taaaccggcg ctgttcactc acgacgaagt gatcaccctc
     7141 ttccacgagt tcggtcacgg cctgcaccat atgctgaccc gcatcgaaac cgctggtgtt
     7201 tccggtatca gcggtgtgcc gtgggatgcg gtcgaactgc cgagtcagtt tatggaaaac
     7261 tggtgctggg agccggaggc gctggcgttt atctctggtc actatgaaac cggcgaaccg
     7321 ctgccgaaag agttgctgga taaaatgctg gcggcgaaga actaccaggc ggcgctgttt
     7381 attctgcgtc agctggagtt cggcctgttt gatttccgcc ttcatgccga gttccgcccg
     7441 gatcaggggg caaaaatcct cgaaactctg gcagaaatca agaaactggt tgccgtggtg
     7501 ccatctccgt cctggggccg tttcccgcac gctttcagcc atattttcgc cggtggttat
     7561 gccgcaggtt actacagcta cctgtgggct gacgtactgg cggcagatgc tttctcgcgc
     7621 tttgaggaag agggcatttt caaccgtgaa accgggcagt cgttcctcga caacattctg
     7681 agccgtggcg gttcagaaga gccgatggat ctgttcaaac gcttccgtgg tcgtgaaccg
     7741 cagctggatg cgatgctgga gcattacggc attaagggct gatcattcag tgaaaatctg
     7801 cttaattgat gaaacaggca ccggagacgg tgccttatct gttctggcgg cccgctgggg
     7861 gctggagcac gatgaagaca acctgatggc gctggtgtta acgccggaac atctggaatt
     7921 gcgcaagcgt gatgagccaa aacttggcgg catctttgtt gattttgttg gcggagcgat
     7981 ggcgcaccga cgcaaattcg gcggtggtcg cggtgaggcg gtggcgaaag cggtgggcat
     8041 taaaggcgat tatttgccgg atgtggtgga tgccactgca ggacttgggc gcgatgcctt
     8101 tgtactggct tcagtcggct gccgcgtgcg gatgctggag cgtaatccag tggttgccgc
     8161 gctactcgac gacggcctgg cgcgtggtta tgcggatgcg gaaatcggcg gctggttgca
     8221 ggagcggttg cagttaattc acgcctccag cctgacggcg ctgactgata ttaccccgcg
     8281 cccgcaggtg gtttatctcg acccgatgtt cccgcataag cagaaaagcg cgctagtgaa
     8341 aaaagagatg cgtgtctttc agtcgctggt gggaccggat cttgatgccg atggattact
     8401 ggagcctgca cgcttgctgg cgaccaaacg cgtggtggta aagcgcccgg actacgcgcc
     8461 accgctggcg aatgtcgcca cgccaaacgc ggtagtcact aaagggcatc gctttgatat
     8521 ctatgcaggt acgccagtgt aaaactaaaa cagggtagcg ataacgctac cctgctttct
     8581 gcaagaataa ttaatggctt tccggcgtcg caatcatgcg tttcagccac ggcaccatca
     8641 acagcatcac tactgcaacg cccagcgtga ccagaccaat cttaccaaac acgttggtat
     8701 agacgggcaa cgtctcaagc ggatcggtaa tgttgtccgg caccgcggta aatgttgcca
     8761 catagccgcc cagcaagaac gcggcagcct gcgtcaggaa ccacatcccg agaataaagc
     8821 ccatcaaatg ctgcggcacc agggcagcaa tcatcgccag gccaagggcg ctaataaaca
     8881 gttcacctaa gctctggaat aagtacacca gcacgataaa ccatggcgat gtcagccctt
     8941 gtgcatccgc aaaccacatt cccgcagctg ccgccgtcaa aaagcccagt gagcacataa
     9001 acatgccgag agtaaatttc atcggcatcg agaggtcttt gcctttgtta cccagatgcg
     9061 tgtaaatgcc tgccagtatt gggctggcga gtaccaccca gaacgggtta agcgcctgga
     9121 agctgaccgg gttgatggaa aaaccgagaa tttcatgatg cacgttgttg atggcaaaga
     9181 agttcagcga tgttggcatc tgggcgtaga gaatgtaaaa caccaccgct tcgagcatca
     9241 ggacaaaggc gacaaacatt ttattgcgcc cggttttatc cagcttgaat gcctgacgaa
     9301 agaagatgat ggtgacgacg atggagagaa caatcagcac cagattggcg acttctacgt
     9361 tgtgcatcag ccatgcgcat acgaagatca tcaccacgct gccaagtaac acgtacaaca
     9421 gtttgctgaa gctcattggc cggaagtcgg gttcagaacc aatgtctttc accattccac
     9481 gacaggcgat gtaaaccagt aatgcgataa ttaaccccgc cccgcacagg ttgtaggtga
     9541 ctgaataacc gaatctatca gcgatcacag gggccagcga taacgctatc aacgagccga
     9601 tgttgatcga catatagaac agggtgaatg cgccatcaag ccgcggatct ttcggcggat
     9661 agcacttcga aagcaagctg gctgggttag ctttaaacag gccgttacct acagcgatag
     9721 tccccagggc gatgaaaatc aggtcaggct taagtagcga catgccggtc atgaagtagc
     9781 caatcgccag cacaagtgca ccaagaacaa tggtgcgttt ggtccccagc aggtggtcgc
     9841 cgacatagcc gccaatggaa atgaggccat agaccagcgc agcaaaagca ccaaaagtga
     9901 caaaagcctg ctcttgcgag aatccaagct gtttaacgaa gaaaaccgcc agtacgccct
     9961 gcacgccgta gtagccgaat cgctcccata actcgacaaa aaagatcatg aaaaatgggc
    10021 gaggttgctg cagcatcccc atgggtgttg ttgtattcat atttattaac cttccaatac
    10081 catcctgaaa agtatgaacg cgtaagggat aacgcgcgaa aaagtttaca tctgtcagag
    10141 attcttaata ttgatgcggt gaatagatta atacactatt acaacagaaa ataaccagct
    10201 gatcacacta atgatggaaa atatgatggt gttaaatatt gtattggctg gggattttga
    10261 ttgcgaacgc agaccgtagg ccagataagg tgtttacgct ggatcaggta acaaggcgcg
    10321 ccaaaaagca aaacgcccgc atatgcgggc gttcaggaac acgtcgtaga gggaagatta
    10381 ttcttcttcg tcgcgcagcg gaacaatcag catatcaacg tgaacggtgt tgatcagctg
    10441 acgtgcggaa gacatcagtt tgctccagaa gtcctggtgg tgaccacaaa ccaccagatc
    10501 catatcgtat ttcttgattg catcgaccag aacttggccc aggtcgccgc tgccgctcag
    10561 ggtttcagtg attgggtagc ctgcattagt ggaaagctcg gtcagcgcat gatgtgtctc
    10621 ttctgagatg cgtttctgca tatcacccag attcacatca ataagcccgg tgtataggtc
    10681 agagtagttt acatctacgt ggatcagaga aactttcgca ttgtaggggc gagccataga
    10741 gactgctttc tctaccagaa ctttgctttc cggggagaga tcgaccgcga tgagaatgtg
    10801 tttataagcc atagtgttac tccttccata aagttgtcga tgactggcca gctagcgttt
    10861 cttgtgccgt tacgtcaccc gcgtcctgcg aactacgtgc gcggggctaa cccagccgta
    10921 aagattcagt gttaagacca tccttacctt atagcgaccc ggatgatccg tcaatccgcc
    10981 ttgcttaccc gttagataaa caaattttcg acatgaatat attgatagtg gttaaccttc
    11041 tggaaaaaaa acaacctgat ctcctacact atctatagag ccgctcggat gttggtcacc
    11101 acaaaagcgg tttctccagg acctggtgtt aactgattgg cagcgtatcg ggagcggtag
    11161 ccccggggtg gaactccgcg ggcaggtcgc cggggaggag gtatgataag caccgtcgca
    11221 ttattttggg ctttatgtgt cgtttgcatt gttaacatgg cgcgctattt ctcatcacta
    11281 cgcgcgttgt tagtggtact gcgtaactgc gatccattgc tctatcaata tgttgatgga
    11341 gggggctttt ttacctcaca tggccaaccc aacaaacagg tgcgtctcgt ttggtatatc
    11401 tatgcccaac gttatcgcga tcatcacgat gatgaattta ttcgccgctg tgagcgggtg
    11461 cgtcggcagt ttattctgac cagcgcattg tgtggtctgg tggtggtcag cctgattgca
    11521 ttgatgattt ggcattaagc acttcagcaa taataacgca taaaaaaagc gggccagtaa
    11581 actgacccgc tcgagactga ttacaaacgc aaaattgcct gatgcgctac gcttatcagg
    11641 cctacataat ctctgcaatc tattgaattt gcatgttttt gtaggccgga taaggcgttc
    11701 acgccgcatc cggcatgaac aaagcgcact atgtcacaat ctgaagcggg ccagctgacc
    11761 cgctcgtttt ggtgcgtacg attacaggaa ctgcaaggag agccagtaca gcccgccgga
    11821 aagcagtacc gcagccggaa gggtaaacac ccaggccatc agaatgctgg tcacggtttt
    11881 acgctgtaag ccgccaccat ctaccaccat cgtccccgcg acagaagagg agagtacgtg
    11941 agtggtggaa accggcatcc cggtataact cgccaggccg atagacactg ccgccgtcat
    12001 ctgggcagac atcccctgag cgtaggtcat gcctttctta ccgattttct caccgatagt
    12061 cgttgccact cgacgccagc caatcatcgt accgatacct aacgccagcg cgaccgccat
    12121 gatgatccac accggtgcat actcgatggt gctaagcatg tcggacttca gtttcttcaa
    12181 caggcgctga tcgtcagcac tcacgccagg catcttcacc actttgtcga tagtgtcaga
    12241 aacgcacagc ataatgcggc gcatctggct acgttgatca agcgacagct tgtcgtagct
    12301 ttccacatcg gtggtcaaca tacctttcag gcggttgagc gcgttaatgg tattcgacgg
    12361 atggcagtgg aactccgcag gttgcgttgc gccagcttcc ggagccggta ctaactgatc
    12421 agcaccggta gcctgtttga gcagcgcagg atgctgctca aagtaagctt cgacgttgtt
    12481 gatggcatca cgggtacggg tgatttcgta gccagtggca ttcatgttca ccacgaagcc
    12541 tgctggcgcg acgccaatca ataccaacat aaccagacca atgcctttct gaccatcgtt
    12601 cgcgccgtgc gaaaacgcca cgccgatagc ggaaaggatc agcgcgatac gcgtccagaa
    12661 tggcggcttt ttcttgccgt ctttcttttc acgctccgct ggggtcaggt ggatacgggc
    12721 gcgtttcttg gtgccgctcc agtagcgacg cagcaagaaa atcagaccgc cagcaaacac
    12781 caggccgaca ataggggaaa cgatcagaga accgaaaata cttaatactt tcgggatatt
    12841 gagtgcatcc accactgacg tcccggtcat caacgcattg gttaaaccaa tcccgatgat
    12901 cgcgccaatc agcgtatgag agctggatgc aggtaaacca aagtaccagg tacccaggtt
    12961 ccagataatc gccgccagca acatagagaa caccatggca aggccatgag acgatcccat
    13021 attaagcagc agatccgtcg gcagcatatg cacaatggca taggcaacac tcagaccacc
    13081 cagcaaaaca cccaaaaagt tgaacaccgc cgccataacc acggcgagct gagaacgcat
    13141 cgcgcgggta tagataacgg ttgccacggc gttggctgtg tcatggaaac cattgatggc
    13201 ttcgtagaac agcacaaaag ccagtgcaag caataataac agcccggtat gcaaatccag
    13261 gccagcaaac aaatgtagca taggacgtta cgccattttg aggacatgaa cgcggcgcat
    13321 tatcagtgac tttcgcggcg cgggcaaagt gaaatataga ctttttttga tttgcctcct
    13381 gtatggattt cactcaaaaa ataattatct tatataattc aggtaaatac ttccttttag
    13441 taatattgat gctggtgcga ccactgagga atctttacaa ttcacgcccg ttttttctaa
    13501 gaggagcgca acgtggaaag gtttgatgcc attattatag gcgctggtgc ggcgggtatg
    13561 ttctgttctg cgctggcagg tcaggcagga cgccgggttc tgctgatcga taatggtaaa
    13621 aaaccagggc gcaaaatcct tatgtctggc ggtgggcgct gcaactttac caacctttat
    13681 gtcgaaccag gcgcttatct gagccagaat ccgcattttt gtaagtctgc actcgcgcgt
    13741 tttacccagt gggatttcat tgatctggtc aataaacacg gcatcgcctg gcacgagaaa
    13801 acgttaggac aactcttctg cgatgactcc gcgcagcaga ttgtcgacat gctggtggat
    13861 gagtgcgaga agggcaatgt aaccttcaga ttgcgtagcg aagtgctgag tgtggcgaag
    13921 gatgaaacag gcttcacgct tgaactgaac ggcatgactg tcggttgcga aaagctggtc
    13981 atcgcgaccg gtgggctgtc aatgccgggg ctgggcgcgt cgccgtttgg ttataagatt
    14041 gccgaacaat ttggcctcaa cgtgctgccg acccgtgcgg gtctggtgcc attcactctg
    14101 cataaaccgt tgctcgaaga gttacaggtg ctggcgggcg tggcggtgcc ttccgtgatt
    14161 accgctgaaa acggcatcgt tttccgtgag aacttactct tcacccatcg cggcttgtct
    14221 ggaccggcgg tgttgcagat ttcaagctac tggcaaccgg gtgaatttgt cagtatcaat
    14281 ctgctacctg atgtggacct cgaaaccttc ctgaatgagc agcgtaacgc acatccgaat
    14341 cagagcctga aaaacacact ggcggttcat ctaccgaagc ggttggttga acgcttacag
    14401 caactcgggc aaatcccgga tgtttcgcta aaacagctca acgtgcgtga ccaacaggca
    14461 ctgattagca cattgaccga ctggcgcgta caacccaacg gcactgaagg ctatcgcact
    14521 gccgaagtga cgctcggcgg cgtggacacc aacgaactct cttcacggac gatggaagcg
    14581 cgcaaagtgc ctgggctgta cttcatcggc gaagtgatgg acgtcaccgg ctggctgggg
    14641 ggctataact tccagtgggc gtggtcgagt gcatgggctt gtgcgcagga tttgattgct
    14701 gtccgaggac aataaagttg tacacaaagc actaaagttg taaccattct caaaaaacga
    14761 cgctaatgtt gtatacttac tgaatgtgtg gttatagggt tgaagactat aaaatgcaca
    14821 tataggtacc tatcaatgtc gtttttaggg tacgatcatg gctaggggta ggcgtctcaa
    14881 atcctatttg gattatgaaa atgcgctagg tgacggcata ggagtgggct atggccaaag
    14941 ttatcagccc tggcttagag ctcaggacgt taaatcccgt ggaaaccgtt cgatagtctt
    15001 tggccttaag acgtttcgaa accatcatct cctttcttct gtcgaaagta actttttcta
    15061 tctggctgag tttaatgact cggtgattga tatccgggaa caattcccac tctttcctct
    15121 ccggcttacc caactaatag caaatcatct acattttcaa catcctatgg tgaggggagt
    15181 aagaggagta cctgtcgaag ttctgaatgt tatgacaacc gattttttac tgaccttgag
    15241 aactcctgaa ggcggacttc gatacaaagc t
//
LOCUS       DAJOSY010000098        15097 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJOSY010000098 DAJOSY010000000
VERSION     DAJOSY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.098"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            12..989
                     /locus_tag="OHN27_003377"
     CDS             12..989
                     /locus_tag="OHN27_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCQ2287757.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVDQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQTAFKWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1059..1541)
                     /locus_tag="OHN27_003378"
     CDS             complement(1059..1541)
                     /locus_tag="OHN27_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCQ2287758.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1777..4359
                     /gene="leuS"
                     /locus_tag="OHN27_003379"
     CDS             1777..4359
                     /gene="leuS"
                     /locus_tag="OHN27_003379"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCQ2287759.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRIWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4374..4955
                     /gene="lptE"
                     /locus_tag="OHN27_003380"
     CDS             4374..4955
                     /gene="lptE"
                     /locus_tag="OHN27_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCQ2287760.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4955..5986
                     /gene="holA"
                     /locus_tag="OHN27_003381"
     CDS             4955..5986
                     /gene="holA"
                     /locus_tag="OHN27_003381"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCQ2287761.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEHLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5988..6629
                     /gene="nadD"
                     /locus_tag="OHN27_003382"
     CDS             5988..6629
                     /gene="nadD"
                     /locus_tag="OHN27_003382"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCQ2287762.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6653..7264
                     /locus_tag="OHN27_003383"
     CDS             6653..7264
                     /locus_tag="OHN27_003383"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCQ2287763.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAATPLTARGIEQAQNLHTLLHD
                     VFFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7524..7841
                     /gene="rsfS"
                     /locus_tag="OHN27_003384"
                     /gene_synonym="rsfA"
     CDS             7524..7841
                     /gene="rsfS"
                     /locus_tag="OHN27_003384"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCQ2287764.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7845..8312
                     /gene="rlmH"
                     /locus_tag="OHN27_003385"
     CDS             7845..8312
                     /gene="rlmH"
                     /locus_tag="OHN27_003385"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCQ2287765.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8343..10244
                     /gene="mrdA"
                     /locus_tag="OHN27_003386"
     CDS             8343..10244
                     /gene="mrdA"
                     /locus_tag="OHN27_003386"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCQ2287766.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10247..11359
                     /gene="mrdB"
                     /locus_tag="OHN27_003387"
                     /gene_synonym="rodA"
     CDS             10247..11359
                     /gene="mrdB"
                     /locus_tag="OHN27_003387"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCQ2287767.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11370..12458
                     /gene="rlpA"
                     /locus_tag="OHN27_003388"
     CDS             11370..12458
                     /gene="rlpA"
                     /locus_tag="OHN27_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCQ2287768.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12597..13808
                     /gene="dacA"
                     /locus_tag="OHN27_003389"
     CDS             12597..13808
                     /gene="dacA"
                     /locus_tag="OHN27_003389"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCQ2287769.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13919..14182
                     /locus_tag="OHN27_003390"
     CDS             13919..14182
                     /locus_tag="OHN27_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCQ2287770.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14283..14924
                     /gene="lipB"
                     /locus_tag="OHN27_003391"
     CDS             14283..14924
                     /gene="lipB"
                     /locus_tag="OHN27_003391"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCQ2287771.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3723 a         3817 c         3952 g         3605 t
ORIGIN      
        1 aggaaatgat aatgattttc acgtcaagtt gctgcgataa tttatcaata gatgagatta
       61 tcgaacgtgc tgaaaaaggc gattgcgagg ctcaatatat tgttgggttt tattataatc
      121 gcgatagcgc aattgattct ccggacgacg aaaaagcctt ttactggctg aagctggccg
      181 ctgagcaagg tcattgtgaa gcacagtatt ccttagggca aaagtatacc gaggataaaa
      241 gccgtcataa agataatgag caagccatct tctggctgaa aaaagctgcc ctacaaggcc
      301 atactttcgc ttccaacgcc cttggctgga cactggatcg tggagaagcc cccaattata
      361 aagaagcggt tgtctggtat cagatagccg cggagagcgg aatgtcttat gcgcaaaata
      421 atcttgggtg gatgtacaga aatggcaacg gagtcgcaaa agactatgcg ctggcatttt
      481 tctggtacaa acaagctgca ttacaaggcc atagtgacgc gcaaaacaat ctggccgatc
      541 tttatgaaga cggaaaaggc gttgatcaaa acaagacact cgccgcattc tggtatttga
      601 aaagcgcaca gcagggtaat cggcacgccc agtttcaaat tgcatgggat tataacgctg
      661 gcgaaggggt ggaccaggac tataagcaag cgatgtactg gtatctgaag gctgccgctc
      721 aggggagcgt cggcgcttac gtcaacatcg gttatatgta taaacacgga caaggcgttg
      781 agaaagatta tcagactgcc tttaaatggt ttacgaaagc cgctgaatgc aatgacgcca
      841 ctgcctggta taacctggcc attatgtatc actacggaga aggaagacct gtcgatctcc
      901 gacaggctct cgacctgtat cgtaaagttc agtcatccgg aaccagagat gtcagtcagg
      961 aaattcgtga gactgaagat ttactgtaga gataaaaaca agggccgggg aatttcattc
     1021 ggcccatatt ctctttattg ctgtgcgaaa tcagactatt acggctcaaa cgggcgtctc
     1081 tggaactggt catgaccaca tttcgggcat agcgtcagca cttccggtgt gtagatcggg
     1141 agatggaagt gacatttctc gcagaccaga tttcccagcc cgaccacttc tccgctgtga
     1201 taaaccccat gatgattgag gtcctggaaa acttcgcgcc attcaagctg cgttttatcg
     1261 gtgatgtctg ccagctcctg ccacaagctt tctttaatca cccgcataaa gacgctgtca
     1321 gattcttctt tcaggctctc ttcatagctc atggcgaact cttccaggtc acgtctgaca
     1381 gctcgcgtca gctcatcgac ctcggttcgc gttaactccc ctgtttttat tacgcgctcg
     1441 cgcgcctgtt ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg ttcgctcagt
     1501 gacgcaacca gttcacggta atattgagca accttgttca tcgtttcatc tcccgggtcg
     1561 ttaactgact ataataatag acgttctttg ttccttgctt tgctaatacg cccacaaact
     1621 ctgtaaatta atgtatgaat aatccgggca tcatttcaac gtcgagtgcg cgaaaggctg
     1681 ttttgacgcg gacgtttggg ctatgctatg cggatctgaa aaaccacctc taacgctact
     1741 tttgtagccg taatgaaaac aggaccactg gctgccatgc aagagcaata ccgcccggaa
     1801 gagatagaat ccaaagtaca gcttcattgg gatgagaagc gcacatttga agtaaccgaa
     1861 gacgagagca aagagaagta ttactgcctg tctatgcttc cctatccttc tggtcgacta
     1921 cacatgggcc acgtacgtaa ctacaccatc ggtgacgtga tcgcccgtta ccagcgcatg
     1981 ctgggcaaaa acgttctgca gccgatcggc tgggacgcgt ttggtctgcc tgcggaaggc
     2041 gcggcggtga aaaacaacac tgcaccggca ccgtggacgt acgacaacat cgcgtatatg
     2101 aaaaaccagc tcaaaatgct gggctttggt tatgactgga gccgcgagct ggcaacctgt
     2161 acgccggaat actaccgttg ggaacaaaaa ttcttcaccg agctgtataa aaaaggcctg
     2221 gtatataaaa agacttctgc ggtcaactgg tgcccgaatg accagaccgt actggcgaac
     2281 gaacaagtta tcgacggctg ctgctggcgc tgcgatacca aagttgaacg taaagagatc
     2341 ccgcagtggt ttatcaaaat caccgcttac gctgacgaac tactcaacga tctggataaa
     2401 ctggatcact ggccagacac cgttaaaacc atgcagcgta actggatcgg tcgttccgaa
     2461 ggcgtggaaa tcaccttcaa cgttaacgac tatgacaaca cgctgaccgt ttacactacc
     2521 cgcccggaca cctttatggg ttgtacctac ctggcggtag cagcgggtca tccgctggcg
     2581 cagaaagcgg cggaaaataa tcctgaactg gcggccttta ttgacgaatg ccgtaacacc
     2641 aaagttgccg aagctgaaat ggcaacgatg gagaaaaaag gcgtcgatac tggctttaaa
     2701 gctgttcacc cattaacggg cgaagaaatt cccgtttggg cagcaaactt cgtattgatg
     2761 gagtacggca cgggcgcagt aatggcggtt ccggggcacg accaacgcga ctacgagttt
     2821 gcctctaaat acggcctgaa catcaaaccg gttatcctgg cggctgacgg ctctgagcca
     2881 gatctttctc agcaagccct gactgaaaaa ggcgtgctgt tcaactctgg cgagttcaac
     2941 ggtcttgacc atgaagcggc cttcaacgcc atcgccgata aactgactgc gatgggcgtt
     3001 ggcgagcgta aagtgaacta ccgcctgcgc gactggggtg tttctcgtca gcgttactgg
     3061 ggcgcgccga ttccgatggt gacgctggaa gacggtaccg taatgccgac cccggacgac
     3121 cagctgccgg tgatcctgcc ggaagatgtg gtgatggacg gcattaccag cccgataaaa
     3181 gcagatccgg agtgggcaaa aactaccgtt aacggtatgc cagcgctgcg tgaaaccgac
     3241 actttcgaca cctttatgga gtcctcctgg tactatgcgc gctacacttg cccagagtac
     3301 aaagaaggta tgctggattc cgaagcggct aactactggc tgccggtgga tatctacatt
     3361 ggtggtatcg aacacgccat tatgcacctg ctctacttcc gcttcttcca caaactgatg
     3421 cgtgatgcag gcatggtgaa ctctgacgaa ccagcgaaac agttgctgtg tcagggtatg
     3481 gtgctggcag atgccttcta ctatgttggc gaaaacggcg aacgtaactg ggtttccccg
     3541 gttgatgcta tcgttgaacg tgacgagaaa ggccgtatcg tgaaagcgaa agatgcggca
     3601 ggccatgaac tggtttatac cggcatgagc aaaatgtcca agtcgaagaa caacggtatc
     3661 gacccgcagg tgatggttga acgttacggc gcggacaccg ttcgtctgtt tatgatgttt
     3721 gcttctccgg ctgatatgac tctcgaatgg caggaatccg gtgtggaagg ggctaaccgc
     3781 ttcctgaaac gtatctggaa actggtgtac gagcacacag caaaaggtga tgttgcggca
     3841 ctgaacgttg atgcgctgac tgaagatcag aaagcgctgc gtcgcgatgt gcataaaact
     3901 atcgctaaag tgaccgatga tatcggccgt cgtcagacct tcaacaccgc aattgcggcg
     3961 attatggagc tgatgaacaa actggcgaaa gcaccaaccg atggcgagca ggatcgcgct
     4021 ctgatgcagg aagcgctgct ggccgttgtc cgtatgctta acccgttcac cccgcacatc
     4081 tgcttcacgc tgtggcagga actgaaaggc gaaggcgata tcgacaacgc gccgtggccg
     4141 gttgctgacg aaaaagcgat ggtggaagac tccacgctgg tcgtggtgca ggttaacggt
     4201 aaagtccgtg ccaaaatcac cgttccggtg gacgcaacgg aagaacaggt tcgcgaacgt
     4261 gctggccagg aacatctggt agcaaaatat cttgatggcg ttactgtacg taaagtgatt
     4321 tacgtaccag gtaaactcct caatctggtc gttggctaag cgcgggagga agcgtgcgat
     4381 atctggcaac attgttgtta tctctggcgg tgttaatcac cgccgggtgt ggctggcatc
     4441 tgcgtgatac cacgcaggtt ccttccacta tgaaggtcat gattctggac tcaggcgatc
     4501 cgaacgggcc attaagccgt gcggtgcgta accagttacg tctgaatggt gtcgagttgc
     4561 ttgataaaga aaccacgcgt aaggacgttc catccctgcg tttgggtgct gtatcaatct
     4621 cgcaggatac cgcgtcggtt ttccgtaatg gtcaaactgc ggaatatcag atggtgatga
     4681 ctgttagcgc gtcggtattg attcctggac gagatatcta cccgattagc gccaaagtct
     4741 tccgttcgtt cttcgataac ccgcaaatgg cgttagcgaa agataacgag caggaaatga
     4801 tcattaaaga gatgtacgac cgtgctgccg aacagctgat tcgtaagcta ccaagcatcc
     4861 gtgctgcgga tattcgttcc gacgaagaac agacgtcgac cacaacggat actccggcaa
     4921 cgcctgcacg cgtctccacc acgctgggta actgatgatt cggttgtacc cggaacaact
     4981 ccgcgcgcag ctcaatgaag ggctgcgcgc ggcgtatctt ttacttggta acgatcctct
     5041 gttattgcag gaaagccagg acgctgttcg tcaggtagct gcggcacaag gattcgaaga
     5101 acaccacact ttttccattg atcccaacac tgactggaat gcgatctttt cgttatgcca
     5161 ggccatgagt ctgtttgcca gtcgacaaac gctattgctg ttgttaccag aaaacggacc
     5221 aaatgcggcg atcaatgaac acctcctcac gctcaccgga ttgctgcatg acgatctgct
     5281 gttgatcgtc cgcggtaata aattaagcaa agcgcaagaa aatgccgcct ggtttactgc
     5341 tcttgcgaat cgcagcgtgc aggtgacctg tcagacaccg gagcaggctc agcttccccg
     5401 ctgggttgct gcgcgcgcaa aacagctcaa cttagaactg gatgacgcgg caaatcaggt
     5461 gctctgctac tgttatgaag gtaacctgct ggcgctggct caggcactgg agcgtttatc
     5521 gctgctctgg ccagacggca aattgacatt accgcgcgtt gaacaggcgg tgaatgatgc
     5581 cgcgcatttc accccttttc attgggttga tgctttgttg atgggaaaaa gtaagcgcgc
     5641 gttgcatatt cttcagcaac tgcgtctgga aggcagcgag ccggttattt tgttgcgcac
     5701 attacaacgt gaactgttgt tactggttaa cctgaaacgc cagtctgccc atacgccact
     5761 gcgtgcgttg tttgataagc atcgggtatg gcagaaccgc cggggcatga tgggcgaggc
     5821 gttaaatcgc ttaagtcagc cgcagttacg tcaggccgtg caactcctga cacgaacgga
     5881 actcaccctc aaacaagatt acggtcagtc agtgtgggca gagctggaag ggttatctct
     5941 tctgttgtgc cataaacccc tggcggacgt atttatcgac ggttgatatg aaatctttac
     6001 aggctctgtt tggtggcacc tttgatccgg tgcactatgg tcatcttaaa cccgtggaaa
     6061 cgctggcgaa tttgattggt ctgacgcggg tcacaatcat ccctaataat gttcctccgc
     6121 atcgtcccca gccggaagcg aacagcgtgc agcgtaaaca catgcttgaa ctggcgattg
     6181 ccgacaagcc attatttact cttgatgaac gcgagctaaa gcgcaatgcc ccctcttaca
     6241 ctgcgcaaac actgaaagag tggcggcagg aacaaggacc ggacgtgccg ctggcgttta
     6301 ttattggtca ggattcattg ctgacctttc cgacctggta cgaatacgaa acgatactcg
     6361 acaatgcaca tttgatcgtc tgtcggcgtc caggttaccc acttgaaatg gcgcaaccgc
     6421 aataccagca atggctggaa gatcatttga cacataaccc ggaagatctt caccttcagc
     6481 ctgccggtaa aatttatctg gctgaaacgc cgtggtttaa catctcggcg accatcatcc
     6541 gcgaacgttt gcaaaacggt gaatcgtgtg aggatttatt gccggaaccg gtattgacgt
     6601 acattaacca acaaggcttg tatcgctgat acctgtcgtt acggagggca tgatgcgact
     6661 gtggttaatt cgtcatggcg aaacacaagc gaatgtcgat ggtctgtaca gcggtcatgc
     6721 ggccacaccc ctgaccgcac gcggtattga gcaagcgcaa aatctgcata cactgctaca
     6781 tgatgttttc tttgacctgg ttttatgcag tgaactggaa cgggcacagc ataccgcgcg
     6841 actggttctc agtgaccgcc agctccccgt tcatatcatc cctgaactca acgaaatgtt
     6901 ttttggcgac tgggagatgc gacatcatcg cgacctcatg caagaagatg ccgaaaacta
     6961 tagcgcgtgg tgcaatgact ggcagcatgc tatccccacg aacggtgaag gatttcaggc
     7021 attttcgcaa cgtgtggaac gctttatcgc aaggcttagt gaatatcagc actatcagaa
     7081 tattttaatc gtcagccatc agggcgtact gagtctgtta attgctcgtt taattggcat
     7141 gcctgccgag tctatgtggc attttcgcgt tgaccagggg tgttggagcg ccattgatat
     7201 caaccaaaaa ttcgcgacgc tacgcgtcct caatagccgt gccatcgggg tcgagaatgc
     7261 atgacttttc tgttttttta cgggtaagcc gcaacgacca ttgacagccc cgggcaggct
     7321 gatattctcc gcagccagac tttttccgcc agacacgact ttgtagaaat tgttttacaa
     7381 aaatggcgat gcaatctgcg gcgcggggtg ggatgatagc ccactttcga aagccgattc
     7441 ggcgacaatt gtcccgaaat cgcctctggt tcaggtatac tgacagacca tttttatcta
     7501 tttgattcac ccagggggaa aacttgcagg gtaaagcact ccaggatttt gttatcgaca
     7561 aaattgatga cctcaaaggt caggacatca tcgccttaga cgttcagggc aaatccagca
     7621 tcaccgactg catgatcatc tgtacgggta cgtccagccg tcatgttatg tccattgctg
     7681 accacgttgt gcaggagtct cgcgcagcgg gcctgttacc gctcggcgta gaaggtgaaa
     7741 acagcgccga ctggattgtc gtggatttgg gcgatgtgat tgtccatgtc atgcaggaag
     7801 agagccgtcg cctgtatgaa ctggaaaaac tctggagtta atgcgtgaag ctgcaacttg
     7861 tcgccgtggg aacgaaaatg ccggactggg tacaaaccgg ttttaccgag tacctgcgtc
     7921 gttttccgaa agatatgccc ttcgagctga ttgaaattcc ggccggaaaa cgcggcaaga
     7981 atgcggacat caagcgcata ctcgacaaag agggtgagca gatgttggcg gccgcaggca
     8041 aaaaccgcat tgtcaccctc gatattccag gcaagccctg ggatacgccg cagttagccg
     8101 ctgagctgga acgctggaag ctggatggtc gcgacgtcag tctactgatt ggcgggcctg
     8161 aagggttgtc gcctgcctgt aaagcggcgg cggagcagag ctggtcgctg tcggcgctta
     8221 ccctccccca tccgctggtt cgcgtgctgg tcgcagagag tctgtaccgg gcgtggagca
     8281 tcaccaccaa ccatccttat caccgtgagt gataagggag ctttgagtag aaaacgcagc
     8341 ggatgaaact acagaactct tttcgcgact atacggcaga gtccgcgctg tttgtgcgcc
     8401 gggcgctggt cgcctttttg gggattttgc tgctgaccgg cgtgcttatc gccaacctgt
     8461 ataatctgca aattgttcgc tttaccgact accagacccg ctctaatgaa aaccgcatta
     8521 agctggtgcc tatcgcgccc agccgcggca ttatctacga ccgtaacggt atccctctgg
     8581 cactcaaccg cactatctac cagatagaaa tgatgccgga gaaagtcgat aacgtgcagc
     8641 aaacgctgga cgctttgcgc agcgtggttg atctgaccga tgacgatatt gctgcattcc
     8701 gaaaagagcg cgcacgttca caccgtttca cctctattcc ggtgaaaact aacctgaccg
     8761 aagtacaagt agctcgcttt gccgtcaatc agtaccgttt tccgggtgtt gaagttaaag
     8821 gctataaacg tcgttactat ccttacggtt cggcgttgac ccacgtcatc ggctatgtgt
     8881 cgaaaatcaa cgataaagac gtcgaacgcc tgaataatga cggcaaactg gccaactatg
     8941 cggcaacgca tgatatcggt aagctgggca ttgagcgtta ctatgaagat gtgctgcacg
     9001 gtcagaccgg ttatgaagag gttgaagtta acaaccgtgg gcgtgttatt cgccagttaa
     9061 aagaagtacc accgcaagcc ggacacgata tttacctgac gctggatctc aaactccagc
     9121 aatatattga aacgctgctg gcgggtagcc gcgcagctgt ggtagtcacc gatccgcgta
     9181 caggtggggt gctggcgctg gtttccacgc ctagttatga cccaaacttg tttgttgacg
     9241 gtatctccag caaagattat tccgccttgt tgaacgaccc gaatacaccg ctggtgaacc
     9301 gcgccacaca gggggtttat cctcccgcgt ctacagttaa accctatgtg gcggtttcgg
     9361 cattgagcgc cggggtgatc acgcgcaata cgacgctgtt tgacccaggc tggtggcaac
     9421 tgccaggttc ggaaaaacgt tatcgtgact ggaaaaaatg gggccacggg cgtctgaatg
     9481 tcacaagatc gctggaagaa tctgcggata ccttcttcta tcaggtggcc tacgatatgg
     9541 ggatcgatcg cctctccgaa tggatgggta aattcggtta tggtcattac accggtatcg
     9601 acctggcgga agaacgttcc ggcaacatgc ctacccgcga atggaaacag aaacgcttta
     9661 aaaaaccgtg gtatcagggt gacaccattc cggttggtat cggtcagggt tactggacag
     9721 cgaccccaat ccagatgagt aaggcactga tgatcctgat taatgacggt atcgtgaagg
     9781 ttcctcattt gctgatgagc accgccgaag acggcaaaca ggtgccatgg gtacagccgc
     9841 atgaaccgcc cgtcggcgat attcattccg gttactggga gctggcgaaa gacggtatgt
     9901 acggtgttgc taaccgccct aacggtacgg cgcataaata ctttgctagc gcaccgtaca
     9961 aaattgcggc gaaatccggt accgctcagg tcttcggtct gaaagcgaac gaaacctata
    10021 atgcgcacaa aattgccgag cgtttacgtg accacaaact gatgaccgcc tttgcgccat
    10081 acaacaatcc gcaagtggct gtcgccatga ttctggagaa cggtggtgcg ggtccggcgg
    10141 ttggtacact gatgcgccag atcctcgacc acattatgct gggtgataac aacaccgatc
    10201 tgcctgcgga aaatccagcg gttgccgcag cggaggacca ttaatcatga cggataatcc
    10261 gaataaaaaa acattctggg ataaagtcca tctcgatccc acaatgctgc tgatcttact
    10321 ggcattgttg gtttacagcg ccctggttat ctggagcgcc agcggtcagg atattggcat
    10381 gatggagcgt aaaatcggcc aaatcgcgat gggtctggtc atcatggtgg tgatggcgca
    10441 aattcctcca cgcgtttatg aaggctgggc cccctatctc tatatcatct gtattatttt
    10501 gctggtggcg gtagatgctt tcggtgccat ctctaaaggt gctcaacgct ggctggacct
    10561 cggtattgtt cgttttcagc cgtcggaaat tgccaaaata gccgtaccac tgatggttgc
    10621 gcgctttatc aaccgcgacg tttgcccgcc atcgttgaag aacactggca tcgcgctggt
    10681 gctgatattt atgcccacgc tgctggtggc tgcacagcct gacctgggaa catcaatcct
    10741 cgttgcgctt tccggtctgt ttgtactgtt cctctctggc cttagctggc gtctgattgg
    10801 cgtcgcagta gtgctggtgg cggcgttcat tccgattctg tggttcttcc tgatgcatga
    10861 ttaccagcgc cagcgcgtaa tgatgctcct ggacccagaa tcagacccac tcggcgcggg
    10921 ctatcacatt attcagtcta aaattgctat tggctccggc ggattacgcg gcaaaggctg
    10981 gctgcacggc actcagtcac agcttgaatt tctccccgaa cgccataccg actttatctt
    11041 cgcggtactg gcggaagagc tgggattagt gggcattctg attctgctcg ctctctacat
    11101 tctgctgatc atgcgcgggc tgtggatagc cgccagagcg caaaccacct ttggtcgcgt
    11161 catggctggt ggcttaatgc tgatattatt cgtttatgtc ttcgtaaata ttggtatggt
    11221 aagcggtatt ctgccggttg taggggttcc gctcccactg gtcagttatg gaggatcggc
    11281 gctaattgtg ctgatggctg ggttcgggat tgtaatgtca atccacaccc acaggaaaat
    11341 gttgtcgaaa agcgtgtaag aggtgcgcaa tgcgtaagca gtggctcggg atctgcatcg
    11401 cggcaggaat gctcgcggca tgtacaagcg atgatggtca gcaacagacg gtaagtgtac
    11461 cgcagcctgc ggtatgtaac ggccccatag ttgaaattag cggggcggac ccgcgtttcg
    11521 aaccactgaa cgcgacggca aatcaggatt accagcgcga cggtaaaagc tacaaaatcg
    11581 tgcaggatcc gtctcgattt agccaggcgg gactggcggc aatctatgat gccgaaccag
    11641 gcagtaacct gacggcctct ggcgaagctt tcgatccgac acagctgacg gcggcccatc
    11701 caacgcttcc gatccccagc tacgccagaa tcactaacct ggctaacggg cgaatgatcg
    11761 tggtgcgcat taatgatcgc ggcccttacg gcaacgaccg cgttatttcg ctttctcgcg
    11821 cggcagctga ccgtcttaac acgtcaaaca acaccaaagt tcgtatcgat ccgattattg
    11881 tcgcccagga tggttcgctt tctggtcctg gtatggcttg taccacagtc gccaaacaga
    11941 cttacgccct gcctgcacct cccgatttaa gcggtggcgc gggaacaagt tcagtgtctg
    12001 gcccgcaggg tgacattctt ccggtcagta attcgacgct aaaaagcgaa gatccgaccg
    12061 gcgcgccggt aaccagtagc ggtttcctcg gcgctccaac gaccttagcg cctggtgtac
    12121 tggaaggcag cgaaccgacg cctgcgccac agcccgttgt tacagctccg tcgacaacgc
    12181 ctgcaacctc gcctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc agcggcaact
    12241 ttatggtgca agtcggggcc gtaagcgatc aggctcgtgc gcaacagtac caacagcaac
    12301 tgggacagaa gttcggcgtc cccggtcgcg taactcaaaa tggcgcggtc tggcggatcc
    12361 agcttggccc attcgccagc aaagccgaag ccagtacctt gcagcaacgt ttgcaaaccg
    12421 aagcccaatt acagtcattt attactaccg cacagtagcg taaagcaggc atctgatgtg
    12481 tcaatttctg taaatgacgt taacaaagta acgcggaaag tcagatgcct gccggtagtg
    12541 catttgctat agtagggcac ttttttaatt ccatcacgga tgtcgtagtt cagaccatga
    12601 ataccatttt ttccgctcgt atcatgaagc gcctggcgct caccacggct ctttgcacag
    12661 cctttatctc tgctgcacat gccgatgacc tgaatatcaa aactatgatc ccgggtgtac
    12721 cgcagatcga tgcggagtcc tacatcctga ttgactataa ctccggcaaa gtgctcgccg
    12781 aacagaacgc agatgtccgc cgcgatcctg ccagcctgac caaaatgatg accagttacg
    12841 ttatcggcca ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc actatcggca
    12901 acgacgcatg ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg ttcctcaaac
    12961 cgggcatgca ggttccggtt tctcagctga tccgcggtat taacctgcaa tcgggtaacg
    13021 atgcttgtgt cgccatggcc gattttgccg ctggtagcca ggacgctttt gttggcttga
    13081 tgaacagcta cgttaacgca ctgggcctga aaaataccca cttccagacg gtacatggtc
    13141 tggatgctga tggtcagtac agctccgcgc gagatatggc gctgatcggc caggcattga
    13201 tccgtgacgt accgaatgaa tactcgatct ataaagaaaa agaatttacg tttaacggta
    13261 ttcgccagct gaaccgtaac ggcctgttat gggataacag cctgaatgtc gacggcatca
    13321 aaaccggaca cactgacaaa gcaggttaca accttgttgc ttctgcgact gaaggccaga
    13381 tgcgcttgat ttctgcggtg atgggcggac gtacttttaa aggccgtgaa gccgaaagta
    13441 aaaaactgct aacctggggc ttccgtttct tcgaaaccgt taacccactg aaagtaggta
    13501 aagagttcgc ctctgaaccg gtttggtttg gtgattctga tcgcgcttcg ttaggggttg
    13561 ataaagacgt gtacctgacc attccgcgtg gtcgcatgaa agatctgaaa gccagctatg
    13621 tgctgaacag cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc ggaactatca
    13681 acttccagct tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg caagaaatcc
    13741 cggaaggtaa cttcttcggc aaaatcattg attacattaa attaatgttc catcactggt
    13801 ttggttaaaa attgaacact tgaaagtgta atttccgtcc ccatatacta agcatcagta
    13861 aaaaaactcc cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat
    13921 gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg
    13981 gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg
    14041 tgactacacc ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac
    14101 tatcaacgcc actcatatcg agcaggttga aacactgtat gaagaactgg gcaaaatcga
    14161 tattgtccgc atggttctgt aacgcgcttc tccgttaccc ggctcctggt cgggtaactc
    14221 ccctctcctc gctgtgatat acttttcccc cacttttact cattctccac ggagatgccg
    14281 ttttgtatca ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatct
    14341 cccaggctat gcatgaattc accgataccc gcgatgatag tacccttgat gaaatctggc
    14401 tggtcgagca ctatccggta ttcacccaag gtcaggcagg aaaagcggag cacattttaa
    14461 tgccgggtga tattccggtg atccagagcg atcgcggtgg gcaggtgact tatcacgggc
    14521 cggggcaaca ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg
    14581 aactggtgac cttgcttgag caaacagtgg tgaataccct ggctgaactg ggtatagaag
    14641 cgcatcctcg ggctgacgcg ccaggtgtct atgttgggga aaagaaaatt tgctcactgg
    14701 gtttacgtat tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc
    14761 tttcaccatt tttacgtatt aatccttgtg ggtatgccgg aatggaaatg gctaaaatat
    14821 cacaatggaa acccgaagcg acgactaata atattgctcc acgtttactg gaaaatattt
    14881 tagcgctact aaacaatccg gacttcgaat atattaccgc ttaattccat acatcaatgg
    14941 cccaatttac actgggtcat tactcataat atctgctaat gctttacatc tccttcgcgt
    15001 ttgtatattt tctttatcac ttgcagaaat gcggtgccac gccttgctcc gtaactcacc
    15061 ccattgatta acctattgaa atagttgatt atggtga
//
LOCUS       DAJOSY010000099        14964 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJOSY010000099 DAJOSY010000000
VERSION     DAJOSY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.099"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            196..1290
                     /gene="ddlA"
                     /locus_tag="OHN27_003392"
     CDS             196..1290
                     /gene="ddlA"
                     /locus_tag="OHN27_003392"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCQ2287772.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1314..1526)
                     /locus_tag="OHN27_003393"
     CDS             complement(1314..1526)
                     /locus_tag="OHN27_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCQ2287773.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1786..2094
                     /locus_tag="OHN27_003394"
     CDS             1786..2094
                     /locus_tag="OHN27_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCQ2287774.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2153..3247)
                     /gene="yaiW"
                     /locus_tag="OHN27_003395"
     CDS             complement(2153..3247)
                     /gene="yaiW"
                     /locus_tag="OHN27_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCQ2287775.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3260..4480)
                     /gene="sbmA"
                     /locus_tag="OHN27_003396"
     CDS             complement(3260..4480)
                     /gene="sbmA"
                     /locus_tag="OHN27_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCQ2287776.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSTHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4832..5989
                     /gene="ampH"
                     /locus_tag="OHN27_003397"
     CDS             4832..5989
                     /gene="ampH"
                     /locus_tag="OHN27_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCQ2287777.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5990..6613)
                     /gene="iprA"
                     /locus_tag="OHN27_003398"
     CDS             complement(5990..6613)
                     /gene="iprA"
                     /locus_tag="OHN27_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCQ2287778.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6701..9703)
                     /gene="ehaB"
                     /locus_tag="OHN27_003399"
     CDS             complement(6701..9703)
                     /gene="ehaB"
                     /locus_tag="OHN27_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HCQ2287779.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVDVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTI
                     ASWDDVALAVVAHPNADNAMQTTADFSNSTLMGDVIFSSNFDENFFPRGADSYRDADG
                     EVDTNGWDGTDRLDLTLNNGSKWVGAAQSVHQTGSIDVDGDGKGDIATYGVGTEATAT
                     LIDIEDNSLWPLSTVGVENDDTSYSEFDHITGNQVYQSGLFNVTLNTGSQWDTTKTSL
                     IDTLSINSGSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEV
                     SAGWAVGDAALYANNIKVTNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNI
                     ESNRFVLDADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEV
                     DNNGDHVAAATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAY
                     TYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNG
                     GAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDR
                     SGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWG
                     FGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAG
                     YTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNF
                     SAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            10226..11200
                     /gene="hemB"
                     /locus_tag="OHN27_003400"
     CDS             10226..11200
                     /gene="hemB"
                     /locus_tag="OHN27_003400"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCQ2287780.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(11358..12209)
                     /gene="tauD"
                     /locus_tag="OHN27_003401"
     CDS             complement(11358..12209)
                     /gene="tauD"
                     /locus_tag="OHN27_003401"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCQ2287781.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(12206..13033)
                     /gene="tauC"
                     /locus_tag="OHN27_003402"
     CDS             complement(12206..13033)
                     /gene="tauC"
                     /locus_tag="OHN27_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCQ2287782.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(13030..13797)
                     /gene="tauB"
                     /locus_tag="OHN27_003403"
     CDS             complement(13030..13797)
                     /gene="tauB"
                     /locus_tag="OHN27_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCQ2287783.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQNNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(13810..14772)
                     /gene="tauA"
                     /locus_tag="OHN27_003404"
     CDS             complement(13810..14772)
                     /gene="tauA"
                     /locus_tag="OHN27_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2287784.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         3738 a         3895 c         3626 g         3705 t
ORIGIN      
        1 taagccagga ataaattagc ttcccgttca tcatttttgt gatcttgcgc acatctttga
       61 gataacgcac tttcttcacc agtacgttgc acgctttgtc agcagaagat tgcaacaatc
      121 tttcagaagg ccgcgaatcc cccgttttgt cgtggtaatg tatgcacttt gtgttagacg
      181 ggataggttt ttaagatgga aaaactgcgg gtaggaatcg tttttggtgg taaatcagcg
      241 gaacatgaag tgtctctgca atcggcaaaa aacattgtcg atgccattga taaaagtcgc
      301 ttcgacgttg tgctgctggg cattgataaa caagggcaat ggcacgtcag cgatgccagc
      361 aattatctgc taaatgcaga cgatcctgcc catattgcgt tgcgcccttc ggcgaccagc
      421 cttgcgcagg tgccaggtaa acatgagcat cagcttatcg acgcgcaaaa cggtcagccg
      481 ttgccgacgg tggatgtcat tttcccgatt gtccacggta cgctgggcga agatggttcc
      541 ttgcagggaa tgctgcgggt cgccaattta ccgtttgtag gttctgatgt tctggcttca
      601 gcagcctgta tggataaaga tgtcaccaaa cgtctgctac gcgatgccgg gctgaacatt
      661 gcgccattta ttaccctgac gcgcgctaat cgtcacaaca tcagttttgc cgaagtggag
      721 tctaaactgg ggttaccact gtttgtaaaa ccggctaatc agggctcttc tgttggtgtc
      781 agcaaagtaa ccagtgaaga acagtacgca attgccgtcg atctggcgtt cgagttcgat
      841 cataaagtga tcgttgagca agggatcaaa ggtcgtgaga tcgaatgcgc agttctgggc
      901 aacgacaatc cgcaagccag cacctgtggc gagatcgtac tcaccagcga tttctatgcc
      961 tacgacacca agtacattga cgaagatggc gcgaaagtgg tagttccggc agccattgca
     1021 ccagagatca acgataagat ccgggcgatt gccgttcagg cttatcagac gttgggatgc
     1081 gcaggcatgg cgcgtgtaga cgtgttttta accccagaga acgaagtggt gatcaacgag
     1141 atcaacacac tgcctggctt caccaacatc agtatgtatc cgaagctgtg gcaagccagc
     1201 ggtctgggtt acaccgatct gatcacacgt ttgattgagc tggcgctgga gcgtcacgct
     1261 gcggataacg cattgaaaac cacaatgtaa tattttaacg cccgataata ttcttattgg
     1321 gcgttttcag tttcttcatc gcgacgacga ataatcaacc ccgccagcca gaaactaatc
     1381 acccacgtca ccagaccgac ggcataggtc tgccaacctt ttgcttcaaa tcccagtaac
     1441 cccaccacgc cattaagaat gaatataagg ccaatggcga acgcatagta gtgccagtca
     1501 cggcggattt ttacaggcag attcatggca gctccgttaa cgaaaaacaa catccttgca
     1561 tattctgtaa caaccgtctg tggtgtagac aacattttcg taattgttaa taaatttaac
     1621 ttaaggttgc cattttgtga ctcacaacga agattcacgc ttcgggaata ttcccaacat
     1681 caatttacca tgattctgat attgacaatc taaatgacct gtcagacttg ccataagccg
     1741 ctggtatcac accagaagag aagcagatga cttccggagg tttttatggc tgatttcacc
     1801 ctgtcaaaat cgctgtttag cggaaaatat cgcaatgcct cttcaacgcc tggcaacatt
     1861 gcctatgcgt tgtttgtgct gttttgcttt tgggctgggg cgcaattgct gaacctgtta
     1921 gtgcatgcgc ccggcgtcta tgagcgttta atgcaggtcc aggaaacagg tcgcccacgg
     1981 gtggaaattg gtttaggtgt cggcaccatt ttcgggctga tcccgttttt agtaggctgc
     2041 ctcatttttg cagtggtggc gctatggctg cactggcgac atcgccgcca gtaagattta
     2101 cgttgccaga tacggcctaa aacgccttat ctggcatgac attcctgttt gatcactgtt
     2161 tcatacaacg cgcccgccgt tcgtctacgc gcttcgcaaa ccaggccgta gtcaggttgc
     2221 gggtgatttt cgggctttcc agttgaatgc caggcaacat ttcgcggggt agtgatttac
     2281 ccgttttcgc ttcggcaagt tgataaactt tcttgtacag cgccgtctcc tcaaaagaga
     2341 agctgtcacc tttctctaac tgacggcgaa tttcgctgtc gctcatcccc agttttcccg
     2401 ccagtttgcg cgttgccagt tcggttttcc cgggttcttt actgtcgtaa cgaattaaat
     2461 caccgtccag tgccagcttc acgccgctgg ccttactgac tgcgttctga aacgcggcat
     2521 tacggctggc gtaccagcca gcgttaaaat cagcaaagcg gtatatcggt gcgctatagc
     2581 tggcgggata attcagtaaa tggtaagtac caaaccacaa cccgccgcgg cgactgaaga
     2641 cttcctgacg aactgtaccg tccattttcc acggataccc tttggtatgc tgttcggcaa
     2701 aagcaatgct gacctgcatc ggcccaccgg tgcgcaccgg attaagcgaa ccaaacagcg
     2761 tctgccccat tggtaccatg ctgatcagat cgtcaaaaat cgcgctcaat tgcttttctg
     2821 tacgcaccga atctaaccgt tcgctataac ttttgccgtt tggcgatttg atttttagcg
     2881 cggtatgaac cagaaaagca ggaatgtgca tccgttcagc acgacggtca atctcttgcc
     2941 aggcgatctt gcttaagccc ggaacggccg gatctgcctg ataatttgac tcctgctgcg
     3001 ccaccgccag caccgagcag acgttttcca gcgtcggtgc cagcccctga ctttcaaagg
     3061 tggtggcgag atctttcgcc caggcatcgc ggtctttcac gctcgcaggc atcttctgcc
     3121 gcacgacgct cgccacatct atcgcttttt cgcccttttt caacggttgc ggagcctgac
     3181 tactacaccc cgctaaaacc agaaccgcca acaatgacag cgaagataga ggattgacgc
     3241 gcgacattac ttctcctttt tagctcaagg tatgggttac ttcctgaatt ttgtcaccat
     3301 ccagttcatg ttcaaagctg cgcagacgtt tgtagataga catcaactca accagtgtgg
     3361 tccatgagtt aatcaggtac tggaaagcac cgcgaacctg accaaaaacg ttggtaatct
     3421 gcgtcatcag gccgagcgta atcgtaccgg caacaattga cggaaacagc aagaacaaac
     3481 cgaaaacgtt atcgacctgc aaatagagaa tgcgggcgat gttgaaatac atatagtgaa
     3541 aatagaggcg gaaatagttt ttccgcacgg cgctaaacag ctcgcgtacc gtaggcggcg
     3601 tcgcacgcgt ggcatcgtct tcaccataaa ccagctcttt acggtaggcg gcctctactc
     3661 gctggttttt aaactccagc cccggcagtt tgatccctac cactgccagc aatccggtcc
     3721 ccatcagcga ccagacgata gcggcaatca ccagaccata cggaatgtgc ccgataatcg
     3781 gcagctccgg cacatgcgtg gagagcgtta ccagcaccgg caggaaggcg atcaacgtca
     3841 tgatggcgtt gataaaactg acgcccatat tctccagcgt tgaagcaaaa cgcatggtgt
     3901 cttcctgcac acgctgtgcg gccccttcga tatgacgcag ttgttgccag ttcgccatgt
     3961 aatattcgtt catcgccgta cgccagcgga acacgtagtg actgacaaag aagttgttca
     4021 gcacactgat caccacagcg atcagcgcaa tccccagaaa gacgcccact tcgcggtaaa
     4081 attgttcgat agtgacttta tgcggcgaac ttagcgcggt ttgaatcaga tcatagaacg
     4141 gtgcatacca ggcgttgacg gcgaccccga cttccaccaa aaaccaggtg acgaagatga
     4201 tcagtgcagt accgagtatt gaccagtatt gccaacggtg cgggctgtag ataaaccaga
     4261 aaaatgcaaa aagtcctacg caaacaatgt agtaagcgta aaaaatcagg aaatccaacg
     4321 accagaaacg cgcggcgcta atcgggatct gcccggaagc gccagtgata cgcgccaccc
     4381 agtctccccc accggcttgc cagaagataa cggcaatcaa tgcccaaaca aaggccgaga
     4441 gaaaaaacgc tcccggcttt gggaaaaaag acttaaacat atgcactcct gctaacttct
     4501 tatcgtttta acaactgctg tgtattaacc gcatgaccac tacaaagcgt cagccaaatg
     4561 aaaccgccca ttgtgaccca tagcaaggcg cttagttcgc cagcctgacc ataagaattg
     4621 tttcgacgga tttcacaaac caaagattgt ttacataagc ggattaacac gcgcaggatc
     4681 acaaatgcag gcattggtga gcaggaaaaa gatcgtgttt acgcgccgtt acgtttttaa
     4741 cactattatt gcactaattc gccctttgca atccatcaat gagtagtata aatacgctca
     4801 gttaccttca ttcaatttat ggacaccacc gttgaaacgt agtctgcttt tttctgccgt
     4861 gctgtgtgcg gcgtcattga cctccgtcca tgcggcgcag ccgatcactg aaccggagtt
     4921 tgcctctgat attgtcgatc gttatgccga tcatattttt tacggcagcg gtgccacggg
     4981 gatggcgctg gttgttatcg acggtaacca gcgcgtcttt cgtagttatg gcgaaacgcg
     5041 acctggtaat aacgttcgcc cgcagctgga ttcggtcgtg cgtattgctt ccctcaccaa
     5101 actaatgacc agtgaaatgc tggtgaaatt gctcgaccag gggaccgtaa agttaaacga
     5161 tccgctaagt aaatatgctc cgccaggcgc acgcgtgccg acctacaatg ggacgcccat
     5221 taccctagtg aatctggcaa cccataccag cgccctgccc cgtgaacagc ccggtggcgc
     5281 ggcacatcgt ccggtgtttg tctggccaac gcgcgagcaa cgctggaaat acctttctac
     5341 ggcgaagctg aaagccgcgc caggcagtca ggcggcgtac tctaaccttg cgttcgacct
     5401 gctggcagat gcactggcga atgcgtccgg caagccttat acccagttgt ttgaagagca
     5461 gattacccgt ccattgggga tgaaagacac cacctacacc ccctcaccgg atcagtgccg
     5521 ccgtttaatg gttgctgagc gcggtgccag tccgtgcaat aacacgctgg cggcaattgg
     5581 cagcggcggc gtctattcca cgcctggcga tatgatgcgc tggatgcagc agtatttgtc
     5641 atctgatttt tatcagcgca gtaaccaggc agatcgcatg cagacgttga tttatcagcg
     5701 cgcgcagttt acgaaagtga ttggcatgga tgtccccggc aaagcggatg cgcttggttt
     5761 aggctgggta tatatggccc cgaaagaggg tcgtccgggg attattcaga agacaggtgg
     5821 tggtggcggt ttcattacct atatggcgat gatcccgcag aagaatattg gcgcgtttgt
     5881 ggtggtcact cgttcgccgt tgacgcgctt taaaaatatg agcgatggca tcaatgacct
     5941 ggtgaccgag ctaagcggga ataaaccgct ggttatcccc gcatcctgat taatactctg
     6001 aaggcaaacg gttgatagcg accagtttgc ctttattcat ttcgatatag ccgcctttac
     6061 gcaaagcagc gagaacttct gcgacgacag aacgcgatat gcgtgtacgt tgatggatat
     6121 aattcatcac accaatacgt gatcgcaatt cttcattcca gtcaatcatt gataataatg
     6181 ttgcgcggat ttgttcgtag gaattatgcc caatgagctg cacgtcacgt aattccagaa
     6241 ttctattttg ccaggctaac cagtaaaaag cgtctcgcca gagttgattt tgttcaataa
     6301 gcgtaatggt ttgtttggct ggtagatgat atcccgtaca atttccttct gatattaatt
     6361 tgtatggtat atcgtttttc attaaaccat cagccagccc cataatataa ggagcctggg
     6421 taataccgat aagtacgttt tcttctctac gcagagagat aactccctct aaaataacga
     6481 aagtatcttc gttattgaca tcactggaaa atataacttg cttttcatta ttaaactcga
     6541 agcgcgtacc gtatctggac aaacatttat cgagcttacc aaattcctga agaggtttaa
     6601 ctacagataa catttgcgcg tcctttgcag taatgcccgt caaatccttg acgggcatta
     6661 tttagattaa attaccagaa tttcttcgga gtgaagaata ttaccaggta tatttaacac
     6721 ccacgttcgc ggaccagtct tgatctacgt caccaccacc gaggtagtta gcatcggtat
     6781 aggcgctgaa gttcttggtg aagctgaact gagtacccag accaacacgt accgcagacc
     6841 cttcagtacc gttatcgatg gaatcaccgt tcacatcgtt atcgttgtta gagtcgtcgt
     6901 agacgtaagc cagtttgaag tacggagtca gagcctggtc ttcgctgtag gtgaaggtat
     6961 aacctgcatc tacacccagt tcataacgca tgctgtcgta agactgaccg tcaactttca
     7021 tgtcgttgct cagctggtag tcatcaccag actggaacag accagaaata ctgccgtaag
     7081 gagtcacata accagcatca cccagtttga agtcgtaacc ggctttcaag ccgaagcccc
     7141 aagcgtcgga gttggtgcta ccgtcaacgt aagtaccgtt gctcatggtt gcagacaggt
     7201 cgttgttgaa gtgagagtag ctcaagctac catcaacaaa gacgttgttc gcgaagtgag
     7261 cagaagagta gatgtaggca gtctggctat cttgatctac ctgaccagaa cgatcattca
     7321 tgtcaccttt agcgaagcct gcagccgcac cgacgatcca cttagcgttg ttaccgtcaa
     7381 ttttggtatc aacaccgacc atgatgccgt taacatcctg atcatagttg atggtgccgt
     7441 tgtcgccgtt gaagttacca ccgaagtagc ttacccatgc gccgccgtta tcagccaggc
     7501 catgacgaga gttggtcaga cgagtaccaa cggtgtcttg ttccaggttc cagatattgg
     7561 tgttcgcaga cgggatgctc agcgccatgt tagcgtagtc ggtcagctcc atctgttgca
     7621 gaacaacggt gttaccgcgc tgttcagcct gataggtgta tgcacccagg tcagctttgt
     7681 tagcagcaga gaaggtcgcg ttgctgtttt tgtcgttgac gtagatcagc tcgttgccgt
     7741 tgtagtcagc gatagaacca gcaccagttg cgttgtcgat acgaactttg tagttaccgg
     7801 ttgcagctgc aacatggtca ccgttgttat caacttcgtt ctgaccagct tcgatagaag
     7861 cagtgttgtc gttatcgccg ttaccgttaa tggtcaggtg accatcagag ttcattgcga
     7921 ttaagccata accgtagttt gacttggtag tatcgttggt acggtcgttg gtcaggtctg
     7981 catcaagtac gaagcggttg ctttcgatgt tgaacacacc agcgtgaatg ttaccactgg
     8041 tatcagtagt gctggtcagg ttcagagtat caacctgcag agcattcgcc gcagtgttac
     8101 ctacatccag aataccgtcg ttagtcactt tgatgttgtt ggcatacaga gcagcatcac
     8161 ctacagccca accagcagaa acttcatcag aaatggttac ggtactgttg tcgacagtca
     8221 gtgaatcagt tgcaacatga ccatcttcgt tgatgttcag cgcagaaaga cctgtcagag
     8281 agatagagtc agaaatcagc gttgaatcag caacattaac agttgaacca ctgttgatgc
     8341 tcagagtatc aatcagagaa gttttggtgg tatcccactg tgaaccggta ttcagggtca
     8401 cattgaacag accgctctgg taaacctgat taccagtaat atgatcgaat tcactgtaac
     8461 ttgtatcatc gttttcaaca ccaacggttg ataacggcca caggctatta tcctcaatat
     8521 caatcagagt tgcagttgcc tcggtgccaa caccgtatgt agcgatgtca cctttaccat
     8581 ctccatcaac atcaatagaa ccagtttgat gaacagattg tgctgcccct acccatttac
     8641 taccgttatt taatgtcaga tccaggcgat ctgttccatc ccaaccgttg gtgtctactt
     8701 caccatcagc atcgcgataa ctatctgcac cacgtgggaa gaagttttca tcaaagttgc
     8761 tagagaagat tacatcgccc atcaaagtag agttactaaa gtcagcggtg gtctgcatag
     8821 cattatcagc attcggatgt gcaactacag ctaatgcaac atcatcccag cttgcaatag
     8881 tgctatcagc aacaccatct ccgtcagtat ctacggtgac aaagttagat ttaccactat
     8941 aatcgctggc attacctgta ttaccgaacc aaccagtagt accttcatca gtccatgaac
     9001 cagaggttac ggtagaatct ttaacagtga tggtgttatt aaacacttcg ctgctgttta
     9061 cgccagtgct gacagtgttg tcgtcaatat cttcccactc ataaccctga gtcaacgtaa
     9121 tacccgcaac gtgagagtta ttagtgatgg acagatcaac ttcctgatcc agagtaatag
     9181 ccgtaccaat gttgtaaacg tctacaacat gagtatcagc ggcattattg taagtgccgt
     9241 tatatgtgta gtgttcgtag ttgtcgtcga tcgttgaatt atcaacagtc agcgccagac
     9301 ggtcatggta gtaatcatcc gcacgatcag cacaatcagt tgtcatgcac tcggaataaa
     9361 tcataccgtg aatagtgctg tttttgattg tcagactatt ggcgttggta ttggtagaaa
     9421 taccgtcgtc cagatagtag gtagaaatca cgccgttaac ggtagcgtta ttaatgaccg
     9481 ggtagatatc gccgttgtaa tagctatcag cggcatagtt gttccagcct acataaccat
     9541 catagtaaac gtcatcagca taggtggcgt cgttgtagtg atggaaagta ttgtaagttt
     9601 gaccagaaat atcgttggtc gctgcattag cctgagaggt gatagccaga gtgcaagcca
     9661 atgctaattg tgatactaca agtttctttt tccaggagtg catttgtcat ccctcctcag
     9721 ggacgtaata agttgatcca tcaattatca atgcatagaa aacatattat gtttcgcgct
     9781 aacgattatg ctgtgttaca gacgaatggt tcaattattc tttcccctaa gtccgaatgt
     9841 ggacatatat aatttatgga gaaaaacacg catcgaattg ataaaaacac catatttcaa
     9901 tatgttgcag atgatgttat ttttgataca tacgcgtcac aaatatcaca gacacaaacc
     9961 agataaatat cctatcaata tgcaacaaag ttttaattat tcaatttata gcagggcaac
    10021 caaactccca aaataataac caagagctat taataaagat taaaaatatt ttcagtgaat
    10081 atgaaattat tgctatttct attacaacga tgtcctggtc tctctttcat tctcttcttg
    10141 tcaacatcgc gacaactttc gtaaaacact cctgccccgc ttcaggtata ctgtgcacct
    10201 taattccaca aacatcaggc agaccatgac agacttaatc caacgccctc gtcgcctgcg
    10261 caaatctcct gcgctgcgcg ctatgtttga agagacaaca cttagcctta acgacctggt
    10321 gttgccgatc tttgttgaag aagaaattga cgactacaaa gccgttgaag ccatgccagg
    10381 cgtgatgcgc attccagaga aacatctggc acgcgaaatt gaacgcatcg ccaacgccgg
    10441 tattcgttcc gtgatgactt tcggcatctc tcaccatacc gatgaaaccg gcagcgatgc
    10501 ctggcgggaa gatggactgg tggcgcgaat gtcgcgcatc tgcaagcaga ccgtgccaga
    10561 aatgatcgtc atgtcagaca cctgcttctg cgaatacaca tctcacggtc actgcggtgt
    10621 gctgtgcgag catggcgtcg acaacgacgc gactctggaa aatttaggca agcaagccgt
    10681 ggttgcagct gctgcaggcg cagacttcat cgccccttct gccgcgatgg acggccaggt
    10741 acaggcgatt cgccaggcgc tggacgctgc gggctttaaa gatacggcga ttatgtcgta
    10801 ttcgaccaag ttcgcctctt ccttttatgg tccgttccgt gaagctgccg gaagcgcatt
    10861 aaaaggcgac cgcaaaagct atcagatgaa cccaatgaac cgtcgtgagg cgattcgtga
    10921 gtcactgctg gatgaagccc agggcgcaga ctgtctgatg gttaaacctg ccggagcgta
    10981 cctcgacatc gtgcgtgagc tgcgtgaacg tactgaattg ccgattggcg cgtatcaggt
    11041 gagcggtgag tacgcgatga ttaagttcgc cgcgctggcg ggtgctatag atgaagagaa
    11101 agtcgtgctc gaaagcttag gttcaattaa gcgtgcgggt gcggatctga ttttcagcta
    11161 ctttgcgctg gatttggctg agaagaagat tctgcgttaa ttttgtttcc tctcagaaag
    11221 ggggagttaa cctgttgaaa gtacggtgaa cgccccctca ccctaaccct ctccccaagg
    11281 gggcgaaggg actgtccgtg ctcgcttcat ctatatcacc gttctttcgc cgctcgaggt
    11341 gaccgtccac tctcgtatca ccccgcccga taaaacggtt tatccccaag aatcgtcgcc
    11401 cgatgcataa tccgtcgctg tggcaggtaa tcggcattgg catagtgctg ggtcacgcgg
    11461 ttatcccaaa tcgcaatatc atttggttgc cagcgccagc gcacctgaaa ctccggttta
    11521 gtgatatgag cgaataaaaa acttaacaag gcttcgctct ctttctcgct cacatcaaca
    11581 attcgcgtag taaagccttc attcacaaac agcgcctgtt taccgctcac cggatgcgtt
    11641 cgcaccaccg gatgtagcaa cggcggattt ttcgcgaccg cctcgcgcca gcgttgatgt
    11701 tcctcctcgg ttttgcggta tttgtattcc gggaacgatt tacggaaatc atgctccgca
    11761 cgcagcccgc tcagcagctg gcggaaggga acagagagcg cctcataggc cgcaataccg
    11821 ctggtccaga gcgtatcacc gccggtcgaa ggtaactctt tagctgccag aatcgcccct
    11881 gcgggtggcg tttcaataaa tgtcacatcg gtatgccagt tgtcgttatc cggcggatta
    11941 tcgttatggg tatccagcac gatgatctcg tcaacccctt cggcatgagg gtaaacaggg
    12001 tgaatatgca gttcgccaaa acgctgggcc agcgcgcgtt gctgctgcgg cgtaatagtt
    12061 tgatcgcgca gaaacaccac ctgatggcgc agcaccgcat ggtaaagctg ttcaaactga
    12121 ttatcgctta acgggcgcgt caggtcggca cccgaaattt gtgcgccgat atacggcccc
    12181 agcggggtaa tgctcagacg ttcactcatt gtacttctcc atgccagggc gtcagacggc
    12241 gctgtaacgc gcgcaaaccc agttctaaaa gaaaggcgat aatcgcaatc accgcgatcc
    12301 ccgccagcac cacgtcagtc gcgagaaatt cacccgctga ctgaaccata aatcctaaac
    12361 cgcgcgtcgc ggcaatcagc tccgccgcca ccagcgtaga ccagcccaca cccagaccaa
    12421 tgcgtaatcc ggtgaggatt tccggcagcg cgccgggcaa aatgacaaac cacagcacct
    12481 gcgcacggct ggcacccagc gactgggcgg cacgaatgcg aacctgctgc gcgcttttca
    12541 cccccgccag cgccgacatc gccaccggtg caaaaatcgc taaatagatc agtaagatct
    12601 tcgaggtttc accaatacca aaccagatca ccatcagcgg caaataagcc agcggcggca
    12661 ccggacgata aagttcgatt accggatcca gaatgccgcg caccgtcggg cttagcccca
    12721 tggcgatccc gaccggaata ccgatcacca ccgccgcgag tagcgccagc acaatgcgcg
    12781 tcaggctggc tgccagatgc tgccacagcg tggcgtccat aaatccctgc ggtccggcaa
    12841 tggtgagcag ttttgccagt acttgttgcg gtggcggcaa aaacagcggg ctaatcagtt
    12901 gcagcgccgc caccgtccac catacagtga gtaaaaccgc taacgtgcca atgcttaagg
    12961 tcacctgacg cgagagcggc cagcgccatt tcagccgccg cgaatgcagt ttttcattaa
    13021 tgagcacact catgagaacg cctcccgttg ctcaaatacg cggcttaaaa catattcgcg
    13081 catggcgatg aattgtggat cggacttgat gctgcggctc gactctcccg caacaaagcg
    13141 gcgagcaaag ttgagcggca gccgctccag cacacggcca gggccggatg aaagcagaac
    13201 cagttcagtc gccataaaca ccgcttcttc tatatcgtgg gtaatcaaca gcacctgctt
    13261 gcccgtctcc tgccagagtt tcagcagcag agtttgcatc tggtcgcggg tgaaggcgtc
    13321 cagcgcacca aacggttcgt cgagtaataa cagctgggga ttcgccgcca gcgcgcgggc
    13381 aatccccacc cgctgacgtt gaccaccgga aagctgccag atatagcgtt tttctgcgcc
    13441 ttccagcccc acttttttca gcatctggtg cgcgatttcc agtcgctgca ttttctctat
    13501 acctgccaat tgcaggccga acgccacgtt gttctggaca ttgcgccacg gtagcagccc
    13561 ttcattctga aaaaccaccc cgcgctctgc tcccggtccc tcaatacgct tacccgccag
    13621 ttgaatgctg ccatgctgat aaggcacaaa accggcaatc agattcagca gggtggtttt
    13681 accgcagccg gacggcccca gcaccaccag tagctcaccg ctttccagcg tcaggttgat
    13741 atcttccagt gccggtttgc cgccataatc ggcatagaga tgagagattt gcagcatctg
    13801 cgcctccttt tattgcacga agcgcgaggt aacgtactgg ctgtaatcat tcgctacagc
    13861 cgggaccttg ccctgctctt tcaaaaactg cgcggtgtcg atgattgctt tgttcaccgg
    13921 tccggtcagt tctgccgttt gttgctgcgg cgtcagatag gtatttccct tcaccagccc
    13981 cggaacgtca ccttccggca cgccgcttaa acgcgccagt ttgctgatgt tttccggctg
    14041 tttcagccac gcgtctgggt tagcaatgta cggttgctga gcatcgatgg cgcttttagc
    14101 gaacgctttc acgacctcag gatgtttctc ggcaaaatct ttgcgcacca cccagacgtc
    14161 cagtgtcggc gcgccccact gcccgacctg ttcagaatcg gtcagcactt tgccgtcttt
    14221 ttccagggcg ttaaccgccg gtgcccagac ataagcacca tcaatatctc cccgctgcca
    14281 tgcagcgata atcgcaggcg gctgcaggtt cacaatctcc acttgcccag gtttaatgcc
    14341 ccagtgtttc agcgccgcca gcaggctgta gtgggtggtg gagataaacg gtacggcgat
    14401 gcgtttgcca atcagatctt ccggtttgct gatagttttc tttaccacca gcgcttcgga
    14461 gttacccagt tttgacgcca gcaagaagac ttcaatcggc acctgttggc tggctgcaac
    14521 cgctaacggg ctggaaccga ggttgccgat ttgcacgtcg cctgaagcca gcgcccgcac
    14581 gatgctggct ccgctgtcaa acttacgcca gtccacggtt gctccgcttt ctttagcaaa
    14641 ggtgttgtcg gcctgagcca ctttcgccgg ttcggctgag gtttgatagg ccacggtgac
    14701 gttcaccgcc tgtgcctgaa aagcgatgaa tgccagtgcg gcaagaagtg tgttacgcga
    14761 tgaaattgcc atgattgtct gctcccctgt cttgttatgg gagcagtatt caggaataaa
    14821 aacattcatt aaaagaatta gtcgttatcg cacagatgat tttattctta gcaaaaaaac
    14881 ggcaaaggaa agattaaata tgcaattact ccgttctaac aattgggaaa attactacac
    14941 ttcattttag tgatgcataa acaa
//
LOCUS       DAJOSY010000100        14956 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJOSY010000100 DAJOSY010000000
VERSION     DAJOSY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.100"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            122..364
                     /locus_tag="OHN27_003405"
     CDS             122..364
                     /locus_tag="OHN27_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287785.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            479..1117
                     /locus_tag="OHN27_003406"
     CDS             479..1117
                     /locus_tag="OHN27_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCQ2287786.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWTRTMGWTEYKQVRYATARSVFKWNGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSVGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1114..1851
                     /locus_tag="OHN27_003407"
     CDS             1114..1851
                     /locus_tag="OHN27_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCQ2287787.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1851..3947
                     /locus_tag="OHN27_003408"
     CDS             1851..3947
                     /locus_tag="OHN27_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCQ2287788.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3994..4272)
                     /locus_tag="OHN27_003409"
     CDS             complement(3994..4272)
                     /locus_tag="OHN27_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287789.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4273..4416
                     /locus_tag="OHN27_003410"
     CDS             4273..4416
                     /locus_tag="OHN27_003410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287790.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4542..4952
                     /gene="psiE"
                     /locus_tag="OHN27_003411"
     CDS             4542..4952
                     /gene="psiE"
                     /locus_tag="OHN27_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCQ2287791.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4996..6471)
                     /gene="xylE"
                     /locus_tag="OHN27_003412"
     CDS             complement(4996..6471)
                     /gene="xylE"
                     /locus_tag="OHN27_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCQ2287792.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6843..7733)
                     /gene="malG"
                     /locus_tag="OHN27_003413"
     CDS             complement(6843..7733)
                     /gene="malG"
                     /locus_tag="OHN27_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCQ2287793.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7748..9292)
                     /gene="malF"
                     /locus_tag="OHN27_003414"
     CDS             complement(7748..9292)
                     /gene="malF"
                     /locus_tag="OHN27_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCQ2287794.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9446..10636)
                     /gene="malE"
                     /locus_tag="OHN27_003415"
     CDS             complement(9446..10636)
                     /gene="malE"
                     /locus_tag="OHN27_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCQ2287795.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11001..12116
                     /gene="malK"
                     /locus_tag="OHN27_003416"
     CDS             11001..12116
                     /gene="malK"
                     /locus_tag="OHN27_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCQ2287796.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12188..13528
                     /gene="lamB"
                     /locus_tag="OHN27_003417"
     CDS             12188..13528
                     /gene="lamB"
                     /locus_tag="OHN27_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCQ2287797.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNADNNANFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13771..14691
                     /gene="malM"
                     /locus_tag="OHN27_003418"
     CDS             13771..14691
                     /gene="malM"
                     /locus_tag="OHN27_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCQ2287798.1"
                     /translation="MKMNKSLIVLCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3690 a         3864 c         3937 g         3465 t
ORIGIN      
        1 tatcctaaaa caccatcgct aattccccgc gccgtaattc gcatgcttta gttgtgtata
       61 ctcgatcccg cccgaaatgt ttttgggtaa atctccattc attcaatgaa gggaaattgt
      121 tatgaaaaaa gttctgtatg gcatttttgc catatctgcg cttgcggcga cttctgcgtg
      181 ggctgcacct gtacaggtgg gcgaagcggc agggtcggca gcaacgtcgg tttcggcggg
      241 gagttcctcc gcgaccagcg tcagcaccgt aagctcggcg gtgggtgtcg cgctcgcggc
      301 aaccggtggc ggtgatggtt ctaataccgg gaccaccaca accacaacca ccagtaccca
      361 gtaataaagt atgtatcccc aaaataattc gagtcatcgc atctgtggct tgaaatatga
      421 aggggattaa ccataaccac actccggtgt ggttattctg cccctctgga gaagagtcgt
      481 gaagcgacct gcactcattc ttatctgcct gttattacag gcctgttcag ccacgactaa
      541 agagctgggc aattcactgt gggacagtct gttcggcacg ccaggcgtac agctgacgga
      601 cgatgatatt caaaatatgc cctacgccag ccagtacatg cagcttaatg gcgggccgca
      661 attgtttgtg gtgctggcct tcgctgaaga cggacaacaa aaatgggtca ctcaggatca
      721 agccactctc gtcacacaac atggtcgtct ggtgaagact ttgcttggcg gcgacaacct
      781 gattgaagtg aataaccttg ccgccgaccc gctgattaaa cccgcacaaa tcattgatgg
      841 tgcaacctgg acgcgcacga tgggctggac cgagtataag caggtacgct acgccaccgc
      901 acgatcagtc ttcaaatgga atggtaccga taccgtcaaa gtcggcagcg atgaaacccc
      961 ggttcgcgtg ctggacgaag aagtctccac cgaccaggcg cgctggcata accgctattg
     1021 gatcgacagc gtagggcaaa ttcgccagtc ggaacagtat ctcggcgcgg attatttccc
     1081 ggtgaaaacc actctcatca aggcggcaaa acaatgatta aacaaacttt tgtcgcgttg
     1141 cttttgagtg tgggagcgtc atcggtcttc gcggcaggaa ccgtcaaggt gttcagcaat
     1201 ggcagcagtg aggccaaaac gctaacgggc gcagagcatt taatcgatct ggtaggccaa
     1261 ccgcggctgg caaacagctg gtggcccggt gcggtgatta gcgaagagct ggcaacggcg
     1321 gcagcattgc ggcagcagca ggcgttgctg acacggctgg cagaactggc tgcagattcc
     1381 agcgccgacg atgccgccgc gattaacgcc ttacgccagc aaattcaggc gttgaaagtg
     1441 acgggcaggc aaaaaatcaa tcttgatcct gatatcgtcc gcgttgccga acgcggtaac
     1501 ccaccgttgc agggcaacta cacgctgtgg gtcgggccac caccgtccac ggtcacgctg
     1561 ttcgggctta tcagccgtcc tggcaagcag ccattcactc caggtcgcga cgtggcgagc
     1621 tatctctccg ggcagaacct gcttagcggt gcggatcgca gctacgcgtg ggtggtttac
     1681 ccggacggac gcacgcaaaa agcgccggtg gcttactgga acaagcgtca cgtagagccg
     1741 atgcccggca gcattattta tgttggcctc gcggactccg tctggagtga gacgcctgat
     1801 gcccttaacg ccgacattct tcagactctg acgcagcgga taccccaata atgaaaaaaa
     1861 gacatctgct tagcttactg gcgctgggca ttagcatggc ttgctacggc gaaacatatc
     1921 ctgcgcccat tggcccgtcg cagtcggatt tcggtggcgt aggattatta caaacgccca
     1981 ccgcacgcat ggcgcgggaa ggggagttga gcctgaacta tcgcgataac gatcagtacc
     2041 gttattactc agcttcagtg caactcttcc cgtggctgga aacaacgctg cgctacaccg
     2101 acgtgcgcac ccggcagtac agcagcgtcg aagcgttctc tggcgatcaa acgtataaag
     2161 ataaagcctt cgatctcaaa ctgcgtttgt gggaagagag ttactggctg ccgcaagtgg
     2221 cggttggtgc gcgggatatc ggcggtacgg ggctgtttga tgcggaatat cttgttgcca
     2281 gcaaagcctg ggggccgttc gattttacgc tcggcctggg ctggggatat ttgggcacca
     2341 gcggtaatgt gaaaaatccg ctctgttcag ccagtgataa atattgctat cgcgataaca
     2401 gctacaaaca ggcgggatct atcgacggta gccagatgtt ccacggtcct gcctcactgt
     2461 ttggcggcgt ggaataccag acgccctggc aaccgctgcg cctgaaactg gagtatgaag
     2521 gcaataatta tcagcaggat tttgccggga agctggagca aaaaagtaag tttaacgtcg
     2581 gtgcgattta tcgcgttacc gattgggccg acgttaacct tagctatgaa cgtggcaaca
     2641 cctttatgtt tggcgttacg ttgcgcacca actttaacga tctgcgcccg tcttacaacg
     2701 ataacgcccg cccgcaatat caaccgcagc cgcaggatgc cattttgcag cattcggtgg
     2761 tggcgaatca gttaacgctg ttgaaataca atgctggact tgccgatcca cagatccagg
     2821 cgaaaggcga tacgctgtat gttaccggcg agcaggtgaa atatcgtgat tcgcgcgaag
     2881 ggatcatccg tgcgaatcgg atcgtgatga acgatctgcc ggatgggatc aaaacgatcc
     2941 gcattacgga aaatcgcctt aacatgccgc aggtgacgac ggaaaccgat gtcgccagcc
     3001 tgaaaaatca tctcgccgga gagccgttgg gccacgaaac gacgctggcg caaaaacgcg
     3061 tcgagccagt ggttccgcag tccaccgagc agggctggta tatcgacaaa tcacgctttg
     3121 atttccatat cgatccggtg ctgaaccagt cggtcggtgg cccggaaaac ttttacatgt
     3181 atcagctggg cgtgatggga acggcagatt tgtggctgac ggaccatctg ctgaccaccg
     3241 gcagcctgtt tgcaaatctt gccaacaact acgacaagtt taactacact aatcctccgc
     3301 aggactcgca cttaccgcgc gtgcgtaccc atgtgcgcga gtatgtgcag aacgatgtct
     3361 atgtgaataa cctgcaagcc aactacttcc agcatctggg caacggcttc tacggtcagg
     3421 tctacggtgg ttatctcgaa accatgtttg gcggtgcggg ggcagaagtg ttgtatcgcc
     3481 cgctggacag caactgggcg tttggtctgg atgccaacta cgttaaacag cgcgactggc
     3541 gtagtgcaaa agatatgatg aaattcaccg actacagcgt gaaaaccgga catctgaccg
     3601 cctactggac gccatctttc gctcaggatg tgttagttaa agccagcgtc gggcagtatc
     3661 tggcagggga taaaggcggc acgctggaga tcgccaaacg ctttgatagc ggcgtggtgg
     3721 tgggtggcta tgccacgatc actaatgttt cgaaagagga gtacggcgaa ggggacttca
     3781 ccaaaggcgt gtatgtctcg gtaccgttgg atctcttctc gtctggcccg acacgcagcc
     3841 gtgcggcgat tggctggacg ccgctgacgc gtgacggtgg tcagcaactt gggcgtaagt
     3901 tccagttgta tgacatgacc agcgaccgta gcgtcaattt ccgctaagtc atgggaaagg
     3961 tgccagtttt cgcactcact ggcaccttca ttcttaatgg cctgtcgagc catcaggaac
     4021 gcagttcact cccggtttgc gatctggtgt gtaaaggcga aaacattctg tcttcctgtc
     4081 cgtcgatatc ggttgcaggt ctggttgcct gagcgactgg gccatcattg ggctggagac
     4141 attgctttgc ggtaggtcgg ctttatcagc agtaccgagc ggaccagcat aagcaggaag
     4201 aacagagact gataacatca aagcagcaaa ataaggcttc atttttacca cctttatcag
     4261 gttacgtttc atttgttcca gaggaacatt gtcgattttt cgcgcattgc tggtggctgg
     4321 gaatcacctg aatgggtgat ttttgaatta ccggctttgg tgcggtttgt cttgccggat
     4381 gcgccgccag gcgcggctta tccggcctac gggtaggtat atccggcttt ggtggaggcg
     4441 cgctccaaat ccaggttgaa caaaacatac acaaaaaata tagatctccg tcacattttt
     4501 gcgttataca ggaagctcgc cactgtgaag gaggtactgc tatgacgtca ctctctcgtc
     4561 cgcgcgtgga gtttatctcc accattttgc agaccgtact caatcttggc ctgctgtgcc
     4621 tcggcctgat tttggttgtc ttcctcggca aagaaacggt gcatctggct gatgtgctgt
     4681 tcgcgccaga acaaaccagc aaatatgagc tggtagaagg actggtggtt tactttctct
     4741 atttcgaatt tatcgcgctg attgtgaagt actttcagtc cggttttcac ttcccgctac
     4801 gttactttgt ctatattggg atcaccgcca ttgtgcgctt gatcatcgtc gatcataaat
     4861 cgccactgga tgtgttgatc tactcggctg cgatcctgtt actggtgatc accctgtggc
     4921 tgtgtaattc gaaacggctg aagcgggagt aaaaagtcag cacgccgaaa tggcgcggcg
     4981 tgctggacag gaagattaca gcgtagcagt ttgttgtgtt ttcttcgttt ccggttccca
     5041 gagcgcttcc agctcctcaa gggttttacc tttggtttcc gggacaaatt tccacataaa
     5101 cagtgctgcc agaacgccca tacaaccgta aatccagtag gagaaaccgt tgtggaaatg
     5161 ggccaccagc caggagtttt tgtccatcat cgggaaggtc caggagacga agtagttcgc
     5221 cagccactgg gccgccaccg cgattgccag cgctttacca cgaatagcat tcgggaagat
     5281 ttccgacagc agtacccagc ataccggacc ccaggacatg gcaaaggcgg caacatagaa
     5341 cagcatcgac agtagcgcca caatacccgg tgcctgagtg taaaacgcgg taccgaggct
     5401 aaacataccg attgccattc cgagtgcgcc gataatttgc agtggcttac gaccaaattt
     5461 atccaccgtc ataattgcca gaacggtgaa ggtgaggttg ataactccga caataatggt
     5521 ctgcaacagc gcgatatccg tgctggcccc cagcgttttg aacacttccg gcgcgtagta
     5581 cagcaccaca ttgatgccga caaattgctg gaagatggag agcattacgc cgattacaat
     5641 cacgcccacg ccaaacatca gcagacgacc accggttttg cggccatgat ccagggagtg
     5701 tttaatttcc tgtactgcct gagttgcaag cgtgttgccc ataattttgc gcaggatacc
     5761 ttccgcctgt tcttgcttgc cgcgcgacat cagccagcga ggactttctg gcacggtata
     5821 cagcagcatt aagaacagca gtgcagggat acattccgag gcaaacatat aacgccagcc
     5881 gtcagtattc agccagctgg catcaccgga acgggcaata aaatagttta cgcagtaaac
     5941 taaaagttgc ccgaaaataa tcgcaaactg gttaaaagag accagtttcc cgcgaatatg
     6001 agctggagcc agttccgcaa tatacattgg cgagagcatt gaggctaaac caacgccaat
     6061 accgccaata atgcgataaa taacaaattc cgggacataa cctgccagat aaacaggcac
     6121 tgtgttgtcc gggtttatag aggtaaaacc aagttctggc caggcagaac ctacaccaga
     6181 aataaaaaac aggacagcag caatcttaag tgaatcacga cgaccgaagc ggttactgca
     6241 ataaccaccg agggcaccgc cgatgatgca accaatcaga gcgctggcca cgcaaaaccc
     6301 taacagggag ttggcagcgg attcacttaa gttttgtgga gcaacaaaga cggtattgag
     6361 tgactcaaca gtaccggaaa taacggcggt gtcgtagcca aataataaac cacctaatgt
     6421 agcgactaag gtaatcgaaa atatataact ggaattatac tgggtattca ttcagacctg
     6481 ccttagacca ttctgatctt ttatcagtgc attgatgttg gaattgtcag gcagttaagg
     6541 caattatcat tttttacaat gcgtttacgt aatgtctttt ctgtgatctt aattgtgata
     6601 attatccaaa attgataaaa acaatactat tgccgtgact cagagcacga aagagaatta
     6661 tcgtaagtgg gaaaacaaat aacgtaaaaa taataagctc tatgatggaa atattaaccg
     6721 gcgaacgatt cagattgcag acgaaagaaa aaaaggcgct ccgtggagcg ccgaataaca
     6781 gtcacaagtt gggataacgt aagttgaggg tgcagcggca taacattggc agaacaacat
     6841 ctttaacctt tcacaccacc tgccgtcagg ccgttgacca gccagcgttg agccagcaag
     6901 aagacgatgg tgatcggtaa tgcagacatc acggcagcgg cggcaaagtc accccacagg
     6961 tagttttgcg ggttgaggta ttgctgcatc cccacggcca gggtgtagct gtttacgtca
     7021 cgcagtaaca gcgacgcgac cggaacttca gtaatggcag cgataaacga caggatgaat
     7081 accaccgcca gaatcggtac tgacagcggc aacaggacaa ggcggaaggc ctgccacggt
     7141 gtcgcaccat ccagcgcagc agcttcttcc agcgaactgt cgatggtttc gaaatagcct
     7201 ttgatggtcc agacatgcag cgcaatccca cccagatacg cgaaaattac gccgccgtga
     7261 gtattcaggc caatgaatgg aatgtactca cccagacgat caaacaacgc atacaacgcg
     7321 accagtgaaa gtactgccgg gaacatctgg aaaatcagca ttcctttcag cagcgtcgct
     7381 ttgcctggaa agcgcatacg ggcgaaagcg taggcgcagg tggtggagag cgccacaatg
     7441 ccaatcgcgg aaatcccggc gacctttacc gagttccaca gccacagcag taccgggaat
     7501 ggcggtggcg taatgcgacc atcagcctgt tcaacgctaa aacctaacgc cagtttccag
     7561 tgatcccagg agatttgctc cgggatcagg ctgccggtcg caaagtttcc ctgacgcagc
     7621 gagatagcga cgaccatcag cagcgggaac ataatcgctg cgataaaaag tagcagtagc
     7681 aggtgagtaa taaataaacg agctttttgc gatttcggtt ggaccattgc catttttgtt
     7741 atctccctta atcaaacttc attcgcgtgg ctttcaggtt cactatcgcc agcgcaccca
     7801 ccagcaggaa gatcagcgtg gcaattgctg ccgccagacc gaagtcctga cccccgccgc
     7861 cttcaaaagc gatgcggtag gtgtagttaa caagcaggtc ggtataaccg gctggcgtgg
     7921 tcgtgccaag acgatccggg ccgccgttgg ttaacagttg aatcagcacg aagttgttaa
     7981 agttaaaggc gaagctggcg atcatcagcg gcgtcagcgg tttaatcagc agcggcagcg
     8041 taatcttaaa gaagttctgg aacggacctg cgccatccat tgctgaggct tcatacaaat
     8101 cgtccggaat cgctttcagc aagcccatgc agaggatcat catgtacgga taacccagcc
     8161 aggtattgac gataattagc atcgtgcggg cggtggtcgg atcgctgaac caggcgggct
     8221 tcacgccaaa cagcgcgctc aacatcatgt tgatttcacc gaagctctgg ttaaacaacc
     8281 ctttgaaaat caagattgaa atgaacgatg gcaccgcgta gggcagaatc agcaggacgc
     8341 gatagaccgc tttgccgcgc aacgcttccc actgcaccag acacgccaga accatgccga
     8401 ccgccaccgt taaaaagaca gtgatcagcg agaacaccac ggtccagacg aaaatggcga
     8461 ggaacggttt ctgaatgcct tcgtcggtaa agacgcgggt aaagtttttc cagccggtgg
     8521 tcacggtgta accggggctt agcttttcat caccccagtt gccgtcggcg gtaatggact
     8581 ggtaaaagcc aatttggtta ttcggacgat atttcacgcc gctctgatta ttcgtcaacg
     8641 tgccgtcacc gtcgagtgtg tagagcggct gcgtgccaga aaactggcgc agggagctca
     8701 tcatcacttt gttgccatcc ggcagaatgg cggtaatgtc actcagcgcc tgacgattct
     8761 gggtaatcac gcgcagattc gcgcgttcgc cttcgggctg ggcggtcgtt tctttcagtt
     8821 gcagtttttg ctcgccgcca aatttaaaag cgtcggagag gtaatttttg ccggtttcgc
     8881 cgtcgctgag cgccagttgc cactcatcgc ccgccgggta aagaccaaag ttataggttt
     8941 tgcctgcttg ccaggagcga tctaacaaca cttcctgcgc acgttcaaaa gtcagctggt
     9001 tagtgctgct gtagttggtg aaggcaatgg cgatggtgca gaccagaggg aagaggacga
     9061 ataatcccat tccagccatt cccgggtaaa catagcgcca ggcgtaggct ttacgattgg
     9121 cgaaaatata cagccccgct gaactcaata tcagcgtggt aatggcgaac aggtattccc
     9181 cttgtgcgta cattaaaaca acaaggtaac ccaccagcag gccgagcaga cctagcactg
     9241 accatttcag cgcgtcgctt tgccaccaat gtttcttttt aatgacatcc atggggttct
     9301 tcctcattcc aggacggata aggctttcac gccttatccg acaacaactg cctgatgcga
     9361 cgctgacgcg tcttatcagg cctacatacg tttcggtttt gtaggccgga caaggcgttc
     9421 acgccgcatc cggcatttca cagcattact tggtgatacg agtctgcgcg tctttcaggg
     9481 cttcatcgac agtctgacga ccgctggcgg cgttgatcac cgcagtacgc acggcatacc
     9541 agaaagcgga catctgcggg atgttcggca tgatttcacc tttctgggcg ttttccatgg
     9601 tggcggcaat acgtggatct ttcgccaact cttcctcgta agacttcagc gctacggcac
     9661 ccagcggttt gtctttatta accgcttcca gaccttcatc agtcagcaga tagttttcga
     9721 ggaactcttt cgccagctct ttgttcggac tggcggcgtt aatacctgcg ctcagcacgc
     9781 caacgaacgg tttggatggt tgacccttga aggtcggcag taccgttaca ccataattca
     9841 ctttgctggt gtcgatgttg gaccatgccc acgggccgtt gatggtcatc gctgtttcgc
     9901 ctttattaaa ggcagcttct gcgatggagt aatcggtgtc tgcattcatg tgtttgtttt
     9961 taatcaggtc aaccaggaag gtcagacccg ctttcgcgcc agcgttatcc acgcccacgt
    10021 ctttaatgtc gtacttgccg ttttcatact tgaacgcata acccccgtca gcagcaatca
    10081 gcggccaggt gaagtacggt tcttgcaggt tgaacatcag cgcgctctta cctttcgctt
    10141 tcagttcttt atccagcgcc gggatctctt cccaggtttt tggcgggttc ggcagcagat
    10201 ctttgttata aatcagcgat aacgcttcaa cagcgatcgg gtaagcaatc agcttgccgt
    10261 tgtaacgtac ggcatcccag gtaaacggat acagcttgtc ctggaacgct ttgtccgggg
    10321 tgatttcagc caacaggcca gattgagcgt agccaccaaa gcggtcgtgt gcccagaaga
    10381 taatgtcagg gccatcgcca gttgccgcaa cctgtgggaa tttctcttcc agtttatccg
    10441 gatgctcaac ggtgacttta attccggtat ctttctcgaa tttcttaccg acttcagcga
    10501 gaccgttata gcctttatcg ccgttaatcc agattaccag tttaccttct tcgattttgg
    10561 cgagagccga ggcggaaaac atcatcgtcg ttaatgcgga taatgcgagg atgcgtgcac
    10621 ctgtttttat tttcataatc tatggtcctt gttggtgaag tgctcgtgaa aacacctaaa
    10681 cggactctag tttctttata cggcaacctc tttccatcct ccttgcccct acgccccacc
    10741 gtcgctttgt gtgatctctg ttacagaatt ggcggtaatg tggagatgcg cacataaaat
    10801 cgccacgatt tttgcaagca acatcacgaa attccttaca tgacctcggt ttagttcaca
    10861 gaagccgtgt tctcatcctc ccgcctcctc ccccataaaa aagccagggg gtggaggatt
    10921 taagccatct cctgatgacg catagtcagc ccatcatgaa tgttgctgtc gatgacaggt
    10981 tgttacaaag ggagaagggc atggcgagcg tacagctgca aaatgtaacg aaagcctggg
    11041 gcgaggtcgt ggtatcgaaa gatatcaatc tcgatatcca tgaaggtgaa ttcgtggtgt
    11101 ttgtcggacc gtctggctgc ggtaaatcga ctttactgcg catgattgcc gggcttgaga
    11161 cgatcaccag cggcgacctg ttcatcggtg agaaacggat gaatgacact ccgccagcag
    11221 aacgcggcgt tggtatggtg tttcagtctt acgcgctcta tccccacctg tcagtagcag
    11281 aaaacatgtc atttggcctg aaactggctg gcgcaaaaaa agaggtgatt aaccaacgcg
    11341 ttaaccaggt ggcggaagtg ctacaactgg cgcatttgct ggatcgcaaa ccgaaagcgc
    11401 tctccggtgg tcagcgtcag cgtgtggcga ttggccgtac gctggtggcc gagccaagcg
    11461 tatttttgct cgatgaaccg ctctccaacc tcgatgctgc actgcgtgtg caaatgcgta
    11521 tcgaaatctc ccgtctgcat aaacgcctgg gccgcacaat gatttacgtc acccacgatc
    11581 aggtcgaagc gatgacgctg gccgacaaaa tcgtggtgct ggacgccggt cgcgtggcgc
    11641 aggttgggaa accgctggag ctgtaccact atccggcaga ccgttttgtc gccggattta
    11701 tcggttcgcc aaagatgaac ttcctgccgg taaaagtgac cgccaccgca atcgatcaag
    11761 tgcaggtgga gctgccgatg ccaaatcgtc agcaagtctg gctgccagtt gaaagccgtg
    11821 atgtccaggt tggagccaat atgtcgctgg gtattcgccc ggaacatcta ctgccgagtg
    11881 atatcgctga cgtcatcctt gagggtgaag ttcaggtcgt cgagcaactc ggcaacgaaa
    11941 ctcaaatcca tatccagatc ccttccattc gtcaaaacct ggtgtaccgc cagaacgacg
    12001 tggtgttggt agaagaaggt gccacattcg ctatcggcct gccgccagag cgttgccatc
    12061 tgttccgtga ggatggcact gcatgtcgtc gactgcataa ggagccgggc gtttaagcac
    12121 cccacaaaac acacaaagcc tgtcacaggt gatgtgaaaa aagaaaagca atgactcagg
    12181 agatagaatg atgattactc tgcgcaaact tcctctggcg gttgccgtcg cagcgggcgt
    12241 aatgtctgct caggcaatgg ctgttgattt ccacggctat gcacgttccg gtattggttg
    12301 gacaggtagc ggcggtgaac aacagtgttt ccagactacc ggtgctcaaa gtaaataccg
    12361 tcttggcaac gaatgtgaaa cttatgctga attaaaattg ggtcaggaag tgtggaaaga
    12421 gggcgataag agcttctatt tcgacactaa cgtggcctat tccgtcgcac aacagaatga
    12481 ctgggaagct accgatccgg ccttccgtga agcaaacgtg cagggtaaaa acctgatcga
    12541 atggctgcca ggctccacca tctgggcagg taagcgcttc taccaacgtc atgacgttca
    12601 tatgatcgac ttctactact gggatatttc tggtcctggt gccggtctgg aaaacatcga
    12661 tgttggcttc ggtaaactct ctctggcagc aacccgctcc tctgaagctg gtggttcttc
    12721 ctctttcgcc agcaacaata tttatgacta taccaacgaa accgcgaacg acgttttcga
    12781 tgtgcgttta gcgcagatgg aaatcaaccc gggcggcaca ttagaactgg gtgtcgacta
    12841 cggtcgtgcc aacttgcgtg ataactatcg tctggttgat ggcgcatcga aagacggctg
    12901 gttattcact gctgaacata ctcagagtgt cctgaagggc tttaacaagt ttgttgttca
    12961 gtacgctact gactcgatga cctcgcaggg taaagggctg tcgcagggtt ctggcgttgc
    13021 atttgataac gaaaaatttg cctacaatat caacaacaac ggtcacatgc tgcgtatcct
    13081 cgaccacggt gcgatctcca tgggcgacaa ctgggacatg atgtacgtgg gtatgtacca
    13141 ggatatcaac tgggataacg acaacggcac caagtggtgg accgtcggta ttcgcccgat
    13201 gtacaagtgg acgccaatca tgagcaccgt gatggaaatc ggctacgaca acgtcgaatc
    13261 ccagcgcacc ggcgataaga acaatcagta caaaattacc ctcgcacaac aatggcaggc
    13321 tggcgacagc atctggtcac gcccggctat tcgtgtcttc gcaacctacg ccaagtggga
    13381 tgagaaatgg ggttacgact acaccggtaa cgctgataac aacgcgaact tcggcaaagc
    13441 cgttcctgct gatttcaacg gcggcagctt cggtcgtggc gacagcgacg agtggacctt
    13501 cggtgcccag atggaaatct ggtggtaata gcaaaacctg ggccggataa ggcgtttacg
    13561 ccgcattcgg caaccaacgc ctgatgcgac gcttgcgcgt cttatcaggc ctacaacggc
    13621 tgtcaaatgt aggccggata aggcgtttac gccgcatccg gcataaaaac aggttgtcat
    13681 tatctgaaag gggcgaaagc ccctctgatt atcgggttta gcgcgctatt gcctggctac
    13741 cgctgagctc cagattttga ggtgaaaaca atgaaaatga ataaaagtct catcgtcctc
    13801 tgtttatcag cagggttact ggcaagcgcg cctggaatta gccttgccga tgttaactac
    13861 gtaccgcaaa acaccagcga cgcgccagcc attccatctg ctgcgctgca acaactcacc
    13921 tggacaccgg tcgatcaatc taaaacccag accacccaac tggcgaccgg cggccaacaa
    13981 ctgaacgttc ccggcatcag tggtccggtt gctgcgtaca gcgtcccggc aaacattggc
    14041 gaactgaccc tgacgctgac cagcgaagtg aacaaacaaa ccagcgtttt tgcgccgaac
    14101 gtgctgattc ttgatcagaa catgacccca tcagccttct tccccagcag ttatttcacc
    14161 taccaggaac caggcgtgat gagtgcagat cggctggaag gcgttatgcg cctgacaccg
    14221 gcgttggggc agcaaaaact ttatgttctg gtctttacca cggaaaaaga tctccagcag
    14281 acgacccaac tgctcgaccc ggctaaagcc tatgccaagg gcgtcggtaa ctcgatcccg
    14341 gatatccccg atccggttgc tcgtcatacc accgatggct tactgaaact gaaagtgaaa
    14401 acgaactcca gctccagcgt gttggtagga cctttatttg gttcttccgc tccagctccg
    14461 gttacggtag gtaacacggc ggcaccagct gtggctgcac ccgctccggc accggtgaag
    14521 aaaagcgagc cgatgctcaa cgacacggaa agttatttta ataccgcgat caaaaacgct
    14581 gtcgcgaaag gtgatgttga taaggcgtta aaactgcttg atgaagctga acgcctggga
    14641 tcgacatctg cccgttccac ctttatcagc agtgtaaaag gcaaggggta attacgcccc
    14701 acagtgctga ttttgcaaca actggtgcgt ctcctggcgc accttttttt atgcttcctt
    14761 cctgggatat gagcgatttt ttatagtaac tcacttcttc ttcactaaga atatccatta
    14821 tctcaatgcc ttatcagaga ttcttttcct ttcgccggta gtgtctggac attcaggcta
    14881 cttttccagg ttattttatt tctgttatgc agaggtttta tgataagtca tatcctaaat
    14941 tctggcggca ataact
//
LOCUS       DAJOSY010000101        14940 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJOSY010000101 DAJOSY010000000
VERSION     DAJOSY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.101"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..119)
                     /gene="yeaR"
                     /locus_tag="OHN27_003419"
     CDS             complement(<1..119)
                     /gene="yeaR"
                     /locus_tag="OHN27_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCQ2287799.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGV"
     gene            complement(292..930)
                     /gene="leuE"
                     /locus_tag="OHN27_003420"
     CDS             complement(292..930)
                     /gene="leuE"
                     /locus_tag="OHN27_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCQ2287800.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1057..1980)
                     /gene="dmlR"
                     /locus_tag="OHN27_003421"
     CDS             complement(1057..1980)
                     /gene="dmlR"
                     /locus_tag="OHN27_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCQ2287801.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGPHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2083..3168
                     /gene="dmlA"
                     /locus_tag="OHN27_003422"
     CDS             2083..3168
                     /gene="dmlA"
                     /locus_tag="OHN27_003422"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCQ2287802.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            3419..5029
                     /gene="yeaV"
                     /locus_tag="OHN27_003423"
     CDS             3419..5029
                     /gene="yeaV"
                     /locus_tag="OHN27_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCQ2287803.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5061..6185
                     /gene="yeaW"
                     /locus_tag="OHN27_003424"
     CDS             5061..6185
                     /gene="yeaW"
                     /locus_tag="OHN27_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCQ2287804.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            6241..7206
                     /gene="yeaX"
                     /locus_tag="OHN27_003425"
     CDS             6241..7206
                     /gene="yeaX"
                     /locus_tag="OHN27_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCQ2287805.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7260..8375)
                     /gene="rnd"
                     /locus_tag="OHN27_003426"
     CDS             complement(7260..8375)
                     /gene="rnd"
                     /locus_tag="OHN27_003426"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCQ2287806.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDMTNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(8457..10142)
                     /gene="fadD"
                     /locus_tag="OHN27_003427"
     CDS             complement(8457..10142)
                     /gene="fadD"
                     /locus_tag="OHN27_003427"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCQ2287807.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(10347..10928)
                     /gene="yeaY"
                     /locus_tag="OHN27_003428"
     CDS             complement(10347..10928)
                     /gene="yeaY"
                     /locus_tag="OHN27_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCQ2287808.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10968..11663)
                     /gene="tsaB"
                     /locus_tag="OHN27_003429"
     CDS             complement(10968..11663)
                     /gene="tsaB"
                     /locus_tag="OHN27_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCQ2287809.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(11721..13631)
                     /locus_tag="OHN27_003430"
     CDS             complement(11721..13631)
                     /locus_tag="OHN27_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCQ2287810.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLLTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13763..14107
                     /locus_tag="OHN27_003431"
     CDS             13763..14107
                     /locus_tag="OHN27_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCQ2287811.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            14469..14828
                     /locus_tag="OHN27_003432"
     CDS             14469..14828
                     /locus_tag="OHN27_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCQ2287812.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIK
                     NPEYFSTYMQEELKALV"
BASE COUNT         3692 a         3895 c         3665 g         3688 t
ORIGIN      
        1 taaacccccg ggcgcgttcc tttatcaaga tgacgttcga atattccggc aggtgcagtt
       61 tgtttattcc agaaaggcgt tgagcgcgta tgaatataat tctgtgggat ttgaagcatc
      121 cttttccctc cttcggtgaa tgcgctgaaa acggtttatt ccagccgttt cagggtacgc
      181 ctgataattt gcattttaaa taccatttat tggttacttt ttagcaccat atcagcgaag
      241 aatcagggag gattatagat gggaagcccg cgacaacgcg ggctgaaagc atcaggattg
      301 cagcgtcgcc agtcgggcag cgaaacccac gaacatcaaa ccaatcagtg agttgccaac
      361 tttagccagt ttctttttgg tacgtatgta ctgcgtgaca aaagcaccag atataatcag
      421 gaagctcaaa tagcagaaac tcaccagttc cagcgtcgtc gccagaataa agaatgaaat
      481 tcccgtatgt ggggcattaa catcgataaa ctgtacgaaa aacgacacat agaacaaaat
      541 ggctttcgga ttagtcaggc tcaaaattaa cgcgcgttta aaaatagcac cgtattgggg
      601 ctcatcggat ttggcctcgc tatttttacc cttcagggtc gcgtaaagaa ttttactccc
      661 cagatagagc aaataaaacg caccaagata acgtacaatg ttgaataata tcggggtggt
      721 cttaattaat gtcgccactc cagcccatgc cagaaacatc aataccgcat cgccaataaa
      781 tacaccgcag gccgcaagat aaccgccttt cataccgcta ctgacgctat ttttgagtac
      841 aaacagggta tttggccctg gcaccaacac aataaaaatg gccccaacca gataggtcca
      901 gtaattcaga accccgtatt cagcgaacac attaacctct ttaattatct ttcgatcatg
      961 cgcgattaaa ggtgaatatg ctaaccaatc tgtagcggct tagaaaggag aaaatcaggt
     1021 tttaacctga tatcaacccg ataattgaat cattaatcag gcatgctcca gtgaaaaatt
     1081 cgggtagtgc tcggcaaaat actggcgtaa aaattctacc gttatccgca ctttcgctga
     1141 cgtcgccagc cttgaaacat aaacggccca gacgttcgct ggctggtaat attccggtag
     1201 cacttgcact aaatgaccac tggcaatgtt ttcgctaaca tcccaccagg aacgcagagc
     1261 aatcccctgc ccatcgagac accactgatg gacaatttct ccatgattag acgacagtgg
     1321 gccggtgact ttaatcgcat gggggccttc tttattgcgt aattgccaga caccaaaagg
     1381 atgatcgcgc tctttaatga ccaggcacgg caatgcagaa agatcggtta aatgtttcgg
     1441 cgcgccatgt tgcgcaataa attccggcga cgcgcagaga atacggtaat tggtcgccag
     1501 tttgcgggcg attaaattag gggcgatgtc atcgccaatg cgaatatcga gatcgacacc
     1561 ttcattgacc aaatcgacca gtcggtcttc cacatcaaaa cgtaattcca gttgcggata
     1621 cgccttcgcc agcgctgata acgccggagc caccacctgt cgtccaaaac caaagctgct
     1681 gataatacgc agcatcccct gcggcacctg acgcacgtca gaaagttcgt ccatcatctg
     1741 accgacatcc tgcaaaatcc gctgcgccca ttcataaatc cgctctcctt cttcggtaat
     1801 ggtgacgcgg cgggtggtgc ggtgtaacaa caccacgttt agcgtttgct ccagcaaggc
     1861 gatgcgcttg ctgacgaacg ccggtgaaac gcccagttct tccgccacgg cggcaaaacc
     1921 agcccggcga gccaccagca taaagacgcg caaatcattc agcagcggta aattattcat
     1981 gattcgtgtt ttatgtttca ccagttacgg ggattaattc cttttcagtc aattatagga
     2041 tggtgatgtt gtcaattttg atggtcagga agtgagaacc caatgatgaa aacgatgcgt
     2101 attgctgcga tcccgggaga cgggattggc aaagaagtcc ttcctgaagg gattcgcgtg
     2161 ttacaggctg ccgctgagcg ctggggcttc gccttgagtt ttgagcaaat ggagtgggcg
     2221 agctgcgagt attacagcca tcacggtaaa atgatgccgg acgactggca tgagcaactt
     2281 agccgtttcg acgccatcta ttttggtgcc gtcggctggc cggataccgt tccggaccat
     2341 atttcgttgt ggggttcgct gctgaaattt cgtcgtgaat tcgaccagta cgtcaacctg
     2401 cgcccggttc gtctctttcc tggcgttccc tgcccgctgg cgggaaaaca gcctggcgac
     2461 atcgattttt acgtggtcag ggaaaacacc gaaggcgaat attcctcgct cggcggtaga
     2521 gtgaatgaag gtacagagca tgaagtcgtc attcaggaat cggtatttac gcgtcgtggt
     2581 gtcgatcgca ttttgcgtta tgccttcgaa cttgcgcaaa gccgcccacg taagacgcta
     2641 acttctgcca ctaaatcgaa cggtttagcc atcagcatgc cgtactggga tgagcgagtg
     2701 gaagcaatgg ccgagaatta cccggagatc cgctgggaca agcagcatat tgatattctc
     2761 tgcgcgcgtt ttgtgatgca gccggaacgc ttcgatgtgg tggtggcgtc caatttgttt
     2821 ggcgatatcc tttccgatct tggcccggcc tgcaccggca ccattggcat tgccccatcc
     2881 gccaacctga atccggaacg cactttcccg tcgctcttcg agcctgtcca cggttccgcg
     2941 ccggatatct acgggaaaaa tattgctaac cctatcgcca cgatttgggc cggggcaatg
     3001 atgctcgatt ttctcggcaa tggcgatgag cgtttccagc aagcgcataa cggtattctg
     3061 gcagcaattg aagaagtgat tgctcacggg ccgaaaacac ctgatatgaa aggcaatgcc
     3121 accacgccac aggttgccga cgcgatttgc aaaattattt tgcgttaagg tcaaaccagt
     3181 ttatttgaac cgcgtcactg acgcggtttt tttattcgtt ctttgcagta aataacctgc
     3241 gtcatttcac cttttattgt ttccgtttcg tgttttatgg ctttccgtat tcttaattgt
     3301 ttaatttatg taacatacaa ataatcaatt tgttaaacac aacatacaac tcattaccct
     3361 gcaaataatt acaactggca aaagccagag acgggctttt atgcccagga gatgactcat
     3421 gatgagcaat gttaagaaaa aagatgtgcc gctgataagc atcagcctgg tggccattct
     3481 tttcatcgca gctgcattaa gccttttccc acaacaatcg gccgacgcgg ccaacgcaat
     3541 atacactttt gttacgcgta cgttaggttc cgccgtacag gtattggttt tgctggcaat
     3601 gggactggtg atttatttag ccaccagtaa atacggcaat attcgtcttg gcgaaggaaa
     3661 accggaatac agcacgctct cctggctgtt tatgtttatt tgtgccggtt taggttcttc
     3721 tacgctttat tggggggttg ctgaatgggc ctattattat caaacacctg gattaaatat
     3781 cgcaccgcgt tcacaacagg cactcgaatt tagcgttccc tactctttct tccactgggg
     3841 catcagcgcc tgggcaactt atacgctggc ctcattaatc atggcttatc actttcatgt
     3901 gcggaaaaac aaaggtctga gcctttccgg cattattgct gctattaccg gcgttcgccc
     3961 gcaaggccca tggggaaaac tggtcgattt gatgttcctg atcgccactg tcggcgcact
     4021 gaccatttcc cttgttgtta ccgcagcaac ctttacccgt gggctttccg cgctgaccgg
     4081 tttacccgat aacttcaccg tgcaggcatt tgtgatcctg ctttccggcg gcattttttg
     4141 cctaagctcg tggattggta tcaacaacgg tttgcaacgt ctgagcaaaa tggttggctg
     4201 gggcgcgttc ctgctgccat tactggtgct gattgtcggc ccaaccgaat ttattaccaa
     4261 cagcatcatc aatgccatcg gcctgaccac gcaaaacttc ctgcaaatga gcttattcac
     4321 cgatccgctt ggcgatggtt catttacccg caactggacc gttttctact ggctgtggtg
     4381 gatctcatac acccctggcg tagcaatgtt tgtcacccgc gtttcccgcg gtcgtaagat
     4441 taaagaagtt atctggggac tgatcctcgg cagcaccgtc ggttgctggt tcttctttgg
     4501 cgtaatggaa agctatgcca ttcatcagtt tatcaatggc gtaatcaacg tcccacaggt
     4561 gctggaaaca ctgggcggcg aaacagctgt acagcaagtt ctgatgtcgt tgccagccgg
     4621 taaattgttc ctcgccgcat acctgggcgt gatgattatt ttccttgcct cgcatatgga
     4681 tgcagtggcc tacaccatgg cggcgaccag tacgcgtaat ctccaggaag gtgacgatcc
     4741 tgaccgtggg ctgcgtcttt tctggtgcgt ggtgatcact ctgatcccgc tttccatctt
     4801 gtttaccggt gcttcgctgg aaacgatgaa aaccaccgtc gtgctcacag cccttccctt
     4861 cctcgtcatt ttactggtga aagtcggcgg gtttattcgc tggctgaaac aggattacgc
     4921 cgacattccg gctcatcaag ttgaacatta tctcccgcag acaccggttg aagccctgga
     4981 aaaaacgcca gtgctccctg cgggaaccgt attcaaaggc gacaactgag cgccatcatc
     5041 ctaacgataa aggtatccct atgagcaatc tgagccctga ctttgtacta cccgaaaatt
     5101 tttgcgctaa cccgcaagag gcgtggacca ttcctgcccg tttttatacc gatcagaacg
     5161 cgtttgaaca cgaaaaagag aacgtcttcg ccaaaagctg gatttgcgtc gctcacagca
     5221 gcgaactggc gaatgccaat gattatgtga cgcgtgagat cattggcgaa agcatcgtgc
     5281 tggtacgcgg tcgtgataag gttttgcgcg cgttctataa cgtgtgtccg caccgtggtc
     5341 atcagttgtt gagcggtgaa ggaaaagcaa aaaatgtgat tacctgcccg tatcacgcat
     5401 gggcattcaa actcgatggc aacctggccc atgcacgtaa ctgcgaaaac gtcgccaatt
     5461 tcgatagcga caaagcgcaa ctggttccgg tgcgtctgga agaatatgcc ggattcgtct
     5521 tcatcaacat ggaccccaac gccaccagcg tagaagatca attacccggc ctgggcgcga
     5581 aagtgctgga agcctgcccg gaagtccacg atctgaaact ggcggcccgc tttaccaccc
     5641 gcacgcctgc caactggaag aacattgtcg ataactatct cgagtgctat cactgtggtc
     5701 cggcgcatcc aggtttctcc gactccgtac aggttgatcg ttactggcac accatgcacg
     5761 gtaactggac gctgcaatac ggtttcgcca aaccgtccga acagtcgttt aaatttgaag
     5821 agggtacgga tgcggcattc cacggtttct ggctgtggcc gtgcacgatg ctgaacgtca
     5881 ccccgatcaa agggatgatg acggtcattt atgaattccc ggtggattct gaaactaccc
     5941 tgcaaaacta cgatatttac ttcaccaatg aagagttaac cgacgagcaa aaatcgctga
     6001 ttgagtggta tcgcgatgtg ttccgtccgg aagatttacg tctggttgaa agcgtacaga
     6061 aagggctgaa atcgcgtggc tatcgtggtc aggggcgcat catggccgac agtagcggta
     6121 gtggcatttc cgaacatggt atcgcccatt tccataatct gctggcgcag gtgtttaagg
     6181 actaatgaca tcggcggcgg tattttccgc cgctgggctg atttttgatg gagtacagca
     6241 atgtcagact atcaaatgtt tgaagtacag gtgagccagg ttgaacccct taccgaacag
     6301 gtgaaacgct tcacgctggt ggcaaccgat ggcaaaccat tacctgcgtt taccggagga
     6361 agtcacgtca ttgtgcagat gagcgatggt gataaccagt acagcaatgc gtattcacta
     6421 ctgagttcgc cgcatgacac ctcttgttat cagattgccg ttcggctgga ggaaaactcg
     6481 cgcggcggtt cccgcttttt gcatcagcag gtaaaagtgg gcaatcggtt aacgatttca
     6541 acgcctaata acctgtttgc gctaattccc tcagccagaa agcatctgtt tatcgcgggc
     6601 ggtattggta tcaccccttt cctgtcgcac atggcagagc tgcaacacag cgacgtcgac
     6661 tggcagctac attactgctc gcgaaatcca gaaagttgcg catttcgtga tgagctagtc
     6721 cagcatccgc aggctgagaa agttcacttg catcactcat caaccggaac acgactggaa
     6781 ttagcgcgat tattggcgga tatcgaacct ggcacacacg tttatacctg tggtcccgag
     6841 gcgctaaatg aagcggtaag aagtgaagct gcgcgtctgg acatcgccgc cgatacgctg
     6901 cactttgagc aatttgctat cgaagacaaa accggcgatg cctttaccct ggtgcttgcc
     6961 cgttccggaa aagagtttgt ggtgccggaa gagatgacta ttttgcaggt tattgaaaat
     7021 aataaagccg cgaaagtgga atgtttatgt cgtgaagggg tatgcggaac ctgcgaaaca
     7081 gcaatactgg aaggtgaagc tgaccatcgg gatcagtatt ttagcgatga agagcgtgcc
     7141 agccagcaaa gtatgttgat ctgttgttcg cgtgcgaagg gtaaacgcct ggtgttggat
     7201 ttgtagtttg ccaaagccgg atgtggcgct gagcgcgccc agtccggctt cggaagattt
     7261 tactgcggat attcctgcaa taaattgtgt aatgcttccg ccatcagctc accacgccag
     7321 ccggaaatca gctccggcaa attgttctgc ggtttcagtt tccagtgcca gttcagcagt
     7381 tggttgattt gccgacgcga tgccagcaat tcggcgctga tcttatgcgt ttcgctcacg
     7441 tcagtaatca gcgacttaat cgctttaaac gctttacgat aacccggcat gtccatcagg
     7501 ttaagcatcg gctgcggtaa ggcctcttcc ggcaatgtct gcgctttttc caccagcgcc
     7561 agcagcgttt taccgtgaaa gcggatttcg ctaccggata aacccaggct gtccagttcg
     7621 cctaaacttc ccggcatata acgcgcgacc gaccacaaat gctcttcacg cacgacaaag
     7681 ttcaccgcca gatcacgctc acgagccttg cgcagtcgcc agtcggctaa cagttgcaga
     7741 caggccagtt ggcgtgtgcg taattgccag gcattggtca tatcacgcca ggcatcttcc
     7801 ggcgcaacga cttcctgacg acgcatttgc atcaggcggc attcatccag cgccgcaggt
     7861 agccagccgg aggcctccgt ttctaccata agcttggcgg tgatcggtaa cagataccag
     7921 acatcagccg ctgcgtattc acactgacgt tcggtcagcg gtctggccag ccagtcggtg
     7981 cgcgattcac tcttgtccag cgtaacgccg gaatactctt ccaccatgga agcgaacccc
     8041 catgacatcg ggcgtccgca gaaggcagca aggatttgcg tgtcaatcag gggttgtggt
     8101 aattcgccaa agacattgag gaacacttcc agatcttcac tgcctgcatg gagaaatttt
     8161 gtgatggacg gatcgcgcag gatcgctttc agcggtgacc agtcggtgat cccgagagga
     8221 tcaattaacg ccagatgctc accatcaaaa agttgaatta aacccagctg cgggtaataa
     8281 gtgcgcgtac gaacaaattc agtatccagg gctatcgccg gaaaggcacg gacggcttca
     8341 cacaaagaag ccagcgcatc gtccgtggta atcatttggt aattcaaatt gtattctctt
     8401 tagtgggcgt caaaaaaaac gccggattaa ccggcgtctg acgactgact taacgctcag
     8461 gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt ctcgtcgcaa aatttttccg
     8521 acgttagatt tcggtaactc atcacgaaac tccaccagct tcggtacttt gtagcccgtg
     8581 agctgacggc ggcaaaaggt caccagtgac tcttcggtaa gcgatggatc ttttttcact
     8641 acgaagattt tcaccgcttc accactggag ccggaaggta cgccaacagc cgcgacttcc
     8701 tgtacgccag gatgctgcat gacgacatct tcaatctcgt tgggatagac gttaaaaccg
     8761 gaaaccagaa tcatgtcttt tttacgatcg acaatgcgca ggaacccttc ttcatccatc
     8821 accgcgatgt cgccggtgtg taaccagcca tttttgatga tttcatcggt agcatcggga
     8881 cgctgccagt aacccagcat cacctgcggt cctttgacac aaagctcacc cggttgacct
     8941 ggtggtactt cattatcatc atcatccacc agtttggctt ccgtcgacgg caccggcaaa
     9001 ccgatgctac cactatgata atcaatatca tatgggttaa cgctgaccag cggcgcacac
     9061 tcggtaaggc catagccttc cagcagatac tgtccggtca gtttcaccca acgctctgcc
     9121 accacttgct gcactggcat cccaccgcct gcggaaagat gcagactgga gaaatccagc
     9181 tgctggaact ctttattgtt cagcaacgca ttgaacaagg tgttaacgcc agtgatagcg
     9241 gtaaacggat atttcgctaa ctcttttacc aaccctggaa tatcacgcgg gttagtgata
     9301 agcaggttct gcccacccag ttcgataaac agcaggcagt taatggtcag ggcaaaaatg
     9361 tgatacagcg gcagtgccgt tactaccagt tctttacctg gatgcagcaa cggcccatag
     9421 gtggcgttga cctgttccag gttcgccagc atattgcggt gagtcagcat cgcgcctttc
     9481 gccacaccgg tggtgccgcc ggtgtattgc aggaaggcta aatcttccgg caccagttcg
     9541 ggtttgacgt actgcatccg gtagccgtta tgcagtgcgc tacgaaatga aatggcatct
     9601 ggcagatggt atttcggcac caaacgcttg atgtatttaa caacgaaatt gactaccgtg
     9661 ccttttgccg tagatagctg atcgcccata cgggtcagaa ttacgtgctg aacggcggtt
     9721 ttatcaacca ctttttccag tgtgtgagca aagttagaca cgataacaat cgccgatgcg
     9781 ccgctatcgt taagctgatg ctcaagctca cgcggggtat acaacgggtt aacgtttacg
     9841 acgatcatcc cggcacgcaa aatgccaaac agcgccaccg gatattgcaa taaattaggc
     9901 atcatcaacg caacgcgatc gcctttcttc agccccaacc cttgttgcaa ataagcggca
     9961 aacgcgcgac tgcgttcttc cagcttgcgg aaggtcatca cctcccccat attcacaaac
    10021 gcaggctgat cggcgtagcg cgcgaccgac tgctcaaaca tatctaccag agattgataa
    10081 cggtcaggat tgatctccgt cggaacgtcc gcgggataac ggttaagcca aaccttcttc
    10141 aattcttcac ctctaaaatg cgtgttcgtc gtcatcgcaa ccccaaatga tatacatgct
    10201 gttaacataa tattaactca tcataccagc ttgataatta cccaacgaaa aggttgcgaa
    10261 gcgcgtcact atttattttt atctttaccg taagaatgca gaaacagcgg accagccgct
    10321 gtttcttttt ctttaaaaac aagcaattat tcagttacaa ctgtttgtac tctcgcggga
    10381 ccgggattat accagcccca tccgccgtag ccatagggcc agccacgacc gccataaaac
    10441 catggatcaa tcggctgagg cggcataatc acctgctggg ttaaatgcca acgtttgtaa
    10501 cccgttactt gcatcaccat aaatttatag ggcgtattgc cgattttgcc gtcaaccgca
    10561 ccagtgattg gcccgactac cgtaaccagt tgtccacgga aatccaccgg gtccagaaaa
    10621 ccgttcacat cggcataaat gcgaccgcga gaaggttctc ccagcgtcgg tctggctccg
    10681 ctgtccagcg gtacggtagc aatttccagg cgggttttcc cttgctggtt ttgtaccgca
    10741 accactttgc caccaaagcg tgcctcctga ccaacgtaca gctgcggcgc actcatcacc
    10801 cgaactaaat cttgttgcgg cgtgggactg ctgcctttaa tggcgtccgg cacagtgaca
    10861 caaccgctca gcattagcgc aaacgtacct gccagtatgc ctttgataac attcttttga
    10921 accgccatgg tgcgactcct ttttttctca gggcatactc ttaagattca ttctttgccc
    10981 ggaagtttct tccatgcgac gttgttacgt aaataaaccg gttcggcatg ttccaccgcc
    11041 accgttttac cctcggcaaa catctgacac gcaatcggca gcatatcttc agcagcaggc
    11101 agtaacactt cgccatcgcg caaaaccagc ccgctctctt taccgagatc cggccaagct
    11161 tgccagcccg tccctaccgt cacccattcg ccggaaagct gttgcattcg ttcatggacg
    11221 atttcgggtt tgagtacggc ttcggtttct tcaccgtgcc agataccgtt ttcatcacgc
    11281 tgatattcgg cccagtaaac ttcgcccatt cgcgcgtcaa tggctgccag cacgcgggtt
    11341 gcgccgtttt tgcgccacgc cccttgcgcc atcgtcatta gcgtggagac gccaatcatc
    11401 ggtaattccg cgccaagcgc cagcccttgc gcgatgccaa taccaatgcg cacgccagta
    11461 aagctaccgg ggccgcgccc gtaagccaga gcgttaatat cagtcaggga agttccgctg
    11521 gtggtcagga tatcctgcac catcggtaag attcgttgag tatgttcacg agggcaaagc
    11581 tcaaaatgag cgttgacagt accgtcgttc cacagggcga cagagcaggc ctctgtcgcg
    11641 gtatcgatag ccagaattcg catgggtctt cgtgcttaga tcaataaaaa ggcgcgcatc
    11701 ataccatact ccgtaacaaa ttacctggag gatggtatcg caaggaaacg aaccgcacgg
    11761 gcaatgtcac gggtgcgtgg cgcgggcggc agactggcga gaaacgtcgc gccgtaagga
    11821 cgcatcacca gccgattgtc acaaatcacc agcacgccac gatcgtcggc gtcgcgaatc
    11881 agtcgcccta ccccctgttt gagagtaatg acggcatctg gtagttgcac ttcatcgaac
    11941 gggtcgccac cgcgcaaacg acaatcttcc atgcgcgctt ttaacagtgg atcatccggc
    12001 gaggtaaacg gcaatttgtc gataattacc aatgacaatg tatcgccacg cacgtccacc
    12061 ccttcccaga aactactggt tgccacaaga agcgcattac cggcactgac aaactgctgc
    12121 aacagttggc ctttactggt ttccccctgc aacaaaacgg gaagcgtcat ggtagcgcgg
    12181 aactgctcgg ccagatcgcg catcatggcg tgcgaggtac aaagcataaa acaacgaccg
    12241 ttgttagctt cgatgatcgg tcgcagcatt gccgctaact ggcgagcaga acctggctgg
    12301 ttggtttgcg gcagattgcg cggcacacag agtaacgcct ggcggctgta atcaaacggg
    12361 cttggcaata gcaacgactc cgcctgttct atcccaagcc gcgaggtgaa atgatgcaga
    12421 tcgtcgttca ccgacagcgt tgctgaggtg aagatccagc taccgggctt ttgcgccatt
    12481 aactctttga atttatccgc cacgctgagc ggcgtgagag ccagagtaaa atggcgcgaa
    12541 gtgcattcgt accagtagct gtagcccggc tgattgatct cttttagccg cttcagccgt
    12601 gtgcgataca acgtggcgcg ctcaaatgcc gcatccagca aggcggaacg ccccagtgac
    12661 agttttgcca cgtcataaca aagttccagg gtgtcatcga gcagtaaaaa tgcccgctga
    12721 atttgcggat tagctaacag ctcacgcagg ttaccgcgat aacctggctc accgagttgc
    12781 agacgaaaat cctgcgcact ctgggcaaga cgatcagcgc acttttgtaa ctgctgggtg
    12841 tcttttaatt cggtgcggta ggcgatggtg atgtcttttg ccaggtcgag cagttgtcga
    12901 ctggagagtg actgaccaaa atactggctg gcaatgtccg gtagctggtg ggcttcgtcg
    12961 aagatcatga cgtccgcttc cgggatcagt tcgccaaatc cactctcttt aaccaccata
    13021 tccgccagaa agagatgatg gtttaccacc accacatcgg cgtccatcgc ttttttacgt
    13081 gctttgacca caaagcaatc tttatacatc gggcagtcgc tgccaagaca gttgtcgttg
    13141 gtgctggtga ccagcggcca cgcctgtgaa tcttccgcca cgctgacgca ggtgctgata
    13201 tcaccatcga ctgtttgatt agaccaggag cgcagcagga tcacatcgct taagatttgt
    13261 accggcagat cgccccccgc cagcgcctgc tgttcgagac gttcgaggca gaggtagttt
    13321 gagcgccctt tcagcagcgc cacgttgccc gtatatttca atgcctttga gactgttagc
    13381 aaatcgcggc tgtagagctg atcctgcaac gcttttgagc cggtcgagat aatgactttc
    13441 tttttcgccc gcagcgcagg agccaggtaa gcgtaggttt tgcccgtacc ggttcctgct
    13501 tccaccacca gcggctggcc tttttctatc gcctgggtga cggctaccgc catctgtcgc
    13561 tgtggttctc gcggcttaaa gcctggtatc gctttcgcca gctgaccgtc tggtgcaaaa
    13621 tcgtccgtca cactaccccc tgttgatttg aacagggatt atgtcaggat gagggcgctt
    13681 tcgccagttg aagtggtgac ggcgacctca cattgtggca gtctttgcag cacgaaaatg
    13741 gaagtataat gaggacaaaa tgatgactat cgttcgtatc gatgctgaag cccgctggtc
    13801 tgatgtagta atccacaaca acacgctcta ctacactggt gtaccggaaa acctcgacgc
    13861 cgatgccttt gagcaaaccg ccaacacgct ggcacagatt gacgccgtgc tggaaaaaca
    13921 gggcagcaat aaatcgagca ttctggatgc caccattttc ctggccgata aaaacgactt
    13981 cgcggcgatg aataaagcgt gggatgcctg ggttgtggcg ggtcatgcgc cggtgcgctg
    14041 cacggtgcaa gcggggctga tgaacccgaa gtataaagtt gaaattaaga ttgtggctgc
    14101 ggtgtaagct ttatcgaagc aaaataagtc agacgataat ttatcgataa tactggtcgg
    14161 ttttacataa atcgaccaga gattaagata ttactgttga tgctcctgct aattcattat
    14221 ttatttttat aatttcaatt ttagctataa agctaactat ataaataaac ataaccatgc
    14281 cgttgtgttc gcccttcgat tagcacctct ggttttcttt cttagctgaa caatctggtt
    14341 atagcgtgcg gactgaccgt taattttctc catcttactg gataacacac catgagctgc
    14401 tacctttaaa acacctgaat gtcaacaggt taactcgtgc catatcgtta taatcccttt
    14461 gatattagat gcaaattaag gtcatatata gccttatcga taacatggtt aattttaagg
    14521 ataagaatat gcctgctgta atagataaag ccctggattt cattggtgcc atggatgtat
    14581 cagcgccaac accaagttcg atgaatgaaa gcacagcgaa gggaatcttt aaatatttaa
    14641 aagaactggg agtacccgcg agtgccgctg atattacggc gcgagctgac caggaaggct
    14701 ggaaccctgg gttcacggaa aaaatggttg gatgggcaaa aaaaatggag tctggtgaac
    14761 gcactgtaat taaaaatcct gaatactttt caacatatat gcaggaagaa ctaaaagcac
    14821 tggtctgagt ttaatttata tcagcataaa tgggtcagga cgcttttaat cacatataaa
    14881 aagcgtcctt tttctcacaa tcaaataatc atcggattct ataacgaaat cgatacggat
//
LOCUS       DAJOSY010000102        14665 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJOSY010000102 DAJOSY010000000
VERSION     DAJOSY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.102"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            93..1202
                     /gene="anmK"
                     /locus_tag="OHN27_003433"
     CDS             93..1202
                     /gene="anmK"
                     /locus_tag="OHN27_003433"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCQ2287813.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            1300..1629
                     /gene="mliC"
                     /locus_tag="OHN27_003434"
     CDS             1300..1629
                     /gene="mliC"
                     /locus_tag="OHN27_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCQ2287814.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            1688..2344
                     /gene="pdxH"
                     /locus_tag="OHN27_003435"
     CDS             1688..2344
                     /gene="pdxH"
                     /locus_tag="OHN27_003435"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCQ2287815.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            2473..3747
                     /gene="tyrS"
                     /locus_tag="OHN27_003436"
     CDS             2473..3747
                     /gene="tyrS"
                     /locus_tag="OHN27_003436"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCQ2287816.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            3806..4669
                     /gene="pdxY"
                     /locus_tag="OHN27_003437"
     CDS             3806..4669
                     /gene="pdxY"
                     /locus_tag="OHN27_003437"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCQ2287817.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(4713..5318)
                     /gene="gstA"
                     /locus_tag="OHN27_003438"
     CDS             complement(4713..5318)
                     /gene="gstA"
                     /locus_tag="OHN27_003438"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCQ2287818.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(5424..6926)
                     /gene="dtpA"
                     /locus_tag="OHN27_003439"
     CDS             complement(5424..6926)
                     /gene="dtpA"
                     /locus_tag="OHN27_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCQ2287819.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(7537..8172)
                     /gene="nth"
                     /locus_tag="OHN27_003440"
     CDS             complement(7537..8172)
                     /gene="nth"
                     /locus_tag="OHN27_003440"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCQ2287820.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(8172..8867)
                     /gene="rsxE"
                     /locus_tag="OHN27_003441"
     CDS             complement(8172..8867)
                     /gene="rsxE"
                     /locus_tag="OHN27_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCQ2287821.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(8871..9491)
                     /gene="rsxG"
                     /locus_tag="OHN27_003442"
     CDS             complement(8871..9491)
                     /gene="rsxG"
                     /locus_tag="OHN27_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCQ2287822.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(9495..10553)
                     /gene="rsxD"
                     /locus_tag="OHN27_003443"
     CDS             complement(9495..10553)
                     /gene="rsxD"
                     /locus_tag="OHN27_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCQ2287823.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCAMLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(10554..12680)
                     /gene="rsxC"
                     /locus_tag="OHN27_003444"
     CDS             complement(10554..12680)
                     /gene="rsxC"
                     /locus_tag="OHN27_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCQ2287824.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDP
                     RKAAVAAAIARAQAKKAAQQKVVNED"
     gene            complement(12673..13251)
                     /gene="rsxB"
                     /locus_tag="OHN27_003445"
     CDS             complement(12673..13251)
                     /gene="rsxB"
                     /locus_tag="OHN27_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCQ2287825.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(13251..13832)
                     /gene="rsxA"
                     /locus_tag="OHN27_003446"
     CDS             complement(13251..13832)
                     /gene="rsxA"
                     /locus_tag="OHN27_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCQ2287826.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(13909..14349)
                     /locus_tag="OHN27_003447"
     CDS             complement(13909..14349)
                     /locus_tag="OHN27_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCQ2287827.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(14435..14650)
                     /gene="ydgT"
                     /locus_tag="OHN27_003448"
     CDS             complement(14435..14650)
                     /gene="ydgT"
                     /locus_tag="OHN27_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCQ2287828.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT         3675 a         3743 c         3780 g         3467 t
ORIGIN      
        1 taccaacggt aaaaatcggt cattccgcat ggtattcacg aaaagtttat gtttgagtgg
       61 tgcgcaaacg tcagattgaa aaaggatgaa ctatgaaatc gggccgcttt attggcgtta
      121 tgtcaggcac cagccttgat ggtgttgatg ttgtgttggc gacaattgat gaacaccggg
      181 tcgcacagct ggcaagtttg agttggccga tcccggtatc tctgaaacag gctgtactgg
      241 atatttgcca gggccagcag cttacacttt cgcagtttgg acagcttgat actcaactcg
      301 ggcgactttt tgctgatgcg gtcaatgcct tgcttaagga acaaaatctg caggcaagag
      361 atatagttgc gatcggttgt cacggtcaaa ccgtctggca tgaaccgacg ggcgtggcac
      421 cacacacttt acagattggc gataacaatc aaattgtggc acgcaccgga attacggttg
      481 tcggtgattt tcgccgtcgc gatattgcct tgggaggaca aggcgcaccg ctggtacctg
      541 cgttccatca tgcgctgctg gctcacccaa ccgagcgacg aatggtgctc aatattggcg
      601 gcatcgccaa tctgtcactg ctcattcctg ggcagccggt tgggggctac gataccggtc
      661 ctggtaacat gctgatggat gcctggatct ggcgtcaggc cggtaaacct tacgataaag
      721 atgccgagtg ggcacgggcg ggtaaagtta ttctcccact gctgcaaaat atgctcagcg
      781 acccgtattt ctcgcaacct gcaccgaaaa gcaccggacg cgaatacttt aactatggtt
      841 ggctggagcg ccatttgcgc cattttccgg gtgttgatcc ccgagatgtg caggcgacac
      901 tggcagaact caccgccgtg accatttctg aacaagtttt gttgagcggt ggctgcgaac
      961 gattgatggt atgtggtgga ggtagtcgta atccgctact catggcgcgt ctggcggcat
     1021 tactgccagg cacagaagtc accaccaccg atgccgttgg cattagtggc gatgacatgg
     1081 aagcattggc tttcgcctgg cttgcctggc ggacgctggc gggattacca ggaaatctgc
     1141 cttccgtcac tggcgcaagc caggagacgg tactgggggc tattttcccc gctaacccgt
     1201 gatatcagag ttaactgaat ttttcttacc gcgtctgccg ttacacttgg aacgttaggg
     1261 agggcgtatt gccctccaga ccaggaaagt cttcgggata tgactatgaa aaaactgtta
     1321 attatcattt tgccggtcct tctctccggc tgtagcgcct ttaatcagct ggttgagcgc
     1381 atgcaaaccg atacgctgga ataccagtgt gatgaaaaac cgttgacggt caaactgaat
     1441 aatccgcgcc aggaggtcag ttttgtttac gataatcaac tactgcatct caaacagggc
     1501 atttcagcct ctggcgcgcg ttacactgac ggaatctatg ttttctggtc gaaaggcgat
     1561 gaagcgactg tctataaacg cgaccgcatc gtcttgaata actgtcagtt acaaaatcca
     1621 cagcgttgag atttttccag gggcggcgca caatagcgtc acccactgac aatccgtaaa
     1681 gaaaaccatg tctgataacg acgaattgca gcaaatcgcg catctgcgcc gtgaatacac
     1741 caaaggcggg ttacgccgcc gcgatcttcc cgccgatcca ttaacccttt ttgaacgttg
     1801 gctctctcag gcttgtgaag ccaaactggc ggaccctacc gcgatggtgg tcgctaccgt
     1861 ggatgaacat ggtcagcctt atcagcgcat cgttttactc aaacattacg acgaaaaagg
     1921 catggtgttt tacaccaacc tcggcagccg taaagcacat caaatcgaaa ataatccgcg
     1981 cgttagcctg ctgttcccgt ggcataccct tgagcgccag gtgatggtga tcggtaaagc
     2041 agaacgactt tcgactctcg aagtgatgaa atattttcat agccgcccgc gtgatagcca
     2101 gattggtgca tgggtttcga agcagtccag tcgcatttct gcccgcggta tccttgaaag
     2161 taaattcctg gagctgaagc agaagtttca acagggcgaa gtgccattgc cgagcttttg
     2221 gggcggtttt cgcgtcagcc ttgaacagat tgagttctgg cagggtggtg agcatcgcct
     2281 gcatgaccgc tttttgtacc agcgtgaaaa tgatgcgtgg aagattgatc gtcttgcacc
     2341 ctgaaaagat gcaaaaatct tgctttaatc gctggtactc ctgattctgg cactttattc
     2401 tatgtctctt tcgcatctgg cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac
     2461 atggagattt tgatggcaag cagtaacttg attaaacaat tgcaagagcg ggggctggta
     2521 gcccaggtga cggacgagga agcgttagca gagcgactgg cgcaaggccc gatcgcgctc
     2581 tattgcggct tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt tccattgtta
     2641 tgcctgaaac gcttccagca ggcgggccac aagccggttg cgctggtagg cggcgcgacg
     2701 ggtctgattg gcgacccgag cttcaaagct gccgagcgta agctgaacac cgaagaaact
     2761 gttcaggagt gggtggacaa aatccgtaag caggttgccc cgttcctcga tttcgactgt
     2821 ggagaaaact ctgctatcgc ggcgaacaac tatgactggt tcggcaatat gaatgtgctg
     2881 accttcctgc gcgatattgg caaacacttc tccgttaacc agatgatcaa caaagaagcg
     2941 gttaagcagc gtctcaaccg tgaagatcag gggatttcgt tcactgagtt ttcctacaac
     3001 ctgttgcagg gttatgactt cgcctgtctg aacaaacagt acggtgtggt gctgcaaatt
     3061 ggtggttctg accagtgggg taacatcact tctggtatcg acctgacccg tcgtctgcat
     3121 cagaatcagg tgtttggcct gaccgttccg ctgatcacta aagcagatgg caccaaattt
     3181 ggtaaaactg aaggcggcgc agtctggttg gatccgaaga aaaccagccc gtacaaattc
     3241 taccagttct ggatcaacac tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc
     3301 tttatgagca ttgaagagat caacgccctg gaagaagaag ataaaaacag cggtaaagca
     3361 ccgcgcgccc agtatgtact ggcggagcag gtgactcgtc tggttcacgg tgaagaaggt
     3421 ttacaggcgg caaaacgtat taccgaatgc ctgttcagcg gttctttgag tgcgctgagt
     3481 gaagcggact tcgaacagct ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc
     3541 gcagacctga tgcaggcact ggtcgattct gaactgcaac cttcccgtgg tcaggcacgt
     3601 aaaactatcg cctccaatgc catcaccatt aacggtgaaa aacagtccga tcctgaatac
     3661 ttctttaaag aagaagatcg tctgtttggt cgttttacct tactgcgtcg cggtaaaaag
     3721 aattactgtc tgatttgctg gaaataatgc attaagtgga aagggggagt gagaaatcac
     3781 tccccctggt ttttatacag ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt
     3841 tgtttatggt catgcgggta acagtgcggc agagtttccg atgcgccgcc tgggcgcgaa
     3901 cgtctggccg ctgaacaccg ttcaattttc taatcacacc caatacggca aatggactgg
     3961 ctgcgtgatg ccgcccagcc atttaaccga aattgtgcaa ggcattgccg ccattgataa
     4021 attacacacc tgtgatgccg tattaagtgg ctatctggga tcggcggagc agggtgaaca
     4081 tatcctcggt atcgtccgtc aggtgaaagc cgcgaatccg caggcgaaat atttttgcga
     4141 tccggtaatg ggtcatccgg aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca
     4201 tgtgcggcac ggtttgcctg ccagcgatat cattgcgcca aatctggttg agctggaaat
     4261 actctgtgag catgcggtaa ataacgtcga agaagcggtt ctggcagcgc gcgaactcat
     4321 tgcgcaaggg ccacaaattg tgttggttaa acacctggcg cgagctggct acagccgtga
     4381 ccgttttgaa atgctgctgg tcaccgccga tgaagcctgg catatcagcc gtccgctggt
     4441 ggattttggt atgcgccagc cggtaggtgt tggtgatgtg acgagcggtt tactgctggt
     4501 gaaactgctt cagggggcaa cgctgcagga ggcgctggaa catgtgaccg ctgcagtcta
     4561 cgaaatcatg gtgaccacca aagcaatgca ggaatatgag ctgcaagtgg tggctgctca
     4621 ggatcgtatt gccaaaccag aacattactt cagcgcaaca aagctctgaa atatctcatt
     4681 tcggcccaca acgacctgtg ggccgatggc tattacttta agccttccgc tgacagcgcg
     4741 tcttgtactt ccggacgttc agccatacgt tgcataaatg ctgcaatgtg ctctaaccct
     4801 tccagattca gtttcaccgc gtatgcccag cgcagaaccg taaacagata ggcatcagca
     4861 attgtaaatc tttgcccgca gatccagtgc tcatccttca gtgcctcgtt cacatattgc
     4921 agcttcttct ccagctgcgc gcgaactgtc ggtttgtact cttccggtgt atcagggcga
     4981 aacagaggtg tgaaaccttt atgcagctcg gtggcgatgt aattcagcca ttcgatggtt
     5041 ttatagcggg aaatactgtt taccggtgcc agcaactggc ggtcggggac gctgtcggca
     5101 agatactgca taatcgctac gccttccgtc agcaaagtac cgtcatccag cagcaatgca
     5161 ggcacctgcc ccttagggtt aacggcaaag taatcgtcac cgttttcgag acgttttttc
     5221 attaaatcca cactgacgag ggtaaaatcc tttccgctct cacgcagggt gatatgggaa
     5281 gcgagagagc aggcacccgg tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg
     5341 tttaaagtat ggtagtgcgt tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc
     5401 ttgtaaaaga gtttccctga attttacgct acggctgctt tcgccgcttt gtctgcagcg
     5461 tcatcctgcg tcatgcggtg cagtttcggc gcggtcagca gcatcagtac tgcaataacg
     5521 gcagtagcga caccaatctg caagaatacg cgaccataga cttccagtga catcagcgga
     5581 tcggtaacgt tatccggcac agccatcata cccgcaacat aaccaccaat caggtttgca
     5641 ccggcagtgg tcaggaacca gctacccata atgaagccca tcagacgctg cggaacgagt
     5701 tgagcaacca ttgccagacc cagaccagag atcatcagtt ccccgatgct ctgcaggcca
     5761 tagcttgcga ccagccagct tacagacacg ataccagcgt cagacgcgaa tttcgcaccc
     5821 agcggcagaa tcaggaacgc accagaacac atcaccatgc cgattgcaaa cttggttggc
     5881 atcggcaggg tatcgcccat cttgttatag atagcggcca gaatcggact accgatgatg
     5941 atccagaacg ggttcagtgc ctgatactgc tcaggttcta cggccagacc cagaatggag
     6001 tgctcaacgt tacgaatcgc aaagaagttc agtgacgttg gcatctggct gtacagcacg
     6061 aagaagataa tggcttcgag catcaggatg aaggcaacga tcattttacg acgcgcagca
     6121 cctttcatcg cgaaggcttc tttaccgaag ataaccacga taccgaaggc aacaacgccc
     6181 agcgccatac gcgcaacttc ctgattgtgc agcagccagg tggcgatagc gatcagtgcc
     6241 acaacaccaa taatggtcag cagcaggtta cggtagttga taggctcgaa gtctggtttt
     6301 gaaccgtact gtttaaccca gcgttggcag aaggcgaagt taacgatagt gatcagcagg
     6361 cctacaacgc tcaacgcaaa cgcaacactc cagccgtatt tcgcggccag ccacggcgta
     6421 gcaatcatag agaagaaaga gccgatgttg acggacatgt agtacatggt gaatgcaccg
     6481 tccagacgcg ggtcgttttt ctcatagcat gtagaaagca gagaagacgg gttagcttta
     6541 aacaggccgt taccgaccgc aatagccgcc atacccatat aaacgatacc ggcgtcgtga
     6601 ccagaccagg caaccagagc ataaccaatc gccagcacaa tagcgccgag cataattacg
     6661 cgtttagtac ccagtacctt gtcacctaac cagccgccga tagcgaccag accataaacc
     6721 agggcactaa aggaagagaa aagggtgatt gaatccgctt cagacatacc cagttgttta
     6781 accaggtaaa cagccataat tccttgtagg ccgtaataac caaaacgttc ccataactca
     6841 atcgagaaga tgagatagaa cgccttcggt tgtttgaaag cgttcaaact gacgctttca
     6901 gttggttttt ggtttgcagt ggacactttt acctcttttt ttacatccca tattaacggg
     6961 ggtgctttct cgcgttatgt ataaaaaaca tacgcgcaat tgttatagtg ggaggggaaa
     7021 cggcaggtaa tgttcactat cttgcacttt cagacaagtc ctttgtaata atctgttaca
     7081 tataactgag gcggggaatt caaccattcc gggaaatgtt atccagcgtt aaatttttca
     7141 aaaaacgtaa cggcagattt tttcactgca ctgagagggg ggtgatggct taacggcgat
     7201 atgttctgct atttagcgtt tttactagtg agatagtcca gtttctgaaa aatagccagt
     7261 gtaatgtttt gtaggtcaat atttgttgcg tgtgtaaatt taaatgattt taacctgatt
     7321 cttgcaatgt ttttacattg cgacagcatc gtgatcttga tcacgaatga atagaaaatt
     7381 tcgctattaa aaaaacaatt aacctgcatt tttggttatc cgcaaacgga ttacctgatg
     7441 ataacgctaa gcaatggcat cattgacgtt aaaaagatgt tttaaaagag gataaagaaa
     7501 ggttatcaat ggggtaatcg gtgttacccc ttttcttcag atgtcaactt tctctttgta
     7561 ttcacaaaga tcttcaataa tacaagagcc acagcggggc ttgcgggcaa tgcaggtata
     7621 acgcccgtgc aggatcaacc aatggtggca gtcgacttta aactctgccg gaaccacttt
     7681 cagtagcttt tcttctacct gttcgacgtt tttccccggc gcaaattgag tacgattaca
     7741 aacgcggaaa atgtgcgtgt cgacagcaat agtcggccag ccgaatgcag tgtttaatac
     7801 gacgttggct gttttacgac ctacgccggg cagggcttca agcgcagcac gatcttccgg
     7861 aacctcgcca ttatgctgct ccagcaagat acggcaggtt ttgatgatat tttctgcttt
     7921 gctgttataa agcccaatcg ttttgatata ggttttcacc ccttcaacgc ccagttcaag
     7981 catcgctgca ggcgtattcg ccaccgggta gagtttcgcc gtcgccttat taacactgac
     8041 atcggtcgcc tgagcggaaa gcagtacggc aatcagcaat tcaaaaggcg aactgaaatt
     8101 aagctcggtg gtgggatgag gattgttctc acgcaggcga gtgaggatct ccaggcgttt
     8161 tgctttattc atcagacatt ccctgtttca ccgtttggca atgcacgttc tgcagctgct
     8221 tcagcacggc gctttttcat tctttcatca atcaggtatt ttcctgccag catcagtccc
     8281 aggccaataa atgcacctgg tggcagcatc gccagcagga aaggggagtc ggtgtggaaa
     8341 atctccacgc gtaatacttt tgcccagcta cctaacagcg catctgcacc gtcaaacaat
     8401 gtgccattgc cgataatttc gcgtagtgaa cccagcacga acatggcgca ggttgcgccc
     8461 ataccaattg aaaagccgtc cagtgccgaa agcgccggac cttttttggc ggcgaaggct
     8521 tcagcgcggc ccacaacgat acagttagtg acaatcagcg gaataaaaat ccctaatgat
     8581 tgatacaggc caaaggcgta ggcgttgatc agcatctgta cagcgctgac caccgaggcg
     8641 atgatcatca cgtaaatggg aatgcggatc tcggctggcg tccagtgacg cagcgtcgaa
     8701 atggtcaggt tggtcagcgt cagtaccagc gtagtcgcaa gtcctaaacc cagagcgtta
     8761 gtggcagtgg acgtgaccgc caacagagga caaaggccga gcaactggac cagcgcagag
     8821 ttgtttttcc acaacccctg aacaataacg tctttaattt cgctcacgct ttattctcca
     8881 caggcaggaa gttgagaaag ttgtgccggt aacgtctgag cgtacaatcc ggcgcgtttt
     8941 accgcattaa ccaccgcgcg gggagtaatc gtcgcgccgg tgaactggtc gaaatcacca
     9001 ccatctttct tcaccgccca gtgcgcatca tctgcaccac tgattttttt acccgcaaaa
     9061 tgggtgatcc agtcagaaag gcgcagttcg attttatcgc caagccctgg cgtttcgtgg
     9121 tgctctgtca cgcgcgtgcc aagtaccgtg ccgttaaaat cggctcccac cagcagctga
     9181 atcgcaccgg aatagccatc tggcgcggtt gcttccagaa cggcggctac cggtttgtca
     9241 tcctgtttgg cgatgtaaac ccgatgctca cctttaccta actctggcgc agttaccaga
     9301 tagcaactct gtgccagcgc attgttatag cgttcggctg gcagcacctg atcaaataac
     9361 gccttttgtt gcagactggc ctgttcagca atcgtcgttt tggtcatctg gttgatggcc
     9421 gcagttaacc ctgttgaacc cgctgcaaat agcgccaacg taatgccgtg ttttcggata
     9481 gttttcagca tggtttaccc tttgcgatgg ccgtagacgc gcggacgcgt gtagtaatcg
     9541 atcagaggaa ccgtgatgtt cgccagcagg acggcaaaag ccacgccgtc aggatagccg
     9601 ccgaaactgc ggatcaacca gactaataag cccgcaagcg cgccgaaaat aagacgacca
     9661 cgattggtcg tagaagcggt aaccgggtca gtcaaaataa agaatgcgcc gagcatggtc
     9721 gctccagaca gcagatgaat ttgcggtgct gccagtgttt ctggtgagaa caaccagccc
     9781 aacattgcgc ataacgccag cgttactaag aagctgaggg gaatatgcca gcgaatcgct
     9841 ttctgccata gcaaccatac gccgccagcc agccaggcga gatttaccca ttgccaacca
     9901 gcgcccgcca gaataccgct gtagatcgga tattgcataa tctgttcaac cgaatgaccg
     9961 gcacggacag aggttttaaa tgtatccagc ggtgtcgcct gactaatgcc atcaatacct
    10021 aagcgtagtg tgttcatatc accaccactg gcggtatgac cgctaaaaat aacctggatg
    10081 gcgtcgataa aaccagggat gttgaccgca atttcatgtg gcggtaacca gctggtcatc
    10141 tgcacgggga aggagatcag taagaccaca taaccaatca ttgccggatt aaacgggttt
    10201 tgtcccagac cgccatacaa ctgtttagcg atgatcaccg caaacaccgt acccagcacg
    10261 accatccacc atggcgcgag ggggggaata cttaccgcca gcaataagcc tgtcagcaat
    10321 gctgagttat ctttcaacgt tgcggctacc gactgcttgc gtagtttgag tacgagagct
    10381 tcggctaaca gagcactaac cgatgccaac aggatctgaa cgagagtacc ccaaccaaaa
    10441 aaccacagtt gcgctgcgat tcctggcacg gctgcgagca acaccaacag cataatgcgc
    10501 gatgtctggc gctggttatg ggtataaggg gagctagcta ttctgaatac catttagtcc
    10561 tcgtttacaa ccttctgctg ggcggctttt ttggcctgag cgcgggcaat agccgcggca
    10621 actgccgctt tgcgcggatc aacctgttct tctggttctg cgttagtctg ttgctgttcc
    10681 agcttgcgcg ctttggcacg ggcaatagcg gcttcgacgg cggctttgcg cggatcaacc
    10741 tgttcttctg gttccgcatt agcctgttgc tgttccagtt tgcgcgcttt ggctcgggca
    10801 atagcggctt cgacggcggc tttgcgcgga tcaacctgtt cttctggttc cgcattagcc
    10861 tgttgctgtt ccagcttgcg cgctttggca cgggcaatag cggcttcgac ggcggctttg
    10921 cgcggatcga cctgttgttc tggttccgca ttagcctgtt gctgttccag cttgcgcgct
    10981 ttggcgcggg cgatagctgc ttcaacggca gttttacgtg gatcagcaac ggttgctgcg
    11041 tcgttagttt gctgcagttc tgcctgtttc gctctggctt gcgctttacg ggcttcccgt
    11101 gctgcaataa ttgcactgtt atccgggcgt tcgcccgctt taatcacaat aggctgtgta
    11161 gcctgggcct gtttctcttt cacccgcgcc agagcggcag caatcgcatc tttatctttg
    11221 gctgcaggtt gaacggctgc gctcttatgt cgttcaaggc gagccgcttt ttcgcgctcc
    11281 agacgagcct ggcgcgcttc gaaacgcgct ttggcttctg cggcgcgctt ttcttcctga
    11341 cgaatagccg caatttcagc tttttcctga cggaaatatt gcaccagggg aatattgctc
    11401 gggcaaaccc acgcgcaagc cccacattca atgcaatcag caatgttatg cgtggtagct
    11461 ttatcgtgtt gctgaccttt gctgaaccag tacaactgtt gcggcaaaag atccgcaggg
    11521 caggcgtcag cacaggcgct acaccggatg cagctttgtt cttcctgtgg ttcgccaagt
    11581 tcattggcag agggagccaa cagacagttg gtaatcttta cgaccgggac atccagccat
    11641 ggcaaggtaa agcccattag cgggccaccc ataatcacca tttgatcggc agaggggcag
    11701 aatccggcat cattcaataa atgacgcact ggcgtcccca gccgtgccca gacgttgccc
    11761 gggcgagcga ttgcttcgcc agtcagggtt acaacacgct cggtaatcgg ctcgccatca
    11821 ataacggcac gtttcactgc ataagcagtg ccgacgtttt gcattaatac gccgatatcg
    11881 gatgaacgcc cgccatgtgg aacctgcttc ccggtcagaa tgtaggttaa ttgtttagca
    11941 ccgccagaag gatatttggt tggaatcacc cgcagagaaa tatcgttaga gtccgccagc
    12001 accgcgcgca gcatggaaat cgcctgcggt ttgttatctt caatgccgat aagaatttcg
    12061 cgtggctgca gaatatgcgc aagaatgcga ataccctcta cgacctgagc cgcgcaatcc
    12121 tgcatcaaac ggtcatcggc ggtaatgtac ggctcgcact cagccgcgtt gataatcaac
    12181 gtttcaatct tatctccgcc accctgcaat ttaacgcctg tcgggaatcc tgcaccgccc
    12241 agcccggcaa caccaaactg atgtatgcgc tcgattaact cttcgcgact gcgagtgcga
    12301 taatcggccc agccgtcgcg cgggatccag cagtcttcac catcggcatc aataatcacg
    12361 cttaattcag ctaaagctga aggatgagcc gtagagtggg gcgcaatagc cgtaacggta
    12421 cccgaggtgg gcgcgtgaac aggcagcatt ttgccgcgac cacgggtaag cggctggccg
    12481 cgcaatactt tatcgccgac gctaacgcac aactcacctt cagcgccaat atgctgtttc
    12541 agtggaataa caaaacgctg cgccaggggt acctggcgca ggggtgtacc gttggactgg
    12601 gttttcatct ccggtggatg gatgccgccg ttgaaatccc agattttatt ttttctgaat
    12661 gcagagaata acttaagcat ggtgttccac gggaatgata cgcacgggaa tggtgttcag
    12721 atcccatttc caggagtcag gtgtttctgc gaccggttgc aacgagatgc agtgcgtcgg
    12781 gcacggatca acacataaat tgcagcccgt acagagatca ctcattaccg tatgcatggc
    12841 acgggtagcg ccaacgatgg cgtctaccgg acacgcctga atacatttag tgcagccaat
    12901 acagttattt tcatcaataa ccgccaccat ccgcgcaggc gttatctctt gcgcttcgcc
    12961 atccagcggc tgcggctcga cattaagcaa ctcggcaatt tttagcatca cagcttcgcc
    13021 acctggggcg caacggttga ttttttcacc gttacagctg atggcttccg cgtaggggcg
    13081 acagccggga taaccgcact gaccacactg gctctgcggt aagatttcgt caattttctc
    13141 aacgaccgga tcgtcttcca ccgcaaaacg gcgggaggca taacccagaa tggcgccaaa
    13201 cgccaggccc agcaggctca cggcggcaac ggcaatccag atagcattca ttacaacttc
    13261 accaaaccac taaagcccat aaaggccaga gacataagac ctgcggtaat taacgcaatg
    13321 gcattaccgc gaaaaggtgc cgggacatca gccacagcaa ggcgttcgcg gatggcggcg
    13381 aagagcacca tcaccagcga gaaaccgaca gcggcggaaa aaccgtacag cgccgactgc
    13441 aagaaattgt gcccgagatt gatattcagc aacgccacgc cgagcactgc acagttggtg
    13501 gtgataagcg gcaaaaaaat ccccagcaga cggtaaagca ccgggctggt tttgcgcacc
    13561 accatctcgg tgaactgcac gaccacagca atcaccagaa taaatgccag ggtgcgcagg
    13621 taaatcagat taagtgggat caaaatccac gtatcgataa gccaggcgca aatagacgcc
    13681 agcgtcatca caaacgttgt tgccagcccc atgcccatcg cggtttccag ttttttggaa
    13741 acccccataa acggacagag accgagaaac ttgaccagta caaagttatt gaccagtaca
    13801 gttccgacaa acagtaacag gtagtcagtc atttttcggc ctgaaataaa aaagccgcct
    13861 attatccgtt aatccagagc aggcgacaac aggttaactg taaggttatt acggattcac
    13921 aaacgtcgct tttacccgcg acgagcgttt gaaatagggg acgatcaatg cggttgccaa
    13981 caaagtaaac agcaactggc gaacggctat gccatcttcc acgggtgaaa aagcgaatgc
    14041 ttttactgcc agcaatacgg aaataagcag ccagataata tagtgtttag gaacacaacg
    14101 ccgacgttta aagaaagcga tggtcaacca cagtgtgtaa taccacatgg cgatagcggt
    14161 aataaaagaa acgccccata agatttgtgt cgtcagtgct tgtccgccga gcgtttgaaa
    14221 tgtttgcgga gaagataacg cagcggtata cagcaacaac gccagcgtcg tactcaacag
    14281 agcgactaaa agccaggcca acgggccaag caaccagcct ccaatacgtt gtggcgttgt
    14341 ggtggtcata ggttctccag aatagcgacg caaacaggca aatctaattt taggggcgtt
    14401 attataaggc ttttataacc gatcgctact ttctttattg gacatagtgc cagacggact
    14461 tcggtacctg gccgaggtca aacaaacgcc cgccagaaac cagctctgcg cgacggtgat
    14521 cggcagcacg atacatattg atcagttcct gatcgtccgt cagggtgtaa ttaagatggt
    14581 cgtagagttt ttccagactt tcgagtgaac tgattttgcg aaattttaat aagtagtcct
    14641 gaacagtcat aaaaggtcca taaaa
//
LOCUS       DAJOSY010000103        14660 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJOSY010000103 DAJOSY010000000
VERSION     DAJOSY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.103"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(18..170)
                     /gene="hokE"
                     /locus_tag="OHN27_003449"
     CDS             complement(18..170)
                     /gene="hokE"
                     /locus_tag="OHN27_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCQ2287829.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            623..1741
                     /locus_tag="OHN27_003450"
     CDS             623..1741
                     /locus_tag="OHN27_003450"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCQ2287830.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1807..2055
                     /locus_tag="OHN27_003451"
     CDS             1807..2055
                     /locus_tag="OHN27_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCQ2287831.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVSTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2120..2488
                     /locus_tag="OHN27_003452"
     CDS             2120..2488
                     /locus_tag="OHN27_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCQ2287832.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2582..3235
                     /gene="nfsB"
                     /locus_tag="OHN27_003453"
     CDS             2582..3235
                     /gene="nfsB"
                     /locus_tag="OHN27_003453"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCQ2287833.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3343..4590
                     /gene="ybdG"
                     /locus_tag="OHN27_003454"
     CDS             3343..4590
                     /gene="ybdG"
                     /locus_tag="OHN27_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCQ2287834.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4658..6034)
                     /gene="pheP"
                     /locus_tag="OHN27_003455"
     CDS             complement(4658..6034)
                     /gene="pheP"
                     /locus_tag="OHN27_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCQ2287835.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6136..9279)
                     /gene="cusA"
                     /locus_tag="OHN27_003456"
     CDS             complement(6136..9279)
                     /gene="cusA"
                     /locus_tag="OHN27_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCQ2287836.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9291..10514)
                     /gene="cusB"
                     /locus_tag="OHN27_003457"
     CDS             complement(9291..10514)
                     /gene="cusB"
                     /locus_tag="OHN27_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCQ2287837.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10530..10862)
                     /gene="cusF"
                     /locus_tag="OHN27_003458"
     CDS             complement(10530..10862)
                     /gene="cusF"
                     /locus_tag="OHN27_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCQ2287838.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10886..12268)
                     /gene="cusC"
                     /locus_tag="OHN27_003459"
     CDS             complement(10886..12268)
                     /gene="cusC"
                     /locus_tag="OHN27_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCQ2287839.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12425..13108
                     /gene="cusR"
                     /locus_tag="OHN27_003460"
     CDS             12425..13108
                     /gene="cusR"
                     /locus_tag="OHN27_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCQ2287840.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13098..14546
                     /gene="cusS"
                     /locus_tag="OHN27_003461"
     CDS             13098..14546
                     /gene="cusS"
                     /locus_tag="OHN27_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCQ2287841.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3519 a         3968 c         3620 g         3553 t
ORIGIN      
        1 tactccccgc acaacggcta cttcttcggt tcgtaagcga gaacagcctt aaactcaata
       61 ttacgttcct tcaccgtaaa ctcacacagc gagtctccga ccagaagcgt aaatcccagc
      121 accgttaaac acagcactat gactgccgca agggcatatt tcgtcagcat gtttatattg
      181 cctccatgcg aagaagggac taccatccaa attgttcggg tttggagtgg cagccccagg
      241 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaaccg
      301 gaaagcttca ggcacccgcc gttatcttac ccttcctcaa caaaatagct gtttatttat
      361 acagtgagtc ctgtttcgga cttctcactc ctcatttaat caactgaagc tgaacccgtt
      421 tttttctgca tgactcaatc gtcaaaattc ttcgccacca gtcagcactg ccataacttg
      481 tcacattgca aaagtagcca aattgcttca ggcaccatca gttcaccatc accaaaaaaa
      541 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      601 aacgccaaca taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      661 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      721 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      781 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      841 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccacagacc atcatctgga
      901 aatttgcggc ggtggcacgc acccgtttca gaaatggcag cgtcaggagg tatgcgataa
      961 cgaacgctat caacgcacgc tggaaaactt tggttatctc attcagcagg cgaccgtttt
     1021 tggtcagcat gtccatgttg gctgcgccag tggcgatgac gccatttatt tgctgcacgg
     1081 cttgtcacga tttgtgccgc actttatcgc cctttccgcc gcgtcgccat atatgcaggg
     1141 aacggatacg cgttttgcct cctcacgacc gaatattttt tccgcctttc ctgataatgg
     1201 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctatttcgct gtttgagtta
     1261 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1321 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1381 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1441 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atgggctgga
     1501 aggcgtcatt accgatccgc acactggtga tcgtcgaccg ctaacggaag ataccttgcg
     1561 attgctggaa aaaatcgccc cttccgcaca taaaattggc gcatcgagcg caattgaagc
     1621 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1681 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1741 agtcgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1801 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1861 ttcctctctt gcctgctgct tcccgccccc gcattggggc tgacgctggc acaaaaactg
     1921 gtgagcacgt tccatctgat ggatcttagt cagctttaca ctttattgtt ttgtctgtgg
     1981 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     2041 tcgctggcgg attaaacgtc gattatcgcc gtacttcgcg gcatactttg cttattctcc
     2101 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctggc
     2161 ccttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     2221 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2281 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2341 gtatcacatg aataaaaagc actggatttc ggtgtatccc ggcgaggaaa tctccgaagc
     2401 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2461 cgatcaaaaa agagtgcgtc caggctaaag cggaaatcta tagcgcattt ttctcgctta
     2521 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttactttcac atggagtctt
     2581 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagtaa
     2641 aaaacttacc ccggaacagg ccgagcagat caaaactctc ctgcaataca gcccatccag
     2701 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2761 tgccaaatcc gctgccggta attatgtgtt caacgaacgt aaaatacttg atgcctcgca
     2821 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2881 ccaggaagat gctgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2941 tcgcaagttc ttcgctgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     3001 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     3061 cgcggtaccc atcgaaggtt ttgacgccgc catcctcgat gcagaatttg gtctgaaaga
     3121 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     3181 cgctacgctg ccgaaatcac gtctgccgca aaacatcacc ttaaccgaag tgtaattctc
     3241 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3301 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3361 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3421 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3481 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3541 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3601 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3661 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3721 cagaaattcc cggcagcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3781 atcggcgcga ttctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3841 attctgatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3901 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3961 tggctggaga tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     4021 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     4081 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     4141 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     4201 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4261 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4321 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgact
     4381 ttaatggtac gccaactggc tccaggtgat aatggtttac cgctcgagat ctatgcgttt
     4441 accaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4501 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4561 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4621 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4681 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4741 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4801 acggataaag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gctgcacgaa
     4861 aacgcagatg cgccagacag atcataatcc agttcaacag cagcgttgct accaccagcg
     4921 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4981 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     5041 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     5101 ccccgctgtt atacactgac agcgatgcta ccagaatgac gaagttcagc gcagaagcta
     5161 ccacgttgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     5221 ccacccacgg atagagcgcc agtaaaacca ccagtgaacc gatgtaaaac agcaggatgc
     5281 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5341 cagtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5401 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5461 ctttctcgcc gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5521 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5581 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5641 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5701 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5761 aggcaaagtg ggcaaatgaa ccggataccg gctcctcaac caccatttcg ccaagctggc
     5821 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5881 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccaacg
     5941 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     6001 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     6061 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag ccacaatcca cacggttaaa
     6121 cggggtatcc tgcttttatt tccgtacccg atgtcggtgc agccacatca gcttatacgc
     6181 cgccgggata ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggcgc
     6241 ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc
     6301 ggcgataatc accgccaccg tcatcgcttt cgggcgcacg cgcaggaccg cgccgtgata
     6361 taacgcctca tccagcttct gctcgctgaa tgtttgtgga ttattcaacg acggcacggc
     6421 ctctatggcg tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc
     6481 gagggcgata aagccagtgc ccgtcgccac ggaaagatga aagcccatcc accacagcaa
     6541 ccagatgccg cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac
     6601 ccgacggaac gccagataca acagcacgaa gataatcatc aacgtcatcg gcaccatgag
     6661 tttaagctta tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt
     6721 gcccggtttt aactgcactt tttcagctat cgctttttgt aaatcgtgaa ccaccgacac
     6781 catgtcacga tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat tctcggtttt
     6841 cagcatcgac ggtccggtag agactttaat gtcggccacg tctgccaggg tgatttgctg
     6901 cttcatcggc gtcaggatcg gcagctggcg cagtgcctgc ggactatcgc gccagctttg
     6961 cggataacgc agattaattg gataacgggc aatcccttcc accgtttcgc caaccatcgc
     7021 cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgtgc
     7081 ggctttttca cggttaatct caacgttgat atagcgccca ccttccagcc gctcggcaag
     7141 cgcagaagct acgcctggca ccgttcgcgc tacttcttca atttgctcag ccatcgcgtc
     7201 gatatccgcc agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga
     7261 gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagccg
     7321 cacggtgtta tccagttcct caatgatttt gtccatcgtc atgcctggcc gccactgctc
     7381 ctgcggctta agctggatgg tcgtttctac catctccagc ggagcagaat cggtggcggt
     7441 ttccgctttc ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt
     7501 gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg
     7561 catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag
     7621 cggccagaga accgtcagca ccgaaagcgc cgccaccagc agcgtggttt tcggccagtg
     7681 cagtactttc agcaacagcg gatgataaac acgaatcaaa aagcgattga gcgggttact
     7741 gctttccggc ggaattttgc cacggatcca gtagcccatc aggatcggga tcactacgat
     7801 cgccagcagc gccgcacccg ccatcgcata cgttttggtg aacgccaacg ggccaaacag
     7861 acgcccttcc tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag
     7921 actgataaat agcgccggcc ccacttcaac agacgcatcg gtgatcacct gccagcgcgt
     7981 tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc
     8041 attctcgatc atgacgatag cagcatcgac catcgccccg acggcaatcg caatgccacc
     8101 caacgacata atattggcat tcagtccctg gaagtgcatg acaataaaag caatacacaa
     8161 ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc
     8221 acagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcgatggc
     8281 gcggtcaatg agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact
     8341 acttttcagc gtttccagtt tgtccttcac ggcggcgatc acttctcggg cgtttttgcc
     8401 ggatcgcagg atcaccaccc cgcccgccac ttcgccttcg ccgtttagtt cggcaatgcc
     8461 ccggcgcatc tccgggccaa tctggacctt cgcaacatcg cgcagataaa cgggcacgcc
     8521 attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct
     8581 ggcgcgcacc atatattccg cttccgccag ttcgatcgac gaaccgcccg cttcctggtt
     8641 tgaagcatcc agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg
     8701 gggatcgata accacctgat actctttcac cacaccgccc accgacgcca cttccgcaac
     8761 gtcagggatg gttttcagct catatttgag aaaccagtcc tgtaatgagc gtaaatcggc
     8821 cagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt
     8881 ggcatctggc cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag
     8941 gtactccagc acccgcgaac gcgcccagta cggatcggtg ccatcttcga aaatgacata
     9001 cacataagag tcgccaaact gtgagaaacc gcgcacagtc ttcgcgccag gcaccgacaa
     9061 catggtggtg gttagcggat aagtcacctg attttcaacg atttgtggtg cctgaccggg
     9121 atagctggtt ttaataataa cctgcacatc ggagagatcc ggcagcgcat ccactggcgt
     9181 attaatgatg gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa
     9241 acggttcgcc accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca
     9301 tgggtagcac tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca
     9361 atcaggaaca ggccgctgga aaccaccttt tcaccttccg ccagaccaga gcgtaatgcg
     9421 gtgacgcctt gcgatgcctg gaaaacagca acgcgtttcg gtacaaagcg cccgtcggca
     9481 tcaacggtaa tcacccgctg ttcgctgccg gtatcaatca gcgcttgtga cggaatgagc
     9541 agcatcggtt cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc
     9601 tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca
     9661 ggtagcagcg tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagg
     9721 gtaaactgcg aggcatcttt caccagccag gcgatagact ccgggatcgc agcagtgacc
     9781 cacaccgggt ccataccctg aattttcgct accacgttat ctttggcgat attcattccc
     9841 gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg ctttgagcgt aaagcgagtc
     9901 tggatttttt gcgtggcgat caggcggcga atatccgcct ccggcattcc cgccagtcgc
     9961 agccgctcaa gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg cagcagtaaa
    10021 tactcactct gcgcttccac ccagtcagga atggtcaggt cgagaagcgg tgtgcccttt
    10081 tgtactttat cgcccacggt aagcggatac accttgtcga taaacccggc agcgcgagcc
    10141 tgcacaatgg catactgata ctcgttgtaa ctgacattcg ccgggaaact ctgggcaaaa
    10201 gtcagcggtc cgcgcgtgac ggtagccgtt ttcaccccca gattctgcgt ctgagtcggg
    10261 tcaatgcgca caccagacgc agaactctct tcatcggcat atttcggcac cagatccata
    10321 tccataaacg gcgatttacc tggtttatcg aaccgcgtat tgggatacat tgggtcgtac
    10381 cagaataaga ttttacgttc tgcggtcgac gttttttctg caggcggttc cgcctttgca
    10441 acccaggtaa aacctgccgc agaaataata ccgcccgcga tcatgctgcc gataataagc
    10501 gcgatttttt tcatctcatt aaacctgggt tactggctga ctttaatatc ctgtaataaa
    10561 gaaaggttgc cctgctggac aaaattaaac gccactttgt cgccggtttt aatttcactc
    10621 attttcgtct gcggggtgat ggtaaagcgc atggtcatct ccggccagtt cacagcagca
    10681 ataggatcgt gatggatggt gatttttttg ctttccagat caataccctt tacctcgcca
    10741 gtggcactaa taacctgtgg ttgtgcttcg ctcatggttt catgatgatg ttcgttagcc
    10801 tgggcattaa agccaataac ggtaaacaga ctgaacattg cgacttgcag tgcttttttc
    10861 atttattctc tcctggagtt aaaagttatt gctgccaacc gccaccaagt gcggtatata
    10921 aagaaatttc gttaacctga cgggcataat tcagatcaag taaagtttgt cgggttgcaa
    10981 ataaagaacg ctcggcatcc agcacttcca gataacttac tgcgccgtgc tgatataatg
    11041 cccgcgcccg ttgcaaagta atttgcagcg atgccagata acgctgctgg gcgctaattt
    11101 gatcgttcag gctttgacgt aatgccagcg catctgccac ttctttaaag gcgttctgga
    11161 ttttctgttc ataattcacc accgactgct gctggcgaat ttcggcgata tccagattgg
    11221 cctggttgcg tccggcatta aaaatgggga tctcaatttt gggtataaaa ttccacatcc
    11281 cgctgctggc gttaaataat gacgatagat cgctactggc ggtcgatatt ccgctggtca
    11341 ggcttataga cggaaaaaat gccgcgcgtg cagcgccaat attggcatta gccgccatta
    11401 acgcgtgttc agcttccata atatccgggc gctgcaataa gatttgcgac gacaatcccg
    11461 ccggtaattt aacgctttgc aggctgtcgc tgtttactgt ctgcgcttgc ggcagcttgc
    11521 cgtagcttcc cagcaacagt tgcaatgcat tattcgcctg cgccagttcc ccctgacgtt
    11581 tagcgatgtc gctgcgggta ctttctatca ccccgcgagc ctgttccagc gccagaacat
    11641 tactgctacc tgtcaacagt tgtttttcga caaacgcata tgactgctga taattacgca
    11701 gcgtttcttc ggctatttgc aattgcgcat acgccagttg ctgattgaaa tagctttgcg
    11761 cgacattaga aaccagcaga atatgcaccg cgcgctgagc ttcctcagtg gctaaataat
    11821 tttgtcgctc ggcttcgctc atgttcttta agcgaccgaa aaaatcgagg tcaaagctgg
    11881 cgttaaggcc ggtcgaaaac tcccgtgtcg tggcggtatt ccctttaaga ttaccgctcc
    11941 agctgccgct gccctcgcca ttgagctgtg ggtagcggtc ggcatcggtc agacgatatt
    12001 gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa atcccgatta ttcaccagcg
    12061 cctcgctaat cagcgtcttc acctgattat caacaaaaaa ggtgcgccag cccgcgttct
    12121 gatagttatc tgctgcgtta accaggccgt tctggctgag tgagaactgc tgcggcacgg
    12181 gcattgccgg acgctgataa tccggtgcca gtgaacaacc ggttagcgca agggccacac
    12241 aaaatggcag aagtttacaa ggagacatag gctcataatt tctggtgatt ttatgccgcc
    12301 aactttactc gccaggctct gattttccgg tgacagaaaa atgacaaaat tgtcattttg
    12361 ccaataagcg attgccatct gatcccgcta ctctagaatt gcccgggcaa catgcggagg
    12421 aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc ggagaatact tgaccaaagg
    12481 gttaaccgaa gccggttttg tggtcgattt ggccgacaac gggttgaatg gctaccatct
    12541 ggcgatgacc ggtgattatg atctgataat cctcgatatt atgctgccgg acgtgaacgg
    12601 ctgggatatc gtgcgcatgt tgcgttccgc caataaaggg atgccgattc tgttgcttac
    12661 cgcgcttggc accattgaac atcgcgtcaa ggggctggag ttgggggcgg atgactatct
    12721 ggtgaagccg ttcgcttttg ctgaactgct ggcgcgggtg cgcactctcc tgcggcgcgg
    12781 ggcggcggtg attatcgaaa gtcagtttca ggttgccgat ctgatggtcg atctcgtcag
    12841 ccgcaaagtc acccgcagcg gcacgcgcat cactctgacc agtaaagagt ttactctgct
    12901 ggagtttttc ctccgccatc agggcgaagt gctgccccgc tcgcttatcg cctcgcaggt
    12961 gtgggacatg aatttcgaca gcgacactaa cgccattgat gtagcggtga agcggctgcg
    13021 cggcaaaatc gacaacgact ttgagccgaa gctaattcag accgtgcgcg gcgtgggcta
    13081 catgcttgag gtgccggatg gtcagtaagc catttcagcg cccgttttcg ctggcaaccc
    13141 gcctgacctt ttttatcagc ctggccacca tcgcggcgtt tttcgccttt gcatggatca
    13201 tgatccactc agtaaaagtg cattttgccg agcaggatat taatgattta aaagagatta
    13261 gcgccacgct tgaacgggta cttaatcacc ctgacgaaac gcaagcccga cgcttaatga
    13321 cgctggaaga tatcgtcagt ggttattcca acgtgttgat ttccctggca gatagccacg
    13381 gcaaaacggt gtatcactcc cccggtgcgc ctgatatccg cgagtttgca cgtgatgcca
    13441 tacccgataa agacgcgcgg ggtggcgagg tgtatctcct ttccggcccg acgattatga
    13501 tgccaggcca cggtcacggg catatggaac acagcaactg gcggatgatt aacttgtcgg
    13561 ttggcccgtt ggtggacggc aaaccgattt atacgctcta catcgcgctt tcgatcgatt
    13621 ttcatcttca ttacataaat gatttgatga ataaacttat tatgaccgca tcggtaatca
    13681 gcatcctgat cgtctttatc gtactgttgg cggtacataa aggtcacgcg ccgatccgca
    13741 gcgtcagccg tcaaatccag aatatcacct cgaaagatct cgacgttcgc cttgacccgc
    13801 agaccgtgcc cattgagctg gaacagctgg tactgtcgtt caaccatatg atcgagcgta
    13861 ttgaggatgt ctttacccgc cagtccaatt tctcagcgga tatcgctcac gaaattcgca
    13921 cgccgattac caatctcatc acgcaaacgg aaatcgccct cagccagtct cgcagccaga
    13981 aggagctgga agatgtgctc tactctaatc tcgaagagct gacgcgaatg gcgaaaatgg
    14041 tcagcgatat gctgtttctc gctcaggccg ataacaacca gctaatcccc gaaaagaaaa
    14101 tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt tttcgaggcg ttagcggaag
    14161 atcgcggcgt ggagttgcga tttgttggcg acgaatgtca ggttgcgggc gatccgctga
    14221 tgctgcgtcg ggcgttaagc aacctgcttt ctaatgccct gcgttatacg ccgcctggag
    14281 aggcaattgt agtgcgctgc cagacggtcg atcaccaggt gcaagttacc gtcgaaaacc
    14341 ccggtacgcc cattgcgccc gagcacttac cgcgattgtt tgaccgtttc taccgcgttg
    14401 acccttcccg ccagcgaaaa ggcgaaggta gcggcattgg gctggcgata gtgaaatcga
    14461 ttgttgtcgc gcataaaggc acggttgcag taacgtcaga tgcgcggggg acaaggtttg
    14521 tgattatgct gccggagaga gagtgattat taagttcaaa ttcagggcgt ggtgcatcac
    14581 ctcctggtat tgttgatcaa caaattagag aaaaacttat taaacatatg aaatatcgaa
    14641 ggcttttatt tttcaaagtt
//
LOCUS       DAJOSY010000104        14575 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJOSY010000104 DAJOSY010000000
VERSION     DAJOSY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.104"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..632)
                     /gene="uxaB"
                     /locus_tag="OHN27_003462"
     CDS             complement(<1..632)
                     /gene="uxaB"
                     /locus_tag="OHN27_003462"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCQ2287842.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTL"
     gene            complement(859..1845)
                     /locus_tag="OHN27_003463"
                     /pseudo
     CDS             complement(859..1845)
                     /locus_tag="OHN27_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(1918..2277)
                     /locus_tag="OHN27_003464"
     CDS             complement(1918..2277)
                     /locus_tag="OHN27_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCQ2287843.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAAALPVNDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(2277..3203)
                     /gene="glsB"
                     /locus_tag="OHN27_003465"
     CDS             complement(2277..3203)
                     /gene="glsB"
                     /locus_tag="OHN27_003465"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCQ2287844.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(3267..4655)
                     /gene="sad"
                     /locus_tag="OHN27_003466"
     CDS             complement(3267..4655)
                     /gene="sad"
                     /locus_tag="OHN27_003466"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCQ2287845.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARHMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            4756..5637
                     /locus_tag="OHN27_003467"
     CDS             4756..5637
                     /locus_tag="OHN27_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2287846.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLDGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGYAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVIAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSARQGYQ"
     gene            5715..6829
                     /gene="yneK"
                     /locus_tag="OHN27_003468"
                     /pseudo
     CDS             5715..6829
                     /gene="yneK"
                     /locus_tag="OHN27_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            6979..8169
                     /gene="ydeA"
                     /locus_tag="OHN27_003469"
     CDS             6979..8169
                     /gene="ydeA"
                     /locus_tag="OHN27_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCQ2287847.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWIFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8194..8859)
                     /gene="marC"
                     /locus_tag="OHN27_003470"
     CDS             complement(8194..8859)
                     /gene="marC"
                     /locus_tag="OHN27_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCQ2287848.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9071..9505
                     /gene="marR"
                     /locus_tag="OHN27_003471"
     CDS             9071..9505
                     /gene="marR"
                     /locus_tag="OHN27_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCQ2287849.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9525..9908
                     /gene="marA"
                     /locus_tag="OHN27_003472"
     CDS             9525..9908
                     /gene="marA"
                     /locus_tag="OHN27_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCQ2287850.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9940..10158
                     /gene="marB"
                     /locus_tag="OHN27_003473"
     CDS             9940..10158
                     /gene="marB"
                     /locus_tag="OHN27_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCQ2287851.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10189..11088)
                     /gene="eamA"
                     /locus_tag="OHN27_003474"
     CDS             complement(10189..11088)
                     /gene="eamA"
                     /locus_tag="OHN27_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCQ2287852.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11283..12470
                     /gene="ydeE"
                     /locus_tag="OHN27_003475"
     CDS             11283..12470
                     /gene="ydeE"
                     /locus_tag="OHN27_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCQ2287853.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITVFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            12597..12692
                     /gene="mgtS"
                     /locus_tag="OHN27_003476"
     CDS             12597..12692
                     /gene="mgtS"
                     /locus_tag="OHN27_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCQ2287854.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(12911..13801)
                     /gene="dgcZ"
                     /locus_tag="OHN27_003477"
     CDS             complement(12911..13801)
                     /gene="dgcZ"
                     /locus_tag="OHN27_003477"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCQ2287855.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(14056..14448)
                     /gene="ydeI"
                     /locus_tag="OHN27_003478"
     CDS             complement(14056..14448)
                     /gene="ydeI"
                     /locus_tag="OHN27_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCQ2287856.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         3792 a         3725 c         3295 g         3763 t
ORIGIN      
        1 agcgtagaac agaagctgtt agcctgatcc agccattgaa taaatgcttc tggcagtgcc
       61 cactcttgcg catagcgcag caccagttca cgcaacgcgt cgccattgta gtcaatcaac
      121 tcacacggaa tgatgatcca acctttatcc agcgcaccgt tgaaatggct aaagcgttcg
      181 aacagcagac gggtcagttt tgccggatag cttaccgctg gcgcgtcatc gaatttatcg
      241 cccgcatggt agctgatacc cgcttcggtg gtgttagaga aaacaaagcg catttccggg
      301 ttgtgcgcca gtttcaggaa ttcatcgtat tcactgtaga cgctgatttc acgattaacc
      361 gagcgaatca gacgcgcgtc gctgaccgct tcccccttct cattcaggcc acggataatg
      421 gtggtgtaca gaccatcctg cgtgctcagt gacggcggga atgaagtttc aatcggacga
      481 acaacgacca cgccagaatt cagatcggtg tgctcattca ggagatcgat ttgccagtca
      541 acaaaggcgc gcaggaagtt accttcacca aactgaatga tacgttctgg atactgtgca
      601 ccgggaaaat cgcgacgatt tagtgttttc acaatgggtt cccttctgat tagtcataca
      661 acctgtttga attggtacga caggttagca aactttaata cgccgaaccc ctgttttgat
      721 caactcctga tgattaatga gcagttttat gagaaaagtg tggcgcggat catggtttaa
      781 tcgaggaaaa acgccttttc ctggatcata aagtggtaga acacattgca ttcaaatcgc
      841 gcgtaatgaa taaagatgtc agacaacttc ctcaccgtaa cgcatagtgc tggtacggtt
      901 gcgcccatct ttcttcgagc gatacagaca cgtatcggct tcgaccatca atttattaaa
      961 gtcatcggtc agcgttcggt acgatgcgcg accactaccg acgccaatac ttaccgtgag
     1021 ataaagcgtt ttttgttgcc aggtgaatgg ttgcagttca acgcctttac ggattttttc
     1081 cgccattagc agaccatcga caggattcac cgacggcact gcaacagcaa attcttcgcc
     1141 gcccattcgc gccaccagcc ccttatcacc gacaatcttc tgaatatgcc gggcaaacac
     1201 gcttaacact ttatcgccac attcatgtcc atagttatcg ttaatgcttt tgaaatagtc
     1261 gatatcaagc agcatgacag tcagatgttg tgtctgtttc agcgatggac ttttcagcgc
     1321 ctcataaaga ccggaccgtg agtaaacctg agtcagaaaa tcaaagtcgg ctcgcagcgc
     1381 aacttgcttc attagcgaat tgatcgctgc cacgctaaaa gaaaccataa ttgggcatat
     1441 cgccatcgtg gcaataccga gacgtgcgga gaacatttgc ggaatggaga acggcgaacc
     1501 gaccgagata tcaatcaccg aatttgccac cagtacgatt tccaccgcac cggtgacaaa
     1561 ggtcaacaga catgttacct gcggcgtata gcgcactgca caccagatta atgcaggcag
     1621 cgggaacgcc agactccccg ctccgccaat gactaccgag gcaataaccg acacaataag
     1681 cgcgatggcg ggcatcatct gttctgcttt aaagcgcggc agcactccag gaatagccaa
     1741 cgtcagcata caaggcacga tcaacacgcc cgttgagaat tgctcactga accagtccgc
     1801 aagcaaaggc cagaaatcca gactatcaat actgaccgaa ccaagatagc atgttcgctg
     1861 acagcggcgg caaataaagg taccctggga atgctatttt gtcgttttcc gcaattctca
     1921 tggctgattc atctgtacaa ccagccagtg atagattacc gccacgatat aacgttgttg
     1981 ttcttcgctt aacgaaacac cctgcggaat gttatgccac ttagccagac aaccgcgaca
     2041 gcaggtcgcg gtggcgtgct gagcgataaa caccggatgc ccgcgcatgg gggtttgctt
     2101 accgtcatta accggtaatg ccgcagcaag acgtttagcg acaaaatccg ccgcatgttg
     2161 ttcgatgact ggcgcgcctt tctccagaca atactgacgc tctttcatgc ccaaacgaaa
     2221 gcgagagcga aattttgaac gggataaacg cgcgaagaga ggatcgagag actgcattaa
     2281 taaaccgaac gccctaactg tttcgtcaat tgttcaagga cggcaatgcc cgcaagcgag
     2341 ttacctgcat catccagttc cggactccag actgcgatgg ccatttcatg cggaacaatc
     2401 gccacaatac cgcccccaac gccagatttc gccggtagtc ccacccgcca ggcaaactcc
     2461 cccgcgttct ggtacatacc actggtcgcc atcagcgcgt taatttgccg cgcctgcatt
     2521 ggcgtcacca ctggttcatc aatatgaata gctttcccct gattagccag aaagacaaac
     2581 gtccgggcca gctctacaca gctcattttc agagcgcagt aatgaaagta gttttgcaga
     2641 acggttgtca cgtcatgatg gaaattgcca aacgacttca tcagccaggc gatagccgca
     2701 tttcgcgcgg aatgttcaaa ttcggaacgc gctaccaccg tatcgtagga aatatcagac
     2761 acaccgctta agccgcgcac gacttccagc atacgttgcc gtggtgcgct taatcgccct
     2821 tgcaacatat cgcagaccac cagcgcaccg gcattaatga acggattacg cggtataccc
     2881 tgctccattt ccagttgcac taaggaattg aacggtgatc cagacggatc tttgccgacg
     2941 cgttgccaga tttcctcttc ggagtaatga cgcatggcga caacgagact cagcactttg
     3001 gaaatagact gaatggaaaa acgttcttgc gcgtctccgg cctgaaaaag ctgtccgtca
     3061 acggtacaga tagcaatccc caatcgggaa ccgtctactg tagccagcgc cggaatataa
     3121 tccgcgactt taccctgacc aatgagcggc cgcacttgcc gcaagatgtt ttctaaaatt
     3181 gcattatcca tggcgactgc cactttctac tcctggaccg caggtctgaa aagacctgcg
     3241 agtatatcag agctgaatat gtcgcgtcag atccggtctt tccacaccgt ctggatatta
     3301 cagaattcgt gtaagccgaa atgggaaagc tcacgaccaa agccactctt tttcacgcca
     3361 ccaaaggcca ctcgcgcgtc gctggcacaa taaccattga taaacacccc accgcattcc
     3421 agacgtgccg ccatatgtct ggcctgtgtt tcgtcagtgg taaaaatggt cgctgaaagg
     3481 ccgaactcac tatcattagc cagttccagt gcatgttctg catctttcgc aatggtgatt
     3541 gccgcaacgg ggccaaacat ttcttcccga aacgcggtca tttctggggt aacattcgcc
     3601 agaaccgttg gcggatagta gttacctgcc ccagccatct tttccccgcc cagtaacaaa
     3661 cgcgcaccct gcgccagggt tttctccacc tgatgatgca gctcatcacg taaatcaaaa
     3721 cgagccattg gtccgagagc gttctcttcg tcacggggat cgcccatttt caaggctgcc
     3781 gcagctgcca caaaacgttc ggtaaatgcc gaagcaattc cctcttcgat aataaagcgt
     3841 tttgccgctg cacatacctg tccggtattc tgataacgtc cggctaccgc cgctttcacc
     3901 gccagttcca gatcggcatc gttaagcaca ataaacggat ccgaaccgcc cagttccagt
     3961 acgcattttt tcagtgccgc tccagcctgt gcgccaatag ccgctcccgc acgaacactt
     4021 ccggtcaccg tgacagcagc aatgcgcgag tctttaatca tctgactgac accgtcgttg
     4081 tcggcattca gccagccata tacgccttgt gggatacccg catctttaaa cacctgggca
     4141 atgagctgtg cacagcccat cacattcggc gcatgtttaa gtaagtagcc gttacctgca
     4201 agaatgatgg gaacagcgcc acgcatcacc tgccataacg gaaaattcca cggcataatc
     4261 gccagaatcg tccccaacgg tcgatactca ataaccgcct gctgattttc caccagcgta
     4321 ggttccgcct tcagcattgc cggaccatgt tctgcatacc agtcacacaa attcgccgat
     4381 ttcgccactt cagcgcgcgc ctggttgatt ggtttgccca tttcgcgggt gatcatttgc
     4441 gccatttctt cgctacgagc gcgcagagcc ttaccgatat cacgcagttt ttcagcacga
     4501 taatctatat ttgtctcgcg ccagtcgcga aagcctgctg ccgccagctg aagtgcgttt
     4561 tcgatatcgt cagcgccagc ccacggcagc acagaaagtt gttcacccgt ggcaggattt
     4621 atcgaaattg catgagttgc cggagtaatg gtcatcgggg tatctccttt atgagtcatg
     4681 gtatgaagat acgcagattt actcttgctt taaaatgaat aatattaagc cacttattca
     4741 cgaatcgaga atgctatgga tctgacccaa ctggagatgt tcaacgcggt tgccgaagct
     4801 ggcagcataa cccaggctgc agcaaaagtg catcgcgtgc cgtcgaatct cactacccgt
     4861 ctgcgccagc tggaaacaga actgggggtt gatctgttta ttcgcgagaa tcagcgttta
     4921 cgtctctctc ctgccgggca taacttttta cgctatagcc aacaaattct cgcgttagtg
     4981 gatgaagcgc ggagcgttgt cgctggcgat gagccgcaag gtttattttc tcttggttcg
     5041 ctggaaagca ccgctgcagt gcgcattcca gccacgctgg cggaattcaa ccgtcgttat
     5101 cccaaaattc agttttcact ttccaccggc ccttccggca ctatgctgga tggtgtactg
     5161 gagggaaaac tgaatgcggc gtttattgat ggacccatta accatactgc catcgacggg
     5221 ataccggtat accgcgagga actgatgatc gtcacgccac aaggatatgc gccagtaacc
     5281 cgtgccagtc aggttaatgg cagtaacatt tatgccttcc gcgccaattg ttcgtatcgt
     5341 cgccacttcg agagctggtt tcatgctgac ggtgccgctc cgggaactat ccatgagatg
     5401 gagtcttatc acggaatgtt ggcctgtgtg atcgcaggag caggcattgc gcttattccg
     5461 cgctctatgc tggaaagtat gccggggcat caccaggttg aagcgtggcc gttagctgag
     5521 caatggcgtt ggttaacaac ctggctggtc tggcgtcgtg gtgcgaaaac gcgcccgctc
     5581 gaggcattta ttcaactgct ggatgtgcct gactcggcaa gacagggata tcaatgagct
     5641 atttttgata gttcttgcgg ctaatatgct ctatatagtg atgttccgat gacttatgac
     5701 tatatggggc aaatatggtt acgccagtaa gcatcagcaa ttacatatct cttcctgatg
     5761 attttcccgt acgtaatatt gcacctcagg taaaagaagt tttaaaggat tttattgatg
     5821 cactcagtac cataatatgt gatgaagagt ggcgcacgtc tttaaacatc aactcagcca
     5881 caaaaaagat atttaataac cttgacaact tatcatatat tcagaggact tcttttcggg
     5941 gtaacgacac actatacaat gaaaaggttc agtttaaact cacttatccc gtcaagaatg
     6001 gaagacacaa agaaaatatt gaatttcagg tagtaataaa tttaatccct atttatttag
     6061 ataattttcg ccatgatgga gaaattaata tttttgcgct cccaacccaa agcctgtcac
     6121 tatggggcgc gtatttcaga ccggcgtcga gcgtgtgctc tttctgtttc tgaatgattt
     6181 tattgaacaa tttccaatga tcaaccttgg cgttcccatc aaaagagcgc atacaccaca
     6241 tattgaaccc ctgcctccgg atcaccatac cgctgcagat tacttacgcc agtttgattt
     6301 gcttgtcctg aattttatct ctcgcggtaa ttttgtcata ctcccccgat tatggagtaa
     6361 ctctgaggtt cacagatggt tcgtcaataa agatcctaac ttgatcaccg ccattctcga
     6421 cataacagac ggcgaattaa aagaggattt attgcaaagc ctgatggatt cattaggttc
     6481 taacaaacat gtactacccg aagtctgcat ctgcttttta tcccttttag cagaacaaga
     6541 atctcctcat tttcaggact tttttttatt tttcgccaat atgttattgc actatcacca
     6601 atttatgaat cccaatgaaa gtgatctgaa tgacgtgttg atgccagcat cattaagtga
     6661 tgataaaatt atcaaacata tggcgcgcag gaccctcaaa ctgtttgtaa aaaatgaaac
     6721 gccgccaaaa gttactcacg aagacctggt gaaaaacagg cctcgctccc ctgtcagacc
     6781 acctataccc gcaaccgcca aaacgccaga cctccctgaa cgtcattaaa ccgtgatgtt
     6841 accgactctc tgacgcggaa cagaatcagc gtcagagaaa cggaaaacgc gatccagatc
     6901 acaaatgcat tgtattcaca tcattaaccg ttttaagatc atttcatcac tttttcgcaa
     6961 ctcacccgat aatctgttat gacaacaaac actgtttccc gcaaagtggc gtggctacgg
     7021 gtcgttacgc tggcagtcgc cgccttcatc ttcaacacca ccgaatttgt ccctgttggc
     7081 ctgctctctg acattgcgca aagttttcac atgcaaaccg ctcaggtcgg catcatgttg
     7141 accatttacg catgggtagt agcgctaatg tcattgcctt ttatgttaat gaccagtcag
     7201 gttgaacggc gcaaattact gatctgcctg tttgtggtgt ttattgccag ccacgtactg
     7261 tcgtttttat cgtggatctt taccgttctg gtgatcagtc gcattggtgt ggcttttgca
     7321 catgcgattt tctggtcgat tacggcgtct ctggcgatcc gtatggctcc ggccgggaag
     7381 cgggcacagg ccttgagttt aattgccacc ggtacagcac tggcgatggt cttaggttta
     7441 cctctcgggc gcattgtggg gcagtatttc ggttggcgaa tgaccttctt cgcgattggt
     7501 attggggcgc ttatcaccct tttgtgcctg attaagttac ttcccttact gcccagtgag
     7561 cattccggtt cattgaaaag cctcccgcta ttgttccgcc gcccggcatt gatgagcatt
     7621 tatttgttaa ctgtggtggt tgtcaccgcc cattacacgg catacagcta tatcgagcct
     7681 tttgtgcaaa acattgcggg attcagcgcc aactttgcca cggcattact gttattactc
     7741 ggtggtgcgg gcattattgg cagcgtgatt ttcggtaaac tgggtaatca gtatgcgtct
     7801 gcgttggtga gtacggcaat tgcgctgttg ctggtgtgtc tggcactgct gctacctgcg
     7861 gcgaacagtg aaatacacct cggggtgctg agtattttct gggggatcgc gatgatgatc
     7921 atcgggcttg gtatgcaggt taaagtgctg gcgctggcac cagatgctac cgacgtcgcg
     7981 atggcgctat tctccggcat atttaatatt ggaatcgggg cgggtgcgtt ggtaggtaat
     8041 caggtgagtt tgcactggtc aatgtcgatg attggttatg tgggcgcggt gcctgctttt
     8101 gccgcgttaa tatggtcaat cattatattt cgccgctggc cagtgacact cgaagaacag
     8161 acgcaatagt tgaaaggccc attcgggcct tttttaatgg tacgttttaa tgatttccag
     8221 gatgccgtta ataataaact gcacacccat acataccagc aggaatccca tcagacggga
     8281 gatcgcttca atgccaccct tgcccaccag ccgcataatt gcgccggagc tgcgtaggct
     8341 tccccacaaa ataaccgcca ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca
     8401 atcagcgaag gttgaactct gacgcactgt ggacgccgag ctaataatca tcgctatggt
     8461 tcccggacca gcggtacttg gcattgctag cggcacaaag gcaatattgg cactgggttc
     8521 atcttccagc tcttccgact tgcttttcgc ctccggtgaa tcaatcgctt tctgttgcgg
     8581 aaagagcatc cgaaaaccga taaacgcgac gattaagccg cctgcaattc gcagaccggg
     8641 aatcgaaatg ccaaatgtat ccatcaccag ttgcccggcg taatacgcca ccatcatgat
     8701 ggcaaatacg tacaccgagg ccatcaacga ctgacgatta cgttcggcac tgttcatgtt
     8761 gcctgccagg ccaagaaata acgcgacagt tgttaatggg ttagctaacg gcagtaacac
     8821 caccagcccc aggccaattg ctttaaacaa atctaacatt ggtggttgtt atcctgtgta
     8881 tctgggttat cagcgaaaag tataaggggt aaacaaggat aaagtgtcac tctttagcta
     8941 accttgcatc gcattgacca aaacttgaac cgatttagca aaacgtggca tcggtcaatt
     9001 catttatttg acttatactt gcctgggcaa tattatcctc tgcaactaat tacttgccag
     9061 ggcaactaat gtgaaaagta ccagcgatct gttcaatgaa attattccat tgggtcgctt
     9121 aatccatatg gttaatcaga agaaagatcg tctgcttaac gagtatctgt ctccgctgga
     9181 tattaccgcg gcacagttta aggtgctctg ctctatccgc tgcgcggcgt gtattactcc
     9241 ggttgaactg aaaaaagtgt tgtctgtcga ccttggagca ctgacccgta tgctggatcg
     9301 cctggtctgt aaaggctggg ttgaaaggtt gccgaacccg aatgataagc gcggcgtact
     9361 ggtaaaactt accaccagcg gcgcggcaat atgcgaacaa tgccatcaat tagttggcca
     9421 ggacctgcat caagaattaa caaaaaacct gacggcggac gaagtggcaa cacttgagca
     9481 tttgcttaag aaagtcctgc cgtaaacaaa aaagaggtat gacgatgtcc agacgcaata
     9541 ctgacgctat taccattcat agcattttgg actggatcga ggacaacctg gaatcgccac
     9601 tgtcactgga gaaagtgtca gagcgttcag gttactctaa atggcacctg caacggatgt
     9661 ttaaaaaaga aaccggtcat tcattaggcc aatacatccg cagccgtaag atgacggaaa
     9721 tcgcgcaaaa gctgaaggaa agtaacgagc cgatactcta tctggcagaa cgatatggct
     9781 tcgagtcgca acaaactctg acccgaacct tcaaaaatta ctttgatgtt ccgccgcata
     9841 aataccggat gaccaatatg cagggcgaat cgcgcttttt acatccatta aatcattaca
     9901 atagctagtt gataacgtga caacgtcact gaggcaatca tgaaaccact ttcatccgca
     9961 atagcagctg cgcttattct cttttccgcg cagggcgttg cggaacaaac cacgcagcca
    10021 gttgttactt cttgtgccaa tgtagtggtt gttcccccat cacaggaaca accaccgttt
    10081 gatttaaatc acatgggtac tggcagtgat aagtcggatg cgctcggcgt gccctattat
    10141 aatcaacacg ctatgtagtt tgttctggcc ccgacatctc ggggcttatt aacttcccac
    10201 ctttaccgct ttacgccacc gcaagccaaa tacattgata tacagcccgg tcataatgag
    10261 caccgcacct aaaaattgca gacccgttaa gcgttcatcc aacaatagtg ccgcacttgc
    10321 cagtccgact acgggcacca gtaacgataa cggtgcaacc cgccaggttt catagcgtcc
    10381 cagtaacgtc ccccagatcc cataaccaac aattgtcgcc acaaacgcca gatacatcag
    10441 cgacaagatg gtggtcatat caatagtaac cagactgtga atcatggatg cggaaccatc
    10501 gagaatcagc gaggcaacaa agaagggaat gattgggatt aaagcgctcc agattaccag
    10561 cgacatcacc gccggacgcg ttgagtgcga catgatcttt ttattgaaga tgttgccaca
    10621 cgcccaacta aatgctgcag ccagggtcaa cataaagccg agcatcgcca catgctgacc
    10681 gttcagacta tcttcgatta acaccagtac gccaaaaatc gctaaggcga tccccgccaa
    10741 ttgtttgcca tgcagtcgct ccccgaaagt aaacgcgcca agcacgatag taaaaaacgc
    10801 ctgtgcctgt aacaccagcg aagccagtcc agcaggcata ccaaagttaa tggcgcagaa
    10861 cagaaaggca aactgcgcaa aactgatggt taatccatac cccaagagca aattcagtgg
    10921 tactttcggt cgtgcaacaa aaaagatggc cggaaaagcg accagcataa agcgcaaacc
    10981 ggccagcatc agcggtggca tgttatgaag ccccactttg atgaccacaa aatttagccc
    11041 ccatacgacc actaccagta gcgccaacac accatctttt cgcgacattc taccgcctct
    11101 gaatttcatc ttttgtaagc aatcaactta gctgaattta cttttcttta acagttgatt
    11161 cgttagtcgc cggttacgac ggcattaatg cgcaaataag tcgctatact tcggattttt
    11221 gccatgctat ttctttacat ctctaaaaca aaacataacg aaacgcactg ccggacagac
    11281 aaatgaactt atccctacga cgctctacca gcgcccttct tgcctcgtcg ttgttattaa
    11341 ccatcggacg cggcgctacg ctgccattta tgaccattta cttgagtcgc cagtacagcc
    11401 tgagtgtcga tctaatcggt tatgcgatga caattgcgct cactattggc gtcgttttta
    11461 gcctcggttt tggtatcctg gcggataagt tcgacaagaa acgctatatg ttactggcaa
    11521 ttaccgtctt cgccagcggt tttattgcca ttcctttagt gaataacgtg acgctggttg
    11581 tgctcttttt tgccctcatt aactgcgcct attctgtttt tgctaccgtg ctgaaagcct
    11641 ggtttgccga caatctttcg tccaccagca aaacgaaaat cttctcaatc aactacacca
    11701 tgctaaacat tggctggacc atcggtccgc cgctcggcac gctgttggtg atgcagagca
    11761 tcaatctgcc cttctggctg gcagctatct gttccgcgtt tcccatgctt ttcattcaaa
    11821 tttgggtaaa gcgcagcgag aaaatcatcg ccacggaaac aggcagtgtc tggtcgccga
    11881 aagttttatt acaagataaa gcactgttgt ggtttacctg ctctggtttt ctggcttctt
    11941 ttgtaagcgg cgcatttgct tcatgcattt cacaatatgt gatggtgatt gctgatgggg
    12001 attttgccga aaaggtggtc gcggttgttc ttccggtgaa tgctgccatg gtggttacgt
    12061 tgcaatattc cgtgggccgc cgacttaacc cggctaacat ccgcgcgctg atgacagcag
    12121 gcaccctctg tttcgtcatc ggtctggtcg gttttatttt ttccggcaac agcctgctat
    12181 tgtggggtat gtcagctgcg gtatttactg tcggtgaaat catttatgcg ccgggcgagt
    12241 atatgttgat tgaccatatt gcgccgccag gaatgaaagc cagctatttt tccgcccagt
    12301 ctttaggctg gcttggtgcc gcgattaacc cattagtgag tggcgtagtg ctaaccagcc
    12361 tgccgccttc ctcgctgttt gtcatcttag cgttggtgat cattgctgcg tgggtgctga
    12421 tgttaaaagg gattcgagca agaccgtggg ggcagcccgc gctttgttga tttaagtcga
    12481 acacaataaa aatttaattc agccttcgtt taggttacct ctgctaatat ctttctcatt
    12541 gagatgaaaa ttaaggtaag cgaggaaaca caccacacca taaacggagg caaataatgc
    12601 tgggtaatat gaatgttttt atggccgtac tgggaataat tttattttct ggttttctgg
    12661 ccgcgtattt cagccacaaa tgggatgact aatgaacgga gataatccct cacctaaccg
    12721 gccccttgtt acagttgtgt acaaggggcc tgatttttat gacggcgaaa aaaaaccgcc
    12781 agtaaaccgg cggtgaatgc ttgcatggat agatttgtgt tttgctttta cgctaacagg
    12841 cattttcctg cactgataac ggatcgttga cacagtagca tcagttttct caatgaatgt
    12901 taaacggagc ttaaactcgg ttaatcacat tttgttcgtc aataaacatg cagcgatttc
    12961 ttccggtttg cttaccctca tacattgccc ggtctgctct tccaatgacc acatccagag
    13021 gctcttcagg aaatgcgcga ctgacacctg ctgtcacggt aatgttgata tgcccttcag
    13081 aatgtgtgat ggcatggtta tcgactaact ggcaaattct gacacctgca cgacatgctt
    13141 cttcatcatt agccgctttg acaataatga taaattcttc gcccccgtag cgataaaccg
    13201 tttcgtaatc acgcgtccaa ctggctaagt aagttgccag ggtgcgtaat actacatcgc
    13261 cgattaaatg cccgtaggta tcattaacca atttaaatcg gtcaatatcc aacaacatta
    13321 aataaagatt cagaggctca gtgttgcgta actgatgatc aaaggattca tcaagaaccc
    13381 gacgccccgg caatcccgtc aaaacatcca tattgctacg gatcgtcagc aaataaattt
    13441 tgtaatcggt taatgccgca gtaaaagaaa gcaacccctc ctgaaaggcg tcgaaatgcg
    13501 cgtcctgcca gtgattttca acaatagcca gcattaattc ccgaccacag ttatgcatat
    13561 gttgatgggc agaatccatt agccgaacgt aaggtaattc atcgttatcg agtggcccca
    13621 gatgatcaat ccaccgacca aactggcaca gtccataaga atggttatcc gttatttctg
    13681 gcttactggc atctctcgcg accacgctgt gaaacatact caccagccac tggtagtggg
    13741 catcgatagc cttattgaga tttaacaaga tggcatcaat ttccgttgtc ttcttgatca
    13801 ttgccactcc tttttcacag ttccttgtgc gcgctattct aacgagagaa aagcaaaatt
    13861 acgtcaatat tttcatagaa atccgaagtt atgagtcatc tctgagataa cattgtgatt
    13921 taaaacaaaa tcagcggata aaaaagtgtt taattctgta aatcacctct gcattaccgt
    13981 aaataaaagg atgacaaata gcataaccca ataccctaat ggcccagtag ttcaggccat
    14041 caggctaatt tatttttatt tctgcaaatg agtgacccga acgacggccg gcgcgctttt
    14101 cttatccaga ctgccgctaa tgttgatcat ctggtccggc tgaacttctc gtccatcaaa
    14161 gacggccgca ggaataacga cattaatttc accgctctta tcgcgaaaaa cgtaacggtc
    14221 ctctcctttg tgagaaatca aattaccgcg tagtgaaacc gaagcgccat cgtgcatgtt
    14281 ttttgcgaaa tcaacggtca ttttttttgc atcatcggtt ccgcgatagc catcttctat
    14341 tgcatgaggc ggcggtggcg ctgcatcctg ttttaaaccg ccctggtcat ctgccaacgc
    14401 ataaggcatg acaagaaaac ttgctaatac aatggcctga aatttcatac taactcctta
    14461 attgcgtttg gtttgactta ttaagtctgg ttgctatttt tataattgcc aaataagaat
    14521 actgccaatt gttataaggc atttaaaatc agcccactag ctgtcaaata ttcag
//
LOCUS       DAJOSY010000105        14452 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJOSY010000105 DAJOSY010000000
VERSION     DAJOSY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.105"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            66..1355
                     /locus_tag="OHN27_003479"
     CDS             66..1355
                     /locus_tag="OHN27_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCQ2287857.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGCLVNRIVSEETAK"
     gene            1352..2818
                     /gene="hpaE"
                     /locus_tag="OHN27_003480"
     CDS             1352..2818
                     /gene="hpaE"
                     /locus_tag="OHN27_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCQ2287858.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2820..3671
                     /gene="hpaD"
                     /locus_tag="OHN27_003481"
     CDS             2820..3671
                     /gene="hpaD"
                     /locus_tag="OHN27_003481"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCQ2287859.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3681..4061
                     /locus_tag="OHN27_003482"
     CDS             3681..4061
                     /locus_tag="OHN27_003482"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCQ2287860.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4126..4929
                     /gene="hpaH"
                     /locus_tag="OHN27_003483"
     CDS             4126..4929
                     /gene="hpaH"
                     /locus_tag="OHN27_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCQ2287861.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4940..5728
                     /gene="hpaI"
                     /locus_tag="OHN27_003484"
     CDS             4940..5728
                     /gene="hpaI"
                     /locus_tag="OHN27_003484"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCQ2287862.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPDVY"
     gene            5903..7279
                     /gene="hpaX"
                     /locus_tag="OHN27_003485"
     CDS             5903..7279
                     /gene="hpaX"
                     /locus_tag="OHN27_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCQ2287863.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7289..8179
                     /gene="hpaA"
                     /locus_tag="OHN27_003486"
     CDS             7289..8179
                     /gene="hpaA"
                     /locus_tag="OHN27_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCQ2287864.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8430..9992
                     /gene="hpaB"
                     /locus_tag="OHN27_003487"
     CDS             8430..9992
                     /gene="hpaB"
                     /locus_tag="OHN27_003487"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCQ2287865.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10010..10522
                     /locus_tag="OHN27_003488"
     CDS             10010..10522
                     /locus_tag="OHN27_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCQ2287866.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10915..13065
                     /gene="btsT"
                     /locus_tag="OHN27_003489"
     CDS             10915..13065
                     /gene="btsT"
                     /locus_tag="OHN27_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCQ2287867.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13115..13318
                     /locus_tag="OHN27_003490"
     CDS             13115..13318
                     /locus_tag="OHN27_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCQ2287868.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13329..14285
                     /gene="yjiA"
                     /locus_tag="OHN27_003491"
     CDS             13329..14285
                     /gene="yjiA"
                     /locus_tag="OHN27_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCQ2287869.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(14354..>14452)
                     /locus_tag="OHN27_003492"
     CDS             complement(14354..>14452)
                     /locus_tag="OHN27_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCQ2287870.1"
                     /translation="TKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         3339 a         3954 c         3964 g         3195 t
ORIGIN      
        1 caatcgggaa taaaagtaat cattaacata ttaatgatta agatgatcga atccgaggag
       61 tggtaatgaa aggcactatc ttcgccgtag cgttgaacca tcgcagccaa cttgatgcat
      121 ggcaggaagc gttccagcaa tccccctaca aagccccgcc taaaactgcg gtctggttta
      181 ttaaaccgcg caatacggtg attggttgcg gtgaaccgat tccctttcca cagggtgaaa
      241 aggtactgag cggtgcgact gttgcgctga ttgtgggaaa aacggcgacg aaagtacgtg
      301 aagaagatgc ggcagagtac atcgccggat atgcgctggc taacgacgtc agcctgccgg
      361 aagagagctt ttaccgcccg gcaatcaaag caaaatgtcg tgatggattc tgccccattg
      421 gcgaaaccgt ggctctcagc aatgtcgata atctgaccat ctataccgag atcaacgggc
      481 gtcctgccga tcactggaat accgccgatt tacaacgtaa cgccgcgcag ttgctgagcg
      541 ccctgagcga atttgccaca ctgaatccag gcgatgccat tctgctcggc acgccacagg
      601 cgcgcgtgga aatacagcca ggcgatcgcg ttcgtgttct cgcagaaggt ttcccgccgc
      661 tggaaaatcc ggtagtggac gaacgtgaag tgaccacgcg caagagcttc ccaacgcagc
      721 cacacccgca cggcacgctg tttgccctcg gcctgaacta cgccgaccac gccagcgaac
      781 tggaatttaa gccaccggaa gaaccgctgg tgttcctgaa agcgccaaat accctcactg
      841 gcgataacca gacctccgtg cgtccaaaca atattgaata catgcactat gaagcggagc
      901 tggtggtagt tattggcaaa caggcgcgta acgtcagcga agccgatgcc atggattatg
      961 tcgcgggcta caccgtgtgt aacgactacg ccattcgcga ctatctggaa aactactacc
     1021 gccctaacct gcgggtaaaa agccgcgacg gactgacgcc gatgctttca accatcgtgc
     1081 cgaaagaggc gatcccggac ccgcataatc tgacccttcg caccttcgtc aacggcgagt
     1141 tacgccagca aggcaccacc gccgatctga tcttcagcgt gcccttcctg atcgcctact
     1201 taagcgaatt tatgaccctg aatccgggcg acatgatcgc caccggcaca ccaaaaggct
     1261 tatctgacgt ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg ggctgcctgg
     1321 tgaaccgaat tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg
     1381 caaaaatgtt gcaggtaacg actacttcca gaccaccaat ccggcgacgg gtgaagtgct
     1441 ggcggatgtg gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga
     1501 ggcgttcccg aaatgggcca atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct
     1561 gggcgatctg atcgaccaga acgtgcctga gatcgccgcg atggaaaccg cggacacggg
     1621 cctgccgatc catcagacca aaaatgtgtt gatcccacgc gcttctcaca actttgaatt
     1681 tttcgcggaa gtctgccagc agatgaacgg caagacctat ccggttgacg acaagatgct
     1741 caactacacg ctggtgcaac cggtaggcgt ttgtgcgctg gtatcgccgt ggaacgtgcc
     1801 gtttatgacc gccacatgga aggtcgcgcc gtgtcttgcg ctgggcaata ctgcggtgct
     1861 gaagatgtca gaactctccc cgctgaccgc tgaccgcctg ggtgagctgg cgctggaagc
     1921 cggtattccg gcgggcgtgc tgaacgtggt acagggctac ggcgcaaccg caggggatgc
     1981 gctggtccgt catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg
     2041 caacatcatg aaaaacgccg ggctgaaaaa atactccatg gaactgggcg gaaaatcgcc
     2101 agtgctgatt tttgaagatg ccgatattga acgcgcgctg gacgccgccc tgttcaccat
     2161 cttctcgatc aacggcgagc gctgcaccgc cggttcgcgc atctttattc agcaaagcat
     2221 ctacccggaa ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc
     2281 gaacgatccg aatacccagg ttggggcact tatcagccag caacactggg aaaaagtctc
     2341 cggctatatc cgtctcggca ttgaagaagg cgcaaccctg ctggcgggcg gcccggataa
     2401 accgtctgac ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc
     2461 agacgtagat aaccgtatgc gcgtcgccca ggaagagatt ttcgggccgg tcgcctgcct
     2521 gctgccgttt aaagacgaag ccgaaggctt acgcctggca aacgacgtgg agtacggcct
     2581 cgcgtcgtac atctggacac aggatgtcag caaagtgtta cgcctggcgc gtggcattga
     2641 agcaggcatg gtgttcgtca atacccagaa cgtccgcgac ctgcgccagc cgtttggcgg
     2701 cgtaaaagcc tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga
     2761 aatgaagaac gtctgcattt ccatgggcga ccatccaatt ccgaaatggg gagtctgata
     2821 tgggtaagtt agcgttagcc gccaagatca cgcacgtacc gtcgatgtat ctctctgaac
     2881 taccaggaaa aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa gagatcagca
     2941 agcgttgccg ggaaatgggc gtcgatacca ttatcgtttt cgacacccac tggctggtca
     3001 acagtgctta tcacatcaac tgtgcagacc attttgaagg cgtctacacc agtaacgaac
     3061 tgccgcattt tattcgtgac atgacctaca actacgaggg caacccggag ttggggcagc
     3121 taattgccga tgaagcctta aagctcggcg tgcgggcaaa agcgcacaat attcccagcc
     3181 tgaaactgga atacggcacg ctggtgccga tgcgctacat gaatgaagac aagcacttca
     3241 aagtggtctc catttcggct ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg
     3301 gcgaagcgat tctgaaagcg atcgaacagt acgacggcac cgtggcggtc cttgccagcg
     3361 gttcgttatc gcaccgcttt attgacgatc agcgtgcgga agaagggatg aacagctaca
     3421 cccgcgagtt cgaccgccag atggacgagc gcgtggtgaa gctgtggcgc gaaggccagt
     3481 tcaaagagtt ttgcaatatg ctgccggagt acgccgacta ctgctacggc gaaggcaata
     3541 tgcacgacac ggtgatgctg ctggggatgc tcggctggga taaatacgac ggcaaggtgg
     3601 agtttattac cgagctgttc ccaagctctg gcaccggtca ggttaacgct gttttcccgc
     3661 tgcccgcgta aggacgtttt atgccgcact ttatcgtgga atgtactgaa aatattcgtg
     3721 aagaagcccg cttacctgag ctgtttgcca gtgttaacac tgcactggcg gcaacgggga
     3781 tttttccgct tgggggaata cgtagccggg cgcactggat cgatacctgg caaatggctg
     3841 atggcaagca cgattacgcc tttgtgcata tgacgctaaa aatcggcagc ggacgcagtc
     3901 tggaaagccg tcaggaagta ggagaaatgc tgtttgatct gattaaaacg cacttcgcct
     3961 cactgatgga gagccgttac ctggcgcttt catttgagat tgcagagctg cacccgacgt
     4021 tgaatttcaa acagaacaac gtacacgcgt tgtttaaata aatttgcacc agcttccagg
     4081 ccggataagg cgcagcagca tccggcaatg ccacaggata tcgctatgtt cgacaaacac
     4141 acccacaccc tgatcgccca gcgtctggat caggcagaaa aacagcgcga acagatccgc
     4201 gcgatctcgc tggattaccc ggagatcacc atcgaagacg cctacgccgt acagcgcgaa
     4261 tgggttcgcc tgaagattgc cgaaggtcgc acgctgaaag gccacaaaat cggcctgacc
     4321 tcgaaagcga tgcaggccag ctcgcaaatc agcgaaccag attacggcgc actgctggac
     4381 gacatgttct tccacgatgg cagcgatatc ccgaccgatc gctttatcgt gccgcgcatt
     4441 gaagtggagc tggctttcgt gctggcaaaa ccgctgcgtg gaccaaactg cacgctgttc
     4501 gacgtttaca acgccacgga ctatgtgatc ccggcgctgg agctgatcga cgctcgctgc
     4561 cacaacatcg atccggaaac ccagcgcccg cgcaaagtgt tcgacaccat ttctgataac
     4621 gccgccaacg ccggagtgat cctcggcggt cgtccgatca aaccagacga actggatctg
     4681 cgttggatct ccgccctgat gtatcgcaat ggcgtgattg aagaaaccgg cgtcgccgct
     4741 ggtgtgctga atcatccggc aaacggcgtg gcctggctgg cgaacaaact cgcaccctat
     4801 gacgtacaac tggaagccgg gcaaatcatt ctcggtggtt cgttcacccg cccggttccg
     4861 gcgcgtaagg gcgacacctt ccacgtcgat tacggcaaca tgggttccat tagctgccgc
     4921 tttgtttaag gagagaacga tggaaaacag ttttaaagcg gcgctgaaag caggccgccc
     4981 gcagatcgga ttatggctgg ggctgagcag cagctacagc gcggagttac tggccggagc
     5041 aggattcgac tggttattga tcgacggtga gcacgcgccg aataacgtgc aaaccgtgct
     5101 cacccagcta caggcgattg cgccctaccc cagccagccg gtagtacgtc cgtcgtggaa
     5161 cgatccggtg caaatcaaac aactgctgga cgtcggcaca caaaccttgc tggtgccgat
     5221 ggtacaaaac gctgacgaag cccgtgaagc ggtacgcgcc acccgttatc cccccgccgg
     5281 tattcgcggt gtgggcagtg cgctggcccg agcctcgcgc tggaatcgca ttcctgatta
     5341 cctgcaaaaa gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat
     5401 gaagaactta ccgcagattc tggacgtgga aggggtcgac ggcgtgttta tcggcccggc
     5461 ggatctgagc gccgatatgg gttatgccgg taatcctcag cacccggaag tacaggccgc
     5521 gattgagcag gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc
     5581 caatgagcaa ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt
     5641 tgacaccacc ctgctcgccc gcgccgccga agcgctggca gcacggtttg gtgcgcaggc
     5701 tacagcgatt aagcccgacg tgtattaatg ccggatgcgc tgcgcttatc cggcctacac
     5761 tcgcaccaaa ccgtaggccg gataaggcgt ttacgccgca tccggcaaaa agccgtacca
     5821 aatcgcggac cggataaggc gtttgtgcgc atccgccata aaccttgtcg taccctacaa
     5881 aaatcccatt agaggaagta aaatgagcga cacctcacct gccataccgg agagtatcga
     5941 tccggcgaat cagcataaag cgctgactgc cggacaacag gcggttatta agaagctatt
     6001 tcgccgcctg atcgtctttc tgttcgtgct gtttatcttc tcgttccttg atcgcatcaa
     6061 catcggcttt gccggactca cgatgggacg cgacctcggt ctgagcgcca ccatgtttgg
     6121 cctcgctacc accttgttct acgccgctta cgtcatcttc ggcattccca gcaacattat
     6181 gctgagtatt gtcggtgcac ggcgctggat cgccaccatc atggtgcttt ggggcatcgc
     6241 ctctactgcc accatgtttg ccactggccc caccagctta tacgtactgc gtatactggt
     6301 tggcattacc gaagccggct ttctgcctgg cattctgctg tatttaacct tctggtttcc
     6361 ggcctatttc cgcgcccgtg ccaacgcctt gtttatggta gcaatgccgg taacgacagc
     6421 gttgggctcg atcgtttccg gctacatttt gtcgctggat ggcgtaatgg cattaaaagg
     6481 ctggcagtgg ctgtttttgc tggaaggctt cccgtcggta ttactcggcg tcatggtgtg
     6541 gttctggctt gatgactcac cggacaaagc taagtggctg acgaaagaag acaaaaaatg
     6601 cctgcaagag atgatggata acgatcgtct gacgctggtt cagccagagg gagccatcag
     6661 ccaccacgcc atgcaacaac gcagtatgtg gcgggagatc ttcactccgg tggtgatgat
     6721 gtataccctg gcgtatttct gcctgaccaa cacacttagt gcgatcagca tctggacacc
     6781 gcagatcctg caaagcttta atcagggcag cagtaatatc accatcggcc tgctggccgc
     6841 cgtaccgcag atttgtacca ttctcgggat gatctactgg agccgtcact cagatcgccg
     6901 ccaggaacga aggcatcaca ccgcccttcc ttatttgttc gctgccgctg gttggttact
     6961 ggcttcggca actgatcaca acatgatcca gatgctgggg atcattatgg cttcgaccgg
     7021 atcattcagc gcaatggcga ttttctggac aacaccggat cagtccatca gcctgcgggc
     7081 acgagcgatc ggtattgcgg tgatcaacgc cactggcaac attggttcag cgttaagtcc
     7141 gtttatgatc ggctggttga aagatctgac cggcagcttt aacagtggat tgtggtttgt
     7201 tgccacgctg ctggtgattg gtgcggggat tatctgggcg attccaatgc agtcctcccg
     7261 tccgcgagcg accccgtaag gaacgacgat gtgtgaccgt cagattgcca atattgatat
     7321 cagcaaagag tacgatgaaa gcctgggcac ggacgatgtg cattatcagt cgttcgcccg
     7381 catggcggcc ttttttggcc gccatatgct gccacatcgc cacgaacagt actttcagat
     7441 gcatttcctc aatagcggac agattgagct acagcttgac gatcatcgct actcggtgga
     7501 agcgcccctg tttgtcctga cgccgccgtc agtacctcat gcgtttatta cggagtctga
     7561 tgctgacggt catgtattga cggtacggga agatctgatc tggcccctgc tggaagttct
     7621 ttatccgggc actcgggaaa ccttcggcct gccggggatt tgcctgtcac tggcagataa
     7681 acccgacgaa ctggcggcgc tggaacacta ttggcaactg atagagcggg aatcggtaga
     7741 acaactgcct ggacgggaac acaccctgac gttactggca caggcagtgt tcaccctact
     7801 gctgcgtaac gcaaaactcg acgaccatgc cgccagcgga atgcgcggag aattaaaact
     7861 gttccagcgt tttcatatgc ttattgaaag ccattttcat cagcactgga cagtaccgga
     7921 ttacgctaac gaactgcata tcaccgaatc acgcctcacg gacatctgcc gccgctttgc
     7981 caaccgtccg ccaaaacggt tgattttcga caggcagcta cgagaagcca agcggctgct
     8041 gctgttttct gataacgccg tgaacaatat tgcctggcaa ctcggtttta aggatccggc
     8101 ttattttgcg cgctttttta atcgcttagt cggttgctcg cccagtgctt atcgtgccaa
     8161 aaaagtacct gtgacgtgaa attcccttag ccttaacggg aaaccaggca ccacctgcta
     8221 ttcccctttc tccgcctgga gcaaggggat ttagtaacgt taaaaatcgc taaaagcgac
     8281 ctcgatcaca aaaccgtacc cgacctgaaa agtaccagcc attgtcccaa aagtctcttc
     8341 ccgtaatcgc ccctgcaagc ttcaattgaa taacaaaaac aacacacaaa attaacaatc
     8401 gtattccgat taatactgta gaggtcgata tgaaaccaga agatttccgc gccagtaccc
     8461 aacgtccgtt caccggggaa gagtatctga aaagcctgca ggatggtcgc gagatctata
     8521 tctatggcga gcgagtgaaa gacgtcacca ctcatccggc atttcgtaat gcggcagcgt
     8581 ctgttgccca actgtacgac gcgctacaca aaccggagat gcaggactct ctgtgttgga
     8641 acaccgacac cggtagcggc ggctataccc ataaattctt ccgcgtggcg aaaagtgccg
     8701 acgacctgcg ccagcaacgc gacgccatcg ctgagtggtc acgcctgagc tatggctgga
     8761 tgggccgtac cccagactac aaagccgctt tcggttgcgc actgggcgcg aatccgggct
     8821 tttacggtca gttcgagcag aacgcccgta actggtacac ccgtattcag gaaactggcc
     8881 tctactttaa ccacgcgatt gttaacccac cgatcgatcg tcatttgccg accgataaag
     8941 tgaaagacgt ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg
     9001 cgaaagtggt tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg
     9061 cacaagtgat gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg
     9121 atggcgtgaa attaatctcc cgcgcctctt atgagatggt cgcgggtgct accggctcgc
     9181 cgtacgacta cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata
     9241 acgtgctgat cccatgggaa aacgtgctga tctaccgcga tttcgatcgc tgccgtcgct
     9301 ggacaatgga aggcggtttt gcccgtatgt atccgctgca agcctgtgtg cgcctggcag
     9361 tgaaattaga cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg
     9421 agttccgtgg tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg
     9481 cattgagtga ctcgatgtgt tcagaagcga cgccgtgggt caacggggct tatttaccgg
     9541 atcatgccgc actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa
     9601 acattatcga acgcaacgtt accagtggcc tgatctatct cccttccagt gcccgtgacc
     9661 tgaacaatcc gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg
     9721 atcacgtcca gcgcatcaag atcctcaaac tgatgtggga cgccattggc agcgaatttg
     9781 gtggtcgtca cgagctgtat gaaatcaact actccggtag ccaggatgag attcgcctgc
     9841 agtgtctgcg ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc
     9901 gctgcctgtc ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg
     9961 atatcaacat gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga
    10021 tgaacaacgc ctgcgctttc gtgacgcgat ggccagcctg tcggcagcgg taaatattat
    10081 caccaccgag ggcgacgccg gacaatgcgg gattacggca acggctgtct gctcggtcac
    10141 ggatacacca ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt
    10201 tcagggcaac ggtaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg
    10261 ccacttcgcg ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca
    10321 aaaaggtccg ctggcgcagc cggtgctgaa aggttcgctg gccagtcttg aaggagagat
    10381 ccgcgatgtg caggcgattg gcacacatct ggtgtatctg gtggagatta aaaacatcat
    10441 cctcagtgca gaaggtcacg gacttattta ctttaaacgc cgtttccatc cggtgatgct
    10501 ggaaatggaa gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggtatttt
    10561 tattgtgatc tcaatcacat atccacccga tgaataattc ttgcccaacc gctcaccgcc
    10621 attcctgacc gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgcg
    10681 cgtatctctg gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca
    10741 aacctagaac ggcttcggcc tatgattaac aaattactct atggattttg gtttgcagga
    10801 aggcagcaag tgagtgaatc cccgggagct tacaacagta agtgacaggg gtgaacgaac
    10861 gcagctgccg cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat
    10921 acgaaaaaac tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc
    10981 ctcgcggtag ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc
    11041 tctgtgtcgg tgtatctggt ggcttatcgc tactacagtc tgtacatcgc ccagaaggtg
    11101 atgaaactcg accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac
    11161 gttccgacca accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgctggt
    11221 ccgctggtgg gaccggttct cgccgcgcag atgggctacc tgcctggcac gctgtggctg
    11281 ctggcggggg tcgtgctggc cggtgcggtt caggacttta tggtgctgtt tatctcctct
    11341 cgccgtaacg gagcatctct cggtgagatg atcaaagaag agatgggacc agtaccgggg
    11401 actatcgcgc tgtttggctg tttcttaatc atgatcatca tcctcgccgt cctggcgctg
    11461 attgtggtta aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta
    11521 ccgattgcgc tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa
    11581 gtctctgtca ttggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct
    11641 cacgatccgt actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg
    11701 attggctatg cgtttgtttc cgcattgctg ccggtgtggc tgatcctcgc accgcgcgac
    11761 tatctggcaa ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggtg
    11821 ctgaacccgg aactgaaaat gcctgcaatg acccagtaca ttgacggtac tggcccgctg
    11881 tggaaaggcg ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc
    11941 ttccacgcgc tgatctcttc cggtacgacg ccgaaattgc tggctaacga aaccgacgcg
    12001 cgtttcattg gctacggcgc aatgctgatg gaatccttcg tggcgattat ggcgctggtt
    12061 gctgcgtcca tcatcgaacc gggtctttac ttcgcgatga ataccccgcc tgctggcctt
    12121 ggcatcacca tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg
    12181 cagctgaaag acgttaccgc acacgcggcg gcgaccgtca gctcctgggg cttcgtgatt
    12241 tcgccagagc agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt
    12301 gcaggtggcg cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg
    12361 atggctgaca tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg
    12421 accgcgctgg atgcgggtac tcgttctggc cgctttatgc tgcaagacct gctgggtaac
    12481 ttcatcccgt tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc
    12541 tgtgtgggtc tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt
    12601 aagagcctgt ggccgctgtt cggtatttcc aaccagatgc tggcagccgt agcgctggta
    12661 ctgggcaccg ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt
    12721 ccggctgtat ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc
    12781 aacccgcaga tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac
    12841 ggtactgacc tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac
    12901 accaacgcag gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt
    12961 ttcaaaacct ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aactccgtac
    13021 gttccaatcc cggaaggcgg cgtgaagatt tcttcgcacc actaaccgtg tttagccccg
    13081 cttcggcggg gctttgttct tatcagagtg aactatgttt ggtaacttag gacaggcaaa
    13141 aaaatatctc ggccaggcgg cgaagatgtt gattggcatt ccagactacg acaactacgt
    13201 cgagcatatg aagaccaacc atcccgacaa gccgtacatg agctatgaag aattcttccg
    13261 cgaacgccag aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct gttgttaatg
    13321 gagagagcat gaacccgatt gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa
    13381 ccaccctgct gcgccatatt cttaatgaac aacacggcta caagattgcc gtgattgaaa
    13441 acgaattcgg cgaagtctcc gttgatgatc aactgattgg cgaccgcgcc acgcagatca
    13501 aaacgctgac caacggctgc atctgctgtt cgcgctccaa cgagctggag gacgcgctac
    13561 tcgacctgct ggataatctc gacaagggca atattcagtt cgatcgtctg gtcattgaat
    13621 gcaccggcat ggccgatcct ggcccgatta ttcaaacctt tttctcccat gagattttat
    13681 gccagcgcta cctgctggac ggcgtgattg cgctggtgga tgcggtacat gccgatgagc
    13741 agatgaacca gttcaccatc gcccagtccc aggtgggcta cgccgaccgt attctgctga
    13801 ctaaaaccga cgtcgcaggc gaagcagaaa aactgcgtga acgtctggca cgcatcaacg
    13861 cccgtgcacc ggtctacacc gtcacccacg gcgacatcga cctgggtctg ctgttcaaca
    13921 ccaacggttt tatgctggaa gaaaacgtcg tcagcaccaa accgcgtttc cattttatcg
    13981 cggacaaaca aaacgatatt tcgtcgattg tggtggaact ggattacccg gtagatatca
    14041 gcgaagtttc ccgggtgatg gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt
    14101 acaaagggat gctgtggatt gacggcgaac ctaaccgcct gctgttccag ggcgtccagc
    14161 gcctctacag cgccgactgg gaccgcccgt ggggcgatga aaaaccgcat agcacgatgg
    14221 tgtttatcgg gattcagttg ccggaggaag agattcgggc ggcgtttgcg gggttgagga
    14281 agtaagttcg ggaaagatca actttttgag agttccaccg caacattatt acgagtggaa
    14341 ctctttagat atgtcagacc aaccaaagct tcttcaattg cgttttcatt ttttcatgtc
    14401 tcattttcag ttcagccaat ccccattctg aagaattaca aacatctttt gt
//
LOCUS       DAJOSY010000106        14337 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJOSY010000106 DAJOSY010000000
VERSION     DAJOSY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.106"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            97..1254
                     /gene="yehY"
                     /locus_tag="OHN27_003493"
     CDS             97..1254
                     /gene="yehY"
                     /locus_tag="OHN27_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCQ2287871.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            1247..2173
                     /gene="yehX"
                     /locus_tag="OHN27_003494"
     CDS             1247..2173
                     /gene="yehX"
                     /locus_tag="OHN27_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCQ2287872.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERADGEALAEEMTLRDALS
                     LFVARGCEVLPVVNIQGQPCGTLHFQDLLVEA"
     gene            2178..2909
                     /gene="yehW"
                     /locus_tag="OHN27_003495"
     CDS             2178..2909
                     /gene="yehW"
                     /locus_tag="OHN27_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCQ2287873.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2890..2997)
                     /locus_tag="OHN27_003496"
     CDS             complement(2890..2997)
                     /locus_tag="OHN27_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCQ2287874.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(3057..3788)
                     /gene="mlrA"
                     /locus_tag="OHN27_003497"
     CDS             complement(3057..3788)
                     /gene="mlrA"
                     /locus_tag="OHN27_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCQ2287875.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYSAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            4010..5695
                     /gene="btsS"
                     /locus_tag="OHN27_003498"
     CDS             4010..5695
                     /gene="btsS"
                     /locus_tag="OHN27_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCQ2287876.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            5692..6411
                     /gene="yehT"
                     /locus_tag="OHN27_003499"
     CDS             5692..6411
                     /gene="yehT"
                     /locus_tag="OHN27_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCQ2287877.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            6458..6928
                     /locus_tag="OHN27_003500"
     CDS             6458..6928
                     /locus_tag="OHN27_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCQ2287878.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(6968..7429)
                     /locus_tag="OHN27_003501"
     CDS             complement(6968..7429)
                     /locus_tag="OHN27_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCQ2287879.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(7554..9554)
                     /locus_tag="OHN27_003502"
     CDS             complement(7554..9554)
                     /locus_tag="OHN27_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCQ2287880.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHIVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEATFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(9551..10686)
                     /locus_tag="OHN27_003503"
                     /pseudo
     CDS             complement(9551..10686)
                     /locus_tag="OHN27_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            complement(10679..12958)
                     /locus_tag="OHN27_003504"
     CDS             complement(10679..12958)
                     /locus_tag="OHN27_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287881.1"
                     /translation="MSEPLIVGIRHQSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRLREAFMARWIGWAMQQNNGDVLVVCGGWHAPVLAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAVLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(12969..14057)
                     /locus_tag="OHN27_003505"
     CDS             complement(12969..14057)
                     /locus_tag="OHN27_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCQ2287882.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         3224 a         3664 c         3939 g         3510 t
ORIGIN      
        1 cagcaactga atgccagcat tgctgtggaa ggactggatg ccaaaaaagt ggctgccgac
       61 tacctgaaac aaaaagggtg gacgaagtaa tttcccgtga cttatttccg tattaatcct
      121 gttctggcgc tgctgctgtt gctgacggca atcgcagcgg cgctgccgtt tatcagttac
      181 gcgcctaatc gtttagtttc gggtgagggg cgtcatctct ggcagctgtg gccgcaaacg
      241 atctggatgc tggtgggcgt tggttgcgcc tggctgacgg cctgttttat tcccggtaaa
      301 aaaggcagca tttgtgcact cattctggcg caattcgtct tcgtattgct ggtgtggggc
      361 gcgggaaagg cggcgacaca actggcgcaa aatggcagtg cgctggcgcg taccagcctc
      421 ggcagtggtt tctggctggc tgcggcgctg gcattgctgg cctgtagcga tgccatccgc
      481 cgaatctcca cgcatccgct gtggcgctgg ttgttgcata tgcagattgc cattattccg
      541 ctgtggttgc tgtactccgg cacgcttaac gatctctcac taatgaaaga atacgccaac
      601 cgtcaggatg tgtttgacga cgcgctggca caacatctga cgttgctgtt tggtgcggtg
      661 ctgcctgcgt tagtgattgg tgtgccgttg ggcatctggt gctacttttc cactgctcgg
      721 cagggggcaa ttttttctct gctcaatgtc attcagaccg tgccttcggt ggcgctcttt
      781 ggcctgttga ttgcgccgct tgccgcgctg gttacggcct ttccgtggct ggggacgctc
      841 ggcatagcag gaaccggaat gacacccgca ctgattgcgc tggtgctcta tgccttgctg
      901 ccgctggtgc gcggcgtggt agtcggtttg aaccagatcc cgcgcgatgt gctggagagc
      961 gccagagcga tgggcatgag cggggcgcag cgattcctgc atgttcagtt accactggcg
     1021 ttaccggtat ttctgcgcag cctgcgggtg gtgatggtgc aaactgtagg tatggcggtg
     1081 attgcggcgt taatcggcgc aggcggtttt ggtgcgctgg ttttccaggg gctgctaagc
     1141 agcgccattg atttagtgtt gctgggggtg atcccggtaa ttgttctggc ggtgcttacc
     1201 gacgcgctgt tcgatttgct tatcgcactg ctgaaggtga aacgtaatga ttgaatttag
     1261 ccatgtcagc aaactgttcg gcgcacaaaa agccgttaac gatctcaatc tcaattttca
     1321 ggaagggagt ttttcggtgc tgattggtac atcaggctcc ggcaaatcca ccactctgaa
     1381 aatgattaac cgcctggtgg agcatgacag cggcgtgatc cgctttgccg gagaagaaat
     1441 tcgctcgctg ccagtactgg agttgcgccg ccggatgggc tatgccattc aatctattgg
     1501 cctgttcccc cactggagcg tggcgcaaaa catcgctacc gtgccgcaat tacaaaaatg
     1561 gtcgcgggcg cggattgacg atcgtatcga cgaattaatg gcgctgctgg ggctggagtc
     1621 aaatttgcgt gagcgttatc cgcatcagct ttccggtggt cagcagcaac gtgtgggagt
     1681 ggcgcgcgca ctggctgccg atccgcaagt cttactgatg gatgaacctt ttggcgcact
     1741 ggacccggta acgcgcggcg cgttgcaaca agagatgacg cgcattcacc gtttgctggg
     1801 gcgcactatt gtgctggtca ctcatgatat tgatgaggcg ctacggctgg cagaacatct
     1861 ggtattgatg gatcacggtg aagtggtgca gcagggcaat ccgctgacga tgctgactcg
     1921 tccggcgaat gattttgtcc gccagttttt tggacgtagt gaactgggtg tgcgcctgct
     1981 ttcgttacgt agtgtggcgg attacgtgcg tcgcgaagaa cgggcagatg gtgaggcact
     2041 ggcagaagag atgacgctac gcgatgcgct ctctctgttt gttgcgcggg gatgcgaggt
     2101 gctgccggtg gtgaacatcc agggccagcc ttgcggcacg ctgcattttc aggatctgct
     2161 ggtggaggcg taagcgtatg aagatgttgc gcgatccgct gttctggctc attgctttgt
     2221 ttgtggcact gattttctgg ctgccttaca gccagccgct gtttgctgcc ttgttcccac
     2281 aactgccacg acccgtttat cagcaagaaa gttttgcagc tctggcactg gctcatttct
     2341 ggctggtggg aatttcgagt ttgtttgcgg tgatcattgg cacgggtgcc ggaattgcag
     2401 tcactcgccc gtggggcgcg gaatttcgcc cactggtgga aactattgcc gccgttgggc
     2461 agacttttcc gcctgtcgca gtgctggcga ttgctgttcc ggtgataggc tttggtctgc
     2521 aaccagcgat tatcgccttg atcctttatg gtgtgctgcc cgtcctgcag gcgacacttg
     2581 ccgggctggg agcgattgat gccagcgtga cagaagttgc gaaaggtatg ggaatgagtc
     2641 gtggtcagcg actgcgtaag gtcgagctac cgctggcggc tccggtgatt ctggcgggcg
     2701 tgcgaacttc ggtgattatc aacattggta cggcgacgat cgcctcaacg gtaggggcca
     2761 gcacgctggg tacgcccatc atcatcgggc ttagcggatt taataccgcg tatgtgatcc
     2821 agggggcgtt actggtggca ctggcggcga tcatcgcaga ccgcctgttt gaaaggctgg
     2881 tgcaggcgct tagccagcac gcaaaataaa ggtataacct gcgagcatga cgccaccaat
     2941 tccgcctaac gccataaaca ggaacagggc aatgacccca attttagcta tgcgcataat
     3001 gcactcctta tgttaacgaa aggattgtac agtaaagcgc atttgttaac gaatcattaa
     3061 atgccgagtg ggaaaatatc atggccttgt tcctgccaac tggtgagttg ttgctgttgg
     3121 gcggaggttc gattttcacc gcaccacacc agcaatgtac ggccttcgaa tagttcaggg
     3181 cgtagttgat tgagcgagtg ggcgaggaca tcaatgcgcc atccttgttg actggcaatc
     3241 cagccctcca gccacagacg ggtggtatcc tgaatattcc agccaaccac cagcgcatct
     3301 ttaccctgtt ttttacgtgc cgaagccaga caaatggcga tgtagttgat cagtacgccg
     3361 tcgaggatcg ccagcagcgc ctggagagtc ggttgttggc actgaagccg tcggcgcaga
     3421 ggaataaaca gatgtgtggt gagtgtctgg gcggagtaat cctgaccgcg ctctttgatc
     3481 cacgttcgca ggctatgtag attgccgctt tgcaggtaag tcagtaatgt ttcttgctga
     3541 tcgcgccagc cgttctgcac atcaacattt tcattactga gcagcatttt aactttgctg
     3601 acctgcacgc cgttgtcgat ccagcgtttg atctcgcgga tccggtcaat atcggcatcg
     3661 ttgaacagcc gatgaccgcc gtctgtccgt tgcggtttca gcaatccgta acgcctctgc
     3721 cacgcgcgta acgtgacagg attaatatca caaagcaacg ccacttcacc aattgtgtaa
     3781 agcgccatcg tctcaccctt gctcgcgagg tcccggttta actttagacg cagttttgcg
     3841 aaccaggtag ttttgcccgt tttttgtgca tctatagggt gattttattt ttgccaggcg
     3901 attttgagtg atcgtactca cgaattctca tttttctgca agagttcaaa gaaagttaaa
     3961 cgcaggcaat gtatgttacg cgttttaaag ggaagtgtgg tttgcgggta tgtacgattt
     4021 taatctggtg ttgctgctgc ttcagcagat gtgcgttttt ttagtcattg cgtggttaat
     4081 gagtaaaacg ccattattca taccgttaat gcaggtcacg gttcgtctgc cgcataaatt
     4141 tctctgctac atcgtctttt ccatcttctg catcatgggc acctggtttg ggttgcacat
     4201 tgacgattct attgccaata cccgtgcgat aggcgcggta atgggcggct tactcggcgg
     4261 tccggtcgtc ggtgggctgg ttggcctgac cggcgggtta catcgatatt cgatgggggg
     4321 catgaccgcg ctaagttgca tgatctcgac catcgttgaa ggattgctcg gcggcctggt
     4381 acacagcatc ctgatccgtc gcgggcgcac tgataaagtc tttaacccca ttaccgccgg
     4441 tgccgtcacg ttcgtcgctg aaatggtgca aatgttgatc atcctggcga ttgcccgacc
     4501 ttatgaagat gcggtgcgtc tggtgagtaa tattgctgcg ccaatgatgg tcaccaatac
     4561 cgtcggcgcg gcgctgttta tgcgtatatt gctcgataaa cgcgcgatgt ttgaaaaata
     4621 cacttcagct ttttctgcca ctgcgctgaa agtggcggcc tcgacggaag gcattttgcg
     4681 ccaggggttt aacgaagtga acagcatgaa agtggcacag gtgctgtatc aggagctgga
     4741 tattggtgca gtcgcgatta ccgatcgaga gaaattgctg gcctttaccg gaattggtga
     4801 cgatcaccat ttacccggca aaccgatttc ttcgacttac accttaaaag cgattgaaac
     4861 cggtgaagtg gtctacgctg atggcaacga agtaccttat cgttgctctt tgcatccgca
     4921 atgcaaactg gggtcgacgc tggtaattcc gttgcgtggt gaaaatcagc gggtgatggg
     4981 caccatcaaa ttgtatgaag ccaaaaaccg tttattcagt tcaatcaacc gcacgctggg
     5041 cgaggggatt gcgcaactgc tttcggcgca gatcctcgcc gggcaatatg agcggcaaaa
     5101 agcgatgctc acccagtcag agatcaaact gcttcacgcc caggtgaacc cccatttttt
     5161 gtttaatgcg cttaacacca ttaaagcggt gatccgtcgc gacagcgaac aggccagcca
     5221 gctggtgcag tatctttcca cttttttccg caaaaactta aagcggcctt cggagtttgt
     5281 tactctcgcc gacgaaattg aacatgtgaa cgcttatctg caaattgaaa aggcgcgctt
     5341 ccagtcgcgg ttgcaggtca acattgctat tccgcaagaa ttatcccagc agcaattgcc
     5401 cgcgtttacc ctgcaaccga tagtggaaaa cgccattaaa catgggacat cacaactgct
     5461 ggatacaggg cgagtggcaa tcagcgcccg acgtgagggg caacatttga tgctggagat
     5521 cgaagacaat gccggtttgt atcaaccggt aaccaatgcc agtgggctgg ggatgaatct
     5581 ggtggataag cgtttacgtg aacggtttgg cgatgactat gggataagcg tcgcctgtga
     5641 gcctgatagt tacacccgaa taacgttacg actaccatgg agggacgagg catgattaaa
     5701 gtcttaattg tcgatgatga accgttagca cgggagaacc tgcgcgtatt tttgcaggag
     5761 cagagcgata ttgaaatcgt tggagagtgt tcaaacgccg tggaagggat cggcgcggtg
     5821 cataaactgc gcccggatgt gctgtttctc gatatccaga tgccgcgcat cagtggtctg
     5881 gaaatggtgg ggatgctcga tccggaacat cgtccgtata ttgtttttct caccgcgttt
     5941 gacgaatacg ctattaaagc ctttgaagaa catgcttttg attatctgct gaagccaatt
     6001 gatgaagcgc gactggagaa aacgctggcg cgattgcgtc aggagcgcag caagcaggat
     6061 gtttcgctgt taccggaaaa tcaacaggcg ctgaaattta tcccttgtac ggggcatagt
     6121 cggatttatt tgctgcaaat gaaagatgtg gcatttgtca gcagtcggat gagcggtgtc
     6181 tacgttacca gccacgaagg gaaagagggc tttaccgaat tgacattacg taccctggaa
     6241 agtcgtacac cactactgcg ctgccatcgt cagtatctgg ttaatctcgc gcatttacag
     6301 gagattcgtc tggaagataa tggccaggcc gagttgattt tgcgtaatgg cttaaccgtg
     6361 ccggtcagcc gccgttatct gaaaagctta aaagaggcga ttggcctgta aaagactgct
     6421 aaaatggctt tttgcctcat caacacctga aggcctcatg ctaagtaacg atattctgcg
     6481 cagcgtgcgc tacattttga aagccaataa taatgacctg gtgcgtattc tggcgctggg
     6541 taatgtcgaa gccaccgcgg aacagatcgc cgtctggcta cgtaaagaag acgaagaggg
     6601 ttttcagcgt tgtccggaca ttgttttgtc gtcattcctc aacggcctga tttatgaaaa
     6661 acgcggcaag gatgagtctg ctccggcact ggagccggaa cgtcgcatta ataacaacat
     6721 cgtgctgaaa aaattacgca tcgcgttttc gctgaaaacc gatgacattc tggcgatcct
     6781 caccgaacag cagttccgcg tttcgatgcc ggaaattaca gcgatgatgc gtgcaccgga
     6841 tcataaaaac ttccgcgaat gcggcgatca atttttacgt tattttctgc gtggactggc
     6901 agcgcgccag catgtgaaaa aaggctaaaa aattggcggt catgtttaga gcatgaccgc
     6961 caaccgatta tttcacttct ttaaaaccag cggctttcat caccagttcc atttgcgcca
     7021 tagtgatacc ttttttggca tcttcagcag aaacgttgat tcctgaaata ccctgcaggg
     7081 ctttaaaatc cactttttcc atatcgatag tcacgttttc ctgcgcgtag gtatcggtat
     7141 aggttaattt ttcttcaaca cccgcgatat ttttgtattt ggcgcttaac ggctcaagtg
     7201 tcttggcagc gtcttctttg gtggttgcac caatagaagc aaattgaatt ttggtttcag
     7261 aagattgctt aagcaccttg tcacctttgt agacataggt aatggcaatt tcagtgccgt
     7321 tcagattggc gctgaatttc ttcgattctt ctttgtcacc gcagccagca agagagaaaa
     7381 ccagaacaga tgcaacaacg agggaaaaca gcttattgaa agccttcatg taaaactcca
     7441 ttttatttaa tcaagaaact ggtgactctc accaggggct atatagaata tgcctaatac
     7501 cgtgacgtga gcagtccgga actggagtag aactcttagt aaaaagcact atttcatcct
     7561 tgttgctgaa gcatggggaa taattgttcg caaagtaaaa caccgttatt cattgcttct
     7621 acccgtgcct cgctttctgt attacgaaat tgtcccaaca catgtgccag ccgataaaaa
     7681 ccgaccgcgg agaggtcatt cgccagcaac tctgcctgac taatagcgct ctgttcctga
     7741 tagcgccagc cgttatggag cagttgaata agtaacgcct ggcagcgcat cagcaactga
     7801 tgagcggtag acggcacagg caaaacgctg gcagaaggta gcggtgccac aggcgcagtt
     7861 tctgcgtcca gcgcccaggc gcgggttttt gtcatcatca cccgtggttc cagtgtcaat
     7921 tgcccatcaa caaaactgac aaagccagaa accagactca cggggtcgtc tgtttgttgc
     7981 aaaagcgccg ccatgcgttc aacggcaaaa ggagaacagg cagatgccgg aagggataac
     8041 gtcaggagat tatcttcacc ttcgccgctg attacctgcg catccagcgt ctggcggctg
     8101 ctatcccaac cgagcgaaat acactcagcg accggcagaa taaataagtt atcgacctga
     8161 ttaagaggcc gtatgcaggc ggggggacgc tggcgtaaat attcccgcaa agccacaatg
     8221 cccggctggc gtaacggcgc gctcaacatt tgccaggcat caggcgacag cggcacaacg
     8281 ctgcttaagc ggttgcgggt agctaacagc agctcgccat cggcactgcg ttttgctgct
     8341 tgtgaaacaa tttgcccacc cgccagtgcg ccagcctgaa aactaaacag ccgacgcgta
     8401 gctgccggtg agttttcctg ttcacttcgc ggccaactgc gcgaaaggtg caaaatactg
     8461 ccggtgtcgg gatcggtaaa ccagatgcgt aaaccataat gctcaatatc ctgccagcaa
     8521 cgcataccta aagacaccag ccgcagatga tcaagctttg cttctccggc aatgccagag
     8581 ccaacgaccg tgcgccacgg cacaggagga acttcaccaa tactgtcgcg ccgggccatc
     8641 tcttgtgcgc aatttaatcg actgtttaat gccgcaagct gacgtaagca ttctccggca
     8701 tgatagtggc tggcgcgggc gtggaaggca tcaacgcttg cccgtagctg tcgtagtgat
     8761 tcactcaccc atcgccagtt gcagcgttcc gccgcctgct gcgcgcggct gaaagtggcc
     8821 tcgtagtgaa taagcggctg gctgatgccg ctcagccata atgcctggct tagttgttga
     8881 acatattgac gacacgtttt gccttcttcg ctggcaaacg gatcgtcaga tgatgtgacg
     8941 tgttcgctgc gcatctgcca gattaaatga gtcaattccg cttgctgcgc ttttgcctgg
     9001 acaaaggcct gtactgccag tacgatatgt tcgcaaagtg tgccttcaat acaatcacaa
     9061 cgggcgaaac gaatactgct gcgggaataa aaacgcacat cgctcatcgg taagcgggca
     9121 gagggaattt cacccggcgc acagaacaac tcaatggtga tgcctttagc gaccagcgcc
     9181 tgtgcgcgtt tgcgggtggc atcgggaagg gtagccagtt cttccagcca gattgccgga
     9241 tcccattcct cttcttttcc cgtaggctgg gcggtagcac aaagtcgttg ataacttaac
     9301 accagcatca cgcgatgacg gcacataccg ctggccccgc agctgcactg agcctctttc
     9361 agtgcctggc cgttcgccag ctgggtacgg acaccgtcac tgaaggtggc gattaaagcg
     9421 ccgttctcat ggctgatctc cgggacgttg ccattttcca gttccttaag actgcgctta
     9481 acaaaaccgg cattgcttaa cgccgtcagg gcctgcggtg tcagttctaa taattccgga
     9541 cgtagtgaat tcatgactga agattctccg caagccatga tgccagctcg cccggtgtca
     9601 tggcggctat ttgtgcgccg acatttacca gcgcctgggc cgtatcgtgg tcatagcaag
     9661 gtgttgctgt gctatcgagc gctgccagtc ccagcacttt gatgccgctc tggacacact
     9721 ttttcacctg atgcgtcagt aatgatgatg aacccccttc gtaaaaatcg ctcacgagga
     9781 taatgacgct tttcgctggt tgttcaataa gttgccgacc atactccacg gcactggcga
     9841 tattggtccc gccgcccaac tgtactttca ttaataactc taccggatcg gcaacgtctg
     9901 ccgtgagatc aacgacgctt gtgtcaaacg ccaccagatg ggtacgaatg ccgggtaact
     9961 gccacaaaca ggccgccatc accgcagagt ggatcaccga atcgaccatc gatccgcttt
    10021 gatcaaccag taagaccagt tgccattgtt cgctttggcg tttaatgcgg ctgttaaagc
    10081 gggggattcg atatacaact tgccgtgttg cgggtgccag tgttgcaggt tggcgcgcag
    10141 agtacttttg aaatcaaagt ttcgcgccag tggaataaat gaacggcgac ggcgatcgcg
    10201 gacaccagaa aaagcctggc gaacttcctt tgccagtcgc gccataattt cttcaacaac
    10261 ctggcgcact atctggcggg cggcagccag tacttcggga ttcatcagat gtttggtgtg
    10321 caaaacggcg cgtagcaggc tttcggaagg ctgcatacgt tccagcacgt cgagatttgt
    10381 caccacatct tcaatgccgt agcgcagtac ggcatcgctt tccagccgct caatcacctg
    10441 ttgcggaaac agcgtgtgaa tactgttgat ccactcaggg gtggtgagat ttgagccacc
    10501 taatccaccg gagcgttcac cacgctggag ccgtccagga tcgcgcccat acagccactc
    10561 cagcgcgtgg tctatctgcc gggcgttgtc atccagccca caaagcgtcg tttctgccgc
    10621 ttcgccaaga attaatcgcc agcgttgtag ctcacgggtg gtcagaagat cgttcagttc
    10681 agacatggaa aactccccag tgttgcagcg atgccagtgc ctgctgttcg agttgttgat
    10741 gatgtgcaat ggcttgcggt ggacaatgca acggcatttg cagtgccgaa acgggaagtt
    10801 gcgccagttg ataatgctca agcacctgat gcgccagcgt cccgcgttct cgtggcggta
    10861 gccaggccat tgccgcgcgt aaatcgggca gggcattggt aaaatcggca tcacttagtt
    10921 gatttaaatg actgctgaaa ccggcgataa atgccggctg acaggccagt tgatgacggg
    10981 ctaatgccag caaaccgtgc agcgcctcgc cggattgtgc cggggataac tgcgccagca
    11041 tcgtcagcgc cgcttcggca ctggcgttgg gatgttccag acgcattagt gcgccaagaa
    11101 ccgcgccgcg atccagcgcc ggggcatgaa tcgcctgact gcgtcgctcc agtaaggcta
    11161 ccgccgcaga aagcgaaacg ccggataaat taacgccgct atgtagatcg cgcagaatat
    11221 ggcaaagcgc ttgccagcta tgcaggtgag cgtgaaactc cttttcatcc ggtctgccgt
    11281 tagattcaca cagccacagc gtgcgatcga tagccgcgca taacgtcgtt tgtaatatct
    11341 gcgcgccttg cataccgcta atttcatcca gccgccataa ggcatatagc acttccagcg
    11401 cctggcccat ttcggcaaat tgattttcct gggcgattaa taatgtgagt tgctccagta
    11461 attgttgact gaaggacgcc agacccgcta acgccgcctg gctaagacat gtggtgatac
    11521 tgccgattcc acccgcgtcc agcatatcgg cttctaattt atggcgcgcg gcttcctgga
    11581 gtgtggcacc aaagcaggcg gcctcaatca atgccgcatg ctggctaagc gggcgggtta
    11641 atttccagtg ttcttcaccg ttgcctgcaa gtgtcagtgt gcttccctgc tggcgcacaa
    11701 tccctgggat ctccagtatt gccagccgat gtaacacctg actttgcgct agtccattgg
    11761 ggttaaagcg atttaaggta agctccgccg gaagggagat tgcggtacgt tccagttcgc
    11821 aggtgacatc ttttggcaga ggaggttgtg gcgtagaagg ggcaagttta ccgaatccgt
    11881 cacccgctaa tgtgtctatc aacgttagca gaatcggatc ggtgtcggga tgaataacgc
    11941 cgcgatagct ccacggcaac ggcgcattca gggcttcttt aatcagcgat cctgctatgg
    12001 catccagcca gtcagtgcgt aacggtagtg tatgaccgcg caactgtgcc agtgccattg
    12061 catgcagatg tgcggcagcc atatccgcgg tcgaagcagg caacttgtgc tggcgcaaac
    12121 gggtgagaac cgtttttagc agttgttctc ccgcctgctg taagccccac tgccagcacc
    12181 agttttgcca gaccggggca ggcattccgg aaaggtatcc tgccagcaca tcaaggcgct
    12241 tttcactgta gggggtgaga taacaacctg taacggcatc tgccagcgac ggcaattctg
    12301 gcgtgttaat ttcctgcggg cattcgcgcc acatttttgc cagtaccgga gcgtgccagc
    12361 caccgcagac aactaacacg tcgccattat tttgctgcat cgcccatcca atccagcggg
    12421 ccataaaggc ttcgcgcaga cgattgagag catctccggg ggagtcgccc cgcagttggg
    12481 caaaatagtg cgccagtgca gagggtaatg cagtttgctg gctttcatct tcgaacaagt
    12541 gatcccacag ggtgtcgctg ttatccatgc gggtggcacg cagcagcaag gcctggcttt
    12601 cctcttgcgt atcaggcgaa tcgtccactt cttcactctg cgcccagcaa ggcaaatcga
    12661 tgaagtaagt ttgtgcctga atacgacgtg cggcttgtag cgcctgccac tccggcgaaa
    12721 attcagcaaa tggcgtccag gcaccacgcc cgggggctgc accgtcctga tactggcaat
    12781 aactgtaaat agctaccgga agctggtggg ctaaaaacag ttcgtctacc cggtcattaa
    12841 aatcagccgg gccttcaatc aatacgtatc gtggccgctg gctttcgatt aacgatttca
    12901 ccagccgggc gcaggccgga ctttgatgcc ggatgccgac aattaacggc tcgctcatgc
    12961 atctttcctc acggcaggcg gtggcgggct tgataataag cctgccagtg agcttcttta
    13021 tgtgtggcga ctcgttgttc gaagtaacgg cgcagacgag cgcgatcttc ttcgttatct
    13081 ttgacgatgg ttccggcaat gcagtccacc aggtcggctg gctcacctgc gcgattcgct
    13141 aaaaaccagg cgcggacgcc tacggcgtgg gccacgttca cggcttcagc ggtggacata
    13201 atcgctgtta gtgtatccat tgacgttttc ttttcgccgt tggcacgcag atcgcggaag
    13261 gtgcggacca gtaactccag taccgcgtca ggcactttat gcggaatacc gctatgggcc
    13321 agcaaacgcg ccgaagcact ggcgacaagc tccagctcct gggcgaaatc cataatcgga
    13381 aagacagttt caaaatcgaa gcgacgcttt agcgcagcac tcatttcatt gacgccgcga
    13441 tcgcgggtat tggcagtggc aataatatta aagccttctc gcgcatagag ctgactggct
    13501 tcaccagtga gttccggcac cgtcatcacc ctgtcagaaa gcatacctaa cagacagtct
    13561 tgcacttcca gcggtgtacg cgttatctct tcaaaacgga cgattttgcc gtcgcgcatt
    13621 ccctggtaaa gcggggcagg gaccagcgct tccgttgatg gaccatgatt gataagcagg
    13681 gcgtagttcc agccgtactt gatttgatct tcggtagtag atgcgccgcc ctggatggtt
    13741 aacccggcat cgccgctaat agcggttgcc agtaattcag acaggagaga ttttgcggtg
    13801 ccaggttccc ccaccaacat caaaccgcgt ccggtggcaa gagtgactaa catacgttcg
    13861 acagcggagg gactaatcac cactttacgg ctaatatttt gcgcgctatc gccaaggata
    13921 aaagcacgtg ccgcaggcaa acttaactgc caaccgggcg ggcaaggtgc gttatcattt
    13981 tgttttaatt ttgccagttc atcggcgtat aacacagcag caggcggacg ctgcagatga
    14041 ttattctgcg gtgacatata ttcatccctg aaatgtatta atgaaataaa cgatatttta
    14101 cgcgcaataa atgtttggcg cattagttgt ttgagcggaa aatatctgga aagcagatca
    14161 cagtaaataa aaaaaactgc tggtgaggca ggttctttta tttactattt caggcccaac
    14221 accgcagcct gtgcaacatc gtgttccaac gaacgaatta taaccacggc tggaccccgt
    14281 tgcaattaag ataatgagat ttataatcca ggccattggg acgttgcgcc ggatcgg
//
LOCUS       DAJOSY010000107        13816 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJOSY010000107 DAJOSY010000000
VERSION     DAJOSY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.107"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..869)
                     /gene="yhaJ"
                     /locus_tag="OHN27_003506"
     CDS             complement(<1..869)
                     /gene="yhaJ"
                     /locus_tag="OHN27_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCQ2287883.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTSAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLR"
     gene            974..1675
                     /locus_tag="OHN27_003507"
     CDS             974..1675
                     /locus_tag="OHN27_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCQ2287884.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1698..1862
                     /locus_tag="OHN27_003508"
     CDS             1698..1862
                     /locus_tag="OHN27_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287885.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1996..3306)
                     /locus_tag="OHN27_003509"
     CDS             complement(1996..3306)
                     /locus_tag="OHN27_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCQ2287886.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3334..4665)
                     /locus_tag="OHN27_003510"
     CDS             complement(3334..4665)
                     /locus_tag="OHN27_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCQ2287887.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4941..6305)
                     /gene="tdcG"
                     /locus_tag="OHN27_003511"
     CDS             complement(4941..6305)
                     /gene="tdcG"
                     /locus_tag="OHN27_003511"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCQ2287888.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6377..6766)
                     /locus_tag="OHN27_003512"
     CDS             complement(6377..6766)
                     /locus_tag="OHN27_003512"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCQ2287889.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6780..9074)
                     /gene="tdcE"
                     /locus_tag="OHN27_003513"
     CDS             complement(6780..9074)
                     /gene="tdcE"
                     /locus_tag="OHN27_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCQ2287890.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLNIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9108..10316)
                     /gene="tdcD"
                     /locus_tag="OHN27_003514"
     CDS             complement(9108..10316)
                     /gene="tdcD"
                     /locus_tag="OHN27_003514"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCQ2287891.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10342..11673)
                     /gene="tdcC"
                     /locus_tag="OHN27_003515"
     CDS             complement(10342..11673)
                     /gene="tdcC"
                     /locus_tag="OHN27_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCQ2287892.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11695..12684)
                     /gene="tdcB"
                     /locus_tag="OHN27_003516"
     CDS             complement(11695..12684)
                     /gene="tdcB"
                     /locus_tag="OHN27_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCQ2287893.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12783..13721)
                     /gene="tdcA"
                     /locus_tag="OHN27_003517"
     CDS             complement(12783..13721)
                     /gene="tdcA"
                     /locus_tag="OHN27_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCQ2287894.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3584 a         3743 c         3246 g         3243 t
ORIGIN      
        1 tcccgcagac accaggattt cgcttccccc atactgtcac gacgccaggc cataataata
       61 tcgatctcgc tggtcgattc cgggctgacg acacgcaacc gcccttccgc aatatctttt
      121 tcgaccatag gatacggcat cgtcgccacg ccaagccccg ccagtaatgc ctgacgttta
      181 tcttcaatcg tgctcaccgt taagcgcggc tgtttgtcca gcagctgtac ggtcaacacc
      241 gggcgctcac gagcggtatc cgccaccgca attccacgat atttcacacg cgtcacttca
      301 gataacggtt ccggctcctg atgaatcggg tgatcaggcg cggcaacgta gacgttcatt
      361 aacgtataga gcttgcgcga gttgatctcc gacgaggaac gaaaatgcat atccggcgcg
      421 ataacaatat ccgcccgccc ctgctccagc cgttcccacg cccccgccag cacttctgtg
      481 atgattgcca gttgggtatt ggcttttgcc gccagtttgt cgattaacgg gaaaaaggca
      541 gatgtcggta ccagcgcttc ggtcacaatg gtgagatgcg tttcccaacc gcgcgcgaga
      601 gcttccgcat cagtagtcag tttatctgcg gcttccagca aaacgcgccc tcgctccaac
      661 aacatcctcc cgacattggt gaatttggta cgatggcccg agcggtcaaa cagcaccaca
      721 tccagctctt cttccagttt ttgcatggtg tagctaagtg cggaaggcac gcgtcccagc
      781 tcatccgccg ccgccgcaaa actgccccgg cgatcgatcg catccataac ccgtagtgct
      841 tccagcgtta atgccctttc tttggccatt tcgttctcat tcaggaaatt tgaacatacc
      901 gggcagaata tctggctaac aatgtagcgt ccagcccctt accataaaag gaagtaaaga
      961 gaggtcaaga attatgatta ctacccgaac tgccaggcag tgtggacaag cagactacgg
     1021 atggttgcag gcccggtata ctttttcctt tggacactac ttcgacccga aattgttagg
     1081 ctatgcctcc ctgcgtgtgc ttaaccagga agtgctggcc ccaggtgccg cctttcagcc
     1141 gcgaacctat cccaaagtcg atattttaaa tgtgattctg gatggagaag cagagtatcg
     1201 cgatagcgaa ggcaatcatg ttcaggccag cgccggagag gcgttgctgc tctctaccca
     1261 gccgggtgtc agctatagcg aacacaatct cagcaaagac aaaccgttaa cgcgaatgca
     1321 gctttggctg gacgcctgcc cgcagcgaga gaatccgctg attcaaaagc tggcgcttaa
     1381 tatgggcaag cagcaattaa tcgcctcgcc agagggtgcg atgggaagcc tgcaattacg
     1441 ccagcaagtg tggctgcacc atatcgtgct cgacaaaggc gaaagtgcga atttccagtt
     1501 gcatgggcca cgcgcgtatt tgcaatcgat tcacgggaaa tttcatgcgc ttacgcatca
     1561 tgaagagaaa gcggcgctga cctgcggtga tggggcgttt attcgtgacg aggctaacat
     1621 tacgctggtt gccgattccc cactgcgcgc tttgctgata gatttgcctg tctagttgtt
     1681 tttacaggga gatgatgatg agtaaaaaat cggccaaaaa gcgccagcca gtgaagcccg
     1741 tggtggcgaa agaacctgct cgcaccgcca aaaattttgg ctatgaagag atgttgagcg
     1801 agctggaagc catcgtcgcg gatgctgaaa cgcgtttagc cgaggatgaa gctaccgcgt
     1861 aaagacaggt tgccggatgc ggcgaaaacg ccttatccgg cctacaaaat ccaacaacat
     1921 taacgaatta aacaacccgt aggccggata agatgcgcca gcatcgcatc cggcacgata
     1981 cccaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct gccgatccgt
     2041 ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct caacatcatg
     2101 cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta acaccgcttt
     2161 ccacgcagcc gaagcactgg tcgaaacctt catcgcacag ctgttcgacg ctccatcgca
     2221 aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatag tttcataacg
     2281 cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca
     2341 aagtgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat gcgaaagcat
     2401 cagcgcacgc gccagccgtt catcatccgc tccgaagtgt tctgctacca ccaccacagg
     2461 cattgttgcg gtgatcccct ggttacccga gccagagtta ctcattgccg gaagcgtagc
     2521 gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg aagagagatc
     2581 tttcgccagc aaaccgcgcg cgcactgttt ttccagcgtc gcgccaatat gcagccccca
     2641 cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcagaat cgagaataaa
     2701 gcggatcgcc gcaaacggga cttcattgac gaacttcagg atctcagcca gcgtcgttct
     2761 ggaaagcacc gtcagcggag actcttgctc gccctctgcc acacacgcct gctgggtaaa
     2821 caccacacca tcgtgcgtct caatatgcac aatgttggta tgcccgccga cgatggtgac
     2881 acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt cattgcaagg
     2941 ttcctggatc ttaacggaga ctttccccgc cgccagcagt gctttggcat cggcaattgc
     3001 ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac ctaacgcccc
     3061 cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga cgcccagacc
     3121 gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac cttccagttc
     3181 tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg
     3241 ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta aagtcgaatc
     3301 aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga acggagaaac
     3361 acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt atttgtgcaa
     3421 tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg
     3481 gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc tccaggccag
     3541 caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct tctcggcagg
     3601 catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa agcctaaata
     3661 gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga ccactttcac
     3721 ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg aaatattctg
     3781 ctcgtaggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa
     3841 aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg ctttatgccg
     3901 cgccacttca atggattttt cccgcgagcg ataagagatc accatcgggc ttaacgtctg
     3961 aataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt tcaccagcag
     4021 ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca tcgacacgcc
     4081 cagcgccgcg accaccagca gcttggtcag caccatgccg gtcgaaattt tgaataacaa
     4141 tttctcgccg cgtgaggaga tcgccaccag aatgcaaatc agtaccagac cataaaaggg
     4201 actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc aggtaggaag cactatcgtt
     4261 ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat agagcgcacc
     4321 taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt aatctttaca
     4381 ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca ttgccgggta
     4441 accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct gcaccgggag
     4501 aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc cggtgtcagt
     4561 actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac gacccgccgg
     4621 ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt ctcgctcaaa
     4681 attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg gtctacgttt
     4741 ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa aatcttcaca
     4801 atcaagactt tgaaataacg gagatggata agaattttct actaaattaa tcgcaagaga
     4861 atattcattc tctatttgcg gcagatcaca aaaaaaaagg tgcacatttg tgcacccaag
     4921 gatgaaagct gacagcaatg tcagccgcag accactttaa tggccagtcc tccgcgtgat
     4981 gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc gatcacttta
     5041 tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct
     5101 ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc ggcaaccgga
     5161 tcgcaggtca gcccaaggtt atgctccatc gcgatttccg ccgcattgca tacctgcgcc
     5221 ggactgccgc ccagtagttc agttaaccct gccgccgcca ttgaacaggc cacgccaatc
     5281 tccccctgac agccgacttc cgcgccagag atggaggcgt tcattttata cagcgcgcca
     5341 atagcccccg cggccagaaa atagcgggca attgaccgct cgtttaccgg acgacggaac
     5401 ttatcgtaat aagccagtac tgccggaata atgccgcacg caccgttagt cggtgccgtt
     5461 accacgcgcc cgccagctgc gttttcttca ctaaccgcca gcgcgtacat gttgatccag
     5521 tcgatgacat tcatcggatc gttagagatg ttatcgctgg aaaccagctg acgacgcagc
     5581 gctacggcac ggcgcggcac attgagcgga ccaggcagca cgccttcagt gttcatccca
     5641 cgttcaatac cgtcatgcat cacttgccag atacgggcaa aaccggcgtc aatttccgct
     5701 ttgctgcgca gcgctagctc gttgtgcatc atcagaccag atatagacag gccgttgtaa
     5761 tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacgga cgtttcgaca
     5821 tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac agagtaatag
     5881 gttttactta atagctcttc ctgtcccttc caggcagtga tccgcattcc gttctcatgg
     5941 cgaggcaaca tttcgggatg gaagataatg ttctttgcta caggaaaatc aacaatatgc
     6001 gcaccagatg ccactggcag ccgcccgctg cgcgttacta actctataaa tgcagggatc
     6061 tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgatgat ggcgacatcc
     6121 gtggcatggc ctttgcccgt cagtgacaac gacccgtaca gatcgaccac aatatggctc
     6181 gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc ggcattcatt
     6241 ggccccacgg tatgcgaact ggagggacca atcccaattt tgaaaatatc gaatgcacta
     6301 atcatatcca caccctcgga ttgccgttca gtgaagtgga gcggaacgac cttacgaccg
     6361 tcccgctcac gaggctttac gcactacgta ctgcgatggc ttcaatttcc agcttcacat
     6421 ctttcggcaa acgcgcgacc tgcacacagc tccgggtcgg ataggtcgcc tgatgctcat
     6481 cgaagaactg cttatagact tcgttgatgg tggcaaaatc attcagatcg gtgataaaca
     6541 cggtcatctt gatgatatcg cccacgctca gcccggcagc aaccacgatc gctttgacgt
     6601 tttcgaggct taaacgcgcc tgatcttgca catcagccgg gatctcaccg gtctgtgggc
     6661 aaaccggtat ttgcccggag gtgaagacca tgctgcctaa atcaacgccc tgaacataag
     6721 ggccgattgc gcctggggca cgttgcgttt cgataatctt tttcatacat cctccggcgt
     6781 cagagcgcct gggtaaaggt acgtgaaata acatcctgtt gctgttcacg ggtcagtgcg
     6841 ttgaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt ttccgggtgc
     6901 tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg ttgaccgcct
     6961 tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt ggttttacgt
     7021 actggatctt ctttgcccaa cgccgcagga acgattgaga aggtgtacga gatcccatct
     7081 ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc acctttgcgg
     7141 tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg acgaccgtcc
     7201 ggcgtattac cggttttctg gccgtacacc acgttagaag tgatagtcag aatcgactgg
     7261 gtagggacgg cgttgcgata ggttggcagc gctttaattt tcttcataaa gcgttcaacc
     7321 aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg atattcaccg
     7381 tcgatttcaa agtccaccgc caggccgttt tcgtcacgga ttggtttcac gcgggcatat
     7441 ttgatggcag acagggagtc cgtcgccacc gacaggcccg cgatgccgca tgccatagtg
     7501 cgatagacat cacgatcgtg cagcgccatc agcgaagctt cgtagctgta cttgtcgtgc
     7561 atgtagtgaa tgatattcag cgcgctgatg tactgcaccg ccagccagtc catgaagtga
     7621 tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt ccatcagcgg tgctgttttc
     7681 ggcccgacct gaatattcag cttctcgtcc accccgccgt taattgcgta gagcagcgtt
     7741 ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct taccaatcac cattgggctg
     7801 acgcagcagg caatcgcgta atcgtcgctg ttgaagtcag tacgcatcag atcgtcattt
     7861 tcatactgca aggaagaggt gacgatcgac acctgcgcgg catatttttt gaaggcaatc
     7921 ggtaattctt ccgaccaaag aatggtcagg ttaggctccg gtgccggccc catagtgtgc
     7981 agggtgtgca aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc cagccccatc
     8041 ccgccgatca cttccgtcgc ccagattggg tcgccggaga acagcgaatc aaattccggc
     8101 gtacgcaaga agcgcaccat acggatcttc ataatgaagt gatcgatcag ttcctgtgcc
     8161 tgctgctcat tgagtacgcc tgctttaaag tcgcgttcaa tgtagatatc gaggaacgat
     8221 gccgtgcgcc ccagcgacat cgcgccgccg ttctgtgatt tcactgccgc cagataagcg
     8281 aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg ggcgagagat atcaaagcca
     8341 tatttcgccg ccatttcctg aatctgcaac agcgcatgac gatgctctgc cagctcctca
     8401 cgcagacgga tggtggcttc cagatcctcg cctttttcca gacgagactg gagatcggca
     8461 aattgcagtt cgcgttcacg caccagataa cgaatgccat acagcgctac gcggcgatag
     8521 tcaccgataa tgcgcccacg tccataacca tctggtaaac cggtcagtac gccagattta
     8581 cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg ggttttacgc
     8641 agatcggtaa acagatattc aaattcactg tccatttctc ggccataggc gtggaatgaa
     8701 cttttaatca tattaatgcc accgaacggg tgtagcgcac gtttcaacgg cgcatccgtt
     8761 tgcaggccaa caattttttc cagcggctgg ttaatatatc ccgcatcatg agcggtaatt
     8821 gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg ttgcattttc gatacggatg
     8881 ccttccatta ctttttccca caattccgtg gtggcaggcg tcgcttcggc gaggaaagat
     8941 tcatcgcctt catacggtgt atagttatgt tgaataaaat cgcggacatt aatttcgttt
     9001 ttccagtccg tacctttaaa gccaagccat gcgtcggcgt acagcttatc gctggtatca
     9061 atatctacct tcatgaaaaa taatctctct acaatacttc aactaaatta tgcaaattct
     9121 gcgggcgcgt taactttgcc taaatgaatg gcatccaaag caatcatttt ttcttcgtta
     9181 gtcggaataa cggcacaaat gacacgcgca ttttcactgg aaacaattcg ctctccaaaa
     9241 gagttagatc gattattcat ttctgtatca atcactacgc ctaatacagc caaatgttcc
     9301 atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa tataattcca
     9361 tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac gggcaattcg gtgaacaaag
     9421 gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc cagaacacgt
     9481 aaatccgaag aaagaccgga aatacctaat aatcccgact ctttattcac tacgcgttcc
     9541 aggtcaccca ggctctggtt ggtttggctg gcgacccagg acatcgcacc aaagtcgaca
     9601 tcgccactgc gggtacccat catcaagcct tccagcggcg tcattcccat tgaagtatca
     9661 acactctgac cgttgcgaac cgcgcagatt gacgcgccat tgccaagatg cgccacaacc
     9721 aggccggagt catcttccgc cagattcagc agcgaatgtg cgcgctggga aacatagcgg
     9781 tgcgacgtgc cgtggaaacc ataacggcgt acacctaact cttcataata tttccacggc
     9841 aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc aaataccgcc
     9901 acctgagtta cgcccggaaa taattgctgc gccgattcaa taccacttaa attggcgtaa
     9961 ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc atcggtaata
    10021 atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat taaggccacg
    10081 ctgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc accttcgtag
    10141 ctgtggtgag ccagcggtgc tggctctccc ccatttacgg ataagaatgc attttccgag
    10201 ttaataccgt cggcaatacc tgacattaat acttcacagt cgctggcatc gagtacggaa
    10261 aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc attcatctct
    10321 tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga tggtcagcag
    10381 accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca gagacggcgc
    10441 tttacggatg gcatacatcg gcaacaggca cagcagggat gcgataatcg gtgcgcccat
    10501 ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccagg tggagcccat
    10561 gatgaagatc atgctgagag tattcagttt acccagcgac actttggttt tgtcaccttt
    10621 ataaccgaac ttcagaatca gaccattcaa gccttccagc gtccccagat agtgaccgaa
    10681 gaaagatttg aagatagcca cgagtgcgat gatggaagcc gcatattcca gtgtaatcgc
    10741 gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta gccagataag aaagcactgg
    10801 aatattctgc gctttggctt ccgccatgtt ggccggagac agagtaaaca ggcagctaaa
    10861 ggcaaagaac atcaccactg caaccatcag catgctggca cgagaaatga tttgggaaca
    10921 tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt tagaaaccac
    10981 gaaggaagag acgattggcg agaagttaaa ggagaaaacc atgatggaaa tccccagcca
    11041 gacagtgatc aggataccgt catgaccggt taacgacagc gaaccgaggt caacctggtc
    11101 gataactgca gagttccagt aagggatcag cgacaaagaa atcagcacca ggctggcgat
    11161 aaacggccat accaggtagc tcatcacttt aaccatcaga tccttaccaa accagatgac
    11221 gaaagccatc agcagcaaca ggaacagcgc cacaaagccg cgattcagcg gtgcaaagcc
    11281 gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat aaatccacag
    11341 cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag ttttaccaaa
    11401 atgctcttcc accgtttccg taatgttgcc ggaagggtta gagccagaaa gacacagacg
    11461 cgccagcgcc cggtggcaat aaaacgcgat ggggtatgcc aataccaaca tcagaagaat
    11521 cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg cgccgattgc
    11581 cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg attgttttgt
    11641 ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa ttaattaagc
    11701 gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc cggaaataat
    11761 actgacggtt tttctgtttt gaatatattg gtctaattta ccgcttaata atgcagcaca
    11821 tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg caatcatact
    11881 gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac gaacgatttc
    11941 gtaagttaaa ttacccgggc gggagacatc acaaccatcc gccagggtgc cggtagttcg
    12001 gtgcgtggtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt tttcagactg
    12061 tacgccaata acacgaatgg tcgggttaat agatttaatt gccaccgcaa taccagcaat
    12121 taaaccgcca ccaccaattg gcacaatcac gttatcgaca tcatagagat cttccataat
    12181 ttccagacca atcgttccct ggccagcaat cactttcgga tcatcgtaag gtgggataaa
    12241 aatacggcct tccatttcga caatttcgct cactttagcg atagtgtcgt tgaagttatc
    12301 accatgcaga acgacttctg cggagtagtc gcacgttgcc gctactttgg attttggcgc
    12361 accttttggc atcaccactt taccgtcgat acccagcatc gcgcaggaga gggaaacccc
    12421 ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg cgtttttccg catcggtcag
    12481 tgaacttaat ttattaaatg cgccacgaat tttaaatgaa cccgtacgct gcatattttc
    12541 aaacttcagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc gaggcatgcc
    12601 tgttttataa attcgcccag ccagtcgttg tttcgcttca ataatgtcat caatagcaac
    12661 cggcagatcg tatgtaatat gcattataaa acctcttcgc ctgaattaaa tatgtaggta
    12721 accgtaaccg acacctgcaa gacagacagg tggattattt atgtttaaat aaaaacaaat
    12781 aactaaccaa cttctattaa ttgccttcgt ctacacccat tataagatga atactctttg
    12841 gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga ccataccgcg
    12901 gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg attagaacca
    12961 aaaggtgacg tcatatcaca aggaattaca gttaagaaat cagcattgag aacaagatta
    13021 taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc atttctttgt
    13081 aacgtagtaa gcagttcgct gtagtacccc atattagttt gtggcaacac ccactgttcg
    13141 ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact ggctaccagc
    13201 acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat ttctgcactt
    13261 aacgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa cgaagacagt
    13321 tgcgcttcat acatagaaac ctgcgctttc gggaacacct ctttgaactt gttgatcatc
    13381 cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc caccaccgcc
    13441 tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt aatggattcg
    13501 gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt tttccgcacc
    13561 actaattcca caccaaaata atcttcaata tcgttaatga ttttactgac ggccggttga
    13621 gttaacccta attcttttgc agccgagccg atagaaccac ttctaatgac ttcctgaaag
    13681 actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta cgttaattac
    13741 ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag gatatgtgcg accactcaca
    13801 aattaacttt caatac
//
LOCUS       DAJOSY010000108        13674 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJOSY010000108 DAJOSY010000000
VERSION     DAJOSY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.108"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            140..1537
                     /gene="ulaA"
                     /locus_tag="OHN27_003518"
     CDS             140..1537
                     /gene="ulaA"
                     /locus_tag="OHN27_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCQ2287895.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1553..1858
                     /gene="ulaB"
                     /locus_tag="OHN27_003519"
     CDS             1553..1858
                     /gene="ulaB"
                     /locus_tag="OHN27_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCQ2287896.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1868..2332
                     /gene="ulaC"
                     /locus_tag="OHN27_003520"
     CDS             1868..2332
                     /gene="ulaC"
                     /locus_tag="OHN27_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCQ2287897.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2346..2996
                     /gene="ulaD"
                     /locus_tag="OHN27_003521"
     CDS             2346..2996
                     /gene="ulaD"
                     /locus_tag="OHN27_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCQ2287898.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            3006..3860
                     /gene="ulaE"
                     /locus_tag="OHN27_003522"
                     /pseudo
     CDS             3006..3860
                     /gene="ulaE"
                     /locus_tag="OHN27_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
     gene            3860..4546
                     /gene="ulaF"
                     /locus_tag="OHN27_003523"
     CDS             3860..4546
                     /gene="ulaF"
                     /locus_tag="OHN27_003523"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCQ2287899.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4675..4950)
                     /locus_tag="OHN27_003524"
     CDS             complement(4675..4950)
                     /locus_tag="OHN27_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2287900.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5278..5673
                     /gene="rpsF"
                     /locus_tag="OHN27_003525"
     CDS             5278..5673
                     /gene="rpsF"
                     /locus_tag="OHN27_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCQ2287901.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDADAGDSEE"
     gene            5680..5994
                     /gene="priB"
                     /locus_tag="OHN27_003526"
     CDS             5680..5994
                     /gene="priB"
                     /locus_tag="OHN27_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCQ2287902.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5999..6226
                     /gene="rpsR"
                     /locus_tag="OHN27_003527"
     CDS             5999..6226
                     /gene="rpsR"
                     /locus_tag="OHN27_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCQ2287903.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6268..6717
                     /gene="rplI"
                     /locus_tag="OHN27_003528"
     CDS             6268..6717
                     /gene="rplI"
                     /locus_tag="OHN27_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCQ2287904.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6829..7800)
                     /locus_tag="OHN27_003529"
     CDS             complement(6829..7800)
                     /locus_tag="OHN27_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCQ2287905.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSQENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(7853..9154)
                     /locus_tag="OHN27_003530"
     CDS             complement(7853..9154)
                     /locus_tag="OHN27_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCQ2287906.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            complement(9197..9673)
                     /locus_tag="OHN27_003531"
     CDS             complement(9197..9673)
                     /locus_tag="OHN27_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCQ2287907.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            complement(9694..11775)
                     /locus_tag="OHN27_003532"
     CDS             complement(9694..11775)
                     /locus_tag="OHN27_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCQ2287908.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            complement(11772..13313)
                     /locus_tag="OHN27_003533"
     CDS             complement(11772..13313)
                     /locus_tag="OHN27_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCQ2287909.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDELVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDITPDGLHIAQSGQIPKLVTKVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(13323..>13674)
                     /locus_tag="OHN27_003534"
     CDS             complement(13323..>13674)
                     /locus_tag="OHN27_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCQ2287910.1"
                     /translation="GWMGIERVLNQVGPVVGRQMLMLGKRLTAQEAQAANLIDEVVEK
                     EQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYPHQLLAGLMSATQDCQQAT
                     RAFAEKSSVSFNNQ"
BASE COUNT         3413 a         3475 c         3477 g         3309 t
ORIGIN      
        1 tatccgtgtg gaattatttg cgggtcgcgt cacatttaat cataaataat cttgttgtga
       61 ttacttttga aaattagagt gagtgcacaa cattccgggt gtgcggaata cccggttacc
      121 tcttcttcag gagatcgtta tggagatcct ctacaacatc tttaccgtgt tttttaacca
      181 ggtcatgacc aatgccccgt tgttgctggg tattgtgacc tgtctgggct acatcctact
      241 gcgcaaaagt gtcagcgtta ttattaaagg cacgattaaa accataattg gtttcatgtt
      301 gttgcaggca gggtccggca tcctcaccag caccttcaaa ccggtggtgg cgaaaatgtc
      361 cgaagtctac ggcattaacg gcgcaatttc cgatacttac gcgtcgatga tggcaaccat
      421 cgaccgcatg ggcgatgcct atagctgggt aggttacgcg gtgttgttag cgctggcgct
      481 gaacatctgt tacgtgctgc tgcgtcgcat taccggtatt cgtaccatca tgctgactgg
      541 tcatatcatg ttccagcagg cggggctgat tgccgttacg ttgtttattt tcggctactc
      601 catgtggaca accattatct gcacggcgat tctggtttcg ctctactggg gcattacctc
      661 caacatgatg tacaagccga ctcaggaagt gacggacggc tgcggtttct ccatcggtca
      721 ccagcagcag tttgcatcat ggattgccta taaagttgcg ccgttcctcg gcaaaaaaga
      781 ggaaagcgtt gaagacctca aactgccagg ctggctgaat attttccacg acaacatcgt
      841 ctccacggcg attgtcatga ccatcttctt tggtgccatt ctgctctcct tcggtatcga
      901 caccgtgcag gcgatggcag gcaaagtgaa ctggacggtg tacatcctgc aaactggttt
      961 ctcctttgcg gtggcgatct tcatcatcac tcagggcgtg cgcatgtttg tggcggaact
     1021 ctctgaagca tttaacggca tttcccagcg cctgatccca ggtgcggttc tggcgattga
     1081 ctgtgcggct atttatagct tcgcgccgaa cgccgtggtc tggggcttta tgtggggcac
     1141 tatcggtcag ctgattgcgg ttggcatcct ggtcgcctgc ggttcctcga tcctgattat
     1201 tcctggcttt atcccgatgt tcttctccaa cgccaccatc ggcgtgttcg ctaaccactt
     1261 cggcggctgg cgtgcggcgc tgaagatttg tctggtgatg gggatgatcg aaatctttgg
     1321 ttgcgtctgg gcggtgaaac ttaccggtat gagtgcctgg atgggtatgg cggactggtc
     1381 gattctggca ccgccgatga tgcagggctt cttctccatc ggtatcgcct ttatggccgt
     1441 catcattgta attgcactgg cttatatgtt cttcgctggc cgcgcactgc gcgcagaaga
     1501 agatgcagaa aaacaactgg cagaacagtc tgcttaataa ggagttttga ttatgaccgt
     1561 acgtattctg gctgtgtgtg gcaacggaca aggcagttcc atgatcatga agatgaaagt
     1621 ggaccagttt ttaacccaat caaacattga ccacacggta aacagctgcg cggttggcga
     1681 atacaaaagc gagttgagcg gcgcggatat catcatcgct tctacgcaca ttgcgggcga
     1741 aatcaccgtg accggcaaca aatacgtggt tggtgtgcgc aacatgctct ctcctgccga
     1801 ctttggcccg aaactgctgg aagtgatcaa agagcatttc ccgcaggatg tgaagtaagg
     1861 acgcgccatg aaattacgtg attcgctggc ggagaataaa tccatccgcc tgcaggctga
     1921 agctgagaca tggcaggacg cagtaaaaat cggcgttgac ctgctggtgg cggcggatgt
     1981 tgtcgagccg cgttactacc aggcgattct ggatgcggtt gaacagcacg gtccgtactt
     2041 tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg gaagagggcg ttaagaaaac
     2101 cggttttgcg ctggtgacgc tgaaaaagcc tctggagttc aaccacgaag ataacgaccc
     2161 ggtggatatc ctcatcacca tggcggcggt cgatgccaac acgcaccagg aagtgggcat
     2221 catgcagatc gtcaacctgt ttgaagatga agaaaatttc gaccgtttac gtgcctgccg
     2281 taccgagcag gaagtactgg atctcattga ccgcaccaac gcggcagctt aagaaggaat
     2341 tgaagatgtc attaccgatg ttacaagtcg cgctggacaa ccagactatg gatagcgcct
     2401 acgaaaccac tcgcctgatt gccgaagaag tcgacattat cgaagtgggt accattctgt
     2461 gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc gctttacccg cacaaaatcg
     2521 tactggcaga cgccaaaatt gccgatgcgg gcaaaatcct ctctcgtatg tgcttcgaag
     2581 ccaacgctga ctgggtgacg gtaatttgct gtgcggatat caacaccgcc aaaggcgcgc
     2641 tggacgtagc aaaagagttt aacggcgacg tgcagatcga actgaccggc tactggacct
     2701 gggaacaggc gcagcagtgg cgtgacgcag gcattcagca ggtggtttat caccgcagcc
     2761 gtgacgcgca ggccgcaggc gtagcgtggg gcgaagcgga cattaccgcg atcaaacgtc
     2821 tttccgatat gggcttcaaa gtcaccgtca ccggtggcct ggcgctggaa gatctgccgc
     2881 tgttcaaagg tattccgatt catgtgttta tcgctggtcg cagcatccgt gatgccgctt
     2941 ctccggttga agctgcacgt cagttcaaac gttccatcgc cgaactgtgg ggctaaggag
     3001 cggatatgtt gtccaaacaa atcccgcttg gcatctatga aaaagcgctc cccgccgggg
     3061 agtgctggct ggaacgcctg caactggcaa aaacgttggg cttcgatttt gtcgagatgt
     3121 cggtagatga aacggacgag cgcctgtcgc gcctcgactg gagccgcgag cagcgtctgg
     3181 cgctggtgaa cgcgattgtt gaaaccggcg tgcgcgtgcc gtccatgtgc ctttctgctc
     3241 atcgtcgttt cccgctgggc agtgaagatg acgcggtgcg ggcgcagggg ctggagatta
     3301 tgcgtaaagc tatctagttc gcccaggatg tcggtattcg cgtgatccaa ctggcgggct
     3361 atgacgttta ctatcaggaa gccaataacg aaacgcgtcg tcgtttccgt gacggcctga
     3421 aagagagcgt tgagatggca agccgcgcgc aggtgacgct ggcgatggag atcatggatt
     3481 acccattgat gaactccatc agcaaggcgc tgggctacgc gcactatctc aacaacccgt
     3541 ggttccagct ctacccggat atcggcaacc tctcggcgtg ggacaacgac gtgcagatgg
     3601 agttgcaggc cggaatcggg catatcgtcg cggtacatgt gaaagacacc aaaccgggcg
     3661 tcttcaaaaa cgtgccgttt ggcgaaggcg tggtggattt cgaacgttgt ttcgaaacgc
     3721 tcaaacagag tggctattgc gggccgtacc tgattgagat gtggagcgaa acggcggaag
     3781 acccggcggc agaagtggcg aaagcgcgtg attgggtgaa agcgcgcatg gcgaaagcgg
     3841 gcatggtgga ggcggcataa tgcaaaagct aaaacagcag gtatttgaag ccaacatgga
     3901 gctgccgcgc tacgggctgg tgacctttac ctggggcaac gtcagcgcta tcgaccgcga
     3961 acgcgggctg gtggtgatca agcccagcgg cgttgcctac gaaaccatga aagcggccga
     4021 tatggtggtg gttgatatga gcggcaaggt ggtggaaggg gagtatcgcc catcttccga
     4081 caccgcgacg catctcgaac tctaccgtcg ttacccgtcg cttggcggca ttgtccatac
     4141 ccactccact catgccaccg catgggcgca ggcggggctg gcgatcccgg cgttaggcac
     4201 cacgcacgct gactacttct ttggtgatat cccgtgcacg cgcggattaa gcgaagaaga
     4261 ggtgcagggt gagtacgaac tgaacactgg caaagtgatt atcgaaacgc tgggcaacgc
     4321 cgagccgctg catacgccgg gaattgtggt gtatcagcac ggaccgttcg cctgggggaa
     4381 agatgcccac gacgcggtgc ataacgcggt agtgatggaa gaagtggcga aaatggcgtg
     4441 gattgcgcgc agtattaatc cacaactcaa tcacatcgac agcttcctga tgaataaaca
     4501 cttcatgcgt aaacacggtc ctaacgctta ttacgggcag aagtagaaca cgcgctgcgg
     4561 aaatttcctt cctcgggaga taactggtct aattccgcag ccgtttttca aaaaaagccc
     4621 cctgagaagg gggcaaagca aactatggca atgtttcttt ggttatacct ggtgctagcg
     4681 ataaatatcc gcgctggcgt gcatattgcc gttactcccc ggttcccgca tcagaatcac
     4741 gtggtagtac gttgcgccct gcgcatctgt ctcttcattt aatgcctgac gcgcttcgct
     4801 ttcagtggcg aaattatgat tgatataaat cacgcctaag ctttgcacat cgtccatatt
     4861 tctggcctgg tggttattaa tttcaatggc tgcccatgta tttgcactta gcaaaagcac
     4921 agccagaagg gctaaaacac ggctgaacat agatacctcc tcgacggctg tttttgtgtg
     4981 ctctcctcat gtggtgatgg tctgatttaa ttttaatcac ggatgaaaaa gttgatggcg
     5041 accatttctg atgcagttgt tcaaaaaaac agcatgatga agtgtgatga acttcaaatc
     5101 agcggcttag tggttatttg cgaaagggga gatttatttc tgctctgccc ttgagtttag
     5161 caaggtatgt cagtactata acgcgtcatt tttcagccga cctttaacac gttccttgcc
     5221 tccccgggat tcggctgacc cagacaggag gctgaataat ccgtaaggag caattcgatg
     5281 cgtcattacg aaatcgtttt tatggtccat cctgaccaga gcgaacaggt tccgggcatg
     5341 atcgagcgct acactgctgc catcactggt gcagaaggca agatccaccg tctggaagac
     5401 tggggccgcc gtcagctggc ttacccgatc aacaaactgc acaaagcaca ctacgttctg
     5461 atgaacgttg aagctccgca ggaagtgatc gatgagctgg aaactacctt ccgcttcaac
     5521 gatgccgtta ttcgcagcat ggttatgcgt accaagcacg ctgttaccga agcatctccg
     5581 atggttaaag cgaaagacga gcgccgcgag cgtcgcgatg atttcgcaaa cgaaaccgca
     5641 gatgatgcag atgctgggga ttctgaagag taatttctga tgaccaaccg tctggtgttg
     5701 tccggcaccg tgtgcaggac tccccttcga aaggtcagtc catcaggaat tcctcactgc
     5761 cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg gttttcaccg gcaggcgtgg
     5821 tgccaaatgc ccgttattgt tagcggacac gaaaaccagg ccattactca cagtataacg
     5881 gtcggcagtc gcataaccgt tcaggggttc atttcatgcc acaaggcaaa gaacggactg
     5941 agcaaaatgg ttttgcatgc cgagcagatt gaattgatag attctggaga ctagccatat
     6001 ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg ttcaagagat
     6061 cgactataaa gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta agattgtccc
     6121 aagccgtatc accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg ctatcaaacg
     6181 cgctcgctac ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg tcacggtcca
     6241 ttaatacgac tttgagagga taaggtaatg caagttattc tgcttgataa agtagcaaac
     6301 ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa cttcctggta
     6361 ccgcagggta aagctgttcc agctaccaag aaaaacattg aattcttcga agcacgtcgc
     6421 gctgaactgg aagctaaact ggctgaagtt ctggcagctg ctaatgctcg cgctgagaaa
     6481 atcaatgcac tggaaactgt taccatcgcg tctaaagctg gcgacgaagg taaactgttc
     6541 ggttccatcg gtactcgcga catcgctgac gctgtaactg cagctggcgt tgaagtggct
     6601 aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca ctggcgaaca cgaagtgagc
     6661 ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc tgaataattc
     6721 gttattcaac gagacgtaaa aacgccgacc attggtcggc gttttgcttt tgtagcgagc
     6781 acttgcagat atcgataagg gccacaataa gcagccctac gaagataatt acttagctgc
     6841 ctgaatatca gccagcattg gggcaagggc tgggttgtcc ttagcgaagt ttgcgtacat
     6901 tggctgtact ttctcgataa acggagcttt atctacctgg tagactttca cgcccatttt
     6961 atccagtttt gcaaattctt gtttctcttc ctcgctccac aagtctttca tgtagctcat
     7021 tgattcttta gctgctttta atataactgc ctgattttcc tggctaagtt tatcaaacgc
     7081 agcattgctc atcagaacca catcgggaac catagtgtgt tcgtcaaggc tgaaggattt
     7141 tgccacttca ccgtgacgca tggtagtcag cgccatcacg ctgttttctg ccatatcgac
     7201 cacaccctgc tgtaaggctg tatacaactc gccgtaatcc agtggcgtcg ggttaccgcc
     7261 catgactttg atcatttcaa ctgcgccagg gcttggctgg acacggactt tcatgccttt
     7321 cagatcgtct ggtttcagta ctggtttatt gccatagaaa ctgcgggcgc ctccatcata
     7381 aaaagtcagc ccgaaataac ctttgctttt cgttgagtcg aggatttttc tcccgatatc
     7441 gccctgcatg acctgataat aatgatcacg atcgcggaac aaataaggca ggctaaataa
     7501 tttataggaa ttttcaaatg cttccatttc tgccgcattg gtttttacca ggggaatagc
     7561 gccagaacga atcagctcca ttgtttctcg ttgagtaccc aatgttccat ttggataaat
     7621 acgaatagta atatcaccgt tagagtactc tttgctcttt ttcgcaaaga actccatcgc
     7681 tttatgaaca ggatgagact tatcctgatt atgactcagt tttaatgtca ctgcccagga
     7741 ctggcttgcg gataacataa gaacagcacc gataatgcca gcggatactt ttattttcat
     7801 agatgttcct tttctaataa atatggcggc aggtgccgcc agtgaatgcg catcagtagc
     7861 ccagtgctct tggcaatgcc atactgattt gcggaatata ggtcaccatt gccagcacca
     7921 ataccatcac ggcatagaat ggcagcatag gtttgataat tttattaatc ggtaatttag
     7981 atacactaca accgacaaat aacacactgc ctacaggcgg agtacaaata ccgatacaca
     8041 tgttcagtac catgacaata ccaaagtgaa tcgggtccat gcccagttcg gtgacaaccg
     8101 gtaaaaatat cggcgtaaaa atcagtaccg ccggtgtcat atccataaaa gtgccgataa
     8161 tcaacaggat aatattgata ataagcagga ttattattgg gttgtcagaa atagtgttaa
     8221 gcaaatcgag aatcaggaag ggaacgtcag cgtttgacat tgcccatgac atacccatcg
     8281 aagaaccaat taataacaag acaattgcag tggtaataac ggattccaga aatattttag
     8341 gtaaatcttt aactgagatt tcacgataaa gaaccagtgc aaggaaaagg gtataaatta
     8401 ctgcaattgc ggaggcctct gtcggggtaa aaataccact taatacaccc cccataatta
     8461 aaataatcag gaaaatactg gggatagcca tccatatcgc aacgccagcc tggcgtaaag
     8521 tcgggcgttc tggctttgga taaccgcgac gtaccgcgat aataccggct attaccatca
     8581 gtgccagacc cagcaaaatt cccggaacat aacccgccag gaacagtgcg gcaatagaag
     8641 taccaccact gaccagtgaa tagacgatta acgtattact tggtggaata agcaaacccg
     8701 tcggggcaga ggcaacgtta accgcagtac tgaatgccgg gtcgtaaccc tctttaacct
     8761 gttgcggatg catgacgccg cccatcgcag ccgccgaagc cacggctgaa ccagaaattg
     8821 ccccgaacag catattggcg agaatattac agtgggcaag tgagcctggc agacgaccgc
     8881 cgagaatacg ggcaaagttg attaatcgtc gcgctattcc tccatggttc ataatgttgc
     8941 cagacaagat aaaaaatggg attgccaaca acgtaaaact atctaacccg gaggctaact
     9001 tttgggaaac cacactcaat gctgagttga aatcgagttg tgtggaaatt gatgccagtg
     9061 tcgataaacc aatggcataa acaataggta cacctaatgc caacataaaa aagaaggcac
     9121 caaataacac aataatcggt aaccagtaat ccataatttc tccattccta ctatggttgg
     9181 ttgttgtttg ttatctttag agtggttgtt taagttcggt taaaaccata tctatggtgt
     9241 atataataat aagtacacca ctaatcggga tgctcatata gatatatccc atttgccagc
     9301 ctaaagcggg cgaggtctga cctgaatcga atgtatccat cgttaatagc ccaccaccat
     9361 ataccataat gatggttgcg aagataataa cgataatctg aatacacaaa aataaatgac
     9421 gtcgggcttt tcctttcagc atcataggta gcaggtcaat cgccagatgc cggttttgcc
     9481 ctgcggtgta tgccccacca aaaagtccaa gacaaataaa gagcaaacgg ctgagttcat
     9541 ctgtaaaaat actgggacta gtaaatataa atcgtgcagc cacctgccac gtcacacatg
     9601 ccacgatgat cgccaaaatg gtaatgttca atgctgcaag cagagcatta atagatctaa
     9661 ttatatttcg catagtgatt aacctttgca atatcattca tcctgtggct ggtaaattgt
     9721 cccattaaga aaaccaggcc agtttttttc aaaccaaata cctcggataa taggaaactc
     9781 acgaacaggt gtttctctga cgtgaaagat accgttgcaa taatgaataa gtagtttatc
     9841 ttttttatcc tcagtacgct ctgaacgaaa gcgccaggca tcacaaaaac catttcgtgt
     9901 ttgcggaata gaatttccat ccctgtcgag aataatacgt gctccccgac taccaccacc
     9961 tgcgtcaata taatgcgtta attgcgttaa tacggcggca gaagtcagtg ccatatgccg
    10021 ccacataaac agctcggcaa cttcacccac atgtttgata gccaatccat gccgttggac
    10081 aaattcgctc aatagcaggg catcgcgagt ggcgcgtcgg actttatcgg catggcaaat
    10141 aaatcccgca tggtcagaca ttcgtgcctg aattttttct ctcacaaccg acaacggcat
    10201 cccggtcccg ttatcgtgcg cctgagtaat gatctctctt atagaagcca gtgtaggagc
    10261 taccagctgt gctatatctc catcaatatt ccgcttttgc gtgcaaccaa taaaacgtgc
    10321 cagacgaaca gcaaaaacct gcccggcatt caatgctgca ccaccagggc gagtgacgcc
    10381 gtgtgtgcca gcaacttccc ccacggcgaa acagccgggc agggaggttt gtccccagat
    10441 atccacttca atgccgccat tcatatgctg attattcatg gcaaactgca aaggctgtgt
    10501 ggtgagatcg taaccgtgca tcttatacag cgagatagac agcggattca ttcgttgcag
    10561 ccgttcgatg ggcgatggtg ccagagcgtc gttattttcc agatacgcgc gaacgtcgtc
    10621 gtccagtcga tctaatgaga acggcaggtc acccggcacc gcttcaggat tgcgattgaa
    10681 atccataaat acctgacgcc ctgattgctg ctcttgcgct actgccatat ctaacaagct
    10741 ggagccaaaa tccatcaccc gagtggcgtg gaacggccac tgatagcctt tacggaaaat
    10801 gtttgaagcc agctcctggg tggtgcgata gtaatccgcg aggaagttat actcgttacc
    10861 ctcagcatcc acggaataga tatacgggat cacctgtaca taggtgccgg ataaattcca
    10921 cggaaacgtg ctacgcggcg tgcctatacc aaactggctt tcggtcagat tggttagcgt
    10981 caggccttcc tccagcgcca gccccagcga gccaaaacat ttgtgtgggt acacactgtc
    11041 gcgataaagc tcgccaggcc ctcctgttgc cagtaccaca ttgggcgcag tcacaattgc
    11101 cagcccccaa gggttatggg cgcgatgacc ggtcgcgagg attgcccccg caacacggtc
    11161 ttcgccgttt tcgtcacgct gatgcagcag tttaatcact gttgcactgg tcaacactgg
    11221 aatggcgagg cgctgtactt cttccaacag cactttcacc attaaccttg aagtacgcgg
    11281 cccacacgag gttgcacgcc cggcttcgtc atggtcggtt tgataacgaa gaatcgcgcc
    11341 atagcgatct tccggtaatt ccagaccaag atattgcaac ccgccaagag tgtgcagaga
    11401 gcctaccgct tcgacataag cggtgtcgtg atccatcgcc ccaccgctcg ccagtgcctc
    11461 tgccagtttg gtgaagttgt cgccgttgcc cgcggtagcg gcggtaaaca gtgtctgttt
    11521 atcggaacca gaacatgccg acgtccccat atataacccg gcggtggcga tcagcacatt
    11581 ttgctggcgg cgtttaagtt ccacagctgc acgcagcccg gctgcaccac tgccgactac
    11641 cagggcatct gtttgccaga gtgggtaatc aacaccgtct accgtgacaa cccatgcggg
    11701 ctctgcctgt gcaaaaccct ttgggaaagt aacgtgcgtc agcgcatcaa ggatggtgtc
    11761 atcaagctga ctcatgcatc ctccatcggc gtaaacaggc tactttccat caggcgtggg
    11821 tgcttgataa ttggacggaa agccatatac ggcagaatgt cgcgctcaat ctccacaccc
    11881 ggcgcgattt caattaattc aacgccttct gccgttaact ggaatactgc gcgttcagtg
    11941 atatacaaca cttcctgacc actttgctgt gcgtaggcgg cgctaaaagt gattttttcc
    12001 accttggtaa ccagtttagg gatttgacct gactgggcga tatgcaagcc atctggtgtt
    12061 atatcgatct tgctgccttt agcgtcgaac gtgccgcaga acaccacttt acgggcattt
    12121 tgcgcaattt cgagaaatcc gccggggcca atcagattgc cattgagatg ggagacgttg
    12181 acgttaccgt actgatccat ctctcccatg ccgaggaagg tgatatcgat ccctccgcca
    12241 ctgtagaact caaattgatc gagtgatgga ataatggcat cggcgttgcg ggcgcagccg
    12301 aaaaacgcat catccagcat catgccgtta tgtacgcctt gctcgacgct cagccataaa
    12361 ctttggtaag gaacgccttc gcgcagcgct actcctggaa tgccgccggg aataccaaag
    12421 ccaaagttgg ttgaagcgcc agctaccagt tcgcgtgctg cccgacgggc aatcaggcga
    12481 cgtaccggat gactaaccag ttcaatagcg tcgttagaac tcagacggcg atgttggccg
    12541 ctgatggtca ggtcgtaacc gccaagatag gtttgtggct gttcgcggtg ttcaacgatg
    12601 ccatcaacca gaatgcccgg taatttaacc cggcgtggtt caatcgcgcc agcttccagc
    12661 acatcgcgta cctgtacgaa cactttgccg ccgctgttgt gtgctgccag cgccatggaa
    12721 tagctatcca tatcaatcgc ttcttcttca aggctgacgt tgcctcgagg atcggcataa
    12781 gtgccacgca aaatcgcgta atccactttg aaagggcgat aacgaagttt ggtttcaccg
    12841 tcgatttcaa tcagttctac cagctcgtcg gtagtgcatt cattcgactt accgccgcca
    12901 ttgcgtggat caacaaacga tcccagccca acgtgggtaa aaagccccgg acgtccggcg
    12961 ccgatctccc gtagcagtgc ctgaatcacg ccaccaggaa agcagtaggc ttcaatcgtg
    13021 ttatttttta ccagcgcctg cattttgggc gaccaggtaa agtgcccggc aataatgcgt
    13081 ttgagcatct ctgcgtgcgc aaaacgatta gtgcctttgc agtcgcgatc gccaataccc
    13141 agcgagtgga ttagcgtcag gtcgcgaggg tgtccggttt gcaggaaacg cgcttcaata
    13201 gcagccagaa tatggtcggc ttccaccata ccgccgccgt taccgctaat agcaatagtc
    13261 gcaccgtcct gaatttgcgc cgccagtgcc tcggcggttg ttatcttacg catacaattt
    13321 ccttattgat tattgaagct cacgctactt ttttcggcaa aggcgcgcgt cgcctgctgg
    13381 caatcctgag tggcggacat taatccggcc agtagctggt gcgggtaatc ggcatgttta
    13441 ttttccagtg ccagaatcag ttgcttaata tgtgcaagcg ccaccggacc acatttttcc
    13501 agctgcgcta actggttcgc catccagctt tccacctgtt ctttttctac cacttcatca
    13561 attaaattcg ccgcctgagc ttcctgcgcc gtcaggcgtt tacccagcat cagcatctgg
    13621 cgaccgacca ccggaccaac ctgattaagc acacgttcaa tgcccatcca gcca
//
LOCUS       DAJOSY010000109        13667 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJOSY010000109 DAJOSY010000000
VERSION     DAJOSY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.109"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            231..680
                     /gene="rpiB"
                     /locus_tag="OHN27_003535"
     CDS             231..680
                     /gene="rpiB"
                     /locus_tag="OHN27_003535"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HCQ2287911.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            749..1078
                     /gene="yjdP"
                     /locus_tag="OHN27_003536"
     CDS             749..1078
                     /gene="yjdP"
                     /locus_tag="OHN27_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCQ2287912.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(1225..1983)
                     /gene="phnP"
                     /locus_tag="OHN27_003537"
     CDS             complement(1225..1983)
                     /gene="phnP"
                     /locus_tag="OHN27_003537"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCQ2287913.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(1985..2419)
                     /gene="phnO"
                     /locus_tag="OHN27_003538"
     CDS             complement(1985..2419)
                     /gene="phnO"
                     /locus_tag="OHN27_003538"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCQ2287914.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(2406..2963)
                     /gene="phnN"
                     /locus_tag="OHN27_003539"
     CDS             complement(2406..2963)
                     /gene="phnN"
                     /locus_tag="OHN27_003539"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCQ2287915.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(2963..4099)
                     /gene="phnM"
                     /locus_tag="OHN27_003540"
     CDS             complement(2963..4099)
                     /gene="phnM"
                     /locus_tag="OHN27_003540"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCQ2287916.1"
                     /translation="MIINNVKLVLENEVVSGSLEVQNGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSLGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            complement(4096..4776)
                     /gene="phnL"
                     /locus_tag="OHN27_003541"
     CDS             complement(4096..4776)
                     /gene="phnL"
                     /locus_tag="OHN27_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCQ2287917.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(4887..5645)
                     /gene="phnK"
                     /locus_tag="OHN27_003542"
     CDS             complement(4887..5645)
                     /gene="phnK"
                     /locus_tag="OHN27_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCQ2287918.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5642..6487)
                     /gene="phnJ"
                     /locus_tag="OHN27_003543"
     CDS             complement(5642..6487)
                     /gene="phnJ"
                     /locus_tag="OHN27_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCQ2287919.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(6480..7544)
                     /gene="phnI"
                     /locus_tag="OHN27_003544"
     CDS             complement(6480..7544)
                     /gene="phnI"
                     /locus_tag="OHN27_003544"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCQ2287920.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPGEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7544..8128)
                     /gene="phnH"
                     /locus_tag="OHN27_003545"
     CDS             complement(7544..8128)
                     /gene="phnH"
                     /locus_tag="OHN27_003545"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCQ2287921.1"
                     /translation="MTLETAFMLPVQDARHSFRRLLKAMSEPGVIVALHQLKRGRQPL
                     NIATTSVLLTLADNDTPVWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(8125..8577)
                     /gene="phnG"
                     /locus_tag="OHN27_003546"
     CDS             complement(8125..8577)
                     /gene="phnG"
                     /locus_tag="OHN27_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCQ2287922.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVQGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8578..9303)
                     /gene="phnF"
                     /locus_tag="OHN27_003547"
     CDS             complement(8578..9303)
                     /gene="phnF"
                     /locus_tag="OHN27_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCQ2287923.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(9324..10103)
                     /gene="phnE"
                     /locus_tag="OHN27_003548"
     CDS             complement(9324..10103)
                     /gene="phnE"
                     /locus_tag="OHN27_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCQ2287924.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISCSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(10209..11225)
                     /gene="phnD"
                     /locus_tag="OHN27_003549"
     CDS             complement(10209..11225)
                     /gene="phnD"
                     /locus_tag="OHN27_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2287925.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(11250..12038)
                     /gene="phnC"
                     /locus_tag="OHN27_003550"
     CDS             complement(11250..12038)
                     /gene="phnC"
                     /locus_tag="OHN27_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCQ2287926.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(12171..12614)
                     /gene="yjdN"
                     /locus_tag="OHN27_003551"
     CDS             complement(12171..12614)
                     /gene="yjdN"
                     /locus_tag="OHN27_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCQ2287927.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(13174..13509)
                     /locus_tag="OHN27_003552"
     CDS             complement(13174..13509)
                     /locus_tag="OHN27_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCQ2287928.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         2725 a         3796 c         4146 g         3000 t
ORIGIN      
        1 taaaatttta tttcatttaa ttagtttaaa aacatgatgt tatttgtttt tctggttttt
       61 tagatgatag atgttgctgg atggtgagtg tctttaacgc tattgcgctg tcagatgtaa
      121 ctctgtaaaa cagatcagga aggcgtaatt catcgatttt gggtgttttt aattcattaa
      181 catcacaaat gttttttgat tgtgaagttt tgcacggacg gggaagatga atgaaaaaga
      241 ttgcatttgg ctgtgatcat gtcggtttca ttttaaaaca tgaaatagtg gcacatttag
      301 ttgagcgtgg cgttgaagtg attgataaag gaacctggtc gtcagagcgt actgattatc
      361 cacattacgc cagtcaagtc gcactggctg ttgctggcgg agaggttgat ggcgggattt
      421 tgatttgtgg tactggcgtc ggtatttcga tagcggcgaa caagtttgcc ggaattcgcg
      481 cggtcgtctg tagcgaacct tattccgcgc aactttcgcg gcagcataac gacaccaacg
      541 tgctggcttt tggttcacga gtggttggcc tcgaactggc aaaaatgatt gtggatgcgt
      601 ggctgggcgc acagtacgaa ggcggtcgtc atcaacaacg cgtggaggcg attacggcaa
      661 tagagcagcg gagaaattga gattcatcca ctacttgcat ggatgagtaa tgattaatgt
      721 ggatagagtt tctttttgag gttggctaat gaaacgcttt ccgctatttc ttttattcac
      781 cctgctcacg ctgtccaccg ttccggccca ggccgatatt atcgacgaca ccattggcaa
      841 tattcagcag gcgattaatg acgcttataa ccctgaccgt gggcgagact atgaagattc
      901 gcgcgacgac ggctggcagc gtgaagtgag cgacgatcgg cgcagacaat atgacgaccg
      961 ccgccgccag tttgaagatc gccgcaggca gctggacgat cgccagcgcc agcttgatca
     1021 ggagcgtcgg caactggagg atgaagagcg gagaatggaa gatgagtatg ggcgatgagg
     1081 ttgggtatgg agcaggcatc gcccctcacc ccaatgggga gagggagaaa acgagcgcaa
     1141 tattcaatat cgagcacaat cggtcccctc gcccctctgg ggagagggtt agggtgaggg
     1201 ggccagcgcg cacattccct ctcatcacgc caccccaatc tccatcccat caaaccccac
     1261 ctcaaaccct gacggtagtg cgttttccat cagccacgca tcaaactggt ggctgatatg
     1321 ggtcagaatc acccgtggcg agcggataac ctgattcagc gcaagcacgg tatttaaatc
     1381 acagtgatta cgcggtgcat ccgcgcgcgg cgggtgactg caatccatca ccattacctg
     1441 cggctgatta ttgcgtaaaa atttcagcgt tttttccggc aagcctgcgg tgtcagacag
     1501 ccacgccacc cggctgtgtg ccgtttccag cagataaccg aaggtcagtt ttgagtggtt
     1561 gagcggcagg ggcgtgacct gtaacccctg caaatcaaac accacaaacg gttccaccgt
     1621 gtggctgaaa tcaagcaggc ccggatgttt aaacagatcg tcgcagccct gttcatccgg
     1681 cgggccgtaa accgggatcg gatcgccaac gccccagcgc agcggaaaca gcccctggac
     1741 gtgatccata tgataatgcg tcagcaaaaa ctgctggaac gatccgggcg accagcgatc
     1801 ggcgagatcg tgcagcccgg cgtcgatcag ggtgattgcg tcgttaaact tcactacgcc
     1861 gctgcacggt tggcggcgat actgcggcga gcgccgcgct ctggcgcagg ccgcacactc
     1921 gcagccccat gccggaacgc cctgtgcgcc gccggtgccg gtgagcgtga gggtcaggct
     1981 catgttacag cgccttggtg aagcggaagt ggctctgctc gtagccttcg cgcagataga
     2041 aacggtgcgc gtcgtggcgc ttcacgttgg tcgaaagttc ggtcatttcg gccccggcct
     2101 ggcgggcttc ttcttctgcc cacgccagta acttactgcc gacgttcaga ccgcgcgcct
     2161 gcggcattac caccaactcc tgaatttcgc cgatccagtt gacatgatgc agatgaaact
     2221 gcaaatgcag gccgatcatg ccgacaactt cgccatcaag cagcgccaga tggtagcgca
     2281 tgtttgggtc gcgcagattg gcgttaaaac ccacgcgaaa cgcgtggtgg tcaaactccg
     2341 cctgttttag ctcacaaatc agcgcgtaaa ccgcgtcggt gtcgtactgc gtggccgggc
     2401 gaagctcaca agcaggcatg gtgtttctcc ttctgatgga tcagcgtcag cagcgtgtcg
     2461 accgactggc gcaggctgcc gtcattgttg agcgtatggc aatcctgtgg agtatagcgg
     2521 gcggcgcgcg ccaggcgggc gttaatttca ctggcgtttt cacggccacg gttttccagg
     2581 cgctggcgga ggatctccgg cgaaacctgt aaacagacgg gcagcagcgc cgattgatag
     2641 cgcgcccgcg cctgcggcag atgggcgcgt gagccgttga ccagcacgtc gaatccggcg
     2701 tgcagccaga gatcaatctc gacgccgacg ccataataca gaccgttagc gtgccagctt
     2761 aaggccaaca gattttgccc cgcgcgggta aaaaactcct gctcgctcag ggcgatatgg
     2821 ttttcacttc cggcgctggc atcgcgcgtg atgtagcgat gcgccaccag taactgagtt
     2881 tgttcccgca ggcggagttc cgccagcagg ctgtctttcc cggagccgga cggccccatt
     2941 aaccaaatca gttttcccat catcagaaca cccttttacc ctgacgccag acgtggtcga
     3001 tatgaatatg attgtccttg cgatgcgcca gcaccaggtc ggcgcgtttg ccctcgccaa
     3061 tcaccccgcg atcctggaga ttaagcgcct gcgctggatt tttagtcacc agcttcaccg
     3121 cctgcggcag cgtaaagcgg ttgctctcgt catcggcgac gcgaaatgcc gcatcgagca
     3181 ggctggcggg gtagtagtcg gaagagagga tatccagcag gccaagctgc gccagttcac
     3241 tggccgccac gttgccggag tgcgagccgc cgcgcacaat attcggcgcg cccatcagca
     3301 cgttcatgcc atgcttgcgc gaggcttccg ccgcttcgaa cgtggtggga aattcggcga
     3361 tcacgctgcc aagctggtga gattcagcaa cgtgggcgtg ggtggcgtca tcgtggctgg
     3421 caagcgcaat ttttcgcgcg cggcacaggg cggcgatgga ttcgcgattc ggctgcgacc
     3481 agcgtgcggc gagcgccagt tgctcttctt cgtactgctg catctgcgca tcagtgaggg
     3541 agtatttgcc ctgataatat tcgcgatact tctcgcggtt ggcgaactgg cgctggccca
     3601 gcgagtggtc catcagcgac accagcgtca ccggctcgcg ctgcaccagt ttttcaaaca
     3661 gcggcagcgt ggtgtgatgc ggcagttcgc agcgcagatg cagacggtgc tcggcgcggt
     3721 tgacgccgcg tttctgcgtc tcttcgatgg cgttgatcat cttctccaga ttctccagcc
     3781 gatcgccgcc gtcgcgcacg tcgccaattg ccacggcatc cagtacggtg gtgatgccgc
     3841 tcgccaccat cagcgcgtcg tggctgctca tcgccgagtg ggcaggccag tcaactttcg
     3901 ggcgcggggt gaagaattta tccagattat cggtatgcag ctcaatcagc cccggcagca
     3961 gccagccgcc ttcgccgtcc atcgcctccg gcaggcggct ctggctttcg gcaaaggcgc
     4021 ggatttcgcc gttctgcacc tccagcgaac cgcttaccac ctcgttttcc agcaccagct
     4081 taacgttatt gataatcatg aagaggctcc cattgggtgc aggcggtcgg cgacgtcatt
     4141 acgtacagct tcgtcatgga agatgcctac gatggctgcg ccacgggttt tggcttcgcg
     4201 aatcagttcc accaccgcgg cgctgttttt ggcgtccagc gaggcggtag gttcgtcaag
     4261 cagcagaatg gggtagtcga cgataaagcc gcgggcgatg ttgacgcgct gctgttcgcc
     4321 accggaaaat gtcgatggtg ccaggtgcca caggcgttcc ggcacgttca ggcgggtgag
     4381 aagacgcgcg gctttagcgg cgcaggcttc acgcggaacg ccggtatcga gcagcggctg
     4441 catcaccact tccagtgctg agatacgcgg gatgacgcgc agaaactggc tcacccagcc
     4501 gacggtggtt ttgcggattt ccaccacttt gcgcgctggc gcggtgacca ggtctaccca
     4561 ctcgtcaccg tgtttgatct ggatttgacc ttcgtcgggt agatagttgg cgtacagcga
     4621 gcgtagcaga gttgatttgc cgctgccgga atggccgtgg agcaccacgc attcgcccgc
     4681 gttgacggtg agcgaggcgc gattgaggac gggcaggcgc acgccgtttt gctggtgcag
     4741 gatgaaggtt ttactgacgt tttgtacgtt aatcattttt cgcctcgtgg ttcgggtgtc
     4801 ggatgcgacg ctgacgcgtc ttatccgacc tacggggagc gcatttgtag gccggataag
     4861 gcgtttacgc cgcatccggc accggctcaa ttctgcaaaa ccgatgacac cagcagctgt
     4921 gtatacggat gatgcgggtc gtcgagcacg cggtcggtta acccactctc caccacttgc
     4981 ccctgcttca tcaccagcaa acggtccgcc agcaggcggg cgacgcctaa atcatgggtg
     5041 acaatcacca ccgcgaggtt cagctccacc accaggccgc gcagcaggtc gagcaggcgg
     5101 gcctgcaccg acacatccag cccgccggtc ggttcatcca taaacaccag cttcggatgc
     5161 gtcaccaggt tgcgggcaat ctgcaaacgc tgctgcatac cgccggaaaa ggtggtcggc
     5221 aggtcgtcga tccggttggc gggaatctcc acctcttcca gccacttctg cgcggtggca
     5281 cgaatatcgc cgtaatgacg tgccccggtc gccatcagcc gctcgccgat attgccgcct
     5341 gccgacacct ggcggcgcag gccgtcgagt ggatgctgat gcaccacgcc ccattcggta
     5401 cgcagcaggc gacggcggtc ggcctcgctc attgcataca gcgaacggtt ctcgtagtga
     5461 atttccccct gctgcggcgt caggcgcgcg gagatcgact tcagcagcgt ggtcttcccg
     5521 gagccggatt ccccgacaat gcccagcact tcccccggcc ataaatcaaa agagacatcg
     5581 ctaaagcctt tgcccggcgc gtaaaggtgg gtcaggttat tgaccgaaag taacggttga
     5641 ttcattggtt ttttgcctcg ctctgttggc ggcaataatc ggtatcggag cagacaaaca
     5701 tgcggtttcc ggcgtcatcc agcaccactt catcaagata gctgtgggtc gatccgcaga
     5761 tggcgcatgg ctcatcccac tgctgaacgg tgaacgggtg atcgtcgaaa tcgagacttt
     5821 ccacgcgggt aaacggcggc accgcataga tgcgcttctc gcgccccgcg ccgaacagtt
     5881 gcagggcggg catcatgtcc atttttgggt tatcgaattt cgggatcggc gacgggtcca
     5941 tcacgtagcg cccatttacc ttcaccggat aggcgtaggt ggtggcgata tgaccgaagc
     6001 gggcgatatc ttcatacagt ttcacctgca tcacgccgta ctcttccagc gcgtgcatgg
     6061 tgcgggtttc cgtttcgcgc ggctcgataa agcgcagcgg ctcggggatt ggcacctgga
     6121 agataattat ctgatcttcg gtcagcggcg tttcggggat gcggtgacgc gtctggataa
     6181 ccgtcgcatc gtccgtacgt tccgtggtgt ttaccccggt cacgcgcttg aagaagttgc
     6241 gaatcgacac ggcgttggtg gtgtcatccg cgccctggtc aatcaccttc agcacgtcgc
     6301 tttcgccaat cacgctggcg gtaagctgaa tgccgccggt tccccagccg tacggcatcg
     6361 gcatctcgcg cccgccaaac ggcacctgat aaccggggat cgccaccgct tttaagatgg
     6421 cgcggcggat catgcgtttg gtctgctcgt cgaggtaggc aaagttgtag ccgctcagat
     6481 tagccatggt tctgctcctg ttgcagacgt ttgagtagct ccagttcggc ctggaaatcg
     6541 acgtagtggg ggagtttgag gtgtgagaca aagcctgcgg cttcgacgtt gtcggcatgt
     6601 gccagcacga actcttcatc ctgcgccggg cctgtcgcgt gctcgccgta ctccggggct
     6661 tgcagagcgc ggtcgaccag cgccatcgcc atcgctttgc gctcgctcat gccgaacacc
     6721 agcccgtagc cgcgcgtgaa gtgcggcggc tcacccggcg ggtcgataaa accgttaacc
     6781 atttcacact cagtcatcag cagttcgccg acgttcaccg caaatcccag ctcttccggc
     6841 acaatcgaca cgtcgatata gccgctgcgg atctcgcctg cgaacgggtg attgcgcccg
     6901 taaccgcgtt gggtggagta ggccagcgcc agcaaatagc cttcgtcgcc gcgcatcaac
     6961 tgctgcaaac gggaggagcg tgagcagggg taaaccggcg gcgtgcgggt gatgtcatcc
     7021 ggctgtgcgc cgctatcctc ttcaaacttc gccagcccct gacgcgccag caggctgaaa
     7081 acgtgcggcg acggctgctg ttcgctgtcg gcggtggtca gcgtcggcgc ttcgccgttt
     7141 gccagcaggg taaaatcgag caggcgatgg gtgtagtcgt aggttgggcc aagcagctgg
     7201 ccgccgggaa tgtctttata aacggcggag atacggcgtt cgagacgcat cccggtggtg
     7261 tcgagcggct cgcttaccgc cagcttcgcc aacgtggtgc ggtaggcgcg cagcaggaaa
     7321 atcgcttcaa cgttatcgcc gctggcctgt ttcagcgcca gcgccgccag ttcgcggtcg
     7381 gcaatgccgc cttcggtcat cacgcgatct accgcgaggt taagctgctg ttcaatctgg
     7441 gcgacgctca gttcgggcaa atcggtatcg cctcggcgtc ggctctcttg cagggcgtgg
     7501 gcggcgtcga tcgccttctc gccccctttc acggcaacgt acatcagcac acctccacat
     7561 gagtggttcg cggaatagcc agcaggcgct cgccacaggt caggatcagg tcgatgccga
     7621 gcgggaacgg atgcgggcgc tcggtgagtt cgtgcagaat gcactccggc agctgcggag
     7681 cgatcattcg ttcttcggca atacccgcac cagtaaggcg caacatgcgt ccgccgctca
     7741 ggctggcgac ctgtaaaatc agcgtcgcac ccgcttccgg cgcaacggcg gtgccggtgg
     7801 aaagggcgtt gagctgttcg ctggaaatcg cctcatccgt caccgcgaag gtcgcctgtt
     7861 ccggctggct gaccagcggc gcgttggtat gaaaacgcag gctctggttg acgatatcgt
     7921 tatttaatgg ggtagaaagc cacaccggcg tgtcgttatc ggccagcgtc agcagcacgc
     7981 tggtggtggc gatattcagc ggttgccggc cgcgtttgag ctgatgcagg gcgacaatca
     8041 cgcccggctc gctcatggcc tttaacaggc gacgaaaact gtgccgggca tcctgcacgg
     8101 gaagcataaa agcggtttcc agggtcatgc gttgtctccg cgaaccatcg taaagaagtc
     8161 gacccggctg gcgttcactt cggcctggcg tgcggcaatg cgtgccatac ggtcagcgtc
     8221 cagcggggca ataagggttt ctgataagtt ttgaaagtgg cgagattgct gcatcagcgc
     8281 gtcaatcagc gcgcagcgtt cggcgtgctg tttatcacgc ccctgcaccc agctgtagcc
     8341 gagcgtgccg tcagtcaggc gcacggcggc gcgggtcagc gtggcgtcgc cggcaaaaaa
     8401 acgttcgccg gtgccgccca tccgcgcctg aatctgtacc aggccagttt cagcggcgcg
     8461 gatcacctca tagtcggcgg tgatgtttag cgcgttcagg cgtgctgcca gttcagccgg
     8521 ttggctgtgc gccagcacgg acatccagtg ctggcgggtc gcggtatctg cgtgcattca
     8581 gtgctccata gtgaattcaa tcatgtcggc gcgcgtcagg ctgacggagt actccgccgg
     8641 gctgctttca ccgtcacggt ggttaagggt gcgcacgcac agcagcggcg acatattcgg
     8701 gatttcaaga cgctggcact ctttggcctg ggcgcggcgg gcgctgatcc gcgtctggct
     8761 gcggcgcagc gcaattccgg tttgctcgcg cagaaaatcg tgcagcgagc cgctgtcgaa
     8821 gcgttgcagc gtcggccaga gggtgaggtc cgcgaagtag tggtcgatta aacagagcgc
     8881 gacgccgttg acacgacgca gggtgcgcag gtggatgacg ttctccccct cggtaatccc
     8941 cagtgcgtca gcgacgtggc cggacgcggg gcgcaatacc gaaagcagtt tttcgctggt
     9001 gggatggctg ccctgatcca gcagattctg gctaaaacgc gcctgggcgt tgagcgggta
     9061 atcgaacggg cgcatcagca ccagcacgcc gacgccctga cggcgctgta cccagccttt
     9121 ttccaccagt tggtcgatgg cgcggcgcag ggtgtggcga ttcacctcaa agcgcgctgc
     9181 cagttgctgc tcggcgggaa gatagtcgcc gcagcggtag tgttgacgaa gctcctgctc
     9241 aagttttgcg gctatctctt gatagcgtgt tgggtagctg gtcggatgtg tagacaagtg
     9301 catagatatc aatgcctcgc ttatcagata aagtgcttac gcaaccgttg agagaggaaa
     9361 tccagcaggc tgaccgtgac gatgataagc accatcaggg cgcaggtttg ttggaactgg
     9421 aaaccgcgaa tcgcttccca cagggtgacg ccgatcccgc ctgcgccgac catgccgacg
     9481 acggtcgccg agcggacgtt ggattcgaag cgatagaggg agcaggagat cagcagtggc
     9541 atcacctgtg gcagcacgcc gtagaggatc tcttcgagct tgttggcacc ggtggcgcga
     9601 atgccttcca ccgggccagg ttcaattgct tctaccgctt cggaaagcag cttggagagc
     9661 acgccggtgg tgtggataaa tagcgccagc acgccagcga acggtccgag accgacggcg
     9721 accacgaaca gcatggcgaa gaccatttcg ttaatggcgc ggcaggcgtc catcaggcgg
     9781 cgaacgggct ggtaaaccca ccacggcacc aggttttcgg cgctcatcag gccaaagggg
     9841 atggagagaa ccaccgccag cgcggtgccc cagacggcga tttgcagcgt gacggccatt
     9901 tcggtgaggt aatcctgcca ctggctgaaa tcgggcggga agaagtcggc agcgaaggtt
     9961 gccatgttgc cgccgtcttt aatcagcgtg agcggggcca tttccgcgcc ctgccacgag
    10021 acgaccagca cggcgagaac aacggcccag ctcagaagcg agaaccagct gcgcttgggt
    10081 ggggcgatgg tgatggtttg catgtttggc tccggtctgt aggccggata aggtgcttgc
    10141 accgcatccg gcatcaacgc ctgcacattg tcggatgcga cgccggggcg tcttatccga
    10201 cctacgattt actgcaccgc tttactcacc gaactcatcg cgcttagcgc gttgttcagg
    10261 cggtccaggt catccagctg cgcctgaatc tcggtggttt ttgccagctt gtcctgctca
    10321 ttcagtcctt tattgctttt cacgccctgc atctctttaa acagcgcgag ctggcgaatc
    10381 ggcaccagtt gcaggtcgct ggaagcgcgg aatggcgccc agcccaggcg ttccagcacc
    10441 gctttttctt ccggcgtttt gccgtagttc ataaagaagt cgtagatctt gtctttggtg
    10501 gtttcggaaa gattcttgcg ccagacgatc ggatcgcctg ggatcagcgg cgacttccag
    10561 atcaccttca gttctttcag cttctctggc gcggaggttt tcagcttgtc gaggttttcg
    10621 gtgttgttgg tggcaacatc cacctgcttg ttggcgacgg ccagcgcgtt ggtttcatgc
    10681 ccggcgttga cggtgcgctt gaagtcgctg gcggagatat tgtttttggc aaagacgtag
    10741 taaccgggga cgaggaagcc agaggtggag ttaggatcgc cattgccgaa ggtgagatct
    10801 ttccgcttcg ccagcagatc gttcaggttg ttgatcggac tatctttgtt gacgatcaac
    10861 acgctccagt aacccggcga tccgtccgcc gcgacggtct gagcgaagac ctggccgttg
    10921 gcgcgatcca ccgcttccat tgccgacagg ttgccgtacc aggcgatatc cactttattg
    10981 aagcgcattc cctggataat gcctgcgtag tctggggcaa agaaggcgtt caccttcacg
    11041 cccagcttct tctccatatc ctgtaagaat ggcgtccatt gcggtttcag gttttgctgt
    11101 gattccgttg aaataatgcc gaaattcagc gccttttcct gctcttcggc gtgtgccggg
    11161 cttaacaggg tgctgaggct gaacatgctg gtgaaggcca gcgaggcaat tatcttagcg
    11221 ttcattcgtt ttcctcaatg atggggatgt caggcagctt tcgcgttctc ttcgatgcga
    11281 ttaatgctgc ggtagagatg gtcaaaacgt tcgttatcaa actgttggct gctgccgtcg
    11341 tagaaaacgt gcccctggcg cagggcgacg atgcgttcgc agtagcgcag ggcgtaatcc
    11401 acctgatgca gcgtgacgac cacggtgatg ccgtcgttct gattgatgtc gcgcagggtg
    11461 tccatcacga tgcgggcgga ttccgggtcc agcgaggcga tgggttcatc ggccagaatc
    11521 accttcgcct gctgcatcag cgcgcgggca atcgccacac gctgctgctg tccgccggag
    11581 agggtggaaa cgcgttgatg ggcaaaatgc accatgccaa cgcgggtcag cgcctgtaac
    11641 gcgcgttgtt tctgctcgcg ggtaaaccag ctaaaacagg tgcgccagaa cggcgtgctg
    11701 ccgagcgcgc caatcagcac gttctccagt acgctcaggc ggttcaccag gttgaattgt
    11761 tggaagatgt agccggtgtt ggcgcggctt ttgcggatat cgcgcgccag acggccttcg
    11821 cgctggactg tgcggcccag cagctcgata tggctgccgg cggatttatc gccggtaatc
    11881 aaaccgctta agtgacgtaa aagggtggat ttgccggaac ccgacggccc aagcagagcc
    11941 accatttcac cgtgatgaat gttcagatca accgcatgca gcgcctgatg ctgattgaag
    12001 gttttggcga gcttctcgac acggataatc gtttgcatga tgcagcctcc ctaaaaaagt
    12061 ggctccatcg tggcggatta ttgtgacgat ttggttaatt aaaagtgact aacagatgaa
    12121 gagttaacgg gaattcgatg acagtgcggg agggcgggcc tcccggcgga ttattgcgtt
    12181 ggttgttgtt tgacgacatt aatcatccac ggtacgccaa atttatcggt gactttgccg
    12241 aagccatgcg cccagaaagt ttcctgccag gccatttcga tttttccgtt agcggcaagg
    12301 ttatcaaacc agcgttttcc ttcttcgacc tgttgcgaat cgagcaccag cgtaaagccg
    12361 gagtagctgg cttttcctga cggtatagca tcgctcatca tgatatcgct tccggcaatg
    12421 cgcacattgg cgtgggcgat ggcggtatcg ggaaattgca ttccggaagg gcagttctcg
    12481 gcgctgtcct gcgctgattt cggcatttcg ccgaagctga ttttatagag cagttcagcg
    12541 cccaacgtac gttgataata agcaattgcg tcggcacagt taccggcaaa agagaggtag
    12601 ggacttaacg gcatgatggt gacctcagtt aagagaagcc agttaagtgt agttcgaaat
    12661 ttatacagat gagagggaag cctgataaga cgcggtcagc gtcgcatcag gcagtcggca
    12721 ctgttgccgg atgcggcgta aacgccttat ccggcctacg gagggtgcgg gaatttgtag
    12781 gcctgataag acgcgcgagc gtcgcatcag gcagtcggca ccgttgccgg atgcggcgta
    12841 aacgccttat ccggcctacg gatggtgcgg gaatttgtag gcctgataag acgcgcgagc
    12901 gtcgcatcag gcatctggca ctgttgccgg atgcggcgta aacgccttat ccggcctacg
    12961 gagggtgcgg gaatttgtag gcctgataag acgcgcgagc gtcgcatcag gcagtcggca
    13021 ctgttgccgg atgcggcgta aacgccttat ccggcctacg gagggtgcgg gaatttgtag
    13081 gcctgataag acgcgcaagc gtcgcatcag gcatctggca ccgttgccgg atgcggcgcg
    13141 agcgccttat ccggcttacc gatcacaata caatcagttc tttttcacaa actcagattt
    13201 cagtttcatc ggaccaaaac cgtcgatttt gcaatcgatg ttatggtcgc cttcaaccag
    13261 gcggatgttt ttcactttgg tgccaatttt cagcatcgaa gagctacctt tcaccttcag
    13321 atctttaatg atggtaacgc tgtcgccgtc agccagcaga ttgccgttag catctttaac
    13381 gatcagctcg tcgctttcct gtgcaggttc tgcgtcgttc cattcgtagg cacattccgg
    13441 gcagatgtac atgccgttat cttcgtaggt gtattcggag ttgcattttg ggcagtgtgg
    13501 taatgacatg tggatttcct caaaagtcag cagggcaaaa gcgccaaaaa acggcagatt
    13561 gccgaaaaag gccgcaatta tacacaaaat ccctgagttt gtcgggggta ctgctgattt
    13621 tagacgacga tcgttttcat ttgaaaggaa acgcaaaaag aaaataa
//
LOCUS       DAJOSY010000110        13634 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJOSY010000110 DAJOSY010000000
VERSION     DAJOSY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.110"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..3735)
                     /locus_tag="OHN27_003553"
     CDS             complement(<1..3735)
                     /locus_tag="OHN27_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCQ2287929.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLANADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFHDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVNVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSCRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQLPVTYEWLRLEDRTSHSGELPSGGKSFTVNFD
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTQYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVELTSSLKGKPVSAQLTVGV
                     VDEMIYALQPEIAPNIGKFFYPLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSER
                     RVKMLERPRREEVDTAAWMPSLTTDKQGKAYFTFLMPDSLTRWRITVRGMNGDGLVGQ
                     GRAYLRSEKNLYMKWSMPTVYRVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLT
                     LHKGANYISLTQNIQQSGLLSAELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGG
                     ENALMLPEQASNIRLQSSETPQEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLA
                     DHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQA
                     LGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQEMNLPCKTLLKGLDEAIARRG
                     TKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTMTLLK"
     gene            complement(3669..4292)
                     /locus_tag="OHN27_003554"
     CDS             complement(3669..4292)
                     /locus_tag="OHN27_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCQ2287930.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(4289..5977)
                     /locus_tag="OHN27_003555"
     CDS             complement(4289..5977)
                     /locus_tag="OHN27_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCQ2287931.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(6126..8753)
                     /gene="gyrA"
                     /locus_tag="OHN27_003556"
     CDS             complement(6126..8753)
                     /gene="gyrA"
                     /locus_tag="OHN27_003556"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCQ2287932.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELMDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            8900..9622
                     /locus_tag="OHN27_003557"
     CDS             8900..9622
                     /locus_tag="OHN27_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCQ2287933.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(9750..13484)
                     /gene="yfaL"
                     /locus_tag="OHN27_003558"
     CDS             complement(9750..13484)
                     /gene="yfaL"
                     /locus_tag="OHN27_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCQ2287934.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDSHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         3151 a         3785 c         3343 g         3355 t
ORIGIN      
        1 tttcagcaac gtcatggtta agacgtttgc caccgcatct gccagtggag attccggtgt
       61 atcgaggatc aggctgtcat tgatatcgcg gatgtcttcc tcagagaaat cttcatcttt
      121 agttccgcgc cgggcgatag cttcatccaa ccctttcaac aacgttttgc acggcagatt
      181 catctcctgt gcccacgcca gcaccagcgc ccgatgcaat aacggcatgt tatccgcctg
      241 ctcggcataa ctgtcgagca tatgctgcca gtattccggt tgttgcgtta cgccgagcgc
      301 ctggctggcc tgccagtcgg cgtaccatgc ccatgccgta aggaaggcgt caccattgcc
      361 atcttcaccc caccaggtaa agcgcgctcc gggccccgcc agttgcatca gccgcagacg
      421 gttatcctga atcatctgac gaatgtcgtt agcggcggca ctttgatgat cggcaagcga
      481 acgccaggcg agactgagcg ggatcagacg gctaccggtg ttgatcaccc caccccacgg
      541 ttcatcgact aacgcatcaa gattgttgcg gaaaatctcc tgcggcgttt cactactttg
      601 tagccggata ttactcgcct gctcgggcaa catcagcgcg ttctcgccac cgccgagcat
      661 gacatttttc tgctgttcaa cgggccagct gttgtccaca aaagacagtt ttgtgctaat
      721 gctgtcctgc acttgcccat tttgttgcag ttctgcactt aacaagccag attgctgaat
      781 attctgcgtc agcgaaatat aattcgcccc tttgtgcagc gtcagcgtct ggcgcatctc
      841 agcgcctgca aatttagtca ccagcgccac cggttcgtta tcctgctgac tgaagataaa
      901 cagtcctgcc gccggtttgt cgcccacgcg atacaccgtt ggcatactcc acttcatgta
      961 gagatttttt tccgaacgca gataagcacg cccctgcccg accagcccgt cgccgttcat
     1021 cccacgcacg gtgatacgcc agcgggttaa cgaatcaggc atcaggaacg tgaaatacgc
     1081 tttgccttgt ttatcggttg tgagtgacgg catccatgcc gcggtatcca cctcttcacg
     1141 ccgtggacgt tcaagcattt ttactcgccg ctcgctgcgg ttagttgcgc caggcgcaac
     1201 cggctcgctg gagagtgcct ggtcgtagct gataaacgac agactggagc tggtgcgtac
     1261 attgttacgc cccagcggat agaaaaattt gccgatattc ggcgcgattt ccggttgcag
     1321 cgcgtagatc atttcatcga ccacgccaac cgttagctgc gcagaaacag gtttaccttt
     1381 cagcgacgag gttaactcga cattgaccag ttcaccaggc tggtaatggg ttttgtccgt
     1441 tttcacccgg atatcaagct gaggaacggc aactttgatc ccggcgttct gaaaactgta
     1501 ctgaccatta cgggtataca gcaccgaaaa agtgatgtta ggcgcaaagg aattgctcac
     1561 tggaacccgg gcttcatact gggtatcgtt taaacgttgt agcgttagcc agtttgccgg
     1621 atgcgaaagc agcgactgct gttccacgcg atcgcgttcc agcgtcaata atgcttcatc
     1681 aattggctcc ggaaaagtaa tcagcatctt cgcggtttcg cctggctggt acagcgtttt
     1741 atccgccacg atatctaccg taccagtatg cgccgtgctg cccttaccgc tgacggcatg
     1801 acttaaccca gcgagaatta agccgtcttt atcgcgtaat gtcagattgt agttgccagg
     1861 tttatcgaaa ttgacggtaa aggatttgcc gcctgacggt agctctccgc tatggctcgt
     1921 gcggtcttcg agacgcaacc attcatacgt aacaggaagc tgttttgaag attccagcgc
     1981 ggcataacgg aacacaaccg actcgccgct attactgtat tgtgcggcag tacttaatga
     2041 gtaatgcgcc agaccgcgtt caatgaggat ctctttggtg gtggtgacac gatacgccgc
     2101 gccgtcactg gcggagacgg ttaacaaata acggctcggt ttatcggcgg cggggagatt
     2161 taacgccaca tgaccgctgg cgtcggacac cgtttcgctg ccttccagcg acacggggaa
     2221 acgtccggca taacgcaaat cgttaccgac cattgataat tgctgagcgc gcaaacttaa
     2281 ctgcacgcgg gcatttttta ccggctcgcc atccgggtag agcagttgca gtttgccgct
     2341 gaccgcttcg ccagttttga actctttttt gtcgagagct aaaccaatct cgaaatgtgg
     2401 cttgatgtag tttgccacgc gacaactgct gctatagacc tgattgcggt aagcaagacg
     2461 taactcataa cctccggcaa cggcattttc tggcaggcgg aaacttccct gcccgccatt
     2521 gcgcgcatcc agcgtgacat tgacggtttg caacagactg ccgttggcgt ccagcaccga
     2581 aagcttcgcc ggggcgctga cgatggggga tgaatgcaac ggatcgtgga actcgcggcc
     2641 catcacttta acatcgacac gatcgcctgc gcgatatagc gggcgatcgg taaagatata
     2701 caagcgggtg ttgtagattt cactttcgta gaagaagttc tcggagacaa aaacgccgcc
     2761 ttcagcatcc ttacccagaa tgtatgagcg ttctggcgat atatgttgta actgcaaggt
     2821 accgctgtca tcggtcacac cgcgggtcat cacgccaaga ccgtcagtcc acaagatctc
     2881 tgagccgggc ttcgcttcac cctgtttttt acccgcggtc cacaccagaa gctcgttgcc
     2941 tgacactttg ctaagcgcca cggtatcgga aacaaacacc accgtcgtcg cccgataccc
     3001 accaaccatc gcctcgacga ggtacagtcc cggctcttgt tggccgagag gaatataaat
     3061 gttacccggc tgcggcgaga tgaaattgct ggatgcgcct tccagtttta ccccttgctg
     3121 cggctcgacc ggtttagcct gccatagtgg ataacggaac tgttccacca agggatattt
     3181 tttcagcggg gaaaactggt tgttctgtac ataacgggaa ggtttaataa tggcattgcc
     3241 gagatgtaat tcgggtaatg cctgagtcac attctgccgt gactgagaag agaaagtacg
     3301 ctgcattacg cggcgagatt tgccgtacca gttatcccac agccaggtca gcgtattgtt
     3361 cagcccgtcg cccagatatt gcggttgcac cacaatgcga tgcaggtttt tctgctggcg
     3421 caaaaatgcc atcgggtcag gaatacgata caggcgaacg tccacgccgc cgtactcttc
     3481 catctgatag cgccgataat cacgccccgg cgcttccagt cgcactttcg cctcttcact
     3541 gctgctaaaa ctgctgtcag caagcaaaaa gaatgttccc ccggcgggcg gcgcatagtt
     3601 actggaagga agcgaatcat cagcattagc aagccctgtt cccacaagag acagggacaa
     3661 gcacgctgct atcgcgccag aaaatttaaa cgataaatgc caatgaaatt gggattggaa
     3721 tcgttgggta tccatcgggt gtccttccat gtcataagtt gttgcagact gactgcgcgc
     3781 attccgttgt cagttttcgt ggcgcttccg gtgtggtaga tgacgtaacg ccccatccag
     3841 accattaagt gctgggcatc gccctgatcg aaaaaaatca tatcgccagg cagcgcctgg
     3901 tttatgtcct ggccaataaa ctggctgttg tactgaatca aattaatcgc ggtcacgtag
     3961 gggccggttt tcccgttccc ctgattccag ttttgcgcca gttgacgctg tccaggtgtt
     4021 agcgtcatct ctggcggcaa atactggcta gagaaaccgt tactttttag ccatttacta
     4081 tcgtgaactt tcagcgtctc gttcgccgca aatcgcacca ggcccgcaca atcctgctga
     4141 taccagcgtg gacttggccc ctggcggagc tgttcttgtg caatgcgtac aaaccaggcg
     4201 cgaaacagcc cggattgttc gacattcagc atttcgctat gggcaacaac acaacacagc
     4261 cagcaaatca gcgccagcag cccgtgcctc atagcggctg ccaggttatg ggtagccact
     4321 gccaggcggc accgggttcc atctgggcca gcttcatgac ataacgcggt tgttgagata
     4381 aagcgtccag cttcggcatt aataaagttt gtgcggcgtt ataaaaaacc ggttcgagat
     4441 tcttcggcag actggtcaac gtttcgttac gcagcagttt cgccacgcct tgtggattga
     4501 tatagagcgg aacgatgcca tcagtgggta ttacatccac cattgccggg cggtttttat
     4561 tcagtgtttg cagggcgtta ttaaccagcg tgtcatcgag ggagaaaagc agcgttttgt
     4621 tttgcatcgc cagcgacacg cggaaaaaat aatccgacat taattgatcg ggttgcgccg
     4681 cctgcgcttt cggatactga ccgtatcggg aactcacttc gcgacgccag atttgcgctt
     4741 cgccctgctg agtctggctt atcggcaaaa caccttctgg cgctttgctt tcgtgcgcac
     4801 caatattttg cgtaaacagt ttccctggca attgcgcctg ttcggcagtg ccatcaaact
     4861 gaccgacaaa cagcggggtt tgcaattttg agtcttcata ccagcacagc cccgcggcac
     4921 cgtctaacgc cccattcagc ttgtcgtttt cctggctgat gtgcgaaagc atctcttcgg
     4981 caataccgtg tgaatacggc accaccacac agaagctggc cccggcaggc atactgttcc
     5041 agaccggcgt aaaatcgaaa ctggtatcta cgctggcgga ttcatcattt aacgccacaa
     5101 aactgtgcca gccgtcgtta cccatttcga agcgtacgcc agcaaaagaa ggcattaacc
     5161 gctggtagcc aaaacccagc aacctggcgc tgaccacgat gcgctggcgt actggcgttt
     5221 tttcagcagc acgctcttcc aggccaaagc ttgcttgcca gcgttttttg ccgctcaaca
     5281 aatcacctgc gatcgccgtg gcttcggtat cctgctgatc atctttaaac agcatatccg
     5341 tgctggaaaa cactagcatc ttgtcctgat atgtcgcgaa catcagagcg ttattgccgt
     5401 tatagcgcaa ctgataaaca ggaacagttt cactatttat cttgatgcta ctgatttccg
     5461 ttttgcttaa ctggctgtcg ctggtagcgg caaacaacaa tggctccagc agtttgctta
     5521 aaccactgcg ctggatcagc accatataat gtgaaagatg gccttgttta tcgtgccaca
     5581 gcgctgcctg cgcgggctga tctaagagtg acgaaaagag cttatctttc agcgtgagat
     5641 cgtgttcata gacaatacga cgaatgctgc cttcaatgcc cagacgatcg gcatgattct
     5701 gataatagaa aacgaaatct tcgctcagaa catcgtggag aaacggaatg gtgaggagat
     5761 ctttgggaag ctggctcaga gagtcgctgt cgagaaagag gtccggctca ttgagatcga
     5821 tttgcagatt gttgtgcacc accagcggcg acaacgtttt ttctggccca ctgccagcat
     5881 attgcaacgc ccagacgcca gcggaaagca gtgctattgc gccaaaacct acaagaccat
     5941 agaaccgcca gcctttcgcc tttttttcac cactcattgc cacattcctt gtgtatagcc
     6001 agccattttt tacgggcaca gccaaacttt accgtgccct aatacgacaa aagcccagac
     6061 tttgcagcct ggacttttca attcaaacaa gggagatagc tcccttttgg catgaagaag
     6121 taaaattatt cttcttctgg ctcgtcgtca acgtccactt ccggagcgat ttcatcgtcc
     6181 ccttccgcgg cactgccatc gatggtatcc agatcttcct cgtcaaccgg ttcagcaaca
     6241 cgttgcagac ccactacgtt ttcatcttcc gcagtacgga tgaggatcac gccctgggtg
     6301 ttacggccta cgatgctgat ttccgaaacg cgagtacgta ccagcgtacc ggcatcggtg
     6361 atcatcatga tctggtcgca gtcatctacc tgtaccgcgc caaccactaa accattacgt
     6421 tcagtaacct taatggagat aacccctttc gtcgcacgcg acttggttgg gtattccgcc
     6481 actgcggtac gtttaccgta accgttttgc gttgcggtga ggattgcgcc atcgccacga
     6541 ggcacgatca gagagacgac tttatcgcct tcacctaagc gaataccgcg aacaccggtg
     6601 gtgttgcagc ccatcgcacg gacagaagac tctttaaagc gcaccacttt accttcagcg
     6661 gagaacagca ttacttcgtc ttcgccgctg gtcaggtcaa cgccgatcag ctcatcgccg
     6721 tcaaccagtt tgatcgccac tttaccggcg gtacgcagac ggttgaactc ggtgaggaca
     6781 gttttcttca cggtaccgtt agcggtcgcc atgaagactt tcacgccttc ttcaaactcg
     6841 gtcactggca ggatcgcagt gatacgttcg tcctgctcca gcggcagcag gttgacgatc
     6901 ggacgaccgc gcgcgccacg agtggcttcc ggcaactgat aaactttcat cgaatagacg
     6961 cgaccacggc tggagaagca cagaatatgg tcgtgagtgt tcgccaccag cagtcggtcg
     7021 ataaagtctt cttctttaat acgtgcggca gatttacctt tcccgccacg acgctgcgct
     7081 tcgtattcag aaagcggctg atacttaacg tagccctggt gagagagcgt cactaccaca
     7141 tcttcctgag tgatcagatc ttccaggttg atgtctgcgc tgttggcggt gatttcagta
     7201 cgacgtttgt caccgaactg ttcacgaacc agctccagct cttcacggat cacttccatc
     7261 aaacgatcgg cgctaccaag aatacgcaac agttccgcga tctgatccat cagctctttg
     7321 tattcgtcga gcagtttttc gtgctcaagg ccggtcagtt tctgcaaacg cagatccaga
     7381 atcgcctgag cttgctgttc ggtcaggtag tacagaccat cacgcacgcc gaactctggc
     7441 tccagccatt ccggacgcgc agcatcgtcg ccagcacgtt cgagcatcgc ggcaacgttg
     7501 cccagctgcc acggattagc aaccagcgca gttttcgctt ctgcaggcgt cggcgcatga
     7561 cggatcagtt cgatgatcgg gtcgatgttc gccagcgcca cggctaatgc ttcaaggata
     7621 tgagcacgat cgcgagcttt acgcagttcg aaaatagtac gacgggtcac cacttcacgg
     7681 cggtgacgaa caaacgccgc gatgatgtct ttcaggttca tgatcttcgg ctgaccatgg
     7741 tgcaatgcca ccatgttgat accgaaagaa acctgcaact gggtctggga gtagaggttg
     7801 ttgagcacaa cttcaccgac cgcatcgcgt ttcacttcaa tcacgatgcg cataccgtct
     7861 ttgtcagact cgtcacgcag cgcgctgatg ccttccacgc gtttttcttt taccagttcc
     7921 gcaatcttct cgatcaggcg cgctttgttt acctgatacg gaatttcgtg gacgataatg
     7981 gtttcacgac cggttttggc gtcaacttcc acttctgcgc gagcgcggat atacaccttg
     8041 ccgcgaccgg tacggtaagc ttcttcaata ccgcgacgac cgttaatgat tgccgccgtc
     8101 gggaagtccg gccccgggat gtgttccatc agcccttcaa tgctgatgtc ttcatcatcg
     8161 atatacgcca gacaaccgtt gatgacttcc gtcaggttgt gcggcgggat gttggttgcc
     8221 atacctacgg cgataccgga agaaccgttc accagcaggt taggaatttt ggttggcatg
     8281 acgtcgggaa ttttttccgt gccgtcatag ttatcaacga aatcgaccgt ctctttttcg
     8341 agatcggcca tcagttcatg ggcaattttc gccagacgga tttccgtata acgcattgcc
     8401 gccgcagagt cgccgtcgat ggaaccgaag ttaccctgac cgtctaccag catgtaacgc
     8461 agcgagaatg gctgcgccat acggacgatc gtgtcataaa ccgccgagtc accatgggga
     8521 tggtatttac cgattacgtc accaacgaca cgggcagatt ttttataggc tttgttccag
     8581 tcattgccta gtacgttcat ggcgtaaagt acgcgacggt gtaccggctt caggccatct
     8641 cggacatctg gcagcgcacg gccaacaatg accgacatcg cataatccag ataggagctc
     8701 ttcagctctt cctcaatgtt gaccggtgta atttctctcg caaggtcgct catctaaccg
     8761 ctatccctct actgtatccc ggattcaaag gtcgcaaatt ataacacagc cgcgcagttt
     8821 gaggtaaacc tatacgcttt attcacatcc aatgcctgat atactcgttt gtcttgccaa
     8881 ttacggagta gaagtgccaa tgaatgccga aaaatcgccg gaaaaccata acgtagacca
     8941 cgaagagatc gctaaatttg aagccgtcgc ctcccgctgg tgggatcttg aaggtgagtt
     9001 caaaccgttg caccgcatta acccgctgcg tctgggctat attgccgagc gtgctggcgg
     9061 tttatttggc aaaaaggtgc tcgatgtcgg ttgtggcggc ggcattctgg ccgagagtat
     9121 ggcgcgcgaa ggcgcgacgg tgaccggtct ggatatgggc tttgagccat tgcaggtggc
     9181 aaaactgcac gcgctggaaa gcggcattca ggtggattat gtgcaggaaa ccgtggaaga
     9241 gcacgcggca aaacatgccg ggcagtatga tgtggtgacc tgcatggaga tgctggagca
     9301 cgtccccgat ccgcagtcag tggtcagagc ctgtgcgcaa ctggtgaaac caggcggcga
     9361 tgtctttttc tcgacgctta accgcaacgg caagtcatgg ctgatggcgg tggttggcgc
     9421 ggaatatatt ttgcgcatgg tgcccaaagg cacgcatgat gtgaagaagt ttattaaacc
     9481 ggcagaactg cttggctggg tagatcagac aagccttaaa gagcgacata taaccgggct
     9541 acattacaac ccgctcacta atacttttaa actcggcccc ggcgtggatg tgaactatat
     9601 gctgcacacg cagaataagt gaggttgttg tttggccgag ccaatgcctg atgcgacgct
     9661 tgccgcgtct tatcaggcct acaaatgctc cccgtaggcc ggataaggcg tttacgccgc
     9721 atccggcaac cgtgccgact agacagtgat taccatttca ccgtcatcga caaaaaccct
     9781 gccgtctggg caaaatcatc actccctttc tgccacgcca cgctgccgcg cagggacact
     9841 cgctgactga tattgcccgt gactcccact tttatttcac cccgtcgctt caccgcatcg
     9901 tcactgatag tgctgccatc ttcttcaatt tccgtcgcat ggggatcgtg ataataattc
     9961 agatctaatg ttggtgtgac accaaccgcg gtacgccatt cgctgtgtaa acccagccgc
    10021 gtctgaatat catccccctg cgattgtgac acgcgcgcat ggttagcggc ggtaaaatca
    10081 tcctgctgca cgccctgata aatcacctgc gcctgcggtt caatcaccac accacgcccc
    10141 ggtaaccact gatacccggc ttccagcgag gcgataatcc ccgacgagtg gtaatgatct
    10201 gtgccatcgt cttgttcgga aacatcattg ttaaaccatg cgtattgcag ccagctatcc
    10261 agccaggccc cctgcttctg attaccgtgc tgataccagc ttgaggtcag cccaacggca
    10321 taaccgtggt tctggttatc ggcgcgagtt ccggtcatat tcgagcggct ctcgccctga
    10381 ttatcactgt agccaccaac cgccccaagc atccactcgc catcatcgcc ccagcgcccg
    10441 ctaaacaggt caccgctaag ctgcaccgta gaagtgtctt cctgctgagc cagttgccct
    10501 gccgccgtgt aatgataacg tccgccgata acacgtaaat tcagcgtctg accattgcca
    10561 cctgcatgat cgcgtcgctc catcataaac gcctgatttg ccgcccgcag attattgaga
    10621 taaccgccaa ctttggcatt cagcaccggc tggtaagcgg ggattggatc gggtgttggg
    10681 tcaggatcgg gtgttgggtc cgggtctgga tcaggtggcg atggcggcgt tacttcttgc
    10741 gatcgcagat accagtcgtt gttatcttcc actagcgtgt agtcatacgc tcccatgttg
    10801 acgtagccac tgccagccag actgaactgt gcattgtttt gaaactgcgt gggatcagct
    10861 gcgaaatcaa ccactttaat gcctgtcgat gtcggctcac caatccctgt aatggagtta
    10921 accaccacag ttgtgttgcc agcagtatta ccgttcatca ccaattgatc gcttaccgag
    10981 tcatcgccgt ttaattcgct gtcgaacagt agcgtaccgc cgccggtata atcaccgttt
    11041 accgtcagcg tatcgccagc gacgccattt tgcaggctaa ccgcaccgga cgtattggtc
    11101 agatcaccat taaccgtcac gtcggcaaga taagtcaggc tgtcagtttc aaggttggta
    11161 ttaagctcac ctgtcaacgt cacgccatcg gtagcattca ccagcgtccc atcgccgtta
    11221 aacaatgagg cattaagggc gacggaagta tcctgcccgg tcagacgcaa aaccgtacca
    11281 tcgctgatat cgatagtgcc gctggaagta gcatcgatag actgaatatc ctgatccgcg
    11341 ccagtaacga acgtcgcccc atcaccgacc cacagtgacg aagcataagg aaagatatcc
    11401 gcaacatcgc ctttcagggt gccttcttcg acacgtactg ccgactgcgt tgtaccgctg
    11461 gcggtcagct ccagcgtacc cgcccccgtt ttagtaaatg tgctaccctc atcctgatgc
    11521 tgcccgctgc tgtcagccat cagtgcgccc cactgcgtgt ctacccccgc atcaactgcc
    11581 acttcaccgt cggcgcgcat ttcaatatca cgcccatgct cagtcgtcgt agagcgagta
    11641 tcgacattgg cagtaatctc catcacgctt tcttgttgct tatcggtaaa gataacctgg
    11701 cggttatagt gggtatctcc aagctgcgag ttgtcgctca ccatcagcgt accggaggcg
    11761 atttgtgttg tgcccaaata actattattg tttgccagac ttacctgccc gtcgctcgca
    11821 tcgaccacca cgtagacgcc atctttcccg tcgccgccga tatgcaaatt gtcgccacct
    11881 gcaagatcta ccacatcctg actgccgata accgttccgc tgctgccact gacttcaagg
    11941 ccatcgttgt atgatgtacc gctctgccag gtggagaaat cagagagatc gagcacaccg
    12001 ccgtttaaca cgatcgactg cggatcgtcc tggagagcgg cgagatctgc cgcgtcgcct
    12061 tccagcgaaa gcaccgcacc atcatcaacg actatatcgc cggttagcgc catcgactgc
    12121 gcccccgaca gcacgtagct gccgttttgc gcaatggtga gctgaccagc accttcgatg
    12181 gtgccagcaa aactgccttg attaacagta acattgccac cagcatcgat atttaaagtg
    12241 ccattctgaa agcccgtcaa tgagtgcaca aaggtttgtt gggtggagcc aacgtttagc
    12301 tctgcctgat tctggtactt atcaatactc cctatcgtca gaccgtagca gtcttgcgga
    12361 tcgtcctggc aatgactatc tccgacattc atcagggagt tgctgcgacc cagggtaact
    12421 tcaccgtttt caatctgcat ctcaccagta aagtcattgt tatctgcatt aagtaccaga
    12481 tcgccggaac ctgttttggt gattaacccg gtaccagcaa tagagtcaac agctccgtca
    12541 ttctctgtat tgccaataac cagcgttttt ccgtcggcaa tatcaaaggt aacttcactt
    12601 aagccgagat acataaagcc acccgccgca gtggaaggac catctccata gcctgctgcg
    12661 ctattgttct catcgactaa cacgccgccg ttctggctgt agctgtcatc aacagaaata
    12721 tcaataagat agggagccgt cgcgctattg gtatatatcg ccccgccata accttcggct
    12781 gtgttatttg taaaggcggt gttatttatt atcgtataac ctgaaggatg cttgctgtcg
    12841 ctattattat cggtaacatc gattgccccg ccatcgccat cactgtaact tgttgatgta
    12901 tatgcctggt tattgttaaa aacatcatca cttaaataga cgtcattatt aatggtataa
    12961 attgcgccac ctttgccatc attagcgata ttattgcgaa acatggcgtt agtgacacgt
    13021 aaatcgacgg caccagtatc attagtacca gaagaataga ttgcgccacc atagccgcct
    13081 gcgacgttac cggaaaaaat aacatcagta agattcagcg ttgagttttc tttagcaaat
    13141 atggccccgc cgttattata ttctcctgta acggtgttat tggcaaacag agtcattcca
    13201 gtttcatttt ctggtaatag tgaaaattcc gctccttgtt gcaaaaatac ggctcctccg
    13261 ctggcattat tggtcatatc cgaaaaaacc agccattgcc catcggcgac actccagtcc
    13321 tgcgtaatgc ctgaaaggct ttgcctgctg gcgtgacaac tcgctttgat atcatatccc
    13381 tggcataaat caatggccgc cgcgaaaccg ttagcagaga aaagagatgc aatcattgac
    13441 gggagtagag ataaatactc cttgcgtaga aagataatcc gcatattaat ctgaccatca
    13501 ttttatataa aacggcatat ttaaacgcat tatacaatat gggttatctt tcttttgtaa
    13561 attgtccaac ccgatgcaat ggctattttg ttattatttg atgaataata tcagtgcgtc
    13621 ataattcaag ttaa
//
LOCUS       DAJOSY010000111        13614 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJOSY010000111 DAJOSY010000000
VERSION     DAJOSY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.111"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(83..1129)
                     /gene="aroH"
                     /locus_tag="OHN27_003559"
     CDS             complement(83..1129)
                     /gene="aroH"
                     /locus_tag="OHN27_003559"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCQ2287935.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1286..2119)
                     /gene="ppsR"
                     /locus_tag="OHN27_003560"
     CDS             complement(1286..2119)
                     /gene="ppsR"
                     /locus_tag="OHN27_003560"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCQ2287936.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2452..4830
                     /gene="ppsA"
                     /locus_tag="OHN27_003561"
     CDS             2452..4830
                     /gene="ppsA"
                     /locus_tag="OHN27_003561"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCQ2287937.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4887..6533)
                     /gene="fadK"
                     /locus_tag="OHN27_003562"
     CDS             complement(4887..6533)
                     /gene="fadK"
                     /locus_tag="OHN27_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCQ2287938.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(6590..6883)
                     /locus_tag="OHN27_003563"
     CDS             complement(6590..6883)
                     /locus_tag="OHN27_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCQ2287939.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWKYPAGTFGIDFRYG"
     gene            complement(6880..8169)
                     /locus_tag="OHN27_003564"
     CDS             complement(6880..8169)
                     /locus_tag="OHN27_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCQ2287940.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEHAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILETNVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8225..9163)
                     /locus_tag="OHN27_003565"
     CDS             complement(8225..9163)
                     /locus_tag="OHN27_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCQ2287941.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDAAAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCASDNSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(9183..9947)
                     /locus_tag="OHN27_003566"
     CDS             complement(9183..9947)
                     /locus_tag="OHN27_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCQ2287942.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            10263..11174
                     /locus_tag="OHN27_003567"
     CDS             10263..11174
                     /locus_tag="OHN27_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCQ2287943.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWLLTNTELSQAEISWRVGYENVDHFGKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(11217..12368)
                     /locus_tag="OHN27_003568"
     CDS             complement(11217..12368)
                     /locus_tag="OHN27_003568"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCQ2287944.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(12382..>13614)
                     /locus_tag="OHN27_003569"
     CDS             complement(12382..>13614)
                     /locus_tag="OHN27_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HCQ2287945.1"
                     /translation="TQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKEDLIKL
                     VEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNNGGIVM
                     MQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLDDSTKL
                     SLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVETGPIGG
                     ITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKFNGKIM
                     GTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEITFSGK
                     IALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPELKLMD
                     ERLFIDAAMGFVLPEAAH"
BASE COUNT         3297 a         3446 c         3449 g         3422 t
ORIGIN      
        1 ggctgaccag agcaagcagt aatttgttga gaagacaaca agttgcgcag aaatgcccat
       61 tccgtcagga atgggcacgc attcagaagc gggtatctac cgcagaggcg agtttttcga
      121 ccaggcgttc ggtatcctcc cagcccagac acgggtcggt aatggattga ccgtaagtga
      181 gcggctgact gccgacgatt ttttgcgttc cttcgcgcag gaaactttcc gccataattc
      241 cagcaatcgc cgtagagcca ttgcggattt gctgacaaat atcctcacaa acttctaact
      301 ggcgacggtg ctgcttctgg cagttaccgt ggctgaaatc caccaccaga tgttcaggta
      361 aatcaaactc gtgcagcgta tcgcaggctg cggcgatatc atcggcatga taattcggtt
      421 ttttgccgcc acgcataata atgtggccat acgggttgcc gctggtctga tagatggtca
      481 tctgaccatt tttgtctggc gagaggaaca tatggctggc gcgggctgcg cggatagcat
      541 ccacagcaat ccgcgtattg ccatcggtac catttttaaa acctaccgga caggagagtg
      601 ccgaagccat ttcgcggtgg atctgacttt cggtagtacg tgcgccaatc gcgccccaac
      661 tgattaaatc agcaataaac tgaccggtca ccatatcgag gaactcggtc gcggttggga
      721 cgcccagctc atttacctgt aaaagtaatt tgcgcgccag ctccagaccg tgatttaccc
      781 gatagctgcc gtttaaatct ggatcggaga ttagtccttt ccagccgaca acagttcgtg
      841 gtttttcaaa ataggtgcgc attacgattt ccagccgtga ctggtactgg ttgcgcagcg
      901 actgcagacg ggtggcgtac tccattgcag cggtgagatc gtggatcgag caggggccaa
      961 taatgaccaa cagtcgctta tcttcaccat tcagtatttt ttcaattctg cggcgggagt
     1021 cggtgacatg ggtggcgacg ccaggcgtta cgggataccg tagcgcgagt tcggcgggcg
     1081 ttaccaggct ctcaatacgc gcagtacgga gttcgtcagt tctgttcatt acaagtctca
     1141 gaaatttgtt gcttctacgg caggactgcc gggaagtgat gcgcatcacg ataaaccaat
     1201 cgctgctgat ttcaagtgcg aggtgtgtca cacctcgccg ggtggttagc atgataacaa
     1261 aaaaataagc taatgcacta gttctctagt acattcggcg actaaggccc atgatatcga
     1321 ggatcttggt ggcaatctct tctaccgaat aattggtact gttaatccac gggatctgat
     1381 ttttacggta caaggcttcc acttccgcga cttccatcct gcactgacga agcgaggcat
     1441 agcgactgtt ctcccgacgt tcctcgcgaa tcgccgccag acgttccggg tcgatagtca
     1501 ggccgaacaa tttatgctga agcggtttga gcgacgcggg tagcaccaga ttatccatat
     1561 cgtcggcaat aaaggggtag tttgccgcgc ggataccaaa ctgcattgcc agatacagac
     1621 tggtgggggt tttaccacag cgagaaacac cgagcaggat cacctgagcc tggtccagat
     1681 tgcgcaacga aatgccgtca tcgtgggcga gggtgtaatc aatcgccgca atgcgcgcat
     1741 catatttatt gagattatta gggttaaggc catgggtacg atgagcaatc ggcgttggat
     1801 ccagtttcat ctcttgttgt agcggggcaa ccagcgcctg aacgatatcc tggcaaaagc
     1861 cttcactttg caagatgatg gcgcgaatct ccggcaacac gatggagtag aagaccagcg
     1921 ggcgcacgcc tgtctggtga taaattgcgt caatctgatc cttcactgcc cgtgcacggc
     1981 tctcattttc gacaaacggc agcgtgatgc tgctgatagt gacgggaaat tgtgacatta
     2041 ctgcgtgtcc taatacctcc gcagttattg ccgtaccatc agaaatataa aaaacgtggc
     2101 gatcaacagc attatccatt ttgttcttcc cgtgatgcag acataattct atgaaagcat
     2161 aaattaaaaa cgcacagaag cgtagaacgt tatgtctggt ttataaaatg aaccttcaat
     2221 tttatttttt atgaaaacag catttcattt ttatggtttc gtttataccg atggtttatg
     2281 tggaaattgt cgaagagagc agatttgcgc aacgctggga tcagtcttaa aaagtaaaaa
     2341 aatatatttg cttgaacgat tcaccgtttt tttcatccgg ttaaatatgc aaagataaat
     2401 gcgcagaaat gtgtttctca aaccgttcat ttatcacaaa aggattgttc gatgtccaac
     2461 aatggctcgt caccgctggt gctttggtat aaccaactcg gcatgaatga tgtagacagg
     2521 gttgggggca aaaatgcctc cctgggtgaa atgattacta atctttccgg aatgggtgtt
     2581 tccgttccga atggtttcgc cacaaccgcc gacgcgttta accagtttct ggaccaaagc
     2641 ggcgtaaacc agcgcattta tgaactgctg gataaaacgg atattgacga tgttactcag
     2701 cttgcgaaag cgggcgcgca aatccgccag tggattatcg acactccctt ccagcctgag
     2761 ctggaaaacg ccatccgcga agcctatgca cagctttccg ccgatgacga aaacgcctct
     2821 tttgcggtgc gctcctccgc caccgcagaa gatatgccgg acgcttcttt tgccggtcag
     2881 caggaaacct tcctcaacgt tcagggtttt gacgccgttc tcgtggcagt gaaacatgta
     2941 tttgcttctc tgtttaacga tcgcgccatc tcttatcgtg tgcaccaggg ttacgatcac
     3001 cgtggtgtgg cgctctccgc cggtgttcaa cggatggtgc gctctgacct cgcatcatct
     3061 ggcgtgatgt tctccattga taccgaatcc ggctttgacc aggtggtgtt tatcacttcc
     3121 gcatggggcc ttggtgagat ggtcgtgcag ggtgcggtta acccggatga gttttacgtg
     3181 cataaaccga cactggcggc gaatcgcccg gctatcgtgc gccgcaccat ggggtcgaaa
     3241 aaaatccgca tggtttacgc gccgacccag gagcacggca agcaggttaa aatcgaagac
     3301 gtaccgcagg aacagcgtga catcttctcg ctgaccaacg aagaagtgca ggaactggca
     3361 aaacaggccg tacaaattga gaaacactac ggtcgcccga tggatattga gtgggcgaaa
     3421 gatggccaca ccggtaaact gttcattgtg caggcgcgtc cggaaaccgt gcgctcacgc
     3481 ggtcaggtca tggagcgtta tacgctgcat tcacagggta agattatcgc cgaaggccgt
     3541 gctatcggtc atcgcatcgg tgcgggtccg gtgaaagtca tccatgatat cagcgaaatg
     3601 aaccgcatcg aacctggtga cgtgctggtc actgacatga ccgacccgga ctgggaaccg
     3661 atcatgaaga aagcatctgc catcgtcacc aaccgtggcg gtcgtacctg tcacgcggcg
     3721 atcatcgctc gtgaactggg cattccggcg gtagtgggct gtggtgatgc aacagaacgg
     3781 atgaaagacg gtgagaacgt cactgtttct tgtgccgaag gtgataccgg ttacgtctat
     3841 gcggagttgc tggaatttag cgtgaaaagc tccagcgtag aaacgatgcc ggatctgccg
     3901 ttgaaagtga tgatgaacgt cggtaacccg gaccgagctt tcgacttcgc ctgtctgccg
     3961 aacgaaggcg tgggacttgc gcgtctggaa tttatcatca accgtatgat tggcgtccac
     4021 ccacgcgcac tgcttgagtt tgacgatcag gaaccgcagt tgcaaaacga aatccgcgag
     4081 atgatgaaag gttttgattc tccgcgtgaa ttttacgttg gtcgtctgac tgaagggatc
     4141 gcgacgctgg gtgccgcgtt ttatccgaag cgcgtcattg tccgtctctc tgattttaaa
     4201 tcgaacgaat atgccaacct ggtcggtggt gagcgttacg agccagatga agagaacccg
     4261 atgctcggct tccgtggcgc gggacgctat gtttccgaca gcttccgcga ctgtttcgcg
     4321 ctggagtgcg aagcagtgaa acgtgtgcgc aacgacatgg ggctgaccaa cgttgagatc
     4381 atgatcccgt tcgtgcgtac cgtagatcag gcgaaagcgg tggttgagga actggcgcgt
     4441 caggggctga agcgtggtga gaacgggctg aaaatcatca tgatgtgtga aatcccgtcc
     4501 aacgccttgc tggccgagca gttcctcgaa tatttcgacg gcttctcaat tggctcaaac
     4561 gatatgacgc agctggcgct cggtctggac cgtgactccg gcgtggtgtc tgaattgttc
     4621 gatgagcgca acgatgcggt gaaagcactg ttgtcgatgg cgattcgtgc cgcgaagaaa
     4681 cagggcaaat atgtcgggat ttgcggtcag ggtccgtccg accacgaaga ctttgccgca
     4741 tggttgatgg aagaggggat cgatagcctg tctctgaatc cggacaccgt ggtgcaaacc
     4801 tggttaagcc tggctgaact gaagaaataa aataaatacc cggcggcgtt ttgtcgtcgg
     4861 gtttatgtga tccccgaaga tgaaacttat tcaatctctt cacagacatc ctgagttaac
     4921 cgccgcataa tatcttttct taacaaaaac ttttgtattt tacctgaggc agtgcgcggt
     4981 agtttttcga ttaccacgat atgttcagga tatttatatt ttgcgacccg tttacggcta
     5041 aaaaaagcca ccacctcttc cagcgataat gaatgatgcg gcgctttcag cacgacataa
     5101 gcgcatgatc gttcacctaa acgttcatcg ggcattgcaa ccacacaggc atcgtgaatt
     5161 ttaggatgct gcaataaaat atcttccact tcacggctgc taatattttc gccgccgcgg
     5221 acaataatat cttttttgcg tccggtaatt tttatatagc cagcctcatc catacggcag
     5281 agatcgccgc tgtaatacca gccttcttca tccagcgcac gggcggttaa ttcaggttca
     5341 tcaaaatatc ccataaacac attggggcca cgcgaggctt cttcaccttc gcaacctggc
     5401 ggtaaggtct tgcgtgcgtc atcgaccact ttaatctcta cacctgcggc agcgtaacca
     5461 tcggtgtgca taaagcgcga caaaggatca tcgagattca ccaccgcatg cggcgaactt
     5521 tctgtggaac cataaacact taataattta atgccgcgct gctggcattc acgtgccact
     5581 tttttgggga ttgtggtacc gccgcaaaga aagaagcgca gcgctgaaag gtctgcgggt
     5641 tgtttctcta gtaaattcaa aagatcatag acaaacggcg ttgcgccgag catacaggtg
     5701 caacgctgct gctcaagcag cgcgagacac gcatcaggag tgaaaatatc taacaacacg
     5761 ctgcgagcgc caatcaaaaa tggtgctgtt acgccatgca gaaagcccgt tgcgtggccg
     5821 agtggcgcag gcatcataaa gacatcctgc caggtcagat tcagccgcgc gcaataagcc
     5881 cgctcactgg cgagaatatt gttatgcgtt agcatcacgc cctttggcag accctcggtt
     5941 ccggaggtaa acagcaccgc agctaattca tcgccgtggg tcgttatcgc cgtggtcagt
     6001 ggggtattgt cggcgataat ctgacttaat gagagggaag aggtggcggg agccagtttg
     6061 tccacgccga caatttgttg tagttgtgga agctgatttt gcagcggcag gattaaatct
     6121 accggacgcg tttgtttaaa caacgtcggt gcaaagaaca tttttgcctg acacttatta
     6181 agcacccaca ccagttctgc ttcccgccag gaaggcaaca gcggtacgga aaccgcaccg
     6241 attttcaggc aggcaagata gataacggta aattcacacc agcctggcag ttgaaatgcg
     6301 atgcgatcgc ctgattcaat acccttcgct aacatccagt ttgccagaca gctcgcggcg
     6361 tgatcgagtg cgctataggt gtacgatgct ccatgattat cgaccacggc aattttgtct
     6421 ggcatcgcac gagcggtctg ctgccagtaa tcggccagcg aagcatcgcc ccatagccct
     6481 tgctgacgat acgccgcacg acgttgttcg ttaaacgtta atgtcacttt cattttggac
     6541 tctcgaaaca gaacatcagg cccgatgtgc gggcctgtgg gatgcgtgat tagccgtaac
     6601 ggaaatcaat accgaaggta cctgcgggat atttccactg ttcgagaata gtgttaccgc
     6661 acaacacccg acaggtgccg cactccagac aaccggcaga atcaaaatga atgttgccgg
     6721 cgtcatcctg cttataaagt ccggcagggc aggctttcat taatttacgg aattcattga
     6781 tatcgggatt tgccgccaaa atgatatgcg ggtggccctc atcaacatgg aatttattga
     6841 cgcctaattt gatgtcgacg ttaaccgtag cgttctggct catagcgcgg ttgctccctt
     6901 aatgccatct ttcagcaagt tgatcagccc gattttcttc gcatgtccca tgatcatttt
     6961 gcgtaccggc tggtttggtt tgccgtcaat ggtgaacatc tcgttcatga tgtcggctac
     7021 cattcgtggg tattggctaa acaggcgcgg gttttccatc agcgccggga tcttgcgaaa
     7081 atgctgcata tcacgcatga cgcagctttg ttccagctca cgtttgtatt gcgcaagact
     7141 gctggcggag aaatccgcgc gttctttggc ggcgatcacc gttgtggcgg cagcctgagc
     7201 cgatgcaatg gctaaatcca tgccgcggac cgtaaaaccc aaattcaggc agaagcctgc
     7261 ggcgtcacca acgatcatca cgccctcgtt aaccagttgc ggcaccattg ccagaccgcc
     7321 ttccggcacc atatgcgcgg aatattcaag cagtttgccg ccgctaatca gcgggcgaat
     7381 ggcggggtgt tgtttaaaat cttccagcat ttgcggcacg cttttttgcg catgggcgat
     7441 atcacccaat ccacaaacca gccccaacga tatggaatcc ttattggtat agaggaatcc
     7501 gccacccatc aggccgtcag aaggggaacc ggcaaacagc caggcggcac cttcctcgcc
     7561 cgtaatatta aagcgatcgt tgatctgttc tggtgtgagg ccaataacct ctttaacacc
     7621 aacagcgtaa tgatgcggat cggaagcggg aaccattccc agcgagcggc caagcatcga
     7681 gttaacgcca tcagccagaa tcaccacatt cgtttcgaga atatcatccc cagcctggac
     7741 gccagtgacc ttgtttcctt cacgaaccag cgcatcgacg cgaactcccg ggataaactg
     7801 tgcgcccgcc tgctcggcat gttccatcaa ccacgggtcc agacgattac gcaatacggt
     7861 ataagatgcg tgttgcggaa catctggttg ctcgcggtga aaatcgaggg taacggcgct
     7921 ctcttcggtt aagaaggaga ttttctcgcg tgtgaccttg cgttctaccg gcgctgatgc
     7981 tgcaaagcct ggaatgattg cttcaagtgt gtgggcataa aggcgcccgc cggtcatgtt
     8041 tttacatccg gcactgtcgc cgcgttctat caccaggaca tccagcccgg ctcgtgccat
     8101 gaccagtgcg gcaacgctac cagcaacgcc cgcaccgacc acaatggcat caaatttgtc
     8161 atccgacatg cgttatgctc ccttcccgct gcgtttacag cgggagatgt ggttgaaagg
     8221 ggaattagcg gctcaactgg ctaatcaggg cagggacgac tttgtagata tcgcccacca
     8281 gaccgtagtc ggcatagttg aagattggcg catttttatc tttattgatg gcgacaatca
     8341 cttttgcgcc gttgccgcca accatatgtt ggatctgccc ggagatcccc agcgtcaggt
     8401 agagatcgga tttcagcaac acgccggaga caccgatata acgttcacgc tccatccagt
     8461 tctcgccttc ggcaattgga cgtgaacagc cgacttcagc attcagcacc gccgccagtt
     8521 cgtggaccat tttcaggtca tcctgcgctg ccagaccacg accgacgcca accacacgtt
     8581 ttgctttgct caggtccacg ctgcttgccg ctttcgcacg gcgctcgcga cagagaattt
     8641 catgacgcgg agcaacataa ggtaccgtct ctgtcgggca ctgatgagag ttatcactgg
     8701 cgcacggttc ctgaacaccg ggtgcaaggg taatgatcgc cagcgagctg ttgatctttt
     8761 cctgagcgaa cgctaacccg ccatacatcc ggtgttcggc gcaaatgtga ccatcgacaa
     8821 tatccaccgc cgcggcatcg ttcaccagcg ccgcattcag ttgcacactt aaccgtgctg
     8881 ccagagcttt accacgtttc gtcgcggcca gtagcaacat agcggggtgt ttatctttca
     8941 gcagagcagc aatgctttcg gcgtaatttt cagtgcgttg cagcgcgtcg ttttgctcaa
     9001 gaacataaat acattttgga ccataaggca taactgcctg cgcctggtcg gtattttgta
     9061 caatggcata cacctgttgg ccccattgct gagcgccgcc aaacagttca gcataacgtt
     9121 caggattatc gctaaagacc cagacgctgt ttaattgact catcgtttct tctccgtagg
     9181 cttcagttca gggctttctt cagatgttcc gccagctcgg caatggcctc cggcgaatcg
     9241 ttatcgagaa tgatgtgctt acgttctgtt tgcggcggta cgcgaatgcc ggtcagttca
     9301 gcaagtggtg cgctctggct ccagccaata tcacttgcct gccactgatt taccggtttt
     9361 ttacccgcgc cgagaatggc tttcatcgaa ggaatgcgtg gcacgttaat atcggaggtg
     9421 acgcagagca cggctggaac agagagttca ataacttcaa cgtcatcttc aagcgtgcgt
     9481 tcaatcacca gtgtattgcc ctgacgctga atagcactca ctgcattaat caccggaagt
     9541 tgcagaattt ctccgaccag caagccaacc tgctgggcat aaaggtcgcc ggaaccttca
     9601 ccaaagatca gtaaatcgaa gccgatcttt tcaactgctg ccgccagcgc ttttgcggta
     9661 tcgagaggca gtgcatgctc aagttgtgca tcctgcacca tatacaggct gtgcggcccg
     9721 cgggatagca cgtctttgcg cactttcgag ttctgcaaca atgagccgcc aacggtcagc
     9781 gcggctatct catcgtcatc tgttgcaagc tggcttgcag cttcaatggc attgagatcg
     9841 aactggctga ttttggcgtc ggcattgtcg aaattcaggg tgtattctgg agtgacaaca
     9901 atgtcctgtt cttcaggcac cagcttaaag caggttatta ttttcatgat ttctcctgtg
     9961 agtcgcatac ggaaccatct ccgatggaag gtaaaaatgg cctgaaaata gtgtgcgata
    10021 gcggtgcggg gttttcgacg gagcaagctg ttttccttgc caacaatctt gcaaaaaaaa
    10081 tgcgatcgct aaaacaaaag tgcaatttgc ggcaatatag ttattccatg accgtacaat
    10141 cttgcattca gtgtaaataa tcttgtttag ctctcgctaa acgcggttgt ataaccgcaa
    10201 tacacgccgt attttatccg gcatatattg tttttgcttg ctcatggaat agggaggttg
    10261 tcatgtatca acgctgtttt gataatgcca gtgaaacgct gtttgtcgct ggtaaaacgc
    10321 cacggctttc acgttttgca tttagcgatg atccaaaatg ggagtctgga catcacgttc
    10381 atgacaatga aaccgagctg atttacgtca agaaaggggt ggcaaggttt accatcgatt
    10441 cttcgttata tgtcgcgcat gcagatgata tagtggtgat agaacgcggc aggctgcatg
    10501 cggtggcctc tgacgttaac gatccggcaa cgacgtgtac ctgtgcgctg tacggctttc
    10561 agtttcaggg ggctgaggaa aatcatctac tgcaaccgca ttcttgtccg gtaattgccg
    10621 cggggcaggg aaaagaagtc attaaaacct tatttaatga gctaagtgtg attttgccgc
    10681 aaagtaaaaa tagccaaaca tcttcgttat gggatgcatt tgcctataca ttagcaattc
    10741 tttactacga aaactttaaa aatgcttatc gttcggagca ggggtatatt aaaaaagatg
    10801 ttctgataaa agatattctt ttctatctga ataataatta tcgcgaaaaa atcactttag
    10861 aacagttatc gaaaaaattt cgtgccagcg tcagttatat ttgccatgaa tttaccaaag
    10921 agtatcgtat ttccccaatt aactatgtta ttcaacggcg tatgacggaa gcgaaatggt
    10981 tactcactaa tactgaatta tcacaggcag agatctcctg gcgtgtgggt tatgaaaatg
    11041 tcgatcactt tggcaaactg tttttgcgcc atgtcggctg ttcacccagc gattaccgca
    11101 ggcaatttaa aaactgtttt gcggaacaag aaatcctatc tgaatttcct caaccggtaa
    11161 gtcttgttgg ataaatctgg cggcgcagtt tgttaagaag ctgcgccgcc tgcagattat
    11221 ttgttctgat agtctttcag gatctgccga cctgctacgt aaatcataat ttcgtctgta
    11281 ccgccgccga tacgttcaca acggacatca cgccagaagc gggagacgcg cgcttcatcg
    11341 gtatagccca gaccgcccat gatttgaatc gcatcatcaa tgacttccat tgcggtacgt
    11401 gcgcaataca gttttgccag cgccgcgctg gtgcgcagtg actgatgctg atcggcttgc
    11461 catgccactt tcagcaccat gttgcgcatg ttgtcaatct taatcgccat cagcgccagt
    11521 ttttcctgga tcatctggtt atgaccaatg ggcttaccaa aagcgatacg ttggttggca
    11581 tagcgggcgg catcttcaaa ggcgcattcg gcaaagccgg tgctgcgcgc ggcgttgatc
    11641 aggcgctcca tctcaaagtt gtacatcaca ttgaggaaac ccattccttc ttcgcccacc
    11701 atgtcgctct cttcaacttc cacgttgtcg agatagactt cgcaggtgct gagcatatgc
    11761 cagccgattt tatgcagcgg gttaatctta atgccgggct tactggagtc gacccaccac
    11821 agggtgaagg cttttttggg atctttcggt tgcggatcgc gcgccaacac cagcatatac
    11881 gggtactctt tcgcaccggt aataaaggtt ttctgtccgt tgatgtaaac cttgccgttt
    11941 ttacgcgtgt aagtggtggt ggcactgttg ttatctgagc cagcacctgg ttccgtcaac
    12001 gccagggcat aggcggggtc gccggtttcc agggtgcttt ctgccgtttt acgtagctgc
    12061 tctgcagaac cgaaacggcg catactgtga atacattgac cgttggtaat taaaaatgcc
    12121 ggagcaccgc attttgacac ttccatcagc gccagcattt gggtgacgta atccgcaggg
    12181 ataccaccaa attcttccgg cacgccaagc atggaaatac cgttatccgc cagcgcccgc
    12241 ataaactcac gcggatatgt cccgttttga tcgcaggtgc ggaaatactc ttccggaaag
    12301 ttagtcgtaa tcagttcgcg aatactggcc agcagcagtt cttgttcttc agttaaagaa
    12361 aaatccatca ttatactcct gttaatgagc cgcttcaggc aggacaaaac ccatcgccgc
    12421 atcgataaat aatctttcgt ccatcagttt gagttctggc gaaatcactg gggtgaaatc
    12481 cattttgtcg agaatatctt tttgtaaatc gacgccaggg gcgatttcga ttaaatgcag
    12541 gccgtcttct ttcagcgtga atactgcgcg ctcagtgata taacgaacat ccagccctcg
    12601 ctcgagagcg atttttccgc tgaaagtaat ttccggtagt tcccgaataa atttcttcac
    12661 ccgtccttcc tggacgatat ttaatttgcc gtcggtaatt tctgttttta aactgcccgc
    12721 agttaatgtg ccgcagaaaa tgattttctt cgaagtggca ctgatatcaa taaatccacc
    12781 ggtgcccatg attttaccat tgaatttatg cacgccgacg ttaccgtgct ggtcgacttc
    12841 agcaaaactc aaataacaaa catccagacc gccaccgtga taaaaatcaa actgggacgt
    12901 catatccaga atggcacggg tattcacgtt cgcgccaaag gcgatcccct gtgaagtaat
    12961 tccgccaatc ggacctgttt ctaccgtcag aataaagtca tcagcacaac cttcttctcg
    13021 ggcgaccagg ccaatgccgt cagcaatacc gacgccgaca ttccccaccg cgcctttacg
    13081 catttcgaat aatgcgcgcc gcgcaactaa tttacgttgg tttaggggca ggctaagttt
    13141 ggtactgtca tcaagggtga agtcaccaga aataaagcgg ttaaccggtg caccgccata
    13201 cagttgggtt tgatccggat cgaccaccac aatatccacc agataacccg gaatacggac
    13261 agatttagga tgcagcgtgg ctttcttaac cattttctgc acctgcatca tcacaatacc
    13321 gccgttattg tgcaccgcct gggcaataac caatgcgtcg agatacatca cctcatcttc
    13381 aaaagtggcg tagccttcgc tgtcgcaggt ggtagcgcga atgaaggcga tatctggcgc
    13441 aatcgctttg taatagagat attctttgtt atcaaactcg accagtttaa tcaggtcttc
    13501 tttagtgact tcattcagtt tgccgccttg ctggcgtgga tcgacaaatg tcccgatgcc
    13561 aatatcacta ataataccag gctggtgggc tgcggcggcg cgtaaggttt gtgt
//
LOCUS       DAJOSY010000112        13484 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJOSY010000112 DAJOSY010000000
VERSION     DAJOSY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.112"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            2..1456
                     /locus_tag="OHN27_003570"
     CDS             2..1456
                     /locus_tag="OHN27_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCQ2287946.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKVTGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            1478..2887
                     /locus_tag="OHN27_003571"
     CDS             1478..2887
                     /locus_tag="OHN27_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2287947.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLCVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2865..3644
                     /locus_tag="OHN27_003572"
     CDS             2865..3644
                     /locus_tag="OHN27_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCQ2287948.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3641..4501
                     /locus_tag="OHN27_003573"
     CDS             3641..4501
                     /locus_tag="OHN27_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCQ2287949.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTGDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVDVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(4649..5224)
                     /locus_tag="OHN27_003574"
     CDS             complement(4649..5224)
                     /locus_tag="OHN27_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCQ2287950.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(5241..5501)
                     /locus_tag="OHN27_003575"
     CDS             complement(5241..5501)
                     /locus_tag="OHN27_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCQ2287951.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLINACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(5492..6763)
                     /locus_tag="OHN27_003576"
     CDS             complement(5492..6763)
                     /locus_tag="OHN27_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCQ2287952.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLAGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6841..7203)
                     /gene="queD"
                     /locus_tag="OHN27_003577"
     CDS             complement(6841..7203)
                     /gene="queD"
                     /locus_tag="OHN27_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCQ2287953.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            7522..9321
                     /gene="cysJ"
                     /locus_tag="OHN27_003578"
     CDS             7522..9321
                     /gene="cysJ"
                     /locus_tag="OHN27_003578"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCQ2287954.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            9321..11033
                     /gene="cysI"
                     /locus_tag="OHN27_003579"
     CDS             9321..11033
                     /gene="cysI"
                     /locus_tag="OHN27_003579"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCQ2287955.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            11107..11841
                     /gene="cysH"
                     /locus_tag="OHN27_003580"
     CDS             11107..11841
                     /gene="cysH"
                     /locus_tag="OHN27_003580"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCQ2287956.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            12200..>13484
                     /gene="cas3"
                     /locus_tag="OHN27_003581"
     CDS             12200..>13484
                     /gene="cas3"
                     /locus_tag="OHN27_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCQ2287957.1"
                     /translation="MEPFKYICHYWGKSSKSLTKGNDIHLLIYHCLDVAAVADCWWDQ
                     SVVLQNTFCRNEMLSKQRVKAWLLFFIALHDIGKFDIRFQYKSAESWLKLNPATPSLN
                     GPSTQMCRKFNHGAAGLYWFNQDSLSEQSLGDFFSFFDAAPHPYESWFPWVEAVTGHH
                     GFILHSQDQDKSRWEMPASLASYAAQDKQAREEWISVLEALFLTPAGLSINDIPPDCS
                     SLLAGFCSLADWLGSWTTTNTFLFNEDAPSDINALRTYFQDRQQDASRVLELSGLVSN
                     KRCYEGVHALLDNGYQPRQLQVLVDALPVAPGLTVIEAPTGSGKTETALAYAWKLIDQ
                     QIADSVIFALPTQATANAMLTRMEASASHLFSSPNLILAHGNSRFNHLFQSIKSRAIT
                     EQGQEEAWVQCCQWLSQSNKKVFLGQIGVCTIDQVL"
BASE COUNT         3283 a         3394 c         3653 g         3154 t
ORIGIN      
        1 tatgtcttta tctcgcgcag cgattgtcga ccagctaaag gaaattgttg gtgcagatcg
       61 cgtaattacc gatgaaacag tattaaagaa aaacagtatt gaccgttttc gtaaatttcc
      121 ggatattcat ggcatttata ctttgccgat tccggcagcg gtcgtaaaac tcggttccac
      181 agagcaagta tcccgtgtgc tgaattttat gaatgcgcac aaaattaacg gtgtgccgcg
      241 taccggtgct tccgccaccg aaggtgggct ggaaactgtt gtagaaaact cggtggtgct
      301 cgacggctcc gccatgaatc aaatcattaa tattgatatt gagaatatgc aggcgacggc
      361 gcaatgtggt gttccgctgg aagtgctgga aaacgcgttg cgtgaaaaag gttacaccac
      421 ggggcattct ccgcagtcaa agccgctggc gcagatgggc ggcctggtag caacccgaag
      481 tatcgggcag ttctccacac tctacggcgc aatcgaagat atggtcgttg gtctggaagc
      541 agtattggct gatggcaccg tcacacgcat taaaaacgtg ccacgccgcg cggctggccc
      601 ggacattcgt catatcatca tcggcaacga aggtgcattg tgctatatca ctgaagtaac
      661 agtgaaaatc tttaaattca ccccggaaaa caacctcttc tacggctata tcctggaaga
      721 catgaaaacc ggcttcaaca tcctgcgtga aatcatggtg gaagggtatc gtccgtcaat
      781 cgctcgtttg tatgacgctg aagatggcac ccaacacttc acccattttg ccgacggaaa
      841 atgcgtgctg atctttatgg ctgaaggtaa ccctcgcatt gcgaaggtga cgggcgaagg
      901 gattgcggaa atcgttgccc gctacccgca atgccagcgc gtggacagca agctgatcga
      961 aacctggttt aacaacctga actggggacc ggataaagtg gctgccgaac gtgtgcagat
     1021 cctcaaaacc ggcaacatgg gctttaccac cgaagtatcc ggctgctgga gctgcatcca
     1081 cgaaatctac gaaagcgtta ttaaccgtat tcgtactgag ttcccgcacg ccgacgacat
     1141 caccatgctg ggcggtcatt cctctcatag ctatcagaac ggcaccaaca tgtacttcgt
     1201 ctacgattac aacgtcgttg actgtaagcc ggaagaggaa atcgacaagt accacaatcc
     1261 gctcaacaag atcatctgtg aagaaaccat tcgcctcggc ggttcgatgg tgcaccacca
     1321 cggtatcggt aaacatcgcg ttcactggag caaactggaa cacggcagcg cgtgggcgtt
     1381 gctggaaggg ctgaaaaagc agttcgatcc taatggcatt atgaatacgg gtactatcta
     1441 tccgattgaa aaataatgtg tcaggcagcg ttccgcgatg acggggcgtt gccttttcgg
     1501 cttctcaggc gagaagccgt tcttattacc ggacaatgaa ggggtaaaga tgaacacttc
     1561 accggtgcga atggatgatt taccgcttaa ccgttttcac tgccgcattg ctgcgctcac
     1621 tttcggcgca cacctgaccg acggttatgt tctctgcgtc attggttacg ccattattca
     1681 gcttacgccc gccatgcaac tgacgccgtt tatggcggga atgatcggcg gctcggcgct
     1741 ccttggtttg ttccttggca gcctggttct tgggtggatc tccgaccata ttggtcggca
     1801 aaaaatcttc accttcagct ttttgctgat tacgcttgct tcgtttttac aattttttgc
     1861 caccacgcca gagcatctta ttggactgcg cattttgatt ggcattggtc tgggaggcga
     1921 ttattcagta ggtcacacct tgctggctga attttccccg cgccgccatc gcggtatttt
     1981 gctgggcgca ttcagcgtgg tgtggaccgt aggctatgtg ctggcaagta ttgccggaca
     2041 tcactttatt tccgaaaacc cggaggcctg gcgctggcta ctggcatcgg cagctctgcc
     2101 cgcgttgttg attacgttat tacgttgggg aacgccagaa tcaccacgct ggctactgcg
     2161 ccaggggcgt tttgcagaag ctcacgctat cgtgcatcgc tattttggtc cccatgtttt
     2221 actgggcgat gaagtggtaa cggcgaccca taaacacatc aaaaccttgt tctcttcgcg
     2281 ttactggcgg cgcacggcgt ttaacagcgt cttctttgtc tgcctcgtaa tcccatggtt
     2341 tgtgatttat acctggctgc caactatcgc ccagactatt ggtctggaag atgcgctgac
     2401 tgccagcctg atgcttaatg cgttgttaat tgtgggcgcg ctgctgggat tagttctgac
     2461 gcacctgctg gcacatcgca aatttttgct gggaagtttt ttgctgctgg cggcaacgct
     2521 ggtagtcatg gcctgtttgc cttccggcag ttcattaacg ctgctgcttt ttgttctctt
     2581 cagcaccacc atttcggcag tcagtaatct ggtgggcatt ttgcctgcgg aaagttttcc
     2641 tactgacatt cgctcgctgg gcgtcggttt tgccactgcc atgagtcgac ttggcgcggc
     2701 ggtaagtact ggcctgctgc cgtgggtgct ggcgcagtgg ggaatgcaag tcaccttatt
     2761 gctcctggcg acagtgttgt tggttggttt tgttgtgacc tggctatggg caccagaaac
     2821 taaagccctc ccgctggtgg cggcgggaaa tgtaggaggt gcgaatgaac attctgttag
     2881 cgtttaaagc cgaaccggat gccggaatgc tggcggaaaa agagtggcag gcggcggctc
     2941 agggtaaaag cggaccggat atttcgctac tgcgaagttt actcggtgct gatgaacagg
     3001 ccgctgccgc gctgctgttg gcgcagagaa aaaacggtac gccgatgtct ttaaccgcgt
     3061 tgagcatggg ggatgaacgg gcattgcact ggctgcgcta tctcatggct cttgggtttg
     3121 aggaagctgt attgctggag acggcggcag atctgcgctt tgcgccggaa tttgtcgctc
     3181 gccatattgc cgaatggcag catcagaatc cgctggatct gataattacc ggctgccaaa
     3241 gcagcgaagg gcagaatggg caaacgccat ttttgctggc ggaaatgctg ggctggccct
     3301 gcttcaccca ggtggagcgt ttcactctcg acgcgctgtt tatcaccctc gaacagcgta
     3361 ctgaacatgg gttgcgttgt tgccgggttc gcttgcctgc ggtgattgcc gtgcgtcagt
     3421 gcggtgaagt ggccttgcca gtaccgggaa tgcgccagcg aatggctgcg gggaaagcag
     3481 aaattatccg taaaactgtt gctgccgaaa tgccagcgat gcagtgtctg caactggcaa
     3541 gagcagagca acgacgcgga gcgacgctga ttgatgggca aacggtagca gaaaaagctc
     3601 aaaagctatg gcaggactat ttacgccaaa ggatgcagcc atgaatatcg caattgtgac
     3661 aataaatcag gagaatgccg caatagcaag ctggctggcg gcgcaggatt tcagcggttg
     3721 taccctggcg cactggcaga ttgaacctca gcctgtagtg gcggaacagg tgctggatgc
     3781 gttggtggaa cagtggcagc gaacgcctgc ggacgttgtt ctctttccgc cgggaacgtt
     3841 tggcgatgaa ctgtcgaccc ggctggcctg gcgtttacat ggtgcaagca tctgccaggt
     3901 gacttcgtta gatattccaa ccgtaagcgt gcgcaaatcc cactggggaa atgcgctaac
     3961 ggcgacgctg caaacagaaa aacgcccgct atgcctttcc ctggcgcgtc aggcgggagc
     4021 agcgaaaaac gccacgttgc cctctggaat gcaacaactg aacattgtcc cgggtgcgct
     4081 tccggactgg ctggttagca caggggatct gaaaaatgtt acccgcgacc cattagctga
     4141 ggccagacgg gtgctggtgg tggggcaggg cggagaggca gataaccagg aaatcgctat
     4201 gctggcagaa aagctgggag ccgaagtggg ctacagtcgg gcgcgagtga tgaacggtgg
     4261 cgttgatgct gaaaaagtga ttggtatttc cggacatctg ctggcacctg aggtgtgtat
     4321 tgttgttggc gcttctggcg cagcagcgtt aatggcgggc gtacgcaata gcaagtttgt
     4381 ggtggcgatt aatcatgacg ccagcgctgc ggtattttca caggcagatg ttgacgtggt
     4441 tgatgactgg aaagtggtgc tggaggcact ggtgacaaac attcacgcag attgccaata
     4501 acgtgttgcc ggatgatcct gcctacggtt ctggcctggg gacttgtagg cctgataaga
     4561 cgcgtcaagc gtcgcatcag gcgctgaatg tcggatgtgg cgtgcggcct acaaatcaaa
     4621 ccaaattcaa ttggttacga ttatcccgtc aaagtaattt tttcgctaac gtccagaccc
     4681 cggtattcgt ggtggaaagc gccacgacac ccgcgttaat cgcattacgc gcatcttctt
     4741 catcgcacac cagcccaccg gcaatcagcg gttggcggat tttctctgtc acccagccca
     4801 gcactttggg catacagcct ggcaggatct caatacaatc cggattcgat tgcgcaactt
     4861 gcttatcaat gttgtgaaac gaaattgaat caacaataaa caggcgatga atacagaaaa
     4921 aaccttccgc tcttgccgct ttcaacattg aggctttggt actgataatg ccgtccgcct
     4981 cggtcaccag tttcaaaaac tgaataacga cttctttatt tgacgcgcct tccagcaaat
     5041 caacatgaat aaaagcatat ttcccggcgt tcttaatctt tttaacgata ttactgatgg
     5101 tgcagatatt gccgtacaac acggaaataa attggcattc agaatcgatt gccagttgca
     5161 ggctggcatt atctttaacg gcagcaatca ccggattctg gcggagcagg tgtaagaggg
     5221 gcataaatta tccttattat ttatccaaag cggtaggtga tgccgaatcc ggaaggcgga
     5281 tagcgccact gttgtagtgt tgattcgtcg cacagcaaac ggcaggtgcc acactccagg
     5341 caactgcgat agtcaatacg taagtcacct tccggtgtga gcgaaaaaag acctgccgga
     5401 caggcgttaa tcaaccgttc tgccgtctgg cgctcaacgg agtcagccgg aacaatgtgc
     5461 ggcgcatcag caacgcgcca gagattacgg gctacagaca tcgcagactc ctgataacat
     5521 cgcccgccag atgccacagg ccgtgacgac gtaaatgatg ccagaataac tggcgcagcg
     5581 gtggaacagg tttatcaccc tgatcccata aatcgcggga aatatcttgc attaacgcag
     5641 gccacgtacg gtaccatccc gggcgttgca aaagcgccgg aacatgctga taacgctgta
     5701 gaacatccca cagcaggctg cgctctacgt tgtgatgata aagcggaaac agattttgcg
     5761 gctcgcggtg ctggcaggcg cttatcagcg tttgtgccgc cgcctgcgcg ccagtcagcg
     5821 ccatatccat gccgcgcacg gaaattccgg tattgacgca actgcgcaac gcatcgccca
     5881 ccagcagcca gccgttaccg gcgtattgca ccggcatact gtgcaagcca ccttctggca
     5941 ccagatgcgc accatactcc agtgattccg tgtttttgat aagcgggcgc actgccggat
     6001 gcgctttaaa gcgagtcagc agctcgcttg ccggaacacg gctttgcgta agggaagaga
     6061 gcgggcaaac aatccctaac gagagcgttt gttgattagt ataaagaaat gcgccgccgg
     6121 ctaagtcatc acagatcctg ccgctgaaca gcaacgctgc gccttcgtta ttctccagat
     6181 gaaaacgttc ttcaatagcg gatggttcca gcgacagcac ttctttgatc cccaacgcca
     6241 tcgcttcacc agcaggacga gtcactaacc cgtgacgctc agccaggacg ctgttggcac
     6301 cttctgccag caccacataa cgggcacgga gaatatcgtc accacaaatg acaccacaca
     6361 ccctgccgtt ttcttcatac agtgcatcca ccgtcgctcc ggggatgcat tcgacacctt
     6421 cttcttcggc ttcggcaacc agccacggat cgaaccgtgc acgtaataca ctccaggatt
     6481 caccgccggg ctgtaagctg gaaaacgtcg ttgcgccatc cggcgttaac agggaaaggc
     6541 tttcgtgagt gatgcgtcgt tcaagaggcg cggtcagatg aaattgcggg aggagttccg
     6601 cgagtgcatg ggtatataac cgcccgccgg aaagattttt gctgccgggg atttcagcgc
     6661 gttccagtaa caaaacggat aaacccgctc gcgcgcagcg taacgcgcaa gcggtgcctg
     6721 caataccggc accaataata ataatgtcgc agtcgtcttc catgtcatgc tccagatatc
     6781 cagaacacaa caccctaaca tagcgttact taagggaaat tgaccgccga cacactctta
     6841 tcattcgccg cgatagatac aacctgcggt gcaggtttct tttaccatca ccgcacttaa
     6901 cagcggcaca acgggtttaa cctgatccca aatccatttt gctaaaacct cgctggttgg
     6961 gttttccaga cctggaatat cattgagata atggtgatcg aggcgctcgt aggttggttt
     7021 aaacgccgct tttagttcag cgaaatcgat aatccagccc gtatgcggat cgacttcccc
     7081 ggtaatttcc agtcgcacca taaaggaatg cccgtgcagg cgaccacatt tatgcccttc
     7141 cgggacgtgt ggtaagcggt gagcggcttc gaaggtgaaa tctttaaata acgtggtgga
     7201 catcataatt tctctacagg acgataaaac cgccgtagag taccggaaag gctgtttttt
     7261 cgcattatct aaaacgactt tgttgcgcaa aatcgctgat ttatcttaat gattggctaa
     7321 attcatttgt ttttcattag gttggttaat ctattttgtt gttaaagact attgctaaaa
     7381 caggttagtc gatttggtta ttagttatcg ctatcccgtc tttaatccac accgtttgcc
     7441 ccgttaacct taccttctct tctgttttat gggcgctgac agggcgcaga aacagctttg
     7501 cttactggaa cataacgacg catgacgaca caggtcccac cttccgcgtt gcttccgttg
     7561 aacccggagc aactggcacg ccttcaggcg gccacgaccg atttaactcc cacccagctt
     7621 gcctgggttt ctggctattt ctggggcgta ctcaatcagc agcctgctgc gcttgcagcg
     7681 acgccagcgc cagccgcaga aatgccgggt ataactatta tctccgcctc gcaaaccggc
     7741 aatgcgcgcc gggttgctga agcattacgt gatgatttat tagcagcaaa actgaacgtt
     7801 aagctggtga acgcgggcga ctataaattc aaacaaatcg ccagcgaaaa actgctcatc
     7861 gtagtgacgt caacgcaagg ggaaggggaa ccgccggaag aagccgtcgc gctgcataag
     7921 ttcctgttct ccaaaaaagc gccaaagctg gaaaacaccg cgtttgccgt gtttagcctc
     7981 ggcgatagct cttatgaatt tttctgccag tccgggaaag atttcgacag caagctggcg
     8041 gaactgggtg gtgaacgcct gctcgaccgt gtcgatgccg atgttgaata ccaggctgct
     8101 gccagcgagt ggcgcgcccg cgtggttgat gcgcttaaat cgcgtgcgcc tgtcgcggca
     8161 ccttcgcaat ccgtcgctac tggcgcggta aatgaaatcc acaccagccc gtacagcaaa
     8221 gacgcgccgc tggtggctag cctctctgtt aaccagaaaa ttaccgggcg taactctgaa
     8281 aaagacgttc gccatatcga aattgactta ggtgactcgg gcctgcgtta ccagccgggt
     8341 gacgcgctgg gcgtctggta tcagaacgat ccggcactgg tgaaagaact tgtcgaactg
     8401 ctgtggctga aaggcgatga acctgtcacc gtcgagggca aaacgttgcc tctgaacgaa
     8461 gcgctacagt ggcacttcga actgaccgtc aacaccgcca acattgttga gaattacgcc
     8521 acgcttaccc gcagtgaaac actgctgccg ctggtgggcg ataaagcgaa gttacagcat
     8581 tacgccgcga cgacgccgat tgttgacatg gtgcgtttct ccccggcaca gcttgatgcc
     8641 gaagcgctaa ttaatctgct gcgcccgctg acgccgcgtc tctattccat tgcctcctcg
     8701 caggcggaag tcgagaacga agtacacgtc accgttggtg tggtgcgtta cgacgtggaa
     8761 ggccgcgccc gtgccggtgg tgcctccagc ttcctcgctg accgcgtgga agaagagggc
     8821 gaagtccgcg tatttatcga acataacgat aacttccgcc tgccagccaa tccagaaacc
     8881 ccggtgatta tgattggccc aggcaccggc atcgcgccgt tccgcgcctt tatgcagcaa
     8941 cgtgctgccg atgaagcgcc gggaaaaaac tggctgttct ttggcaaccc gcactttacg
     9001 gaagatttcc tctaccaggt ggagtggcag cgctacgtca aagagggtgt gctgacgcgg
     9061 atcgatctcg cctggtcgcg cgaccaaaaa gaaaaagttt acgtacaaga caaactgcgc
     9121 gaacagggcg cggagctgtg gcgctggatc aatgacggtg cccacattta tgtctgcggt
     9181 gacgctaatc gcatggcgaa agacgttgag caggcacttc tggaagtgat tgccgaattt
     9241 ggtggcatgg acaccgaagc ggcggatgaa tttttaagtg agctgcgcgt agagcgccgt
     9301 tatcagcgag atgtctacta atgagcgaaa aacatccagg gcctttagtg gtcgaaggaa
     9361 aactgacaga cgccgagcgc atgaagcttg aaagcaacta cctgcgcggc accattgcgg
     9421 aagatttaaa cgacggtctg accggcggct ttaagggcga caacttcctg ctgatccgct
     9481 tccacggcat gtatcagcag gatgaccgcg acatccgcgc cgaacgtgct gaacagaagc
     9541 tggagccgcg ccacgcgatg ctgttgcgct gccgtctgcc gggtggggtg attaccacta
     9601 aacagtggca ggcgatagac aaatttgccg gtgaaaacac catctatggc agcattcgcc
     9661 tgaccaaccg ccagacgttt cagttccacg gcattctgaa aaagaacgtc aaaccggtgc
     9721 accagatgct gcactcggtt ggtcttgatg cgctggcgac cgctaacgac atgaaccgta
     9781 acgtactctg cacctcgaac ccttacgagt cgcagctaca cgcggaagcg tatgaatggg
     9841 cgaagaaaat ctctgaacat ctgctgccgc gtacccgcgc gtatgcggag atctggctcg
     9901 atcaggaaaa agtcgccacc actgatgaag aaccgatcct cggccagacc tacctgccgc
     9961 gtaaattcaa aaccacggta gtgatcccgc cacagaacga tatcgatctg cacgccaacg
    10021 acatgaactt cgtggcgatc gccgaaaacg gcaagctggt gggctttaac ctgttggtgg
    10081 gcggtgggct ttccatcgaa cacggcaaca agaaaaccta cgcccgcacc gcgagtgagt
    10141 ttggctatct gccgctagag catacgctag cggtggcgga agccgtcgtg acaactcagc
    10201 gtgactgggg taaccgaacc gatcgtaaaa atgccaaaac caaatacacg ctggagcgcg
    10261 tgggggttga gacgtttaaa gcggaagtgg agcgtcgcgc ggggatcaaa tttgaaccga
    10321 tccgtccgta tgagttcacc ggacgcggag atcgtattgg ctgggttaag ggcattgatg
    10381 ataactggca cctgacgctg tttatcgaaa atggtcgcat ccttgattat ccggggcgtc
    10441 cgctgaaaac cggcctgctg gagatcgcga agatccacaa aggtgatttc cgcattacgg
    10501 cgaaccagaa tctgatcatc gccggtgtgc cggaaagcga gaaagcgaag atcgagaaga
    10561 tcgctaaaga gagcgggtta atgaatgccg tcacgccgca gcgtgaaaac tcaatggcct
    10621 gcgtgtcatt cccgacttgc ccgctggcga tggcggaagc ggagcgtttc ctgccgtctt
    10681 ttatcgacaa catcgataat ttaatggcga aacatggtgt cagcgatgag catatcgtga
    10741 tgcgtgtaac aggctgcccg aacggttgtg gtcgcgcgat gctggcggaa gtgggcctgg
    10801 tgggtaaagc gccgggtcgc tacaacctgc atcttggcgg caaccgcatt gggacacgta
    10861 tcccacggat gtataaagaa aacatcaccg agccggaaat cctggcgtcg cttgatgaac
    10921 tgatagggcg ctgggcgaaa gagcgcgaag cgggtgaagg cttcggcgac tttacggtgc
    10981 gtgcgggcat cattcgcccg gtgctcgatc cggcgcgcga tttgtgggat taaccatcag
    11041 cccggtcttg taggcctgat aagacgcgtg agcgtcgcat caggcaaggc aaacagtgag
    11101 gaatctatgt ccaaactcga tctaaacgcc ctgaacgaac tgccgaaggt agatcgcatt
    11161 ctggcgctgg cggaaactaa cgcccaactg gaaaaactgg acgctgaagg ccgcgtagcc
    11221 tgggcgctgg ataatctgcc cggtgaatat gtgctttctt ccagcttcgg cattcaggcg
    11281 gcggtgagcc tgcatctggt gaatcagatt cgcccggata ttccagtgat cctcaccgat
    11341 accggttact tgttcccgga aacctaccgc tttattgacg agttaacgga caaactcaag
    11401 ctcaacctga aagtgtaccg cgctaccgaa agcgcggcct ggcaggaagc acgctacggc
    11461 aaactgtggg aacagggcgt tgaaggcatt gaaaaataca atgacatcaa caaagtcgaa
    11521 ccgatgaacc gggcgctgaa agaactgaat gcgcaaacgt ggtttgctgg cctgcgccgt
    11581 gaacaatccg gcagtcgcgc caatttaccg gtgctggcaa ttcagcgtgg cgtatttaaa
    11641 gtgctgccga ttatcgactg ggataaccga actatttatc agtacctgca aaaacatggc
    11701 ctgaaatatc acccattatg ggacgaagga tatttatcgg tgggcgatac ccatacaacc
    11761 cgtaaatggg aacccggcat ggcggaagaa gaaacgcgtt tctttggctt aaaaagggaa
    11821 tgtgggttac acgaagggta aatattgccg gacaaattaa acgcctctgt cagaaatgat
    11881 gggggcgttt ttattttgcg agcagcattc ctgattttaa acgtgaacgc cgcgggaaga
    11941 attatcaggt atagaatggc gtcgggtgct tgaggctgtc tggcgttaag cattcgcgag
    12001 gttccagatg gacaaaagcc ccaggcgatt tttctatcaa cctgaggctt acgttcgaac
    12061 ccaaacaatt cgaatgttag tctcttcttt gcagggaggc aagacatgtg tatatcactg
    12121 taattcgata tttatgagca gcatcgaaaa atagcccgct gatatcatcg ataatactaa
    12181 aaaaacaggg aggctattaa tggaaccttt taaatatata tgccattact ggggaaaatc
    12241 ctcaaaaagc ttgacgaaag gaaatgatat tcatctgtta atttatcatt gccttgatgt
    12301 tgctgctgtt gcagattgct ggtgggatca atcagtcgta ctgcaaaata ctttttgccg
    12361 aaatgaaatg ctatcaaaac agagggtgaa ggcctggctg ttatttttca ttgctcttca
    12421 tgatattgga aagtttgata tacgattcca atataaatca gcagaaagtt ggctgaaatt
    12481 aaatcctgca acgccatcac ttaatggtcc atcaacacaa atgtgccgta aatttaatca
    12541 tggtgcagcc ggtctgtatt ggtttaacca ggattcactt tcagagcaat ctctcgggga
    12601 ttttttcagt ttttttgatg ccgctcctca tccttatgag tcctggtttc catgggtaga
    12661 ggccgttaca ggacatcatg gttttatatt acattcccag gatcaagata agtcgcgttg
    12721 ggaaatgcca gcttctctgg catcttatgc tgcgcaagat aaacaggctc gtgaggagtg
    12781 gatatctgta ctggaagcat tatttttaac gccagcgggg ttatctataa acgatatacc
    12841 acctgattgt tcatcactgt tagcaggttt ttgctcgctt gctgactggt taggctcctg
    12901 gactacaacg aatacctttc tgtttaatga ggatgcgcct tccgacataa atgctctgag
    12961 aacgtatttc caggaccgac agcaggatgc gagccgggta ttggagttga gtggacttgt
    13021 atcaaataag cgatgttatg aaggtgttca tgcactactg gacaatggct atcaacccag
    13081 acaattacag gtgttagttg atgctcttcc agtagctccc gggctgacgg taatagaggc
    13141 acctacaggc tccggtaaaa cggaaacagc gctggcctat gcttggaaac ttattgatca
    13201 acaaattgcg gatagtgtta tttttgccct cccaacacaa gctaccgcga atgctatgct
    13261 tacgagaatg gaagcgagcg cgagccactt attttcatcc ccaaatctta ttcttgctca
    13321 tggcaattca cggtttaacc acctctttca atcaataaaa tcacgcgcga ttactgaaca
    13381 ggggcaagaa gaagcgtggg ttcagtgttg tcagtggttg tcacaaagca ataagaaagt
    13441 gtttcttggg caaatcggcg tttgcacgat tgatcaggtg ttga
//
LOCUS       DAJOSY010000113        13206 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJOSY010000113 DAJOSY010000000
VERSION     DAJOSY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.113"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            163..1662
                     /gene="pitB"
                     /locus_tag="OHN27_003582"
     CDS             163..1662
                     /gene="pitB"
                     /locus_tag="OHN27_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCQ2287958.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLTV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1711..2403)
                     /locus_tag="OHN27_003583"
     CDS             complement(1711..2403)
                     /locus_tag="OHN27_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCQ2287959.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDVEK
                     AITDFMTARGYH"
     gene            2577..3290
                     /locus_tag="OHN27_003584"
     CDS             2577..3290
                     /locus_tag="OHN27_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCQ2287960.1"
                     /translation="MNQRNMSIINSTPVHVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAMHAALS"
     gene            3322..4080
                     /locus_tag="OHN27_003585"
     CDS             3322..4080
                     /locus_tag="OHN27_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287961.1"
                     /translation="MDALQTQTVISTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERCLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4126..5475
                     /locus_tag="OHN27_003586"
     CDS             4126..5475
                     /locus_tag="OHN27_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCQ2287962.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5475..6299
                     /locus_tag="OHN27_003587"
     CDS             5475..6299
                     /locus_tag="OHN27_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2287963.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHALKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            6311..6871
                     /locus_tag="OHN27_003588"
     CDS             6311..6871
                     /locus_tag="OHN27_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCQ2287964.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6902..7972
                     /gene="lptF"
                     /locus_tag="OHN27_003589"
     CDS             6902..7972
                     /gene="lptF"
                     /locus_tag="OHN27_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCQ2287965.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSSQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFTLLQKFSR"
     gene            7969..9048
                     /gene="lptG"
                     /locus_tag="OHN27_003590"
     CDS             7969..9048
                     /gene="lptG"
                     /locus_tag="OHN27_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCQ2287966.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9084..10256)
                     /locus_tag="OHN27_003591"
     CDS             complement(9084..10256)
                     /locus_tag="OHN27_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCQ2287967.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10256..10504)
                     /locus_tag="OHN27_003592"
     CDS             complement(10256..10504)
                     /locus_tag="OHN27_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCQ2287968.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10536..11450)
                     /locus_tag="OHN27_003593"
     CDS             complement(10536..11450)
                     /locus_tag="OHN27_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCQ2287969.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11447..13177)
                     /locus_tag="OHN27_003594"
     CDS             complement(11447..13177)
                     /locus_tag="OHN27_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCQ2287970.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENVLAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
BASE COUNT         3169 a         3262 c         3430 g         3345 t
ORIGIN      
        1 gtgctttgat tatttctaaa ggttttgaat gaatgcttat tgtctgatac acaagaaata
       61 acactctttt tatcgttaaa aaatgatatt tcactttgcc catgccgtta aagtcactga
      121 taatgcgtcc gttcgtaaat tcaaaatggc gtaatctaat atatgctaaa tttatttgtt
      181 ggccttgata tatacacagg gcttttgtta ttgcttgctc tggcatttgt gttgttctac
      241 gaagcaatca atggttttca tgacacggcg aatgcggtgg caaccgttat ttatactcgt
      301 gccatgcaac cacaacttgc tgtggtgatg gcggcatttt ttaacttttt tggcgtgtta
      361 ttgggcggac ttagcgttgc ctatgccatt gtccatatgt tgccaaccga tttgttgctg
      421 aatatggggt caacccacgg cctggcgatg gtcttttcca tgctgctggc ggcgattatc
      481 tggaacctgg gaacgtggtt cttcggttta ccggcctcca gttcgcacac cttgattggt
      541 gcgattatcg gcatcggttt aaccaacgcg ctgttaaccg gctcatcggt gatggatgcg
      601 ttaaacctgc gtgaagtgac caaaattttc tcctcgctga ttgtttcccc tatcgtcggc
      661 ctggtcattg cgggaggcct gatattcctg ctgcgacgct actggagcgg gacgaaaaag
      721 cgtgaccgta ttcaccgcat tccggaagat cgcaaaaaga aaaaaggcaa acgtaaaccg
      781 ccattctgga cgcgtattgc gctgattgtt tccgctgcgg gcgtggcgtt ttcgcacggc
      841 gcgaacgacg gacaaaaagg gatcggcctg gtaatgctgg tactggtggg gattgcccct
      901 gctggcttcg tcgtcaatat gaatgcgtcc ggctatgaaa ttacccgtac ccgcgatgcc
      961 gttaccaact tcgaacacta cctgcaacag catcctgaac tgccgcagaa gttgattgcg
     1021 atggaacctc cattgcctgc agcatcgact gatggcacgc aagtaacaga gtttcactgt
     1081 catccggcaa atacctttga tgctattgcg cgcgttaaaa cgatgctgcc aggcaatatg
     1141 gaaagttacg agccgttaac cgtgagtcag cgcagccagc tgcgccgcat tatgctgtgc
     1201 atctctgata cctccgcgaa gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac
     1261 ctgctgaaaa aactgcgcag cgatatgtta agcaccattg agtacgctcc ggtgtggatc
     1321 atcatggcgg tagcactggc gctcggcatt ggcaccatga ttggctggcg tcgtgtagcg
     1381 atgaccatcg gtgagaagat tggtaagcgc ggcatgacgt atgcgcaagg catggcggca
     1441 caaatgacgg cggcagtgtc tatcggcctt gccagttata ttgggatgcc tgtctccaca
     1501 acacacgtcc tctcgtctgc ggtagcaggg acgatggtgg tggacggcgg tgggttacag
     1561 cgtaaaacgg taaccagcat cctgatggcg tgggtgttta ctttaccggc ggcaattttt
     1621 ctttctggtg ggctgtactg gatagcattg cagttgattt aaccttcctg aaaatgcccg
     1681 gtcctggcga tcgggcattt ccatttttga ctagtgataa ccacgcgcgg tcataaaatc
     1741 cgtaatcgct ttttctacat caaccaacac ctgttccagt ggctgattgg catcgatatc
     1801 aaccagttgt gcaccaccaa aggttaactg tggcgttatg gcaatcttcc tcgccagcga
     1861 ttcccgttta tggtcgggtt tacgtgcaca ggcaacttca aggtcaacat tgagtttgat
     1921 gaccagatca ggcttatggc tcgccatcca gtgaaacgct ttacgttcct ggcttgccag
     1981 ccatgagaca aaacgaccac cttcaacgtt aggtgggaac accgtaccat cgtaagcgcc
     2041 aggaatttgg tcctgaggat aacggtcggt tagaacaatt aacccgcgac gacgacaggc
     2101 aagcatatga cgaaagcgca gtaagcgacg ggcgacaaac gctgtaatta ccagcgccgg
     2161 aactggtcca ggcaattttt ttgctgtttt cacctgattt cgttcaattg ttttatgtaa
     2221 ggattttccc atcaacggta attttgtcac tgcacgaccg acatttccgg cctgttttcc
     2281 taaatgaact ctttcggcag caccatattt ttcgacaacg gtaataagat gttcacacac
     2341 cgttgacttg cctgaaccat cgctaccaat aacggcaatt aatggaggtg ttattgactg
     2401 catgaatata tcctttaatt taaatatcca ttaaaaatat ttatttggtt aatatgtttt
     2461 tatgaaagcg taattcaggt caatgtcaca attaaccatt gtcacaataa ggttgaacgg
     2521 atattctgag cacggaccac gtaatattca atacattatt tactgccggg tttttcgtga
     2581 atcaacgtaa tatgtcaata attaattcca cgccagtgca tgttattgcc attgtaggat
     2641 gtgatggttc aggtaaatcg accctcacgg caagcctggt aaatgaactg gcagcaagaa
     2701 tgccaacaga acacatttat ctcgggcaat cgtccgggcg aattggcgaa tggatttcac
     2761 agctccctgt tattggcgca ccttttgggc gttatctgcg aagtaaagcg gcacatgtgc
     2821 acgaaaagcc ctcaacaccg cctggcaata ttactgcact ggttatctat ctgctttcct
     2881 gctggcgggc gtacaagttt cgcaaaatgt tgtgtaaaag ccagcaaggc tttctgctca
     2941 tcaccgaccg ctacccgcag gttgaagtgc cggggtttcg ctttgatggc ccgcaattgg
     3001 caaaaaccac gggcggtaac ggttggataa aaatgttaag gcagcgcgag ttgaagctgt
     3061 accaatggat ggcgtcttat ttgcccgtat tgttgattcg tcttggcatt gatgaacaaa
     3121 ccgcgtttgc gcgtaaacct gaccaccaac tggcagcgtt acaggaaaaa atcgccgtta
     3181 cgccgcaatt gacattcaat ggcgcaaaga tccttgaact ggatggtcgg caaccagccg
     3241 atgaaattct gcaagcgtca ctacgcgcaa tgcacgccgc gctttcctga tcaccaaaat
     3301 ggatagggga tgatttaacg aatggatgca ctacaaacac aaactgttat tagtacaacc
     3361 gcgccgcagc ccaactacat tccggggctg attgcggtgg tcgggtgtga tggcaccggt
     3421 aaatccacac tgaccaccga cctggtgaaa tcgctgcaac aacactggca aaccgagcgg
     3481 cgctatctgg ggctgctctc cggcgaagac ggcgacaaaa tcaaacgatt gccgttggtt
     3541 ggcgtctggc tggaacggcg attggcggcc aaatcctcga aaacccaaag catgaaaacc
     3601 aaatctccag cgctatgggc ggcggtgatt atgtactgct tctcgctgcg aagaatggcg
     3661 aatctacgca aggttcagcg actggcgcaa agtggcgttc tggtggtcag cgatcgcttc
     3721 ccgcaggctg aaatttcggg cttttattat gacggaccgg ggattggcgt cgaacgtgcg
     3781 accgggaaaa tcagcatgtt tctggcgcag cgcgaacggt gtttatacca acaaatggcg
     3841 caataccgtc ctgaattaat cattcgtttg gggattgata ttgagactgc catctcccgc
     3901 aagcctgacc atgactatgc cgagctgcag gacaaaatcg gtgttatgtc gacgattggc
     3961 tataacggca caaaaattct tgagattgat tccagagcgc cctacagcga agtgctggaa
     4021 caggcacaga aggcggtttc tctggttgcc atcgtttctg accgccgaag tttaacttaa
     4081 ggctggcggg atacttttta ctatcaacga taaggtcagg ctgaattggc gggttttaac
     4141 atcaaacatt ggtttgcaga tggcgcgttt cgcaccatta ttcgcaatag cgcctggtta
     4201 ggctccagta atgtcgtgag cgccttgctg gggctgttgg cgctctcgtg tgccggtaaa
     4261 gggatgacgc ccgccatgtt tggcgtactg gtgattgtgc aatcgtacgc caagtcgatc
     4321 agcgatttta ttaagtttca gacatggcag ttggtggttc agtacggaac gccagccttg
     4381 accaacaata atccgcagca atttcgcaat gtcgtctcat tttccttctc gctggatatc
     4441 gtcagcggcg cggtggcgat tgtcggcggc attgccttac tgccattcct ttcccattca
     4501 ttaggtctgg atgaccaaag cttttggctg gcggcgctgt attgcacgct cattccttca
     4561 atggcttcct ccacgccgac cggcattctg cgtgcggtag atcgctttga tttgattgct
     4621 gtacagcagg cgacgaaacc ttttctgcgc gcagcgggga gcgtcgcagc ctggtatttt
     4681 gactttggtt ttgcgggttt tgttattgcc tggtacgtgt cgaatctggt tggcggcacc
     4741 atgtactggt ggtttgccgc gcgcgaatta cgccgcagaa atatccataa cgccttcaaa
     4801 ttgaatctgt ttgagtctgc ccgacacatt aaaggcgcgt ggagttttgt ctggtcaacc
     4861 aacattgccc actccatctg gtcggcgcgt aactcgtgca gcacagtgtt ggtggggatc
     4921 gtgttaggac ccgctgccgc cgggttattt aaaatcgcca tgacattctt cgacgccgcc
     4981 ggaacgccag cgggtttact gggtaaaagt ttttacccag aggtgatgcg tttagatccg
     5041 cgcaccacca gaccgtggtt gttaggcgtg aagtccggtt tactggcggg cggaatcggt
     5101 attctggtgg cgctcgcggt gttgatcgtc ggcaagccgc taatttcgct ggtgtttggc
     5161 gttaagtatc tcgaagcgta tgacctgatt caggtgatgc tgggcgcaat tgtgatctcg
     5221 atgctgggct tcccgcagga gtcattgcta ttgatggcgg gaaaacagcg cgcttttctc
     5281 gtggcgcaaa ccatcgcctc aatcggctac atcgtattgc tgttcatgtt ctgtcatctg
     5341 tttggcgtgc tgggggctgc atttgcttac ttcggtgggc aatgtctgga tgtggtgctc
     5401 tcgttaattc caaccttaaa ggcttttttt cagcgtcatt ccttgcttta taacgcagcc
     5461 ggagagaaat cctgatgatg agaaaatatt tcccgcttga ggcgagcgag cgtctatttg
     5521 ttgcgattga agaagatgat gtcgttgatg cacaggtcag tttgccgccg actattgcac
     5581 taagttgtac caccgaaatc attcatgata attacgcgtt atgcctgcaa ttctggctca
     5641 atggcgttga tcgtcaggaa ttactgcgtt tagttcgcaa acaagccaaa ggcgatgagc
     5701 tgactgctga tgagcgcaaa caatttaaat acatgcgagc gcgctacaag cacctgcgtt
     5761 ttgcgcaacg gctgtattta aagaagcatc aggccggatt tttattcggc aaaacgaccg
     5821 tttttctggg acgttttcag gacggatttc gtaacggtaa gaaaaatatt gtgtcatatt
     5881 acggcaacct gttgcggatt tatttaagct ctccggtctg gtcgttggtg aactattcat
     5941 tacgccatag ccagttagaa agcgtaagcg gttttattgc ctatcggcaa aagcaaatgc
     6001 atgccctgaa agagataatt gccaaaccgc gactaaccgg cagggaattt catgatgtgc
     6061 gaaaaatcat cagccagcag gtttcttatt acgatacctt aagatcgctt gatcctgaaa
     6121 ataaagaggc gttacagatt tcccgtttcc tcgccgccat taatggcctg atgggggata
     6181 aacacgacga catggtggcg gatgatatgg aaaaccgcca gtcgtatgat gcaccggtgg
     6241 cgctggatag cgatattcgc cagcgcttag agctgctgat ttcgcgtttt ccgctataac
     6301 cggagcaatg atgtcatctc gtttgattat tgcgctgatt atcatgctct tagccccggg
     6361 tgttcaggca cacaatttcg tcactggcaa aacggttacc cccgtttata tccaggaagg
     6421 cggcgagctg cttctgaata gtgacgacga aattcactac caaaagtgga acagcacaca
     6481 attggcggga aaagtccgca ttatccagta tatcgccggg cgtaagtcgg cgaagaaaaa
     6541 gaactcactg ttaattaaag cggttgaagc ggcaaacttt ccgcaggatc gttttcagcc
     6601 caccaccatt gtgaacaccg atgatgccat ctttggtact ggctattttg ttgttggcaa
     6661 aatcgaaaaa aataaacgcc gctatccgtg ggcgcaattc gttattgacg gcaatggaca
     6721 gggacgcgtt gcctggcgct tgccggagca gagttcaacc attctggtgc tgaataaagc
     6781 cgggcaaatt cagtgggcaa aggacggcag cctgacgccg gaagaggtag atcatgtcat
     6841 tgcgttggcg cagaaactga tcaatgaata aaaaactcaa aggattgagg gcgttagtga
     6901 gatgaagctg gttgaacact acatcatgcg tggtacgcgc cgtctggtgc tgattatcgt
     6961 cggttttctg atctttattt tcgccagcta ctcctcacag cgttatctga ccgaagcggc
     7021 aaatgggaca ttagctcttg atgttgtgct ggatatcgtt ttctacaaag tgctgattgc
     7081 actggagatg ttgttacctg ttggtctgta tgtgtcagtt ggcgtgacgc tagggcagat
     7141 gtataccgac tcggaaatta ccgctatctc tgcggcgggg ggcagtccgg gacgcttgta
     7201 caaagccgtt ctttatctgg cgataccgct aagtattttt gtcacccttc tgtcgatgta
     7261 tggtcgtccg tgggcttatg cgcagattta tcaactggag cagcagtcgc agtcggagct
     7321 ggatgttcgc cagttgcggg caaagaaatt taacactaac gataacggac gaatgatcct
     7381 ttcgcagacg gttgatcagg ataataatcg cctgactgac gcgctgattt atacttctac
     7441 tgccaatcga acccgcattt tccgcgcccg ttcggttgat gtggttgacc catctcccga
     7501 gaaaccgacc gttatgttgc acaacggcac cgcctatctt ctcgatcatc aggggcgtga
     7561 cgacaacgaa cagatctacc gtaatctgca attacatctg aatccgctgg atcaaagccc
     7621 taacgtcaaa cgcaaagcaa aatcggtcac ggagctggcg cgctccgtct ttccggccga
     7681 tcatgccgaa ctgcaatggc gacaaagccg tggcctgacc gcattgttga tggcgctgct
     7741 ggccatttca ttaagtcggg taaaaccgcg gcaagggcga ttttcaacgt tattgccact
     7801 gacgttgctg tttgttgcca ttttttatgg cggcgacgtc tgccgtacgc tggtggctaa
     7861 cggcgcgatt cctctcattc ctggtttgtg gttagtgccc ggactcatgc taatgggcct
     7921 gctgatgctg gttgcacgcg acttcacttt gctgcagaaa ttttcccgat gaatgttttc
     7981 agtcgctatt taatccgtca tctctttctc ggttttgccg ccgccgcagg gctattgctg
     8041 ccgcttttta ccaccttcaa cctgattaac gaactggatg atgtcagccc gggcggttat
     8101 cgctggactc aggcggtgct ggtggtgcta atgaccttac cgcgcacact ggtcgaactt
     8161 tcgccgttta tcgccttatt gggagggatt gttggcctgg ggcagttatc gaaaaacagt
     8221 gagcttaccg ccattcgcag cacggggttt tctatcttcc gtattgcact ggtggcgctg
     8281 gttgccggga tattgtggac tgtttcgtta ggcgcgatag atgagtgggt ggcgtcgcca
     8341 ttgcagcaac aggcgctgca aatcaaatcg accgccaccg cgttggggga ggacgatgac
     8401 attaccggca atatgctgtg ggccaggcgc ggcaatgaat ttgtgacggt gaaatcgctg
     8461 aatgaacagg gccagcctgt gggcgtggag atttttcatt atcgtgacga tctttcgctc
     8521 gaatcctaca tttatgcacg cagtgccacc attaaagacg acaaaacgtg gatcctgcat
     8581 ggtgtgaatc ataaaaaatg gcttaacggt aaagaaacgc tggaaacatc agataatctt
     8641 gcctggcaat cggccttcac cagtatggat cttgatgagt tatcgatgcc ggggaatact
     8701 ttttctgtcc gtcagcttaa tcattacatc cattatttgc aggaaaccgg gcaacccagc
     8761 agcgaatacc gccttgcact gtgggaaaaa ctggggcaac cgatcctgac cctggcgatg
     8821 attttgctgg ctgtgccgtt tacctttagc gccccgcgct cgccagggat gggtagccgt
     8881 ctcgctgtag gcgtcatcgt tggcttactc acctggatca gctatcaaat catggtcaat
     8941 ttgggattgc tatttgcgtt aagcgcacct gttaccgcgc tcggtttacc ggtagcgttt
     9001 gtgttggtgg cgttgagcct ggtgtattgg tatgacagac aacattaaac ccgccgtggc
     9061 gcaaacacca cggcgggctc acattactgc ttcagagtag caaatgcctg aatgatggca
     9121 tcgatttgct cacgactgtg ggcagcattt acgctacagc gcagcagcgt gatgcctgca
     9181 ggcgcggcag gcggcaggat aagattcaca tacacgccca gcgagataag ctcgcgccag
     9241 atgcgtaaac cttcctcttt cgaaccgatt attactggca ctaccgggct aatatgtgaa
     9301 cttaactcat aacccagttt ttgcagaccg tcatacagat ggttggcgtt atccatcagc
     9361 ttttgccgca gctctggttg tgtggcaatg gttcttaacg atgagcgcac ggtcgcaatg
     9421 caggacggag aaggagaggc ggtaaaaata tacgggcggc tgccgtagcg cagcacttcc
     9481 atcgcttccg aaccgacagc aaaaccgcca atcgaggcca gacttttact gaaagtacca
     9541 acgataatat ccacatcatc ttcaacgccg acagcttcag ccagtccgcg ccccgttgcg
     9601 cctaacacgc caaacgaatg cgcttcatcg acaatcaaat aaccaccgag acgacgtttg
     9661 atatcaacaa tttccgccag cggcgcaacg tcaccgagca tactgtagat gccctcaacg
     9721 atgataatcg cctctttcgc ccgttcaccg aggcgaacca tgcgccgttc cagatcttta
     9781 gcatcgttat ggcggaaacg aataatttcc gcgccaccga gagaacaggc gtcataaata
     9841 ctggcgtggc tgtcggcatc cagtaacacc acggcgttat ggtcggcaag tgcgctgatg
     9901 acgcctaaat tagcggtata accggttgaa aaaacgatag cggtagggcg attgaaaaac
     9961 tcggcaatct ctttctctaa cgccaggtgc ggcgcataac tgccgtttgc catacgagaa
    10021 ccggtggtgc cggtgccttg ggccgctaac gctgcctgac cttcggcgat ggcatcatga
    10081 ttaaaggtta aaccgaggta attattggtc ccggccagaa tcactttttt attgccgatc
    10141 cggccttcgg tggcggaata cacttcatca atacaggtgc caaacggtgt taacccgttg
    10201 tcactaaatt gcagtcgctc gccagcaagg cgcgcatatt tatcgtatag ccccattatt
    10261 tattctccag ccatggaagt agagtttcca ttaattgcgc aggggttttg acatccagca
    10321 gaatattaat aggaatggaa atatcgaatc tgtcttccag catcattaag agatccatca
    10381 ctttgatcga ctccaggcca agatcgttga cgagatcgct gtcgggctta atttccacac
    10441 cgttttcgac caaatcctgt aaacaggaaa ggatgtaatc cattactatt tcacgattta
    10501 ccatagaaac gttaacacac ctttaaacaa gacaatcaaa atagcccgtt gcgtaatgcg
    10561 tgttccaggc taatcccggg aaaccaatta atatcttcag aaatacggtt attacttgcc
    10621 gaccagtcgg cgtgggttaa ttcacgaatt ttcgagcggg tcagcatagg ttctttaccc
    10681 gccaggcgac tcaacgcagt actgatatcc gcaaggcagg tgagtaccgg cagaggaata
    10741 ccaaccattc gcacggaacc gcaacggacg ttggcggcaa gttgctgtat gcgttgccag
    10801 tcatagccgc cagcgacgcc atcgcataat tcataggttt gcgtctgtac agtttcggcg
    10861 cttaaccact gacccactgc ttgcgcaaaa tcggtgacgt gcaggaaaga gagctgtgta
    10921 tctggtgcac caagtcgtgg cagcaggccg cgcagcatcc agtcaaacag cggttttaac
    10981 tctttatcgc cgggaccata cacggctgtc gggcgaaaaa cgcccagcgt aatttcgtca
    11041 gccattgcag tcagccgttg ttcggcgatg tgtttggaat ttgcgtacca ggagagctcg
    11101 ggatggcgcg ccgccagcga agagataaac agaaaacgtt ggcaaaagca gctctctttt
    11161 gctgcttgca tcaggcgcag gctgccgtca acgttacagc gggtgaaaat ctcttctttg
    11221 tgcccgcgca cttgcccggc gcaatggacc actgcgctgg ctccggcaac cagctcgcta
    11281 agtgaatgag tatcttccag cgaaccgcgc acccaggtga gattatcatt gacgtgagcg
    11341 cgggcagtac gcgtcaatgc gcgaacgtga aagccgcggg cgagcaggtt atcgataata
    11401 tatttaccga taaacccggt agcgcccgtc accgcgacag tttgattcat gccagggatt
    11461 cctgcacatt aagactggca gcataagcct tctgataacg ttttttcgct tccgcacggg
    11521 caggcttgcc ggaggacgtt cggggaatac tgtggggcgg caacagatcg atagccgcgg
    11581 tcacgccaaa ttcgctttgg atccgtgccg ccagcgcgtg gataagctgc ccgcgacgtt
    11641 cttcgtcgct gatccgacac tggatctgca aaatgatttt ttcctgggcg gtaacaaaag
    11701 caatcgcatc gccagaatga atttccggtt cttgttccgc aatatattca atatcctgcg
    11761 gccagatatt acggccacga ataataatca gatctttaat gcgtccggtg acatacagat
    11821 aaccgtccag cagataaccg aggtcgccgg tgtctaacca gcccgtcgcg gcaatctcgt
    11881 cttgcgaaac ctggtcgcca aagtaaccgc tcatcagact gggaccggag atgcaaatat
    11941 ggcctaccac acgttccgcg accggcatac ctgcttcatt gcggatttca ataccatgtt
    12001 ccggcaacgc tttgccgcag ttgacgaaag tcgatacggc gcgtgtctct gcacccggcg
    12061 cgacggcttt gccctgatat tcgaggatgt cgcgatccac ttcgttaacc acaaccccgg
    12121 aggcttcatc agagaagctg acagccagca cattttctgc cagtccgtag cacggcatga
    12181 acgttttatc gtcaaagtta acctgacgga aacattcagc gaattgatgg agttgttctg
    12241 cggagatcgg ctcagcacca ataccagcga cgcgccagca ggaaagatcc agttcagcga
    12301 gatctttttc attcacgcgg cgctggcaca attcatagcc aaacggcggc gcaacggaaa
    12361 cggtgccgcg atttttactg atcaatttaa gccattgcag aggacgcatg gcaaaatcct
    12421 gagtgcgcaa ataatctact gaaagttgcg tggcgacggg ggtcaggaga aagccgacca
    12481 gtcccatatc atggtagaaa ggcagccagg agacgcagcg gtcgccaggg cgtaatttaa
    12541 tgccatcgtg gcttatagca cgtagattag ccatcacttc gcgatgggtg ataatgacgc
    12601 cacggggaaa acgggtgctg ccggaggtgt actggaggta ggcgatatcg ttcggaactg
    12661 gacgctggag cgcaacatcg gcttccggta atgccttaaa ccaggcgtgg cttaaaacat
    12721 gtaattcggg gttgttatgc gtcgcggcat tgaccagtgg caaccactca tcaccagtga
    12781 taatggctgc gggctggcaa cttgccagta aaccctggag tttggcgctc caggaatccc
    12841 gctgaccgac gcccattgga atcgccaacg ggacggcgac taagccagca tactgacagg
    12901 caaaaaaagc ctctacaaac ccgctactcg tttcggcaat cagcgccacg cgttcgcctt
    12961 ttttcaggtt cagcgataac aaccgcttcg caccagcttc ggcacgcgtt tttaacgttt
    13021 gatattccag ttgatcttca agttggcaac gtctgtcata aaaattcatt ccggcgctac
    13081 tcagagcggc gtagtccaaa gcatcaacca gcgttggaaa atcggcatag cgcataggta
    13141 gggaatgcgt aaagatttta ttagacatat acaccacgaa aaaattggat atcaaaaata
    13201 aaatga
//
LOCUS       DAJOSY010000114        13183 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJOSY010000114 DAJOSY010000000
VERSION     DAJOSY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.114"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(154..366)
                     /gene="cspH"
                     /locus_tag="OHN27_003595"
     CDS             complement(154..366)
                     /gene="cspH"
                     /locus_tag="OHN27_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCQ2287971.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            652..864
                     /gene="cspG"
                     /locus_tag="OHN27_003596"
     CDS             652..864
                     /gene="cspG"
                     /locus_tag="OHN27_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCQ2287972.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            875..1063
                     /locus_tag="OHN27_003597"
     CDS             875..1063
                     /locus_tag="OHN27_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCQ2287973.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1038..1268
                     /gene="ymcE"
                     /locus_tag="OHN27_003598"
     CDS             1038..1268
                     /gene="ymcE"
                     /locus_tag="OHN27_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCQ2287974.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            1258..1431
                     /locus_tag="OHN27_003599"
     CDS             1258..1431
                     /locus_tag="OHN27_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCQ2287975.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1479..2552)
                     /locus_tag="OHN27_003600"
     CDS             complement(1479..2552)
                     /locus_tag="OHN27_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCQ2287976.1"
                     /translation="MAEKKRTSWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(2624..5368)
                     /gene="torS"
                     /locus_tag="OHN27_003601"
     CDS             complement(2624..5368)
                     /gene="torS"
                     /locus_tag="OHN27_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCQ2287977.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSTVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAHAIETLQNSEPFAAALVDFDLPDID
                     GITLARQLLQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5451..6479
                     /gene="torT"
                     /locus_tag="OHN27_003602"
     CDS             5451..6479
                     /gene="torT"
                     /locus_tag="OHN27_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCQ2287978.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6452..7144)
                     /gene="torR"
                     /locus_tag="OHN27_003603"
     CDS             complement(6452..7144)
                     /gene="torR"
                     /locus_tag="OHN27_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCQ2287979.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QSVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7274..8446
                     /gene="torC"
                     /locus_tag="OHN27_003604"
     CDS             7274..8446
                     /gene="torC"
                     /locus_tag="OHN27_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCQ2287980.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TQFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8446..10992
                     /gene="torA"
                     /locus_tag="OHN27_003605"
     CDS             8446..10992
                     /gene="torA"
                     /locus_tag="OHN27_003605"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCQ2287981.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVGWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGRGHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDHYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10989..11588
                     /gene="torD"
                     /locus_tag="OHN27_003606"
     CDS             10989..11588
                     /gene="torD"
                     /locus_tag="OHN27_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCQ2287982.1"
                     /translation="MTTLTAQQIACIYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(11740..12045)
                     /gene="cbpM"
                     /locus_tag="OHN27_003607"
     CDS             complement(11740..12045)
                     /gene="cbpM"
                     /locus_tag="OHN27_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCQ2287983.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12045..12965)
                     /gene="cbpA"
                     /locus_tag="OHN27_003608"
     CDS             complement(12045..12965)
                     /gene="cbpA"
                     /locus_tag="OHN27_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCQ2287984.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3205 a         3360 c         3451 g         3167 t
ORIGIN      
        1 ggctgttaaa tggcatttat aactattagg tgcagagata ttcgcttaaa tggattagtt
       61 tctttgtgga aaagtaactg atgttataat gtatgatgtt gactattgca ctattccaat
      121 aaaaccagtc agctttaaac aagcagcgtc atattaagag agataaacat ttgccgctgt
      181 tggtcctcgc aggccattta cgcggcaaaa ttccacacgt aatcctggta taagcacttc
      241 tgcgtcgcgg ggagtgaatg cggaaatatg gacctgaact tctttacgac cgtcggaggg
      301 gataatgaat cctttgccgc ttttgcgatc aaaggttttg acaattcctg tcattttacg
      361 ggacaaacaa attccttact gaaaatactg cgctgcacta tacggggtta ataaaataaa
      421 gccagcgata tttaagaccg ccggacggct aaaataaaat ttgcttaatc tcaattatca
      481 tgcgttaata gctgcgtcgg tttgaaagac agacagcata caaagtagtt tactaaagca
      541 gttctcatta tcaggcatta tccccttctt ttgagtctct ctcctgaaca ctaagtagtt
      601 tctgtattaa agccctgttt gccgaaaggc ccaaaatgaa ggaagtaaaa tatgtctaat
      661 aaaatgactg gtttagtaaa atggtttaac gcagataaag gttttggctt tatcactcct
      721 gatgatggca gcaaagacgt tttcgtccat ttcactgcca tccagagcaa tgaattccgc
      781 acgctgaacg aaaatcagaa agttgaattt tctattgagc aggggcaacg tggccccgcg
      841 gcagcgaacg ttgttacgct ctaaggttgc cgttattact caacatctcc atttccgctg
      901 tccatgttgt catggttcac agtaccgtac atcggcattc gatgtgacgg agcgaaaccc
      961 tttgggcgct aagtgtattt tttgtaaatc gacgatgatc acctttgata acgtcgcgct
     1021 gcaaatacgc acggaccatg cgccgctgga tttcacaaaa taatatcagg ctccctcgtg
     1081 gagccttttt tatatctgcc ttatttttct tcaacgctgt atgtatagta agcgataacc
     1141 tgttgattat tgaatctttc ggggagatgg cttataacat ttcttacctg accagggtac
     1201 cgggaaccaa caccttactg gcgtgttgct gtctttcaag accagaagag gttaacagtg
     1261 aatattgaag agttaaaaaa acaagccgaa acggaaatcg ccgactttat cgcgcaaaaa
     1321 atcgccgagc tgaacaagaa tacagggaaa gaagtctctg aaattcgctt caccgcacga
     1381 gaaaaaatga ccgggcttga aagttatgat gtcaaaatca aaataatgtg attttgtgaa
     1441 catcacccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
     1501 tgtggaatta agcgtcggta caattcatcc ggtaccgggc tttgccatac tcccgcatac
     1561 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
     1621 gacaacgcaa aggctttttt atttgccgca ggtttttgca gtgaaaaggt caatgttgag
     1681 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1741 attaatttat cgaccgggat ccgtgatggg caattttttg cgcatttgcc acagtcgata
     1801 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1861 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1921 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1981 acgtccatca ccacgctgta gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     2041 aataacgcga tatagagaaa aaaactcaac agcaaatact tcacgccgcg cagaggaata
     2101 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     2161 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     2221 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     2281 cctgttacaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     2341 cctcccggtc tggcgacaaa tgtcgtgcta cttgccgttt cgtaatagcg cacccaatac
     2401 cagaacgtga tggcaatata aatattcatt gccaacagta ataattgcgt cgctttacgc
     2461 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     2521 cgctgccagc tggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     2581 caaatgccac tattctagtt gttcttaact ggctgatatt gattcaaatc gcgttcaggt
     2641 ctttcttatg caaccatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2701 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2761 ccaaactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctatcct
     2821 gactggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt gtaaataata
     2881 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2941 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     3001 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa taatgaactg gtacgctggc
     3061 gcaacgtttc gtcgatgaca tgagcgctaa agccaatcaa aaccagcgac ggatattgct
     3121 gtagcagttg tcgggcaagc gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     3181 cggcagcaaa cggttcacta ttttgcaacg tctctatcgc gtgcgcggca tttcctaccg
     3241 caacgacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     3301 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg actgtttttg
     3361 gcacgggtgc cgtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     3421 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     3481 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     3541 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     3601 catcagtgcg gctacgtaaa actatctgcc cttcgtcagt aaaacgcagg gcgttgctca
     3661 acaggttggt tataacctga cgaatacgtc gcggatcgcc cattaacgcg gtcggtacat
     3721 cgtcggcaat tgctgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3781 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgacc gaaacattct
     3841 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3901 cgccagagtc ggtaatcgcc cgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3961 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     4021 ccgccagaaa ggccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     4081 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     4141 cacggtggcg attcagtgca tgaacagtgc tgcgaaacgc atccatcagc cgcccgatgg
     4201 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     4261 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gactgagcga taaaccacgc
     4321 gccaaaggat cagaatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     4381 gcccgcgtgc actggctttt tccagatgcg ccagtccgtg ctgattacgc agctcaatgg
     4441 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgatg ttattttgtg
     4501 cgagagtttg taggtgattg ctgatttcac tgtcctgctg atacagcgcc agcaaatcgc
     4561 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaacaccc ggatcttcaa
     4621 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4681 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4741 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4801 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4861 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgcgccagg cgtgcgatct
     4921 catcggcggc agcgactatc tgctgactga gttgctgttg ttgctggcgc agttgcaaac
     4981 gctgccccac cagttcccct tgctgacgta acgaacggga gatctcctgc tcctgttgtt
     5041 caatagcggt ggtatcaaaa ccttgttccc gtaacgcttg cagcaacgca ttaatcttca
     5101 ggctttgtgc ggtgagcatt cgcccctgcg cctgccacat cttttcgtta tcggcactgg
     5161 tcaggttctg cgcggcgaaa agttcccagg cgctggcttc gctcaactgg cgcgccatat
     5221 tcatggtagg aatcaatgcc tgagtgttgt ctttttccac ctggctgata aagcgcaggt
     5281 tgtaccatcc caccagggta ctggtcaggg ttaacagcgc catcagggca aagcccatcc
     5341 agagtcttcg ggtcagggtt aaattcacgg tcggtgcact ttaggtgaaa aaggttgagt
     5401 cgcaaagcgg aatgcatcta gcataaagcc ttattattga tgaggctatc atgcgcgtac
     5461 tgctattttt acttctttcc cttttcatgt tgccggcatt ttcggctgat aacctgttgc
     5521 gctggcatga tgcgcagcat ttcacggtgc aagcctctac gccgcttaaa gccaaacgcg
     5581 catggaaact gtgcgcgctt tatcccagcc tgaaagattc atattggtta tcgttgaact
     5641 atggtatgca ggaggctgct cgccgctacg gtgtggattt aaaagtgctg gaggcaggcg
     5701 gctacagcca gttggctacc cagcaagcac aaatcgacca gtgtaaacag tggggcgcag
     5761 aggccatttt gctcggtagt agcacgacct catttcccga cctgcaaaag caggtagcaa
     5821 gtctgccggt gatcgaactg gtaaatgcta ttgatgctcc ccaggtgaaa agccgcgttg
     5881 gtgtgccctg gtttcagatg ggctatcaac cggggcgata tctggtgcaa tgggcgcacg
     5941 gtaaaccact gaatgtgctg ttgatgcccg gacccgataa cgccgggggc agtaaggaga
     6001 tggtcgaggg ttttcgcgca gccattgccg gaagcccggt gcgtattgtt gatattgcgc
     6061 ttggtgataa cgatattgaa atccagcgta acctgttgca ggagatgctg gaacgccatc
     6121 cagaaatcga cgtcgttgcc ggaacggcca ttgcggcaga ggcggcaatg ggggaagggc
     6181 gtaacctgaa aacgccgctt accgtggtgt cgttttatct ttcacatcag gtgtatcgcg
     6241 ggctgaagcg gggaagagtg attatggctg ccagcgatca aatggtctgg cagggggaac
     6301 tggcagttga gcaggccatc aggcaattac aggggcaatc ggtgtctgat aatgtcagcc
     6361 caccgatttt agttctgacg ccgaaaaatg ccgaccgaga acatatccgc cgctcgctgt
     6421 caccgggggg atttcgtccg gtctattttt atcagcacac atcagcggct aagaaataac
     6481 cttcaccatg ttgcgtcacc agtaaatccg cgctgagttt atgacgtaaa cgacgaatta
     6541 acacatcgac ggtgcgcagg tcagggtttt ccacccgacg cgcagaaagc atacgtagca
     6601 gacgttcacg gctgagaatt tcgcccggat tcgtcacaaa tgccaccaac atttcatact
     6661 ctgcgcgggt cagtttaatc ggctcaccat cccgctccag cgtatgacgc gacacattca
     6721 ggcaataacc cgcaaagcga tagcagttgt cctgagtgtg cggttgagct tgtcgcgcga
     6781 ggtcgattcg ccagagcaga tttttcaccc gtactaccag ttcgcgcagt tccagcggtt
     6841 tggtgacgta atcgtctgcg cccatttcca gcccaacaat acggtcaatc cgatcgctgc
     6901 gtccggtaac cagaataatc cccaccgttg agcgttctcg cagggcgcgg gttaacatca
     6961 ggccattttc atcgggtaag ttgatatcca gcagaattaa atctaccgac tgattctgca
     7021 taatttcccg tagcccggca ccgctcgccg taacggaaac ggtatacccc tcctgagtga
     7081 agtaggattg taatcgcgcc tgggtaaccg gctcatcttc aacaataaca atgtgatgtg
     7141 gcatcagagg gttttactca ttctgttcat atctgttcat attctgccgt aagccgttca
     7201 tcctgaccag tgccgctgtt catatttgct cattaagatc gcttcactaa accataattc
     7261 tacaggggtt attatgcgga aactctggaa cgcgctacgc cgacccagtg ctcgttggtc
     7321 ggtactggcg ctggtcgcaa ttgggattgt gattggcatt gcgctgattg tattgccaca
     7381 cgttgggatc aaagtcacca gcacaaccca attttgtgtc agttgccaca gtatgcaacc
     7441 ggtgtatgaa gaatataaac agtcggtgca tttccagaac gcctccggtg tgcgagctga
     7501 atgccatgac tgtcatatcc cgccggatat tccaggcatg gtgaagcgca aactggaagc
     7561 gagcaacgac atttaccaga cctttattgc tcactccatt gatacacctg aaaaattcga
     7621 agccaaacgc gcggaacttg ccgagcgtga atgggcgcga atgaaagaaa acaactcggc
     7681 aacctgtcgc tcctgccata actacgatgc gatggatcat gccaagcagc atcctgaagc
     7741 ggcacgtcag atgaaggtgg cagcgaaaga taatcaatcc tgcatcgact gtcataaagg
     7801 tattgcccac cagttaccgg atatgagtag cggcttccgt aagcagttcg atgagctgcg
     7861 cgccagtgct aatgacagtg gtgacacgct gtactctatt gatattaagc cgatttatgc
     7921 ggcgaaaggc gataaagaag cctctggttc tctgctgcct gcttcggaag tgaaagtcct
     7981 taaacgtgac ggcgactggc tgcaaattga aattaccggc tggacggaaa gcgccggacg
     8041 tcagcgtgta ctcacccaat tcccaggtaa acgcatcttt gttgcctcga ttcgtggtga
     8101 tgtgcagcag caggtaaaaa cgctggagaa aaccaccgtt gccgacacca ataccgagtg
     8161 gagcaagttg caggccaccg cgtggatgaa gaaaggtgac atggtgaacg atatcaaacc
     8221 gatctgggct tatgcggatt cgttgtacaa cggcacctgt aaccagtgcc acggcgcacc
     8281 ggaaatctct cactttgacg ctaacggctg gattggcacg ctcaacggca tgatcggctt
     8341 taccagcctc gataaacgtg aagaacgcac cttgttgaaa tatctgcaaa tgaatgcgtc
     8401 tgacaccgca ggtaaggctc acggcgataa gaaggaagaa aaataatgaa caataacgat
     8461 ctctttcagg catcacgtcg gcgttttctg gcacaactcg gcggcttaac cgtcgccggg
     8521 atgctggggc cgtcattgtt aacgccgcgc cgtgcgactg cggcgcaagc ggcgactgag
     8581 gctgtcatct cgaaagaggg cattcttacc gggtcgcact ggggggctat ccgcgcgacg
     8641 gtgaaggatg gtcgctttgt ggcggcaaaa ccgttcgaac tggataaata tccgtcgaaa
     8701 atgattgccg gattgccgga tcacgtacac aacgcggcgc gtattcgtta tccgatggta
     8761 cgcgtgggct ggctgcgtaa gcgccatctc agcgatacct cccagcgcgg tgataaccgt
     8821 tttgtgcgcg tgagttggga tgaagccctc gacatgttct atgaagaact ggaacgcgtg
     8881 cagaaaactc acgggccgag tgccttgctg accgccagtg gttggcaatc gacggggatg
     8941 ttccataacg cttcggggat gctggcgaaa gctattgcct tgcatggtaa tagcgttggt
     9001 acgggcggag attactctac cggtgctgcg caggtgatcc tgccgcgcgt agtcggttcg
     9061 atggaagtgt atgaacagca aacctcctgg ccgctggtat tgcagaacag caaaaccatt
     9121 gtgctgtggg gctccgattt gctgaaaaac cagcaagcga actggtggtg cccggatcac
     9181 gatgtttatg aatattacga gcagctaaaa gcgaaagtcg ccgccggtga aattgaggtc
     9241 atcagcatcg atccggttgt cacatccacc catgagtatc tggggcgcgg gcatgtgaag
     9301 cacatagcgg ttaacccgca aactgacgtg ccgctgcaac tggcgctggc gtatacgctg
     9361 tacagtgaaa acctgtacga caaaaacttc ctcgctaact actgtgtagg ttttgagcag
     9421 ttcctgccgt acctgctggg tgagaaagac ggtcagccga aagatgccgc atgggctgaa
     9481 aaactgaccg gcattgatgc cgaaaccatt cgtgggctgg cgcgccagat ggcggcgaac
     9541 agaacgcaga ttattgctgg ctggtgcgta cagcgtatgc agcacggtga acagtgggcg
     9601 tggatgattg tggttctggc ggcgatgctg gggcaaattg gcctgccagg tggtggcttt
     9661 ggttttggct ggcactataa cggcgcaggc acgccggggc gtaaaggcgt tattctgagt
     9721 ggtttctccg gctctacgtc gattccgcct gttcacgaca acagtgatta caaaggctac
     9781 agcagcacca ttccgattgc ccgttttatc gatgcgatcc tcgaaccggg gaaagtgatc
     9841 aactggaacg gtaaatcggt aaaactgccg ccgctgaaaa tgtgtatttt tgccggaact
     9901 aacccgttcc atcgccatca gcagatcaac cgcatcattg aaggctggcg caagctggaa
     9961 acggttatcg ccatagacaa ccagtggacc tcaacctgcc gctttgccga tatcgtgctg
    10021 cctgcgacca cgcagtttga gcgtaacgat ctcgaccagt acggcaacca ctccaaccgt
    10081 ggcattatcg ccatgaaaca ggtggtgccg ccgcagttcg aggcgcgcaa cgactttgat
    10141 attttccgcg agctgtgccg tcgctttaat cgcgaagaag cctttaccga agggctggac
    10201 gaaatgggct ggctgaaacg catctggcag gaaggtgtac aacaaggcaa aggacgcggc
    10261 gttcatctgc cagcgtttga tgacttctgg aataacaaag agtacgtcga gtttgaccat
    10321 ccgcagatgt ttgttcgcca ccaggcattc cgcgaagatc cggatctcga accgctgggc
    10381 acgccgagtg gcctgattga gatctactcg aaaaccatcg ccgatatgaa ctacgacgat
    10441 tgtcaggggc atccgatgtg gtttgagaaa atcgaacgct cccacggtgg gcccggctcg
    10501 cagaagtatc cgttgcatct gcaatctgtg catccggatt tccgacttca ctcgcagtta
    10561 tgtgagtcgg aaactctgcg tcagcaatat acggtagcgg gtaaagagcc agtattcatt
    10621 aacccgcagg atgccagcgc gcgcggtatt cgtaacggtg atgtggtacg cgtctttaac
    10681 gctcgcggtc aggtgttggc aggggcagtg gtttctgacc actatgcacc cggcgtggca
    10741 cgaattcacg aaggggcatg gtacgatcca gataaaggcg gcgagcctgg tgcgctgtgc
    10801 aaatacggta accccaacgt gttgaccatc gacatcggta cttcgcagct ggcgcaggcg
    10861 accagtgcgc acactacgct ggtggaaatt gagaagtaca acggaacagt ggagcaggtt
    10921 acggcgttta acggccccgt ggagatggtg gcgcagtgcg aatatgttcc cgcgtcgcag
    10981 gtgaaatcat gaccacgctg acagcacaac agattgcctg tatttacgcc tggctggcgc
    11041 agttgttctc ccgtgaactg gacgatgaac aactgacgca aatcgccagt gcgcagatgg
    11101 ctgaatggtt ttcgttgctg aaaagcgaac cgccgctcac tgcggcggtg gatgagctgg
    11161 aaaaccgtgt tgccacgctg acagtacgtg acgatgcccg tctggaactg gccgctgact
    11221 tttgcggcct gtttctgatg actgacaaac aagcggcgct gccgtatgca tcggcctaca
    11281 aacaggacga gcaagagatt aaacgcttgt tagttgaggc agggatggaa accagcggca
    11341 atttcaacga accggcagat catctggcga tctatctcga gttgttgagt catctgcatt
    11401 tttcgctggg agaggggacg gttcctgcgc gaagaatcga cagtttacgg caaaaaacac
    11461 tcacggcgct gcggcaatgg ttaccagagt ttgttgcgcg ttgccatcag tatgacagct
    11521 ttggttttta cgcggcacta agccagttat tgctggtgtt agttgagggc gaccaccaaa
    11581 acagataacg tcgtttgttg gcctgataag acgcggcagg cgtcgcatca ggcattatgg
    11641 tagagccgcc ggatgcggcg tgaacgcctt atccggccca caggaaaagt actctttgca
    11701 ggccggctaa gatgcgtcat cacatccggc aaacacacat cacggatgag ctacaaaccg
    11761 ggaaagccgc tggcgcagca ggcggttttc ctgcttcagg tgcgcaatat catccattaa
    11821 cgtcagcgcc accgcgatcc ccggccagtc cagagccagt tcatgacgca ggcgcaccgc
    11881 gcgttgcacc acgatggcgg catggtcgtc aaatacccag gttgtttcct ggatctcacg
    11941 cggttcaacc acccccaaac cgacaatttc attcaattcc tcttcagaga tgccggtatg
    12001 caggcaaaat tcggtaatag taaaagtcac cgtaacatta gccattatgc tttcccccaa
    12061 tctttacgtg gatcaaaaga cgactgggcg tctgccagtt gctgccacag cgcggcagtg
    12121 ttttcatccg gtttcggcgg catcacgatt ttcagtaccg catacagatc gccggtctgt
    12181 tttttgctca ccagaccttt gcctttaacg cgcaatcgtt gcccagcctg gctgccaggc
    12241 gggatagtca gcaaaatgct ttctttcagt gttggaacgg tgactttagc acccagcgcc
    12301 gcttcccacg ggctaaccgg caccacaatt tccagatcct ggccgacaat atcaaacagc
    12361 ggatgtggcg caatatgaat caccagccac aaatcgccat ttggaccgcc gttttcgccc
    12421 ggcgtcccct ggcctttcag gcggatgcgt tgaccattgc cgacacctgc cgggatcttc
    12481 acattcagcg ttttcgggat ttcctgttcg atcatgccaa aggcgttata aaccggcagg
    12541 ttatagctga tggtacgctt atgctcagta agcgtttctt cgaggaatac cgccacttcg
    12601 atttcaatat cgtggccgcg tgcggcgggg cgttgacggc tctggcgggc atgctgaccg
    12661 aaaattgacg agaagatatc gtcaaaatct tcggcattaa aactctgacc gtcgccatga
    12721 tggaactgac ggttaaattg cggatcgttg cgatgttgcc acatctgatc atactcagcg
    12781 cgacgttgtt catcacttaa cacttcccag gcttcagcga cctctttgaa gcgggcttcg
    12841 gcatccggtt ctttgctgac atcaggatgg tatttacggg ccagtcgacg ataggcggtc
    12901 ttgattgtct tgagatcgtc cgtcggtttc acgcccatga tggcgtaata atcctttaat
    12961 tccatagcgt tatctcgcgt aaatcaacac aaattgaagg aacccctgta aggtaactcc
    13021 tataagtgta gggtaatcct caaaatttca tatgccaaca cagaatatgt tattgaaatc
    13081 atcgcggaga ggaggtcgcc tcaaggtggg gtgctgaaca tattttaaac aggtgaaaaa
    13141 gggtgagcga tttttgatag tggaaccagg cactttaagt tta
//
LOCUS       DAJOSY010000115        13041 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJOSY010000115 DAJOSY010000000
VERSION     DAJOSY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.115"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            123..836
                     /gene="aphA"
                     /locus_tag="OHN27_003609"
     CDS             123..836
                     /gene="aphA"
                     /locus_tag="OHN27_003609"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCQ2287985.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWQQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            947..1364
                     /locus_tag="OHN27_003610"
                     /pseudo
     CDS             947..1364
                     /locus_tag="OHN27_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
     gene            1368..1724
                     /locus_tag="OHN27_003611"
     CDS             1368..1724
                     /locus_tag="OHN27_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCQ2287986.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(1759..4581)
                     /gene="uvrA"
                     /locus_tag="OHN27_003612"
     CDS             complement(1759..4581)
                     /gene="uvrA"
                     /locus_tag="OHN27_003612"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCQ2287987.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            4835..5371
                     /gene="ssb1"
                     /locus_tag="OHN27_003613"
     CDS             4835..5371
                     /gene="ssb1"
                     /locus_tag="OHN27_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCQ2287988.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5470..5751)
                     /locus_tag="OHN27_003614"
     CDS             complement(5470..5751)
                     /locus_tag="OHN27_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCQ2287989.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6181..7767
                     /gene="pdeC"
                     /locus_tag="OHN27_003615"
     CDS             6181..7767
                     /gene="pdeC"
                     /locus_tag="OHN27_003615"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCQ2287990.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(7770..8093)
                     /gene="soxS"
                     /locus_tag="OHN27_003616"
     CDS             complement(7770..8093)
                     /gene="soxS"
                     /locus_tag="OHN27_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCQ2287991.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8179..8643
                     /gene="soxR"
                     /locus_tag="OHN27_003617"
     CDS             8179..8643
                     /gene="soxR"
                     /locus_tag="OHN27_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCQ2287992.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9189..10538
                     /gene="ghxP"
                     /locus_tag="OHN27_003618"
                     /gene_synonym="yjcD"
     CDS             9189..10538
                     /gene="ghxP"
                     /locus_tag="OHN27_003618"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCQ2287993.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10690..12339
                     /locus_tag="OHN27_003619"
     CDS             10690..12339
                     /locus_tag="OHN27_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCQ2287994.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12493..>13041)
                     /locus_tag="OHN27_003620"
     CDS             complement(12493..>13041)
                     /locus_tag="OHN27_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCQ2287995.1"
                     /translation="FYMAYMAEVNLYKVIAPYVNLFKADLSFSKLDLINFENADLSRV
                     NLNKSILQNINLIDSKLFFTRLTNTFLEMVICTDSNMANVNFNNANLSNCHFNCSVLT
                     KAWMFNTRLYRVNFDEASVQGMGISILRGEENIPINSDTLVTLQKFFEEDCATHTGMS
                     QTEDNLHAVAMNITADIMQHAD"
BASE COUNT         3040 a         3199 c         3430 g         3372 t
ORIGIN      
        1 ttttcatcac aaaaaccgca gataatcctt cctttccccg gcagctggcg ttatggtcag
       61 atggtttttg caacaaatct cacaataaaa agtttcaaca tactgactat ttagggaaaa
      121 atatgcgcaa gatcacacag gcaatcagtg ccgtttgctt attgttcgct ctaaacagtt
      181 ccgctgttgc cctggcctca tctccttcac cgcttaaccc tgggactaac gttgccaggc
      241 ttgctgaaca ggcacccatt cattgggttt cggtcgcaca aattgaaaat agcctcgcag
      301 ggcgtccgcc aatggcggtg gggtttgata tcgatgacac ggtacttttt tccagtccgg
      361 gcttctggcg cggcaaaaaa accttctcgc cagaaagcga agattatctg aaaaatcctg
      421 tgttctggga aaaaatgaac aatggctggg atgaattcag cattccaaaa gaggtcgctc
      481 gccagctgat tgatatgcat gtacgccgcg gtgacgcgat cttctttgtg actggtcgta
      541 gcccgacgaa aacagaaacg gtttcaaaaa cgctggcgga taattttcat attcctgcca
      601 ccaacatgaa tccggtgatc tttgcgggcg ataaaccagg gcaaaataca aaatcgcaat
      661 ggcagcagga taaaaatatc cgaatttttt atggcgattc tgataatgat attaccgccg
      721 cacgcgatgt cggcgctcgt ggtatccgca ttctgcgcgc ctccaactct acctacaaac
      781 ccttgccaca agcgggtgcg tttggtgaag aggtgatcgt caattcagaa tactgacaga
      841 gcgggagagc gtgatgctct cccgaatgct gtttttttaa tcacaccttt atcctttcgc
      901 tgtcttgctg caaactgatt aagagagttt tatcaaggag cagcacatgt ggtatcaaaa
      961 gacgctcacg cttagcgcca aatctcgtgg gtttcatctg gtaacggatg aaattctgaa
     1021 tcagctggct gatatgccgc gcgttaacat cggcttactg catctgttgc tgcaacatac
     1081 ctccgcctct ctgacactta atgagaactg cgatcccacc gtacgccacg acatggagcg
     1141 tttttttcct ccgcaccgtt cccgacaacg gaaattatga gcatgactat gagggagcag
     1201 acgatatgcc ttctcatatc aaatcctcaa tgctgggaac atcgcttgta ttgccggtgc
     1261 ataaagggcg tattcagacc ggcacctggc aaggcatttg gctgggggaa catcgcatcc
     1321 acggcggatc gcgtcgcatc atcgcgacac tacaagggga gtaaaaaatg accatttcgg
     1381 agttgctaca atattgcatg gcaaaaccag gcgcagaaca gagcgtgcat aacgactgga
     1441 aagcgacgca gatcaaagtg gaagatgtac tgtttgcgat ggtgaaagaa gtagaaaatc
     1501 gcccagctgt ttcgctgaaa accagcccgg agctggcgga gctgctacgt cagcagcaca
     1561 gcgatgtgcg tccaagccgc catctgaata aagcgcactg gagcaccgtg tatctcgacg
     1621 gttcgctgcc agattcgcaa atctattatc tggtggatgc gtcttatcag caggcggtga
     1681 atttactgcc ggaagaaaaa cgtaaattgc tggtgcaact ctgaaaggaa aaggccgctc
     1741 agaaagcggc cttaacgatt acagcatcgg cttaaggaag cgtgccgtgt gtgatgcttc
     1801 gcactccgcg acggtttctg gcgtaccgga gacgaggatc tcgccgccac cactgccgcc
     1861 ttctggtccc aggtcgacaa tccagtcagc ggttttgatc acgtcgagat tgtgctcaat
     1921 caccacaatg gtgttgccct gatcgcgcag tttatgcagt acgtcgagca gttgctgaat
     1981 atcggcgaag tgcagaccgg tggtcggctc gtcgagaata tacagcgtct gcccggtgcc
     2041 gcgttttgac agttcacgcg ccagcttcac gcgctgggct tcaccgcctg aaagggtggt
     2101 tgcggactgc cccagtcgaa tgtacgtcag gccaacgtcc atcaacgttt gcagcttacg
     2161 cgccagtgca ggtacggcat caaagaactc acgcgcctct tcgatggtca tatccagcac
     2221 ttcgtggatg gttttgcctt tgtacttaat ctccagcgtt tcacggttat agcgtttacc
     2281 tttgcactgg tcgcacggca cgtagatatc cggcaggaag tgcatctcca ctttgatcac
     2341 gccatcgccc tgacaggcct cgcagcgtcc gccacgaacg ttaaagctga aacgtcccgg
     2401 cgtatagccg cgcgcacggg attccggtac gcccgcaaac agttcgcgca caggcgtaaa
     2461 cacgccggta taggtcgccg ggttagaacg cggagtacga ccaattgggc tttggtcgat
     2521 atcgatcact ttgtcgaaat gctccagtcc ctgaatatcg cgatacggtg ccggttcggc
     2581 gatggtcgcc ccattcaact ggcgttgggc aatcgggaac agtgtgtcgt taatcagcgt
     2641 cgatttaccg gaacctgaaa cccctgtgat gcaggtaaac agaccgactg gcagcgtcag
     2701 cgtcacgtct ttcaggttat taccgcgtgc gcctgtcagc ttcagcactt tttccggatt
     2761 cgccggaacg cgtttcttcg gcacttcaat cttgcgttta ccgctcatgt actgtccggt
     2821 taacgactcc ggcaccgcca taattgcttc cagtggacct tccgcgacca cttcaccgcc
     2881 gtgaacacct gcgcccgggc caatgtcgat cacatggtca gcggcgcgaa tcgcgtcttc
     2941 gtcgtgctcc accacaatca cggtattacc gagatcgcgc agatggataa gcgtacccaa
     3001 caggcgctcg ttatcgcgct ggtgcaggcc gatagatggc tcgtccagca cgtacataac
     3061 gccaaccagg cccgcaccaa tctggctcgc cagacggata cgctgggctt caccgccgga
     3121 aagcgtttct gccgagcggg aaagcgtcag gtaattcagg ccgacgttaa cgaggaattt
     3181 caaacgatcg ccgatctctt taagaatttt ttccgcaatc ttcgcccgct gacctgcgag
     3241 tttgagattg ttgaagaatt ccatcgcatg accgatgctc atgtcggaga tggcgggcag
     3301 cggcgtattc tcgacataca cgtggcgcgc ttcccgacgc agacgcgttc cttcgcagct
     3361 ggcgcatggg cgattgctga taaacttggc taattcttca cgtaccgcac tggattctgt
     3421 ctctttataa cggcgctcca tattgtgcag cacgccttcg aacggatgac gacggatgga
     3481 ggtatcgcca cgatcgttca tgtatttgaa ttcaatgttt tctttgccag aaccgtacaa
     3541 caccacttta tgcacgttcg cgctcaagct gccccacggc gcttcgacgt cgaacttata
     3601 gtgatctgcc agtgatttca gcatctggaa ataatagaag ttgcggcgat cccagccacg
     3661 gatcgcacca cctgccagcg acagttccgg attctggatc acgcggtcag gatcgaaata
     3721 ttgctgtacg ccaaggccgt cgcaggtcgg gcaggccccc gccgggttgt taaacgaaaa
     3781 cagtcgcggc tccagctcgc gcatactgta accgcaaatt gggcaggcga agttggcgga
     3841 gaacagcagc tcttccgctt tcgggtcgtc catatccgcc actaccgcgg taccaccgga
     3901 aagctctagc gcggtttcaa acgactcggc cagacgttgg gtaagatcgt cacgcacctt
     3961 gaagcgatca accaccactt caatggtatg tttcttttgc agttccagtt tcggcggatc
     4021 ggaaagatcg cagacttcgc catcaatacg agcacggatg taaccctggc ttgccaggtt
     4081 ctccagcgtt ttggtgtgtt cgcctttgcg ctctttaatg attggcgcga gcagcatcag
     4141 acgcttgcct tccggctgcg acagcacgtt atccaccatc tggctgacgg tttgcgccgc
     4201 cagcgggacg tcgtggtccg gacagcgcgg ttcgccgacg cgggcgaaca acaaacgcaa
     4261 atagtcgtgg atttcggtga ttgtccccac cgtagaacgc gggttatgag acgtcgattt
     4321 ctgctcaatt gagatggcag gagaaagccc ctcaatatga tcgacgtccg gcttttccat
     4381 cagtgacaga aactgccgcg cgtaggcgga aagggattca acgtaacggc gctgcccttc
     4441 ggcatataag gtgtcgaaag cgagcgagga tttgccagaa cccgaaagcc cggtcacgac
     4501 aatgagcttg tcgcggggga taacgaggtt gatgtttttg agattatggg tgcgggcgcc
     4561 ccgaacttcg atcttatcca ttcacctttc ccggattaaa cgcttttttg cccggtggca
     4621 tggtgctacc ggcgatcaca aacggttaat tatgacacaa attgacctga atgaatatac
     4681 agtattggaa tgcattaccc ggagtgttgt gtaacaatgt ctggccaggt ttgtttcccg
     4741 gaaccgaggt cacaacatag taaaagcgct attggtaatg gtacaatcgc gcgtttacac
     4801 ttattcagaa cgattttttt caggagacac gaacatggcc agcagaggcg taaacaaggt
     4861 tattctcgtt ggtaatctgg gtcaggaccc ggaagtacgc tacatgccaa atggtggcgc
     4921 agttgccaac attacgctgg ctacttccga atcctggcgt gataaagcga ccggcgagat
     4981 gaaagaacag actgaatggc accgcgttgt gctgttcggc aaactggcag aagtggcgag
     5041 cgaatatctg cgtaaaggtt ctcaggttta tatcgaaggt cagctgcgta cccgtaaatg
     5101 gaccgatcaa tccggtcagg atcgctacac cacagaagtc gtggtgaacg ttggcggcac
     5161 catgcagatg ctgggtggtc gtcagggtgg tggcgctccg gcaggtggca atatcggtgg
     5221 tggtcagccg cagggcggtt ggggtcagcc tcagcagccg cagggtggca atcagttcag
     5281 cggcggcgcg cagtctcgcc cgcagcagtc cgctccggca gcgccgtcta acgagccgcc
     5341 gatggacttt gatgatgaca ttccgttctg atttgtcatt aaaacaatag gttatattgt
     5401 tttaaggtgg atgattaaag catctgccag ccataaaaaa gaagcctccg ttatggaggc
     5461 ttctacgtat caggtcaaaa tcattggcca ttgtggcggt gtctgattca tctcttctat
     5521 catcaccgac tcgatgttgt gccagatagc ggtgatgtcc gccattgtga tgccaagcaa
     5581 ccccattgcg aaccaacagg cggaaacaac gcccagaccg ctgctgatca ccgcaagccc
     5641 gacataatca gaccgacgaa aacggatgtt gagtgtgctg gccagaaaca tcattacggc
     5701 actaagaagt tgccagcgaa gaagaaccac gccagtggtg agggtagcca tcaaacaatt
     5761 cctctgaaaa gagccgatgc cctggacagc gcggcgttgt tcacgggagg cgtggtacac
     5821 tctggctatc gcggggcttg cagaacacaa aaatgaaaca ctctgtttgt ttcattaatt
     5881 ttgtgaacta tatcacaatt gattgtttgt tagccatatt aggccgtgac ttttattgct
     5941 gtacagatta tgtggttttt cagtagcatt aagggcatat cttcccgccg cctctgcatt
     6001 cctgtaggaa attaattttg aatatcaatg aattattttc atccaggtga cgattagaaa
     6061 ggtatcaatt tcaaatcagg caaaagtgct atttataccg taagatttat ctaaagacgt
     6121 cggtacccag ggttttcacc ttgcaatggc cgggtataaa caggcaggaa attgatagca
     6181 atgagtcatc gtgcacgaca ccaattactg gcgttgccgg gcattatctt tttagttctc
     6241 tttcccatca ttctttcgct atggattgcc ttcctttggg caaaatcaga agtgaataat
     6301 cagctccgaa cctttgctca actggcactg gataaatccg agctggtcat tcgccaggca
     6361 gatttagtga gcgatgcagc tgaacgctat caggggcaag tttgcactcc agcccatcaa
     6421 aagcgaatgt tgaatattat tcgtggctat ctttatatta atgaattgat ctatgcccgt
     6481 gataaccatt ttttatgctc atcgctgata gcgcctgtaa acggctatac gattgcaccg
     6541 gccgattata agcgtgaacc taacgtttct atctattatt accgcgatac gccttttttc
     6601 tctggctata aaatgaccta tatgcagcgg ggaaattatg tggcggttat caaccctctc
     6661 ttctggagtg aagtgatgtc tgatgacccg acattgcaat ggggtgtgta tgatacggtg
     6721 acgaaaacct ttttctcgtt aagcaaagag gcctcggcag caacgttttc tccgctgatt
     6781 catttgaagg atttaaccgt acaaagaaat ggctatttat atgcgacagt ttattcgaca
     6841 aaacgcccaa ttgcagccat tgttgcgact tcatatcaac gtcttataac ccatttttat
     6901 aatcatctta tttttgcgtt gcccgccggt attttgggga gtcttgttct gctattactc
     6961 tggctacgta ttcgacaaaa ctatttatct cccaaacgta aattgcaacg cgccctcgaa
     7021 aaacatcaac tttgtcttta ttaccagcca ataatcgata tcaaaacaga aaaatgtatc
     7081 ggcgctgaag cgttgttacg ttggcctggt gagcaggggc aaataatgaa tccggcagag
     7141 tttattccgc tggcagaaaa ggaggggatg atagaacaga taactgatta tgttattgat
     7201 aatgtcttcc gcgatctggg cgattacctg gcaacacatg cagatcgcta tgtttctatt
     7261 aacctgtcgg cctccgattt tcatacgtca cggttgatag cgcgaatcaa tcagaaaaca
     7321 gagcaatacg cggtgcgtcc gcagcaaatt aaatttgaag tgactgagca tgcatttctt
     7381 gatgtcgaaa aaatgacgcc gattattctg gctttccgcc aggcaggtta cgaagtggca
     7441 attgatgatt ttggtattgg ctactctaac ttgcataacc ttaaatcatt gaatgtcgat
     7501 attttgaaaa tcgacaaatc gtttgttgaa acgctgacca cccacaaaac cagtcatttg
     7561 attgcggaac acatcatcga gctggcgcac agcctggggt taaaaacgat cgctgaaggc
     7621 gtcgaaactg aggagcaggt taactggctg cgcaaacgcg gcgtgcgcta ttgccaggga
     7681 tggttctttg cgaaggcgat gccgccgcag gtgtttatgc aatggatgga gcaattaccc
     7741 gcgcgggagt taacgcgcgg gcaataaaac tacaggcggt ggcgataatc gctgggagtg
     7801 cgatcaaact gccgacggaa aacgcgggag aaggtctgct gcgagacata acccaggtcc
     7861 attgcgatat caaaaatcgg acgctcggtg gtgcgcaact caacggcggc cagtaacagg
     7921 cgacgttggc gaatgtaatc gccaagcgtc tgatgcgtca ccgtgcggaa cattcgttgc
     7981 aagtaccact ttgaatagcc tgattttttt gcgactacat caatgttaag cggctggtca
     8041 atatgctcgt caatccatgc gataagatcc tgaataattt tctgatggga cataaatctg
     8101 cctcttttca gtgttcagtt cgttaattca tctgttgggg agtataattc ctcaagttaa
     8161 cttgaggtaa agcgatttat ggaaaagaaa ttaccccgca ttaaagcgct gctaaccccc
     8221 ggcgaagtgg cgaaacgcag cggtgtggcg gtatcggcgc tgcatttcta tgaaagtaaa
     8281 gggttgatta ccagtatccg taacagcggc aatcagcggc gatataaacg tgatgtgttg
     8341 cgatatgttg caattatcaa aattgctcag cgtattggca ttccgctggc gaccattggt
     8401 gaagcgtttg gcgtgttgcc cgaagggcat acgttaagtg cgaaagagtg gaaacagctt
     8461 tcgtcccaat ggcgagaaga gttggatcgg cgcattcata ccttagtggc gctgcgtgac
     8521 gaactggacg gatgtattgg ttgtggctgc ctttcgcgca gtgattgccc gttgcgtaac
     8581 ccgggcgacc gcttaggaga agaaggtacc ggcgcacgct tgctggaaga tgaacaaaac
     8641 taaagcgcca caagggcgct ttagtttgtt ttccggtctt tgtctttctc tctatcccgc
     8701 tggtacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg tggtggaggt
     8761 tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca gtgtaaaagt
     8821 atacttttta accgcaatat ttttgtcatc tcagacgatt ttttatcgca atcctgaacg
     8881 gtatacggct cgataacgct gcaatcttgc gcaccgacga taacgtttgc gcatcaattg
     8941 cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc ggttgaaaaa
     9001 gccgctatga tcgccggata atcgtttgct ttttttacca cccgttttgt atgcgcggag
     9061 ctaaacgttt gcttttttgc gacgcagcaa attgtcgcaa acctggagca ggaagataac
     9121 gtttcgctgg caggggattg tccgccacgc atcttgacga aaattaaact ctcaggggat
     9181 gttttcttat gtctacgcca tcagcgcgta ccggcggttc actcgacgcc tggtttaaaa
     9241 tttcacaacg tggaagcact gtccgtcagg aagtggttgc cgggttaaca acgtttctgg
     9301 cgatggtcta ctcggtcatc gtcgttccag gtatgttggg taaagcaggc ttcccgcctg
     9361 cggcagtttt cgttgcaacc tgtctggttg ccggactcgg ttctatcgtg atgggtctgt
     9421 gggctaatct gccgttggcg attggttgcg ccatctccct gacggcgttt accgcattca
     9481 gcctggtgct ggggcaacat attagcgtac ctgtcgcgct gggtgccgtg ttcctgatgg
     9541 gtgtgctgtt tacggtaatt tctgccacgg gtatccgtag ctggattttg cgcaacttgc
     9601 ctcacggtgt ggcgcacggc acggggattg gtatcggtct gttcctgctg ctcattgccg
     9661 ctaacggtgt cggtctggtg attaaaaacc cgcttgatgg tctgcccgtt gcgctgggtg
     9721 atttcgcgac cttcccggtg attatgtcac tggtaggtct ggcggtgatc atcggcctgg
     9781 aaaaactgaa agtccctggt ggcattctgc tgaccattat cggtatctca attgtcggtt
     9841 tgatcttcga tcctaacgtc catttctccg gcgttttcgc catgccttca ttgagcgatg
     9901 aaaacggcaa ctcactgatt ggcagcctgg acattatggg cgcgctgaat cctgtagtcc
     9961 tgccaagcgt tctggcgctg gtaatgacgg cagtatttga tgccaccgga actatccgtg
    10021 ccgtcgccgg ccaggcgaac ctgctggata aagatgggca aattatcgac ggtgggaaag
    10081 cactgaccac tgactccatg agcagcgttt tctctggcct ggtgggtgca gctccggcag
    10141 cggtatacat cgagtctgcg gcgggtacgg cggcgggcgg taaaaccggt ttgacggcta
    10201 tcaccgttgg cgtgctgttc ctgctgattc tgttcctctc tccgctctct tacctcgttc
    10261 cggggtatgc aacggctccg gcgctgatgt acgttggcct gctgatgctg agcaacgtgg
    10321 cgaaaatcga ctttgctgat tttgttgatg cgatggcggg tctggttacg gcggtattca
    10381 tcgtgctgac ctgtaacatc gtaacaggca tcatgatcgg cttcgcgact ctggtgattg
    10441 gtcgtctggt ttccggcgaa tggcgcaagt tgaacatcgg tacggtcgtt atcgccgtgg
    10501 cgctggtgac cttctatgcg ggtggctggg ctatctaatc tttcatcgga ttctgaaaac
    10561 gggtggcaat ggctgcccgt ttttattttc tccgcacatt gtgtgagctt ttgcgatatt
    10621 ctgaaaaaat gagaattcag gcataacgcc tttctcagat cataaaaaaa catcgcaaac
    10681 agggaacgca tggaaatctt cttcaccata ctgataatga ccctcgtggt ctcgctgtcc
    10741 ggggtggtca ctcgtgtcat gccctttcag atcccgcttc cgcttatgca aatcgccatc
    10801 ggtgcgctac tggcgtggcc gacgtttggt ttgcatgtgg agtttgatcc tgaactcttt
    10861 ttagtcttgt ttatcccgcc gttgctgttc gctgatggct ggaaaacgcc gacccgtgaa
    10921 tttctcgaac atggtcgaga gattttcggc ctcgcgctgg cgctggtggt ggtcaccgtg
    10981 gtcggcattg gcttccttat ttactgggtg gtgccgggca ttccgctgat ccccgccttt
    11041 gcgctggcgg cggtactttc tccgaccgat gctgtggcgc tctccgggat tgttggcgaa
    11101 gggcgcatcc cgaaaaaaat catgggcatt ttgcagggcg aagcgttgat gaatgacgct
    11161 tccggcctgg tgtcgttgaa gtttgccgtg gcagtggcga tggggacgat gatcttcacc
    11221 gtcggcggtg cgacggtcga atttatgaaa gtagccattg gcggtattct tgccggtttt
    11281 gtggtgagct ggctgtatgg tcgctcgctg cgattcctca gccgctgggg cggtgatgaa
    11341 cccgcgacgc agattgttct gctgttcttg ctgccattcg cttcttatct gattgccgaa
    11401 catattggcg tttcgggcat cctcgctgcg gttgccgccg ggatgaccat cacccgctcc
    11461 ggtgtgatgc gccgtgcgcc gctggcaatg cgcctgcgcg caaacagcac ctgggcgatg
    11521 ctggaatttg tctttaacgg catggtgttc ctgctgttag gtctgcagct gccgggtatt
    11581 ctggagacgt cgctgatggc ggcagaaatc gaccctaacg tcgaaatctg gatgctgttt
    11641 accgatatta ttctgatata tgcggcgctg atgctggtcc gtttcggctg gctgtggacg
    11701 atgaaaaagt tcagcaaccg cttcctgaag aagaagccga tggagtttgg ttcgtggacc
    11761 acacgagaaa tcctgatcgc gtctttcgcc ggggtgcgtg gggcgatcac tctggccggt
    11821 gtgctctcta tcccgctgct cttgccggat ggtaacgtct tcccggcgcg ctatgagctg
    11881 gtgttcctgg cggctggcgt cattctcttc tcgctgtttg tcggcgtggt gatgttgcct
    11941 attctgctac aacacattga agtcgcggat cattcgcaac aattgaaaga ggaacgtatt
    12001 gcgcgagcgg caacggcaga agtggcgatt gtggcgatcc agaaaatgga ggagcgtctg
    12061 gcggcggata ccgaagagaa tatcgataac cagctgctta cggaggtcag ttctcgcgtc
    12121 attggtaacc tgcgtcgtcg cgccgatgga cgtaacgacg ttgaaagttc cgtgcaggaa
    12181 gagaaccttg agcgtcgctt ccgtctggcg gcattgcgtt ctgaacgtgc tgaactgtac
    12241 cacctgcgcg ccacgcggga gatcagcaac gaaacgctgc aaaaattact gcacgatctc
    12301 gatttgcttg aagcgttgtt gattgaagaa aatcagtaag ccgaaagcga cgtaaccagg
    12361 cccgaggtaa tctgaaaaag gtgcaatctt ttgcaccttt aacaggttag ctcgggctaa
    12421 cattcatgat tctaaaacaa ataagttatc tctcttttaa agtcatttca ttggctgttc
    12481 ttcgacttaa tctcaatctg cgtgttgcat aatatctgca gtaatattca tagcgactgc
    12541 atgaagatta tcctcagttt gtgacatgcc agtatgagtg gcacaatctt cttcaaagaa
    12601 tttctgtagt gttaccaggg tatcactgtt aatgggaata ttctcctccc cacggagaat
    12661 ggaaatgccc attccctgga cgctagcctc atcgaaatta acccgataga gacgggtatt
    12721 aaacatccag gcttttgtta aaacagaaca gttgaaatgg caattgctta aattggcatt
    12781 attaaaatta acattagcca tattagaatc ggtgcatata accatttcga ggaacgtatt
    12841 tgtcagccgt gtaaaaaaga gtttactatc aattaagttt atattctgga ggattgattt
    12901 gttcaggttg acgcgagaca gatcagcatt ttcaaagtta attaaatcga gtttagagaa
    12961 actaaggtcg gctttaaata aattaacata tggcgctatt actttataaa gatttacctc
    13021 agccatgtat gccatataaa a
//
LOCUS       DAJOSY010000116        12943 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJOSY010000116 DAJOSY010000000
VERSION     DAJOSY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.116"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..772)
                     /gene="paoC"
                     /locus_tag="OHN27_003621"
     CDS             complement(<1..772)
                     /gene="paoC"
                     /locus_tag="OHN27_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HCQ2287996.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAAL"
     gene            complement(769..1725)
                     /gene="paoB"
                     /locus_tag="OHN27_003622"
     CDS             complement(769..1725)
                     /gene="paoB"
                     /locus_tag="OHN27_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HCQ2287997.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            complement(1722..2411)
                     /gene="paoA"
                     /locus_tag="OHN27_003623"
     CDS             complement(1722..2411)
                     /gene="paoA"
                     /locus_tag="OHN27_003623"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HCQ2287998.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTVDEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            2829..3443
                     /locus_tag="OHN27_003624"
     CDS             2829..3443
                     /locus_tag="OHN27_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCQ2287999.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(3691..4020)
                     /locus_tag="OHN27_003625"
     CDS             complement(3691..4020)
                     /locus_tag="OHN27_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCQ2288000.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(4333..5043)
                     /gene="ecpE"
                     /locus_tag="OHN27_003626"
     CDS             complement(4333..5043)
                     /gene="ecpE"
                     /locus_tag="OHN27_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HCQ2288001.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            complement(5012..6655)
                     /gene="ecpD"
                     /locus_tag="OHN27_003627"
     CDS             complement(5012..6655)
                     /gene="ecpD"
                     /locus_tag="OHN27_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HCQ2288002.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            complement(6645..9170)
                     /gene="ecpC"
                     /locus_tag="OHN27_003628"
                     /gene_synonym="yagX"
     CDS             complement(6645..9170)
                     /gene="ecpC"
                     /locus_tag="OHN27_003628"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HCQ2288003.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            complement(9196..9864)
                     /gene="ecpB"
                     /locus_tag="OHN27_003629"
                     /gene_synonym="yagY"
     CDS             complement(9196..9864)
                     /gene="ecpB"
                     /locus_tag="OHN27_003629"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HCQ2288004.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            complement(9922..10557)
                     /gene="ecpA"
                     /locus_tag="OHN27_003630"
                     /gene_synonym="matB"
     CDS             complement(9922..10557)
                     /gene="ecpA"
                     /locus_tag="OHN27_003630"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HCQ2288005.1"
                     /translation="MTHWDITSSIQLGKKCNEKKVLAIALVTVFTGMGVAQAADVTAQ
                     AVATWSATAKKDTTSKLVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKL
                     TSRLITNTLTQLDTSGSTLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYN
                     ASNRTTAQDGFTFSIISGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            complement(10583..11125)
                     /gene="ecpR"
                     /locus_tag="OHN27_003631"
                     /gene_synonym="matA"
     CDS             complement(10583..11125)
                     /gene="ecpR"
                     /locus_tag="OHN27_003631"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HCQ2288006.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLSTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELVYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            11948..12175
                     /locus_tag="OHN27_003632"
     CDS             11948..12175
                     /locus_tag="OHN27_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288007.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(12210..12350)
                     /gene="ykgO"
                     /locus_tag="OHN27_003633"
     CDS             complement(12210..12350)
                     /gene="ykgO"
                     /locus_tag="OHN27_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCQ2288008.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(12350..12613)
                     /locus_tag="OHN27_003634"
     CDS             complement(12350..12613)
                     /locus_tag="OHN27_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCQ2288009.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT         2994 a         3233 c         3323 g         3393 t
ORIGIN      
        1 cgagggccgc cagcagtgca tcgcttctca ggaacagctt gccgccaaac cctccgccga
       61 tatacgggga gataatacgc acattctcca cgggcacttt cagcgttttt gccagatcgg
      121 tgcggcacca gtcaatcatc tgatttgagg tccagagggt aagcttattt ccatcccaga
      181 cggccatcga ggcatgcggc tccatcgcca tatggctctg gtccggggtc gtgtaggtag
      241 catcaatctt caccgcagcg gaggtgaaag ccccgtcaaa gtcaccgacg tttttgtcgg
      301 gcgtgtcttc cggcggctga ctgacggcct gtttctcgtc cgccagggag taagctcctt
      361 tattgcggcg atagtgcgcc tgcaccagcg aggccgccgc tcgcgcctgt tcgaaggtct
      421 cggccactac cagcgcaatg gcctgatgat agtgctcaat agtggggccg cctaacagtc
      481 tggcggtgtt tttgtcgcct ttgccgagtg ccccggcgtt actggcggta atgacagcca
      541 gtacgcccgg cgctttttgc gcggcgtccg tatcaagagc ggtgaggcgt cctttggcaa
      601 tggcggaacc gacgatatag ccataggcgg cgttgggggc ttcttcatgc cattcgtagg
      661 cgtagcgtgc cgtgccggta gttttcagcg gtccgtcgat gcggtcatgg ggacgaccga
      721 caaccttcag ctgatcgatc gggttttccc ctgcgggttt atcaaatttc atgcttgtgc
      781 cctcgcttca gccagtacgg aggcaagcgt tcgcttcgcc aacaggagtt taaaggtgtt
      841 ttcagcggtg ggatgggcgc tggcgaacag cgcgtcatat acggcctgcg ccccctggga
      901 tagctgagca tccgcagcct caatgcgcca gggcttatgt gctactccgc ccagcgcgac
      961 gcgcccgctg ccgtcaggct gaataatcgc cgcgaccgat accagggcaa aggcgtagga
     1021 ggcgcgatcg cgcaccttac ggtagatatg ttttccgccg agcggcggag gtaacgtcac
     1081 cgcaacgata agctcaccgg gaagcagggc ggtttcaatg tgcggcgttt tccccggtgg
     1141 gtgataaaaa tcagccagtg tgatactgcg agtctttccc tccggcgtga tggtttccac
     1201 caccgcatcc agcaaccgca ttgcgaccgc catatcgctc ggatgggtgg caatgcaggc
     1261 ttcgcttacg cctaccaccg cgtgctgacg gctaaagcct tcaagcgccg cgcagccgct
     1321 cccgggcagg cgcttattgc agggctgatt ggtgtcgtaa aaataggggc agcgcgtgcg
     1381 ctggagcaga ttacctgcgg tggttgcctg attacgtaac tgaccagacg cgccagcgag
     1441 cagggcgcgg gagagtaccg cgtaatcacg acgcacgcgc tcgtgagccg ccaggtcggt
     1501 gttccgtacc agtgcgccga tgcgcagccc acccgcgtcg gtcacttcaa tcttatcgag
     1561 cccgagcccg ttcacatcga taaggtgggt gggcgtttca atttccagct tcatcaggtc
     1621 cagcagattg gtcccgcccg cgataaattt tgcgccgggt acgcgctgag cgctaagtgc
     1681 cgcctcggcc ggggtgttca ctcgttcata ggtaaacgcc ttcatgattt tatctccccc
     1741 gcagcatctt caatggcggc aaggatgtta gcgtatgcac cacagcgaca gatgttgccg
     1801 ctcatacgtt cacggatctc atcgacagtt gtttctggag cggaaaccaa atcgaccgtg
     1861 acgtgactgg gaatgccgtc ctgaatctct tttagcaccg ctactgatga gcaaatttgc
     1921 ccggaggtgc agtagccgca ctggaagcca tcatgcttga taaaggccgc ctgcatgggg
     1981 tgaagattat ctggtgaacc caggccttca atggtggtga tctcggcccc ctgatgcatg
     2041 actgcaagcg tcaggcaggc attaagcctg cgaccattga ccagcacggt acaggctccg
     2101 cactgtccgt gatcgcaacc tttcttggta ccgatcaaat gcagattttc acgcaaagcg
     2161 tccagtagcg tggttcgggt atccacctca agctgctcgg ttttgccgtt caccttcagt
     2221 gtcaggggca ttatctctgg cgcgggtgta gctgccggaa cgcttgccgc cagcgtagaa
     2281 tgaggataaa ccacggcggt cgccgctgtt gcggcgctca ctttaatcag atcgcgacgg
     2341 gtcaaactta aatcgtgcgg ctcgtgcttc ccaacccgat tatcttcggg gtattcgcct
     2401 tggttgctca taccaggcct ccggtattct aaaggggaaa ataagagtgc gttgcgagag
     2461 tgctgatgct tttttaaatg ttaagcatag tcggcgacag caggaggatt attcttaaaa
     2521 cgcgaatgca gctatgagcc cggctaataa attcatgttc gccgggatgt tgatgatgat
     2581 gggagctggt ttattgcttt agttgtacga tgcaaaaacc aataaggaaa cctgtgattt
     2641 tcagctctgc atcaccctgc aaatctctgt cacttctaat ataaaaatag ggagaaatga
     2701 tggagcttat attcattggc gattaggaaa ccatcttgtt atacaaaaca atacagttct
     2761 ttacatttgc cttgttttat gaatactcct gaagaggtgt ataacataat ggtacaagca
     2821 gggtagatat gaatatattt gaacaaactc caccgaaccg cagacgttat ggtcttgctg
     2881 cattcattgg gctgattgct ggcgttgttt ccgcattcgt gaagtggggg gctgaagttc
     2941 cattgccgcc acgtagcccg gtggatatgt ttaatgcagc gtgtggcccg gaatcattaa
     3001 tcagggctgc aggccaaatt gattgctcgc gtaattttct caatccaccg tatatttttc
     3061 ttcgagactg gttggggctg acagatccca atgcggctgt ttataccttt gccgggcatg
     3121 tctttaactg ggttggtgtt acgcacatta tcttttcgat agtgtttgct gtcggttatt
     3181 gtgtggtcgc tgaagtattt ccaaaaatta aactctggca gggcttactg gcaggtgctt
     3241 tagcccaact ttttgttcat atgatttcat tccctctcat gggactgacg ccacctctgt
     3301 ttgatctccc gtggtatgag aatgtttctg aaatttttgg acatttagtc tggttctggt
     3361 ctattgaaat tattcgcaga gatttacgaa acagaattac tcatgagcca gaccctgaga
     3421 tccctttagg ctcaaacaga taatgcattg aatgataaaa atggcgcaaa tacagcgcca
     3481 tttttatagg ttaaaaacat tgctttttat attctgatgc agatagtcag tgagtatatc
     3541 gcgctacttc aggatgatgt agatccgaag aacgctacag aagagaggca ttgttgctgg
     3601 caaatagaag aagtatcggg ttttgttacc cctgaaaaac gaagccccgc tattatcgct
     3661 ggcggggcag tgcaattaat tattgtatgt ttataacggt gtcagcccgt atttagccct
     3721 tgcacgattg caagcctcat tgacgactcc gttctcacca gacatggcaa attctctgca
     3781 tgtggacgat cgatttttat atatcgtgca gcaggcattt ttgcccgggg ttcctgcaag
     3841 ggcaatacat cgggggtttt tctgattggt accgctcatg catcggtgaa aaggggatat
     3901 ttgttcagtg agcctggcgg gaatagtacc gccagcatcg tcggcttcgg cccagtaaaa
     3961 agagacgcgg aaaaatgcac aacaggcacc acacgtcatg catggattca gattgctcat
     4021 aattcaccag tacagctaaa aatcgtaaag agaacagcgg tacatcgtat gtaagaacgt
     4081 accgcggttg gctggggaac tttcgatagt gtgagtattg aatgatttcc agccgttacc
     4141 gattttacgt actaattcgt gattaaacca ctcattagca ggctgcctga tacctttccg
     4201 tgtctcctga acactatggt gtgtatatga gaaagcccat cgttacaggt gacattgacg
     4261 atcgcttcca catcatttaa acgatttttg gagctctgag gtggaattct tccctcgacc
     4321 gatggataag tcttagtccg ccgaaggggg cttagccgga caggaatcgc taatcttaat
     4381 gaatttgtcg ttatagacca gataatgatt ccccggctga cgtaactcag gctgatgaac
     4441 cacgtctccc ggacgtagat accaggcatc gccttcctct tcggtcgaat cacatcctgg
     4501 cttaatcagt agcttaaacc atgtgttgcc agtgttactt accgttcctg tcaccttgtc
     4561 gaaggaccat ttaaactgaa cctgacgtgg tcgtactacc agaatggtat ccatcaccac
     4621 caccggctcc gtgctgacct cgccgccggt tgggctacgt cttgtctggt tacgagtggg
     4681 gacttcgcga aatgagaccc ggtagtagcg ctcgcggtta tcctgtggac catgatagta
     4741 aaatttaaaa tactcgctct caccagcctg caacgccagc tggcgggggg cgaaaagcag
     4801 ttcaccatcc accggtcggg tgcgcaattc actgctgccc gggctatcaa tagcactgat
     4861 ggcaatacgg tatatccgtg cgcttttgtt gttattcact acacgtttgc tgacaaagtc
     4921 agtctcggac ggcagcgaaa atgtcagatt gccgaccgaa atcgcctgtg tcaaaggtgc
     4981 cgccagcata cagaccgctg tcagcagggc cttagttaat gttacgccac gtcgcctgaa
     5041 catgaatctc tcctgaagcg ctgacttcgc caaaccagcc gttatcatct accgtacgca
     5101 gcgaaatggc gttatccatt ggaatcgaaa atttgactgt ggtcttatcc atctgcccca
     5161 cttcgccaga gatatcagtc cacggtgtgg tcaaccacag cgcatcggtc atatcccgcc
     5221 agctatcatc gcacccggca tcgtaggttt ttgtagctcc gttgcgggta ataaaggaaa
     5281 gtgtcgctgg gaacggtact ttcgccttac cgtcatcgga gctgaagaca caataggagc
     5341 gcccgccaat cacctgcgcg ggtccggtca ccttgatcag cacttcgtcg gcagctgttt
     5401 taccgctggt ggttacgata tagccgaaat cgagtgccga ctcgccgctg ccaacatatc
     5461 cctcccgcga cggtgtctga gtatattcat cggagataat actgatgctg aaatcacggg
     5521 gtttgataat cagcgtgttg gaggtagaaa attcatacca gccagactcc ggtgatgtgg
     5581 tgttctgaaa gcgaaagttg aatagatctt tggtgttgat atcgctgatc ccgaggttca
     5641 ccatctgctt aaagaagttg ctcgagaaga aaacatagat cgagtctgcg ctcttcatct
     5701 cgttgaaggt gtagtaatag gcggtattgc tcaccggttt ccatgaactg ccattcagac
     5761 tgaactgcat atcgtacgcc ccgacggccg aggctaacga cgagttggcg atcgccggga
     5821 atatatggat tgaggtgttg ctgagtccgt ttgtttgcag ggtatagtta accatcttac
     5881 aacttaatcc tgaacggctg ccgatggttt gcgtccggca gtcggcgttc ccttcgccca
     5941 gagtcggtac tccgtcgctg ttgataaata cttccgccag cgagtgggta tttatcaacc
     6001 gtagatttgc tgctttcgta tgggtgacgt tgcgaacata ccagttgccg gaggcctgat
     6061 ccttacagcg tgcgccgctg ctggcgtcat agttcacaga ggtctggcag gcattgatgg
     6121 tcattgtaaa gctgctgccg acgggcattt gttgcagata ctggtaaaac gcgtctgaca
     6181 acatgccgtg catccacttc gcgccgccgc tggtcaccgt cgcgccataa aatccactgg
     6241 cgtcggtggt ttgcggcagg ataagactgg tggtcatatt acacccagcg taccagttga
     6301 tgcagcgcaa gccagttaaa ggagatgaaa ctggtgaatt ttccagccac atatcaaact
     6361 tccagttggt ataaaggccg gtgttgtaac cgttatcaat gtagccgagg ctttgctgat
     6421 agatggttcc tgaaccagtg tattttaaac cggtccagcg gttggcaccg gtcaggcgcg
     6481 gatccagcgc cccgccagga gtgacgaaaa agttgtcgtc tgagttgttt tcgacaaaca
     6541 caaactcgcg tgccggtgca tccgcccagg ttgttttgct cactgccgct ctgagcgcag
     6601 ttactggcca gattagcgcg aaaattatca tcgttattag taggttaact ctcattctct
     6661 tctcctgtct gcgtcaccgc cgcccacgat gagaggccgc tgcagaccac atcaccgacc
     6721 cagacggcac cgcgcgcctg gttgagttcc agagccactt cgcaggtttt attgccactg
     6781 tagcgaaaat cgatagtggg gtatttctta tccacgtcca tgacaaactc gccgttttca
     6841 tcggttcggg ttcggccgat atggttgtta atccgtgcgt tagccagcag tgtgccgtct
     6901 tccgcacgga tacggccgga gacggtaacc atctgcttca cctctggctc aatgacagcg
     6961 acattacctg gatagagagt cagatggctt ttgcggccgc tgacgatatc gtagctgtcg
     7021 agtgagtttt tgctgttctg taactccacc tcatatcttc catagggaga tagcgggaga
     7081 tagttacgct tgccgttaag cgggaaaatc cgcccgttga tttttgcgct gatctgaccg
     7141 tcgtcctcca gcccggtgtt gaatatcacc ccagcgttgc catcagtccg cccgctggca
     7201 gcgatgtttt taccctgcca gccgacgctg ccattggcgg tcaagttggt attgatgtag
     7261 ccgtccgccg cgctattgac gttcagcgtt ccgctggcgt aacgagcgtc gaactgtgca
     7321 tacgccccac cgctgagggt tttgtcatca ccggtatcgc cggagatggc tcgtgacaga
     7381 ttggcaccaa cagtgcgaat ggtcccttca tcaaactgct tgcgtgctga caggtttgcc
     7441 atggtgtagc cgttttgatg agtcatccct gcgctaaacc agttgcccag tggtagcgag
     7501 agatcgagag cgatatattt ccctgtattg gcgttgctgt cgccgttgtt atagcgctga
     7561 ataccggccc gcaggccaag cgaaccaaag gtaccgctgt agacattttg atagtaatct
     7621 gccgtgtaat aatggctgtt gtaacggcgg tcatcattgt agctgatgct gaatgtgccc
     7681 agcttcgacc acagtgagtt caggttgagt gtgccgccga ttgcacggtt gtcggcatcg
     7741 ctacgtcgca attgattgcc aatgcgggtt ttttcctgat taacccacag cgaactaaag
     7801 cctcccggta gagtggcgct gatgctgccg atgctgctcc atgagctgtc actggccagc
     7861 atattttgca ggttaacgtt gatcgccccc ccaagcggca gcgtcagacg ggtttcaccc
     7921 accgcctgat tatcgtatcc atatcccgtt gccgcccagc taagcgtact caatgagccg
     7981 gaggtcgagg cacctgctag ccaactctct ttagctggtc gcgtcttttt cccgttttcc
     8041 gaccagcgat ccatatgaaa gctaccgccc catacctgcc acgccagtgg tgcaccgacg
     8101 ccgcgccccc ggctaaacag cttattgacc cgctgggtgc gtttgctgat cacgcgaccg
     8161 ttaacgatca cctcaacttc cacatcgtaa atcccgtacg gtagaccccg ggtatccact
     8221 tcatgattgc ccatagtgaa gttctgaacg cttagtagcc gcccatcacg ggtgagatgt
     8281 acttcgcccg ccgccggtaa aaaggcgatc actggcgtgg ctgactggct gctgtcgaag
     8341 atggtggagc tggcctggtt tccccaggaa aggccgtaaa tcttccctgc tgaaatggcg
     8401 gtcatcggcc ctaaggactg caagttccag gtgtcgagca ttccaccggc aaatcggtga
     8461 ccggcaaaat cgcgttcata catcgcttta tataattcac tgtcctgttg accgctaccg
     8521 atcccgtaca gcgagccgtc gagcaccaca tgatgttcgc gcagtgcagt aacgttattc
     8581 agcgacagat agctggatgt gttgctcccg ccgttacgca actggttgtt atagacgccc
     8641 aagttatagc tcagattact gctgagggtg ttaacactgg actgcccgat gtcttcgcta
     8701 cgtgagcgta gtacggtgcc cagcgcttcg cgcttgacca ccagctgcag cagcagttgg
     8761 cgcaagctga gatccagctg cgcgttgtca gtcaggggga tggtaagggc ctcattgaac
     8821 ggggcgttcg ccagagccat cagctgctgt cgagtttgtt cgctgacact ggcgttatct
     8881 tcactctctt ccagctgtat tttccggatg cgtagctgtc catcgtccag ccagataaaa
     8941 gcgctgccga ttcgctgatc gtcctggcga ccctggctac cggcgagatg aatatagagc
     9001 gggacgctca tgccgtcctg aagcgcctga ctgaaggcct gcggaataat caccccacct
     9061 atttgctgcg cacttatgtc agcagcgctg gcatcgggct gaacgaacaa aaagaccatg
     9121 ccgaaggcaa actgggcttt cagtcctggg gagaaccgtc gtaaaggcat tgtcgttatc
     9181 catctgcaca gctatctatt tcacgggaat gaacttatca ccctgccaaa gtgcaacccg
     9241 tcctttgtta tccgcagtgt ccacgcgggt aaaacgacgc gacttgcccg gcatcaggta
     9301 gtaattctct ttacactcct taccgttggc ggctttcagg caaggtccgt aggcgaggat
     9361 ccgcagcgtc gcatttcctg tatttgtcag ggagccgttg gcgtactgaa agtggtagtt
     9421 cgcctgacga ggggcgacga ccagaatggt gccgatgcgg gcggaagcag tggccacagc
     9481 gctgcggttg gcattatcgc gctgcgcatc actgagggcc tgatcaaacc agacaatgcg
     9541 gtagtagcgc tctttttcat ctgcaggtcc cttatagaag aagcggatca cttcgctggc
     9601 ttgggcgggt agcagcaagc tggcgggagt gagtagcaac tcatccggct tgtccattga
     9661 gataacctgc ccgtcgtcaa gcggtgaaga gagccgttcg agacggatat tgataaggcg
     9721 accactgtcg gtactgtttt tgatcgtttt gctcagcgtg ctgctgtcac tgttcataaa
     9781 cgatgatata tcgccgacat ccagcgcctg ggccggagat attccggaaa acagcagagc
     9841 gagaggcaga aggtgctttt tcataacaat tccgtccagg aataaagcag gggggctacc
     9901 cctgctggta catcagagag attaactggt ccaggtcgcg tcgaactgta cgctaacgtc
     9961 gccgctccag atgccttccg gtagagtgct gtaatctgtt actgcggtgg taccattggt
    10021 ggtaccgctg atgatggaga aggtgaaacc atcctgtgcg gtggtacgat tgctggcatt
    10081 gtaaccgtta gccagcgggc taaggttgcc gcccagtacg ccgttggcgg tatcgatcat
    10141 cacggtatcg ccagtttttt cgactgccgc gccgttataa tccacgccca cattcagtgt
    10201 ggaacctgag gtatccaact gggttaatgt gttggtgata agacgtgagg tcagtttaaa
    10261 ggcggtagcc gttgagtcac cctcgatagc cacgtcaaat agacctttct gtgagttaaa
    10321 acctttaatg ccttcggcat actggaacgc caggctaccg agtggcgtca caaccagctt
    10381 actggtggtg tcttttttgg ctgttgccga ccaggtcgct acagcctgag ctgttacgtc
    10441 agcagcctgc gccacaccca tgccggtaaa caccgttacc agagctattg ccagaacctt
    10501 tttttcattg catttcttcc cgagttgaat tgaggacgtg atgtcccagt gagtcatttt
    10561 ttaaaactaa cttgcctgga gtttactgaa ccaacttata tatttttgag tacagcttgg
    10621 cctcagcatt acgccgatgg gtatacactg tcttcacact acaattttca attctggcaa
    10681 tcgatttagg ttgcattccc tgggccgtca tgcggataat ttccatttcc cggtccgtga
    10741 ttttatcttt cttaatatct tttctcagga acctgccatt aatcacatag accagttctt
    10801 ttctaatatc acgactcagt ccagcgtata ccacgcccct aatattgcta ttgtaatacc
    10861 aataatttgc taaggcctct gactttctgg ccgcaatcaa tacgacctgt ttccccttta
    10921 ccgcggataa ccattcatta tcacggttta taaattcaga taaagaatcc gtactgagat
    10981 tgataaacac gaaatctttt ctaattttct ccaacgatag ataaattaat ctgtcgatat
    11041 ccacaatcag ttcagatagt cctttataga agtaggcgtc atggggagac cagatgtatt
    11101 tatcagaacg gttttgacat tccatatggt ttttaacctc atagtcccta ctgtaatcac
    11161 tttgccatgt cactactttc caaacctgta atttttacag gtgcattcag tttccttagt
    11221 aaaccggaag taaataagat acgtagtaca tatcaataaa ggttaaacga gattaagttt
    11281 aattattttc gattgggctt gaatttatgt ttttttcccg atgagtgtca atatgtgaac
    11341 ggaaaaggaa ttcaatggtt aagtcaatat ttagaaaaac tgaaaatatt gcttttttga
    11401 taagaagaaa aagacatttg gtttcataag gttattggca atataacgtt caattcttac
    11461 ttgcatttta tattggtcag gaactttgcc ctgaagtgtt aggaatagtc ttaatgcgct
    11521 gtgtgatgga ctagtaaaaa aacaagtttt aagggtaagg gatagaatat aaaactatga
    11581 tgagctgtca ttgaagatga tttgccaatc agtaagatat tttaatggat taacatgccg
    11641 cacaaaatgg ttacaggtgt tcattccaat aagtaatgga aaattgaacg ggcgtatgta
    11701 cgtcatctgt tcggtaagcc cccaagagcc agattaagtg aagttaaacg aaggctgcca
    11761 gctgtcacag gaacgatggc ctttttgtca gggcactgca ttcagttatc tgtgcgcgtt
    11821 actctaactg gcactcgaac gtcggcgacg gggttaccgg tggatcagac tgttcctgtg
    11881 gcgtaaacgt ttttatgtta gtaccaacag atttaccaca tttatcatct taacagtttg
    11941 agtgaaaatg caggctgcgc cgcaaaggga ggagactgag tggcgtgttc agtctaaaag
    12001 agggttgatg ccagcataca ggggcgaagc tggtcaacaa gtcaacatta agataatgga
    12061 atattcggag agaaacgtca ggcagttggc ctcaaatgaa caggaagaat atatccccag
    12121 gaaaattaat gttggtgtta taaacacacc aacattaatc cgatctgatt attaatcaga
    12181 caaatatcgt taatccgtcg aattttgaat caacgttttt tcttacgacc ctgaacggcc
    12241 ttaaaacgtg gattagattt acaaatcaca tatagccgtc cttttcgctt cacaatctga
    12301 cagtctggat ggcgttcttt tgcggtacgc agagagttaa gaactttcat cacgccccct
    12361 ttttcgtgct aacaaaacga ccaaaacgtt gggtgaatcg tgcaacattt ccttctgatg
    12421 ccactgttct cagcttccct gtatagaacg ggtgcgattt agaagagaca tcaattgtca
    12481 cgtatggata cgttacgcca tccagctcaa tctcacggtc tgttttgata gtcgagccga
    12541 ttttaaagta ctcatcaaca ctggtgtcgt ggaacaccac agtacgatac tcaggatgga
    12601 tattgggctt catcattttt acctgttatg ttataacata accataaaga tacatgctca
    12661 tccggatcat ttcaacactc aatccctgtg actctttgtg gatgtggaat tttcgcaacg
    12721 gggaactctg gcattgttag cagccattgg tgacgctccc ataaacgcct ggatgagtga
    12781 ttgatgatgg ttttgctgag agtatggatt tttcgagaat ggaggttatc tccactttac
    12841 tggccgatga tttatcgcag tatgattgat tacctgacag cgtgtattcc tctcccacgg
    12901 atataataat tacttacaaa aaagggagag gatgcatatt tta
//
LOCUS       DAJOSY010000117        12836 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJOSY010000117 DAJOSY010000000
VERSION     DAJOSY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.117"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            147..1256
                     /gene="livK"
                     /locus_tag="OHN27_003635"
     CDS             147..1256
                     /gene="livK"
                     /locus_tag="OHN27_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCQ2288010.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1304..2230
                     /gene="livH"
                     /locus_tag="OHN27_003636"
     CDS             1304..2230
                     /gene="livH"
                     /locus_tag="OHN27_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCQ2288011.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2227..3504
                     /gene="livM"
                     /locus_tag="OHN27_003637"
     CDS             2227..3504
                     /gene="livM"
                     /locus_tag="OHN27_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCQ2288012.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3501..4268
                     /gene="livG"
                     /locus_tag="OHN27_003638"
     CDS             3501..4268
                     /gene="livG"
                     /locus_tag="OHN27_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCQ2288013.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4270..4983
                     /gene="livF"
                     /locus_tag="OHN27_003639"
     CDS             4270..4983
                     /gene="livF"
                     /locus_tag="OHN27_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCQ2288014.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5380..6696
                     /gene="ugpB"
                     /locus_tag="OHN27_003640"
     CDS             5380..6696
                     /gene="ugpB"
                     /locus_tag="OHN27_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCQ2288015.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFRKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6794..7681
                     /gene="ugpA"
                     /locus_tag="OHN27_003641"
     CDS             6794..7681
                     /gene="ugpA"
                     /locus_tag="OHN27_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCQ2288016.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7678..8523
                     /gene="ugpE"
                     /locus_tag="OHN27_003642"
     CDS             7678..8523
                     /gene="ugpE"
                     /locus_tag="OHN27_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCQ2288017.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8525..9595
                     /gene="ugpC"
                     /locus_tag="OHN27_003643"
     CDS             8525..9595
                     /gene="ugpC"
                     /locus_tag="OHN27_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCQ2288018.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPMVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9592..10335
                     /gene="ugpQ"
                     /locus_tag="OHN27_003644"
     CDS             9592..10335
                     /gene="ugpQ"
                     /locus_tag="OHN27_003644"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCQ2288019.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10322..10762)
                     /locus_tag="OHN27_003645"
     CDS             complement(10322..10762)
                     /locus_tag="OHN27_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCQ2288020.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10882..12624
                     /gene="ggt"
                     /locus_tag="OHN27_003646"
     CDS             10882..12624
                     /gene="ggt"
                     /locus_tag="OHN27_003646"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCQ2288021.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADNLKTYGSEVLPNHENSKAIFLKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGLNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12662..>12836)
                     /locus_tag="OHN27_003647"
     CDS             complement(12662..>12836)
                     /locus_tag="OHN27_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288022.1"
                     /translation="FEAREFLETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYL
                     QDYEIKKATFGLP"
BASE COUNT         2827 a         3294 c         3694 g         3021 t
ORIGIN      
        1 agggatttta atcactatta tcacaaaata ctgcgctaaa cccttaatca gacaggcaaa
       61 aacagtgcag tataaaaaaa gaacagtctg atttgttaac acataaaaac aaagcaacac
      121 aacatcacga atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga
      181 tgattgcact ggcaatttca cacaccgcta tggctgacga tattaaagtc gccgttgtcg
      241 gcgcgatgtc cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg
      301 cgattaaaga cattaatgcc aaagggggaa ttaagggcga caagctggtt ggcgtggaat
      361 atgacgacgc ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaatgacg
      421 gcattaaata cgttattggt catctgtgtt cttcttctac ccaacctgca tcagatatct
      481 acgaagacga aggtattctg atgatctcgc cgggagcgac caacccggag ctgacccaac
      541 gcggttatca acacattatg cgtactgccg ggctggactc ttcccagggg ccaacggcgg
      601 caaaatacat tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac
      661 agtatggcga agggctggcg cgttcggtgc aggacgggct gaaagcggct aacgccaacg
      721 tcgtcttctt cgacggtatt accgccgggg agaaagattt ctccgcgctg atcgcccgcc
      781 tgaaaaaaga aaacatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga
      841 tgctgcgcca ggcccgttcc gttggcctga aaactcagtt tatggggccg gaaggtgtgg
      901 gtaacgcatc attgtcgaat attgccggtg atgctgccga aggcatgttg gtcactatgc
      961 caaaacgcta tgaccaggat ccggcaaacc agggcatcgt tgatgcgctg aaagcagaca
     1021 agaaagatcc gtccgggcct tatgtctgga tcacctacgc ggcggtgcaa tctctggcga
     1081 ctgcccttga gcgtactggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta
     1141 acggtgcaaa caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat
     1201 ttgattttgg tgtcttccag tggcacgccg acggttcatc cacggcagcc aagtgatcat
     1261 cccaccgccc gtaaaatgcg ggcgggttta gaaaggttac cttatgtctg agcagttttt
     1321 gtatttcttg cagcagatgt ttaacggcgt cacgctgggc agtacctacg cgctgatagc
     1381 catcggctac accatggttt acggcattat cggcatgatc aacttcgccc acggcgaggt
     1441 ttatatgatt ggcagctacg tctcatttat gatcatcgcc gcgctgatga tgatgggcat
     1501 tgataccggc tggctgctgg tggcggcggg attcgtcggc gcaatcgtca ttgccagcgc
     1561 ctacggctgg agtatcgaac gggtggctta ccgcccggtg cgtaattcta agcgcctgat
     1621 tgcgcttatt tccgcaatcg gtatgtccat cttcctgcaa aactacgtca gcctgaccga
     1681 aggttcgcgc gatgtggcgc tgcccagcct gtttaacggt cagtgggtgg tgggccatag
     1741 cgaaaacttc tctgcctcta ttaccaccat gcaggcggtg atctggattg tcaccttcct
     1801 cgccatgctg gcgctgacga ttttcattcg ctattcccgt atggggcgcg cctgtcgtgc
     1861 ctgcgcggaa gatctgaaaa tggcgagcct gcttggcatc aacaccgacc gggtgattgc
     1921 tttgaccttt gttattggcg cggcgatggc ggcagtggct ggcgtactgc tcggtcagtt
     1981 ctatggcgtc attaacccct atatcggctt tatggccggg atgaaagcct ttaccgctgc
     2041 ggtgctcggt gggattggca gcattccagg ggcgatgatt ggcgggctga tcttggggat
     2101 tgcggaggcg ctctcttctg cctatctgag tacggaatat aaggatgtgg tttcatttgc
     2161 cctgctgatt ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga
     2221 gaaagtatga aaccgatgca tattgcaatg gcgttgctct ctgccgcgat gttttttgtc
     2281 ctggcgggcg tctttatggg cgtacaactg gagctggatg gcaccaaact ggtggtcgac
     2341 acggcttcag acatccgttg gcagtgggtg tttatcggca cggcggtggt ctttttcttt
     2401 cagcttttgc gacctgcttt ccagaaaggg ctgaaaagcg tttccggacc gaagtttatt
     2461 ctgcctgcaa ttgatggctc cacggtgaag caaaagctgt tcctcgtggc gctgctggtg
     2521 cttgcggtgg cgtggccgtt tatggtgtcg cgcgggacgg tggatatcgc caccctgacc
     2581 atgatctaca ttattctcgg tctcgggctg aacgtggttg ttggtctttc tggtctgctg
     2641 gtgctggggt acggcggctt ttacgccatc ggcgcttaca cctttgcgct gctcaatcac
     2701 tattacggct tgggcttctg gacttgtctg ccgattgccg ggttaatggc agcggcggcg
     2761 ggcttcctgc tcggctttcc ggtgctgcgt ctgcgtggtg attatctggc gatcgttacc
     2821 cttggcttcg gcgaaattgt gcgcatattg ctgctcaata acaccgaaat taccggcggc
     2881 ccgaacggaa tcagtcagat cccgaaaccg actttcttcg gccttgagtt cagccgtacc
     2941 gctcgtgaag gcggctggga cacgttcagt aatttctttg gcctgaaata cgatccctcc
     3001 gatcgcgtca tcttcctgta tctggtggcg ttgctgctgg tggtgctaag cctgtttgtc
     3061 attaaccgcc tgctgcggat gccgctgggg cgtgcgtggg aagcattgcg tgaagatgaa
     3121 attgcctgcc gttcgctggg cttaagcccg cgtcgtatca agctgaccgc ctttaccatc
     3181 agcgccgcgt ttgccggttt tgccggaacg ctgtttgcgg cgcgtcaggg ctttgtcagc
     3241 ccggaatcct tcacctttgc cgaatcggcg tttgtgctgg cgatagtggt gctcggcggt
     3301 atgggctcgc aatttgcggt gattctggcg gcaattttgc tggtggtgtc gcgcgaattg
     3361 atgcgtgatt tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg
     3421 atctggcgtc cgcagggctt gctgcccatg acgcgcccgc aactgaagct gaaaaacggc
     3481 gcagcgaaag gagagcaggc atgagtcagc cattattatc tgttaacggt ctgatgatgc
     3541 gcttcggcgg cctgctggcg gtgaacaacg tcaatcttga actgtacccg caggaaatcg
     3601 tctcgttaat cggccctaac ggtgccggaa aaaccacggt ttttaactgc ctgaccggtt
     3661 tctacaaacc caccggcggc accattttac tgcgcgatca gcatcttgaa ggtttaccgg
     3721 ggcagcaaat tgcccgcatg ggcgtggtgc gcaccttcca gcatgtgcgt ctgttccgtg
     3781 aaatgacggt aattgaaaac ctgctggtgg cgcagcatca gcaactgaaa accgggctgt
     3841 tctctggcct gttgaaaacg ccttctttcc gtcgcgccca gagcgaagcg ctcgaccgcg
     3901 ccgccacctg gcttgagcgc attggtttgc tggaacacgc caaccgtcag gcgagtaacc
     3961 tggcctatgg tgaccaacgc cgtctggaga ttgcccgctg catggtgacg cagccggaga
     4021 ttttaatgct cgacgaaccg gcggcaggtc ttaacccaaa agaaaccaaa gagctggatg
     4081 agctgattgc cgaactgcgc aatcatcaca acaccactat cttgttgatt gaacacgata
     4141 tgaagctggt gatggggatt tcagaccgaa tttatgtggt caatcagggg acgccactgg
     4201 caaacggtac gccggagcaa atccgtaata acccggacgt gatccgtgcc tatttaggtg
     4261 aggcataaga tggaaaaagt catgttgtcc tttgacaaag tcagcgccca ctacggcaaa
     4321 atccaggcgc tgcatgaggt gagcttgcat atcaatcagg gcgagattgt cacgctgatt
     4381 ggcgcgaacg gggcggggaa aaccaccttg cttggtacgt tatgcggcga tccgcgcgcc
     4441 accagcgggc gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc
     4501 atgcgcgaag cggtagcgat tgtcccggaa gggcgtcgcg tcttctcgcg gatgacggtg
     4561 gaagagaacc tggcgatggg cggcttcttt gccgaacgtg accagtttca ggagcgcata
     4621 aagtgggtgt atgaactgtt tccacgtctg catgagcgcc gtgttcagcg ggcgggcacc
     4681 atgtccggcg gtgaacagca gatgctggcg attggtcgcg cgctgatgag caatccgcgt
     4741 ttgctgctac ttgatgagcc atcgctcggt cttgcgccga ttatcatcca gcaaattttc
     4801 gacaccatcg agcagctgcg cgagcagggg atgactatct tcctcgtcga gcagaacgcc
     4861 aaccaggcgc taaagctggc ggatcgcggc tacgtgctgg aaaacggcca tgtggtgctc
     4921 tccgataccg gcgacgcgct gctggcgaac gaagcagtga gaagtgcgta tttaggcggg
     4981 taataacacg ttgattgata aggagtcaaa agactccttt gagacaggtg acaaatgtaa
     5041 aattgcctga tgcgctgcgc ttatcaggcc tactgggtga gttgcaatat attgaatttg
     5101 cacgatcttg taggccggat aaggcgttta cgccgcatct ggcatgaaac gatgagtaat
     5161 ctgtagagtt tgattcagac cttctatttt tccgcttatc cgtgccccat ctcccatttt
     5221 ccctcaccca cgccgtcacc gccttgtcat ctttctgaca ccttactatc ttacaaatgt
     5281 aacaaaaaag ttatttttct gtaattcgag catgtcatgt taccccgcga gcataaaacg
     5341 cgtgaattcg cgcattcggt acaacaagag agataaacga tgaaaccgtt acattataca
     5401 gcttcagcac tggcgctcgg actggcgtta atggggaatg cacaggcagt gacgaccatt
     5461 ccgttctggc attctatgga aggggaactg ggtaaagagg tggattctct ggcccaacgt
     5521 tttaacgccg aaaatccgga ttacaaaatt gtaccgacct ataaaggcaa ctacgaacag
     5581 aatttaagcg cggggattgc cgcatttcgt accggcaacg ctccggctat tttgcaggtt
     5641 tatgaagttg gcaccgccac catgatggca tcgaaagcca ttaaaccggt atatgacgtg
     5701 tttaaagagg cggggattca attcgatgag tcgcagtttg tgccgacggt ttccggttac
     5761 tactccgaca gcaaaacggg ccacttactc tcccagccat tcaacagctc gacccccgtt
     5821 ctctattaca acaaagacgc cttcaggaaa gcaggattag acccggaaca accgccgaaa
     5881 acctggcagg atctggcgga ctatgccgca aaactgaaag cctccggcat gaagtgcggc
     5941 tacgccagcg gctggcaggg ctggatccaa ctggaaaact ttagcgcctg gaacggtctg
     6001 ccgtttgcca gcaaaaacaa cggctttgac ggcacggacg cggtgctgga gttcaacaag
     6061 ccggagcagg tgaaacacat cgccatgctc gaagagatga acaagaaggg cgacttcagc
     6121 tacgtcggtc gtaaggatga atccaccgag aagttctata acggtgattg cgcgatgacc
     6181 accgcctctt ccggttctct tgccaacatt cgcgagtacg ccaaattcaa ctacggcgta
     6241 ggcatgatgc cttacgacgc cgatgcgaaa gatgcgccac aaaacgccat tatcggcgga
     6301 gccagcctgt gggtgatgca gggtaaagat aaagaaacgt ataccggtgt ggcgaagttc
     6361 ctcgatttcc tcgcgaagcc agaaaacgct gccgagtggc atcagaaaac cggttatctg
     6421 ccaatcacca aagcagcgta tgacctgacc cgtgagcagg gcttttatga gaaaaaccca
     6481 ggggcggata ccgcgacgcg tcagatgctg aataagccgc cgttgccgtt caccaaaggg
     6541 ctgcgtctgg gcaacatgcc gcagatccgc gtgattgtgg atgaagagct ggagagcgtg
     6601 tggaccggta agaagacacc acagcaggca ctggataccg ccgttgagcg tggaaatcag
     6661 ttgctgcgcc gctttgagaa atcgacgaag tcttaatcag tgtaatgtcg gatgcgtttc
     6721 gcttatctga cctggcatcg cgtgtaggcc ggataagcga agcgcatccg gcacagttca
     6781 ggaattaacc gtaatgtcat catcccgtcc ggtgttccgc tcgcgctggc tgccttatct
     6841 gctggtcgcg ccgcagctca tcatcaccgt tatctttttt atctggcctg cgggcgaagc
     6901 gttgtggtac tcgctacaaa gcgtcgatcc gtttggtttc tccagccagt ttgtcggcct
     6961 ggataacttc gtcacgctgt tccatgacag ctactatctc gactccttct ggacgacgat
     7021 aaaattcagc acctttgtca ccgtcagcgg tttgctggta tcgctgttct ttgcggcgct
     7081 ggtggagtac atcgtgcgcg gcagccgttt ctatcaaacc ttaatgttgc tgccttacgc
     7141 cgtggctccc gccgttgccg ccgtattgtg gatcttcctg tttaaccccg gtcgcgggct
     7201 gatcacccat tttctcgcgg agttcggcta cgactggaac cacgcgcaaa acagcggtca
     7261 ggcaatgttt ctggtggtgt ttgcctcagt atggaagcaa atcagctaca acttcctgtt
     7321 cttctatgcc gcgctgcaat ccattccccg ttcgttgatc gaagccgcag ccatcgacgg
     7381 tgcagggccg attcgccgct tctttaagat tgcgctgccg cttatcgccc cggtgagttt
     7441 cttcctgctg gtagtgaacc tggtgtatgc cttcttcgac accttcccgg tgatcgacgc
     7501 cgccacgtcc ggcgggccgg ttcaggccac cacgacgctg atttataaga tctaccgcga
     7561 aggttttacc ggactggatc tggcttcgtc tgccgcacag tcggtggtgt tgatgttcct
     7621 cgtcatcgtg ctgacggtgg tgcagttccg ctatgttgaa agcaaggtgc gttaccaatg
     7681 attgagaacc gtccgtggct gacgatattc agccatacca tgctgatcct cgggatcgcg
     7741 gtgatcctct tcccgctgta cgtggcgttt gtcgcggcga cgctggataa acaggccgtc
     7801 tatgccgcgc cgatgacgct catccccggc acacatctgc tggaaaacat ccacaacatc
     7861 tgggtgaacg gggtaggcac gaatagcgcg ccgttctggc ggatgttgct taacagcttt
     7921 gtgatggcgt tcagcattac gctcggcaaa attaccgtct cgatgctctc ggcatttgcc
     7981 attgtctggt ttcgttttcc gctacgtaac ctcttcttct ggatgatttt tatcaccctg
     8041 atgctgccgg ttgaagtacg tatcttcccg acggtggaag tcatcgccaa cctgcagatg
     8101 ctcgacagct acgccggttt aacgctgccg ctgatggcct cggcgaccgc tactttcctg
     8161 ttccgccagt tctttatgac gctgccggat gagctggtgg aagccgcgcg gatcgacggc
     8221 gcatcgccaa tgcgcttctt ttgcgacatc gtttttccgc tctccaaaac taatctggcg
     8281 gcgctgtttg tgatcacctt tatctacggc tggaatcagt atttgtggcc gttgttgatt
     8341 attaccgatg tggatctcgg caccaccgtg gcagggatca aagggatgat cgctacaggc
     8401 gaaggcacca cggaatggaa ctcagtgatg gcggcgatgt tgttaacgct tatccctccg
     8461 gtggtgattg ttttagtgat gcagcgtgcc ttcgtgcgcg gcctggtcga tagtgagaaa
     8521 taagatggca ggactgaaat tacaggcagt aaccaaaagc tgggatggta aaacccaggt
     8581 cattaaaccg ctgacccttg atgtggcgga tggcgaattt atcgtgatgg tcgggccgtc
     8641 tggctgcggg aaatcgacgc tgctgcgcat ggttgccggg ctggagcggg tgacagaagg
     8701 cgatatctgg atcaacgacc agcgcgtgac tgaaatggag ccaaaagatc gcgggattgc
     8761 gatggtgttc cagaactacg cgctttatcc gcatatgagt gtcgaagaaa acatggcgtg
     8821 ggggctgaaa attcgcggca tgggcaagca gcaaattgcc gagcgcgtta aagaagcggc
     8881 gcgcattctg gagctggacg gtctgctcaa acgtcgcccg cgcgagcttt ccggcggtca
     8941 gcgccagcgt gtggcgatgg gccgcgcgat tgtgcgcgat ccggcggtgt tcctgtttga
     9001 tgagccgctc tctaacctcg atgccaagct gcgcgtgcag atgcgtcttg aactgcaaca
     9061 gttgcaccgt cgcctgaaaa cgacttcact ctacgttact cacgatcagg ttgaagcgat
     9121 gacgctcgcc cagcgagtaa tggtgatgaa cggcggtgtt gccgaacaga ttggcacacc
     9181 agttgaagtc tacgaaaagc ccgccagcct gtttgtagcg agttttatcg gcagtccggc
     9241 gatgaacctg ctgacaggcc gcgtgaataa cgaaggcacg catttcgaac tggacggcgg
     9301 tattgagctg ccgctaaacg gtggctaccg tcagtatgcc gggcgtaaaa tgactctcgg
     9361 cattcgcccg gaacatattg cgctaagctc gcaggcagaa ggcggcgtac cgatggtgat
     9421 ggacacgctg gagatcctcg gcgcagataa cctggcgcac ggacgctggg gcgagcagaa
     9481 gctggtggtg cgactggcgc atcaggagcg cccgacggca ggcagcacgc tgtggctgca
     9541 tctggcggaa aatcagctgc atctttttga tggtgaaaca ggacaacgag tatgagtaac
     9601 tggccttatc cccgcatcgt cgctcatcgt ggcggcggca agctggcccc ggaaaacacc
     9661 ctggcggcaa tcgacgtcgg ggcaaaatac ggtcataaga tgatcgaatt tgacgcgaag
     9721 ttatcgaaag atggcgagat cttcctgctc catgacgaca atctcgaacg caccagcaac
     9781 ggctggggcg tcgcgggtga actgaactgg caggatttac tgcgcgtgga tgcgggcagt
     9841 tggtacagca aagcgtttaa aggtgagccg ctgccgttgc tttcgcaggt ggcggaacgc
     9901 tgtcgcgaac acgggatgat ggcgaatatc gaaatcaaac ccaccactgg cactggacca
     9961 ttaacgggca aaatggtggc gctggcggca cgccaactgt gggccggtat gacgccgccg
    10021 ctgctgtcat cgtttgagat tgatgcttta gaagctgcac agctggcggc accggaactg
    10081 ccgcgtggtt tattgcttga tgagtggcgc gacgactggc gcgaactgac cgcgcggctg
    10141 ggctgcgtct ctattcatct caatcataag ttactcgata aagtgcgagt gatgcagttg
    10201 aaagacgccg gactgcggat tctggtttat accgtcaata aaccccagcg cgcggcagag
    10261 ttgctgcgct ggggcgtgga ttgcatctgt accgatgcga ttgacgtgat tggtccgaac
    10321 tttacggccc aatagttttc aacggaatgt caggctgcgg cgtgctgggc tgattcagca
    10381 tgtcgccgtt tctcctctcc ggcagcatat gctgctgatt caacgaacta tccggattac
    10441 ggttgctgtt taacatcccg ccgttggtgt tgggcagcat ttgctgccgg gcgggatttc
    10501 gctcccccgg ctgtgactgc aacacccgct gagaattatt gtttatctgg ttttctaaat
    10561 gctgctgttg caactgagtt tgcgttttca gttgctgatt cagcatccct ttttgctgga
    10621 tttgctgagt ctgcatctgg gtttgcatcc gctgctggct gggtatctga taccccggct
    10681 ggttagggtt gttcagagta ttaatgggct gtgcaaagcc gacaaacggc aggagtgccg
    10741 taagaatcag aagtcgtttc atcgcgtatc ctcctctgaa gatatccttt aagtttactc
    10801 gcttcccgac aaaacgatga ttaattcaga gttatatacc aggcttagct ggggttgccc
    10861 cttaatctct ggagaataac gatgataaaa ccgacgtttt tacgccgggt ggccattgct
    10921 gctctgctct caggaagttg ttttagcgcc gccgccgcgc ctcctgcgcc gcccgtctcg
    10981 tatggtgtgg aggaagatgt cttccacccg gtacgcgcga aacagggaat ggtagcgtct
    11041 gtggacgcca ctgccactca ggtgggggtg gatatcctca aggagggcgg gaatgccgtt
    11101 gatgccgccg tggcggtggg ctacgcgctg gcggtaacgc atccgcaggc agggaatctg
    11161 ggcggtggtg gttttatgtt aatccgctcg aaaaatggca ataccacggc tatcgatttc
    11221 cgcgaaatgg cacccgccaa agcgacccgc gatatgttcc tcgatgatca gggcaacccg
    11281 gacagcaaaa aatcactcac ttcgcatctg gcttccggca caccgggtac ggtagcaggt
    11341 ttctcgctgg cgctggataa atacggcacc atgccgctga acaaagtcgt gcagcccgcg
    11401 tttaaactgg cacgcgatgg ttttatcgtt aacgacgcgc tggctgacaa tctcaaaacc
    11461 tacggtagcg aagtgttgcc gaatcacgaa aacagtaaag ctatcttctt gaaagagggc
    11521 gagccgctga aaaagggcga cacgctggtg caggcgaacc tggcaaagag cctggagatg
    11581 attgctgaaa acggcccgga cgaattctat aaaggcacga ttgcggaaca gatcgcccag
    11641 gagatgcaga aaaacggtgg cttgatcact aaagaagatt tagcggccta taaagcggtc
    11701 gaacgcactc cgataagcgg cgattatcgc gggtatcagg tttactccat gccaccgcca
    11761 tcctccggcg ggatccatat cgtacaaatc ctcaatattc tggaaaactt cgatatgaag
    11821 aaatacggct ttggcagcgc cgatgcgatg caaatcatgg cagaagcgga gaaatacgcc
    11881 tacgccgacc gctcggaata tcttggcgac ccggattttg tcaaagtacc gtggcaggcg
    11941 ctgaccaata aagcctatgc caaatctatt gccgatcaaa ttgatatcaa taaagcgaag
    12001 ccatccagcg aaattcgccc cggcaagctt gcgccttatg agagtaatca aactacccat
    12061 tactcagtgg tggataaaga tggtaacgcg gtggcggtga cctatacgct gaacaccacc
    12121 ttcggtacgg gcattgtcgc gggcgagagc ggtattctgc ttaataacca gatggatgat
    12181 ttctccgcca aaccgggcgt accgaacgtt tacgggctgg tgggcggtga tgccaacgcc
    12241 gtcgggccga acaaacgccc gctgtcgtcg atgtcgccga ccattgtggt gaaagacggt
    12301 aaaacctggc tggttaccgg tagcccaggc ggtagccgga tcatcactac agtgctgcaa
    12361 atggtggtga atagcatcga ttatggcttg aacgtcgccg aagcgaccaa tgcgccgcgt
    12421 ttccaccatc agtggttgcc ggacgagctg cgtgtcgaaa aagggtttag cccggatacg
    12481 ctcaagctgc tggaagcaaa aggtcagaaa gtggcgctga aagaggcgat gggcagtaca
    12541 caaagcatta tggttgggcc ggacggtgag ttgtacggcg catccgaccc gcgctcggtg
    12601 gatgatttaa cggcggggta ctaaggttag cggccctctt cgtgggaaga gggctatttt
    12661 gtcagggcaa gccgaaggta gcctttttta tttcgtaatc ctgtagatat tcttccagcg
    12721 gttttgccgt tcccagagaa tgaatttcac cactgtcttt atcaataata aaaggtgcgt
    12781 taccagctaa gcgcgcggcc tcatctccag tttcgagaaa ttctcgtgct tcgaaa
//
LOCUS       DAJOSY010000118        12789 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJOSY010000118 DAJOSY010000000
VERSION     DAJOSY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.118"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(124..4347)
                     /gene="rpoC"
                     /locus_tag="OHN27_003648"
     CDS             complement(124..4347)
                     /gene="rpoC"
                     /locus_tag="OHN27_003648"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCQ2288023.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(4424..8452)
                     /gene="rpoB"
                     /locus_tag="OHN27_003649"
     CDS             complement(4424..8452)
                     /gene="rpoB"
                     /locus_tag="OHN27_003649"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCQ2288024.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            complement(8772..9137)
                     /gene="rplL"
                     /locus_tag="OHN27_003650"
     CDS             complement(8772..9137)
                     /gene="rplL"
                     /locus_tag="OHN27_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCQ2288025.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            complement(9204..9701)
                     /gene="rplJ"
                     /locus_tag="OHN27_003651"
     CDS             complement(9204..9701)
                     /gene="rplJ"
                     /locus_tag="OHN27_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCQ2288026.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            complement(10114..10818)
                     /gene="rplA"
                     /locus_tag="OHN27_003652"
     CDS             complement(10114..10818)
                     /gene="rplA"
                     /locus_tag="OHN27_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCQ2288027.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            complement(10822..11250)
                     /gene="rplK"
                     /locus_tag="OHN27_003653"
     CDS             complement(10822..11250)
                     /gene="rplK"
                     /locus_tag="OHN27_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCQ2288028.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            complement(11409..11954)
                     /gene="nusG"
                     /locus_tag="OHN27_003654"
     CDS             complement(11409..11954)
                     /gene="nusG"
                     /locus_tag="OHN27_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCQ2288029.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            complement(11956..12339)
                     /gene="secE"
                     /locus_tag="OHN27_003655"
     CDS             complement(11956..12339)
                     /gene="secE"
                     /locus_tag="OHN27_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCQ2288030.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            complement(12569..>12789)
                     /gene="tuf"
                     /locus_tag="OHN27_003656"
     CDS             complement(12569..>12789)
                     /gene="tuf"
                     /locus_tag="OHN27_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2288031.1"
                     /translation="FFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGRTVGAGVVAKVLS"
BASE COUNT         2951 a         3493 c         3170 g         3175 t
ORIGIN      
        1 atctctacat tgattatgag tattcagaaa ttccttaaat attctgacaa atgctctttc
       61 cctaaactcc ccccataaaa aaacccgccg aagcgggttt ttacgttatt tgcggattaa
      121 cgattactcg ttatcagaac cgcccagacc tgcgttcagc agttctgcca ggctggcaga
      181 tgcgtcttct gcagtcacct gcggtgcagc cggagcttca cccgcagcac ggcgacgcat
      241 acgatcctgg tggtacgcgt aaccggtacc tgcagggatc agacgaccca cgataacgtt
      301 ctctttcagg ccgcgcagtt cgtcgcgttt gcccgcaacg gctgcttcgg tcagcacgcg
      361 agtggtctcc tggaacgatg ccgcggagat gaaggactcg gttgccagag acgctttggt
      421 gatacccagc agatcgcggg agtaagttgc acccactttg ccgttcgctt ccagttcgcg
      481 gtttgcgatc ttgacgcgag agtattcaac ctgttcgcct tccaggaagt cggagctgcc
      541 cgcgttaacg atggtagctt tacgcagcat ctgacgaacg ataacttcga tgtgtttatc
      601 gttaatctta acgccctgca gacggtatac gtcctgtact tcgttaacga tgtaacgagt
      661 cacagcatga acaccacgca gacgcagaat gtcgtgcggc gcttccggac cgtcggaaat
      721 tacgtcacca cgttctacac gttcaccttc gaacacgttg agctgacgcc atttcggaat
      781 catctcttcg tacggatcgc taccgtctac cggggtgata accagacgac gtttaccttt
      841 ggtttcttta ccgaaggaaa cgataccgct gatttcagcc aggattgccg gctctttcgg
      901 acgacgtgct tcgaacaggt ccgcaacgcg cggcagacca ccggtgatgt ccttggtacc
      961 gccggattcc tgcggaatac gcgccagggt gtcaccagag ctgatctgta cgccatcttc
     1021 cagctgaaca atcgctttac ccggcaggaa gtactgcgca ggcatatcgg tacccgggat
     1081 cagaacgtcg ttaccctgag catcaacgat tttcagtgcc ggacgcagat ctttaccacc
     1141 tgcggtacgt tctgcggaat ccagaaccac cagcgaagac agaccggtca gttcgtcggt
     1201 ctgacgcgta atggtctggc cgtcgatcat gtcagtaaag cgtacaaaac cgcttacttc
     1261 ggtgataacc ggcatggtgt gcgggtccca gtttgcaacg gtttcgccgc cagcaacctg
     1321 ttcgccatcg cctttcgcca gtaccgcacc gtaaggtact ttgtagcttt ctttagtacg
     1381 accgaattcg tcgatcagtt tcagttcggt gttacgggaa gtgataacca gtttaccgct
     1441 ggagttcaca accgacttca cgttgctgag cttgatgcta cctttgtttt tcacctggat
     1501 gctggattca gcagccgcac gagatgccgc accaccgatg tggaacgtac gcatggtcag
     1561 ctgtgtaccc ggttcaccga tggactgtgc cgcgataaca ccgattgctt cacccttgtt
     1621 gatgatgtgg ccacgcgcca ggtcacgacc gtagcagtgc gcacatacac caaagtcggt
     1681 gtcacaagat acaacagaac gtactttaac cgcgtcgaca gagttctctt ccagcaggtc
     1741 acaccactgt tcgtgcagca gcgtgttgcg cggaacgagg atatcagcag tacccggctt
     1801 cagaacgtct tcagcagtta cacgacccag tacgcgatcg cgcagcggct ctttaacgtc
     1861 accaccctcg ataaccggag tcatcatgat accttcatgg gtaccacaat cgtcttcggt
     1921 aaccaccagg tcctgcgcca cgtcaaccag acgacgagtc aggtaaccgg agttcgcagt
     1981 tttcagtgcg gtatccgcca gacctttacg agcaccgtgg gtggagatga agtactggag
     2041 tacgttcaga ccttcacgga agttcgcggt gattggcgtt tcgatgatgg agccatccgg
     2101 cttcgccatc agaccacgca taccagcaag ctgacgaatc tgtgccgcag aaccacgcgc
     2161 accggagtcg gccatcatgt agatgctgtt gaaggaaacc tgcttctctt cctgaccgtc
     2221 acggttaata acggtttcag tttgcaggtt atccatcatc gctttggata cacgatcgtt
     2281 cgccgcagcc cagatatcga taactttgtt gtagcgttcg cccgcagtta ccagaccaga
     2341 ctggaactgc tcctgaattt cagcaacttc tgcttctgcc tcggagatga tttcgtgttt
     2401 cttctccggg atgaccatgt catcgatacc aacagatgca ccagaacgcg ctgcataggc
     2461 aaagccggtg tacatgatct ggtccgcaaa aataacggtc ggtttcagac cgagaatgcg
     2521 gtagcaggtg ttcagcattt tggagattgc ttttttaccc agcgcctggt tgacgatgga
     2581 gtaaggcaga cctttcggta caatcatcca cagaatggca cggccaacag tcgtgtcttt
     2641 caggctagtt ttcgctacta attcaccgtt agcatctttt tcatactcgg tgatacgcac
     2701 tttaacgcgc gcatgcagag aagccagacc agagcgatac agacgttctg cttctttcgg
     2761 gccagtcagc accatgcctt cgcctttggc gttaacacag tcacgggtca tgtagtacag
     2821 acccagtaca acgtcctgag acggaacgat gattggttcg ccgttcgccg gggacaggat
     2881 gttgttggta gacatcatca gcgcacgcgc ttccagctgg gcttccagcg tcagcggtac
     2941 gtgaacagcc atctggtcac catcgaagtc ggcgttatat gccgcacaaa ccagcgggtg
     3001 cagctggata gctttacctt cgatcagtac cggttcaaat gcctggatac ccagacggtg
     3061 cagagtcggt gcacggttca gcagtactgg gtgttcgcgg ataacttcgt ccaggatatc
     3121 ccaaacgaca gcttcttcgc gctcaaccat tttcttcgca gctttaatgg tggtagcaag
     3181 accacgcagt tccagcttgc cgtagatgaa cggtttgaac agctccagcg ccattttctt
     3241 cggcagaccg cactgatgca gacgcaggta tggacccacg gtgattacag aacgaccgga
     3301 gtagtcaaca cgcttaccga gtaggttctg acggaaacga ccctgtttac ctttgatcat
     3361 gtcggccaaa gatttcagag gacgcttgtt agaaccggtg attgcacgac cgcgacgacc
     3421 gttatccagc agggcgtcta ccgcttcctg cagcatacgt ttttcgttac gtacgatgat
     3481 atccggcgca gccagatcca gcagacgttt cagacggttg ttacggttaa tgacgcgacg
     3541 atatagatcg ttcaggtcag aagtcgcgaa acgaccacca tccagcggaa ccagcggacg
     3601 cagatctggc ggcagtaccg gcagaacggt caggatcatc cactctggtt tgttaccaga
     3661 ctgaacgaac gcttccagca gtttgatacg cttggtcagc tttttacgct tagtttcgga
     3721 gttggtttcg ttcagctctt cacgcagctg ttcgcactct tgctccagat ccatgctctt
     3781 cagcagagcc tggattgctt ccgcacccat tttcgcgtcg aattcgtcac cgaactcttc
     3841 cagcgcgtcc agatactgct cttcagtcag gatctgctga cgttccaggt tggtcatacc
     3901 gccttcgata accacatagg attcgaagta cagtacgcgt tcaatatcgc gcagcggcat
     3961 atcgagcagc aggccgatac gggacggcag cgatttcagg aaccagatgt gcgcagtcgg
     4021 ggaagccagt tcgatgtggc ccatacgctc acggcgtact ttggtctggg tcacttcaac
     4081 gccgcacttc tcacagatga cgccacggtg tttcaggcgc ttgtacttac cgcacaggca
     4141 ctcgtaatct tttaccggcc caaagatacg ggcgcagaaa aggccgtcac gttctggttt
     4201 gaacgtacgg tagttgatgg tttccggctt tttaacttca ccgaaagacc atgaacggat
     4261 catgtctggc gaagccagag caattttgat cgcatcaaac tcttcggttt tagtctgcgc
     4321 tttcagaaac tttaataaat ctttcacgga tttgctcccg tcggagttag cacaatccgc
     4381 tgccgggttt taccccgaca gcagtgacct gtttgagcga gaattactcg tcttccagtt
     4441 cgatgttgat acccagcgaa cgaatctctt tcaacaatac gttgaaggat tctggcatgc
     4501 ccggctccat ctgatggttg ccgtccacga tgtttttata catcttagta cgaccgttca
     4561 cgtcatcaga cttaacggtg agcatttcct gcagggtgta tgctgcgccg tatgcttcca
     4621 gcgcccacac ttccatctcc ccgaaacgct gaccaccgaa ctgtgcctta ccacccagcg
     4681 gctgctgagt aaccaggctg taagaaccgg tggaacgcgc gtgcatcttg tcgtcgacca
     4741 ggtggttcag tttcagcatg tacatgtaac caacggttac cggacgttcg aactgttcac
     4801 cagtgcggcc gtcgtacagg cggatctgac cagaagtcgg caggtcgcca agtttcagca
     4861 gctctttaat ttctgcttct ttcgcaccgt cgaacaccgg cgttgcgatt ggcatacctt
     4921 tgcgcaggtt ttcagccaga cgcataactt cttcatcgct gaaggtactc aggtcaactt
     4981 tctgacgaac gtcagcgccc agatcgtacg cacgctggat gaattcgcgc agtttcgcga
     5041 cttcctgctg ctgtttcagc atggcgttga tcttgtcgcc gatacctttc gcagccatac
     5101 ccaggtgggt ttcgaggatc tgaccgatgt tcatacgaga cggtacgccc agcgggttca
     5161 gtacgatgtc taccggcgta ccgttttcat cgtaaggcat atcttcgatc gggttgatct
     5221 tagaaattac acccttgtta ccgtgacgac ctgccatctt gtcaccaggc tggatacggc
     5281 gtttaaccgc cagatatacc ttaacaatct tcagcacgcc cggtgccaga tcgtcgccct
     5341 gggtgatttt gcggcgtttc gcttcgagtt tcttctcgaa ctcgtgtttc agttcgtcat
     5401 actgctcagc cagctgttcc agctgatttt gtttctcttc gtcggtcagg cccagctcca
     5461 gccagcgatc gcgcggcagt ttgtcgagct tctcagcttc aacgccaccg gctaccagca
     5521 cagcacggat acggctgaac agacccgctt cgaggatctg cagttcttca gacaggtctt
     5581 tcttcgcctg tttgagctgc atttcttcga tttccagcgc acgtttgtct ttttctacgc
     5641 catcgcgagt aaagacctga acgtcgataa ccgtaccgga tacaccgttt ggtacgcgca
     5701 gagaagagtc tttaacgtca gaggctttct caccgaagat cgcacgcagc agtttttctt
     5761 ctggggtcag ctgagtttca cctttcggcg taaccttacc aaccagaatg tcgccaccgg
     5821 tcacttccgc accaatataa acgataccgg attcatccag tttggagagc gcagcttcac
     5881 ccacgttcgg gatgtcagcg gtgatctctt ctggccccag cttggtgtca cgggacacac
     5941 acgccagttc ctgaatgtgg atggtggtga aacggtcttc ctgaacaaca cgctcggata
     6001 cgaggatgga gtcttcgaag ttgtaaccat tccacggcat gaacgctacg cgcatgttct
     6061 gaccaagcgc cagttcaccg aggtcggtgg acggaccgtc tgccagcacg tcgccacgtt
     6121 caaccggttc acccagagac acacacggca tctggttgat acaggtgttc tggttagaac
     6181 gggtgtattt ggtcaggttg tagatgtcga tacctgcttc acccggatac atctcgtctt
     6241 cgttaacttt gataacgata cgggaagcat ccacgtactg aacgacacca ccacgtttag
     6301 ctaccgcagt tacaccggag tcaacggcaa cagcacgttc cataccagta ccaaccagcg
     6361 gcttatcagc acgcagagtc ggaacggcct gacgttgcat gttcgcaccc atcaatgcac
     6421 ggttggcgtc atcgtgttcc aagaacggga tcagggacgc accgacggat accacctgct
     6481 gggtggatac gtccatgtag tcaacctggt cacggctgaa caagctggat tcgcctttgc
     6541 tacggcaagt taccaggtct tctacgaagt ggccttcttc atccaggttg gagttcgcct
     6601 gggcgataac gtagttgcct tcttcgatag cagacaggta gtgaatttcg tcagttacaa
     6661 caccgtcggt cactttacga tacggagtct caaggaagcc gtattcgtta gtctgtgcgt
     6721 acacggacag agagttgatc agaccgatgt tcggaccttc aggggtttcg attggacata
     6781 cgcgaccgta gtgagtcggg tgtacgtctc gaacttcgaa gcctgcacgt tcacgggtca
     6841 gaccgcctgg gccgagtgcg gagatacgac gtttgtgcgt aatctcagac agcgggttgt
     6901 tctggtccat aaactgagac agctggctgg aaccgaagaa ctctttcact gctgcggaaa
     6961 tcggcttggc gttgatcata tcctgaggca tcagggtatc cagatcgccc agagacagac
     7021 gctctttcac cgcacgctct acacgtacca ggccaacgcg gaactggttt tccgccattt
     7081 cgccaacgga acggatacga cggttgccga ggtggtcgat atcatcgact tcgcctttac
     7141 cgttacggat atcgatgagc tttttcataa catcaatgat gtcgtctttg ctcaggatac
     7201 cggaaccttc gatttcttcg cgcagcagag aacggttgaa cttcatacga ccaaccgcag
     7261 acaggtcata acggtcttcg gagaagaaca ggttctcgaa caggctttcc gctgcttcac
     7321 gagtcggcgg ctcgccaggg cgcatcatgc ggtagatttc taccagtgcg ctcagacggt
     7381 cgttagttgg gtcgacacgt aaggtttcag agatatatgg gccgtgatcc agatcgttgg
     7441 tgaacagcgt ttcgatacgc ttgtgaccag actggctcag cttagccagc agatccaggc
     7501 tcagctccat gttcgctgcg cagatcagct cgccggtaga ctcatcaata tagtctttag
     7561 caaccacttt acctgcgatg tactcaaccg ggacttcgat cagtttgacg tcgtcttttt
     7621 ccagctggcg aatgtggcgc gcagtgatac ggcggccttt ttctacgtac actttaccgt
     7681 tagcttcgat gtcaaaagat gcggtttcac cacgcaggcg ttccggcacc agttccatct
     7741 gcagcttgtt atcacggatt tcaaagataa ctttttcaaa gaacaggtcg aggatctgct
     7801 ctgtggtgta gttcagggcg cgcagaatga tggtcgcagg cagtttacgg cgacggtcga
     7861 tacgtacgaa caggttgtcc ttcggatcga attcgaagtc cagccaggaa ccacggtaag
     7921 ggatgatgcg cgcgttatac agcactttac ccgaagagtg ggttttacct ttgtcggagt
     7981 caaagaagac gcccggacta cggtgcagct gggaaacgat aacacgctca gtaccgttga
     8041 taacaaaggt accgttgtct gtcatgagcg gaatttcgcc catgtagact tcttgttctt
     8101 taatgtcttt tacggtgcct tccggcgctt cgcgctcata gatcaccaga cgcagtttaa
     8161 cgcgcagcgg tgcggaatag gtcacgccac ggatttgaca ttcctggacg tcaaacaccg
     8221 gttcgccaag gcggtagctg acgtattgca gctcggaatt accgctgtag ctctgaatcg
     8281 ggaatacgga acggaaagca gcttccagac catactgccc ttcaggatct tgctcgataa
     8341 atttctgaaa cgagtcaagc tggatagaaa ggagataagg tacatccaga acttgtggac
     8401 gtttaccaaa atccttacga atacgttttt tctcggtata ggagtaaacc atagggttcc
     8461 tcagctcgct gacaagtcga cccatctgtc cattgagcgg acagtttgtg caacactatt
     8521 ttgttgaccg gaaaatggaa cactttccgc aatgcctgtt gctatcacgc ttaaaccatt
     8581 tcattgcgat ttacacagaa cggacgtcct gtcgcagtat attaagtcgt cgatagaaac
     8641 aagcattgaa aggcacagca gtagtcaaac agtgtgaaac gctactggcg ccttacagcg
     8701 caaaaaggct ggtgactaaa aagtcaccag ccatcagcct gatttctcag gctgcaaccg
     8761 gaagggttgg cttatttaac ttcaacttca gcgccagctt cttccagagc ttttttcagt
     8821 gcttctgcgt cgtctttgct cacgccttct ttcagagcag ccggtgcaga ttctaccagg
     8881 tctttagctt ctttcagacc caggccagtt gcgccacgta ctgctttgat aacagcaact
     8941 ttgttagcgc cagcagcttt cagaattacg tcgaattcag ttttttcttc agcagcttca
     9001 accgggccag cagctacagc tacagcagca gcagcggaaa caccgaattt ttcttccatt
     9061 gcagagatca gttctacaac gtccattaca gacatagctg caactgcttc aatgatttga
     9121 tctttagtga tagacattta aattgttcct gaatatcaga ataagtttat acgtaagcga
     9181 atgcgttaaa aagataactg cgattaagca gcttctttcg catcgcgtac agcagccaga
     9241 gtacgaacca gtttgccagc cgaagcttct ttcatggttg ccatcaggcg tgcaattgct
     9301 tcttcgtagg tcggcagagt tgccaggcgg tcgatctgag acgccgggat cagctcacct
     9361 tcaaaggcag cggctttgac ctcaaatttt gcattcgctt tcgcgaactc tttgaacaga
     9421 cgagcagcag cgcccgggtg ttccatagag tatgcaatca gggtcggacc aacaaacgcg
     9481 tctttcaggc actcgaacgg agtaccttca acagcacggc gcagcagggt gttacgaaca
     9541 acacgcatgt atacgccagc ttcgcgacct gctttacgca gttcggtcat tttatctaca
     9601 gttacgccac gggaatccgc aactactgca gacagcgcgc ctttggctac ttcgctgact
     9661 tcagcaacaa tcgcttgttt gtcttgaaga tttaaagcca ttagctttgc tcctggatgt
     9721 ttgccagaga aaatctctgg aactcacttc actcctccaa acggagagag cgtcttaatt
     9781 acggtgagca gaaacaagcc agaatataaa agaataatct tagcgttctg tcaccgtcta
     9841 cgcaggggat taagtttctt gcgaaactcc tgcggtcttc gacggaggcc tggataggcc
     9901 aggctccaac gaacaaatct tttctatccg tcgctattca gcttgtgacg gcgttggctt
     9961 ccttcgttcg caccagtcac atagttagct atgctcctgg cgactcactc agttgccgcc
    10021 ttgccacaac ccgaatatct cggatatacg tctgcattaa gcattatacg tgggggtaag
    10081 attgtagaca aaatcaccgc ccacgtaaag gcattagttt acagaagcgc tcaggccagc
    10141 ctggtcaact gcaacacctg cacccatggt ggtggagatg ctaactttct tgatgtacac
    10201 gcctttcgcc tgagtcggtt ttgctttttt cagcgcaacc agcagagctt ccaggttttc
    10261 tttcagtttg tcagcgtcaa agtccacttt accgatggtg gtgtggatga tgccgttttt
    10321 gtcgttacgg taacgaacct ggccagcttt agcgttttta accgcttcag caacgttcgg
    10381 tgttacagta cccactttcg ggtttggcat caggccgcgc ggacccagaa cctggcccag
    10441 ctggccaaca acgcgcattg catccggaga agcaataaca acgtcaaagt tcatttcgcc
    10501 tttcttgatc tggtcagcca gatcttccat acctaccagt tcagcacctg cagctttagc
    10561 agcttcagcg tttgcaccct gggtaaatac ggctacgcga acggaacggc cagtaccgtg
    10621 cggcagtaca gttgcaccac gtacgttctg gtcagattta cgagcgtcga tgccgaggtt
    10681 aacagctacg tccacgcttt ctacgaattt agcagtcgcc agctctttca gcagtgcgat
    10741 agcttcgttg atgtcgtact gtttggttgc atcaactttc tcgcggataa cacgcatgcg
    10801 cttggtcagt ttagccattt cttagtcctc cactaccagg cccatggaac gtgcagtacc
    10861 ttcgatggag cgagtcatcg cttcaatgtc ggcaccagtc atgtcggcag ctttggtctg
    10921 cgcgatttcc tgcagctgag cgcgggaaat tttacccact ttgtctttgt tcggcttacc
    10981 ggaaccagac ttgataccag ccgctttttt cagcagaact gctgccggcg gagtcttggt
    11041 aacgaaagtg aaagaacggt cagcgtaaac ggtgattact accggaatcg gcagaccttt
    11101 ttcgatggaa tcagtttttg cgttgaacgc tttgcagaat tccatgatgt ttacgccctg
    11161 ctgacccaga gccggaccta ctggcggact cgggttagcc ataccagctg caacctgcag
    11221 cttgacatag gcttgtactt tcttagccat tataaattcc tcaagttggg taataacgcc
    11281 tctgagaggc tccccgtgat ataaaaaatc gttttacggg caaggcccat aaaaacaaaa
    11341 ggcgcgaaat tgtattccaa tctcgcgcct tgtgcaacga ttaaatcgcc gcttttttga
    11401 tcgctgggtt aggctttttc aacctggctg aagtccagct ctaccggggt cgcacgaccg
    11461 aagatagaaa cagacacttt cagacgagat ttctcgtaat ccacttcttc aacaacaccg
    11521 ttgaagtcag cgaacggacc atcattaaca cggaccattt cacccggttc aaacagcgtt
    11581 ttcggacgcg gcttatcacc aacctgctgc aggcggttca taatcgcatc gacttcttta
    11641 tcgctgattg gcgcaggacg atcggaagta ccgccgatga agcccatcac acgcggtacg
    11701 ctgcgcacca ggtgccagct cgcgtcgttc atcaccatct gaacgaggac gtagccaggg
    11761 aagaatttac gttcgctttt gcgacgctga ccgccacgga tttcaaccac ttcttcggtt
    11821 ggtaccatga cttcaccaaa caaatcttcc atgttgtgta atttgatatg ctcacgtagc
    11881 gacgttgcta cgcggccttc aaaaccggaa aacgcctgaa cgacgtacca gcgcttttta
    11941 ggagcttcag acatctcaga acctcaggcc agtgataaag gataccaggc gaaccagaat
    12001 accatccagt ccccacagga tcagtgacat tactgcggta accgcagcca caatcagcgt
    12061 ggtgtgcaat gtttcctggc gagtcggcca aatgacctta cggacttcgg tacgcgcttc
    12121 acgggcaaaa gcaacggtag ctttaccttt tgtcgttaac agcgcgacac cacccgctgc
    12181 agcaatcaga attactacgg ccagcgcacg cagcggcagc ataatgtcgc gataaaggta
    12241 gttgccgaca atcgccacca ggagcaatgc caccacaacg acccacttca tcgcttccag
    12301 gccgcgcccg cttccttgag cttcggtatt cgcactcata aaccaacctg tcagaagttt
    12361 tctacaaaca ttttcacccc gcgatcgcga ggcaaaccaa atcgaaacgc gtattagctt
    12421 ttcggattat acgccctcaa cagagcctgt ctcagcaatg attatgacaa agaaaatcac
    12481 tgatgagcca ggttctggta cgaaagcgtg caaaaagggc atcaaatgat gcccttttag
    12541 tgcgcattgc gtcaaatgtt atcggcaatt agctcagaac ttttgctaca acgcccgcgc
    12601 caacggtacg gccgccttca cggattgcga aacgcagacc gtcgtccatc gcgatcgggt
    12661 ggatcagggt aacaaccatt ttgatgttgt cgcccggcat taccatctct acgccttccg
    12721 gcagttcgat ggtaccagtc acgtcagtag tacggaagta gaactgcgga cggtagcctt
    12781 tgaagaacg
//
LOCUS       DAJOSY010000119        12663 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJOSY010000119 DAJOSY010000000
VERSION     DAJOSY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.119"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..221
                     /locus_tag="OHN27_003657"
     CDS             <1..221
                     /locus_tag="OHN27_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCQ2288032.1"
                     /translation="LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLL
                     EDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            591..1415
                     /gene="gadX"
                     /locus_tag="OHN27_003658"
     CDS             591..1415
                     /gene="gadX"
                     /locus_tag="OHN27_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCQ2288033.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAAGIRL"
     gene            1783..2511
                     /gene="gadW"
                     /locus_tag="OHN27_003659"
     CDS             1783..2511
                     /gene="gadW"
                     /locus_tag="OHN27_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCQ2288034.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2874..5987)
                     /locus_tag="OHN27_003660"
     CDS             complement(2874..5987)
                     /locus_tag="OHN27_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCQ2288035.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6012..7169)
                     /gene="mdtE"
                     /locus_tag="OHN27_003661"
     CDS             complement(6012..7169)
                     /gene="mdtE"
                     /locus_tag="OHN27_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCQ2288036.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVHLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7229..7507
                     /locus_tag="OHN27_003662"
     CDS             7229..7507
                     /locus_tag="OHN27_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288037.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPVSI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLIS"
     gene            complement(7508..8035)
                     /gene="gadE"
                     /locus_tag="OHN27_003663"
     CDS             complement(7508..8035)
                     /gene="gadE"
                     /locus_tag="OHN27_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCQ2288038.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDADRIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8834..9406)
                     /gene="hdeD"
                     /locus_tag="OHN27_003664"
     CDS             complement(8834..9406)
                     /gene="hdeD"
                     /locus_tag="OHN27_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCQ2288039.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9661..9993
                     /gene="hdeA"
                     /locus_tag="OHN27_003665"
     CDS             9661..9993
                     /gene="hdeA"
                     /locus_tag="OHN27_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCQ2288040.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10110..10436
                     /gene="hdeB"
                     /locus_tag="OHN27_003666"
     CDS             10110..10436
                     /gene="hdeB"
                     /locus_tag="OHN27_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCQ2288041.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10500..11147
                     /locus_tag="OHN27_003667"
     CDS             10500..11147
                     /locus_tag="OHN27_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCQ2288042.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYNLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11189..11719)
                     /locus_tag="OHN27_003668"
     CDS             complement(11189..11719)
                     /locus_tag="OHN27_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2288043.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11875..12441)
                     /gene="slp"
                     /locus_tag="OHN27_003669"
     CDS             complement(11875..12441)
                     /gene="slp"
                     /locus_tag="OHN27_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCQ2288044.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3493 a         2821 c         2866 g         3483 t
ORIGIN      
        1 acctctctga acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact ctcggcggtg
       61 aagccaccga catcgtggtg atgcgcatta tgtgtcgtcg cggcttcgaa atggactttg
      121 ctgaactgtt gctggaagac tacaaagcct ccctgaaata tctcagcgat cacccgaaac
      181 tgcagggtat tgcccagcag aacagcttta aacacacctg ataacataac gttgtaaaaa
      241 ccgaatgccc agcctttaaa aaaacagctg ggcattcggt tgcttattaa tcgcaataat
      301 atattggctg tctattcatc gtgttgataa gatatatgtt atatgaatgt ttattaaatg
      361 tagcgatttc ctttaatgtc acgaaaaggg attatttgct tgctattaat ttcactgcgt
      421 tcttaaacac gtttttgtat tataaatcca gtcatcctgc ccgtagaaca tcacacatta
      481 tcatcctgtt ctcccgcttc gtttaaattt atttatcaat caatttgact taagagggcg
      541 gcgtgctaca ttaattaaca gtaatatgtt tatgtaatat taagtcaact atgcaatcat
      601 tacatgggaa ttgtctaatt gcgtacgcaa gacataaata tattctcacc atggttaatg
      661 gtgaatatcg ctattttaat ggcggtgacc tggtttttgc ggatgcaagc caaattcgag
      721 tagataagtg tgttgaaaat tttgtattag tatcaaggga tacgctttca ttatttctgc
      781 cgatgctcaa ggaggaggca ttaaatcttc atgcacataa aaaaatttct tcattactcg
      841 ttcatcactg tagcagagat attcctgttt ttcaggaagt tgcgcaacta tcgcagaata
      901 agaatcttcg ctatgcagaa atgctacgta aaagagcatt aatctttgcg ttgttatctg
      961 tttttcttga ggatgagcac tttataccgc tgcttttgaa cgttttgcaa ccgaacatgc
     1021 gaacacgagt ttgtacggtt atcaataata atatcgccca tgagtggaca ctagcccgaa
     1081 tcgccagcga gctgttgatg agtccaagcc tgttaaagaa aaaattgcgc gaagaagaga
     1141 catcatattc acagttgctt actgagtgta gaatgcaacg tgctttgcaa cttattgtta
     1201 tacatggctt ttcaattaag cgagtcgcag tgtcctgtgg atatcacagc gtgtcgtatt
     1261 tcatttacgt ctttcgaaat tattatggga tgacgcccac agagtatcag gagcgatcgg
     1321 cgcagggatt gccgaaccgt gactcggcgg caagtattgt tgcgcaagga aatttttacg
     1381 gcactgaccg ttctgcggca ggaataagat tatagaattt tactcagaca taaaaaaaac
     1441 ccggcatagg ggaccgggaa gaggatagtc tgccgtctcc agactaataa accgttataa
     1501 cactccctgt tggcacggga aactttgtgc tctcagtaag ttaaatataa cttttactgg
     1561 aaataagatc agccattttt ttataaacat aagctatacg ctgtgcgaaa atatattcgt
     1621 gctgcattta cttattatca attaactgtt atgcaaaact actttgtgga taaattttgg
     1681 tcctaccaaa tctggcagtt tttgcgctaa gaaacagtct ggcaacattt cattagtata
     1741 ctgaaattga aataatcgca gtatgaaata taagggataa tcatgactca tgtctgctcg
     1801 gtgatcctca ttcgtcgttc attcgatatt tatcatgaac agcataaaat atcgctgcat
     1861 aacgagagta tcgtgctgct ggagaaaaat ttggcagacg attttgcgtt ttgttcaccg
     1921 gatacgcgac gactggatat cgatgagctg acagtttgcc attacttaca aaatattcgt
     1981 cagctaccac gcaatttagg gttacatagc aaagaccgtc tattaattaa ccagtcaccc
     2041 cccatgccgc tggtgacggc gatttttgat agcttcaatg aatccggggt aaattcaccg
     2101 atactgagca atatgctcta cctttcctgt ttatcgatgt tttctcataa gaaagaactg
     2161 atccccttac ttttcaatag catcagcact gtttcaggaa aagttgaacg ccttattagc
     2221 tttgatatcg ccaaacgttg gtatctgcgc gatatcgcgg aaagaatgta taccagcgag
     2281 agtctcatca aaaaaaagtt gcaggatgaa aatacctgtt tcagtaaaat attactcgct
     2341 tccaggatgt cgatggccag acgattactc gagttacgtc aaattcctct gcatactatt
     2401 gcagaaaaat gtggctatag cagtacgtcg tactttataa acacatttcg acaatattat
     2461 ggtgtaacgc cacatcagtt tgcgcaacat tcgccaggta ccttttcctg acatattttg
     2521 catttgaata ttggtcagga tctcacacct gcttcatgtg aaactcttcc ctgatgattt
     2581 ctgccgggct accggctagt tctctttcgc agtttgccag gcggtatagg tattttcagg
     2641 aggaaaagac tcggcatgtt tgggattatt aagctgacaa ttcatacgat tatgggaatg
     2701 tgggtcagta ttgtgctgtt caaattgatg acgaatggct ggagtggttt ctactttcag
     2761 tgctgtgtac taagcctggt attcctgacg gttagctggt tgttgagtgg tgaatggtta
     2821 gcaggaaaga gtaaggctga accttcacgt tcaaccttac tctcatttac acgttacgct
     2881 tttttaaagc gggcaaagag atgttccact acaacgaaaa agaccggaac gaagtaaatt
     2941 gccagcactg ttgcggcaaa catcccgccc atcacgccgg tacctaccgc gttttgcgcg
     3001 ccagaaccgg caccatgact gataaccagc ggcagcacgc cgagaataaa ggccagagag
     3061 gtcatcagga ttgggcgtaa acgcatccgc gccgcttcga tgattgcctc tatcggcgtt
     3121 ttcccttctt tctgcatcat ctcaacggca aattcgacaa tcaggatggc gttcttggcg
     3181 gaaagcccga tggtggtcag caaaccaacc tggaagtaga cgtcattact taagccgcgc
     3241 agatcggtgg ccagtaatgc gccaacgacg cctaacggaa caaccaacat caccgagaac
     3301 ggaattgacc agctctcata gagtgcggcg agggcgagga acaccacgac cagtgaaatg
     3361 gcatacagtg caggagcctg gtttgaggat aacgcttcct gatacgacag tccggtccat
     3421 gagtagccga cgcctgccgg aagtttagcg accaggtctg ccataaattt catagcgtca
     3481 ccggtacttt tcccggccgc cgcttcacct aaaatctcca ttgacgggat gccgttgtag
     3541 cgttccaatc gcggtgaacc ataggtccat tcggtagacg agtaggcaga aagcggtgcc
     3601 atcgtgccag aggcgttgcg tacataccat tggttgatgt tatccggcaa catacggaac
     3661 ggcgtgcctg cctggacata cacttttttc acccgcccct ggttgaggaa gtcgttcacg
     3721 tagctgctgc cgaaggcggt ggaaattgtc tggttgatat cagacagcgc cacacccatt
     3781 gcctcagctt tcgcggcgtt gacgttcact ttgaacatcg gcgtatcttc caggccgttc
     3841 gggcgtaccc cggtgacctg gtcaggcgat tgcgctgcca gtgataacag ctcgtttcgt
     3901 gcctgggtta gtttttcgtg ccccaggtta ccgttgtcca gcagttccat atcaaaacct
     3961 gacgcggtac ccagttcagc caccgcgggt aagttgaacg ggaagacgac ggctttattg
     4021 atactgctta acgcaatcat tgcccgctga atgatcgcgg taaccgagtt ttcctcaccg
     4081 acacgttcag accacggctt gagactgata aacgccaggc cgttgttttg cccctggccg
     4141 ctgaagccaa agccgccaac ggtaaacacc gactgaacat tatctttctc ttttgtaaga
     4201 taataatccg tcacctgttg cagcactttc gtggtgttaa ccatggtggc accggaaggt
     4261 aactgcgcgg tggtcataaa taccccctga tcctcttctg gtaagaaaga ggtcggtgta
     4321 cgcaggaaca gcaccgccat cccggcgcaa atcagcaggt agaccaccat gtagcgaccg
     4381 gtacaacgca acagcgagcg ggtgctatcg gtatagtgct gagttgattt ttcaaacagc
     4441 gtgttgaagc gtgcgaacag ggcgttaggt ttgtgaccgc cttccggcgc ggctttcaga
     4501 atggtggcgc acagggcagg ggtcaggctc attgccacaa atactgaaag cagcatggag
     4561 gagatcagcg tgatggagaa ctggcggtag atctccccgg ttgcgccgct cataaaggcc
     4621 atcggcataa acactgcgga aagaacaaca gcaataccga ccagcgcacg ttggatctgc
     4681 cccatcgatt tatgcgtcgc ttccttcggt ggtagcttat cttccgcaat gacacgctcg
     4741 acgttctcca ccaccacgat ggcgtcatcc accagtaatc ctatcgccag caccatcccg
     4801 aacatagtta acgtgttgat ggtgaaaccg accgccgaca agatcgcaaa cgtcccgaga
     4861 ataaccaccg gtacggcaat cgtcgggatg attgtggcac ggaaattctg caaaaacaga
     4921 tacatgacca ggaagacgag gatgatagcc tcaaccagtg ttttaaaaac ttcctgaata
     4981 gaaatttcga taaacggcgt ggtgtcgtaa ggataaaccg tcttcagact tgcggggaaa
     5041 taggctgata agcggttcag ctcctctttg actgcccgcg aggtatccag ggcgtttgct
     5101 ccggtagcca gtttgatggc gatcccggca gcaggtttgc cgttatagcg cgccacggtg
     5161 gaataatctt ccgctccaag ttctacgcgg gcgacatcac gcagcagcac ttgcgaacca
     5221 tcttgctgaa ctttcaacag gattttgcca aattcttccg gcgtttgcag acgcgtctgc
     5281 acaatgatcg aggcgtttag ttgctggtct gccgcctgtg gcatgccacc cagttgacca
     5341 ccggaaatct ggttgttttg caccttaatc tgggaaataa catcggaagg taccagattg
     5401 tatttattga gtttttgcgg gtccagccag atacgcatgg cgtactcgga accaaagagc
     5461 tgtacgctac caacgcccgc ggtacggctt agcgggtctt tgatattaga cgctacatag
     5521 tccgcgatat cgtactggtt gaggctgccg ttatcagaaa taaacgccgc taccatcaga
     5581 atattactgc tcgacttatc aacgctaatc ccctgctgct gcaccgcttc aggtaatgaa
     5641 ggcatagcga gttgcagttt attttgcacc tgaacctgtg cgatatcagg agatgtccca
     5701 gtctcgaagg tcagagtgat agaggcatta cccgccgcat cactggttga agacatgtac
     5761 atcaggccat caagcccatt catattttgc tcaatcacct gagtgaccga gtcttctacc
     5821 gtttgcgcat cggcacctgg ataggtagcg ctgacggtaa tggtcggtgg cgcaatctgc
     5881 ggatactgcg caaccggtaa gttcatgatc gccagaccac ctgcaagcat cataataatg
     5941 gcaagtaccc aggcaaaaac cgggcgatca ataaaatagt tagccatgaa agtcccctta
     6001 agcctgcaac gttattgttt cgattcggtg ctggcgtttt cctggctgga ggaaattgct
     6061 cgtgctttga tacccggacg aatgcgttgc aaaccggaaa cgatcacccg atcgccagcc
     6121 tgcaagccag aggtgacaac ccactggtcg ccgatggctt tgctggcttc aatttcgcgt
     6181 agatgcacaa catcgtcttt atccagaata agcgccgttg ctttaccctg ggcgttgtgg
     6241 gtgacgcctt cctgcggcac cagtaataca ttctggcggc taccttcatc cactaatgcc
     6301 gtgacgtaca tgccaggcag caagtcacca tttgggttgg ggaaaatcgc ccgtaacgtc
     6361 acggagcccg tggtttcatc caccgtcggg tcggagaatt tcagcgtgcc ggtctggctg
     6421 tagcgtttac cattttccag attgagctgt actggcgtac tgccctgaac ctgtttgatt
     6481 tgcccactgg cgacctcttc tttcatgcgt aagaaatctt gcaccgactg cgtgagatcg
     6541 acataaatcg ggtccagacg ttgtacggta accagcgaat ctgcctgatt agcggtaacg
     6601 agtgcgccga cggtcaccga cgatttcccg ctgacgcccg taatcggcga ggtgacattc
     6661 gcgtattgca gattgatcgt cgcctgttca acagccgctt tggcgacggt gacattggct
     6721 tctgcttcat tcaactgggt gcgcgcggtg tcgtaatcct gacggctaac gtagttggtc
     6781 ttcagcaacg atgcctggcg gttaaaggtg atgcgggcat tgctggcggt agagagcgct
     6841 ttcgccagcg agcctttggc ggagtttagc tcggcctgta aaggtgcagg atcaatctga
     6901 tacagcgaat cgccctggtt cactttatcg ccttcgataa agttgcgttt aatgataata
     6961 ccgcccacct ggggacgtat ctcggcaact tcataaggaa cggttctacc gggcaattcg
     7021 ctcaacacat tgaccgaacc gggggagagt gtgacgacac cgacctcagg cgtcatggcg
     7081 gcggcgtttt ccgccgattt gtcatcgcag gcggtgagca tcgcgccgca gaataacaac
     7141 ggtattaaca gctttcttct tctgttcatt ttagtccctg aaaattcttg agaaataacg
     7201 gttctttaaa ttgcgcgcga ggagagatat gagggattta cagaccagcg gcatagtggg
     7261 gttgtcagcc agtaaggtgg gatacaggca cagtgatcga catggtgagg tcaacgacaa
     7321 aaaaaatgtt gccgttctgc caacagttcc tgtcagcatt cgggcaacga agggttctac
     7381 tggtggatac acataccagg ggaataaagt cgttttagcc tctcctttag taatcaccgg
     7441 gggtaatgaa atcagcatct gcatccctcg tcatgccagc catcaatttc agttgcttat
     7501 ctcctgacta aaaataagat gtgataccca gggtaacgat gtcgctcata cgtcttagtt
     7561 tcagcttgta cataatgttg aatttctgaa tctttaatgt ttgttgcgaa atattgagcg
     7621 tcgacgtgat attgctttca tttttatttt tcagaatcag ttgcataatt ttgaactgat
     7681 tatgtgaaaa acataattgt gaattcttat ggggcaagtg tttaccataa gtaatctcaa
     7741 gaagattttt gatactttct ttgcggctaa caaaataaat aggcgcttta gcttttagtt
     7801 tactgatgtg ataaggtgcc aaaacaataa ttctatcggc atctaatttc tccagaaatt
     7861 taatcgcttc ttcatcaagg atatgattgt gataggtatc aatgataacc ttgaagggtt
     7921 tattgcctac ttttttgatc tctgacaggg aattgatttt tatcatttcg tgattatctt
     7981 tcaactgcca aaagccctgt aaaagaaaag aatctttcgt catgagaaaa atcataactt
     8041 gctccttagc cgttatcgtt tcgaacatgt catccttttc attatttaca tccttgtccg
     8101 aatcgttgtt caatatagta aacgcctata agaactttac ccttcgtgaa ctaatacgtc
     8161 tattaacact aatcaatact tcagatttat ccatcacgca ctccatatat tgatcctaag
     8221 taagattcct ggttgttatc agcttgtaaa tagcaaacaa aaagttaacg tttgcggatt
     8281 actgcccaag aataagttat caataaccag tttgtgatct ctgaagaata ttactaaagt
     8341 taaaatgtaa tccgatttaa atatcgagtc tccttgtttc gacttaagct ggcaattgga
     8401 ttgccagctt tcttttaacg cattgaaatt acctgagtaa ataacgcatt catgtcttca
     8461 ggtttttata tagcgtctgg agacacggat atttatgcaa tgtaatcaat gccagttgca
     8521 aaaaaatgaa cccggattac ttatttaacc gcccatagca tcaacgctta cggaactctt
     8581 tgttcacttt cccactgcac ttttaaaaca ttaaacctga ctacggaaaa tatcagccat
     8641 gataatgttt gaaatggcta atttgccatg gagtgaaaaa aattagatga aattcagtag
     8701 gttgaaataa ttactagcag gtaattattt caatgatagt gcgcaattga tctacaacgc
     8761 tgcgtagcgg agagagtatt aatcggatca tagtcacatc aagtgactat gatccgggtg
     8821 acaaccggga taattattgc tgcttaacga acaaactggc gaagctgaac aggctggcgg
     8881 cgctaaatat cagttcaatt cccaccagtg tggaaaccag cgttacagac accatcggcg
     8941 ttgcaccaag gaatatccag gcaatgacga tatccagcac accaataacg agctgtagcc
     9001 agctgccttt cattgaacgc tgacgatacc aactcatcag gcgaataacc cctgcaacac
     9061 agaacaaacc ggcaataaat gccgcaatgg caaaaatgcc cagctccggt gcgcggatga
     9121 agaaatagcc gatcaataaa taggcgactg cgacgaggaa accggataat accggccaga
     9181 aattatgact gcggttgctg aataacccga caataagcgc aatacccgag cagattaata
     9241 atgcacccac tactgtgctt aaaatatcgc cagagacgaa cgggaaactg atacacagca
     9301 acccgacgat aaacagcagc acggcaataa actggattgc tctgcgatgt tttttaagca
     9361 tctccagatc aaacttcaaa attgttgcct tatctatata taacatagaa ccaccctata
     9421 aaattaagaa gaaaatcccc tgctatcaat ctatgccaaa aacgcgtcta agaatgcagt
     9481 cgatttaata aaaatttcct aattgcagta tctgatgcat ctgtaactca ttgtattgaa
     9541 ataaaaatat cttattttga tattttccat caacatgaca tatacagaaa accaggttat
     9601 aacctcagtg tcgaaattga ttcgtgacgg ctctttcact ttatagttga ggatattacg
     9661 atgaaaaaag tattaggcgt tattcttggt ggtctgcttc ttctgccagt tgtgagcaat
     9721 gcagcggatg cgcaaaaagc agctgataac aaaaaaccgg tcaactcctg gacctgtgaa
     9781 gatttcctgg ctgtggacga atccttccag ccaactgcag ttggttttgc tgaagcgctg
     9841 aacaacaaag ataaaccaga agatgcggtt ttagatgttc agggtattgc aaccgtaacc
     9901 ccagctatcg ttcaggcttg tactcaggat aaacaagcca actttaaaga taaagttaaa
     9961 ggcgaatggg acaaaattaa gaaagatatg taattccggg aatgcgttac atcgtacttc
    10021 cttgcatatt gaacaggccg gaatatcttg ctttaaaagc agctattcct cctgttcata
    10081 tataatctct atattgaatg ggttacaaaa tgaatatttc atctctccgt aaagcgttta
    10141 tttttatggg cgctgtagcg gctttgtcac tggtgaacgc acaatctgcg ctggcagcca
    10201 atgaatccgc taaagatatg acctgccagg aatttattga tctgaatcca aaagcaatga
    10261 ccccggttgc atggtggatg ctgcatgaag aaacagtata taaaggtggc gataccgtta
    10321 ctttaaatga aaccgatctc actcaaattc ctaaagtgat cgaatactgc aaaaaaaacc
    10381 cgcagaaaaa tttgtatacc ttcaaaaatc aagcatctaa tgacttgccg aattaatgag
    10441 gtacaagtaa aaaggagtag caagttgagc catcttgctg ctcctttttg catttttata
    10501 tgacagcaga atttattata cgtcttatac tcgcggcaat tgcctgtggc gctattggca
    10561 tggaaaggca aatgcgcggc aaaggagcag ggttacgcac acatgtatta attggcatgg
    10621 gaagcgccct gtttatgatt gtttcgaaat atggttttgc tgacgtgctg tctttagatc
    10681 acgtcggact cgaccccagc cgtattgctg ctcaggtggt gacgggcgtc gggtttatcg
    10741 gtgcaggtaa cattctcgtt cgtaaccaaa atattgtagg tctgacgacg gcagcggata
    10801 tctgggtgac cgccgccata ggtatggtta ttggcagcgg tatgtacgaa ctgggtattt
    10861 atggttcagt gatgaccttg ttggtgctgg aagtcttcca tcaattaacc ttccgcctga
    10921 tgaacaaaaa ttacaattta cagctgacat tagtgaacgg taatacggta tcaatgctcg
    10981 actggtttaa gcagcaaaaa ataaaaactg atttagtttc gttgcaggaa aatgaagatc
    11041 atgaagtggt cgccattgat atccagcttc atgccacaac gtcgatagaa gacctgttgc
    11101 gattattaaa aggtatggca ggggtgaaag gcgtttcaat tagctaatct gtgtggcagc
    11161 gtagccagac tcaccgtaag cctgaaattc acaccagata atcaatatgc tgatggcgta
    11221 ccagctcatt gatccgctta agattgagga tcatcatgat attgtgttta tggcagtaga
    11281 acgtttttag cgatattttc agtttctcca gaatatcttc cgttgtcatt tgattcgcaa
    11341 tgcagctcag aacctgatgt tgcttgcgcg aaagtgacac agccgcttca ggcacagagc
    11401 agtaactgcc attgatgagt gcgacaaaat caatgatagt gatagtccgc ggaacaaagg
    11461 tcaccggcac tttccccagg cagcgtttaa tattaaaggg ggagaaaata atgacatgga
    11521 caggttttaa tcgttcaatt tgagtgagaa aattagaatg aaatacatta ttcattaatg
    11581 tatcaataat gacgaaggca ttctgatgca acattacgtc gatctcttct tcgttctgta
    11641 tatgaacgac attaccttta ctcagaatgt ttttcatcgc ggtgaagaac atcgtatccc
    11701 tggtaattat aagaaacata accgactcct ggtgcggaca ttaactttat aatcagtttg
    11761 ccaaaggtaa ttaacaggac agggcttata ataataatag ttagcttact gtatattaat
    11821 tttatgtaat acatgatgca gatgctgaga aagcatctgc atctttcggt ggtgttattt
    11881 gaccagctca ggtgttacct gactcaccgc attggtgtag taaggcgcac cccagcccgg
    11941 ttccggccag aatgcaccat agccgtaatc ccacaggtta taggtggtat taaccacttc
    12001 tctcaaatgc cacacctgga tgccctgcat attcacttcc aggaagttat acgggacttt
    12061 attgataaag cctggttgtt caccctgaat ggtaccgagg atggtaacaa agtgattacg
    12121 atagttcact ggatcaagga agccgctttg tctggcgagc agtcggccct gatagttggc
    12181 ttcaatatca ggcttcgcat agctatccaa cggtaataca gcgatttcta acaacgtatc
    12241 cgttttgcca ttgataacgt tgataacctt cccaccaaag cgcgcttgtt gaccaacata
    12301 taaccccggc tggttatgaa cagcaacaaa acttttttga atatcaggtt ggttattgcc
    12361 tttgatattt tgcggaattg aactacatgc ggcaagcaaa aatgaaaggc tgaggatgag
    12421 tgcacctttt gtcatgttca tgttactatc cttatcaaca aatattaaaa ccataataga
    12481 tatttcttta tagttttcat ctgattctga ggtgatatgc ctgatagtaa cgtaaacctg
    12541 tctgatgttt atatcctttc tgtttaggtg aactcagtta attttggggc gtgacatgaa
    12601 tatccatcac cccataatat agtgcattgt tttaaaagta cataaaatct atttttattc
    12661 aat
//
LOCUS       DAJOSY010000120        12658 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJOSY010000120 DAJOSY010000000
VERSION     DAJOSY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.120"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            417..1805
                     /gene="narU"
                     /locus_tag="OHN27_003670"
     CDS             417..1805
                     /gene="narU"
                     /locus_tag="OHN27_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCQ2288045.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1887..5627
                     /gene="narZ"
                     /locus_tag="OHN27_003671"
     CDS             1887..5627
                     /gene="narZ"
                     /locus_tag="OHN27_003671"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCQ2288046.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNDFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPAGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEDRDQVQEAKK"
     gene            5624..7168
                     /gene="narH"
                     /locus_tag="OHN27_003672"
     CDS             5624..7168
                     /gene="narH"
                     /locus_tag="OHN27_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCQ2288047.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7168..7863
                     /gene="narW"
                     /locus_tag="OHN27_003673"
     CDS             7168..7863
                     /gene="narW"
                     /locus_tag="OHN27_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCQ2288048.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7860..8540
                     /gene="narI"
                     /locus_tag="OHN27_003674"
     CDS             7860..8540
                     /gene="narI"
                     /locus_tag="OHN27_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCQ2288049.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8619..9512
                     /locus_tag="OHN27_003675"
     CDS             8619..9512
                     /locus_tag="OHN27_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCQ2288050.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9608..10453)
                     /gene="nhoA"
                     /locus_tag="OHN27_003676"
     CDS             complement(9608..10453)
                     /gene="nhoA"
                     /locus_tag="OHN27_003676"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCQ2288051.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVPDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTPYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10626..11195
                     /locus_tag="OHN27_003677"
     CDS             10626..11195
                     /locus_tag="OHN27_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCQ2288052.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMASAWSMP
                     VEFELPRVAIVVDKSTWTRELIEHNGKFGIVIPGVAATNWTWAVGSVSGRDEDKFNCY
                     GIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11192..11425)
                     /gene="pptA"
                     /locus_tag="OHN27_003678"
     CDS             complement(11192..11425)
                     /gene="pptA"
                     /locus_tag="OHN27_003678"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCQ2288053.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSMNA"
     gene            complement(11525..12643)
                     /locus_tag="OHN27_003679"
                     /pseudo
     CDS             complement(11525..12643)
                     /locus_tag="OHN27_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041204891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3002 a         3246 c         3246 g         3164 t
ORIGIN      
        1 actgtaataa cgattattac aatcagacaa taagagagga accgatagac cttctggaca
       61 gatcgttttc ctccagctca tggccttttg agtgaataaa ttattgccaa ttctataagt
      121 gtcttgtctt gttaagaata tagttaatat acaacccttt ggattcatca tctgaagggt
      181 tgaatcatga catgcgtaca tagcttaaca cccgtttaat ggttagcatg ttagttacat
      241 ttagtaacac atatctggct acttaatccc cattctgccc ccgcggttac gctttcttat
      301 ctattctttc aatcagcact tcacccaatt catccttaat tctaataact ggctattgcg
      361 ccttacctcg tgttttgtct ttttttcatt accaatgtgt gcatgtgagg aacaatatgg
      421 cactgcaaaa tgagaaaaat agtcgttatc ttttgcgcga ctggaaacca gaaaatccgg
      481 ccttctggga aaataaagga aaacatattg ctcgaagaaa tctctggata tcagtcagtt
      541 gtctacttct tgccttctgt gtctggatgc tatttagcgc agttaccgtt aatctcaata
      601 aaatcggttt taattttact accgatcaac tctttttatt aaccgcatta ccctccgttt
      661 ctggcgcatt attgcgtgtt ccctactcct ttatggtgcc tatattcggt ggacgccgat
      721 ggacggtttt tagtactgca atcctgatta ttccttgcgt ctggctcgga attgccgtgc
      781 aaaatccgaa tactcctttt gggatattta tcgttatcgc tttgctatgc ggttttgcag
      841 gtgcaaactt tgcttcgagc atgggcaata tcagtttctt ctttccaaaa gccaaacaag
      901 ggagcgctct tgggattaat ggcggattag gaaacttagg tgtaagtgta atgcagctgg
      961 ttgcaccgct ggtcattttt gtacctgtat ttgcctttct cggcgtcaat ggcgtaccgc
     1021 aggccgacgg ttcggtgatg tcgctggcga atgccgcatg gatttgggta ccgctactgg
     1081 cgattgccac gatcgccgca tggtcaggga tgaatgatat cgccagttca cgcgcctcaa
     1141 ttgccgacca gctccctgtc ttacaacgcc tgcatctctg gctgctgagc ctgctttacc
     1201 ttgccacctt cggttcgttt atcggttttt ctgcgggttt tgccatgctg gcaaaaaccc
     1261 agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg cccatttatc ggtgccatcg
     1321 cgcggtcggt tggtggtgct atttccgata agttcggcgg cgtgcgggtg acgttgatca
     1381 actttatttt tatggcgatt ttcagtgccc tgctgttcct taccttaccg ggcacaggct
     1441 ccggtaattt catcgccttt tacgccgtat ttatggggct gtttctgact gcgggtctgg
     1501 gaagtggttc tactttccag atgatcgccg tcatctttcg ccagataacc atttatcggg
     1561 taaagatgaa aggcggtagt gatgagcaag ctcagaaaga agccgtcacc gaaacggcgg
     1621 cggctctggg ctttatctca gccattggcg cagtgggcgg cttttttatt ccgcaggcgt
     1681 ttggcatgtc gctcaatatg accggctctc cggtcggcgc gatgaaagtg tttttaatct
     1741 tctacatcgt ttgtgtgctg ctgacctggc tggtttatgg tcggcggaag ttcagccaaa
     1801 aataagagat cgatgcctga tgtacaaccg tgtcgcatcc gacaaggcat cagatcccaa
     1861 ttcattcctg gagcaggagt catgtcatga gtaaactttt ggatcgcttt cgctacttca
     1921 aacaaaaggg cgaaaccttt gccgatggtc acggacaggt gatgcatagc aaccgcgact
     1981 gggaggacag ctatcgccag cgttggcagt tcgacaaaat cgtgcgttcc acccacggtg
     2041 ttaactgtac aggctcctgt agctggaaaa tctacgttaa aaatggtctg gtgacctggg
     2101 aaatccaaca gaccgactac ccgcgcactc gccctgacct gcccaatcat gaacctcgcg
     2161 gctgcccgcg tggcgcaagt tactcctggt atctttacag cgctaaccgc ctgaaatacc
     2221 cgctcattcg taaacgactg attgaactgt ggcgcgaagc cctcaagcaa cacagcgatc
     2281 cggtactggc gtgggcatcg attatgaacg atccgcaaaa gtgcctgagc tacaaacaag
     2341 tgcgtgggcg cggcgggttt atccgctcca actggcagga actaaaccag ctgattgccg
     2401 ccgctaacgt ctggaccatc aaaacctacg gcccggatcg cgttgccggt ttctcgccga
     2461 tcccggcgat gtcgatggtt tcttacgccg ccggaacgcg ttatctgtcg ctgcttggcg
     2521 gcacctgttt aagtttctac gactggtatt gcgacctgcc gcccgcctcg ccgatgacct
     2581 ggggcgagca aaccgacgta ccggaatctg ccgactggta taactccagc tacatcatcg
     2641 cctgggggtc taacgtaccg cagacacgta cgccggacgc ccacttcttt accgaagtac
     2701 gctacaaagg cactaaaacc atcgccatta cccctgacta ctctgaagtg gccaaattgt
     2761 gcgaccagtg gctggcaccg aaacaaggca ctgatagcgc cctggcgatg gcaatgggcc
     2821 atgtgatttt aaaagagttt catctcgata atcccagcga ctactttatc aactactgcc
     2881 gccgctacag cgacatgccg atgctggtaa tgctggagcc tcgcgacgat ggtagctacg
     2941 ttcccgggcg catgatccgc gcatctgacc tggtggatgg actgggcgaa agcaacaatc
     3001 cgcagtggaa aaccgtagca gttaataccg caggtgaatt ggtagtgccg aacggttcga
     3061 ttggtttccg ctggggagaa aaaggcaaat ggaatctgga atccattgcc gccggtacgg
     3121 aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc tgttgcaggc gtggccttcc
     3181 cctactttgg cggcattgaa aatccgcatt ttcgcagcgt aaaacacaat ccggtgctgg
     3241 tgcgccaatt gcccgttaaa aacctgacgt tagtcgatgg caacacctgt ccggtggtca
     3301 gcgtttatga tttggtactg gcgaattacg gcctcgatcg cgggctggaa gatgaaaaca
     3361 gtgcgaaaga ttacgctgaa atcaaaccgt acaccccagc ctggggtgag caaattaccg
     3421 gcgtgccgcg ccagtatatt gaaaccatcg ctcgtgaatt tgccgatact gcccataaaa
     3481 cgcatgggcg ctcaatgatt atcctcggtg ctggcgtcaa ccactggtat cacatggata
     3541 tgaactaccg tgggatgatc aatatgctga tcttctgcgg ttgtgtcggg caaagcggcg
     3601 gcggctgggc gcattatgtc ggtcaggaaa aactgcgccc acaaaccggc tggttgccgc
     3661 tggcctttgc gctcgactgg aaccgcccac cgcgccagat gaacagtacc tcgtttttct
     3721 acaatcattc cagccaatgg cgctatgaaa aagtctctgc gcaggagtta ctttcaccgc
     3781 tcgccgatgc cagtaagtac agcggtcatc tgattgattt caacgttcgc gccgaacgta
     3841 tgggctggct gccctctgcg ccgcagctgg ggcgtaaccc gctcgggatt aaagctgaag
     3901 ccgacaaagc aggattatcc cccacagaat ttaccgccca ggcgctgaaa tcgggcgatt
     3961 tacgtatggc ctgcgaacaa ccagatagcg gcagcaatca tccgcgtaat ttgtttgtct
     4021 ggcgttctaa cctgcttggc tcctccggca aaggccacga gtatatgcag aagtatctgc
     4081 tggggaccga aagcgggatt cagggcgagg aactcggtgc cagcgacggg atcaaaccgg
     4141 aagaagtcga gtggcaaact gcggcgattg aaggcaagct cgacctgctg gtgacgctcg
     4201 acttccgcat gtccagcacc tgcctgttct ccgatattgt tctgcctacc gccacctggt
     4261 acgaaaaaga cgatatgaat acctcggata tgcatccgtt tattcatcca ctttctgcgg
     4321 cagtcgatcc ggcctgggag tcacgcagcg actgggaaat ctacaaaggt attgccaaag
     4381 cattttcgca agtgtgcgtg ggtcatcttg gcaaagaaac cgacgtggta ttacaaccac
     4441 tgctgcacga ctctccggca gagctctcac agccgtgtga agtcctcgac tggcgcaaag
     4501 gcgaatgcga tctgattcca ggtaaaaccg cacccaatat tgtggcggtg gagcgcgact
     4561 accctgctac ctatgaacgc tttacctcgc tcgggccatt gatggacaaa cttggcaacg
     4621 gcggtaaagg gatttcgtgg aatacgcagg atgaaatcga tttcctcggt aaactcaatt
     4681 acaccaagcg tgatggccca gcgcaggggc gtccgctgat tgacaccgcc attgacgctt
     4741 cagaagtgat tctggcactg gcaccagaaa caaacggtca tgttgcagtt aaagcgtggc
     4801 aggcgctggg cgagatcacc ggacgcgaac atacccatct ggcgctgcac aaagaggacg
     4861 agaagattcg ctttcgcgat attcaggcgc agccacgtaa aattatctcc agccccacat
     4921 ggtctggtct ggaaagcgat cacgtctcct ataacgcggg atacaccaac gttcatgagt
     4981 taattccgtg gcgcacgctg tcgggacgcc agcagctcta tcaggatcat ccgtggatgc
     5041 gtgcttttgg tgaaagcctg gtggcttatc gcccgcctat cgacacccgt agcgtcagtg
     5101 agatgcgcca gataccgcca aacgacttcc cggaaaaagc acttaacttc ctgacgccgc
     5161 accagaaatg gggcattcac tcaacctaca gtgaaaacct gctaatgctg acgctctctc
     5221 gcggtggacc gattgtctgg atcagcgaaa ccgatgcccg agaactgacc attgtcgata
     5281 acgactgggt ggaagtgttt aacgccaatg gcgcgctgac ggcccgcgcg gtggtcagcc
     5341 aacgtgtacc ggcgggtatg accatgatgt atcacgctca ggaacgcatt atgaatattc
     5401 ctggttcgga agtaactggc atgcgcggcg gtattcataa ctcggttacc cgcgtttgcc
     5461 cgaaaccaac gcatatgatt ggcggttacg cgcagctggc ctggggcttt aactactacg
     5521 gcaccgtcgg atcgaaccgc gatgagttca tcatgatccg caagatgaag aacgttaact
     5581 ggctggatga tgaagatcgc gatcaggtac aggaggcgaa aaaatgaaaa tacgttcgca
     5641 agtcggcatg gtgcttaacc tcgacaaatg tatcggctgc catacctgtt cggtgacctg
     5701 taaaaacgtc tggaccggac gcgaaggcat ggagtacgca tggtttaaca acgtcgaaac
     5761 caaaccgggc attggttatc cgaaaaactg ggaagatcag gaagagtggc aaggcggctg
     5821 ggtccgcgat gtgaatggca agatacgccc gcgtctgggt agcaagatgg gcgtaataac
     5881 caaaatcttc gccaacccgg tggtgccgca gattgatgat tactacgaac ctttcacctt
     5941 cgactacgaa catttgcata gcgcaccgga aggcaaacat atccctactg ctcgcccgcg
     6001 ttcgctgatt gacggtaagc ggatggacaa agtgatctgg gggccaaact gggaagaact
     6061 gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac ttcgaggcca tgcaaaagga
     6121 gatgtacggg cagtttgaaa acaccttcat gatgtacctg ccgcgcctgt gcgaacactg
     6181 cctcaatccc agttgcgtgg cgacctgccc aagcggcgct atctacaaac gcgaagaaga
     6241 cggcattgtg ctgattgatc aggataaatg ccgtggctgg cgtttgtgca ttagcggttg
     6301 tccgtacaaa aaaatctact tcaactggaa aagcggcaag tcagaaaaat gtatcttctg
     6361 ttatccgcga attgagtcag gccaacccac cgtgtgctca gaaacctgcg tgggtcgcat
     6421 ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatt gaggaagcgg caagcaccga
     6481 gcgcgaagtt gacctctatg agcgccagtg cgaagtgttc ctcgatccac acgatccctc
     6541 agtgatcgag gaagccctga aacaaggtat tccacaaaac gtgattgaag ctgcccagcg
     6601 ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg ctaccgctgc accctgaata
     6661 tcgcaccctg ccaatggtct ggtacgttcc tccgctgtcg ccgattcagt cctacgcaga
     6721 tgcgggcggt ttgccgaaaa gcgaaggcgt gctgcccgcc atcgaaagcc tgcgtattcc
     6781 ggtgcaatat ctcgccaata tgttgagtgc cggcgatacc ggtccggtac tgcgggcgct
     6841 gaaacggatg atggcgatgc gccactatat gcgttcacaa accgtggaag gcgttactga
     6901 tactcgtgcc atcgacgaag taggcctgag cgtcgcccag gtcgaagaga tgtatcgtta
     6961 cctcgccatt gccaactatg aagatcgttt tgtcatcccg acgagccatc gggaaatggc
     7021 gggcgatgcc ttcgcagaac gcaacggctg cggttttacc tttggcgacg gttgtcacgg
     7081 ctcggacagc aaattcaacc tgttcaacag tagccgtatc gatgccatca acatcaccga
     7141 agtgcgcgac aaagcggagg gcgaataatg cagatcctca aagtgatcgg cctgttgatg
     7201 gagtatccgg acgagctgtt gtgggaatgc aaggaggacg cgctggcgtt gatccgccgc
     7261 gacgcgccga tgcttacgga tttcacccac aacctgctta acgcgccgct gctggataaa
     7321 caggccgaat ggtgcgaagt gtttgaccgt ggacgtacca catcgctgct gctgttcgaa
     7381 catgttcatg ccgagtcccg cgatcgcggc caggcaatgg tggacctgct ggcggagtat
     7441 gaaaaggtcg gcctgcaact ggattgtcgg gaactgcccg attatctacc gctgtatctg
     7501 gagtatttaa gcgtactgcc tgacgatcag gcgaaagaag gattgctcaa tgttgcgccg
     7561 atcctcgccc tgcttggcgg tcgcttaaaa caacgcgagg caccgtggta cgcgttgttt
     7621 gatgctctgc tgcaactggc gggaagctct ctttcaagtg acagtgtcac aaaacaagta
     7681 aacagcgaag agcgcgatga cacccgccag gcgcttgatg cagtgtggga agaggaacag
     7741 gtgaagttta ttgaagataa cgccacggcg tgtgacagct cgccgcttaa tcaatatcag
     7801 cgacgcttta gccaggatgt cgcgccgcaa tatgtcgaca tcagtgcggg aggtgggaaa
     7861 tgattcagta tctgaacgtc tttttttacg atatctaccc gtacatttgt gcgacggtgt
     7921 ttttcctcgg cagctggctg cgctacgact acgggcagta cacctggcgc gcctcctcaa
     7981 gtcaaatgct cgataaacgc gggatggtga tatggtcgaa tctgttccat atcggcattt
     8041 tggggatttt cttcgggcac ttgttcggca tgttaacgcc gcactggatg tacgcgtggt
     8101 ttttgccagt ggcagcgaaa caactcatgg cgatggtgct cggcggtatt tgcggtgtgt
     8161 tgacgttgat tggcggcgca ggactgctgt ggcgcaggct gacaaatcag cgcgtgcgtg
     8221 ccacttccac tacgccggat atcatcatca tgagcatttt gctgatccag tgtctgttgg
     8281 ggctaagtac cataccgttt tcagcacagt atcctgatgg tagcgaaatg atgaagctgg
     8341 tgggttgggc gcaaagcatt gtcactttcc gtggtggatc gtcagagatg ctcaacggcg
     8401 tagcgttcgt cttccgcctg catctggtgt tgggaatgac catcttcctg ctcttcccgt
     8461 tcacccgact ggtgcatgtg tggagcgcgc cgtttgagta ctttactcgt cgatatcaaa
     8521 ttgtgcgttc gcggcgataa tttcaccctg gccggatagc ccttcgctct ccggccactt
     8581 gcaggctatg atagttctcc agtcacagga gaacgttaat gaaaccgcag gtgtaccacg
     8641 tcgatgcttt tacctcacaa ccgtttcgcg gcaattctgc cggtgttgtt ttccccgccg
     8701 ataatcttag cgaagcgcaa atgcagctta tcgcccgcga gttaggccat tcggaaactg
     8761 cttttctgct gcacagcgac gacagcgatg tgcgcatccg ctactttacg ccaacggttg
     8821 aagtgccgat ttgcggtcac gcgacagtag ctgcgcacta tgtacgtgcg aaggtgttag
     8881 gtttaggaaa ttgcacgatc tggcaaacat cgctggcagg aaaacatcgc gtgactatcg
     8941 aaaagcacaa cgatgattat cgtatttcgc tggaacaagg tacgccgggc tttgaactgc
     9001 cgctggaggg tgaagtacgc gcggcgatta tcaacgcatt gcatcttact gaagacgata
     9061 ttctgccggg tttgccgatc caggtcgcga cgaccgggca ttctaaagtg atgatcccat
     9121 tgaagccgga agtggatatc gacgctcttt cgcctgacct taatgcgctg accgctatca
     9181 gtaaacagat tggctgcaat ggtttcttcc cgttccagat ccgtccaggc aaaaacgaaa
     9241 ccgatggtcg catgttctcg cctgcgattg gtattgtgga agatccggtg actggcaatg
     9301 ccaatggccc gatgggtgca tggttggtac atcacaacgt attgccccac gatggcaagg
     9361 tgctgcgtgt taaaggccat caggggcgcg cattggggcg tgacggcgtg attgaagtga
     9421 cggtgacaat tcgtgataac caaccggaaa aagtcaccat ttctggcgcg gcggtgattt
     9481 tgtttcatgc cgaatgggca attaaactct gaaatcgttg cctgatgcgc ttcgcttatc
     9541 aggtctacaa ccgagctaat gtatgccgga taaggcgttt acgccgcatc cggcaacctg
     9601 acataaatta ttttcccgcc tccgggtgcg tatcaaacgc cgccatcacc agcgctaact
     9661 catccacggt aaagccatgt ttcgcatcat ccacgcccag accaaactgt tcttgcatca
     9721 cagcatataa tgacgccaca tccggtagat ttcgctgctc caccgcgtgc ccattttcat
     9781 aaggggtaaa atgaaaattg gtcagtgtca gcttgccgcc gtccggcaaa tggcggcaca
     9841 tcagcaaatg atggcgaaaa tgcgactgcg gccagtgcgc cgaccagaaa ttgcccatca
     9901 cataatcgct ttgttgctgc tcgcagagat caaaacggta catcgactgc cagtgctgat
     9961 gatgattaaa ctgcaacacc caatcatcac cctcctgcaa caaccgatac tctccgtgtg
    10021 gcgtggtctg cacgagatcg ggaactaaac gaatcggcgc ggttagcgtc tgcccaccga
    10081 aaccgacatc agcaatccat ttttcctctt ccagttccac caacagcaaa cgatgggtgc
    10141 gcggcggtaa tgctggcgga tttgataaca ctacgcgccc taacaagctg cgaacgttaa
    10201 accccagctc gcgtaacacc cgctcaaaca cgccattctg ctcaaaacag taaccgccac
    10261 gacgggctat caccagtttc tcttccagcg attgatcatc aagctgtatt tccctcggca
    10321 gcaaaacgtc aaggttttca aacggaatgg tgcaattgtg tttcaggtgc aatgcacgaa
    10381 gcgtatcaat attgaccgca gcagctcccg accagttaat acgggcaaaa tagtgattca
    10441 gaatgggcgt catcaggcgt ttcccttagt ggttttctca acttataacc tggaatagac
    10501 gccgcaccgg ctattttgtg cgatcgcgga catgcatcac gttcgcaaat cgccaatccc
    10561 gttaatttcg ttacttattg cgttacggac tacgcttaaa aaaaccttct tcgcgaggcg
    10621 ataccatgag ccgattcatc cccatagaat tacaccatgc cagccgtctg ttaaatcatg
    10681 gcccaacggt cttgatcacc agtttcgatg agcaatccca gcggcgtaac attatggctt
    10741 cagcctggtc aatgccggtg gagtttgaac ttccacgcgt ggcgattgtg gtagataaat
    10801 cgacatggac cagggagttg attgaacata acggtaaatt tggcatcgtt atcccgggcg
    10861 ttgcagcaac taactggacg tgggcggtgg gaagtgtgtc ggggcgtgat gaagataaat
    10921 ttaattgcta tggcattccg gttgtgagag gcccggtatt tggtttgcct ctggtcgagg
    10981 aaaaatgtct ggcgtggatg gagtgtcgat tgctacctgc gacttctgcg caagaagaat
    11041 acgacacgct gtttggcgaa gtagtatcag cagcggcaga cgcacgggta tttgtcgaag
    11101 gccgctggca gtttgatgat gataagctca atacgttgca tcatttaggt gctgggacgt
    11161 ttgttaccag cggcaagcgt gttacggcgg gttaagcatt catgctgtaa ccaggtttct
    11221 ttatcaaagc ctccatttgg ggcgcgattt cggcatccca gatagcttgc caagattctg
    11281 gttgaatctg ctgtagagca atgcttatcg aactgtcttt actgttcaga tgacgaataa
    11341 taacgtcggt aatatctgca gcaagtgctg ctttttgttg ttcgtccagt tcacgcggaa
    11401 aacatttaat gtcgatgtgc ggcatgatta cgctcctttt gaaagatgtc agtaaaatta
    11461 acacacattg tgaatgtcat gtgtccggta aagcgtttat acgacaataa tgcccagggc
    11521 aagattacga aagcccgctc cccgcaagga ctgacgccag atagtttctg tccatggctg
    11581 cttttcgcat cttacgtctt aaccctgcct tgaatacctt atcattcgtc aaaacattaa
    11641 tagcgatgtg ccgtatccct gaaaataatt ctgctgcatt tcctcttctt attttgcagt
    11701 cgtcttcatt cattaccacg tccagacgcc agtgcagctt attctccacg tgccagtggt
    11761 ttcggattgc tgtggcgaac ttctctgcgg ttaaatcagc agaactgata taatatctga
    11821 ccgtcatttc tggctctttc ttttgttctg ctattatgga ccgaaaggag actgccacgc
    11881 ataatttctt cagccctttc cattcaaacg tgaaatcaat aagttcatca gggacatcgc
    11941 aaacaatatg aagacggatt tcttctctgc cgtgactctt ttcactcatt gcgtaactgt
    12001 catgctctgg attatttaat tctttcagcg gaaatttttc ctcaaaggct ttatttagcc
    12061 gcccctggtt tccttttacc gcgaataaat aatcacctcc ctgtttttgt atcttttctg
    12121 caatatcttt ctggcagccc atcgcatcag ttgtgatgat ttttccttta atatccatca
    12181 tgttaagaag ttcaggaatg gctgtaatct cattggattt ctcatccgtc ttgatctgtc
    12241 cgaggaccag actatgcatt gttgagaacg cactaatgac atgaatcgct cccctgcgac
    12301 gactcttgtc ataagagtgc cggagcgttt ttccatcaat tgcaatgacg tctttatcat
    12361 ctgaagaatg gcagtcacgc atccagttaa taaagcactc gtgaaatttt gcaggactga
    12421 tacaggatac aactctggca atgatatcgt gaacaggaat accattttca aaatcaccat
    12481 atcacttcaa aacatcggga tgtgtttccc caaaatcctc tatatcttcc cagccttctg
    12541 caccagaaat aacggcacaa atagtcaaca gtagaatatc cgataattta tgttccactt
    12601 tccaggcttg tctgtaatcg gggataatag aaatatgttc catcaatttt ttaagttc
//
LOCUS       DAJOSY010000121        12174 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJOSY010000121 DAJOSY010000000
VERSION     DAJOSY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.121"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            91..1317
                     /gene="mdtM"
                     /locus_tag="OHN27_003680"
     CDS             91..1317
                     /gene="mdtM"
                     /locus_tag="OHN27_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCQ2288054.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKAIKLMAIITSIVLLAPIIGPLSGAAL
                     MHFVHWKVLFAIIAAMGLVSFVGLLLAMPETVKRGAVPFSAVSVLRDFRNVFCNRLFL
                     FGAATIALSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANTIVARFIK
                     DPTEPRFIWRAVPIQLVGLALLIVGNLLSPHVWLWSVLGTSLYAFGIGLIFPSLYRFT
                     LFSNNLPKGTVSASLNIVVLTVMSVSVEIGRWLWFNGGRLPFHLLAVVAGLFVVLTLA
                     GLLKRVQLHQASELAV"
     gene            1362..2642
                     /locus_tag="OHN27_003681"
     CDS             1362..2642
                     /locus_tag="OHN27_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCQ2288055.1"
                     /translation="MNKLIELRRAKMLALSLLLTAVATFIVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDSSRRFIAQQIVRWLETEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISHDRAHQIRHAFDRATFKLIDKLKNDP
                     EMATRADAVKAYLKEDEAFNRYLGELWADLRQWLKADINSDDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDAQDMSLQIELNIGKD
                     LQFIRVNGTLVGGSIGLVLYLLSQIPSFLSLSNF"
     gene            2652..2891
                     /locus_tag="OHN27_003682"
     CDS             2652..2891
                     /locus_tag="OHN27_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HCQ2288056.1"
                     /translation="MAAITLSPAERERLRDELQNYCAENFNVELEQFDAEFFLDFICE
                     KLGPVFFNAGIEEAIRTHLAWSERISEEMDLKKIL"
     gene            2987..4138
                     /locus_tag="OHN27_003683"
     CDS             2987..4138
                     /locus_tag="OHN27_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HCQ2288057.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLAVMDFKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVQDDASRALWKAEMLRLQTAVEERFGKE
                     ITEEALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALIDE
                     LDAMTARVRQQWEEGQRLDTRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            4148..4915
                     /locus_tag="OHN27_003684"
     CDS             4148..4915
                     /locus_tag="OHN27_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288058.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            4912..5169
                     /locus_tag="OHN27_003685"
     CDS             4912..5169
                     /locus_tag="OHN27_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCQ2288059.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            5234..6094
                     /locus_tag="OHN27_003686"
     CDS             5234..6094
                     /locus_tag="OHN27_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCQ2288060.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            6162..7340
                     /locus_tag="OHN27_003687"
     CDS             6162..7340
                     /locus_tag="OHN27_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2288061.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(7353..7907)
                     /gene="kptA"
                     /locus_tag="OHN27_003688"
     CDS             complement(7353..7907)
                     /gene="kptA"
                     /locus_tag="OHN27_003688"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCQ2288062.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            8157..8840
                     /locus_tag="OHN27_003689"
     CDS             8157..8840
                     /locus_tag="OHN27_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288063.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGMSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            8837..9298
                     /locus_tag="OHN27_003690"
     CDS             8837..9298
                     /locus_tag="OHN27_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCQ2288064.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            9311..10483
                     /gene="iadA"
                     /locus_tag="OHN27_003691"
     CDS             9311..10483
                     /gene="iadA"
                     /locus_tag="OHN27_003691"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCQ2288065.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            10548..11459
                     /gene="hypT"
                     /locus_tag="OHN27_003692"
                     /gene_synonym="qseD"
     CDS             10548..11459
                     /gene="hypT"
                     /locus_tag="OHN27_003692"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCQ2288066.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(11452..11844)
                     /gene="iraD"
                     /locus_tag="OHN27_003693"
     CDS             complement(11452..11844)
                     /gene="iraD"
                     /locus_tag="OHN27_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCQ2288067.1"
                     /translation="MMRQSRQAVLPEISGNKTSSLRKSICSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(11841..11924)
                     /gene="idlP"
                     /locus_tag="OHN27_003694"
     CDS             complement(11841..11924)
                     /gene="idlP"
                     /locus_tag="OHN27_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCQ2288068.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         2706 a         3106 c         3188 g         3174 t
ORIGIN      
        1 atttgcccgt ctcgcctgcc tttcgtcatt accgacggta ttgaatttcg ttttccccgt
       61 tggggttctc cggacaagga gttgtttgtt atgccacgtt tttttgcccg ccatgccgcc
      121 acgctgtttt tcccgatggc gttgattttg tatgactttg ccgcgtacct gtcgacggat
      181 ctgatccagc cggggatcat taatgtggtc cgtgatttta atgccgatgt cagtctggcc
      241 ccggctgccg tcagtctcta tctcgctggc ggtatggcgt tacagtggct gctggggccg
      301 ctttccgaca ggattggccg caggccggtg ctgattaccg gggcgcttat ttttaccctt
      361 gcctgcgccg cgacaatgtt cacaacgtct atgacacagt ttcttatcgc gcgtgcaatt
      421 cagggtacca gtatctgttt tattgctacc gttggttatg tcacggtgca ggaagccttt
      481 gggcagacga aagccattaa actgatggcg attattacct ccattgtgtt gctggccccc
      541 ataatcggcc ctctttccgg cgcggcgtta atgcactttg tccactggaa agtgcttttt
      601 gccattattg cggcgatggg gcttgtctcg tttgtcggcc tgttgttggc gatgccagaa
      661 acggtgaagc gtggtgcggt tccgttcagc gctgttagcg tactgcgcga ttttcgtaac
      721 gttttttgca accggctgtt cctctttggc gcggcaacca tcgccctcag ctacatcccc
      781 atgatgagtt gggttgcggt gtcaccggtt atcctgatcg acgcgggcgg cttaacgact
      841 tcgcaatttg cctggacaca ggttccggtc ttcggcgcgg tgattgtcgc caacaccatc
      901 gtggcgcgat ttatcaaaga tccgaccgaa ccgcggttta tctggcgtgc cgtgcccatt
      961 caactggtcg gcctcgcgct attgattgtc ggtaatctgc tgtcgccgca cgtctggctg
     1021 tggtcggtgc ttggtaccag cctgtacgct tttggtattg gtttgatttt cccgtcgcta
     1081 taccgcttta cgctgttttc gaacaacctg ccgaagggca ccgtgtcggc atcgctgaat
     1141 atcgtggttt taacggtgat gtcggtctcg gtcgaaattg gtcgctggct gtggtttaac
     1201 ggcggtcgtt tgccgtttca tctgctggcg gtagttgcgg ggctgtttgt tgtgttaacg
     1261 ctggcggggt tactgaaacg cgtgcagttg catcaggcaa gcgaactggc ggtataggca
     1321 ccttttgcgc aatccagccg tcaggctcta tgcttaaggt tatgaataaa ctcattgaac
     1381 tcagacgcgc caaaatgctg gcgctctcat tgctgctgac agcggtcgcc acgtttatcg
     1441 ttacgctatt tttgccaccc aatttttggg taagcggcgt gaaggcgatt gccgaagcgg
     1501 cgatggtcgg tgcgctggca gactggtttg cggtggtggc gctatttcgc cgcgtgccga
     1561 tcccgattat ttctcgtcat acggcgatta tcccgcgtaa taaagaccgg attggcgaaa
     1621 atctcggtca gttcgtgcag gaaaaatttc tcgataccca atcgctggtg gcgttgattc
     1681 gacgtcatga accggcgtta ttgatcggta actggtttag tcagccggag aacgcccgcc
     1741 gcgttggtca gcatctgttg cagatcatga gcggttttct tgagctgacc gatgatgcgc
     1801 gcattcagcg cctgctcaag cgggcggtac accgggcgat cgataaggtc gatctctccg
     1861 gcaccagtgc gctaatgctg gagagcatga ccaaaaacga tcgccatcag gtgctgctcg
     1921 atacgttaat tgcccagctt attgccctgc tccagcgcga cagttcgcgg cgttttattg
     1981 cccaacagat cgtccgctgg ctggagactg aacacccgct gaaagcgaaa atcctgccta
     2041 ctgaatggct cggcgagcac agcgctgaac tggtttcgga cgcggtcaat tcgctgctgg
     2101 atgatatcag ccacgatcgc gcccatcaga tccgccatgc gtttgatcgc gccacgttta
     2161 aactgatcga caagctgaaa aacgatccgg aaatggcaac ccgcgccgat gcggtaaaag
     2221 cttatctgaa agaagatgaa gcctttaacc gctatctggg cgagttatgg gccgatcttc
     2281 gccagtggct gaaggctgat attaacagcg atgattccag ggtaaaggag cggatcgccc
     2341 gcgcgggtca gtggtttggc gaaacgctga tcgcggatga tgcgttacgc gcctcgctga
     2401 atggtcatct ggagcaggcc gcgcaccggg tcgcacctga gttttccgcc ttcctgacgc
     2461 gccacatcag cgacacggtc aaaagctggg atgcgcagga tatgtcactg caaatcgaac
     2521 tcaacattgg taaggattta cagtttattc gcgttaacgg cacgctggtt ggtggttcca
     2581 tcgggctggt gttatatctg ttgtcgcaaa ttccgtcatt cctgtccctg tccaattttt
     2641 aaggagcgaa aatggctgcg attaccctct caccggcaga gcgtgaacgt ctgcgcgatg
     2701 agttgcaaaa ctactgtgcc gaaaatttta atgtcgaact tgaacagttc gacgcggaat
     2761 tcttcctcga tttcatctgt gaaaagctcg gtcctgtgtt ttttaacgcg ggaattgaag
     2821 aagcgatccg cacccatctc gcctggagcg aacggatttc agaagagatg gatctgaaaa
     2881 aaatactatg agtacattcc gcgcgggaga tcactaaaac catacataat gcatggttat
     2941 aatacggctc tatttatcat ctcccgtgtt acggggaagg aaaatcatgt cacttatcac
     3001 cgatctaccc gccattttcg atcaattctc tgaagctcgc cagaaaggtt ttctcgccgt
     3061 tatggatttc aaagagcgcg gcattccgct ggttggcacc tactgcacgt tcatgccgca
     3121 agagatcccg atggctgccg gtgcggttgt ggtttcactc tgctccacct ccgatgaaac
     3181 catcgaagaa gccgagaaag acctgccgcg caatctctgc ccgctgatca aaagcagcta
     3241 cggctttggc aaaaccgata aatgccccta cttctacttt tccgatctgg tggttggtga
     3301 aactacctgc gacggcaaaa agaaaatgta tgaatacatg gcggagttta agcccgttca
     3361 tgtgatgcaa ttgcctaaca gcgtgcagga tgacgcctca cgcgcgttgt ggaaagccga
     3421 gatgctgcgc ctgcaaacag cggtagaaga gcgttttggc aaagagataa ccgaagaggc
     3481 gctgcgcgat gccattgcac tgaaaaaccg cgaacgccgt gcgctggcca atttttatca
     3541 tcttgggcag ttaaatcctc cggcgttaag cggcagcgac attctgaaag tggtttacgg
     3601 cgcaacattc cgttttgaca aagaagcgct gatcgacgaa ctcgatgcaa tgaccgcccg
     3661 cgttcgccag cagtgggaag agggtcagcg gctggacacg cgtccacgca ttctgataac
     3721 tggctgcccg attggcggtg cggcagagaa agtggtgcgt gccatcgaag agaatggtgg
     3781 ctgggttgtc ggttatgaaa actgcaccgg ggcgaaagcg acagagcaat gcgtggcgga
     3841 aacgggcgat gtgtacgacg cactggctga taagtatctg gcaatcggct gctcctgtgt
     3901 ttcgccgaac gatcagcgcc tgaaaatgct cagccagatg gtggaagaat atcaggtcga
     3961 tggcgtagtt gatgtgattt tgcaggcgtg ccatacctac gcggtggaat cgctggcgat
     4021 taaacgtcat gtgcgtcagc agcacaacat tccttatatc gctattgaaa cagactactc
     4081 cacctcagat gtcgggcagc tcagtacccg tgtcgcggcc tttattgaga tgctgtaagg
     4141 agtggcagtg acatattcga ttggcattga ttctggttct accgccacca aagggatctt
     4201 actggcagac ggcgtgatta cgcgccgttt cctcgttcca acaccctttc gtccggcaac
     4261 agcaattact gaagcctggg aaactctgcg cgaagggtta gagacaacgc cgtttctgac
     4321 gctcaccggc tatggacggc aactggtgga ttttgccgat aaacaagtga cggaaatctc
     4381 ctgtcatggg ctgggcgcac ggtttcttgc gccagcaacg cgcgcggtaa tcgacatcgg
     4441 tggtcaggac agcaaagtga ttcagcttga tgatgacggt aacctgtgcg atttcctgat
     4501 gaatgacaaa tgcgcggcgg gcaccgggcg tttcctggag gtgatctcgc gcacgcttgg
     4561 caccagcgtc gagcaactcg acagcattac cgaaaatgtc acgccgcatg ccatcacgag
     4621 tatgtgcaca gtgtttgccg aatcagaagt gatcagcctg cgctcagcgg gcgtcgcgcc
     4681 agaagcgatt ctcgccggag tgattaacgc gatggcgcgg aggagtgcca atttcattgc
     4741 tcgtctctcc tgtgaagcgc cgattctgtt tactggtggc gttagtcatt gccagacgtt
     4801 tgcccggatg ctggaatctc acctgggaat gccggtaaat acccatcctg atgcgcaatt
     4861 tgctggcgca attggcgcgg cggtaattgg tcaacgagtg aggacacgcc gatgaaagag
     4921 tttttatttt tatttcactc cacggtcggc gtcatacaaa cccgcaaggc gttgcaggca
     4981 gcgggcatga cttttcgcgt cagcgatatt ccccgtgatt tacgcggcgg ctgcgggtta
     5041 tgcatttggc tgacctgtcc ccccggcgag gaaatacaat gggtgatccc tgggctgact
     5101 gagtcaattt attgtcagca ggatggtgtc tggcgctgca ttgcacatta ccgggtttct
     5161 ccgcgttaag gattcattaa ggtcgctacg tatacttctc gatatcttat tgatttgcct
     5221 taaagggatt accgtgacaa aaagtatttc gttaagtaaa cgcatctccg ttattgtgat
     5281 tctttttgcc attgtggcag tgtgtacttt cttcgtgcaa tcttgcgccc gtaaaagcaa
     5341 tcatgcagca tcatttcaga attaccacgc caccattgac ggcaaagaaa ttgcaggcat
     5401 aacgaataat atctcttcgc tgacctggtc ggcacaaagc aatactcttt ttagcactat
     5461 caacaaacct gccgccattg ttgaaatgac caccaatggt gattttatcc gcacaattcc
     5521 tctggatttt gttaaggatc tcgagacgat cgaatacatt ggcgataatc agtttgtcat
     5581 tagcgatgag cgcgattacg ccatttatgt gatttcactg accccaaact cggaagtaaa
     5641 aatcctcaaa aaaataaaaa tcccgctgca agactcccca accaattgcg gttttgaagg
     5701 gttggcttat tctcgccagg atcacacttt ctggtttttt aaagagaaaa atccgattga
     5761 ggtctacaaa gttaacgggc tgttaagtag caatgaatta cacatcagca aagacgaggc
     5821 actacaacgg caatttactt tagatgatgt gtccggcgca gaatttaacc agcaaaaaaa
     5881 tacgcttctg gtgctgtctc atgaatctcg cgcactccag gaagtaaccc tggttggcga
     5941 ggttatcggt gaaatatcac taaccaaagg tagtcgaggt ttgtcgcata acatcaaaca
     6001 ggccgaaggg attgcgatgg atgcctctgg taatatttat atcgttagcg aaccaaatcg
     6061 tttttatcgc tttaccccta aatcatcgca ttaacaggct ggcatatttc acctttttgt
     6121 tattaacttg ttaacaacaa aaatgacata caaggttaaa catgccttcg tcctcgcatc
     6181 ccgtagaacg cttttctttc agcaccgcgc ttttcgggat gctggttctg accttaggta
     6241 tgggattagg tcgcttcctt tatacgccta tgttgcccgt catgatggcg gaaggggcgt
     6301 tttcatttag ccagctctcg tggattgcca gcggcaacta tgcggggtat ctggctggca
     6361 gtctgctatt ttcgtttggc gcatttcacc agccgtcgcg cctgcgccca tttctgttgg
     6421 tttctgccct ggcgagcggg ttgttgatcc tcgcaatggc atggttgccg ccatttattc
     6481 tggtgttatt gattcgcttc ctggcgggtg tcgccagcgc cggtatgctg atttttggtt
     6541 cgacgctgat tatgcagcac acgcgccatc cttttgtgct ggcagctttg ttttctggcg
     6601 ttggcattgg cattgcacta ggcaacgaat atgttctggc aggcctgcat tttgccttct
     6661 cctcgcaaac gttatggcaa ggcgccgggg cgctttctgg catgatgctg attgcactta
     6721 cgcttttaat gccctcgaaa aaacacgcca tcgcaccaat gccattggca aaaacggagc
     6781 aacagataat gagctggtgg ttactggcta ttctgtacgg ccttgcggga tttggttata
     6841 tcatcgtcgc gacttacctg ccgctcatgg cgaaagatgc aggctcacca ttgttaaccg
     6901 cccatctctg gacgttagtc ggcttatcaa ttgtgccagg ctgctttggc tggctatggg
     6961 cagcaaaacg ttggggggct ttgccttgcc tgacggcgaa tttgctggtg caggctatca
     7021 gtgtgctact tactctcgcc agcgactctc ctctcttgct tatcattagt agtatcggtt
     7081 ttggcggtac ctttatgggc acgacatctc tggtgatgac tatcgcccgc caactgagcg
     7141 ttccgggaaa tctcaatctt ttaggctttg tgacgctcat ttacggcatt ggacaaatcc
     7201 tcggcccggc gctgaccagt atgctcggca atggaacatc ggcgcttgcc ggcgccactc
     7261 tctgcggcgc ggcggcgttg tttattgctg cgcttatcag cacagtacaa ttatttaagc
     7321 tgcaagtggt cacttcatga ggagtgtttc ccttaccact caagaaattc gaccgcaacc
     7381 gttgatgtca gccagacccc gttttccgct tgccagaaag ggaggcctcg tttcgccatt
     7441 tcctgtgctt tgacggttaa aataaccggc gatccgtgtc ttgctcccac tttgcgtgcc
     7501 gttgcttcat cagcagagag atgtacgtag tgacgctcac ctgcaatcag cccctgtttt
     7561 tttatctcgt caaggaaacg gcttgccgta ccgtggtaga gaaactgtgg cggtgttttt
     7621 tcggcaaagg aaatagcgac ctgcgaagtc gaatgcccct gaaccgcgcg gatgcaccta
     7681 ccatcactgg aataactaaa acgcttttta tcgctggtgg caaccacggt atccagcaat
     7741 gcacgggtta accgcttgcc cgctttttgg gcacaaagta tgagcttatc gatatccgcc
     7801 catccttcac ggtccagtac gataccaatc gcctccggtt tgtgtcggag gacaaaactt
     7861 aaaaacttgc tcgtatccgc cagctctttt tcgttgtact ttgccatcgc gttttcgtcc
     7921 ctggattatc tgttttttat ttactatgtc attgtcactg ggtttatgaa ttttctgcaa
     7981 ctcaatcctg cgtagaacct tttccacaca gcgtaatttt accgctatgc aaaaaacgca
     8041 tagtcagaaa catacttgca ttttccccat acccacatgg ctgataaggt ttttcagctc
     8101 caggaatttt cctggctcaa gcaacacaca aacacaaaaa aacaatgggg attgttatga
     8161 cacaacaagg ggatgctgta gccggggagc tggcgacaga aaaagtgggc attaaagggt
     8221 atctggcttt ttttctcacc ataatctttt tttccggtgt tttttccggg acagacagtt
     8281 ggtggcgcgt ttttgatttt tccgttctga acggatcgtt tgggcaactg cctggtgcga
     8341 acggagccac cacctcgttt cgcggcgcgg gtggcgcggg ggctaaagat ggctttctct
     8401 ttgcgctgga gctggcaccg tcagtcattc tctctctggg gattatttca attaccgatg
     8461 gtcttggcgg attacgcgcc gcgcagcagt taatgacgcc agtgctaaaa ccgttgttgg
     8521 gcattccagg gatctgctcg ctggcgctga tcgctaacct gcaaaacacc gatgccgccg
     8581 caggtatgac caaagagctg gcgcaggaag gtgaaattac cgaacgcgat aaagtgattt
     8641 tcgccgcata tcaaaccagc ggtagcgcca ttatcaccaa ctacttctct tccggcgtcg
     8701 cggtgtttgc ctttctgggc atgtccgtaa tcgtaccttt agcagtcatc ctggtgttta
     8761 aatttgtcgg agccaacatt ttgcgcgtct ggcttaactt cgaagaacgt cgcaacccaa
     8821 cgcaaggagc gcaagcatga caactcaggt ccgcaaaaac gtcatggata tgtttatcga
     8881 tggtgcgcgc cgtggcttta ctatcgccac gaccaacctg ctgccaaacg tggtgatggc
     8941 gtttgtcatc attcaggcgc tgaaaattac cggcctgctc gactgggtgg ggcatatctg
     9001 tgaacccgta atggcgctgt ggggattacc cggtgaagcg gcaaccgtcc ttttagccgc
     9061 attaatgagt atgggtggcg cagtcggtgt tgccgccagt ctggcaaccg ctggcgcatt
     9121 aaccggtcac gatgtcaccg ttttactgcc cgcgatgtat ttaatgggca acccggtgca
     9181 aaatgtcggt cgctgcctgg ggacagccga agtgaatgcc aaatattacc cgcatatcat
     9241 tacggtctgc gtgattaacg cattgctgtc gatctgggtt atgcagctta ttgtttaaca
     9301 aggagttacc atgattgatt ataccgcagc cggttttacc ctgctgcagg gagcgcattt
     9361 gtatgcgccg gaagatcggg gaatttgcga tgtcctcgtc gctaacggca aaattatcgc
     9421 cgttgccagt aatatccctt ctgacattgt accggactgc acggttgtcg atctcagcgg
     9481 gcagatcctc tgcccaggtt ttattgatca acacgtccat ttgattggcg gtggcggcga
     9541 agcaggtccc acgacgcgca cgccggaagt ggcgttaagt cgtctgacgg aagcaggcgt
     9601 cacgtcagtg gttggtctgc tgggcaccga ctctatctct cgccacccgg aatccctgct
     9661 cgccaagacc cgtgcgctca atgaagaagg catcagcgcc tggatgctga ccggcgctta
     9721 tcatgtccct tcccgcacta ttacgggttc cgtggaaaaa gacgtggcaa ttatcgatcg
     9781 cgtgattggc gtgaaatgcg ccatctctga tcaccgttct gccgcaccgg acgtttatca
     9841 cctggctaat atggcggcgg aatcccgcgt tggcggtttg ctcggcggta aacctggcgt
     9901 caccgtgttc cacatgggcg acagcaaaaa ggcgttacag cctgtctatg acctgctgga
     9961 aaactgcgat gtgccgatta gcaagttgct gccgacccac gttaaccgca acgttccgtt
    10021 gtttgagcag gcgctggagt tcgcgcgcaa aggcggcacc atcgatatca ccagcagcat
    10081 tgacgaacca gtcgcccctg ccgaaggtat tgcccgcgcc gttcaggcgg gtattccgct
    10141 ggcacgcgtc accctcagct ccgacggcaa cggtagccag ccgttcttcg atgacgaagg
    10201 gaatttaacc catatcggtg ttgccggttt tgaaacgttg ctggaaaccg tgcaggtgct
    10261 ggtcaaagat tatgatttca gtatcagcga tgccctgcgc ccgctcacca gtagcgtagc
    10321 cggtttcctt aacctgagcg ggaaaggcga aattctgcca ggcaatgacg cagacttact
    10381 ggtcatgacg ccagaattac gcattgagca ggtatacgct cgcggcaagc tgatggtcaa
    10441 agacggcaaa gcctgcgtga aaggaacgtt tgaaacggca taaacggttg tcagccgccc
    10501 gaatggacgt agaatgcccg caagggcggc tgacagagta aaacgtaatg gatgactgtg
    10561 gtgcggtttt gcataatatt gaaactaaat ggctttatga ttttctgacc ctggaaaaat
    10621 gccgcaattt ttcccaggca gcagtcagtc gcaacgtctc gcaaccggca ttcagccgcc
    10681 gcatccgtgc gctggaacag gcgattggcg ttgaattgtt taaccgccag gtgacgccgc
    10741 tgcaactctc ggaacaaggt aaaatattcc attcgcagat ccgccatctg ttgcaacagt
    10801 tagagagcaa ccttgcagaa ctccgtggtg gcagcgatta cgcgcaacgc aaaattaaaa
    10861 tagccgctgc acactctctt tccctcgggc tgttaccgtc cattatcagc cagatgccgc
    10921 cgctctttac ctgggcaatt gaagctattg atgtcgatga agcggtcgat aaactgcgtg
    10981 aagggcaaag tgactgtatt ttttcctttc acgacgaaga tttgctggaa gcgccgtttg
    11041 accacattcg cttatttgaa tctcaattgt tccctgtctg cgccagtgac gaacacggag
    11101 aagcactttt taacctcgcg cagccacact ttccgttact gaattacagc cgcaactcct
    11161 acatggggcg attgattaat cgcaccctga cgcgccacag tgagttaagt ttcagcacct
    11221 tttttgtctc ttcgatgagc gagcttttaa agcaggttgc cctcgacggc tgtgggattg
    11281 cctggctgcc ggagtacgcc atccaacaag aaattcgcag cgggcagctc gttgtactga
    11341 atcgggacga actggtgatc ccgattcagg cctatgcata ccgaatgaat acccgcatga
    11401 atccagttgc cgaacgcttc tggcgtgaac tgcgcgagct ggagattgta cttagctgac
    11461 attctcgagc gtcgcactac gccaaccgcc attatcccag accagatcaa acgttagcgg
    11521 ttcatcgtcg tggtacagca ttatctcaag gctgatcgcc agcgtgtggc tgtacgcttc
    11581 tttcagattg acctgcaacg ccgcaatccg tggctcatga aataacagtg cagaacgcag
    11641 ctgacggcaa taccagctct ggagatgttt gtcgctctgg ttatgcactt gccactcggg
    11701 catgcctgaa acaagtaatg acggtaatgc cgaatgagga gaattaaaaa gagtgaggag
    11761 atcgctacat attgatttac gcagcgatga cgttttattg cctgaaattt caggtaaaac
    11821 agcctgacgt gattggcgca tcattttgcg cactcctgac gtttagcaac atatgcggct
    11881 tgccctgaac accgggcacc actgtattga tgctcatttt ccatagccat tcccttttgt
    11941 tcattacatc catgtcagtc tgagacaggc ctgaatcctg tcagcaaagt ataaaacctt
    12001 acttgcctat gaatatctac tgacttttca ggaaaaaagt catgatttga taaaaatcat
    12061 caaactcaaa aaaaaaccgc tagtatattc tgaatattcc gaatagttac gtagatgaaa
    12121 atacttgtca ccaccgctac ttaattccgg tgtgaaatta tttatttccg cata
//
LOCUS       DAJOSY010000122        12024 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJOSY010000122 DAJOSY010000000
VERSION     DAJOSY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.122"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            211..981
                     /locus_tag="OHN27_003695"
     CDS             211..981
                     /locus_tag="OHN27_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCQ2288069.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            972..1445
                     /locus_tag="OHN27_003696"
     CDS             972..1445
                     /locus_tag="OHN27_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCQ2288070.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1552..2091
                     /gene="dnaT"
                     /locus_tag="OHN27_003697"
     CDS             1552..2091
                     /gene="dnaT"
                     /locus_tag="OHN27_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCQ2288071.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2094..2831
                     /gene="dnaC"
                     /locus_tag="OHN27_003698"
     CDS             2094..2831
                     /gene="dnaC"
                     /locus_tag="OHN27_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCQ2288072.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2880..3374
                     /locus_tag="OHN27_003699"
     CDS             2880..3374
                     /locus_tag="OHN27_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCQ2288073.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3628..5919
                     /gene="opgB"
                     /locus_tag="OHN27_003700"
     CDS             3628..5919
                     /gene="opgB"
                     /locus_tag="OHN27_003700"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCQ2288074.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6056..7078)
                     /gene="lgoD"
                     /locus_tag="OHN27_003701"
     CDS             complement(6056..7078)
                     /gene="lgoD"
                     /locus_tag="OHN27_003701"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCQ2288075.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7217..8131
                     /locus_tag="OHN27_003702"
     CDS             7217..8131
                     /locus_tag="OHN27_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCQ2288076.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8346..9707
                     /locus_tag="OHN27_003703"
     CDS             8346..9707
                     /locus_tag="OHN27_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2288077.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9756..11420)
                     /gene="tsr"
                     /locus_tag="OHN27_003704"
     CDS             complement(9756..11420)
                     /gene="tsr"
                     /locus_tag="OHN27_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCQ2288078.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQDDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKNVTPATPRKMAVADSGENWETF"
     gene            complement(11539..11985)
                     /gene="hpaR"
                     /locus_tag="OHN27_003705"
     CDS             complement(11539..11985)
                     /gene="hpaR"
                     /locus_tag="OHN27_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCQ2288079.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQLLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2920 a         3172 c         2934 g         2998 t
ORIGIN      
        1 aacaataaat tcattatagt gataatattc atgtgatagt ttgtttgcgg cgagagataa
       61 ttcgcttttt atcaccgagc ttgtcccgat aaccaggatt aatcagcgca attttgatct
      121 taacctaccg caaaacaacc gtgcttcatg atacaaatgg attctcatta tctgaacaat
      181 acgcagcacg tctatgacaa agggcgagtt atgcaaactg agcaacagcg agccgtaacg
      241 cggctttgta tccagtgtgg attatttctt ttgcaacatg gtgcggaaag cgcgttggtt
      301 gatgagcttt cctcacgact gggtcgggcg ttgggaatgg acagcgtcga aagttctatc
      361 tcttcgaacg ccatagtgct gacaactatt aaagatgggc aatgcctgac atcgacacga
      421 aaaaatcacg atcgcggcat taatatgcat gtggtgactg aagtccagca cattgtgatt
      481 cttgcggagc atcatctgct ggattacaaa ggcgtagaga aacgatttag ccaaattcag
      541 ccattacgtt acccaagatg gctggtagcc ttaatggttg gcctttcttg cgcctgtttc
      601 tgtaaactca ataacggtgg ctgggatggt gccgtcatca ccttctttgc cagtacgacc
      661 gcgatgtata tccgccagct gctggcacaa cgtcatcttc atccacagat caacttttgc
      721 cttaccgctt tcgccgccac caccatttcc ggattgcttt tgcaactccc cactttcagc
      781 gatacaccca ccattgcgat ggccgccagc gttctgctgc tggtgccggg ctttccgttg
      841 attaatgccg tcgccgatat gtttaaaggc cacatcaata ccggactggc acgctgggcg
      901 atcgccagtc tgctgacgct ggctacctgc gtcggcgtgg tgatggcact gacgatttgg
      961 gggctacgcg gatgggtgtg atcgagtttc tgtttgcatt ggcgcaggat atgatcctcg
     1021 ccgccattcc agcggtcggc tttgcgatgg tgttcaacgt tcccgtgcgg gcgttacgct
     1081 ggtgtgcgct gcttggcgcg attggccatg gttcacgaat gatcttaatg accagcgggc
     1141 tgaatattga gtggtcaact tttatggctt ctatgctggt cggcaccatt ggtattcaat
     1201 ggtcgcgctg gtatctggcg catccgaaag tgtttaccgt ggcggccgtt atccctatgt
     1261 tcccgggcat atcggcttat accgcaatga tttcggcggt aaaaatcagc cagttaggtt
     1321 acagcgaacc gttgatgatt accctgttaa ccaactttct tacagcttca tcgattgttg
     1381 gtgcgttatc catcggtctt tccattcctg gattatggtt gtaccgcaag cgccctcgcg
     1441 tataaaattg cctcgcttaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta
     1501 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     1561 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     1621 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     1681 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg
     1741 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     1801 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ctttatccgc
     1861 ctggcggcgc tatggggcgt ggcactaaga gagccggtga ccaccgaaga actggcctca
     1921 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtacagtg gcaacaaaaa
     1981 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2041 aatacggtca gtgaacctga cagccaaatt ccaccaggat tcagagggta acgatgaaaa
     2101 acgttggcga cctgatgcaa cgcctgcaaa agatgatgcc tgcccatatc aaacccgcat
     2161 tcaaaacggg tgaagaacta ctggcctggc aaaaagaaca aggagcgatc cgctccgccg
     2221 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2281 gtccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2341 atgcgttaag caaagcgcgc cagtatgtcg aagagtttga cggcaacatc gccagcttca
     2401 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2461 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     2521 aagatacctt caggaatagc ggtaccagcg aagaacaact gcttaacgat ctgagcaacg
     2581 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     2641 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     2701 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     2761 tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgt gtaacaggta
     2821 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     2881 tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     2941 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3001 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3061 gcagcggaaa ccaggcctta agcgcagata acatgaacaa tgccgcaggc attctgcaat
     3121 actgcgcgaa gcaaaagctg gcgtcggtaa ccgatgccga aaacatcaag aatcaggtgc
     3181 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggacggta
     3241 ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataac atcggaacga
     3301 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3361 acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct
     3421 ctcctgccct tttccctgct cctggacggt tttacccctg ttacacggga agtgaccgta
     3481 atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa
     3541 ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa
     3601 acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc
     3661 gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca
     3721 ttaacggtgc tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt
     3781 actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct
     3841 ggcgtcagca aatacatttt gccgggtatc ggcattgtgc tggggctgac agcggtgttc
     3901 ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc cgcaccattt tggttacagt
     3961 ctgctggcgc tcttactggc gctgggttcg gtggacgcca gcccggcatt tcgtcagata
     4021 acggaactgg tgaaatccca gtcacgcgat ggcgacccgg actttgcggc ttattataaa
     4081 gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt
     4141 ctcgagcgga cctattttga taacgaagct ttcccggatc tcacgcctga actgggcgcg
     4201 ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg
     4261 attgcgggca tggtggcttc tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac
     4321 gcctccgctt ctgtttccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa
     4381 aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat
     4441 gtgttcctga agtcgcatgg cttcgaccac ttatacggct cagaagagct gaaaagcgtg
     4501 gtagccgacc cgcactatcg caacgactgg ggattctacg acgataccgt tctcgatgaa
     4561 gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg
     4621 acagtcgata cccatcaccc ggatggtttt atctcgcgca cctgtaaccg caaaaaatat
     4681 gattttgacg gtaagccgaa tcagtcattc agcgcggtaa gttgcagcca ggagaacatc
     4741 gcgacgttta tcaacaaaat caaagcgtca ccgtggttta aagataccgt catcgtcgtc
     4801 tcttctgacc atttagcgat gaacaacacg gcgtggaaat acctcaataa gcaggatcgc
     4861 aataatctgt tttttgtcat ccgtggcgac aagccgcagc aagagacgct ggcagtgaag
     4921 cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc
     4981 ggacttggtc gtagcagttt atccgggcag tcgatgtcgg aaatcttcct caatatcaaa
     5041 gagaaaacat tggcgtggaa gccagatatc atccgcctgt ggaaattccc taaagagatg
     5101 aaagagttca ccatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt
     5161 ttgccgctgc tgttgcgggt ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac
     5221 tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt
     5281 gaccgttgct acaagatggc acaactctgg gctccggaac tggcactttc caccgactgg
     5341 tgtgtctcgc aggggcaact tggcggtcag caaattgttc agcatgttga caaaacaaca
     5401 tggaagagca agacggcatt taaagatacg gtcatcgaca tggcgcgtta caaaggcaat
     5461 gtcgatacgc tgaagattgt tgataacgat attcgctaca aagccgacag tttcatcttt
     5521 aacgtcgccg gtgcgccaga agaggtgaaa cagtttagcg ggatttcccg tccggagtcg
     5581 tggggccgct ggtccaacgc gcagctgggc gatgaagtaa aaatcgagta caagcatccg
     5641 ctgccgaaga aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt
     5701 cctattccgg tacgcgtagg caatgaagaa caaactcttg tgctgggcaa tgaagtgacc
     5761 accaccacac tgcatttcga taacccaacc gatgccgaca cactggtaat tgtgccgccg
     5821 gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc
     5881 ggcatggtgg aaattaaagt ggtagaacgt gaaggataat tcctgactga tgtgtaggtc
     5941 gcataaggtg cgacctacac gccttctacg gcttacaaca cattattaag aaacaaaaaa
     6001 acagcgccca acacttcccc ggacgctgca tggcaacttt aataatctcg ccttatcaga
     6061 aagtaattac acccttaatc aactcacgat tgttaatcac atcgcgctcg taggtttctg
     6121 ccagcgtggc gaacggatag cgatgggtta acatcatgtc ggcagtgatt ttcccttccg
     6181 ccatcagtcg accgacttta gcgaaatctt ccggcgtggc gttgcggctg cccatcatcg
     6241 tcgtttcttt tttatggaat tccggatcgg agaactgcaa ctcaccttta aacagaccga
     6301 caaataccac cgtgccgccg tgacgaatca gattcacggt gttattcatc gcatgttgat
     6361 tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc aaactgcgcc cgcagctgcg
     6421 cgtcaaaatc ttcggctgac gggtccagca cgggcaactc cagacgcgtt gccacatgtt
     6481 cacggcgcgc tggactggta tccgccacca ccacctgtgc gccatcagct ttagcgattg
     6541 ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag cacctgctcg ccgggagcaa
     6601 tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg ttcaatcaat gcggccgcct
     6661 gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg cacgctcaaa tactcactaa
     6721 aaccgccatc ctgatgcacg ccaatgactg aaattttttc acagcaattg gtacgcccgc
     6781 ttttacacgc cgggcattgc tgacaggcaa cataagggat cacagcaact tgctgaccat
     6841 ttttaagatc agcaatattt ttacccagcc caacaatctc cccacatatt tcatggccta
     6901 aaacacgtgg ataactaaaa aatggttgat ttccacccca ggcatgaata tcggtaccgc
     6961 aaatcccgac agacttaatt tttattaatg cttcattgtc acccggaata ggtatctcgc
     7021 gttgtttcca gactaattct ttcggctgct ggcaaattaa aacattcatc gtagacatga
     7081 ttcggctctc cattttttgt tgccttagat atcaatgaaa atgaaaaatg gcagcggaac
     7141 ggaaaatctg ttttgtgaaa acacacgcat aaaaatgttt taaatcgggt tttaatggac
     7201 tcacaaggag gaaaatatga gtcgttcaca aaatttacgc cacaatgtga ttaaccaggt
     7261 cattgatgat atggcgcggg gtcatatccc ttccccactt ccatcgcaaa gtgcgctggc
     7321 ggaaatgtac aacatcagcc gcactacagt gcgtcatatt ctcagccact tacgcgaatg
     7381 cggcgtctta acgcaggttg gaaacgacta tgtgattgcg cgtaaacctg accatgatga
     7441 cggtttcgcc tgtactaccg cttcaatgag tgaacaaaac aaagtgtttg agcaggcttt
     7501 tttcacgatg ataaaccagc gccagttacg ccctggggaa actttttctg aactgcaact
     7561 ggctcgggcc gcgggtgtca gcccggttgt agtaagagaa tatcttttaa aattcggccg
     7621 ttataatctt attcaaagcg aaaagcgcgg ccagtggagc atgaagcaat tcgatcaatc
     7681 ctacgccgag cagctgtttg aactacgcga aatgctcgaa acacattcac ttcagcactt
     7741 tctcaacctg cctgatcatg atccccgctg gctgcaggcg aaaaccatgc tcgaacgtca
     7801 tcggctactg cgtgacaaca tcggcaatag ctttcgcatg ttctcgcaac ttgacagaga
     7861 ttttcactcc ttactgcttt cagctgccga caatatcttt tttgatcaat ctcttgagat
     7921 catctcagtc atctttcatt ttcactatca atgggacgaa agcgatctta aacagcgcaa
     7981 tatcattgcc gtcgatgagc atatgaccat tctcagcgct ctgatttgcc gcagtgacct
     8041 tgatgccacc cttgcgctcc gtaaccattt gaattcagct aaacaatcaa tgattcgatc
     8101 cattaatgag aacacgcgtt atgctcatta agtacggatt aaaaacagca aactattagc
     8161 gggtgcacgg ttttatcaaa agataaatac cgggaaattc ccgcttacct atgctcacaa
     8221 tcagtcgact aaaaatgttt ttgtgagcgg tagtaaagtc ctaaaacttt aacctgccgt
     8281 taatgtcaac cttgcgtaat gcacaattac gtaaacaatt aaaattataa atacaggagt
     8341 ccggcgtgga aaaagaaaat atcaccctcg atccgcgttc ttcatttact ccatcttcgt
     8401 cggcagatat tcccgtgcca ccagatggat tagttcaacg cagtacccga attaaacgca
     8461 ttcaaaccac cgccatgttg ttattatttt ttgcggcggt aatcaattat ctcgaccgca
     8521 gttcgctgtc ggtagcaaat ttaacgattc gtgaagaatt gggattaagt gccaccgaaa
     8581 tcggcgcttt gctctccgtg ttttcactcg cttacgggat tgcgcaactt ccttgcggcc
     8641 cactattgga tcgtaaaggc ccacgcctga tgctgggact ggggatgttc ttctggtcac
     8701 tgttccaggc catgtctggc atggtgcaca gctttacgca gttcgtgttg gtgcgtatcg
     8761 gtatggggat tggtgaagcg ccgatgaacc catgtggcgt aaaagtcatt aacgactggt
     8821 tcaacatcaa agagcgcgga cgcccgatgg gcttcttcaa cgcagcttct accattggcg
     8881 ttgccgtaag cccaccgatt ctggcggcga tgatgctggt gatgggctgg cgtgggatgt
     8941 ttattaccat tggtgtgctg gggatttttc tcgccattgg ctggtatatg ctctatcgca
     9001 accgcgagca cgtagaactg actgccgttg aacaagctta tctcaatgca ggtagcgtca
     9061 atgcccgccg agatccgctc agttttgccg aatggcgcag cctgttccgt aaccgcacaa
     9121 tgtggggaat gatgctcgga ttcagtggca tcaactacac tgcgtggctg tatctggcct
     9181 ggcttcctgg ttacctgcaa acagcctata acctggattt aaaaagcaca gggttgatgg
     9241 cggctatccc tttcctgttt ggggctgccg ggatgctggt caacggttac gttactgact
     9301 ggctggtcaa agggggaatg gctccgatta aaagccgtaa gatctgcatt attgccggga
     9361 tgttctgttc tgccgccttt acgctggtcg taccgcaagc gacaacatcc atgacggcgg
     9421 ttctgctgat tggcatggca ctgttctgta ttcactttgc cggaacatcc tgctggggct
     9481 tgatccacgt cgcagttgct tctcgcatga cggcgtcggt gggcagtatc cagaactttg
     9541 ccagcttcat ctgcgcctct tttgcgccga tcattactgg ttttattgtt gataccaccc
     9601 actcattccg tctggcacta atcatctgcg gttgcgtcac cgcagcggga gcactggcgt
     9661 acatcttcct ggttcgtcag ccgatcaacg acccgcgtaa agattaaact ccctatgccc
     9721 gataacattt tgcttatcgg gcatttttat ggcgattaaa acgtttccca gttctccccg
     9781 ctatctgcca ctgccatttt acgcggcgta gctggcgtca cgttttttac cacagccgat
     9841 gtttcacgct gctgttgctg ctgttgctga atccggaaca ctgccacagc ttcggtcagg
     9901 cgactggcct gctcttccag cgcggcggcg gcagcggcag attcttccac cagcgcagcg
     9961 ttctgttgag ttacccggtc catctcagca accgctaagc caacctgatc gataccacgg
    10021 ctctgctcat cagaagcaga agcaatttcg cccataatgt ccgtcacgcg ggtcacggca
    10081 ctgacaatct ccgccattgt ttcccctgcg ctttcgacca gcgtagagcc aacatctact
    10141 ttccccaccg agtcttcaat caggctttta atttcacgag ccgcctgagc gctgcgctga
    10201 gccagattac gcacttcacc cgcgaccacc gcaaaaccgc gcccttgctc acccgcgcgc
    10261 gctgcttcaa ccgccgcgtt caaagccaga atattggtct ggaaggcaat gccgtcaatt
    10321 acgctgataa tatcggcgat tttctgcgaa ctggtggaga tatcgcgcat ggtctgtact
    10381 acgttatcca ccactttgcc gccgcgttgc gccgtttcag aagcacttaa cgccagatgg
    10441 ctggcctgac gcgcattctc ggcgttctgt ttcaccgttg cggtcagttg ctccatgctg
    10501 gctgccgtct cttccagcga agcggcctgt tgctcggtgc gcgaagagag atcgttattg
    10561 ccggtggcga tttcgctggc accgctatag atggcattgg ccccgttgcg cacatcaccg
    10621 acggtacgca tcagctctcc ctgcatatgg cgcaaactct ctgccagttg ccccatctca
    10681 ttagagccat ccacctcaat cggtttcacc agatcgccgc ctgcaatatg acgaatgctg
    10741 tcaatcaggc gattcattgg cgctaccagc gaggctttaa taccgaacca gacggcgaag
    10801 atgaccgcca gtacgacgat catcacgccc accagaatcc acatcgcctg gctgtaggag
    10861 gcattgttat cgctgacggc gatatcatag agccgatcgt cttgctccat gtaagccaca
    10921 tactgcttct cgaaaccgtc ctgatatccc tgggtcggct gatcaaagaa ctcgttgatt
    10981 ttgcctgcac ctaatagttg gatcagctcc gccagcgcat tgtgataaat atcgtaatta
    11041 cgtttgatct ctgccgctgc ggcggtgctc tgacgcgggt cacgcggcaa cgcttcgtaa
    11101 tccgcccagt ttttttccgc ctgtttcagc gaaatactgg cactctgcat cagctcagca
    11161 acggttgaac cgctaccaat attattctga tccatcatgt agcggatacc cgcgcggttg
    11221 agggtgttac gcgtctgcaa caacgcgacc cagctgccat tcagcgtgga ttgctgctgg
    11281 cgaatggttt gtaaaacagt gaaattttct ttgtcattct ttaaggcatt aaagaacaga
    11341 ccgcctgatg tcagttgtaa aaggccaaaa acggccaaaa ccagcagtaa gctggtcacg
    11401 atcttgatac gttttaacat ggtttctctt tcctgtggac cgatgcaaga ttttcggcct
    11461 ggaaagcaaa aactttatga aaaggtgtaa cccgccatcc atggaaaaac caggggctgg
    11521 ttatcagata ctaaaaagtt attcattatc gcccgggatg tcttcttgtc gggaattgcc
    11581 cagagcaata aattcctcta acagatgggt taacagttgc attttctcgg cagtaaactg
    11641 agcttcaatc tgccgatagg cttcttcaat ctgtgtctga gcgcggttat acagcgcctg
    11701 cccctctttg gtcagcgaga tatagagctt ccgctgatca ttgatcggct ttaatcgcaa
    11761 cactaaaccg tcgcgttcca tccgcgtaag gatcccggtc aggcttgggc gcaaaatgca
    11821 ggcacgatac gccagatcgt gaaaatccat tgatgggctt tccgccagaa tacgcacgat
    11881 gcgccactgt tgctcagtca aattatgccg tttaacgatt gggcgaaaat aactcatcgc
    11941 cgcttctcgc gcctggagca acgcaatggt tagagagtcg tgcattatct ttcccctgaa
    12001 aaattcaaaa tcattaatat agaa
//
LOCUS       DAJOSY010000123        11775 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJOSY010000123 DAJOSY010000000
VERSION     DAJOSY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.123"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(34..315)
                     /locus_tag="OHN27_003706"
     CDS             complement(34..315)
                     /locus_tag="OHN27_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCQ2288080.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(578..1687)
                     /gene="apbC"
                     /locus_tag="OHN27_003707"
     CDS             complement(578..1687)
                     /gene="apbC"
                     /locus_tag="OHN27_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCQ2288081.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGVKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLITDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLRDDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1819..3852
                     /gene="metG"
                     /locus_tag="OHN27_003708"
     CDS             1819..3852
                     /gene="metG"
                     /locus_tag="OHN27_003708"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCQ2288082.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3993..7787
                     /locus_tag="OHN27_003709"
     CDS             3993..7787
                     /locus_tag="OHN27_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HCQ2288083.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQVKSFAD
                     AAAAEKAGLKLIAEKVKKGYVAQAKDNSLQPSQTVTGYLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKKATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAAMAALTELLAQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHKPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7797..11429
                     /locus_tag="OHN27_003710"
     CDS             7797..11429
                     /locus_tag="OHN27_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCQ2288084.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRKWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAALACIAQ
                     NDNQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESRWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVATDPKAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQAQSVVKRYLSASPKPVIDSTDNSGLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQCDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11491..>11775
                     /locus_tag="OHN27_003711"
     CDS             11491..>11775
                     /locus_tag="OHN27_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288085.1"
                     /translation="MIVQKELVAIYDYEVPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRE"
BASE COUNT         3078 a         2998 c         2915 g         2784 t
ORIGIN      
        1 ctttctaaat gcaattaaaa tcaggttgcc aaattaatcg tagcgattta acacgattcg
       61 acgtttgcta tcgctacctg caacaaccac ttctgtcgtt acacctttga cgggcgaaga
      121 aaacagatga cgggtgtcaa cattatacgt taactgacca caatgctgtt gaggttgacc
      181 attaaccata gccatactgc atgttggggg actgatcctg ccattaatcg ctaatgtggc
      241 agtctctgaa gagaatgcgg gagaagccaa tccataggca agaaaaataa ttcctgacag
      301 ccaatactta ttcattgaac gttatccctg tagtaaaggt tatgcctgga tagaatgagt
      361 gcataacaaa ctatagctgt acatccacta cacagccacg aaggatgata atgaagcatt
      421 gcctgtatga tcaatcgact ttgtagagtt tcggacgaag gtccgcagaa tattcgcagt
      481 attaaataag tgttcattaa tgcggcaaaa actaatacac cgcatcaatg taacatctct
      541 gcggtgtatg aagaataacc agaatggtta ataggcgtta gaccgcgcgg aaggaaatct
      601 cgcctggaat gacttcaccc tgccagtaga gctgagctgc aacgcggtca gccagctggc
      661 ggtagatagc ggtaaattcg ctctccggac ggctgataac cgttggcgtg cctttatcga
      721 gatcatcacg taaggaaatg tgtaacggca tctgccccag cagctgtgtg tggtattttt
      781 cagccagttt ctctgcacca ccggtaccga aaatcggttc atggtgacca cagttactgc
      841 aaatatgcac actcatgttc tcgacaatac caagcaccgg aacctcgact ttctcgaaca
      901 tcacaatgcc ttttttcgca tcgatcaacg cgatgtcctg cggggtggtg accacgactg
      961 caccagttac tggaatgttt tgcgccagcg tcaattgaat gtcaccggtg cctggcggca
     1021 tatcaagcac cagatagtcg agatccggcc acaaggtttc ctgcaacatc tgcattaacg
     1081 ccttgctcgc catcggtcca cgccacacca tcgcattgtc gtcagtgatc agataaccaa
     1141 ttgaattggt tgccaggcca tgagacataa tcggtgccat atgggtaccg tccggcgaag
     1201 ttggacgctg attttctgcg cccagcatgg tgggaattga tgggccgtag atatcggcgt
     1261 ccagaatgcc gactttcgcc ccttccgccg ccagtgccag cgccaggttt accgccgtgg
     1321 aggatttacc cacgccgcct ttaccggaac tgaccgcaat gatgttcttc acaccattaa
     1381 tacccggctg attcttcacg cgttttaagg tggcgatatt gtgcgacagt ttccagtcga
     1441 tagctttcac cccggtgata cgcagcagtt cggcgctaca ttgctctttt aactcttcaa
     1501 acgcgctatg ccagacgaat ggcataacca gttccacatg caacgtgtcg tccatccagg
     1561 cgacatggtg caacgctttc agcgtggtga ggttatgctt cagggttggg tgctgaaaat
     1621 tggccagtgt cccggcgacc attgcgcgca aggcttctgg tgatttggcc tgggattgtt
     1681 cgttcatccc gactcctttt ttgtatagat aaaccatcag ctgatagttt acctgaagaa
     1741 tatagagaag tacttactta acattttccc atttggtact atctaacccc ttttcactat
     1801 taagaagtaa tgcctactat gactcaagtc gcgaagaaaa ttctggtgac gtgcgcactg
     1861 ccgtacgcta acggctcaat ccacctcggc catatgctgg agcacatcca ggctgatgtc
     1921 tgggtccgtt accagcgaat gcgcggccac gaggtcaact tcatctgcgc cgacgatgcc
     1981 cacggtacac cgatcatgct gaaagctcag cagcttggta tcaccccgga gcagatgatt
     2041 ggcgaaatga gtcaggagca tcagactgat ttcgcaggct ttaacatcag ctatgacaac
     2101 tatcactcga cgcacagcga agagaaccgc cagttgtcag aacttatcta ctctcgcctg
     2161 aaagagaacg gttttattaa aaaccgcacc atctctcagc tgtacgatcc ggaaaaaggc
     2221 atgttcctgc cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc cccggatcaa
     2281 tacggcgata actgcgaagt ctgcggcgcg acctacagcc cgactgaatt gatcgagccg
     2341 aaatcggtgg tttctggcgc tacgccggta atgcgtgatt ctgaacactt cttctttgat
     2401 ctgccctctt tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt gcaggagcag
     2461 gtggcaaata aaatgcagga gtggtttgaa tccggcctgc aacagtggga tatctcccgc
     2521 gacgcgccat actttggttt tgaaattccg aacgcgccgg gcaaatattt ctacgtctgg
     2581 ctggacgcac cgattggcta catgggttct ttcaagaatc tgtgcgacaa gcgcggcgac
     2641 agcgtaagct tcgatgaata ctggaagaaa gactccaccg ccgagctgta ccacttcatc
     2701 ggtaaagata ttgtttactt ccacagcctg ttctggcctg ccatgctgga aggcagcaac
     2761 ttccgcaagc cgaccaacct gtttgttcat ggctatgtga cggtgaacgg cgcaaagatg
     2821 tccaagtctc gcggcacctt tattaaagcc agcacctggc tgaatcattt tgacgctgac
     2881 agcctgcgtt actactacac tgcgaaactc tcttcgcgca ttgatgatat cgatctcaac
     2941 ctggaggatt tcgttcagcg tgtgaatgcc gatatcgtga acaaagtggt taacctggcc
     3001 tctcgtaatg cgggctttat caacaagcgt tttgacggcg tgctggcaag cgaactggct
     3061 gacccgcagt tgtacaaaac cttcactgat gccgctgaag tgattggtga agcgtgggaa
     3121 agccgcgaat ttggtaaagc agtgcgcgaa atcatggcgc tggctgatct ggctaaccgc
     3181 tatgtcgacg agcaggctcc gtgggtggtg gcaaaacagg aaggccgcga tgccgacctg
     3241 caggcaattt gctcaatggg cataaacctg ttccgcgtgc tgatgactta cctgaagccg
     3301 gtactgccga aactgaccga gcgcgcagaa gcattcctca atacagaact gacctgggat
     3361 ggtatccagc aaccgctgct gggccacaaa gtgaatccgt tcaaagcgct gtataaccgc
     3421 atcgatatga agcaggttga agcactggtg gaagcgtcta aagaagaagt gaaagcagct
     3481 gccgcgccag ttactggccc actggcagat gatccgattc aggaaaccat cacctttgac
     3541 gacttcgcaa aagttgacct gcgcgtggcg ctgatcgaaa acgcagagtt tgttgaaggt
     3601 tctgacaaac tgctgcgcct gacactggat ctcggcggtg aaaaacgcaa tgtcttctcc
     3661 ggtattcgtt ctgcctaccc ggatccgcag gcactgatcg ggcgtcacac cattatggtg
     3721 gccaacctgg caccgcgtaa aatgcgcttt ggtatctccg aaggcatggt aatggctgct
     3781 ggtcctggcg ggaaagatat cttcctgtta agcccggatg ctggtgctaa accaggccat
     3841 caggtgaaat aatccccctt taaggcgctg catcgacagc gccttttctt tataaattcc
     3901 taaagttgtt ttcttgcgat tttgtctctc tctaacccgc ataaatactg gtagcatctg
     3961 cattcaactg gataaaatta cagggatgca gaatgagaca ctttatctat caggacgaaa
     4021 aatcacataa attctggacg gtggagcaac agggaaacga gttgcatatc agttggggaa
     4081 aagttggcac caaagggcaa agtcaggtaa aaagttttgc ggatgcagca gcggcggaaa
     4141 aagcgggact gaagctgatt gcggagaagg tgaagaaggg gtatgtggcg caagcgaaag
     4201 ataattcttt gcaaccttcc caaacggtaa cgggctatct caaggtagcg gacttatcca
     4261 ccattattca ggaacaaccc tctttcgtag cagaaacccg tgcggctgac aaaaatacag
     4321 atgctgtttt accgtggctg gcgaaagata ttgctgtcgt ttttccgccc gaagttgtac
     4381 acaccacgtt aagccatcgc cgctttcccg gagttcctgt tcagcaagca gacaaattga
     4441 cccaattacg tcgcttagcc tgtagcgtgt cgcaacggga taataaaaaa gccacatttg
     4501 atttcagcac ctgttcttta gaatggcaaa acaccgtcgc ccaggcgatc agtcagatcg
     4561 acggcctgaa aacaacacag ctaccatcac cagtaatggc tgtactcacg gcacttgaaa
     4621 tgaaatgcac aagatataaa gtgcgtgagg atgttatgga tcagatcgtc caggaaggcg
     4681 gtctggaata tgctactgat gtaataatac accttcaaca gattgatatt aaatgggatt
     4741 atgcgaataa tgtcattatt attctgccgt ctggcattgc acctgactac ttggagcaat
     4801 attccagatt tgaattacgc ctacgtaaac atttatcact ggcggaagag tctctctggc
     4861 aaaaatgtgc acaaaaactt attgccgcaa ttccacatat tccagaatgg cggcaaccat
     4921 taattgcttt gttattaccc gaaaaaccag aaattgcaca tgaaattgcc cagcgtctac
     4981 tggggcaaaa aaaattaccc tcgcttgagt ggttaaaaat agtggcgact gatgagcaca
     5041 ttcttgcctc attagaaaaa tatcacgagc catatgccat ttttgatgat tactattgtg
     5101 gtgcgatatg gtcagccacc gtattacagg agcaaggtgt tgcagccctg ccccgatttg
     5161 ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat catccgttcg
     5221 cattaacact gcttatacgt gtagccgggc atactaaacg ctgtcacgat cgaatgacga
     5281 aagcctgcgc tgcgttccca catgcagcaa tggcggcact gacggaactt cttgcgcaaa
     5341 aagaagagaa cagttggcgc attatgctaa tgacaatgct tatctcacaa ccagcactgg
     5401 cagaacaggt cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc
     5461 aacaactgac acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct
     5521 ccccaccatg gctttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat
     5581 taggcatcga gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat
     5641 atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg
     5701 caaggatggg ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag
     5761 ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt
     5821 cacctaccgg gcattattgg cagttgggga ttttgacaac attgccgctg gagaaagcag
     5881 taaaagcatg gaatgccctt accctatctc cacataccga taccgaatac gccatgttac
     5941 attttggact caaagggtta cctgggttag taaactcact tgcacgctat ccacaagaag
     6001 ccttgcccat cacgaattac ttcgcagcga gtgagctggc tcctgccgtc gcccgtgcct
     6061 tcaacaaact gaaaacacta cgcgaaaacg cccgtagctg gctgttgaaa tacccggaac
     6121 atgcccttac cggcctgctg cctgcggcgc tcggcaaagc cggtgaagca caggataacg
     6181 cccgcgctgc cttgcgtatg cttaccgaaa acggtcataa gccattactg caagaaatcg
     6241 cccgacgtta taaccagccg gaagtaaccg atgcggtgaa cgctctgctt gcgctcgatc
     6301 ccttagataa tcacccgaca aaaatcccca ctcttccggc cttttatcag ccatcgctct
     6361 ggacgcgccc ggtattaaaa gcaaatgccc aatcactgcc agatagcgcc ctcctccacc
     6421 tcggtgaaat gctccgcttc cctcaggaag aggctctgta tccgggatta ttgcaggtga
     6481 aagacgtctg ttccgccgac tcactggcgg gatttgcctg ggatctgttt accgcctggc
     6541 agaccgctgg cgcgccgtcg aaagagagtt gggcgttcac tgcgttaggc gttctcggta
     6601 acgatgacac cgcccgcaaa ctgacgccat taatacgcgc ctggcctggt gaatcccagc
     6661 ataaacgcgc caccgttggg ttggatattc tcgctgctat cggtagtgat atcgccctta
     6721 tgcagcttaa cggcatcgcc cagaaactga aattcaaagc attacaggag cgggcaaaag
     6781 aaaaaattgc cgacattgcc gagagccgcg aactcacggt ggcggagctt gaagatcggt
     6841 tagcaccgga tctcggtctg gatgataacg gttcgctgct gctggatttt ggcccacggc
     6901 agttcaccgt cagctttgat gaaaccttaa aaccgtttgt gcgtgatgtt tccggcagcc
     6961 gcctgaaaga cctgcccaaa ccgaacaaaa gcgatgatga aacgcgggcg aacgatgcgg
     7021 ttaaccgcta caaattgctg aaaaaagatg cgcgtaccat cgccgcccag caggtagcaa
     7081 ggctggaatc cgccatgtgc ctgcgccgcc gctggtcgct ggaaaacttc cagctcttcc
     7141 tggttgagca tccgctggtt cgtcacttaa cccgccgtct gatttggggc gtttatagcg
     7201 ccgaaaacca gctactggct tgctttcgcg tagcagaaga taacagctac agcaccgctg
     7261 acgatgatct tttcaccctg ccggaaggcg atatctctat cggcactcct cacgttctgg
     7321 aaatatcacc aacggatgct gccgcctttg gtcagctttt tgccgactac gaactgctac
     7381 caccgttccg ccagctcgac cgtaacagct acgccctgac agaagccgag cgcaatgcca
     7441 gtgaactgac ccgctgggca ggcagaaaat gcccgagtgg tcgggtcatg gggctggcga
     7501 ataaaggctg gataaagggc gaaccacagg atggaggctg gatcggatgg atgatcaaac
     7561 ctttgggtcg ctggtcgtta atcatggaaa tcgacgaagg ttttgcggtg ggtatgtcac
     7621 cagccgaact tagcgctgag cagctcttaa gcaagctgtg gttatgggaa ggcaaagcgg
     7681 aaagctatgg ctgggggagt aattcaacac aggaagcgca gttctccgta ctcgatgcca
     7741 tcaccgccag cgagctaatt aacgatattg aagccctgtt tgaataagga aagagcatgg
     7801 acaaggaatt accgtggctg gcggataacg cccaactgga actgaaatat aaaaaaggca
     7861 aaacaccact cagtcatcgc aaatggccgg gcgaaccggt gcctgttatc actgaaagca
     7921 tcattcagac actgggtgat gaattgctac aaaaagctga gaagaaaaaa aacattgtct
     7981 ggcgttatga aaatttttca ctggagtggc agtccgccat tacgcaggcc atcaacttga
     8041 tcggcgaaca caaaccctca gtcccggccc gcacaatggc ggcactagcc tgtatcgcgc
     8101 aaaatgacaa ccaacagttg ctcgacgaaa tcgtccaaca agaggggctg gaatatgcga
     8161 ctgaggtggt gattgcacgc cagtttattg cacggtgtta tgagagtgat cctctggtag
     8221 tcacattgca gtatcagaac gaggactatg gctatggtta tcgctcagaa acctataacg
     8281 aattcgatct ccgactgcgt aaacacctct ctctggcaga ggaaagccgc tggcagcgtt
     8341 gcgccgacaa actcattgcc gcactgccag gaataaccaa agttcgccgc ccttttattg
     8401 cgctgatcct ccccgaaaaa ccagaaatcg ccaatgagtt ggtaagcctt gaatgcccgc
     8461 ggactcattt tcattctaag gagtggttaa aagttgttgc tactgacccc aaagcggtga
     8521 gaaaactcga acgctactgg agccaggata tatttagcga tcgagaagcc agctacatgt
     8581 cgcatgaaaa ccacttcggc tacgcggcct gcgccgccct tttgcgcgaa caaggactgg
     8641 cagccgttcc gcgcctcgcg atatatgccc ataaagaaga ttgcggcagt ctgctggtac
     8701 aaattaacca tccgcaagtc atccgcacct tgctgttagt tgctgataaa aacaaaccca
     8761 gcctgcaacg tgtggctaaa tacagtaaaa acttccccca tgcgacactc gccgcactgg
     8821 cagaactgct ggcgttaaaa gaaccaccag cccgcccagg ttatccaatc atcgaagaca
     8881 aaaagctgcc tgcacagcaa aaagctcgag atgaatactg gcgtacgctg ttacaaacgc
     8941 tgatagcatc gcagccacaa ctagcagaaa gggtgatgcc gtggttaagt actcaagccc
     9001 agtcagtggt gaagcgttat ttatcggcat cgccaaaacc ggttattgat agcaccgata
     9061 acagcggtct gcctgaaatg ctcgtttcac caccgtggcg tagtaagaaa aaaatgacag
     9121 ctccacgtct tgatttggca ccgctcgaat taactccgca agtttactgg caaccaggcg
     9181 aacaagagag gcttgccgcc actgagtctg cccgttattt cagcacggaa tctcttgcgg
     9241 aacgcatgga acaaaaaagt ggacgagttg tattacagga actgggtttt ggggatgatg
     9301 tatggctgtt tctgaattat atactccccg gaaaactgga tgctgcacgc aattcactca
     9361 ttgttcagtg gcattactac caggggcggg ttgaagagat cctgaatggc tggaactccc
     9421 cggaagcaca attagcagaa caggcgctcc gcagcggtca catagaagcg ttaattaaca
     9481 tatgggaaaa tgacaactac tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt
     9541 tattggcaca gttgccgcgt gagatggctt tgaccttctg gctgcgtatc aatgagaaaa
     9601 agcatctgtt cgcgggtgag gactattttc tcagtatcct cggattggat gcgctaccag
     9661 gtctgctgtt ggctttttca catcgtccaa aagaaacatt tccgttaatt ttaaattttg
     9721 gcgcaacaga actggcgctg cctgttgccc gcgtctggcg acgtttcgcg gcgcagtgtg
     9781 atctggctcg ccagtggatt ttacaatggc cggaacatac ggctagtgca cttatccctc
     9841 ttgtctttac caaacccagc gataatagcg aagccgcatt acttgccctg cgtttactgt
     9901 acgaacaggg acatggcgaa ttgctacaaa ccgtggcaaa ccgctggcag cgtacagatg
     9961 tatggtctgc cctggagcag ttgcttaaac agggtccaat ggacatttac ccggcacgca
    10021 ttccaaaagc ccctgatttc tggcatccgg caatgtggtc caggcctcga cttatcacta
    10081 ataatcagcc tgttaccggt gacgctctgg aaattatcgg cgaaatgctg cgctttaccc
    10141 aggggggacg tttttatagc gggctggaac aactgaaaac gttctgccag ccacaaacgc
    10201 tggcagcttt tgcctgggat ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag
    10261 acaactgggc atttctggcg ttaagtctct ttggtgacga aagcacggca cgggatctga
    10321 cgacacagat cctcgcctgg ccacaagaag gcaaatctgc ccgtgctgtc agcggcctga
    10381 acatccttac cctgatgaat aatgatatgg cgctgataca gctgcatcat atatcgcaac
    10441 gggctaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggta gtcgcagaaa
    10501 atcgcgggct aagccaggaa gagctagcgg acagattagt cccaaccctg ggccttgatg
    10561 atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc ttcgatgaaa
    10621 acctcaaccc ggttatcttt gatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc
    10681 gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa
    10741 aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca
    10801 cccgacgctg gtcgctggca gattttcatt ctctgtttgt taatcatccc tttacccgtc
    10861 tggttaccca gcgattaata tggggggttt atccggcaaa tgaaccgcgt tgtttactca
    10921 aagcctttcg tgtggccgca gagggggagt tctgcaatgc gcaagatgag ccaattgacc
    10981 tgcctgcgga cgctctgatt ggcattgccc acccgttaga aatgacagta gaaatgcgca
    11041 gtgaatttgc acagcttttt gccgattacg aaattatgcc gccttttcgc cagttgtcgc
    11101 gccgcacggt gctgctcaca cctgacgagt caaccagtaa cagcctgact cgctgggaag
    11161 gtaaatccgc taccgttggg cagcttatgg gaatgcgata caaaggctgg gagtcaggct
    11221 atgaggacgc atttgtctat gacctgggcg agtaccggct ggtccttaag ttttcacccg
    11281 gttttaacca ctacaatgtt gatagcaaag cgttaatgag cttccgttct cttcgagtgt
    11341 accgtgacaa taaatccgtc acttttgccg aacttgatgt gtttgatttg agtgaggcgt
    11401 taagcgcacc tgacgtcatt ttccattaaa aacacagcgg gcagtgatgc aactgcccgt
    11461 tatcaacacg acttaccagc ggggaaagcg atgatcgtgc aaaaagagct ggttgctatt
    11521 tacgattatg aggtccctgt acctgaaaat ccgttttcct tcagacttga gatccataaa
    11581 tgctctgaat tatttacagg ttccgtctat cgactggagc gattccggct acgtccaaca
    11641 tttcatcaac gtgatcgaga agatgctgac ccgctaataa atgatgcgtt gatttatata
    11701 agagatgagt gtattgatga gcggaaatta cgaggtgaat cacctgaaac tgtaatagca
    11761 atttttaatc gtgaa
//
LOCUS       DAJOSY010000124        11614 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJOSY010000124 DAJOSY010000000
VERSION     DAJOSY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.124"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            144..959
                     /gene="gspC"
                     /locus_tag="OHN27_003712"
     CDS             144..959
                     /gene="gspC"
                     /locus_tag="OHN27_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HCQ2288086.1"
                     /translation="MPTLRFPFHLANHNKDAAINILIIFISIGSIIFNVNYFHTTIVK
                     NGQIINQPTNAFQSDFSLAALWRNENHAGVKDANPVAVNQETPKLSIALNGIVLTSND
                     ETSFVLINEGSEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDI
                     IKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVGELTGMNGLRHERTQ"
     gene            943..2895
                     /gene="gspD"
                     /locus_tag="OHN27_003713"
     CDS             943..2895
                     /gene="gspD"
                     /locus_tag="OHN27_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HCQ2288087.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNARKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHAPAPSSR"
     gene            2905..4385
                     /gene="gspE"
                     /locus_tag="OHN27_003714"
                     /pseudo
     CDS             2905..4385
                     /gene="gspE"
                     /locus_tag="OHN27_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            4382..5578
                     /gene="gspF"
                     /locus_tag="OHN27_003715"
     CDS             4382..5578
                     /gene="gspF"
                     /locus_tag="OHN27_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCQ2288088.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5588..6025
                     /gene="gspG"
                     /locus_tag="OHN27_003716"
     CDS             5588..6025
                     /gene="gspG"
                     /locus_tag="OHN27_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCQ2288089.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            6033..6542
                     /gene="gspH"
                     /locus_tag="OHN27_003717"
     CDS             6033..6542
                     /gene="gspH"
                     /locus_tag="OHN27_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCQ2288090.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6539..6916
                     /gene="gspI"
                     /locus_tag="OHN27_003718"
     CDS             6539..6916
                     /gene="gspI"
                     /locus_tag="OHN27_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCQ2288091.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRNAIERMRNET
                     LALWIADNQLQSQDSFGEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSIDNKSGQAQDD"
     gene            6909..7496
                     /gene="gspJ"
                     /locus_tag="OHN27_003719"
     CDS             6909..7496
                     /gene="gspJ"
                     /locus_tag="OHN27_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCQ2288092.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     QTQQYGGLQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7489..8472
                     /gene="gspK"
                     /locus_tag="OHN27_003720"
     CDS             7489..8472
                     /gene="gspK"
                     /locus_tag="OHN27_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCQ2288093.1"
                     /translation="MNNEQRGVALLIVLMLLALMTALAADMTLSFHSQLQRTRQVNHH
                     LQRQYDIELAKKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLEDGNTVKWQLRDAQH
                     CFNLNALAKISDDPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDSFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKRHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8487..9650
                     /gene="gspL"
                     /locus_tag="OHN27_003721"
     CDS             8487..9650
                     /gene="gspL"
                     /locus_tag="OHN27_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCQ2288094.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMLCYGVVPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGIFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRSIALWHTLKIEDQL
                     QQQQQETWQRYFPQIKRTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9647..10108
                     /gene="gspM"
                     /locus_tag="OHN27_003722"
     CDS             9647..10108
                     /gene="gspM"
                     /locus_tag="OHN27_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HCQ2288095.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQVTYGIVIEDLQFTLAGNEEITLRHLSFREQQ"
     gene            10108..10785
                     /gene="gspO"
                     /locus_tag="OHN27_003723"
     CDS             10108..10785
                     /gene="gspO"
                     /locus_tag="OHN27_003723"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HCQ2288096.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFVLTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10814..11290)
                     /gene="bfr"
                     /locus_tag="OHN27_003724"
     CDS             complement(10814..11290)
                     /gene="bfr"
                     /locus_tag="OHN27_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCQ2288097.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11362..11556)
                     /gene="bfd"
                     /locus_tag="OHN27_003725"
     CDS             complement(11362..11556)
                     /gene="bfd"
                     /locus_tag="OHN27_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCQ2288098.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
BASE COUNT         3058 a         2966 c         2752 g         2838 t
ORIGIN      
        1 ataaaaataa tgtatgtatt tattaattaa atacatatta ctaatataaa taaattttgc
       61 ttgattcatg caagcggcat taatactatt tatactaacg tcaatataca acccacccaa
      121 tctattttat tagaacatac atcgtgccca cactacgttt ccccttccat ctggcgaatc
      181 ataataaaga tgctgccatc aatatattga ttatatttat aagcataggg tcgattattt
      241 ttaatgtgaa ttatttccat acaactatag ttaaaaatgg acagatcatt aatcaaccaa
      301 caaacgcatt tcagtctgat ttcagccttg ccgctttatg gcgtaacgaa aatcatgctg
      361 gggtaaaaga tgcaaatccc gtggcagtta atcaggaaac acctaagctc tctatagcgc
      421 ttaatggcat tgtactcaca tcgaatgatg agacttcttt tgtcttaatt aatgagggga
      481 gcgaacaaaa gcgatactcc ctcaacgaag cccttgagtc agcgccggga acgtttatta
      541 gaaaaataaa taaaaccagc gttgtgtttg aaactcacgg gcattacgaa aaagtaactc
      601 tccatccagg gctacctgac atcatcaagc agcctgattc agaaagtcaa aatgtactcg
      661 ctgattatat tatcgccacg cctatacgtg atggagaaca gatatacggg cttcgattga
      721 accctcgtaa gggcctcaat gcatttacca ccagcttgct acaaccaggg gatatcgccc
      781 tcaggatcaa taatctttcg ctgacccacc ctgatgaggt ttcacaggca ttaagtttac
      841 tgctaactca acaaagtgct cagtttacaa ttcgtcgcaa cggcgtaccc cgcttgataa
      901 atgtttccgt cggggaactt acaggaatga atggactgcg tcatgaaagg actcaataaa
      961 atcacctgct gcttgctggc agcactactc atgccttgtg caggacacgc tgagaacgaa
     1021 caatacggcg cgaacttcaa taacgccgat atccgccagt tcgtggaaat agtgggtcag
     1081 catcttggca aaacgatcct gatcgaccct tcggtacagg gaaccatttc cgtacgcagt
     1141 aatgatacgt ttagccaaca ggagtactac cagttctttt taagtattct tgatctttac
     1201 ggttattccg tgatcacgct ggacaatggt tttctgaaag tggttcgctc agctaatgta
     1261 aaaacatcgc cagggatgat tgctgacagt tctcgtccag gcgtaggtga tgagttggtc
     1321 acccgaattg taccgcttga gaacgttcct gctcgtgacc tggcccccct gctccgccag
     1381 atgatggatg cgggtagcgt cggtaatgtt gtgcattatg aaccctccaa cgttcttatt
     1441 ctgaccggtc gtgcctccac cattaataaa ctgattgaag tcataaagcg cgttgatgtc
     1501 atcggcacag agaagcagca aattattcat ctggaatatg cgtcagcgga agatctcgcc
     1561 gagattctta atcaattaat cagcgaaagc cacggtaaaa gccagatgcc agccctcctc
     1621 tccgcgaaga ttgtggcgga taagcgaacc aactctctta tcatcagtgg accggaaaaa
     1681 gcacgccagc gcatcacttc attactgaaa agccttgatg tcgaagagag cgaggaagga
     1741 aatacccggg tttattacct gaaatatgct aaagccacga atctggtgga agtgctaacc
     1801 ggtgtttccg aaaagctgaa agatgaaaaa gggaatgcgc gtaagccctc ctcttctggc
     1861 gcgatggata acgtcgccat taccgccgat gaacagacta actctctggt cattaccgct
     1921 gaccagtccg tccaggaaaa actcgccacg gtaattgcgc gtctggacat tcgccgtgca
     1981 caggtgctgg ttgaggcaat catcgttgaa gttcaggatg gaaatggact aaacctcggc
     2041 gtgcaatggg cgaataaaaa cgttggcgca cagcaattta ccaataccgg attaccgatt
     2101 tttaacgctg cgcaaggtgt ggctgattat aaaaagaatg gtgggatcac cagcgcgaat
     2161 cctgcctggg atatgtttag cgcctacaat ggcatggccg caggcttctt caatggcgac
     2221 tggggagtac tgcttaccgc gctggccagt aacaataaaa atgacatcct cgccacccca
     2281 agcatcgtaa cgctggataa taaactcgcg tccttcaacg tggggcagga tgtgccggtg
     2341 ctatccgggt cacagaccac ttcaggggat aacgtcttta ataccgtcga acgcaaaacg
     2401 gtggggacaa aactcaaagt tactccgcag gtcaatgaag gcgacgcggt gttgctcgaa
     2461 atagagcagg aagtctccag cgttgactct tcctctaact cgacgctcgg cccgacgttt
     2521 aatacccgta ctattcaaaa cgccgtgctg gtcaaaaccg gtgaaacggt ggtcctgggc
     2581 ggattgctgg atgatttttc taaagagcaa gtgtcaaagg ttcctctgct tggcgatatt
     2641 cctttagtgg ggcaactctt ccgctatacc tccaccgagc gcgctaaacg caacctgatg
     2701 gtatttatcc gtccgacgat tatccgtgac gatgatgttt atcgctcact gtcaaaagag
     2761 aaatacaccc gttaccgtca ggagcaacaa cagcggatcg acgggaaatc aaaagcgctg
     2821 gttggctcgg aagatttgcc ggtgctggat gaaaacacgt tcaacagtca cgcccctgcg
     2881 ccatcgtcac ggtgaggcat tcatatgaga attcactcac cgtaccccgc cagttgggcg
     2941 ctggcacaac gaattggtta tctctattca gagggcgaga ttatttatct cgccgatacg
     3001 ccattcgagc ggttactcga tattcaacgt caggttggcc agtgccagac catgaccagc
     3061 ttgtcacagg ctgattttga agctcggctg gaagcggtat tccatcagaa taccggtgag
     3121 tcgcaacaga ttgcgcagga tatcgatcaa tccgtcgatc ttctctcgct ttcggaagag
     3181 atgcccgcaa atgaagatct cctgaatgaa gattcagcgg caccggttat ccgcttgatc
     3241 aatgcgattt tgagtgaggc catcaaagaa accgcctctg atatccacat tgaaacctat
     3301 gaaaaaacaa tgtcgatccg ttttcgcatc gacggcgttt tgcggacaat tttacagcca
     3361 aacaaaaaac tggcggcact gcttatctcc cgaattaagg tcatggctcg tcttgatatc
     3421 gccgaaaaac gtattccaca ggatggaaga attagtttgc gtatcgggcg acgtaacata
     3481 gatgtccgcg tatccacact gccgtccatc tatggtgaac gcgccgtact ccgcctgctg
     3541 gataaaaaca gcctccagct ttcattgaac aacctgggga tgacggcagc ggataagcag
     3601 gatttagaaa atctcattca gcttccgcac ggtattatcc tggtgacagg gccgacaggc
     3661 tccggtaaaa gcaccacgct ctacgccatc ctttcggcgc tgaatactcc cggccgcaat
     3721 attctgacgg tagaagatcc cgtggaatat gagctggaag gcattgggca aacgcaggtg
     3781 aatacccgtg tggatatgtc tttcgctcgc ggcctgcgcg ccatacttcg ccaggacccg
     3841 gatgtcgtca tggtggggga aattcgtgat acagaaaccg cgcagattgc ggttcaggcc
     3901 tcgctcaccg gccatctggt actctcaaca ctccacacta acagtgcatc aggcgcagtg
     3961 acccggctcc gcgacatggg cgtcgaatca ttcctgcttt cgtcttccct ggcagggatt
     4021 atcgcgcaac gtctggttcg tcgcctgtgt ccgcaatgcc gacaattcac gcccgtatca
     4081 ccccaacaag cgcagatgtt taaatatcat cagctcgcgg tgacaacaat tggcactccc
     4141 gtaggctgcc ctcattgcca tcaatccggc tatcaggggc gcatggcgat ccacgaaatg
     4201 atggtggtga cgccggaatt acgggccgct attcatgaaa atgtggatga acaagcactg
     4261 gagcgactag tccggcaaca acacaaggcc ttaatcaaaa atggcctgca aaagtgataa
     4321 gcggtgacac ctcctgggat gaggttatgc gcgtcgccag tgccacgctg gagagcgaag
     4381 catgaattat cgctatcgcg ccatgaccca ggatggtcaa aaattgcaag ggatcattga
     4441 tgctaacgat gaacgtcagg cacgactgcg gctgcgtgaa gaagggcttt tcctgctgga
     4501 tattcgcccc caaaaaagtt cgggagtaaa aacacgtcgc ccgaggatca gccatagtga
     4561 actgacgctt ttcacccggc agttggcaac cttaagcgca gcggcattac ccctggaaga
     4621 gagccttgcc gtaatcggtc aacaaagcag taataaacga ctgggtgacg tgttaaatca
     4681 ggtacgcagc gccatccttg aagggcatcc cctttccgat gcattacagc attttcccac
     4741 gcttttcgat tcgctctatc gtaccctggt aaaagcgggc gaaaagagcg ggctgctggc
     4801 cccggtgttg gaaaagctgg ctgattacaa tgaaaaccgg cagaaaatcc gcagcaagct
     4861 cattcagtca ctgatctacc cctgtatgct cactacggtg gcgattgggg tcgtgattat
     4921 tctcctcact gctgtcgtgc ccaaaattac cgaacagttc gtgcatatga agcagcaact
     4981 gccgctgagt acacgcattc ttttaggtct gagcgacacg ttgcaacgta ccggcccgac
     5041 attattagcg acagtgttta ttgtcgctgt aggtttctgg ctctggttaa aacgcggcaa
     5101 taaccgccac cgttttcatg ccatgttgct gcgcgttgcg ctcatcggcc cgctgatttg
     5161 cgccattaac agcgcacgct atctccgcac tttaagtatt ttgcaatcca gcggcgtccc
     5221 tctgctggat gggatgaatt tgtccaccga aagcctcaac aacctcgaaa ttcgccagcg
     5281 tctggcaaat gcggcagaga acgttcgcca gggtaacagc attcatcttt cgctggaaca
     5341 aaccgcaatt ttcccgccga tgatgctcta catggtggcc tctggcgaaa aaagcgggca
     5401 gctcggcaca ttaatggtca gagccgcaga taaccaggag acactccaac aaaatcggat
     5461 cgccttaacg ctctccatct tcgagccagc actcattatt acgatggcac tgatcgtcct
     5521 gtttattgtc gtgtcggtac tccaacctct tcttcaactt aactcaatga ttaattaagg
     5581 aaagcatatg cgcgcaacgg ataagcaacg cggttttaca ttactggaaa ttatggtggt
     5641 gatcgtcatt atcggcgtac ttgccagcct ggtggttcct aacctaatgg gcaataaaga
     5701 aaaggcggat aagcaaaaag ccgtcagcga tattgtggcg ctggaaaacg cccttgatat
     5761 gtacaaactc gacaatcatc actatccaac cacaaatcag gggcttgaat ccttagtaga
     5821 agcgccgaca ctgccaccgc tggccgcaaa ctataacaag gaaggttata tcaagcgtct
     5881 gcctgccgat ccctggggca atgattatgt cctcgttaat cctggtgaac atggtgcata
     5941 cgatctgctt tcagcagggc ccgatggtga aatgggaacc gaggacgaca tcaccaactg
     6001 gggtttgagc aagaagaaaa agtaagtcag cgatgaatca gcaacgcggg tttaccctgc
     6061 tggagatgat gctggtgctg gcgctggtcg ctatcacggc aagcgtggtg ctcttcacat
     6121 atggccggga ggatgtcgcc aacactcgtg cgcgcgaaac agcggcgcgg tttaccgctg
     6181 cacttgaatt agccatcgac cgcgccacgc tcagtggtca gcctgtcggt atccatttct
     6241 ctgattcagc atggcgcatt atggtaccgg gcaaaacacc atcagcctgg cgctgggttc
     6301 cattacaaga agatgccgca gacgagagcc aaaatgactg ggatgaagaa ctctcaattc
     6361 atcttcagcc atttaaacct gatgacagca accagccaca ggttgtgatt ctggccgacg
     6421 gacaaatcac gcccttctct ttgttgatgg ctaacgccgg gaccggtgag ccgctcctga
     6481 cgttagtctg ttcaggctca tggcccctgg atcaaacgct cgccagagat acccgcccat
     6541 gaacaaacaa tcagggatga cactgcttga agtcttactg gcgatgagta ttttcaccgc
     6601 tgttgctctg actttaatga gcagtatgca agggcaacga aatgccatcg agcgaatgcg
     6661 taacgagacg ctggcattgt ggatagcgga taaccagctt cagtcacaag attcattcgg
     6721 cgaggaaaac acgtccagtt ctggcaaaga gttaataaat ggcgaagagt ggaactggcg
     6781 cagcgatatc cactcaagca aagacggcac gcttctggaa cgcaccataa cggtgacatt
     6841 acccagcggt cagacaacct cgcttacgcg ttatcaaagc atcgataata aatccgggca
     6901 ggcacaagat gattaatcgc cagcagggtt tcacattgct ggaggtcatg gcggcgctgg
     6961 cgattttctc aatgcttagc gtgctggcgt ttatgatttt ttcgcaagct tccgagttgc
     7021 atcaacgctc acagaaagaa atccagcagt tcaatcagct acagcgcacc atcaccattc
     7081 ttgataacga tctcctgcaa ctggtcgccc ggaggaatcg gagtacggac aaaatcatgg
     7141 tgttgggtga agaagctatt tttaccacgc aaagtcgcga tccgctggct cctctcagtg
     7201 aagcacaaac cttactcact gttcactggt atctgcgaaa tcatacgctc taccgtgctg
     7261 ttcgtacttc tgtggatggc aggaaggatc aacccgccca ggcgatgctg gaacatgtcg
     7321 agagctttct tctggaaagt aacagcgggg aaagccagga acttcctctc tcagtcacgt
     7381 tacatctgca gactcagcaa tatggggggt tacagcgccg ttttgcatta cctgagcagt
     7441 tagcgagaga agagtcgcca gctcagacgc aggccgggaa taataaccat gaataatgaa
     7501 caacgcggcg tcgcgctgct cattgtactc atgttactgg cgctcatgac tgctctggca
     7561 gcagacatga cgctcagttt tcacagccag cttcagcgaa cccgccaggt aaaccatcat
     7621 ctgcaacggc aatatgatat tgaactggcg aagaaacttg cgcttgccag cctcacccag
     7681 gacgttaaag ataacgacag acagaccacg ttgcagcagt actgggcaca gccacaacag
     7741 ttgcagctgg aagatggcaa tacagtgaag tggcagttac gcgatgccca gcactgtttt
     7801 aatctcaatg cgttagcaaa aatctctgac gacccgctgg cttcccctga tttccccgcc
     7861 caggtattca gtgcgctgct gattaacgcc ggtatcgatc gcggaaacac cgatgagatc
     7921 gtgcaatcaa tcgctgacta tattgatgtc gatgactcac cacgctttca cggcgcagaa
     7981 gatagctttt accaaagcca gacgccgccc cggcatagtg ccaatcagat gctttttctg
     8041 actggcgaat tacgtcagat aaaaggcata accgaaaaca tctatcagcg gcttatcccg
     8101 tatgtctgcg ttctcccgac cacggaactt agcatcaatc ttaatatgct aacggagaac
     8161 gatattccgc tcttcagagc gttattcctc aataacatca cggatgctga cgctcgggtc
     8221 ttattgcaaa aaaggccgag ggaaggctgg ctaaccacgg atgcatttct ttactgggcg
     8281 cagcaggatt tctccggtgt gaagccactg gttgctcagg tgaaaaggca tctctttccc
     8341 tatagccgct attttacgct gagtacggaa agtatcagcg acgaacaatc ccagggatgg
     8401 caaagccata tttttttcaa ccgtaaacag cagagcgccc aaatctatcg gcgtacgctg
     8461 caactctact aatcataagg atcgtgatgc ctgaatcgtt gatggtcata cgttcttcct
     8521 cgacgctgcg taagcattgg gaatggatga cgttctccgc agatagcgta tcttcggtac
     8581 atacgctcac ggacgatctt ccgctggagt cgctggccga tcagcccggt gccggaaatg
     8641 ttcatctttt gatcccacca gagggccttt tatatcgttc cctcacgctg cccaacgcca
     8701 aatacaaact caccgcacaa acattacaat ggctggcaga ggagacgtta cccgacaaca
     8761 cccaggactg gcactggacg gttgtcgata aacaaaacga aagtgtcgag gtgataggca
     8821 ttcagtcgga gaaactcagt cgctatcttg agcgtttaca tactgccgga ctcaacgtga
     8881 cgcgggtttt gccagatggc tgctatcttc cctgggaagt agatagctgg actctggtta
     8941 accagcaaac aagctggctc attcgcagtg ccgcacatgc cttcaacgaa ctggatgaac
     9001 actggttaca gcatcttgct gcccagtttc cgcctgagaa tatgctttgt tatggtgtag
     9061 tacctcacgg cgttgcagcg gccaatccac ttatccagca tcctgaaata ccatcactga
     9121 gtttgtattc tgccgatatt gcattccagc gctatgacat gctgcatggt atttttcgta
     9181 aacagaaaac tgtaagcaaa tcgggaaaat ggctcgcccg cctggcagta agctgtctcg
     9241 ttctcgccat tctttctttt gttggcagcc ggagcatcgc gctctggcat acgcttaaga
     9301 ttgaagatca acttcagcaa caacagcaag aaacctggca acgttatttc ccgcagatca
     9361 aacgcaccca caattttcgt ttttacttta agcagcaact cgctcagcaa tatcctgaag
     9421 ctgtgccgtt gctctatcat ttacaaacac ttctgctgga acaccctgaa ctgcagttaa
     9481 tggaggcgaa ctatagccag aagcaaaagt cgctgacctt aaaaatgagt gctaagagtg
     9541 aagccaatat cgatcgtttc tgtgagttaa cacagtcctg gcttccgatg gaaaaaacag
     9601 aaaaagatcc ggtcagtggt gtatggacag taaggaactc agggaaatga ttaaatcatg
     9661 gtgggcagaa aaatccacat cggaaaaaca gatcgttgcg gcactggcgg ttctctcgct
     9721 cggcgttttt tgttggcttg gggtgataaa accgatagat acctacattg cagagcatca
     9781 gtcccacgcg caaaaaatta agaaggacat caaatggatg caggatcaag cgagcaccca
     9841 tggtttactc ggtcatcctg cgcttacgca gcccatcaaa aatatccttc tcgaggaagc
     9901 aaaacgtgag aatttggcta tcacgctgga aaacggcccg gacaatactc tgacaattca
     9961 tcctgtcaca gctcccctcg aaaacgtctc ccgctggctc actacggcac aggtaacata
    10021 cggtattgtt attgaagacc ttcagtttac tcttgccgga aacgaagaga tcaccctcag
    10081 gcatttgtcc ttcagggagc aacaataatg acaatgctac tgccgctctt cattctggtt
    10141 ggttttattg caggttattt tgttaatgcc atcgcctatc acctctcgcc gttggaggat
    10201 aaaacggcgt taacgtttcg ccaggtattg gttcatttca ggcaaaaaaa atatgcctgg
    10261 catgatacag tgcccctgat actttgtgtt gctgctgcta tcgcctgcgc cctggcacct
    10321 ttcacgccca tcgtgactgg cgcactcttt ctctatttct gtttcgtact cacgctcagt
    10381 gttattgatt ttcgcactca gctcctgccc gacaaactca ccttaccgct gctctggctt
    10441 ggcttggtat ttaatgcgca gtatggatta attgatttac atgatgcggt ttacggcgcg
    10501 gtagcgggct atggggtgct gtggtgtgtt tactggggcg tctggttagt ttgtcacaaa
    10561 gagggattgg gctacggtga tttcaagcta ctggccgctg caggcgcatg gtgtggctgg
    10621 caaacgttgc caatgatact gctgattgcc tcgctgggtg gcattggtta cgccatcgtt
    10681 tcacaacttc tgcaacgccg gactataacc actatcgcat tcggcccgtg gcttgcgctc
    10741 ggcagcatga taaacctggg gtatctggcc tggatctctt attaaccggg agggttcccc
    10801 ctcccgacac ggctcaacct tcttcgcgga tctgtgcttg cagataattt tgcaggccca
    10861 tcttctgaat cagatcaagt tccgtttcca gccagtcgat atggccttct tcatcacgca
    10921 aaatttctat catcatatcg cggctgacgt aatcatgaac gctatcggca taaccaattg
    10981 cctcacgcaa attcttcgcg ccatccagct caagtgccag atcagaacgc agcatttcct
    11041 caacatcttc accaatgttc agtttgccca ggtcctgtaa gtttggaaga ccttccagaa
    11101 aaagaatgcg ctcaatataa cgatcggcgt gtttcatctc atcaatggat tcatgatact
    11161 ccacatcatt gagacgtttg agaccccagt ttttaaacat tcgggcatgg agaaagtact
    11221 gattgattgc gacaagctca tttcccaaca gtttgttgag ataatttata actttagtat
    11281 cacctttcat tttttgactc cttcgcttcc actctttgaa gagtagaacg gcttacgagt
    11341 aagtcaaaaa acacactccg tttatgcgga ctccttaaac tccggcagct gcattaattc
    11401 atcctccatc acttcacgcg cggcccggac acatttaccg cactgattgc cgaccggaat
    11461 aaattttttt aattgctgga acgagtgagg ggaaaactgg cgcacagcct gacgaatttt
    11521 tttatcgctg ataccattac aaagacaaac gtacatagag actcccgtat actttcttca
    11581 aaaataaatg aaaatagttc ttatttcaat tacg
//
LOCUS       DAJOSY010000125        11581 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJOSY010000125 DAJOSY010000000
VERSION     DAJOSY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.125"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            217..5637
                     /locus_tag="OHN27_003726"
     CDS             217..5637
                     /locus_tag="OHN27_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HCQ2288099.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLVTAENTIIGGNATGI
                     GTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSSEDKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNQGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYKLTNALKGDGLMRVQLSSSDKIFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVNVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDMNVMPGG
                     ALRVAKTTISGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5860..6126
                     /gene="hipB"
                     /locus_tag="OHN27_003727"
     CDS             5860..6126
                     /gene="hipB"
                     /locus_tag="OHN27_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCQ2288100.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPESTEQQNLEW"
     gene            6126..7448
                     /gene="hipA"
                     /locus_tag="OHN27_003728"
     CDS             6126..7448
                     /gene="hipA"
                     /locus_tag="OHN27_003728"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HCQ2288101.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK"
     gene            7760..7968
                     /locus_tag="OHN27_003729"
                     /pseudo
     CDS             7760..7968
                     /locus_tag="OHN27_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8223..9449
                     /locus_tag="OHN27_003730"
                     /pseudo
     CDS             8223..9449
                     /locus_tag="OHN27_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            9463..9993
                     /locus_tag="OHN27_003731"
     CDS             9463..9993
                     /locus_tag="OHN27_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2288102.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            10006..10509
                     /locus_tag="OHN27_003732"
     CDS             10006..10509
                     /locus_tag="OHN27_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2288103.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRNDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            10568..11482
                     /locus_tag="OHN27_003733"
     CDS             10568..11482
                     /locus_tag="OHN27_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2288104.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPRSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         3231 a         2472 c         2989 g         2889 t
ORIGIN      
        1 taagcaacaa taactcatag ttcatattta aataaaataa gaactatgca atttttaatc
       61 tatcaattcg aaagcattag caaattagcc aaattgacac cttcccacct ccaactaaca
      121 ctcaatacag acctctgaca atttaataag tttcattttt aatgtctgac gactcatctc
      181 tttcgtggct gtattgtttg tatatggagc gtttatatga atagaatcta tcgcgtgata
      241 tggaattgca ctctacaggt atttcaggcc tgctcggaat taactcgcag ggcaggtaaa
      301 acatcgacgg ttaatttgcg taaatcctct ggactgacaa cgaaattcag tagattgacg
      361 ctgggtgttt tgctggcact aagcggttca gcgtctggtg caagtctgga agttgataat
      421 gatcagatta ccaatattga tactgatgtt gcttatgatg cctacctggt tggctggtat
      481 ggcactggag tgcttaatat tttggctggc ggtaatgcct ccttaaccac tattactacc
      541 agcgtcattg gcgctaatga ggactcagag ggcaccgtta atgttttggg tggcacctgg
      601 cgattgtatg atagcggaaa taatgcaagg cctttaaatg tgggtcaatc cggaacgggg
      661 acgctgaata ttaaacagaa gggtcacgtc gatggaggct atttaagatt aggttcttcg
      721 acaggaggcg tcgggacggt caatgttgag ggagaggact ctgttctgac gaccgaatta
      781 ttcgaaatag gtagctatgg cacaggttca ttaaatatta cggataaggg ttacgtcacg
      841 agttcaatcg tcgccatttt aggctatcag gcgggcagta atggccaggt tgtcgttgaa
      901 aagggtggcg agtggctaat aaaaaataat gattcctcaa ttgaatttca aattggtaat
      961 caaggaactg gggaggcgac tattcgcgag ggggggctgg ttacggctga aaatacgatt
     1021 atcggtggca atgccaccgg tatcggaacc ctgaatgtgc aggatcaaga ctctgtcatc
     1081 acggtacgca gactctataa tggatatttc ggtaatggca cagtcaatat ttccaataat
     1141 ggactgatta ataacaaaga atattcattg gtgggcgttc aggacggttc ccacggtgtc
     1201 gtcaacgtga ccgataaagg gcattggaat ttcctcggaa cgggcgaagc tttccgctat
     1261 atctatatcg gtgatgctgg cgacggtgaa cttaatgtct cgagtgaaga caaagtagat
     1321 tcgggaatta tcactgcggg gatgaaagaa acaggcacag gcaacattac tgttaaggat
     1381 aagaactccg ttatcactaa tctcggaact aatcttggtt atgacggcca cggcgaaatg
     1441 aatatcagta atcaggggct tgttgtcagc aacggaggaa gttcactcgg ttatggagaa
     1501 accggcgtcg ggaatgtcag catcaccacg gggggaatgt gggaggtcaa taagaatgtc
     1561 tataccacca ttggtgttgc gggcgtcgga aacctcaata ttagcgatgg cggtaagttc
     1621 gtatcgcaaa atattacttt tttgggcgat aaagcaagcg gtatcggcac attgaacctg
     1681 atggatgcga catcatcgtt cgatactgtg ggtatcaatg tcggtaattt tggtagcggc
     1741 atcgtaaatg tcagtaatgg cgccaccctt aattcaacgg gctatggatt tatcggagga
     1801 aatgcctccg gtaagggaat agttaatatt tcaacggata gtctctggaa tttaaagacg
     1861 tcttctacta acgcccaatt gctacaagtc ggtgtattag gcacgggtga actgaatatt
     1921 accaccggag gtatagttaa agcgcgtgat acacagatag ctctcaatga caaaagtaag
     1981 ggcgacgtga gggtggatgg gcagaactct cttcttgaaa cattcaatat gtacgtaggg
     2041 acatctggta cgggtacgtt aaccctgacg aatagcggca cgctgaatgt cgaaggtgga
     2101 gaagtttact taggtgtttt tgaacctgct gtaggaacgc taaacattgg tgcagctcac
     2161 ggtgaggcgg cggcagatgc cgggtatata accaacgcga cgaaagtaga gtttggttct
     2221 ggcgaaggtg tttttgtctt taatcatact aataacagtg atgccggcta ccaggtcgat
     2281 atgctgatta cgggtgacga taaagacgga aaagtgatcc atgatgcagg ccatacggtg
     2341 ttcaatgcag ggaatactta tagcggtaaa acgctggtca atgacggcct cctgaccatt
     2401 gcgtctcata cggcagatgg ggtaacgggc atgggttcga gtgaagtcac cattgcaagc
     2461 cccggtacgc tcgacattct cgcatcaacg aacagtgcag gagattacaa gctgaccaat
     2521 gcgctcaaag gcgatggttt gatgcgagtg cagctgtcat cctccgacaa gatatttggc
     2581 tttacccatg caacagggac tgaattcgcc ggtgttgccc aactgaaaga cagtaccttc
     2641 actctggaac gcgacaacac cgctgcgctt actcacgcga tgttgcagtc tgacagtgaa
     2701 aataccacat cggtaaacgt gggagagcaa tccattggtg gactggccat gaatggcggt
     2761 acgctcattt tcgatacgga tattcctgct gcgacgcttg cagagggata tatcagcgtc
     2821 gatacgctgg ttgtcggcgc gggtgactac acctggaaag gccgtaacta tcaggtaaac
     2881 gggacgggcg acgtgcttat cgacgtgcct aaaccgtgga atgatcccat ggcgaataac
     2941 cctctgacga cgctcaattt gctggaacac gatgatagcc atgtcggcgt tcaactggtg
     3001 aaggcgcaaa cggttattgg gtcgggtggc tcattaacgt tacgtgattt acagggcgac
     3061 gaggtggaag cggacaaaac gttacacatt gcgcaaaacg gaacggtggt cgccgagggt
     3121 gattatggat tccgcctcac gaccgcacca ggtgatggtt tgtacgttaa ctatgggctg
     3181 aaagcgctga acatccatgg tgggcaaaag ctgacgttag ccgaacatgg cggagcctat
     3241 ggcgcaacgg ccgatatgtc ggcaaaaatc ggtggtgaag gggatctggc aatcaatacg
     3301 gtgcgacagg tttcgctttc caacggtcag aacgactatc agggggcaac ctacgttcag
     3361 atggggacat tacgtaccga tgcggatggt gcacttggca acacccggga actgaacatc
     3421 agcaacgcgg ccatcgtcga tcttaatgga tcgacgcaga cggtagagac attcaccggg
     3481 cagatgggtt cgactgtttt gttcaaagag ggggcgctga cggtaaataa aggtgggatc
     3541 agtcagggtg aactgacagg tggcggaaac ctgaatgtta cagggggaac gctggctatc
     3601 gaggggctta atgcacgcta caatgcgtta accagcatta gcccaaatgc ggaagtcagc
     3661 ctcgataata cgcaggggtt aggcagagga aatattgcca atgacggtct gttaacgcta
     3721 aaaaacgtga ctggcgaact gcgcaatagc ataagcggga agggtatcgt gagcgcaacc
     3781 gccaggacag atgtagagct ggatggcgat aatagccgct ttgtggggca attcaacatt
     3841 gatacaggca gcgcgctcag cgtcaacgag cagaaaaacc tgggtgatgc ttccgttatc
     3901 aataatggcc tgctcaccat ctccactgag cgtagctggg cgatgacgca cagtatcagc
     3961 ggtagcggtg atgtgacaaa actgggtacc gggatcctga ctcttaacaa cgattccgcg
     4021 gcgtatcagg gtacgacgga tatcgtgggg ggggaaattg ctttcggttc cgactctgcc
     4081 attaatatgg caagtcaaca cattaatatc cataacagcg gtgtgatgtc gggaaatgtc
     4141 accactgcag gtgatatgaa cgttatgcct gggggggcac tgcgtgtcgc taaaaccact
     4201 atcagcggca acctggagaa tggcggcacg gttcaaatga atagcgaagg ggggaaaccg
     4261 gggaatgtac tgaccgttaa cggcaactat accggaaaca atggcctgat gacgttcaac
     4321 gcgacgctgg gcggcgataa ttcgcccacc gataagatga acgtgaaagg cgatacccaa
     4381 gggaacactc gcgttcgggt tgataacatt ggcggcgtcg gtgcacaaac ggtcaacggt
     4441 attgaactca ttgaggttgg cggtaattct gcaggtaact tcgcgctgac caccggaact
     4501 gtcgaagctg gggcttacgt ctacacgctg gctaaaggga aggggaatga cgagaaaaac
     4561 tggtatctga ccagtaaatg ggacggcgta acgccagcgg atacacccga tcccatcaat
     4621 aatccccctg ttgtggatcc ggaaggccca tcagtttatc gcccggaggc cggaagctat
     4681 atcagcaaca ttgccgcagc caactcgctg tttagccatc gtttacacga ccgtctgggt
     4741 gagccgcagt atacagattc actgcattct caggggtcgg caagcagtat gtggatgcgt
     4801 catgtcggag ggcacgaacg ttcaagggcc ggtgacggtc agctaaatac tcaggctaac
     4861 cgctatgtat tgcagctagg cggcgatttg gcgcagtgga gtagcaacgc gcaggatcgc
     4921 tggcatcttg gcgtgatggc aggctacgcc aatcagcaca gtaatactca gagtaatcgt
     4981 gtgggttata aatcggatgg gcgcatcagc ggttacagcg ctgggctgta cgcgacctgg
     5041 tatcagaacg atgcgaataa gaccggcgct tatgttgaca gctgggcgct gtataactgg
     5101 tttgataaca gcgtcagttc cgataaccgt tctgctgacg actatgattc tcgcggtgtg
     5161 acggcctctg ttgagggtgg gtataccttt gaagcgggaa catttagcgg cagcgaaggg
     5221 acgctgaata cctggtacgt ccagccacag gcgcaaatca cctggatggg tgtgaaagat
     5281 tccgaccata cccggaaaga cggaacgcgc attgaaacgg aaggcgacgg aaatgtgcaa
     5341 acgcgacttg gggtgaaaac ctacctgaat agccatcacc agcgtgacga tggtaaacag
     5401 cgtgagttcc agccttacat tgaagcgaac tggatcaaca atagcaaagt ctacgccgtg
     5461 aagatgaatg gtcaaaccgt aggccgtgaa ggtgcgcgta atctcggtga agtacgtacc
     5521 ggggttgagg cgaaagtaaa taacaacctt agcctgtggg ggaatgtcgg tgtgcaacta
     5581 ggtgataaag gctatagcga tactcagggc atgctgggag tgaaatatag ctggtaaacc
     5641 gtataagccg catgtcgaga tggcatgcgg cttaatattg ccgacttcaa acggcgcatc
     5701 aacgccttat ttaaatcctc ctttttatcc gcgatcgcgg atatcgcagc gtttatcccg
     5761 tagagcggat aagatgtgtt tccagattga cttatcctca ctaaaggata aaacttataa
     5821 tatcccctta agcggataaa cttgctgtgg acgtatgaca tgatgagctt tcagaagatc
     5881 tatagcccaa cgcaattggc gaatgcaatg aaactggttc gccagcaaaa tggctggacg
     5941 cagagcgagc tggcgaaaaa aattggtatt aagcaggcga cgatttccaa tttcgaaaac
     6001 aaccctgaca ataccacgct cacgacattt tttaagattt tacagtcgct tgaactctca
     6061 atgacgctat gcgacgcgaa aaatgcctcg ccagaatcaa cagaacagca aaatctggag
     6121 tggtaatgcc taaacttgtc acttggatga acaaccagcg ggtaggcgag ttaacgaagt
     6181 tagccaacgg cgcgcacacc tttaagtatg caccggagtg gttagcaagc cgttatgcca
     6241 gaccgttgtc actttcgctg ccattgcaga gggggaatat cacctctgat gccgtattta
     6301 acttcttcga taacctgtta cccgatagcc cgattgtacg tgaccggatc gttaaacgtt
     6361 atcatgccaa atccagacaa ccgtttgatt tattgtcaga aatagggcga gacagcgttg
     6421 gtgccgtgac gttaataccc gaagacgaaa ccgtaacgca tccgataatg gcatgggaaa
     6481 agcttactga agccagactt gaagaagtat taacggctta taaagcagat atcccgctag
     6541 gcatgattag agaagaaaat gactttcgca tctcggttgc tggcgcacag gagaagacag
     6601 cactgctcag aataggcaat gactggtgca ttccgaaagg aataacgccg acgacgcaca
     6661 tcattaaatt accgattggc gaaatcaggc agcccaatgc gacgctcgat ctcagccaaa
     6721 gcgttgataa tgagtattac tgtctgctgc tggcgaaaga acttgggttg aatgttccgg
     6781 acgcagaaat cattaaagcg ggaaatgtgc gcgcgttagc ggtcgaacgt tttgacaggc
     6841 gttggaatgc tgagcgaacg gttttacttc gcttgccaca ggaggatatg tgtcagacat
     6901 tcggtttacc ttcatcggtg aaatatgaat cagatggagg cccaggcatc gcgcggatca
     6961 tggctttttt gatggggtcc agcgaggcgc tgaaagatcg ctatgatttt atgaaattcc
     7021 aggtcttcca gtggttgatt ggcgcaacgg acggtcatgc aaaaaacttc tccgtattta
     7081 ttcaggctgg cggcagttat cgactcacgc cattttacga catcatttca gcatttccgg
     7141 tccttggcgg tacgggaata cacatcagcg atctcaaact ggcaatgggg cttaacgcat
     7201 ccaaaggcaa aaaaacggca atcgataaaa tttatccgcg acattttttg gcgacagcaa
     7261 aggtgctgag attcccggaa gtgcagatgc atgaaatcct gagtgacttt gccagaatga
     7321 ttccagcagc actggataac gtgaagactt cattaccgac agattttccg gagaacgtgg
     7381 tgacggcagt tgaaagcaat gtgttgaggt tgcatggacg gttaagccga gaatacggta
     7441 gtaagtgaga tatggggttt ggtcattgtt aatgagcatg acaatcatga ccgccaacga
     7501 ctggattctt ccttggttta tattggtgcg cataatgtat attatgttaa atcatgtctg
     7561 agtgatgcga agtgcttttc tggtagtcgt tattcgttca gggttcaaga acttaagcct
     7621 ctcctctttc ataattggcc tgtgcatgga gaaatagcct ggttattccc tgccccatgc
     7681 taagagcgca gtgtctgtcg catagacact gcatacgaaa acaccagagg gtgctgttac
     7741 ttatttaagg acggatcaag cgggaatgag tccactccgg tatcagaaat ggctacgtct
     7801 caatgaagtc aggcgaccga tgctgaatga acattacgat gtcaccacag cagcctatgc
     7861 tgtcggttac gaaagctatc ccatttcagt cgggaatatt cgcggatgtt tggagagtca
     7921 cccaagagag atattaccag gttaagaaag tctgtcggac agctttaata aaccctgcac
     7981 ttatctgttt agagcaattc ggtgttagtt tcagcaagca aacattaacc atagctaatg
     8041 atttatagcc atattaacca ttggtagctc ttgacctttc ctgacacttc ccttgttcgc
     8101 aataatattc tgtttagtca cggtttataa ataacatgca taatgaaatc gtcatgttat
     8161 ttagcgacat aaaatgtttt tgcaaatact ctcttcaaca agtgcagcaa taaacttgtt
     8221 gtggaaccaa tatccaggtc gcaggatatc gctattctac gcaaggtttt tataacttat
     8281 ccgacagtgc ctacagtcga atgagtggtt acaccgtcaa gcctcctacc ggagacacca
     8341 atgagcagac acaatttatt gattatttta atctgttcta cagtaagcgt ggtcaggaac
     8401 aaataagcat ctctcagcag cttggaaatt acggtacgac atttttcagt gccagtcgcc
     8461 aaagttactg gaacacgtca cgcagcgacc agcaaatatc atttggatta aatgtgccgt
     8521 ttggtgatat tacgacttcg ctgaattaca gctattccaa taatatatgg caaaacgatc
     8581 gggatcattt actcgctttt acgcttaatg ttcccttcag tcattggatg cgtacagaca
     8641 gtcagtcggc atttcgtaat tcaaacgcca gttacagtat gtcaaacgat ttgaaaggcg
     8701 gcatgaccaa tctatcgggg gtttatggca ctctgctgcc ggataataac ctgaattata
     8761 gcgttcaggt cggtaacacc cacggaggta atacatcgtc tggcaccagt ggttacagtt
     8821 ctcttaatta tcgtggagct tatggtaata ctaatgtcgg ttacagtcgg agtggtgaca
     8881 gcagccagat ttattacgga atgagtggtg ggattattgc tcatgctgat ggcatcacct
     8941 ttggacagcc gctgggcgac acaatggttc tggttaaggc tcctggtgct gataatgtca
     9001 aaatagagaa ccagaccgga attcataccg actggcgtgg ctatgccata ttaccatttg
     9061 cgacagaata tagagaaaac cgtgttgctc ttaacgcgaa ttcccttgca gataatgttg
     9121 aactggatga aaccgtggtc actgtcatcc caactcacgg tgctattgcc agagcaacat
     9181 ttaatgcaca aatcggcggg aaagtattaa tgacgttgaa gtacggtaat aagagcgttc
     9241 cattcggtgc aattgtcaca cacggagaga ataaaaatgg cagcattgtc gcggaaaatg
     9301 gtcaggttta tctgactgga cttccacagt cagggcaatt acaggtttca tggggcaaag
     9361 ataaaaactc aaactgtatt gtcgagtaca agcttcctga agtttctcct ggtaccttac
     9421 tgaaccagca gacagcaatc tgtcgctaag gaacaataga ttatgaaata caataacatt
     9481 attttcctcg gtttatgtct ggggttaacc acctattctg ctttatccgc agatagcgtt
     9541 attaaaatta gcgggcgcgt cctcgattat ggctgcacag tctcatcgga ttcgcttaat
     9601 tttaccgtag atctccaaaa aaacagtgcc agacaatttc caacgaccgg tagcacaagt
     9661 ccagccgtcc cttttcagat tacgttaagt gaatgcagca aagggacaac gggggttcgg
     9721 gttgcattta acggtattga ggatgcagaa aataatactt tgttgaaact ggatgaagga
     9781 agcaatacgg cctccggttt gggtatagaa atattggacg caaatatgcg tccggtgaaa
     9841 ctgaatgatc ttcatgccgg gatgcagtgg atcccactgg taccagaaca gaacaatatt
     9901 ttgccttact ccgctcgtct gaagtcaact cagaagtccg tcaatccggg actggtgagg
     9961 gcttcggcaa cctttaccct tgaatttcaa taatcagagt cagatatgaa aagacttcac
    10021 aagaggttcc tgttagctac gttttgcgcg ttattcacag caactctcca ggccgccgat
    10081 gtcactatca ctgttaatgg tcgggtagtc gctaaaccct gcactattca aaccaaagaa
    10141 gctaacgtta atctcgggga tctttatacg cgcaatctgc aacaacctgg ttctgcatct
    10201 ggctggcaca atattacttt gtcattaacc gattgtccgg ttgaaacaag tgcagtgacg
    10261 gcaatcgtga caggttcaac tgacaatacg ggttattaca aaaatgaagg tactgccgaa
    10321 aatattcaga tagagctgag gaatgaccag gatgctgcgt taaaaaatgg cgatagcaaa
    10381 acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc aagagctatc
    10441 acggtgaatg gaaacgcaag ccagggaacg atcgaggcgc taatcaatgt gatctacacc
    10501 tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt taaccttagg
    10561 atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc ttctattaat
    10621 ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta ttggagcagg
    10681 tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga atctggttgt
    10741 agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg acactgatca
    10801 tataaaccta gtacaaggct cagctttcgc aggctcccta cagtcatata aaggatcgct
    10861 gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg tattggatat
    10921 tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag ttggggcggc
    10981 cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt ataaaattgc
    11041 aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa ataacaatgt
    11101 tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcaccgtgg atctcccgga
    11161 ctttcctaga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac aaaagctctc
    11221 attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta acacagcacc
    11281 tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa aaatactggc
    11341 tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc tgggtttaag
    11401 cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt cagtcattgg
    11461 tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct ccattcacga
    11521 aaattgcgcg gataattatt ttgtgaaggc tattagccta cacctgtaag aaaatccgcg
    11581 c
//
LOCUS       DAJOSY010000126        11490 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJOSY010000126 DAJOSY010000000
VERSION     DAJOSY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.126"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(59..1651)
                     /gene="lsrK"
                     /locus_tag="OHN27_003734"
     CDS             complement(59..1651)
                     /gene="lsrK"
                     /locus_tag="OHN27_003734"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HCQ2288105.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENATIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1730..2683)
                     /gene="lsrR"
                     /locus_tag="OHN27_003735"
     CDS             complement(1730..2683)
                     /gene="lsrR"
                     /locus_tag="OHN27_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCQ2288106.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2932..4467
                     /gene="lsrA"
                     /locus_tag="OHN27_003736"
     CDS             2932..4467
                     /gene="lsrA"
                     /locus_tag="OHN27_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCQ2288107.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGNEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4461..5489
                     /gene="lsrC"
                     /locus_tag="OHN27_003737"
     CDS             4461..5489
                     /gene="lsrC"
                     /locus_tag="OHN27_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCQ2288108.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVTTLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5489..6481
                     /gene="lsrD"
                     /locus_tag="OHN27_003738"
     CDS             5489..6481
                     /gene="lsrD"
                     /locus_tag="OHN27_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCQ2288109.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6493..7515
                     /gene="lsrB"
                     /locus_tag="OHN27_003739"
     CDS             6493..7515
                     /gene="lsrB"
                     /locus_tag="OHN27_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HCQ2288110.1"
                     /translation="MTLHRFKKIALLSVLGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPSWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7542..8417
                     /gene="lsrF"
                     /locus_tag="OHN27_003740"
     CDS             7542..8417
                     /gene="lsrF"
                     /locus_tag="OHN27_003740"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCQ2288111.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEYQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8441..8731
                     /gene="lsrG"
                     /locus_tag="OHN27_003741"
     CDS             8441..8731
                     /gene="lsrG"
                     /locus_tag="OHN27_003741"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCQ2288112.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8788..9546
                     /gene="tam"
                     /locus_tag="OHN27_003742"
     CDS             8788..9546
                     /gene="tam"
                     /locus_tag="OHN27_003742"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCQ2288113.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9550..10464)
                     /locus_tag="OHN27_003743"
     CDS             complement(9550..10464)
                     /locus_tag="OHN27_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCQ2288114.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10671..>11490)
                     /gene="uxaB"
                     /locus_tag="OHN27_003744"
     CDS             complement(10671..>11490)
                     /gene="uxaB"
                     /locus_tag="OHN27_003744"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCQ2288115.1"
                     /translation="VDRIVTGYPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSL
                     ATELRLDKYPLNVLIVDDIKPYKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEI
                     CAFVEKAIYEEIIPVLDLPRDELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRI
                     LPQLLAGQKAKGTLPARLTFALAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHR
                     DRVIGTQELVAIVLAEKDHWEQDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         2793 a         2865 c         2980 g         2852 t
ORIGIN      
        1 gaataactat actcaaaaac taacagccac ggtcatcatg atgtggctgt caatgaaact
       61 ataacccagg cgctttccat aacgacgtcg tcagtccatg atcaaccagc cccagctgat
      121 cctgataaac tgcctgccac ttatcgcgtg aatcctgata aagttcatgc ttttccgggt
      181 ctggtgtgtg cgtccgttcc cagcgaacca ggcgttctcc ggtttctgcc attgatgaaa
      241 aaattccggc accgacgcca gctgcaatgg cacatcctaa tgcagtggct tctttgacca
      301 ccggaatatt gacgggtaat cccgagacat cagcgagaat ttgactccat aatttccctt
      361 ttgaacctcc gcctgcaaag actaacgatg aaggatgaat attcgagaaa tcagcaattt
      421 gctgcaagtt acacgctgat acaatcgtcg cattttcttc cagcgcacgg aacaatgtcg
      481 ctttgttaca tttatccggg tcaatggaca agttaataaa ggaaggcgca gcgtgatacc
      541 aggttttaaa gcgcattctg tcggagaaga tcggcattac gccccacgac ccaggcggca
      601 cccgactggc catctcttcc agcagcgtat aggtgtcgat gcctaaacgt tcggcaatca
      661 gtttttcttc ggcacagaaa gcatcgcgga accagcgcat ggtgagtccg gtaaaaaagc
      721 ttatagattc agcttgtacc atgccaggaa taacatgagg attaacgcgc acgttcattt
      781 ctgggtctgt caccggcgcg gctaaattta caacttgctg ccagaatgtg ccgccaagaa
      841 ccgcggtttg tgccggacgc acaacgccta acccaaggca accaagctgc acgtcgcctc
      901 ctccaacgac caccggagtg cccgccttca gaccgcagag ttccgccgct tgtgaactta
      961 ccacgcccag caatgtgccg gtttctttga caggagaaag aatatcggca cgtaggccag
     1021 ccatatccag caatgcaggt ttccagtcac gggtggttag atcaagaagt cccgtggtgc
     1081 cagcgttaga gggatccacc gccagttcgc cgctgagcat ataggccagc cagtcgctga
     1141 tcatggtgat ggttgatgcc tgacggtaaa tatcggaacg atggtgcgcc agccaaagta
     1201 atctggggat ggcacttaaa gccagtgttt gtccggtcgc gcgataaact tcgttttcaa
     1261 aggtattgtt gtgcagttct ttaagttcgc taacttcgcg tgccgctctg gcatccacat
     1321 tggcgcaggc ccagatcggg gctccttcat tattatataa aacaatgcct tcacgcatcg
     1381 aacatgccga aacggcagcg atatactccg gggctatgcc ggcgttgtgc agcgcctggc
     1441 gcatacactc acacgccagt tgccagtttt tgttgagatc aaattccata gaaccaggaa
     1501 cgtccggtac tgccagatgc cgccactccg cctgtcccac tgctatttga ttgccttcca
     1561 ggtcgaatat cacagcccga atacttccgg tgcctgcatc cagcgccatc aggtagtact
     1621 ttgattctga aggggtaaag agtcgagcca ttagattatc ctcggcttat gcgttctgtc
     1681 tgttcctcta tacgttctcc atcattcccg gtaataaggt catgcaaatt taactacgta
     1741 aaatcgccgc tgctgtgtcc tgatcggtaa ccagtgcgtt gatataaccg cctttcattg
     1801 cagcggcaat tgcttcggct ttattttctc cccctgccac gccaacccgg acgggtatgg
     1861 tcttcagcgc gcttaaaggt aagccaatca gttcgttatg tattttgata tccgtgacaa
     1921 cgtcaccttt tgcatcaaaa aagtagccta aaatgtcgcc aaccgcccct tttcggccaa
     1981 tcattaactg ttcgccctgg ctgatataac cggagcgaat gatcgtcgcg tcgtcttgtt
     2041 gactcacggc cccaatgccg acaatcgcca catccgctgc ttgcgcggct agcagaacat
     2101 ctttgacgca attttcattt tttagcgtac gggcaatgtc agcggaggat gcccgcaacg
     2161 gagccggaat aatattcaca ctgcacgccg cgttaagctg cccgataccc gtcatataag
     2221 aaccgacgcc accggagagc gtgaccaggc gaatttgctg tgacgaaata aaaccactta
     2281 agcgttgcag cgtattcatg gttgcctcgc caaaaccaat cgccagcatc tgttgtggtt
     2341 gaagtaaact catcaacata tgcgccgcgc ctatccctag tcgcccgccg acatcagcat
     2401 ccgcaagccc agggatcacc cggacatgtt gcagcgaaaa ctgacgacgt aattgagttt
     2461 catattccag acagccttca aagcgagaat taatctgtac gcgaataatg ccggactgat
     2521 gccctttctc cagcaatcgc gatactttca aacgtgtcag gccgagacga tcgctgatct
     2581 cactctgggt cagcccgtcg tggtagtaaa accacgcgat ccgcgcgacc tgttcttctt
     2641 cacacattcc ctgttctgaa attaccgaat cgttgattgt cataattcat tcttcacttt
     2701 gaacatattt aaatctttaa tgcaattgtt cagttcttgc gcatttatat ctgtgatggc
     2761 aaccacagtt tgactctacg agtatgaaca aacgcaaccg tgaaaatcaa aatagcataa
     2821 attgtgatct attcgtcgga aatatgtgca atgtccacct aaggttatga acaaattaaa
     2881 agaagaaata catttgttca aaactcacct gcaaaactga acgggggaaa tatgcaaacg
     2941 agtgataccc gcgcgttacc gctactttgc gcccgctcgg tttataaaca gtattcaggg
     3001 gtcaatgtcc tgaaaggcat cgattttacg ttgcatcagg gggaggtcca cgccctgctc
     3061 ggcggcaatg gtgccggtaa atcgacgtta atgaagatta ttgccggtat tacccctgct
     3121 gatagcggta cgctggagat tgggggcaac aactacgcca gattaacgcc agttcatgct
     3181 catcagctgg gtatttatct cgttccccag gaaccgctgc ttttcccaag cctgtcgata
     3241 aaagaaaaca tcctgtttgg gctggcaaaa aaacagctct ccatgcagaa aatgaagaac
     3301 ttgctggcgg cgctgggctg ccagtttgat ctgcatagtc tggcaggatc gctggatgtc
     3361 gccgatcgcc aaatggtgga aatcctccgc gggctgatgc gcgactcgcg gattctgatc
     3421 ctcgatgaac ctaccgcctc gcttacccct gcggaaaccg aacgcttgtt tactcgcttg
     3481 caagagctgc ttgctactgg cgtgggtatt gtttttatct cgcataagct gccggaaatt
     3541 cgccagattg ccgatcgaat tagcgtgatg cgcgacggaa ccatcgcctt aagcggcaaa
     3601 accagcgaac tgtctaccga cgacattatt caggccatca cgccagtggt acgggaaaaa
     3661 tcgctctctg ccagccaaaa attatggctg gagttacctg gtaaccgccc acaacatgcc
     3721 gccggaacgc cggtgctgac actggaaaat ctgactggcg aaggcttcag gaatgtcagc
     3781 ctgacgctca atgccggaga aattctgggc ctggctgggc tggtgggagc cggacgcaca
     3841 gaactggccg agacgctcta tggtctgcgt actttgcgtg gcggacgcat tatgctgaat
     3901 ggtaacgaga tcaataaatt atccactgga gaacgtttac tgcgcggtct ggtttatctg
     3961 ccggaagatc gccagtcatc cggactgaat ctcgatgctt cgctggcctg gaatgtctgc
     4021 gcccttactc ataaccttcg tggattctgg gcgaaaaccg cgaaagataa tgccaccctg
     4081 gaacgatatc gtcgggcgct gaatattaaa tttaaccaac cggaacaagc tgcacggaca
     4141 ttatccggtg gcaaccagca aaaaatcctc attgccaaat gcctggaagc ctcgccgcaa
     4201 gtattgattg tcgatgagcc gacgcgcggc gtggatgtct cggcgcgtaa tgatatctac
     4261 caactgttgc gcagcatcgc cgcacagaat gtggctgtgc tgcttatctc ctccgacctg
     4321 gaagagatcg aactgatggc agatcgcgtg tatgtgatgc atcagggcga aattgcccac
     4381 tctgcactga ccgggcgcga tattaatgtc gagactatta tgcgcgttgc cttcggcgat
     4441 agtcagcgtc aggaggcgtc atgctgaagt ttattcagaa caaccgtgaa atcacggcac
     4501 tgctggcggt ggtgctgctg tttgtattac ccggttttct cgaccgccag tatttaagtg
     4561 tgcaaacgct gaccatggtt tatagcagcg cgcaaatcct gatcctgctg gcaatgggcg
     4621 cgacgctggt aatgcttacg cgcaatattg atgtttcagt gggttcgatt accggaatgt
     4681 gcgcggtgct gttggggatg ttactgaacg caggatattc actacctgtt gcctgtgtca
     4741 cgactttact gcttggtttg ctcgcgggat ttttcaacgg tgtcctggtc gcgtggctaa
     4801 agattcctgc cattgttgcc acccttggca cgttagggtt gtacagaggc atcatgttgc
     4861 tgtggactgg cggtaaatgg attgaagggt tacccgccga actgaaacag ctctccgcac
     4921 cgctgctgtt tggcgtttcc gcgattggtt ggttgacgat aattctggtg gcatttatgg
     4981 cctggctgct ggcgaagacg gcgtttggac gcagttttta tgccacgggc gataatttac
     5041 agggcgctcg tcaactgggc gttcgtacgg aagccattcg cattgtggca ttttcgttga
     5101 acggctgcat ggcggcactg gcgggaattg tgtttgcttc gcagattggt tttatcccca
     5161 accagaccgg taccgggctg gagatgaaag caattgcagc ctgcgtgctg ggcggcatta
     5221 gtttgctcgg cggttccggt gcgatcatcg gtgcggtact cggcgcatgg ttcctgacgc
     5281 agatcgatag cgtactggtg ctgttgcgca ttccggcatg gtggaacgat tttatcgcgg
     5341 gcctcgtgct gctggcggtg ctggtgtttg atggacgcct gcgttgtgcg ctggaacgta
     5401 atctacggcg gcaaaaatat gcccgcttta tgacgccacc gccatccgtt aaacccgctt
     5461 cgtcaggtaa aaaacgggag gccgcataat gcgtattcgc tacggttggg aactggctct
     5521 tgccgcactg ctcgttattg agattgtcgc atttggtgca attaacccgc ggatgttaga
     5581 tctcaatatg ttgctgttca gcaccagtga ctttatctgc attggcattg tcgccctacc
     5641 gctgacgatg gtgattgtca gtggcgggat cgatatttcg tttggttcga ccatcggcct
     5701 ctgcgccatt gcattgggcg tactgtttca aagtggtgtg ccgatgccgc tggcgatact
     5761 cctgacctta ctgctcggcg cattgtgcgg gctgatcaac gccggattaa ttatctatac
     5821 caaagttaac ccgctggtga ttacgcttgg cacgctgtat ctgtttgccg gaagcgctct
     5881 gctgctttcc ggtatggccg gagcgacggg gtacgaaggt attggtggat tcccgatggc
     5941 gtttacagat ttcgctaacc tggatgtgct gggactcccc gttccgctga ttatcttcct
     6001 gatatgtctc ctcgttttct ggctctggct gcataaaacc catgccggac gtaatgtgtt
     6061 tttgattggg caaagcccgc gcgtggcgct ttatagcgcg attccagtta accgcacctt
     6121 atgtgcgctc tatgccatga cggggctggc gtctgcggtc gccgctgtgc tgctggtatc
     6181 gtattttggt tcagcacgtt ccgatctcgg tgcgtcgttt ctgatgcccg ccatcaccgc
     6241 cgtggtgctt ggcggtgcca atatttatgg tggttccggt tccattatcg gcaccgccat
     6301 tgcggtttta ttagtgggat atttgcaaca aggtttgcaa atggcaggag tgccaaatca
     6361 ggtgtccagc gccctttccg gtgcgctact tatcgtcgtt gtcgtaggtc gttccgttag
     6421 cctgcatcgc cagcaaatta aagagtggct ggcgcgtcgg gccaataacc cattgccata
     6481 aaggatatct tcatgacact tcatcgcttt aagaaaatcg ccttacttag cgttcttggc
     6541 attgccgcaa tctctatgaa tgtgcaggcc gcagagcgta ttgcatttat tcccaaactg
     6601 gttggcgtgg gattttttac cagcggtggc aacggcgcac aacaagcggg taaagagctg
     6661 ggcgttgatg tgacctacga cgggccgaca gaacccagtg tttctggtca ggtacagttg
     6721 attaataact tcgtcaatca aggttataac gccattatcg tttctgcggt ttcgcctgat
     6781 ggcttgtgtc cggcactgaa acgcgccatg caacgtggtg tgagagtgct gacctgggac
     6841 tctgatacta aaccggagtg ccgctcttac tacattaatc agggaacgcc cgcccagttg
     6901 ggaggtatgt tggtggatat ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg
     6961 tttttctact caagccccac cgttacggac caaaaccagt gggtgaaaga agcgaaagcg
     7021 aaaatcgcca aagagcatcc tagctgggaa attgtcacta cgcagtttgg ctataacgat
     7081 gccactaaat cattacaaac cgcagaagga atattaaaag cgtatagcga tctcgacgcc
     7141 attatcgccc ccgatgccaa cgccctgccc gctgccgcac aagccgcaga aaacttgaaa
     7201 aatgacaaag tagcgattgt cggattcagt acgccaaacg tgatgcgtcc atatgtggaa
     7261 cgcggcacgg tgaaagaatt tggcctgtgg gatgtggttc agcaaggcaa aatttcagtg
     7321 tatgtcgcgg atgcattatt gaaaaaagga tcaatgaaaa cgggcgacaa gctggatatc
     7381 cagggcgtag gtcaggttga agtctcgcca aatagcgttc agggctatga ctacgaagcg
     7441 gatggtaatg gcatcgtact gttaccggag cgcgtgatat tcaacaaaga gaatatcggc
     7501 aaatacgatt tctgatgtgc attacttaac cggagtaagt tatggcagat ttagacgata
     7561 ttaaagatgg taaagatttt cgtaccgatc aaccgcaaca aaatatccct tttaccctga
     7621 aaggttgcgg tgcgctggat tggggaatgc agtcacgctt atcgcggata tttaatccga
     7681 aaacgggtaa taccgtgatg ctggcctttg atcatggtta ttttcagggg ccgactaccg
     7741 gacttgaacg cattgatata aatatcgccc cgctgtttga acatgccgat gtattaatgt
     7801 gtacgcgcgg cattttgcgc agcgtagttc ccccagcgac caataagccg gtggtactga
     7861 gggcgtcagg tgcgaactct attctggcgg aattaagtaa tgaagccgtg gcgttatcga
     7921 tggatgatgc cgtgcgcctg aacagttgcg cggtggcggc gcaggtttat atcggcagcg
     7981 aatatgaata tcagtcgatc aaaaatatta ttcagctggt tgatgccgga atgaaagtgg
     8041 gaatgccgac catggccgtg actggcgtgg gcaaagatat ggtgcgcgat cagcgttatt
     8101 tctcgctcgc gactcgaatc gccgctgaaa tgggggcgca aattatcaaa acctattatg
     8161 tcgaaaaagg ttttgaacgg attgttgccg gatgtccggt acccattgtt attgctggcg
     8221 gtaaaaaatt accggagcgc gaggcgctgg aaatgtgctg gcaggctatc gatcagggcg
     8281 cttctggtgt ggatatgggg cgtaatattt tccagtctga ccatccggtg gcgatgatga
     8341 aagccgtaca ggcggtggtt caccataacg aaacggctga tcgagcatat gaactctatc
     8401 tgagtgaaaa acagtaactg cggatctaag gagaagaatt atgcacgtca cactggttga
     8461 aattaacgtt catgaagaca aggttgacga gtttatcgaa gtttttcgcc agaaccacct
     8521 gggctctgta caggaagaag gcaatttgcg cttcgatgtc ttacaggacc cggaagtgaa
     8581 ttcgcgcttt tatatctacg aagcctataa agatgaagac gcagtggcgt tccataaaac
     8641 cacgccccac tacaaaacct gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa
     8701 aaaacgtctg ttcaatggtt tgatgccgtg aggcgaattt atcaatttta tctacaattg
     8761 gggtaacgcg ctgacgggag taaaaaaatg tctgactgga acccctcttt atatttgcac
     8821 ttttccgctg aacgatcgcg tccggcggtg gagctgcttg ccagagtgcc gctggaaaat
     8881 gtcgaatatg ttgccgatct tggctgtggc ccaggtaaca gcaccgccct tctacaacaa
     8941 cgttggcctg cggccaggat aacaggcatc gactcgtctc cggcgatgat tgctgaagcg
     9001 cgcagtgctt tgccagactg ccagttcgtg gaagcagaca tccgcaactg gcaaccggta
     9061 caggcactcg atctgatttt tgctaatgcc tcactgcaat ggctgcccga ccactacgaa
     9121 ttgtttcctc atctggtttc tttacttaat ccgcagggcg tgctggcagt acagatgcca
     9181 gataactggc tggagccgac ccatgtgctc atgcgcgaag ttgcctggga acaaaactac
     9241 ccggatcgcg ggcgtgagcc gttggctggc gttcatgctt actacgatat tttgagcgaa
     9301 gccggatgtg aggtcgatat ctggcgaacc acctactatc accagatgcc gtcacaccag
     9361 gcgattatcg attgggtaac tgccactgga ttacgtccgt ggttacagga tctgaccgag
     9421 agcgaacagc agctttttct taagcgctac catcagatgc tggaagagca gtatccactg
     9481 caagagaacg gacagatact gctggcattt ccgcgtctgt ttattgttgc ccggcgtatg
     9541 gagtaaatat cacgtcagct ggtaatgacg atcgggaaga atttttgctg gaatttcggc
     9601 ttcatcgttc atctgtaaca ggtcaatttc aatagcgttg cagatggcat ccagtggtaa
     9661 atcattgttt tcagtaccga acggatcttc cagttcttcc gccagacaat ccagcgaaat
     9721 aaaagtgtag gaaatcagca cagagataaa aggcgtcatg taatgcaggt ctacgaccag
     9781 cgcgaacggc agcatgatac aaaacagata aacggtacga tgcaaaatca gggtgtaggc
     9841 aaagggaatt ggcgtgtagg caatgcgctc acagcctgcc agaaccgctg aaatatcatt
     9901 aagccgatcg ttgaggctaa taaacaggat atctgaaagc tgcccattgc ggcgctgaac
     9961 cgccaaccac tcccccatta ttaacaagat acggttagct ggagagttcg aagccagtac
    10021 aggctgaaga tcttcagtct tgagataatg agccagcact tccgcctgtg gctgtttgcg
    10081 taatgtcatg cgtaaacagt gggcgaaggc gatttgcaac cgggcaaact cccttacact
    10141 tgccgaatcc ggcaatgtcg tttttacctc gcgcagtaac gaccgtgagg caatcatcaa
    10201 ctgcccccaa agttttcgcg cttcaacgta acgggcgtac ccggcattat tacgaaaacc
    10261 aagaaaaatg gcgatggcga caccgagaat gctgaacggt gcgagggtga atttgatgcc
    10321 cagatgcgtg taccagggca gcatgaaaat aacagcgata gaaaagagaa aattgagtag
    10381 taagcgcgat gatatcttgg ataatactga gccgtgccag acaaaaatac ggcgcagcca
    10441 gtgttgttgt ggacgaacaa tcatggttat cttcaggcgt gaaaaagtcg ccctattaaa
    10501 cgtgattacg atcacattct caagacgcta cttacaaatt acctacaagc ttacaacatt
    10561 aacaggcgct gcatgtggca tctgttaatg ataaaggcac ataggatgtt gtaactaata
    10621 tagtgaaata agaaacccgg tcgaaaccgg gttcagaagt aacggtgtta ttagcacaac
    10681 ggacgtacag cttcgcgcat ccctttttca agaatcgcat ccaggtcatt agcaacctgc
    10741 tcgaccagac caggcacttg cgtcaggtcc tgctcccagt ggtctttttc tgccaaaaca
    10801 atcgctacca gttcctgggt gccgataaca cggtcacgat gctggctcca gagttgctga
    10861 taacgttcca gccagtgtgc atcgtcctgt accggatatg tttctccgtt acgctcaccg
    10921 cgatagaagg caatcaatgc cgctaatgcg aaagtaaggc gcgccggaag tgtgcctttt
    10981 gccttctgcc ctgccagcag ctgcggcagg atgcgagtgc ggaacttggt cataccgttg
    11041 agcgcgatag acagcagctg atgcttaatg tacgggttac ggaaacgccc ggtgactgca
    11101 ctggcgaaag attccagttc atcacgaggc aaatccagta ccggaataat ttcttcgtag
    11161 atagcttttt caacgaatgc gcaaatttca gcatcgttca tcgcttcacc tacggtatcc
    11221 agccctgcct ggaacgccac cggcaccagc gcggtgtgcg caccgttgag gattgccact
    11281 ttgcgttctt tgtacggttt aatatcgtca acaatcagca cgttgagcgg atatttgtcc
    11341 agacgcagtt cagtcgctaa ggatttcggt ccctgaatca caaacaggta aaagtgttca
    11401 gcggtgtcga gaaaaccatc gtgataaccc agttcttctt ccagtttagc cacttcatcg
    11461 cgcggataac cggtaacgat acggtccacc
//
LOCUS       DAJOSY010000127        11282 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJOSY010000127 DAJOSY010000000
VERSION     DAJOSY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.127"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            363..1088
                     /locus_tag="OHN27_003745"
     CDS             363..1088
                     /locus_tag="OHN27_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCQ2288116.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            1046..1723
                     /gene="bglJ"
                     /locus_tag="OHN27_003746"
     CDS             1046..1723
                     /gene="bglJ"
                     /locus_tag="OHN27_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCQ2288117.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1761..2549)
                     /gene="fhuF"
                     /locus_tag="OHN27_003747"
     CDS             complement(1761..2549)
                     /gene="fhuF"
                     /locus_tag="OHN27_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCQ2288118.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2690..2926
                     /locus_tag="OHN27_003748"
     CDS             2690..2926
                     /locus_tag="OHN27_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCQ2288119.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2965..3051)
                     /locus_tag="OHN27_003749"
     tRNA            complement(2965..3051)
                     /locus_tag="OHN27_003749"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3015..3017),aa:Leu,seq:cag)
     gene            complement(3086..3172)
                     /locus_tag="OHN27_003750"
     tRNA            complement(3086..3172)
                     /locus_tag="OHN27_003750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3136..3138),aa:Leu,seq:cag)
     gene            complement(3201..3287)
                     /locus_tag="OHN27_003751"
     tRNA            complement(3201..3287)
                     /locus_tag="OHN27_003751"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3251..3253),aa:Leu,seq:cag)
     gene            complement(3596..4627)
                     /gene="rsmC"
                     /locus_tag="OHN27_003752"
     CDS             complement(3596..4627)
                     /gene="rsmC"
                     /locus_tag="OHN27_003752"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCQ2288120.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDSLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4730..5143
                     /gene="holD"
                     /locus_tag="OHN27_003753"
     CDS             4730..5143
                     /gene="holD"
                     /locus_tag="OHN27_003753"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCQ2288121.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            5112..5558
                     /gene="rimI"
                     /locus_tag="OHN27_003754"
     CDS             5112..5558
                     /gene="rimI"
                     /locus_tag="OHN27_003754"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCQ2288122.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5573..6250
                     /gene="yjjG"
                     /locus_tag="OHN27_003755"
     CDS             5573..6250
                     /gene="yjjG"
                     /locus_tag="OHN27_003755"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCQ2288123.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            6341..7930
                     /gene="prfC"
                     /locus_tag="OHN27_003756"
     CDS             6341..7930
                     /gene="prfC"
                     /locus_tag="OHN27_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCQ2288124.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            8323..8928
                     /gene="osmY"
                     /locus_tag="OHN27_003757"
     CDS             8323..8928
                     /gene="osmY"
                     /locus_tag="OHN27_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCQ2288125.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            9055..9216
                     /locus_tag="OHN27_003758"
     CDS             9055..9216
                     /locus_tag="OHN27_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCQ2288126.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            9338..10411
                     /locus_tag="OHN27_003759"
     CDS             9338..10411
                     /locus_tag="OHN27_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCQ2288127.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            10408..11190
                     /locus_tag="OHN27_003760"
     CDS             10408..11190
                     /locus_tag="OHN27_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCQ2288128.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         2779 a         2781 c         2980 g         2742 t
ORIGIN      
        1 gttaacaagt taaaatcatc agattttatt gaataaatat tgattgtgtg attcccgcat
       61 tctgcgggaa gggatatcat ttaattataa attaaatgaa tgtgatttct gtttttcatt
      121 aagtattcct aacaacttaa tgtagatatt ttaaatgtct ccaggctatt tcaccaggtt
      181 atcatttgat gctaaccttc tgacacatgc agtggagttg ttgtgcagca ggagtatgct
      241 gatatgaaag tgaatgctaa ggataattta ttcgcttaat ctattaattt gctggaaaca
      301 tttaaggaat gaatatcctg aataaaccat cataattttt agacagtgga tgtggaggaa
      361 atatgttgcc aggatgctgc aaaaatggaa ttgttatcag taaaatacct gttatgcaag
      421 cagggttaaa agaggtcatg aggactcact tccctgaata tgaaataata tccagcgcct
      481 ctgcggagga ccttacctta ttacaattac gtcgttccgg attagtcatt gctgatttag
      541 ccggtgaaag tgaagatcca cgttctgttt gtgaacatta ttattcttta atctcacaat
      601 atcgggaaat tcactgggtt ttcatggtgt cacgctcctg gtattcccag gcagtagaac
      661 tgctcatgtg ccctacggcg acgttattgt ctgatgttga acccattgag aatctggtca
      721 agaccgtacg ttccggcaat acgcacgcag agcgtatcag cgccatgctg acttctccgg
      781 caatgactga aactcatgat tttagctatc gctccgtcat tctcactctt tcagagcgca
      841 aggtactgcg gctattaggt aaaggatggg gcatcaacca gatagcttca ttgcttaaga
      901 aaagtaataa aactatcagc gcccaaaaaa acagtgcgat gcgtcgactg gcaattcaca
      961 gcaacgctga aatgtatgca tggataaata gcgcgcaggg tgcaagagaa cttaacttgc
     1021 cttctgttta tggagatgcc gcagaatgga acacagccga attaagaaga gaaatgtcgc
     1081 actcatagaa aaatgcgtca tgagtagtat cggtattgag agtttattca gaaagtttgc
     1141 gggtaaccct tataagctcc atacctatac cagtcaggag tcatttcagg atgccatgtc
     1201 gcggatctcg tttgcggcgg tcattttttc tttttctgcc atgagaagtg agcgcagaga
     1261 gggattatct tgcctgactg aactggcgat taagtttccg cgtacccggc gtttagttat
     1321 tgcggatgat gatattgaag ctcggctgat tggttcattg tcgccatcac cgctggacgg
     1381 tgtattaagt aaagcgtcaa cgctggagat ttttcatcag gaactctttt tgtcattaaa
     1441 tggtgtacgt caggcgaccg accgactgaa caatcagtgg tacattaacc aaagccggac
     1501 gctaagcccc acggagagag aaatattgcg ctttatgtcg cgtggctact caatgacaca
     1561 aattgccgag cagcttaaac gcaatatcaa aacgatccgt gcacataaat ttaatgtgat
     1621 gtcgaaactg ggcgtcagtt ctgatgcagg gttgttggag gccgcagata ttctgttatg
     1681 tatgcggcat tgcgaagcaa gtaatgtgtt gcatccctat taatccgcat gatgccgggt
     1741 ttacttcccc ggcagtgctt tcatttcagc gtacaatcgc cacattgctg cacatccggt
     1801 aagcgataac gctggcagca agtgcggcgc accagcaggc catcgcgcag taccacggta
     1861 cgccagagtg gattatcttc accgttcgtg agcgttttct caaaaaagag ggcatggcgc
     1921 agcgattcaa ccgtcgcctc gccgagcagc tgcttcatct cagtgagata ccagttgatc
     1981 aaataaccgg tattactcca gataagtttg ccgttgatct ctccggtcgc ttctagtgcc
     2041 tgcacaaccg gaaccagcgc ctggctgatt aacgtttcca ttcgctgctg cggcgaatgt
     2101 ggtgttgcgt ttttatcttc acacacatcg acccagaaac aggcgacgcg tccggtttcg
     2161 tgaaactcag catggaaatg ttccggcgac acatctaatg ccttttcctg cgtcagtagc
     2221 gccagcatta atggtggcac catcaggccg atataccatt gtgcccatag tgagatcagc
     2281 ggtttgttct cgcggatcat cgttggttgg ttgcgataga tatgatcgga atagaccgcg
     2341 agtagagaac ttagcgcatt cggtgatgac cattgcgcca gcgtcatggc gttaagcgga
     2401 gcgggttcat ccaggcggat aaactcgagc aaatgttcac gatgtttggc gatcgtcgca
     2461 cgtacggctt gtgcaagccc agcatcctgc ggctggagat gcgttcgcca gatgatatct
     2521 tcatagagcg gtgcggaacg ataggccata atcgggatag taatctaaat gataatgatt
     2581 gctaatccta gcgataggtt tacccgatag caagggattt ctctggcttg caaatgataa
     2641 aaattatcat atgatattgg ttatcattat caatgaaaga gatgaaacca tgttgcaacg
     2701 tacgctgggc agtggctggg gagtgttgct gccgggattg ctgattgcag ggctgatgta
     2761 tgcggattta tcgccggatc agtggcggat tgtcattctg atgggattag tattgacgcc
     2821 ggtgatgctg tatcacaaac agttacggca ttacgttttg ctaccatcgt gcctggcgct
     2881 tattgctgcc atcatgctga tgataatgaa tttgaatcag ggatgaaaaa tcaaggaaga
     2941 aacaagaaag gaagtaaaga taattggtgc gagggggggg acttgaaccc ccacgtccgt
     3001 aaggacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg cacagtcatc
     3061 ttactttttt tgatatcgcc tcgtttggtg cgaggggggg gacttgaacc cccacgtccg
     3121 taagaacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc gcccagtgcg
     3181 agcaatatca acgtggtttt tggtgcgagg ggggggactt gaacccccac gtccgtaagg
     3241 acactaacac ctgaagctag cgcgtctacc aattccgcca ccttcgcata ccatcaattc
     3301 ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt cgtcaatagt
     3361 taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta acgtgcaggt
     3421 ttggtagttt tgtaggtcgg ataaggcgtt cacgccgcat ccgacacggt atttgggtgc
     3481 agatgccaga tgcgacgcgg gcgcgtctta tctggcctac gaagggctaa cgtgcaggtt
     3541 ttgtaggtcg gataaggcgt tcacgccgca tccgacacgg tattcggcga gataattaac
     3601 ctttcttcgc ctggcgggtc ataatggcgc gatacacctt gaaacgcccg gtttgcgcaa
     3661 tcacttcatg gaagccaaat gtctcatcca gcacgtccgg gtaaggcagg aaggcgttcg
     3721 ctacaattcg cagctcgccg ccgctattaa gatgacgcac cgcgccgcga atcagcgttt
     3781 gcgccgcatc caggctggtt tgcatcccat cgtggaacgg cgggttggag atgatcatat
     3841 caaaacgacc tttcacctcg gaaaagacgt tgctggcaaa gacttcacct tcaacaccgt
     3901 tggccgcaag tgttgcgcgg ctggcttcta ccgctggcgc agagacatcg cacaaggtga
     3961 gacgaatttt cggcgaatgg cgcgcaaagg caactgaaag cacacccgcg ccacagccga
     4021 catccagcac tttacctttc gtgtgcgggg ttaacgtcga gagcagcaac tggctaccga
     4081 catccagact gtcgcggcta aacacgccag gcagcgtttt gaccgtcagg ccatcgacgc
     4141 tgtattcgcc ccagaattta tcggcatcaa ataccggctg tttttccaga cgaccaaaat
     4201 agaggccaca gcgacgagcg ctgtcgactt tattcaacgg cgcataatct gccagcatct
     4261 gctcggcgct gcgcacgccg ctgcggttct cgccaacgac aaaaatatct gtccccactg
     4321 gcagcagaga aagtaaattc atcaactgga actgggcttc cggtttgttc ttcggccagt
     4381 agtaaatcag cgtatcgcaa tctgcgacgt catccgccgt ggcgaccaga ctgaaacggg
     4441 cgttatcccc catctggcgg cttaatacct gccagtggtg gaattgctgg gtatgagcac
     4501 ggctggccgc ggtatctaaa cgcgcgggca ggtcatcctg taagtctccg gcaaacagaa
     4561 tacggctttg ttcgaaatca tcactgtgac gcagcaagac ttcacttgcc ggggtaaatg
     4621 cagacatgga atgctcctca attgatactg gcggcgatta tagccatatg ttggcgcggt
     4681 atcgacgaat ttgctatatt tgcgcccctg acaacaggag cgattcgcta tgacatcccg
     4741 acgagactgg cagttacagc aactgggcat tacccagtgg tcgctgcgtc gccctggcgc
     4801 gttgcagggg gagattgcca ttgcgatccc ggcacacgtc cgtctggtga tggtggcaaa
     4861 cgatcttccc gccctgactg atcctttagt gagcgatgtt ctgcgcgcat taaccgtcag
     4921 ccccgatcaa gtgctgcaac tgacgccaga aaaaatcgcg atgctgccgc aaggcagtcg
     4981 ctgcaacagc tggcggttgg gtactgacga accgctatca ctggaaggcg ctcaggtggc
     5041 atcaccggcg ctcaccgaat tacgggcaaa cccaacggca cgcgccgcgt tatggcaaca
     5101 aatttgcaca tatgaacacg atttcttccc tcgaaacgac tgatttacca gcggcttacc
     5161 acattgaaca acgcgcccac gcctttccgt ggagtgaaaa aacctttgcc agcaaccagg
     5221 gcgagcgtta tctcaacttt cagttaacgc aaaacggcaa aatggcggcg tttgcgatta
     5281 cgcaagtggt gctggatgaa gctacattgt tcaatattgc ggtcgatcct gactatcagc
     5341 gtcagggatt gggaagggcg ctgctggaac atctgatcga tgaactggaa aaacgcggcg
     5401 tggcgacgct ctggctggaa gtccgtgcct caaacgctgc cgccattgcc ctgtacgaaa
     5461 gtttaggctt taacgaggcg acgattcgcc gcaattacta ccccaccacg gacggtcgcg
     5521 aagacgccat catcatggcg ttgccaatca gtatgtaaga caaggtggaa taatgaagtg
     5581 ggactggatt ttctttgatg ccgatgaaac gctgtttacc tttgactcat tcaccggcct
     5641 gcagcggatg tttcttgatt acagcgtcac ctttaccgct gaagattttc aggactatca
     5701 ggccgttaac aagcctctgt gggtggatta tcaaaacggc gcgatcactt cattacagct
     5761 tcagcacggg cgtttcgaga gctgggccga acggctgaac gtcgagccgg gtaaactcaa
     5821 cgaagccttt attaatgcga tggcggaaat ctgcacgccg ctgccgggcg cggtttctct
     5881 gcttaacgcc attcgtggca acgccaaaat cggcatcatc accaacggct ttagtgcctt
     5941 gcaacaggtg cgtctggaac gcacgggcct gcgtgattac ttcgatttgc tggtgatttc
     6001 cgaagaagtt ggcgttgcca aaccgaataa gaaaattttc gattatgcgc tggaacaggc
     6061 gggcaatcct gaccgttcac gcgtgctgat ggttggcgac actgccgagt ccgatattct
     6121 cggtggcatc aacgccgggc ttgcgacctg ctggctgaat gcgcacaatc gcgagcaacc
     6181 agaaggcatc gcgcccacct ggaccgtttc ttcgttgcac gaactggagc agctcctgtg
     6241 taaacactga ttgcctcccc cccgttgatg ggtaaaatag ccgcaatttt tcgttttcaa
     6301 caagcgcggc gcgatgccgc ttactcaaga agaaagaatt atgacgttgt ctccttattt
     6361 gcaagaggtg gcgaagcgcc gcacttttgc cattatttct cacccggacg ccggtaagac
     6421 taccatcacc gagaaggtgc tgctgttcgg acaggccatt cagaccgccg gtacagtaaa
     6481 aggccgtggc tctaaccagc acgctaagtc ggactggatg gagatggaaa agcagcgtgg
     6541 gatctccatt actacgtctg tgatgcagtt tccgtatcac gattgcctgg ttaacctgct
     6601 cgacaccccg gggcacgaag acttctcgga agatacctat cgtaccctga cggcggtgga
     6661 ctgctgtctg atggttatcg acgccgcaaa aggtgttgaa gatcgtaccc gcaagctgat
     6721 ggaagttacc cgtctgcgcg acacgccgat cctcaccttt atgaacaaac ttgaccgtga
     6781 tatccgcgac ccgatggaac tgctcgatga agttgagaac gagctgaaaa tcggctgcgc
     6841 accgatcacc tggccgatcg gctgcggcaa gctgtttaaa ggcgtttacc acctttataa
     6901 agacgaaacc tatctctatc agagcggtaa aggccacacc attcaggaag tccgcattgt
     6961 taaagggctg aataacccgg atctcgatgc agcggtcggt gaagatctgg cacagcagct
     7021 gcgtgacgaa ctggaactgg tgaaaggcgc gtctaatgag ttcgacaaag agctgttcct
     7081 tgcgggcgaa atcactccgg tattcttcgg tactgcgctg ggtaacttcg gtgtcgatca
     7141 tatgctggat ggcctggtgg agtgggctcc tgcgccgatg ccgcgtcaga ctgatacccg
     7201 taccgtagag gcgagcgaag acaaatttac cggcttcgta tttaaaattc aggccaacat
     7261 ggacccgaaa caccgcgacc gcgtggcgtt tatgcgcgtg gtatccggta aatatgaaaa
     7321 aggcatgaag ctgcgccagg tgcgcactgc gaaagatgtg gtgatctccg acgcgctgac
     7381 ctttatggca ggtgaccgtt cgcacgttga agaagcatat ccgggcgata tcctcggcct
     7441 gcacaaccac ggcaccattc agattggcga cacctttacc cagggtgaga tgatgaagtt
     7501 caccggtatt ccgaacttcg cgccagaact gttccgtcgc atccgcctga aagatccgct
     7561 gaagcaaaaa cagctgctca aagggctggt acagctttcc gaagagggcg cggtgcaggt
     7621 gttccgtcca atctccaaca acgatctgat cgttggtgcg gttggtgtgc tgcagtttga
     7681 tgtggtggta tcgcgcctga agagcgaata caacgttgaa gcagtatatg aatcagtcaa
     7741 cgttgccacc gcccgctggg tagaatgtgc agacgcgaag aaattcgaag agttcaagcg
     7801 taagaacgaa agccaactgg cgcttgatgg cggcgataac ctcgcttaca tcgctaccag
     7861 catggtcaac ctgcgcctgg cgcaggaacg ttatccggac gttcagttcc accagacccg
     7921 cgagcattaa ttcttgcctc cagggcgcgg tagccgctgc gccctgtcaa tttcccttcc
     7981 ttattagccg cttacggaat gttcttaaaa cattcacttt tgcttatgtt ttcgatgata
     8041 tcccgagagg tttcaaaatt gtgatctata tttaacaaag taatgacatt tctgacggcg
     8101 ttaaataccg ttcaatgcgt agatatcagt atctaaagcc gtagattgtc attctaccga
     8161 tattaataac tgattcagag gctgtaatgg tcgttattca tcacccatcg cttttgtgat
     8221 ggcgaccatt gacttctgta gagggtgaag tctctcccta ttcagcaatg caacctcgtg
     8281 ttgccaggct caaattacga gcaaacttac aggaataaat cgatgactat gacaagactg
     8341 aagatttcga aaactctgct ggctgtaatg ttgacctctg ccgtcgcgac aggctctgcc
     8401 tacgcggaaa acaacgcgca gactaccaat gaaagcgcag ggcaaaaagt cgatagctct
     8461 atgaataaag tcggtaattt catggatgac agcgccatca ccgcgaaagt gaaggcggcc
     8521 ctggtggatc atgacaacat caagagcacc gatatctctg tcaaaaccga tcaaaaagtc
     8581 gtgaccctga gcggtttcgt tgaaagccag gctcaggccg aagaggcagt gaaagtggcg
     8641 aaaggcgttg aaggggtgac ctctgtcagc gacaaactgc acgttcgcga cgctaaagaa
     8701 ggctcggtga agggctacgc gggtgacacc gccaccacca gtgaaatcaa agccaaactg
     8761 ctggcggacg atatcgtccc ttcccgtcat gtgaaagttg aaaccaccga tggcgtggtt
     8821 cagctctccg gtaccgtaga ttctcaggca caaagtgacc gtgctgaaag tatcgccaaa
     8881 gcggtagatg gtgtgaaaag cgttaaaaat gatctgaaaa ctaagtaatt cgtcgtaatt
     8941 cgtcctcccg aactttgtcg ggaggcgcga tgtgcaccac actaaaaata tcgcattgag
     9001 tgattttcac gctcatatca agcggtgaca ttaactatgg taaaggagac gcttatgttt
     9061 cgttggggca tcatatttct ggttatcgcg ttaatcgccg ccgcacttgg gtttggtggt
     9121 ctggccggta ccgctgcagg cgcagctaaa attgtctttg tcgtcgggat tattctgttc
     9181 ctggtgagtt tgttcatggg ccgaaaacga ccctagattt cagaccatta agaaaatttt
     9241 atccaaagcc agtccagcgg actggctttt gcggttttag cgaataataa attgcgttac
     9301 tttgtcattc tttcacaaca aaaacaggaa ggcagaggtg gggcagcgaa tacctgtaac
     9361 gcttggtaat attgcgccgt tgtcgctaag gccgttccag cctggacgaa tagctctggt
     9421 gtgcgaaggc ggcggacagc gtggaatttt cacggctggc gtgctggatg agtttatgcg
     9481 cgcgcagttt aatcctttcg atctttatct cggcacatct gccggggcgc agaacctctc
     9541 ggcgtttatc tgcaatcagc ccggttacgc gcgcaaagtc atcatgcgct ataccacaaa
     9601 acgcgaattt ttcgatccat tgcgctttgt ccgtggagga aatcttatcg atctcgactg
     9661 gctggtggag gccaccgcaa gccagatgcc gttgcaaatg gataccgccg cgcggttgtt
     9721 tgacagcggc aaatcgtttt atatgtgcgc ctgtcgtcag gatgactacg cgcctaatta
     9781 ctttttacca accaaacaaa actggctgga tgtgattcgc gcctccagtg cgatacctgg
     9841 cttttatcgt agcggagtgt cgctggaagg cattaactac ctggatggcg ggatcagtga
     9901 tgcgattccg gttaaagagg cggcaaggca gggcgctaaa acgttggtcg tcatccgcac
     9961 tgtgccgtca caaatgtact acacgccgca gtggttcaaa cgcatggaac gctggctggg
    10021 tgacagtagc ctgcagccgc tggtcaatct ggtgcagcat catgaaacca gctatcgtga
    10081 cattcagcaa tttattgaga aaccaccggg caagctgcgg atattcgaaa tttatccacc
    10141 gaaaccatta catagtatcg cgcttggcag tcggattccg gcgctgcgtg aagactataa
    10201 acttgggcgt ttatgcggtc gttatttcct cgccacggtt ggcaagctat taactgaaaa
    10261 agcgccgctt acccgccatc tggtgccagt ggtgacgccg gaatcgatcg ttattccgcc
    10321 tgcgccagtc gccaacgata cgctggttgc cgaagtgagc gatgctccgc aggcgaacga
    10381 cccgacattt aacaatgagg atctggcttg atttgccgtt ttatcgatac ccactgccat
    10441 tttgatttcc cgccgtttag tggcgatgaa gaggccagcc tgcaacgcgc ggcacaagcg
    10501 ggcgtaggca agatcattgt tccggcgaca gaggcggaaa attttgcccg cgtgcaggcg
    10561 ttagcggaaa aatatcaacc gctgtatgcc gcattggggt tgcatccggg gatgttggaa
    10621 aaacatagcg atgtgtctct tgatcagcta cagcaggcgc tggaaaggcg tccggcgaag
    10681 gtggtggcgg tgggggagat cggtctggat ctcttcggcg acgatccgca atttgagagg
    10741 cagcagtggt tactcgacga acagctgaaa ctggcgaaac gctacgatct gccggtgatc
    10801 cttcattccc ggcgtacaca cgacaaactg gcgatgcatc ttaaacgtca cgatttaccg
    10861 tgcactggcg tggttcacgg tttttccggc agcctgcaac aggccgagcg gtttgtacag
    10921 ctgggctaca aaatcggcgt aggcggtact atcacctatc cacgcgccag taaaacccgc
    10981 gatgtcatcg caaaattacc gctggcatcg ttgttgctgg aaaccgacgc gccggatatg
    11041 ccgctcaacg gctttcaggg gcagcctaac cgaccggaac aggctgtgcg ggtgtttgac
    11101 gtgctttgcg agttgcgccc ggaaccagaa gatgagattg ccgaagtgtt gcttaataat
    11161 acgtatgcgt tgtttagcgt tagtgggtag tttacccttc gtaggcagca tccggcagtc
    11221 ggcgcataat gcctgatgcg acgcttgtcg cgtcttatca tgcctacagg tttgtgccga
    11281 ac
//
LOCUS       DAJOSY010000128        11128 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJOSY010000128 DAJOSY010000000
VERSION     DAJOSY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.128"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..729
                     /gene="ydeO"
                     /locus_tag="OHN27_003761"
     CDS             <1..729
                     /gene="ydeO"
                     /locus_tag="OHN27_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCQ2288129.1"
                     /translation="HAFNANILDKDYAFSDGEILMVDNAVRTNFEPYERHFKEIGFNE
                     NTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFS
                     CLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKL
                     KQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKR
                     VSKEYRCQSHTGMNTGNTMNALAI"
     gene            1131..2813
                     /locus_tag="OHN27_003762"
     CDS             1131..2813
                     /locus_tag="OHN27_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCQ2288130.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2865..4022
                     /locus_tag="OHN27_003763"
     CDS             2865..4022
                     /locus_tag="OHN27_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCQ2288131.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3988..4110)
                     /locus_tag="OHN27_003764"
     CDS             complement(3988..4110)
                     /locus_tag="OHN27_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288132.1"
                     /translation="MTTHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            4313..5998
                     /locus_tag="OHN27_003765"
     CDS             4313..5998
                     /locus_tag="OHN27_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCQ2288133.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWESAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            6036..8407
                     /locus_tag="OHN27_003766"
                     /pseudo
     CDS             6036..8407
                     /locus_tag="OHN27_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            8464..>11128
                     /locus_tag="OHN27_003767"
     CDS             8464..>11128
                     /locus_tag="OHN27_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCQ2288134.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYVFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLPTRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPA"
BASE COUNT         3273 a         2436 c         2415 g         3004 t
ORIGIN      
        1 catgctttca acgctaacat tttagataaa gattacgcct tctctgacgg cgagatcctg
       61 atggtagata acgctgttcg tacaaatttt gaaccttatg agcggcattt taaagaaatc
      121 ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg
      181 gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa
      241 atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga acttttgctt
      301 ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt
      361 gtgctatccg tttctgggaa agtgagaaat attgtcaaca tgaagctggc gcacccatgg
      421 aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaactt
      481 aagcaagagc aaacgacatt ctcacagatt cttttagatg caagaatgca gcacgcaaaa
      541 aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt
      601 acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa gagagtttct
      661 aaggagtacc gttgtcaaag tcacacgggt atgaatacgg gcaacacgat gaatgcttta
      721 gctatttgat tatttgctaa cgagtagtca accacacacg ctgcgtaaga attaaatggg
      781 gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat aaataagtga
      841 catccatcac atatttatgc acttgcataa cctgttgcat gattatttat gatctcaatt
      901 ctgcattttg tcagtaaaat gcaataattt attaaatatc aataaattag ttgtttatcg
      961 gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact gttttttccc
     1021 tttttttagc taaatctgct atctctttat gtgactaact tcacttacat ccacttattt
     1081 ctcttcgtaa aattactttg gaattaagta caataagaag aggaacattt atgaagtctg
     1141 cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct ggtatggctg
     1201 catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca aacgttgctt
     1261 tctcagactt tacgccgaca gaatacagta ccaaaggaaa gccaaatatt atcgtactga
     1321 ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa
     1381 caatggaaaa tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag
     1441 ctgcacaaaa atcaacgccg acgctccttt cattaatgga tgaaggcgta cgttttacta
     1501 acggctatgt ggcacacggt gtttccggcc cctcccgcgc cgcaataatg accggtcgag
     1561 ctcccgcccg ctttggtgtc tattccaata ccgatgctca ggatggtatt ccgctaacag
     1621 aaactttctt gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat
     1681 ggcacttgtc aaaaatcagt aatgtgccgg taccggaaga taaacaaacg cgtgactatc
     1741 atgacaactt caccacattt tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt
     1801 actttatggg attccacgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa
     1861 atcgtgaacg tgtccccgca aaaggttata tcagcgatca gttaaccgat gaggcaattg
     1921 gcgttgttga tcgtgccaaa acacttgacc agccttttat gctttacctg gcttataatg
     1981 ctccgcacct gccaaatgat aatcctgcac cggatcaata tcagaagcaa tttaataccg
     2041 gtagtcaaac agcagataac tactacgctt ccgtttattc tgttgatcag ggtgtaaaac
     2101 gcattctcga acaactgaag aaaaacggac agtatgacaa tacaattatt ctctttacct
     2161 ccgataatgg tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa aaaggctata
     2221 agagtcagac ctatcctggc ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac
     2281 ttcaacccgg taattatgac aagctgattt ccgcaatgga tttctacccg acagctcttg
     2341 atgcagccga tatcagcatt ccaaaagacc ttaagctgga tggcgtttcc ttgctgccct
     2401 ggttgcaaga taagaaacaa ggcgagccac ataaaaatct gacctggata acctcttatt
     2461 ctcactggtt tgacgaggaa aatattccat tctgggataa ttaccacaaa tttgttcgcc
     2521 atcagtcaga cgattacccg cataacccca acactgagga cttaagccaa ttctcttata
     2581 cggtgagaaa taacgattat tcgcttgtct atacagtaga aaacaatcag ttaggtctct
     2641 acaaactgac ggatctacag caaaaagata accttgccgc cgccaatccg caggtcgtta
     2701 aagagatgca aggcgtggta agagagttta tcgacagcag ccagccaccg cttagcgagg
     2761 taaatcagga gaagtttaac aatatcaaga aagcactaag cgaagcgaaa taactaaacc
     2821 ttcatgcggc ggatttttcc gccgccttat tgagcgagat agcgatgcac gttacagcca
     2881 agccctccag ttttcaatgt aatctcaaat gtgattactg tttttacctt gaaaaagagt
     2941 cgcagtttac tcatgaaaaa tggatggatg acagcactct gaaagagttc atcaaacaat
     3001 atatcgcagc gtctggcaat caggtctatt ttacctggca aggcggtgaa cccactctgg
     3061 ctggcctgga ttttttccgt aaagttattc actatcaaca acgctatgca ggccaaaaac
     3121 gtatttttaa tgcattacaa acgaatggca ttttattgaa taatgaatgg tgtgcctttc
     3181 tcaaagaaca tgaatttctg gttggtatct cgatcgatgg cccccaggag ttacatgacc
     3241 gttacagacg cagtaattca ggtaacggta cttttgcaaa agtgatagca gccatcgagc
     3301 gtctgaaatc atatcaagta gagtttaata cgttaaccgt cattaataac gttaatgtcc
     3361 attaccctct tgaggtttat cattttttaa aatctatcgg cagtaaacat atgcaattta
     3421 tcgaattgct agaaaccggg acgccgaata ttgatttcag tggtcatagt gagaacacat
     3481 tccgtatcat tgatttttct gtgcctccca cggcttatgg caagtttatg tcaaccattt
     3541 ttatgcaatg ggttaaaaac gatgtgggtg aaattttcat tcgtcagttt gaaagctttg
     3601 tcagccgttt tttggggaat gggcatacaa gttgtatttt ccaagagtcc tgcaaggata
     3661 atctggttgt tgaaagtaat ggagacattt acgaatgcga ccattttgtc tatccacagt
     3721 acaaaattgg aaacattaat aaatctgaac tcaaaacgat gaacagtgta caactgacag
     3781 cgcaaaaaaa acggatttca gcgaaatgtc tgaaatgtgc atataaacct atctgcaatg
     3841 gcggttgtcc taagcatcgt attactaaag taaacaatga gactgtttct tatttttgcg
     3901 aaggttataa aatccttttt tcaaccatgg taccttatat gaacgccatg gtggagttag
     3961 ctaagaacag agtaccgctt taccacatta tggatgttgc aaaacaaatg gagaataatt
     4021 aataacgctc tcttttagct ccattcttag cctccttctt atgaccttaa caaggcaccc
     4081 gacataaatt cctgtcgggt gtgtcgtcat catcatcttc ccttgcgaaa accctggctt
     4141 acgtggtatg cgtcagggca ttagcaatac ccttgattat caatagcata accataacca
     4201 ctgaaaatta aaaatttcta tatatttcat attgttaaat gatacttaaa taaatttcat
     4261 cagtaaatca ttgccataga taatcattgg atggcacaat caattttcgc taatgataac
     4321 cattcccatt acgttttgta tgttgatagc aaagtatctc tgtctgctga agcccttttg
     4381 gctgcgtaag aataataaaa cttcagtgtt gttaatcatt attatcctcg ccatgatcct
     4441 tggcgtggtt aaaattcagg tctggttaaa tgactggaat aatgattttt tcaatgcgct
     4501 aagccagaaa gaaaccgata agctttggca gcttgtcctt tggttccctg ccctgctggg
     4561 gatctttgta ttaatatccg taaacaaaac atggttaatt aaactactga ctattcgctg
     4621 gcgcgaatgg ttaacagatt actaccttaa tcggtggttt gcagataaaa actactactt
     4681 cacgcagatc tacggcgagc ataaaaatac agataaccct gaccaacgta tcgctgaaga
     4741 cattctctta ctgatcagca aaacgctcag tctttcattt ggcttcatcc agtcacttag
     4801 catgctgatc acctttaccg ttattctttg ggaaagcgcg ggtacgctct cattcactgt
     4861 tggtggaaca gaatggaata tccaggggta tatggtctat accgtcgtgc tcattgtgat
     4921 cggtggaact ttatttaccc ataaggtggg taaacgtatt cgtccgctta atgtggaaaa
     4981 acaacgtagc gaagcgacat ttcgaacaaa tcttgtgcag cataataagc aggcagaact
     5041 gattgcgcta agcaatgctg agtctctaca acgccaggag ttgagcgaca attttcatac
     5101 cattaaagag aactggcatc gtttaatgaa tcgtcagcgg tggcttgatt actggcagaa
     5161 tatttattcg cgctcgctta gcgttcttcc ctactttctg ttattgccgc agtttattag
     5221 cgggcagatc aatctgggcg gactgatgaa atcgcgccag gcatttatgc tggtatcgaa
     5281 caatttaagc tggtttattt ataaatatga cgaacttgct gaactggctg cggttatcga
     5341 tcgcttgtat gagttccatc aactcactga acagcgccct acgaataagc ctaaaaattg
     5401 ccaacatgcg gtacaagtgg ctgatgcgag tattcgtacg cctgataata agatcatatt
     5461 agagaacctg aactttcatg tttcgccagg caaatggcta ttactgaaag gctactctgg
     5521 tgcgggaaaa accacactgc ttaaaacatt atcccactgc tggccgtggt ttaaaggtga
     5581 tatttcttct cctgctgaca gttggtatgt gtcacaaaca ccgttaatca aaaccggctt
     5641 actgaaagag attatttgta aagcacttcc cctgcccgta gacgataaat cgttgagcga
     5701 agtactgcat caggttggtc ttgggaaatt ggctgcgcgt attcatgacc acgatcgctg
     5761 gggagatatt ctttccagcg gcgaaaaaca acgtatcgcc ctggcacgat taattttacg
     5821 acgtccgaaa tggatatttc ttgacgaaac tacctctcat cttgaggaac aagaggctat
     5881 ccgcttactg cgtttagtgc gtgaaaaact acccacaagc ggcgtcatta tggttacaca
     5941 tcaacccggt gtctggaacc tggccgatga tatttgtgac attagcgcgg ttttataaaa
     6001 acaataaaag ccctgctgta cggagatata aataaatgaa gcgagttctt attcctggcg
     6061 tcattttatg tggcgctgat gtggcgcagg ccgtcgatga caaaaacatg tacatgcatt
     6121 tttttgaaga gatgacggtc tatgctcctg tccctgtacc cgtaaacggc aacacgcatt
     6181 acaccagtga aagcatcgag cgtttaccga ccgggaatgg caatatcagc gatctgctga
     6241 gaaccaaccc tgcggtacgc atggattcaa cgcaaagtac ctcgttgaac cagggagata
     6301 ttcgccctga gaaaatctct attcacggtg cgtcgcccta ccagaatgcc tatttgattg
     6361 acggtattag tgcaactaat aacctgaacc cagcgaatga gtccgatgcc agtagtgcaa
     6421 ccaatattag cgggatgtca caggggtatt atcttgatgt cagcttactg gacaatgtga
     6481 cgctttatga cagttttgtg ccggttgaat ttggtcgctt caatggcggg gtaattgatg
     6541 caagatcaaa cgcttcaacg ctgatgatag caaggtgaaa ttgggttatc gcactacgcg
     6601 ttcggactgg ttaacatcgc atatcgatga gaataacaag agcgcattta atcaaggctc
     6661 ttcaggaagt acttattact ccccagattt taaaaagaac ttttatacct tgtcgtttaa
     6721 tcaggaactc gctgataact tcggcgttac cgccggttta tcgcgccgcc agtctgatat
     6781 cacccgcgcg gattatgttt cgaatgacgg cattgtcgcc ggtcgggcac agtataaaaa
     6841 cgttatcgat actgcattaa gcaaatttac ctggtttgcc agcgaccgct ttacccacga
     6901 tttaacctta aaatataccg gctccagccg tgattataat accagcacct tcccgcagtc
     6961 tgatcgcgaa atgggtaata aatcctatgg tctggcatgg gatatggata cgcagctcgc
     7021 atgggccaaa ctgcgtacca ccgttggttg ggatcatatt agtgattata cccgtcacga
     7081 tcatgacatc tggtacaccg aactttcatg tacatatggt gatattacag ggcgttgcac
     7141 ccgtggcgga ttaggacaca tttcccaggc tgtagataat tacaccttca aaacacgcct
     7201 ggactggcaa aaattcgccg tgggtaatgt ttcgcatcaa ccctacttcg gcgcggaata
     7261 catctattcc gatgcgtgga ctgaacgcca taaccagtct gaatcctatg tgattaatgc
     7321 tgccggaaag aaaactaacc ataccattta ccataaaggt aaaggccgcc tgggaattga
     7381 caactacacg ctgtatatgg cggatcgcat tagctggcgt aatgtgtcat taatgcccgg
     7441 cgtgcggtat gactatgaca actatctgtc aaaccacaat atctccccgc gctttatgac
     7501 ggaatgggat atttttgcta atcaaacctc aatgattaca gcaggttata accgttacta
     7561 tggcgggaat attcttgata tgggattacg tgatatccgt aatagctgga cggaatcggt
     7621 atcaggtaat aaaactctga cgcgttatca ggatttgaaa acgccttata acgatgaact
     7681 ggcaatggga ttgcagcaga aaatcggtaa gaacgttatt gcgcgtgcaa actatgttta
     7741 ccgtgaagcg catgatcaaa tcagcaaaag cagtcgtacc gacagcgcga ctaaaaccac
     7801 cattactgaa tataacaacg atggcaaaac caaaacgcat tcgttcagcc tcagttttga
     7861 actggccgaa cccctgcata tccgccaggt agatattaac ccacaaattg tctttagcta
     7921 tatcaagagc aagggcaact tgtcgttaaa caatggttat gaggagagca ataccggtga
     7981 taaccaggtg gtttataacg gtaatctggt ctcttacgat agcgttccag tggcagattt
     8041 taataaccca ttaaagatct ccttaaacat ggatttcacg catcaaccga gcgggttagt
     8101 gtgggcgaat acgctggcct ggcaagaagc gcgtaaagct cgcattatcc ttggtaagac
     8161 aaatgcgcaa tacatcagcg aatattcaga ttacaagcag tatgttgacg aaaaactgga
     8221 tagcagcctg acctgggaca cccgcttgtc ctggacgcca caatttctga aacaacaaaa
     8281 cctgacgatc agtgccgata ttctcaatgt actggatagc aaaaccgctg ttgatacaac
     8341 gaacaccggt gtggcgacct acgccagtgg ccgtactttc tggcttgatg tcagcatgaa
     8401 attttaatag ttcggatttt acttaaccgg gtaaattcgc ccggttttcg catggagata
     8461 attatgagaa acctctgttt cttactgacg ttagtggcaa ctctgttgct ccccgggcgg
     8521 ctgattgccg ccgccttacc gcaggatgaa aagttaatta ccgggcaact ggacaatggc
     8581 ttgcgatata tgatttatcc gcatgctcat ccaaaggatc aggtaaattt atggctgcaa
     8641 attcataccg gttcattgca ggaagaagac aatgagcgcg gcgtggctca ttttgtagaa
     8701 catatgatgt ttaacggcac aaaaacatgg ccgggtaata aagtcatcga aacatttgag
     8761 tcaatgggcc tgcgttttgg tcgcgatgtt aatgcctata ccagctatga cgaaacggtg
     8821 tatcaggtga gtttgccgac tacgcagaaa caaaatctgc aacaagtgat ggcaatcttc
     8881 agtgaatgga gtaatgccgc cacctttgaa aaactcgaag tagacgctga acgtggcgta
     8941 attactgagg aatggcgtgc ccatcaggat gcgaaatggc gcacctctca ggcgcgccgc
     9001 cctttcctgc tggctaatac ccgtaattta gaccgtgaac ctatcggcct gatggatact
     9061 gtcgccacgg tcacaccggc acaattgcgc caattttatc aacgctggta tcaacccaat
     9121 aatatgacct ttatcgtggt cggcgatatc gacagtaaag aagcgctggc gctgataaag
     9181 gataatttaa gtaagcttcc ggctaacaaa gcagctgaaa atcgcgtctg gccgacaaaa
     9241 gctgaaaatc acctgcgctt taatatcatt aatgataaag aaaaccgggt gaacggcatc
     9301 gcactctatt atcgcctgcc aatggtacaa gtgaacgatg agcaaagctt tatcgaacaa
     9361 gctgaatgga gcatgttagt tcagctgttc aatcaacgtc tgcaggaacg catacagtcg
     9421 ggcgagttga agactatttc tggcggcact gcgcgcagcg ttaaaattgc acccgattat
     9481 cagtcgctgt ttttccgtgt aaatgcacga gacgataata tgcaggatgc tgcgaatgca
     9541 ttaatggcag agttggcaac cattgatcag catggttttt ctgctgaaga actcgatgat
     9601 gtcaaatcta cccgcctcac ctggctgaaa aatgcggttg atcagcaagc tgaacgtgat
     9661 ttacgtatgc tgaccagtcg cctggcatcc agctcattaa ataatacgcc gttcttgtcg
     9721 ccggaagaga catatcaact ttcgaaacgt ctgtggcagc aaattaccgt gcaaagtctg
     9781 gcggaaaaat ggcagcagtt aagaaagaac caggacgcat tttgggagca aatggtaaac
     9841 aatgaggttg ccgccaaaaa agcattgtct cctgcggcta tcctggcgct ggaaaaggag
     9901 tacgccaaca aaaagctggc ggcttacgtc ttcccaggca gaaatttatc gttaacagta
     9961 gacgcagacc cacaggcgga aattagcagc aaagaaacgc tggcggagaa tctgacatca
    10021 ttaacacttt ccaatggtgc cagggttatt ctggcaaaat ccgcgggtga agagcaaaag
    10081 ctacaaatta ttgccgtatc gaataaaggc gatttaagtt tccctgcgca gcaaaaatca
    10141 cttatcgcgc tggcaaataa agcagttagc ggaagcggcg ttggcgaact ctcctcttcc
    10201 agcctgaaac gctggagtgc ggaaaattcg gtaaccatga gcagtaaagt cagtggcatg
    10261 aatacgttgc tctccgttag cgcgcggact aataaccctg aacctggttt tcagttgatt
    10321 aaccagcgaa tcacccacag cacgattaac gataatattt gggcatcgct acaaaatgct
    10381 caaattcagg cgttgaaaac gctcgaccag cgtccagcgg agaaattcgc ccagcagatg
    10441 tatgagacgc gctatgctga tgaccgcacg aaattactgc aagaaaatca gattgcacag
    10501 tttactgccg cagatgcgct ggctgccgat cgccaattgt tttcatctcc agcggatatc
    10561 acgtttgtca ttgtcggtaa tgtcgcagaa gacaaactcg tggcgttaat tacgcgttac
    10621 ttaggatcaa tcaaacactc tgattcgcca ttagccgcag gtaaaccatt aactcgcgcg
    10681 acggacaacg catcggttac tgtaaaagaa caaaatgaac ctgtggcaca ggtttcacag
    10741 tggaagcgtt atgattcccg gacacctgtt aatctgccga cgcgtatggc gctcgatgct
    10801 tttaacgtcg cactggcaaa agatctacgc gttaatattc gtgaacaggc atctggggca
    10861 tacagcgttt cttctcgcct ctcggttgat cctcaggcca aagatatcag tcatttgctg
    10921 gcttttactt gtcaaccaga acgacatgat gaactgttaa cgttagcgaa tgaagtgatg
    10981 gttaagcgtc tggctaaagg gatcagtgag caagaactga atgaatacca gcaaaacgtt
    11041 cagcgcagcc tcgatatcca acagcgtagc gttcaacaat tagcgaacac cattgtaaat
    11101 agtcttattc aatatgacga tcctgcag
//
LOCUS       DAJOSY010000129        11090 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJOSY010000129 DAJOSY010000000
VERSION     DAJOSY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.129"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            167..1309
                     /locus_tag="OHN27_003768"
     CDS             167..1309
                     /locus_tag="OHN27_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2288135.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1316..2080)
                     /gene="glcC"
                     /locus_tag="OHN27_003769"
     CDS             complement(1316..2080)
                     /gene="glcC"
                     /locus_tag="OHN27_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCQ2288136.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2331..3830
                     /gene="glcD"
                     /locus_tag="OHN27_003770"
     CDS             2331..3830
                     /gene="glcD"
                     /locus_tag="OHN27_003770"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HCQ2288137.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3830..4882
                     /gene="glcE"
                     /locus_tag="OHN27_003771"
     CDS             3830..4882
                     /gene="glcE"
                     /locus_tag="OHN27_003771"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCQ2288138.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARVGTPLVTIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMVGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4893..6116
                     /gene="glcF"
                     /locus_tag="OHN27_003772"
     CDS             4893..6116
                     /gene="glcF"
                     /locus_tag="OHN27_003772"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HCQ2288139.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6121..6525
                     /locus_tag="OHN27_003773"
     CDS             6121..6525
                     /locus_tag="OHN27_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HCQ2288140.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6547..8718
                     /gene="glcB"
                     /locus_tag="OHN27_003774"
     CDS             6547..8718
                     /gene="glcB"
                     /locus_tag="OHN27_003774"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCQ2288141.1"
                     /translation="MSQTITQSRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9073..10755
                     /gene="glcA"
                     /locus_tag="OHN27_003775"
     CDS             9073..10755
                     /gene="glcA"
                     /locus_tag="OHN27_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HCQ2288142.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2601 a         2774 c         3077 g         2638 t
ORIGIN      
        1 caaaaggcat tgtggcgctt ctgtgtagtc tctgacctta tcgtaatcat taataaatac
       61 tatttattcg tcgtttgtat tcattgggtt gattattaag gtgccgttcg ttgctgttgc
      121 aacctgtttg tcaccgttaa ttatttgtta cccacagaac agaactatgg aatgcgatct
      181 gctgatgatt aaaattgaaa aagtaattaa taaaaatgac cttaaggcat ttatcgcttt
      241 tccgtcatca ctttatcccg acgatccaaa ctggataccc cctttattca ttgaacgcaa
      301 cgagcatttg tctgcgaaaa atccggggac ggaccatatc atctggcagg cgtgggtggc
      361 aaaaaaagcg gggcagatag ttgggcgtat taccgcgcaa atagatacct tgcaccgcga
      421 gcgttacggc aaagataccg gtcatttcgg catgattgac gccattgatg atccgcaggt
      481 ttttgccgcg ttgtttggcg cagcggaagc gtggttgaag tcacaaggtg caagtaagat
      541 cagcgggcct ttcagcctga atatcaatca ggaaagcgga ttactgattg aaggttttga
      601 cacaccaccc tgtgcgatga tgccacacgg caaaccgtgg tatgccgcgc atattgaaca
      661 actgggttat cacaaaggca ttgatttact ggcgtggtgg atgcagcgaa ccgatctcac
      721 tttctctccg gcgctaaaaa aactgatgga tcaggtgcgc aaaaaggtga ccattcgctg
      781 catcaatcgt cagcggtttg ccgaagagat gcagatcctg cgtgagattt tcaactctgg
      841 ctggcagcac aactggggat ttgtgccgtt taccgaacat gaattcgcga ccatgggcga
      901 tcaacttaaa tatctggtgc cggacgatat gatctatatc gctgagattg attctgcacc
      961 ctgcgcgttt attgtcggct tgccgaacat caacgaggcg attgccgatc tgaacggatc
     1021 gctctttccc ttcggctggg caaaattgct gtggcgtttg aaagtcagcg gtgtgcgaac
     1081 tgcgcgagta ccgctgatgg gcgtgcgcga cgagtatcag ttcagccgca tcggcccggt
     1141 gattgcgctg ttattgattg aagccttacg cgatccgttt gcccgccgga agatcgatgc
     1201 gctggagatg tcatggatcc tcgaaaccaa tacaggcatg aataacatgc tggaacgcat
     1261 tggggcggag ccgtacaagc gttaccgatt gtatgaaaag cagatttaat accagctaac
     1321 tcaggttcat ctccagcggc accgccgagc gaatcaaatg gtggccttcc agctcgatat
     1381 cgtggagatt ctttttcacg gtccgcacat gatcgcgtgc tgcgcgctgg gcgacgtgcg
     1441 gcagccgctg caacaccgcg ttgtagatcc gcgcatgctg gcgatcgatc tgcttttttt
     1501 gctgtggtcg atggtagaga ttatttacgc tggcaaacac tgaattaaac atcagatcgg
     1561 tcaatgattg cagcgtaaac accagcacct gattgtgaga agcctgacaa atggcgagat
     1621 ggaaagcgtg atccaactgc gcatgttcga tcagcgaaat ctctttgttg ttctcactgg
     1681 cggcgagcat tttttcataa cagcgggtta tcacaacaaa atcagcctgc gttcccagcg
     1741 ttgccgccag ccttgccgat tcgccctcca gtaatgcgcg aacgtcgagc agatcgtaca
     1801 gcgttcgcgg ctgcgtactg aacagatgga tcagcgggct ggtgtcctgc acccgattaa
     1861 gccgtgcgac acgagaatcg cgaccctgcg ccgtttcaat aatcccgcgc ccgcgcagca
     1921 cggtcagccc ttcacgcagt gcggagcgtg agaagccgag cttttcacac agtcgacgtt
     1981 ccgagggaag cggctgaccg accttcagta cgccgtcgat aattaaccgt tcgatactct
     2041 ctgcaaccac ttcgcaaata gggcgacgtt catctttcat tcccggacct cgtgcacagg
     2101 taggaccaat ttttctgttc ttaactcgca aaacacgcac atcacgtaag tgattatgtt
     2161 acatcaattt aacatttagt taaccaagac aaggtcacag agctggaaaa aaaatggtct
     2221 gaccggtagc taaagagata gacgaaaacg aaaagcccgc ttaataactg ttcacagaag
     2281 cagcgcgcaa aaatcagctg ccacacaaca caacaaagcg aagcctactc atgagcatct
     2341 tgtacgaaga gcgtcttgat ggcgctttac ccgatgtcga ccgcacatcg gtactgatgg
     2401 cactgcgtga gcatgtccct ggacttgaga tcctgcatac cgatgaggag atcattcctt
     2461 acgagtgtga cgggttgagc gcgtatcgca cgcgtccatt actggttgtt ctgcctaagc
     2521 aaatggaaca ggtgacagcg attctggctg tctgccatcg cctgcgtgta ccggtggtga
     2581 cccgtggtgc aggcaccggg ctttctggtg gcgcgctgcc gctggaaaaa ggtgtgttgt
     2641 tggtgatggc gcgctttaaa gagatcctcg acattaaccc cgttggtcgc cgcgcgcgcg
     2701 tgcagccagg cgtgcgtaac ctggcgatct cccaggccgt tgcaccgcat aatctctact
     2761 acgcaccgga cccttcctca caaatcgcct gttccattgg cggcaatgtg gctgaaaatg
     2821 ccggcggcgt ccactgcctg aaatatggtc tgaccgtaca taacctgctg aaaattgaag
     2881 tgcaaacgct ggacggcgag gcactgacgc tgggatcgga cgcgctggat tcacctggtt
     2941 ttgacctgct ggcgctgttc accggatcgg aaggtatgct cggcgtgacc accgaagtga
     3001 cggtaaaact gctgccgaag ccgcccgtgg cgcgggttct gttagccagc tttgactcgg
     3061 tagaaaaagc cggacttgcg gttggtgaca tcatcgccaa tggcattatc cccggcgggc
     3121 tggagatgat ggataacctg tcgatccgcg cggcggaaga ttttattcat gccggttatc
     3181 ccgtcgacgc cgaagcgatt ttgttatgcg agctggacgg cgtggagtct gacgtacagg
     3241 aagactgcga gcgggttaac gacatcttgt tgaaagcggg cgcgactgac gtccgtctgg
     3301 cacaggacga agcagagcgc gtacgtttct gggccggtcg caaaaatgcg ttcccggcgg
     3361 taggacgtat ctccccggat tactactgca tggatggcac catcccgcgt cgcgccctgc
     3421 ctggcgtact ggaaggcatt gcccgtttat cgcagcaata tgatttacgt gttgccaacg
     3481 tctttcatgc cggagatggc aacatgcacc cgttaatcct tttcgatgcc aacgaacccg
     3541 gtgaatttgc ccgcgcggaa gagctgggcg ggaagatcct cgaactctgc gttgaagttg
     3601 gcggcagcat cagtggcgaa catggcatcg ggcgagaaaa aatcaatcaa atgtgcgccc
     3661 agttcaacag cgatgaaatc acgaccttcc atgcggtcaa ggcggcgttt gaccccgatg
     3721 gtttgctgaa ccctgggaaa aacattccca cgctacaccg ctgtgctgaa tttggtgcca
     3781 tgcatgtgca tcacggtcat ttacctttcc ctgaactgga gcgtttctga tgctacgcga
     3841 gtgtgattac agccaggcgc tgctggagca ggtgaatcag gcgattagcg ataaaacgcc
     3901 gctggtgatt cagggcagca atagcaaagc ctttttaggt cgccctgtca ccgggcaaac
     3961 gctggatgtt cgttgtcatc gcggcattgt taattacgac ccgaccgagc tggtgataac
     4021 cgcgcgtgtc ggaacgccgc tggtgacaat tgaagcggcg ctggaaagcg cggggcaaat
     4081 gctcccctgt gagccgccgc attatggtga agaagccacc tggggcggga tggtcgcctg
     4141 cgggctggcg gggccgcgtc gcccgtggag cggttcggtc cgcgattttg tcctcggcac
     4201 gcgcatcatt accggcgctg gaaaacatct gcgttttggt ggcgaagtga tgaaaaacgt
     4261 tgccggatac gatctctcac ggttaatggt cggaagctac ggttgtcttg gcgtgctcac
     4321 tgaaatctca atgaaagtgt taccgcgacc gcgcgcctcc ctgagcctgc gtcgggaaat
     4381 cagcctgcaa gaagccatga gtgaaatcgc cgagtggcaa ctccagccat tacccattag
     4441 tggcttatgt tacttcgaca atgcgttgtg gatccgcctt gagggcggcg aaggatcggt
     4501 aaaagcagcg cgtgaactgc tgggtggcga agaggttgcc ggtcagttct ggcagcaatt
     4561 gcgtgaacaa caactgccgt tcttctcgtt accaggtacc ttatggcgca tttcattacc
     4621 cagtgatgcg ccgatgatgg atttacccgg cgagcaactg atcgactggg gcggggcgtt
     4681 acgctggctg aaatcgacag ccgaggacaa tcaaatccat cgcatcgccc gcaacgctgg
     4741 cggtcatgcg acccgcttta gtgccggaga tggtggcttt gccccgctat cggctccttt
     4801 attccgctat caccagcagc ttaaacagca gctcgaccct tgcggcgtgt ttaaccccgg
     4861 tcgcatgtac gcggaacttt gaggagcagg ctatgcaaac ccaattaact gaagagatgc
     4921 ggcagaacgc gcgcgcgctg gaagccgaca gcatcctgcg cgcctgtgtt cactgcggat
     4981 tttgtaccgc aacctgccca acctatcagc ttctgggcga tgaactggac gggccgcgcg
     5041 ggcgcatcta tctgattaaa caggtgctgg aaggcaacga agtcacgctt aaaacacagg
     5101 agcatctcga tcgctgcctc acttgccgta attgtgaaac cacctgtcct tctggtgtgc
     5161 gctatcacaa tttgctggat atcgggcgtg atattgtcga gcagaaagtg aaacgcccac
     5221 tgccggagcg aatactgcgc gaaggattgc gccaggtagt gccgcgtccg gcggtcttcc
     5281 gtgcgctgac gcaggtaggg ctggtgctgc gaccgttttt accggaacag gtcagagcaa
     5341 aactgcctgc tgaaacggtg aaagctaaac cgcgtccgcc gctgcgccat aagcgtcggg
     5401 ttttaatgtt ggaaggctgc gcccagccta cgctttcgcc caacaccaac gcggcaactg
     5461 cgcgagtgct ggatcgtctg gggatcagcg tcatgccagc taacgaagca ggctgttgtg
     5521 gcgcggtgga ctatcatctt aatgcgcagg agaaagggct ggcacgggcg cgcaataata
     5581 ttgatgcctg gtggcccgcg attgaagcag gtgccgaggc aattttgcaa accgccagcg
     5641 gctgcggcgc gtttgtcaaa gagtatgggc agatgctgaa aaacgatgcg ttatatgccg
     5701 ataaagcgcg tcaggtcagt gaactggcgg tcgatttagt cgaacttctg cgcgaggaac
     5761 cgctggaaaa actggcaatt cgcggcgata aaaagctggc cttccactgt ccgtgtaccc
     5821 tacaacatgc gcaaaagctg aacggcgaag tggaaaaagt gttgcttcgt cttggattta
     5881 ccttaacgga cgttcccgac agccatctgt gctgcggttc agcgggaaca tatgcgttaa
     5941 cgcatcccga tctggcacgc cagctgcggg ataacaaaat gaatgcgctg gaaagcggca
     6001 aaccggaaat gatcgtcacc gccaacattg gttgccagac gcatctggcg agcgccggtc
     6061 gtacctctgt gcgtcactgg attgaaattg tagaacaagc ccttgaaaag gaataacaaa
     6121 atgaaaacta aagtcattct tagccagcaa atggcgagtg caattattgc cgcaggtcag
     6181 gaagaggcgc agaaaaataa ctggtctgtt tccattgctg ttgccgatga cggcggtcat
     6241 ctgctggcgt taagtcgcat ggacgattgc gcgccgattg cggcttatat ctcccaggag
     6301 aaagcgcgta ccgccgcgct ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac
     6361 aacggacgta ccgcgttcgt gactgcgccg ttattaacgt cgctggaagg cggcgtaccg
     6421 gttgttgtgg atgggcaaat tattggtgcc gtgggcgttt ctggtttaac cggagcacag
     6481 gatgcgcagg tcgcgaaagc ggcagcagcg gtgttggcga aataagcgaa aacgaggaga
     6541 taaacaatga gtcaaaccat aacccagagc cgtttacgca ttgacgccaa ttttaaacgt
     6601 tttgtggatg aagaagtttt accgggaaca gggctggacg ctgcggcgtt ctggcgcaat
     6661 tttgatgaga tcgttcatga tctggcacca gaaaatcgtc agttgctggc agaacgcgat
     6721 cgcattcagg cagcgcttga tgagtggcat cgcagcaatc cggggccggt aaaagataaa
     6781 gcggcctata aatctttcct gcgtgaactg ggctacctgg tgccgcaacc ggagcgcgtg
     6841 acggtggaaa ccacgggcat tgacagcgaa atcaccagcc aggcggggcc gcagctggtg
     6901 gttccggcaa tgaacgcccg ctacgcgctg aacgcggcga acgctcgctg gggctcactg
     6961 tacgatgcgt tatacggcag cgacatcatc ccgcaggaag gggcgatggt cagcggctac
     7021 gatccgcaac gcggtgagca ggttatcgcc tgggttcggc gtttcctcga tgaatctcta
     7081 ccgctggaaa acggcagcta tcaggatgtg gtggcgttta aggtggttga taaacaatta
     7141 cgcatccagt tgaaaaatgg taaagaaacc acgttacgta ctccagcaca gtttgtcggt
     7201 taccgtggcg atgccgctgc gccgacctgc attttgctga aaaataacgg cctgcatatt
     7261 gagctgcaaa tcgatgccaa tgggcggata ggcaaagacg atccggcgca catcaacgat
     7321 gttatcgtcg aagctgctat cagtaccatt ctcgactgcg aagattcggt cgcggcggtt
     7381 gatgcggaag ataaaatcct gctgtaccgc aacctgctgg gcctgatgca ggggactctg
     7441 caagagaaaa tggagaaaaa cggtcggcaa atcgtgcgta aactgaatga cgatcgtcat
     7501 tacaccgccg ccgatggctc tgaaatttct ctgcacggac gctcgctgct gtttatccgc
     7561 aacgtgggtc atttgatgac cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg
     7621 gaaggcattc ttgatggcgt catgactggc gcgattgccc tctatgattt aaaagtgcag
     7681 aaaaactcgc gcactggcag cgtctatatt gtgaaaccga aaatgcacgg tccgcaggaa
     7741 gtggcgttcg ccaacaaact gtttacccgc attgagacaa tgctcggtat ggcaccgaat
     7801 accctgaaaa tgggcattat ggatgaagaa cgtcggacct cgctgaactt gcgtagctgt
     7861 atcgctcagg cgcgcaaccg cgtggcgttc atcaataccg gtttcctcga ccgtaccggc
     7921 gatgaaatgc attcggtgat ggaagctggc ccgatgctgc gtaaaaatca gatgaaatcg
     7981 acgccttgga tcaaagccta cgagcgtaat aacgtgcttt ccggtctgtt ctgtgggctg
     8041 cgcggtaaag cgcaaattgg taaaggcatg tgggcaatgc cggacctgat ggcagacatg
     8101 tacagccaga agggcgacca actgcgtgcc ggggcaaaca cagcctgggt tccgtcacca
     8161 accgctgcta cgctccatgc gctgcactac caccaaacca acgtacagag cgtacaagcc
     8221 aacattgccc agaccgagtt caatgctgaa tttgaaccgc tgctggacga tctgctgact
     8281 attccggttg ctgaaaacgc taactggtcg gcgcaagaga tccaacaaga gctggataac
     8341 aacgtgcagg ggattctggg gtacgtggtg cgctgggtgg agcaggggat tggttgttca
     8401 aaagtgccgg atattcacaa tgtggcgttg atggaagacc gcgcaacgct gcgtatctcc
     8461 agccagcata tcgccaactg gttacgtcac ggtattctga ccaaagaaca ggtgcaggcg
     8521 tcgctggaga atatggcgaa agtggttgat cagcaaaacg ctggcgatcc ggcttatcgt
     8581 ccgatggcgg ggaatttcgc taactcgtgt gcttttaaag ctgccagcga tttaatcttc
     8641 ctcggcgtga aacagccaaa cggctatacc gaaccgttat tacacgcctg gcgtttacgc
     8701 gaaaaagaaa gtcattaata gcgtcaaaaa agccccggca agttttattg tcggggctat
     8761 gatagtaact gcggtagtac atttatcccg gaaataaccg gagcgattat cagggtttaa
     8821 caatattaaa tacccgtagc gcattactag cgggcctaaa acaacaatat ggacatctta
     8881 acgatactct gcgtctgcgt gtgcggaaca gaaaccctta cccgatatac ttgttatgcc
     8941 agaggtagca attgcgttag cggcgtactg ttgctgtaaa gtataacgcc tatattttac
     9001 agttcagtcc tcttttgctt attacaacct taagtatatc aagcatataa agataataag
     9061 agactgaaca atatggttac ctggacccaa atgtatatgc cgatgggagg actggggcta
     9121 tccgctctgg tcgccctgat cccgataata ttcttcttcg ttgcactcgc ggtattacgt
     9181 ctgaaaggac atgtcgctgg agcaataacc cttatattat ctatcctgat tgcaatattc
     9241 gcctttaaaa tgccgattga tatggcattt gctgctgcgg gctatggctt tatttatgga
     9301 ttatggccaa tagcgtggat tattgtcgcg gcggtgttcc tgtataaatt aaccgttgcc
     9361 agcgggcagt tcgatattat ccgcagctcg gttatctcca tcaccgacga tcagcgtttg
     9421 caggtgttac tgattggttt ctcctttggt gcgttgctgg aaggagcggc tggctttggt
     9481 gcgccggtgg cgattaccgg tgcgctgctg gtgggcctgg gcttcaaacc gttatacgcg
     9541 gcggggctgt gtctgattgc caatactgcg ccggtggcgt ttggtgcgtt gggcgtgccg
     9601 attctggtcg ccggtcaggt aacgggaatc gatccgttcc acattggcgc aatggcggga
     9661 cgtcagttac cgttcctgtc ggttcttgtg ccgttctggc tggtagcaat gatggacggc
     9721 tggaaagggg tgaaagagac gtggccagcg gcgctggttg ctgggggaag cttcgctgtc
     9781 actcagttct ttacctctaa ctatattggt ccggaactgc cggatattac ttcggcgctg
     9841 gtgagtatcg tctcactcgc tttattcctt aaagtctggc ggccgaaaaa taccgaaacg
     9901 gcaatcagca tgggacaatc cgcaggtgcg atggtggtaa ataagccatc ttctggcggt
     9961 cccgtgcctt cagaatatag tctggggcaa atcattcgag cgtggtcacc gtttttaatc
    10021 ttaacggtgc tggtcaccat ctggaccatg aagccgttta aagcgttatt tgctccgggc
    10081 ggcgcgtttt attcactggt gattaatttc cagatccctc atttgcatca acaagtgttg
    10141 aaagcggcac ccattgtcgc ccaaccaacg ccaatggatg cggtgtttaa attcgacccc
    10201 ctctcggctg gcggcaccgc tatttttatt gcggcgatta tctctatctt catcctcggt
    10261 gtggggatca agaaaggtat tggcgtcttt gccgaaacgc taattagctt gaagtggccg
    10321 atactgtcga ttggcatggt gctggcgttc gccttcgtca ccaactattc tggcatgtcc
    10381 accacgctgg cgctggtact ggcaggtaca ggcgtgatgt tcccgttctt ctcaccgttt
    10441 ctcggctggc tgggcgtatt ccttaccggc tcggacacct cctctaacgc cctgtttggt
    10501 tcactgcaat cgaccacggc gcagcaaatc aacgtctctg acaccctgct ggtggcagca
    10561 aacaccagcg gcggcgtaac tggcaagatg atctccccgc aatctatcgc cgtggcctgc
    10621 gccgcgacgg gcatggtggg ccgagaatct gaactgttcc gctacaccgt gaagcacagt
    10681 ctgatttttg ccagcgttat cggcattatc accctgctgc aggcgtatgt gtttaccggg
    10741 atgttagtct cgtaaatcaa taaagccgga tgatcgttaa tatcatccgg cttcatttac
    10801 attattctgt cttattaatt gaaaatcatt tctcgcttta ttatccatgc agcaataatg
    10861 ttttgcgttc agataacctg ctaaataatt aagagcgtga gaacatctat ttttttatca
    10921 cgcaaaacaa gtctgtatta attatgttcg cttgatgagc aaacaccgtt ctatctgttg
    10981 cttttgcaat cttaatcgtt aaaactacca acaaatttat atagttacgt tgtgaaatgt
    11041 tgttaatttt ttgtttgttg aaatttcgta aaaatagcca gatagacatc
//
LOCUS       DAJOSY010000130        10770 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJOSY010000130 DAJOSY010000000
VERSION     DAJOSY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.130"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(53..511)
                     /locus_tag="OHN27_003776"
     CDS             complement(53..511)
                     /locus_tag="OHN27_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCQ2288143.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(566..1417)
                     /gene="manZ"
                     /locus_tag="OHN27_003777"
     CDS             complement(566..1417)
                     /gene="manZ"
                     /locus_tag="OHN27_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCQ2288144.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1430..2230)
                     /gene="manY"
                     /locus_tag="OHN27_003778"
     CDS             complement(1430..2230)
                     /gene="manY"
                     /locus_tag="OHN27_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCQ2288145.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2293..3264)
                     /gene="manX"
                     /locus_tag="OHN27_003779"
     CDS             complement(2293..3264)
                     /gene="manX"
                     /locus_tag="OHN27_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCQ2288146.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3727..5283
                     /gene="yoaE"
                     /locus_tag="OHN27_003780"
     CDS             3727..5283
                     /gene="yoaE"
                     /locus_tag="OHN27_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCQ2288147.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(5287..6885)
                     /locus_tag="OHN27_003781"
     CDS             complement(5287..6885)
                     /locus_tag="OHN27_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCQ2288148.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(7016..8380)
                     /gene="sdaA"
                     /locus_tag="OHN27_003782"
     CDS             complement(7016..8380)
                     /gene="sdaA"
                     /locus_tag="OHN27_003782"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCQ2288149.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8564..9142)
                     /locus_tag="OHN27_003783"
     CDS             complement(8564..9142)
                     /locus_tag="OHN27_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCQ2288150.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTACIIRELALQIGVKP"
     gene            complement(9146..10507)
                     /gene="pabB"
                     /locus_tag="OHN27_003784"
     CDS             complement(9146..10507)
                     /gene="pabB"
                     /locus_tag="OHN27_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCQ2288151.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10581..10760
                     /locus_tag="OHN27_003785"
     CDS             10581..10760
                     /locus_tag="OHN27_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCQ2288152.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         2693 a         2736 c         2749 g         2592 t
ORIGIN      
        1 tatgtctgat tattaaaaat acactaaaca tagctttggc taaattcata acttattgag
       61 ttgaaacgag aagcttataa attttttcca gatcgtcgat atttcgaacg cggattaaca
      121 gccgacgctg ttctaattgc atcaccaaca cgccatcttc cgacaagttc atcgctttga
      181 ttcggctata ttcaatccag acattggcga agaaaaaacc tttttgttta aagatgatct
      241 tcggaacgcg gatccagaat atataaaaac ccatcagagc cagtgcgctt aataaccatg
      301 tcgttattaa cgcaccatga ttcgtgacgt tgttatagat aagaatgaca atcaatccga
      361 cgaagataac gctatcgatg cgaccacgcc ggagcaaagg aattgccagc agggtggggc
      421 cattacggcg gggcatgatg aactgatcgt agatcgcgaa ggccaggagt gcggcgatga
      481 aaagaatcag taccaggtcc gtgattgtca ttaatcctcc agataaaaaa acggggccaa
      541 aaggccccgg tagtgtacaa cagtcttaca gtcccagcag gccgcaagcg taaccagcga
      601 taccgatgac gaagaagcca acgatgatcc acagcgggtt aacttttttg cgcagtagcc
      661 acatacaagc aaaggtcagc agcagtggta ccaggcctgg cattaactgg tccagaatag
      721 tctggacagt agtaacgtgt tctttgcccg tctggtcagt aatgcgagag acaaccagcg
      781 ggatgttgac atgtgtccac ttgttaacca atgcccccat gacaaacagg ccgaggatag
      841 acgccccttc cgtcagtttt tgcaggaagc caccacccat atctttaacg atatcgatac
      901 ctttggagta accatacgct acgccgtagt aacgggttgc cagacgcacc aggttaaaca
      961 ggatgaagaa cagcagcgga cctaacaggc tgccgctcat cgcgataccg gcacccagtg
     1021 ctgcaaatac cggacgtacg gttccccaga agatcgggtc gcctacacca gccagtggcc
     1081 ccatcaaacc gactttgata ccgttgatag caccgtcgtc gatctctgcg ccattagcac
     1141 gctgttcttc cagcgccagg gttacgccga gaatcggcgc agccacgaac ggctgggtgt
     1201 taaagaactc caggtgacgg cgaatagctt gtttacgagc ttcgttgttc tcagggtaga
     1261 ggcgacgaat tgccggtacc atagagaagc agaaacccag tgcctgcata cgttcgaagt
     1321 tccatgaacc ctggaagagg ttagaacgca ggaagacgcc acgaatatca ctttgagtga
     1381 gttttttctc ggtggtagtt tgagttgtat caaccatttc gctcacctgt tagtccagtt
     1441 cgttatcgag atcgttgtta ccagctgcct gagcaggcgc accggctacg cggttgtatt
     1501 tcgggctaag ttggatgtag agcactgcca taacagtacc aatcacaccc agagcaacca
     1561 ggttaaagtt ggtgaatgct gcggttacga agccgaggta gaagaacggc atcaggtagc
     1621 cagcacgcat catgttgata accatcgcat aaccaaccac cacgatcatg ccaccggcga
     1681 tattcagacc attggtcacc acttccggaa tcgcattcag catgttctgt acttcgctgg
     1741 taccaacaga cagcgcaacg atgacggccg gaatagccac acgcattgct tgcaggaaca
     1801 gagaagaaac gtggatccag gaaatcgctg tcaggttgcc gttatcagca gccttatccg
     1861 cagcgtgctg gaaagcaacg gtaatagtac gaacgatgat ggtcagtacc tggcccgcag
     1921 cggccagagg gattgccagt gcgatacctg caccaatgct ctgatgacct gcgataacca
     1981 gaatggtaga aatgatagaa gccagagcgg cgtcaggcgc aactgcagca ccgatgttca
     2041 tccagcccag cgcgatcatt tccagcgtac caccgataat aataccggtt ttcatatccc
     2101 caagaacgat acccaccagg gtacacgcga ttagcggacg gtgaaactga aattcatcga
     2161 ggattgatcc catacctgcg atacaggcta cgataaatac cagcacaatt tgaagagtgg
     2221 taatctccat tgtacttctc ctgtttacgt aagacttaag tgtgaaaact gagtgataat
     2281 caacacaata cgttacttat cgattttgct gatcagatcc atcattttca gtttcggatc
     2341 ggtggaaacc ttacggactt ccagctcaat accgcgcgca ttcagtttct tgaacgcctc
     2401 gatatctttt tcatcaaccg aaaccgcgtt attcacctgg gttttaccct gacggaatgc
     2461 cataccaccg acgttaacag aggtgatttt cacgccgcct tcaacgagac gctctacatc
     2521 tgttgggttg gtaaataaca gcattacgcg ttcgccagca tatttcgggt tgttgtagac
     2581 gcgaatcatt ttggcaacat caactacgtg tgctgttacg cccggaggtg caacctgggt
     2641 gagcagtgtc ttacgaacgg tatccgcagc cacttcatca ctaacaacaa taatacggga
     2701 gacattggtt tctttggtcc agcgggtggc gacctgaccg tgaatcagac ggtcgtcgat
     2761 acgcgcaagg ccaataacca tgtagtcgtt tggccccatt ggttttgccg gagttggagc
     2821 cgcttttggt gctgcggcag cgggtgctgg cgcggctttt tcaaccggtt tggctttcag
     2881 tgctttcacg ccttcacggc ctgtttctac tgccagcgca accagttcat caaagcttgg
     2941 gtcatcatca cgagccatta acgtttccac gagcattgga atgttaacgc ctgcaatgac
     3001 ttcataatgc tctttgtcga cgacaatgcg gctggcagca ttgaacgggc tgcctcccca
     3061 tgtatcaacg agaaacagca cgcctttagt ggtgtcgagt tttgccaact gagcgttgta
     3121 cttttcaatc agcgtttcgg cattttcacc tggaacgaaa tcgatccagc cgacgttttc
     3181 ctgctcgcct aacagcattt ctgccgtttt aagcaactgc tctgcagccc aaccatgtgt
     3241 gcctataaca atagcaatgg tcacttgcta cctcctttat tatcgttaac acctcaacgt
     3301 gccagatgta tttttgaatc gtcgtccaca atcgaatcga ttcagataag ggctacaacc
     3361 aaaaacccct gcgtttcgcg atttatttta gatatcgaaa aaattatttt atgtgatgaa
     3421 gatccgtaat ttaactttcg attagcagaa atttcgaaag gtaaaatatc cttgtcacat
     3481 tcgtttgcaa aggaaggtaa atctttgcca aatcagaggc gtctctgata tgtttaactc
     3541 ccgtttaaca accatggagt atagggcagt agcccgcagt atggatcgtc accgacgtca
     3601 tttcagcatc aggcctttta acgcctgcct ttctggcact ctatgccgca cctttcgttt
     3661 gcattttgtc gttacgcctg cattatttct ggcgtcgaat agctattcct taagcaggag
     3721 cttgtcatgg aattcttaat ggacccctca atttgggcgg ggctactcac gcttgttgtt
     3781 ctcgaaattg tgctgggtat cgataacctg gtcttcatcg ccattcttgc tgacaaactg
     3841 ccgccaaaac aacgcgataa agcgcgtttg ctggggttat cactggcgct gattatgcgt
     3901 ctggggctgc tgtcgctgat ttcatggatg gtcacgctga ccaaaccgct atttaccgtc
     3961 atggatttct ccttctccgg acgcgacctg attatgttgt tcggggggat attcttgctg
     4021 ttcaaagcaa caaccgaact gcatgaacgg ctggaaaacc gcgatcatga ttccggccac
     4081 ggtaaaggct acgccagttt ctgggtggtc gtcacacaga ttgtcatcct tgacgccgtc
     4141 ttctcgttgg atgcggtaat tactgcagta gggatggtta accatctgcc ggtgatgatg
     4201 gcggcggtag tgattgcgat ggcggttatg ttgctggcat ccaaaccgct gacgcgattc
     4261 gttaaccagc accccacggt ggtggtgctc tgtctgagct tcctgttaat gattggtctg
     4321 agtctggtgg cagaaggttt cggtttccac attccgaaag gttacctgta tgccgcgatt
     4381 ggcttctcga tcatcatcga agtgtttaac cagattgcgc gtcgcaactt tattcgccac
     4441 cagtcgactt tgccgctgcg agcgcgtact gccgatgcca tcctgcgttt gatgggcggg
     4501 aaacgtcagg ccaatgttca gcacgatgcc gataacccga tgccgatgcc gatcccggaa
     4561 ggtgcatttg ccgaagaaga acgttacatg attaacggcg tactgacgct ggcgtcgcgt
     4621 tctctgcgcg ggatcatgac gccgcgcggt gaaataagct gggttgacgc taatctcggg
     4681 gtcgatgaaa tccgcgagca actgctctct tcaccgcaca gtctgttccc ggtatgtcgc
     4741 ggtgaactgg atgaaatcat cggtattgta cgtgctaaag aactgctggt ggcgctggaa
     4801 gagggcgttg atgtggcggc gattgcttcg gcgtctccgg cgattatcgt cccggaaacc
     4861 ctcgatccga tcaacctgtt gggcgtgctg cgtcgtgctc gcgggagctt tgttatcgtg
     4921 accaacgagt ttggtgtggt acaaggtctg gtcacgccgc tggatgtgct ggaagccatt
     4981 gcgggtgaat tcccggacgc tgacgaaacg ccggaaatca ttactgatgg tgacggctgg
     5041 ctggtaaaag gcggtacaga tttgcatgcc ttgcagcagg cgcttgatgt tgagcacctt
     5101 gccgatgacg atgatatcgc gacggtcgcg ggcctcgtga tctcggcaaa tggtcacatt
     5161 ccccgtgtgg gcgatgtgat tgatgtaggg ccactgcata tcaccatcat tgaagccaat
     5221 gattatcgtg ttgatctggt tcgcattgtt aaagagcaac cggcgcacga tgaagatgag
     5281 taagcattaa cgtaacggca taatgggcgt gatatgtcca ttatgccggg cgggcggcgg
     5341 ttggctgccc gccagccatt tgggaaaatc acgtagcggc atcggctgtg cgtacaaata
     5401 cccttgcaaa atatgcacgc catgacggcg caaatacttc gcctgttctt gcgtttccac
     5461 gccctccgcc accagttcaa tattcagtct ttgccccagc gcgatgatga tatcggtcac
     5521 cgtcgagtta accgcgtcag aacctatagc tgcggtaaat gacttatcaa ttttcagcac
     5581 gtcaggacgt aatgtttcaa gccaggaaaa tgaactgttg ccggtgccga agtcatcaat
     5641 cgccagtttg acgtttttac gatgtagctc gcgggcaatc cgataatcaa catccagtaa
     5701 ggcatcgcgt tcggtgattt cgaggatcag ttgctgaatc gggtgagcgc taaaccagta
     5761 ctgattgaga tcttttatca gcacaccacg gcgaaaatgg ctgggtgcga cgttaatgcc
     5821 aacatgaaac tgactactca tcgggaaaac atggcgctga cgaatggttt ctgccatcac
     5881 atagcgggtc agtggcacaa ttaaatgatg ttcttccgcg ataggaataa acacatccgg
     5941 tgaaatccag ccctgacgcg gattgttcca gcgcagcaaa atctctacac caatacattg
     6001 ctggctgcgc gcattaagca aaggctgaca gaacaattcg aactcatgtt gcgccaggcc
     6061 cagattgatt tcgcgggaaa agctcatccg gtaagcggtc gccagccagg cgatgtagcc
     6121 caccagtaaa cttagcagca ccgccagcgg taattgtgtt ggaagatagt gccatgccag
     6181 cgccgtagcc cccggaccat taacgttaat ggtaaaagga aagcgttgcg aagaaacctg
     6241 atagttttca ttgtcttcag gttgcggaag ggaatctacc agcccattac catagagcaa
     6301 atgccgtttg tccaccgtca ggctggcgct actgatttgc ggcagttgtg gctcaaggag
     6361 cattgccgtc agtaagtcga tgttaatcat ctccatgacc ccagcattgc tactgcccgc
     6421 tgcaggcgtc cattgaatca aaaccggact gcctttaatc agggcacggt cgatcgtcag
     6481 gcgtaaaagt ggttgcggtg caggaagttc agccagaatg tccacgacgg gcacattgcg
     6541 ataaccaaaa atgctggagc aataaagtgt gccgtcttgc accaggccaa tggatcgcac
     6601 agtttggagt tttgccgcct gtttacgtaa tggcaaatgg gcgacagagc agggcagacc
     6661 aatcagcgga agcaagactt cgctacctgc ctgtagggga agcagtactt tatctaattc
     6721 ctctacagcg tgattggcga attgtactac ccgttgttga tttaagttac gctgtgaaat
     6781 aaatcgcacg ctcagggtcg aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat
     6841 tcgattacgg cgataggttt taatgatccg ttgtgctttt tgcatgcggg ccaccctgta
     6901 agcgtatggc gacaaacgcc tgacgcaaca gtggaagtgt agtggggaat attacagcag
     6961 acgagaaagc gggtataaat tcgcccatcc gttgcagatg ggcgagtaag aagtattagt
     7021 cacactggac tttgattgcc agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt
     7081 ccttaccggt ttcgtacatc gtttcgatga ccttatccag cgagacgcgc ggtgcactgg
     7141 tgcggcgcag agccatccgt gcggcgttaa tcgccttcac agaggcaatg gcattacgct
     7201 caatgcacgg cacctgaacc tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt
     7261 ccatgccaat ttccgccgcc acgcaaacct gttccgggct accgcccagc agttctgcaa
     7321 gacccgcagc agccattgaa caggcaacac ccacttcgcc ctggcaacca acttccgcac
     7381 cggaaataga ggcgttcatt ttatacaatg caccaatcgc gcccgctgcc ataaagtaac
     7441 gggtatagat gtccgggctg accgattcaa taaagtggtc atagtaagcc agcactgccg
     7501 gaacgatacc gcaggcaccg ttggttggcg cagttaccac acgaccaccg gcggcgtttt
     7561 cttcgttaac tgccagcgca aacatgttta cccagtcaat gacattcatc ggatcgttag
     7621 acagtttatc gctggaaact aacatccggc gcagggcaga cgcacgacgt ggcacgcgca
     7681 gcgggcctgg cagtacacct tcggtgttca tcccgcgatc gatacatgcc tgcatggttt
     7741 gccagacatg cgcgaaatac tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct
     7801 gcatagcgag accagacagc gaatagccgg tttcattaca gtacgcgagc agttcggtgg
     7861 cagatttgaa cggatacggc acgctcactt cgttggcagc atcctgacca aagtgttctt
     7921 catcgacgat aaaaccaccg ccgatggaat aataagtttt gctgtagacg acttcatcgc
     7981 cgttataggc gtggatttgc ataccgtttt catgcagcgg caggttgccg ttatgaaaac
     8041 gcatcccgtt gtcgcgcggg aaatccactt catgccgtcc ctgtgccagc agcagacgtt
     8101 cgcgctcttc tacgtcgcga ataaaaccgg gaatactgtc gatatccacg gtggcaggtt
     8161 cgttacctgc aagacccata ataatggcga tatcggtgtg gtggccttta cccgtcagcg
     8221 acagtgaacc ataaacgtcc acggcaacgc gagtaacgct atccagtaag cctttttcga
     8281 ccagatcatc gacgaactgt ttacccgcct tcataggccc tacggtatgg gaagatgagg
     8341 gaccaatccc caccttaaac atgtcgaata gactaatcac gataatactc ctgacaaggt
     8401 gactggactt ccagtaacga actaataaca gcgcatagtg taagagggaa cgcggcgact
     8461 ggcttaacta ttcacatgaa ttaaactaat caaaaaccgc gatttaacta atattgcgat
     8521 gattgtctca agtagtgttt tgtagatgta aagataagta tagtcagggt ttcacaccaa
     8581 tttgcagcgc cagctcacga attatgcatg cggtcattcc ccatacaaaa tactgttcgt
     8641 accaggacag ccatacccga tgtgaatcgc cacggcggta gatatctaaa gggtgataac
     8701 gacccagatg taatgcctgg gcgagcggca tttcaaacac cgccgagact tcatcttcac
     8761 tggcgcgata cggcagatcg ggcgggataa tgccgaccac tggggttacc tgatagccag
     8821 ttacgctatc gacgggcggc agcacgccga taacttcaac ggcggaaggc ggtatagcga
     8881 cctcttcttc agcttcgcgc agcgcggcgg cgataactga tgcgtccgtg tcatcgactg
     8941 cacctccagg gaatgccact tgtccagcgt gtttacgcag atgaattgaa cgctgagtca
     9001 gcaacaaccc cggttgcggt cgacggacga tggggattaa cacggcagcc tgacgatgat
     9061 ttagggtttc ccggttaatt tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa
     9121 gcgtcaggct acggtattcc acgtcttact tctccagttg gcgtaatatc ttattaactt
     9181 tatcaaaagt ttcctgatat tccgcttctt cctggctatc ggcgacaatt ccaccgcccg
     9241 cagagcagta tatttgtccg ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca
     9301 tgttgccgca aaagctcaaa tagccaatgc tgccgcacca ggcattacgt cgctgcggtt
     9361 ccagttcgtc gataatttcc atagcccgta ctttcggagc cccggttatt gagccaccag
     9421 gaaaagctgc gcgcagcaga tcgctggcgt gtaactgttc tggtagtcgc gccgttatag
     9481 tgctgaccag atgatgcacg gcagggaagg gttccaccac gaacagctct ggtactttta
     9541 ccgaaccggc tacggcaaca cgaccgatat cattacgcat taaatcgaca atcatcagat
     9601 tttcggcacg atctttcgct gagttcgcca gtttttctgc ttgtttgcta tcttcctgag
     9661 gatcgggcag gcgtggtagc gtgcctttaa tcgggcgggt ctggatttca ctattatcac
     9721 aaagaataaa ccgctctggc gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag
     9781 cgctaaatgg cgcgcggttg gcctgattaa gctgaaggaa tgcctgccat tcatcgccag
     9841 aataggtcgc atgaaaacgc tgggcgagat tcacctgata gcaatcaccg ctgtgcagat
     9901 attcctgtac ctggcgaaat ttttcgccgt actgctcgcg ggtcatattg gattgccagt
     9961 cggaagtgag cgtgaaatct tcctgcggcg agaattgctg gctttccagc caggcccgac
    10021 gggcattgac atcattatga ctcagcaaag aaactgtatg acgctggtgg tcgacaatga
    10081 gcgcccaatc gtagataccc actgccatat ccggcagaac gatatcttgt tccgcaattt
    10141 ctggcagtga ctcaaaacgg cggcccagat cgtagccaaa caaccccagt gcgccgccct
    10201 gaaatggcaa atcttcgtta tgcgttgggc gaatgtctgc gcgatccagc acctgctgga
    10261 gcacctgtag cgggtcatca gtggtcgttg tgcgtttttc gctttcacta acaacggttt
    10321 ctttaccgaa agtggttaaa gtgcaaatcg gatcggcgac cacaatatca aagcggctat
    10381 acggatgatc ggcatagccg gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg
    10441 agaaataaaa ttcagcggcg tcctgacgcc agggtaaagt aatcacagcg ggagataacg
    10501 tcttcatcag tcctgactct actggctatg tgccgggtag catgaaataa ttagcgccaa
    10561 caatttagca ggagttaaca atgtttgcag gtttaccttc actcacccat gaacagcagc
    10621 aaaaagctgt cgagcggatc caggaactga tggcacaagg gatgagcagc ggccaggcaa
    10681 tcgcgctggt ggcagaagag ctgcgtgcca accactccgg cgagcgaatc gtggcgcgtt
    10741 ttgaggatga agacgagtaa cacactaatc
//
LOCUS       DAJOSY010000131        10638 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJOSY010000131 DAJOSY010000000
VERSION     DAJOSY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.131"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..2179
                     /locus_tag="OHN27_003786"
                     /pseudo
     CDS             1..2179
                     /locus_tag="OHN27_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2515..3492
                     /gene="csiD"
                     /locus_tag="OHN27_003787"
     CDS             2515..3492
                     /gene="csiD"
                     /locus_tag="OHN27_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCQ2288153.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3511..4779
                     /gene="lhgO"
                     /locus_tag="OHN27_003788"
     CDS             3511..4779
                     /gene="lhgO"
                     /locus_tag="OHN27_003788"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCQ2288154.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYHKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4802..6250
                     /gene="gabD"
                     /locus_tag="OHN27_003789"
     CDS             4802..6250
                     /gene="gabD"
                     /locus_tag="OHN27_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCQ2288155.1"
                     /translation="MKLNDSKLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLLMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6264..7544
                     /gene="gabT"
                     /locus_tag="OHN27_003790"
     CDS             6264..7544
                     /gene="gabT"
                     /locus_tag="OHN27_003790"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCQ2288156.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPDGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAGIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7855..9255
                     /gene="gabP"
                     /locus_tag="OHN27_003791"
     CDS             7855..9255
                     /gene="gabP"
                     /locus_tag="OHN27_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCQ2288157.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9276..9938
                     /gene="csiR"
                     /locus_tag="OHN27_003792"
     CDS             9276..9938
                     /gene="csiR"
                     /locus_tag="OHN27_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCQ2288158.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9939..10388)
                     /gene="kbp"
                     /locus_tag="OHN27_003793"
     CDS             complement(9939..10388)
                     /gene="kbp"
                     /locus_tag="OHN27_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCQ2288159.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10472..10630)
                     /locus_tag="OHN27_003794"
     CDS             complement(10472..10630)
                     /locus_tag="OHN27_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCQ2288160.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2673 a         2622 c         2775 g         2568 t
ORIGIN      
        1 cggggatcta aattccaaat taaatacatt agaatatcta aactgtataa caaatattat
       61 taattcttgt ggagtttacc ctcaaggatt aaaagacaga gaaattatat caacttttca
      121 cgcagaaaaa gttattaatg atctgttaaa aaacgattat aaaatttccc tttctccaga
      181 tacaacttat cgagagttga ataaagcagc acagcgtagc attacagcgc cagacaggat
      241 aggagaagga aaaacatggg tttatcaacg agatacaatg attgaaagag gtgataacag
      301 cggtgtttat cagtatggtc ctgctgaaca cttcacccac attatatctg acaaaccttc
      361 cccaaaagat aaatatgttg catatgctat taacattcct gactatgagc tggcagccga
      421 tgtatataat attaacgtga cgtcaccttc cggacagcaa gaaacattta aaatattaat
      481 caatccagaa catctacggc aaacacttga gcgtaaatct cttactgctg ttcagaaatc
      541 acaatgtgaa atcatcaccc ccaaaaaacc tggcgaagcg attcttcatg cttttaatgc
      601 cacctaccag cagatcagag aaaatatgtc tgaatttgca cgttgccatt atgggtatat
      661 acaaatccct ccagtgacaa ctttccgtgc cgacggacca gaaactcccg aagaagaaaa
      721 aggttactgg tttcacgctt atcaacccga agatctttgt accatccaca atccaatggg
      781 agatttgcag gattttatcg cattggttaa agatgctaaa aaatttggta tcgatatcat
      841 tcctgattat acctttaact ttatgggaat cgggggtagt ggtaaaaatg acctggatta
      901 tccctctgct gatatacgag cgaagatcag taaagatata gaaagtggta tccctggcta
      961 ttggcaaggt caggttttga ttccatttac tatagatcca gtaacaaaag aacgtaaaca
     1021 aatccatcca gaagatatac atctcactgc aaaagacttc gaagcaagta aagataacat
     1081 ctctaaggat gaatgggaaa acctccatgc attaaaagaa aagcgtttaa atggaatgcc
     1141 taaaacaaca cccaaaagtg accaggttat tatgttgcaa aatcaatacg ttcgtgaaat
     1201 gcgaaaatat ggcgtacgag gtttacgtta tgatgcggca aaacactcaa aacatgaaca
     1261 aatagaaaga tcaataaccc caccgcttaa aaattataat gagcggttac acaatactaa
     1321 cttatttaac ccaaaatatc ataaaaaagc cgttatgaat tacatggaat atctggtaac
     1381 ttgtcagttg gatgaacaac aaatgtcatc actgctttat gaaagagatg atttaagcgc
     1441 cattgatttt tcattgctca tgaaaacgat aaaagccttt tcatttggtg gagatctcca
     1501 aacccttgca tcaaaaccgg gttccacaat ctcaagtatc ccatcagaaa gacggatatt
     1561 gattaacatt aaccacgatt ttcctaacaa tggtaatctt ttcaatgact ttctatttaa
     1621 ccatcaacaa gatgaacaat tagcaatggc atatatagcc gctctgccgt tcagcaggcc
     1681 tttagtttac tgggatggcc aagtattaaa atcaacgact gaaattaaaa attatgatgg
     1741 gtccacgcgt gtcggcggtg aggcgtagct taataaaggt tgctctacct atcagcagct
     1801 ctacaatgaa ttccacgcat tatatataga taaagcagga atatggagcg catttgaggg
     1861 tgtatttgca actaaaaacg ttctggcctt tagtcgtggg gattctgtga acattaatca
     1921 ctctcctcat gatggactag ttataataaa taaaggaaac gaagaagttg aaggtacctg
     1981 gcctaacaaa ttgcaacctg gaatatacaa aaacatgggg agtaatagcg ttaacattat
     2041 tattaataat acccgaaaaa ttatcccccc tggtaaagta tttacgctta gaggcggaac
     2101 tctaaatatc aatattcctg ggcgtagcgc tcttctttta gggaaaacag gagaaccgcc
     2161 gaactatctc tatttataat ttaatgttat atctgccccg ataaaacggg gcagataata
     2221 tgtttagttt actaacggtc attttgcagt gaagccattt actgtttttt atcgaccaga
     2281 taatctgttc tctaatgtta actcccccta acctgttgct ttagttattc atttcctgtc
     2341 tcactttgcc ttaataccct acgttaaatg ttactaattt gttgcttttg atcacaataa
     2401 gaaaacaata tgtcgctttt gtgcgcattt ttcagaaatg tagatatttt tagattatgg
     2461 ctacgaaatg agcatcgcca tgtcacccta catctcataa gaggatcgct tctgatgaat
     2521 gcactgaccg ccgtacacaa taacgctgtc gattcaggcc aggactatag cggattcact
     2581 ctcatcccgt cggcgcaatc cccgcgtctg ctggaactca ccttcaccga acagacgacc
     2641 aaacagtttc tcgaacaggt tgccgaatgg cccgtgcagg cgctggagta caaatcgttt
     2701 ctgcgttttc gggtaggcaa aattcttgac gatctgtgtg cgaatcagct gcaaccattg
     2761 ctgttgaaga ccctgctaaa ccgtgctgaa ggtgcgctgt tgatcaatgc ggtgggtatc
     2821 gatgatgtgg cgcaggcaga tgagatggtg aagctggcaa cggcggtagc gcatctgatt
     2881 ggtcgctcaa atttcgatgc gatgagcggt cagtattacg cgcgtttcgt ggtgaaaaat
     2941 gtcgataact cagacagcta tctgcgtcag ccgcaccgcg tgatggaact gcacaacgac
     3001 ggcacgtatg tcgaagagat caccgattac gtgctgatga tgaaaatcga tgagcaaaac
     3061 atgcagggtg gaaattcgtt gctgctgcat ctcgatgact gggaacatct ggaccactat
     3121 ttccgccacc cgctggcgcg tcgcccaatg cgctttgccg cgccgccgag taaaaacgtc
     3181 agcaaagatg ttttccatcc ggtgttcgac gttgatcaac agggtcgccc ggtgatgcgt
     3241 tatatcgacc agttcgtcca gccaaaagac ttcgaagaag gcgtgtggtt gagcgagctt
     3301 tcggacgcca ttgaaaccag caaaggcatt ctttctgtgc ccgttcccgt tggcaaattc
     3361 ctgttgatta acaacctgtt ctggctgcac ggtcgcgacc gctttactcc gcacccggat
     3421 ctgcgccgtg aactgatgcg tcagcgtggc tatttcgctt acgccactca ccactaccag
     3481 acgcatcagt agcgcaaagg aattgagcgg atgtatgatt ttgtgattat tggcggtggc
     3541 atcatcggca tgtcgaccgc catgcaactg attgatgtct acccggacgc ccgcattgcg
     3601 ttgctggaaa aagagtccgg cccggcctgt caccagacgg gccacaacag cggcgtgatc
     3661 catgccgggg tttattacac gcccggtagc ctgaaggcac agttttgcct ggcgggaaac
     3721 cgcgccacta aagccttttg cgatcaaaac ggcattcgct atgacaactg cggcaagatg
     3781 ctggtcgcca cctctgaact cgaaatggaa cggatgcgcg cgttgtggga acgcacggcg
     3841 gcaaacggta tcgagcgcga gtggttaaac gccgatgaac tgcgcgagcg cgaaccgaat
     3901 atcaccgggc ttggcggtat ttttgtgccg tccagcggca ttgtcagcta ccgcgaagta
     3961 acggcggcga tggcaaaaat tttccaggcc agaggcggcg agattatcta taacgccgaa
     4021 gtcagcggcc tcagtgagca taaaaacggc gtggtgatac gtacccgcca gggcggcgaa
     4081 tatgaagcat cgacgctgat tagctgttcc gggctgatgg ctgaccggct ggtgaaaatg
     4141 ctcggactcg aaccgggctt tatcatctgc ccgttccgtg gtgagtattt ccgccttgcg
     4201 ccggagcata accagattgt taaccacctg atttacccca ttcccgaccc cgcgatgcca
     4261 tttttgggcg tgcatctcac ccgaatgatc gatggcagcg tgaccgtcgg gccaaacgcg
     4321 gtgctggctt ttaaacgcga aggctaccac aagcgcgact tctcgtttag cgacacgctg
     4381 gaaattttgg gctcgtcggg gattcgccgg gtgctgcaaa accatctacg ctcaggactg
     4441 ggcgagatga aaaactcgct gtgcaaaagc ggctatctgc ggctggtgca aaagtattgt
     4501 ccccggcttt cgttaagcga tctccagccc tggcccgccg gtgtgcgggc gcaggcggta
     4561 tcgccggacg gcaagctgat tgacgatttt ctgtttgtca ccaccccgcg cacgatccac
     4621 acctgcaatg cgccctcccc ggcagcgaca tcagcaattc ctattggtgc gcatattgtc
     4681 agcaaggtac aaacgctgtt ggcaagccag agtaaccccg gacgcacgct gcgagcggca
     4741 cgtagtgtgg atgccttaca cgccgcattt aatcaataac ctttgaaaac aggacgtaac
     4801 aatgaaactt aacgacagta aattattccg ccagcaggcg ttgattaacg gggaatggct
     4861 ggacgccaac aatggcgagg tcatcgatgt caccaatccg gcgaacggcg acaagctggg
     4921 tagcgtaccc aaaatgggcg ctgatgaaac ccgcgccgct atcgacgccg ccaaccgcgc
     4981 tctgcccgcc tggcgtgcgc tcaccgccaa agaacgcgcc aacattctgc gcaactggtt
     5041 caatttgttg atggagcatc aggacgattt agcgcgcctg atgaccctag aacagggcaa
     5101 accgctggct gaagcgaaag gcgaaattag ctacgccgcc tcctttattg agtggtttgc
     5161 tgaagaaggc aaacgcattt atggcgacac cattcctggt catcaggccg ataagcgcct
     5221 gattgttatc aagcagccga ttggcgtcac cgcagctatc acgccgtgga acttcccggc
     5281 ggcgatgatt acccgcaaag ccggtccggc gctggcggca ggctgcacca tggtgctgaa
     5341 gcctgccagt cagacgccgt tctctgcgct ggcgctggcg gagctggcga ttcgcgcggg
     5401 cattccggct ggggtattta acgtggtcac cggttcggcg ggcgcggtgg gtaacgaact
     5461 gaccagcaac ccgctggtgc gcaagctgtc gtttaccggt tcgaccgaaa ttggccgcca
     5521 gttaatggaa cagtgcgcga aagacatcaa aaaagtgtcg ctggagctgg gcggcaacgc
     5581 gccgtttatc gtctttgacg atgccgacct cgacaaagcc gtggaaggtg cgctgtcctc
     5641 gaaattccgc aacgccgggc aaacctgcgt ctgcgccaac cgtttatacg tgcaggacgg
     5701 cgtgtatgac cgctttgccg aaaaattgca gcaggcggtg agcaaattgc acatcggcga
     5761 cgggctggag aaaggcgtca ccatcgggcc gctgatcgat gaaaaagcgg tagcaaaagt
     5821 ggaagagcat attgccgatg cgctggagaa aggtgcgcgt gtggtttgcg gcggtaaagc
     5881 ggatgaacgc ggcggcaact tcttccagcc gaccattctg gtggacgttc cagccaacgc
     5941 caaagtgtcg aaagaagaga cgttcggccc cctcgcccca ctgttccgtt ttaaagatga
     6001 agccgatgtg attgcgcaag ccaatgacac cgagtttggc cttgccgcct atttctacgc
     6061 ccgtgattta agccgcgtct tccgcgtggg cgaagcgctg gagtacggca tcgtcggcat
     6121 caataccggc atcatttcca atgaagtggc cccgttcggc ggcattaaag cctcgggtct
     6181 gggtcgtgaa ggttcgaagt atggcatcga agattactta gaaatcaaat atatgtgcat
     6241 cggtctttaa ctggagaacg cgaatgagca gcaataaaga gttaatgcag cgccgcagtc
     6301 aggcgattcc ccgtggtgtt gggcaaattc acccgatttt cgctgaccgc gcggaaaact
     6361 gccgggtgtg ggacgttgaa ggccgtgagt atcttgattt cgcgggcggg attgcggtgc
     6421 tcaataccgg gcacttgcat ccgaaagtgg tggctgcggt ggaagcgcag ttgaaaaaac
     6481 tgtcgcacac ctgcttccag gtgctggcct acgagccgta tctggagctg tgcgagatta
     6541 tgaatcagaa ggtgccgggc gatttcgcca agaaaacgct gctggttacg accggttccg
     6601 aagcggtgga aaacgcggta aaaatcgccc gcgccgccac caaacgtagc ggcaccatcg
     6661 cttttagcgg cgcgtatcac gggcgcacgc attacacgct ggcgctgacc ggcaaggtga
     6721 atccgtactc tgcgggcatg gggctgatgc cgggtcatgt ttatcgcgcg ctttatcctt
     6781 gcccgctgca cggcataagc gaggatgacg ctatcgccag catccaccgg atcttcaaaa
     6841 atgatgccgc gccggaagat atcgccgcca tcgtgattga gccggttcag ggcgaaggcg
     6901 gtttctacgc cgcgtcgcca gcctttatgc agcgtttacg cgcgctgtgt gacgagcacg
     6961 ggatcatgct gattgccgat gaagtgcaga gcggcgcggg gcgtaccggc acgctgtttg
     7021 cgatggagca gatgggcgtt gcgccggatc tcaccacctt tgcgaaatcg atcgcgggcg
     7081 gcttcccgct ggcgggcgtc accgggcgcg cggaagtgat ggatgccgtc gctccagacg
     7141 gtctgggcgg cacctatgcc ggtaacccga ttgcctgcgt ggcggcgctg gaagtgttga
     7201 aggtgtttga gcaggaaaat ctgctgcaaa aagccaacga tctggggcag aagctaaaag
     7261 acggattgtt ggcgatagcc gaaaaacacc cggagatcgg cgacgtacgc gggctgggag
     7321 cgatgatcgc catcgagctg tttgaagatg gcgatcacag caaaccggac gcaaaactca
     7381 ccgccgggat cgtggcgcgc gcccgcgata aaggcctgat tcttctctcc tgcggcccgt
     7441 attacaacgt gctgcgcatc cttgtaccgc tcaccattga agacgctcag atccgtcagg
     7501 gtctggagat catcagccag tgttttgctg aggcgaagca gtagctatgt ccgtttgtaa
     7561 gagttaaccg ttgctaaggg aatgataacg atgcagtaaa aaataataac gatgaggcga
     7621 acgacaaagt taaaaacgac tgcggacagt cccctcgccc ctctggggag agggttaggg
     7681 tgaggggtaa aacacgcggc taaaccctcc cctggccctc tcgctgagtg agaaggagaa
     7741 aaccatcccg ctttcgtttt taagattttg ttgaccgtct caattaaacg cgtgacccgg
     7801 cgcgggaaat gtcggggcgc tctcccaagt gacacacttt cgagaggatt caggatggga
     7861 caatcatcgc aaccacatga gttaggcggc gggctgaagt cacgccacgt caccatgttg
     7921 tctattgccg gtgttatcgg cgcaagtctg tttgtcggtt ccagcgtcgc catcgccgaa
     7981 gcgggcccgg cggtattact ggcctatctg ttcgccggat tactggtggt tatgattatg
     8041 cggatgttgg cggaaatggc ggttgccacg cccgataccg gttcgttttc cacctatgcc
     8101 gataaagcca ttggtcgctg ggcgggctat accatcggct ggttgtactg gtggttttgg
     8161 gtactggtta tcccactgga agccaacatc gccgctatga tcctgcactc gtgggttcca
     8221 ggcattccca tctggttatt ttccctcgtc attaccctcg ccttaactgg cagtaattta
     8281 ttaagcgtta aaaactacgg cgaatttgag ttctggctgg cactgtgcaa agtcatcgct
     8341 atcctggcct ttattttcct tggtgcagtc gcaattagcg gtttttaccc gtatgccgac
     8401 gtgagcggga tctcaagatt gtgggatagc ggcggcttta tgcccaacgg cttcggtgcg
     8461 gtattaagcg cgatgttgat caccatgttc tcgtttatgg gcgcagaaat tgtcaccatt
     8521 gccgccgcgg aatccgacac gccggaaaaa catattgtcc gcgctaccaa ctcggttatc
     8581 tggcgtattt ctatcttcta tttgtgctct atttttgtcg tagtggcgtt aataccgtgg
     8641 aatatgccgg ggctgaaagc cgttggttct tatcgctcgg ttctggaatt gctcaatatt
     8701 ccccatgcaa aattaatcat ggactgcgtg atattacttt ccgtaaccag ctgtctgaac
     8761 tcggcgctgt ataccgcgtc aaggatgctc tactccttaa gccgtcgcgg tgatgcgccc
     8821 gcggtaatgg gcaaaatcaa ccgcagtaaa accccgtatg tggcggtgtt actctccacc
     8881 ggagcggcat ttttaacggt ggtggtgaac tattacgcac ctgcgaaagt gtttaaattc
     8941 ctgatagaca gctccggtgc tatcgccctg ctggtttatt tagtcatcgc cgtttcacag
     9001 ttgcggatgc gtaaaattct gcgagcagaa ggaagcgaaa ttcgcttgcg catgtggctt
     9061 tacccgtggc tcacctggct ggtaataggc tttattacct ttgtgttggt agtgatgcta
     9121 ttccgcccgg cgcaacagtt agaagtgatc tctaccggct tattagcgat agggattatc
     9181 tgtaccgtgc cgattatggc gcgctggaaa aagctggtat tgtggcaaaa aacacccgtt
     9241 cataatacgc gctgataatg atcaggagtc acaccatgac cattacgtct ctggatggct
     9301 atcgctggct gaagaacgat attattcgcg gtaattttca accggatgaa aaattacgaa
     9361 tgagtttgct gacatcgcgt tatgcacttg gcgttgggcc gttacgggaa gctctttcgc
     9421 aactggtggc ggaacggctg gtcacggtgg tgaatcaaaa agggtatcgg gtggcgtcta
     9481 tgtcagagca ggagctgctc gatattttcg acgcccgcgc caatatggaa gcgatgctgg
     9541 tcagtctggc gattgcccgc ggtggcgatg aatgggaggc agacgttctc gcaaaagcgc
     9601 atctgctgag taagctggag gcctgtgacg ccagcgagaa aatgcttgat gagtgggatc
     9661 tgcgtcatca ggcgtttcat acagcaattg tggcgggctg tggttctcac tatttgctgc
     9721 aaatgcgtga acggttgttt gatctggcgg cgcgctatcg atttatctgg ctgcggcgaa
     9781 cggtgctttc ggtagaaatg ctggaggata aacacgatca gcaccagacc ctgactgcgg
     9841 cggtactggc gcgagatacc gcgcgcgcca gtgagttaat gcgccagcat ttactgacgc
     9901 caattcccat tatccagcag gcgatggctg gcaattaact actcttccgg aatacgcaac
     9961 acttgccccg gataaatttt atccgggctt tttagcatcg gtttattcgc ttcgaagatt
    10021 ttattgtaca gattagcgtt accgtagacc tgtttggaaa tggcactcag agtgtcgcca
    10081 gacttaacgg tataaaactg gctggcagtg gctggtgtcg ccgttttcac ctgatcatca
    10141 acactggcaa taccggaaat attccccacc gcaacaagga ttttctcctt cgcctcctga
    10201 ctcaggccgt caccagtgac cgtcgctttg ccgtcggcaa tttgaatgtt cactttatcg
    10261 gcatcaggta taccggtttt gctcagatgc tcctgcacct tcttcgcctg atcgtcttta
    10321 tcgtgctgac ctgtaaccgc gtcccagagt ttttctccgg catctttcac aaaattgaac
    10381 agacccatag ctacctcatt cgttaacgga aacactcaga aagtgtagca gaggaacgcg
    10441 gcgcgttaac gccgcggggg tgagcacagg tttaatcgcg ggtttgcacc cagaacgcgt
    10501 gaatcaaacc ggggatatac ccaagcagtg tcagcagaat attaataatg aacgcccaac
    10561 cgaacccttt accgagcagc acgccgagcg gcggcagaat gatggtgatg acgattctcc
    10621 agaaacccat atgtactc
//
LOCUS       DAJOSY010000132        10601 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJOSY010000132 DAJOSY010000000
VERSION     DAJOSY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.132"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            166..1533
                     /gene="dcuC"
                     /locus_tag="OHN27_003795"
     CDS             166..1533
                     /gene="dcuC"
                     /locus_tag="OHN27_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCQ2288161.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1577..2074)
                     /gene="sspB"
                     /locus_tag="OHN27_003796"
     CDS             complement(1577..2074)
                     /gene="sspB"
                     /locus_tag="OHN27_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCQ2288162.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2080..2718)
                     /gene="sspA"
                     /locus_tag="OHN27_003797"
     CDS             complement(2080..2718)
                     /gene="sspA"
                     /locus_tag="OHN27_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCQ2288163.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3113..3505)
                     /gene="rpsI"
                     /locus_tag="OHN27_003798"
     CDS             complement(3113..3505)
                     /gene="rpsI"
                     /locus_tag="OHN27_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCQ2288164.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3521..3949)
                     /gene="rplM"
                     /locus_tag="OHN27_003799"
     CDS             complement(3521..3949)
                     /gene="rplM"
                     /locus_tag="OHN27_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCQ2288165.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4168..5295)
                     /gene="zapE"
                     /locus_tag="OHN27_003800"
     CDS             complement(4168..5295)
                     /gene="zapE"
                     /locus_tag="OHN27_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCQ2288166.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5489..5887
                     /locus_tag="OHN27_003801"
     CDS             5489..5887
                     /locus_tag="OHN27_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCQ2288167.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6041..7408
                     /gene="degQ"
                     /locus_tag="OHN27_003802"
     CDS             6041..7408
                     /gene="degQ"
                     /locus_tag="OHN27_003802"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCQ2288168.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7498..8565
                     /gene="degS"
                     /locus_tag="OHN27_003803"
     CDS             7498..8565
                     /gene="degS"
                     /locus_tag="OHN27_003803"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCQ2288169.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8628..9566)
                     /gene="mdh"
                     /locus_tag="OHN27_003804"
     CDS             complement(8628..9566)
                     /gene="mdh"
                     /locus_tag="OHN27_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCQ2288170.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            10001..10471
                     /gene="argR"
                     /locus_tag="OHN27_003805"
     CDS             10001..10471
                     /gene="argR"
                     /locus_tag="OHN27_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCQ2288171.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2617 a         2695 c         2628 g         2661 t
ORIGIN      
        1 cttacaaaat aatcataagc gcatattttt taatgaaaaa tcacctcatc cacaattaaa
       61 aacacgacat ccgcaccata aatagccttg caaaaaatat aacatcgttg ttttcaatct
      121 gccgtttatg ggattgaccg ttttcttttg acacggagtt caacaatgtt cggcataatt
      181 atatctgtca tcgtattaat tacgatgggc tatttgatcc tgaaaaacta caaacctcag
      241 gtggtgctgg ctgccgcagg tatcttcctg atgatgtgcg gtgtctggtt agggttcggt
      301 ggtgtactcg atcccgccaa aagcagcggc tacttgatcg tcgatattta taatgaaatc
      361 ctgcgcatgc tgtccaaccg cattgccgga ttggggctgt cgattatggc ggtgggcggt
      421 tatgcccgct acatggagcg cataggggcc agtcgcgcga tggtgagctt gttaagccgc
      481 ccgttaaaac tcattcgctc gccgtatatt attctgtcgg caacttacgt catcggccaa
      541 atcatggcgc agtttattac cagcgcctcc ggtctgggta tgttgctgat ggtcacctta
      601 tttccgacgc tggtgagtct gggagtaagt cgcctctctg cggtggcggt tatcgcaacc
      661 acaatgtcca ttgagtgggg aattctggaa acgaactcca tttttgcagc ccaggtagcg
      721 ggaatgaaaa ttgccacata cttcttccac taccagcttc cggtcgcctc ttgcgtcatt
      781 atctcggtgg cgatctccca ctttttcgtg caacgcgctt ttgacaaaaa agataaaaat
      841 atcaatcacg aacaggcaga gcaaaaagct ctcgataatg tcccgccgct ctattacgcc
      901 attttacctg tgatgccgtt aatcttgatg ctcggctcgc tattccttgc ccacatcggg
      961 ctgatgcagt cagaactgca tctggtggtg gtgatgttac tgagtttgac ggtgacgatg
     1021 tttgttgagt tcttccgcaa gcataacttg cgcgaaacaa tggacgatgt gcaggcgttt
     1081 tttgacggca tgggtacgca gtttgccaac gtggtaacgc tggtggtcgc gggtgaaata
     1141 tttgcgaaag gcttaacgac gattggcact gtcgatgcgg ttatcagggg tgcggagcat
     1201 tctggtctgg gcggtattgg cgtgatgatt attatggcgc tggtcattgc catttgtgcc
     1261 attgtgatgg gctcaggcaa tgcgccgttt atgtcatttg ccagtcttat tccgaatatc
     1321 gcagccggac tacatgtacc agcggttgta atgattatgc caatgcattt tgccacgacg
     1381 ctagcgcgcg cggtttcacc gattactgcg gtggtggtcg ttacgtcagg aattgccggc
     1441 gtttcgcctt ttgcggtggt gaagcggaca gcgatcccca tggcagtcgg tttcgtggtg
     1501 aatatgattg ccacaatcac gctattttat taagtcatta aaaagacaaa acaggccacc
     1561 atggcctgtt tttgtattac ttcacaacgc gtaatgccgg tcgaccaccg cgtggtggct
     1621 gcggtggttc atcgtcagga tgagtgtcat catcgtgatc tggcttgtcg ccatcaataa
     1681 ccgacataac ggtttcgttg tctgccgatg cctcttcatc attcatgatg ctggtatctt
     1741 catcgtaggc agcttcaggc tcaaacatcg tgcctgcgcc attttcacgg gcgtagatag
     1801 ccagcacggc agccagcggc acagaaacct gacgcggaat gccaccaaag cgcgcgttaa
     1861 agcgcacctc atcattcgcc agttccagat tgccgacagc acgcggcgca atgttgagta
     1921 cgatttgccc gtcacgcgca tattccatag gaacctgcac gccagggagc gtcacatcca
     1981 ccaccaggtg cggcgtgagc tggttatcca gcaaccactc atagaatgca cgcagcagat
     2041 agggacgacg tggtgttagc tgtgacaaat ccatacagat taactccggc ccagacgcat
     2101 ttcacgttct gcttcagtta aagaagcaag gaaagagtca cgctcaaaga cgcgggtcat
     2161 atagcctttc agctctttcg cacccgggcc gctgaactcg atacccagtt gcggcagacg
     2221 ccacagcagc ggagcaaggt agcaatcgac caggctgaac tcatcgctca ggaagtacgg
     2281 cttctgaccg aagaccggcg caatcgccag cagttcttcg cgcagttgct tacgtgcggc
     2341 atctgcttca gaagctgaac cgttgatgat ggtgttcatc agcgtgtacc agtctttttc
     2401 gatgcgatgc atgtacagac ggctttcacc gcgagctacc gggtaaacag gcatcagtgg
     2461 cggatgcggg aaacgctcat ccagatattc cataatgatg cgagattccc acagggtcag
     2521 ctcacgatcc accagggtcg gaacgctctg attcgggttg aggtcaatca gatcctgagg
     2581 cggattgtcc ttttccacgt gttcgatctc gaaacttaca cctttctcag ccagcacaat
     2641 gcggacctga tggctataga tgtcagtagg accggaaaac agcgtcatta ccgaacgttt
     2701 gttggcagcg acagccatga aaacctccag gtatagtcag aatttttact gctaccagcc
     2761 accaggtggc cagtcagaag ttgtgttacc cagtaaggaa cgactctctt tgttcgaaaa
     2821 tcaaacaaaa aatgagcaat acccgacatt tgggcagaaa attggatgat agtttaccag
     2881 attttgcgac ctttgtggtg agtcgatgcc ggaaatgggg aaaaagagat gcgctttagt
     2941 ctgaaatagt tgacttagtc ccttattggc gatgtggttt ttattttgcc tgtctgtcag
     3001 acggcagcaa aaagtaactt tccatttttt acgctgattc agaatttaac cataaaaaaa
     3061 cccgccgaag cgggtttttt cgcaaattgt tttctgccgg agcagaagcc aattaacgtt
     3121 tggagaactg cggacgacga cgtgctttac gcagaccgac tttcttacgt tcaacctgac
     3181 gagcgtcacg agtaacgaag ccagctttac gcagttcaga acgcagggac tcgtcgtatt
     3241 ccatcagagc gcgggtgata ccgtgacgga tcgcaccagc ctgaccagag ataccaccac
     3301 ctttaacggt gatgtacagg tccagtttct caaccatgtc gaccagttcc agcggctgac
     3361 gaactaccat gcgagcagtt tcacgaccga agtactgttc cagagaacgt tggttgatta
     3421 cgattttacc gttgcccggt ttgatgaaaa cgcgagctgc ggaacttttg cggcgaccag
     3481 tgccgtagta ttgattttca gccattgcct ataatcccga ttagatgtca agaacttgcg
     3541 gttgctgtgc cgcgtggttg tgctcgttac ccgcgtaaac tttcagttta cggaacatag
     3601 cacgacccag cgggcctttt ggcaacatgc ctttaaccgc gatttcaatc acacgctcag
     3661 gacggcgagc aatcatctct tcaaaggtcg cttgtttgat accaccgatg tggccggtgt
     3721 ggtgatagta cactttgtca gtacgcttgt tgccggttac agcaactttg tcagcgttca
     3781 gaacgatgat gtaatcaccg gtatctacgt gcggagtgta ttccgctttg tgcttaccgc
     3841 gcaggcgacg agccagttca gtagccagac ggcccagagt tttaccggtc gcgtcaacaa
     3901 cataccagtc gcgttttacg gtttctggtt tagctgtaaa agttttcatt aaaagcttac
     3961 ccaataaata gttacacgtt ggtgaacacc caaacgtctt caattgttga ggttcacacg
     4021 acaaagtccg gcaaacctac cccttcgaat agcctatgcc agcacacaaa aagttttggg
     4081 aaaaaaactt tcttgtaacg tggggtcgca ggattataga gaagtcgggg tcaaagatcg
     4141 accccttttt gtgatttgtg acaggtttta acccgccaaa tgctcgcgct tcagatactc
     4201 ttcgctttgc atctcttgca gacgtgacag gcaacgctgg aactcaaact tcagccgatc
     4261 gccctgataa atttcataca gcggcacttc tgcactcacc actaatttga catggcgctc
     4321 gtaaaactca tccaccagcg caataaagcg ccgcgcttcg ctctccatca accgcgtcat
     4381 aactggtaca tcaaacaaca tgaccgtatg aaagagacgt gagagcgcaa tatagtcatg
     4441 ctgactgcgg gcgtcgacgc acagcgtagt aaaagagacc gccagcgtct ggttctcgac
     4501 gcccattgtt gctaatggcc gatggttgat ttctaacgtc ggtgaatttt ctcgtttccc
     4561 ccccgccagc gccaaccata gtttatccat ttgcgcccgg gtttcatcgt gaagtggcga
     4621 aagccacaga tgcgcctgag tgagtgtacg cagacgataa tcaacaccag cgtccacgtt
     4681 cattacatca caatgctgtt taatggcatc gattgcaggc agaaaacgcg cacgttgcag
     4741 gccatttcga taaagttcat ccggcggaat atttgacgtc gctaccaggg taataccgcg
     4801 agcgaacagg gctttcatca gaccgccaag tagcatggca tcggtaatat cagaaacaaa
     4861 aaattcgtca aaacagagca cgtcagtttc ggctttaaag cgatcggcaa taatttccag
     4921 cggatcggtc tgcccctgta aggcagttag ctcttcatgc acccgcagca taaaacggtg
     4981 aaagtgcagg cgctgtttcc gctctcccgg caggctttga tagaaaaggt ccatcagcca
     5041 ggttttcccg cgtcctacac cgccccacat atataagcca cgcactggcg tatgctttgt
     5101 gtcttcgcgt ttaccccaca gcttaccgac ccgcgccatt agcccactcg tcctgggggc
     5161 tggtggcctg ctattgatga gttcttgata aataatttcc aggcggctga cggcctcttt
     5221 ttgaacgtcg tcgggttgat ggctgccttc attgagcgcc ttcaggtatt gcgatgttgg
     5281 ggtaacgctt tgcatgatct tattgttatt ccttgaataa tcggtgcgcc gttgttcacg
     5341 gttgacgaaa aaaaggccgt tctacactac gcgatatgca gtcgggattc cacttctgtg
     5401 gaattaacgg ttatagtggc ataatcagcc gcaggcatgg agcctgaagc caacacccta
     5461 cggaaacaaa agacaacggg agatgttcat gacctgggaa tatgcgctaa ttgggttagt
     5521 cgtcggcatc attattggtg ctgtggccat gcgttttggt aatcgtaaac tacgccagca
     5581 acaggcgttg cagtacgaac tggaaaagaa taaagctgaa ctggacgagt atcgcgaaga
     5641 gctggttagc cactttgccc gcagcgcgga attactggat accatggcgc acgactatcg
     5701 ccagctgtat cagcacatgg caaaaagctc cagcagcctg ctgccggaac tgtctgctga
     5761 agcaaacccg ttccgtaatc gtctggcaga gtctgaagcc agcaacgatc aggcaccggt
     5821 gcagatgcct cgcgactatt ctgaaggcgc atccggcctg ctgcgtactg gcgcgaagcg
     5881 cgactaattt atttttcggg cgcagccatt gcgccctcct cttctctccc tccccgacta
     5941 tcatttaatc tggtgtctca ttgttagccg tctgaaaatt caataacatc aaactgtttt
     6001 gaatctcttt tcttatcatt caggtacgag agcaggaata atgaaaaaac aaacccagct
     6061 gttgagtgca ttagcgttaa gtgtcgggtt aactctctcg gcgtcatttc aggccgtcgc
     6121 gtcgattcca ggccaggttg ccgatcaggc ccctctcccc agtctggctc caatgctgga
     6181 aaaagtgctt ccggcagtgg tgagcgtacg ggtggaagga acggccagtc agggacagaa
     6241 aatcccggaa gaattcaaaa agttttttgg tgatgattta ccggatcaac ctgcacaacc
     6301 cttcgaaggt ttaggctccg gtgtcatcat caacgccagt aaaggctatg tgctgaccaa
     6361 caaccatgtg attaatcagg cacagaaaat cagtattcag ctcaatgatg ggcgcgagtt
     6421 tgatgcaaaa ctgattggta gcgatgacca gagcgatatc gccctgttac aaattcaaaa
     6481 cccgagcaaa ttaacgcaaa tcgctattgc cgactccgat aaattgcgcg tcggtgattt
     6541 tgccgtagcg gtcggtaacc catttggcct tgggcaaacc gccacctctg gcattgtttc
     6601 cgcattaggc cgcagcgggt tgaatcttga aggtctggaa aactttatcc agacagatgc
     6661 ttccattaac cgcggtaact ccggcggtgc actattaaac cttaacggtg agttaattgg
     6721 catcaacact gcaatccttg cgcctggcgg cgggagcgtc gggattggat ttgccatccc
     6781 cagtaatatg gcgcgaacac tggcgcagca gcttatcgac tttggtgaaa tcaaacgcgg
     6841 tttgttaggc atcaaaggca ccgagatgag tgccgatatc gccaaagcct tcaaccttga
     6901 cgtgcagcgt ggcgcgtttg tcagcgaagt gttgccaggt tctggctcgg caaaagcggg
     6961 cgtcaaagcg ggcgatatta ttaccagcct caacggcaaa ccgctgaata gctttgctga
     7021 gttgcgctct cgtatcgcga ccaccgagcc gggcacgaaa gtgaagcttg gcctgctgcg
     7081 taacggcaaa ccactggaag tagaagtgac gctcgatacc agcacctctt cgtcggccag
     7141 cgctgaaatg atcacgccag cgctggaagg tgcaacgttg agcgatggtc agctaaaaga
     7201 tggcggcaaa ggtattaaaa tcgatgaagt tgtcaaagga agcccagctg ctcaggctgg
     7261 cttgcaaaaa gacgatgtga tcattggcgt caaccgcgat cgggtgaact cgattgctga
     7321 aatgcgtaaa gtgctggcgg caaaaccggc catcatcgcc ctgcaaattg tacgcggcaa
     7381 tgaaagcatc tatctgctga tgcgttaatg tcgtaaaccg ggcatcaggc ttacgtgtga
     7441 tgtccggtta actcgtggta tgctgctgcc gttccctttt ttaatgacgc ctccatcatg
     7501 tttgtgaagc tcttacgttc cgttgcgatt ggattaattg tcggcgctat tctgctggtt
     7561 gccatgcctt cgctgcgcag ccttaacccg ctttccactc cgcaatttga cagtaccgat
     7621 gagacgcctg ccagctataa tctggcggtt cgccgcgccg cgccagcggt ggttaacgtt
     7681 tacaaccgtg gtttgaacac caactctcac aaccagcttg agatccgcac cctgggatcc
     7741 ggtgtaatta tggatcaacg cggttatatc atcaccaata aacacgtcat caacgacgcc
     7801 gatcagatca tcgtcgcctt acaggatgga cgtgtatttg aagcattgct ggtgggatct
     7861 gactctctaa ccgatctggc ggtacttaaa attaatgcca ctggcggttt acctaccatt
     7921 ccaattaatg cacgtcgcgt accgcacatt ggcgacgtag tactggcgat cggtaacccg
     7981 tacaacctcg ggcagaccat tacccagggg attattagtg ccacgggtcg aatcggtctg
     8041 aacccgaccg ggcggcaaaa cttcctccaa accgatgctt ccattaacca cggtaactct
     8101 ggcggcgcgc tggtgaactc gctgggcgaa ctgatgggca ttaatacgct gtcgtttgat
     8161 aagagtaacg atggcgaaac gccggaaggt atcggctttg cgattccttt ccagttagca
     8221 accaaaatta tggataagct gatccgcgat ggtcgcgtga tccgcggcta cattggtatc
     8281 ggcggacgtg agatcgcacc actgcacgcg cagggcggtg gtatagatca actgcaaggg
     8341 atcgtggtta atgaagtgtc acctgacggc ccggcggcga atgcgggtat tcaggtcaac
     8401 gatctgatta tttcggtgga taacaaaccg gccatctctg ctctggagac gatggatcag
     8461 gtggcggaaa ttcgccctgg ttcggtgatc cctgtagtag tgatgcgtga tgataagcag
     8521 ttaacgctgc aggtcaccat tcaggaatat ccggcaacca attaagtcgt gcgctcaaaa
     8581 caaaaaaccg gagtctgtgc tccggttttt tattatccgc taatcaatta cttattaacg
     8641 aactcttcgc ccagggcgat atctttcttc agcgtatcca gcataccttc cagcgcgttc
     8701 tgttcaaatg cgctcagggt accgatagat ttacgctctt ccacgccgtt tttacccagc
     8761 agcagcggtt gagagaagaa acgggcgtac tgaccgtcgc cttcaacgta ggcacattcg
     8821 acaacgcctt gttcgccctg cagtgcacga accagagaca gaccaaaacg tgcagctgcc
     8881 tggcccatag acagggttgc agacccgcca ccggccttcg cttcaaccac ttcagtaccc
     8941 gcgttctgga tgcgtttggt cagatcagcc acttcctgct cggtaaaact aacgccagga
     9001 acctgtgaca gcagcggcag aatggtaaca ccagagtgac cgccaataac cggcacttca
     9061 acttcgcctg gctgtttgcc tttcagttcc gcaacaaagg tgttggaacg aatgatatcc
     9121 agcgtggtaa cgccgaacag tttgtttttg tcataaacac cggctttttt cagcacttca
     9181 gcagcaattg caactgtggt gttaaccggg ttagtgataa taccaatgca cgctttcggg
     9241 caggttttcg caacttgctg taccaggttt ttcacgatgc cggcgttaac gttaaacagg
     9301 tcggaacgat ccatacccgg tttacgcgct acgcctgcag agataagaac gacatctgcg
     9361 ccttccagcg ccggagtcgc atcttcacca gaaaaacctt tgattttcac agcagtaggg
     9421 atatggctca gatcgacagc cacaccggga gtcactggag cgatatcata cagagagagt
     9481 tctgaacctg aaggcagttg ggtttttaac agtagtgcaa gcgcctggcc aataccgcca
     9541 gcagcgccga ggactgcgac tttcatccta aactccttat tatattgata aactaagata
     9601 tgttgctccg ctgccgcgac cttaatccac aaaattgccg tttacaatga ccacatctca
     9661 agaatgtgta gtcacgcaag tttagcgttt atgcatttaa ttgccgtaat caggaaccta
     9721 acttacgtaa ttaacagtcg cttatgcatt agcgcaacat tccaacaggt ggtgacaata
     9781 taccctaccg ttcagccaaa acaacatcaa tttgataaca attaatttac ttttaagcag
     9841 aatttgcatg ccgtgacgca ggcatgtttc tcaataacga aatttgataa aatcccgctc
     9901 tttcataaca ttatttcagc cttcttcagg gctgactgtt tgcataaaaa ttcatctgta
     9961 tgcacaataa tgttgtatca accaccatat cgggtgactt atgcgaagct cggctaagca
    10021 agaagaacta gttaaagcat ttaaagcatt acttaaagaa gagaaattta gctcccaggg
    10081 cgaaatcgtc gccgcgttgc aggagcaagg ctttgacaat attaatcagt ctaaagtctc
    10141 gcggatgttg accaagtttg gtgctgtacg tacacgcaat gccaaaatgg aaatggttta
    10201 ctgcctgcca gctgaactgg gtgtaccaac cacctccagt ccattgaaga atctggtgct
    10261 ggatatcgac tacaacgatg cagttgtcgt gattcatacc agccctggcg cggcgcagtt
    10321 aattgctcgc ctgctggact cactgggcaa agcagaaggt attctgggca ccatcgctgg
    10381 cgatgacacc atctttacca cccctgctaa cggtttcaca gtcaaagacc tgtacgaagc
    10441 gattttagag ctgttcgacc aggagcttta atctctgccc cgtcgtttct gacggcgggg
    10501 aaaatgttgc ttatccctct caaccccctg ctttcccctg cgattaattt aacgaatagt
    10561 gcgttttact gcgacatgtc attcacacaa tgaatacata a
//
LOCUS       DAJOSY010000133        10496 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJOSY010000133 DAJOSY010000000
VERSION     DAJOSY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.133"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1351
                     /gene="paoC"
                     /locus_tag="OHN27_003806"
     CDS             <1..1351
                     /gene="paoC"
                     /locus_tag="OHN27_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HCQ2288172.1"
                     /translation="ATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLY
                     AGANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDT
                     QVDPADPTRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNL
                     LEKSGARVHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFP
                     VSAGSGGQWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAMLHE
                     ATAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRIL
                     NPKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFL
                     DDTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRIRDYPITLDKLLDKLPDVV"
     gene            1361..2317
                     /gene="paoD"
                     /locus_tag="OHN27_003807"
     CDS             1361..2317
                     /gene="paoD"
                     /locus_tag="OHN27_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HCQ2288173.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTK
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETTQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            2368..2706
                     /locus_tag="OHN27_003808"
                     /pseudo
     CDS             2368..2706
                     /locus_tag="OHN27_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3524..4738
                     /locus_tag="OHN27_003809"
     CDS             3524..4738
                     /locus_tag="OHN27_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HCQ2288174.1"
                     /translation="MYMSVFHNWLLDIASGNYFIYIKRLSANDTGATGGHQVGLYIPS
                     NIVEKLFPSINHTRELNPSVFLTAHVSSHDCPDTQARAIYYNNRYFGKTRNEKRITRW
                     GRGSPLQNPENTGALTILAFRLNEQNTDCSEVDIWVCVNPDEEDIIESAIGEIIPGTL
                     ISGPAGQILGGLSLQQTPVNHKYVIPEDWKKRFPSGNEIIQYAAGHYAKNSKDPDEQL
                     IDRRRVEYDIFLLVEELHVLDIIKKGFDSVDEFIALANSVSNRRKSRAGKSLELHLEK
                     LFIEHGLRHFSTQAVTEGNKKPDFLFPSAEAYHNVEFPVENLRMLAVKTTCKDRWRQI
                     LNEADKIHQVHLFTLQEGVSSAQYREMKDAGVRLVVPSTLHKKYPEAVREELITLGAF
                     ITELIELYAELS"
     gene            complement(4773..6176)
                     /locus_tag="OHN27_003810"
     CDS             complement(4773..6176)
                     /locus_tag="OHN27_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCQ2288175.1"
                     /translation="MLKEAEAEEKLRQENDKKLIEKVLEIYDQKYVAELLRKVGKNEW
                     SRETINRWINGKCLPKSLTSVEESLLRKMLPEPPANHPEYAFRFIDLFAGIGGIRKGF
                     EAIGGQCVFTSEWNKDAVRTYKANWFNDEQVHKFNLDIREVTLSDKTDVLETDAYAYI
                     DEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKKPA
                     IFVLENVKNLKSHDKGKTFKVIMETLDELGYEVADAAEMGKNDPKIIDGKHFLPQHRE
                     RIVLVGFRRDLNIHKGFTLRDISRFYPEHRPSFGELLEPVVDSKYILTPKLWEYLYNY
                     AKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMATGEADFMNES
                     NQARRPRRLTPRECARLMGFEKPGGKPFRIPVSDTQSYRQFGNSVVVPVFEAVARLLE
                     PYILKAVSADAGKTGQP"
     gene            6613..7386
                     /locus_tag="OHN27_003811"
     CDS             6613..7386
                     /locus_tag="OHN27_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288176.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAATDDDELLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASQAEIILATVRGIVRGVFIADKWLKSTRENFPSLKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(7456..7584)
                     /locus_tag="OHN27_003812"
     CDS             complement(7456..7584)
                     /locus_tag="OHN27_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCQ2288177.1"
                     /translation="MEIATEEEKALLAAWKKYRVLLNRVDTSTAPDIEWPEEPDTV"
     gene            complement(7639..9498)
                     /locus_tag="OHN27_003813"
     CDS             complement(7639..9498)
                     /locus_tag="OHN27_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HCQ2288178.1"
                     /translation="MSGDLDILKETPSIRLKSAKGTAHLWFMNNDGSERGVVWSPENN
                     ESLGEIHIRAKNTKGESSGDFIVRHDGRVEARNLKITYKISAATAEFANTSTSSDNTT
                     VSIKGSQHTPLVLTSNNTIKNLSIGFKVDDVDQKYLGIAGDGDLYFGSYSDHTKNSKV
                     ITQAKLDSGVTVGGKTTFSDLATFNAGMAGSIEPETIDNKTIDLNDLIIANTVAGSVK
                     YYQCKTVAGGAYITNKPDGVSGNFLLRVESTRKTTGSDYAIMQTLIGSDTKRIYVRFV
                     VNGSWTEWSQVVVSGWNQDVTVRSLTSTTPSKLGGGRVDVLGSTSDYSSMNCAVRGVD
                     STGTNSAWSVGTSKNTGKMLCLKNHRSSAQVLLNGDDGAVQLLSGTVNGATAQALTIN
                     KDEVNSTADLVIRKQTGTGNRFALLNSGNSELPVGIRVWGSSTRQNVFEVGTSAAYLF
                     YAQKTSAGQLFDVNGAINCTTLNQSSDRDLKDDILVISDATKAIRKMNGYTYTLRENG
                     MPYAGVIAQEVMEAIPEAVGSFTHYGEELQGPTVDGNELREETRYLNVDYAAVTGLLV
                     QFARETDDRVTALEEENTTLRENLATADTRISTLENQVSELVALVRQLTGSEH"
BASE COUNT         2665 a         2625 c         2455 g         2751 t
ORIGIN      
        1 cgccaccctt cagcacttgc gtatcggtgc tgaccagagc gggaaaatca ccgctatctc
       61 acatgaaagc tggtccggaa acctgcccgg cggcacgccg gaaacggcgg tacagcaaag
      121 cgaattactc tacgcagggg caaaccgtca taccggcctg cggctcgcca cgcttgattt
      181 gccggaaggg aacgccatgc gtgcgcccgg cgaagccccc ggtctgatgg cgctcgaaat
      241 cgcgatcgat gaactggcgg aaaaagcggg catcgatccc gtcgagtttc gcatcctgaa
      301 tgacactcag gttgaccccg ccgatccgac gcgccgcttc tctcgccgtc agcttattga
      361 gtgcttgcgc accggagcgg ataaatttgg ctggaagcag cgcaacgcca cacccggaca
      421 ggtgcgcgac ggggagtggc tagtcggcca cggcgtcgcg gcgggctttc gcaataatct
      481 gctggaaaaa tcgggtgctc gggttcacct cgaaccaaac ggcaccgtta ccgtggaaac
      541 ggacatgacc gacattggca ccggcagcta caccattctg gcccagacgg cagcggaaat
      601 gcttggcgta ccgctggagc aggttgcggt tcacctcggc gattccagtt tcccggtttc
      661 tgcgggttct ggtggacaat ggggcgcgaa tacctccacc tccggcgttt acgccgcctg
      721 tgtgaagctt cgcgaaatga ttgcctcggc agtcgggttt gatcctgagc agtcgcagtt
      781 tgccgacggc aagattacca acggtacccg aagcgccatg ctacatgagg ccaccgcagg
      841 cggcagactg acagcggaag agagcattga attcggaaca ctgagcaagg agtaccagca
      901 gtcgaccttt gccgggcatt ttgtggaggt cggcgtgcat agcgcgacgg gagaagttcg
      961 ggtccggcgt atgctcgctg tgtgtgctgc aggacgcatc ctgaatccga aaactgcacg
     1021 cagccaggtc attggcgcaa tgactatggg catgggcgcg gcactgatgg aggagctggc
     1081 ggtagatgac cgtttgggct acttcgttaa tcacgatatg gcggggtatg aggtgccggt
     1141 ccatgcggat atcccaaaac aggaggtgat tttcctggat gataccgacc ccatatcctc
     1201 cccgatgaag gccaaaggtg tcggtgagct gggcctgtgc ggcgtgagcg cggctatcgc
     1261 caacgcggtg tataacgcca ccggtattcg gatacgggat tatcccatca ctctggataa
     1321 gctgctcgat aagctgccgg atgtggttta aggaggaaca atgtcatacc cgctttttga
     1381 caaagacgaa cactggcata agccagagca ggcgtttctc accgatgacc accggaccat
     1441 tctgcgcttc gccgtagagg cgctaatgtc cggtaaagga gcggtgctgg tgacgctggt
     1501 ggagatacgc ggcggcgcgg cgcgcccgct cggggcgcag atggtggtgc gcgaagatgg
     1561 tcgttactgc ggttttgtct ctggcggctg cgtggaggcc gctgccgctt ttgaagcgct
     1621 ggagatgatg ggctcaggcc gcgatcgcga aattcgctat ggcgaaggtt cgccgtggtt
     1681 tgacatcgtt ctgccctgcg gcggtgggat cacgctgacg ctccataaac tacgctcggc
     1741 acagcctctg ctcgccgtgc tgaaccgtct ggaacagaga aaaccggcgg ggctgcgcta
     1801 cgatccgcaa gcacaatcgt tggtgtgcct gcccacgaaa acccgaacgg gctggaatct
     1861 caatggcttt gaggtggggt tcaggccatg cgtcaggctg atgatttacg gacgttctct
     1921 tgaggcgcag gcaaccgcga gtcttgcagc agccacaggc tatgacagcc atatcttcga
     1981 tctttttccg gcctcagcca gcgctcagat cgataccgat acggcggtca ttttgctgtg
     2041 ccatgatctc aaccgggagc tgccagtgtt gcaggccgcg cgagaagcaa aaccctttta
     2101 tctcggcgca ttgggcagct atcgaaccca cacgttacgt ctgcaaaagc tccacgagct
     2161 gggatggtcc agggaggaaa caacgcaaat ccgggcaccc gtcgggatat tccccaaagc
     2221 ccgggatgcg catactctgg cactctccgt gctggcagaa gtcgcctctg tacgtctcca
     2281 tcaggaggag gattcatgcc tgcccccgtc gtcctgatcc ttgcggccgg gcgtggagag
     2341 cgctttctcg cctccggggg aaatacccat aagtgtatcg gctggcgtca gtccccggag
     2401 gttgcgcctt atcgctggcc atttgaagaa aacgggagaa ctttcgacct tgcgattgaa
     2461 ccgcagatta cgactaatga tctgcgtctg atgttgaggc tggctcttgc cggcggagga
     2521 ataacaattg ccactcagga aactttcagg ccatatattg aaagcggtaa gcttgtatcg
     2581 ctgcttgatg actttcttcc acaatttccg ggcttctatc tgtatttccc acagcgtcgc
     2641 aatattgcac caaagctccg cgccctgatt gactacgtca aagaatggcg gcagcaattg
     2701 gcttaaatgt ctgcacctgc actgcctgat gtcagaacag tattttgatg aattgccaag
     2761 gttacaatgg cacaaatacg gcacaggagg aaacgtggtg tatttaaata tggggtaact
     2821 ctttgatttt aatggtgcga taataggagt cgaacctacg accttcgcat tacgaattat
     2881 aagaatccgc ttctaattca aagtattacc ccatcaacac tgcgctcaca cgtcccacca
     2941 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtcc
     3001 caccttgtat tatgacttgc atagccaatg aagataaagg tgacgacaaa cagcgcagca
     3061 gtcttctttt ctttcacact ttccccaatc agcatacata gcagtcctag agacagtggc
     3121 gtaaagtcat ggatgatcgg tggaaggagg cgctaatcct ttcatacaaa aaatatgtaa
     3181 aatcaataac ggctgtaaat cattcaatac tcgccctatc ggaagttcac cagccaaccg
     3241 cagcacgttc ttgcatacga cgtggctgcg gtttcaactc ccgccagctc caccaatcat
     3301 gattggacgg tgtaaggaca acaccaacaa aaacaggaag ttagcagtct cagcaggaca
     3361 ccgaccagac ggtgaggaga caaaaaagga tacgcaaagg agccgcggct ctcgagtgac
     3421 acaaaagccc gcttatgcgg gctttttgtt tttcccttaa gttcctagcc gcttatctat
     3481 aactatggaa aaatgttaac cctgactgta tgttaacaaa gggatgtata tgtcggtttt
     3541 tcataactgg ctgcttgata tcgcaagcgg gaattacttt atctacatca aacgcctttc
     3601 tgcaaacgac acaggcgcaa caggtggcca tcaggtcgga ctttatatcc cctcaaatat
     3661 cgttgaaaag ctttttccat ctatcaatca tactcgcgaa ctgaaccctt cagtcttcct
     3721 tactgcgcat gtatcatccc atgattgccc tgatacccaa gcacgcgcaa tttattacaa
     3781 caaccgttat tttggtaaga cccgaaacga aaaaagaatt acgcgctggg ggagaggaag
     3841 tccattacag aaccctgaaa atacaggagc cctcacaatt cttgctttca ggttaaacga
     3901 acagaacact gactgttccg aggtagatat atgggtctgc gtcaatcccg atgaagagga
     3961 tatcatcgag tctgctattg gcgaaatcat acctggaacc ctaatttccg gccctgccgg
     4021 acaaattttg ggcggattgt ctcttcagca aactccagta aatcataaat atgttattcc
     4081 tgaagactgg aagaagcgtt ttccttctgg aaacgaaatt attcaatatg ctgctggcca
     4141 ttatgctaaa aactccaagg atccagatga gcaactgatt gaccgtcggc gtgtcgagta
     4201 tgatattttt ctactcgtcg aggaattaca tgttcttgat attattaaga aaggattcga
     4261 ttctgtagat gagtttattg cattagccaa ctctgtcagt aatcgacgta aatcaagggc
     4321 aggcaaatca cttgaacttc acctagagaa gctttttatc gagcacggac tacggcattt
     4381 ctccactcag gcagttactg aaggtaataa aaaaccagat ttcctgtttc cttcagcaga
     4441 ggcatatcat aacgttgaat ttcctgtaga aaacttacgt atgctggcag taaagaccac
     4501 ctgcaaagat cgctggcgtc agatactgaa tgaagcagat aaaatccatc aggtacattt
     4561 atttacactg caagaaggtg tttcttcagc acaataccga gaaatgaaag atgcgggtgt
     4621 cagactcgtt gtaccatcaa ctttacataa aaaataccca gaagcagtta gagaagaatt
     4681 aataacgctc ggagcattca ttactgagct gatagagctt tacgctgaac tatcataggc
     4741 tgactcccgg cttaaaaggc cgggagatgt tctcaaggct gcccagtctt accagcatca
     4801 gcagaaacag ctttgaggat atagggttcc agaagtctgg caacagcttc aaatactggc
     4861 accacaactg agttaccgaa ctgccgatat gactgagtat ctgaaaccgg aatacggaat
     4921 ggtttccctc caggtttttc aaatcccata aggcgtgcgc actctcgggg agtcagcctg
     4981 cgtggtcgac gagcctgatt actctcattc ataaagtcag cctctcccgt tgccatatcc
     5041 caaccacgat caataagaat ttctgatccg tctttgtgat atctggcaga aagtgtgcgt
     5101 gcaatgcttt ccttattctc aggattgacc aacccaaagc caaaaccatt ccccttggct
     5161 gcatgctttt tggcgtagtt ataaaggtac tcccatagtt ttggcgtaag tatatattta
     5221 ctgtcgacta caggctccag caattcacca aatgatgggc ggtgttccgg ataaaaacga
     5281 ctgatatcac gcaaggtaaa ccccttgtga atattcagat ctcgtctaaa tccgaccaga
     5341 acaatgcgtt cacgatgctg aggcaaaaaa tgcttcccat cgataatctt tggatcgttt
     5401 tttcccattt ctgcagcatc ggcaacttcg tagcccagct cgtcaagggt ctccataatg
     5461 actttgaaag ttttaccctt atcatggctc ttcagatttt tgacattttc cagcacaaaa
     5521 attgccggtt tttttgctcg tataatacgt gccacgtcaa aaaaaagtgt tccttgtgct
     5581 tcacattcaa aaccatgcgc acgaccaagt gagtttttct tactaacacc agcaaggcta
     5641 aatggctggc acgggaaccc cgcaagaagc acatcatgat ccggcacatg ctcatcaata
     5701 tatgcataag catccgtttc caatacatcg gttttatcac tcagcgtgac ttcccgaata
     5761 tcgagattga atttatgcac ctgttcatcg ttaaaccagt tggccttgta tgtacgcaca
     5821 gcatctttat tccattcact ggtaaaaaca cactggcctc caatggcctc aaaacctttc
     5881 cgtatccctc caattccagc aaataagtca atgaagcgga aagcatattc cggatggttt
     5941 gcaggtggtt ctggtagcat cttacgcaga agagactctt ctactgaagt caacgatttt
     6001 ggtaaacact tgccattaat ccagcggtta atggtttcac ggctccactc attttttccg
     6061 acttttctca gtaattcagc cacatacttc tgatcataga tttccagcac tttctcgata
     6121 agctttttat cattttcctg tcgcagcttt tcttccgcct cggcttcctt cagcagatgc
     6181 tgtgccaaca cttcaaattc agacataatt cctccaaggg gtctaatggg tgaaacttta
     6241 tcactcattc aacccagaag gaaatgtttt atctggatat ttaaacagtg actacaacgt
     6301 aatctagcac tggtgatgct ttgttaggca tagagaatca ttctatatac gactaatgac
     6361 agaaaaacag cagacaagta gtttgttcat aaattaacgc atactatgtg tctacggttt
     6421 tcgagaccgg tccaatcatc aaacgaaaca taaaattagc tcacattatg aggaaaagta
     6481 tcttttttgt actatgtaaa ttcaaaggct tagcctcatt tctccgatgg ttttctcaac
     6541 actactggtt gtgagccctt gcaatgatca ttaatatacg tctcacaaat aattcttcat
     6601 agatattgca aaatggatat tactgagttt ccttctggag taattgaaca ccttggctgg
     6661 tatgtatacc gattgattga tccgagggac ggaagcacct tctatgtagg gaaaggcaaa
     6721 ggtaaccgcg tatttgccca tatgcgcggt gaagtggcag cgactgatga tgacgagtta
     6781 ctgagcaaca agctaaagca aattagagaa ataaggttag caggacttga agttatccat
     6841 gtcatccatc gacacggaat gactgatgaa aagacggcgt acgaagttga agcagcactt
     6901 attgatgcct accctgggtt aacgaatatc atgaatggtg ctggcagcaa tgaattcggc
     6961 gccgcgcatg tcaaagagtt gatagcaaca tatcagcccg aaaccataac atttcatcat
     7021 aaagcattaa tgatatccgt taacagaagt gcaaaggatt cagagcttta tgatgcggtt
     7081 cgatttagct ggcgcattaa tgtctctcgc gccagccaag cagaaatcat tcttgctact
     7141 gtaaggggga tcgttcgagg ggttttcatt gctgataaat ggctcaaatc aacacgtgaa
     7201 aatttccctt cgttgaaata ctgggacgag gatcctgact ttgaggcaac acaaagttcg
     7261 cgctatggtt ttgaaggtcg agaagcccca cctgaaatag caaatcttta tcttggaaaa
     7321 aaaataccag atgaattaag aaaaaaagga gctatgtccc cggtccgtta ctcacctaat
     7381 ttttgagtct ttaagtgata agcataaacc gcagcacgtc atgcatacgt cgtgtctgcg
     7441 gtttttcttt tttgcttaca cggtgtctgg ttcttctggc cactcaatat caggtgcagt
     7501 tgatgtatca acacggttca gcaacacccg atactttttc catgcggcca gtaacgcttt
     7561 ctcttcctcc gttgcgattt ccagctcaac aacagtctga acgtaccggg aacagcctcc
     7621 ttcagagctt gaaggatatc aatgttcgct tcctgttaac tgccggacaa gtgcaaccag
     7681 ttcgcttacc tgattttcca gagtgctgat ccgggtgtct gctgttgcca gattttcacg
     7741 tagcgttgtg ttttcctctt ccagcgcggt aacgcgatca tctgtttcac gggcgaactg
     7801 aacaagtaag cccgtcacgg cggcgtagtc aacattcaga taacgtgttt cttcacgtaa
     7861 ttcattgccg tcaaccgttg gtccctgcaa ctcttcaccg taatgagtaa acgatcccac
     7921 agcttctggt attgcctcca ttacttcctg tgcaataacg ccagcataag gcatcccgtt
     7981 ttccctgagc gtgtaggtgt atccgttcat tttacggatt gctttcgtcg cgtcgctgat
     8041 aacgagaata tcgtctttaa ggtcgcggtc tgatgactga ttcagcgttg tgcaattaat
     8101 agcgccattt acatcaaaca actggcctgc tgacgttttt tgcgcataaa acagatacgc
     8161 agcagacgtg ccaacctcaa aaacgttttg tcgagtactg gaaccccaca ccctgatacc
     8221 aactggtagt tctgaattac ctgaattaag taaagcaaaa cgattgccag tccctgtttg
     8281 ttttctaatt actaaatcgg cagttgagtt aacctcatct ttgttgatgg ttagcgcctg
     8341 tgctgtagca ccgttgacag taccgcttag tagttgcacc gcgccatcat cgccatttaa
     8401 cagcacttga gcgctgcttc tgtggttttt aaggcacaac atttttcccg tgtttttcga
     8461 tgtacctact gaccacgccg aattggttcc agtgctatca acaccgcgca cagcacaatt
     8521 catactactg taatctgacg tactccccag cacatcaaca cgcccgccgc ctaattttga
     8581 tggagtcgtc gaggttaacg acctgacggt tacatcctga ttccatcctg aaacaactac
     8641 ctgactccac tccgtccaac ttccattgac aacaaagcga acgtatatgc gttttgtgtc
     8701 gctgccaatc agcgtttgca tgatcgcata atctgaaccc gtagttttac gagtagattc
     8761 tacacgtagc aaaaagttac cgcttacgcc gtcaggctta ttggtaatat atgcaccacc
     8821 tgcgacagtt ttgcattgat agtatttaac agatccagcc actgtattag caatgatcaa
     8881 gtcgtttaaa tcaatagtct tgttgtcaat ggtttccggc tcgatagatc ccgccatacc
     8941 tgcgttaaat gtggcaaggt cagaaaaggt tgttttaccg cctaccgtca ccccgctatc
     9001 gagttttgct tgtgtgatta ctttgctgtt ttttgtgtgg tcagaataac taccaaaata
     9061 caaatcaccg tcaccagcta tacctaggta tttttgatca acatcatcaa ccttaaaccc
     9121 aatggacaag tttttaattg tgttgttgct cgttaaaacc aaaggcgtat gctgagatcc
     9181 tttgatgctt accgtagtgt tatcggaact ggtgcttgtg tttgcaaatt ctgcggtggc
     9241 tgcgctgatt ttgtaagtta tttttagatt gcgggcctca accctcccgt cgtggcgaac
     9301 aataaaatca ccacttgatt caccttttgt gtttttcgcc ctgatgtgga tttcgccaag
     9361 tgattcgttg ttttcaggcg accaaacaac gccgcgctcg cttccgtcgt tgttcatgaa
     9421 ccacagatga gcggttcctt ttgctgattt tagcctgata gatggcgttt ctttcaatat
     9481 atcaagatca ccagacataa catcgccgga ttttttcacc tgcgcatcgt tcgttacgtt
     9541 gccaagtcca acatccgatt tcgacggctt gtttgccgag ccgtatagct cattgacgct
     9601 aaaagtcccg ttgttaagcc gggcatcgtt gccggcatag atcttaatct tcgcggttga
     9661 gtagtcgata ttgatcgctg accacgtatc gccgcaccgc gagaatatac cagaaccgtg
     9721 gctataaatg gtcgccgtgc ttccgggcgg cttatcaccg cgccagaaat gaccaccttt
     9781 gtcacgcaga gcttttaaga tctctacatc gctcatcttg ccgtttgttg ctatcccgct
     9841 accgccaaga ccgaaagcgc ctgtaagcat agcattagaa agacccaaat ctgctttagt
     9901 cggcttgttt aactggtcgt aaatccttac aacggggctt tcaacatatc cagaaggcgc
     9961 ggcggtttgc ttaacaaatc cgtctgggat gtataattct gtgcgtgccg tctgcgccag
    10021 taccgcaacc tttgcgccgt tgataaatgc gcgctggaag gcccaaacct caataaagcc
    10081 gtcaccttta accaggccgt aacgcatctg gttgttatcg gtcagcccgg ttgaccctaa
    10141 gcggcgtata ctcagatgac gagaaacatt atccgcagta agcgaagaag gcagaccgcg
    10201 cgccgagatc tcgataaagt caatgtttcc gtaaggggag ccgtagttac cgccgttagt
    10261 aaccattagc gttacatggc aattgttgct tccgggatcg gaaagtttcg cgatctttat
    10321 gtaaaaaggc tcactaccag taacaacggg ccaatcgtat tgcgtcatcg ggctgacgag
    10381 tcccccgacc ttgtcgagat attctttcgc cttgttctct gacgctttag cgttggtttc
    10441 gctgaccttt gctgctgcct cactattttt cgcgttggtt tctgattttt tggctg
//
LOCUS       DAJOSY010000134        10323 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJOSY010000134 DAJOSY010000000
VERSION     DAJOSY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.134"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            114..689
                     /gene="ssuE"
                     /locus_tag="OHN27_003814"
     CDS             114..689
                     /gene="ssuE"
                     /locus_tag="OHN27_003814"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCQ2288179.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            682..1641
                     /gene="ssuA"
                     /locus_tag="OHN27_003815"
     CDS             682..1641
                     /gene="ssuA"
                     /locus_tag="OHN27_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCQ2288180.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPESKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1638..2783
                     /gene="ssuD"
                     /locus_tag="OHN27_003816"
     CDS             1638..2783
                     /gene="ssuD"
                     /locus_tag="OHN27_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCQ2288181.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLQGETVDFNGKHIHVRGA
                     KLLFPAIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPLLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2794..3585
                     /gene="ssuC"
                     /locus_tag="OHN27_003817"
     CDS             2794..3585
                     /gene="ssuC"
                     /locus_tag="OHN27_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCQ2288182.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3582..4349
                     /gene="ssuB"
                     /locus_tag="OHN27_003818"
     CDS             3582..4349
                     /gene="ssuB"
                     /locus_tag="OHN27_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCQ2288183.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4392..7004)
                     /gene="pepN"
                     /locus_tag="OHN27_003819"
     CDS             complement(4392..7004)
                     /gene="pepN"
                     /locus_tag="OHN27_003819"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCQ2288184.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7270..8472
                     /gene="pncB"
                     /locus_tag="OHN27_003820"
     CDS             7270..8472
                     /gene="pncB"
                     /locus_tag="OHN27_003820"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCQ2288185.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8641..10041
                     /gene="asnS"
                     /locus_tag="OHN27_003821"
     CDS             8641..10041
                     /gene="asnS"
                     /locus_tag="OHN27_003821"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCQ2288186.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2322 a         2647 c         2791 g         2563 t
ORIGIN      
        1 ttcagaaaaa gatacacaaa atttatattt ggaattttct tgtctctccg acagactgac
       61 cgaattgtta tcaatgttaa caaaaaaaga acaattggtt ataaggagag agtatgcgtg
      121 tcatcaccct ggcgggtagt cctcgctttc cttctcgctc cagctccttg ctggaatatg
      181 cgcgggaaaa actaaatggc ctggatgtag aggtttatca ctggaatctg caaaacttcg
      241 ccccggaaga tctactttat gctcgtttcg atagtccggc actcaagacc ttcaccgaac
      301 agctgcaaca ggccgatggg ctgattgtcg ccacgcctgt gtataaagcc gcctattccg
      361 gtgcgttgaa aaccctgctc gacctgctgc cagaacgcgc tttgcaaggc aaagtggtgc
      421 taccgctggc gacgggcggt accgtggccc atctgctggc ggtcgattat gcccttaaac
      481 cagttttaag cgcactgaaa gctcaggaga tcctgcacgg cgtgtttgcc gatgactcac
      541 aagtaattga ttaccatcac agaccccagt tcacgccaaa tctgcaaacc cgtcttgata
      601 ccgcgctaga aactttctgg caggcattgc accgccgcga tgttcaggtt cctgaccttc
      661 tgtctctgcg aggtaatgcc catgcgtaac atcattaaac tggcgctggc gggattgctt
      721 agcgtctcta cttttgcggt tgctgcagaa tcctcgcctg aagcgttacg tataggctat
      781 cagaaaggca gtattggtat ggtgctggca aaaagccacc agttactgga aaaacgctat
      841 ccggaatcaa aaatctcctg ggtggagttc cccgcgggtc cgcaaatgtt ggaagcgtta
      901 aacgttggca gtattgatct cggcagtacc ggggatattc cgccaatctt tgcccaggct
      961 gccggggctg atttggtgta cgtgggcgtc gagccaccga agcccaaagc cgaagtgatt
     1021 ctggtggcag aaaacagccc gatcaaaacc gtagccgatc ttaaaggtca caaagttgcc
     1081 tttcagaaag gttccagttc acacaacctt ttactgcgtg cactgcgtca ggccggactt
     1141 aagtttaccg atatccaacc cacttacctg acgcccgctg atgcccgcgc cgcgttccag
     1201 caaggtaacg ttgacgcctg ggctatctgg gatccctact actctgctgc attattacag
     1261 ggcggcgtgc gggtgctgaa agacggcacc gatctcaatc aaactggatc gttttatctg
     1321 gcagctcgtc cctatgcaga aaaaaacggc gcttttattc agggcgtact ggcaaccttt
     1381 agtgaggccg atgcgttaac ccgcagccag cgcgagcaaa gcatcgcttt actggcaaaa
     1441 acgatgggct taccggcacc ggtgattgcc tcttacttag atcatcgccc tcctaccacc
     1501 atcaaaccgg ttaacgccga ggttgccgcc ttacagcagc aaacggcaga tctgttttat
     1561 gaaaatcgtc tggtgccgaa aaaagtcgat attcgccagc gcatctggca gcccactcaa
     1621 ctggaaggaa aacaattatg agtctgaata tgttctggtt tttaccgacc cacggtgacg
     1681 ggcattatct gggaacggaa gaaggttcac gcccggttga tcacggttat ctgcaacaaa
     1741 ttgcgcaagc ggcggatcgt cttggctata ccggtgtgct aattccaacg gggcgctcct
     1801 gcgaagatgc gtggctggtt gccgcatcga tgatcccggt gacgcagcgg ctgaagtttc
     1861 ttgtcgccct gcgtcccagc gtaacctcac ctaccgttgc cgcccgccag gccgccacgc
     1921 ttgaccgtct ctcaaatgga cgtgcgttgt ttaacctggt cacaggcagc gatccacaag
     1981 agctggcagg cgacggagtg ttccttgatc atagcgagcg ctacgaagcc tcggcggaat
     2041 ttacccaggt ctggcggcgt ttattgcagg gagaaaccgt cgatttcaac ggtaaacata
     2101 ttcatgtgcg cggagcaaaa ctgctcttcc cggcgattca acagccgtat ccgccacttt
     2161 actttggcgg atcgtcagat gtcgcccagg agctggcggc agaacaggtt gatctctacc
     2221 tcacctgggg cgaaccgccg gaactggtta aagagaaaat cgaacaagtg cgggcgaaag
     2281 ctgccgcgca tggacgcaaa attcgtttcg gtattcgtct gcatgtgatt gttcgtgaaa
     2341 ctaacgacga agcgtggcag gccgccgagc ggttaatctc gcatcttgat gatgaaacta
     2401 tcgccaaagc acaggccgca ttcgcccgga cggattccgt agggcaacag cgaatggcgg
     2461 cgttacataa cggcaagcgc gacaatctgg agatcagccc caatttatgg gcgggcgttg
     2521 gcttagtgcg cggcggtgcc gggacggcgc tggtgggcga tggtcctacg gtcgctgcgc
     2581 gaatcaacga atatgccgcg cttggcatcg acagttttgt gctttcgggc tatccgcatc
     2641 tggaagaagc gtatcgggtt ggcgagttgc tgttcccgct tctggatgtc gccatcccgg
     2701 aaattcccca gccgcagccg ctgaatccgc aaggcgaagc ggtggcgaat gattttatcc
     2761 cccgtaaagt cgcgcaaagc taaggaagaa ataatggcaa cgccagtgaa gaagtggtta
     2821 ttgcgcgttg ccccctggtt tttaccggtg ggcatcgtgg cggtgtggca actggcctcc
     2881 tcggttggct ggctgtcgac gcgtattttg ccttcaccgg aaggagtggt gacggcgttc
     2941 tggacgctct ccgccagcgg cgaactgtgg cagcatctgg cgatcagctc ctggcgggcg
     3001 ctgattggct tttcaattgg cggatcgctg gggttgattt tgggactgat tagcgggcta
     3061 tctcgctggg gagaacggct gctggatacc tcaattcaga tgttgcgcaa cgtgccgcat
     3121 ctggcgctga tcccgctggt gattttgtgg tttggcattg atgaatccgc aaaaatcttt
     3181 ctggtggcgc tcggtacgct attccccatt tatatcaaca cctggcatgg catccgtaat
     3241 atcgatcgcg ggttggtgga gatggcgcgt agctatggat tatccggcat accgctgttt
     3301 atccatgtga tcctgcctgg tgccctgccc tcaattatgg tcggagtgcg ttttgcgttg
     3361 ggcctgatgt ggctgacgct gattgttgcc gaaaccattt ctgccaattc cggcattggt
     3421 tatctggcga tgaatgcgcg ggagtttttg caaacggacg tggtggtagt cgctattatt
     3481 ctttacgccc tgctcggcaa actggctgac gtcagcgcgc agctgttaga gcgcctctgg
     3541 ttgcgctgga acccggctta tcatttgaag gaggccacgg tatgaatact gctcgtctga
     3601 accagggcac accattgttg ctcaatgcag taagcaaaca ttacgcggaa aatatcgtcc
     3661 tgaaccaact ggatttacat attccggcag gtcagtttgt ggcggtggtg ggccgcagcg
     3721 gtggtggcaa aagtaccctg ctgcgcctgc tggcaggtct ggaaacgcca accgcaggcg
     3781 atgtgttagc gggcaccaca ccgctggctg aaattcagga agacacgcga atgatgtttc
     3841 aggatgcgcg tctgctaccg tggaaatcgg tgattgataa cgttgggtta ggccttaaag
     3901 gccagtggcg cgatgccgca cgtcgagcgt tggctgcggt aggactggag aatcgcgccg
     3961 gggaatggcc tgccgcactt tctggcgggc agaaacagcg agtggcgctg gcaagagcgt
     4021 tgattcatcg accgggttta ttgttacttg atgaaccgct cggtgcgctg gatgccttaa
     4081 cgcgactcga gatgcaggat ttgattgtgt cactttggca ggagcacggc tttaccgtac
     4141 tgttggtgac gcatgatgtg agcgaagcgg tagcaatggc tgaccgggtg ttgttaattg
     4201 aagagggaaa aattggtctg gatttgacgg tggatatccc ccgtccgcgc cggttagggt
     4261 cggtgcgatt agcggagctg gaagcggaag tgttgcaacg ggtaatgcag cgtggtgagt
     4321 ctgaaactcg cctgagaaaa caggggtaaa atccccgaat ggcggcgcta ttgccgccat
     4381 tcggttattt attaagccag tgctttggtg atcttctcgt acagatcgcc agagagattt
     4441 tccagccctt tcaactgttc cagcgccgcg cgcattttct cctgacgttt ggcatcataa
     4501 cgtttcaggc gaatcagcgg ctcaatcagg cgcgaagcca cctgcgggtt acggctgttg
     4561 aggtcggtaa gcatttccac caggaactgg taaccgctgc catcttcggc atggaacgct
     4621 gccgggttgc tgcccgcaaa cgcaccaatc aacgaacgaa tacggttcgg gttgctcatg
     4681 gtaaatgagc gatgctgcaa caggccgcgc accgtctcca gcacattcgc cgccgggctg
     4741 gtggcttgca ggataaacca tttatccatc accagaccgt cctgatgcca cttgtcgtcg
     4801 tactcctgca tcagcgcgtc acggcatggc agctgtgcag caaccgccgc agaaagcgct
     4861 gccagcgcat cggtcatatt gtttgcttca tggtattgct tgctcaccag cacgtcagcc
     4921 agatgcgttt caccaaaagc aaggaagcgc agacaggcat tacgcagagt gcgttttgca
     4981 atatcttcat gctcaacgcg gtactcgctc tggtaattcg cgttgtaaat agccagcagc
     5041 tcatccgcca gttcagtcgc cagagtacga gtgagtgctt cgcgtacttc ggcaatagca
     5101 atcgggtcga tgatatcgaa cagttcagcc atttcattga cggatggcag agtcagaatt
     5161 tctgccgcca gtgccgggtc aatcttctca tcgagcagca ccgcgcggaa ggcgtcggcc
     5221 acatgcaccg gcagagacag cggctgccct tgctgatgac gggcgacgtt cagcttgatg
     5281 taggttgcca gcaaactttg cgccgcatcc caacgggaga aatcattacg cgcatgacgc
     5341 atcaggaagg tcagttgctg atcgctccac ttatattcca gtttcactgg cgcagagaat
     5401 tcgcacagca gcgcaggcac cggctggaag tagacattat caaagacaaa ggtctgttcc
     5461 gcctgagtga cgttcagcac ggaattcacc ggatgaccgc ctttctgcaa cgggatcact
     5521 ttgccttcgt tatcatacag ttcgatggca aacggaatat gcagcggctg tttttctgcc
     5581 tgatccggcg tggctggcgt gcgctggctg atggtcaggg tgtactgctc ggtttccgga
     5641 ttgtagtcgt ctttgacggt cacaatcggt gtaccggact ggctgtacca acggcggaaa
     5701 tgggagagat cgacattcga cgcatcttcc atcgcctgca caaagtcgtc acaggtcgct
     5761 gcactaccat catgacgctc gaaataaagc tgcatccctt tctggaagtt ttcttcgcca
     5821 agcagggtgt ggatcatgcg aatcacttcc gcgcccttct cgtaaacggt cagggtgtag
     5881 aagttgttca tctcaatgac catatccggg cggatcgggt gcgccatcgg gctggcgtct
     5941 tctgcaaact gcaatccgcg catggtgcgt acattattga tgcggttaac tgcgcgggaa
     6001 ccaaggtcag agctgaactc ctgatcgcgg aagacggtta aaccttcttt caggctgagc
     6061 tggaaccagt cgcgacaggt cactcggtta ccggtccagt tatggaaata ttcatggccg
     6121 ataacgcgtt caatatcgag gtaatctttg tcggtggcgg tgtcggtgcg ggccagcaca
     6181 tatttggagt taaagatatt cagcccctta ttctccattg cgcccatatt gaagaaatcc
     6241 accgcgacga tcatatagat gtcgaggtca tactccaggc caaagcgttc ttcatcccat
     6301 ttcatggagt ttttcagcga ggtcatcgcc cacggcgcgc gatcaaggtt gccgcgatcg
     6361 acgtacagct ccagtgctac ttcgcgacca gaacgcgtgg taaaggtatc gcgcagtaca
     6421 tcaaagtcgc ctgccaccag cgcaaatagg tagcacggtt tcgggaacgg gtcctgccac
     6481 tgtacccaat ggcgtccgtt ttccagttcg ccttgcgcaa cgcggttacc attggaaagc
     6541 aggaagggat atttgatttt atcggcaata attttggtgg taaaacgcgc cagcacgtcc
     6601 gggcggtcga gataatacgt aatatggcgg aaaccttcgg cttcacactg ggtgcaaagc
     6661 gcatcgcctg actgataaag cccttccagc gcggtattcg ccgccgggct tatttcatta
     6721 atgatcttaa gcgtaaaacg ctccggcaaa ttactgataa ccagtgcgcc ctcttcttct
     6781 ttccaggcgg tccacggctc atcattaata tgaacagaaa ccagtttgag gtcttcgccg
     6841 ttgagacgaa ggggagcatc tgatgcacca tgacggacag cctggctgac cgcggtaacg
     6901 accgtctttt gcgcgtcgag gtcaaaggtc aagtcaatat cagtaatctg gtaatccggc
     6961 gcacgataat cgtgacggta tttggcttgt ggctgttgag tcataaataa cctttagcat
     7021 cttttataga gtctggtgtt cagtctattc ctgttgcgta aatcgcgcta tgcagaatct
     7081 tcatcttttc aggtacaaac gcctttattg ctacattttt ataacataca ccgcgtaatg
     7141 ccatcgacca gaaaggtggc atatggtgtg atcggggttc aataaattgc gaaacaaggt
     7201 atactccagc agttcctgaa gatgtttatt gtactaaacg ctcctgtacg aggacgctac
     7261 tgcgcaccta tgacacaatt cgcttctcct gttctgcact cgttgctgga tacagatgct
     7321 tataagttgc atatgcagca agccgtgttt catcactatt acgatgtgca tgtcgcggcg
     7381 gagtttcgtt gccgaggtga cgatctgctg ggtatttatg ccgatgctat tcgtgaacag
     7441 gttcaggcga tgcagcacct gcgcctgcag gatgatgaat atcagtggct ttctgccctg
     7501 cctttcttta aggccgacta tcttaactgg ttacgcgagt tccgctttaa cccggaacaa
     7561 gtcaccgtgt ccaacgataa tggcaagctg gatattcgtt taagcggccc gtggcgtgaa
     7621 gtcatcctct gggaagttcc tttgctggcg gttatcagtg aaatggtaca tcgctatcgc
     7681 tcaccgcagg ccgacgttgc gcaagccctc gacacgctgg aaagcaaatt agtcgacttc
     7741 tcggcgttaa ccgccggtct tgatatgtcg cgcttccatc tgatggattt tggcacccgt
     7801 cgccgttttt ctcgcgaagt acaagaaacc atcgttaagc gtctgcaaca ggaatcctgg
     7861 tttgtgggca ccagcaacta cgatctggcg cgtcggcttt ccctcacgcc gatgggaaca
     7921 caggcacacg aatggttcca ggcacatcag caaatcagcc cggatctagc caacagccag
     7981 cgagctgcac ttgctgcctg gctggaagag tatcccgacc aacttggcat tgcattaacc
     8041 gactgcatca ctatggatgc tttcctgcgt gatttcggtg tcgagttcgc tagtcggtat
     8101 cagggcctgc gtcatgactc tggcgacccg gttgaatggg gtgaaaaagc cattgcacat
     8161 tatgaaaagc tgggaattga tccacagagt aaaacgctgg ttttctctga caatctggat
     8221 ttacgcaaag cggttgagct ataccgccac ttctcttccc gcgtgcaatt aagttttggt
     8281 attgggactc gcctgacctg cgatatcccc caggtaaaac ccctgaatat tgtgattaag
     8341 ttggtagagt gtaacggtaa accggtggcg aaactttctg acagccctgg caaaactatc
     8401 tgccacgata aagcgtttgt tcgggcgctg cgcaaagcgt tcgaccttcc gcatattaaa
     8461 aaagccagtt aatatcatca gggagctaat cggctccctt tttttacctt taattccgaa
     8521 atctttcgct gcatttgcga attctgcttg tctgattgca gatgccaggt aacataggta
     8581 tccccccatt tacgggtgga caagtgttta ttttttccga ctattaacag agagaatatt
     8641 atgagcgttg tgcctgtagc cgacgtactc cagggccgcg tagccgttga cagcgaagtc
     8701 accgtacgcg gatgggtacg tacccgccga gattcaaaag ctggcatctc cttcctcgcc
     8761 gtttatgacg gttcctgctt tgatcctgta caggctgtca tcaataattc tctgcccaat
     8821 tacaatgaag acgtcctgcg tctgaccacc ggttgctcgg tcattgtgac gggtaaagtc
     8881 gtggcgtcgc cgggccaggg gcaacaattt gaaattcagg ccagcaaggt tgaagttgct
     8941 ggttgggttg aagatccaga cacttacccg atggcggcaa aacgccacag cattgagtat
     9001 ctgcgtgaag tcgctcacct gcgtccgcgc acaaacctga ttggtgccgt cgcgcgcgtt
     9061 cgccatacgc tggcgcaggc gctgcatcgc ttctttaacg agcagggatt cttctgggtt
     9121 tcaacgccac tgattactgc gtctgatacc gaaggggctg gcgaaatgtt ccgcgtttct
     9181 acgctggatc tggaaaatct gccgcgtaac gatcagggca aagtggattt cgacaaagac
     9241 ttctttggta aagagtcttt cctgaccgta tctggccagt tgaacggcga aacctacgct
     9301 tgcgcattat ccaaaattta taccttcggc ccgactttcc gtgctgaaaa ctccaacacc
     9361 agccgtcacc tggcggagtt ctggatgctg gagccggaag tggcgtttgc taacctgaac
     9421 gatatcgcgg gtctggctga agccatgctg aaatatgtct tcaaagcggt tctcgaagaa
     9481 cgcgcagacg acatgaaatt cttcgctgaa cgcgtagata aagatgccgt ttcacgtctg
     9541 gaacgcttca ttgaggccga tttcgcgcag gtggattaca ccgacgcagt gaccattctc
     9601 gaaaactgcg gcaggaagtt tgaaaacccg gtttactggg gcgtcgatct ctcttctgag
     9661 catgagcgtt atctggcgga agaacacttt aaagcaccgg tagtggttaa aaactatccg
     9721 aaagatatta aagcgttcta tatgcgcctc aacgaagacg gtaaaaccgt tgcggctatg
     9781 gacgttctgg ctccgggcat cggtgagatc attggtggct cccagcgtga agaacgtctg
     9841 gacgttctgg acgagcgtat gctggaaatg ggcctgaata aagaagatta ctggtggtat
     9901 cgcgatctgc gtcgctacgg tactgttccg cattcaggtt tcggtcttgg ttttgaacgc
     9961 ctgattgctt acgtaactgg cgtgcaaaac gtgcgtgatg tgattccgtt cccacgtact
    10021 ccgcgcaacg ccagcttctg attttcttcc tcaaagagcc agcgcctgct ggctcttttt
    10081 tatcctttct gtcaagttat ctgtttgtta agtcaagcaa tctatttgca accccgccat
    10141 aacgatcatc ctgttacgga atattacatt gcaacattta cgcacaaaaa ataacctgga
    10201 ttcttattgc ggattagttt tttcttagct gatagcacaa ttttcatact attttttggc
    10261 attctggatg tctgaacgag gatttagtgc caggtcgata aagtttccat cagaaacaaa
    10321 att
//
LOCUS       DAJOSY010000135        10216 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJOSY010000135 DAJOSY010000000
VERSION     DAJOSY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.135"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(114..2687)
                     /gene="clpB"
                     /locus_tag="OHN27_003822"
     CDS             complement(114..2687)
                     /gene="clpB"
                     /locus_tag="OHN27_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCQ2288187.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2817..3548)
                     /gene="pgeF"
                     /locus_tag="OHN27_003823"
     CDS             complement(2817..3548)
                     /gene="pgeF"
                     /locus_tag="OHN27_003823"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCQ2288188.1"
                     /translation="MSKLIVPQWPQPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGGEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="OHN27_003824"
     CDS             complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="OHN27_003824"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCQ2288189.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIIINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4660..5397
                     /gene="bamD"
                     /locus_tag="OHN27_003825"
     CDS             4660..5397
                     /gene="bamD"
                     /locus_tag="OHN27_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCQ2288190.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5668..6009
                     /gene="raiA"
                     /locus_tag="OHN27_003826"
     CDS             5668..6009
                     /gene="raiA"
                     /locus_tag="OHN27_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCQ2288191.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6112..6237
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6113..6160
                     /gene="pheL"
                     /locus_tag="OHN27_003827"
     CDS             6113..6160
                     /gene="pheL"
                     /locus_tag="OHN27_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCQ2288192.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            6259..7419
                     /gene="pheA"
                     /locus_tag="OHN27_003828"
     CDS             6259..7419
                     /gene="pheA"
                     /locus_tag="OHN27_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCQ2288193.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="OHN27_003829"
     CDS             complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="OHN27_003829"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCQ2288194.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="OHN27_003830"
     CDS             complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="OHN27_003830"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCQ2288195.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKSI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9874..>10216
                     /locus_tag="OHN27_003831"
     CDS             9874..>10216
                     /locus_tag="OHN27_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCQ2288196.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADEN"
BASE COUNT         2607 a         2717 c         2394 g         2498 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca cttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat catttactgg
      121 acggcgacaa tccggtcttc attaacttcc aggcgaatca ctttacccgg aaccaattca
      181 ccagacagta tttgctgtgc cagcgggttt tcgatctgct gctgaattgc acgtttcaga
      241 ggacgtgcac catagaccgg atcgtaaccg ttctcgctca gcagtttcag cgcctcgtca
      301 gaaatgtgga tttcataacc acgttcttcc agacgtttgt acagacgttt caactgaatc
      361 tgcgcaatcg aggcaatgtg ctgttcaccc agcggatgga agaccaccac ttcatcgata
      421 cggttaatga attccggacg gaagttatgg cttaccacac cgagcaccag ctctttcatg
      481 tgcgcataat ccagttcacc gaagcgttcc tgaatcagat cggaaccgag gttagaggtc
      541 ataatgacga ccgtattacg gaagtcgacc gttctccctt gcccgtcagt cagacgccca
      601 tcatccagta cctgcaacag aatgttgaag acatccggat gcgctttttc cacttcatcc
      661 agcaggatga cggaatacgg acgacgacgc accgcttcgg tcaggtagcc accttcttca
      721 taaccgacat atcccggagg cgcaccaacc aaacgagaca ccgagtgttt ctccataaac
      781 tcggacatat cgatacggac catcgcctcg tcgctatcaa acataaagtt cgccagcgcc
      841 ttacaaagct ctgttttccc cacaccagtt gggccgagga acaggaatga accaatcggg
      901 cgatttggat ccgccagccc cgcacggcta cgacgaatag cgttagatac cgcatcaacc
      961 gcttcgttct gaccaattac gcgatggtgc agttcttgct ccatacgcag cagtttttcg
     1021 cgctcgcttt ccatcatgcg agaaaccgga atccccgtcc aacgcgccag cacttcagca
     1081 atttcggcgt cggtcacttt attacgcaac agacgcatag ttttgccttc gagctgcgtt
     1141 gcggcttcca gttgcttttc cagttccggg attttgccgt attgcagttc agacatccgc
     1201 gccaggtccc ccacacggcg agcctgttca atagcgattt tcgcctgttc cagttccgct
     1261 ttaatggtct gcgtaccaga aagcgatgcc ttctctgctt tccactcttc ttctaactcg
     1321 gagtactgac gttctttgtc gctcagttct tcgttgagca tatccagacg ttttttactg
     1381 gcttcatcag actctttcat taacgcctgt tgttccagtt tgagctggat gatacgacga
     1441 tcgagtcggt cgagttcttc tggttttgag tcaatctgca tacgaatgct ggatgctgct
     1501 tcatcgatca ggtcgatggc tttatccggc agctgacggt cagcaatgta gcgatgagac
     1561 aacgtcgccg ctgcaacaat tgccgggtca gtaatttgca catggtggtg caattcgtaa
     1621 cgttctttca ggccacgcag aatcgcaatg gtatcttcaa cagaaggctc ggcaacaaac
     1681 actttctgga aacgacgttc cagcgcagca tctttttcaa tgtactggcg atattcgtca
     1741 agcgtcgtgg cacctacgca gtgcaattca ccacgcgcca gcgccggttt cagcatgttt
     1801 ccggcgtcca ttgcgccatc ggctttaccc gcgccgacca tggtatgtaa ttcgtcgata
     1861 aataggatga cgttgccttc ctgtttggca agatcgttaa gcacgccttt taaacgttct
     1921 tcaaactcac cgcgatattt cgccccagcc accagcgcgc ccatatccag cgccagtacc
     1981 cggcggcctt tcaacccttc cggcacttcg ccgttgataa tacgctgcgc cagaccttca
     2041 acgatggcag ttttaccgac gccgggttca ccaatcagta ccgggttatt tttagtacga
     2101 cgttgcagca cctgaatggt acggcgaatt tcttcatcac gaccaatcac cggatcgagt
     2161 ttgccctgtt cggctcgttc ggtaaggtcg atggtatatt ttttcaaagc ctgacgttgg
     2221 tcttcagcac cttgatcgtt cacgctttca cctccacgca tttgttcaat cgcttgagta
     2281 atgttggcgg tggtcgcccc tgctgctttc aggatgtcgg ccagcgtgcc gcgagactca
     2341 agtgccgcca gaacgaacag ttctgacgag ataaagttat caccacgttt ttgcgccagc
     2401 ttgtcgcaaa gattaagaac gcgcaccaga tcctgtgatg gctggacatc accaccagta
     2461 ccttcaacct gcggtaaacg atttaatgcc tgattgatat ctgtgcgcaa ctggccagca
     2521 tttatgccag cggatgttaa taaaggacta accgaacccc cttcctgatt cagcagggcg
     2581 ctcattaaat gaagtggttc gataaattgg ttgtcgtgcc cgagtgcaag tgattgggca
     2641 tcggcaagag caagctggaa tttattagta agacgatcca gacgcataac tcctcccata
     2701 acggatcaaa gttgctactg gagattaaat gaggtcatcc ctcaattatt caaggttatt
     2761 gaccagatta atgtgaaaag aaaatcacgc gtaccggatc gtcttgattc tttaggttat
     2821 atcagccaaa tgaaacttgc catacgaccg gtggtcttgt cgcgacgata agagaagaaa
     2881 gtctcatttt ccgtatatgt acaacggtcg ccgccgaaaa tttgctcaac acccacgttc
     2941 gccagacgct gccgggcaag ctgataaata tccgccagat acttatcacc atgctgaatg
     3001 aaagctgcac ttgctttagc gtctactgcc ataaacgcct cgcgaacctc cccccccact
     3061 tcgaacgcgc gtggaccaat tgccggccct aaccaggcga gaatattttc cggattatca
     3121 gcaaaacagg aaaccgtctc ttccagcacg cctgcgcaca gtccacgcca gccagcatga
     3181 gcggcggcga cttccgttcc cgctcgattg caaaacagca cagggaggca gtcggcagtc
     3241 atcactgcgc aaaccgtgcc gggcgtattg ctataagagg catccgcccg ttttgaggca
     3301 taaggttcgc cagtgagctt aagcacatct ttgccgtgta cctgctcaag ccagaccggt
     3361 ttagaaggca aattgcccgc agcaaaaagt cgcttgcgat tctcctcaac gtgatccggg
     3421 ttatcgccac aatgggcacc gaggttgagt gagtcatacg ggggcaagct cacgccgccg
     3481 atacgagtgg agctacaggc cgcaacacct tttggctgcg gccactgcgg gacaatcagc
     3541 ttactcataa ccagtccact tcatccttat gttcttcgaa atcggcgcgc atcacctcaa
     3601 tcagctccac catatcttgt ggaataggcg catgccattc catttcgatg ccggagatcg
     3661 ggtgataaag acgcagcatg gttgcatgta gcgcctggcg gtcaaactta cgcagcgtgg
     3721 agataaatgc ttccgaagca ccttttggcg gacgcggacg gccaccataa accggatcgc
     3781 ccaccagcgg atgagtgata tgggccatat gcacgcggat ctggtgcgta cgtccagttt
     3841 ccagacgcaa ccgcagacgc gtgtgcacac ggaagtgttc catgatgcga tagtgagtca
     3901 ccgctggttt gcccatcgga tgcaccgcca tatgggtacg tttggtcggg tggcgactga
     3961 ttggctcgtc caccgtgcca cctgcggtca tatgaccaat cgccaccgct tcatactcac
     4021 gagtaatttc acgccgttgc aaagattcga ctaaacgcgt ctgagccgga acggtttttg
     4081 ccacaaccat caggccagtg gtgtctttat ccagacgatg gacgatgccc gcacgcggta
     4141 catcggcaat gggtggatag taatgaagca acgcattcag taccgtgcca tccgggttac
     4201 ccgcgccagg atgtaccacc aggtcgcgcg gtttattaat gataataatg tcttcatctt
     4261 catagacgat atccagcggg atatcctgcg gttcaaaacg cgcttcttct tcaatctcag
     4321 cgttgatggc aacctgctcg ccacccaata ctttttcttt cggcttatca caaactttgc
     4381 cgttaaccag cactcgctgg tcgaggatcc attcttttat tcgcgaacgt gaataatccg
     4441 ggaacatttc ggccaaagcc tgatctaagc gttgaccgag ttggttttcg gacaccgttg
     4501 cagtgagctg tactcgttgt gccatatata ctgcttcttc gtttatcgtt ggggttttac
     4561 ggctttgccg tttaatatag tgtgctattg tagctggtct taaccgggag caggaacaga
     4621 gaatctcccg tattacattt tgaggaaagt caaaacgtca tgacgcgcat gaaatatctg
     4681 gtggcagccg ccacactaag cctgtttttg gcgggttgct cggggtcaaa ggaagaagta
     4741 cctgataatc cgccaaatga aatttacgcg actgcacaac aaaagctgca ggacggtaac
     4801 tggagacagg caataacgca actggaagcg ttagataatc gctatccgtt tggtccgtat
     4861 tcgcagcagg tgcagctgga tctcatctac gcctactata aaaacgccga tttgccgtta
     4921 gcacaggctg ccatcgatcg ttttattcgc cttaacccga cccatccgaa tatcgattat
     4981 gtcatgtaca tgcgtggcct gaccaatatg gcgctggatg acagtgcgct gcaagggttc
     5041 tttggcgtcg atcgtagcga tcgcgatcct caacatgcac gagctgcgtt tagtgacttt
     5101 tccaaactgg tgcgcggcta tccgaacagt cagtacacca ccgatgccac caaacgtctg
     5161 gtattcctga aagatcgtct ggcgaaatat gaatactccg tggccgagta ctatacagaa
     5221 cgtggcgcat gggttgccgt cgttaaccgc gtagaaggca tgttgcgcga ctacccggat
     5281 acccaggcta cgcgtgatgc gctgccgctg atggaaaatg cataccgtca gatgcagatg
     5341 aatgcgcaag ctgaaaaagt agcgaaaatc atcgccgcaa acagcagcaa tacataacag
     5401 aaacctgaaa cacaaaacgg cagcccttga gctgccgttt ttttattctg tcagttgtga
     5461 aactgaagcg atttagtcgc tatcgatctc atcaaatatg gctcgctttg agatattcct
     5521 caagtaaaaa aacatctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg
     5581 acaaaattat gagattttca tcacacattt tgacatcagg aacggtatgc tgaattcacc
     5641 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
     5701 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
     5761 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
     5821 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
     5881 accgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
     5941 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
     6001 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
     6061 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
     6121 cataccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgtcgtg
     6181 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct tttttattga
     6241 taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc
     6301 agcgcgctgg atgaaaaatt attagcgtta ctggcagaac ggcgcgaact ggccgtcgag
     6361 gtgggaaaag ccaaactgct ctcgcatcgc ccggtacgtg atattgatcg tgaacgcgat
     6421 ttgctggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
     6481 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccaacaa
     6541 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggttct
     6601 tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt cattgaaagt
     6661 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     6721 gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga tctgctgcaa
     6781 cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttggtc
     6841 tccggcacta ctgatttatc caccatcaat acggtctaca gccatccgca gccattccag
     6901 caatgcagca aattccttaa tcgttatccg cactggaaga ttgaatatac cgaaagtacg
     6961 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc gttgggaagc
     7021 gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc aaatcagcga
     7081 caaaacttca cccgatttgt ggtgttggcg cgtaaagcca ttaacgtgtc tgatcaggtt
     7141 ccggcgaaaa ccacgttgtt aatggcgacc gggcaacaag ccggtgcgct ggttgaagcg
     7201 ttgctggtac tgcgcaacca caatctgatt atgacccgtc tggaatcacg cccgattcac
     7261 ggtaatccat gggaagagat gttctatctg gatattcagg ccaatcttga atcagcggaa
     7321 atgcaaaaag cattgaaaga gttaggggaa atcacccgtt caatgaaggt attgggctgt
     7381 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgt
     7441 gaatcatccg gcactggatt attactggcg attgtcattc gcctgacgca ataacacgcg
     7501 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     7561 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     7621 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataatgatgt cggcataaag
     7681 ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga
     7741 agagagcgcc agaagttgct caagctgaac attttcttct gccaggtgca gcccgtaagc
     7801 aaaagtagca aagtggcgca gtgcctgaat aaacgccata ttctgatcgt gctcgacggc
     7861 gctaatacga tgcagccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     7921 ttccggttta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg
     7981 accgaacatc gggtgtagcc ccagcaccgg accatcatgc gccgccagca tggcctgtaa
     8041 tggcccattt ttcactgatg ccagatcgac cagaatacaa tctttcggta aaggcggtaa
     8101 tttgccaata acttgctcag taacgtggat tggcacacta acaatcacca ttccggcatc
     8161 ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     8221 cgagagggtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac
     8281 caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac tggagtaaga
     8341 ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     8401 cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     8461 tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct tcgctaataa
     8521 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
     8581 cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc tgatgaattt
     8641 cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt
     8701 atttcatttc actgcgcggt tgctcggaag actgattgcc ctcgtggata ttactttcga
     8761 tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca
     8821 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
     8881 acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
     8941 agttcggcgc tttaccaccg cgcaggatca catggccgtc cggattcccc tgagtttgta
     9001 gcaacgcaac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcgc
     9061 gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa ccaaccggca
     9121 tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg
     9181 ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg
     9241 ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg
     9301 cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag
     9361 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
     9421 cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
     9481 aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct gaaatgcttt
     9541 tacgcgagtc agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg gccttcagtt
     9601 gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtcttttt
     9661 gcatgatggc gatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt
     9721 aacgataaca cataaagtaa agttttcaat ccatatttcg tacattttta tttacacagg
     9781 caatttagtc gcgctttcaa cccttacctc tgtatagata aatttacact ccctttgaaa
     9841 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg
     9901 ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc
     9961 agcaccgact ggtatgatgt cggtatggaa gatgcgatat cgggcagcgc cataaaagat
    10021 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
    10081 ggacaaaaga aaacttgcca gaccgatttt acttatgccc gaggactttc cggtaaaagc
    10141 tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt atggcaaaaa
    10201 ggggctgatg aaaatg
//
LOCUS       DAJOSY010000136        10146 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJOSY010000136 DAJOSY010000000
VERSION     DAJOSY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.136"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(2..481)
                     /gene="folK"
                     /locus_tag="OHN27_003832"
     CDS             complement(2..481)
                     /gene="folK"
                     /locus_tag="OHN27_003832"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCQ2288197.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGEMLQNRLTILNTSQPVIW"
     gene            complement(478..1896)
                     /gene="pcnB"
                     /locus_tag="OHN27_003833"
     CDS             complement(478..1896)
                     /gene="pcnB"
                     /locus_tag="OHN27_003833"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCQ2288198.1"
                     /translation="MTLPRCTIFTRVANFCRKVLNREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNISDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1935..2861)
                     /gene="gluQRS"
                     /locus_tag="OHN27_003834"
     CDS             complement(1935..2861)
                     /gene="gluQRS"
                     /locus_tag="OHN27_003834"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCQ2288199.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKSDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2898..3353)
                     /gene="dksA"
                     /locus_tag="OHN27_003835"
     CDS             complement(2898..3353)
                     /gene="dksA"
                     /locus_tag="OHN27_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCQ2288200.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3531..4235)
                     /gene="sfsA"
                     /locus_tag="OHN27_003836"
     CDS             complement(3531..4235)
                     /gene="sfsA"
                     /locus_tag="OHN27_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCQ2288201.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAECSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4250..4780)
                     /gene="thpR"
                     /locus_tag="OHN27_003837"
     CDS             complement(4250..4780)
                     /gene="thpR"
                     /locus_tag="OHN27_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCQ2288202.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRAKHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4854..7283
                     /gene="hrpB"
                     /locus_tag="OHN27_003838"
     CDS             4854..7283
                     /gene="hrpB"
                     /locus_tag="OHN27_003838"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCQ2288203.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQILLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRTGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDNESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7479..10013
                     /gene="mrcB"
                     /locus_tag="OHN27_003839"
     CDS             7479..10013
                     /gene="mrcB"
                     /locus_tag="OHN27_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCQ2288204.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLDVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSSNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
BASE COUNT         2338 a         2625 c         2877 g         2306 t
ORIGIN      
        1 attaccagat tacaggttgt gaagtattca ggatagtaag tctattttgc aacatctccc
       61 catcaggaaa caccaactcc ggcgcgattt caaacagcgg ccacagcata aatccacgat
      121 tcttcatatc gtagtgcgga acggtcaggc gttcagtatt tatcacttca ttaccaaaca
      181 gcatgatgtc gagatccagc gtgcgtggtc cccagcgttc agctttgcgg acgcgacctt
      241 gctgcaattc aatacgctgt gtgtgattga gtagctcttc aggtgcaaga gaggtttcca
      301 gcgccacggc tgcgtttaag taatcgggtt gatcttgcgg ccccagcggt ggggtgcggt
      361 aaaacgaaga aacggtaaga atgtggcttt cagggatatc gcctaatgct ttcagggcag
      421 cattgacctg ctccagcgga gaggccagat tgctgcctat ggcaatatac gccactgtca
      481 tgcggtaccc tcacgacgtg gtgcgcgttt gcgtggacga cgagtacgac gacgcggtga
      541 cggttcttca tccagctcgt tgagcatccc tttttggtct ggtggcgcgg aaacctggaa
      601 ctcaccccac catttcacca gacgctgcag ttcagcgtta cgctcaactt cagctcgcaa
      661 ggccaacagg tcataagccg cacggaactt aggatgctcc agcagtttcc atgcgcgttt
      721 accctgacga cgggacatac gcaactgcaa ctgccagata tcgcgggtta atgtcgtcag
      781 acgtttcggg attgccagtg aacggcaggc ttcgtccagc acgtcgttca tcgccagcgc
      841 gaaagcgtcg tgataggtca ggccgctttc ctgggcgatc ttctgtgccg tctccagcag
      901 tgggtaccag aacatggcgg caaacaggaa cgccgggttc acgcgcatat cgttatggat
      961 acgcgtatcg gtattcttca gcacctgttc aatgatccgc tccatcgggc tgtcgccatt
     1021 ttccgtgaag tagcgggtaa tggtcgggaa cagcggctgg aacagatgat attcacacaa
     1081 cagcttatag gtttcgtaac cgtagcccgc ttgtagcagt ttaagcgatt cttcaaacag
     1141 gcgtgccggt gggatatcgt tcagcagggt agcgaggcga gggatcggtt ctgcggtttc
     1201 cgggctgatg cgcataccca atttggcggc aaaacgtacc gcgcgcagca tacgtaccgg
     1261 atcttcacgg tagcgcgttt ccgggttacc aatcagacgg ataacgccgt ccttcagatc
     1321 cttcatgccg ccaacgtaat cacggacggt aaaatccgct acgctgtaat acaggctgtt
     1381 gatagtgaaa tcgcggcgct gggcgtcttc ttcgatggag ccgaaaatgt tgtcgcgcag
     1441 caacatgccg ttttgcccgc gttgggaggt cgtgcggtcg ctgatgttac cttcgtggtg
     1501 tccacggaag gtcgcaactt cgataatctc cgggccaaac atcacatgag ccagacggaa
     1561 acggcgaccc acaaggcggc agttacggaa cagtttgcgc acctgctcag gcgtggcgtt
     1621 agtggttacg tcaaagtctt tcggcttttt gccaagtaac aggtcgcgca cgccgccgcc
     1681 aaccagccag gcttcgtatc ccgctttatt gagcctgtac attaccttca gggcattttc
     1741 actgatatct ttgcgggaaa tagcatgctg ctcacgcggg atcaccgtca cctgtggacg
     1801 ggcgactgcc tgttcagcct cgctttcctc gcggtttagc accttgcggc aaaaattagc
     1861 gactcgggta aaaatagtac acctcggtag tgtcaaacat cattcaggac aaaaaaatag
     1921 cggctaatca tagctcagca tgacgcattt gagaatgttg aatttacaat tgctgactca
     1981 ggcacggcgg taagccgcca gtttttgacg gctgactgga ggatttgctc gacgctgaaa
     2041 tcctgccagt gtgcttctgc ctgctgaccc agaaattgaa gtgccgcgat tagtaccggg
     2101 cgtggatcgc ttttcggcaa cgcaggcgca tgattctgct tggaaagttt agcgccttgt
     2161 ggattaagcg ccagcggcag atgaatgtaa tctggcactt tccagccaaa aagctggtac
     2221 agcgagattt gccgcactgt cggttcgatc aaatctgccc cgcggacaat ttcgctcacg
     2281 ccctggaagt gatcatccac caccaccgcc aggttatagg caaacaaacc atcacggcga
     2341 tgaataataa aatcttcccg tgccagtttt tcgtcggcgt gaataatgcc gcgcagctgg
     2401 tcagtaaatt gcgtgaccgg atgctgctgg cggatacgca ctgcggcgtt gtctggtcca
     2461 tgatgcaaca cccggcaatg accgtcgtaa ataccgccaa tgctttgaat acgcgcacgc
     2521 gtgcaggtgc agtaataact tagcccttgc tcatgtaacc aggcgagtgc ttcacgatag
     2581 gcgtcgtgac gttgcgattg ccagagaaca tcgccgtccc agtgcagacc gtaatgttcc
     2641 agctggcgca ggatagtttc tgcggcaccg ggaacttcac gaggcgggtc gatatcttct
     2701 atacgtacca gccagcgacc ttgccgggcg cgagcctgca aatagctgcc aagcgcagca
     2761 atcagagaac caaaatgaag ctcgccggaa ggagaggggg cgaagcggcc aatatagtgt
     2821 gtgtctgtca tctctttgaa caaaaaataa ggcgggagca tttcccgcct gtggtaaacg
     2881 tgatggaacg gctgtaatta gccagccatc tgtttttcgc gaatttcagc cagcgttttg
     2941 cagtcgatgc acagatcggc tgtcgggcgc gcttccagac ggcgaatacc aatttcaaca
     3001 ccgcaggatt cgcagtagcc gaaatcttcg tcttccactt ttttcagcgt cttctcgatc
     3061 tttttgatca gcttacgctc gcgatcgcgg ttacgcagtt cgaggctgaa ctcttcttcc
     3121 tgggctgcac ggtctaccgg gtccgggaag ttggctgctt catcctgcat atgtgtaacg
     3181 gtgcgatcga cttcatccct gagttgatta cgccatgctt ccagaatacg acggaagtgc
     3241 gccagctggg cttcattcat atactcttcg cccggcttct cctgatatgg ttccacccca
     3301 gcgatggcga gaatactcag ggacgatgtt ttacggtttt gcccttcttg catgttgctt
     3361 ctccttaaca cgcactatcg atccccatgt tcgggggaaa aatgaggccg ctataaatag
     3421 cagatgcttt tccggatagc aattatctaa acgtaacact tgacaactgt gtgaggaaaa
     3481 gcgtatttgc gcacgcgacc agaatgtaaa ttaaccagtt acttacttta ctacaatgta
     3541 accggcagtg attttttaag agccatgcct tcagcagaaa tttccgcttt gtaagccaga
     3601 atttctaccc ccctctgttg agcttctgac aatagttgcg cgtatttctc atcgatgtgg
     3661 cgcgcgggtg aaaaccgtgt aatggctgaa tgcagcacgg cgaaaaagat aaccgcacgc
     3721 tggccttcag ccgctacgct catcaactcc cgaaggtgtt tctgacctcg ttcagtgacc
     3781 gcatcgggaa aatatccctg ttcgttctcc gctaacgtaa ccgatttcac ttcaatatag
     3841 cagtctggac gcgaatccgc ctgcaacata aagtcaatac ggctgcattc tgcgccgtat
     3901 tttacttcgc ttttcagcga gctatagcct gacagttctg aaattgattc attaaggata
     3961 gcctctttcg tcaacctgtt agcccaaagc gtgttgacgc aaataaatgc gccgctctgg
     4021 ctttgagtta attcccaggt gtgtgggtat ttccgtttgg tgttgtctga agtcgaatac
     4081 cagacggtat cgccaggcgt tgcacaaccg gtcatcgcac ccgtattcgg gcagtgtagc
     4141 gttaattcgc gaccatcggg tgtgatcaca tcggctaaaa aacgtttgta acgctgaatt
     4201 agcgtcgcgc gctgtagagg gggagaaaat tccattgcga caatccttgt tattgcgtta
     4261 gcgcccagcg ttttagcggc gtgtagcgtg tgcgtccacg ggcaaacgag gaggcgtaaa
     4321 gggtgaactc cgtcaccgca taagaccagt taaaacccgg cggcgggatt gtcaccgcct
     4381 cgctggcgtc gcgcaataag gtaatatgtg gatgaaacgg acgattgctt tgaaaacaac
     4441 cgctgcgggc agcctgtgaa cggagcatat tcgccagctg gattaagccg cgtggaggct
     4501 gacgcatccc taaccacacc acacgcgaac gcagccattg tccggcgtca tcaagcgtga
     4561 gtgtgaaacc aggttgacga atccgtccgg ctaaaagaga aagcgccttc tctttctcgg
     4621 cgctcacttc gcctaaaaat gccagagtca gatgcaaatt atcggcggcg accggacgtc
     4681 ccgcctcagg tgggaagtgt ttggcgcgcc agtggataat ctgttcgcgg atttctgcag
     4741 gtaagtcgat agcaaagaac agacgttgcg gttcagacat gtgaggcact cggttatgaa
     4801 ttaccgcgat gctacaatgt gccgcgaaga atgttaaccc tctggagcgt tttgtgtcgt
     4861 cgttgcccgt tgctgccgtc ttacctgaat tacttaccgc tctcgattgt gcgccgcaga
     4921 tattattaag tgcgccgacc ggagccggga aatcaacctg gctgccgctg caactgctgg
     4981 cgcatcccgg cattaacggg aaaattatcc tcctggagcc gcgtcgtctg gcggcgcgta
     5041 acgtcgcgca acggctggcg gagctgctta acgaaaagcc aggcgatacc gttggctacc
     5101 ggatgcgtgc gcaaaactgc gtcgggccaa atacccgcct ggaagtggtt accgaaggcg
     5161 tgctgacgcg catgatccag cgtgacccgg aactgagcgg tgttggattg gtgatccttg
     5221 atgaatttca tgagcgcagc ttgcaggcgg atttggcgtt ggcgctgtta ctcgatgtgc
     5281 aacaaggtct gcgtgatgac cttaaactgc tgattatgtc ggctacgctg gacaacgacc
     5341 gcttgcagca aatgctgcca gaagcgcccg tcgtcatctc agaagggcgc tcgtttccgg
     5401 ttgaacgcca ttatttaccg ctgcccgcgc atcagcgttt tgacgaagcc gttgcggtag
     5461 ccaccgccga aatgctgcgt caggaaagcg gatcattact gttattttta cctggcgtcg
     5521 gagaaattca gcgtgtgcag gaacaactgg cttcgcgcat cggcagtgat gtgttgctct
     5581 gcccgctgta tggcgcgttg tcgctgaacg atcagcgaaa agcgatcctc ccggcaccgc
     5641 aagggatgcg caaagtggtg ctggcgacca atattgctga aaccagttta accatcgaag
     5701 gtattcgtct ggtggtggat tgtgcccagg agcgtgtggc gcgttttgag ccgcgcacgg
     5761 ggcttacgcg actgattact caacgcgtta gccaggcatc catgacgcag cgtaccgggc
     5821 gcgccgggcg tctggagccg ggtatctgcc tgcatttaat cgccaaagaa caagcagaac
     5881 gcgctgccgc gcaaagtgaa ccggagatct tacaaagcga tctttccggt ttgctgatgg
     5941 aattactgca atggggatgc agcgatccgg cgcagatgag ctggctggat caaccgccag
     6001 tagtgaatct aatggccgcg aaacgtctgt tacaaatgct gggggcgctg gacggtgaac
     6061 ggcttagtgc gcaagggcaa aaaatggcag cgctgggtaa cgatccgcgt ttagcggcaa
     6121 tgctggttag cgcgaagaac gacgacgaag ctgctaccgc ggcaaaaatt gccgccattc
     6181 tcgaagagcc gccacggatg ggcaatagtg acctgggcgt ggcgttttcg cgcaatcaac
     6241 cagcctggca gcaacgtagt cagcaactgt taaaacgctt aaacgtacgc ggcggtgagg
     6301 cagacagttc gcttatcgcg ccgctacttg ccggggcgtt tgccgatcgc attgctcatc
     6361 gccgtgggca agatggacgc tatcaactgg caaacggcat gggagcgatg ctcgatgccg
     6421 acgacgcgct aagccgccac gaatggttga tcgcaccgtt attattgcag ggcagcgcct
     6481 cgccggatgc gcggatttta ctggcgctac cggtcgatat tgatgaatta gtacaacgct
     6541 gtccgcagct ggtgcagcaa tctgacaccg tggaatggga tgacgcgcaa ggtacgctga
     6601 aagcctggcg tcggctgcaa atcggtcagt tgacggtgaa agtgcagccg ttagcgaaac
     6661 cgtcagaaga cgagttgcat caggcgatgc ttaatggcat ccgtgataaa ggtttaagcg
     6721 tgctcaactg gacggcggaa gcggaacagc tacgcttgcg tttgttatgc gccgcaaagt
     6781 ggttgccgga atatgactgg cctgcggttg ataatgaaag tttgttggcg acgctggaaa
     6841 cgtggctgct gccacatatg accggcgtgc attcactacg cggcctgaaa tcactcgaca
     6901 tttatcaggc actacgcgga ttacttgatt ggggaatgca gcaacgtctg gatagtgaat
     6961 tgcctgcgca ttacactgtg ccgacgggaa gccggatcgc cattcgttat catgaagata
     7021 acccgcccgc gctggcggtg agaatgcagg agatgtttgg tgaggccacc aatccgacga
     7081 tcgcccaggg gcgcgtgccg ctggtgctgg agttgctttc ccctgcccaa aggccattac
     7141 aaatcacacg agatttgagc gccttctgga aaggagcgta ccgtgaggtg caaaaagaga
     7201 tgaaagggcg ttatcccaaa catgtctggc cggacgaccc ggcaaacact gcaccgacgc
     7261 gacggacgaa aaagtattcg taaggttggt tttctccctc tccctgtggg agagggccgg
     7321 ggtgagggca tcagcgcgca cgttcaccct caccctaacc ctctccctca agggagaggg
     7381 gaccgatcga gcacaaattt tgagagatat cttcttctgt cttgtaacag aagaacagaa
     7441 aatcgggctt ttgcgcctga atattgcgga gaaaaagcat ggccgggaat gaccgcgagc
     7501 caattggacg caaagggaaa ccgacgcgtc cggtcaaaca aaaggtaagc cgtcgtcgtt
     7561 acgaagatga cgatgattac gacgattatg atgactatga ggatgaagaa ccgatgccgc
     7621 gcaaaggtaa gggcaaaggc aaagggcgta agcctcgtgg caaacgcggc tggctatggc
     7681 tactgctaaa actggctatc gtttttgccg tgctgatcgc catttacggc gtttatctcg
     7741 atcaaaaaat tcgtagccgt attgatggca aggtctggca actgcctgcg gcagtttatg
     7801 gccgaatggt caatcttgag ccagacatga ccatcagcaa gaacgagatg gtgaagctgc
     7861 tggaggcgac ccagtatcgt caggtgtcga aaatgacccg tcctggcgaa tttaccgtgc
     7921 aggctaacag cattgagatg atccgccgtc cgtttgattt cccggacagc aaagaagggc
     7981 aggtgcgtgc gcgtttgacc tttgatggcg atcatctggc gaccatcgtc aatatggaga
     8041 acaaccgtca gttcggcttc ttccgtcttg atccgcgcct gatcaccatg atctcctcgc
     8101 caaacggtga acaacgtctg tttgtgccgc gcagtggttt ccctgatttg ctggtggata
     8161 ctttgctggc gacagaagac cgtcattttt acgagcatga cggcatcagc ctctactcca
     8221 tcggacgtgc ggtgctggca aacctgaccg caggtcgcac ggtgcagggt gcgagtacgc
     8281 tgacgcaaca gctggtgaaa aacctgttcc tctccagcga gcgctcttac tggcgtaaag
     8341 cgaacgaagc ctatatggcg ttgatcatgg atgcgcgtta cagcaaagat cgcattcttg
     8401 agctgtatat gaatgaggtg tatctcggtc agagcggcga caacgaaatt cgtggcttcc
     8461 cgctggcgag cctgtactac tttggtcgcc cggtagaaga attgagcctt gaccagcagg
     8521 cgctgttagt tggcatggtg aaaggggcgt ccatctacaa cccgtggcgt aacccgaaac
     8581 tggcgctgga gcgacgtaat ctggtgctgc gtttgctaca acaacagcag attatcgatg
     8641 gtgaactgta tgaaatgctc agcgcgcgtc ctctggacgt acagccgcgc ggtggggtaa
     8701 tctctccaca gccagccttt atgcaactgg tgcgtcagga gttgcaggcg aaattaggcg
     8761 ataaggtaaa agatctgtcc ggcgtgaaga tcttcactac ctttgactcg gtggcccagg
     8821 acgcggcaga aaaagccgcc gtggaaggca ttccggcact gaagaaacag cgtaagctga
     8881 gcgatctgga aaccgcgatt gtggtcgtcg accgctttag tggtgaagtt cgtgcgatgg
     8941 tcggaggttc tgagccgcag tttgcgggct acaaccgtgc gatgcaggcg cgtcgttcga
     9001 ttggttccct tgcaaaacca gcgacttatc tgactgcctt aagccagccg aaaatctatc
     9061 gcctgaacac gtggattgcg gatgcgccaa tcgcgctgcg tcagccgaat ggccaggtct
     9121 ggtcaccgca gaacgatgat cgtcgttata gcgaaagtgg cagagtgatg ctggtggatg
     9181 cgttgacccg ttcgatgaac gtgccgacgg taaatctggg gatggcactg gggctgcctg
     9241 cggttacgga gacctggatc aaactgggcg tgccgaaaga tcagctgcat ccggttccgg
     9301 cgatgctgct gggggcgttg aacttaacgc caatcgaagt ggcgcaggca ttccagacca
     9361 tcgccagcgg cggtaaccgt gcaccgcttt ctgcgctgcg ttcggtaatc gcggaagatg
     9421 gcaaagtgct gtatcagagc ttcccgcagg cggaacgcgc tgttccggcg caggcggcgt
     9481 atctgacact atggaccatg cagcaggtgg tacaacgcgg tacgggtcgt cagcttgggg
     9541 cgaaataccc gaacctgcat ctggcaggga aaacagggac tactaacaat aacgtagata
     9601 cctggtttgc gggcattgac ggcagcacgg tgaccatcac ctgggtaggc cgtgataaca
     9661 accagccgac caaactgtat ggagccagcg gggcaatgtc gatttatcag cgttatctgg
     9721 ctaaccagac gccaacaccg ctgaatcttg ttccgccaga agatattgca gatatgggcg
     9781 tggactacga cggcaacttt gtttgcagcg gtggcatgcg tgtcttgccg gtctggacca
     9841 gcgatccgca atcgctgtgc cagcagagtg agatgcagca gcagccgtca agcaatccgt
     9901 ttgatcagtc ttctcagccg cagcaacagc cgcaacagca acctgctcag caagagcaga
     9961 aagacagcga cggtgtagcc ggttggatta aagatatgtt tggtagtaat taacgtctaa
    10021 gcgtgaaata ccggatggcg agttgccatc cggtaaaata acatcccacc caaagtatta
    10081 accctttctt ttcatctggt tgtttattaa cccttcagga acgctcagat tgcgtaccgc
    10141 ttgcga
//
LOCUS       DAJOSY010000137        10065 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJOSY010000137 DAJOSY010000000
VERSION     DAJOSY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.137"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            122..1459
                     /gene="adiC"
                     /locus_tag="OHN27_003840"
     CDS             122..1459
                     /gene="adiC"
                     /locus_tag="OHN27_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCQ2288205.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            1563..3206
                     /gene="eptA"
                     /locus_tag="OHN27_003841"
     CDS             1563..3206
                     /gene="eptA"
                     /locus_tag="OHN27_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCQ2288206.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            3203..3871
                     /gene="basR"
                     /locus_tag="OHN27_003842"
     CDS             3203..3871
                     /gene="basR"
                     /locus_tag="OHN27_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCQ2288207.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            3872..4972
                     /gene="pmrB"
                     /locus_tag="OHN27_003843"
     CDS             3872..4972
                     /gene="pmrB"
                     /locus_tag="OHN27_003843"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCQ2288208.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDDGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(4948..5037)
                     /gene="pmrR"
                     /locus_tag="OHN27_003844"
     CDS             complement(4948..5037)
                     /gene="pmrR"
                     /locus_tag="OHN27_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCQ2288209.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5149..6651)
                     /gene="proP"
                     /locus_tag="OHN27_003845"
     CDS             complement(5149..6651)
                     /gene="proP"
                     /locus_tag="OHN27_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCQ2288210.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(6915..7793)
                     /locus_tag="OHN27_003846"
     CDS             complement(6915..7793)
                     /locus_tag="OHN27_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCQ2288211.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(7790..10018)
                     /gene="crfC"
                     /locus_tag="OHN27_003847"
     CDS             complement(7790..10018)
                     /gene="crfC"
                     /locus_tag="OHN27_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCQ2288212.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQPLFTAE
                     RY"
BASE COUNT         2485 a         2667 c         2520 g         2393 t
ORIGIN      
        1 aagagctata ttaataacgg catcagcgat aacccggtcg ataataattc aactatcgaa
       61 tgcaggcgta tgatatgacg taatttattg tcacgaagct cgccttcgca ggagtttaat
      121 tatgtcttcg gatgctgatg ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc
      181 ggggaatatt atggggtcag gtgtttttct gttacctgca aacctggcct ctactggcgg
      241 gattgccatt tatggatggt tggtgacgat tatcggtgca ctggggctct cgatggtata
      301 cgccaaaatg tcgttcctcg acccaagtcc tggtggttct tacgcttacg cccgccgctg
      361 ctttggcccg tttctcggtt atcaaaccaa cgtcctctac tggctggcct gctggatcgg
      421 caatatcgcc atggtggtca ttggcgtagg atatttaagt tacttcttcc cgattctgaa
      481 agatccattg gtattaacca tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa
      541 cattgtcggt ccgaaaatga tcacccgtgt gcaggcagtt gccaccgtgc tggcgctgat
      601 ccccatcgtc gggattgccg tatttggctg gttctggttc cgtggtgaaa cctatatggc
      661 ggcatggaac gtcagcggcc tgggcacctt cggtgcaatt caaagtaccc ttaacgttac
      721 gctgtggtcg ttcatcggtg tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc
      781 gaaacgcaat gtccctatcg ccaccattgg tggggtattg attgccgccg tttgctatgt
      841 actttctacc accgcgatta tggggatgat ccctaatgcc gcactgcgcg tttctgcttc
      901 gccattcggt gatgccgcac ggatggcgtt gggtgacacc gccggggcca ttgtttcctt
      961 ctgcgcagct gcgggttgct taggttcact gggcggctgg acgttgctgg cgggtcaaac
     1021 ggcgaaagcc gctgccgatg acggactgtt cccaccgatt tttgcccgtg taaataaagc
     1081 gggtacgcca gtggcggggc tgattatcgt cggtattttg atgaccatct tccagctcag
     1141 cagcatttca ccaaacgcga ccaaagagtt cggtctggtt tcttccgtct cggtcatctt
     1201 tacactggtg ccatatcttt acacctgtgc ggcgttactg ctgctcggac acggtcactt
     1261 tggtaaagca cgcccggcat atctggcagt tactaccatt gccttcctct actgcatctg
     1321 ggccgtggtg gggtccggag cgaaagaggt tatgtggtca tttgtcaccc tgatggtcat
     1381 caccgccatg tatgcgctga attacaaccg gctacataaa aacccgtatc ccttagatgc
     1441 accaataagc aaagattaat tccccttaat ccagcaaaca taaaagccaa ccttaagaac
     1501 ttaaggttgg cttaattttg ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg
     1561 tgatgttgaa gcgcctacta aaaagaccct ctttgaattt actcgcctgg ctattgttgg
     1621 ccgcttttta tatctctatc tgcctgaata ttgccttttt taaacaggtg ttgcaggcgc
     1681 tgccgctgga ttcgctgcat aacgtactgg ttttcttgtc gatgccggtc gtcgctttca
     1741 gcgtgattaa tattgtcctg acactaagct ctttcttatg gcttaatcga ccactggcct
     1801 gcctgtttat tctggttggc gcggctgcac aatatttcat aatgacttac ggcatcgtca
     1861 tcgaccgctc gatgattgcc aatattattg ataccactcc ggcagaaagt tatgcgctga
     1921 tgacaccgca aatgttatta acgctgggat tcagcggcgt gcttgctgcg ctgattgcct
     1981 gctggataaa aatcaaacct gccacctcgc gtctgcgcag tgttcttttc cgtggagcca
     2041 atattctggt ttctgtacta ctgattttgc tggtcgccgc actgttttat aaagactacg
     2101 cctcgttgtt ccgcaataac aaagagctgg tgaaatcctt aagcccctct aacagcattg
     2161 ttgccagctg gtcatggtac tcccatcagc gactggcaaa tctgccgctg gtgcgaattg
     2221 gtgaagacgc gcaccgcaac ccgttaatgc agaacgaaaa acgtaaaaat ttgaccatcc
     2281 tgattgtcgg cgaaacctcg cgggcggaga acttctccct caacggctac ccgcgtgaaa
     2341 ctaacccgcg gctggcgaaa gataacgtgg tctatttccc taataccgca tcttgcggca
     2401 cggcaacggc agtttcagta ccgtgcatgt tctcggatat gccgcgtgag cactacaaag
     2461 aagagctggc acagcaccag gaaggcgtgc tggatatcat tcagcgagcg ggcatcaacg
     2521 tgctgtggaa tgacaacgat ggcggctgta aaggtgcctg cgaccgcgtg cctcaccaga
     2581 acgtcaccgc gctgaatcta cctgatcagt gcatcaacgg cgaatgctat gacgaagtgc
     2641 tgttccacgg gcttgaagag tacatcaata acctgcaagg tgatggcgtg attgtcttac
     2701 acaccatcgg cagccacggt ccgacctatt acaaccgcta tccgcctcag ttcaggaaat
     2761 ttaccccaac ctgcgacacc aatgagatcc agacctgtac caaagagcaa ctggtgaaca
     2821 cttacgacaa cacgctggtt tacgtcgact atattgttga taaagcgatt aatctgctta
     2881 aagaacatca ggataaattt accaccagcc tggtttatct ttctgaccac ggtgaatcgt
     2941 taggtgaaaa tggcatctat ctgcacggtc tgccttatgc catcgccccg gatagccaaa
     3001 aacaggtgcc aatgctgctg tggctgtcgg aggattatca aaaacggtat caggttgacc
     3061 agaactgcct gcaaaaacag gcgcaaacgc aacactattc acaagacaat ttattctcca
     3121 cgctattggg attaactggc gttgagacga agtattacca ggctgcggat gatattctgc
     3181 aaacttgcag gagagtgagt aaatgaaaat tctgattgtt gaagacgata cgctgttatt
     3241 gcagggactg attctggcgg cgcaaaccga aggctacgcg tgcgatggcg tgacaaccgc
     3301 gcggatggcg gaacaaagcc ttgaggccgg tcattacagc ctggtggtac tggatttagg
     3361 gttacccgac gaagatggac tgcattttct cgcccgtatc cggcagaaaa aatacaccct
     3421 gccggtactg atcctcaccg ctcgcgatac gctgaccgac aaaatcgccg ggctggatgt
     3481 cggtgccgat gactatctgg tgaagccctt tgcgctggaa gagttacacg cccgtatccg
     3541 agccctgcta cgacgccata ataatcaggg cgaaagtgag ctgattgttg gcaatctgac
     3601 gctgaatatg ggtcgccgtc aggtatggat gggcggtgaa gagttgattc tgacgcccaa
     3661 agaatatgct ctgctgtcac ggttaatgct caaagcaggc agtccggtgc atcgggaaat
     3721 tctctacaac gacatctata actgggacaa tgaaccctcg accaacaccc tggaagtgca
     3781 tatccacaat ctgcgcgaca aagtgggcaa agcccgtatc cgcaccgtgc gcggctttgg
     3841 ctatatgctg gtcgcgaatg aggaaaacta attgaatctg atgcgttttc tgcgccgacc
     3901 aatatcgctg cgccaacggc tgatattgac catcggggcc attttgttgg tgtttgagct
     3961 gatcagcgtc ttctggctat ggcatgaaag taccgagcag attcagctgt ttgagcaggc
     4021 gctacgcgac aatcgcaaca acgatcgcca catcatgcgt gagatccgcg aggcggtcgc
     4081 cagcctgatt gtccccggcg tctttatggt cagcctgacg ctatttatct gctatcaggc
     4141 ggtacgccgc atcacccgcc cgctggcgga gctgcaaaaa gagctggaag cgcgcaccgc
     4201 cgacaactta acgcccattg ccattcacag cgccaccctc gaaatcgaag cggtggtttc
     4261 ggcgttaaac gatctggtca gtcgcctgac cagcacgctg gataacgaaa ggttgtttac
     4321 cgctgacgtc gcgcacgaac tgcgaacgcc actggcgggg gtgcgtttgc atctggaact
     4381 gctggcgaaa acgcatcaca ttgatgtagc accgttagtg gcacggcttg atcagatgat
     4441 ggagagcgtc tcccagctgc tgcaactggc gcgtgccgga cagtcatttt cttccggtaa
     4501 ttatcaacat gtaaaactgc tggaagatgt cattctcccc tcgtatgacg aactcagtac
     4561 catgctcgac cagcgacagc aaaccctgct attgccagag agcgccgccg acatcaccgt
     4621 tcagggtgat gcaaccctgc tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg
     4681 ttacagcccg caaggcagca acattatgat taagctgcaa gaagatgacg gagcggtcat
     4741 ggcagttgaa gatgaaggac caggtattga tgagagtaaa tgcggggagt tgagtaaagc
     4801 gtttgtacgt atggacagcc gttatggcgg gattggtctg gggttaagta ttgtcagccg
     4861 cattacacag ttgcatcacg ggcagttttt cctgcaaaac cggcaagaga cttccggcac
     4921 gcgggcctgg gtacggctga agaaagatca gtacgtggca aaccagatat aaagaaagct
     4981 gctgaccacc agcacgctga acacggtagt taaactttca taaacacggt ttttcattgc
     5041 actctcctcc gttaacctgg aggagagtat gcgcgtcaga gattaagtga accttaagag
     5101 ttcaattggc ctgataagac agcgtcacat caggccatcc gtttcagctt attcatcaat
     5161 tcgcggatgt tgctgcacca ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc
     5221 aatattatcg attttctgct cgatattatc gtaatgctcg acgagaattt ccttcgcttc
     5281 ctgtatatct gacgccgccg gtgtcgcacc tttcaacgga cgatttgccg tctctttcat
     5341 ggttacgccg gtgattaaac caatcaccgc cactaccatc aggtaatagg caggcatcat
     5401 cagattctgc gagctttcga ccagccaggc cgccagtgtt ggcgtcagac cggcaaccag
     5461 caccgaaata ttaaatgccg ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc
     5521 tggcaaggta gaagccataa cgcccgtaaa gcagttaagg atcaccgcca gcatcagtaa
     5581 cccggcaaaa atcaggccga tgacgttact gttaatcaga ataaacgccg ggatcgccaa
     5641 cacaaacagg gcaacactac caagtagcac aaacggacga cggccaaaac ggtcactcag
     5701 caagcccatc accggctgga caaacagcat accgatcata atggcgataa taatcagcac
     5761 cccgtggtct tcggagtaat gcaagttatg cgacaaataa ctcggcatat aggtcagcaa
     5821 catgtagtaa gtcacgttgg tggcaattac cagaccaata catgtcaaca ggctgcgcca
     5881 gtatttagtg gcaatctctt taaacgagac tttcgggcca tcctgcaaac cttcgcggtc
     5941 gccctgttcc agtttatcaa catgctgctg gaacgccgga gtttcttcca gcgcatggcg
     6001 caggtaaagc ccgataatcc ctaacggcag agcaataaag aacggaatac gccagcccca
     6061 gtcgaggaag ttcgcttcgc cgacaatggt cgaaattaac accaccacgc ccgcacccag
     6121 cacaaacccg gcaatagaac cgaagtccag ccagctgccc ataaagccac gtttacggtc
     6181 aggggagtat tccgcaacaa atatcgacgc cccggtatat tcaccgccga ccgagaaacc
     6241 ttgtgccatc ttacagatca acagcagaat cggtgcccaa ataccaatcg tgtcgtagga
     6301 cggtattaag ccaatacaga acgtactgat cgacataatc acaatagtga tagcgaggat
     6361 cttctggcga ccatatttat cgcccaacat accaaagaag agtccgccaa gcggtcgaat
     6421 cagaaaggga acggagaaag tggcaagtgc agcaaccatc tgcacgctgg ggtcagcccc
     6481 cgggaaaaaa actttaccta atgcgtaagc aacaaaacca taaacaccaa aatcgaacca
     6541 ttccattgca ttacccagtg atgctgcggt aatggcttta cgcagtttac cgtcatcaat
     6601 aatggtgaca tcacgaaggg taatcggttt tacttttttc cttttcagca tagctttcct
     6661 cgcagagttg gcccatttcc gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg
     6721 agcgggaatt gcaggatgca atctctgtaa caagcgtagc gggtttgctt acaccctccg
     6781 gttaaggaat gtacaatcaa accagttgac gcctgaattg ggcagttaat gaccttttta
     6841 ccctaccagt gtttaaattt gtgatcaact tcacacatgg atttcgttta ggactcattg
     6901 atgtaactga ttatttacgc caaatttaca gagaagattt cctcgaccat tgcccccacc
     6961 aaacgctgcg ctgaacagag tcgtggcatc cccagcgcga cagtttgcca acgttgggtc
     7021 actgaccagc aaacgggttg tttatctgcc gtggtccact ttcccagcca acttaaaaaa
     7081 tcagcatgat ccccgcccgt cggaacggtc gaagaggcaa accactgatg gtaatagtga
     7141 cgcgtggtcg gggccgcatt gatactctct gccagatgtt gtgtcgcttt cacgcgcaaa
     7201 tcacccagca acaacgccac cgcttgcggg cttcccagac gacaggcatc gccaaatgcc
     7261 ccccaacgca actcttcgag aacgataaaa cagcgcccag gcaacgacca ggcgttatac
     7321 gccccagcac gccagcgggt aaaaatttgc tcgctctgtt cacccgcctg caccagtagg
     7381 cctcgttgcg ttaatgcctt ttccttccag cgacaacggg ctgaaaacca ctcatacaag
     7441 gcggcaattt gctgggggag ctccgggaaa gcagagtgct ggcggtgatc cattttttgc
     7501 agcagcgcca gatgctgcgc gatctgcgtc agtgccagat gaccgggatc cagcatcgac
     7561 gccagttttt ccaccaatgt cagacgcagg ctaagcggcg catttaacat gccgccgttc
     7621 gtccatgtct gaagctgcac gcgcgcaaga agttcctgca tcagtcgttc acgaaactgc
     7681 tgctgatggg cttgtggaag gcgggcatca tcgccctgcg ccagatccac taaaaagcgg
     7741 ggataaaccg actccagcac gcgaccgggg ccgtcaagta acgtcttggt caatatcgtt
     7801 ctgccgtgaa aagtggttga atatcatcgc gtaacagctg ggcgtcggtg taaatccagc
     7861 cgtgagtcat cacagtctgc tgcaattgct gctgcatcag cctgaccacc gattcatttt
     7921 gttgacgcag agccagactt tcgcgtaaac gcgtctgtaa ttccgtcaaa catgaagcga
     7981 actcagcgaa aaaagtattc atgcctgccg taacagattc atcgacctgc tctgccagaa
     8041 ctttagccat ttgttggcaa taaagatcga cttctgcgct taatgctcgt tgcaacacac
     8101 tgtaatcaac cgtttctgtc ggggatttct catttccccg tccccagtca ggctgattca
     8161 accagcgcga aaaagtctca cgcacaacgc ctaatcgcgt gctctgctcg tccgttgctt
     8221 cctggcgcga aatgacttca ctgaacagct ggcgagtgtt gaagtgggga actacgccgt
     8281 gaaaaacagg aaaatgaaac ccaggacgaa accctgactc gctcaattcc attttgactt
     8341 gttgctcaat ggggcgaata acatcggtta acacccggca aagggtggat tcaagctcgg
     8401 caaaacgcag cgtaaagtcg cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac
     8461 agcgggtacg aatctcgctt aacggctccg aatcatcctg aaacaaggcg gctaactgcg
     8521 cattcagcgc atcttgttgt tgacgcagaa agtggttggc ggaggtcagg gccagctcga
     8581 tttcatgttt aatctcgccg ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt
     8641 cttcgacctg atggatattt tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac
     8701 gaaaatccag gtattcccgc gcctgctgcg cgtaattcaa cagtttatgc gcagcagatc
     8761 gcagagcata caacgaggcg ttagcgtaag cggcatgaag caacgcctga attggctggg
     8821 cgaacagcga atcttcccac aactgatcgg cagcatgacg aatatgttcg aggtccgcca
     8881 gatcggcatg acgccagcgc ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt
     8941 gttgctctgg cgcgggtaac ttaccgttgt tggctaactc atggcgcgcc cgattcgcca
     9001 ggtagcccca catcgacgac accggaaata tctgctgtgg cgtaatacag cctttcatca
     9061 gcgtcccgga aatcagtgcc cgcacctggt cggcgtcgtc actgttacga tcctgttgat
     9121 cgaacttatt gaccagcaca tacagcggca ccgattgccc caccgccaaa atcgcctcac
     9181 ggacctcttc atcggagatc gatttcagtt gcgtataatc cagcaccgcc agtaccgccg
     9241 aggcgcgtgc cagctgctgg ttaagcattt tttgcagatg cggttgcccg gcttcatttg
     9301 gcccgggggt atccagtagc gtcaactgac cgggataact ctccagcccc gccagatgga
     9361 caaactccac ttcaatcacg ggaatatgct caatggcggc gtaagcagaa aaaggaaaat
     9421 cgacgtccag cgccttcgcc agtcgcacta aatcattcaa acttttcaga caatgaaaaa
     9481 taggctgggc acccagataa tatttttcga aagcgacgcc attttcgatc cgctgcataa
     9541 gcgcacgcat atctttatct atttccagca catcggtcag atgcttaata tcgcaatcac
     9601 gcaggcgctg ttgtaattgt tgaattaaac ggtcgattgg cgcgacatgt gaaaaatgca
     9661 gtaccggttc tttttgcccg ggcgtatggc gaataagcgt cggcagcgca gtcattgggc
     9721 gattacgatt aggcagaacc tccgtaccaa caatggcatt aatggtggtt gatttccctg
     9781 ctttcatggt accgacaatt gcaagcacca tttccagtcg ggaaatttta cgcaactcat
     9841 tattcagcat cgcgtgacgt tcggcgatat taggctgact ccagggtaaa gccagttgtg
     9901 gcgcgtcgtc tccgggtaca gagagaggca ttttttccag taactgcaac tgttggcgag
     9961 aaagttgtaa caggcgttca gcctcctgac ttaactcata cagggtctgt gtgtacatag
    10021 aaaattcttc cttaaagcaa attttgttat tttatttagc cagat
//
LOCUS       DAJOSY010000138        10029 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJOSY010000138 DAJOSY010000000
VERSION     DAJOSY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.138"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            66..2243
                     /gene="iroN"
                     /locus_tag="OHN27_003848"
     CDS             66..2243
                     /gene="iroN"
                     /locus_tag="OHN27_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore salmochelin receptor IroN"
                     /protein_id="HCQ2288213.1"
                     /translation="MRINKILWSLTVLLVGLNSQVSVAKSSDDDNDETLVVEATAEQV
                     LKQQPGVSVITSEDIKKTPPVNDLSDIIRKMPGVNLTGNSASGTRGNNRQIDIRGMGP
                     ENTLILIDGVPVTSRNSVRYSWRGERDTRGDTNWVPPEQVERIEVIRGPAAARYGSGA
                     AGGVVNIITKRPTNDWHGSLSLYTNQPESSDEGATRRANFSLSGPLAGNALTTRLYGN
                     LNKTDADSWDINSPVGTKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIY
                     AGDTQNSSSSAVTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRM
                     NEGLSGGGEGRILVGEKFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDP
                     SSTSLTVNDSDISGISGSAADRSSKNHSQISALYIEDNIEPVPGTNIIPGLRFDYLSD
                     SGGNFSPSLNLSQELGDYFKVKAGVARTFKAPNLYQSSEGYLLYSKGNGCPKDITSGG
                     CYLIGNKDLDPEISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQTASGAY
                     ILKWQNGGKALVDGIEASMSFPLVKDRLNWNTNATWMITSEQKDTGNPLSVIPKYTIN
                     NSLNWTITQAFSASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDI
                     NKNLRLNVGVSNILNKQIFRSSEGANTYNEPGRAYYAGVTASF"
     gene            complement(2288..3244)
                     /gene="iroE"
                     /locus_tag="OHN27_003849"
     CDS             complement(2288..3244)
                     /gene="iroE"
                     /locus_tag="OHN27_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HCQ2288214.1"
                     /translation="MYAREYRSTRPHKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSVTSFDSVDGTRHYRVWTAVPNTTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAESRKTDLHSGRFSRKSGGSNNF
                     RQLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDSWLSSSYFRSYYSASPSLGRG
                     YDALLSRVTAVEPLQFFAKHLAIMEGSATQGDNRETHAVGVLSKIHTTLTILKDKGVN
                     AVFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(3329..4558)
                     /gene="iroD"
                     /locus_tag="OHN27_003850"
     CDS             complement(3329..4558)
                     /gene="iroD"
                     /locus_tag="OHN27_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroD"
                     /protein_id="HCQ2288215.1"
                     /translation="MLNMQQHPSAIASLRNQLAAGHIANLTDFWREAESLNVPLVTPV
                     EGAEDEREVTFLWRARHPLQGVYLRLNRVTDKEHVEKGMMSALPETDIWTLTLRLPAS
                     YCGSYSLLEIPPGTTAETIALSGGRFATLAGKADPLNKMPEINVRGNAKESVLTLDKA
                     PALSEWNGGFHTGQLLTSMRIIAGKSRQVRLYIPDVDISQPLGLVVLPDGETWFDHLG
                     VCAAIDAAINNGRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQ
                     RQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSRPGLFSET
                     DTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHD
                     YAWWRGALIDGIGLLQG"
     gene            complement(4662..8321)
                     /gene="iroC"
                     /locus_tag="OHN27_003851"
     CDS             complement(4662..8321)
                     /gene="iroC"
                     /locus_tag="OHN27_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin/enterobactin export ABC transporter
                     IroC"
                     /protein_id="HCQ2288216.1"
                     /translation="MPANHTPTPAQSWIVRLARVCWERKKLSVIVVVASVSTILLASL
                     TPLLTRQAVNDALAGNPARLPWLACGLLLIAFFDFIGNYVRRGYAGMLSLWVQHTLRG
                     RVFDSIQKLDGAGQDALRTGQVISRTNSDLQQVHTLLQMCPVPLAVFTYYIAGIAVML
                     WMSPAMTLIVVCVLVCLAITALRARRRVFAQTGLASDQLANLTEHIREVLAQISVVKS
                     CVAEMRETHWLDRQSRQIVRVRIGAVISQAMPGATMLALPVLGQIVLLCYGGWSVMHG
                     RIDLGTFVAFASFLAMLTGPTRVLASFLVIAQRTQASVERVFALIDTRSQMEDGTESI
                     NSQVVGLELENMSFDYHHGDRHILSNISFSLRAGETVAVVGASGSGKSTLLMLLARFY
                     DPCSGKIWLNTSEGRQNLRDIRLEALRRRVGIVFEDAFLFAGTVAENIAYGHPQATAD
                     DIRRAAAAAGASDFINALPKGFDSLLTERGTNLSGGQRQRIALARALITAPDVLILDD
                     TTSAVDAVTEAEINTALGRYADEGHMLLVIARRRSTLQLASRVVVLDKGRMVDTGTPA
                     ELEARCPAFRALMTGDSDFLATSHNSHNELWPAEPATQDDVTDTGDKGFVARMTRVPE
                     NAVQQALAGKGRKVTSLLKPVAWMFVIAALLIALDSAAGVGVLILLQHGIDSGVAAGD
                     MSIIGLCALLALCLVIVGWCSYSLQTVFSARAAESVQHSVRLRSFGHMLRLGLPWHEK
                     HADSRLTRMTVDVDSLARFLQNGLAGAATSLVTMFAIAATMFWLDPFLALTALSAVPV
                     AALATMIYRRLSTPAYAQARLEIGKVNSTLQEKVSGLRVVQSHGQQELEGARLRALSE
                     RFRATRVRAQKYLAVYFPFLTFCTEASYAAVLLVGASQVAAGEMTAGVLAAFFLLLGQ
                     FYGPVQQLSGIVDAWQQATASGKHIDELLATEGTENLGSSSVLPVTGALHLDEVTFSY
                     PDSHEPALNKLTLTIPEGMVVAVVGRSGAGKSTLIKLIAGLYFPTHGNIRIGVQMLDD
                     ASLTEYRRQIGLVDQDVALFSSDIAENIRYSRPSATNEDVEIASQRAGLYEMVCNLPQ
                     GFRTPVNNGGADLPAGQRQLIALARAQLANAHILLLDEATSCLDRTSEERLMSSLTDV
                     VHAGKHSALIVAHRLTTAQRCDLIAVIDKGLLAEYGTHEQLLSAGGLYTRLWHDSVSS
                     TALHRQHNMKEETPG"
     gene            complement(8461..9576)
                     /gene="iroB"
                     /locus_tag="OHN27_003852"
     CDS             complement(8461..9576)
                     /gene="iroB"
                     /locus_tag="OHN27_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin biosynthesis C-glycosyltransferase
                     IroB"
                     /protein_id="HCQ2288217.1"
                     /translation="MRILFVGPPLYGLLYPVLSLAQAFRVNGHEVLIASGGQFAQKAA
                     EAGLVVFDAAPGLDSEAGYRHHEAQRKKSNIGTQMGNFSFFSEEMADHLVEFAGHWRP
                     DLIIYPPLGVIGPLIAAKYDIPVVMQTVGFGHTPWHIKGVTRSLTDAYRRHNVGATPR
                     DMAWIDVTPPSMSILENDGEPIIPMQYVPYNGGAVWEPWWERRPERKRLLVSLGTVKP
                     MVDGLDLIAWVMDSASEVDAEIILHISANARSDLRSLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALHAGIPQIVFGQGADRPVNARVVAERGCGIIPGDVGLSSNMINAF
                     LNNRSLRKASEEVAAEMAAQPCPGEVAKSLITMVQKG"
BASE COUNT         2370 a         2735 c         2702 g         2222 t
ORIGIN      
        1 tatctggcaa ggatgtgagc ttaacgatca aacattttaa gttaggcatt tattagggaa
       61 taggtatgag aattaacaaa atcctctggt cgctaactgt gctcctggtt gggttgaata
      121 gccaggtatc agtagccaaa tcctccgacg atgataatga cgagactctg gtggtggaag
      181 ccaccgctga gcaggtatta aaacagcagc cgggcgtgtc ggttattacc agcgaggata
      241 ttaaaaagac ccctccggta aacgaccttt cagatattat tcgtaaaatg cctggcgtca
      301 atcttaccgg caatagcgcc tcgggcacac gcggtaacaa ccgccagatc gatattcgtg
      361 gtatggggcc ggaaaacacc ttaattttaa ttgatggtgt accggtgacg tcacgtaact
      421 ccgtgcgtta tagctggcgt ggagagcgtg atacccgcgg tgacaccaac tgggtgccac
      481 cggaacaggt tgagcgtatt gaagtgatcc gcggccctgc ggcggcgcgc tacggttcgg
      541 gggcagccgg gggggtggtg aacatcatta ccaaacgtcc caccaacgac tggcacggtt
      601 cgctgtcgtt atacaccaat cagccggaaa gtagcgatga gggcgctacg cgtcgcgcca
      661 atttcagcct tagtgggcct ctggctggta atgctcttac cacgcgtttg tatggtaacc
      721 tgaataaaac ggatgctgac agttgggata ttaattctcc ggtcggtacg aaaaacgcag
      781 ccgggcatga aggggtacgt aacaaagata ttaacggcgt tgtctcgtgg aaattaaatc
      841 cgcagcagat tctcgatttc gaagccggat atagccgcca ggggaatatc tatgcgggcg
      901 atacgcaaaa cagttcttcc agtgcagtta ccgaaagcct ggcaaaatcc ggcaaagaga
      961 cgaaccgcct gtaccgacag aattatggca ttacgcataa tggtatctgg gactggggac
     1021 aaagtcgctt tggtgtttat tacgagaaaa ccaataatac ccgcatgaat gaaggattat
     1081 ccggcggtgg tgaagggcgt attttagtgg gtgaaaagtt tacgaccaat cgcctgagtt
     1141 cctggcgaac cagcggcgag cttaatattc ctttgaatgt gatggttgat caaacgctga
     1201 ccgttggtgc agagtggaac cgcgataagc tcgatgatcc ttcctctacc agcctgacgg
     1261 tgaatgacag tgatatcagc ggtatttctg gctctgctgc ggatcgcagc agtaaaaatc
     1321 attctcaaat cagtgcgctg tatattgaag ataacattga gccggttcct ggcacgaata
     1381 tcattcccgg cctgcgcttt gattatctca gcgactccgg cgggaacttc agccccagtc
     1441 tgaatctttc gcaggaattg ggcgactatt tcaaagtcaa agcaggggtt gcccgaacct
     1501 ttaaagcccc aaacctgtat caatccagtg aaggctatct gctctactcg aaaggcaatg
     1561 gctgtccaaa agatattaca tcaggcgggt gctacctgat cggtaataaa gatctcgatc
     1621 cggaaatcag cgtcaataaa gaaattgggc tggagttcac ctgggaagat taccacgcaa
     1681 gtgtgaccta cttccgcaat gattaccaga ataagatcgt ggccggggat aacgttatcg
     1741 ggcaaaccgc ttcaggcgca tatatcctca agtggcagaa tggcgggaaa gctctggtgg
     1801 acggtatcga agccagtatg tctttcccac tggtgaaaga tcgtctgaac tggaatacca
     1861 atgccacatg gatgatcact tcggagcaaa aagacaccgg taatcctctg tcggtcatcc
     1921 cgaaatatac tatcaataac tcgcttaact ggaccatcac ccaggcgttt tctgccagcg
     1981 tcaactggac gttatatggc agacaaaaac cgcgtactca tgcggaaacc cgcagtgaag
     2041 atactggcgg tctgtcaggt aaagagctgg gcgcttattc actggtgggg acgaacttca
     2101 attacgatat taataaaaat ctgcgtctta atgtcggcgt cagtaatatc ctcaataaac
     2161 agatcttccg atcttctgaa ggggcgaata cctataacga gccaggccgg gcttactatg
     2221 ccggagttac cgcatcattc tgatcgtagt gtttcaggcg tactgcccgc gtaaaagcgg
     2281 gcagtgttta gtggcttaac tcatgacaac ctgctgtgta atttgcgttt tcaccactga
     2341 tatctaacag tgcctggcga aaggaggcat tgaacatcgg cccgtgtccc aggttgggga
     2401 aatcccaaaa tacggcattg acgcctttat ctttcagtat agtgagggtg gtatgaattt
     2461 tcgacagcac cccgacagca tgcgtttccc ggttatcacc ctgtgtcgcc gagccttcca
     2521 ttatcgccag gtgtttggcg aagaattgca gaggctcaac cgccgtaacg cggcttagca
     2581 aagcatcata acctctgccc aacgacgggc tggcgctgta gtacgaccgg aagtaagagg
     2641 aggacagcca ggaatccagc acgaataggc cgccgtagga gtgcccccat aagccgcggc
     2701 gttgccgatc gatattcagt ccctgttcca cttttggggc aatacgcgtt tccagtaact
     2761 ggcggaagtt gttgctgcca ccactcttac ggctaaaacg ccctgagtgg agatctgttt
     2821 ttctgctttc tgctgctggc gtatagtcgt aagccctgct gttgagatcg aaagggaggt
     2881 tggtctgata cccgacagcc acgatcactg gcggtgtttt ttctgacaat tgtttgagca
     2941 gttcgtcatc caggcgatcc ataactgcgt taccgtcaag catatataaa atcgggtaac
     3001 ccgatgccgg tgcggttgta ttcggcacgg ccgtccatac ccgataatgg cgtgtgccat
     3061 cgacagagtc gaaagaggtg acgctgaaat ggtaaaacac ggagccttta tcggctatat
     3121 tcggccccag tggccgcata tccggcttcg cataaacttg cgcactacag ataagggtga
     3181 ggcaagaaag atgaaagaaa atcgctttat gcgggcgtgt tgagcgatac tcgcgggcat
     3241 acatctgatg attattcctt ttccgcaggt gactatttga tttattcagg aagtctgtac
     3301 cgtttcctga aagtgtttgt gggtcaactc aaccctgtag taaaccaatc ccgtcaatca
     3361 gtgcaccgcg ccaccatgcg taatcgtgac caccggtgta gattgcgcaa tggctttcga
     3421 cgccagcggt aatcagccgc tggtgaagct gctgaacgtg aggcactgtc gaaccttcca
     3481 gcgatcccac gcacaggctg atacgtacgc cctgcggtgg ggcagaaagc agatgctcac
     3541 tcacccatga ggtatcggtt tcgctgaaca agcctggtcg actggttctt tctggcgtcc
     3601 accacattga aggagagtgg ctgagcacca gaccgaacgt ttccggtgcg taacgagccc
     3661 ccattagcgc actgatcccg ccgaggctct gcccagccag cactgtgcgc gaacggtctg
     3721 cccactgacg ctgcggttgt tcagcgcgaa tcatcggcag cagatgtccg gcgatatcct
     3781 ttatcagttt gctgcgcccg ccgagtatct cagtgcgttc atgttcatta atgttgtcaa
     3841 tgcccagtac agccacgggc acgatgcgcc cattatttat ggcggcgtca attgccgcgc
     3901 atacgccaag gtgatcaaac caggtttcac catcgggcag cacgaccagc ccgaggggct
     3961 gagaaatatc aacatccgga atatagagcc gaacctggcg agatttcccg gcgataatgc
     4021 gcatggaggt aagcagttgt ccggtgtgga agccgccgtt ccattccgac agggcgggag
     4081 ctttatcaag tgtcagcact gattcctttg cgtttccccg aacgttgatc tccggcattt
     4141 tgtttagcgg atcggccttt ccggcaaggg tggcaaaacg gcctccggac agtgcaatcg
     4201 tctcagccgt agtgccgggg gggatttcca gcagcgaata ggagccgcag taacttgcgg
     4261 gtaaacgcag tgtcagtgtc cagatatccg tttcgggaag ggcgctcatc attccttttt
     4321 ctacgtgctc tttatccgtc acccggttca gacgcagata aacgccctgc agaggatgtc
     4381 gggcgcgcca cagaaaggtc acttctcgct catcttccgc tccttcgact ggcgtcacaa
     4441 gaggaacatt cagcgactca gcttcgcgcc agaagtcagt aaggttagca atgtggcccg
     4501 ctgccagttg gttgcgcagg ctggcgatag cagagggatg ttgttgcatg ttcagcattc
     4561 atactcttta aaaattaagg atgtcagtat aaataattaa agcggctatt cacggtaccg
     4621 ccgcttctga ctgtgttttt aatacattac gtgtccagta actatcccgg ggtttcctcc
     4681 ttcatgttgt gctggcgatg gagcgcagta ctgctgacgc tgtcatgcca taagcgggta
     4741 tagaggccgc ccgcagataa cagctgttcg tgggttccgt attccgcaag taacccctta
     4801 tcaataacgg caatcagatc gcagcgttgc gcggtggtca gacgatgtgc aacaatcagc
     4861 gccgagtgct tcccggcatg cacgacatct gttaacgatg acatcagtcg ttcttcggat
     4921 gtgcgatcca gacatgacgt agcttcgtcg agcagcagga tgtgggcatt cgccagttgc
     4981 gcgcgggcca gcgcaatcaa ctggcgctga cctgcgggca gatcggctcc gccgttattc
     5041 accggtgtcc ggaatccctg cggcagattg cacaccatct catacagccc tgcccgctgt
     5101 gaggcaattt caacgtcttc attggtggcg gatggccgtg aataacgaat gttttctgca
     5161 atatcactac taaacagtgc tacatcctga tcgacaagcc caatctggcg acgatactca
     5221 gtgagcgagg catcatcgag catttgcaca ccgattctga tgttgccgtg cgtggggaaa
     5281 tacaacccgg caatcagctt aatcagcgtc gacttacccg caccgctgcg accgacgacc
     5341 gcgacaacca ttccctctgg gatcgtcagg gtaagtttgt tcagagctgg ctcgtgactg
     5401 tcgggataac tgaacgtgac ctcatcaaga tgcagtgcac cggtgacagg gaggaccgaa
     5461 gaggacccga ggttctcagt gccttctgtc gccagtagtt catcaatatg tttgccgctg
     5521 gctgtcgcct gctgccaggc gtcgacaatc cctgataact gctgcactgg cccatagaat
     5581 tgccccagca acaggaagaa agccgccagt accccggcag tcatttctcc agcggcgacc
     5641 tgcgaagctc ccactaacag aacagcggca taggaggcct cggtgcagaa tgtcaggaac
     5701 ggaaaataga ctgcaaggta tttttgtgct cgcacacggg ttgcgcggaa acgctccgat
     5761 aacgcgcgca gccgggcgcc ctccagttcc tgctgaccat gcgattgcac gacacgcagg
     5821 ccagagactt tttcctgcag ggtgctgttg actttgccta tttccagccg tgcctgtgca
     5881 taagcagggg tactgaggcg gcgataaatc atggttgcca gtgcggccac tggcactgcg
     5941 cttaatgccg tcagcgccag gaacgggtcg agccagaaca tggtggcggc gattgcgaac
     6001 atcgtcacca gactggtggc cgcaccggca aggccgtttt gcagaaagcg ggcgagagag
     6061 tccacatcaa cggtcatgcg ggtaagacgc gaatcggcat gcttttcatg ccaggggagt
     6121 ccaagacgca gcatatggcc gaagctgcgc aagcgcaccg aatgctgaac tgattccgcc
     6181 gctctggcgg agaagaccgt ctgcagagaa taactgcacc agcccacaat gaccaggcac
     6241 agggcgagca gggcacagag gccgatgatc gacatatcgc ctgcggcgac accggagtca
     6301 atgccgtgct gcaacagtat cagtacccct acgcctgccg cagaatcgag tgcgatcagc
     6361 agagcggcga tgacgaacat ccacgccaca ggcttcagta gtgacgtaac tttgcgacct
     6421 ttaccggcca gcgcctgctg tactgcattt tccggtacgc gggtcatacg ggcgacaaaa
     6481 cctttatccc ccgtatccgt tacatcgtct tgtgtcgctg gttcagccgg ccacaattcg
     6541 ttgtggctat tgtgggacgt ggccagaaaa tcgctgtcgc cggtcatcag tgcgcggaac
     6601 gccggacagc gcgcttcaag ttctgccggg gttccggtat ccaccatacg gcccttatcc
     6661 agcaccacaa cccggctggc tagctgaagt gttgaacgcc gtcgggcaat caccagcagc
     6721 atatgccctt cgtcagcata gcgacccagc gcggtattaa tctccgcttc cgtaacagca
     6781 tcaaccgctg aggtggtatc atccaggatt aacacgtccg gtgcagtaat gagcgcccgc
     6841 gccagcgcta ttcgctgcct ctgcccgccg gaaagattcg taccccgttc ggttaacagg
     6901 ctatcgaagc ctttcggcag ggcgttgata aaatcgctgg ctcctgcagc agctgccgca
     6961 cggcgaatgt cgtccgccgt tgcctgaggg tggccatagg cgatattttc cgccaccgta
     7021 ccggcaaaca gaaaagcatc ttcaaatacg atgcctaccc ggcgacgaag cgcctccagt
     7081 ctgatatcgc gaagattttg tcggccttcg ctggtgttga gccatatctt tccggagcag
     7141 ggatcataaa aacgcgccag cagcatcaac agggtcgatt ttcctgaacc cgatgcgccc
     7201 accaccgcca cggtttcacc ggcgcgcagg gaaaaggaga tattgctgag gatatgtctg
     7261 tcgccatggt ggtagtcaaa gctcatattc tccagttcca gtccgacaac ctgactgtta
     7321 atcgactccg tcccgtcctc catctgtgaa cgggtgtcga tcagtgcaaa cacccgctcc
     7381 acggacgcct gagtgcgctg tgcgataacc agaaacgatg ccagtacgcg ggttggcccg
     7441 gtcagcatcg cgaggaagct ggcgaatgca acgaaggtac cgagatcgat ccgcccgtgc
     7501 atgaccgacc acccgccgta gcacagcagg acgatttgcc cgagcaccgg tagcgccagc
     7561 atggtggccc caggcatcgc ctgcgagata accgcaccga tgcgtacacg cacaatctgc
     7621 cgcgactgcc tatcgagcca gtgcgtttca cgcatctctg ccacacagga ttttaccact
     7681 gagatctgtg ccagcacctc gcgtatatgt tcggtgagat tcgccagttg gtccgaggcc
     7741 agcccggttt gcgcgaagac cctacgacgc gcacgaagcg cggtgatcgc caggcatacc
     7801 agtacgcaca cgacgataag cgtcatggcg ggggacatcc acagcatcac ggcaatgccg
     7861 gcaatgtaat aagtgaacac tgccagcggc accgggcaca tctgcagcag ggtatgcacc
     7921 tgctgcagat cgctgttggt ccgtgaaatc acctgcccgg tgcgcagcgc atcctggcct
     7981 gcgccgtcaa gtttctgaat actgtcgaat acccgtcctc tgagggtatg ctgcacccag
     8041 agtgagagca tcccggcata accacggcgc acatagttac cgatgaaatc aaaaaaagcg
     8101 atcaacagta acccgcaggc cagccacggc aggcgggccg gattgcccgc cagtgcgtca
     8161 ttcacagcct gtcttgtcag cagtggcgtc agcgaagcca gcaaaatagt cgatactgac
     8221 gctaccacca cgatgacact aagtttctta cgttcccagc acacgcgcgc caggcgaact
     8281 atccatgact gagccggtgt gggagtgtga ttcgcaggca ttaagcctcc tccgtctgta
     8341 aaatacgatc cactggccgg atcgttccgc aaaaaagcca gcacgctccg gtcccgcatt
     8401 gttatggatg catgactcct agatgggtgc tggcatatga ttatcatgaa ggtgtagtgg
     8461 ttaccctttc tgtaccattg tgatcaggct tttcgccact tcacctgggc agggttgcgc
     8521 cgccatttcc gctgctacct cttcagaggc tttgcgaagt gagcgattgt tgaggaaagc
     8581 attgatcatg ttactcgaca ggccgacgtc gccagggata atcccgcagc cgcgttcggc
     8641 aacaacgcgg gcatttaccg ggcgatcggc tccctggccg aagacgattt gtggaatacc
     8701 agcgtgtagc gctgtcaggg tattgcctgc tccgccatga tgaatgaaac catctgcgcc
     8761 gttgaggaag acacccatgg gtatccagtc aaccaggcga acatttgacg gcagcgagcg
     8821 caaatcggac cgcgcattgg ctgaaatatg caggatgatc tcggcatcga cctcactggc
     8881 agaatccata acccaggcga ttagatcaag gccgtcaacc atcggtttga cagtgccgag
     8941 actcaccagc agacgtttac gctcgggcct tctctcccac catggttccc acaccgcgcc
     9001 accgttatac gggacgtact gcatcggaat aatgggttca ccgtcgtttt ccagaatgct
     9061 catactcggc ggtgttacgt cgatccaggc catatcacgt ggtgtggccc ccacattgtg
     9121 gcgacgatag gcgtccgtga gcgaacgcgt tacacccttg atatgccagg gggtatggcc
     9181 gaagcctacg gtttgcatca cgaccggtat atcatatttg gcggcaatca gcggcccgat
     9241 gactccgagc ggagggtaga tgatgaggtc aggccgccag tgtccggcaa actccaccag
     9301 atggtcggcc atttcttcgc tgaagaatga gaagttgccc atttgggttc cgatattact
     9361 ttttttccgc tgtgcctcat ggtggcggta accagcttcg gaatccagac cgggtgcggc
     9421 gtcaaacacc accaacccgg cctctgccgc tttttgtgcg aattgtcctc cactggcgat
     9481 cagcacttca tgaccgttaa cgcgaaatgc ctgcgccaga gacagcacag gatacaacag
     9541 cccatagaga ggcgggccta caaacaaaat acgcatgaga aaatccctct ccgcttgacg
     9601 atctaaatca ggcagtcgac gcactcatat gccagcagca ggacacgact tttcgttaaa
     9661 cctgctttac cagatgctgc gccatttgtg cttcaccagc gttatggaca gctcagtcag
     9721 agacttttcc gtgacataca caggtgaaca gggaactccg tatccccact tccattaacg
     9781 cttacgcgcc attgtcctgt tgaacagccc aaatggcgtc ggacagctac gccgggatgt
     9841 tctgacgcat ttgtgcagtg ttagaccgaa caacgaatct ggaaagaacc gtgatttctt
     9901 agacacacga tgcatcatcc tgatagtctc gccttcgctg gtctgtaagt aaaaacgccg
     9961 ttgtacaggc gatgtttgtt ccctttcagc cttaccagaa acaacacaga cacaaatgct
    10021 aatgataat
//
LOCUS       DAJOSY010000139        10029 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJOSY010000139 DAJOSY010000000
VERSION     DAJOSY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.139"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            127..558
                     /gene="osmC"
                     /locus_tag="OHN27_003853"
     CDS             127..558
                     /gene="osmC"
                     /locus_tag="OHN27_003853"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCQ2288218.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(614..1540)
                     /locus_tag="OHN27_003854"
     CDS             complement(614..1540)
                     /locus_tag="OHN27_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2288219.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(1533..2519)
                     /locus_tag="OHN27_003855"
     CDS             complement(1533..2519)
                     /locus_tag="OHN27_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2288220.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIDLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(2516..3412)
                     /locus_tag="OHN27_003856"
     CDS             complement(2516..3412)
                     /locus_tag="OHN27_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCQ2288221.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTSAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3409..4431)
                     /locus_tag="OHN27_003857"
     CDS             complement(3409..4431)
                     /locus_tag="OHN27_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2288222.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(4433..5983)
                     /locus_tag="OHN27_003858"
     CDS             complement(4433..5983)
                     /locus_tag="OHN27_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2288223.1"
                     /translation="MKRSISFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGIIVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(5997..6578)
                     /gene="ddpX"
                     /locus_tag="OHN27_003859"
     CDS             complement(5997..6578)
                     /gene="ddpX"
                     /locus_tag="OHN27_003859"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCQ2288224.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            complement(6836..9235)
                     /gene="dosP"
                     /locus_tag="OHN27_003860"
                     /gene_synonym="pdeO"
     CDS             complement(6836..9235)
                     /gene="dosP"
                     /locus_tag="OHN27_003860"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCQ2288225.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEIQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDLGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(9260..>10029)
                     /gene="dosC"
                     /locus_tag="OHN27_003861"
     CDS             complement(9260..>10029)
                     /gene="dosC"
                     /locus_tag="OHN27_003861"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCQ2288226.1"
                     /translation="LGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISRLIQDFDGIF
                     NQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRR
                     FLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVR
                     SSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSIGAA
                     MFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL"
BASE COUNT         2479 a         2603 c         2533 g         2414 t
ORIGIN      
        1 tgtgggaatt taatttaagt gcagaagtaa tattttcgcc ggattttatt cggaatatcc
       61 tgcttatcct cgtgctgttt ctcacgtagt ctataatttc ctttttaagc ccacaggaga
      121 gcaacaatga caatccataa gaaaggtcag gcacactggg aaggcgatat caaacgcggg
      181 aagggaacag tatccaccga gagtggcgtg ctgaaccaac agccgtatgg atttaacacg
      241 cgttttgaag gcgaaaaagg aaccaaccct gaagaactga ttggcgcagc gcatgccgca
      301 tgtttctcaa tggcgctttc attaatgctg ggggaagcgg gattcacgcc aacatcgatt
      361 gataccaccg ccgatgtgtc gctggataaa gtggatgccg gttttgcgat tacgaaaatc
      421 gcactgaaga gtgaagttgc ggtgccgggt attgatgcct ctacctttga cggcataatc
      481 cagaaagcaa aagcaggatg cccggtctct caggtactga aagcggaaat tacgctggat
      541 taccagttga aatcgtaaag cattgccgga tgacgcgtca ggcgcgtgaa tgcctgatgc
      601 gttgttagca tctctacagc gcccgccagc accggagctc acgtccgtcc tcccttattg
      661 ctaatgattg ccggacttca catccgtggg ttgccagcgg gcaacgttcg tagaaaaaac
      721 agccttgcgg caacgtgcgg tttcccggca gatccgtttt acgtaatgcc cattcttcct
      781 ccagcggttt atcaatggcg gggagggaat ccagcaataa tcgggtgtat ggatgtgcag
      841 gtgccgtcag cacctgctgc gcgtcgccca gttctacaat ctgcccgaga tacatcaccg
      901 ccacccgatc gctcatatga cgtatcaccg agacattgtg tgaaatcagc acataggtca
      961 gcccgtgatt ttcctgtagc gttaccagta aattgaggat ctgcgcctga acggagatat
     1021 ccagcgcaga ggttggctca tcaagcacaa tcacgtcagg ctgcgaagag agtgctctgg
     1081 caatggcgat acgttgccgc tgcccgccgg agaacgcatg aggcaggcgg tcgagatact
     1141 ccggacgaat acccacctgc acagccaact cctctgccaa cgctcgccgc tgttgttcac
     1201 tactacgctt agctatccag agcggttctg tgatgatgcg ccacaccggt aagcgcggat
     1261 taagcgaaga gagcgggtcc tgaaacacca tctgcataat gcgttgtgag ccagaacgga
     1321 tgtactgccc gtggctcggt tgcagcatac ccattaaaag ctgtgcgagg gtgcttttgc
     1381 cgcagcctga ctcgccgacg atccctaagg tttcaccacg gcggatctgt aaatcaatac
     1441 cattaatggc atgaacatgt tccgtagttt taccaagcca gtttttacgg gccgggaaat
     1501 tgatatggac gtcgcgtaac gttaataacg tgtcagacac ttataacctc ctgctgcgga
     1561 taccaacagg cacagcgctg gttgttgtca ccacacgctg tcagcgccgg gacgttttca
     1621 cactgtgcac ctgccgcata gcaacgatcg cgaaaagcgc agccgtcagg caaatgggtg
     1681 aggtttggta ccgtccctgg aatggcgggt aatagctggc gtggtactcc atgttctggt
     1741 gcgcattgca gcaaaccaat ggtatacgga tgccggggat gatggataac gtctgccgtc
     1801 acgccgcttt caattacgct tccggcatac atcacgtaaa cgctatcgca cagttgcgac
     1861 accacggcca tatcatggct gatgaacaga accgcagttc cactggcgcg ggctttatgt
     1921 ttaagcaaac gcagtacctg caactgcacc gtgacgtcca gcgccgtagt cggttcgtcg
     1981 gcaataatca attgcggctc gcaggagaat gccagcgcaa tcattacccg ctggcgcata
     2041 ccacctgaaa gctcaaacgg atagcgcgac ataacttcca cggcatccgg gatttgcatc
     2101 tcttccagca ggtcaatcgc tttagctctg gcttcccgac gacttattgg ttgatgatgg
     2161 cggatcacgt ccatcatctg aagacctatt cgacgtgtcg gattgagggc ggtcatcggt
     2221 tcctgaaaga tcatcgccac tcgtgcgccg cgccactgac gaagctgttt ttcccgcgcg
     2281 ttaagaacat cttctcccag cagggaaatc tgcccccgat gtacgcaata actgcccgtc
     2341 ggtagcagac gcataatcag cattgcggtg actgatttac ctgagccgga ttctcccacc
     2401 agaccgacaa tttcaccgcg gttaatctgc aaggacacat tgttgagcgc gtgaacatca
     2461 ccgttaaaac cggggaaact caaatgcagt tgttgaatgt ccagaacggg ttgggtcatg
     2521 actgctttcc tcctgctttc gggtcaagca gatcgcgaat accatcacca aagagattaa
     2581 acccgacggc ggtaagcaaa atcgctgccc ccggaaatgc gcaataccac cactgatcga
     2641 gtacatagtt gcgaccattt gctaccatcg cgccccattc agcactcggt tgttgagcac
     2701 ccaggccaat aaatcccaac gtggcggcca ttaaaatcgc gctaccgata tccagcgatg
     2761 cctgcacgat cagcggcggt agggagttac gtaaaatatg ccagttgatc agatgccaac
     2821 gagacgcgcc aaaggttttc gccgcctgaa cataggtata ctggcgtaca actaatgctt
     2881 gcccccgcgc caggcgcaca taaaagggaa ttcgcacaat agcaatagcc agcatggcgt
     2941 taaacaaact cggcccgaga gcggcagcca gtgccattgt cagtaccaac gaaggaatcg
     3001 acagcataat gtccatgatg cgcatgatga tggcgtctgc gcgtccgcca agcacaccgg
     3061 atagacatcc gagtagcgaa ccaatcatac ccgcaatggc aaccacgact aatccggcga
     3121 gaattgattg ctgactgccg accagtacgc ggctgaacag atcgcgtccc acttcatcgg
     3181 tgccaaacca gtgcgccgca gaaggcggca aaaggcgggc ggttaaatca atggcgttgg
     3241 gatcatgcgt cgccagccac ggtgaaaaaa tcatcatcaa tagcattaat acaatgatga
     3301 ctgcgctggt cacggtgagc ggactgcctt tcagcatcca taccagtttt gcaccgttaa
     3361 atcgcgtttg tttttgtggg cgtacggcgg acgtttcctc gcttagcatc attcaccacc
     3421 tccacgtccg atacgcggat caatccacaa atagagcaaa tccaccacca ggttgaccag
     3481 cacataagca aatgaaacca cgacggcaaa gcccatcact gccgggaagt cgagcgcctg
     3541 tattgatgtt actacccatg cacccattcc gggccaggca aaaacggttt cggtgagcac
     3601 tgcgccatac aacaaatcgc ccagcgccaa acccaatacg gtaatcgatg ggatcaacgc
     3661 attgggtagc gcataacata aaacgatata ccagccgggc aagccgctgg cccgggcggt
     3721 acgaatgtag tcttcactca attgttccag catcgctgag cggatctggc gagcgacaat
     3781 tcccaggtga acgaacgcca gcgttaatgc cggtaagatg agatgttgca acgcattgaa
     3841 gaagacttca ccgttgcctt caagcagcgc atcgagcaga taaaagccgg taacgtgcgt
     3901 tggtggatcc agccagtcat caagccttcc gccgccggga agaatttgca gatgaccata
     3961 aaacagcaca atgacgccca gcccaagcca gaacgcaggt gtggagattc cggtaatggc
     4021 cattattcgc accagatgat ccagccagcg atttcgccag actgcagaga gtatgcccag
     4081 cgggatgcca atcaggagtg ccagcagcag ggcaccaaaa gccagctcca gcgtcgccgg
     4141 gaaaaatata cgcaactctt ccagcaccgg acgcccggta cgaatggatg ttcccaggtc
     4201 accatgaaac aggtcgctga cgtagcggta aaactgtaca tacagtggct ggtccagtcc
     4261 cagttgctgg cgaatatttt ccacgatagc atcgctggcg cggtcacccg ccagtaaccg
     4321 cgccggatca ccagggatca gatgcgagat aataaacgta atcacgcaga cgcccgccac
     4381 cacgagcacc agcccccagc agcgttggcg taaaatactc cagaaggtca cgttatttac
     4441 tcatggtatt gatattgaag acctgttcca gcatgggatt gaacacaaag cctttcacct
     4501 ctttgttcat cgccagttgg tagtttttct ggaacaggta cacataagca gcgtcatcaa
     4561 tgacgatttt ctgtgcctgc tggtagtccc gggtacgctg cgtctggtcg gtggtcgcaa
     4621 gcgcattgcg cagtaactta tcgacctcac tgttttcata gaacgagcgg ttacccggca
     4681 gacctttttt gtctgactca aaccagtaat tcataaacat atacgggtcg gcaaaatccg
     4741 gactccagtt gccaatcgca atgtcgtaat cacctttacc cactctgtcg cgcatggtgg
     4801 cgttcgccag cttttccagc ttcacaatga tgcccagctt gttgagactg gattgtgtcg
     4861 ccagagcaat aggctcccag ttcggatcgt tatcggagta gagaaacgtc aggctggtgg
     4921 gtttgctcgt cactttatcc cattcggctt tggctttcgt ttcgtcatgg ttgtattgca
     4981 ttgccgtcgc atcgtagccc cacatgcctt ccggaatcgg gccgcgcatc tgttttccgt
     5041 taccactcag aatgccgtta accatgccct gataatcggt agaccaggaa atggcccgac
     5101 gcagatccgc ctgattaaga ggcgctttgc tgttattcag atacagatag gtaacgcgca
     5161 gtgacggata ctctgccaca ttgactttat tttcctgctt cagggcgttg agttgatcca
     5221 ccggcagcgc atcggcaatg tcaatgtcgc cacgggagag ctgcaggcga cgggaggcac
     5281 tttcaccaat aatttttacc gatacccgtt tgaagttcgg tttattgcct gggtaatgcg
     5341 gatttggcac cagaactaat tgctgacctt tttgccagct tttcagcata aatggtccgg
     5401 aaccggcggt attttgcgcg aggaagccgc gagcatcatc cgctgcatgt tcctttaaga
     5461 ccgccggatt gataatggat gcaccgtcat tcgccagcgt gtagaggaac ggtgcgaatg
     5521 gttggctaag ggtaaacttc accgtatgtt cgtcgggagc atcaatcttt aaatctttgg
     5581 gaaatgcttc tgctggcccc tggccgattt ttagtagccg ctcaaaagaa agttttactg
     5641 cttcggcagt gacaggtgtg ccatcggcaa atttagcatt atctttcagg gtgaacgtcc
     5701 actctttttg atcgtcagac gctttccagc tacttgccag atcgccttca acgtcggttg
     5761 agcctttatc accgtccgtt ttgtactgaa ccagccgctg ataagacggg taggtcactg
     5821 tccagtcgtt attatctatt gttaccgccg ggtcgagggt ttgtggatcg gcggccttac
     5881 caatcaccag catatctttt ggtacggcgg cgtgcgcaac cgggaaattt gtggcaagga
     5941 cgagcgcgag caatgtggga cgaaacgata tcgatctctt catgacagtt tcctgattaa
     6001 ctgacgtgct gtgtgccagg ggatataaaa caagagaatt gatcagcgag cagagggtaa
     6061 ctcgctgcct ggggtaattc gaagtgccac cattcgctgg agataccgac aaaaccgcca
     6121 ccagtcatta tcgcattcag caacagccga ttgcgctgag cggcgggcgg gacggaaggg
     6181 tgataggcat gggagcgctc gtgcatttca tcgaaccctg cgcccatatc gaggatgttc
     6241 ccgtgctcat cacgaagcgt caggtcgatc gccgtgccac ggctgtgatt agaaccgacc
     6301 gtcacatcaa caacatattg cgggtctggg caggcttgcc acaacatcgc ctgtgcttgt
     6361 tgtgggcgat acgcatcgta aatcaccagt tgtaaccctg acagctgggc gatgctgata
     6421 cttttcgcca gcgcggtaat cgcatccttg tgtaacagac aacgcgcttg ctgataaata
     6481 gctttacctg tgatgttatc agcgcaggcg tatttcaatt cgatctccag atcagggaag
     6541 attacggcta aatcaaccag ttcggtggta tccgacataa acggttcctg tataagacaa
     6601 aaattgctgc gctttccgct tatgcagatc tcatgccatg ccgggataag cgccagaatg
     6661 ctggcttaaa gttatatttt ttaacttttg atcaacattt gtgcagcgta gtgcagtttt
     6721 ggtgcaagag gggaagttaa ggaaggaatc tcccggaatc gtagctgaaa tcacagtatt
     6781 taagtgacag tgtcacgtta aatgaaaacc cgcgagtgcg ggcgagagga atttgtcaga
     6841 ttttcagcgg taacacgctg ctcatccagc ctggaatttc ttcggcgggt agggggcggg
     6901 aaaagaaata tccctgaata acgcgacagt ggatcttgcg tagcatctca aattgctctt
     6961 tggtttcgac gccttccgcc acgacggtta aattgaggct ttgcccaatg ctggtaatgg
     7021 cttcaagtaa ggcaaggatg cgtttttcgg tcagacaacg atcgacaaaa cttttgtcaa
     7081 ttttgatttc cgttaccgga agactgacta agcgggataa tccggaaaag cccgtaccaa
     7141 aatcatctac cgataagccc acgcccatat cacgcaggat ctgaatgcgc ttaaagattt
     7201 cggtatcgtg ttccatcatc atgctttccg tgatttctac cgtcagctgg tggccgtcaa
     7261 taccccaggc gtgcattgca tcagacacct gattaggcag ttgattactg cgaaagtgca
     7321 gcgccgacaa gttcacggat aacgccggga tatgaatatt ctggctacgc cattctgcta
     7381 actgacggca agcttccgcg atgacccagc gcccaatatt ttcgatttca ccaatctctt
     7441 ctgcgagagg aataaaccgt gaagggggca catgaccatg caggggatcg tgccagcgag
     7501 caagggcttc gatgccgtac agttcacccg tttctgcgaa gatttgcggc tggtaaacca
     7561 gtttcagttg gttattgcta atcgcttctt tcagcgctgc gcctaaaacc aaacgctctt
     7621 ttaccatttc gttcatcgcc gggctgaaga actgccagcc gttaccgcca ttcttgcgaa
     7681 tataatccat tgcattgtga gcagtggaga gcaagtaatc gcggttttta cccaggtcgt
     7741 agctgatgcc aatactcaag gtaagcggga agggtttatc gtcaatcatt atcggcttgc
     7801 tgaccacatt ccgtagctca tcggcgattt gggtaatgtt actgacgtcg ttttcgaggc
     7861 tcacgaggac aaactgcgta ccttcgatac gacagagata ctgatccggt ttgagttttt
     7921 cacgaaagcg attgaccact tccagcaatg cctgatcggc ccacgcatag ccaaggctat
     7981 caatcacatc ctgaatatgg tcaacaccga tgagatacac cacgggagag acggctttgt
     8041 cgaccaggtc atcgaggtaa ttgtgcaggt tattgcgatt tggcagaccg gtcatcggat
     8101 caaattggat gagttgttca atatgctgac ggcttttttc ctgttccagc gccagcgcgg
     8161 ccatatgctg gctgatatct gccacgcgtt cgataaaggc gctggtttct gctcctgacg
     8221 aggttttaat ttgcaggatc cccgcaggcg cgccatcacg ctgacgaatg gtcgctgacc
     8281 agctttgcgc attttgaatt tctgcaccgt gggaagatga cgcccagtgt atcggcatcc
     8341 cgttgcgcag tgcgaacagc gaaacatgcg attcgttgag tacagattcg atgttacgac
     8401 aaatgatttc ccccatttca tgaaatggcg ggctgctgca catggcggcg agaatattgc
     8461 cttcaagctg gcgaatctgc cgttcttcgg tgatatccga gaaagtcatt accaggttct
     8521 gcagatgcgc gagcacgtca taaaccgggc tgatagaggc tttaatccag attttttcac
     8581 cggtgcgcgt caacagcaga aattcgtcct gatcgcgggc ggttttccat agcaactgtt
     8641 gtaaacgaat gcggttatcg gcagggaatt caggaatgtt caggagtgta tcgggctgca
     8701 taccgctggc ttcgctaatg cagtaaccaa acatttcggt aaatgcgcga ttgcactgca
     8761 caatatggcg ttccggatcg aggacaatca ccggtcggtc gagatggtca acggcaataa
     8821 tcaattgtcg ggtctgttct ttttgcgcca tttctacgct ggcatcccgt accagcgcca
     8881 ggtaataaac tttcccctcg gcgctcactt tcgatagcgc aaaacgggtc cagattttac
     8941 tgccgtcttt tttctccagc tgcagctccc gactcatccc ctcaacacgc gctttaccgc
     9001 cttcacggtt gtgacgaatg tattcaggat gcgcaggacg caaatcccgc ggaatcagca
     9061 tatcaatgtt attgccaatg acttcttcac gtttgtatcc ccagagcttc tctgcggcgg
     9121 ggttgaaaaa catcacttca tcattttcgt taattaacac cgcacccatc atattttgct
     9181 caagggcggg gaaaaaaatg ccatcggcgg cattatccgc atcggttagc ttcatgatta
     9241 cctctgcatc ctggcgcatc taaagactgg ctttccagag ttcaacacgg tttctacctc
     9301 gtcttttggc gatatacaga gcttcatcgg ctatttgaat gaggcgctca tagtcaggat
     9361 gaccattaaa catggcggca ccgatggaaa gtgagagggc aatatcttcg ccgtttgcgg
     9421 ctttcagttt ggttttctcc acccgactgc gaatacgttc tgcggtacgt aacgtttcgt
     9481 tttcagaagc ttcagtcaaa acaatgataa attcatcgcc cccgtagcgg aaaacataat
     9541 cactactgcg gacgttgtca taaaaggcct gagagacttt acgcagaatt tcatcaccag
     9601 tgttatggcc ccacgtatcg ttgatctctt tgaatttatc aacgtcaata atcagcactg
     9661 acagcggtgt accggtccgg ttggcatggg caatttcgcg tttgaagata gtcggtagga
     9721 aacggcggtt aagtaatttc gtcagtacat ccataccgac ttcgtggcgc gatacttctt
     9781 caaacaattc acgcagcaag gtaataattt gcgatacggt atttcttatc tgtaataaaa
     9841 atttcacccg cagacttctg ttattcaaat ttctggtgtt acgcatggtt tgattgaaaa
     9901 taccgtcgaa atcctgaatc agacgggaga tatggcctac ttcggcaata ccactaaaat
     9961 aatgtcgacc tttatggtta aaccacaggc caaaatcagc ctggcttaaa ggcaaactac
    10021 tgcctaaat
//
LOCUS       DAJOSY010000140        10000 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJOSY010000140 DAJOSY010000000
VERSION     DAJOSY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.140"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            7..82
                     /locus_tag="OHN27_003862"
     tRNA            7..82
                     /locus_tag="OHN27_003862"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:Thr,seq:ggt)
     gene            122..237
                     /gene="rrf"
                     /locus_tag="OHN27_003863"
     rRNA            122..237
                     /gene="rrf"
                     /locus_tag="OHN27_003863"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1226)
                     /locus_tag="OHN27_003864"
     CDS             complement(468..1226)
                     /locus_tag="OHN27_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCQ2288227.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEDLAVHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1234..2337)
                     /locus_tag="OHN27_003865"
     CDS             complement(1234..2337)
                     /locus_tag="OHN27_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCQ2288228.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAALAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQYLEKRFNTGRTPH"
     gene            complement(2347..3528)
                     /locus_tag="OHN27_003866"
     CDS             complement(2347..3528)
                     /locus_tag="OHN27_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCQ2288229.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3596..4621)
                     /locus_tag="OHN27_003867"
     CDS             complement(3596..4621)
                     /locus_tag="OHN27_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2288230.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5052..5273)
                     /locus_tag="OHN27_003868"
     CDS             complement(5052..5273)
                     /locus_tag="OHN27_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288231.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5526..8630)
                     /gene="acrF"
                     /locus_tag="OHN27_003869"
     CDS             complement(5526..8630)
                     /gene="acrF"
                     /locus_tag="OHN27_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCQ2288232.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEAQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8642..9799)
                     /gene="acrE"
                     /locus_tag="OHN27_003870"
     CDS             complement(8642..9799)
                     /gene="acrE"
                     /locus_tag="OHN27_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCQ2288233.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2652 a         2584 c         2410 g         2354 t
ORIGIN      
        1 tagcgtgctg atatggctca gttggtagag cgcacccttg gtaagggtga ggtccccagt
       61 tcgactctgg gtatcagcac cactttttag gttaaagttc ggcagattag aaaagaattt
      121 gtctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg
      181 ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccagacatc
      241 aaataaaaca aaaggctcag tcggaagact gggccttttg ttttatctgt tgtttgtcgg
      301 tgaacactct cccgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
      361 ggcccggagg gtggcgggca ggacgcccgc cataaactgc cagacatcaa atcaagcgaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg caaaaaattc atcaggtgca
      541 gcttgctcca ctatttcccc acgatccata aaaattaccc ggtcagcgac ggttcgtgca
      601 aaccccatct catgtgttac acacaacatt gtcatacccg actgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctcagga tcgagcgccg acgttggctc atcaaacaac
      721 ataattttcg gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacct
      781 gaaatctgtc cgggaaactt atgcgcatgt tcggcaattc tcacccgctc taggtaatgc
      841 accgccagat cttcagcctc tttcttaggc atcttgcgta cccaaatcgg tgccagggta
      901 cagttctgta aaacggtcag atgagggaag agattgaaat gctgaaagac cattcccact
      961 tcctgcctga cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctaccacg
     1021 atccgtccct gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg
     1081 gaacctgaag ggccacacag aacgatccgt tctcccggtt gcacggttaa atttatattt
     1141 ttcaaaacat ggaattgtcc ataccattta ttgacgtttt ccagcgtaat catcgcgtta
     1201 gcaggttgca gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa
     1261 acgtttttcc agatactggc tatagcgcga catgctgaaa cagaagatcc agtagatcag
     1321 cgcggcgaaa acataccctt ccgtcgacat acccaaccag gcgggatcaa cggttgcctg
     1381 ctgaacgcta ctgaaaagat cgaacaaccc gataatgatc accaggctgg tatctttgaa
     1441 gagtgcgatg atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat
     1501 aaccagcccc tgagttttcc agtaacccaa cgccagcgac tctgccgctt catattgccc
     1561 tttaggcagc gcctgtaatc cacctcgcac gacttccgca acatatgctg actgaaacag
     1621 gatcacgcca accagcgcgc ggatcaattt gtcgatactg gtgccttctg ccataaacaa
     1681 cggcagcatg accgaagaca taaacagaac ggtaatcagc ggcacgccgc gccagaattc
     1741 gataaaaatg accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag
     1801 taagatcccc cacggtagcg cccccgcaat cccaactgat gcaataatta aagttagcgt
     1861 cagcccgccc cattgccggg tttcaacccg ctcaagagca aaaaaaccgc catacatcag
     1921 ccaccagaca atcagtgggt aaatcaccgc caaggccgca atatagcgac cgcgatgcgg
     1981 gagtattttc cagaacattg gtgcgatcga cacaagccca atcagtaatg cgaggttaat
     2041 tcgccagcgt tggtcgtgtg ggtaaagccc atacataaac tgaccaaatc gttcgtggat
     2101 gaagacccaa caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccagttagc
     2161 ctgcaaaaat gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc
     2221 aatagtcagc aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc
     2281 gcggcttgag ttatggctcg ccgggcgcgg gggatgagac agcaatactt ttgtcatcat
     2341 gactccttag cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat
     2401 agtcaggctg ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc
     2461 cgtctgattc agcacggtgc cggcaaacag cgaaaccata tcgggatagc caatagcggc
     2521 ggcaagagag gagtttttga cgatgttgag atactggctg gttaacggtg gaataatcac
     2581 ccgcaatgcc tggggaataa tgacctggcg tagcgtaacc gggttgggta atcccagtga
     2641 ccgagccgct tcatgttgac cataaggcac tgcctggatc ccagcgcgga taatctcggc
     2701 gataaatgca gatgtataaa ccgaaagtgc cagcgttaag gctgccagtt caggaattaa
     2761 aaccatcccg ccgcggaagt taaagcctcg tagggctggg acatcccagt gtagtgctgc
     2821 gccaaaaagc cattgcgcca gtaaaggcaa accaatgatc aacactgcgg cgatcggcca
     2881 ggtgcggcgc agttgtccgg tctttatctg gtatgtttta ttaaagcgga atagcccaac
     2941 agaaaggact atagccataa caacagccag gataaacgca ataaatccgt ctcccagctg
     3001 cggtgacgga atataaagcc cacgattgct caaaaaggcc agatcaaacg cgctcacagc
     3061 ctggcgtggt ccgggcaaat tgcgcaacac ggcaaagtac cagaagaaga tttgcagcag
     3121 cgggggaata ttacggaaga tctcaatata aattgtggaa agctttcgta gcagccagtt
     3181 atccgaaagt ctcgccagac cgataaagaa gcccaaaaca gaagcgaaca caatacacaa
     3241 tgcagaaacc agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc
     3301 ctgctggtaa tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc
     3361 aaaacctgaa gtaatgccac gattattgag attcgttaca gtgttgtgaa ataaccaacc
     3421 aacaatgccg acaacagcaa caacggcaag gatctggaat aaccaggcgc gaaccgtagg
     3481 gttggcaaaa gagagtgagc ctttaacggt tgagcggcga tgagacatag aaacctcgga
     3541 aagcataacg ctgaagagag cgccgccgta gcgacgctcg ttacctcaca ccgacttaac
     3601 gcacgggcgg tgcgtactga ataccgccgt tattccagag attattttgc ccacgtttaa
     3661 ttttcagcgg gctttctgaa cctacgttac gctcaaaaat ttccgagtag ttacccacct
     3721 gtttgatgat gttataggcc catttattat ccaacttcag atccttgccg taatcgcctt
     3781 ctttacccag cagatgtgcc atatcaggcg ttgctggatt agccgctttt tcatcgacgt
     3841 tctgggaatt gatgcccatc tcttcagcat ttagcatggc gaaaagcgtc cagcgtacaa
     3901 tcgagaacca ttcatcatcg ccacgacgaa ctaccggccc aagcggttct ttagagataa
     3961 cctccggtaa gacaatccat tcagccgggt tgcttaattt gatgcgcagg gcatacagtt
     4021 gggattgatc cgaagccagc gtatcgcagc gaccagattc cagtgccttc gctgattcgt
     4081 cagagcgatc gaaagtcact ggtgtgtact tcatattgtt tgccttgaaa tagtcggcga
     4141 cgttgagttc ggtatcagta cccgcctgaa tacagacggt agcgccatcc agttctttcg
     4201 cgctttttag ccccgcttta tcgtgcgtca ggaagccaat gccgtcgtaa taagtgacgc
     4261 cagtaaatgc cattcccatc ccggcatcgc gagatgaagt ccaggtcgta ttacgggaga
     4321 gcaaatccac ctcccctgac tgtaaagcgg tgaagcgttc ttttgcagtg agcggggtat
     4381 atttcacttt cgtgtcgtca ccaaatacag cagcggcaac accacgacaa atatcaacat
     4441 caatacctga aaatttaccg tcagcatcgg cataagagaa cccaggtaat ccatcactga
     4501 tcccgcattg cacaaaacct tttttctgta cggcatcaag cgtcgcgcca gcatgcgcct
     4561 gatttgcaac ggcaagcagc acgctggcgg cagccagtgt ggctatcatc atctttttca
     4621 taatgcttcc tgtgaggcga aaattatcgt tataggacgt ttgggaaacg tcttttacct
     4681 gtctgtggcc atcctcttat aagcaaaagt agtgccagct tttgcgcacg atttcgcgtc
     4741 gcaataagct gagtgtagac aggaaataat agaaacagcg ccccgcaatg gtgcgaaaat
     4801 gaaacatgcg ccacattttg gcgcaaaata tttacgcgtg ttttactaag cagaataatg
     4861 attgttagcg tgagtatatt tctggtggga agtgcgaaag ttaaattgat gcgttatttc
     4921 gaaagcagcg aaataacgcg atataacatg ctaagtcagc agagaattag ccagcagaat
     4981 atagcaatat tttatcttat gtaaagatac tgtatcgtta aacagagcgc gaaactccgc
     5041 gccctgttca gttatttcga cagagcgatg atccccatcg tcaggccagc aacagctgct
     5101 gctccgccag caatagcccc agccgtttcc cagttatcct gagttgtgcc tttcatacag
     5161 gtattggtgt gaaccagacg cccctgatcg tcataaaccg gaacgcaggg cgaatcgtga
     5221 gcacagcccg ccagcaatgc ggtgagcaat gccacaggaa ttaatctttt catggtgtta
     5281 cctcagtaat aaacgctaac gcttaataag ttaactctct tattaacgag ctgaatgcca
     5341 ttttatcacc ggaggttata aatcttgcgg aatgaataat gacaaattat gtgaattgta
     5401 aaaatagtga agggagcggg aactatagaa agcttttaaa gagtgagcaa aagaatatct
     5461 tattaatttt tatgggtttc actgaaaata ataaaggcac ccgaaagcgc ctttatgttt
     5521 cagatttatc ctttaaagca acggcggatc accacaaaga acaccggtac gaagaagatt
     5581 gccagcaacg ttgcagagac cattccgccc attaccccga tacccactgc gttctgcgcg
     5641 ccactgccgg caccgttact gatagctagc ggtaatacgc cgagaataaa ggcgagagag
     5701 gtcatcagga taggacgcag acgcatacgt actgccatta gtgtcgcttc aacaacacct
     5761 ttaccctctt tctccatgag atctttagcg aactcaacaa tcaaaatagc gtttttggcc
     5821 gacaagccaa ttgtcgttag caagcccacc ataaagtaga cgtcattttt ttgattaaag
     5881 agtgttgccg ccagtagcac gccgacaatc cctaacggca ctaccaacat aaccgagaca
     5941 ggaattgacc agctttcata gagtgcagca aggcacagga aaacaaccac aaaggaaatt
     6001 gctaccagag cgggagcctg gtttcccgat aagcgttcct gatacgacat acccgtccag
     6061 tcataaccaa tgcccgcagg taattttgac gcaaggtttt ccatcaacgc catggcatcg
     6121 ccggaactgg ttcctggcgc ggcttccccc tgaatctcca ttgacggcag accgttgtag
     6181 cgttccagtc gcggagagcc atacacccaa tgtgaagtgg taaaggccga gaatggcacc
     6241 atttcgccgt tggcgctgcg gacataaagt ttatcgacat cttctggcag catacggaat
     6301 ttggcatccg cctgaacata cacctttttc acgcgaccac ggtcgatgaa gtcgttaacg
     6361 taagtcccac ccagcgccgt tgaaatggtc tgattgatgt cagaaagtga aacacctaat
     6421 gcctgcgcct tttcctggtc aacttccagt ttaaactgcg cggtgtcttc caggccatta
     6481 gggcgcacgc tgactaagct ggcaggatgt tgcgccgcca taccaagcaa ctggttacgg
     6541 gcctgggtta gggcatcgtg acccagccca gcctgatcaa ttaactcaaa gtcgaaaccc
     6601 gttgccgtgc ccagttcaac aatggctggc atgttgaatg gaatgacaaa accgtcgcgg
     6661 atcttgccca attccatttt ggcacgatgg attaccgctt ccgcactgtt ttcgtcacca
     6721 ctacgctctt cccacggttt cagactgacg aaggccatac cggcgttttg tgcctggccg
     6781 ctgaagctaa agccgttaac cgtaaagaca ctttcaacgt tcgctttctc gttcttcaga
     6841 tagtaatccg tcacttgatc caacactttt tgcgtccgct cttgcgtcgc gccagcgggt
     6901 aactgaatca tggtcagaaa gacaccctga tcctcttcag gtaagaagga agacggaaga
     6961 cgtaaaaaca acaccaccat tcctgcaaca atcagcgcat agatcagtaa atatcgtcct
     7021 gtggaaccga ggattttgcc gacgctgttg gtgtagtggt taacgctatg atcgaaggta
     7081 gtattaaacc agccgaagaa accgccctta ttttcgtgat gctcagcaga gacgggttta
     7141 agcagcgttg cacataacgc aggggtaaga atcaatgcca ccagaacaga aagcgccatt
     7201 gccgaaacga tggtgataga gaactggcga taaattgccc cagtagaacc gccgaagaat
     7261 gccatcggaa taaataccgc tgacagcacc atcgcgatac ccaccagtgc gccctggatt
     7321 tgcgacattg atttttccgt cgcttctttt ggcgggagct tatcctccat catcacgcgc
     7381 tcgacgttct ccaccaccac tatcgcatca tcgacgagca gcccgatggc aagcaccatc
     7441 ccgaacatcg ttagtgtgtt gatggagtaa ccaaaagcag cgaggatggc aaacgtccct
     7501 aacaacacca cgggtaccgc aatggtgggg atcagcgttg ctcgcatatt ctgcaagaac
     7561 agatacatca ccaggaacac cagcataatg gcttcgaaca gcgtttttac cacttcgtga
     7621 atagaaagct ggacgaatgg cgtggtgtca taagggtaga gaaccttcat tccctgcggg
     7681 aagaatggct gtaattccgc cagttttgcc ttaatggctt tcgcggtatc gagagcattc
     7741 gcgccggttg ccagcttaat ccccaggccc gccgccggtt ttccgttgat acgagcgata
     7801 acgttatagt tttcaccgcc aagttcaacc cgtgcgacat ctttcaggcg taccaccgag
     7861 ccgtcactgt ttacgcgcag ggtcactttg ccgaattctt ccggattttt taaccgcgtt
     7921 tgagcaataa tcgaggcatt caactgttgc cctggtaacg ctggcgttcc gcccaactgt
     7981 ccggcagcga tctgatcgtt ctgtaccttc aactggttaa tcacatcaac cggtgtcagt
     8041 ttatatttgt ttagcagatc ggcgtccagc cagatacgca tcgcatactg tgcgccgaaa
     8101 agctgtacgt caccgacgcc attcagacgg ctaagcgtat ctttaacgtt agaggccaca
     8161 tagtccgaga tatcgtcctg tgtggtgtct gggttatcag agacaaagcc cgccaccatc
     8221 aaatagctgc tactggactt ttcaacactg atcccctgct gctgagcctc ctgcggcagc
     8281 aacggcgtgg cgagctggag tttgttctgc acctgcactt gcgcgatatc aggatcggtc
     8341 ccggactgga aggtaagggt aattgtcacg ctaccggcgg aatcgctggt ggaggacata
     8401 tacatcaggt tatcgatacc attcatattc tgttcgataa cctgcgtcac cgtatcctgc
     8461 acggtctgcg catcagcacc tggatagttt gccgaaacag aaaccgcagg cggtgcaatg
     8521 gttggatact gagcgacggg caattgtagg atcgccagtg cgcccgccat catcagaata
     8581 atggccagca cccatgcaaa tatcggtcgt cgaataaaaa agtttgccat gtcagattac
     8641 cttacttcga tgcagtatct gcgggggtat cggtagtggc tttaacctgc tctcccggac
     8701 gcgctttttg caggccgctg acaatgactt gatcgccaga tttcagacct tcgctaatca
     8761 accatttatc gccaatcgcc tgactggcaa cgacagggcg cgcttccacc tgacttttat
     8821 cgttaacaat cagcacggtt gcatcaccac gcggtgtgcg gctaacgcct tgttgcggga
     8881 taagaatggc gtcaggttgg acgccttcat caatccgtgc acgcacaaac atacccggca
     8941 aaagcgtatg ttgcgggtta gggaagacag cacgtagggt tatggagccg gtgctttcat
     9001 caacggtcac atcagagaat tgcagcgtac ctttcagggg ataggtttga ccgttttcca
     9061 tgaccaactc tacgttgctg gtggcgtttt ccttatgcaa atttccttgc tctacggatt
     9121 gcttcagcct cataaaatcg ttgctggatt gggtcacatc aacgtagata ggatcgagct
     9181 gctggacagt cgccagttca gtcgtttgcc cattagtgac aagagcgcct tcggtcacgg
     9241 tcgatttgcc gatacgtccg ctaattggcg cagtcacttt ggtataagcg agattgatgc
     9301 gagcgctttc gactgtggct tttgcggcaa tcacggcggc atcggcctgg cgagcatcag
     9361 caatggcctg gtcgtactcc tgctggctga tgtatttcgt acccacgagc ggaacgtaac
     9421 gttttaccgt caaatgcgcg atggcggcgg cggcttcact tttcgccagt tcgcctttcg
     9481 cgctgtcata atttgcctga taggtcgcgg gatcgatctg gtacagggac tggcctgctt
     9541 gtacatcgct gccttcagtg aaattacgat tcagtacgat cccgctaacc tgtgggcgaa
     9601 cttctgctat acggtaagca ttggtgcggc ctggtaattc agtcttaact tctaacgggg
     9661 ccgttttcac aatatgaacg gtaacctgcg gttcaccgac gtgagctttc tcttctccct
     9721 tatcgttaca accggcgatt aaagccgcgg agatcagaat aaaggagggc aggaggaaaa
     9781 acctggcatg tttcgtcatt actattcctc aaaaaaccaa aagcgcgtta tttacccaga
     9841 aaggcaacgc gttaactcgc agaaagaaaa atacagtttg ctatcctaca aattatcatt
     9901 cgtcgatgta aggaatagtt atgaatactg gcgtctcaag gcacataaac acaaaaaaag
     9961 attaatattc tactgtttta ttttgacgcg ggttgaaaga
//
LOCUS       DAJOSY010000141         9967 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJOSY010000141 DAJOSY010000000
VERSION     DAJOSY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.141"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            322..945
                     /gene="rcsA"
                     /locus_tag="OHN27_003871"
     CDS             322..945
                     /gene="rcsA"
                     /locus_tag="OHN27_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCQ2288234.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWLAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(989..1177)
                     /gene="dsrB"
                     /locus_tag="OHN27_003872"
     CDS             complement(989..1177)
                     /gene="dsrB"
                     /locus_tag="OHN27_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCQ2288235.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            1339..1566
                     /gene="yodD"
                     /locus_tag="OHN27_003873"
     CDS             1339..1566
                     /gene="yodD"
                     /locus_tag="OHN27_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCQ2288236.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQHDPEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            1864..2679
                     /locus_tag="OHN27_003874"
     CDS             1864..2679
                     /locus_tag="OHN27_003874"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCQ2288237.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLRGANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGITHGEISQ
                     VLNTLREKEHFKFTTFDDVDDETIAEWTGLNRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSLGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNRQGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(2676..4370)
                     /gene="yedQ"
                     /locus_tag="OHN27_003875"
     CDS             complement(2676..4370)
                     /gene="yedQ"
                     /locus_tag="OHN27_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCQ2288238.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFISLRNAV
                     TEFEQHRDDHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEVTAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRQ
                     RAVRWFTSQSEHASNTEPQVTVSVPVDSDNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHSTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(4541..4723)
                     /locus_tag="OHN27_003876"
     CDS             complement(4541..4723)
                     /locus_tag="OHN27_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCQ2288239.1"
                     /translation="MSFMISEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            complement(4802..5719)
                     /locus_tag="OHN27_003877"
     CDS             complement(4802..5719)
                     /locus_tag="OHN27_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCQ2288240.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5892..6812
                     /gene="yedA"
                     /locus_tag="OHN27_003878"
     CDS             5892..6812
                     /gene="yedA"
                     /locus_tag="OHN27_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCQ2288241.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGEKLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(6801..7271)
                     /gene="vsr"
                     /locus_tag="OHN27_003879"
     CDS             complement(6801..7271)
                     /gene="vsr"
                     /locus_tag="OHN27_003879"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCQ2288242.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALIERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(7252..8670)
                     /gene="dcm"
                     /locus_tag="OHN27_003880"
     CDS             complement(7252..8670)
                     /gene="dcm"
                     /locus_tag="OHN27_003880"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCQ2288243.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEQLLQIYDVKTLVAHLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPIPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANYYCDPAVHHFNEDIRDITLSHKEGIGDDVAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AIFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISGCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(8737..9432)
                     /locus_tag="OHN27_003881"
     CDS             complement(8737..9432)
                     /locus_tag="OHN27_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCQ2288244.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFAQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTAEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPHTMQTIKGKQLAQYNAQFLVEFMAKLSAELAGENEG
                     IDHKVIDTFSPVG"
     gene            complement(9472..9837)
                     /locus_tag="OHN27_003882"
                     /pseudo
     CDS             complement(9472..9837)
                     /locus_tag="OHN27_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
BASE COUNT         2509 a         2613 c         2352 g         2493 t
ORIGIN      
        1 cagatattat cagcgaatta cctgtaatat gattccctaa tgtttatctt gttatcctaa
       61 ggattatctg aaaaataata cccacgaaca tcttccagga tactcctgtg gcgaaatatt
      121 tgttttaacc tcactcacat atcgcaacat ttactttact ttaagacaat tccaggcaaa
      181 ttatacaaca ctttacggga tagtaagtcc gcctgaaaaa tcgcgagagt ggcgcattag
      241 gtgacccatg ttgttccgtt tagtcatgat gaaatattca ggtaagggga attatcgtta
      301 cgcattgagt gagggtatgc catgtcaacg attattatgg atttatgtag ttacacccga
      361 ctaggtttaa ccgggtatct gttgagcaga ggggttaaaa aaagagaaat caacgacatt
      421 gaaaccgttg atgaccttgc catagcttgt gattcacagc gcccttcagt ggtgtttatt
      481 aatgaggact gtttcatcca cgatgcttct aacagtcagc gtatcaagct catcattaat
      541 caacatccca atacgttatt tatcgttttt atggcaattg ccaatgttca ttttgatgaa
      601 tatctgttgg tcagaaaaaa tttattgatc agttctaaat cgattaaacc ggaatctctc
      661 gacgatatcc ttggcgatat tctgaaaaaa gagacaacga taacctcgtt tttaaatatg
      721 ccgacgttat cattgagccg aaccgaatcg agtatgttgc gaatgtggtt ggcaggtcag
      781 ggaaccattc aaatctccga ccaaatgaat atcaaagcca agaccgtttc atcgcataaa
      841 ggtaatatta aacgtaagat caaaacgcat aataaacagg ttatctacca tgtcgtccga
      901 ctgacggata atgtgactaa tggtattttt gtcaacatgc gctaacacat tctgactggt
      961 ggtttcccac cagtcaggct gaattagatt actctgcttt ctccacaaag ataccgtcct
     1021 gatgccctgc ttcattaaag aaccagatgc cgagcgggta gtcttccagc gaaaccaggt
     1081 acattgtgcc ttcactaaac tcctcaactg ccagtaccac gccaggacga cgcggaccgc
     1141 catccgtttt gactgttacc cgatcattca ccttcatcat tttcctcctg tggctttgtg
     1201 ccagtgtaga acaatttcgt ttttctggca gcgccgggcg cgcgcgagtg ctgattttct
     1261 cgacggtcta tacttaagag atgccagcgg acttaacgac tggcggcaac aacagagtaa
     1321 cggttgcgag gaaagatgat gaaaaccgca aaagagtaca gcgataccgc aaaacgtgag
     1381 gtcagtgtcg atgtcgatgc cctgctggcg gcgatcaatg aaattagcga aagcgaagtt
     1441 catcgcagcc agcacgatcc tgaacacgtt agtgtcgatg gacgtgaata tcatacatgg
     1501 cgtgaattgg cggatgcctt cgaactggat attcatgact tcagcgtctc tgaagtgaat
     1561 cgttgaatgc acaataaaaa aatcccgacc ctgagggggt cgggattaaa cttgcttaag
     1621 caagaagcac ttaaaaaatt cgttacacca ggaaatctga tgtgttcatc accttatccg
     1681 caattttttt cgctgacaag aaaatattcg tgatatgaat gattaacgct acttatgaat
     1741 agaaatgtga cgcgcgtcac cccttggtgg ctctggcgtc aacgggatcg ctcccgaaaa
     1801 aatcatcttt gataatttgt gctgttttga gaatcatcct ggtaagggga gtactgcgca
     1861 accatgcttt caattcaaca accactactg gtttttagcg atcttgatgg caccctgctg
     1921 gacagtcata gttatgactg gcaaccagct gccccctggc tcagccgttt acgcggagcg
     1981 aatgttcccg tcattctctg tagcagtaag acatcagcgg aaatgctgta cttgcaaaaa
     2041 acgttggggc tacaaggttt accgctgatt gccgaaaatg gcgcagtgat ccagctagct
     2101 gagcaatggc aggatataga cggttttcca cgcatcatct caggtattac ccatggcgaa
     2161 atcagccagg tattaaatac gctgcgtgag aaagagcatt ttaaattcac aacttttgat
     2221 gacgtcgacg atgaaaccat cgccgaatgg acgggattaa accgtagcca ggcggcactg
     2281 acgcagttac atgaggcatc tgtaacgctt atctggcgcg acagtgacga gcggatggcg
     2341 caatttaccg cccgtctgaa cgagctgggc ttgcagttta tgcagggggc gcgcttctgg
     2401 cacgtactgg atgcctctgc cggaaaagat caggccgcaa actggatcat cgcgacctat
     2461 caacaatcgt taggcaaacg accaaccacg cttggtctgg gcgatgggcc aaacgatgcg
     2521 cccttactgg aggtaatgga ttacgcggtg attgtgaaag ggctaaatcg tcaaggggtg
     2581 catctgcatg atgaggatcc ggcccgcgtc tggcgaacgc agcgtgaagg accggaaggc
     2641 tggcgtgaag ggctggacca ttttttctcc gcccgttaag cgttatcgct cgcgaatacc
     2701 cgattgcgcc cggcctgttt agcgagataa agtcgacggt cggccagcga ctggagttgt
     2761 tcaaaatcat aatcaccagt ttcctcgctg ctgcttaccc ccagcgaggc actgatgcgt
     2821 atcgtcgtac tcttggcgat caacatctct ttttcattta gcttcaggcg aatacgttct
     2881 gcaacctcag cagcctgcgt caggctcgcg cctggcagaa tcacacaaaa ctcctcgcca
     2941 ccgacccgcc cggcaacgtc ctgcgcacgc aaggaactgc taattaatcc ggcagcatga
     3001 gaaagcacac ggtcgcccgc ctgatgacca aagcggtcat taatcgcttt aaaatggtca
     3061 aggtcgacct gaatgacaga aaaaggatgt tggtgcgtct gacacaattt agcgagcgga
     3121 cgggcttttt cgaacagtgc gccacggtta tataaacgcg ttaaggtgtc gtgccacgcc
     3181 tgccactgca acgagctttg caaaacatac atgttgctga ccatccggcg aatcacatac
     3241 caggagatga gtaacatggt ggtaaagagc gcccacagca gggttaatgc aatgctgata
     3301 ctgccgaaat caccacgcac gccttcgctt agcgtatgga cgcgcaccag cacaccgtcg
     3361 aaatggtcca gacgttccca gctaacatag cgactgttca tacgaatgcc gccccgcgtg
     3421 tcatgttcca ttgcctgcgc cagcaaggcc agttcacgag gatcaaaaat attccctgtt
     3481 gaatggtcag gattagaaga ggtcaataat ctcagcttac tgtcatagag ctgatactca
     3541 ccatcgaggt ttttatcgat ggcgtttctt aaaaattgct gcatggtacg cacgggaata
     3601 ctcatcccca gcacgccata ccagtagtta tcactgtcta ccggaacact gacggtaacc
     3661 tgaggttcag tattgctggc atgttctgac tgcgaggtga accaacgtac cgcgcgttga
     3721 cgattttctc gttgcgaatg accgataaac cagggttggg tgacatagcc gtaataacgc
     3781 gttggtacat tgcgcgtaaa caaggtcggc tgcgtcgaaa cgtaaaatcc ggcacgcgag
     3841 acatacatag cttgttcgac catcgacgag gaattgtgcg ccagtcgtag caagtaacca
     3901 acctccaacg cagcagtaac ttcattgtcg aggctttcat tttcgcgaga caggagattt
     3961 ccctcgctga ctaatgcatc cgaaacgcca ttgactggca gggtgcgtcg tcggttgagt
     4021 tcgatttgcc aggcgtgatc gtcgcgatgc tgttcgaact ccgttacggc attacgcaaa
     4081 gagataaaat ccagtggcgc tacgagagct tcgcgcatgc cattacgtaa aaagatcagt
     4141 ttgtcgacgt tatactgcag atgcttatcg agcgcgttag ctacattttc cagatgatta
     4201 cgctggctgg agatataggc atcttccaga accaccactt cacgccaggt aagcaaggtg
     4261 gaaaaaagca gcactacgat aaagcagaga ttaacgacat gaccaggccc caggcggcgc
     4321 gcaagttttt tcaaccagct ctggttttcc atttttgtct cgtgctgcac cctgacgact
     4381 cccaacctgc ttttttatga ttctggcata acctggcgat agcgtaccgc aaaacagtca
     4441 taattttata atggtggcat gacaagacgc gacagtcatt cattgcaggc attggcacac
     4501 caggccggat gtgatccggc ctggtgttaa tgatactctt tcaaacttct tccgcaggac
     4561 gactcccctc ccccggaaca agctcggttt catgaaaagc ttcccgcttg accccataac
     4621 cgtcatacca acgacactca accataccgc tggaatagcc ggtgacaatc atccgcgggc
     4681 cgccctcttt aaccgttact tcctcactaa tcataaagct catactcgcc tccttttttc
     4741 gagtgaaact tgttcacctt agttgaagat ggcgaatttt gcataatagc cagtgcgagt
     4801 attaatgtgc ctgaccgcgc attttcgcta ccgctttcac tccaagtacc acaatgccgc
     4861 cgatgataaa accaagtatc agatttaaaa cagtcggtaa tatcatcgcc accactgcac
     4921 tttgctgccc ggcgaaatgt tcaatggcgt gatgcagcgg cgcaatacca tgaaccacaa
     4981 tcccgccacc gacgaggaac atcgccaacg tgccgacaat cgataacgct ttcatcagcc
     5041 agggcgcgat aatcaataac cctttaccca atgcctgcat cagcgcgctg gatttttccg
     5101 ccagccaata ccccaggtca tcaatcttaa caataacccc taccagcccg tagacgccca
     5161 cggtcactac cagtgcgatg cctgaaagca ccagcacctg attaagcaac ggcgcttctg
     5221 ccacaatccc cagcgtgatg gcgacgattt ccgcagacaa aataaaatca gtacgaatcg
     5281 ccccttttat tttgtccttt tcaaacttca gcggatcctg cgccgccagc ttctccagac
     5341 gctgctgact ctgcgccgga tcttctttgt gtttacgcgc ctccagcata tgcagcactt
     5401 tctctacccc ttcaaagcag agaaacgcgc cgccaatcat caacaaaggc gtaatcgccc
     5461 acgggataaa tgcactgatg atcagcgcca gcggtaccag aatcacttta ttgatcagcg
     5521 atcctttcgc cactccccag accacgggaa gttcccggtt ggcacgcaca cctgaaactt
     5581 gttgtgcatt gagcgataaa tcatccccta gtacaccggc ggttttcttc gccgccagtt
     5641 tgcccatcac ggagatatcg tccagcagtg tggcgatatc atcgagcaac gtcagtaaac
     5701 tactcccggc caaaagcatt ccctctcatt tttattgttg aagggagtaa gtatgaagca
     5761 aaaacggcag ctctgatact tgcttcgtag gtcaacattt ttttaacaat caagtgcaat
     5821 tatatctctc gccagcagaa tgattttcac gtttactatg aaccaccttt ttattttcac
     5881 cgtgagggat tatgcgtttc cgccagttgt taccgctttt tggcgcgctg tttgcgttgt
     5941 atatcatttg gggctcaacc tattttgtca ttcggattgg cgtggaaagc tggcctccgt
     6001 taatgatggc gggcgttcga ttcctggcag ccggtatttt attgatggca tttttgctac
     6061 tgcgcggaca caaactcccc ccgctacgtc cgctgctcaa tgccgcgctg attggcctgt
     6121 tattgctggc tgtcggtaat ggcatggtga cagttgccga acatcaaaat gttccatccg
     6181 gcatcgccgc cgtagtggtt gcaaccgtgc ccctctttac cctgtgcttc agccgcctgt
     6241 ttggcattaa aacgcgcaaa ctggaatggg tgggtatcgc cattgggctt gccggaatca
     6301 tcatgctcaa tagcggtgga aatttaagcg gcaatccgtg gggtgcgatt ctgattttaa
     6361 tcggctcgat tagctgggcg tttggctcgg tttatggctc gcgcattacc ttacctgtag
     6421 ggatgatggc gggtgcgatt gagatgcttg cggctggcgt ggtgttaatg attgcgtcga
     6481 tgattgcggg tgaaaaactg acggcgctcc cttccctttc aggcttcctt gcggtcggct
     6541 atctggcgct gtttggttcg attatcgcca tcaacgctta tatgtattta atccgtaatg
     6601 tcagtccagc tctcgccacc agctacgcct acgttaaccc ggtggtcgcg gtcttgctgg
     6661 gtacaggact gggcggcgaa aagctgtcga agattgaatg gctggcgctc ggcgtaattg
     6721 tattcgcggt ggtactggtc acgttgggaa aatatctctt tccggcaaaa cccgtagttg
     6781 cgccagttat tcaggacgca tcaagcgagt aaatgaatcc cctgcgtgtc gatctgcgcg
     6841 ctggcacctt cgccgcagat ccactcttcc agacgctcga taagcgcttc atccgtcagc
     6901 ttctcgcgcc cacgtaacgc acactcccag acaatcagta cgcgccagcc gagctcctgc
     6961 aagcgactga tatcgcggcg atcgcgctca acatttttac ctatcttctc cagccagaac
     7021 tcagttcgag tcgcaggcac tttaaacaga tagcagtgat gatggtgcca gaagcagccg
     7081 tgggtaaata tcacgcagcg atattcatca acgacaaaat ccggacgtcc gggcagactg
     7141 gcgtcctgaa cgcgaaatgc caggccctgc ccggttaaca gactggcgag gcgcttctct
     7201 atcgccgtat cacgcgtggc aatcgcgcgc atatttttgc tgcgagtggc cttatcgtga
     7261 acgtcggcca tgttgtgcct cttgctgacg caacgccacc gcctgtttga tttttggctc
     7321 aagcagtttt gccaccgcgg caaagaccgg cacgaccacc gagttaccga actggcgata
     7381 ggcctgagtg tccgaaaccg gaatgcggaa tttcgcttct cccggcgctt caaaacccat
     7441 taagcgcgcg cattcccgag gcgttaaccg acgtggacga tgttgctgat tcagcggatc
     7501 gtcaaagtct ttttcacccg tggccatatc ccatccgcga tcgattaaaa tttccgcgcc
     7561 atctttgtaa taacgcgcag acagcgtacg ggtgacgctt tgcggattgt tcggataaac
     7621 cattccataa ccgaagccgt taccgcgcgc ctgatgtttt ttcgcatatc gatagaggta
     7681 cttccacagt accggcgtca ggatatattt cgcctcgacc atcgggtcca gcagctgcgc
     7741 cagcgtcact cgctgcgcag ggaaacatcc gctgatatca cgcagagtaa aatcggcttt
     7801 cagattcagg tcgcgacgaa aacccaccag cacgatgcgt tcacggtgct gcggaaggaa
     7861 atgtttaccg tcaatgattt tcggatcgtc cgggccatta tcttctgcat ctgccacgtc
     7921 atagcccagt tcatccaacg tctgcatgat gatacggaac gttttcccct ggtcgtggct
     7981 tttcaggttt ttgacgtttt cgagcacaaa aatcgccggg cgacgcgcgt cgataatacg
     8041 caccacatcg aagaacagcg tgccctgggt atcacaggca aaaccgtgcg ctcgtccgag
     8101 tgagttcttt ttcgatacgc cagccagaga aaacggctga caaggaaaac cggccagtaa
     8161 aacatcgtgc tcaggaatat gttgacggat atgttccgcc gccacgtcat cacctatacc
     8221 ctctttgtga ctgagagtga tgtcgcgaat atcttcatta aagtgatgca ccgccgggtc
     8281 gcaataatag ttggctttat aagtgcgtac agcatgtttg ttccattcgc tggtaaacac
     8341 gcactgccca ccaatcgatt caaaaccgcg acgaatacca ccaatcccgg caaatagatc
     8401 gataaagcga aacgcataat gcggatgatg tgccggtgga atgggtaaca acgtttgcag
     8461 atgggcgaac tctttctcac ttaaacggtg ccatgctgag tcattcgcca gcgcacgttt
     8521 taaaattgcc gcgctccagt gattctcacc cacaccatta agatgcgcca ccagcgtttt
     8581 gacatcataa atttgcagca gttgctccag cattgcctgt gcggcattcc cggtgctgta
     8641 tgaatcggtt actgatatat tttcctgcat agatttaacc ggccatctga gatggccgac
     8701 aggttaacat aattaccatc atttagggcc acgtgctcag ccaaccggtg aaaacgtatc
     8761 aatcaccttg tgatcgatac cttcattctc ccccgccagc tcagcactga gcttcgccat
     8821 aaactcaact aaaaattgcg cgttgtactg cgccagttgt ttgcctttta tggtttgcat
     8881 ggtgtgcggt agcttcagca acttagtctg aaaatgatcc agcgcgtaac gtttatcatc
     8941 aagtgggcga tgctgcgcga acggatcttc gccatcaaac aatgccacgc ccaacgcccc
     9001 cgaaacggca aacacacgcg ccaggccaat cgcccccaga gcttccagcc gatccgcatc
     9061 ctgaacgatt ttagcttccg ccgttaaagg ggcgatttgc gcgctaaaac tgtgtgcggc
     9121 aatggcatgg caaacagcct cgattttttc cgctggaaat tgcgcgaatt cttcacgcag
     9181 caggcgacgc gtctcttctg ctgccagtat tgaagaacgc tgccgttgcg gatgattttt
     9241 cgccaggctc acgatatcgt gaaaataaca ggcggttaaa atcaccagca tgtcaacgtc
     9301 gtcatctgcc gccagtttct gtgccgtagc ccagacacgg cgaaaatgac acaggtcatg
     9361 tgccgcgtcc tgatgttgat gatggttttt caaccagttt tcgaactgtg cctgccagtg
     9421 ttgtaagtcc atacgcgctc cgttgccaaa gagtcgcaac cttagcagtt tttattcata
     9481 gcgtctgcta cgtgcgggac gaggtcgggt gtaccaggca atgccgccta gcaaggcacc
     9541 aaccagggcc ataaacagaa atccgaccaa tgctcctaac cacttccccg cagtagatcc
     9601 taaatcgctt tcaaatcccg acacgggggc atcggggatc cggctcatca cccagatgta
     9661 acgcgccatt gcccagacga aatagccaca ccatgtatag aaagctcaca aagccagttt
     9721 gccaccaggg ctgcgagaga gcctctcttc catctttgtc gaaccgtatt ccattttgag
     9781 ataacccgga aaattcagac ttagtactat tatgtgataa aagtcacatt ttaccacatt
     9841 gaatgagcca gtcaagactc aatcattttt tgaacaaata gataaatttt gcgcaacggc
     9901 agtgaataca ggaaaaatcg ttttgagcga ttttatttat ctttgatatc ggctttatta
     9961 attcatt
//
LOCUS       DAJOSY010000142         9625 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJOSY010000142 DAJOSY010000000
VERSION     DAJOSY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.142"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            142..1206
                     /gene="ulaG"
                     /locus_tag="OHN27_003883"
     CDS             142..1206
                     /gene="ulaG"
                     /locus_tag="OHN27_003883"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCQ2288245.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQVDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1314..2069
                     /gene="ulaR"
                     /locus_tag="OHN27_003884"
     CDS             1314..2069
                     /gene="ulaR"
                     /locus_tag="OHN27_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCQ2288246.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2066..2815)
                     /gene="yjfP"
                     /locus_tag="OHN27_003885"
     CDS             complement(2066..2815)
                     /gene="yjfP"
                     /locus_tag="OHN27_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCQ2288247.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2997..3326
                     /gene="bsmA"
                     /locus_tag="OHN27_003886"
     CDS             2997..3326
                     /gene="bsmA"
                     /locus_tag="OHN27_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCQ2288248.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIKAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3475..3750
                     /gene="yjfN"
                     /locus_tag="OHN27_003887"
     CDS             3475..3750
                     /gene="yjfN"
                     /locus_tag="OHN27_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCQ2288249.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3867..5492)
                     /locus_tag="OHN27_003888"
     CDS             complement(3867..5492)
                     /locus_tag="OHN27_003888"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCQ2288250.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5576..6739)
                     /locus_tag="OHN27_003889"
     CDS             complement(5576..6739)
                     /locus_tag="OHN27_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCQ2288251.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPLYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6742..7380)
                     /locus_tag="OHN27_003890"
     CDS             complement(6742..7380)
                     /locus_tag="OHN27_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCQ2288252.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRSTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7390..7788)
                     /locus_tag="OHN27_003891"
     CDS             complement(7390..7788)
                     /locus_tag="OHN27_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCQ2288253.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7806..8465)
                     /locus_tag="OHN27_003892"
     CDS             complement(7806..8465)
                     /locus_tag="OHN27_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCQ2288254.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8516..9214)
                     /locus_tag="OHN27_003893"
     CDS             complement(8516..9214)
                     /locus_tag="OHN27_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCQ2288255.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9233..9619)
                     /locus_tag="OHN27_003894"
     CDS             complement(9233..9619)
                     /locus_tag="OHN27_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCQ2288256.1"
                     /translation="MALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINILFTSRQ
                     MIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSL
                     EDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         2508 a         2534 c         2280 g         2303 t
ORIGIN      
        1 ctttccacac tcaaaatcaa caacaacttc cctggcaaaa agtcaaaaaa tggtcattgc
       61 cttcctgaaa aaatagtgtc aggattaatc aaaaccaatc acatattgat tcgaattgaa
      121 ctatcatgaa ggtaaatggc gatgagtaaa gtgaaaagta tcacccgtga atcctggatc
      181 ctgagcactt tcccggagtg gggtagctgg ttgaatgaag aaattgaaca agaacaggtc
      241 gctcctggca catttgcgat gtggtggctt ggctgcaccg ggatctggtt aaaatcggaa
      301 ggtggcacca acgtttgcgt tgatttctgg tgcggcactg gcaaacaaag tcacggtaac
      361 ccgttaatga aacagggtca ccaaatgcag cgcatggctg gcgtgaaaaa actgcagcca
      421 aacctgcgca ccaccccgtt tgttcttgat ccgtttgcga ttcgccaggt cgacgcggta
      481 ctggcgactc acgatcacaa cgatcatatc gacgtcaacg tcgctgctgc tgtgatgcag
      541 aactgtgctg atgacgtacc gttcattgga ccgaaaacct gtgtggattt gtggattggc
      601 tggggcgtac cgaaagagcg ttgcatcgtg gtcaaaccgg gcgatgttgt aaaagtgaaa
      661 gacattgaaa ttcatgcgct tgacgctttc gaccgtactg cactgatcac cctgcctgcc
      721 gatcaaaaag cggctggcgt actgccggac ggcatggacg atcgcgcggt gaactatctg
      781 ttcaaaacgc ctggcggctc cctgtatcac agcggcgact cccactactc taactattat
      841 gcgaagcacg gtaacgaaca tcagatcgac gtggctttag gttcatacgg cgaaaacccg
      901 cgcggtatca ccgacaaaat gaccagcgcc gatatgctgc gtatgggtga agccctgaat
      961 gcgaaagtgg ttatcccgtt ccaccacgat atctggtcaa acttccaggc cgatccgcaa
     1021 gagatccgcg tgctgtggga gatgaaaaaa gatcgcctga agtatggctt caagccgttt
     1081 atctggcagg ttggcggcaa atttacctgg ccgttggata aagacaactt cgagtaccac
     1141 tatccgcgtg gtttcgatga ttgcttcact attgaaccgg atctgccgtt caagtcattc
     1201 ctgtaatctg tcgtttatac cggatagcgt ggagtagttc gcgctatccg gcgtagtatt
     1261 tcctggctat ttcaaatatc atctaaaaaa atcaaatttt atcggaatag ctcatgactg
     1321 aagcacaaag acatcaaatc ctcctggaaa tgctcgcaca attgggcttt gtgaccgttg
     1381 agaaagtcgt tgagcgtctg ggaatttcgc ctgccactgc gcgacgcgat atcaataaac
     1441 ttgacgaaag cggcaaactg aaaaaagtgc gcaatggcgc agaagctatt acccaacagc
     1501 gcccgcgctg gacgccgatg aatctgcatc aggcgcagaa tcacgatgaa aaagtacgta
     1561 tcgctaaagc ggcctcgcag ctggttaatc cgggcgaaag cgtagtcatc aactgcggct
     1621 ccaccgcgtt tctgcttggg cgggaaatgt gtggcaagcc agtgcaaatc atcactaatt
     1681 atctaccgct ggcaaattac ctgatcgatc aagaacatga cagcgtgatc attatgggcg
     1741 gacagtacaa caaaagtcag tccatcactt taagcccgca gggcagcgaa aacagtctct
     1801 atgccgggca ctggatgttt accagcggca aaggactgac agcagaaggg ttgtataaaa
     1861 ccgatatgct gacggcaatg gcagagcaga agatgctgag cgtggtaggg aaactggtgg
     1921 tactggttga tagcagtaag attggcgaac gcgcgggaat gctttttagc cgtgccgatc
     1981 aaattgatat gctgatcacc ggcaaaaatg ctaacccgga aatcctgcaa caactggaag
     2041 cgcaaggggt cagcattctg cgtgtttaaa gatgctggcg gaaaaatgtc actgcagcat
     2101 ctaacgcctc aggcgtaatg cggtgacgca cgcctggctg ccatgaacag gttagcagtt
     2161 tatcccgtcc cgtctcgctt aaggcctgct gcaaacgtag tgattcgtcg gcaggcacaa
     2221 cgtcatcgtc gaggccatgc cacagcagta gaggtctgtc actaagttgt tccaggtggt
     2281 ttgtcgcttc ccactctgcc agcggcgcga caatgttatt gaattcattc tgctgtgctg
     2341 tcgtttcagg tatcagcggt ggaaacagtg aacgggcgag tgatgtaaaa tagcccgatc
     2401 ccatcatgct ggcggtacat ctcaccgtgg ggtggcgagc ggtaatcccc agtgccgtca
     2461 tcgcgcccat cgacgcacca ccgactgcca gacggtcatc aagcagccac ttttcttcgg
     2521 ctattgccgc acgtaaagta gtgaattcct gcatactttg tagcaagatt tgccagaatt
     2581 gatttaaccg ccgcgctgcg tcgccactaa aacgactacc gtgatcgggc gcatccggca
     2641 tgatcacccg caaaccagcc tgcgccagcg caacggcaaa atagctatac accagactgg
     2701 acgaagtaaa gccgtgataa aaaatcgcgc acggtaacgg ggtatctttt tgtccgacag
     2761 gataagcatg aagaacggga atatctgcca gctcgcgtga ttctatttca atcatctgtc
     2821 tctccttttt gtctggtatg acatgaagaa atccttagca caagtttctg ctggcggtat
     2881 tagttcataa tgttgagatg tgggttacgc tttcgtcacg ttttcattcg aaaatcgcgt
     2941 accaggtaac aattcgggaa cattccccca aagttaaatt aataaggcac tacactatgg
     3001 ttagcaggaa acgtaatagc gttatttacc ggtttgccag tttattattg gtgttgatgt
     3061 taagtgcctg tagcgcactg caaggtacgc cacagccagc accgccagta acggaccatc
     3121 cgcaagagat tcgccgcgac cagacgcaag ggttacagcg aataggtagc gtaagcacga
     3181 tggttcgggg ttctccggat gacgcattag cagaaatcaa agcgaaagct gtcgctgcaa
     3241 aagctgatta ttacgttgtc gtcatggtgg acgaaaccat cgtgacagga cagtggtatt
     3301 cacaagccat tttgtaccgt aaataaatca gacaacttac atagtcagct ttacattgct
     3361 ttgcgtccct atgcgtttac ctgtgcacaa taaattaccg gctggaagcc tgggcgttta
     3421 ttaccgcgat ggaatgccct gcaacatgtg gggaaacgaa aaatggagct gacgatgaaa
     3481 caattacttg cctcaccctc gctgcaatta gtgacttatc ctgcgagcgc cacggcacag
     3541 tctgcagaat tcgccagtgc tgactgcgtg acgggcctga atgaaatagg tcagatctca
     3601 gttagcaata tttcagggga tccgcaggac gtggaacgta tcgtggcgtt aaaggccgat
     3661 gaacaaggtg cgtcatggta ccgcattatt accatgtacg aagaccagca gcctgataac
     3721 tggcgcgtac aggcgatact gtatgcatga gtgtcatcta cagcaaatga acaatgccgg
     3781 atgcggcgcg agcgccttgt ccggcctaca attttcacat atttcattta gttaatcgaa
     3841 accagcgtcg catcagtcgt atacgcttac acacacactc cccccgtcgc ccgcagcaat
     3901 aaatcattct ggatctgctc tgacaaccgt acgccgccgc gcgtatctaa catcacctga
     3961 caccacgcct gcgccattgg cggagaagca tatttcaaca tttgcgcacc gcagcccagc
     4021 aggaatagct gatgagtaat ctctcgcccc agttcttcag ctggcttacg cagctgctgc
     4081 tgtaaacgac gaaccgcgcg atcaaaatag cgatcctgcc ctttcacttc cacaaatgct
     4141 tccgacaata agtcgtatac gcccgcttgc ttattgagaa cgcgcaacac atccaggcac
     4201 ataatattgc cggaaccttc ccaaatactg tttaccggca tctcccggta aagccgcggc
     4261 agctcgctct cctcgcaata accaatgccg cccagcacct ccatcgcttc ggccacaaac
     4321 ggcatacctc ttttgcagat cacaaatttt gccgcaggcg taaataaacg cgcccacagg
     4381 gcttctttgg catcggcacg ccggtcccac gctcgcgcaa gacgaaacag caacgccgtt
     4441 tgcccttcaa gctgaagtgc catgcgactt aagacatgac gcataagggg ctgttggatc
     4501 aatggattac caaaaacatg gcgttgatgt gcatgataaa tcgccagcga aaatgcacgg
     4561 cgcatcatgg catggctacc cagggcgcaa tcaaaacgcg tcatcccacc cattttcagg
     4621 atcagacgaa ttccttcccc ttcctgcccc aacaaccaac caatggcatc ctgaaactcc
     4681 acttcgcaac tggcgttaga gcgattaccc agcttatctt tcagccgctc gaggcgaatc
     4741 gcgttgcgtt gcccgtcagg caaaaagcgc ggcacaaaaa agcaggacag accacccgcg
     4801 gtctgcgcca gcaccagatg cgcatcgctt tgcggaaccg agaaaaacca tttatgcccc
     4861 accagccgat aagagccatc ttccagacgc tctgcacggg tggtgttgct cataacatcg
     4921 gaaccgccct gcttttccgt cattcccatg ccaatcaaca aaccgcgttt ttgcccacct
     4981 ggcaataagt gagaatcgta gcgatcgctc aatagcggcg tggtccagtc ctgaaacggc
     5041 gcgggtaaca tctgtaacaa caatggcgtg gcggcaaagg tcatggttat cggacataac
     5101 gaccctgcct caacctgcgc atgtaacata aaacgcgccg cgcgcgccac aaatgcgccg
     5161 gagcgagcgt cttcttccca ggcaagattg tgcacccgat tggtacatag cgcctgcatc
     5221 agcaggtgcc aggcggggtg aaaacgcaca tcgtccagac ggcgtccttg cgcatcgtag
     5281 cgcaataatt caggcggatt cacattcgcc agccgcccca gttcaaggga ttcagccgtt
     5341 cctaactgct gaccaatact ggctagaaaa tcgctatccc agccagcacc ttcacgcgtt
     5401 accgcttcgc agagcgcgcc atcagacagg tataagttgc tgttatttaa tggtataggt
     5461 tgattaaaaa cggtgtgagt ttgccagtgc actgtgtctc cctccatcaa tggcagtcac
     5521 cattagtatg gtcactgcca tggattcatg acagaaagag attgctaaaa cattcttatc
     5581 cagcaatgta atgcggaacg aaacgtgagg tgtctttggt gatcagtgtg ttatcttcac
     5641 ggatccccat tccgcacgct tcatcatcga caatccagct accgatgagt gtgtagctat
     5701 cgccaaaccg cggcagaggt tgaaacgcct ggtagatcat cggttcatcg gcgtaatcac
     5761 catcagcgtg gtcaacgacg ttattctggc cgtcgaaaat ggtgacattt ccgccttcgc
     5821 gcgagtagag cggtttacgc acatagcttt cgccagcggc aatctgcgat ttttcaccct
     5881 caaaccagga tgcaagaagg ttaggatgac cagggaagaa gcgccaaagc agcggcatta
     5941 gccctttatt actcaagata cttttccata acggctccac ccattgctcg cgacgcttgc
     6001 gcagcagcgg accgttatcg tcacgcatca tccactccag cggatacagc ttaaatgcac
     6061 gctggatgac attatcatca agatcggtca gtacgccgcc gacgcccaaa ccgagatctt
     6121 caatgtagat aaaccgcgac tcctgccctg cctgctgggc gcagtcctgc aagtacagca
     6181 cggtactcct gtcttcatcg gtgtcctgac agcagcaaaa ataaaacggt tcccgactgt
     6241 aaagctcgct aaagcgtgaa atcaggcgtt cctgaatagc attgtactga tcggcatcac
     6301 gcggaataat gccgctgcgc cgggcatcct ccagccacag ccactggaaa taagccgact
     6361 cgtacaatga agttggcgta tcggcgttgt actccagcag cttcaccggc gcattgccac
     6421 accaggcaaa atccatccgt ccatacagcg aaggatcgcg ggcgcgccag ctttcagcga
     6481 tcacatccca gtacaacggc gggatcgcca gttgcgtcag gatctcttca tcttttaccg
     6541 cgcgatccac cacctcaagg cacatctgat gcagttccgc agtcggtttt tcgatctgct
     6601 cttcaatctg gcgcagagta aaacggtaag cccgactctc atcccaatag atttcattgt
     6661 cgatgatatg aaagtcgaaa ccgttgtcag cggcgatctg gtccagatcc cgtcgcacag
     6721 gaacgttgtg tctcagcatg attagcctcc ccaatgccca cgggcgctgg aagaacgacc
     6781 atagccgccg cgagaaacgg tagacgcttt tttggtggta aagccgcccg aagagtaaga
     6841 ctcttttttg ccagaaccgg agcgccaaga gtaatcacca gaagtgctgc gccagactgg
     6901 gcgcgaagca aaagaggaac cgccgctgtt ataaacaaac ggctcatcgc gatctttacg
     6961 aataacccgg cttaacaaaa atccagaaac gaccgggatc caactctgtt caacattgtc
     7021 gtagtagcaa ttttcgtact tagactgaca gttctgctga gtcatattct tcggaacatc
     7081 ggcataaaat gccgttttgg cgttgttcca gccacgcgcg caaatgtcgg cattattacc
     7141 gtcatcaata caatcctgca ctgtcgcgta aaacgttccg tcgccgtcgt tatcgacatc
     7201 gctactatcg ctacaacctt tcaatacgaa aaaagcggca ccgcccatta gcgccaacgt
     7261 taagtatttt tgtgcgtagc gattgcgacg cggttcaaaa ggattattcg gtctaccaat
     7321 gcgactaatc gctccgtgac caattttact gttatttctt gatttgcgtt ttctggccat
     7381 gatcgtccat taccatgtca tacaagctgc gttaaaaata ccgccagcca gcgcggcggt
     7441 tcccataaac attcctgctg cggtattgtg attaataatt ttttcgctta atgcgggcat
     7501 ataaagtctg acaccagcaa aaacgagtaa ctgaatcacc agtgcaatcc cgccccaggc
     7561 gaaatagtct ggaatactca ccgcattgat tgccgcactg gataagggga taacgtaacc
     7621 caacaatgta ccgctgaacg ccagtgacgc cgcggtatta ttgtttttga ttaactgcca
     7681 ttcgttgtgc ggtgtaattt tagaatagat aaacagaaaa ataatcacca tagccacgcc
     7741 aataaaaaaa taggcgctaa aggcaagaag tgaatccagt atatgcattg taaacgtcct
     7801 tgtaattaac caataatatg aagtgatgtc agcgggatat cgacacccaa cgcgcgcgaa
     7861 aataaccact ctggctcgcc gagatcgtta aaagattcct caccatttag cagcaaatat
     7921 tcgtaagtat cttcggttac ttgccgttgg tagcccatgg taaaattatg gacctcccat
     7981 ttggcatggt tttgattttc tactttttcc agcatgtaga cgggttcgat atttcccggt
     8041 tcttcgctgt taaaaaatcg ctgccagcgt ttttcctgcc agtttaaggt cattgctccc
     8101 atcgctttgg cgttgatcgc ctcgcgccag tgactctctt tactgatacc gtagctctct
     8161 tcatagacaa agagcttgat atcatcaatg tcatcaatat cttcgccgcc ggtggtattg
     8221 atttgcagaa actcatcgcc tgaagtgtag tagcggaaaa tctgactacc gccgcccaga
     8281 tcgatgtggc tgacggcggc taccgtaaat tcctcacccg gcagcgcaat cagcaattca
     8341 tcttccagca aacgaaacgc taacgtgtcg agcgtaaagc cactattgag atgaagtccc
     8401 agcggaccac gagcgatagc tggcttatta tccttgccaa acagacgctg gaaaaaacca
     8461 gacatatttt atccttaaaa tagttgccag ccttttagcg aggctggcaa aaaaattact
     8521 cgccctgttg gcgttgcagt cttgctaata catcctgggc gctactttta ttgctaccgc
     8581 caattccggc ttccgccagc ttttcgtcaa ggtcgcgacc gtctgcgact ttctccaact
     8641 gtgcggcagc atccaggcga gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg
     8701 attctgccgc tgtcgaaacg ctggaagatg cgccaacggt agaggttgtt acagcctgtt
     8761 gtgcacgctg catggcttca gtggctttaa cgacttccat ttgctgctca aactgagcga
     8821 tacgctgcgc tgtcgctgta actgcttttt ccacgccatc acgggagact tccagattcg
     8881 acaacacttg ctcttcagcg gtaatgagat tctcaaggcg cgcgatttct tcagcaactt
     8941 cgttaatcaa cgacggatta acgttcttgc ttaacgcttc cagcgcacgg gcttccagac
     9001 tggctttgcg ctcacgtaaa tctttcagct tatcgtgact taatttcacc cgcgccagca
     9061 gatcaacgcg agattttccg gctttatcga gttcagcttt agcgtcacga atatgctgtt
     9121 ccagcatgcg cacgccctgg gtttcttcaa tggattcttc cgcctgggag ataaacgatt
     9181 tccccagcgt aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagtgtg
     9241 aatattcttc tgtaatttca gccagatcca atgcgttatc taccagcgag gttatctcaa
     9301 gcaggatatc ttcaagagaa gatttaagcg ataacgcccc gaaaacaatg taatactctt
     9361 cctgttgtac gctggagatc cctaccgatg acaacggcat catcttctga tttcttaata
     9421 agaaggtatt aaattcatct ggattgctga tactactcac cggacaaata aaggtttcga
     9481 taatcatttg gcgggaagta aaaagaatat tgatttgcaa atcgccataa tcattcattt
     9541 taataattaa tgcgttctcg ctatttgtta catcaatatt ttgttcaggt acagtttgca
     9601 gcgccgtcgc tagcgccaac ggatt
//
LOCUS       DAJOSY010000143         9554 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJOSY010000143 DAJOSY010000000
VERSION     DAJOSY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.143"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(354..770)
                     /locus_tag="OHN27_003895"
     CDS             complement(354..770)
                     /locus_tag="OHN27_003895"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCQ2288257.1"
                     /translation="MYDALFCARLFRTFNVVDDFNYEALSIENNLNLPASQVVAVLCR
                     IAENHVYPAMIRMDYGQEFFDPTRTECAEKHVVKLEITQSGKLAQNAFIESFNRTYRT
                     KILGFCLFRTLDEKRELAANWLSEYNSERHINHLTI"
     gene            2485..3672
                     /locus_tag="OHN27_003896"
     CDS             2485..3672
                     /locus_tag="OHN27_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCQ2288258.1"
                     /translation="MLMKYISHRAIICTLTLLIIIITVLFTFLRSSDVPEYITAPVRK
                     GDIENSVLATGRIDAIERVNVGAQVSGQLKSLKVKQGDHVTKGQLIAEIDDLPQRNDL
                     RNAEAALNEVKAELQSKQALLKQAELRFKRQLRMLRENASSHEDFESAEAMLATTRAE
                     LLSLNAKLVQAQIEVDKKKLALEYTRVVAPMDGIVIAIVTQQGQTVNSNQSAPTIIKL
                     ARLDVMTIKAQISEADITRISVGQKARFSIFSEPDKHYSATLRAVELAPESVMKDDSL
                     ASNSSASGSGTSNASVYYNALFDVPNPENRLRIAMTAQVTLITDEAQNTLLVPIQAVH
                     RNEGKKQQVLVLAADGRLEPRDVKTGITNSVDIQILEGLNVGENVVLSLPDKKEPEER
                     IML"
     gene            3669..5609
                     /locus_tag="OHN27_003897"
     CDS             3669..5609
                     /locus_tag="OHN27_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HCQ2288259.1"
                     /translation="MKKLIELKGVSRTYGNGDQTRTVLKNVDLTIVAGEMVAIIGASG
                     SGKSTLMNIMGCLDVPNRGDYYIDGQNAACLSPDELARVRREHIGFIFQRYHLIPDLS
                     ALGNVEIPAIYANSERDSRRQRATALLGRLGLEGREHHKPCELSGGQQQRVSIARALI
                     NGGKIILADEPTGALDSQSGQEVLAILNELNRRGHTVVMVTHDMKVARHAKRIIELCD
                     GEIIADSGGCVSATETLPQTNSIRQSYWKTLLDRTRESMQMALKAMKTHRLRTTLTMI
                     GIVFGIASVVTVVALGEGARQETLEEIKSLGTNVVSIYPGQDLFDDSIESIRTLVPAD
                     ANALAKQGFIDSVSPEVSASDNIRFLGKSAIASINGVGREHFRVKGIELLQGTTFRDD
                     RDALQEVIIDENTRKAIFDNTGLQALGQIVFLGSVPARVVGIAKSNNRSDASNRITVW
                     MPYSTVMYRIVGKPVLTGISVRLKDNVDNEAAISAISQLLTRRHGIKDFQLYNFEQIR
                     KSIEHTSMTFSILILMVACISLMIGSIGVMNIMLISVTERTHEIGVRMAVGARRSDIM
                     QQFIIEAVLVCLIGGALGIALSYITGALFNALADGIFAAIYSWQAAVAAFFCSTLIGI
                     IFGYLPARKAARMDPVISLASE"
     gene            5613..6983
                     /locus_tag="OHN27_003898"
     CDS             5613..6983
                     /locus_tag="OHN27_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HCQ2288260.1"
                     /translation="MKILSSLTLCLITALCSGCTNVLKSDYRAPEVNYPINWTKGDVD
                     GNTSPFDWEEFNDPNLDNWLHLVMTSNNDIAIAALRIHRAQLDAERTGITNTPALKAA
                     LSMDGKKQLNNSSGWAKSGSASLGTSYELDLWGKIARQRDVAEWAVHASEEDFRSARL
                     MLLSEASNNYWRIGFVNQQITTLQQSIDYAKETLRLAEVRYRAGNISSLDVIDAQQNL
                     LTQENQLTGLQREHSQLLNQQAVLLGTVPGCQIVEPTTLPKGSLPKVNANIPASILMR
                     RPDISAKEWQLREALATVDIKRSEYYPTFNLTGALGTSSASLLALLHNPVGSVGANLT
                     LPFLEWRQRDIEVKIARNDYEQRVLEFKQLLYKAMSSIEDALSFRNQLLLQETRLREE
                     LELARKSEWLNEVRYRHGAVRISFWLDAQEKRRQAELRLDENRFNQLQNLAKIYLEFG
                     GASTFP"
     gene            7457..7915
                     /gene="tnpA"
                     /locus_tag="OHN27_003899"
     CDS             7457..7915
                     /gene="tnpA"
                     /locus_tag="OHN27_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HCQ2288261.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGCILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            8025..9338
                     /locus_tag="OHN27_003900"
                     /pseudo
     CDS             8025..9338
                     /locus_tag="OHN27_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         2725 a         2151 c         2323 g         2355 t
ORIGIN      
        1 agtaatcaac agacttgtat gtaaagtcaa gattaacgtt gctaaagcac gcatcaattt
       61 tctgataaaa ggatggggtt gtttttttcg ataaaaaaaa ttcttgtcta accgctggtg
      121 aaataagatt attattaccc cagattaata ctgagcctaa caatagaata caaaaaaaac
      181 aaaatgcttt aaataagggg agcactataa gttatcctct ttactttatg cttaccaact
      241 cattttgtta ctttataaca taactcatct tgcgttgtaa acagactcgt tttagttcca
      301 tgcacctttt gagatcgtga ccaaataatg gcgttagtga tgttcttcca gtgtcatatg
      361 gttaagtggt tgatatggcg ttcactgtta tattctgaca accagtttgc cgccagttcc
      421 cgcttttcat ccagcgttct aaacagacaa aaaccgagta tttttgttcg gtatgtccgg
      481 ttaaaactct cgataaaagc gttctgcgcc aacttacctg attgggtaat ctccagtttt
      541 actacatgtt tttctgcgca ttccgtccgt gttggatcaa aaaattcctg gccataatcc
      601 atgcgaatca tagctggata gacatggttt tccgcaatcc tacagagtac cgctaccact
      661 tgtgaagctg gcagattcag attgttttca atcgacaacg cctcatagtt aaagtcatca
      721 acgacattga acgttcggaa aagacgagca cagaacagag catcatacat aaaatcaaca
      781 gagcaactct gattaactgc ttccggcgta gtgagtgacg aggattacgc accgacaacc
      841 gttgtttgcc cctacggaga aaagcagcct ttatgcccag atagaagaag tacggtcctc
      901 ttccactaat aaagaaccgt cagatgctca ggctcgccat atccagaaag agctgaagca
      961 ggttaccaac gaacgggaca tactaaaaaa gtgcggcgta cttcgcaaaa cggtccgact
     1021 gaggtattta tccatgacaa tatctggtgc tggactgttc tcctgatcta tcggatgctg
     1081 gatattcatc cctgtggctt gactcctggc ttcagttgcc gcattcacaa cgccatcagg
     1141 tagagcttaa acagacagga cagattaaac agttctggtt ggaatcgtgg gcatcaatgg
     1201 ttatcacaat ctccctatgg agctgcggga ttgcgggcaa cagaggagaa ccaacagagt
     1261 ctagcgaccg attaaatatg ccgggataaa agttcagctc ggataccgga atccgcaggc
     1321 acataaaggt caggtcagaa tagtgtcgcc caacaggctc cagctacaat tcaacccagc
     1381 tgctccggat ggggttgctt ggataacaga aaaatctaca tcagtaccca agatggctgt
     1441 atcttgctgt gtttgttgag ctatgcttac ccaaaataat cagctggtca atgcaatgcg
     1501 ctcatgacaa tatcctttgt catgagcgca ttgctgatgg atgtatagcg gcgtaatccc
     1561 caaaaaaaga tgctggttgt cagtaaacaa gccatgaatg acagtcgttc ctgaaatcat
     1621 aagacctgga ggagtgtatg ctccgtcgca gtaattacca cgatacgctt gcagaaattt
     1681 tttccagtta ctgaagcgtg aacggataga gaaaagctct tcggaacgcg ggaagacgcc
     1741 tccaacgata tttttgaaca tcgaaatgtt ttataacaat aagggtcggc atggttcctg
     1801 cgaacagatg tcaacaatag aagataaaaa ccattactct caacaaattg gaagaagtaa
     1861 ggctacccgt actcaaaatt agatcattga tggagatata aatggctcac tttctatcgg
     1921 ttgttgacga tacgtttatc aagtatacat gagaatttct tcataccagc tatatgagac
     1981 gttatgacta ataatcttac tcccaaaatg actatgttgt tgcagtttgg tttattgact
     2041 cttgtctata gttcgccgaa tgccaagata agggagcgag gaactttact gtctacgcct
     2101 ctcttcctag ttacaaagac aagcgtaaaa ctcaccatcc attaaaacta aaggcacttt
     2161 tctgagcaga gttttcatct acaagataaa agctatttta cagccgtaaa gtccagtgaa
     2221 acgaaagaga ggttagtgaa ttaatcactt gatatattaa agtataatgg gcgtagatag
     2281 caccttaata cgaaatcttg tcaggctttc ccggtaatta gtaatactgt tgaaacaaag
     2341 cgagaagggg tcagcccaat ttacgcaaat aaataataat cttgcttatg attaattcca
     2401 aataagtata tttttccagt agatgctgag cattaactaa gagaacccag aacaccactt
     2461 tcattgttat ggaaaacatg gacaatgtta atgaaatata tctctcaccg cgccatcatt
     2521 tgtaccttga cactgttgat cattattatc accgttttgt tcacctttct gagaagctct
     2581 gatgtgccag aatatattac tgccccagtg cgcaaaggcg atatagaaaa ttctgttctg
     2641 gcgactggcc gtattgatgc aattgaacgg gtaaacgtag gggcacaggt atcagggcaa
     2701 ttaaaatcac tgaaagtgaa acagggcgac catgtgacta aagggcagtt gattgccgaa
     2761 attgatgacc tacctcagcg taacgatttg cgtaatgccg aagctgcact caacgaagtg
     2821 aaagcagaac ttcaatctaa gcaggcgctc ctgaaacagg ctgaactgcg ttttaaacgc
     2881 cagctccgga tgttaagaga aaatgccagc tcacacgaag attttgagtc tgcggaggca
     2941 atgcttgcga ccactcgtgc tgaattactg tctctgaatg ccaaactggt ccaggcacag
     3001 atagaagtag ataagaaaaa gcttgccctt gaatatacgc gagtcgtagc gccaatggat
     3061 ggaattgtta ttgccattgt cactcagcaa gggcaaaccg ttaactcaaa ccagagtgcc
     3121 ccaacaatta ttaaactcgc tcgtctggat gtcatgacca ttaaagcaca gatctccgag
     3181 gctgatataa cccgtatttc tgttgggcaa aaagcccgtt tctctatctt ctccgaaccg
     3241 gacaagcact acagcgcaac actgcgtgcc gttgaactgg caccagaatc ggtaatgaaa
     3301 gatgactctc tcgccagcaa cagttcagca tcaggctccg gaacttcaaa tgcgtccgtt
     3361 tattacaatg ctctattcga tgtgcctaac ccagaaaatc gacttcgtat tgccatgact
     3421 gctcaggtta ccctgattac tgatgaagcc caaaatacac tgctggtacc cattcaggca
     3481 gtgcacagaa atgaggggaa gaagcaacag gtcctggtgc tggcagcaga tggcaggctg
     3541 gaaccccggg atgtgaaaac tggtatcacg aacagcgtgg atattcagat cctcgaaggt
     3601 ttaaatgtcg gagaaaatgt cgtgctgtcg ctgccggata agaaagaacc cgaagaaagg
     3661 attatgctgt gaaaaaactt attgagttaa aaggggttag ccgaacttat ggcaacggag
     3721 atcaaacgcg gaccgtcctg aaaaatgtcg atttgacaat cgttgctgga gaaatggtgg
     3781 caatcattgg agcttctggt tcaggcaaat caaccttaat gaacataatg ggttgccttg
     3841 acgtacccaa tagaggtgat tactacatcg acggacaaaa tgccgcctgt ctttcacccg
     3901 atgagctggc cagagtgcgc cgggaacaca ttggctttat attccagcgc taccatctga
     3961 tacctgatct tagcgcactt ggtaacgtgg agatcccggc catttacgcc aacagcgaac
     4021 gcgacagccg tcggcaacgt gcaacggcgt tgctgggcag actgggactg gaaggacgtg
     4081 agcatcataa accctgcgaa ctttcaggcg gtcagcagca acgcgtgagt attgcccgag
     4141 ccctgatcaa cggtggaaag ataattttgg cggacgagcc aactggtgct ctggattcgc
     4201 aatccgggca agaagtgtta gccattctga atgaacttaa tcgacgtggt catactgtag
     4261 taatggtgac ccacgatatg aaggtcgccc ggcacgcaaa gcgaatcatt gagctgtgtg
     4321 acggcgaaat catcgctgat agtggaggtt gcgtatctgc gacggaaaca ctaccacaaa
     4381 ccaacagtat ccggcaaagc tattggaaaa cactgctcga tcgaacgcgt gaatcgatgc
     4441 aaatggcgct aaaagcgatg aagacgcatc gcttacgcac cacgcttacc atgatcggga
     4501 ttgtttttgg tatcgcctcc gttgtcactg ttgtggcatt gggtgaagga gctaggcaag
     4561 aaacactgga agagattaaa agtctgggga caaacgtggt cagcatttat cccgggcagg
     4621 atttgttcga tgacagcatt gagagtatta gaacattggt acctgctgac gcaaatgcac
     4681 tggcgaagca agggtttatc gatagcgtca gcccggaagt tagcgcttca gacaacattc
     4741 gttttctggg taaatctgcg atagcgtcca ttaacggcgt tgggcgggaa catttccggg
     4801 taaaaggtat tgagttgttg caagggacta cttttagaga cgatcgcgac gctctacagg
     4861 aagttattat cgatgagaat actcgtaagg ccattttcga caacacaggg ttacaggctc
     4921 tggggcaaat agtctttctt ggttctgtac cagcacgagt ggttggcatc gctaaaagta
     4981 acaaccgtag tgatgcatca aatcgcatta ctgtgtggat gccttacagc acggtgatgt
     5041 accgtattgt gggtaaacca gttctgaccg gtattagcgt caggctaaaa gacaatgtgg
     5101 ataacgaggc tgcaatcagc gctatctccc aactattaac ccggcgtcac ggcataaaag
     5161 acttccagct ttataacttt gaacaaataa gaaagtctat cgaacacacc tcaatgacct
     5221 tcagtatttt aattcttatg gtcgcatgta tctcactaat gatcggcagt atcggggtga
     5281 tgaacatcat gttgatttct gtaacggaac gaactcatga aattggcgta cgaatggcgg
     5341 ttggcgcgag acgcagcgac attatgcaac agtttattat tgaggccgta ttggtttgcc
     5401 tgattggtgg cgcactcggt attgcactgt cgtatatcac aggtgcgctc tttaacgctc
     5461 tggcggacgg gatattcgca gctatatatt cgtggcaggc tgctgttgct gcattttttt
     5521 gctcaacctt aatcggcata atcttcgggt atctcccagc ccgcaaggca gcaaggatgg
     5581 acccggttat ttcactggcc agcgaataat atatgaaaat tttatcatcg ttgactttat
     5641 gccttatcac cgcactctgt tcaggttgca caaacgtgct gaaaagtgac tatcgcgctc
     5701 ccgaagtgaa ctatcccatc aactggacca agggcgatgt ggacggaaat acatccccgt
     5761 ttgactggga agaatttaac gatcctaatc tcgataactg gcttcacctg gtgatgacaa
     5821 gtaataacga catagctata gctgcactgc ggatacatcg ggcgcagctg gatgcggaaa
     5881 gaacaggtat caccaatact cctgcactaa aggcggcact gagtatggac ggaaaaaaac
     5941 agctgaataa ctcgtccggc tgggcaaaaa gcggttctgc cagccttggc accagctatg
     6001 agctggatct ctggggaaaa attgcccgtc agcgtgatgt tgctgagtgg gcagtccatg
     6061 ccagtgaaga agattttcgc tccgcgcgcc tgatgctgct ttcagaggcc agcaataatt
     6121 actggcgtat cggttttgta aatcaacaaa ttaccaccct ccagcagagt attgactatg
     6181 caaaagagac gctgcgtctg gccgaggttc gctatcgtgc aggaaatatc tcgtcgctgg
     6241 atgtgattga cgcgcagcaa aacctgctca cgcaagagaa ccagcttaca gggctacaac
     6301 gagaacattc acagctactc aatcagcagg ccgttttgct cggcaccgtg cccggttgcc
     6361 agatagtcga gcccacaacg ctgccaaaag gaagcctgcc taaggtcaat gcgaatattc
     6421 ctgccagcat actgatgcgc cggcctgaca taagtgctaa agagtggcag ttgcgtgagg
     6481 cgctagctac ggttgatatc aagcgtagcg aatattaccc caccttcaac ctgacaggcg
     6541 ctttgggtac cagcagcgct tcgctactgg cattactgca taatccggtc ggttccgtag
     6601 gcgctaacct gacgctaccg ttcctggagt ggcgacagcg ggacatagag gtaaaaattg
     6661 cccgtaacga ttacgaacag cgagtgctgg agtttaaaca gctactttat aaggcaatga
     6721 gcagtataga ggacgcgctt tcatttcgta accagttgct gcttcaggag acaagactca
     6781 gggaagaact ggaacttgca cgtaagtcag agtggttaaa cgaagttcgt taccggcatg
     6841 gtgcagtacg tatttcattc tggcttgatg ctcaggaaaa acgtcgtcag gcggagctgc
     6901 gactggatga aaaccgcttc aatcagttgc agaatctagc aaaaatttat cttgagtttg
     6961 gcggggcatc aacctttcct taagtctaat tggggacaac caggagcaat aaggagtgaa
     7021 aaatagccac agctgaaaac accatagcca ttgacatttg agctttacct gctcttgaaa
     7081 acaaaaaatc actgttaagc gcaatgctat tcacttcagc agagcagtat tgcgtgtcac
     7141 gagcatgttt accattctgt gtaaatgtct tttcttaaaa gtgatcatca aagcagtcat
     7201 caaactcgat aaatatccac cggcatagcg taatgccgag cagttaagga tcaattgacc
     7261 gctccttaaa ctgcggcact ataacggctt ctacaacagg gagccgtttt ctatgcacaa
     7321 gaaaaccccc agctaggctg ggggttccgg aaagctttca gctttgagtc agttattaaa
     7381 accccttttg atttgttaaa acatcttgcg gtctggcaac tgcaagtgtc aaacaagaaa
     7441 tcaaaagggg gtcccaatgg ggaacgaaaa gagcttagcg cacacccgat ggaactgtaa
     7501 atatcacata gtatttgcgc caaaataccg aagacaggtg ttctacagag agaagcgtag
     7561 agcaataggc tgtattttga gaaagctgtg tgagtggaaa agtgtacgga ttctggaagc
     7621 tgaatgctgt gcagatcata tccatatgct tgtggagatc ccgcccaaaa tgagcgtatc
     7681 cggctttatg ggatatctga aagggaaaag cagtctgatg ctttacgagc agtttggtga
     7741 tttgaaattc aaatacagga acagggagtt ctggtgcaga ggatattacg tcgatacggt
     7801 gggtaagaac acggcgaaga tacaggatta cataaagcac cagcttgaag aggataaaat
     7861 gggagagcag ttatcgattc cctatccggg cagcccgttt acgggccgta agtaacgaag
     7921 ttggatgcaa atgtcagatc gtgtgcgcct gttagggcgc ggctggtaag agagccttac
     7981 ctactcattt gaaaaacctc cggctatgcc ggaggatatt tattatgcaa tatttcaatg
     8041 atctgctcga tttcagtgac catccgctta tgcctccggc ctctgcacaa ctgtttgcag
     8101 aacacctgcc cgacgagtga atacagcact gcctgacgct ttctgttcat gcggccgttc
     8161 gccgccgtca tttaccgggg agacatagtt atctggatgg tggtggtcat ggccttcttc
     8221 cgcaatgagc caattaccga tgttgttcgc cgtctgaagc tgagcgcgga tggcgaagcg
     8281 gggataaacc tcctgacccg cagcgtggtc acccaggcgc gtcagcgcgt gggggccgcc
     8341 ccggtggaat gactcttcag ccaggctaca cagatatggg gcgcggaacg ttacctgaag
     8401 gataactggc acggtctcca gctttttgct attgatggcg cgcagttaag gacatctgat
     8461 gaacctaggt gcgtgaatat tatggctttg ccaacacatc cactgagcgg cagagcgcct
     8521 acccgattat gcgtctggta acattgatga atctgggaag ccacattttg ctgaatgttg
     8581 tgaccatacc ttaccggcgg agcgaaaccg tgctggctca ctccatgctc gccgccatcc
     8641 cggataactc tattaggctg tttgacaagc acttttacaa tgcagaccag ctgctgacgc
     8701 tgagcctgca gggtggtaac cgccactggc tactacctgc gtggaattat atcgcatcag
     8761 aaacagaaga aagttacggt cccagataca ggcttctgaa actgaagatg tcactgccgg
     8821 ccaggaaagg aattctgcac tgccagagct ctggtacgcc cgagcggtaa cttatgaggt
     8881 gaactgtgtg gagaaaacgg tgctgacatc actcccggca gaccgctaca aagcaaaaga
     8941 ggtggcagaa ctttactatt cacgatggga aatcgaagtc gggtccagaa acctcaaaag
     9001 cagccagctg aacaatgccc tggtgctgag aagccgtagg gttgaggtgc tggagcagga
     9061 gatgtggggc atgttgctgg cttataagct gatacgacgt gaggcaacca aagcggcgtc
     9121 agaaatcagc tttaagttcg cgttccagtt tatcgcgaca gaaatgatag tgctgggaaa
     9181 tacggcatca ccggggacca tcccgaaacg actggtgcat ctgcgggtac tctggaagca
     9241 gtgttcataa cgaaacgccc cgaccatcaa ggccgaaggc agttaagata tcaaaaaaat
     9301 attatccggt gaaacgcagc accgctccgc ttaagtgaac ggcattgtcg gcatagcggg
     9361 agttttttca aatgagtagg taaggctctc ttcccagccg taccctagcg ggcgcatacg
     9421 atctgacatt tgcatctaac ttttaattac gatctgtaaa ctggctaccc agatagagaa
     9481 tcatgaatcg ccacaggttt aacagacacc tccaggacat taaaaatggc tctgaggtgc
     9541 ccataatcag taag
//
LOCUS       DAJOSY010000144         9222 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJOSY010000144 DAJOSY010000000
VERSION     DAJOSY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.144"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..941
                     /locus_tag="OHN27_003901"
     CDS             <1..941
                     /locus_tag="OHN27_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCQ2288262.1"
                     /translation="GIPVHDTIARVVSCISPAKFHECFINLMRDCHSSDDKDVIAIDG
                     KTLRHSSDKSHRRGAIHVISAFSTMHSLVIGQIKTDKKSNEITAIPELINMLDIKGKI
                     ITTDAMGCQKDIAEKIQKQGCDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMS
                     EKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYY
                     ISSVDLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAIN
                     ILTNDKVFKAGLRRKMQKAAMERNYLASVLAGNGLS"
     gene            1212..3459
                     /gene="nrfB"
                     /locus_tag="OHN27_003902"
                     /pseudo
     CDS             1212..3459
                     /gene="nrfB"
                     /locus_tag="OHN27_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            3446..6418
                     /gene="nfrA"
                     /locus_tag="OHN27_003903"
     CDS             3446..6418
                     /gene="nfrA"
                     /locus_tag="OHN27_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCQ2288263.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALINPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6419..7309
                     /locus_tag="OHN27_003904"
     CDS             6419..7309
                     /locus_tag="OHN27_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCQ2288264.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNEQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7492..8253
                     /gene="envY"
                     /locus_tag="OHN27_003905"
     CDS             7492..8253
                     /gene="envY"
                     /locus_tag="OHN27_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCQ2288265.1"
                     /translation="MQLSSSKPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(8299..8375)
                     /locus_tag="OHN27_003906"
     tRNA            complement(8299..8375)
                     /locus_tag="OHN27_003906"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8339..8341),aa:Arg,seq:tct)
     gene            8618..>9222
                     /gene="fimZ"
                     /locus_tag="OHN27_003907"
     CDS             8618..>9222
                     /gene="fimZ"
                     /locus_tag="OHN27_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCQ2288266.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELI"
BASE COUNT         2375 a         2312 c         2323 g         2212 t
ORIGIN      
        1 atggcattcc tgttcacgat accattgcca gagttgtatc ctgtatcagt cctgcaaaat
       61 ttcacgagtg ctttattaac ttgatgcgtg attgtcattc ttcagatgat aaagacgtca
      121 ttgcaattga tggaaaaacg ctccggcact cttctgacaa gagtcaccgc aggggagcga
      181 ttcatgtcat tagtgcgttc tcaacaatgc acagtctggt catcggacag atcaagacgg
      241 ataagaaatc taatgagatt acagctatcc ctgaacttat taacatgctg gatattaaag
      301 gaaaaatcat cacaaccgat gcgatgggtt gccagaaaga tattgcagag aagatacaaa
      361 aacagggatg tgattattta ttcgcggtaa aaggaaacca ggggcggcta aataaagcct
      421 ttgaggaaaa atttccgctg aaagaattaa ataatccaga gcatgacagt tacgcaatga
      481 gtgaaaagag tcacggcaga gaagaaatcc gtcttcatat tgtttgcgat gtccctgatg
      541 aacttattga tttcacgttt gaatggaaag ggctaaagaa attatgcgtg gcagtctcct
      601 ttcggtccat aatagcagaa caaaagaaag agccagaaat gacggtcaga tattatatca
      661 gttctgttga tttaaccgca gagaagttcg ccacagcaat ccgaaaccac tggcacgtgg
      721 agaataagct gcactggcgt ctggacgtgg taatgaatga agacgactgc aaaataagaa
      781 gaggaaatgc agcagaatta ttttcaggga tacggcacat cgctattaat attttgacta
      841 atgataaggt attcaaggca gggttaagac gtaagatgca aaaagcagcc atggagagaa
      901 actatctggc gtcagtcctt gcggggaacg ggctttcgta atcttgccct gcctgctcac
      961 ctcctccagg cacttccact acatgatgac ctgctcggtg ccaccgctcc ttttcactat
     1021 gacgttaacc tccccgtccc aggacaccat gcttttgtct acattctacg caaacggcaa
     1081 cttttataac cagtgtaaaa ataacgtgcc gcaatagtca tacctattaa tggtaaaaag
     1141 ctgtcacaat tcataaaaaa ccttaatata cgccacccta aacataacca gcgttaatgt
     1201 aaggtttttg tgtggactgg cttcttgatg tttttgctac ctggctctac ggcttaaaag
     1261 taatcgcgat aacgttagcg gtcatcatgt tcatcagcgg gctggacgat ttttttattg
     1321 atgtcgtcta ctgggtacgc cgcattaaac gcaagttgag tgtttatcgc cgctacccgc
     1381 gaatgagtta ccgcgaactg tataaaccag atgaaaaacc gttagcgatt atggttccgg
     1441 catggaatga aacgggcgtc atcggcaata tggccgagct ggcggcgacc acgctcgact
     1501 acgaaaacta tcatatcttt gttggcacct accccaacga ccccgatact cagcgtgatg
     1561 ttgacgaagt gtgcgctcgc ttcccgaatg tgcataaggt agtctgcgcg cgtcctggcc
     1621 ccaccagcaa agccgactgt ctgaacaacg tgctggacgc catcacccaa tttgagcgta
     1681 gcgccaattt cgcttttgct ggttttattc tgcatgacgc cgaagatgtg atttcaccga
     1741 tggaattgcg tctgttcaac tatctggtcg agcgtaaaga tctgattcag atcccggtgt
     1801 atccgttcga acgcgaatgg acgcacttca ccagcatgac ttacattgat gagttttcag
     1861 agctgcatgg caaagatgtt ccggtgcgtg aagccctcgc cggacaagtg cccagcgcag
     1921 gcgtcggcac ctgtttcagc cgccgcgccg tgaccgctct gttagctgac ggtgacggta
     1981 ttgctttcga cgtgcagagt ctgactgaag attacgacat tggtttccgc ctgaaagaga
     2041 aaggtatgac ggaaattttt gtccgttttc cggtggtgga cgaagccaaa gaacgcgagc
     2101 agcgtaaatt tttacagcac gcacggacgt caaacatgat ctgcgtgcgc gaatatttcc
     2161 ccgatacctt ttcgactgca gttcgacaaa aatctcgctg gatcatcggc attgttttcc
     2221 agggctttaa aacccacaaa tggacctcca gcctgacgct gaactacttt ctctggcgcg
     2281 accgcaaagg ggcaatcagt aactttgtca gcttcctcgc gatgctggtg atgctccagc
     2341 ttttgctgtt gctggcgtat gaaagtttgt ggcccgatgc ctggcatttc ctttctattt
     2401 ttagcggcag cgcatggtta atgaccctgc tgtggctaaa ctttggcttg atggttaacc
     2461 gcatcgtgca gcgggtgatt ttcgtcactg gctactacgg tctgacgcag gggctgctat
     2521 ctgtcctgcg tcttttctgg ggcaacctga ttaactttat ggccaactgg cgcgcgctaa
     2581 aacaggtact tcaacacggc gatccacgtc gcgtcgcgtc gcgtcgcgtg ggataaaaca
     2641 acgcatgact tccccagcgt cactggcgat acccgctcgt tgcgcccgtt aggtcaaatt
     2701 ctgctggaaa atcaggtcat cactgaagaa caactcgata cagcactgcg taatcgcgtc
     2761 gaaggtctac gcctgggcgg ttcaatgctg atgcaggggc tgattagcgc cgagcagctg
     2821 gcacaggcgc tggcagagca aaacggcgtg gcgtgggaat ccatcgatgc ctggcagatc
     2881 ccttcctcgc tgattgccga aatgccggcc tccgtggcgc tgcattatgc agtactgccg
     2941 ctgcgtctgg aaaatgatga gttaattgtc ggcagtgaag atggtattga ccctgtttcg
     3001 ctggcggccc tgacgcgtaa agtcggacgc aaagtgcgtt acgtcattgt tctgcgggga
     3061 caaattgtca cggggttacg tcactggtat gcacgccgac gcggtcacga tccgcgggca
     3121 atgttgtaca atgcggttca gcatcagtgg ctcacggaac agcagaccgg tgaaatctgg
     3181 cggcaatatg tgccgcatca gttcctgttc gccgaaatac tgaccacgct cggtcatatt
     3241 aatcgttcag caattaacgt gttgttattg cgccatgaac gcagttctct gccgctcggc
     3301 aagtttttgg tcaccgaagg cgttatcagc caggaaacgt tggatcgcgt cctgacaatt
     3361 caacgcgaat tacaagtttc gatgcaatca ctattactca aagcaggttt aaacacagaa
     3421 caggttgcgc aactggagtc cgaaaatgaa ggagaataac cttaatcgcg tcatcggatg
     3481 gtctggttta ctgctgacgt ctttattgag taccagcgca ctcgcagaca atatcggcac
     3541 cagcgcagaa gagctggggc tgagcgatta tcgccatttt gttatttatc cccgtctcga
     3601 taaggcgctg aaggcacaga aaaataacga cgaagcaacc gccatccgcg aatttgaata
     3661 tatacaccag caggtgccgg ataatattcc gctgacttta taccttgcgg aagcctatcg
     3721 ccattttggt catgatgacc gggcgcggct gttgctggag gatcaactga aacgtcaccc
     3781 aggagatgcc cgacttgagc gcagtctggc ggctattcca gttgaagtga aaagcgttac
     3841 gactgttgaa gaactgcttg cccagcaaaa agcgtgcgat gctgcgccga ccctgcgttg
     3901 tcgcagtgaa gtcgggcaga atgccctgcg gctggcacag ttacctgtcg ccagagcgca
     3961 actgaacgat gcgacgtttg ctgcatcgcc ggaaggaaaa acgctgcgaa ccgatctgct
     4021 gcaacgggca atctacctga aacaatggtc ccaggcagat acgctataca atgaagcacg
     4081 ccagcagaac acattaagcg cggcagaacg ccgtcagtgg tttgacgtgc ttcttgccgg
     4141 gcagctggac gatcggatcc tggcactgca atcacagggg atcttcaccg atcctcagtc
     4201 atatattact tacgcgaccg cgctggctta tcgtggcgaa aaagcacgcc tccagcatta
     4261 tctcattgaa aataagccgc tgtttaccac ggacgcacaa gagaaaagtt ggctctatct
     4321 gttatctaaa tacagcgcca accccgttca ggcattggcg aattatacgg tgcagtttgc
     4381 cgataaccgc cagtatgttg ttggcgcgac gctaccggtg ctgttaaaag aaggtcagta
     4441 cgacgcagcg caaaaactgc tcgccaccct ccccgccaat gaaatgcttg aggagcgtta
     4501 tgctgtcagc gtggcgaccc gtaacaaggc tgaatctctg cgtctggcac gattgctgta
     4561 tcagcaagaa ccggcaaatc ttacccgcct ggatcaactg acctggcaac tgatgcagaa
     4621 cgagcagtca cgcgaagctg ccgatttatt gctgcaacgc tatcctttcc agggcgatgc
     4681 gcgtgtcagc cagactttaa tggcgcgact ggcgtctctg ctggaaagtc atccttacct
     4741 ggcaactcca gcgaaggtgg cgattttatc aaaacccttg ccgctggcgg cgcaacgtca
     4801 gtggcaaagc cagttgccgg gtattgcaga taattgcccg gcaatagttc gcttgctggg
     4861 cgatatgtcg ccttcctacg atgccgccgc ctggaaccgt ctggcaaagt gttatcggga
     4921 cacgctaccc ggtgtggcct tgtatgcatg gcttcaggcc gaacaacgac aaccgaacgc
     4981 ctggcaacat cgtgcggtag cctatcaggc gtatcaggtt gaggactacg ccaccgcact
     5041 ggcggcctgg cagaaaatca gtcttcacga catgagcaat gaggatctgc ttgctgctgc
     5101 caataccgcc caggcggcag gaaatggtgc agctcgcgat cgctggctac aacaggcaga
     5161 acaacgtgga ctgggaaaca atgccctcta ctggtggctg catgcgcaac gttacattcc
     5221 tggtcagccg gaactcgcac tgagcgatct cacgcgctca atcaatattg cgccttctgc
     5281 caacgcttac gttgcgcggg cgacaattta tcgccaacgt cataatgtcc cggcggcggt
     5341 gagtgatttg cgcgccgcgc tggaactgga accgaataat agcaacatcc aggcagcgct
     5401 tggttacgcc ttgtgggata gcggtgatat cgcacagtcg cgggaaatgc tcgaacaggc
     5461 gcataaaggg ctaccggacg atccggcact gatccgacaa ctggcctatg tgaaccagcg
     5521 tctggatgac atgcctgcga cgcagcacta cgcccgcctg gtgattgatg acattgataa
     5581 tcaggcgctg ataaacccac tcaccccaga gcaaaatcag caacgcttca atttccgccg
     5641 tctgcatgag gaggtcggtc gccgctggac gttcagtttc gattcttcta tcggcttgcg
     5701 ttccggggca atgagcaccg ctaacaataa tgtcggcggc gcagcgccag ggaaaagcta
     5761 tcgtagctac gggcaactgg aagccgagta ccgcatcgga cgcaatatgc tgctggaagg
     5821 cgacctgctc tcagtttata gccgcgtctt tgccgatacc ggagaaaacg gggtgatgat
     5881 gccggtgaaa aatccgatgt ccggcaccgg tctacgctgg aagccgctgc gcgatcagat
     5941 ctttttcctc gccgtcgaac agcagttgcc gctgaacggg caaaatggcg catccgatac
     6001 catgctgcgc gccagcgcct cattctttaa tggcggcaaa tacagcgacg aatggcaccc
     6061 gaacggttca ggctggtttg cccaaaacct gtacctcgat gcggcgcaat atatccgcca
     6121 ggatattcag gcgtggacgg cagattatcg cgtcagctgg catcagaagg tagctaacgg
     6181 acagactatt gagccttacg ctcacgttca ggacaacggc tatcgtgata aaggcactca
     6241 gggcgcgcag cttggcggtg tcggggtccg ctggaatatc tggaccggcg agacgcacta
     6301 cgacgcctgg ccgcacaaag tcagtctcgg cgtcgagtat caacatacct ttaaggcgat
     6361 taatcaacgt aacggagagc gcaacaacgc gtttctcacc attggagtgc actggtaaat
     6421 gcgtaagttc attttcgtat tgctgacact gcttttggtc agcccttttt cctttgcgat
     6481 gaaaggtatt atctggcaac cacaaaaccg tgatagtcag gttaccgata cccagtggca
     6541 ggggctgatg agtcagttac gtttgcaagg cttcgatacc ctggttttgc aatggacccg
     6601 ttacggcgat gcattcaccc agccagaaca gcgcgcgtta ttgtttaagc gggccgcagc
     6661 tgcgcaacag gctggcctga agctgattgt cgggctgaac gccgatccgg aattttttat
     6721 gcaccagaaa cagtcgtccg cagcgctgga aagctatctt aatcgcctgc tggctgccga
     6781 tctccagcaa gccagattat ggagcgccgc gcctggcgta acgccggatg gctggtacat
     6841 cagcgcggaa attgacgacc tgaactggcg cagcgaagcc gcccgtcagc ctttgctaac
     6901 atggttaaac aacgagcagc ggctgattag cgatgtttca gcaaaaccgg tttatatcag
     6961 tagttttttc gccggaaaca tgtcgcccga tggctatcgc caactgctgg aacacgttaa
     7021 agcaaccggc gttaatgtct gggtacagga tggcagcggc gtggataaac ttaccgctga
     7081 acagcgtgaa cgttatctac aggccagcgc cgattgccaa agctccgccc cagcaagcgg
     7141 cattgtttat gaactgtttg tcgccggcaa aggcaaaacc tttacagcga aaccgaaacc
     7201 ggacgcagaa attgcctcgc tgttagcgaa acgttcctct tgcggtaaag acactctcta
     7261 tttctctctg cgctatttgc ccgtcgcgca cggcattctc gagtattaaa tctcctccag
     7321 gtaagtcggg tacgacctgg cttacctctt tcgctctcgt cagaattcca tcaagattaa
     7381 ttgctaaaaa gcgtgctaaa gaaagaagat cgttatcggc gaacgggccg atcactcatg
     7441 caggaagcat agatcctttg agcggagttt ctgatatctg aggagtgcga aatgcaattg
     7501 agcagcagta aaccttgcgt ggtgatcctg accgaaaaag aggtagaggt aagcgtcaat
     7561 aaccatgcta cgtttaccct tccgaaaaac tacctggccg ccttcgcgtg caacaataac
     7621 gtcattgaac tctcaacgtt aaatcacgta ttaatcaccc acatcaaccg taacatcatc
     7681 aacgattatc tgttgttttt aaataagaac ttaacctgtg taaagccctg gtcgcggctg
     7741 gctaccccgg ttatcgcttg tcatagccgt acaccggaag tgttccggct agccgccaac
     7801 cacagcaagc agcaacccag caaaccctgc gaagcggagt tgacgcgcgc attgcttttt
     7861 accgtattgt ctaactttct tgagcaatcg cggtttattg ccctactgat gtatatctta
     7921 cgcagcagcg tccgcgacag cgtctgccgc attattcaaa gcgatattca gcactactgg
     7981 aatctgcgaa ttgtcgccag ttcgctatgt ttaagcccca gcctgctcaa aaagaaatta
     8041 aaaaacgaaa ataccagcta tagccagatt gtcacagagt gtcgtatgcg ttacgccgta
     8101 cagatgttat tgatggataa caaaaatatc actcaggtgg cgcaattatg tggctatagc
     8161 agcacgtcgt actttatctc tgtttttaag gcgttttacg gcctgacacc gttgaattat
     8221 ctcgccaaac agcgacaaaa agtgatgtgg tgaagggcaa agcggaaacg gataagacgg
     8281 gcataaatga agaagaaatg gcgcgccctg caggattcga acctgcggcc cacgacttag
     8341 aaggtcgttg ctctatccaa ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc
     8401 gcgtgaatta tacggtcaac ccttgctgag tcaatggctt ttgatctggt tgctgaacaa
     8461 gtgaacgacc gcgtctgatt ttctgattta tttcgctata gtggcaaaca aacgcacacc
     8521 gctgcgcgtc tgaatcaaga aaacccgtat tttcatgtat caaagtacaa tttaccgccc
     8581 taacggaaaa ttgtccgctc ctatgagact ggtaactatg aaaccaacgt cggtgatcat
     8641 tatggatact catcctatca tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga
     8701 attgcagatt gtcctgaaaa cggatgatta ccgcataacc atcgattatc tccgaacccg
     8761 tcctgttgat ttaatcatta tggatataga cttacccgga acagacggtt ttaccttcct
     8821 gaaaaggatc aaacaaatcc agagcacagt gaaagtgtta tttttatcat cgaaatcaga
     8881 atgcttttat gctggcagag cgatacaagc tggtgctaac ggttttgtca gtaaatgcaa
     8941 tgatcagaat gatatttttc atgccgttca gatgatcctc tccggataca cgttttttcc
     9001 tagcgaaacg cttaactata taaaaagcaa taaatgtagt acgaatagtt caacgatcac
     9061 tgtgctatct aatcgtgaag tgaccatatt acgttatctg gttagcggat tatctaataa
     9121 agaaattgcc gataagttat tacttagcaa taaaacagtt agtgcgcata aatctaatat
     9181 ttatggcaag ctaggtttgc attcaattgt agagcttatc ga
//
LOCUS       DAJOSY010000145         9204 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJOSY010000145 DAJOSY010000000
VERSION     DAJOSY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.145"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..245
                     /gene="yeaR"
                     /locus_tag="OHN27_003908"
     CDS             <1..245
                     /gene="yeaR"
                     /locus_tag="OHN27_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCQ2288267.1"
                     /translation="YPRLSVMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWH
                     NIEAMTDDTYFNIDFFVAPEVLMEGAQQRKVIHNGK"
     gene            249..431
                     /locus_tag="OHN27_003909"
     CDS             249..431
                     /locus_tag="OHN27_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCQ2288268.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            579..827
                     /locus_tag="OHN27_003910"
     CDS             579..827
                     /locus_tag="OHN27_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCQ2288269.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(886..960)
                     /gene="yoaJ"
                     /locus_tag="OHN27_003911"
     CDS             complement(886..960)
                     /gene="yoaJ"
                     /locus_tag="OHN27_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCQ2288270.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(963..1061)
                     /locus_tag="OHN27_003912"
     CDS             complement(963..1061)
                     /locus_tag="OHN27_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCQ2288271.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(1094..2119)
                     /gene="dgcP"
                     /locus_tag="OHN27_003913"
     CDS             complement(1094..2119)
                     /gene="dgcP"
                     /locus_tag="OHN27_003913"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCQ2288272.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            2302..2556
                     /gene="yoaF"
                     /locus_tag="OHN27_003914"
     CDS             2302..2556
                     /gene="yoaF"
                     /locus_tag="OHN27_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCQ2288273.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(2578..2925)
                     /locus_tag="OHN27_003915"
     CDS             complement(2578..2925)
                     /locus_tag="OHN27_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCQ2288274.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(2980..4161)
                     /gene="nimT"
                     /locus_tag="OHN27_003916"
     CDS             complement(2980..4161)
                     /gene="nimT"
                     /locus_tag="OHN27_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCQ2288275.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4258..5079
                     /gene="nimR"
                     /locus_tag="OHN27_003917"
     CDS             4258..5079
                     /gene="nimR"
                     /locus_tag="OHN27_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCQ2288276.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(5036..5482)
                     /locus_tag="OHN27_003918"
     CDS             complement(5036..5482)
                     /locus_tag="OHN27_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCQ2288277.1"
                     /translation="MFDVTLLILLGLAALGFISHNNTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            5652..5756
                     /locus_tag="OHN27_003919"
     CDS             5652..5756
                     /locus_tag="OHN27_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288278.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(5757..6260)
                     /gene="yeaK"
                     /locus_tag="OHN27_003920"
     CDS             complement(5757..6260)
                     /gene="yeaK"
                     /locus_tag="OHN27_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCQ2288279.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(6303..7793)
                     /locus_tag="OHN27_003921"
     CDS             complement(6303..7793)
                     /locus_tag="OHN27_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCQ2288280.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            complement(7974..>9204)
                     /locus_tag="OHN27_003922"
     CDS             complement(7974..>9204)
                     /locus_tag="OHN27_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCQ2288281.1"
                     /translation="KLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSL
                     AFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKD
                     NILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYT
                     KILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRG
                     IEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDF
                     CVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGMLFTEL
                     NSAQVKIIAERIRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKA
                     LREAKSEGGNKVIIHHI"
BASE COUNT         2557 a         2135 c         2211 g         2301 t
ORIGIN      
        1 tttacccacg cctttccgtt atgcatgggg cggtcaaata tctcggctac gctgatgaac
       61 acagtgcaga gcctgatcag gtgatcctta tcgaagcggg gcagtttgcg gtgttccctc
      121 cagaaaagtg gcacaacatt gaagccatga ctgacgatac ttatttcaac attgacttct
      181 tcgtggctcc tgaagtcctg atggaaggtg cgcaacaacg gaaagtcatt cataacggga
      241 aatgaatcat gggcaaagca acgtataccg tgaccgtcac caataacagc aatggcgttt
      301 ctgtcgatta tgaaacagag acgccgatga ctttgctggt gccagaagtg gcggctgaag
      361 tgataaaaga tctggtgaat accgtacgtt cttatgacac ggaaaacgaa catgatgttt
      421 gtggttggta attatcactg atcgttgatg gcgcaatttc tgcgccatcg caaaaaaaac
      481 ctccgtgatg ctcgtcacaa atcttgccct cccgatgttg tgctatgctt tttatcagcg
      541 actaacccgc taatgcaaaa cagataagtg aggaaacaat gggcattctg tcatggatta
      601 tttttgggct tattgccggt attctggcga agtggatcat gccaggtaaa gatggaggtg
      661 gattctttat gactatcctg ctggggatag tcggtgccgt agtcggcgga tggatcagca
      721 cgctgtttgg ctttggtaaa gtcgatggct tcaattttgg cagcttcgtg gttgccgtta
      781 ttggtgcgat tgtcgtgcta tttatctaca ggaagattaa aagttaacgc ttaaattgca
      841 caaaggctgc acacaggcag cctttgctat tttttagagg tgacgttacc accatcccag
      901 ctcagtgccg agtacgacaa taatcgccac acccatcata ataatcgttg tttttttcat
      961 ctttatgctc ccggggcagc atagccgcca ataaaaaacc atgctaaaaa tacgaccgct
     1021 gtaatgaaga ttacaaccgg aaaaataata ccgattcgca tgttatcacc aatcaaaagg
     1081 atgtgaatac gcctcaggaa tgtagcgctg gatgcgcgac aaaaggtgtt ttctgtttgt
     1141 gttttttctc ctgatacatc gcaatatcgg cagcatgcag ggcactgtct gcatcggttg
     1201 tttcaggatc gacttctact atgccaagac tggcaccggg ataatacaaa tcaacatcac
     1261 ctaagtgata ttctccacgt atttgctgct gaatgcgttc tcgcagcgac gaaatatccg
     1321 cattctcgtt gtttagtgaa acaactaaaa actcatcacc gccgagacga ccaataactt
     1381 cgccattttg ctggagcgta ttaaggcgct cgccaacctg aatgagaaac agatcgccac
     1441 tattatgacc aaaacgatca ttgattaatt tgaagttatc cagatcgata aacgcgatca
     1501 ttatcttatg gttaagatgc cgggcgaggg aaaacagtgt cgtcagattt tcaaaaatcg
     1561 cccgccgatt cggtagcccg gttaacgagt cggtatacga ttgcgcaatc agcgcagcat
     1621 tggcttcgcg cagctgttca accagtgcct ctttttgaat atattgtgca atcagtccgg
     1681 cgaataactg taaaacctgt tccgcgcgtt cactccactg gcgcttctca ctgctggcgg
     1741 cgcaaagcgt gccatagaat gatccatccg gtaagtgaat tggcgtgctc agaaatgtgg
     1801 tgatgccaag attgcgtgcc gcaatacagt caccccaacg gtcggggact tcatcgctga
     1861 aaaagcagtt ttcatcaatg gcgcgtttgc ataacgagta atcccacgag acggtaaaat
     1921 tctccgggat gtgcattttc tggctgttac gggcaaacat aatatgctgc aggcgcgctt
     1981 cgacatccac tttggtcagg taggttgatt ccatatcagt gaccatttcc agcatctcca
     2041 gaagctgtcg gaccagactt tccagtgact gttctttggc aagggattgc gagacgcggg
     2101 cgataatctg atctgacacg gaacaactcc aggatacggc gggtaaatgc tgattattta
     2161 atctttgcag gcatgataaa acatcatcaa caaagatgaa acaagacgta atatgttttg
     2221 agtgaatgta ataaaaatag tgctgcaaaa caaaacgata ccgcgatact ctaatcttgg
     2281 ataaatatgt gagatcaata aatgaaaatt atcagttttg ttctgccttg cttgctggtc
     2341 ctggctggtt gttcaacccc ttctcagcca gaagcaccta aaccgccgca gattggtatg
     2401 gcaaatccgg cgtcggtcta ttgccagcag aagggcggga cgctcattcc agtgcagaca
     2461 gcgcaagggg tcagcaacaa ttgcaaatta ccgggcggtg aaacgattga tgaatgggca
     2521 ttgtggcggc gtgaccatcc ggctggtgaa aaataaccgc cggatgctgt actaaaatca
     2581 caagctacgt agccagtcgg ccagcaccag cgcatggttc tgcgtggtgt tttttgctga
     2641 gtagagcagg gtcagcggct gttttttggc gatgtccgcc agccgctttc cttcttgctc
     2701 gtgttgcgcc agttctgcaa gatattgctc gcgaaaggtt gcaaaatcga cgacttcgcc
     2761 gtgaaaggct ttgcgcagtt ccgttgacgg cgtgatttct ttatcccact catcaagggc
     2821 taaatcggtt tttttgatac cgcgcggcca gaggcggtcg accagtatgc gataaccatc
     2881 gctctgttca gccggatcat aaacgcgttt gcactgaata ttcatcgtgt ttgttctccc
     2941 tttcacgcat gatttgttac gtcactatag caccggatat tagcgaattt ctttgtctct
     3001 cccggcgcaa agtccgaaaa tcgccatcag tagtgaaagt atggcaacac ccataagtgg
     3061 tacagaccag ttaccgttag catcgtgtat tttacccatc agcggcggcc cacaggctgc
     3121 caacaaatac ccgacggatt gtgccatccc cgagagtgcc gccgcctgat gcgcagaact
     3181 tgcccgaaga ccaatgaacg tcaaccccag tatcattgtt gcgccggaac caaaaccgaa
     3241 aagcagagtc caggtgatcg cgtgcgctgg cataaagcag agcccaacag cgcccactgc
     3301 gcacatcaag gcaacgaacg ctgcaatacc acgctgatct ttcacatgat gtaagaaaag
     3361 agggatcagc aaaccgggag ctgctgtggc tagttgcagc aaaccatgca gtgaacccgc
     3421 ctgtgcttcg ctatagccgt gactgatgag gatcgccgga agccagccaa taatcacgta
     3481 atagaccagt gagttgatcc caagaaacaa tgtgacctgc caggcaagcg gcgaacgcca
     3541 gataccccga gtatgtaagg cgcgcgaggt actcaaattt gcatgttgtt ggcttcgcca
     3601 ctgtggcagc cataaaaaaa gagccagcag aggaaaacac atcagcatga gcaacgcgcc
     3661 ttgccagcca aaaccgttca aagccagcgg cacgaccata gccgatccaa gtgccgccgc
     3721 agctcccatt gtcagggaat atgcgccggt aagtctggcg acggaatgag ggaaatcgcg
     3781 tttaattaat cctggcagta agacattgcc taatgcaatc ccaccgccaa tgaccgctgt
     3841 accgccaaat aataagtaag gcgaagggag agagcgaatt gcgataccag cacagatcaa
     3901 aagtaacgcg gcaaacaggc tacgttccat accaaaacgt cgcgctaccg gggcggccaa
     3961 aggtgagatt agcgcaaagg ccaataatgg cagggtggtc aataagccgg tttgcgctgt
     4021 cgtcagcgag taagcggaac gaatcgtatc gagtaacggt gctgcgccgg tgaaggtgac
     4081 gcgtaatgtt gtggcaatca tcagaatgcc agcgataagg acaatcctgt ttttgccgct
     4141 taatgaagtt gaacaggtca tatgcttctc attgttacaa acgaggctgt accttaccgt
     4201 ttttattgtt atgttgaatc aagctaaaat gacaaattat cgctaaattc ggacaaaatg
     4261 atgcacaggt tgaatctcaa tggctatgaa cccgaccgac accatgaagc cgcggtcgca
     4321 ttttgtattc acgccggaac ggatgagtta accagtcctg tacatcagca ccgcaaagga
     4381 cagctgatac tggcgctaca tggtgcaatt acctgtacgg tggaaaatgc tttgtggatg
     4441 gtgccgccac aatatgcggt ttggataccg ggcggggtga agcatagcaa tcaagtgaca
     4501 gctaacgctg aactctgttt tctgtttatc gaacccagcg cagtgacaat gccgacaaca
     4561 tgttgcacat tgaaaatatc gcccttatgc cgggaattga ttttaaccct ggcaaacagg
     4621 acgacgactc agcgtgctga gccgatgacc cgcagattaa ttcaggtcct gtttgatgaa
     4681 ctccctcagc agccacaaca acagctgcac ctgcccgttt cttctcatcc taaaatccgc
     4741 acgatggtgg agatgatggc gaaagggccc gtcgagtggg ggtcattggg gcaatgggct
     4801 ggcttttttg cgatgagtga acgcaatctt gcccgactga tagtaaaaga gacggggttg
     4861 agttttcgcc agtggcgtca gcagttacag ctgattatgg cgcttcaggg attagtgaaa
     4921 ggcgatactg tacagaaagt tgctcatacg ctggggtatg attcaacaac ggcctttatt
     4981 actatgttta agaaaggcct ggggcaaacg ccaggccgat atattgcggg attaactact
     5041 gtttccccac aatcagcgaa accagacccg cggcaataag cgggccaacc ggtacgccgc
     5101 gaaacagcgc cacgcctaaa acagtgccta ccagcagtcc ggcgaccagt tgcggctggc
     5161 tgcccattaa cgtcacgccg cgcccaccca gccaggagac aataacgcct actgcaattg
     5221 ccaccagtga tttccagttc agaaacgagt gaatcagggt tgagggcggt agcgtcccgc
     5281 tggcgatggg ggccataacg ccaatcgtca gaatgattat cccgatactt agcccctgtt
     5341 tttcaatcca cgggaaaaaa gtgcttaacg gtgtgacgcg gacaatgatt aacaccagaa
     5401 ttgaaacggc gacagtgttg ttatgactga taaagcccag cgcagctaac ccgagcagga
     5461 tcagcaaagt gacatcaaac atcgtggttt ccttgccaaa aaataataag cctgcttact
     5521 ttacgcttgc tgcaggaagg aagtagaaaa tctttacgtt ttttgcataa aaaatcaggc
     5581 gaactgtcat caaactgcca tttgtgcacg ctaacaataa cgtgaaaaca gctatctccc
     5641 gggggcatgc tatgaacgat caaatgttcg tcgagacact gattatcacg tcatcgtttt
     5701 ttgccattgc tgttgtgctg gttttgtccg ttctgcttat tgaacggaca ggctagttac
     5761 gcagtgcggc ggaaattgag cagttctggt tgcgcaatgc gcaaataatc cgtggttttc
     5821 aaaataacgg atttatccag catccctgca ttgaaggcga tttcatcaaa tcgttcgaag
     5881 agtagagggt cggcaaccag tttgagtttt ggatggaagc tgaaaggggg gatcgcgccg
     5941 aagacacagc ctgtgagttc atcgacttca gcagggctgg ctaacgaagc gcgtaatccg
     6001 cctatatggc tggcaagttg gctcagatcg gcctgttgat cggcagcgag gatcgccaga
     6061 acatgttgat ttacgccatt gcctttgact ttacatacca gtgccttagc tccctggcct
     6121 aaagcggtgc cacgaatttc tgacaccgct tcacatttac ctaccgcttc atgggtaacg
     6181 acgcggaagt tagcaccttc ttgtgataat aaagcgatta accgctgatg cgtcacgctt
     6241 cctttagcca tttcagtcat ttcacgctcc tgagattaca agcaaacaac cacagaaggt
     6301 tatcatgaac ggctgttttt gttctgctta ttgacatata aacgctcatc ggaagcttta
     6361 tacgcatcat gtaacgtatc gttttctttc atcgcgtaaa taccggaaga gaagccgatc
     6421 tctttctgcg gcgcgatatg ttgcagacgt ttttcgatac gttcaggcag ttgtgctgca
     6481 atttgcggcg tcgaatcgac aagaatgatg cagaattcat cgccaccgag tcggatggca
     6541 taatcacttt tacgaatcga ttgtttaatc gcctgagcta acaacgtaat cgctaaatcc
     6601 ccttcctgat gaccgagggt gtcatttatt agctttaact tgtccatgtc aattgcaata
     6661 aacatcaccg aagaaccgga ttgcaccagt ttctgcaacc gctgctccag ttcaggggtt
     6721 aaaattttgc gattatacag tccagtcatc gcatcactaa tattttctcg cgacacattt
     6781 tggtataagc ggaagtgcat ccgcaccata ttcagcaaaa ttaacgccgt caaaatccag
     6841 aacagaacgc ttttccacga agacgtgata aagtaaagaa tatcaatgga taacgagaca
     6901 cgaatgccgc ccggtaagtc atggacgtaa ctgacatact ggaacagatt atcttcgctc
     6961 tggttgatga taatgtcgcg ccccgaatcg gtatcggtta gcgtgacatt gagaaaacgc
     7021 cagaggagag ggcggtcatg agtgtaaaag atattccgca aattgttttt gttaatatcc
     7081 agcagcacaa tcccttttag ctgccctgcg acatataccg gcgtcagcaa actcatcacg
     7141 ttctgtttag tacgctgatc ctggtaaatg ctcgacaata cggtacgccc ggtaaatacc
     7201 ttttcaatat ctttgcgatc gatgccaata cttcctgact gcaggaagga ccagttattc
     7261 atcgcaaagt attcaacatt aaccggctca taaaaataga catagcggtc attgagatcg
     7321 agataatagt gaaatttctc ttcggccatc ccaagacctg aaccgtaatc atcctggtgg
     7381 cggttgcctt ccactgcctg atcaaaagcg ggtaatagtg ctgcagcttc cacaattgtg
     7441 tcgcagtcag tggatgcggt ttgtagtgtg ccgcgtaagc ccgggtattt atgggcagtc
     7501 aggtttaagc catgggtatt attggcagac tcaaaagcct cgcagatggc gcgccgctgt
     7561 tctggcgaca cttccgactg ctcagcagcg agagcgcgca tcacatgcgc ggcaatactc
     7621 tgattctgat acttatcata aagaaaggag gagtctgctt tctgcacgat atagcgcaga
     7681 taggcactca ttgctctgtc gctggcgaca agttcaaaaa taaggaagga agatgtcagc
     7741 acaatgacac ttgcggcgat aaaatgccgg agcattctat ggtgcaattt catgataaat
     7801 ggcactcctt cggttagtga aacgagaaaa ccagataaag ttcgattgcg gcctggcaca
     7861 ggggcgctaa ttatactcat ctctgcgccg ctggggaggg gaaatttagg ggtaatagta
     7921 ataaattgta tgcaaaagcc acgcaaaaag catggctctg aatatttttg cacttaaata
     7981 tgatgaataa tcactttgtt gcccccctcg cttttcgctt cgcgtaaggc tttatctgct
     8041 tccgtcatga caagcgagat attacgcgtg tcacccgttg aaaaaaccgt gccaatactg
     8101 atggtcattt gttcaggaac atcatatctg ttactaaagc cggtcaggag ttcgacattt
     8161 ttacgtatac gttccgcaat gatttttacc tgggcactat tgagttcggt aaatagcatg
     8221 ccaaacacct caccctccag tcgcgctaaa atatcatcgg ggcggatgct ttgctggata
     8281 atatcgacaa ttgaaaccag cactttatca cccacacgat gtccccattg ggcattgatg
     8341 cgtttgaact gattaatatc aaccagcatg acacagaagt ccttaacaac aggtcgcgac
     8401 aataatgact ctacgctgtt gaaaaagtag cgccgattat aaatattggt cagcacatca
     8461 tgaactgcca gtttgctgga gagttgcagt tcctgaaaaa tgttataaat cagaaaagac
     8521 acaacaaaca gtttactgga gacttcaatc ccgcgactga tgtaccagat ggtgtaatta
     8581 tactcatcca gagtcagtag aagtaagttg cagcatagcg atgccagaca taataatgct
     8641 attaatggcc atagttcgct ggctaatcgt gtacgcatga taataaagaa cagtaaaaat
     8701 gcccacagac aaaccaatat ttttgtatag ttgattcccc aggtcgcagt atggttgtca
     8761 ggacagtagt cgacgacata caaagaatag tcagcgttat aactgctcag attgtgtgcc
     8821 agaaggggaa aaactaaaaa aggaatcagc gccagcaaca ggattcccgt ttttttctta
     8881 ttgttgtcaa ggatgttgtc ttttccataa caaaagagtg ccagcgaggt taaacaaata
     8941 aaactgagtt ggcggaaaat atagaaaata gaaacatcat tcgactttgt ctggatcagt
     9001 gtactgccgt taattggttt atggataatt acaatggttt caactaaata gataaggcca
     9061 cttaaaaatg caaggcttaa aatgagtgtt ggtaaaattt gtttgttagt gaaatatttc
     9121 attaacatga aaagaaaaat gacggcgtta aggaaaaaca aagccacaac aaccatcagg
     9181 taattcatct gtgggatgag tttt
//
LOCUS       DAJOSY010000146         8785 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJOSY010000146 DAJOSY010000000
VERSION     DAJOSY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.146"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            268..1098
                     /locus_tag="OHN27_003923"
     CDS             268..1098
                     /locus_tag="OHN27_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCQ2288282.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDSELTRKYECARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1239..2012)
                     /gene="uxuR"
                     /locus_tag="OHN27_003924"
     CDS             complement(1239..2012)
                     /gene="uxuR"
                     /locus_tag="OHN27_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCQ2288283.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSSDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(2227..3687)
                     /locus_tag="OHN27_003925"
     CDS             complement(2227..3687)
                     /locus_tag="OHN27_003925"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCQ2288284.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGAYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3768..4952)
                     /gene="uxuA"
                     /locus_tag="OHN27_003926"
     CDS             complement(3768..4952)
                     /gene="uxuA"
                     /locus_tag="OHN27_003926"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCQ2288285.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5292..6635
                     /gene="gntP"
                     /locus_tag="OHN27_003927"
     CDS             5292..6635
                     /gene="gntP"
                     /locus_tag="OHN27_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCQ2288286.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATDQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6808..7710)
                     /gene="fimH"
                     /locus_tag="OHN27_003928"
     CDS             complement(6808..7710)
                     /gene="fimH"
                     /locus_tag="OHN27_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCQ2288287.1"
                     /translation="MKRVINLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVVIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIAVTFVYQ"
     gene            complement(7730..7813)
                     /locus_tag="OHN27_003929"
                     /pseudo
     CDS             complement(7730..7813)
                     /locus_tag="OHN27_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            7868..8307
                     /locus_tag="OHN27_003930"
                     /pseudo
     CDS             7868..8307
                     /locus_tag="OHN27_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    8149..8265
                     /locus_tag="OHN27_003930"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(8491..8748)
                     /locus_tag="OHN27_003931"
     CDS             complement(8491..8748)
                     /locus_tag="OHN27_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCQ2288288.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2169 a         2225 c         2153 g         2238 t
ORIGIN      
        1 ccattcacag gcattattaa tatagacatc aatatagcaa catcaaattc caattagtaa
       61 taagatatgc ttttaaattg catgaaaata atttcaaatg agcgtttttt gatactttag
      121 agtgtttcct accctttaat atcaccgtgt agagttgata tcactacaac agaaacatgc
      181 atgtcatttg tttacttctc attttactac agaaaaattt atctattttt ctaaacatca
      241 gtgaaatact cataaaaggt aactattatg tctatgccat taagcaatac tcttcagagc
      301 caaattatta cagataatca cttccttcat catccaaaag tcgacagtga gttaacacgt
      361 aaatatgaat gtgcacgcct ggatacggaa aatatctatc ttctgcctct tgctcgtggc
      421 aataaccata attacgatgg aaaaagcgtt gtcgaaatta gaaaacttga tattagtaaa
      481 gagtcttggc catttaatta tgtgacagaa gcatgcagag aatccgatgg tattacaaca
      541 acaggacgta tgctctatcg aaacttgaaa atcacctcag ctttagacga aatatatggt
      601 ggcatatgta aaaaggcgca tgctgctaca gaattagcag aaggtttacg gctaaattta
      661 tttatgaaga gccctttcga tcctgttgag gactatacag ttcatgaaat aacattaggc
      721 cctggttgca atgttccagg ttacgccgga accacgatag gatatatttc aacccttcca
      781 gtttctcagg caaaaagatg gaccaatgag cagccaagaa ttgatatcta tattgatcag
      841 ataataactg tctcaggggt agcaaattct tccggattcg cccttgccgc attacttaat
      901 gctaatattg agatgggaaa tgacccaatc attggtatag aagcctatcc tggtaccgcg
      961 gagatccatg caaaaatggg atataaagtt atccccggtg atgaagatgc gccactcaaa
     1021 cgcatgactc tgcaaccgtc atctcttcca gaactgtttg aattaaaaaa tggtgaatgg
     1081 aattatattg gtaaatagcc actgagaaga atgagtacta atattcaggt tatacagata
     1141 tttaaacaat accctgcaat ttacaaagca atactcattg cgacttctta atttaagaaa
     1201 gcgcaaggaa cgtttaccct tgcgcttatt ataataagtc agacgtcgac tttatccagc
     1261 ggccatgaat caaacagata gccgtcaaaa taaatatcgt caacgttcga gaattccagc
     1321 agacgttgct taacgttttc cagatgctgc cacattgcca gcttcgccgc tcgggcatct
     1381 tttttgatta acgcggcgag gatctgtttg tgatcgccca accactcttt gcgatacagg
     1441 ctgtcatcca gatggctgtg caactggatc cacattggat tgttttcccg ccactgccag
     1501 gactgacgga ataactccac cagcatgctg ttatgcgttg cttcggcaat ggcgagatgg
     1561 aactgcatat caccgctttc gctgctaccc ggcgcgctgg aagccagttc acgctcttcc
     1621 agttgcaatg cctgacgcat tttgacgata tcttcgcggg tagcctgcaa agcggcaaat
     1681 tccgcgatgt tactttccag taactgacgc gcctgtaaca gctcaaaagg acccgcatca
     1741 ttacagacgt tggcatccga actgtctgtg ttgtggctgc ctgagctatc aagaacatag
     1801 ataccggcac cccggcgcac ttccaccagc cctttgatct ccagcatgat cagcgcttca
     1861 cgtaccaccg tccgcgtgac atcaagcatt tctgcaattt cacgctccgg cggcagccgt
     1921 tcgccaggat tgtacggcgt ctttatgatc agatcgcgga tcatcgcccc gacttcctgg
     1981 taaggtcttt gcgcagaggt ggcagatttc atggtgttgt gtccgttagg tgcggttaat
     2041 caaatttttg ccggatgcgg cgtgaacgcc ttatccggcc tacaacgggc aattttacgg
     2101 cccctgttaa tctcaaacta taccattagc actggaagtt ggcacgtaca ggtgggcaaa
     2161 aaaacgaaaa ctggtcaacc aaatatcagt ttgattgacc agtaagtctg tagggtaatc
     2221 agttagttac agcgcagcca cacactcgcg cgcaccgcgt tcgcacagct gctgatatgc
     2281 cgccgtcacc gcgccaacaa agtcggcatt ctgcggcaga tcatcggcaa aaataccgct
     2341 caggcctagc agcgctttca cgcggtctgc gccctgatac tgcgcgttaa tcttctggaa
     2401 ctcggccagc atcgggtcga ccacgtcaat ggcattaccc tgctcatcca cgccctgggt
     2461 gtaacgcatc cagccagcca cgcccagcgc caggtgacgc cagctgccgc cgttttgcag
     2521 gtgcagacgc accgggtcca gcagacgctg cggtaacttc tggctgccgt ccatcgcaat
     2581 ctgccaggta cggtgacgca gagacgggtt gctgaaacgc tcgatcagca gcgtcgcata
     2641 ggcgttcagg tccgtacctt ccggcatcga cagcgttggc gcttgttcct gcatcatcag
     2701 ggcaaaggcc gctttgcgat aatccgggtt agtcatggtg tcggcaatag tttcatagcc
     2761 gccgaggtaa ccgaggtacg ccaggaaaga gtggctaccg ttcagcatac gcagcttcat
     2821 catttcgaac ggcacaacgt ctgcaacgaa ctgtgcgccc actttatccc agtccgggcg
     2881 accattaacg aaattatctt caatcaccca ctgacggaac ggttcgcagg caatggcgca
     2941 cgggtcgtaa gcgcccagct ggtcagcaat ttcctgtaag gtttctggcg tcgccgccgg
     3001 aacgatgcgg tcaaccatgg tgcacgggaa ggtgacgttc tcttcaatcc atgccgctag
     3061 ctgcgggtca cgcgcctgag ccagccccag taccgcgacc ttcgccacat gaccgttttc
     3121 acgcacgtta tcgcaggaca tcaccgtaaa tgctttcagc cctttttcac gacgcagacg
     3181 caaggcttcg acgatgtaac caatcgcgga cttcggcgca gtcgggtttt ccagatcgtg
     3241 cttgatcagc gggttattga gatccagttg accgcttgcc gcatcagcgc agtagccttt
     3301 ttccgtgact gtcagagaga caatcgccgt ttgcggacgc gccatcgcgt tgagaatacc
     3361 ttcgcagccg tcgatttccg ggtgcagcgc ttctttcatc gaaccgataa ttttcagctc
     3421 ggtgctttcc gcgccttttt ccgccacggt gtacagcagt tgctgttttt tcaggttttc
     3481 gatcagcacg cggtcgttgc ctggcatcag gttaacttcg cagatacccc agtcgctgtc
     3541 ggtgctttcc agcagatgat gggtgtacag cgcctggtgc gcacggtgaa acgccccgca
     3601 accgagatgc acaatgcgtg attccagacg agaatgatcc catgacgggc gggcaaccgg
     3661 cagattgctg tcaacaatag tagtcatcgt aaactccaga atccgtcttc agagagtgac
     3721 ggcaaggaag aaggaaccgg cacggcgcgc agccatgccg gtggatatta acggctaaag
     3781 aaagcgcgct ggatcgccag ttcgacaccg cgaacttcgg ccaggccttt cagacgacca
     3841 attgcggagt aacctgggtt ggttttcttc ttcaggtcgt ccagcatctg atgaccgtgg
     3901 tccggacgca tcgggatcag gtcttctttg ccttccgctt tacgacggtg ttcttcttca
     3961 acaatcgctt tcaccacttc gtacatatca acgtcaccgt tcaggtgcgc cgcttcgtgg
     4021 aaggttttcg ggttatcttc acgcatggtg gagcgcagat gggtgaagta aatacgcgga
     4081 ccgaactgct tgatcatatc aaccagatcg ttgtcagcac gcacgccgta ggaaccggtg
     4141 cacatggtaa aaccgtttgc catgctgttt acggtatcaa ccatccactg catatcttca
     4201 atggtagaaa caatgcgcgg caggccgagg attgggcgcg gcggatcgtc cgggtgaaca
     4261 gccatacgca cgccaacttc ttcggcaacc ggaataatcg ccttcaggaa gacggcaaag
     4321 ttttcgcgca gtttggcttt gtcgatatct ttgtacagct ccaggtgctt acggaactgg
     4381 tcgagggtgt acccttcttc cgcgcccggc agaccggcaa tgatgttacg ggtcagacgc
     4441 gctttgtctt cgtcgctcat agtggcgaag cgaacagcag cctgagcaat ttcttcttcg
     4501 gtgtaatccg cttccgcacc tgggcgtttc aggatatgca tttcgaatgc agcgaattcg
     4561 atctggtcga agcgcagagc tttggagccg tctggcagca cgtattcgag gtcagtacgg
     4621 gtccagtcga gcaccggcat gaagttgtag cacacggtgc gaataccgca ctgcgccagg
     4681 ttgcgcagag tctgctgata gttagcgatc cactgctcat agttgccagt gtgggttttg
     4741 atatcttcgt gaattggcac gctttctacg acagaccaca ccaggcctgc atcttcaacg
     4801 atagccttgc gtttgaggat ctcttctacg gaccatactt caccgttcgg gatatggtgc
     4861 aaagcggtaa ccacgccagt cgcgcccgcc tgacggacat cagctaaaga aaccggatcg
     4921 tttgggccgt accagcgcca ggtctgttcc atgatttttc ctctttcaca tagtgggttc
     4981 cacagcaaag agacgcggaa ccctggagtt gtttaggtat ccggatgaac aaccagaccg
     5041 cagccgtggc gttgcatcgg atgagatggc gtataagttc taccttttgg taagacatgt
     5101 caacccgaat tctgaaattg gttaaccaca tcacaagaat ttcactctcc tggttgttgc
     5161 tgcaaattgc agcaaaaggt aatcgatggt tattgatcag aggatgtgac attcatcgca
     5221 acaatggttg accaatttac ataacatgtc gcccaaaata acaacggttc aaccaggtaa
     5281 ggtaaaacgt tatgcatgtg cttaacattc tctgggtggt attcggcatt ggtctgatgc
     5341 tggtactgaa tttgaagttc aaaatcaatt caatggtggc cttgttggtg gcggcgctgt
     5401 ccgtcgggat gctggcgggc atggatttga tgtcgctgct gcacaccatg aaagcgggct
     5461 tcggcaacac gctgggggaa ctggctatca tcgtggtgtt cggtgcggtc atcggtaaat
     5521 tgatggtcga ctccggcgcg gctcaccaga tagcgcatac gctgctggca cgtctcggtc
     5581 tgcgttatgt acagctgtcg gtgattatca tcggcctgat tttcggtctg gcgatgttct
     5641 atgaagtggc ctttatcatg ttagcaccgc tggttattgt tattgccgcc gaagctaaaa
     5701 ttccgttcct gaaactggcg atcccggcag tagcagctgc cactaccgca cattcactgt
     5761 tcccaccgca gccgggtccg gtggcgctgg tgaatgctta tggcgcggat atggggatgg
     5821 tttatatcta tggcgtactg gtgacgatcc caagtgtcat ctgcgcaggt ctgatcctgc
     5881 cgaagttcct cggcaatctt gagcgcccaa cgccatcatt cctgaaagca gatcaaccgg
     5941 tagatatgaa taatctgccc tctttcggcg tttcgattct ggtgccattg atcccggcga
     6001 tcattatgat ctctaccacc atcgccaata tctggctggt aaaagatacc cctgcgtggg
     6061 aagtggttaa ctttatcggt tcctcgccga ttgcaatgtt tattgcgatg gtggttgcat
     6121 tcgtactctt tggcaccgcg cgcgggcacg atatgcagtg ggtaatgaac gcttttgaaa
     6181 gcgcggtgaa aagcattgca atggtgattc tgatcatcgg tgcaggtggc gtgctgaagc
     6241 agaccatcat cgacaccggc atcggcgaca ccatcggcat gctgatgtcc cacggcaata
     6301 tctcgcccta catcatggca tggctgatca ccgtactgat tcgtctggcg acgggtcagg
     6361 gtgtggtttc agcgatgacc gccgccggga ttatcagtgc agcaatcctt gatccagcaa
     6421 ctgatcagct ggttggcgtg aatccggcgc tgctggtact ggcgacggct gcgggttcca
     6481 acaccctcac ccacattaac gatgcctcat tctggttgtt caaaggttac tttgacctgt
     6541 cagtaaaaga cacattgaaa acctggggtc tgctggagct ggtcaactcc gtggttgggc
     6601 tgattattgt gctgattatt agcatggtag cgtaaaagag cacctgagcc tgccattggc
     6661 aggctctgtt cggattgtcg gtaaagtgcg ctttgtttat gccagatgcg acgctgacgc
     6721 gtcttatctg gcctacaaag ggctaacgtg caggttttag cttcaggtaa tattgcgtac
     6781 ctgcattagc aatgccctgt gatttcttta ttgataaaca aaagtcacgg caataatcga
     6841 ttgcacattc cctgcagtca cctgccctcc ggtacgtgcg taatttgccg ttaatcccag
     6901 acttaccgcc gaagtcccta ctgctcctaa cgataccgtg ttattcgttg gaataatcgt
     6961 accgttgcgc gtcaactgta cgccgacgcc ctgtgcaggt gaaaacgacg cggtattggt
     7021 gaaaatcgag ttgcccgcat ctgcggttgt gccggagagg taatacccca ggttttggct
     7081 tttcgcacaa taaacggtaa gaggaatcgg cactgaacca gggtagtccg gcagagtaac
     7141 ggtgacatca cgagcagaaa catcgcagcc gccagtgggc accaccacat cattattggc
     7201 gtaaatattc cacacaaact ggaaatcatc gctgttatag ttgttggtct gtcgcaaaat
     7261 aagcacggca attaatgagc cagctttaat caccaccccg cccgcactgc tcacaggcgt
     7321 caaataaagc gccaccggcc acggcttatc cgttctcgaa ttataaacaa cccgcggcgt
     7381 ttcactggtg gtcggaaatg gatagctact gccactatat tttacggtcc cggaaaaatt
     7441 agataacacg ccgccataag ccgagcctcg ttgcagtgtg acataatctg taatggtttc
     7501 cgggtaatcg ttatggcaaa agatttgcgt cgaaagatcc acgaccaggt tttgccccac
     7561 attcacggcg ggcgcaaggt ttacataaac attggcgctg ccaccgccaa tagggatagc
     7621 ggtgccattg gcggttttac aggcgaatga ccaggcattt accgaccagc ccatcagcag
     7681 tacagcaaac aggttaataa ctcgtttcat tacaatcatc tcttcggctt cagctgtagg
     7741 tataggtgat gctaatcact gcctgaatgg ttccctgagt ggctccgcca tttaccgtca
     7801 atgctctgac ctgtgaaccg ccccggaaat cctggagact aaacttcctg agaaagaggt
     7861 aaacaggatg actaaaaata ctcgtttttc ccccgaagtc cgtcaacggg cagtccgtat
     7921 ggttctggaa agtcagagcg aatatgactc acaatgggcg acaatttgtt ccattgctcc
     7981 aaagattggc tgtacgccgg agactctgcg tgtctgggtt cgccagcatg agcgggatac
     8041 cgggggcggt gatggagggc tcaccaccgc tgaacgtcag cgtctgaaag agctggaacg
     8101 tgaaaatcgt gaactgcgcc gcagtaacga tatccttcgc caggcttccg cttattttgc
     8161 gaaggcggag ttcgaccgcc tctggaaaaa atgatgccac tgctggataa gctgcgtgag
     8221 cagtacgggg tcggaccgct atgcagcgaa ctgcatattg ccccgtcaac gtattaccac
     8281 tgtcagcaac agcgacatca tccggatatg cccatgatac caaaatgacg aaagccgaaa
     8341 tgtctgaaag cgtgtgtatt acctgaaaat atgaccggct ccggggtacg taaccgattc
     8401 aacaaagcca atcgatatta caaaacgaga gggactatat atttcgtttt cagataagac
     8461 tccttgagag attatctttt atgtaaaatc tcaaatatca cctgatgtaa tgtcaatgat
     8521 aatattaagt gcgctctgaa caacatcttt cttggtgtga ttaacatctg acaaaaagaa
     8581 catcagcttc tttattatgt tcttgttgtt tattatttca cgctcacaga ataaagcgtt
     8641 gactgcctct tctatgacat ttctctcgta aaaaaattga gagttagctt catataaaat
     8701 attggccaat ttttcaccaa aaaccatatt ataataatca ctgtgcatct ttctccccca
     8761 gggttcagtc aataatttta ccatg
//
LOCUS       DAJOSY010000147         8710 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJOSY010000147 DAJOSY010000000
VERSION     DAJOSY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.147"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..240)
                     /locus_tag="OHN27_003932"
     CDS             complement(<1..240)
                     /locus_tag="OHN27_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288289.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIKEGGAVSFIFNEE"
     gene            complement(218..3838)
                     /locus_tag="OHN27_003933"
                     /pseudo
     CDS             complement(218..3838)
                     /locus_tag="OHN27_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            3834..5699
                     /gene="tssM"
                     /locus_tag="OHN27_003934"
                     /pseudo
     CDS             3834..5699
                     /gene="tssM"
                     /locus_tag="OHN27_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            5710..7062
                     /locus_tag="OHN27_003935"
     CDS             5710..7062
                     /locus_tag="OHN27_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCQ2288290.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            7086..7568
                     /locus_tag="OHN27_003936"
     CDS             7086..7568
                     /locus_tag="OHN27_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCQ2288291.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            7612..8526
                     /locus_tag="OHN27_003937"
     CDS             7612..8526
                     /locus_tag="OHN27_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288292.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
BASE COUNT         2001 a         2485 c         2201 g         2023 t
ORIGIN      
        1 ttcctcatta aaaataaaac tcactgcccc cccctctttt attttaattt ccgcttcatt
       61 tgtgttcaaa tcaacttttt taattactcc atgtaagttg ggttttgata gtttttctac
      121 gccaccatca ggagcgggta atatacatcg tttaagaact ttagcatttg cacatccaat
      181 aaccaaaatt agaatgcaca ctcccatgtt aatcgcatta acgtgtctta aaacgttcat
      241 atctaattct ctctccagga tgcgaataca tccaacttgt cactttctta cacaactcat
      301 ctacatcttc attctgtgca cgggtacatc ccatagttgg tttccagcct tgtcgaggag
      361 cataaggatc gcgcaacgat gatcccccac catgtagcca tcttgctcta gtatctgtag
      421 tatagatctt cgttgtgcca tatgctgccg atattcctga ttcatttgtc tcacaaccaa
      481 cgacttcacc tgtatatgga tcaccagcac cacgttgaga acgacgatca attctattgc
      541 tactatcaaa agtcattgaa tcacctgttt caggatcata ataaatagtc actcctccag
      601 tcatatcagt aagaacaaga ggagtttcag cacctgcccg gtctggagcg ggaggcccat
      661 atacacttat aactcgttct aaccccagtg gatcagtgta acgtattggt gccagtggat
      721 acgtgtatag atttattccc cccttcaacc ctatcggatc ctgggtgata taccgcccct
      781 gcaacggatc gtagtaccgg tgacggttgt aatacagtcc cgactcctca tcatactgct
      841 gccccggcaa acggtacggc tggtacaggt gatgcgggtt ctcctcatta agctggttcc
      901 cccactcgtc atactccccg caccacgccg tttccccttc cgggctgatg agcgcctgcg
      961 gcagtccccg gtggtcacag tggtaaagat gcagcctccg ctccgggatg tattccgcct
     1021 ccatctggct ttccatctgc tccaccgtca gcccgcactg cgcaagccat gcctcactct
     1081 ccgcactcac cgctcccgca cgcagttccc gctccagcct ccccagcatc accgccagct
     1141 ccgccggaag cgtcaccccc gtgtcctcct gcaacacctc cgccaggctc cggtgccgcg
     1201 ccttcgcctg ttcgccattc tctgtttcga ttctcaggag cggcgtgaag cttcccggct
     1261 ggtataccgt ctggattctt gtctgttggg tctgtacagt ggtcagcctg tcaccgtccc
     1321 acccgtacca ggtctcctcc ggtttacgcg acagcgacat ccaccccgtc aggtcacgct
     1381 cccgccgcca tacccgtttt cccgtccggc ggcccagcgg gtcatagagg taccggctct
     1441 ccacctgtgg ttcgccgtgc tgtatccgcg tgtagaacac cagacggtgc tggctgtcgt
     1501 aatggtagtg gtgggtccgc tcgtcgtgca tccggataac cccttccggg atgaggtccg
     1561 tcttctccgc cagcctgccg tattcatcgt ggcggtagac atagtgcgca tcttccgcga
     1621 tgcggttatc cggccacgcc gtgagcgtgc tgtccgggtg cagttccggg tccggcagcc
     1681 ggtttcctgc cgggtccgtc gcatacggga tatcgatatc caggttcgcc gccgtggtgt
     1741 ggacgcccgt cagccttccc gtgtcactgt aacggtactc ccggctctcc tgcgggccgc
     1801 tgatgcggat tagctgtccg ttgtcgttcc agtcgtagtc ccggtcaagc tgcgggaggt
     1861 tcaggtgcct gctctgtagc tgccctgtga gggtgtatgt gctggtcagt tcatatgctg
     1921 cattactgcc tgccatgctg ccgaagctgc gcaccgtctc acggtgcagc cggtcccgcg
     1981 tgtactcgac cagcggtgtt ccgcccagct tcatccccgc aagataaccg ctgccatacg
     2041 tcagccactc caccggcggc agaccgtccg gctcctgacg ggtcgccagc ccctgttcgc
     2101 tgtacgcatg ccccgtctca tgctcccaca gcatctcccc cgtctccggg ttctccaccg
     2161 tctgccgttc gcccgtcagg cggcctttat cgtcatagcc gtagtggacc gacactcggt
     2221 ggccgtcact ggtatggctg atggtggtta gccacccgtg ttcatcatac tgccactgtt
     2281 ccgccgggtc gccgttcacc gtgcgccggg tgatacggtc tgacgcatcg taatgccaga
     2341 gggtgataag cccctcatct tcactctgcg tgagttttcc ggtcaagtca taatggtaac
     2401 gttgcgtcct gccatcaaag ccacgctgct ccgtcagcct gtcgaccggg tcataccgga
     2461 acgtggactg gctgccgttc tcgttggtca gcacggtgat gcgcccggca gcgtcatacc
     2521 ccatgctgcg cgtaagaccg ccctgcgtgg tgctgacggc ctttccccac gcatcatact
     2581 gtatctcact gcggctgccg tccggggcaa cgatagcggt gaggtcgcct gcggcactgt
     2641 actcataacg ggtctcccgc ccctgcgcat ccttctgact gaccagttgc ccacgcgggt
     2701 tataactgct gtaagtgctg atgccttctt cccggtgaac ggcgatttgt tgaccgtacc
     2761 ggtcatactc ataccgcgtc gtgtaccccg agcagtccgt gaaggccagc agctgaccgt
     2821 aacggctcca tgccatctgt tttgtactgc ccgtcgcatc ctctatccct gtcggcagct
     2881 cactcgccgg gtcatcatag ctgtaccgca tcgtctcacc gctgcgcgag gtctccgccg
     2941 tcagccttcc cttctcatca tactcccggc tgctgcgaag cccgtccggg tacgtcactg
     3001 acgtcacctg ccgctggctg ttatagccat accgcaccgt cctgccgtcc ggccccgtca
     3061 ccgccgtcac cgcgcccgac gccatatgca ggctgtactc cgtccgccgc cccgccgcat
     3121 ccgtctgcgc cttcagcctc cccgcctcat catactcgct gcgggtgatg ctcccgtccg
     3181 catgctcctt cttcaccacc cgcttcaggc cgccttcgcc ctccgtgtac agcacctccc
     3241 gccggttcag gctgtccgtg atgattaccc ggcgctctcc gtactcaaag cggtagtcca
     3301 gcccctccgg gttgacctgc tccgtcaccc gaccggtatc atcataccgg tagcggctct
     3361 ccggcctccc cgcataatgg tgtgccacca tccgcccggc gtgctccgca tcataagtaa
     3421 acccgcgaac ctgcgtcccg ctgcggtcat acaccgcccg cagttctccg ctggccgtgt
     3481 acgtgtagcg cgccagcggc gcagtcggct gttcatccgg gtatgccggg tcgtgtgtca
     3541 gccacaccgc ctccagccgg ataccgttat cggcaccgta ttctgtaccg gcgggcagcg
     3601 tgtcggggaa agcagaagaa gaggaagcag aacggggacc ggcaggggaa gataaagacg
     3661 tggcgcgctg tttacggaag gcttccgccc gctgcgcctg tgtggtcagc accaggtgaa
     3721 aacagcggcc cgcgccgtcc gtcacccccg tcaccgcgcc cgccacgtca ccctccgccg
     3781 cccggtggaa agtcagcgtc cgcccgaagc cgtccaccac gcccgtcagc acccggtacc
     3841 tgccctcgct gtttaacggg ctggtcaaag cgatgaacgc cgcgccgccg gagagtgaag
     3901 aaaaactcgc ggtgctgcgc gtaatgcgaa tgctggagga caaaagcgga cgtaacaatg
     3961 aggtggtgaa gcagtatatg gcaaaacgct ggagcgaaaa attccacggc cagcgcgata
     4021 tccaggcaca actgatgtcc catcttgact acgcgctggc tcatactgac tggcatgcag
     4081 agcgtcaggc gggcgacggt gacgccatca gccgctggac gccatatgac aagcccgtgg
     4141 tatcagcaca gaaagaactg agcaaactgc ctgtctacca gcgggtttac cagagcctga
     4201 aaacgcgggc gctgggcgtt cttcctgccg acctcaatct gcgtgaccag gtagggccaa
     4261 cctttgacca ggtgtttaca tctgccgatg acaacaaact ggttgttcca cagtttctta
     4321 cccgttacgg cctgcaaagc tattttgtaa aacagcgcga tgaactggtt gaactgacgg
     4381 cgatggattc ctgggtactt aaccttaccc gcagcgtgaa atacagtgac gccgaccgcg
     4441 cggaaatcca gcgccagttg accgagcagt atatcagcga ctacaccgcc acctggcggg
     4501 ccgggatgga caatctgaat atccgcaatt ttgagtccat cggacaactg accggggcgc
     4561 tggagcaggt tatcagcggc gaccagcctt tgcagcgggc gctgaccgtc ctgcgtgaca
     4621 acacacagcc aggcgtcttt tctgaaaaac tctctgccaa agaacgggag gaagccctgg
     4681 cagagccgga ttaccagtta ctcacccgcc tcgggcatga attcgccccg gaaaacagta
     4741 ccctggcagt acagaaagac aaagaaagca cgatgcaggc cgtgtatcag caactcaccg
     4801 agttgcaccg ctacctgctg gcaatccaga acgcgcctgt accagggaaa tcggcgctga
     4861 aagccgtgca gttacggctt gatcagaaca gcagcgatcc gatattcgcc acccgccaga
     4921 tggcaaaaac gctgcctgct ccgctcaacc gctgggttgg cagactgact gaccaggcct
     4981 ggcatgtggt gatggtggag gctgttcatt atatggaagt ggactggcgc gacagcgtgg
     5041 tgaaaccgtt taacgagcaa ctggcaaata actatccgtt taatccgcgt tctgcacagg
     5101 atgcctcact ggatgccttc gaacgcttct ttaaaccgga tggcatactg gataccttct
     5161 accagcagaa cctgaagctg tttatcgata atgacctgag tctggaggat ggcgataaca
     5221 acgtcattat tcgcgaagat attattgcgc aactggaaac tgcgcagaaa atccgtgaca
     5281 tcttcttcag caaacagaac ggtctgggaa catcctttgc cgtggaaacg gtatcgcttt
     5341 caggcaataa acgccgcagt gtactgaacc ttgacggtca gttagtcgat tacagccagg
     5401 gccgtaacta taccgcccat ctggtctggc ctaacaacat gcgcgaaggc aacgaaagta
     5461 agctgacgct catcggcacc agcggcaacg cgccgcgcag tatcagcttc agcgggccgt
     5521 gggcgcagtt ccgcctgttc ggggccggac aactgaccgg agtacaggat ggcaacttta
     5581 ccgtgcgctt tagcgtggac ggtggcgcga tgacctaccg tgtgcatacc gacacggaag
     5641 ataacccgtt cagcggtggg ttgttcagcc agtttggtct gtcagacaca ctgtactgag
     5701 gaggcaggga tgaatagtaa cgtactgaca caaactatcg ttaccggcag tgacccgcgc
     5761 gggctgccgg aattcagcgc catccgcgag gaaataaaca aagccagcca cccgtcacag
     5821 cctgagctga actggaaact ggtggagtcg ctggcgctgg cgatttttaa agccaacggt
     5881 gtggatttac ataccgccac ctactatacg cttgcccgga cacggacaca gggactggcg
     5941 ggattctgcg aaggtgcgga actgctggcg gcaatggtaa gccacgactg ggataagttc
     6001 tggccgcagg gcggcccggc gcgtactgaa atgctggact ggtttaactc ccgaaccggc
     6061 aatattctgc gtcagcaaat ctcctttgcg gaatccgacc taccactgat ctaccgcaca
     6121 gagcgggcat tgcagcttat ctgcgataag ctccagcagg tggaactgaa gcgcgttccg
     6181 cgcgtggaga atctgctcta ttttatgcag aacacgcgta aacggcttga accccagctg
     6241 aagagtaaca ctgagaacgc cgcacagacc acggtcagaa cgctgattta tgccccggaa
     6301 acacaggcat cttccacacc agaagcggta gtgcctcccc tgcccggcct gcctgagatg
     6361 aaagtggaag tgcgcagtct gacagagaat cccccacagg ccagtgtgat aaagcaaggc
     6421 agtacggtaa gagggtttat cgcagggatc gcctgttcag tggctgtcgc ctcagcattg
     6481 tggtggtggc aggtctatcc ggtgcagcag caactgttac aggttaacga caccgctcag
     6541 ggcgcagcaa cggtgtggat ggcctcacct gaactcgaaa actatgagcg caggctgcaa
     6601 caacttcttg atacctcccc ggtacagccg ctggaaaccg ggatgcagat gatgcgtgtt
     6661 gccgacagtc gctggccgga aagcctgcaa cagcaacagg cctcgacaca atggaatgag
     6721 gcactcaaaa cccgcgcaca gagtagcccg cagttgcgtg gctggttgca gacccgccag
     6781 gacttacatg cttttgcaga tctagtgatg cagcgcgaga aagagggact aaccctttcc
     6841 tatatcaaaa atgtcatctg gcaggcggag cggggactgg ggcaggaaac acccgttgag
     6901 tctctgttga cgcagtacca tgatgcccgt gcgcagaagc agaatacaga tgcgctggaa
     6961 aaacaaatta atgagcgact cgaaggcgtg ttaagccgct ggctgctgct gaagaataac
     7021 gtcatgccag aggcggcaac cggtaccacg gccgaaaaat aattatttaa ttaaaggaga
     7081 ttatcatggc aaatataagt tatttatctt tgagtggaga gacacaaggg ctaatttcag
     7141 caggttgctc aacgcttgat tctgtcggaa acaaagcgca gccagaacat aaagatcaga
     7201 tcatggttta tgcgttaatg cattccatct caagaagtca aaatgtcaat catcatgaat
     7261 taattatcac caaaccggtt gataaatcct caccattgct ggcaaaagca cttagtgaca
     7321 atgaaaaaat ggcaatatgt gaatttatac tctaccgaac cagtaaagcg ggtatttacc
     7381 agccttatta taaaataaat ctgtcaaaag cccgaatatc aagtattgat tttgtcacac
     7441 cgcatgctgt cctggaaaaa gaactggaac cacaggagag aattgccttt atttatgaag
     7501 atatttcctg ggagcatacg ttagccggta cgaatgcaat gagtaaatgg caggatcgtg
     7561 tacagtaact tactgtaata acataataaa ttactattgc ggagagataa aatggctggt
     7621 ttaaggaata acaacaacac acagaatgca cagtgggctg attatgtggg tgacattctt
     7681 cggggggctc agccgataaa ccagctagta cctcagcacc cttatcttaa cgacgtccca
     7741 ctcattgatg agttaaggca tcagaacact catcatgtta ttctcttgac acttgatgtt
     7801 gcaaaaaaaa tactcagccc aataacatca tttgattaca ttcattttat cactacacat
     7861 ccttcaggta taaaagatac actcgcctgg ttagttaacg cgggtaaatt aatgactgag
     7921 tttgatgaca atgggaaaat tatatttaac cttaatgcct taaagtatac aaaagcaagt
     7981 tactttgaaa tacttggaga gaaatatata aaaatcacaa catccagccc ctggctttta
     8041 gaaaagctcg gtaaatatat tttctcttca cgagcgcctc aagttctgga actcgctatt
     8101 ggctggcgag gagctttatc tgaatcaata aaaggagtta agttctgtat ctggttttca
     8161 gtagcctgga gaaccataga atttattatg agttcagagc gagatctggt taattttctt
     8221 ggcgattttt caatggatgt ggccaaagca gttatagccg gtggtgttgc aaccgctatt
     8281 ggaagtctgg cttcttttgc ctgtgttagc tttggctttc cagtaattct tgtcggagga
     8341 gcaattttac tgacagggat agtgtgtaca gttgttttaa atgaaatcga tgctcaatgc
     8401 catttatcag aaaaattaaa atatgcaatt agagatggac taaaacggca acaggaactt
     8461 gataaatgga aaagggaaaa catgactcca tttatgtatg ttcttaacac tccacccgtg
     8521 atatgaggat taaccatgcg tttacatgtt aaattaaaag agtttctttc gatgtttttt
     8581 atggccatat tatttttccc tgcattcaat gcttctttgt tttttaccgg agttaaacct
     8641 ctatattcaa taataaaatg ctccacggaa atattttatg attggagaat gttgattctg
     8701 tgcttcggtt
//
LOCUS       DAJOSY010000148         8652 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJOSY010000148 DAJOSY010000000
VERSION     DAJOSY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.148"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(107..727)
                     /gene="entD"
                     /locus_tag="OHN27_003938"
     CDS             complement(107..727)
                     /gene="entD"
                     /locus_tag="OHN27_003938"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCQ2288293.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(902..3142)
                     /gene="fepA"
                     /locus_tag="OHN27_003939"
     CDS             complement(902..3142)
                     /gene="fepA"
                     /locus_tag="OHN27_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCQ2288294.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3385..4587
                     /gene="fes"
                     /locus_tag="OHN27_003940"
     CDS             3385..4587
                     /gene="fes"
                     /locus_tag="OHN27_003940"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCQ2288295.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWKTQLNANWRGSYCFIPTERDDIF
                     SVPSPDRLELREGWRKLLPQAIADPLNLQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKKSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4590..4808
                     /gene="ybdZ"
                     /locus_tag="OHN27_003941"
     CDS             4590..4808
                     /gene="ybdZ"
                     /locus_tag="OHN27_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCQ2288296.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCVLPAGWDIVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            4805..>8652
                     /gene="entF"
                     /locus_tag="OHN27_003942"
     CDS             4805..>8652
                     /gene="entF"
                     /locus_tag="OHN27_003942"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCQ2288297.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQLNATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGTFEK"
BASE COUNT         1867 a         2338 c         2379 g         2068 t
ORIGIN      
        1 caatccccgc atatccggtt gtcaggtcag gatggtaagg cacctgcttt acactttcgc
       61 ccgtggtcag tgatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc
      121 acagcgttat gactatcttt tcttttatct gccagtgcac agcaaacatc tcattctcac
      181 gatgaatgat gacctgctgt ttattccagc taattatctg atagtccaga aaacctgcat
      241 cagtttggat ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgcc agtgtcagcg
      301 ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg ttccgctggt gtaataatgt
      361 tgtctgtcaa ttctcttgcg gtttgtacag aaaaaatttc ttctatatca atgccaatcg
      421 gttgacgaga taccacggct aatgccgtag tcccacagtg gctaatactg ccgtatacct
      481 ccgcaggcca gacaggttgg cgtagctcgc cgattgcggg cacacattta tagccatatt
      541 cccgcaaagc ataaacagca gcgatccgtc cggctaaatg ctctgtttta cgtttacgtc
      601 cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa atcctgctca caaaaattcg
      661 ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag
      721 ttttcatatc gaccatattc gagactgatg acaaacgcaa aactgcctga tgcgctacgc
      781 ttatcaggcc tacatggtcc tgcaatatat tgaattggca agatttttgt aggccggata
      841 aggcgttcac gccgcatccg gcatgaacga cgcgcacttt gtcaacaatc tgacgttagc
      901 atcagaagtg ggtgtttacg ctcatatacc acgtacgtcc cggctcgtta taggtatacg
      961 ccccggcacc ggcgatatag ttggcccctg ccaaatcgcc cgtggtctgg gcattacccg
     1021 cacgccacaa acgtttgtcg aacagattgt ccacgccgcc ggtcagactg acattcttcg
     1081 tcacatccca ggtcgcgctc aggccaacaa tgctgtacgg actgatttct ttggtttccg
     1141 gaccaaccgc tggctgacct ttatagttgt acttcttcgg ctgctgcttg ccgtaccagg
     1201 tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt gagttcaacg
     1261 tatactccgg gatgatcgac aaacggtcgc ccgtggtttt gttttcactc ttcagcatat
     1321 aagtgatgtt attggtccac atcaccgttt cgctaaccgg tacgtttaac gatccttcca
     1381 gaccttcaac caccgctttc ggcacgttat cccactgata gagatcggtg ccgactgcgt
     1441 tttgccctac agccacatag cccgcttcaa tcttattgcg ataatcgtta cggaaccagg
     1501 tgacgcccgc cagccacccg tcgcgtttga actccagacc aatctctttg ttgatgctgg
     1561 tttctgcttt caggtcatcg ttaccttgca gatagcagcc gcccgcgctg gcatagcaac
     1621 cctgaccttt actgtagaga atgtagttcg ggttagtctg gtacaggctc ggcgctttat
     1681 aagcacgggc gatgcccatt ttcagcgtga agtcatcgcc taaaccttgc gatatgttca
     1741 gcgccgggct ccagttattg ccgacaatac tgtgatgatc gaaacgcagc cccggcgtta
     1801 cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa cagcgagaaa atttctgctt
     1861 ttgaatacgg gctacggtcg gtggcactca cgccatcaat agcgccaccg gtattcgttc
     1921 cggtcagtgc ctgggtgttg gaacttaagt ccttcatccg ttgctgattc cactccgtac
     1981 ccagcgtcag cgtctggtta acgaggaaat caatcggcag gttaacttcg ctgtgcagca
     2041 tcacgtcatc aagatcgata tcgacgaaat cctgtgtcgc tttttcgtta aatttccctt
     2101 cggtaccgcc cgccagacct tccggaatac gcgagttacg ggtgtgttcg tactgcaccc
     2161 agttgctggt ggtcacgccg ttatcccagc caccgttcca ggtcagcgcg tagttctggc
     2221 gatacagacg gttggtttca tcgccatatt tcgagcgggt ataggaatcg gagttggtat
     2281 tctgggtgtc gcccgcatac aggttaccct ggcggctgta acctgcttcc agttccagcg
     2341 attgcaatgg cgcgaaatcc cagcgcacca cgccattaat atctttgttg attacccctt
     2401 cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg cgcggactga tggccctggt
     2461 tgatatccca cgcgtcagcc tgggttttgt cgaggttgcc atacaaacgg aagctgaatt
     2521 cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt ggtggcacct tcctctttat
     2581 gttctggcgc attgaaatat gcgtcccagg agccgtgcca ctcgccgctg ccttttttgg
     2641 taatgatgtt aaccacgccg cccgccgcgc cgttgccata acgcgcagct gccggaccac
     2701 gcagaacttc aatacgttca atcatttcag gtggcaccca ggaagtatca ccacgggtat
     2761 cgcgctcgcc acgccagccc tgacgcaccg agttacggct gcttaccggc ttgccgtcaa
     2821 tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc aatctgtcgg ttattcccac
     2881 gctgaccact ggtggagtta ccggtcaggt taacgcctgg catggtacgg atgatctccg
     2941 acacatcgcg ggcaaccggg tttttgcgga tttcatctgc ggtgatggtc gaaacgccag
     3001 gcgcctgtaa gttctgctcg gcggcggtaa cgacaatagt atcgtcatgt gaaacaggag
     3061 tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc cagattgacc aacaaggcca
     3121 gggaatgaat cttcttgttc attgttttat tcctgcattt ttgccacgaa ttgcaactgt
     3181 cgggcatggt cgtcatcaac acgacgcatc ccgctaccgc gaaaaccttt gatcctgaaa
     3241 gacacgcagt gcagttggtt aattaatgtc cgcgcttccc acggcgcgcc attacgctat
     3301 tgcaaatgca aatagttatc aataatatta tcaatatatt tctgcaatca atgaaaaatt
     3361 gcatagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga gagctggtgg
     3421 cagtcgaaac atggcccgga atggcagcgt ctgaatgacg aaatgtttga ggtcactttc
     3481 tggtggcgtg atccccaagg ttctgaagaa tactcgacga taaagcgcgt atgggtctac
     3541 atcactggtg tgaccgatca ccatcagaac agccagcccc agtcgatgca gcgaattgca
     3601 ggcactaacg tctggcagtg gaagacacaa ctcaatgcca actggcgcgg cagctactgc
     3661 tttattccca ccgaacgcga tgacattttt tctgtaccat cccccgatcg cctcgaattg
     3721 cgcgaaggct ggcgaaaact attaccccag gcgatagccg atccgctgaa cctacaaagc
     3781 tggaaaggcg ggcgagggca cgctgtttct gcactcgaaa tgccgcaagc gcctctgcaa
     3841 ccgggatggg attgtccgca agcgccagaa atacctgcca aagaaattat ctggaaaagt
     3901 gaacggttga aaaagtcacg gcgtgtatgg atttttacca ccggcgatgc aacagcagaa
     3961 gaacgcccgc tggcagtttt gctcgatggc gaattttggg cgcaaagtat gcccgtctgg
     4021 ccagtgctga cttcgctgac ccatcgtcag caacttcctc ccgccgtgta tgtgttgatc
     4081 gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga tttctggctc
     4141 gcagtacagc aagagttatt acccctggtg aaagctattg ccccttttag cgatcgtgcc
     4201 gatcgcaccg tggttgccgg gcagagtttt ggtgggcttt ccgcgctgta tgccggactg
     4261 cactggcctg aacgctttgg ctgtgtatta agccagtcag gatcgtactg gtggccgcat
     4321 cggggcgggc agcaagaggg cgtgttactt gaaaagctaa aagctggtga agttagcgcc
     4381 gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc cgatgatcat gcgggccaat
     4441 caggcgctgt atgcgcaatt acaccccata aaagaatcca ttttctggcg tcaggttgac
     4501 ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggctaat cgacctctgg
     4561 caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc gatgatccgc
     4621 agggagcgtt ttacatattg cgcaatgcgc aggggcaatt cagtctgtgg ccgcaacaat
     4681 gcgtcttacc ggcaggctgg gacattgtgt gtcagccgca gtcacaggcg tcctgccagc
     4741 agtggctgga agcccactgg cgtactctga caccgacgaa ttttacccag ttgcaggagg
     4801 cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga tggcagaaaa
     4861 actgtcagaa ttaccctccg cctggagcgt ggcgcattac gttgagttaa ccggagaggt
     4921 tgattcgcca ttactggccc gcgcggtggt tgccggacta gcgcaagcag atacgctgcg
     4981 gatgcgtttt acggaagata acggcgaagt ctggcagtgg gtcgatgatg cgctgacgtt
     5041 cgaactgcca gaaattatcg acctgcgaac caacattgat ccgcacggta ctgcgcaggc
     5101 attaatgcag gccgatttgc agcaagatct gcgcgtcgat agcggtaaac cactggtctt
     5161 tcatcagctc attcaggtgg cagataaccg ctggtactgg tatcagcgtt atcaccattt
     5221 gctggtcgat ggctttagtt tcccggccat tacccgccag atcgccaata tttactgcac
     5281 atggctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg atgtagtgga
     5341 agagtaccag caataccgcg aaagcgaagc ctggcagcgt gatgcggcat tctgggcgga
     5401 acagcgtcgt caactgccac cgcccgcgtc actttctccg gcacctttac cggggcgcag
     5461 cgcctcggca gatattctgc gcctgaaact ggaatttacc gacggggaat tccgccagct
     5521 ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc cttgcgctgg cggctttgtg
     5581 gctggggcga ttgtgcaacc gcatggacta cgctgccgga tttatcttta tgcgtcgact
     5641 gggctcggcg gcgttgacgg ctaccggacc cgtgctcaac gttttgccgt tgggtattca
     5701 tattgcggcg caagaaacgc tgccggaact ggcaacccga ctggcggctc aactgaaaaa
     5761 aatgcgtcgt catcaacgtt acgatgccga acaaattgtc cgtgacagcg ggcgagcggc
     5821 aggtgatgaa ccgctgtttg gtccggtact caatatcaag gtatttgatt accaactgga
     5881 tattcctggt gttcaggcgc aaacccatac cctggcaacc ggtccggtta atgaccttga
     5941 actggccctg ttcccggatg aacacggtga tttgagtatt gagatcctcg ccaataaaca
     6001 gcgttacgat gagccaacgt taatccagca tgctgaacgc ctgaaaatgc tgattgccca
     6061 gttcgccgcg gatccggcgc tgttgtgcgg cgatgtcgat attatgctgc caggtgagta
     6121 tgcgcagctg gcgcagctca acgccactca ggttgagatt ccagaaacca cgcttagcgc
     6181 gctggtggca gaacaagcgg caaaaacacc ggatgctccg gcgctggcag atgcgcgtta
     6241 cctgttcagc tatcgggaaa tgcgcgagca ggtggtggcg ctggcgaatc tgctgcgtga
     6301 gcgcggcgtt aaaccagggg acagcgtggc ggtggcacta ccgcgctcgg tctttttgac
     6361 cctggcactc catgcgatag ttgaagctgg agcggcctgg ctaccgctgg ataccggcta
     6421 tccggacgat cgcctgaaaa tgatgctgga agatgcgcgt ccgtcgctgt taattaccac
     6481 cgacgatcaa ctgccgcgct ttagcgatgt tcccaattta acaagccttt gctataacgc
     6541 cccgcttaca ccgcagggca gtgcgccgct gcaactttca caaccgcatc acacggctta
     6601 tatcatcttt acctctggct ccaccggcag gccgaaaggg gtaatggtcg ggcagacggc
     6661 tatcgtcaac cgcctgcttt ggatgcaaaa tcattatccg cttacaggcg aagatgtcgt
     6721 tgcccaaaaa acgccgtgca gttttgatgt ctcggtgtgg gagtttttct ggccgtttat
     6781 cgcaggggca aaactggtga tggctgaacc ggaagcgcac cgcgacccgc tcgctatgca
     6841 gcaattcttt gccgaatatg gcgtaacgac cacgcacttt gtgccgtcga tgctggcggc
     6901 atttgttgcc tcgctgacgc cgcaaaccgc tcgccagagt tgcgcgacgt tgaaacaggt
     6961 tttctgtagt ggtgaggcct taccggctga tttatgccgc gaatggcaac agttaactgg
     7021 cgcgccgttg cataatctat atggcccgac ggaagcggcg gtagatgtca gctggtatcc
     7081 ggcttttggc gaggaactgg cacaggtgcg cggcagcagt gtgccgattg gttatccggt
     7141 atggaatacg ggtctgcgta ttcttgatgc gatgatgcat ccggtgccgc cgggtgtggc
     7201 gggtgatctc tatctcactg gcattcaact ggcgcagggc tatctcggac gccccgatct
     7261 gaccgccagc cgctttattg ccgatccttt tgccccaggt gaacggatgt accgtaccgg
     7321 agacgttgcc cgctggctgg ataacggcgc ggtggagtac ctcgggcgca gtgatgatca
     7381 gctaaaaatt cgcgggcagc gtatcgaact gggcgaaatc gatcgcgtga tgcaggcgct
     7441 gccggatgtc gaacaagccg ttacccacgc ctgtgtgatt aaccaggcgg ctgccaccgg
     7501 tggtgatgcg cgtcaattgg tgggctatct ggtgtcgcaa tcgggcctgc cgttggatac
     7561 cagcgcattg caggcgcagc ttcgtgaaac attgccacca catatggtac cggtggttct
     7621 gctgcaactt ccacagttac cacttagcgc caacggcaag ctggatcgca aagccttacc
     7681 gttgcctgaa ctgaaggcac aagcgccagg gcgtgcgccg aaagcgggca gtgaaacgat
     7741 tatcgccgcg gcattctcgt cgttgctggg gtgtgacgtg caggatgccg atgctgattt
     7801 cttcgcgctt ggcggtcatt cgctactggc aatgaaactg gcagcgcagt taagtcggca
     7861 ggttgcccgc caggtgacgc cggggcaagt gatggtcgcg tcaactgtcg ccaaactggc
     7921 aacgattatt gatgctgaag aagacagcac ccggcgtatg ggattcgaaa ccattctgcc
     7981 gttgcgtgaa ggtaatggcc cgacgctgtt ttgtttccat cctgcgtccg gttttgcctg
     8041 gcagttcagc gtgctctcgc gttatctcga tccacaatgg tcgattatcg gcattcagtc
     8101 accgcgcccc aatggcccca tgcagacggc ggcaaacctg gatgaagtct gcgaagcgca
     8161 tctggcaacg ttacttgaac aacaaccgca tggcccttat tacctgctgg ggtattccct
     8221 tggcggtacg ctggcgcagg gtattgcggc gcgactgcgt gcccgtggcg aacaggtggc
     8281 atttcttggc ttgctggata cctggccgcc agaaacgcaa aactggcagg aaaaagaagc
     8341 taatggtctg gacccggaag tgctggcgga gattaaccgc gaacgcgagg ccttcctggc
     8401 agcacagcag ggaagtactt caacggagtt gtttaccacc attgaaggca actacgctga
     8461 tgctgtgcgc ctgctgacga ctgctcatag cgtaccgttt gacggtaaag cgacgctgtt
     8521 tgttgctgaa cgcacacttc aggaaggtat gagtcccgaa cgcgcctggt cgccgtggat
     8581 agcggagctg gatatctatc gtcaggattg tgcgcatgtg gatattatct ctccagggac
     8641 gtttgaaaaa at
//
LOCUS       DAJOSY010000149         8551 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJOSY010000149 DAJOSY010000000
VERSION     DAJOSY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.149"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            23..673
                     /locus_tag="OHN27_003943"
     CDS             23..673
                     /locus_tag="OHN27_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HCQ2288298.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKDLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(714..1208)
                     /locus_tag="OHN27_003944"
     CDS             complement(714..1208)
                     /locus_tag="OHN27_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288299.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     GLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(1423..3063)
                     /gene="pgm"
                     /locus_tag="OHN27_003945"
     CDS             complement(1423..3063)
                     /gene="pgm"
                     /locus_tag="OHN27_003945"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCQ2288300.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(3089..3634)
                     /gene="seqA"
                     /locus_tag="OHN27_003946"
     CDS             complement(3089..3634)
                     /gene="seqA"
                     /locus_tag="OHN27_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCQ2288301.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            3819..4583
                     /gene="ybfF"
                     /locus_tag="OHN27_003947"
     CDS             3819..4583
                     /gene="ybfF"
                     /locus_tag="OHN27_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCQ2288302.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMSQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            4723..5016
                     /gene="ybfE"
                     /locus_tag="OHN27_003948"
     CDS             4723..5016
                     /gene="ybfE"
                     /locus_tag="OHN27_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCQ2288303.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            5156..5686
                     /gene="fldA"
                     /locus_tag="OHN27_003949"
     CDS             5156..5686
                     /gene="fldA"
                     /locus_tag="OHN27_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCQ2288304.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            5896..5982
                     /gene="uof"
                     /locus_tag="OHN27_003950"
     CDS             5896..5982
                     /gene="uof"
                     /locus_tag="OHN27_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCQ2288305.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            5975..6421
                     /gene="fur"
                     /locus_tag="OHN27_003951"
     CDS             5975..6421
                     /gene="fur"
                     /locus_tag="OHN27_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCQ2288306.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(6505..6831)
                     /locus_tag="OHN27_003952"
     CDS             complement(6505..6831)
                     /locus_tag="OHN27_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288307.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(6881..8287)
                     /gene="chiP"
                     /locus_tag="OHN27_003953"
     CDS             complement(6881..8287)
                     /gene="chiP"
                     /locus_tag="OHN27_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCQ2288308.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2116 a         2132 c         2110 g         2193 t
ORIGIN      
        1 agcacacaag gagcaagtag cgatgcgtga aatgaatcag ttagtaaata ctgaagattc
       61 agcctggcca ataatccaga actggttaaa agacgccaca aaccataccg aactcttacc
      121 ggttaataaa gatttagccg aaaccgcact ttatcaatta caggtgacaa caaaatcacc
      181 aatgggtgcg cttgtctatg gctccggagg tctgctgatt gataatggtt ggttacgcat
      241 agcaggatct ggtcaccccc ggttgccacg agatccagta agttggacac aacgccctga
      301 attcgcaggc gtgcgggctt tacccatcgc tgacgatgtc gcagggggaa tttttgctct
      361 taatggtggc gatcttgggg aagatactgg ctgcgtttat tattttgcgc cagatacgct
      421 gaattgggaa agtctggaag ttggatattc tgagtttctt cagtgggcat tatccggtga
      481 tttagatacc ttctatgaaa atgtacgatg gcagcaatgg cgagaagacg taataaaatt
      541 atcggcaacg gaagccttca cgttctaccc tttcttatgg gtacaaagtg aagaagcaag
      601 aacaagaaaa gttatttcgc ttactgaact ctgggaaatg cagtatcaaa tgaaagaaac
      661 attcacgcaa taagacatgt tcaggtccgg gtttcatttc cggactcact attttaatgt
      721 gtatttatcg ctttcaattc ttgctggcaa ttttctgact ggcagcgtcg taactcaata
      781 atcccgacat gcatcatcga gccaaaaaat ccgctttcgc tgacataatc ctcattgact
      841 atcggtcgag tgccgggctt gccctggcaa acccgcataa aaaatgaggt gtagcattgc
      901 caaccgggat ttaaaccgac cttttcttgt gctgacatat tatgaagata agtaagttct
      961 ttttcgttcc cgcctgacaa ctgccagcgg cgggtattat caggtaagtt atcaccacgg
     1021 caatttgtct tacaccggga aggtgcaata tttaccgcaa tgctacccgt taatcctctg
     1081 gttttatctt taggggtcag taaccagtcg ccagctaatg atgattgttc aggttgggcg
     1141 catgcagtca gcagtaatgt ggcggtaatt agtgacacta tgacgaccag actcctgttg
     1201 gtcttcattg ggatttcctt gagctactca gtttgttgac aaagtgttcg ttgtttttgc
     1261 cggatgcggc gtgaacgcct tatccggcct acaaaatcgc gtaaattcaa tatattgcac
     1321 catcctcgta ggcctgataa gcgtagcgca tcaggcaatt ctgtgtttgt catacgtaaa
     1381 aaagggcgat cttccgaccg ccctttttct tatttaatgt gtttacgcgt ttttcagaac
     1441 ttcgctaaca atctcaaccg cttctttctc gatctgcttg cgatgttctt caccgaggaa
     1501 gctttcgcag tagatcttat atgcgtcttc cgtgcctgac ggacgcgcgg cgaaccagcc
     1561 gttgtcagtc atcactttca gaccgccaat agaagcaccg ttgcccggag cagcagtcag
     1621 gcgcgcggtg atcgggtcac ctgccagggt gctggcgctc accatttccg gagacagctt
     1681 agacagcgcc gctttttgtg cggaagtcgc agctgcctgc aaacggttgt agctcggcgc
     1741 accaaagcgt tttgccagtt cgttgtagtg ttcctgcggg ttcttaccgg tgacagcggt
     1801 gatttccgcc gccagcagac acatgatgat gccgtctttg tcggtggacc acggcgtgcc
     1861 gtcgaaacgc aggaaggaag cccctgcgct ctcttcgccg ccaaagccga agctgccgtc
     1921 gaacagacca tcaacaaacc atttgaaacc taccgggact tctaccagct tacgacccaa
     1981 gtcattgacc acacggtcga tcatcgcaga agaaaccagc gttttaccaa cggcaacatc
     2041 tttgccccac tgcggacgat gctggaacag gtaattgatc gccaccgcca ggtagtggtt
     2101 cggattcatc aaacctgccg gagtgacgat accgtgacgg tcataatccg ggtcgttagc
     2161 aaacgccaga tcgaacttat cacgcagtgc cagcaggccc gccatcgcac actcggagga
     2221 gcagtccata cggatcgcgc cgtctttatc aaggtgcata aagcggaagg tttgatcgac
     2281 ctgatcgtta acgatagtca ggttgaggtt gtaatactcg ccaatacgct tccagtattc
     2341 gataccggaa ccgcccagcg gatcaacgcc aagcgtcagg cccgctttct gaatcgccgc
     2401 catatcaacg atatcggcca gcccttccac gaacggctgc accagatcct gctctttcac
     2461 atgaccggat gccatcgctt cgtcgaggga gatacgcttc acgcctttca ggccatcggc
     2521 cagcagtgcg ttggccctgt cttccaccac tttggtgacg ttggtatcag ccgggccacc
     2581 atttggcgga ttgtacttga taccaccatc ttccggcggg ttatgggacg gtgtaatcac
     2641 gataccgtct gccagcgggc cacctttttt attgtgaacc aggatggcat tggaaacggc
     2701 aggcgttgga gtgaagccat tgttttcctg cacaatgaca tcaacgccgt tcgctgccag
     2761 cacttccagt acggaaatga acgcaggctc ggacagggcg tgagtatctt tacccacata
     2821 gcaagggcca gtgatgccgt ttttcgcacg ttcttcagca attgcctgag cgatcgccag
     2881 aatgtgcggc tcgttaaagc tgtggcgcgc tgcactgcca cggtgaccgg aagtaccgaa
     2941 tttcaccgcg tgctccgcat tccctgcttc tggtttcagt acataatatt gcgccgtcag
     3001 ttgggcgacg ttaatcaaat cactctgttg tgcaggttgg cctgcacgat tgtggattgc
     3061 cattgctttg tcctttgtct gcaacgtttt agatagttcc gcaaaccttc tcaatcaatt
     3121 ccgccgggaa ttgcatcgac tgcatgatgt gttcgatcat gctgcattta cggccggtgt
     3181 tggtgttggt gatcacccaa tacggcgtgc ctggcacatg tttcggcttg gtctgattac
     3241 catttttcag cagcgtttgt tcatctgccg caaagtaaac gcgtgtacga ccgtgcaacg
     3301 attccgttgc ttcggcaaac gcctgggcgt caagagaata tagtgtagac aacagcagca
     3361 taaagcgatt gaccgctcgc ttttgctctg cgtattcgtc cgaaagcaga agttcacgca
     3421 ttgcgcgaac cttgtcttta atcgttttga ccggcttcgc ttcgacgata gcaggtgacg
     3481 caacgcgaac ctctttcgtc accggagcag caggttgtga tgcggcggaa aatttcaaca
     3541 tacgccgtaa aatgtcggat gcgctctcgc cgatatgctt agtgtggctg gcaatatagc
     3601 tgtagagttc atcatcaact tcaatcgttt tcatcttaat ccagtgcagt gtcttagctg
     3661 aatacaagtc gccaggagta tatgaataaa tcccggcagc ggatagcgtc aagcctggct
     3721 tgcagaaaaa ctcggaataa accttatttg ttgcagccct gtggcacaat ggtcaacata
     3781 ataccctaac ccgacaacgc gaaaaaaaga actttgccat gaaattgaat atccgcgcgc
     3841 aaactgcaca aaaccagcac aataattctc ccatcgttct tgtccatggt ctgtttggta
     3901 gcctcgacaa ccttggcgta ctggctcgcg atctggtaaa cgatcacaat atcatccagg
     3961 ttgatatgcg taaccacggt ctttcaccga gagagccggt aatgaattac ccggcgatgg
     4021 cgcaggatct tgttgatacc ctggatgcac tgcagatcga caaagcaaca tttatcggtc
     4081 actccatggg cggtaaagcg gtaatggcac ttactgcact ggcccccgat cgcatcgata
     4141 aactggtggc gatcgatatc gcaccggtcg actatcacgt acgccgtcat gatgagattt
     4201 tcgcagctat caacgcggtc agtgaatcgg acgcacaaac tcgccagcaa gcagcagcaa
     4261 taatgagcca gcatcttaat gaagaagggg tgattcagtt tctgttgaaa tcttttgttg
     4321 acggggagtg gcgctttaac gtgccggtat tgtgggatca gtatccgcat attgtgggtt
     4381 gggagaaaat cccggcatgg gatcaccctg ccctgtttat ccctggtggt aattctccgt
     4441 atgttagcga gcagtaccgt gatgatttac tggcacaatt tccacaggca cgggcgcatg
     4501 tgattgcagg cgcgggtcac tgggtccatg ctgaaaaacc ggatgcggta ttacgcgcta
     4561 tccgtcgcta tctcaatgat taacgcaact gattaaaaac ccagcgtccg cgtcgctttc
     4621 cgcgcgtggg cgttggcgtg actcatcgcg ctgatgtatt atggcgcgct atccatccgg
     4681 gcagaggcct ggctgattgt ttcccccgaa gtcaccaaga tcatggccaa agaacaaacg
     4741 gaccgtacga cattagatct gttcgcgcac gagcgtcgac cgggacgacc gaaaactaat
     4801 ccgctttcgc gcgatgaaca gctgcgtatt aataaacgca accagctaaa acgcgacaaa
     4861 gtacgtggcc ttaagcgtgt cgaactgaag ctgaacgcgg aagctgtcga ggcgctgaac
     4921 gagctggcgg aatcgcgcaa tatgagccgt agcgaactga tcgaagagat gttaatgcag
     4981 caactggcgg cgctgcgtag tcagggcatc gtttaaattt ccactttcat gtagcacagt
     5041 gtgcagtcct gctcgtttgc tgattccgca agactgcctg ttctgctatg attgccttta
     5101 tccgtgggca attttccacc cccatttcaa taagtttcaa gaggttattt cactcatggc
     5161 tatcactggc atctttttcg gcagcgacac cggtaatacc gaaaatatcg caaaaatgat
     5221 tcaaaaacag cttggtaaag acgttgccga tgtccatgac attgcaaaaa gcagcaaaga
     5281 agatctggaa gcttatgaca ttctgctgct gggcatccca acctggtatt acggcgaagc
     5341 gcagtgtgac tgggatgact tcttcccgac tctcgaagag attgatttca atggcaaact
     5401 ggttgcgctg tttggttgtg gtgaccagga agattacgcc gaatatttct gcgacgcatt
     5461 gggcaccatc cgcgacatca ttgaaccgcg cggtgcaacc atcgttggtc actggccaac
     5521 tgcgggctat catttcgaag catcaaaagg tctggcagat gacgaccact ttgtcggtct
     5581 ggctatcgac gaagaccgtc agccggaact gaccgctgaa cgtgtagaaa aatgggttaa
     5641 acagatttct gaagagttgc atctcgacga aattctcaat gcctgatgtg atgcggcgta
     5701 gactcatgtc tacgccgtat taatagataa tgccaatcaa aataattgct acaaatttgt
     5761 aacttttgct gttgtacctg tacaatgtcc cggtgttcaa gtggccttgc cgttgtaaat
     5821 gtaagctatg ccacgttttt attaacaata tttgccaggg acttgtggtt ttcatttagg
     5881 cgtggcaatt ctataatgat acgcattatc tcaagagcaa attctgtcac ttcttctaat
     5941 gaagtgaacc gcttagtaac aggacagatt ccgcatgact gataacaata ccgccctaaa
     6001 gaaagctggc ctgaaagtaa cgcttcctcg tttaaaaatc ctggaagttc ttcaggagcc
     6061 ggacaaccat cacgtcagtg cggaagattt atacaaacgt ctgatcgata tgggtgaaga
     6121 aattggtctg gctacggtat atcgcgtact gaaccagttt gacgacgctg gtatcgtcac
     6181 ccgccacaat tttgaaggcg gtaaatccgt atttgaactg acacagcaac atcaccacga
     6241 tcacctgatc tgcctcgact gcggcaaggt tatcgaattt agtgatgatt ccatcgaagc
     6301 gcgtcagcgt gaaattgccg caaaacatgg cattcgcctg actaaccaca gtctctatct
     6361 ttacggtcac tgtgccgaag gcgattgccg cgaagatgag cacgcgcacg aaggcaaata
     6421 agccagcctg aacgagaaaa gccaacctgc gggttggctt ttttatgcaa gggaaataag
     6481 aaaagcgaga gttacagctc tcacttattt gttattactg cgaatttcct gccagacttt
     6541 atcacaatcg gctttcaccg cctgatcatt tccggtttgc gttgcacgca gacactgctg
     6601 ataatccagt acgcgtacac tttcctggtc ggcaaactgc tgatgttgtt tctctttctt
     6661 cagcacgttt aacacgctct ggcaggcttc aattttttct ggcgaacctt gtgcggtgtt
     6721 gatacaggcg ctgtatgcct ctttcagacg gctgtcttcc tgtggcgcgg ttgattgcgc
     6781 acaagccacc agccccgatg ccatcatggc gatgagaatc agttttttca tagcaaattc
     6841 ctcaaatgtg cggcaggcac aaaacctgcc gcgtcgggca tcagaagatg gtgaatggtg
     6901 cgattaccat aaatttcacg tcacgctcat cctggaagat gttgccgtaa ccaccgcccc
     6961 agcttgggat gtcggagtgg ttgtcgtatt cggtgaaatg cagtttgaac atcgtgcctt
     7021 tggcgcgacc gtcctgaatg gtgtagaccg catccaggct gtatgcagac tcttcaatag
     7081 tccggttttt gtcgtagtac gcatccggat tgctctgcca ggtcgcaggt ttagcatccc
     7141 atgcgtaaac gtaggaagcg ccgatggcga agcctggaag attccagttt ttcaggtcat
     7201 acatcgcacc gaagaagact gctttttcgc cgttggcgtt gaagtcagaa cggttgtccc
     7261 accagatatc caggcgaccg tttgaggaag cgtaggttgg agtcatacgt tgcaggaagt
     7321 atccctgctg accgtcagcc ttaacccagg tgccttcgag gcgcaaatcc actacgtcag
     7381 ccgcccggta accaaaggtc aacgcctgca gccaggcggt gccgtcataa aggtcgttga
     7441 cgctgcgatc gtcaacttta tcgcgggtac cgtagaactg gtagctggtg gttaacgggc
     7501 taccggcgat atcaaatttg tagctggctt tggcaaaata ttgatcgata tacccttccg
     7561 cctgaccaaa agccgcttcc agtacgaagt tatttttgaa gtcgtatttc gccccaaggg
     7621 agtgcagata atcaactttg gtggttttat cgttctgata aaactcatcc atttccagat
     7681 gccacggcgc tttgtattcg ttggtccaca tgtaggagaa actcaacgca ccggcatcgc
     7741 cgtaatcaaa attcgctccg gcttccgcac cctgataagt acccggcata aagctccagt
     7801 gaggcgctaa cagcgtctga ccggttggct gaatataacc tgccctcgcc caaaccggac
     7861 cgtatttaaa tttggccgct gctttataca ggcttatacc gcttttgtcg ccggaccagt
     7921 cttcgtcata ggctttatta ctttttgaaa acgcgatttc gttcgggtga gagctgtcgc
     7981 cgttttccgc catttcaatc gccgtaaacg cggcaatatc aaggccgaac atatcagcag
     8041 cataaccaga ctgaaaatcg aggttggcat tccaggtgga gtgagaaagg ttggttttgt
     8101 atttgtcgcc gtcggtaaca tctttacggt cgcgttcacg ctgccagtaa tatataccgc
     8161 cggttaaggt tgaatcgtcg atgaatcctt cggcgcgagc ctccggcatt gccataaagc
     8221 ccgacattgc tgtaacaccg gcgatagcca gcgccagcgt actacgtttg ccactaaacg
     8281 tacgcatggg ttaatcctct ttgacgtata aattgctgca ccaaaggtga acagcgaaaa
     8341 aataaaaatt aaaaaagtag cgttgagaaa agtctcgctg actacaggaa cagactattt
     8401 ttcgcgacgc gaattatcaa tctctttctg taaccagaat gtaagaacat gagcaaccgc
     8461 acatattttg atgatttttg ttacaaggct taagtttgcg gttgaaaggc ttgcagaaga
     8521 ataaaagcgt gaaattccag caagatgaaa g
//
LOCUS       DAJOSY010000150         8496 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJOSY010000150 DAJOSY010000000
VERSION     DAJOSY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.150"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(270..345)
                     /locus_tag="OHN27_003954"
     tRNA            complement(270..345)
                     /locus_tag="OHN27_003954"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(310..312),aa:Asn,seq:gtt)
     gene            complement(446..2089)
                     /gene="yeeO"
                     /locus_tag="OHN27_003955"
                     /pseudo
     CDS             complement(446..2089)
                     /gene="yeeO"
                     /locus_tag="OHN27_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            2094..2169
                     /locus_tag="OHN27_003956"
     tRNA            2094..2169
                     /locus_tag="OHN27_003956"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2127..2129,aa:Asn,seq:gtt)
     gene            complement(2207..3157)
                     /gene="cbl"
                     /locus_tag="OHN27_003957"
     CDS             complement(2207..3157)
                     /gene="cbl"
                     /locus_tag="OHN27_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCQ2288309.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTITTTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVT
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDTNTVWLGLKR
                     GQPQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3259..4176)
                     /gene="nac"
                     /locus_tag="OHN27_003958"
     CDS             complement(3259..4176)
                     /gene="nac"
                     /locus_tag="OHN27_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCQ2288310.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            4502..4577
                     /locus_tag="OHN27_003959"
     tRNA            4502..4577
                     /locus_tag="OHN27_003959"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4535..4537,aa:Asn,seq:gtt)
     gene            complement(4633..5568)
                     /gene="ldtA"
                     /locus_tag="OHN27_003960"
     CDS             complement(4633..5568)
                     /gene="ldtA"
                     /locus_tag="OHN27_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCQ2288311.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALERRSGMPVQISSGSRQMF"
     gene            complement(5630..6709)
                     /gene="cobT"
                     /locus_tag="OHN27_003961"
     CDS             complement(5630..6709)
                     /gene="cobT"
                     /locus_tag="OHN27_003961"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCQ2288312.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6721..7464)
                     /gene="cobS"
                     /locus_tag="OHN27_003962"
     CDS             complement(6721..7464)
                     /gene="cobS"
                     /locus_tag="OHN27_003962"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCQ2288313.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7461..8006)
                     /gene="cobU"
                     /locus_tag="OHN27_003963"
     CDS             complement(7461..8006)
                     /gene="cobU"
                     /locus_tag="OHN27_003963"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCQ2288314.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            8202..8474
                     /locus_tag="OHN27_003964"
                     /pseudo
     CDS             8202..8474
                     /locus_tag="OHN27_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2323 a         2304 c         1847 g         2022 t
ORIGIN      
        1 tttcccgata aagatgcgac agctacatcc cgcataaggc atttttctct ttatctttgt
       61 acggtacttc atggaacaga atttttgacc ttgtgaatcg tgatgtctgt tggggagggg
      121 caatttgctc actgaagcgt gagactcgat taagcgcacg aaacacagaa atcaaaaacc
      181 cggccactct tttacaaagt ggccgggtaa aaataatttt tattttttaa ctgtcctgag
      241 actcatagag atgtctcaaa actaaaattt ggctcctctg actggactcg aaccagtgac
      301 atacggatta acagtccgcc gttctaccga ctgaactaca gaggaatcgt gagaacgagg
      361 cgaatattag cgatgcccac ccacaatgtc aaagcctgtt ttttaaattt gaaatcgttt
      421 gctgaaataa tctgcatttt gtcgtttatt ccgacacaac tggctttttt tcacactttt
      481 gcggctcgga tcgagggtat ttccacagcc aacgtccagt aaccattcgc cagtaaaaca
      541 gcacggcccg cacagcccag tcggcaaaca ttcccatcca gacaccaacc acaccccagc
      601 caagcatgat tcccagcaca taaccgacta caacccggca accccacatg ctcaacatag
      661 aaacccacat ggcgtaacgg gcatcacgag cccccttaaa tccagcgggt agcacccatg
      721 aggcggacca aataggcata aataaagcat ttagccaaat cagaatcaca acgacgtgtt
      781 taacctgtgg atcctgggtg taaaacgatg ccataacccc ggcaaaggga gccgttagcc
      841 aggcgatggc cgttaatcca agagtggaca gccagaacac atgccgcaac tgaatctctg
      901 cttgcgctat ctaccctacc cccaaccttc ggcctgtaat gatcgtagaa gcagaaccga
      961 gcgcacttcc gggtaagttg ataagagccg caattgagaa cgcgataaaa tttccggcaa
     1021 taacgctggt ccccatcacg gcaacgaaca tttgggttaa taaccgacca ctggtaaata
     1081 acactgattc gacactcgct ggaataccaa tccccatgac ttcccagata atgctaaaat
     1141 tcagcagttt aaaatagctc tttaacgaaa tccttagcgc aggattaaaa ccaatcgcca
     1201 gcacccacaa aattgcaact gcgccaatat aacgagaaat ggttaaaccc aacccagccc
     1261 cgacaaatcc cagtcccggc caggagaaaa ggccgtaaat caatatgccg ctaataataa
     1321 tattaagaat attcaggcta ccgttaatca gtagcggtat tttcgtattc cctgcaccac
     1381 gaagtgcccc gctaccaata agagtgatgg cagctgctgg ataactgagt acggtcagct
     1441 ccagataagt caacgccagt gctttaactt ctgtcgtggc atcacccgcg acgaaatcaa
     1501 taatttgttc gccaaaatga tgaattagcg ttgccaacag tacggcaaac aacgtcatga
     1561 tcaccaatga ctgccgcgtc gccaccctcg ctcgtcgtcg atcccgctta ccgagactaa
     1621 atgccacaac gacagtagta ccaagatcga tagctgcaaa aaaagccata atgaccatat
     1681 tgaagctgtc cgccaatccc acgccggcca tcgcatcttt tcccagccag ctgaccagaa
     1741 aagtgctcag gacccccatc aacaggacac aggcgttctc catgaagata ggaacagcaa
     1801 gcggggttat ctcgcgccag aacaacactt tgtagctctt gcgtttagcg tgccagcgag
     1861 tgtcgtgaac aacctggcgt aaagcagagg agatattcaa agccgacctt aattgcagaa
     1921 agtgaaacca catttcaaat gatgagggag aatcagcaaa gctgcaaaga ttttcgccaa
     1981 caaattgtct gcaaatgcaa caaactgttg atagaaacgg caaacagttg ggaaatttaa
     2041 aaatcgggtt tgacaaaata tttttcgccg ttaagatgtg cctcaacagc gattcctctg
     2101 tagttcagtc ggtagaacgg cggactgtta atccgtatgt cactggttcg agtccagtca
     2161 gaggagccaa atttctgaaa agcccgcttt tatagcgggc tttttgctat atctgataat
     2221 caatttcctc ttcactgttt tccatcacct ggcgcttgat atcctcaact gacagtcctg
     2281 cattacaaag ttccagaaag cgccagacat agttacgctg aggttgtcct cgcttcagtc
     2341 ccaaccagac agtattagta tcgaaaagat gccgcgtatc caggcggatt aaattctctt
     2401 cctcttgttc gccgctggat tgttcggcaa ctaatccgat cccaagccca agagcaacat
     2461 aggttttaat gacatcagaa tcctgtgcac ttaatacaat atctgccggc aaacctttgc
     2521 gggcaaatgc gtcatcaatg cgtgagcgcc ccgtaatccc ctgtcggtaa gtaattaacg
     2581 gccacttcgc tattgattcc agcgtcaacg gtgtaatttg cgtcaaggga tgatcgagtg
     2641 gaacaagcaa actatggtgc caacgaaacc acgggaaggt gacgagctgc gggtcattac
     2701 tcaaacgctc gctggcgata ccaatatcag cttcgccatt ttgcaacaac gtcgcaattt
     2761 cctgtggtgt accctggatt agctccagcc gaacctccgg gaaaagttcg cgaaaagctt
     2821 taatgacctc aggcaagcta taacgtgcct gagtatgcgt cgttgtaata gtgagaacgc
     2881 cagacgtatc gttggtaaac aggtctgcga gccgacgaac attactggct tcattaagaa
     2941 tgcgttctgc aatgaccagt aatgctttgc ccggttcagt catgcccagc agtcgcttac
     3001 ctcgtcgaac aaatatttcg atgccaagtt catcctccag ttcccgaata tgacggctaa
     3061 cgcctgactg tgaggtaaaa agcatattcg caacctctgt caggttgtaa tcctgacgtg
     3121 cagcctcgcg gattatcttt agttgttgga aattcacggt aaactccggg cagttcagat
     3181 atcccgttat tgttaaagtc taatgcccgg cataacaaat aataaaaacc cgcatcttat
     3241 tccatcccga tataacgctt agctcaccaa ttgccactgc cttttttcca tcactggaga
     3301 actcatcact gacattaaca actctttcac tgcctgtgcc tgtggtgaca agtttgctct
     3361 ggcgggtaaa tttaaggaca aagagagact cattgaagga gtggtaatgc gtgacatcca
     3421 cccatttact gcgccacata acgaacgcgc agccgattca ggtaatactg caacgcccat
     3481 gccgctggca atcgctgcgg taagcgtggc aatagactca atttcaccaa tgacttttgc
     3541 cgtgagccgc cgtagggaaa aagcctcatc aacacgaagt ctaacagcgc tgtaatcact
     3601 ggggagaaaa aggttcattt gcgcaatagc attaacatca acgttttgcc ccgggcaatc
     3661 ttgagttcct accaggaaaa gatcttcttt cagcaaagcc tgactggata ccccagccac
     3721 aggggaatgc tcataaatca ccgccatatc gagttggtga tttatcagtt tttcgttaag
     3781 cactgcacca ctattttcat gaagatagat aacgacctcc gggaattcag cgcgaaccgc
     3841 ctgtaataag ggcatggtga tggatgacgc agcggttcct ggcgcaaagc caatcgagac
     3901 ttgccccgat aatgactgac caacgttatg caccgccagt tgggcctgct cacactgacg
     3961 taaaatggcc cgtgcatggg tatagagaat ttttccggcg tctgttggcg taacgccccg
     4021 ttttgtgcga atcaaaagct gttgatttaa ctcaccttcc agtgtggcaa cctgctggct
     4081 gagcgctggt tgtgcgatat gcaatacttc agcagcctgg gtcaggctac caatatctac
     4141 aatttttacg aagtatttca ggcgtctgaa gttcatgttg cctccggttt ttgagaatcg
     4201 gcccaagtgc cgccattact tacaaccaga ctgcaagatg cttgccagtt ttattttggc
     4261 gttgatgtac aagctaacca actgtcaaat aagagattat gatagattcg tcattggctc
     4321 ttttcaccag cagtcgcgtt cccctgccct ataaaaggag ggtatgcacc acgatggttc
     4381 attaccaata agattgaaat ctcatcactt tgttgaaatt ggcagcaaac aaacaaaaaa
     4441 atgcatttca ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga
     4501 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     4561 tccagtcaga ggagccaaat tcaaaaaagc ctgctttcta gcaggctttt tgctttctaa
     4621 ttaccagcgc tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga
     4681 tcgacgttcc aacgcagcat ttgcgcgatt cacatcaact tcttgcccgt tgataaacgc
     4741 ccgcaaagat ggggttaccg gcaatggcac ttttcggtca gactcatatt ctgcacgatt
     4801 gcgcgacagc ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt
     4861 cactggctga tcaataattt gtacacgagt gcctacagga acattatcaa acagatattt
     4921 gatatcgtca ttacgcagac gaatacagcc ctgacttaca cggagcccaa taccaaaatt
     4981 ggcattggta ccgtggatgg catacaacct gccaatataa atcgcgtaca gccccatggg
     5041 gttatcgggc cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg
     5101 ccgggtgttc ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac
     5161 ccagttacgc ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccactgt
     5221 attactgtct ggcggatagt aataaagacg catttcagcg acgttaacca caatcccttt
     5281 acgaacagtg gcgggcaaaa tcagttgctg cggaatggtg agttgtgagc caggcttcgg
     5341 caaaaaaaca tcagcgcccg gattcgcttc cagcatatta cttaaccctt gcccgtattg
     5401 tgcggcaaaa gtctccagcg gctgagtatt gtgatcagga acagttacag taagcgactg
     5461 ccccaccaaa cggctaccct ctggaggtaa cggataagtt accgccaggc tattttggct
     5521 ggcaaaaagc agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta
     5581 agtcgcgaat actatccgat aagtatagct tttatcagac ttttcgtttt tagctgttca
     5641 aatcagaagt cgtattcccc ggtagaacaa tattactggc agcaagttcg cccatgttgt
     5701 tgtatatcgc acaagcagct tcgatgatgg acatcgccag agctgcacca ctcccctcac
     5761 ctaaacgcat atccatattg agataaggct ccagccccaa atgcgagagc gctatacgcg
     5821 cgcctttttc tgccgacaag tgagaaggaa tgagatacgg tttgattgca ggagacatct
     5881 ggcaggctgc gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc
     5941 aggaagcagc acctaacatc actccagcta ttccgaccaa atcaaatcca cccacttttg
     6001 ccaggacatc aacaccatcc tgaggatttg gttgattcaa cgtaatcgcc cgacgcacaa
     6061 catcaatttt attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt
     6121 caggatcccg gccagtgatt gtgctgacta ttgccgctgc cggtgtcgtg tttgccatcc
     6181 ccagttcacc tacaccaaac agcgtgacac cattttttgc cagctcccgc gtataacata
     6241 tgacgtccaa aagcaacttt tcagcctgac ggcgactcat tgccggagct gaagcaatat
     6301 tgccgctacc tcgtgcgaca cgcatgttga taagcccggg gataggctca gcagtatcaa
     6361 taccaacatc aactacgtgg acgttagcgc ccgcttgtgc tgccagcaca cacacgccgg
     6421 ttgttccacg ggtcatattt tcagcctgta tggctgtcac ttcttttgga gaaatagcga
     6481 ccccttcctc ccagacgccg tgatcggcac acataaccag taccgctttt ttgcccacat
     6541 gcggtatgcc attcaacccc ggcattcctg ccagttgtat ggcaagcgcc tccagccttc
     6601 ccaggctacc aacaggtttg agtaacccgt caatatgccg ttgtgcacgc gacatagcgg
     6661 caggatcgat agcagggatc gtattcagta aatcggcaag tgtttgcatg tcacgtctct
     6721 ttatagcagt gccagcagga agaccagttc accaagctct atggctgctc ccagcgtatc
     6781 gcccgtttgc ccgcccagcg tacgtttgag aagctgaccg aggatgaaaa tcgccaccat
     6841 cgtcaccacc atagcagcca caccatgcat acccggcaat aatacggcag caaaaatagc
     6901 ggctaagccg agtgtgacgc aggtttgtcg cccatcaatt ttgccaatga acacattgcc
     6961 aagcccttct tcccgcgcat aacgatgacg atacatcaat aatgcggcag tcccgcggct
     7021 gaccgcacat gctgccgcca gcgatgcaag gatcggttcg ccacgcagag ccaactcgct
     7081 taacaccaga atctttgcca gtaccacaaa aatcaatgcc agaccgccgt gggtgcctaa
     7141 acggctatca cgcatgattt ccaacatgcg atcgcggcta cgtgcagaaa atacgccatc
     7201 acaggtatca gcgaggccgt ccagatggaa tcccccggtc atcagcgcca gcacaagtac
     7261 gctaaacagt gccgccagtg gtgcgccaca ccatgcctgc agcaccatga agaccagccc
     7321 gctaatcgcg ccaagcaata atccaatcaa aggaaaagta ataataccgc gagaataatg
     7381 ctcgaaatcc agtccctggg accagcgacg cggtacaggc agacgcgtaa taaatgagag
     7441 catcgcccaa aataatttgc tcatttgatt tttactccaa tacccgaaac caccagccat
     7501 acttcatttg ccgcagcggc caactgctga tttacccgcc cggcaatatc acgaaaatgt
     7561 cgtgccagac gactctccgg cacaatcccc attcccactt cgttagtcac taatacaacc
     7621 tttgcggggc aacgttggca ggcagcaatc aacgactgaa tctcagcatt aatcgcctgt
     7681 tccatcgcct gataatccca ttcatcaggg tctttatcgc cgccataatc aaacaacaga
     7741 ttagtcacca ttgtggtaac gcattccagc aacacagcct cattcgggtt aatgtctgca
     7801 tgaattaatt catcaagatg ttgccagcgc tccaccgtgc gccagtgctc cgggcggctt
     7861 tgccgatgat gttctatccg tgcagccatc tcatcatcaa ggatttgcga ggtagcgata
     7921 tacagaacct gtgaagagtc cccaataagc acctctgcgt ggcgactctt cccgctccgt
     7981 gcgcctcccg tcaccaaaat catcataccg actcctgatt caaatgtatg atggtattca
     8041 ttgaacagag caatggatcc cccaccgttg ctgaccgcaa aacatttacg cgattaatac
     8101 tgcgcgtaat ataattaaaa atccagcatt ctcaatccat tacgaaggta atgctgccaa
     8161 cttactgatt tagtgtatga tggtgatttt aaggtgcttg cgtggcttcc atttccatca
     8221 gatgtccttc ctgctccgct actgaaggcg tggtgcgtaa cggcaaaagc actgccggac
     8281 atcagcgcta tctctgctct cattgccgta aaacatggca actacagttc acttacaccg
     8341 cctctcagcc cggtacgcac cagaaaatca ttgatatggc catgaatggc gtcggatgtc
     8401 gcgccagtgc acgcattatg ggcgttggcc tcaacacggt tttacgtcac ttaaaaaact
     8461 caggccgcag tcggtaacct cgcgcataca accggg
//
LOCUS       DAJOSY010000151         8462 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJOSY010000151 DAJOSY010000000
VERSION     DAJOSY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.151"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(211..287)
                     /locus_tag="OHN27_003965"
     tRNA            complement(211..287)
                     /locus_tag="OHN27_003965"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Asp,seq:gtc)
     gene            complement(420..1151)
                     /gene="dnaQ"
                     /locus_tag="OHN27_003966"
     CDS             complement(420..1151)
                     /gene="dnaQ"
                     /locus_tag="OHN27_003966"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCQ2288315.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEEIAAHEARLDLVQKKGGSCLWRA"
     gene            1216..1683
                     /gene="rnhA"
                     /locus_tag="OHN27_003967"
     CDS             1216..1683
                     /gene="rnhA"
                     /locus_tag="OHN27_003967"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCQ2288316.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1680..2402)
                     /locus_tag="OHN27_003968"
     CDS             complement(1680..2402)
                     /locus_tag="OHN27_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCQ2288317.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGKLYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISFMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2436..3191
                     /gene="gloB"
                     /locus_tag="OHN27_003969"
     CDS             2436..3191
                     /gene="gloB"
                     /locus_tag="OHN27_003969"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCQ2288318.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3263..4621
                     /gene="mltD"
                     /locus_tag="OHN27_003970"
     CDS             3263..4621
                     /gene="mltD"
                     /locus_tag="OHN27_003970"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCQ2288319.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4669..5265)
                     /locus_tag="OHN27_003971"
                     /pseudo
     CDS             complement(4669..5265)
                     /locus_tag="OHN27_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(5296..6096)
                     /locus_tag="OHN27_003972"
     CDS             complement(5296..6096)
                     /locus_tag="OHN27_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCQ2288320.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6337..7251
                     /locus_tag="OHN27_003973"
     CDS             6337..7251
                     /locus_tag="OHN27_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2288321.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7248..8051)
                     /gene="dkgB"
                     /locus_tag="OHN27_003974"
     CDS             complement(7248..8051)
                     /gene="dkgB"
                     /locus_tag="OHN27_003974"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCQ2288322.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8214..8290)
                     /locus_tag="OHN27_003975"
     tRNA            complement(8214..8290)
                     /locus_tag="OHN27_003975"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8254..8256),aa:Asp,seq:gtc)
     gene            complement(8345..8460)
                     /gene="rrf"
                     /locus_tag="OHN27_003976"
     rRNA            complement(8345..8460)
                     /gene="rrf"
                     /locus_tag="OHN27_003976"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2265 a         2036 c         2053 g         2108 t
ORIGIN      
        1 ttaatcaata taaatttatt ctctagaaat atatttaagc tgaatggttc gatatcatta
       61 ttgaatttta atgaatgaga caacaagcga gaatcacgca cctacgccag agtaaaatca
      121 ttgaacagag aaatggtgga attcaaatac aaaaaaaccg caaaattaaa aatcttgcgg
      181 ctctctgaac tcattttcat gagtgaatag tggcggaacg gacgggactc gaacccgcga
      241 ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccgcg ttgtgttccg
      301 ttgggaacga ggcgaatatt acggattgcc tcgaccttcg tcaacggttt ttctcatctt
      361 ttgaatcgtt tgctgcaaaa atcgcccaag tcgctatttt tagcgccttt cacaggtatt
      421 tatgctcgcc agaggcaact tccgcctttc ttctgcacca gatcgagacg ggcttcatga
      481 gctgcaatct cttcatctgt cgcaaaaaca acgcgtaact tacttgcctg acgtacaatg
      541 cgctgaattg ttgcttcacc ttgttgctgt tgtgtctctc cttccatcgc aaaagccatc
      601 gacgtttgac caccggtcat cgccagataa acttccgcaa ggatctgggc atcgagtaat
      661 gccccgtgca gcgttcgttt actgttatct atttcgtagc gagcacataa cgcatcgagg
      721 ctgttgcgct taccgggaaa cattttcctc gccaccgcaa ggctatcggt gaccttacag
      781 aaagtattgg tcttcggaat atcgcgctta agcaacgaaa actcgtagtc cataaagccg
      841 atatcgaacg ctgcgttatg gatcaccaac tccgcgccgc gaatatagtc catgaactca
      901 tcggctactt cggcaaacgt gggcttatcg agcaaaaatt catcggcaat accatgtacg
      961 ccaaaggctt ccggatccac cagccgatcg ggtttgagat aaacatggaa gttattgccc
     1021 gtcaggcgac ggttcaccac ttcaacggca ccaatctcaa tgatcttgtg gccttcatag
     1081 tgcgcaccaa tctggttcat accggtggtt tcggtatcga gaacgatctg gcgtgtaatt
     1141 gcagtgctca tagcggtcat ttatgtcaga cttgtcgttt tacagttcga ttcaattaca
     1201 ggaagtctac cagagatgct taaacaggta gaaattttca ccgatggttc gtgtctgggc
     1261 aatccaggac ctgggggtta cggcgctatt ttacgctatc gcggacgcga gaaaaccttt
     1321 agcgctggct acacccgcac caccaacaac cgtatggagt tgatggccgc tattgtcgcg
     1381 ctggaggcgt taaaagaaca ttgcgaagtc attttgagta ccgacagcca gtatgtccgc
     1441 cagggtatca cccagtggat ccataactgg aaaaaacgtg gctggaaaac cgcagacaaa
     1501 aaaccagtaa aaaatgtcga tctctggcaa cgtcttgatg ctgcattggg gcagcatcaa
     1561 atcaaatggg aatgggttaa aggccatgcc ggacacccgg aaaacgaacg ctgtgatgaa
     1621 ctggctcgtg ccgcggcgat gaatcccaca ctggaagata caggctacca agttgaagtt
     1681 taagcctgtg gtttacgaca ttgccgggtg gctccaaccg cctggcgaat tcgtggcttg
     1741 tttttactct gtttcatcgg atttagcgtt aaaggaatag tccgtttccg ggcaacaata
     1801 agttgtaagc aaccaagcgc aggaatatgc gcattcaata gttttcctcc gtgtttgttc
     1861 cacgggagaa cgtggaaacg gctggcgtgt agcacttcaa aattcaacaa agagagccag
     1921 tccagctgcc gcatcagagt aaacatccgg ctgttatagg gcgaggtttt gcgcaatacc
     1981 ggcacaagtt tgcgtaatcc cataaaactg atgggattga agccactaat gaccagccag
     2041 ccatcatcaa tcaatacccg atcggcttca cgcaataaac gatgcggatc ggtgcaccac
     2101 ggcaatgtat gtgccagtag acaaacatca acggatttat cggcaaaagg aagatgaagt
     2161 gggtccgcct gtacctggac gggcattcct tgcgcagaaa cattcacttg atgagaaacc
     2221 gcgcacgctt cgcaattgat ttctgcgctt aaattgccaa tcttaagcag atgaaaacca
     2281 tacattttag tgaaccacgg gttgagctgg cgctccagcg ccttgcgata aagctttccc
     2341 cagggcaaat cgccccagca atcaggagcc acgacagttt gagggactct tgccggtttc
     2401 atcacaacct tccgtttcac actgagaggt aatctatgaa tcttaacagt attcccgcct
     2461 ttgatgacaa ttacatctgg gttttgaatg atgaagcagg tcgctgcctg attgtcgatc
     2521 ccggagacgc agagccagta ttaaacgcca ttgccgccaa taactggcaa ccggaggcca
     2581 tatttctcac ccaccatcac cacgatcacg ttggcggcgt aaaagaactg gtggaaaagt
     2641 ttccacaaat tgtggtgtat ggtccacaag agacacaaga taagggaaca acacaggtag
     2701 tcaaagatgg cgaaactgcc ttcgttttgg ggcatgaatt tagtgtaatt gctacgccgg
     2761 gtcacacttt aggacatatc tgttacttca gtaaacctta tctattttgc ggcgacacac
     2821 tgttttctgg tgggtgtggt cggttgtttg aagggacagc atcacaaatg tatcaatcac
     2881 ttaaaaagtt aagtgcgtta cctgacgata cattggtatg ttgtgctcat gaatatacct
     2941 tatcaaatat gaagtttgct ttgagtattc ttccgcacga tttgtccata aatgattatt
     3001 atcgtaaagt taaggagtta cgggcaaaaa atcaaataac actacccgta attctgaaaa
     3061 atgagcggca aattaatgtt tttttaagaa cggaagatat tgatttaatt aatgtaatta
     3121 atgaagaaac attattgcaa caacctgaag agcgttttgc atggttaagg tcaaagaaag
     3181 ataggttctg ataaaacttt cttgtcatcg gctccgttcg ccgttatgat cggtcgtctt
     3241 ttaagcaact attgacacac acatgaaggc aaaagcgata ttactcgcct ctgtcctgct
     3301 cgtgggttgc cagagtaccg gcaacgttca acagcacgca cagagccttt ctgcagctgg
     3361 tcaaggggaa gcagcaaagt ttacaagtca ggcacgatgg atggacgatg ggacgtctat
     3421 cgcgccagat ggtgacttgt gggctttcat tggcgacgag ctaaagatgg gaattccgga
     3481 aaatgaccgg attcgcgaac agaaacagaa atatttacgc aataagagct atctccacga
     3541 tgtaacttta cgggcagagc cgtatatgta ctggatagca gggcaagtta aaaaacgtaa
     3601 catgcctatg gaactggtac tactacccat agtggagagc gcttttgatc ctcacgcaac
     3661 gtctggcgcc aatgccgcag gcatctggca gatcattccg agcacggggc gcaattatgg
     3721 tttgaaacag acccgcaatt atgacgcgcg tcgcgatgtt gttgcttcaa caactgccgc
     3781 gctgaatatg atgcagcgtc tgaacaaaat gtttgacggc gactggcttc tgaccgtagc
     3841 ggcttataac agcggcgaag gtcgggtcat gaaggcaatt aaaacgaaca aagcgcgtgg
     3901 gaaatccacg gacttctggt cgttaccgtt gccgcaggaa acgaagcagt acgtgcctaa
     3961 aatgctggca ttgagtgata ttctcaaaaa cagcaagcgt tatggcgtac gtctgccaac
     4021 gaccgatgaa agccgtgctc tggcgcgtgt gcacctgagc agcccggttg aaatggcgaa
     4081 ggtagcagat atggcgggga tttccgtcag caagctgaag acattcaacg ctggcgtaaa
     4141 aggctccacg ctgggcgcaa gtggcccgca gtacgtgatg gtgccaaaga agcatgcaga
     4201 tcaactgcgt gaatctctgg cttcaggcga aattgctgct gtacagtcga cgctggttgc
     4261 cgacaatacg ccgcttaaca gccgtgttta caccgtacgc tctggcgaca cgctttcaag
     4321 tatcgcttca cgtctcggcg taagcaccaa agatttgcag cagtggaaca aactgcgcgg
     4381 atctaagctg aagccaggcc aaagtttgac gattggtgca ggcagtagcg cacagcgact
     4441 ggcaaacaac agcgatagca ttacgtatcg tgtgcgcaaa ggcgattcgc tttcaagcat
     4501 tgctaaacgc cacggcgtga acatcaaaga tgtgatgcgc tggaacagcg acactgcgaa
     4561 tctgcaacca ggcgataagc tgacgttgtt tgtgaaaaac aacaacatgc cagattcctg
     4621 acaaaccaga taataaaaag gcaccgattc ccccggtgcc ttttttattt atgccgcttt
     4681 atgtgcatct accatgatga tatcactggt gaaaaagcca tcattctgca aggcaaaata
     4741 cgttctcacc tctgtcgatg cgctctgttg gtaaatgcga atagcgtcta ctaacgcttc
     4801 tggcgtacgc attctcgcga cccatgaaga aaattccagc ggtaacttat ctgtaattaa
     4861 attatcaact atcagattgg cttcattgat taacgtcaac cactcaccgc tggcgtagtt
     4921 tcgtacgtga gaggtatcgc gtaatgcttc taccgtctgt aaccagatgt cgcgcactgg
     4981 gtgacccgga gacattacgt ccatcacaat cagcctaccg ccaggtttca atatcctatt
     5041 cacttctcgc agtgctgcac caacatcatg ccagtgatgg gcagaataac ggctgataac
     5101 aatatcaaat gcgttatcgg caaatggcag actttcggca tatccctggc gggtggtgat
     5161 atttttcagt tgccgggctt cggcagcttg tgccacgaca tccagcatgt gggcagataa
     5221 gtcatacgcc accaccgcgc tcacgttttg cgcagcgcca aacgttggtc tgccctgtgg
     5281 cagacctgac ataccttatt tatcaggctt gccgggactg aattcaacga gtagcggatt
     5341 gtgatcggaa gcgcgcgtaa ccagtacaga agcttcactg acgttcagac cacggtagaa
     5401 aacaaaatcg agcgggcgac caaacgcccg acggcgctga tcatcggtaa aacgcacctg
     5461 gcgcagcgac atttcccgcg caaagcgata taacgcgttc atccttctac ggctccaggc
     5521 attgaaatct cccgccataa tgaccgggcc gctgtggtga gctatctgat cgccaatagg
     5581 aagtaactgc ttactataga catccacgcc cagactgaag ttgacggcgt gtatattaac
     5641 caccatcaac aggcgggtgt caggtaatgg atagaccgtc accagtgccg acttcgccag
     5701 acgcaaaatg ggttctcgtt cacgtaacgg gcagcaatac actggatgtg ccgccgaaag
     5761 ggtcattacg ccagaaggat gttgtggcag cacgaaagcg ggtacctgat cggcggcaag
     5821 atagttagcg gtcgcaaact gtactaactc tggcgttgtc tgcgcttcct gcaataacac
     5881 cagatgtgca tctttgccgt agttctttaa taccgacaac cattcagcgc gttgctgttt
     5941 gtatatgttc cacaccagga tccgaatacg ctcttcggta cttaacggtt ccccaggtgg
     6001 taatgcctgg ccgatgctcg caaaagaccc cggcggtaag atcctttccg caggttgtcc
     6061 ggcaacatag cgcatggcat aggtgttttt tcgcactttt gactttgaaa cctctattac
     6121 gtcaaccctt ccgcactcgg gagggttcta ctgttatagg gattttagct cactctttca
     6181 acgagcaatt gtatattgtt atgtaagcaa gtgcttacac cggtcagaat tttgctcatt
     6241 atggagaaca ttaaatcgca tgacatatca cgcatcctga tggtttaatg acaaataaat
     6301 tcaaaatatc tcttctattt tgcaacagga gcaaaaatga aagccacgtc ggaagaactc
     6361 gccatttttg tttcggtcgt cgaaagcggc agctttagcc gggcagcgga acaattaggg
     6421 caagcaaact cagcggtaag ccgggcggtg aaaaagctgg agatgaaact tggcgttagc
     6481 ctgcttaatc ggaccacgcg acaacttagc ctgacggaag aaggcgagcg ttattttcgt
     6541 cgcgtacagt caattttgca ggagatggca gcggcagaat cagaaattat ggagacgcgt
     6601 aatacaccgc gtggactgtt acggatcgat gccgcaactc cagtggtgct gcactttctg
     6661 atgccgttaa ttaagccttt ccgtgaacgc tatccggaag tcactttgtc gctagtctcc
     6721 tccgaaacga ttattaattt gatcgaaaga aaagtggatg tcgcgatacg cgctggtacg
     6781 ttaacggatt ccagcttacg tgccaggccg ttatttaaca gttatcgaaa aattatcgcc
     6841 tcccccgatt atatttcccg ctacgggaag ccagaaacga tcgacgattt aaagcaacat
     6901 atttgcctgg gattcactga acccgcttcc ctcaatacct ggccgatagc ccgtagcgat
     6961 ggacaattac atgaggtgaa gtacggtttg tcatccaata gtggggaaac actgaaacag
     7021 ctttgcctga gtggcaacgg gattgcgtgt ttgtccgact acatgatcga caaagaaatc
     7081 gctcgcggag aattggtgga gttaatggca gataaagtgt tgccagtgga aatgccattc
     7141 agtgccgtct attacagcga ccgtgcggta agtacgcgca tccgggcttt tatcgatttc
     7201 cttagcgagc atgtaaaaac agctcccgga ggagctgtca gagaggctta atcccattca
     7261 ggagccagac cttccgggct aaccaggcgg tcgttgcaat ccagtgcggc gatcgctttt
     7321 ttatcttcgg catcaagctg taaattttgt gccttaagat tactttccag gtttttacgt
     7381 ttagtagaag aaggaattac tgagtaacct tcccccatag cccacgccag aatcacttgt
     7441 gccggagtcg cattgtgttt agctgcgata cgagcaataa cctcatcttt cagggcctta
     7501 ccatacgcca gcgtcatata ggaagtaata tggatgccgt gctgtttagc ccaggcaacc
     7561 actttacggt tttgcagata aggagagagt tcaatctggt tagtagcgat gttttcagca
     7621 ccaacagcag caatcgcttt ttccatcaac gggatcgtga agttggaaat accgatctca
     7681 cgcgtcagcc cttgtttttt ggcttccagc agcgcctgca taaactcttc aacagagact
     7741 tcatcgtttg gtgacggcca gtggattagc gtcagatcaa cataatcggt acgcaatttt
     7801 tgcaggctct ctttcagact tgggatcaat ttgtctttgc tgagattttc aatccagatt
     7861 ttagtggtga tgtagagttc atgacgtggc acgccacttt ctgcaatcgc ctgacctact
     7921 gcggcttcgt tatcatagat ttgtgcggta tcaattgcgc gataaccaag ttcaagcgcc
     7981 gttatcacag atgaaataac aacgtcgtct ttcagacgga aagtacctaa accaaatgca
     8041 gggatagcca taatatgcct cttttaatgc tattttcgtt aactgaggtg attatgggct
     8101 tcagactgtt gaggaaaaag agcgtaaaat gcagaggatt tttgcgattc tggcaataat
     8161 agatatacag acgaaaaaaa acctcgagcg ttaactcgag gcttcttaat aagtggcgga
     8221 acggacggga ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa
     8281 ctaccgctcc accgaattct tttacaacca ccggttttat gaccggctta ctgcttaatt
     8341 tgatgcctgg cagttcccta ctctcgcatg gggagacccc acactaccat cggcgctacg
     8401 gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac ggccgccagg
     8461 ca
//
LOCUS       DAJOSY010000152         8442 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJOSY010000152 DAJOSY010000000
VERSION     DAJOSY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.152"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            58..948
                     /gene="alsR"
                     /locus_tag="OHN27_003977"
                     /gene_synonym="rpiR"
     CDS             58..948
                     /gene="alsR"
                     /locus_tag="OHN27_003977"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HCQ2288323.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            1007..1942
                     /gene="alsB"
                     /locus_tag="OHN27_003978"
     CDS             1007..1942
                     /gene="alsB"
                     /locus_tag="OHN27_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HCQ2288324.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            2066..3598
                     /gene="alsA"
                     /locus_tag="OHN27_003979"
     CDS             2066..3598
                     /gene="alsA"
                     /locus_tag="OHN27_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HCQ2288325.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNISYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGIVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHETVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            3577..4557
                     /gene="alsC"
                     /locus_tag="OHN27_003980"
     CDS             3577..4557
                     /gene="alsC"
                     /locus_tag="OHN27_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HCQ2288326.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            4568..5263
                     /gene="alsE"
                     /locus_tag="OHN27_003981"
     CDS             4568..5263
                     /gene="alsE"
                     /locus_tag="OHN27_003981"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HCQ2288327.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            5247..6176
                     /gene="alsK"
                     /locus_tag="OHN27_003982"
     CDS             5247..6176
                     /gene="alsK"
                     /locus_tag="OHN27_003982"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HCQ2288328.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLRDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLVAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQFCAKVP"
     gene            6449..8434
                     /gene="yjcS"
                     /locus_tag="OHN27_003983"
     CDS             6449..8434
                     /gene="yjcS"
                     /locus_tag="OHN27_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCQ2288329.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMGNGDNLNLTLNDSVLNYRKTLQPQANASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         2186 a         2058 c         2160 g         2038 t
ORIGIN      
        1 ttctgttctt gctgtggtga tatagtggcg tcttcaattc aaggacaaga gaacgtgatg
       61 agccagtcag agtttgattc agcgcttccg aacggtatag ggttagcgcc ttacctgcga
      121 atgaagcagg aaggaatgac agaaaatgaa agccgcatcg tggagtggtt actcaaaccc
      181 ggtaacctga gttgtgcacc cgcaattaaa gatgtcgcag aagctctggc ggtatctgaa
      241 gcgatgatag ttaaggtatc aaagctgctg gggtttagcg gctttcgtaa cttacgcagt
      301 gcgctggaag attatttttc tcagtcagaa caggtattgc cttccgagtt ggcttttgat
      361 gaagcgccgc aggatgtggt gaataaggta tttaacatca ctttacgcac cattatggaa
      421 ggtcagtcga tcgtcaacgt tgatgagatc caccgtgccg cccgcttttt ctatcaggcc
      481 agacagcggg atttgtacgg tgccggagga tcaaatgcta tctgtgctga tgtacagcac
      541 aagttcttgc gcattggcgt acgctgtcag gcctatcctg atgctcacat catgatgatg
      601 tccgcttcgt tgttacagga aggagatgtt gtgctggtag tgacccattc cgggcgaacc
      661 agtgatgtaa aagcggccgt agaactggca aaaaagaacg gggcaaagat tatttgtata
      721 acccatagct accattcacc gatagcgaaa ctggccgatt atattatttg ctcaccagcc
      781 ccggaaacgc cgttattagg tcgtaatgcc tcggcaagaa tattacaact aactttgctg
      841 gacgcttttt ttgtctctgt cgcccagctc aacattgaac aagctaatat taatatgcaa
      901 aaaaccggcg caattgttga tttcttctca ccaggcgcgc tgaaataaga aaaaggtaat
      961 tccgccatcg cggagttacc tgtcccctta ccgtgagaac attattatga ataaatatct
     1021 gaaatatttc agcggcacac tcgtgggctt aatgttgtca accagcgctt ttgctgccgc
     1081 cgaatatgct gtcgtattga aaaccctctc caacccattt tgggtagata tgaaaaaagg
     1141 cattgaagat gaagcaaaaa cactgggcgt cagcgttgat atttttgcct ctccttcaga
     1201 aggcgatttt caatctcaat tgcagttatt tgaagatctc agtaataaaa attacaaagg
     1261 tatcgccttc gctccattat cctcagtgaa tctggtcatg cctgtcgccc gcgcatggaa
     1321 aaaaggcatt tatctggtta atctcgatga aaaaatcgac atggataatc tgaaaaaagc
     1381 tggcggcaat gtggaagctt ttgtcaccac cgataacgtt gctgtcgggg cgaaaggcgc
     1441 gtcgttcatt attgacaaat tgggcgctga aggtggtgaa gtcgcaatca ttgagggtaa
     1501 agccggtaac gcctccggtg aagcgcgtcg taatggtgcc accgaagcct tcaaaaaagc
     1561 aagccagatc aagcttgtcg ccagccagcc tgccgactgg gaccgcatta aagcactgga
     1621 tgtcgccact aacgtgttgc aacgtaatcc gaatattaaa gcgatctatt gcgcgaatga
     1681 cacgatggca atgggtgttg ctcaggcagt cgcaaacgcc ggaaaaacgg gaaaagtgct
     1741 ggtcgtcggt acagatggca ttccggaagc ccgcaaaatg gtggaagccg gacaaatgac
     1801 cgcgacggtt gcccagaacc cggcggatat cggcgcaacg ggtctgaagc tgatggttga
     1861 cgctgagaaa tccggcaagg ttatcccgct ggataaagca ccggaattta aactggtcga
     1921 ttcaatcctg gtcactcaat aaaactgaat gatgccggat gatgctaatg aagtgtctta
     1981 tccggccaac aaaataacct gaacgtagac ctgataaatg tagccatcag gcaataatac
     2041 gatatttcgt gcagagaggt tatttatggc cacgccatat atatcgatgg cggggatcgg
     2101 caagtccttt ggtccggttc acgcattaaa gtcggttaat ttaacggttt atcctggtga
     2161 aatacatgca ttactaggag aaaatggcgc gggtaaatcc acgctaatga aagttttatc
     2221 cggaatacat gagccgacca aaggcaccat taccattaat aacattagct ataacaagct
     2281 ggatcataaa ttagcggcac aactcggtat cgggattatt tatcaggaac tcagcgttat
     2341 tgatgaatta accgtactgg aaaatttata tattggtcgt catctgacga aaaaaatctg
     2401 tggcgtcaat attatcgact ggcgagaaat gcgtgtccgc gccgccatga tgttattacg
     2461 cgtgggcttg aaagttgatc tagatgagaa agtggcgaat ttatctatca gccacaagca
     2521 gatgctagaa attgccaaaa cgctgatgct cgatgccaaa gtcatcatca tggatgaacc
     2581 cacctcctca ctcaccaata aagaggtgga ctatctgttt ctgatcatga atcagttgcg
     2641 taaagagggt acggccatcg tctatatctc gcataagttg gcggaaattc gccgtatttg
     2701 cgaccgctat acggtgatga aagacggcag cagcgtttgc agcggcatag taagcgatgt
     2761 gtcaaatgac gatatcgtcc gtctgatggt aggccgcgaa ctgcaaaacc gttttaacgc
     2821 gatgaaggag aatgtcagca accttgcgca cgaaacggtt tttgaggtgc ggaacgtcac
     2881 cagtcgtgac agaaaaaagg tccgggatat ctcatttagc gtctgccggg gagaaatatt
     2941 aggctttgcc ggactggtcg gttccggacg tactgaactg atgaattgtc tgtttggcgt
     3001 ggataaacgc gctggcggag aaatccgtct taatggcaaa gatatctctc cacgttcacc
     3061 cctggatgcc gtgaaaaaag ggatggctta catcactgaa agccgccggg ataacggttt
     3121 tttccccaac ttttccatcg ctcagaacat ggcgatcagc cgcagtctga aagacggcgg
     3181 ctataaaggc gcgatgggct tgtttcatga agttgacgag caacgtaccg ctgaaaatca
     3241 acgcgaactg ctggcgctga aatgtcattc ggtaaaccag aatatcaccg aactctccgg
     3301 gggaaatcag cagaaagtcc tgatctccaa atggctgtgc tgttgcccgg aagtgattat
     3361 tttcgatgaa cctacccgcg gcatcgacgt tggcgcgaaa gccgaaattt acaaagtgat
     3421 gcgccaactg gcggacgacg gaaaagtcat cctgatggtg tcatctgaac tacctgaaat
     3481 tatcaccgtc tgcgaccgca tcgccgtgtt ctgcgaagga cgactgacgc aaatcctgac
     3541 gaatcgcgat gacatgagcg aagaggagat tatggcatgg gctttaccac aagagtaaaa
     3601 agcgaagcga gcgagaagaa accgttcaac tttgcgctgt tctgggataa atacggcacc
     3661 ttttttatcc tggcgatcat cgtcgccatc tttggttcgc tgtcaccaga atattttctg
     3721 accaccaata atattaccca gatttttgtt caaagctccg tgacggtatt gatcggcatg
     3781 ggcgagtttt tcgctatcct ggtcgctggt atcgacctct cggttggcgc gattctggcg
     3841 ctttccggta tggtgaccgc caaactgatg ttggcaggtg ttgacccgtt tctcgcagcg
     3901 atgattggcg gtgtactggt tggcggcgca ctgggggcga tcaacggctg cctggtcaac
     3961 tggacggggc tacacccgtt catcatcacc cttggcacca acgcgatttt ccgtgggatc
     4021 acgctggtga tctccgatgc caactcggta tacggcttct catttgactt cgtgaacttc
     4081 tttgccgcca gcgtaattgg gatacctgtc cccgttatct tctcactaat tgtcgcgctc
     4141 atcctttggt ttctgacaac gcgtatgcgg ctcgggcgca acatctacgc actgggcggc
     4201 aacaaaaatt cggcgttcta ttccgggatt gacgtgaaat tccacatcct ggtggtgttt
     4261 atcatctccg gtgtttgtgc aggtctggca ggcgtcgtct caactgcacg actcggtgcc
     4321 gcagaaccgc ttgccggtat gggttttgaa acctatgcca ttgccagcgc catcattggc
     4381 ggcaccagtt tcttcggcgg caaggggcgc attttctctg tggtgattgg cgggttgatc
     4441 atcggcacca tcaacaacgg tctgaatatt ttgcaggtac aaacctatta ccaactggtg
     4501 gtgatgggcg gattaattat cgcggctgtc gcccttgacc gtcttatcag taagtaagga
     4561 attgaacatg aaaatctccc cctcgttaat gtgtatggat ctgctgaaat ttaaagaaca
     4621 gatcgaattt atcgacagcc atgccgatta cttccacatc gatatcatgg acggtcactt
     4681 tgtccccaat ctgacactct caccgttctt cgtaagtcag gttaaaaaac tggcaactaa
     4741 accgctcgac tgtcatctga tggtgacgcg gccgcaggat tacattgctc aactggcgcg
     4801 tgcgggagca gatttcatca ctctgcatcc ggaaaccatc aacggccagg cgttccgcct
     4861 gattgatgaa atccgccgtc atgacatgaa agtggggctg atccttaacc cggagacgcc
     4921 agttgaggcc atgaaatact atatccataa ggccgataaa attacggtca tgactgtcga
     4981 tcccggcttt gccggacaac cgttcattcc tgaaatgctg gataaacttg ccgaactgaa
     5041 ggcatggcgt gaacgagaag gtctggagta cgaaattgag gtggacggtt cctgcaacca
     5101 ggcaacttac gaaaaactga tggcggcagg ggcggatgtc tttatcgtcg gcacttccgg
     5161 cctgtttaat catgcggaaa atatcgacga agcatggaga attatgaccg cgcagattct
     5221 ggctgcaaaa agcgaggtac agcctcatgc aaaaacagca taacgtcgta gcgggcgtgg
     5281 atatgggggc aacgcatatc cgcttttgtc tgcggacagc agaaggtgaa acgctacact
     5341 gcgaaaaaaa gcggaccgca gaagtcattg ctcccggcct ggtgtcgggt atcggcgaaa
     5401 tgattgacga gcaactcagg cgctttaacg ctcgctgtca tggtctggtg atgggatttc
     5461 cggcgctggt cagtaaagat aaacgcacca ttatttctac gcctaacctg ccgttaacag
     5521 cggcggattt atatgatctc gccgataagc tcgaaaatac gctgaattgt ccggttgagt
     5581 tttcccgcga cgttaacctg caactctcct gggacgtagt agaaaaccgc cttacgcaac
     5641 aactggttct ggcggcctat ctcggtacgg ggatggggtt cgcagtgtgg atgaacggtg
     5701 cgccgtggac gggtgcacac ggtgtggcag gcgaactggg tcatatcccc ctgggagata
     5761 tgacccaaca ctgcgcgtgt ggcaatcctg ggtgcctgga aaccaattgc tctggaatgg
     5821 cgctaagacg ctggtacgaa caacagcccc gaaattaccc attgcgcgat cttttcgtcc
     5881 atgcggaaaa cgcccctttc gtccagagtc tgcttgaaaa cgcggcacgg gccattgcca
     5941 ccagcattaa tctgttcgat cccgatgcgg tgatcctggg cggtggcgtg atggatatgc
     6001 ccgccttccc acgcgagact ctcgttgcca tgacccaaaa gtacctgcgc cgtccactgc
     6061 cgcatcaggt cgtgcgcttt attgccgcct catcttctga ctttaatggc gctcagggtg
     6121 cagcaatatt ggcgcatcaa cgttttttgc cacagttctg tgctaaagtc ccatgagtaa
     6181 aacgttggca cagtagcgca atgtcccgct gtgccaacga caggtacaat atatataata
     6241 aacacataaa acgactcata ttattttctg caatgatagt tttactgtaa ttttccctct
     6301 tcagcacaaa tgcctaataa aatggcgcgt tccgctcaca cttccactta ttaaagtcgc
     6361 aatttcccaa ttatgataac gccagcttta aatgacgtat tttgcatgga ttacatattt
     6421 acgacataaa tgataaatgg agatttatat gaataactct cggttattcc gtttgagcag
     6481 gattgttatt gcgttaactg ccgccagcgg catgatggta aataccgcta acgcgaaaga
     6541 ggaagcgaaa gccgccactc aatataccca acaggttaat cagaattacg ccaaatcatt
     6601 accgtttagc gatcgtcagg attttgacga tgcccagcgt ggatttatcg ccccgctgct
     6661 ggatgaaggt attctgcgtg atgcgaacgg taaagtttac taccgcgcgg acgattacaa
     6721 atttgatatt aatgccgcag cgccggaaac cgtaaacccc agcctgtggc gtcagtcgca
     6781 aatcaacggt atttctggcc tgttcaaagt caccgataaa atgtatcagg tgcgcggcca
     6841 ggatatctct aacattacgt tcgttgaggg cgagaaaggc attattgtta tcgacccgct
     6901 ggtgacgccg cctgccgcaa aagccgcact tgacctttac ttccagcatc gtccgcaaaa
     6961 accgattgtt gccgttatct acactcacag ccacaccgac cactatggtg gcgtgaaagg
     7021 cattatctct gaagccgatg ttaaatccgg caaagttcag gtgattgccc ctgcaggctt
     7081 tatggacgaa gccatcagcg aaaacgtgct ggcgggtaac atcatgagcc gccgtgcgct
     7141 ctactcttac ggtctgttac tgccgcacaa cgcgcaaggc aacgtgggta atggccttgg
     7201 cgtgacgctg gcaacgggcg acccgagcat tattgcacct actaaaacta tcgtccgaac
     7261 tggcgagaag atgattatcg acggcctgga gtttgacttc ctgatgaccc caggtagcga
     7321 agcgccagcc gaaatgcact tctatattcc ggccctgaaa gccctgtgta ccgccgagaa
     7381 cgccacgcat accctgcaca acttctacac tctacgcggc gcgaaaaccc gcgacaccag
     7441 caagtggacc gagtatctga acgaaacgct ggatatgtgg ggtaacgacg cggaagtcct
     7501 gtttatgccg cacacctggc cggtctgggg caataagcat atcaatgatt atattggtaa
     7561 atatcgcgat actatcaagt acattcacga ccagaccctg cacctggcga accagggcta
     7621 caccatgaat gaaatcggcg acatgattaa gctgccgcct gcacttgcca ataactgggc
     7681 cagccgtggc tattacggtt ctgtcagcca caacgcccgc gcggtgtata acttctatct
     7741 tggctattac gacggtaacc cggctaacct gcatccgtat ggtcaggtgg agatgggtaa
     7801 acgttacgtg caggcgctgg gcggttctgc ccgtgtcatc aacctggcgc aagaagcgaa
     7861 caagcaaggt gattaccgct ggtcggcaga actgctgaaa caggtgattg ccgccaaccc
     7921 gggtgaccag gtcgcgaaga atctgcaagc gaataacttt gaacagctgg gctatcaggc
     7981 cgagtccgcc acctggcgcg gtttctacct gaccggcgcg aaagagctgc gcgaaggggt
     8041 gcataagttc agccacggca ccaccggttc cccggacacc attcgcggga tgtcggtcga
     8101 aatgctgttc gactttatgt ccgttcgcct cgatagcgcg aaagccgcgg gtaaaaatat
     8161 cagcctgaac ttcaatatgg gcaacggcga taacctcaac ctgacgctga acgatagtgt
     8221 gcttaactac cggaaaacgc tgcaaccgca agccaacgcc tctttctaca tcagccgtga
     8281 agatctgcac gccgtgctga ccggacaggc aaaaatggcg gatctggtaa aagcgaagaa
     8341 agccaaaatt attggcaatg gcgcgaaact ggaagaaatt attgcctgtc tggataattt
     8401 cgatttgtgg gtgaatatcg taaccccaaa ttaatgccac tc
//
LOCUS       DAJOSY010000153         8339 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJOSY010000153 DAJOSY010000000
VERSION     DAJOSY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.153"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..252
                     /locus_tag="OHN27_003984"
     CDS             <1..252
                     /locus_tag="OHN27_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288330.1"
                     /translation="FILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEM
                     LPVNLTQETLFSSKSHGKYALFPIFTASWRAHRIMNKGV"
     gene            475..972
                     /gene="ubiC"
                     /locus_tag="OHN27_003985"
     CDS             475..972
                     /gene="ubiC"
                     /locus_tag="OHN27_003985"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCQ2288331.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            985..1857
                     /gene="ubiA"
                     /locus_tag="OHN27_003986"
     CDS             985..1857
                     /gene="ubiA"
                     /locus_tag="OHN27_003986"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCQ2288332.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2012..4435)
                     /gene="plsB"
                     /locus_tag="OHN27_003987"
     CDS             complement(2012..4435)
                     /gene="plsB"
                     /locus_tag="OHN27_003987"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCQ2288333.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4606..4974
                     /gene="dgkA"
                     /locus_tag="OHN27_003988"
     CDS             4606..4974
                     /gene="dgkA"
                     /locus_tag="OHN27_003988"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCQ2288334.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5084..5692
                     /gene="lexA"
                     /locus_tag="OHN27_003989"
     CDS             5084..5692
                     /gene="lexA"
                     /locus_tag="OHN27_003989"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCQ2288335.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5765..7090
                     /gene="dinF"
                     /locus_tag="OHN27_003990"
     CDS             5765..7090
                     /gene="dinF"
                     /locus_tag="OHN27_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCQ2288336.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQELADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7206..7415
                     /locus_tag="OHN27_003991"
     CDS             7206..7415
                     /locus_tag="OHN27_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCQ2288337.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7457..7972)
                     /gene="zur"
                     /locus_tag="OHN27_003992"
     CDS             complement(7457..7972)
                     /gene="zur"
                     /locus_tag="OHN27_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCQ2288338.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1901 a         2030 c         2275 g         2133 t
ORIGIN      
        1 tttatcctgt tctcctcagt cgatgatggg ccagttatgg tggtaagctc ccagcgctta
       61 catgatatgt tgaatcctac aaaagatacc aattggaatt ccacgtatat ctacaaatcc
      121 agacatgaga tgttgcctgt taatcttact caggaaacac ttttcagctc caaatctcat
      181 ggtaaatatg cgcttttccc aatttttact gcgagttggc gagctcatcg tataatgaat
      241 aagggtgttt aagtaaagga aaacatcacc gttcctggca tcctggacgg tgatgcccct
      301 acggttgccc tcgccagcac gggcatcggt aaagcgtaag gttcaacatc gttttaccac
      361 ttcatgcgat tgttgcgttt ttgttgcgta ttagatcact taatttgctt tacatctccc
      421 gtaaacactt ttctgcgata caatgccttt acgttatgta acggagagtt cggcatgtca
      481 caccccgcgt taacgcaact gcgtgcgctg cgctatttta aagagatccc tgccctggat
      541 ccgcaactgc tcgactggct gttgctggag gattccatga caaaacgttt tgaacagcag
      601 ggaaaaacgg taagcgtgac gatgatccgc gaagggtttg tcgagcagaa tgaaatcccc
      661 gaagaactgc cgctgctgcc gaaagagtct cgttactggt tacgtgaaat tttgttatgt
      721 gccgatggtg aaccgtggct tgccggtcgt accgtcgttc ctgtgtcaac gttaagcggg
      781 ccggagctgg cgttacaaaa attgggtaaa acgccgttag gacgctatct gttcacatca
      841 tcgacattaa cccgggactt tattgagata ggccgtgatg ccgggctgtg ggggcgacgt
      901 tcccgcctgc gattaagcgg taaaccgctg ttgctaacag aactgttttt accggcgtca
      961 ccgttgtact aagaggaaaa aaatatggag tggagtctga cgcagaataa gctgctggcg
     1021 tttcatcgct taatgcgtac ggataagcca attggcgcgt tactgctgct ctggccaaca
     1081 ttatgggcgt tgtgggtggc gacaccgggc gttccccagc tctggatcct ggcggtgttt
     1141 gtcgcgggtg tctggctgat gcgcgctgcc ggatgtgtgg tgaatgatta tgctgaccgc
     1201 aagtttgatg gtcatgttaa gcgcacggcg aaccgaccac ttcccagcgg cgcggtaaca
     1261 gagaaagagg cgcgcgcgct gtttgtcgtg ctggtactga tttcgttttt actggtgctg
     1321 acgctgaata cgatgaccat tctgttgtcg attgccgcgc tagcgctggc gtgggtgtac
     1381 ccgtttatga agcggtatac ccatctaccg caagtggtgc tgggcgcggc gtttggctgg
     1441 tcgattccaa tggcttttgc cgctgtgagt gagtcggtgc cattgagttg ctggttaatg
     1501 ttcctcgcca atattctctg ggcggtggct tacgacacgc agtatgcgat ggttgaccgc
     1561 gatgatgatg tgaagattgg cattaaatcc acggcaatcc tgttcggcca atacgataaa
     1621 ttgattattg gtattttgca gattggcgta ctggcactga tggcgatcat cggtgagtta
     1681 aatggcttag gctggggata ttactggtca attctggtgg ctggcgcgct gtttgtttat
     1741 caacaaaaac tgattgccaa ccgcgagcgt gaagcctgct ttaaagcatt tatgaataat
     1801 aactatgttg gtctggtact atttttaggg ctggcaatga gttactggca tttctgatga
     1861 tgtaaaaaag ccggatgatc atccggcttt cttctgggtt gcctgatgcg cggcgcttct
     1921 caggcctaca caacacatcg caatttattg aatttgcaga ttatggaagg ccggataagg
     1981 cgttttcgcc gcatccggca attctctctg attacccttc gccctgcgtc gcactctcaa
     2041 tcgtcaaacg cacgtctgat gtaatcaact ccgccagcaa ctgataaacc ttcatcgttt
     2101 ctgccggttc ggcatcgccg ctatcgctga tatacccttc atcacgcagt gtcagcacca
     2161 gagaactgaa caccgccttg tcgaagaact ccggcgcgtt gatgccgtgc agcacggaga
     2221 gacgttgcgc gacggtgcgg ctctctttct ccagcgtacc gcggttgatc gacgggttgg
     2281 cactcaacaa ccagaaggtg atggcataac gttgcagcgt ttcgcgcgcg cctgcggcca
     2341 gcagctgtag cgtgcgagaa tgcgccgggt tgatatgcaa ctcatcatct tgcagggtaa
     2401 tcagcccctg acgttgcatc tcatttgcca gcgcatcaat aacgtccggc aactcgtcgc
     2461 gatcccagcg caggaacagc tccgctttca gcattgggta aagcacattg acgtgctcca
     2521 tcaatacgtc gcgggagatg tggcgatgct gggtgacgat tgccgccatc agcgaaggca
     2581 gcaccaacat atgcgcaatg ttgttgcgat agtaggtcat cagcaccgct tgctcgcgcg
     2641 gcagaatgat gatgtcgccg attgtgtctt tctcgacttc aaacttgttc atttgcagcg
     2701 cgtgatcgat aagctcgctg gcgctggctg aaggaacggt agagtccgtg gagtaaggta
     2761 cgttgcgcat cagatccagg tagcagttga gttgctcggt taactgctcg cgggtgagtg
     2821 agcgctgacg tgacgccaga agcgcagtac agcacaggtt catggcgttt gccgcgcctg
     2881 cgttgttaat gcgtaccatc agatcggcag caatattatt gaccgtcggc gttaaccatg
     2941 ccggacgcac cgcttcgatg ggatcgatag attcacgcca gtctggtacg tgctggttaa
     3001 ggtaggtcat caacggcatt ggttcaccga agttgacgta accctgaccg agattacgca
     3061 gcttgcttaa accgcgcagc atctgcggca ggctctcttt ctctttcgtc gcgccgcgca
     3121 gttctttggc gtaagtaccc acttccatga cgtgctcata accgatgtag atcggaatca
     3181 gcgtaatcgg acgcgtgccg ccacgcagca tcgcctgaat ggtcatcgac agcgtaccgg
     3241 ttttcggatc cagcaaacgc cccgtgcggg aacgaccgcc ttccacgaag tactcgacgg
     3301 aataaccacg gctgaacagt tcgccgagat actcacggaa aacggtggaa taaagtttat
     3361 tgcctttaaa agtacggcga ataaagaacg cccccagacg gcggaaaatc ggcccggccg
     3421 gccagaagtt caggttgatc ccagcggcga tatgcggcgg caccagcccc tggtgataca
     3481 gcacgtaaga aagcagcagg tagtccatgt ggctgcggtg gcaaggcaca tataccagct
     3541 catgaccgtc gtgggccagc tggcgaacgc gctcggcgtt atggacgttg atgccctggt
     3601 aaaggcggtt ccaggtgaag cccagaatac ggtcagtcag gcgaatcatc tcgtaagaga
     3661 aattcgccgc aatctcttcc atcagcgcaa tcgcgttctg ctgcgctttt tcatgggaga
     3721 tttttttgct gcgcgcttca tcttctaccg ctttggcaat ggcgcgggag gcgagcagct
     3781 tattaaacag atcctgacga gcaggcagac gtgggcccac agcagccaga cgttgacggg
     3841 caaagtgcat acgcgccacg cgcgccagtt tctgtgcgat agttttatcc gtgccgtgtt
     3901 catccgccat acggcgcagc gaaactgacg gcgagaaacg cacaaaactg tcgcgaccga
     3961 gccacagtac ggcgaaaaat ttctgtacgc cgttaagcat acgcagcggc gggtttactt
     4021 cgcctttttc acgccccggc gcgcgaccaa acatcaccga tactggcacc atctgcacat
     4081 ccagatttgg gttgctacgg tgcaaatcga gatagtcgtg gaacagctta atagactctt
     4141 ctttcggcgt gtaataggtg aatacacgcg gcccgccgtg aatgaacaca tagcgcggca
     4201 gtagcgtgcc gtcgatttcc agcggctcta acgggtcagg caagtcatga gccagacact
     4261 gggcgcgtaa cgtcagcaaa tctgctttcg agttgtacgg taaaacgtac ataattggac
     4321 gagaggtatc cagccccagt tccggggcag gatctgccgg aatagacttg ctttttacca
     4381 ggatgcttaa tggtaaattc agtaatttgt agtaaattcg tggccagccg gacataaacg
     4441 atgtaaagcc tctggttaat aatgcaaatg cgcggcaagg atagcagaaa gtcatgggaa
     4501 attctgtggt atccgctcat gtttcgcgcg gcgttacgca aacccgaatc atcggattta
     4561 acggtacact gatattgacg ctcataatgt aaaaaggttc tttcaatggc caataatacc
     4621 actggattca cccgaattat caaagctgct ggctattcct ggaaaggttt acgcgctgca
     4681 tggatcaacg aagcggcatt ccgtcaggaa ggcgtagcgg tattgttggc ggtggtcatc
     4741 gcctgctggc tggatgtgga cgcaattacc cgcgtgctgc ttatcagctc cgtgatgctg
     4801 gtgatgattg tggaaatcct caatagcgcc atcgaagcag tggttgaccg aattggctct
     4861 gaataccatg agctttccgg acgcgctaaa gatatgggat ccgccgcggt gctgattgcg
     4921 attatcgtcg ccgtgataac ctggtgcatc ctgttatggt cgcattttgg ataacccttc
     4981 cagatttcga taaatctctg gtttattgtg cagtttatgg ttccaaaatc gccttttgct
     5041 gtatatactc acagcataac tgtatataca cccagggggc ggaatgaaag cgttaacggc
     5101 caggcaacaa gaggtgtttg atctcatccg tgatcacatc agccagacag gtatgccgcc
     5161 gacgcgtgcg gaaatcgcgc agcgtttggg gttccgttcc ccaaacgcgg ctgaagaaca
     5221 tctgaaggcg ctggcacgca aaggcgttat tgaaattgtt tccggcgcat cacgcgggat
     5281 tcgtctgttg caggaagagg aagaagggtt gccgctggta ggtcgtgtgg ctgccggtga
     5341 accgcttctg gcgcaacagc atattgaagg tcattatcag gtcgatcctt ccttgttcaa
     5401 gccgaatgct gatttcctgc tgcgcgtcag cgggatgtcg atgaaagata tcggcattat
     5461 ggatggcgac ttgctggcag tgcataaaac tcaggatgta cgtaacggtc aggtcgttgt
     5521 cgcacgtatt gatgacgaag ttaccgttaa gcgcctgaaa aaacagggca ataaagtcga
     5581 actgttgcca gaaaatagcg agtttaaacc aattgtcgtt gaccttcgtc agcagagctt
     5641 caccattgaa gggctggcgg ttggggttat tcgcaacggc gactggctgt aacatatctc
     5701 tgagaccgcg atgccgccag gcgtcgcggt ttgtttttca tctctcttca tcaggcttgt
     5761 ctgcatggca ttcctcactt catctgataa agcactctgg catctcgcct tacccatgat
     5821 tttctccaat atcaccgttc cgttgctggg actggtcgat acggcggtaa ttggtcatct
     5881 tgatagcccg gtttatttgg gcggcgtggc ggttggtgca acggcgacca gctttctctt
     5941 tatgctgttg ctgtttttac gcatgagcac caccgggctg actgcgcagg cttatggtgc
     6001 caaaaatcct caggcattag cccgtgcgct ggtgcaaccg ttgctgttgg cgttgggggc
     6061 tggggcgtta attgcgctgc tgcgtacacc gattatcgat ctggcgctgc atattgttgg
     6121 cggtagtgaa gcggtcctgg aacaggcgcg gcgctttctt gaaatccgct ggttaagcgc
     6181 tccggcgtcg ctggcgaatc tggtattact cggttggtta ctcggcgtgc aatatgcccg
     6241 tgcgccagta attttgttag tggtcggcaa tatcctcaac attgtgctgg atgtctggct
     6301 ggtgatgggg ctgcatatga acgtgcaggg cgcggcgctg gcgacggtta ttgcggaata
     6361 tgcaacattg ctgattggtc tgctaatggt gcgtaaaatc ctcaaactac gcggaatttc
     6421 cggcgaaatg ctgaaaactg cctggcgagg aaacttccgt cgcttgctgg cgcttaaccg
     6481 cgatatcatg ctgcgttcgc tgttgttgca actctgtttc ggcgcgatca ccgtacttgg
     6541 tgcgcgactg gggagtgaca ttatcgctgt taacgcggtt ctgatgacgc tactcacctt
     6601 taccgcctat gcgctggatg gttttgccta cgcggttgaa gcacattccg gtcaggcata
     6661 cggtgcgcgc gacggtagcc agttgctgga tgtctggcgg gcagcgtgcc gccagtcggg
     6721 gatcgtagcg ctactgtttt cggtggttta tttgctggca ggggaacaca tcattgcgtt
     6781 actgacgtcg ttaacccaga ttcaggagct ggccgaccgc tatcttatct ggcaggtgat
     6841 tttgccgttg gttggagtct ggtgttatct gctggacggc atgtttatag gcgcaacgcg
     6901 tgccgccgaa atgcgtaaca gtatggcggt ggccgccgca ggttttgcgc tgacgctcct
     6961 tacgctgccg tggctgggta atcatggttt gtggctggca ttaaccgtct ttctggcgtt
     7021 gcgcgggctt tctctggcgg ctatctggcg gcgtcactgg cgcaacggta cctggtttgc
     7081 cgcaacgtga cggttaaaaa ttctgaataa ataatcctaa gccaaattgc tgactacact
     7141 taatctcacg ttcagaagaa aagtgaacgt actctcattc acaacctaac gatgaggtct
     7201 tgattatgaa taaagatgaa gccggcggta actggaaaca gtttaaaggt aaagtgaaag
     7261 agcaatgggg caaactgacc gatgatgata tgacgatcat tgaaggtaaa cgtgatcaac
     7321 tggtcggtaa aatccaggaa cgttatggtt atcagaaaga tcaggcagaa aaagaggtcg
     7381 tggattggga aacccgcaat gaatatcgct ggtaattaat ccctcctgcc cgacgtgtac
     7441 aaggatgtac gccctcttaa cgcggtttct ttttcacctg cacagagtga tcatgctggc
     7501 actgttcagg atgacgacac gcttccactt ccacgcatgc cgcacagagc ccatgtgctt
     7561 caatcacatt atgccgcagg gcaaaaccca tttttgccgc cagcgtatgc atgatgtctt
     7621 ccacgccttc agcacactct tctttcaccg cgccgcagcg atcgcaaata aacatggctg
     7681 acgtatgggt gggctggtcg aacagatgac agagcacata actgttggtg gattccacct
     7741 tatgcacaaa accttgctca agcagaaaat ccagcgcgcg ataaaccgtt ggcggcttgg
     7801 cttgcggttc agcttcgcgc agtaaatcaa gcagatcata agcgctgata gcgccatcct
     7861 gcagactcat caggcgcaac acttccaggc gctgtggggt caggcgcaca ttacgctgcg
     7921 cgcagatttt ttcagcctgc gctaataact cctgcgttgt ggtcttttcc atcgggcacc
     7981 tcaaagtggg ggttaagaat gtccttactt taccatgttc caggaaaaac accgagaacc
     8041 cttattgttg ccgtaatgtt gattttctgt tttgtaggta aggtgttgtg ttgccttgtc
     8101 gtaccattat caacacgata ataattaata attcattttt aaataaattg cttcaagtta
     8161 actcgctatt tatttaactg ggtttaaacg catacctgta gttcagaaat tatgagcgat
     8221 ttttgatagt gaccagatgg caaaagaatt ataaggagga cacggtacga caaaaaaggt
     8281 agatgaatca tgttgaagat aatacccggt gcaactagat actttaataa aacactcaa
//
LOCUS       DAJOSY010000154         8176 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJOSY010000154 DAJOSY010000000
VERSION     DAJOSY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.154"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            609..827
                     /locus_tag="OHN27_003993"
     CDS             609..827
                     /locus_tag="OHN27_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCQ2288339.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            1045..1287
                     /locus_tag="OHN27_003994"
     CDS             1045..1287
                     /locus_tag="OHN27_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288340.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1617..2546)
                     /gene="fdhE"
                     /locus_tag="OHN27_003995"
     CDS             complement(1617..2546)
                     /gene="fdhE"
                     /locus_tag="OHN27_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCQ2288341.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2543..3178)
                     /gene="fdoI"
                     /locus_tag="OHN27_003996"
     CDS             complement(2543..3178)
                     /gene="fdoI"
                     /locus_tag="OHN27_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCQ2288342.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(3175..4077)
                     /gene="fdoH"
                     /locus_tag="OHN27_003997"
     CDS             complement(3175..4077)
                     /gene="fdoH"
                     /locus_tag="OHN27_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCQ2288343.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(4090..7140)
                     /gene="fdnG"
                     /locus_tag="OHN27_003998"
     CDS             complement(4090..7140)
                     /gene="fdnG"
                     /locus_tag="OHN27_003998"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6553..6555),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCQ2288344.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7334..8167
                     /gene="fdhD"
                     /locus_tag="OHN27_003999"
     CDS             7334..8167
                     /gene="fdhD"
                     /locus_tag="OHN27_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCQ2288345.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1924 a         2072 c         2138 g         2042 t
ORIGIN      
        1 ctctggtgcc atacttttct cataaatatt tttaaaatct atagctaaca aatattatgc
       61 aaagataatg gactagcatc attgtcagta tcattcattt actgctctgt atgttatgaa
      121 taattatgtt gtatgtttgc tcttaaaccg tgggaaggag gctataatgg ttgccaatat
      181 taatctcatt aaacaagaaa gttattctgt tgttaattta gaaaaacaac tttcccgaac
      241 tgttaccaac aaaattataa cgatgagcaa gtaaagaccg gttggtggca gtactgaccg
      301 gtctgtgatg aaagattaat ttcaccttaa atcctggtgc tgttaggatg ctgacgtttt
      361 tcggctacac cattcagatt atgcaaataa atatctctga atataatata ttcatagcac
      421 ccataattca tcctgggcga taactaaacc atttttgagt aataaatgaa acaactgcaa
      481 cactcttatt atttctggaa aacgcctcgc aaaaacaaaa caacatgcaa aaatccgctt
      541 tttttaaatg attttccggc gtaattttcg cacatacatt aatgagtaat atatgtaagg
      601 tattggtaat ggcgatgaat acggtttttc ttcatttatc agaagaggca attaaacggc
      661 tgaacaagct tcggggctgg cgtaaggttt cacgatctgc aattttacgc gaagcggtag
      721 agcaatatct ggaacgtcag caattcccgg tgcgtaaagc aaagggaggc aggcaaaagg
      781 gggaggtagt tggtgttgat gatcagtgta aggagcataa ggaatgacaa aaatttttca
      841 ttcttgaata taaaaaacag atgcccttat tctggtatta atacaaggct gttttacttg
      901 aacttataat aactgcaact gttacatcat atctggaaaa cgcctctcaa aaataaaaaa
      961 tgatgcgtaa atgagctttc tttaaagatt cggcaagggt acttttctct ccatagattc
     1021 aaaaatgata ttggcgagat atttatgaac tctctggcag gtatagatat gggcagaatt
     1081 ttactcgatt tatcgaatga ggtgattaag caacttgatg atctcgaggt gcagcgtaat
     1141 cttcctcgtg cggacctatt aagggaagcg gtagatcaat acctgataaa tcaatcgcaa
     1201 acagcaagaa ccagtgttcc tggcatctgg caagggtgtg aggaagatgg tgtcgaatat
     1261 cagcgtaagc tgcgcgagga atggtaatgg gaaaaaatga tgtcaatcag attgctgaca
     1321 acgtgcgcgt tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaagtcgta
     1381 taaattcaat gtattgcagt ttattggtag gcctgataag cgcagcgcat caggcaatct
     1441 tacgcttgtc atcagtctca tgcccataac cttattgatg tggtggtggg taaattgagg
     1501 ccaaaccggc actggcggca tagcttgagg tggtaacgcg gaatacgaga gatggtgtcg
     1561 tcacacccta tgagctgtaa tccgggcgac ctgcgccacc cggaaaacgt cagagattac
     1621 tctccttcac ccggaaacag gaacgggttg atggaactgc gggcatagcc ttcttgctcc
     1681 attcgtgcgt ccagtaccag agaggcgagg tcatctgcta cggcttcgat ttttggatct
     1741 ttttcctgat agagaatttt caggtaagtg ccgcagtcat cgcagctttc ggctttaatc
     1801 gcggcctgtt cgtcatccag cgaccagtaa tgcagtttgc cgctctgctc gcagttgctg
     1861 cattttacgc gcactacgtg ccattcagtt tcacacaggt tgcagtgcag gtaacgtagc
     1921 ccctgagtgg tgccaatttg caccatgctg gacaccggca tagaaccaca aaccgggcaa
     1981 tattgacgtt gctcgccgta ttcagcgcgg gctttgccgg ggatcagatt ggccatctgc
     2041 gcccagtaga gcgacagtgc agcccagata aacggcgctt tatcgctgct gacggacgag
     2101 aaatcagagg caaacagcgc gctggccata tcttccagct cctgagtcga tgccttctcc
     2161 agattctcaa tcactgccag cgccgggccg ctcatttcag gtttcagctc agcaatcagc
     2221 gccatcagca gcttttgcca gtgcttatca cgcggcagaa cgtgaatatc cagcgggggc
     2281 ttgccttgtg cgctggcttc tttaatgcgc gcagtcagat ccatctccag cggatggtcg
     2341 tacagcacca cttcctgggc gtgggcgata agcgcagcaa agcgcaggta atcacccaga
     2401 ggattatttt ctgccagctc gcgcagacgc tcggcgcggc ggttgtataa attcttgagc
     2461 cgagggaaca ataacggcgg aatcatatcc gccgtacgtt tctcgcttga acccagctca
     2521 tcttgcggga ttatgcgaat actcattcag ctttcttttc cgttgtcttg cggacctcac
     2581 ggtaccagcg cgggtgatgt ttcttcgccc aggcgctggt aacccatcct tccaccatgg
     2641 cggtaatcgt gcctttcacc caaagggcgg cgtagatatg caccatgata accacaatta
     2701 acgccactgc ggcaaatgaa tgcagcatta acgcgaatcg gatcaccggg attgagaaag
     2761 caggcgcaaa ataaggacgc cagataatca cgccgctcac cagcagcaga accaggaaaa
     2821 taatcgccgc ccagaaaacg catttctgac cgaagttata acgcccggtg tcacctactt
     2881 cctcgttgac gacgatctta cgaatattct tcgcccaaaa gatatcatcc cgattgatta
     2941 ggttgtgatg ccagtaacgg aaaaacatga tgatgaacga ggcaaacata accacgccga
     3001 caaacgggtg cagaattcgc gccagctgcg gtgtgcccat gatttgcatc aaccagttga
     3061 aggacgggaa caaaaagccc agcccgctca ccgccgccag gatgaagcag aaggcggtga
     3121 tccagtggtt gatacgttcc ggcgcggtgt agcgcacgat ggtgtcacgt cgtttcattt
     3181 gcgctcctcg tctttctctt cgtgcagatt attctcttcc tcatccgcac ggttcggacc
     3241 gacacccacg tagtggaaga tactggccgc aaaggtagcc gcaaagccaa cagccgcgag
     3301 cggtttccag atgcctttcc agaatttcac ggtttcgctg atttccgggt tctccggcaa
     3361 gccatgatac agatttggct tgtcagcatg gtgcagcacg tacatgacgt gtgtaccacc
     3421 gacgcctgcc ggatcgtaca gacccgcatt gtcgtaaccg cgggttttca gctcagcaac
     3481 gcgctcgctc gccagcgttt tcatcgactc tttcgtaccg aagtgaatcg cgcccgttgg
     3541 gcaggtcttc acgcaggctg gttcttgccc aaccaccacg cggtcaacgc acagcgtaca
     3601 tttgtagacg cggttgtctt ccgggttgag gcgcggaatg tcgaacggac agcccgcaat
     3661 gcaataaccg cagccaatgc actgctcgga ctggaagtcg acgataccgt tggcatactg
     3721 aataattgca ccttccgccg ggcacgcttt caggcagcct ggatcggaac agtgcataca
     3781 gccgtctttg cggatcagcc attccagttt gtcgttctgc tccacttccg agaagcgcat
     3841 taccgtccac gatttggcgc ttaaatcatt ggggttgtcg tacaccccaa tgttattgcc
     3901 gacggtatcg cgaatgtcgt tccactctga acacgccacc tgacaggctt tacagccgat
     3961 acaggtggta acgtcgatga gtttcgccac ttcttcctgg aagtcccgcg cctgaggcgc
     4021 gggggtcaga ccgttagtcg cggaacgacg aatgatatct tgagattgat aagccatatg
     4081 tcgtctccgt tacacctttt ccacattcac aaggaaggac ttaaactccg gcgtctgcgt
     4141 gttcgcatca ccaacgaatg gcgttaaagt attggcaata aagccttttt tagctacgcc
     4201 ttcatagccc cagtgaatcg gaataccgat ggtatcgata tctttgccgt tcgctttcag
     4261 cgtgcgaata cgtttggtca ccaccgcttt ggctttgata tagccacggt tggaggagac
     4321 tttcacggta tcgccctggg caatgccaag tttattcgcc agcgactccc cgatttccac
     4381 aaactgctct ggctgcaaaa tcgcgttcaa cagcgcgtgt ttggtccagt agtggaagtg
     4441 ctcggtcaga cgataggtgg ttccgacata cgggaactta tcggctttac ccaatgcttc
     4501 ggcgtcgtct ttaaagatac gtgcagccgg gttcgagata acgtttggat gcagcgggtt
     4561 agttcccagc ggcgtttcaa acggctcgta gtgttccggg aacggacctt ccgccatctt
     4621 atcgagggca aacagacgcc ccatgccttc ctgctgcatg ataaacggcc cgacgccgct
     4681 acccggaggc gctgcgctgt agtccggaat atcccagccg gtccacttag tgccgtccca
     4741 tttcagcaac tgacgcttcg gatcccacgg gttaccctgc ggatctgcgg aggcgcggtt
     4801 atacagaatg cggcggttaa gcggccatgc ccatgcccag cccagcgtgt taccgaggcc
     4861 agacggatcg gcgttatcac ggcgtgccat ctggttgcct tccggcgtcc agctaccggc
     4921 gaaaatccag cagccacagg aggttgtacc gtcatcgcgc aattgggcga acgaactaag
     4981 ttgttggcct ttcttgacga taaccgcacc ggttgccgga tcggtaatat cggccagcgc
     5041 cttaccgttg ctctccatcg ccacttcttc tgaagatggc tcatgcggaa tggcgtagtt
     5101 ccatgtcatg ttcagcacct ggtccgggtt cgcgccaccc tgttcggcat acatcttgcg
     5161 caagcgcagg aagataccgg agaggatctc gccgtcagtc agcgcaatcc ccggggcgtc
     5221 cgcacctttc cagtgccact gtaaccagcg acctgagtta acgatagaac cgttctcttc
     5281 cgcgaagcag gtcgatggca gacggaacac ttcggtctgg atcttcgacg agtcaacttc
     5341 gttcagctca ccgtggttct gccagaagtt agaggtttca gtgttcagtg ggtcgatggt
     5401 gacgaggaac ttcagtttcg acagacaacc gatcactttg tttttgttcg ggaatgaggc
     5461 aacagggtta aagccctggc agatatagcc attgaccttg ccctctttca tcatctcgaa
     5521 gtattgcagg acgtcgtagc ctttatccca cttcggcaac cagtcaaagc cccagctatt
     5581 ttccgccgtc gctttatcac caaagaaggc cttcatcata gagacgaaga atttcgggta
     5641 gttgccccag tagtttacct ggccttccag cagcggtttt ggcgtgttgg cggtaaggta
     5701 ggtttgcaga tcggtctgct tctcgcttgg cagcgtcatg taacctggca ggctctgcga
     5761 cagcagcccc aggtccgtca gcccctgaat attggagtga ccgcgcaggg cgttaacgcc
     5821 gccgcctgcc atccccatat tgccgagcag cagctggatc atcgccatcg tacgaatgtt
     5881 ttgcgcacca acggagtgtt gcgtccagcc gagggcatac aggaacgagg cagttttatc
     5941 gtgagcactg gtttctgcga tgtattcgca gactttcagg aacgcgtctt ttggcgtacc
     6001 acagatgttt tcaaccacat ctggcgtgta gcgggaaacg tgctgtttca gcaagttcca
     6061 cacgcagcgc ggatgttgca gcgtggtatc gcgtttggcg aagccgtttt cgtccagttc
     6121 ataagtccag gaggatttat cgtacttgcg tttttccgcg tcgtagccgg tgaacaggcc
     6181 atcttcaaag ccgtaatcct cacgcacgat caggctggcg ttggtatagg cttcggtgta
     6241 ttcgcggttg aatttttcat tgttcagcag gtacagcaat acgcctgaca ggaaagcaat
     6301 gtcagtaccg gaacgaatag gggcatagta gtcagctacc gccgccgtac gcgtaaagcg
     6361 aggatcgatc acaatcagct tcgcgccgtt gtgaattttg gcttccatcg cccagcggaa
     6421 cccgaccggg tgagcttcag cggcgttacc gcccatcacc acgacgaggt tggcgttctt
     6481 gatgtcgacc cagtggttgg tcatcgcacc gcgaccaaat gttggagcaa gacttgctac
     6541 cgttggtccg tgtcagacac gcgcctggtt gtcgaccgcg agcataccca gcgcgcggga
     6601 gaatttttgc gttaaatagc cggtttcgtt actcgacgcg gaagcacaca gcatcccggt
     6661 ggagagccag cggttaacag tcacgccttc ggcgttttgc gcaatgtagt tagcatcgcg
     6721 gtcttctttc atcagtttgg cgatgcgatc aaacgcctct tcccaactga tttgctgcca
     6781 tttatcagaa cctggcgcac ggtattccgg aaacttcaga cggctttcgg agtggatgaa
     6841 atccaccagg ccagcgcctt tcggacaaag tgcaccgcgg ttgaccgggt gatccggatc
     6901 gccttcgata tggaagatag atgctttggc gttttttgct ccgtcaccga ggctgtacat
     6961 caacagccca cagcctacgg aacaataggt gcaggtatta cgggtttcgc gggtgcgcag
     7021 cagtttatac tgccgggttt ccgcgagtgc tacgctgggt gcaaaaccca gtgccgctgc
     7081 cgtggtgcct gccataccgc cagcgcagat cttaaagaac tgccttctgc tgacctgcat
     7141 ggattgctcc ttgtttcgac attgtcacgt cccatttaca ttcgcttgct gcgtgtgcag
     7201 ggagtgggag ttatttttct ttgcggaagg ggccgcaaag gtccagaatt ggctcaattt
     7261 ccctccatcc aggaagggtt tgtaacagaa taccataatg ttggtgtgtg tgttcttatc
     7321 tggttaagag aaagtgaaaa aaacacagcg aaaagaaatc gaaaatgtga caaatatcac
     7381 aggtgttcgt caaattgagt tatggcggcg tgatgattta caacacccac ggttagatga
     7441 ggtcgcggaa gaagttcccg ttgcgctggt ctacaacggc atttcgcatg tggtgatgat
     7501 ggcgtcgccc aaagaccttg agtactttgc gctcggtttt tcgctttccg aggggattat
     7561 cgaaagtccg cgcgatatct tcggcatgga tgtcgttcct tcctgtaatg gtcttgaagt
     7621 acaaattgag ctttccagcc gccgctttat ggggttgaag gagcgccgcc gggcgctggc
     7681 gggacgtacg ggctgtggcg tatgcggcgt ggagcaactt aatgacatcg gaaaaccggt
     7741 gcagccgcta ccgttcaccc agacgtttga tctcaacaaa ctggatgatg cattacgtca
     7801 tctcaacgat tttcagccag tggggcaact gactggttgt actcacgccg ctgcctggat
     7861 gttgccatct ggcgaactgg tcggcgggca tgaagacgtg ggtcgccatg tggcgctgga
     7921 caaactgtta ggccgccggt cacaagaagg ggaaagctgg cagcaaggtg cggtactggt
     7981 ttccagtcgt gccagttatg aaatggtgca aaagtcggcg atgtgcggcg tagagatttt
     8041 gtttgcggtg tctgccgcga ccacgcttgc tgtagaagtg gctgagcgct gtaatctgac
     8101 actggtaggt ttttgtaaac cgggtcgggc aacggtttat acccatccgc agcgtttgag
     8161 caattaatta aaataa
//
LOCUS       DAJOSY010000155         8042 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJOSY010000155 DAJOSY010000000
VERSION     DAJOSY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.155"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..982)
                     /gene="pyrD"
                     /locus_tag="OHN27_004000"
     CDS             complement(<1..982)
                     /gene="pyrD"
                     /locus_tag="OHN27_004000"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCQ2288346.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPP"
     gene            complement(1093..1830)
                     /locus_tag="OHN27_004001"
     CDS             complement(1093..1830)
                     /locus_tag="OHN27_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCQ2288347.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1796..2311)
                     /locus_tag="OHN27_004002"
     CDS             complement(1796..2311)
                     /locus_tag="OHN27_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2288348.1"
                     /translation="MLKRIIWILFLLGLTWGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2319..2861)
                     /locus_tag="OHN27_004003"
     CDS             complement(2319..2861)
                     /locus_tag="OHN27_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2288349.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2873..3943)
                     /locus_tag="OHN27_004004"
     CDS             complement(2873..3943)
                     /locus_tag="OHN27_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2288350.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEANTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLYECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3934..6534)
                     /locus_tag="OHN27_004005"
     CDS             complement(3934..6534)
                     /locus_tag="OHN27_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCQ2288351.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNHLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGISWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHTTQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGVQANSGIIDDDGSLYMAGLPAKGTISVRWGEAPDQICHINYELTEQQINS
                     AITRMDAICR"
     gene            complement(6559..7260)
                     /locus_tag="OHN27_004006"
     CDS             complement(6559..7260)
                     /locus_tag="OHN27_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCQ2288352.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKRKEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(7343..7882)
                     /locus_tag="OHN27_004007"
     CDS             complement(7343..7882)
                     /locus_tag="OHN27_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2288353.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQIDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAITPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
BASE COUNT         2085 a         2037 c         1747 g         2173 t
ORIGIN      
        1 gcggcggacc tttaaaaata aaaccagaat aaatttgcac cagtgaggca cccgcagcaa
       61 tcttttcacg cgcagcgata accgagtcga tgccgccaac accgatgatc ggtaagcgac
      121 cgtttaattc cagtgacaag cggcgaataa tttcggtgct ttttaactga agcggacgac
      181 cacttaagcc accggtttga tcgcaatttt tcattccctg aacaagagaa cgatcgagtg
      241 tggtattggt tgcaataacg ccatcaatat tatggcgaac taaactatcg gcaacctgga
      301 tcaattcttc ttcagaaaga tccggcgcga tcttcactgc gatcggcaca tatttatggt
      361 gcatcgcttg caaatcattt tgcttatttt taatcgcggt taagagatca tccagcgctt
      421 caccatattg cagcgtgcgt aatcctgggg tattcggcga tgaaatattg atggcgatat
      481 atcccgcata ggcatagatt ttttccatac aaatcagata gtcatctttg ccctgctcca
      541 ctggcgtatc tttatttttg ccgatgttaa tacccaggac gccgtcataa tgggcctttt
      601 ttacgttctc tacgaggtta tcaacgccaa gattattaaa gcccatacgg ttgatcaaac
      661 cttcggcatc taccagacga aagagacgcg gcttgtcatt acctggctgt ggacgtggcg
      721 tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc taacgcgtca atgcactccc
      781 cgtctttatc aagaccggct gccagaccaa gcggattttt aaacgtcagg cccatgcagt
      841 taacaggttt cgcaggcact ttctgccgca ccagtgcttc aaacggcgtt cctgtaatac
      901 ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg atcgagctgg aaaagggctt
      961 tacgaacgaa ggggtagtac atgaactctc ctggattccc ggtgtgcaaa ccgggggcgt
     1021 attatgtgcg agttcggacc aaaagggaat tgacctgcgg caaaaaaaac gcaaacgttt
     1081 tccgaacctg tttcagggca gattttgctc cttctttgca ctcaacccgc cgtaatcatt
     1141 tatcgcccgc caggcgacat gacacggtgc gattgcctga caccagcttg tctgacgctg
     1201 gcttttaggc ggcacaactc ccacattact taacgcttta ccatttacac tcacctgaat
     1261 caggttaatg taataaggcg ttgggttagt gagttgtaca ccctgcgaat tccgtgtcca
     1321 gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa ccttcgggtc gccagaacag
     1381 tttaaatacc gagcgcatcg ccacttttac gctctgattt tcaactttgc cggatggcac
     1441 gctggcaatg cttaactcaa atagcgtttc gcgatccaca ggcaagatgt cgctttccgt
     1501 tctcagcaag cgcaacgtgc ctgtcgtacc gggcttcagg agaataagtg gcggagcggc
     1561 cagtaatggt gctggcactg tcgacgcttc ccccccggcc caacgcgttg ggcggttgat
     1621 tttactgtta ataagccagg attcctgact ggtattagtc agtaaaatag atatcgattc
     1681 tctgtctgcc ggaaagataa atcgcgttcc gccgaccacc actcctgcct gcaattcgcc
     1741 cgccaccagc agcgataaaa cggcgaaaat caagagcgcc aatctgttcc acgtattagt
     1801 cataatgaag tacaaatgtc gcgctggcat tcactttacc cgactgcaca tcgctattga
     1861 caggtcgcaa ttgcgcataa aaagttaatg gcaccttacc gctatgtagg ctgtagtctt
     1921 tactcgcctg gttaagcgga atcaatatct tcgcatcatc cagaatcgca attgccagcc
     1981 cgctggctgc atccgtacca ctttcaatgg acaacatttc gctctgcact ccgtcggggg
     2041 ttcctgaaaa ggtgacgtca accgtgctga catctgttcc gcaatcctgc aaattaatga
     2101 tgaaagattt ctccgggcca taattcccat gagaaaaacg tgtcagcgag acatcgccta
     2161 actgcacatt gatttgcttg ctatcctgtg ccagcacgca tgtattacgg cgaaacgatc
     2221 cgctaatgtt gaccgtgcca tcatgggcaa atagctcaca gccccacgtt aatcccaata
     2281 agaacagaat ccagattatc cgtttcaaca tcactccttc actggtaaac cagatcaaaa
     2341 tagagaaccg ccgtcgcatt accgcccgtt gctcccgcct ttgtggactt ataacgaagt
     2401 tgatatttga gtgtgttatc cccggctttt aagggcgtaa gaggctggac ctgattgagc
     2461 gggatttctt gctgactttg cgcatcaaga atttgcacgg caatccccgt tgccatacca
     2521 ccgcttccgc ctgtgtcagt tagtttgagc agtgtcggcg caagggtgtc agcctcgccg
     2581 ctaaaaagga cgtccgcgcc agtaatacca gcggcacaac cggtgatatc gatattcaaa
     2641 tcagcagcag tgcttacggt attcgcggcc tgaaaatcac tgatattaaa atcaccaata
     2701 tgaatatttt gtaatgcact tttggtggcg acatcacagc cctgatcgac cacattccca
     2761 taaatatgca tactaacttg ttcaggccca gccatcccga catggatgtt aagaatgcta
     2821 aagaacaaga taacgcactg aaatatcgtt tttttcttca tacgttcacg cgttaatcaa
     2881 aatttattct caccgttgcc gtggcttcaa atcgcccggt ttctggtggt acacctgtcg
     2941 tgctgactgg atagacattc attcgcgcga tactgtctgc atcatcagga atagagagcg
     3001 gtaatgttcc accagcaggg gaaataatgt tgttctggct atcggtcacc accacgccca
     3061 cgccaggacg tgacgtctta atcgcctggg gatagctggg gtctgtggtt gccgtcagac
     3121 tcaggttgac attaactaat cccgcgtttc ccgtgttaca cctgactgga attgcaagtt
     3181 ctgccgttct ggccccaaga ggtttatgcc ctacaacacg gaaattggca gcgaagacat
     3241 cacctaaatc aacttcaatt gtctctccgg cattgacgga acatgagcca agtgaattga
     3301 tcgtaccaga caggtaatac actaaaaccg gtgtacctgt agtcgtgcag gaatcacctg
     3361 cgggtatgtt gtagcattca tataatcgcg ccagcgccat aggaggaatg acgaccgagc
     3421 caacgaacgg ctgcaatatt ttcagggaaa agctaccgct attaccgctt gccccctgcc
     3481 cacctaaacg ttgaccgcca gggtcgcaaa tatgccatcg ggtatccccc cctgtgccca
     3541 cgttttcgaa aggaaccgca gtaggagaac tgcctttaac ctgcaataaa actttggcgg
     3601 caagatagtc attaattttg taccagttgt cgcctaaata gactaacggt gaatccgcgg
     3661 cgaatgccca tcggccactt ctgacattgt ctttatcgca atcacaggaa acataataat
     3721 cattcgattc gttccactga taaaatttgt ctttaactac ccctgccgta tttgcttctt
     3781 cctccagggt tttaacgcca aaattgacgc caatatacgg attcccctga gtagtatgac
     3841 actgcccgct ataagcagcg gtttgagcgc accatagcat taagacggcg gcaaaaaata
     3901 gtcgtagtaa tgacacgcat ttttctccaa agattatctg catatggcat ccattcgcgt
     3961 aatcgcagag ttaatttgtt gttcggtaag ctcgtaattg atatgacaaa tttgatcggg
     4021 agcttcgccc cagcgcacgc ttattgttcc cttcgccggt aaacccgcca tatagagcga
     4081 gccatcatca tcaattatgc cgctattagc ctgtacgccg tccagcgtcg ctatcgcgcc
     4141 aaaacgcagc ggtataccct gctttgatgt tttcattaat acccttgcac ccgcatgggc
     4201 attgaactcc gccagaacca acgccccttt ggtgggaacc acgttgacta ctgcattttc
     4261 aagatccacg ttacgtttta acgacgccgc atcaagtgcg attcgatttt cccgataaga
     4321 agtggcataa ggaacaacgg tatatccgcg ccagtcagtt ttcagcccgg tgctgttcgc
     4381 cacacgagta ttctctgcgc ctggtgctgc aatcagaacg ttagtttccc ccagcgattg
     4441 ccccagggta atgccctgtg aatgggcaac taaactgcct gaaagagcgt agttcagttg
     4501 ttgttgactg ccgttatcgc tgtagttgta gcccactcgg gcatccgcaa acgccccttt
     4561 gtaatccatg ctggcgctac cattagccgt tttcccctcg ctgttatatc cctgttgcac
     4621 gctgtagctc aggttaccgt catcgagtaa tgtctccgtt aaacccaggt tttgtgtagt
     4681 gtgcccttcg ttatcgatac tggtgttaga ggtcatccag gcgtaatttt ttttcctggt
     4741 atagctatca ttacttctgg ggagcaacag attgagcggc agggaaaaat ttagagcaaa
     4801 cacctgatca gcatcaggtt gaccacggga cttactgtag ttccaggaaa tccccagcga
     4861 aagatcttta atttgcgtgt tgtagccaaa ctgtaatagc cgatcctgtt gatccgtatg
     4921 ccagtaagtt tgctggctac cacttaaata aaacgaaccg tactcgccta attgctggga
     4981 gatattgacc tgcagtttgc cacgtttggt gtaaaacaaa ttgtaataac gcgaccacat
     5041 cggcgtatct tcatcatcac cgtcattaaa ttcgtagcca tccatatgtt tatacatggt
     5101 gtcggaaagg gtgtagaaac ccgacgtcga atagcgatag cccatcaatt gaaagttagt
     5161 gcccaagtgg ttgagcgttt tggcatacag aaaacgatat gactgacctt tatgttcgct
     5221 ttgatctgct agcgtactct tcgcctgggt cgcatcgaac gaaattgctc cgaaatcgcc
     5281 gaggttaaat cccagaccaa acatggcggc acggtaatat tcagcatatt gtccaccacc
     5341 ataccaggtc gttccccacg gtccgcccca ctgcaacgtg gcctgggcaa atttgctctc
     5401 ctgctgttca ttactattgg ttctgtattt cgccagtgtc accgcgtatt tgattcgccc
     5461 ctgacgctgg agtaatggca cactggagaa cggtacgctg tagctattga cgctgccgtc
     5521 cgcttctttg atttcaacta acaaatcacc gctcgacgac gtggaataga gatcactaat
     5581 ttcaaaagca ccaggcgata cataagtttg gtaaatggtg taaccatttt gcttaatcgt
     5641 tatctgggca ttgcttttgg cgataccacg caccacaggc gcaaaccctt tcaggctgtc
     5701 tggcagcatg ttgtcatctg acgccagctg cacaccacga aagctgacac tatcgaaaaa
     5761 atcgcctgca gtataatcat cacctacggt cagttcgcct ttcagaggaa taaccgcccg
     5821 ttgcaaatag ctgctgagat tcttccattc tgcggtttgg ccactactac gactccatgt
     5881 tgaattgttg cgcaatctcc atgggcctaa attaacgcca ctgtttaaat tcagaaaata
     5941 gctgtcgcca gaatcactgt ctgcgctgct atgaatactg ttagccccgc taaacgaata
     6001 tcccagtaat agcgcgttga tcccttcatc ccaacgttca ggggggacat aaccacgggc
     6061 attacttttg atggcgattt gcggtactga aatttccaga cggagtttat tcacattaaa
     6121 cgttacgctg gcatccggaa taatctccgc caatggcaca cacgcttgtt tgtccagttg
     6181 tttaaaagcc ggaaacgcat cagtattaac acccattgca tccaggcttt cagtcgtcaa
     6241 acaggcggca atccccttct ctggcgttat ctcaacaaaa ttgacgttgc gcgtatctac
     6301 aattgtctga ttcagaacaa tatcgacacg ataaacccct ggcgttattt tttgtccttt
     6361 ttcgaaacga gacaaatcgg ccaccattga gggatcatct ttgaaaaaag caggatcgaa
     6421 ccaggtttca gcttgtgcat tgaacgctgc cgacacaaac accaccagcc caccaataaa
     6481 cgatggcact ccaccagagc ttaacaggct gtgttgtcga tgagttctat acatctcgct
     6541 ctcttaactg aagacggctt acgatttaca gttgagttgc ggcgtttccg ccccgtagtc
     6601 attgatggtt cgatacgtta cgctgctatt tttggccccc tttggcagag aaaattggta
     6661 tttatcaaag ggggaaatca tttgattttt aactactttt ccacctattt tcaggtctgt
     6721 cagggtaatg aaataaggtg ttgggttact gaccgtaacc atgtcagctg acgaagtgca
     6781 acgcagtttc gccggagcct cgcctaaggg atacgctaaa ccttttggtc gccagaaaag
     6841 tttgatgacg ctttgtgtgg caatcttcag ggtatttccc tctttccgct tcgttgttga
     6901 agggactgcc cgtacgctag tgaagaaaag ggtttctctg tctttcgcca acggcgctcc
     6961 aatgtacata atgcgtaaca gattctcatt tgccggattc agcacaaata gcggtggtgt
     7021 aataataaag tctgctgatt ttttattatc tggggtcatc acccatgact gaataagata
     7081 aggaacatcc ggactggtgt tacgtacacc cagtgaaatt tgctcttcac cttctgaata
     7141 aacaagacgt gttgctgcaa gtccaatccc gcctttacct gccgcccatg cggatgagca
     7201 gctgagtaat attgccgtta aacttacggt cacaattccc tttgttatgc aagttttcat
     7261 aaatagtcac tcttaaaaaa tatgaatatc ccctccagaa cactggaggg gtactaatta
     7321 ataaaacaat taatgagttc tattaataat agttaatatg gaaatttgtt accgcattca
     7381 cattacctga agtcgctttc cccgtagcga tataatcaac tttaaaagtc aacgtctgat
     7441 ctccatcctg caacaaaatg ttcccggttt ctgtgtctgg cgtgatggcc gttgtaccat
     7501 cattcatata catttgcagg gccacgtttg tagcagcatc agatgaggcc tggttagcaa
     7561 acgctgtaat ctgagtagta tccgcagtgc cattaaaggt gaacgttgca ttttttgtta
     7621 cggtagtatc acaatctttt aattcaattg gcacatcaat ttgcacgcca gaaacctgac
     7681 cattggtttg caacttcgtg gcactaacat ctggcaaagt caccacggaa tctttcgtcg
     7741 cagctaccag ggtacaagct ggagcaataa ctttaccatt aaatgttact gagccatccg
     7801 tgatagcatt atcatcagca gccataacgc tggacgttgc acataccaca gttataaacg
     7861 ccgtcaatac actttttttc atcgttatca tcctgatctc ttcgtaaata tgataatgcc
     7921 tttaaatgac attagactta ataataacaa taattgtccg ccatttaaat caagcatact
     7981 aaatccaata agtgacaaat ctttaattta tagatttcat gaatatttaa atattaactg
     8041 aa
//
LOCUS       DAJOSY010000156         7573 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJOSY010000156 DAJOSY010000000
VERSION     DAJOSY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.156"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(145..849)
                     /locus_tag="OHN27_004008"
     CDS             complement(145..849)
                     /locus_tag="OHN27_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCQ2288354.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(986..1438)
                     /gene="moaE"
                     /locus_tag="OHN27_004009"
     CDS             complement(986..1438)
                     /gene="moaE"
                     /locus_tag="OHN27_004009"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCQ2288355.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVNALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1440..1685)
                     /gene="moaD"
                     /locus_tag="OHN27_004010"
     CDS             complement(1440..1685)
                     /gene="moaD"
                     /locus_tag="OHN27_004010"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCQ2288356.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1678..2163)
                     /gene="moaC"
                     /locus_tag="OHN27_004011"
     CDS             complement(1678..2163)
                     /gene="moaC"
                     /locus_tag="OHN27_004011"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCQ2288357.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2166..2678)
                     /gene="moaB"
                     /locus_tag="OHN27_004012"
     CDS             complement(2166..2678)
                     /gene="moaB"
                     /locus_tag="OHN27_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCQ2288358.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2700..3689)
                     /gene="moaA"
                     /locus_tag="OHN27_004013"
     CDS             complement(2700..3689)
                     /gene="moaA"
                     /locus_tag="OHN27_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCQ2288359.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3677..3821)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4086..4994
                     /gene="yvcK"
                     /locus_tag="OHN27_004014"
     CDS             4086..4994
                     /gene="yvcK"
                     /locus_tag="OHN27_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCQ2288360.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5186..7207)
                     /gene="uvrB"
                     /locus_tag="OHN27_004015"
     CDS             complement(5186..7207)
                     /gene="uvrB"
                     /locus_tag="OHN27_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCQ2288361.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1820 a         2010 c         1896 g         1847 t
ORIGIN      
        1 atacaaaata cttttgcatt tatggataca gaaaacgctt ccgctcgtcc ataaggcata
       61 ttttatacct ggtacttgtt aagtgtcgtt tttagtaaga aaaaaatccc gacacatggc
      121 cgggatagtg atggtgaaaa agaattaacg gcggttgccg aagatccgca acaacatcag
      181 gaacaggttg atgaagtcca gatacaaggt taacgcgcca agaatggaat atttgcgcag
      241 gttcgacgtg tcgcgggtat caatctgctc acccatattt ttcagtttct gcgtgtcata
      301 cgccgtcaat ccgacaaaga caatcacgcc gatgtaggta actgcccaca tcaatgcttc
      361 gcttttcaac cagaagttga ccagcgatgc cagcacaatg ccgattaacg ccataaacag
      421 catattgcca aagccactta aatcgcgctt cgtggtgtaa ccgtacaggc tcattgcgcc
      481 gaacatcccg gcagtaacga cgaaagtact ggcgatagaa gcagcggtat agacaatgaa
      541 tatactggaa agcgtaagac ccgtcagcgc cgaataaagc ataaagagca tcgtcgttac
      601 acctgcgctc agcttttgaa tcatcgctga taacacaata accaatgcta attgcgcgat
      661 gatcagaccg attaaaaaga cacggttagt gaacaacagc tccatcacgg ccgcggaatt
      721 agccgcatac caggcaacaa atgcggtcag caacaagcca acggtcatcc agccatagac
      781 ttgagccata taagtttgca agccagcccg gggttgtacg attgaatcag aacgtgggaa
      841 tctgtccatg acgatctcct gaagatataa ggaatatctt aaggatactg caaaatgatg
      901 aggctgtgca tcgacgcagc gtaaacgtat gtactgagcg gtgaaattgc cggacgcagc
      961 ggtgccttat ccggctaaca aaaaactacc agcgttttgc cgcctgctga tcgctctccc
     1021 gagcttcaac ccagcggtcg ccttccggcg tggcttcgcg cttccagaac ggtgcgcggg
     1081 ttttgagata atccataata aactgcccgg cttcaaacgc actgctgcga tgcgcactgg
     1141 tgacaccgac aaaaacgatt tcatcgcccg gccataattc cccgatgcgg tgaatcacag
     1201 tgacgcgccc cagcggccag cggttacgcg cttcatcaac aatttctgcc agtgcttttt
     1261 cagtcatccc cggatagtgt tcgagggtta atgcgttgac gctgtcgccc aggttatggt
     1321 tgcgcacctt accagtaaag gtgactaccg caccgtcttc gtcacgctcc gccagccacg
     1381 ggtactcttc tcctacgctg aacggctgcg gaccaacaac aattttggtt tctgccatct
     1441 taacctccgg ttaccggcgg gaagaaagct acttcgtcgc cgtcagtcag cggatggtca
     1501 aaactcacca gcgtctggtt gacggcagcc agtaatttgc catcttccag cgccagcgcc
     1561 cagcgatcgc tctgcgcagc catgtgctgg cgtaacgctt caacagttgg gaaatccgca
     1621 gccacttcgg ttgcatctgt tcccaccaac tcgcgcacct gggcgaaaaa aagaacttta
     1681 atcatccgct tccaccttaa agtcacccga cttgccgccg ctcttcgcca gcaaacgtac
     1741 cggaccaatc accatatctt tttgcaccgc tttgcacatg tcataaatgg tcagcgccgc
     1801 cacggaggcc gcggttaatg cttccatttc gacaccggtt ttcccggtca ggcggcataa
     1861 ggtttctata cgcacccgat tgtgctccgg ctcggcctgt aaattgactt caactttgct
     1921 gagcatcagc ggatgacaga gcgggatcag atcccaggtg cgttttgccg cctgaatacc
     1981 ggcaatacgc gcagtggcaa atacgtcgcc tttgtggtgg cgaccatcaa taatcatcgc
     2041 cagcgtctcg ctgcgcatgg tgacaaaggc ttccgcccgc gcttcacgca cggtttccgc
     2101 tttggcggag acatccacca tgtgcgcttc gccagcggcg ttgatatggg tcagttgcga
     2161 catacttatt tcttcaaatg tggatggaaa ttacacggac gcgtacgggc atccagctgc
     2221 ggcgcgatga tattttccca tgcggtacgg cacgctttgg tcgaacccgg catggcgaaa
     2281 atcagcgttt tgttggcgac gcccgctacc gcacgagatt gcaacgtgga agtgccaatc
     2341 tcttcaaacg acaacatccg gaacacttca ccaaaacctt caacttcacg gtcgaacaac
     2401 ggcagcaatg cttcgggagc ctgatcacct tccgtcaggc cagtaccacc cgtaatcaat
     2461 accacttgta catcgtcgct ggcgatccac gcagatacct gagcgcgaat agcgtagcgg
     2521 ttttctttca caatggcttt atcgacaacg tgatggcccg cttcttgcgc cgaatcgcgc
     2581 agatagtgac cggaggtatc gtcttcttca ccgcgacgat tagaaaccgt aagaatagca
     2641 atacgggtcg ggataaattc agtgcttacc tgactcatct gatctctcct tttgacgttt
     2701 tagccgccaa tgtacgataa gttttgcgta ataccggtgt tgttttgatg caggaaatgg
     2761 gtctgtttct tctcccgcag cgccgctgaa atacgcgctt ccagcgcctg ttgctgggta
     2821 tcgtcttcca gcagatcgcg caggttaacg ccgccttcac caaacaggca gagatggagt
     2881 ttaccaatgg aggaaacgcg caggcggttg caagtggcgc agaagtcttt ttcatacggc
     2941 atgataaggc caatctctcc ggcgtaatct ggatggcaaa agacttgcgc gggaccgtcg
     3001 ctgcgttgac gtaattggtg gatccagccg cgacgcagta gctcgtcacg cagaacctga
     3061 ccagagatgt gatgcttacg gaagagctcg ctgccctcgc ccgtttccat cagttcgatg
     3121 aaacgcagct ggatagggcg atgctggatc cagttcagaa aggtgtcgag ctggtgatga
     3181 ttaacatcac gcatcagcac ggtattgact ttgaccttct caaaaccggc ctcaaatgca
     3241 gcatcaatcc ctgccatgac ctggttgaat ttatcctgcc cggtaatagc gtgaaactgg
     3301 cgggcgtcca gactgtcgac actgacgtta atgccagtaa gtcccgcatc gcgccagttc
     3361 gccacatcgc gttccagacg gtaaccattg gttgtgaccg caatctggcg gatagcgtcg
     3421 ttttcccgca cagcggcgat gatatcggta aagtcgcggc gtaaagacgg ctctcctcct
     3481 gtcaggcgca ctttttcggt gcccagactg gcgaaggcgc gcgtaacccg gcgaatttca
     3541 tcgacggtaa gaaagccttt attggtgacg ccgctcggtt tgtagccatc cggcaggcag
     3601 taggtgcaac gaaagttaca cacatcggta atcgacaggc gcaagtagta aaacttacgc
     3661 gcaaatgcat cagtcagttg tgaagccatg tacacctttc cagatacggg aggcgaagtc
     3721 atttcttcct tcgccctggt ggcaatcttt acgagcattg tcacggccaa agcaccgtat
     3781 cagttgaccc aggtgcagag gctagagtgt ttagtggtta tgccgatact agcgtgtaaa
     3841 tgagattttt accatccaca ctttcgctat gtaatcatgt atatagcgtc atgatcgtgc
     3901 tatttcgcta cagaataagg gaaaatggcg gttttacttt gatatacatc atcagggagg
     3961 catagcgtgc atcgtccttt agaggtagaa gaaaatagtt tgtatcctca atattggcag
     4021 gttaattgct gtttccccgc aatttgcgct actgtagcgc ggataatctg actccaggaa
     4081 actatatgcg caatcgtacg ctggctgatc ttgatcgtgt cgttgctctc ggcggagggc
     4141 atggactggg acgcgttctc tcatcacttt cgtctttggg ttctcgttta acgggtatcg
     4201 tcaccaccac cgataatggt ggctcgacgg ggcgtattcg ccgttcagaa ggcggcattg
     4261 cctggggcga tatgcgcaac tgcctcaacc agctgataac ggaaccgagc gtcgcctccg
     4321 cgatgtttga ataccgtttt ggtggcaatg gcgaactttc cggtcataat ctcggaaact
     4381 tgatgttaaa ggcgctggat caccttagcg tgcggcctct ggaagccatc aatttaattc
     4441 gtaatctgct gaaagtggat acgcatttga ttccaatgtc agagcatcct gttgatctga
     4501 tggcgattga cgatcagggg catgaagttt acggcgaggt caatatcgac cagttaacta
     4561 cgccgattca agagttattg ttaacgccta atgtacccgc aacgcgtgag gcggttcacg
     4621 ctatcaatga agcggatctc atcattattg ggcctggcag tttttatacc agcctgatgc
     4681 caattctgct gctgaaggaa atcgcccagg cattacgccg cacgccagcg ccgatggttt
     4741 atatcggcaa tctggggcgt gagttgagtt tacctgcggc taatttgaag ctggaaagca
     4801 agctggcaat tatggagcag tatgttggta aaaaggtcat tgatgcggtc atcgtcgggc
     4861 caaaagtgga tgtctcggcg gtgaaagagc ggattgtgat ccaggaggta ctggaggcca
     4921 gcgatattcc gtatcgtcat gaccgccagt tgttacataa cgcgctggaa aaggcgttac
     4981 aggctttagg ttaacggaaa tccgaaggaa aattccggct tcctattgaa gacaaagtgc
     5041 gcgttgttta tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc gcgcaaattc
     5101 aatatattgc ggagaaaatg taggcctgat aagcgtagcg catcaggctg ttttccgttt
     5161 gtcatcagtc ttcttcgcta tcctgttacg atgccgcgat aaacagctca cgcagctgat
     5221 gcaactggtc acgaatttgc gccgcttctt cgaactccag attctgcgcg tgttgcatca
     5281 tcaacccttc cagctcatgg attttctgct gcaacgcttt aggcgacata tccatcggca
     5341 cattatccgg ctcaacaatc gggcgcgatt ttcctctgcc cttcgctttg gttttggcaa
     5401 tgttctgccc cagcgccagg atatcgacca ctttcttgtt caagccttgc ggcgtaattc
     5461 cgtgttcctc gttgtacttc tgctgtttct cacggcgacg ttcggtttcg ccaatcgctt
     5521 tcgccattga tggggtgatc ttatcgccgt agagaatcgc tttaccgtta acgttacgtg
     5581 ccgcacgacc aatggtctgg atcaacgaac gttcggaacg caggaagcct tctttgtcag
     5641 cgtcgaggat cgccaccagc gacacttccg gcatatccag accttcgcgc agtaagttga
     5701 tccctaccag cacgtcgaac tcacccagac gcaagtcgcg gataatctcc atacgttcga
     5761 cggtgtcgat atctgagtga agataacgca cgcgctcgcc atgttcttcg agatattcgg
     5821 taagatcttc cgccatccgc ttggtcagtg tggtgaccag tacgcgttcg ttaattgccg
     5881 ctcgctgacg aatctccgaa agaagatcat caacctgtgt cgccaccggc cgcacttcga
     5941 taatcgggtc aagcaatccg gttggacgca ccacctgatc caccacatcg ccgccggatt
     6001 tttccagctc gtaattaccc ggcgtcgccg aaacatagat ggtttgcggc gctaatgctt
     6061 cgaactcttc aaacttaagc ggacggttat ccagcgctga tggcaggcgg aagccgtact
     6121 ccaccagtgt ctctttacgc gcccggtcac cgcgatacat gccgccaatt tgtggaatgg
     6181 tgacgtgaga ttcatcgacg accagcagcc catcggcagg caggtaatca aacagcgtcg
     6241 gcggtggctc acccggtcca cgaccggaga ggaagcgcga gtagttttca atccccgaac
     6301 agtagcccag ctcgttcatc atctccagat caaactgggt acgctgggtc agccgctgct
     6361 cttccagcag tttgttgttt tccaacagca ctttgcgtct ggcggccagc tcttctttga
     6421 tctcctccat cgcctgtacg atgcgctcgc gcggtgtgac gtagtgcgtt ttcgggtaga
     6481 tggtaaaacg tggaatagtg gaaacaatct gcccggtcag cgggtcaaat aacgacaatc
     6541 gttccacttc ctcgtcaaac agttccacgc gaagtgcaat gtcatccgat tctgccggga
     6601 agatatctat cacctcgcca cgaacgcgga aagtaccacg ctggaatgct tgatcattac
     6661 gagcgtattg cagctccgcc agtcggcgca gaatcgcgcg ctgatcgata atcataccga
     6721 ccgtgagatg gagcatcatc ttgagatata aatcaggatc gcccagacca taaatcgcgg
     6781 aaacagacgc caccacaacc acatcacgcc gctccagcat cgctttggtg gcggacaaac
     6841 gcatctgctc aatatgttcg ttaaccgagg catctttctc aatgaaagtg tcggaactcg
     6901 gtacataggc ttccggctga tagtagtcgt agtaggaaac gaaatattcc accgcgtttt
     6961 ccgggaagaa ctctttcatt tcgccataca gctgggccgc cagcgttttg ttgggcgcaa
     7021 gtaccatggt tgggcgctga aggtcagcaa tgacattggc aatggtgaag gttttccctg
     7081 agccagtcac gccaagtaac gtctggtgcg ccaggccatc ttccagcccc tcttcgagac
     7141 gtcgaatcgc ctctggctga tcgccagaag gtttaaaagc ggaattcagt ttgaacggtt
     7201 tactcatgag tcgctacctg aaggagttgg gcgggcaggt atgtaatttt actcgtcgta
     7261 cttaattatg ccaacaaatt atactggata aaaaaacagt tcatcaccat aatatttctg
     7321 atacagcgta aactccgctg tcaatcatga gcaaaattta ctctgtggcg agataaaact
     7381 ccggccttac cgggttatcc ccaaagcaac ggctttttta acatttgtca agatgagtga
     7441 tgacagtttt atggcaagag atgcctgttc agtgactgcc attgcattta tctaaccagt
     7501 taaaaaataa aagatatttc tttgagccgt ctttaacgcc aattcgcgtg caagccgcgt
     7561 attctctcgc ttg
//
LOCUS       DAJOSY010000157         7377 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJOSY010000157 DAJOSY010000000
VERSION     DAJOSY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.157"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(164..526)
                     /locus_tag="OHN27_004016"
     CDS             complement(164..526)
                     /locus_tag="OHN27_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCQ2288362.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
     gene            811..1020
                     /locus_tag="OHN27_004017"
     CDS             811..1020
                     /locus_tag="OHN27_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288363.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1032..1619)
                     /gene="mtlR"
                     /locus_tag="OHN27_004018"
     CDS             complement(1032..1619)
                     /gene="mtlR"
                     /locus_tag="OHN27_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCQ2288364.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1619..2767)
                     /gene="mtlD"
                     /locus_tag="OHN27_004019"
     CDS             complement(1619..2767)
                     /gene="mtlD"
                     /locus_tag="OHN27_004019"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCQ2288365.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(2997..4910)
                     /gene="mtlA"
                     /locus_tag="OHN27_004020"
     CDS             complement(2997..4910)
                     /gene="mtlA"
                     /locus_tag="OHN27_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCQ2288366.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5447..5809
                     /locus_tag="OHN27_004021"
     CDS             5447..5809
                     /locus_tag="OHN27_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCQ2288367.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5812..6948
                     /locus_tag="OHN27_004022"
     CDS             5812..6948
                     /locus_tag="OHN27_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2288368.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFFAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT         1785 a         1852 c         1847 g         1893 t
ORIGIN      
        1 tatttcggta aagatgcatt ttcagaatat caaccagcgg taaatcgacg taaggggagt
       61 gaattgtttg gtcattaggc gagtagacag gagagtcccc gcattgacca gctaacgcaa
      121 gtcagatgcg ggttgatacg atcgtaccag tcgtctgagg ggcttaaaac gcggtctttg
      181 aaaacccggt catctcttcc aggcccattt cacggcccag tgcggtcatt gggtgcacaa
      241 ccaccagtcc gcgaacactt ttcttcagct tgcccatatc agcctgctct tttttggtaa
      301 ttgcgcgctg aaacggcatc ttcatcagtt tttgtgcttc tttactcagt ttttggctat
      361 gaacctcacg caggcgagca atttctgctt ccagcgtggc tttctctttt tccagctcgg
      421 catatttgtc tgcggcttca accagcgaca gatcggcctg ctggtggcgg atggcgtcca
      481 ggcgatcgct gagacgtttg atttcgtttt tttcgacttc tttcatgtga tggcttctga
      541 taaagataaa tttatagccc ctagtatgcg cgaccaagtg ctttgttcac aactgatgac
      601 attgtaatag ttctgcaacg taatcattaa catgctcaaa aactacgcaa tccgttgatt
      661 aattaagaaa tatatagaaa aaagataata aacagcagcg gatgattatt gaggattcag
      721 cacgttttcc ttaatgtcat ctttaccggg aacctaaact aaaattggcg gccgcgcatt
      781 cccggcgcgg ggtaatacgg agatatcatc atgggcaaat taggtgaaaa cgttccgctt
      841 cttatcgata aagccgtaga tttcatggca tcaagccagg cgttccggga gtatctgaaa
      901 aaacttcctc cccgtaacgc gattccgtcc ggaatacccg atgaaagcgt gccgttatat
      961 ctacaacgtc tggagtatta tcgtcggctt tatcggccga agcaggtaga ggggcagtaa
     1021 gcagacgttg cttacttttg aaaggctttt tttaagctga ttttggaaat cagctcagtc
     1081 agtgaaagga ccattgttga tcgcacagcc tgttgatagc gctgaatttg cattgcatac
     1141 aaactggagt ctgctggttc aaactgtggc ggcggtggta acgccgccac gcagtgcagt
     1201 tcaccaaagg gtccaaggat ttcgtcgtcg gtaaaggcgt actcgttgcc gtcgtgattt
     1261 agctcttcac gcaatgccat cagcagttcc gcatcttcgt attcctggcg gttaatgacg
     1321 cccaacccgt aaatgagttt taaacgcaca gaaagatcgc ccagcggacc atcgccgtcg
     1381 agtaacggtt ctacagcata cttcaccgcg taatcgtctt tgcggaatac ctgaagcacc
     1441 agaagattta ccgcctcggt caggagctcg acggcggtga tcagaaagct tcgcacggtt
     1501 ttgccagcat tcagacgctc aagcacacgg ttttcaaagg cctgggtttg ttccattgtt
     1561 gcctgcatat ctgacaatct gttattcggg cgcagggtgt cctgcgcctg gtccaccatt
     1621 attgcattgc tttataagcg gttaccgcct cggatacaac ctcgctgttg gcatcaagac
     1681 cggaaatctg tgccagcgcc gcctgcggac ctttgtcagc gatcagtgct gccagttcct
     1741 gagcctgcgg atcatcttca ctgcggaagt gcattgcagc ggcaatacct tcaatcaggt
     1801 ttttatgtgg cagaccatat tccagcgtac cgagcagtgg cttgatcaga cggtcgccag
     1861 cactcagttt acgcagtggc tgacggccta cgcgctctac atcatctttc agatacgggt
     1921 tctcaaaacg gccgagaatt ttctggatgt acgccgcatg cttgtcagcg tcaaagccgt
     1981 agcgcttgat caatactgca ccactttctt ccatcgcacc ttttaccacc gcgcggattt
     2041 tctcgtcgag aatcgcgtca cgaatggtct gatgaccggc cagttttccg aggtacgcgg
     2101 ttatagcatg acccgtgttc agggtgaaga gtttacgttc gacaaatgcc atcaggttgt
     2161 cggttaactc catgcctggg atgttcggca gtgcgccttt gaactgcgtt ttatcgacaa
     2221 tccattcgct gaaagtttct accgtcactt ccagcggatc gttagttgcc gaagccgaag
     2281 gcggtacgat gcggtcaacg gcggaatcga caaagccaac gtgttcttct acccacgctt
     2341 tggcgtcttc cggcagggcg ttcatcacat ggcctttcag ctgcgtggta ccgcgtacca
     2401 tgttttcaca ggcgatgatg ttcagcgggg attcattacc ttgttcttta cgtttcacca
     2461 gccctttggc gattgccgga gcaatacgtt ccagcacaac cgggccaacg gcggtagtga
     2521 ctaaatcaac ctgagcaatc agatcaacga catcatcacc aatgctgctg acagcattga
     2581 cgccggaaac ggtatctacc tgctcggttt caccaaccac atgtacctga tagctatgac
     2641 gggcattcag ggcatcaagt accacctgat tgacatcggc aaacgtcagt tggatacccg
     2701 cgtctgccag cagtttaccg ataaagccac gaccgatatt acctgcgcca aaatgtaatg
     2761 ctttcatagt attaaccttc atcaatgttt ttacccgaga gggctggggt gaggcttttc
     2821 cctcacccaa accctctccg tgaggagagg ggcagcgccg gatgcggcgt aagcgcctta
     2881 tccggcctac aagaacgtgc aaattcaaca aattgtgatg catcctgtag gcctgataag
     2941 cgtagcgcat caggcaatta accccacctt ctccatgtgg agagggtggg attggattac
     3001 ttacgacctg ccagcagttc cagcacttca tccacgctgg tggtgtgtgc cagacgctcg
     3061 atgacggact catcatccag tgcattggtc aggctggtga taacctgaat gtgctcgttg
     3121 ttacgggcag caataccaat caccagacgg gcaatgtcat cttcttcttc accgaagcgc
     3181 acgccttccg ggtactggca gaacacgacg cccgttttca gtacgcgatc tttcgcttca
     3241 accgtaccgt gtggcaccgc gatagactca cccagataag tcggggtcag tttttcacga
     3301 tccagcatcg cctgaacgta ttccggctca acgtaaccgc ctttcaccag ctgctcgcca
     3361 gcaaaacgaa tcgcttcttc tttggttgcc gctttgcgac cgaggaagat gttctccgcg
     3421 cctagcttga acaggttagc actggaatcg tcaaagctgt ctttcaggct gtcttttact
     3481 ttctcttcgt ttgccgtgtg gcgttgggca gcaaccagac gttcggtcag gctggtgtac
     3541 aggccgctgt cgaggaagtt ggtcagcgaa atatgctgtg cctgcggaac ctggcgcata
     3601 gcgcgttcgg tcaggtcacg gtgagtgatg acgaggtcca catctggcgg caggttgttg
     3661 atcgcgctgt tagtaacaga aatctgcgac agacctgcat cctgaatttt cttacgcaga
     3721 acgcctgcgc ccatcgcact ggaacccata ccggcgtcac aggcaacgat gattttacgt
     3781 acgtggctca ggtcgttagt cacatcgcca gcagacagcg gagatgcgcc tttagactca
     3841 gctttcatgt cctgcatacg acgagttgct gcttcaatat catcttcttc tttcactttg
     3901 ctggttttca gcaaaatagc agagacaacg aaggagacag ccatcgccgc acacacaccc
     3961 gcgatgttag cgaagtaagc accttttggt gtcatcgcca gtacagcaag gatagaaccc
     4021 ggagatgccg gagaaaccag accaccgccc aggatagtca gcgtgaacac gccagtcata
     4081 ccgccgagga tgactgcgag gatcagacgc ggattcatca gcacatacgg gaagtagatt
     4141 tcgtggatac cacccaggaa gtggatgatt gccgcaccgc ccgcagactg tttagcgcta
     4201 ccacgaccaa agaacatgta cgccagcagc acgcccatac ctggacctgg gttagcttca
     4261 atcaggaaga agattgattt acccagttca tgggactgct gaatacccag cggcgagaag
     4321 ataccgtggt taatggcgtt gttgaggaac aggattttcg ccggttcaac aaagatagac
     4381 gccagcggca gcatgtcatg gacaaccatg aagttaacgc ccgcagccag cattttggac
     4441 agggcttcaa caatcgggcc aatgccgagg aatgccagaa tagcgaggat catcccgatg
     4501 atgcctgcgg agaagttatt caccagcatc tcaaaaccgg atttgatctt accgtctacc
     4561 cagcggtcga agtgcttaat gcaccagccg cccagcggac ctgcaatcat agaaccgagg
     4621 aacatcggca tgtctgcgcc gacgataacg cccatggtgg tgatggcacc gactacgccg
     4681 ccacgttcgc cgcctaccag cttaccaccg gtataaccga tcagcagcgg caggagataa
     4741 gtgatcatcg gcccgaccag cttcgccagc gtctcgttcg gtaaccaccc tgttggaata
     4801 aataacgcgg tgatgatacc ccacgcgata aacgcgccga tatttggcat caccatgttg
     4861 ctgaggaaac gaccaaagct ttgcactttg atcttaatat cggatgacat aaaaacaccc
     4921 cttcttatgt ttgctgtcgc gcaggctgga agcccgaggt tttttgttaa tgtggcggca
     4981 gaggtagccg gaccttgtag atactgcgaa atctggcact gaatcggtta actgtccagt
     5041 cgacggcctt ttgtgtgata tcaatcacat aaatataggg ggtagagagg taattgacgt
     5101 gacgttcatc acaaaacgct tgtgtaaaaa agcaacaagg cggcgatttt cgctaaaaat
     5161 ggcatttaaa tgtgagtagt gtcacatttt tgttttgatg gtttgttgaa tctttgtgat
     5221 ctgaatcaca agatattttc ttacggcgtt cacctgatgt gatctacagc atgttatgcc
     5281 ttcgtcatac aatcgttatg taactaagca actcatcacc tctaaaaatt attctttggt
     5341 gttatggcga gaatcgcgaa cccggattat tgtcaaaata tcagcctgac tcccttctgg
     5401 ctgccagccc aaaaacaaca ctgataaacg tgaatccgag gcagatatgt ttctaaacta
     5461 tttcgcgttg ggagtgctga tcttcgtctt tctggtgatt ttttatggaa tcatcgcgat
     5521 acatgacatc ccttatctga ttgccaaaaa gcgcaaccat ccccatgccg acgctattca
     5581 tacggcgggc tgggtgagcc tgtttactct gcatgttatc tggccgtttc tgtggatctg
     5641 ggcgacgctc tatcaaccgg agcgtggctg gggtatgcag tcacatgttg cgtcgcagga
     5701 gaaagcgact gacccggaaa tcgccgcact ttctgaccga atttcccggc tggagcatca
     5761 actcgccgcc gagaaaaaga ctgactattc cacgttcccg gagatctaat tatggatcta
     5821 ttgattgttt taacttacgt ggcgctggcg tgggcggtct ttaaaatctt tcgtattccg
     5881 gtgaatcagt ggacgctggc gacggcggcg ctgggtggcg tgtttctggt gagtggtttg
     5941 attttgttga tgaactacaa ccacccttac acttttaccg cgcaaaaggc agtgatagcg
     6001 atccccatca cgccacaggt gacgggaatt gttactgaag tcactgacaa gaataatcag
     6061 cttattcaaa agggcgaggt gctttttaag ctcgacccgg ttcgttacca ggcgcgagtt
     6121 gacaggcttc aggctgacct gatgacggcg acgcataata taaagactct gcgcgcgcag
     6181 ctcacagaag cgcaggccaa caccacccag gtttcagcgg agcgcgaccg tctgtttaaa
     6241 aattatcaac gttatctgaa aggcagccag gcggcggtga atccgttctc ggaacgtgac
     6301 atcgacgatg cgcggcaaaa tttcttcgcg caggatgcgc tggtgaaagg ctcggtggcg
     6361 gagcaggcgc agatccagag ccagctcgac agtatggtta acggcgagca atcgcagatt
     6421 gtgagcttaa gagcgcaact tactgaagca aaatataatc ttgagcagac tgtcattcgc
     6481 gcaccaagca atggctacgt cactcaggta ctgatccgcc caggcacata cgcagctgcc
     6541 ttgccgttgc gtccggtgat ggttttcatc cccgagcaaa aacggcaaat tgtcgcccaa
     6601 tttcggcaaa actcgctgtt acgtctgaaa cctggtgatg atgcagaagt ggtgtttaac
     6661 gcgctacctg ggcaggtgtt ccacggcaaa ctgaccagta ttttacctgt cgtgccaggc
     6721 ggttcttatc aggcgcaggg ggtattgcaa tcattaacgg tcgtgcccgg cacggacggt
     6781 gtgctgggaa ccattgaact ggaccctaac gatgatatcg atgccttacc cgacggcatc
     6841 tacgcccagg tggcggttta ctccgaccat ttcagccatg tttcggtgat gcggaaagtg
     6901 ctgctaagaa tgaccagctg gatgcattat ctttatttgg atcattgagg gggagggggg
     6961 agattatgga actttgctcc ttgtaaggac taattatttt tgcatattac ttcattagct
     7021 gcgatgattt ttctttaata ttcattatgt taataagttc tggatgagtg tgctctcatt
     7081 ggatttttta tccgtcttgt tctgccgcag gatgagacta tacatcgtcg agatcgcaat
     7141 gatgatatga gttgttcctc ctgcgacggc tctaatcaaa aaagagcagg agtgcttttc
     7201 catctattgc gatgatggct ttattatctg aaggatgaca gtcacattta aaaaatattt
     7261 aatctataga tcacaatgta gggtcatgga atatatgtat acaactattt tcttcttcac
     7321 ttttaattat cgtattcatt ataaaactaa gttaaaaaaa ctcctggaga gtgagtt
//
LOCUS       DAJOSY010000158         7213 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJOSY010000158 DAJOSY010000000
VERSION     DAJOSY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.158"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1769
                     /gene="traD"
                     /locus_tag="OHN27_004023"
     CDS             <1..1769
                     /gene="traD"
                     /locus_tag="OHN27_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HCQ2288369.1"
                     /translation="GGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLH
                     GTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWD
                     LWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAYLMRNDPNRSYSKLVDT
                     LLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKAIRYLQGIEHNGESFT
                     IRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRVWFF
                     CDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAF
                     FRSPSHKIAEFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQS
                     LPDLTCYVTLPGPYPAVKLSLKYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGR
                     QMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQPQQPQQPQQPQQPVSSAINDKKSDS
                     GVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQQMQR
                     REEVNINVHRERGEDVEPGDDF"
     gene            1769..7039
                     /gene="traI"
                     /locus_tag="OHN27_004024"
     CDS             1769..7039
                     /gene="traI"
                     /locus_tag="OHN27_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCQ2288370.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHT
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARVGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSSVNMLPESERPRIVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVIAVSGDSVTLSDGQ
                     QTRVIHPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGRTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            7059..>7213
                     /locus_tag="OHN27_004025"
     CDS             7059..>7213
                     /locus_tag="OHN27_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCQ2288371.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINL"
BASE COUNT         1662 a         1861 c         2363 g         1327 t
ORIGIN      
        1 caggtggtcg tcagctgaca gacaatccga aagacgttgc ccggatgctg aaaaaagacg
       61 gcaaggactc cgatatacgg attggcgacc tgccgattat ccgggattcc gaaatccaga
      121 acttctgtct gcacggcacg gtcggggccg gtaagtcgga agttatccgt cgtctggcca
      181 actacgcccg tcagcgtgga gatatggtgg tgatttatga ccgttcaggg gaatttgtta
      241 aaagttacta tgatccctcc atcgataaaa tcctgaatcc gctggatgcc cgctgtgccg
      301 cctgggattt gtggaaggag tgcctgacac agccggattt tgataatacc gcgaacacgc
      361 tgatcccgat gggcacaaaa gaggacccgt tctggcaggg ttcaggacgt accatttttg
      421 cggaagcggc gtacctgatg cgtaatgacc ccaaccgcag ctacagcaaa ctggtggaca
      481 cactgctttc catcaaaatc gaaaaactgc gtaccttcct gcgtaattca ccggcggcca
      541 acctggtgga agagaaaatt gagaaaacgg cgatttccat ccgtgctgtg ctgaccaact
      601 acgtgaaagc catccgttac ctgcagggga ttgagcataa cggtgagtct tttaccatcc
      661 gtgactggat gcgtggtgtc cgggaagatc agaaaaacgg ctggctgttt atctcgtcga
      721 atgccgacac ccatgcctcc ctgaagccgg tgatctccat gtggctgtcc attgccattc
      781 gtggtctgct ggcaatggga gaaaaccgta accgtcgtgt gtggtttttc tgtgatgagc
      841 tacccacgtt acacaaactg ccggacctgg tggagatcct gccggaagcc cgtaagttcg
      901 gtggctgtta tgtgtttggt atccagtcct atgcccagct ggaagatatc tacggtgaga
      961 aagcggctgc cacgctgttt gacgtcatga acacccgtgc ctttttccgt tctcccagcc
     1021 ataagattgc agagttcgct gccggtgaga ttggtgagaa agagcacctg aaagccagcg
     1081 agcagtattc ctacggtgct gatccggtac gtgacggggt atcgaccggt aaggatatgg
     1141 agcgccagac gctggtcagt tattccgaca ttcagtctct gccggatctg acctgttatg
     1201 tcaccctgcc cggaccgtat ccggcagtaa aactctctct gaaatatcag acacgaccga
     1261 aggtcgctcc ggagtttatt ccgcgtgaca tcaacccgga aatggagaac cgtctgagtg
     1321 ccgtacttgc cgcaagggaa gcagaaggtc gtcagatggc cagcctcttc gaaccggatg
     1381 tgccggaagt tgtttccgga gaagacgtga ctcaggctga acagccgcaa cagccgcaac
     1441 agccgcaaca gccgcaacag ccgcaacagc cgcaacagcc gcaacagccg gtgtcttctg
     1501 ccatcaacga taagaagtca gattcaggtg tgaatgttcc ggcagggggg atcgagcagg
     1561 agctgaaaat gaaaccggaa gaagagatgg aacagcaact gccacccggg atcagtgaat
     1621 ccggtgaagt ggtggatatg gccgcttatg aggcatggca acaggaaaat catccggaca
     1681 tccagcagca gatgcagcgt cgtgaagagg tgaacattaa tgtgcaccgg gagcgtgggg
     1741 aggatgttga gccgggagat gatttctgat gatgagtatc gcgcaggtca gatcggccgg
     1801 aagtgccggt aactattata ccgacaagga taattactat gtgctgggca gcatgggaga
     1861 acgctgggcc ggcagggggg ctgaacagct gggactgcag ggcagtgtcg ataaggatgt
     1921 ttttacccgt cttctggagg gaaggctgcc ggacggagcg gatctaagcc gcatgcagga
     1981 tggcagtaat aagcatcgtc ccggctacga cctgaccttc tccgccccca aaagtgtctc
     2041 catgatggcc atgttaggtg gcgataagcg cctgattgat gcacataacc aggccgtgga
     2101 ctttgctgtt cgtcaggtgg aggcgctggc ctccacacgg gttatgacgg acggacagtc
     2161 agaaacggtg ctgaccggta atctggtgat ggcactgttt aaccacgaca ccagtcgcga
     2221 tcaggaacca cagctacaca cgcatacggt ggtggctaat gtcacgcagc ataacggcga
     2281 atggaagaca ctgagcagtg acaaagtggg gaaaacgggg ttcattgaga atgtgtacgc
     2341 taatcagatt gcctttggca ggctctaccg ggaaaaactg aaagagcagg ttgaggcgct
     2401 gggctatgaa accgaagtgg tgggtaagca cggtatgtgg gagatgccgg gcgtaccggt
     2461 ggaggccttt tccggacgct cacaggcaat ccgggaggcc gtcggggaag atgcctcgct
     2521 gaaatcccgg gatgtggcgg cactggatac gcgtaaatcc aaacagcacg tcgatccgga
     2581 aatcagaatg gctgaatgga tgcagacgct gaaggaaacc gggttcgaca tccgggcata
     2641 tcgtgacgcg gcggatcagc gtgcggagat ccgcacgcag gcgcccgggc cagcttcaca
     2701 ggacgggccg gatgtgcagc aggcggtgac acaggcgatt gccggattaa gtgaacgaaa
     2761 agtgcagttc acgtatacgg acgtgctggc caggacggtc ggcatactgc cgccggaaaa
     2821 tggtgtgatt gaacgggcac gcgtcggtat cgatgaggcc atcagccgtg agcagcttat
     2881 ccccctcgat cgtgagaagg ggctgttcac gtccgggatt catgtgctcg atgagctgtc
     2941 agtccgggca ctcagtcgtg acatcatgaa acagaaccgg gtgaccgtac atccggagaa
     3001 aagtgtcccc cggacggccg gttacagcga tgccgtcagt gttctggcac aggatcgccc
     3061 gtcgctggcc attgtgtccg ggcagggcgg tgcagccggg cagcgtgagc gggtggctga
     3121 actggtcatg atggcccggg agcaggggcg ggaggtgcag attatcgctg ctgaccgtcg
     3181 ctcgcagatg aacctgaagc aggatgaacg gctgtccggt gagctgataa ccggacgtcg
     3241 tcagctgcag gagggcatgg tcttcacgcc gggcagtact gttatcgtcg accagggcga
     3301 aaaactctcc ctgaaagaga cgttaaccct gctggatggt gccgcacgtc ataacgtaca
     3361 ggtcctgata accgacagcg ggcagcgaac cggtacaggc agtgcgctga tggccatgaa
     3421 ggatgccggg gtgaacacgt atcgctggca gggcggagaa cagcgaccgg caaccatcat
     3481 cagtgaaccg gaccggaatg tccgctatga ccggctggcc ggagattttg cggccagcgt
     3541 gaaagccgga gaagagagcg tggcacaggt cagcggggta cgggaacagg ccatactgac
     3601 acaggccatt cgcagtgagc tgaaaacaca gggcgtgctc ggacacccgg aggtgaccat
     3661 gaccgccctg tcaccggtct ggctggacag ccggagccgt tatctgcggg atatgtaccg
     3721 accggggatg gtgatggagc agtggaaccc ggagacacgc agtcatgacc gctatgttat
     3781 cgaccgggtg acggcgcaga gtcacagcct gaccctgcgg gatgcgcagg gcgaaacgca
     3841 ggtggtgcgt atttcctccc tggacagcag ctggtcgctg ttccggccgg aaaaaatgcc
     3901 ggtggcagac ggcgagcgac tgagggtgac agggaaaatt cccggactcc gcgtctccgg
     3961 cggtgaccgc ctgcaggtgg catccgtcag tgaagatgcg atgacggttg ttgtgccggg
     4021 gcgggctgaa ccggccaccc tgcctgtggc tgattcaccg ttcacggcac tgaagctgga
     4081 gaacggctgg gtggaaacgc ccgggcattc cgtcagtgac agtgcgacgg tgtttgcctc
     4141 cgtcacacag atggcaatgg acaacgccac cctgaacggt ctggcccgca gcggtcgtga
     4201 tgtccggctg tattcctcac tggatgaaac ccgtactgcg gaaaaacttg cccgccatcc
     4261 ctcctttacg gtggtttctg agcagataaa ggcgcgggcc ggtgagacat cgctggaaac
     4321 cgctatcagt ctgcagaaaa ccgggctgca cacgccggca cagcaggcta ttcatctggc
     4381 ccttcctgtg ctggaaagta aaaacctggc cttcagcatg gtggacctgc tgacagaggc
     4441 gaagtcgttt gctgcagaag gaaccagttt tactgaactg ggaggggaaa tcaatgcgca
     4501 gataaaacgc ggtgatttac tgtatgtgga tgtggcaaaa ggctatggca caggcctgct
     4561 ggtttcccgt gcgtcgtatg aggcagaaaa gagcattctt cgccatattc tcgaaggtaa
     4621 ggaggcggtc acgccgctga tggagagagt acctggcgaa ctcatggaga aactgacatc
     4681 aggacagcgt gccgccaccc gcatgatact ggaaacgtcc gaccgtttca cggtggtgca
     4741 ggggtatgcc ggtgtgggga agaccacaca gttccgggcg gtgatgtcat ccgtgaacat
     4801 gctgccggag agtgaacgtc cccgtatcgt tgggctgggg cccacgcacc gtgcggtcgg
     4861 tgagatgcgc agcgccggcg tggatgcaca gacactggcg tcctttctgc atgacacgca
     4921 gctgcagcag cgcagcggag aaacgccgga tttcagcaac acgctgttcc tgctcgatga
     4981 gagctcaatg gtgggcaata ccgacatggc acgggcatac gccctgattg cggccggtgg
     5041 cggtcgtgcc gtggccagtg gtgacacgga ccagctgcag gccatcgcgc ccggtcagcc
     5101 tttccgtctg cagcagacgc gcagtgctgc cgatgtggcc atcatgaaag agattgtgcg
     5161 tcagacgccg gaactgcggg aggcggtata cagcctgatt aaccgggatg tggaaagggc
     5221 actgtccggg cttgagagtg tgaaaccgtc tcaggtgcca cgtcaggagg gcgcatgggt
     5281 accggagcac tccgtgacgg agttcagtca cagccaggaa gcgaaactgg cagaagcgca
     5341 gcagaaggcg atgctgaaag gcgaggcttt cccggatatc cccatgacac tgtatgaagc
     5401 cattgtccgc gactataccg gcaggacacc ggaagcacgg gagcagacgc tgattgtcac
     5461 acacctgaat gaggaccggc gcgtactgaa cagcatgatt catgatgcac gggaaaaggc
     5521 cggtgagctg ggcaaagagc aggtcatggt gcctgtcctg aacacagcga atatacgtga
     5581 cggagaactg cgtcgtctct ccacctggga gaataacccg gatgcccttg ccctggtgga
     5641 tagtgtgtat caccggattg ccggtatcag caaggatgac gggctgataa ccctggagga
     5701 tgcggaaggt aacacgcggc tgatttcgcc ccgggaggcg gtggctgaag gcgtcacact
     5761 gtacaccccg gacaccatcc gggtgggaac cggtgaccgg atgcgcttca cgaagagtga
     5821 ccgggagcgc ggttatgtgg ccaacagcgt ctggacggtg atagcggttt ccggtgacag
     5881 tgtcacgctg tcggacggac agcagacccg ggtgattcac cccggtcagg agcgggcaga
     5941 gcaacatatt gacctggcct atgccatcac cgcccacggt gcgcaggggg caagtgaaac
     6001 ctttgccatt gcgcttgaag gcacggaagg taaccggaaa ctgatggcag gctttgagtc
     6061 agcctacgtg gccctgtcgc gtatgaagca gcatgtgcag gtgtacaccg ataaccgtca
     6121 gggctggacg gatgccatca acaatgccgt acagaaagga accgcccacg atgtgctgga
     6181 gccgaaaccg gaccgggagg tcatgaatgc agagcggctg ttcagtacgg cgcgggagct
     6241 gcgggacgtg gcggcaggcc gtgccgttct tcgtcaggcg gggctggccg ggggagacag
     6301 tcctgcacgg tttattgcac cggggcgcaa atatccgcag ccgtatgtgg cactgccggc
     6361 gtttgaccgt aacggcaagt cagccggtat ctggctgaac ccgctgacca cggatgacgg
     6421 aaacgggctg cggggattca gtggtgaagg ccgggtgaaa ggcagcgggg atgcgcagtt
     6481 cgtggccctg cagggcagcc gtaacggcga gagcctgctg gcggataaca tgcaggatgg
     6541 cgtccggatt gcccgtgata accccgacag cggtgtggtg gtgagaatcg ccggtgaagg
     6601 tcgtccgtgg aatcccggtg ccatcaccgg tggtcgcgtg tggggggata tcccggacag
     6661 cagcgtccag ccgggagccg gaaatggcga accggtcacg gcagaggtgc tggcacagcg
     6721 gcaggctgaa gaggccatcc gccgtgaaac ggaacgccgc gcagatgaaa ttgtcagaaa
     6781 aatggcagag aacaaacctg acctgccgga tggcagaaca gagcaggctg tcagggagat
     6841 tgccgggcag gagcgtgacc gggctgccat aactgaacgg gaagccgcgc tgccggagag
     6901 tgtgctgcgt gaatcacaac gggagcggga agcggtccgg gaggttgccc gggaaaatct
     6961 gctgcaggag cgtctgcagc agatggagcg ggatatggtt cgtgacctgc agaaagagaa
     7021 aaccctgggc ggagactgat acaggaagac aaacactgat gacaacggat aacacgaaca
     7081 cgacacgtaa cgattcactg gctgcccgga ccgatacctg gttgcagtcg tttctggtct
     7141 ggtcacccgg acagcgggat atcatcaaaa cggtggcact ggtgctgatg gtactggacc
     7201 acatcaatct gat
//
LOCUS       DAJOSY010000159         7181 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJOSY010000159 DAJOSY010000000
VERSION     DAJOSY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.159"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            600..1154
                     /locus_tag="OHN27_004026"
     CDS             600..1154
                     /locus_tag="OHN27_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288372.1"
                     /translation="MQDSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDIFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINSSSVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIV
                     VGNISLTISEPDFEFDVYAQAEEF"
     gene            1156..1437
                     /locus_tag="OHN27_004027"
     CDS             1156..1437
                     /locus_tag="OHN27_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288373.1"
                     /translation="MKNKHTLTRITKTAFDELNRIKNITGLPHSAVMKLAINRDINEQ
                     DLLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLAMEV"
     gene            1437..1823
                     /locus_tag="OHN27_004028"
     CDS             1437..1823
                     /locus_tag="OHN27_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288374.1"
                     /translation="MYDFYKHRDHLVRFDGKVWKDVTQTNKPKFTHQQLASRFDIWAE
                     AQKDNGDFEFIRGFDYAYESDLKDAIELMESFNSIKNSDKHKANGNRYDKDLIIDAIT
                     KGQSQSYISEKYGLSRSQYYKIKASV"
     gene            1962..2285
                     /locus_tag="OHN27_004029"
     CDS             1962..2285
                     /locus_tag="OHN27_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288375.1"
                     /translation="MNNFPLQIFIDTDTAMMIQAFTDVGVSIDFDKLLELMADNAESI
                     SDFIHSVEFNEPRMMLPITDSNMKRLVIEQTNKYSVSPERYLKAAIAILYADNILVTD
                     SKVVH"
     gene            2803..3513
                     /locus_tag="OHN27_004030"
     CDS             2803..3513
                     /locus_tag="OHN27_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2288376.1"
                     /translation="MTNDLFINGRKIDSFGDWSPQTEHPAITIQRKEHDERIALEQEL
                     KMSPKVISFVSPEPQEMPDVCKTEALLELEREYYPLLKAQRIKLDIAYDNVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAVEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAL"
     gene            3536..3745
                     /locus_tag="OHN27_004031"
     CDS             3536..3745
                     /locus_tag="OHN27_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288377.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKHHVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            3842..5890
                     /locus_tag="OHN27_004032"
     CDS             3842..5890
                     /locus_tag="OHN27_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCQ2288378.1"
                     /translation="MSKFTVDSFIVELGFNENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLKSMEGKINKTFDRLEARAKNTKAFRFTSQVNDAINPPKKSKP
                     RRVTSDRAITAAYSANMTKLKGLNPIVEKYIKSQFYALSSKAGQMDNTKFNEALAKLN
                     SSVREAIAKARGHTSTSINSDKAANSLDSLSSAAVKTAGAFISMQAVLASYQKIMEIG
                     LQRASAERSIDFVFDKDAGQVKEFTKSLAQTTGLDIAELQSQFAGFGASAKESMGIQG
                     SEELFRNMIGYSRLMGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVF
                     AKATGKSEQELFDAMKKGALKSADTLQKVTQELNRQITAKGGWKAISESTQAQLGNLK
                     NSWNTTLDSIFRGSENGLQDFTRSLTTLLNALGGSGKSLGESLGSLMTSMSSGVDSLT
                     DISYRVRAFFDEVTLAYRGLTDEQKKVVDALGSGLINSLKVLAGVIAVNKVVGVTRSI
                     YGLMTAITRLGQVADTTADNVNRKNGKPSRLNPMNMLLAGSAIYAGYERLASTPERME
                     AQAKLPELAKESGLKPVFTMDDVKGWFNSAAMNLANFQHPELITMRQDAALTNTDIQS
                     LRDEISALSKRIQEPVKVSLGGEVAIKPDETSFMTFSSNIYDQYAEATLLSSSFPEDD
                     "
     gene            5987..6751
                     /locus_tag="OHN27_004033"
     CDS             5987..6751
                     /locus_tag="OHN27_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288379.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDISAATG
                     KAATTNDGGATSKKNTGNTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSV
                     KNGESTFTASANEQASKVLKSGNALRKFSVNPNKRY"
     gene            complement(6766..>7181)
                     /locus_tag="OHN27_004034"
     CDS             complement(6766..>7181)
                     /locus_tag="OHN27_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2288380.1"
                     /translation="HREHISSNIEKLVQLSLEIEDLSAITSKLKQLKGELSQVDADIE
                     KETQRLKSIDVGCLSDCDLTTKAGRIEAQLIIKSAVKEVVLNTAKRRCKVTFHNGKVI
                     DLPITETPSEDVTEAIQSLSEVTERGLIDVDEVII"
BASE COUNT         2334 a         1309 c         1496 g         2042 t
ORIGIN      
        1 gtgaatgctt gatagataca gctttccggt tctgtcaagc tatttattaa tagatggcac
       61 aaaatgctaa agacacccct gtaccataga cttctatctg tcatctcgtg caggggtttt
      121 gaaggagttt aacgggggtc tgtgtggtcg ttatccttaa ggtcaagcgt ccatgtacga
      181 ccgaacagtt taaagacaac aagcactctg acgaggacgt agcgaagtag caggcctgca
      241 atcagcccaa tacctccacc agtccataca tcaggtgacc ccagctcagg agggagattt
      301 accattagtt ttccttgtaa gacgctctca atcgcattct gacacaaaaa gtacctgacc
      361 ttatgagttg acctgaagag gtcgtttaac tcgttacagg gcgattgaag aggtctgtgt
      421 gcaatgcaaa atatgcgacc aaatacatag ccacgcctca tattttactc tccccataaa
      481 cccattatac cacagatgtt acgaaaatgt taaaaatact tgacattagt ctttgaaaat
      541 gctaatattg tatatatagg ttaacagaat aataacatct tatgagggtc agaacatgaa
      601 tgcaggattc agaatttaga gcgatgcttc aggcatcaag agaacgcaac aagcacaact
      661 cctacgctta cacaaacaac cccaccagct acgaagtccc aacctttagc aaatccgaac
      721 gtaagaacat tgaagcggtc attcgtagca ttacgccacg agacagattt atgccagtac
      781 gcaaaacaac aaagaatact attaaaaatt atctggctaa ctttgacagt tacgagcagt
      841 taccaagcaa gcttgatgat atttttattg ggttctgtag aagtgaaggc cacccgaaat
      901 acaacaaaaa actattttat cttctaaaga atcttgatga aattaactca tccagcgtga
      961 caaatcatct tcagcgacaa gcaaccagac ttagctacga attaccaagc gataaatatt
     1021 gtgcattgct tgctgtaatg tgtgcaaagc taattggtat tgttgaacat catattgtcg
     1081 ttggtaatat cagccttacg attagtgagc cagactttga atttgacgta tacgcacaag
     1141 cagaggaatt ttaaaatgaa aaacaaacac actttaaccc gtattacaaa gacagccttt
     1201 gacgaactta accgaatcaa aaacattaca ggtctgccac actcagcagt gatgaagctt
     1261 gcaattaacc gtgatattaa cgagcaggat ttgcttaata gtcgtttaga ggcagcaaga
     1321 caccatatca ggattaagca agatgacctt gatagtctga aatccttaaa tgcttttgga
     1381 ctggatttag caaggttact aagtatcaaa attcacaaat tagcgatgga ggtttaatgt
     1441 atgactttta taaacatcgt gaccatttgg taagatttga cggtaaagtc tggaaagacg
     1501 taacacaaac caataaaccc aagtttaccc accagcaatt agcctcaaga tttgatatct
     1561 gggctgaagc acaaaaagat aatggtgatt tcgagtttat cagaggtttt gattatgctt
     1621 acgaaagcga ccttaaagac gccattgaat taatggagag cttcaactct attaaaaata
     1681 gtgacaagca caaagccaac ggtaacagat acgataaaga tttaatcatt gatgcaatca
     1741 ccaaagggca atcacaaagt tatatttctg agaaatacgg cttatcacgt agtcaatact
     1801 acaagattaa agcgtctgtg taagtctgtg agaatgagtg agactcaaag atgagttacc
     1861 ctattactaa ggtgtctcac tccctctgta acccgcatgg ttactggcct cagaatggct
     1921 attttggcag atttaagatg tgtttttata ggattttaaa aatgaataat ttcccactac
     1981 agatttttat tgataccgac accgccatga tgattcaggc tttcactgat gtaggtgtta
     2041 gcattgactt tgataagttg ttagagttaa tggctgataa tgcggaaagc atctctgact
     2101 ttattcactc ggtggagttt aacgagcctc gaatgatgtt accaattaca gacagtaata
     2161 tgaaaagatt ggttattgag caaacgaaca aatacagcgt tagccctgaa agatatctca
     2221 aggcagctat agcaattttg tacgctgaca atattttggt gacggattcg aaggtggtac
     2281 attaatggaa ttggtgatta tctgttttgt attggttggt atgattgcag gtgcgtctat
     2341 taaagtatag attgttatca atctgttaaa agctgttgac aaacaacctt gagcatgaca
     2401 taattgtata tataggaaga gcaatttttc ctaaaatcat attttcaata atccgatgga
     2461 tggatattct gagttggaaa ggtgcaatgc ttttcgtcat ggcctctctt atataacttg
     2521 agaggctatc tcaaaaagtc tttgttcatt cgtttgaatc ctttacagct ttgccctgac
     2581 tcatgattag ggctttcttt aagagttttt aaacagggct tttaaagaaa gtttttcctg
     2641 aaacttcgtt aacgttataa agcaaacctc caaagccctt cacagattat gtttagggct
     2701 tttcttaata gagcatcttt aataaatcgt taagttatct tctaagtgta ttggcgttga
     2761 aggtgctcta ttaagaaata accacactta gaggaaataa taatgacaaa tgatttgttt
     2821 attaacggtc gaaagattga tagcttcggt gactggtcgc cacagactga acatccagct
     2881 atcactattc agcgtaaaga acacgatgaa agaattgcac ttgagcaaga gttaaagatg
     2941 tcaccaaagg taatttcctt tgtgtctcct gagcctcaag agatgcctga tgtatgcaaa
     3001 actgaagcat tacttgagct tgagcgtgaa tattacccac tgctgaaagc acaaagaatt
     3061 aaacttgata ttgcttacga caatgttaag aacttcgaag aaacctcgaa accttcagag
     3121 tatgatattg cttacgagat taagtcaaac ccatttatct attatgaagg aaactttaat
     3181 gatggttttg gtacagcggt cgaagacgtt ccaaaagttc ttcaaagtat ccctgaaggg
     3241 ttccggttaa ttgatgtcag acaagcatta aggggttctg gtcaatttat tctgatgact
     3301 gataaaacag atgaagagat tcttcagtta gctgaagcta atttgactaa gcattattcc
     3361 ggtaaactgg ataagttaaa gcaaaagctt aaaaaagaac ttgatactat gaaagaactt
     3421 gttagtgaat atgagtcgca gaagaagatt gctttgatgg ctgatattga gcagttaacg
     3481 aagatttctc agaagtatag caaggcactt tgattatgaa ttaaagaggt gattaatgtt
     3541 tgaagaacgt ttagagacta ttcggtctgt ttgtgaaaac ttaaagttac aaactaagcc
     3601 agccttaaga attaaaaaca aacatcatgt tatcacctca cacaaaccaa aaacacgcaa
     3661 gattccaaag tggtgtatcg accgtattcc ttctgatgct caaatcatag gtgaaacgga
     3721 attacattat cttgtccgac attaaaaaag aattggttgg gtaaatacgg ggagagatta
     3781 accttaattg gttggtcctc tcctttttcg tatatgaaca cttaagaggt gaaaaatatg
     3841 aatgagcaaa ttcacagttg atagttttat cgttgagcta ggcttcaatg agaatgttat
     3901 taaaggtctg caaagagttg aaaaggcagc tttgcagtca gcacaaagaa ttgaacgtaa
     3961 tttgaataga gcgtttaagg tcgataccaa gcaacttgac agcaatctga caagttcttt
     4021 gaagtcaatg gaagggaaaa ttaataagac ctttgaccgt ctggaagcta gagcaaaaaa
     4081 tactaaagct ttccgtttca caagtcaggt taatgatgct attaatcctc caaaaaaatc
     4141 aaagccaaga agagttacta gtgaccgagc tattacagcg gcttattcag cgaatatgac
     4201 caagctgaaa ggtcttaacc caattgtcga gaaatacatt aagagccagt tttacgcatt
     4261 aagtagtaaa gctggtcaga tggataacac caagtttaat gaagcccttg ctaaactaaa
     4321 ttcaagtgta cgagaagcaa ttgctaaagc cagaggtcat acatccacca gcattaatag
     4381 tgacaaagca gctaatagtc ttgatagtct gtcttcagca gcagttaaaa cagcaggtgc
     4441 atttatctca atgcaggctg tattagccag ttatcagaag attatggaaa ttggccttca
     4501 acgtgcaagt gctgaacgtt caattgattt cgtatttgat aaagatgcag gtcaagtaaa
     4561 agagttcaca aagtcattag ctcaaacaac gggtttagat atcgctgaac tgcaatcaca
     4621 gtttgctggt ttcggtgctt cagctaaaga gtctatgggt attcaaggtt ctgaagaact
     4681 ttttagaaat atgattggct attctcgttt aatgggtcga agtgaagaag agattaagcg
     4741 agcactgaca gcattatcac agatggctgg taagggtcag attatggctg aagagcttaa
     4801 ggggcaacta gctgaagctg ttcctggtat ggttcaggta ttcgctaaag caaccggaaa
     4861 gagtgaacag gaattatttg atgccatgaa gaagggggct ttaaaatctg ccgatactct
     4921 tcagaaggtc actcaggaac tgaatagaca aatcaccgct aaaggtggct ggaaagccat
     4981 ttcagaaagt acacaggcac aactagggaa cttgaaaaac tcttggaata ccacgcttga
     5041 tagtatcttc agaggttctg aaaatggtct gcaagatttt acacgaagcc taacaacgct
     5101 tttaaatgct cttggtggaa gtggtaagag tcttggtgaa tctcttggta gtctcatgac
     5161 ttctatgtca agtggtgtcg atagtctgac tgatattagt tacagagtga gagcgttctt
     5221 tgatgaggtg acattagctt accgtggttt aaccgatgag caaaagaaag tagttgatgc
     5281 tttaggttct ggcttgatta atagtttaaa agtcttggct ggtgttattg ctgtaaataa
     5341 agttgttggt gtaaccagaa gtatttacgg gcttatgact gctatcacaa gattaggtca
     5401 ggtcgctgat acaacagcgg ataacgtgaa tagaaagaat ggaaaaccat caagactaaa
     5461 ccctatgaat atgctattag ctggtagtgc tatctatgca ggttatgaaa gattagcttc
     5521 tacacctgaa cgaatggaag cacaagctaa attaccagag ctggctaaag aatctggact
     5581 taagccagta tttactatgg acgatgtgaa aggttggttt aatagtgcag ctatgaatct
     5641 tgctaacttc cagcatccag aattaatcac catgagacaa gatgcggcat taacgaatac
     5701 ggatatccaa agcctgagag atgaaattag tgctttgagc aaacgtattc aggagcctgt
     5761 gaaagtttca cttggtggtg aagttgccat taaaccagat gaaaccagct ttatgacctt
     5821 tagcagtaat atctacgacc aatacgcaga agctacgcta ttaagttcat cattcccaga
     5881 agatgattga taatagtcga atgctacgtg atttcttctg aacagatacc aacatcaaaa
     5941 attagatttc acgtagctca cgcttaacag aataggaata acaacaatgt ttgacacaaa
     6001 gcaaattaga tactcaggta aagatggtat ctattttcac cttagagata atgtagaatc
     6061 ttttcttgcc ttaacagcta ctgaacagat ggactatgac tcaccgatga tggtgacaac
     6121 tcaggctatg caatcggggc aaactataac tgataatgtt cagagagcac ctaagaccat
     6181 ctctattagc ggtgttgtag tggttgattc gacgggttca gttctattaa ccagacaggg
     6241 gcaacttgta gaaaccttta tttctacggt tgaacaatgg cgagaccaga agcaaatcat
     6301 cactattgtt tgtagagatg gaatagctat cactgatagt atcattactc agtttaaagc
     6361 gagtaaagag cataccattg ctaatggtct tcgtattcag atgaccttcc aaggtgtaga
     6421 gtttcgttca atcgtaggac aaactgatat ctcggctgcg actggaaagg ccgctaccac
     6481 gaatgatggt ggtgctactt ctaagaagaa tacagggaac actacaacga gccttggaag
     6541 ccctatgctg acctgtaaag agctttttag ctattcggca agtgagttat cagatgaagc
     6601 gctaaaagcc cgtgtaacgt gctctaagag cgttagcgtt aaaaatggtg agagcacctt
     6661 cacagcctca gctaatgagc aagcttcaaa ggtgcttaag tcgggtaatg ctttacggaa
     6721 gtttagtgtg aatcctaaca agcgctactg acgatagaat ctcaactata tgataacctc
     6781 atcaacatct atcaaccccc tctcagtgac ttcagaaagc gactgtatag cctctgtaac
     6841 gtcctctgag ggggtttctg ttattggtag gtcaattacc ttaccgttgt gaaaagttac
     6901 tttacatcga cgtttagcag tattcaaaac gacctcttta acagcacttt tgattatcag
     6961 ttgtgcttca atccttcccg cctttgtagt caagtcacaa tcagacaaac accccacatc
     7021 aatagattta aggcgttgtg tctccttctc gatatccgca tcaacctgtg atagctcacc
     7081 ttttaactgt tttagtttgc tggtaatagc ggacaagtct tcaatttcaa gtgatagctg
     7141 taccagcttt tcaatattgc ttgagatgtg ttcacggtgt a
//
LOCUS       DAJOSY010000160         7142 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJOSY010000160 DAJOSY010000000
VERSION     DAJOSY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.160"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(156..1019)
                     /locus_tag="OHN27_004035"
     CDS             complement(156..1019)
                     /locus_tag="OHN27_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCQ2288381.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(991..2541)
                     /locus_tag="OHN27_004036"
     CDS             complement(991..2541)
                     /locus_tag="OHN27_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCQ2288382.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            2799..3578
                     /gene="deoC"
                     /locus_tag="OHN27_004037"
     CDS             2799..3578
                     /gene="deoC"
                     /locus_tag="OHN27_004037"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCQ2288383.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            3705..5027
                     /gene="deoA"
                     /locus_tag="OHN27_004038"
     CDS             3705..5027
                     /gene="deoA"
                     /locus_tag="OHN27_004038"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCQ2288384.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            5079..6302
                     /gene="deoB"
                     /locus_tag="OHN27_004039"
     CDS             5079..6302
                     /gene="deoB"
                     /locus_tag="OHN27_004039"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCQ2288385.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            6359..7078
                     /gene="deoD"
                     /locus_tag="OHN27_004040"
     CDS             6359..7078
                     /gene="deoD"
                     /locus_tag="OHN27_004040"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCQ2288386.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1661 a         1891 c         1950 g         1640 t
ORIGIN      
        1 cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg ccggataagg cgttcacgcc
       61 gcatccggca gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta tcatgcctac
      121 aagtctgtgc cgaaccgtag gccgtatccg gcatgtcaca aatagagcgc cggaaatatc
      181 aaccggctca ccccgcgcac ctttaacgca tcagccaacg gctcaacgtc ttccggcgtg
      241 gcgctcgccc agctttgcgc ctcgccatac acgccgtggg catgaaacgc gttcaggcgt
      301 accggaacat cgccaagtcc cttgataaac accgccagtt cttcgatgtg ttgcaaataa
      361 tccacctggc cagggattac cagcaaacgc agttccgcca gcttaccgcg ctctgccagc
      421 aaacagatgc tgcgcttaat ctgctgatta tcgcgtccgg ttaattgttg atgacattcg
      481 ctcccccacg ctttgagatc gagcattgcg ccgtcacaca ccgggagtaa tttttcccag
      541 ccggtttcgc tcaacatacc gttactgtcc acgaggcagg tgaggtggcg cagttgcgga
      601 tcgtttttga tggctgtaaa cagcgccacc acaaacggca gctgggtcgt ggcttcgccg
      661 ccgcttaccg ttatcccttc gataaacagc accgctttgc ggatatggct aagcacctca
      721 tccacgctca tggattgcgc cattggcgtg gcatgttgcg gacacatctt caggcaggta
      781 tcacactgct cgcagaccac agcgttccac agcactttgc cgtcaacaat ctgcaacgcc
      841 tgatgcggac actgtggcac gcactctccg caatcattgc agcgtcccat cgtccacgga
      901 ttgtgacagt ttttgcagcg cagattgcag ccctgcaaaa acagagccag acgactgcct
      961 ggcccgtcaa cgcaggagaa ggggataatc ttactgacta aagcgcatct gctgttcatg
     1021 gcttatcacg cgcggctggc gttccagaat acgagtgttg cgtgcggctt cttcgcccag
     1081 ccaggtggtg ttggtgcgtg aaccttcggc gcgatatttt tctaaatccg acaaacgcac
     1141 catataaccg gtaacgcgaa ccagatcgtt accgctgaca ttggcggtaa attcacgcat
     1201 tccggcttta aaggcaccga ggcaaagctg taccagtgcc tgcgggttac gtttgatggt
     1261 ttcgtcgagg gtcagaatgt cgctgatgcc ggaataataa taagcatgat gcggggcgac
     1321 agtttgcaga tgggtgatcg gatctggctc atcgccatac ggcaaacgcg cgcccggcgt
     1381 ggtgccgata tcggaactga tccccgactg tgcgtgtaac atggcgcgtt tttgccagcc
     1441 atatttcacg ggggtattgg cgacaaactc cgccagttgc gcgctgatgc gataacccac
     1501 ttcatttgcg gcggcttctt taccgtagcg cgcggcaatc ccttctttct cacacagcaa
     1561 gttaaccgct tccgccagcc catacatgcc aaacattggc acaaaacgtt cagggttaat
     1621 cagcccttct ttcaccagga agctattctc aaagaagtgt gattgttgat agaggaattc
     1681 acaccgcgca tcgatgatgg cgatctgctg ctggcagtag tgcggtagag tgcgcgtaaa
     1741 gaagtcatcc agcgattcgc tgcgctcggc aatggctttc aggttaaggc gtaccagcgt
     1801 gctgccacca cccgccagcg gcagtgagtt gtaacagctc acaatcccgt agcccccttt
     1861 tgtgaaaatt ttatcatgca ccggaccgtt ggcgatgtgc ggtttgctac attcacagat
     1921 gttcttcgcc acttccagca gcaggtcatc aggggtgatt tctggatcgt agataaaggt
     1981 caggttcggt gaaacctgct tcaactctgc atctgcacgt aatatcgcac gggtgatggg
     2041 cgaatcagac gggccgatat tggcgtgcat aaaggcgtct ggcagggttc tgtcgaggta
     2101 acgccagaaa cgttttattc gaatatcgat ctcgtcttgt gttagaattc taacatacgg
     2161 ttgcaacaac gcatccagtt gccccaggta gaccggcatc gatgtgaccg acggtacgtg
     2221 gtggtaaaga atggtcagca gagagagtgc gtcatcaaga tctttcgcgc cttccagctc
     2281 cagccattcg gaaccgttcg ccagaaaacg ggcgtaatcg ggtaagacat agcgcggttt
     2341 gtacggcgca tgaccttcaa acatatcgca gattacaccc tcatccagcg cgcggcgggc
     2401 ctcggcaggc agctgcggat aaggcagatt gttttctgct tccagtgcca gaaaatggcg
     2461 cttctgctcc gggctaagca ctgggctggt gacaatttgc tggcaacgtt gttgcagtgc
     2521 attttcatga gaagtgggca tcttcttttc cttttatgcc gaaggtgatg cgccattgta
     2581 agaagtttcg tgatgttcac tttgatcctg atgcgtttgc caccactgac gcattcattt
     2641 gaaagtgaat tatttgaacc agatcgcatt acagtgatgc aaacttgtaa gtagatttcc
     2701 ttaattgtga tgtatatcga agtgtgttgc ggagtagatg ttagaatact aacaaactcg
     2761 caaggtgaat tttattggcg acaagccagg agaatgaaat gactgatctg aaagcaagca
     2821 gcctgcgtgc actgaaatta atggacctga ccaccctgaa tgacgacgac accgacgaga
     2881 aagtaattgc tctgtgtcat caggccaaaa ccccggtcgg caataccgcc gctatctgta
     2941 tctatcctcg ctttatcccg attgctcgca aaacactgaa agagcagggc accccggaaa
     3001 tccgtatcgc tacggtaacc aacttcccac atggtaacga cgacatcgaa atcgcgctgg
     3061 cagaaacccg tgcggcaatc gcctacggtg ccgatgaagt tgacgtggtg ttcccgtacc
     3121 gtgcgctgat ggcgggtaac gagcaggttg gttttgacct ggtgaaagcc tgtaaagagg
     3181 cttgcgctgc ggctaacgta ctgctgaaag tgatcatcga aaccggcgaa ctgaaagacg
     3241 aagcgctgat ccgcaaagcg tctgaaatct ccatcaaagc gggtgccgac ttcatcaaaa
     3301 cctctaccgg taaagtggca gtgaacgcga cgccggaaag cgcgcgcatc atgatggaag
     3361 tgatccgtga tatgggcgta gagaaaaccg ttggtttcaa accggcgggc ggcgtgcgta
     3421 ctgcggaaga tgcgcagaaa tatctcgcca tcgcagatga actgttcggt gctgactggg
     3481 ctgatgcacg tcactaccgc tttggtgctt ccagcctgct ggcaagcctg ctgaaagcgc
     3541 tgggtcacgg cgacggtaag agcgccagca gctactaagt aagatgcttt acgcctgatg
     3601 cgctgcgctt atcaggccta cgagacgtat ctacccgtag gccggataag gcgtagacgc
     3661 atccggcaaa agccgcctca tactcttttc cacgggaggt taccttgttt ctcgcacaag
     3721 aaattattcg taaaaaacgt gatggtcatg cgctgagcga tgaagaaatt cgtttcttta
     3781 tcaacggtat tcgcgacaac actatctccg aagggcagat tgccgccctc gcgatgacca
     3841 ttttcttcca cgatatgaca atgcctgagc gtgtctcgct gaccatggcg atgcgagatt
     3901 caggaaccgt tctcgactgg aaaagcctgc atctgaatgg cccgattgtt gataaacatt
     3961 ccactggtgg cgtcggcgac gtcacttcgc tgatgttggg gccgatggtc gctgcctgcg
     4021 gcggctatat tccgatgatc tccggtcgcg gcctcggtca tactggcggt acgctcgaca
     4081 aactggaatc catccctggc ttcgacattt tcccggatga caaccgtttc cgcgaaatta
     4141 ttaaagacgt cggcgtggcg attatcggtc agaccagctc actggctccg gcggataaac
     4201 gtttctacgc gacccgtgat attaccgcaa ccgtggactc catcccgctg atcactgcct
     4261 cgatcctggc gaagaaactg gcggaaggtc tggatgcgct ggtgatggac gtgaaagtgg
     4321 gtagcggtgc atttatgccg acctacgaac tctctgaagc ccttgccgaa gcgattgttg
     4381 gcgtagctaa cggcgctggc gtgcgcacca ccgcgctgct caccgatatg aatcaggtgc
     4441 tggcctccag cgcaggtaac gcggttgaag ttcgtgaagc ggtgcagttc ctgacaggtg
     4501 agtatcgtaa cccgcgtctg tttgatgtca cgatggcgct gtgcgtggag atgctgatct
     4561 ccggcaaact ggcgaaagat gacgccgaag cgcgcgcgaa attgcaggcg gtgctggaca
     4621 acggtaaagc ggcagaagtc tttggtcgta tggtagcggc acaaaaaggc ccaaccgact
     4681 tcgttgagaa ctacgcgaag tatctgccga cagcgatgct gacgaaagca gtctatgctg
     4741 ataccgaagg ttttgtcagt gaaatggata cccgcgcgct ggggatggca gtggttgcaa
     4801 tgggcggcgg tcgccgtcag gcatctgaca ccatcgatta cagcgtcggc tttactgata
     4861 tggcgcgtct gggcgaccag gtagacggtc agcgtccgct ggcggttatc cacgcgaaag
     4921 acgaaaacag ctggcaggaa gcggcgaaag cggtgaaagc ggcaattaaa cttgccgata
     4981 aagcaccgga aagcacacca actgtctatc gtcgtatcag tgaataacgg tatactgatc
     5041 tgatcattta aatttgaagc actgagtacg gagaacatat gaaacgtgca tttattatgg
     5101 tgctggactc attcggcatc ggcgctacag aagatgcaga acgctttggt gacgtcgggg
     5161 ctgacaccct gggtcatatc gcagaagcct gtgccaaagg cgaagctgat aacggtcgta
     5221 aaggcccgct caatctgcca aatctgaccc gtctggggct ggcgaaagca cacgaaggtt
     5281 ctaccggttt cattccggcg ggaatggacg gcaacgctga agttatcggc gcgtacgcat
     5341 gggcgcacga aatgtcatcc ggtaaagata ccccgtctgg tcactgggaa atcgccggtg
     5401 tcccggttct gtttgagtgg ggatatttct ccgatcacga aaacagcttc ccgcaagagc
     5461 tgctggataa actggttgaa cgcgctaatc tgccgggtta cctcggtaac tgccactctt
     5521 ccggtacggt cattctggat cagctgggcg aagagcacat gaaaaccggc aagccgattt
     5581 tctatacctc cgctgactct gtgttccaga ttgcctgcca cgaagaaacc tttggcctgg
     5641 ataaactcta cgaactgtgc gaaatcgccc gtgaagagct gaccaacggc ggctacaaca
     5701 tcggtcgtgt tatcgctcgt ccgtttatcg gtgacaaagc cggtaacttc cagcgtaccg
     5761 gtaaccgtca cgacctggct gttgagccgc cagcaccgac cgtgctgcag aaactggttg
     5821 atgaaaaaca cggtcaggtg gtttctgtcg gtaaaattgc agacatctac gccaactgcg
     5881 gtatcaccaa gaaagtgaaa gcaactggcc tggacgcgct gtttgacgcc accatcaaag
     5941 agatgaaaga agcgggtgat aacaccatcg tcttcaccaa cttcgttgac ttcgactctt
     6001 cctggggcca ccgtcgcgac gtcgccggtt atgctgcggg tctggagctg ttcgaccgcc
     6061 gtctgccgga gctgatgtct ttgctgcgcg atgacgacat cctgatcctc actgctgacc
     6121 acggttgtga tccgacctgg accggtactg accacacgcg tgaacacatt ccggtactgg
     6181 tttacggccc gaaagtaaaa ccgggttcac tgggtcaccg tgaaaccttc gcggatatcg
     6241 gccagactct ggcaaaatat tttggtactt ctgatatgga atatggcaaa gccatgttct
     6301 gatggatttg ggcggagcgt tgactccgcc tttgttatgt cacaaaaagg ataaaacaat
     6361 ggctacccca cacattaatg cagaaatggg cgatttcgct gacgtagttt tgatgccagg
     6421 cgacccgctg cgtgcgaagt atattgctga aactttcctt gaagatgccc gtgaagtgaa
     6481 caacgttcgc ggtatgctgg gcttcaccgg tacttacaaa ggccgcaaaa tttccgtaat
     6541 gggtcacggt atgggtatcc cgtcctgctc catctacacc aaagaactga tcaccgattt
     6601 cggcgtgaag aaaattatcc gcgtgggttc ctgtggcgca gttctgccgc acgtaaaact
     6661 acgcgacgtc gttatcggta tgggtgcctg caccgattcc aaagttaacc gcatccgttt
     6721 taaagaccat gactttgccg ctatcgctga ctttgacatg gtgcgtaacg cggtagacgc
     6781 ggctaaagca ctgggcgttg atgctcgcgt gggtaacctg ttctccgctg acctgttcta
     6841 ctctccggac ggcgaaatgt tcgacgtgat ggaaaaatac ggcatcctcg gcgtggaaat
     6901 ggaagcggct ggtatctacg gcgtcgctgc agaatttggc gcgaaagccc tgaccatctg
     6961 taccgtgtct gaccacatcc gcactcacga gcagaccact gccgctgagc gtcagaccac
     7021 cttcaacgac atgatcaaaa tcgcactgga atccgttctg ctgggcgata aagagtaatt
     7081 gtgtttcgct gaaaggcgat tgtcgtgtga agccggagtg ggagactgct ccggcttttt
     7141 ag
//
LOCUS       DAJOSY010000161         7070 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJOSY010000161 DAJOSY010000000
VERSION     DAJOSY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.161"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..126
                     /locus_tag="OHN27_004041"
     CDS             <1..126
                     /locus_tag="OHN27_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCQ2288387.1"
                     /translation="LGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRLMAD"
     gene            376..618
                     /locus_tag="OHN27_004042"
     CDS             376..618
                     /locus_tag="OHN27_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288388.1"
                     /translation="MNISIKNNDLKTVARFDGKTLRDAFSRKIASFDGKVLRDASLKK
                     VATFDGQNLRDASSKIILSADAEVGIVVMAFVAELI"
     gene            1192..2331
                     /locus_tag="OHN27_004043"
     CDS             1192..2331
                     /locus_tag="OHN27_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HCQ2288389.1"
                     /translation="MNKPRFNGTPVASLDSLSAMLGIERKRLDWIVKSVSMSYKQFKV
                     ETGKNKKERQIFEPKRSLKGIQKKINKEIFEKIDYPHYLHGALSGRDYISNAAVHTRK
                     RTVICLDITNFYPSISKKDVCSIFKNLMRFSPDVSLCLTELVTLNNKVPQGGCCSSYI
                     ANLLFFNSEYNIYNRLKSMGLSYSRLLDDITISSDKDLSSEEKTKVIKLVHGMVNQYR
                     LSINESKTTIEHSKDSSSKLSVTGLWVKHGVPKLTKENRRYIRYLVYICKKQGAYERH
                     TKEYHDLWNRCSGKVAQMSRLGHVQAVELRAILSEIMPVYDDYKISKLKLMAKHYLNK
                     FTPPLTDDQIRKIDRMLYDFDIVGRTNKNLAKLYRRKLVALLPDR"
     gene            2336..2539
                     /locus_tag="OHN27_004044"
     CDS             2336..2539
                     /locus_tag="OHN27_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Se72 family effector protein"
                     /protein_id="HCQ2288390.1"
                     /translation="MENGFVNFYDHVKGYGFIRRERGRDVFFRYDDFLFLGHDVDICK
                     GILVRFKLEKTDKGFKAVAIQKV"
     gene            complement(3347..6235)
                     /locus_tag="OHN27_004045"
     CDS             complement(3347..6235)
                     /locus_tag="OHN27_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_073984355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HCQ2288391.1"
                     /translation="MGKSRGDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQ
                     LGIRDITVLAEYGQRENTRREHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERP
                     GLLFDLATGWLMQHRIILPGATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEML
                     LGPTDCSRLSLLESLKKGPVTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNL
                     ARYAGMTSVFNIARMSPQKRMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIG
                     QKKRLRSLKDLDKSALALASACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREI
                     ARPSDDNFHEEMVEQYGRVRRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQF
                     FDDAPTEIISRSWKRLVINKEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPR
                     ARLLQGADWQANRIKVYRSLGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDV
                     SGPKPRLTISPLASLDEPDSLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHAS
                     EASARVDDLPVSISAVLMAEACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSA
                     NARLVDFQATLPLAQIWGGGEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFV
                     SDQYSGFHGIVIPGTLRDSIFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGY
                     QFSPRLADAGASVFWRMDHDADYGVLNDIARGQSDPRKIVLQRDEMIRTAGSLKLGKV
                     QVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRH
                     AVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETL
                     NDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLRPLKEASEAENVA"
     gene            6335..7039
                     /locus_tag="OHN27_004046"
     CDS             6335..7039
                     /locus_tag="OHN27_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HCQ2288392.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT         1867 a         1573 c         1745 g         1885 t
ORIGIN      
        1 ctgggggcgt tacagaaaaa actctttatc gacggcaagc gtgtcgggga cagggagtat
       61 gcggaggtgc tgatcgctat tacggagtat caccgtgccc tgttatccag gcttatggca
      121 gattagcttc caggggagaa aactgtcgaa aacagacggt atgaacactg taagctccca
      181 aagtgatcac cactcgcttt catgcatagc tatgcagcga gctgaaaacg atcctgacgc
      241 atttccccgg ttttcttagg gtaaaacatc tctttttgca gtgtctgtgt cagaatcgcg
      301 ctgagcgcga ttctgtggtg cgtacaatta agggattatg gtaaatacaa ttggttaaat
      361 tactattggt gagaaatgaa tatttcaata aaaaacaatg acttaaaaac agttgctcgg
      421 tttgacggta aaaccttaag agatgccttt tcgagaaaaa tagcgtcttt tgatggcaag
      481 gttttaaggg atgcctcttt aaaaaaagta gcaacctttg acggtcagaa tttaagagat
      541 gctagttcaa aaattatcct gagtgccgat gctgaggttg ggattgttgt tatggccttt
      601 gtagctgagt tgatttgaat ctcatgctgc atacctttta gcctatcgct ggcaggataa
      661 ggcgctttct tgttggtgac agaaagataa aaaactttat aataatcagc gaagcgggcg
      721 aaccacgcct ggctctcgct ctttaacaaa tggttacgtg tatgttgcgc aaggaaaccg
      781 atgccttgtt tttaaggcga tctcgcatga gatcatgacc ggcctggccg ctaattaatg
      841 gtgagaacgc ccctgagcgt gtatcagcac taaaagaata gcgacgattg tctggcaatg
      901 tgagttaaaa gtcccaaaga gccaatagag tttttccttg ctttcctact ggtctggcaa
      961 aatttcataa ccaataggag ttgcatcatg tttattatca acatcaacat taacgtaacc
     1021 aagagctctt catcttgttc tggagacggt cagatgaaga aaaaaagaga ggttggctct
     1081 ctggaggcac tgaaaaaaac acagtttttg ttttctatgt gcttgattat gtttaaagta
     1141 atagattacg tatcgaaact ttttaactaa tcgactttta actcaaacat catgaataaa
     1201 cccaggttta atggaactcc tgttgcatct ttggattctt tatctgcgat gttaggcata
     1261 gaaagaaaaa gattggactg gatagtcaag tctgtatcga tgtcgtacaa gcagtttaag
     1321 gttgaaacag ggaagaataa aaaagaaagg caaatttttg aacccaagag atctttgaaa
     1381 ggaattcaaa aaaaaataaa taaagagatt tttgagaaaa ttgattaccc tcattatttg
     1441 catggggcgt taagtggtcg cgattatata tctaatgccg ctgttcatac taggaaaaga
     1501 acagttatat gtcttgatat caccaatttt tatcccagca ttagtaaaaa agatgtttgt
     1561 tctattttta aaaatttaat gcgtttttct cctgatgttt ctttatgctt aacggaactt
     1621 gtgacgttga ataataaggt ccctcagggt gggtgctgtt ctagttatat tgcaaatctt
     1681 ttatttttta atagtgagta taatatctat aatagattga agtctatggg gctttcatat
     1741 tcaaggcttt tagatgatat tactatatcc tctgataagg atctttcatc agaggaaaaa
     1801 acaaaagtaa taaaactcgt tcacggaatg gtgaatcagt acagactttc aataaatgaa
     1861 agtaagacaa cgatagagca ctcaaaagat tcgtcttcta aacttagtgt cactgggttg
     1921 tgggtaaagc atggtgttcc taagttaaca aaagaaaata gacgttatat caggtactta
     1981 gtttatatat gtaaaaaaca aggtgcttat gaacgtcata cgaaagagta ccatgattta
     2041 tggaatagat gctctggcaa ggttgctcaa atgtcgaggc ttgggcatgt ccaggcggtt
     2101 gaattgaggg cgattctttc tgagataatg ccagtttatg atgattacaa gatatctaaa
     2161 ttaaagttga tggctaagca ctatctaaat aagtttacgc ctcctttaac tgatgatcag
     2221 attaggaaaa tagataggat gctctatgat tttgatatag tagggcggac taataagaat
     2281 cttgcaaaac tatatagacg taagctcgtg gcattacttc cagataggtg attatatgga
     2341 aaatggattt gttaattttt atgaccatgt taaagggtat ggctttataa ggcgtgagcg
     2401 tggacgtgat gttttctttc ggtatgatga ttttttgttt ttagggcatg atgtcgatat
     2461 atgtaaaggt attttagtta ggtttaaatt agaaaaaaca gataaagggt ttaaagctgt
     2521 agcgattcaa aaggtataac atcgtggatg tgtcagcttg tttttatgat atgagaactt
     2581 gagatccttt ctatctcgtc gtaatctctt gctgtgtaaa cgaaaaaacc gccttgcagg
     2641 gcggtttttc gaaggttctc agagctacca actctttgag ccgaggtaac tggcttggag
     2701 gagcgcagtc accaaaactt gttctttcag tttagcctta acaggcgcat gacttcaagg
     2761 ctaactcctc taattcggtt accagtggct gctgccagtg gcgataagtc gtgtcttctc
     2821 ggtttggatt caagtcgata gttaccggat aaggcgcagc ggtcggactg aacggggggt
     2881 tcgtgcatac agtccagctt ggagcgaacg acctacaccg ggccgagata ccaacagcgt
     2941 gagctatgag aaagcgccac gtttcccgaa gggagaaagg cggacaggta tccggtaagc
     3001 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3061 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc ggtttttgtg atgctcgtca
     3121 ggggggcgga gcctatggaa aaacgccatc ggcgcggcct ttgtccctgg cttcggcttt
     3181 ttgctcacat gttctttccg gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3241 ttgagtgagc tgacaccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg
     3301 aggaagcgga agaggggtct gacgctcagt ggaacgaaaa ctcacgttaa gcaacgtttt
     3361 ctgcctctga cgcctctttt aatggtctca gatgtccttt ggtcaccagt tctgccagcg
     3421 tgaaggaata atggccgagc atattgatat gtccgtggca aagcggggag aggcgtgcga
     3481 tatcttcatc attcagtgtt tcaccctgcg cccggagatg atccagggct gcctgcatat
     3541 aaatagtgtt ccataacacg acggcgttag tgaccagccc cagtgtgccc agttgatctt
     3601 cctgaccgtc ggtatatcgt tttcttatct cacctttttg accgtgacag atggctctgg
     3661 caacggcatg gcgactttct ccccgattaa gctgggtcag aatgcgccgg cggtaatctt
     3721 catcatcaat ataattaagc agatacagcg ttttgttgat gcgccccact tcaatgattg
     3781 cctgagtcag tccggaagga cgttcacttt tcagcaatga acggaccagc actgaaacct
     3841 gtactttgcc cagcttcagg gagccagcgg tccggatcat ttcgtccctc tgaaggacta
     3901 tttttcgggg atctgattgc cctctggcaa tatcattcag cacgccatag tcggcatcat
     3961 ggtccattcg ccagaaaacc gaagcaccgg catcagccag gcgtggagaa aactggtatc
     4021 ccagcagcca gaaaaggcca aagacaagtt cgctggcacc tgctgtatcg gtcataattt
     4081 cggttggatt cagcccggtc tcctgttcca gaagaccttc cagcacaaag atagagtccc
     4141 tcagcgtccc cggtataacg atgccatgaa agccggaata ctgatcggac acaaagttgt
     4201 accaggtgat ccctctgtta ttaccaaagt atttgcggtt cggtccggca ttgattgttc
     4261 tgactggcgt aacaaagcgc attccatctg cagatgccac ttctcctcca ccccatatct
     4321 gtgccagtgg cagcgttgcc tgaaaatcaa ccagtctggc attagcgctg gtgatagttt
     4381 cagcccgcag atagttcgct tttgtccagt tcagccggtg tcgggtcagt gcaggaacat
     4441 ttgatctgat cagtggttcc agaccgatat tgcaggcttc agccatcagc acggcgctga
     4501 tgctgacggg cagatcatca actctggcac tggcttcact agcatggaaa aactcatcag
     4561 caaatccggt atgggcgtta atttcgagca gcaactccgt taaatccacc ggagggagta
     4621 gatcactgat cattttgctc agtcgtttca gactgtccgg ctcatcaaga ctggcgaggg
     4681 gagaaattgt caaccggggc ttcgggccag aaacatcgag ttcgacagcc tcattttcgc
     4741 aaagacgtgc agcaacctgt ctgtaacgac tatcaagctg atgacccaga gattttattg
     4801 cttcctgcgg gtctgtcggg tgccccaaag aacgataaac cttaatccgg tttgcctgcc
     4861 agtcagcacc ctgtagtaat cttgcacgag gatctcccca ccggttactg ccggtaacgt
     4921 agacatccct ccgcctcaga ctatcctgca gtttactgag aaagcagagc gtgtatcccc
     4981 tgcgggtgat atgtttttcc ttgttaatca ccagccgttt ccatgaccga ctgataattt
     5041 ccgttggtgc gtcgtcaaaa aactgccgcc gtgagctgaa ctcccggctg aggtagtcac
     5101 aggcattcag agtggtaacc ccggcaggtg cggatgaaaa tttaacggta ttcagcagat
     5161 ggggcaggaa acgacgaacg cgcccgtact gctccaccat ttcttcatga aaattatcgt
     5221 ctgagggccg ggcaatttca cggacaagcg tgatgatttc agccagcttt tgccttggga
     5281 tgtagctgaa cacctcagca cgaatcgatt cgtccggtgt ttcttctttc agcaggtacg
     5341 aacatgcgct ggcgagcgcc aatgcagatt tatccagatc cttcagcgag cggagccgtt
     5401 ttttctgccc aatctttctg gcgtcacgga tgataacggc cagcatggcg tccagaacgt
     5461 ccaatgcatc atccagcgcc agcgtttccc atgcaaggac aaaggcaacc agaaccgcca
     5521 tccttttctg cggtgacatc ctggcaatat tgaacaccga agtcatacca gcataacgtg
     5581 cgagattttt caggcgcaca gccgggagtg tactcaggtt ttcagcatgc aggccaaaat
     5641 cgttcagagt tttccagcgt tcaattgctt cattaaacgc cggaccactg atggtcacag
     5701 ggcccttttt cagtgattcc agtaaagaca ggcggctgca atcagttggc cccagcagca
     5761 tctccagctg tgaacgctgt tcggctgacg gtatcagtgc cagtttgttc cacaggcgca
     5821 acgtcgcctt ttcccttacc tctgaaatca accgggtcag cgtagtggct ccggggagaa
     5881 taatacgatg ttgcataagc caccctgtcg ccagatcgaa aagcaggcca ggacgttcgt
     5941 tgcttatcca gctccgggta tataaaagac gggtaaggcg aaatgtccag ggccaggcaa
     6001 attcacgata ctgatagtgc tgacgtatca gcgctgcatg ctcacggcgg gtattttccc
     6061 tctgaccgta ttctgcaaga acggtgatat cacgaatccc gagctgtctg gcggtaaaat
     6121 gccggacgcc ggaaggaata tgattcatat cggtgaggaa ggtgcccaga aaacggacac
     6181 atccaatttg cagggcaatg cccagacggt tgtgatcacc tctgcttttt ccgataaatt
     6241 ccttgtctgc ttcatcaagg tgaaaatatc gggcactgtt gcaaatagtc ggtggtgata
     6301 aacttatcat ccccttttgc tgatggagct gcacatgaac ccattcaaag gccggcattt
     6361 tcagcgtgac atcattctgt gggccgtacg ctggtactgc aaatacggca tcagttaccg
     6421 tgagctgcag gagatgctgg ctgaacgcgg agtgaatgtc gatcactcca cgatttaccg
     6481 ctgggttcag cgttatgcgc ctgaaatgga aaaacggctg cgctggtact ggcgtaaccc
     6541 ttccgatctt tgcccgtggc acatggatga aacctacgtg aaggtcaatg gccgctgggc
     6601 gtatctgtac cgggccgtcg acagccgggg ccgcactgtc gatttttatc tctcctcccg
     6661 tcgtaacagc aaagctgcat accggtttct gggtaaaatc ctcaacaacg tgaagaagtg
     6721 gcagatccca cgattcatca acacggataa agcgcccgcc tatggtcgcg cgcttgctct
     6781 gctcaaacgc gaaggccggt gcccgtctga cgttgaacac cgacagatta agtaccggaa
     6841 caacgtgatt gaatgcgatc atggcaaact gaaacggata atcaacgcca cgctgggatt
     6901 taaatccatg aagacggctt acgccaccat caaaggtatt gaggtgatgc gtgcactacg
     6961 caaaggccag gcctcagcat tttattatgg tgatcccctg ggcgaaatgc gcctggtaag
     7021 cagagttttt gaaatgtaag gcctttgaat aagacaaaag gctgcctcat
//
LOCUS       DAJOSY010000162         7042 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJOSY010000162 DAJOSY010000000
VERSION     DAJOSY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.162"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(166..1347)
                     /locus_tag="OHN27_004047"
     CDS             complement(166..1347)
                     /locus_tag="OHN27_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCQ2288393.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            1602..2831
                     /locus_tag="OHN27_004048"
     CDS             1602..2831
                     /locus_tag="OHN27_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCQ2288394.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            3291..3923
                     /locus_tag="OHN27_004049"
     CDS             3291..3923
                     /locus_tag="OHN27_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2288395.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            4257..5066
                     /locus_tag="OHN27_004050"
     CDS             4257..5066
                     /locus_tag="OHN27_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCQ2288396.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            5059..5541
                     /locus_tag="OHN27_004051"
     CDS             5059..5541
                     /locus_tag="OHN27_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288397.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(5574..5654)
                     /locus_tag="OHN27_004052"
     CDS             complement(5574..5654)
                     /locus_tag="OHN27_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288398.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(5690..6115)
                     /locus_tag="OHN27_004053"
     CDS             complement(5690..6115)
                     /locus_tag="OHN27_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288399.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAENVLTQTT
                     SWTLKSRYVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            6545..6991
                     /locus_tag="OHN27_004054"
     CDS             6545..6991
                     /locus_tag="OHN27_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288400.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYKFSITDDLMSMAVNELIKLT
                     KIENNVDLNKFTTICTDVLSPLVTRHNKEKNKRHSTLLKNPLFNFH"
BASE COUNT         2217 a         1439 c         1325 g         2061 t
ORIGIN      
        1 gtttcatttt taagttaagg attaaaaaaa gtgcagtgtc gatcacgaat atgtcatgag
       61 aatggaaaaa tgagggagcc cagaaaatgt atgtcgactt gccagccatc attattcaac
      121 agattaatga cggcagatat ttatcggtaa ggaggaaatg cagagctatt cgtcacgttc
      181 aatggtcaat gccacaccct ggcccccgcc gatacaaagc gttgccagtc ctttgcgggc
      241 attacgtttc accatttcat gaaccagaga aaccaggatt cggcaaccgg aagcgcctat
      301 cgggtgacca agtgcgatcg caccaccatt gacattgacc cgacgctcat cccactcaag
      361 catcttgcca accgaaagcg cctgtgcagc aaacgcttca ttagcctcga taagatcgac
      421 atcagccaac tgccagccta cacgctccag gcaacggcgg gtcgcataca ccggcgcaat
      481 tcccatcaat gccggatcta caccaacgct ggcaaatgcg cgaatgcggg ccagcacggg
      541 taaattcaac gctcgtgctt tggcttcgct catcatcatt accgcagctg cgccatcgtt
      601 tatggatgat gcattacccg ctgtcacaga accgagacta tcaaatgaag gatttaaacg
      661 ggctaaacct tctgcactgg cgtcagtgcg tggctgttca tcggtatcaa caaccaacgt
      721 ctgaccgtta ctttgggtca ttaccgggac gatctcatct ttaaatcgtc cggcgtcaat
      781 cgccgctcgc gctttttgtt gcgagctaag tgcgtaagca tcctgcaatt gacggctgat
      841 gccatattcg cgagccagat tttcggcggt gacaccaata tgataatcat tgaaggcatc
      901 ccataaccca tcatgcacaa gactgtcaac cagctggcta ttgccaagct gtgcaccggt
      961 gcggctatca gtcagaacat gtggtgcgcg gctcatgttt tcctggccac cggcgatgac
     1021 aatatcagcc tcgccacact gtatcgcctg agtagccaga tgcagtgctt taagtccgga
     1081 accgcagacg tcattaatcg tgattgcaga aacgctatta ggcagaccac ctttaatagc
     1141 cgattgcctt gccggattct gccctgcccc tgcagtcaac acctgaccaa gaattacttc
     1201 atccaccgca tatgcaggaa cgccggtacg ttctattaac gctttcacga ccagactacc
     1261 aagttccacg gcggaatgac ccgctaacgc accgcgaaag cagccgatag gtgtccgtaa
     1321 cgccccgaca atcacaacgt ctttcatcac aacctcgcac caattaacag tgcaatagta
     1381 aatgattgtt aattacagtt atcttaattg tttaaaaaaa gtgattttta tcacaaagga
     1441 aatatgcctg agcagtagtc agagtcataa ctggcacgta aggtttgcaa ccactaaccc
     1501 accaatagag gggtagatag ggcgttaatc tcccatactt aacctggttt atggtaaatt
     1561 gccctccatt ttgtttaatt tgtagatgat acgttcagat aatgtctaat atttggtcta
     1621 aagaagaaac tctgtggagt ttcgcgctct acggcacagc cgttggtgca ggcacgctct
     1681 tccttcctat tcagttaggt tcggcagggg ctgtggtcct gtttattact gctctggtcg
     1741 cctggccttt aacatattgg ccacataaag ccttatgcca gttcatcctc tcatcgaaaa
     1801 catcagcagg tgaagggata acgggcgcag taacacacta ctatggcaag aagattggta
     1861 atctgattac cacgctgtac ttcatcgcct tttttgtcgt cgtgttgata tatgcagtgg
     1921 caattaccaa ctcacttacg gaacagctgg caaagcatat ggttattgat cttcgcatcc
     1981 gtatgttggt gagtctgggt gttgtattaa tcctgaatct catttttctg atgggacgcc
     2041 atgcaaccat tcgggtaatg ggttttttgg tattcccatt gattgcctat ttcttatttc
     2101 tttccattta ccttgtcggt agttggcaac ctgatctatt aacgacccag gtagagttca
     2161 atcagaatac ccttcaccag atatggatat cgattcccgt gatggttttc gcctttagcc
     2221 atacgcccat tatttctacg tttgccatag acagacgtga aaaatatggc gaacacgcta
     2281 tggataaatg caaaaaaatt atgaaagtcg cttatctcat catctgcata agtgtactgt
     2341 tctttgtctt tagctgcctg ctttctattc caccttcgta tattgaagcg gctaaagaag
     2401 aaggggtcac cattttatcg gcgctttcta tgctgccgaa cgccccagca tggctgtcaa
     2461 tttccgggat tattgtcgca gtagttgcga tgtcgaaatc attcctgggt acgtactttg
     2521 gcgttattga aggtgccacg gaggtcgtca aaacaacact acagcaggtt ggtgtaaaga
     2581 aaagtcgtgc atttaaccgc gcactatcaa ttatgttggt atcgctgatc accttcattg
     2641 tttgttgcat taacccgaac gcgatttcga tgatttacgc gatcagcggc ccgctcattg
     2701 ccatgatact tttcatcatg cctacgttgt caacgtatct gatcccggcg cttaaaccct
     2761 ggcgttccat cggaaatctg attacgctga tcgtgggtat cctgtgcgta tcggtaatgt
     2821 tctttagctg atatccattg tgaatggttc aaaattgcta caattttgaa ccattccagt
     2881 tttcataatg ttcattcgtt attattccgc actatcactt ccctcatcat atcctagtat
     2941 tatcagttag ttaaacacac aaagagatct gaacatctta aataagacta tttaagatgc
     3001 ataacttaga ttcgcaagat atcttccggt cattcaaaaa caatttgcga tatagatatt
     3061 ctcataccat gagatagtta tctgaaccac cctcaaccag gtaagattaa atcagtgaaa
     3121 cataatcata tttaataacg agaaatgcat tttaaaatgt cttaatcaga attatagcaa
     3181 atacagatta atcgcaattt ttcccagcaa aattacaaaa tcatggtgta ttgacatcat
     3241 caatcaccaa tgaaattatg caattatata cggataggga ggttcttaac atggggcttt
     3301 gtagtcgtta taaaagtctt acatgcaata gttgctcaat gcattgccaa ataatgccag
     3361 aagagtcacc gcgtttgcag tactgcgcaa actcgtgttt ttgtatgtgg cccgaagaaa
     3421 gttcatattt taatcgtggt gtggtagaag gtatcttaac aaaaaaccac aatgcgagat
     3481 taagcggata tatttttgtc gatttttcag taagtttttt acgtctattc cttgaaaaag
     3541 actggattga ctatcttgca agtactgaca tggggattgt tttagtcagc gaccgcaata
     3601 tgcaatcgtt agctaattac tggcgaaaac acaactcagc aatatctgct gtcatatata
     3661 atgatgatgg tcttgatgtt gccaatgaaa agatcagaca actatttatt ggtcgttatt
     3721 tatcatttac cagaggtaac acattaactc agatggaatt caccataatg gggtatatgg
     3781 tttctggtta taatccatat cagattgctg aagttctgga tatggatatc cgtagcatct
     3841 acgcgtacaa gcaacgaatc gaaaagagaa tgggtggtaa aataaacgaa ttatttattc
     3901 gttcacattc ggtccaacat tgatactcaa cgaccagcca gaatcatact ctgtttatac
     3961 gggcatttat tgaaatgtcc gtattttata aataggccgt cccctcaaga gtaaacacca
     4021 tagtatttaa aaccatcaca cgaaaaattc agaagcatta cgaaatatga atttacaaac
     4081 aatagtggca taaatgttaa ccatgttaat ttacgtaaag ttttacgttg caacattaaa
     4141 gcctcatttc aatcatcatg ataaatataa aattaatata tatttatgcc gtaaatacca
     4201 atatacttag caaactatgt gatctccatt tcgattgatt tagtgtttat tgacgtatgt
     4261 actggattat taacgataat atcgagttct ggcctgagca ccgaaaatta atatcggtac
     4321 ataacgccga tcttaacgtc gttctgacaa cgccagccag tcgatgttta tcacttctac
     4381 ttgaagcttt tcctgatgtg gttgcacaac aagatttttt caccagagtc tgggaagaag
     4441 aaggtatgcg tgtgcctact aacacgttat atcagaacat atccattatc agacgcggat
     4501 ttcgcgctgt tggtgatact acccactcgc taattgcaac cgtgccgaga agaggattca
     4561 agatccataa tgacattaac atacaaaatc atgtaataaa ctcgtcaaca gacgcacata
     4621 cacacaatgc cccacctgcc ataaaagtta atgcgggata caaagagagc attggtggcg
     4681 caaagaattt caataacaaa atcctcaaac atataaaaag tcatctaatt atgttgagcg
     4741 catttgtcat aggcgcatat tctgcatatt ggctatggaa taataatcaa ccgaagccat
     4801 ttttcaagga ttacaagact gttgcggaaa ttaatggctg ccattttaat gtaacagaag
     4861 atacaattga tgggttgaaa gagttcgata aatataaaac acggatactg gattcgggga
     4921 ttaactgtaa aaaacatccg tggttatact tccctcttgc aaaatcctca ccggggatga
     4981 ttgttatggc atgcaataaa aactataacc aacatgaagt ggcagactgt ttaaccctgt
     5041 cttaccgtga ggttaatcgt gattgattat aaaaaaaact tattatttat tttagttttt
     5101 atcagtgggt ttattctctt cactgtctat tcttacactg cagaaaaaat gatatataac
     5161 gaaacatgca ctgcaaattg ggtaattttt aatgaccagg gacgagcaaa tcttaccatc
     5221 gactttatgt ataacaaaaa aaataaaaca ggaactgttg cgttgagtgg aacctggcaa
     5281 caaggtaaca gagaaagtaa atcaatacgg cgaaatattg aatacacatg gattgaaaac
     5341 tatgacacag cccatttaac atcaaagaaa gttaataagt ttgagattat ggatcaagtt
     5401 gatgatgata gacttgcaca acttattcct gatttttatg tctttccaga aaaaagtgta
     5461 agctataata tactaaagca aggtaagcat gcttttattt tgagcattgg taacagagca
     5521 ataatgcatt gtgcaaggta aactaaaata gataacaaaa agatggaacc tcgttaatca
     5581 taaaacaata aaattaaata ttctcgcagt atatggcagt ctaaagcatc aaagttttga
     5641 tcaacatctt tcattttaga catctccttg caatgtaaaa tatcatgtat caacaacatc
     5701 tggtgcaaat ccatgagtcg aactcgacat tcatcttagc tcgattatta ttccttcgta
     5761 cagtcgatgt aaacaataca gaaagaggat tattaagaac cagtttactc agtgaatcaa
     5821 tagaggaaag gactaacgtt tctttaaaag aattgatttc atcatctgtt aaactaaact
     5881 catcattgac agatcgtgag atataactgt ttttaacttt actctttacg ttgactttat
     5941 tgacagaatt aacattcaca tatcttgatt ttaatgtcca tgatgttgtt tgcgtgagaa
     6001 cattctcagc attaaggaat ttttttaacg gaatccttgg tttctttttc gagtccgaat
     6061 ttacaatatc atgcatatag accatttcat gaatctgcga aaattcaatg tccattgtat
     6121 acctcacatt tttaccgtga ctcgatgtta ctgttcaata atcaccttcc atcaatacta
     6181 aaattaatac ccctaatgtg ccgataacaa atatagtcat tctacgtaac gtctccataa
     6241 ggtgataacc ttgatcaaat gaatttcgaa tataataaag gaaatctttt gagatgttta
     6301 tcacgaataa agctatttat gttaccaata tcatttcgag tgcgcacact atctatctgt
     6361 tttttcgaga tataaaaaac aacgtgtaag ttatgtaaat tgccgatgct acttagtaac
     6421 attatggtaa aaaaagtaaa aatgagcgat ttttgataga ataaagcatg agaaaaggtg
     6481 atatttgaca ttatcagaag ctgcgaattc ggattttgct ctaatcaaat gagagaaatg
     6541 taaaatgaag ccacgaaata ttaataatag cctaccactg caaccattag ttcctgatca
     6601 ggagaacaaa aataagaaaa atgaagagaa atccgttaat ccagttaaaa tcacaatggg
     6661 gtctggttta aattatattg aacaagaatc tcttggagga aaatatctaa cacatgattt
     6721 gtcaataaag atagcggata tttctgaaga gataattcag caagcaatat tatctgctat
     6781 gagcatatat aaattttcga taacagatga tttaatgagt atggctgtaa atgaactcat
     6841 aaaactgacc aaaatagaga ataatgtaga cctgaataaa ttcactacta tatgcacaga
     6901 cgttctatcc cccctcgtca ccagacataa taaagaaaaa aacaaacgac attctaccct
     6961 tctcaaaaat ccccttttta attttcattg aaaaaatggc aattgatact atgaatccag
     7021 gtcatacaca ttaaggcatt ta
//
LOCUS       DAJOSY010000163         6935 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJOSY010000163 DAJOSY010000000
VERSION     DAJOSY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.163"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(23..328)
                     /locus_tag="OHN27_004055"
                     /pseudo
     CDS             complement(23..328)
                     /locus_tag="OHN27_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(404..1195)
                     /locus_tag="OHN27_004056"
     CDS             complement(404..1195)
                     /locus_tag="OHN27_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCQ2288401.1"
                     /translation="MSEKLKIVYRPLQELSPYAHNARTHSTEQVAQLVESIKQFGWTN
                     PVLIDEKGEIIAGHGRVMAAEMLKMDSVPVIVLSGLTDEQKKAYRLADNRLPMNAGWD
                     EDLLRMELSDLINADFDVSLTGFIPTEIDELLTDVLPGTGNEEEPYTTKIDTPVYEPS
                     GGKPDISELYDDTKTQELISRIRSASLEPDIEKFLLCAAERHTVFNFSRIADYYAHAP
                     SEIQCFFEESALVIIDYQQAIENGFVRMTQRMVEIMHGGEEEEYA"
     gene            complement(1333..2790)
                     /gene="trkG"
                     /locus_tag="OHN27_004057"
     CDS             complement(1333..2790)
                     /gene="trkG"
                     /locus_tag="OHN27_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkG"
                     /protein_id="HCQ2288402.1"
                     /translation="MNTSHVRVVTHMCGFLVWLYSLSMLPPMVVALFYKEKSLFVFFI
                     TFVIFFCIGGGAWYTTKKSGIQLRTRDGFIIIVMFWILFSVISAFPLWIDSELNLTFI
                     DALFEGVSGITTTGATVIDDVSSLPRAYLYYRSQLNFIGGLGVIVLAVAVLPLLGIGG
                     AKLYQSEMPGPFKDDKLTPRLADTSRTLWITYSLLGIACIVCYRLAGMPLFDAICHGI
                     STVSLGGFSTHSESIGYFNNYLVELVAGSFSLLSAFNFTLWYIVISRKTIKPLIRDIE
                     LRFFLLIALGVIIVTSFQVWHIGMYDLHGSFIHSFFLASSMLTDNGLATQDYASWPTH
                     TIVFLLLSSFFGGCIGSTCGGIKSLRFLILFKQSKHEINQLSHPRALLSVNVGGKIVT
                     DRVMRSVWSFFFLYTLFTVFFILVLNGMGYDFLTSFATVAACINNMGLGFGATASSFG
                     VLNDIAKCLMCIAMILGRLEIYPVIILFSGFFWRS"
     gene            complement(2987..3172)
                     /gene="rzoD"
                     /locus_tag="OHN27_004058"
     CDS             complement(2987..3172)
                     /gene="rzoD"
                     /locus_tag="OHN27_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HCQ2288403.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSGNDW"
     gene            complement(3389..3865)
                     /locus_tag="OHN27_004059"
     CDS             complement(3389..3865)
                     /locus_tag="OHN27_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCQ2288404.1"
                     /translation="MQTLNSQRKAFLDMVAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(3869..4210)
                     /locus_tag="OHN27_004060"
     CDS             complement(3869..4210)
                     /locus_tag="OHN27_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCQ2288405.1"
                     /translation="MKMHNAPHSWPDLLELLQSWWRGDTPLGAVVMSIIMAGLRIAYF
                     GGGGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMR
                     VIGNKFGAHKE"
     gene            4287..4589
                     /locus_tag="OHN27_004061"
     CDS             4287..4589
                     /locus_tag="OHN27_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288406.1"
                     /translation="MFPEPNNEKAPGHAGVRWSARLSFWRYILVFDIVKSPKVTIQTK
                     RIFMSEKNKPQDENKPQQPVAPKPTPTQSTTDFATRRVFVGDSADSVIEHIKKQPR"
     gene            complement(4558..5127)
                     /locus_tag="OHN27_004062"
     CDS             complement(4558..5127)
                     /locus_tag="OHN27_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288407.1"
                     /translation="MRELPKDYFLGVDDELVDYLEKQGEETIREIHLSNKTNVENGYK
                     LLNIQIVGIGSSFLLLTQKTNFDFLTAGITTFTLLWTWCAIYLVCTGLSVKVRGLINA
                     PPDHLYHEKYKDMEPSSFKIFADAGYLGPDKLLPLIRRYRLVDLSDTARELLLENEKI
                     RTSLDKARMYTILAPVAAMFISAVFLYVQ"
     gene            complement(5349..5891)
                     /locus_tag="OHN27_004063"
     CDS             complement(5349..5891)
                     /locus_tag="OHN27_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCQ2288408.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKIA"
     gene            complement(5888..6178)
                     /locus_tag="OHN27_004064"
     CDS             complement(5888..6178)
                     /locus_tag="OHN27_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCQ2288409.1"
                     /translation="MVNLRKAAKGQMCQIRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(6178..6777)
                     /locus_tag="OHN27_004065"
     CDS             complement(6178..6777)
                     /locus_tag="OHN27_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCQ2288410.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSQEEVENVAAQLLEFA"
BASE COUNT         2017 a         1736 c         1390 g         1792 t
ORIGIN      
        1 cgcccgttct gggttataaa attcagctga tactgatacc tgctgtggat tttttcatca
       61 ccacaaccag cagatcgctg atactggttg ttggtgtcca gttattcgct cctgatgaag
      121 atacggtgaa tgtcagtgtc agcgtcccct gtccggcagg catatctata acggaggaaa
      181 atacgccctg agcatccgtc gtggactgat taaaaatctc ctgaccattg cgggtcactc
      241 ttaaccggca ggttgaatac cagtatgact gttggttatt actgttgaaa ttctcatgct
      301 taccaccgcg gaataacact ggcggtagcc agcacgacgc aacgtccggt aagtcagaac
      361 tttgtccggt cgcccgtgag tcagaataaa ggcgcaaaaa tcatcacgca tattcctcct
      421 cctcaccacc atgcatgatc tccaccatgc gctgcgtcat ccggacaaat ccattttcaa
      481 tagcctgctg ataatcaatg atcaccagcg ccgactcctc gaaaaaacac tgaatttcag
      541 agggggcgtg agcgtaatag tccgcaattc tgctgaaatt aaacaccgtg tgacgttctg
      601 ccgcacacag gaggaatttc tcaatatcag gctcaaggga cgccgaacgt atccggctga
      661 tcagctcctg agttttcgta tcgtcgtaca gttcactgat atccggttta ccgcccgacg
      721 gctcataaac aggcgtatca attttcgtcg tatacggctc ctcctcattt cctgtaccgg
      781 gcaaaacatc cgtcaacagt tcatcaattt ctgtcgggat gaagcctgtc agggagacat
      841 caaaatcagc attgattagg tccgacagct ccatccgcaa cagatcttca tcccagccag
      901 cattcatcgg caggcgatta tctgccaggc ggtacgcctt tttctgctca tccgtcaggc
      961 cagacagaac aatgaccgga acagaatcca ttttgagcat ttcagccgcc ataacacgac
     1021 cgtgacccgc aataatttcg cccttttcgt caatcagcac cggattagtc cagccgaatt
     1081 gcttaatact ttctaccagt tgtgccacct gctcagtact gtgcgtcctg gcgttgtgcg
     1141 catacggtga caattcttgt aatgggcgat agactatctt taatttctcg ctcatacagc
     1201 cttgctttat gaataaaacg caccccagca gccagtgcta ctggggacgg aggtgttgct
     1261 ggtaaagtta ggtattggat caatgagtga gtcaacataa tattaaactc acaattataa
     1321 atcagccata tattaggagc gccaaaaaaa acctgaaaac aatataataa caggataaat
     1381 ttcaaggcga ccaagaatca tagctatgca cattaagcat tttgcaatgt cattaagcac
     1441 tccgaatgac gatgcagtag ccccaaaacc taatcccata ttattaatac atgcagccac
     1501 tgttgcaaat gatgtaagaa aatcatatcc cataccattt aacaccagta taaaaaacac
     1561 cgtgaagaga gtataaagaa aaaagaaact ccatacagac ctcattacac gatctgtaac
     1621 tatcttccct cctacattta cactcaacaa cgctctggga tgagaaagct gatttatctc
     1681 gtgtttgctt tgtttgaaaa gtataagaaa tcgaagtgac ttaattccac cacaagttga
     1741 acctatacat cccccaaaga aacttgacaa cagcaaaaac actatcgtgt gcgtgggcca
     1801 acttgcataa tcctgcgtag ctaaaccatt atcagtgagc atggagctgg caagaaaaaa
     1861 cgaatgaata aaacttccat gcaagtcata catacctata tgccagacct ggaaagaggt
     1921 aacaatgatc acccctaagg ctattaacag aaagaaacga agttcaatat ctctgattaa
     1981 aggttttatc gttttcctgc taataacaat ataccaaaga gtgaagttga aagccgatag
     2041 cagggaaaaa gaaccagcca ccagctcaac caaatagtta ttaaaatatc cgatactctc
     2101 gctatgagtt gagaaaccac caagcgaaac tgtggaaatc ccgtgacaaa tagcatcaaa
     2161 caaaggcatt cctgcaagtc tataacagac aatacaagca atacctaata aagaataagt
     2221 tatccacagt gtccgtgacg tatcggccag gcggggagtg agtttgtcat ccttaaatgg
     2281 ccccggcatt tctgactgat aaagctttgc accaccaata cccaataatg gcaatacagc
     2341 aaccgccaga acaataactc ctaaaccacc tataaaattt aactgtgacc gatagtacaa
     2401 atatgcccga ggtaatgaac taacatcatc aattacagtt gctcctgttg ttgttattcc
     2461 agaaacccct tcaaacagag catcaatgaa cgttaaatta agttctgagt caatccataa
     2521 agggaatgca ctaataacag aaaacaaaat ccaaaacatt acaattataa taaacccatc
     2581 acgggtacgt aattgaatgc cagatttctt agttgtatac cacgctccgc caccaatgca
     2641 aaaaaatata acgaaagtta taaagaaaac aaacaggctt ttttctttat aaaacaatgc
     2701 tacaaccatt ggtggcaaca ttgaaagact atagagccaa accaggaacc cacacatatg
     2761 agtaacaact cttacatgag atgtattcat atctaaatat tctttcaatt ataaccacct
     2821 tgctgcaata ttatgattat actgtataaa atttaactcc tcttagatct tacttcactg
     2881 ttccttatga aacaatcatc aaaatgaatc atattgtagt taagatttta ctttaaacac
     2941 tgctcggtta tgtattgctg agcaccttca agttgggcct gcatcattac cagtcgttcc
     3001 cggagggtga aataatcccg ttcagcggtg tctgccagtc ggggggaggc tgcattatcc
     3061 acgccggagg cggtggtggc ttcacgcact gactgacaga ctgctttgat gtgcaaccga
     3121 cgacgaccag cggcaacatc atcacgcaga gcatcatttt cagctttcgc atcagctaac
     3181 tccttcgtgt attttgcatc gagcgcagca acatcacgct gacgcatctg catctcagta
     3241 attgccgcgt tcgccagctt cagttctctg gcatttttgt cgcgctgttc tttgtaggcg
     3301 atggcgttat cacggtaatg attaacaccc catgacaggc agacgacgat gcagataacc
     3361 agagcggaga taatcgcggt tactctgctc atacctcaat ctctctgacc gttccgccag
     3421 cttctttgaa ttttgcaatc agactgtcag ccttatgctc gaactgacca taaccagcgc
     3481 ccggcagtga agcccatata ttgctgcaac ggtcgatagc ctgacggata tcaccgcgat
     3541 caatcatcgg caaagcgcca cgctctttaa tctgctgcag cgctacagca tcctgacttt
     3601 ctggagaaaa atctttcagg ccaagttgct tgcggtaggc atcccaccag cgtgaaagaa
     3661 gctggtaacg tccagcggct gttgatttga gtttcggatt tagcgtgaca agtttgcggg
     3721 ggtgatcgga gtaatcagtg aacagttcgc caccgacaat aacatcataa ccgtgatttc
     3781 tggttttctg ccgtccgtta tctgttcctt ctgaccatgc caccatatca aggaaagctt
     3841 tacgctggga attaagtgtc tgcattaatt actccttatg ggcaccgaac ttgttaccga
     3901 tgaccctcat tgccgcacca cgaatagcat caacaccaat cagccccacc ccaccaccaa
     3961 tggcaacaga aagtgattta ggccatccga catactcaag cgcggatgca aaggtcagcg
     4021 tcagagcacc acaaagcaga atctcaagcg tttttcgttt ccagccgccg ccaccgccaa
     4081 aataggcaat gcgcaaacca gccataataa ttgacataac cactgcaccc agcggcgtat
     4141 ctccacgcca ccaactttgt aagagttcca gtaagtcagg ccaggaatga ggggcattgt
     4201 gcattttcat aagcctcacc tccgagagtt cggatggtgc taagtgtaag attcaggctc
     4261 tcaggcttgc taacaagaag tcgaggatgt ttccggagcc taacaacgaa aaagccccgg
     4321 gacatgccgg ggtcagatgg agtgccagat taagcttctg gcggtatata ctcgtgtttg
     4381 atatcgttaa atcgccaaaa gtaacaattc aaacaaagag gatttttatg tctgaaaaaa
     4441 acaagccaca agatgaaaat aaacctcagc aacccgtggc accaaaacca actccaacac
     4501 aaagtactac agactttgct acacgtcgtg tttttgttgg agattctgcc gactcagtca
     4561 ttgaacatat aaaaaaacag ccgagataaa catcgccgct accggagcaa ggatggtata
     4621 catccttgct ttatcgagac tcgtgcggat tttctcattt tccaacagta actctcttgc
     4681 tgtatcactc aagtcaacaa ggcgatacct tcgtataagc ggcaataact tatcaggtcc
     4741 taaatatcct gcatcagcga atattttaaa gctcgagggc tccatatcct tatatttttc
     4801 atgatataga tgatcaggag gggcattgat caggccccta accttcacag ataaaccagt
     4861 acataccaag taaatggcgc accatgtcca tagtaatgta aatgtggtaa ttccggcggt
     4921 gagaaaatcg aaattagttt tctgtgtcag caataaaaaa gatgaaccaa ttccaacaat
     4981 ctgaatgttc agaagtttgt atccattctc aacattggtt ttgttagaaa gatgaatctc
     5041 tcgtatcgtc tcttcccctt gtttttcaag ataatcgacg agctcatcat ctactcctaa
     5101 aaaataatct ttaggtagtt ctctcatctc acctccacat cctgtactga aaacaatttt
     5161 accagaatgt ctcgattcta ggtattccgc caggaatcgc gctccagaaa tgaaacatca
     5221 ggttcgccag taccagaaac aacaaaaccc gctcaatggc gggttctggt aaagttcatg
     5281 cgcttggttc gcctcgcgat acagctttgc gaagcatacc ggaattgaag cagtttatgc
     5341 gtaaaaaatc aagctatttt ttgagcaaat gattctcgca tgggaatata tagggcatac
     5401 tcagcaacag ccaaccaatt agcaattcgc ttttcgcatg tgctaaaaca ccactcagga
     5461 tgtgcatcat ttagcaattc agccattttg cgcttagtca tcccccgtcc ttcataccgt
     5521 tgccggagga cactaatcaa tccaggatgc tctgccagca cctcacttat gactctatca
     5581 atacataacg cctctgcatc agtacaatgc gccagccagg tcttttgctt gccattgatc
     5641 atctctcgca aaaacgcttc cagctcaggt ttctctattc ccgctttttt catcctgcgc
     5701 agggcttcat tgacggctgt tttcgtcaat tttttggatg ccagcaactg gttaaacata
     5761 tttcccgtct taccgccgcc aatatacgac cagcgccccc acatgcgcag ttttccctga
     5821 atccagacac tttccagcgt ggtgagacga aggtgttccc cgcttttgcc tgtatttgtt
     5881 gggtaaatca taaataacct tcctttctcc agatttcttg tgtgcgaaaa acaccttctg
     5941 catgcatcag gcgtaattct tctttggtgt aatcgctggt ttttacccgc ccgtcgatta
     6001 aatcgtggca tgagctacag gcaatcgctg cctgcatatc gtgtggtttt gtcgctgttc
     6061 cgcacgtccc cgccagcctg taatgcgcca gcacagaggt ttcgggattg tgattgcagt
     6121 agccagggat tctgatctgg cacatctggc ctttagccgc tttacgtaaa ttcaccatta
     6181 cgcaaactcc agtagttgtg cggccacatt ttcaacttcc tcctgagagg agaatttacg
     6241 gaacagaatc cagttccaca gcacattcag aacagattta taaacctgct gaaactcggt
     6301 ttcgtccata ttcgcaaacg cgatggattt cgccctgcgc ccacggctac cgtccggata
     6361 aatatgctcg gtgtaaaatc cggcctgaat ggttacccac tcgcggaaag cctcaaacga
     6421 ctttagcaat gccgtatccc gagttctgcg tgtcgcaact gtatttagat attgctctgc
     6481 ggcatcactc agggctggtg tgtgttcccg accaactgat tcgcacaagt aatccacgaa
     6541 accagacagc agttctcgtt cgcgaggtgt gatcgcccca ccgaccggag tccagtaatc
     6601 gaatcccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac gcacacgctt
     6661 aaagtctgcg tgtatccact cacctatttt gatttgatgc agaaaatcgc aactctccgg
     6721 cgtcgccggg agaagtaaac cagaagaggt ttgtttgacc agttgtatat gcgccatttc
     6781 tcaatctctc gatggcgcag cgcagcagat gccagttgtt caggctgacg aataaagtat
     6841 aaataaactg gctatggtgt aaagccccac atagcatgaa caaacactac atatcaaata
     6901 gctggtacaa ggatagaaat acgacattta ttatt
//
LOCUS       DAJOSY010000164         6879 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJOSY010000164 DAJOSY010000000
VERSION     DAJOSY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.164"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            296..1918
                     /locus_tag="OHN27_004066"
     CDS             296..1918
                     /locus_tag="OHN27_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCQ2288411.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     NNNYQFIVTINSKNYGCSLDNTDVNWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETMIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMNNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2284..3351
                     /locus_tag="OHN27_004067"
     CDS             2284..3351
                     /locus_tag="OHN27_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2288412.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3348..6083
                     /gene="rbbA"
                     /locus_tag="OHN27_004068"
     CDS             3348..6083
                     /gene="rbbA"
                     /locus_tag="OHN27_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCQ2288413.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYKPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6083..>6879
                     /locus_tag="OHN27_004069"
     CDS             6083..>6879
                     /locus_tag="OHN27_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCQ2288414.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVAL"
BASE COUNT         1700 a         1635 c         1859 g         1685 t
ORIGIN      
        1 ttcctcaggg cttagttgtt ttcaatctca ttaaaactcc atgaatacgg tggtatgtct
       61 ttttaatcct gccagtattt cataggtttt cttaatcgat tgtagaggca tgttctgatg
      121 acgcctctac aatctttagg gactttctct gctatgagat aagaaatttc atattatgct
      181 ttgaggctga atgatttgtg caaaatataa ttattttccc atcgaacatg aggtgtatgt
      241 cataagataa aatgttaact atgtattcct cgcggtaaat aatatgaggt aaaatatgaa
      301 aattggaact gtggcaggca ctaacgacag tacaacgaca atcgcaacaa atgatatggt
      361 gcaagagcat gttaccaact tcactaaaga actgtttggg tatattgcaa atggtatagg
      421 tgatgatata tcaagtattg ccaggacaat gctcggtgag gttgtggaaa aaatagatga
      481 ttggcaaata gaacggttcc aacaatctat tcaagatgac aaaatttcat ttaccataca
      541 aacggatcat tcagaaaaat atagtatgct atcgggcatg cgtgctcata ttctacgtag
      601 gaataataat tatcagttta ttgtgacaat taatagcaaa aattacggtt gctctcttga
      661 taataccgat gttaattggt gcagtattgt ttatttactt aataatatga cagtaaatga
      721 taatgctaat gatgttgcag ttactgagtc gtacaaaccc atctggaact ggaaaatatc
      781 gcaatataat gtatctgata ttaaattcga aacaatgata aaaccccagt tcgctgacag
      841 aatctatttc tccaattgtt cacccgttga tcccacaagt accagaccaa cctattttgg
      901 tgataccgat ggctcagtcg gtgcagtttt gtacgcgctt tttgcaacgg gacatttgag
      961 aatcatggct gaaggagaaa atttccttag ccagttgctt aatatagaag atgaagttct
     1021 caacgtattg ctaagggaga actttaatga gcaacttgac actaatgtta atacaataat
     1081 cagtattctg aacagaagag atatagtttt agaaagtctt cagccttatt tagttattaa
     1141 taaagacgct gtgacgccat gtacgtttct gggcgaccag actggcgatc gatttagtaa
     1201 tatctgtggc gatcaattta tcatcgattt attaaaacgc ataatgagta tcaatgaaaa
     1261 cgttcatgta ttagctggaa atcatgaaac gaattgcaat ggtaactata tgcagaactt
     1321 cacgcgcatg aaaccacttg atgaggatac ctactctggg ataaaagatt atcctgtatg
     1381 tttctatgac cccaaataca aaataatggc aaatcatcat gggataactt ttgatgatca
     1441 gcgaaaacgt tacattatag gaccgataac agtctctagt gatgaaatga ccaatgctct
     1501 cgatccggtc gaattagcgg caattatcaa taaaaagcac catgctgtaa ttaatggcaa
     1561 aaagtttaaa aatagccggg ctatctcttg tcgatccttt aatcgttatt tttctgtttc
     1621 gacagattac agacctaaac ttgaagcact attagcatgc tcacaaatgt taggtataaa
     1681 tcaagttgtt gcacataacg gtaatggtgg aagagaatgt ataggtgaaa cggggacagt
     1741 tttaggtctt aacgcccgtg acagtaaaca tgctggaaga atgttcagca tgaataattg
     1801 ccaaattaac cctggtgcag ggcctgagat aaccactccc tggaagtctt accagcatga
     1861 aaaaaacaga aatggactta tgcctttgat tagaaggcgc acaatgttac agctctgaac
     1921 catagacaac atgaatatta atgttactat ctggttgaga ctgttgataa aacgtaaaaa
     1981 ggaatgctct gttcctgcaa ctgaacaggc gtgaaacgcg atccaacagg atggataccg
     2041 ttttgtcagc cagttttata gatcacactt atgagtatgc gtgtttcatt tacggccagg
     2101 aatgctgatg atgacaacat tactacccgt tttcactaag ccttccccgt tggcgctcaa
     2161 tgctctgcgc gctggtcgta tttgtcgttt ccttcttatc ccggatggaa gaatccgctg
     2221 aacgtcgttc ggcggcagtt gtaacacgcg tcgtttgctt acagggatta tggcgggccg
     2281 attatggata agagtaagcg ccatctggcg tggtgggttg tcggggcact ggcggtggct
     2341 gccgtcgtgg cgtggtggct gttgcgcccg gcgggtgtgc cggaaggctt tgctgtcagc
     2401 aatgggcgca ttgaagcgac ggaagtggat atcgccagca aaattgccgg gcgtatcgac
     2461 accattctgg tgaaagaagg ccagtttgtt cgcgaaggtg aagtgctggc gaagatggat
     2521 actcgcgtgt tgcaggaaca gcgactggaa gccatcgcgc aaatcaaaga ggcacaaagc
     2581 gccgttgccg ccgcgcaggc tttgctggag caacgacaaa gcgaaactcg cgccgcgcag
     2641 tcgctggtta atcaacgcca ggcagaactg gactccgtag caaaacgtca tacgcgttcc
     2701 cgttcactgg cccaacgagg ggctatttct gcgcaacagc tggatgacga ccgcgccgcc
     2761 gctgagagcg cccgagctgc gctggaatca gcgaaagccc aggtttcggc ttctaaagcg
     2821 gctatagaag cagcacgcac caatatcatt caggcccaaa ctcgggtcga agcagcgcaa
     2881 gccactgaac ggcgcattgc cgcagatatc gatgacagcg aactgaaagc cccgcgtgac
     2941 ggacgcgtgc agtatcgggt tgccgagcca ggggaagtgc tggcggcagg cggtcgggtg
     3001 ctgaatatgg tcgatctcag cgacgtctat atgaccttct tcctgccaac cgaacaggcg
     3061 ggcacgctga aactgggtgg tgaagcccgg ctgatcctcg atgccgcgcc agatctgcgt
     3121 attccggcga ccatcagttt tgtcgccagc gtcgcccagt tcacgccaaa aaccgtcgaa
     3181 accagcgatg aacggctgaa actgatgttc cgcgtcaaag cgcgtatccc accggaatta
     3241 ctccagcagc atctggaata tgtcaaaacg ggcttgccgg gcgtagcgtg ggtgcgggtg
     3301 aatgaagaac ttccgtggcc tgacgacctg gtggtgaggt tgccgcaatg acgcatctgg
     3361 aactggttcc cgtcccgcct gtcgcgcaac tggcgggcgt gagccagcat tatggaaaaa
     3421 ccgttgcgct gaacaatatc actctcgata ttccggcccg ctgtatggtc gggctgattg
     3481 gcccggacgg cgtcgggaag tcgagtttgt tgtcgttgat ttccggtgct cgtgtcattg
     3541 agcagggcaa tgtgatggtg ctgggcggcg atatgcgcga cccgaagcat cgccgcgacg
     3601 tctgcccgcg catcgcctgg atgccgcagg ggctgggcaa aaacctctac cacaccttgt
     3661 cggtgtatga aaacgtcgat tttttcgccc gcctgttcgg tcacgacaaa gcggagcggg
     3721 aagtgcgaat taatgagctg ctgaccagca cggggttagc accgtttcgc gatcgtccgg
     3781 caggtaaact ctccggcggg atgaagcaaa aactggggct gtgctgtgcg ttaatccacg
     3841 acccggaact gttgattctt gatgagccaa caacgggggt tgacccgctc tcccgagccc
     3901 agttctggga tctgatcgac agtattcgcc agcggcagag caatatgagc gtgctggtcg
     3961 ccaccgccta tatggaagag gccgaacgct tcgactggct ggtagcgatg aatgccggag
     4021 aagtgctggc aaccggcagc gccgaagagc tgcggcagca aacgcaaagc gccacgctgg
     4081 aagaagcatt tataaatctg ttaccgcaag cgcaacgcca ggcgcatcag gcggtagtga
     4141 tcccaccgta taaacctgaa aatgcagaga ttgccatcga agcgcgcgat ctgaccatgc
     4201 gttttggttc cttcgttgcc gttgatcacg tcaacttccg cattccacgc ggggagattt
     4261 ttggttttct tggatcgaac ggctgcggta aatccaccac catgaaaatg ctcaccggac
     4321 tgctgcccgc cagcgaaggt gaggcgtggc tgtttgggca accggttgat ccaaaagata
     4381 tcgatacccg ccgtcgggtg ggctatatgt cgcaggcgtt ttcgctctat aacgaactca
     4441 ccgtgcggca aaaccttgag ttacatgccc gtttgtttca catcccggaa gcggaaattc
     4501 ccgcgcgagt ggctgaaatg agcgagcgtt ttaagctcaa cgacgttgaa gatgttctgc
     4561 cggagtcatt gccgctcggc attcgccagc ggctttcgct ggcggtggcg gtgattcatc
     4621 gcccggagat gttaatcctc gatgagccta cttctggtgt cgatccggtg gcgagggata
     4681 tgttctggca gttgatggtc gatctctcgc gccaggacaa agtgactatc ttcatctcca
     4741 cccactttat gaacgaagcg gaacgttgcg accgcatctc actgatgcac gccggaaaag
     4801 tgcttgccag cggtacaccg caggaactgg ttgagaaacg cggagccgcc agtctggaag
     4861 aggcatttat cgcctatttg caggaagcgg cagggcagag caacgaagcc gaagcgccgc
     4921 ccgtggtaca cgacaccacc cacgcgccgc gtcagggatt tagcctgcgc cgtctgttta
     4981 gctacagccg ccgcgaagcg ctggaactgc gacgcgatcc agtacgttcg acgctggcgc
     5041 tgatgggaac ggtgatcctg atgctgataa tgggttacgg catcagtatg gatgtggaaa
     5101 acctgcgctt tgcggtgctc gaccgcgacc agaccgtcag tagccaggcg tggacactca
     5161 acctctccgg ttcccgttac tttatcgaac agccgccgct caccagttat gacgagcttg
     5221 atcgtcggat gcgtgcgggc gatatcacgg tggcgattga gatcccgccc aatttcgggc
     5281 gcgatatcgc gcgtggtacg cctgtggaac tcggcgtctg gatcgacgga gcgatgccga
     5341 gccgtgctga aacggtaaaa ggttacgtgc aggccatgca ccagagctgg ttacaggatg
     5401 tggcgagccg acaatcgaca cccgccagcc aaagcgggct gatgaatatt gagacgcgct
     5461 atcgctataa cccggacgta aaaagcctgc cagcgattgt tccggcggtg atcccgcttc
     5521 tgctgatgat gatcccgtca atgctaagcg cccttagcgt ggtgcgggaa aaagagcttg
     5581 ggtcgattat caacctttac gtgaccccca ccacgcgtag tgaatttttg cttggtaaac
     5641 agttgccata catcgcgctg gggatgctga actttttcct gctctgcggc ctgtcggtgt
     5701 ttgtgtttgg cgtaccgcat aaaggcagtt tcctgacgct caccctggcg gcgctgctgt
     5761 atatcatcat tgccaccgga atggggctgc tgatctccac ctttatgaaa agccagattg
     5821 ccgccatttt cggaacggcg attatcacgt tgatcccggc gacacagttt tccgggatga
     5881 tcgatccggt agcttcgctg gaagggcctg gacgttggat cggcgaggtt tacccgacca
     5941 gtcattttct gactatcgcc cgcgggacgt tctcgaaagc gctggatctg actgatttgt
     6001 ggcaactttt tatcccgttg ctgatagcca tcccgctggt gatgggctta agtatcctgc
     6061 tgctgaaaaa acaggaggga tgatgcgcca tttacgcaat atttttaatc tgggtatcaa
     6121 agagttgcgc agtctgctcg gtgataaagc gatgctgacg ctgattgtct tctcgtttac
     6181 ggtgtcggtg tattcgtcag cgaccgttac gccaggatcg ttgaacctcg cgccgatcgc
     6241 cattgccgat atggatcaat cgcagttatc gaaccggatc gttaacagct tctatcgtcc
     6301 gtggtttttg ccaccggaga tgatcaccgc cgatgagatg gatgccggac tggacgccgg
     6361 acgctatacc ttcgcgataa atattccgcc taattttcag cgtgatgtcc tcgccggacg
     6421 ccagccggat attcaggtga acgtcgatgc cacgcgcatg agccaggcat ttaccggcaa
     6481 tgggtatatc cagaatatta tcaacggtga agtgaacagc tttgtcgcgc gctaccgtga
     6541 taacagcgaa ccgttggtat cgctggaaac ccggatgcgc tttaacccga acctcgatcc
     6601 cgcgtggttt ggcggggtga tggcgatcat caacaacatt accatgctgg cgattgtatt
     6661 gaccggatcg gcgctgatcc gcgagcgtga acacggcacg gtggaacact tactggtgat
     6721 gccgataacg ccgtttgaga tcatgatggc gaagatctgg tcgatggggc tggtggtgct
     6781 ggtggtatcg ggattatcgc tggtgctgat ggtgaaaggt gtactgggcg taccgattga
     6841 aggctcgatc ccgctgttta tgctgggcgt ggcgctcag
//
LOCUS       DAJOSY010000165         6681 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJOSY010000165 DAJOSY010000000
VERSION     DAJOSY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.165"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            62..838
                     /gene="exuR"
                     /locus_tag="OHN27_004070"
     CDS             62..838
                     /gene="exuR"
                     /locus_tag="OHN27_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCQ2288415.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADTNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1183..1845
                     /locus_tag="OHN27_004071"
     CDS             1183..1845
                     /locus_tag="OHN27_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2288416.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1849..2232
                     /gene="mzrA"
                     /locus_tag="OHN27_004072"
     CDS             1849..2232
                     /gene="mzrA"
                     /locus_tag="OHN27_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCQ2288417.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2379..2747
                     /locus_tag="OHN27_004073"
     CDS             2379..2747
                     /locus_tag="OHN27_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCQ2288418.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2785..3090
                     /locus_tag="OHN27_004074"
     CDS             2785..3090
                     /locus_tag="OHN27_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCQ2288419.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3093..3497
                     /locus_tag="OHN27_004075"
     CDS             3093..3497
                     /locus_tag="OHN27_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCQ2288420.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3487..3786
                     /locus_tag="OHN27_004076"
     CDS             3487..3786
                     /locus_tag="OHN27_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCQ2288421.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3972..4364
                     /locus_tag="OHN27_004077"
     CDS             3972..4364
                     /locus_tag="OHN27_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCQ2288422.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4434..5420
                     /gene="yqjG"
                     /locus_tag="OHN27_004078"
     CDS             4434..5420
                     /gene="yqjG"
                     /locus_tag="OHN27_004078"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCQ2288423.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5713..6078
                     /locus_tag="OHN27_004079"
     CDS             5713..6078
                     /locus_tag="OHN27_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCQ2288424.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6320..6676
                     /locus_tag="OHN27_004080"
     CDS             6320..6676
                     /locus_tag="OHN27_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCQ2288425.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
BASE COUNT         1672 a         1618 c         1645 g         1746 t
ORIGIN      
        1 ggcataccca taaataaagt ggtatgataa ctttgtccgc attccactcc gggagacgca
       61 tatggaaatc actgaaccac gccgtttgta tcaacaactt gccgctgacc tgaaagagcg
      121 catcgaacag ggcgtctatc tggtgggtga taaactgcct gccgagcgct ttattgccga
      181 tgaaaagaac gtcagccgta cggtagttcg tgaagccatc atcatgctgg aagttgaagg
      241 ctatgtcgaa gtgcgtaaag gttcaggtat tcatgtagtt tccaaccagc cgcgccatca
      301 gcaggcggct gacactaata tggaattcgc taattacggt ccgtttgagt tacttcaggc
      361 tcgccagctc atcgaaagta atattgccga gttcgcagca actcaggtaa cgaaacagga
      421 catcatgaaa ttgatggcca tccaggagca ggcgcgaggc gaacaatgct tccgtgattc
      481 cgagtgggat ttgcagttcc atattcaggt agccctggcg acgcagaact ccgccctggc
      541 ggctatcgtt gaaaaaatgt ggacccagcg tagccataac ccgtactgga aaaaactgca
      601 cgaacacatt gattcccgta ccgtcgataa ctggtgtgat gaccacgatc aaatcctcaa
      661 ggcgctgatt cgcaaagatc ctcatgctgc taagctggca atgtggcagc acctggaaaa
      721 caccaagatc atgttattca acgaaaccag cgacgacttc gagttcaatg ccgaccgcta
      781 tctattcgcc gaaaacccgg tggtgcatct cgatacagcc accagcggca gtaaatgaaa
      841 ttcctgtccg acaggcgctg caatgcgcct gtttgtctcg tcggtaagcg aaaagtataa
      901 agtgtcagcc tgtgtaaatc ctctcgccac cctcccctgc attcagcaaa atcagactcc
      961 acggacatgt aattttgata acgaacaacg ttgacctttg ttacaattag attcaatttg
     1021 aatttatgtt tttgaatgct ttcttatctc acgatttaac agggaatagt tcaggctgtg
     1081 ttgatgtatc aaacccgcag aacataccaa aacagcaata acattgcggt agtgcatctt
     1141 ttaaaaccag cgtggcgtta accgattcac caggaataat gaatggaact tttgacccaa
     1201 ttgctgcaag ccctgtgggc gcaggatttt gaaaccctgg ccaatccatc gatgattggc
     1261 atgttgtatt ttgtcttgtt tgtaattttg ttccttgaaa acggcttgct tccggcggcc
     1321 tttttaccgg gcgacagttt actggtattg gtcggcgtgt tgattgcgaa aggcgcgatg
     1381 ggctatccgc aaacgattct gctgctgacc gttgccgcca gcctcggctg ctgggtcagc
     1441 tatattcagg ggcgatggct gggcaatacc cgcaccgtac aaaactggct atctcattta
     1501 cccgcgcatt atcatcaacg cgcacaccat ctttttcata aacacggttt atcggcgctg
     1561 ttaattggtc gctttattgc gtttgtcaga acactgctgc cgacgattgc cgggttatca
     1621 gggctgaata acgcgcgctt tcagtttttc aactggatga gcggtctgct gtgggtattg
     1681 atcctgacaa ctctgggtta catgctcggc aaaacgccgg tatttttaaa gtacgaggac
     1741 cagctgatgt catgcctgat gctgctcccg gtggtgctgc tggtgtttgg cctggcaggt
     1801 tctctggtcg tgttatggaa aaagaaatat ggaaatcggg ggtaagggat gcaaatacct
     1861 cgcatgtcgc ttcgccagct agcctggtcc ggcgctgttt tacttctggt cggcacgctg
     1921 ttgctggcct ggtccgcggt tcgccagcaa gagtctacgc tggcgattcg tgccgttcat
     1981 caaggcacaa cgatgccaga cggtttttca atctggcatc accttgacgc tcatggcatt
     2041 cctttcaaaa gtatcacccc caaaaacgac actctgttaa ttacctttga ctccagcgac
     2101 cagagcgccg ctgcaaaagc ggtcctcgac agaacattgc cccaaggcta catcattgcg
     2161 cagcaggaca ataacagtca ggctatgcag tggctgaccc ggctacggga taactctcat
     2221 cgcttcggat aacttcctgt tatccgaaac agatcactca ctttggtgat ttcaccgtaa
     2281 ctgtctatga ttaatgaggc ggtggttaac actaccgctt cgttgtctgg acaatcgttc
     2341 ctttgtaata ggtccgaatc acaatggaag gttcaagaat gaaataccgc atcgctttag
     2401 ctgtttctct ttttgctctt agtgctggta gttatgccac taccctgtgt caggaaaagg
     2461 agcaaaatat ccttaaggag atcagctatg ccgaaaaaca ccaaaaccag aatcgtattg
     2521 acggtctgaa taaagccctg agtgaagtcc gggccaactg ttcagatagc cagctgcgtg
     2581 ccgatcatca gaagaaaatc gcaaagcaga aagatgaggt ggcggaacgc cagcaagatt
     2641 tagccgaggc gaagcaaaaa ggcgatgccg ataagattgc caaacgcgaa cggaaactgg
     2701 cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc acaatagtca
     2761 ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca tctgcgtgct
     2821 gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg cgagaagtcg
     2881 aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca gagccgttat
     2941 cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc ggcgcgtgcc
     3001 gatgagtatg tgcgtgaaaa tccatggacg ggcgtgggca ttggcgctgc aatcggtgta
     3061 gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac gcgcaagggc
     3121 ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt gaaatggtgg
     3181 aaacacgtct gcggctggcg gtggttgagc tggaagagga aaaagctaat ctctttcaac
     3241 ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg agcctgatgg
     3301 tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt gccaccaccg
     3361 tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa tcgcgtaaat
     3421 ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcagctg ctcgaggagg
     3481 agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct tagccagatc
     3541 cagcaacaac ggctggatct ttctgccagt cgtcgtgaat ggctggaggc aacaggcgct
     3601 tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt tggcagtagc
     3661 gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc cagacgcggt
     3721 tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca acagcttcgc
     3781 ggttaacttc cctctggccg gagccattcc ggccttaccc atcaaatttt ttgaagattt
     3841 ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc tccatcgaca
     3901 gcaactacgc taataccacg cccttgcaca aaaaaacaat cagcagcctg agtggcgcag
     3961 tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcacgc attttaatgc
     4021 cgattctgtt tattaccgct ggctggggaa aaattactgg ctacgcgggt acccaacaat
     4081 atatggaagc aatgggcgtc ccgggtttta tgctgccgct ggtgattctg cttgagttcg
     4141 gtggtggtct ggcaatcctg ttcggtttcc tgactcgcac cactgccctg tttactgcgg
     4201 gctttacgct gctgacggca tttttatttc acagcaactt tgctgaaggc gtcaactcgc
     4261 tgatgttcat gaaaaacctg acaatttctg gcggattcct gctgctggca attaccggtc
     4321 cgggcgcgta tagcatcgac cgcctgctga ataaaaagtg gtaagcacgc gctatactta
     4381 acgataaaaa acgaggagga agctcctcgt ttttgctatt ggaggagaga aaaatgggtc
     4441 aactgattga cggcgtctgg catgacacct ggtacgatac caaatctacc ggcggtaaat
     4501 ttcaacgttc agcttccgca tttcgtaact ggctcactgc cgatggcgct cctggcccca
     4561 ctggcacagg cggttttatc gcagagaaag atcgttatca tctctatgtt tcactcgcct
     4621 gcccgtgggc gcaccgcacg ctgatcatgc gcaaactcaa aggactggaa ccgtttattt
     4681 ccgtttccgt agtgaacccg ctgatgctgg aaaacggctg gacctttgat gacagttttc
     4741 cgggagcaac cggagacacg ctctatcaac atgaattttt gtatcagctt tatctccacg
     4801 ccgatcccca ctacagcgga cgagttactg ttcccgtgct gtgggacaaa aagaaccaca
     4861 ccatcgtcag caacgaatca gcagaaatca tccgcatgtt taataccgcg tttgatgcgc
     4921 tgggcgcgaa agcgggtgat tactacccac cagccctgca aacgaaaatt gacgaactta
     4981 acggctggat ttatgacacc gttaacaacg gcgtgtataa agctggtttt gccaccagcc
     5041 agcaagccta cgacgaagcg gtggagaaag tgtttgaatc gctggcgcga ctggaacaga
     5101 ttttaggtca gcatcgttac ctgaccggca accagctaac cgaagccgat attcgcctgt
     5161 ggaccacgct ggtccgtttt gatccagtgt atgtgaccca cttcaagtgt gataagcacc
     5221 gcatcagcga ttaccttaat ctgtatggct tcctgcgcga tatctaccag atgccgggaa
     5281 ttgccgaaac agtcaatttc gatcacatcc gtaatcatta cttccgcagc cataagacca
     5341 tcaaccctac ggggattatt tcaattggtc cgtggcagga tctcgatgaa ccgcatggac
     5401 gagatgttcg cttcggttaa aaaaaggcac cttacgaggt gcctctgata attagctaag
     5461 tgcatgttgt tcatgtcgga tgcggcgtga acgccttatc aaacctacaa aacatttata
     5521 ttcaatatgt tgcaatgacc gcgcaggcca gataagcgta gcgcatctgg cgattttgcg
     5581 cttgtcatca gtcttagcac gccttcatag ccctttatta attccttgaa cgaatattta
     5641 ctgccatttc attcacgtct attcttaatt tgctgcttta aaaacaagtg attgagcaaa
     5701 attgaggcaa aaatggactg gtatctgaaa gtactaaaaa attatgtcgg tttccggggt
     5761 cgtgcgcggc gcaaagagta ctggatgttt attctggtca acatcatctt tacgttcgtg
     5821 ctggggctgc tggataaaat gttaggctgg caacgcgctg gtggcgaagg catcctgacg
     5881 acaatctacg gtattctggt gtttttaccg tggtgggcag ttcagttccg ccgcctgcac
     5941 gacaccgacc gctcggcgtg gtgggcactg ctattcttaa tcccgtttat cggctggctt
     6001 attatcatcg tctttaactg ccaggcgggt acgccaggcg agaaccgctt tggaccagat
     6061 ccaaaactgg aaccttaaaa caaatctgct cgccggatgt ctgactcatc cggcttttat
     6121 catcatgtta atgaatgaat aatttactaa tgtttattta aaatatttca atgtcgttat
     6181 ataaattacc tataaaaaat aaccatgtat ttatcttaaa tatcaaaacc tttataacat
     6241 ccgaaaaaga aagatacgaa tagcatttac acaatacccg gaatagactt tcatcacttt
     6301 taattaaagg gatgttttta tgcagtggta tttgtccgta ttaaaaaatt atgttggttt
     6361 ctctggtcgc gcacgtcgta aagagtactg gatgtttact ctgattaacg ccatcgtcgg
     6421 cgctattatc aatgtcattc aattgatttt aggtctggag cttccatatc tgtctatgct
     6481 ttatttgctg gcaaccttcc ttcccgtcct tgcgcttgct atccgtcgct tacacgatac
     6541 cgaccgttca ggtgcctggg ccttgctctt ttttgttccg tttattggct ggctcgtgct
     6601 tctggttttt ttctgcacag aaggtacttc tggcagcaat cgttacggaa acgatccgaa
     6661 gtttggttca aattaatctt a
//
LOCUS       DAJOSY010000166         6565 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJOSY010000166 DAJOSY010000000
VERSION     DAJOSY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.166"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            142..1107
                     /gene="iaaA"
                     /locus_tag="OHN27_004081"
     CDS             142..1107
                     /gene="iaaA"
                     /locus_tag="OHN27_004081"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCQ2288426.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            1094..2965
                     /gene="gsiA"
                     /locus_tag="OHN27_004082"
     CDS             1094..2965
                     /gene="gsiA"
                     /locus_tag="OHN27_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCQ2288427.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQTLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            2985..4523
                     /gene="gsiB"
                     /locus_tag="OHN27_004083"
     CDS             2985..4523
                     /gene="gsiB"
                     /locus_tag="OHN27_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCQ2288428.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            4541..5461
                     /gene="gsiC"
                     /locus_tag="OHN27_004084"
     CDS             4541..5461
                     /gene="gsiC"
                     /locus_tag="OHN27_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCQ2288429.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVIARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            5464..6375
                     /gene="gsiD"
                     /locus_tag="OHN27_004085"
     CDS             5464..6375
                     /gene="gsiD"
                     /locus_tag="OHN27_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCQ2288430.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
BASE COUNT         1459 a         1578 c         1911 g         1617 t
ORIGIN      
        1 gtatgccacc tcgggcgtag cgctgggtcc tgcctttaca tgccatatcc atctttctat
       61 attcaaaaat tgaatgagta attcataaaa attctgatat ttatagcaaa agtggcgaac
      121 cacccttaat ggacgaatac tatgggcaaa gcagtcattg caattcatgg tggcgcaggt
      181 gcaattagcc gcgcgcagat gagtctgcaa caggaattac gctacatcga ggcgttgtct
      241 gccattgttg aaaccgggca gaaaatgctg gaagcgggcg aaagtgcgct ggatgtggtg
      301 acggaagcgg tgcgtctgct ggaagagtgt ccactgttta acgccggaat tggcgctgtc
      361 tttacgcgtg atgaaaccca tgaactggac gcctgtgtga tggatggtaa caccctgaaa
      421 gccggtgcgg tggcgggcgt tagtcatctg cgtaatccgg ttcttgccgc ccggctggtg
      481 atggagcaaa gcccgcatgt gatgatgatt ggcgaagggg cagaaaattt tgcgtttgct
      541 catggcatgg agcgcgtctc gccggagatt ttctccacgc ctttgcgtta tgaacaacta
      601 ctggcagcgc gcgaggaagg ggcaaccgtc ctcgaccata gcggtgcgcc actggatgaa
      661 aaacagaaaa tgggcaccgt gggggccgtg gcgttggatt tagacggcaa tctggcggca
      721 gccacgtcca cgggcggaat gaccaataaa ttacccggac gagttggcga tagtccctta
      781 gtgggggccg gatgctacgc caataacgcc agtgtggcgg tttcttgtac cggcacgggc
      841 gaagtcttca tccgcgcgct ggcggcatat gacatcgccg cgttaatgga ttacggcgga
      901 ttaagtctcg cggaagcctg cgagcgggta gtaatggaaa aactccctgc gcttggcggt
      961 agcggtggct taatcgctat cgaccatgaa gggaatgtcg cgctaccgtt taacaccgaa
     1021 ggaatgtatc gcgcctgggg ctacgcaggc gatacgccaa ccaccggtat ctaccgtgaa
     1081 aaaggggaca ccgttgccac acagtgatga acttgatgcc ggtaatgtgc tggcggttga
     1141 aaatcttaat attgccttta tgcaggacca gcagaaaata gctgcggtcc gcaatctctc
     1201 ttttagtctg caacgcggtg agacgctggc aattgttggc gaatccggct ccggtaagtc
     1261 agtgactgcg ctggcattga tgcgcttgtt ggaacaggcg ggcggtttag tgcagtgcga
     1321 tcaaacgctg ttgcggcggc gcagtcgtga agtgattgaa cttagcgagc agagcgctgc
     1381 acaaatgcgt catgtgcgcg gtgcggatat ggcgatgata tttcaggagc cgatgacatc
     1441 gctgaacccg gtatttactg tgggtgaaca gattgccgaa tcaattcgtc tgcatcaaaa
     1501 cgccagtcgt gaagaagcga tggtcgaggc gaagcggatg ctggatcagg tacgcattcc
     1561 ggaggcacaa accattcttt cacgttatcc gcatcaactc tctggcggga tgcgccagcg
     1621 agtgatgatt gcgatggcgc tgtcatgccg cccggcggtg ctgattgccg atgagccaac
     1681 caccgcgctg gatgtcacta ttcaggcgca gatcctgcaa ttaatcaaag tattgcaaaa
     1741 agagatgtcg atgggcgtta tctttatcac tcacgatatg ggcgtggtgg cagagattgc
     1801 cgatcgggta ctggtgatgt atcagggcga ggcggtggaa acgggtagcg tcgaacagat
     1861 ttttcatgca ccgcaacatc cttacacccg tgcgctgtta gctgctgttc cgcaacttgg
     1921 tgcgatgaaa gggttagatt atccccgacg tttcccattg atatcgctag aacatccagc
     1981 gaaacaggcc ccccccatcg agcagaaaac ggtggtggat ggcgaacctg ttttacgggt
     2041 gcgtaatctg gtcacccgtt tccctttgcg cagcggtttg ttgaatcgcg taacgcggga
     2101 agtgcatgcc gttgagaaag tcagttttga tctctggcct ggcgaaacgc tatcgctggt
     2161 gggtgagtct ggcagcggta aatccactac cgggcgggcg ttgctgcgcc tggtcgaatc
     2221 gcagggcggc gaaattatct ttaacggtca gcgaatcgat accttgtcac ccggcaaact
     2281 tcaggcattg cgccgggata ttcagtttat ttttcaggac ccttacgctt cgctggaccc
     2341 acgtcagacc atcggtgatt cgattatcga accgctgcgt gtacacggtt tattgccagg
     2401 taaagacgcg gctgcacgcg ttgcgtggtt gctggagcgc gtgggcctgt tacctgaaca
     2461 tgcctggcgt tacccgcatg agttttccgg cggtcagcgc cagcgcatct gcattgctcg
     2521 cgcgttggca ttgaatccga aagtgatcat tgccgacgaa gccgtctcgg cgctggatgt
     2581 ttctattcgc gggcagatta tcaacttgtt gctcgatctc cagcgtgatt tcggcattgc
     2641 gtatctgttt atctcccacg atatggccgt ggtagagcgg attagtcatc gtgtggcggt
     2701 gatgtatctc ggacaaattg ttgaaattgg tccacggcgc gcggtcttcg aaaacccgca
     2761 gcatccttat acgcgtaaat tactggcggc agttccggtc gctgaaccgt cccgacaacg
     2821 accgcagcgt gtactgctgt cggacgatct tcccagcaat attcatctgc gtggcgaaga
     2881 ggtggcagcc gtctcgttgc aatgcgtcgg gccggggcat tacgtcgcac aaccacaatc
     2941 agaatacgca ttcatgcgta gataacattc aggcggagaa taaaatggca agagctgtac
     3001 accgtagtgg gttagtggcg ttgggcattg cgacagcgtt gatggcatct tgtgcattcg
     3061 ctgccaaaga tgtggtggtg gcggtaggat cgaacttcac cacgctcgat ccgtatgacg
     3121 caaatgacac gttatctcag gccgtagcga aatcgtttta ccaggggctg tttggtctgg
     3181 ataaagagat gaaactgaaa aacgtgctgg cggagagtta taccgtttcc gatgacggcc
     3241 ttacatacac cgtgaaattg cgggaaggaa ttaaattcca ggatggcacc gatttcaacg
     3301 cagcggcggt aaaagcgaat ctggaccggg ccagcgatcc ggcgaatcat cttaaacgct
     3361 ataacctgta taagaatatt gccaaaacgg aggcgatcga tccgacaacg gtaaagatta
     3421 ctctcaaaca gccgttctca gcgtttatta atattcttgc ccatccggcg accgcgatga
     3481 tttcaccggc agcgctggaa aaatatggca aggagattgg ttttcatccg gtgggaaccg
     3541 gaccgtatga actggatacc tggaatcaga ccgattttgt gaaggtgaaa aaattcgcgg
     3601 gttactggca gccaggattg cccaaactgg acagcataac ctggcgtccg gtggcggata
     3661 acaacacccg cgcggcaatg ctgcaaaccg gtgaagcgca gtttgctttc cccattcctt
     3721 acgagcaggc cgcactgctg gagaaaaaca aaaatatcga gttgatggcc agtccgtcaa
     3781 ttatgcagcg ttatatcagt atgaacgtga cgcaaaaacc gttcgataac ccgaaggtcc
     3841 gtgaggcgct gaattatgcc attaaccgcc cggcgctggt gaaagtagcc ttcgcgggct
     3901 atgcaacgcc agctactggt gtggtaccgc cgagtatcgc ctacgcgcaa agttataaac
     3961 cgtggcctta cgatccagtg aaagcgcgcg aattactgaa agaggcggga tatcccaacg
     4021 gtttcagtac cacgctgtgg tcgtcacata accacagcac cgcgcagaaa gtgctgcaat
     4081 ttacccagca gcagttagcg caggtcggga ttaaagccca ggtgactgcg atggatgccg
     4141 gacagcgggc ggcagaagtt gaaggtaaag ggcaaaaaga gagcggcgtg cggatgttct
     4201 acactggctg gtcggcttca accggcgaag ctgactgggc actatcgccg ctgtttgcct
     4261 cgcaaaactg gccaccgacg ctgtttaata ccgcgtttta cagcaataaa caggtggatg
     4321 acttcctggc tcaggcactg aaaactaatg atccggcgga aaagacccgc ttatataagg
     4381 cggcgcagga tatcatctgg caagagtcgc cgtggatccc gctggtggta gaaaaactgg
     4441 tgtcggcaca cagtaaaaac ctgaccggtt tttggatcat gccagacacc ggcttcagct
     4501 ttgaagacgc ggatttgcaa taagcaacgc agggaatgga atgcttaatt acgttatcaa
     4561 acgattactg gggttgattc cgacgctgtt tatcgtctcg gtgctggtgt ttttatttgt
     4621 ccatatgctg cccggcgatc cggcgcgatt gattgccggg cccgaagctg atgcgcaggt
     4681 tatagaactg gtgcgtcagc agctggggtt ggatcagccg ctgtatcacc agttctggca
     4741 ctatatcagc aatgctgtgc agggggattt tggcctgtcc atggtgtcgc gtcgtccggt
     4801 tgccgatgag attgccagcc gctttatgcc aacgctgtgg ctgaccataa ccagtatggt
     4861 ctgggcggtg atatttggta tggcggcggg aattatcgcc gccgtctggc gtaaccgttg
     4921 gccggatcga ttgagtatga ccattgcggt gtcggggatc tcgtttccgg catttgcgct
     4981 ggggatgctt ttaattcagg tattctccgt tgaactgggc tggctgccta ccgtgggagc
     5041 agacagttgg cagcactaca ttttaccctc cctgacgctc ggcgcggcag tggccgccgt
     5101 gatagcgcgc tttacccgcg cgtcgtttgt cgacgtttta agcgaagatt atatgcgtac
     5161 cgcgagggcg aaaggggtga gcgaaacctg ggttgtcctc aaacacgggc tacgcaacgc
     5221 gatgatcccg gtagtgacca tgatgggctt acagtttggc tttttgctcg gtggttccat
     5281 cgtggtggag aaagttttca actggcccgg acttggacgc ttactcgttg actccgtaga
     5341 aatgcgcgat tacccagtga ttcaggcgga aattctgctt ttctcgctgg aatttattct
     5401 tatcaactta gtggtggatg tgctttacgc cgccattaac ccggctatca ggtacaagta
     5461 aggatgcgac tatttaactg gcgacgtcag gcggtgttaa acgccatgcc actggtcaaa
     5521 cctgaccagg tgcgtacacc gtggcatgaa ttctggcgac gatttcgccg tcagcatatg
     5581 gcgatgaccg ccgcattatt cgttatttta ttgattgtgg tggccatttt tgcacgctgg
     5641 atcgctccct atgacgccga aaattatttt gattatgaca atctgaataa cggaccttct
     5701 ttgcagcact ggtttggcgt cgattcactg gggcgcgaca ttttcagccg tgtcctggtt
     5761 ggtgcgcaaa tctcgctggc ggcgggcgtg tttgccgtgt ttatcggtgc ggcgataggg
     5821 acgttgctgg gcttgttggc tggatattat gaaggctggt gggatcggct gatcatgcgc
     5881 atttgcgatg tgctgtttgc cttcccgggt attttactgg cgatcgctgt tgttgcggtg
     5941 ttgggaagcg gcattgctaa cgtgattatc gcagtcgcca ttttttccat ccccgcgttt
     6001 gcccgcctgg tgcgcggcaa cacgctggtg ttgaaacagc aaacctttat tgagtcagca
     6061 cgcagtattg gtgccagcga tatgaccatt ttattgcgtc atatcctgcc tgggaccgtc
     6121 tcttctatcg tggtgttttt caccatgcgc attggcacct cgattatctc tgccgccagc
     6181 ctgtcatttc tcggcctcgg tgcgcagccg ccgacaccag agtggggagc aatgctcaat
     6241 gaggcacgag cggatatggt tatcgcgccg catgtcgctg tttttccggc cctggctatt
     6301 tttctgaccg tactggcgtt caatttgttg ggcgatggtt tacgcgatgc gctggatccg
     6361 aaaattaaag gatagttacg tttgaatatt gcttgaaagg gtaatcacct cacaggaaat
     6421 tattgcccta agcaagtgtt gtaactttct gctgattttg tagaatcggg taatttggtt
     6481 aaaaagccgc agcaagggac tatttttgca gcggcacagc gttcagatag ttattttgtt
     6541 aaatgtatta acatgctgag tttat
//
LOCUS       DAJOSY010000167         6376 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJOSY010000167 DAJOSY010000000
VERSION     DAJOSY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.167"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..356
                     /locus_tag="OHN27_004086"
     CDS             <1..356
                     /locus_tag="OHN27_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288431.1"
                     /translation="VGSIFAAIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLE
                     DIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHL
                     KSKATAPIQNGPLPA"
     gene            complement(360..1751)
                     /gene="tcyP"
                     /locus_tag="OHN27_004087"
     CDS             complement(360..1751)
                     /gene="tcyP"
                     /locus_tag="OHN27_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCQ2288432.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1884..2474)
                     /locus_tag="OHN27_004088"
     CDS             complement(1884..2474)
                     /locus_tag="OHN27_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCQ2288433.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2637..3305)
                     /gene="hxpB"
                     /locus_tag="OHN27_004089"
     CDS             complement(2637..3305)
                     /gene="hxpB"
                     /locus_tag="OHN27_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCQ2288434.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLADVKLSSLTELTAK
                     DLLG"
     gene            3452..3988
                     /locus_tag="OHN27_004090"
     CDS             3452..3988
                     /locus_tag="OHN27_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCQ2288435.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4029..4889)
                     /locus_tag="OHN27_004091"
     CDS             complement(4029..4889)
                     /locus_tag="OHN27_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCQ2288436.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4995..5285)
                     /gene="ghoS"
                     /locus_tag="OHN27_004092"
     CDS             complement(4995..5285)
                     /gene="ghoS"
                     /locus_tag="OHN27_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCQ2288437.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5386..6315)
                     /gene="pfkB"
                     /locus_tag="OHN27_004093"
     CDS             complement(5386..6315)
                     /gene="pfkB"
                     /locus_tag="OHN27_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCQ2288438.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1648 a         1594 c         1563 g         1571 t
ORIGIN      
        1 ctgtcgggag catttttgct gcgattcaaa aaaatgagag tctcctaaag tttccttcga
       61 taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg ggaatgggaa
      121 gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa cgtctcgggt
      181 atgaggtttt aagcttctcg ctacaggcac attcattgag ccaggaatgt attgataagt
      241 tggacatttt tttcgctgac gatctgttta aatatgagtc agtatgcatc gccgcgatgg
      301 aacatttaaa aagtaaggca acggccccta tacaaaacgg accgttgcca gcataagaat
      361 taatggtgtg ccagttcggc gtcgtcttcg ctatccagaa tggctttatc ggtttgcttc
      421 agccactggc tggtcagcgt gccagctgtc atcgagccac taacgtttaa cgccgtacgg
      481 cccatgtcga taagcggttc aacggagatt aacagcgcca ccagggttac tggcaggccc
      541 atcgcaggca gtacaatcag tgcggcgaaa gttgcaccac caccgacacc ggcaacgcct
      601 gcggaactaa cggtaacaat accgaccagc gtcgcaatcc acatcgggtc cagcgggtta
      661 atgccaaccg taggcgcaac catcaccgcc agcattgccg gatacaaacc ggcgcagccg
      721 ttctgaccaa tggttgcacc gaaagaggcg gcgaaactgg cgatggattc aggaacgccc
      781 agacgacgcg tttgtgcttc cacattcagt gggatagacg cagcactgga acggctggta
      841 aaggcaaacg tcagcacagg ccatacctta cggaagtact tcagcggact cacgccatta
      901 atgcccagca gaatgccatg cactgcaaac ataatcagca gaccgaggta ggacgcgaca
      961 acgaaacttc ccagtttgat gatgtcttgc aagttagaac ctgcaaccac tttggtcatt
     1021 agagccagaa cgccgtaagg ggtcaactgc atgaccaggc gaaccagttt catcacccag
     1081 ctttgtaggg tatcgatagc ggctaagacg cgttcacctt tcggcgcatc atccttcagc
     1141 agtttcaatg cagctacgcc gaggaatgcg gcaaaaatta ccacgctgat aattgacgtc
     1201 ggattggctc cggtaagatc ggcaaacggg tttttcggga taaaggacaa gaccagctgc
     1261 ggaacgctca gatcagagac tttaccaaca tagttacttt caatggcgtt cagacgtgca
     1321 gtttctgcac caccctgaac cagaccttca gccgtcaaac caaacaggtt ggtgaccagc
     1381 acaccgacca gcgccgcaat cagcgtggta aacaaaagcg taccgatggt cagaaaactg
     1441 attttgccta actgagatgc gttatgcaga cgggcaaccg cgctcagaat agaggcgaag
     1501 actaacggca taacgatcat ttgcagcagt tgaacatagc cgttaccaac gatgttaaac
     1561 cactgtacag aatctttaag tacctggctg tcagaaccat aaatggtatg cagggcaagg
     1621 ccaaaaacca cacccatcac cagacccacc agcacttttt tcgccagact ccactgttta
     1681 tggcgggtct gagccagcgc aaacagcagt acaacgaaca ccacgatgtt cgcaattaat
     1741 ggaaagttca tccccgttct cctgatctta ttatttctat cactgcgtag cagtcatagt
     1801 gttggcgcaa gattaacaga actgtgagct ggctcttata ttcaaatgga attgcttatt
     1861 ccagaaaggt gatttttgcc tttttagtat tcaacctgat gcttaataag ccgatgaaac
     1921 tggatctgca aggtattgat catttgcgat gaccaacgaa cagcgctgtt ctcgggtaat
     1981 gaatggggca tccataccga ccagacaaat aatgccatgc agataaaacg ttccagttgg
     2041 ttgccctttt ggggaaccag gataggcaag cggaaacgcc agcggcatgg ccagagcagg
     2101 ggaacaccgg cgggtgtcag catatcggca agtatgtggc tcaaataacc cagcaccatt
     2161 ccttgtagcg catcagccgg aatgaaccag ccttccggaa ccttaaggta aaaggttgcc
     2221 agcagcgcaa ataccgccag cagactgtgg gtaaaaccac gatgcccaaa agcgcgggcg
     2281 atcggttttg atatccattt taatcgctgc ccaagaaacg actttgggtg atcgatgtcc
     2341 ggtaacaaac acgtcaggat tgcggaaggg acaatatgcc accagtcacc ctgtgccagc
     2401 acgggcgtca gctcggcatt tttggcaaat accgcacaag caatagaaaa gagaaggtga
     2461 ccttccgccg tcatgataaa actcactaaa ctgtcgatac gtacagtata ggatttttat
     2521 acagtgcacg gaagtggtga cggtgtaact ctttctctta aattgacgat taatccgtca
     2581 gttgaatgca atttcagata aagaaccagg ggcagcgaga tcactgcccc tggtcattaa
     2641 ccgagaaggt cttttgcggt gagttctgtc agcgatgaga gtttgacgtc tgctaataca
     2701 aaacgtggat cattttgcgc ttctggcgca ggaacgacga tggaacgcat gcgggctgct
     2761 ttagaggcga tcatgccatt taccgaatct tccagcgcta cgcaggtcag agggtcaacg
     2821 cccagttttg ctgcgcagtc gagatatact tgcggatgcg gcttgctgta aggcagtttt
     2881 tcggccgagg cgagggcatc gaaactgtcg cgtaagtcaa acatggtcaa cactttttcc
     2941 agcatatgta gtggtgacgc ggaggccagt cccaccaata aaccttgttc tttgcataac
     3001 gcaacggctt cgcgcacgcc tggtaataat ggacgtgtct cttcaaccag tgaaatggca
     3061 cgggcaataa cccgttctac tacttcctga cggcttggcc cattccacgg ttgccgggcg
     3121 taccaaagat cgaccaccat atcgatgcgt aaacctaagg tgtccggcag ctcgttacga
     3181 cgggagatat ccacccccag gcttgccatc acatccagtt cggctcgatc ccataaaggt
     3241 tctgagtcga taagtaatcc atccatatca aaaattgcag caagaatctg acgcggggtt
     3301 gacattacaa cctctccttt agagcattat gtgtaaggta ttaagccgtg cttatgatta
     3361 agcgcctatc atatcgcctt taaagaatgg gcgaaaacag atatctacag gtaaactaag
     3421 tgaaacgacg cgtcttaatc aaggagaact catgacgtat caacaagctg gacgcattgc
     3481 tgttttgaaa aggattttgg gttgggtgat ttttatacct gcattgatct ctacattgat
     3541 ttctttgctg aaatttatga atacccggca ggaaaaccag gaaggcatta atgcggtcat
     3601 gctcgacttt actcatgtca tgatcgatat gatgcaggcg aatacgcctt ttttgaatct
     3661 attctggtat aactccccga cgcccaattt taatggtggc gtgaacgtta tgttctgggt
     3721 gattttcatc ctgatttttg tcggactggc gttgcaggat tccggtgccc ggatgagtcg
     3781 ccaggcgcgc ttcttgcggg aaggtgttga agatcaactc attctggaaa aagccaaagg
     3841 ggaagagggg ctaacgcgtg aacaaattga gtcccgtatt gttgttccgc accatactat
     3901 tttcctgcag tttttctcac tgtatatcct gccggttatt tgtattgctg caggttatgt
     3961 gttcttttct ctgcttgggt ttatttaatc aaaaggccgc acggagcggc cattatcctc
     4021 aacccatatc atgctgctaa taatctatcc aatgactgct gagcaataac caaatgctga
     4081 ccgccaaata accttgcacg atttaacagc gtgtagagtt ggtaaaccgg ttgacgttca
     4141 aggaaatcgg caggtagcgg ggatactgac tgatagccgt catagatttg tggcggctgt
     4201 tcagtatgca gcggtaacat cgccaggtcg cactctcggt caccccagta gcaggccggg
     4261 tcgaaaatgt acgggccatc cggacccagt gcacagttgc cggaccataa atcgccgtgc
     4321 aacagagaag gctgcggttg atgtgaggcc agacgctgct gaatatgctc gacgatggcg
     4381 tcgatattgc cgaaagcgat ccctttttct gcagccagtt ccaactgcca gccaatccgt
     4441 tgttcagcaa aaaacgttga ccagcgacgt tgccaggtgt tgggctgtgg agttgtggag
     4501 agcgcgttat cgaaatcaag gccaaattgt ggttggtcgc tccattgatg taaacgcgca
     4561 atttgctgac caagaataaa tgcgctatgc gcatccagcg gacgaggtgg gagataatcc
     4621 atcaccagaa aactgtagtc acggtcagcg ccaactgccc aaaccttagg cacggtgacg
     4681 gttttactac gcgacagtaa ctccagttgg tcggcttcgg cggtaaaacc gggaagcagt
     4741 tccctttcat cacatttgac gaaaaaatca tgtcctgcat agcgcaaatg ccatgcggca
     4801 tggacttctc cgccaggcag ttcattacgc agttcgattt cgccttcacc taactgctcg
     4861 ctcaaaagac gactgattgc ctgccacatg atgtctctcc catgttgtct gccagatcat
     4921 aaggttagcg caagaatgcg gtgtaaaaac acgagctaac gcacaccaca gactgattat
     4981 tgcccaaaaa ccatttattt tctgttgcta ttccattcct cccaggtcgt gatggtgatt
     5041 tcaggatctt tgccggttgc actttgggta agccccgaaa ccagttctct aatttcttct
     5101 tcactttggg tactgataag tccaaaagtg ttagttccca gctcatggat atttccctca
     5161 tcatccgtca tagtgagcaa aaaaccgtcg cgagtcaggt aattattcaa ctcgttaatt
     5221 tcagtcaatg tatcctcatg caacatgacg gttatgacat aacggacaag atcgccactg
     5281 gccatagttc acccctttgt tatcacatga cttttgagca tagtcggaga aacgcgttgc
     5341 cgacaggttg gtgatgattc ccccaatgct gggggaatgt ttttgttagc gggaaaggta
     5401 agcgtaaatt ttttgcgtat cgtcatggga gcacagacgt gttccctgat tgagtgtggc
     5461 tgcactcccc gcagctacgc caaaacgaac catctcttca agagaggcat tttctgccag
     5521 tttcagtgtc atcgcgccga ccatgctgtc accagcgcca acggtactct gacttttcac
     5581 cggtggtggc accacctgaa tacagttttc actatcaaca cccagcgctc cttgtggacc
     5641 cagggaaacg acaacccgtt tggccttgcc gctattaacg atttcctgcg cggctttgcg
     5701 gacatcgtcc ggctgggtga gttcgcgatt caccagcgca ctgagttctt tttggttagg
     5761 cttaaccaac tcgatgttac caattgccag tgctgcactt aacgcttcgc cagaactgtc
     5821 gacgatgcag cggatccctt gtttttgcgc agcggaaatc agttgggtta atttttccag
     5881 cttcacacct ggcggcaggc ttccgcttat gaccaggatg gccccggatt caatttccag
     5941 aacttgctct tcaagctggc gaaactcatc ttcatttaat gccgcgcctg gcataacaaa
     6001 acgatactgc tcaccgcttg cttccacatg tacgtgtaaa ttctgccggg tccagtcttt
     6061 ggcttctaca gtagcgacgg ggacattttc atccgccaac agtgaaacca ggtgttcgcc
     6121 ggtcgcgcca cccgccggga agatcgctgt ggcactgcct ccaagatggg caatggcgcg
     6181 ggcgacgttg atgccgccgc ccccgggttc gaacaccggt gcggtacagc gcagttttcc
     6241 ttcgggataa atttgcgggg taattgttgc gctatcgaga gagggcgcaa gtgtcaacgt
     6301 atagatacgt accatcattt cctcctatag gctgatttca gtctggcacc gaatcagcgg
     6361 aaagtgaaga aattaa
//
LOCUS       DAJOSY010000168         6271 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJOSY010000168 DAJOSY010000000
VERSION     DAJOSY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.168"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            127..477
                     /locus_tag="OHN27_004094"
     CDS             127..477
                     /locus_tag="OHN27_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCQ2288439.1"
                     /translation="MLILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            530..925
                     /locus_tag="OHN27_004095"
     CDS             530..925
                     /locus_tag="OHN27_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCQ2288440.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            966..1709
                     /gene="cmoA"
                     /locus_tag="OHN27_004096"
     CDS             966..1709
                     /gene="cmoA"
                     /locus_tag="OHN27_004096"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCQ2288441.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1706..2677
                     /gene="cmoB"
                     /locus_tag="OHN27_004097"
     CDS             1706..2677
                     /gene="cmoB"
                     /locus_tag="OHN27_004097"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCQ2288442.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2842..5271)
                     /gene="torZ"
                     /locus_tag="OHN27_004098"
     CDS             complement(2842..5271)
                     /gene="torZ"
                     /locus_tag="OHN27_004098"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCQ2288443.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTTKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(5296..>6271)
                     /locus_tag="OHN27_004099"
     CDS             complement(5296..>6271)
                     /locus_tag="OHN27_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCQ2288444.1"
                     /translation="HSMSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLK
                     ASKDIYHEFVSGKIDSDDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQS
                     ESAQKMHNKAQKGGETCIDCHKGIAHFPPEIKMDDNAAHELESQTATSVTNGAHIYPF
                     KTSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSE
                     EGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHA
                     PIAADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1419 a         1637 c         1508 g         1707 t
ORIGIN      
        1 tgcttgcata gtagaatcta accatccatc actcatagct aaccctcgga aatcttatct
       61 ttctgtctat cataaccaca agcatcacct ttaggaacca tctgaaacgg gaggtgtatt
      121 tagaccttgc taatcttaga cagccgcccg gttcatgcag cacgtccaca cagtgaagct
      181 attcgcgacg ctcaacgaaa aaaacctaaa gttccggtac atgctgtact gacggcgaca
      241 aatccgctga tccgttttat cggtagtgat gatatgacgc aaaaccggga attatttcag
      301 gtctggttac aaaaattagc gcagtggcat cagaccacta cgccttatct ttttttacat
      361 acgccagaca ttgcccaggc accggaactg gtacataccc tgtgggaaga cttacgtaaa
      421 acgcttccag agatcggagc agttccggct attccacagc aatcttctct tttctgaatt
      481 tgccacctat catagacagg tgccatcggc cattttaaag ggagtttgta tggtaagcgc
      541 gctgtatgcc gttttaagtg cgttgttatt aatgaagttc tcttttgatg tcgttcgcct
      601 gcgaatgcag taccgcgttg cctatggcga cggcggtttt agcgaactgc aaagcgctat
      661 tcgcattcat ggtaacgcgg tggaatatat tcccatcgcg attgtgctga tgctgtttat
      721 ggaaatgaat ggcgcagaaa cctggatggt gcatatttgc ggcatcgttt tgcttgctgg
      781 tcgtctgatg cattattacg gttttcatca ccgtctgttc cgctggcgac gttccggcat
      841 gagcgccacc tggtgtgcgc tgttgctgat ggtgctggcg aatctttggt atatgccctg
      901 ggagttggtt ttctccctgc gttagcgcac aatacgccac tttctttttc ccggattttt
      961 acgttatgtc tcaccgcgac acgctatttt ctgcccctat cgccagactg ggcgactgga
     1021 cctttgatga acgggtagct gaagtcttcc cggatatgat ccagcgttcc gtacccggct
     1081 attccaatat tatttccatg attggtatgt tagccgagcg cttcgttcaa cctggtacgc
     1141 aggtttacga tctgggttgt tctctgggcg cggcgacgct ctcggtgcgt cgcaacattc
     1201 atcatgataa ttgcaaaatt attgccatcg acaactcccc ggcgatgatt gaacgctgcc
     1261 gtcgtcatat tgacgcctat aaagccccta cgccagtaga cgtcattgaa ggtgatattc
     1321 gcgatatcgc cattgaaaac gcatcgatgg tggtgctgaa ttttaccctg caattcctgg
     1381 aaccttccga gcgccaggca ttactggata aaatttatca aggactgaac ccgggcggcg
     1441 cgctggtgct ttcggaaaaa ttcagtttcg aagatgccaa agttggtgaa ctgttgttca
     1501 acatgcacca cgactttaaa cgtgccaacg gttacagcga actggagatc agccagaagc
     1561 gcagcatgct ggaaaacgtg atgctgaccg attccgtgga aacccataaa gcacgcctgc
     1621 ataaagccgg ttttgagcat agtgagctgt ggttccagtg ctttaacttt ggttcactgg
     1681 tggcattaaa agcagaggac gctgcatgat cgactttggt aacttttatt ctctgattgc
     1741 caaaaatcat ctttcacact ggctcgaaac gctgcccgcg cagattgcta actggcagcg
     1801 cgagcagcag cacgggttgt ttaagcagtg gtcaaatgcg gtggagtttc tacctgaaat
     1861 taaaccgtat cgtcttgatt tattgcatag cgttacggca gaaagcgaag agccactgag
     1921 cgcagggcaa attaaacgca ttgaaacgct gatgcgcaac ctgatgccgt ggcgcaaagg
     1981 gccgttctca ctgtatggcg tcaacatcga taccgaatgg cgttccgact ggaaatggga
     2041 tcgtgttctg ccccatcttt ctgatttaac cggacgcacc attctggatg tcggctgcgg
     2101 cagcggttat cacatgtggc gcatgattgg cgcaggcgcg cacctcgcgg tggggatcga
     2161 ccctacgcaa ctttttctgt gccagtttga agcggtgcgt aaactactgg gtaatgatca
     2221 gcgcgcgcat ttgttaccgt taggtattga acaacttccg gcactgaaag cctttgatac
     2281 cgtcttttcg atgggcgtgc tttaccatcg ccgttcaccg ctggagcatc tctggcagtt
     2341 gaaagatcaa ctggtcaatg aaggtgaact ggtgctggaa acgctggtta ttgatggtga
     2401 tgagaacacg gtgctggtac caggcgatcg ttacgcgcaa atgcgtaatg tctatttcat
     2461 cccttccgcg ctggcgctga aaaactggct gaagaagtgt ggttttgtag atatccgcat
     2521 tgcagatgtg agcgttacca ctacagaaga gcaacgacgc accgaatgga tggttactga
     2581 atcgctggcc gatttcctcg acccacatga tccgagtaag acagttgaag gttatccggc
     2641 acctaaacgt gcggtgctga ttgcgcgcaa gccgtaaagg tctggtatta ctgccggatg
     2701 cggcgtgaac gccttatccg gcctacaaaa ccttgctaat tcaatatatt gcagggacta
     2761 tgtaggcctg ataagcatag cgcatcaggc agctttacgt ttgcataacc tcagcgcccg
     2821 tttccgggcg ctattcactt cttatgcctg aatagctggc tgatcaaacg ccgttaactt
     2881 cggcgcattg cccgtatatt tttcgatata caccagcgca gagttaccgg cacaggcatt
     2941 tgccagctgc gagctgggga tatccgccgt taacacgttc gcactgccgt ttttacacaa
     3001 gccattttcc agatctggcc atgcaccttc atgcacgcat accacgcctt ttttgatccc
     3061 gtcagtcacc accgcgcctg tcagaatctg accacgtttg ttccacacac gcaccagatc
     3121 accattcgca ataccaaagc gagcagcatc ttcggtgtga atagtgattg gttcacgatc
     3181 tgcaaccgca tattttttac gcagttccgc atagttaagc tgactatgta aacggtgtgc
     3241 cggatgtgcg gtcagaagct gcaactgctt ctcgtcggcg gtacccttcc actcatcagg
     3301 cgcaagccag gttgggtgtg ccgggcaatc cttatagcca aatttttcca gcgttttgga
     3361 gtaaatctca attttgccgc ttggcgtacc cagcgcattt ttcaccggat cggcgcggaa
     3421 atcaccataa cgaacgtact gttcgttctt ctcgctgcgg cgcatttcga tcagtttatt
     3481 ttgctgccag aaggcattaa acatcggcat agtcacgcgt tgcgcacggg cacctttctg
     3541 agcggcatca tagaaaaatt tcagccacgc catttcatct ttaccttcgg tatagatctc
     3601 ttttccgcca ggtttcagta attccgcaag atcggcaaac acatcaaaat cgtttcgcgc
     3661 ttcaaattgc ggagcgacag cctgcttcat cggcacaata tgctggttgc tgtaatcacc
     3721 ggtcatcgtc aagtcattgc gctcaaacga tgtggtgatc ggtaatacga tatctgcatg
     3781 tttagcagct gcggtccagt agcattcaga aacgacgatc atctccggtt tctgccatgc
     3841 tttaatcaga cgattagtat cctggtgatg ggtaaagtta ccgccacccg cccaccagat
     3901 cattttgata ttcggatacg tttgctcttt accgttatgc tgatatttgc cgccaggatt
     3961 ttccagcgca tcgacgatac gggcaacagg aatcgccgtc attccgccat catcagcggc
     4021 ctcgctggcc tgtccggcaa tcgccgcaga catttccggc agcacgccgc caacacgtgt
     4081 cgggttgcca ccattggagt aatgataaga gaaaccaaaa ccgccgcccg gtgtaccaat
     4141 ctgccctaac atcgccgcca gcgtcaccag catccagtgt ttctgttcac catattgctg
     4201 gcgctgaatt ccccagcctg ccatcagcat tgtgcgattc gccgccatca gttcagcgag
     4261 tttgactatc tgcgcttccg gtacgccagt aatttccgcc gcccaggccg cgctcttcgt
     4321 agtattatcg ctcttacctg tcagatattc ttcaaactgc ggatacccgg tggtgtattt
     4381 ttcaaggaac actttatcgt gtttgccttg tgtcatcagg gtatgcgcaa tccctaacat
     4441 cagtgccacg tcggtgccca tattcggcgc gatccaggtg gcattatcgc caaagaactc
     4501 aatggtttcg gagcggatag gatcaatggc aatcactggt ttgccagatt ttttcagctg
     4561 atggaagtat tccagccctt gctcatcggt actgctccag gcaattttta aggtattcag
     4621 cgggttcatc ccccacagca ccacaacctg gctgttttcc agaatcagcg gccaggaggt
     4681 ctgctgttca tacacctcta cagaaccgac cacatgcggc atgatcacct gtgctgcccc
     4741 ggttgaataa tcgccgctat gcccggaata accgcccgcg aggttcatat aacgttgcag
     4801 taaggtttgc gctttatgca acacgccaga agagcgccag ccgtaagatc cggcaaaaat
     4861 ggccgatggt ccgttagctt tacgaatacg atcgtgttgc tcatgaatca gttttaatgc
     4921 ttgttcccag ctcacctgta cataggtatc ttcgcctcga cctttcgccg gttgcagtgg
     4981 attatcgaga tagctttttc tcaccatcgg atgctgaata cgcgccgtgg tgtgtacctg
     5041 atccgccgcc gtagactgta aggagttcgg tatggttttc gccagcgcgc ctgtcgaaga
     5101 aacaatcttg ccgtccttca cttctacgtt catcgctccc cagcgtcccg cggtgaggat
     5161 tttaccgccc ttctcttctg cccatgcggg tagcggtgct gccgatgtca ccaccagcgc
     5221 tccagcggca ataccgctgt gtttaataaa ttcacgtctt gttaatgtca taacttcctc
     5281 cctgatcaac gaggatcact gtttctcggt aatatctttg gcgttgtact ggaaataccg
     5341 cgttaaaatg tccagttcgt tttcgctcat gctggttcgt gcccccattc ctttggcaat
     5401 ggacggccac gcattgacgg tgtaatggtc ggcggcaata ggggcatgac aaccagcgca
     5461 ataggtatcg tcaagttttt cagcgtattg ccatagcggt ttacggtccg ctaatgcggg
     5521 atcggtaagc gcaccctgta aagacgcctg acgccattga ttgccgtatt cgtcagcctg
     5581 ccattccccg tttaccgtga gcgccttgat accttcttca cttaatgtgg ctagcgccag
     5641 ccgttgacct gccgccaggt agagcgtgtt ttcactgccc tgcatttgat aaccctgcaa
     5701 cagaacgatc ggctgtttgc cactggcatc aacgacggtg agatcggtac caggattcac
     5761 ggtagccagc tcgcctatgc gagaagtttt gaaaggataa atatgtgcgc cattagtcac
     5821 tgaagtagcg gtctgacttt ccagctcatg cgccgcgttg tcatccattt ttatttctgg
     5881 cggaaaatgg gcaatgcctt tatgacaatc gatacaggtt tcgccgccct tttgtgcttt
     5941 gttatgcatt ttctgcgcag attcactttg cgaggcaata tccatggcat caaaagaatg
     6001 gcaactacgg cacgttgcag agtcagttgc ttttaattct ttccatactg tttcggccat
     6061 ttcctggcga tgagtttcga acttatcgtc actgtctatt ttgccgctaa caaattcatg
     6121 ataaatatct ttagatgctt ttaatttagc aaataaataa tccatccctg actttggaat
     6181 atggcaatcg gcacattccg cacgtatccc tttctggttc gaaaagtgga cagttccctg
     6241 atattcctca aaaggtttac tcatcgagtg g
//
LOCUS       DAJOSY010000169         6218 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJOSY010000169 DAJOSY010000000
VERSION     DAJOSY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.169"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..535
                     /locus_tag="OHN27_004100"
     CDS             <1..535
                     /locus_tag="OHN27_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCQ2288445.1"
                     /translation="RPGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLAT
                     EMGADLAINSHTEDAAKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLP
                     PESMSLDIPRLVLDGIEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFT
                     EMEEGKIRGRMVIDFRH"
     gene            681..965
                     /locus_tag="OHN27_004101"
     CDS             681..965
                     /locus_tag="OHN27_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCQ2288446.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(1194..1847)
                     /gene="fdnI"
                     /locus_tag="OHN27_004102"
     CDS             complement(1194..1847)
                     /gene="fdnI"
                     /locus_tag="OHN27_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCQ2288447.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(1840..2724)
                     /gene="fdnH"
                     /locus_tag="OHN27_004103"
     CDS             complement(1840..2724)
                     /gene="fdnH"
                     /locus_tag="OHN27_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCQ2288448.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(2737..5784)
                     /gene="fdnG"
                     /locus_tag="OHN27_004104"
     CDS             complement(2737..5784)
                     /gene="fdnG"
                     /locus_tag="OHN27_004104"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5197..5199),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCQ2288449.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQSEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            6016..>6218
                     /locus_tag="OHN27_004105"
     CDS             6016..>6218
                     /locus_tag="OHN27_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCQ2288450.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAG"
BASE COUNT         1453 a         1706 c         1569 g         1490 t
ORIGIN      
        1 tcgtccaggg cagtggattg ctatctacgg tcttggcggt ctgggtaacc tcgccctgca
       61 atacgcgaag aatgtcttta acgccaaagt gatcgccatt gatgtcaatg atgagcagtt
      121 aaaactggca accgaaatgg gcgcagattt agcgattaac tcacacaccg aagacgccgc
      181 caaaattgtg caggagaaaa ctggtggcgc tcacgctgcg gtggtaacag cggtagctaa
      241 agctgcgttt aactcggcag ttgatgctgt ccgtgcaggc ggtcgtgttg tggctgtcgg
      301 tctaccgccg gagtctatga gcctggatat cccacgtctt gtgctggatg gtattgaagt
      361 ggtcggttcg ctggtcggca cgcgccagga tttaactgaa gccttccagt ttgccgccga
      421 aggtaaagtg gtgccgaaag tcgccctgcg tccgttagcg gacatcaaca ccatctttac
      481 tgagatggaa gaaggcaaaa tccgtggccg catggtgatt gatttccgtc actaagaggc
      541 ctttgctgcg actgccatgt tcgggtcgca gcatcgcaca ctctccaaca tgaaatggct
      601 aaatggatta ttgttgcgta ggaaggaaca ataatgatag tgttcccagt gaaggaacgc
      661 tactgagagt aaagacacta atgaaaatgg ccaatcatcc ccgcccgggg gacattattc
      721 aggaatcact ggacgaactt aatgtcagcc tgcgcgagtt tgccagagca atggaaattg
      781 ctccctcaac ggcaagccga ttgctgacag gaaaagcagc tttgacgcca gaaatggcga
      841 ttaaactttc cgtggtgatc ggcagttcgc cgcaaatgtg gctgaatctg caaaacgcct
      901 ggagtctggc agaggcagaa aaaacagtgg atgtgtcgcg attacgccgt ctggttacgc
      961 aataaaaaaa tggcacttta taaagcgcca tttagaactg atgacaaacg caacattgcc
     1021 tgatgcgcta cgcttatcag gcctacacgt tcactgcaat ctattggatt tgaagggttt
     1081 tcatgccgga taaggcgttc acgccgcatc cggcatgaac aaagcctacg ttgtctacaa
     1141 tatgaaaatg gcgcctttta cagcgccatt ttcagcttaa agttcaaaag agattatatc
     1201 ccttcttcac tctctttttt cgcttctgcc ttctcgattt cacgatacca gcgcggatgg
     1261 tgtttcttcg cccagcgacg acttaccttc ccttcgatca tccctttaat cgatcctttc
     1321 acccaaaatg ccatatacat atggatcagg atggcgtgga tcaggatgat acccgcagcc
     1381 gcgtggatca gcaggctgta gcgaacaacc tgcatcggga agtactgcgc aaagtacgga
     1441 cgccagataa tcaccccggt caccagcagc acgaaaatca tgctcatgat cgaccagaac
     1501 atcatctttt gcccggcgtt gtacttaccg acatccgcca ctttatgctc attgcctttc
     1561 aatacttcga caatgttcaa cagccacgga atatctttct tatccgggat gttgtgatgc
     1621 acaaaacgca caaacataaa catcagtgcg acgaaaatcg caatgccgaa gaacgggtgc
     1681 aaaatgcgtc ccatctgcgg cgtaccgaag gtttgcgtca gccattgcag cgtcgggaag
     1741 aagaacgaaa tcccggacag cgccaccagg aagaagcaaa tcaccacggt ccagtgacag
     1801 gcgcgatcaa taaatttggt gcgcacaatc attttcgact tactcatgat gatcctcctc
     1861 gtcatcgtcc acttccttat tcgggccaat accgatgtag tggaaaatca acccggcaaa
     1921 agtggcaata aagccagccg ctgccagcgg tttcaacgcg cctttccaca ggcttaccga
     1981 ggtgtcgatc ttcggatctt tcggcagacc gtgatacagc tccggctgat cggcgtgatg
     2041 cagcacgtac ataacgtgcg taccaccgac cccttccggg ttgtagacgc cagcatgttc
     2101 gtaaccacgc gctttcagtt tcgccacgcg ctgttccgcc agctccagca tctccttctt
     2161 ggtgccgaag tggatagccc cggtcggaca ggttttcaca caagccggtt cctggccgac
     2221 gctgacgcga tcgacgcaga gcgtgcattt atatacccgg ttatcctctt tgttgaggcg
     2281 cggaatatta aacggacacc cggcaatgca gtaaccacag ccgatgcagt tttccgactg
     2341 gaaatcgaca atcccgttag cgtactgaat gattgcacca gcagacgggc acgccttcag
     2401 gcagccggga tcttcacagt gcatacagcc gtctttacgg atcagccact ccagcttgcc
     2461 gttctgttcg gtttcgctaa agcgcatcac cgtccaggac ttggcgctca gatcggcggg
     2521 gttatcgtaa accccgacgc agtgccccac ttcatcacgg atgtcgttcc actccgaaca
     2581 cgccacctga caggctttac agccgataca ggtggaaacg tcgataagtt ttgcgacttc
     2641 tgctttgtaa tcacgcacct gagaaggcgg cgtgatggag ttagttgcgg accttttgat
     2701 aatgtcctgc gtttccatag ccatctgttc gcccccttac gccttctcga tgttgactaa
     2761 gaacgcttta tattccggcg tttgcgagtt tgcatcaccg acattcggcg tcagagtgtt
     2821 agcgatataa cctttacgcg cgacaccctc aaagccccag tggattggaa tacccaccgt
     2881 ttcaacctgc tgaccattta cattcagcgg tttcagacga cgcgttacca cagccaccgc
     2941 gcggataaag ccacgcttgc tggagacagt gacacgatcg ccattattaa tgcctttcgc
     3001 cgccgccagc gtttcgctga tttccacaaa ctgttccggc tgagcaattg cgttgagcaa
     3061 tgcgtgcttg gtccaggtgt ggaagtgctc ggtcagacga taggtcgtac ccacatacgg
     3121 gaactgctct tttttaccca tccgcagcgc gtcttgttca tacagacgaa caaccgggtt
     3181 agacaccacg ttcggatgca gcgggttagt gcccagcggc gtttcaatcg gctcgtagtg
     3241 ttccgggaac ggaccttccg ccattttgtt gatggcaaac aggcgtccca tcccttccgg
     3301 ctgcatgata aacggcccgg ttggcgtacc cggtgcggca ttgccgaagt caggaatatc
     3361 gttacccgtc cacttgctgc cgttccactg gatcagcatc cgtttcggat cccacggttt
     3421 accgttgata tccgccgaag cacggttgta cagcacgcga cggttgagcg gccacgccca
     3481 ggcccatccc agcgtattcc ccagaccgga cgggtcggag ttatcgcggt tagccatctg
     3541 gttgccctgc tctgtccagc taccggtgta gatccagcaa gaagatgcgg ttgtaccgtc
     3601 atcacgcaga tgcgcaaagc tactcagcaa ctgacctttc ttcgcaataa gcacgccatt
     3661 agcgtcatag agatcttcca gcgcgtagcc gttgttctct ttagccactt cgtcagattg
     3721 cggttcgtgc ggctgcttgt agttccagct catcttcatc agcggttcta cgcctttacc
     3781 accttcggac tggtacagct cgcgcagatg atggtagata cccgccagaa tttcaccgtc
     3841 gttacgcgct tcgcccggcg cgtcctgacc tttccagtgc cactgcaacc agcgaccgga
     3901 gttagcgata gaaccatctt cttcagcaaa gcaggtcgaa ggcagacgga atacttcagt
     3961 ctgaatagac gccggatcga catcgttcga ctcaccgtgg ttctgccaga aggtagaggt
     4021 ttcagtcacc agcggatcga taaccaccat gtacttcagc ttgctcaggc agctcaccac
     4081 tttgtttttg tccgggaagg acgcaaccgg gttaaagccc tggcagaaat aaccggtgac
     4141 tttgccttca tccatcatgt tgaaatactt gatgacgtcg taggtctggt cccacttcgg
     4201 cagccagtca tagccccagt tgttctcttt ctgcgcggca tcgccataga aagatttcat
     4261 caggctaacg aagaacttcg gatagttgct ccagtagttc acctgatcag ccagcgtcgc
     4321 tttcggcgtg ttcgcttcca gatacgactg caaatcaacc tgtttttctg acggcagcgt
     4381 cagataacct ggcaggctgg tagagagcag gcctaagtca gtcaagccct gaatgttgga
     4441 gtgaccacgc aatgcgttca cgccgccacc ggccataccc atgttaccga gcagcaactg
     4501 gatcatcgcc atagtacgga tgttctgcgc acccacagtg tgctgcgtcc agcccagcgc
     4561 gtacaggaag gtggttgtgc gatccggtgc gctggtggag gccagcactt cacacacttt
     4621 caggaagtcg gcttttggcg taccgcagat gttttcaacg acgtccggcg tgtagcggga
     4681 aacgtgctct ttcagcaggt tccacacaca gcgcggatga gtcagtgttt catcgcgttt
     4741 cgcatagccg ttttcatcga actgatagtt ccaggacgat ttatcgtatt gacgtttttc
     4801 agcgtcgtag ccgctgaaca gaccgtcttc gaaagcaaaa tcatcacgca ccagcaggct
     4861 ggcgttggtg taatgcttaa cgtattcggc gttgattttg ttgttttcga tcaggtagcg
     4921 caaaacgcca gacaggaacg taatgtccgt accggaacga ataggcgcgt aaatatccgc
     4981 cacagaagcg gtacgcgtaa aacggggatc gacaacgatc aaggttgcgt cgttgttgtt
     5041 tttcgcttcc atcgcccagc ggaaaccgac gggatgcgct tcagcagcgt taccgcccat
     5101 caccatcacg acgttagcgt ttttgatatc cacccagtgg ttggtcatcg caccgcgacc
     5161 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acgcgcgcct ggttgtctac
     5221 cgccagcatc ccgagggagc gggcaaattt ctgggtcagc atcccggttt cgttgctggc
     5281 accggaggca cacagcatac cggtagaaag ccaacggttt accgttacgc cctgctcgtt
     5341 cttttcaata aagttagcgt cacggtcagc tttcatcagc ttcgcaatac gggagaatgc
     5401 ttcttcccag ctaatgcgct gccatttgtc agaaccgggc gcacgatatt ccgggtagcg
     5461 cagacggttt tcgctgttga cgtaatccag caaaccggcc cctttcgggc acagcgcacc
     5521 acggcttacc ggatgatccg ggtcaccttc aatgtgataa atcgcttctc tggcgttttt
     5581 tgcgccatca cccaggctat acatcaatag cccgcaacct acggaacagt atgtgcaggt
     5641 gttacggatc tctttagcgc gtaataattt gtagtttcgc gcctgagcca gtgcttgctt
     5701 cggggcaaag cccaatgccg ctactgttgt tccagccata ccgcccgcgc agattttaaa
     5761 aaattgtctg cgactgacgt ccattgcttt cctctttttt caggggtatt actgcgcgag
     5821 gaaactaaca gtaaaattct gaatcttttt gcgcgaaatc aaaaagaaca tctgtttgcc
     5881 acaaaataat ccgcctgcgc aaattgacct acctcaatag cggtagaaaa acgcaccact
     5941 gcctgacagg ccagttaaaa aaatgctata aaattcagct taatttttaa cggcaagaga
     6001 gacaaaacag cgagcatgac acgacaaaaa gcaacgctca tagggctaat agcgatcgtc
     6061 ctgtggagca cgatggtagg attgattcgc ggtgtcagcg aggggctcgg cccggtcggc
     6121 ggcgcagctg ctatctattc attaagcggg ctgctgttaa tcttcacggt tggatttccg
     6181 cgtattcggc agattccgaa aggctattta ctcgccgg
//
LOCUS       DAJOSY010000170         6210 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJOSY010000170 DAJOSY010000000
VERSION     DAJOSY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.170"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1433
                     /locus_tag="OHN27_004106"
     CDS             <1..1433
                     /locus_tag="OHN27_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCQ2288451.1"
                     /translation="TLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGY
                     YEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTVRVVGVVKKALHVQMDGLETVLAAM
                     CEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALARRK
                     AAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFPSTMVDRIVPA
                     VTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEE
                     MKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAPTLKVQGVDLQ
                     DYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLTHDSKFDLLALGVAGW
                     MRYVGGVDEQENPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPANSLF
                     TAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1469..1672)
                     /gene="ydfZ"
                     /locus_tag="OHN27_004107"
     CDS             complement(1469..1672)
                     /gene="ydfZ"
                     /locus_tag="OHN27_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCQ2288452.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1849..2535)
                     /gene="ydfH"
                     /locus_tag="OHN27_004108"
     CDS             complement(1849..2535)
                     /gene="ydfH"
                     /locus_tag="OHN27_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCQ2288453.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2624..3370)
                     /gene="ydfG"
                     /locus_tag="OHN27_004109"
     CDS             complement(2624..3370)
                     /gene="ydfG"
                     /locus_tag="OHN27_004109"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCQ2288454.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKVEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3507..5552
                     /gene="dcp"
                     /locus_tag="OHN27_004110"
     CDS             3507..5552
                     /gene="dcp"
                     /locus_tag="OHN27_004110"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCQ2288455.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5597..6115)
                     /locus_tag="OHN27_004111"
     CDS             complement(5597..6115)
                     /locus_tag="OHN27_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCQ2288456.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1538 a         1493 c         1591 g         1588 t
ORIGIN      
        1 cgacactccc tgtttatgat cgtaataacc tggccccaag aattgttcat ttaggctttg
       61 gtgcatttca ccgtgcgcat cagggcgtgt atgccgatat tcttgctaca gaacatttca
      121 gtgactgggg atattatgag gtcaacttaa tcggcggcga acagcaaatt gccgatttac
      181 agcagcaaga taatctttat accgttgcgg aaatgtcggc cgatgcgtgg acggttcgcg
      241 tcgttggcgt cgttaaaaaa gccttgcacg tacagatgga tggcttagaa accgtgttgg
      301 ctgcgatgtg tgaaccgcaa atcgcgattg tctctctgac aatcaccgaa aaagggtatt
      361 tccactctcc ggcgaccgga cagttaatgc tcgatcaccc gatggtcgct gccgacgtgc
      421 aaaatcccca ccagccgaaa acagcaacag gggtgattgt tgaggcgctg gctcgccgta
      481 aagcggcagg acttcccgca tttaccgtca tgtcatgtga caacatgcca gaaaacggtc
      541 atgttatgcg tgacgttgtc acttcctacg cgcaagccgt tgatgtaaaa ctggcacaat
      601 ggatcgaaga taacgtgact ttcccatcaa caatggtgga ccgtattgtg cccgcagtga
      661 cagaggatac gctggcgaaa atcgaacaac ttaccggtgt gcgcgatcct gctggcgttg
      721 cctgtgaacc tttccgccag tgggtaatag aagataattt tgttgccgga cgtccggaat
      781 gggaaaaagc gggagcagaa ctggttagcg atgtactgcc ttatgaagag atgaagttgc
      841 gcatgctcaa cggcagtcat tcattcctgg cgtatttggg ttatcttgcc ggatatcagc
      901 acattaatga ctgtatggaa gatgaacatt atcgtcatgc ggcgtatggc ttgatgttgc
      961 aggaacaagc gccgacgctg aaagtgcagg gggttgattt gcaggattac gctaaccgat
     1021 taattgcacg ctatagcaac ccggcgttac gtcatcgaac ctggcagatt gcgatggatg
     1081 gcagccagaa attgccacag cggatgttgg attctgttcg ctggcatctg acgcatgaca
     1141 gcaagttcga tctgctggcg ctgggcgtcg cgggttggat gcgttatgtc ggtggtgttg
     1201 atgaacagga aaatccgata gaaatcagtg acccactgtt acctgttatt cagaaggctg
     1261 tacaaagtag tgccgaaggg aaagcgcgcg tccagtcatt gctggcgatt aaggcgatct
     1321 ttggtgatga tttgccagcc aatagcttgt ttactgcaaa agtgacggaa gcgtacttgt
     1381 ctttattagc gcatggcgcg aaagcgaccg tggcaaaata ttccgtgaag taaaaaaagc
     1441 aatgccgcca tcggcggcat tcacacgctt agttcatgcc gagacgaatc aggtccagtg
     1501 gtgccagttt ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg atttgctcag
     1561 cggtcagacc ttcagtatca atcgacagga tcttgccagt gtgaccggtg ccgctaacca
     1621 taacacggct gccagtggtg attgcgttac ggttacgatc gtaggtcgtc ataggtgttt
     1681 tctcctttct gatttacagt cgaagcccac gtaacgggcg caatataaat actcctgtcg
     1741 agtgaaatat tttttgtttt tgatcaagct cacatctttt ttgatgcatg catgagccat
     1801 aaggcggggg aaaagactct caccccggat gggatgagag gaaatgaatt actcttcgct
     1861 aaaccagtcg ctattttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg
     1921 ttgcgtcatt gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc
     1981 aagatgctgg cgtaacagca tttctggtgg agaaacgtgg tcgaaactca tatagcgcac
     2041 gcgatcaacg gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat
     2101 ctgcgtcagg agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg
     2161 ctcaatggca atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt
     2221 aatcatgctc gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc
     2281 gttgcgcacc tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg
     2341 aatcaggccg ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt
     2401 gaaacgaaca gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac
     2461 aatgtcgcga cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg
     2521 cgtttcgacg gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt
     2581 tttgtgacaa gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat
     2641 tcagtccggc atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag
     2701 caggcagcgt tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg
     2761 tattttgata ggttttttcc actttaccgt catcgccttt aaagcggaca ttggaaaact
     2821 cggtgccacc caccagaccc ggctcgatgt cggtgacgcg caccgccgta ccatgcagat
     2881 ccgtacgcag attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac
     2941 caccggcata cggccagcta cctgccgttg agccaatgtt aataatatga ccatgattac
     3001 gttcaaccat acccggtaag acggcgcgcg tcatatatac caggccttta ttgttggtat
     3061 caatcatcgt ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca
     3121 ggccggcatt atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca
     3181 tctcttcaat agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca
     3241 gttcgtcttt taactcctgc aaccgttcct gacgacggcc agtggcgata actttatgcc
     3301 cttgttgaat aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta
     3361 aaacgatcat ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgggatgct
     3421 taagagcggg taatactgga ttgcaagatt gcactccgtt atcagtaagc ctacgctatg
     3481 gaacacctcg cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca
     3541 cactgccgta tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat
     3601 tcgatgaggg aatgcagcaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa
     3661 cgcctgattt caacaatact attctggcac tggaacaaag cggagaatta cttacccgcg
     3721 ttaccagcgt cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg
     3781 atgaacagtt ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat
     3841 tattcgcgcg ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat
     3901 ccatccgcct ggtggaggtg attcatcaac gttttgtcct tgccggagcc aaacttgcgc
     3961 aagctgataa agcaaaatta aaagtactga atacagaagc tgcgacactg accagtcagt
     4021 ttaaccagcg gttactggca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc
     4081 aactggcagg aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag
     4141 gtctggataa caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg
     4201 aaatgcgcga tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa
     4261 aaaatgatgg caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc
     4321 aggcgaaact gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa
     4381 aaacacctga agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg
     4441 caagcgatga attagcctcc atacaggcgg ttatcgataa gcagcaaggc gggtttagcg
     4501 cgcagccgtg ggactgggca ttttatgccg aacaggtccg gcgggagaaa tttgatcttg
     4561 atgaggcgca gctcaagcca tattttgaat taaacacggt gttgaatgaa ggtgtattct
     4621 ggaccgcgaa tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt cctgtctacc
     4681 atccagacgt tcgggtgtgg gaaatttttg atcataatgg cgtggggctg gcgttatttt
     4741 acggtgattt cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg
     4801 agcaatcaac gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac
     4861 ccgctgccgg tgagcctgcg ttgttactct gggatgatgt cataacctta ttccatgaat
     4921 ttggtcatac actgcacggc ctttttgccc gccagcgtta tgccacgctt tccggtacca
     4981 acacgccgcg tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc
     5041 cgcaggtatt cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac
     5101 tgcaacagaa aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc
     5161 ttagcgccgc acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg
     5221 atgtcgatga tttcgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac
     5281 cgccacgcta tcgcagcagt tatttcgccc atatttttgg tggcggatat gccgcaggtt
     5341 attacgctta tctgtggacg caaatgttgg ccgatgatgg ttatcagtgg tttgttgagc
     5401 agggcggatt aacgcgtgaa aatgggctgc gttttcgcga ggcgatcctt tccagaggta
     5461 acagcgaaga tctggaacgc ctgtatcgac aatggcgcgg taaggctcct cagattatgc
     5521 cgatgctgca acatcgtggc ttgaacatat aaacaccacc aatggctggc ttggagatat
     5581 cgccaggcca gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcactg
     5641 ctaaagctaa taccgtttcg cagtcgccag caaaatgcat aaccgcagtg taggtctggc
     5701 ctttaatatg ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg
     5761 gtccgccgta gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg
     5821 ttgccatttc tgctgccact ttctcactca ccgcagaata tcgttcaaga gattgctggc
     5881 ttacgctgag gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa
     5941 atttgggtgt atcttccgct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt
     6001 cggcggtagt taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt
     6061 tttcaatagt gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt
     6121 tttgtccacg ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa
     6181 taattttcaa acatataaat ccgattattc
//
LOCUS       DAJOSY010000171         6143 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJOSY010000171 DAJOSY010000000
VERSION     DAJOSY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.171"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(60..575)
                     /gene="ogt"
                     /locus_tag="OHN27_004112"
     CDS             complement(60..575)
                     /gene="ogt"
                     /locus_tag="OHN27_004112"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCQ2288457.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(586..2112)
                     /gene="abgT"
                     /locus_tag="OHN27_004113"
     CDS             complement(586..2112)
                     /gene="abgT"
                     /locus_tag="OHN27_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HCQ2288458.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPQNGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGISPRLS
                     "
     gene            complement(2149..3594)
                     /gene="abgB"
                     /locus_tag="OHN27_004114"
     CDS             complement(2149..3594)
                     /gene="abgB"
                     /locus_tag="OHN27_004114"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCQ2288459.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNMTPSPLK"
     gene            complement(3594..4904)
                     /gene="abgA"
                     /locus_tag="OHN27_004115"
     CDS             complement(3594..4904)
                     /gene="abgA"
                     /locus_tag="OHN27_004115"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCQ2288460.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YMVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            5080..5988
                     /locus_tag="OHN27_004116"
     CDS             5080..5988
                     /locus_tag="OHN27_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2288461.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
BASE COUNT         1565 a         1587 c         1552 g         1439 t
ORIGIN      
        1 agttgttaag ggtaacttta cataaaagtg tgaacaagct ggcacaaatt gtttaatgtt
       61 tacagcaaaa gataaccttc atggcgcaat aaccactctt ttcgctgaac tccgcctgca
      121 tatccggtca tggtgccgtt tcggccaata acccgatggc aaggtacgac gatgctgatg
      181 ggattcgatc cgtttgccgc accaacggca cgcgccgcgc cagggcggcc caattgttca
      241 gccagttggc cgtaatgcat tacctgcccg caggggatag tgcgtagtgt tttccagact
      301 tcgcgctgaa atggcgtccc ccctgtggca gtgggaagcg tatcaataat gctaagatta
      361 ccggcaaaat aatcacgaag cttgtcgctt aaaccgcctg ggttggtggc agaaatgcgc
      421 tcatagcctt ctttgcgata atggatgtcc agcagctgca ccatgcgttc gctgtactct
      481 tcccattcaa ccgcccgcag acgaaattgt tcatcgcaaa tcacccacag tggacccagt
      541 ggcgtggcaa ttttttcttc aagtaatctc agcatccgtt ctctcttaag acaaacgtgg
      601 ggaaatcccc ggacctatcg gcagaccaac aagataccac gccagcaaca tcagcagcca
      661 taccaccaaa aagataagcg gatagggcaa gactaacgaa tagtaagtac ccagtttcgc
      721 gtctggtttg tagcgttgca ggaatccaag aaacagtgga acaaaaggag ataccggcgc
      781 taaaggcaat acggatgagt cggcaatacg aaagaggatt tgcgcaaatg ccgggtgaaa
      841 gccaagtagc ataaacattg gtacgaaaat gggggcaaga atcgaccaga ttgcggaacc
      901 gctggcaata aacatgcata agaaagagga aagcaacgcc agaccgacaa acgccgggat
      961 gccgctaagc cctgaacttt ccagtatatc ggtcagcccc acggcgatga atttccccat
     1021 gttgctccag ttaaacatgg cgacaaattg ggcgagggga aaaaccatca cgataaatcc
     1081 cgccatctct ttcatcggtt caatcattaa atgcggtaaa tccgcctgac gtcgaattgt
     1141 gcgggtagcg atgccataag ccagcgagac aacaaagaaa aaaagaatga tcagtggcac
     1201 gataccttta ataaagggtg atggcatcac ggtgtgatta atcggatcgc gcaatatccc
     1261 gttttgcggg atcaccatca gcgcaatcgc agcaataaaa agtagcgata cgacacctgc
     1321 tatgcgtaaa ccaaaacgct gactttcggt caatgtctgc agtttctcat cgctgtttcc
     1381 ctgccattga cctaaccgtg gctcgatgat tttgtcggtt atcaggccgc caacaatcgt
     1441 cagtacgact acggagctgg ccataaaata ccagttatca attacactga cgtgcatttg
     1501 cggattgaac gcagctgccg cttccgtgct gatccccgac agcaacacgt cggttgtgac
     1561 aatcagtaaa ttagccgtaa agccgcaacc tacgcctgca atggcagcca gtaaacctgc
     1621 aactggatgc ctgcccaccg ccagaaaaat cagcgcaccc atcggtggca tgatcactaa
     1681 cgccgcatcg gaagaaatgt ggctgaaaaa agcaataaac agcaccatat aactggcgta
     1741 gcgggcatta acatgcgatg ccattttaac cattagtgct ggcagtaagc cgacgcgctc
     1801 cgccagaccg gcacctaaaa ccagcgccag gatcgcacca agtggagcaa aaccgctaaa
     1861 gtttttaata acattgggta aaaaccagtg taatccttcc acactgagca ggtttttcac
     1921 caccaccggc gtaccatcgg tcgggttttt cgcactgacg ccaaaggccg acaaaattgc
     1981 cgtcgtcacc atgagtacga taatcaaata gataaagagc agaaaaggat ggggaacctt
     2041 gttaccaatt ctttcgaccc agccatagag cttcccggat tgggaggacg acggtatgga
     2101 tgacatactc atgggcattc ctcggttgtt gtgtttgcgt tgttgttgtt attttaaagg
     2161 tgacggtgtc atgtttttcg ggatagggca gtgatacggt tgcgtgtccg ttacttgctg
     2221 atgctcttgt tggcattctt gcaatagccc tgaatcaagg aagagattga ctgtggttgc
     2281 tgccatagtt ttcgccgcca gcagcattcc tttatgagca atagatgttc gcccctggct
     2341 aaccagttgc cacgtatgta gcggtgtacc gacggcaaaa caggggctga aacactgggc
     2401 aacaggcagt ttccaactga cgtcgccgac atcagtcgat gccgcaagca cgttatcggt
     2461 ggcggcatat ggagcgactt cattcgccag taccgtttca cgatgacgta gtgcaaaaac
     2521 cttgccgttt tcgccaccgg ttgcggcgat attattcaga ctgttttgcc gatcgttgga
     2581 ggtgagcgta gcctgaattt gtttcgcaaa agccagttct tcggagttcc attccggggt
     2641 accaaaatgg gatagggcct ggtacatggc attttctaag gtgcgattcg ggagataact
     2701 ggaacaggct ttgtcgaagc ggcattcaac cgtggtttcg gtcatcaatg ccgcaccttc
     2761 ggcgattttg gcgacccgat cataaatatg ctgcacgtcg gtcatttcgg gggcgcggat
     2821 aagataaagc acttctgcct gcgcctggac cacgttgggc gagatcccgc cgctatttgt
     2881 gatggcatag tgtacgcgcg ctttttcaat aatatgttcg ttgaggaagt tggtgccagt
     2941 ggtcatcaac gttacggcat caagggcgct gcgtcccaaa tgaggggaat tcgcggcatg
     3001 tgctgcgatc cctttaaagc gccatgatgc ctgaatgttt gccagcgtgc gggtattgaa
     3061 cataccggca aaggcttccg ggtgccaggt gagtgccgca tccacatcat caaatacccc
     3121 ctcgcggacc atgaacgttt tacccgagcc gccttcttcg ccaggacaac cataaaagcg
     3181 cactgtgccg ccttgcccat attgttccag ccatttcttg acggctattg cagcggcaaa
     3241 ggcggcggtt cccagcaaat tgtgtccgca accgtgacca ttttcaccgg gcgtcgcgga
     3301 tgtaggttgc gcgcaaccag cttgctgact taaacctgcc agggcgtcat attctcccag
     3361 cagggcgata accggtttgc cttgaccaaa cgaagcaata aaggcatttg ggatattgcc
     3421 tacgttgcgg gtaacggtga agcctgcaga ttccagcgcc gaagccagat gctccgctga
     3481 ccagaactct tcaaaacgtg tttctggatg atcccagatt tgatcggcaa tatcggtata
     3541 acgttggcga tcggcttcaa tcgcatcgtc gataaaacga tagatttcct gcatcagata
     3601 cctcgcgtcc agggaaaatt gagcgcggtg cgcgccagcg tttcgacggc aatagcgaga
     3661 acctgctcgt caaaatcgaa tttttcgttg tgatgacctg ccgccagctg tgtgccaaac
     3721 accatgtagg aggcttgccc ttgatgttgc tgcacgcggg ccatcattaa tgtggcatct
     3781 tcggaacccg caggcgcttc aacacgttca atggcctgat tgacccccgc gacctgagcc
     3841 gcctgacttt gcaaccatgc gacccattgc ggcgaaggag aactggcggt agctgcaccc
     3901 atcagacgag tttcaacgcc gacaccatac atggttgctg cgccctgaat cgcttgctga
     3961 gcacggtcaa aaacatattg attaatgacg tcgctggccc cgcgtgtttc cactttcagc
     4021 aacgccgagg caggaacaac attacgaccg cttcctgcct gcataacgcc cacgtttact
     4081 ctggaagcgc cttcgctgtg cggggcgatt gcatgtagtg caagagttgc ttgtgctgcc
     4141 gccaacaagg cattgtgacc gtcttctggt tttgcgcctg cgtgagcggc ggtcccggta
     4201 aagtgcgcgt caaatttggt ggttgccata aaattatcac tgccgcacac cacggtgccc
     4261 gcaggtacgc cagtgccaat gtgcacggca gtaaaataat caacatcatc cactacacct
     4321 gcatcgacca tcgcccgcgc gccacgcgta ccttcctctg caggctgaaa aatcagtttg
     4381 atgacgccat gtagtccgga ctcgaactgt ttaagggtat gcgccagccc aagcccaatg
     4441 gcggtatgtc catcatgacc acaggcatgc atcattccgg cgttacatga cgcaaaaccg
     4501 tcgcggtagg ggcgatggct gacatcctgc tcttcactga gatccagcgc gtccatatcg
     4561 acacggaaag ccatcaccgg accggggcga ccggtatcca gggtggcgac gataccagtg
     4621 aaaccacctt caaaagccgc aatccattgt gctagcgcac cctgttgacg agcgcgctcg
     4681 aattcgcgtt gtagagtgaa ttcatcaggt aatcccatcc ggctactttc attaactact
     4741 tcgcgaccca gcgccagtga atagccgagc tggtgcaatt cttccgcaac aagggtggca
     4801 gtgcggaatt ccacccagcc agactctgca tagtgatgaa aatcacgtcg ccagtgcgat
     4861 aattttgggg caagcgaatt aacaaattga ttcaaagact ccataacctt tcccgtcatc
     4921 agtaaaaagt gtgacccggt tcacgtagcg atagttttta cttatcacta actgattttt
     4981 cacagtttta accgttcata aattaccctg acacaatcat ctgcattaaa gtagatgcca
     5041 gtttctttgg tctgataaat aacggttatc ggtggcgtca tggcttttca ggtaaaaatt
     5101 catcaaattc gggcttttgt tgaagtggct cgtcagggca gcattcgcgg agcgagccga
     5161 atgttgaata tgtcgcaacc ggcactgagt aaatctattc aggagctgga agaagggtta
     5221 gcggcgcaac tcttttttcg ccgtagtaaa ggcgtgacgt taactgatgc cggtgaaagt
     5281 ttttatcagc acgccagtct tattcttgaa gagctgcgcg cagcccaaga ggatattcgc
     5341 caacgacaag ggcaactggc agggcagatt aatatcggca tgggggccag tatttcccgc
     5401 agtctgatgc cagctgtcat atctcgtttt catcagcagc atccgcaggt aaaagtacgc
     5461 attatggaag ggcaactggt gtcgatgatt aatgaattgc gtcagggaga attggatttc
     5521 accatcaata cctattatca gggaccgtac gaccacgaat ttacttttga gaaattactg
     5581 gaaaagcaat tcgcgatctt ttgccgcccg ggacaccccg ccattggtgc ccgttcgatc
     5641 aaacagttac tggattacag ctggacaatg ccgacgccac acggcagtta ctacaaacag
     5701 ttgagtgaat tgcttgacga tcaggcgcaa acgccacagg tcggtgtagt ctgcgagacg
     5761 ttctcagcct gtatcagtct ggtggcaaaa agcgattttc tcagcatact gcctgaagaa
     5821 atgggctgcg atcccttgca cggacagggg ctggtgatgt tgccggttag cgaaatttta
     5881 ccgaaagcgg cctattattt gattcagcgg cgtgatagtc gccagacacc actgaccgcg
     5941 tcattaatca cgcaattccg gcgagaatgc ggctatctgc aaagttaaaa ctgcataaaa
     6001 aaatagagtc tgtcgacatc cgccagactc tacagtacac acagcagtgc atccgcgtct
     6061 taaatccggt attgtctcct ctgcgccggt gactgtgtta taacggtttt aacctttagt
     6121 tgccaatttt cttcagtgac aga
//
LOCUS       DAJOSY010000172         5943 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJOSY010000172 DAJOSY010000000
VERSION     DAJOSY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.172"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            84..1235
                     /gene="yiaY"
                     /locus_tag="OHN27_004117"
     CDS             84..1235
                     /gene="yiaY"
                     /locus_tag="OHN27_004117"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCQ2288462.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLTHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1400..2938
                     /gene="aldB"
                     /locus_tag="OHN27_004118"
     CDS             1400..2938
                     /gene="aldB"
                     /locus_tag="OHN27_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCQ2288463.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMDEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3157..3300)
                     /locus_tag="OHN27_004119"
     CDS             complement(3157..3300)
                     /locus_tag="OHN27_004119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288464.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3483..3806
                     /locus_tag="OHN27_004120"
     CDS             3483..3806
                     /locus_tag="OHN27_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCQ2288465.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3812..4948
                     /locus_tag="OHN27_004121"
     CDS             3812..4948
                     /locus_tag="OHN27_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2288466.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNIAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4945..5919)
                     /locus_tag="OHN27_004122"
     CDS             complement(4945..5919)
                     /locus_tag="OHN27_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2288467.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1476 a         1412 c         1541 g         1514 t
ORIGIN      
        1 gataatcaat aatactgatc atattttcga atgaaaatcc tcagtaagct gcccgccctt
       61 ttttacactt tcaggagtgt gttatggcat cttcaacttt ctttattcct tctgtgaatg
      121 tcatcggcgc tgattcattg actgatgcaa tgaatatgat ggcagattat ggatttaccc
      181 gtaccttaat tgtcactgac aatatgttaa cgaaattagg tatggcgggt gatgtgcaaa
      241 aagcactgga agaacgcaat atttttagcg ttatttatga tggcacccaa cctaacccaa
      301 ccacggaaaa cgtcgccgca ggtttgaaat tacttaaaga aaataattgc gatagcgtga
      361 tttccttagg cggtggttct ccgcatgact gtgcaaaagg tattgcgctg gtggcagcca
      421 atggtggtga tatccgtgat tatgaaggcg ttgaccgctc tgcaaaaccg cagctgccga
      481 tgatcgccat caataccact gcgggtacag catcagaaat gactcgtttc tgcatcatca
      541 ccgacgaagc gcgtcacatc aaaatggcga ttgttgataa gcacgtgact ccgctgcttt
      601 ctgtcaatga ctcctcgctg atgatcggta tgccgaagtc actgaccgcc gccactggta
      661 tggacgcctt aacgcacgct atcgaagcgt atgtttctat tgccgccacg ccgatcactg
      721 acgcttgtgc actgaaagcc gtgaccatga ttgccgaaaa cctgccgtta gccgttgaag
      781 atggcagtaa tgcgaaagcg cgtgaagcaa tggcttatgc ccagttcctc gccggtatgg
      841 cgttcaataa tgcttctctg ggttatgttc atgcgatggc gcaccagctg ggcggtttct
      901 acaacctgcc acacggtgta tgtaacgccg ttttgctgac gcatgttcag gtattcaaca
      961 gcaaagtcgc cgccgcacgt ctgcgtgact gtgccgctgc aatgggcgtg aacgtgacag
     1021 gtaaaaacga tgcggaaggt gctgaagcct gcattaacgc catccgtgaa ctggcgaaga
     1081 aagtggatat cccggcaggc ctacgcgacc tgaacgtgaa agaagaagat ttcgcggttc
     1141 tggcaactaa tgccctgaaa gatgcctgtg gttttactaa cccgatccag gcaactcacg
     1201 aagaaattgt ggcgatttat cgcgcagcga tgtaatcatc atttccacaa cggctggcaa
     1261 attgtcagcc gctttttcaa ctatctctgt aacccttgcc cgtaaattcg tgatagctgt
     1321 cgtaaagctg ttaccgactg gcgaagattt cgccagtcac gtctaccctt gttatacctc
     1381 acaccgcaag gagacgatca tgaccaataa tcccccttca gcacagatta agcccggcga
     1441 gtatggtttc cccctcaagt taaaagcccg ctatgacaac tttattggcg gcgaatgggt
     1501 agcccctgcc gacggcgagt attaccagaa tctgacgccg gtgaccgggc agctgctgtg
     1561 cgaagtggcg tcttcgggca aacgagacat cgatctggcg ctggatgctg cgcacaaagt
     1621 gaaagataaa tgggcgcaca cctcggtgca ggatcgtgcg gcgattctgt ttaagattgc
     1681 cgatcgaatg gaacaaaacc tcgagctgtt agcgacagct gaaacctggg ataacggcaa
     1741 acccattcgc gaaaccagtg ctgctgatgt accgctggcg attgaccatt tccgctattt
     1801 cgcctcgtgt atccgggcac aggaaggcgg tatcagtgaa gttgatagcg aaaccgtggc
     1861 ctatcatttc cacgaaccgt taggcgtggt ggggcagatt attccgtgga acttcccgct
     1921 gctgatggcg agctggaaaa tggctcccgc gctggcggcg ggcaactgtg tggtgctgaa
     1981 acccgcacgt cttaccccgc tttctgtact gctgctaatg gaaatcgtcg gtgatttact
     2041 gccgccgggc gtggtgaacg tggtcaacgg cgcaggtggg gaaattggcg aatatctggc
     2101 gacctcgaaa cgcatcgcca aagtggcgtt taccggctca acggaagtgg gccaacaaat
     2161 tatgcaatac gccacgcaaa acattattcc ggtgacgctg gagctgggcg gcaaatcgcc
     2221 aaatatcttc tttgctgatg tgatggatga agaagatgcc tttttcgata aagcgctgga
     2281 aggctttgca ctgtttgcct ttaaccaggg cgaagtttgc acctgtccga gtcgtgcttt
     2341 agtgcaggaa tctatctacg aacgctttat ggaacgcgcc atccgccgtg tcgaaagcat
     2401 tcgtagcggt aacccgctcg acagcgtgac gcaaatgggc gcgcaggttt ctcacgggca
     2461 actggaaacc atcctcaact acattgatat cggtaaaaaa gagggcgctg acgtgctcac
     2521 aggcgggcgg cgcaagctgc tggaaggtga actgaaagac ggctactacc tcgaaccgac
     2581 gattttgttt ggtcagaaca atatgcgcgt gttccaggag gagatttttg gcccggtgct
     2641 ggcggtgacc acctttaaaa cgatggatga ggcgctggag ctggcgaacg acacgcaata
     2701 tggcctgggc gcaggtgtct ggagccgcaa cggtaatctg gcctataaga tggggcgcgg
     2761 catacaggct gggcgcgtgt ggaccaactg ctatcacgct tacccggcac atgctgcgtt
     2821 tggtggctac aaacaatcag gtatcggtcg cgaaacccac aagatgatgc tggagcatta
     2881 ccagcaaacc aagtgcctgc tggtgagcta ctcggataaa ccgttggggc tgttctgata
     2941 taagaagctg gtcgcattgg gtattcattg cctgatgcga cgcttacgcg tcttatcatg
     3001 cctacgggaa cctgaccgta ggccggataa ggcgtacacg ccgcatccgg cataaacaaa
     3061 gcgcacgttg ttacaattta cgttttaaac ctacgtgcgc aataacccag actacgccaa
     3121 cagaagtgcg tttctggggc gtgttttgtc aggagtttaa tctcgcgttt cgatggtgag
     3181 cgggcgaacc aggccgtaaa tcaccattgg tgatccgagg ccaatcagca ataaacggta
     3241 gataattgaa ggtttaatac gcttaatctg tactgtggat gttttactgg agttattcat
     3301 tagtttggcc aatagttcca ctatttcaat atgataaacc tcgtcctgtt gaaaaaccag
     3361 acagcacatt tcagaaagta tataaaaaac aatatgtcgt tatataaata aggaaattgc
     3421 actgcatcat ggcatttcat aatatgcgtt aattgttctt ttttatattc tttgagggaa
     3481 atatgttcct ggactatttt gcactgggag tgcttatttt tgtatttctg gtgatattct
     3541 atgggattat tattttacat gatattccct acctgattgc taaaaaacgt aatcatcctc
     3601 atgccgacgc cattcatgtt gctggttggg tgagtctttt tacgctgcat gttatctggc
     3661 catttttgtg gatttgggcc acgctttacc gcccggagcg gggatgggga atgcaaaacc
     3721 atgactcctc cgttgtgcaa ctacaacagc gtattgccgg gctggagaaa cagctcgccg
     3781 acatcaaatc ctcttctgcc gagtaatact tatggattta ctgattattt tgacctatgt
     3841 ggcttttgca tgggcaatgt ttaagatctt caaaattcct gtaaataaat ggaccattcc
     3901 cacagcggcc ctgggtggca tatttattgt cagtggttta attctgttaa tgaactataa
     3961 ccatccgtat accttcaaag cgcaaaaagc ggttatttct attcctgttg tcccacaggt
     4021 gacaggcgtg gtgatcgaag tgacggataa gaaaaatacg ctgattaaaa aaggtgaggt
     4081 gctatttcga ctggacccga cgcgttatca ggcgcgggtg gatcggctga tggcggatat
     4141 cgttaccgca gaacataaac agcgggcgtt gggcgcagag ttagatgaga tggcggcgaa
     4201 tactcagcag gcaaaggcca cgcgggataa attcgctaaa gagtatcagc gttacgcacg
     4261 cggcagccag gcgaaagtaa acccgttttc agaacgcgat attgatgtgg cgcggcaaaa
     4321 ttatctggcg caggaagcct ccgtaaagtc atcggcggcg gaacaaaaac agatccagag
     4381 ccagctggat agcctggtgt tgggtgaaca ttctcaaatc gccagcctga aagcgcagct
     4441 cgcggaagca aaatataacc ttgagcagac gatagtgcgt gcgccgagcg atggttatgt
     4501 gacccaggtg ctgattcgtc cgggtaccta tgccgcgtcg ctgccgctac gtccggtgat
     4561 ggtgtttata cccgatcaga aacgacaaat cgtggcgcag ttccgtcaga actccttgct
     4621 gcgcctggct cccggtgatg atgcggaagt ggtatttaac gctctgccag gcaaagtgtt
     4681 cagcggtaag ctggcagcca ttagtccagc tgttcccggc ggagcttatc agtcgaccgg
     4741 caccttacag acgttaaaca tagcgccggg ttcagatggc gttatcgcga ccattgaact
     4801 ggatgagcac actgatttga gcgctttacc agacggtatt tacgcccagg tggcggtcta
     4861 ctctgatcat ttcagccatg tctcggtgat gcgcaaagtg ctgttacgca tgaccagttg
     4921 ggtgcattac ctttatctcg atcattaatc ttcgctttcc gctgcttgtt caggccgcaa
     4981 ttgctgcgac tgagcaagct ggtggcgaat ttcacgcagc atttcatcca gcacatactg
     5041 gacccgccag ggctggtatt cccgtttacg gaagatagcc accagatcca gccaccattc
     5101 atacgggtgc tgaaagcagg gcaccagcgt gccgtcttgc aggtcgctct gcacactttt
     5161 gtctggcgca aacacaatcc ccaggtgatt tcgtgccagc tccagcgcag attgcgtgtt
     5221 gtcgcaaacg taatttccct tcacgcgata atcccgcacc tctttactcc cggcgacgtt
     5281 gaagcgccag atattcgcgt cgtcgatcat catcgagtcg atcaaaatac aggagtgatg
     5341 ttcgagttcg tcaggacggc taatgggatg tttctcaaga tagcgctggc tggcataagc
     5401 ggttacggca tattgggtaa taaaactggc aaccagcgat tcatcttttg gtggagcgta
     5461 gctgattaaa acatcacaat catcaggaaa ttcgacgcct tcataaaagg cattacgctc
     5521 aagattgcag gtttttagcg ataaggtgat atcaccgata tctttaattt tatcgattac
     5581 atgtttggat aaataggtaa ttatgccggt tggggcgtag atggtgaccc gaccacgttt
     5641 ctcatgctta taatctgcaa taaaattatt aagttgctcg tttctgtcca gcatgtcgtt
     5701 gatgtacggt aacagcgcgg tgccaaaagg tgtgagcatc agctgccggg tggttcggtc
     5761 aaagactttt aaacccactt ttgattcaaa atcagcaaga tatttgctga cgttggcctg
     5821 tgcgatgcca agtacagtcg cggcatggct gatattttca ctggcagcga ttaccgagat
     5881 aatttttaac tcccggtact taagttgtaa ttttgtcatc gcaacagccc aattatatat
     5941 aat
//
LOCUS       DAJOSY010000173         5923 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJOSY010000173 DAJOSY010000000
VERSION     DAJOSY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.173"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1055
                     /locus_tag="OHN27_004123"
     CDS             <1..1055
                     /locus_tag="OHN27_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCQ2288468.1"
                     /translation="KRNLLSSAIIVAIMSLGLTGCDDKKAETETLPPANSQPAAPAPE
                     AKPTEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWL
                     KDFKQGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVA
                     LGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQ
                     LAELKNIEEREGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAK
                     EADKGGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPR
                     ALREYNEMVDDYNSLR"
     gene            complement(1105..2853)
                     /locus_tag="OHN27_004124"
     CDS             complement(1105..2853)
                     /locus_tag="OHN27_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCQ2288469.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTRLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLLG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(2853..3923)
                     /locus_tag="OHN27_004125"
     CDS             complement(2853..3923)
                     /locus_tag="OHN27_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCQ2288470.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEVEKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYEALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            complement(3913..5364)
                     /locus_tag="OHN27_004126"
     CDS             complement(3913..5364)
                     /locus_tag="OHN27_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCQ2288471.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVDSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(5375..5821)
                     /locus_tag="OHN27_004127"
     CDS             complement(5375..5821)
                     /locus_tag="OHN27_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCQ2288472.1"
                     /translation="MAALTASCIALNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1526 a         1518 c         1513 g         1366 t
ORIGIN      
        1 tgaaaaggaa tttattatct tccgctatta tagtcgccat catgtccctc ggtctgacgg
       61 gttgtgatga taaaaaagcc gaaacagaaa cgctcccgcc tgccaatagt caacctgccg
      121 caccagctcc tgaagcgaaa cctactgaag ctcccgttgc aaaagcagaa gctaaacctg
      181 aaacacctgc gcaaccggtg gtcgatgaac aagcggtttt cgacgaaaaa atggatgtct
      241 atatcaagtg ctacaacaag ttacagatcc cggtacagcg cagtctggcg cgttatgctg
      301 actggctgaa agattttaaa caggggccta ccggtgaaga gcgtactgtt tatggcatct
      361 acggcattag tgaatccaac ctcgctgagt gtgaaaaagg cgtaaaaagt gctgtggcgt
      421 taacgcctgc gctgcaacca attgatggcg tggcggtgag ttatatcgat gctgccgtgg
      481 cgttgggtaa taccattaac gaaatggata aatattacac ccaggaaaat tataaagacg
      541 atgcgtttgc gaaagggaaa acgctgcacc agacattctt aaaaaatctg gaagcctttg
      601 aacctgtagc cgaatcttat catgcggcga ttcaggaaat taatgataag cgtcagcttg
      661 cggaactgaa aaatattgaa gaaagagaag ggaaaacatt ccactactac tctctggcag
      721 tcatgatttc agcgaaacaa attaataacc tgatatcgca agataagttt gatgcagaag
      781 cggcaatgaa gaaagtgtct gaactggaaa cgctagtggc gcaggccaaa gaagcggata
      841 aaggcggcat gaatttctcg tttattaatt cggcaggcca gtatcaactc gaggctaaaa
      901 aatacgttcg ccgcatcaga gataaagtcc cgtactctga ctgggataaa gagcaacttc
      961 aggacgcaaa ctcaagctgg atggtcgaag actctttccc gagagcatta cgcgagtaca
     1021 acgaaatggt tgatgactat aatagcctgc gttaaacgtt tttgatgata aatgcagtgt
     1081 cggatgcggc gcatccgaca cagttcaccc cttgtgaagt aactccataa atgcctcata
     1141 tcctgttaac ccggcaataa tctccgccgg atgctgacac aatatgcttg ccagatagct
     1201 aaatattttt agctcatgac gcgcatcggc ggcggcgcag gcgatcaaga catggttaat
     1261 gacttcgtta ttcacctcaa ctggttgggc gagggtaata aaaaatccac gaaaacgtcg
     1321 ctcctgttcg ctccagcaat gtgggatggc gaggcgatta acaatcaggt tttcaccttc
     1381 gccttcgcgg gcgatgatgc gttgtgcttc atcggcggta atatggtgtt gtgcgactaa
     1441 ttgcgcacaa atttgccggg taatatgttg ccagctttca ccgcgtacat tcgaataatg
     1501 aaaacttccg ggggcagaga aaaaacgctc cggttgctgg cgaataaatg ccgtcgccag
     1561 aaaatgtttt atttgttcga taccggcagc cgtaatgata ttttttacgg tgatgtaatt
     1621 atttaccgcg tcatccagta aataatggct gttgttaatg atcaataacg gctcaatctc
     1681 ttcgcgaatg gcaacaagct cgcttaagct acgggcaata atcacccgac aatttaaaac
     1741 atcgcgctca atggcgagct gattaatggt ggcaatcgcg ttctggtccg agagcaaaat
     1801 gatcggctgg cgttcgtttt gatgccgctc cagcgcacag gcaaaataca aaccgagcag
     1861 atcgctgtcg aaaaggggaa tatcgagctg ttcacgcagt agcgtaataa agtgcagact
     1921 catatcaaag gccgcaggcc aggctgcttt caggttattc atgctgcttt ggcggtgctc
     1981 agcaatccac actggtgcgg caaggcaacg taataaatgc ccggtaatgt tctcaaccag
     2041 ccgcgtatca ataatattca atgacgcgat gcccggcacc ctttgcagca accgctgcac
     2101 attatcggca gaaattatct gcgcttgctg atgctgtttc tctatcaaac ctgtcagggt
     2161 tcttacagcg ttatcaccaa gaaacatgcc gctttgttca acgatttgtt ttatctcgtc
     2221 accgggatat tgcggccact catcggtcag ttgattacgc agggcgtaaa tcgccagaat
     2281 caggctggag agataatcac cctgcatgag cggccagcgg tgttggttgt cgcaggccgt
     2341 ggataaatgc tgaaggttgt ctcgtgttat gcccgccagc ggaattaaaa acggatcttt
     2401 gcgcagcaag ttcgccagca agataacgcg tttctcttct gtctcatcaa tgaaatggcc
     2461 caaaccaggg cggctggcca ggcaacaagt gcgttcataa cgctgtttta accggggcag
     2521 acgctctgct acccacgttt ctggcaaatt aagcgccgag gcgagttgcg cacggggagt
     2581 gaaagtattc agtaataaca gcgccagcag ccgatcgtcg ttatcgtgct tttgcagcaa
     2641 ctgaaaaaag ctgcggcgct cgaagatttc cagctgatag cccgcactgc cactggcgaa
     2701 aatgcgggct ttgccgttaa gggtgaagtt gagatagtca atatcacgca ggatggtcct
     2761 gcctgaaacg ccagtctgtt gcgccagctc gccaggcgtg cgcggctgtt gctccagcag
     2821 atcgacaatc tttaactggc gttcgtttag cattaatcca cctgacggaa ctggctaaac
     2881 gcgttgactt tctccgcagt taaggtcgtc agaaaacccc gtatcagcgt gagcagagct
     2941 tcgtaatcgg cttttgaaat cataccgctg ttggcgtgca gataacgagt tggcagacac
     3001 agcgcgacaa ccggacgccc gccgcccatc acgttgtagc gcccgccatc cgtcgcaccg
     3061 gttttcatgg tggaaaattg cagcggtaaa tcgttatgtg cggcacagct ttttaacgct
     3121 gctaccagtt tctggttggg gaagtagcgc ttgtcaaaca gcatcagccc cggcccctgg
     3181 cccagtttca gcgggtattt aatgttatca atgcccggaa catcgcccgc tacggcggta
     3241 tccaacacga tcacgacgtc cggtttaatg tgttccgccg aggtttgcgc cccgcgtagc
     3301 ccaacttctt cttccacact gccaacgcca tacagcgtaa tttcgggatt attcaccgtc
     3361 tgcaatagtt cagccatcat tgcgcagccg atgcggttat ccaacgcctt gccgaccact
     3421 ttatcttcgc cccagcaggc aaaattggct tccgggctaa taaaattacc aatttccacg
     3481 ccgcgctttt ccacctcttc gcgactgttc gcgccaatat caatgaacat ctcatcaaat
     3541 gacagcggtt gttgcttttg cttttccgtt aacgcatgag gcgcgacgga accaatcaca
     3601 ccagggattt tcactccctt gtgtgtgcgt atggttaccc ggtggttgag catcgactga
     3661 ttccaccagc cgccaatggt ggtaaaacgc agaaaaccgc tctcgtcgat gtgggtgacc
     3721 ataaagccga cttcatccat atgtccgaca acggcaactt ttggaccttt attcccctta
     3781 cgggcaacaa agctgcccag accatcgaag gtgatttcat tcacgcaagg ttccagcgtg
     3841 tttatcagaa tgtcgcgaac ttcctgttca tcgccgctga cggcgctggc ttcgcacaac
     3901 tgttgcagta actcaatgtt catggacaag ctcctgttgc gcggctttcg ctttgcggcg
     3961 taaccacaat cctttcagga cgataatgaa tgaaatgttc agcgcgaggc caatcgccag
     4021 caccagataa aacgaatcta ccggcgacat caggccgatc agcgggtcaa agatacccaa
     4081 accaggtgcc agacgtttga ttccgaaagc gatcaccagc acgccggtaa tgccgcccgc
     4141 cagcgtattg gcggtaatca tcggcaacgg agctgccaga gcgtaaggta tagccggttc
     4201 ggttgcgact gtggcaccga ccacaatggc actactggca gcctcttttt cctgtcgggt
     4261 aaacagtttg ggcgcaatga acgtcgcaag acccgccgca acgggcggca tcaacgcgac
     4321 aacgccgacg atggcgtacc agtcataaat gtgtttttcc agcagtgaga agcagaagaa
     4381 ccaggcggtt ttattgatgg ggccgcccat atcgaacgcc agcatcgcac cgaccagaaa
     4441 cgctgccgca aacttcattg atggaggaat ggtattcagg aagtggagca gcccgcccat
     4501 gaggtcggac attaccggcc cgataacgta gtaagtgagc acgccgaaaa ccagcagagt
     4561 aacgaacggg atcagcattg aaccgagcag cggttgtaat gctttgccaa ggcgcacttt
     4621 gcggaaccag aagacgaaat agccaatcgc cagccccagc accaccgcgc cgaggaatcc
     4681 cgcacccgac tgggtgccga gcagcgcttt gtcgttcgcc agatagcaaa ccagaaacgc
     4741 cggagcgaaa gccggtttat cggcaatcga cgaggcaata taagcgccca tgatggggat
     4801 cataaaggtg aagccaaggt aaccgatgga ttcaactacc caggtaaacg acggcgcgcc
     4861 tttcgacata tcggtatacg gcaaaccgaa ctgcaccagc atgttggcaa gcgccaccag
     4921 gataccaccg ccgatgacaa acggcagcgc cgcagagacg cccgccatca ggtggctcat
     4981 cacgctgccg ctttgcactt cctgtttgcc aagcttcacg ccgctatctg cggcaaaaag
     5041 ctgcgagttt gtcggtaatt cgctgaaaat ctggtcgata tttttcaacg cctgggagat
     5101 ggcaatctca taaactttct tcccggcaaa ccgcgcgcga tcatcaccgc tcaggccacg
     5161 cccggtagcg agaatgacgt aatcggcagc ggcaatctct tcgctggata agcggttttc
     5221 aacgccactg gacccttgag tttccacttt tatggtatgg ccgagagaac gggctttctg
     5281 ttccagggct tccgccacca tgtaggtgtg agcgatcccg gcggggcagt tggtgatcgc
     5341 gacaatacgt aaggatgact ccatcgccac ttccttatga gctgagggtt tgatttaaca
     5401 aggcaagcac ctggtcggta tcgccctgtt gcagttgatg aataaaatcc tggtggataa
     5461 ttttgcgaca cagtgtgccg atgattttga cctgatcctc ttcgccgctt tgcggcacgc
     5521 cgaggcagat ccagcaattg acgtcttcgc catcgctggc tttccagtca atagcctgcg
     5581 ctttgcgggc gaataatacg aacggttgtt taacgcaggc gcttttaccg tgcggcacgg
     5641 cgacaccgga accaaatcca gtagagtgca ttttttcgcg cagcagcagg gtttgcagga
     5701 actggtgtga gtcggtgata aaaccgtttt gtaacgctat tgtcgccaac tgcttcagaa
     5761 cggaataagc gccattgccc tgaatattca gggcaataca gcttgcagta agagctgcca
     5821 ttatcggact ccatgaaaaa acatttcagt taattgtaac ggcggggcgg gtttgtgatg
     5881 agcgcggata tgtcgtgaag tcgtgacaat ataaagcaga gtg
//
LOCUS       DAJOSY010000174         5815 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJOSY010000174 DAJOSY010000000
VERSION     DAJOSY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.174"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..410)
                     /gene="hns"
                     /locus_tag="OHN27_004128"
     CDS             complement(<1..410)
                     /gene="hns"
                     /locus_tag="OHN27_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCQ2288473.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIK"
     gene            1015..1632
                     /gene="tdk"
                     /locus_tag="OHN27_004129"
     CDS             1015..1632
                     /gene="tdk"
                     /locus_tag="OHN27_004129"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCQ2288474.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(1934..4609)
                     /gene="adhE"
                     /locus_tag="OHN27_004130"
                     /gene_synonym="adhC"
     CDS             complement(1934..4609)
                     /gene="adhE"
                     /locus_tag="OHN27_004130"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCQ2288475.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAITTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETATKKEAAPAKAEKKAKKSA"
     gene            5086..5733
                     /gene="ychE"
                     /locus_tag="OHN27_004131"
     CDS             5086..5733
                     /gene="ychE"
                     /locus_tag="OHN27_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCQ2288476.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         1451 a         1305 c         1406 g         1653 t
ORIGIN      
        1 tgcttgatca ggaaatcgtc gagggattta ccttgctcat ccattgcttt tttgattaca
       61 gctggagtac ggccctggcc agtccaggtt ttagtttcgc cgttttcgtc aacgtagcta
      121 tattttgccg gacgctgagc acgtttagct ttggtgccag atttaacggc agcaaggcta
      181 ttcagcagtt cgttcgggtc aataccgtca gcgatcagca tttcgcgata ttgctgcagt
      241 ttacgagtgc gctcttcaac ttcagcagca gccgcgcttt cttcttcgcg acgttcgtta
      301 acaacaactt ctaatttttc cagcatttct tccagcgttt caagtgtaca ttctcttgcc
      361 tgcgcacgaa gagtacggat gttgttcaga attttaagtg cttcgctcat tgtagtaatc
      421 tcaaacttat attggggtgg tttgttgagg taataataga gccttaaatt cagttgtgca
      481 atagccagga atgtaaggaa ttcaaaattg ttctttattt tgtgccgcca ataaatatct
      541 tttcataaaa ttagccagaa aagacgcggc atatagccct atttacaccg atgatttcgc
      601 agcacgtgag gttaaaactt cctgattcat gtcacatttt atggggagat tatcgtaggc
      661 tgacgacctt tcagtcttct gtattagttg tgtttacgag aattccctat taagcgaatg
      721 atgaaaagta gaacagtcgc aataagagca tggacttagt attgcactat ctcctggagg
      781 tcaacagagg gctattactt gcgcaacagg ttaaagattg tgaatagtta ccagcagtca
      841 tttacccgct tataacaagc gaggcagttg taatgatagc tcagaaggat tatgcaaggc
      901 ttcgtaaggg agaacgcata tacccacttc tgtgcatact gttgagctga aaaactgacg
      961 aattatgata aactccagcc aactttattt catatcattg agggcctgtg gctgatggca
     1021 cagctatatt tctactattc cgcaatgaat gcgggtaagt ctacagcatt gttgcaatct
     1081 tcatacaatt accaggaacg cggcatgcgc actgtcgtat atacggcaga aattgatgat
     1141 cgctttggtg ccgggaaagt cagttcgcgt ataggtttgt catcgcctgc aaaattattt
     1201 aaccaaaatt catcattatt tgatgagatt cgtgcggaac atgaacagca ggcaattcat
     1261 tgcgtactgg ttgatgaatg ccagttttta accagacaac aagtatatga attatcggag
     1321 gttgtcgatc aactcgatat acccgtactt tgttatggtt tacgtaccga ttttcgaggt
     1381 gaattattta ttggcagcca atacttactg gcatggtccg acaaactggt tgaattaaaa
     1441 accatctgtt tttgtggccg taaagcaagc atggtgctgc gtcttgatca agcaggcaga
     1501 ccttataacg aaggtgagca ggtggtaatt ggtggtaatg aacgatacgt ttctgtatgc
     1561 cgtaaacact ataaagaggc gttacaagtc ggctcattaa cggctattca ggaaaggcat
     1621 cgccacgatt aataagaatt tctttactga cagggtgagc agggcacttt tatcctgtca
     1681 gttcgtttta cgcacttctt ccgggctata cacccttctc ggcagttttt taacgccgct
     1741 atacgcctca cagagcgctt atacaccgac attgacttgt gacctgtaaa gtacaatatc
     1801 cctgtgttta ggcattatac atcgtcgcaa atatgatgaa ggctaatgct gtcggtttat
     1861 ggaaaagttg ctttgggtaa acaaaaaaac ggccccagaa ggggccgttt atattgccag
     1921 acagcgctac tgattaagcg gattttttcg cttttttctc agctttagcc ggagcggctt
     1981 cttttttcgt tgcagtttca ccttctacat aatcacgacc gtagtaggta tccagcagga
     2041 tctgtttcag ctcggagatc agcgggtaac gcgggttagc gccggtgcac tggtcatcga
     2101 atgcatcttc agacagttta tccacgttcg ccaggaagtc tgcttcctga acgccagctt
     2161 cacggataga tttcggaata cccagttcag ctttcagcgt ttccagccat gccagcagtt
     2221 tctcgatctt agcagcagta cggtcgcccg gtgcgctcag acccaggtgg tcggcaattt
     2281 cagcataacg acggcgagcc tgcggacggt catactggct gaatgcagtc tgcttggtcg
     2341 ggttgtcgtt cgcattgtag cgaataacgt tacaaatcag cagggcgttt gccagaccgt
     2401 gcggaatatg gaactgggaa cccagtttgt gcgccattga gtgacataca cccaggaagg
     2461 cgttcgcaaa cgcgataccc gcgatagtcg ctgcactgtg aacacgttca cgcgctaccg
     2521 gatttttaga cccttcgtgg taggacgctg gcagatattc tttcagcagt ttcagtgcct
     2581 gcagagcctg accatcagag aactcagatg ccagtacaga aacataagct tccatggcgt
     2641 gagttactgc gtccagacca ccgaaagcac acagggactt cggcatgtcc ataaccaggt
     2701 tggcgtcgac aatcgccata tccggggtca gcgcatagtc cgccagcggg tatttctgac
     2761 cagtagcgtc gtcagttaca accgcaaacg gagtgacttc agaacctgta ccagaagtgg
     2821 tggtgatagc gatcattttc gctttcacgc ccattttcgg gaacttgtag atacgtttac
     2881 ggatatccat aaagcgcagc gccagctctt cgaagtgagt ttccggatgt tcgtacataa
     2941 cccacatgat cttcgcggcg tccatcgggg aaccaccacc cagcgcgata atcacgtctg
     3001 gtttgaagga gtttgccagt tctgcacctt tacgaacgat gctcagggtc gggtccgctt
     3061 ctacttcgaa gaagacttca gtttcaacgc ctgctgcttt cagtacggaa gtgatctgat
     3121 cagcataacc attgttgaac aggaagcggt cagtcacgat gagcgcacgt ttgtggccat
     3181 cagtaatcac ttcatccagc gcgattggca gggagccacg gcggaagtag atagatttcg
     3241 gaagtttgtg ccacaacatg ttttcagctc gcttagcaac ggttttcttg ttgatcaggt
     3301 gtttcggacc aacgttttca gagatggagt taccacccca ggaaccacaa cccagagtca
     3361 gggaaggtgc gagtttgaag ttatacaggt caccgatacc accctgagaa gccggggtgt
     3421 taatcaggat acgagccgtt ttcattttct gaccgaagta agaaacgcga gccggttggt
     3481 tatcctggtc agtgtacagg caagaggtat gaccgatacc acccatagca accagtttct
     3541 ctgctttttc taccgcgtct tcgaaatctt tagcgcggta cattgccaga gtcggggaca
     3601 gtttttcatg tgcgaacggt tcgctttcat caacaacggt cacttcaccg atcagaatct
     3661 tggtgttttc tggtacagag aagcctgcca gttcagcaat tttataggct ggctgaccta
     3721 cgatagccgc gttcagcgca ccgtttttca ggataacgtc ctgaacagct ttcagctctt
     3781 taccctgcaa cagatagccg ccgtgggttg cgaaacgttc acgtactgcg tcataaacag
     3841 agtcaacaac aacaacagac tgttcagaag cacagattac gccgttgtcg aaggttttgg
     3901 acatcagtac agatgcaact gcacgtttga tatcagcagt ttcatcaata acaactggag
     3961 tgttgcccgc gcctacaccg atagctggtt taccggagct gtatgcggct ttaaccatgc
     4021 ccggaccacc agtcgcgagg atcaggttga tgtctgggtg gtgcatcagt gcgttagaca
     4081 gttcaacaga aggttgatcg atccagccga tcagatcttt cggagcaccg gcagcgatag
     4141 cagcctgcag aacgatatca gccgctttgt tggtggcatc ttttgcacgc gggtgcgggg
     4201 agaagataat ggcgttacgg gtcttcagac tgatcagcga tttgaagata gcagttgaag
     4261 tcgggttagt ggtcggaacg ataccgcaaa taataccgat tggttcagcg atagtgatgg
     4321 taccaaaagt gtcgtcttca gacagaacac cacaggtttt ttcatcttta taggcgttgt
     4381 agatatattc agaagcaaag tggtttttga tcactttatc ttcgacgata cccatgccgg
     4441 attcggcaac ggccattttc gcgagtggga ttcgagcatc tgcagcagcc agagcggcgg
     4501 cgcggaagat tttgtctact tgctcttgag tgaaactggc atattcacgc tgggcttttt
     4561 ttacacgctc tacgagtgcg ttaagttcag cgacattagt aacagccata atgctctcct
     4621 gataatgtta aactttttta gtaaatcatc tgctcgaata cgagagtata gtcagtgcgg
     4681 tgatgatttg cttaacctat gaaaatcaaa agcttactcg cgctcacact cactgtgatt
     4741 tactaaaaga gtttaaacat tagagttatt atctctaatg cgtcacttcc aggtggcgta
     4801 agcaagatta ctcacttctg ggtactgatt acgtgatcca aatcaaattt ttgcaaagct
     4861 gacacctttc agcatcgctt ttcgccatta tagctaacag ttaataaatt gtagtatgat
     4921 ttggtggcta cattagcatg ttttgcacaa ctagataaca ataacgaatg atagcaattt
     4981 taagtagtta ggaggtgaaa aatgctgtca aaaggcgtat tgtcagcgcg tcttttcaac
     5041 cttatttatg gctaacatta tccggctttt gcttcggagc taaccgtgat tcagaccttt
     5101 tttgattttc ccgtttactt caaatttttc atcgggttat ttgcgctggt caacccggta
     5161 gggattattc ccgtctttat cagcatgacc agttatcaga cagcggcagc gcgaaacaaa
     5221 actaacctta cagccaacct gtctgtggcc attatcttgt ggatctcgct ttttctcggc
     5281 gacacgattc tgcaactttt tggtatatca attgattcgt tccgtatcgc cgggggcatc
     5341 ctggtggtga cgatagcgat gtcgatgatt agcggcaagc ttggcgagga taaacagaac
     5401 aagcaagaaa aatcagaaac cgcggtacgt gaaagcattg gtgtggtgcc attggcgttg
     5461 ccgttgatgg cggggccagg ggcgatcagt tctaccatcg tctggggtac gcgttatcac
     5521 agcattagct atctgtttgg tttctttgtg gctattgcat tgttcgcttt atgttgctgg
     5581 ggattgttcc gcatggcacc gtggctggta cgggttttac gccagaccgg catcaacgtg
     5641 attacgcgta ttatggggct attgctgatg gcattgggga ttgaatttat cgttactggt
     5701 attaagggga ttttccccgg cctgcttaat taattccttt caaatgaaac ggagctgcca
     5761 tgctccgttt acttcgtcat tatttttact ttgttcccgc gcagttatca aaagc
//
LOCUS       DAJOSY010000175         5769 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJOSY010000175 DAJOSY010000000
VERSION     DAJOSY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.175"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..376)
                     /locus_tag="OHN27_004132"
     CDS             complement(<1..376)
                     /locus_tag="OHN27_004132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288477.1"
                     /translation="MNNDVFQISPDEVTSELISAKERFLRGIQPYLCNGISNLADLHE
                     IQQHASKYFNTAVKLVQKVDLCKLDDNKTFAISLVGDCRAVLETYLEYYDMMVSNIKC
                     LEIDPNNIVLYSKDGLSNLQRII"
     gene            complement(373..636)
                     /locus_tag="OHN27_004133"
     CDS             complement(373..636)
                     /locus_tag="OHN27_004133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288478.1"
                     /translation="MSNYLEVKRKIGQYACVTRWGFPCAEREITLIQNDINSAIQSGK
                     VISRSMLQGIISRHVPNTHFLITDSVDNSDLNTALRMLAPKQK"
     gene            902..1096
                     /locus_tag="OHN27_004134"
     CDS             902..1096
                     /locus_tag="OHN27_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCQ2288479.1"
                     /translation="MLKQQDMTETAKVVFNELSIEPATVGEIAQNTYLSRERCQLILT
                     QLVMAGLADYQFGCYRRLQQ"
     gene            1246..2298
                     /locus_tag="OHN27_004135"
     CDS             1246..2298
                     /locus_tag="OHN27_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCQ2288480.1"
                     /translation="MKNTVKINSVDLINADCLHFIQSLPDDSIDLIVTDPPYFKVKPN
                     GWDNQWKGDEDYLKWLDHCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWVKPSGRWNGCNKESLRAYFPATERVLFAEHYQGPYRGKSDGYAAKERELKQHIMA
                     PLISYFRDARAELGITAKQIAEATGKKNMVSHWFGASQWQLPNEADYRKLQALFSRIA
                     AEKFQEQQLEQPHHQLVASYNSLNRKYSELLDEFKSLRRYFSVSVSVPYTDVWMHKPV
                     QFYPGKHPCEKPADMLRQIINASSRPGDLVADFFMGSGSTIKAAMALGRRALGVELES
                     ERFNQTVKEINELVGK"
     gene            2366..2581
                     /gene="essD"
                     /locus_tag="OHN27_004136"
     CDS             2366..2581
                     /gene="essD"
                     /locus_tag="OHN27_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCQ2288481.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            2581..3078
                     /gene="rrrD"
                     /locus_tag="OHN27_004137"
     CDS             2581..3078
                     /gene="rrrD"
                     /locus_tag="OHN27_004137"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCQ2288482.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCYGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            3075..3533
                     /locus_tag="OHN27_004138"
     CDS             3075..3533
                     /locus_tag="OHN27_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCQ2288483.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMQKRQRDVAELDARYTKELADANATIESLRADVSVGRKRLQVAATCAKS
                     TTGASGMGNGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            3950..4570
                     /locus_tag="OHN27_004139"
     CDS             3950..4570
                     /locus_tag="OHN27_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCQ2288484.1"
                     /translation="MKDQDVRFAVHHKLLKESHLDPDCLVVDEFSISLGASRADIAVI
                     NGVIHGYELKSEYDSLERLPLQIKHYSSVMDKVTLVVAEKHLEGALKLIPGWWGVKTV
                     SVGPKGAILIKHMRGEKLNRNHNSLMLAQLLWKDECIDVLERWGYSKGIKSKPRFELW
                     NIIAENIPIANLRLEVRTALKKRVGWKVKAWQAESAPTNKAVSRLT"
     gene            complement(4512..5579)
                     /locus_tag="OHN27_004140"
     CDS             complement(4512..5579)
                     /locus_tag="OHN27_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HCQ2288485.1"
                     /translation="MSQHQYYPQLKWKPAEYESLMLLDQTTLSGFTPIITIPDIDWDY
                     ENECYKKSLSSYLSDFGINLAASWKANRPVLLDVKYLDKHGSSRHHPLDMCIQDARVN
                     GKEIIPVVSPAYSTNYIHAVQRNLINGLAISITPQTWHQFTSLVNHLNIHPSLIDVII
                     DFGDIQNATDSLKQQALSMVNTLSGQAPWRNLILSSTAYPASQAGIPQHQVHHIPRHE
                     YDLWMYVVQNFSNGRTPSFSDYPTASSTITSVDPRFMSQYVSVRYSNDTSWIFVKGTA
                     VKGNGWGQTKNLCTTLVSSPEYHVFGSKFSWGDDYIYQRSLGANKSGGSKEWRKVAHT
                     HHITLVVRQLYWLAQTQPAKP"
BASE COUNT         1641 a         1186 c         1368 g         1574 t
ORIGIN      
        1 taatgattct ttggaggttt gatagaccat ctttgctata cagtacaata ttgtttggat
       61 caatttctag gcatttaatg tttgacacca tcatgtcgta gtattctaaa taagtttcca
      121 gaaccgctcg acagtctcca actaaggaaa tggcaaatgt cttattgtca tccagtttac
      181 ataaatctac tttttgaact aatttaacag cggtattaaa atattttgaa gcgtgctgct
      241 gaatttcatg taggtcagca aggtttgata taccattaca caagtatggc tgaataccac
      301 gtaaaaatct ttcctttgca gaaatcagct ccgacgttac ttcgtcgggg ctgatctgaa
      361 acacatcatt attcattttt gttttggcgc cagcattctt aatgcagtgt taagatcaga
      421 gttgtcgaca ctgtcggtga ttaagaaatg ggtattggga acatgacggc tgataatgcc
      481 ttggagcatg ctacgactta taactttacc actttgaatc gcggagttaa tgtcattttg
      541 tatcaaagtg atttcacgct cggcacaggg gaaaccccac cgagtaacac atgcatattg
      601 accaattttt ctcttaactt ccaagtagtt actcattgct gttctccttg tttgtaacca
      661 cgccgatagt atcaaaaagt aaaataataa aaaactaacg cgtacgcaaa aaaaattgta
      721 tcgtgttaag agtggttact tcgccacaca gcttaaaccc gccgtcgagc gggttttttg
      781 tacctgtaaa ccaagagcag tacggtaaac acgctggtgg tcgtgaatac tgacttttta
      841 tcttgctggc tttttagaca agagttattg gtatgtcatg ttaaccagaa gggaaaaaga
      901 catgctaaaa cagcaagata tgacagaaac ggcgaaagtt gtttttaatg aattaagcat
      961 cgaaccggca acagtcgggg agattgcaca aaacacatac ctttcacgcg aacgctgtca
     1021 gttaatactg acccagctgg ttatggcggg gctggcagat taccagttcg gctgttacag
     1081 acgccttcag caatgaaggg cttttaattt gtgaaaatgg gcggctggtg ggtgttggta
     1141 gcacttgcca gccattcgct catgcttact ggtcacaagc gaaccatggc ccactgcttt
     1201 agcgcaaaag cagagtgagc ctaccagagt tacgcttact gatccatgaa aaatactgta
     1261 aaaataaaca gtgttgattt aatcaacgct gattgcctgc attttattca gtccctgcct
     1321 gatgactcca ttgatctgat tgttaccgat ccaccgtact tcaaagtgaa gcccaacggc
     1381 tgggacaatc agtggaaagg ggacgaagat taccttaagt ggctggacca ctgtctggcc
     1441 cagttctggc gggtgttgaa acctgccgga agcctttacc tgttctgtgg acatcgcctg
     1501 gcatctgata tcgagatcat gatgcgtgaa cgtttcaatg tgcttaacca tatcatctgg
     1561 gtgaagccgt ccggacgttg gaatgggtgt aataaagaaa gtttgcgcgc atattttcct
     1621 gccacagagc gcgttctgtt tgctgaacat taccaggggc catatcgcgg caaaagtgac
     1681 ggctatgcgg caaaagaaag ggaactcaaa cagcacataa tggcaccgct gatatcgtat
     1741 ttcagggatg ctcgtgccga actgggtata acggcaaaac agattgccga agccacaggt
     1801 aagaaaaata tggtttccca ctggtttggt gccagtcagt ggcagttgcc gaatgaggct
     1861 gactaccgga agttacaggc actgttttcc cgtatagcgg cagagaagtt tcaggaacaa
     1921 caactggaac aaccacacca ccaactggtg gcatcttata attcactgaa tcgcaaatat
     1981 tctgaattgc tggatgagtt taaatctctc cggcgctatt tctccgtatc agtctccgtg
     2041 ccttataccg atgtctggat gcataaaccc gttcagttct acccgggtaa acatccgtgt
     2101 gagaaacctg cggatatgct ccggcaaata atcaatgcca gtagtcgacc tggtgatctg
     2161 gttgctgatt tttttatggg atccggttcc acaataaaag cagcaatggc gctggggcgt
     2221 cgggccttag gtgttgagct tgagtcagag cggtttaacc agacggtgaa agagataaac
     2281 gagctggtgg ggaaataatc tggtggccac gtcaggtggc ctttttattt ccattacaca
     2341 gcacccgcat ctgcgaggtg gggttatgaa atcaatggat aagttaacaa caggtgtcgc
     2401 ctatggcacc tctgcaggca gtgctggcta ctggttttta cagttgctcg ataaagtcac
     2461 gccatcacag tgggcggcaa taggtgtgct gggtagtctg gtatttggcc tgctgacgta
     2521 cctgacaaac ctttatttca agattaaaga agataagcgt aaggctgcga gaggtgaata
     2581 atgtcgccat cattacgcaa ggctgttgct gctgctattg gtggtggggc tgttgccata
     2641 gcgtctgtgc tcatcactgg tccgagtggt aacgatggtc tggaaggtgt cagttacata
     2701 ccatacaaag atattgttgg cgtatggact gtatgttacg gacacactgg aaaagacatc
     2761 atgctcggta aaacgtatac cgaagcagaa tgcaaagctc tcctgaataa agaccttgcc
     2821 acggtcgcca gacaaattaa cccgtacatc aaagtcgata taccggaaac aacgcgcggc
     2881 gctctttact cgttcgttta caacgtgggt gctggaaatt tcagaacatc gacgcttctt
     2941 cgcaaaataa accagggtga tattaaaggc gcatgtgatc agctacggcg ctggacatac
     3001 gctggcggta atcaatggaa aggactgatg actcgccgtg agattgagcg tgaagtctgt
     3061 ttgtggggga aacaatgagc agagtaaccg cgattatctc cgctctggtt atctgcatca
     3121 tcgtctgcct gtcattggct gttaatcatt accgtgataa cgcaatcgcc tacaaagagc
     3181 agcgcgataa caaggccagt gaactggaga aggcgaacgc caccatcgct gacatgcaga
     3241 agcgtcaacg tgatgtagca gaactcgacg ccagatacac aaaggagctt gctgatgcta
     3301 acgcgactat cgaaagtcta cgtgctgatg tttctgttgg gcgtaagcgc ctgcaagtcg
     3361 ccgccacctg tgcaaagtca acgaccggag ccagcggcat gggcaatgga gaaagcccag
     3421 gacttacagc agatgctgaa ctcaattatt accgtctccg aagtggaatc gacaagataa
     3481 ccgcgcaggt taactacttg caggagtaca tcaggacgca gtgcttaaaa taattttaat
     3541 ttcactgaaa tttaacaagt gactttcagg aaaatgcctc gcagatgcgg ggcatttttg
     3601 tacaggtatt tcaccgcgca ccgcagcgca ctcaaccacg tcgaaccaaa ccctttggaa
     3661 tgagcctttg agtagtcagt tagtgctggt gagccttgac gggctgatct cctatgcagc
     3721 aaaggttcat ctcaaagtaa ggcgaacgct atgacaaacc aagattctat agacctatct
     3781 gatcttcgtg gaatggtcag ttttccagac aaaaggtaat caccatagtc gtatggctat
     3841 gaatcttgtt gctgcagata agcattttgt gattgaagtg gtcaaatctc tcaaatgcag
     3901 taaaatgcag tgcgctataa ttcagtaacg gagggagtaa ggagaagtca tgaaagatca
     3961 agatgttagg ttcgcggtgc atcataagct tttgaaagaa tcgcatttag atcctgactg
     4021 ccttgtggtc gatgaatttt ccatatccct tggcgccagt agagcagaca ttgctgtaat
     4081 aaatggtgtt atacacgggt acgagctcaa aagtgaatat gactctttgg agcgtttgcc
     4141 tcttcaaatc aagcattatt cttctgtaat ggacaaggtt actcttgtcg tagctgagaa
     4201 acatcttgag ggagcattaa agttaatccc aggttggtgg ggcgttaaaa cggtttctgt
     4261 tgggccaaaa ggcgccattc ttataaagca catgcgtgga gaaaagctta atcgaaacca
     4321 taactcattg atgctcgctc aattgctttg gaaagatgaa tgtatcgacg tacttgaacg
     4381 atggggctat tccaaaggaa tcaaaagcaa gccccgattt gagttatgga atattattgc
     4441 ggaaaatatt ccaatagcga atctcaggct tgaagtcaga acagccttaa agaaacgcgt
     4501 aggctggaaa gttaaggctt ggcaggctga gtctgcgcca accaataaag ctgtctcacg
     4561 actaacgtaa tatggtgcgt atgtgcaact ttacgccatt ctttagagcc gccagatttg
     4621 ttagcgccta atgatctttg gtaaatgtaa tcatcccccc aactaaattt ggagccaaag
     4681 acgtgatact ctggcgaact aacaagggta gtacataagt ttttagtttg gccccatcca
     4741 tttcctttaa ctgcggtacc ttttacaaag atccatgagg tatcgttcga atatctcact
     4801 gagacatact gagacatgaa gcgtgggtct acgctcgtaa tggtagagct agcggtggga
     4861 taatcactaa aacttggcgt tcttccattg ctaaaattct gtactacata catccaaaga
     4921 tcgtattcat ggcgcggaat atgatgaact tgatgttgcg gtatccctgc ctgtgatgca
     4981 gggtatgcgg ttgaagataa aatcaagttt ctccacggag cttggcctga taatgtgttg
     5041 accatgctta atgcttgttg ttttaaacta tcagttgcgt tttgaatatc tccaaaatca
     5101 atgattacat caattaaact aggatgaata tttaagtggt taaccagact tgtgaattgg
     5161 tgccatgtct ggggggtgat agatatagcg agcccattga ttaagttgcg ttgaacagca
     5221 tgtatatagt ttgttgaata tgcgggagag acaacaggga taatttcctt accatttact
     5281 ctagcatctt ggatacacat atctagagga tgatggcggc tcgaaccatg tttatctaaa
     5341 tatttaacat ccagcaaaac aggacgattg gctttccagg atgccgcaag gttaataccg
     5401 aagtcagata agtaagaact caaactcttc ttgtagcatt cgttttcata atcccagtct
     5461 atgtctggaa tggtaatgat cggagtaaaa ccagagagcg tagtttgatc taaaagcatc
     5521 agagattcat attcagcagg cttccatttc agctgtgggt aatattgatg ttgactcatt
     5581 aaaactcctt aaaatcttta cttatcctaa gtatagtgtt atggcgtaac gccaattgtt
     5641 tttatacact tgaaaacatt gggaaaagtt gagctatatc taaatttaca aaattggatg
     5701 ttatagaaat cgaggtttca actaggcatg gaactgcaaa atttttaatg cctgcacgcg
     5761 aaagtcgtt
//
LOCUS       DAJOSY010000176         5648 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJOSY010000176 DAJOSY010000000
VERSION     DAJOSY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.176"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..240
                     /locus_tag="OHN27_004141"
     CDS             <1..240
                     /locus_tag="OHN27_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HCQ2288486.1"
                     /translation="FGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVD
                     IANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            267..1100
                     /gene="csgG"
                     /locus_tag="OHN27_004142"
     CDS             267..1100
                     /gene="csgG"
                     /locus_tag="OHN27_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCQ2288487.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(1164..1655)
                     /locus_tag="OHN27_004143"
     CDS             complement(1164..1655)
                     /locus_tag="OHN27_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCQ2288488.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1757..2311)
                     /gene="ycdY"
                     /locus_tag="OHN27_004144"
     CDS             complement(1757..2311)
                     /gene="ycdY"
                     /locus_tag="OHN27_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCQ2288489.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(2335..3072)
                     /gene="ycdX"
                     /locus_tag="OHN27_004145"
     CDS             complement(2335..3072)
                     /gene="ycdX"
                     /locus_tag="OHN27_004145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCQ2288490.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3127..4065)
                     /gene="ghrA"
                     /locus_tag="OHN27_004146"
     CDS             complement(3127..4065)
                     /gene="ghrA"
                     /locus_tag="OHN27_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCQ2288491.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGEKVCGQVDRARGY"
     gene            4299..4386
                     /locus_tag="OHN27_004147"
     tRNA            4299..4386
                     /locus_tag="OHN27_004147"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4333..4335,aa:Ser,seq:gga)
     gene            4431..4567
                     /locus_tag="OHN27_004148"
     ncRNA           4431..4567
                     /ncRNA_class="other"
                     /locus_tag="OHN27_004148"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(4941..>5648)
                     /locus_tag="OHN27_004149"
     CDS             complement(4941..>5648)
                     /locus_tag="OHN27_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288492.1"
                     /translation="QPLVAGFLSLIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLL
                     FRMIRTSYVKQVAISVAKKVKYKSLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQ
                     WLTFKSIIALLILCGVVLAINLFFLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAK
                     PLWLRLFILLVIEVMWITLVSVLATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHN
                     DFMMACDMYFRWGHFNK"
BASE COUNT         1554 a         1466 c         1324 g         1304 t
ORIGIN      
        1 tttggtattg aaacaccctc agcgttagat aactttactc aggccatcca gtcacaaatt
       61 ttaggtgggc tactgtcgaa tattaatacc ggtaaaccgg gccgcatggt gaccaacgat
      121 tatattgtcg atattgccaa ccgcgatggt caattgcagt tgaacgtgac agatcgtaaa
      181 accggacaaa cctcgaccat ccaggtttcg ggtttacaaa ataactcaac cgatttttaa
      241 gccccagctt cataaggaaa ataatcatgc agcgcttatt tcttttggtt gccgtcatgt
      301 tactgagcgg atgcttaacc gccccgccta aagaagccgc cagaccgaca ttaatgcctc
      361 gtgctcagag ctacaaagat ttgacccatc tgccagcgcc gacgggtaaa atctttgttt
      421 cggtatacaa cattcaggac gaaaccgggc aatttaaacc ctacccggca agtaacttct
      481 ccactgctgt tccgcaaagc gccacggcaa tgctggtcac ggcactgaaa gattctcgct
      541 ggtttatacc gctggagcgc cagggcttac aaaacctgct taacgagcgc aagattattc
      601 gtgcggcaca agaaaacggc acggttgcca ttaataaccg aatcccgctg caatctttaa
      661 cggcggcaaa tatcatggtt gaaggttcga ttatcggtta tgaaagcaac gtcaaatctg
      721 gcggggttgg ggcaagatat tttggcatcg gtgccgacac gcaataccag ctcgatcaga
      781 ttgccgtgaa cctgcgcgtc gtcaatgtga gtaccggcga gatcctttct tcggtgaaca
      841 ccagtaagac gatactttcc tatgaagttc aggccggggt tttccgcttt attgactacc
      901 agcgcttgct tgaaggggaa gtgggttaca cctcgaacga acctgttatg ctgtgcctga
      961 tgtcggctat cgaaacaggg gtcattttcc tgattaatga tggtatcgac cgtggtctgt
     1021 gggatttgca aaataaagca gaacggcaga atgacattct ggtgaaatac cgccatatgt
     1081 cggttccacc ggaatcctga cagataaaaa agccggaatt atcatccggc attttactgt
     1141 ttaagcgaaa gcgtgagtgc ccctcacgct ttatttttga tttcctgctc acggtgcgcc
     1201 gtctttgcac tacgcgccgc cagccacagg ccactgtttt tcattgcgta accaaatatc
     1261 agcccaagcg ccagcgaagg taacaccagt ttccagtcac cctgcccggc aaacgtcgcg
     1321 catgcgccta taaacgtccc cggaacaaat gaaagcagca gctgtttggc ctgaatacac
     1381 atcagaaacg cgacaatacc ggttatgaca taaccgagaa tttccagatg tggtgccagt
     1441 gcactaccgt aaataatgac catcgcccac accacgccac taagcagggt tgcagcggag
     1501 atcgccagcc ctttcagccc accttgcggg caggcaaagt aggccgtaca gcccaggaag
     1561 cccgcccagc ttagtaagcc cagggaaaca gccacccatc cccagatacc ggagagaatg
     1621 cctgttgtga ttgcaataga gagaagtatg ttcatgccgc gcatgatagc agaaaacgcg
     1681 gccatgaatg agatcgagca cacattttat gcaacaacaa attatccgtt gcagttatga
     1741 gtgacgtaga tcacacttat tcttcagaat cttcttccag ctcgtcccac attgcactaa
     1801 tggcatcgcg ggttagcggt gccatggtgc gccagaaagg cgtggttgca tgggcctcca
     1861 ctttgccaag gaacgcacca caccagggta acagatactc actgaacagt gtttccagtg
     1921 cttcgctctc atcttccgtt gactgatctt ccagccagga agccgcaagc agcaatgtgc
     1981 cgatgtgatc ggctggcgta tccgccagtg gcatccctcg ttcggaaaga aaagcgcgca
     2041 cttccgcttc cgttgcgtcc tcaacccatg cgctacgata tggcggcacg gcacattcat
     2101 cgccgataaa caacgcattg taatcggcag agacttgcgc catatcacag cttttctgta
     2161 aacgcgtcag taactcatcc tgctccagtg gccagttcgc agccagtttc ccctcacgaa
     2221 tcagggtaaa cagcggcacc agtaaaggat cttgcggttg gcggtaatag agcgaaccca
     2281 gcacacgaca gaggatagaa aactcgttca ttaatatatt tccattacta gtaattaaag
     2341 gtctgcaaat tccgcaatcg gtgccatacc gcgagattca aggaagttca gtaagcggcg
     2401 cggagaaaca ttcaaaatgc gctctggcgg aaaatctacc gcgtcgagga ttttaagaca
     2461 ctcttcaaat tcccccatgg taaacgcggt gtgagaatcc gagcctaatg ccacccaacc
     2521 tccagcatca cgtaccgctg cggctaccgc acgacagttg tcttcactgc ccttacgtga
     2581 gtgtaaaaat gaagaattat tgatttccag cgccacctga tgtttcgcgg ctgcttcagc
     2641 aacggctttc acatctattt catatttggg atttccggga tggcttatta tatgcacatt
     2701 gccgctggcg atagtggcga tcatcgcttg tgtattggtc gctttgtcat gtggtgcaaa
     2761 aaccggctca tgaaaaccgg caataatcag atccagcgag tcaaacattt taccgctgca
     2821 atcaatttca ccatcaacat ttttaatgtt agcttcgatg ccgcgcagga tccctacccc
     2881 atcaaccact cgcggccaga tacgcatgtt aatgaagtgc cagtgatgcg gagcatcttc
     2941 catatccggg ccatgatcgg tgatcgcaaa aagtttaatg cccttttgtt tggcctgggc
     3001 aatgtaatca ctcaatgtgc tatatgcatg tgtgctggca acggtatgca tatgaaggtc
     3061 gacgggatac ataactctct cctgtattca attcccgcca aggatagcag gaatcctgat
     3121 gctttattag tagccgcgtg cgcggtcgac ttgcccgcag accttctccc ctttttcgag
     3181 ctgggcaata gtgcgagaaa tgtactccac agcttcagcg ggacgggtaa tcgcggcgac
     3241 atgtggtgtt atcgtcacgc gtggatgttg ccagagcgga ctttcaggcg gtaagggttc
     3301 acgattaaaa acatccagca ttgcgccttt aactttcccg ctatccagcg ccgcgagcag
     3361 gtcatcttcc acaacatgaa caccacgcgc caggttgagg agatacgcgc catccggtaa
     3421 tttttcgagt aattgttgat taataatgcc gacggtttca ggggtattcg gtaacaaatt
     3481 aatcaatacc cgacattggc tcagaaatgc agacagttct tcccgtccgg caaagctttg
     3541 cacgccaggc cacgatttac gggttcgact ccagcaacgc agcggaaagc gccaggtttg
     3601 cagactctga gcaactttac tgcccagtac gcctgcgccc aaaatgccga tggtaaaatc
     3661 ttcccgatga tattcaggca gcggttgcca atgcgaacta ttttgctgga tgcgataatc
     3721 gtcaaaacgt cgaaaccaat gcagcacctg actgacagca tattcctgca tttgctcgcc
     3781 cataccggta tcttccaggc gaaaaagtgg aacagaaggg ttcagcattt cagggtgtgc
     3841 ctgtagcttg ctcaaaatag aatcaacacc ggccccgagt gcgaacaccg ctttaagatc
     3901 gcgccctgcc agcatttcaa caggaggatg ccagactaaa gcataatcag cagagtcatt
     3961 atctccgctt ttccatgctc tgactcttgc ctgaggaata gctttgcgca gtgcctcaat
     4021 ccaccattgg gtatcgaacg ttgggtgata aaagatgata tccatactga ctcccgaaaa
     4081 gcgttgtgcg aaatttattc gcacttatcg ttatgatcta caaaagccac cagcataacg
     4141 aatccgtggt cggtggcaaa aaaagcagat ttcgcttatt aaaactgcat attgattgaa
     4201 gtttgaataa acgcgcgatt ttttaaaaaa gtttgttgac ctcaggtcat gatttcccta
     4261 aattagcgcc cgttccagca agacaggaac gacaatttgg tgaggtgtcc gagtggctga
     4321 aggagcacgc ctggaaagtg tgtatacggc aacgtatcgg gggttcgaat ccccccctca
     4381 ccgccatatt taaagaagag ctcgtacgaa agtacgggct tttttttcgt atattgcaca
     4441 caccgggggg atgagaagcc ccgaccgggg ttcgacaact ggcgacagcc agttggacag
     4501 accgtgaacg cagtgagcgg gctgcccgca gggcgagcga agcgagtcaa tccccccctc
     4561 accgccatat ttaaagaaga gctcgtacga aagtacgggc ttttttttcg tgtattgcac
     4621 acaccggggg gatgagaagc cccgaccggg ggtcgacaac tggcgacagc cagttggaca
     4681 gaccgtgaac gcagtgagcg ggctgcccgc agggcgagcg aagcgagtca atccccccct
     4741 caccgccata tttaaagaag agctcgtacg aaagtacggg cttttttttc gtatattgca
     4801 cacaccgggt gcccccgccc acgtctgtta gggcgagggg aaatcgtgca catcaactgt
     4861 ctggttactc agtcaacaat caaaatgcgc aagagagtac agaacatata tgtattatct
     4921 ggtcacttcc tatcacttcc tcacttatta aaatgccccc aacggaaata catgtcacat
     4981 gccatcataa aatcgttatg ccagaggaat cgacaataga aaaaatagta aattaagcac
     5041 ggtacatagc agagtaaaaa ataggcttca aaccaaatcc gccattccac aagcgttgcc
     5101 aataccgaca ccagcgtaat ccacatcact tcaataacca gcaatatgaa taaccgaagc
     5161 caaagcggct tggcaaaaaa ggtcgacaac gcattttcac tggagaaaaa cagatcgatt
     5221 tcctgcaaaa aaagcctgcc cagaaaagcg taccgcttgg agaagcgcag aaaaaacaga
     5281 ttaatcgcca acactacgcc acaaagaatg agcaatgcaa ttatcgattt aaacgttaac
     5341 cactgcccct ctgtcacaaa agaatcactg ttacgcaacg ggctgatagt aagcaggtta
     5401 acaaccgttg agatgcacac cgaaagcagc acatagcgag tcagactttt atattttact
     5461 tttttcgcta ctgaaatagc gacctgcttg acataactgg tgcggatcat ccgaaacagc
     5521 agaaagatga ccaacggccc aagtaacgaa atcaggaaca gcgcatataa cgagggtaga
     5581 agctggtata gcccccaact cattgcactc aacatgatta acgacagaaa tcctgccacc
     5641 agaggttg
//
LOCUS       DAJOSY010000177         5647 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJOSY010000177 DAJOSY010000000
VERSION     DAJOSY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.177"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1744
                     /gene="kdpB"
                     /locus_tag="OHN27_004150"
     CDS             <1..1744
                     /gene="kdpB"
                     /locus_tag="OHN27_004150"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCQ2288493.1"
                     /translation="TAFARKLREPKYGAVADKVPADQLRKGDIVLVEAGDIIPCDGEV
                     IEGGASVDESAITGESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMI
                     AMVEGAQRRKTPNEIALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCL
                     IPTTIGGLLSAIGVAGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQAS
                     EFIPAQGVDEKTLADAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFT
                     AQSRMSGINIDNRMIRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEG
                     SRVLGVIALKDIVKGGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEA
                     TPEAKLALIRQYQAEGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLD
                     SNPTKLIEVVHIGKQMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRL
                     HSPDSAILSAVIFNALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIG
                     IKVNDLLLTVCGLV"
     gene            1753..2325
                     /gene="kdpC"
                     /locus_tag="OHN27_004151"
     CDS             1753..2325
                     /gene="kdpC"
                     /locus_tag="OHN27_004151"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCQ2288494.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLDNHITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVINIVELNLALDKLDE"
     gene            2318..5002
                     /gene="kdpD"
                     /locus_tag="OHN27_004152"
     CDS             2318..5002
                     /gene="kdpD"
                     /locus_tag="OHN27_004152"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCQ2288495.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRTQGLDIVVGVVETHGRKDTAAMLEELAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSSINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            4999..>5647
                     /gene="kdpE"
                     /locus_tag="OHN27_004153"
     CDS             4999..>5647
                     /gene="kdpE"
                     /locus_tag="OHN27_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCQ2288496.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITE"
BASE COUNT         1182 a         1514 c         1697 g         1254 t
ORIGIN      
        1 aaccgctttt gctcgcaagc tgcgcgaacc gaaatatggc gctgtggcag acaaagttcc
       61 tgccgatcaa ctgcgtaaag gtgacatcgt gctggtagaa gctggcgata ttatcccctg
      121 cgatggtgaa gtcattgaag gcggcgcatc ggtcgatgaa agcgccatca ccggggaatc
      181 ggcaccggtg atccgtgaat ccggcggcga ttttgcctcc gtcaccggcg gcacgcgtat
      241 tctttctgac tggctggtga ttgagtgtag cgttaacccc ggcgagacat ttctggatcg
      301 gatgatcgcg atggtggaag gcgcacagcg acgtaaaacg ccgaacgaga ttgccctgac
      361 catcctgctg attgccctga ctatcgtctt tttactggca accgccacgc tgtggccgtt
      421 ttccgcgtgg ggcggtaatg cagtcagcgt aacggtactg gtggcgctgc tggtctgtct
      481 gatcccaacc actattggcg gcctgttgtc agcgatcggc gtcgccggga tgagccggat
      541 gctgggcgcg aatgtgattg ccaccagcgg acgtgcagtt gaagcggcag gtgacgttga
      601 cgttctgcta ctggataaaa ccggcaccat cacactcggt aaccgtcagg cgtcggagtt
      661 tatccccgcg cagggcgtgg atgaaaaaac gctggctgac gccgcacaac tggcttcgct
      721 ggctgatgaa acgccggaag gccgcagtat tgtgatcctc gccaagcagc gttttaacct
      781 gcgcgagcgc gatgtgcagt cgctccatgc cacctttgta ccgtttactg cgcaaagccg
      841 gatgagcggg atcaacatcg acaaccgcat gatccgtaaa ggttctgtcg atgccattcg
      901 ccgccatgtt gaggctaacg gcggtcactt ccctgccgat gttgatcaaa aagtcgatca
      961 ggttgcgcgt cagggagcca cgccgctggt ggtggtggaa ggttctcgtg tgctgggcgt
     1021 tattgcgctg aaagatatcg tcaaaggcgg tattaaagag cgcttcgccc agctgcgcaa
     1081 aatgggcatt aaaacggtga tgattaccgg cgataaccgt ctgactgccg ccgccattgc
     1141 tgcggaagcg ggcgtcgatg attttctcgc cgaagcgaca ccggaggcca agctggcatt
     1201 gattcgtcag tatcaggcgg aaggccgttt ggtggcgatg accggcgacg gcaccaatga
     1261 tgctccggcg ctggcgcagg cagatgtcgc ggtagcgatg aactccggta ctcaggcggc
     1321 gaaagaagcg ggcaatatgg tggatctcga ctctaacccg accaagttga ttgaggtggt
     1381 gcatattggc aaacagatgc tgatgacccg tggctcgctg accaccttca gtattgccaa
     1441 cgatgtggcg aaatacttcg ccattattcc ggcggcgttc gcggcaacgt atccgcagtt
     1501 aaatgcgctg aacatcatgc gcctgcattc gcccgactcc gccattctca gtgcggtcat
     1561 tttcaacgcc ttgattatcg tctttttgat tcccctggcg ttaaaaggcg tgagttataa
     1621 accgcttacc gcttctgcca tgttgcgccg taacttatgg atttacggtc tgggtgggct
     1681 gctggtgccg tttattggta tcaaagtcaa tgatttactg ctgaccgttt gcggtctggt
     1741 gtgaggttta ccatgagtgg attacgtccg gcattatcga catttatctt tctgttattg
     1801 attactggcg gtgtttaccc gctgctgacc accgtactgg ggcaatggtg gtttccctgg
     1861 caggccaatg gttcgttgat tcgtgaaggt gatacggtgc gcggttcggc attaatcggg
     1921 cagaatttta ccggcaacgg ctattttcat ggtcgcccgt cggcaacggc agaaatgccc
     1981 tataatccac aggcttctgg cgggagcaat ctggcggtca gtaaccctga gctggataaa
     2041 ctaatagccg cacgcgttgc tgcattacgg gccgctaacc cggatgccag cgcgagcgtt
     2101 ccggttgaac tggtgacggc atcggcaagc gggctggaca atcatatcac cccgcaagcg
     2161 gcggcctggc aaatcccacg cgtggcgaaa gcgcgtaatc tcagcgttga acagctcacg
     2221 caactgatcg caaaatacag ccaacaaccg ctggtgaaat atatcggcca gccggttatc
     2281 aacattgttg aactcaatct ggcgctggat aaacttgatg aataacgaac ccttacgtcc
     2341 cgaccccgat cgtctgctgg aacaaactgc cgcgccgcat cgggggaagc tgaaagtttt
     2401 cttcggtgcc tgtgcaggcg tcgggaagac ctgggcgatg ctggcagaag cacagcgact
     2461 gcggacgcaa gggctggata ttgtggttgg cgtggtagaa acccacgggc ggaaagatac
     2521 cgccgccatg ctggaagagc tggctgttct gccgccaaaa cgcctggcgt accgtgggcg
     2581 gcatatcagc gagtttgatc tcgatgccgc cctcgcccgc cgtccggcgc tgatcttaat
     2641 ggacgaactg gcgcacagta atgcgcctgg ttctcgtcat cccaaacgct ggcaggatat
     2701 tgaagagctg ctggaagcag gcattgatgt ttttactacc gtcaacgttc aacatctgga
     2761 aagcctgaat gatgtggtca gcggcgtcac cggaattcag gtacgggaaa ccgtgcccga
     2821 tccttttttc gatgccgccg acgacgtggt gctggtggac ttgcccccgg acgatctgcg
     2881 ccagcggctg aaagaaggca aagtctatat tgccgggcag gcggagcgcg ccattgaaca
     2941 ttttttccgc aaaggcaatc tgatcgccct gcgcgaactg gcactgcgcc gtactgccga
     3001 tcgcgttgat gagcaaatgc gcgcctggcg ggggcatcct ggcgaagaga aagtctggca
     3061 cacgcgcgac gcgatccttt tatgcatcgg ccataacacc ggcagcgaaa aactggtccg
     3121 cgcggcggcg cggctggcgt cacggctggg gagcgtctgg cacgcggtgt atgttgaaac
     3181 ccctgccctg catcgcttac cggagaaaaa acgtcgtgca attctcagcg ccttacgtct
     3241 ggcgcaggag ctgggcgcgg agacggcaac actttctgat ccagcggaag agaaagcggt
     3301 agtgcgttat gcccgtgaac ataatctcgg caagattatt ctcggtcgcc cagcctcgcg
     3361 ccgctggtgg cgtcgggaaa cgtttgctga ccgactggcg cgcatcgccc ccgatctcga
     3421 tcaggtgctg gtcgcgcttg atgaaccacc cgcccgcacg attaacaacg cgccggatag
     3481 ccgctctttt aaagacaagt ggcgggtaca gattcaggga tgcgtggttg ccgccgcgtt
     3541 atgcgccgtt atcaccttaa ttgccatgca gtggctgatg gcgtttgatg ccgccaacct
     3601 ggtgatgctg tatctgcttg gcgtggtggt ggtggcgcta ttttatggac gctggccttc
     3661 ggtggttgct accgtcatta atgtggtgag tttcgatctc ttttttatcg ccccacgcgg
     3721 cacgctcgcc gtctctgatg tgcaatatct gctgaccttc gcggtgatgt taaccgtcgg
     3781 gctggtgatc gggaacctta ctgcgggtgt gcgttatcag gcgcgggtag cccgttaccg
     3841 cgagcaacgc acacggcact tatatgaaat gtcgaaagct ctggcggtgg gccgcagtcc
     3901 gcaggacatc gccgccacca gcgaacaatt tattgcctcc acgtttcatg cccgcagtca
     3961 ggtgttgttg cccgatgaca acggtaaatt gcagccgtta acacatccgc aaggaatgac
     4021 gccgtgggac gatgccatcg cgcagtggag ttttgataaa ggcctgcctg cgggtgcggg
     4081 caccgacacg ttacccggtg taccgtacca aattttgccg ttaaaaagcg gcgagaaaac
     4141 ctacggactg gtggtggtgg aaccggggaa tctgcgtcag ttgatgattc cggagcagca
     4201 gcgcctgctg gagacgttta cgctgttagt cgccaatgcg cttgagcggc tgacgctaac
     4261 cgccagcgaa gaacaggcgc ggatggcaag cgaacgtgaa cagatccgca acgccctgct
     4321 ggcggcgctt tcgcatgatt tacgcactcc gctcacggtg ttgttcggtc aggcagaaat
     4381 cttaacgctc gatctggcaa gcgaaggatc gccccacgcc cgccaggcca gcgagatccg
     4441 tcagcacgtg ctgaacacta cccgactggt gaataatcta ctggatatgg cgcgaattca
     4501 gtccggcggc tttaatttga agaaagagtg gttaacgctg gaagaagtgg tcggcagcgc
     4561 gctgcaaatg ctggaaccgg gtttatcgtc gtccatcaat ctttctctgc cagaaccgct
     4621 gaccttaatc cacgttgacg ggccactctt tgaacgggtg ctgattaatc tgctggagaa
     4681 cgcggtgaaa tatgcgggtg cgcaggccga aattggtatc gatgcccacg ttgagggcga
     4741 aaatctacaa ctggatgtct gggataacgg ccccggtctt ccgccaggcc aggagcagac
     4801 gatatttgat aagtttgctc gcgggaataa agagtcggca gtaccggggg tagggcttgg
     4861 actggcaatt tgtcgggcga tagtggatgt acacgggggc actattaccg cgttcaaccg
     4921 accggaaggt ggtgcctgtt ttcgtgttac acttccccag caaactgccc ctgaacttga
     4981 agaatttcat gaggatatgt gacaaacgtt ctgattgttg aagatgaaca ggctattcgt
     5041 cgctttctgc gcacggcgct ggagggcgac gggatgcgcg tctatgaggc cgaaacgctg
     5101 caacgcggct tgctggaagc ggctacccgt aagccagatt tgattattct cgatctcggc
     5161 ctgcccgatg gtgatgggat tgagtttatc cgcgacctgc gccagtggag tgcggtgccg
     5221 gtgattgtgc tttccgcacg cagcgaagag agcgacaaaa tcgccgcgct ggatgccgga
     5281 gcggatgatt atctgagtaa gccgtttggc attggcgaat tgcaggcccg tctgcgcgtc
     5341 gcattacgcc gccactctgc caccaccgca cccgatccgc tggtgaaatt ttccgatgtt
     5401 accgtcgatt tagccgcccg cgtgattcac cggggtgagg aagaggtgca tctcacacca
     5461 attgagttcc gcctgctggc ggtgctgctc aacaatgccg gaaaagtact cacccagcgc
     5521 cagctcctta accaggtgtg ggggccaaac gcggtcgaac acagtcacta tttgcgtatt
     5581 tatatgggac atctgcgaca aaaactggaa caggatcccg cccgcccacg ccatttcatt
     5641 actgaaa
//
LOCUS       DAJOSY010000178         5600 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJOSY010000178 DAJOSY010000000
VERSION     DAJOSY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.178"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            21..536
                     /gene="fimA"
                     /locus_tag="OHN27_004154"
     CDS             21..536
                     /gene="fimA"
                     /locus_tag="OHN27_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCQ2288497.1"
                     /translation="MAAALFAATGSYAAVVDGGTIHFEGELVNAACSVNTDSADQVVT
                     LGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAINDNLLAIASST
                     NTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGTGTSASAGQA
                     NADATFIMRYE"
     gene            756..1448
                     /gene="fimC"
                     /locus_tag="OHN27_004155"
     CDS             756..1448
                     /gene="fimC"
                     /locus_tag="OHN27_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCQ2288498.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGNLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1479..4088
                     /locus_tag="OHN27_004156"
     CDS             1479..4088
                     /locus_tag="OHN27_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCQ2288499.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4067..5107
                     /gene="fimH"
                     /locus_tag="OHN27_004157"
     CDS             4067..5107
                     /gene="fimH"
                     /locus_tag="OHN27_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCQ2288500.1"
                     /translation="MHTSWLIKEIINKIICRLLLAMACLCLANISWATVCANSTGVAE
                     DEHYDLSNIFNSTNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQET
                     IDNYKYMQLHDYLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFR
                     LKVIRPFINMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIV
                     EFDFGDIGASLFSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAM
                     VSDNQDLGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGP
                     FSALGYLRVDYQ"
     gene            5118..>5600
                     /gene="sfmF"
                     /locus_tag="OHN27_004158"
     CDS             5118..>5600
                     /gene="sfmF"
                     /locus_tag="OHN27_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HCQ2288501.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     K"
BASE COUNT         1457 a         1223 c         1356 g         1564 t
ORIGIN      
        1 taagatttat ttcgtctgcg ctggctgccg cactattcgc cgctacgggt agttatgctg
       61 ccgttgtaga tggcggtaca attcactttg aaggcgaact ggtgaatgct gcctgttcag
      121 tgaatactga ctcggcagac caggttgtca cactcggtca atatcgtacc gatattttca
      181 atgctgttgg taatacctct gcattaattc cattcaccat tcagttgaac gactgcgatc
      241 ctgttgttgc cgctaatgct gccgttgcat tttctggtca ggctgatgca atcaatgata
      301 atttattggc cattgcatcc agtaccaata caacaacagc aacgggtgtc ggtattgaaa
      361 tacttgataa tacatccgca attctcaaac ctgatgggaa tagcttctca accaaccaga
      421 acttgatccc cgggaccaac gttcttcatt tttctgcacg ttataaaggc accggtacaa
      481 gtgcatcagc agggcaagca aatgctgacg cgacttttat tatgagatat gaataatcaa
      541 aaccacgttg ttttgaatta tatatcacgt cttataacaa agtaatgtac cggttgtctg
      601 aagcggtatg gtggcaatgt aaatcgaaat catgttcact ttgtatcatg ccgctttatt
      661 aaatgaaaag ggaatgatgt gttgtaagaa accaaagcaa tcatttcttt atattcctta
      721 tttttgccgt caggaataca caaggcgtat taactatgat gactaaaata aagttattga
      781 tgctcattat attttattta atcatttcgg ccagcgccca tgctgccgga gggatcgcat
      841 taggtgccac gcgtattatt tatcccgctg atgctaaaca gactgcggta tggattagaa
      901 atagccatac caatgagcgc tttctggtca attcgtggat tgaaaacagc agcggtgtaa
      961 aagaaaagtc attcatcatt acaccgccac tgtttgttag tgaacccaaa agcgaaaata
     1021 ctttgcgtat tatttacacc ggtccaccgc tggcagcaga tcgtgagtct ctgttctgga
     1081 tgaatgttaa gacgatccct tcggtagata aaaatgcatt gaacggcagg aatgttttgc
     1141 aactggcgat tttatcgcgc atgaaattat ttctccgtcc aattcaatta caagaattac
     1201 ccgcagaagc gccggacaca ctcaagtttt cgcgatccgg taactatatc aatgttcata
     1261 atccatcacc tttttatgtc accctggtta acttacaagt gggcagccaa aagttgggga
     1321 atgctatggc tgcacccaga gttaattcac aaattccctt accctcagga gtgcagggaa
     1381 atctgaaatt tcagaccgtt aatgattatg gttcagtaac tccggtcaga gaagtgaact
     1441 taaactaacc gaatcatctg acaatatcag agctaattat gaaaataccc actactacgg
     1501 atattccgca gaggtatacc tggtgtctgg ccggaatttg ttattcatct cttgccattt
     1561 taccctcctt tttaagctat gcggaaagtt atttcaaccc ggcattttta ttagagaatg
     1621 gcacatccgt tgctgattta tcgcgctttg agagaggtaa tcatcaacct gcgggcgtgt
     1681 atcgggtgga tctctggcgt aatgatgagt tcattggttc gcaggatatc gtatttgaat
     1741 cgacaacaga aaatacaggt gataaatcag gtgggttaat gccctgtttt aaccaggtac
     1801 ttcttgaacg aattggcctt aatagcagtg catttcccga gttagcccag cagcaaaaca
     1861 ataaatgcat caatttactg aaagctgtac ctgatgccac aattaacttt gattttgcag
     1921 cgatgcgcct gaacatcact attcctcaga tagcgttgtt gagtagcgct cacggttaca
     1981 ttccgcctga agagtgggat gaaggtattc ctgctttact cctgaattat aatttcaccg
     2041 gtaacagagg taatggtaac gatagctatt tttttagtga gctcagcggg attaatattg
     2101 gcccgtggcg tttacgcaac aatggttcct ggaactattt tcgcggaaat ggatatcatt
     2161 cagaacagtg gaataatatt ggcacctggg tacagcgcgc cattattccg ctgaaaagtg
     2221 aactggtaat gggagacggc aatacaggaa gtgatatttt cgatggcgtt ggatttcgtg
     2281 gtgtacggct ttattcttct gataatatgt atcctgatag ccagcaaggg tttgccccaa
     2341 cggtacgtgg gattgcccgt acggcggccc agctaacgat tcggcaaaat ggttttatta
     2401 tctatcaaag ctatgtttcc cccggcgctt ttgaaattac agatttgcac ccgacatctt
     2461 caaatggcga tctggacgtc accatcgacg agcgcgatgg caatcagcag aattacacaa
     2521 ttccgtattc aacagtgcca attttacaac gcgaagggcg tttcaaattt gatctgacgg
     2581 cgggcgattt tcgtagcggt aatagtcagc aatcatcacc tttctttttt cagggcacgg
     2641 cactcggtgg tttaccacag gaatttactg cctacggcgg gacgcaatta tctgccaatt
     2701 acaccgcctt tttgttaggg ctggggcgca acctcgggaa ctggggcgca gtgtcgctgg
     2761 atgtaacgca tgcgcgcagt cagttagccg acgacagtcg tcatgagggg gattccattc
     2821 gcttcctcta tgcgaaatca atgaacacct tcggcaccaa ttttcagtta atgggttacc
     2881 gctattcgac acaaggtttt tatacccttg atgatgttgc gtatcgtcga atggaggggt
     2941 acgaatatga ttacgattac gacggtgagc atcgcgatga accgataatc gtgaattacc
     3001 acaatttacg ctttagccgt aaagaccgtt tgcagttaaa tatttcacaa tcacttaatg
     3061 actttggctc gctttatatc tctggtaccc atcaaaaata ctggaatact tcggattcag
     3121 atacgtggta tcaggtgggg tataccagca gctgggttgg catcagttat tcgctctcat
     3181 tttcgtggaa tgaatctgta gggatccccg ataacgaacg tattgtcgga cttaatgttt
     3241 cagtgccttt caatgttttg accaaacgtc gctacacccg ggaaaatgcg ctcgaccgcg
     3301 cttatgcctc ctttaacgcc aaccgtaaca gcaacgggca aaatagctgg ctggcaggtg
     3361 taggtgggac cttactggaa ggccacaacc tgagttatca cgtaagccag ggtgatacct
     3421 cgaataatgg gtacacgggc agcgccacgg caaactggca ggccgcttac ggtacgctgg
     3481 gggtcgggta taactacgac cgcgatcaac atgacgttaa ctggcagctg tctggcggtg
     3541 tggtcgggca tgaaaatggc ataacgctga gccagccttt aggggatacc aatgttttga
     3601 ttaaagcgcc tggcgcaggc ggtgtacgca ttgaaaatca aactggcatt ttaaccgact
     3661 ggcgcggcta tgcggtgatg ccgtatgcca cggtttatcg gtataaccgt atcgcgcttg
     3721 ataccaatac gatggggaat tccatcgatg ttgaaaaaaa tattagcagc gttgtgccga
     3781 cgcaaggcgc gttggttcgt gccaattttg atacccgcat aggcgtgcgg gcgctcatta
     3841 ccgttaccca gggcggaaaa ccggtgccgt ttggatcact ggtacgggaa aacagtaccg
     3901 gaataaccag tatggtgggt gatgacgggc aagtttattt aagtggtgcg ccattgtctg
     3961 gtgaattact ggttcagtgg ggagacggcg cgaactcacg ctgcattgcg cactatgtat
     4021 tgccgaagca aagcttacag caagccgtca ctgttatttc ggcagtttgc acacatcctg
     4081 gctcataaag gaaattatca ataagataat ctgcagatta ttattggcga tggcatgttt
     4141 gtgtctggca aacatatcct gggctactgt ttgtgcaaat agtactggcg tagcagaaga
     4201 tgaacactat gatctctcaa atatctttaa tagcaccaat aaccagccag ggcagattgt
     4261 tgttttaccg gaaaaatccg gctgggtagg tgtctcagca atttgtccac ccggtacgct
     4321 ggtgaattat acataccgta gttatgtcac caactttatt gttcaggaaa ctatcgataa
     4381 ttataaatat atgcaattac atgattatct attaggtgcg atgagtctgg ttgatagtgt
     4441 gatggatatt cagttccccc cgcaaaatta tattcggatg ggaacagatc ctaacgtttc
     4501 gcaaaacctt ccattcgggg tgatggattc tcgtttaata tttcgtttaa aggttattcg
     4561 tccctttatt aacatggtgg agatccccag acaggtgatg tttaccgtgt atgtgacatc
     4621 aacgccttac gatccgttgg ttacacctgt ttataccatt agttttggtg gccgggttga
     4681 agtgccgcaa aactgcgaat taaatgccgg gcagattgtt gaatttgatt ttggtgatat
     4741 cggcgcatcg ttatttagtg cggcagggcc ggggaatcga ccagctggcg tcatgccgca
     4801 aaccaagagc attgcggtca aatgtacgaa tgttgctgcg caggcttatt taacaatgcg
     4861 tctggaagcc agtgccgttt ctggtcaggc gatggtgtcg gacaatcagg atttaggttt
     4921 tattgtcgcc gatcagaacg atacgccgat tacgcctaac gatctcaata gcgttattcc
     4981 tttccgtctg gatgcagctg cggcagccaa tgtcacactt cgcgcctggc ctatcagtat
     5041 taccggtcaa aaaccgaccg aggggccgtt tagcgcgctg gggtatttac gcgtcgatta
     5101 tcaatgaggt acggagaatg agaagagtac tctttagctg tttctgcggg ttactgtgga
     5161 gttccattgg atgggcagct gaccctttag gaacgattaa tatcaatttg cacggtaacg
     5221 ttgttgattt ctcctgtacc gtaaacacag cggatattga taagacggta gatttaggca
     5281 gatggcctac gacacaatta ctgaacgctg gcgataccac ggcactcgtc ccttttagcc
     5341 tgcggctgga gggatgtcct ccgggttcag ttgcgatttt atttacggga acgccggcat
     5401 ccgataccaa cctgctggct ctggatgatc ccgcaatggc acaaaccgtc gccatcgaat
     5461 tacgtaatag cgatcgctcc cggctcgcac tgggggaggc gagcccgact gaggaagtag
     5521 atgcaaatgg caatgtcaca ctaaactttt ttgccaatta tcgagcgtta gccagcggtg
     5581 ttcggccagg tgtggcgaaa
//
LOCUS       DAJOSY010000179         5579 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJOSY010000179 DAJOSY010000000
VERSION     DAJOSY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.179"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(143..4723)
                     /gene="ypjA"
                     /locus_tag="OHN27_004159"
     CDS             complement(143..4723)
                     /gene="ypjA"
                     /locus_tag="OHN27_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HCQ2288502.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTTNVIGVVNDETV
                     DGNQKVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDIGSHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTTIESGNQDVYKGGISNGTTIKGGASRVEGGSANGILIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTHIYSGGTQIVDNTSTSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDTGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNM
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTKINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNLGQDSATKVNFGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPIPNPKPDPKPDPKPDP
                     NPKPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHN
                     NNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGITTLGAFMGYSHSHIG
                     FDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFKSNVAGKMSSGGAANGSYHSN
                     GLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMKSKSVDTRSIYRELGAT
                     LSYNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNSDGNFVNYLSGRRGIYQAGIKASF
                     SSTLSGHLGVGYSHSAGVESPWNAVAGVNWSF"
     gene            complement(4788..4907)
                     /locus_tag="OHN27_004160"
     CDS             complement(4788..4907)
                     /locus_tag="OHN27_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCQ2288503.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(5062..5280)
                     /locus_tag="OHN27_004161"
     CDS             complement(5062..5280)
                     /locus_tag="OHN27_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2288504.1"
                     /translation="MKKLVRTSSAGNALLVCKLNRLGRSMWHLVVLLEELCERGINFR
                     ALAQSIFAQQWGDECCKSKTICDLKVIV"
BASE COUNT         1306 a         1476 c         1214 g         1583 t
ORIGIN      
        1 aaggacaaca ccaacaaaaa caggaagtta gaagtctcag caaaacaccg accagacggt
       61 gaggagacat aaaaggatac gcaaaggagc cgcggctcct ggtaacatga aagcccacag
      121 atgtgggctt tttcgttgat ggtcagaacg accagttcac accagctacc gcgttccacg
      181 gggattccac accggcacta tggctatacc ccaccccaag atgcccgctt aacgtactgc
      241 tgaatgaggc tttaatacct gcctggtata ttccacgtct gcccgacaaa taattgacga
      301 aattaccgtc actattcact ttcacccggt tatcatcgac aaattctttg cgcacagccg
      361 ccttcagcca cggctcaact tccataccgt tccccagacg catgttgtaa ctcagcgttg
      421 cgcccagttc acgatatata ctgcgggtat cgactgattt cgatttcatg ccattggata
      481 aatgatattc ggggttatca gcggtgaacc ccgttaacga tgcatacggc gtcaggttcc
      541 agttaccatc ggtaaatcgc atcccggttt caatgtgacc gcccagcccg ttgctgtggt
      601 aactgccatt ggcggctcca ccgctgctca ttttacctgc tacgttactt ttaaaacggt
      661 tcagcttcac gacaccgtcc agatagaaac cactttcatg ttcccaactg gcatagccgc
      721 ccagagaata actgcccaca ctgccatgtc ctccgcgatc aaaaccgata tgtgaatggg
      781 aatagcccat aaaagcgcct agcgtggtaa ttccttcagg aatatcatta cggctgtcga
      841 tccccactgt cattccggtc agcgtctgct caaacccggc acccgcatcg gtggtgacat
      901 tattacgggt gttatacgtc gccccccaga cattattgtt gtgtggactc gctttcatta
      961 tgttcaaccg ctcgcgaata ctgtttagct cagcatcaaa taccaacggt aatgttgctg
     1021 ccatattgag tacggctgcc gtagaaggcg taatgcgttt ctccggaacg ggtgtcggcg
     1081 ttggatcggg agtagggtct ggttttggat ttgggtctgg ctttggatcg ggttttgggt
     1141 ctggctttgg atttgggatg gggtccgggt tgggtttgac atcattggtc aggttccagt
     1201 tgctgttgcc gtcacttttc aggacatact cataggtccc aagatcaacg aaaccgccgg
     1261 tattgcccag cgtaaacgaa gcatcccctc cccctgtttt caccagcgtc atcgcgtcgt
     1321 cagactgtgg gctgacgccg gtatcctgaa caaagatttt aaaattacca gtggcgttgt
     1381 tgttgacgac cagttgatcg ccccgggagc ctgcaacgtt ggtatgcagg tagaaattac
     1441 cgctaccgga aagttcattg gttgtcagcg tattgtagat accgttagtt gtggcaggcg
     1501 ctgctgtttg tgctgacaaa taaacatcac cgtcgttgag tagcaaactg tttactctat
     1561 attcgcagtt gctggtacct gcacaggaat tattgctgtt aagccagaca ctgccccggc
     1621 tggcagccgt caggtcggca agcgtggtat cgacgccatt tcccagagtc aatgtcgcgc
     1681 tatcggtaat ccggaccgcc ccttcgagtt taactggcgt aacattatcc cgtggcgtca
     1741 ttaacgacag gcttcctgtc gcgccactga ccgatgcatc cgccagcgta cctgcgtaga
     1801 cgattgccgt accgccagcg acctggagat cttctgcccg ggccagagcc tgaaactcat
     1861 cttttgaccg cccaagggta tattgcccgc caaagttaac ctttgtggcg gagtcctgac
     1921 ccaggttttg cattgcccca cccttgccaa ccgtggagtc gcgagcttcg gtacctgcta
     1981 ataccaataa attaccgcca ttttccagca acatattggt cgctaaattg ccggaaatgg
     2041 aaaaagtacc gtactggtga gtaccgctga tttcaatacc gttagccgtg ctcgtctgga
     2101 gagcggcacc gctgttctgg acgatatctg tcgctttgcc attatcgttg actgtcagcg
     2161 taccgccttc attgatcttt gtttttattg cctctccgtt agctgaaact gtttgtattc
     2221 cgccgtcgtt aattgttgtc tcattcgcca caccctcgac aatttgttca ccgccggtga
     2281 gcgtcgtgcc tgtcgcagtg gcttttgttt tgacgatctc ccgtccgccc atattgacct
     2341 gtgttttgtc agaagaggtg tctgactcca cggttaacac gccgccattt gccagcagga
     2401 tattgttcgc cgcaccctgc tcgatgctga acgcgacgcc atccgcgcgt gttcctgtga
     2461 cccgcgtcgc cctggtggtt gcaaccaaag cgccctggct actctgctgt atccccgttg
     2521 cgctgccttt ctcccgcaca tcgagtgtgc cgccgtcatt aagcaccgag ttttcagcca
     2581 gaccgccctc attaactacc tgtgaacccc cattaataat ggaaccttcc gctgtcccgt
     2641 ttgccataat ttgttgtagg ccagagacga tatcggtatt gattgcctta ccataattct
     2701 gaacggtttg cgtgccacca ttgatgtgtg ttttctctgt tgacccacca tcaacaattt
     2761 gttcaccact ttcgatattt gcttcagtgg ctaaaccata taccgtttgc ttgccacctt
     2821 tgatatttgc tttatcagaa gtggcactgg catatattgt ttgggtgcca gcactattga
     2881 gtacagtgcc aacatctttt ccataaacat ccattttgcc gttggcatta ataatcgtgt
     2941 caactgcccg ggaaccagtg acgactgtta atgagccagc gttttccagc actacatttt
     3001 tagcttctga attcctgatg tagaaagcat caccataact gttggttcct tcgataagtg
     3061 ttccggaggt cgtggaagca attaatgcgc cgccggattg ttgctcaaca tgcttagcct
     3121 caccaccgtc ctgaacctcc agaacgccgc cattattaag tctggtgcta tctgttttag
     3181 cctccttctg gacaatcagc ttaccgccag tatcaatggt agtatttttc gccgaggttt
     3241 tagccactac cgtcagttcg ccggtatttt ccagcacaac ataattagcc tcccctccgg
     3301 taatagtgaa gtgagagagc ttgttgtatc cttcgatatc agtccctgca cccgtgttgg
     3361 caactaaagc actgcccgtc tcctggttaa ccccatgtgc aataccgccg gtatagacaa
     3421 tcagcgagcc tccggcgcta atattgctgc caattgccgt accatctttc tcaacaacct
     3481 gccggctccc ggaggatata attgttgtgt cagctttccc gccgcttttg atattttgcg
     3541 ttccgctgtt gatattggtg cctgtggcta tgccataatt attaatattc tgtgtgccac
     3601 cattaattat ggtatttgtc gcgtttcctg caacatccat aaccccgcca ttatctattc
     3661 gggtcgcatc agctttagca ttggttaaaa ctgacattgt tcctttatct ttaataatcg
     3721 tcttgtttgc cgaaccatat gcgtttatgt ctaaatgacc accgttttcc agcaacacat
     3781 tgtctgccac gtgattgtgg atggagaatg caccttcact attcgtaccg ctcaccgtcg
     3841 taccgttagt gttagttttt aaaattgcac catcgtgctg ggtaacattt gttgccgtac
     3901 caccactaac atcaagcacg ccaccagaat aaacttcaat aacatccgag gtgctggtgt
     3961 tatcaacaat ttgcgtgcca ccagaataga tatgcgtatt ttttgccgtt gacttactat
     4021 tcagagactg agttccgcct tcaatcgtcg tgtccagcgc acggctctca tatactcttt
     4081 gctcaccgcc atttttaatg gttgttgttt ctactgtgct ctgttcaaca tactgtcgac
     4141 caccatttat ggttgtgttc gttgccagac ttccttgtac tacgtcctga gagccagact
     4201 tatttatcgt tgtaccatca gcatgccctt gaacttttac tatctggcta ccaccatcaa
     4261 tgagtattcc attcgcactc cctccctcta cgcgtgaagc accgccctta attgtcgttc
     4321 cattgctgat acccccttta taaacgtcct gattgccact ctcgattgtc gtacctgtgg
     4381 aaataccccc gtcatgaatt gactgtctgc caccgttaat ggttgtatta ttagcctgcc
     4441 ccacaaaatt gttatgactt cctatatctt gatatccacc agattcaata agacttccat
     4501 tagatacccc gccatgaaca ttctgctggc catggttgat aatatgagtg ttatttgttg
     4561 tacctcgttc atccactttt tggttgccat ctacagtctc atcgtttacc acaccaataa
     4621 cattagtagt gaaggcagcc atcccgggcg gggcatatat caaggcagat atcaataagg
     4681 aaagtactga gcggcgacaa taatagggac tggtcctgtt cataaatttc atcctctgaa
     4741 aagtgaatac tgagtagcgt ttaagcgacc ttagctttgc tgcaacatca gcccacaggc
     4801 accagaccag gggattcatc ctgaagagac agcgcaagtg tattgtgttc accgctcatc
     4861 aaagacatca tgatgaaatg atgatattcc gcataagaat gaggcatttt ttaaacgcag
     4921 tgcgctgaag tgtggttgga taaaaaagtc aatccattca ggaaatacgg gcgtattctt
     4981 ttctttcgac aatgaggccg ttggcaaaat aaaatgattt acataatcgt ttctgatgaa
     5041 tatcttctgc tcacataaaa atcacacaat aactttgaga tcgcagattg ttttactttt
     5101 acagcattcg tccccccatt gttgggcaaa tatagattgg gccagagcac gaaagttaat
     5161 accacgttcg cacagctcct ccaacagcac gacaagatgc cacatactgc gccccagtcg
     5221 gttcagttta cagactagca gtgcgttccc tgccgatgat gtcctgacca gttttttcag
     5281 tccggacctc ctgccacggt tttacgttga agtggtaacc ctgagcacgc agttcttcag
     5341 tcaggcgtgg tgcaccgtaa cgctgttatt gctgggtaag atcaaaaaac tttcaggcag
     5401 ctaaggaaag ttgaaccaga cattagatga aatatttcaa ccaattacag caccaattca
     5461 gtcactgcca gcccaccaaa taaatcaagg ggttacatga aaacgtagcc cctttttctt
     5521 tggtagtgac actaaaatgg atgtagtgtg aagaataatc ccgtttactc aatcaataa
//
LOCUS       DAJOSY010000180         5393 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJOSY010000180 DAJOSY010000000
VERSION     DAJOSY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.180"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1062
                     /locus_tag="OHN27_004162"
     CDS             <1..1062
                     /locus_tag="OHN27_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="similar to VirB4; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HCQ2288505.1"
                     /translation="CENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            1059..1445
                     /gene="trbI"
                     /locus_tag="OHN27_004163"
     CDS             1059..1445
                     /gene="trbI"
                     /locus_tag="OHN27_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCQ2288506.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEVQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            1442..2074
                     /gene="traW"
                     /locus_tag="OHN27_004164"
     CDS             1442..2074
                     /gene="traW"
                     /locus_tag="OHN27_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HCQ2288507.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            2071..3063
                     /locus_tag="OHN27_004165"
     CDS             2071..3063
                     /locus_tag="OHN27_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HCQ2288508.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            3089..3550
                     /locus_tag="OHN27_004166"
     CDS             3089..3550
                     /locus_tag="OHN27_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCQ2288509.1"
                     /translation="MYKQATELMLNFKDRILIKGEEDTGKSTLLTEIRISDSDSRYYN
                     FKTLNSAGYNRLCDENIDNFDFLNTPEKTLILDGVRLCEKKMTSKVIRLIKQARKYHK
                     RLVVVADSCESEFIELLFDGVIALSFNSDRERSCNVYTPSRHRNTDNIYAR"
     gene            3580..4032
                     /locus_tag="OHN27_004167"
     CDS             3580..4032
                     /locus_tag="OHN27_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288510.1"
                     /translation="MSNNIKYGSRILDMGVLLEMKALVNANGGVSRCIMECRQLACEV
                     RHSQQLLDKLSCIDDWLCRFSSIMYHRFDKEQIQWREDVDRDYPREKVYIFSPDNVSV
                     HNNDIKDSANNTSGITVSVTASKICAGQKEFDAFLKLAEKLRENPTGN"
     gene            4060..4698
                     /gene="trbC"
                     /locus_tag="OHN27_004168"
     CDS             4060..4698
                     /gene="trbC"
                     /locus_tag="OHN27_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HCQ2288511.1"
                     /translation="MNWKTFALFALTAGMHITVHASDNVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            4695..>5393
                     /locus_tag="OHN27_004169"
     CDS             4695..>5393
                     /locus_tag="OHN27_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HCQ2288512.1"
                     /translation="MKRILPLILALVAGMALADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQAGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSW"
BASE COUNT         1360 a         1294 c         1533 g         1206 t
ORIGIN      
        1 tgtgagaaca tggggggcgt atatctggac ggtgaaaccc tgcgctttaa cccgtttgcg
       61 aacatcaccg atattgacca gtcagcggag cgtgtccgcg accagctgtc ggtgatggcc
      121 agccccaacg gtaacctgga tgaagtgcat gaaggtctgc tgctgcaggc agtcagggca
      181 tcatggctgg ccaaagagaa cagggcacgt attgatgacg tggtggattt cctgaaaaac
      241 gccagtgaca gcgagcagta tgccgaatca ccgaccatcc gcagccgtct ggacgaaatg
      301 attgtgctgc ttgaccagta cactgctaac ggcacttatg gccagtattt caactccgat
      361 gagccgtccc tgcgggatga tgcgaaaatg gtggtgctgg agctgggcgg actggaagac
      421 cgtccatccc tgctggttgc ggtgatgttc agccttatca tttacattga gaaccggatg
      481 taccgcacgc cgcgtaacct caagaaactg aacgtcattg atgaaggctg gcgtctgctg
      541 gacttcaaaa atcacaaggt cggtgaattt attgagaaag gctaccgtac cgcccgccgt
      601 cataccggtg cctatatcac catcacacag aacatcgtcg actttgactc tgacaaggcc
      661 tccagtgcgg cccgtgcagc atggggtaac tcctcctaca aaattatcct caaacagagt
      721 gcgaaggagt tcgcgaaata caaccagctg tatccggacc agttcctgcc actgcagcgt
      781 gacatgattg gtaagttcgg tgcggccaaa gatcagtggt tcagttcctt cctgctgcag
      841 gtggaaaacc attcctcctg gcaccgcctg tttgtggacc cgttaagccg cgccatgtac
      901 agctctgacg gcccggattt tgagtttgtg cagcagaaac gtaaagaggg gctgagtatt
      961 catgaggcgg tgtggcagct ggcgtggaag aagtcagggc cggagatggc ttcgctggaa
     1021 gcctggctgg aagaacatga gaaatacagg agtgttgcat gaccacaacg caaaaaacga
     1081 ctgacgttac agcccgtcga cgcagccact ggtggtggac ggtgccggga tgcctggcaa
     1141 tggtgttact gaacgcagcc atcagttacg gcattgtcag actgaacgca ccggtgactg
     1201 ccgccttcaa catgaaacag accgtggatg cgttttttga cagcgccagt cagaaacaac
     1261 tgtcagaagt acagtcaaaa gccctttcag cgcgttttaa cacggcactg gaagccagcc
     1321 tgcaggcatg gcagcagaaa caccacgcgg tcattctggt gtcgcctgcc gtggtacagg
     1381 gggctccgga tatcacccgg gaaatccagc aggatatcgc ccggcgaatg agggcggagc
     1441 catgagatgc cgggggctga ttgccctgct gatatggggg cagagtgtgg ccgccgccga
     1501 tcttggtacc tggggcgatc tgtggccggt aaaggagccg gacatgctga cggtgatcat
     1561 gcagcgcctg acggcactgg agcagtccgg tgagatgggc cggaaaatgg atgcctttaa
     1621 ggagcgggtg atccgcaaca gtctgcggcc ccctgccgtg cccggtatcg gacgcacgga
     1681 gaaatacggc agccggttgt ttgacccgtc cgtcagactg gctgcggata tccgggataa
     1741 cgaagggcgg gtgtttgccc gtcagggtga agtgatgaat ccgctgcagt atgttccttt
     1801 taaccagacg ctgtatttca tcaatggcga tgatccggcg caggtggcct ggatgaaacg
     1861 ccagacgccg cccacactgg agagcaaaat tatcctcgtg cagggcagta tcccggagat
     1921 gcagaagtct ctggacagcc gtgtttactt tgaccagaac ggtgtgctct gccagcgcct
     1981 cggcattgat caggtcccgg cacgggtgag cgccgttccc ggcgatcgct tcctgaaggt
     2041 ggaatttatt ccggcagagg agggcagaaa atgaagcgaa gcctgtggct gctgatgtta
     2101 ttccttctcg ccggtcatgt ccctgcagcg tctgcggatt ctgcctgtga ggggcgtttt
     2161 gtaaacccga tcacggatat ctgctggagc tgtattttcc cgctctcgct gggcagtatc
     2221 aaagtcagtc agggtaaggt ccccgacacg gcgaacccgt cgatgcccat tcagatttgt
     2281 ccggcaccgc cgccgctgtt caggcgtatc gggctggcca ttggttactg ggagccgatg
     2341 gcgctgacgg acgtcacccg atcaccggga tgcatggtta acctgggctt cagcctgccg
     2401 gcttttggta aaacggcaca gggaacggcg aaaaaggatg agaagcaggt gaacggggcg
     2461 ttctatcacg ttcactggta caaatacccg ctgacgtact ggcttaacat catcacatcg
     2521 ctgggctgtc tggaaggtgg tgacctggat atcgcttatc tttctgaaat cgaccccacc
     2581 tggacggaca gcagcctgac caccattctc aatccggaag ctgtcatctt tgccaatccg
     2641 atagcacagg gggcctgtgc agcagatgcc attgccagcg cctttaatat gcctctcgat
     2701 gttctgttct ggtgtgccgg ttcgcagggc agtatgtacc cgttcaatgg ctgggtgagt
     2761 aatgagtcca gtccgttgca gtcctccctg ctggtcagtg aacgcatggc gttcaagctg
     2821 caccgtcagg gcatgattat ggaaaccatc gggaaaaata acgccgtctg taatgaatat
     2881 ccgtccccaa tcctgcccaa agaacgctgg cgttaccaga tggtgaatat gtacccggac
     2941 agcggacagt gccatccgtt cgggcgcagt gtgacccgct gggaaaccgg gaaaaatccg
     3001 cccaatacaa agaaaaactt cggctacctg atgtggcgta aacgtaactg tgtcttcctg
     3061 taaaactact aattctgaag gattttttat gtataaacag gcaacagaat taatgctgaa
     3121 ctttaaagac cgcattctga ttaaggggga agaagatacc ggcaagtcaa ctctcctgac
     3181 tgaaataaga atcagtgata gtgattcccg gtattacaac tttaaaactc ttaactctgc
     3241 ggggtacaac cggctttgtg atgaaaatat tgataatttt gattttctga atacacctga
     3301 aaagacgtta atccttgatg gagtcaggct gtgtgaaaag aaaatgacat caaaagttat
     3361 caggttaatt aaacaggcca gaaaatatca taaacgcctg gttgtggttg cagactcatg
     3421 tgagtctgag tttatcgaac tgttgtttga tggtgttatt gcattgtcct tcaattctga
     3481 cagagaaagg agttgcaatg tttatacgcc atcaaggcat cgtaatacag ataatattta
     3541 tgctaggtaa atgtgtaaac aaaaaaagag gagggtgtaa tgagtaataa tataaaatat
     3601 ggctccagaa tacttgatat gggtgttctg ctggagatga aagcgcttgt gaatgcaaat
     3661 ggtggtgtgt cacggtgcat tatggagtgt cggcagcttg cctgtgaggt ccgtcattcg
     3721 caacaattac tggataagct gtcatgtatt gacgactggt tgtgccggtt ttcctcaata
     3781 atgtatcacc gttttgataa agaacagatt cagtggcggg aggatgtgga cagggattat
     3841 cccagagaaa aagtttatat tttcagtccc gataatgtct ccgtccataa taatgatatt
     3901 aaagactctg ccaataacac atcaggaata accgtgtcgg ttacagccag taaaatatgc
     3961 gcaggtcaga aagagtttga tgctttttta aaactggcag aaaaattgag agaaaaccct
     4021 acgggtaatt aaattgtaac aatgccatca ggatatatca tgaactggaa aacatttgcg
     4081 ctttttgccc tgactgcagg aatgcatatc actgttcatg cctcagacaa tgtgaacact
     4141 cctgaaaacc gccagttcct gaagcagcag gaaaatttaa gcagacaact gcgtgaaaaa
     4201 cctgaccatc agctgaaggc ctgggcggag aaacaggtgc tggagaaccc ccttcagcgt
     4261 tcagataacc atttcctgga tgagctggtt cgtaaacagc aggcttcgca ggacgggaaa
     4321 ccccggcagg gggccctgta ttttgtgtcg ttttccattc ccgaagaggg gctgaaacgg
     4381 atgctgggcg aaacccggca ctacggtata cccgccacac tgcggggcat ggtgaacaat
     4441 gacctgaaga ccaccgctga agccgtgctg tccctggtga aagacggcgc gactgatggt
     4501 gttcagatcg acccgacgct gttttcacag tacggcattc gcagtgtacc ggcactggtg
     4561 gtgttctgca gtcagggata cgacatcatc cggggaaacc tgcgggtcgg ccaggcgctg
     4621 gagaaagtgg ccgccacggg tgactgccgg caggtggctc atgatttact ggcagggaaa
     4681 ggagattccg ggaaatgaaa cgtattttac ctctgatact ggctctggtt gccggcatgg
     4741 cactggctga cagcaacagt gattaccggg ccggctctga ttttgctcac cagatcaaag
     4801 gacagggaag cagcagtatt cagggcttta agccacagga gagtatcccc ggctataacg
     4861 cgaatccgga cgagacaaaa tactacggtg gcgtgactgc cggcggggat ggcggcctga
     4921 aaaatgacgg caccacggaa tgggcgaccg gtgaaaccgg aaaaaccatc acagagtcat
     4981 tcatgaacaa gccgaaagac attctttcac cggatgcacc gtttatccag gccggcaggg
     5041 atgtggtgaa ccgggctgac agcattgtgg gaaacaccgg gcagcagtgc agtgcgcagg
     5101 agattagccg gagtgaatac acgaattaca cctgtgagcg ggatttacag gtggagcagt
     5161 actgcacacg aactgcccgt atggagctgc aggggagtac cacatgggaa acccggacgc
     5221 tggagtatga aatgagtcag ctacctgcac gtgaagtgaa tggtcagtat gttgtctcca
     5281 ttacctctcc cgttaccggt gaaattgtcg atgcgcatta cagctggagt cgtacttacc
     5341 tgcagaagag tgtacctatg acaattacag tactgggaac cccactgagc tgg
//
LOCUS       DAJOSY010000181         5390 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJOSY010000181 DAJOSY010000000
VERSION     DAJOSY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.181"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..573)
                     /locus_tag="OHN27_004170"
     CDS             complement(<1..573)
                     /locus_tag="OHN27_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCQ2288513.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRD"
     gene            complement(583..2727)
                     /gene="nrdE"
                     /locus_tag="OHN27_004171"
     CDS             complement(583..2727)
                     /gene="nrdE"
                     /locus_tag="OHN27_004171"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCQ2288514.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYTRTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(2700..3110)
                     /gene="nrdI"
                     /locus_tag="OHN27_004172"
     CDS             complement(2700..3110)
                     /gene="nrdI"
                     /locus_tag="OHN27_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCQ2288515.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3107..3352)
                     /gene="nrdH"
                     /locus_tag="OHN27_004173"
     CDS             complement(3107..3352)
                     /gene="nrdH"
                     /locus_tag="OHN27_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCQ2288516.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(3600..3929)
                     /locus_tag="OHN27_004174"
     CDS             complement(3600..3929)
                     /locus_tag="OHN27_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCQ2288517.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4081..4425
                     /locus_tag="OHN27_004175"
     CDS             4081..4425
                     /locus_tag="OHN27_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCQ2288518.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(4462..4911)
                     /gene="alaE"
                     /locus_tag="OHN27_004176"
     CDS             complement(4462..4911)
                     /gene="alaE"
                     /locus_tag="OHN27_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCQ2288519.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1365 a         1317 c         1345 g         1363 t
ORIGIN      
        1 atcgcggata atcagacgga tcaggtccgc ggtattggtc agctttccgc ggctggaaaa
       61 atacatcggc agccagaaac cggaatagaa caaaaaagat tcaagaaaca cactggcgat
      121 tttctttttc agcggatcat caccgcgata atgttgctga ataatctgag cttttcgctg
      181 caacggtgcg ttttcttcac tccaggcgta ggcggcatcg acatctttgg tctggcatag
      241 cgtcgagaaa atcgaactgt aagagcgggc atgaaccgct tccataaagc tgatattcga
      301 taataccgct tcttcatgag gcgtgagtgc atcgggcatc agagaaggcg cgccgataac
      361 attttgcagc gtgtcgagca gcgtcaggcc agtaaaaacg cgcatcgtca gttgttgttc
      421 tacgacagtt aatgtctgcc aggcaggaat atcgttcgac agcggcacct tttctggtag
      481 ccagaaattg ctggtcaggc gattccacac ctccagatct ttatcgtcag atatcttgtt
      541 ccagttgatg gcgctgatac gtgagagttt catagatatt ccttaaagtg cacaggagac
      601 gcagccttca atttcagtgc cttccagcgc catctgacgc aggcggatgt aatagagcgt
      661 tttgataccc ttgcgccagg cgtaaatctg cgctttgttg atatcgcgag tggtggcggt
      721 atcggggaaa aaaagcgtca gcgacagccc ctgatcgaca tggcgagtcg cttccgcgta
      781 ggtgtcgatg atcttttctg cgccaatttc gtaagcgtcc tgatacagcg ccagattctc
      841 gttagtcata aacggggcag ggtagtaaac gcgtcctgtt ttgccctctt tgcgtatctc
      901 tactttcgcc acaatcggat gaatactcga cgtagcatgg ttgatataag agatagaacc
      961 ggttggcggc accgcctgaa gattctggtt gtatatgccg tagcgcatca cgtcgtcgcg
     1021 cagctgcgcc cacatctcac gggtaggtaa cgtaataccg ctacgggtaa acagttcgcc
     1081 aactttcgcc gttttcggct gccagttccc ttgcagatat tggctaaaat attcaccact
     1141 ggcatagcgt gactgtttga acccggcgaa ggtttcaccg cgttcgcgtg ccagcaacat
     1201 cgaggtacgc agtgcatgcc aggtgatggc atagaaatag agattggtga aatccagtgc
     1261 ttccggcgaa ccataagcga tgccttctcg cgccagatag ccgtgtaaat tcatctgccc
     1321 cagtccgatg gcgtgcgagg cggcatttcc ggcttcgatg gacggcacgc tgcggatatg
     1381 actcatatct gatactgccg ttaaaccgcg cacggcagtc tctaccgtgc gggcaaaatc
     1441 gggggaatcc atggtgtgcg caatattcaa cgaacctaaa ttgcaggaaa tatcatggcc
     1501 tgtgcgggta tagtcgagat tctcgtcata ctctgaggcg ctgttaacct gcaaaatttc
     1561 tgagcagaga ttactcatat ttatgcgccc ggcgataggg ttagcacggt ttaccgtgtc
     1621 ttcatacatg atgtagggat agccggactc aaactggatt tctgccagtc gctggaagaa
     1681 atcacgggcg ttgaggtatt ttttgcgaat gcgttcatcg gcaaccagtt cgtcatagtg
     1741 ttggctgatg gccacatcgg caaacggctt gccataaact cgctctacgt cataaggcga
     1801 aaacagcgcc atctgcgcat tctcttttgc cagatggaaa gtgatatccg ggatcaccac
     1861 gccaagcgac agtgttttaa tgcggatttt ttcgtcggca ttttcccgtt tcgtgtcgag
     1921 aaaacgcaga atatcgggat gatgagcatg taaatagact gcaccagccc cctgacgagc
     1981 gccgagttgg ttggcatagg aaaatgcgtc ttccagcatt ttcatcaccg gaattacgcc
     2041 agaagattga ttttcaatac gtttaattgg cgcgcccgct tctcgcagat tcgacagcaa
     2101 aaatgctacg ccgccgccgc gtttcgacag ctgcagtgcg gaatttaccg cccgaccaat
     2161 cgactccata ttgtcttcaa tacgcagcaa aaaacaggaa accagttcgc cgcgctgctg
     2221 cttaccgcag ttgaggaatg ttggcgtggc tggctgaaag cgtcctgaca gcatttcatc
     2281 ggtcagttgc aacgccagcg tctcatcgcc ttgtgccagc gtcagcgcca ccatcgttac
     2341 tcgatcggca aaatcttcca gataacgttt accgtcgaat gtcttcaacg tatagctggt
     2401 gtaaaacttc catgccccga ggaatgtctg gaaacgaaaa ccgctggtgt gtgcgtgggc
     2461 aaacagcgta atgacaaagt cgcgagagta gcgattaaga acgctttcat catagtaacc
     2521 ttcgttgacc agccagttca ggcgctgctg ctggctactg aaggtcacac tgttcggacg
     2581 cacatgcgtc gcaataaagg cgtcaacggc ctggcggtct ttatcgaact gaatgcgacc
     2641 tgcgctatcg tagaggttaa gcatcgcatt cagcgcgtgg taatccatcg tttcctgcgt
     2701 caggcattct gcggttgtcg ttgccaaaat tcggttactc ctttacgaac gttttcgata
     2761 tcgctttgcg tacccatgag ttcaaaacgg tacagccacg gcacgccgca tttccgggca
     2821 atcacatctc cggcgcggcc atacgcctca ccaaagttgc gattaccaga agcaataacg
     2881 ccgcgaagca acgcccggtt gtgctcgtcg tttaaaaagc gaattacctg tcgtggcacc
     2941 gcgccagccg taccgccgcc gccgtaagag ggcacgatca ggatgtaagg ctcgtctacc
     3001 tgaatccgtt cccgctcatt gagcgggatg cgcaccgcgg gcagacctaa acgttcgata
     3061 aaacgctgcg tgttttcgga gctgctggag aagtagacga gctggctcat gcactggccg
     3121 cgtgtggcgc tggatgcaga cggttaatca tgtccggacg gaaaccagac cagctaagat
     3181 cgccagcaat cactaccggc aactgacgaa agccctgagc acgcaacgct tctgccgctt
     3241 caggaacgcg atcgacatta atcatttcaa aatcaaagcc ccggttttcc atcgcccgtt
     3301 tggtggcgtg gcactgaacg caatcgttac gagtgtaaat agtaatgcgc atgattcgta
     3361 tttccgttta aaatgaagat acggcgcgat gatacgcgtc gggttgtctc tctgttgata
     3421 cagagatact agatgtagtt gaaaaaagat tcaaccacac aatatatagc aaatagtggt
     3481 cgaaattacc ctggatatga gcgtgacggg gtagggggat ttttgtgatt caccaggcaa
     3541 aaagaaaccc cgaagacagg cttcggggtc aaagacgcgt atttattatc atttttgcac
     3601 tacgatttgc gcatgcttaa cagtgcgccg ataaaaatac ctaccgcagc tgctgtaccc
     3661 acgctacacc agggtctttc acgaacaaaa gaatccgcgc agccaacggc atcgcgcgcg
     3721 gcttgctgga cgcgagtacg accatgcatt cgcgccctgg tttctttcag caatgcctga
     3781 gctttactgc gtgcggcttc agcttcccct ttggcgtcgc tgccccagga cttcaataca
     3841 gattcgaggc tgtcagctaa ttgattgaca tcattctgga tatcctgcac gccatcatca
     3901 acgtcgttgc ggttcggtcg gttaaacata tgatccccca ttagaattcg atgtaatttt
     3961 cagtgtagac cataattttc ttaactgagg ttcatcgcgg ctttatggac ttttctgaaa
     4021 gcgttgtgaa tgcgcaatca ggtaaggtag gcccgcggtt gataataacg aggatcaaat
     4081 atgtatttac gaccagacga ggtggcgcgc gtacttgaaa aagtcggttt tactgtcgat
     4141 gtggtaaccc agaaagcgta tggttaccgc cgtggggaaa attatgtcta tgttaatcgc
     4201 gaagcgcgaa tgggacgtac cgctctggta attcatccaa cattaaaaga acgcagttcg
     4261 acactggctg aacccgcttc cgatattaaa acttgcgatc attaccagca atttccgctc
     4321 tatttagcag gcgagcgaca cgagcattac ggtatcccgc atggctttag ttcgcgtgtt
     4381 gcgcttgaac gttatttgaa tggtttattt ggcgaagcca gttaaagacg cgactggcga
     4441 tgccagtcgc gaaaagaaga gtcaggcttt tacctgctgg taacggctga ctttaaacag
     4501 tcggcggcaa taatcgagga agtagccata aaccgccccc atcaacatcg aaacaacgat
     4561 gtttgaactg accgccgcca taatctgatg ccagtctgcg cccaccacta acaagatcgc
     4621 cacatacacc ggtgactgga acgtcacata agccaggata tccgccagat tttttatcca
     4681 gcccgacggg ctaactttgc gtgccgcgcg cataaacaga tcacggtaca taccgtatgg
     4741 ccatgcaatt aagatgttca ccggaatcgc taccaatctg gaataaaaag actgttcgaa
     4801 gctcattccg gagaggaaaa cttcaataca catgttcacg acagaacagt aaacaaccat
     4861 cgcgaacgta tctgcaactg catgacgcaa gcgtgactgc ggtgagaaca tgctaatgct
     4921 ccttaataaa agcgtaatgg gatgttaatg gagatgaaaa cagtggatct cattgccatc
     4981 tgcattttcg catagcgtat atcgctggct atgactaatc aattagtgaa aaatttttat
     5041 ttagtcagtt tttgtagata aaatattccg taacggctcg ttttttgtac attgtggatt
     5101 tttacatgcc gatatgattt attttcaatt aaatcaatta gatatgacct ttcctttaat
     5161 ttcactaatg aaagtttggg ggtgaggtaa cgctataagc gtagagaatt cggtgtatgt
     5221 cgtgaccgtg tcatttacag tattttttat gccagaaaat gtacattaac agcagaaagc
     5281 accagtgagg ggtgttttct tttattctgt tatattgcat ttttattcac ttcggttttg
     5341 tcaggcttgc ggaattagcg agcagagagc gccgttctgt tcaccaataa
//
LOCUS       DAJOSY010000182         5382 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJOSY010000182 DAJOSY010000000
VERSION     DAJOSY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.182"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(108..1391)
                     /gene="aroA"
                     /locus_tag="OHN27_004177"
     CDS             complement(108..1391)
                     /gene="aroA"
                     /locus_tag="OHN27_004177"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCQ2288520.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1462..2550)
                     /gene="serC"
                     /locus_tag="OHN27_004178"
     CDS             complement(1462..2550)
                     /gene="serC"
                     /locus_tag="OHN27_004178"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCQ2288521.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGADVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2749..3441)
                     /locus_tag="OHN27_004179"
     CDS             complement(2749..3441)
                     /locus_tag="OHN27_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCQ2288522.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3571..5331
                     /locus_tag="OHN27_004180"
     CDS             3571..5331
                     /locus_tag="OHN27_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCQ2288523.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1390 a         1397 c         1271 g         1324 t
ORIGIN      
        1 cgacgctacc attaatcaaa atgactcaca aggtccgaaa aaaaacgccg acaatgtgcc
       61 gacgttttga taaagaaaga tttggctatt tattgcccgt tgttcattca ggctgcctgg
      121 ctaatccgcg ccagctgctc gaaataatcc ggaaatgttt tggccgtgca tttgggatca
      181 agaatcgtca ctggtgtatc tgacaacgcc accagcgaga aacacatcgc catccggtga
      241 tcattgtatg tcgcgatctc ggcaaagttc agtttttccg gaggagtgat acgaatgtaa
      301 tcgtgcccct cttccacttc cgcgccgact ttacgcagtt ctgttgccat cgcaaacagg
      361 cgatcggtct ctttaacacg ccagttatag atattgcgca gcgtggtggt gccttttgca
      421 aataacgccg ccgtggcaat ggtcatcgcc gcatcaggaa tatggttcat atccatatca
      481 atagcgttca gttcaccacg cgtgcaggaa atataatcat cgccccagca aatggtcgcg
      541 cccatttttt ccagcacatc agcaaagcga atatcaccct gcatactgtt acgtccaata
      601 ccggtcactt ttacagtgcc gcctttgatt gctgctgctg ccagaaagta agaagccgaa
      661 gatgcatcgc cttcgaccaa ataagtaccc ggagactgat aagactgccc gccttttacg
      721 acaaattgtt gatagtgctg attttcaatt tcaacaccaa acgtcttcat cagattgagt
      781 gtgatgtcga tataaggttt agaaaccaga tcgcctttaa tacgaatcac cgtatcttcc
      841 ggcgcaagag gcgcagtcat taacagtgcg gtgaggaatt ggctggaaac ggagccatca
      901 acgtcaacgt tgccgccagt aaagccgccc tgtaaacgca acggcggata attttcttgt
      961 tccaggtaag tgatcttcgc cccgcccagg cgcagcgcat ccaccagatg accaatcggg
     1021 cgttctttca tacgcggctc accggtcagc acaatatcat tgctacccag acaaagagct
     1081 gccgccagcg gacgcattgc cgttccggcg ttaccgagga acaactccag ggcaccttct
     1141 gcgtgtaatg gaccgccgtt accgataatt tcgcaacgcg tacgatcggc tgaaagcgta
     1201 tagcttaccc ctaacgctgt taatgcattc agcatatggc gcacgtcatc gctatccagc
     1261 agattggtta atactgtttt gccgtgtgct aatgccgcca gcaataaagc gcggttagaa
     1321 acgctcttgg aaccgggcag attaatagtg ccatcgacac gagcgatggg ttgtaacgtc
     1381 agggattcca tgaactcaac tctctacaac agaaataaaa accccacagg ctggctgtgg
     1441 ggattaagca aaatttcggc attaaccgtg acggcgttcg aactcaacca tgaagtctgt
     1501 cagcgcttta acgccttcca gcggcatggc gttataaata gaagcgcgca ttccgccgac
     1561 cacacggtga cctttcagtg catgaaggcc agcagcaaaa gactcttcaa ggaacaattt
     1621 gtcaagcgca ctgtccgcca actggaacgg cacgttcatc cgcgaacggt tagctttcgc
     1681 cacgtcattg cggtagaaat cgctgttatc aatcacccca tatagcagtt ctgctttttg
     1741 ctgattgatt ttatccattt cagctacacc gccgttcgct ttcagccatt taaagaccag
     1801 accagataga taccaggcaa atgtcggcgg cgtgttaaac atggagccgt tatcgttgag
     1861 gatggaataa tccagaatcg acggacacgc gatattcgct ttgcccagca aatcttcacg
     1921 aacgatgacg attgtcaggc cagccgggcc gatatttttc tgcgcgccag cgtaaattac
     1981 accataacgg ctgacgtcaa tcggacggga aagaatggtt gaagagaagt cagcggcgac
     2041 caccacatct gcgccgaagt ctggcgtttc gtcgatggcg ataccatcga tggtttcatt
     2101 cgggcaataa tgcatataag cagcattatc agagagttgc cattcacgca ttggcttaac
     2161 cgcgcgcaga ccatcaacag tcactttggc gtcaaagaca ttaggcgtgc agtatttttt
     2221 cgcttcttta atggcacttg ccgcccagta accggcatca acataatctg cggtggtttt
     2281 atcaccgaga atattcagcg gtaccgcagc aaactgaccg cgaccaccgc catggcagaa
     2341 taataccttg tagttggagg ggacattaag aagatcgcga aaatccttct cggcttcctc
     2401 tgcaacctga atgaactctt tgccacggtg actcacttcc atcaccgacg taccaagacc
     2461 gttccagtcg cgcagttcct gttgagcctg tttaagcacc tctgccggta gcattgccgg
     2521 accagaacta aaattgaaga tttgagccat ttcccctcac cacgttgcgt tgctattaac
     2581 ccgcggtcaa tcgattgcga ccacaggtaa tacattattc ctgtggctat cggttttatc
     2641 attcagtgac acacatcgca atgagtaaaa ccgttgaggg caaaaatgtg gcttgcatca
     2701 cacaaaagaa tctctcggat tattagcaaa aagccgatgt ttttactgtc aggtcacccg
     2761 ttttgcccga cttgccttcg tccattctgg atttgcacag cgcggacata attgtttttc
     2821 ccccgctttc atatgaatga tttcactaca acgaatacag gcatagtccg ccgactccgg
     2881 caccactttg taacgctgcg tacaatcact gggtaaaata agtaaacccg gtttcacctt
     2941 gtcatcttca aagaaataga cactgatttg cccgttggtt tcgagaatcg ccagacgtac
     3001 ctgccccagc tgctccacgc cacgcaatcg tagctccata aagaactcaa attccgtcat
     3061 gttggagtta ttgagtttcg accaggccag ctcgccatct tcaataatga caactggctt
     3121 gccttccaga agatcttcca gtttttcact gtgcgccatc aaccacatta ccaggcggta
     3181 taacaacgcc agagtaataa agacgataag taccgggacc atcggcacat catcataaaa
     3241 cgccacatct cccgccgctg atcccagcgt cagaatgatt aaaacttcaa acagcgacat
     3301 ctgccgcaca ccgcgtcttc cggtcatttt gaggaacaaa aagaccagta caaaggtata
     3361 aagactacgt agtgcaactt cgccaaggaa atcaaaaggc actttatcaa atgccatacg
     3421 gtggagatcg aacgccttca tttttatgct cttaacgtgg tttatccgct aaaagcatag
     3481 tcaacagttc atcggcatgg cggcgatgat agcgcctttg agataaaacc cgtattattg
     3541 cgcgctttcc gtacgactaa agtgattttc atgacgcaaa catttatccc cggcaaagat
     3601 gccgctctgg aagattccat cgctcgcttc cagcaaaaac tttcagacct cggctttcag
     3661 attgaagagg cctcctggct gaatcccgtg cctaacgtct ggtctgtaca tattcgcgac
     3721 aaagagtgcg cactgtgttt taccaacggt aaaggcgcaa ccaagaaagc ggcgctggct
     3781 tctgcactcg gtgaatattt cgagcgtctc tcaaccaact acttttttgc ggacttctgg
     3841 ctgggcgaaa ccatcgccaa cggtccgttc gtgcattatc ccaacgaaaa atggttccca
     3901 ctgaccgaaa atgacgatgt gccagaaggg ctgctcgatg accgtctgcg cgcgttttat
     3961 gatccggaga atgaactgac cggcagcatg ctgattgacc tacaatccgg taacgaagat
     4021 cgtggtattt gcggcctgcc gtttacgcgt cagtccgaca atcagaccgt ttatattccg
     4081 atgaatatca ttggtaacct gtacgtctcc aacggtatgt ccgcaggtaa tacccgcaac
     4141 gaagcacgcg ttcagggatt gtctgaagtt ttcgaacgct acgtgaaaaa ccgcattatt
     4201 gctgaaagca tcagcctgcc agagatcccg gcagacgtgc tggcgcgtta cccagcagta
     4261 gttgaagcca tcgaaacgct ggaagcggag ggtttcccaa tcttcgctta tgacggttcg
     4321 cttggcggcc agtatccggt gatttgcgtg gtactgttca atcctgctaa cggtacctgc
     4381 tttgcctctt tcggtgcgca tcctgatttt ggcgtagcac tggaacgtac cgtgaccgag
     4441 ctgctgcaag gtcgtggcct gaaagatttg gatgtgttta ctccgccaac cttcgatgat
     4501 gaagaagtcg ctgaacatac caacctcgaa acgcacttta tcgattccag cggtttaatc
     4561 tcctgggacc tgttcaagca ggatgccgat tatccgtttg tggactggaa tttctccggc
     4621 accacggaag aagagttcgc cacactgatg gctatcttca acaaagaaga taaagaagtt
     4681 tatattgccg attacgagca tctgggcgtt tatgcttgcc gtattatcgt gcctggcatg
     4741 tccgatattt atccggctga agatctgtgg ctcgcgaata acagtatggg cagccattta
     4801 cgtgaaacga ttctttcgct accaggcagc gagtgggaaa aagaagatta cctgaacctc
     4861 atcgagcaac tggatgaaga aggttttgat gactttaccc gcgtgcgtga gctgttgggt
     4921 ctggcgaccg ggtcggataa cggttggtac accctgcgta tcggtgaatt aaaagccatg
     4981 ctggcgctgg ctggtggcga tctggaacag gctctggtct ggaccgaatg gacgatggag
     5041 tttaactcat cagtatttag tccggaacgc gccaactatt atcgctgcct gcaaacgttg
     5101 ttattactgg cacaggaaga agatcgccag ccgctgcaat atctgaatgc gtttgttcgc
     5161 atgtacggcg cagatgccgt agaagccgcc agtgcggcaa tgagcggcga agcggcgttt
     5221 tacggcctgc aaccggtaga tagcgatctg cacgcgtttg ctgcacatca gtcgttgttg
     5281 aaggcctacg aaaagctgca gcgcgccaaa gcagcattct gggcaaaata aaatcaaata
     5341 gcctacgcaa tgtaggctta atgattagtc tgagttatat ta
//
LOCUS       DAJOSY010000183         5319 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJOSY010000183 DAJOSY010000000
VERSION     DAJOSY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.183"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(116..1024)
                     /gene="galU"
                     /locus_tag="OHN27_004181"
     CDS             complement(116..1024)
                     /gene="galU"
                     /locus_tag="OHN27_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCQ2288524.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1226..2239)
                     /gene="rssB"
                     /locus_tag="OHN27_004182"
     CDS             complement(1226..2239)
                     /gene="rssB"
                     /locus_tag="OHN27_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCQ2288525.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLALDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2331..3236)
                     /gene="rssA"
                     /locus_tag="OHN27_004183"
     CDS             complement(2331..3236)
                     /gene="rssA"
                     /locus_tag="OHN27_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCQ2288526.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIIAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            3349..3807
                     /locus_tag="OHN27_004184"
     CDS             3349..3807
                     /locus_tag="OHN27_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCQ2288527.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCTFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3857..4699
                     /gene="purU"
                     /locus_tag="OHN27_004185"
     CDS             3857..4699
                     /gene="purU"
                     /locus_tag="OHN27_004185"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCQ2288528.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4859..4943
                     /locus_tag="OHN27_004186"
     tRNA            4859..4943
                     /locus_tag="OHN27_004186"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4893..4895,aa:Tyr,seq:gta)
     gene            4987..5118
                     /locus_tag="OHN27_004187"
     ncRNA           4987..5118
                     /ncRNA_class="other"
                     /locus_tag="OHN27_004187"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            5153..5237
                     /locus_tag="OHN27_004188"
     tRNA            5153..5237
                     /locus_tag="OHN27_004188"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5187..5189,aa:Tyr,seq:gta)
BASE COUNT         1392 a         1352 c         1204 g         1371 t
ORIGIN      
        1 cccgccatcg gcgggatttt ttattgtccg gtttaagaca atttaataag aataagctat
       61 aaaaaaacgg cgtcgattgc tcaacgccgt ttcgtggata acaccgatac ggatgttact
      121 tcttaatgcc catctcttct tcaagccagg ctttaaattc cgtgccaagg gtgttatgac
      181 gaataccgta ttcaacgaag gcctgcatgt aacctaattt attaccgcag tcatggctct
      241 tccctttcat atgataggct tcaaccgttt ctttttcgat cagcatatca attgcgtcgg
      301 tgagctgaat ttcatcacca gctcccggag gggtttttgc cagcaacggc caaatatccg
      361 cactaagtac gtaacgaccc acaatagcga gattagacgg cgcaacatcc gcttttggtt
      421 tttcaaccac gccaaccatc ggtacgcttt cacccggcgc taattcaacg cctttgcaat
      481 ccacaacgcc atatgcggtc acatcagcaa ccggttcaac catgatctgg ctatgacccg
      541 tttcatcaaa gcggcggatc atctctgcca ggttatcctg tgacaaatcg gattcatatt
      601 catccagaat aacgtcaggc aaaataacag ccacaggttc atcacccact accgggtgag
      661 cacacaatac cgcgtgtccc aggcctttcg ccagcccctg acgaacttgc ataatagtca
      721 cgtgcggcgg acaaatagac tgcacttcat caagcagttg acgttttaca cgtttttcca
      781 gcattgcttc cagttcaaaa ctggtatcaa agtggttttc aatagagttt ttagatgagt
      841 gtgtaaccag cacaatttca gtaatgccag ccgcaataca ttcattcacg acgtattgaa
      901 ttaatggctt atcgacaagt ggcagcatct ctttcgggat ggctttcgtc gccggcaaca
      961 tcctggttcc taatcccgca acggggataa cggctttttt gactttcgta ttaatggcag
     1021 ccatttaaat tctcctggac tgttcgtgtt ttgaacgtgt tcatatttct gtatcgcatc
     1081 ccagtatatc agcaccccga aggggctaag ttctgaaaag gaggcgagtt agcataatta
     1141 agacaattac ttatacgaat ggcgacgata gtaccaccag ccgacattag caggtaatgc
     1201 aaatttagcc cgcgttatcg tttgctcatt ctgcagacaa catcaagcgc agtcgaccac
     1261 cggttcccca tatttggcat tgccaggcat cgcatcgctg gctcaattga ttcaaataag
     1321 cgttacctaa agtgcctaac ggaacaccat tactgatttg cacctggtgt tcgccggtat
     1381 ttaacgtcgc attcagaccc gcagaaacca gaatgagatt tttcagttcg cgatgataat
     1441 agccaactaa tagcggaaac tgccccggca gattggcctg acggagtaaa tggtttacct
     1501 gcttcaataa cgcgcccaac tctggcaacc gttgattttg gtgtgcaagc tgttcctgta
     1561 ataatccgtt aaacaatgcg cgtaataaca aggcagcaag tacgccatta tgtccagctc
     1621 gggtgacatc aaggcaataa aatgccagat cgttttccga aagtgcggca atatcaagag
     1681 ccaggccggg tttgtccgcg gcaaccaatt gacgataatt aacccggcaa tgggaaatca
     1741 cctgctgaac cggcggttgt agttcctgta ataatttcgc cgctgcggca gggttatcaa
     1801 ccattgcatc ccagtcgcga aaaagccttt cctcttcctc aacgcgcgaa ttaaacatgc
     1861 tgggatagag acaggcaaaa accatctcgc gcaagcgatt cagatcttta actggtttca
     1921 gcaaaacatc ttcaacgccc agacgtaacg ctttggcaat atctgccata ttttcagtgg
     1981 cagatatcac cagaactggg gtctggtcgc ctctgttacg tatatgctcc agcagtttaa
     2041 gcccgttcat tcgtggcatc gcgatatcac atatcatcag gtctggagtg aaacctccca
     2101 gcaactcaag ggcatccacc ccatcagccg ccagtaccgt tgtcgctccc aatgaggaaa
     2161 accatgaatc cagaagcgag cgaaatacct gctcatcttc aacaatgaga atctgttttc
     2221 cgaccaatgg ctgcgtcatg ttctctcccc tgactggctt tactcaatag tggcatgcta
     2281 ttgccacctg cgcctgtcag aattaactta agtgtagata aaaattctgg tcaaatgttg
     2341 gtgcgtacca acggcaaaag ttcgtccatt ttcctttcca ctgccagctg tccggccgca
     2401 atggcagcgt gcgcgcgatg gaaatcaagc gtagatattt gcgggcaaac aggttgaatc
     2461 agaatatcgg gcggatcacc tgccatgcgg ttccttttaa ggcggttctc cagcacctgg
     2521 atagaagtgg tcataatctc tgttgccgtt ggtgctgtca ccgcccgacg tgtcgttatg
     2581 ctgcctaacc tttctttcag acgcgcatgc cacggcagag aatcaccatt ctcgctattt
     2641 tcttcactga cattaaagga gagcaaatct tgttgcatca aatgagcatc gtgctgcagg
     2701 tcaaccgcta tcacaatatc agcccccaat gcacgcgtga gggaaatagg aattgggtta
     2761 acgactgctc catcaaccag ccagtagccg ttatgtgcaa cgggtgccat gagtcctgga
     2821 atactgcatg atgcgcgaat agcaagatgg agatcgcctt cagtaaacca taattcacgc
     2881 cccgtactta aattggtggc aacagccgca aagcgacggg aacaattttc gatctctgtt
     2941 tccggcatta tttcgcgata ttgattgaag acacgctcgc cgcgcagtaa cccaccgcgc
     3001 tgccaggaga ggtccatcag gcgtaaaacg tcccaataac tgaaagaggt tacccaatct
     3061 tccagcgcag ataatcgatc gcatgcatag gccgcgccca ccagcgaacc aattgaacat
     3121 cctgcaatga tatcaatttc aatacccact ttttttagcg cattaataac gccaatatgc
     3181 gaccaacctc tcgccgcgcc agatcccagc gccagcccta tttttatctt tctcattatt
     3241 cccgcaagat tcccctggaa tgcaatcgta gccacatcgc tactgctcgg ttaacatagt
     3301 gctactcagg cggttaatac gccgttattg tttccaggga gattatttgt gtctcagctt
     3361 tgtccctgtg gtagtgctgt cgagtatagc ctatgttgcc acccttatgt gtctggtgaa
     3421 aaggttgcac ctgatccaga acatctcatg cgttcgcgtt actgcacttt tgtgatgcaa
     3481 gacgcagatt atttaataaa gacctggcat ccatcttgtg gagcagcggc gttacgtgcc
     3541 gaattgattg ccggatttgc gcatactgaa tggttgggat tgaccgtatt tgaacattgt
     3601 tggcaggacg ccgacaatat cggatttgtc agttttgttg ctcgatttac cgaaggtggc
     3661 aaaacaggtg caataattga acgttcgcga tttttgaaag aaaacggtca atggtactat
     3721 attgacggta cacgtccgca gtttggccgc aacgatccct gcccttgtgg ttcaggtaaa
     3781 aaatttaaaa agtgctgcgg ccaataatgg ttgacggtac ggtttagcaa acactctcaa
     3841 caaggttttt ccagcaatgc attcactcca acgtaaagtt ctgcgtacta tttgtccgga
     3901 ccaaaaaggt ctgatcgcac gtattaccaa tatttgctac aagcacgagt taaatatcgt
     3961 acagaacaat gaatttgttg atcaccgtac cgggcgcttt tttatgcgca cggaactgga
     4021 agggattttt aatgattcca ccctgctggc ggatctcgat agcgcattgc cagaaggctc
     4081 cgtgcgtgag ctgaatcctg ccggtcgtcg ccggatagtg attctggtca ctaaagaagc
     4141 gcattgcctt ggcgatttgt tgatgaaagc caattacggc ggcctggatg tcgaaatcgc
     4201 ggcagttatt ggtaaccacg atactctacg ttctctggtt gagcgttttg atattccgtt
     4261 tgagctggta agccatgaag ggttaagccg caacgagcac gatcaaaaga tggcggatgc
     4321 cattgatgct tatcaacctg actacgtggt gctggcgaag tatatgcggg tattaacgcc
     4381 ggaatttgtg gcacgcttcc cgaataagat catcaatatt caccattcat tcctgccagc
     4441 gtttattggc gcacgtcctt atcaccaggc ctatgaacgt ggtgtgaaga ttattggcgc
     4501 aaccgctcac tatgtgaatg acaatctgga cgaaggccca atcatcatgc aggacgttat
     4561 tcatgtcgat catacctaca cagctgaaga tatgatgcgc gcaggtcgtg acgtcgagaa
     4621 aaacgtctta agtcgtgcac tatacaaagt actggcacag cgcgtctttg tttacggtaa
     4681 tcgaacgatt attctttaat cgccagcaaa aataactggt tacctttaat ccgttacgga
     4741 tgaaaattac gcaaccagtt catttttctc aacgtaacac tttacagcgg cgcgtcattt
     4801 gatatgatgc gccccgcttc ccgataaggg agcaggccag taaaaagcat taccccgtgg
     4861 tggggttccc gagcggccaa agggagcaga ctgtaaatct gccgtcatcg acttcgaagg
     4921 ttcgaatcct tcccccacca ccataattca ccacagcgat gtggtaactt cggtgagatt
     4981 gtgaaagttt gcactcatcg gggaagggtg agaaccttcg actaaggttc gattcgagcg
     5041 aaagcgagag aacgttgcca caggcaacgg cccgaagggt gaggagcgaa gcgacgaata
     5101 atccttcccc caccaccatc tcaagcatta cctcaaaatt tgaattaccc ctggtggggt
     5161 tcccgagcgg ccaaagggag cagactgtaa atctgccgtc atcgacttcg aaggttcgaa
     5221 tccttccccc accaccatca ctttcaaaag tccctgaact tcccaacgaa tccgcaatta
     5281 aatattctgc ccatgcgggg aaggatgaga agcttcgac
//
LOCUS       DAJOSY010000184         5231 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJOSY010000184 DAJOSY010000000
VERSION     DAJOSY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.184"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..814
                     /locus_tag="OHN27_004189"
                     /pseudo
     CDS             1..814
                     /locus_tag="OHN27_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            1177..2214
                     /gene="dusA"
                     /locus_tag="OHN27_004190"
     CDS             1177..2214
                     /gene="dusA"
                     /locus_tag="OHN27_004190"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCQ2288529.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDIDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2348..2590
                     /gene="pspG"
                     /locus_tag="OHN27_004191"
     CDS             2348..2590
                     /gene="pspG"
                     /locus_tag="OHN27_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCQ2288530.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2756..3739)
                     /locus_tag="OHN27_004192"
     CDS             complement(2756..3739)
                     /locus_tag="OHN27_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCQ2288531.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3822..>5231
                     /gene="dnaB"
                     /locus_tag="OHN27_004193"
     CDS             3822..>5231
                     /gene="dnaB"
                     /locus_tag="OHN27_004193"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCQ2288532.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDD"
BASE COUNT         1326 a         1205 c         1324 g         1376 t
ORIGIN      
        1 atataattat tgcaaaaata gatttgttta ttcaacgaat cacgcataat ttatggcatg
       61 cacgtaaaaa aatgtgtttt taattgagca aataaatgat ctcaaaatgt gggtcaataa
      121 gtatattgac gattgcactg atgaggattt aaacgatcgt gactttattg catcagttgt
      181 tgaccgggct atttttcatt tcgcgattaa tagtatatgt aatcttgggg ataataaaga
      241 tgcaatgccc attgaacaat gtacttttga tgtagaaact aagaatgacc ttccctccac
      301 ggttcagcta ttttatgagg aatctaagga taatgaacct ttagcgaata tacattttca
      361 agcaataggt tctggttttt taacgtttgt taatgcctgc caggaacatg atgacaacag
      421 cttaaaatta tttgcttcgc tgttaatttc actttcatat tctagtgcct acgcagattt
      481 atcagaaaca gtgtatatta atgaaaataa tgagagctac ctgaaagctc agtttgaaaa
      541 attatctcaa cgtgatatga agaagtacct gggagagatg aagcgtctgg ctgatggggg
      601 agaaatgaat tttgatggct atctggataa gatgtcacat ctggtgaatg aaggaacgct
      661 cgatcctgat attttaagca aaatgcgaga tgctgcacca caattaatta gcttcgcgaa
      721 gtcgtttgac ccaacctcaa aggaagagat taaaatactt acagacactt ctaaattaat
      781 ttatgatttg ttcggggtta aatcggagaa ataatatgtg aagttcttcg atagtatgga
      841 aggcattata taaaaggacc caatatttat tgggttcttt tttctctatc aatgctatta
      901 gcagggagat atatcaccag agtttaatgt gtgatttttt atttatcgtc gaacctggat
      961 tgtttatcat tggccttaac aaagttaacg gctaataaga ttatttccat cacttcgtga
     1021 gagcttcatg ccttgagagg atctcaattt tcttttgcaa tgagacaggc gcttcctgtt
     1081 gttatggtat agtaccccgc tattgagcct cctgaacagt gatgctgaat aacataaccc
     1141 catgatatat cgataaaata atctctacat ttgaaaatgc acggtaattc tgaaatgcaa
     1201 aaaatcaacc aaaccagcgc aatgcctgaa aaaactgacg ttcactggag tggtcggttt
     1261 agcgttgcac caatgctcga ctggacggac agacattgcc gctatttctt gcgtctgctt
     1321 tcccgcaata cgttgctgta tactgaaatg gtaactacag gggcgattat tcacggtaaa
     1381 ggtgattacc tggcgtatag tgaagaagaa catccggtag cgttgcaact cggcggtagc
     1441 gatccggcgg cgctggcgca gtgtgcgaag ctggcagaag cgcgtggata tgatgagatc
     1501 aacctgaatg tcggctgccc gtctgaccgg gtgcagaacg gcatgtttgg tgcgtgtctg
     1561 atgggtaatg cgcagctggt tgccgactgc gtgaaagcga tgcgcgatgt ggtgtcgatt
     1621 ccggtgacgg tgaaaacgcg tattggcatc gacgaccagg acagctatga atttctctgc
     1681 gatttcatca acaccgtttc cggcaaaggc gagtgtgaga tgttcatcat ccatgcacgt
     1741 aaagcctggc tttcggggtt aagtccgaaa gaaaaccgtg agatcccgcc gctcgattat
     1801 ccgcgtgtgt atcaactgaa gcgtgacttt ccgcatctga caatgtcgat taacggtggt
     1861 atcaagtcgc tggaagaggc caaagcacac ctgcaacata tggatggcgt gatggtcggg
     1921 cgcgaggcgt atcagaatcc gggtattctg gcggcggtag accgggagat ctttggttcc
     1981 tcggatatcg atgccgatcc ggtggcggta gtgcgcgcca tgtatccgta cattgagcgt
     2041 gaactcagcc aggggacgta tctcggtcat attacccggc atatgttggg cttgttccag
     2101 ggtattcctg gcgcgcggca gtggcggcgt tatttaagtg aaaatgccca taaagcgggt
     2161 gcagacatta atgtgctgga acacgcgctc aaactggtgg cggataagcg ttaacttttc
     2221 accaaaaaat agtcaaattc accacgccct gcgcaccgtc gcggggcgtt ttgctgttaa
     2281 atcaatagat tatttttggc atgattcttg taatgccagc aagagatttc atatttggga
     2341 gtgcatcatg ctggaactac tttttgtgat tggctttttt gtcatgctga tggtcaccgg
     2401 cgtttcgttg ctgggcatta tcgccgcgct ggttgtggcg acggccatta tgttcctcgg
     2461 cggtatgctg gcattgatga ttaagttgct gccgtggtta ctactggcga ttgcggtggt
     2521 gtgggttatt aaggcgatta aagcaccaaa agtgccgaaa tatcagcgtt atgaccgctg
     2581 gcgttactaa gggattgtgc ggatgatcac aacctaaggt tttatcctta gaacaaaata
     2641 ggaattgata atcaaatctg tcactattgc gcctctaaca gattcatcgt gctgtaccct
     2701 acatacagcc gaactataaa aagaaagggc ttcccaggtg gaagccctat ttcttttatg
     2761 gaatcaacag gctggaacct tgcgttgccc ggctttccag aatttcatgc gcacgctgcg
     2821 catccttcag cggatatttc tgctgctcgg cgacatcgac cttaatcaca ccgctggcaa
     2881 tcaaagagaa cagttcatta ctggcctcgg ttaattcctc ccgcgtggtg atatagcctt
     2941 gcagggaagg gcgtgtcaca tacaacgagc ctttctgatt gagaatgcct aagttcacac
     3001 cggtaaccgc acctgatgag ttgccaaaac tcaccattaa gccgcggcgt tgcaggcaat
     3061 ccagcgaccg ttcccaggtg tctctgccca cggaatcgta caccacgcgc actttcttac
     3121 cgccggtgat ctcttttaac cgctcgacca gattctcttc acgatagtta ataacctgcc
     3181 acgcgcccgc ttttagcgcg ctctgcgctt tttgcgcggt tcctacggtg ccgataagtt
     3241 tcgcgcccag ggcttttgcc cactggcagg caatcaagcc aacgccgcca gctgctgcgt
     3301 ggaacaggaa ctgctcatcg ggtttaattt cataggtttt gcgcagcaga taataaaccg
     3361 ttaagccttt caggaaggat gccgcagctt gctcaaaaga aattgccgca ggcagaatcg
     3421 ccgctttatc cgcaataatg ttatgcacag agctgtaagc gcctaacgcc gactgcgcat
     3481 agactacacg atcgcctgcc ttaatatgct ttacaccact gccgacttta ctcacgatgc
     3541 ctgctgcttc ggtgcctaat ccgctgggta gcgatggcgg cgggtaaagg ccgctgcgga
     3601 tatatgtgtc gataaaattg atgccgatgg ctttattttc gacctggatt tcattctccg
     3661 ccggatcggc aggagtgaac tctacggctt gaagtacttc cgggccaccg tgcttgtgaa
     3721 attcaattcg tgttgccatg tgtcctccag aacgtatcgt cagggtctgc ttcatatggt
     3781 aaagtttcga cccattcttt atctcggtaa ctccattcac tatggcagga aataaaccct
     3841 tcaacaaaca gcaggctgaa ccccgcgaac gcgatccaca agttgccggg ctgaaagtgc
     3901 ctccgcactc gatcgaagcg gagcagtcgg tgttgggcgg tttaatgcta gataacgaac
     3961 gctgggatga tgtagccgag cgtgtggtag cagacgattt ttacacccgc ccacaccgtc
     4021 atatctttac tgaaatggcg cgtttgcagg aaagcggtag ccctatcgat ctgattactc
     4081 ttgcggaatc gctggaacgc caggggcaac tcgatagcgt cggtggtttt gcttatctgg
     4141 cagagctgtc aaaaaatacg ccaagtgcgg ctaacatcag tgcctatgcg gacatcgtgc
     4201 gtgaacgtgc cgttgtccgt gagatgatct cggttgcgaa tgagattgcc gaagctggtt
     4261 ttgatccgca ggggcgtacc agcgaagatc tgctggatct ggctgaatcc cgcgtcttta
     4321 aaattgccga aagtcgtgcg aacaaagacg aagggccgaa gaacatcgcc gatgtgctcg
     4381 acgcaaccgt ggcgcgtatt gagcagttgt ttcagcagcc acacgatggc gttaccgggg
     4441 taaacaccgg ttatgacgat ctcaacaaaa aaaccgctgg cttgcagccg tcggatttga
     4501 tcatcgtcgc cgcgcgtccg tcgatgggta aaacaacatt tgcgatgaac ctcgtcgaaa
     4561 acgcggcgat gttgcaggat aaaccggtac ttatcttctc gctggagatg ccatcagaac
     4621 agatcatgat gcgttctctg gcgtcgctgt cgcgcgttga ccagactaaa atccgtaccg
     4681 ggcagctcga tgacgaagac tgggcgcgca tttccggcac catgggtatt ttgctcgaaa
     4741 aacgcaatat ctatatcgat gactcctccg gcctgacgcc aacggaagtg cgttcccgcg
     4801 cacgccgtat tgcccgtgaa cacggcggca tcgggcttat catgatcgac tacctgcaac
     4861 tgatgcgcgt accggcgctt tccgataacc gtacgctgga aattgcagaa atctctcgct
     4921 cgctgaaagc actggcgaaa gaactgaacg tgccggtggt ggcgctgtcc cagttgaacc
     4981 gttctctgga acaacgtgcc gacaaacgcc cggtcaactc cgacctgcgt gaatctggct
     5041 ctatcgagca ggatgcggac ttgatcatgt ttatctatcg tgatgaggtg tatcacgaaa
     5101 acagtgattt aaaaggcatc gcggaaatta ttatcggtaa acaacgtaac ggcccaatcg
     5161 ggacggtacg cctgaccttt aacggtcaat ggtcgcgctt cgacaactat gcggggccgc
     5221 agtacgacga c
//
LOCUS       DAJOSY010000185         5176 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJOSY010000185 DAJOSY010000000
VERSION     DAJOSY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.185"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(1..2683)
                     /gene="traG"
                     /locus_tag="OHN27_004194"
                     /pseudo
     CDS             complement(1..2683)
                     /gene="traG"
                     /locus_tag="OHN27_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
     gene            complement(2680..4053)
                     /locus_tag="OHN27_004195"
     CDS             complement(2680..4053)
                     /locus_tag="OHN27_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HCQ2288533.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINSEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(4053..4415)
                     /gene="trbJ"
                     /locus_tag="OHN27_004196"
     CDS             complement(4053..4415)
                     /gene="trbJ"
                     /locus_tag="OHN27_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HCQ2288534.1"
                     /translation="MLPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGRKYRD
                     IPAEMSYAPGEQIKGGQE"
     gene            complement(4345..4890)
                     /gene="trbB"
                     /locus_tag="OHN27_004197"
     CDS             complement(4345..4890)
                     /gene="trbB"
                     /locus_tag="OHN27_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HCQ2288535.1"
                     /translation="MSLIKSLLFTLLLAAAAVQASTRDEIERLWNPQGMAAQPAQPAA
                     GTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(4877..5161)
                     /gene="traQ"
                     /locus_tag="OHN27_004198"
     CDS             complement(4877..5161)
                     /gene="traQ"
                     /locus_tag="OHN27_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HCQ2288536.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMAEQQEGGHVSH"
BASE COUNT         1208 a         1450 c         1261 g         1257 t
ORIGIN      
        1 ttttgtagtt cagaatactg tcttcctaca atattttctt cgccacgtat gctgctctga
       61 gtatctccga tattgcctcg gtattctgtg accatattat caacctgatg ttttacatca
      121 ttacggatat tactgtcctg cgttctttgc tcaataatgg cctgatgccc ctgatgatca
      181 gcaataatat cctggctgcc accacccgaa ggtacgctct ccattccttt accgatgtcc
      241 ccacgggatt cacgccaggc attatcaaca ccaggctgca cctgttcctg aacaaaagac
      301 cacgccatag cccgacggcg ttctgcaatc tccggtgaac tggtgtttgt cagaatagct
      361 tcggcatcct gcggcgcgtg tttcatcaca tactgtgcaa actgttgcga caggtcttca
      421 ctcatctgcc cactcatgct ttcagtacgg gatgccattt cagcatattc atggctgcgg
      481 gtcatattcg tcgtgtactg gtcgtaactc tgttttgctg agttcagggc tgcagacagc
      541 tggtccacac gggaatcggc attattgtcc gtatggctgc cggattcact gaccttgcga
      601 ctggtaaagt aatcgctggc ctctttaaag tcttgtgtag ccctggcatc gatatcatgg
      661 cgagcatcat ggctggcccg tgaaccactg ctggcctgat gcgcatcctc atcactccag
      721 tcaatacttg cccgaccacc acctctaaca cctacgccac caccaacacc aagaatttta
      781 ggtttaaccc cccattcagc atgagcatcg ccatacatcc ccgcagaacc tctcgtactt
      841 cttgatgcca gttctcttgt cgcctgctca ttactgatat tatgcgcctt cgcatagctt
      901 tccactgcgc tgcgcatacg gctggccatc atggagtcct gtgcgctcat cgtgctgtca
      961 gcaccaccgg tgacagaatc actgctcccc cggttagaac caaactgact gagcgagttc
     1021 caggcactgg caatactgct gctgaaccca tgcagggcac tttctgctct gttcgatgct
     1081 tcccgggcca tttcctgttg tgccgccgca atctgacgcg ttgcattgat gccgaccggt
     1141 aaccgggaca tcgctccgct tgcatccatc accatattac cgtcacgggt ctgtgtagcg
     1201 gttgcgccac tgccggtctg gtacatcatc tgaccaaacg acgtggtgct gttggtgctc
     1261 cagctgaatc cgttcacgtt ttccgtctgc atgttgccgt aggagtaatt accgtcaacc
     1321 acactacctg ccgcactggc agtcgggctg atagcagagg aagcgaaatg gctgtacaca
     1381 ctggaaaatc cggccccgag tcccctgacc atcatccagg ataatggcgg gatcatcatg
     1441 gaaagatacc cggcagtgga agccagatcg gagtatttca gctgtatctg tgaaatttcc
     1501 gagagcacca ccggtgcacc attctgcttc gcataaaatg tcatggcgct gttcagaatg
     1561 gcgtacaaca ttggccatga ctgcaaccac atcagggcaa acacatagcc tttcaggacg
     1621 gacagcgtca gtttgttgaa gacagccgcc agtaccagaa gcgggaaaat accaatggcg
     1681 atccccgtca ggatggtctg cgtcatgggc agtgtccgca tcgccacatg accgatggag
     1741 acatgtgcca gacgttgctt ctccatcgat gacgtggtgg ccagattcac caggctggct
     1801 gagtcaccat tacgtgctgc ataactggtg atcccctcct tcagcgcatt aatggtgaca
     1861 ttctggcgga tgatctggct ggctgattta cttgagtcat agaagtaacg gtaactgtcg
     1921 ccaatcagcg tactgaacaa caggttcgga tcagggcgac caccaaataa ctgctggcat
     1981 aataatgcca ggtttttccg ccactctgtg tgtccagatt cagcttgtct ttcaacgaaa
     2041 ccgacgcatc cttacaggtt acgaaattat tattgctgtc ataaacgccc cgcagcggac
     2101 tgggacggga aaaaatcagc gtgtagggat tagaggaggc catcagctcc tccagcgtgt
     2161 atttgtggtt caggtaaata tcccccatca cgcagttctg cacataatcc tggaaaagat
     2221 tggcgatttc cggattacga gacaaaaagt cagtgctttt tgataccagt tctgcaccaa
     2281 acagcatccc cgttttgctg taggtgaaac tgtccggttg cgtgtagatc atctcgtaac
     2341 tggccaccat tgcatgcccg atacgggtcg tcagtgaaag tggcatcgcc agtccgaccg
     2401 gcacattatc cgcccggtga actttgacca ggtcactgtt atcaataatc tgcaccgatg
     2461 tgcggatatt aaccagcagg ctgataagta caaacaccgc cgcccagccc agcaaatcca
     2521 tcacgttatg gcgctgtacc cacataaagg ccaccgcaac aacagacagg gtgagagcaa
     2581 ttttctcaat ggaatcccac gtccgggttc ccataaaggc ggcaatggca ttcaggttat
     2641 tccgcaacca ctcaccaccg gcaatcacat aaacttcatt cacagcgtgc tccctccgaa
     2701 gtgatagttg ttctggtaac gactgagcat gcgggcggaa agctgctgac gcatgtagct
     2761 catctgacga tcgacaacca gcagcgcatc ctgctgtacc tgcacctgtg actgaaacgc
     2821 cgcaatctgc cgggtggcat cattcatatt gtcgttaata tgttcgataa ctgcttcgtc
     2881 ataatttccc gtggccacca tcgcccgggc ctgctgtatc agctcctgaa tgtactgcag
     2941 caggatgtcg taaccgatat agtccgtcag ctggtaaatc atgctgttgg aaacaccgag
     3001 catctgcggg tcaaccaggt atttgaagac ggggatggtg gtactggaaa taaagccttt
     3061 ttccttgtca tccagaggcg tgtcactgac cgccttgttc tgaatgctgg ccagaagttt
     3121 agtaatctga gacttcagtg cattatcccg gcttatagtc accggtgtgt ctgccaccac
     3181 tttcaggcat ttatcagaat cgttgcagtg ataaattttt gctgtgccgc cttccatcat
     3241 ggcccggata attgagcggt cagtggttct ggccgacagc ggtgtgattt cgccgttggg
     3301 accaaacacc agtgagccgg tcagcgtcat cacaaactct ttcagctctt tgttgccgtc
     3361 aaacattctg tttttggaaa gcgcattcca catgatgtta atgtttttgg tcacccgctc
     3421 cttatccttg tcgctggctt tatccctgac gctgtcagat ttcccgccaa cggtacatcc
     3481 ctgccgggaa gcagcccagt cagcaaaaat attgctctca ccggcaatgt cctgacagac
     3541 tttttgctgg gacacctgcg tccgggggaa aagcccgcca ataatcccct gcgccgcctg
     3601 gcaggaactg aggttcatac tgttaatgtc gcttgccatt ttctgcagga agtcttttgc
     3661 ggttttgatt tccggcaccg ttgtctgcag ggcaaggtca aagaagtaac cggcagcatt
     3721 gctcataatc tgcttaacaa aacgctgcaa ctgctcgcta ttaataaagc tgaacgagcc
     3781 gagataggcg tcgatgccgc cacatccggc attgatatcc ggcagcgtca tggaaatcag
     3841 ctgaacattt ttgacctgag tacgggcata cagggagcca ccgtaagcat aaccggcggc
     3901 ctgcccctgc cagacgcccg gctgtgtggt attggaggcg aagcccagct tgttaaagaa
     3961 ctgattcatg tcgctgttca catccgctga cgctgcgggt gtgaccgtca gtaaagcagc
     4021 acaaagaacc agaagaggtt taatgcgtgg catcattctt gccctccctt aatttgttct
     4081 cccggcgcat acgacatttc agcaggtata tcccgatatt tccgtccagt cctgcggctg
     4141 taatatattt cagacaccga aatccatccc acaattgccc ccacaaaaaa cacaacagaa
     4201 acagaaaagg taaggcacag cgaaacgaca atttctacag ggctggcggg ataaaaaagc
     4261 ccctgatgga taaaggggat attcaggctg acgattattc ccgtcgcgat gccggatatt
     4321 ccggcaatga gtaacaccac ctgcttattt cgcacctttt ttccctccgt acatctgcag
     4381 aacggtatcc atccgcgcca taaaaccggc agcatccgtt gccccctgta aaagcggtaa
     4441 tgcctcaagc gtgttgacgt tgaccaggaa ggtggtcggt gtggccaccg ggatattcgg
     4501 gaagaaggtc tgcatcacgt ccggtggcac cggcaatgcc tccggaaagg cggtatcccc
     4561 ctgaccatcc agggtgtagg gaaaaacgga aaagccgtac tgctgtgcca gctgtttcag
     4621 taccgggtca aactggtgac agtaagggca atgcccctgc ataaacagca ccaccttcca
     4681 gtcggccagg ttaacctgcc tgccgttgct gaggcggaac cagcgggggg ctggcttttc
     4741 tgtcctggct gacgtgcctg ctgccggttg tgcaggctgc gcggccatgc cctgcggatt
     4801 ccagagtcgt tcaatttcat cccgggtgga ggcctgcacg gcagcggctg ccagcaacag
     4861 ggtgaacaac agtgatttaa tgagagacat gtccgccctc ctgctgctct gccatcatgg
     4921 cctgttgcct ggcttccagt gcctcacgct cttcccggct gacacgggca atccatgcat
     4981 ccagcacctg gtatgcgccg aacagcacaa ggatggccgc aatcaactcc gccagaaaaa
     5041 aagccatcca gggtttgctg taaacaagac gggcgacgat atgaaaccag acacccaggg
     5101 aaaataccca cattcccgta atatcaagcc ggggtaaaga gaatctgcgt ttacttatca
     5161 tgttaaactc cggtta
//
LOCUS       DAJOSY010000186         4980 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJOSY010000186 DAJOSY010000000
VERSION     DAJOSY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.186"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(64..846)
                     /locus_tag="OHN27_004199"
     CDS             complement(64..846)
                     /locus_tag="OHN27_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HCQ2288537.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDTWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFLMPESDAVTMLKNRQA"
     gene            complement(848..1261)
                     /locus_tag="OHN27_004200"
     CDS             complement(848..1261)
                     /locus_tag="OHN27_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288538.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGALHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWGEH"
     gene            complement(1635..1928)
                     /locus_tag="OHN27_004201"
                     /pseudo
     CDS             complement(1635..1928)
                     /locus_tag="OHN27_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2545..3522
                     /locus_tag="OHN27_004202"
     CDS             2545..3522
                     /locus_tag="OHN27_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCQ2288539.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3807..4547)
                     /locus_tag="OHN27_004203"
     CDS             complement(3807..4547)
                     /locus_tag="OHN27_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCQ2288540.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4668..4856)
                     /locus_tag="OHN27_004204"
     CDS             complement(4668..4856)
                     /locus_tag="OHN27_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HCQ2288541.1"
                     /translation="MESEIFSCLLRASAGFFLRFCLSWHYPNSPKNGKPVLSPHHDWL
                     KTGSLRQNAPLCIWFYPQ"
BASE COUNT         1188 a         1339 c         1266 g         1187 t
ORIGIN      
        1 cacatattgc agtggtctct ttttttacag aataaatcac aattcataat tatgacttat
       61 gatttatgcc tgcctgtttt tcagcattgt gacagcatcg gactcaggca tcaaaaactg
      121 cacccggtgc cgtgcagcca catccagtgc aaacaccttc gtgtagacct ccgttgagct
      181 gatggactta tgccccatca ggctctgcag gaccttcagc ggtataccgg catacagcat
      241 gtgcatcgca taggaatggc ggaacgtatg tggcgtgacc gggacagaga acgtcacacc
      301 atcagcggca gcggcggcaa ccgcctcccc aatccaggtc ctgaccgttc tgtccgtcac
      361 ttcccagatc cgcgctttct ctgtcctgcc tgttcgttta ttacgccgtt ccatggggat
      421 tttcagtgtg gccaccatcg tctgcagctg gctgacgtac caggtgtcgg aaagcggaac
      481 cagccggtga gtctgctgac cggcgggcat tcttcctgcc gtcctggcgg ctttttctgt
      541 ccgctgcttc agagtggcca gctgcacaaa cggatacgga ggcgtgagcg aaaaatcccc
      601 ccgcgtcagc gccagtgctt cattaatgcg cgcaccggtg ttccacagtg tggccagcag
      661 catcttgcgg cgcagatccg ggacgtaatg gagcagggcg ctcacttcag gggccagcag
      721 gtattttggc agttcatcat gaaccatcga catctggcgg agtgccagcg ctgccggata
      781 atcgatggca acaggaagct gagcggcatc ccgggcatgg tggatcggtg ccggcaggtg
      841 ctgcatatca gtgttcaccc cacggggaaa cgtcgggcag gttttccaca acacggtact
      901 ttcccggata acggagctgg ttaccgccac gcgtccagac ggtgacaaca aaaccccctc
      961 cctggttgtt ttcggggagc aggcgatcgc actgctcact cagcatgtgg atcacaaacg
     1021 gcgcatcgct gccgtcttca aataccagtt ccagcgcatc gcggccatgc agtgctccct
     1081 tactgatgat gacgtactca gccccccgca tctccctgag taaatgtttt gccgcatccg
     1141 ggacaagcag cctggctgcg ccggcattcc agctgaggta gacatatccg gcctgcgcct
     1201 gtacagactg ccagtaatcc gttctggcga cagcctgacc ttcattctca ataaaaaaca
     1261 tgggtgactc cttttttctt ttccgggcag ggacggtcat gcgataacgt catccatcat
     1321 cactgccctt accgtcacgc cgtctgataa catcatgccg ttatcatcat caccatgata
     1381 acaagcatcc atataaccac gccgttaaat tactggagag ccgtttccgt cagatccgac
     1441 tttcctgaat atcacaactt tccggaagtg tatcacgctg aggaaatcat catatcgctg
     1501 ttttccgccc ctgcgcggcg catcacaggt cagggagatg acacaacata tagtgtcatc
     1561 agcagctgaa tccggcacca catgttgtgc ttacagggtg tggcgctgtg cggaggggga
     1621 ggaagatccg taaccgcaaa ataagcggaa gcctggcgaa ggatatcgtt actgcggcgc
     1681 agttcacgat tttcgcgttc cagctctttc agacgctgac gttcagcggt ggtgagccca
     1741 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc agacacgcag ggtctctggc
     1801 gtacagccaa tctttggagc aatggaacaa attgccgccc cttgtgagtc atattcgccc
     1861 tggctttcca gaaccatacg aactgcccgt tgacggactt cgggggaaaa acgagtattt
     1921 ttagtcatcc tgtttacatc tttctcagga agtttagtct ccaggatttc cggggcggtt
     1981 cagtactgca gattccgtca aaaattacct gtaaagacag acatcagaaa aacagtgctc
     2041 gccgcagacc ggtgaccggg cgaagcccgc gaaccggcga ggaagcgcaa tgtatctgta
     2101 gttacagtgc gcaggtacgc cctgaagccc ttataagagg ttgtgtatgc tgtttcccct
     2161 gggatgattt atccacatat ccagaggttc gatccagtaa acagatccat gaatgatcaa
     2221 caaaggatcc attaaagatc cccatatctc tgcaaacctt gtcactcatg ggccgggacc
     2281 acgatcacat aagctgtggc atgttactga taaactgtaa catgctaatg ataagctgta
     2341 ttcagtaatc catatactga agtaagttaa tgacataaac tatggtcagt atgccagact
     2401 cagttgttaa atacaggctg caggtttttc ttcagtcagt tagcggggct ctgacacacg
     2461 atttgctgtt tattctttta ctgtccacag gcaggaggct ttctggaaaa cgaaaattca
     2521 gacatcaaaa aactgttcgg cgaggtggat aagtcgtccg gtgaactggt gacactgaca
     2581 ccaaacaata acaacaccgt acaacctgtg gcgctgatgc gtctgggcgt ctttgtaccg
     2641 acccttaagt cactgaagaa cagtaaaaaa aatacactgt cacgcaccga tgccacggaa
     2701 gaactgacac gtctttccct ggcccgtgct gagggattcg ataaggttga gatcaccggc
     2761 ccccggctgg atatggataa cgatttcaag acctgggtgg ggatcattca ttcctttgcc
     2821 cgccataacg tgattggtga caaagttgaa ctgccttttg ttgagtttgc aaaactgtgt
     2881 ggtatacctt caagccagtc atcccgcagg ctgcgtgagc gcatcagccc ttccctgaag
     2941 cgcattgccg gtaccgtgat ctcgttttcc cgtaccgatg agaagcacac ccgggaatac
     3001 atcacccatc tggtacagtc agcttactac gatactgagc gggatattgt gcagttacag
     3061 gccgatcccc gcctgtttga actgtaccag tttgacagaa aagtccttct ccagcttaag
     3121 gcgattaatg ccctgaagcg acgggagtcc gcccaggcac tctacacctt tatagagagc
     3181 ctgccccggg atccggcacc gatatcgctg gcgcggctgc gtgcacgcct caatctgaag
     3241 tctcctgtat tttcccagaa ccagacggtc agacgggcaa tggagcagtt gcgcgagatt
     3301 ggatatcttg attacacgga gatccagcgg gggcggacaa aattcttctg tattcactac
     3361 cggcgtcccc ggttaaaagc gccgaatgac gagagtaagg aaaatccgtt gccaccttca
     3421 cctgcggaaa aagtcagtcc ggagatggcg gagaagcttg ccctgcttga gaaactgggc
     3481 atcacgctgg atgacctgga aaaactcttc aaatcccgct gaacataaac tgtagtcagt
     3541 gaagagcgct cctttactga ctacagctta tattaaccgg tgcagtgagt ggtctgctca
     3601 ctgcagttta tattctgttt cctgcagtgc tgtctgtagc tgagctgcca tctgcctgtc
     3661 ccttacgtaa gtgatcccgt aacctgatgc tgaggcattg ctcccttcat aaaacatgac
     3721 ttactcacta cagcttatat acatgctcca gcttatgtta tgcctgttct gctgaccaca
     3781 gcttatataa ggaagcgcgt atgccgtcac ctcaggggag gcagtgtacg caggatctcc
     3841 gcagcatccc gcccgtcacc tgtgaaaggc actgccagcg tggcagccat atccagtgca
     3901 aacactctgg tataaacctc catcgaacgt ggatccctgt gaccagccag tgcctggatg
     3961 actttccggg gctggcggtg atagagcatg tgcatgatat agctgtgccg gaaggtgtgt
     4021 ggtgtgaccg gaatcgaaaa gtgtactccg tcagcttcgg cccgtctgac agcctgcttc
     4081 agccagttgc gcatggtttc gtcggtcacg gcccataatg gttcacgacg acggggccgg
     4141 gtggtgatca tccagctttc catctgcctg acatagctta tatctgtcag cggaaccagg
     4201 cgcacttcat cttttggcgg gcgtccgcgt cgcgcacgca ctttttcgga caggatccgc
     4261 acaaacggtc ttactccatt caggtcaaat gattccggcg tcagcatccg ggcttcgcca
     4321 atacgcattc cggtattcca gagggtggcg aacagcatat ggtgacgctg atccggcata
     4381 tagaagagaa gggcactcac ttccggggcc agcaggtagg ccggtgtggc ccccgtggaa
     4441 gtggccattc ttctcaggga gagcgctgtt gcaaaatcca cccccggcgc aatgggtaac
     4501 agggagacgc gttcaggtga attctgcagg ggaatgacat tgttcatgag ttatttttca
     4561 cgtagcacac gcactgttta tggatacagg tattctgacc tgaacgcccg gatccttcca
     4621 cgacaataaa tcgcagcgcg cggatccttt tgtttgctta tcacgcatta ttgcgggtaa
     4681 aaccaaatac ataaaggcgc gttttgccgc agggatcctg ttttaagcca gtcatggtgc
     4741 gggctgagga ctggtttacc gtttttggga gaattggggt aatgccagga caggcagaaa
     4801 cggagaaaaa atcctgcaga cgcccttaaa agacaactga aaatttctga ctccatattc
     4861 ctctggtttc agactgctta atcacatcat caacctgata cataataaac agattctgtt
     4921 cacagaatga acaattaagt aaatggttag attatgcgaa aaagtacaga tatttctcac
//
LOCUS       DAJOSY010000187         4960 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJOSY010000187 DAJOSY010000000
VERSION     DAJOSY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.187"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            4..939
                     /locus_tag="OHN27_004205"
     CDS             4..939
                     /locus_tag="OHN27_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCQ2288542.1"
                     /translation="MEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAA
                     KVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALD
                     IEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTD
                     LIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNF
                     SGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPF
                     PSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1148..2113
                     /gene="lipA"
                     /locus_tag="OHN27_004206"
     CDS             1148..2113
                     /gene="lipA"
                     /locus_tag="OHN27_004206"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCQ2288543.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2214..2417)
                     /gene="tatE"
                     /locus_tag="OHN27_004207"
     CDS             complement(2214..2417)
                     /gene="tatE"
                     /locus_tag="OHN27_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCQ2288544.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2546..3334)
                     /locus_tag="OHN27_004208"
     CDS             complement(2546..3334)
                     /locus_tag="OHN27_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCQ2288545.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3427..3810
                     /gene="crcB"
                     /locus_tag="OHN27_004209"
     CDS             3427..3810
                     /gene="crcB"
                     /locus_tag="OHN27_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCQ2288546.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3864..4073)
                     /gene="cspE"
                     /locus_tag="OHN27_004210"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3864..4073)
                     /gene="cspE"
                     /locus_tag="OHN27_004210"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCQ2288547.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4248..4808)
                     /gene="pagP"
                     /locus_tag="OHN27_004211"
     CDS             complement(4248..4808)
                     /gene="pagP"
                     /locus_tag="OHN27_004211"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCQ2288548.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1331 a         1251 c         1098 g         1280 t
ORIGIN      
        1 caaattgaac cctgtttaag cagaaagtcg tcagaaggta aaccacaaat atttacaacc
       61 ttacgaaata ttgatcttaa ccttctgact atttttgaag ctgtatatgt acataaaggg
      121 atcgttaatg cagcgaaagt gcttaatctg accccctcgg caatcagtca gtctattcag
      181 aaactgcgcg ttatattccc tgacccattg tttattcgca aaggccaggg tgtcactcct
      241 accgcatttg cgatgcatct acatgagtat atcagtcagg gccttgagtc cattcttggc
      301 gcgctggata tcgaaggaag ctatgataag caacgaacga taactattgc gaccactccc
      361 tcggtcggag cactggtcct tcctgtcatc tatcgggcga ttaaaactca ctatccgcag
      421 cttttactgc gcaacccgcc catcagcgac gcggaaaacc aactcagtca gtttcaaacc
      481 gatctcatca tcgataacat gttttgcacc aatcgtacgg tgcaacatca tgttctgttc
      541 accgacaata tggtgttaat ttgccgcgag ggaaatccac tactctcttt agaagatgac
      601 agagagacta tcgacaacgc tgcgcatgta cttctgttac cggaagagca aaatttcagc
      661 ggtctgcggc agagagttca agaaatgttt ccggaccggc aaatcaattt caccagctac
      721 aacattttga caatcgctgc actggttgcc aacagtgaca tgttagcgat tattcccagc
      781 cgtttttata acctgtttag ccgctgctgg ccgctggaaa aactgccttt tccgtcctta
      841 aatgaggagc aaatagattt ctctatccac tacaacaaat tcagcctgcg tgatcccatc
      901 ctgcacggag taatagatgt tatccgtaat gcattttgaa aaagtattgt tacagccaga
      961 tcacagcgca gaacgataaa atggcacaat ccaacaacaa ttttacattt tcgcgaccgc
     1021 tttggctgct ttcaggtccg tttcaatgat atactgccag tcgttaattc aaaaatagtt
     1081 gataattaca acaatctatt gaattgaaac gctttccttc gtaattcgca actggaacac
     1141 gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg atgccgataa
     1201 gatggccctt atcccggtta aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc
     1261 ggaatggatg aaaatcaagc ttccagcgga ctctacacgt atccagggca tcaaagccgc
     1321 aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc tcctgcccta acctggcgga
     1381 atgcttcaac cacggcacag caacgtttat gatcctcggc gctatttgta cccgccgttg
     1441 tccgttctgt gatgttgccc acggtcgccc ggtagctcct gatgccaatg aaccagtgaa
     1501 actggcgcag accattgccg atatggcgct gcgttatgtg gttatcacct ccgttgaccg
     1561 tgatgacctg cgcgatggcg gtgcccagca ctttgcggat tgcattactg ccattcggga
     1621 aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat ttccgtggtc gtatggatcg
     1681 tgctctggat attctgactg caacgccacc agatgtgttc aaccataacc tggaaaacgt
     1741 accgcgtatt taccgtcagg tacggcctgg tgcagattac aactggtcgc tgaagctgct
     1801 ggaacgcttt aaagaagcgc atccggaaat cccgaccaag tctggtctga tggtgggact
     1861 gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac
     1921 gatgttaacg ctggggcaat atttgcagcc aagccgccat cacctgccgg ttcaacgtta
     1981 cgttagcccg gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg gctttaccca
     2041 tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc gatttgcagg cgaaagggat
     2101 ggaagttaag taatcctgta ataattactt acatctgtct gataaaaaac ccgctttttg
     2161 aagcgggttt tttgtatcga acaagatatt gagggagcgt cctgctcgcc acgtcactct
     2221 ttatgagaga gcttttcagc ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg
     2281 tcatcattca tcgccttctt gaacccttta atggccgctc caaggtctcc gcccagcgta
     2341 cgtaacttct tagtcccaaa cagcagaacg accagcgccg caactaccag cagtttggta
     2401 atactaatct cacccataga taccttcttg acatataacc ggcaacagaa cgcgccttaa
     2461 aaaccagaga atattaacgg tcattcgacg cgcgcacaca atagcaatct gtaacaaggt
     2521 gaatcaagcg ccgagtaaaa aaacatcata ataattgcgg cggcgcaaag cgacggttgt
     2581 ttaagacggg cagttgcgcg cgcacctgac gcacacgttc gggcgtcact tccgccataa
     2641 tgagtgcagg catttctgac gctgccgcaa tggtgacgcc aaagggatct ataatccggc
     2701 tttgaccgat atttttgttc ccgcactccc ccgccgccac catataacag gtggtatcca
     2761 gcgcacgagc ggcaagcaac gttgaccagt gatgctcttt gagcggcccg cgaacccagg
     2821 cggcaggaag taccaggatt tcagctccct gtaatgcctg tgccagcgcc agctctggaa
     2881 agcgtaagtc ataacaggtc atcagaccga ccttcatccc ttccacctcc agtaacggag
     2941 cgatttcatt accagcatca acacggcgtg attcctgaat ggcaaatgca tcatagagat
     3001 gcagtttggc ataacgggcg acgatgtttc ctgcctgaag tgccaccagc atattccatg
     3061 cgcgccccgg cgttgaagga acatgaatcg tcagaattgt cgtcatcatg ttacgtttac
     3121 tttctcgccg taaacgtccg aggaattcgc cttccagcag ctgtgctgat ttaaccgata
     3181 gatctgcatc atgatcatcg cgcgccagca atgcttccgg caggacaaac agcgatacgt
     3241 cgttttccgc cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt tcccacacag
     3301 atgtaacagc aaactgtcct gcggcaacca gcattgccac ctcctgtcag agtcgtaaaa
     3361 ttcgccaacg acgattattc ggcgttacac ttatcactca tacaaatcaa atagcaggat
     3421 tttgcagtgt tacaacttct tttagcagtt tttattggcg gtggtacggg aagcgtggcg
     3481 agatggctgt taagtatgcg atttaaccca ctgcatcagg cgattccgtt ggggacgctg
     3541 acagcaaacc tgattggggc attcatcata ggaatgggat tcgcatggtt cagcaggatg
     3601 acgaacattg atccagtgtg gaaagtatta atcaccaccg gattttgtgg cggtctaaca
     3661 accttctcaa cattttcggc agaagtggtg tttttgttac aagagggccg ctttggctgg
     3721 gcattactga acgttttcgt caaccttctg gggtcttttg ccatgaccgc actggcattc
     3781 tggctgtttt cggcctcaac cgcacactaa agaccaaaaa aaacccgctg attaagcggg
     3841 ttttgaattc ttgctgacgt atcttacaga gcgattacgt ttgcagcaga agggcctttg
     3901 gcaccgttag tgatttcgaa ctctacgcgc tgaccttcag caagagtttt aaaaccatta
     3961 gtctggattg cagagaagtg tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa
     4021 ccgaatcctt tggactcatt aaaccactta acgttacctt taatcttaga catcaaaatt
     4081 acctttacat gaaaaataga cacaaatgct gtgtcggtta ccagtacacc aattgtggta
     4141 cgctttgtcc agtgtaactt tactaaaaag tgataaaagt cgcgttattt ttgatgttac
     4201 ttattttagc tattgatttt aaagaagtta ctaaaacttc atttgtctca aaactgaaag
     4261 cgcatccagg caaagtacac attgccattg ttgtaggtac ccggaatgta ggtcatctga
     4321 aaagtcactg ggccataacc cacggaggcc aatggcagta gaaccgggag agggatgtaa
     4381 ttccagttat cgcgtgccgt tacgccagcg gtgaatccca gacctaaatg aaaattttca
     4441 tccgccagcg gtcgccaggt actttcccat ccgtatccgg caatcggttc ccatttgttc
     4501 cacgagtcct taaatgccat ggcatacagg ccatgccagt ttcctttttc atcccaacgc
     4561 gacaggccaa aaccgccacc ccacggtcgc tcgttatagc gatcggtttt ttctttgtcg
     4621 taagcgaaac gtgcatgcca ggtgatggca ggaatatata aatcataatg ttcaggctgt
     4681 tgccaggttt gtgcaatatt ttctctaaac gttgtcatcc actcatctgc gttagcaaaa
     4741 actttaccaa cgctgattaa ctgaataaaa acaaaggaaa agatagcgac atatttactc
     4801 acgttcattt gtgaccataa aacatttatc aaaaatctac tactagcata gcaaagctac
     4861 aattaacata accttaatag acccaacata aagaataatc tgaatagctg ctctatttat
     4921 ttccgcttgt tcaagttaaa tatttccgcg catcgattaa
//
LOCUS       DAJOSY010000188         4936 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJOSY010000188 DAJOSY010000000
VERSION     DAJOSY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.188"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            86..2044
                     /locus_tag="OHN27_004212"
     CDS             86..2044
                     /locus_tag="OHN27_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCQ2288549.1"
                     /translation="MSVTESKVKTERKSSRKPAKTQETVLSALLAETAEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTERQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWGWCAGRMEAVGECREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQMQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSSPARIYLECGHYSLTSAAPSGKEGTAFMALMAEHFRL
                     AALLPDEWERDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDTTERTDNLADAA"
     gene            2099..2533
                     /gene="psiB"
                     /locus_tag="OHN27_004213"
     CDS             2099..2533
                     /gene="psiB"
                     /locus_tag="OHN27_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCQ2288550.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNVGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVSDIIHILMAEGGQA"
     gene            2530..3249
                     /locus_tag="OHN27_004214"
     CDS             2530..3249
                     /locus_tag="OHN27_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCQ2288551.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(3261..3449)
                     /locus_tag="OHN27_004215"
     CDS             complement(3261..3449)
                     /locus_tag="OHN27_004215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HCQ2288552.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            3529..3687
                     /locus_tag="OHN27_004216"
     CDS             3529..3687
                     /locus_tag="OHN27_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCQ2288553.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            4378..4584
                     /locus_tag="OHN27_004217"
     CDS             4378..4584
                     /locus_tag="OHN27_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCQ2288554.1"
                     /translation="MHIPQNFLPRSRQKLLRSAPGQSFPAVAVMPLAAKVLKQVMVNT
                     GGNKEQVPAKRQNRGSRNSTDDGG"
     gene            4609..4896
                     /locus_tag="OHN27_004218"
     CDS             4609..4896
                     /locus_tag="OHN27_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288555.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
BASE COUNT         1069 a         1335 c         1524 g         1008 t
ORIGIN      
        1 gatttcggga ttcttaagta agagaggtgc cggacgggga tcacgtccgg cagacataca
       61 accaccccgg tcaaaggaga aatatatgtc agttacagag tctaaggtaa aaacggagcg
      121 taaatccagc cgtaaacctg caaaaacgca ggaaacagtc ctgtcggccc tgctggcgga
      181 gacggctgaa gtgagcgtgc cgctggcctc gctgattaag tcaccgctga atgtgcgcac
      241 ggtgccgtat tctgcggagt ccgtcagcga actggcagag tccattaagg gcgtcggcct
      301 gctgcagaat ctggttgttc atgccctgcc aggtgaccgt tacggtgtcg ccgcaggcgg
      361 tcgccgactg gcagcactca acatgctggc agagcgcaac atccttccgg ctgactggcc
      421 tgtccgcgtg aaggttattc cgcaggaact ggcgactgcc gcatcaatga ctgaaaacgg
      481 tcatcgtcgg gatatgcacc ctgccgaaca gattgccgga ttccgcgcaa tggcgcagga
      541 aggcaaaaca cctgcacaaa tcggtgattt gctgggctat tcaccccgcc atgtccagcg
      601 aatgctgaaa ctggctgacc ttgcgcctgt catcctcgat gcgctggcag aagaccgcat
      661 caccaccgaa cactgtcagg cgctggcgct ggagaacgac accgagcgtc aggtacaggt
      721 gtttgaagcc gcctgccagt caggatgggg cggtaaaccg gaagtgcaga ccattcgtcg
      781 tctggtgacc gaaagtgaag tggcggtggc ggggaacagt aaattccgct tcgtgggggc
      841 tgatgccttc tcgccagatg aactgcgcac tgacttgttc agcgacgacg agggtggcta
      901 tgtggactgt gttgcgctcg atgctgccct gctggaaaaa ctccaggctg ttgctgaaca
      961 ccttcgggaa gccgagggct ggggatggtg tgccggacgc atggaggccg ttggtgagtg
     1021 ccgtgaggat gccggaacat accgcagtct gccggagccg gaagcggtgc tgacggaggc
     1081 agaagaagaa cgcctgaacg aactgatggc acgttacgac gcactggaaa accagtgtga
     1141 ggaatctgac ctgctggaag cagaaatgaa gctgatgcgc tgcatggcga aggtcagagc
     1201 gtggacgccg gagatgcgtg ccggaagcgg tgtggtggtg tcctggcgtt atggcaacgt
     1261 gtgtgtccag cgtggtgtgc agttgcgcag cgaggatgac gtggctgacg acgctgaccg
     1321 cacggaacag atgcaggaga aagcgtcagt ggaggaaatc agtctgccac tgctgacgaa
     1381 aatgtcttcc gaacgcacgc tggcagtcca ggcggcactc atgcagcaac cggacaaatc
     1441 tctggcactg ctggcatgga cgctctgcct gaatgtgttt ggcagcgggg cgtacagtag
     1501 tcccgccaga atttacctgg aatgtgggca ttactcactg accagcgctg cgccgtcagg
     1561 gaaggaaggt accgcattca tggcgctgat ggcagagcac tttcgccttg ctgccctgct
     1621 gccggatgaa tgggaacggg acatgacgac gttcctgtcc ctcagccagg aggtgctgtt
     1681 atccctgctc agtttctgca ctgcatgcag catccacggc gttcagaccc gtgagtgtgg
     1741 tcacacgtca cgcagtccgc tggactcgct ggaaaccgcc atcggctttc acatgcgcga
     1801 ctggtggcag ccgacaaaag caaacttctt cggacacctg aaaaagccgc agattatcgc
     1861 agccctgaat gaggccggat tgtccggtgc cgcccgggac gcggagaaaa tgaagaaagg
     1921 cgatgcggct gaacatgcag agcaccatat gaaagacaac cgctgggtgc ctggctggat
     1981 gtgtgcacca cgtccacaga cggataccac tgaacgcacc gataacctgg ctgatgccgc
     2041 ctgatgaata accacaccgc cccgccggag acggggcggc agcaagggag ataccgtgat
     2101 gaaaactgaa ctgaccctga atgtattaca gaccatgaac gcacaggaat atgaagatat
     2161 ccgggctgcc ggaagtgatg aacgccgtga gctgactcac gccgtgatgc gggagctgga
     2221 tgcgccggat aactggacga tgaacggcga gtacggcagc gagtttggcg gctttttccc
     2281 cgtccaggtc cgtttcacgc ctgcccacga acgttttcac ctggcgttat gttcaccggg
     2341 cgatgtctct caggtctggg tgctggttct ggtgaatgtt ggtggtgagc cgtttgcggt
     2401 ggttcaggta cagcggcggt ttgcaccgga agccgtcagc cattcactgg cactggcggc
     2461 gtcactggat acgcaggggt acagcgtcag tgacatcatc catatcctga tggcggaagg
     2521 aggtcaggca tgagcgcacg ttcacaggca ctggttcctc tcagcacaga gcaacaggcc
     2581 gcatggcggg cggtggcgga gaccgaaaaa cgccgccatc agggtaacac gctggctgaa
     2641 tatccgtatg cgggggcgtt tttccgctgt ctgaacggaa gccgcaggat atcgctgtct
     2701 gacctgcgat ttttcatgcc gtccctgacc gcagaagaac tgcacggcaa ccgtcttcag
     2761 tggctgtatg ccatcgatgt gctgattgaa acacagggcg aggtctgcct ccttcccctt
     2821 cccggtgatg cagcagagcg cttgttcccg tcggtgcggt ttcgtgtcag ggaacgcagt
     2881 cgccataaat ccgcgctggt catgcagaag tacagccgcc agcaggcgag ggaggcagag
     2941 cagaaagccc gtgcgtacca ggcgctggtc gctcaggctg aaatcgaact ggcattccat
     3001 tcgccggaaa cagtcggaag ctggcatgca cgctggagtg accgggtggc agagcatgac
     3061 ctggaaaccc tgttctggca gtggggcgag cgttttccgt cactggccgg aatggaacgc
     3121 tggcagtggc aggatatgcc gttctggcag gttattgcgg aagccagtct tgcagcaaga
     3181 gaggccggtc acgctgtccg tgagatggag cggtggatgg tgccgaacaa actccgggag
     3241 gcagcgtgat gcggcaacaa tcacacggat ttcccgtgaa cggtctgaat gagcggatta
     3301 ttttcaggga aagtgagtgt ggtcagcgtg caggtatatg ggctatgatg tgcccggcgc
     3361 ttgaggcttt ctgcctcatg acgtgaaggt ggtttgttgc cgtgttgtgt ggcagaaaga
     3421 agatagcccc gtagtaagtt aattttcatt aaccaccacg aggcatccct atgtctagtc
     3481 cacatcagga tagcctctta ccgcgctttg cgcaaggaga agaaggccat gaaactacca
     3541 cgaagttccc ttgtctggtg tgtgttgatc gtgtgtctca cactgttgat attcacttat
     3601 ctgacacgaa aatcgctgtg cgagattcgt tacagagacg gacacaggga ggtggcggct
     3661 ttcatggctt acgaatccgg taagtagcaa cctggaggcg ggcgcaggcc cgccttttca
     3721 ggactgatgc tggtctgact actgaagcgc ctttataaag gggctgctgg ttcgccggta
     3781 gcccctttct ccttgctgat gttgtacggg catgaacaac ctgacttcag gaaggtcgtt
     3841 tcccttcagg aacggggatg aacgcgcgcc tgcggcgccc gttcttttcc cccgccttct
     3901 ctggttatgg cctgtcagaa atcacatcac cttccctgtg attattctct ttttcttcct
     3961 gctctgattc tgactactgc agtgttgtcc tgtctgccgg gcgcaccttc ggctcccggc
     4021 tgcctggcgg catccggtcg tcaggcccga ctccgacagg cagctgctgg cgcacctgcc
     4081 agtctccgcc gaacccgctg gcgcggtttc gggctgtcgc tacggtccga tgcgtgaaaa
     4141 ccccgggctt gcagcgaccg ttccagtcgc gccgcctctg tcgcgccgtg gcctgcgctc
     4201 ctggcatccc ggcaaaaggc cgccgggatg taaggcgcaa cgccgtttcg caaaccgcgc
     4261 ttcacgtcgt tgcggcgggt ccctgcccgt tgttgtgtcc ggtgccattc acctgtttaa
     4321 gcccctgttg ccgggggcat agacagccac aaccgtctcc gccagtccag gggtgaaatg
     4381 cacatcccgc aaaacttttt gcccagaagc aggcaaaaac ttttgcggtc tgcccccgga
     4441 cagtcgtttc cggccgtggc agtgatgccc cttgcggcga aggttctcaa acaggtgatg
     4501 gtgaataccg gagggaacaa agagcaggtc cccgcaaaac gtcaaaaccg cggctcccga
     4561 aactcaacag atgatggggg ctgaaaacca gagagaggag aaagcaggat gagtacccga
     4621 aatatccacg ttaacaccgc gtcgtacacg cttcttgttg ccgggaagaa aaaaaacacc
     4681 ggggaagagt gggacgtcct ggaattcagc agcctgactg agctgaaaaa atatcgcaaa
     4741 agccacccgg aaaagatggc cttcagttat agctacgcgc tcagtcaggg tgtggataag
     4801 cagttccgcc atatcaacat tgcggaagcc gatcacttca aacagttcct gcgtcagatt
     4861 aagcgtgccg gcctcgatat ccgggcgatc tgctgaccgg atgtgatgaa tatctctcct
     4921 gattgttgca atcttg
//
LOCUS       DAJOSY010000189         4692 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJOSY010000189 DAJOSY010000000
VERSION     DAJOSY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.189"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..533
                     /locus_tag="OHN27_004219"
     CDS             <1..533
                     /locus_tag="OHN27_004219"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288556.1"
                     /translation="DNLTRDLLLWNGHILNSHLKGIPGAKAKHKLANIMILPEYESVR
                     ESLSAKFRDYTLLSDRFEYIKNIVSSPESIKSTLDNHKLRLEWQLRRIYRTRNNIVHS
                     GKGGKFTPLLVEHTHNYLDKVFEILVMLASKPRKIRSVTQGFRYVKIIYEQRYKTITE
                     KNFTFDMSNINNLFWD"
     gene            complement(591..1037)
                     /locus_tag="OHN27_004220"
     CDS             complement(591..1037)
                     /locus_tag="OHN27_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCQ2288557.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRI
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFAKLRTTKYLKTAASADSASVQFEGKVQRIA
                     RVHHYGLRDRVSRKGPEVRYAERRLLGVNDDLYFLTRRILLQWLAR"
     gene            complement(1030..1461)
                     /locus_tag="OHN27_004221"
     CDS             complement(1030..1461)
                     /locus_tag="OHN27_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2288558.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLNLFVDNGSLVATGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAVAEPDEPEEMWTVKRG"
     gene            complement(1557..1985)
                     /locus_tag="OHN27_004222"
     CDS             complement(1557..1985)
                     /locus_tag="OHN27_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HCQ2288559.1"
                     /translation="MTRALAVVVALALVALGWQSWRLNSASHTIETQRAALKSKAHEL
                     TKKNSQLISLSILAETNNREQARLYAEAEQTSALLRQRQRRIEELKRENEDLRRWADT
                     PLPADIIRLRERPTLTGGAAYRQWLSASDAVSAGSGNAAH"
     gene            complement(1982..2221)
                     /locus_tag="OHN27_004223"
                     /pseudo
     CDS             complement(1982..2221)
                     /locus_tag="OHN27_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069910.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
     gene            complement(2217..2354)
                     /locus_tag="OHN27_004224"
                     /pseudo
     CDS             complement(2217..2354)
                     /locus_tag="OHN27_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
     gene            2290..3189
                     /locus_tag="OHN27_004225"
     CDS             2290..3189
                     /locus_tag="OHN27_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCQ2288560.1"
                     /translation="MISACSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIV
                     TVEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEV
                     WTDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRG
                     MDFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALV
                     LKAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWAT
                     MHALFNEPLQGESANTSNIVEIF"
     gene            3189..4226
                     /locus_tag="OHN27_004226"
     CDS             3189..4226
                     /locus_tag="OHN27_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCQ2288561.1"
                     /translation="MGKSKKNRAASTKQIQHKSQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQMDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPNNVGGFGDVEKASRVFVRNELMPLQKRLQELNNWLGEKVI
                     CFESYNLDISE"
     gene            complement(4257..4553)
                     /locus_tag="OHN27_004227"
     CDS             complement(4257..4553)
                     /locus_tag="OHN27_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288562.1"
                     /translation="MKTTYSYDFQYMRKGQDRPDDDGEVVGCNSEDNSLLMLPNVGDY
                     VTITNKGGRAQFDGRVKTRLFNYLRIADGHVHCSICIVVEEVDMDSVWGALIKE"
BASE COUNT         1158 a         1271 c         1130 g         1133 t
ORIGIN      
        1 ttgacaacct tactcgagac ctgctgttat ggaatggaca catactaaac tcacatttaa
       61 aaggaatacc tggagcaaaa gcgaaacaca aactagccaa tataatgatt cttcctgaat
      121 acgaatcagt aagggaatct ttatccgcta aatttcgtga ttacacattg ctttcagatc
      181 gtttcgagta tataaaaaac atagtttcat ccccggagtc aattaaatca actttagata
      241 accataagtt aagattagaa tggcaactca gaagaattta tagaacaaga aataacatag
      301 tccattctgg aaaaggtggt aaatttaccc cccttttagt tgaacacacc cacaactatc
      361 ttgataaagt atttgaaata cttgttatgc ttgcatcaaa accccgaaaa attcgctctg
      421 taacacaggg gtttagatat gtaaaaataa tctatgaaca aaggtacaaa actattacag
      481 aaaagaactt cactttcgat atgagcaata ttaataattt attttgggat tgatataacc
      541 taaaatcaat cggtgaggtt agagtaggaa ctacaaaaag tcaaccaact ctacctcgcc
      601 aaccattgca ataaaatgcg tcgtgttaaa aagtataaat catcatttac acccaacagg
      661 cgacgctctg cgtaacggac ctccggtcct ttgcgactga cgcgatcacg gagaccgtaa
      721 tggtgaacac gggcaatacg ctgtacctta ccttcaaact gtacgctggc agagtcggcg
      781 ctggcggcag ttttcaagta ttttgtggtg cgcagctttg caaacatctg acgtttgatg
      841 cgccccttct tgctgcgtgc tgttaccctg cgcggttcat aactgctgcc atctggattg
      901 cgctgtatcc tgatattctg ctgctgtgtc cggcgcagtt cctgcgccag ctggcgcatc
      961 atgcggcttc ttgcggctgg ttccagattc gccagtaagg cactcagcca gtcgtccacc
     1021 ttctgcagtt cagccacgtt tcaccgtcca catttcttca ggttcatcgg gttccgctac
     1081 cgcttcaaca ctcgacacac tgccgtcagt gctgaccagc acacgctccg tcagttgcag
     1141 gttgaggctg atatcacaga catcgttgcg cagaatatcc acctcaaagg tgaatagttt
     1201 ttcccgtaac gccgggttat tgatggcatc gggctggtta tccctcagcc acagcaaaac
     1261 cggggccatc agcagattct ggtcgccgct gaaatcctct atcaccacgt tcagggtata
     1321 gcggtactcc catgacatgg agctggcccc cgtagcaacc aacgaaccgt tatccacaaa
     1381 cagattcagt ttgtccgggt tattgcggac ataaggcacc gctttattga gggcgtggcg
     1441 cagggattgt ggtttgttca ctgtttcgct cctgacacgc aataataatg tccactttgt
     1501 ctgcacagac cgcccaggcg gcctccgttt catccagcaa cgcgttcaga tcaccgttag
     1561 tgcgcggcgt tgcctgatcc agccgacacg gcgtcactcg cggacaacca ctgacggtaa
     1621 gctgcacctc cggtgagtgt cggacgttcc cgcagccgga taatgtcagc aggcaaagga
     1681 gtatcagccc agcggcgtaa atcctcgttc tcacgtttca gttcctcaat ccggcgttgt
     1741 cgttgtctca gcagtgcact ggtctgttct gcttcggcgt agagccgcgc ctgctcccgg
     1801 ttgttggttt cagccagaat ggacagactg atcagctggc tatttttctt cgttagttcg
     1861 tgcgctttac ttttcagcgc cgcgcgctgc gtttcgatgg tgtggctggc gctgttaagc
     1921 cgccacgact gccagcccag cgcaacgagt gccagcgcca ccactactgc cagcgcacgt
     1981 gtcatagtcc agctccttta aggcaccagg ccatctcccg cgcacggcgg ttatccagcc
     2041 cctgattaaa aacacctttt acatacaccc agcgcggcaa ctgtcggcac gcatctgccc
     2101 agcgccgctg attgagtaat ttcaccagtg tggaactgca ggcattgccc gttcccacgt
     2161 tgaaggcaaa cgacaccacc gagtcataca ccttctgcgg cggctgttgc ttcacacacc
     2221 gtgccatcag tgcgcatcac actgcgaaac tccgacagtt ccacatcagg ccagaacggc
     2281 gtatttctga tgatttccgc ctgttccggt gcgctgttca accgtgggcg caacaaagcc
     2341 gataaggtgg acatcgacct gtcccacagc aatctggccc ccggcctgct gtgcgcagac
     2401 gggcaatacc gccagatagt caccgtggaa gatgcggtgc gcggcggctg taacctgttc
     2461 gaccttgacc agttgcgcat ggagtacagc ccggacgaat accagaacct gctgatgtgc
     2521 gagtttgtgg acgatctcgc gtccgtgttt ccgctcagcg agctgcaggc gtgcatggtg
     2581 gacagctggg aagtctggac cgactttcat gcactggcgc tgcgcccgtt tggctggcgc
     2641 gaggtgtgga tcggttatga cccggcaaaa ggtacgcaga acggcgatag tgccggatgc
     2701 gtggtggtgg ctccgccagc cgtgccgggt ggtaagttcc gcattcttga gcgtcaccag
     2761 tggcgcggga tggacttccg cgcccaggct gacgccatca aaaaactgac cgaacagtac
     2821 aacgtgacct atatcggcat cgactcgacc ggcgtcggtc acggggttta cgagaacgtg
     2881 aaagcgtttt ttcctgccgt cagggagttt gtctacaacc ccaacgttaa aaacgccctg
     2941 gtactcaagg cctacgacat tatcagccac cgccgtctgg agtttgacgc cgggcacacc
     3001 gacattgcgc agtcatttat ggcaatccgt cgcgctacca ctgccagtgg caaccgcccg
     3061 acctatgaag ccagccgcag cgaagaagcc agccatgccg atctggcctg ggcaacaatg
     3121 cacgcactgt ttaacgaacc gctgcagggc gagtccgcca ataccagcaa tattgtggag
     3181 attttttgat gggaaagagt aagaagaacc gcgctgcgtc gacgaaacag atccagcata
     3241 aaagccagac ttcagccgaa gcattcagct tcggtgatcc cgttcctgtt ctggaccgcc
     3301 gcgaactgct ggactatgtg gaatgcgtac agatggatcg ttggtatgag ccgcctgtga
     3361 gtttcgacgg actggcgcga accttccgcg ccgccgtgca tcacagctca ccgattgcag
     3421 tgaagtgcaa cattctgacc agcacctaca tccctcaccc gctgctcagc cagcaggctt
     3481 tttcgcgttt tgtgcaggac tatctggttt ttggtaacgc ctacctggag aaacgcacga
     3541 accgcttcgg tgaagttatc gcccttgagc ctgcactggc aaaatacacc cgacgcgggt
     3601 tagacctgga tacctactgg tttgtgcaat acggtatgac aacccagccg tatcagttca
     3661 cgaaaggcag tatttttcat ctgatggaac cggacattaa ccaggagatc tacggcctgc
     3721 ccggttatct ttctgccatt ccgtcagctc tgctcaacga gtccgccacg ctgttccgcc
     3781 gcaagtatta cattaacggc agtcatgcag gcttcatcat gtacatgacc gatgctgcgc
     3841 agaaccagga ggatgtgaac aacctccgca atgcgatgaa aagcgccaaa ggtcctggta
     3901 acttccgtaa cctgtttatg tactcgccta acggtaaaaa ggacgggctt cagattatcc
     3961 cgttgtcaga agtggcggcg aaggatgagt tcctgaatat caagaacgtg agccgggacg
     4021 acatgatggc ggcgcatcgt gtgccgccac aaatgatggg gataatgcct aataatgtcg
     4081 gggggtttgg ggatgtggag aaggctagtc gagtgtttgt ccgcaatgaa ctgatgccac
     4141 tgcaaaagcg gctgcaagag ctgaacaact ggctaggtga aaaagtgatt tgctttgaat
     4201 cttacaactt agatatttct gagtaaatta aagcgcctgc atttgcaggc attctattat
     4261 tctttaatta gtgcccccca tacggagtcc atatcaactt cctcaactac aatgcaaatg
     4321 gagcaatgaa catgaccatc agcaatgcgt aggtaattga aaagccttgt tttcacccgc
     4381 ccatcaaatt gagcacgccc acccttattc gtaattgtta cgtagtctcc cacatttgga
     4441 agcatcaaaa gcgagttgtc ttctgaatta caccctacaa cctctccatc atcatcaggg
     4501 cgatcctgac ctttgcgcat atattgaaaa tcatagctat aagtagtttt cataaatgtc
     4561 ttactattcc aatttttaac taacccagaa gttatatctt agacctaaac tgtctcagtc
     4621 accgttaact tcatcacaac gatttaacac atcctgcatg cctatctaac gtgagagccg
     4681 tttataccta ca
//
LOCUS       DAJOSY010000190         4610 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJOSY010000190 DAJOSY010000000
VERSION     DAJOSY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.190"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            276..482
                     /locus_tag="OHN27_004228"
     CDS             276..482
                     /locus_tag="OHN27_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288563.1"
                     /translation="MHKDQYIDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
     gene            complement(733..927)
                     /locus_tag="OHN27_004229"
     CDS             complement(733..927)
                     /locus_tag="OHN27_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCQ2288564.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            1316..1861
                     /locus_tag="OHN27_004230"
     CDS             1316..1861
                     /locus_tag="OHN27_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCQ2288565.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSTEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1836..2885
                     /locus_tag="OHN27_004231"
                     /pseudo
     CDS             1836..2885
                     /locus_tag="OHN27_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            3260..3835
                     /locus_tag="OHN27_004232"
     CDS             3260..3835
                     /locus_tag="OHN27_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCQ2288566.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEEIKNNLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTSQDIEWPALP"
     gene            complement(3933..4523)
                     /locus_tag="OHN27_004233"
     CDS             complement(3933..4523)
                     /locus_tag="OHN27_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2288567.1"
                     /translation="MSQIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT         1192 a         1012 c         1155 g         1251 t
ORIGIN      
        1 caatttctca tcagatgcta ttcaacaata caaattaccc ataaacctcg tttttacggg
       61 gttttgttat atttaaacat taccgaatag atataaatct cgattgtcga tggtattggt
      121 tgtgtgacaa tacctagtct ttccggtatg cctgaagaga atacaaacga cagattatgt
      181 aagggaaacg cgctgaaact ttcactcttt catgctgagt gatttgacgt tatattttta
      241 gccgcaagtg aagagcaaac gaatggagcg acaaaatgca taaagatcaa tacattgatg
      301 ccccctccca ggagcaggtt cgtgtaaaaa ctatgcttaa tagcaccatt tcgatgggtt
      361 acccggatgt tgtaattgca tgtatagaac atcaagtatc tctggaagca ttcagggcaa
      421 ttgaggccgc gttagtaaag cacgataaga atacgaagga ttattccctg gtggttgact
      481 gatcaccata actgctaacc attctgacca tttcacaagt gacagagtca atatcgtatt
      541 ctgtcactgt caggctaata cagtactgca attcaactac agcaatgcct cgtatttagg
      601 tgaatttaca atatcgttct attcggatac caactacatt tctgaagata aggcatttat
      661 ggctcacata ttggtccctc acgctcgcca gttctgtgcg gggagggagg gaaggtcaag
      721 aaaccgggat gttcatccat catcactctg gattgatgta tatgctctct tttctgacgt
      781 tagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgatc
      841 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
      901 tctgcgggtt ctgttcttcg ttgacatgag gttgccctgt attcagtgtc gctgatttgt
      961 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
     1021 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1081 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggcgacctcg
     1141 cgggttttcg ctatttatga aaattttccg gtttaaggca tttccgttct tcttcgtcgt
     1201 aacttaatgt ttttatttaa aataccccct gaaaagaaag gaaacgacag gtgctgaaaa
     1261 cgagcttttg ggcctctgtc gtttcctttc tctgtttttg gccgtggaat gaacaatgga
     1321 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     1381 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     1441 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     1501 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     1561 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     1621 cagagactcc actgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     1681 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     1741 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     1801 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     1861 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     1921 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     1981 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     2041 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     2101 tttatgccta cttcatagag cataagcagc gcaacaccct tatctggttg ccgacggatg
     2161 gtgatgccga gaactttatg aaaacccacg ttgagccgac tattcgtgat attccgtcgc
     2221 tgctggcgct ggccccgtgg tatggcaaaa agcaccggga taacacgctc accatgaagc
     2281 gtttcactaa tgggcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     2341 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ttttgatgat gatattgaac
     2401 aggaaggctc tccgacgttc ctgggtgaca agcgtattga aggctcggtc tggccaaagt
     2461 ccatccgtgg ctccacgccc aaagtgagag gcacctgtca gattgagcgt gcagccagtg
     2521 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtacc
     2581 ttaaatttgg cgataaagag acgccgtttg gcctcaaatg gacgccggat gatccctcca
     2641 gcgtgtttta tctctgcgaa cataatgcct gcgtcatccg ccagcaggag ctggacttca
     2701 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     2761 tttcgtcatc cggtgaagag attgaaccgc ctgacagcgt gacctttcac atctggacgg
     2821 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     2881 atacccatag tttaagtggc agcacgaatg cagctggtaa tcacagccat agagatggcc
     2941 gtcgatttaa ccccagtgtt tttaaagata cttatcaata tggttataca agctcaggtc
     3001 aaaatacctg gggtgtacaa ggctcagtag gtatgtctac ggggtggtta gctaatacca
     3061 gtacagatgg taatcatagc cactcactgt ccggcacagc agcatctgca ggtgcacacg
     3121 cgcatactgt cggtattggt gctcatacgc actccgttgc gattggttca catggacaca
     3181 ccatcaccgt taacgctgct ggtaacgcgg aaaacaccgt caaaaacatc gcatttaact
     3241 atattgtgag gcttgcataa tggcattcag aatgagtgaa caaccacgga ccataaaaat
     3301 ttataatctg ctggccggaa ctaatgaatt tattggtgaa ggtgatgcat atattccgcc
     3361 tcatacaggt ctgccagcaa acagtaccga tattgcaccg ccagatattc cggcaggctt
     3421 cgtggctgtt ttcaacagtg atgagtcatc gtggcatctc gttgaagatc atcggggtaa
     3481 aacggtttat gacgtggctt ccggcgacgc gttatttatt tctgaactcg gtccgttacc
     3541 ggaaaatgtt acctggttat cgccggaagg agagtttcag aagtggaacg gcacagcctg
     3601 ggtgaaggat acggaagcag aaaaactgtt ccggatccgg gaggcggaag aaataaaaaa
     3661 caacctgatg caggtagcca gtgagcatat tgcgccgctt caggatgctg cagatctgga
     3721 aattgcaacg gaggaagaaa tctcgttgct ggaagcatgg aaaaagtatc gggtattgct
     3781 gaaccgtgtt gatacgtcaa cttcacagga tattgaatgg ccagcactgc cgtagggtaa
     3841 aacatataaa ttctataatt agatgtatct ttccatttac ggcaaggaag ggggcttgga
     3901 agacgtaaag catctcacac cgagattatt ttttatatgt caggtgtctg aagttttgct
     3961 ttggctctta aaatggtttg ccgcgaggtt ttgaattccc gggcaatggc acttatactt
     4021 acacctgact taattcgttc gaataccacc tgtttctgtt cttcatttaa cacaggtggt
     4081 cgaccaaaac gtttccctgc gccgcgggct cttactatcc cggaatgagt gcgttcaagt
     4141 aaaaggtctc gttcaaattc agcgactgct gaaattactt gcatcatcat ttttcctgtt
     4201 ggactggtca ggtcaatgcc ccccaatgct aagcaatgca ctctgatacc tgtttcggtc
     4261 agttgttcca ctgttttcct gatatccatt gcattacaac caaggcgatc cagttttgtc
     4321 acaatcaatt gatcaccaca tttcaggcga gcaagcaacc ggttaaaacc aggacgctca
     4381 ctggttgctg ctgagccgct aatgtgttct tcgattattt gctgaggttt gattttaaaa
     4441 cctgcacttt cgatttcccg gcgttgattt tcggtggtct gatccagcgt tgatatccga
     4501 cagtaagcaa aaatttgaga catagtgaga ctctatacga aattggtgtt catatcataa
     4561 tgcatctcag aaaataatta tgattatttt tgtgcatatt tgtatgtaca
//
LOCUS       DAJOSY010000191         4571 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJOSY010000191 DAJOSY010000000
VERSION     DAJOSY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.191"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..308)
                     /locus_tag="OHN27_004234"
     CDS             complement(<1..308)
                     /locus_tag="OHN27_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HCQ2288568.1"
                     /translation="MFISLMTEQTEATEIKMNMRNINIITARSVPDKSVSDDFMNAVL
                     SNCTTRIVLPALKEFGSESLPHNFNMAAVGVMKKGMSMSLFSSPGHIVVAGQSGGGKS
                     V"
     gene            complement(247..837)
                     /locus_tag="OHN27_004235"
     CDS             complement(247..837)
                     /locus_tag="OHN27_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HCQ2288569.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAAEYARETDQSLMQLFTALWATLALMYVSLAVISRKYPMRPGKMKCVRVATADEHLK
                     EVLTEDASLPEKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(827..2254)
                     /locus_tag="OHN27_004236"
     CDS             complement(827..2254)
                     /locus_tag="OHN27_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HCQ2288570.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGSNDGK"
     gene            complement(2254..2958)
                     /gene="traK"
                     /locus_tag="OHN27_004237"
     CDS             complement(2254..2958)
                     /gene="traK"
                     /locus_tag="OHN27_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HCQ2288571.1"
                     /translation="MFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLK
                     APAGLSSVADAVWMGNHLKMVRFTVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            complement(2969..3535)
                     /gene="traE"
                     /locus_tag="OHN27_004238"
     CDS             complement(2969..3535)
                     /gene="traE"
                     /locus_tag="OHN27_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HCQ2288572.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(3557..3868)
                     /gene="traL"
                     /locus_tag="OHN27_004239"
     CDS             complement(3557..3868)
                     /gene="traL"
                     /locus_tag="OHN27_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCQ2288573.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(3883..4242)
                     /gene="traA"
                     /locus_tag="OHN27_004240"
     CDS             complement(3883..4242)
                     /gene="traA"
                     /locus_tag="OHN27_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HCQ2288574.1"
                     /translation="MDAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGL"
     gene            complement(4276..4503)
                     /gene="traY"
                     /locus_tag="OHN27_004241"
     CDS             complement(4276..4503)
                     /gene="traY"
                     /locus_tag="OHN27_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HCQ2288575.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVAEESESTFKEL"
BASE COUNT         1088 a         1277 c         1079 g         1127 t
ORIGIN      
        1 acagattttc cgccaccgga ttgtccggca accacaatat gccccggaga agaaaataaa
       61 ctcatcgaca tacccttctt catcacaccg acagctgcca tattaaaatt gtgcggcaat
      121 gattcagaac cgaattcctt cagtgcagga agtacgattc ttgtggtgca gttactgaga
      181 acagcattca taaaatcatc tgatacgctt ttatccggta cagagcgtgc agtaataata
      241 ttaatattac gcatgttcat ttttatctcc gttgcttctg tttgttcggt catcagggaa
      301 ataaacatgg catttcctga ttttttccgg cagggaagca tcttccgtaa gaacttcctt
      361 cagatgttca tctgcagttg cgacccggac acatttcatt tttccgggtc gcatcggata
      421 ttttctggag atcaccgcca gcgagacata catcagtgcc agcgttgccc ataatgcggt
      481 gaacagttgc atcagagact ggtcagtctc cctagcatat tctgcagcat cagccgcgcg
      541 gtaatggcag atatcctgta tttctgatct gcctgaagaa tcagaggaag aaaaccagca
      601 ttcactgacc gggaactgtc cggcaggtgc tgtttgtctt acgaagttaa tctccttaac
      661 cagtaatttc cccggcgtgg tgttgtcctt ccataaaaca aacagagcca tcagggccat
      721 tgtggccagt ggcagaatga cggtgtactt cagacaccag aacagtcctc gcagaacccg
      781 ggcgacaaca taccgggcgg catggcacgc ctgacgggac gacatattat ttgccatcgt
      841 tgctccctcc ctggctgccg gacataaact gctgcggatt caggttgccc agctgcttca
      901 gcatctggtc agtattaaag ccgttaagat gactttcagc cgatggcgga acaggaaccg
      961 gactttccgg attgtcttct tccgctctgc tctgcgtgcg ttccagcgcc atctcttcca
     1021 cggttttcag ctggaagccg tcctggaaca ccacggtcac ttcgttgccc gcaccaatcg
     1081 gtatcaccgg gtgatactgt tcggcacgtt tgatgtagta gtcactgagc gtctgtgcgg
     1141 ctttcgatgc gccgccaccg atacccattt tcaggacatc accagccccg taagcggctg
     1201 tggcacccag cccgacagcc ggctgggagg cgcgctccat tccctgaccg ataccatcaa
     1261 caaatcccgc gccccatgcc cagccgagga ttttgccgtt acgcatcacc acttcgccct
     1321 tgataccgtt tttaccccgg aagctgacat gccccttaat cggcatatca attgttttgc
     1381 cgtccttcag gcagctgata ttgcgggtac gtacaatggc acgctcactg gagacatccc
     1441 cccaggcttc cagacccaca aaacatcccg ttgcgtcata cgtcttgctg ttcggcattt
     1501 ccaccaggcc ggtgatacgc aactgcatcg gcaccgtgga ttcattaccg gtgactgagg
     1561 cattggcatc agccccttca atcagcatgg ctttcgcaaa acttcctgac ggaatgtacg
     1621 gcagtgatgg tccctgtttt ccctcattac gggtgaacac cttacgctgt atccggttag
     1681 gcaccggtac agactggtac gtcacctgcg gtggtggcgt gacaccattc ccgggataaa
     1741 acgccgtcgg cggtggaaca gcaacgctgc cctgcggcgg gaaagatacg ggtgtgtttc
     1801 ctggctgtgg ttcgccttcc gggccgggcg gtgaggctgg cgtctgcggt actggctcac
     1861 cggtcgcggt gacaggattt gcacccaggg ctttcacctg ctctgccagt gcagcattgt
     1921 cctgtcccag cttttcaata cgacgctggt catcaccgcg ctgtttgttc aggacatcca
     1981 gctcacgacg gatttcctca tactgcttct gcatctgtgc tgccgtcacc tgcatctctg
     2041 tggtggcatg ctgacgcacc ttgtcatcaa aggtcgtatc caccacaccg gtcatatccg
     2101 gcacaggctc ctgttcagcc acggtttcac cgttaccgga catgtccaca tcagacagat
     2161 acagtgcccc accaatcgcg gaggctgcac cgacaaccac aatccccagc cacaggtact
     2221 gcttgcgttt cacaatggta ttgatactgg ccatcagttg ccctccccgt cacggatgac
     2281 atacacatcc atgcgcgcac ctgccagtaa ctggctggca ggctggctga acatcacggc
     2341 acgggttccc ggctgccaga aatcactttc ccggatattc agggcagaca gcgttttatt
     2401 ttccacggta aagcggacca tcttcaggtg attccccatc catactgcat ctgccactga
     2461 agacagaccc gcaggggcct tcagggtttc ctttgtcaca ggaacctgat accagccagc
     2521 gggtaatttt ccgccacgga cggcctggct gatggtcaca agcagggatt cgtaaggcgt
     2581 ggacgtttcc cacgcaccgg cttcttctcc ggttccgcgc aggtcactga ccagctgaat
     2641 ggtacgcccc gcgccttcac ggggaacggc ctgaatggaa agattcagac cacgttctgt
     2701 ttccagaatg aacgtaaagg gctttttgtt gacggtggcc accaccacac cgccggaggc
     2761 ggtctgctca ttattggtca gggcaccatc cagactgttc acggcgataa tccggtcacc
     2821 gggaacggta aacagattcg ggctggtatt gctgatggca atactggcct gaccaccgtt
     2881 caccattggc accacggtgg gggccagcgt accgtttgcg gccatcacgc tgcaggaaaa
     2941 aaacaggctg ccgaatatta ttgccgtatt attttttctc atcgtctgtt tccccgaaat
     3001 tatccagcca ggtcaccccg ttttcccgct tcagaataag gatgtaatgt ttgatatcag
     3061 tgaaaggttt tgaatcacca atccaggttt taagcacacc tcgaatttcc acacggccat
     3121 actgaggcca gacacgaaca ctggtctgga aaaaggctga gttcacgtta tcgtttttaa
     3181 tacgcttcgc ttcttcagcc agaataactt tcatctggtt ctgtgcgccc gggcggatat
     3241 attgcagaag cgcctgatgt gaggcatcga cggtttctga tgaaacattc agacggaggg
     3301 caataaatga caacgccatc tgctgtaaat aagaggcatc ggcactgttc tgcgacacgg
     3361 caaagggggc attaaatgcc attggcgtca cggcagtgcg ttgctcattc tgaagacggt
     3421 agttattcac cccctgaatg acgttaacag agaggctgag aacaataagc actgacataa
     3481 atataaaggc gatggccatt acacgactgg tacttaaacg ggcaccgtgt tccatataac
     3541 aatcctggta tcagttctat ttaatccact gccggaaaca cgaatcggga acattatgaa
     3601 aaataccgcg cagcagggct gttggcatat accagtaaat caggtcacgt aaccaggaac
     3661 tgccccgccc ttttttcagt tttttaatcc cgaaataaac cagaaccgct gcaccaatac
     3721 cgaacagata tttcgatgtt gtgatacccc agccaataca gattgctgcg gggatcagtt
     3781 catccagtgg caggccaaac cagcggctct ggttggtcag tgtttccggg aaacgatatt
     3841 tcttaagttt attctcgtct cccgacatcg ttttatttcc tgtcagaggc caacgacggc
     3901 catacccaca gcaataaata cagagatgat ggcaaaaccg gccaggaact tgacgttttt
     3961 ggtcatcatg tacatgactg caccgaccag aacttcagcc agaacaaccc atttaacaac
     4021 actggagtcc ttaccgaagg tcgccttaac cgtggtgtta ccgcttgcca tcaagtcctg
     4081 gccctgtgct gccattgcca gttgcgggaa gaacatcatc agaacagcag ccgggatcac
     4141 agcgcgagcc aggcgaagca tattcagacg agtgaattta gaaaaaaatg acttcttttt
     4201 gacgggcgca gaagcaccct gaacacttaa aacggcgtcc atatgattcc tctgtttaat
     4261 attgatagat aaaaactaaa gctctttaaa tgttgactcg ctctcttcgg ccacctccct
     4321 gaatatttct tcgttataga aatcaggaaa ccgtttcaga tgatctctga gacggatctg
     4381 gacttctatc gttttacttc ttccactacg atctttggcc ttaataagtc ggttgtttgt
     4441 atcttcatcg agatacaccg agaccgttct gcttataccg cctctcgcgt tacgcctcct
     4501 cactaaattc acctctgttt cccgactaat aaaattatga ttgcacagaa accggcgaaa
     4561 aaaaacacgc c
//
LOCUS       DAJOSY010000192         4497 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJOSY010000192 DAJOSY010000000
VERSION     DAJOSY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.192"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            640..870
                     /locus_tag="OHN27_004242"
     CDS             640..870
                     /locus_tag="OHN27_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288576.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            922..2283
                     /locus_tag="OHN27_004243"
     CDS             922..2283
                     /locus_tag="OHN27_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HCQ2288577.1"
                     /translation="MTSAAITMTTPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQRQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            2331..2894
                     /locus_tag="OHN27_004244"
     CDS             2331..2894
                     /locus_tag="OHN27_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCQ2288578.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRDNFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            3514..3720
                     /locus_tag="OHN27_004245"
     CDS             3514..3720
                     /locus_tag="OHN27_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCQ2288579.1"
                     /translation="MHIPQNFLPRSRQKLLRSAPGQSFPAVAVMPLAAKVLKQVMVNT
                     GGNKEQVPAKRQNRGSRNSTDDGG"
     gene            3746..4285
                     /locus_tag="OHN27_004246"
     CDS             3746..4285
                     /locus_tag="OHN27_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCQ2288580.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQAEAGTKKGGAKTKGRGRKAAQPE
                     PQPQLPEGDDYGFSDDIPF"
     gene            4347..>4497
                     /locus_tag="OHN27_004247"
     CDS             4347..>4497
                     /locus_tag="OHN27_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCQ2288581.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAVQYRNVFIEDDHGEQFRLV
                     VRNNGA"
BASE COUNT         1012 a         1271 c         1344 g          870 t
ORIGIN      
        1 tcgcgccgcc tctgtcgcgc cgtggcctgc gctcccggca tcccggcaaa aggccgccgg
       61 gatgtaaggc gcaacgccgt ttcgcgcacg cgctctccgt cgttgcggcg ggtccctgct
      121 cattgcctgc cgggtgccac acacccgtat gagtccctgt cgccgggggc tgatacagcc
      181 acaacctgcc gcactgactg tccagggtaa atgaacagcc cacaaaattt tttacccaaa
      241 acccggtaaa aaattttgcg ttctgcccag gacaggtgcg tcaggccgtg gcagtgatgc
      301 cccttgcggc gaaggttctc aaacgggtga agttcatacc acagggaata cagcagcagg
      361 ttcccgcgaa acgtcaaaac cgcggctccc gaaactcaac gggtgatgtt ctttaaaaat
      421 caggatttat ttcaggctcc ggcaatacgc atttatttgt tctgtcagtc atccggctgg
      481 caggtaatga gaacgcagga acagaaggag gaaaacaccg gaaagcgtca gatgcagata
      541 tgaaaaaagc aggtcaggga cctgcttaat ccacgccgta gaaatcgccg caatcgattt
      601 tcacggcttc actaacctgt gtcataagga gtttcattta tgacagtaag tattgtttca
      661 ccttccgcag cggctgtaaa gccccgccgt catccccgtt tcagacgcga agacatcccc
      721 gcgccggaaa ttgaccccgt gctgaaagca ttcgggcgac acatagcccg gagttttcac
      781 cggggacgtg gtgtacacat ccccgccatg aaaaacacgg ctttcgggca ggtgctccgc
      841 acacttgaac tgaaacgcgc ttttaactga tgaggtgagc cgccccgcga ggggcggcaa
      901 caaacggaag gagaacattt tatgacatca gcagcaatca ccatgaccac cccggaagcc
      961 gccagccctg tgcagatgta ccgcgcgacc tactcaccgg atgacaacaa actgcgcctg
     1021 tatgccgtgt cacgtcttga cccggagacg tataaaaaag tgcatgatgc cggttttcgc
     1081 tgggcaccaa aacaggcgct gtttgtcgcg ccagcctgga caccgggccg ggaagacgtg
     1141 ctcctctcac ttgccggaga gattgaggat gaagacagca cgctcgctga acgtcaggaa
     1201 gcacgggcgg agcggtttac cggatacagc ggaaagcggg ccagtgaatc cgcacaggca
     1261 cttgatgaag tggaaagact ggccgcgatg atcccgcccg gtcagcccat tcttgtgggg
     1321 catcacagcg aacgccgcgc ccgtcgtgat gcgcagcgta ttgaaaacgg catgaaacga
     1381 gccgtgatgc tctttgaacg tgcggaatac tgggaggagc gggcgcggtc ggcactgctt
     1441 cacgcgaagt ataaagaacg tccggacgtt cgctggcgtc gtatcaaaaa aatcgaagct
     1501 gatttgcgca aggctgaaaa gaccatcgcg cagtcgcaga aatatctgac gatgtggcgg
     1561 gctgaatcgc tggatctgaa tatggcaaaa ctcatcagca gtcatgacca tatcagcgcc
     1621 tgtttcccgc tggatacgta tccgcgcccg gcagaaaaaa gccagtatga aggaagtcga
     1681 tcgttatggt cggccctgga tgatgacatc atcaccacgg agcaggcccg cgaaattgcg
     1741 atccgctgtc atgaacggca gatccagcgt cagcaacgct gggttaacca ctatcagaac
     1801 cgcctgatct atgagcgtgc catgctggac gaaagcggcg gcgtggttac ccggacacag
     1861 gattttgagc cgggcggaca ggttttcagc cggggcgagt ggctgaccat catccgtgtg
     1921 aacaaaagca acggggcggt gagttcagtc acaacgccaa attacagttt tctcgggtac
     1981 agcggcacga tgaaagtgac gcccgatcgc atcacggact acaaagcacc atcggcagaa
     2041 gaggctgccg tcgccagtca ggccgcgaag cgtccgccgg tagtcaacta tccgggggaa
     2101 ggtttccggg aaatgacaaa ggcacagtgg gccgccctgc cccgggactg taaggccgtg
     2161 cgcagtgtgg cagaagcaga agaccacggg gcataccgct accgccgcac aatggacaat
     2221 aatttccgtc tggtgaatgt gtatatcacc gacatgaaaa tcacggaaat tccacagaaa
     2281 taaggtatat ccccccggga aaatccccgg gggatgacgg agataatgac atgcacagtc
     2341 agttaagaga acgtatccgg ctgatgcgcg caaggctgga taacgccgcg ccggttgctg
     2401 aaatccgggc tgaatctcag ctttttgtga ctcctgcccc ggtctgtgat cgcctggtga
     2461 cgctggcgga gatcagcaac cgtgatcaca ttctggaacc ctctgccggc accggggcca
     2521 tactgcgggc gattcgggat accgcgccgg gggccatgtg tgatgcggtg gaaatcaaca
     2581 gcgggctggt ccggtatctg cgggataatt ttaacggtgt cagggttcag tgtggcgatt
     2641 tcatggaatg gcagccggtg cagtattaca gccgggttat catgaatccg ccgttcagcc
     2701 acgggcagga tatccggcat atcctgcggg ccttttccct gttgcgtccg ggtggcgtgc
     2761 tggttgccgt ctgtctgaac gggccgcgcc agcaggagaa gctgttaccg ttttctgacg
     2821 tccgcgagga gctgccacgc ggtacgtttg cttataccga tgtcccgacg atgattattc
     2881 gtctgcgtgc ctgaagacgc cgggcaggcg ggaggataaa tgcccgtctg cccgcgccgg
     2941 gaaaagaccg cgcgccggtg gcgctgaaag gggccgcgct cacgcgccgc ccctttcatt
     3001 acgcggacgt tctgatgggg gcttctgttt cttcatgcct gtcatttgtc tgttactgat
     3061 gttgttgttc ctgctactgt tcttcttttt cctgctgctc tgattctgac tactgcagtg
     3121 ttgtcccatc tgccgggcgc accttcggct cccggctgcc tggcggcatc cggtcgtcag
     3181 gcccgactcc gacaggcagc tgctggcgca cctgccagtc tccgccgaac ccgctggcgc
     3241 ggtttcgggc tgtcgctacg gtccgatgcg tgaaaacccc gggcttgcag cgaccgttcc
     3301 ggtcgcgccg cctctgtcgc gctgtggcct gcgctcctgg catcccggca aaaggccgcc
     3361 gggatgtaag gcgcaacgcc gtttcgcaaa ccgcgcttca cgtcgttgcg gcgggtccct
     3421 gcccgttgtt gtgtccggtg ccattcacct gtttaagccc ctgtcgccgg gggcatagac
     3481 agccacaacc gtctccgcca gtccaggggt gaaatgcaca tcccgcaaaa ctttttgccc
     3541 agaagcaggc aaaaactttt gcggtctgcc cccggacagt cgtttccggc cgtggcagtg
     3601 atgccccttg cggcgaaggt tctcaaacag gtgatggtga ataccggagg gaacaaagag
     3661 caggtccccg caaaacgtca aaaccgcggc tcccgaaact caacagatga tgggggctga
     3721 aaacatcaac agaaggagac acatcatggc agttcgtgga attaacaagg tcattcttgt
     3781 cggtcgtctg ggaaaagatc cggaagtccg ttacatcccc aacgggggcg cagtggcaaa
     3841 cctgcaggtg gccacgtcag aaagctggcg tgacaaacag acgggggaga tgcgggagca
     3901 gacagaatgg catcgcgtgg tgctgttcgg caagctcgcg gaagtggcag gtgaatatct
     3961 gcgcaagggc gcgcaggtct acatcgaggg gcaactccga acccgtagct gggaagataa
     4021 cggtatcacc cgttacgtca ccgaaattct tgttaagacc acgggcacca tgcagatgct
     4081 gggacgtgcc gcaggtgctc agactcagcc ggaagagggg caacagttca gcggtcagcc
     4141 tcagccggaa ccacaggcgg aggccggtac gaaaaaaggt ggcgcaaaaa cgaaaggccg
     4201 tggacgtaag gccgcgcagc cggagcctca gccgcaactg ccggagggtg acgattacgg
     4261 gttttcagac gatatcccgt tctgaacgac tgactgtgac aacggccccg ccccgtcctg
     4321 tacggggcat caccggagag atgaggatga gcgaatattt cagaatactt caggggctgc
     4381 ctgacggctc ctttacccgc gaacaggccg aagccgttgc cgtacagtac cggaacgtct
     4441 ttatcgagga tgatcacggc gaacagtttc gcctggttgt ccgtaataac ggtgcaa
//
LOCUS       DAJOSY010000193         4486 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJOSY010000193 DAJOSY010000000
VERSION     DAJOSY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.193"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(433..585)
                     /locus_tag="OHN27_004248"
     CDS             complement(433..585)
                     /locus_tag="OHN27_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCQ2288582.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            877..2085
                     /gene="trbA"
                     /locus_tag="OHN27_004249"
     CDS             877..2085
                     /gene="trbA"
                     /locus_tag="OHN27_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HCQ2288583.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPYIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            2104..3174
                     /gene="trbB"
                     /locus_tag="OHN27_004250"
     CDS             2104..3174
                     /gene="trbB"
                     /locus_tag="OHN27_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HCQ2288584.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            3167..>4486
                     /locus_tag="OHN27_004251"
     CDS             3167..>4486
                     /locus_tag="OHN27_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HCQ2288585.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLC"
BASE COUNT         1154 a         1151 c         1090 g         1091 t
ORIGIN      
        1 ggggaaactc atgctttgaa tcagaaccaa ccatctcaac tttgccgtct cctgaaaaaa
       61 ctggcacatt cagttcgata actacccata aatacagaga gaaaatccga tgtcagaaaa
      121 gtggagtaaa gaattcgact cttgctttca ggtggggagc atagaataaa ttgccgatgc
      181 tctcgagaaa cgcagcggaa gcgaggaaaa tgcgttagcc atgctggcaa tgacagcctt
      241 cacactcatg acccgacgtg gtatctgcga aatgcagaac gtggccccgg atggcaaagg
      301 cattcggctg gagctaatcg gcttcagggg aaatgaaaca ataccggata cgcttcattg
      361 aagaagaaag ggcgcaatga ggtcagcctt cgcaacaaag cggcggcaga aaacctgccg
      421 ccgtgttgcc cgttaacgtt taacttcgta ggctaacgtt gccacaagca ctgtgtttcc
      481 ctgctggagc cggagtccgc aaagcgaatc cctcaccatc cagacaaaac acagaatcgt
      541 cacacagatg acgattaaca tcattaaaaa cgttcgctgt ggcatcatag cccctccatt
      601 gccttacggc ggttatcggg ctactataat gttgtctagg cattacagta gagcccttgg
      661 ttgattgata aaaaaatctc caggggcttt ctgctgtata ccgtccacaa aacgctttcg
      721 gcatacagcc tcaagcgccc gcagctattc taacgacttt cgtatctgca acaatccttt
      781 tatcacacgg ctaattcctc tggttagctc ttatcgcctg caaataaatt tttaaccttt
      841 ccgtttgatt taaattccag aaatggagac tttgtcatgt cttacaatcg acagcctgtc
      901 gcagaagatc ctatgcagat atggggcgct gttggtgtcc ttcttatcct gctgctattc
      961 gttatatggt tattcttgcc ggaagtggtt tacgccagct gcctgattct tcatacctta
     1021 tggggactgg ttgactgggg accatttcat aactatgcag caccacgata caacctgctt
     1081 gccatgaccg gtaacaacgc cgccaacata agctatagcc agtgggttaa cgtcatggag
     1141 cagaccatag gtattctgtg gatgtacctc ttacctgtga cactctggtg tttgtgggag
     1201 tggtatcaac atccgggaca aagtcgtttc acccgcagac cagtagatat tacccgcctg
     1261 ccatacatct ttgcttcgtt gtcccctgct atcgcccctg tactggcaga tggtgatccg
     1321 gaaaaactct ttcatggtgg caaacgacct gaacggcggg ttgctctcac cccggaagca
     1381 tttgtcgagc agcatactct catcaccaat atgcagctcg atgttgcagc tgcaagacga
     1441 tgtttcatgg cccagttggg taaaccttta acgtcctgga aagacatggc accacatgaa
     1501 aaagcgctct ttgccatatt tggtcttcag tatttcctgg atgatcgtaa ggcagcatta
     1561 aagctcatgg atacactgaa cctctcatgc agaattaaaa gcaaacggga cagtgggaaa
     1621 ttctgtaccc ctgtttattc actggccaaa tcagctttcc agagagtgat taaaagcaac
     1681 ggagcacagc aatggctgaa acaacatcgc tacgtccgca gtgggctggt ctggctgtat
     1741 gcacatgacc tgcggcttac gccacccaac tggatttggc tgaaaggcgt ggacaggacg
     1801 ctgttttatg cattacaccg cgccaatacc acgaaaggat ttattgaagg cgctggcgtg
     1861 gtggccgttg cacgtgcaga ggccgaagcc atgcgttttg gccttccctg ccctgaaccc
     1921 tgtgttgatg aagcggtgga aggcttacgg cgggatatgc tcagtctcgg tcttatctgg
     1981 gatgaaccac aaccagatcg cgaccggaaa cgccgcatcc tgaccaactg gtccctcacc
     2041 gacgacatcc tgccccgcac acctgctaca gacaacgaat tctgattatc cggagaactg
     2101 aaaatgacaa tcgagtactt tgcaacacgt attgcaaaac atatttccgt gcaggcaatc
     2161 tggccggatg gcaggacaga aattattgcc gtccttccac gggatgccct gtcagggctg
     2221 gtaacacgaa ataatcaact gattcatata gcctttgacg ccatgggcac ccgtaacaga
     2281 gaaaccgtcc tgattgataa acaggagcat aacgctgacc agatacttac agctattgat
     2341 tcctgcctgc gccttgaata tctgatggaa cgccgcttca gcagaacacc tttgttccgt
     2401 ggcataatcg ccagcgttgt gctgttcgta atggcaacga ttggcttctc tctgttccgc
     2461 tatgttgatc gtgtattctg ggatgataca acgcctgaag cagtacagac agcaggtgag
     2521 ccacgactgc ttccccctca cctgaatcac actgtgccat taaatgaagg cattcaactc
     2581 ccggttcccc cgaaaaaaga cgtccaggta ccggagaaaa caatcacaag caaaaatccg
     2641 gaagccgcag ctgcgcgaca taatctggct actgttctga agcggaacgc cgatcggggc
     2701 atgtttacca tcaacctgtc atccggtcat gaacgaaccc tgttcgcatt tctcgatcct
     2761 gcctgtccta actgcagact tctggagccg gccttaaaac gcctggccag cgacttcaat
     2821 gtcgttatct acccggtttc agttattggt ggagaggaat ccaccgaccg tgtggcacca
     2881 ctgctgtgtg aaaaagatgc tcagaaacgt gctgccggct ggcaccgact gtattccgca
     2941 gataacggaa tgatgacgcc atcagaagaa acaacgcctg cagatgaaac atgcctgaaa
     3001 gcagccagag ctgcgattga tgttaacaat gtcgccttcc gtaagttcgg atttgccggt
     3061 accccatggg tgctgtccga caccggctgg catctgccta caggaattct gcaggaaact
     3121 ggcacgctta atttattcct gaaaaccacc gacagcgagt ctggccatga gtgaacatcg
     3181 cgttaacccc gagcttttac atcgtacagc ctggggaaat cctgtctgga atgcactcca
     3241 gagtctcaat atctatggct tttgcctggt ggcaagtctt gtggccagtt ttatctggcc
     3301 acttgcatta ccggcatgtc tgttgttcac actgataaca atgctggtat ttagtctgca
     3361 gcgatggcgc tgccctcttc gtatgcccat gacgctggaa tgtgctgacc cgtcacagga
     3421 cagaatgatt aaaagaagtc ttttcagctt ctggccaacc ctgttccagt atgaggtcat
     3481 tctggaatca ccggccagcg gcatattcta tgttggttat caacgcgtca gggatattgg
     3541 cagggagtta tggctgagta tggacgatct gacccgccat ataatgtttt tcgccaccac
     3601 cggtggcggt aaaacggaaa ccatctttgc ctgggcgatt aacccacttt gctgggcaag
     3661 aggattcacc cttgtcgatg gcaaggcaca gaatgatacg gcaagaacga tctggtatct
     3721 ggccagacgc tttggacgtg aagacgatgt ggaagtcatt aacttcatga acggaggaaa
     3781 atcccgcagt gaaatcattc tgagtggtga aaaaacgcgt cctcagtcaa atacctggaa
     3841 tcccttctgt tacagcaccg aggcattcac agcggagaca atgcagtcaa tgctgcccca
     3901 gaacgtccag gggggcgagt ggcagtcccg ggccattgcc atgaataagg cactggtatt
     3961 tggtactaaa ttctggtgtg tgcgcgaagg caaaacaatg tcgctgcaga tgctccgtga
     4021 acatatgact ctcgaaggga tggccaaact ttactgtcgt ggcctcgatg atcagtggcc
     4081 ggaagaagct atcgcaccgt tacgtaacta ccttcaggac gttcccggtt tcgacctgtc
     4141 gctggtcagg acaccatcag cctggacgga agaacccaga aaacaacatg cttatctctc
     4201 cgggcagttt tctgaaactt tcagcacatt cacggaagca ttcggggata tttttgctga
     4261 agattccggt gatatcgata tccgtgacag cattcacagc gacaggattc tgatggtaat
     4321 gatccccgct ctggatacat cagcgcatac aacgtctgct cttggacgaa tgtttatcac
     4381 ccagaaaagt atgattctcg cccgtgattt gggctaccgc ctcgaaggaa ctgattccga
     4441 tgcactggaa gtcaaaaaat ataaggggcg tttcccttat ctgtgt
//
LOCUS       DAJOSY010000194         4386 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJOSY010000194 DAJOSY010000000
VERSION     DAJOSY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.194"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..807
                     /gene="mppA"
                     /locus_tag="OHN27_004252"
     CDS             <1..807
                     /gene="mppA"
                     /locus_tag="OHN27_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCQ2288586.1"
                     /translation="KNMYQKLLKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLAL
                     SMTIDRRLMTEKVLGTGEKPAWHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAA
                     GYGPQKPLKLTLLYNTSENHQKIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNF
                     DVIRASWVGDYNEPSTFLTLLTSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYN
                     AAEKILMEQAPIAPIYQYTNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(858..1889)
                     /gene="ynaI"
                     /locus_tag="OHN27_004253"
     CDS             complement(858..1889)
                     /gene="ynaI"
                     /locus_tag="OHN27_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCQ2288587.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            2133..2390
                     /locus_tag="OHN27_004254"
     CDS             2133..2390
                     /locus_tag="OHN27_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCQ2288588.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(2440..3390)
                     /gene="uspE"
                     /locus_tag="OHN27_004255"
     CDS             complement(2440..3390)
                     /gene="uspE"
                     /locus_tag="OHN27_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCQ2288589.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(3542..4294)
                     /gene="fnr"
                     /locus_tag="OHN27_004256"
     CDS             complement(3542..4294)
                     /gene="fnr"
                     /locus_tag="OHN27_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCQ2288590.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT         1209 a         1000 c         1027 g         1150 t
ORIGIN      
        1 aaaaatatgt atcagaagct gttgaaggat attccggggc aggtttatac gccgccacag
       61 ctcgggacct attattatgc gtttaacacg caaaaagggc cgacggcaga tcagcgcgtt
      121 cgcctggcat taagtatgac gatagatcgc cgcctgatga ccgaaaaagt gttgggaacg
      181 ggcgaaaagc cagcgtggca ttttacacca gatgttaccg ccggatttac gccggaacct
      241 tcgccgtttg aacaaatgag tcaggaagaa ctgaatgcgc aggcaaaaac tttgttgagt
      301 gcagctggtt atggtccgca aaaaccgctg aagctgacgc ttttgtataa cacttcagaa
      361 aaccatcaaa aaattgcgat tgctgtagca tcgatgtgga aaaagaacct tggcgtagat
      421 gttaaattgc aaaatcagga atggaaaacc tatatcgata gccgtaacac cggcaatttt
      481 gatgttatcc gcgcctcgtg ggtgggggat tataatgaac cctccacttt cctgacatta
      541 ttaacgtcaa cgcattcagg aaatatttca cgctttaaca atccggcata tgacaaagtt
      601 ctggcccagg catcgacgga aaataccgtt aaagcgcgta atgccgatta caacgcggca
      661 gaaaaaatcc tcatggagca agcaccgatt gcaccaattt atcaatatac caatggacga
      721 ttaatcaagc cgtggctgaa aggttatccc attaataatc ctgaagatgt ggcgtacagt
      781 cggactatgt atattgtgaa gcattgatgt gatgggaact ggcgttaccc ttgtgcataa
      841 cgccagtgat gtctgattta gcgaccctgt tccggcggtg taatgttatc catatacagc
      901 gtctggctgg ggaaggcaaa gtccgcgccg tgtgactgta caatatcgat aatcttcaaa
      961 taaacgtctt gctgtgcagc aagccattca gcccataccg tggttttggt aaagcaataa
     1021 accataatat tcaatgaaga gtcagcaaac tggttgaaat aaaccagtaa ggtttgtcgc
     1081 tggtcgatgg ccgggtgatt tttcagcatc tcacgtacag cttcgacaat aacgcccact
     1141 tttgccgcat cctcataacg taaaccaatg gtcgtggtaa tgcggcggtt ggtcattcgt
     1201 cctgggtttt ctacgctgat cgacgaaaac agcgagttcg gtacgtacaa tggacgatta
     1261 tcaaaggtcg taattttggt aattcgccag ccaatttccg ctactgtacc ttcgatattt
     1321 ctgtccggtg aacggatcca gtcgccaata ctgaaaggac ggtcgaaata gagcataatc
     1381 ccggaaaaga agttactcag aatatcttta ccggccatac cgacagccag accaccaata
     1441 ccaccaaagg tcagcaagcc agaaaggctc atgccgaaat gttcgccata aagcagaaca
     1501 agcaccacaa taatggtgat tttgatgata cgcgacataa tccgcgcact ggtgatatcg
     1561 cgaccttttt taatctgctg tttttcaaac tgattaatca gcagaaatag cttaatcgtc
     1621 agaataaccg caatcaggga cgtacagata aaatcgataa cgcctggggt gataaatttg
     1681 agtttatagt tttctataac ataattaata atgctaccaa cagcactgat aattatggtg
     1741 tagattaaaa attgcaccgc atggaataaa aatccttttc ttttacgatt tccacggcga
     1801 aaccaaaagc tcatcagaat caatgctgcg cagctaccga aaataatgac cagattaagc
     1861 gcattatttg taaacagttc agcgatcatt gttttatcag gctcctccag ataattgtcg
     1921 tcatgccgga aacccctggc ggggctattt taccgcgaca attcattcag atcatcaata
     1981 gtcagggaag gaagtagcaa cattagctaa ctaatcaatg cgttgattgt aaatccagct
     2041 aagaggtgag gttttcagag cagacaacgg tgaaatgtca tggtattgtt acgtttaggt
     2101 aacaagaaat ttgtctgcac aaggattaca tcatgattat ggcgaaactg aagtcagcga
     2161 aagggaagaa atttctcttt ggtttgttgg cggttttcat tattgcggcg tcggttgtga
     2221 ctcgcgcgac catcggcggc gttatagaac agtacaatat tccgctgtct gagtggacga
     2281 catcaatgta tgtgattcag tcatcgatga tttttgttta tagcctggtc tttactgtgt
     2341 tgctggcaat cccgttggga atttatttcc ttggcggcga agagcagtaa gtaaaaaata
     2401 ggcccgataa ctcgggcctt gtcagttatt gaagagtcgt taatcgtctt cttcgtcatc
     2461 cagttcaacg ggtgtctgat actggtcagg tttaatgacc agcaggtcgc agcgaagatg
     2521 atcaatcacc tgttccgccg tgttgccgag gaatgctgct gaaataccgg tgcgtccgac
     2581 cgtgcccaga accacaattc ccgcctgtaa atgctccgcc aaatcaggaa tcacctcttc
     2641 aggcagacct ttttctacgt gcgtcatgtt ttcattaatg ccgaatttct gccgcagagc
     2701 tttcattgcc agcaaatgtt gcccacgaat ggcatcgtta taaacgctcg ggtcaaattc
     2761 cggcagttca atcgcgatat taattggcgt taccggataa gcgccaacca gatgaacttc
     2821 ggtatggttg acttgttctg ccagttcgat cgtctctttg accagttttt cattgagcgc
     2881 attatgatac ggctcttcac tggcgagatt caccgccacc agcgccttgc ctccttccgg
     2941 ccacggctgg tctttcacca tccacaccgg gcttgggcat ttgcgtaaca gatgccagtc
     3001 cgttggcgta aaaatcaccg cttccagacg gtcatgttgg tgcgccattt ttagcaccaa
     3061 atcgtgtccg ccgctgatca cttcctgaat gatggcttcg aaaggacggt tatgccagac
     3121 cactttaatt tcaatgggaa cgccagcatt gagataatat tttgcctgct cgtggatcca
     3181 ggctgtacgc tggctgatga cgccctgacg catagcggta cgttcgtccg gggagagcag
     3241 ggtggtcatt tcgtatgaga agtcatagat cggcaaaaag gctttaattt tgccaccaat
     3301 ccgttgatgt aaataaacag ctcgccgcaa tgctggttgg tcgtcctggt taggatcgat
     3361 aacaacgagc atgttctgat acatagccat acagggtctc cttacaacaa ctgtcaacgc
     3421 agtttgtaat taaaagatta acccatatct ggtgaatgaa acagtgatga accttctgcc
     3481 agatcaataa atcagaaaaa tttaatgata tgacagaagg atagtgagtt atgcggaaaa
     3541 atcaggcaac gttacgcgta tgaccagcaa gctgggccag cgcatcgtta ttttcgatgg
     3601 tgatgtattt acctttgact gccagcatac cgcttttctg gaagcgaccc agcagacggc
     3661 tgatggtttc taccgtcagg cccagatagt taccgatatc gccacgagtc atcgtcaggc
     3721 ggaattcacg aggggagaag ccgcgttggg caaaacgacg ggacaggttg tagatgaatg
     3781 cagccagacg ttcctcggca tttttcttcg acaacagcag gatcatgtcc tgatcgcctt
     3841 tgatttcacc gctcatcaga cgcatcatct gctgacgcag attcggcatt ttaccggaca
     3901 aatcgtccag cgtttcgaac gggatttcac ataccatcga ggtttccagc gcctgagcga
     3961 agctcgggtg atggccgctg ccgatagcat caaatcccac caggtcacct gctaaatgaa
     4021 aaccagtgat ttgctcgtcg ccttgctcag tgatggtata acttttaatc gtaccggagc
     4081 ggatggcata aagcgattta agttcatcac cagccttaaa cagcgtctgg cctttctgaa
     4141 taggcttctt ccgctcaatg atattatcaa gctgatcaag ctcatgttcg ttgagtgtga
     4201 acgggatgca aagctggctg atgctgcaat cctggcaatg gatagcacaa ccgccagact
     4261 gaatgcgccg tataattcgc ttttccggga tcataggtct gctcaagccg taattgatat
     4321 ttgtcaattt taacatcttt ttagggagca agtaagtcta agcaaacctt aacagcagag
     4381 aattcc
//
LOCUS       DAJOSY010000195         4306 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJOSY010000195 DAJOSY010000000
VERSION     DAJOSY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.195"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..221
                     /locus_tag="OHN27_004257"
     CDS             <1..221
                     /locus_tag="OHN27_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCQ2288591.1"
                     /translation="LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLL
                     EDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            377..1912
                     /gene="gadC"
                     /locus_tag="OHN27_004258"
     CDS             377..1912
                     /gene="gadC"
                     /locus_tag="OHN27_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCQ2288592.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2043..3362
                     /locus_tag="OHN27_004259"
     CDS             2043..3362
                     /locus_tag="OHN27_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCQ2288593.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3647..3688
                     /locus_tag="OHN27_004260"
     CDS             3647..3688
                     /locus_tag="OHN27_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288594.1"
                     /translation="MVQLVDLARCVSF"
     gene            3726..>4306
                     /gene="dosC"
                     /locus_tag="OHN27_004261"
     CDS             3726..>4306
                     /gene="dosC"
                     /locus_tag="OHN27_004261"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCQ2288595.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWE"
BASE COUNT         1081 a         1038 c         1044 g         1143 t
ORIGIN      
        1 acctctctga acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact ctcggcggtg
       61 aagccaccga catcgtggtg atgcgcatta tgtgtcgtcg cggcttcgaa atggactttg
      121 ctgaactgtt gctggaagac tacaaagcct ccctgaaata tctcagcgat cacccgaaac
      181 tgcagggtat tgcccaacag aacagcttta aacatacctg ataacgttta acggtaacgg
      241 tgtcccgaaa cgaacccgtt tcgggacaat ttccaaagtc tgttcactgg cattagcaac
      301 ggaaaatatt gttctgaata cgcttcagaa caaaacaggt gcggttccga caggaatacc
      361 gttttagggg gataatatgg ctacatcagt acagacaggt aaagctaagc agctcacatt
      421 acttggattc tttgccataa cggcatcgat ggtaatggct gtttatgaat accctacctt
      481 cgcaacatcg ggcttttcat tagtcttctt cctgctatta ggcgggattt tatggtttat
      541 tcccgtggga ctttgtgctg cggaaatggc caccgtcgac ggctgggaag aaggtggtgt
      601 cttcgcctgg gtatcaaata ctctggggcc gagatgggga tttgcagcga tctcatttgg
      661 ctatctgcaa atcgccattg gttttattcc gatgctctat ttcgtgttag gggcactctc
      721 ctacatcctg aaatggccag cgctgaatga agaccccatt accaaaacta ttgcagcact
      781 catcattctt tgggcgctgg cattaacgca gtttggtggc acgaaataca cggcgcgaat
      841 tgctaaagtt ggcttcttcg ccggtatcct gttacctgca tttattttga tcgcattagc
      901 ggctatttat ctgcactccg gtgcccccgt tgctatcgaa atggattcga agaccttctt
      961 ccctgacttc tctaaagtgg gcaccctggt agtatttgtt gccttcattt tgagttatat
     1021 gggcgtagaa gcatccgcaa cccacgtcaa tgaaatgagc aacccagggc gcgactatcc
     1081 gttggctatg ttactgctga tggtggcggc aatctgctta agctctgttg gtggtttgtc
     1141 tattgcgatg gtcattccgg gtaatgaaat caacctctcc gcaggggtaa tgcaaacctt
     1201 taccgttctg atgtcccatg tggcaccaga aattgagtgg acggttcgcg tgatctccgc
     1261 actgctgttg ctgggtgttc tggcggaaat cgcctcctgg attgttggtc cttctcgcgg
     1321 gatgtatgtc acagcgcaga aaaacctgct gccagcggca ttcgctaaaa tgaacaaaaa
     1381 tggcgtaccg gtaacgctgg tcatttcgca gctggtgatt acgtctatcg cgttgatcat
     1441 cctcaccaat accggtggcg gtaacaacat gtccttcctg atcgcactgg cgctgacggt
     1501 ggtgatttat ctgtgtgctt atttcatgct gtttattggc tacattgtgt tggttcttaa
     1561 acatcctgac ttaaaacgca catttaatat ccctggtggt aaaggggtga aactggtcgt
     1621 ggcaattgtc ggtctgctga cttcaattat ggcgtttatt gtttccttcc tgccgccgga
     1681 taacatccag ggtgattcta ccgatatgta tgttgaatta ctggttgtta gtttcctggt
     1741 ggtacttgcc ctgcccttta ttctctatgc tgttcatgat cgtaaaggca aagcaaatac
     1801 cggcgtcact ctggagccaa tcaacagtca gaacgcacca aaaggtcact tcttcctgca
     1861 cccgcgtgca cgttcaccac actatattgt gatgaatgac aagaaacact aaccatgtct
     1921 gaatggttat aagacaaggg agcgataatt catcgctccc tttttcgtgc ttgctgtcgt
     1981 attgacttaa cggggataag tacgagaatg agcgcacatc tgtttaccgg aaaccagcac
     2041 atatggatat ctgctcccga aacgagaaat tagcgattag aagaccggcg atactggttg
     2101 cactggcact tttactgtgt agttgtaaaa gcacgcctcc agagtccatg gtgacaccac
     2161 cagcgggttc aaagccacca gccacgacgc aacaatcgtc acaaccgatg cgtggcatct
     2221 ggctggccac ggtgtctcgc ctcgactggc caccggtttc ctcggttaac attagtaacc
     2281 ccaccagccg ggcccgtgta caacaacagg cgatgatcga caaactggat catctgcaac
     2341 gtctcggtat aaacacggtc tttttccagg tcaagccgga cggtaccgcc ctgtggccat
     2401 cgaaaatttt gccgtggtcc gatcttatga ccggtaagat tggtgaaaat ccgggttacg
     2461 atccgctgca atttatgctc gacgaagccc acaagcgtgg gatgaaagta cacgcctggt
     2521 ttaaccccta tcgcgtatcg gttaatacga agcccggtac tatcagggaa ctgaatagca
     2581 ctctgtctca acaaccggcg agcgtctatg tgcaacaccg cgactggatc agaacgtctg
     2641 gcgatcgctt tgtcctcgac ccgggcatcc ctgaggttca ggactggatc acatcaatag
     2701 tcgcagaagt ggtttcccgc tatcccgtag atggcgtgca gtttgacgac tatttctata
     2761 cggagtcacc gggttcacgg ctaaatgata acgaaacgta ccgtaaatac ggaggcgcat
     2821 ttgcgtcaaa agcagactgg cggcgcaaca atactcagca gttaattgca aaggtatcgc
     2881 acaccattaa aagcattaag ccgggagtcg aatttggtgt tagcccggca ggcgtgtggc
     2941 gtaaccgatc acacgatccg ctcggttccg atacccgagg cgcggcagcc tatgacgaat
     3001 cctacgctga cacccgtcga tgggtggaac aaggattgct ggattacatt gctccccaaa
     3061 tttactggcc gttctcacgg agtgccgcgc gttatgacgt gttggcaaaa tggtgggcgg
     3121 atgtcgttaa accgaccagg acccgcctgt atatcggtat cgccttctat aaagtgggtg
     3181 aaccttcaaa gatagagcca gactggatga ttaacggcgg cgtaccggaa ctgaaaaagc
     3241 agctcgatct taacgatgct gtgcccgaaa ttagcggcac catcttgttc cgtgaggact
     3301 atctgaataa accgcagact caacaagcgg tcagctatct gcaaagtcgc tggggcagtt
     3361 aaactcccat tcttactttg taacaagccg gtgctttacc caccggcttg ttaccctctt
     3421 aaactatccc actaataatc atgcttactt aagtcaaatt aaccacactt agttaaacag
     3481 ccataaatcc ctaataaaaa tgtatgaata tgaaaaacca tacgatattc gctgtcaccg
     3541 aaaaataatt acgatgggct atactcttct gaagaacagt agcgtagatt gaaactgtcc
     3601 taaatcgctt atttcttttc agtatatctt catatttcag gagaatatgg tgcagttagt
     3661 cgatctcgca cgttgcgttt cattttgatt ccgtctgatt tcccagcctt ataagggtgt
     3721 gaattatgga gatgtatttt aaaagaatga aagatgagtg gaccggactt gtcgaacagg
     3781 cagatccgcc cattcgtgct aaagccgcgg aaattgccgt tgcgcatgct cattatctga
     3841 gtatcgagtt ttatcgaatt gtccgcatcg acccgcatgc cgaagaattc ttgagtaatg
     3901 aacaagttga gcggcagttg aagagtgcga tggaacgctg gattattaac gtgctttctg
     3961 cccaggttga cgatgtcgaa aggctaatac aaatccagca taccgtcgcg gaagtgcatg
     4021 cccgcatagg aattccggta gaaattgtcg agatggggtt tcgggtgctg aaaaaaatcc
     4081 tctatccggt gatcttctct tcggattatt ccgccgcaga aaaacttcag gtctaccatt
     4141 tctcgattaa cagtattgat atcgcgatgg aagtgatgac tcgcgcgttt acctttagtg
     4201 acagtagtgc ctcaaaggaa gatgaaaact atcgtatctt ctcgttactg gaaaacgccg
     4261 aagaagaaaa agaacggcaa atagcctcaa tactttcatg ggaaat
//
LOCUS       DAJOSY010000196         4297 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJOSY010000196 DAJOSY010000000
VERSION     DAJOSY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.196"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OHN27_004262"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OHN27_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2288596.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(249..324)
                     /locus_tag="OHN27_004263"
     tRNA            complement(249..324)
                     /locus_tag="OHN27_004263"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Thr,seq:ggt)
     gene            complement(331..405)
                     /locus_tag="OHN27_004264"
     tRNA            complement(331..405)
                     /locus_tag="OHN27_004264"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Gly,seq:tcc)
     gene            complement(522..606)
                     /locus_tag="OHN27_004265"
     tRNA            complement(522..606)
                     /locus_tag="OHN27_004265"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Tyr,seq:gta)
     gene            complement(615..690)
                     /locus_tag="OHN27_004266"
     tRNA            complement(615..690)
                     /locus_tag="OHN27_004266"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Thr,seq:tgt)
     gene            1052..2002
                     /gene="coaA"
                     /locus_tag="OHN27_004267"
                     /gene_synonym="panK"
     CDS             1052..2002
                     /gene="coaA"
                     /locus_tag="OHN27_004267"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCQ2288597.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2031..2996)
                     /gene="birA"
                     /locus_tag="OHN27_004268"
     CDS             complement(2031..2996)
                     /gene="birA"
                     /locus_tag="OHN27_004268"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCQ2288598.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2993..4021)
                     /gene="murB"
                     /locus_tag="OHN27_004269"
     CDS             complement(2993..4021)
                     /gene="murB"
                     /locus_tag="OHN27_004269"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCQ2288599.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1107 a         1084 c          958 g         1148 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtagtga ttgcagcggt cagcgttgtt
       61 ttaccatggt caacgtggcc gatagtaccg acgttaacgt gcggttttgt acgttcaaac
      121 ttttctttag acatcgattg tccctctaag acacggataa atcggtgata tcaccacatc
      181 aaccaggcaa catgcccgac ttgttgaatg caataaacag aaggaaaaaa cagggaggag
      241 aaaagaagtg gtgctgatag gcagattcga actgccgacc tcacccttac caagggtgcg
      301 ctctaccaac tgagctatat cagcacatct tggagcgggc agcgggaatc gaacccgcat
      361 catcagcttg gaaggctgag gtaatagcca ttatacgatg cccgcatcct ggaactcggc
      421 tacctgattt tcattctgca ctgaatatcg agagaagctc tctttattcg agccggtaag
      481 cgaacttatc gtctcgggct acgccatcgc gtggccgaaa ttggtggtgg gggaaggatt
      541 cgaaccttcg aagtctgtga cggcagattt acagtctgct ccctttggcc gctcgggaac
      601 cccaccggac ttgatggtgc cgactaccgg aatcgaactg gtgacctact gattacaagt
      661 cagttgctct acctactgag ctaagtcggc atcaagtagc gcgcattcta tggagacatg
      721 cgagttcatg caactaaaaa attgcataat ttgttttatt ggtcacattt tatgcgacac
      781 gatgaagaaa cagccgaacg gagggcagat tagcacactt tttcaacatc attgtgctca
      841 acaatgcgct cctgctaaac cataattctt tttatcagat ggaatatctg tcacattgct
      901 tttcaacgat agcttcctgg cagagatttt ttcttattat tcctccccat ctggtgttac
      961 cctcctgccc attaacccat tcaacagaac tgtgacgcgc catggcaaat atcgctttgc
     1021 cgatagagct atgaccgcca gaaacatgct tatgagtata aaagagcaaa cgttaatgac
     1081 gccttaccta cagtttgacc gcaaccagtg ggcagctctg cgtgattccg tacctatgac
     1141 gttatcggaa gatgagatcg cccgtctcaa aggtattaat gaagatctct cgttagaaga
     1201 agttgccgag atctatttac ctttgtcacg tttgctgaac ttctatataa gctcgaatct
     1261 gcgccgtcag gcagttctgg aacagtttct tggtaccaac gggcaacgca ttccttacat
     1321 tatcagtatt gctggcagtg tcgcggtggg gaaaagtaca accgcccgtg tattgcaggc
     1381 gctattaagc cgttggccgg aacatcgtcg tgttgaactg atcactacag atggcttcct
     1441 tcaccctaat caggttctga aagaacgtgg tctgatgaag aagaaaggct tcccggaatc
     1501 gtatgatatg catcgcctgg tgaagtttgt ttccgatctc aaatccggcg tgccaaacgt
     1561 tacagcccct gtttactcgc atcttattta tgatgtgatc ccggatggag ataaaacggt
     1621 tgttcagcct gatattttaa ttcttgaagg gttaaatgtc ttacagagcg ggatggatta
     1681 tccacacgat ccacatcatg tatttgtttc tgattttgtc gatttttcga tatatgttga
     1741 tgcaccggaa gacttacttc agacgtggta tatcaaccgt tttctgaaat tccgcgaagg
     1801 ggcttttacc gacccggatt cctattttca taactacgcg aaattaacta aagaagaagc
     1861 gattaagact gccatgacat tgtggaaaga gatcaactgg ctgaacttaa agcaaaatat
     1921 tctacctact cgtgagcgcg ccagtttaat cctgacgaaa agtgctaatc atgcggtaga
     1981 agaggtcaga ctacgcaaat aatttgcagg ggagcgaata ctcccctttc ttatttttct
     2041 gcactacgca gggatatttc accgcccatc cagggtttta ttattccatc ctgctcaagt
     2101 aataaagccc cctgtttgtc tattccgcgt gaaatgccaa atatttcttt atcaccaatg
     2161 ataagtttca ctgggcgatt aataaaatta tccagctttt cccagcgcga cagataaggt
     2221 gccaatcctt cttgttcgaa gagttccaac gcagcacgta attcacgtat tagcatggcc
     2281 gccaacgtat tacgatcgag attgatcccc gcttcctgca gcgtgatcca cccctgatta
     2341 acgacactct cttcaacacg gcgcattgcc atgttgatcc cggctccaat gactatttgc
     2401 gccgcatcgc cagttttgcc agtcagctcc acaagaatgc ctgccagctt gcgatcctgc
     2461 agatagaggt cattaggcca tttgacacga actttatctg ctcccagctt gcgtaatact
     2521 tccgccatca cgataccgat aaccagactt aaaccaatcg ccgccgccgg gccttgttcc
     2581 agacgccaga acatcgacaa atataagttt gcgccaaaag gcgaaaacca tttccgcccc
     2641 cggcgaccac ggccagcctg ctggtattct gcaacacagg catcgcccga tttaagctct
     2701 ccgatacgat caagaaggta ctgattcgtg gagtcaataa ctggcagcac ggctacacta
     2761 ccgccatcca gctgacccaa tatctgttca gcattaagta actggatggg ctcaggcagg
     2821 ctgtatcctt tacccggaac ggtaaagaca tcaacgcccc agtcacgcag tgtctgaatg
     2881 tgtttattaa tagccgcccg gctcattccc agcgtttcac ccaactgctc gccagagtga
     2941 aattcaccgt tcgctaacag ggcaatcaat ttcagtggca cggtgttatc cttcatgaaa
     3001 ttgtctccac tgcgctcact tcacctgatg caccaataaa gcggacttca ggctcaagcc
     3061 agacattaaa tttttcaccc actttttgtc ttacatggtg tgccagttgc accacatctt
     3121 cgctttttgc attgtcttca ttaatgagaa ctaacgcctg ttgacggtgc accgcagccc
     3181 cacccatttg catccctttt agctggcact gatcaataag ccaacctgct gccagtttta
     3241 ctgaaccacc cgcctggggg taatttggtg ctgttggaaa ttgcgccagt aatgctttag
     3301 ccgtttcggc agatacaaca gggtttttga agaaactacc ggcattgcca ttcacttttg
     3361 gatcagggag tttggtggtg cgcatatgac acaccgcatt aaatacttgc tgtggcgtta
     3421 ctgttgtagg atccagacga gttaagtcac catacgttag tacaggttgc cactcttttg
     3481 gcagacgcag acctacggct acaatggcga agcgatcctg gtattcatgt ttaaaaatac
     3541 tgtcgcgata gccaaaacgg cactctttgg cagttaagcg cacttgcttg cctgtcgcca
     3601 gttcaacaca atcaacataa gcgcaaactc gctgtaattc tacgccataa gcaccaatat
     3661 tctggatagg tgatgagccg acacaaccag gaattaatgc cagattttcc agaccaggca
     3721 taccttcctg caaagtgtat tttaccaggc gatgccagtt ttctccggct cctacatgta
     3781 aataccacgc atcaggttca tcatgaattt cgataccttt gatccggttg atgatcaccg
     3841 tgccgcgata gtcttccaga aaaagtacat tacttccttc acccagaata agaacgggtt
     3901 gtccttctgc ggttgcatac tgccaggcat tgagtaattg ttgttcgtct tcggcacata
     3961 caatgtgctg agcattatga tcaatgccaa atgtgttcca gggttttaag gagtggttca
     4021 tagctgcttt cctgatgcaa aaacgaggct agtttaccgt atctgtgggg ggatggcttg
     4081 tagatatgac gacaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     4141 tctgcttaat ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     4201 gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     4261 ccgacaaaca acagataaaa caaaaggccc agtcttt
//
LOCUS       DAJOSY010000197         4236 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJOSY010000197 DAJOSY010000000
VERSION     DAJOSY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.197"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            649..1383
                     /gene="tssI"
                     /locus_tag="OHN27_004270"
     CDS             649..1383
                     /gene="tssI"
                     /locus_tag="OHN27_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCQ2288600.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFTRPVMNTTVSAR"
     gene            1344..3899
                     /locus_tag="OHN27_004271"
                     /pseudo
     CDS             1344..3899
                     /locus_tag="OHN27_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            3911..>4236
                     /locus_tag="OHN27_004272"
     CDS             3911..>4236
                     /locus_tag="OHN27_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCQ2288601.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQTGKYSDIYK
                     ITSSDFQTATSEKKFIDIMNEAKEKDLGTYKKSTLKTEKITRGLFSNDEITLIYFSEY
                     SKRIVQ"
BASE COUNT         1080 a         1028 c         1200 g          928 t
ORIGIN      
        1 cgtgagagct gcacattatt ggcggggccg gggaaaacat cattttggga acaatgaatc
       61 aggtactctt accactgaag caagatcatc cgggcacctg gtggtggtgc agtgctttgg
      121 aaatggtccc ctcgggggaa ttgtcattat tttctatgct atttgcttat gatattcatc
      181 aggtgaattt aataaattcc taatttgaaa tatccactat taagccagtg tttaacgacg
      241 ttaagatggc aatgtgcaga tcatgttatc agggtggggc ggtagcgtat attacttata
      301 caaatcatca ttcatttcaa tgattgttat tgaattattt attgttgtga ataaaaagac
      361 attatgaata ttggtttgac tttgcatatc aggtttttta ttactcgctg taatgtacga
      421 gcctgtcgtg aataacgtcg agaacccatc cgttgcgttt gtgattattt tgttgactaa
      481 acagacaccc gtttctctga agtaaaatcc cagactaaat catcacataa ccatgacatt
      541 tttctgatat tccccggtaa cgccagatgt cgactcgctt atctcatccg cccgacatgt
      601 tgtacatgca atatgtgcgg attattattc attttcacgg aggttgctat gtcaaccgga
      661 ttacgtttca cactggaagt ggacggcctg ccgccggatg cttttgcggt ggtttccttt
      721 catctgaacc agtcactctc ttcgctcttt tccctcgatc tctccctggt cagccagcag
      781 tttctctccc ttgaatttgc gcaggtgctg gacaaaatgg cctacctgac gatatggcag
      841 ggcgatgaag tacagcgccg ggtgaaaggc gtggtgacct ggtttgaact cggggagaac
      901 gacaaaaacc agatgctgta cagcatgaag gtgcacccgc cgctgtggcg tgccggtctg
      961 cgccagaact tccgtatctt ccagaacgag gacatcaaaa gcatcctcgg cacgatgttg
     1021 caggaaaacg gggtgaccga atggagtccg ctgttcagcg agccgcatcc ttcccgtgag
     1081 ttttgtgtcc agtacggtga gactgattac gatttcctgt gccggatggc ggcggaggaa
     1141 ggcatcttct tttatgagga gcatgcttac aaaagtaccg accagagcct ggtgctgtgc
     1201 gacacagtcc gccatctgcc cgaatctttt gaaatcccat ggaacccgaa cacccgtacc
     1261 gaggtgagca ccctctgcat cagccagttc cgctacagcg cacaaatccg cccttcttcc
     1321 gtggtgacca aagactacac ctttaccaga cccgttatga atacgaccgt ttcggccaga
     1381 tgacggcggt ccaccgcgag gaaggcatca gcctttaccg ccgctatgac aaccgtggcc
     1441 ggttaacctc ggtgaaagac gcacagggcc gtgaaacgcg gtatgaatac aacgccgcag
     1501 gcgacctgac tgccgttatc accccggacg gcaaccggag cgagacacag tacgatgcgt
     1561 ggggaaaggc ggtcagcacc acgcagggcg ggctgacgcg cagtatggag tacgatgctg
     1621 ccggacgtgt catcagcctg accaacgaga acggcagcca cagcgtcttc agttacgatg
     1681 cgctggaccg gctggtacag cagggcggct ttgacgggcg gacgcaacgt tatcattatg
     1741 acctgaccgg aaaactcaca cagagtgagg attagggact tgtcatcctc tggtactacg
     1801 atgaatcgga ccgtatcact caccgcacgg tgaacggcga accggcagag cagtggcagt
     1861 atgatggcca cggctggctg acagacatca gccacctgag cgaaggccac cgtgttgccg
     1921 tccactatgg ctatgacgat aaaggccgcc tgaccggcga atgccagacg gtggagaacc
     1981 cggagacggg ggaactgctg tggcagcatg agacgaaaca cgcatacaac gagcaggggc
     2041 tggcaaaccg cgtcacgccg gacagcctgc cgccggtgga gtggctgacg tatggcagcg
     2101 gttacctggc gggaatgaag ctgggcggga cgccgctggt cgagtatacg cgggacaggc
     2161 tgcaccgtga gacggtgcgc agcttcggca gcatggcagg cagtaatgcc gcatacgaac
     2221 tgaccagcac atacaccccc gcaggccagt tacagagcca gcacctgaac agcctggtat
     2281 atgaccgtga ctacgggtgg agtgacaacg gcgacctggt gcgcatcagc ggcccgcgac
     2341 agacgcggga atacggctac agcgccacgg gcaggctgga gagtatgcgc accctcgcac
     2401 cagacctgga catccgcatc ccgtatgcca cggacccggc gggcaaccgg ctgccggacc
     2461 cggagctgca cccggacagt acactcacag tgtggccgga taaccgcatc gcggaggatg
     2521 cgcactatgt ctaccgccac gatgaatacg gcaggctgac ggagaagacg gaccgcatcc
     2581 cggcgggtgt gatacggacg gacgacgagc ggacccacca ctaccactac gacagccagc
     2641 accgcctagt gttctacacg cggatacagc atggcgagcc actggtcgag agccgctacc
     2701 tctacgaccc gctgggacgg cgaatggcaa aacgggtctg gcggcgggag cgtgacctga
     2761 cggggtggat gtcgctgtcg cgtaaaccgg aggtgacgtg gtatggctgg gacggagaca
     2821 ggctgacgac ggtgcagact gacaccacac gtatccagac ggtatacgag ccgggaagct
     2881 tcacgccgct catccgggtc gagacagaga acggcgagcg ggaaaaagcg cagcggcgca
     2941 gcctggcaga gacgctccag caggaaggga gtgagaacgg ccacggcgtg gtgttcccgg
     3001 ctgaactggt gcggctgctg gacaggctgg aggaagaaat ccgggcagac cgcgtgagca
     3061 gtgaaagccg ggcgtggctt gcgcagtgcg ggctgacggt ggagcaactg gccagacagg
     3121 tggagccgga atacacaccg gcgcgaaaag ttcattttta ccactgcgac caccggggcc
     3181 tgccgctggc gctcatcagc gaagacggca atacggcgtg gcgcggggag tatgatgaat
     3241 ggggcaacca gcttaatgaa gagaacccgt attacctgca ccagccatac cgtctgccgg
     3301 ggcagcagca tgatgaggaa tcagggctgt actataaccg gaaccggtac tatgacccgc
     3361 tacaggggag gtatattaca caagacccca ttgggctggc ggggggatgg aacctgtata
     3421 attacccact gaatccgata ataaggatgg atcctttggg tttgtataat ttatatcaat
     3481 tattatatga tgtttggcat gatgattcat atggaacatc atcaattgat attactggca
     3541 gtggagatct aatatcatta ggtggtcatg caggacttgg cgttgcgttt gctaaaaaga
     3601 aaggtgaaat gttatctgat atttgtattt atgctacagc atgcggacat gcaggaattg
     3661 gtggtgggat aaatgcggct atcacatatt ctgagaccaa gtctttacct acatcgggag
     3721 tcagcaattc agtaggtgta acggttggcg gcggagttgg ggggcatttt gcgtatactt
     3781 atgtagtgga tgttgataat ccagaatcat cgacagaatc tgttggtatc ggtgcaggtg
     3841 ttgacgcttc agttatgact ctggcttgta gaacgtggca agaatgctgg gtcaattaac
     3901 aggaggaaat atgaaaaaat atatattttt agttttattt ttattgtgtg gttgttctcc
     3961 agtggacata gagccagcta gacaagaagt taatatcttt cgtggtttat atcagacagg
     4021 gaaatacagt gatatatata aaatcacttc aagtgatttt cagactgcca catctgaaaa
     4081 aaaatttatc gacataatga acgaagcaaa agaaaaagat cttgggactt ataaaaaatc
     4141 aacccttaaa actgaaaaaa tcactcgcgg attattttca aacgatgaaa ttacattgat
     4201 atatttctca gaatattcaa aaagaattgt ccaaga
//
LOCUS       DAJOSY010000198         4211 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJOSY010000198 DAJOSY010000000
VERSION     DAJOSY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.198"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(1..133)
                     /locus_tag="OHN27_004273"
                     /pseudo
     CDS             complement(1..133)
                     /locus_tag="OHN27_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="response regulator in two-component regulatory
                     system with KdpD; regulates the kdp operon involved in
                     potassium transport; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            complement(209..1528)
                     /gene="potE"
                     /locus_tag="OHN27_004274"
     CDS             complement(209..1528)
                     /gene="potE"
                     /locus_tag="OHN27_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCQ2288602.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(1525..3723)
                     /gene="speF"
                     /locus_tag="OHN27_004275"
     CDS             complement(1525..3723)
                     /gene="speF"
                     /locus_tag="OHN27_004275"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCQ2288603.1"
                     /translation="MSELKIAVSRSCPDCFSTHRTCVNIDESNYIDFAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERIPAEYLSRISGVFEPSEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            complement(4034..4138)
                     /locus_tag="OHN27_004276"
     CDS             complement(4034..4138)
                     /locus_tag="OHN27_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCQ2288604.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
BASE COUNT         1146 a         1057 c          981 g         1027 t
ORIGIN      
        1 acccaatacc ggtttcagta ataaaatggc gaggccgggc cggatcctgt tccagttttt
       61 gtcgcagatg ccccatataa ttacgcaaat agttgctgtg ttcgatcgcg tttagaccct
      121 acatctgccg gatgcggcat aaataacgaa ctatttgcca acaaacagaa agggcgatca
      181 ttcaatcgcc cttttttact ttgttttatt aaccgtgttt atttttcagt tcaaagcgtg
      241 gtgataccag accatacagt gtccaaccaa ggaaggtcac gatggaaccg tacagcatgg
      301 cttcttcccc ggatgagtac agcgcataga aactgtacat cgcgccaaca aaagcaacaa
      361 agtttgcaac tttcgctttt gaggggggca cattagcgac cttctgaata atgactaatg
      421 ccgccattga cagaatatac ggaatgatat tggtcaccac ggccaggtta accagcacgt
      481 tgaactgact gtttagcgac gggctaatcg tcatcagcgc caacccgctc tggataatca
      541 caatggtcaa cattccctgc accggtgcat ccactttggt tacacgggag aaaattttag
      601 ggaagtagcc ttcatcagat gaagatttaa acacctgggc aatggtgaac tgccagccaa
      661 gtagcgaacc gcagcaggac atcaccatca gcgccataat gactttaccc acttccggcg
      721 tgaacatctg cgcgaaggcc agaccaaatg gtgccgttga atttgccagc tccatatttg
      781 gcacaatccc ggcaatcacg ttggtggaga cgatataaat caccgccgca cctaacgtac
      841 cgccgagtac cgcgattggc acattacgtt ccgggttttc cactacatca gtatttgcac
      901 acgcagactc cagaccaaga aaagcccaca gcgtcatagc gatggaagaa cctaccgcac
      961 tgaagaacgg tgcatgatgc ggattccagg aatcaacgta cagcgtcggg ctaaaccaga
     1021 accagccaat aatgcacaga ccgacgaccg gaataatgac cccccacacg gtaatgctac
     1081 tgatttgccc ggtaatgcgc gcaccaccaa agttagccac ggtacaaatc cacagcaccc
     1141 cgatggtcgc aagaccaatc tgcactggcg acaaactagc gccgagcagt tcggtgccgt
     1201 aaccaaccgc cgaaatagca atcgcgacgt tagcaatcag cagtgagacg ccgtaggtat
     1261 agttcgccat aaagttaccg gatttaccga aagcatactc ggcataaccg cccataccgc
     1321 ctgatttacg gctgaacata ccgcattttg cgaatgccca tgccagtgcc attgagccta
     1381 cggctgtcac cagccaggag ataattgaga ttgtcccgac ttcggcaagc tttgtcggca
     1441 gcatgatgat accggagccc atcatgttga ccatcgtcag tatggttaac tgaacgacgc
     1501 ccattttgtt cgatttagcc tgactcataa tttttcccct ttcaacaggg tgctttgcgc
     1561 atcacgaggc ttgatgacat agcaccaaac ttgcttacga ccatcatgtt cttcgatata
     1621 gacaccctgc agctccggtg caaaacctgg cagcaggttg atcccttctt ccagagcgct
     1681 gaagtaacgc agaacagcac caccccagat ttcacccgga acaacacaca gcacacccgg
     1741 aggataagga agcgcacctt cggcagcgat acggccttct gcatccggca gacgaaccag
     1801 ttccacttca ccgcgtaaat aggcgtagtt ggcttcttgc ggattcatgc tgacgcgtgg
     1861 gaagtgctcc ttacggaaca tctctttttg cagttgtttc acgttgtggc gggcatacaa
     1921 atcatgcatt tcctgacaca actgacgcag ggtataaccg gcgtagcgct cttcatgctg
     1981 tttgtagatg gaaggtagca cttctgctaa tggcgcgtcg gactcaagca gtttttcgaa
     2041 gcgtaccagc agggcaacaa gttgctgaag tttggccata tcttctgccg gagtcagcag
     2101 gaagaggatg gagttaagat cgcatttttc cggcactacg ccattttcgc gtaggaagtt
     2161 agcaagaatc gtcgcgggta caccgaacgc ttcatattcg ccgttacgtg catcaatacc
     2221 tggggttgtc agcaacagtt tgcatggatc gacaaagtat tgattctctg catagccttc
     2281 aaaagaatgc cagtgttccc ctggtacaaa ctggaagaag cgcagatcaa ccgcaatttg
     2341 cgctgtttca tacgactgcc agggtttacc atccaccagt tcaggtacga acggacgaat
     2401 atgctgacag ttatcgagga tcagtttgcg ggcattaatg ccatttacca cacaatccat
     2461 ccacatatta cgaccgctga caccttcatg cattttggcg ttgatatcca gtgcggcaaa
     2521 cagcggatag aacgggctgg tggaggcgtg catcataaag gcgttgttca tgcgtttgtg
     2581 cggtacataa cgctgttgcc ctttgatgtg gctgtctttt ttatgaattt gtgaagtctg
     2641 agaaaaacca gcctgttgtt tatgcacaga ttgcgtaacc agaatacccg gatcgttctc
     2701 attaagatcc agcaacagcg gcgaacagtc cgccatcatc ggaataaact gttcatagcc
     2761 gacccatgct gagtcaaaca ggatgtagtc acacagatga ccaattttat ccaccacctg
     2821 gcgggcgtta taaatcgtac cgtcgtaggt gcctaactga atcacagcga ggcggaaagg
     2881 acgagcctct tttgcccgct gcggtgcgac ttccgcgatc agctcacgca ggtaactttc
     2941 ttcaaaacag tgcgcatcaa tgccaccgat aaagccatac gggttacgtg ccgtttccag
     3001 ataaaccggt gttgcaccag cctgtagcaa cgctccgtgg tggttagatt tgtggttatt
     3061 gcggtcaaac agcaccagat cacccggtgt tagcagggcg tttaaaacca ctttgttaga
     3121 agatgaagtg ccatttaaaa cgaagtaggt tttatcggca ttaaacactt ttgccgcatg
     3181 ttgctgtgca atgcatggcg cgccttcgtg aatcagcaga tcgcccatcg ctacgtcggc
     3241 gttgcacaag tcggcacgga acagcgcctc accaaagtat tccacaaact gattccccgc
     3301 cggatggcga cggaaaaatt cgccgccctg atgaccaggg caatcaaacg cgctgttacc
     3361 ttgattgaca taatcgacca gtgcgcggaa gaaaggtggg cgcagttgag tttcataatg
     3421 gctggcagcg gtttctaact ggcgaccata aaattcttta cgtgcttcgc taggttcgaa
     3481 gacaccagaa atacgtgaaa gatattctgc tggtatacgt tcttcatttt ccgttgcaat
     3541 aaaaacagga atgccatagc cagtagcgtc tatttcatcg agttttccac gttccacatc
     3601 attgaccgat aaaataatgg cggcaaagtc aatataatta ctttcgtcaa tattcacgca
     3661 tgtacgatga gtggaaaaac aatccgggca agaacgacta acggcaattt ttaattctga
     3721 cattttgttc atctctttat ttcaggtaat agcaggtcct caatttctca aaggagaaat
     3781 cgatcacgcc ggaaaaaaag caaagctcca ccgctgataa ataaatcagt agtttgcttt
     3841 ttgcagatgc aataacccag accagcccgg taactggcta tactcgtcat ccttcaagtt
     3901 gcatatgctg cgactcgaat tatttagagt ttaaaagggt tagcagtata tgagcaccga
     3961 ggaatacagg tattaacctg taatgcttaa agtcagggag acctgtacgc tggggtgccc
     4021 tttagtcaga agactagcgg gcattaaaga aatgaaagtc aaaactatta cgatgggcaa
     4081 acttcatgat atgggttgtc cgccttatat gaggcattaa gcggttatta ttttccattt
     4141 acctttacct cttgctgtgt cagttgaagg tagagttatt ctatccatga aatggctata
     4201 atctgtgaag a
//
LOCUS       DAJOSY010000199         4167 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJOSY010000199 DAJOSY010000000
VERSION     DAJOSY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.199"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(366..758)
                     /locus_tag="OHN27_004277"
     CDS             complement(366..758)
                     /locus_tag="OHN27_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCQ2288605.1"
                     /translation="MYEGEERKKLSLYLHPEDSADCLALAEIETVPRKKRGELYRQAL
                     ITGLIMHQLDERIPAVLTALFTRELNADEVISQIARITGWKPSSGDLKEVLKALGGLQ
                     STVSPEHSQDDGEQARLKAARVKMQNLI"
     gene            complement(762..1736)
                     /locus_tag="OHN27_004278"
     CDS             complement(762..1736)
                     /locus_tag="OHN27_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbA family protein"
                     /protein_id="HCQ2288606.1"
                     /translation="MRIFCDDGSTNVKLAWFEGKTLKSAVSVNSFRHNWKVEGLGSSR
                     TYNYLLDGRKYTYDPVSEDAISTTHIEYQYSDTNVLAVHHALLNSGIEPQEIDLTVTL
                     PISEFYTADCQKNTLNIERKIRNLMREVTLNKGGTFTIKSVEVMPESLPAVFTRLVAD
                     NVGQYEKSLVIDLGGTTLDVGVIVGQFEDVSAVHGNPDIGVSMVTKATLTALKMASSD
                     TSPMIADELIKNRNNLDFVGQVVNEVSKLNLVLDTIDTAINKLGELVVDDLLQYRNVN
                     RVYIVGGGAALIADAVRKAWNHLGEKVVLMDEPQTALVQAIARFKAEE"
     gene            complement(1975..2349)
                     /locus_tag="OHN27_004279"
     CDS             complement(1975..2349)
                     /locus_tag="OHN27_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288607.1"
                     /translation="MADASKLRTKRTRSGLGVPPGPDEVATSLNAPEIAPAVAATDVQ
                     RQQADSSPVHHEPVVTQTVTEVYTRRDGRSARKTNRTLAFATRVTPAFDQEIRDIAER
                     EGLKLVEVLEKAVEAYKEKQGY"
     gene            complement(2349..2981)
                     /locus_tag="OHN27_004280"
     CDS             complement(2349..2981)
                     /locus_tag="OHN27_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCQ2288608.1"
                     /translation="MAWIVGFISQKGGVGKSTKARALAREASACGIKTKLADLDLEQA
                     TSAEWHRRRLSAGLSPVASVEVFSTAKQAIESAGDFDLLILDGPARASKGTSEIAKVA
                     DLIVQPTGASLDDLVPAIKVFNALVKEGIPRSKLVFALSRVGTEAEELDARAYISEAG
                     YETLSGCLFEKPAYRKAMNSGLAVTETRYKGLNERADELIQALIDKIGEE"
     gene            complement(3565..>4167)
                     /locus_tag="OHN27_004281"
     CDS             complement(3565..>4167)
                     /locus_tag="OHN27_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2288609.1"
                     /translation="MLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQRTEK
                     AARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARIWEV
                     TDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKS
                     ISSTEVYTKVFALDVAARHRVQFAMPEADAVALIKQLERR"
BASE COUNT         1070 a         1094 c          912 g         1091 t
ORIGIN      
        1 aattgagcgt tttttagtca gaacaatgac atttgactgt gtgcggattc agttattaga
       61 atactgtata tatatacagc aaaagaaaag gaagatgaga acatgatccg cattgaaatt
      121 cttttcgacc gccagagcac aaaaaaactc aaatcaggca cacttcaggc gctacagaat
      181 gaaatcgaac aacgtctgaa accacactac ccggaaatct ggctgcatat gtgggaatcc
      241 ccgtccttca gagtgaggag ctgtcaacca gcactccatt gactcaaatg acgggaaacg
      301 cggaaatcta aaaattaagc cgcacacccc gagaaatttt gcaggtttca cggagcgaga
      361 cttttttaga tcaggttttg catcttcacc ctagccgctt tcagacgggc ctgttcaccg
      421 tcatcctgag agtgttctgg tgaaactgta ctctgaagcc ctcccaacgc cttcagtacc
      481 tcttttaaat caccggagga gggtttccag cctgttatcc tggcaatctg actaatgacc
      541 tcatccgcat tcaattctct ggtgaatagc gcggttaaca cggcgggaat tcgctcatca
      601 agctgatgca ttatcaggcc ggttatcaaa gcctgccgat ataactcccc ccgttttttc
      661 cggggaacag tttcgatttc agccagtgca agacaatcgg cagagtcctc cgggtgaagg
      721 tagagtgaaa gttttttcct ttcttcgcct tcgtacatcg attactcctc cgctttgaag
      781 cgtgcaatag cctggaccag cgctgtctga ggttcatcca taagaaccac cttttcgcca
      841 aggtggttcc atgccttacg aacagcatca gcaatgagtg cagccccgcc accgacaata
      901 tatacgcggt ttacgtttcg atactgcagc aaatcatcaa caactagctc gcccagttta
      961 tttatcgctg tatcaatggt atccagcacc agatttagtt tagagacctc atttacaacc
     1021 tgccccacaa aatccaggtt attcctgttt ttgatcaact cgtcagcaat cattggactg
     1081 gtatcactgc tggccatttt cagtgcagtg agcgtggctt tcgtgaccat tgatacacca
     1141 atatcaggat taccatgtac agccgaaaca tcctcaaact ggccaacaat aaccccgaca
     1201 tccagagttg taccaccgag atcgataacc aatgattttt cgtactgacc aacgttgtca
     1261 gcaactaggc gagtaaatac agccgggagt gattcaggca tcacttcaac gcttttaatg
     1321 gtaaaagttc cccctttgtt cagggtgact tctcgcatca ggttgcgaat ttttctctca
     1381 atgttaagcg tatttttctg gcagtcggcc gtgtaaaact cgctgatagg taacgtaacg
     1441 gtgagatcga tctcctgggg ttcaatgcca ctgttcagta atgcgtggtg aactgccaac
     1501 acatttgtat cagagtactg atactcaata tgagtcgtgc tgatcgcatc ttcgctgacc
     1561 gggtcgtagg tatatttacg gccatcaagc agatagttat aagtacgact gctgcctaag
     1621 ccctcgactt tccagttatg gcgaaaagag ttcactgata ccgcactttt cagcgtcttg
     1681 ccttcaaacc aggcaagttt cacatttgta gaaccatcat cgcaaaaaat tctcatacca
     1741 tctccattta tactcaactt gagtattcat ctttacacaa taactactca cattaagtaa
     1801 atacgctcat aaaccacagg taagtactca agctgcgtac ttcacttggg cagttaacta
     1861 ctcaatttga gcattatatc atttatcttg cagcaatcta gcatttatct agcataaatc
     1921 ttgctgcaag attgcacaca aaaaaagaat gcagcaagat tgctgcatcc cggattaata
     1981 gccctgtttt tccttatatg cctcaactgc tttttccaga acttccacca gtttcaaccc
     2041 ctcacgctct gcgatatccc ggatctcttg gtcgaaagca ggtgtgacac gtgttgcaaa
     2101 tgccagcgtc ctgtttgttt ttctggcaga acggccatcg cggcgggtat atacctcggt
     2161 cactgtttgc gtaaccacag gctcatggtg cactggcgat gagtctgcct gttgtctctg
     2221 aacatcagtg gcagcgacag ccggagctat ctcaggcgca ttcaagctgg tagccacttc
     2281 gtcggggccc gggggtaccc ccaacccact acgggttctt ttagttctaa gttttgacgc
     2341 atcagccatt actcttctcc aatcttatcg atgagtgcct ggataagttc atcagctctc
     2401 tcgtttaaac ctttataacg tgtttctgtc acagcgagtc ctgaattcat cgcttttctg
     2461 tatgctggct tctcaaaaag gcatccagat aaagtttcat aacctgcttc actgatatat
     2521 gcacgagcat ccagctcttc cgcttcggta cctacccggc ttaaagcgaa gaccagtttt
     2581 gaacgtggta taccttcttt taccaacgcg ttgaaaactt taattgccgg gacaagatcg
     2641 tcaagggagg ctcccgttgg ctggacgata aggtcggcga ccttagcaat ttctgaagta
     2701 cctttacttg cacgagcagg gccatcaaga atcagaaggt caaaatcgcc ggcagattct
     2761 atcgcctgct ttgctgttga aaacacttca acggaagcaa ccggtgacaa accagctgac
     2821 agacgtctac gatgccactc agcactcgtt gcctgttcca agtcgagatc agctaatttt
     2881 gttttgatac cacaggcgga tgcttcacgc gctaacgctc tcgctttagt gcttttgccc
     2941 acaccacctt tctgagaaat aaaaccaact atccaggcca tttagccccc tttacttgct
     3001 gctatcttgc agcaaacatc aatataaacc agattgagat taggtagagt aactcttcca
     3061 gaacaagata acagcttgca gcaagattgc aacaataaat aatcttgcta cttagcagag
     3121 tgctggaatt gacagccgaa tacttgtgtc acgttaacaa taaaagaaac ctcattcaga
     3181 atcgcatcga cagatgaagc aagattgccg caagattgca gatagcataa cataaacaga
     3241 gcgctaggcc gtatgtgtac agcatgatag ctgcaatcta ttttattgct gctatcatgc
     3301 tgaaatatca ttattagaat caggcagccc tcgccgcagc catgtaactg tgcgtagcga
     3361 gcgaactggc gaggaagcgt tccggcagat ggctcttacg ctcggcacaa gaagagtttc
     3421 ccatccaggc aagtactatg ggttgtcagc gccagagata taagactgtt ctggcctgag
     3481 aacgtggcct ctatgagttt ggtgctctat cttccataga tgaatgcacc agctccccct
     3541 tgactgaacc gcgaatacgg ctttttaacg cctctcaagt tgttttatca gcgctaccgc
     3601 atcggcttcc ggcatggcga actgcacacg atgacgcgcc gcgacatcca gcgcaaagac
     3661 cttcgtgtag acctccgttg agctgatgga cttatgcccc atcagactct gcaggacctt
     3721 cagcggtata ccggcataca gcatgtgcat cgcataggaa tggcggaacg tatgtggcgt
     3781 gaccgggaca gagaacgtca ctccatcagc ggcagcggcg gcaaccgcct ccccaatcca
     3841 ggtcctgacc gttctgtccg tcacttccca gatccgcgct ttctctgtcc tgcctgttcg
     3901 tttattacgc cgttccatgg ggattttcag tgtggccacc atcgtctgca gctggctgac
     3961 gtaccaggcg tcagagagcg gaaccagccg gtgagtctgc tgaccggcgg gcattcttcc
     4021 tgccgtcctg gcggcttttt cggtccgctg cttcagagtg gccaactgca caaacggata
     4081 cggaggcgca agcgaaaaat ccccccgcgt cagcgccagt gcttcattaa tgcgcgcacc
     4141 ggtgttccac agtgtggcca gcagcat
//
LOCUS       DAJOSY010000200         4161 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJOSY010000200 DAJOSY010000000
VERSION     DAJOSY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.200"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..759
                     /locus_tag="OHN27_004282"
     CDS             <1..759
                     /locus_tag="OHN27_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCQ2288610.1"
                     /translation="VEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISKEGTTEWCAE
                     YDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKG
                     GWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKALTKWWNDTA
                     SQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDGKKPDRCDVL
                     QELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            780..1622
                     /locus_tag="OHN27_004283"
     CDS             780..1622
                     /locus_tag="OHN27_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCQ2288611.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIKAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1650..2608
                     /locus_tag="OHN27_004284"
                     /pseudo
     CDS             1650..2608
                     /locus_tag="OHN27_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            2620..3081
                     /locus_tag="OHN27_004285"
     CDS             2620..3081
                     /locus_tag="OHN27_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCQ2288612.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKEDP"
     gene            3788..>4161
                     /locus_tag="OHN27_004286"
     CDS             3788..>4161
                     /locus_tag="OHN27_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288613.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKN"
BASE COUNT         1358 a          753 c          935 g         1115 t
ORIGIN      
        1 gtggagcaga tgcaaaacca gatggacccg gtgtacacgc cggcgcgaaa aatccacctg
       61 taccactgcg accatcgcgg cctgccgctg gcccttatca gcaaggaagg gacaacagaa
      121 tggtgcgcag aatacgatga atggggcaac ctgctgaatg aagagaaccc gcatcagctg
      181 cagcagctta tccgcctgcc ggggcagcag tatgatgagg agtccggcct gtattacaac
      241 cgccaccgct attatgaccc gctgcagggg cgatatatca ctcaggatcc gattgggctg
      301 aaggggggat ggaattttta tcagtatccg ttgaatccag ttacgaatac agatcctctg
      361 gggttagaag tttttcctag accattcccc ttgccaattc catggcccaa aagccctgca
      421 cagcagcaag cagatgataa tgctgcaaaa gcattgacaa aatggtggaa cgatacagca
      481 tcacaaagaa tatttgactc tctaatattg aataatccgg gactagcatt agatataaca
      541 atgatagctt ctcgtggaaa tgttgcagac acagggataa ctgatcgtgt caatgacata
      601 ataaatgaca gattctggag tgatgggaaa aaacccgaca gatgtgacgt acttcaggaa
      661 ctaattgatt gtggtgatat tagtgctaaa gatgcaaaaa gcacacagaa agcctggaat
      721 tgtcgtcact ccagacagtc aaacgataaa aaaagatagc ccttgtggag gttcctgcaa
      781 tgtcaaatac ataccagaaa agaaaggcaa gtaaagagta tggtttatat aatcaatgta
      841 agaaactaaa tgatgatgaa ttatttcgct tacttgatga tcacaattcc ttgaaaagga
      901 tttcatctgc cagagtatta cagttaagag gtgggcaaga cgctgttaga ttggcaattg
      961 agttctgctc tgataaaaat tatatccgta gagatatcgg agcatttata ctcgggcaaa
     1021 taaaaatttg caaaaaatgc gaagataatg tttttaatat tttgaacaat atggcattga
     1081 atgataagag cgcttgcgtt cgagctacgg caatcgagtc aacggctcag cgatgcaaga
     1141 aaaacccaat ttattcacct aaaatagtag aacaatctca aattactgct tttgataaat
     1201 cgactaatgt cagacgtgct acagcatttg ctatttctgt tatcaatgat aaagcaacaa
     1261 ttccactatt gattaatctg ttaaaagatc caaatggaga tgtcagaaac tgggccgcat
     1321 ttgcaataaa tatcaataaa tatgataata gtgatattag ggattgtttt gtggagatgc
     1381 ttcaggataa aaatgaggaa gtccgtatta aagcaataat cggactttcc tacagaaaag
     1441 ataaaagggt tttatctgtt ttatgcgatg agttaaaaaa aaatactgtt tatgatgata
     1501 tcattgaagc tgcgggtgaa ttaggtgata aaacgctact tcctgtttta gatactatgt
     1561 tgtacaagtt tgatgataat gaaattataa cttccgctat tgataagctg aagcgttcat
     1621 agcgtgagtt gcctatgcac agtgggggat tcccgccggc acggtgcaga tgctcgaccg
     1681 gctggaaagt gaaatcctgg ctgaccgggt gagtgaggaa agccgccgct ggctggcatc
     1741 gtgcggcctg accgtggagc agatgaaaaa ccagatggac ccggtgtaca cgccggcgcg
     1801 aaaaatccac ctgtaccact gcgaccatcg cggcctgccg ctggcgctca tcagcacgga
     1861 aggggcaaca gcgtggtgcg cagaatatga tgaatggggc aacctgctga gtgatgagaa
     1921 cccgcatcat ctgcagcagc tcattcgcct gccgggtcag cagtatgatg aggagtccgg
     1981 cctgtattac aaccgccacc gctattatga cccgctgcag gggcggtata tcactcagga
     2041 tccgattggg ctgaaggggg gatggaattt ttatcagtat ccgttgaatc cggtcataaa
     2101 tgtagatccg caaggtttgg ttgatataaa tttatacccc gaaagtgatc ttatccattc
     2161 tgtagctgat gagattaata tcccaggcgt tttcacaatc ggggggcatg gtacccccac
     2221 atctattgaa tccgcaacgc gcagtatcat gacagctaaa gatctagcat atctaattaa
     2281 atttgatggg aattataaag atgggatgac agtttggtta ttttcttgta atacaggtaa
     2341 aggacaaaat tcatttgcta gccaattagc taaagagtta catacaaatg taataggacc
     2401 tgacacgcta tggacgtggt gggggcgagg aactaatggt aagttaaaaa tggatacagt
     2461 gctaacagca ccaacgaacc ttaattcaaa taaggatcta atggctataa caacaaaaga
     2521 ccttggtaat tggataacat atgggccatc tgggcacccc atttctaata tgcaaggtac
     2581 gccagaaaaa cccagtgata taagataggt tgtagatgta tgaaagcatg cttgttacta
     2641 tttttttatt tttctcttat tggtcaattg catggtgctg atgtgaaaat aaaagaaaac
     2701 gaaagtgtga tgggatctac agcaatgacc tatgatttaa gtgaagaaaa gctgatgaaa
     2761 ctgaagtata aatcacaaca tggtgattca gaggcatcat ttcgactata tcaatattac
     2821 tgctttacta aaaataatat tgataaacaa ttgcgattct tggaaagatc agcatctcag
     2881 gggaatgtta cagcgcagtt taattatggg gtctttttat cagatacaaa tccaacatta
     2941 tcagaatatt ataatttgaa tagagctatt tattggatgg aattcgctgt aaataacggt
     3001 aatattgatg caaagagcaa acttcaagag ctcaaaaagc taaagcgaat ggatagaagg
     3061 aagaataagg aggatccgta aaagcaatag ttcatcagac tgccgcagca gtagtatgat
     3121 gatgaatcag ggctgtacta taactattat cggtaacatt aaccgcagca ggggcggtat
     3181 aacactcagg ctccgattgg gctggaaggt aggtggaatt tttatcagta tccgttgaac
     3241 ccgatttctg gaatcgatcc ccttgggatt agccacctgt ttatacagca taactttagg
     3301 tatgctttcg tgtgtttcag atataccatg tgatgataat ttttatgatg tgcttagtat
     3361 tccagtcgct tctggtaata atatgcaatg taaaaataat ccccgttgta cacatttata
     3421 aatagatggc caatacctca aggcctatgg agttggaatg ttaatgaccc gggagcaagc
     3481 aatagaaaac ctgacggcat acgattagta ccgtcagtta atacaggaac ttataatcgc
     3541 aatggatttt caatacatag ttgtttaaac gcttttggtc cgtctttagg acctcgattc
     3601 tgttccgagg gttgtataac tggtttatct aatgacatgc aaaaattaaa tgagttaata
     3661 ttttcagagc cagatagcac attaacagtt acggactaaa aaatatggca aggttattaa
     3721 caattgtatt tttttctcta tttctgcgag tgcagttact gcaagacaat ttcagagaga
     3781 gttagatttg gcagacaaag caatattatg ggcactaata tcagccagta ctaaagaggg
     3841 acgaaaagcg tgttcactaa gctattttgc ctgtaaggcc gcagaggctg aactcggact
     3901 ggcatatatg gctgcgaatg ataacaagga atttctcaca tccttatcta atataatgag
     3961 gtacaaaata gatgctggac tttccgaatc ctatacatgc tatttattaa gtaagggaaa
     4021 gatcataaga ccatatctga aaaatctaaa tccactccaa cttgccgctg attgcattga
     4081 aacagtaaat aaaataaaag ataagaataa aaaaatcatt gatataaatt cagttaatat
     4141 ttgtagtgat gataaaaata t
//
LOCUS       DAJOSY010000201         4147 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJOSY010000201 DAJOSY010000000
VERSION     DAJOSY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.201"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(37..822)
                     /locus_tag="OHN27_004287"
     CDS             complement(37..822)
                     /locus_tag="OHN27_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2288614.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIITGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            1141..2418
                     /locus_tag="OHN27_004288"
     CDS             1141..2418
                     /locus_tag="OHN27_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2288615.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQIAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLTLTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            2445..3923
                     /locus_tag="OHN27_004289"
     CDS             2445..3923
                     /locus_tag="OHN27_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCQ2288616.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDNMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         1093 a          946 c         1003 g         1105 t
ORIGIN      
        1 tcattaataa gagaaaacag aaaccaccgt tatgggttag cgcactaaat aaccgccatc
       61 aaccactaat aaatgcccgt tgacataatt cgatgccgga cttgcgagga atacggctgc
      121 gcccattaaa tcctgagtat cgccccaacg gtttgccgga atatgatcaa gaacgcgctg
      181 attggtttct ggattactgc gtgtcgccag cgtaatatct gttgcataat agccaggggc
      241 gataccattt acctgaatat tatattgacc tagttcatca caataagctt tggtgaaccc
      301 ggcaagagca tgtttagtgg cagaatatgc aggtgaccat tgtccaccta agtaagagaa
      361 caatgaacag atattaataa ttttgccgct tttctgcggg atcataattt ttgcagcttc
      421 atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc atcggatccc agtcggcacg
      481 accgaagtcc agcaccttat tcagcttaca aataccggca ttgttaacca gaatatcaac
      541 tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc ggcgcgcctt ctgcggtgat
      601 atccacctgc atgaagtcca cctcaacacc ctgtttttca atcatttcct ttgtttcgcc
      661 gttatctttg acgaaactag gaataaagat atttgcgcca gctttggcca acgccatggc
      721 aaatgcctgg cctaaaccgc tattcccacc ggtaataatt gcggttttac ctttcaggga
      781 gaaaaaatcc attgagaacg cattgagaga ttcgattgac atagttagct ccaatttccg
      841 taaggcaaaa aaaaagaaag gtaacttccc ccgcgagagg gaagttacct ttctcattat
      901 ctctggtaac tggagataat ttttagcata agggcataac tgctaatgtg atcgtaatca
      961 cagtgtgata ttcgtattgt caaataattt taaaaagcag aagcgtgatt actctcacac
     1021 aacacctctg ctttccatgt tatttattat ttcaattact agagacctga gaaaagtaat
     1081 ccacctgaaa gggcggctta cttttctctt ttttttgtct attacaaatg ggaatacgat
     1141 atgcaacaca actcatatcg ccgttggata accctcgcga taattagttt tagcggcggc
     1201 gttagtttcg acctggctta tttacgttat atttatcaaa ttcccatggc gaaatttatg
     1261 ggattcagca ataccgagat aggtttaata atgagtacct ttggtattgc ggccattatt
     1321 ctttatgccc ccagcggcgt tattgccgat aaattttcac accgcaaaat gattacttcc
     1381 gcgatgatca ttaccggatt actgggtctg ttaatggcaa cgtatccacc gctgtgggta
     1441 atgctctgta ttcagatcgc ctttgcgata acgacgattt taatgctgtg gtcggtgtcg
     1501 attaaagctg catcgttgct tggcgatcat agcgagcaag ggaaaattat gggctggatg
     1561 gaagggctgc gcggcgtcgg tgtaatgtcg ctggcggtgt ttaccatgtg ggtcttttct
     1621 cgctttgcac cggatgacag caccagcctg aaaacggtca ttatcatcta tagtgtggtt
     1681 tacatcttgt tggggattct gtgctggttt tttgttagcg ataacaacaa cctgcgcagt
     1741 gccaataatg aagaaaaaca gtcattccag cttagcgaca tcctggccgt tttgcgtatc
     1801 agcaccacct ggtattgcag catggtgatt tttggcgtct tcaccatcta cgccattctg
     1861 agttactcca ccaactatct gaccgaaatg tatggcatgt cgctggtggc ggcgagctac
     1921 atggggattg tgatcaacaa aatattccgc gcgctgtgcg gcccacttgg cggcataatc
     1981 accacctaca gcaaagtgaa atcccctacc cgcgtgatcc aaatcctttc cgtactcggc
     2041 ctgctgacgt taactgccct gctcgtcacg aactctaacc cgcaatcggt cgcgatgggg
     2101 attggcctga ttttactgct gggattcacc tgttacgcct cacgcgggct gtactgggcc
     2161 tgccctggcg aagcgagaac accgtcttac attatgggca ccacggtagg tatttgttcg
     2221 gtgattggat tcctgccgga tgtcttcgtt tacccaatta tcggccactg gcaagacacc
     2281 ctgcccgctg cagaagccta ccgcaatatg tggctgatgg gcatggcggc gcttggcatg
     2341 gtgattgtct ttaccttttt gctgttccaa aaaattcgta ctgctgatag cgcccccgca
     2401 atggctagca gcaagtaagc caggaaaatt acaggagaaa aatcatgtcg aaaaaataca
     2461 tcatagggat tgatggcgga agtcagagca caaaagtggt gatgtacgat ctggaaggta
     2521 acgtggtttg cgaaggcaaa ggcttattac agccgatgca cacgccagat gccgatactg
     2581 cagaacatcc tgacgacgat ttatgggcat cattatgttt tgccggtcac gatttgatga
     2641 gtcagtttgc cgggaataaa gaagatattg tcggtattgg tctgggatcc atccgttgct
     2701 gccgtgcgtt attgaaagcc gatggcacgc ctgctgcgcc gttgattagc tggcaggatg
     2761 cacgcgttac acgcccttac gaacacacta accctgacgt ggcatatgtc acctcttttt
     2821 cgggttatct gacgcatcgc ttaaccggcg agtttaaaga caatatcgcc aactattttg
     2881 gtcagtggcc ggtggattat aagagctggg catggagcga agatgctgcg gtaatggata
     2941 agtttaatat cccccgtcat atgctgtttg atgtgcaaat gcctggcacc gtcctcggac
     3001 atatcacacc acaagccgca ctggcgacac atttcccggc aggactgccg gttgtttgta
     3061 ccaccagtga taaaccggta gaagctctgg gggccggatt actggatgat gaaacggcgg
     3121 taatttctct tggcacttac atcgcattga tgatgaacgg caaagcactg ccgaaagatc
     3181 cagtagcgta ctggccgatt atgtcttcta ttccgcaaac attgctgtat gaaggttacg
     3241 gtattcgcaa aggcatgtgg acggtgagct ggctgcgcga catgctaggc gagtcgttaa
     3301 ttcaggatgc cagggcgcag gatctttcac cggaagattt actcaacaaa aaagcttctt
     3361 gtgtgccgcc aggctgtaat ggtctgatga cggtgctgga ctggctgacc aatccgtggg
     3421 aaccgtacaa acgcgggatt atgatcggct ttgattccag catggattac gcatggatat
     3481 atcgttcgat actggaaagc gtggcgctga cgctgaagaa caattacgac aatatgtgta
     3541 atgaaatgaa tcactttgcg aagcatgtga tcattactgg cggcggttcg aacagcgatc
     3601 tgtttatgca gatttttgcc gacgtgttca accttccggc acgacgtaac gccattaacg
     3661 gttgtgcaag cctgggggca gcgattaata cagcagtagg tctggggcta tacccggatt
     3721 acgcaacggc tgtcgataac atggttcgcg tgaaagatat ctttataccg attgagagca
     3781 atgccaaacg ctacgacgcg atgaataaag gcattttcaa agacctaacc aaacatactg
     3841 atgtgatcct gaaaaaatcg tatgaagtga tgcatgggga attggggaat gtggattcga
     3901 tccagagctg gtcgaatgcg taagtgagga aggccgggcg ggaaactgcc cggcctgaac
     3961 atacctgaat ggttatcccc gctgacgcgg ggaacataag ttcgacaact taaaaaatga
     4021 taacaagtcc ggtttatccc cgctgatgca gggaacataa taaaaacttt aactagttcg
     4081 gtttatccct gtttttacaa ggaatattaa ctacactcga tgtctttaga ataaaagaca
     4141 atacgaa
//
LOCUS       DAJOSY010000202         4092 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJOSY010000202 DAJOSY010000000
VERSION     DAJOSY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.202"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..771)
                     /locus_tag="OHN27_004290"
     CDS             complement(<1..771)
                     /locus_tag="OHN27_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288617.1"
                     /translation="MVYENELWHSFLLRSQIMYNLSNCRNIISKHGALRYDAFPRFEL
                     IDMYKLHSLQDIYDILATRNGYILTSNIVSLSSGVYGYFNAVEDACRKNFHITNSYPL
                     VNISNIRYCHKCIVEDIHSKGIGYLRHRWLFESKCAVHSTSLYEVCFDNYLNAVKGLS
                     DLIISGINPHGYCSLVVDVSNPFKNPVYILPCARNKILKWISDNKNMLIYFLFDLFKC
                     KSHSSLSKVIEVKIMHDRYISAVFKMLSEVNFCNFNSFI"
     gene            complement(783..2339)
                     /locus_tag="OHN27_004291"
     CDS             complement(783..2339)
                     /locus_tag="OHN27_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCQ2288618.1"
                     /translation="MRMLPVLPDESLFSRFCRTTTVYGMSPSSLLTIIFNKPDMNVHP
                     ILNSGLKAISLHTSESADQLWHEQTLLPLFAWALPISRNEIMDFNTTPARLNRLCRLS
                     NFSLGQRTLLKFCPVCAREDTFHYGVTYWHLAHQLHGVTTCHRHPVALESIHVPSSPH
                     IRIGLMPPVSYTEQLSNEIDFDFAKFCYESLNIIRRKDITHPNYMDVLKKLNLLSLDG
                     NLKKNVFYAHVYAKCQLFGEGSSGLIPTSLTDYHYWEPILKDKCCQHPTKHLLLCYCL
                     LNTCWPTYAGSRTNKKKEIFKSHKKYSFHIVENNTSVSNLGKEFSRSRCYIKTLIYKK
                     YLRAFKRNTKINIFTELLIKSMAVRGFSLASIAEKNSLSEGAVSSVISSCYGLCSWRK
                     KCKKDSLRRRHKQKILRFIHNQSVSITRKLVKESCYASFYWLNKHECDWLNSCLPKTI
                     RCYKNKRVDWSERDIISSSLINDVLSQGQYSMSLTSLDALLGGHGWLLKYRDKLPMTM
                     ILLRKMELIK"
     gene            complement(2380..3828)
                     /locus_tag="OHN27_004292"
     CDS             complement(2380..3828)
                     /locus_tag="OHN27_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCQ2288619.1"
                     /translation="MNLIRSVSAIYKEQELPEYRGNPLIEALPEALTEDEVLLEMSYF
                     PEIDEKIRWTAPANVREQYVERIKKFRCPQTNLIQAYKMILRALRESYAARNPLKSGT
                     IQYLHYYGNERPDIEPESGYFKSQAETITIVGMSGSGKTTMIEQVMDHFPQIIEHSSY
                     KGVFPGFSKQIVWVKINCPYNSSVRDLCEEILQKLDDAIGIERTTPEIRNGALARQIA
                     QRIKSSFLGILVIDEMQRLKFSRTGGESKLIDFLHEIVDSMGVSMVFCGNHPFDETLT
                     KKMRIARRAESGGYMKIKNVRYDSQDWQSFIHYLWPLQWTNVETPLNDELNEKLFILS
                     KGNIGLAQMIYRGAQLKVIGSGNEIITGAVLSASVPVLVSHTEEYKDTPLPGAATEDE
                     EAKWLSASNEGDASAKIMAEKSLKSLIPGDIDRPQHKEFVGKIDEVLRNFDEKILSLD
                     HDLVINRTAEKKNTYDALQRCGLINIDPLNKL"
     gene            complement(3828..>4092)
                     /locus_tag="OHN27_004293"
     CDS             complement(3828..>4092)
                     /locus_tag="OHN27_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288620.1"
                     /translation="SLSNINNKDEFNRKGNERLNEIYESRRVHGKDIKKNRKNELDSL
                     GRTHVANGRSEPLLPSSSNVLLLPGREEKQRWLKGKKKTEDAE"
BASE COUNT         1237 a          838 c          708 g         1309 t
ORIGIN      
        1 aataaatgag ttgaaattac aaaaattaac ttcacttaac atcttgaaca ccgcagaaat
       61 atatctatca tgcattatct tgacttcgat aaccttagat aaacttgaat gcgacttaca
      121 tttgaagaga tcaaagagaa agtaaatcag catattttta ttgtccgaaa tccattttaa
      181 aattttatta cgtgcacatg gtaatatata cactggattt ttgaaagggt ttgaaacgtc
      241 aaccacaagg gaacaataac catgaggatt tattccactt ataattaagt cactaagtcc
      301 tttcactgca ttcaaatagt tatcaaaaca aacctcatat aaactggtac tatgaactgc
      361 acatttagat tcaaacaacc atctatgacg cagataacca atgcctttag agtgtatatc
      421 ttcaacaata catttgtgac agtaacgaat gtttgagatg tttaccaatg gatatgaatt
      481 agttatgtga aaatttttcc gacaggcatc ttcaacagcg ttaaaatacc cgtaaacacc
      541 agaagataac gacactatat tggaagttag tatataacca tttctagtgg caagaatgtc
      601 atatatatcc tgcaagctat gcaatttgta catatcaatc agttcgaaac gaggaaaggc
      661 atcgtacctt agtgcgccat gctttgatat tatgttcctg caattggata agttatacat
      721 tatctgtgaa cgcagaagaa aactgtgcca caattcattt tcgtaaacca tataactccc
      781 tcttatttaa ttagttccat ttttctgaga agtatcattg tcattggtag tttatctctg
      841 tatttcaaaa gccagccatg ccctcccagt aaagcatcta gacttgtaag tgacatcgag
      901 tactgcccct gagataaaac atcatttatt aatgatgatg agataatatc tctctcgctc
      961 cagtctactc ttttattttt atagcatcgt attgttttag gaagacagga attaagccag
     1021 tcacattcat gtttatttag ccaatagaaa cttgcataac aactttcttt gactaacttt
     1081 cgtgttatag aaacggattg attatgtata aatcttaata ttttctgctt atgacgccgt
     1141 cttaaagaat cttttttaca ttttttacgc catgagcata aaccgtaaca agatgaaatt
     1201 acagaggata cagctccttc cgataatgag tttttctcag ctatggatgc cagactaaac
     1261 ccccttacag ccatagactt gataagcaat tcagtgaata tattaatttt tgtgtttcgc
     1321 ttaaacgccc tcaggtattt tttataaata agtgttttaa tgtaacatct gctgcgacta
     1381 aattccttcc caaggttgct aacactagta ttattttcaa ctatatgaaa actgtatttc
     1441 ttatgacttt taaagatttc tttcttttta ttagtacgac ttcctgcata cgttggccag
     1501 caagtattta acaaacaata acaaagcaaa agatgctttg tgggatgctg acaacatttg
     1561 tctttgagta taggctccca gtaatgataa tcagttaggg atgttggtat aagtcccgat
     1621 gaaccctccc caaataactg gcacttagca taaacatgtg cgtagaatac atttttcttt
     1681 aaatttccat ccaatgataa taaattcaac tttttaagta catccatgta attggggtgt
     1741 gtaatatctt ttcttctgat tatatttagg gactcataac aaaacttagc aaaatcgaag
     1801 tctatctcat tgctaagttg ttctgtatac gaaacaggag gcatcagtcc aatacgtatg
     1861 tgcggtgaag aagggacatg gatgctttca agcgctaccg gatgccgatg gcaggttgta
     1921 accccatgaa gctgatgcgc cagatgccaa taagtaacac cataatgaaa agtatcttca
     1981 cgagcgcaaa ccgggcaaaa cttcaatagt gtgcgctgcc ctagtgaaaa attactaagg
     2041 cggcacaatc gattaagcct ggcaggggtc gtgttgaaat ccataatttc attacgactg
     2101 attggtagtg cccaggcaaa aagagggagt aaagtctgtt catgccagag ctgatctgca
     2161 ctttcggatg tatgaagaga aatagccttt aatcctgaat tgagaattgg atggacgttc
     2221 atatcaggtt tgttgaaaat gatcgttaac agagaagatg gggacatacc gtacacggta
     2281 gttgtccgac aaaaccggct gaacaaggat tcatcaggca aaacaggcaa cattctcatt
     2341 tcccacacct ctctttgacc gttacgaact gttacctcat tacaatttat taagtggatc
     2401 tatatttata agcccgcaac gctgaagagc atcgtaggta tttttctttt cagctgttct
     2461 gttgataaca agatcgtggt caagagacag tattttctcg tcgaaattgc gcaaaacctc
     2521 gtctattttt cctacgaatt ccttgtgctg tggcctgtct atatccccag ggatcaaaga
     2581 ctttagggac ttctctgcca tgattttagc cgaagcatca ccctcattag atgcagagag
     2641 ccatttagct tcctcatctt cagtcgcggc ccccgggagt ggagtatcct tatattcctc
     2701 cgtatgactc acgagtaccg gtacagaggc cgacaagact gcgccagtga tgatttcatt
     2761 gccgctaccg ataaccttta attgtgcccc ccgataaatc atctgagcca atccaatatt
     2821 gcctttagat aaaatgaata atttttcgtt taactcatca tttaatggag tttcaacatt
     2881 tgtccattgt aatggccata aataatgaat aaacgattgc cagtcttgtg aatcgtatct
     2941 aacattctta attttcatgt aaccaccaga ctctgcccgc ctggcaattc tcattttctt
     3001 tgtcagcgtc tcgtcaaaag gatgatttcc acaaaaaacc atagataccc ccatggaatc
     3061 tacaatttca tgtaaaaaat caatcaactt actctcaccc ccggttcttg aaaatttaag
     3121 cctctgcatt tcatcaataa caaggatacc caaaaatgat gatttaattc tttgtgcaat
     3181 ctggcgagcc agagcaccat ttctaatctc aggtgtagtt cgttcaatac caattgcatc
     3241 atctaatttt tgtaaaatct cttcacaaag gtctctcaca cttgaattgt atggacaatt
     3301 aatctttacc catacaattt gcttactgaa gccgggaaaa actcctttat agctgctgtg
     3361 ttctataatc tgagggaaat gatccatgac ttgctcaatc atggtagttt ttccagaacc
     3421 actcattcca actatcgtaa tagtttcagc ctgggattta aaatatccac tttctggctc
     3481 aatatcagga cgctcatttc catagtaatg aagatattga attgttccgc tttttaaagg
     3541 attacgagct gcatagcttt ccctaagtgc ccgtaagatc atcttataag cctggataag
     3601 attggtttga gggcaacgga atttctttat acgttcgaca tactgctctc taacattcgc
     3661 cggggcagtc cagcggattt tttcgtcaat ttcggggaaa taactcattt ccagaagtac
     3721 ttcatcttcc gtaagcgctt caggaagtgc ttcaattaag ggattgccac gatactcagg
     3781 taactcctgt tctttatata tcgctgaaac gctccgtata aggttcatta ttctgcgtcc
     3841 tcagtcttct tcttaccttt taaccatctt tgtttctcct cccggccagg caaaagcaga
     3901 acattactgg atgaaggaag taggggttca gatcttccat tagcaacatg agttcgccct
     3961 aatgaatcaa gttcattttt cctgttcttt ttaatatctt taccatgtac ccggcgagat
     4021 tcgtatattt catttaatct ttcgttacct ttcctgttaa attcatcttt attattgata
     4081 ttgctgagcg aa
//
LOCUS       DAJOSY010000203         4088 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJOSY010000203 DAJOSY010000000
VERSION     DAJOSY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.203"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            53..1711
                     /gene="dpiB"
                     /locus_tag="OHN27_004294"
     CDS             53..1711
                     /gene="dpiB"
                     /locus_tag="OHN27_004294"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCQ2288621.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIVEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1680..2360
                     /gene="dpiA"
                     /locus_tag="OHN27_004295"
                     /gene_synonym="citB"
     CDS             1680..2360
                     /gene="dpiA"
                     /locus_tag="OHN27_004295"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCQ2288622.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2401..3786)
                     /gene="dcuC"
                     /locus_tag="OHN27_004296"
     CDS             complement(2401..3786)
                     /gene="dcuC"
                     /locus_tag="OHN27_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCQ2288623.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1126 a          957 c         1052 g          953 t
ORIGIN      
        1 acattttaat tacatccgtc aaagaggctc gggacaaccc gcaaggaaaa caatgttgca
       61 gcttaacgag aataaacagt ttgcattttt ccaaagactg gcatttccgc tgcgtatctt
      121 tttgctgatt ctggtgttct caatatttgt cattgcagcc ctggcgcaat attttacggc
      181 cagttttgag gactatttaa cgcttcatgt acgcgacatg gcaatgaatc aggcgaaaat
      241 tattgcctcc aatgacagtg tcatctctgc ggtgaaaacg cgtgactaca aacggctggc
      301 gaccatcgct aacaaattac aaagagatac cgattttgat tatgtggtga ttggggaccg
      361 gcactcgatc cgcctttacc atcctaatcc ggagaaaatt ggttatccta tgcagttcac
      421 caaacagggc gcgctggaga aaggggagag ctacttcatt accgggaaag ggtcaatggg
      481 gatggcgatg cgcgccaaaa cgccaatctt tgatgacgat ggaaaagtca tcggcgtggt
      541 gtcgattggc tacctggtga gtaaaatcga tagctggcgg gctgagtttt tattaccgat
      601 ggcaggtgtg tttgtcgtgc tgttagggat tctgatgttg ctgtcgtggt tcctggccgc
      661 gcatatccgt cggcagatga tgggcatgga gccaaagcaa atcgcacgcg tggtccgtca
      721 gcaagaggcg ctgtttagtt cggtttatga agggctgatt gcggtggatc cgcatggtta
      781 cattaccgcc atcaatcgta acgcaagaaa gatgctgggg ctgagttccc ccggacggca
      841 atggttgggt aaacccattg ttgaagtggt caggcccgcc gatttcttta ccgaacagat
      901 tgatgaaaaa cgtcaggatg tggtggcgaa ctttaacggt ctgagcgtta ttgccaaccg
      961 ggaagctatt cgttcaggtg atgatttgct gggggccatt atcagctttc gtagtaaaga
     1021 cgaaatttcc accctcaatg cgcaactgac gcaaataaaa caatacgttg agagccttcg
     1081 tacattgcga cacgagcatc tcaattggat gtcgacgctc aatggtctgt tgcagatgaa
     1141 agagtatgat cgcgtgctgg cgatggtgca gggggagtct caggcccagc aacagcttat
     1201 tgacagcctg cgcgaggcgt ttgccgatcg ccaggtggcg gggctgcttt ttggtaaagt
     1261 gcagcgcgcc cgggaactgg ggctaaaaat gatcattgtc cccggtagcc agctttcgca
     1321 actgccgcca ggactggata gcaccgagtt tgcagccatt gtgggcaatt tacttgataa
     1381 cgccttcgaa gccagcctgc gtagcgatga aggaaacaag atcgttgaat tattcctcag
     1441 cgatgaaggc gatgatgtgg tgattgaagt cgccgatcag ggctgcggcg ttccagagtc
     1501 tctacgagac aaaatatttg agcagggggt cagtacgcgt gctgacgagc ccggtgaaca
     1561 tggcattggg ttgtacttga ttgccagcta cgtaacgcgc tgcggtggtg ttatcactct
     1621 cgaagataat gatccctgcg gtaccttatt ttcaatctat attccgaaag tgaaacctaa
     1681 tgacagctcc attaacccta ttgatcgttg aggacgaaac gccgctggca gagatgcatg
     1741 cggaatatat tcgtcacatt cccggattca gtcagatatt actggcggga aatctggcgc
     1801 aggcccgaat gatgatcgag cgttttaagc cggggctaat cttgctcgat aactatcttc
     1861 ctgacggtag agggattaat ttactgcatg aactggtgca ggcgcattat cccggcgacg
     1921 tggtgtttac cactgcagcc agcgatatgg aaacggtgtc tgaagccgta cgttgtggtg
     1981 tatttgatta tctcattaag cccattgcct atgaacggct ggggcaaacg ctaacccgtt
     2041 tccgccagcg taaacatatg ctggaaagta ttgatagcgc cagccagaag caaattgatg
     2101 agatgtttaa tgcttatgct cgcggtgaac ctaaggacga gctaccgacc ggcattgacc
     2161 ccttgacgct aaacgcggtg cgaaaactgt ttaaagagcc tggtgtgcaa catacggcag
     2221 aaacggtggc gcaggcactg accatcagcc gcaccactgc caggcgttat cttgaatatt
     2281 gcgccagccg ccatctgatt attgctgaaa ttgttcacgg caaagttggc agaccacaac
     2341 gcatatacca cagtgggtga catgataaag ccggaggaaa cttgcctccg gcattactat
     2401 ttacttgcct gtgaccgctg ctgccgttcc tggcaccatc agctctgtag caacgataac
     2461 aatcaccaaa ccaacaagca ccggtaccga ggtgcgtttt acgacttcaa acggcgagat
     2521 cttcgccatc ccggcaaccg caacgactac gccagaaacg ggcgaaaggg tacggccaag
     2581 gtttgacgcc tgcagcatcg ggatagtcaa atacgccggg ttaatgccgg aagagtgcgc
     2641 cagtttcggg atcatctcaa caaacgcata aaacggcgca ttgcctgaac cggtcgtgac
     2701 tgccgccagc attgtcagaa tcaccaatac cagcatcagg atgatactcg ccgaaccaaa
     2761 cgaggtagcg atagaaatca gactttgaat aaagccgatg gtgctaagcc cctgagcgaa
     2821 taccccagcg gcaaccagca gcatcaccac gttagcaaac gcatctgcca tcccgcgata
     2881 agccacttcc agaccagaga aaactttctg ggtattaaag ctgcggagga actccagaat
     2941 ggaggcaatc agcatacaaa tcaccagaat agtgatgatg tgtaattgcg gaccccattt
     3001 accgtcaaaa atcagtacac cgatgatcgg cgtgaacggc aaaatggcat aaaacgcagg
     3061 agcagtggtg gtgatttcac tgacatctaa catttcatga gagatgtgct cttttttatc
     3121 cagataacgt tgccagaaga agtgggcgat cgccatgccg ataattgcag caattgagat
     3181 aggcagcgtc gttttgaagg cgaagtcaat cagcgacatt tcggaagctt gcgccgccag
     3241 caccacatcc cctgaagtcg gtgcgagaat aatcgccgcc ggggaggcac aaatggcagc
     3301 agctgcgcca cgactgatac caacgtttac catcaccgga aatagggttg ccatcagcaa
     3361 aacacccaga ccggttgcgg aagagacggc cagagacatc agacaggcga caaaataggc
     3421 ggcaatcatc agcaggtaag gggagttaat atactgcaat ggttttgacg ccagcttgac
     3481 caccatatca ttcgcgccga tatgggtcat gtaagcggca aatccacaca gcatcataat
     3541 catcatgccg aggtcgccgc cgcggctcat tagtaatatt ttaacgtatt caacgatatc
     3601 cgtggcgctg tagcctgttg aagcctggct ggacggtaac actttgtgcc ccataatggc
     3661 actgataatc agcaataaca ggccaccgac aaataacaca ccagtggcgg aatacccttt
     3721 aatgatgtag cgagctacac ccacaataac cacaacccca ataaggagct caatgaatgt
     3781 cagcataatt tttcctgtct ccaggcccca aagtaaataa taaaaaattc ctaaagctta
     3841 aggaaaaaat atgcccaata aattggagat gaatgctgat taaaatcaag aaaaactgcc
     3901 attaagacat tgaagttgct gtttttatac atagataaca aaacgcatat ttagcggttg
     3961 gcaattatca cataataatg tgtgcaaatt gatggtggat tgatgcaaat ttgttaattg
     4021 caatggtgtt agctgcggct gtgcgctcaa aaaacaatct aatatgagca taggttgacg
     4081 atatatat
//
LOCUS       DAJOSY010000204         4034 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJOSY010000204 DAJOSY010000000
VERSION     DAJOSY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.204"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            90..1142
                     /locus_tag="OHN27_004297"
     CDS             90..1142
                     /locus_tag="OHN27_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HCQ2288624.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1688..2695)
                     /locus_tag="OHN27_004298"
                     /pseudo
     CDS             complement(1688..2695)
                     /locus_tag="OHN27_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(3259..3558)
                     /locus_tag="OHN27_004299"
     CDS             complement(3259..3558)
                     /locus_tag="OHN27_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2288625.1"
                     /translation="MNTQYALKTLNQLRPVLIGFRKANGLTQKDVSERLGITQQTYAR
                     LEANPASAGFERLFRVFSVLGVEIVLSSREPLSDAKPEYGAMHNYSSLPARREKW"
BASE COUNT         1062 a          882 c          859 g         1231 t
ORIGIN      
        1 ctttaatgat atctcgtcat tatattaaca ggatgaaatt atcattaatg catatttcaa
       61 tattagcagg gatacctgag gagtttaaag tgaacatata tatcgggtgg cttttcaaat
      121 taatcccttt gattatgggc ttaatttgta tcgcgttagg tggctttgtg ctggaaagtt
      181 cagggcaaag cgagtatttc gtcgcgggtc atgtgctgat ttctctggcg gccatatgcc
      241 tggcattatt cactaccgca tttattatca tttcgcagct cacgcgcggc gttaatacgt
      301 tttacaatac attgttcccc attattggct atgcggggtc aattatcacc atgatatggg
      361 gttgggcact gttagcaggc aatgatgtga tggcagacga gtttgtcgcc ggccatgtta
      421 ttttcggcgt tggtatgatt gccgcctgtg tatcgacggt ggcagcgtca tccggtcact
      481 ttctgctcat tcccaaaaat gcagcgggga gcaagagcga cggaacaccg gtacaggctt
      541 attcttcatt aatcggtaac tgcctcattg ccgttcccgt tttactcacc ctgctcggtt
      601 tcatttggtc tattacgctg ttacgtagtg ctgacataac tccgcattat gtcgcgggtc
      661 acgtattgct tgggttaacc gcaatctgtg cctgtctaat tggccttgtt gccacaattg
      721 tccatcaaac acgtaatacg ttttcaacta aagaacactg gctgtggtgt tattgggtta
      781 tttttctcgg ctcaatcacg gtactgcagg ggatatacgt cttagtcagt tccgatgcaa
      841 gcgcccgact ggctcccggc attattctta tttgcctcgg aatgatctgt tacagcatat
      901 tctcaaaagt ctggctactg gcactggtat ggagacgtac ctgttcgtta gccaacagaa
      961 taccgatgat tcccgtcttc acctgcctgt tttgcctttt cctggcatcg tttcttgcgg
     1021 aaatggcgca gaccgacatg ggatatttta ttccttcgcg ggttttggtc ggtttgggag
     1081 cggtatgctt tacgttgttc tcaatcgttt caatattaga agcaggttct gctaaaaaat
     1141 aattgcaacg taccggataa aaccagcgtt gaccatttgc gtaacgctgg tttttcttag
     1201 gcatcatgaa ataacgcagc attaatgcat agtggttaag gataaaaaaa caacaaagtt
     1261 actgtctttt tcagcctgtt catattttat aaagatctgg ccactgggtg agttttcaat
     1321 cacctttcct tccaccttat attaagcctg gagggtttca gttcacgggc tcattagaaa
     1381 ataatcacaa gatagccact atcaaaatta gctcattttt aatgcggctc taataatttt
     1441 catctttagg taacaggtca ggacgacttc gctgcaattg tgtagagaca ttaaagttgt
     1501 atcctttaac ctgaaaaaga ctttaaagtt gtaaagtttt aaaaaaaaca catacaaaac
     1561 atgatgctaa gtgtgtatca cacgctgttc caacattatg ccttgatgtt cttgcaggaa
     1621 ctgcaattta caggaggaaa aaggtgtttt tgtagttatc tggcgttacg tgacaatgtt
     1681 ggtcagatat ggcataaatc acatttctgg gcgtccccgc gacattcttt tcagcctcag
     1741 cccgaaaaaa attcaacagg ataagcctcc ttatcccgct gccgcgctta ttgatgggga
     1801 tgccattttc cccgtccagg gtgaaattga aggtccactt gggtttctcc ttaaatcgtg
     1861 gagtcagttc actggcgagt tcgggggcca tttcacgtaa tttatccagc gttctctgtg
     1921 cgacatccag gacgctgtcc tgaatctcat tttccagcgc tgtaattttg tcctgcagcg
     1981 cagcctgagc gtcttttacg gcctgctgta aggggttttt agcttcggaa tccccgtcgc
     2041 tgctttccct gtcggctttg aaaatcgcat acgtgggcag caaatcgagg atcttacccc
     2101 ataatgattt tgtgtcggtt ccgaactctt tatcgacatc cagcataatt tccgaacagg
     2161 tatagggggc tgcggcttcc ctgatggcct gacgccataa cgatgcagtg cgtttatccg
     2221 ccacattttt ttcaacttct tttcccaccg ccttgagctc agaaattttc atgcccagta
     2281 aatttctcag gggctcctca tccgggtgga tgcagcggat gcaggtctgc tccggttttc
     2341 ctgaggtcgt tgctttaaac gttttgacga tttcaaaatt gccgtcctca ttcagcagat
     2401 gttctgaggc gagcgtggtc tgcaccctgt cgtcgatcat gataaaatcg ggcaggtcat
     2461 caaattcaca ggctatttca aaacggccgt ccccttctct taggctgaaa cagttcatgt
     2521 cattcttgtc agccttcatt ccttctgttt caaaaaaaat agccagcgct tccagaagcg
     2581 ttgatttccc aaagtcatta cgaccgacaa tacccgtcat gctttcatca ataatgattt
     2641 cagtagagtt tctgtagcct ctgaagtttt tcagttttaa ttttctgagc cgcatatgaa
     2701 gtcctgtgtg taagaaagcg ttttgctgca acctgaatac aaaatatatt ttcaaatgcc
     2761 tgccaacaag cagggataac cgttattcag aatatcttaa cgacatttcg ggattatgat
     2821 ctggtcgcgc tttcagaact ttactgcccc tcaaaacacc ggaaactaac ttgtggttgg
     2881 ttacatggca aagttaaaac tccagaatca cgggtaatga tttcaatgct tattatattt
     2941 tttggaattg actcatgagc tttataattt aaatgataat aataaccatt cttttatgcg
     3001 taatatttgc tactgacact gatgaattta aaagaagata gattttcatt tacggattat
     3061 ttcctaaatt catcgataat gtgatcgaca taaaattttc actgaagaat gaagagataa
     3121 tttaaggccg agcgaaatag ctcaaaatgg tgtatttaac ataacagaag aaatgaaagg
     3181 ctctatcgca acctgatact tcaggccgct aaggggttaa tcttgttgaa gaaaaattag
     3241 accgggaagc gccaggcgtc accatttctc ccgcctggct ggaagtgaag aataattgtg
     3301 catagctccg tattcgggct ttgcatctga caatggttcc ctggatgaaa gtacgatctc
     3361 cactccaagg acgctgaaca ccctaaacaa acgctcaaaa cccgcacttg cagggttggc
     3421 ctctagtcgg gcataagttt gctgtgttat acccaaacgt tcagaaacat ctttttgcgt
     3481 cagtccattg gctttgcgaa agccgattaa aacaggacgt aactgattca aagtcttcaa
     3541 agcgtattga gtgttcataa gtgaccttcc gtatcgacga gttaacaagc ataacgcagg
     3601 gatttacagc atataggctg taatgctcat taacagacta taggctgtaa tacgaatcct
     3661 gccaagatgc ggcatctttc aaatacaagg ccgtattggg taagcagtac accgttatat
     3721 agcacccctt gcccctttgc tggagaggga cccacgtgat cgcaggtctt ttagacgttg
     3781 ttgctaaccg gcatcataca caggtggtaa cccccaggga aactctatga gtgataattt
     3841 attcctgccc cagtcaaaat ataaaacctt atctaactag ctttcttagt attttataaa
     3901 tgaagtgaaa ttagcgaaga agttttacat tgtaaagtga gcaaaaatat ttaccattca
     3961 actggcaaaa tttacaatct gcagccctta gcctgaggaa atgcacatta acagaataat
     4021 catcaagtcc gata
//
LOCUS       DAJOSY010000205         4034 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJOSY010000205 DAJOSY010000000
VERSION     DAJOSY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.205"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            79..1353
                     /locus_tag="OHN27_004300"
     CDS             79..1353
                     /locus_tag="OHN27_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2288626.1"
                     /translation="MFRQDALENRKMKWQGRAILLSGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDVSKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGASVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            1328..3442
                     /locus_tag="OHN27_004301"
     CDS             1328..3442
                     /locus_tag="OHN27_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HCQ2288627.1"
                     /translation="MQQDRSMTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLA
                     MICGHFGKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMP
                     CILHWDFSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFQAE
                     TQQTRISLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGL
                     LTLISAGLMFFILLRAAVSMLRAWSSLVMSTLINVQWQSGLFDHLLRLPLAFFERRKL
                     GDIQSRFDSLDTLRATFTTSVIGLIMDSIMVVGVFVMMLLYGGYLTWIVLCFTTIYIF
                     IRLVTYGNYRQISEECLVREARAASYFMETLYGIATVKIQGMVGIRGAYWLNMKIDAI
                     NSGIKLTRMDLLFGGINTFVTACDQVVILWLGAGLVIDNQMTIGMFVAFSSFRGQFSE
                     RVTSLTSFLLQLKIMSLHNERIADIALHEKEEKKHEIEIVAHMGPISLETNDLSYRYD
                     SQSAPIFSALSLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGRVLINGIDIRQM
                     GINNYHRMIACVMQDDRLFSGSIRENICGFAEEMDEEWMVECARASHIHDVIMNMPMG
                     YETLIGELGEGLSGGQKQRIFIARALYRKPGILFMDEATSALDSESEHFVNVAIKNMN
                     ITRVIIAHRETTLKTVDRVISI"
     gene            complement(3629..3946)
                     /locus_tag="OHN27_004302"
     CDS             complement(3629..3946)
                     /locus_tag="OHN27_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V family bacteriocin"
                     /protein_id="HCQ2288628.1"
                     /translation="MREITLNEMNNVSGAGDVNWVDVGKTVATNGAGVIGGAFGAGLC
                     GPVCAGAFAVGSSAAVAALYDAAGNSNSAKQKPEGLPPEAWNYAEGRMCNWSPNNLSD
                     VCL"
BASE COUNT         1185 a          787 c          943 g         1119 t
ORIGIN      
        1 tataatgcaa attttcgatt tattttccat cattaatcca gataaacaac aaactaatag
       61 tatgcaagga gacattattt gtttcgccag gatgctttag aaaacagaaa aatgaagtgg
      121 cagggacggg caatattact ttccggaata ccactgtggt taatcatgct gggaagcatt
      181 gtgtttatta cggcatttct gatgttcatc attgttggta cctatagccg ccgtgttaat
      241 gtcagtggtg aggtcacaac ctggccaaga gctgtcaata tatattcagg tgtacaggga
      301 tttgttgtca ggcagtttgt tcatgaagga cagttgataa aaaaagggga tcctgtttat
      361 ctgattgacg tcagtaaaag tacacgtagt ggtattgtca ctgataatca tcgtcgggat
      421 atagaaaacc agctggttcg tgtggacaac attatttccc gtctggaaga aagtaaaaaa
      481 ataacgctgg ataccctgga aaaacaacgt ctgcaataca cagatgcgtt tcgtcgctca
      541 tcagatatta tacagcgtgc agaggaaggg ataaaaataa tgaaaaacaa tatggagaat
      601 tacagaaact atcaggcaaa agggctgatt aataaagatc agttaactaa ccaggtggca
      661 ttatattatc agcaacaaaa caatcttctc agcctgagcg gacagaacga acagaatgcc
      721 ctgcagataa ccactctgga gagtcagatt cagactcagg ctgcagattt tgataaccgt
      781 atctaccaga tggaactgca acggtacgag ttacagaaag aactggttaa cactgatgtg
      841 gagggcgaaa tcattatccg ggcgttgact gacgggaaag ttgactccct gagtgtcact
      901 gtcgggcaaa tggtcaatac cggagacagc cttctgcagg ttattcctga gaacattgaa
      961 aattattatc ttattctctg ggtcccgaat gatgctgttc cttatatttc ggctggtgac
     1021 aaagtgaata ttcgttatga agcctttccg gcagaaaaat ttgggcagtt ctctgctacg
     1081 gttaaaacta tatccaggac tcctgcgtca acacaggaaa tgttgaccta taagggagca
     1141 ccacagaata cgccgggcgc ctctgttccc tggtataaag tcattgcgat gcctgaaaag
     1201 cagattatca gatatgacga aaaatacctc cctctggaaa atggaatgaa agccgaaagt
     1261 acactatttc tggaaaaaag gcgtatttac cagtggatgc tttctccttt ctatgacatg
     1321 aaacacagtg caacaggacc gctcaatgac taacgggaat ttcagacaaa ttataaatca
     1381 gcttgatatg cgctggcgac gtcgtgttcc ggttattcat cagacggaga ccgctgaatg
     1441 tggactggcc tgcctggcaa tgatatgcgg tcattttggt aagaatattg acctgatatc
     1501 tcttcgccgg aagtttaatc tctcggcccg tggagcaaac cttgcaggaa tcaatggaat
     1561 agcagagcag ctggggatgg tcacccgggc tctttcactg gagctggatg aacttggtgc
     1621 cctcaaaatg ccgtgtattc tccactggga tttcagtcac ttcgtcgtgc tggtcagcgt
     1681 aaagcgtaac cgttatgtac tgcatgatcc ggccagaggc agaagatatc tcggtcggga
     1741 ggaaatgagc cggtatttta cgggtattgc acttgaggtc tggcccggaa gtgaattcca
     1801 ggcggaaacc cagcagaccc gcataagtct ccgttcactg attaacagta tttacggtat
     1861 taaaagaaca ctggcgaaaa ttttctgtct gtcagttgta attgaagcaa tcaatctggt
     1921 aatgccggtg ggaactcagc tggttatgga tcatgcgatt ccggcggggg acagagggct
     1981 gctaacgctt atttctgctg gcctgatgtt ctttatattg ctcagggccg cggtgagtat
     2041 gctgcgtgca tggtcctcac tggttatgag cacgctcatc aatgtacagt ggcagtcggg
     2101 gctgttcgat catcttctca gactaccgct ggcgtttttt gaacgccgaa aattaggtga
     2161 tatccagtca cgttttgact cccttgacac attgagggcc acatttacca ccagtgtgat
     2221 cgggttaata atggacagca ttatggttgt cggtgttttt gtgatgatgc tgttatacgg
     2281 aggatatctc acctggatag ttctctgctt taccacaatt tacattttta ttcgtctggt
     2341 gacatacggc aattaccgac agatatcaga agaatgtctt gtcagggagg cccgtgccgc
     2401 ctcctatttt atggaaacat tatatggtat tgccacggta aaaatccagg ggatggtcgg
     2461 aattcgggga gcatactggc ttaatatgaa aatagatgcg ataaattcgg gtattaagct
     2521 aaccaggatg gatttgctct tcggaggaat aaataccttt gttaccgcct gtgatcaggt
     2581 tgtaatttta tggctgggag ctggccttgt gatcgataat cagatgacaa taggaatgtt
     2641 tgtagcgttt agttcttttc gtgggcagtt ttcggaaaga gttacctctc tgaccagttt
     2701 tcttcttcag ctaaaaataa tgagtctaca caatgagcgc attgcagata ttgcattaca
     2761 tgaaaaggag gaaaagaaac atgaaattga aatcgttgct catatggggc caatatccct
     2821 ggaaaccaat gatttaagct accggtatga cagtcagtca gctccgatat tcagtgctct
     2881 gagtttatca gtagctccgg gggaaagtgt ggctataact ggtgcttccg gtgcgggaaa
     2941 aaccacatta atgaaagtac tatgtggact atttgaacct gatagcggga gggtactgat
     3001 aaatggtata gatatacgcc aaatgggaat aaataattat caccggatga tagcctgtgt
     3061 tatgcaggat gaccggctat tttcaggctc aattcgtgaa aatatctgtg gttttgcaga
     3121 ggaaatggat gaagagtgga tggtagaatg tgccagagca agtcatattc atgatgttat
     3181 aatgaatatg ccaatgggat atgaaacatt aataggtgaa cttggggaag gtctttctgg
     3241 cggtcaaaaa cagcgtatat ttattgcacg agccttatac cggaaaccag gaatattatt
     3301 tatggatgag gcaaccagtg ctcttgattc agagagtgaa catttcgtga atgttgccat
     3361 aaaaaacatg aatataacta gggtaattat tgcacacaga gaaacaacgt tgaaaactgt
     3421 tgacagagtt atttccattt aaaacctact gacataacca tagaggaatt gcaactgtat
     3481 gaggaatata tcttcctgtg ataattcctc gttatgttca gttgtcagtt ggaggaggaa
     3541 tggaagatag acaacccacc aggaagcata tgtatcattc tgtgtggatt gtccaataac
     3601 agttctttcc tatattaaaa aatactattt ataaacaaac atcactaaga ttatttggac
     3661 tccaattaca cattctacct tcagcgtagt tccatgcttc tggaggtagt ccttctggtt
     3721 tttgtttcgc tgagttggaa tttcctgctg catcatacaa agcagcaaca gcggcagaag
     3781 atccaacagc gaaggcacca gcacaaacag ggccgcacag accagctccg aatgcaccgc
     3841 caatcactcc tgcaccgttt gttgctacag ttttcccgac atcaacccaa ttgacatcac
     3901 cagcaccaga gacattattc atttcattta acgttatttc tctcatatat catttacccc
     3961 tattaaaaaa aatagatatc attaatgcaa tggcgatacc gctaacaaca gattgaacaa
     4021 ggttgctaga attt
//
LOCUS       DAJOSY010000206         4024 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJOSY010000206 DAJOSY010000000
VERSION     DAJOSY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.206"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(119..226)
                     /locus_tag="OHN27_004303"
                     /pseudo
     CDS             complement(119..226)
                     /locus_tag="OHN27_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(244..751)
                     /locus_tag="OHN27_004304"
                     /pseudo
     CDS             complement(244..751)
                     /locus_tag="OHN27_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            792..2306
                     /locus_tag="OHN27_004305"
                     /pseudo
     CDS             792..2306
                     /locus_tag="OHN27_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2383..2673
                     /locus_tag="OHN27_004306"
                     /pseudo
     CDS             2383..2673
                     /locus_tag="OHN27_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2678..2827)
                     /locus_tag="OHN27_004307"
                     /pseudo
     CDS             complement(2678..2827)
                     /locus_tag="OHN27_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2889..3777
                     /locus_tag="OHN27_004308"
                     /pseudo
     CDS             2889..3777
                     /locus_tag="OHN27_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3794..4024
                     /locus_tag="OHN27_004309"
                     /pseudo
     CDS             3794..4024
                     /locus_tag="OHN27_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT         1116 a          922 c         1001 g          985 t
ORIGIN      
        1 aaaccattag agaggatatg ttgtaaatag ttccttctca tctctgtgat tttttgagag
       61 cccggtcaaa taatggagaa gtcagtattt ctccgatgtc atcgttgttc tgtaatgcat
      121 gcggacgctc acagttatat tcagacaacc atttctccgt aatttcccgt acttcattca
      181 gcgttctgaa cagataaaaa tagagtattt ctatacggta tgtccttgta gtggatcaca
      241 aaatcaggac acggggataa cctgtttcca ctatcttcat gtaagcaagg cggtaaacca
      301 tcgttgctcg tgtgaggtcg ataaacgttg taataaccat taatcctctg gtttatatcg
      361 cgtaccacat ggctaaaatc accataacca cctttcggaa gccatttact tttaaggctg
      421 caaaatactc tttccatcgg cgaattttcc aggcaattcc ctctgcgact catactttgc
      481 attatcccat aatgccatag taaatttctg tatttattgc ttttatactg aacaccttga
      541 tctgaataaa acggcaggcg gccatcacgc gggagtgttt ccagtgcatt acgcaaagcc
      601 ctacacacca atcagcatca gcggttaatg agagggctga accgataatc caccgtgaat
      661 ataaatcaac aacgagcgtg agataacacc atttgtccta caggcgaata aaactgatgt
      721 cgccgcacca gacgcggttt ggtgcagcgg gggggatgcg gacgatttcc tggactctca
      781 ggagataaaa catgtttact gaagaagaaa aaatccgcgc cattgagctt tatttcaaat
      841 atggtaagag gctggctccc gtcgtacgcg aactgggcta cccttcgaaa agaaatctgc
      901 ggcgttggat ccgttcatgg gaagcaggtg gtggcgcaaa agaatccatt cgtcataagc
      961 cccgttacac agatgagcaa aaacaggttg ccgttgaaca ttatcttaac cacggctgct
     1021 gcctggcgtt taccagcaga accctgggtt atccctgcac cgatgtgctc gctcgctggg
     1081 ttaatgaacg ttgccccgac agacggcgta tatttaccag taataccagt ccggttgcac
     1141 cctttgaacc agaagtcaaa cgtcaggctg tcatggcact ctgtacacga caaggatcag
     1201 ccagtgaaat cgccaggcgt attggtgtca gtcgtgcggt attgtataaa tggaaagatg
     1261 aaattatcgg caacagtgct taccagacta tgcgtaaaca taacgaccct tccctggagg
     1321 cagaacgcga tgtgttgcgg gaggaagtcg cccgactgaa tcaggaaata cgccgctggc
     1381 agatggagct ggatattctg aaaaaagcgg aggaaatcat aaaaaaagac ccgggcatca
     1441 gtatcagtca cctgaacaac agagaaaaaa caaagattac tgatgccctg agacaaacat
     1501 atcccctgac agaattactg catgttctgg gtcttgcccg tagcagttat ttttatcacc
     1561 gggctgcact gaaagccggt gataaatacg ccacgatacg tacgatgctg acagatatct
     1621 ttaacagtaa ttaccagtgt tatggctatc gtcgcctgca tgcgatgctc aggcatgagg
     1681 ggatacggct atcagaaaag gttgtacgca gacttatggt ggaggaacag cttgtcgtca
     1741 gccgtaaccg tcgtcgccgc tacagctcat attgtggaga gatcggtccg gctccggata
     1801 accttatcgc cagagatttt aaggcggagc aacctaatca gaaatggctg acagatatca
     1861 cggagtgata gcttcctgca gaaaaagtca ggctgtcacc ggtggtggac tgcttcgatg
     1921 gaaaagttgt gagcagttct ctctgtacac gtcccgacgc agaactggtt aacaccatgc
     1981 tggataacgc tgtcgaaacg ttaaatgctg gcgaacgacc ggtgatacac agtgacagag
     2041 gtgggcatta tcgctggcca ggctggctgg gaagagtgaa tgcagcaggc cttattcgct
     2101 caatgtcccg taaaggatgt tcgcctgata atgctgcatg cgaaggcttt ttcggcaggc
     2161 tgaaaactga aatgtattat ggacgtaaat ggtcaggcat cacgcctgaa aagttcatgc
     2221 agcatgtgga tgcttacatc agatggtata acgagcggcg tataaaatta tcgctgggtg
     2281 cagtcagccc tgaaatgtac cgctaacaat gcgggctggc ataataaagc agtccaggaa
     2341 atcgtccgca tccccataac ggtcaaacat cgtggcgttg acacgttgta tgccaaaggg
     2401 ataaccaccc gtgagatagc tgctacattc aaagaactgt atgacgcaga tgtttcaccg
     2461 gcactgatat caaaggtcac cgatgccgtg atggagcagg ttgtagaatg tcaaaacaaa
     2521 ccactggatg ctgtttaccc cattgtttat cttgactgta tcgtcctgaa agttcggcag
     2581 gacagtagcg tcatcaacaa atcggtgttc ctggcactgg gtatcaatat cgaaggtcag
     2641 aaagaactgc caggtatatg gctggccgaa aatgtcaacg ccagttgtag agctgtgatt
     2701 catacaggct gagttcacgg gctgcggcag caacaccgat gcgttcagca agtttcaggg
     2761 cttcactgcg aaattcaggc gaatgctgtt tacgtgcttt tttgctggct gatacagatt
     2821 ttgtcatgtg agtcacctct ggctgagagt ttactcactt agccgcgtgt ccactattgc
     2881 tggataagat caagtataca ggcacctatg gatcaggtct gcttctctat ttacagaatt
     2941 ccgggttaga agttcttgag gtgactgcgc cagatcggat ggagcgacgc aaacggggta
     3001 aaagtgacac gattgatgct gaatgtgccg ctcatgccgc attctccgga ataagaaccg
     3061 tcacacccaa aacgccaatg gcatgattga gtctctgcgg gtattaaaaa cttgccgaaa
     3121 aacagcaata tcagcccgca gagtcgctct ccagattatc catttcaata ttatctctgc
     3181 cccggatgaa ttacgtgaac agctcagaaa tatgacgcgc atgcagctta tcaggactct
     3241 gggctcctgg cggcctgatg ccagtgaata ccgcaatgtt accaacgttt atcgtatttc
     3301 attaaagtcc cttgcccgac gctatctcga gttacatgac gaaatcgctg atttggatgt
     3361 catgattgcg gcaattgtcg atgaactggc gcctgaactg attaaacgta atgctattgg
     3421 atacgaaagc gcttcgcgtt gctgatcacg gcaggagaca atccccaacg attaagatca
     3481 gaatcaggtt ttgcggcact gtatggtgtc agccctgttc ccgtatcttc aggaaaaatg
     3541 aatcgttatc gacttaaccg gggtggagat cgtgctgcaa atagtgcact tcacatcatt
     3601 gccatcggac gtttgcgaac tgacgataaa acgaaggaat atgtcgccag acgagtagcg
     3661 gaagggcata caaaaatgga agcaatacgc tgcctgaagc gctatatctc acgcgaagtt
     3721 tatacattac tgcgtaatca aaacgggcag atcaacagca tcccgataac ggcttgactc
     3781 ttagaagggc gtccagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg
     3841 ataccagatc aggcagagta tgagccggcg cggaaagtgt tgggataaca gcccaatgga
     3901 acgcttcttc aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag
     3961 cgaggcagct cacgccataa cggattatat cgttggatat tactgcgcac taagaccgca
     4021 cgaa
//
LOCUS       DAJOSY010000207         4012 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJOSY010000207 DAJOSY010000000
VERSION     DAJOSY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.207"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            110..718
                     /locus_tag="OHN27_004310"
     CDS             110..718
                     /locus_tag="OHN27_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCQ2288629.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            816..2207
                     /gene="selA"
                     /locus_tag="OHN27_004311"
     CDS             816..2207
                     /gene="selA"
                     /locus_tag="OHN27_004311"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCQ2288630.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2204..>4012
                     /gene="selB"
                     /locus_tag="OHN27_004312"
     CDS             2204..>4012
                     /gene="selB"
                     /locus_tag="OHN27_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCQ2288631.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDH"
BASE COUNT          885 a         1022 c         1204 g          901 t
ORIGIN      
        1 accatgataa atatctgatt tattgggaag taacaaacca tcaacatttt ctgccctttt
       61 tcccatcacc aggccatact gatatttttc ctcgcacaaa ggactcggca tgaaactcgt
      121 cggtagctac accagcccgt ttgtacgcaa actttctatt ctgttgttag aaaagggcat
      181 aactttcgaa tttattaatg aactgcccta taacgcggac aacggcgtgg cgcaatttaa
      241 cccgttagga aaagtgccgg tgctggtgac cgaagagggc gaatgctggt ttgattcgcc
      301 gatcatcgct gaatacattg aattaatgaa tgttgctccg gcgatgttgc cgcgcgatcc
      361 gctggagtcg ttgcgggtgc gcaaaattga ggcgctggcg gatggcatta tggatgccgg
      421 gctggtatcg gtgcgtgaac aggcgcgtcc ggcggcgcag cagtctgaag atgaattgtt
      481 acgccagcgg gagaaaatca accgtagtct ggatgtgctg gaaggatatc tggtcgatgg
      541 cacactcaaa accgatacgg tcaatctggc gactatcgcc attgcctgtg ccgtcggata
      601 tctcaatttc cgccgcgttg cgccaggttg gtgtgtcgac cgcccacact tagtcaaact
      661 ggtcgaaaac ctgtttagcc gcgaaagttt tgctcgcacc gaaccgccaa aggcttgatg
      721 cgcgatatgt cctcctgacc catctcacgt tacaatccgt ggttatgtta aacgcccttc
      781 tccgtgtgag agggccttga tcagccaggt ttcctatgac aaccgaaacg cgttccctct
      841 atagtcaact tccggctatt gatcgcttat tgcgcgatag ctccttcctt tctttgcgtg
      901 atacttatgg tcacacccgc gtggtggaat tgttgcgtca gatgctcgac gaagcgcgag
      961 aagtgattcg tggcagccag acgctgcctg cgtggtgtga aaactgggcg caagaagtcg
     1021 atgcccggtt gacgaaagaa gcgcagagcg cgctgcgtcc ggtgatcaac ctgacgggaa
     1081 ccgtgctgca taccaacctt gggcgagctt tacaggcgga agccgcggtg gaagccgttg
     1141 cgcaggctat gcgttcgcca gtgaccctcg agtacgatct ggacgacgcc ggacgcggac
     1201 atcgcgatcg ggcgctggcg cagctgctgt gccgtattac gggggcggaa gatgcctgta
     1261 tcgtcaataa caatgcggcg gcggtgttat tgatgttggc ggccactgcc agcggaaaag
     1321 aggtggtggt atctcgcggc gaactggtgg agattggcgg cgcgtttcgt attcccgatg
     1381 ttatgcgtca ggcaggctgc accctacacg aagtagggac caccaaccgc acgcacgcga
     1441 atgattatcg tcaggcggtg aatgaaaata ccgcactgtt gatgaaagta cataccagta
     1501 actacagcat tcaggggttc actaaagcga tagatgaagc ggaactggtg gcgctcggca
     1561 aagagctgga tgttcccgta gtgactgatt taggcagtgg ctcgctggtc gatcttagcc
     1621 agtacggttt gccgaaagag ccaatgccgc aggagttgat tgcggcgggc gtcagtctgg
     1681 tgagtttctc cggcgacaag ttgttaggcg ggccgcaggc aggaattatt gttggtaaaa
     1741 aagagatgat cgcccgcctg caaagccacc cgctgaagcg tgcattacgc gcggataaaa
     1801 tgaccctcgc ggcgctggaa gccacgttgc gtctttattt acaccctgaa gctctgagtg
     1861 aaaaattacc gaccctgcgc ctgcttaccc gcagcgcaga ggtcattcaa atccaggcac
     1921 aacgtttaca ggctcccctt gccgcacatt acggcgcgga gtttgcggta caggttatgc
     1981 catgtctttc gcagattggc agtggttcgc tgccggttga tcgcctgccg agcgcggcat
     2041 taacgtttac accccatgat ggacgcggta gccaccttga gtcattagcc gcccgctggc
     2101 gtgaattgcc agtgccggtg attggtcgta tttatgacgg acgattgtgg ctggatttac
     2161 gctgccttga agatgagcaa cggtttttgg agatgttgtt gaaatgatta ttgcgactgc
     2221 cggacacgtt gaccacggca aaacaacctt attgcaggcg attactggcg taaatgctga
     2281 ccgtctgccg gaagaaaaaa agcgcggcat gaccatcgat ctcggctatg cctactggcc
     2341 gcagccggat ggtcgcgtgc ctggttttat cgacgttccc ggtcatgaaa aatttctttc
     2401 caacatgctg gcgggcgttg gtggtatcga tcacgcgctg ttggtggtgg cgtgcgatga
     2461 cggtgtgatg gcacagaccc gtgagcatct ggcgattttg cagctgaccg gtaacccgat
     2521 gctgacagtg gtgctgacca aagccgatcg cgtggacgaa gcgcgtgttg atgaggttga
     2581 acgccaggta aaggaggtgc tgcgggaata cggttttgct gaggcaaaac tgtttatcac
     2641 cgcagcaacc gaaggtcggg gaattgatgc cctgcgcgag catctgcttc agttaccaga
     2701 acgtgaacac gccagccaac atagtttccg cctcgcgatc gaccgcgcat ttaccgtaaa
     2761 aggtgccggg ctggtcgtca ccggtacggc gttaagcggg gaagtgaagg taggcgattc
     2821 actatggctg actggtgtaa ataaaccgat gcgcgtgcgt gcgctgcatg cgcaaaacca
     2881 gccaacagaa accgctcatg ccgggcagcg tatcgcgctt aacatcgcgg gggatgcgga
     2941 aaaagagcag attaaccgtg gcgactggct gcttgccgat gtgccgccag agccgttcac
     3001 gcgggtgatt gtcgagcttc aaacccatac gccgctgacc cagtggcagc cgctgcatat
     3061 tcaccacgcc gccagccacg ttacgggacg cgtttcgctg ctggaagata accttgccga
     3121 gctggtgttc gacacgccgc tgtggctggc ggataacgat cgcttagtgt tgcgcgatat
     3181 ctcggcccgc aatacgctgg cgggggcgcg tgtggtgatg cttaacccgc cgcgtcgcgg
     3241 taaacgtaag ccggaatatc tgcaatggct ggcgtctctt gcacgggcgc agagcgatgc
     3301 cgatgcgcta tctgttcatc tggaacgcgg cgcggttaac cttgcggatt tcgcctgggc
     3361 gcgccagctc aacggcgaag ggatgcgcga attgctgcaa cagcctggtt atattcaggc
     3421 tggttatagc ttgttgaatg cgccggttgc cgcccgctgg cagcggaaaa ttctcgacac
     3481 attagcgact tatcatgagc aacatcgcga tgaacctggt cctgggcgcg aacgtctgcg
     3541 acgtatggcg ttgccaatgg aagatgaagc gctggtactg ttgctgattg aaaagatgcg
     3601 cgaaagcggc gacatcctca gtcatcacgg ttggctgcat ctgccagatc acaaagcggg
     3661 cttcagcgaa gagcagcagg ccatctggca aaaagcagag ccgctgtttg gtgacgagcc
     3721 gtggtgggtg cgtgacctgg caaaagagac gggaaccgac gagcaggcaa tgcgcctgac
     3781 gctgcgccag gcggcgcagc aaggaataat taccgcgatc gtcaaagatc gttattaccg
     3841 taacgatcgg attgtcgaat ttgccaatat gatccgcgat ctcgatcagg agtgtggttc
     3901 aacctgcgcg gcggatttcc gcgatcgctt aggcgtaggc cgaaagctgg caattcagat
     3961 tctggaatat tttgaccgca ttggctttac gcgtcgtcgt ggaaatgatc at
//
LOCUS       DAJOSY010000208         3914 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJOSY010000208 DAJOSY010000000
VERSION     DAJOSY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.208"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..243
                     /gene="trbG"
                     /locus_tag="OHN27_004313"
     CDS             1..243
                     /gene="trbG"
                     /locus_tag="OHN27_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HCQ2288632.1"
                     /translation="MVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVAYVY
                     KSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            240..755
                     /gene="traV"
                     /locus_tag="OHN27_004314"
     CDS             240..755
                     /gene="traV"
                     /locus_tag="OHN27_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HCQ2288633.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            890..1111
                     /gene="traR"
                     /locus_tag="OHN27_004315"
     CDS             890..1111
                     /gene="traR"
                     /locus_tag="OHN27_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HCQ2288634.1"
                     /translation="MSDEVDEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            1104..1580
                     /locus_tag="OHN27_004316"
     CDS             1104..1580
                     /locus_tag="OHN27_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288635.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQSLLQLMRLTEDVPDA
                     RQELLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEENKSE"
     gene            1660..1878
                     /locus_tag="OHN27_004317"
     CDS             1660..1878
                     /locus_tag="OHN27_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288636.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMETLNGGGQ"
     gene            1906..2268
                     /locus_tag="OHN27_004318"
     CDS             1906..2268
                     /locus_tag="OHN27_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288637.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMLICGLFYNDYLEQQPATPEQLTEI
                     TQDIPCAAEAFKEAIKSDTSDYQPEPLSLGKAKELASACRERNEMAEVKRVRENERNK
                     IREKQIQALNDAHSVKER"
     gene            2394..>3914
                     /locus_tag="OHN27_004319"
     CDS             2394..>3914
                     /locus_tag="OHN27_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HCQ2288638.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDS"
BASE COUNT         1043 a          895 c         1023 g          953 t
ORIGIN      
        1 ctggtttctg atggcagcgt caaaaaaata aattatccgg tgctgtatga gtccggaata
       61 acacctccgt tatgtgaagt cagtgcacca gaacctgatg cgggtgggaa acgcattgtg
      121 gcatatgttt ataaatcatc acggagtacg gtttttgaaa atcctgacat tgtaaaaacc
      181 tgtacggtca gggatttgaa gaaggatttt gtgaactgcg atgagaaagg tgagggacaa
      241 tgaaacagat ttctttcttt attcctctgc tggggacctt attactttcc ggctgtgccg
      301 gaaccagtac ggaatttgag tgtaacgcca ccacatccga tacctgtatg acgatggagc
      361 aggccaatga gaaggccaaa aaactggagc agtcctcaga agcaaagccg gttgcggcat
      421 cactgccgcg cctggctgaa gggaacttcc ggacaatgcc ggtgcagacc gtcaccgcga
      481 ccactccgtc cggcagcaga cctgcggtga cagcacatcc ggagcagaaa ctgctggcac
      541 ctcgcccgct gtttaccgct gcccgggaag tgaaaacggt tgttccggtc agttcagtta
      601 cgccggtaac acctccccgt ccgctaagaa cgggtgagca gacggcagca ttatggatag
      661 cgccttatat tgataaccag gatgtttatc atcagccatc cagcgtgttt tttgttatta
      721 aaccgtctgc gtggggaaaa ccacgtatta attaactggc cctgaagttg tatatcagcc
      781 ggcaatgccg gcgggctttc tgtattcaaa atctgcctgt aaaacgagtg ccctgtgcgt
      841 gaaaagggat gggcggtagc gtgtcagaac atgtcgggag gaatattccg tgagtgatga
      901 agtcgatgaa gcatattcag tgacagaaca actgaccatg acaggaataa accggatacg
      961 ccagaaaata aatgctcatg gtattcctgt ttatctctgt gaagcatgcg gaaatcctat
     1021 tccggaagcc cggcggaaaa tatttcccgg tgtgacgttg tgcgttgaat gtcaggcgta
     1081 tcaggaaagg cagaggaaac actatgcata aatcagaaaa tgaaaaaata gattttattc
     1141 tggatgaaca tgagcggata tccagaaaac agggttcttt gtgtatggcg ctgggtaagg
     1201 gctttttagt tatagctatt ctttgtggtg ttgccacatt ctgtgtttct ggcctgtatc
     1261 tgaaatccct gtgcctgtcc gttgcctgct gttgtggggt attcagtcgt atttttaatt
     1321 attacagcgt accggaggaa tcacgcatgg tcctcagccg tcagagcctg cttcagctta
     1381 tgcgtctgac tgaagacgta ccggatgcga gacaggaact gcttaaccgt ttgttgtccg
     1441 gtaaaaaact gactggtcgg gatgaacagg atatccggcg tctctggcag gagaaagtgg
     1501 atgcaatgca ggagtctgcc acccgtcagc gtgaacagga taccatccgg aagtttactg
     1561 aagagaataa atccgaatga gaatctgcag tgttttatga cctcataagt taactgaagg
     1621 gatattcagt gattaatatt taacaggcgg taataaagaa tgcatacctt tatttttctt
     1681 gtgctgataa aaaatattaa cggtggcgga agctggaatg ttactcccat gccaaatacg
     1741 gacgtatgcg tgcaggttct tcggacaatc gaagcacacg gaggcagggg ctttggaaat
     1801 acaggggaat gtattgaagt taaaacaaat gaacctttga acaacgtgat ggaaacactg
     1861 aatggtggtg ggcaatgagt gcttactgct tcgaaggaaa atgaaatgaa gtcgatatat
     1921 ttaaaatctg ttctggcttt tatatttgtt ggcgttatgg ctatgttgat atgtggtctt
     1981 ttttacaatg actaccttga gcaacaacct gcaacaccag aacaactgac agagattaca
     2041 caggatatcc cctgtgcagc agaagcattt aaggaagcta ttaagtctga cacatctgat
     2101 tatcagcctg aacctttaag tcttggtaag gcaaaagagc ttgcatcagc gtgtagagaa
     2161 agaaacgaaa tggcagaggt gaaacgggta agagaaaatg agcgcaataa gattcgcgaa
     2221 aaacaaattc aggcattaaa tgatgctcat tcagtaaagg aacgttaatc ctggtttcct
     2281 gttaaaagcc tccatctctt atttgtgctg tggtttcagg aatggcactg cgtgatatat
     2341 ccacggccac agactgatat tttccttttt taaacgcact aaggaatact catatgagta
     2401 ataacccact tgaggccgtc actcaggcgg ttaactccct cgtcacagca ctgaaactgc
     2461 ctgacgaatc agcaaaggcc aatgaagtgc tgggcgaaat gagcttcccg cagttcagcc
     2521 gtctgctgcc gtatcgtgat tacaaccagg aatccggtct gttcatgaat gacaccacga
     2581 tgggctttat gctggaagcc attcccatca atggggcgaa tgagtccatt gtggaggccc
     2641 tcgatcacat gctgcgcacc aaactgccgc gcggtattcc gttgtgtatc catctgatgt
     2701 ccagtcagct ggttggtgac aggattgaat acgggctgcg tgagttctcc tggtcaggtg
     2761 aacaggctga acggtttaac gccattaccc gggcctatta tatgaacgcg gcagcgacac
     2821 agtttccgct gccggagggg atgaatctgc ccctgaccct gcgccattac cgggtgttta
     2881 tctcgtactg ttctccttcg aagaaaaaaa gccgggccga cattctggaa atggaaaacc
     2941 tggtgaaaat catccgggcg tcgttacagg gggccagtat caccacacag acggtggatg
     3001 cacaggcctt tatcgatatt gtcggggaga tgattaacca taacccggac tccctgtacc
     3061 cgaaaagacg tcagctggac ccgtattctg atctgaatta tcagtgtgtg gaggacagtt
     3121 ttgacctgaa agtccgggct gattacctga cgctgggcct gcgtgagaac ggcaggaaca
     3181 gcacggcccg catcctgaat ttccatctgg cccgtaaccc ggaaatcgcc ttcctgtgga
     3241 acatggccga caactacagc aacctgctga acccggaact gtccatctcc tgtccgttca
     3301 tcctgacgct gactctggtg gtggaagacc aggtgaaaac ccacagcgaa gccaacctga
     3361 agtacatgga cctggagaaa aagtcgaaga cctcctatgc caaatggttt ccgtccgtgg
     3421 agaaagaggc gaaggagtgg ggggaactgc gtcagcggct gggctccggt cagtcctctg
     3481 tcgtgtccta cttcctcaac atcacagcct tctgcaagga caataatgaa acggcactgg
     3541 aagtggagca ggacatcctg aacagcttcc gtaaaaacgg ttttgagctg atttcaccgc
     3601 gctttaacca catgcgcaat ttcctgacct gtctgccctt tatggccggg aaagggttgt
     3661 ttaaacagct gaaagaggcc ggagtggtac agcgcgcaga gagctttaat gtggccaacc
     3721 tgatgccgtt agtggcggat aaccccctga caccggcagg tctgctggca cccacctacc
     3781 gtaaccagct ggcgtttatc gatattttct tccgggggat gaataacacc aactataaca
     3841 tggcggtctg tggcacctcc ggggccggta aaaccgggct gatacagcca cttatccgca
     3901 gcgtgctgga ctcc
//
LOCUS       DAJOSY010000209         3677 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJOSY010000209 DAJOSY010000000
VERSION     DAJOSY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.209"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(1..942)
                     /locus_tag="OHN27_004320"
                     /pseudo
     CDS             complement(1..942)
                     /locus_tag="OHN27_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            961..1122
                     /locus_tag="OHN27_004321"
                     /pseudo
     CDS             961..1122
                     /locus_tag="OHN27_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1202..1494
                     /locus_tag="OHN27_004322"
                     /pseudo
     CDS             1202..1494
                     /locus_tag="OHN27_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1534..2568)
                     /locus_tag="OHN27_004323"
     CDS             complement(1534..2568)
                     /locus_tag="OHN27_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HCQ2288639.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(2571..3503)
                     /locus_tag="OHN27_004324"
     CDS             complement(2571..3503)
                     /locus_tag="OHN27_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2288640.1"
                     /translation="MLATILAGMLSPMQSAVNGQLGHWLQDGNACAVISFASGLVVMF
                     FIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASASALGVATFQTA
                     LISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQWHSTSFILLAI
                     LPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAIRVALGHVTVQL
                     PDVWWMYLGGPLGLMSISLMAILVRGLGLLMLGVASTAGQLLGSVLIDVLIPSLGNTV
                     YLVTIIGTLFALVGAIVTTIPEYRTSKTMKKMEV"
     gene            complement(3534..>3677)
                     /locus_tag="OHN27_004325"
     CDS             complement(3534..>3677)
                     /locus_tag="OHN27_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCQ2288641.1"
                     /translation="YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIK
                     HYQ"
BASE COUNT          947 a          934 c          889 g          907 t
ORIGIN      
        1 gagacgacgg aagatcgttg ccgaagcggc gttccaccgc tcccagcatg acgtcctgta
       61 ctgtttcact gttgaagccg ccggtagtga ccgcccagtg cagtgcctca cgatcacagc
      121 agtccagcgc gaacgtgaca cgcagtctct ctccgttatc acagcagaac tcgaacccgt
      181 cagagcacca tcgctgattg ctttctttca cggccactct gcctgtatgt gcccgtttcg
      241 atggcggtac agcaggtttt cgctcaagca acagcgcatt ctggcgcatg atccggtaaa
      301 cacgtttggc attgatcgca ggcataccat caagttctgc ctgtctgcga agcagcgccc
      361 atacccgacg ataaccatac gtgggcagct ctccgataac atggtgtata cggagaagca
      421 catccgtatc atcagtgtga cgactgcggc ggccatccat ccagtcatcg gttcgtctga
      481 gaatgacgtg caactgcgca cgcgacaccc ggagacaacg gctgactaag cttactcccc
      541 atccccgggc aataagggcg cgtgctctat ccactttttt gcccgtccat attcaacggc
      601 ttctttgagg agttcatttt ccatcgtttt cttgccgagc aggcgctgga gttctttaat
      661 ctgcttcatg gcggcagcaa gttcagaggc aggaacaacc tgttctccgg cggcgacagc
      721 agtaagactt ccttcctggt attgcttacg ccagagaaat aactggctgg ctgctacacc
      781 atgttgccgg gcaacgaggg agaccgtcat ccccggttca aagctctgct gaacaattgc
      841 gatcttttcc tgtgtggtac gccgtctgcg tttctccggc cctaagacat caatcatctg
      901 ttctccaatg actagtctaa aaactagtat taagactatc acttatttaa gtgatactgg
      961 ttggaagctg atgctgcggc tatatgtgag gcaatcagca gtcgctggag taatggtgtt
     1021 gtcgaaggtc atgtaaatcg cctgaaggtg ctgatacgcc agatgtatgg tcgagccgga
     1081 tttgaactac ttaggcggag ggtaatgagt ccactggcat gaaaatcttc acctaggttg
     1141 cggaagagcc acttttgcac tgttcgtgac agtatggcct ttatgtactc actttgttac
     1201 cggtagctga atcatcacta ccgtgctgca aatggtggtg aacagcatca attgtggaat
     1261 gaatgttgcc gaagcgacca atgcgcctcg tttccaccat cagtggttgc cggacgagct
     1321 gcgtgttgaa aagggtttag tccggatacg ctcaagctgc tggaagcaaa aggtcagaaa
     1381 gtggcgctga atgaggcgat gggcagcacg caaagcatta tggttgggtc ggatggtgag
     1441 ttgtacggcg catccgactc gcgcttggtg gatgatttaa cggcggggta ttaattgaca
     1501 tcccccggcg cagtgtgccg ggggataatg gttttatgcg gtcaataatc gtccgggatt
     1561 atcgatacag agttttttga ggatcgtttt atcgacgccg cgctccgcca gataggccag
     1621 aaaaacatct ggaacaaatc cccagccatt accgccattt ttagcccaca tttgtttaag
     1681 gaagacatcg tggctgagaa caagctgatc ggcgtatccc aactcaatga gatgagcgac
     1741 ggcatcggca gtctcctgca cccctggcgc tattccctct ttcgggaagg taatgtctag
     1801 gccaatcatg tcgaattcca gccagacacc cttatcaagc attttccgct gatacgccac
     1861 gtcttttcct gacggatccg agtgtgcgag agagacttta tttggcgaga cgcccatttc
     1921 acctaacaca atgtcgagta cttcatcacc gcgacgaagc cagcccggca tgtgaatatt
     1981 catcgccaca tgaggattgt taatctgtgc cagcgaggca gcccgcaagc tgttatgctc
     2041 ggcttcggta aatgtcggtg agacacctat ttcaccgatc attccggcac gaatatccgt
     2101 atcgccaatc ccttgattca attctttatc aatcgttgcc gccagttcat caaccgtttt
     2161 atgaattctc tggctttcaa atttttccag gtagggaccg gaggaagcaa caatattcag
     2221 acctgttttt aatgcgactt ctcgtaatgc ctgcgcatct cgtccgatag actcagaccc
     2281 tgtggcatca acaatggtgc gaccaccgag cgagataaag ttattaattt cgaagatcac
     2341 atcttcaata ggtttgcggt ccatattatc agcgcagcaa taaggatcgt gttttaatgc
     2401 ccactggatt tctgccgtga cttttttatc aacaagccgt tgtgaaaaag gatagcaagg
     2461 agcatccaca acagaggaaa gatcgttgaa cagatgttca tgaggaagtg ttagccccat
     2521 atcttcacgg gcgacggggc ccgtcacagt ttgtagataa tctttcatga ttatacctcc
     2581 attttcttca ttgttttcga ggttctgtat tccggaatag tggtaacaat tgcaccgacc
     2641 agtgcaaaca gtgttccgat gatcgtgacc agataaacgg tattgcccag cgatggaatt
     2701 aatacgtcaa tcaatactga tcccagtaac tggcccgccg ttgaagcaac ccccagcatc
     2761 agtaacccca gacctctgac caaaatggcc attaaactga tagacattag tccaagcggg
     2821 ccgccaagat acatccacca gacatctggt aattgaacag tgacgtgccc taatgcgaca
     2881 cgaatagcta gcgctgtgcc taaaacacaa aatccaacga tgaaattcca ggtgattgaa
     2941 accagcatgg atcccgtagc ttcagctact tttgcatttc cagcgggctg ccatcctgca
     3001 agcaaaccag ctaagaacgg aagaatggca agtaagatga aagaggtaga atgccactgc
     3061 ggtgacacaa caaaaatcgt agcaataacc gcaaataatg caccgatgat gcgataaggg
     3121 gtaaagtatt ttttctcgtc gacaccgata ccaaagcgat cgcaaagtaa accagacagc
     3181 aatagcgcgg agatcagtgc tgtttgaaac gttgcgaccc cgagtgcgct ggctgatgcg
     3241 ccttcagaaa ataccaccat cgctccacag agaccggcaa accagttcca gagaggaact
     3301 tttcggtttt taatcagaga aggaattgcc gcaaattgtt gccgggtttc ttttcgcgcc
     3361 ataataataa aaaacatcac gactaaacca ctggcaaagg agataactgc gcatgcatta
     3421 ccatcctgta accagtgacc taattgacca ttcaccgctg attgcatagg ggagagcata
     3481 cccgcaagaa tcgtggcaag catccataag ggagttgagg taatgactcc aacttactga
     3541 tagtgtttta tgttcagata atgcccgatg accttgtcat gcagctccac cgattttgag
     3601 aacgacagtg acttccgtcc cagccttgcc agatgttgtc tcagattcag attatgtcgc
     3661 tcaatgcgct gcgtata
//
LOCUS       DAJOSY010000210         3554 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJOSY010000210 DAJOSY010000000
VERSION     DAJOSY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.210"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(100..534)
                     /locus_tag="OHN27_004326"
     CDS             complement(100..534)
                     /locus_tag="OHN27_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HCQ2288642.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNRHEND"
     gene            complement(548..769)
                     /locus_tag="OHN27_004327"
     CDS             complement(548..769)
                     /locus_tag="OHN27_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288643.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(770..1453)
                     /locus_tag="OHN27_004328"
     CDS             complement(770..1453)
                     /locus_tag="OHN27_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCQ2288644.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMFRVLKKDALMVSFYGWNRVDRFMVAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIENF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(1529..1841)
                     /locus_tag="OHN27_004329"
                     /pseudo
     CDS             complement(1529..1841)
                     /locus_tag="OHN27_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1838..2740)
                     /locus_tag="OHN27_004330"
     CDS             complement(1838..2740)
                     /locus_tag="OHN27_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCQ2288645.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVWFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            3179..>3554
                     /locus_tag="OHN27_004331"
     CDS             3179..>3554
                     /locus_tag="OHN27_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HCQ2288646.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKA"
BASE COUNT          859 a         1009 c          939 g          747 t
ORIGIN      
        1 ccagcagcag gttgcgggcg tgacgacaaa tcagtgcatt cagcgtttcg ttcatgattc
       61 atggtctccc tgaatcgccg cccgcaggcg gcgataacat cagtcgtttt cgtgtctgtt
      121 gtttttcagc agggtgcggg cctgattaat caccgcctga cggcgggtga cgcattccgg
      181 gacggcgagg ccatgatccc gggcggccca ttcacgtttc cacaatgcct gttcagcggt
      241 ctgaatcagg gacgccagca gttcagcagg gacggtcacc tggcggtttt cgctcacgtt
      301 attgatgatc tccatcgcca cgccggaaga aataccggat tcagtccgct ggtcttccgg
      361 gatgtcctcc aggcgcgcca ggacggttct gatttcatga tcggacaggg aaatatccat
      421 tccgtcagcg agggcctgaa tctcttccgg ggaccagata accagcatca gcggcatgtc
      481 tcccggatac tttactgcca gctcccggca tagcgtttcg catgttccgt acattctcgt
      541 ctccttctca atctttaaag cacgcttcca gttccagaat ttcggtatat gcctccatca
      601 ggaggcggtt tgcgcgaaac agcgtctgac gcaccagacg ttccggcagg gtgtcggcat
      661 taaagctgta ccgctgctcc atctcattca tggttgtacg caggtcgcac atggcattgg
      721 ccacctccgc cacttcggcg cgcagttttt cgtgtcctgc atagttcatt tacgcagcct
      781 ccggcataaa ccagtcatca ttcgcggccc cctgctgcat ggcccgttgc acggcggcaa
      841 ggcgttgctg cccggcacgg tgatactgct caagcagctc gataccgata taccggcgtc
      901 cggactggag ggcggcgacg caggttgagc cgctgcctgc aaacgggtcc agcacaattg
      961 cgttcgggtg tgtgaagttc tcaatcagcg gttgcaggct ggtaacgggc ttttccgtcg
     1021 ggtgatggcg attgcccgaa tatttccagc ccagcacgtc cggcagcggg ttttgtggca
     1081 gacgtggacg gccttttgcc aggatgtagg cgcattcgtg gcgatagccc acatatgcgg
     1141 ccttcgatgt gtagtttttg gtgaagacca ggtgaccaac aacgctgaat cccgcatttt
     1201 tccaggcgac cataaagcga tcgacgcggt tccagccgta gaagctcacc attaacgcgt
     1261 cttttttcag tacgcggaac atttcattac aggccggttg cagccactca tcggttttat
     1321 cgccggcgat ggtgcgcccg gaacggtcac ggaaaccgac gagatatggc gggtcggtga
     1381 ggatgaaatc aatggcgtta tcagggatac gggccataac atcgatgcag ttaccgagga
     1441 caaaacggga cataccaaac tccttctctt acagagggca ccgggagggc acccctgtgg
     1501 tgcccgttcc ccgctgcctg tgggctgttc accgcgtccg gtaacgggtc aggccgaccg
     1561 tggaataccg cagccgtcgt ctttctgacg gcaaggttat gccgcgaaag cggcttgaag
     1621 ggcgttcatc agaaaagggc gacaggctgg cgtacactgt caccgacgcc agccgggagc
     1681 ggtaaaagcg cccgtcccgt gcccggccgg acgcgggaca ctgaccacag gcagcgttta
     1741 tgccgcgccg cgtcagccgg cgcagtaaaa agaggagcac ggcgacatcc tttaccggaa
     1801 aagaaacggg cggtagcccg cccgtctccg tcatacttca tccgccataa tgcgccacct
     1861 tgtcgactat ccacgcaggt ccggtgactt ccggcagctc atcgtcatct gtcggggaag
     1921 accattccag ctccccccac agcacatcaa cgagctctcc gcgctcatac aactgccagc
     1981 cacagaaatc acagccctgt tcggcatacc agtgctccag cgtgcagctg aaacggcggc
     2041 ttaattctgc aataacgtcg ctttccggct gacaccacgg cgtgtcgaaa tcaacctgaa
     2101 tgaagttgtc tccctgactg ctgatattca ggtcataccc acaaggccac ttcacgccat
     2161 actgttcggt ataccagtga tatgcagaag gaacaccgtt cagaacacca ccgttaaagc
     2221 cattcacttc aacatcaagg cgggtgggca gaaccgtcag caggtcaaac ggcatggttt
     2281 tttccggcag tacgttgtca cacagacggt tccaccacac cgatacatcc tcgttactcc
     2341 agaagccaca ccagtcacct ctttttgcgg tgaaaagcgc ggtgatggta tccctgacat
     2401 cgtccggtaa tccttcccag cgacgctggc cagtaccgct ctgcagccag agttcatgca
     2461 gcatcaggca gttttgctca tccagcagta caccgttctg caggtgtgtc agccagcggg
     2521 tgaacgcgat attctccggc gatacaacgc cacgtccggc agccgtcagt ccggggcacg
     2581 gttcaaacca cacatcttca gtggtctgca gaagtccggc acttccggcc agaaacagct
     2641 gaataccttc atttgtggcg cggcgataaa acggtgtgac ggcaccactg gccagtcgtt
     2701 taatctcagc aatctgtgcc ggttcaccag aaaaatgcat acggttactg caccagtttg
     2761 gcatgggaat tctcctctca catgcggcct ccatcaggcc gtgccgcacc tgtaaccggg
     2821 cgaccgcata cgaatgcagg tgcggccccg aagccgcagc gcgtggagga ggaacgacga
     2881 aacgtgaccg ggtgaggagg ccggcgacag cgacacactt gcatcggatg cagcccggtt
     2941 aacgtgccgg cacggcctgg ttaagtagtt ttcgtctcag tttgtgacgc aaaatgagaa
     3001 accaataaca acccaaattg agtcgcagca agaacacaag gagactctaa aagagccacg
     3061 ttacttgaca ttaacccaaa atgagacaca ataagaaata ataaaaactc aaaaagatgc
     3121 atcgacataa aagatgctca atttgagaca aacacaaact caaaacgagg caaaaagaat
     3181 gctaaaagta tcatgcgatg atggttcaac taacgtaaaa ctagcctggc ttgaagatgg
     3241 cgaggtaaga accagtctgt caggaaacag ttttaaagaa ggctggaacc cgggactgtt
     3301 taatgccggc aaagtttaca attacgtcgt ggatggaaaa aaatacacct atgatttggg
     3361 cagcactgct gtcataggta caacacatgt cagttatcaa tacagcacca ccaatctgct
     3421 tgccatacat catgcactgc tgacatccgg ccttcagcca caggatgtag aactgacagt
     3481 gacattacct gtaaccgaat tctttgataa tgacaatcaa ccaaatgaag aaagaataga
     3541 aagaaaaaag gcaa
//
LOCUS       DAJOSY010000211         3529 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJOSY010000211 DAJOSY010000000
VERSION     DAJOSY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.211"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            82..255
                     /locus_tag="OHN27_004332"
     CDS             82..255
                     /locus_tag="OHN27_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288647.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSKRSLVKQILIED
                     EIIVCATYLIPDI"
     gene            257..601
                     /locus_tag="OHN27_004333"
     CDS             257..601
                     /locus_tag="OHN27_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCQ2288648.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            605..940
                     /locus_tag="OHN27_004334"
     CDS             605..940
                     /locus_tag="OHN27_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCQ2288649.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(997..3239)
                     /locus_tag="OHN27_004335"
                     /pseudo
     CDS             complement(997..3239)
                     /locus_tag="OHN27_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          946 a          815 c          723 g         1045 t
ORIGIN      
        1 attgataaat cagattatct tacctttaca acattgcgtt aacagtcaat gacacttctt
       61 gaataaaaat attgtcaatc aatgagttat tcctcattta agattattct cattcacgta
      121 aaaaacataa ttcctatcat cacagccacg ctcattttga attatctgaa taatagcaag
      181 cgttctttag ttaagcagat cttaattgaa gacgaaatta ttgtgtgtgc aacttactta
      241 ataccggaca tctaagttga agaaaaaaat acttgccttc gggcttatct cagcattgtt
      301 ctgctccacg ccagcgatgg cggatatgaa ccgcaccacg aaaggtgcat tgctaggcgc
      361 aggcgttggt ttacttactg gcaatggtgt taatggcgta ctgaaaggtg cagctgttgg
      421 cgctggtgtt ggtgcagtaa cagaaaaagg ccgcgacggt aaaaatgcgc gtaaaggtgc
      481 aaaagtgggt gccgccgttg gtgctgtcac tggcgttctg accggcaatg gcctcgaagg
      541 ggcgattaaa ggtgccgtga ttggcggtac gggtggtgca attctgggta aaatgaaata
      601 aggaatgatg atgaaaaaat ttgctttact ggcgggcctg tttgttttcg caccaatgac
      661 ctgggcgcag gactacaata ttaaaaacgg tttgccgtca gaaacctata ttacttgcgc
      721 agaagccaac gaaatggcaa aaactgacag cgcacaggtt gcagaaattg ttgcggtaat
      781 gggtaatgcc agcgttgcca gccgtgattt aaaaattgag caatctccgg aactgagcgc
      841 caaagtggta gaaaaactga atcaggtttg cgctaaagac ccgcaaatgc tgttgatcac
      901 ggcaattgat gacaccatgc gtgctatcgg caaaaaataa tatttcatat gtaccgcgtc
      961 gatgaatacg acgcggtttt ttagctaacg gcgggatcag aacttatatt tcaggcccac
     1021 catcactgcg gtgtcattgt agccattatc acccagctgc acgcccacat tccccagacg
     1081 ctggccgctg gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcgcc
     1141 ccatcttgtt cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc
     1201 cagttcattt caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga
     1261 gtacgaataa aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca
     1321 atacgtgagc cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt
     1381 gcctgtggct ggaagatcca cgtatatttc gacgcttcca gaccaaagga tttattcagg
     1441 gcaaaaccgt aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag
     1501 gctaattcat cccctttcac cgagttttta aaccagctat attgcgccca ggagtcaatg
     1561 tatgcgcctt ttttactgat gtcatcggca aaccaggtgg catacagccc tgcgctatag
     1621 cctctgacac tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg
     1681 cgggcataac cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt
     1741 aacagatcgc cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg
     1801 ctgtcacgcc aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc
     1861 tgttcagtta caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca
     1921 aaaagagtgt tagcagctgc caggttagcg acgtaacttc cagcttcggg gcgcaggacg
     1981 ggggtaggcg tttctgttga ttctgaattt ccctctgagc tgttatcctc ctcattcact
     2041 gactgataac ttgtcaaata ccagtttttg ccttgttgtt ttagttgata ttcgaaagct
     2101 ccagctgcaa tccttgcatc accttatctg aaggcatctg ctggagattc atcgccattc
     2161 acagttatga gttcgatccc gtttagcgtg gccgcgccgc ttcctccagc atttttcaca
     2221 tagacggtag ttgaacccga agtattaccc tcaacaataa gtctgtccgt agccgattta
     2281 tcatcaccta atacggtgcg gatattaagc tgaccattat taccgacata attacctttt
     2341 acggttaatg tttttccttg ggtaaatgtt tgaccatcat catacgaaag attgataata
     2401 ctgtctgaat tagtcagatg ggtaacaacg gagtcatctt tcatattcca gattgtattt
     2461 tctgaaagct tcagcgtggt tgtgccgctg acgagtttag tcgctccatt tagaatagag
     2521 tcggacgaaa ttatgtttaa gtcacctcca gtattattaa ccagaacatt attaccaggt
     2581 gtgataatac ttcgattaag gatacttaca tccgttgtag ttttgttaac atcaatacta
     2641 tccaaagcac ttgaaagttc aacacttaat agtgctattt tggcttgaga atcgatattt
     2701 gatgcatcaa ttgatattcc tttatcttgt tttgataccg cggtgacatt ggaaaaaaat
     2761 aactctccac cattgtatat ttttgcaatg ggggctttag tgcctgttgt tgtaatagaa
     2821 ctatttttta tatcaatgtt accttgtcga atagcaactg cataacttcc atcagccaca
     2881 gtttcaagtg ccacataatc taaattaata tatgcttttt tcccattagc ataagcacca
     2941 tagctattaa tgccattagt tgttatggaa ccaccattca tttttaaatc agcacggcta
     3001 ataacttcgt aactgttaga atcactaact tcatcatcta cagcatcgac acatgttcga
     3061 gactctccag gacttgaaca tgcaagaaca cctactgcat tttccccaga ggtaacaatg
     3121 ttcgttttat ctgtggtttc aagacgggtg ttatttacca tcttttcaga atcattaacc
     3181 tggcttaaaa gtcccgctga atatgcccca cccgactcga ctgaattatc accattcata
     3241 aagatggtgc caccaaattc actgactact gcgctggctg ttatccctcc attttctttg
     3301 tcagatacgc ttccagaggt cttaatatcg ctatcgctta tatataatac tttgttttga
     3361 ctatcaacag aaataccata agatccatca ccactagtca tcacactggc attatttata
     3421 tatccagtta caccgtcatc cactttcaca gcatggcttt cgtcttcagt ggttagatac
     3481 tctccattac ttatcggatt attatctttg atttcaatcg gcgattctt
//
LOCUS       DAJOSY010000212         3393 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJOSY010000212 DAJOSY010000000
VERSION     DAJOSY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.212"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1100)
                     /locus_tag="OHN27_004336"
     CDS             complement(<1..1100)
                     /locus_tag="OHN27_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCQ2288650.1"
                     /translation="MKKKLVRFSALALAIGFLSGCTIIPGQGLNSLRKNVVELPDSDY
                     DLDKLVNVYPMTPGLIDQLRPEIILARPNPQLDNLLRSYEYRIGVGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVQVAGKTLSQVRQDIASRLTTYIESPQ
                     VDVSVAAFRSQKVYVTGEVSKSGQQPITNIPLTVMDAINAAGGLAPDADWRNVVLTHN
                     GKDTKISLYALMQKGDLTQNHMLYPGDILFVPRNDDLKVFVMGEVGKQSTMKMDRSGM
                     TLAEALGNAEGISQDMSDATGVFVIRQLKNDKSGKIANIYQLNAQDASAMVLGTEFQL
                     QPYDIVYVTTAPIVRWNRVISQLVPTISGVHD"
     gene            complement(1245..2678)
                     /locus_tag="OHN27_004337"
     CDS             complement(1245..2678)
                     /locus_tag="OHN27_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HCQ2288651.1"
                     /translation="MIKIARIAVTLGLLSSLGAQAYAAGLVVNDNDLRNDLAWLSDRG
                     VIHLSLSTWPLSQEEISRALKKAKPSYSSEQVVLARINQRLSALKADFRVTGYTSTDQ
                     PGTPQGFGQTQPADNSLGLAFNNSGEWWDVHLQGNVEGGERISNGSRFNANGAYGAVK
                     FWNQWLSFGQVPQWWGPGYEGSLIRGDAMRPMTGFLMQRAEQAAPETWWLRWVGPWQY
                     QISASQMNQYTAVPHAKIIGGRFTFTPFQSLELGASRIMQWGGEGRPQSFSSFWDGFT
                     GHDNTGTDNEPGNQLAGFDFKFKLEPTLGWPVSFYGQMVGEDESGYLPSANMFLGGVE
                     GHHGWGKDAVNWYVEAHDTRTNMSRTNYSYTHHIYKDGYYQQGYPLGDAMGGDGQLIA
                     GKVELITEDNQRWSTRLVYAKVNPENQSINKAFPHADTLKGLQLGWSGDVYQSVRLNT
                     SLWYTNANNSDSDDVGASAGIEIPFSL"
BASE COUNT          869 a          915 c          740 g          869 t
ORIGIN      
        1 atatcatgaa cgccagatat agtcggaact aactgagata tcactctgtt ccaacgaact
       61 attggagcag tggtaacata tactatgtca taaggctgta attgaaactc cgttccaagt
      121 accattgctg aggcatcttg agcatttaac tgatagatat tagcaatctt accactctta
      181 tcatttttta actgtcgtat aacaaatacg ccagttgcat cgctcatatc ctgtgaaata
      241 ccttcagcat tacctaaagc ttcggccaag gtcataccac ttcgatccat tttcatggtt
      301 gattgtttac caacctcccc catcacgaaa actttaagat catcatttcg tggtacaaat
      361 aaaatatcac ctgggtaaag catatgattt tgggtaagat cacctttttg catcagcgca
      421 taaagagaaa ttttggtatc ttttccatta tgcgttaaca ctacatttcg ccaatccgca
      481 tcgggagcca atcctcccgc agcattgatt gcatccataa ccgttaaagg tatattcgta
      541 atcggttgtt gacctgattt cgaaacttcg cctgtcacat atactttttg agatctaaat
      601 gcagccacgc taacatccac ttgcggactt tcaatataag tagtcaaacg gcttgctatg
      661 tcctggcgaa cctggctcaa agttttgccg gccacctgca ctttaccgat ataaggatag
      721 aaaatagtgc catctgaatt aacccagtta ccggtatcac ttgcgctacg gtattggcct
      781 gcaggtgtag tcaactccgg gtgatcccaa acagtgacca tcagtacatc gcctacaccg
      841 atgcgatatt cgtaactgcg cagtaaatta tcaagctgtg ggtttggacg agcgagtata
      901 atctccggac gaagctgatc gatcagtcct ggagtcatcg gataaacatt gaccaattta
      961 tccagatcgt agtcactgtc cggaagctca actacattct tacgcagact gtttaatccc
     1021 tgacccggaa taatggtaca acccgataaa aacccaattg ccaacgctaa tgccgaaaat
     1081 ctaacaagtt ttttcttcat aatgtcacat catcagtaaa tcaaaatttg ccaattcctg
     1141 agcagctgtc attgccgttg cttccctgcc ggcttgtgga gagataaagc tgactgcctc
     1201 ctgcagcgct accaccctct ctcccctatt ttcagcattc agccttataa actaaacggt
     1261 atctctatcc ctgcgctggc cccaacgtcg tcgctgtcgc tgttgttcgc gttggtgtac
     1321 cacaatgaag tattcaagcg gaccgactga taaacatctc cgctccagcc aagctgtaaa
     1381 cccttgaggg tatcagcatg agggaacgct ttattaatag actggttctc cgggttaact
     1441 ttggcgtaca ccaggcgcgt gctccagcgt tggttatctt cggtaataag ctcaacttta
     1501 ccagcaataa gttgaccatc tccgcccatc gcatcgccca gtggataccc ttgttggtaa
     1561 taaccatctt tgtagatgtg gtgggtatag ctgtaattgg tccggctcat attggtacgt
     1621 gtatcatgcg cttccacata ccagttaacc gcatccttac cccagccgtg gtgtccttca
     1681 accccgccaa gaaacatatt cgcagatggt aggtaaccag actcatcctc accaaccatc
     1741 tgcccataga aactcactgg ccagccaagg gttggttcaa gtttgaactt aaagtcaaac
     1801 cctgccagct ggttacccgg ttcgttatct gtcccggtat tatcatggcc agtgaagcca
     1861 tcccagaaac tgctgaatga ttgcggtcga ccttccccgc cccactgcat aatgcgcgac
     1921 gcgcctaatt ctaaagactg gaacggcgtg aaggtgaatc gaccaccaat aattttagca
     1981 tggggcacag cggtgtactg attcatctgg ctggcagaga tctggtactg ccatggaccc
     2041 acccagcgca gccaccaggt ttccggcgcc gcctgctcgg ctcgctgcat caggaaaccg
     2101 gtcatcggtc gcatcgcatc cccgcggatc aggcttcctt catagcccgg tccccaccac
     2161 tgcggaacct gaccaaaaga gagccactgg ttccagaact tcaccgcgcc gtaggcgccg
     2221 ttggcgttga agcgcgatcc gttgctgatc cgctcccccc cttcgacgtt accctgaagg
     2281 tggacatccc accactcgcc gctgttgtta aacgccaggc ctaaggagtt atccgccggc
     2341 tgcgtctgac caaacccctg cggggtgccc ggctggtcgg tcgaggtgta gccggtgacc
     2401 cggaaatcgg cttttaaagc agacagtcgc tggttgatac gggccagcac cacttgctca
     2461 gaagaatagg acggtttggc cttttttagc gcccgggaga tctcttcctg gctcagcggc
     2521 caagtcgaca ggctcagatg gatgacccca cgatcggaaa gccaggccag gtcgttacgc
     2581 aagtcgttgt catttactac taacccggcc gcgtaagcct gggctcccag ggaggaaagc
     2641 aaacccaacg tcacggcaat gcgcgcaatt tttatcattg tacaagatcc attttcagcc
     2701 ccgctgtcgc atctcaatga gaatgagggg aataccaaga tagaaagcgc tcgcggcacc
     2761 agctgacttc actgtgcaac accggcgagg caagtacagt taccctaagc tgcttgaaac
     2821 ccttataaca tccacccagc tcacaatgta ggcgaatttt accgtacagg ctatcgatga
     2881 gatgggaaag ttgatgtaca ggcgatatca gcagccgctc ctgacccctt tatgaggata
     2941 tctgatgagc atcatagata tcttatggcg ttaatcaccc aaagtgcatc gatttttata
     3001 gccgttcaga gaaggtattt tgtgcagccg cacgtggttt cagcgacgct accgcccctg
     3061 gcttaacagc taccaacgca ctgactacct gaacggcgta tttaatcggc ctgtaggtga
     3121 taaattggtc aggcctttat tttttgcggc gttaagtcta cgggatttcg gtcacaattg
     3181 ggagtcttgc tcccgaatat ctgccaatta atcccatccg ctgtgttatt ggcgaaaaat
     3241 aagcggctcg ccaataaaag attatttatt gttttcaact atcgaacatc tgcctggcgc
     3301 taccacaaca gcatagatac agctaaccat ttgaaaaaac taaaatttct cgcaactctg
     3361 acgtcattcc aaaatcagat gatgacgcca tag
//
LOCUS       DAJOSY010000213         3362 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJOSY010000213 DAJOSY010000000
VERSION     DAJOSY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.213"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            75..1040
                     /gene="dusB"
                     /locus_tag="OHN27_004338"
     CDS             75..1040
                     /gene="dusB"
                     /locus_tag="OHN27_004338"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCQ2288652.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1066..1362
                     /gene="fis"
                     /locus_tag="OHN27_004339"
     CDS             1066..1362
                     /gene="fis"
                     /locus_tag="OHN27_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCQ2288653.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1448..2332
                     /gene="yhdJ"
                     /locus_tag="OHN27_004340"
     CDS             1448..2332
                     /gene="yhdJ"
                     /locus_tag="OHN27_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCQ2288654.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NRSKRCRVSEIDPDLIAK"
     gene            2416..2595
                     /locus_tag="OHN27_004341"
     CDS             2416..2595
                     /locus_tag="OHN27_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCQ2288655.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2598..3260)
                     /gene="acrS"
                     /locus_tag="OHN27_004342"
     CDS             complement(2598..3260)
                     /gene="acrS"
                     /locus_tag="OHN27_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCQ2288656.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          920 a          775 c          804 g          863 t
ORIGIN      
        1 ttggcctttc atctcgtgca aaaaatgcgt aatatacgcc gccttgcagt cacagtatgg
       61 tcatttctta actcatgcgc atcggacaat atcagctcag aaatcgcctg atcgcagcgc
      121 ccatggctgg cattacagac agaccttttc ggacgttgtg ctacgagatg ggagccggat
      181 tgacagtatc cgagatgatg tcttctaacc cacaggtttg ggaaagcgac aaatctcgtt
      241 tacggatggt gcacattgat gaacccggta ttcgcaccgt gcaaattgct ggtagcgatc
      301 cgaaagaaat ggcagatgca gcacgtatta acgtggaaag cggtgcccag attattgata
      361 tcaatatggg gtgcccggct aaaaaagtga atcgcaagct cgcaggttca gccctcttgc
      421 agtacccgga tgtcgttaaa tcgatcctta ccgaggtcgt caatgcagtg gacgttcctg
      481 ttaccctgaa gattcgcacc ggctgggcac cggaacaccg taactgcgaa gagattgccc
      541 aactggctga agactgtggc attcaggctc tgaccattca tggccgtaca cgcgcctgtt
      601 tgttcaatgg agaagctgag tacgacagta ttcgggcagt taagcagaaa gtttccattc
      661 cggttatcgc gaatggcgac attactgacc cgcttaaagc cagagctgtg ctcgactata
      721 caggggcgga tgccctgatg ataggccgcg cagctcaggg aagaccctgg atctttcggg
      781 aaatccagca ttatctggac actggggagt tgctgccccc gctgcctttg gcagaggtta
      841 agcgcttgct ttgcgcgcac gttcgggaac tgcatgactt ttatggtccg gcaaaagggt
      901 accgaattgc acgtaaacac gtttcctggt atctccagga acacgctcca aatgaccagt
      961 ttcggcgcac attcaacgcc attgaggatg ccagcgaaca gctggaggcg ttggaggcat
     1021 acttcgaaaa ttttgcgtaa acagaaataa agagctgaca gaactatgtt cgaacaacgc
     1081 gtaaattctg acgtactgac cgtttctacc gttaactctc aggatcaggt aacccaaaaa
     1141 cccctgcgtg actcggttaa acaggcactg aagaactatt ttgctcaact gaatggtcag
     1201 gatgtgaatg acctctatga gctggtactg gctgaagtag aacagcccct gttggacatg
     1261 gtgatgcaat acacccgtgg taaccagacc cgtgctgcgc tgatgatggg catcaaccgt
     1321 ggtacgctgc gtaaaaaatt gaaaaaatac ggcatgaact aattcaggtt agctaaatgc
     1381 ttgattaaaa aggcgctact cggcatgggg aagcgccttt tttataggtg tcacaaaggg
     1441 agtgaccatg agaacaggat gtgaaccgac ccggtttggt aatgaagcta agaccattat
     1501 tcacggtgat gcccttgccg aacttaaaaa gctacctact gaaagcgtcg atctgatctt
     1561 tgccgaccca ccgtataaca tcggtaaaaa ttttgatggt ctgatcgaag cctggaaaga
     1621 agatctgttt atcgactggc tgtttgaagt gattgcagag tgccaccgcg ttctgaaaaa
     1681 gcagggcagc atgtacatta tgaacagtac ggaaaacatg ccctttatcg atctccagtg
     1741 ccgcaagctt tttaccatca aaagtcgcat cgtctggtca tatgacagtt ctggagtaca
     1801 ggcgaaaaaa cactacggct ccatgtacga acccatcctg atgatggtga aagacgcaaa
     1861 gaactacaca ttcaacggtg atgctattct ggtagaagcc aaaaccggat cgcagcgcgc
     1921 gttgatcgat tatcgcaaaa atcctccaca gccatacaat catcaaaaag taccgggtaa
     1981 cgtctgggat tttccgcgcg tgcgttattt aatggatgaa tatgagaacc acccgacgca
     2041 aaaaccggaa gccttactga aacgcattat tctcgcctct tccaacccag gcgatatcgt
     2101 tctcgacccg tttgccggta gctttactac cggtgccgta gccatcgcca gcggacgaaa
     2161 attcattggt attgagatca acagcgagta catcaaaatg gggcttcgac ggctggatgt
     2221 cgcgtcgcat tactccgcgg aagaactggc gaaagtgaaa aaaagaaaga cgggcaaccg
     2281 gtcaaaacga tgccgggtta gcgaaattga ccccgatctc attgcaaagt aaaggaagtg
     2341 taagcctggt tttcagatta ttcatttcgt gtatatttcc tgccagactt ggttaaacat
     2401 gcacaggctc aggtaatgat tcgcaagtat tggtggctcg tcgttttcgc tgtcttcgtt
     2461 tttctgtttg atactttact gatgcagtgg attgaactgc tggcaacaga aacagacaaa
     2521 tgccgtaata tgaactcagt taatccacta aaactggtta actgtgacga actgaatttt
     2581 caggacagaa tgtgaattta catgacactt aattcattcg tttgatgaat taatttcgtt
     2641 atgttttcat ctggcatgaa cattcttaat acgttatcga ccagcgcggg tgcttgttta
     2701 taaagatcat aacccgccat attcattaac cagttttgaa caattccgct gaaggcacca
     2761 tcaataataa tcatcacaac atctaaatcg aggttatttg ctacacaacc ttgttgctga
     2821 cacgcctgca atacttcgcg gagagtctgc ggattaaagc ccatcttttc gcgtatcact
     2881 ccctcggcca gcatctcatc attaaattca catttgtgat ataagatttt cagcaacgcc
     2941 tgctggcggg gaattttggc aatatattgc aagccgacaa tcaatttttc acgcaattgt
     3001 tgaaacgggt catgctctaa tccagccgtc aagtgttcct ggattaactc ccgcaatgaa
     3061 ggctgttgca accacatctc attaaacagt tgggtcttgt tctcgaagtg ccagtagata
     3121 gcgccacgcg taacgttagc ggcatcggca atgtcgttga gcgtcgtctt gcttacgcca
     3181 tgttgcgcaa actgggcgat ggcagtttca atcagttctt gccgggtctt cagagcttcg
     3241 gctttggttc tttttgccat gattaattat tcaggaaata aatatattcg acacagggtg
     3301 agaaaatagc gaaggttaat ctatcatcta atgtgtattt atacgagagg gtaacattga
     3361 gt
//
LOCUS       DAJOSY010000214         3351 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJOSY010000214 DAJOSY010000000
VERSION     DAJOSY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.214"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            60..1661
                     /gene="aceB"
                     /locus_tag="OHN27_004343"
     CDS             60..1661
                     /gene="aceB"
                     /locus_tag="OHN27_004343"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCQ2288657.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            1691..2995
                     /gene="aceA"
                     /locus_tag="OHN27_004344"
     CDS             1691..2995
                     /gene="aceA"
                     /locus_tag="OHN27_004344"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCQ2288658.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
BASE COUNT          820 a          877 c          924 g          730 t
ORIGIN      
        1 cacaacgatc cttcgttcac agtggggaag ttttcggatc catgacgagg agctgcacga
       61 tgactgaaca ggcaacaaca accgatgaac tggctttcac aaggccgtat ggcgagcagg
      121 agaagcaaat tcttactgcc gaagcggtag aatttctgac tgagctggtg acgcatttta
      181 cgccacaacg caataaactt ctggcagcgc gcattcagca gcagcaagat attgataacg
      241 gaacgttgcc tgattttatt tcggaaacag cttccattcg cgatgctgat tggaaaattc
      301 gcgggattcc tgcggactta gaagaccgcc gcgtagagat aactggcccg gtagagcgca
      361 agatggtgat caacgcgctc aacgccaatg tgaaagtctt tatggccgat ttcgaagatt
      421 cactggcacc agactggaac aaagtgatcg acgggcaaat taacctgcgt gatgcggtta
      481 acggcaccat cagttacact aatgaagcag gcaaaattta ccagctcaag cccaatccag
      541 cggttttgat ttgtcgggta cgcggtctgc acttgccgga aaaacatgtc acctggcgtg
      601 gtgaggcaat ccccggcagc ctgtttgatt ttgcgctcta tttcttccac aactatcagg
      661 cactgttggc aaagggcagt ggtccctatt tctatctgcc gaaaacccag tcctggcagg
      721 aagcggcctg gtggagcgaa gtcttcagct atgcagaaga tcgctttaat ctgccgcgcg
      781 gcaccatcaa ggcgacgttg ctgattgaaa cgctgcccgc cgtgttccag atggatgaaa
      841 tccttcacgc gctgcgtgac catattgttg gtctgaactg cggtcgttgg gattacatct
      901 tcagctatat caaaacgttg aaaaactatc ccgatcgcgt cctgccagac agacaggcag
      961 tgacgatgga taaaccattc ctgaatgctt actcacgcct gttgattaaa acctgccata
     1021 aacgcggtgc ttttgcgatg ggcggcatgg cggcgtttat tccgagcaaa gatgaagagc
     1081 acaataacca ggtgctcaac aaagtaaaag cggataaatc gctggaagcc aataacggtc
     1141 acgatggcac atggatcgct cacccaggcc ttgcggacac ggcaatggcg gtattcaacg
     1201 acattctcgg ctcccgtaaa aatcagcttg aagtgatgcg cgaacaagac gcgccgatta
     1261 ctgccgatca gctgctggca ccttgtgatg gtgaacgcac cgaagaaggt atgcgcgcca
     1321 acattcgcgt ggctgtgcag tacatcgaag cgtggatctc tggcaacggc tgtgtgccga
     1381 tttatggcct gatggaagat gcggcgacgg ctgaaatttc ccgtacctcg atctggcagt
     1441 ggatccatca tcaaaaaacg ttgagcaatg gcaaaccggt gaccaaagcc ttgttccgcc
     1501 agatgctggg cgaagagatg aaagtcattg ccagcgaact gggcgaagaa cgtttctccc
     1561 aggggcgttt tgacgatgcc gcacgcttga tggaacagat caccacttcc gatgagttaa
     1621 ttgatttcct gaccctgcca ggctaccgcc tgttagcgta aaccaccaca taactatgga
     1681 gcatctgcac atgaaaaccc gtacacaaca aattgaagaa ttacagaaag agtggactca
     1741 accgcgttgg gaaggcatta ctcgcccata cagtgcggaa gatgtggtga aattacgcgg
     1801 ttcagtcaat cctgaatgca cgctggcgca actgggcgca gcgaaaatgt ggcgtctgct
     1861 gcacggtgag tcgaaaaaag gctacatcaa cagcctcggc gcactgactg gcggtcaggc
     1921 gctgcaacag gcgaaagcgg gtattgaagc agtctatctg tcgggatggc aggtagcggc
     1981 ggacgctaac ctggcggcca gcatgtatcc ggatcagtcg ctctatccgg caaactcggt
     2041 gccagctgtg gtggagcgga tcaacaacac cttccgtcgt gccgatcaga tccaatggtc
     2101 cgcgggcatt gagccgggcg atccgcgcta tgtcgattac ttcctgccga tcgttgccga
     2161 tgcggaagcc ggttttggcg gtgtcctgaa tgcctttgaa ctgatgaaag cgatgattga
     2221 agccggtgca gcggcagttc acttcgaaga tcagctggcg tcagtgaaga aatgcggtca
     2281 catgggcggc aaagttttag tgccaactca ggaagctatt cagaaactgg tcgcggcgcg
     2341 tctggcagct gacgtgacgg gcgttccaac cctgctggtt gcccgtaccg atgctgatgc
     2401 ggcggatctg atcacctccg attgcgaccc gtatgacagc gaatttatta ccggcgagcg
     2461 taccagtgaa ggcttcttcc gtactcatgc gggcattgag caagcgatca gccgtggcct
     2521 ggcgtatgcg ccatatgctg acctggtctg gtgtgaaacc tccacgccgg atctggaact
     2581 ggcgcgtcgc tttgcacaag ctatccacgc gaaatatccg ggcaaactgc tggcttataa
     2641 ctgctcgccg tcgttcaact ggcagaaaaa cctcgacgac aaaactattg ccagcttcca
     2701 gcagcagctg tcggatatgg gctacaagtt ccagttcatc accctggcag gtatccacag
     2761 catgtggttc aacatgtttg acctggcaaa cgcctatgcc cagggcgagg gtatgaagca
     2821 ctacgttgag aaagtgcagc agccggaatt tgccgccgcg aaagatggct ataccttcgt
     2881 atctcaccag caggaagtgg gtacaggtta cttcgataaa gtgacgacta ttattcaggg
     2941 cggcacgtcg tcggttacag cgctgaccgg ctccaccgaa gaatcgcagt tctaagcaac
     3001 aacaaccgtt gctgactgta ggccggataa ggcgttcacg ccgcatccgg caatcggtgc
     3061 acgatgcctg atgcgacgct tgcgcgtctt atcatgccta cagccgttgc cgaacgtagg
     3121 ccggataagg cgttcacgcc gcatccggca atcggtgcac gatgcctgat gcgacgcttg
     3181 cgcgtcttat catgcctaca accgttgccg aacgtaggcc ggataaggca tttacgccgc
     3241 atccggcaat tctctgctcc tgatgagggc gctaaatgcc gcgtggcctg gaattactga
     3301 ttgctcaaac cattttgcaa ggcttcgacg ctcagtatgg tcgattcctc g
//
LOCUS       DAJOSY010000215         3342 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJOSY010000215 DAJOSY010000000
VERSION     DAJOSY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.215"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            511..1215
                     /locus_tag="OHN27_004345"
     CDS             511..1215
                     /locus_tag="OHN27_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HCQ2288659.1"
                     /translation="MARCTAPVRGHSSAAAAAACPACRNRSGYRRSSYSYDSYPSYSS
                     GSSGGGSYGGSSGGSSSSAKPRWSKSGSSVSYTTAQVQSLTPIRQAVEKLAIEQPDLR
                     DVFLCHAWDDRQGIAKELHDLLEAAGVKVWFSEKDLALGVPMMRAIDKGLANSRIGLV
                     LVTPALLNRLPKEGVADKELSALLAGNQLVPIVHNTTYEALRNVSPLLASRSGLDTGE
                     DTLAVVATKIAELITV"
     gene            complement(1639..2370)
                     /locus_tag="OHN27_004346"
     CDS             complement(1639..2370)
                     /locus_tag="OHN27_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCQ2288660.1"
                     /translation="MNQPIHNAYWLSRFESILNSALAQHRVVSLIRMDLRFPEYMPVT
                     IMDPDSDSAVISRFFESLKAKIQAYQRHKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSLGDFTVPSSLATLIKLAWCSALHLEPWQGNGLVHFSRRTPFRKPA
                     SSDARPSSDDTPLSGGCSDTRKASDKKSGGSAVLWVKRGDVEAMQKAMERACYLVKYE
                     TKQHDGSGQRNYGCSRGPGRLLDGR"
BASE COUNT         1039 a          780 c          679 g          844 t
ORIGIN      
        1 gattaaccga tcagatgctc aacggactca ataaattggc tgagtcaggt tacctcatcc
       61 acactctatt tgtttctaga aggatctacc attttgttca tctaccggaa cacatcaatt
      121 tagtaactcg ataatatcgg ttatgtagat atttatcgtt gctaccaatg cctagattat
      181 tattctccaa gtattttctt gacttacagg ctaattttga aacaaatgtc atgctatttt
      241 aggaaaagaa cctatgcaaa agtctgctat gagcgaggag cagatattca tctggtatac
      301 aaagacaata tcgcttaagt catcagtgct gccactttaa ccactagctc tgctcttttt
      361 ggacttttga gctaaaaatt aaatctgaca gtgtgataag gtgcaagttt ctgaaaatac
      421 gccagttgtt ttacactcaa aagcccccat tactatctga tgttaatggc gcagcagtta
      481 tctttcagat tattaagggg aaacaagagt atggcaagat gtacagctcc tgtacgtgga
      541 catagctcag cagcagctgc tgctgcctgt cccgcatgtc gtaatcgcag tggttatcgc
      601 cgcagtagct acagttatga tagttaccct tcttactcat caggctctag cggtggcgga
      661 agttatggag gcagtagcgg tggttccagc agcagcgcga agccacgttg gtcaaaatca
      721 gggtcttccg tttcttacac aacagcccaa gtacaatcac tcacaccaat tcgacaagcg
      781 gtagagaaac ttgccattga gcaacctgat ctgcgtgatg tctttctttg ccatgcttgg
      841 gatgaccgac aaggtattgc caaagagtta cacgatttgc tcgaagcggc aggtgtcaaa
      901 gtatggttca gcgaaaagga tcttgccctt ggtgttccga tgatgcgtgc cattgataaa
      961 ggattagcaa actctcgaat cggcttggtt ttagtgacac ctgcattgtt aaaccgtctt
     1021 cctaaagaag gcgttgctga taaagaactt tccgccttac tggcaggcaa ccaactcgtt
     1081 cccatcgtgc ataacacgac gtatgaagct ctccgtaatg tcagtccttt gcttgcatcc
     1141 cgaagcggcc tcgacacggg agaagataca ttggcagttg ttgcaacaaa aattgcagag
     1201 ctgatcactg tttagtttat ccaagccacc gttaaatatt tccagctcat gaatctggtc
     1261 tgctattgca ggccagatat cagtcaatac tgaaaggttt gacctgctcc ccgttgatta
     1321 acatagactg atatagcaac ttccgctttt ggcacaaagc agaccatgag aagatagacg
     1381 ctataacatt tgagtcctac caatcgatct gttcatttcc ataaatggca gtaaatgcaa
     1441 ataaccagaa cagccaggcg cttttcgact tatggtcgat gttgcaaaga gtgaggagtg
     1501 gaaagcaagc gtgcgaacga aagtagatgg caatccattg cattacttca ggacgacttt
     1561 tcagaaagcg agctaagggc gtcagccaac gcccttaccg tggcataagc tcaggcaccg
     1621 gatgccgttt tacagggctc acctgccatc cagtaaacgc cccggcccac gactgcaacc
     1681 ataattacgt tgaccagaac cgtcatgctg cttcgtctca tacttcacga gataacaggc
     1741 tctctccatg gctttctgca tcgcttccac atcaccacgc ttgacccaga gcacagcgga
     1801 tccaccagat tttttgtcag aagctttccg ggtatcagaa catccacccg acaaaggcgt
     1861 atcatcggaa gaagggcgag catcagatga tgccggttta cggaaaggcg tccgcctgga
     1921 aaaatgaacc agtccattac cctgccaggg ttcaagatgc agagcgctac accatgccag
     1981 cttgatcagc gtcgccagcg aagaaggaac ggtgaaatcc cctagcgagc accaggtatc
     2041 tttgttgagc agtaatatca cgtgataatg tttcctgcct ttttccttgc caaactcccg
     2101 acaccagaaa taacgcagag tggttgcacg cacacgcttg ttggcacgtc gtttatgtcg
     2161 ctggtaagcc tgaatttttg ctttcaggga ttcgaaaaaa cgagaaatca ccgctgaatc
     2221 cgaatcggga tccatgatgg tgacaggcat atactcaggg aaacgtaaat ccatccggat
     2281 taacgagacg acacggtgtt gcgccagggc actgttgaga atactttcaa aacgggataa
     2341 ccagtaagca ttgtgtattg gttgattcat gatgagagat ctccgttgat ttggattgac
     2401 ttcacatcat taatcaccgc ggtaaaactt tacggcatcc cgccattgag caacgccaat
     2461 ggctcctgat taaaacagaa tgaatccccc acgaagaaaa aataaaccta cacgcagagt
     2521 aaaacacaca acaacggcat aaatggtttt taaaaactac aaagcctgaa gggaaacgga
     2581 tccacagaac gtgcaaacaa aacaccaaaa acgcaatcag aacgctttct gctgctgtga
     2641 atcaacaaca ataccaaaac ctacagctta accacaaaac tcaatcacag agccgaatag
     2701 cgtgtttaaa acctgtttac agataaccct gccccgagaa caaaaaccac caacatcaga
     2761 atcgattttc ttatcctgaa aacgttactt ttcaacacac cataaaaacg attaaccatc
     2821 gataacgtaa acctcatctg aatcaaaaca ccagaaccaa tcatcacgct tttctgaaaa
     2881 gagccttatg atgaagataa tcaccttgca ctgagaaaaa acaccaggac cagttaatca
     2941 tctcatcaat ctcatcgtta caaaatcatc cataaaacca tttaacaaaa tcatcgtaag
     3001 cacacagtaa atcacgccag gttatctcag ccggatttgc atttttacat aaagacactt
     3061 gtctcaggtt aacgactaaa aacacaaaaa ctcagaaaag gttaaacgta acatcagtaa
     3121 aatcgaacag caggttgatt gcttaaacag gaaaaattgt tttgtagcag ctttaaactg
     3181 attttagcta acgtaaagat ttactaaact ggatgtgttt cagacaaagc aatgtgtaca
     3241 gcaatgctta atgaaaacac ttatagggtt gtttatgaaa acacgtgtat gtctggtaaa
     3301 acacttactg aaatgcttag taaaaatact tagtaaaata cg
//
LOCUS       DAJOSY010000216         3324 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJOSY010000216 DAJOSY010000000
VERSION     DAJOSY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.216"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            188..1708
                     /locus_tag="OHN27_004347"
     CDS             188..1708
                     /locus_tag="OHN27_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCQ2288661.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYKLVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVIGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1733..2071)
                     /gene="maoP"
                     /locus_tag="OHN27_004348"
     CDS             complement(1733..2071)
                     /gene="maoP"
                     /locus_tag="OHN27_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCQ2288662.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2190..3029
                     /gene="hdfR"
                     /locus_tag="OHN27_004349"
     CDS             2190..3029
                     /gene="hdfR"
                     /locus_tag="OHN27_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCQ2288663.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(3125..3200)
                     /locus_tag="OHN27_004350"
     tRNA            complement(3125..3200)
                     /locus_tag="OHN27_004350"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3165..3167),aa:Trp,seq:cca)
     gene            complement(3209..3285)
                     /locus_tag="OHN27_004351"
     tRNA            complement(3209..3285)
                     /locus_tag="OHN27_004351"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3249..3251),aa:Asp,seq:gtc)
BASE COUNT          888 a          819 c          816 g          801 t
ORIGIN      
        1 agtatgaatg ttgttccctt cctcgtagtt catcctttgt ccgctgcatt tttgcgagca
       61 tcattccacc gccagaacaa ctcaacttca cttgcacaaa taaacaggaa tcagccacta
      121 aaattcacga attagcgggc tacagcagcg atatcttatg cacataatgg tgactcaagg
      181 agggcttatg tcactgtcaa ttgttcatac ccgcgcagcc ctgggagtaa atgcgccccc
      241 gatcactgtt gaggtacata tcagtaaagg tctacccggc ttaacgatgg tgggcttacc
      301 agaaacaacg gtaaaagaag ctcgcgatcg cgtgcgcagc gccattatca atagcggata
      361 tgaatatccg gcgaaaaaaa tcaccatcaa cctggctcca gctgatctgc caaaagaagg
      421 gggacgatat gatttaccta tcgccattgc gttgctggcg gcctcagaac agcttacagc
      481 caataagtta gatgaatata aattagtcgg agaactggcg cttacaggcg ctctgcgtgg
      541 cgttcccggc gcaatctcca gtgcaactga agctattaag tcgggcagaa aaattatcgt
      601 cgcgaaagat aacgaagatg aagtggggct aattaacggt gaaggatgcc tgatagccga
      661 tcatctgcaa gctgtctgtg cgtttctgga aggtaagcac gctctcgaac gcccgaaacc
      721 aactgatgca gtatcccggg cgctacaaca tgatctcagt gatgttatcg gtcaggaaca
      781 aggaaagcga ggactggaaa ttaccgctgc tggcgggcac aaccttttac tgattgggcc
      841 gccgggaaca ggtaaaacaa tgctcgccag ccgtattaat ggcctgttgc cagatttaag
      901 caatgaagag gcactggaga gtgctgcgat attaagtctg gtaaatgctg aatcagtaca
      961 aaaacaatgg cggcagcgcc cgttccgctc acctcatcac agtgcatcgt taactgcgat
     1021 ggtaggcggt ggcgcaattc cagggcccgg tgaaatttcg ctggcgcata acggcgtgct
     1081 ttttcttgat gagctacctg aatttgaacg gcgtacactg gatgccttgc gagagccgat
     1141 tgaatccggg cagatccatc tttcacgcac acgagcaaaa ataacctatc cagcccgttt
     1201 ccagcttgtt gcggcgatga atcccagccc taccggacat tatcagggaa accataaccg
     1261 ctgcacgcca gaacagacat tacgttatct caaccggctc tcggggccct ttctcgaccg
     1321 cttcgatctc tcactggaga tcccattacc accccccggc attttgagta aaacggtagt
     1381 gccgggagaa agcagcgcca ccgttaaaca acgcgtaatg gccgccagag agcgccaatt
     1441 taagcggcag aataaactga atgcctggct ggatagtccg gaaatacgcc aattctgcaa
     1501 acttgagagc gaagatgcga tgtggttgga aggaacactg atccatctgg ggttatcgat
     1561 tcgtgcctgg cagcggttat tgaaagttgc acgaaccatt gctgatattg atcagtctga
     1621 cattatcaca cgtcagcatt tgcaggaggc agttagctat cgagcgattg accgtttgct
     1681 catccatctg cagaaactac tgacataaaa aaagggcatt tcgccctttt tattaatcgt
     1741 cagaatcggt gtagtcttca gcaccttcaa cctgcggttt accgccggaa agggtgtgaa
     1801 aacgttttgg acgcttaata cgcgtcatat acttggacca cacgcgttct gcttctgtca
     1861 ctggctcacg ttcgccacgg catactgcta cgaagagttt ctcttcctcg gtaaccggct
     1921 cgcgtttgcc aagatccaac tcattgaagg cataaccatg acgctcaagc agttgtgcct
     1981 ctttgatggt gaaatcacca tgacgagaga atccacgtgg ataatgttta ttgtcgaaat
     2041 atcgattagt cgtcgtaaag ctttccgcca tcctgcacgc tcctaattct ttgaccgagc
     2101 tagttatggc gcggagtatt agttacgctt gacagagtgt aaaacaaaac atttaaatca
     2161 taacgacaaa taattttgcg gagagcactg tggatacgga attgttaaaa actttcctgg
     2221 aagttagccg aacgcgtcac tttggtcgag cggctgaatc gctctatctg acccagtcag
     2281 cagtgagctt tcgaatcaga caactggaaa atcaactggg tgtgaacctt ttcacccgcc
     2341 acagaaacaa tatccgttta accgctgccg gtgaaaaact actgccttat gcagaaacgc
     2401 tcatgagcac ctggcaggcc gcccgtaagg aggtggcgca tacctcacga cataacgagt
     2461 tttctatcgg tgccagcgcc tcgttgtggg aatgtatgct taatcagtgg ctgggacgct
     2521 tgtatcaaaa tcaggatgcc catacaggct tacagttcga agcgcgaatt gcccaacggc
     2581 agtctctggt aaaacagctg catgaacgcc agcttgatct tcttattacc actgaagcgc
     2641 ccaaaatgga cgaatttagt agtcagttgc tgggatattt cactttagcg ctttatacca
     2701 gtgccccttc aaaactaaag ggagatctta attatctgcg acttgagtgg gggccagatt
     2761 ttcaacagca tgaggcaggt ttgatcggtg ctgacgaagt gcccattctg acaaccagtt
     2821 ctgctgaact ggcacagcaa cagattgcga tgcttaatgg ttgcacctgg ctacccgtca
     2881 gctgggcgcg taaaaaaggc ggcctgcata ccgttgtcga tagcacaaca ctttcacggc
     2941 cgctttatgc catatggctg caaaatagcg ataaaaatgc gttgattcgc gatcttttga
     3001 aaattaacgt gctggatgaa gtgtattaat atgaatggct ggcaaggatg ccggtagaag
     3061 gatttacttc ggagagggtt atttcagata aaaaaaatcc ttagctttcg ctaaggatga
     3121 tttctggcag gggcggagag actcgaactc ccaacacccg gttttggaga ccggtgctct
     3181 accaattgaa ctacgcccct aattagggtg gcggaacgga cgggactcga acccgcgacc
     3241 ccctgcgtga caggcaggta ttctaaccga ctgaactacc gctccaccga attcttttac
     3301 aaccaccggt tttatgaccg gctt
//
LOCUS       DAJOSY010000217         3289 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJOSY010000217 DAJOSY010000000
VERSION     DAJOSY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.217"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            159..1370
                     /gene="bluF"
                     /locus_tag="OHN27_004352"
     CDS             159..1370
                     /gene="bluF"
                     /locus_tag="OHN27_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCQ2288664.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1575..2306
                     /gene="bluR"
                     /locus_tag="OHN27_004353"
     CDS             1575..2306
                     /gene="bluR"
                     /locus_tag="OHN27_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCQ2288665.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2527..2931
                     /locus_tag="OHN27_004354"
     CDS             2527..2931
                     /locus_tag="OHN27_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCQ2288666.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2984..3094
                     /locus_tag="OHN27_004355"
     CDS             2984..3094
                     /locus_tag="OHN27_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288667.1"
                     /translation="MEIKVQRLSLWMINTVFLLSPINNHQTNTINLIFEM"
BASE COUNT          977 a          685 c          682 g          945 t
ORIGIN      
        1 ctgacaatct atacaagatt tttcgccaac tagtgttcaa ctttttccat tttttatcaa
       61 tgtaaagaaa cggttatgtt ttggtattaa aagaaagaca caagaagata tgtctgttac
      121 cgtcttactc tcgcctcacc cattaccctg gattgttaat gcttaccacc cttatttatc
      181 gtagccatat acgtgacgac gaacctgtca aaaaaatcga agaaatggtt tcgatagcaa
      241 atcgcaggaa catgcagtct gacgtaacag ggatcttact gtttaatggt tctcattttt
      301 tccagcttct ggaaggtccg gaagaacagg ttaaaatgat atatcgggct atatgccagg
      361 atccacggca ctataatatt gttgagctgc tgtgcgatta cgcgcctgct cgccgttttg
      421 gcaaagcggg aatggaatta tttgatttgc gcctgcacga gcgagatgac gttttacagg
      481 ccgtattcga caaaggcaca tcaaaatttc agctaactta tgatgacaga gcgctacaat
      541 tttttcgtac ttttgtcctt gcaaccgaac aatcaaccta tttcgagatc cctgccgaag
      601 actcctggct ttttatcgct gacggatctg ataaagaact tgattcctgt gccctttcac
      661 caactataaa cgaccacttt gcctttcatc ctattgtcga tcccttatcg cggcggataa
      721 tcgcttttga agccattgtg caaaaaaatg aagatagccc atcagccata gcggttgggc
      781 agcgtaaaga cggggaaatc tacacagcgg atctcaaaag taaggcgctt gcattcacga
      841 tggcacacgc acttgagctc ggtgataaaa tgatttcaat caatctatta cctatgaccc
      901 tggttaacga acctgacgca gtctcttttt tacttaatga aataaaggcc aatgctctgg
      961 tgcctgaaca aatcatcgtt gaatttactg aaagtgaagt catatctcgg tttgatgagt
     1021 ttgccgaagc gattaaatcg ctaaaggctg ctggtatcag tgtagcaatt gatcattttg
     1081 gcgcaggttt tgctggtttg ttactcctgt cacgcttcca gcctgacaga attaaaatca
     1141 gtcaggaatt gattaccaat gttcataaaa gtgggccacg gcaggcaatt attcaggcga
     1201 tcataaaatg ctgtacatca cttgaaattc aagtcagtgc tatgggcgtg gcaacaccag
     1261 aagagtggat gtggcttgaa tccgcaggaa ttgagatgtt tcagggagat ctgtttgcta
     1321 aagctaaatt gaatggtatc ccttccatag cgtggccaga gaaaaaataa ttttcagcac
     1381 attctttcac atgatttcag taaatcatcg tgaaaatgaa tacgttacgt ttaaatttaa
     1441 tccacattaa aaataatgtt tcatccaatt tagctgaaat cttatttata ctttgtacaa
     1501 ttttagcgta tattgtacaa agtattattg ggtcgtgtac aggcgacgga gatttgtgac
     1561 cgcaaggagg aattgtggct tattacagca ttggtgatgt tgctgaacgt tgcgggatta
     1621 atcctgtcac tctccgggcc tggcaacgcc gctacggttt gttaaaacca cagcgcagtg
     1681 aaggcggaca ccgactcttt gatgaagaag acatacaacg catcgaagag atcaagcgtt
     1741 ggataagtaa tggcgtccct gtaggcaaag ttaaagcatt actggaaacc accagccagg
     1801 atacggaaga tgactggagc cgcctgcaag aagagatgat gtcaattctt cgcatggcta
     1861 atcctgccaa actacgcgcg agaattattt cactgggtcg agagtaccca gtagatcaat
     1921 tgattaatca tgtttacctt cctgttcgcc agcgtctcgt gcttgatcac aacacctccc
     1981 gcattatgag cagtatgttt gacggcgcat taattgaata cgcagcaacc tcgcttttcg
     2041 aaatgcgccg taagcccggt aaagaagcca ttctgatggc gtggaatgtt gaagagagag
     2101 cacgattgtg gctggaagca tggcgtttat cattgtcagg atggcacatt tctgttcttg
     2161 ctgatcccat tgaatcgccg cgcccggaac tgttcccgac gcaaacattg attgtctgga
     2221 caggcatggc accaacgaga aggcaaaacg aacttttaca acactggggt gagcaaggtt
     2281 ataaagtcat ctttcatgcc ccctaatttc acctttatcc cgccccttag acagaatgtt
     2341 ataatctgaa cccacaacag cgtttgtacc ggagctggtc cgttgcaaaa tgctcaccgg
     2401 gagaattcag atattccctc tatcacactc tgtacatatc acagccaaag gacagaccag
     2461 attgaagtgg taaaaccgat cgtgcacact acgcagtctc acaactaaca agaggttatt
     2521 atcctgatgg atcaggtcgt tgtttttcaa aagatgtttg agcaagtgag aaaagagcaa
     2581 aacttctcct ggttttattc agaattaaaa catcaccgta ttgcacatta catttattat
     2641 ctggctacgg ataacatcag aattattact cacgatgata cggttttgtt attaagagga
     2701 accaggaacc tgttaaaagt tagtacgacc aagaaccctg ctaaaataaa agaggccgca
     2761 ttgcttcata tttgcggaaa atctacattt cgggaatact gttcaacact ggcaggcgcg
     2821 ggcgttttcc ggtgggttac tgatgttaat cataacaaac gcagttacta cgccattgat
     2881 aatacgcttt tatatattga agatgtagaa aataataaac cattaatcta gctaaagttg
     2941 gatacttaag aaatgcttca taattcagta aggcattagc ataatggaaa taaaagtgca
     3001 gagactatct ctatggatga ttaatactgt ctttttattg tcacccataa ataatcacca
     3061 gactaatact atcaacttga tatttgaaat gtgatcactt gacttttgat acgttatttt
     3121 ataacggtta acatatttat aaaaacaacg gccgtgccac acgtccgttt caatacttaa
     3181 cgcacatgta ttttggttta gtcatcatcc ggttatatgt attttagcca ggaacaggtt
     3241 aaatcattcc tatataactc aaaaattgaa accttattct catgtcatg
//
LOCUS       DAJOSY010000218         3244 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJOSY010000218 DAJOSY010000000
VERSION     DAJOSY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.218"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(29..2722)
                     /gene="chiA"
                     /locus_tag="OHN27_004356"
     CDS             complement(29..2722)
                     /gene="chiA"
                     /locus_tag="OHN27_004356"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HCQ2288668.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKVNDATNPWRLKRTATAAEISQYGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPSPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEISQYGNPGSCSVKPDNNGGAVTPVDPTPETPVTPTPDNSEPSTPADSVND
                     YSLQAWSGQEGSEIYHVIFNGNVYKNAWWVGSKDCPRGTSAENSNNPWRLERTATAAE
                     LSQYGNPTTCEIDNGGVIVADGFQASKAYSADSIVDYNDAHYKTSVDQDAWGFVPGGD
                     NPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPW
                     KPLGKAQSYSNEELNNAPQFNPETLYASDTLIRFNGVNYISQSKVQKVSPSDSNPWRV
                     FVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKD
                     ANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQH
                     YYDIVDNLNLKVLDFDIEGSWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPIL
                     PTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIAN
                     LHSQLKGLHPNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVG
                     IWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
     gene            complement(3014..>3244)
                     /gene="tuf"
                     /locus_tag="OHN27_004357"
     CDS             complement(3014..>3244)
                     /gene="tuf"
                     /locus_tag="OHN27_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2288669.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
BASE COUNT          807 a          838 c          751 g          848 t
ORIGIN      
        1 gcgatactgg aaggtatcgc caacacattt attgcttagt aaacggcgcg aagatttcgc
       61 taaatgcgta cttcggtgcc tgttctttag tcaggccgtg gaattccgga gacaggttag
      121 tgccgcccgg caggtcgcgc gcgattgacc agataccaac cataccgaga ttacgcttct
      181 gcgcatcctg catgaccaga cgagcatcag agagatagaa cacctcgccc tgaacgtcgt
      241 tcacgccaac catcggcgtg gtacccatca tagcgtcaat ttctgcatcg ctcttattgg
      301 gatggaggcc tttcaattgt gaatgcaggt tggcaatcgc agacgttgca cacttaccgt
      361 gaatgttctg gccttcggta tttgcagact gacaaatcgc gttaccgtag tccattgtca
      421 tcacgttcac acccgccagc tcaacacctt tcgctttggc atcgctcagg acattcatcc
      481 cttccggcgt caggccagtc ggcagaattg gcaaggtgta ccagatagca atatctttgc
      541 cttctgactt ccatttatcc tgcactttct tcacagcaag gttacgacgt tcaatagatg
      601 cctgatccgc aacccaggag ccttcgatat cgaagtccag gactttgagg ttcaggttat
      661 caacgatgtc ataataatgc tgcatcagat cgtctacgtt cttacaggaa gcagccagcg
      721 gagcgttgtt agcaccaccg atagacagca tcacatcgcc gccagcctca cgcagagctt
      781 tgattttgct gtactgagcg taattctgca taccgtaagc ggtaccccat gtcggcagac
      841 aggtgttcgc atctttactc accacaaacg ccagcgtgaa gtggttgacg ttatgattct
      901 tagccagcgc agccagatcc gggatcgtat tcagcgtaaa gtcgacatac ggtgcataaa
      961 cgtgtttcgg ccacgctttc ttcggcgtac ctacgcgctc tttggttccg gtccagtcaa
     1021 caaaaacacg ccacgggttg ctgtcagaag gagaaacttt ctgcacttta ctctgagaaa
     1081 tgtagttcac accgttaaag cgaatcagcg tatcgctggc ataaagcgtt tctggattaa
     1141 actgcggcgc attattcagc tcttcgttgc tatagctctg agccttacct aacggcttcc
     1201 acgggcggct attgctaccg gtggcgtttt ggttcgccac cagagcaggg ttgtcacttt
     1261 gcgtccagaa cagcgcttca taagcctgcc catcaacaac aacgcgatca cctttcacgt
     1321 acacagtgga tgcggaccat gctttcgccg gttcgtattt cttccacggg ttatcgccgc
     1381 ccgggacaaa gccccatgcg tcttgatcga cagaagtttt ataatgtgca tcgttataat
     1441 ctacgatgct gtccgcgctg tacgctttgc tggcctggaa accatccgca acaatgacgc
     1501 cgccgttatc aatttcacag gtagtcgggt taccgtactg actcaattcc gcagcggtag
     1561 ctgtacgctc gagacgccat gggttattgg agttttcagc gctggtaccc cgtgggcaat
     1621 ctttagaccc aacccaccag gcgttcttgt aaacattacc attgaaaata acatggtaaa
     1681 tttcgctacc ttcctggccg ctccacgctt gcaatgagta atcgttaacg ctatccgctg
     1741 gtgttgatgg ctcgctgtta tccggagttg gggtcaccgg tgtttccgga gttggatcaa
     1801 ccggagtcac agcaccgcca ttattatccg gcttaacgga acaggagcca gggttaccat
     1861 actggctgat ttctgttgcc gttgccgcgc gaacataacg ccatgggtta ctggcatcgt
     1921 tttctttcgc atcgcctggg caatttgccg cgcctaccca ccaggcgttc ttataaatgc
     1981 cgccgttata gataacgtac catgtagaag aaccttcctg acctttccag gctactacag
     2041 catagttgtc gtttgactta tcagctgggg ctggctgaac atcaggagcc ggtttaacgt
     2101 ccggtgccgg tttcacatcc ggtgaaggct gtggtgctga agtacaggac tgtggattac
     2161 tggtttctga gatttccgtt gccgtagcgg cacgaacata acgccatggg ttgctggcat
     2221 cattgccttt cgcatcaccc gggcagttag aagaggctac ccaccaggca ttcttatata
     2281 ccgcaccatt aaagacgaca taccaggtct gaccgccctg ctgtttattc caggcaacta
     2341 cagaagaatt agacggaaca gtgccctgtg ctggtgtagc cgaaccgcca ttagccggcg
     2401 tattggatgc aggcgtattt gaatttgaag aagatgagct gccgctcttt tcgcaggaaa
     2461 gtgtattgcc atactgacta atttcagcag ctgtcgcggt acgctttaaa cgccacgggt
     2521 tagttgcatc atttactttc gcttttcccg ggcaatttgt agaagaaacc caccaggcat
     2581 tttcataaat tttgccatcg aaaataacct gatatttatt accaccttgt tggttattcc
     2641 atgcttccat tgccaatgct ggcagagcgg aacacaccag ccccatacca atcatagatt
     2701 tagtaaatat atttaatttc attataatcc cttgtgacgt aaaaactgca aaacaaaagt
     2761 cctacgcagg aattatttac gttttacgag aacccttcaa tatgaattaa attacggcat
     2821 taaaaataag aaaaaagcct gacaaatgaa gcattttaaa aacagaaaca ttcatattta
     2881 aaatgttaaa ttgaattgat attttaaata tgaataattt attcgttctg acagtacgaa
     2941 taagatatgc cgtcaacaaa tgcaaaaagg gcgccgaagc gcccttttca attcaaaact
     3001 aattaacgtg taattagccc agaactttag caacaacgcc cgcgccaacg gtacggccgc
     3061 cttcacggat tgcgaaacgc agaccgtcgt ccatcgcgat cgggtggatc agggtaacaa
     3121 ccattttgat gttgtcgccc ggcattacca tctctacgcc ttccggcagt tcgatggtac
     3181 cagtcacgtc agtagtacgg aagtagaact gcggacggta gcctttgaag aacggagtat
     3241 gacg
//
LOCUS       DAJOSY010000219         3240 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJOSY010000219 DAJOSY010000000
VERSION     DAJOSY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.219"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(27..884)
                     /gene="repA"
                     /locus_tag="OHN27_004358"
     CDS             complement(27..884)
                     /gene="repA"
                     /locus_tag="OHN27_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCQ2288670.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEVAVAAARRSRVEWENRQRKKQGLDALGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNCN
                     YSRLATASP"
     gene            complement(877..951)
                     /gene="tap"
                     /locus_tag="OHN27_004359"
     CDS             complement(877..951)
                     /gene="tap"
                     /locus_tag="OHN27_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCQ2288671.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(1174..1434)
                     /gene="repA"
                     /locus_tag="OHN27_004360"
     CDS             complement(1174..1434)
                     /gene="repA"
                     /locus_tag="OHN27_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCQ2288672.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            complement(1718..1924)
                     /locus_tag="OHN27_004361"
     CDS             complement(1718..1924)
                     /locus_tag="OHN27_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCQ2288673.1"
                     /translation="MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRE
                     RLCELNIHRGNTVVQVTLAYEARK"
     gene            complement(2139..2588)
                     /locus_tag="OHN27_004362"
     CDS             complement(2139..2588)
                     /locus_tag="OHN27_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288674.1"
                     /translation="MEYDDVSELMIKREHNILIVGNSASHIDKFFKEYSIPSSKYYDF
                     TQINSDSDVERNENAVSFIRDAMNSEELTIIFNCVGWPDLGGGSAVSLFAMMARKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKSELTGGKRR"
     gene            complement(2743..>3240)
                     /locus_tag="OHN27_004363"
     CDS             complement(2743..>3240)
                     /locus_tag="OHN27_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCQ2288675.1"
                     /translation="LMQLHAAGIRAGDAERILSSGECWQRQKTLLTEREVRFMKGLFR
                     IVDMKRWYLCPQVRVADIVQLNGNIRPRSRQWWQLFRMVSQWHVDVVIVERRSFSIVA
                     AVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTGADQQS
                     PEHRS"
BASE COUNT          793 a          880 c          707 g          860 t
ORIGIN      
        1 gccggattat tcagaggagg tcaggttcag ggggaagctg tggccagccg gctgtaatta
       61 cagttacgtg acagaatcat gcgctccttc acacgacgct ccacttcgcg ttttactgcc
      121 tcacgattag cagtgaagcg cccttccgcg atttcgcgcg tcagctgccg tttcaccagg
      181 gtgacaatat cctgacgttc cctgtccgca tcacgacgcg cacgggcacg ctttattccc
      241 cgggacttaa gctctgtctg gtaactgcgg aaacgctcac gcacaaaacg ccaggctttc
      301 gctatcagtt catccatacc cagtgcatcc agtccctgct tcttgcgctg tctgttttcc
      361 cattccacac gactgcggcg cgcagctgcc accgccacct cagacacatc gagagcggca
      421 aacagtgccg gtgtgaacgt gatgtcggtc ggaatgtagc acccgataag cgggtcatat
      481 tccgtctgat aggtaatcag gcccagctct gcgaggaacg tcagggcgcg ggtggcacgg
      541 gtgatagaga gttttccggc agcagactcc gtcgccagtc cgcactcaat ggccagtgtg
      601 gtgatggagc actggacgcg gttggccagc gggtcatagt ggaaacacag cccctgcagc
      661 agcgcatcaa tggcccgtcg acgcagtacc ggcggcatgc gccggcgcag accacgcgaa
      721 cgggcgtgtg ccacatgaat ggcgaaatca aaacgggagg tgaagcccac cgccttttcc
      781 atcagttttt cgcaaaactt cagcgttccg gcaccttcac ggggtgtaaa taccggattc
      841 gggttcttta cctggcggta atacgtttgt tgaagatcag tcacaccatc ctgcacttac
      901 aatgcgcaga aggagtgagc acagaaagaa gtcttgaact tttcccggca tataactata
      961 ctccccgcat agctgaattg ttggctatac ggtttaagtg ggccccggta atcttctcag
     1021 tcgccaaact ttctgaagat tatcggggtt tttgcttttc tggctcctgt aaatccacat
     1081 cagaaccagt tccctgccac cttatggcgt ggccagccat aaaattcctt aaacgatcag
     1141 taatctagca tgctacgcca caaagtaaag tctttacttt agtatatcca gtctctgcag
     1201 ttcatctttg atgattttct caacgaactg agcctgcgtt atcccctctc tctcgcagta
     1261 ctcaaccatg agatctttca gaggattttt gacaaaaact tttatctctt tgtgtgtaag
     1321 acgttttctt gcaacagcag ccatttgttt ctcagagtca gtcataggct tacctctgcg
     1381 cacaaaccgc ttttgactca atgaggaagt cactgcattt tctgtctgcg acatctcgcc
     1441 tcctcaatac tcaaacaggg atcgtttcgc agaggatact acagtttttt gaaatcagcg
     1501 acttgagaat tgtgacgaag atctgtgttg gcgcacaaat caacagggat tactgtcgtt
     1561 taatgtgaat tttgttgcga aatggctgaa aaacaggaaa tgccttaacc tgtgtgccgg
     1621 ccccgggcgg gaccggcggt ttctgacgtg tagggtgtct gcccctgaaa tacctcacac
     1681 tcccggaaag cggggacttc cgtccccgcc cggcagctta cttccgtgct tcgtaggcca
     1741 gagttacctg caccactgta tttcccctgt gaatattcag ttcacataac cgttccctga
     1801 atatcagtga aaaacacaac accgtggcgc acacggcgag caacccgata agggcatatt
     1861 tcgtcataaa ctttgacctc tctgcaagga agaggctaca atcagtagtg ttcaagtact
     1921 tcatgcagac ctcacgagtt aatggattaa caagtggggt cttcgcattt ctggtacctg
     1981 cgtcttgcct gaaccagaaa ggctcagaca cccgccgcga taataccaca ttccctgttg
     2041 ctgactatta ccttcatttc ccgtttttta ctaccgaagc acgaaggcgt cactccagcc
     2101 aataagtatg ctttttattt acatctattt ttaatccgtt accgacgttt ccctccagtt
     2161 aactcgcttt tcgtatgact catggcaata aaattttctt taccatatga caacatccca
     2221 ataatatcaa agttgtcttt taatttgatg gcatcctttt cacttacagc gacaataagt
     2281 tgtttaccaa attttctggc catcattgca aacagtgata ctgctgatcc accacctaaa
     2341 tcaggccacc caacgcaatt aaaaatgatt gtgagctctt cactattcat tgcatccctg
     2401 ataaaagaga cagcattctc atttctctct acatctgaat cactgttaat ctgagtgaaa
     2461 tcataatatt tagaacttgg tatagaatac tccttaaaaa acttatcaat gtgggaggca
     2521 gaattcccaa caataagaat attgtgttca cgcttaatca tcaattctga tacatcatca
     2581 tattccatat attcaccttt attatataaa gtttgggtac aggtagttcc ggaatttatt
     2641 ctgtgaccac catttcccgt tttttattac ggaaacgccg ggagcgggga aaacttgctt
     2701 tttcccgttt ccggggttgg acaactgagc aacgcgaagg cgtcagctac gatgttccgg
     2761 ggactgctga tcagcccctg ttgtattcag ccattctccg gtcatctgca gcagttttct
     2821 ggcatcgtgg cttctgagca acggaatacc agcctgcctc agaacctctt ccagaagaat
     2881 atcccggcgt ctgcgttccg gtcgtaaatg gctggcatca tccagctcta ctgctgcaac
     2941 aatactgaac gaacgccgct caacgataac cacatcaaca tgccactgag acaccatcct
     3001 gaataactgc caccactggc gtgatcgtgg ccggatattc ccgttcagct ggacgatatc
     3061 cgcgacccgt acctgcggac acagatacca ccgcttcata tccacaattc tgaacagtcc
     3121 tttcataaaa cggacttccc tttctgtcag cagcgtcttc tgacgttgcc agcattcacc
     3181 gctggacagt attcgctctg catcaccggc ccgtatacct gccgcatgca attgcatcag
//
LOCUS       DAJOSY010000220         3219 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJOSY010000220 DAJOSY010000000
VERSION     DAJOSY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.220"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(89..616)
                     /gene="mobB"
                     /locus_tag="OHN27_004364"
     CDS             complement(89..616)
                     /gene="mobB"
                     /locus_tag="OHN27_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCQ2288676.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(598..1182)
                     /gene="mobA"
                     /locus_tag="OHN27_004365"
     CDS             complement(598..1182)
                     /gene="mobA"
                     /locus_tag="OHN27_004365"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCQ2288677.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMVF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1252..1521
                     /locus_tag="OHN27_004366"
     CDS             1252..1521
                     /locus_tag="OHN27_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCQ2288678.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1598..2584
                     /gene="srkA"
                     /locus_tag="OHN27_004367"
     CDS             1598..2584
                     /gene="srkA"
                     /locus_tag="OHN27_004367"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCQ2288679.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2601..>3219
                     /gene="dsbA"
                     /locus_tag="OHN27_004368"
     CDS             2601..>3219
                     /gene="dsbA"
                     /locus_tag="OHN27_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCQ2288680.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSE"
BASE COUNT          806 a          780 c          791 g          842 t
ORIGIN      
        1 aggccatcct gacggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc
       61 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      121 caaaatcagc cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg
      181 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      241 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      301 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt
      361 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      421 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      481 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      541 ttttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      601 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaacaa atgcatcttt
      661 atgatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatacca ttacccggcg
      721 ttctcctgct tgcagatatt ccagtaataa aggctcaata gcgcggttta ccagagcaat
      781 agtcgggtga tcgcgttcac cgtcatggac ccacacgaca ggcgcatctt tgcgctgatg
      841 attaagcctg gctgctaaat cagggggaat gtaaggcgta tcgcacgggc aaaacaaaaa
      901 ccactcacca gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
      961 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatgacgatt
     1021 agcattaacc acgacgtgag agagctgcgt cataagcgcg tcagcgacat gttgccataa
     1081 tggtttgccg tttaattcaa gcaatccttt atctacgccg cccattcgtc tggctttacc
     1141 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1201 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcatcac tatgaaatgt
     1261 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1321 aacctgctgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1381 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1441 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1501 cgcggcgtaa ttaaagagta aaagcttgta agcggcgcca ccaaaatcat cgtgaaatga
     1561 tatccttcgt cattcgtaat gttttccgga tgatgggatg aataacagcg cttttacttt
     1621 ccagacacta cacccggata ccatcatgga cgctctgttt gagcatggga tccgggtgga
     1681 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1741 tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1801 cgaagaacat caatttgcgt tgcagctggt aaatgatgaa gttccggtcg cagcacctgt
     1861 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     1921 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     1981 tttagggcgt atgcaccaga cggggcgcaa acagcttttt atccatcgcc cgaccatcgg
     2041 tttgaacgaa tatctcattg agccacgcaa gctgtttgag gacgctacac tgataccttc
     2101 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg attgccgccg ttacagcaca
     2161 ctggcgggaa gatttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2221 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagccgttca
     2281 ggatttgtgg atgttgctca atggcgataa agccgagcag cggatgcaac tggaaactat
     2341 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2401 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2461 cgcgttcccg aaaaatttcc cgtggttaac cggggaagat tactggctgc gacagacggc
     2521 gacttttata gaacaggcaa aagttctaca agaaccccct ttgcaattaa cacctatgta
     2581 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2641 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2701 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgcccg cactgctatc
     2761 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2821 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     2881 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt gactgttccg ctgtttgaag
     2941 gcgtacagaa aacccagacc attcgttctg cttctgatat ccgcgatgta tttatcaacg
     3001 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtg aaatctctgg
     3061 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggcgttccg gcgatgtttg
     3121 ttaacggtaa atatcagctg aatccgcagg gtatggatac cagcaatatg gatgtttttg
     3181 ttcagcagta tgctgataca gtgaaatatc tgtccgaga
//
LOCUS       DAJOSY010000221         3149 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJOSY010000221 DAJOSY010000000
VERSION     DAJOSY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.221"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..126
                     /locus_tag="OHN27_004369"
     CDS             <1..126
                     /locus_tag="OHN27_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCQ2288681.1"
                     /translation="LGALQKKLFIDGKRVGDREYAEVLIAITEYHQALLSRLMAD"
     gene            complement(414..1028)
                     /locus_tag="OHN27_004370"
     CDS             complement(414..1028)
                     /locus_tag="OHN27_004370"
                     /inference="COORDINATES: protein motif:HMM:NF025107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2288682.1"
                     /translation="MSTKNAQKEQKKESLITTHWGDDATIHGWTAIPNSLLMLQGDLG
                     IGSTEMCILLNILMHQWPESGESISFPSIGTIASRIGVSKRTIQRGVSNLEALGILTR
                     YQSTRNDPLTNGANIFDSTPLKEYLNKKAKGITISNSNKKKERKKTGITPNIKLPRIC
                     PKCLKTKATSHEEIVKFFGVRKTMAGEIINSYCKECRASEKDLF"
     gene            1357..2073
                     /locus_tag="OHN27_004371"
     CDS             1357..2073
                     /locus_tag="OHN27_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCQ2288683.1"
                     /translation="METEQQPQQTTEQIEVKTKRVQCFIDGYNLYHAIEETGNKALHW
                     VDLKSLCKRYLPKNASIDNVFWFSAKPSHLSRAINENYQNYTNALTECGVDVIGGRFK
                     KKNVTCEADNGCGGVFHKHEEKESDVNLAISLVSGACNDEYDIAIIITADSDLCPPIK
                     YVRERFPNKELWLVAPPGRIRRATDLCSLSSRSMEIKNDALRACCMRDEFYKDGVLVA
                     RRPQEWRAVANTRQRRARSR"
     gene            complement(2879..3073)
                     /locus_tag="OHN27_004372"
     CDS             complement(2879..3073)
                     /locus_tag="OHN27_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCQ2288684.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEELQNSIQTRFEAENRTGI"
BASE COUNT          785 a          657 c          765 g          942 t
ORIGIN      
        1 ctgggggcgt tacagaaaaa actctttatc gacggcaagc gtgtcgggga cagagagtat
       61 gcggaggtgc tgatagctat tacggagtat caccaggccc tgttatccag gcttatggca
      121 gattagcttc ccggagggaa gctgtcgaaa acagacggta tgaacgccgt aagcccccaa
      181 accgatcgcc attcactttc atacatagct atgcagtgag ctgaaagcga tcctgccgca
      241 tttttccggt ttaccccggg gaaaacatct ctttttgcgg tgtttgcggc agaatcgcgt
      301 tcagcgcgtt tctgcggtgc gcgtaatgag acgttatggt aaatgggagc gttagaaaca
      361 gtcccattta cttgagtggg gggcagttgg ccatataaaa aatggtcaac tgtttaaaat
      421 agatcttttt cagaagctct gcattctttg caatatgaat ttattatttc cccagccatt
      481 gtttttcgca cgccaaaaaa tttaacaatt tcttcgtggc tggttgcttt agttttcaaa
      541 cattttgggc aaatgcgtgg taattttatg ttgggtgtaa ttccggtttt ctttctttct
      601 ttctttttgt tgctgttgct aattgttatg cctttagctt ttttgtttaa atattctttt
      661 agaggagttg agtcgaatat attagcgcca ttggttagag ggtcattcct tgttgattgg
      721 tatctagtta atatccccaa tgcctctagg ttactaaccc cgcgttgaat tgttctttta
      781 gatacaccta ttcttgatgc aatggtacca attgacggaa aagatattga ctcccctgac
      841 tcaggccatt gatgcatcaa tatattcagc agaatacaca tctcagtact cccaatacca
      901 agatcacctt gcagcataag gaggctattt ggtatagccg tccacccatg tatggttgca
      961 tcatctcccc agtgagtagt gattaatgac tctttttttt gttctttctg tgcgtttttt
     1021 gtgctcatag atgcttttct cgcgaaaaaa aatcaaaatg tgaacagtta ctacctaact
     1081 actcttaata taagagtagt taggtagtaa catgtatctt ttaggatgac ataaatgtca
     1141 tccatttgct ctttttttat atagaactta agttgagtcc actacctaag agttatgctc
     1201 ttagtggcta tccaagtcga ggcttggaag cgcattgtac aaaattcttc ctgtattgga
     1261 tgcgtccata cagtggtctg ttaactgcgg ttaagagacg caaaaaggcc tgcatatgca
     1321 ggcctttttg ctatctagag gtcaaaaatg gcaagtatgg aaacagagca gcaaccacaa
     1381 caaactactg agcaaatcga agtaaaaaca aagcgagtgc agtgctttat tgatgggtat
     1441 aatttatatc atgcaattga agagactggt aataaagctc ttcattgggt tgatcttaaa
     1501 tctttatgca agagatacct tcctaaaaat gcatctattg ataatgtttt ttggttttcc
     1561 gctaagccat cacatttatc aagagccata aatgaaaatt atcaaaatta caccaacgct
     1621 ttaaccgaat gtggtgttga tgttatcggt ggacgtttta aaaaaaagaa tgtaacgtgc
     1681 gaagctgata atggatgtgg tggagttttc cataagcatg aagaaaaaga gtccgatgtt
     1741 aatttggcta taagtctagt gtctggtgcg tgtaatgatg agtatgatat agccattatt
     1801 atcactgcgg attctgactt atgcccccct attaaatacg taagagagcg attccctaat
     1861 aaagagcttt ggcttgtagc tcctccgggg agaatcaggc gagcgacaga tctctgctct
     1921 ctgtcatcga gaagtatgga gatcaagaat gatgctttga gagcctgctg tatgcgtgat
     1981 gagttctaca aagatggagt acttgtagcc agaaggcctc aggaatggcg agctgttgcc
     2041 aataccaggc aaagaagggc tagaagtcgt taggctttgt tgacgcagct agcgcaagga
     2101 gattaatcta actaggttga aaacgaaaaa accgccttgg cgggcggttt ttcgaaggtt
     2161 catagagttg gcgctctttg aaccgaggta actggctttg aggagcacag tcgccaaaac
     2221 ttgttctttc agtttagcct tatcaggtgc ataacttcaa gactcaactc ctctaaatca
     2281 gttaccaatg gctgctgcca gtggcgttaa gtcgtgtctt tccgggttgg actcaagacg
     2341 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2401 cttggagcga acgacctaca ccgaactgag ataccaacag cgtgagctat gagaaagcgc
     2461 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2521 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2581 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2641 gaaaaacggc atcggcgctg gcttgttccg gtgctttgct ttttgctcac atgttctttc
     2701 cggcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgacaccg
     2761 ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaagagcgtc
     2821 tgacgtggca ttttctcctt acgctccgct atgccgttct gcatcctgtc cgggtgcgtt
     2881 atatcccggt gcgattttcc gcttcaaagc gtgtctgtat gctgttctga agttcttccg
     2941 cgagttcgtg cagcttctca cacatggcgg cctgttcgtc agcatcaagt gcgtccagtt
     3001 tttcgagcag cgtcaggctc tggcttttta tgaatcccgc catgttgagt acggtttgct
     3061 gctgcttgtt catctttctg ttttctccgt tctttctgtc atctgcgttg tgtgattata
     3121 tcgagtacca cttttcgact gttttaccg
//
LOCUS       DAJOSY010000222         3102 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJOSY010000222 DAJOSY010000000
VERSION     DAJOSY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.222"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1480
                     /gene="traU"
                     /locus_tag="OHN27_004373"
     CDS             <1..1480
                     /gene="traU"
                     /locus_tag="OHN27_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HCQ2288685.1"
                     /translation="AQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDATWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            1480..2094
                     /gene="traV"
                     /locus_tag="OHN27_004374"
     CDS             1480..2094
                     /gene="traV"
                     /locus_tag="OHN27_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HCQ2288686.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRFQCFPLAPLPENRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            2061..>3102
                     /gene="traW"
                     /locus_tag="OHN27_004375"
     CDS             2061..>3102
                     /gene="traW"
                     /locus_tag="OHN27_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HCQ2288687.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELV"
BASE COUNT          830 a          818 c          782 g          672 t
ORIGIN      
        1 tgctcagaaa aacctgccct tcattgccta tatcgataag ggatacagtg ctcagggact
       61 ggttcagctt attcgcgaca gtcttcctcc ggaaagaaaa gacgaagctg tcggtattat
      121 tctgagcaat gatccggaat acacccgtaa cctttttgac gtgatgtacg gcgctaaaaa
      181 gcctattacg ccggaaaaga atttcatgtc atcggtgctg tgtgctctct gtgtcgatac
      241 cggtaccggt caaccctgta accccggaga cacccgacaa attatcaacc agcttatcga
      301 gctggctttt aaagagtacg gtgaaaataa tccgcgactt tatcgcgcct caacggaaga
      361 actggtggat tcagcactgc aggactcggg actgtatgaa aaacatgatg caacctggtg
      421 ggccagatca acctggttcg aagtccggga tatgctccac aatgccgggt atatcatggc
      481 ggctcagcgg gcacattatc aggcaatgcc ccagcttccc gaagtgtcct cgatgctggg
      541 ccataccagt ctcagggatg ttttcggtac tgttcagcgt gatggttcaa acgagcttct
      601 gctggactac atccgccgcg ccctggagca gggacataac gactacccca tgatatccgg
      661 gtatacccgg tttatgatca acccggaaac ccgggtcatc gctgtcgatc tgaataatgt
      721 tgccggtgac aaaacgcctg caggcaggct taaaaccggc atcatgtacc tgcttgcagg
      781 tcagatcgcc ggtggtgact tcaccctgcc acaatacagg gatgaagtgt taaagcagct
      841 tccacgggaa taccatgaaa tcgctcttaa acgtattaac caacttgatc aggaggttaa
      901 gacaaaagtt tatgatgaac tgcataacgc ccggggcatt gacttcatct gggaaaatct
      961 ggatacccag gagcgtgagc aacgcaagtt tgctatccgc acggttctga gtacgcagta
     1021 tctgagggac tacccggaaa gcgttcttaa gtcagcaaat actctctggt tactgcgcta
     1081 caaacccgaa gatatccccg tactgaggga taacttcaac gttcctgaat ttatgctgaa
     1141 acgcttcctg aaaatgccgg aaggcccggc accagatggt agtggtgttc ctgttctggg
     1201 agtattcagg gtaaaaagtg gcacgcttgc gagaatactc aaatttaccg tcggtccact
     1261 ggagctatgg gcgctgaact catcgccaaa agacagtgcc ctgcgtaaaa cactgacaaa
     1321 taagttaggt tcagttcggg cacgtaagat tctggccgaa aacttcccca gaggttctgc
     1381 aacgtcatta attgagcacc gggcgggcca acataacagt gacaatgtta ttgaagaact
     1441 ggcctcggag ctcatccgaa aacagggata caatctctga tgtgtacaat tcatatcacc
     1501 ccacgctgcc ggagacctgc cagagggcag gtatttctta aaaaacttga ggtaaaaggt
     1561 gacagtttct cattcactct gccgtcacgt gtcttcaggt tacatccggc ccccaatccc
     1621 agagtgatat acgcctcgga tgccagaaca cagggaaagg ttacaggcat gcggttgttg
     1681 ttgccgtcca gcacagatga gctgataaca acggcaatat ggcaggtttc tgtcccggat
     1741 gaagcaccat tcgacgttca gcatgtcatc cagtggatac tcccttcacc gctggcttcg
     1801 ctgatgtccg atgatacctg gttatggcca tccgttcccg gaacgccggc actgagtgcc
     1861 ggtgactggc ctgttatgga tactgaccct tccaccttac ggcgcgtcag acgtcgttct
     1921 ttcagcatca tcgataaccg gaacaggaaa ggttatatca ccaggcgatt tcagtgtttc
     1981 ccactggccc cactacctga aaacagaaaa tacgacagcc ttcctgacct tgtgctccat
     2041 cccgagcagg agacaataaa atgcaaagaa aaacattact cgcagccctt atagccaccc
     2101 tgagtggaac ggcatgccag gctcatgctt attcagtcac cgtcgtcgcc agccggcctg
     2161 ttgaagagca ggtcatcccc agaatggaag ccatcaaaga tgtccttggc aacatactga
     2221 gtacccagac agccacgggt accgctatca atcagaactc tgaaaagctg gcaagtgtca
     2281 ttgcccagaa cggacaggcg acacgtcagc agatgatttt cagtaatgaa actcagcgcc
     2341 tcgaggaggc acggaaaagc ttcaccgtgc cagactccat ctgcagtgaa tccgcatcag
     2401 ggattgcaac agaaagcaaa agtgcctcag catccgcagc ctcaaaactc agtaaaggtg
     2461 gcggtgtgag caaccgtagc atccgcgacc gtctggccag cgcagcaaac tctcccgtgc
     2521 gggaagctta tgatggcgcg gccatacacg ccagttactg tacagaagca gaatatgcac
     2581 gttttggagg aaccgccgtt tgtccttctg tgggcgaaat accaggtgga gacagtcagg
     2641 tacgctcgat ttaccacggc gcaggtacag cggacacacc tgcagcactg acatgggatc
     2701 agaagcaaat agatgcagcg acggcttaca tgaaaaacac ttcccgcccc tctgccggtc
     2761 gtgctctggg taaaggagaa gtgaataccc agagtgggcg aacctacgtc ggactccaga
     2821 acgaatacaa cggcatcatt gattctgcgt cgaatcccca actgacgctg attgctgaca
     2881 gcacgcctaa tgaaagcaca cgtaaagcac tggctgaaac actgcagtca gactcagcgg
     2941 ccgcttactt cgaccaggtg gcttcaccag aagcaaaagc acggggatat atgagtaccc
     3001 gtgagtttga agcttttgag gctggccggc gctacgccaa cacagcatac ctggtggatt
     3061 tacaggaaat gcagggagat aacctgctcc gtgaactggt aa
//
LOCUS       DAJOSY010000223         3072 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJOSY010000223 DAJOSY010000000
VERSION     DAJOSY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.223"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            156..371
                     /gene="bdm"
                     /locus_tag="OHN27_004376"
     CDS             156..371
                     /gene="bdm"
                     /locus_tag="OHN27_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCQ2288688.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            473..610
                     /gene="sra"
                     /locus_tag="OHN27_004377"
     CDS             473..610
                     /gene="sra"
                     /locus_tag="OHN27_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCQ2288689.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            767..2464
                     /gene="maeA"
                     /locus_tag="OHN27_004378"
     CDS             767..2464
                     /gene="maeA"
                     /locus_tag="OHN27_004378"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCQ2288690.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGMV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2598..>3072
                     /locus_tag="OHN27_004379"
     CDS             2598..>3072
                     /locus_tag="OHN27_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCQ2288691.1"
                     /translation="MKAAVVTKDHHVDVTYKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSK"
BASE COUNT          830 a          742 c          782 g          718 t
ORIGIN      
        1 cacaatccct atgaatatca tggtgttaaa atataaaaat catcaaccag gactaatctt
       61 aacaacgaag cggcaaatat ttgccatgct gaatgtgctt tataaatctg cgatccgtag
      121 cagacaccat aaatacacag acacggagaa tcactatgtt tacttattat caggcagaaa
      181 attcaacagc agaacccgct ctggttaatg ccattgagca aggccttcgg gcacagcatg
      241 gtgttgtcac tgaagatgac attctcatgg agctgaccaa atgggttgaa gcttctgata
      301 acgacatcct cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc
      361 ctacgcttta aggtgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc
      421 ttcgctacgg cgatgttgtc cttaaaacta gctacaggat tgaggagtta aaatgaaatc
      481 gaaccgtcag gcacgtcata ttcttggact ggaccataaa atttctaacc agcgcaaaat
      541 agttaccgaa ggtgacaaat ccagcgtagt aaataaccca accggcagaa aacgccccgc
      601 tgaaaagtaa ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat
      661 ggtgtttttg tttttatctg ctttatactt gaggccgacg ccctggcggt aaagcaaaga
      721 cgataaaagc cccccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac
      781 aaaaaaacag cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt
      841 gttgaataaa ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt
      901 actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca
      961 gggattcaaa accgaaatcg acaaacacat ctacctgcgt aacatccagg acactaacga
     1021 aaccctcttc taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac
     1081 cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt
     1141 gtttatctct taccagaacc ggcacaatat ggacgatatt ctgcaaaacg tgccgaacca
     1201 taatattaaa gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg
     1261 catcggcggg atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat
     1321 cagcccggcg tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacagctgct
     1381 taacgatccg ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga
     1441 attcgttgat gaatttatcc aggctgtgaa acaacgctgg ccagacgtgc tgttgcagtt
     1501 tgaagacttt gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg
     1561 ttcttttaac gatgacattc agggcactgc ggcggtaaca gtcggcacac tgatcgcagc
     1621 aagccgcgcg gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc
     1681 agcgggatgc ggcattgccg aaatgatcat ctcccagacc cagcgcgaag gattaagcga
     1741 ggaagcggcg cggcagaaag tctttatggt cgatcgcttt ggcttgctga ctgacaagat
     1801 gccgaacctg ctgcctttcc agaccaaact ggtgcagaag cgcgaaaacc tcagtgactg
     1861 ggataccgac agcgatgtgc tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat
     1921 tctgattggc gtctcaggac agaccgggct gtttacggaa gagatcatcc gtgagatgca
     1981 taaacactgt ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc
     2041 cacaccgcag gacattatcg cctggaccga aggtaacgcg ctggtcgcca cgggcagccc
     2101 gtttaatcca gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt
     2161 tattttcccg ggcatcggcc tgggtgttat tgcttccggc gcgtcacgta tcaccgatga
     2221 gatgctgatg tcggcaagtg aaacgctggc gcagtattca ccattggtgc tgaacggcga
     2281 aggtatggta ctgccggaac tgaaagatat tcagaaagtc tcccgcgcaa ttgcgtttgc
     2341 ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa acctctgccg aagccctgca
     2401 acaggccatt gacgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat
     2461 ctaagcctgc gcccggtagt gaaggctacc gggctatttc cctctccctt tttcagatct
     2521 catccatact gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca
     2581 tccgaaaagg aggaactatg aaggctgcag ttgttacgaa ggatcatcat gttgacgtta
     2641 cgtataaaac actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg
     2701 gtgtatgtca taccgatctt catgttaaga atggcgattt tggtgacaaa accggcgtaa
     2761 ttctgggcca tgaaggcatc ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa
     2821 aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact
     2881 gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg
     2941 gcgggatggc ggaagagtgc atcgtggtcg ccgattacgc ggtaaaagtg ccagatggtc
     3001 tggactcggc ggcggccagc agcattacct gtgcgggagt caccacctac aaagccgtta
     3061 agctgtcaaa aa
//
LOCUS       DAJOSY010000224         2946 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJOSY010000224 DAJOSY010000000
VERSION     DAJOSY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.225"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            20..268
                     /locus_tag="OHN27_004380"
     CDS             20..268
                     /locus_tag="OHN27_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288692.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            600..2123
                     /locus_tag="OHN27_004381"
     CDS             600..2123
                     /locus_tag="OHN27_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCQ2288693.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            2255..2473
                     /locus_tag="OHN27_004382"
     CDS             2255..2473
                     /locus_tag="OHN27_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288694.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
BASE COUNT          804 a          593 c          626 g          923 t
ORIGIN      
        1 atatttctac gaggctgaca tgaataattc aatcccagag agatttattt ttcaatgtgc
       61 tttgtttaaa aatctcgaga gggaggtgtt catgacgcat gggtatgttg atagtcatat
      121 tattgatcag gctttacgct tgcgtttaaa agatgaaacc agtgtgattc tttctgatct
      181 ctatctgcaa atattgcagt acattgaaat gcataagact acactaacgg atatcattat
      241 taatgacagg gaatccgtgc tctcttagaa cttgagtaag gttttagaat aaacatcagc
      301 tgtatcacca tgctgatgca aagtgagcat ttcaggcgtt atgctttctt attatgtccg
      361 caatatcagg tgtcaagaat ggagagttct cgctctccat tcttgacgcc tgatatcccg
      421 cctaacttat tttgtactgc ctacattatg ctcaataatt aatgggttag atcaagatgg
      481 agaaaatttt atcttcggcg tggatatttc ttatgctctc taccatgctc tctgtagatg
      541 ccagcgcgag cgaatgaatg gcattttcat gtattaaacc ctttggccgg gacaagcaaa
      601 tgcgcaatac actcataccc atcctcgttg cgatatgctt gtttattacc ggtgtcgcta
      661 ttttgaacat ccagctctgg tattccgcaa aagcagagta cctggcggga gcgagatatg
      721 ccgccaacaa tatcaatcat atacttgaag aagcgtcaca agcgactcaa acagcggtta
      781 acattgccgg gaaggaatgc aacctcgagg agcaatatca gcttggcact gaagcagctc
      841 tgaaacctca cctgcgcaca atcatcattc tcaaacaggg aatagtctgg tgtacatccc
      901 tgcctgggaa tcgggtcctg ttgtctcgta ttcctgtttt cccggacagt aatttactgt
      961 tggctccagc aatcgacacc gttaatagat tacctatcct gctctatcag aaccaatttg
     1021 cagatacgcg cattttggtt acgataagcg atcagcatat tcgtggggca cttaatgtac
     1081 ccttgaaagg ggtaaggtat gtattacgcg tggcggatga cattattgga ccaacgggtg
     1141 atgtgatgac gcttaatgga cattatccct ataccgagaa ggttcactcc acaaaatatc
     1201 atttcactat tatctttaac ccgccgccac tctttagctt ctacagactt atcgataaag
     1261 gctttgggat tttaatattt attctgttaa tcgcctgcgc cgctgccttc ctgcttgata
     1321 gatatttcaa taaaagcgca acgcctgaag agatcctgcg acgggcaata aataatgggg
     1381 agatcgtccc tttttaccaa cctgtggtaa atggtcggga agggacattg cggggagttg
     1441 aggtgttagc ccgctggaaa caacctcacg gtggatatat atcacccgcg gcatttattc
     1501 cacttgctga aaaatccgga ttaatcgttc cgcttacgca aagcctgatg aatcaggttg
     1561 ccagacagat gaacgctatc gcgagtaaat tgccggaagg ttttcatatt ggaattaatt
     1621 ttagcgcttc gcatattatt tcgccgacgt ttgtcgacga gtgtttaaat ttccgtgaca
     1681 gttttacccg ccgcgattta aaccttgttc tggaagtcac cgagcgtgag ccattgaatg
     1741 ttgatgaaag tctggttcag cggttgaaca tactgcatga aaatggtttt gtcatcgcac
     1801 tggatgattt cggtactggc tactcagggc tttcttatct tcatgacctg catattgatt
     1861 atatcaaaat tgatcacagt ttcgttggcc gcgtaaacgc agacccagaa tcaacccgaa
     1921 ttctggattg tgtattggat ctggcgcgta aactttcgat cagtatcgtc gctgaaggtg
     1981 tcgaaacgaa agaacaactt gactatctga accaaaataa tatcacattt cagcagggtt
     2041 attatttcta taaacctgtt acatacatcg acctggtcaa aattatcctt tctaaaccga
     2101 aggtgaaggt tgtggttgag tgaaaattga tcagtaaggc catagtgcgg tgtaattata
     2161 gacagctaat tagctcgttg cctcttgtta ctattgttca ttattttgtt tgctataatt
     2221 gtttgaaagt tttgacagga ttgccattag tagcatgaac aatagtaata atctggatta
     2281 tttcactctc tatatcatat tttccattgc atttatgctg atcaccctcc tggtcatcct
     2341 tattgcaaaa cccagtaccg ggctgggaga agtgcttgtg acgataaatt tgcttaatgc
     2401 ccttgtttgg ctggcgatca atctggttaa tcgattaaga gaaagactcg tcaaccacag
     2461 ggatcagcaa taatctttca gtttctcact gtcagtatgc ggctgaatgg gttgctggca
     2521 gtgaacgcct ggatcattga aggaaaggca ttattgcgca aatagttgtc aaccctggtg
     2581 ttatcacggt tgtttttata tatcaccgaa ataatcctca tcgcaactat taacaatttt
     2641 gatgtcgaag agttatttgt taaacaaaat cgtcacctca aagtgatcaa tgtcatgaaa
     2701 ataaggtgaa aaatgataat gccgacttat ttatcattta tatattgtcg ctgtttatct
     2761 atttaaattt tttgtgcttt tgtttttgtc tttaccctcc gttttggtaa tctgagctca
     2821 taggttattt gaccaggaat gtttgttaat ccaatgaatg gatggttaat ctatgaaact
     2881 gaaaaaactc cccgggttta gtttgggact tattgctctg gcggtgggta atgcatatgc
     2941 aacaca
//
LOCUS       DAJOSY010000225         2896 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJOSY010000225 DAJOSY010000000
VERSION     DAJOSY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.227"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            150..2429
                     /gene="ydeP"
                     /locus_tag="OHN27_004383"
     CDS             150..2429
                     /gene="ydeP"
                     /locus_tag="OHN27_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCQ2288695.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2677..2874
                     /gene="safA"
                     /locus_tag="OHN27_004384"
     CDS             2677..2874
                     /gene="safA"
                     /locus_tag="OHN27_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCQ2288696.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          789 a          665 c          695 g          747 t
ORIGIN      
        1 taatctattg tttaacgata atagattaaa aatagaaaga gagtagtccc ctactcttat
       61 atatccatgt tggcgataat ccctttgtat gtactgtgca tcatcgctat tacaaatcct
      121 aataattcat ttccacacag gataagtaga tgaagaaaaa aattgaatcc taccagggtg
      181 ctgcaggtgg ttggggtgct gtcaaatccg tagcgaatgc agtacgtaag cagatggata
      241 tacgccagga tgttattgcc atgtttgaca tgaataagcc agagggcttt gactgtccgg
      301 gttgtgcatg gccagatcct aagcacagtg cgtcattcga catttgtgaa aacggcgcaa
      361 aagcaatcgc ctgggaagtc acggataagc aggtaaatgc ctctttcttt gctgagaata
      421 cggttcaatc attacttacc tggggagatc acgagcttga ggctgcggga cgtctcactc
      481 agcctttgaa atatgatgcc gtcagcgact gttacaagcc attaagctgg caacaagctt
      541 tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc caatcaggtt gaattctata
      601 cttcgggccg cacttccaat gaagctgcct ttctttatca gctttttgcc cgtgaatacg
      661 ggagcaataa ctttcccgac tgctccaaca tgtgccatga accgacaagc gtgggtttgg
      721 cagcgagtat cggtgtaggt aaagggaccg tgttgctgga agactttgag aagtgcgatt
      781 tagtcatttg cattgggcat aaccctggta caaaccaccc tcgcatgctg acttcgttgc
      841 gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa tcctctacag gaacgtggcc
      901 tggagcgatt taccgcaccg caaaacccgt ttgaaatgct gacgaactct gagactcagt
      961 tggccagtgc ctactacaac gtgcgcattg gtggcgatat ggcgttgctc aaggggatga
     1021 tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc aggtcggccc tcattgcttg
     1081 atgacgaatt tattcaaacg cataccgtcg gctttgacga gctacgccgt gacgttctca
     1141 attccgagtg gaaagatatc gaacgtattt ctggactaag tcagacacaa atcgccgaac
     1201 tggctgacgc atatgccgct gccgaacgca ccattatctg ttacggaatg gggatcactc
     1261 agcacgaaca tggtacccag aacgtacagc aactggtcaa tttgctgttg atgaaaggta
     1321 acattggcaa gcctggtgcg ggtatctgcc cactacgtgg tcactctaat gtacagggcg
     1381 accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc ctgggtgagc
     1441 gctatggctt caccccacct catgcacctg gacatgctgc aattgccagc atgcaagcaa
     1501 tatgtacggg gcaggctcga gcattgatct gcatgggggg caactttgca ctggcaatgc
     1561 cagatcggga agcgagcgct gtaccgttaa cgcaattaga tttggcggta cacgtagcca
     1621 ctaagcttaa ccgctctcat ctgctgaccg cacggcatag ctatattctg ccggttctgg
     1681 ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc ggtaaccgtt gaggattcaa
     1741 tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc cggtgtaatg ctgaaatcag
     1801 agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg gtagcctggg
     1861 agtatctggt ggaagattat gatcgcattc gcaatgacat tgaagctgtg ctgccagagt
     1921 tcgccgacta taaccagcgc atccgtcatc ccggtggttt tcacctgata aatgcagctg
     1981 ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt cattaccagc aaagggctgt
     2041 tagaagatcc ctcttcagcg tttaacagta agctggtcat ggcgacagta cgcagccacg
     2101 atcagtacaa cacgacgatt tatggtatgg atgatcgcta tcgaggggta ttcggtcaac
     2161 gagatgtggt ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa aacggcgaaa
     2221 gagttaatct tattgcgctt acgccagacg gtaagcgcag ctcacgccgc atggatagat
     2281 taaaagtggt catttaccct atggctgacc gctcactggt gacctatttt ccagaatcga
     2341 atcacatgct aacacttgat aaccacgatc cattaagtgg cattcctggc tataaaagta
     2401 ttccggttga attagaacca tcaaattaat gcctcttctc atttcttctg ctgtcatccg
     2461 cacagcagaa gaattcctca ttgactatta tcgcgcaatt tgctcacatg gattaaatta
     2521 aactacatac tataagatat aaacttctgc ctacagctgt aagaaactcc gctcagtact
     2581 gaagcaccag tcctatttcc tcttttctcc agcctgttat attaagcata ctgattaacg
     2641 atttttaacg ttatccgcta aataaacata tttgaaatgc atgcgaccac agtgaaaaac
     2701 aaaatcacgc aaagagacaa ctataaagaa atcatgtctg taattgtggt tgtcttatta
     2761 ctgacactta cgttgatagc cattttttcg gcaattgatc agctgagtat ttcagaaatg
     2821 ggtcgcattg caagagatct tacacatttc attatcaata gtttgcaaga ctgaaaataa
     2881 acagcaaatt ataaat
//
LOCUS       DAJOSY010000226         2882 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJOSY010000226 DAJOSY010000000
VERSION     DAJOSY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.228"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(342..1535)
                     /gene="tyrB"
                     /locus_tag="OHN27_004385"
     CDS             complement(342..1535)
                     /gene="tyrB"
                     /locus_tag="OHN27_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCQ2288697.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1788..2867)
                     /gene="alr"
                     /locus_tag="OHN27_004386"
     CDS             complement(1788..2867)
                     /gene="alr"
                     /locus_tag="OHN27_004386"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCQ2288698.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
BASE COUNT          758 a          767 c          699 g          658 t
ORIGIN      
        1 ttaacattag aatttaacga tactataatt gaatctcaga aagtcacagg caataaggca
       61 aagcatattc ttgaggatat agaaatattc tatcgtaaca aacccgatac ttattattaa
      121 atttaataat ggttagatgt gcagaaatta ttactccacc caaaaaaaca ggataaggaa
      181 ttccagataa ttgggataaa cttaacaata aaagaactaa caacgtttat tatctctcac
      241 gtagaacgat ggcatcaaaa aaatcatcta taaagaaaga gtgaaagcga cgacagttta
      301 atttcactgc aggctgggta gctccagcct gctttcctgc attacatcac cgcagcaaac
      361 gcctttgcca cacgttgtac atttgccgta tttaacccgg cgacacacat gcgaccgctg
      421 gcgatgagat agacaccaaa ttcttcacgt agtcggtcaa cctgagcggc acttaaaccg
      481 gtataactga acatgccgcg ctgattaagc agataatcga aattgcgttc tggcatctct
      541 gtgcttaata ccttcaccaa ttcctgacgc attgccagaa tgcgagtacg catctcttct
      601 acttccgcca gccagctggc tttcaatgcc tcgtcattca gcactgcagc caccacctgc
      661 gcaccaaaat tcggcgggct ggagtagttg cggcgaactg ttgctttcaa ttgccccagt
      721 acgcggccag cggcttcggc atcttcacac ataacagaaa gtccgccgac gcgctcgccg
      781 taaagggaga aaattttcga gaacgaattg ctcaccagag cgggtaatcc agcgctggca
      841 atggcgcgaa tagcgtaggc atcctcttcc ataccggcac caaatccttg ataggcaata
      901 tcgaggaatg gaataagctc gcgggctttg agaatttcaa tcaccgcatc ccactgatca
      961 ttagtgagat cggcacccgt tgggttgtgg caacatggat gcagcaacac aatactgcgg
     1021 gcaggtaatg ttttcagcgt cgccaacagg tcattaaagc gcacgccgtt agtcgcttcg
     1081 tcataccagg ggtaagtact cacttcgaat ccagccccgg cgaatattgc tacgtggttt
     1141 tcccaggtag gatcgctgac ccagacgcct gattccggga agtagcgttt caggaaatcc
     1201 gcgcccactt tcaatgcccc ggagccgcca agggtttgaa tggttgctac gcgctgttgt
     1261 ttcagtaccg gatggtccgc accaaacagc agcggcgcaa tggcatggcg atagctgtta
     1321 agcccttcca tcggtaaata aagcgaagcg ccatgaggct gcgcattcag gcgcgcttcc
     1381 gcctccgcca cggcttgcag ttgtggaata attccgtctt cgttgtagta cagaccgata
     1441 cttaaattca ctttgtcgct gcgagggtct tctttaaaac gctccataag cgtaagaatc
     1501 gggtcgccag cgtaggcgtc aactttttga aacacgcgat ggttctccag gtttacgggc
     1561 aggtggttaa aacacaataa accggaagaa ggcgaagatc gagtggatgt tcaggagcga
     1621 acggcaatta gcaacagagt gagactcatg acaaacgtac atccgccaga ggcacgacct
     1681 ttataagagc atgaaagaac atcaacttat tgaattttta ggattttatt ggccggataa
     1741 ggcattcacg ccgcatccgg cacagacaat caaatattac agaacgatta atccacgtat
     1801 ttcatcgcga cccttgaagt caggcgcgta ataagttcgt aagcgcttac tttcgtcatt
     1861 tcagcgatac gttctacggg caaaccttcg ccccataaaa tgaccggatc cccggctttg
     1921 tcctgcgcct gtggacctaa gtctacgcag atcatatcca tcgccacgcg cccgacaatc
     1981 ggtacttcgc gaccgttcac cagcactggc gtaccggacg gcgcggcgcg cggataacca
     2041 tcgccatagc ccatcgcgac tacgccaaga cgggtatcac gttcgcttac ccaggttcca
     2101 ccataaccaa caggctctcc ggctttatgc tcacgcacgg caatcaggct ggaggttagt
     2161 gacatcactg gctgacagcc aaaatcggca ccggtggagc gatcttccag cggcgagacg
     2221 ccataaagaa tgatgcccgg gcgcacccag tcaaaatgcg actgtggcca cagcagaatg
     2281 ccacccgacg cggcaatgga acgttgacca ggtttgcctt cgcaaaaggt attaaagata
     2341 gcgagttgtt tctcggttgc gccacatttt ggttcatccg cgcgcgcaaa atggctgacg
     2401 atattcaccg gctgacgaac gtttttgcac tgggtcaggc gatgataaaa cgcctcagcc
     2461 tgttccggcc ttacgcccag acggtgcata ccggtatcga gtttcatcca gacggtaacc
     2521 ggctcgtcca ggctagcctc ttccagcgca gccagctgtt cttcgttatg cacggcggta
     2581 tgaaaatgtt gcgcagaaat cgtcggcaga tctctggcat caaaaaagcc ttcgagtaac
     2641 agtacaggtt tggtgattcc ccccgcacgc agtcgcagag cttcttcgag acgggctacg
     2701 ccaaaggcgt cagcatcggg gagcgttcgc gcggtctcaa gaagaccgtg accataagcg
     2761 ttcgctttca ccaccgcaac cattttactg gcaggggcca gttcacgaag acgttgcagg
     2821 ttgtgtcgca gagcgcggcg gttaatcaca acagttgccg cttgcatttg tgttccttga
     2881 ta
//
LOCUS       DAJOSY010000227         2876 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJOSY010000227 DAJOSY010000000
VERSION     DAJOSY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.229"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            15..308
                     /gene="iss"
                     /locus_tag="OHN27_004387"
     CDS             15..308
                     /gene="iss"
                     /locus_tag="OHN27_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HCQ2288699.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITFGIYTPLEARVYCSQ"
     gene            complement(366..541)
                     /locus_tag="OHN27_004388"
                     /pseudo
     CDS             complement(366..541)
                     /locus_tag="OHN27_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            1119..2218
                     /locus_tag="OHN27_004389"
                     /pseudo
     CDS             1119..2218
                     /locus_tag="OHN27_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          818 a          674 c          655 g          729 t
ORIGIN      
        1 tgcaggataa taagatgaaa aaaatgttat tttctgccgc tctggcaatg cttattacag
       61 gatgtgctca acaaacgttt actgttggaa acaaaccgac agcagtaaca ccaaaggaaa
      121 ccatcactca tcatttcttc gtttcgggaa ttggacaaga gaaaactgtt gatgcagcca
      181 aaatttgtgg cggtgcagaa aatgttgtta aaacagaaac tcagcaaaca ttcgtaaatg
      241 gattgctcgg ttttatcact tttggcatct atactccgct ggaagcccgg gtatattgct
      301 cacaatagtt gcccatcgat atggggagct catctgcact gttcattaat atacttctgg
      361 gctccctaca gttgtttttg catagtgata agcctctctc tgagggagga aataatcctg
      421 ttcagcgatg tctgccagtc gggggggctg cattatccac gcccgaggcg gtggtggctt
      481 cacgcgggga tgggcagatt gatctgatat gcaaccgacg acgaccagcg gcaacatcat
      541 cacgcagagc ttcattttca gatttgggcc accttttgat ttctcgttta acttttgcag
      601 tcaccagact gtgatttatt ttaacaaatc aatagctttc ataaaattct cgaagctgaa
      661 agcttaactg aaccaaccag cctggctggt gttttctaaa gacaaaaagg ccatcagcaa
      721 aacactgata gtctgaatca cctttatcaa tcatgtatga agaaaatatg cgcaattagt
      781 tgacagtgat tatcattttc attaaaaaga gcgagtagca ttcttatttc atgaggaaat
      841 ttacccgcca acaacctgag tagcgaaagc tgttcatccc caatattttt gctggcgggt
      901 tcttttttct acagtcctcc tccccagagg aagcattccc attaatcatt actggcgagg
      961 aggcaagaga aaaagtcggt tctttgtccc taaataacag catcatttca gtccatgcaa
     1021 aatctgattt accagctcac ataataacct gatgatacac agtgtaagtt cacagagata
     1081 ttgcaattgc ctccggataa gtaaggggag attgcactat gcaaatgcag cacctgaggt
     1141 tggctatcct aagcagccga ttatgcgttg aggctttcag tgatggctga tatagcaaca
     1201 atgagaatga aggctctgca cttctgggat aaacacggta tttctgcagc ttctgaagcc
     1261 tttggcgtat cctgccgcac gctttactgg tggcgtcagt tactgaacaa gggaggacct
     1321 gaggggctaa tcccgcacag taaggcacct ctggtgcgac gaaaaaagca ctggcatccc
     1381 gatgtgctga aagagattcg acgcctcagg acagagctgc cgaacctcgg taaagagcag
     1441 atttttgttc gcctgaagcc ctggtgcgca taacgccatc agacctgccc gagtgtttcc
     1501 accatcggca gaatgatcgc caccgcacac gataaaatgc ggatgatacc agtacgtctg
     1561 agctcgcggg gtaaggctct gcttgtcaaa aatcgaacca ccaaaccccc gcaggccaaa
     1621 gcactaccgt ccggtaaaga caggagagct cattgggatg gacgccattg agctcagaat
     1681 gggtgaaatg cgtcgctata tcattaccat gatcgacgaa cacagcgatt atgtactggc
     1741 gctggctgtg ccgtcgctca acagtgatat cgtcaatcac tttttcagcc gtgcagcccg
     1801 gctgttccct gtcggtatca gccagatcat cacagataac gggaaagagt tcctgggaag
     1861 ctttgacaaa acgctgcagg aagccgccat caaacacctc tggacctatc cctacacgcc
     1921 aaaaatgaac gctatccgtg aacgttttaa ccgggcatta agagagcaat tcattgaatt
     1981 taatgaaatt ttactctttg aggatctggc gttgtttaac ctgaaactgg cggaatatct
     2041 ggcgctgtat aacagcaaaa gactccacaa ggcgctcgca ctaacgacgc ccgtggaata
     2101 tattttaaaa gagaacaaaa attgcaatat gtggtggacc catacacagc aaacgcacag
     2161 gccgccccgg ttgggtagat atgtgggatg gctggttcag acttcaggat atggttgagg
     2221 ctggtgatga agtctcttta tcaggagatc tgatcaagag acagccgcgc agggggattc
     2281 actatgctgg attcttttaa caaattctcc gctaaccaaa aaatagactt tttgcattcg
     2341 taaatgcgtt aagagataat ttttaaagtt ccatcaatct cccggccagt tcgtctcgcg
     2401 gcattactca ctatccgcac aacttcagta agatcatgac tttttcaatc gttatctgtt
     2461 gaccaagctt tgaaaccact agcgtcccgt caacctgacc tggaatcgtc gagaaccgcc
     2521 catagttgtg aattggctag agtgtgatgg aaagcaacgc taaacgacct agtcacacat
     2581 tctcttttcc ctaacaatga ccatttagtg aaataacgta tccaaaatga tgacgtgaaa
     2641 gcctgacact ggcagcgaaa agccgaaaaa actcacctgg tcaggagcaa aaatcggatt
     2701 attcgccaac agcgtaatat cagcagtagt tctcgacacg cttgcgctat agacagagga
     2761 tgaaacgctg ctggtcggag ttctctatac atttttggcg acggcagaac cgctcaccaa
     2821 tgccttaacg tctatcagcc ttccggtgag ttggaacagc atgctcagcg tttgca
//
LOCUS       DAJOSY010000228         2870 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJOSY010000228 DAJOSY010000000
VERSION     DAJOSY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.230"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1786
                     /gene="bglX"
                     /locus_tag="OHN27_004390"
     CDS             <1..1786
                     /gene="bglX"
                     /locus_tag="OHN27_004390"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCQ2288700.1"
                     /translation="EGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGA
                     VEGGKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDV
                     LRDQWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGL
                     IKSGKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEARE
                     VARESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIK
                     NAVGENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVV
                     VAVVGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQ
                     ADAILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNA
                     DKPNKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTG
                     KREGATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQM
                     KYDAEPGKFNVFIGTDSARVKKGEFELL"
     gene            2038..>2870
                     /gene="osmF"
                     /locus_tag="OHN27_004391"
     CDS             2038..>2870
                     /gene="osmF"
                     /locus_tag="OHN27_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCQ2288701.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     Q"
BASE COUNT          725 a          715 c          813 g          617 t
ORIGIN      
        1 cgaaggtttt ggcgaagata cgtatctcac ctcaacaatg ggtaaaacca tggtggaagc
       61 gatgcagggt aaaagcccgg cagatcgcta ctcggtgatg accagcgtca aacactttgc
      121 cgcatacggc gcggtagaag gcggtaaaga gtacaacacc gtcgatatga gtccgcagcg
      181 cctgtttaat gattatatgc cgccgtacaa agcggggctg gacgcaggca gcggcgcggt
      241 gatggtggcg ctgaactcgc tgaacggcac gccagccacc tccgattcct ggctgctgaa
      301 agatgttctg cgcgaccagt ggggctttaa aggcatcacc gtttccgatc acggtgcaat
      361 caaagagctg attaaacatg gcacggcggc agacccggaa gatgcggtgc gcgtggcgct
      421 gaaatccgga atcaacatga gtatgagcga cgagtattac tcgaagtatc tgcctgggtt
      481 gatcaaatcc ggcaaagtga cgatggaaga gctggacgat gctgcccgcc atgtactgaa
      541 cgttaaatat gatatgggat tgtttaacga cccatacagc catttggggc cgaaagagtc
      601 tgacccggtg gataccaatg ccgaaagccg cctgcaccgt aaagaagcgc gtgaagtggc
      661 gcgcgaaagc ctggtgttgc tgaaaaaccg tctcgaaacg ttaccgctga aaaaatcggc
      721 caccattgcg gtggttgggc cactggcgga cagtaaacgt gacgtgatgg gcagctggtc
      781 cgcagccggt gttgccgatc aatccgtgac cgtactgacc gggattaaaa atgccgtcgg
      841 tgaaaacggt aaagtgctgt atgccaaagg ggcgaacgtt accagtgaca aaggcattat
      901 cgatttcctg aatcagtatg aagaagcggt caaagtcgat ccgcgctcgc cgcaagagat
      961 gattgatgaa gcggtgcaga ctgcgaaaca atctgatgtg gtggtggctg tggtcggtga
     1021 agcacagggg atggcgcacg aggcctccag ccgtaccgat atcactattc cgcaaagcca
     1081 acgtgacttg attgcggcgc tgaaagccac cggtaaaccg ctggtgctgg tgcttatgaa
     1141 cgggcgtccg ctggcgctgg tgaaagaaga tcagcaggct gatgcgattc tggaaacctg
     1201 gtttgcgggg actgaaggcg gtaatgcaat tgccgatgtg ttgtttggcg attacaaccc
     1261 gtccggcaag ctgccgatgt ccttcccgcg ttctgtcggg cagatcccgg tgtactacag
     1321 ccatctgaac accggtcgtc cgtataatgc cgacaagccg aacaaataca cttcgcgtta
     1381 ttttgatgaa gctaacgggg cgctttatcc gttcggctat ggtctgagct ataccacttt
     1441 caccgtctct gatgtgaaac tttctgcgcc gaccatgaag cgtgacggca aagtgacggc
     1501 cagcgtgcag gtgacgaaca ccggtaagcg cgagggggcc acggtagtgc agatgtactt
     1561 gcaggatgtg acggcttcca tgagtcgccc tgtgaaacag ctgaaaggct ttgagaaaat
     1621 caccctgaaa ccgggcgaaa ctcagactgt cagcttcccg atcgatattg aggcgctgaa
     1681 gttctggaat caacagatga aatatgacgc cgagcctggc aagttcaatg tatttatcgg
     1741 cactgattcc gcacgcgtta agaaaggcga gtttgagttg ctgtaatgct tttgtaaggg
     1801 cgcgacgttt gcgccctttg cctgatgttc atatattaac ttgctgaatt ttcgcccttt
     1861 gtaggccgga taaggcgttt acgccgcatc cggcaagaaa cgccgggcaa aatatgccct
     1921 ttaagtattt atctgattct gcaactgaat ccttccgctc aggctaactc tgtcattatc
     1981 aactatgctt ttctcttaat tcgctgaaaa caacagtaaa agaatgagga aagcatcatg
     2041 ccactctcaa aggtctgggc aggttcactg gttttgttgg cagccgtgag cctgccgctg
     2101 cacgcggctt cccccgttaa agtcggttca aaaatcgata ccgaaggcgc gctactcggc
     2161 aacatcatct tgcaggtact cgaaagccac ggcgtaccga cggtgaataa agtgcaactc
     2221 ggaacgactc ctgtggtgcg cggggcgatt acttccggtg aactggatat ctatccggaa
     2281 tataccggca atggcgcttt cttctttaaa gatgaaaacg atgcggcatg gaaaaacgcg
     2341 cagcaaggtt acgagaaagt caaaaaactc gatgcagagc aaaacaagtt aatctggctg
     2401 acgcccgcac cagcgaataa cacctggacc atcgccgtgc gtcaggatgt ggcagagaaa
     2461 aacaaactca cttcgcttgc tgacctgagt cgttatctga aagagggcgg caccttcaaa
     2521 ctggcagcct cggcagagtt tatcgaacgc gccgatgcct tacccgcgtt tgaaaaagcc
     2581 tacgacttta aactcgatca ggatcagtta ctgtcactgg ctggcggcga cacggcggta
     2641 acgattaaag ccgctgccca gcaaacttct ggcgttaatg ctgcaatggc ttacggcact
     2701 gacggcccgg tagcggcgct ggggctgcaa accttaagcg atccgcaagg tgtgcaacct
     2761 atctacgcgc ctgcaccagt ggtgcgtgag tcggtgttga aggagtatcc gcaaatggca
     2821 cagtggctac agccagtctt cgccagcctc gatgcaaaaa cattgcagca
//
LOCUS       DAJOSY010000229         2813 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJOSY010000229 DAJOSY010000000
VERSION     DAJOSY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.231"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..204)
                     /locus_tag="OHN27_004392"
     CDS             complement(<1..204)
                     /locus_tag="OHN27_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCQ2288702.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASE"
     gene            complement(666..1262)
                     /locus_tag="OHN27_004393"
     CDS             complement(666..1262)
                     /locus_tag="OHN27_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288703.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDDAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            complement(1259..1978)
                     /locus_tag="OHN27_004394"
     CDS             complement(1259..1978)
                     /locus_tag="OHN27_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCQ2288704.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            complement(1975..2409)
                     /gene="psiB"
                     /locus_tag="OHN27_004395"
     CDS             complement(1975..2409)
                     /gene="psiB"
                     /locus_tag="OHN27_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCQ2288705.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMRKVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPAAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(2464..>2813)
                     /locus_tag="OHN27_004396"
     CDS             complement(2464..>2813)
                     /locus_tag="OHN27_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288706.1"
                     /translation="CTACSLNGVQTRECGRTSRSPLDSLESAIGFHMRDWWQPTKANF
                     FGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKDNRWVPGWMCSPRPQT
                     DATERADNLADAA"
BASE COUNT          619 a          848 c          764 g          582 t
ORIGIN      
        1 ttcagaggcc atattccccg ggaatccctc ataatcctga aacatcagtt caggatcggc
       61 ttcatcctgg tgaagagcac ggcaggcggc gaaaaagtcg cgctcatcat caaacgtggt
      121 caggtcaaac cagcgtccgg cgatgcttcc acagttgtat ttgtgccagg ttccaacgta
      181 tacagcaggt gcaacaacag acattcccga atcctccata gtcacatgtt ctgcgacacc
      241 gggcggtgcc gtgcctccat aaaagccggg cgataaggcg gggatgcagg ggcgcaaggg
      301 aagccgcagc ctgacgcaca cggctgacgg gagttgcagg gtgaggccgc gaaacctgag
      361 cggacgaacg accggaaaat cacggcagac gggaaggagg gaccgggtga ccgcagcggc
      421 gaagcgtacc cttgcatccc tgccagcccg gctaagatgg aggcacggca cagcccgcac
      481 acgacgctga ggccacagag ctgaaacccc acaaggccat gccccgcatg gccgggtaaa
      541 agcggagccg ccggcgactt ccttattccg gcgagtgcag aggacacgcc gcatgacctc
      601 cagaccaccc ctgatatgaa acgatcatga tcggcgtgtc cctgcgtaaa atatacatgc
      661 ctggttcacc ggagaatatc tgtgatatcc acaagttttc tgtcgcgcag ccagtcctgc
      721 gcttcacgca ccacacaact gtccggtttc accgctttca gcgatgatgc cagtcgcttg
      781 atcaacatca ccagttcccg tgtctgtatg tccggcattg tctccggcgg agcaggggga
      841 ctctcatttc cgacgctcat cccatgcgct tccagtgtgg cacggacaaa ccaggcgtca
      901 tcgaccagtt gctgtccaat ccaggccaga tcggcatttc tgtcacgggc tgccattcgc
      961 tcgcccgaca gcgccttgct gatatggctg cgcaccagat tatccaggag tgcctgcgct
     1021 tcttccggtg aaaagccacc tggtatctct gtgcgcaaca gccctttcct gtacagaagc
     1081 tgcagcagat caacctgcga ccgcacagca actatcagta cataacggac aacattcgca
     1141 ctggcagtgg tttctgtatc attcagcagt tcttccggat cgttcccccc ggtgttgcag
     1201 agcaacggca acagaggtcg catccacgcg ggaacctggt atttttcgtc tgatttcatc
     1261 atattacgtt ctccagcttg ttcgggatca tccactgctc aagtgcacgt acctgtaccg
     1321 gtgcaccacg accggcctca ccggcttcaa aaatcagccg ccagagtggt tcctcctgcc
     1381 agaaaaaccg gtcaaatgat gacagtgatg gaaaacgccc gcaccacccc cagaaaatcg
     1441 tttccagatc atgctcctca acaacaccag accagcggga caaccagctt ccgaccgtgg
     1501 cgggagtatg aaacgcaagg tcaatctgcg cctgagcaaa acatgtctgg tattccagtt
     1561 cccgtcgttc tgcctcacgt tcccgctggc ggctgtattt ctgctctgtc agcgtggttt
     1621 tctgacgacg acgctcacct tcacgaaaag gcacggacgg gaacagacgg tgccccgcat
     1681 cggatggcag gggaagaaca cagacttccc caaaactttc aatcagttta tccacggcag
     1741 ccagccacag gagacggttg ccatgaaatt cctccttcgt cagcgcaggg gcaaaaaagc
     1801 ggagatcggt cagcgaaatc cggcgactgc cattcaggca gcgaaagaaa gcggtggcat
     1861 aaggccatgc tgaaagtgtg cgtccctgac gacgacgttg ttctgtgaca gccaccgcct
     1921 gcatggcggc ctgttgttct gcgctgaggg ggatcagtgc ccgtgaacgt gcgctcatgc
     1981 ctgacctcct tctgccatca ggatatggat gatgtcgtta acactgtatc cctgcgcatc
     2041 cagtgtcgcg gcaagcgcca gcgtgtgact gatcgcggca ggcgtgaaga tatgttgcac
     2101 ctgaacgacg gcgaaaggct gtccaccacc attcaccaga accagcatcc agagctgaga
     2161 gacgtctccc ggcgaacata atgccaggtg gaaacgttcg tgggctggcg tgaaacgaac
     2221 ctggacgggg aaaaagcccc cgaactcgct gccatactct ccgttcatca tccagttagc
     2281 cggtgcgtcc actttacgca tcacctcgtg agtgagatta cggcgcatat cgcttcccgc
     2341 agcacggatt tcttcatatt cctgggcatt catggactgc agggcattca gtgtcagttc
     2401 ggtattcatc atcgtatttc tccctctgcc gccccgtctc cggcggggcg gtgtggttgt
     2461 tcatcaggcg gcatcagcca ggttatcggc gcgttcagtg gcatccgtct gtggacgtgg
     2521 tgaacacatc cagccaggca cccagcggtt gtctttcata tggaactctg catgttcagc
     2581 ggcatcgcct ttcttcatct tctctgcgtc ccgtgcggca ccggacagtc cggcatcatt
     2641 cagggctgcg ataatctgcg gctttttcag gtgtccgaag aagtttgctt tcgtcggctg
     2701 ccaccagtcg cgcatgtgaa aaccgatggc gctctccagt gagtcaagcg gactgcgtga
     2761 cgtgcgacca cactcgcggg tctggacacc gttaaggctg catgcggtgc aga
//
LOCUS       DAJOSY010000230         2783 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJOSY010000230 DAJOSY010000000
VERSION     DAJOSY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.232"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            200..1366
                     /gene="nhaA"
                     /locus_tag="OHN27_004397"
     CDS             200..1366
                     /gene="nhaA"
                     /locus_tag="OHN27_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCQ2288707.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1432..2331
                     /gene="nhaR"
                     /locus_tag="OHN27_004398"
     CDS             1432..2331
                     /gene="nhaR"
                     /locus_tag="OHN27_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCQ2288708.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2369..>2783)
                     /locus_tag="OHN27_004399"
     CDS             complement(2369..>2783)
                     /locus_tag="OHN27_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCQ2288709.1"
                     /translation="FEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSD
                     VEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSNYYSFQHPH
                     EGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          654 a          621 c          706 g          802 t
ORIGIN      
        1 ccttaagcta tgattatcag ggcttagggt cacccgttag cgcttacagc cgtcaaaaac
       61 tcatctcacc gctgatggcg caaatccttt aattgctcgt aaaaaacgag ttattcctac
      121 actataatct gattttaacg atgattcgtg cggggtaaaa tagtaaaaac gatctattca
      181 cctgaaagag aaataaaaag tgaaacatct gcatcgattc tttagcagtg atgcctcggg
      241 aggcattatt cttatcattg ccgctatcct ggcgatgatg atggccaaca gcggcgcaac
      301 cagtggatgg tatcacgact ttctggagac gccggttcag ctccgggttg gttcactcga
      361 aatcaacaaa aacatgctgt tatggataaa tgacgcgctg atggcggtat ttttcctgtt
      421 agtcggtctg gaagttaaac gtgaactgat gcaaggatcg ctagccagct tacgccaggc
      481 cgcatttcca gttatcgccg ctattggtgg gatgattgtt ccggcattac tctatctggc
      541 ctttaactat gccgatccga ttacccgcga agggtgggcg atcccggcgg ctactgacat
      601 tgcctttgca cttggtgtgc tggcgctgtt gggaagtcgt gttccgttag cgctgaagat
      661 ctttttgatg gctctggcta ttatcgacga tcttggggcc atcattatca tcgcattgtt
      721 ctacactaat gacttatcga tggcctctct tggcgtcgcg gctgtagcaa ttgcggtact
      781 cgcggtattg aatctgtgtg gtgtacgccg cacgggcgtc tatattctgg ttggcgtagt
      841 gttgtggact gcggtgttga aatcgggggt tcacgcaacc ctggcggggg taattgtcgg
      901 cttctttatt cctttgaaag agaagcatgg gcgttctcca gcgaagcgac tggagcatgt
      961 gttacacccg tgggtggcgt atctgatttt gccgctgttt gcatttgcta atgctggcgt
     1021 ttcactgcaa ggtgtcacgc tggatggctt gacctccatt ctgccattgg ggatcatcgc
     1081 tggcttgctg attggcaaac cgctggggat tagtctgttc tgctggttgg cgctgcgttt
     1141 gaaactggcg catctgcctg agggaacgac ttatcagcaa attatggcag tggggatcct
     1201 gtgcggtatc ggttttacta tgtctatctt tattgccagc ctggcctttg gtagcgtaga
     1261 tccagaactg attaactggg cgaaactcgg tatcctggtc ggttctatct cttcggcggt
     1321 aattggatac agctggttac gcgttcgttt gcgtccatca gtttgacagg acggtttacc
     1381 ggggagccat aaacggctcc cttttcattg ttatcaggga gagaaatgag catgtctcat
     1441 atcaattaca accacttgta ttacttctgg catgtctata aagaaggttc cgtggttggc
     1501 gcagcggagg cgctttattt gacaccacaa accattaccg gacagatccg ggctctggaa
     1561 gagcgcctgc aaggcaaatt atttaagcgc aagggacgtg gtctcgaacc cagcgagctg
     1621 ggagagctgg tctatcgcta tgccgataaa atgttcacct taagccagga aatgctggat
     1681 atcgtgaact atcgcaaaga atccaattta ttgtttgacg ttggcgtggc tgatgcactt
     1741 tccaaacgcc tggtcagtag cgtacttaac gccgcagtgg tagaaggcga gcccattcat
     1801 cttcgctgct tcgaatccac ccacgaaatg ctgctggagc aattaagtca gcataaactg
     1861 gatatgatca tttctgactg tccgatagac tctacgcagc aggaaggcct gttctccgtg
     1921 agaattggcg aatgtggcgt gagtttctgg tgtacaaatc caccaccaga aaaaccgttc
     1981 ccggcttgtc tggaagaacg gcgacttttg attcctgggc gacgttcaat gttagggcgc
     2041 aaattgctta actggtttaa ctcccaggga ttaaacgtag aaatcctcgg cgagtttgat
     2101 gatgccgcgt tgatgaaagc ttttggtgcg atgcacaatg caatcttcgt tgccccaacg
     2161 ctttatgcat atgactttta tgccgataaa actgtcgtag aaattggtcg cgtcgagaat
     2221 gtgatggaag agtaccatgc tatttttgct gagcggatga ttcagcaccc ggcggtacag
     2281 cgaatctgca atacggatta ttctgcgctt tttagtccag cggcgcgtta atcggcagct
     2341 cccccaaagt taaagtggag gagatagatt agttgtacat aaccacgatt ttgactcggc
     2401 tcattatttg cccgcttgag acattatttc catatgtacg cgggcgaata aatagaggaa
     2461 tctgattact tccttcatgg ggatgctgaa aagagtagta attgctttta ccatactgga
     2521 tttcattcgt accatcattg gagagttgaa agcctacttg acccgcaccg tcctcactgt
     2581 tttctaactc attttttaat atgctggttc ctgactcctg tatcgtcact ggttcaaagt
     2641 aatattcaac atcactgggt tcaaaaacta attcaccttg ctcgccctgg atgcagtcaa
     2701 atcttatatg atgaaactga taattttcat tattgatacg agacagtaca aatcgtccag
     2761 tgtcgatgga ttgatcttca aaa
//
LOCUS       DAJOSY010000231         2721 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJOSY010000231 DAJOSY010000000
VERSION     DAJOSY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.233"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            19..573
                     /locus_tag="OHN27_004400"
     CDS             19..573
                     /locus_tag="OHN27_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCQ2288710.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(583..2010)
                     /locus_tag="OHN27_004401"
     CDS             complement(583..2010)
                     /locus_tag="OHN27_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCQ2288711.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENTGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYAQSWRETEPDNEDAPYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLLVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHTLWPLYLVAKQLVQACQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2007..2714)
                     /locus_tag="OHN27_004402"
     CDS             complement(2007..2714)
                     /locus_tag="OHN27_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCQ2288712.1"
                     /translation="MDMESQKILFALSTPMGIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
BASE COUNT          779 a          647 c          606 g          689 t
ORIGIN      
        1 aaatcgaagc attcacacat gaataaaaaa ttaatgtata tattcgcaat ttttatagtt
       61 gcagcaatta cctgtattag ccaacccaag aaaacgacgt tgcgtgataa agccatggtg
      121 aattatgcct ttgattattt aagctcaccg ggcagtcttc cattcaccac ggcagccacg
      181 gagctttccg cgattcatgg tcactcaacg tcgcaatatc gccttggaga attttatctt
      241 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
      301 gcggaacagg aaaatccacg cgcgcaaagt aaactggggt ggatctacct caaaggtctg
      361 ggggtcaaac ccgacacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
      421 tatgctcatg ctcaatatac tttaggtttg atctacagaa atggctcagg tattaatgtt
      481 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg
      541 gaaagattac ttgccgggct tcccgcacat taacgtcgac tgtcacccga acagatggag
      601 tattttccct atcaaaccag caaagataaa aatatagaaa ataattttaa tgatgcccag
      661 acagcctggc ccaccatcgt caacgggcat tggctgcggt tgcggctccg gttcaacagt
      721 ttgcttctcg gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag
      781 tggacaacga ttttctgcat caaggcggct cacaatcggc attaatgatt cttctgtagg
      841 ttggcaggcc tgcactaatt gtttggcaac tagatataat ggccataagg tatgttctgt
      901 atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct cgaccagtga
      961 acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg
     1021 ggcgctgtcg ccggggacat aaagtagcgg ttttccctga tcgttaacca gatcacgcgc
     1081 ttcaatcatc atcaccagta atgggtaata atcgacccga tgctgacggc accattgcaa
     1141 cattaaagca tccgcctggg tggagggata ctcgcgccag tagtcacaaa gttctgccaa
     1201 caggctttcg ccttctccac aatagacgcg ttgcgcgtat tcataataag gcgcatcttc
     1261 attatcaggt tcagtttccc gccatgactg ggcgtaatcc agaagttcgg ggatatccag
     1321 gcgacctgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggataat
     1381 cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg
     1441 agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca gatgcagaat
     1501 ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc
     1561 ttcgtctatt tccccggtat tttcctcctg taaccaccgc aatctttctg ctaataacac
     1621 cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa
     1681 ttcatcaatc tcctccatcg agcaataatt taattgctga atgaatcgct gccaggcgga
     1741 gggcagaaaa cgttcactat cagaggcaag taacgcacga aaggcgagca gaatttcatg
     1801 ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg
     1861 tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg
     1921 aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc
     1981 tatatcgagg atcttccagc aatttttcat aggtcgctca tatccttaag cgtttccggg
     2041 catgcagaat agtaagccaa tggttgccag gacaaggtag ggtaatacgc atcatcactg
     2101 gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat
     2161 tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg accaaaggta
     2221 taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag atgaatgcgc
     2281 gactggatcc atagactttc ttcttcgctc aaccagttat taagtacgcc cattcggctt
     2341 aaaaaaccca tccggacata atcccaggct ttgattcccc cttcgccaca gcattcggcg
     2401 gtgctggcga caaattgcgc ataagcctga ccttgctcat ttagttcagc aagatagtca
     2461 cgccattcac atggcgaata acgaaaccag cggtgataaa accctgccaa ccgagcggca
     2521 tgaccgttat cgatcattcg gtgaattgtg cgcagcaggt catcacggtc atcaattccc
     2581 catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttgggtga tgaatgcgaa
     2641 ggtaaacagc attcattgcg tattcccatc ggagtggaaa gtgcaaacaa tattttttgc
     2701 gactccatgt ccatataatt t
//
LOCUS       DAJOSY010000232         2676 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJOSY010000232 DAJOSY010000000
VERSION     DAJOSY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.234"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..86
                     /locus_tag="OHN27_004403"
     CDS             <1..86
                     /locus_tag="OHN27_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HCQ2288713.1"
                     /translation="IGALLAAGPEMMSRSQKQLGISSISIG"
     gene            150..338
                     /gene="traS"
                     /locus_tag="OHN27_004404"
     CDS             150..338
                     /gene="traS"
                     /locus_tag="OHN27_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HCQ2288714.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            322..1122
                     /gene="traT"
                     /locus_tag="OHN27_004405"
     CDS             322..1122
                     /gene="traT"
                     /locus_tag="OHN27_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HCQ2288715.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            1212..>2676
                     /gene="traU"
                     /locus_tag="OHN27_004406"
     CDS             1212..>2676
                     /gene="traU"
                     /locus_tag="OHN27_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HCQ2288716.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMALAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAIEVGL"
BASE COUNT          682 a          709 c          672 g          613 t
ORIGIN      
        1 tcattggggc gctacttgcc gccggacctg aaatgatgag tcgcagccag aaacagttgg
       61 ggatttcctc aatttccatt ggctaatcct gtccagggca ctggcattta tccggtgccc
      121 ttcctgttcg gcaatacaag gaggcacata tgtctgcctg gagcccggag cacataacgt
      181 tacgacgaac agaaaacggc aatgtgattg cccacgacag tcgggacgat caggaattca
      241 tttttgcaga atgctgttcc gatgaaatcg caaacatcat tctggaagcc gtgaaacgtt
      301 acaccagtac ggagcctgct catgcaacac agcattaaag acttgtggtt gtatcccttt
      361 ccagaaattg atgtggttca tacacaggag ccactgttac cggaaccgga attaacaacg
      421 cccggtcgat gtatctgctg ccggcaaaac gtcaggcacc gtttcagact ggatgactca
      481 tggccactca gacagcttac cgacacaatc agtgacacac gagttcgtct taataaggcc
      541 acagaacatc tggataagtt aaaaaagcgt ggtgaaccag tcgcaaccgg cgaaaaggaa
      601 aagtacaaca cggcagtaaa agccgctgaa cgggcgcttg agcaggcccg tctgtctgcc
      661 agacgtcttt cactccgcca tgtacagaaa gctgaaatca ccagtacaga gtcgttgtca
      721 gagaaagagc aggaactgtt tcatgaagac ggcccgcctt acagcttatg tgctttctgc
      781 cacgcctggc attcactgaa tggttatgcc gcagcacagg gcgttatggt gtggctaccc
      841 gaccttcatc catccacggt cgtggccctt aaccgtcgtt cattacagga agttttcagt
      901 aatgataagt tccgggtccg gcggggacgg gaagcgctct ctgcactgat gcaaaacaga
      961 ctggcggttg aggataaatt ccgcagtttc cgcccggccg atttcgctga cgtttttcgt
     1021 cgctatccac cttccggtcg ttcgcccctg cgtgaaaaaa tgaacgggat tgccctgatt
     1081 ctgacaccag attcattcat caaaaaagaa tacgttgatt gatttttctt catgccactc
     1141 cagcgcgggg tggcattaaa aaaatcaaac tgcacaaacc tccctcctgt aagtccggag
     1201 ttccctcacc tatgaacata aaccgtttaa tcttcactat cgaagactgt ctgaacacgc
     1261 tatcccgttt ctctgtcgca agcagttttg ttgagtattg cgatttacgt actgtaatag
     1321 gtctggacag acaggatcgt gaacgtcgcc catggctgaa ttcgccctat attgcagcca
     1381 caaagcgtgg agaatatctc tctgtctttg aagtcagcgg tgctttcagg gaaatggatg
     1441 aagcatccga ccagactggc ccaggttcac tggaatcgct gatcaccagc atgagtgaca
     1501 gcctgaatac ggcatataaa aacagcggtc ataaaatcag cttcgttttt gagcgtgatc
     1561 ctgacatggg gaaagaagaa atcgaagaca tggtcgcccc acaaaagcgc tcactggcta
     1621 atacaggtat ccagttacag gatgtggtcg atgagaaagt cacaacactg tccccatggc
     1681 tggtccggga gcgttgctgg ctggcgatct ggagtggacc cgaccttatc agcaacagcg
     1741 atcgtaccgc ccatgatgag cttgtcagaa gactggctga acgggtaccg aaagcacgct
     1801 ttgcccagtc cccctggcag tggaccttat ctgcactgaa aatccgccac gaagcttttc
     1861 ttgataatgt ggaacaggca ctgagacatt catctgatgg cctgattctc cggctactgg
     1921 atatacacga ggtcgggcgt gaaatacgtc gtcagacaga acggcacagc acaccgcgta
     1981 actggcaacc ccacctgcct gaagatgccc agccagcggg atatcgctgg actgacgatg
     2041 aatccgtact acatgctcct tcattgcatt tgcagttatt caacactcag gtgaccacac
     2101 agggcaacct ggttcaggcg ggcggactct ggcacggaat ggtatcgatt actttacctc
     2161 cgcaaaatct gcagacgttt aacgaactgg tccgtgccgt cccacgcgca gtcccctggc
     2221 gcatcagaat ggatctgatg cccggcggga tgaaggctct taatctgaag aaaacgcttc
     2281 tgacttacag cagtttcatt tctgccgttc gtccaatgta tgaatccgtt atggccctgg
     2341 cggcaacgga tgaaaaagaa cctgtctgca taatgaccat catggcctcc acgtggggaa
     2401 aaacacgtga aatatgcaca cgaaaccagg ccattctgaa atcagccatt gaaggctggg
     2461 gcgtatgtgg caccacgaca acatttggcg atccccgacg cgcctgggtg aataccattc
     2521 tcgcggcatc cggaggctcc ggcccggttc ccctgtatcc cccgttgtca catgccatct
     2581 cgcttttccc gcttaacagg gcaggctctg tctggcgagg caaagggaat ctgatgttac
     2641 atacagaaga cggttccgca attgaggttg gtctgg
//
LOCUS       DAJOSY010000233         2670 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJOSY010000233 DAJOSY010000000
VERSION     DAJOSY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.235"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..374
                     /locus_tag="OHN27_004407"
     CDS             <1..374
                     /locus_tag="OHN27_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704361.1"
                     /note="Wzb shows phosphatase activity towards the
                     autophosphorylated Wzc protein, which induces colanic acid
                     biosynthesis; catalyzes the phosphorylation of UDP-glucose
                     dehydrogenase, an enzyme involved in colanic acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HCQ2288717.1"
                     /translation="ILRSKITNRKIDSAGTGALVGFPADHSAIKVSEKNGISLEGHKA
                     TQFSSKLGRQYDLLLVMEKNHIEQISNIAPEARGKTMLLGHWIGMKEIPDPYRKSDEA
                     FESVYQLIEESCQCWADKLGR"
     gene            392..2545
                     /locus_tag="OHN27_004408"
     CDS             392..2545
                     /locus_tag="OHN27_004408"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCQ2288718.1"
                     /translation="MTSVINNKMAPQDGNEIDFGRLVGEVIDHRKLIVAITTGFTVIA
                     VLYSLLATPIYQANALIQVEQKQGNAILSSLSQILPDGQPQSAPEISLLQSRMILGKT
                     VDELALQAQITPKYFPLIGKGLARIMGKKPGEIALSQINIANVNSNEATKFILTVKDE
                     KNYTIEGDGFKLNGTVGTPLYGQGISLLVSKIDAEKGSQFEIEYISKLKAITLLQDSL
                     SVVDQGKDTGMLSITFTDENPALAERIIDSISQNYLTQNIARQAAQDAKSLEFLNQQL
                     PIVRNDLDNAEDKLNAYRKQRDSVDLTMEAKTVLDQIVNVDNQLNELTFREAEISQLY
                     TKEHPTYKALMEKRQTLQEEKNKLSKRVSSMPATQQEVLRLSRDVESGRAVYLQLLNR
                     QQELNIAKSSAIGNVRIIDQAVTIPKPVKPKKIIIIAVGFILGLLCSIGLIILRVFLR
                     RGIESPEELEEMGINVYASIPVSEWLMKRTTKANKNQSDTLLAVENPADLAIEAIRGL
                     RTSLHFAMMEAKNNVLMISGASPNAGKTFLSTNLAAIISSGDKKVLFIDADMRKGHVH
                     KMLGSKNEKGLSELLSGLVTIENVVEKVTLGGFDYIGRGQVPPNPAELLMHPRFENLI
                     EWSAKHYDLVIVDTPPILAVTDAAIIGKYAGTTLLVARFETNTAKEIYVSTKRFEQSG
                     VMVKGCILNGVVRKASSYYGYGYNHYGYSYDDIKK"
BASE COUNT          950 a          416 c          544 g          760 t
ORIGIN      
        1 gaattttgcg aagtaagatt accaatagaa agatagattc agcaggaaca ggagctttag
       61 ttggttttcc tgctgatcat tccgcaataa aagtatcgga aaaaaatggt atttccttag
      121 agggccataa agcgacccaa ttctcatcta agctagggag acaatatgat ttattacttg
      181 tcatggaaaa aaatcatata gaacaaataa gtaatattgc accagaagca cgtggaaaaa
      241 caatgcttct aggtcattgg ataggtatga aggagatacc agatccatac aggaaaagcg
      301 atgaggcttt tgaatctgta tatcaattaa ttgaagaaag ttgtcagtgc tgggctgata
      361 aactgggtag ataaattcag gaaattatcg tatgacatca gtgataaata ataaaatggc
      421 tccgcaagat ggcaatgaaa tagattttgg tcgattagtt ggagaagtca ttgaccatcg
      481 aaaattgata gtagcaataa caacaggctt tacagtaatt gcagtcctct attcattact
      541 tgcaactcct atctatcaag caaatgcttt aattcaagta gaacagaaac aagggaatgc
      601 aattttaagt agtttaagcc aaatacttcc tgatgggcaa ccacaatctg caccagaaat
      661 atcgctatta caatcaagaa tgatattagg gaagaccgtt gatgagctag cgctacaagc
      721 tcaaataact cctaaatatt tcccattgat tgggaaaggc ttagctagga ttatgggtaa
      781 aaaaccagga gagatcgctt tatcccagat aaatatagcc aatgtaaatt cgaatgaagc
      841 tacaaaattc attctaacgg ttaaagacga gaaaaattat acaattgagg gcgatggttt
      901 taaattaaat ggcactgtcg ggactccgtt atatggtcag ggtatatcat tattagtgag
      961 taaaatagat gccgaaaagg gaagtcaatt tgaaatagag tatattagca agttgaaagc
     1021 aattaccttg cttcaggatt ccttaagtgt tgttgaccaa ggcaaagata cgggaatgct
     1081 aagtattact tttaccgatg aaaatccagc cttagcagaa cgaatcatcg atagcataag
     1141 tcaaaattat ttaacacaaa atatagctag acaagccgca caagatgcta aaagtctcga
     1201 gtttttgaat caacaactac caattgtacg taatgattta gataacgctg aagataaatt
     1261 aaatgcatac cgtaagcaaa gagattctgt tgatttaaca atggaagcaa aaacagtact
     1321 agatcagata gtaaatgttg ataaccaatt aaatgaatta acttttcgcg aggccgagat
     1381 ttctcaatta tacactaaag aacatcctac ctataaggct ttgatggaaa aaagacaaac
     1441 tttacaagaa gaaaaaaata agcttagcaa acgagtaagc agtatgccag ctacccaaca
     1501 ggaagtttta cgtctcagcc gagatgttga gtctggtaga gctgtttatc ttcagttact
     1561 taatcgtcag caagaattaa atatcgctaa atcgagcgcg ataggaaatg ttcggataat
     1621 tgatcaggct gtaactatac ctaaaccagt taagcctaag aaaatcatta ttattgctgt
     1681 cggatttatc cttggcctct tatgctcaat tggattaatt attttacgcg tttttcttcg
     1741 tagaggtatt gagtcgccag aagagttgga agagatggga attaatgttt atgcaagtat
     1801 tcctgtctcc gagtggttaa tgaaaagaac aacaaaagcc aacaaaaatc aatcagatac
     1861 tttgttggct gtagaaaatc cggctgatct tgctattgag gctattcgtg gtttaagaac
     1921 tagtttgcat tttgcgatga tggaagcaaa gaataatgtc ttaatgatat caggtgcgag
     1981 tccaaatgca ggtaagactt ttttaagcac aaacttagcg gcaattattt catctggtga
     2041 taaaaaggta ttgttcatcg atgctgatat gcgtaaaggt catgttcata aaatgctcgg
     2101 tagtaaaaat gaaaaaggac tatctgagtt attatcaggc ttagtaacga ttgagaatgt
     2161 tgttgagaaa gtaactctag gcggttttga ttatataggc cgagggcaag taccaccaaa
     2221 ccctgcggag ctactgatgc atcctagatt tgaaaactta atcgaatggt ctgctaaaca
     2281 ctatgactta gtaattgttg acacaccacc aatccttgca gtaactgatg cagcaattat
     2341 aggtaagtat gcgggtacta ccttgttagt tgctagattc gagacaaaca ctgcaaaaga
     2401 aatatatgtt agtaccaaac gttttgaaca aagtggagtc atggtgaaag gatgtatttt
     2461 aaatggtgtc gttagaaaag ctagtagcta ttacggatat ggatataatc attacggtta
     2521 ttcctatgac gacataaaaa aatagaatat tcgctacttt gattcttgat ctttcaaatg
     2581 gaagagggag gggactctcg ctcttagtgt aaaaaagttt tgttatatat aattcttaag
     2641 cactttaact taaaattgta aaatagcaga
//
LOCUS       DAJOSY010000234         2663 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJOSY010000234 DAJOSY010000000
VERSION     DAJOSY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.236"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1713
                     /locus_tag="OHN27_004409"
     CDS             <1..1713
                     /locus_tag="OHN27_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCQ2288719.1"
                     /translation="RYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQ
                     TRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIAR
                     DAHYLYRYDRYGRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVE
                     SRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVSWYGWDGDRLTTIQNDRTRIQTI
                     YQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESE
                     ILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGA
                     TEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQD
                     PIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAK
                     QLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTK
                     TASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSR
                     GIRK"
     gene            1710..2279
                     /locus_tag="OHN27_004410"
     CDS             1710..2279
                     /locus_tag="OHN27_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288720.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
     gene            2505..>2663
                     /locus_tag="OHN27_004411"
     CDS             2505..>2663
                     /locus_tag="OHN27_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCQ2288721.1"
                     /translation="MELKKLMEHISITPDYRQAWKVVHKLSDILLLTICAVISGAEGW
                     EDIEDFGET"
BASE COUNT          778 a          627 c          662 g          596 t
ORIGIN      
        1 cgttatgaac tcaccaccgc ttatacccct gccgggcagt tacagagcca gcacctgaac
       61 agcctgctgt ctgaccgcga ttacacctgg aacgacaacg gcgaactcat ccgcatcagc
      121 agcccgcgcc agacccggag ttacagctac agcaccaccg gcaggctgac cggcgttcac
      181 accaccgcag cgaatctgga tatccgcatc ccgtatgcca cagacccggc aggtaaccgc
      241 ctgcccgacc cggagctgca cccggacagc accctcagca tgtggccgga taaccgtatc
      301 gcccgtgacg cgcactatct ttaccggtat gaccgttacg gcaggctgac agagaaaacc
      361 gacctcatcc cggaaggggt tatccgcacg gatgatgagc gcacccaccg gtaccattac
      421 gacagtcagc accggctggt gcactacacg cggacacaat atgcagagcc gctggtcgaa
      481 agccgctatc tttacgaccc gctgggccgc agggtggcaa aacgggtgtg gcgacgtgaa
      541 cgggacctga cgggctggat gtcgctgtca cggaaaccgc aagtgagctg gtacggctgg
      601 gacggcgacc gcctgaccac aatacagaac gacagaaccc gcatccagac gatttatcag
      661 ccggggagct tcacgccact catcagggtt gaaaccgcca ccggtgagca ggcgaaaacg
      721 cagcgccgca gcctggcgga tacccttcag cagtccggcg gcgaagacgg tggcagtgtg
      781 gtgttcccgc cggtgctggt gcagatgctc gaccggctgg aaagtgaaat cctggctgac
      841 cgggtgagtg aggaaagccg ccgctggctg gcatcgtgcg gcctgacggt ggagcagatg
      901 caaaaccaga tggacccggt gtacacgccg gcgcgaaaaa tccacctgta ccactgcgac
      961 catcgcggcc tgccgctggc gcttgtcagc acggaagggg caacagaatg gtgcgcagaa
     1021 tacgatgaat ggggcaacct gctgaatgaa gagaacccgc atcagctgca gcagcttatc
     1081 cgcctgccgg ggcagcagta tgatgaggag tccggcctgt attacaaccg ccaccgctat
     1141 tatgacccgc tgcaggggag gtatatcact caggatccga ttgggctgaa ggggggatgg
     1201 aatttttatc agtatccgct gaatccggtt cagtatatag attcaatggg actggcatca
     1261 aaatatggac acttaaataa tggcggatat ggagcgagac ccaacaaacc gcctacgccc
     1321 gatccaagta aattgccgga catagcgaaa caattaagac tgccatatcc tattgaccag
     1381 gccagtagtg cgcctaatgt tttcaaaaca ttcttcagag cattaagccc ttacgactac
     1441 acactgtatt gcaggaagtg ggtaaaacca aatctgactt gtacgccaca ggatgattcc
     1501 cagtatccag ggatggatac aaagacagca agtgattacc tgccacagac aaattggcca
     1561 acaactcaat taccaccagg atatacttgt gcagaaccct atttattccc agacattaat
     1621 aaacccgatg ggccagcaac agcagggata gatgatttgg gtgaaatttt agctaagatg
     1681 aaacagagaa catcgagagg aataagaaaa tgaaaagagt tttgttcttt ttgctgatga
     1741 tatttgttag ttttggtgtt atagctgatt gcgaaataca agctaaagat catgattgtt
     1801 ttactatttt cgctaagggt actatttttt ccgcgtttcc tgtcttaaat aataaagcca
     1861 tgtggagatg gtatcagaat gaggacatcg gtgagtatta ttggcaaaca gaactgggta
     1921 catgtaaaaa taataaattc acaccaagcg gggcgagact cttaattcgc gtcgggtcac
     1981 tacgccttaa tgaaaatcat gccatcaaag gaacgttgca ggagttgata aatacagcag
     2041 aaaaaacggc gtttctcggc gatcggttta gaagttatat aagagcgggt atctatcaaa
     2101 aaaagagcag tgatccagtt cagttgttgg cagtccttga taattcaatc atggtaaaat
     2161 attttaaaga tgagaaacca acttatgctc gaatgacagc acatctcccg aacaaaaatg
     2221 agtcttacga atgtttaata aagatacagc atgaactaat tcgtagtgaa gagaaataaa
     2281 atctcttcct gagaaaacaa attaatcatt gtgaaaaatt atataactca tggaataggt
     2341 gtttattttg cgttccattt gcagggaaag atcacgtaac gctacttttt tgtactaaat
     2401 aattcgaatt ttaagttcaa caattgagat actacttatt gtctaaagct gtttttcata
     2461 gcttatacat gatcaaatac tccttacata aataaggtga acaaatggaa cttaaaaaat
     2521 tgatggaaca tatttctatt acacccgatt acagacaagc ctggaaagtg gtgcataaat
     2581 tgtcagatat tctactgttg actatttgtg ccgttatttc tggtgcagaa ggttgggaag
     2641 atatagagga tttcggggaa aca
//
LOCUS       DAJOSY010000235         2593 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJOSY010000235 DAJOSY010000000
VERSION     DAJOSY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.237"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            208..1743
                     /gene="istA"
                     /locus_tag="OHN27_004412"
     CDS             208..1743
                     /gene="istA"
                     /locus_tag="OHN27_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCQ2288722.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTFASELVMPDMLYLDTEMRKPGVTRQLLWMEYKAQA
                     GNKAMGYSHFCRCYREWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRILDAPALSPLPLAPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKAGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRQHGTWTPERLIEQGKRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PERLEKACYVALHYNAPDRRFIDNLLRHHRENLELPLSRQGEQHPAYASEHENLRGPG
                     YYH"
     gene            1760..2515
                     /gene="istB"
                     /locus_tag="OHN27_004413"
     CDS             1760..2515
                     /gene="istB"
                     /locus_tag="OHN27_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HCQ2288723.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNAGLKYQATPEGLRYPASRGLRAEQMRELLNGHYITHRKNL
                     LITGPTGCGKSWIANALGEQACRQKHSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQK
                     IQVLILDDLGLEQLSNAQCNDLLELIEDRYGQSSTIVVSQFPVDKWHGLMENPTTADA
                     ILDRLVHNSHRVVLQGESLRKNPPVVESSEKTR"
BASE COUNT          699 a          685 c          683 g          526 t
ORIGIN      
        1 cagtttggca ttcgaatcga tcatgatttg gcatccatct ccgatcacag tttggcatca
       61 caatcgatca cgatttggca tgcttccgat cattgattgg catcctgcca gtcactccgg
      121 gaattaactc ttttcaccac agtctccatt gccgtgttta aaccagtgga gacgtcaatg
      181 tccagaaaga gaaaatccag gagcactatg gaaacctgtt ttaagattct tcagctcaag
      241 ttcgacaaga agctgactaa ccgttgtatc ggactgacgt tacacatcag cgcctcaacc
      301 gtttttgagg ttcttgcccg cttcaaagcc tcttctctgt catggccatt acctgctgat
      361 atatcccatg acacgctgga gaaactcatt tttccgccca aagacacctt tgcatcagag
      421 cttgtaatgc ctgacatgtt gtacctcgat acggaaatga gaaaaccagg tgtgacgcgg
      481 caactgttgt ggatggaata caaggcacag gcgggtaaca aagcgatggg atactcgcac
      541 ttctgccgtt gctaccggga gtggaaaaaa acgcgacgtc tctccatgcg ccaggagcat
      601 cgtgccggcg aaaagctttt tatcgacttc tgtggtccta cggttcctgt tattaatccg
      661 gacaccggtg agatacgccg cgtggcaatc ttcgtggccg taatgggggc gtccaactat
      721 acctacgttg aagcgtgcga aggtcaggac atgatgtcat ggttaaatgc ccacagccgt
      781 tgcctgacct tcctgggcgg cgtaccgaag ctgctcatcc ccgataacct cagaagcgca
      841 gtgaaaaagg ccgatcgata tgagcctgtc atcaacgaca gttatcaggc actggctgag
      901 cactatggca cggttatcat cccggcccga ccacgcaaac ctaaagataa accaaaagca
      961 gaaaacggcg tcctcatcgt cgaacgatgg cttcttgccc gcatccgtaa tgaaaccttc
     1021 cacacgctca gggcgctgaa tgcgcgcctt agggagttac tgacagacat gaataaccgg
     1081 ccaatgaagg ggtacggcaa tcaaacccgt gcagaacgct tccgcatact ggatgcgcca
     1141 gcactctctc cgctgcctct ggcaccttat gaatacacgg aatacaaagc tgtgaaggtt
     1201 ggccctgact accacgttga atatgcccgt cactggtact ccgttccaca cgaactggtc
     1261 ggtcagcgtc tgtcactgaa ggcggggcag tcggtggtac agctctggca caaggggcag
     1321 tgtgtggcgc aacatccgcg cagcacacat gaatataaac acacaaccaa tccgcttcac
     1381 atgccagagc gccaccggca gcacgggaca tggacaccgg aacgcctgat cgagcaggga
     1441 aaacgtacgg gcccgtccac aggccgggtg gttgaatcca tgctgaaggc aaaaccccat
     1501 ccggaactgg cttatcgtgc agttctgggg ctgctggcac ttcagaaaaa atacggcccg
     1561 gaacggctgg aaaaggcctg ttacgttgcc ctgcactata acgcccctga cagacgcttt
     1621 atcgacaatc tgctgcgtca ccatcgggag aacctggagc ttccattatc ccgccagggc
     1681 gaacaacatc cggcatatgc ttcagagcat gaaaacctgc gtggtccggg ctactaccac
     1741 tgacagaagg aacaacagta tgagtgataa cctgttaaac aaactgaccc aactgaaact
     1801 cccggcgatg gccgggagcc tgatacggca gcgggaaacg cctcagacct acgacgaact
     1861 gtcgttcgag gaaaggctga cgctgctggt tgatgacgag cttctgagca gggaaaacag
     1921 ccgtgtggca cggttgagaa aaaatgccgg cctgaaatac caggcaacac cggaagggct
     1981 gcgctatccg gcctcaagag gtctgcgggc tgaacagatg agagaactgc tgaacggcca
     2041 ctacataacc cacaggaaaa acctgctgat aaccggtccg acaggatgtg gcaagagctg
     2101 gatagccaat gcgctgggcg aacaggcatg tcgacagaaa cacagcgtcc ggtactgtag
     2161 aaccgggcga ttactggaac aactggctca gggacgtgtt gatggcagct ggctgaaata
     2221 tctgaaacag ttgcaaaaaa tacaggttct aatcctggac gatctggggc tggaacagtt
     2281 aagtaacgcc cagtgtaatg accttctgga gctcatagag gatcgttacg gacaaagcag
     2341 cacgatagtg gtaagccagt tcccggtaga taaatggcat ggtctgatgg agaacccaac
     2401 aacagcagat gccatcctgg acagactggt acataactca caccgggtgg tccttcaggg
     2461 agagtcactg aggaaaaatc ccccggtagt ggaaagcagc gaaaaaacgc gttaaaaagc
     2521 ggtagaaaaa gttaagttcc cggttgatcg aatggatgcg aatcgctgat cggacttcga
     2581 tgccaatccg tga
//
LOCUS       DAJOSY010000236         2590 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJOSY010000236 DAJOSY010000000
VERSION     DAJOSY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.238"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..138
                     /locus_tag="OHN27_004414"
     CDS             <1..138
                     /locus_tag="OHN27_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCQ2288724.1"
                     /translation="QLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEKTINQANA
                     R"
     gene            167..751
                     /gene="traX"
                     /locus_tag="OHN27_004415"
     CDS             167..751
                     /gene="traX"
                     /locus_tag="OHN27_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HCQ2288725.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            848..>2590
                     /locus_tag="OHN27_004416"
     CDS             848..>2590
                     /locus_tag="OHN27_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HCQ2288726.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTQTAARGIFEMELCKYAVNAGLNDFNQTAKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPTDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYK
                     TQLQNTTYTPPLGTVTSANEQRMANAQDPKSALSEMMSFFVYRTNWIATEISGTGTTS
                     DQVNPLIKMKNIGDYTLGTTEILWSGYTTVRVLATVGDNSIFGKVANLFSGLPKGLVA
                     LLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAY"
BASE COUNT          710 a          622 c          605 g          653 t
ORIGIN      
        1 caactgaacg acctgaaaga acagatccgc cagggtaatg tcatctctgg acagcaactg
       61 gctctgaccg cccgccagta ttatgaaaaa caactcggta gccttgagaa aacaatcaat
      121 caggcaaacg cacgttgaaa gctgacgtca gaacaggact gaaaaaatga ataagttacc
      181 ggaaaattca gaccagacga aaaacgtcaa aaaatcacca tccataaccc gcatagccac
      241 ccgggggatt attaacattt ttgttcctct gatggagact cgcctgataa cgaattctgt
      301 acagcatatg ctggaacaac aaaaacgcag actggacaga tttcgtcagg taaataataa
      361 aaaggcgcaa ctccacctca cgtgggaaga agctctgcag gccagccata tgtcagtaga
      421 cgatctggat cgtcgtttcc gccggcgcag aacagtatgg cgattctgtt gctggagtct
      481 gctggccatc gctttgtttc tcagtggtat gctctttgcc gcctcttcat tacctcttac
      541 gactctggta cgcgccatca gcactcttgt gctgatactg tcaggcgtcg ctctttgtgc
      601 ttccagagca ctgattgtta cataccgcct ctggcagttg catgaacgaa aagtcagcga
      661 acccgagcag gggactttcc gtgattttct gaacgaccgc aatggctggc gcaatgccac
      721 gttaattgcc gtaaccagta aacaatactg atcacgtcca gatgccagta ccactggcat
      781 gtcgccatcc cccttaatta aatcctgtgt ggcctttgcc catacgcaat tctattccgg
      841 agttactgtg aaaattttac ttcgcgcact ctgtgcaggc cttgctatat cctcactgcc
      901 ggcaatggct tcagtgacct atcaggatat tgtgtctgcc gcaaccaatc ctgacgatct
      961 gtctcgccag gcgctggtca cgatattcgg tgatgtagtc accaatccac tgtcaaccag
     1021 cgcccccact ctgatcggaa gcatgttcgg ggcgttcaac agtatcatcg ccgtacttgc
     1081 cgttgtctgg tttatgttca ttggtatccg tcatgttgtg cgttcggggc atcagggaca
     1141 ggtattcagc accggacgcg atatcgtcgg cacactgagt gtcgttgcag gttttctgat
     1201 gatcgttcca accggaaatg gctggagtat cgctcagctc attatgttat ggggcgcatc
     1261 gataatgggg gttgggtccg ccaatgtgat ggtgcaactt gccgctgata atatcgcaaa
     1321 tggttattca atgactgtac agccagttca ggcatccaca caaacagcag cgcgtggcat
     1381 ttttgaaatg gaactgtgta agtatgcggt taacgcagga ctaaatgatt ttaatcagac
     1441 ggccaaatcc tcgacttcac tgatgacaga atcagctaaa accgccagtg gtaattacac
     1501 tgtcacggta tccaatggca gcggtatctg tggttcagcc agcctgtcag tagaaggaaa
     1561 cggaacaaca gatcaatcga cgattggaaa attctttaat cccttcagta aaaacgaata
     1621 cagcggtgtt atctcagcac aacgtgcagc catggataat atgattagcg acatggacaa
     1681 cgctgccagt gagtttgtga caacatttct ggaaaaaaga aattctggca acggaacatt
     1741 gccggatatc gagacacgga ttcagagagc agccgacgag tatgaacgtg ctgtacaaaa
     1801 atcgctaccc actgataacg gagagcaatc cagaaaagag gctcttaaat cttatctcac
     1861 aacctacggg tgggtaacgc ttggcgcatg gtatcagacc tttgccactg ctaaccagcg
     1921 tctggccgag ctggctgaca gagctccagc tgtaacttca atgtcatcac tgggagaagt
     1981 tggtgatact gacttattca gtgcagtcat gagtgcctac aagactcaat tacagaacac
     2041 cacctataca ccacctctgg gcactgttac atcagccaat gagcaacgaa tggccaatgc
     2101 gcaagatcct aagtctgcct tatctgaaat gatgagcttc tttgtctatc gaaccaactg
     2161 gatagcaaca gagataagtg gcacaggaac aacaagcgat caggtcaatc cattgataaa
     2221 gatgaaaaat attggtgatt atacattagg aacaacagag attctctgga gtggatatac
     2281 aacagttaga gttctcgcga cagtaggtga taactctatt ttcggaaagg ttgccaattt
     2341 attctctgga ttacctaaag gattagttgc tctgctggat gctgcagctc ccccaatata
     2401 tttcttgttg ttcctgttgt tttgtgcggg tttttcctta tcaatttacc taccatttat
     2461 accattcatt ttctggatga caggcattgg gaactggata gtcagtgtat taataggatg
     2521 taccgccgga cctctgtggg ctgcaacaca tctcggaaca tcagaagata gaggaagtcg
     2581 ggcagcctac
//
LOCUS       DAJOSY010000237         2556 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJOSY010000237 DAJOSY010000000
VERSION     DAJOSY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.239"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(114..320)
                     /locus_tag="OHN27_004417"
     CDS             complement(114..320)
                     /locus_tag="OHN27_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCQ2288727.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            633..722
                     /gene="kdpF"
                     /locus_tag="OHN27_004418"
     CDS             633..722
                     /gene="kdpF"
                     /locus_tag="OHN27_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCQ2288728.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            722..2395
                     /gene="kdpA"
                     /locus_tag="OHN27_004419"
     CDS             722..2395
                     /gene="kdpA"
                     /locus_tag="OHN27_004419"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCQ2288729.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGTDSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQAASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2418..>2556
                     /locus_tag="OHN27_004420"
     CDS             2418..>2556
                     /locus_tag="OHN27_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288730.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LT"
BASE COUNT          527 a          623 c          689 g          717 t
ORIGIN      
        1 atatatgttt cgattggtat aacgatgtct aaatattcct ctgaacatct tcctgataca
       61 aaaaaccgcc agcgtttccg ctggcggttt tcaatgtatt caaaactcag caatcaataa
      121 aaatcaccag ttgccttttc ggcctgatcc atccacaccg gtttatcgct ggttttcgac
      181 cagacgcgat gcaggtagct gtaaaagcgg gcgcggtctt tccagaaaag catgaaaggc
      241 agcgccagaa cgcccgctgc aaccgcataa gtacggcgta aaaagataag ccatgcagga
      301 tattctctgt agagttccat acttttctcc cccttttgta tctacccggt gaatggcacc
      361 ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc ttttgtgtaa ttttactact
      421 catccgacca cttatttttg cttattgatg gtttatttac attcatcctg taattaagtt
      481 acacaaaagt taaattaata ctaaacatta gttaaatcat ggcttttgcc atttttatac
      541 tttttttaca ccccgcccgc agatttttgc gaaatctttg cagccagaat tctacccttc
      601 cggtatcact tttaggccac tggaggtgca ctgtgagtgc aggcgtgata accggcgtat
      661 tgctggtgtt tttattactg ggttatctgg tttatgccct gatcaatgcg gaggcgttct
      721 gatggctgcg caagggttct tactgatcgc cacgttttta ctggtgttaa tggtgctggc
      781 gcgtccttta ggcagcgggc tggcgcggct gattaatgac attcctcttc ccggtacaac
      841 gggcgttgag cgcgtacttt ttcgcgcact tggcgtctct gaccgtgaga tgaactggaa
      901 gcaatatctt tgtgccattc tcggcctgaa catgctgggg ctggcggtgc tgttttttat
      961 gttgctcggt cagcactatc tgccgcttaa tccacagcag ttgccagggc tgtcgtggga
     1021 tctggcgctg aataccgccg tcagctttgt caccaatacc aactggcaat cttatagcgg
     1081 tgaaaccacg ttgagctatt tcagccagat ggcgggctta acggtgcaaa actttctttc
     1141 tgccgccagc gggattgcgg tgatttttgc cctcatccgt gcgtttaccc gccagagcat
     1201 gagcacgctc gggaatgcct gggtcgatct gctacgcatc acgttatggg tgctagtccc
     1261 tgtggcgttg ttgattgcac tgttttttat tcaacaaggt gcgctgcaaa actttctgcc
     1321 ttatcaggct gtgaataccg ttgaaggagc gcaacagctg ttacccatgg ggcctgtagc
     1381 ttctcaggaa gcgatcaaga tgctcggtac taacggcggt ggcttcttta atgccaactc
     1441 gtcgcatccg tttgaaaacc caaccgcact gaccaacttc gtgcagatgc tggcgatctt
     1501 cttgatccca acggcgctgt gctttgcctt tggtgaagtg acgggcgatc gccgccaggg
     1561 gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc tgcgtaggcg tggtgatgtg
     1621 ggcagaagtt cagggtaatc ctcatctgct ggcactgggc acggacagca gcatcaatat
     1681 ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc ctgtttgcgg tcgtgacgac
     1741 ggcggcttcc tgtggcgcgg tgattgcgat gcatgattcg tttaccgctc tcggtggcat
     1801 ggtgccgatg tggctgatgc aaattggtga agtggtgttc ggcggtgtcg gttctggtct
     1861 ttacggcatg atgctgtttg tcctgctggc ggtgtttatt gccgggctga tgattggtcg
     1921 tacaccggaa tatctgggta aaaaaatcga cgtacgcgag atgaaactga ctgcactggc
     1981 aattctggtt accccgacgc tggtgctgat gggcgcggcg ttggcgatga tgaccgacgc
     2041 cggacgtagc gccatgctca accctggccc gcatggtttt agcgaagtgc tgtacgccgt
     2101 gtcatccgcc gctaacaaca acggcagcgc ctttgccgga ttaagcgcca actctccgtt
     2161 ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc ttcggggtga ttatcccggt
     2221 gatggcaatt gccggttcgc tggtgagtaa aaagagccaa gccgccagct ccggcacgct
     2281 gccaacgcac ggcccgctgt ttgttggcct gttaatcggc accgtgttgc tggttggcgc
     2341 actgaccttt atccctgccc tggcgcttgg tccggtggcg gaatatctct cctgatgata
     2401 ttgagtgagc actgaatatg agtcgtaaac aactggcgct attcgaacca acacttgtcg
     2461 ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca ggcgcaatgg cgcaatccgg
     2521 tgatgtttat cgtctggatc ggcagtctgc tgacca
//
LOCUS       DAJOSY010000238         2518 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJOSY010000238 DAJOSY010000000
VERSION     DAJOSY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.240"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(24..371)
                     /gene="trbA"
                     /locus_tag="OHN27_004421"
     CDS             complement(24..371)
                     /gene="trbA"
                     /locus_tag="OHN27_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HCQ2288731.1"
                     /translation="MSEDCLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMNQGAAMVVPMVIGSLPNVTSFDGFRRIFICIMLSSVWS
                     GVMWFFIRGLMTG"
     gene            complement(387..1130)
                     /gene="traF"
                     /locus_tag="OHN27_004422"
     CDS             complement(387..1130)
                     /gene="traF"
                     /locus_tag="OHN27_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HCQ2288732.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAV
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(1123..1380)
                     /gene="trbE"
                     /locus_tag="OHN27_004423"
     CDS             complement(1123..1380)
                     /gene="trbE"
                     /locus_tag="OHN27_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HCQ2288733.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTAGEML
                     AACFVIFYSGVLLFILYFCFSALTDLQKSDERKSDERNEDE"
     gene            complement(1407..>2518)
                     /gene="traN"
                     /locus_tag="OHN27_004424"
     CDS             complement(1407..>2518)
                     /gene="traN"
                     /locus_tag="OHN27_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HCQ2288734.1"
                     /translation="NAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMKL
                     RLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQNCW
                     AYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGKV
                     MVCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQAK
                     FCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGVC
                     LEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFDRLDFT
                     NFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
BASE COUNT          642 a          677 c          538 g          661 t
ORIGIN      
        1 tttttattac cgttttatat gacttagcct gtcataagcc cccttataaa aaaccacatc
       61 actccggacc atactgatga caacataata cagataaata ttcttctgaa cccgtcgaag
      121 ctcgtaacgt tcggtaatga gccaataacc attggaacaa ccattgctgc gccctgattc
      181 ataattgcac cacacagaaa ccagagactg gcagtcagca catataaaca tgcgatatta
      241 ataacaagga aaaccagtga gctcaggaga aacattttcc tgtcattacg ctcagaaaag
      301 aaataaccgg caataatgac aaatattaac agaacaacac ctgtaaacat tttcaaacaa
      361 tcttcactca taaatcaaat ccgcgattaa aaattgggtt taaaatcttc agaaacgttc
      421 aggaattgtt ttgccaggtc gtcctgcgaa ataaaaccgt atgataacgg acgaacactg
      481 ccctgtttcg ggtcaaccag catcatggcc gggaaatatt tcacgccgag gcgctgcgcc
      541 tgcccctggt cagtccggga atccggcaac agcggattaa tcacaccatc cacggaaacg
      601 ggaataacac tcagaccata ggtatccctg aagccattaa tgacctgcgc cagctgcccg
      661 tcgatggggt cctgcccccg gtaaaaaaac atgatgccgt agtgttcagc cagtttcgca
      721 atagcctgtc gctgctgcgc ctggtctgct gccagctggt tccgtaccgt gccgttgtaa
      781 tggctgtact gcaggttata gtccagttcg ggatgcgcca gcatggcctt tctggcgctc
      841 atggtgaaaa gcccggcctg ctgagtccag taattctgca gccggaaata tttcacaaaa
      901 ttatccacgc cgggatacag aatagcttcg tacagcgccc gctttgttgc cgtctgcagt
      961 gcggccagtt tctgcataat atccggctcc tgacgtggga ctgcaggtac aggtttattt
     1021 tctttttcct ttggatttat tttctcgtta taccactgcc agcctgcatc tttccctgac
     1081 gccggaaaaa taaagcagca gagtaacagt ggcagtaatg ctttattcat cttcattcct
     1141 ttcatcactt tttctttcat cagatttctg caggtcagtc agggcagaaa aacaaaaata
     1201 cagaataaac aggagcacac cggagtaaaa aataacaaaa caggcagcaa gcatctcacc
     1261 tgcagtggtt tcttttaccg tatcccacga ccagataatt accggggaaa tgacaatcgc
     1321 tgtaatcaaa agccggataa gaaaatcaat aaaacggttc gacgtgaata tgactttcat
     1381 cattttcccc ggttattttc cataccttac tgtcctgcct gtttcagctg gtcagcaatc
     1441 tgctctttca ctttctgcgt cagaacacca ctgtccggga ttttctggtt attcatcagg
     1501 tcttcgtaga agttagtgaa atccagtctg tcgaactgaa ttttctgcag ctcatcaacc
     1561 gtaatccccc gacagtcagg atgcttcgca ctgccaaaac tgatacgcag ctgtccgttg
     1621 cgcccctgct gctggactat ctgcgccagc ttcgaatcaa actgacagta actgcgtttt
     1681 ttctccaggc agacacccag cactttcttc gaacagaact ctccgacact gacggtcagt
     1741 ttgtttgatt ttgctttagc cagggctttt tcatcactgc tgcatttggc cagcccgata
     1801 tcctgtcccc agccgctgtc cttacagcag ttgctgtatc cggctgcagc cttcttacaa
     1861 aatttcgcct gaccggtgaa agcacggaca tccaccccgt tcagcgccgc aacatcctta
     1921 cctgctgcgg ccagtgccgc cagctgagaa acagcttcgg caaaatcatt actcttcccg
     1981 ctctgagcct tatcgcattc gccatcgaga cagaagacgt ctcccccaca gaccatcact
     2041 ttcccggatg tttttgactc acaggagtac gtggcatatt catgcagaca ggcaccttct
     2101 tcggagtaaa aggcgcactg gtgtgacacc agggtacagg caggattgtc ggtgtaggtc
     2161 tgacaggttc ccttgtctgc tgactgtgtg acatacgtgt cccggtatgc ccagcagttc
     2221 tggtacatgc tgtatgactg accgtctttc accagcgtcc tgttcccgcc agcctccgta
     2281 cattctgttt ttgtcagttt ccccagctcc ttgctgaacg ggcagctttc agaccagaca
     2341 acatacgggg tgtacgaggc tttttttacc ctgacaacca gacgcagctt cattgccgta
     2401 tgacgtttga attttgtatt cccgacccgg acggggtgag atgacgtaaa agcaacaccg
     2461 gcagtcagtg ttttctgtac tggtgtaaat gaggcatctg ctgagtattt ggcattcc
//
LOCUS       DAJOSY010000239         2459 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJOSY010000239 DAJOSY010000000
VERSION     DAJOSY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.241"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..126)
                     /locus_tag="OHN27_004425"
     CDS             complement(<1..126)
                     /locus_tag="OHN27_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCQ2288735.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDRNPFGAKCIFCKSMMI"
     gene            complement(137..349)
                     /gene="cspI"
                     /locus_tag="OHN27_004426"
     CDS             complement(137..349)
                     /gene="cspI"
                     /locus_tag="OHN27_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HCQ2288736.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
     gene            complement(713..1210)
                     /locus_tag="OHN27_004427"
     CDS             complement(713..1210)
                     /locus_tag="OHN27_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HCQ2288737.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRTISHDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAGVTCQRIYESLRD
                     KKYQM"
     gene            complement(1207..1740)
                     /locus_tag="OHN27_004428"
     CDS             complement(1207..1740)
                     /locus_tag="OHN27_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCQ2288738.1"
                     /translation="MNTKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPEMKLSKEKCAQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            complement(1796..2110)
                     /locus_tag="OHN27_004429"
     CDS             complement(1796..2110)
                     /locus_tag="OHN27_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCQ2288739.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDISLDH
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(2115..2330)
                     /gene="essD"
                     /locus_tag="OHN27_004430"
     CDS             complement(2115..2330)
                     /gene="essD"
                     /locus_tag="OHN27_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCQ2288740.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT          702 a          601 c          468 g          688 t
ORIGIN      
        1 aatcatcatt gatttacaaa agatacattt tgccccgaaa ggatttctgt cagaaacatc
       61 aaaagatgat gttctatact gagaaccatg gcaacacggg catctaaagt gaatatggtt
      121 tgtaatattt tctacctcaa agtgccacta catgaacagc tgcagggcct ttaggtccgt
      181 tctcaatacc aaattcgact tcctgattct cagttaacgt tttgaaatcg ttgctctgaa
      241 tagctgagaa atgcacaaac acatctttgc taccatcttt cggcgtgatg aaaccaaaac
      301 ctttttcagg gttaaaccat ttcactaaac cagtcatttt gttagacatt attattacct
      361 tttgaagaag ttagcccttg ggcagaatgg tccgaaaaaa aatatcagag agaaaaacca
      421 acaaggaaat ctcaagaggt acaaataata aaattataac aatgactgct tcagataatt
      481 ttgtaacaaa ccagaacacc attaacgcat gattaaccaa ccatagcaag gattactttt
      541 gtaaagaaaa acacacgaat gaaacaatag ctttatttat taataaaacg tgtcattctg
      601 tctaatgacc ttttatctta cccttaagat ttcaaggatt ttgactcatg gaagagtcct
      661 ttttatttaa atttcacatt cagcgctaaa aataatccga tttaatatta atctacatct
      721 gatatttttt atctcttaaa gattcataaa tccgttgaca agtcactcct gcgatgtagc
      781 gtttgtcagc aatttcagca taaagctgag cttctgctgc aatatctccg agcatgttgg
      841 tgagcattcc ttcggcggct ttggttgttt tgcctctgac ggcagcggca agatttgcgg
      901 tatgctgcgc tgcgtcaagg cgtatggcat attttgttgc ttcggcacgc agctggttaa
      961 cactatcaga cagatcagca gccctggcag aaatttcagc ggctttctgt tgtgcatctt
     1021 taacagcctc atcatgggat atagttcgcc cctgttcaat tattcgagca gcaaattgga
     1081 catttacctc ttgagataat tcggcagcat cacgctccgc ccattttttt tgccatcctc
     1141 tgtcgctcca gacattacct acgacaaatc ctaccagcac gagcaaaatc accgtgaata
     1201 tctgattcac tgttctatcc cccagcaggt taatgcactc tcctggtcac gacgaataac
     1261 ctgaccgtaa caattatttg aacgaatgcg gcaatcacgt ccaccgtcct ttatccacca
     1321 gcgaatcgcc tcgcatgcgc ctttacgatc accggcatta agccgcttat aaaacgtcga
     1381 cggaaaacac ttaccagggc caatgttata gggacagaat gacgcgatac cggctttctg
     1441 tggctcggtc agtggaactt ttatattgcg ctccacccat gccagcgcct tatcccgctc
     1501 aatggcgtta acctgggcgc atttttcctt cgacagcttc atttccggta tgacgggctt
     1561 accatccacc attgtggcac cacgacagat ggtccatata ccggaaccat cgcggtatgc
     1621 cgtagtgtgg ttaccttctt tttcatccaa aaactggtcg agaatatcag gcgcaggcgc
     1681 accgacggca atcagtgcca gaacagcagc cgacaggccg tatctgattt tagtattcat
     1741 ggatatttat caggatgcta ccaatgaaag atactggaaa gccaactgca aaaagctaac
     1801 aacccgtaat cgagttatct gaactgttaa tttttatggt ataccgagcc tctgaacagg
     1861 gtcgcgtttc tggcaacagc tcgtcccctt cacataaccc ggcagcaaca tccaggaaga
     1921 cctgtctgat gctccttctg gctgctgcct cataaaactc cagcgcggca ccttcaacat
     1981 ggtccagcga gatgtccagg tcaaaaattt caccgtcaaa gcgttctttg tcctgtaagg
     2041 ctacagttac cgtaacttta ttctcaaaat tacggactcc tttcacaacc agttcatagt
     2101 cttgagtcat tggattactc tcctctcgca gccttacgcc tgtcttcttt aatcttgaaa
     2161 taaagatttg tcagatacgt cagcaggcca aaaaccaggc tacccagcac accgattgca
     2221 gcccactgtg acggagttac tttatcgagt aactgcaatg cccagaaacc agcattaccc
     2281 gccgatgtgc cataggcaac acctgttgtt aacttatcca ttgatttcat atcctcaccc
     2341 cgatgtacac ggatggtgca atatgtttga aaagatcgga gtctacgggg tagttttgac
     2401 agcacacgtt gttctcaacg gcgctaaaaa aacatacaca ttaaaaatgt gggtaatta
//
LOCUS       DAJOSY010000240         2411 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJOSY010000240 DAJOSY010000000
VERSION     DAJOSY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.242"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            270..413
                     /locus_tag="OHN27_004431"
     CDS             270..413
                     /locus_tag="OHN27_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCQ2288741.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            426..635
                     /gene="cspE"
                     /locus_tag="OHN27_004432"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             426..635
                     /gene="cspE"
                     /locus_tag="OHN27_004432"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCQ2288742.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            801..1610
                     /gene="rlmA"
                     /locus_tag="OHN27_004433"
     CDS             801..1610
                     /gene="rlmA"
                     /locus_tag="OHN27_004433"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCQ2288743.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEDFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1607..2173)
                     /gene="mntP"
                     /locus_tag="OHN27_004434"
     CDS             complement(1607..2173)
                     /gene="mntP"
                     /locus_tag="OHN27_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCQ2288744.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALVIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          631 a          584 c          592 g          604 t
ORIGIN      
        1 acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt gaactatgtt
       61 agaaactacg ccagtttaag tatctgcctg aactggcaag gttaagcaca atgatatatc
      121 ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga gatttgtgcg
      181 tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt gataacagat
      241 ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa gaagtcctga
      301 gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt gcctcatgca
      361 gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc taaggaattt
      421 ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa ggttttggct
      481 tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct atccagggta
      541 atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag gacggccaga
      601 aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc tgaagtgtga
      661 atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact atgctttatc
      721 ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact tgcggccttt
      781 agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag cctctttcgc
      841 gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg aaagaagggt
      901 atgtcaatct gctgcccgtt cagcataaac ggtctcgtga tccgggcgac agcgcggaaa
      961 tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg cgtgatgcaa
     1021 ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg gatattggct
     1081 gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc accacgtttg
     1141 gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg caggtcactt
     1201 tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac gccataatac
     1261 gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc ggcggctggg
     1321 tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg atttacaatg
     1381 aagtacatct tcatgcacct catgcagaac aactggaaga ttttacatta cagcagagtg
     1441 cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta ttgcagatga
     1501 cgccgtttgc ctggcgtgcg aagccagaag tctggcaaac actggcagca aaagaagtgt
     1561 tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa ccgtggaagt
     1621 gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga atttccgctt
     1681 ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt gacataatca
     1741 aggttgcaca accaatgacc aatgcggtcg cgataatgtt gacctgcagg aaagcaagac
     1801 caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc agtagccaga
     1861 aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa ccctcaataa
     1921 tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc cattcaagga
     1981 caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg gtttcgacgg
     2041 caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg agggtggcac
     2101 ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga agaacagtag
     2161 cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca tcgcccccag
     2221 caaacatgca tacatgaaca acaagccgcg atggttttcg cgttcatgca ggcatagcga
     2281 ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc cacgttttgc
     2341 aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc ccaaatgagg
     2401 gcgcaacctt a
//
LOCUS       DAJOSY010000241         2397 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJOSY010000241 DAJOSY010000000
VERSION     DAJOSY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.243"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..543
                     /gene="traX"
                     /locus_tag="OHN27_004435"
     CDS             <1..543
                     /gene="traX"
                     /locus_tag="OHN27_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HCQ2288745.1"
                     /translation="PLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQFAYYLAGFPWY
                     EGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGSSYGIAGLLMLAVSHRL
                     YRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSCAGKSLPRFWP
                     GDFFPVFYACHLAVLGVLAP"
     gene            598..1158
                     /locus_tag="OHN27_004436"
     CDS             598..1158
                     /locus_tag="OHN27_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCQ2288746.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEK"
     gene            1289..1501
                     /locus_tag="OHN27_004437"
     CDS             1289..1501
                     /locus_tag="OHN27_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288747.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAAHCHRRCRGRV"
     gene            1743..2204
                     /locus_tag="OHN27_004438"
     CDS             1743..2204
                     /locus_tag="OHN27_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCQ2288748.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            2250..2397
                     /locus_tag="OHN27_004439"
                     /pseudo
     CDS             2250..2397
                     /locus_tag="OHN27_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; frameshifted;
                     incomplete; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT          601 a          540 c          713 g          543 t
ORIGIN      
        1 ccgctgtttg ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg
       61 gccattaacc ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggccggc
      121 tttccctggt atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg
      181 tggtgtgaaa cgcgcagtgg gtggcgtaca gcagcggcca ttcttctgat ggccctgtgg
      241 gggcctttgt ccggctccag ttacggcatt gccgggctgc tgatgctggc agtcagccac
      301 cggctgtacc gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg cctgctggct
      361 gttattccgg cgcttaacct tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg
      421 acggtgctga ccgttggtct ggtgtcgtgt gcaggaaaat cattaccccg tttctggccc
      481 ggggattttt tcccggtatt ctacgcctgt catctggctg tgctgggcgt tctggcgccg
      541 tgacgggtgt ggtatctttg accgtaacag gatgattcgt cagaggcaga acacagcatg
      601 acagagcaga agcgaccggt actgacactg aagcggaaaa cggaagggga gacgcctgtc
      661 cgcagccgga aaaccatcat caatgtcacc acgccaccaa agtggaaggt gaaaaagcag
      721 aagctggccg agaaagcagc ccgggaagca gagctggcgg caaaaaaagc gcaggccaga
      781 caggcactgt ccatttatct gaacctgccc acgctggatg aggccgtgaa taccctgaaa
      841 ccctggtggc cgggattgtt tgacggtgac acgccccggc ttctggcctg cggtatccgg
      901 gacgtgttac tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc
      961 agggcgctga aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc
     1021 tgtcggtatg acacggaagg gtatgtaacg gagcatattt ctcaggagga ggaagtgtat
     1081 gcggcagagc gtctggataa aatccgccgc cagaaccgga taaaggcaga acttcaggcc
     1141 gtgcttgatg agaaataaaa aagcctccgt cagagagagg aggcagggaa ataaggctta
     1201 aaggaaataa cttccatgcc cgaaaggaaa tcggtccact gaattattta acaccataat
     1261 gctgacgggt taaagaggag tgataaagat gaatggattc agaaacagtt cacggaacgg
     1321 tcaggtctgg cgctggcagc gtgccggaag ccgggccgtt attctggagg tcagtggacg
     1381 ctggatggaa gcggcagaag catggcgacg ggctgccggt gttgcgcccc ggacagactg
     1441 gcagcagttt gcccgaaaaa gggctgcaca ctgccaccgg cgctgccggg gaagggtgta
     1501 acgaaaaaag cagccttcat tactccagga gtcagaaggc tgaataatgc atgagattta
     1561 cttttttaat ctccggtcgg gcagtatgac cggacagttc tgagtagcac agagtttttt
     1621 ccgtattaag tcgtgatttt atattttgtg attaatttca caaaataagg tcttattcaa
     1681 tgtgtgctgc gatattcagg ggggtgaaat acaggtgcca tttatctgat atggagaata
     1741 acatgaggaa atatattcca ctggtattat ttatcttttc atggccggta ttgtctgcag
     1801 atattcatgg acgggttgtt cgtgtactgg acggtgatac catcgaggta atggactcac
     1861 ggaaggcggt gcggatcagg ctggtaaata ttgatgcacc ggaaaagaaa caggattatg
     1921 gccggtggtc aacagacatg atgaaatcac tggtcgcagg aaaaacggtt accgtcacat
     1981 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
     2041 atattaatca gtttatggtt cgggccgggg ctgcctgggt atatgagcag tacaacacag
     2101 atcccgtgct gcctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
     2161 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatctta
     2221 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
     2281 agttacggcg atgttcatca gtcaatacgc tggaaaaatt tttcataaaa acagagacag
     2341 tttatcaaac agtgaacgtg aatcatttaa ttcagcagca gaccaccggt tggcgga
//
LOCUS       DAJOSY010000242         2329 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJOSY010000242 DAJOSY010000000
VERSION     DAJOSY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.244"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            94..408
                     /gene="fliE"
                     /locus_tag="OHN27_004440"
     CDS             94..408
                     /gene="fliE"
                     /locus_tag="OHN27_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCQ2288749.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            742..1074
                     /gene="emrE"
                     /locus_tag="OHN27_004441"
     CDS             742..1074
                     /gene="emrE"
                     /locus_tag="OHN27_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCQ2288750.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPKGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            1243..1794
                     /gene="ybcL"
                     /locus_tag="OHN27_004442"
     CDS             1243..1794
                     /gene="ybcL"
                     /locus_tag="OHN27_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCQ2288751.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            1804..>2329
                     /locus_tag="OHN27_004443"
     CDS             1804..>2329
                     /locus_tag="OHN27_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCQ2288752.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEP"
BASE COUNT          649 a          467 c          522 g          691 t
ORIGIN      
        1 gccaataaac ccgttttttt gttgctattt agcgcctttg tcttattgac ttactggtag
       61 gctttgctac cagaaattat ccaggagacg aaaatgtcag cgatacaggg gattgaaggg
      121 gttatcagcc agttacaggc tacggcgatg agtgcgcgtg cgcaggattc actgccgcaa
      181 ccgaccatta gttttgccgg gcagctgcac gccgcgctcg atcgcattag cgatacacaa
      241 acagctgccc gcacgcaggc agaaaaattc accctcggtg aacccggcgt ggcgttaaac
      301 gatgtgatga ccgatatgca aaaagcctca gtttctatgc aaatggggat tcaggtgcgt
      361 aataagctgg tggcggcgta tcaggaagtg atgtccatgc aggtgtaggt tttgtaacct
      421 gttgttaatt acttccaatc tcaccgcttg gggcatggat ggggcaaact cactcaattt
      481 ctggtttaaa atggatacct gatcctgaaa gttttctgcc atccacgatc cgtaaacgcg
      541 ataacatgtt ttgtcttcgc tttgctcata ggaagagtga actcttttgt tagcctgggg
      601 ttccttctga tcattattgt tcctgccttt atctcttgcc acgccagagc cccctggata
      661 cacatacacc aggatgaaaa cggtgagttg tgttcaaact gtagtacaat tctctccagt
      721 atgaacagga aagaatatgc tatgaaccct tatatttatc ttggtggcgc aatacttgca
      781 gaggtcattg gtacaacctt aatgaagttt tcagaggggt ttacacggtt atggccctct
      841 gttggcacaa ttatttgcta ttgtgcatca ttttggttat tagctcagac actggcttat
      901 atacctaaag ggattgctta tgctatctgg tcaggagtcg gtatcgttct gattagtttg
      961 ctttcctggg gattttttgg ccaacgactg gacttgccag ccatcatagg catgatgttg
     1021 atttgtgccg gtgtgttagt cattaacttg ttgtcacgaa gcgcaccgca ttaaaaaata
     1081 atcatccttg agataacagg aatctgattt gtgcatatta tggctaatgt tcggggcagc
     1141 tttataatta tgattgcatt tgcgcgatca ttgcgccttt ttgcataatg agatcaatgc
     1201 acatcaaata aaataacgat aggtcacaac aaagaggttt ttatgaaaaa acttatcgtt
     1261 tcaagtgtat tggcattcat aacattttct gcgcaggccg cagcatttca ggtcactagt
     1321 aatgaaataa aaacaggaga gcaacttaca acgtctcatg tattttctgg atttgggtgt
     1381 gaggggggta atacatcgcc ctcattaacc tggtctggtg ctccagaagg caccaaaagc
     1441 tttgccgtaa ctgtgtatga tccagatgcg cctacaggca gcggatggtg gcactggact
     1501 gttgctaata ttccagcaac ggtaacatat ttgcccgctg atgcaggaag acgtgatggt
     1561 acaaaacttc cgactggtgc tgtccaaggc cgaaatgatt ttggctatgc gggatttggt
     1621 ggagcatgtc ctcccaaagg agataaaccc catcattacc agtttaaaat atgggctcta
     1681 aaaactgata agattcctgt ggattctaat tccagcggag cgttagttgg ttatatgctt
     1741 aatgccaata aaattgcaac cgctgagata atacccgttt atgaggtaaa gtaggataag
     1801 agtatgctgg caaaagatag gactaaccta aagattgaag aaatacggat gcataaacat
     1861 catgagattc acagggttaa gcctcttatg ccagctttat gtcgtatccg tcagggaaaa
     1921 aaagttatca attgggagac gcatagtcta actgttgata ataatcagat aatattattt
     1981 ccctgtggat atgaatttta tattgcgaat tacccagaag caggtcttta tcttgcagaa
     2041 atgctttatt atcccattga tctaattgaa aagttccaaa atctttatgc gataaccgat
     2101 caaattcgta acacgacagg tttctgctta cctcagaatc ccgagttaat atattgttgg
     2161 gagcaactaa aaacatctat ttcccgaggc ttctcaacgc aaattcagga acacttagca
     2221 atgggtgttc tactttcatt aggagcacat catgttaatt gtttactttt atcagatagt
     2281 aaacaatcat taataagtcg ttgttataac ctaatgctat ccgaacctg
//
LOCUS       DAJOSY010000243         2315 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJOSY010000243 DAJOSY010000000
VERSION     DAJOSY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.245"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(56..1339)
                     /gene="ydfJ"
                     /locus_tag="OHN27_004444"
                     /pseudo
     CDS             complement(56..1339)
                     /gene="ydfJ"
                     /locus_tag="OHN27_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1943..2056
                     /locus_tag="OHN27_004445"
     CDS             1943..2056
                     /locus_tag="OHN27_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288753.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            2125..>2315
                     /gene="ydfK"
                     /locus_tag="OHN27_004446"
     CDS             2125..>2315
                     /gene="ydfK"
                     /locus_tag="OHN27_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HCQ2288754.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTA"
BASE COUNT          717 a          549 c          434 g          615 t
ORIGIN      
        1 atgttttgtt atatgaataa aaatcccctc tccggtaaga gaagggattg aggatttaca
       61 gacttctgga aggttgcgca gctcttacaa cacgcggttg atcttccgcg gcgtcttcca
      121 gcgcacttaa atcacggtct ttcacttctg gcattttcag cgcagagatt aaaccaatca
      181 ctgaatatgc catgatcata atggcgatcg gataccagga ttccgtcatg gtgcagaaaa
      241 tacccgccag gataggacca aaaccggaag cgataagacc accaatttct ttagaaatag
      301 ccatccgggt aaagcggttt ttacagccga acatttctgc catggtaata ttttccagag
      361 caaataatcc cagcaccgca cagttatgaa tcacaatcag tgcaaccata atggtgctcg
      421 gggcatagct tttatctacg atgatagaaa gcattggcca tgccagcaca atcgcggagg
      481 tattcataat aatatacggg atccggcgac caattttatc ggataaccaa ccaaggaacg
      541 gaatggtcat aaagccgaga atcgaactga tcatcaatgc atctgttgga attgctttgt
      601 taaacaataa cgtctgcact aaatagcccg caaggaaagt ctgaattaaa cctgagttac
      661 ccgcctgacc aaaacgcagc cctgttgcca gccagaagga tttgctctgg aacatgctac
      721 cagcaggtgc aggttttgct gtcggttggt tgctgtcgtt aaccttctca aaaaccgggc
      781 tttctttcag attcatacgc aaccagatag caaagaccat cacgacaacg ctcgccagga
      841 acggtatacg ccatccccac gccagcagtt cctctttact gagaatgaag aacataaagg
      901 cccagattgc cgttgcgctt aaagttccgc agttagttcc catagccaca aatgaggaga
      961 taattccgcg cttacctttt ggcgcatatt ccgccagcat cgtaccggca ccggaaattt
     1021 ccgcacctgc acccaatccc tgaataatac gcagcgtcac cagcaagatg ggggcaaaaa
     1081 caccaatctg tgcataggtc ggtaacacac caattaaggt ggtacagatc cccatcatgg
     1141 tgatggtaat aaagagcact tttttacgcc ctattctgtc gcccattttg ccgaaaataa
     1201 atgctccgac aatacgcgcc acataacctg caccgtaggt tcccattgcc agaattaacg
     1261 ccattgccgt tgatgattca ggaaaaaata tttcatgaaa cactaacgct gcgccgagcg
     1321 aatataactg gaaatccata attcacaggt gttttttccc atcctgtggt ttccttggcg
     1381 ttttctaggt tttttcagat agttgcattt tttaaaaagc atcctaagtt cgatctctgt
     1441 gtctatctgg ggcctatttc tgtcccatat atgccccaaa aaaactcccc aacagataag
     1501 tagttttttc atggatttat gcgtaaaatc aagaacggct ggaaatcatt caatactcac
     1561 actatcgaaa aatttaccag ccaaccgcag caccttcttg cataaggcgt gtctgcggtt
     1621 tttcaactat tcagatacat cactcccatc acattcattc ctccgcatca aaggcatata
     1681 ggctatatca ccttgatatt tttctttttc agataaaaac tgttatctat gtatactttt
     1741 aaacccaatc cgtgtagagt ctctgcataa gatagtttgc agttgccgct tcagcttgcg
     1801 ccataaaccg cctgattttt gctgccacct gttagcattc ctgtatacct gaaacgacaa
     1861 tgtttatcta cgaactttaa gaacacccaa gataaaaatt gtcaactata tcatatataa
     1921 cacattacta attcgaggct atatgaacag catactgata atcacatctc tccttatcat
     1981 attcagcatt tttagtcatg ccctaataaa attagggatt ggcatatcca ataacccaga
     2041 caaaaccgat gtataagtca acatatcctg aatcagacat acaatatcgc aatgaaaatc
     2101 aataatattt taaggaatat cttcatgaaa tcaaaagaca ccctaaagtg gttccctgcg
     2161 cagcttcctg aagtaagaat tatcctaggg gatgctgtag tggaagtagc aaaacaggga
     2221 agacctatca ataccagaac attgcttgat tacattgaag gaaacataaa gaaaaaatca
     2281 tggctggata acaaagaatt attacaaaca gcgat
//
LOCUS       DAJOSY010000244         2310 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJOSY010000244 DAJOSY010000000
VERSION     DAJOSY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.246"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            361..558
                     /locus_tag="OHN27_004447"
     CDS             361..558
                     /locus_tag="OHN27_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HCQ2288755.1"
                     /translation="MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPH
                     DMVESSAAAWRDEMSRKVAGK"
     gene            555..737
                     /locus_tag="OHN27_004448"
     CDS             555..737
                     /locus_tag="OHN27_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2288756.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLF
                     SLSFISVQVHDNIITV"
     gene            836..1144
                     /locus_tag="OHN27_004449"
     CDS             836..1144
                     /locus_tag="OHN27_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288757.1"
                     /translation="MDSKKMWRSNYAPPLLRILWRLGIRLPPLPFMPFWQITLLMGGL
                     WGISWGCAMWFMYWGPSGMVAGEAIIISITSGFLFGLLMASFHWWRRKVNRLPPWNDV
                     "
     gene            1704..>2310
                     /locus_tag="OHN27_004450"
     CDS             1704..>2310
                     /locus_tag="OHN27_004450"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCQ2288758.1"
                     /translation="MQSASKLIYRGKLLGSLPTVGEIHKEIAVSEETVTWISLQRDII
                     ANILLGKDPRLLVIVGPCSIHDVQAAVDYAKRLFVLQNKYLSKIYIVMRTYFEKPRTR
                     KGWKGIMHDPDLNGSYDVEKGIRYARQCLSSITTMRVATATEFLDPFLTPYIADLICW
                     GAIGARTTESQTHRQLASGLHCPVGFKNSTDGNINLAIDAII"
BASE COUNT          586 a          514 c          599 g          611 t
ORIGIN      
        1 atgttaaagt gtcatatcgt ttacataaag tcaagatata tcagtgtggc aagcctggca
       61 ggattctcta gggaagggca ggcatactca gtgcatagct gatgcacgga tgacggcagg
      121 gggaggaggc tggtgacttt cggacgactg tagcggctta catcgggctt cggcggtatc
      181 atatgaaaca gcggagtgcc gccttccatg ccgctggcgc ggcatatcct agtgacgata
      241 tctgaatcgt tatatactgt gtatatacgt ggtaatgacg gggtgataaa tggcacaggt
      301 taatatgagt ttaagaattg acgctgaact gaaggatgct tttatggccc tgcaaaaagc
      361 atggaccgta atggctctca gttaatccgg gattttatgc gccagaccgt ggaacggcag
      421 cataatacct ggttccgtga ccaggttgag gcaggacgtc agcaactcga gcgcggcgat
      481 gtgcttcccc atgacatggt cgaatcttcc gctgctgcat ggcgggatga aatgagcagg
      541 aaagtcgccg gtaaatgatg gagatattct ggaccatgct ggccagccag gacaggaagc
      601 gcattcgtga gtacattgcg gagcagaacc tgatagccgc cattgaactg gacgaacgga
      661 ttggttgttc agccagcctc ctgttctccc tttcttttat cagtgtacag gtgcatgaca
      721 acataatcac ggtatgatga taataatcaa taacaataag ttgtgtcacg tttacatcat
      781 ggaacggagg tgctcctgtg acttttgagc aaaaaaagcc cgggcaattg ccctgatgga
      841 cagcaaaaag atgtggcgga gtaattatgc tcctcctctg ctgcgtattc tctggcggct
      901 gggtatccgg cttccgccat tgccctttat gccgttctgg cagattacgc tgctgatggg
      961 gggcttatgg ggtatttcct ggggatgtgc aatgtggttt atgtactggg ggccgtcagg
     1021 aatggtagca ggtgaggcca ttattataag tatcacgagt ggtttcttgt tcggactgct
     1081 catggcctct ttccactggt ggcgcaggaa agttaaccgg ctgccaccat ggaatgatgt
     1141 gtaaaccgtg ccggcatgtt tttacggggt gatgctttca ttaacctgta cacgaacttc
     1201 tattcctgca tcattacagg cctacagcca ctacgccact tccagcggat cgccctccct
     1261 gcgtacctcc ccgccttctt tattctataa ctgcagacag gtgctgccgt cgaggcgcac
     1321 cacaaaatcc ccacggcagg cctgatatgt catgccgttc agctccaccg gcagatcagt
     1381 ggcagaagtg ccatctgcgg gcagacctga tgccgggacc agcggcagta cgacgacttc
     1441 tgcagtacgc tgttctgcca gcagagaggc tgtgccacgg cccggtcagc ttttgtgagt
     1501 tccacagcta gccgcagctc agggattaag attcaggtaa tttacggaaa acaagtgatt
     1561 tccgtacggg agagaaggcc taacgtatat tatctcagaa atgactatga atccccatat
     1621 attgccaatt atacagcaaa tgattatcat tatcatctac tgtgtgcaac tgatggtctg
     1681 aaatagcgct tttaggagac aagatgcagt cagctagtaa attaatctac cgcgggaagt
     1741 tgcttggcag tttacccact gtgggcgaga ttcataaaga aatcgcagta tcggaagaaa
     1801 ccgtaacatg gatctctctg caacgagata ttatagcgaa tattttgctg ggaaaagatc
     1861 ctcgcttact tgtgattgtt ggtccatgct caatccatga cgttcaggcc gcagttgatt
     1921 atgcgaagcg attgttcgta ttacagaata agtacttatc gaaaatatac attgtaatgc
     1981 gcacttattt tgagaaacct cgaacccgta aaggctggaa gggaataatg cacgatcctg
     2041 atctaaatgg aagttatgat gttgagaaag ggatacgata tgctcgccag tgtttgtctt
     2101 caatcacaac gatgcgtgtc gcaaccgcta cagaattcct cgatcctttt cttactccct
     2161 atattgctga tttaatatgt tggggtgcta ttggtgccag gaccactgaa tcacaaacgc
     2221 atcgtcaact tgcgtctggt ttacattgtc cagttggatt taaaaacagt actgatggaa
     2281 atattaactt agctattgac gctattattg
//
LOCUS       DAJOSY010000245         2252 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJOSY010000245 DAJOSY010000000
VERSION     DAJOSY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.247"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            170..1030
                     /locus_tag="OHN27_004451"
     CDS             170..1030
                     /locus_tag="OHN27_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HCQ2288759.1"
                     /translation="MDEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVE
                     ALNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDD
                     CCSLLASFAASLPLRRPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEA
                     INHRTLSMAC"
     gene            1132..>2252
                     /gene="istA"
                     /locus_tag="OHN27_004452"
     CDS             1132..>2252
                     /gene="istA"
                     /locus_tag="OHN27_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family transposase"
                     /protein_id="HCQ2288760.1"
                     /translation="MINVAILSAIRRWHFRDGASIREIARRSGLSRNTVRKYLQSKVV
                     EPQYPARDSVGKLSPFEPKLRQWLSTEHKKTKKLRRNLRSMYRDLVALGFTGSYDRVC
                     AFARQWKDSEQFKAQTSGKGCFIPLRFACGEAFQFDWSEDFARIAGKQVKLQIAQFKL
                     AHSRAFVLRAYYQQKHEMLFDAHWHAFQIFGGIPKRGIYDNMKTAVDSVGRGKERRVN
                     QRFTAMVSHYLFDAQFCNPASGWEKGQIEKNVQDSRQRLWQGAPDFQSLADLNVWLEH
                     RCKALWSELRHPELDQTVQEAFADEQGELMALPNAFDAFVEQTKRVTSTCLVHHEGNR
                     YSVPASYANRAISLRIYADKLVMAAEGQHIAEHPRLFGSG"
BASE COUNT          480 a          671 c          658 g          443 t
ORIGIN      
        1 gcgcgcagaa ggccacacgc aaggcgcggc gtgacgcgga tcgccgccag cacctcaaga
       61 catcagctcg gccggacaag cccgttccgc cggatacgga tattgccgac ccgcaggcag
      121 acaacttgcc acccgccaaa ccgttcgacc agattgagga gtggtagccg tggacgaata
      181 tcccatcatc gacctgtccc acctgctgcc ggcggcccag ggcttggccc gtcttccggc
      241 ggacgagcgc atccagcgcc ttcgcgccga ccgctggatc ggctatccgc gcgcagtcga
      301 ggcgctgaac cggctggaag ccctttatgc gtggccaaac aagcaacgca tgcccaacct
      361 gctgctggtt ggcccgacca acaatggcaa gtcgatgatc gtcgagaagt tccgccgcac
      421 ccacccggcc agctccgacg ccgaccagga gcacatcccg gtgttggtcg tgcagatgcc
      481 gtccgagccg tccgtgatcc gcttctacgt cgcgctgctc gccgcgatgg gcgcgccgct
      541 gcgcccacgc ccacggttgc cggaaatgga gcaactggct ctggcactgc tgcgcaaggt
      601 cggcgtgcgc atgctggtga tcgacgagct gcacaacgtg ctggccggca acagcgtcaa
      661 ccgccgggaa ttcctcaacc tgctgcgctt cctcggcaac gaactgcgca tcccgttggt
      721 tggggtaggc acgcgcgacg cctacctagc catccgctcc gatgaccagt tggaaaatcg
      781 cttcgagccg atgatgctgc cggtatggga ggccaacgac gattgctgct cactgctggc
      841 cagcttcgcc gcttcgctcc cgctgcgccg gccttcccca attgccacgc tggacatggc
      901 tcgctacctg ctcacacgca gcgagggcac cataggggaa ctggcgcact tgctgatggc
      961 ggcggccatc gtcgccgtgg agagcggcga ggaagcgatc aaccatcgca cactcagcat
     1021 ggcctgttga gttgcatcta aaattgaccc acttagggta aagatttgcg tcgaaatttg
     1081 acccacgtat gacactgttt cccgtctgga tatggcggga gaaatcaagg agtgataaac
     1141 gtggcgatat tgagcgcaat tcgacgctgg cattttcgcg atggtgcgtc gattcgggaa
     1201 atagcccgac gaagcggcct gtccaggaac accgttcgca agtatttgca aagcaaggtg
     1261 gttgaaccgc agtacccagc gcgagacagc gttggcaagt taagtccttt tgagcccaag
     1321 ttaaggcagt ggctctccac cgagcacaaa aagacaaaga agctgcgcag aaacctgcgc
     1381 agcatgtacc gggatttggt cgctttgggc tttaccgggt cttatgaccg agtgtgtgcc
     1441 tttgcccgac agtggaaaga ttccgaacag ttcaaggcgc aaacctcggg caagggttgt
     1501 ttcatcccct tgcgctttgc ttgtggcgaa gccttccaat tcgattggag tgaggacttt
     1561 gcccgcatag cgggcaaaca ggtcaaactt cagattgccc agtttaagtt ggcccacagc
     1621 cgggcctttg tgcttcgggc ttactaccag caaaaacatg aaatgctgtt tgatgcccac
     1681 tggcatgcct ttcaaatctt cggtggcatt cccaagcgcg gcatctacga caacatgaag
     1741 accgctgtgg attcggtggg gcgtggcaaa gagcgcaggg tcaatcagcg gttcactgcc
     1801 atggtcagcc actacctgtt tgatgcgcag ttctgtaatc cagcatcggg ttgggagaaa
     1861 ggccagattg agaagaacgt gcaggattcc cgccaacgcc tgtggcaagg ggcaccagac
     1921 tttcaaagcc ttgctgattt gaatgtgtgg cttgagcatc gctgcaaagc gctgtggtct
     1981 gagctgcgcc accccgaatt ggaccaaacc gtgcaagagg cctttgccga tgaacaaggc
     2041 gagttgatgg cgctacccaa tgcctttgat gcattcgtgg agcaaaccaa gcgagtcact
     2101 tcaacctgcc ttgttcacca cgagggcaat cgctacagcg ttcctgccag ttacgccaac
     2161 agggccatca gccttcggat ttatgcagac aagctggtga tggctgccga aggccaacac
     2221 attgccgagc atccaagatt gtttggcagt gg
//
LOCUS       DAJOSY010000246         2196 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJOSY010000246 DAJOSY010000000
VERSION     DAJOSY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.248"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..447
                     /gene="traN"
                     /locus_tag="OHN27_004453"
     CDS             <1..447
                     /gene="traN"
                     /locus_tag="OHN27_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HCQ2288761.1"
                     /translation="GNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLRIPRQG
                     PTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGDDLLVR
                     SRAILRDEFEKTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            450..1739
                     /gene="traO"
                     /locus_tag="OHN27_004454"
     CDS             450..1739
                     /gene="traO"
                     /locus_tag="OHN27_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HCQ2288762.1"
                     /translation="MSAEQDAGKSGKKLAALLGLSSIILFGGSYIAFSKVSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAYRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            1739..>2196
                     /gene="traP"
                     /locus_tag="OHN27_004455"
     CDS             1739..>2196
                     /gene="traP"
                     /locus_tag="OHN27_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HCQ2288763.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSR"
BASE COUNT          616 a          549 c          576 g          455 t
ORIGIN      
        1 ggaaacattt ctgtctacct gaaaggtctg tcggtaccgg tcatcctgaa cgtaaccagt
       61 ggcgaaacag atacgccgtc atccagtcag gaaatggaca gtcgtctgga tctgcgaata
      121 ccacggcagg gaccaaccag cccggttgtc tccatcccga cggataaaat cgccctgcat
      181 gatgcaacat tacaggcatt tctggatggt attcctcccc gggacccttc agttaagagg
      241 ctgaaattca caggtaatgt cccggataca acgatctggc aacacggcga tgaccttctg
      301 gttcgttccc gggcgatatt gcgcgatgaa tttgaaaaga ccttgtcctc tgccgacgga
      361 actcatctct ggaagcttcc cgtcacccca ctactgactt tctctgtgaa tggccagtcc
      421 gttcacgtga caccagaact ggagtaatca tgagtgctga acaggatgcc ggtaaatccg
      481 gaaagaaact ggctgccctg cttgggctga gcagcatcat cttatttggc ggcagctata
      541 tcgcgttttc caaagtatca gggagtaaca gcgatatgca gtcagccgtg aacataaaca
      601 gtgctgcaag tggcggtacg cgcagcgtta ccgaaacgcc tcactatcgt gaactgttaa
      661 gagcggataa tgaacgaggg gctgcggcgg cagcccgcaa taatcagacg ttcattgcca
      721 gtcttccgca ggggcttgat attccggaca ctccggaaaa gcaacaacag cctgcagcaa
      781 aaccggaaaa ctatgcttat cgccaggctt ccggcacacc acaggaggat cgagcagcca
      841 gtgaaaagcg tatggaacgg ctgcaaaagc tgattgtaag aataaaagat cagcatcctg
      901 ccggtagtac accaaccata gcaacgacta tgtggaataa atctccggca gaaacaacag
      961 ggcagaatgg tacgcagcaa ttcgcattac agaatgccag cctgtccacg cctgtagcag
     1021 aaaaagggat acaactgata cctgccctga cccgtatacc ggcatatatt gatactgcag
     1081 tggattctga taacccgtca tccaaggtta tcgcaaccat tcccgctggc ccctgggcag
     1141 gggcaacgct gttcagtccg ggagtcaaac tggtgggtaa cggcgtggag atccatttcg
     1201 accggatgag ctggaacggc atggacctta aggtcaatgc atatgctcaa cgggaagaca
     1261 acctgatgtc atccgtggcc agcaatgtga atacacgctg gtttaaacat atcattctgc
     1321 cgtcagtgtt aggaggtgtg ggcagtattg gcacacttta taaagatgcc aacacgcagg
     1381 ttattcaggg taactatggc acggttaccg gacgcgtggg aatgcccagt ggtgaagctg
     1441 ttgcgggcgt tattgccggt ggtatggcgg aaagaggaag tcagatatta acccgccagg
     1501 cagaaagtga gccctataaa caggtagaag tgtatcagca tgaagtcgta tcaattctgt
     1561 ttgttgaccc ggtaatgaca aacgacgcca ggtcatcttc cctttccagt ggcatttccc
     1621 cttcagttaa tcgtacatca caggcggaac aacgctcgca ggcacgaatg cagacagcca
     1681 tggagcaaag aaaagctgtg atgcaacgtc ggtatgatga acaaccggag acaccataat
     1741 gaaacctgaa acagaaatag atgaaaccgg cagcttcggt gaacccgaag agaaacccgc
     1801 ccccttctgg aaacgttccg tctgggggat atctgtagca acatgggggc tatgtgcagt
     1861 cgtgttgctc gcagccatat ggtatctgtt cctgagagcg ccatcggaaa caggcatgcc
     1921 tccgtttaat gatgcggatg ctggcgtaca gacctggcaa accacacaag aatcatcacc
     1981 ttccgttcag tccactgaga ctatgactgt ccggacagga gaagatatga gccagcttgc
     2041 ccgggatgta aaaacagagc tggataaccg cgatgaaaaa attcaggcaa cactgaatat
     2101 gcttcacgac agcattaaca agctgggcga agctataaaa aaggatgaag aatacgcaca
     2161 ggaaacacgc cgtcagctgg atgatatccg gagccg
//
LOCUS       DAJOSY010000247         2158 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJOSY010000247 DAJOSY010000000
VERSION     DAJOSY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.249"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(36..1442)
                     /gene="gndA"
                     /locus_tag="OHN27_004456"
     CDS             complement(36..1442)
                     /gene="gndA"
                     /locus_tag="OHN27_004456"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCQ2288764.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPGKKLVPYYTVQEFVESLETPRRILLMVKAGAGTDSAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILKQIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYALLKGGLALSNEELAQT
                     FTEWNEGELSSYLIDITKDIFTKKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAQNAGIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAISYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLG"
     gene            complement(1560..>2158)
                     /locus_tag="OHN27_004457"
     CDS             complement(1560..>2158)
                     /locus_tag="OHN27_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HCQ2288765.1"
                     /translation="GFLATSYDVGIYTSAQKIVMLFISAIITPLSFIVFPALSKRFGV
                     SVEHGLNSFRKFMPLLCMCCFGSFLFIFFFGGKVILIMMGAQFKDSINVLNVLSFGYV
                     FVFWGAVIGGQIVLNLKYDKQFVQMQWFVAFLSFIFNVLFLKKYGAMGLAYIWTISEF
                     IMTFMQCVFIKYKGIRIIDWEQFKIKNMITNFKNLLRH"
BASE COUNT          666 a          492 c          442 g          558 t
ORIGIN      
        1 aaaatcaggg ccctaaattt agttaaagat ataaattaac ccaaccactc agtatggaat
       61 acaccttctt tatcggtacg tttataggta tgcgcaccaa agtaatcacg ctgagcctgg
      121 atgaggttag ctggcagaac tgcggaacga taactgtcgt agtaggaaat agcagcagag
      181 aatgtcggaa ccgggatgcc attctgtacg gcataggcta caacatcacg cagtgcctgt
      241 tgatagtcat cagcgatctg tttgaagtat ggagccagta gcagattagc aatgccggcg
      301 ttttgcgcat aagcatcggt gatcttctgc aggaactgcg cacgaatgat gcagccagcg
      361 cggaaaatct tagcgatctc gccgtagtta agatcccagt tgtattcatc agaagcagca
      421 cgcagctggg agaagccctg agcataggaa acgattttcc cgaggtacaa cgcacgacgt
      481 actttttcga taaattcagc tttatcacca gccggctgag cctgcggacc actcagcact
      541 ttagaagcgg ctacacgctg gtctttaagc gatgagatgt aacgagcaaa tacggactcg
      601 gtgatcagag acagaggttc gccgagatcc agtgagctct ggctggtcca cttaccggta
      661 cctttgtttg ctgcttcatc aagaataaca tcgacaaggt atttaccctc ttcatccttc
      721 ttggtgaaga tatctttggt aatgtcgata aggtagctgc tcagttcacc ttcattccat
      781 tcagtgaaag tttgcgcaag ctcttcgttg gagagcgcca ggccgccttt caacagcgca
      841 taagcttcgg caatcagttg catgtcacca tactcgatgc cgttatgaac catcttcaca
      901 taatggccag caccgtcagc accgatatag gtcacacatg gctcaccgtc ttctgcaacc
      961 gcagcaatct gcttcagaat tggagcaacc agctcataag cttctttctg gccaccaggc
     1021 ataatggaag gccctttcag agccccctct tcaccaccgg aaacaccggt accgataaaa
     1081 ttaaaacctt cggcagagag ttcacgattt cggcggatgg tgtcctggaa gaaggtgttg
     1141 ccaccatcaa taatgatgtc gcctttatca aggtagggct tcagggaatc gatagcgcta
     1201 tcggtgccag ccccggcctt caccatcaac aggatacgac gtggtgtttc gagggattca
     1261 acaaactctt gtactgtgta gtaagggact agtttcttgc ctgggttttc ggcaatcact
     1321 tcttcggtct tttcacggga gcggttgaaa acggagacgg tataaccacg gctttcgatg
     1381 ttcagcgcaa ggttacgccc catcacagcc ataccgacaa ctccgatctg ttgcttggac
     1441 attacatact cctgtcaggt gactattttt atcgaaacaa ctattagttt aactcaatga
     1501 tgataagacg tatataaaaa aagaaactct agagagtaaa acatacacac aagtaaaaat
     1561 tagtgtcgta acaaattttt aaagttagtt atcatatttt ttattttaaa ttgttcccag
     1621 tcaattatcc gtatgccttt gtatttaata aaaacacatt gcataaaagt cataataaac
     1681 tcagatatgg tccaaatgta tgccaagccc attgcaccat atttttttaa gaaaagaaca
     1741 ttgaatataa aacttaaaaa agctacaaac cattgcattt gaacaaattg tttgtcatat
     1801 tttaagttta aaactatttg cccgccaatt actgcccccc agaaaacaaa aacatatcca
     1861 aaagacaaca cattcagaac gttaatgcta tccttaaatt gtgcacccat catgatgagt
     1921 atgacttttc caccaaagaa aaaaataaat aaaaatgaac caaagcagca catacataat
     1981 agaggcatga actttctgaa agaattcaaa ccatgttcaa cactgacacc aaatctttta
     2041 gataatgcgg ggaaaacaat gaaagataaa ggcgtaatga tcgcgctaat gaatagcata
     2101 actatttttt gtgccgaagt atatatcccc acatcataag acgtggcaag aaacccca
//
LOCUS       DAJOSY010000248         2075 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJOSY010000248 DAJOSY010000000
VERSION     DAJOSY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.250"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1118
                     /gene="nikB"
                     /locus_tag="OHN27_004458"
     CDS             <1..1118
                     /gene="nikB"
                     /locus_tag="OHN27_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HCQ2288766.1"
                     /translation="HGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFVSAPADL
                     FDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
                     PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDE
                     RQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVL
                     CEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADK
                     LDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITRPDVDHH
                     REGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(1155..1880)
                     /locus_tag="OHN27_004459"
     CDS             complement(1155..1880)
                     /locus_tag="OHN27_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="icmO-like type IV secretion system domain
                     protein"
                     /protein_id="HCQ2288767.1"
                     /translation="MNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
BASE COUNT          462 a          513 c          569 g          531 t
ORIGIN      
        1 atcacgggct tgttgtcgtt gatgctttta atcatgagca aacgccggtg aaagccagca
       61 gcattcatcc tgatctgaca ctggggcgtg cggagccaca ggccggacct tttgtgagtg
      121 ctccagcaga tttgtttgac agggtgcaac ctgaaaatcg ctataacccg gagctggctg
      181 tcagtgacag gtacggggtc agcagtaaac gtgacccaat gctgcgccgg cagcgacgtg
      241 aagccagggc tgaggcccgt gctgacctgc gtgcccgcta tctggcatgg cgtgaacaat
      301 ggcgtaaacc ggatctgcgt tatggggaac gttgtcggga aattcatcag gcatgccgtc
      361 tgcggaagtc acacattcgt gcgcagtacg atgatccggc attacgtaag ctgcattatc
      421 acattgcaga agttcagcga atgcaggcat tgatcaggct gaaagaagac atcagggatg
      481 agcgacagaa acttattgct gacgggaaat ggtatccacc ctcttaccgt cagtgggtcg
      541 aaattcaggc tgctcaggga gacagggctg ctgtatcgca gttgagaggc tgggattatc
      601 gcgatcgccg taaagacaag tcacgcacaa cgacgacaga ccgctgtgtc gtgctttgtg
      661 aaccgggcgg aacgccggta tacggtaata ccggtgatct cgaggcccgt ctgcagaaga
      721 acgggagtgt tcgtttccgt gaccgtcgga cgggagagtt tgtctgtacg gattatggtg
      781 acagagttgt tttccgtaat caccatgacc gcaatgcgct ggcagataaa ctggatttga
      841 ttgcaccggt attattcggg cgtgatccac gcatggggtt tgaaccagaa ggaaacgaca
      901 aacaatttaa ccaggtcttt gctgaaatgg ttgcctggca taatgtgaca ggccgtaccg
      961 gtcacgaaga ctacaggatt acgcgaccgg acgtggatca tcaccgtgaa ggaagtgaac
     1021 gctactaccg cgattatatc gcggcgaaca gcaacgatga cgcatcactg cctccgccgg
     1081 agcaggataa acgctgggaa ccgccttctc caggttaaaa aaaaggccgc gccagaatcc
     1141 ggcgcggcca ctgttcagta cattccgtcc cagtccggac ggttggtttt tttatgatga
     1201 aacccgttta caggacgccc tgtcgggtgg ttattgtcct gtggaagcgt taacaggttt
     1261 atacctgctc tttctgctct cactgatatc tgattttcct cttttgtttc aggtagttct
     1321 ggtctgttca gcgtgatata gcgaattcgt cgctcttccc gtgtggtcgt tttaagcgta
     1381 ttcaccgctg tttcccacaa ggcactttca cgtgcgttga tatctgtgtc gtactcttcc
     1441 agcattgcca tagccgcctg acgtcctgca atgcgttgct gaaatgtatc cacgatcgtg
     1501 tgcggctctg tgcgaatttt tcgtcttttt ctggagggtt ttgcagtcag gacgccaatg
     1561 atggcactga cattttccgg tgacggtatt cgacgttgag tagtacgggg aacaagacgg
     1621 cggagacggt ccaggcgtgg cgcatccacg ctgatatacc tgtttataac aacgggatcg
     1681 ctgctgcagc ttttctcatc gtcagggatg aagaatgatg ctgatggtac ggtttctccc
     1741 cggaaaatag aaaagaactc gccaggatga agttcaatca actcctgaac atttatcttg
     1801 ctttccatca ggatgctgat ttgtgggctg tcaatccatg attcaccaaa tatgccgtcc
     1861 tgacgctgca ggttgttcat tctcgccctg gcttcttcac cggcggcgct tttcagcgtt
     1921 ctggccgtac taccctcgct gacaatcctg ccggcaaatt ttgtccccgt gttctgcatc
     1981 agtgttgcag tattcgtggc tgttgtctgt ccttctatac gttcctggtc ctgtgccatc
     2041 aaaatcagag caaaatccag tgaacgcacc tgagt
//
LOCUS       DAJOSY010000249         2013 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJOSY010000249 DAJOSY010000000
VERSION     DAJOSY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.251"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1566
                     /locus_tag="OHN27_004460"
     CDS             <1..1566
                     /locus_tag="OHN27_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCQ2288768.1"
                     /translation="ITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQG
                     RRAIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLT
                     FSRDEAGREVFRHSNRGFACEQGWNAASQLVSQRAGFFPEEATWDGLVPSLVREYRYD
                     SAGNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLK
                     AQSAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWD
                     SRDQLTGYCSAQGELWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDEL
                     YSVRHLVFNGFELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTV
                     WRPGQTSLWGLALSLPADTGYPDPRGELDPEADPGLLYAGQWRDAESGLCYNRFRYYE
                     PESGMYLVSDPLGLQGGEQTYRYVPNPLRWIDPLGLNKGASLSKMMNSSSDLMGLRRQ
                     PQNFWRLYRGKDI"
     gene            1599..1859
                     /locus_tag="OHN27_004461"
     CDS             1599..1859
                     /locus_tag="OHN27_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288769.1"
                     /translation="MLEHFGAEASVLDMTIIVRSNPSKAAILEEFLHGTQEKLGIAEK
                     LGRYGLGSAETHVKDFMIRHKKMLGLSDEDVAILKILKDKGL"
BASE COUNT          524 a          488 c          612 g          389 t
ORIGIN      
        1 atcacgccgg aaggcaaaac gcagtatgcc tatgacaaat ccggcaggct gacgggtatc
       61 ttcagcccgg acggtacatc acagcgcacc ggctatgacg aacgcgggcg ggtgaatgtc
      121 accactcagg gccgacgggc cattgaatac cactaccccg atgaacacac cgttatccgc
      181 tgtatcctgc caccggaaga tgaacgcgac agacaccccg atgaatccct gctgaaaacc
      241 acgtaccgtt ataacgccgc cggagaactg acggaggtca ttctgccggg ggatgagacg
      301 ctgacgttca gccgtgatga ggcgggacgt gaagtgttcc ggcacagtaa ccggggtttt
      361 gcctgtgagc agggctggaa tgcagccagc cagcttgtca gtcagcgcgc cggatttttt
      421 ccggaagaag ccacatggga cgggctggtt ccctcactgg tacgggagta ccgttacgac
      481 agcgcgggta acgtgtcagg tgtcaccagc cgggaagatt acggacggga aacccggcgg
      541 gaataccggc tggaccggaa cggccaggtc acggcggtga cagcctcagg caccgggctg
      601 ggctatggcg aaggcgacga gacttatggc tatgacagct gcggctacct gaaggcgcag
      661 tctgcgggca ggcaccggat aagtgaagag actgaccagt atgccggagg ccaccggctg
      721 aaacaggccg gaaacacgca gtatgactat gacgccgcag gccggatggt cagccggaca
      781 aaacaccgtg acggctaccg cccggaaaca gagcggttcc ggtgggacag ccgggaccag
      841 ctgaccgggt attgcagcgc acagggggag ctgtgggaat accgccacga cgccagcggc
      901 agacgaacag agaaacgctg cgaccggaag aaaatccgct tcacgtacct gtgggacggc
      961 gacagtattg cggaaatccg ggaataccgc gatgatgaac tgtacagcgt acggcacctg
     1021 gtgtttaacg gctttgagct gataagccag cagttcagcc gggtacggca gccgcatccg
     1081 tccgtggccc cgcagtgggt gacgcggacg aatcatgcgg tgagcgacct gacgggccgt
     1141 ccgctgatgc tctttaacag tgaaggtaaa accgtctggc gaccggggca gaccagcctg
     1201 tgggggctgg cactcagcct gcccgcagac accggctacc cggacccgcg cggggaactg
     1261 gacccggaag ccgaccccgg cctgctgtat gcggggcagt ggcgggatgc ggaatcgggg
     1321 ctgtgctata accggttccg gtattacgag ccggagagcg ggatgtacct ggtgagtgat
     1381 ccactggggt tgcagggagg ggagcagact taccggtatg tgccgaatcc tttaagatgg
     1441 atagatccct taggattaaa taaaggagct tcattatcta aaatgatgaa tagctccagt
     1501 gatctcatgg ggttgagaag gcagccccag aacttctggc ggctatatcg cggaaaagac
     1561 atttaataat agcattacca ggctcagatg aattcagaat gcttgaacat tttggagccg
     1621 aagcttcggt attagacatg acgataattg ttcgttcaaa tccgagtaaa gccgctattc
     1681 ttgaagagtt tttacatggt acacaagaga aactaggaat agctgaaaaa ttaggacgtt
     1741 atggactagg gagtgcagaa acgcacgtta aggatttcat gataagacac aaaaaaatgc
     1801 ttgggttatc tgatgaggat gtagcaatac tcaaaatatt gaaagacaag gggttgtgat
     1861 gaatataatt ctgccgataa aagatatatt tattattgcg ggaaacatag tttttagcgt
     1921 tttatacaat gaacatcttg agttcccatg ccgctgccaa ttactatctt caaattctga
     1981 agttatgcaa gagttatata ttgaaaaaga gtt
//
LOCUS       DAJOSY010000250         2004 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJOSY010000250 DAJOSY010000000
VERSION     DAJOSY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.252"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..522
                     /locus_tag="OHN27_004462"
     CDS             <1..522
                     /locus_tag="OHN27_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288770.1"
                     /translation="GSQWQSLPVLPADGTQVSFPYAGEWLAEDEIRVVLDAVRDAVCS
                     VSCRVAEDARRIRAALTTTGQTLLTRQTRRFRLVVKESDHPCWLDEDDENLPVVLDAI
                     LNRGARFSAVEMYLVSDCIEHILSSGLACDVLRIPDEPPRRWFDRGVLREVVREARAE
                     IRSMADALAKIRG"
     gene            1058..1360
                     /locus_tag="OHN27_004463"
     CDS             1058..1360
                     /locus_tag="OHN27_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCQ2288771.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGANWFDRTVSA
                     DAAGIIVTSLVINRQLWLYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            1407..1829
                     /locus_tag="OHN27_004464"
     CDS             1407..1829
                     /locus_tag="OHN27_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCQ2288772.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRQVTVPAVMLEKVLALAGSEM
                     KRLYAVGSENGGDGDVFVREEREAMDVVLQALDGEHMS"
     gene            1826..>2004
                     /locus_tag="OHN27_004465"
     CDS             1826..>2004
                     /locus_tag="OHN27_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288773.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQG"
BASE COUNT          401 a          523 c          635 g          445 t
ORIGIN      
        1 ggcagtcagt ggcagtcgct gccggtactt ccggcagacg gaacgcaggt gtctttcccg
       61 tatgccggtg agtggctggc agaggacgaa atcagggtag ttcttgatgc ggtgcgcgat
      121 gcagtatgca gtgtcagttg ccgggtggca gaagatgcgc ggcgtatccg ggcggcgctg
      181 accacaaccg gtcagacgtt actgacccgc cagacgcgcc gctttcgcct ggtcgtgaag
      241 gaaagcgatc acccctgctg gctcgatgag gacgacgaaa acctgcccgt ggtgcttgac
      301 gccatcctga accggggcgc acgtttttcg gcggtggaaa tgtatctggt cagcgattgt
      361 attgaacata tcctgtccag tgggctggcc tgcgatgtgc tgcgtatacc tgatgaaccg
      421 ccccgccggt ggtttgaccg tggtgttctg cgggaagttg tccgggaagc ccgggccgaa
      481 atccgcagca tggcggatgc cctggcaaaa atcaggggct gatgatggcg gggaaaatcc
      541 ccgctttttt tctgccggtc cccggacaga gcacaaagga agtcgccgcc ggctccgctt
      601 tacccggcca tgcaggcatg gccttgtggg ttttcagtta tgtggccacc gtgtcgtgtg
      661 cgggctgtgc cgtgcctcca tcttagccgg gctggcgggg atgcaagggt aaaatgaacg
      721 ctgcggtcac ccggtccctc cttcccgtct gccgtgattt tccggtcgtt cacccgctca
      781 ggtttcgcgg tctcaccctc caactcccgc cagccgtgtg cgtcaggctg cggcttccct
      841 tgcgccctgt gcatccccgc cttatcgccc ggcttttatg gaggcacggc accgcccggt
      901 gtcgcggaat atgtgaacaa cggaggaaaa catcatgcaa tatgcgaaac ctgtcactct
      961 gaatgttgaa gagtgcgacc gtctctcctt tctgccttac ctgtttggcc tggattttct
     1021 gtatgccgag gcatctgtgt acgcgctggc gaaaaaaatg atgccggaat atgaaggcgg
     1081 attctggcac ttcatccgcc tgccggacgg tggcggttac atgatgccgg atggcgaccg
     1141 ttttcatctg gtcaacggtg caaactggtt tgaccgtacg gtcagcgccg atgccgcagg
     1201 catcattgtg acttcccttg tgattaaccg tcagttgtgg ctgtaccacg acagcggtga
     1261 tgcaggactg actcacctgt acaggatgcg cgatgcgcag ttgtggagcc acatcgaatt
     1321 tcaccccgaa tgtaacgcga tttacgcggc gctggactga ttaacggacg gggcggcaat
     1381 gccgcccctg taaaggagat gagaacatgt actgtactgt taaagaaatt atccgcgaag
     1441 tgctggatac agacgtgccg gacagtgaat gcgtttttgc cgtggtgctg acccgtgggg
     1501 atgtgcgtca catagcccag gactggagtc tgacagacga tgagctggaa actgtcatgc
     1561 agcggctgga cgatgccttt gagcatggtg cggatgtcag cgttgttcac gacgttgttc
     1621 gtgaactgat ggaagaaaag cgcgccagcc gtcaggtgac agtcccggcg gtgatgctgg
     1681 aaaaagtgtt ggcgctggca ggcagtgaaa tgaagcgcct gtatgccgtc gggagtgaga
     1741 acgggggcga cggtgacgta ttcgtcaggg aagaacgcga agcaatggac gttgtgttac
     1801 aggcgctgga cggggagcac atgtcatgaa tatcagcaca gaaacccgcg aaattctgcg
     1861 caattacaaa gccgtgatta atgcgcggcg tcgtgaaatg gggcagaaac cgcttaccac
     1921 cgcgcagatt gttgatgaaa tctgcgattt tgtggtgaat cagcaggctg ttttcctcgg
     1981 cggtcactac atcttacagg gcag
//
LOCUS       DAJOSY010000251         1993 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJOSY010000251 DAJOSY010000000
VERSION     DAJOSY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.253"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            55..295
                     /locus_tag="OHN27_004466"
                     /pseudo
     CDS             55..295
                     /locus_tag="OHN27_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            387..602
                     /gene="sctI"
                     /locus_tag="OHN27_004467"
     CDS             387..602
                     /gene="sctI"
                     /locus_tag="OHN27_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HCQ2288774.1"
                     /translation="MIDLNDRVLNLDNPDDKMISAFANYAVQTENWQQNALQALRSDK
                     EGLTPEKLLVLQDHVLNYNVEVSLVEH"
     gene            643..1377
                     /locus_tag="OHN27_004468"
     CDS             643..1377
                     /locus_tag="OHN27_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HCQ2288775.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSFASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            1393..>1993
                     /locus_tag="OHN27_004469"
     CDS             1393..>1993
                     /locus_tag="OHN27_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCQ2288776.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMILLQ
                     YNLSVEHFNLNSSLVYCINNWNLFPLICLFSGCHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVEINEKARYKPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKIEMSLIKSPANGVVIKRKISDG"
BASE COUNT          649 a          338 c          387 g          619 t
ORIGIN      
        1 tcgagaagtc tcaaggtagc aactggacat ttcaggaata attgaggagg acttatggca
       61 gattggaatg gttatattat ggatatcagc aatcaatttg atcagggcgt tgatgatctg
      121 aaccagcaag ttgaaaaagc gttggaggat ttagccaacc aatccctccg acacgaaatt
      181 tcttgccgaa tatcaaagtg cattagctga gtatacatta tatcgaaatg cgcaatctaa
      241 cgttgtaaaa gcgtataaag atcttgattc agcaatcata caaaacttcc gctaaactgg
      301 ataaggattt tattatgtca gtaagcaata tgccacctat tgatagagct gagtaatcaa
      361 ctgctcatga aatacagcaa gcgaaagtga ttgatttaaa tgaccgggtt ctcaatcttg
      421 acaacccgga tgataaaatg atctccgctt ttgccaacta tgctgtacaa actgaaaatt
      481 ggcagcaaaa tgcgttacaa gcattaaggt ctgataaaga ggggttaact cctgaaaaat
      541 tattagtctt gcaggatcat gtgcttaatt ataatgtcga ggtttcattg gtggaacatt
      601 agcaagaaag attgtcgctg cagttgggac gttgacccgc tcatgaaata tatatcatta
      661 ttattgttta tcctgctgtt gtgcggctgc aagcagcagg aattattgaa ccatcttgat
      721 caacagcaag ctaatgatgt ccttgctgtt ttacaaagac ataatattaa tgctgaaaag
      781 aaggatcaag gcaaaacagg attttcaata tttgtcgaac caacggattt tgcttctgcg
      841 gtggattggc taaaaatcta caatctaccg ggtaagcctg atattcaaat atcgcagatg
      901 ttccctgcag atgcactcgt ttcatctcca cgagcggaaa aagcaaggct atattctgct
      961 attgagcagc ggttagagca atctctcaaa attatggatg gcattatttc ttcgagagta
     1021 catgttagtt acgatgttga taatggcgat agtggtaaaa ctgctttacc aattcatatt
     1081 tctgtacttg cagtatatga aaaagatatc aacccagaaa taaaaataaa tgatatcaag
     1141 agatttattg ttaatagttt tgcaagcgta caatatgaaa atatttctgt tgtattgtca
     1201 aaacgaagag acattattga gcaagcacca acatatgaaa ttagcgaacc tgtttttgct
     1261 tatgataagg ctatgccggt ttctatctta cttgcattaa tatccgtagc gacttgctgg
     1321 ctgttatgga agtatagggc cattctaact aatttagtca ggttgaaaat taagtagaca
     1381 tatgagagcg ttatgaatct tgccctaaga aagattatat atgatccaat tagttatatt
     1441 catcctcaac gagtaagctt gaataataca cctattaaca atccggtgct tagatctatt
     1501 accaatgaaa tgattcttct gcagtacaat ctttcagttg aacattttaa tctgaatagt
     1561 tcacttgttt actgcataaa caattggaat ttatttccat tgatctgttt attcagtggt
     1621 tgtcactttt atcgagaaag gtttgcagaa cgcggatttt tttataaggt tcctgctgtg
     1681 ctaagggatt atttatcagc tataccggtg gaaataaatg aaaaggcacg atacaaaccc
     1741 ggcatcgcta actatcacaa tatcatcaca tgtggttttt caacgttgtt accctatata
     1801 aggcaacaac cattggcaat gcagcaacga tttaatttat tatttcctga ttttgttgac
     1861 cacatacaat tgccgttacc acttgcatca actcttttag agagaattac attttatgcg
     1921 aaaaaaatag agatgagctt gataaaatct cctgcaaatg gtgttgtgat taaaagaaaa
     1981 atttctgatg gat
//
LOCUS       DAJOSY010000252         1969 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJOSY010000252 DAJOSY010000000
VERSION     DAJOSY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.254"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            134..1486
                     /locus_tag="OHN27_004470"
                     /pseudo
     CDS             134..1486
                     /locus_tag="OHN27_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1499..>1969
                     /locus_tag="OHN27_004471"
     CDS             1499..>1969
                     /locus_tag="OHN27_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCQ2288777.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFEYDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGN"
BASE COUNT          638 a          389 c          366 g          576 t
ORIGIN      
        1 ctgaaacatc ctactctctc actctaataa tggcaccttg ctcttcttat aaagaaggga
       61 ataattaaga atattaaact ttcatcaact tcacatcaag aaattacaaa acttaaacat
      121 taatctaaat aataacaacc tgatcacata atcgggtacg attttatccg ataactataa
      181 tgctataact ctgggaaatg gttggaatac accgctgggt gccatctctt ttgacgctac
      241 ccgatcgagt agtaagttaa ataatgatac cagacacgaa ggtacgagct atcaggtcgc
      301 ctataataaa tatctattgc aaacggccac tcactttagc gtggccgcct ggcgttatgc
      361 ttcgcaggat tataggacat tcagcgacca tctttacgaa aatgataaaa taaatcatca
      421 gagtgactat gacgattttt ataatattgg cagaaaaaat atcctttctg ccaatattat
      481 gcaaccttta tctaataatc tgggaaatgt atcattaagt gcactttggc ggaattattt
      541 ggggcgaagt ggaaatgcta aagattacca attcagttat tccaatagct ggcagcgcat
      601 cagttatact ttctctgcca gccaatccta tgatgaaaac gataaagaag aggagcgttt
      661 taatctgttc atctccattc ctttctactg gggggatgat attgccaaaa cacgtcacca
      721 aattaactta tcgaattcga cctcattttc caaagatggc tattcctcca acaatactgg
      781 aattactggc atagccggtg aacatgatca gttaaattat ggtatatatg ttaatcagca
      841 acaacaaaat aatgatacct cgcttggtac gaatttaagc tggaaaactc ccatcgccat
      901 aatagatggc agctatagcc attctaaaaa cgcctggcaa agtggtggaa gtattagtag
      961 tggattagtt gtctggcccg gtggtattaa tatcactaac cagttatccg atacatttgc
     1021 aattctggat gcgcctgggt tagaaggtgc gaatattaat ggacaaaaat acaaccgaac
     1081 aaatagcaaa ggccaggttg tttacgacct gattatacct catcgtgaaa accatctggt
     1141 acttgatata gcaaacagtg aaagtgaaac cgaattgcag ggcaatcgtc aaattattgc
     1201 gccttaccgt ggagcagttt cttatgtgca gtttacaact gaccaacgta agccctggta
     1261 tatacaggca ctgcgtcccg atggttcgcc attaaccttt ggctatgacg tactggattt
     1321 ccaggaaaac aatattggag tcgttggcca gggtagtcgc ctttttattc gcgtagatga
     1381 aattcccact ggcataaaag ttgctctcaa tgatgaacag aacttattct gtactattac
     1441 ttttcaacac gttatcgatg aaaacaaaac atatatatgc cagtaaaagg agtttactat
     1501 gaagtggctt ttattaatca ccttgagtct atattctttt attgtgcaaa gtgctccctg
     1561 tgcattgacc aatgtcggtg aacaacgtgg aacatatata ctcaagccac tgagtatgaa
     1621 aggtaatcta accgccgaaa aaattgaaaa caccggaaat atcattgagt gtacagactt
     1681 gcaagagaca accaacgcca ttaaagttca ttttttaaca cagaacgctt taaggtttaa
     1741 agtcggaagt cgtcattata ttattaattt cccctttgaa tatgacggta aaaaaataga
     1801 gcttccaggg aagtcatctt ataatttaga agaaatgcta tctgcgataa atttcaccat
     1861 tccatattct atcgcatctg tatcagatag ttctaacacc gcagacattt ttccaggaac
     1921 tccttttcct ttgttgttcg aaattgaagt gagttcatgt acagggaat
//
LOCUS       DAJOSY010000253         1962 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJOSY010000253 DAJOSY010000000
VERSION     DAJOSY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.255"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            37..540
                     /locus_tag="OHN27_004472"
     CDS             37..540
                     /locus_tag="OHN27_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HCQ2288778.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLYELRRISGCV
                     SVREWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            572..1303
                     /locus_tag="OHN27_004473"
     CDS             572..1303
                     /locus_tag="OHN27_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HCQ2288779.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            1556..>1962
                     /locus_tag="OHN27_004474"
     CDS             1556..>1962
                     /locus_tag="OHN27_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCQ2288780.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGR"
BASE COUNT          520 a          372 c          509 g          561 t
ORIGIN      
        1 tcaggataag cacaagcaat aatattgtat ggggagatga taatgaaaaa tttagctcat
       61 attgctctgg tgacggtgat acagttcatt gcctgttatc tggctgattg ggggagtgcc
      121 gaaacagttt ttatcctgtg ctttattgta ctatggcagg gattgtttat atggttattt
      181 tctcaaatac gaaaaaaacg gcatgtgtcg gatgagttta aattcagtaa aggcgtttgg
      241 tatatactga tgccagtatc ttccttactc tctccgttat taagcctgat ggtttttatc
      301 attggtacac tatacgaatt aaggcgaatt agtggttgtg taagtgtaag agaatggatg
      361 caatctcagg taaatgaaca aactaatgaa gatttgcatc tggattttga tgatgtgaat
      421 tttgattatg tggattttta tcgtactaat ccggcaacag gattagctat gcatggagga
      481 tttgattcag caggaaatac ttttgggact tgttggcagg actatgatga caggcgataa
      541 gaaaactgtg gtttgtaata aaggtaattt gatgaaaaca aaaaaattga tgatggttgc
      601 actggtcagt tccactctgg ccctttcagg gtgtggtgcg atgagcacag caatcaagaa
      661 gcgtaacctt gaggtgaaga ctcagatgag tgagaccatc tggcttgaac ccgccagcga
      721 acgcacggta tttctgcaga tcaaaaacac gtctgataaa gacatgagtg ggctgcaggg
      781 caaaattgct gatgctgtga aagcaaaagg atatcaggtg gtgacttctc cggataaagc
      841 ctactactgg attcaggcga atgtgctgaa ggccgataag atggatctgc gggaatctca
      901 gggatggctg aaccgtggtt atgaaggcgc agcagttggt gcagcgttag gtgccggtat
      961 caccggatat aattccagtt ctgccggtgc cacactcggt gtgggccttg ctgccggtct
     1021 ggtgggtatg gctgcagatg cgatggtgga agatgtgaac tataccatga tcacggatgt
     1081 gcagattgca gagcgtacta aggcaacggt gacaacggat aatgttgccg ccctgcgtca
     1141 gggcacatca ggtgcgaaaa ttcagaccag tactgaaaca ggtaaccagc ataaatacca
     1201 gacccgtgtg gtctcaaatg caaacaaggt taacctgaaa tttgaagagg cgaagcctgt
     1261 tctcgaagac cagctggcca aatcaattgc aaatattctc tgattatctt ccggaggctg
     1321 gtctgaccgg cctcctgact gacgccttgc tttgctcgtt gtccaacccc ggaaagaaat
     1381 aaaataaatt ttatttctcc ccggcgtttc cgaaataaat atgaaaacac tgagcggttg
     1441 tgcaaattct ttgtgcgacc gtgaagtgtt gccggcgaag ccggaattta acggttatta
     1501 atcttttcat cattaataat cagtccgact gactttcttt cttcggaata tcatcatgag
     1561 ttttaacgca aaggatatga cccagggcgg tcagattgcg tccatgcgta tccgcatgtt
     1621 cagccagatc gccaatatca tgctttactg cctgtttatt tttttctgga tactcgttgg
     1681 tctggtttta tgggtaaaaa taagctggca gacgtttgtg aacggctgta tttactggtg
     1741 gtgtaccacg ctggaaggta tgcgggattt aatcaggtcc cagccggtct atgagatcca
     1801 gtattacggc aaaaccttcc ggatgaacgc tgctcaggta ctgcatgata aatatatgat
     1861 ctggtgcgga gagcagctat ggtccgcatt cgttctggcc acagttgtgg cactggttat
     1921 ttgcctgatt accttctttg ttgtctcctg gattctgggg cg
//
LOCUS       DAJOSY010000254         1911 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJOSY010000254 DAJOSY010000000
VERSION     DAJOSY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.256"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..861
                     /locus_tag="OHN27_004475"
     CDS             <1..861
                     /locus_tag="OHN27_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HCQ2288781.1"
                     /translation="VEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISKEGATEWCAE
                     YDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKG
                     GWNLYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDA
                     LNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYH
                     THGADSHGEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGKEITNRKGL
                     PNVCRVHGNM"
     gene            833..1216
                     /locus_tag="OHN27_004476"
     CDS             833..1216
                     /locus_tag="OHN27_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbC/YhhH family protein"
                     /protein_id="HCQ2288782.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            1321..1671
                     /locus_tag="OHN27_004477"
                     /pseudo
     CDS             1321..1671
                     /locus_tag="OHN27_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
BASE COUNT          660 a          317 c          427 g          507 t
ORIGIN      
        1 gtggagcaga tgcaaaacca gatggacccg gtgtacacgc cggcgcgaaa aatccacctg
       61 taccactgcg accatcgcgg cctgccgctg gcccttatca gtaaggaagg ggcaacagaa
      121 tggtgcgcag aatacgatga gtggggcaac ctgctgaatg aagagaaccc gcatcagctg
      181 cagcagctta tccgcctgcc ggggcagcag tatgatgagg agtccggcct gtattacaac
      241 cgccaccgct attatgaccc gctgcagggg cgatatatca ctcaggatcc gattggactg
      301 aaggggggat ggaacctgta tggatatcaa ttgaatccga tatcagacat cgaccccctg
      361 ggtttatcta tgtgggagga tgcaaaatcg ggggcatgta ctaatggtct ttgcggcaca
      421 ctatccgcta tgataggtcc agataaattt gattctatag atagcaccgc atatgacgcc
      481 ttaaataaaa taaatagcca atctatttgc gaagataaag agttcgctgg tttaatatgt
      541 aaggataata gtggcagata tttctcaaca gcacctaacc gaggagaaag aaaaggatca
      601 tatccattca atagcccttg ccctaatggt actgagaaag tatcagctta tcatactcat
      661 ggtgcagata gtcatggaga atattgggac gaaatatttt caggtaaaga tgagaaaata
      721 gttaaaagta aagataacaa tatcaagtca ttttatttag gtacgcccag tggtaatttt
      781 aaagcaatag ataaccacgg gaaggaaata acaaacagaa aaggattacc taatgtctgc
      841 agagttcatg gtaatatgta aaaaaatatt gtttaggaac tgtgtcattg tatctttgtt
      901 tgtttttaca tacaacacat gggcgcagtg taataataat attaaaataa tgcgcaagta
      961 tgaaagtgaa ggtaaatata ccgttagaaa tttggttaaa aataaagcta tagcattgga
     1021 attagctgag atatatgtta agaatcgtta tggacaggat gccgcagaag aagaaaaacc
     1081 atacgaaatc actgagttaa caacaagttg ggttgttgaa ggtaccattc actcagacca
     1141 aattgctggt ggggttttta ttatagaaat aggcaaaaat gatgggagaa ttctgaattt
     1201 tggccacgga aaataagtat gtaaggataa tcaaacatcg tgtgcgctga tggcagagta
     1261 ggtggaggac tccagacagt caaacgatag aaaaagatag cctttatgga ggttcctgca
     1321 atgtcaaata cataccagaa aagaaaggca agtaaagagt atggtttata taataaatgt
     1381 aagaaactaa atgatgatga attatttcgc ttacttgatg atcgcaattc cttgaaaagg
     1441 atttcatctg ccagagtatt acagttaaga ggggggcaag acgctgttag attggcaatt
     1501 gaattctgca ctgacaaaaa ttatatccgt agagatatcg gagcatttat actcgggcaa
     1561 atataaattt gcaaaaaatg cgaagataat gtttttaata ttttgaacaa tatggcattg
     1621 aatgataaga gtgcttgcgt tcgagctacg gcaatcgagt caacggccag ggaaagatca
     1681 tcaaaagttc actttttgca ctaaataatt cgcattttat gtttaaaaat tgagatattc
     1741 cttattacct gaagctgttt tttattgctt atacatgatc aaatactcct tacctaatta
     1801 aggagaacaa aatggaactt aaaaaattga tggaacatat ttctattatc cccgattaca
     1861 gacaaacctg gaaagtggaa cataaattat cggatattct actgttgact a
//
LOCUS       DAJOSY010000255         1895 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJOSY010000255 DAJOSY010000000
VERSION     DAJOSY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.257"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            421..1796
                     /locus_tag="OHN27_004478"
                     /pseudo
     CDS             421..1796
                     /locus_tag="OHN27_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISEc13 family transposase"
BASE COUNT          464 a          489 c          508 g          434 t
ORIGIN      
        1 tatttgaaaa agatcacaaa attaattctc ctcatgtcca ctttgataaa aatataaaac
       61 tttgtgtcaa agacttgtta cgagatagag tcagacaata caatgacaat ggacattagt
      121 tttcgatgaa aggcagctcg caatcgcctc attcagtatg agacccgaac ttactcccac
      181 acgagtgtag ttatcgtctc ttcgtccact tttttcatca tcggtgagtt gctctatctt
      241 cccccccctt aaaggctgtc tcttatacac aaatccccac ccggggattt gtgatgtctg
      301 tcagtctgct ttagggggac tctttccatc cggtagtttc cttcctcccc ggcagcgatt
      361 ttttgaactt tttcctcacc cgttgatctt ctcttatatt tcattttctt cgggtctgtt
      421 atggtacttt ctgattgtta ttcatgggct aacgagcagt ttggacatgc ccgtctcggt
      481 gatccgcatc gtacacgacg ccttgtgtca ctggcctctt ctctcgctca gcatgcaggt
      541 ctttctattg tgaaatcatc ccagtccact gctcaggttg aaggtgcgta ccgcctgata
      601 cgtaacccgt ctgtgtcccc gcaagccatt gccgaagccg gatttaccgc cactgtaagg
      661 gcatgtgagg ctcatccgct gctcctcgcg ctggaagaca ccaccaccat taacttcagt
      721 cacagtacgg catctgatga cctggggaat accaccacta acccgaagac gcgcgggctg
      781 ctggctcact ctgtcctgat gtacgccccc gacagcgccc tgccagtcgg tcttatcgaa
      841 cagcagcgct ggagtcgtgc cacggacacc tacggtgtta agcaccagcg aaaagaacgt
      901 ccctatgaag aaaaagaaag ttaccgctgg cagcaggctt cggagcgcat ggccgaacgg
      961 ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga tatctggcat
     1021 tacctgcatt ataaagtcag ccacgggcag cgcttcgtgg tgcgtgcagc acagaatcgc
     1081 cggctggaag aagcacccgg caagctcttc gaactgccgg aagtcctagc aaccgcggaa
     1141 agccacacgc tgaatgtgat gcaaaaaggc gggcgggcag cccgccaggc ccggatgttc
     1201 atcagttaca gcgaagtcag cataaaaaat cccgacaaca gcggccaggc gctcccgctc
     1261 acgtatgtct gttgccggga gcaggcagag gacggcgcct gctggcatct gctgacgtca
     1321 gaaaaagtgg cgagtgccgc cgatgcgcga cgtatcgtca gccattccga gcgacgctgg
     1381 ctgatagagg aataccacaa ggcgtggaaa agtggtggta catgtgtgga atcgctgcga
     1441 atgcagaccc gggataacct cgagcgcatg gtggttatca aggcgtttat cgcggtgcgg
     1501 gtgctgggcc tgcggcaggg cgcggtatca gtgaagaaac acaaaatgac agctgtgaga
     1561 aaatactgac gccgacagag tggaaactgc tgtgggttaa gcgggaagga aaaccgcttc
     1621 cgtcacagat gccgacactg aaatgggcct gtctgaaact ggcgaaactg gggcgatggc
     1681 atgacagcaa acgcacaggt cgccccggtt gggtagtcat gtgggatggc tggttcagac
     1741 ttcaggatat ggttgagggt tacctggtga tgaagtctct tgatcaggag atctgatcaa
     1801 gagacagctt aaaggcgagg aagatatcaa acgctgtaaa taaaatgtaa gcgttaggtg
     1861 tagagcaatt ttctccggtc atagccacca acaga
//
LOCUS       DAJOSY010000256         1893 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJOSY010000256 DAJOSY010000000
VERSION     DAJOSY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.258"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(269..712)
                     /locus_tag="OHN27_004479"
     CDS             complement(269..712)
                     /locus_tag="OHN27_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCQ2288783.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRTELEDLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRVLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            869..1798
                     /gene="metA"
                     /locus_tag="OHN27_004480"
     CDS             869..1798
                     /gene="metA"
                     /locus_tag="OHN27_004480"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCQ2288784.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQDQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          457 a          516 c          443 g          477 t
ORIGIN      
        1 aaagactggg ccttttgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
       61 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
      121 cgcccgccat aaactgccag gcatcaaata aagcgaaagg ccatccgtca ggatggcctt
      181 tttgcattgg cgcagaaaaa aatgccggat gcgacgctgg cgcgtcttat ccagcttacg
      241 caggcacgat agcacgatag ggggcggctt attcccccac atacgccaga ttcagcaacg
      301 gatacggttt ccccaaatcg tccacctcag agcgtcccgt aaccttaaaa cccaccttct
      361 tatagaaccc aaccgcctgc tcattttgct cattaacgtt ggttgtcagt tccggtgcca
      421 tcgagagcgc atgctccacc agcacccgac ctacgccgca gccgcgcaca tcaggatcga
      481 taaacagcgc atccatatgc tgcccactta gcaacataaa tccaaccggc tgatcccgct
      541 cattaaccgc gacccacaac ggcgcttccg gcaggaagga acgaactagg tcctccagct
      601 cggtccgata ctctgctgat agaaaatcgt gagtggcatc gacagaacga caccaaatcg
      661 caacgagttc ctccccttcc tcatgccgtg agcggcgaat actaataacc attttctctc
      721 cttttagtca ttcttatatt ctaacgtagt cttttccttg aaactttctc accttcaaca
      781 tgcaggctcg acattggcaa attttctggt tatcttcagc tatctggatg tctaaacgta
      841 taagcgtatg tagtgaggta atcaggttat gccgattcgt gtgccggacg agctacccgc
      901 cgtcaatttc ttgcgtgaag aaaacgtctt tgtgatgaca acttctcgtg cgtctggtca
      961 ggaaattcgt ccacttaagg ttctgatcct taacctgatg ccgaagaaga ttgaaactga
     1021 aaatcagttt ctgcgcctgc tttcaaactc acctttgcag gtcgatattc agctgttgcg
     1081 catcgattcc cgtgaatcgc gcaacacgcc cgcagagcat ctgaacaact tctactgtaa
     1141 ctttgaagat attcaggatc agaactttga cggtttgatt gtaactggtg cgccgctggg
     1201 cctggtggag tttaatgatg tcgcttactg gccgcagatc aaacaggtgc tggagtggtc
     1261 gaaagatcac gtcacctcga cgctgtttgt ctgctgggcg gtacaggccg cgctcaatat
     1321 cctctacggc attcctaagc aaactcgcac cgacaaactc tctggcgttt acgagcatca
     1381 tattctccat cctcatgcgc ttctgacgcg tggctttgat gattcattcc tggcaccgca
     1441 ttcgcgctat gctgactttc cggcagcgtt gattcgtgat tacaccgatc tggaaattct
     1501 ggcagagacg gaagaagggg atgcatatct gtttgccagt aaagataagc gcattgcctt
     1561 tgtgacgggc catcccgaat atgatgcgca aacgctggcg caggaatttt tccgcgatgt
     1621 ggaagccgga ctagacccgg atgtaccgta taactatttc ccgcacaatg atccgcaaaa
     1681 tacaccgcga gcgagctggc gtagtcacgg taatttactg tttaccaact ggctcaacta
     1741 ttacgtctac cagatcacgc catacgatct acggcacatg aatccaacgc tggattaatc
     1801 ttctgtgata gtcgatcgtt aagcgattca gcaccttacc tcaggcacct tcgggtgcct
     1861 tttttatttc cgaaacgtac ctcagcaggt gaa
//
LOCUS       DAJOSY010000257         1889 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJOSY010000257 DAJOSY010000000
VERSION     DAJOSY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.259"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     repeat_region   1..1798
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..27
                     /rpt_unit_seq="gttccccgcgccagcggggataaaccg"
BASE COUNT          453 a          542 c          539 g          355 t
ORIGIN      
        1 gttccccgcg ccagcgggga taaaccgctg gcaatggatc gtatgaatct tgcgatagag
       61 tgttccccgc gccagcgggg ataaaccgcc tcgctgtaaa ttccaaaaac gatctctata
      121 gtgttccccg cgccagcggg gataaaccga aaataaatga taatcagacg gttagcaacg
      181 tgtgttcccc gcgccagcgg ggataaaccg gtgcgccagc tataaaaaac tcaccatcaa
      241 cagtgttccc cgcgccagcg gggataaacc gcatttttgc gtgacgagct gcgccgcgtt
      301 ctggtgttcc ccgcgccagc ggggataaac cgcgccacca gcgatagagc cgcaaacaga
      361 gccggtgttc cccgcgccag cggggataaa ccggatatgg caatgagtaa atatacatat
      421 ttaaacgtgt tccccgcgcc agcggggata aaccgatccc ctgcgtcctc ttttgaataa
      481 tcgcggcgtg ttccccgcgc cagcggggat aaaccgcgag cgccgttggc tactggcgta
      541 gccgtggagt gttccccgcg ccagcgggga taaaccgcca tgactataaa agcttatatt
      601 ttgataaatg tgttccccgc gccagcgggg ataaaccggc gtctcgagcg ccggacgatt
      661 caaaaccagc gtgttccccg cgccagcggg gataaaccca ggtcctcgtc agatacagta
      721 gtcacaacac cgtgttcccc gcgccagcgg ggataaaccg cactgacagt gaagctgaat
      781 tacaggcaga gggtgttccc cgcgccagcg gggataaacc ggcgtccgtc aaagaggaag
      841 ccaaagccat gcagtgttcc ccgcgccagc ggggataaac cggcgaccac gcagagcggc
      901 gacaacctga cgttgtgttc cccgcgccag cggggataaa ccgctatagc agcactccac
      961 cagctcgagc agcaggtgtt ccccgcgcca gcggggataa accgcaaaaa aataatatcc
     1021 ggcagtctgt acggtagtgt tccccgcgcc agcggggata aaccgtggca ggccggtaat
     1081 atgaaaacga cagagcagtg ttccccgcgc cagcggggat aaaccgtcgg tttgcgcgta
     1141 ttgttgttcg gggtctatgt gttccccgcg ccagcgggga taaaccgtag gtaaatcaca
     1201 gctatttgat aagggcgtgt gtgttccccg cgccagcggg gataaaccgc agttgaagcg
     1261 ctttaaaaaa ttctggacgg cgtgttcccc gcgccagcgg ggataaaccg agatcgacat
     1321 gagccgccgc gcgacgaata acgtgttccc cgcgccagcg gggataaacc gtaccgataa
     1381 tcggtacaag atagtattta catgtgttcc ccgcgccagc ggggataaac cggactatcg
     1441 cgggctgctg aaatcaaccg gcgagtgttc cccgcgccag cggggataaa ccgagtcgaa
     1501 atgaggcgcg tgttctggag gatatgtgtt ccccgcgcca gcggggataa acctgtgttt
     1561 gcggcattaa cgctcaccag catttcgtgt tccccgcgcc agcggggata aaccgacgtg
     1621 gtcatgggtg ctgctgttgc agagccagtg ttccccgcgc cagcggggat aaaccgaata
     1681 gcaatagtcc atagatttgc gaaaacaggt gttccccgcg ccagcgggga taaaccggag
     1741 cctgacgaga ctactgaggc cgttctgtcg agttccccgc gccagcgggg ataaaccacc
     1801 atataacccg ttatctcttt ctcaagtttt tatattagca gtacttgtaa taagcaacat
     1861 atccacgtaa cacctcatgt tcaaaatag
//
LOCUS       DAJOSY010000258         1854 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJOSY010000258 DAJOSY010000000
VERSION     DAJOSY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.260"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..63
                     /locus_tag="OHN27_004481"
     CDS             <1..63
                     /locus_tag="OHN27_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HCQ2288785.1"
                     /translation="IALCEWDKHYFKLQSCVRES"
     gene            65..358
                     /locus_tag="OHN27_004482"
     CDS             65..358
                     /locus_tag="OHN27_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCQ2288786.1"
                     /translation="MSLVGYNSKIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISTAELEQKLYREFAQVTTVPAYKEMSMYQLLVISQDHLTH"
     gene            complement(420..782)
                     /locus_tag="OHN27_004483"
     CDS             complement(420..782)
                     /locus_tag="OHN27_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCQ2288787.1"
                     /translation="MIANPTHIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIG
                     IPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLWLEDVENAGQPVPDEQLSSEDK
                     FIEGEEKEIENKDDNLKN"
     gene            complement(809..1540)
                     /locus_tag="OHN27_004484"
     CDS             complement(809..1540)
                     /locus_tag="OHN27_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCQ2288788.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKK
                     QLATEAVKFKLDSLNPVNGLKRIFGLKTVKESVKAILYIIFFALAIKVFWSNHKSLLF
                     KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKRE
                     YKEQEGNPEIKSKRRERIRKFFLSN"
     gene            complement(1549..1785)
                     /locus_tag="OHN27_004485"
     CDS             complement(1549..1785)
                     /locus_tag="OHN27_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCQ2288789.1"
                     /translation="MTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTVKSA
                     LAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
BASE COUNT          614 a          335 c          310 g          595 t
ORIGIN      
        1 atagcactct gtgaatggga taaacattat tttaaactcc aatcttgcgt aagagaaagt
       61 tgatatgtca ttagtaggat ataatagtaa aatcataata accatcgcgc tgatatcgtt
      121 gctctctgca tgcaccagag aaaattcatt aaaggagaat caaaataaaa gaagtggcga
      181 atcccaagcg atagtagatg caagacagtt ctttagcgaa cacccggaat atatatctac
      241 agcagaactg gagcaaaagc tatatcgcga atttgcacag gttactacag ttccagctta
      301 taaagagatg agtatgtatc aattgcttgt catttcacaa gatcatttaa ctcattaaat
      361 aatgaatcta cacggttttt gcgtgtagat tcttataaat ttctgactac gacagatgtt
      421 taatttttta aattatcgtc tttattctcg atttctttct cttcaccttc aatgaattta
      481 tcttctgaag agagctgttc atctggaaca ggttgtccag cattctccac atcttcaagc
      541 cacagcagaa gacgtaatat ttcatctata ttttcgaagc taacataatc gtagcgacga
      601 tgggtagcat aaatttttcg tgctaatttt ttatctgtga taattggtat tccgatttcc
      661 tttgcatatt tccttacggc taaggctacc tcattagttt ctcttacaga aatcaatgga
      721 ataggtgaaa gatgtggctt aaagtatatc cctattgcaa tgtgagtagg gttggcaatc
      781 atcaaacgac tattactgac atcagacttc aattgctcag aaagaatttc ctgatgcgct
      841 ctctgcgttt agacttaatt tctggatttc cttcttgctc cttgtattct cttttaactt
      901 cttgtttatc cattttcata tctttcataa ataaaaaata ttcagcgata aaatcaaaaa
      961 ttaagacaat tatcatactg ccgagacaat acagtatgag aaggaatagc atctctcccc
     1021 aatctgataa taaagatatg atatctccat caagagtttt aaaaagcagt gatttatgat
     1081 tactccaaaa tactttgatc gccaatgcaa aaaaaataat ataaagaatt gctttaacag
     1141 attcttttac ggtttttaag ccaaatatac gctttaagcc atttactgga ttcaatgaat
     1201 caagcttaaa tttaacagct tcagttgcta attgcttttt actttgaaac caggagacaa
     1261 gtattgttgt gaacatgcaa accagcaaaa aaggaatgtt cgttttcaac caaccaaccc
     1321 ccatagcttt aaaatagtcc caaatatctg gtttagcgtt atgatcaagg atgtattcaa
     1381 gaatcgacat aatgtgatgc acatcaaaaa catatccgag atataaagta cccacaagca
     1441 ttattacaga gatggttaag tctctacttt ttagaatttg ccctttttta gaagcatcct
     1501 ggagtttctt ttgtgttggc ttttcagttt tattggccat gaaacactct atttctcaat
     1561 gtatagttgc aattgttctg ggaaaaagga taacggcatt actcgttcag ccagtattgg
     1621 gccaaaataa ataattaata ttaaaaatgc cagcgcactt ttgacagtca gtgaaatggc
     1681 aaaagcattt agttgtgaag cataacgtgc cagcaatccc aggacagcct caccacccag
     1741 cataacggca atcacggggc ttgcgtaaac gatagtatga gtcattatgt tattaatata
     1801 tgagaataaa ggctctaatt taggaaaatt aaaattgcct gaaggccata aatt
//
LOCUS       DAJOSY010000259         1797 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJOSY010000259 DAJOSY010000000
VERSION     DAJOSY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.261"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            5..1167
                     /locus_tag="OHN27_004486"
     CDS             join(5..266,266..1167)
                     /locus_tag="OHN27_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HCQ2288790.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            1200..1628
                     /locus_tag="OHN27_004487"
                     /pseudo
     CDS             1200..1628
                     /locus_tag="OHN27_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1615..>1797
                     /locus_tag="OHN27_004488"
     CDS             1615..>1797
                     /locus_tag="OHN27_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288791.1"
                     /translation="MDIIRNSVWLSQGTDLLAEGLYRVLDFDRKVDLLILFKIKSERT
                     GKPIPFSFSMFKYYIES"
BASE COUNT          499 a          399 c          457 g          442 t
ORIGIN      
        1 tcacatgaca aaaacagtat caaccagtaa aaaaccccgt aaacagcatt cgcctgaatt
       61 tcgcagtgaa gccctgaagc ttgctgaacg catcggtgtt actgccgcag cccgtgaact
      121 cagcctgtat gaatcacaac tctacaactg gcgcagtaaa cagcaaaatc agcagacgtc
      181 ttctgaacgt gaactggaga tgtctaccga gattgcacgt ctcaaacgcc agctggcaga
      241 acgggatgaa gagctggcta tcctccaaaa ggccgcgaca tacttcgcga agcgcctgaa
      301 atgaagtatg tctttattga aaaacatcag gctgagttca gcatcaaagc aatgtgccgc
      361 gtgctccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac aaggataagc
      421 acgcgtcagc agttccgcca acactgcgac agcgttgtcc tcgcggcttt tacccggtca
      481 aaacagcgtt acggtgcccc acgcctgacg gatgaactgc gtgctcaggg ttaccccttt
      541 aacgtaaaaa ccgtggcggc aagcctgcgc cgtcagggac tgagggcaaa ggcctcccgg
      601 aagttcagcc cggtcagcta ccgcgcacac ggcctgcctg tgtcagaaaa tctgttggag
      661 caggattttt acgccagtgg cccgaaccag aagtgggcag gagacatcac gtacttacgt
      721 acagatgaag gctggctgta tctggcagtg gtcattgacc tgtggtcacg tgccgttatt
      781 ggctggtcaa tgtcgccacg catgacggcg caactggcct gcgatgccct gcagatggcg
      841 ctgtggcggc gtaagaggcc ccggaacgtt atcgttcaca cggaccgtgg aggccagtac
      901 tgttcagcag attatcaggc gcaactgaag cggcataatc tgcgtggaag tatgagcgca
      961 aaaggttgct gctacgataa tgcctgcgtg gaaagcttct ttcattcgct gaaagtggaa
     1021 tgtatccatg gagaacactt tatcagccgg gaaataatgc gggcaacggt gtttaattat
     1081 atcgaatgtg attacaatcg gtggcggcgg cacagttggt gtggcggcct cagtccggaa
     1141 caatttgaaa acaagaacct cgcttaggcc tgtgtccata ttacgtgggt aggatcacta
     1201 tagcagtaaa acataacgag agcatacctg aacgcgaagc ccaaaaactt gaaatagaga
     1261 ggatgttttg gcagttgatt gatgttgagt ttcaaattta tgttggctcg gaactcaata
     1321 acgtcgtcgg taaaaacatt tgctgggcta cttctgtatt aagagatggt tctgaatttt
     1381 atgataaata tcctcttgat aaaatcctat ggaagcttaa accagatgtt tatcccatag
     1441 taggactacg tgcaatgatt tcatcaatct ttggggtaga tgcacaagaa gcgatgatgt
     1501 tattgcaggc aatgattgga ttaaaaatga taaatgttga tttatcatat ccaatactcg
     1561 aaaccggtct gataaagata atttccaatg accactatat aggactgaac gcaaatggat
     1621 attattagga attcagtctg gctctctcaa ggcactgatt tgcttgcaga gggactttac
     1681 cgtgttttgg attttgacag aaaggtcgat ttgttaattt tgtttaaaat aaaatcagaa
     1741 agaacgggta agccaatccc tttctcattt tcaatgttta aatattatat tgaatca
//
LOCUS       DAJOSY010000260         1782 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJOSY010000260 DAJOSY010000000
VERSION     DAJOSY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.262"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            218..394
                     /locus_tag="OHN27_004489"
     CDS             218..394
                     /locus_tag="OHN27_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing PndC domain protein"
                     /protein_id="HCQ2288792.1"
                     /translation="MDSEAREYRSFREAIATESTEEVRFHYVFIDGYWKVMYRTPEGW
                     KMKALALALRRCPK"
     gene            488..754
                     /locus_tag="OHN27_004490"
     CDS             488..754
                     /locus_tag="OHN27_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288793.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            819..1757
                     /locus_tag="OHN27_004491"
     CDS             819..1757
                     /locus_tag="OHN27_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCQ2288794.1"
                     /translation="MSQKKTTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGRMEGAL
                     EKALAIACQLQKMGMTPEQIKQATGLSDDELKKITH"
BASE COUNT          426 a          482 c          512 g          362 t
ORIGIN      
        1 ggtgttccgt accgtttact gccacctgca cggggagccg acctggaacg gtcgcatcct
       61 gcatacccac tatgccaccg gtcagcaggc cgaagccctg gttgaacacg gtgatatccg
      121 ttgcctcggt ccccgttgcg acaaacccgc cgggcatacg cttcagaacc cggtggacgg
      181 tgtgacggct tattacggac gtgacagtgg tttccggatg gacagtgagg cgcgtgagta
      241 ccgttctttc agggaggcta ttgccactga aagcactgaa gaggtgcgct tccattatgt
      301 gttcatcgac ggctactgga aggtgatgta ccgcacgccg gaaggctgga agatgaaagc
      361 gctcgcgctg gcactgcgtc gctgtccgaa atgaaaaaaa ggcagggcga taaaccctgc
      421 ctctctccgc cggcgcttcc ccgccaggaa gtccggcatc tcaatcacta tctatggaga
      481 ttatgccatg agaccatcaa ttatcttcgc aaccgccgag tatgtaaagc gtctgcgtga
      541 agagtgcctg cgggagaata aacccctgca ccgccatacc cgcttcagac gtcaggagct
      601 ggcacaggat gagattaacc cggacgtcct ggcgatgagc ggccatatcg ccagacgctg
      661 cagtgagcag aagcgggtgc gtatccccgc tatgaaagtc agcgaatggg gccacctgct
      721 ccgcgcgctt gaaattgagc gggtctgcca ctgattaacc cttgccgtct gcctgtgcag
      781 gcggcaggat acgttcagac atctcaccag gaggaaccgt gagtcagaag aaaaccacca
      841 cgacgcccac gccgcatgat gccgcgttcc ggtcgttcct ggcgaatccc gacgtcgcca
      901 gagattttct ggaactgcat cttccggcgg agtaccggca gttgtgcgac ctgtccacgc
      961 tgaagctgga acccgccacc tttgttgagc cggacctgca tcagtacgcc agcgatatcc
     1021 tctggagtgt gaaaaccacc gggggtgaag atggctatgt ttatacgctc atcgagcacc
     1081 agagcaccga aaatctgtac atgcctttcc gcatgttacg ttacagtgtg gcggcgatgc
     1141 agagacatct ggagcagcac aaaacgttgc cactggtgat tcctgtactg ttctatcatg
     1201 gtgagcgcag cccgtacccg tacagcatga actggctgga ctgttttgag aatccggcac
     1261 ttgcggctaa aatatacaca aagccgtttc cgctggttga tatcactgtc gttgatgaca
     1321 atgaaatcat gaaccatcgc cggatggccg cactgacgct gctgatgaag catatccgcc
     1381 atcgtgacat gatggagctg ctggacaaac tcccgcaggt catggtggaa atttcagacg
     1441 agcaggtgcg tgttctgatt cattacatcg ttaacgcagg ggactctgta tcaccggaat
     1501 ttatgcgggc gctggctgag cgtctgccgc agcatgagga taaactgatg actatcgcag
     1561 aacgtcttga gcaaaaaggt cgccaggagg gcaggatgga aggacgcatg gaaggacgca
     1621 tggaaggacg catggaagga gcgcttgaaa aagccctggc tattgcgtgc cagcttcaga
     1681 aaatggggat gacgccggag cagattaagc aggctaccgg actttccgat gacgaactga
     1741 agaaaatcac tcactgagta aacatcacag cccggcaaaa tg
//
LOCUS       DAJOSY010000261         1765 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJOSY010000261 DAJOSY010000000
VERSION     DAJOSY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.263"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(61..822)
                     /gene="kduD"
                     /locus_tag="OHN27_004492"
     CDS             complement(61..822)
                     /gene="kduD"
                     /locus_tag="OHN27_004492"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCQ2288795.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(852..1688)
                     /gene="kduI"
                     /locus_tag="OHN27_004493"
     CDS             complement(852..1688)
                     /gene="kduI"
                     /locus_tag="OHN27_004493"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCQ2288796.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
BASE COUNT          460 a          477 c          396 g          432 t
ORIGIN      
        1 tgatctacag gtataaaaaa ccctgccatg cggcagggtc ataaaagtaa gaagaatgaa
       61 ttaacgcgcc agccaaccgc catccacggc aatggtataa ccattcacat aatctgaagc
      121 gctggaggca aggaacacta tcggccccat caggtcactc ggcagtcccc aacgaccagc
      181 tggaatgcgg tcgagaattt ccgcgctacg ttgttcatct gcccgtagtt gttgagtatt
      241 gttggtcgcc atgtaacccg gggctatcgc attaacatta atgttgtgtt tagcccattc
      301 gttcgccatc aatcgcgtca cacccatcac gccgcttttt gatgcggtat aagaaggcac
      361 acggatcccg ccctggaagg agagcattga cgcgatattg ataatcttgc cgccattgcc
      421 ttgcgcgata aagtgtttcg ccgctgcctg agacatgaag aatacgctct tgatattcag
      481 gttcatgaca tcgtcccagt ccttttcgct gaactcgaga gcatcttcgc ggcgaatcaa
      541 tccggcgtta ttcaccagga tatcaatatg accaaactcc gctaccgcgc gatccagcag
      601 tgctggaata ccatcaatct ttcgcagatc ggcggtcagg cttaaaaaac gacgccccag
      661 cgctgtgacc tgctcgatgg tttcagtcgg ttcaacgatg ttaatgccaa caatgtcaca
      721 gcccgcttgc gccagcccca acgccatccc ctgacccagt ccagtatcac aaccagtgac
      781 gaccgcaact ttaccttcga gagaaaatgc acttaaaatc ataacaatac cttattcgtt
      841 tatgcccaca actagcgcaa atctttaacg gccacatggt ccatatcatc aaagacctgg
      901 ttttcaccga ccatgcccca gataaaggta taagctttgg ttccgacacc ggaatggatc
      961 gaccagctcg gggagatcac cgcctgctcg ttatgcatca caatatgacg cgtttcttgc
     1021 ggctgcccca tcatgtggaa aacgcaggcg tcatcatcca tattgaaata gaaataaact
     1081 tccatccggc gctcgtgggt gtgacacggc atggtgttcc acaagttacc cggagccagc
     1141 tccgtcagcc ccatactcaa ttggcaggtt tccagtacat ccgggacaaa atatttgtta
     1201 atcgtgcgac ggttactggt gaggttatcg cctaacgtga ctggagatac ttcgtccggt
     1261 gtgacttttt tggtgggata cgtcgtatgc gcgggtgcgc aattgtaata aaacttcgcc
     1321 ggagtgccgg tatcaatact ggcaaagaca acttcttttg cacctttacc aacatacagg
     1381 gcgtcgcggt gaccgatttc atagcattgg ccatcgacag taatcgtacc ggcaccgcca
     1441 atattgataa cacctaactc gcgacgttca aggaaatagc ttacgccgag ttgtttacca
     1501 acttccccgc caacggaaac cgttttagtt atcggcataa tgccgccaac aataattcgg
     1561 tcaatgtggc tgtaaaccat ggtgtactca tcggcgacaa ataccttttc aaccaaaaat
     1621 tcattgcgca gcccttgggt atccagcgtt tttgcgtgcg cactgtggat gctctgtctt
     1681 acgtccacat caacctccga taatcagtga agcgtgaaaa tagaaaacga tagtgaaaca
     1741 ctgtttcgtt ttctctataa aggga
//
LOCUS       DAJOSY010000262         1709 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJOSY010000262 DAJOSY010000000
VERSION     DAJOSY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.264"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..343
                     /locus_tag="OHN27_004494"
     CDS             <1..343
                     /locus_tag="OHN27_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HCQ2288797.1"
                     /translation="MADHFEYEEDFETDDFDIKKNESEIYDENDDQMINSFEDLPVKI
                     EFVLGKKIMNLYEIDELCAKRIISLLSESEKNIEIRVNGALTGYGELVEVDDKLGVEI
                     HSWLSGHNNVK"
     gene            333..998
                     /locus_tag="OHN27_004495"
     CDS             333..998
                     /locus_tag="OHN27_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HCQ2288798.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVNFFEKIQKVNSSEDNEEIFDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            1008..1268
                     /locus_tag="OHN27_004496"
     CDS             1008..1268
                     /locus_tag="OHN27_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HCQ2288799.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            1270..>1709
                     /gene="sctT"
                     /locus_tag="OHN27_004497"
     CDS             1270..>1709
                     /gene="sctT"
                     /locus_tag="OHN27_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HCQ2288800.1"
                     /translation="MGEAILYQLHSLLAATALCFCRLAPTFYLLPFFASGNIPTVVHH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFIL"
BASE COUNT          498 a          255 c          366 g          590 t
ORIGIN      
        1 aatggctgat cattttgagt acgaggaaga ttttgaaaca gatgattttg atatcaaaaa
       61 aaacgagagt gagatttatg atgaaaatga cgatcagatg attaatagtt ttgaagactt
      121 gcctgtaaaa attgagtttg ttcttggtaa gaaaataatg aatctatatg aaatagacga
      181 actctgtgca aaaagaataa tatctctgct ttctgaatct gagaaaaata tagaaatacg
      241 tgtaaatggc gcgctaactg gctatggcga acttgttgaa gtggatgata aattgggcgt
      301 agagattcat tcttggttat cagggcataa taatgtcaaa tagcatttcg ctgatagcta
      361 tactatcatt atttacacta ttgccgttta taatagcatc cgggacatat tttattaaat
      421 tctcgattgt ttttgttatt gtccgaaacg cgctggggct tcagcaagtg ccatcaaata
      481 tgacacttaa tggggttgct ttgctattgt caatgtttgt aatgatgccg gttgggaagg
      541 aaatatatta taacagtcag aatgagaatc tttcattcaa taatgtagcg tcggttgtta
      601 attttgttga gacaggcatg agtggttata aatcatattt gataaaatat tccgaaccgg
      661 agttggttaa cttttttgaa aagattcaga aggtgaatag ctctgaagac aatgaagaga
      721 tattcgatga tgataatatt tcaattttct cgttactccc ggcatatgcg ttgagtgaga
      781 taaaaagtgc atttattata ggtttttata tctatttacc ctttgttgta gtggatctgg
      841 ttatatccag cgttctgtta acgttaggta tgatgatgat gagtccggta accatatcta
      901 caccgataaa attaattttg tttgtcgcga tggacggttg gacaatgtta tcaaaaggac
      961 tcattttaca atattttgac ctatccatta atccctgagg aaaaaatatg gacgatattg
     1021 tttttgcagg gaatagggcg ctgtatctga ttttagttat gtctgcagga cctatcgcag
     1081 ttgcgacatt tgttggttta ttagtagggc tttttcaaac ggtaacacag ttgcaagaac
     1141 agacgttgcc atttggcgtg aaattattat gcgtaagtat ttgctttttt ttaatgagcg
     1201 gatggtatgg cgaaaaatta tattcttttg gtattgaaat gctgaattta gcattcgcga
     1261 gaggatagca tgggtgaggc gatactctat cagttgcata gtctgctggc tgctacagcg
     1321 ctctgttttt gccgattggc acccactttc tatttgttac catttttcgc cagtgggaat
     1381 ataccaacag tagtccatca tccaataata atagtggtat catgtgcatt agttcagcat
     1441 taccactatg aattattgaa cttaaatgaa atcgatattg ctttgttcgc tgccagagaa
     1501 attatcatag ggctgtttat tgcatgctta ttagcatccc cgttttggat tttccttgct
     1561 ataggtagct ttatcgataa ccaacgcggt gcaacattaa gtagtacttt ggatccggct
     1621 acaggtgtcg atacctcgga attagcaaga ttatttaacc ttttctctgc tgcggtttat
     1681 cttactaaag gtggaatgaa ctttattct
//
LOCUS       DAJOSY010000263         1656 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJOSY010000263 DAJOSY010000000
VERSION     DAJOSY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.265"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            168..506
                     /locus_tag="OHN27_004498"
     CDS             168..506
                     /locus_tag="OHN27_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCQ2288801.1"
                     /translation="MTHKRIPKDWVIKRSTPFFTKENVPSALLTHHNTAAGVFGQLCV
                     MEGTVTYYGFTDENATEPEIKVVINAGAFATSPPQYWHRVELSDDAQFNINFWVAPDF
                     SGEKVYSTKK"
     gene            complement(1269..>1656)
                     /locus_tag="OHN27_004499"
     CDS             complement(1269..>1656)
                     /locus_tag="OHN27_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HCQ2288802.1"
                     /translation="NNKTQIIKFHGDFDDDSSIVLDETSYFQRLEFETPLDIKFRSDV
                     LGKSVLFIGYSLSDINIRLLFYKLSKLWKEQKLEEAQPKSYIFLPRPNPIQEEILEQW
                     RIGMISSENDNPGESLEEFLKNFVLV"
BASE COUNT          555 a          343 c          276 g          482 t
ORIGIN      
        1 taacacacag aatgcataaa ctaaaagtaa aacaaatagt tatacgaaaa acaaccatat
       61 ttattatatc ttctcctcta atcaacatat caattatgtg tttcataaca gcttgacata
      121 accctcaaaa acacgcattt ataatgcatg tttttgaggg ggtggttatg acacacaaga
      181 gaattcctaa agattgggta atcaaacgct caactccgtt cttcacaaaa gagaacgtac
      241 cttcagcgtt attaacacat cataatacag cagcaggtgt ttttgggcag ttgtgcgtaa
      301 tggaaggcac tgtaacatat tatggtttta ctgatgagaa tgctactgaa ccggagataa
      361 aagtagtcat taatgctggc gcttttgcaa caagcccacc acaatactgg caccgtgttg
      421 aactaagcga tgatgctcag tttaatatta acttttgggt cgctccagac ttctcgggcg
      481 aaaaagtcta tagcaccaaa aaatagatga tcatattaaa aatgctttaa cagtcagacc
      541 cggcagggct atccccgacg ggtctcattt ttgtaaatat attggttcta cattgacagg
      601 agacttcaac cataagaagt aatctgaata cttgagcaat aaatttactt aagattaatg
      661 agcgcacctt gccggacaaa gataaaccaa ggtaacacct tttttctagc cagtatgtaa
      721 tcaatcagaa agccgctcca taagaacaac agcaagacaa taaattgcca ttacagccgc
      781 aatagccagc gcacatttga gaaccagcac gacaaccccc tgtattagac gcacaccagt
      841 cctgataaat atgaggctgt ctcgtcagtg attcaataca actattgggt atagtttctg
      901 tgattttatt ctgtagaaat ggaacacaac aaccagtcac caccagcact tctttaaata
      961 cgccaagtcc gacgcaagct aaccttctag tccgctttga gcgaaaagcg gacaggaaca
     1021 ttctggttaa tagatatcaa tgctaacaaa aaagctcccg atggagattt tgaaaccgct
     1081 tccacataat gaaataaaag gatttatttc tggtcatgcc cacacattga ccacgtcgaa
     1141 gtaagtagct ccgcttcgcg gagttactta agataatttc tatgtagttc catgctatga
     1201 tttaattttc aagttacaaa atctaaatct acaaatgtcc atttgggtgt aaaattacac
     1261 aatcctatct atactaatac gaaattcttc aaaaactctt ccaaagactc gcctggatta
     1321 tcgttttctg atgaaatcat gcctattctc cactgttcca atatttcctc ttgaattggg
     1381 tttggtcgtg gtaagaaaat ataagacttt ggctgtgctt cttctaactt ttgctctttc
     1441 caaagcttgg acaatttata aaatagtagt ctgatattaa tatctgacag actataccca
     1501 ataaaaagaa ctgatttccc gagaacatca gatctaaact ttatatccaa tggagtttca
     1561 aactctaacc gttgaaagta acttgtttca tctaatacaa ttgaactatc gtcatcaaag
     1621 tctccatgaa acttgattat ttgcgtctta ttatta
//
LOCUS       DAJOSY010000264         1648 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJOSY010000264 DAJOSY010000000
VERSION     DAJOSY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.266"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            61..1227
                     /gene="ugd"
                     /locus_tag="OHN27_004500"
     CDS             61..1227
                     /gene="ugd"
                     /locus_tag="OHN27_004500"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCQ2288803.1"
                     /translation="MKITISGTGYVGLSNGVLIAQNHEVVALDIVQAKVDMLNQKISP
                     IVDKEIQEYLAEKPLNFRATTDKHDAYRNADYVIIATPTDYDPKTNYFNTSTVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTRDIKERLGIDNVIFSPEFLREGRALYDNLHPSRIVI
                     GERSARAERFADLLKEGAIKQDIPTLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESQGLNSKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIAAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGIPVII
                     YEPVMQEDEFFNSRVVRDLDAFKQEADVIISNRMAEELADVADKVYTRDLFGND"
     gene            complement(1515..>1648)
                     /locus_tag="OHN27_004501"
     CDS             complement(1515..>1648)
                     /locus_tag="OHN27_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein CapI"
                     /protein_id="HCQ2288804.1"
                     /translation="PGDVLDTSADTQPLYDLVGFKPQTSVKEGVKNFVEWYKDYYQI"
BASE COUNT          412 a          419 c          401 g          416 t
ORIGIN      
        1 caactaattc attccatgac tacttgtcgt tctccagtta actgattaaa gtgtaatctc
       61 atgaaaatta ctatttccgg tacaggttat gttggtttat cgaacggtgt cctgattgcg
      121 caaaaccacg aagtggttgc cctggatatc gtacaggcca aagtggatat gcttaaccag
      181 aagatctctc cgattgtgga taaagagatt caggaatatc tggcagaaaa accgttaaat
      241 ttccgtgcca ccacggacaa gcacgacgcc tatcgtaatg ccgactacgt gatcattgcg
      301 acgccgaccg attacgatcc caaaaccaac tacttcaaca cctctacggt ggaagcggtt
      361 attcgcgatg tcacagagat caacccgaac gcggtgatga tcattaaatc gaccatcccg
      421 gtggggttca cccgcgacat caaagaacgt ttagggattg ataatgttat tttctctcct
      481 gagttcctgc gtgaaggccg tgcgctttac gacaacctgc acccgtcgcg cattgtcatt
      541 ggtgagcgct ctgcgcgtgc cgaacgtttc gcagacctgc tgaaggaagg cgcgattaag
      601 caggatatcc cgaccctgtt taccgattcc accgaagcgg aagcgatcaa actgttcgct
      661 aacacctatc tggcgctgcg tgttgcctat ttcaacgaac tcgacagcta tgctgaaagc
      721 caggggttga acagcaagca gattatcgaa ggggtatgcc tggatccgcg tatcggcaac
      781 cactataaca acccgtcctt tggctacggc ggctactgcc tgccaaaaga taccaagcag
      841 ctgctggcga actacgaatc ggttccgaac aatatcatcg cggctatcgt ggatgctaac
      901 cgtacccgta aagactttat cgcggattct attctcgccc gtaagccgaa agtggtgggt
      961 gtgtatcgcc tgatcatgaa gagtggttcg gacaacttcc gtgcctcttc tattcagggc
     1021 attatgaagc gcatcaaggc gaaaggtatt ccggttatta tctatgaacc ggtgatgcag
     1081 gaagatgagt tctttaactc ccgcgtcgtg cgcgatctgg atgccttcaa acaagaagcg
     1141 gatgtgatca tctctaaccg tatggcggaa gagctggcgg atgtggcgga caaggtctac
     1201 acccgcgatc tgtttggtaa cgattaattc tgcctgtctg acactggcgg ttccctgccg
     1261 ccagtgttct tctttctccc ccgttatatc cctcactacg cgtatttctc gcacattcag
     1321 attaacctca ataaattgtg atggacatta cgatcctgct caaaagccgt tgcgggttga
     1381 aaacaggtcc gataagttaa acttaccttg tgaagagcta ccgtcactgt gatgtgaaat
     1441 gaatgatcaa tgaagggaat acgggaggtt tatccattgc cgcaatcagc tgatcgcggc
     1501 agggcagaga caggttagat ctgatagtaa tccttatacc actcgacgaa gtttttcacg
     1561 ccttccttca ctgacgtctg tggcttgaaa cccaccaggt catacagcgg ctgggtgtct
     1621 gcgctcgtgt ccagcacatc gcccggct
//
LOCUS       DAJOSY010000265         1605 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJOSY010000265 DAJOSY010000000
VERSION     DAJOSY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.268"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..675
                     /locus_tag="OHN27_004502"
     CDS             <1..675
                     /locus_tag="OHN27_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2288805.1"
                     /translation="INIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHIN
                     GQEVPEIILLNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEG
                     AYEVLGVFDKVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRR
                     REDKQNDLWTTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQ
                     FRKWKS"
     gene            complement(972..1574)
                     /locus_tag="OHN27_004503"
     CDS             complement(972..1574)
                     /locus_tag="OHN27_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCQ2288806.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKSERQNQ
                     RRLAYASEVYRIYTGIKSSKGIRLPVTKKSLPEINSVQNN"
BASE COUNT          427 a          340 c          377 g          461 t
ORIGIN      
        1 atcaatatca tcaataagtt acgtgatgaa ggtttccagc cgttctttgc ctgtcagagt
       61 cgggttcgtg atttgggacg tcgcgaatac agtaaacata tgttacgtct tcgcagggaa
      121 gggcatatta acggacagga agttcctgaa attatcctgc ttaattcaca tgatggttca
      181 tccagttatc agatgatccc cggaattttt cgttttgtct gcacaaatgg cctggtgtgc
      241 ggaaataatt ttggtgaaat ccgcgttcca cataaaggtg atattgtcgg gcaggttatc
      301 gagggagcgt atgaagtgct cggtgtcttt gataaggtca ctgataatat ggaggcgatg
      361 aaagaaattc atcttaacag tgacgagcaa catttatttg gcagagctgc actgatggtc
      421 aggtatgaag atgaaaataa aacgccagtg acacctgaac agataattac tccccgtcgt
      481 cgggaagaca aacagaacga tctctggaca acctggcagc gggttcagga gaatatgata
      541 aaaggtggat tatcggggcg aagtgcctcc gggaaaaata ccaggacaag agccattaca
      601 ggtattgatg gtgatataag aatcaacaag gcgttatggg tgattgccga acagttcaga
      661 aagtggaagt catgataata taagtaaagc cccgaaaaaa ttttcggggc tttgcttata
      721 ttatcatttt tcggcattgc tgtgtcgtct gatatacctc aatttcatcg accggctttc
      781 cggttgtctg taaatattgt gcccgcgaaa aatggtctgg caggaggctg cgcagttacc
      841 gggtatatat aagatgggtt cccgcttcgc ggggcttcgg ccagtcagta ttgtcactca
      901 tatgatattt ttgtgtgtgg ccttccatgc cgcttttgcg gcataaccgg tatctgacaa
      961 tgtctgtaag attaattgtt ctgcacgctg ttaatttccg gaagtgattt cttcgtgacc
     1021 ggaagccgga tacctttact gctttttatc ccggtataaa tccggtaaac ctctgatgcg
     1081 taggcaagac gtctctggtt ctggcgttcg gatttcctga atcctgcatt gtatgcacca
     1141 acggcctccc aggtgacgcc ccatttttta aatgctattg ccagataata agcaccggta
     1201 taaatgttca tgcagggatc tgttgtcaga tgttctggct taattccgta gcgggccagt
     1261 tcgttaaaat gctgggaatc tacctgcatc agtccgctgc catatcccgt taccggatta
     1321 ataccgatgg cattaacccg gtaacgggat tctttccatg atattgctct cagtaaatca
     1381 ggatctattt tgtaatcccg gcctgcaaga tcaaagcaat cagtggcatg gcagatctca
     1441 tttataaaca tcaggcagat ggctaacatc cattttttca tttttccacc tctggtgact
     1501 ttatccgtaa ataatttaac ccactccaca aaaaggctca acaggttggt ggttctcacc
     1561 accaaaagca ccacacccca cgcaaaaaca agtttttgct gattt
//
LOCUS       DAJOSY010000266         1575 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJOSY010000266 DAJOSY010000000
VERSION     DAJOSY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.269"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            233..1468
                     /locus_tag="OHN27_004504"
     CDS             233..1468
                     /locus_tag="OHN27_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEc58 family transposase"
                     /protein_id="HCQ2288807.1"
                     /translation="MPRKKKTPVVTPAIDLPKEFLEKLIPGPMDAAGVEAVFQQLKKA
                     VIERALGAELGLHLADAEGGSGNHRNGRSGKTVLTDEGPLRIDVPRDRTGTFEPQLIP
                     KHERRFAGFDDRIVSMYARGMTVREIQGHLAEMYSVEVSPEFISKVTDEVMAEVTAWQ
                     ARPLEAMYPVVFFDALRVKIREDGVVRNKAVYLALGVLADGTRDILGLWIENTEGAKF
                     WMKVFNDLKTRGVNDILIGVADGLKGFPEALEAVFPATTLQTCIVHLIRNSLDYAGWK
                     DRKALAAAIKPIYTAPTAEAAEAELDAFEAGDWGERFPTVVAAWRRAWTRVVPFFAFP
                     PDVRRVIYTTNAIESVHARLRKIIKTRGHFPSDEAATKLIWLALRNITAEWSRASQHW
                     KAAMNQFAIIYEDRFTLAR"
BASE COUNT          299 a          499 c          537 g          240 t
ORIGIN      
        1 agtccgatcc gatgcggcca cgaccccgca gggctgcgct cggccctgtg ccgggtttcg
       61 gtgagaaatg gctacgcact gagccgctgc gtggccccgc gatgtcgaaa acccacgcat
      121 gaacccccga tctgtgcccg atctgtgccc aaagcggcgc ttgcgcggcc gcttccgagg
      181 gtgtcagaaa ttttgtgtga gaggcatacc atgatgctgc caaggagcat gtatgccacg
      241 caagaagaag acgccggtgg tcacgccggc cattgatctg ccgaaggaat ttctcgagaa
      301 gctgatcccg ggtccgatgg acgcggccgg ggtcgaagcc gtgttccagc agctgaagaa
      361 ggccgtgatc gagcgggcgc tgggcgcgga gctcggcctg cacctggccg acgccgaggg
      421 cgggagcggc aaccaccgca acggccgcag cggcaagacg gtgctgaccg acgagggtcc
      481 gctgcgcatc gacgtgccgc gcgaccggac gggtacgttc gagccgcagc tgatccccaa
      541 gcacgagcgt cgcttcgccg ggttcgacga tcggatcgtc tcgatgtacg cccgcggcat
      601 gacggtgcgt gagatccagg ggcacctggc cgagatgtac agcgtcgagg tgtcgccgga
      661 gttcatcagc aaggtcaccg acgaggtgat ggccgaggtc accgcatggc aggcgcggcc
      721 gctggaagcg atgtatccgg tggtgttctt cgacgcgctg cgggtgaaga tccgcgagga
      781 cggcgtggtg cgcaacaagg cggtgtatct ggcgctgggc gtgctggccg acggcacccg
      841 cgacatcctc gggctgtgga tcgagaacac cgagggcgcg aagttctgga tgaaggtgtt
      901 caacgacctc aagacgcgcg gcgtcaacga catcctgatc ggcgtggctg acggcctgaa
      961 gggcttcccg gaggcgctgg aagcggtgtt cccggcgacc acgctgcaga cctgcatcgt
     1021 gcacctgatc cgcaacagcc tggactatgc gggctggaag gaccgcaagg cgctggcggc
     1081 ggcgatcaag ccgatctaca cggccccgac cgccgaggcc gcagaggccg agctggatgc
     1141 gttcgaggcc ggcgactggg gcgagcgctt cccgacggtg gtcgccgcct ggcgccgtgc
     1201 ctggacgcgc gtggtgccgt tcttcgcgtt cccgccggac gtgcgccggg tgatctacac
     1261 gaccaacgca atcgagagcg tgcatgcgcg cctgcgcaag atcatcaaga cccgcggcca
     1321 cttccccagc gacgaggccg ccaccaagct gatctggctg gcgctgcgga acatcaccgc
     1381 cgagtggagc cgcgcgtctc agcactggaa ggcggcgatg aaccagttcg cgataatcta
     1441 cgaagaccga ttcacgctgg ctcgctgaaa gagacttccg gactaaaccg cctcacacac
     1501 aaaaaatcgg acacgccccc gcttcctacc tgccagactc gcaaaaaagg gcggttgaac
     1561 ttcctatacc cacaa
//
LOCUS       DAJOSY010000267         1573 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJOSY010000267 DAJOSY010000000
VERSION     DAJOSY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.270"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(945..1421)
                     /locus_tag="OHN27_004505"
     CDS             complement(945..1421)
                     /locus_tag="OHN27_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2288808.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          347 a          420 c          396 g          410 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgcttac tcctctgcaa gcctcgccag
      181 ccgtgatttt tgccagattt cgtggttttc acgttcaaca gctcctgcac gttttttgcc
      241 gtccgttcac cgttaacccc cgcagaaatc gggacaggat gtgcaaatgt aataccgtca
      301 cccgcgacga tattccgttt gccatctggt cagggcttcg ccccgacacc ccgtatcagt
      361 gcgtacgtgc tcaatgtggt ttccggttct cttccgcttc ctcgcccact cgctcactaa
      421 cgttcggtcg ctggggctgc ggcgagcggt gccacctctc gcatgttgtc ggtgattccg
      481 ctgttgttct ttcggggatg agtcgtcaga tagcccttca aacgcgctca gaagctctca
      541 gacgcacgag gcggggttta aatgcccgtc agggcatgga aggcgcgaaa aacgcttaca
      601 gagcgtttca ggaggtcagg cgcgccgata cccctagcca ggggctttca ggtcgccagg
      661 aaccgcgcca gccctctgca aagcccattt gcacggtaca ggcagtcact cggttggtag
      721 gtggtcgtag accgtcggtg ttacagcgat gggggtctgg ggttctcccc agacggtttc
      781 cggttgcgct caaggcgctg ccggaaccgt ggcggctcgg tcgttctgga gatcggggag
      841 caaaaacacg caagggattt tgaggcggca aaagagcaat gcaggataga caatcatggt
      901 cagaacgtta acacgtagcc cctgattgtc cgcctgccgc tggctcaatt tgaaaacatg
      961 gccatgattc ggcgctgaat atcaccgtct ttgttgacca gtgttttagt gacaaatgct
     1021 gcatggtttt tcgtagtgtt gtatcccttc cagacttcat gtggatcgag ggcatacgca
     1081 caaaccttac cagcaacggg gattctaact aatgcatttt ttgactctaa gtaattgagc
     1141 caacgaataa ttgtgcttct gctaactcca agtttttcag ctaggaattg ctgatccgca
     1201 acgacagcgc cacacgtagg atcgatatgc tccgctaaaa atgagtacag cttcagtgct
     1261 gatatgcctt ggctatcctt cagcagctct cgaacgcgtt cccaaccttt tggggatacc
     1321 tgtgtaaacc ttgaattctt ctttgcttct tcgagatcct cggtgttttt ctttgcctca
     1381 tctgaaagtt gtttcagtcg tttttctgaa tcaataaaca tgattttttc cctctaaaaa
     1441 tcccatgcac taacgttggt tcaaatctaa gccatcatga cacatctgtg caacaccttg
     1501 ttgcgtgttg tcgcatcctg gatgcgacaa cgttgtgtca ccctggatgc gacaggtaaa
     1561 caaaaaacac act
//
LOCUS       DAJOSY010000268         1567 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJOSY010000268 DAJOSY010000000
VERSION     DAJOSY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.271"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            8..868
                     /locus_tag="OHN27_004506"
     CDS             8..868
                     /locus_tag="OHN27_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCQ2288809.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
     gene            complement(900..>1567)
                     /locus_tag="OHN27_004507"
     CDS             complement(900..>1567)
                     /locus_tag="OHN27_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2288810.1"
                     /translation="FLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRAL
                     MLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQG
                     SLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQR
                     ADR"
BASE COUNT          280 a          475 c          480 g          332 t
ORIGIN      
        1 cgatttattg ttcacagcga tagcacctgc catttggggc agcacctaca ttgtcaccac
       61 ccaatacctg ccgaacttct caccgatgac ggtcgcgatg ctgcgggcgt tgccggcggg
      121 tttattgctc gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt
      181 catcctcggc gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg
      241 cctgccgggc ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat
      301 ctctgccgct ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg
      361 aactgcgggc gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt
      421 cgcagcgggc ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg
      481 gcaacctccc gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct
      541 gctcgttcca gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt
      601 tctcggcctg gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg
      661 ggggatctcg cgactcgaac ctacagttgt ttccttactg ggctttctca gcccggggac
      721 cgccgtgttg ctaggatggt tgttcttgga tcagacgctg agtgcgcttc aaatcatcgg
      781 cgtcctgctc gtgatcggga gtatctggct gggccaacgt tccaaccgca ctcctagggc
      841 gcgtatagct tgccggaagt cgccttgacc cgcatggcat aggcctatcg tttccacgat
      901 cagcgatcgg ctcgttgccc tgcgccgctc caaagcccgc gacgcagcgc cggcaggcag
      961 agcaagtaga gggcagcgcc tgcaatccat gcccacccgt tccacgttgt tatagaagcc
     1021 gcatagatcg ccgtgaagag gaggggtccg acgatcgagg tcaggctggt gagcgccgcc
     1081 agtgagcctt gcagctgccc ctgacgttcc tcatccacct gcctggacaa cattgcttgc
     1141 agcgccggca ttccgatgcc acccgaagca agcaggacca tgatcgggaa cgccatccat
     1201 ccccgtgtcg cgaaggcaag caggatgtag cctgtgccgt cggcaatcat tccgagcatg
     1261 agtgcccgcc tttcgccgag ccgggcggct acagggccgg tgatcattgc ctgggcgagt
     1321 gaatgcagaa tgccaaatgc ggcaagcgaa atgccgatcg tggtcgcgtc ccagtgaaag
     1381 cgatcctcgc cgaaaatgac ccaaagcgcg gccggcacct gtccgacaag ttgcatgatg
     1441 aagaagaccg ccatcagggc ggcgacgacg gtcatgcccc gggcccaccg gaacgaagcg
     1501 agcgggttga gagcctcccg gcgtaacggc cggcgttcgc ctttgtgcga ctccggcaaa
     1561 aggaaac
//
LOCUS       DAJOSY010000269         1554 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJOSY010000269 DAJOSY010000000
VERSION     DAJOSY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.272"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            620..955
                     /locus_tag="OHN27_004508"
     CDS             620..955
                     /locus_tag="OHN27_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2288811.1"
                     /translation="MPYMAKRYQTENGEIPYTDWMKKLRRKDQAAALKVDSRIARAMS
                     GNFGDHKFERDGVWELRIDYGPGYRVYYSIEDGEIILLLIGGNKKTQTADLDKAVSYL
                     QDFKKRSKK"
     gene            952..1302
                     /locus_tag="OHN27_004509"
     CDS             952..1302
                     /locus_tag="OHN27_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCQ2288812.1"
                     /translation="MTATKNVKSQKNGYPASVDHNAAMIEELRADPDYANAYLANALE
                     EINEPGGLGGFLVALRQVIEARGGISEAARKSGLARQSIYRALSPNGNPTITTLAQLT
                     SVAGLQFTLSKSSH"
BASE COUNT          419 a          375 c          395 g          365 t
ORIGIN      
        1 tgtcctgtgg acagacagat atgcacctcc caccgcaagc ggcgggcccc aaccggagcc
       61 attttagtta caacactcaa atacaaccac caggaaaacc ccggtccagc gcagaaccga
      121 aaccacaaag cccctccctc ataactgaaa agcgaggccg cccccgccca aaaggcggga
      181 ataacatcgc ttttaattat gaatgttgta actacattat catcgctgcc agtcttctgg
      241 ctggatgttc tcagtacacg ctcgtaagcg gccctgacgg cccgctaacg cggagatacg
      301 ccccgacttc gggtaaaccc tcgtcgggac cactccgacc gcgcacagac actctgtcat
      361 ggctgaaagc gggtatggct tagcagggcc ggggatgggt aaggtgaaat ctatcaatca
      421 gtaccggctt acgccgggct tcggcggttt tactcctgtg ccatatgaaa caacggagta
      481 acgccttcca tgccgctggc gcggcatatg ccggcagtac agctatctgt gtttgatagt
      541 atttaaagcg acgttttttc ttgaactgct gtcacttata agagacaata tgcaaggtta
      601 agggaatgag ggtggcaaga tgccttacat ggcgaaacga tatcagacag aaaacggtga
      661 gatcccatac accgactgga tgaaaaaatt aagacgcaaa gaccaggccg cagcgttaaa
      721 ggtggattcc agaattgccc gcgctatgag cggaaatttc ggagatcata aatttgaaag
      781 ggacggggtc tgggagttac ggattgatta cggtcctggt tacagggttt attattcaat
      841 cgaagatggc gaaataattc ttctgctcat tggcgggaat aaaaaaactc agacagccga
      901 tttagataaa gccgttagtt atctgcaaga ttttaaaaag aggtcaaaaa aatgacagcc
      961 accaaaaatg tgaagtcgca gaaaaatggt tatccggcat ccgttgatca caatgccgcc
     1021 atgattgaag aattgcgcgc agatcctgat tatgcaaacg cttatcttgc taatgccctg
     1081 gaagaaataa acgagcctgg tggtttaggc ggttttcttg ttgccctgcg tcaggtcatc
     1141 gaggcgcgag gggggatttc cgaagctgcc aggaaatctg gccttgcccg tcagagtatt
     1201 taccgcgcat tatccccgaa cgggaatccg accatcacaa cacttgcaca acttacatca
     1261 gtagccggat tacagttcac tctcagtaaa tccagccact aatctctccg gttccccctg
     1321 agcgggggaa tttcactggc acttattgcg gggaacaaaa ccatcggacg ggggaaacat
     1381 ctgtatttct gtgtgtcccg gtgtaatccg gtggatttgt gtgtgtctgg aagcttaatg
     1441 ctaatgtatg cgtacattag cattaagcct gttttcccgt cgtgtcgggg tcagtctggg
     1501 aaacgtacaa tattgtgcat ttcttgctgt aggggaacga ggtaacgttt acac
//
LOCUS       DAJOSY010000270         1545 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJOSY010000270 DAJOSY010000000
VERSION     DAJOSY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.273"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            150..1526
                     /gene="ygeH"
                     /locus_tag="OHN27_004510"
     CDS             150..1526
                     /gene="ygeH"
                     /locus_tag="OHN27_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HCQ2288813.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYR
                     AMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
BASE COUNT          559 a          265 c          262 g          459 t
ORIGIN      
        1 actgaattcc taagataaat tagtgaacac aaccatagat atgtcgaatg taatttcctg
       61 tagcattaat gacacataaa tatgaatagc cataatttct attgcttaag caggatgcaa
      121 gaaaccaatt ttttcataga ggttaactaa tggacttaga aaataaattc tcatatcatt
      181 ttcttgaggg attaacgctc acggaagatg gaattcttac tcaaggaaat gagcaagttt
      241 atattccaca gaaagagtta ggtgtattaa tagtattact tgaatccgct ggtcatgttg
      301 tactgaaaga tatgatcatc gaatcagtat ggaaaaatat tattgttagt gacgagtccc
      361 tgacaagatg tatctattct ttgcgctgca tttttgaaaa aattggctat gatcgttgca
      421 tagaaacaat ctaccggaaa ggttatcgtt tcagcgggca ggttttcaaa actaaaatta
      481 atgaagataa tacttcagac tattccatag ctatattccc tttcactact tcattgaata
      541 cactggatcc attaatactt aatcaggaat tagtgcaaat catttcaaat aaaaaaatcg
      601 atggtctcta tacctatccg atggctgcga caaatttttg taatgatcac atatctcaaa
      661 attcattctt gagcagattc aagccagatt atttcgttac aggaagaata aaccagaata
      721 atgcagtgaa cactttatac attgagttga tcgacgctaa aaaccttttc ctcatcgcca
      781 gtaatcatct ccctgttgat gaactacata atacatcaca atttattata gataatatcc
      841 ttcaaacggt acataaacca gaacgatctg taagattagc taagcaggac caaggatata
      901 agaatcatta tttatcagat gaaatgttag ccggaaagaa agaactttac gacttcaccc
      961 ctgaaagcat ttacagggcc atgactatat ttgatagatt acaaaataaa agtgatatac
     1021 agacgctaaa aacagaatgt tattgccttc tagcggaatg ccatatgtct ttggcacttc
     1081 atggaaaaag tgaacttgaa cttgctgctc aaaaagcatt agagctttta gattatgtat
     1141 cagacataac cactgtcgat ggaaaaattt tagctattat gggactgata actggtctgt
     1201 ctggacaagc aaaagtatct catatcttat ttgaacaggc taagatacac tcaactgata
     1261 tagcctctct ctactactat agggcactag tccactttca taatgaaaaa attgaagagg
     1321 caaggatttg tatagacaaa tcactacaac tcgaacccag aagacgaaaa gcagttgtga
     1381 taaaagaatg tgtagatatg tatgtgccta acccgctcaa aaacaacatt aaactctact
     1441 ataaagaaac tgagagtgaa agccatcgag ttataattga caacattttg aaattaaagc
     1501 agctgacgag aatttgtatg cgataaattt ttattgacgc aattt
//
LOCUS       DAJOSY010000271         1525 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJOSY010000271 DAJOSY010000000
VERSION     DAJOSY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.274"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            3..635
                     /locus_tag="OHN27_004511"
     CDS             3..635
                     /locus_tag="OHN27_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCQ2288814.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSTAQIFILGEFAGGMMGSELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
     gene            complement(654..1155)
                     /locus_tag="OHN27_004512"
                     /pseudo
     CDS             complement(654..1155)
                     /locus_tag="OHN27_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(1238..1456)
                     /locus_tag="OHN27_004513"
     CDS             complement(1238..1456)
                     /locus_tag="OHN27_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288815.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMASFQNDMYSEDNPISPYYKIEW"
BASE COUNT          475 a          237 c          286 g          527 t
ORIGIN      
        1 atatgggaaa aattaaaatt gtagtttcag atcagcagcc gtttatgatt gatgggataa
       61 ttggatttct cggacattat cctgatttat atgaggttgt tgggggctat aaagatctga
      121 agaaagctat agcggagtgt aacaaatcta cagcccaaat atttatttta ggagaatttg
      181 caggtggaat gatggggtcc gaactcgtaa aatgggttaa atcgcataag atagatgctc
      241 atattattac atttgtagca aagatgccgt atattgattc aataaaattg cttgaagcag
      301 gtgcgaaagg atgcgtatgg aaaaccagtc acccggcgaa actaaatcgt gctattgatt
      361 cgattagtaa tggttacact tattttgata gtgtacatat ggattgtgaa aaaatatctt
      421 ccaggtattc ctctgataat caacttacaa atcgtgagtc agaaatatta caactcatag
      481 ctgatggtaa aacaaacaaa gaaatcgcta atttcttgca gttaagcagg aaaacagtcg
      541 aaactcatag actcaatatc atgaagaaat tagatgttca cagtggtatc gagttgatca
      601 aaacagcttt acgtatgggt gtttgcacta tataaaaaaa agaggcttta ggctcatttt
      661 gctgaagcct tacgattgtc tagttcatta agcctggtat atacaatata tattttgggg
      721 catattgctg tctctttttt tggttatcca ttgcaaaacc cgcattataa gtccccaggc
      781 attgccacgt tactccacaa cgtgaaaaat ggttatataa aatctcggtt cctattttaa
      841 tgttcagaca agggttatct aacaaatctc gttcatcttt tattattcca cgttttttta
      901 acaggggaat atgcatctgg tttatctgca ttaacccata atctaaactc tttatattgt
      961 tattcttatc tctgttcttg cctatgctat ccttttttag attgctttca acaagagcga
     1021 tagctttaat taaatttggt tcgattctga acattgttcc tgcttcatta gagcaagagt
     1081 tgctggcgaa agactgtttg taataaatat acaaaacgtg aagatggtta agaatttaac
     1141 ttttggtgag tgcatatttt ttcatgtgtg aggattgaat gaaaggaacg agatactatt
     1201 ataaaagtat ctcgttatga tttatttggt caatcgtcta ccattcaatt ttatagtaag
     1261 gtgagatagg attatcttct gaatacatat cattttgaaa agacgccatc catgttgaaa
     1321 ctataaatgc ttgtatttga tctgtatcag tagcactggt gttgatgtgt ttatctaatg
     1381 atacgacttc atcacccgtt gcattcgcta tatttgaact ttgagataaa ttattgatac
     1441 cgattggatt agtcataaag ctcccttttt taattgtcca ggccaatggt ctttacatca
     1501 tacttttaac tctagagcat tttaa
//
LOCUS       DAJOSY010000272         1466 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJOSY010000272 DAJOSY010000000
VERSION     DAJOSY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.275"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            11..226
                     /locus_tag="OHN27_004514"
     CDS             11..226
                     /locus_tag="OHN27_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HCQ2288816.1"
                     /translation="MAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEH
                     GDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            223..504
                     /locus_tag="OHN27_004515"
     CDS             223..504
                     /locus_tag="OHN27_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2288817.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            544..1392
                     /locus_tag="OHN27_004516"
     CDS             544..1392
                     /locus_tag="OHN27_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCQ2288818.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
BASE COUNT          349 a          366 c          442 g          309 t
ORIGIN      
        1 ggatgctttt atggccgctg caaaaagcat ggaccgtaat ggctctcagt taatccggga
       61 ttttatgcgc cagaccgttg aacggcagca taatacctgg ttccgtgacc aggttgcggc
      121 aggacgtcag caactcgagc acggcgatgt gcttccccat gacatggtcg aatcctctgc
      181 tgcagcatgg cgggatgaaa tgagcaggaa ggttgccggt aaatgatgga gatattctgg
      241 accatgctgg ccagccagga caggaagcgc attcgtgagt acattgcgga gcagaacctg
      301 atggctgcca ttgaactgga cgaacggatt ggttattcag ccagcagcct ggccgggcag
      361 ccgtataaag gccgcaacgg tcgtgtggaa ggtacccggg aactggttat tcacccgcat
      421 tttgttctgg tttatgaggt cgacagccag tggggaaaag tgtatatcct gcgcgtgttg
      481 catactgcac agaagtggcc atagaggggc tgtccggata taaggaaagg agatgcaata
      541 accatgaata atgatgagct ggccaccaga cgtgcgcagg cgattgccga agacaggtgt
      601 ttctcgaaag gacgtttgcg cgatgaattc agaatgaaac cagcccccgg tgctgagccg
      661 gttaagtggt ataaaaacac ttatggtggc aggttcgctg tataccgaat tgcagactgt
      721 gttccgatgc gtgagaaacg ccctctgacg agtaaacaac agctggctgg ccagcgactt
      781 tccgttttat ccagacttaa cagcaccagt ggccggatgg cacggcaggc atatgactgg
      841 ctgagtctgg caccgctctt tctggatact gaaacgacag gactggataa taccgcggaa
      901 gccctggaaa tcggcctgac cgatgcggcc ggtcaggtgg tatttgaaac caggctgaag
      961 cccacggtcg ccatcggggc gcaggccgcc gctgtgcatg gtatcagcga gcaggctctc
     1021 tgcggcgcac catcatggac agatgtggcg cggcaactgc gtcatgcaat cggggaccga
     1081 ccagtaatta tctttaatgc ccggttcgac atccgcattc tgaaacagac tgctgccgca
     1141 catagcgatc cggctgactg gctggaagaa ctgacggtat attgtgcgat ggagctggct
     1201 gcaggatatt atggggccac caaccgctat ggcacgattt cactggcctg tgctgccagc
     1261 caggccggac tgacctggga agggcaggca cactcagcga tcgccgacgc acggatgacg
     1321 gcaggggtgg taaacgctat tgctgcatat catctggaac tgctgcagga acaggcacgg
     1381 ctgaaaatct gaccgcctgg cctgtatacc gcaatcattt acgttatcca gacgtaaatc
     1441 cgccgcttgt tgctgtcagc atgtta
//
LOCUS       DAJOSY010000273         1459 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJOSY010000273 DAJOSY010000000
VERSION     DAJOSY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.276"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            77..271
                     /locus_tag="OHN27_004517"
     CDS             77..271
                     /locus_tag="OHN27_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCQ2288819.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEELQNSIQTRFEAENRTGI"
     gene            complement(487..1347)
                     /locus_tag="OHN27_004518"
     CDS             complement(487..1347)
                     /locus_tag="OHN27_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HCQ2288820.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          430 a          337 c          338 g          354 t
ORIGIN      
        1 cggtaaaaca gtcgaaaagt ggtacacgat ataatcacac aacgcagatg acagaaagaa
       61 cggagaaaac agaaagatga acaagcagca gcaaaccgta ctcaacatgg caggattcat
      121 aaaaagccag agcctgacgc tgctcgaaaa actggacgca cttgatgctg acgaacaggc
      181 cgccatgtgt gagaagctgc acgaactcgc ggaagaactt cagaacagca tacagacacg
      241 ctttgaagcg gaaaatcgca ccgggatata acgcatccgg acaggatgca gaacggcata
      301 gaggagcgta aggagaaaat gtcacataag gcgctcttcg gggtctgacg ctcagtggaa
      361 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      421 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
      481 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
      541 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
      601 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
      661 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
      721 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
      781 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
      841 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      901 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg cagcagtgtt
      961 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1021 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1081 gagttgctct tgcccggcgt caacacggga taataccgca ccacatagca gaactttaaa
     1141 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1201 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1261 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1321 ggcgacacga aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1381 ccagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     1441 aggggttccg cgcacattt
//
LOCUS       DAJOSY010000274         1448 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJOSY010000274 DAJOSY010000000
VERSION     DAJOSY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.277"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..867)
                     /locus_tag="OHN27_004519"
     CDS             complement(<1..867)
                     /locus_tag="OHN27_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCQ2288821.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNTNVEVRIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQNYFNEAYQ"
     gene            complement(893..>1448)
                     /gene="rfaY"
                     /locus_tag="OHN27_004520"
     CDS             complement(893..>1448)
                     /gene="rfaY"
                     /locus_tag="OHN27_004520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCQ2288822.1"
                     /translation="VSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETD
                     RVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASM
                     EKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIK
                     DYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          455 a          302 c          248 g          443 t
ORIGIN      
        1 ctgataagcc tcattaaaat agttctggca gggataattc gtccagtcgt tccagggctt
       61 cgttacgcca acataatgaa tcagcttagt atcatcggta atggtctttt tataatcttc
      121 atcgctttta ttttttaact cataatcgat gccataaaag caatcataat cgccgctaat
      181 aaaaatacaa tgaccaaaaa acagaatatt taaaatatcc tgatcaaggc atttatactt
      241 cgtgtgctgc gggtcacttg ccagcatagc aatggcttta ttcataaaat catttttcgc
      301 ccatgcttca agctgcagat atactacacc ggcgttgaaa taattccctg gaagtccctc
      361 tatagccaga cgcttcgctg gtttttcctg cataaaaggc acatctttaa taacagctgc
      421 aaacttatcc ccatcaaaat taatatcaag caatccagct aatgagcctt tacagatgac
      481 atcagcatcg atatataaca gattttcaat agtttcactc agatactgga acgcaactaa
      541 acggaagaat gttgcataag accactgttt caggctgggg aagatatcaa taaaggcatt
      601 ggagacttta taaattctta cttcaacgtt agtatttttg ctggtaaaag cattaacacg
      661 ttgtagataa tcttcatcaa tgtagtcggt aaagagatga aacttaaccg caaggtccgg
      721 gttatgcata acaactgatt gcatagatac tgccgcacca aataaaaagt tacggtcaat
      781 accgtaagcg atatttaaca cacgatcggt gttagcaatt tccccagaga cagcgacttc
      841 agtaatactc tcagctaata aatccatgtt ggttccgttt tgactgtgtg gtttacgctt
      901 tgccttttaa ttttttaata aatttgcgta gtttggttct gtagattact gaatagtagc
      961 cataatcttt tatttcgttg gcaataccca ggtgacgttc catggccaga cgatcacgag
     1021 ctttacgctc tgccgtacag cttttaccgg ataggtcaat tatcctgaca ccatctttac
     1081 taacgataaa attaccccgg tgtgggtcgc ccgatagcat atttaaggca tgcaattttt
     1141 ccattgaagc cttaatttct gctttaacat tttctggaat aatcggcata tcattgagct
     1201 ctacgccctc gacatactcg ataagcataa taaaaacact ggcatagttg aaaattttac
     1261 gctcagccag aaaataaaaa tcattaggaa aagtaagacc tgcacttctg acacgatcgg
     1321 tttcaacgat caaattctga tagtagtcac cttttacaaa agattttaaa aaacgttcat
     1381 tccttttcgt tttcggggca aagactttaa aaacatagcg accatatttc gtatcaatca
     1441 atgatact
//
LOCUS       DAJOSY010000275         1426 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJOSY010000275 DAJOSY010000000
VERSION     DAJOSY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.278"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1087
                     /locus_tag="OHN27_004521"
     CDS             <1..1087
                     /locus_tag="OHN27_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2288823.1"
                     /translation="ATGFLGGAVLDKLLDNCNNINLLLLVRAPTPQAGLERIKENMRK
                     FNVCEERLHALTNDNILPGDLNNPEAFLMDPRLDEVTHVINCAAIASFGNNPFIWNVN
                     VTGTLAFARRMAKVAGLKRFLHVGTAMSCTPHTGSLVKEESASSETGEHLVEYTHSKA
                     TIEYLMRKQCPDLPLLVARPSIIVGHSRLGCLPSTSIFWVFRMGLMLQKFMCSMDDKI
                     DVIPVDYCADALLMLLESSLINGEIVHISAGKESSVTFSAIEEAVARALNCDPVGDRY
                     TKVSYDILAMSRHDFKNIFGPCNERLMLKAIRLYGAFSMLNVCFSNDKLLSIGMPKPP
                     KFTDYIKYCIETTKHLSIQQQMEVDFK"
     gene            1156..>1426
                     /locus_tag="OHN27_004522"
     CDS             1156..>1426
                     /locus_tag="OHN27_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350955.1"
                     /note="induced within macrophages; necessary for the
                     resistance to antimicrobials; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antimicrobial resistance protein Mig-14"
                     /protein_id="HCQ2288824.1"
                     /translation="MSIKNIKRIITAWKPSTFETYKKTFEKYGGSVNMHPDVVSYFMI
                     HHDWKFDFFHYEKDGDIKGSYFLCNGKQIGIMARRSYPLSSDEVLI"
BASE COUNT          443 a          229 c          296 g          458 t
ORIGIN      
        1 tgcaacagga tttcttggtg gcgcggtcct ggataagctg ctggataact gtaataatat
       61 aaatttgcta cttttagtac gagcacctac tccacaagcg ggactggaaa gaattaaaga
      121 aaatatgcgt aaatttaatg tttgtgagga aaggttgcat gcattaacta atgataacat
      181 cttgcctggg gatctaaata atccggaagc ctttctcatg gatcctcgtc ttgatgaagt
      241 cactcatgtt ataaactgtg cggctatagc ttcttttggt aataatcctt ttatatggaa
      301 tgtgaatgtt acaggtacac ttgcttttgc aagaagaatg gcaaaagtgg caggactgaa
      361 acgcttcctt catgttggta ctgctatgtc ttgtacacct catacggggt cgctagttaa
      421 ggaagagtct gcttcatcag aaacaggtga acatttagtg gagtatacgc attcaaaagc
      481 aacaatagaa tatctgatgc gtaagcagtg tcctgattta cctttgttgg ttgcccgacc
      541 atcaattatt gttggccaca gtcgtttagg gtgcttacct tcaaccagta ttttctgggt
      601 attcagaatg gggttaatgt tgcaaaaatt tatgtgctct atggatgata aaatagatgt
      661 tattcctgta gattattgtg ctgatgcatt gctaatgttg cttgaaagct cgttaattaa
      721 tggtgagatt gttcatatat cagcaggtaa agaaagtagt gtgacgttct ctgctattga
      781 agaagctgta gcccgtgctt tgaactgtga tcctgttgga gacagatata ctaaagtcag
      841 ttatgacata ctggcaatga gccgtcatga ttttaaaaat atttttggtc cctgtaacga
      901 acgccttatg ttaaaagcca ttcgtttata tggagcgttc agtatgctca atgtttgttt
      961 cagtaacgac aagctactga gtatcggaat gcctaaaccg ccaaagttta ctgattatat
     1021 taaatactgt atagaaacga caaaacacct ttcaattcaa caacaaatgg aagttgattt
     1081 taaataaaat ttatcataat tagaattatt gttggtgttg aactcataat atataaaaac
     1141 gccgaggata tgactttgtc aattaaaaat ataaagcgca taattacggc ctggaaacca
     1201 tctacattcg aaacgtacaa aaaaactttt gaaaaatacg gtggtagtgt aaacatgcat
     1261 ccggatgttg tttcatactt tatgatacat catgactgga agtttgattt ttttcactat
     1321 gaaaaagatg gtgatatcaa aggctcatat tttctgtgta atggaaaaca aattgggata
     1381 atggcaagaa ggtcttatcc tctctcatct gatgaggtat taattc
//
LOCUS       DAJOSY010000276         1409 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJOSY010000276 DAJOSY010000000
VERSION     DAJOSY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.279"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            58..1404
                     /locus_tag="OHN27_004523"
     CDS             58..1404
                     /locus_tag="OHN27_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288825.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRLRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
BASE COUNT          376 a          304 c          354 g          375 t
ORIGIN      
        1 gtgttctttg tatattttac ttactgttta agcatgtaca taaggagtgc tttcattatg
       61 ccaatgatac ttgtaaagaa gaatccccgg ggcaaagtga ttcgggagct tagcagtgag
      121 gaagagactg cagtaaagac ggtctgtgga ctgaaaaagc cagcaaccat ggcactacat
      181 aacctggcca atgatattct ccgggaaatg cgtgagtatg atgcatggtt acagtgtgac
      241 tgtattccgg gagattcgcc ggccatgaat tttgctgctc ttaaaaacaa tacgggtact
      301 ctttatctgt ccagttttaa tcatgagcat gcacctgaat gccccatgta tcgtcagtta
      361 tccggaaatg aagaggaaac atcttccgga gcaagccggc atcccgttag cacccgtata
      421 aattacagaa actttttacc acctgatgac agtaattctg ttatcagact gcaggcccgc
      481 tctgcttacc ataatggtga gagctcctcc gttcgcaaga aacgtctgcg ccttgcccgt
      541 ttactcctgt ctcttattga cgatgctggg ctgaataaac tggattccct ggctaatccc
      601 cgtatccgga ctaattatcg tgagtgtctt gatgccatac ggcaggttac actgcagcag
      661 gagtatattc ggggaagatc cttatctgaa attatccatt tccggcctgg aatgtctgaa
      721 aggtctcagg agcggttaat ggaaacgctg gagaattcag aacgacactg gcctgccaga
      781 agaaagcata tgttctttca gatttttatg gcgcagcaca tctgtcgtga tgctgtggaa
      841 attcactggg cgaacggtaa tattcaggtc atcagacctg tgcgggggat cagtattaat
      901 ggtgaagcgc agggcggaat acgtccccct tactgggtta ttcttgcttt ttgccggagt
      961 gccgatggca gaatcatctg cagtgaaggg tatgctcatg ctctttatca actgacatgt
     1021 ccggtgcctg tggacagcaa actggaacga aacacgctca ctgctcttct gaatgtggcc
     1081 agctggctta aaagaaagcc aggtacgccg gaattaagtc tggaaaggcc cctgtttgat
     1141 acagaagttt atgttaatgg tgaaaagaaa tatgtactgc cggatttcat tgttacagca
     1201 agggctcctg acggaaagac ggccagagtg gtcatcgaaa cgatgggata tgaagacagt
     1261 gattactgcg cgagaaaatc caggcagcat accggcatga agcagattgg tgttctgcat
     1321 accgatccac cgaaatggct ggataacgat catccccctt ttgagaaaca tatgtacggt
     1381 gtttttatgc atctcaggta ctgagatat
//
LOCUS       DAJOSY010000277         1408 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJOSY010000277 DAJOSY010000000
VERSION     DAJOSY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.280"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..487)
                     /locus_tag="OHN27_004524"
     CDS             complement(<1..487)
                     /locus_tag="OHN27_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288826.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPGGKCIITGDFQKELAYLQNVILYNVSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNININCPLKDIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     VD"
     gene            complement(509..1048)
                     /locus_tag="OHN27_004525"
     CDS             complement(509..1048)
                     /locus_tag="OHN27_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288827.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYHGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1304..>1408)
                     /locus_tag="OHN27_004526"
     CDS             complement(1304..>1408)
                     /locus_tag="OHN27_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCQ2288828.1"
                     /translation="FKISNTCIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          438 a          248 c          257 g          465 t
ORIGIN      
        1 aatcgacatt acgtaagtcc agagaggata gatcaacgat atctccagtc ccacaactaa
       61 aaattacgtt taacctcctg atttcattgt aaatatcttt taatgggcaa ttaatattga
      121 tattacctgt ggatatttcc tggtttttta acggttcatt aatggtaagt tcacaagtat
      181 tgtccgatcg ttgaataatt tgggcgttat aacctaaaaa atccagacga agagacgaga
      241 cattataaag aattacattt tgtaagtacg ctaattcttt ttgaaaatca ccagtgataa
      301 tgcattttcc accaggttgt aacgtgttaa gtaaagtgac catttcaata gaggtctgtt
      361 ttagtttttc tatacctggt gctagtgctt cgaggttatt tcctgttggg ctttcaggaa
      421 ggtcattcat ttttcgacgg actaaatcac tactaatatc attaccaatt gatgaaacag
      481 ggaacattat tttatccttt ataaataatc aataaaaatc attattaaaa tgatcgggtg
      541 aaaaaaagag aatttgccaa caatctgaag taatgctggt tttattaaag tgcacaaggg
      601 gtgttaagat ggcttctttt cgggtgaatt ttgcacaata cttagaaatc gtctccttaa
      661 tacattccag ctctttttta ttgacggctg agcaagtaca ttgttcacca aaaggtgcaa
      721 aagtgcctac atctaatgtt tgtatgatat aatcatcatg ttctcctgta gaaatatcta
      781 taatgaatgg actcatattt tccggttgga ttcggatcat gacagaagga acatcgccat
      841 gatagaaatt caatgcagtt attgcagata atactctccg cagttcatta cttatactgt
      901 aaattttatt aacgtcattg gtggagacaa tttctgcaaa ctttcttttt accgtccaat
      961 aatcaatatt tctattgtga ttgttaacca ctgcgtgcat tggcgggatt gaagggtgaa
     1021 aaatatgcgc aattgggaag cctttcattg atgcctgcct tgtcatcatg acaaacaata
     1081 atagatgctt attttcctcg cagggttctt tcgcactatc aaaaaacgct catttacccg
     1141 gcgttacagt aaacgaagca acattctgtg gcctacgccg atcaaaagct ctgtgaaaat
     1201 ttcccttgtt gtctttgtct gaccttatcg cggcgatcag caattattcg ttttgtgatc
     1261 ctcaacacaa aatgctattc gtcgcctatg aggggagaag aatttatgga aaacgattgc
     1321 attcacaaaa tgacgtcctg tcagaagaga aaaagttgac gttaatcaat tgttcgagat
     1381 gatcaataca tgtattcgaa attttaaa
//
LOCUS       DAJOSY010000278         1405 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJOSY010000278 DAJOSY010000000
VERSION     DAJOSY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.281"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            138..542
                     /gene="stpA"
                     /locus_tag="OHN27_004527"
     CDS             138..542
                     /gene="stpA"
                     /locus_tag="OHN27_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCQ2288829.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(589..1113)
                     /gene="ygaP"
                     /locus_tag="OHN27_004528"
     CDS             complement(589..1113)
                     /gene="ygaP"
                     /locus_tag="OHN27_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCQ2288830.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1123..>1405)
                     /locus_tag="OHN27_004529"
     CDS             complement(1123..>1405)
                     /locus_tag="OHN27_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2288831.1"
                     /translation="LQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGELTRITGL
                     SASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          384 a          331 c          331 g          359 t
ORIGIN      
        1 agtttcagaa aataacagct gaaataatct cgcgcaggac tgtaaataga ttaaattttg
       61 tggaaatata ataagtgatc gcttacacta cgcgacgaaa tacttttttg ttttggcgtt
      121 aaaaggtttt ctttattatg tccgtaatgt tacaaagttt aaataacatt cgcactctcc
      181 gtgcgatggc tcgcgaattc tccattgacg ttcttgaaga aatgctcgaa aaattcaggg
      241 ttgtcactaa agaaagacgt gaagaagaag aacagcagca gcgtgaactg gctgagcgtc
      301 aggaaaaaat tagcacctgg ctggagctga tgaaggctga cggaattaac ccggaagagt
      361 tattaggtaa tagctctgct gctgcaccac gcgctggtaa aaaacgccag ccgcgtccgg
      421 cgaaatataa attcaccgat gttaacggtg aaactaaaac ctggaccggt cagggccgta
      481 caccgaagcc aattgctcag gcgctggcag aaggtaaatc tctcgacgat ttcctgatct
      541 aattactgag gccggatgtc gctgctaggg cgcgtccggc gtcgcttctc aagctcgttg
      601 gttccacggc atcttatcta acaaccttgc cattccacaa aaaccgctga ttcctgcaaa
      661 cagtaacccg gcaccaacaa agccacttaa taagaagaaa ccgctattta cggtataacc
      721 cagtacaacg ccgattaata ttaaaccacc cgcagcgatc tgcacctggc gcattaacgg
      781 caagggttga gatttattta ccgctactgg caatccagct cttttccagc catcaatccc
      841 atcttcgagt aaaaatattt ctgcgggggc ggcaatcgct gctaatttat cggcattatt
      901 actggtgcgt ttacctgcct ggcagtggaa tataatttgc tcgtgacgta atttagccgg
      961 aagacctgac tgttccagca cggataatgg agccagatct gcttcaggaa tatgttcacg
     1021 aagatattca tcagcatcac gaatatcgat taactttgcg ccgcgtgcga ttaattcttg
     1081 tgcatcatgc ggcgaaatgg ttgtcaaagc catagtgact ccttacggac aatagacatt
     1141 tttcagggtg gcgataatgg catttaccgc ctcattttta atggaatata gaatgcgttg
     1201 ggcatcccgt tggctgtcga taagcccttc gtcccgcata cgagcgagat gctgtgatgt
     1261 cgcagaggca ctcagtccgg taatgcgcgt cagctctccc gcgctggtgc cgggggaacc
     1321 gctaagcatg cacagaatca gcaatcgttt aggatggctc attgctttca ataaggccgc
     1381 tgcctgttcg gcactggcct gtaat
//
LOCUS       DAJOSY010000279         1398 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJOSY010000279 DAJOSY010000000
VERSION     DAJOSY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.282"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(65..466)
                     /gene="pemK"
                     /locus_tag="OHN27_004530"
     CDS             complement(65..466)
                     /gene="pemK"
                     /locus_tag="OHN27_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HCQ2288832.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            complement(399..656)
                     /gene="pemI"
                     /locus_tag="OHN27_004531"
     CDS             complement(399..656)
                     /gene="pemI"
                     /locus_tag="OHN27_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HCQ2288833.1"
                     /translation="MHTTRLKKVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPHRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(749..1081)
                     /locus_tag="OHN27_004532"
     CDS             complement(749..1081)
                     /locus_tag="OHN27_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCQ2288834.1"
                     /translation="MTKLFADKSIPDVSLTLLTIFILAPLNEETLFRGIMLNVFRSRY
                     CWTMWLGALITSLLFVAAHSQYKNLLTLAELFLVGVITSAARIRSGGLLLPVLLHMEA
                     TTLGLLFG"
BASE COUNT          380 a          414 c          312 g          292 t
ORIGIN      
        1 accagcagcg gttttagtgc cctaaatcga acgttttcgt ccggttgccc ctcaaacccc
       61 atgttcaagt tagaatagtg gacagacggc caagaacttc gttcatgata gtctctggaa
      121 cccgttcgag tcgttttccg ccccgggctt tcatatcaat tgtccggggt tgatcgcaac
      181 gcacaacacc tgtggtacgt atgccaacac catccagcga caccgcaaag ccggcagtgc
      241 gggcaaaatt gcctccgctg gttacgggca caacaacagg cagtcgggtc acgcgattaa
      301 aggccgccgg tgtgacaatc agcaccggcc gcgttccctg ctgctcatga cctgcggtag
      361 gatcaagcga gacaagccag atttcccctc tttccatgtc agatttcctc ctgaccagtc
      421 gccggtgcat ccagccattc tcgttcttca gctgatattt cagcattcgg atcacactgt
      481 gccagtagct cagccagtga atattgcggg cgtctgtgcg gctcaacaat cagccggcca
      541 ttatcaatga ccatgccaac ttcattatct gtacccagag acagcgcatt cagcagtgcc
      601 ggtggaacgg tcagcataac tgagccgcca accttcttca gtcgggtggt atgcattctt
      661 cacctccata aaagttatat ttaaatataa catccactaa aaaaacacac caggctttaa
      721 cgcgcaatgt ttaataaaaa tataactttc aaccaaacag taaacccagc gtggttgctt
      781 ccatatgcag caataccggc agcagcaggc caccacttct gatcctggcc gctgatgtaa
      841 tcacccccac caggaacagc tctgccagtg tcagcaggtt cttatactgg ctatgcgcgg
      901 cgacgaacaa caacgacgtt atcagcgccc ccagccacat cgtccagcag taccgtgaac
      961 ggaagacgtt cagcataatc ccccggaaca gcgtttcctc attcaacggg gcaaggataa
     1021 agatggtcag caacgtcagg ctcacgtcag gtatagactt atcggcaaaa agtttcgtca
     1081 taaatggctc agcaggcaga gccagcacct taccgagcag aaatacaccg atatacacca
     1141 cggccatcgc accgaccagc cacggtacgc caacgttgcg cagctgacca acgaccggta
     1201 gcggagctat ccaacggcgg tataccagga aaacacacag caggtacatc agaacagcac
     1261 catgactgaa gaacaaatag ttttttcctg gcccataaag cagaacggcc tgctccatga
     1321 caaatctggc tccccagcta atgccccatg cagccagcat aaccagcata aactgcagat
     1381 attgattacg tgtctgaa
//
LOCUS       DAJOSY010000280         1384 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJOSY010000280 DAJOSY010000000
VERSION     DAJOSY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.283"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(1297..>1384)
                     /locus_tag="OHN27_004533"
     CDS             complement(1297..>1384)
                     /locus_tag="OHN27_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCQ2288835.1"
                     /translation="GRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          434 a          255 c          277 g          418 t
ORIGIN      
        1 taaacaggtt agtacctatg caatttaacg gtatttttcc aagtatctat gcaatcctac
       61 acagattcta tacagtatcg acatagtctc tttttggtct aacttattga aatatataaa
      121 gaatctgtga agtctctttt cagggtaaac tgtatcgtgt tagtcgtccc tctccagaca
      181 ctacatatag tgctatatac agggctatat aacgatatgt agatagttgg tatcaaacca
      241 cttaacgatt tcctacgtga aagttagaaa gtgatagtta tagttacctt gtcaaaactt
      301 cgtagcccct tcttaaaaaa tcgtgaaagg taatcttgtt atctatacag aatctacgta
      361 gatacttctt agtgcgcaaa acagctatta tgttaaaaac acactgtata tgtatcagta
      421 ctgatttata agggttttgt gtattggctg ataagagact atacagcgcc ttgagagcat
      481 gtcttgtaag ttattgatac ataaggtcac agaagtttgt agctgttaca gagaaaactg
      541 ggcaggactt cacagagcta taaaatgtta cagccattga taggtgaaat cttcttgata
      601 gatgctttga agtgtgatga aggatttttg aggtggaatt aacagatgct atgaagaaag
      661 tcaatagttg gttatagata ttttatttcc ttttgcaaag ggggtgctat atcgtgaatc
      721 tgggctacta aacgggtata gacctgtcgc agatattttt gaagattgtc gggaaaggga
      781 actcgaagag ggtctaatcg attttataaa aaattagaag ctggatatta gttgtacaaa
      841 ttttatacaa acaaaaaagg actccggtga aggagtcctc atcaggtaga tttttaatgt
      901 ggctactacc atccacccat accgcaccaa accttacccc accgaaccgc accctacacg
      961 accttacctc gccggacact gccatagaga gtgtgcttgg ttgctttgga agtaaaccta
     1021 gtacagtaag ctattataca tatcgagata gtaacgtcaa gaaactttta acaggagggg
     1081 ttcctatggt cacatatccc ctctctttcc actaagtaag ccttagcagc acctaagcaa
     1141 caattgacta acagattcgg tagagtctat ctcataggtc aacttcataa actcagagct
     1201 aagttacctt gaaagattct accaagaact acacaggtgt tatttagtct gactgtgaag
     1261 ggatatttaa cgggagaaga gggtaatgac tccaacttat tgatagtgtt ttatgttcag
     1321 ataatgcccg atgactttgt catgcagctc caccgatttt gaaaacgaca gcgacttccg
     1381 tccc
//
LOCUS       DAJOSY010000281         1377 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJOSY010000281 DAJOSY010000000
VERSION     DAJOSY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.284"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1313
                     /locus_tag="OHN27_004534"
     CDS             <1..1313
                     /locus_tag="OHN27_004534"
                     /inference="COORDINATES: protein motif:HMM:NF015354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCQ2288836.1"
                     /translation="IKTMAIFHMSAQTITRSKGHSSVAAAAYRHGEKLIDEHTGEIHD
                     YSKKKGISDSVILIPESADRNFLKPAYLWNTIEKSEKRKDAQLAREFNIALPVEMTDE
                     QKKALAIDFCQENFVKNGMIADIAFHKLDSDNPHFHVMLTTRLLTPDGAGFGQKERKW
                     NDKELLEQWRKSWADAANEHMQKAGIDAHIDHRSLKEQKAEFDAILPPLRTVEQEAQA
                     ISLDRPPTIHRGHSRNPERQARFDVLQEVKVSQEAKALNHIEQKQLKPSPLAPASSPA
                     ASLQDATQGGNENNLQNRLGEVGEYLQSKLNKEDKADLNADNEIKVHTTKNNSKSSSA
                     AAIGSTSNINLTDEKVDKNLHLTEAQKEELEDLENKATAAKKKAYLMGGAVVPEFSQD
                     NKTQSKSEFKNKLHIAKLSISLTEQRFNEEREAYENRRNRPITR"
BASE COUNT          518 a          284 c          287 g          288 t
ORIGIN      
        1 agataaaaac aatggcaata tttcatatga gcgctcaaac aataacacgc agcaaggggc
       61 atagtagcgt agctgcggca gcttatcgcc acggcgaaaa gttaatagac gaacacacag
      121 gagagatcca cgactacagc aaaaagaaag gtatttcaga cagtgtgata ttaatacctg
      181 aaagcgcaga ccgtaatttt ttaaagcctg catatttatg gaacacgata gaaaaaagcg
      241 aaaaaagaaa agatgcgcag ttagcacgcg agtttaatat tgcgcttcct gtcgaaatga
      301 cagacgaaca aaaaaaggcg ttagcaatag atttttgcca agagaatttt gtaaaaaatg
      361 gaatgattgc tgatatagcc ttccacaaac tcgatagcga caacccgcat tttcacgtaa
      421 tgctaactac tcgcctcctt actcctgacg gcgcggggtt cggtcagaag gaaaggaaat
      481 ggaacgataa agaactgtta gagcaatggc gcaagagctg ggcagacgca gcaaacgagc
      541 acatgcaaaa ggctggcatc gatgcgcata tcgatcacag atcactgaaa gaacaaaaag
      601 cagagtttga cgctatcctg ccccctctca gaacggttga gcaagaggcg caggcaatca
      661 gcttagacag accgccgact atccatagag gccatagccg caatccagag cgacaagcac
      721 gctttgacgt gctccaagaa gtgaaagtat cacaagaggc aaaagcactt aaccacattg
      781 aacagaaaca gcttaagccc tcgccgttgg ctccagcttc ctcccctgcc gcatctttgc
      841 aagatgctac acagggaggc aatgaaaaca acctgcaaaa tagactcggc gaagttggcg
      901 aatatcttca atctaaactc aataaagaag ataaagcaga tcttaatgct gataatgaaa
      961 tcaaagttca caccactaaa aataatagta aaagcagttc agcagcggct ataggttcaa
     1021 catcaaatat taatttaacc gatgagaaag tagataagaa tctacactta acagaagcac
     1081 aaaaagaaga gttagaggat ttagaaaata aagcaacagc agcgaagaaa aaagcttatc
     1141 tgatgggcgg cgctgtcgtt cctgaatttt ctcaagataa caaaacacag tcaaaaagtg
     1201 agtttaaaaa taagcttcat atagcaaaac tatcaatatc attaactgaa cagagattta
     1261 atgaagaacg tgaagcatat gagaacagaa gaaaccgacc aataacgcgc taaatatttt
     1321 gctcattttt catccccaat gaatgacttg gagttatatt aactttaacc aattgat
//
LOCUS       DAJOSY010000282         1375 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJOSY010000282 DAJOSY010000000
VERSION     DAJOSY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.285"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1178)
                     /gene="groL"
                     /locus_tag="OHN27_004535"
     CDS             complement(<1..1178)
                     /gene="groL"
                     /locus_tag="OHN27_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313151.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCQ2288837.1"
                     /translation="MAAKDIRFGEDARARMVRGVNVLANAVKATLGPKGRNVVLEKSF
                     GAPTITKDGVSVAKEIELTDKFENMGAQMVKEVASKTSDNAGDGTTTATVLAQALIRE
                     GMKAVAAGMNPMDLKRGIDKAVTSAVEELKKISKPCSTSKEIAQVGSISANSDTDIGE
                     LIAKAMDKVGKEGVITVEEGSGLENELDVVEGMQFDRGYLSPYFINNPQSMQAELEDP
                     FILLHDKKISNVRDLLPILEGVAKAGKPLLIVAEDVEGEALATLVVNTIRGIVKVCAV
                     KAPGFGDRRKAMLEDMAILTGGTVISEEVGLSLEKATINDLGRAKKVQVSKENTTIID
                     GAGDTADIEARIKQIKAQIEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     "
     gene            complement(1234..>1375)
                     /locus_tag="OHN27_004536"
     CDS             complement(1234..>1375)
                     /locus_tag="OHN27_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCQ2288838.1"
                     /translation="DNGNVRAPKVKVGDQVLFGKYAGTEVKLDGTEYLVVKEDDIFAV
                     LG"
BASE COUNT          182 a          451 c          445 g          297 t
ORIGIN      
        1 ttcttttcct tcatctcgac ttcggtggcg gcgccgacct tgatcaccgc aacgcccccg
       61 gccagcttgg ccacgcgctc ctgcagcttc tcgcggtcgt agtccgaggt ggtctcctcg
      121 atctgcgcct tgatctgctt gatgcgggct tcgatgtccg cggtgtcgcc ggcgccgtcg
      181 atgatggtgg tgttctcctt cgagacctgc accttcttcg cgcggccgag gtcgttgatg
      241 gtcgccttct cgagcgagag gccgacttcc tcggagatca cggtgccacc ggtgaggatg
      301 gccatgtcct ccagcatcgc cttgcggcgg tcgccgaagc ccggggcctt gaccgcgcag
      361 accttcacga tgccgcggat ggtgttgacc accagcgtgg ccagcgcctc gccctcgacg
      421 tcctcggcga cgatcagcag cggcttgccg gccttggcca cgccctcgag gatcggcagc
      481 aggtcgcgga cgttcgagat cttcttgtcg tgcagcagga tgaacggatc ctccagctcg
      541 gcctgcatcg actgcgggtt gttgatgaag tacggcgaca ggtagccgcg gtcgaactgc
      601 atgccctcga cgacgtcgag ctcgttctcc aggcccgagc cctcctcgac ggtgatcacg
      661 ccttccttgc cgaccttgtc catggccttg gcgatcagct cgccgatgtc ggtgtcggag
      721 ttggccgaga tcgaaccgac ctgggcgatc tccttgctgg tcgagcaggg cttggagatc
      781 ttcttcagct cctcgacggc cgaggtgacg gccttgtcga tgccgcgctt gaggtccatc
      841 gggttcatgc cggcggccac ggccttcatg ccctcgcgga tcagggcctg ggccagcacg
      901 gtggcggtgg tggtgccgtc gccggcgttg tcggaggtct tggaagcgac ttccttgacc
      961 atctgcgcgc ccatgttctc gaacttgtcg gtcagttcga tctccttggc gacggacacg
     1021 ccgtccttgg tgatcgtcgg ggcgccgaag ctcttctcga gcacgacgtt gcggcccttc
     1081 gggcccaggg tcgccttgac ggcgttggcg agcacgttca cgccgcggac catgcgggcg
     1141 cgggcgtctt caccgaaacg aatatccttg gcagccattg cggaattacc tcagttctgt
     1201 gattgcgggt tgggatgcgg cgaagcggcg ggctcagccc agcacggcga agatgtcgtc
     1261 ttccttcacc accaggtact cggtgccgtc gagcttgacc tcggtgccgg cgtacttgcc
     1321 gaacagcacc tggtcgccga ccttgacctt cggcgcgcgc acgttgccgt tgtcg
//
LOCUS       DAJOSY010000283         1372 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJOSY010000283 DAJOSY010000000
VERSION     DAJOSY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.286"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(350..925)
                     /locus_tag="OHN27_004537"
     CDS             complement(350..925)
                     /locus_tag="OHN27_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HCQ2288839.1"
                     /translation="MSRNQEKKTPVIVVKKRRVISPPPVVDKTASVTPSVPEVVAAPA
                     PAAGQKKKGRPPRRPLREHWTREFTDQCMEKVKAIFPHLRAEGGGFLPLRVGISRDIP
                     AWLAEHPEAGLTRDEWDCAASCITSRRVYLLRTAVTGAIRYDLDGKPAGQVSEDEAKN
                     AQRWLAIRDRRWEKKQAALAGATGGENTTAK"
BASE COUNT          345 a          348 c          344 g          335 t
ORIGIN      
        1 gcattagaat cgtgtgaatg atatcccgtg ttgaccgagt tcatgtctcc ttttggatac
       61 ttaataataa tgtggccggc taacaggcaa ttgtccccca aagtaagagc atttactgat
      121 tttttgataa atgaaatgaa acaacaaaaa caggcattca aaccatataa cgatcgacaa
      181 tctccgctcc ccgggcgcgg agattgtcaa atttacggcg aagggaatca gcaccgtaaa
      241 cagtaacagc atggcgcggg caaccagcct cagcctctgg ccagccagtc aatgacaacc
      301 tgcggttcgg cggtcggaga gtgacaggat tgcccggccg ggagctgagt cattttgcag
      361 tcgtattctc cccgcctgtt gccccggcca gagcggcctg ttttttctcc caccggcggt
      421 cccggatggc cagccagcgc tgagcatttt ttgcctcatc ctcagaaacc tgcccggcag
      481 gcttaccgtc gaggtcatag cggatggcac cggtgacagc ggtacgcaac agatagacac
      541 ggcgggaggt gatacaggat gcggcacagt cccattcatc ccgggtgagc ccggcttcag
      601 ggtgttcagc cagccaggca ggaatatcgc gggagatacc gaccctgaga ggcagaaaac
      661 cgccgccttc agcgcgaaga tgcgggaaaa ttgccttgac cttttccata cactggtcgg
      721 tgaactcccg tgtccagtgt tctctgaggg gcctgcgtgg tggtcttcct ttctttttct
      781 gtccggcagc aggagccggt gctgctacca cttccggtac agacggcgtg acagacgctg
      841 ttttatcgac gacaggtggt ggtgaaataa cgcgacgttt tttcacgaca atgacgggcg
      901 ttttcttttc ctgattcctg gacataaata ccccggcgat ataataaaac cggttataaa
      961 tatatcacaa gcgcattatt atatacacca ttcaggaata tcagtccgga cagaaaatat
     1021 taatttcact gactccgtcg cgttccggaa ataaaaacgg tctgattata ctcccgacat
     1081 attgtctgcc gttgtatttt tctccgccag gggctgtaat aactttatct taaatcagca
     1141 aagagagcat tgcacagccc gcagacgttg attacgatac ccgctggtat ttgccgggat
     1201 ggtttgagta tccgccggga aaatgcgtga catcattaat ttcaacactt actgtgcgat
     1261 tgtctgttat gcctgccctc cgggcaggct tttttctgta tgcagaaaaa aatcaccgga
     1321 aaaaacggca gcagataaaa aagagtactg aatgagatat tatttcatcc gc
//
LOCUS       DAJOSY010000284         1371 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJOSY010000284 DAJOSY010000000
VERSION     DAJOSY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.287"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..539)
                     /locus_tag="OHN27_004538"
     CDS             complement(<1..539)
                     /locus_tag="OHN27_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2288840.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLY"
     gene            complement(665..1015)
                     /locus_tag="OHN27_004539"
     CDS             complement(665..1015)
                     /locus_tag="OHN27_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCQ2288841.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(1218..>1371)
                     /locus_tag="OHN27_004540"
     CDS             complement(1218..>1371)
                     /locus_tag="OHN27_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2288842.1"
                     /translation="LRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALP
                     PLTSER"
BASE COUNT          280 a          417 c          391 g          283 t
ORIGIN      
        1 tgatacaggg tttcgcgact gattccgaat tcacgagcaa gcttggtctt ttgctcgcca
       61 gcctcgacac gttggcgcag ttcggcaata cgctcagacg acagggattt cttcctgcca
      121 cggtaagccc cgcgttgctt ggcgagcgca ataccctcgc gctgacgctc gcggatcagg
      181 gcgcgctcga actcggcgaa cgcgcccatc accgagagca tcaggttcgc catcggagag
      241 tcttcgccag taaaactgag gtgttccttg acgaattcga tatgcacgcc gcgttgtgtc
      301 agcgtttgca cgatccggcg caaatcatcg agattgcgcg ccaggcgatc catgctatgc
      361 accaccacgg tgtcgccggt gcgggcgaag cttatcagcg cttccagttg cggacgcttg
      421 acatccttgc cggatgcctt gtcgctaaaa gcgcgatcaa ccttgacgcc ttccagttgc
      481 cgttccgggt tctggtcgaa ggtgctgacc ctgatatacc caatgcgctg tccagtcatg
      541 gaattccctg caaaatgtca gggaagactc tatgaccttc aacgagatat gtcaataaat
      601 tcaaaattca atcctatcct gacgcaattt acacatggca tctgacatca ggttagggta
      661 tgcctcaacc tgacggcggc gaatcacaag cgtccggttt gacgctggtt tcggtcgcag
      721 tgctggcccg cgcctggaag cgctgatagc actccagccc gcagaaatgc tccacgtact
      781 cggccccttc cggcgtgaag gcggcatcga gcgggatttc cttgcagcac acgcagcaac
      841 tggtgctcgg ttcattggcg ttcatggtgg tgttcctcca ttggttgacg aagccgacga
      901 aggccgccgc cggcatcggc ctggcgaaca ggaaaccctg caccgtgtcg caacccgcct
      961 gccgcaacca cgcaaggcag tcgggtgttt ccacaccctc ggctaccacc tccattgtcg
     1021 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
     1081 tggatccatc aggcaacgac gggctgctgc cggccatcag cggacgcagg gaggactttc
     1141 cgcaaccggc cgttcgatgc ggcaccgatg gccttcgcgc aggggtagtg aatccgccag
     1201 gattgacttg cgctgcccta cctctcacta gtgaggggcg gcagcgcatc aagcggtgag
     1261 cgcactccgg caccgccaac tttcagcaca tgcgtgtaaa tcatcgtcgt agagacgtcg
     1321 gaatggccga gcagatcctg cacggttcga atgtcgtaac cgctgcggag c
//
LOCUS       DAJOSY010000285         1332 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJOSY010000285 DAJOSY010000000
VERSION     DAJOSY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.288"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1231
                     /gene="cas3"
                     /locus_tag="OHN27_004541"
     CDS             <1..1231
                     /gene="cas3"
                     /locus_tag="OHN27_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCQ2288843.1"
                     /translation="ILLSATLPMKQKQKLLDTYGLHTDPVENNSAYPLINWRGVNGAQ
                     RFDLLAHPEQLPPRFSIQPEPIYLADMLPDLTMLERMIAAANAGAQVCLICNLVDVAQ
                     VCYQRLKELNNTQVDIDLFHARFTLNDRREKENRVISDFGKNGERNVGRILVATQVVE
                     QSLDVDFDWLITQHCPADLLFQRLGRLHRHHRKYRPAGFEIPVATILLPDGEGYGRHE
                     HIYSNVRVMWRTQQHIEELNGASLFFPDAYRQWLDSIYDDAEMDEPEWVIKGMDKFES
                     AECEKRFKARKVLQWAEEYSLQDNDETILAVTRDGEMSLPLLPYVQTSSGKQLLDGQV
                     YEDLSHEQQYEALALNRVNVPFTWKRSFSEVVDEDGLLWLEGKQNQDGWIWQGNNIVI
                     TYTRDEGMTRVIPANPK"
BASE COUNT          378 a          275 c          321 g          358 t
ORIGIN      
        1 tattcttctt tccgcaaccc taccaatgaa acaaaaacag aaacttctgg atacttatgg
       61 tctgcataca gatccagtgg aaaataactc cgcatatcca ctcattaact ggcgaggtgt
      121 gaatggtgcg caacgttttg atctgctagc tcatccagaa caactcccgc cccgcttttc
      181 gattcagcca gaacctattt atttagctga catgttacct gaccttacga tgttagagcg
      241 aatgatcgca gcggcaaacg cgggtgcaca agtctgtctt atttgcaatt tggttgacgt
      301 tgcacaagta tgctaccaac ggctaaagga gctaaataac acgcaagtag atatagattt
      361 gtttcatgca cgctttacgc tgaacgatcg tcgagaaaaa gagaatcgtg ttattagcga
      421 tttcggaaaa aatggggagc gaaatgttgg acgaatactt gtcgccacgc aagtcgtgga
      481 acaatcgctc gacgttgatt ttgattggtt aattacccag cattgtcccg cagatttgct
      541 tttccaacga ttgggccgtt tacatcgcca tcatcgcaaa tatcgtcccg ctggttttga
      601 gattcctgtt gccaccattt tgctgcctga tggcgagggt tacggacgac atgagcatat
      661 ttatagcaac gttagagtca tgtggcggac gcagcaacat attgaggaac ttaatggagc
      721 atccttattt ttccctgatg cctaccggca atggctggat agcatttacg atgatgctga
      781 aatggatgag ccagaatggg tcatcaaagg catggataag tttgaaagcg ctgagtgtga
      841 aaagaggttc aaggctcgca aggttctgca gtgggctgaa gaatatagtt tgcaggataa
      901 cgatgaaacc attcttgcgg taacgaggga tggggaaatg agcctgccat tattgcctta
      961 tgtacaaacg tcttcaggta aacaactgct cgatggccag gtctacgagg acctaagtca
     1021 tgaacagcag tatgaggcgt tagctcttaa tcgcgtcaat gtacccttca cctggaaacg
     1081 tagtttttct gaagtagtag atgaagatgg gttactttgg ctggaaggga aacagaatca
     1141 ggatggatgg atctggcagg gtaataatat tgttattacc tacactcggg atgaagggat
     1201 gaccagagtc atccctgcaa atcccaaata acttcgggaa tgattggtat caatgacgat
     1261 aacaagacca ataacggttt atccctactt aagtagggaa ggtgcacaat gtacatcttc
     1321 ttttaatttc cc
//
LOCUS       DAJOSY010000286         1310 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJOSY010000286 DAJOSY010000000
VERSION     DAJOSY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.289"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            189..1220
                     /locus_tag="OHN27_004542"
     CDS             189..1220
                     /locus_tag="OHN27_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCQ2288844.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYVGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRMAIRELRQLE
                     LTLAAPPPH"
BASE COUNT          336 a          357 c          362 g          255 t
ORIGIN      
        1 agactgaaga tgttccggaa gccataaaag gaaaaccccc actatctttc ttacgaactt
       61 ggcggaacga cgaaagatag tgggggcctc acagaatacg ggtaaagtat aatgaaaccg
      121 taccagagat tcaaccctgt gcagtgtata aatacacggc acaatcgctc cgccataagc
      181 gacagcttgt ggcaggtctg aagaatactc catataacgc agtacactgg agtcagttag
      241 cacccgaaga gcagatccgt ttctgggaag actatgaagc gggaagggcg accactttcc
      301 tggttgaacc ggaaaggaag cgcacgaagc gtcgtcgtgg tgagcactcc accaaaccca
      361 aatgcgaaaa tccgtcctgg tatcgtcctg agcgctataa ggcgctgagc gggcagctcg
      421 ggcacgccta caaccgtctg gtgaaaaagg acccggtgac cggcgagcag agcctgcgca
      481 tgcacatgtc tctgcatcct ttttacgtgc agaaacgaac gtatgtcggt cgcaaatatg
      541 ctttccgtcc ggaaaaacaa cgcctcctcg atgccgtctg gccggttctg gtcagcttca
      601 gtgatgcggg cacacatacc gtaggcatga gtgtttcccg tctggccaga gaaatcagcc
      661 cgaaagacag caaggggaag gttattccgg aactggaagt gacggtctcc cgcctttccc
      721 gtttgctggc cgaacaggta cgttttggtg tgctgggtgt ttcagaggaa accctgtggg
      781 accgtgaaac ccgccagcgt ctgccacgtt acgtctggat aacaccggca ggctggcaga
      841 tgctgggcgt cgacatggta aaacttcacg aacagcagca gaaacgactg cgtgaaagtg
      901 aaatccgcca gcagctcatt cgggaaggtg ttctgcgtga ggatgaagat atctccgtac
      961 atgcggccag aaaacgctgg tatctgcagc gcagtcagga tgcgctgaaa caccgtcgtg
     1021 cgaaagcagc agccagtaag cgcgccagac gcctgaagaa actgcctgcc gaccagcaga
     1081 ttcatgagat ggcagagtat ctcaggaagc gtctgcctcc ggatgaagcc tatttttgtt
     1141 ccgatgacca tctgaagcga atggccatca gggagttgcg tcagcttgaa ctgacgctgg
     1201 ctgccccgcc accgcactag acagcaccat tccctcagca ctgaatcatc accagcccct
     1261 ccggggcttt cggcgctggt tccgctcagc ccaaaatccg cagtaatcac
//
LOCUS       DAJOSY010000287         1296 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJOSY010000287 DAJOSY010000000
VERSION     DAJOSY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.290"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            184..1131
                     /locus_tag="OHN27_004543"
     CDS             184..1131
                     /locus_tag="OHN27_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HCQ2288845.1"
                     /translation="MYLKILATALSAPVAFAALASDTGLSFTPEKISTEIDFGTLSGK
                     AKERVYLPEEKGRKASQLDWKYSNAPIVKGAFNWDLLPRVSVGASGWTTLAGRGGNMV
                     DRDWLDTSNPGTWTDESKHPNTRLNFANEFDLNIKGWLLNQPDYQLGLMAGYQENRYS
                     FTAKGGSYIYSSEGGFRDETGSFPDGERAIGYKQHFKMPYIGLTGNYRYDSFEFGGSF
                     KYSGWVKASDNDEHYNPEKRITYRSDVNNQNYYSVSLHAGYYITPAAKVYVEGTWNRI
                     TNKKGDTSLYSRNLNISTKNGAGIESYNFVTTAGLKYYF"
BASE COUNT          402 a          233 c          294 g          367 t
ORIGIN      
        1 gcagtaatca taccgggttt gactgactcc cctgatattc tgacataaaa gggaataaat
       61 aaagcgacat gtggccacat tgtaaacaaa cgttaaacaa aacagcattt ttcagttgac
      121 tactacgagt aaataaaaaa atatttatca aaaacaaacg agtggaatgg agttaacttg
      181 tctatgtact taaagattct tgctactgca ctctcagctc ctgtagcgtt tgctgcactg
      241 gcttctgata ctgggctttc ttttacaccg gagaaaatca gtacggagat tgattttggc
      301 actttaagtg gcaaggccaa agaacgggtt tatctgcctg aagaaaaagg gcgcaaagcc
      361 agccagctgg actggaaata cagtaatgcg ccaattgtca agggggcatt taactgggat
      421 ctgttaccca gagtatctgt cggtgcctca gggtggacaa cattagctgg tcgtggaggc
      481 aatatggtgg acagggactg gctggatacg agcaatcccg gaacctggac tgatgagagt
      541 aaacatccga atacccgtct aaattttgct aatgaattcg acctgaatat taagggatgg
      601 ctcctgaatc aaccagacta tcagttaggt cttatggctg gttaccagga aaatcgttat
      661 agctttacag caaaaggagg ctcttatatc tatagtagtg agggaggctt ccgggatgaa
      721 acgggatcat ttccagatgg tgaaagagct ataggctaca aacaacactt taaaatgccc
      781 tatatagggt taaccggtaa ttatcggtat gatagctttg agttcggggg gagtttcaaa
      841 tatagcggat gggtgaaggc atcggataat gatgagcatt acaaccctga gaaaaggatc
      901 acctatcgta gcgatgtaaa taatcagaac tattactctg tctcactaca tgctggttat
      961 tatattacac ctgcagctaa agtttatgtc gaaggaacat ggaaccgtat tactaataag
     1021 aaaggtgata cttctctgta ctccagaaat ttgaatatat ccaccaaaaa tggtgcaggc
     1081 attgaaagct ataactttgt gaccacagct ggtctgaagt attattttta acctgaagca
     1141 tgcctgtttt taactttttc atgacacttt aaatatgata aacaaaaggc cagcacttct
     1201 gatggtggtc ttttgatttt ttataatatg tggagggtat atgtgctcaa cgataaaagt
     1261 gtttctgtta actcttgtat ttttaaagat gctgcc
//
LOCUS       DAJOSY010000288         1291 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJOSY010000288 DAJOSY010000000
VERSION     DAJOSY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.291"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            32..1144
                     /locus_tag="OHN27_004544"
     CDS             32..1144
                     /locus_tag="OHN27_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="HCQ2288846.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
                     AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
BASE COUNT          323 a          314 c          352 g          302 t
ORIGIN      
        1 ggtattacgc ctgatatgat ttaacgtgcc gatgaattac tctcacgata actggtcagc
       61 aattctggcc catattggta agcccgaaga actggatact tcggcacgta atgccggggc
      121 tctaacccgc cgccgcgaaa ttcgtgatgc tgcaactctg ctacgtctgg ggctggctta
      181 cggccccggg gggatgtcat tacgtgaagt cactgcatgg gctcagctcc atgacgttgc
      241 aacattatct gacgtggctc tcctgaagcg gctgcggaat gccgccgact ggtttggcat
      301 acttgccgca caaacacttg ctgtacgcgc cgcagttacg ggttgtacaa gcggaaagag
      361 attgcgtctt gtcgatggaa cagcaatcag tgcgcccggg ggcggcagcg ctgaatggcg
      421 actacatatg ggatatgatc ctcatacctg tcagttcact gattttgagc taaccgacag
      481 cagagacgct gaacggctgg accgatttgc gcaaacggca gacgagatac gcattgctga
      541 ccggggattc ggttcgcgtc ccgaatgtat ccgctcactt gcttttggag aagctgatta
      601 tatcgtccgg gttcactggc gaggattgcg ctggttaact gcagaaggaa tgcgctttga
      661 catgatgggt tttctgcgcg ggctggattg cggtaagaac ggtgaaacca ctgtaatgat
      721 aggcaattca ggtaataaaa aagccggagc tccctttccg gcacgtctca ttgccgtatc
      781 acttcctccc gaaaaagcat taatcagtaa aacccgactg ctcagcgaga atcgtcgaaa
      841 aggacgagta gttcaggcgg aaacgctgga agcagcgggc catgtgctat tgctaacatc
      901 attaccggaa gatgaatatt cagcagagca agtggctgat tgttaccgtc tgcgatggca
      961 aattgaactg gcttttaagc ggctcaaaag tttgctgcac ctggatgctt tgcgtgcaaa
     1021 ggaacctgaa ctcgcgaaag cgtggatatt tgctaatcta ctcgccgcat ttttaattga
     1081 cgacataatc cagccatcgc tggatttccc ccccagaagt gccggatccg aaaagaagaa
     1141 ctaactcgtt gtggagaata acaaaaatgg tcatctggag cttacaggtg gccattcgtg
     1201 ggacagtatc cctgacagcc tacaaaacgc aattgaagaa cgcgaggcat cgtcttaacg
     1261 aggcaccgag gcgtcgcatt cttcagatgg t
//
LOCUS       DAJOSY010000289         1261 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJOSY010000289 DAJOSY010000000
VERSION     DAJOSY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.293"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..336
                     /locus_tag="OHN27_004545"
     CDS             <1..336
                     /locus_tag="OHN27_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288847.1"
                     /translation="EGGLSVEQQQKLAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKV
                     YQNALGMRVGEQQKLKEFDLSNPLVQAKLKERYGKNIPLEETVVSPQAVFDAPQLTTV
                     AKEWPLFSW"
     gene            481..1156
                     /locus_tag="OHN27_004546"
                     /pseudo
     CDS             481..1156
                     /locus_tag="OHN27_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          331 a          311 c          301 g          318 t
ORIGIN      
        1 gaaggcggac tgagtgttga acaacagcaa aaactggcgc aaacggcaaa acgttatctc
       61 ggtaaaccgt atgatttcag cttctcatgg agcgacgatc gccagtactg ttcggaagtg
      121 gtgtggaagg tttaccagaa cgcgctggga atgcgcgtgg gcgagcagca gaagttgaaa
      181 gagttcgatc tcagcaaccc gctggtccag gctaagctca aagaacgcta cggcaaaaat
      241 attccgcttg aggaaacggt agtttcacca caagccgttt tcgacgcgcc acaactcacc
      301 acagtcgcca aagagtggcc gctgttttcg tggtaatgga tgatgaaaaa aataattccc
      361 gccgtagtct gtttgttgct ttgtagttgc acgacctttc cgcaagctgt cgctccggta
      421 aacggtgatg cctccaatta gttgaattaa ggatataata ctcgctttta aggttgtttc
      481 atggtcacag ttaaccttca ttgcccccgt tgccagtccg ttcaggttta ccgtcacggg
      541 cagaacccaa aaagacatga tcgattccgc ggccgtgact gtcaccgggt atttcagctc
      601 agttactgtt acgagccccg taaaccggga gttaaagacc aaatcgctga aatgactttc
      661 aacggtgccg gggttcgcga tactgcccgt acgctgaaaa tcggcattaa tacagtcatc
      721 cgaactttaa aaaactctcg ccacgaagaa taacgtcatc cccggtcgct catgctgatg
      781 tggcactcat ctgcaaactt agtcagcaat ggagctttgt cggcagtaaa gcgcgacagc
      841 actggctctg gtacgtgtac aacacgaaaa ctggtggtgt tctggcctac acttttggcc
      901 cccggactga cgaaacctgt cgtgaattgc gggcgctgct taccctttta ccatcggcat
      961 gttgaccact gacgaatggg gtagttacgc tagagagtta ccgaaggaga agcatctgac
     1021 tggtaagatt tttactcagc acattgagcg taacaatctg accctgagaa cccgaatcaa
     1081 acgtctggcc cgcagaacca tctgtttctc gcgttctgta gaacttcatg aaaaagtcat
     1141 cggtgctttt atcgaaaata tatgttctac taattggagt catcaccctt ctgataagct
     1201 ttccttggat gattaccaga ctgaatacac agccaaagat ttttacctca ttctgctctt
     1261 t
//
LOCUS       DAJOSY010000290         1249 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJOSY010000290 DAJOSY010000000
VERSION     DAJOSY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.294"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..539)
                     /locus_tag="OHN27_004547"
     CDS             complement(<1..539)
                     /locus_tag="OHN27_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCQ2288848.1"
                     /translation="MAVKISGVLKDGAGKPVQNCTIQLKAKRNSTTVVANTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATRATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKA"
     gene            complement(604..1203)
                     /locus_tag="OHN27_004548"
     CDS             complement(604..1203)
                     /locus_tag="OHN27_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCQ2288849.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          263 a          388 c          314 g          284 t
ORIGIN      
        1 gcctttgttg atgctgttcc tgcgctggaa gacgctgact gagccgacga cgcggcctgt
       61 ccggctgacg tgctggctgc acgtgctgag cctgcagcat cagtcgcacg ggttgccgcc
      121 tcacgggctg atgtgctggc atcgctggct gacttcttcg cggctgctgt gttctgcgcc
      181 actgcggacg cgttacgcgc cacctcttcc accatcagtt caaagcggcg cagtgcctcc
      241 ggacggacat catcctccgt catggcaccg agaaaatcat tcagcgtacc cggttgtgag
      301 tcttcataca cggtgatggt cccggcatgc gatggcggga agccttccac caacagaata
      361 acgctgtact gaccgtactc aacgtccatg ctgtaacgcc cggcttcatc cggattttct
      421 gaggccacag tgttcgccac caccgtggtg ctgttacgtt ttgctttcag ctggattgta
      481 cagttctgta ccggtttacc tgcaccgtct ttcagtacac ctgaaatctt tactgccata
      541 ttcaccccac aaaaaagccc gcctgaaccg gcgggctgtc ataacactgt gttacctggc
      601 taatcagaac ttataaccaa cacccacgat gaaaccgtca gtgcgccagt cgccactgcc
      661 ggagccttca taagcaatat caatggccac ggattcggtc gggttaaact gcacgccagc
      721 cccccacgcc agagacgtgt tgctgtggcg accgtcatca cttccggtca gcacgtcgtg
      781 cgttttcccc ttgttgtcag ttacgcggag ataatccccg gagaaagtcg acacacggct
      841 gtaagccaca cccgccatcg catacgcgct gaaccattca ttcacgcgca cagacggccc
      901 cgccatcacg ctgaaccagc ggttacgaac ggaatcttca tgccagcggg tatcgctgta
      961 acgggtcagc tggcgattct tgtctcctgc atagctgaat gaggtcacca tccccagtgt
     1021 gtccgtaaac tcataacggt atttcacgtt aatcccgttc agatcatcgc tgccgggaac
     1081 gttcgtccgg gcatgaagat accccgcgct cagcgtggac tgatgttcag acgcccatgc
     1141 aggcgcaccg gatacggcca gacagatggc tgcggacaaa attgctgcac aaactttacg
     1201 cataattacc tctcgctttt ctgcaataaa aaaggcgtca tttctgacg
//
LOCUS       DAJOSY010000291         1247 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJOSY010000291 DAJOSY010000000
VERSION     DAJOSY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.295"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            50..>1247
                     /locus_tag="OHN27_004549"
     CDS             50..>1247
                     /locus_tag="OHN27_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288850.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLAQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIFLHKEHPTPKLYKDGQYLDKQIEG
                     LFSTLLTINDNLLRAKAEIASTIIKFLEARITNLSYNDILKYQQEFQKQCYKQVKAFT
                     TLSRYNKIQTWEEMSEYQFEVFQYETLNPKKMSHTPYLKRPLPNEKDINYGVEIEIPS
                     GKRIRLS"
BASE COUNT          494 a          237 c          182 g          334 t
ORIGIN      
        1 atccaattta aacatcttag cataaaacaa atgatgaata aggaatttta tgtcaattga
       61 ctttacccca ggtataataa atacatatca cggcgatatt tataactgca caacaaatac
      121 cgataatgct aaaacgccag acacaccgaa gtggccttgc gataattggg aagaacaaca
      181 acccattaat tccacattct ctggagaagg atatatctct gatcaatatg atttagcgca
      241 acaccaactt caacaaatta atgcgtgcca cacgaacact acatatacaa atgcagacta
      301 ctcaaaagtt gtggctcaac ttgtaagtct tattactaac attgaaacga taagttcgac
      361 acaacttacg caacagacac aatcaatact taatcagatt aataacatta gatatgaaaa
      421 aaacaaaagt gctgaatgtc gaatcattgt tgtcgctaat cctaaacccg acaaggcaat
      481 cataactaaa attagcgtag aagagggaat acccataaca ttcagtgtcc aaaccatgtt
      541 ttcagatact aattttattg ctgaacaacg agctgactta ccaactaata taaaagatat
      601 tcaaagcctg tatcaaaaaa tgacgaagtt atatattgag cacagtgaaa ataaaaacag
      661 gatgaaagtc tttgccggaa ccaactttat tgatttcaat atgacaggac agaacctgtc
      721 cgggttcgtt ctaaccttgt caaggtttta tttcgaagat ctacttaata taaatttcac
      781 tgacgcaaat ttaggtgaca ctattttttt acataaagaa caccctaccc ccaaattata
      841 taaagatgga caatatcttg acaagcaaat cgaaggttta ttttcaacat tattaaccat
      901 taatgacaac cttttacgag caaaagcgga aattgcttcg acaataatta aatttttaga
      961 ggccaggata accaatcttt catacaatga catattaaaa tatcagcaag aattccaaaa
     1021 acaatgttat aagcaagtta aagcctttac gacactctct cgatacaata aaattcaaac
     1081 atgggaagaa atgtctgaat atcaattcga agtatttcaa tacgagacgc ttaacccaaa
     1141 aaaaatgtca cacacgcctt atttaaagag gccactgcct aatgaaaaag atataaatta
     1201 tggggtggaa atagaaatac catctggtaa aagaattcgc ttatcaa
//
LOCUS       DAJOSY010000292         1206 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJOSY010000292 DAJOSY010000000
VERSION     DAJOSY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.296"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..533)
                     /locus_tag="OHN27_004550"
     CDS             complement(<1..533)
                     /locus_tag="OHN27_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HCQ2288851.1"
                     /translation="MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLGEIFPLISSELAGMALMPETERLIGECLSLSSSPLNLKHPELKYNRL
                     RVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYGNAPALDKKSLYCSIHFVIGFS
                     KTCYRSLVDIEVGDVLL"
     gene            complement(533..652)
                     /locus_tag="OHN27_004551"
                     /pseudo
     CDS             complement(533..652)
                     /locus_tag="OHN27_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(665..1206)
                     /locus_tag="OHN27_004552"
                     /pseudo
     CDS             complement(665..1206)
                     /locus_tag="OHN27_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          326 a          255 c          195 g          430 t
ORIGIN      
        1 attaataaaa catcaccaac ttcaatatca acaagacttc tgtaacatgt tttactaaat
       61 ccaattacaa aatgaatgga gcaatataac gattttttgt ctaacgctgg agcgtttccg
      121 tattggcaga ctgtgacttt atctaaccaa agattcaatt cccctgtcca tattcttatt
      181 agtggttgag cagggctcaa tactccatca aatactttac ctacgcgcaa acgattatat
      241 tttagttcag gatgctttag atttaacggg gaactggata aactgaggca ttcccctatt
      301 aacctttccg tttctggcat taaagccatc ccagcgagct cagatgaaat taagggaaat
      361 atttctccaa gcaataccca tccagaccag ctgccactct cttctgctat cggtagccat
      421 ttagcactaa attcgggcat taataatgca acatcttcgc cattttcttt ccaattttga
      481 aaagtggttt caaacgtatg agtgtttcga tttactttcc tcagaccgaa cattaacttt
      541 catccttctg atcgttttcg aatttgcttt cattttcttg ctcctcataa ggctgatgtc
      601 gttgtccttg tcgttcatct tgctcagtca atacccaacg ttgtgctgta actcattatg
      661 aaaactattt aattccctca tcattgataa ggttggacat ctctttcaac atgagagtca
      721 ttgaagcttt cgaccaattt agcggaatga tagggcttgg cgcttcatgc aaagttcccg
      781 acttgtggat atagttatag ctttcgggaa gtgccatttc tggtacgctc ctgccatttg
      841 caccaatcat attctcgcct gttatttggg ccaatgatct attcataaat tgtactgagt
      901 cattcaaata ctcttctttt cttgattctt tataaacaat tgctgctgat tttgcatacc
      961 atgagtcaaa aaaccattct gcttctgttg atggaatgaa gctcttttct ctttttgcat
     1021 gcgaattttg atcggtatcg gttttaatat cattgaacca aaaatttgcc gactgataat
     1081 tatccttctc gtatcttttt ataccatacg gacctgctag ttttttaact atttttagaa
     1141 cttactcttt tcttctggtg tttatctttg aaagatttgt aggataaata acattaagta
     1201 aagccg
//
LOCUS       DAJOSY010000293         1161 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJOSY010000293 DAJOSY010000000
VERSION     DAJOSY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.297"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            65..850
                     /locus_tag="OHN27_004553"
     CDS             65..850
                     /locus_tag="OHN27_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288852.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNDKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          380 a          221 c          241 g          319 t
ORIGIN      
        1 aattagagtc cgttatcaat gctaaatatt ctaatcatta tgacaggcga gggagtgtcc
       61 aattatgaat tcaaaaaagc tttgttgcat atgtgtgtta ttctcgctgc ttgcaggatg
      121 tgcctctgaa tcttctattg atgaaaagaa gaaaaaagct caagtcacac aaagtaatat
      181 taataaaaac actccccagc aactgacaga caaagattta ttcggtaatg aaaccactct
      241 ggccgtatcc gaagaggata ttcaagctgc gcttgatgga gatgagttcc gcgttcccct
      301 taattctcct gtaatacttg ttcaatccgg caaccgcgca ccggaaacca ttatgcagga
      361 ggagatgcga aaatattata ctgtttccac attctctggt atcccggaca ggcaaaagcc
      421 tctgacttgt aacaaaaaca aagataaaaa tgagaacgag gatgttgcca gtgctgagaa
      481 tatgaactgg atgcaggcac tgcgttttgt ggctgcaaaa ggacatcaga aagcgattat
      541 tgtttaccag gatatgttgc agacaggaaa atatgactct gcgctgaaat caacagtatg
      601 gtccgactat aaaaatgaca aactcactga cgctatctcc ctgcgctacc tggtacgttt
      661 cacgctggtg gatgtggcaa caggtgagtg ggctacctgg tcgccggtga attatgaata
      721 taaagtgctt ccaccattgc ccgacaagaa tgaagccagt acgactgata tgacagagca
      781 gcaaatcatg caacttaagc aaaagaccta taaagcgatg gtaaaagatt tggttaaccg
      841 ctatcaataa taaattatat ctgccgccag gaatttctgg cggcaataat acaaaattct
      901 ttgcataaga aatttttctt gacaaagaca gtgcaagaaa ggacatggaa tccggagtta
      961 aaaactgttt gttttagaat atttaattat ttaactgctg aatcttcctt gcgagaaata
     1021 tatccattat tacatttcat gccattttta atatagattg ctcctgggaa aattcccgta
     1081 ttattaccgc aggggtgata attagtattg acatatcctg tgacaaaagg agctattaaa
     1141 ggtgctatta cgatagctat t
//
LOCUS       DAJOSY010000294         1157 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJOSY010000294 DAJOSY010000000
VERSION     DAJOSY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.298"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..663
                     /locus_tag="OHN27_004554"
     CDS             <1..663
                     /locus_tag="OHN27_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HCQ2288853.1"
                     /translation="LNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGTQGRL
                     NKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKL
                     CVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMN
                     EDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSG
                     LS"
     gene            complement(828..>1157)
                     /locus_tag="OHN27_004555"
     CDS             complement(828..>1157)
                     /locus_tag="OHN27_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2288854.1"
                     /translation="SLFATTSIGIFMGTIARSMPQLGLLVILVLLPLQMLSGGSTPRE
                     SMPQMVQDIMLTMPTTHFVSLAQAILYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFR
                     KTIGTMA"
BASE COUNT          360 a          249 c          287 g          261 t
ORIGIN      
        1 cttaacatgc tggatattaa aggaaaaatc atcacaactg atgcgatggg ttgccagaaa
       61 gatattgcag agaagataca aaaacaggga ggtgattatt tattcgcggt aaaaggaacc
      121 caggggcggc taaataaagc ctttgaggaa aaatttccgc tgaaagaatt aaataatcca
      181 gagcatgaca gttacgcaat cagtgaaaag agtcacggca gagaagaaat ccgtcttcat
      241 attgtttgcg atgtccctga tgaacttatt gatttcacgt ttgaatggaa agggctgaag
      301 aaattatgcg tggcagtctc ctttcggtcc ataatagcag aacaaaagaa agagccagaa
      361 atgacggtca gatattatat cagttctgct gatttaaccg ctgagaaatt cgccacagcg
      421 atccgaaatc actggcacgt ggagaataag ctgcactggc gtctggacgt ggtaatgaat
      481 gaagacgact gcaaaataag aagaggaaat gcagcagaat tattttcagg gatacggcac
      541 attgctatta atattttgac gaatgataag gtattcaagg cagggttaag acgtaagatg
      601 cgaaaagcag ccatggacag aaactatctg gcgtcagtcc ttgcggggag cgggctttcg
      661 taatcttgcc ctgaatacag gactgagtat caaaaagccg gttaactgaa actgtccagg
      721 ttttgggggt cagttcataa acgccttatc cggcctaaaa acaactaaaa ttcaataaat
      781 tgcaccgatg cgtagtccct ataagcttac gcatcgggca attgtattta cgccattgtc
      841 ccaatcgtct tcctgaatcg cagcagcgca atggtgaaaa acgcgccgcc aattgccatc
      901 agtgtcagga actgcggcca gacgatttcg aatcctgccc cccggtagag aatggcctgc
      961 gcgaggctaa caaagtgtgt cgtcggcatg gtcaacataa tgtcctgcac catctgcggc
     1021 atactttcgc gcggcgtgga gccaccagaa agcatttgca gcggcagcag caccagaatc
     1081 accagcagcc ccagttgcgg cattgaacgc gctatcgtcc ccataaaaat gccgattgac
     1141 gtggtggcaa acagact
//
LOCUS       DAJOSY010000295         1156 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJOSY010000295 DAJOSY010000000
VERSION     DAJOSY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.299"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(8..658)
                     /gene="tetR(A)"
                     /locus_tag="OHN27_004556"
     CDS             complement(8..658)
                     /gene="tetR(A)"
                     /locus_tag="OHN27_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCQ2288855.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            764..>1156
                     /locus_tag="OHN27_004557"
     CDS             764..>1156
                     /locus_tag="OHN27_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2288856.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERAR"
BASE COUNT          193 a          379 c          342 g          242 t
ORIGIN      
        1 gtcctgatca atcgtcaccc tttctcggtc cttcaacgtt cctgacaacg agcctccttt
       61 tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt
      121 cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc
      181 cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga
      241 agtagctgat tgtcatcagc gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga
      301 ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat
      361 ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct gaagctgcgg gcattcccga
      421 tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga atgcgtatga ttctccgcca
      481 gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg
      541 ccggctgctg aacccccaac cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga
      601 cctcgttcaa caggtccagg gcggcacgga tcactgtatt cggctgcaac tttgtcatgc
      661 ttgacacttt atcactgata aacataatat gtccaccaac ttatcagtga taaagaatcc
      721 gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca gacgtgaaac ccaacagacc
      781 cctgatcgta attctgagca ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc
      841 ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac gacgtcaccg cccactatgg
      901 cattctgctg gcgctgtatg cgttgatgca atttgcctgc gcacctgtgc tgggcgcgct
      961 gtcggatcgt ttcgggcggc ggccggtctt gctcgtctcg ctggccggcg ctgctgtcga
     1021 ctacgccatc atggcgacgg cgcctttcct ttgggttctc tatatcgggc ggatcgtggc
     1081 cggcatcacc ggggcgactg gggcggtagc cggcgcttat attgccgata tcactgatgg
     1141 cgatgagcgc gcgcgg
//
LOCUS       DAJOSY010000296         1134 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJOSY010000296 DAJOSY010000000
VERSION     DAJOSY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.300"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1134
                     /locus_tag="OHN27_004558"
     CDS             <1..>1134
                     /locus_tag="OHN27_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288857.1"
                     /translation="IRFDNNDNEYKSVINGGTIEDETHSRLFLEDWRKLYIDDKLNWK
                     ASDIIYWLFISREMECFRKFGIDFMRLCVDDGGEPILRYSHSESGETCGNIFFSKISP
                     IADQVANHLGISLRYFGTFHLNLENGHVWKSEGVFENIELSPDSYKKMATLSKRMFDI
                     FEGIHDSFYNYLSSYVLNGSHPSFFESLPVGKNVAPIYPEFVIENKSHNDGRHIEHIN
                     NYLEKISSHKFFKWLINTSIDPQLKLKSFIPLWIVDIMGYRDINKYVFTYEQPESESE
                     KIINDYALHLSEHSRLFYHDWKSLQLDDMLRWSASDTLEFIFLNADMDMHRENIVKFS
                     LFGLKHRDPVIRFWFMMILELSGKEFFSHVGDIALQVESKYNI"
BASE COUNT          380 a          150 c          219 g          385 t
ORIGIN      
        1 ttatcaggtt tgataacaat gataatgaat ataaatctgt tataaacggt ggaacaatcg
       61 aggatgaaac acattcaaga ttgtttctgg aagactggag gaagctatat atagatgaca
      121 aacttaactg gaaagcaagt gatattatat actggttgtt tattagtcga gaaatggagt
      181 gtttccgaaa atttggtatt gattttatga gactttgtgt agatgatgga ggagaaccaa
      241 tacttcgata ttctcactcc gagtcaggag aaacttgcgg taatatattc ttttcaaaaa
      301 ttagtcctat tgctgatcaa gttgccaatc atttgggaat atcactccgt tattttggaa
      361 catttcacct taatcttgaa aatggacatg tatggaagtc agaaggtgtt tttgaaaata
      421 tagagttgtc accagattct tataagaaaa tggctactct ctcaaagaga atgtttgaca
      481 tatttgaagg aattcatgac tctttttata attacttgtc cagttatgtt cttaatggaa
      541 gtcatccgtc attttttgaa tcattacctg tagggaaaaa tgttgcacct atataccctg
      601 agtttgtgat agaaaacaaa agccataacg atggtagaca tattgaacat ataaacaatt
      661 acctggaaaa aatatcgagt cataagttct ttaaatggct gattaacacc tcaatagacc
      721 ctcaattgaa attgaaaagt ttcatacctc tttggattgt tgatattatg gggtatagag
      781 atattaataa atatgttttt acatatgaac agcctgaatc agaaagtgaa aagattataa
      841 atgattatgc attacacctg tcagagcata gccgtttatt ttatcatgac tggaagtcac
      901 ttcaacttga tgatatgtta cgttggagtg ccagtgatac tcttgagttt atttttctta
      961 atgcagatat ggatatgcac agagaaaata tagttaagtt ttctttgttc ggattaaaac
     1021 acagagatcc tgttatcagg ttctggttta tgatgatact ggagttaagt ggaaaagaat
     1081 ttttctctca tgttggagat atagctttac aggtggaaag taaatataat attt
//
LOCUS       DAJOSY010000297         1111 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJOSY010000297 DAJOSY010000000
VERSION     DAJOSY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.301"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..290)
                     /locus_tag="OHN27_004559"
     CDS             complement(<1..290)
                     /locus_tag="OHN27_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCQ2288858.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRIDKPEEAKRNITRLADRKIWDRL"
     gene            639..836
                     /locus_tag="OHN27_004560"
     CDS             639..836
                     /locus_tag="OHN27_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288859.1"
                     /translation="MQGAPHTARLKKNDGTTMACGGHWHTTPAQVKTEKTARHLQAKK
                     VKFLHFFTLIYRADLAIVMIK"
     gene            870..>1111
                     /locus_tag="OHN27_004561"
     CDS             870..>1111
                     /locus_tag="OHN27_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2288860.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETA"
BASE COUNT          280 a          285 c          312 g          234 t
ORIGIN      
        1 aggcggtccc agattttccg gtcggcaaga cgggtgatat tccttttcgc ttcttccggt
       61 ttatctatgc gacgaacgga atcagtcacg cagtcttcaa acccgtaaag aaaaatatca
      121 cctgacgcat cgagcagaat cttgcgcgct gtcgccaggc attccatcgc ctgcatgaac
      181 agtgcaatac cttcgttacg ctgcgcgatg atgcggtcga tagccacaga cggaataaca
      241 tctgtcgtgc cggcagtatt caggtcagca gtgggtaaag tctgattcat cacattgtct
      301 cctcatcgtc acgactgccg ggacagccag gtcatttccc ggaggagcga agctccttct
      361 gccgtccggg tcttcgggga tttccccgca gtggaccttt ccgacggggg acgctcacca
      421 cgtcacgggg cagttgtccg ggggcagacg caaaagtttt tgacgcctgc ggcaaaaagt
      481 tttgtggctg gtacatctcc cccctggacg actgaaacgg gatgtggtca tgtatgtacc
      541 cccggaaagt ccgtctgcgg agaacaggcc gcagtgcggc ggcagacagg cagagacagc
      601 tgcgccatca ggcgcagtgg ccttacacac cgtaaggtgt gcagggagcg ccgcatacgg
      661 cgcgactgaa gaaaaacgac ggcacaacaa tggcatgtgg tggtcactgg cacaccactc
      721 ccgcacaggt gaaaacagag aaaaccgcac gacatttaca agcaaaaaaa gtgaaatttc
      781 ttcacttttt tacacttatt tatcgcgctg atttggcaat tgtgatgata aaataaagtg
      841 aagttttttc actttttcaa aggtgtttta tgaccggctg ggaactgcga atctggcgaa
      901 aaagcatgct ctggtcacgc gaaaaagcgg ccagagaatt tggcgtgacg cagagaacat
      961 ggcacgcctg ggaaaatgcg gaacaggtgg acgtcaccgt ctggaggacc acccaggcac
     1021 tttcagtccg tgacctgctg cctcacatgc aggggatgcg taaggcagat atcatcaggc
     1081 gactggaaaa tgaactgggg gaaacggcag a
//
LOCUS       DAJOSY010000298         1109 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJOSY010000298 DAJOSY010000000
VERSION     DAJOSY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.302"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            464..>1109
                     /locus_tag="OHN27_004562"
     CDS             464..>1109
                     /locus_tag="OHN27_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HCQ2288861.1"
                     /translation="MLFADTDSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEY
                     ERRKGILDTRLSELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLK
                     TARNALTRAEQQLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTS
                     AVIDNRANLNYLLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQ"
BASE COUNT          332 a          256 c          294 g          227 t
ORIGIN      
        1 tcccggtgca gaatcgctgg ggtatgattc agatggccat gaaattatgg ccgttgatat
       61 ttatgtaaac cctccacgtg tcgatgtttt tcatggtacc ccgcctgcat ggagttcctt
      121 cgggaacaaa accatctggg gcggaaacga gtgggtcgat gattccccaa cccgaagtga
      181 tatcgaaaaa agggacaagg aaatcacagc gtacaaaaac acgctcagcg tgcagcagaa
      241 agagaatgag aataagcgta ctgaagccgg aaaacgcctt tctgcggcaa ttgctgcaag
      301 ggaaaaagat gaaaacacac tgaaaacact ccgtgccgga aacgcagatg tcgctgatat
      361 tacacgacag gagttcagac tcctgcaggc agagctgaga gaatacggat tccgtactga
      421 aatcgccgga tatgatgccc tccggctgca tacagagagc cggatgctgt ttgctgatac
      481 tgattctctt cgtatatctc cccgcgaggc caggtcgtta atcgaacagg ctgaaaaacg
      541 gcagaaggat gcgcagaacg cagacaagaa ggccgctgat atgcttgctg aatacgagcg
      601 cagaaaaggt attctggaca cgcggttgtc agagctggaa aaaaatggcg gggcagccct
      661 tgccgttctt gatgcacaac aggcccgtct gctcgggcag cagacacgga atgacagggc
      721 catttcagag gcccggaata aactcagttc ggtgacggaa tcgcttaaga cggcccgtaa
      781 tgcattaacc agagctgaac aacagctgac gcaacagaaa aacacgcctg acggcaaaac
      841 gatagtttcc cctgaaaaat tcccggggcg ttcatcaaca aatcattcta ttgttgtgag
      901 tggtgatccg agatttgccg gtacgataaa aatcacaacc agcgcggtca tcgataaccg
      961 tgcaaacctg aattatcttc tgacccattc cggtctggac tataaacgca atattctgaa
     1021 tgaccggaat ccggtggtga cagaggatgt ggaaggtgac aagaaaattt ataatgctga
     1081 agttgctgaa tgggataagt tacggcaac
//
LOCUS       DAJOSY010000299         1101 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJOSY010000299 DAJOSY010000000
VERSION     DAJOSY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.303"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..527)
                     /locus_tag="OHN27_004563"
     CDS             complement(<1..527)
                     /locus_tag="OHN27_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HCQ2288862.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIFI
                     AFYIVNASNDPQLRH"
     gene            784..>1101
                     /locus_tag="OHN27_004564"
     CDS             784..>1101
                     /locus_tag="OHN27_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HCQ2288863.1"
                     /translation="MQVFSSDVYFTVVTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNIRLSDLTHFLISNLHTQNVYSTQEALTHDDID
                     ILRL"
BASE COUNT          380 a          206 c          157 g          358 t
ORIGIN      
        1 atatgtctca gctgcggatc attactggca ttaacaatat aaaaagcaat gaaaatgata
       61 gcaaaaagaa aaaaaagcat tacatactta aacagcttac tgctaaagaa atgttgtttt
      121 gtagaaataa tgttattttc aataatgcct ggcaatgaat actgccaaac ttcatttttt
      181 tcttttacag caaatttgag acctaaaact tctatttgct gattcaatgt taacgttcta
      241 tcttcaggtt cttgtgctgt taattctctg aatgttacta ccagatcagg gtcggtagta
      301 attattatct caaaactacc tgtaagctcg ccataaggaa cgactattgt gttttctggt
      361 aactcagaaa aagcatcact tcttcctaac gaaacatcat taccaatgat aacaagtaga
      421 cgcccattaa gtatttcata ctcgcatcca ttcaatggtc cgctcaacaa acgaatggca
      481 taagattcca ataagtcttg tgataagaat ttatcattat tttccatgaa taccacccgc
      541 taattccata agctctagtt tagagtagtg taacaagacg gaaatttcat gaccaaaata
      601 tgatgacaca gaataaaaaa ttataatttc gaggactatt ttaaatgcct ctatgcataa
      661 ctttatttac aatttaaggt ataaacccta ttcccccaat gttaagcaaa tataacaatt
      721 actcttattt taacttttac acgtaatatt taccctaaat gattttagag tacaggttgg
      781 catatgcaag tcttttccag tgatgtctac ttcactgtag tcactaatgc attattagca
      841 tcccaaaaag aatattacag cgatttagtt gcgttggttg atcttgggca ttcattcgtt
      901 gttattgatg agcatcaaca tcgaaatctc aaaccaaata cagaacctgt aaacatcctt
      961 ttaagcaaca atttcatcag gatcaataag aatattagat tatctgattt aacgcatttc
     1021 ctgatttcga atcttcatac acaaaatgtg tattccacac aagaagcact tacccatgat
     1081 gatatagata tcctgcggtt a
//
LOCUS       DAJOSY010000300         1101 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJOSY010000300 DAJOSY010000000
VERSION     DAJOSY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.304"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            35..>1101
                     /locus_tag="OHN27_004565"
     CDS             35..>1101
                     /locus_tag="OHN27_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCQ2288864.1"
                     /translation="MQRREDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQ
                     AAPGVRVNNKNELVIPFSNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRN
                     GQPVLFAEGYATAASLHEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHA
                     IRVNKGIVSATKAAELTGGTVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQL
                     EHIGVSTPNSNTPEIREALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSL
                     PSATQQEQPAASSSNIADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESP
                     ASPESTTASAPDEPAHPAEPP"
BASE COUNT          296 a          333 c          288 g          184 t
ORIGIN      
        1 tccccgcgca agactccacc tgaaagcgca ttcaatgcaa cgcagggagg acgcggagcg
       61 ggaactgaaa gcacagtaca accgacaggc cgcgtatgcc cgccgctatg tcaacaaatg
      121 gccgcaggcc acagcacatg aatacctgac ccggaaaggc attcaggctg caccgggggt
      181 gcgggtaaac aacaaaaatg aactggttat tcccttcagt aaccgtaacg gcgcgatacg
      241 ctcttaccag cgtattccgg tcaccggggg gaaggatgcc cgcatcctga aagattcaga
      301 gaaaaccggt aactggtttg cgctgggcac accgcgcaac ggtcagccgg tactgtttgc
      361 cgagggctat gccactgcgg cctccctgca cgaagccacg ggccttccgg tactgatgac
      421 ggtggatgga ggcaacatga ttgccgttgc cgaaaatgcc cggcaaaagt ggacgcaaag
      481 cccctttatc ttctgtgccg acaacgatca tgcaatccgg gttaacaaag gtatcgtcag
      541 tgcaacaaaa gccgcagaac tgacaggagg aacggtcatc tttcctgcgt tcaccgatgc
      601 cgaaaaagcg caggggctga ccgatttcaa tgacctggac gcaagccggg gacgggccgc
      661 ttttcagcat gtcatcaacg cccagttaga acacatcggg gtttccaccc ccaacagtaa
      721 caccccggaa atccgcgagg cactggtgat cggcaacctc gtttttacac cggtccacac
      781 tgaggaaaaa accatgactc cgacagaata tcctgaaaca tcacccgata cagggcacag
      841 ccatgaccag ggaccttctc tgccgtccgc cacgcagcag gaacaaccag ctgcttccag
      901 cagtaacatt gctgacgaaa cacagtcatt taccagtcac gccactgaaa ataacggcaa
      961 agatgagcgg cacgctgata acgttcaggc gcaaacggaa agctctgtag ttcaggactc
     1021 ccctgcagaa tcaccggcgt ccccggaaag tacaacggca tctgcccctg atgaacccgc
     1081 tcatccggcg gaacctccgg a
//
LOCUS       DAJOSY010000301         1097 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJOSY010000301 DAJOSY010000000
VERSION     DAJOSY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.305"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            498..644
                     /locus_tag="OHN27_004566"
                     /pseudo
     CDS             498..644
                     /locus_tag="OHN27_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(694..1047)
                     /gene="cmi"
                     /locus_tag="OHN27_004567"
     CDS             complement(694..1047)
                     /gene="cmi"
                     /locus_tag="OHN27_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HCQ2288865.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQFSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEGYVEYAND"
BASE COUNT          309 a          254 c          209 g          325 t
ORIGIN      
        1 aggggtccgc ctggaaaacg gagtttccca cgtcaagaga attgagttga acaagtggtt
       61 acatccatcc aagccagcta actcactaaa cgtaaaggat gtacgaaatg ttcctaccgt
      121 ccgataacca tttggcttac ttccgtacat tcccttgcgt agatgttcag aattgtacga
      181 gttcggacac caaaaccgct gttgtcccgc cattctggcg attcctgaaa gtcatttgac
      241 taaaaaatag gggctatttc gaattttctg gacactcctg acgcggagtc tgtgacttca
      301 acctgacacc ttacgctctt agcgtacgat tttttccgtt tcgtgagttg acccctttat
      361 tttccaaatt ccaaactgcc ccctacattt atacaatgtc cggcagggca tagaagggga
      421 gaaagaaaat acctgggcga agccgctgaa catcattctg gcgatttcaa taaaggcatt
      481 tgctcgcgct ctgtcattat gatgataaat acgattttaa cgccagcact caccgtggcg
      541 tcatcggaga gtcgctcaca aggctcgggg cgatgtgttc tggtaaagag taccagatac
      601 tgcttcctgg tgaaattcac attaaagaaa gtggtaagcg ataatataag ggccgggatt
      661 ccggcctttt tatgcagcct ttataacagt gtattagtca ttcgcatatt caacatatcc
      721 ctcctcacaa tggtacatgc cataatacat tctcttaccc tccggacctt cagctttaaa
      781 tgggacaaga agtgtttttt catcttcaac atctgttaaa cttctttcaa tcttttccca
      841 tgtaattttc gggatttctg taccaagaag ttgtacatcg ttgtcccagc gtttcattct
      901 ttgcaggtaa tcatttttta caaatgttct ggcctgttca tcgctgaact gatagcatgc
      961 cggaccttta tcttcagacc agaaaaaaac agcagcaaga gcaataatcg ttaaaataaa
     1021 aataaatttc atgctaatta ctttcataaa atctccacaa cttctgtcgt tcattcgata
     1081 taaaaaacat tacgaat
//
LOCUS       DAJOSY010000302         1084 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJOSY010000302 DAJOSY010000000
VERSION     DAJOSY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.306"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1084
                     /locus_tag="OHN27_004568"
     CDS             <1..>1084
                     /locus_tag="OHN27_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288866.1"
                     /translation="TYHYKVNGDTIKKTLERHIDLYFRDEIKTANLENRAPYVPSLKQ
                     FSYWNKKLFTKDFSINKKNTKKEIDLKMRALLGSVANTTVLPGDVFEIDSTVADVHLI
                     SSLNRRKVIGRPTIYTVVDRATRMIVGLHVSLYHASWRAARQALANCFMPKKEYCRLF
                     GISITDDDWPCSHIPLTLMCDNGEMIGLKPQEEMTPLTKLEFAPVGRGDRKSIVERCF
                     GILNDEVIHRLIGTTRRGKIVKGEPTPQSRACLTIQEVTSLLIREILAHNQRTYEELA
                     YINPLLIENDLVISPKNSWMISLKHGRFSARAVGADEVIARLLIPVNANITAGGIQYN
                     NLFYECDPEIASGVRVFGRTTCEARID"
BASE COUNT          353 a          189 c          238 g          304 t
ORIGIN      
        1 tacgtatcat tataaagtta acggagatac gataaagaaa acattagaga gacatattga
       61 tttgtatttt agggatgaaa ttaaaacagc aaacctagaa aatagagctc catatgttcc
      121 ttctttaaaa caattttcat actggaataa aaaactcttc actaaagatt tctcgataaa
      181 taagaagaac acgaaaaaag aaatagacct caaaatgcgg gcgcttttag gtagtgtggc
      241 taatacaact gttttgccgg gtgatgtttt cgaaatagac tcaactgtcg ctgatgtaca
      301 tcttatttcg agtttaaaca gacgaaaagt cattgggcgt cctactattt atacagtggt
      361 ggatcgtgca acaagaatga ttgttggcct tcatgtttct ttataccatg cttcatggcg
      421 agccgcccga caagcgttag caaattgctt tatgccaaaa aaagaatatt gtagattatt
      481 tggaatctct attactgatg atgattggcc ttgttctcac attccattaa cattgatgtg
      541 tgacaacggg gagatgattg gtcttaaacc tcaggaagag atgacccccc tgacaaaact
      601 tgagtttgcg ccagtcggta gaggcgacag aaaaagcatt gttgaacgct gtttcggcat
      661 tctgaatgat gaggttattc ataggcttat tggaacaaca cgaaggggaa agattgttaa
      721 aggagagcca acaccgcaat ccagggcttg tttaacgatt caggaggtca cgtctttact
      781 gattcgggag atacttgcac ataatcagag aacgtatgaa gaactggctt atatcaatcc
      841 cttactgatt gaaaatgatc tcgtaatatc accaaaaaat agttggatga taagcttaaa
      901 gcacgggaga ttcagcgcaa gagccgttgg ggccgacgaa gtgatcgcac gattgttaat
      961 ccctgtgaac gctaacatta ccgccggtgg gattcagtac aataatcttt tttatgaatg
     1021 tgatcctgaa attgcatcag gtgtcagagt atttggaaga accacttgtg aagcaagaat
     1081 agat
//
LOCUS       DAJOSY010000303         1080 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJOSY010000303 DAJOSY010000000
VERSION     DAJOSY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.307"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            72..485
                     /locus_tag="OHN27_004569"
     CDS             72..485
                     /locus_tag="OHN27_004569"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288867.1"
                     /translation="MNNLRPNPNPRKEGSADMMNRLNFYDTSFTCLHFLMIDFDNDMQ
                     EMKRKKDFHPLMLLNKHDRESLDLEIRNIIRISLASKMLNIKSEKLLSADLYFEKQDE
                     LDMGEDARIVSYVFRDFVKQRIEAAIKTIEEERSI"
     gene            482..718
                     /locus_tag="OHN27_004570"
     CDS             482..718
                     /locus_tag="OHN27_004570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288868.1"
                     /translation="MSILSLLAKAKPAVDDGGASFMGPAMGFFVAAFLVAIISSVVSS
                     AFRSAKNSADVSISNAKKYHVALIKEQYRKKGGG"
     gene            720..>1080
                     /locus_tag="OHN27_004571"
     CDS             720..>1080
                     /locus_tag="OHN27_004571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288869.1"
                     /translation="MYYLVCVVFMILFIIVCMLSVIYAAEIYQWQHYNGYKFKRWLKS
                     GSIKKDENEGKIKRQVKKMTIDYILKLLKKYNIDFDANELAKASFNIKLKYYKLILAE
                     KERIKENKLLDEGLKKKI"
BASE COUNT          424 a          121 c          224 g          311 t
ORIGIN      
        1 cttaaaagtt gaaggcgata aaagattgaa tgaatggaag ctaaaaaaca acaagaaaag
       61 gagtaaagca gatgaataac ttaagaccta acccgaaccc tcgaaaagag ggttcggcgg
      121 acatgatgaa ccgcctaaat ttctatgata cgtctttcac ttgcttgcat tttctaatga
      181 tagatttcga taatgacatg caagaaatga aaagaaaaaa agattttcat cctttgatgc
      241 ttttgaataa acacgataga gagagcttag atttagagat acgaaacatt attagaatat
      301 cacttgcaag caaaatgtta aatattaaaa gcgaaaagtt attgagcgct gatttatatt
      361 ttgaaaaaca agatgagtta gatatgggcg aagatgcgag aatagtcagt tatgtgttta
      421 gagactttgt aaaacaaaga atagaagcag cgattaaaac tattgaagaa gagagaagta
      481 tatgagcata ttatcattgt tagcaaaagc aaaaccggca gtagatgatg gcggggctag
      541 tttcatgggt ccggcgatgg ggttttttgt tgctgctttt ttagttgcta tcatttcgtc
      601 tgttgtttca agtgcttttc gttctgctaa aaactcagca gatgtgagta taagtaatgc
      661 aaaaaaatat cacgtagcac taataaaaga gcaatacaga aagaaaggtg gtgggtaata
      721 tgtattattt agtatgtgta gtttttatga ttttatttat tattgtttgt atgctttcag
      781 ttatttacgc tgctgagatc tatcaatggc agcattataa cggatataag tttaaacggt
      841 ggctaaaaag cggaagtata aaaaaagatg agaatgaagg gaaaataaaa agacaagtga
      901 agaaaatgac tatagattat atattaaagc ttcttaaaaa atacaatatt gattttgacg
      961 ctaacgagtt agcaaaagca agttttaata ttaagctaaa gtactataaa cttatattgg
     1021 cagaaaaaga aaggataaaa gaaaataaac tattagatga agggttgaag aaaaaaataa
//
LOCUS       DAJOSY010000304         1069 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJOSY010000304 DAJOSY010000000
VERSION     DAJOSY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.308"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..205
                     /gene="traP"
                     /locus_tag="OHN27_004572"
     CDS             <1..205
                     /gene="traP"
                     /locus_tag="OHN27_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HCQ2288870.1"
                     /translation="STPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTL
                     GNVVIQRIDPTTRTIITSAGTLR"
     gene            205..732
                     /gene="traQ"
                     /locus_tag="OHN27_004573"
     CDS             205..732
                     /gene="traQ"
                     /locus_tag="OHN27_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HCQ2288871.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            783..>1069
                     /locus_tag="OHN27_004574"
     CDS             783..>1069
                     /locus_tag="OHN27_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HCQ2288872.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVIAFLGGLIGLKKVGKQGG"
BASE COUNT          252 a          262 c          282 g          273 t
ORIGIN      
        1 ttccacaccg catccagcaa agaaaaaaac gtcgtctgta cttaacggaa tgaaaatcat
       61 gtcgatggaa actggtatgg cctggattcg ctggcagggt agtacctggg ctgtcagaga
      121 aggtcagacg ctgggcaatg tggtgatcca gcggattgat ccgactaccc ggaccatcat
      181 caccagtgca gggacgttac gctgatgaac atggatgccc tgaccgccat tgagaacttt
      241 gccagcagta ttttcagtgc cggaatggat ttcctgttta cctggggaga attcatcggg
      301 gtgatcagca tgattaccct gtttgcccgc gcccgttcag ccggtccggt aaaaatgtct
      361 cccggaaaat ttattgcggg gatgttaacc agctgcatgc tcgtatcact cccggcaatg
      421 attaacgccg gaggcgtaca aatgggattc agggctgact catttggccc gattgcatat
      481 gtacaacctc agacgtttgg cgctgctgct ggcgcagcaa atgcagttct gtcgctggcc
      541 aagctggccg gtgttggatt tgtaatgaac gggatatcca tctggcgcaa ggccggactc
      601 gatggacata ccgcactgag tgccagtgaa tccgtcagta agggaaacgt caaatttatt
      661 gccggcgtcc tgctggtctt tatcgatcgt gtcctcaatg ccctgctggc aagcattgga
      721 atcgtcttct gatttttttc accttcatat aaaccactga tatatccatt taaggagaaa
      781 ctatgtcacg tattcgtgtc gtagcggccc gtctgtacgc cgcatttatc cacgcacaac
      841 tgtttgtaat gtcatgccgg agcaaactga ctaagttcct gctgctcatt cccatgatgt
      901 tgctgcccaa agccgttctt gccgatggag atcttgccga tatggttcgc aatgtcgaac
      961 aaggtgccaa aacagcacag tcatcatcac tgactattgc gcagttcatc ggcgtaattg
     1021 cgtttctcgg gggcctgatt gggctgaaaa aagttgggaa acaaggggg
//
LOCUS       DAJOSY010000305         1067 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJOSY010000305 DAJOSY010000000
VERSION     DAJOSY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.309"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(221..892)
                     /gene="queE"
                     /locus_tag="OHN27_004575"
     CDS             complement(221..892)
                     /gene="queE"
                     /locus_tag="OHN27_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCQ2288873.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLAMLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          312 a          247 c          224 g          284 t
ORIGIN      
        1 aaggtaaata tttaaaataa ctctatacaa actaaaatct accaaacttt accgcaataa
       61 ttttcactcc agcgaaaaat taatgccaca gaatttgtag agaatgctaa tgattagccc
      121 tggatgggta aaatggagga gttttcagag gcgacaaaca atataaatga gtagaagtct
      181 ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata tttagatatt
      241 tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg cacaaacgtg
      301 tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta tcatcggtca
      361 gcattgccag tagttcatcc agtgcttcaa tatcgcgtac gcgccccact ggatgcttga
      421 tttcgttggc tcgctccagt gcctgtgaca acacttcata gccgccgcgc atgttcagct
      481 ttggcgatac ggtaacccag gtattcggtg tgcagcgtac ctcatgagta ccactggttt
      541 cgatctggca gctaaaaccg ttcttttcga gcagatcagt cagtggcagc aaatcatgaa
      601 tgcaaggctc accacccgta atcaccacat gccgcgcggt gtatccctgg cgaccaatga
      661 cagccagcaa atcttcactg ctcgcagccc cccacttatc actctctttg gtcttcgcca
      721 gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc
      781 accaggcaca gccaaccggg catccctgta aacgaataaa aatggcggga acgccggtaa
      841 agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc atagcattct
      901 ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat gtcttattaa
      961 cattctgtta caggcaggtt taaagaaaaa tgcgaaacat atcgttaata attaaaggga
     1021 gtaacgtatt atgtcagaag aaaataaaga aaatggattt aatcatg
//
LOCUS       DAJOSY010000306         1032 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJOSY010000306 DAJOSY010000000
VERSION     DAJOSY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.311"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1032
                     /locus_tag="OHN27_004576"
     CDS             <1..>1032
                     /locus_tag="OHN27_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCQ2288874.1"
                     /translation="CPGGVTSGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYR
                     TKTPAPVGSLGPGWKMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYDHDRFGQ
                     MTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQY
                     DAWGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQ
                     RYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWRYDERGWLTDISHIS
                     EGHRVAVHYRYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPA
                     VEWLTYGSG"
BASE COUNT          234 a          317 c          321 g          160 t
ORIGIN      
        1 gtgccccggc ggggtgacgt ccggccatcc ggtcaatccg ctgctcggtg caaaggtcct
       61 tcccggtgaa accgacatcg ccctgcccgg cccgctgccg ttcattctct cccgcaccta
      121 cagcagttac cggacaaaaa cgcccgcgcc ggtggggagc ctcggccccg gctggaaaat
      181 gcctgcggat atccgcttac agctgcgcga taacacactg atactcagtg ataacggcgg
      241 cagaagcctg tattttgagc acctgtttcc cggtgaggac ggttatgacc atgaccgctt
      301 cgggcagatg acggcggtgc accgcgagga agggctgagc cagtaccgtg catacgacag
      361 ccgtggacag ttaattgccg tgaaagacac gcagggccat gaaacgcggt atgaatataa
      421 cgccgccggt gacctgacca ccgtcattgc cccggacggc agcagaaacg ggacacagta
      481 cgatgcgtgg ggaaaggccg tccgtaccac gcagggcggg ctgacgcgca gtatggaata
      541 cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac ggcagccaca ccaccttccg
      601 ttacgatgta cttgaccggc tgatacagga aaccggcttt gacggccgca cacagcgtta
      661 tcaccacgac ctgaccggca aacttatccg cagcgaggat gagggtctgg tcacccactg
      721 gcactatgac gaagcagacc gcctcacgca ccgcaccgtg aagggtgaaa ccgcagagcg
      781 gtggcggtat gacgaacgcg gctggctgac agacatcagc catatcagcg aagggcaccg
      841 ggtggcggtg cattacaggt atgatgagaa aggccgcctg accggtgagc gccagacggt
      901 gcatcacccg cagacggaag cactgctctg gcagcatgag accagacatg cgtacaacgc
      961 gcagggactg gcgaaccgct gtataccgga cagcctgccc gccgtggaat ggctgaccta
     1021 cggcagcggc tg
//
LOCUS       DAJOSY010000307          978 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJOSY010000307 DAJOSY010000000
VERSION     DAJOSY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.312"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            83..343
                     /locus_tag="OHN27_004577"
     CDS             83..343
                     /locus_tag="OHN27_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288875.1"
                     /translation="MDEERVFSLSYEQLTRFTERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRKLIWPEDDWK"
     gene            340..930
                     /locus_tag="OHN27_004578"
     CDS             340..930
                     /locus_tag="OHN27_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288876.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLEVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQVNESVTP"
BASE COUNT          228 a          258 c          303 g          189 t
ORIGIN      
        1 atattatcat aaatcaataa cacacaaatc atgcaggatg tgatttttac ctttaagaac
       61 attatcagat ggagcgtgca ggatggatga agaaagggtg ttttcactca gctatgaaca
      121 actaacccgg tttacggaac gacgcatccg ggaatgcaat ctggacagcc agggcgcgat
      181 ttatctctgc gagtcagcca aagccggagc cgtgctgatc ttctggcatg aactggccat
      241 aaatggctat gcctcaatga atgccataaa gagacaggag ctcattgacg ccgactttca
      301 gcgcctcaga aagctcatct ggccggagga tgactggaaa tgactcagag ccgccgaccg
      361 tcgccactgc agcggagagt gctgattgtg ctggctgccc ttgatgaaaa acggccagga
      421 cccgtgctga cgcgggacat agaacgggtg ctggagcgaa gcggggaagc cccggtctac
      481 gggccaaacc tgcgcgcctc ctgccgtcgt ctggaggatg ccggctggct gcgtaccctg
      541 cgcgctccga acctgcagct ggcagtggag ctgacggacg ccggccgggc cgtggcacag
      601 cctctgctgc tggcagaaca ggaccgcctc cgggcagaac agcgtgcggc agaagtcgtc
      661 gtactgccgc tggtcccggc agcaggtctg cccgcagatg gcacttctgc cactgatctg
      721 gaggtggagc tgaacggcat aacgtatcag gcctgccgtg gggattttgt ggtgcgtctc
      781 gacggcagca cctgtctgca gttatggaat aaagaaggca gagtggtacg ccgggagggc
      841 gatccgctgg aagtggcgca gtggctgcag gcctgtcatg atgccggaat agaagttcgt
      901 gtacaggtta atgaaagcgt cactccgtaa aaacatgcca gcacggttta cacatcaccc
      961 catggtggca gccggtta
//
LOCUS       DAJOSY010000308          973 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJOSY010000308 DAJOSY010000000
VERSION     DAJOSY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.313"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            102..575
                     /gene="excA"
                     /locus_tag="OHN27_004579"
                     /pseudo
     CDS             102..575
                     /gene="excA"
                     /locus_tag="OHN27_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
     gene            complement(647..856)
                     /locus_tag="OHN27_004580"
     CDS             complement(647..856)
                     /locus_tag="OHN27_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCQ2288877.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
BASE COUNT          297 a          207 c          180 g          289 t
ORIGIN      
        1 tacctttctt tttattttgc gcactgacat ctacactgat gttatttagt tctcgttatt
       61 atggtgacaa cattgaagat tatgttgtca cttttggtag ttggttctta ttacttgcgc
      121 caggtatatg gatgtattca cgtgcaaaaa caaggagaga aaagataaga aaagttgtac
      181 aaacaataaa agagtcaggc ttctactctc cagagaaggg gtatgaagga ttatctttaa
      241 ctcagggagc ttatttcggt attgacctta aaaatggcac aatgttgtat gtccgtatct
      301 atccaggcaa cattatggat gttatcggtt ttgacattca taatttcact cgtactgtaa
      361 cagacgacaa aacactggaa attcatacga aatacatcaa cctgccaatg gttcctattc
      421 catcatggtg tactcatcca gaaaccgcat caaacaccat gcacgctatg gcaagtcgag
      481 gatatgatta ccctgtcgac tttcctcgcc tgattcagga gaagcgtaag gagtgggagc
      541 agattgcagg catgcctgta gccgaagtat tttaagataa gctccctcca taacagccgg
      601 ttcaaatgag ccggcttttg ttcattacaa cagataactt atgccgttac cgataaattc
      661 gaccaagtgc tttaatcgca gcaattttta tttccatatc atcactttga ctcaatctat
      721 gtaattcacg agtaatgttc tccgcctgac accggccttc acctaatgca tagatcgcag
      781 caagtgtaac gtcttttgat ttactcttca tcatttcaaa caagaaagta ttagctatcg
      841 caggaggcat gctcataatc aatcctcttc atgaaataaa caatggcact cacttacaaa
      901 caggagtcca ctatcgcaag cgactcaact tagcaatatc tctcaatttg aatgaaaaaa
      961 ccagatggca tgt
//
LOCUS       DAJOSY010000309          962 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJOSY010000309 DAJOSY010000000
VERSION     DAJOSY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.314"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            59..775
                     /locus_tag="OHN27_004581"
     CDS             59..775
                     /locus_tag="OHN27_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2288878.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          325 a          160 c          191 g          286 t
ORIGIN      
        1 ttaacacatg ttaacgattt accagtaatg taaataaatt ttcgaggaga tcattccagt
       61 gggacgtaaa tgggccaata ttgttgctaa aaaaacggct aaagacggtg caacgtctaa
      121 aatttatgca aaattcggtg tagaaatcta tgctgctgcc aaacaaggtg aacccgatcc
      181 agaattaaac acatctttaa aattcgttat tgaacgtgca aagcaggcac aagttccaaa
      241 gcacgttatt gataaagcaa ttgataaagc caaaggcggc ggagatgaaa cgttcgtaca
      301 gggacgttat gaaggctttg gtcctaatgg ctcaatgatt attgctgaga cattgacttc
      361 aaatgttaac cgtacgattg ctaacgttcg cacaattttc aataaaaaag gcggcaatat
      421 cggagcggca ggttctgtca gctatatgtt tgacaatacg ggtgtgattg tatttaaagg
      481 aacagaccct gaccatattt ttgaaatttt gcttgaagct gaagttgatg ttcgtgatgt
      541 aactgaagaa gaaggtaata ttgttattta tactgaacct actgaccttc ataaaggaat
      601 cgcggcgcta aaagcagctg gaatcactga gttctcaaca acagaattag aaatgattgc
      661 tcaatctgaa gttgagcttt ccccagaaga tttagaaatc tttgaagggc ttgttgatgc
      721 ccttgaagat gacgacgatg tacaaaaagt ttatcataac gttgcaaatc tctaattata
      781 tgttaaagaa atctgtcttt atggcagatt tctttaatct catataatcc ttataaaaaa
      841 tataatgttt aactcgtcat attgcttata aaaaatcccc gttcccagag aaataatatt
      901 tattaaaatg gcaggtcttc tttttctgat taccgaagac aaagtggcaa ttacgtatag
      961 tt
//
LOCUS       DAJOSY010000310          961 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJOSY010000310 DAJOSY010000000
VERSION     DAJOSY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.315"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>961)
                     /locus_tag="OHN27_004582"
     CDS             complement(<1..>961)
                     /locus_tag="OHN27_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCQ2288879.1"
                     /translation="LADNKKENAPELNVSYGIDKNFLYGAGVSISSVLINNSDVNFVF
                     HVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEY
                     LSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNG
                     RYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNIAFNMNNIYLAK
                     DFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQ
                     SPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          305 a          177 c          169 g          310 t
ORIGIN      
        1 tttcttaagc ttgtacttaa ttaatgaagt tatgcctttt atatactcac catgggcaaa
       61 cagatgctta tattgtttct gcatttctgc aacagtccgc gcctctttaa gaggatattt
      121 cttccaggga gattgttcac gcgcgatatt aaagtatgat gcagacggat atccagccca
      181 gctatgccat ggtttagtta tccctgtata gtgaatcaac actgttttat cggtaatggt
      241 ttgctgatac tttcgatgac tacgatcata aagttcgttt ttcagggtat aaatagtatc
      301 aaaatccttc gcaaggtaga tattattcat attaaatgcg atattcaacg catcctgatc
      361 taaatattta agagagccat atttagtttc ccctcgtaaa agtttgagta aatacggtgt
      421 caaatttgct tcatgccatt ttttaagatt gacatagata acgcctgcat tgaaataacg
      481 tccattcatt tcgggaatgt tgaggcgttt tgcacatgcc gcctgagtac tatcattgtc
      541 aggaatgacc gcagcaaact catctttaaa tataatttct gtgagaggtt tcaggcttcc
      601 tttacaaaca acatcggcat ccagatacag cagtgtggaa atactttcac tcagatattc
      661 aaaagacaat accctgaagt atgtcgcgta cgaccaaaac tgtgacgtcg gcagatcagc
      721 aaagtatttg gggtcaatta aatatacaat aattgaggta ttaaattgtt ttgctgtttc
      781 attaaatgac tttaaataat catcatccac ataatcagtg aaaacatgaa agacaaaatt
      841 aacatctgaa ttattaatca aaacggaaga aattgaaacg ccagcaccat acaaaaaatt
      901 cttatcgata ccgtaggaaa catttagctc tggggcgttt tcttttttat tatctgctaa
      961 a
//
LOCUS       DAJOSY010000311          952 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJOSY010000311 DAJOSY010000000
VERSION     DAJOSY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.316"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..942
                     /locus_tag="OHN27_004583"
                     /pseudo
     CDS             1..942
                     /locus_tag="OHN27_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          317 a          139 c          188 g          308 t
ORIGIN      
        1 aataaaaata atgccgatca gttttttgaa tgcgctaaaa gaaatgaata gaaccttttc
       61 gataatataa gaaaaagtga ttttcatgtt ggtttactta agccaagtag tacgcgtagt
      121 gttattttag aaacgccgcc aaatgtctgt atggaatcac gtaattcata tgaaaacaaa
      181 atagatgaga tttcatcttt gtcagagtca aaggaacacc ccatagatat tcaagaaaaa
      241 aaagatgcgt ttgtgaatga gttcaagggg gtattatttg ataaaaatac aaggtcttca
      301 gagcttctgt ttaattttta tgagtgttgc tataagtttt taccaagagc tcagcctcaa
      361 gataaaatcg atagctataa ttcagcactg caagcttttt ccattttttg ttcatctacg
      421 ttgacacata ataatatagg ctttgatttc aaattatttc ctgaagtcaa gctgtctgga
      481 gaacatcttg aaacggtatt caaatacaaa aatggcgatg atgtccggga gatagccaaa
      541 attaacatta ctctccaaaa agaagagggt ggtttatata atttacgtgg attggatttt
      601 aaggggtgct tcttttctgg acagaacttc agtaactatg atattcaata tgtgaactgg
      661 ggaacgtcat tgtttgatgt tgatactccg tgtattttta atgcgcctgc ttacaacaag
      721 agtaatgaaa aatcattaaa acctgtgagc gaaaacggtt taagtggagt attgactgat
      781 cgtaataata aaataaaact catcacgggc gtggcaccat tcgatgatat tttatttatg
      841 gatgatgact ttgatgatag ttcctctgag gatgatcccg ttgagaatag tcctgttgtg
      901 actagtcccg ttgtatcaag ttctaaaagc agttttcaat gattaataaa tg
//
LOCUS       DAJOSY010000312          917 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJOSY010000312 DAJOSY010000000
VERSION     DAJOSY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.317"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            49..>917
                     /locus_tag="OHN27_004584"
     CDS             49..>917
                     /locus_tag="OHN27_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCQ2288880.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT          286 a          195 c          209 g          227 t
ORIGIN      
        1 taaattaaaa ttaattattt aattgagaaa tggttaggga gaacctacat ggccataagc
       61 atcaagggtg ttaataccgg tgttattcgc aagagtaata atttcattgc actggcgctg
      121 aaaattaaag aacctcgcaa taaagaatcg ttgttcttta tgtctgtcat ggaactgcgc
      181 gacctgctga ttgcgctgga aagtcgtctg catcaaaagc ataaactgga tgctgctgca
      241 cgccttcagt atgagcaagc tcgcgataaa gtcattaaga aaatggcaga aaacatccca
      301 gaaattctgg ttgatgagct taaaaatgcc gatattaatc gtcgggttaa cacgctggaa
      361 cttaccgata atcaaggcga aaatcttacc ttcgtattaa cgctacatga cggtagcaaa
      421 tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg cgcgagcaat cattcatgca
      481 attaataatg cggaaatgcg tgaactggca ttacgtatta cctcactgtt agatttcctg
      541 ccgttatatg acgttgactg ccaggaaaat ggcaatctgg aatatgacac ctattcccaa
      601 ccggaatgga aacataactt gttcgaccat tatctggcgg tgctttaccg ctttaaagat
      661 gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa cgcgtgaagc aacgccgggt
      721 aaagaaatag aggcgatcac tcgccgaatg cttgatttca gcccacgact gaaaaaactc
      781 gcgggtgtgc catgtcaggt ctatgttcgc accgtagcgg cgaataacgc ccaaccgcta
      841 acccaggatc aatgcttacg cgcgctgcat cacttacgtg ttcaatccac cagcaaaact
      901 gcaccacagg cgaaata
//
LOCUS       DAJOSY010000313          907 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJOSY010000313 DAJOSY010000000
VERSION     DAJOSY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.318"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            105..>907
                     /gene="intI1"
                     /locus_tag="OHN27_004585"
     CDS             105..>907
                     /gene="intI1"
                     /locus_tag="OHN27_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCQ2288881.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQA"
BASE COUNT          169 a          267 c          277 g          194 t
ORIGIN      
        1 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
       61 cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg
      121 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
      181 acttgcatta cagcttacga accgaacagg cttatgtcca ctgggttcgt gccttcatcc
      241 gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt
      301 cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct
      361 tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag gagatcggaa
      421 gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc
      481 tcggttttct ggaaggcgag catcgtttgt tcgcccagct tctgtatgga acgggcatgc
      541 ggatcagtga gggtttgcaa ctgcgggtca aggatctgga tttcgatcac ggcacgatca
      601 tcgtgcggga gggcaagggc tccaaggatc gggccttgat gttacccgag agcttggcac
      661 ccagcctgcg cgagcagctg tcgcgtgcac gggcatggtg gctgaaggac caggccgagg
      721 gccgcagcgg cgttgcgctt cccgacgccc ttgagcggaa gtatccgcgc gccgggcatt
      781 cctggccgtg gttctgggtt tttgcgcagc acacgcattc gaccgatcca cggagcggtg
      841 tcgtgcgtcg ccatcacatg tatgaccaga cctttcagcg cgccttcaaa cgtgccgtag
      901 aacaagc
//
LOCUS       DAJOSY010000314          891 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJOSY010000314 DAJOSY010000000
VERSION     DAJOSY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.319"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            306..381
                     /locus_tag="OHN27_004586"
     tRNA            306..381
                     /locus_tag="OHN27_004586"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:339..341,aa:Ile,seq:cat)
BASE COUNT          270 a          164 c          196 g          261 t
ORIGIN      
        1 tggagtgaaa agtaacagat atcataatga tgcgaaaact cttcagctaa tgtaataaag
       61 ttatattatt agatacaaaa tcattacaaa gtatcattga atcgatgata actatagata
      121 gtgctaatta ttaacatttg cttgttgact gcgagtatcg actttgcgaa tggaggcggt
      181 gtcgggtttt atgtcgcgtg taatcacgac ccaaggtgaa tgggaacgca taaaaatctg
      241 ttatttacat catatgatca tcatgtttat tgcgtggttt ataagtattg ttattgcaca
      301 ctcaaggccc tttagctcag tggttagagc aggcgactca taatcgcttg gtcgctggtt
      361 caagtccagc aagggccacc aaatcattat ttacgtatca ggaagagtta agggtacaag
      421 gggtactcga tggcggtcta tatttcatga ctaacgccat ttcaaggaag aagtgcggtg
      481 ggtggtttcg aatgagggct ggtgttttgg tacgtggcac gccactgcaa tgtatttatc
      541 catcagtact cattcaaaag tacctggaaa aaatgcctgc tatgccaagc gggcaaattg
      601 cttacatgtg gaattaagta attaacgata aagccagagt attatcattt attatacccc
      661 ttcatttaat caggttaatt actcaaatca ctgaactatt ctctgttcgc tcactgatga
      721 taagtgagta ccacaaccaa tgtatgtaga acaatgccgt agagtaagga tgaaatgtct
      781 ctgccacgcc ttaaacatct actactgacc agaacacccg ccgcaggcag atgagttttg
      841 tgagcgttac aggagcgtgg ctatgctcat taaccaacgc tattgagata t
//
LOCUS       DAJOSY010000315          870 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJOSY010000315 DAJOSY010000000
VERSION     DAJOSY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.320"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(28..450)
                     /locus_tag="OHN27_004587"
     CDS             complement(28..450)
                     /locus_tag="OHN27_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCQ2288882.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVLALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(497..799)
                     /locus_tag="OHN27_004588"
     CDS             complement(497..799)
                     /locus_tag="OHN27_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCQ2288883.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
BASE COUNT          190 a          276 c          206 g          198 t
ORIGIN      
        1 gaatttcgcg ggtttctgtg ctgatattca tgacatgtgc tccccgtcca gcgcctgtaa
       61 cacaacgtcc attgcttcgc gttcttccct gacgaacgca tcaccgtcgc ccccgttctc
      121 actcccgacg gcatacaggc gcttcatttc actgcctgcc agcgccaaca ctttttccag
      181 catcaccgcc gggactgtca cctgacggct ggcgcgcttt tcttccatca gttcacgaac
      241 aacgccgtga acaacgctga catccgcacc atattcaaag gcatcgtcca gccgctgcat
      301 gacggtttcc agctcatcgt ctgtcagact ccagtcctgg gctatgtggc gcacatcccc
      361 acgggtcagc accacggcaa aaacgcattc actgtccggc acgtctgtat ccagtacatc
      421 gcggataatt tctttaacag tacagtacat gttctcgtct cctttacagg ggcggcattg
      481 ccgccccgtc cgttaatcag tccagtgctg cgtaaatcgc gttgcattca gggtgaaatt
      541 cgatgtgacg ccacaactgc gcatcgcgca tccggtaaag ctgggtcagt cctgcatccc
      601 cgctgtcgtg gtacagccac aactggcggt taatcacaag ggaggtaagg atgatgcctg
      661 cggcatcagc actcacggta cggtcaaacc agtttgcgcc gttcaccatg tgaaaacggt
      721 cgccatccgg catcatgtaa ccgccaccgt ccggcagtcg gatgaagtgc cagaatccgc
      781 cttcatattc cggcatcatt tgtttcgcca gcgcgtatac agacgcttcg gcatacagaa
      841 aatcctggcc aaacaggtaa ggcagaaaag
//
LOCUS       DAJOSY010000316          859 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJOSY010000316 DAJOSY010000000
VERSION     DAJOSY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.322"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>859
                     /locus_tag="OHN27_004589"
     rRNA            <1..>859
                     /locus_tag="OHN27_004589"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          229 a          182 c          273 g          175 t
ORIGIN      
        1 ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc
       61 cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt
      121 gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa
      181 tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat
      241 agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga
      301 gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg
      361 gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca
      421 acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc
      481 ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca
      541 ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg
      601 cagcgacgct tatgcgttgt tgggtagggg agcgttctgt aagcctgtga aggtgtgctg
      661 tgaggcatgc tggaggtatc agaagtgcga atgctgacat aagtaacgat aaagcgggtg
      721 aaaagcccgc tcgccggaag accaagggtt cctgtccaac gttaatcggg gcagggtgag
      781 tcgaccccta aggcgaggcc gaaaggcgta gtcgatggga aacaggttaa tattcctgta
      841 cttggtgtta ctgcgaagg
//
LOCUS       DAJOSY010000317          839 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJOSY010000317 DAJOSY010000000
VERSION     DAJOSY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.323"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>839)
                     /locus_tag="OHN27_004590"
     CDS             complement(<1..>839)
                     /locus_tag="OHN27_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCQ2288884.1"
                     /translation="SDYITETLTKLEKQTYPNFEIVIVNDGSKDNTSNVLREYGLTHS
                     RLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNYLLKMYSRQHETGGDAIY
                     CGLYGHHIKNGVTYSPINTEFNEGSLLFDFFYKKVRFHIGCLFIRKQLLEENNLFFDE
                     DLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSCRTITHYRHESFAHEKIY
                     SSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFKQLNQLVESNEKELKDCN
                     LPG"
BASE COUNT          269 a          153 c          123 g          294 t
ORIGIN      
        1 gccaggaaga ttacaatctt ttaattcttt ttcattgctt tcaactaatt gattcaattg
       61 tttaaaatca ccattaagta gaacattcca caaataacga gttttatgat aagctctatt
      121 ttgactcaat aatgtatgaa tttcttcttt ccggttacct ttgtataact gcatcacaga
      181 agagtagatt ttttcgtgcg caaatgactc atgtcgataa tgagtgatgg ttctacatga
      241 gttcattaag gaattttctc tatagttatg cttatacata taatatggaa ccgcatacat
      301 atcgcatgta attagcagtc gatagataaa atccagatct tctcctagtc gtaaatcttc
      361 atcaaaaaaa agattattct cttccagaag ttgttttctt ataaacaagc accctatatg
      421 gaatctaacc tttttataaa aaaagtcgaa aagtaaagat ccttcattaa actctgtatt
      481 tataggtgag taagtaacac cattttttat atgatggcca taaagcccac aataaatggc
      541 atctcctccc gtctcatgtt gtctggaata catcttcagc agatagttag gatctatctc
      601 atcatcatca tccataaaac atataaactg tccttgcgcc ttgcggatac ctgtattcct
      661 ggcagacgaa acaccgccat tttctttatt gataataatt aatcgagagt gggttaaccc
      721 atactctctc aaaacgtttg atgtgttgtc tttagaacca tcattaacaa taacaatttc
      781 aaaatttggg taagtttgtt tttctaattt cgttagagtt tcagtaatgt aatcagatg
//
LOCUS       DAJOSY010000318          822 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJOSY010000318 DAJOSY010000000
VERSION     DAJOSY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.324"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>822
                     /locus_tag="OHN27_004591"
     CDS             <1..>822
                     /locus_tag="OHN27_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2288885.1"
                     /translation="WIRVTAPHCALNLNVHFHMLWLDGVYEDTTERPQRKPRLHRTRA
                     PTSAQLTELAGTIAHRVCRHLSRRGWLEGEDESVFLSDSAGSDDGMDGLRMSSMTYRI
                     ATGRDAGRKVVTLQTLPGDAGSLEGDAGKVGGFSLHAGVAAEAHESHKLEKLCRYITR
                     PAISEQRLSISPQGRVRYQLKTPWRNGTTHVEWDAVDFIAKLAALVPPPRAHLTRFHG
                     VFAPNANLRAQLTPSGRGRRPAGDAVPVDASAHDEPRSPEQKRRAMSWAQRLKRVF"
BASE COUNT          145 a          280 c          278 g          119 t
ORIGIN      
        1 tggatcaggg tcaccgcgcc gcattgcgcg ctgaacctga atgttcactt ccacatgctg
       61 tggctcgacg gcgtgtacga ggacaccacc gagcgtccgc agcgcaagcc gcgcctgcac
      121 cgcacccgtg cgcccacatc ggcgcaactg acggaactgg ccggcaccat cgcgcatcgc
      181 gtgtgccggc acctgtcgcg ccgcggctgg ctcgaaggcg aagacgaatc cgtgttcctg
      241 tccgacagcg cgggtagcga cgacggcatg gatgggctgc ggatgagttc gatgacctac
      301 cgcatcgcca ccggtcgcga cgctggccgc aaggtcgtca cgctgcaaac attgcccggt
      361 gacgcaggct cgctggaggg cgacgccggc aaggtcggcg gcttctcgct gcatgccggc
      421 gtggccgcgg aagcacacga aagccacaag ctcgaaaagc tgtgccgcta catcacgcgc
      481 ccggcgatca gcgagcagcg gctgtcgatc tcaccgcagg gtagggtgcg gtatcagctc
      541 aagacgccgt ggcgaaatgg gaccacgcat gtcgaatggg atgcggtgga cttcatcgcc
      601 aagctggcgg cactggtccc gccacctcgc gcgcatctca cccgcttcca cggcgtattc
      661 gccccgaatg caaacctgcg tgcgcagctg acgccctcgg ggcgcggcag gcggcctgcg
      721 ggcgatgcgg tgccggttga cgcaagcgcc cacgacgagc cgcgcagccc cgagcagaag
      781 cgccgtgcga tgagctgggc gcaacggctc aagcgggtct tt
//
LOCUS       DAJOSY010000319          819 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJOSY010000319 DAJOSY010000000
VERSION     DAJOSY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.325"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(141..683)
                     /locus_tag="OHN27_004592"
     CDS             complement(141..683)
                     /locus_tag="OHN27_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2288886.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          262 a          164 c          140 g          253 t
ORIGIN      
        1 caattccggc atgtgcagga aggaccatag aaaaaatgag taaagataat aatcctttca
       61 ttttaatgcc tcttaaatta aaaggccgcc atgggatagc ggccatgttt attacctgga
      121 gaaacttaca attttattta ttaatcctgc gccacacgca taaccagaga agataacaca
      181 tcacctgatt gcactgagtt atctgtcagt ttcaggtatt ttgctttaaa cggaatagca
      241 tattgacctt ccgctgtttt ataactctca atgttaaaag gtgcagaaac atccaggaca
      301 tcgctgttat cacctaaata gagatagtaa cccacggtat cgttgctact ggctatcgcc
      361 ttatcgttac cggttatttt atcgttggta atcaacgaga gttttacacc agcaacttct
      421 ttcgggcagc taatttttaa catgctgctg gttacaccga ctgcatcatc ataggtagaa
      481 tatttagctt taccttcaaa gacagtggtt ggtacagtcg gcatattaat acttactgaa
      541 tcaccacccg cttcgaattt acacggagat tctgcaatat tccccttaat agttaatgtt
      601 ccggtatcaa cgtctgcagc aaatactcca gcgctcataa aaaaagaaga aaagacagcg
      661 gcagcaataa ttgaacgttt cataatatgt tccttaataa aatttatttt tggttgttaa
      721 gagtctgtat ctcttggaaa caaatttctc acatcaaaaa agttgccgca atgtaaacac
      781 attgttttat tgatttaaaa taaattaacc attgcaata
//
LOCUS       DAJOSY010000320          819 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJOSY010000320 DAJOSY010000000
VERSION     DAJOSY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.326"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..295)
                     /locus_tag="OHN27_004593"
     CDS             complement(<1..295)
                     /locus_tag="OHN27_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCQ2288887.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGID"
     gene            complement(396..656)
                     /gene="iraP"
                     /locus_tag="OHN27_004594"
     CDS             complement(396..656)
                     /gene="iraP"
                     /locus_tag="OHN27_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCQ2288888.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          224 a          193 c          160 g          242 t
ORIGIN      
        1 catcgatacc tttaaaaaag ccgcccgcac cttcggcata attgcgtgcg gcagtaattt
       61 ccgagtcccc cataccatcg ccaatcagca aaataatatt ttttgcaggt ttatcgctaa
      121 gagaatcacg cagagcggcg gtctgatcac ccgttaagcg gcgagcaccg ccgggtgcag
      181 taatatcgcc ctgagcagcc cggttttcca gaacaggcat ttctggtgtc cgggcttttg
      241 tcacaggggt aaacagtaac ggtaagagtg ccagtgcaat agtgctttgt ttcactttat
      301 tttctccatg tacaaataca ttaaaaaata aaaacaaagc gactataagt ctcggccgtg
      361 acaactttat gacagctgtt gaaaagatta actttttact gacgaggatg cttcaataac
      421 ttctttacgt aatcgcgcag cagctccgta tcgtcgtcag gaatgctggc atcgggcttt
      481 acctcgtaca gcgccccctc tacctgatca atcaaccgct gttggtcatt ttgcgccata
      541 ttgcgaagca ttgcagtgac gataatctcc aaagcttcta cctgcgcaca cagttctttc
      601 gactcttctt ctttttgggc aagcttaaat aacaactcag caatgagatt tttcatgtct
      661 gtatttcctt atccaaagta tggagaagtt aacattatgt ttcacaactt catagtgtct
      721 ggaagtatta tttttatgac aaaggaaata ctttgcgcaa atattttttg tttgattacc
      781 agcgaaacgt ttctcttatt taaataactg cccacacaa
//
LOCUS       DAJOSY010000321          798 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJOSY010000321 DAJOSY010000000
VERSION     DAJOSY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.327"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..745
                     /locus_tag="OHN27_004595"
     CDS             <1..745
                     /locus_tag="OHN27_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HCQ2288889.1"
                     /translation="VGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDE
                     TTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDIL
                     AGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSP
                     PDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQ
                     EEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          192 a          203 c          222 g          181 t
ORIGIN      
        1 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
       61 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
      121 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      181 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      241 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      301 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      361 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      421 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      481 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
      541 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
      601 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
      661 agatcgcttg gcctcccgcg cagatcagtt ggaagaattt gttcactacg tgaaaggcga
      721 gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac gccgcttcgc
      781 ggcgcggctt aactcaag
//
LOCUS       DAJOSY010000322          791 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJOSY010000322 DAJOSY010000000
VERSION     DAJOSY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.328"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>791
                     /locus_tag="OHN27_004596"
     CDS             <1..>791
                     /locus_tag="OHN27_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCQ2288890.1"
                     /translation="GLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADG
                     SVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTS
                     ATGPDGLEIRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWS
                     RYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHET
                     RYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEY"
BASE COUNT          199 a          225 c          244 g          123 t
ORIGIN      
        1 ggcttaagct acacgtatca gtatgagaaa gaccacatca ccatcaccga cagcctggac
       61 cgccgtgaag tgctgcacac gcagggcgaa gccgggctga agcgggtggt gaaaaaggaa
      121 cacgcggacg gcagcgtcac gcagagtcag tttgacgccg tgggcaggct caggacacag
      181 acggatgccg caggccggac aacagaatac agcccggatg tggtgacggg cctcatcacg
      241 cgcatcacca cgccggatgg cagggcatcg gcgttttact ataaccacca cagccagtta
      301 acgtcagcca ccgggcctga cgggctggaa atacgccggg aatatgatga attgggccgt
      361 ctgattcagg aaactgcccc tgacggcgat atcacccgct accgttatga taatccacac
      421 agtgacttac cctgcgcaac ggaagatgcc accggcagcc ggaaaaccat gacgtggagc
      481 cgttacggtc agttgctgag cttcaccgac tgttccggtt atgtaacccg ttatgaccat
      541 gaccgcttcg ggcagatgac ggcggtgcac cgcgaggaag ggctgagtca gtaccgcgca
      601 tacgacagcc gtggacagtt aattgccgtg aaagacacgc agggccatga aacgcggtat
      661 gaatataacg ccgccggtga cctgaccgcc gtcattgccc cggacggcag cagaaacggg
      721 acacagtacg atgcgtgggg aaaggccgtc cgtaccacgc agggcgggct gacgcgcagt
      781 atggaatacg a
//
LOCUS       DAJOSY010000323          788 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJOSY010000323 DAJOSY010000000
VERSION     DAJOSY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.329"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>788
                     /locus_tag="OHN27_004597"
     CDS             <1..>788
                     /locus_tag="OHN27_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288891.1"
                     /translation="NDNADIDSFIKLNDVKNNYWKAFKLSQEDGWVPPDLIVNLANVL
                     KKQYRISESMDYYEQAISIDNSIPQAWVNRSEALELLNELSTTYTYKMIKEVIRGYEC
                     AIKSGKCFPSWKVALQRKIKYNESILSESCVYGIEDISTDERLTQEEYDTLSNYQRFC
                     IDNNLMLSDHSLYCNCYASSKDNITIATTEEGVFGDFIVPMEMVLNRLKAEYSLARKM
                     FFEYKVDSEFFDEDSEVCYSELYNGEILYENVEKLRVAFRACFG"
BASE COUNT          277 a           88 c          166 g          257 t
ORIGIN      
        1 taatgacaat gcagatattg atagtttcat taagctgaat gatgtaaaaa ataattactg
       61 gaaagcgttt aaactttcac aagaagatgg atgggtgccc cctgatttaa tagttaatct
      121 agctaatgta ttaaaaaaac aatatcgtat atctgaatca atggattatt atgaacaagc
      181 aatatctatt gataactcaa ttcctcaggc gtgggtaaac agatctgagg cattagaatt
      241 gcttaatgaa ttatctacaa catatacata taaaatgatt aaagaagtta ttcgaggtta
      301 tgagtgtgca ataaagtcag ggaaatgttt tccttcatgg aaggttgctc ttcaaagaaa
      361 aataaagtac aacgaaagta tattgagtga atcatgtgta tatggcatag aggatataag
      421 tacagatgaa aggctcacgc aggaggagta tgatacttta tcaaactacc agaggttttg
      481 tatagataat aatctaatgt tgtctgatca ttcgctatat tgcaattgtt atgcgtcatc
      541 taaagataat ataactattg ctaccaccga ggaaggtgtg tttggtgatt ttatagtgcc
      601 aatggaaatg gtgttaaata gacttaaagc tgagtattcc ttagcacgaa aaatgttttt
      661 tgaatataag gttgatagtg agttttttga tgaggattcg gaggtatgtt attctgaatt
      721 gtataatgga gaaatattgt atgaaaatgt tgagaagtta agggttgcat ttagagcttg
      781 ctttggta
//
LOCUS       DAJOSY010000324          787 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJOSY010000324 DAJOSY010000000
VERSION     DAJOSY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.330"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            5..>787
                     /locus_tag="OHN27_004598"
     CDS             5..>787
                     /locus_tag="OHN27_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCQ2288892.1"
                     /translation="MDNQIRNISSSHQDKHSDKVNSHQHHGKVDKTHRAKIVEFDKLD
                     NDSQIDNDFGLHIIYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNTTDT
                     FIFTIIPDNNHVLKLSSPITVTVECKGGYYFINSSGDKSDIIYKVDGLSIIARNFFTL
                     LSGNFKPDWRWDVSKETFTKEKFDSYVRSVFSKIDFYKQCGVINPQNANTAYFGDTDG
                     RVGAVLYALLVSGHIGIREKGWSLLCELLKHEEMASSAYKHKN"
BASE COUNT          264 a          126 c          153 g          244 t
ORIGIN      
        1 agcaattgac aatcagatta gaaacatctc ttcaagccat caagataagc attcagataa
       61 agttaacagt catcaacacc atggcaaggt tgataaaaca catcgtgcaa agatagttga
      121 atttgataaa ctggataatg attcgcaaat agataacgat tttggcctgc atatcatcta
      181 tttcctgcaa catggtcact ggaaggtaaa tgatcgtagt caccagatgg agaaagtatg
      241 gttttacaat agtgaacctt ctatagatat tcaagaatat aatagattcg ccgacaatac
      301 tactgatacg tttatcttta caattatacc agacaataac catgtgttaa aattatcgtc
      361 tcccattacc gttactgttg agtgtaaggg cggttattat tttattaatt cctctggaga
      421 taaatcagat ataatttata aagttgatgg gctttctatc atagcgagaa atttctttac
      481 tcttcttagt ggtaatttca aacctgactg gcgttgggat gtctctaagg agacgtttac
      541 taaggaaaag tttgatagct atgttagatc tgtgttttca aaaatagact tctataaaca
      601 gtgtggtgtg attaacccac aaaatgccaa cacggcttat tttggcgata cagatggaag
      661 agtaggcgct gtactttatg cattgctcgt ttcagggcat ataggcatca gggaaaaggg
      721 ttggagttta ttatgtgaat tattaaaaca cgaagagatg gcttcatctg catacaaaca
      781 taaaaat
//
LOCUS       DAJOSY010000325          775 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJOSY010000325 DAJOSY010000000
VERSION     DAJOSY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.331"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..144
                     /locus_tag="OHN27_004599"
     CDS             <1..144
                     /locus_tag="OHN27_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCQ2288893.1"
                     /translation="YTQRIERHNLNLRQHLARLGRKLLSFSKSVELHDKVIGHYLNIK
                     HYQ"
     gene            complement(164..229)
                     /locus_tag="OHN27_004600"
                     /pseudo
     CDS             complement(164..229)
                     /locus_tag="OHN27_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
     gene            462..566
                     /locus_tag="OHN27_004601"
                     /pseudo
     CDS             462..566
                     /locus_tag="OHN27_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          256 a          156 c          158 g          205 t
ORIGIN      
        1 tacactcagc gcattgagcg acataatctg aatctgagac aacatctggc aaggctggga
       61 cggaagttac tgtcgttctc aaaatcggtg gagttgcatg acaaggtcat cgggcattat
      121 ctgaacataa aacactatca gtaagttgga gtcattacct cctcccgaac tcaaagctat
      181 catacagata attactggtt aaccctatat agggcatttt aaagtgttgt gatcttaccc
      241 agcaatagtg gacacgcggc taagtgagta aactctcagt cagaggtgat tcacaagaca
      301 aaacagtttc aaccagtaaa aaaacgtaaa caccattcgc atgaatttcg tagttaagat
      361 ctgaagcttg cagaatgcat cggtgttact gccggagtcc gtgaacttag tctgcatgaa
      421 tcaccacagc tttacatctg tcgcagtaaa cagcaaaatc agcaaactta cttaattata
      481 tcgagtgcga ttacaatcgc tggcgatggt acagttggtg tggcggtctc agtccggaac
      541 aatttgaaaa tcagaatctc acttaaaacg gcatccattt tacaaaagat ggccgtctgt
      601 gtatttgtaa tgaatacctt ctacatgttt agagtgcata atccacgaca aggcgatatc
      661 ctaaaaatta gctagcaggc aactttgatg gcatagataa tgtttttagt aaaaaagagt
      721 tgactgaata taatacagcc tcttcctaca taagaaggaa tgtgctcaca aaaaa
//
LOCUS       DAJOSY010000326          765 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJOSY010000326 DAJOSY010000000
VERSION     DAJOSY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.332"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            103..339
                     /locus_tag="OHN27_004602"
     CDS             103..339
                     /locus_tag="OHN27_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCQ2288894.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLHTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            382..654
                     /locus_tag="OHN27_004603"
     CDS             382..654
                     /locus_tag="OHN27_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCQ2288895.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            683..>765
                     /gene="ariR"
                     /locus_tag="OHN27_004604"
     CDS             683..>765
                     /gene="ariR"
                     /locus_tag="OHN27_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCQ2288896.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLL"
BASE COUNT          260 a          125 c          156 g          224 t
ORIGIN      
        1 taattgcaaa aaattaattt atcattctgt acacatattt cgtacaagtt tgctattgtt
       61 acttcactta acattgatta acatttttaa cagaggcgta gcatgcatca aaattcagtg
      121 actttagatt ctgcgggagc aatcactcgt tactttgcaa aagctaactt gcatactcag
      181 caggaaactc tcggggaaat tgtgactgag attttgaaag atggacgtaa tctgagtcga
      241 aagtcgcttt gtgccaaact tctttgccga ctggaacacg cgactgggga agaggaacag
      301 aaacattata atgcactaat tgggttgctt tttgaataat gcaacagcct gattatttaa
      361 caggttagct atgaagtcgt tatgaagaca tctgataatg aacgtataaa atatgaaatt
      421 actggccagg cggtgctcca gatactgcgc atgaagataa atttttcatt gcagactctt
      481 attaagcaat tactcgtaat gaaatcggct gaggaagatg ctttccgacg cgatttaatt
      541 gacagcataa ttcgtgattt tagcaatagt gattcaggag ggccaaaccg aagaacagcg
      601 acagctgaca ataaaagtat gttcaatggt aagaaaataa acagaataca ttaaaatttc
      661 ataagtaaga tgagaggtta ccatgcttga agatactaca attcataatg caataactga
      721 taaagcgtta gcaagttact ttcgcagttc gggtaatttg ttaga
//
LOCUS       DAJOSY010000327          764 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJOSY010000327 DAJOSY010000000
VERSION     DAJOSY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.333"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            130..>764
                     /gene="istB"
                     /locus_tag="OHN27_004605"
     CDS             130..>764
                     /gene="istB"
                     /locus_tag="OHN27_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family helper ATPase
                     IstB"
                     /protein_id="HCQ2288897.1"
                     /translation="MMQHEGHVRILKSLKLFGMAHAIEELGNQNSPAFNQALPMLDSL
                     IKAEVAEREVRSVNYQLRVAKFPVYRDLVGFDFSQSLVNEATVKQLHRCDFMEQAQNV
                     VLIGGPGTGKTHLATAIGTQAVMHLNRRVCFFSTVDLVNALEQEKSSGRQGQIANRLL
                     YADLVILDELGYLPFSQTGGALLFHLLSKLYEKTSVILTTNLSFSEWSRVFG"
BASE COUNT          195 a          182 c          207 g          180 t
ORIGIN      
        1 atgtgcttaa tctgttggga cgtttgaccg aagaacctcc acccaaaccg attccaattc
       61 ccaaggggtt aaggctgaca ttggaaccac aggccaacgt gaaccgctat gacagtttaa
      121 ggagagccca tgatgcagca tgaaggccat gtgagaatcc tcaaatcctt gaaactcttt
      181 ggcatggcac acgccattga ggagttgggc aatcagaatt caccagcatt taatcaagcc
      241 ttgcccatgc tggacagctt gattaaagct gaagtggcag agcgtgaagt acgttcggtg
      301 aactatcaat tgcgggtggc caagttcccc gtgtatcggg acttggtggg ctttgacttc
      361 agtcaaagcc tggttaatga ggccacggtc aaacaattgc accggtgcga cttcatggaa
      421 caagcccaga acgtggtgct gattggtggg ccaggcacag gcaagactca cctggccaca
      481 gccattggta cacaagcagt gatgcacttg aaccgacggg tgtgtttctt ctccaccgtg
      541 gatttggtca atgcactgga gcaagagaaa tcatctgggc gtcagggaca aatcgcaaac
      601 cgtctgttgt atgccgattt ggtgattctg gatgagctgg gatatttgcc ttttagccaa
      661 accggtgggg cactgctgtt tcacctgctc tcaaagctgt acgaaaaaac cagcgtgata
      721 ctgaccacca acttgagctt ctcggaatgg agccgagtgt ttgg
//
LOCUS       DAJOSY010000328          763 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJOSY010000328 DAJOSY010000000
VERSION     DAJOSY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.334"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..505)
                     /gene="traN"
                     /locus_tag="OHN27_004606"
     CDS             complement(<1..505)
                     /gene="traN"
                     /locus_tag="OHN27_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HCQ2288898.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENM"
     gene            complement(516..>763)
                     /locus_tag="OHN27_004607"
     CDS             complement(516..>763)
                     /locus_tag="OHN27_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HCQ2288899.1"
                     /translation="EKMNLTATTGAGVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSK
                     PEQSFVFEITIQRVDPRLKPSGMEIRQMISRNAGPNS"
BASE COUNT          157 a          185 c          229 g          192 t
ORIGIN      
        1 ccatgttctc gttgtactgc acatcaaaga gttttggtgc cgcgttataa ggaggatcgg
       61 attgtggcca tggtgaaccg ttgatatcgg taaatgtcac tacgctcagg ttgttcatgg
      121 ctgtgcgaac aagaggaagg ctggcaccgg gggacagatt aactgtcaga gagctgatac
      181 ggggtacaac actggtgatg ggcgcattga tggcccgctc gttatccgcc atgagactgc
      241 gtagtgagcg aatctcatcg gcggagagct cagaatcaag tcttgccgca tcctgtacca
      301 gcgggctggc ctgcccgggg gcaggcagct caccttttac cagtgttgcc ggaacacctc
      361 cggtgttttg ggaggcgttc tgaacgtgat cgctggcaga atttgtcgtt tgtggtgtgc
      421 gtgcgttctg ccatcctgcg ttatcggcag acgtggcggc gctgcacacc aggagcagtg
      481 tgctcagtaa ataacgtgat tgcatattac ttatctcagg agttcggtcc ggcgttacgg
      541 gagatcatct gccggatttc cataccggat ggtttcagtc tggggtcaac acgctggatg
      601 gtgatttcga aaacaaaaga ctgttctggc ttgctggttg tctggccgac cagacgcata
      661 cggacaggat actgaaatgt ccagaaccat acgccgtcgg acatttgtcc ctgtcgtacc
      721 agaacgccag cgcctgtcgt ggcggtcaga ttcatcttct ctt
//
LOCUS       DAJOSY010000329          761 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJOSY010000329 DAJOSY010000000
VERSION     DAJOSY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.335"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>761
                     /locus_tag="OHN27_004608"
     CDS             <1..>761
                     /locus_tag="OHN27_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HCQ2288900.1"
                     /translation="KYYYIQLRGAFLSAIHHQIIENQLDTEKITISYEFILCQHLIAN
                     TEFSYLALPENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAE
                     KLGGDAIKGYDQILNEKTAGIAIATASILLTKRYNVDTYTEINSYLGKLRGQQKLLDG
                     IDIIEIIYIKRPSKDLANLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNV
                     PLNYNVRHKLSLDDGGTNDFENLVLIENEPYHKVFT"
BASE COUNT          296 a          126 c          125 g          214 t
ORIGIN      
        1 aagtattatt atatacaact aagaggcgct tttttatcgg ctattcatca ccagatcatt
       61 gaaaaccaac tggatacaga aaaaataact attagttatg aatttatcct ctgtcaacat
      121 cttatcgcaa ataccgagtt cagctatttg gcactccctg aaaattataa ccgtttgttt
      181 ttaccaaatt caaaaaacca aacaaataat cgtttcaaaa cgttaaacag caaagctatt
      241 ggcaggttac ttgctgctgg tggcgtatac aatgggaaca ttgaaggatt cagagatact
      301 gcggaaaaac tgggtggaga tgcaataaaa ggctatgatc aaatactaaa tgaaaaaaca
      361 gcgggcatag cgatagcaac agcatctatt cttttaacaa agcgttataa tgttgataca
      421 tatacagaaa taaatagtta cttaggcaaa cttagaggtc aacaaaaact tcttgatggt
      481 atagacataa tagaaataat atacattaag agaccttcaa aagacttagc taacttacga
      541 aaggagttta ataaaactgt aagaaaaaat tttcttatca aacttgcaaa aacctccgaa
      601 gcatctggaa gattcaacgc cgaagacctt ttaagaatga gaaagggcaa tgttcctcta
      661 aattataatg ttcgccataa actatctcta gatgatggtg gtactaatga tttcgaaaat
      721 ttagtattaa tcgaaaacga accatatcat aaagttttta c
//
LOCUS       DAJOSY010000330          761 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJOSY010000330 DAJOSY010000000
VERSION     DAJOSY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.336"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(386..>761)
                     /locus_tag="OHN27_004609"
     CDS             complement(386..>761)
                     /locus_tag="OHN27_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288901.1"
                     /translation="SDEKRKLKINKINNKGLLGLFSIAMDLNDKNGELAFFKEWRNDL
                     EHKLLVIHEKDMLVDLYNSYGFFDEVRFIEKEKFEKQLLQLMKIVRSAVILFVFTIRI
                     EGKKSIPKDMLTIKNTIERKVL"
BASE COUNT          245 a          165 c          108 g          243 t
ORIGIN      
        1 agtaaattgc ggtggatttt gaccatttta ccccagagtg cgcgctcgta tccccgccac
       61 gcctgcccgc tttatgtagt ggttttcatg cacctgcatg ttctacgtaa aagcccgcta
      121 gttctggcga gccttagcaa aaacgatcct taaacgatca tgcgatttca tgcagcatag
      181 tcatgcactg tctaagagaa gtgaaagtcc gtatctgaat gaccacttga aaaacgaaac
      241 atacagactt acaaagatga agctctactg tgagcgagga acatacatag ttgctctgta
      301 atagccaaat tttaagcagg aaatctcttt tttatgacaa aactataaag aaaaacagac
      361 cgtttcatcg ctcgatgaaa atatactaca atacttttct ctcgatggta tttttaatgg
      421 tcaacatatc tttaggaata ctttttttcc cctctatacg tatggtaaac acaaaaagaa
      481 taacggctga tctcacaatt ttcatgagtt gtaagagctg tttctcaaac ttttctttct
      541 caataaacct cacctcatca aaaaacccat aagaattata taaatcaact aacatgtctt
      601 tctcgtgaat aactaacagt ttatgttcta aatcattcct ccattcttta aaaaaagcaa
      661 gctcaccatt tttatcattc aaatccatag caatactaaa caaacccagc aatcctttat
      721 tatttatttt gtttatttta agctttcttt tttcatcact a
//
LOCUS       DAJOSY010000331          744 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJOSY010000331 DAJOSY010000000
VERSION     DAJOSY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.337"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..744
                     /locus_tag="OHN27_004610"
                     /pseudo
     CDS             1..744
                     /locus_tag="OHN27_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          241 a          131 c          171 g          201 t
ORIGIN      
        1 cccgattaca gataagcctg gaaagtagag cataaattgt caggcatcct actattgact
       61 atttttgccg ttatttctgg tgcagaaagt tgggaagata tagaggattt cggggaaaca
      121 catctcgatt tcttgaagca atatggtgat tttgaaaatg gtattcctgt tcacgatact
      181 attgccagag ttgtatcctg tatcagtcct gcaaaatttc acgagtgctt tattaactgg
      241 atgcgtgatt gccattcatc aaatgataaa gacgtcattg caattgatgg aaaaacgctc
      301 cggcactctt atgacaagag tcgccgcagg ggagcgattc atgtcattag tgcgttctca
      361 acaatgcaca gtctggtcat cgggcagatc aagacggata agaaatccaa tgagatcaca
      421 gctatccctg aacttcttaa catgctggat attaaaggaa aaatcatcac aactgatgcg
      481 atgggttgcc agaaagatat tgcagagaag atacaaaaac agggaggtga ttatttattc
      541 gctgtaaaag gaaaccaggg gcggcttaat aaagcctttg aggaaaaatt tccgctgaaa
      601 gaattaaata atccagagca tgacagttac gcaatgagtg aaaagagtca cggcagagaa
      661 gaaatccgtc ttcatattgt ttgcgatgtc cctgatgaac ttattgattt cacgtttgaa
      721 tggaaaggac tgaagaaatt atgc
//
LOCUS       DAJOSY010000332          742 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJOSY010000332 DAJOSY010000000
VERSION     DAJOSY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.338"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..494
                     /locus_tag="OHN27_004611"
     CDS             <1..494
                     /locus_tag="OHN27_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCQ2288902.1"
                     /translation="GRLGNDPEVRYIPNGGAVANLQVATSESWRDKQTGEMREQTEWH
                     RVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEILVKTTGTVQMLGR
                     APQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQPPEGEDYGFSDD
                     IPF"
     gene            552..>742
                     /locus_tag="OHN27_004612"
     CDS             552..>742
                     /locus_tag="OHN27_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCQ2288903.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAG"
BASE COUNT          174 a          196 c          244 g          128 t
ORIGIN      
        1 tcgggcgtct gggcaatgat ccggaagtcc gttacatccc caacgggggc gcagtggcaa
       61 acctgcaggt ggccacatca gaaagctggc gtgacaaaca gacgggggag atgcgggagc
      121 agacggaatg gcaccgcgtg gtgctgttcg gcaagctcgc ggaagtggca ggtgaatatc
      181 tgcgcaaggg tgcgcaggtc tacatcgaag gtcagcttcg cacccgtagc tgggaagata
      241 acggtatcac ccgttacgtc actgaaattc ttgttaagac cacgggcacc gtgcagatgc
      301 tgggacgtgc accacagcag aacgctcagg cgcaaccgaa gcctcagcag aatgggcagc
      361 cacagagtgc tgacgcgacg aaaaaaggtg gcgcgaaaac gaaaggccgt ggacgtaagg
      421 ccgcgcagcc agagcctcag ccgcaaccgc cggaggggga ggattacggg ttttcagacg
      481 atatcccgtt ctgactggcc tgactgtgat aaccgccccg ccctgcgcgg ggcatcaccg
      541 gagagatgag gatgagcgaa tatttcagaa tacttcaggg actgccggac ggctccttta
      601 cccgcgaaca ggcggaagcc gttgccgcac agtaccggaa cgtctttatc gaggatgatc
      661 agggaacgca ttttcgcctg gttgtccgtc aggatggcac gttgatctgg cgctcctgga
      721 attttgagga ctgtgccggg ta
//
LOCUS       DAJOSY010000333          732 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJOSY010000333 DAJOSY010000000
VERSION     DAJOSY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.339"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            177..>732
                     /locus_tag="OHN27_004613"
     CDS             177..>732
                     /locus_tag="OHN27_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCQ2288904.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFFAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQEQEVEIKSGDATHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCK"
BASE COUNT          235 a          124 c          134 g          239 t
ORIGIN      
        1 ttataaattc ctaaaaatga agtgtctcta ttgtgaaaat tgctatattt cagtaagtta
       61 atttttcgct tttaaattta atattaacta aaaatacttt ttcatgttga cttacaataa
      121 atatacacat ctaatgcact aacttttttg aatcaatatt gtaagatcaa atctatatgg
      181 tggcgaaaat taatctctta tcacggctta cgccgctttt attcgtgttc gctccttttt
      241 ttgcacagtc aaatatgact gtttatccta tggctgtttc cattaatagt cagggggaag
      301 gtaacgttcg cgtaatatct aaaagtaatg aagttcaata tataaaagcg acggtatttc
      361 gtattgataa tccttcaacg cctcaagagc aggaagttga aataaaatcc ggagatgcca
      421 ctcatcttgt agtgatgccg cctaagtttg cgctgccagc gggtagcagt aagactgtgc
      481 gctttgttgc aatggaacca gagcaaaaag aaaaaaatta tcgcgttaaa tttgaagcgg
      541 ttcccagtat tgatgacgtt gccacggata aaaaagatct ctctatgcag ttaacagtta
      601 acttaatttg ggggattgtt gttagtgtcc cacctcagca acctattgct aagttagaag
      661 taaatgctgc ccaaaaatta gttaatgcag ggaatcaacg cttaaagatt ttaacgatcg
      721 cttattgtaa aa
//
LOCUS       DAJOSY010000334          728 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJOSY010000334 DAJOSY010000000
VERSION     DAJOSY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.340"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(83..703)
                     /locus_tag="OHN27_004614"
     CDS             complement(83..703)
                     /locus_tag="OHN27_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HCQ2288905.1"
                     /translation="MPLFDTPLNGINMVLKRVEDIFVSSCILLLISPLLILISIVIKL
                     TSSGPVIFKQTRYGIDGKPIKVWKFRSMNVMENDSVVTQAKKNDSRITPFGSFLRKTS
                     LDELPQFINVLVGNMSIVGPRPHAVAHNEQYRSLIEGYMLRHKVKPGITGLAQVKGWR
                     GETDTLEKMQKRVEYDLEYIREWSIWLDIKIILLTIFKGFVNKSAY"
BASE COUNT          235 a          143 c           98 g          252 t
ORIGIN      
        1 acatgcgttt gtgttttttg tctggagcat ataataaaat ttccacaagg ttcataaaat
       61 ttatcatatt aacttttgta agttaatacg cggatttatt tacaaaaccc ttgaaaattg
      121 taagtaatat aattttaata tcgagccaaa tactccattc tcgaatgtac tctaaatcat
      181 attcaactcg cttttgcatt ttctctaatg tatccgtttc accgcgccag ccttttactt
      241 gcgccagtcc agtaattcct ggtttcacct tatgccgtaa catatagcct tcaatcagtg
      301 acctatattg ttcattatga gcgacagcat gaggccgagg cccaacaata gacatattac
      361 cgactaatac attgataaat tgaggtaact catctaacga tgtttttcgt aaaaaactac
      421 caaatggagt gatccttgaa tcgttctttt ttgcttgagt gacaacacta tcattttcca
      481 taacattcat tgaccgaaat ttccaaactt ttattggttt accatctata ccatatcttg
      541 tttgtttaaa aatcactggc cctgaagatg ttaatttaat aacaatagaa attaatatta
      601 acaaaggaga aattaacaat aaaatacaac ttgagacgaa tatatcttct actctcttta
      661 aaaccatatt tattccattt agtggagtat cgaaaagagg cactaccggt actccattta
      721 cttcttcc
//
LOCUS       DAJOSY010000335          719 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJOSY010000335 DAJOSY010000000
VERSION     DAJOSY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.341"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            170..346
                     /locus_tag="OHN27_004615"
     CDS             170..346
                     /locus_tag="OHN27_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288906.1"
                     /translation="MNLVDAFVKKVISGPYKEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(411..707)
                     /locus_tag="OHN27_004616"
     CDS             complement(411..707)
                     /locus_tag="OHN27_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288907.1"
                     /translation="MSALRHVLSGILFSGKNRLQVLAKTSGGAGFGALVGEELSITDT
                     IETVVTGSVRANNGVLSPVMEKRMINLLIIVLRAVVAVANALIAVLELIRELID"
BASE COUNT          228 a          160 c          150 g          181 t
ORIGIN      
        1 ccaagataaa acatgaatac cggtatctca atgcgaagaa ataatctccg tagaaacccc
       61 catgagaaaa atatcatatg ggagaaaatc gcaattatca cagccagcgc attcattatt
      121 ttcgctgtgc tctggcatta attattttca acacaaaaag gaaaaaacaa tgaatctggt
      181 agatgccttt gtcaaaaaag taatatccgg gccatataaa gaatatggaa aatggtggat
      241 agatgttgaa tacatttcat ggggcgttcc cgggaaaaca agactgatgt ttgagtcaaa
      301 ggagcaggca ctggaagtta aagaagggta caaattttta acgtgagatc tctttttgtt
      361 aaagggcagg aagcggtaac tccctgccct tagttacgtt tccaggctta tcagtcgata
      421 agttcccgga tcagttccag aacagcaatc agcgcgtttg caacggcaac tactgccctg
      481 agaacgatga ttaacagatt aatcatccgc ttctccatca ctggagaaag aaccccgttg
      541 ttcgctctta cactgcctgt cacaacggtt tctatagtgt cagttataga cagttcttct
      601 ccgaccagag caccaaaacc agcaccacct gatgttttgg ccaggacttg taacctgttc
      661 ttcccgctga acaatatgcc ggacagaaca tgcctcagag ctgacatgcc gagtataca
//
LOCUS       DAJOSY010000336          718 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJOSY010000336 DAJOSY010000000
VERSION     DAJOSY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.342"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..718
                     /locus_tag="OHN27_004617"
                     /pseudo
     CDS             1..718
                     /locus_tag="OHN27_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT          249 a          122 c          133 g          214 t
ORIGIN      
        1 agacatgatc ttgaatgagt tgcatcaaca tgttttttta aaggacgatg cgataacccc
       61 ttgtatcttc cttggggatc atacaggcga tagatttagt actatttttg gtgataaata
      121 tatccttact ttgctaaatt ctatgtgaaa tatggagggt aataaagata gccatattaa
      181 taaaaatgtc gtcgttctgg ctggaaatca tgaaatcaat tttaatggta actatactgc
      241 acgactagca aatcataaac tctctgccgg tgatacgtat aacttaatta aaacgctcga
      301 tgtttgtaat tatgactcag aaaggcaagt tttaaccagt catcacggaa taataagaga
      361 tgaagaaaaa aaatgttatt gtctcggtgc tttgcaggtt ccatttaatc agatgaaaaa
      421 tcctactgat cctgaggaat tagccaatat ttttaataaa aaacataaag aacacatgga
      481 tgatcctcta ttccatctaa tccgaagcaa tactttgaaa tccacacctg tatatgctaa
      541 ttattttgat aacactactg atttcagacc agcacgtgaa cgtatcttca tatgtgggga
      601 gacactcaaa ggtgaagacc ccagcaaata cattagacaa aaatatgggc atcatgggcc
      661 cggggtagat cagaatcaac aatttgataa tggtattatg gggctaaatt ccctgaag
//
LOCUS       DAJOSY010000337          716 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJOSY010000337 DAJOSY010000000
VERSION     DAJOSY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.343"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>716)
                     /locus_tag="OHN27_004618"
     CDS             complement(<1..>716)
                     /locus_tag="OHN27_004618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288908.1"
                     /translation="ISLLLYIGFSQHNLKNLTQQFFYYGNNKISNNTDVSSFFDLIKL
                     EKKKYKIYFIVEPVFLGAEPTFERLSLSVEKEPPEEFSQHVFFRNLQRKKIVCVSNIE
                     AFDSYSARENAENLLKLASSFLNIYHHKDKPTWSNEAFVITDTDSLKVAERLNPMKKC
                     KDLKHEKAKKRLESLMSEFSLENSSFAKFLRSIQLHSMALKSENVENQLLNLWIALES
                     LVPTDTKSKDQATIEHITAS"
BASE COUNT          228 a          108 c          114 g          266 t
ORIGIN      
        1 tacttgcagt aatatgttct attgttgctt gatccttgga tttagtgtct gtcggcacta
       61 atgattcaag agctatccat agatttaaaa gctgattttc aacattttca ctttttaatg
      121 ccattgaatg taattgaata cttctcaaga acttggcaaa tgaactattt tctagagaaa
      181 actcagacat caatgattca agacgtttct tggccttttc atgctttaag tccttgcatt
      241 ttttcatggg gttaagtctt tcagccactt tcaaagagtc tgtatcagtt ataacgaaag
      301 cttcatttga ccaagtaggt ttatctttat gatgataaat atttaaaaag gatgatgcta
      361 atttaagtag gttttcagca ttctctcttg ctgaataaga atcaaaagct tctatatttg
      421 atacgcaaac tatcttcttt ctttgcagat ttctaaaaaa aacatgttgg gaaaattctt
      481 ctggtggttc tttctcaaca gacaaagaga gtctttcgaa agttggctct gcgcctaaaa
      541 acactggttc aacaataaaa tatattttgt atttcttttt ttcgagtttt attaaatcaa
      601 aaaaagaaga gacatcagtg ttatttgata ttttattatt cccataataa aaaaattgct
      661 gggttaaatt cttaagatta tgttggctaa agccaatgta gagtaataaa ctgata
//
LOCUS       DAJOSY010000338          710 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJOSY010000338 DAJOSY010000000
VERSION     DAJOSY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.344"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..116)
                     /locus_tag="OHN27_004619"
     CDS             complement(<1..116)
                     /locus_tag="OHN27_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCQ2288909.1"
                     /translation="METVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            78..>710
                     /locus_tag="OHN27_004620"
     CDS             78..>710
                     /locus_tag="OHN27_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2288910.1"
                     /translation="MAGAHEFRQHGFHARRRAEIISPLAQSETVGHEAADMAAQALGL
                     SRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELLQKRFLTKQKR
                     SLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAARDLQGVGGEP
                     PAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLGMVV"
BASE COUNT          128 a          224 c          234 g          124 t
ORIGIN      
        1 ggcatagctg accttgccag gcctgcttcg ccctgtagtg acgcgatcaa cgggcaggaa
       61 acattcccct ttcgtgcatg gcaggcgcac acgagttcag acagcacggt ttccatgcgc
      121 gccgtcgtgc ggagatcatc agtccgttgg cgcagtcgga gacggtcggg cacgaagcgg
      181 ccgatatggc ggctcaggcg ctgggcttgt ctcggcgcca ggtatacgtt ctgatccggc
      241 gtgcccggca aggcagcggc ctcgtgacgg atctggtgcc cggccagtcc ggtggaggta
      301 aaggtaaggg gcgcttgccg gaaccggtcg agcgcgtcat ccacgagcta ctgcaaaagc
      361 ggttcctgac caagcagaag cgcagcctag cggcctttca ccgcgaagtc actcaggtgt
      421 gcaaggctca aaaactgcga gtgccggcgc gcaataccgt ggccttacgg atcgctagcc
      481 ttgacccgcg caaggtcatc cgccggcggg aaggccagga tgccgctcgt gacctacaag
      541 gtgtgggcgg cgagcctcct gccgtgaccg cgccgctgga gcaggtgcag atagaccata
      601 cggtcatcga cctgatcgtg gtcgatgacc gcgaccggca acctattggc cgcccgtacc
      661 tgaccctcgc catcgacgtg ttcacccgct gcgtgctcgg catggtcgtc
//
LOCUS       DAJOSY010000339          702 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJOSY010000339 DAJOSY010000000
VERSION     DAJOSY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.345"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            144..368
                     /locus_tag="OHN27_004621"
     CDS             144..368
                     /locus_tag="OHN27_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288911.1"
                     /translation="MELAKDSPDAKQELLERLLSGKRMTGRDEMDIKRILKTKEALDN
                     IRNFIDKQQLTNSDTPKKSTVPNEDKKGIR"
     gene            368..634
                     /locus_tag="OHN27_004622"
     CDS             368..634
                     /locus_tag="OHN27_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288912.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
BASE COUNT          251 a          121 c          152 g          178 t
ORIGIN      
        1 tatacctgta tttctgtata gttgacaatg agtttctcag aatcggtttc tgtattgcat
       61 ggacagcata ctactttctt tcgcttttca agtattttgt aaaatacttc tttcctctcg
      121 atcgtgaaga tttgcttaaa ctgatggagc tcgccaaaga ttctcctgat gcgaaacagg
      181 agcttcttga gcgtcttttg tccggaaaaa gaatgaccgg gcgggatgaa atggatatca
      241 agcgcattct aaaaacaaag gaagcacttg ataacatcag gaattttatt gataaacagc
      301 agttaacgaa tagtgatact ccgaagaaaa gcacggtccc aaatgaagat aaaaaaggca
      361 taagatgatg atcacaggaa atggaataaa cactgtaact gtcaatggaa aagtgaaaca
      421 catcacagaa ctggatgata taacactttg tttagagtgg acaaaactac gagaggaaaa
      481 caatcgtctt tatgaaatta ataaccaggc aaacagagga tggcgtggtt tcatcctaag
      541 attgataggg gttaacctgc cagataaaag gactgagttt acacaacgtc tcttattgac
      601 gcgcaaaatt tcgggttcgg tcatgaaaaa gtaacaaaac cgcgcaacag acataaatat
      661 gacagtagcc atgaaaaagc agagagagac agggtgatac ag
//
LOCUS       DAJOSY010000340          701 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJOSY010000340 DAJOSY010000000
VERSION     DAJOSY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.346"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..580
                     /locus_tag="OHN27_004623"
     CDS             <1..580
                     /locus_tag="OHN27_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288913.1"
                     /translation="GSRSSLVAMVIAFFFSIEKKISIKTLLIYIISPIMVIAVLILFQ
                     DRIATIDGGIEQIDRVKFFNEFLYSTADWPWWQFFTGAKALTPLLPASCADLSYYQTL
                     FSYAGDGRCYSVILHSFIMRVIYDHGIIGFAFLIFCLFIYLDKYSIKEKIAILLVLIA
                     TGLSVSSLNNVYVSLALVIFVGANYSRRRLNE"
BASE COUNT          204 a          108 c          124 g          265 t
ORIGIN      
        1 aggttctcgc tcgtcattag ttgccatggt tatcgcgttt ttcttttcta tagagaaaaa
       61 aatatcaatc aaaacactgt tgatttatat catatctcca attatggtaa ttgctgtatt
      121 gattcttttt caagatagaa tcgcaactat tgatggcggt attgaacaaa tagatagggt
      181 caaatttttt aatgaatttc tctattctac agcagattgg ccttggtggc agttctttac
      241 tggtgcaaaa gcacttaccc ctctcttacc agcttcttgt gctgatctgt catattatca
      301 aactttattt agttatgctg gcgatggaag atgctattcg gtgattcttc attcttttat
      361 catgcgagta atctatgatc acggcataat aggttttgct ttcctcattt tttgtttatt
      421 catttatctt gataaatatt caattaagga aaaaatagct attctattag tgttaattgc
      481 tactggacta tcggtatcat ctctcaataa tgtgtatgta tctttagcgc ttgttatttt
      541 tgttggggca aattatagta ggagaaggtt gaatgaataa tttcgagcac ccccttttta
      601 cagtagtgat acctaattac aaacgagttg aagagcttaa aagagcttta caaagcataa
      661 tagatcaaaa aaactttgag gagttggttg ataaaattat t
//
LOCUS       DAJOSY010000341          695 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJOSY010000341 DAJOSY010000000
VERSION     DAJOSY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.347"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>695
                     /locus_tag="OHN27_004624"
     CDS             <1..>695
                     /locus_tag="OHN27_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCQ2288914.1"
                     /translation="FEVNNNQGRFVFPDTWFGPLLGEFEEVLDAYDTDEISETGYINK
                     LRRLAQQEPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSGEIIWMH
                     PENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSELLRTGDH
                     KQAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEMLCGNLHP
                     FPLAVRHNFSGSL"
BASE COUNT          176 a          160 c          168 g          191 t
ORIGIN      
        1 tttgaagtga acaataacca gggtcgtttc gtatttccgg atacctggtt tggcccatta
       61 ctgggggagt ttgaagaagt gcttgatgca tacgacactg atgaaatcag tgagacgggt
      121 tatatcaaca aactcagacg ccttgctcaa caggagcctg actttattga tatacacgct
      181 catctggcat atgcatttct ggagcagaat gccccgcgta aggcactgaa cgccgctctt
      241 aaaggactgg cggcaggtaa tcgtctcatc ccggaaagtt tcagtggtga aatcatctgg
      301 atgcatcccg aaaaccggcc ttatctgcgc gcactttatg cagcaattct cgcaaatgtg
      361 cacttacagc gtcatcagga tgcggttatg cttactgata aaatactggc atacaatcct
      421 gaagataatc agggtgcccg ctggttgttg ggctcagaac tgttacgaac aggcgatcat
      481 aagcaagcct tcagtgtact gaaagagcat gctgatgaat tttcacctta ctggtatgaa
      541 cttggtcttc tgcattttct gaatggggag catgttaagg ctgccacagc ttttcgtcat
      601 ggctttgcta caaacactta catcgcggaa atgctgtgcg gaaatttgca tccatttcca
      661 cttgctgtgc ggcataactt ctccggctct ctgga
//
LOCUS       DAJOSY010000342          686 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJOSY010000342 DAJOSY010000000
VERSION     DAJOSY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.348"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>686
                     /locus_tag="OHN27_004625"
     rRNA            <1..>686
                     /locus_tag="OHN27_004625"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          155 c          226 g          130 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaacc
//
LOCUS       DAJOSY010000343          686 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJOSY010000343 DAJOSY010000000
VERSION     DAJOSY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.349"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(447..>686)
                     /locus_tag="OHN27_004626"
     CDS             complement(447..>686)
                     /locus_tag="OHN27_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2288915.1"
                     /translation="YLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPIS
                     EIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          213 a          142 c          119 g          212 t
ORIGIN      
        1 tggatgaata tttgtagtgc agtgacatca tttttaaata atagttcaaa taaaagggct
       61 cacgatgaaa aaattaacag tggcaatttc tgctgtagct gcatcttaac gtataaacta
      121 gatttaataa caacgaacta gatttaataa caacgatatc cgtacctatc cggatatcta
      181 atgtcataag catcccttta attatcccct caacctcttg taactttccc actcttgaac
      241 tccacatagt ccaaacgccg gatgcaaaaa aatcacacgt gcatttctga aattgtgatg
      301 gaggaagata gtatcaatta tatgtgctta taagcgtccc cgattagcgc agaagtcacc
      361 ggattgttca ggctccaata ggttgagtat gccgtattta ttttccaaaa taaaaatcga
      421 tgctgttgct ccttcataaa atacttttac tctctggacg cttttcttag ctctcttggt
      481 gttatcttaa aacgattatg gaacctttca gtaaaacgag aaggacactt gtaaccattt
      541 tccctggcaa tctcgctgat gggttttact gtcgtttgta tagcagacaa cgcattattt
      601 aacctcacgt cgtccaatat actttgaaaa cttactccct cgcttgccag acggcgatgc
      661 aatgtggaaa cagaaatgta gagata
//
LOCUS       DAJOSY010000344          662 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJOSY010000344 DAJOSY010000000
VERSION     DAJOSY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.350"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(143..298)
                     /locus_tag="OHN27_004627"
     CDS             complement(143..298)
                     /locus_tag="OHN27_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288916.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
     gene            complement(393..>662)
                     /locus_tag="OHN27_004628"
     CDS             complement(393..>662)
                     /locus_tag="OHN27_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288917.1"
                     /translation="NGLRLFSLSIPEPSVKNLFAVNEFYRNNDDFINPDLQERLVIGD
                     DSISIFTYDIKSNFFEIRDNIGTENIFSSFSDFSSFLNEIMDSCS"
BASE COUNT          219 a          103 c          105 g          235 t
ORIGIN      
        1 gcaaagacgc gttcatcttc gtcaaagata ttattaaatg ctggttttga aaaaagttca
       61 tcggataaga tatagtcacg aaataagtat ctgctaatat tttatgattt ctttttcaga
      121 acgtcgaacg ggattttctt atttaagata tcagaatctt atgattcggg taaaattcta
      181 aataaatcaa catgtcatta aaatcatcat agccattagc tatttcggct aaaatagaga
      241 ctacatgtct tcggtccatc tcacttaagg agtgtagttc cgttgtaagt ttttccatag
      301 cttgcactgc taaatttcga acaaggaatt ttctgctggt aatctctaaa aagatggcat
      361 ggtttacaat gatttttgtt tccttttgat tattatgaac aactgtccat gatttcgttt
      421 aagaatgaag agaaatcact aaacgaactg aatatatttt ctgtgccaat attatctcta
      481 atttcaaaaa agttactttt aatgtcataa gtaaatattg aaatgctatc atccccgatc
      541 actaaccgtt cttgtagatc agggtttatg aaatcatcat tatttctata aaattcattt
      601 acggcaaaaa ggtttttaac tgaaggttct ggaatcgaga ggctaaataa tcgtaatcca
      661 tt
//
LOCUS       DAJOSY010000345          653 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJOSY010000345 DAJOSY010000000
VERSION     DAJOSY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.351"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..570)
                     /locus_tag="OHN27_004629"
     CDS             complement(<1..570)
                     /locus_tag="OHN27_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288918.1"
                     /translation="MLFMSDAPVKFRIDYILSQEYFYVHYLLAPVFFGSVLAVITPYA
                     QWLLSLAQKWATDKHSENIYLSKEKEYLNSIRLTGLKVRVAREEEKENAKIDADIKAE
                     VERGKREELVTEDLETARKQMLKEISNLKESVSIEKQVIENIAKEKERLQDLIVASLD
                     VMNDFFKVDNSRSLQQLKSRVEELLTVSDI"
BASE COUNT          200 a          139 c           88 g          226 t
ORIGIN      
        1 aatatcagaa actgttaaaa gttcttctac cctactcttc aattgctgta atgaacggct
       61 attatcaact ttaaagaaat cattcattac atcaagtgag gcaacaatca aatcctgtag
      121 tctctctttt tcttttgcta tattttcaat aacttgtttt tcaatagata ctgactcttt
      181 taagttgcta atctctttaa gcatctgttt ccttgctgtc tcaaggtctt ctgtgaccaa
      241 ctcttcacgc ttaccacgct ctacttccgc tttaatatca gcatcaattt tagcattttc
      301 tttctcttct tctctggcaa cccgaacctt taaccccgtt aacctgattg aattaaggta
      361 ttccttttct tttgaaagat aaatattttc actgtgttta tctgtagccc atttttgagc
      421 aagagaaagt aaccattgag cataaggagt aataacagct agcacacttc caaaaaagac
      481 aggtgctaac agataatgta cataaaaata ttcctgagac agaatataat ctattcgaaa
      541 cttaacagga gcatcactca taaagagcat tgctaagtta ggccaattga accataccca
      601 tgcaagaatg atatatcccc ataatgggct tttaatccta tcgtttattg ctt
//
LOCUS       DAJOSY010000346          650 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJOSY010000346 DAJOSY010000000
VERSION     DAJOSY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.353"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            146..562
                     /locus_tag="OHN27_004630"
     CDS             146..562
                     /locus_tag="OHN27_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288919.1"
                     /translation="MFRFLRRPLAEVANYSNDRWEAPQRASRLAASVKRYKTSEMLRF
                     IFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHRSADSNPEMVEK
                     MAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKK"
BASE COUNT          161 a          160 c          173 g          156 t
ORIGIN      
        1 tcgatgactc gctattgcag tacctgtcgc cactcggctg ggagcacatc aacctgaccg
       61 gtgattacct atggcgcagc agcgccaaga tcggcgcggg gaagttcagg ccgctacggc
      121 ctctgcaacc ggcttagcgt gctttatttt ccgttttctg agacgacccc tggctgaagt
      181 ggctaattac agtaacgacc gctgggaagc accgcaacgt gcctcacgcc tggctgccag
      241 cgtaaagcgt tacaagacat cagaaatgct gcgctttatt tttgctacca ttgcctacga
      301 tcccgatcca gatttaaccc cgctgactgt gagacgtttg tgcaaggcat tgttcggcag
      361 aaccggaagc cagtggctgg ttgttgaagt ttttggggag aaggggcgtc agcaccgcag
      421 cgcggacagc aatccggaga tggtggaaaa aatggctgcc agatatcgtc atgccgccga
      481 actacactgg tccgcaacgc tggcagaaat tgaacgggta aagcggcttt atcagacgaa
      541 aattaagaaa tccaaaaaat aagtgggttg actgccattg ttgttcttct ccttcatcat
      601 taatgctaat gtatgcatac attagcatta atgatggtgc gttaaagtat
//
LOCUS       DAJOSY010000347          632 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJOSY010000347 DAJOSY010000000
VERSION     DAJOSY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.354"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>632
                     /locus_tag="OHN27_004631"
     CDS             <1..>632
                     /locus_tag="OHN27_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCQ2288920.1"
                     /translation="DYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRI
                     PYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVI
                     RTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRYLYDPLGRRVAKRVWRRERDLTGW
                     MSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGE"
BASE COUNT          151 a          208 c          182 g           91 t
ORIGIN      
        1 cgattacacc tggaacgaca acggcgaact catccgcatc agcagcccgc gccagacccg
       61 gagttacagc tacagcacca ccggcaggct gaccggcgtt cacaccaccg cagcgaatct
      121 ggatatccgc atcccgtatg ccacggaccc ggcgggcaac cgcctgcccg acccggagct
      181 gcacccggac agcaccctca gcatgtggcc ggataaccgt atcgcccgtg acgcgcacta
      241 tctttaccgg tatgaccgtc acggcaggct gacagagaaa accgacctca tcccggaagg
      301 ggttatccgc acggatgatg agcggactca ccggtaccat tacgacagtc agcaccggct
      361 ggtgcactac acgcggacac aatatgaaga gccgctggtc gaaagtcgct atctttacga
      421 cccgctgggc cgcagggtgg caaaacgggt atggcggcgt gaacgggacc tgacgggctg
      481 gatgtcgctg tcacggaaac cgcaagtgac ctggtacggc tgggacggcg accggctgac
      541 cacaatacag aacgacagaa cccgcatcca gacgatttat cagccgggga gcttcacgcc
      601 actcatcaga gttgaaaccg ccaccggtga gc
//
LOCUS       DAJOSY010000348          619 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJOSY010000348 DAJOSY010000000
VERSION     DAJOSY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.355"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..560
                     /gene="espX1"
                     /locus_tag="OHN27_004632"
     CDS             <1..560
                     /gene="espX1"
                     /locus_tag="OHN27_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HCQ2288921.1"
                     /translation="ELNEQNIQYLTALNINIHKMLLSNITIEKSNLSYGYYFCCVLTN
                     ISCFESDLSNTIFSNGEINNLFIKKSNIFGTSFTNTMIKNLRCEDIMPGRWTTQLVNK
                     HLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELKKFMHDYDKT
                     SPEMRKYLQSINESMFLMKNIVHQD"
BASE COUNT          240 a          107 c           93 g          179 t
ORIGIN      
        1 atgaacttaa cgagcaaaac atccagtatt tgactgccct taatattaat atacataaaa
       61 tgttgctatc aaatataaca atagaaaaga gcaacctttc atatgggtat tatttttgtt
      121 gtgtattaac caatatatca tgttttgaaa gcgacttatc aaacaccatc ttttcaaatg
      181 gagaaataaa caacctcttc attaaaaaaa gcaacatatt tggaacatcg ttcactaata
      241 ccatgataaa aaatttgcgc tgtgaagata ttatgcctgg ccgatggacc actcaacttg
      301 tcaataagca cttaggttac agatacacag gagtattcaa aaccctggca agtattgatg
      361 acaaaccgtc ccgtttcgaa attttgatac cattggtaca aactttagtt cgcgacaatg
      421 taaagctaaa taatgatgtg tataaagaac tgaaaaagtt tatgcacgat tacgacaaaa
      481 catccccaga aatgagaaaa taccttcaga gcatcaacga gtctatgttc cttatgaaaa
      541 acatagttca tcaagattaa aatactcaaa gaatgaggta atttcagaat gaaattaact
      601 gtgatgatta ttaattcac
//
LOCUS       DAJOSY010000349          609 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJOSY010000349 DAJOSY010000000
VERSION     DAJOSY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.356"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..425)
                     /locus_tag="OHN27_004633"
     CDS             complement(<1..425)
                     /locus_tag="OHN27_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2288922.1"
                     /translation="MFPSIGLPLLLVASISNNAHAASGQGVVNFTGTVVDAACSLAAD
                     SADQTVDFGQISKAVLDADGISEKKNFDIKLTGCELNNTAKIVTLSFVGTTYNGVTQE
                     LGTSGNTNTAIVLSDQGGNLVSFDNTAGSATTLQDGDNT"
BASE COUNT          225 a          132 c           93 g          159 t
ORIGIN      
        1 aaggtattat ctccatcctg gagagtggta gctgaaccag cggtgttatc aaaagagacc
       61 aagtttccac cttgatcaga taatacaata gctgtattag tattacccga tgtgcctaac
      121 tcctgagtga caccattata ggtagttccg acaaatgaaa gtgtaactat ttttgcagta
      181 ttattaagct cacatccagt taatttaata tcaaaatttt tcttctccga gatcccatct
      241 gcatctaaca cggctttaga tatctggcca aaatcaacag tctgatcggc cgaatcagct
      301 gcaaggctac atgcagcatc aacgacagtt ccagtaaaat ttacaacccc ttgccctgag
      361 gccgcatgtg cattattcga aatactcgca acaagtaata acggtaaacc aatagaggga
      421 aatatattct ttttgatggc tattttagac tccacaataa aaactctatc taactatgag
      481 gggaaatatg ccctctcaca tacatcccac tcacctttta ataataccat tcaaaaaaca
      541 aaaaaatatc aaaaaaaagc aagaaaacac aaaaaaagcg aacgcaagaa aacaaaaaat
      601 aatactatt
//
LOCUS       DAJOSY010000350          600 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJOSY010000350 DAJOSY010000000
VERSION     DAJOSY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.357"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            21..512
                     /locus_tag="OHN27_004634"
     CDS             21..512
                     /locus_tag="OHN27_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCQ2288923.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          223 a           95 c          101 g          181 t
ORIGIN      
        1 acacatcaca gaggaatatt atgagcacag aaacaattga aatattcaat aatagtgatg
       61 aatgggcaaa tcaactaaaa cacgcattat cgaaaggaga aaatctggca ttactacatg
      121 gtttaactcc tgatatcctt gatagaatat atgcatatgc attcgactac catgaaaagg
      181 gtaatataac ggacgcagaa atttattata aatttttgtg catttatgcg ttcgaaaatc
      241 atgagtatct aaaagatttt gcatcagtat gtcagccaaa aaagaaatat caacaagcat
      301 atgaccttta caaactaagt tacaattact ccccgtatga tgactattca gttatttatc
      361 gtatgggtca atgtcagatt ggggctaaaa atatcgataa cgcaatgcaa tgtttctatc
      421 acattattaa caattgtgag gatgatagtg ttaagagtaa agcgcaggca tatattgaac
      481 tcttaaacga taattcagaa gataatggct aagcattaca atcatttggt taaatttgct
      541 ggttatagtg cagttaacca ctataaccag cacctattat gttttcttaa atcagcatct
//
LOCUS       DAJOSY010000351          594 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJOSY010000351 DAJOSY010000000
VERSION     DAJOSY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.358"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          197 a          128 c           81 g          188 t
ORIGIN      
        1 tctcagacat tcatgcagac tgaatgttga ttacccgttt tcacatcagg catggttctt
       61 tactgaccaa aaaacaaact ggtttactct tgaattattt tctctacctc agaaaacatg
      121 gtctcaggaa tctgctatgg tttcatagtt tctgttgttc ttttcataaa agttataagt
      181 cattgtcata atcatacgat aatcacttta tgttagtttt tactgtcaca cgctactgac
      241 tttttcagtc attgccagaa aggggctctt tttacttcca tttcaccact caacaaatgc
      301 aaaacttgtc cagtaaagcg gaccacctct tgctcacaag caacgaatca tctccaggaa
      361 cagccctcaa acattgcaga aacaaaaaca acaaaaccct cattatcact gatgttcgga
      421 aaatttcccg aaacaaaaaa aacgaaatca aaatgagact cattatcatg gatgtagtca
      481 aaaagacgtt atggtggctt tcagttaaat ggtactattt ctcataaatc cacttaactt
      541 tttttactga ttcatcccag ataaccactt tatcagtaaa aaatgaatta tata
//
LOCUS       DAJOSY010000352          590 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJOSY010000352 DAJOSY010000000
VERSION     DAJOSY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.359"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            47..379
                     /locus_tag="OHN27_004635"
     CDS             47..379
                     /locus_tag="OHN27_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCQ2288924.1"
                     /translation="MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            390..>590
                     /locus_tag="OHN27_004636"
     CDS             390..>590
                     /locus_tag="OHN27_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288925.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVA"
BASE COUNT          168 a          127 c          156 g          139 t
ORIGIN      
        1 cctcctggtc agggcttcgc cccgacaccc cagtaaggag ctgaccgtga gtgataaacg
       61 tcaaagaagt ggtagtgaaa gacggcaaaa gacgatcctg agagcggtcc ggttctcacc
      121 ggatgaggat gaaactatct gtaagaatgc tgaagattcc ggacttactg tatctgctta
      181 catccgaaat gcagcactga ataagcgaat taactcccgt acagatgatg cttttctgaa
      241 ggagttaatg agactgggaa ggatgcagaa gcatctgttt gttcagggaa aaagaaccgg
      301 tgacaaggaa tacgcagaag tgcttgttgc cataaccgaa cttacaaata cattgcgtaa
      361 acagttaatg gaaggttgag gattctccgg tgaatgcagt cattccgaaa aagagaaggg
      421 acggcaagtc ttcgttcgaa gacctggtat cctacgtctc tgtccgggat gacatgaccg
      481 atgaggaact ggatttatct tcttcttcgc aggctgaaca accgcaccga agccgtttca
      541 gtcgccttgt tgattatgcg acacgtcttc gaaatgagtc attcgtggcg
//
LOCUS       DAJOSY010000353          586 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJOSY010000353 DAJOSY010000000
VERSION     DAJOSY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.360"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            299..502
                     /locus_tag="OHN27_004637"
     CDS             299..502
                     /locus_tag="OHN27_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005188750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2288926.1"
                     /translation="MKEKNNFIRLPEVLERTGFCKAWIYRLISRDEFPSPIKIGDRAI
                     GFVESEIDAWIDEKIYLSRNKAA"
BASE COUNT          206 a           82 c          112 g          186 t
ORIGIN      
        1 ctggcgtccc tgtaatccac tctttaattg tttgattatt atatatatta atgatttgta
       61 ttttacattg aggtgcaata aagggataaa aaaagtattg tggctaaaaa tcaatcacat
      121 tttgaatgtc atgactagcg gtgaggagtt gaactcatga gttcaacttc cgaaagtgat
      181 tgaatgactg ttaatttaaa atagtcaaca aaaaaagcac tttttgctaa aaaagttgtt
      241 gatcgtcata agcgatattc gaataatagc ctctgtcgaa aaacaatggg agagataaat
      301 gaaagaaaaa aacaacttta tacgtctacc tgaagttctg gaaagaacag gattttgtaa
      361 agcttggatt tatcgtttga ttagtcgtga cgaattccct tcacctataa aaatcggtga
      421 tcgtgcaatc gggttcgtag aaagtgaaat agatgcgtgg attgacgaaa agatttattt
      481 atcaagaaat aaagccgcct gaatgttatt aaatgctttt ttgatttatt cctgacagat
      541 tcatcattaa aagtgaaaac ctctcaaaat cgcgtataga gcgttt
//
LOCUS       DAJOSY010000354          583 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJOSY010000354 DAJOSY010000000
VERSION     DAJOSY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.361"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            164..>583
                     /locus_tag="OHN27_004638"
     CDS             164..>583
                     /locus_tag="OHN27_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4755 domain-containing protein"
                     /protein_id="HCQ2288927.1"
                     /translation="MKTENIIFLFWAVIFILILCQLFYFGPKKRRYLNTYTEVLDGDV
                     LSYECQNTGVVIDTKKHTVRIFNTDKDSTFKYDNIREINYTLSEAGKIYSTGNNLNSM
                     IKSAGANSNEQMLANQRSGIFILTDDIKNPSWKINLPM"
BASE COUNT          205 a          105 c           97 g          176 t
ORIGIN      
        1 tcgctaaaaa ctatcaatat taaataattg atctgttata acgatcattt tacttgagag
       61 cagccctcct catggtatgc tcggagcgtg gaataccggg cgaatcacct tggtatcttc
      121 atgattaagc taaaaactat cgctctatga ggaatgaaat tttatgaaaa ccgaaaacat
      181 tatttttctt ttttgggcag tgatttttat cctgattctc tgccaactgt tttactttgg
      241 ccctaaaaaa agaagatatc tcaatactta tacagaggta ttagatggtg atgtcctctc
      301 atatgaatgt caaaacaccg gtgtagtcat tgatactaaa aaacacacag tacgtatttt
      361 caatacagat aaggacagca ctttcaaata cgacaacatc agggaaataa attatacgct
      421 atccgaagct ggaaaaattt atagcacagg taataatcta aactctatga taaagtccgc
      481 tggagctaac agtaatgaac aaatgttagc taatcagcgt tccggtattt ttatactgac
      541 agatgatata aaaaatcctt catggaaaat caatctacca atg
//
LOCUS       DAJOSY010000355          582 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJOSY010000355 DAJOSY010000000
VERSION     DAJOSY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.362"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>582)
                     /locus_tag="OHN27_004639"
     CDS             complement(<1..>582)
                     /locus_tag="OHN27_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCQ2288928.1"
                     /translation="HIKNNFKSNAAYTRNQGAKCAKTEWVCFLDSDDSFAADKLIKLA
                     HEIVENNHSNKTVFINQAVVYFNKEIEDIVPHRAIGEKEHISDYLFVSDEYMQTSTLT
                     IHSSFFERFGFNEEYVRHQDYDLCLTLYENGYTFKQLDFVGTNIYWGTHERPISKGES
                     VNYSLKWITENKGRVTECAYNNFMFKFVVMKCLR"
BASE COUNT          188 a          110 c           76 g          208 t
ORIGIN      
        1 cgtaggcatt tcatcaccac aaatttaaac ataaaattat tataggcaca ttctgttacc
       61 cttcctttgt tttcagttat ccatttcaac gagtaattta cagattcccc cttacttata
      121 ggtcgttcat gtgtacccca gtaaatatta gtccctacaa aatcaagttg tttaaacgtg
      181 taaccatttt cgtataaagt caggcataaa tcataatctt gatgccttac atactcttca
      241 ttaaacccaa atctttcaaa gaatgagcta tgaatagtta aagtagaagt ctgcatatat
      301 tcatcagaaa caaaaagata atcgcttata tgctcttttt ctcctattgc acggtgagga
      361 actatatctt caatttcctt attgaaatat accacagctt gattgataaa taccgtctta
      421 ttagaatgat tattctcgac tatttcatgt gcaagtttta ttaatttatc ggctgcaaat
      481 gaatcatcag aatctaaaaa acatacccat tcagtttttg cacatttagc accttggtta
      541 cgagtataag cagcatttga tttgaaatta ttttttatgt gc
//
LOCUS       DAJOSY010000356          563 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJOSY010000356 DAJOSY010000000
VERSION     DAJOSY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.363"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            80..532
                     /locus_tag="OHN27_004640"
     CDS             80..532
                     /locus_tag="OHN27_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapA/VapB family virulence-associated protein"
                     /protein_id="HCQ2288929.1"
                     /translation="MVTTMKQCSNLRSSIMDDVKAKYGEYLPKDQVSQILDKIASAEN
                     KYSAKTTLASAIFYIKVDTQITGEGGKHFSGNAGGLSSPGGGVLFGDLYTNDLEMLYK
                     NTVSFQITMTPVFCSVLFFDSSSNLLGHFEGGGASTISGVAGGTGSWS"
BASE COUNT          186 a           89 c          113 g          175 t
ORIGIN      
        1 attttggctt gaaaaaaatc tatttatgag caattaataa tattgcaatt gatgaattgc
       61 aatcccataa taacttgtaa tggtgaccac tatgaaacaa tgttcaaacc taagaagcag
      121 tattatggat gatgtaaaag caaagtatgg agagtatcta ccaaaagatc aagtttccca
      181 gattctagat aagattgctt ctgcagagaa taaatattca gctaaaacta ctttagccag
      241 tgcaattttt tatatcaaag tagatactca gatcactggt gaaggtggaa aacatttctc
      301 aggaaatgcg ggggggttat catctcctgg tggtggagtt ttatttggtg atctgtatac
      361 taatgatttg gagatgctat acaaaaatac tgtaagtttt caaataacaa tgacccctgt
      421 tttctgcagc gtattatttt ttgactcatc atctaacctc ctcgggcatt ttgagggagg
      481 gggcgcatca acaatatctg gtgttgcagg tggtacaggt tcatggtcct aatgtattaa
      541 aaaactaaca aatcagaccc gat
//
LOCUS       DAJOSY010000357          554 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJOSY010000357 DAJOSY010000000
VERSION     DAJOSY010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.364"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          186 a          106 c          109 g          153 t
ORIGIN      
        1 caattggtta ctatctatgg caagctcctg aattatgtgt gtgaaccagg agtttcccag
       61 gaatttcctc acaatgtggc actatcactg tcaccttttt ctccttggaa acctccaggg
      121 atgccccaaa gtttcggtca gtttttagat aggtagcatt cctcattaat aatgaggaca
      181 ccgagggtag aatcttaact ccgtgttaat agaataaaaa acaatcatca gaactgagta
      241 gggaaagcga atcatgatgt atgaagtttc acacacaatg tgaatcaaaa acgctcgtag
      301 ataatccgat agtgctgcct gatacgctga aagaaattgt tcgcaggaag atctgattat
      361 gctgattcgt cgaccacagg cccttatata cataagcagc gcccgcacct gaaatgcagg
      421 tcagaaacaa tgtgaacact atccgtgaga aattgtgaca aaattcccga tgatcgacaa
      481 gtgcaatgaa ataaaaaaca tatgttatat ataaatttga tatttatgaa aaatcaatat
      541 atttatcttg tgag
//
LOCUS       DAJOSY010000358          550 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJOSY010000358 DAJOSY010000000
VERSION     DAJOSY010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.365"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>550
                     /locus_tag="OHN27_004641"
     CDS             <1..>550
                     /locus_tag="OHN27_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCQ2288930.1"
                     /translation="DMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHT
                     LKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELK
                     HGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVH
                     DWLSLHRRLAEDGLYLSQMDGKF"
BASE COUNT          135 a          138 c          140 g          137 t
ORIGIN      
        1 gatatggagt acattgcccg gcaggcacac tatgcaaaag atgacactga ccctgttttt
       61 cactacatcc tttcctggca gtcacatgaa agcccgcgtc cggaacagat ttatgacagt
      121 gtacgtcata cgctgaaatc actcggcctt gccgaccatc agtatgtctc tgccgtgcat
      181 actgatacag ataatctgca cgtccatgtg gctgttaacc gggtacaccc tgaaacgggt
      241 tatctgaacc ggttatcatg gagtcaggaa aaactcagtc gtgcctgccg tgagcttgaa
      301 ctgaagcatg gttttgctcc ggataacggt tgctgggtcc atgcgccggg aaatcgtatc
      361 gttcgcaaaa ctgccgttga acgtgatcgc cagaacgcct ggacacgcgg aaaaaaacaa
      421 acttttcgtg agtatgttgc gcagacagcg gtcgctggtt tacgcagtga acctgtacat
      481 gactggttat ccctgcatcg tcgtcttgcg gaagatggtc tgtacctgtc tcagatggac
      541 ggaaaatttc
//
LOCUS       DAJOSY010000359          548 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJOSY010000359 DAJOSY010000000
VERSION     DAJOSY010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.366"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>548
                     /locus_tag="OHN27_004642"
     rRNA            <1..>548
                     /locus_tag="OHN27_004642"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          126 a          121 c          184 g          117 t
ORIGIN      
        1 acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa cggaggagca
       61 cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat aagccagctt
      121 gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc cggtggttct
      181 gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag gctgataccg
      241 cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat cacatcctgg
      301 ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg cgagctgggt
      361 ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga actgaggggg
      421 gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg ttgtcatgcc
      481 aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat ctaagcacga
      541 aacttgcc
//
LOCUS       DAJOSY010000360          535 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJOSY010000360 DAJOSY010000000
VERSION     DAJOSY010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.367"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>535)
                     /locus_tag="OHN27_004643"
     CDS             complement(<1..>535)
                     /locus_tag="OHN27_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCQ2288931.1"
                     /translation="RNSPVPVCVRNKNRKILYANGAFIELFSREDKPLSGESYIRLQV
                     EIFLSSLELECQALGHGSAFCRRFNFHGEIYQIRMENVSFYNDESVVLWQINPFPDYP
                     FFALNQSVSNTNTSGQLTIWNDLSPGTLVVFSFYMLGVGHATIARELGITDRASEDRI
                     KPVKRKIKEFFEHFDLF"
BASE COUNT          187 a          106 c           90 g          152 t
ORIGIN      
        1 ctgaataaat caaagtgttc aaaaaactct tttattttcc gtttaactgg tttaattcga
       61 tcctcagatg ctctgtctgt aatacccaat tctctggcaa ttgttgcgtg gccaacaccc
      121 agcatataaa aagagaaaac aaccaatgtt cctggagaaa gatcattcca tatcgttaat
      181 tgaccagaag tatttgtatt acttacactc tgatttaacg caaaaaatgg ataatcagga
      241 aacggattaa tttgccataa aacaacagat tcgtcattat aaaaagaaac attctccatc
      301 cttatctgat agatttcgcc atgaaaatta aaacgacgac aaaatgcaga gccatgtcca
      361 agagcctggc attccagttc aagtgatgaa agaaaaattt caacctgcag acgtatataa
      421 ctctctccgg ataaaggttt atcttctctg gaaaagagtt caataaaagc cccattggca
      481 taaaggattt tccggttttt gttacggaca caaaccggaa cgggagaatt tctgt
//
LOCUS       DAJOSY010000361          532 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJOSY010000361 DAJOSY010000000
VERSION     DAJOSY010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.368"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..375
                     /locus_tag="OHN27_004644"
     CDS             <1..375
                     /locus_tag="OHN27_004644"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335133.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose-4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCQ2288932.1"
                     /translation="TLLTDGNPHGHLILRGGREPNYGLSDITKAVKLMHDEGINHRLI
                     IDCSHGNSGKVAKRQISVARQVIDNRKKIPGYVAGIMVESFLQDGKQSDSFPLEYGQS
                     VTDECISWQQTEQLLSTLAAQL"
BASE COUNT          181 a           85 c          108 g          158 t
ORIGIN      
        1 acactattaa ccgatggcaa tccacatgga catctaatat tacgtggagg tagagaacct
       61 aactatggtt tgtctgatat aactaaggca gtgaaattaa tgcatgatga gggcattaac
      121 catcgtctga taattgattg tagccatggt aatagcggta aagtggctaa acggcaaatt
      181 tccgttgcac gtcaagttat agataatcgg aaaaaaatac caggatatgt agctggcatt
      241 atggtggaaa gctttttaca agatggaaaa caatccgata gttttccgct agagtatggt
      301 cagtctgtta ctgatgaatg catatcatgg caacagacag aacaattatt atcaactctg
      361 gcagcacagc tttaacttca actattgcac atatattgct tttaaagggg gaaaaatgaa
      421 attacttagt ttaagacact caattagtgt tatcagagaa gcagatattt tatctctaca
      481 gtttatgatt atataaagcg ctatcgtagc cgcgtattta atggtcttaa aa
//
LOCUS       DAJOSY010000362          529 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJOSY010000362 DAJOSY010000000
VERSION     DAJOSY010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.369"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..489
                     /locus_tag="OHN27_004645"
     CDS             <1..489
                     /locus_tag="OHN27_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCQ2288933.1"
                     /translation="FADEGHGIVKFKGEVISAPCSIKPGDEDLTVNLGEVADTVLKSD
                     QKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQSDLLKNTKETEIGGATGV
                     GVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSKDATPGNVYAQADYKIA
                     YE"
BASE COUNT          157 a          118 c          122 g          132 t
ORIGIN      
        1 tttgctgatg aaggtcacgg tattgttaaa tttaaaggtg aagttatctc tgctccatgt
       61 tctattaaac caggtgacga agatttaact gtaaatctgg gtgaagtagc agataccgta
      121 ctgaaaagcg atcagaaatc tttggcggaa cctttcacta ttcacctgca ggactgcatg
      181 ctgagtcagg gtggaactac ttattcaaaa gcgaaggtta ctttcactac agcaaatacc
      241 atgacgggtc agtctgatct tctgaaaaat accaaagaaa ctgaaatcgg cggcgccact
      301 ggcgttggcg tacgtattct ggacagccag agcggcgaag taactttagg tactccggta
      361 gtaattacgt tcaacaatac caacagctat caggaactga atttcaaagc tcgtatggaa
      421 tccccaagca aagatgccac cccgggcaac gtttacgctc aggctgacta caagattgct
      481 tacgagtaat cgcatttggc cgggttcacc cggccacatc attaacgga
//
LOCUS       DAJOSY010000363          522 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJOSY010000363 DAJOSY010000000
VERSION     DAJOSY010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.370"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            103..294
                     /locus_tag="OHN27_004646"
     CDS             103..294
                     /locus_tag="OHN27_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCQ2288934.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          141 a          118 c           91 g          172 t
ORIGIN      
        1 ccactgacgc ttttttacat aacgaagaat tgaccatttt gtcctgttgt gccttaatgt
       61 aagtaccgtc cacagcgtgg gacatacttc aaggaacctt ttgtgagtca ggcaaccagt
      121 atgcgaaaac gacaccgatt taacagtcgc atgacccgta tcgtactgct catcagcttt
      181 atcttcttct ttggccgttt tatctactcg tccgtcggtg cctggcagca ccatcagagc
      241 aaaaaagaag ctcagcaatc cacactctcc gtcgaatcac cggtacaacg ttagcggtta
      301 ccttctccac tttcacagaa cataacggca cttcgctgtc ggatgctttt gctgtttggg
      361 attatcaaag cggcagatat tctttcatct taaattttac gtctttatcc tgactgatgt
      421 ttatcctgtt tggctgcgaa ataaatataa aattaatata tatattgtaa tgatatattt
      481 ttataaatta ttccctgcgt gaattttaat aaatttaatc ta
//
LOCUS       DAJOSY010000364          503 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJOSY010000364 DAJOSY010000000
VERSION     DAJOSY010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.371"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            39..495
                     /locus_tag="OHN27_004647"
                     /pseudo
     CDS             39..495
                     /locus_tag="OHN27_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          129 a          134 c          133 g          107 t
ORIGIN      
        1 cccccataca gaatctgtct cttgatcaga tctcctgatt atgcatcgtg catatggtgc
       61 gcaacagcct gcgcttcgtg ttatggaagg actacaacgc cgtcactcgc gacctgaaag
      121 cggtttatca ggctcccacg gaagaggcag gccagcaggc actggaagcg ttcgctacgg
      181 cctgggacag tcgctatcct caaataagcc gaagctggca ggctaactgg ccgaatcttg
      241 ccacgttctt cgcttatcca acagacatcc gcaaagtgat ctatacgacg aatgcccccg
      301 agtcgctaaa cagcgtgatc cgtcatgcga ttaaaaaagc gtaaagtgtt cccgacagac
      361 gactaggtga aaaaagtagt gtggctggca atccagtctg cgtcccagaa atggacgatg
      421 ccgttgaagg actggcgaat ggcaatgagc cgctttatta tcgagttcgg tgaccgcctg
      481 gacggtcact tctgagaaaa ggc
//
LOCUS       DAJOSY010000365          501 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJOSY010000365 DAJOSY010000000
VERSION     DAJOSY010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.372"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>501)
                     /locus_tag="OHN27_004648"
     CDS             complement(<1..>501)
                     /locus_tag="OHN27_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCQ2288935.1"
                     /translation="KIECACNFLMDKDAQGYIDLSDLDLTSCHFKGDVISKVSFLSSN
                     LQHVTFECKEIGDCNFTTAIVDNVIFRCRRLHNVIFIKASGECVDFSKNILDTVDFSQ
                     SQLGHSNFRECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDYVRMST
                     GNFKDC"
BASE COUNT          175 a           99 c           73 g          154 t
ORIGIN      
        1 atgcaatctt taaaattccc tgtcgacatt cgcacataat caaacataac agctgtcaaa
       61 ttcgatttaa taaatgatat ttctttggca gacagaaact ctgctctggt gaagtgcgaa
      121 gcgtaaagat aacaattatc gaagtttgaa tttctaatct gacattcgcg aaaattacta
      181 tgaccaagtt gactctgcga gaagtcaact gtatcaagaa tatttttgct gaaatcgaca
      241 cattcaccac tcgctttgat aaaaatcaca ttgtgtaaac gtcgacatct aaatatgaca
      301 ttatcaacta ttgcagtagt aaaattgcaa tccccaattt ctttacattc gaatgttaca
      361 tgttgtagat ttgatgataa aaaagacacc ttcgatataa cgtcaccttt aaaatgacaa
      421 cttgttaaat ccaaatcaga caggtcgata tacccctgcg catctttatc catcagaaaa
      481 ttgcaagcgc attcaatttt t
//
LOCUS       DAJOSY010000366          499 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJOSY010000366 DAJOSY010000000
VERSION     DAJOSY010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.373"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..98)
                     /locus_tag="OHN27_004649"
     CDS             complement(<1..98)
                     /locus_tag="OHN27_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019303525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCQ2288936.1"
                     /translation="MNIKPLYDRVVIKRTEEEKVSAGGIVIPDSAT"
     gene            complement(338..>499)
                     /locus_tag="OHN27_004650"
     CDS             complement(338..>499)
                     /locus_tag="OHN27_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-VIa"
                     /protein_id="HCQ2288937.1"
                     /translation="DYLARTRVAQGPVGGAQSRLIDAADIVSFGIEWLEARHAAPAAA
                     ALKPKQRRD"
BASE COUNT           81 a          153 c          163 g          102 t
ORIGIN      
        1 tcggtggccg aatccgggat cacgatacca ccggcggaga ccttctcttc ctcggtgcgc
       61 ttgatgacga cgcggtcgta cagcggcttg atgttaatcg ccgcgcatat cttcctcgtg
      121 gggctaccca tcgcctttat cgcgcgccgc cgcgaccgtg cgcgcgcgca aaaattcgcc
      181 gcctgatcgc ctcgggcaat catccttgtc gagggtgcca agctgttctt tactgaagaa
      241 cgtcccgaca tccttgttga agaggcggcg ggcttgtggg cctgatgcta tgcggctggg
      301 cgagagggag gaatatatgt ccggaataat cgacgactca gtcgcggcgt tgtttcggct
      361 tcagcgctgc cgccgctggc gcggcgtggc gcgcctcgag ccattcgatg ccgaaggaaa
      421 cgatatcggc cgcgtcgatc agccgggatt gcgcgccgcc gaccgggcct tgcgcaaccc
      481 tggtgcgggc gagatagtc
//
LOCUS       DAJOSY010000367          494 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJOSY010000367 DAJOSY010000000
VERSION     DAJOSY010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.374"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            171..476
                     /locus_tag="OHN27_004651"
     CDS             171..476
                     /locus_tag="OHN27_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HCQ2288938.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT          125 a          128 c          129 g          112 t
ORIGIN      
        1 agagggggat agttaacaat cttatatttc cctctgcgcg cctttcctac ccgcagtata
       61 tagtgcaagc ggtatgagga aagggagatt tcacaaggat gttatactca aagtagacaa
      121 tcctgatctc aagcgtacgt attgtccatg cgatgaataa aggaaaagtt atgaaacaca
      181 agctttctgc aatccttatg gccttcatgt tgacgacgcc agctgcgttt gctgcccctg
      241 aagcaacaaa cgggaccgag gcaacgacgg gtactactgg caccacaacg accactaccg
      301 gcgcaaccac gactgctact accactggtg gcgtggctgc tggcgctgtc ggcacggcga
      361 cagttgtcgg tgtggcaacg gctgttggtg tggcgacgtt ggcagttgtt gcagcaaatg
      421 acagtggcga tggcggttct cataacacct caaccaccac gtccactaca cgctaatact
      481 gctttaacgc gtcg
//
LOCUS       DAJOSY010000368          492 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJOSY010000368 DAJOSY010000000
VERSION     DAJOSY010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.375"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>492)
                     /locus_tag="OHN27_004652"
     CDS             complement(<1..>492)
                     /locus_tag="OHN27_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239509.1"
                     /note="WcaJ; glucose-1-phosphate transferase responsible
                     for the addition of the first glucose-1-phosphate from
                     UDP-glucose to the lipid carrier undecaprenyl phosphate in
                     the biosynthesis of colanic acid; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCQ2288939.1"
                     /translation="SYKYVFIFLAVLVIFQMIGGITDFYRSWRGVKLSAEMALVIKNW
                     TLSILLTMGIVSFTPEIIIPFKIMLWWYISSFLGILLCRTLIRYGARWVRLLGYNTRK
                     VAIAGSMPVGISLAKSFIEEPWLGFVVNGYYDDVNKNESAIPYIGNYDILLNDAKAGK
                     IDR"
BASE COUNT          185 a          104 c           59 g          144 t
ORIGIN      
        1 attcgatcta ttttaccagc cttagcatca ttcaataaaa tatcgtaatt gccaatatat
       61 ggaatagccg actcattctt attgacatca tcataatatc cattaacaac aaatcctaac
      121 catggctctt cgataaaact cttagctaaa cttattccta caggcatact tccagcaata
      181 gcgaccttgc gagtattata acctaataat cttacccagc gagccccata tcttattaaa
      241 gtgcgacata atagaattcc caaaaacgat gatatatacc accacagcat gattttaaag
      301 ggaattatta tttctggggt aaatgaaaca atgcccatag taagtaaaat actaagagtc
      361 caatttttaa taaccagtgc catttcagct gatagtttta caccacgcca tgaacgatag
      421 aaatctgtta taccaccaat catttgaaaa attactaaaa cagctaaaaa tataaacaca
      481 tatttatagc ta
//
LOCUS       DAJOSY010000369          490 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJOSY010000369 DAJOSY010000000
VERSION     DAJOSY010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.377"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          148 a          112 c          109 g          121 t
ORIGIN      
        1 atttattggc tgctcgttag aagaacagca ttggtatgac tctgatgctc cttaaaatcc
       61 cacttttcag agtgggattt gtttaatgcc cagaacaggg ggaatttcca ttcatgccca
      121 aaaaatattt tgatgccctc ccaccaattc tcgcgttcag tccttcctga aaaacctcag
      181 aattcacgta gcgcatcgcc gctactgcat ccattaaaaa aactgacagt tttttaatca
      241 tcttgtgtga ctcaacttac tgaaaaaatg ggatttatga aaatgccacc gtcggcatca
      301 agcagttggt cagcgcaacc ggctgcatcg actgcaacaa aaaaactgaa agagaaagcc
      361 cctcaaatga gggagaaaaa aaggaagcgc agtcaacgag aggcgtaaaa aaacccgact
      421 ggtgaggtcg ggttttaata cggttccggt tctgagttgg tggctctgac cggaggaagt
      481 cactctgcac
//
LOCUS       DAJOSY010000370          488 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJOSY010000370 DAJOSY010000000
VERSION     DAJOSY010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.378"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..488
                     /gene="espX1"
                     /locus_tag="OHN27_004653"
                     /pseudo
     CDS             1..488
                     /gene="espX1"
                     /locus_tag="OHN27_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
BASE COUNT          201 a           88 c           67 g          132 t
ORIGIN      
        1 ttggatgttt ttgatgacag aacaagcgaa tgtctttctg aagagattaa gatataccaa
       61 gaatgccatg aaaaattcgt taatttttta aaggcaaact tcaaccaaga aatataccca
      121 cagctatata ctcctgaaat attttatgag gcctgcagaa atctccagtc attttatgat
      181 catcaggaaa cgtcacaaaa cgcaaaatac agtgcaatag acaagaaaaa aagtcatctt
      241 aaccaggaaa tcaaaaagct tattaaaaaa aatattttcc ctgagctata caatgagcaa
      301 tgcaataaaa tcccctcctc atcgatagat gataaccagg gaataatgtg gcagaacttt
      361 aaaactagca atttgcatat tcacaactat gtaaaaaatt atctttattt aaaagcatac
      421 ccagtcgatt aataaaaaaa tcagcttact gctcaactga aaacacaatc accaataaat
      481 ttgatgtt
//
LOCUS       DAJOSY010000371          479 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJOSY010000371 DAJOSY010000000
VERSION     DAJOSY010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.379"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..181)
                     /locus_tag="OHN27_004654"
     CDS             complement(<1..181)
                     /locus_tag="OHN27_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCQ2288940.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAG"
BASE COUNT           54 a          170 c          155 g          100 t
ORIGIN      
        1 agcccgctac gggctttttc atgccctgcc ctagcgtcca agcctcacgg ccgcgctcgg
       61 cctctctggc ggccttctgg cgctcctgct gcggcgtccg ctcgtgggcc gtggcgcggg
      121 tccgcgcgcc ggcctcgtgc gcctggcgct cgcgggcgag gtccagggcg gccgtcttca
      181 cgttctgcct tgcgcagatg agatagatct agcgtggact caaggctctc gcgaatggct
      241 cgcgttggaa actttcattg acacttgagg ggcaccgcag ggaaattctc gtccttgcga
      301 gaaccggcta tgtcgtgctg cgcatcgagc ctgcgccctt ggcttgtctc gcccctctcc
      361 gcgtcgctac ggggcttcca gcgcctttcc gacgctcacc gggctggttg ccctcgccgc
      421 tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga agccgtgtg
//
LOCUS       DAJOSY010000372          478 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJOSY010000372 DAJOSY010000000
VERSION     DAJOSY010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.380"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(157..312)
                     /locus_tag="OHN27_004655"
     CDS             complement(157..312)
                     /locus_tag="OHN27_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288941.1"
                     /translation="MREYPNGETTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAMRLLN"
BASE COUNT          152 a           94 c           83 g          149 t
ORIGIN      
        1 ctgttatgac tttcttggta cacagtagtg tcaattatat ggagcaaacg tataaaagat
       61 aagatgaaac atcgcaataa tcaacatacg acggtctaaa tttcacacaa acagataaag
      121 atgattatca tttattatca atgcattaga atcaaatcaa ttcaagagtc tcattgctgc
      181 ttccagaatt tcttctgaag ttacatgtcg atccgctgct acataaatga ctttatgatc
      241 tccggtcaga gatggaaacc ctgcagccat tacagtgagg tgtgttgttt cgccatttgg
      301 gtattcacgc atgatggtgt taactccggt catcactggc actaccattg ctggttcaga
      361 gttaaaaaaa actattattt ttttcatatg ttaccgtaat atgtgagtat ccatcgacta
      421 gacactaagc aaaaaagctc ccgaaggagc cttcattttc actttcttaa atctaacg
//
LOCUS       DAJOSY010000373          477 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJOSY010000373 DAJOSY010000000
VERSION     DAJOSY010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.381"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..365)
                     /locus_tag="OHN27_004656"
     CDS             complement(<1..365)
                     /locus_tag="OHN27_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HCQ2288942.1"
                     /translation="MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLE
                     LGLRVYDAQMERKESAFNQTEFNKLLLECAVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ASMVDDIREKVSVEMDRFFP"
BASE COUNT          143 a          110 c           81 g          143 t
ORIGIN      
        1 ggaaaaaacc ggtccatctc aacagacact ttctctctga tatcgtcaac catactggca
       61 tattcaaact tcgggtttcc ggagacatga gggctgagag actcaatacc taaaatcttt
      121 gctaccgttg actgtgtttt tacagcacat tcaagaagaa gtttattaaa ctctgtctgg
      181 ttaaacgcag actctttacg ctccatctgg gcgtcatata cacgaagccc taattcaaga
      241 agcattgaag cagttcctga aaggcttata tctttatctc ttgctccctc ctgacgacgc
      301 ttttcaacaa tcatgttaat tttttcatga acctcattac tgatatacag atttactctg
      361 gccataagac agtacctttt agattctgaa ataagtgttt tatttcatct actcatagta
      421 tggtctgaaa aaagaagagc acccctagca gcgcccctag gggcatacta taaaaaa
//
LOCUS       DAJOSY010000374          473 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJOSY010000374 DAJOSY010000000
VERSION     DAJOSY010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.382"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(30..407)
                     /locus_tag="OHN27_004657"
     CDS             complement(30..407)
                     /locus_tag="OHN27_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288943.1"
                     /translation="MKRLITAAFIILPLASCTVYGNKSIKDETQQNIASKIIKGKTTQ
                     QDILRFYGEPQTKETNDGKEMWGYSVMSGESQISNYIPGLALLKNSSTAHMKDLEIWF
                     KGDVVERYTFRQTASKTSRGLLD"
BASE COUNT          154 a           85 c           76 g          158 t
ORIGIN      
        1 tttatagata aaatggtatt cgatttagat taatctaaca aaccgcgaga agttttactt
       61 gccgtttgtc taaaggtata gcgttcaaca acatcacctt taaaccagat ctcaagatct
      121 ttcatgtgcg ctgtgctgct gttctttaat aatgctaatc ccggtatgta atttgaaatc
      181 tgggattcac cggacataac ggaatagccc cacatttctt taccatcgtt agtttcttta
      241 gtctgaggct ccccataaaa acgaaggata tcttgttgag tagttttacc cttgataatc
      301 ttagaggcta tattctgctg tgtttcgtct ttaatagatt tgtttccata aacggtacaa
      361 ctcgctaaag gaagaataat aaatgctgcc gtaatcaatc ttttcatatc aactccaaat
      421 ataaataata aaacataaca aggttataaa acgtatttat taaaaatgag aag
//
LOCUS       DAJOSY010000375          467 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJOSY010000375 DAJOSY010000000
VERSION     DAJOSY010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.383"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>467)
                     /locus_tag="OHN27_004658"
     CDS             complement(<1..>467)
                     /locus_tag="OHN27_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCQ2288944.1"
                     /translation="DSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLFFNLDK
                     EPYENSRKLQELIWDKLVAYVNDFNLSNQEKSRLILRLFDDVKLLFNEVPVSILVNDI
                     FLKDFFMRQPDFAKWYFYQLLKKYEGEQLYLNELGYVYGNEEKTNEIEKKHPG"
BASE COUNT          157 a           89 c           55 g          166 t
ORIGIN      
        1 taacccggat gttttttttc aatttcattt gttttttctt cattaccata aacatatcct
       61 aactcattaa gatacagctg ttcgccctca tacttcttaa gtaattgata gaagtaccac
      121 ttcgcaaaat caggttgcct catgaaaaaa tccttcaaaa aaatatcatt tactaaaatg
      181 ctgacaggca cttcattaaa tagcaactta acatcatcaa ataaccttag tattaagcgt
      241 gatttttctt gattgcttaa gttaaaatca ttgacatacg caaccaactt atcccaaatc
      301 aattcctgta atttacggct attttcatag ggttctttat cgagattaaa aaataaggtc
      361 tcagctattt taattttaga ttgatcgcag ctaaaatctt tagattgatc gcagctaaaa
      421 tcaatatgat ttatcacaga gcatgcatac tttatctttt cagaatc
//
LOCUS       DAJOSY010000376          457 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJOSY010000376 DAJOSY010000000
VERSION     DAJOSY010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.384"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..299
                     /locus_tag="OHN27_004659"
     CDS             <1..299
                     /locus_tag="OHN27_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCQ2288945.1"
                     /translation="QQYLSQSCGAFVCMAAQEVIEQRESNSDSAPYTLLKNYADRFKK
                     YSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          156 a           92 c           90 g          119 t
ORIGIN      
        1 ttcagcaata cttaagccaa agttgcggtg cgtttgtgtg catggcagcc caggaagtga
       61 ttgaacaaag ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg
      121 acagatttaa aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg
      181 taaacagaaa ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac
      241 aattagaaat aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac
      301 atcattgtta atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct
      361 aaaaagtcat cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt
      421 ccagcgtatc gcggcacgcg attgctggtc tgttgat
//
LOCUS       DAJOSY010000377          456 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJOSY010000377 DAJOSY010000000
VERSION     DAJOSY010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.385"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..219
                     /locus_tag="OHN27_004660"
     CDS             <1..219
                     /locus_tag="OHN27_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HCQ2288946.1"
                     /translation="IKNITHVIDSEVKSLAESIRQEISTFSGMNRIYLTGGGAELIYP
                     HIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            219..>456
                     /locus_tag="OHN27_004661"
     CDS             219..>456
                     /locus_tag="OHN27_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCQ2288947.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDSLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVK"
BASE COUNT          155 a           99 c           96 g          106 t
ORIGIN      
        1 ataaaaaata ttactcatgt aatagacagt gaggtgaaat ctctggcaga aagcatcagg
       61 caggaaattt caacattctc aggaatgaac cgaatctacc tcacaggcgg gggagctgag
      121 cttatttacc ctcacataaa gcaatatttc ccgaatctga aagtgaacaa ggtagatgaa
      181 cctcagtttg cattggtgaa agcaatggtg cacgcgtaat ggaaagcagt gatccgaaaa
      241 aaagaaagaa agttgtggca tacctgcacc cgacacttta cccgcaggac agcctgacac
      301 aacaaacaat agactctctc ccgatacaaa tgcgaggaga cttctacaga caatctctta
      361 tttgcggagc agctctttat tctgttgacc cccgactgtt aacgctaatc agtggttttt
      421 tcagtgagaa aatcactgca gaaaatctgg taaaac
//
LOCUS       DAJOSY010000378          447 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJOSY010000378 DAJOSY010000000
VERSION     DAJOSY010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.386"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          166 a           71 c           74 g          136 t
ORIGIN      
        1 gtaaaatgaa taatgatccg taccgtacaa ctgcggataa tgtgataagc gggacgactt
       61 cagacgttat tagggaagga acagaaaatg ctatggttat gtgagaattt tgtttaaata
      121 ttactttaca gaaacgggtg tctgttcagt caaaaaaata atcacaagct caacctcttg
      181 tttcttggcg ttattcagga caggtacgca tatcacaacg agtaacaaaa acgtaaagtt
      241 aaaccaatat tcataatata tttttattca cagcaataaa taatccaatt aacattatta
      301 aaatgaatga ttgcataaga aatatatgct accgctccac cctgataaca tgatctgcac
      361 attgcacatc ttaacgttgt taaatactgg ctcaatagtg gatattttaa attaggaatt
      421 tatcaaattc acttgataaa tagattg
//
LOCUS       DAJOSY010000379          442 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJOSY010000379 DAJOSY010000000
VERSION     DAJOSY010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.387"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>442
                     /locus_tag="OHN27_004662"
     CDS             <1..>442
                     /locus_tag="OHN27_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase NikB"
                     /protein_id="HCQ2288948.1"
                     /translation="SWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEK
                     IAETESLQQYACRHLGERLPEMAREGRLENCLAIHRTLAEAGLWMRVQHGHLVICDGY
                     DHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERF"
BASE COUNT          117 a          110 c          117 g           98 t
ORIGIN      
        1 ctcctggtgt gcagaaaaac tcatgaaaaa aatgggtgac tacacgccag tgccaaaaga
       61 cattttcagc caggtggaag caccaggacg ctataatccg gacttcattg cagctgatgt
      121 tcgcccggaa aaaatcgctg aaacagaaag tctgcagcag tatgcctgtc gtcatcttgg
      181 agaacgtctg ccggaaatgg cacgggaagg acggctggaa aactgcctgg ctattcaccg
      241 cacactggct gaagcgggat tatggatgcg tgttcagcat ggtcacctgg ttatctgtga
      301 cggttatgat cataaccaga ctcccgttcg tgctgacagc gtatggtcac tgctgacgct
      361 cgacaatgtg aatcaactcg atggtggctg gcagcctgta ccaacagata ttttccgcca
      421 ggttacgcca acagaacgct tc
//
LOCUS       DAJOSY010000380          439 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJOSY010000380 DAJOSY010000000
VERSION     DAJOSY010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.388"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            108..>439
                     /locus_tag="OHN27_004663"
     CDS             108..>439
                     /locus_tag="OHN27_004663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288949.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFESWRKKLPAISGPVNLYIPTS
                     FKDKYLEVESYFVDKSIWNVHITFHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNH
                     CFSPDKVN"
BASE COUNT          153 a           57 c           78 g          151 t
ORIGIN      
        1 attcaaggtt attatgatgg ctatacaaaa ggcattattg atgtgatgga taattttata
       61 cccttgataa gcttgttgag ctcagaacta gaaaaaaaag aataaacatg ataaacgatc
      121 tgaagagtat attgttaaaa tcctcagagg aagttgatgt ttttatcaaa atttttgaaa
      181 gttggagaaa aaaattaccg gcaatatcag gtcctgtaaa tctctatata cctaccagtt
      241 tcaaagataa gtatcttgaa gtagagtcat attttgtcga caaatcaata tggaatgtcc
      301 atattacatt tcatgatgat aagcgttttg tgttttttac cgatcaattt attgctgaat
      361 ttagtccgca ggaatttgtg gataattgtg agcaatattt aattaataat cattgtttct
      421 cccctgacaa ggttaacga
//
LOCUS       DAJOSY010000381          439 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJOSY010000381 DAJOSY010000000
VERSION     DAJOSY010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.389"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            32..>439
                     /locus_tag="OHN27_004664"
     CDS             32..>439
                     /locus_tag="OHN27_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCQ2288950.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADDSPI"
BASE COUNT          100 a          130 c          125 g           84 t
ORIGIN      
        1 ttttttccgt cttttttcag gagaagcact catgccgtct gtatcccgct accgcacctg
       61 gcttgccgtg cctgcagatg aaatagaaga tctcaaaaaa gcacatcccc cgatgaacgg
      121 gcacacgccc gtcatctggg acaaggagca caaactctgg ttcgcccgcc cgggggcgga
      181 cctgtccagg ctggaccgct ggctgccccg gccacaggac gtctccatga acggcagtga
      241 tccggtcacc gagtttgccc aggttctgga gaatgccgga ctggttctga aagaactgcc
      301 ggtcatggac gggaaaatcc accgtgtccc gactgccgat gacaaaaaag gacaaaaaag
      361 tggcgcatat cgcggattcc tggacggcag acctgccggc tggtacaggg attatcgcag
      421 cgcggatgat tcgccgatc
//
LOCUS       DAJOSY010000382          437 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJOSY010000382 DAJOSY010000000
VERSION     DAJOSY010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.390"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>437)
                     /locus_tag="OHN27_004665"
     CDS             complement(<1..>437)
                     /locus_tag="OHN27_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCQ2288951.1"
                     /translation="HIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGC
                     GWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKV
                     QNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDG"
BASE COUNT          104 a          128 c          121 g           84 t
ORIGIN      
        1 tcaccatctt tcagtttgaa gcaaaccgcc gggatgcctt cgtccgggcg acccgtacag
       61 atgaactcat acggccccag tttggcgatt tcatccgcca gataagcggc aacctggtaa
      121 gaggcgttct gtactttggt atagccttca cgaccgaggc gcaggaattc atagtactgt
      181 gcaattacct gacccgccgg gcgggagaag ttgatggcaa aagtaccaat ttgaccaccc
      241 aggtagtcaa cgttgaacac cagttcctgc ggcagcgctt cttcgtcacg ccagataacc
      301 cagccgcagc ccagcggagc cagaccgaat ttatggcctg aagcactgat cgatttcaca
      361 cgcggcaggc ggaagtccca gacgatatcc ggggcgacga acggtgccag gaagccaccg
      421 ctggcagcgt cgatgtg
//
LOCUS       DAJOSY010000383          433 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJOSY010000383 DAJOSY010000000
VERSION     DAJOSY010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.391"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..375)
                     /locus_tag="OHN27_004666"
     CDS             complement(<1..375)
                     /locus_tag="OHN27_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HCQ2288952.1"
                     /translation="METGKIALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLF
                     FAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASILILKSDLRHKTTLYTINFM
                     LSLCAVIVTETRAAILVFPFFAL"
BASE COUNT          152 a           69 c           90 g          122 t
ORIGIN      
        1 taacgcaaag aatggaaaca ctaatatggc tgcacgcgtc tcagttacta tgacagcaca
       61 taaactaagc atgaagttaa ttgtatataa tgttgtttta tgtcttaagt cggattttaa
      121 aatgagaatt gatgccagta aagaaggaaa aaggatgatg tagcctgctg ttgtagcgtg
      181 ggaagctgat aatgcaacac ggtcgacgtt taaatagaaa tgttgccaca tggcatgcgc
      241 aaaaaaaagt aactgcgttg ccagggatgc gaacagaatt agtttcccaa attttatttt
      301 tgttctcatc tcatttttag caaaaagaac taagaaaata aaagcgctac acaaggcaat
      361 ttttccagtt tccattgggc cacgatatgc attggtataa acagagcctg aaattttata
      421 atgtgaatac cag
//
LOCUS       DAJOSY010000384          431 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJOSY010000384 DAJOSY010000000
VERSION     DAJOSY010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.392"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>431
                     /locus_tag="OHN27_004667"
     CDS             <1..>431
                     /locus_tag="OHN27_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288953.1"
                     /translation="DCNVSSNNIGIANVVDSKLTLSTINANVMYGIKLSDGANDNIFS
                     ALKIEWNGENNLYVKNAVNNVISASILDRSGKAGIYLENSEIILNEIIIRRSGGSSNI
                     PKESTHIYIKGGNAIINNIITKSGRNDDGKGKLSPDFSIYA"
BASE COUNT          152 a           64 c           80 g          135 t
ORIGIN      
        1 agattgcaat gtttcttcaa ataacattgg gattgcaaac gttgttgatt caaagcttac
       61 cttatccacg ataaacgcaa atgtaatgta tggtattaag ttaagtgatg gtgcaaatga
      121 caatatcttt tcagctctca agattgagtg gaatggcgaa aacaatcttt atgtaaaaaa
      181 tgctgtcaat aatgttattt ccgcatccat acttgatcga tctggtaagg ctggtatcta
      241 tctcgaaaac tccgaaatta ttttaaatga aatcataata aggcgaagcg gtggaagttc
      301 aaatataccg aaagaatcaa cccatattta tatcaaaggt ggtaatgcaa ttattaacaa
      361 tattataacc aagtctgggc gtaatgatga cggtaaagga aaattatctc cagattttag
      421 tatttatgct g
//
LOCUS       DAJOSY010000385          429 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJOSY010000385 DAJOSY010000000
VERSION     DAJOSY010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.393"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>429
                     /locus_tag="OHN27_004668"
     CDS             <1..>429
                     /locus_tag="OHN27_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350955.1"
                     /note="induced within macrophages; necessary for the
                     resistance to antimicrobials; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antimicrobial resistance protein Mig-14"
                     /protein_id="HCQ2288954.1"
                     /translation="ARKKQNCIIKESFSPKFEKTRRNEIQRFIRNGGEIKCISQLSDK
                     EISSSYISLFHSRFGGTLPCYEYDNLLMFISHLRELMFGHVLFWDNKPCAIDIVLKSE
                     SSCNVYYDVPNGAVLNDENCMKLSPGSVLMWLNIDKARRY"
BASE COUNT          142 a           66 c           84 g          137 t
ORIGIN      
        1 tagccagaaa aaaacaaaat tgtattataa aggaatcttt ttcacctaaa tttgaaaaaa
       61 ctcgccgaaa tgaaatccag agatttatca ggaatggcgg agagattaag tgtataagcc
      121 agttatccga taaagaaata tccagctctt atatatcact ttttcattcc cggtttggag
      181 gtaccctacc ttgctacgaa tatgataatc tgttaatgtt tatttctcac ttacgagaac
      241 ttatgtttgg ccatgtgcta ttctgggata ataaaccgtg tgctattgat atagtactga
      301 aatctgaaag ctcatgtaat gtttattatg acgttccaaa tggtgcagtt ctgaatgatg
      361 agaattgtat gaaactaagt ccgggaagtg tccttatgtg gctgaatata gataaagcac
      421 gaaggtatt
//
LOCUS       DAJOSY010000386          427 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJOSY010000386 DAJOSY010000000
VERSION     DAJOSY010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.394"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          125 a           72 c          102 g          128 t
ORIGIN      
        1 caatgctttt atctttgacc taaatcaata aaatgtatcg tttgatatgc ttttaaacaa
       61 aatagctaaa aaatcttttt agaggggcgc ttcgcttggg gtcactatga atgatttttc
      121 tctcgctaag agagcgctta cagaggtctt tcaaatctca atagccagcc gaggaacaca
      181 ggaaaccgag ggaagaaaaa ctattgtgat taccttgaac aaggtctgta aaggtcgttt
      241 aagaggtcgg ttaagaaagg agcctccctc tctggggtta tcgctcttct cccgataagt
      301 ggggcggtta agagggcttg aagttatctg tgaaggtact ggacagcctt atatattggg
      361 tgattacaac tcgttgaaaa ataagcattt ttagaagcta ctgttaaggt tgcagagttg
      421 tctgtga
//
LOCUS       DAJOSY010000387          425 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJOSY010000387 DAJOSY010000000
VERSION     DAJOSY010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.395"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>425
                     /locus_tag="OHN27_004669"
     rRNA            <1..>425
                     /locus_tag="OHN27_004669"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          111 a          102 c          121 g           91 t
ORIGIN      
        1 ataccagctg gctgcaactg tttattaaaa acacagcact gtgcaaacac gaaagtggac
       61 gtatacggtg tgacgcctgc ccggtgccgg aaggttaatt gatggggtca gcgcaagcga
      121 agctcttgat cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga
      181 aattccttgt cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc
      241 cacccgagac tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg
      301 gaaagacccc gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg
      361 ataggtggga ggcttggaag tgtggacgcc agtctgcatg gagccgacct tgaaatacca
      421 ccctt
//
LOCUS       DAJOSY010000388          420 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJOSY010000388 DAJOSY010000000
VERSION     DAJOSY010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.396"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..376)
                     /locus_tag="OHN27_004670"
     CDS             complement(<1..376)
                     /locus_tag="OHN27_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-Ib"
                     /protein_id="HCQ2288955.1"
                     /translation="MASVSQGKQIKSVDDALNAFDKFRNNLNKKYSIQDRMAISKALE
                     AINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRPFFVKLESLAAGRA
                     ASAVTAWTFSVMLGTPVGILGFA"
BASE COUNT          121 a          102 c           73 g          124 t
ORIGIN      
        1 ttgcaaaacc cagaatacct acaggggttc ccagcatgac ggaaaacgtc catgctgtaa
       61 ctgctgaagc agctcttcct gctgccagtg attcaagttt tacaaaaaat ggacgccagt
      121 tgtccgtttt tacagccttt tgtaactcca cagcaacatc ataacgatca ataacttttc
      181 cggtaaaacc aaatgcctta ctgaacagct taaaattctc cgccatatgg acctgattaa
      241 tagcttccag ggctttagaa atggccatgc gatcttgtat gctatatttc ttgttcagat
      301 tattacggaa tttatcaaaa gcgttcagtg catcatccac actcttaatc tgtttccctt
      361 gagatacaga agccagctcc ttagcaagtt cggatgcttg tttaccgaac tcatcatata
//
LOCUS       DAJOSY010000389          419 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJOSY010000389 DAJOSY010000000
VERSION     DAJOSY010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.397"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>419
                     /gene="tuf"
                     /locus_tag="OHN27_004671"
     CDS             <1..>419
                     /gene="tuf"
                     /locus_tag="OHN27_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2288956.1"
                     /translation="APEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGA
                     AQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEV
                     RELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILEL"
BASE COUNT           99 a          114 c          119 g           87 t
ORIGIN      
        1 gcgccggaag aaaaagctcg tggtatcacc atcaacactt ctcacgttga atacgacacc
       61 ccgacccgtc actacgcaca cgtagactgc ccggggcacg ccgactatgt taaaaacatg
      121 atcaccggtg ctgctcagat ggacggcgcg atcctggtag ttgctgcgac tgacggcccg
      181 atgccgcaga ctcgtgagca catcctgctg ggtcgtcagg taggcgttcc gtacatcatc
      241 gtgttcctga acaaatgcga catggttgat gacgaagagc tgctggaact ggttgaaatg
      301 gaagttcgtg aacttctgtc tcagtacgac ttcccgggcg acgacactcc gatcgttcgt
      361 ggttctgctc tgaaagcgct ggaaggcgac gcagagtggg aagcgaaaat cctggaact
//
LOCUS       DAJOSY010000390          410 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJOSY010000390 DAJOSY010000000
VERSION     DAJOSY010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.398"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            101..410
                     /locus_tag="OHN27_004672"
                     /pseudo
     CDS             101..410
                     /locus_tag="OHN27_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT           83 a          120 c          122 g           85 t
ORIGIN      
        1 ctgaaccagt cactctcttc gctcttttcc ctcgatctct ccctggtcag ccagcagttt
       61 ctctcccttg aatttgcgca ggtgctggac aaaatggcct aacggcgttg tctggacaat
      121 ggaatacggc ccgttcgatt tgccggtggc gcgaacggac ggtgaaggcc accgctggca
      181 gtaccgctac gataaagaca cgctgcagct cacagaagtc attaacccgc agggcgagtc
      241 ataccgttat attctggaca actgtggccg ggtgacggaa gagcgtgact ggggcggcgt
      301 ggtctggcgt taccgctatg acgctgatgg cctgtgtacc gccagggtca acggcctgga
      361 ggaaaccatc ctctacagcc gggacgccgc aggccgcctg gcagaagtca
//
LOCUS       DAJOSY010000391          407 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJOSY010000391 DAJOSY010000000
VERSION     DAJOSY010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.399"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..330
                     /locus_tag="OHN27_004673"
     CDS             <1..330
                     /locus_tag="OHN27_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288957.1"
                     /translation="TYDTSFIDKDAEYIELLAKIDFSYEVDFGTSTTSIESKYLPSLT
                     DFIAQSLSQQLNCDIITSFKPLSGDEFCPITYFSKGKEVINFTTFNSNCWDGVCWVKK
                     RRQTNIR"
BASE COUNT          146 a           69 c           68 g          124 t
ORIGIN      
        1 acttatgaca catcttttat tgacaaagat gcagaatata ttgaattact agcgaagatt
       61 gatttttctt atgaagttga tttcggcaca tcaacaacat cgattgaaag caagtatctt
      121 ccatctctaa ctgatttcat cgctcaatca ctatcacaac aattaaattg tgacataata
      181 accagcttta aaccattaag tggagatgag ttttgcccaa taacatattt ttcaaaaggt
      241 aaagaagtta tcaattttac gacatttaat tcaaattgtt gggatggggt atgctgggta
      301 aaaaaaagga ggcaaacaaa tatccgctaa cgtagttttt caatagctac accatgtcac
      361 gtaacatgat gcctggatat taaccaggga aagatcatca gaagttc
//
LOCUS       DAJOSY010000392          403 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJOSY010000392 DAJOSY010000000
VERSION     DAJOSY010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.401"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          114 a           69 c           94 g          126 t
ORIGIN      
        1 gccgactttt aacttacgtt aaataccact tccagaagat tttaatcttt aataaagcaa
       61 taacgctaat cctgtgtatc gggtataaga atgaaggttc ctcaagtgtt tggcaattct
      121 tatgttattg tggcagtgcc ggatgacgcg tagctgccaa cctgaatcgc ataatctaaa
      181 aaatttacaa actgtagtat agactcttta gtaaaacgag agtcgtttga aagtttcatg
      241 atttgtgttt gcgcgggtcg tgggggaagt atcaggatgg aaacgttctg aatattttca
      301 aggcgcaatg agcatctcct atctctctat tttagccttt ttggcgtggg gggtaggggg
      361 ggaatgctta aagaaaaact atttcctcct tcggaggaaa act
//
LOCUS       DAJOSY010000393          387 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJOSY010000393 DAJOSY010000000
VERSION     DAJOSY010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.402"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          141 a           53 c           72 g          121 t
ORIGIN      
        1 aagaggaaca ttggaatact tatttgatga aattattgct ggcgagtgtg cattggcctt
       61 attacaaaaa gaaataaaaa aagattaatc caatatgtag aacgggacaa tacccatctt
      121 ttaacaatga acttcgtaat ttttatctta gaaataatgc tacattatat gaggtatatc
      181 atgaggtaaa aaatgttaac aagggagttg gcctggtggg agataatggt atcgtcctgg
      241 taattagaag ttccaattcc aaaaatacac ttgaggaatt tcatgaattc tttggaaact
      301 tcaaacctaa taattcaaca gctcgagttg aagtgattga tcaatccgtt ttagaacctt
      361 tttggagatt ttaaaaatat gatcacc
//
LOCUS       DAJOSY010000394          385 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJOSY010000394 DAJOSY010000000
VERSION     DAJOSY010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.403"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>385
                     /locus_tag="OHN27_004674"
     CDS             <1..>385
                     /locus_tag="OHN27_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HCQ2288958.1"
                     /translation="ILDEAVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDL
                     QYFKLQLLDASNLRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLES
                     QAIETLSAIGLSYEKYKTANNVYFN"
BASE COUNT          139 a           61 c           72 g          113 t
ORIGIN      
        1 atattttgga tgaagcagtc tgggtcaaac aagcactgga aaaaaatcaa ccaggaaagc
       61 cagtacgggt gataaatcct gatgatgaat cgatacgaat tttttcatgg cttgctgata
      121 atttccctga tttacaatat tttaaacttc aactattaga tgccagtaat ctaagattga
      181 cagtgagtaa gcaacggaac gctatcacac agcaactaat cgataatctt attaaaggat
      241 tactgcaaac tatgccatat gccagcaata taagtattgc ggtattagat gataatgtat
      301 tggaaagtca ggctattgaa acgttgtcag cgataggtct ttcttatgaa aaatataaaa
      361 cagctaacaa tgtgtatttc aatat
//
LOCUS       DAJOSY010000395          361 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJOSY010000395 DAJOSY010000000
VERSION     DAJOSY010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.404"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..190)
                     /locus_tag="OHN27_004675"
     CDS             complement(<1..190)
                     /locus_tag="OHN27_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCQ2288959.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSL"
BASE COUNT          127 a           44 c           69 g          121 t
ORIGIN      
        1 ataaactgaa aataaaaaag ctttgagctt ttaatttttg tgtacccata gaaaccataa
       61 aaaggagagc aatgtgagtg ctggatttta ttgatggcag agatatatag ttttgaccag
      121 gatcaatttt cgttaacgat aaaaaatatt taaagaataa ccccatggaa atacgtgttg
      181 actgaatcat ttttccgaat gattgttttc tggaactaaa ttatgtaaag gttatgctgt
      241 catatttaaa gcgattgtaa gctaatgtat gtaataaatg agataattta cagtaagcta
      301 aaaatcagcc aggcgataat gacctggctg atagttttag cctttagctg ttgcattttg
      361 t
//
LOCUS       DAJOSY010000396          361 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJOSY010000396 DAJOSY010000000
VERSION     DAJOSY010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.405"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..176)
                     /locus_tag="OHN27_004676"
     CDS             complement(<1..176)
                     /locus_tag="OHN27_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCQ2288960.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDR"
BASE COUNT           85 a           94 c           87 g           95 t
ORIGIN      
        1 cgatcatcag tcagtgagtt aactttctta ccgagagctg ccgcccgtaa aaaaccggaa
       61 acggtcttgc cgcattcagc agcccttttt ctgatttcct ggtcttcttc tgccgtcaga
      121 cgaacagggc gactgatagc ccgcctgcgc gtattacttc cacttcgttt tgtcataaaa
      181 catggctccg tatctgacat gggtgtcggg gcaaagccct gaccagggta attgtaatag
      241 cgtgcatgta tgcgcggtat aacaattgca catcctgtcc tgtcagcaag ctcgaattac
      301 cgacaaaggc acggcagttt tttggcagtg acagtgttgt ctgacagaaa tgcccgtcaa
      361 a
//
LOCUS       DAJOSY010000397          358 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJOSY010000397 DAJOSY010000000
VERSION     DAJOSY010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.406"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            21..>358
                     /locus_tag="OHN27_004677"
     CDS             21..>358
                     /locus_tag="OHN27_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2288961.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLLKWALCGHWGQSPRI
                     SDLAEQNKIIA"
BASE COUNT          100 a           83 c           88 g           87 t
ORIGIN      
        1 acctgaatca ggaattaaca atgaaacctg taaaaccacc tcgtattaat ggacgagtgc
       61 cggtcctgtc ggcacaggaa gcggtgaatt atattcccga cgaagcaaca ctttgtgtgt
      121 taggcgctgg cggcggtatt ctggaagcca ccacgttaat tactgctctt gctgataaat
      181 ataaacagac tcaaacgcca cgtaatttat cgattattag tccaacaggg cttggcgatc
      241 gcgccgaccg cggtattagt ccgctggcgc aagaaggtct gctgaaatgg gcattatgcg
      301 gtcactgggg acaatcgccg cgtatttctg atctcgcaga acaaaataaa attattgc
//
LOCUS       DAJOSY010000398          358 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJOSY010000398 DAJOSY010000000
VERSION     DAJOSY010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.407"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..242)
                     /locus_tag="OHN27_004678"
     CDS             complement(<1..242)
                     /locus_tag="OHN27_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCQ2288962.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          111 a           72 c           76 g           99 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgactttt
//
LOCUS       DAJOSY010000399          356 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJOSY010000399 DAJOSY010000000
VERSION     DAJOSY010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.denovo.408"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>356
                     /locus_tag="OHN27_004679"
     CDS             <1..>356
                     /locus_tag="OHN27_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCQ2288963.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAJOSY010000400         2979 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.guided.224, whole genome shotgun sequence.
ACCESSION   DAJOSY010000400 DAJOSY010000000
VERSION     DAJOSY010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.guided.224"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..245
                     /locus_tag="OHN27_004680"
     CDS             <1..245
                     /locus_tag="OHN27_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HCQ2288964.1"
                     /translation="AMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            238..972
                     /gene="frdB"
                     /locus_tag="OHN27_004681"
     CDS             238..972
                     /gene="frdB"
                     /locus_tag="OHN27_004681"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCQ2288965.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            983..1378
                     /gene="frdC"
                     /locus_tag="OHN27_004682"
     CDS             983..1378
                     /gene="frdC"
                     /locus_tag="OHN27_004682"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCQ2288966.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1389..1748
                     /gene="frdD"
                     /locus_tag="OHN27_004683"
     CDS             1389..1748
                     /gene="frdD"
                     /locus_tag="OHN27_004683"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCQ2288967.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1811..2944
                     /locus_tag="OHN27_004684"
     CDS             1811..2944
                     /locus_tag="OHN27_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCQ2288968.1"
                     /translation="MFKTTLCALLITASCSTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNALQ"
BASE COUNT          739 a          777 c          786 g          677 t
ORIGIN      
        1 ccgcaatggc acgtaaagag tcccgcggcg cacaccagcg tctggacgaa ggttgcaccg
       61 agcgtgacga cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga
      121 ctcgcctgga gtacagcgac gtgaagatta ctacgctgcc gccagctaaa cgcgtttacg
      181 gtggcgaagc ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg
      241 gctgagatga aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca
      301 ccgcatagcg cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg
      361 ggctacatca aagacaacct ggcaccggac ctgagctacc gctggtcctg ccgtatggcg
      421 atttgtggtt cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc
      481 ctgcgtgatt acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc
      541 gatctggtgg tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc
      601 atcggcaact cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggcg
      661 aagtatcacc agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag
      721 tttggcctga acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac
      781 gaagatagcc gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc
      841 gtatggagct gtactttcgt gggctattgc tccgaagtct gcccgaaaca cgtcgatccg
      901 gctgcggcca ttcagcaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg
      961 aaaccacgct aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac
     1021 gtccacctgg tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc
     1081 ggttccggct gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg
     1141 cccggaagcc tgggcgggat tcgtcgactt tttacaaaac ccggttattg tgatcattaa
     1201 cctgatcact ctggcggcag ctctgctgca caccaaaacc tggtttgaac tggcaccgaa
     1261 agcggccaat atcattgtaa aagacgaaaa aatgggtcca gagccaatta tcaaaagtct
     1321 ctgggcggta actgtggttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg
     1381 agcctgagat gatcaatcca aatccaaagc gttctgacga accggtattc tggggcctct
     1441 tcggggccgg tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg ctggtgggta
     1501 ttctgctgcc gctggggctg tttccgggtg atgcactgag ctacgagcgc gttctggcgt
     1561 tcgcgcagag cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt
     1621 gtggtttaca ccgtatgcac cacgcgatgc acgatctgaa aatccacgta cctgcgggca
     1681 aatgggtttt ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtgtcgtta
     1741 caatctaacg catcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa
     1801 accagaccct atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac
     1861 atttgctgcc ccccaaaaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga
     1921 gcaacaaaag atccccggta tggcggtggc ggtaatttat cagggtaaac cttattactt
     1981 tacctggggc tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga
     2041 gttaggttcg gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg
     2101 ggaaatcaag ttaagcgatc ccgcaacaaa atactggcct gaacttaccg ctaaacagtg
     2161 gaacgggatc acactattac atctcgcaac ctacaccgct ggcggcctgc cattgcaggt
     2221 gccggatgag gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc
     2281 atgggctcca ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact
     2341 ggctgtgaag ccgtctggtt tgagttttga gcaggcgatg caaacacgtg tcttccagcc
     2401 actcaaactc aaccatacgt ggattaatgt accgcccgca gaagaaaaga attacgcctg
     2461 gggatatcgc gaaggcaagg cagttcatgt ttcgccaggg gcgttagatg ctgaaactta
     2521 tggtgtgaag tcgaccattg aagatatggc ctgctgggta cgaagcaata tgaatccccg
     2581 tgatatcaac gacaaaacac ttcagcaagg gatacaactg gcacaatctc gctactggca
     2641 aaccggcgat atgtatcagg gcctgggctg ggaaatgctg gactggccgg taaatcctga
     2701 cagcatcatt aacggcagtg gcaataaaat tgcactggca gcacgccccg taaaagcgat
     2761 tacgccccca actcctgcag tacgcgcatc atgggtacat aaaacagggg caaccggcgg
     2821 atttggtagc tatgtcgcgt ttattccaga aaaagagctg ggtatagtga tgctggctaa
     2881 caaaaactat cccaatccag cgagagtcac cgccgcctgg cagattctca acgctctaca
     2941 gtaaaatttc atcgggtccg aattttcgga ccttttctg
//
LOCUS       DAJOSY010000401         2912 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.guided.226, whole genome shotgun sequence.
ACCESSION   DAJOSY010000401 DAJOSY010000000
VERSION     DAJOSY010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.guided.226"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..268
                     /gene="prfH"
                     /locus_tag="OHN27_004685"
     CDS             <1..268
                     /gene="prfH"
                     /locus_tag="OHN27_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCQ2288969.1"
                     /translation="SGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(325..1782)
                     /gene="pepD"
                     /locus_tag="OHN27_004686"
     CDS             complement(325..1782)
                     /gene="pepD"
                     /locus_tag="OHN27_004686"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCQ2288970.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2043..2501
                     /gene="gpt"
                     /locus_tag="OHN27_004687"
     CDS             2043..2501
                     /gene="gpt"
                     /locus_tag="OHN27_004687"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCQ2288971.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2593..>2912
                     /locus_tag="OHN27_004688"
     CDS             2593..>2912
                     /locus_tag="OHN27_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2288972.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMV"
BASE COUNT          700 a          751 c          751 g          710 t
ORIGIN      
        1 gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg ccacgcatct
       61 ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta acaaacggct
      121 ggcgcgattg ctgattgcct ggaagctgga gcagcagcaa caggaaaata gcgcggcgct
      181 gaaatcgcag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc gacggacatt
      241 tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag gcgattaaac
      301 gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca gttcagtcag
      361 cagtgtccag taatgaccaa cgctttcgat gtgaacttgc tcatccggag agtgtggacc
      421 ggtgattgtt ggcccgatag aaaccatgtc catttccgga tacggttttt tgaacagacc
      481 acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca ggcgctgata
      541 ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc caggatatgc
      601 gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca tgctcaccac
      661 gtagtcttta ccgctgtcga tcagagaacg gatcagacag tgaatttcta cgttattgtc
      721 agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg ccacgtcgga
      781 gttgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat cgcgagattt
      841 cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca gatttttctc
      901 tttttctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag atttcagggc
      961 gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga tggcgttacg
     1021 cagtgtgcca ccgttgaaat cgataaggcg caggtccagc tcttccgcat gacccgccag
     1081 gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac cgccggagtg
     1141 accgcctttt agacctttta aggttaactt gaaggtttca aagccagctg gaaccgcttc
     1201 acgatccaaa tgcaggttgg aggtgaagtc gatacccccc gcgcaaccca tgtagatttc
     1261 accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt tgctctgtaa
     1321 gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt ccagcgggcc
     1381 gtgaaccacg ttttcgtcag ccagaaccgc cagcgcagac gccataccaa tgccgttatc
     1441 tgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag gctggatagg
     1501 atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat cgaggtgggc
     1561 ctgcaagacg accggtttac gattttccat acctgcggtg gcaggtttac gaatcaggat
     1621 attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac caacaatgta
     1681 ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt tggcaaaaat
     1741 atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc tccttgtcga
     1801 tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc aacacaagac
     1861 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     1921 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
     1981 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     2041 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     2101 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     2161 tggtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     2221 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     2281 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga
     2341 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     2401 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg
     2461 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac
     2521 tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg
     2581 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     2641 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
     2701 tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     2761 atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     2821 gatgctgaac gtaccgacga tgatttatac gatactgtga ttggctaccg tggtggcaac
     2881 tggatttatg agtgggccac ccaggcgatg gt
//
LOCUS       DAJOSY010000402         1639 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.guided.267, whole genome shotgun sequence.
ACCESSION   DAJOSY010000402 DAJOSY010000000
VERSION     DAJOSY010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.guided.267"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..699
                     /locus_tag="OHN27_004689"
     CDS             <1..699
                     /locus_tag="OHN27_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2288973.1"
                     /translation="ALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTV
                     VAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGP
                     VAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQ
                     VDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPAL
                     RRGLWSGAGQRADR"
     gene            complement(731..1591)
                     /locus_tag="OHN27_004690"
     CDS             complement(731..1591)
                     /locus_tag="OHN27_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCQ2288974.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
BASE COUNT          345 a          504 c          498 g          292 t
ORIGIN      
        1 gccttgaacg gcctcaattt cctgacgggc tgtttccttt tgccggagtc gcacaaaggc
       61 gaacgccggc cgttacgccg ggaggctctc aacccgctcg cttcgttccg gtgggcccgg
      121 ggcatgaccg tcgtcgccgc cctgatggcg gtcttcttca tcatgcaact tgtcggacag
      181 gtgccggccg cgctttgggt cattttcggc gaggatcgct ttcactggga cgcgaccacg
      241 atcggcattt cgcttgccgc atttggcatt ctgcattcac tcgcccaggc aatgatcacc
      301 ggccctgtag ccgcccggct cggcgaaagg cgggcactca tgctcggaat gattgccgac
      361 ggcacaggct acatcctgct tgccttcgcg acacggggat ggatggcgtt cccgatcatg
      421 gtcctgcttg cttcgggtgg catcggaatg ccggcgctgc aagcaatgtt gtccaggcag
      481 gtggatgagg aacgtcaggg gcagctgcaa ggctcactgg cggcgctcac cagcctgacc
      541 tcgatcgtcg gacccctcct cttcacggcg atctatgcgg cttctataac aacgtggaac
      601 gggtgggcat ggattgcagg cgctgccctc tacttgctct gcctgccggc gctgcgtcgc
      661 gggctttgga gcggcgcagg gcaacgagcc gatcgctgat cgtggaaacg ataggcctat
      721 gccatgcggg tcaaggcgac ttccggcaag ctatacgcgc cctaggagtg cggttggaac
      781 gttggcccag ccagatactc ccgatcacga gcaggacgcc gatgatttga agcgcactca
      841 gcgtctgatc caagaacaac catcctagca acacggcggt ccccgggctg agaaagccca
      901 gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta
      961 aacccgctcc gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg
     1021 ggattggcgg atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt
     1081 gccaggcggt aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc
     1141 cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg
     1201 cgtttggtgt caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg
     1261 ccatcaatcg tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct
     1321 gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca
     1381 agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc
     1441 ccgttggaat ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca
     1501 tcgcgaccgt catcggtgag aagttcggca ggtattgggt ggtgacaatg taggtgctgc
     1561 cccaaatggc aggtgctatc gctgtgaaca ataaatcggg gcagacggcg cttccagcgt
     1621 gacgaccatg ccgagcacg
//
LOCUS       DAJOSY010000403         1263 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.guided.292, whole genome shotgun sequence.
ACCESSION   DAJOSY010000403 DAJOSY010000000
VERSION     DAJOSY010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.guided.292"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            152..1012
                     /locus_tag="OHN27_004691"
     CDS             152..1012
                     /locus_tag="OHN27_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HCQ2288975.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          334 a          292 c          291 g          346 t
ORIGIN      
        1 acaaaaggct gcctcatcgc taactttgca acagtgccga aatgtgcgcg gaacccctat
       61 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctggta
      121 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacattttc gtgtcgccct
      181 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      241 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      301 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      361 taaagttctg ctatgtggtg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
      421 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      481 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      541 cactgctgcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      601 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      661 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
      721 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      781 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      841 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      901 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
      961 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1021 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1081 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1141 ccactgagcg tcagaccccg aagagcgcct tatgtgacat tttctcctta cgctcctcta
     1201 tgccgttctg catcctgtcc ggatgcgtta tatcccggtg cgattttccg cttcaaagcg
     1261 tgt
//
LOCUS       DAJOSY010000404         1058 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.guided.310, whole genome shotgun sequence.
ACCESSION   DAJOSY010000404 DAJOSY010000000
VERSION     DAJOSY010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.guided.310"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            110..457
                     /locus_tag="OHN27_004692"
     CDS             110..457
                     /locus_tag="OHN27_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HCQ2288976.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            451..>1058
                     /gene="folP"
                     /locus_tag="OHN27_004693"
     CDS             451..>1058
                     /gene="folP"
                     /locus_tag="OHN27_004693"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCQ2288977.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGL"
BASE COUNT          206 a          295 c          294 g          263 t
ORIGIN      
        1 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
       61 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg
      121 gctttttctt gttatcgcaa tagttggcga agtaatcgca acatccgcat taaaatctag
      181 cgagggcttt actaagcttg ccccttccgc cgttgtcata atcggttatg gcatcgcatt
      241 ttattttctt tctctggttc tgaaatccat ccctgtcggt gttgcttatg cagtctggtc
      301 gggactcggc gtcgtcataa ttacagccat tgcctggttg cttcatgggc aaaagcttga
      361 tgcgtggggc tttgtaggta tggggctcat aattgctgcc tttttgctcg cccgatcccc
      421 atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg tgttcggcat tctgaatctc
      481 accgaggact ccttcttcga tgagagccgg cggctagacc ccgccggcgc tgtcaccgcg
      541 gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg tcggaccggc cgccagccat
      601 ccggacgcga ggcctgtatc gccggccgat gagatcagac gtattgcgcc gctcttagac
      661 gccctgtccg atcagatgca ccgtgtttca atcgacagct tccaaccgga aacccagcgc
      721 tatgcgctca agcgcggcgt gggctacctg aacgatatcc aaggatttcc tgaccctgcg
      781 ctctatcccg atattgctga ggcggactgc aggctggtgg ttatgcactc agcgcagcgg
      841 gatggcatcg ccacccgcac cggtcacctt cgacccgaag acgcgctcga cgagattgtg
      901 cggttcttcg aggcgcgggt ttccgccttg cgacggagcg gggtcgctgc cgaccggctc
      961 atcctcgatc cggggatggg atttttcttg agccccgcac cggaaacatc gctgcacgtg
     1021 ctgtcgaacc ttcaaaagct gaagtcggcg ttggggct
//
LOCUS       DAJOSY010000405          870 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP243
            SAMN25142528-rid15606013.guided.321, whole genome shotgun sequence.
ACCESSION   DAJOSY010000405 DAJOSY010000000
VERSION     DAJOSY010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142528
            Sequence Read Archive: SRR17681121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453688.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453688.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:48:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,694
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 69 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 22 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142528-rid15606013.guided.321"
                     /isolate="PP243"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..776
                     /gene="aadA1"
                     /locus_tag="OHN27_004694"
     CDS             <1..776
                     /gene="aadA1"
                     /locus_tag="OHN27_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCQ2288978.1"
                     /translation="AEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDL
                     LVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQ
                     FGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEAL
                     NETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVI
                     LEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          216 a          222 c          235 g          197 t
ORIGIN      
        1 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
       61 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
      121 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
      181 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
      241 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
      301 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
      361 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
      421 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
      481 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
      541 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
      601 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
      661 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcccgc gcagatcagt
      721 tggaagaatt tgttcactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
      781 taacaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca
      841 ctaagcacat aattgctcac agccaaacta
//
